LOCUS       DAZFRJ010000001        73529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000001 DAZFRJ010000000
VERSION     DAZFRJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.001"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..222)
                     /locus_tag="ACHKUN_000001"
     tRNA            complement(146..222)
                     /locus_tag="ACHKUN_000001"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(186..188),aa:Arg,seq:acg)
     gene            complement(286..362)
                     /locus_tag="ACHKUN_000002"
     tRNA            complement(286..362)
                     /locus_tag="ACHKUN_000002"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Arg,seq:acg)
     gene            complement(560..636)
                     /locus_tag="ACHKUN_000003"
     tRNA            complement(560..636)
                     /locus_tag="ACHKUN_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Arg,seq:acg)
     gene            complement(640..732)
                     /locus_tag="ACHKUN_000004"
     tRNA            complement(640..732)
                     /locus_tag="ACHKUN_000004"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(696..698),aa:Ser,seq:gct)
     gene            complement(1048..1233)
                     /gene="csrA"
                     /locus_tag="ACHKUN_000005"
     CDS             complement(1048..1233)
                     /gene="csrA"
                     /locus_tag="ACHKUN_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO6658336.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1468..4098)
                     /gene="alaS"
                     /locus_tag="ACHKUN_000006"
     CDS             complement(1468..4098)
                     /gene="alaS"
                     /locus_tag="ACHKUN_000006"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO6658337.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4226..4726)
                     /gene="recX"
                     /locus_tag="ACHKUN_000007"
     CDS             complement(4226..4726)
                     /gene="recX"
                     /locus_tag="ACHKUN_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO6658338.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4969..6030)
                     /gene="recA"
                     /locus_tag="ACHKUN_000008"
     CDS             complement(4969..6030)
                     /gene="recA"
                     /locus_tag="ACHKUN_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO6658339.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6110..6607)
                     /gene="pncC"
                     /locus_tag="ACHKUN_000009"
     CDS             complement(6110..6607)
                     /gene="pncC"
                     /locus_tag="ACHKUN_000009"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO6658340.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6752..7837)
                     /gene="mltB"
                     /locus_tag="ACHKUN_000010"
     CDS             complement(6752..7837)
                     /gene="mltB"
                     /locus_tag="ACHKUN_000010"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO6658341.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8093..8656
                     /gene="srlA"
                     /locus_tag="ACHKUN_000011"
                     /gene_synonym="gutA"
     CDS             8093..8656
                     /gene="srlA"
                     /locus_tag="ACHKUN_000011"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO6658342.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8653..9612
                     /gene="srlE"
                     /locus_tag="ACHKUN_000012"
                     /gene_synonym="gutE"
     CDS             8653..9612
                     /gene="srlE"
                     /locus_tag="ACHKUN_000012"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO6658343.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9623..9994
                     /gene="srlB"
                     /locus_tag="ACHKUN_000013"
                     /gene_synonym="gutB"
     CDS             9623..9994
                     /gene="srlB"
                     /locus_tag="ACHKUN_000013"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO6658344.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9998..10777
                     /gene="srlD"
                     /locus_tag="ACHKUN_000014"
     CDS             9998..10777
                     /gene="srlD"
                     /locus_tag="ACHKUN_000014"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO6658345.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10882..11241
                     /gene="gutM"
                     /locus_tag="ACHKUN_000015"
     CDS             10882..11241
                     /gene="gutM"
                     /locus_tag="ACHKUN_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO6658346.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11308..12081
                     /gene="srlR"
                     /locus_tag="ACHKUN_000016"
                     /gene_synonym="gutR"
     CDS             11308..12081
                     /gene="srlR"
                     /locus_tag="ACHKUN_000016"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO6658347.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12074..13039
                     /gene="gutQ"
                     /locus_tag="ACHKUN_000017"
     CDS             12074..13039
                     /gene="gutQ"
                     /locus_tag="ACHKUN_000017"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO6658348.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(13036..14550)
                     /gene="norR"
                     /locus_tag="ACHKUN_000018"
     CDS             complement(13036..14550)
                     /gene="norR"
                     /locus_tag="ACHKUN_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO6658349.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14737..16176
                     /gene="norV"
                     /locus_tag="ACHKUN_000019"
     CDS             14737..16176
                     /gene="norV"
                     /locus_tag="ACHKUN_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO6658350.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            16173..17306
                     /gene="norW"
                     /locus_tag="ACHKUN_000020"
     CDS             16173..17306
                     /gene="norW"
                     /locus_tag="ACHKUN_000020"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO6658351.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17532..19784)
                     /gene="hypF"
                     /locus_tag="ACHKUN_000021"
     CDS             complement(17532..19784)
                     /gene="hypF"
                     /locus_tag="ACHKUN_000021"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO6658352.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19937..20464)
                     /gene="hydN"
                     /locus_tag="ACHKUN_000022"
     CDS             complement(19937..20464)
                     /gene="hydN"
                     /locus_tag="ACHKUN_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO6658353.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20613..21623)
                     /gene="ascG"
                     /locus_tag="ACHKUN_000023"
     CDS             complement(20613..21623)
                     /gene="ascG"
                     /locus_tag="ACHKUN_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO6658354.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21883..23340
                     /gene="ascF"
                     /locus_tag="ACHKUN_000024"
     CDS             21883..23340
                     /gene="ascF"
                     /locus_tag="ACHKUN_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO6658355.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23349..24773
                     /gene="ascB"
                     /locus_tag="ACHKUN_000025"
     CDS             23349..24773
                     /gene="ascB"
                     /locus_tag="ACHKUN_000025"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO6658356.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSITWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24887..25306)
                     /locus_tag="ACHKUN_000026"
     CDS             complement(24887..25306)
                     /locus_tag="ACHKUN_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6658357.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25303..25614)
                     /locus_tag="ACHKUN_000027"
     CDS             complement(25303..25614)
                     /locus_tag="ACHKUN_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO6658358.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25788..26531)
                     /locus_tag="ACHKUN_000028"
     CDS             complement(25788..26531)
                     /locus_tag="ACHKUN_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658359.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26574..27044)
                     /gene="hycI"
                     /locus_tag="ACHKUN_000029"
     CDS             complement(26574..27044)
                     /gene="hycI"
                     /locus_tag="ACHKUN_000029"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO6658360.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(27037..27447)
                     /gene="hycH"
                     /locus_tag="ACHKUN_000030"
     CDS             complement(27037..27447)
                     /gene="hycH"
                     /locus_tag="ACHKUN_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO6658361.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27444..28211)
                     /gene="hycG"
                     /locus_tag="ACHKUN_000031"
     CDS             complement(27444..28211)
                     /gene="hycG"
                     /locus_tag="ACHKUN_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO6658362.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(28211..28753)
                     /gene="hycF"
                     /locus_tag="ACHKUN_000032"
     CDS             complement(28211..28753)
                     /gene="hycF"
                     /locus_tag="ACHKUN_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO6658363.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28763..30472)
                     /gene="hycE"
                     /locus_tag="ACHKUN_000033"
     CDS             complement(28763..30472)
                     /gene="hycE"
                     /locus_tag="ACHKUN_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO6658364.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30490..31413)
                     /gene="hycD"
                     /locus_tag="ACHKUN_000034"
     CDS             complement(30490..31413)
                     /gene="hycD"
                     /locus_tag="ACHKUN_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO6658365.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31416..33242)
                     /gene="hycC"
                     /locus_tag="ACHKUN_000035"
     CDS             complement(31416..33242)
                     /gene="hycC"
                     /locus_tag="ACHKUN_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO6658366.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(33239..33850)
                     /gene="hycB"
                     /locus_tag="ACHKUN_000036"
     CDS             complement(33239..33850)
                     /gene="hycB"
                     /locus_tag="ACHKUN_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO6658367.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33975..34436)
                     /gene="hycA"
                     /locus_tag="ACHKUN_000037"
     CDS             complement(33975..34436)
                     /gene="hycA"
                     /locus_tag="ACHKUN_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO6658368.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34648..34998
                     /gene="hypA"
                     /locus_tag="ACHKUN_000038"
     CDS             34648..34998
                     /gene="hypA"
                     /locus_tag="ACHKUN_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6658369.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            35002..35874
                     /gene="hypB"
                     /locus_tag="ACHKUN_000039"
     CDS             35002..35874
                     /gene="hypB"
                     /locus_tag="ACHKUN_000039"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO6658370.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35865..36137
                     /gene="hypC"
                     /locus_tag="ACHKUN_000040"
     CDS             35865..36137
                     /gene="hypC"
                     /locus_tag="ACHKUN_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO6658371.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            36137..37258
                     /gene="hypD"
                     /locus_tag="ACHKUN_000041"
     CDS             36137..37258
                     /gene="hypD"
                     /locus_tag="ACHKUN_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO6658372.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            37255..38265
                     /gene="hypE"
                     /locus_tag="ACHKUN_000042"
     CDS             37255..38265
                     /gene="hypE"
                     /locus_tag="ACHKUN_000042"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO6658373.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38339..40417
                     /gene="flhA"
                     /locus_tag="ACHKUN_000043"
     CDS             38339..40417
                     /gene="flhA"
                     /locus_tag="ACHKUN_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO6658374.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(40454..40807)
                     /gene="ygbA"
                     /locus_tag="ACHKUN_000044"
     CDS             complement(40454..40807)
                     /gene="ygbA"
                     /locus_tag="ACHKUN_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO6658375.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40884..41018)
                     /locus_tag="ACHKUN_000045"
     CDS             complement(40884..41018)
                     /locus_tag="ACHKUN_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658376.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            41094..43655
                     /gene="mutS"
                     /locus_tag="ACHKUN_000046"
     CDS             41094..43655
                     /gene="mutS"
                     /locus_tag="ACHKUN_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO6658377.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43761..44417
                     /gene="pphB"
                     /locus_tag="ACHKUN_000047"
     CDS             43761..44417
                     /gene="pphB"
                     /locus_tag="ACHKUN_000047"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6658378.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(44468..45235)
                     /gene="ygbI"
                     /locus_tag="ACHKUN_000048"
     CDS             complement(44468..45235)
                     /gene="ygbI"
                     /locus_tag="ACHKUN_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO6658379.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            45431..46339
                     /gene="ltnD"
                     /locus_tag="ACHKUN_000049"
     CDS             45431..46339
                     /gene="ltnD"
                     /locus_tag="ACHKUN_000049"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO6658380.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            46336..47598
                     /gene="otnK"
                     /locus_tag="ACHKUN_000050"
     CDS             46336..47598
                     /gene="otnK"
                     /locus_tag="ACHKUN_000050"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO6658381.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            47595..48233
                     /gene="ygbL"
                     /locus_tag="ACHKUN_000051"
     CDS             47595..48233
                     /gene="ygbL"
                     /locus_tag="ACHKUN_000051"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO6658382.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            48238..49014
                     /gene="ygbM"
                     /locus_tag="ACHKUN_000052"
     CDS             48238..49014
                     /gene="ygbM"
                     /locus_tag="ACHKUN_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO6658383.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            49103..50467
                     /gene="ygbN"
                     /locus_tag="ACHKUN_000053"
     CDS             49103..50467
                     /gene="ygbN"
                     /locus_tag="ACHKUN_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO6658384.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(50506..50742)
                     /locus_tag="ACHKUN_000054"
     CDS             complement(50506..50742)
                     /locus_tag="ACHKUN_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO6658385.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(50753..52180)
                     /locus_tag="ACHKUN_000055"
     CDS             complement(50753..52180)
                     /locus_tag="ACHKUN_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO6658386.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(52180..52773)
                     /locus_tag="ACHKUN_000056"
     CDS             complement(52180..52773)
                     /locus_tag="ACHKUN_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO6658387.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            52920..53327
                     /locus_tag="ACHKUN_000057"
     CDS             52920..53327
                     /locus_tag="ACHKUN_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6658388.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(53448..54440)
                     /gene="rpoS"
                     /locus_tag="ACHKUN_000058"
     CDS             complement(53448..54440)
                     /gene="rpoS"
                     /locus_tag="ACHKUN_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO6658389.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(54503..55597)
                     /gene="nlpD"
                     /locus_tag="ACHKUN_000059"
     CDS             complement(54503..55597)
                     /gene="nlpD"
                     /locus_tag="ACHKUN_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO6658390.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(55782..56408)
                     /gene="pcm"
                     /locus_tag="ACHKUN_000060"
     CDS             complement(55782..56408)
                     /gene="pcm"
                     /locus_tag="ACHKUN_000060"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO6658391.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(56402..57163)
                     /gene="surE"
                     /locus_tag="ACHKUN_000061"
     CDS             complement(56402..57163)
                     /gene="surE"
                     /locus_tag="ACHKUN_000061"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO6658392.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(57144..58193)
                     /gene="truD"
                     /locus_tag="ACHKUN_000062"
     CDS             complement(57144..58193)
                     /gene="truD"
                     /locus_tag="ACHKUN_000062"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO6658393.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(58190..58669)
                     /gene="ispF"
                     /locus_tag="ACHKUN_000063"
     CDS             complement(58190..58669)
                     /gene="ispF"
                     /locus_tag="ACHKUN_000063"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO6658394.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(58669..59379)
                     /gene="ispD"
                     /locus_tag="ACHKUN_000064"
     CDS             complement(58669..59379)
                     /gene="ispD"
                     /locus_tag="ACHKUN_000064"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO6658395.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(59398..59709)
                     /gene="ftsB"
                     /locus_tag="ACHKUN_000065"
     CDS             complement(59398..59709)
                     /gene="ftsB"
                     /locus_tag="ACHKUN_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO6658396.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(59903..60226)
                     /gene="ygbE"
                     /locus_tag="ACHKUN_000066"
     CDS             complement(59903..60226)
                     /gene="ygbE"
                     /locus_tag="ACHKUN_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO6658397.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(60276..60881)
                     /gene="cysC"
                     /locus_tag="ACHKUN_000067"
     CDS             complement(60276..60881)
                     /gene="cysC"
                     /locus_tag="ACHKUN_000067"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO6658398.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDTDRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(60881..62308)
                     /gene="cysN"
                     /locus_tag="ACHKUN_000068"
     CDS             complement(60881..62308)
                     /gene="cysN"
                     /locus_tag="ACHKUN_000068"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO6658399.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(62310..63218)
                     /gene="cysD"
                     /locus_tag="ACHKUN_000069"
     CDS             complement(62310..63218)
                     /gene="cysD"
                     /locus_tag="ACHKUN_000069"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO6658400.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            63470..64507
                     /gene="iap"
                     /locus_tag="ACHKUN_000070"
     CDS             63470..64507
                     /gene="iap"
                     /locus_tag="ACHKUN_000070"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO6658401.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   64589..64922
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=64650..64678
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(65019..65312)
                     /gene="cas2e"
                     /locus_tag="ACHKUN_000071"
     CDS             complement(65019..65312)
                     /gene="cas2e"
                     /locus_tag="ACHKUN_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO6658402.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(65312..66232)
                     /gene="cas1e"
                     /locus_tag="ACHKUN_000072"
     CDS             complement(65312..66232)
                     /gene="cas1e"
                     /locus_tag="ACHKUN_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO6658403.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(66229..66879)
                     /gene="cas6e"
                     /locus_tag="ACHKUN_000073"
     CDS             complement(66229..66879)
                     /gene="cas6e"
                     /locus_tag="ACHKUN_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO6658404.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(66861..67607)
                     /gene="cas5e"
                     /locus_tag="ACHKUN_000074"
     CDS             complement(66861..67607)
                     /gene="cas5e"
                     /locus_tag="ACHKUN_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO6658405.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(67618..68673)
                     /gene="cas7e"
                     /locus_tag="ACHKUN_000075"
     CDS             complement(67618..68673)
                     /gene="cas7e"
                     /locus_tag="ACHKUN_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO6658406.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(68685..69224)
                     /gene="casB"
                     /locus_tag="ACHKUN_000076"
                     /gene_synonym="cse2"
     CDS             complement(68685..69224)
                     /gene="casB"
                     /locus_tag="ACHKUN_000076"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO6658407.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(69221..70783)
                     /gene="casA"
                     /locus_tag="ACHKUN_000077"
                     /gene_synonym="cse1"
     CDS             complement(69221..70783)
                     /gene="casA"
                     /locus_tag="ACHKUN_000077"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO6658408.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(70881..>73529)
                     /gene="cas3"
                     /locus_tag="ACHKUN_000078"
     CDS             complement(70881..>73529)
                     /gene="cas3"
                     /locus_tag="ACHKUN_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO6658409.1"
                     /translation="FDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLA
                     DYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISH
                     STLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELDNFL
                     PEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTR
                     FFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKV
                     LDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYD
                     RLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAW
                     FADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCI
                     LEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHV
                     TKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKV
                     HRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIE
                     QSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVW
                     DDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVV
                     MPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLW
                     LATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPE
                     MANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT        17323 a        20128 c        18855 g        17223 t
ORIGIN      
        1 aagtaagaga tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt
       61 gctaaagcaa cgttatcatc cctggtgcct gcggttaatt ccgctacctc ttcgatacct
      121 tcattgctga agatttcgga gaatatggtg catccgggag gattcgaacc tccgaccgct
      181 cggttcgtag ccgagtactc tatccagctg agctacggat gcatcgggaa aatttatttt
      241 actgctgata ttgataccgc tactaaagcc atatcaagta agagatggtg catccgggag
      301 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
      361 gcatcgggaa acttacttta ctgcagattt tttgataccg ctactaaagc cgtatcaagt
      421 aagagatggt gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta
      481 aagcaacgtt atcctccctg gtgcctgcgg ttataccgcc acctcttcca taccttcatc
      541 gctgaaagta cgtagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
      601 gtagccgagt actctatcca gctgagctac ggatgcaaat ggcggtgagg cggggattcg
      661 aaccccggat gcagcttttg accgcatact cccttagcag gggagcgcct tcagcctctc
      721 ggccacctca ccacacgcct cttacgagtg cttcgaagaa cttgtttatc gctcatcgtc
      781 gctgcgtggc gcacatatta ctttctgaga cttataagtc aaacaatttt tcctacactt
      841 ttatcgtttg cacacttcac gttcaattag tctgtaaaaa cgtcaaaaag agtgttttat
      901 caacagaaaa atggaggtct gacagatagt agtaatgcaa aaaaatggag acttaagttg
      961 aatgaacggg agtaaagcga aaagactata gagtgaagga gaaattttga gggtgcgtct
     1021 caccgataaa gatgagacgc ggaaagatta gtaactggac tgctgggatt tttcagcctg
     1081 gatacgctgg tagatctctt cacggtgaac agaaacttcc ttcggggcat ttacgccaat
     1141 acgtacctgg ttgcccttta cccctaaaac tgtcacggtg acctcatccc caatcatgag
     1201 ggtctcacca actcgacgag tcagaatcag cattctttgc tccttgaaag attaaaagag
     1261 tcgggtctct ctgtatcccg gcattatcca tcatataacg ccaaaaagta agcaatgaca
     1321 aacacattac atgtaagcag tcatggcatt acattctgtt aaacctaagt ttagccgata
     1381 tacacaactt caacctgact ttatcgttgt cgatagcgtt aatgcgaatg ccgtgaagcg
     1441 agtccacggc attgcctgac gcttatatta ttgcaatttc gcgctgaccc agcctttcac
     1501 actggctaac gctgcaggta aggccgcagc atccgtacca ccggcttgcg ccatgtcagg
     1561 acgtccacca cccttgccgc ccacctgctg agcgaccata ccaatcagtt cccctgcttt
     1621 cacacgatct gtgacgtcct tagatacgcc tgcaatcaga gaaaccttac cttcggctac
     1681 cgttgccagc acgataattg tcgaccccag ctgatttttt aaatcgtcaa ccatggtacg
     1741 caacattttc ggctcaacac cgctaagctc gctaaccaac agcttaacac cattaacatc
     1801 aattgcctta ctggaaagat ttgcgctctc ctgtgcggca gcttgttctt taagctgttg
     1861 tagttctttt tctaactgac gcgtacgttc cagtactgag cgcactttat ccgccagatt
     1921 attgctatcg cctttcagca gatgcgcgac ttcgcttaag cgatcgctgt ctgcatgaac
     1981 ggtggtgata gcgccttctc cggttaccgc ttcgatacga cgaacgcctg cagcagtacc
     2041 cgattcagag atgatgcgga acagaccaat atcaccagtg cggctggcgt gagtaccgcc
     2101 acacagctcg gtggagaaat cgcccatgct cagcacgcgt acacgctcat catacttctc
     2161 gccgaacagc gccatcgcac ctttcgcttt cgccgcttcg agatccatga tgttggtttc
     2221 gatcggcaaa ttgcgacgaa tctgtgcgtt caccaggtct tcgaccgcac gaatctcttc
     2281 cggtttcatc gcttcgttgt gtgagaagtc gaagcgcagc accttgtcgt taaccagtga
     2341 acctttctgc gatacatgag tccccagaac ctggcgcagc gcagcgtgca tcaggtgcgt
     2401 tgcggagtga ttcagacgaa tacgggcgcg acgagcctca tcaacatcag cctgcacagc
     2461 gtcgcccact ttcagagaac ccgcagcaag tttaccgatg tgaccaatcg cctggccgta
     2521 tttctgcgta tcttccaccg cgaaggagaa gttagcgcct ttcagttcgc ctttatcacc
     2581 aacctgaccg ccggattccg catagaatgg tgtttgatcc agcacgacca cagcttcctg
     2641 gcctgcattg attgcatcaa ccgctttacc atcaacaaac agcgcggtca ctttgccgtt
     2701 cagttccaga tggtcatagc ctttaaattc agatgcacta tcaacacgga tcattgcgtt
     2761 gtaatcggca ccaaagccgc tggcttcgcg cgcgcgacga cgctgctctt ccattgctgc
     2821 ttcaaaacca gcttcgtcaa ctttgatgtt gcgctcacga caaacatcag ccgtcaggtc
     2881 aaccgggaag ccataggtgt cgtacagacg gaaagccgtt tcaccatcca gcgtatcacc
     2941 agaaagtttt gccagctctt catccagcaa cgccagaccg cgctccagag tacgggcaaa
     3001 ctgctcttct tcagtcttca gcacctgctc aacctgcgcc tgctggcgtt tcaggtcttc
     3061 acccgcagag cccataacgt cgatcagcgg accaaccagt ttgtagaaga aggtttcttt
     3121 cgcgccgagc atattgccgt gacgcactgc gcgacgaatg atacgacgca gtacataacc
     3181 acggttttca ttggacggca ttacgccatc cgcgatcagg aacgcacaag aacgaatgtg
     3241 gtcagcgatt acgcgcagcg atttattgct cagatcggtc gcgccagtga ctttcgctac
     3301 cgcctggatc aacgtgcgga acaggtcgat gtcatagtta gagttaacgt gttgcagcac
     3361 cgcagcaata cgctccagac ccataccggt atctacagac ggcttcggca gcggttccat
     3421 cgtgccatct gcctggcggt tgaactgcat gaagacgatg ttccagatct caatgtagcg
     3481 gtcgccgtct tcttccggac ttcccggagg accaccccaa atgtggtcgc cgtgatcgta
     3541 gaagatttcg gtgcacgggc cgcacggacc agtgtcaccc atctgccaga agttgtcaga
     3601 tgcgtatggc gcacctttgt tatcgccgat gcgaataata cgttcgcgcg ggatcccgac
     3661 ttctttttcc cagatttcgt aggcttcgtc gtcgctttca tagacggtaa cccacagacg
     3721 ctctttcggc agggcaaacc atttttcgct ggtcagcagt tcccatgcaa actgaatggc
     3781 atcgtgtttg aaatagtcgc cgaagctgaa gttgcccagc atttcgaaga aggtatggtg
     3841 acgcgcggtg taaccgacgt tttccaggtc gttgtgttta ccacccgcac gcacgcagcg
     3901 ttgggaagtg gtagcgcggg aataattacg cttgtcgagc ccaaggaaca catccttgaa
     3961 ctggttcatc ccggcgttgg taaacaacaa agttgggtcg ttatggggta ccagggagct
     4021 gctggcaact acctgatgtc ccttactatg gaaaaagtcg agaaacgcct gacggatctc
     4081 agcggtgctc ttgctcataa ttatcctgaa atcaagctaa cgaaatatcg ccaccagctc
     4141 cagcgtgtct taaccgccgg gctggtaact gaaaagtggg aataagataa gttttcttga
     4201 ctgggaagta aaataccgta tgcgttcagt cggcaaaatt tcgccaaata tcctggatat
     4261 cttccatcag atagccacga tagagcagaa aacgctggat cttaactttt tctgaaaaga
     4321 cagttggcaa aggttcacca tattttcgcg tcgcctgatc gcgcgccagt gcgcaccagt
     4381 cgatgtcaca ttcacgcatc gctttttctg tcgcttcgcg ggaaatacct ttctgattca
     4441 gttcctgacg aatacgtgca ggtccataac ctttgcggct acggctggcg ataaagcgcg
     4501 caacaaatcg gctgtcatcg agatagccat gttcatggca ccaggcaata acgcgctcgt
     4561 aatcttctgc cgtagcatca atctcttctg ggccattttt gcccataatc ggtgccacga
     4621 gtttacgtcg cagttcttgc tcactgtgat cgcgcaccgc cagaatgcgt accgcacgat
     4681 ccaacaggcg agcatatgcc gggcgacggg acgttgattc tgtcatggca tatccttaca
     4741 acttaaaaaa gcaaaagggc cgcagatgcg acccaggttt tgctgataaa cagatttaat
     4801 agtcaatgag tgtgcagaat gttcccgcag gcccaattcg ttagaggatc gaggtgagag
     4861 taatttttca cgccagtgct ggcctgttcc cctcccccta accctctccc caaaggggcg
     4921 aggggaccgt ccgtgcacac attatctatt atcatcaata acgaacgatt aaaaatcttc
     4981 gttagtttct gctacgcctt cgctgtcatc tacagagaaa tccggcgttg agttcgggtt
     5041 gctcagcagc aactcacgta ctttcttctc gatctctttc gcggtttccg ggttatcttt
     5101 cagccaggca gttgcattcg ctttaccctg accgatcttc tcacctttgt agctgtacca
     5161 cgcgcctgct ttctcgatca gcttctcttt tacgcccagg tcaaccagtt cgccgtagaa
     5221 gttgatacct tcgccgtaga ggatctgaaa ttcagcctgt ttaaacggtg cagcgatttt
     5281 gttcttcacc actttcacgc gggtttcgct acccaccacg ttttcgccct ctttcaccgc
     5341 gccgatacga cggatgtcga gacgaacaga ggcgtagaat ttcagcgcgt taccaccggt
     5401 agtggtttcc gggttaccga acatcacacc aattttcata cggatctggt tgatgaagat
     5461 cagcagcgtg ttggactgct tcaggttacc cgccagctta cgcatcgcct ggctcatcat
     5521 acgtgccgca aggcccatgt gagagtcgcc gatttcgcct tcgatttccg ctttcggcgt
     5581 cagtgccgcc acggagtcaa cgacgataac gtctactgcg ccagaacgcg ccagggcgtc
     5641 acagatttcc agtgcctgct cgccggtgtc cggctgggag cacagcaggt tatcgatatc
     5701 gacgcccagt ttacgtgcgt agattgggtc cagcgcgtgt tcagcatcga taaacgcaca
     5761 ggttttacct tcacgctgcg ctgcggcgat cacctgcagc gtcagcgtgg ttttaccgga
     5821 agattccggt ccgtagattt cgacgatacg gcccatcggc agaccacctg ccccaagcgc
     5881 gatatccagt gaaagcgaac cggtagagat ggtttccaca tccatggaac ggtcttcacc
     5941 caggcgcatg atggagcctt taccaaattg tttctcaatc tggcccagtg ctgccgccaa
     6001 cgctttctgt ttgttttcgt cgatagccat ttttactcct gtcatgccgg gtaataccgg
     6061 atagtcaata tgttctgttg aagcaattat actgtatgct catacagtat caagtgtttt
     6121 gtagaaattg ttgccacaag gtctgcaatg catacgcagt agcctgacga cgcaccgcat
     6181 cacggtcacc gctgaagcat tcccgccggg taatgccttc accgcgggca gtggcaaaag
     6241 caaaccagac ggtgccgaca ggcttctctt cactgccgcc atccggcccg gcgataccac
     6301 taatagacac ggcataatca gcacgagccg ctttcagtgc acctatcgcc atttccacca
     6361 cgacgggttc actcaccgcg ccatgctgcg ccagcgtctc ttcgcgtacg ccgatcatct
     6421 gcgctttggc ttcgttactg taggtgacaa atccgcgttc aaaccaggcg gagctaccgg
     6481 caatatcggt aatcactttc gctacccaac caccggtaca agactcggca gttgttacgg
     6541 ttgcgccacg ggctttcagc gcctgcccaa cctgttcact taactgcatc agttcactgt
     6601 cagtcatcac aacctctgtc agtcaaaaat ttatgccgga caagatagca ctttcgtcag
     6661 gaagatggtg ttggcgttgc aattttacga ggaggattca gaaaaaagct gattagccag
     6721 agggaagctc acgcccccct cttgcaaata gttactgcac gcgcgccagc gccacggctt
     6781 gtcctaactg ccagaccgcc attgcgtaat gggtgctgtg gttataacgg gtaatggtgt
     6841 agaagttcgg cagaccgtac cagtactggt aaccggtacc gacatccaga cgcagcaggc
     6901 tggcttgttg atggttgccc agcggctgct gtggcgttaa acctgccgtg gcaagctgcg
     6961 aaatgctgta cctggttttg aagccatttg gcaagcctgg agcctgaccg tttgccatta
     7021 ccgcgacctg atcgcctttc acccagccgt gcgctttgaa atagttcgcc acgctaccga
     7081 tcgcatcaac cggatcccac aggttgatat gcccgtcgcc gctgaaatct accgcatatt
     7141 gtttgtaaga cgacggcata aactgtacgt agcccatcgc cccggcaaag gaacctttca
     7201 gattgagcgg atcgtcctgc tcgtcgcgcg ccatcaacag gaaggtttcc agctcgccag
     7261 agaaatactc cgcgcggcgt gggtagttaa atgacagcgt tgccagcgca tcgaggatgc
     7321 gagttttccc catcacgcgc ccccagcggg tttcaacgcc gataatcccg acgataattt
     7381 ccggcggtac gccatacacc tgccacgcgc gattcaacgc atcttcatac tgattccaga
     7441 aaaccacacc gttctgcacg ttgtccggcg taataaattt tttgcgataa cggagccatg
     7501 cgccgttcgg acctgatggc ggtttcaccg atgtggttgg tgcctggtta tccatcagcc
     7561 gcagtaccga atccagacgc ttcgcctggg agagaatttc ctgcaactgc tgacgatcga
     7621 aaccgtgttt gttcaccatt ttgtcgatga actgctgggc attcgggtta ttagcgaaat
     7681 cgccgcccat ctgcatcaca ttgtgctgcg gctcaagcag gaagccgcca gacggcgttc
     7741 cggtggtcgt ttcagtctca gtaggttttg gcttgctgct acaggcggca agcaacacaa
     7801 aaaggggaag caatgttaca taacgacgct tgaacatgag gggtccattt aacagattca
     7861 accaggggca agtatggtaa agcatcacgc cccacacaag gaagcggtag tcactgcccg
     7921 atacggactt tacataactc aactcattcc cctcgctatc cttttactca aactttcaaa
     7981 ttaaaatatt tatctttcat tttgagatca aaataacact tttaaatctt tcaatctgat
     8041 tagattaggt tgccgtttgg taataaaaca ataaatcctg aaggagagaa caatgataga
     8101 aaccattact catggtgcag agtggtttat cgggctgttc caaaagggcg gagaggtgtt
     8161 taccgggatg gtgaccggca ttcttccgct gttgattagc ctgctggtta tcatgaacgc
     8221 actgattaat tttatcggtc agcatcgtat tgaacgtttt gctcaacgtt gcgccggtaa
     8281 ccctgtttcc cgttacctac tgttaccgtg cattggcacg tttgtctttt gcaatccgat
     8341 gaccttaagc ctgggtcgct ttatgccgga aaagtacaaa cccagctact acgcggcggc
     8401 ctcttatagc tgccactcaa tgaatggcct cttcccccat atcaaccctg gcgaactgtt
     8461 tgtttatctt ggcattgcca gcggtctgac aacgctgaac ctgccacttg gcccactggc
     8521 ggtgagttat ctgctggttg gtctggtcac caatttcttc cgcggctggg tgaccgatct
     8581 gaccaccgcc atttttgaga aaaagatggg cattcaactt gaacaaaaag ttcacctggc
     8641 aggagcaaca tcatgacgca tattcggatc gaaaaaggaa cgggtggctg gggcggcccg
     8701 cttgagctga aagccacgcc gggaaaaaaa atcgtctata tcaccgccgg tacccggcct
     8761 gcgattgttg acaaactggc acagcttact ggctggcagg ctattgacgg atttagagaa
     8821 ggtgaacccg cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc
     8881 tgcggcatct atccgaaacg acgtattccc accattaata tccactcgac gggcaagtcc
     8941 ggtccgctgg cgcagtacat tgtggaagat atttatgtct ctggcgtaaa agaagaaaac
     9001 atcactgtag tgggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc
     9061 agtaagaaaa tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggcatgggg
     9121 tccgccgttg cggtgctgtt tcaatctggt cgtgacacca tcgacactgt attaaaaacc
     9181 attctgccgt ttatggcatt cgtctcggcg ctcattggca tcattatggc ttctggcctt
     9241 ggtgactgga ttgcccacgg tcttgctccg ctggcgagcc atccactggg tctggtcatg
     9301 ctggcgctca tctgctcctt cccactgctt tcacctttcc tcggcccagg cgcagttatc
     9361 gcacaggtta tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat
     9421 ctggctttac cggcactgtt tgccatcaac gcgcaggcgg cctgcgactt catcccggtc
     9481 ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgtactg
     9541 gtgagccgct ttttaaccgg cgcaccgact gtactgatcg cctggtttgt ctccggtttt
     9601 atctatcaat agaggctgaa acatgaccgt tatttatcag accaccatca cccgtatcgg
     9661 cgcgagtgcc actgacgccc tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc
     9721 agcggacctc gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca
     9781 tcccggtttg caatttacgc tcgggcagca tcgctatccg gtgaccgctg ttggcagcgt
     9841 ggcggaagac aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga
     9901 agcggaattt ccgggcactg tccatgtggc aggccctgta cccgacgata tcgcgccggg
     9961 atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat
    10021 cggtggtggg caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg
    10081 cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc
    10141 cgaatatggt gaaagtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt
    10201 tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctatag
    10261 cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc
    10321 gctacaagtg aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat
    10381 ccgcgacggg attcagggtc gcattattca gatcaactcg aaatccggca aagtgggcag
    10441 caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact
    10501 ggcgctggat ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaacctgct
    10561 gaaatcgccg atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc
    10621 ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca
    10681 agatgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca
    10741 gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca acagcggaga tccattaagg
    10801 atctccgtga gactatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg
    10861 ccactacatt aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctgg
    10921 tgtgcgcaac tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca
    10981 ctatgccagc aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa accgcgggtc
    11041 gtggtcgcca tcgcgctgga cgatcagcag cgcatcgtcg acaccttgtt tatgaaagga
    11101 ctgaccgtct tcgcccgacc gcaaaaaatt cccgcaatta ccggtatgca tgcgggtgat
    11161 ttacagcccg atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg
    11221 cttaaactga aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt
    11281 tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg
    11341 gagtatctgc aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc
    11401 acaggcacaa ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt
    11461 acttatggcg gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaaca
    11521 ctcatcaaca cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat
    11581 ggcgattcga tcattcttga tgctggcagt accgttttgc agatggttcc cctgctctcg
    11641 cgctttaata acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa
    11701 ctggataacg aacaaactat cctgatgcca ggcggaacgt ttcgtaaaaa atcggcctca
    11761 tttcacggac agctggcaga gaatgccttc gaacatttca gtttcgataa attgtttatg
    11821 ggcaccgacg gcatcgatct caatgcgggc gtaaccacct tcaacgaggt ttataccgtc
    11881 agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt
    11941 ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg tcgataagct gattaccgac
    12001 gcaggtatcg atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata
    12061 accggagaga gcaatgagtg aagcactact gaacgcggga cgtcagacgt taatgctgga
    12121 gttgcaggaa gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa
    12181 tatcatcctg cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat
    12241 tggtaagaaa atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc
    12301 ggcagaagca ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat
    12361 ctcttactcc ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc
    12421 tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcaaaagc
    12481 ggtgctggat atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag
    12541 caccgtcaat accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg
    12601 atttaatgaa gaagattttg cccgctccca cccagccggg gcactgggcg ctcgcttgct
    12661 gaataaagtg catcatctga tgcgccgtga cgatgccatc ccacaggtgg cgttaaccgc
    12721 cagcgtgatg gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg
    12781 tgacgctcaa caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt
    12841 tggcggcggc gcactcacca cgccagttaa tgaagcgatg acgacaggcg gcaccacatt
    12901 acaggcacaa agtcgcgcca tcgacgccaa agagatcctg atgaagcgca aaatcactgc
    12961 cgcaccggtg gtggatgaaa acggcaaact caccggcgca attaacctgc aggatttcta
    13021 tcaggccggg attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc
    13081 gacgtcggtt tccagcatcc gcgcgcaggc agcccagttg tgatgatttt gtgccagtgc
    13141 ctggcgaatg gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac
    13201 gggcaccgcc gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgtgc
    13261 ctcaagaatc acttcatcgc cgctgcgagt ggctctcgcc agcactaccg cccgatgaat
    13321 agcatgttcc agttcgcgca cgttccccgg aaaacgatag tgttgcagta aatttcgcgc
    13381 tccggcactt aataccacgc gggagagccc cagccgcaaa cgacactgct cgcagaaata
    13441 ccccgccagc agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa
    13501 cacgctcagg cgatgaaaca aatcggcgcg gaatcgccct gccagcacct cttcgcgtaa
    13561 gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac
    13621 gcgctgaata tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa
    13681 ctcgccgatc tcatcgagaa acagagtgcc gttatccgcc atttcaaact tcccgctgcg
    13741 gttactgata gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac
    13801 actttccggc agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc
    13861 ttcatgaatc gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcagaac
    13921 gttgagatcg gacgccgcca caatctcaat ctcttttttc aattgcgtca tgcctggcga
    13981 caagccaatc atctgcgtct gtttcaccgc ttcaaacggc gcggcatcgc ccggcagcat
    14041 attctggctt tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc
    14101 ggcaatcagc cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc
    14161 gagcgtcagt gcgccgatca ggttttgccc ggcaaacaat ggcagaccaa cgcaggcgtg
    14221 aaccttcaga ctctcctgcc caggaatcaa accgtcatag ggatcgggca attcgctgtc
    14281 tgcaggaaag cgcaccacgt ccccggcgcg ggcaatcgct tcaagccgtg gatgcccttc
    14341 cagcgcaaag cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa
    14401 ctgccgcgaa tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt
    14461 ggtgatcagg cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat
    14521 attcgccagc acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat
    14581 attgtcatat cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt
    14641 tatttataga gtaaaaacaa tcagataaaa aactggcacg caatctgcaa ttagcaagac
    14701 atctttttag aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac
    14761 attcattggg ttggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa
    14821 acgctgcgcg gcagcagtta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc
    14881 gacaccgtcg accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat
    14941 ctggcggata tcgattacat cgtgattaac catgcagaag aggaccacgc tggggcgctg
    15001 accgaactga tggcgcaaat tcccgatacg ccgatctact gtactgccaa cgctatcgac
    15061 tcgataaatg gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac
    15121 acgctggata tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg
    15181 ccggacagca tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc
    15241 ggtcagcact actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc
    15301 gagcagtgcc agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg
    15361 aaaattaccg agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc
    15421 gtggtctggc gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat
    15481 tatcaggaag acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg
    15541 gctgacgcta tcgcccaggg aattgcggaa accgacccac gcgtggcggt gaaaattttc
    15601 aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg
    15661 ctggtcggca cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag
    15721 gagatgactg gtttacgctt ccgtaacaaa cgcgccagtg ctttcggctc tcacggctgg
    15781 agcggcggtg cggtggatcg tctttccacg cgcctgcagg atgcgggttt cgaaatgtcg
    15841 cttagcctga aagccaaatg gcgaccagac caggacgctc tggagttatg tcgtgaacac
    15901 ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg
    15961 gtggttaaag aagaaacctc tgccaccacg acggctgacc tcggcccacg gatgcagtgc
    16021 agcgtctgcc agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca
    16081 ggaacgccgt ggagtgaagt tccggataac ttcctctgcc cggaatgctc cctcggcaaa
    16141 gacgtctttg aagaactggc atcggaggca aaatgagtaa cggcattgtg atcattggtt
    16201 cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat
    16261 taaccctgat tgccgccgac agcatggatg agtacaacaa acctgacctc agccatgtta
    16321 tcagtcaggg gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgagc
    16381 agtttaatct gcgcctgttt ccgcacacct gggtgacgga tatcgatgcc gaagcccgtg
    16441 tggtgaaaag ccagaataat cagtggcaat acgacaagct agtgctggca accggtgcca
    16501 gcgcctttgt cccgccagtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag
    16561 agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gtcggtggtg
    16621 gcttgattgg tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa
    16681 tcgacaacgc tgccagtatt ctggcgtcat taatgccacc ggaagtaagc agccgcttgc
    16741 agcatcggtt gacggagatg ggcgttcatc tgttgttgaa atctcagttg caggggctgg
    16801 aaaaaacaga ttctggcatt ctggcaacgc tggaccgcca gcgcagtatc gaagtggatg
    16861 cggtaattgc cgccaccgga ctgcgcccgg aaaccgccct ggcacgacgc gccgggctga
    16921 cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg
    16981 cgctgggcga ttgcgcggaa attaacggtc aggtattgcc gttcctccag ccgattcaac
    17041 ttagcgcgat ggtgctggca aaaaatcttc tcggcaataa cacgccactg aaactaccgg
    17101 cgatgctggt gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc
    17161 gtcaggattt acgctggcag attaataccg aacgccaggg aatggtggcg cgcggcgttg
    17221 acgatgctga ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg
    17281 gattgttgaa aacgttgccg atgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg
    17341 ctggcgcgtc ttatccaacc tacggggagc gcatgtgttg gtcacatact gcgttcatgt
    17401 cgcatccacc tactgtgccc aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta
    17461 cggggacgca tgtgtaggcc ggataaggcg tttacgccgc atccggcaat ggtgtccaaa
    17521 tgcaacacgt tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc
    17581 ccctgcccca gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg
    17641 aaatcagcga gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg
    17701 ctaaatacca gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa
    17761 ccctgcgcca gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc
    17821 agccactgct gccagaaagt ggcgagatcc agttgattgt ccaccagcgg cattgtcacc
    17881 ggatgcgtca ctccgtggca cgaagctgcg agtgcctcca gagcacaagc cgcttcacct
    17941 tcataactta acgtggctgg cgcacagccc agtgccgccg ccacggcatc gaacaaccgc
    18001 ccacacgatg acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagcacgctc
    18061 cagttttgct gttgcacact tgctgtttca gaataattct gccactccgg cacaaagcgc
    18121 aggcactgcg ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc
    18181 gccactgcag gcaagccgcc caggtgctca cattcgcgat agttcacccg cagacactcg
    18241 ccgccccaca aagcgccgtt ctcccccata ccgataccat cgagcgtcaa agcaatgaca
    18301 tccccgccat ccagcggcca ctgatgctct gccagacacg ccgctgcgtg ggcatgatga
    18361 tgcagcaccg tttgcgtcgg catattcatt tcgctcgccc actggctgga gacatagccc
    18421 ggatgcacgt catgcacaac gtattgcgga gtgaaatcgt agatgttttg catcaggcgt
    18481 aacgcttcgc gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc
    18541 aacaccgctt gttcaccgcg caccaggcag aaggtgtttt tcagatccgc gccgagacac
    18601 agcacaggcg gaacattttt aaagcccgga ggcaaagcca gcgcatccgg cacatacccc
    18661 cgcgaacggc gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg
    18721 atgtcgcggt tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt
    18781 tcgttgctga tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat
    18841 tgcagttcct gtaacagcaa atgctggagc gggttcgcag gcaacattac cccgacttcg
    18901 ttaaggtcag gggcgatatc atcacaaagc tcaggaacgt attttttatc caccagcaca
    18961 atcggcgcgg cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaacc gtcagccact
    19021 ggcaacatga ctgccagcgg tttcgccggg cggtgtttgc gctcccgcag tgtcgccacc
    19081 gcgttactgt tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca
    19141 attttgccca tttttaactg tgcgatagct gcctgtaatg ccgcctcttg ttccgcatgt
    19201 tcaccatgac ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg
    19261 tggaagcgac gatcgagcgg gtcgcggtac tctttatcac aggctggaca cagcggaaac
    19321 tccgccatta cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg
    19381 ccgcagtggg tacagttgat aaacggataa cgataacgcc gttcgcctgg ggtattcatt
    19441 tcggcaaggc aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct
    19501 cccgcgctct ggcggatagt gaactcggtg ggcagttgtg accagataaa cggctcacgc
    19561 tcgacgctat caatacgcgc cagtggcgga cagtgctgat gcaattgaac aagaaacgtt
    19621 tccgggtctt ccagcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga
    19681 agatttaatt gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg
    19741 ccacgaatac gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca
    19801 tcatgaattg cgtaacccgt ccagccggac acgacagcgt cgcatccggc agtcacaggt
    19861 cggcgatacc gcccgcttcg tattctacga atatttccgg gaattccttt gatgccagaa
    19921 cagttctgta agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt
    19981 gttcaagttt attacgatcg acacaaatta gcgcatgggt cgggcaagcc gccatacacg
    20041 ccgggccgtc ttcacgatgg ttgcacaggt cgcatttatt ggcttcagct ttgtcagccc
    20101 gtacattcag ccccacgccg ctgttgcgaa tcaccggacg taccaccact tccatcgcgc
    20161 catacgggca agccaccacg caggttttgc aaccaataca acgttcctgc atcacatgaa
    20221 caaacccttt atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt
    20281 cacactgacg gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg
    20341 gtaaaaatgt ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc
    20401 acgccacttc acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt
    20461 tcatcaaatt ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt
    20521 tttaatttac tgttatttaa ggaaattaat ttctgtaatg caggaaaaac gatgtcatcg
    20581 acactagtga cgatgacatg tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg
    20641 cggcagatca attttccgct gaaggttttc ggcggtgaga aatccccgcc atcgagcata
    20701 aaaatcagcc gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag
    20761 agcgccggaa cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc
    20821 tctggcaccg ctacgccgcg ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg
    20881 ttactggcaa ctaacgcgct aaatttagcc ccacgttcga gcaacatttc taccccttcg
    20941 gccccgctgg caggcgtcca tctaccgtta gcgataagtt tttcattgag cgcaataccg
    21001 tgctgcgcca gcgcgtcttt atatccggca agacgttcaa cgctggtggg agaatccatc
    21061 gaaccggtaa ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcgtta
    21121 aaactggtct gtttatgatc gcaccagacg ctatggctgc tgtttttgcg caggcggcga
    21181 ttaagcacca ttatcggctg actgtgcgcg tcaatgatgt catcgatctc atccacgctt
    21241 aaaaaacgcg ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc
    21301 tggcgctctt cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct
    21361 tccgccattc gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg gtaaagcgtg
    21421 ttggtcacta ccagccccag cgtctgagta ctcttcgccg acaaattgcg cgccagcaag
    21481 tttgggcggt aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg
    21541 acgtagccat tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc
    21601 gccacttcca gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac
    21661 tgcaccgttt gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt
    21721 cacaaatata aaaaatgaac aattaactct cttgcttatt tagtgacaac tattcatgat
    21781 tttgtgaaac cggtttccta attccgtttc agcatcggca tttttccgtt acgtcgactg
    21841 ataacaacta catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg
    21901 gcgctggcac gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcggtcacg
    21961 cactgtatga cgcgtttgcg ctttgttatc aaagatgacc agcttatcga cagcccgacg
    22021 ttaaaaacca tccccggcgt gctcggcgtg gtacgtagtg acaaccagtg tcaggtgatt
    22081 atcggcaata ccgtttcaca agcctttcag gaagtcgtca gcctactgcc gggagatatg
    22141 cagcccgcac agcccgtggg taaacccaaa ctcacgctgc gtcgcattgg tgcagggatc
    22201 ctcgatgcgc tgatcggcac catgtcaccg ctgatcccgg cgattatcgg cggatcgatg
    22261 gtcaaactgc tggcgatgat cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc
    22321 ttaaccattc tgaatgtgat tggtgacggt gctttcttct tcctgccgct gatggtcgcg
    22381 gcatctgccg ccatcaaatt taaaaccaat atgtcgctgg cgattgccat cgcgggtgtg
    22441 ctggtacatc cgagttttat tgaactgatg gcgaaagcgg cccagggtga acatgtcgaa
    22501 tttgccctga ttccggtcac cgcggtgaaa tacacctaca cagtgatccc ggcgctggtg
    22561 atgacctggt gcctgtcata tatcgaacgc tgggtggatc gcattacacc ggcggtgacc
    22621 aaaaacttcc tcaagccaat gctgattgtg ttgattgccg caccgctggc aatcctgctg
    22681 attggcccga ttggtatctg gatcggtagc gccatttcgg cgctggttta caccattcat
    22741 ggttatctgg gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg
    22801 accgggatgc accgcgtctt tacgccaacc atcattcaga ccattgccga aaccggcaaa
    22861 gaagggatgg tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg
    22921 gcggtggcgt ggaaaacgaa aaacccggaa ctgcgccaga cggcgctggc tgcggcggca
    22981 tcagccatta tggcggggat ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa
    23041 cgtccgctta tcgccagtct tatcagcggc tttatttgcg gcgcggttgc cggtatggcg
    23101 gggcttgcca gccactcaat ggcagcgccg gggctattta ccagcgtgca gttcttcgat
    23161 ccggcgaatc caatgagcat cgtctgggtg ttcgcggtca tggcgctggc ggtcatgctg
    23221 tcgtttatcc tcacactgtt gctcggcttt gaggatattc ctgttgagga agcgactgcc
    23281 gaggcgcgaa agcatcagag cgtacaaccg accgtcgcca aagaagtaag tcttaattga
    23341 ggatgaaaat gtcagtattt ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc
    23401 agtctgaagg tgcgttccgt gaaggtggca aagggctgac cactgtcgat atgatcccac
    23461 acggcgagca tcgaatggcg gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg
    23521 acgagtttta tcccagccat gaggcgacgg atttttatca tcgttataaa gaagatatcg
    23581 ccctgatggc agagatggga ttcaaggttt tccgtacctc aattacctgg agccgtctct
    23641 ttccgcaggg cgatgaaatc acgcccaatc agcagggcat tgctttttat cgttctgtct
    23701 ttgaagagtg taaaaagtac ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc
    23761 cgatgcatct ggtcaccgaa tatggctcct ggcgtaaccg caagctggtg gagtttttca
    23821 gccgctacgc ccggacctgc tttgaagcat ttgatggtct ggtgaaatac tggcttacct
    23881 tcaatgaaat caacattatg ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag
    23941 aaggtgaaaa tcaggatcag gtgaaatatc aggccgcgca tcaccagctg gttgccagtg
    24001 cgctagccac caaaatcgcc catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg
    24061 cgggcggtaa cttctaccct tacagttgca agccggaaga tgtctgggcg gcgctggaga
    24121 aagaccggga aaacctgttt tttatcgatg tgcaggcgcg gggcgcgtat ccggcttact
    24181 ctgcccgcgt attccgcgaa aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa
    24241 tcctgaaaaa caccgtcgat tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg
    24301 cggagatgaa cgccaacaat agcagtgcgg cgaacgtagt gaaatcgctg cgtaacccgt
    24361 atctgcaagt gagcgactgg ggctggggta ttgatccact cggtctgcgt atcaccatga
    24421 acatgatgta cgaccgttat cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa
    24481 aagatgaact tgctgccaat ggcgagatta acgatgacta tcgcatcagc tatttacgcg
    24541 aacatatccg cgcaatgggc gaagcgattg cagatggcat tccgctgatg ggctacacca
    24601 catggggctg tattgattta gtttccgcct ctacgggtga aatgagcaaa cgctacggtt
    24661 ttgtctacgt agaccgtgac gacgcaggca acggcacgct gacgcgcaca cgtaagaaat
    24721 cgttctggtg gtataaaaaa gtgattgcca gtaatggtga agatttagag taagcaacag
    24781 tgccggatgc ggcgtgaacg ccttatccgg cctacatgtc gcacgaacca ggaaggcccg
    24841 ataagcgcag cgcatcgggc aatctggggt ttggcatcag tcaaaatcaa agaaaccatt
    24901 ccggcttaac gccaaatcgg gctgaaagtg ccttaatgtg ggatatcgtc agggatcgtt
    24961 gtccagacaa aatctgactg accagggatt tagagccgat ttcgttcttc aaatccgcat
    25021 aggtcagatt atgttgatca atcagcgtgc gaagtaacgc cacgccaaca ggcattgccg
    25081 caacggcttt gtcgaactct gcaaactttt cgttgttgtt ttcatactct gcaatgcggc
    25141 tcgccagaaa gtcgataagt ggattggtgt catcttcttc aatcaaatat tcgaccagtt
    25201 ccagtgcttc acgataatca tcttctgagt cactgcctcc cagaaagggg actgcattga
    25261 ccagttctct tgttgcttta acagcgcgtg cagcattagc agtcattctt tgttctctct
    25321 gtagtaacgt gtcagtttgt catactctgc gtgattcgtt atgtgcttca caaagaaccg
    25381 cttattaaca aagtttatgt aggcaataac cctaagattg ttcccgccga catccagaac
    25441 gtaccatttg ttgcgatatc taaaattgtc gaggttggga aacgccgttc gcatttcttc
    25501 tggtgtagaa aaatccgttt ccttaataac ccgatacaat gcctgatgag ccagagcgtc
    25561 gttaggatat ttacgcgcag actcttcaaa tggcgcttta gaaatgatgt gcatttttaa
    25621 gttttaactt ccttatagta agtaaacagc cacatctgcg atgttctatc cttatgtgtt
    25681 cggaaactat agctcatcaa atccaagttc acaatatgta aacacttgtg cgcgatataa
    25741 ttacccccaa aatcctcata gccactctgc aaaaagtcag ttctggttca cccctcaagc
    25801 acatccccct ttatcaagca acgctgcgtc tgcgccagat cttccgtttg cgggtcgcgc
    25861 ccggcgatgg gtttgaggac gagtttacgg ttgggggtat aagtaaaacg cgtgagcgcg
    25921 ggatagctta acttgccaaa ccagccgtga acacgctgcc atttggcgtc ttttacctga
    25981 tacagactgg ttatccagta accagaatta aactgcatat ccagcaattg cgtgtgacca
    26041 ttgccttgca aatccagcaa atcatctacg ccagttttgc ttgcggtgta gaatcccctg
    26101 ggttcaaaga cacagggacg gccttcgcgc gtgaacgtca taagaattaa atgtgcataa
    26161 ggagccagac cattaccggt acttggtaac cagataacca gatcctgaat gccgttacga
    26221 tcaagatcgg cgcgatagag ttgccctttg cccgctatgt ttgccagcat acccacaggc
    26281 acagtccagg atttaccggc gcggtcttta gcggttaatt gccagtaatc ttcttcattt
    26341 ggcatcttct caagcgtcac gccagcaacg cccactttcc taaaatgcca ggtttgctcg
    26401 gcatcataag gcatggtcag gagaggtttc agcgcccgct tttgcgcgta ttcgctgtca
    26461 ctcattggca gatattcggt aaagctggtg gcctgtgcag gcagcactaa gctcaataaa
    26521 aaagcgcaca tccctgttct gatgttcaat tccctgaatt ccctgatgga gaattattct
    26581 tcttccaccg ctaactgcgc aaatccacca tttccttccc agccttccag tcgttgataa
    26641 acggtttcta ccgcatcttt aatcggctgg gtcatcgggt agtaaaagcc gacgatatcc
    26701 ggctgaatgc cgaggaaaat cacttcacca atgtcttctt ttaactggtc gataaggtaa
    26761 ttcaacggca tgttatgggt agtcatcata aacatctcgg cgatatcatc cgggtcgatg
    26821 atgcggattt cgccggggtt tagccccata tccgtggcgt cgacaatcag cagtcgtgtc
    26881 gggcgcagtt cacggatggc gacgatgtcg ttttccggtg cactaccgcc gtcaatcacc
    26941 acccagttac ctttcggcgc ggcggcgcac ttttccgcca gcagcgggcc agcgccatca
    27001 tcgcccatca tgctattgcc aacacagagt aaaacgtcag tcacgcagtc tcctcaccat
    27061 caaatagatg gcgttttcct gatgaatatc atgcagcata cttaacagca ttgtactcca
    27121 ttccttctgc tgcgaagttt gcgctgcccg ggctttatcg aatgcgttgg ccagcatcgg
    27181 cacatggtta atgtcgatga cgatctcgcc atatttcggt acgccttcca ttttgcggcg
    27241 ggcttcgctg cccgcctcca gagtggcaat ccatgccaga tattcatccc atgggcaggt
    27301 gagcgccgct tccaggcaat cgataacccc aaggtggtga ccaatcgcca ggctgtaata
    27361 gaccacctgc tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt tacggctcag
    27421 ttgactgaac accacctttt cactcatcgg atacgcgcct cttcaacaac atgattcaga
    27481 tggctgacaa tctcgttcag acgcggatcg ttttccgctt ccagccagcg tgcaacctgt
    27541 tcttcgccct gccctaactg tgtaaggtaa tcatcggcaa tctgacgacc gtaacgataa
    27601 cccgccaggc gacgtgcttc gcgatccact ttcacgcgca gcggctgcac catatcgcca
    27661 tgcagaatct cagccggttg ttcatcctgc tcacccgggc cacgggcgtg aattttctgc
    27721 tccagcaggc cgagtgccat tgcaaagccg tacagcgtgg cggcaggcgt tggcgggcaa
    27781 cccggaatgt agacatctac cgggacgatt ttatccgtac cgccccacac gcagtagaga
    27841 tcgtggaaaa taccaccgct gttaccgcag gcaccgtagg agatacaaat tttcgggtcc
    27901 ggcgcggact gccacgcacg cagcgcaggg gatcgcattg cacgggtgac cgcgccggta
    27961 aacagtaaaa tatccgcatg acgcggtgaa ggaacgactt taatgccgaa gcgttctgca
    28021 tcaaacagcg gcgataaagt ggcgaaaatt tcgatttcgc agccgttgca gccgccgcag
    28081 tccacgcggt aaacataggc agaacgtttg atttttttca gtaacgacgc cttcatgctg
    28141 gcgatggatt catccaccgt catcgggacc ggaatgccgt tggcgtcacg ggggcctaat
    28201 aaattgctca tcagatggcc tctttcatat ggcgagtcag ttcaatacgg tcggacggca
    28261 ccaggcattt ctggcgctta cattccgggc aagtctcaaa gctttcgcgg tggttttccg
    28321 cgcggctgtc accattgtgc ttaagcagcg caatggcgta gtcgatctct ttctggacgg
    28381 cgaaaggacg attgcagacg cgacagctgc acagcacaaa acgggactgt tgcaggaaat
    28441 cttctttctt ccacaccgcc agttcgtact cttgcgacag tttgatcgct gccgtcgggc
    28501 agacttcttc gcagcgtcca cagaagatgc agcgcccgag attaaactcc cacgccagct
    28561 caccggtggc gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca ttgacgcagg
    28621 ccgcacagcc gatgcactgc tgcgggttct gctccggctt accacggaag tttttatcaa
    28681 ccgcaatcgg ctccagcgga taagacgagg tcgccgtgcc ggttttgatg acttttttga
    28741 taaaggtaaa catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac
    28801 gctcgagttc tttgtacggc accactttgc tctttttctt acgcacatcg accacggtca
    28861 tgcggtcggt acaggagtag caagggtcca ggctaccgat aatcagcggc gcatcggaaa
    28921 cggtgttacc gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc
    28981 agcgccagcg gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc
    29041 gcggcgcttc ggcaaagccc agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca
    29101 tcagcggacc gcccggcagg ttatccagac cgtagtcgat catgttcagc gcggtataaa
    29161 cttcgttgat acgcactttc agacgggaaa taacgtcgca gccctgctcg ctgtggactt
    29221 ccattggcag caggccataa ccgacaaacg ggtgatcggc gcgagtatcg cgggcgtgac
    29281 cgctggcgcg aaccatcggg ccaacgttac tgaaatcgcg ggcgatttcc gggtccagac
    29341 gaccaatgcc gacggtgcgc tgttccatgt tcggcgtact cagcagcaca tccaccagct
    29401 cctgcacttc acgacgcatc tgttgcgcca gctggcgggt ctggatcatg tcatctttca
    29461 acagatcgcg acgaatcccg ccgatcaggt tcaggccgta ggttttacgc gcaccggtga
    29521 ggatctctgc cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt
    29581 caaagccggt aaagtggcag gcgagaccga ggttgagcag atgcgagtgc aggcgttcca
    29641 cctccagcag aatggcgcgg atcatctgcg cacgttctgg cacctgaata cccatcgcgt
    29701 tttctaccga cgtggtgtag gcggtgctgt gggcaaagcc gcagatcccg cacacacggt
    29761 cagagaggaa ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt tccatgccgc
    29821 gatgaacgta gaacagacgg tagtcggcgt cgataatgtt ttcgccatcg acgaacagac
    29881 ggaagtggcc cggttcgtca gaagtgacgt gcagcggacc aatcggcacg acgttgtttt
    29941 tcttgtcgcc cagttcgttg atgaactcgt aggtttcagc atcggtagtc ggtgccggac
    30001 gctgacgata atccatgctg tctttacgca gcggataaag ttcatccggc cagtcatccg
    30061 gcagcaccag acgacgttca tccggcagac caaccggaat caaaccgtac atatcgcgca
    30121 cttcgcgctc gccccacacc gccgccggaa cgcgcggcgt cacggacgga tactccggtt
    30181 tgttggcgtc aacttcgacg cgaaccgtta cccaacactt ggtgcccttc tccatcgaca
    30241 gcacgtaata aacggcgtaa tgaccattca gtttgcgttc gtcgttacca aacagcaccg
    30301 acagccagcc accctgcttg tagtagagaa actccaccac ttccggcagg tagttcacct
    30361 tcacggtcac ggtcagctga tctttggtct gccaggcgtg gtccagcacg acgcccggaa
    30421 atgcctcatt cagcgcggcg agataatgtt gacctaattt ttcttcagac atgctcaaac
    30481 tctctttaat cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc caaacccagc
    30541 ccaggtaatg cgcggagtaa tatcaagacg cagacgggcc atgctgtttt cgaacagcgc
    30601 gataaccagg acgccgacga ccagtttgac gatggcaatc accagcgcca gcagcagccc
    30661 accggcggtg aaggtttcca tctgtcccca cgggataaat acgccgacga acatctgcag
    30721 caccaccagc tgtttcaggc tgatacccca tttcatgacg ccaaagccgc tgccgctgta
    30781 ttcagagagc gggccttcct gcagctcctg ctcggcttcc gccaggtcga acggcagttt
    30841 gcccatttcg ataaaggtgg cgaacgcaca ggcacaaagc gccagtacca gcgggatgct
    30901 ctggctcagc ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg tggaaccggc
    30961 aacctgtgcg gcaacccaca gaccaagcag cagcatcggt tcgaccagca cgccaagcat
    31021 cgcttcacgg ctcgcgccga tagcggtaaa cgggctaccg gtatccagac cagaaatggc
    31081 aaagaagaaa cgcgcgatgg caaagagata cagtaaggtg attaaatcac ccagttgcgg
    31141 cagcggagaa ccgacggtca ccaccggcag cgcagtagcg atagtcagca tgacgcccac
    31201 catcacatac ggcgtcaggc ggaacaccca gccggaggca tccgggccaa cgctctggcg
    31261 gcccagcagt ttgataatgt cacgatactc ctgcaacaca cccggcccac gacggttatg
    31321 taagcgggct cgcgccacgc gggtaatacc ggagagcagc ggcgcaacgg caaatagcac
    31381 cagcgcctga attaacggat ataaaacact cattctcagg ctcctcgtga aacaataatc
    31441 accaccagta ccgccagttc aaccagcgcc atccggcgga acagcaacgc actcccctcg
    31501 cactgccagc ccggcaccag agacaccgga ttcagccatt tgcgtagttt cagcaccggc
    31561 gcaaacgcct gtttcaccgg catggcaaaa ccgtgagcgg taatcaccat tgatttttcg
    31621 tggtcgtagc cgcacaccca ggccgcgccg cgggaacgcg atggcaaacg atcgcctttg
    31681 caaatcgcca taatgatgaa tggcagcagc ggacaggcaa tcagcagcaa tgtgatcatt
    31741 ggttgagaaa cggtggtgtt agcaggctcc agcggcagag gtacagcagc agacagcatc
    31801 ggcagcagcc acggcgcagc aacaccgcca attacgcagc aaatcgccag tgccactacg
    31861 cttaccctca tcaggagcgg cgcacaggtg gcgttttcgg cttctttggt acgcggcgcg
    31921 ccgaggaacg tgacgccata gactttcgcc atacacatca ccgccagcgc accggtaatt
    31981 gccagaccca cagcgagcag cggccccagc agacgggcaa caaacgcgcc gctattgctc
    32041 agtttgaaga aggattgata gataacccat tccccggcaa acccgttcag cggcggcagc
    32101 gcagccattg ccattaaccc gactaacatg gcgatggaga taaccggcat tttcttgcca
    32161 ataccaccga gtttttcgat atcgcgatga ccggtacgga accagacgct ccccgccccg
    32221 aggaacagta cgcttttgaa caggctatgg ttaagcagat ggtacagacc accgaccagg
    32281 ccaagagcaa tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc gcccagcccc
    32341 agcaggataa tgccgatatt ttccagggtg tggtaagcca gcaggcgctg gatgttgtgc
    32401 tccatcagcg catacagacc accgacaaac gcggtgatca tgccgagcac cagcagcgcg
    32461 atcccccacc acagcggtgc attaccgccc agcagtgaca gggttaaaat gcccagcagg
    32521 ccaattttca tgactaccgt agaaaacaac gcggcagctg gcgcagaggc gttagcatgt
    32581 gcctgcggca cccagccgtg cagcggaata atcccggcga gcaggccaaa gccaatcaca
    32641 ccgagcagcc agatatcgga accgagcggc agctgttgca tacgcatatc cagcaggcgc
    32701 agatccagcg tgccataacg ctgccacagc agccagcaag caatcgccag cagcagagtg
    32761 ccaagacgcc ccagcgcaaa ccacagtttg ccctctttgc tgttgctagt gaggaacacc
    32821 gcgcacaggg ccatgatttc ggccattacc acgaacatgc cgaggttgct ggcaatgacg
    32881 gcgcagacgg cggcagccat caacatattg atctgcaagc cgttgcactt cacctgcgcg
    32941 tggcgatgcc agtcaatgtt atagaggctg acaaacagac cgcaaaggcc gagggtaatc
    33001 agccaaatcg cgttaagcgg ggagatttgc acatcgtagc ttaccagcga cagcgcaccg
    33061 ctcacgccaa ccgcgccagt cagcacagtg aagcccgcgg ctgccgtata caggctacca
    33121 accgcgccgc caattccggc tatccagcca cttagcgtct tttgaaaaga aaagagaaat
    33181 gccagaacag cggcggcgac aaaccacgcc acgccgctat tgatcaggga aattgcgctc
    33241 atttagcctc tccactttga gcctgctgaa acaaggtgag atcgccaaag tccgtgttaa
    33301 aggtcagctc acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc accagatgca
    33361 gggctttagt cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag ctacaaaggt
    33421 cacatttgac ggcgatcgcg cgaatacctg gcacccagtc aagcaatgtg ctgacacgcg
    33481 ccggagcagg cggtgccggt ggcgctttcg gggtattggc gtttgccgga atatccagcg
    33541 gacggctgcc ggaaaattca attgcgccaa acgggcaggc gatgccgcac agcttgcagc
    33601 ttacgcacag gctttcattc aactgcacgg ccccatcaac gcgggtgatg gcgttaaccg
    33661 ggcagaccac cgcgcagggt gcatcttcac agtggtgaca gagctgcggc gcagattctt
    33721 tttcattcag catcactctc aggcgcggca ttgattgcag gccgtgctgg cgatgcgtct
    33781 ctgaacaggc ggcctcacaa gtgtggcagc cgatacagag cgtggagtca gcaattacaa
    33841 aacgattcac caggcattcc tcaggtgatt gtcatttttg acgaaaacat gccgatgaaa
    33901 tgtcattttc gacactcatc gacacgccca tccccaaaca ggcgtaacgc ctgcaaaacg
    33961 ggcaaagcct cagctcatgc tgccgggctt tgtcccttta ccagttggct taaattcacc
    34021 ggcacattgt tttcaatagc ggtgtataag ctgtcgtaaa cgccagcgat tttttcgaga
    34081 tagtgttcca gaacctgttc gcgatcgcgg atgttgacct tgccatcaat catgccgtca
    34141 atgctcagtt gcgcctgcgc aatccgctgt ttgtcttcgc catcttttgt ttcgacgtaa
    34201 tactcgatgg tgtggctgtt aaagccatcc gccagggtga cgtaaatgcg aaaatgttca
    34261 aaaaggacga aacagagttc tttgtccggc gcgcagctca tggcgtgatg caggcgcgct
    34321 ttcgataacg tgatcccctg aaccagcgaa ttgcagtaga tgtgccactg gtcctgtagg
    34381 cgacgatgcc gctgtgcgat gtaatcggct ttctcgctta tttcccaaat agtcattgtc
    34441 aggttacccg tttaacagag atgccagctt taagcatttt ctgtgccaac ttttaattta
    34501 ttgttattaa agagattttt aagctaaaga tgaatttcgt cgccgtgtcg acgtgtcatt
    34561 tcgacatcat cgacattatt caccgcaggg ataatcaaca ctggcacaat tattgcttgt
    34621 agctggcaat agttaatggg aggcgatatg cacgaaataa ccctctgcca acgggcactg
    34681 gaattgatcg aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg ggtctggctc
    34741 aaaattggcg cattttcttg tgtcgaaacc agctctcttg ccttttgttt tgatctggtt
    34801 tgccgcggca gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga ggccgaatgc
    34861 tggtgtgaaa catgccaaca gtatgtgacg ctactgaccc agcgcgtccg ccgctgtcca
    34921 cagtgtcatg gtgacatgct gcagattgtg gcagacgacg gtttacagat tcggcggata
    34981 gaaatagacc aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg caacctgtat
    35041 atcgagggtg atgaacataa ccctcattcc gcgtttcgta gcgcgccatt tgccccggcg
    35101 gcacgcccga agatgaaaat caccggcatt aaagcgcctg aatttacccc cagccagact
    35161 gaagaaggcg acctgcatta cggtcatggc gaagcgggca ctcacgcacc gggcatgagc
    35221 cagcgtcgga tgctggaagt cgaaattgac gtgctggaca aaaataaccg tctggctgaa
    35281 cgcaaccgcg cgcgctttgc tgcccgcaag caactggtgc tcaacctggt ttccagccca
    35341 ggttccggta aaaccaccct gctgacggaa accttaatgc gcctgaaaga cagcgttccg
    35401 tgcgcggtta ttgaaggtga ccagcaaacc gtgaacgatg ccgcacgcat tcgcgctacc
    35461 ggcacaccag cgattcaggt gaacaccggt aaaggctgcc atcttgacgc acagatgatt
    35521 gccgacgccg caccgcgtct gccactggac gataacggta ttctgtttat cgaaaacgtt
    35581 ggcaacctcg tatgcccggc cagcttcgat ctcggtgaaa aacacaaagt ggcggtgctt
    35641 tccgttaccg aaggtgaaga caaaccgctg aaatatccgc atatgtttgc cgccgcctcg
    35701 ctgatgctgc tcaacaaagt tgacctgttg ccgtatctca actttgacgt tgagaagtgc
    35761 ctcgcctgcg cccgcgaagt caatccagaa attgaaatca tccttatttc cgccaccagc
    35821 ggtgaaggga tggaccagtg gctgaactgg ctggagacac agcgatgtgc ataggcgttc
    35881 ccggccagat ccgcaccatt gacggcaacc aggcaaaagt cgacgtctgc ggcattcagc
    35941 gcgatgtcga tttaacgtta gtcggcagct gcgatgaaaa cggtcagccg cgcgtgggcc
    36001 agtgggtact ggtacacgtt ggctttgcca tgagcgtaat taatgaagcc gaagcacgcg
    36061 acactctcga cgccttacaa aacatgtttg acgttgagcc ggatgtcggc gcgctgttgt
    36121 atggcgagga aaaataatgc gttttgttga tgaatatcgc gcgccggaac aggtgatgca
    36181 gttaattgag catctgcgcg aacgtgcttc acatctctct tacaccgccg aacgccctct
    36241 gcggattatg gaagtgtgcg gtggtcatac ccacgctatt tttaaattcg gcctcgacca
    36301 gttactgcct gaaaacgttg agtttatcca cggtccgggt tgcccggtgt gcgtactgcc
    36361 gatgggtaga atcgacacct gcgtggagat tgccagccat ccggaagtca tcttctgtac
    36421 ctttggcgac gccatgcgcg taccagggaa acagggatcg ctgttgcagg caaaagcacg
    36481 cggtgccgat gtgcgcatcg tttactcgcc gatggatgcg ttgaaactgg cgcaggagaa
    36541 tccaacccgc aaagtggtgt tcttcggctt aggttttgaa accaccatgc cgaccaccgc
    36601 catcactctg caacaggcga aagcgcgtga tgtgcagaat ttttacttct tctgccagca
    36661 cattacgctt atcccgacgc tgcgcagttt gctggaacag ccggataacg gtatcgacgc
    36721 gttcctcgcg ccgggccacg ttagtatggt tatcggcact gatgcctata attttatcgc
    36781 cagcgatttt catcgtccgc tggtggtcgc tggtttcgaa ccccttgatc tactgcaagg
    36841 cgtggtcatg ctggtggagc agaaaatagc ggcccacagc aaggtagaga atcagtatcg
    36901 tcgggtggta ccggatgccg gtaacctgct ggcgcaacag gcgattgccg atgtgttctg
    36961 tgtcaacggc gacagcgaat ggcgcggctt aggcgtgatt gaatcttctg gcgtgcacct
    37021 gacgccggat tatcaacgat tcgatgccga agcacatttc cgcccggcac cgcagcaggt
    37081 ctgcgatgac ccgcgcgcgc gttgtggtga ggtattaacg ggcaaatgta agccgcatca
    37141 atgcccgctg tttggtaaca cctgtaatcc tcaaaccgca tttggtgcac tgatggtctc
    37201 ctccgaagga gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg aagcgtgaat
    37261 aatatccaac tcgcccacgg cagcggcggc caggcgatgc agcaattaat caacagcctg
    37321 tttatggaag cctttgccaa cccgtggctg gcagagcagg aagatcaggc acgtcttgat
    37381 ctggcgcagc tggtagcaga aggcgatcgt ctggcgttct ccaccgacag ttacgtcatt
    37441 gacccgctgt tctttcctgg cggtaatatc ggcaagctgg cgatttgcgg cactgcgaat
    37501 gacgttgcgg tcagtggcgc tattccgcgc tatctctcct gtggctttat cctcgaagaa
    37561 ggattgccga tggagacact gaaagccgta gtgaccagca tggcagaaac cgcccgcacg
    37621 gcaggcattg ccatcgttac tggcgatact aaagtggtgc agcgcggcgc ggcagataaa
    37681 ctgtttatca acaccgctgg catgggcgca attccggcga atattcactg gggcgcacag
    37741 acgctaaccg caggcgatgt attactggtt agcggcacgc tcggcgacca cggggcgact
    37801 atccttaacc tgcgtgagca gctggggctg gatggcgaac tggtcagcga ctgcgcggta
    37861 ctgacgccgc ttattcagac gctgcgtgac attcccggcg tgaaagcgct gcgtgatgcc
    37921 acccgtggtg gtgtgaacgc ggtagttcat gagttcgcgg cagcctgcgg ttgtggtatt
    37981 gaaatttcag aagcggcact gccggttaaa cctgccgtgc gcggcgtttg cgaactgctg
    38041 ggactggacg cccttaactt tgccaacgaa ggcaaactgg tgatcgccgt tgaacgcaac
    38101 gcggcagagc aagtgctggc agcgttacat tcccatccac tgggaaaaga cgcggcgctg
    38161 attggtgaag tggtggaacg taaaggtgtt cgtcttgccg gtctgtatgg cgtgaaacga
    38221 accctcgatt taccacacgc cgaaccgctt ccgcgtatat gctaataaaa ttctaaatct
    38281 cctatagtta gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga agaacaaaat
    38341 gtcatataca ccgatgagtg atctcggaca gcaagggttg ttcgacatca ctcggacact
    38401 attgcagcag cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg taaagcgttc
    38461 tgcgctcgcc gacaacgcgg ctattgtgtt gtggcaagcg cagactcaac gtgcgtctta
    38521 ttacgcatcg cgtgaaaaag acacccccat taaatatgaa gacgaaactg ttctggcaca
    38581 cggtccggta cgccgcattt tgtcgcgccc tgatacgctg cattgcagtt acgaagaatt
    38641 ttgtgaaacc tggccgcagc tggtcgcagg tgggctatac cccaaatttg gtcactattg
    38701 cctgatgcca ctggcggcgg aagggcatat ttttggtggc tgtgaattta ttcgttatga
    38761 cgatcgtccc tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc agatcgtttc
    38821 tgtcgtcacc gaacaaatcc agagccgcgt cgtcaacaat gtcgactatg agttgttatg
    38881 ccgggagcgc gataacttcc gcatcctggt tgccattacc aacgcggtgc tttcccgcct
    38941 ggatatggac gaactggtca gcgaagtcgc caaagaaatc cattactatt tcgacattga
    39001 cgatatcagc attgtcttac gcagccaccg taaaaacaaa ctcaacatct actccactca
    39061 ctatcttgat aaacaacatc ccgcccacga acagagcgaa gtcgatgaag ccggaaccct
    39121 caccgaacgc gtgttcaaaa gtaaagagat gctgctgatc aatctccacg agcgggacga
    39181 tttagccccc tatgaacgca tgttgttcga cacctggggc aaccagattc aaaccttgtg
    39241 cctgttaccg ctgatgtctg gcgacaccat gctgggcgtg ctgaaactgg cgcaatgcga
    39301 agagaaagtg tttaccacta ccaatctgaa tttgctgcgc cagattgccg aacgtgtggc
    39361 aatcgctgtc gataacgccc tcgcctatca ggaaatccat cgtctgaaag aacggctggt
    39421 tgatgaaaac ctcgccctga ccgagcagct caacaatgtt gatagtgaat ttggcgagat
    39481 tattggccgc agcgaagcca tgtacagcgt gcttaaacaa gttgaaatgg tggcgcaaag
    39541 tgacagtacc gtgctgatcc tcggtgaaac tggcacgggt aaagagctga ttgcccgcgc
    39601 gatccataat ctcagtgggc gtaataatcg ccgcatggtc aaaatgaact gcgcggcgat
    39661 gcctgccgga ttgctggaaa gcgatctgtt tggtcatgag cgtggggctt ttaccggtgc
    39721 cagcgcccag cgcattggtc gttttgaact ggcggataaa agctctctgt tcctcgacga
    39781 agtgggcgat atgccactgg agttacagcc gaagttgctg cgtgtattgc aggaacagga
    39841 gtttgaacgt ctcggcagca acaaaatcat tcagacggac gtacgtttaa ttgccgcgac
    39901 taaccgcgat ctgaaaaaaa tggtcgccga ccgagagttc cgtagcgatc tctattaccg
    39961 cctgaacgta ttcccgattc acctgccgcc actacgcgag cgtccggaag atattccgct
    40021 gctggcgaaa gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata tcgacagcat
    40081 tcctgccgag acgctgcgca ccttgagcaa catggagtgg ccgggtaacg tacgcgaact
    40141 ggaaaacgtc attgagcgcg cggtattgct aacacgcggt aacgtgctac agctgtcatt
    40201 gccagatatt gttttaccgg aacctgaaac gccgcctgcc gcaacggttg tcgcccagga
    40261 gggcgaagat gaatatcagt tgattgtgcg cgtgctgaaa gaaaccaacg gcgtggttgc
    40321 cgggcctaaa ggcgctgcgc aacgtctggg gctgaaacgc acgaccctgc tgtcacggat
    40381 gaagcggctg ggaattgata aatcggcatt gatttaactg caaattgccg gacagatctg
    40441 cctgtccggc atactattca cgaggttttt tcggacgata tttttccggc agttctggca
    40501 ccggacgctt gtcatcgatg agatgacgca cggttaagat cggatgacgc cacagcattc
    40561 tcggcccggc ccaacgcata atctgtttca tctcttcacg ctttgcaggc tggtaacagt
    40621 gcaccggaca ctgcttacag gctggtttct cttcgccgaa cacacattta tccagccgct
    40681 tttgcgcgta aacaaacaac gcctcgtaat gctccggctc cgctgacgcc tgcgggcatt
    40741 tcgcttgata aagatcgatc atttttttaa tcgtcagttt ttcacgagag atacgcttgc
    40801 cggacatgct gcctccatac tcttaaggtg tatttatatt acaacttaat ttgaaagggg
    40861 actatggctc tgaaggagga ggtttattca accgacgcaa ttttgcgtac ttgcttcata
    40921 agcatcacgc aaaagctgca aaacagcatc tttcccggaa ccagcatcaa gaactcgcag
    40981 ttcgcttctt cccctgaaat gattaactcc ggtatcatgt gcgccttatg tgattacaac
    41041 gaaaataaaa accatcacac cccatttaat atcagggaac cggacataac cccatgagtg
    41101 caatagaaaa tttcgacgcc catacgccca tgatgcagca gtatctcagg ctgaaagccc
    41161 agcatcccga gatcctgctg ttttaccgga tgggtgattt ttatgaactg ttttatgacg
    41221 acgcaaaacg cgcgtcgcaa ctgctggata tttcactgac caaacgcggt gcttcggcgg
    41281 gagagccgat cccgatggcg gggattccct accatgcggt ggaaaactat ctcgccaaac
    41341 tggtgaatca gggagagtcc gttgccatct gcgaacaaat tggcgatccg gcgaccagca
    41401 aaggtccggt tgagcgcaaa gttgtgcgta tcgttacgcc aggcaccatc agcgatgaag
    41461 ccctgttaca ggagcgtcag gacaacctgc tggcggctat ctggcaggac agcaaaggtt
    41521 tcggctacgc gacgctggat atcagctccg ggcgttttcg cctgagcgaa ccggccgacc
    41581 gcgaaacgat ggcggcagag ctgcaacgca ctaatccggc ggaactgctg tatgcagaag
    41641 atttcgccga gatgtcgctg attgaaggcc gtcgcggcct gcgccgtcgc ccgctgtggg
    41701 agtttgaaat cgacaccgcg cgccagcagt tgaatctgca atttggcacc cgcgatctgg
    41761 tcggttttgg cgtcgagaac gcgccgcgcg gactttgtgc tgccggttgt ctgttgcagt
    41821 atgcgaaaga tacccaacgc acgaccctgc cgcatattcg ttctatcacc atggaacgtg
    41881 agcaggacag catcattatg gatgccgcga cacgtcgtaa cctggaaatt actcagaacc
    41941 tggcgggcgg tgcggaaaat acgctggctt ctgtgctcga ctgcaccgtc acgccgatgg
    42001 gtagtcgtat gctgaaacgc tggctgcata tgccagtgcg cgatacccgc gtgttgcttg
    42061 agcgccagca aactattggc gcattgcagg atttcaccgc cgagttgcag ccggtactgc
    42121 gtcaggtcgg cgacctggaa cgtattctgg cacgtctggc tttacgaact gctcgcccac
    42181 gcgatctggc ccgtatgcgc cacgctttcc agcaactgcc ggagctgcgt gcgcagttag
    42241 aaactgtcga tagtgcaccg gtacaggcgc tacgtgagaa gatgggcgag tttgccgagc
    42301 tgcgcgatct gctggagcga gcaatcatcg acacaccgcc agtgctggta cgcgacggtg
    42361 gtgttatcgc atcgggctat aacgaagagc tggatgagtg gcgcgcgctg gctgacggcg
    42421 cgaccgatta tctggagcgt ctggaagtcc gcgagcgtga acgtaccggc ctggacacgc
    42481 tgaaagttgg ctttaatgcg gtgcacggct actacattca aatcagccgt gggcaaagcc
    42541 atctggcacc catcaactac atgcgtcgcc agacgctgaa aaacgccgag cgctacatca
    42601 ttccagagct aaaagagtac gaagataaag ttctcacctc aaaaggcaaa gcactggcac
    42661 tggaaaaaca gctttatgaa gagctgttcg acctgctgtt gccgcatctg gaagcgttgc
    42721 aacagagcgc gagcgcgctg gcggaactcg acgtgctggt taacctggcg gaacgggcct
    42781 ataccctgaa ctacacctgc ccgaccttca ttgataaacc gggcattcgc attaccgaag
    42841 gtcgccatcc ggtagttgaa caagtactga atgagccatt tatcgctaac ccgctgaatc
    42901 tgtcgccgca gcgccgtatg ttgattatta ccggcccgaa catgggcggt aaaagtacct
    42961 atatgcgcca gaccgcactg attgcgctga tggcctacat cggcagctat gtaccggcac
    43021 aaaaagtcga gattggacct atcgatcgca tctttacccg cgtaggcgcg gcagatgacc
    43081 tggcttccgg gcgctcaacc tttatggtgg agatgactga aaccgccaat attttacata
    43141 acgccaccga atacagtctg gtgttaatgg atgagatcgg gcgtggaacg tccacctacg
    43201 atggtctgtc gctggcgtgg gcgtgcgcag aaaatctggc gaataagatt aaggcattga
    43261 cgctgtttgc cacccactat ttcgagctga cccagctacc ggagaaaatg gaaggcgtcg
    43321 ccaacgtgca tctcgatgcg ctggagcacg gagacaccat tgcctttatg catagcgtgc
    43381 aggatggcgc ggcgagcaaa agctacggcc tggcggttgc agctctggca ggcgtgccaa
    43441 aagaggtgat taagcgcgca cggcaaaaac tgcgtgagct ggaaagcatt tcgccgaacg
    43501 ccgccgctac gcaagtggat ggtacgcaaa tgtctttgct gtcagtgcca gaagaaactt
    43561 cgcctgcggt cgaagcactg gaaaatctcg acccggattc actgactccg cgtcaggcgc
    43621 tggagtggat ttatcgcttg aagagtctgg tgtaataaca attcccgata gtcttttgct
    43681 atcgggaata ttaacgacaa ctgacgaatc aaataaaaat accctgtata ataggaaagc
    43741 ttattttaca gggtaaaacc atgccatcta cacgctatca aaaaatcaat acccatcact
    43801 atcgccatat atgggtcgtt ggtgatattc atggtgaata tcagttatta caatcccgct
    43861 tacatcaact ctcttttttc cccgaaaccg acttacttat ttctgtcggc gataatattg
    43921 atcgtgggcc ggagagtctt gacgtcctgc gcctgctaaa ccagccctgg tttatctccg
    43981 ttaaaggcaa ccacgaagca atggcgctgg atgcgttcgc gaccggcgat ggcaatatgt
    44041 ggcttgccag cggtggtgac tggtttttcg atttaaatga ttcagagcaa aaagaagcta
    44101 cagatctgtt gctgaaattc catcaccttc cacatattat tgaaatcact aacgacaaca
    44161 taaaatatgt catcgcacat gcagattatc cggggagtga atatctcttt ggtaaagaaa
    44221 tagcggagag cgaattactc tggcctgttg atcgtgtgca gaaatcgctt aatggcgagt
    44281 tacaacaaat aaacggcgct gattatttta tatttggaca tatgatgttt gataacattc
    44341 agacgttcgc taaccagatt tatattgata ccggcacgcc gaaaagcggg cggctgtcat
    44401 tttataaaat aaagtagtct catgcttctt ctgtgaagca tgagtaacca aatgttattg
    44461 caggccatta ttcatttttc gctaccagca aagagagatc ctgcttcgcc agcgcgcgac
    44521 tggcactctc cggcaaaccg tcgtctgtaa taatctgatc aaactcgctt aatggtaacg
    44581 ccagccatgt cgccacctga ccatatttcg tcgcatcaca gaccaaaact cgctggcggc
    44641 tggcactggc aatcgcccgt ttcaccgtga ctttatcttc cgctggcgta gaaatccccc
    44701 gcacactcca tgacgatgca gaaataaaag cctgatcaat catcaggctg cgcagcatgg
    44761 tcgcagcggc ttccccgaca caggaacggt tttcccgaca cactgcaccg ccagtgtgaa
    44821 taattgtgca attactgttg tcgagcaagt agttcgcaat aacgaaatcg tttgtgacca
    44881 cagtcagtga ctccatgtga atcagatgct gtgctatcgc taacgtggtc gttcccgcat
    44941 ccagatagat acaacttccc ggctgaacaa gacttgccgc cagcttgcca atagccgctt
    45001 tttgcgtcat tgccagcgca gtttttacct gatgagaagg ttcatgcgcc acgcgtcccg
    45061 gagactggac gcctccggac accagcacaa cggctccctg ctgctccagt ttttgtaaat
    45121 cccgacgaat ggtcatatgt gacacattca ttctgtccgt tagttcagca atactgacaa
    45181 tgcctttttc agctaccatc tcaaggatga tttggcgacg ctctacgggt atcaactttt
    45241 gctccttcct ttgtcctgct gacattctac gctatttgcc tgcgaaacgt gcgcggcgca
    45301 actaacgctt agttcacata aaataacaca caatgttaat ttatgtgaat cagctcacca
    45361 tatcgttatc ttccagcgct tatattcaca atatcaaaca aaatatcact taaattaaca
    45421 aggagagcag atgaaaacgg gatctgagtt tcatgtcggt atcgttggct tagggtcaat
    45481 gggaatggga gcagcactgt catgtgtccg cgcaggtctt tctacctggg gcgcagacct
    45541 gaacagcaat gcctgcgcta cgttgaaaga ggcaggtgct tgcggggttt ctgataacgc
    45601 cgcgacgttt gccgaaaaac tggacgctct gctggtgctg gtggtcaatg cggcccaggt
    45661 taaacaggtg ctgtttggtg aaacaggcgt tgcacaacat ctgaaacccg gtacggcagt
    45721 aatggtttct tccactatcg ctagtgctga tgcgcaagaa attgctaccg ctctggctgg
    45781 attcgatctg gaaatgctgg atgcgccagt ttctggtggt gcagtaaaag ccgctaacgg
    45841 tgaaatgact gtcatggcct ccggtagcga tattgccttt gaacgactgg cacccgtgct
    45901 ggaagccgtt gccggaaaag tttatcgcat aggtgcagaa ccgggactag gttcgaccgt
    45961 aaaaattatt caccagttgt tagcgggcgt acatattgct gccggagccg aagcgatggc
    46021 acttgcagcc cgtgcgggga tcccgctgga tgtgatgtat gacgtcgtga ccaatgccgc
    46081 cggaaattcc tggatgttcg aaaaccggat gcgtcatgtg gtggatggcg attacacccc
    46141 gcattcagcc gtcgatattt ttgttaagga tcttggtctg gttgccgata cagccaaagc
    46201 cctgcacttc ccgctgccat tggcctcaac agcattgaat atgttcacca gcgccagtaa
    46261 cgcaggttac gggaaagaag acgatagcgc agttatcaag attttctctg gcatcactct
    46321 accgggagcg aaatcatgat caagattggc gttatcgccg atgattttac cggcgcgacg
    46381 gatatcgcca gttttctggt ggaaaacggt ctaccaacgg tacaaattaa cggtgttcca
    46441 acaggtaaaa tgccggaagc aatcgacgca ctggtgatca gcctgaaaac gcgctcctgt
    46501 ccggtggttg aagccacaca gcaatcgctg gcggctctga gctggttgca acagcaaggt
    46561 tgcaaacaga tctatttcaa atactgctct actttcgaca gtacggcgaa aggtaatatc
    46621 ggcccggtta ccgatgcatt aatggatgct ctcgacacgc cgtttacggt cttctctccg
    46681 gccctgccgg tcaacggacg tacggtttat caggggtatt tgttcgtaat gaatcaactg
    46741 ctggccgaat ccgggatgcg ccatcatccg gtaaatccca tgaccgacag ctatcttccc
    46801 cgtctggttg aagcgcaatc cacagggcgc tgcggcgtcg tttcggcaca tgttttcgaa
    46861 caaggtgtgg atgccgttcg tcaagagctg gctcgcttac agcaagaggg ctaccgctac
    46921 gcggtgcttg atgcgctgac cgaacaccat ctggaaattc agggagaagc cttgcgcgat
    46981 gccccactgg taacgggcgg ttctggtctg gcgattggcc tggcccggca gtgggcgcaa
    47041 gaaaacggta accaggctcg cgaagcaggg catccgctcg ctgggcgcgg cgtagtgctc
    47101 tccggttcat gctctcaaat gaccaaccgc caggtggcac attaccgtca aattgcacca
    47161 gcccgtgaag ttgatgtggc acgctgcctc tcaaccgaaa ctctggccgc ttatgcacac
    47221 gaactggcag agtgggttct gggccaggaa agtgtacttg ctccactggt ttttgccacc
    47281 gccagcactg acgcattggc agcaattcaa cagcaatacg gtgcacaaaa agccagtcag
    47341 gcagtagaaa ctctattttc tcaactagcg gcgcggttag cagcggaagg cgtgacacgc
    47401 tttattgtcg caggcggtga gacctccggc gtagtcacac agagcctggg cataaaaggg
    47461 tttcatattg gcccaaccat ttcccccggc gtgccgtggg taaacgcact ggataagcct
    47521 gtctcactcg cccttaaatc tggcaacttc ggtgatgaag cctttttttc acgagcccaa
    47581 agagagtttt tatcatgagc gatttcgcaa aagtagagca gtctttgcga gaggagatga
    47641 cgcggattgc cagttcattc tttcagcgcg gctatgcaac cggttcggct ggcaatctgt
    47701 cgctgctttt acctgacggg aatttactgg cgacaccgac aggttcatgc ctgggcaatc
    47761 tcgatccgca gcggctttcc aaagtcgccg cggatggcga atggttaagt ggtgacaaac
    47821 cctcgaaaga ggtgctcttt catctggcgc tgtatcgcaa caatccgcgc tgtaaagcgg
    47881 tggtgcattt gcacagcaca tggtcgacgg cgctttcctg cctgcaaggg ctggacagca
    47941 gcaacgttat tcgtccgttc acaccatacg tggtgatgcg gatgggaaat gtcccgctgg
    48001 tgccttatta ccgaccgggc gataaacgca tcgcacagga tctggcggaa ctggcagcag
    48061 acaatcaggc ttttttactg gcaaatcatg gcccagtggt ttgcggtgaa agcctgcaag
    48121 aagccgccaa caatatggaa gagctggagg aaacggcaaa gctgattttt attctcggtg
    48181 accgcccgat ccgttatctg accgcaggtg aaattgcgga attaaggagt taatgcaatg
    48241 cctcgttttg cagctaattt atccatgatg ttcaccgaag tgccttttat tgaacgcttc
    48301 gccgcagcgc gaaaagccgg gttcgatgct gtggaatttc tgtttcccta tgactactcc
    48361 accctgcaaa tccaaaagca actggagcaa aaccatctga cactggcgct gttcaatacc
    48421 gctcccggag atattaatgc gggggaatgg ggattatccg cccttcccgg acgtgagcac
    48481 gaagcacacg cagacatcga cctggcactg gaatatgcat tggcgctcaa ttgtgaacaa
    48541 gtccatgtga tggcaggcgt cgtgcccgct ggcgaagacg ctgaacggta ccgggcagta
    48601 tttatcgata atctccgcta cgctgccgac cgctttgcgc tacacggcaa gcgaatatta
    48661 gttgaagcac tcagccccga cgttaagcct cattatctct tctccagcca gtatcaggca
    48721 ctggctattg ttgaagaggt tgcgcgagat aacgtgttta ttcaactaga tacttttcat
    48781 gcgcaaaaag tagatggcaa cctgacgcat ttaatccgcg actacgctgg gaaatatgcg
    48841 cacgtacaaa ttgccggact acctgatcga catgaaccgg acgatggaga aatcaactat
    48901 ccgtggctgt tccgcctgtt cgatgaggtg ggatatcagg gctggatcgg ttgtgaatat
    48961 aaacctcgtg gcctcaccga agaagggctt ggctggtttg acgcctggcg ctaattcgtt
    49021 aaatccctaa ttacaacgta cccatacatc cccccatcta cggatggggt aggttaacta
    49081 taactttcag acagggtttc ccatgtccac aattacattg ttatgcattg cgttagctgg
    49141 cgtaatcatg ctgttgctgc tggtcatcaa ggcaaaggta caaccattcg ttgctctgct
    49201 cctcgtcagc ctgttagtcg cacttgcggc gggtataccg gcgggcgaag tgggtaaagt
    49261 gatgatcgcc gggatgggcg gtgtgcttgg ttccgtcact attattattg gtctgggcgc
    49321 tatgctgggg cgtatgatcg aacactctgg tggtgcagag tcactggcta attatttcag
    49381 tcgcaagtta ggtgacaaac gaactatcgc tgcgctgact ctggcagcgt tcttcctcgg
    49441 tattcccgtc ttctttgatg tcggctttat tattcttgcg ccaatcattt acggttttgc
    49501 caaggttgcc aaaatatcgc cactcaaatt tggcctgcct gtcgctggaa tcatgctaac
    49561 tgttcatgtg gcggtaccgc cgcatccagg ccctgtcgcc gcagcggggt tactccacgc
    49621 agacatcggc tggctaacca tcatcggtat tgcgatttct attcccgtag ggattgttgg
    49681 ctactttgca gcgaaaataa tcaatacgcg ccaatatgcg atgtcggtgg aagtactgga
    49741 acagatgcaa ctggctccgg ccagtgagga aggcgcaaca aaattaagcg ataaaataaa
    49801 tccaccgggc gtcgcgctgg tcacctcgct aattgttatt cctatcgcga ttatcatggc
    49861 gggtacggtt tccgcaacac tgatgccgcc ttcgcatccc ctgcttggta cgctacagct
    49921 gatcggctca ccaatggtag ctctaatgat tgcgctggtg ctggcattct ggttattggc
    49981 tttacgtcgc ggctggagct tacaacacac cagcgacatt atgggctcag cccttcccac
    50041 tgcggcggta gtgattttgg ttactggtgc tggaggggta tttggcaaag tgctggtgga
    50101 atcgggcgtc ggcaaagccc ttgccaatat gctacaaatg attgacctgc ctctgttacc
    50161 agccgcattt attatttcac tggctctgcg tgcatcgcag gggtcagcaa ccgtagcaat
    50221 cctgacaacc ggcgggttac tctcagaagc ggtgatggga ttgaatccga ttcagtgcgt
    50281 attggtgacg ctggcagcct gctttggtgg gcttggcgcg tcacatatta atgactcagg
    50341 gttctggatt gtgaccaaat atctggggtt gtcggtagca gacggtctga aaacctggac
    50401 agtgttaacg accattctcg gttttaccgg attcttaatt acctggtgcg tatgggcggt
    50461 aatttgacct taataaaaag gtccgatggg catcggacct tttatttaac gcttaccttc
    50521 caccagcagc ggcgggatgc tcggcaccat tggcgcgtca tcaatatctt tctgcgtcat
    50581 gcggaacgct tcgggataat gttcgcggct ggtacggcgc agcgattcgg tatcgcgcca
    50641 ggtataaagg caatgctggc actgatatac cgtccagaca tctttcaccg gcgatttcgc
    50701 catcacttca atctgttcat cggcacaacg tggacaaatc atcttcttct ccttatttac
    50761 gtgcagccag catagcggtc agtttttcag cccaggcttt ggtttccggt aaatccacca
    50821 ccggttggct gtagtgacca cggttgtccg gggcgacagg cgtagtggcg tcgataatca
    50881 gcttgtcggt gatccccgca gggcttgagc ctggatcgag ttccagcacg gacatattcg
    50941 gcaactgcac caaatcccct gccgggttca ctttcgagga gagcgcccac atcacctgcg
    51001 gcaggttgaa cgggtcaacg tcttcatcga ccataatcac catcttcacg tagcccagac
    51061 catgcggcgt ggtcattgcg cgcaggccca ccgcgcgggc aaagccgccg tagcgttttt
    51121 tggtggagat aatcgccagc aggccatggg tgtacatggc gtttaccgcc tgcacttcag
    51181 ggaactcggc tttcagctgc tgatacagcg gcacgcaggt ggctggcccc atcaggtagt
    51241 cgatttcggt ccacggcata ccgaggtaca gcgattcgaa aatcggcctg gtgcggtaag
    51301 agactttatc gatgcgcacc acggtcatgt tacgcccgcc ggagtagtgc ccggtaaact
    51361 caccgaacgg cccttcgatt tcgcgtttac ggctttcgat gaccccttcg aggatcactt
    51421 ctgaacccca cggcacatca aaaccggtca acggggcggt ggcgatcggg tacgggcttt
    51481 cacgcagcgc gcctgccatt tcgtactcgg actgatcata tttcagcggc gtggccccca
    51541 tcagcgtgat gatcggatcg ttaccgagcg tgatcgcaat cggcagatct tcaccgcgct
    51601 cttctgcttt atgcagatgc agggcgatat cgtgcatcgg caccggttgc aggccgagct
    51661 tacgcttgcc cttcacttcc atgcggtaga tgccgacgtt ctgcttgccg aagttatccg
    51721 ggtcgagcgg atcgcgggaa accacgcacg ctttgtcgag atagaaaccg ccatcgccat
    51781 cgtttaaacg aaacagcggc aggatatcga acaggttgat ctcgtcgcca tcaacggtgt
    51841 tctgcgccca ggctggattg gcgcggcgct ccggggcaat cgggaagtta tcccagcggc
    51901 ggataaactc atcaatctgc tttttaaccg gggcatttgg cggcaggccg agggaaatcg
    51961 cgtggttctg ccaggaaccg atggtgttca tcgccacgcg ggcatcggta aagccgcgaa
    52021 tattatcaaa ccacagcgcg ggcgcgccgt cgccgatacg cccggtggcg ttagctgctg
    52081 ctgccagatc cggctcggcg ttcacttctt cgctgatttt cagtaactgg ccgtggtcat
    52141 caagcgcctg taaaaagctg cgtaaatcat caaatgccat tattcattct cctgagaaaa
    52201 attccgggcc tgcggcaatc cttgccagcg cctggcgtgg ggatgttcga ggccaaattg
    52261 atccagcacg cgggctacca catggtggac aatgtcatct accgtttcgg gatggttata
    52321 aaaggcaggc atcggcggca ccatcgccac gcccatgcgt gaaagtgcga gcatattttc
    52381 gagatggatg gtgctaagcg gcatttcacg cggcaccagc accagtttgc ggccttcttt
    52441 gagcacgacg tccgccgcgc gccctaccag gccatcggcg taaccagcgc ggataccggc
    52501 gagcgttttc atactgcacg gaataacgat catgccgtcg gtacgaaaag aaccggatga
    52561 gatgatcgcc gcctgatccg ccgggttatg gctgaagtct gcgagggcag caacatcgcg
    52621 agcgctgtaa ggcgtttcca gttcaatggt ggttttcgcc cacttcgaca tcaccagatg
    52681 agtctcgaca ttcggcatct cccgcagcgc ttgcagtaat gccacaccaa gaggcgcacc
    52741 ggtagcccct gtcatcccga cgatcagttt cattgcttat cctcttaatt gttcgtatac
    52801 gaacgttatt attaacatac tccttcattt ccacagcggc aaggattgaa aagtaacaac
    52861 atcttcttcg tcactaaaaa accgctatga tagcgacaga gaattagccc ggagtttaca
    52921 tggcgttacg aaataaagcg ttccatcagt tacgacagct ttttcagcag cacacggctc
    52981 gctggcagca cgagttacct gacctgacca aaccacagta cgcggtgatg cgcgccattg
    53041 ctgataaacc tggtatcgaa caggtggcac tgatagaagc agctgtcagc accaaagcaa
    53101 cgttagcaga aatgttggca agaatggaaa atcgtggctt agtcagacgt gaacatgacc
    53161 ccgctgataa gcggcgacgc tttgtctggt tgactgccga aggcgaaaag atacttgctg
    53221 cggcgatacc cattggtgac agcgtggatg aggaattttt ggggcgtttg agtggagaag
    53281 agcaggagct gtttatgcag ctggtgcgca agatgatgaa cgcatagggg gcaaatttcc
    53341 ggatgcgacg ctatcgcgtc ttatccagac tacaaataca ccgactcacc aaccgaaaga
    53401 aaaaaggcca gcctcgcttg agactggcct ttctgacaga tgcttactta ctcgcggaac
    53461 agcgcttcga tattcagccc ctgcgtttgc aggatttcgc gcaaacggcg caggccttca
    53521 acctgaatct ggcgaacacg ttcacgggtg aggccaattt cacgacctac atcttccagt
    53581 gttgccgctt cgtaccccag caaaccgaat cgacgtgcca gcacttcacg ctgtttggcg
    53641 ttcagctcga acagccattt gacgatgctc tgcttcatat cgtcatcttg cgtggtatct
    53701 tccggaccgt tctctttttc atcggccagg atgtccagca acgctttttc ggaatcacca
    53761 cccagcgggg tgtctaccga ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca
    53821 tcaactggct tatccagttg ctctgcgatc tcttccgcac ttggttcatg gtccagctta
    53881 tgggacaact cacgtgcggt tcgcaggtaa acgttcagct cctttacgat gtgaatcggc
    53941 aaacgaatag tacgggtttg gttcataatc gcccgttcaa tcgtctggcg aatccaccag
    54001 gttgcgtatg ttgagaagcg gaaaccacgt tccgggtcaa acttctctac cgcgcggatc
    54061 agccccaggt tgccctcttc gataaggtcc agcaacgcca gaccacgatt gccataacgg
    54121 cgggcaattt ttaccaccag acgcaagtta ctctcgatca tccggcggcg agaggcgaca
    54181 tctccacgca gtgcgcgacg cgcaaaataa acttcttctt cggccgttaa cagtggtgaa
    54241 taaccaatct caccaaggta aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc
    54301 gataacagtt cctcttcggc caaatcgtta tcactgggtt cctcttctac taaggccttt
    54361 tcgtcaaaaa cctcaactcc gttctcatca aattccgcat cttcatttaa atcatgaact
    54421 ttcagcgtat tctgactcat aaggtggctc ctacccgtga tcccttgacg gaacattcaa
    54481 gcaaaagcct ggttccgccg atttatcgct gcggcaaata acgcagcggg tttacggatt
    54541 tccccttgta acgaatttca aaatgcaagc gtgttgaact ggttccggtg ctacccatgg
    54601 ttgctatttt ttgccccgcc ttcacttctt gttgttcccg gaccagcatt gtgtcgttat
    54661 gggcgtaggc actcaggtaa tcatcattat gtttgatgat aatcagatta ccgtagccgc
    54721 gcagcgcgtt accggcataa acaacgcggc catctgcggt cgcgataatt gcctgtcctt
    54781 tgctgcctgc gatatcaatc cccttgttgc ccccctcaga agcgccaaag gtttcgatca
    54841 ctttgccctc agtcggccag cgccaggtgg agataggcgt actggttgat gtactgctga
    54901 cagttggctc ggttgtgctt gctgttggta ccgttacagg cgctgtgacc gtggtcgcag
    54961 ctggcttgtt gttcggcaac attttgttag cactctgttc acccgaagat tcagaatacg
    55021 taattgtcgg ttgcgacgca acagcaacgg tggaattttg tgcaggcttg atcacaactc
    55081 cttgctctgc tgcgtcggcc tgggtaatgg catttccgcc agtgattggc gtaccggaag
    55141 cattacccac ctgcaaggtc tgaccaacgt tcagcgcgta tggtgcctga atattgttgc
    55201 gctgagcaag gtcacggaaa tcgttgccag taatccaggc gatatagaaa agtgtgtcgc
    55261 cttttttcac ggtataggta ctgccgctat aactgccttt cggaatgttc ccatactgac
    55321 ggttatagac gatgcgtccg ttttccatct gtaccggctg ctgagctact ggctgcactg
    55381 gctggatttg cggttgttga gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg
    55441 gttgctgggc tgtagacgtc gtccccattt tcggcggcgg cgtaatcaac ataccagaat
    55501 tggtatttgc aggcgcattg ccattaacgg agctgaccgg tgccggtgga tttgaagtgt
    55561 cagaacagcc tgccagccat agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga
    55621 attttgggct tcccgcgctc atttatcccc caggaaaaat tggttaataa ccagtgacat
    55681 aattaccgtg caaggcaccc tactgaacac tggaaaagat gttcacgata cgctgacctg
    55741 cggcaaaata accaggaaaa atccaggtat ttcctcacgt tttaagccag ctcacccttc
    55801 actaaaggta caaagcgcac ggcctccacg gtatcgataa taaattcgcc tccccgacga
    55861 cgcacccgtt tcaaatactg gtgctcctcc cctacgggta agacgagaat cccgccttcg
    55921 tccagctgcg tcattagcgc agttggaatt tccggcggtg ccgccgtaac aatgatagcg
    55981 tcaaacggcg cgcgtgcctg ccaaccttgc catccatcgc catgacgggt tgaaacatta
    56041 tgtaaatcaa gatttttcag gcggcgacgc gcctgccact gcaaaccttt aatccgttcg
    56101 accgagcaaa catgctggac aagatgcgcc aggattgccg tttgatatcc cgaaccagtg
    56161 ccaatttcca gcacccgcga ctgcggcgtc agctcgagta attcggtcat tcgcgccacc
    56221 atatacggct gcgaaattgt ctgcccctga cctatcggca aggcgatatt gtcccaggct
    56281 ttttgttcaa acgcttcatc aacgaatttt tcacgcggca cggcggcaag tgcattcagc
    56341 acctgctcat cctgaatacc ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg
    56401 cttaccattg cgtgccaact cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc
    56461 tatgcgcagt taaatccaca tgcagcggcg tgatggagac atagccctca tctaccgcag
    56521 caaaatcggt ccccggaccg gcatcacatt taccgcccgg cgggccaatc cagtacagcg
    56581 tattgccgcg cggatcttgc tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc
    56641 gcgtcacgcg aatacctttg atttgatcca agggtaaatc cggaacgtta atattaagaa
    56701 tacgcccggt gcgcaacggc tctttacaca gtgcgcgcaa aattgaacag gttaccgccg
    56761 cggcagtgtc gtaatgttta tgcccgtcaa gcgagacggc aagcgccgga aaacctaaat
    56821 gacggccttc catcgcggcg gctaccgtac cggaataaat aacatcatcc cccagattcg
    56881 gcccggcgtt aattccggac acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc
    56941 caagatagac gcaatcggtc ggggttccca tttgcacagc aatatcacca ttttcaaagg
    57001 taaacgtgcg cagggaggat tccagtgtca gagaatttga agcgccgctg cggttacgat
    57061 cgggggcgac cacctgaacg tcagcaaact cacgcaaggc tttcgccagc gtttgtatac
    57121 cgggtgcatg taccccgtca tcattactca gcaatatgcg cataatcacc tgttgtgttg
    57181 ataagttccc tgacaacgct ggttgcaaaa ctacccgccg gaagccagaa acggatctct
    57241 acggtgacgt catcccacca attccagctt aattgttgcg gatacagcag catcgctctg
    57301 cgcgcggctt caactttttc gcgcaccagt aaagcttgta attcagtttc tgcggcgaca
    57361 gctgcttgtt cgaatgccag cgcttcacgc tgagttcccc attcgccact gcccggcaat
    57421 gcggcggtta tcatcagctc tttatcgtta acgcgacgct gtaattccgc cagttcttcg
    57481 gtggttgcga caaaccagct accacgtccg gctaattgta gcgcatcgcc gtcaacaact
    57541 tgattaacgt ctgctttttt gaggcgctca gcaacaatct gattaaacaa cgcactgcgg
    57601 gctgccgaca accaaaaact ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc
    57661 cagcgctgcg ccccctgcaa gttgctaccg ccaatcccaa aacgttgggc accgaagtag
    57721 ttcggtacac cttttacgca aatatcgttc agacgttgtt caacgtcatc gcgattgctc
    57781 acttcgcgca gaaccagggt aaaggcgttt cctttcagcg cccctaaacg cagcttgcgc
    57841 ttgtgccgcg catactccag cacctggcag ccttccagtt gaaaggcgct cagatcgggc
    57901 atttccttgc ccggcacgcg agcgcataac cactgttccg taacagcatg tttgtctttt
    57961 tgcccagcga agctgacttc acgggcatga attttcagga atttcgccag tgcatccgcc
    58021 acaaaacggg tattgcagcc gtttttgagg attctaacca gaatatgctc accttcacca
    58081 tcaggctcaa agcccaaatc ttccaccacc acaaagtctt ccggattggc tttcagcagc
    58141 ccggtgcctt gcggtttacc gtggaggtaa gtgagattat caaactcaat cattttgttg
    58201 ccttaatgag tagcgccacc gcttcacagg caatcccttc cccacgtccg gtaaatccca
    58261 gtttttccgt agtagtggct ttcacgttaa catcatccat atggcagccg agatcttcgg
    58321 caataaacac gcgcatttgt ggaatgtgcg gcaacatctt cggtgcctga gcgatgatag
    58381 tgacatcgac gttgccaagg gtataaccct tcgcctgaat acgacgccag gcttcgcgta
    58441 gcagctcgcg gctatcggca cctttaaatg ccggatcggt atccgggaac agcttgccga
    58501 tatcccccag cgccgccgcg ccaagcaatg catcggtcaa cgcatggagc gccacgtcgc
    58561 catcagaatg cgccagcaat cctttttcgt aaggaatgcg tacgccacca atgataattg
    58621 ggccttcacc gccaaaggca tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc
    58681 ctgatggatg gttcgggtga ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac
    58741 tttaatgtta tccgcacggc cttcgaccaa ctgaggatgg aatccgcaat attccagcgc
    58801 cgaggcttcg tcggtaatag tcgcgccttc atttagagcg cgcgtcagac agtcatgtaa
    58861 cagctcacga gggaaaaatt gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt
    58921 atgagcaatg gcatttttgc ccggttcggc acgtttcata gtatcgcgca ctggtgcggc
    58981 gaggatcccc cccgtgcggc tggtttcgct caacgccaac aatcgcgcga ggtcatcctg
    59041 atgcaaacaa ggacgagcgg cgtcatgcac caatacccac tgcgcgtcgc cagcggcttt
    59101 cagacctgcc agcacggaat cggcacgctc atcaccgcca tctacaacgg tgatttgcgg
    59161 atgattcgcc agaggaagtt gtgcaaaacg gctatcgcca ggacttatgg caatgacgac
    59221 tcgtttcacc cggggatgcg ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt
    59281 accgattgag agatattgct taggacattc cgtttgcatt cgacggccaa atccggccgc
    59341 cggaaccacg gcgcaaacat ccaaatgagt ggttgccatg ttaattcccg ggctgattta
    59401 tcgattgttt tgccccgcag actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa
    59461 agtttcgccc ggcctggtca tgctgagttc attacgcgca cgctcttcga gcgcctcctg
    59521 gccgccattg agatcgtcaa tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc
    59581 gtttgtagct tgctgtgccg ccacatcatc attgacgcgg gtatagtcat gtataccgtt
    59641 cttaccgaac cacagcgaat actgtagcca gaccagaata gccagcaaca gcagcgttag
    59701 tttacccatc ctgccccctg aaaaacggca tcatcatccc atgcatccga agacgactct
    59761 acatcctctg ttggggatac cgcgacaacg cgggcaaatg taccacattt gtccattgtt
    59821 acgtataccc agggcgtgca gaacataatc tcattattag ttacggtttg aattatgaac
    59881 agaggagacg ggaaagtaca aattagccca gtagccacat aaacagtgcg ccaaacataa
    59941 tgcctactgt caccagggtg aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat
    60001 gcagcgcaat ccccaccact accgcgacgg gcatcagcgc cagaaagaaa ggccaggtgt
    60061 agataaagaa gaacagcgtg ttagagccat aaatcaacat cggcatcgcc agcgcaaata
    60121 accaggagat aaaaccgacc acggcaccag gcagtgacca tgtggtttct tcatcctcag
    60181 taaggctgtc gttatttgtt agtgtaatgt tatggctatt acgcatattt gatcctgtta
    60241 ctttgacgaa ccgggcatgg aaacccggtg gtgtctcagg atctgataat atcgttctgt
    60301 ctcaacagat ctaataattg ctgtaccaaa tttgttacta attgttcacc attgagatga
    60361 atttctgccg attcaggcgc ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca
    60421 ccggcacgcg ctttcttata taagcctttt ggatcgcggg cttcgcaaat cgccagcggc
    60481 gtatcgacaa acacttcgat aaagcgccct tctcctacgc gttcgcgaac catctggcgt
    60541 tcggcgcggt gtggcgagat aaatgcggtc agcactacca gtccggcttc aaccatcaaa
    60601 ttcgccactt caccgacgcg acggatattc tctttacgat cggtatcgct aaaaccgaga
    60661 tcgctgcata atccgtggcg aacattatcg ccatccagca gatacgtact gacgccgagt
    60721 ttatgtaacg cctcctccag cgccccggcg accgttgatt taccggaccc ggagaggccg
    60781 gtaaaccaca gcactacacc acgatgaccg tggtgtagct cgcgttgttg cacagtgacc
    60841 ggatggctat gccagacgac gttttcgtca tgcagcgcca ttatttatcc cccagcaaat
    60901 cgcgcgcgcc ccagtgcgga aagtggcgac gaaccagagc attcaattcc agttcgaatg
    60961 cactgaattc agatggcgca gcagttgcct ggctaactgg ctcgtgcacc ataccggcac
    61021 ccacggtcac attgctcagg cgatcgataa aaatcagccc acccgtcacc ggattttgtt
    61081 gataacgatc taacaccagc ggctcgtcaa aagtgagatc cacgaggccg atcccattca
    61141 gtggcaggtt ttcaacttca cgctgggtaa ggttattaat atcaacctga tagcgaatgc
    61201 catcaacacg cgcgcgcgtc ttcttaccgg caattttgat gtcgtaactc tgccctggag
    61261 aaagcggctg ttccgccatc cataccacat ccaccgacgc gctctgcacc gccggtaacg
    61321 cttcgtctgc cgccagcagc agatcgccac ggctgatgtc gatctcatcc gtcagcacca
    61381 gggtgatcgc ttctccggca aaggcttctt cgcgatcacc atcaaaagtc acgatccgcg
    61441 cgacgtttga ttccacacca gagggcagca ctttgacacg ttgcccgact tccacgcgac
    61501 cggatgccag cgttccggcg taaccacgaa aatcgagatt cgggcgatta acgtactgca
    61561 ccgggaagcg cattggctga gcatccacca ctcgctggat ctccacggtt tccagcactt
    61621 cgagcagtgt cggaccgctg taccacggca tactttcact ttgcgatgcc acgttgtcgc
    61681 cttccagtgc agagagcggc acaaagcgga tatccagatt acccggcagc tgcccggcaa
    61741 aggtcaaata atcttcacga atacgggtga acgtctcttc actgtaatcc accagatcca
    61801 ttttgttgat cgccacgacc agatgtttgc tccccaacag tgtggagata aaactgtgac
    61861 gacgggtttg atcgagcacg cctttacggg catcgatcag taagatcgcc agttcacatg
    61921 tcgatgcgcc agtcgccata ttgcgggtgt actgctcgtg ccctggggtg tcggcgataa
    61981 taaatttacg cttctcggta gagaaatagc ggtaggccac gtcaatggtg atgccctgtt
    62041 cgcgctcagc ttgcaggccg tccaccagca gagccagatc cagcttttcg ccctgggtgc
    62101 cgtgacgctt actgtcgtta tgcagcgatg agagctgatc ttcgtagatt tggcgggtat
    62161 cgtgcagcag acgaccaatc agagtacttt tgccgtcatc gacgctacca caggtcagaa
    62221 aacgcagcag gcttttatgt tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg
    62281 cgatttgttg tgcaagtgcg gtgttcatct taaaaatacc cctgacgttt tttcagctcc
    62341 atagaccccg cctggtcgcg gtcaatcacg cggccctgac gttcactggt ggtggaaacc
    62401 agcatctctt cgatgatttc cggcagtgtt tgtgcatttg actccaccgc accggtcagc
    62461 ggccagcagc ccagcgtacg gaaacgcacc atccgttttt taatcacttc gcccggttgc
    62521 aggtcgatac ggttgtcatc aatcatcatc aacataccgt cgcgttccag aaccggacgt
    62581 tccgcagcga gatatagcgg aacaatgtcg atattttcca gccagatgta ttgccagata
    62641 tcctgctcgg tccagttaga gagcgggaag acgcggatgc tttcgccttt gttaatttgc
    62701 ccgttgtagt tgtgccacag ctccgggcgc tgatttttcg gatcccagcg atggaagcgg
    62761 tcacggaaag agtaaattcg ctctttagcg cgggatttct cttcgtcacg gcgcgcacca
    62821 ccgaaggcgg catcaaaacc gtatttgttc agcgcctgtt tcaggccttc agttttcata
    62881 atatcggtat gtttcgcgct gccgtgcacg aatggattaa tccccatcgc cacgccttcc
    62941 gggtttttat gcaccagcag ttcgcagccg taggctttag cagtacgatc gcggaactca
    63001 tacatctcgc ggaatttcca gccggtatcg acatgcagca acgggaaagg cagcgtacct
    63061 ggataaaacg ccttgcgcgc cagatgcagc atgacgctgg aatctttacc gatagagtag
    63121 agcatcaccg gatttgagaa ttctgccgcc acctcgcgaa taatgtggat gctttccgcc
    63181 tccagttgcc gcaggtgagt aagtcgtatt tgatccataa ccgttccttt gcaataccgc
    63241 tattttcttg ccatcagatg tttcgactat agggagcgta agagaacgaa tgaaattacc
    63301 aattagaatg agtagttcct taacggaata acgatttggc aaagctaata tcaaaaagtg
    63361 cttaaggcac cggatttcgg gcgtttagga agatttgaaa ttgttttagc gcagcggcag
    63421 tttcatacta tggcggtaaa aaaatttgca tggtatttaa ggactcacta tgttttccgc
    63481 attgcgccac cgtaccgctg ccctggcgct cggcgtatgc tttattctcc ccgtacacgc
    63541 ctcgtcacct aaacctggcg attttgctaa tactcaggca cgacatattg ctactttctt
    63601 tccgggacgc atgaccggaa ctcctgcaga aatgttatct gccgattata ttcgccaaca
    63661 gtttcagcaa atgggttatc gcagtgatat tcggacattt aatagtcggt atatttatac
    63721 cgcccgcgat aatcgcaaga gctggcataa cgtgacggga agtacggtga ttgccgctca
    63781 tgaaggcaaa gcgccgcagc agatcatcat tatggcgcat ctggatactt acgccccgct
    63841 gagcgatgct gacgccgatg ccaatctcgg cgggcttacg ttacaaggaa tggatgataa
    63901 cgccgcaggt ttaggtgtca tgctggaatt ggcagaacgc ctgaaaaata cgcctaccga
    63961 gtatggtatt cgatttgtgg cgaccagcgg cgaagaggaa gggaaattag gcgcagagaa
    64021 tttactcaag cggatgagtg acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga
    64081 taacttaatt gttggcgata aattgtattt caacagcggt gtaaaaaccc ctgaagcagt
    64141 aaggaaatta acgcgcgaca gggcgctggc aattgcgcgt agtcatggaa ttgccgcaac
    64201 gaccaatccg ggtttgaata aaaattatcc gaaaggcact gggtgttgta atgacgcaga
    64261 aatattcgac aaagcgggca ttgctgtact ttcggtggaa gcgactaact ggaatcttgg
    64321 gaataaggat ggttatcagc aacgcgcaaa aacacctgcc ttcccggcgg gaaatagctg
    64381 gcatgacgta agactggata atcaacaaca tattgataag gcacttcctg gaagaataga
    64441 acgtcgctgc cgtgatgtta tgcggataat gctaccgctg gtgaaggagc tggcgaaggc
    64501 gtcttgatgg gtttgaaaat gggagctggg agttctaccg caggggcggg gaattctaag
    64561 tgatatccat catcgcatcc agtgcgcccg gtttatcccc gctgacgcgg ggaacacaaa
    64621 accaaacttc tccataaatt ccatagccgc ggtttatccc cgctggcgcg gggaacactc
    64681 ttcgcgggta atcaatgatg attcagtttc cggtttatcc ccgctggcgc ggggaacacg
    64741 aacgcaatat cacggcgttc tgcggcctcg gcggtttatc cccgctggcg cggggaacac
    64801 caaaatatta cgagcttcgt caggccatgg accggtttat ccccgctggc gcggggaaca
    64861 cgtcgggccg ggtaagtgaa cctgtcggtt ttccggttta tccccgctgg cgcggggaac
    64921 acactaagca tacataactg tttttaaaca aatttattcc gcatcaacaa tctaccaact
    64981 aaattaaaac atttccttat ttttaaagaa cggataactt attgattatc aacaggaaga
    65041 aaagaaacca aacgtaaccc atccaaatcc accggaatac gtctgttttc tccccaggtc
    65101 tgaaattcaa aacccgactc ggtattggtc gcccaggcca tcaccacatt tccgcaacca
    65161 gccagttggg taatttgctg ccagatcatc tcccgaatac gttttgatgt atcaccaaca
    65221 tacacaccgg cacgcacttc cagtagccag attgcgagcc gtccacgtaa gcgcggcggg
    65281 acattttctg taacaaccac gaccatgctc atcagccgcg tcctcgatga ccgctatcgc
    65341 ccaacgtttc aggttcgggg atggctggcg gtaacatatc cggcgcaggt tgtggcggtt
    65401 caatttcacc tgcggcaagg acttcctcaa ttaacggtat taatttgccc gttaacttag
    65461 tgctacggaa aatatcgcga caggctaatc tgacttcttt atcaggttct gcgggttgcc
    65521 tcgctgctat ttcaaatgcc tttggcacaa ccgaatcaaa tttaatgata tcggctatgt
    65581 cataaacaaa tgaaagcggt ttgccactat ggataaaccc aatagcgggc gcatatcccg
    65641 cggctaatac tgccgcttca gatataccgt acagacatga tgtagcagca ctgatgcagc
    65701 gattcacaac atcgcctttt tcccagtctt taggatcgta tttgcgacca ttccatttca
    65761 caccatattg tttcgccagt aatgcatacg tctggcgaac gcgagatccc tcaattccac
    65821 gtaactgctc cactgaacgg cgagctggct gtggttcacg aaaacgtaat tcatacattt
    65881 tgcgcaccac cttcaaccgt agatcttccg ttaaagccag ctttgcctgg tagagtaatt
    65941 tatctgcccg cgccccaccg ggttgtccgg aggagtaaac gcgaacgccc gcttcaccga
    66001 cccagaccag cagtgttccc accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg
    66061 ttcctggttc gagcataatg caggcgaccg atcccaccgg aatatgcgtt cggatccctg
    66121 ttttgtcgat cagcacgaaa gcgccgtcca gtacgtcgat ctgaccgtac tggaggaaga
    66181 tcatggaggt gcgatctttt aatgggatcg ggctcagtgg tacaaacgtc acgcctctgc
    66241 tccgggtttg atcagcatca gaccacagcc gaacgcccgg cttttaccat acccctggct
    66301 caggcgttgc acaaataacc caggatcggt gaccgtaagc atccccgtat aatccacgct
    66361 actgaactgg atcagttgcc gggagttttc ccgccgcagt tgctgttgtc ggtaggcatc
    66421 aaccgaggta tccagcagtg taaaaccgct tctctccccc tgcgctgcca gccagtccag
    66481 cgccgcctgt tgttgatgca accagacatc acttccttcc gccggccccc tcacctgccg
    66541 tttcgcctcc atcagcagat cgtggcgctt gcccgcttta cagattgttg gattcgcccg
    66601 caggttgaaa caaagttgtt gtccggtaca cagctcgggc gcaaatgacc ggcattctat
    66661 ggtgaacgta tcactttccg ccgggcgttc ctgcgacagt acaaaaaagc gaaacgcgcc
    66721 ctggagttct tcccgacgat aaagaaattg cctttctttg ccgccaggga agagatccca
    66781 cagccactga tgcatcacat attcaccgcg atccaccaga tgcagcaact gcgcaggcga
    66841 aagctggccg gtatgtaagg ttattcttga gaggtacatg gttcctcctt gctgagccac
    66901 ggcccctgat tgacggtgcg ttccccaaac agccactgct gacgatttaa aggaacatcc
    66961 cggcggcgta atattttgct cgcgaccagg ccgtcgtgtt tcccttccca ccagcattca
    67021 tcctgaagtt tcgggagtga gactttcagt ttatggaaat gatcctgata ctgttggtat
    67081 gcattacata aggcatcaca cgcgttgcct tcgagcagta acggcgcaag aggtaatgcc
    67141 agcggatgac tttttcgccc cagataaagc ggaaaaactg gatggcgtaa accgtcctgc
    67201 aactgttcga ggctgtaagg cgcatcggct gttgttgcca ccgccaccat ccaccaggca
    67261 tcggtgtagt agtcgcgccg ggagataatc gcgctcaaaa gctcagggtc gctcaactct
    67321 tcgcgacggc tgaaataacg tgctttacgc acctcttttg gcatctggac cgtgtgataa
    67381 tcccgtgccc agcgcgggtt acggctggcg caaaccacca gtgaatagtg gcgattaaac
    67441 gcgtttaagc gttcggtatc atcacgccga atccctaccc cggcagccag tagccccagc
    67501 aatgctgagc gcgaaggaag ttcatgggta tgacgaactt cacctggagc atcgaccccc
    67561 caggacgcca tcggcccatg aagctgaaaa atcagatatt ggctcataag ccgcccctta
    67621 cgcgcagata aagtccagca cgtccttcat gcttccctgt ttgttcatca cgtcaaaact
    67681 tgcgcattcg gtcttctgtt cgtagaccgt attcatgttt tcgcgaagcg ttgtaatacg
    67741 ctgcaccgcc acatctaact gacgtgtgcc attaatgggt tcatagaaag ccgccgccag
    67801 agagcgtggt tgatcggtgc ctttttctgc cagcgcccag gaggcgtagg cacggctggc
    67861 aaagctgttc tgtttgccgg ttggggagac tttaagtgcg gcttccgtaa aggcacgtat
    67921 ggtctgatta gctaacgctt cgtcaccgcc aaggttttcg accagcagat ctttatcaat
    67981 gcagatatag gtgtagaaca gtgcagaacc gaatccggtt tcaccaagat gcccggcacc
    68041 ggcatcttca gaagcctggc gcaaatcatc aacggcggtg aaaaaatcat cttcgacaat
    68101 cgtttcactg acaccaaatg cgtgcgctac ctggcaggcg gcttcaacat taaactcggg
    68161 tttattcgcc agcatacgac caaacatggc aatatctacc gccatacggt ctttgcgtaa
    68221 caacgcgaga tcttcctctt ttggcgcgcg cttttcttct gcaagctgat gggccagcgc
    68281 ttttacggcg tcaaattctg ccgggctgat atggactaac tgttcagttt cggcgttagt
    68341 gagcggatct tttggttttt tgtcattttt agctttccca agataatcag caatttttgc
    68401 cgaccattcg atggcttttt tatcttcaat ccctttctca atcaggatag ttgccgcctc
    68461 acgcgcaata cgcccactgc gaataccaat atggcccgcc agtgcctgtt caaaaagtgc
    68521 agaagtgcgc cacgcacgtt tcagactttg cgaggaaacg cgcagacgcg ttgctccacc
    68581 cagcaccaca gtttttggcg caccggtatc atcacggtta aggttggcag cagggtaagc
    68641 ggttaacaaa tgaagctgaa taaatgttgt catgagatag tcctttattg attttgttcg
    68701 ttatcggcgt cgccagcctg gtaatattcc agcgcccagc ggatacggat aaattcggtt
    68761 ggccgctgct ggcggcggtg atgattgagc aggtcgtcgc tctcctggca ccaacggaag
    68821 accccctctg ccagagagtc aagattgacg gaaccgttta ataatctgac tgcgcggcgt
    68881 aactggcgca gtaattcatc cggtgttttt actgccgaca gacgagtaaa acgccccttt
    68941 gacattaccg ccgccagctg cgcggcaaaa ggctgccgtt catcaatagc ttttacattg
    69001 gcgctaactg cggcgaccag cgccagtgcg gtaaaacgcc actcttgtgc ttcaattttc
    69061 cacaaagtgt gggtctgcat cagcagagaa cgaaaccctt ctgacaagca aacatcatta
    69121 accgttgtgc tacgccgcag acttgcacga ttgccgcgtt tagcctgcaa ctcttcgcgc
    69181 catttacgca gcgtggcttt gtgctcctct tttacaatac tcattcagca gcctcctgct
    69241 ttttctccct ggcggcttta gcactttgct tctccgccga tgttgtgaaa tatttcttgc
    69301 gcgcggtcat aacgcgtttc aaatcaacag gctcatatgg attggtgaat acacgctcgt
    69361 caaaatcctg acgcgcgaat aaccaaattt ccttttgcca tttgccgagt aattcgtccg
    69421 catcctgacc ttcttcaatt tggcgcacta acctcaggaa gcgatgctga gttttgttcc
    69481 agaagtcgat atccacaaaa ctgaaatcac cccgtgcacc ttttggatcg gagaaccacg
    69541 cttctttcaa tgcacttcgt aacagactta gaatccgtga agccgtttgc gcagccagac
    69601 gcagtttcgg tatctggcct tcttttttct tgagcagcag cgggaaatgg tgttcgtacc
    69661 aacagcgcgc tttcatgttg tcgaaatcat agccaaatcc ccacaggcct acttttgcct
    69721 gtttcagact gctggcatta aagagtttca ccaccagcgc gggaagttcc gtattgtttt
    69781 ctgatttacc cgtttcgata aggcctaacc agtcgcgcca gattaaaccg cccggttgtg
    69841 gtttgaccga gtaaaactcg ccaccctctt taagtggtat acggtaaggc gttaagggat
    69901 gctgccacat ggcataattc gcaccgtaat ttttggtcgt catcaggctc aggagcgcgt
    69961 cgctctgttc accgcaaata tcgcagttgc cgactgtcgt ggtattaaaa tcaatacgaa
    70021 tacggcgcgg cattccccag taagcctgga gtttattgac ctgatcatgg gttaccaccg
    70081 caccggccag ttcgctggtt cgcgtcgggc caagccaggg gaaaaccaga tcgtcaaatt
    70141 ttttgggtag cggtaagtcg gcttcatcct gcggcatcac attgggccac agtttgcgcc
    70201 acaagggcgt ttgttgattg ccttgatatt cctgtaattc aatcagagtg gtcatcggcc
    70261 caccgccgcg caaaccggtg cgatagcctt tgccgcctga tggcgcattt aactgtaggg
    70321 agaacagagc taacgcggaa caatgagggc atacgtgttc agtcacgcca cgcttaataa
    70381 agtggtcttt attaaacttc gttgtttgag cgccgggaat ctcaggcagt agcgaagcga
    70441 cctgaacttt atctcccttg agcgcctcga aatcctgcat aaatgaaggg gaatctgccc
    70501 caaactggaa agcgtgttct aaggacagca atgcttcccg tagcttttca gcctccagtc
    70561 cgtcttgcca gatatcatcc caacgaccgt gatttttggg cgcgaagctg gtttgtagta
    70621 gccccagcaa aaactgccag gccgccccct ggagatctgc ccgcggcgca gcgatatcga
    70681 caacattttc atcagccaga tcgactggtg ccagcttgcc tgtcgttccg tctttaaaac
    70741 gaacaggcaa ccacggggtt gtcagaagtg aaaacgagtt catacgcatc ctctcgtaaa
    70801 aaagaccatc ccaggtcggt tgtccatctt tccatccttt tcggtgtatc aactattcag
    70861 agagtcctgt cgcagacatc tcaaccaacc ttgccaatca atccttccgt agccgaatac
    70921 ccgcaacttt cgtcatcgtt gactaaaatc acgtttgcca tctccggatc ttgtcgctgt
    70981 tcaatgcacc acagcctgaa cgcctctccc tccagtaaag aaaactcatc ccgatgtttt
    71041 ttccaccagc ttcggcgcac tctgacaacg ctcatttccc atgcgtgagc accggtggca
    71101 taaggcttca ccacaccgtc aatacaggta gccagccaca gggaaacaga ctcctcagcc
    71161 agacgtgtcg acagtttttc cgggaggtaa tcgttgatat tggcggcata gccgggcctg
    71221 aagttcagca caaacttttt agccattgcg cgatcgcagt aatatttgcc cacttgctcc
    71281 tgctcgctgc gggcaaatcc ttccggcatt accacgtcct caccgtagac ggattcaata
    71341 agaagccggg ctgcgtgtgg catctgaata gcaccttgct cacgcagcac acgctgcgtc
    71401 agccagattc gcccatgatc gggatagaca aatgcactgt tacgcatggc actgccaaac
    71461 cattcgtcgc caggagagtc gtcccagacg ggagccagaa tcagcaattc agggggggaa
    71521 cgctcgtcct ttccgtcacg ctttaactga ccattaatat cgcggatatg ccgctgtaat
    71581 cgtcctgctc gctgaatcag caaatcaaca ggggccaggt cggagatcat ttcgtccagg
    71641 tcaatatcca cgctttgctc aattacttgt gtacaaacaa tgacttgcga cgctctctgt
    71701 aaactacaat atttaccaaa gcgtgccagc gtttccgttt caattcgctg gcgatcgcta
    71761 aaagcaaagc ggctatgaaa aagtgaaagg ctggaagcag gaatgacgcc gcgggcaagc
    71821 agctgacgat gaaccttaat agcgtcatcg acagaattcc ggatccaggc gatgcatttc
    71881 ccctgactta ccgccgattc gatacgcgca atacactctt gttcactatg aagccaaccc
    71941 acgctgacgc tacgctcaac atctttgcgc gtcgctaccc ggtgtgagtt cacatcggat
    72001 ttcgtgacat gcgtcagcca ggggtaatca tccttttcca ggaacggagc ttcttgctgg
    72061 ccctctgtac cccgcgcaaa ggcagcgacg agtttgtcgc gctgctgttg agataacgtg
    72121 gcagaaagca aaataacgct gtttccgcca cgcgcctgcc gctcgatcaa cccttcaaga
    72181 atgcacgaca tgtaagcgtc acaggcgtgg atctcatcag ccagcaggat tttgttactc
    72241 agccccagaa gccgcagatt attatgtttg aacggcatca ccgccatcat cgcctgatcc
    72301 agcgtgccga cgccaatttc agccagcaac gccttcttgt tactgtcggc aaaccaggcc
    72361 gcacatccct gactgaacgt ttgttcatcc ggttcttctg acccgactaa atcaccagac
    72421 cagagtgatt cattaaagcg gtccattaat gtgcgggcac tatgtgccag caccaggctg
    72481 gggcgggatt ctggcgaata gaaagcaagc caggttttga ccagccgatc gtacatggca
    72541 ttggccgttg ccattgttgg cagaccaaaa aacagcccct gagctttccc cgcagctatc
    72601 aacctgtgcg ccaggataag cgccgcttct gttttacctg cgccagtcac atcttccaga
    72661 ataaataact gcggtccttg ctggctgata tccagatcca gcaccttttg ctgtaacggt
    72721 gtcggattct caataaaagg aaacagcgta ttaattccgt tgaaaggcgc gacttttgct
    72781 tttagaggaa agacggttaa ggcgttttga gcctgaatta aagttttctg ccagtaacct
    72841 ttaatatcca ttggatgtgc gacgcgtgga aaaaatcgcg ttgacgaacc cgtccagtct
    72901 gcgagcacga cggttgcaga gatataccag gaaaggtgtt ttataagttc aatgccctca
    72961 tcatcatccc agaatgcggg gatctctatg agcggaaaca gtggtttgat ttcaaggaga
    73021 aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat ccaattcatc catacgatca
    73081 gggggctgac catgatgccc tgtcgtcacg ggcatccaca tttctatcac tcgcctaagt
    73141 ttgcgcggag agagcgaaga tgaaggaagt aactcctgac attcacttaa ataatgattc
    73201 cacaaccagt agcccagcgt tgaatgagag attttttcgt aattttttct ggcaccttcc
    73261 tgaatcttga gttcaggggg caggtaaagt tgttgaaaag agcgggcaaa ttttccaata
    73321 tcgtgccagc acagcaacca ggcgaaaaat tgagccgcct gttccttgtc agaaatccct
    73381 aattgacgaa aataatcagc cagcccgaag caatttcttt taaccattaa atagcccatc
    73441 gcggctacat ccagcgaatg ccagcaaaga aggtgatagt cgtcgccccc gtctttctcg
    73501 ccgcgtcggg tttttcccca gaaatcaaa
//
LOCUS       DAZFRJ010000002        70975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000002 DAZFRJ010000000
VERSION     DAZFRJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.002"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..118
                     /gene="rcbA"
                     /locus_tag="ACHKUN_000079"
     CDS             <1..118
                     /gene="rcbA"
                     /locus_tag="ACHKUN_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFO6658410.1"
                     /translation="KLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQSS"
     gene            218..433
                     /gene="xisR"
                     /locus_tag="ACHKUN_000080"
     CDS             218..433
                     /gene="xisR"
                     /locus_tag="ACHKUN_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO6658411.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            435..1670
                     /gene="intR"
                     /locus_tag="ACHKUN_000081"
     CDS             435..1670
                     /gene="intR"
                     /locus_tag="ACHKUN_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6658412.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTLISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNMWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            1722..2657
                     /gene="ttcA"
                     /locus_tag="ACHKUN_000082"
     CDS             1722..2657
                     /gene="ttcA"
                     /locus_tag="ACHKUN_000082"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO6658413.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2786..4159)
                     /gene="dbpA"
                     /locus_tag="ACHKUN_000083"
     CDS             complement(2786..4159)
                     /gene="dbpA"
                     /locus_tag="ACHKUN_000083"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO6658414.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4189..4362)
                     /gene="ynaL"
                     /locus_tag="ACHKUN_000084"
     CDS             complement(4189..4362)
                     /gene="ynaL"
                     /locus_tag="ACHKUN_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO6658415.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4644..5627)
                     /gene="zntB"
                     /locus_tag="ACHKUN_000085"
     CDS             complement(4644..5627)
                     /gene="zntB"
                     /locus_tag="ACHKUN_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO6658416.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5641..5688)
                     /gene="ydaN"
                     /locus_tag="ACHKUN_000086"
     CDS             complement(5641..5688)
                     /gene="ydaN"
                     /locus_tag="ACHKUN_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO6658417.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            5882..7114
                     /gene="dgcM"
                     /locus_tag="ACHKUN_000087"
     CDS             5882..7114
                     /gene="dgcM"
                     /locus_tag="ACHKUN_000087"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO6658418.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7135..7698)
                     /gene="smrA"
                     /locus_tag="ACHKUN_000088"
     CDS             complement(7135..7698)
                     /gene="smrA"
                     /locus_tag="ACHKUN_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO6658419.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8028..8936)
                     /gene="abgR"
                     /locus_tag="ACHKUN_000089"
     CDS             complement(8028..8936)
                     /gene="abgR"
                     /locus_tag="ACHKUN_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6658420.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9112..10422
                     /gene="abgA"
                     /locus_tag="ACHKUN_000090"
     CDS             9112..10422
                     /gene="abgA"
                     /locus_tag="ACHKUN_000090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO6658421.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10422..11867
                     /gene="abgB"
                     /locus_tag="ACHKUN_000091"
     CDS             10422..11867
                     /gene="abgB"
                     /locus_tag="ACHKUN_000091"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO6658422.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            11904..13430
                     /gene="abgT"
                     /locus_tag="ACHKUN_000092"
     CDS             11904..13430
                     /gene="abgT"
                     /locus_tag="ACHKUN_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO6658423.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13441..13956
                     /gene="ogt"
                     /locus_tag="ACHKUN_000093"
     CDS             13441..13956
                     /gene="ogt"
                     /locus_tag="ACHKUN_000093"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO6658424.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14151..14903
                     /gene="fnr"
                     /locus_tag="ACHKUN_000094"
     CDS             14151..14903
                     /gene="fnr"
                     /locus_tag="ACHKUN_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO6658425.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15055..16005
                     /gene="uspE"
                     /locus_tag="ACHKUN_000095"
     CDS             15055..16005
                     /gene="uspE"
                     /locus_tag="ACHKUN_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO6658426.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16055..16312)
                     /gene="ynaJ"
                     /locus_tag="ACHKUN_000096"
     CDS             complement(16055..16312)
                     /gene="ynaJ"
                     /locus_tag="ACHKUN_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO6658427.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16556..17587
                     /gene="ynaI"
                     /locus_tag="ACHKUN_000097"
     CDS             16556..17587
                     /gene="ynaI"
                     /locus_tag="ACHKUN_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO6658428.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(17638..19251)
                     /gene="mppA"
                     /locus_tag="ACHKUN_000098"
     CDS             complement(17638..19251)
                     /gene="mppA"
                     /locus_tag="ACHKUN_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO6658429.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(19588..20487)
                     /gene="pgrR"
                     /locus_tag="ACHKUN_000099"
     CDS             complement(19588..20487)
                     /gene="pgrR"
                     /locus_tag="ACHKUN_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO6658430.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            20625..21545
                     /locus_tag="ACHKUN_000100"
     CDS             20625..21545
                     /locus_tag="ACHKUN_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6658431.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            21545..22477
                     /locus_tag="ACHKUN_000101"
     CDS             21545..22477
                     /locus_tag="ACHKUN_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO6658432.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            22495..23133
                     /locus_tag="ACHKUN_000102"
     CDS             22495..23133
                     /locus_tag="ACHKUN_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6658433.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVSALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            23442..24170
                     /gene="mpaA"
                     /locus_tag="ACHKUN_000103"
     CDS             23442..24170
                     /gene="mpaA"
                     /locus_tag="ACHKUN_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO6658434.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(24145..25110)
                     /gene="ycjG"
                     /locus_tag="ACHKUN_000104"
     CDS             complement(24145..25110)
                     /gene="ycjG"
                     /locus_tag="ACHKUN_000104"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO6658435.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            25229..25735
                     /gene="tpx"
                     /locus_tag="ACHKUN_000105"
     CDS             25229..25735
                     /gene="tpx"
                     /locus_tag="ACHKUN_000105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO6658436.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(25779..27320)
                     /gene="tyrR"
                     /locus_tag="ACHKUN_000106"
     CDS             complement(25779..27320)
                     /gene="tyrR"
                     /locus_tag="ACHKUN_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO6658437.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(27468..28529)
                     /gene="ycjF"
                     /locus_tag="ACHKUN_000107"
     CDS             complement(27468..28529)
                     /gene="ycjF"
                     /locus_tag="ACHKUN_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO6658438.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(28526..29923)
                     /gene="ycjX"
                     /locus_tag="ACHKUN_000108"
     CDS             complement(28526..29923)
                     /gene="ycjX"
                     /locus_tag="ACHKUN_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO6658439.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            30078..31076
                     /gene="ycjW"
                     /locus_tag="ACHKUN_000109"
     CDS             30078..31076
                     /gene="ycjW"
                     /locus_tag="ACHKUN_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6658440.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKAEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(31187..32092)
                     /gene="ompG"
                     /locus_tag="ACHKUN_000110"
     CDS             complement(31187..32092)
                     /gene="ompG"
                     /locus_tag="ACHKUN_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO6658441.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(32137..33219)
                     /locus_tag="ACHKUN_000111"
     CDS             complement(32137..33219)
                     /locus_tag="ACHKUN_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6658442.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(33233..33892)
                     /gene="pgmB"
                     /locus_tag="ACHKUN_000112"
     CDS             complement(33233..33892)
                     /gene="pgmB"
                     /locus_tag="ACHKUN_000112"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO6658443.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(33889..36156)
                     /gene="ycjT"
                     /locus_tag="ACHKUN_000113"
     CDS             complement(33889..36156)
                     /gene="ycjT"
                     /locus_tag="ACHKUN_000113"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO6658444.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(36153..37208)
                     /gene="ycjS"
                     /locus_tag="ACHKUN_000114"
     CDS             complement(36153..37208)
                     /gene="ycjS"
                     /locus_tag="ACHKUN_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6658445.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(37218..38006)
                     /gene="ycjR"
                     /locus_tag="ACHKUN_000115"
     CDS             complement(37218..38006)
                     /gene="ycjR"
                     /locus_tag="ACHKUN_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO6658446.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(38024..39076)
                     /gene="ycjQ"
                     /locus_tag="ACHKUN_000116"
     CDS             complement(38024..39076)
                     /gene="ycjQ"
                     /locus_tag="ACHKUN_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO6658447.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNLIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(39107..39949)
                     /gene="ycjP"
                     /locus_tag="ACHKUN_000117"
     CDS             complement(39107..39949)
                     /gene="ycjP"
                     /locus_tag="ACHKUN_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6658448.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(39936..40817)
                     /gene="ycjO"
                     /locus_tag="ACHKUN_000118"
     CDS             complement(39936..40817)
                     /gene="ycjO"
                     /locus_tag="ACHKUN_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6658449.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(40838..42130)
                     /gene="ycjN"
                     /locus_tag="ACHKUN_000119"
     CDS             complement(40838..42130)
                     /gene="ycjN"
                     /locus_tag="ACHKUN_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6658450.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(42144..43823)
                     /gene="ycjM"
                     /locus_tag="ACHKUN_000120"
                     /pseudo
     CDS             complement(42144..43823)
                     /gene="ycjM"
                     /locus_tag="ACHKUN_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(44035..44349)
                     /gene="pspE"
                     /locus_tag="ACHKUN_000121"
     CDS             complement(44035..44349)
                     /gene="pspE"
                     /locus_tag="ACHKUN_000121"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO6658451.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(44424..44645)
                     /gene="pspD"
                     /locus_tag="ACHKUN_000122"
     CDS             complement(44424..44645)
                     /gene="pspD"
                     /locus_tag="ACHKUN_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO6658452.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(44654..45013)
                     /gene="pspC"
                     /locus_tag="ACHKUN_000123"
     CDS             complement(44654..45013)
                     /gene="pspC"
                     /locus_tag="ACHKUN_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO6658453.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(45013..45237)
                     /gene="pspB"
                     /locus_tag="ACHKUN_000124"
     CDS             complement(45013..45237)
                     /gene="pspB"
                     /locus_tag="ACHKUN_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO6658454.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(45291..45959)
                     /gene="pspA"
                     /locus_tag="ACHKUN_000125"
     CDS             complement(45291..45959)
                     /gene="pspA"
                     /locus_tag="ACHKUN_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO6658455.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            46126..47103
                     /gene="pspF"
                     /locus_tag="ACHKUN_000126"
     CDS             46126..47103
                     /gene="pspF"
                     /locus_tag="ACHKUN_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO6658456.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(47223..48488)
                     /gene="puuE"
                     /locus_tag="ACHKUN_000127"
     CDS             complement(47223..48488)
                     /gene="puuE"
                     /locus_tag="ACHKUN_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO6658457.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(48526..49806)
                     /gene="puuB"
                     /locus_tag="ACHKUN_000128"
     CDS             complement(48526..49806)
                     /gene="puuB"
                     /locus_tag="ACHKUN_000128"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO6658458.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(49808..51295)
                     /gene="puuC"
                     /locus_tag="ACHKUN_000129"
     CDS             complement(49808..51295)
                     /gene="puuC"
                     /locus_tag="ACHKUN_000129"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO6658459.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(51570..52127)
                     /gene="puuR"
                     /locus_tag="ACHKUN_000130"
     CDS             complement(51570..52127)
                     /gene="puuR"
                     /locus_tag="ACHKUN_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO6658460.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(52154..52918)
                     /gene="puuD"
                     /locus_tag="ACHKUN_000131"
     CDS             complement(52154..52918)
                     /gene="puuD"
                     /locus_tag="ACHKUN_000131"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO6658461.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            53130..54548
                     /gene="puuA"
                     /locus_tag="ACHKUN_000132"
     CDS             53130..54548
                     /gene="puuA"
                     /locus_tag="ACHKUN_000132"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO6658462.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            54697..54861
                     /locus_tag="ACHKUN_000133"
     CDS             54697..54861
                     /locus_tag="ACHKUN_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658463.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            54851..56236
                     /gene="puuP"
                     /locus_tag="ACHKUN_000134"
     CDS             54851..56236
                     /gene="puuP"
                     /locus_tag="ACHKUN_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO6658464.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            56370..56615
                     /gene="ymjA"
                     /locus_tag="ACHKUN_000135"
     CDS             56370..56615
                     /gene="ymjA"
                     /locus_tag="ACHKUN_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO6658465.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            56928..58571
                     /gene="sapA"
                     /locus_tag="ACHKUN_000136"
     CDS             56928..58571
                     /gene="sapA"
                     /locus_tag="ACHKUN_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO6658466.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            58568..59533
                     /gene="sapB"
                     /locus_tag="ACHKUN_000137"
     CDS             58568..59533
                     /gene="sapB"
                     /locus_tag="ACHKUN_000137"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO6658467.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            59520..60410
                     /gene="sapC"
                     /locus_tag="ACHKUN_000138"
     CDS             59520..60410
                     /gene="sapC"
                     /locus_tag="ACHKUN_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO6658468.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            60410..61402
                     /gene="sapD"
                     /locus_tag="ACHKUN_000139"
     CDS             60410..61402
                     /gene="sapD"
                     /locus_tag="ACHKUN_000139"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO6658469.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            61404..62210
                     /gene="sapF"
                     /locus_tag="ACHKUN_000140"
     CDS             61404..62210
                     /gene="sapF"
                     /locus_tag="ACHKUN_000140"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO6658470.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            62278..62631
                     /gene="ycjD"
                     /locus_tag="ACHKUN_000141"
     CDS             62278..62631
                     /gene="ycjD"
                     /locus_tag="ACHKUN_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO6658471.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            62999..63787
                     /gene="fabI"
                     /locus_tag="ACHKUN_000142"
     CDS             62999..63787
                     /gene="fabI"
                     /locus_tag="ACHKUN_000142"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO6658472.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            63932..65059
                     /gene="yciW"
                     /locus_tag="ACHKUN_000143"
     CDS             63932..65059
                     /gene="yciW"
                     /locus_tag="ACHKUN_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO6658473.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            65127..67061
                     /gene="rnb"
                     /locus_tag="ACHKUN_000144"
     CDS             65127..67061
                     /gene="rnb"
                     /locus_tag="ACHKUN_000144"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO6658474.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(67139..67243)
                     /locus_tag="ACHKUN_000145"
     CDS             complement(67139..67243)
                     /locus_tag="ACHKUN_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658475.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            67297..69282
                     /gene="pdeR"
                     /locus_tag="ACHKUN_000146"
     CDS             67297..69282
                     /gene="pdeR"
                     /locus_tag="ACHKUN_000146"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6658476.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(69264..69392)
                     /gene="ymiD"
                     /locus_tag="ACHKUN_000147"
     CDS             complement(69264..69392)
                     /gene="ymiD"
                     /locus_tag="ACHKUN_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO6658477.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            69430..69603
                     /gene="yciZ"
                     /locus_tag="ACHKUN_000148"
     CDS             69430..69603
                     /gene="yciZ"
                     /locus_tag="ACHKUN_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO6658478.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            69693..70442
                     /gene="yciT"
                     /locus_tag="ACHKUN_000149"
     CDS             69693..70442
                     /gene="yciT"
                     /locus_tag="ACHKUN_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO6658479.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            70711..70929
                     /gene="osmB"
                     /locus_tag="ACHKUN_000150"
     CDS             70711..70929
                     /gene="osmB"
                     /locus_tag="ACHKUN_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO6658480.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT        17390 a        18304 c        17589 g        17692 t
ORIGIN      
        1 taaactaacg aaatcagaat gcgaaaaaat gctctcaggt aaaaaagaag caggcgtttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 tgcattgtat tcaaattaaa acttcatagc tgattattaa taatcaacat cgggcgtcaa
      181 tttcagtcta acattggcgc ctgccagagg tgatgcgatg gcacaagtaa tctttaatga
      241 agagtggatg gttgaatacg gcctgatgct tcgcactggt ctgggggcca gacaaattga
      301 agcataccgc cagaactgtt gggtggaggg cttccacttc aaacgagtat ctcctttagg
      361 taagccagac agcaaacgag ggattatctg gtacaactat ccaaagataa atcagtttat
      421 caaagactca tgatatgtct aaattaccaa caggtgtcga gattagaggt agatacattc
      481 gcatctggtt catgtttcga ggaaaacgat gtcgggaaac attaaaaggc tgggagatta
      541 caaacagtaa tattaaaaag gccggaaatt taagagcgct gatagttcat gaaataaact
      601 ccggtgaatt tgagtattta agacgttttc cccagtccag cactggggca aaaatggtga
      661 caacgagagt cataaaaacg ttcggagagc tttgtgatat ctggacaaaa attaaagaga
      721 cagagttaac aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca ctcagaataa
      781 taatttgtga aagtaccctg atatcacata ttcgttatag cgatatctta aactaccgga
      841 atgaactgct gcatggagaa acgctttacc tggataatcc aagatccaac aaaaaaggaa
      901 gaaccgtgcg cacagttgat aactatatcg ccctgctctg ttcgctgtta cgttttgcgt
      961 atcagtcggg atttatatca accaaaccat ttgaaggagt aaaaaaatta cagcgaaaca
     1021 gaataaagcc tgatccgtta tctaaaacag aattcaatgc attaatggaa agtgaaaaag
     1081 gacagagcca gaacatgtgg aaatttgcag tttactcagg acttcgtcac ggggaactgg
     1141 cagctctggc gtgggaggat gtggatctcg aaaagggaat agtgaatgtc agaagaaacc
     1201 tgacgatact tgatatgttc ggtcccccaa aaacaaatgc cgggatccgg acagtaacac
     1261 tactgcagcc tgctcttgaa gcactgaagg agcaatacaa actgaccggg catcatcgca
     1321 aaagcgaaat caccttttat catcgggagt acggcagaac cgaaaagcaa aaactgcatt
     1381 ttgttttcat gcccagggtg tgtaacggaa aacaaaaacc ttattactcg gtaagcagtt
     1441 tgggggcaag gtggaatgca gcagtaaaac gtgctggtat tcgccgccgt aatccgtacc
     1501 atacgcggca tacttttgcc tgctggctgt tgacggcagg agcgaacccg gcatttatag
     1561 ccagccaaat ggggcatgaa actgcgcaga tggtgtatga aatttacggt atgtggattg
     1621 atgacatgaa cgacgaacag atagccatgt tgaatgcgcg gttatcatag ttgcaaagtt
     1681 tgcccccaat ttgccccatt tagtaccaga gaactgaaat aatgcaagaa aatcaacaaa
     1741 ttacaaagaa agaacaatac aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg
     1801 gcgaagccat tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct
     1861 ccgggggtaa agacagctat accatgctgg agattctgcg caatttgcag caaagcgcgc
     1921 caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac
     1981 acgttctgcc cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaata
     2041 cttacggtat cgtgaaagag aagattccag agggcaaaac cacttgctca ctgtgttctc
     2101 gccttcgtcg cggtatcctt tatcgtaccg caacggaact gggggcgacg aagatcgcgt
     2161 tgggtcacca tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca
     2221 agatgaaagg tatgcctccg aaactgatga gcgatgatgg caaacatatc gttattcgtc
     2281 cgctggccta ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa gcgttcccga
     2341 ttattccgtg caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca
     2401 tgttgcgtga ctgggataaa cgttatccag ggcgtatcga gacgatgttc agcgcgatgc
     2461 agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt cgattttaaa ggcattaccc
     2521 acggttctga agtggttaac gggggtgatc tggcgtttga tcgcgaagag atcccactac
     2581 aaccggcggg ctggcagcca gaagaagatg aaaatcagtt ggatgagtta cgactgaatg
     2641 tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg ctactcttat
     2701 ggaggccgga taagacgcgg ccagcgtcgc atccggcaat cccgaataag atgtttactc
     2761 ttgcacccgg caattcaaca tttcattatt ttaataaccg cacccggcac gtttttcctt
     2821 taatcttccc gccctgtaac tgtttccatg ctttatgagc aacagcctga cggaccgcga
     2881 catagacatg cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat
     2941 ctcctgtcag tgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga
     3001 tacacaacgt tgccatttct gcttccagcg gcacaatgga actattagct ggcggcgttt
     3061 gccagttaag ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg
     3121 gagcacagaa actgatcgcc agaccgctat ttcctgcacg agctgtacga ccgatgcgat
     3181 gtacatgaac ttcagggtcc cacgccagct caaagttcac caccagctca agcgatttaa
     3241 tatccagacc acgcgcagca acatcagtag caaccagtac acgggcgcta ccgttagcaa
     3301 aacgtaccag agtctgatcg cgatcgcgtt gctccagatc gccgtgtaac gacaatgcac
     3361 tttgccctac ttcattcagc gcgtcgcaga cagcctggca atcttttttg gtattgcaaa
     3421 acaccacgca agaggatggc tgatgcaagc ttaataaccg ttgcaacaga gggattttgc
     3481 ctttgctgga tgtctcataa aattgttgtt caatgggtgg caaagcatct gttgagtcaa
     3541 tttcaatcgc caaaggatcg cgttgtactc gtccgctgat tgcggcgatg gcttccggcc
     3601 aggttgccga aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa
     3661 tggcatcgct aaatcccata tccagcatgc ggtcggcctc atccatcacc aaagtattca
     3721 gcgcatccag tgataccgtg cctttttgca agtgatccag caaacgaccc ggcgttgcca
     3781 cgataatatg cggcgcatgt tgcaacgaat cacgctgcat accgaacggt tgaccaccgc
     3841 acaacgtcaa aattttggta tttggcagaa aacgcgccag ccgacgcaat tcacctgcca
     3901 cctgatccgc cagttcacgc gtaggacaca gcactaaagc ctgggtttga aatagcgacg
     3961 catcaatttg ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg ccggttttcg
     4021 cctgcacgcg aacatctttt ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg
     4081 tcatggttaa ataacccaac tcattaaggt tcgtgagttg ggcgggaggc aaaacattca
     4141 gggtagaaaa agcggtcaca atctattctc gtggtcatcg acgcaaagtt agcaggcgcg
     4201 tatcctcgca gatctacgct cacgatgcga caatttaatc ggttcttcat cgggtggtgg
     4261 gtcaggcatg ggttgcgggc gagggatcgg atcgggcact ggaacaggat cgctaggaat
     4321 cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct ccggtcattg
     4381 ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc gactcatcaa
     4441 agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa gtaagacaat
     4501 atggagcgca acgcgcaacg cccatcgctt gacgttgcat tcacctgcaa tggagatatt
     4561 gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt tttgtataca
     4621 gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca taaagcaaca
     4681 ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg ccacccgccg
     4741 ccagggatcc caccaaggtt gacgccaaat aaccctgtca gaaaggtact gggtaaaaag
     4801 accattgcca tcaatgacat tgtataggta cgacgagcta aattttcctg catcacctga
     4861 gcgatttcat ccgccatcac accagtccgt gctatacagg cgtcgatttc gtcaaggccg
     4921 cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct catccacggc
     4981 aaacgttcac tggcaagacg agcatagaca tcacgttgcg gtgccatata gcgacgcatc
     5041 acgattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg ctgatcaagg
     5101 agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc gctggaatga
     5161 tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg acccgtgccc
     5221 tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg ggtcgaaaca
     5281 attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg ttcatcggtg
     5341 ctgccgttta tacagcgcaa tgtaataagc gtgccttcac cgagacggct gactcggggc
     5401 cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg tgttgtcgcc
     5461 agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg atgcgcttca
     5521 tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag catccaggca
     5581 aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt gcctccatca
     5641 tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct catcacccca
     5701 ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca aaatgctttt
     5761 ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat tatctgatca
     5821 tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata acgggatctc
     5881 aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct ggattgtttc
     5941 gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga tgcaagacct
     6001 cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag aactgccgaa
     6061 atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg ttcagcgtaa
     6121 agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag aaacagaacc
     6181 ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag ccagtcgctc
     6241 ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga ctaactctgc
     6301 accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta acctcgccgc
     6361 gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct gggaaattaa
     6421 tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac ccggcggtca
     6481 taaacctttg aattttgttc ataaactggc ggatggatct actcgtcatg tgcagaccta
     6541 tgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc atgatattac
     6601 tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg cgatgaccgg
     6661 attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc agcacctcgc
     6721 catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac acattaacga
     6781 tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca ccctcgaaag
     6841 ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg tcttattgct
     6901 accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgtg taagcgtggc
     6961 aaaagtgagt atttcgggct taccacgttt taccgtcagc attggtgtgg cgcatcacga
     7021 aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt atcgggcgaa
     7081 aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc gagatcaacg
     7141 gctgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt tacgtagtgc
     7201 gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg cctgaacatc
     7261 atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat gcgatttatc
     7321 atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca tggcttgttg
     7381 aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa gattcaggct
     7441 cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt gcaacccttc
     7501 ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg tggtgaggaa
     7561 attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg ttggatgcca
     7621 gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa gaaacagcga
     7681 tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat gataaccaga
     7741 ccgggtcggt ccaacaacgt cttacccaaa attccagtaa taagttccaa atattgccga
     7801 tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa tgtaacttat
     7861 attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac cgttataaca
     7921 cagtcaccgg cgcagaggag acaatgccgg acttaagacg cggatgcact gctgtgtgta
     7981 ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta actttgcaga
     8041 tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt ctggcgacta
     8101 tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac cggcaacatc
     8161 accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat gctgagaaaa
     8221 tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac accgacctgt
     8281 ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact gccgtgtggc
     8341 gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc aatggcgggg
     8401 tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa agtaaattcg
     8461 tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc ctgacgcaat
     8521 tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg cggatgctgc
     8581 tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc ccccatgccg
     8641 atattaatct gccctgccag ttgcccttgt cgttggcgaa tttcctcttg cgctgcgcgc
     8701 agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc agttaacgtc
     8761 acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag ctcctgaata
     8821 gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg aatgctgccc
     8881 tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa agccatgacg
     8941 ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa tgcagatgat
     9001 tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg ataagtaaaa
     9061 actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt tatggagtct
     9121 ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg tgattttcat
     9181 cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc ggaagaattg
     9241 cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag tagccggatg
     9301 ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca acagggtgcg
     9361 ctagcacaat ggattgcggt ttttgaaggt ggtttcacgg gtatcgtcgc caccctggat
     9421 accggtcgcc ccggtccggt tatggctttc cgtgtcgata tggacgcgct ggatctcagt
     9481 gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg taacgccgga
     9541 atgatgcatg cctgtggtca tgatgggcat accgccattg ggcttgggct ggcgcatacc
     9601 cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca gcctgcagag
     9661 gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga tgttgattat
     9721 tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg cggcagtgat
     9781 aattttatgg caaccaccaa atttgacgcg cacttcaccg ggaccgccgc tcacgcaggc
     9841 gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagcaac tcttgcactg
     9901 catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt tatgcaggca
     9961 ggaagcggcc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac acgcggggcc
    10021 agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca gggcgcagca
    10081 accatgtatg gtgtcggcgt tgaaactcgc ctgatgggtg cagctaccgc cagttctcct
    10141 tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg ggtcaatcag
    10201 gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat gatggcccgc
    10261 gtgcagcacc atcaagggca agcctcctac gtggtgtttg gcacacagct ggcggcaggt
    10321 catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt cgaaacgctg
    10381 gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa atctatcgtt
    10441 ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc gatcaaatct
    10501 gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg gcttcggcgc
    10561 tggaatctgc aagcttcacc gttacccgca acgtaggcaa tatcccaaat gcatttattg
    10621 cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac gccctggcag
    10681 gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt gaaaatggtc
    10741 acggttgcgg acacaatttg ctgggaaccg ccgcctttgc cgctgcaata gccgtcaaga
    10801 aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt cctggcgaag
    10861 aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat gtggatgcgg
    10921 cactcacctg gcacccggaa gcctttgccg gcatgttcaa tacccgcacg ctggcaaaca
    10981 ttcaggcatc atggtgcttt aaagggatcg cagcacatgc cgcgaattcc cctcatttgg
    11041 gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc ctcaacgaac
    11101 atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg atctcgccca
    11161 acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa atgaccgacg
    11221 tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg atgaccgaaa
    11281 ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat cgcaccttag
    11341 aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac tccgaagaac
    11401 tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa aacagtctga
    11461 ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt catcgtgaaa
    11521 cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt gcggcatcga
    11581 ctgatgtcgg cgacgtcagt tggaaactgc ctgttgccca gtgtttcagc ccctgttttg
    11641 ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca tctattgctc
    11701 ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat ctcttcattg
    11761 attcagggct attgcaagaa tgccaacaag agcatcagca agttacggac acgcaaccgt
    11821 atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa caacaacgca
    11881 aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc tcccaatccg
    11941 ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tccccatcct tttttgctct
    12001 ttatctattt gataatcgta ctcatggtga cgacggcaat tttgtcagcc tttggcgtca
    12061 gtgcgaaaaa cccgaccgat ggtacgccgg tggtggtgaa aaacctgctc agtgtggaag
    12121 gattacactg gtttttaccc aatgtgatta aaaactttag cggttttgct ccacttggag
    12181 cgatcctggc gctggtttta ggtgccggcc tagcggagcg cgtcggctta ctgccagcgc
    12241 taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg gtgctgttta
    12301 ttgctttttt cagccacatt tcttccgatg cggcgttagt gatcatgcca ccgatgggtg
    12361 cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct gccattgcag
    12421 gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg ttgctgtcgg
    12481 ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt gtaattgata
    12541 actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc ttgataaccg
    12601 acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag aaactgcaga
    12661 cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg ctacttttta
    12721 ttgctgcgat cgcgctgatg gtgatcccgg aaaacgggat attgcgcgat ccgattaatc
    12781 acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actaatcatt ctttttttct
    12841 ttgtggtttc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag gcggatttac
    12901 cgcatttaat gattgaaccg atgaaagaga tggcgggatt tatcgtgatg gtttttcccc
    12961 tcgcccagtt tgtcgccatg tttaactgga gcaacatggg gaaattcatc gccgtggggc
    13021 tgaccgatat acttgaaagt tcagggctta gcggcatccc ggcgtttgtc ggtctggcgt
    13081 tgctttcctc tttcttatgc atgtttattg ccagcggttc cgcaatctgg tcgattctgg
    13141 cccccatttt cgtaccaatg tttatgctac ttggctttca cccggcattt gcgcaaatcc
    13201 tctttcgtat tgccgactca tccgtattgc ctttagcgcc ggtatctcct tttgttccac
    13261 tgtttcttgg attcctgcaa cgctacaaac cagacgcgaa actgggtact tactattcgt
    13321 tagtcttgcc ctatccgctt atctttttgg tggtatggct gctgatgttg ctggcgtggt
    13381 atcttgttgg tctgccgata ggtccgggga tttacccacg tttgtcttaa gagagaacgg
    13441 atgctgagat tacttgaaga aaaaattgcc acgccactgg gtccactgtg ggtgatgtgc
    13501 gatgagcaat ttcgcctgcg ggcggttgaa tgggaagagt acagcgaacg catggtgcag
    13561 ctgctggaca tccattatcg taaagaaggc tatgagcgca tttctgccac caacccaggc
    13621 ggtttaagcg acaagcttcg tgattatttt gccggtaatc ttagcattat tgatacgctt
    13681 cccactgcca caggggggac gccatttcag cgcgaagtct ggaaaactct acgcactatc
    13741 ccctgcgggc aggtaatgca ttacggccaa ctggctgaac aattgggccg ccctggcgcg
    13801 gcgcgtgccg taggtgcggc aaatggatcg aatcccatca gcatcgtcgt accttgccat
    13861 cgggttattg gtcgaaacgg caccatgacc ggatatgcag gcggagttca gcgaaaagag
    13921 tggttattgc gccacgaagg ttatcttttg ctgtaaacat taaacaattt gtgccagctt
    13981 gttcacactt ttatgtaaag ttacccttaa caacttaagg gttttcaaat agatagacat
    14041 atatttacat ctaatatcgg aattctctgc tgttaaggtt tgcttagact tacttgctcc
    14101 ctaaaaagat gttaaaattg acaaatatca attacggctt gagcagacct atgatcccgg
    14161 aaaagcgaat tatacggcgc attcagtctg gcggttgtgc tatccattgc caggattgca
    14221 gcatcagcca gctttgcatc ccgttcacac tcaacgaaca tgagcttgat cagcttgata
    14281 atatcattga gcggaagaag cctattcaga aaggccagac gctgtttaag gctggtgatg
    14341 aacttaaatc gctttatgcc atccgctccg gtacgattaa aagttatacc atcactgagc
    14401 aaggcgacga gcaaatcact ggtttccatt tagctggcga cctggtggga tttgacgcca
    14461 tcggcagcgg ccatcacccg agcttcgcgc aggcgctgga aacctcgatg gtatgtgaaa
    14521 tcccgttcga aacgctggac gatttgtccg gtaaaatgcc gaatctgcgt cagcagatga
    14581 tgcgtctgat gagcggtgaa atcaaaggcg atcaggacat gatcttgctg ttgtcgaaga
    14641 aaaatgccga ggaacgtctg gctgcattca tctacaacct gtcccgtcgt tttgcccaac
    14701 gcggcttctc ccctcgtgaa ttccgcctga cgatgactcg tggcgatatc ggtaactatc
    14761 tgggcctgac ggtagaaacc atcagccgtc tgctgggtcg cttccagaaa agcggtatgc
    14821 tggcagtcaa aggtaaatac atcaccatcg aaaataacga tgcgctggcc cagcttgctg
    14881 gtcatacgcg taacgttgcc tgatttttcc gcataactca ctatccttct gtcatatcat
    14941 taaatttttc tgatttattg atctggcaga aggttcatca ctgtttcatt caccagatat
    15001 gggttaatct tttaattaca aactgcgttg acagttgttg taaggagacc ctgtatggct
    15061 atgtatcaga acatgctcgt tgttatcgat cctaaccagg acgaccaacc agcattgcgg
    15121 cgagctgttt atttacatca acggattggt ggcaaaatta aagccttttt gccgatctat
    15181 gacttctcat acgaaatgac caccctgctc tccccggacg aacgtaccgc tatgcgtcag
    15241 ggcgtcatca gccagcgtac agcctggatc cacgagcagg caaaatatta tctcaatgct
    15301 ggcgttccca ttgaaattaa agtggtctgg cataaccgtc ccttcgaagc catcattcag
    15361 gaagtgatca gcggcgggca cgatttggtg ctaaaaatgg cgcaccaaca tgaccgtctg
    15421 gaagcggtga tttttacgcc aacggactgg catctgttac gcaaatgccc aagcccggtg
    15481 tggatggtga aagaccagcc gtggccggaa ggaggcaagg cgctggtggc ggtgaatctc
    15541 gccagtgaag agccgtatca taatgcgctc aatgaaaaac tggtcaaaga gacgatcgaa
    15601 ctggcagaac aagtcaacca taccgaagtt catctggttg gcgcttatcc ggtaacgcca
    15661 attaatatcg cgattgaact gccggaattt gatccgagcg tttataacga tgccattcgt
    15721 gggcaacatt tgctggcaat gaaagctctg cggcagaaat tcggcattaa tgaaaacatg
    15781 acgcacgtag aaaaaggtct gccagaagag gtgattcctg atttggcgga gcacttacag
    15841 gcggggattg tggttctggg cacggtagga cgcaccggta tttcagcagc attcctcggc
    15901 aacacggcgg aacaggtgat tgatcatctt cgctgcgatc tgttggtcat taaacctgac
    15961 cagtatcaga cacccgttga actggatgac gaagaagacg attaacgact cttcaataac
    16021 tgacaaggcc cgagttatcg ggcctatttt ttacttactg ctcttcgccg ccaaggaaat
    16081 aaattcccaa cgggattgcc agcaacacag taaagaccag gctataaaca aaaatcatcg
    16141 atgactgaat cacatacatt gatgtcgtcc actcagacag cggaatattg tactgttcta
    16201 taacgccgcc gatagtcgcg cgagtcacaa ccgacgccgc aataatgaaa accgccaaca
    16261 aaccaaagag aaatttcttc cctttcgctg acttcagttt cgccataatc atgatgtaat
    16321 ccttgtgcag acaaatttct tgttacctaa acgtaacaat accatgacat ttcaccgttg
    16381 tctgctctga aaacctcacc tcttagctgg atttacaatc aacgcattga ttagttagct
    16441 aatgttgcta cttccttccc tgactattga tgatctgaat gaattgtcgc ggtaaaatag
    16501 ccccgccagg ggtttccggc atgacgacaa ttatctggag gagcctgata aaacaatgat
    16561 cgctgaactg tttacaaata atgcgcttaa tctggtcatt attttcggta gctgcgcagc
    16621 attgattctg atgagctttt ggtttcgccg cggaaatcgt aaaagaaaag gttttttatt
    16681 ccatgcggtg caatttttaa tctacaccat aattatcagt gctgttggta gcattattaa
    16741 ttatgttata gaaaactata aactcaaatt tatcacccca ggcgttatcg attttatctg
    16801 tacgtccctg attgcggtta ttctgacgat taagctattt ttgctgatta atcagtttga
    16861 aaaacagcag attaaaaaag gtcgcgatat caccagtgcg cggattatgt cgcgcattat
    16921 caaaatcacc attattgtgg tgcttgttct gctttatggc gaacatttcg gcatgagcct
    16981 ttctggcttg ctgacctttg gtggtattgg tggtctggct gtcggtatgg ccggtaaaga
    17041 tattctgagt aatttctttt ccgggatcat gctctatttc gaccgtcctt tcagtattgg
    17101 cgactggatc cgttcaccgg acagaaatat cgaaggtaca gtagcggaaa ttggctggcg
    17161 aattaccaaa attacgacct ttgataatcg tccattgtac gtaccgaact cgctgttttc
    17221 gtcgatcagc gtagaaaacc caggacgaat gaccaaccgc cgcattacca cgaccattgg
    17281 tttacgttat gaggatgcgg caaaagtggg cgttattgtc gaagccgtac gtgagatgct
    17341 gaaaaatcac ccggccatcg accagcgaca aaccttactg gtttatttca accagtttgc
    17401 cgactcgtca ttgaatatta tggtttattg ctttaccaaa accacggtat gggctgaatg
    17461 gcttgctgca cagcaagacg tttatttgaa gattatcgat attgtgcagt cacacggcgc
    17521 ggactttgcc ttccccagcc agacgctgta tatggataac attacaccgc cggaacaggg
    17581 gcgctaaatc agacatcact ggcgttatcc acaagggtaa cgccagttcc catcacatca
    17641 atgcttcaca atatacatag tccggctgta cgcaacatct tccgggttat taatgggata
    17701 acctttcagc cacggcttga ttaatcgtcc attggtatat tgataaattg gtgcaatcgg
    17761 tgcttgctcc atgaggattt tttctgccgc gttgtaatcg gcattacgcg ctttaacggt
    17821 attttccatc gatgcctggg ccagaacttt gtcgtatgcc ggattgttaa agcgtgaaat
    17881 atttcctgaa tgcgttgacg ttaataatgt caggaaagtg gagggttcat tataatcccc
    17941 cacccatgag gcgcggataa catcaaaatt gccggtgtta cggctatcga tataggtttt
    18001 ccattcctga ttttgcaatt taacatctac gccaaggttc tttttccaca tcgatgctac
    18061 agcaatcgca attttttgat ggttttctga agtgttatac aaaagcgtca gcttcagcgg
    18121 tttttgcgga ccataaccag ctgcgctcaa caaagttttt gcctgcgcat tcagttcttc
    18181 ctgactcatt tgttcaaacg gcgaaggttc cggcgtaaat ccggcggtaa catctggtgt
    18241 aaaatgccac gctggctttt cgcccgttcc caacactttt tcggtcatca ggcggcgatc
    18301 tatcgtcata cttaatgcca ggcgaacgcg ctgatctgcc gtcggccctt tttgcgtgtt
    18361 aaacgcataa taataggtcc cgagctgtgg cggcgtataa acctgccccg gaatatcctt
    18421 caacagcttc tgatacatat ttttcgggaa ggattcggtg atatcaatat cccccgcgag
    18481 gtaacgctta gtggctgcgg attcctgatt aattggcagg aaggtcactt tttgcagtac
    18541 cgttttggcg ttatcccaat aatgggtatt cggtaccacg accagttttt cattgactac
    18601 gcgatcttta agaacataag cgccattgcc gatcagattt ccgggtttcg tccactcttt
    18661 accgctttct acgttggctt tttgcaccgg gaagaaggca aagttagcgg ttaaattcac
    18721 aaaccacggc aacggtttat caagctgaat tttcaaagta tgggcatcaa ctgcggtgac
    18781 gccaagctgg tctggcgtag ctttaccatc aataatcgcc tgtgcgttgt tgattcccgc
    18841 cagcgcggca aaccatgcaa atggcgatag cgttttcggg tccaccagac gttgccagct
    18901 gtagacaaaa tcttgcgccg ttaccggtgt gccatccgcc cattttgcgt tatcgcgcag
    18961 ggtaaaagtc cagatacggt tgtcattact tttccactga gtcgcaacgc cggggacaat
    19021 ctcccctttc tcgttctgat tcaccagtcc ttcaaacaga tcgcgaatga cctgaatctc
    19081 tggcaggccc acggctttag cgggatccag cgacgcaggc tcatctttaa tgtggcgcac
    19141 cagctcctgc ttctctgcca gtactgtgcc gctcggaact tctgcagcat acgaaagaga
    19201 aatgctgctg accaacagcg cacaacacgt gactgaaaca gagtgcttca taagtttccc
    19261 tctaaccgac attaaaattg atgcgtaatt atttgtttcg ccttaaagaa atgcaaacaa
    19321 cttccatcaa aaaagtgaca caggaagcat aaaatgtttt atgtatcaat atattaaatc
    19381 agatttagtg taaataatcg acgccaaaat gcggtaaagc acatagaaca ccgaacacta
    19441 cgttcaggct tcgcacgttc aaaccagttt tcggtatcat aatcagcatc tgtcgccatt
    19501 gtacgaagaa taaaaaataa ccaaaacgtt gaaaggaact gagaatacgt taagagagaa
    19561 attaaattaa tgtatcagca agtagtgcta aacagcacct ttatacttca gcctatctat
    19621 aaataatgag aatgccgagc cagcatgtcg acggtgtgga tagtacaggt gatgaccggg
    19681 taaatctggt gtgaatttat ccaaaacacg tatcagtttt ttttctttaa tagcccgctc
    19741 aacctgatca taaggtagat acgccaatcc atgcccatca attgcagcat caatgatcag
    19801 gtctatcgta ttcagtaaaa gctgaccttc catgcgaaca cgaacttcac gtccaccgcg
    19861 tattaatctc cagcgatttg ctgtacccga tgtgggaaga tacaaattaa ttgcctgatg
    19921 atctattaat tgtgacactg acgttggaac acttcggcga gaaaaataat ccggtgagcc
    19981 aacaatagcc attggtatat ctggcccgat tcgaatggcg atcatatctt tatccatctc
    20041 cccacccaga cggacgcctg catcaaaacg ttcagaaacg acatcggtca aaccataatc
    20101 aatggtgagc tgaatatcaa tttcaggatg cgatttcagg aatgtgcgca ttgctggtaa
    20161 caatatcgtt tttgctgcat gttctacagt agtaatacgt attgtcccgg atgggcggtt
    20221 ctgcagctcg ctcagggatg ccagggctga atctatgtca tgcaacatag ggccaagaac
    20281 agacaaaaga tgctcgcccg cttcagttgg aacaacgctg cgcgtggttc gcgtcagaag
    20341 ccgcaatccc aatcgttctt ctatacgacg cactatctgg cttaaagctg actgcgccat
    20401 gctcaggcgg gctgctgcac gagtgaagct acgctcctct gcaacgacga caaacgccat
    20461 cagatcagcg atttcttcac gcttcattca gtatctccat gcaatctatt aatcatttaa
    20521 atgataggtc taatcatgat tttgggtcta atcaacttaa tgatcaatat ctatagtgat
    20581 atcaccaacc gttaaaacga accgacaact ccatgaggaa cggaatgatg aataataaag
    20641 tcagcttcac taacagcaat aatccaacca tctctttgtc tgcagtgagc tatttccctc
    20701 caaaatttga tgaaacccgt cagtatcagg caattgtggt atctcaccca ggtgggggcg
    20761 ttaaagaaca aacggccgga acctatgcca aaaaactggc ggaaaaggga tttgtcacta
    20821 ttgcttatga cgcatcttat caaggtgaaa gtggcggcga gccgcgtcag ttagaaaacc
    20881 catacattcg tactgaagac atcagtgccg tgattgatta cctgacaacg ctttcttatg
    20941 tcgacaatac ccgaattggt gcgatgggaa tttgtgccgg tgcaggatat acagccaatg
    21001 ctgcaattca ggatcgccgc atcaaggcca tcggtaccgt tagtgcggtc aatattggtt
    21061 cgatattccg taatggttgg gaaaacaatg tgaaatctat tgatgccctg ccatacgttg
    21121 aagcgggttc aaacgccaga accagcgata ttagcagcgg agaatacgcc attatgccac
    21181 tggcaccaat gaaggagtcc gatgcgccga acgaagaact gcgtcaggcc tgggagtatt
    21241 accacacccc tcgcgcgcag tatccaacag caccgggtta cgctactttg cgcagcctta
    21301 accagattat tacctatgat gcttaccata tggcggaagt gtacctgact cagccgacgc
    21361 agattgtggc gggcagccag gcagggagca aatggatgag tgacgacctg tacgatcgag
    21421 catcaagcca ggataaacgc tatcacattg ttgaaggcgc aaaccatatg gatctttatg
    21481 acggcaaagc ctatgtggct gaagcaattt cagtattagc tccgttcttt gaggaaactc
    21541 tgtaatgaaa catattcaaa tccgcaatag tgatatggcc tggcatattg ctgccaatat
    21601 tcagtttcca ccaaattttg atgagtcaaa acagtatccg gcgattatca gcgtccatcc
    21661 ttttggcagt tgtaaggagc agacgtctgg caatatctat ggcaaagcat tggctgaaaa
    21721 aggctatctc gttctcgctt atgacgccag tttccaggga gaatctggtg gggagcctcg
    21781 ctggatagaa gatccgacac agcgcgtaga ggatattagc cgtgttatcg attatgcagt
    21841 aactctgcca tatgttaatg ctgaacgtat tggcgtgctg ggagtgtgtg ggggtggtgg
    21901 ctatgccatt aatgccgcgc tgacagaaaa acgaattaaa gcagtagtga gtattacggg
    21961 tgttaatatc gggcgtcttt ttcgggaagg tttcagcaat tacgatccga ttggcgcatt
    22021 gaatgcaatg gcgtcgcaac gaaccaaaga ggctcgtggt ggagaattac agatcaacga
    22081 attacttccc gccagcctgg atgcagcaaa agcccatgga ctgaccgaac gcgatgttta
    22141 tgaagcaact gactactata aaacaccacg cgggcaacaa ccgggaggtg cgacaaaaat
    22201 gttgttctca catgcacaga aaacgctggc ctgggatgcc ttcgcattta ctgaagtgtt
    22261 actgactcag ccggtgatgg tagtagttgg tgaaaaagtc ggtgccttcg gtgcttatcg
    22321 tgatggactg gaagtctatg gccgggcgat ggtttcgcag gatcgtcagt tagtttcatt
    22381 acccgatttc tcacattatg aactgtatga caaaccggaa gcagttcaga aggcgctgga
    22441 aaaagttatt ccttttttca acacccatct gggataagtt tcatgagaaa tattatgaaa
    22501 aatgtactga ttcttggtgc tggcggtcag atagcccgcc atgtgattaa tcaactggca
    22561 gataaacaaa ccattaaaca aactttattt gcccgtcagc cagcaaaaat ccacaaaccc
    22621 tatccaacta atagcaagat tattatggga gatgtactga atcatgcagc actgaagcag
    22681 gccatgcagg gacaggacgt tgtgtatgcc aaccttacgg gggaagattt agatattcag
    22741 gcaaattcag tgatcgctgc gatgaaagcc tgtgatgtta aacgtctgat ctttgttctt
    22801 tccctcggta tttatgatga agttccagga aaatttggcg aatggaataa tgcagttatt
    22861 ggtgagcccc taaaaccatt ccgcagggct gcggatgcaa ttgaggcttc tggactggaa
    22921 tatacaattc ttcgccctgc ctggctgact gatgaagata ttattgatta tgagcttacc
    22981 tcacgcaatg aaccattcaa aggtacgatc gtatcacgta agagtgtctc agcattaatc
    23041 acagatatca tcgataaacc tgagaaacac attggtgaga atataggtat caatcaaccc
    23101 gtgactgatg gtgacaagcc attattcatg taaatcaatt acatagttac catcgtaatg
    23161 tactatgtgc agtacgattt attaaactta ttaaggtgaa ctgatcaatc cgatgcgatt
    23221 aacatgtgta gaaccagtaa gaataaaata cgccatgtat ataacattac gacttacatc
    23281 taagtcagca taattctaat atctgcacat caataaaaat gcaaacaact tccggcaaaa
    23341 aagtgataca agatcgatga catagcgttg aagaaaagga tttgataatc cgcaaaattt
    23401 gcacaacact gcctgtaata cttcctgaac cagagacgat catgaccgta aaccgcccac
    23461 gcgccgaacg cggcgcattt ccgcccggaa cagaacatta cggacgttca ttattgggcg
    23521 cgccgttaat ctggtttccg gccactgcag ccagtcgcga aagtggtttg attctggctg
    23581 gcactcacgg tgatgaaaac tcttcggtcg tcacgctctc ctgtgctctg cggactttga
    23641 caccttcttt gcgccgccat catgtagtgc tgtgtgtgaa tcctgacggc tgccagctgg
    23701 ggttacgggc caatgctaat ggtgtggatt taaaccgaaa ctttccggcg gcgaactgga
    23761 aggaaggtga aacagtttat cgctggaaca gcgccgctga agaacgtgat gtcgtattgc
    23821 tgacaggtga caaacctggc tctgaacctg aaacccaggc gctgtgccag cttatacacc
    23881 gcattcaacc cgcctgggtg gtctcattcc acgatccgct ggcctgtatt gaagatccca
    23941 gacacagcga attaggcgaa tggctggccc aggcgtttga attgcctctg gtaaccagcg
    24001 tcggttatga aacacctggc tctttcggta gttggtgtgc ggatctcaac ctgcattgca
    24061 tcaccgcaga gtttccgccc atctcctccg acgaagccag cgaaaaatat ctctttgcga
    24121 tggcaaactt gctgcgctgg catcctaaag atgcaattcg cccgtcgtga actgaagcgc
    24181 cggttccaca tctaccgcca gccaggtcgg tccgtcaaga tcgacgaaac tgacctgcgg
    24241 caccagcggt aaagcggcgc taatggcccg agaggtacac aacatgcagc ccagcatcag
    24301 acgaaaacct tgtgcacgcg cttcagtcgc cagcgccagc gcttccgtca gaccaccggt
    24361 tttatcaagc ttaatgttaa ccatctcata gcgccctttc agcgccttca gattgctacg
    24421 tgtatgacaa ctttcatctg cacaaatcgg taacggatga ataaaattct ccagcgccgc
    24481 atcgtcctgc gcaggaagcg gttgttcaag catcgcaacg cctaaatccg ccaatagctg
    24541 gcaacgcgcc gccaaccctt ctgcacgcca ggattcattt gcgtcaacga tcagcgtcgc
    24601 atcgggcaca gctgtgcgaa ttgccaccat ccgctcactg ataagatggt tatccagctt
    24661 cactttcagt aatttcgcgc ctgcctgcca gagtgttgat gcactattgg ccatctgatc
    24721 aggcgtaccg atgactaccg tctgtgcagt ggtgactgtc ccgggaagcg ttatgccgat
    24781 caaatcagcc agcgattgct gctgttttcg cgcggccaga tcccacaatg cacaatccag
    24841 cgcattacgt gctgcgccgg cagggagaat tttttgcaac tcctcccgtg tcagcccttt
    24901 ctctaattgc ggcacgacgc tcataatttg cgccattacc gaggcatcac tttccccata
    24961 acgcggatac ggcgtgcatt cgccggtgcc tttaataccc tcttcttcca gttcaaccac
    25021 caccacgcgc gcttcactgc gacttccccg ggcaatcaca aacggggtat gtaagggcca
    25081 ggcttcctca aataccttaa cggttctcat ctttactcct tgttgtcgcg gtggtctgcg
    25141 gattaagcgc aaatagcgtt tgctgtgtta ttgacagtta gcataaacta ggtgtgacgt
    25201 taactatatg taaacaggaa agataatcat gtcacaaacc gttcatttcc agggcaaccc
    25261 ggttacagtc gccaattcca tcccgcaggc gggtagcaaa gcgcagactt ttactctcgt
    25321 ggcaaaagat ctgtctgacg tcaccctcgg tcagtttgcg ggtaaacgca aagtgctgaa
    25381 cattttcccg agtattgata ccggtgtttg cgccgcatca gtacgtaagt ttaaccaact
    25441 ggcaactgag atcgacaaca ccgttgtgct gtgtatctct gccgatctgc cgttcgccca
    25501 gtctcgtttc tgcggcgcag aaggtctgaa caacgttatc accctctcca ctttccgtaa
    25561 cgctgaattt ctgcaagcct tcggtgtggc aattgctgat ggcccactga aaggtctggc
    25621 agcgcgtgcc gttgtggtta ttgacgaaaa tgacaatgtg attttcagcc agctggtgga
    25681 tgaaatcacc accgagccgg attacgaagc agctctggct gtactgaaag cataagttaa
    25741 tatgcctgat ggtgttgcac catcaggcat attcgcgctt actcttcgtt cttcttctga
    25801 ctcagaccat attcccgcaa cttattggca atcgcggtat gtgaaacgcc gagacgtttt
    25861 gccagtttgc gcgtgctggg ataattgcga taaagctggg ttaataccga gcgttcaaaa
    25921 cggctggtga tttcgtccag cgaaccttcc atcgcatctt cgcccacggc taccgttgcg
    25981 gcgtcataat ccggcaacaa aatatcctgt ggacgcagct cataaccgtc cagttgtgtc
    26041 agtgcgcgat agatagcgtt ctttaactgc cgcacatttc ccggccacgc ataacgcgta
    26101 agtacagtat tcaggtcagc ggccagtttc ggacgcggca cgccctgctc gtcggcaaag
    26161 cgggcgacga acagctcagt taacggcatg atgtcctgcg gacagtcacg tagcggcggc
    26221 agattgagcg tcaacacgtt cagacgataa tagagatctt cacggaacat gcctttttgc
    26281 accagttcga ccagattctt ctgcgtagcg caaatcaccc gcacatcgac atgcacctca
    26341 tggtcttcgc caacccgacg gaaagtgcca tcattaagga aacgcagtaa tttcgcctgc
    26401 atccgtggtg acatttcccc tatttcatcc aacagcaccg aaccaccgtt cgcctgctca
    26461 aagaatcctt tcttcccttc cggagcatga ccaaacagtt cactctcgac cgcatcttcc
    26521 ggtatagacg cacagttcag cgccaggtaa ggtttgcccg ctctggggct tgcctgatgg
    26581 caggcgtagg caaagagatc tttacctgta cctgtgtcac ccgtaatcag cagcggcgcg
    26641 cttagcatcg ccagtttctg cgcctgttcg acaacatgct tcattttcgg gctgacggcg
    26701 acaatttgac tgaaggcgct gacgtcctgg gcggcgacat tttgcaactg gcggcccata
    26761 cgaatcgttg atcgcaacat caccaccgca ccggtcagga cgtgttgatc attttcatcc
    26821 tgaagataaa caggcgtaat ctccatcagg aaattctgcc cattaataac gacatgctcg
    26881 ttatgggaat cttgcggttc gctttccagc caacgtaaaa aattaaagcc gttaatcaat
    26941 tgtgcggcgg tatggttgcg caggcgatcc aatttttgcg caaaaagctg acagctcgcc
    27001 gggttcgcca tatccacttt gcttttcata tcgacagaga gcacaggttc gggcaacgcc
    27061 tccagtaacg cgctcaacgc cagatgctca cgttcggaag gcatccacgg gacagtacgc
    27121 acatcggtaa cacctgcaat acggcgtatt tcggccatca gactgctgaa actctcaaac
    27181 tccagttcag caaaattgag gtagattcgc ccaatgggat caatctcaat accgcgtaaa
    27241 tcaatgcctc ttagcacgag tagatcgagt aattcgcggg tcagaccgag tcggtcttca
    27301 caaaagactt ccagacgcat gggaaccttc acctgaaaaa agaacaatta atatgatgat
    27361 atgacactat ttggatagca ggaaggtttc tgtcaacaat cattgacaca aagacggaaa
    27421 aacgggcact tcggcgtaaa gatatccggt cagcacccga tattgcatta tttttcgctg
    27481 ggcgttttgc ctttttgcag cgtttctttc acctgaccga taagctgacg acggaaatcc
    27541 ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc ggcaaagctc catagcttta
    27601 atcccgagtc gtgccgtcag aagtccagca ccaattccct gagctgcgcg ggtagacaaa
    27661 cgagcagcga gatcttgcga catccagtcc atccccactt cgcgcaccag ttcgctggct
    27721 ccggcaaaag cgatattcag caataccagc ttaaacagac gcaaacggct gtaatacccc
    27781 agttcaatgc catacagcgt ggcgatgcga ttaattaaac gcagattgcg ccaggcgata
    27841 aacgccatat cgaccaacgc cagcgggctg accgcaatca tcaacgttga ttccgccgcc
    27901 gaacggctga tttcgcgccg cgcctgggca tctaaaactg gctggaccaa atgcgcatac
    27961 aaactgacca cttcacggtc gttttgcgtt tcatggattg aggcatacca gcgttgcagc
    28021 gctggatgag actgatcaat acccgcctgc tgcgccagtt tttcgcaaaa tgcgcggcct
    28081 ttgcccgtgc cgtggctgtg caacaaatcg cgcgcttcgt cgcgttcatg ggcgcgctgt
    28141 cgcaagcgcc ataagcgccg ccactctgtt accacagaac ctacgccagc gccgataatc
    28201 aatgccccag cggcacatcc acccagcgcc acccagtcct gagtttgcca ggcattcatt
    28261 gtccactgta caccctgccc gacaacgctt gcgccaaaca gagccagccc gcccatcacc
    28321 attttgcgcc acaggctgcg tttcggacgt aacgctgcat ccattaccgc ttcaacttgc
    28381 ccctcttctt caggcgcttc atcgagcgtg gccggggcaa aattttgcgc ctgattttcg
    28441 tcaaaggtct gctgcgccct gaattttgga ttctgatcga cctccagcgg accgtcgaaa
    28501 tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc tcctattaaa aattcaagcg
    28561 cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc catcacctgc gggcgaaacg
    28621 cttcaaactg gaacccttgc ttgtcccaga acgcctgacc gggcaaacgc gcaggaactt
    28681 caccgggata aacggtgagt ggcgcaccat cgctgagccg gttaccgcgc aatgccggaa
    28741 ttttctcgcc gttgacgtca ataatgccgc tggtggtcgc ctgcaccgag gccagcccca
    28801 ggcaatccat actaatccct tcaaacgccg cattttgcca ggcatcctga atcagttgtt
    28861 gcagtaatga aaccatattg gcgtgctgat cgatggtcac atggtccgct ttagtggcag
    28921 caaacaatag cttatcgata accggcgaaa acaaacgccg gaacaaggta cgctgcccgt
    28981 agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat atcattaaat gcctgtggcc
    29041 cactgttgag aggttgcagg caatcaacca gcacaatctg gcggtcaaag cgcagaaaat
    29101 gattcttata gaaccccttc accaccttct cgcagtaata attaaaccgc tcgcgcagca
    29161 ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga ctcgccccag gcatcgacat
    29221 ccggccacgg gaagaattgc agcgcgggcg cacctgccat atctcccggc aagacaaagc
    29281 gcccaggctg aataaagtgc agcccctgct gtttacagtg gtggagataa tcggtccacg
    29341 cggcggcaat gtctgccagc cggttttcgt cggcaggcgc tagcgggtcc agcccttcgc
    29401 acatcattcg ccatttgacc gaccattcgc cgcgctgacc attgagtaag cccgtcatct
    29461 ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag gtcgagcaac cattcgccgg
    29521 gataatccac aatctccaga tacagcgtgg aggtgtcctt gaagtggcgt agcagcgaat
    29581 cgttcgattt aaaacgtagc gccaggcgga tttcactgac gccgcgcgtt ggcgtcggcc
    29641 aggcgggagg atcgccatat aactgcgcca gtccttcatc atatgtgaag cgcggaatgc
    29701 caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg ctcttcacgc accgcgctta
    29761 acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg attgaccatc gcagtgataa
    29821 acgctgtttt gccgctgcgg cttagtccgg ttacggcgag gcgcagatgt ctgtcgacac
    29881 cccgattcac cagcgcatta agttcatttt taagtcgctt cattgctgtc ctgtgctgct
    29941 ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt gggggccagg ccattgatta
    30001 tcaactgccc gtgttgtcat cgacgggcga actccataag atgtgcagca ttcacgttcg
    30061 gacggagttc ctgacctatg tcccctacta tttatgatat tgccagggtt gcaggcgtat
    30121 caaaatccac cgtatcacgc gtgctgaata agcaaactaa tatctccccg gaagcgcgcg
    30181 aaaaagtgtt acgggccatt gaagaattac agtatcagcc aaacaagctg gcccgcgcgc
    30241 tgacctcttc gggttttgat gccattatgg tgatttctac ccgttcgacc aaaactacgg
    30301 cgggtaatcc gtttttctct gaagttttgc atgccatcac cgccaaagct gaagaggaag
    30361 gtttcgacgt gatattgcag acgtcgcaca atccggcaga agacttacaa aaatgtgaaa
    30421 gcaaaattaa gcagaaaatg attaaaggca ttattatgct aagttcgcca gcggatgagt
    30481 cattttttgc ccaacttgat aaatacgata ttcctgtcgt ggtgattggc aaagctgaag
    30541 gtcaatatgc ccatgtttat tctgtcgata ccgataattt tggcgacagc attgcgttga
    30601 ccgatgcatt aattgaaagt gggcatcaaa atattgcctg cctgcatgct ccgcttgatg
    30661 tccatgtttc agtggatcgg gtaaatggtt ataagcagag cctggctgcg cataatattg
    30721 cagtgcgtga tgaatggatt gttgacggcg gttataccca tgaaacagca ctgcaagccg
    30781 cgcgggaatt attaagccag tcaccgttgc ctgaggcagt gtttgctact gacagcctga
    30841 aattaatgag tatttatcgt gcggcagcag agaaaaatat tgctattccg cagcagttag
    30901 cggtggtggg ttatagtaat gaaacgctgt cgtttatttt aacgcctgcg ccaggcggta
    30961 ttgatatccc aacacaggag ttaggtcaac aaagctgtga gttattattc cgcttaattt
    31021 ctggaaaacc gtcaccacag aatattaccg ttgccacgca tatgacgctg aaataaatac
    31081 cagctacctt gcctgaatgc cggatgcgat gctagcgcat cttatccggc ctacatttat
    31141 agttcggata aagtactcgt gccgcatccg gcaatttagc ccattatcag aacgagtaat
    31201 ttacgccgac acctgcataa tggaatttat cgctgtcgcc ttcgtcgtga tcctgccact
    31261 caaacgcgta ttccagcgaa acggaaagcc cgttctggaa atcataaccg taaaataaac
    31321 ctacacggtt aaaatcatgg ccttcacgtt caatatcatc ctgccagtcc cagttactcc
    31381 agcgatccag cccaatgcgc gtatacggcg tcaccgagtg gttgccgagc gtcagcggca
    31441 aataggcgcg aatttcttgc gtagaaaact caccgttatt gcggctgtcg tccatattaa
    31501 agccgcgctc gagataatag ttcactcgca aggcaaccgt ttcgttgaag gtatattgca
    31561 ggcctgtttc cgtttcgact cgggtatcag cgtaaccggt cgtgttcaga tcgttggcaa
    31621 atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc agtcagtttc acatcccagt
    31681 ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt acccggttca tcaacgtagt
    31741 gataaccata attacggaaa ccgccggtca ggccgaaact gaaatcatcg ttttcgagga
    31801 actgataatg cacctccagc tccgggcgat caaaccacgt tccacgttta cccgcgctat
    31861 aatctaccgg cccttcctga taataggcca gagcaattct ccacggcccg ttggcggcat
    31921 taaaatagac tgaaggctcc gccagcccat ccatatcttc gccataaccc tcgacgtttt
    31981 ctatttcgta catcgcgccg atattaaagt gccagtcgtt cctttcctcg gcctgtgcgc
    32041 aggccattcc cgcacacatc accagtgcgg tacagggtaa taactttttc attattgttc
    32101 cttgtattat tctgggggaa tgccttcccc ctgtatttag cgaattgcta tttccgtttc
    32161 tgcatcaaag aaatgacatt tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg
    32221 ataatcattt aacgcaccag cacggaccac taactcgtgc cccccaaccg tggtgtagag
    32281 cataaattcc gcaccggtta attctgccac gctaattttg gcggaaatgt tattttcctc
    32341 ttgcgcgtcc ggatgaatat cttccggacg tattcccatc acgatgggct tatgcaaact
    32401 tccctgtgtt ttcagaaccg ctaatttctc ttcgggaatg gttaatttaa gcgtttccgt
    32461 aacgaattta tcgccatcga tcgtgccacg aataaaattc atcgccggtg atccaataaa
    32521 tccggcaaca aacatattcg ctggttggtt ataaaccgtt ttcggcgcac caacttgctg
    32581 aacaatccca tccttcataa tcacaatccg cgtcgccatg gtcatcgctt cggtctgatc
    32641 gtgggtcacg tagatcatgg tggtattcag cttctggtgt agcttgctga tctctgcgcg
    32701 catttgcacg cgcagcttgg catcaaggtt agagagcggt tcatccatta aaaacacgcc
    32761 cgcttcgcgc acaatcgccc gcccgagcgc cacacgctgg cgttgcccgc cggaaagcgc
    32821 gcccggcttg cgtttcaggt actcacgcag gccgagaatt tgcgccgccc agttcacccg
    32881 ctcatcaatc acctctttgg cgattttttg catcttcagg ccaaacgcca tgttgtcgta
    32941 aaccgtcata tgcggataca acgcgtagtt ctggaacacc atcgctatat tgcgtgcttt
    33001 ggctggaacg tcattcattc gtttgccgtc gatcaacaga tcgccgccgc tgatctcctc
    33061 aagcccggca atcatgcgca gggtggtcga cttaccgcag cccgacgggc cgacaaacac
    33121 gatgaactct ttatcggcaa tttccaggtt gaagtccttc accacatgca cctggttatc
    33181 gtagattttt tgaatatgtt gtaacgaaag ctgagccatg ttgattcctt tgctatacgt
    33241 tttgccagaa ggccgataac cgcgaccagg tcagagagtc cgttgaaggc aacagtaatt
    33301 gcgccccggt taagcccgcg ccgatcccca ccgagcgcat accgctggcg ttaatggcgt
    33361 caatgcccgc ctgcgcatct tcaatgccga tacatgcctg cggcggcacg cccagcccgg
    33421 cacaggcggc gagaaagatt tccgggtccg gtttcgagtt tttaagttgg gaagcatccg
    33481 cgcagaaggt gaaaaactcg cgcagctcca gcgccgctaa aatcgtcggc gcattcaggg
    33541 agacagaagc cagcccaacc gggatctgct gtgcacggag atctgccagc aaagagcgaa
    33601 tgccgggtag aacagcgctg accgtcaact cgcgcagtga gtggacatag agcagatttt
    33661 tgcgatacgc cagttgcgcc ctctcctgcg agttaaagtc gccctctttg cccccgtgtt
    33721 gcagaatgcg ccgcagagac tcatcgcggc tgatcccttt tagggattcg ttaaactgcg
    33781 catcaatgct gatgccaatt tcagcggcaa tctgctgcca cgcctggaaa tgcagatgcg
    33841 cggtatcggt gattacacca tccagatcga aaattacccc ttgcagtttc attcatcctc
    33901 ctgatgcgtg gtagcggtcc cattgaaggg caaaataaaa tcacccaaac agaaaacaga
    33961 ttcttcagcc acggatataa gctgaccgtt caaacgcagt gaaacgggcg cagaagttcg
    34021 aatcgcaata cgctgcgcgt caagagtgac ctgtaattcg cagccctgcc agaacagagg
    34081 gaaagacaac tgttgccact gctcaggtaa cgccggattg agatgcaatt caccgtcacg
    34141 cacgctcacc ccggcaaaac cctgaatcgc ccccagccag atagcgccag ttgcggcagc
    34201 atggatacca tcatcacaac tatgcggatc agcaccaaga tcgatttcag tcccctcgcg
    34261 ccagaactga taactttggg tcagcaggcc acagcgtgcg gcaacaatgc cgtggattgc
    34321 tttacttaat gacgagtcgt gaatagtgcg cggttcataa aattgcagat tggcaagaca
    34381 cgatgccgct gagaactgct ccggcagcat gtaattgagc atcaccacat cagcttgttt
    34441 gaggatttgc atctcgttca cttctgcgcg tgaataatcc agcagaatgg tttgcttccc
    34501 cgccgccgct ttgtatttcg ccagattaat cgccggctta gccataaacg aatcatcctg
    34561 cggcaaaacg ccgtcgggct gaatttctgg cagccgcagt tctttaagga acatttcggc
    34621 gcgatggata aacgcatcgt cgctacagcc aaactggcgg gcaatactca gcgcctgctg
    34681 gacgttgtaa tacgccatat agctggtgaa ggcgttatta ttgacatgtt cggtatattc
    34741 gtcaggccca ataacatcat gaatttccag acggtcgtta accctcaccg cgcggctaat
    34801 ccagaacttt gcagtttcca gaagtagcgc catgccttca tgcgcaatga aactttcatc
    34861 ccccgtggtc tgccagtatt gaataaccgc ccaggcgata tcggccacca gatgatgttc
    34921 cgcctgcgcc gaggccactt tttgccgcag cccggtgcga atgttaatgg cggcaaattc
    34981 cggcgtctct tcttcgccgc tgcgcgcgct ttcccacgga aatagcgcgc cctgccagcc
    35041 gttgcgtcgc gctttctcct gcgcgcctgg caagttgtgc cagcgataac gcagtaaact
    35101 tcgggcaacc gtcggatcgc taaacagatg aaacggtaac aaaaatactt ctgtatccca
    35161 gaaaacgtgg cctttgtagc cttcgccggt taagcctttt gccgcaatgc tgctgcgctc
    35221 gtcgtgggca ggcgtcatga tgcgcagatg ataaagcgca taatctaacg cttgctgatc
    35281 gtgcgcttcg ccgccattta ccgtgatacg acgtttctgc caccattgac gccagttttc
    35341 tgttgatact gcaagaagtt ggtcgtaact ctgctgcgcg cacatttcaa gctggcgaag
    35401 cgacgcgctg ccccactcgt ctaaaacggc ttgcctgtca tcccgccagt cgatccacac
    35461 cagtttttgc aacgtcactg tctcgcctgc atgaagctgc gcactggtat gttgctgcaa
    35521 acggcgctct ttggcggtat agcattgctg cacatcaccg ctcaccatac agcaacagct
    35581 gatggccaca tcactgcgtc catcctgggt ggtgtagatc ccctgcatca gatgctgacc
    35641 aaacacccgc acctgggttt cgtcgagatg ttggcgaccg tggttggttt gcgtggcgtc
    35701 gatacctgtt gaaatcagca ctgacgcgtc ggcgtccagt ggcgtaatag tgatttccag
    35761 cacaatgagc ggcagttggt ctgccgaaac aaagcgacgg ctggcgatgg tgtaacctgt
    35821 gccgttgctg gtacgccaga caacgctacg gcgtaattct ccactggcaa agtcaagttc
    35881 acgctgccag gcttcgcggg ataacgagaa aacctcacca tttatggcaa tctccatccc
    35941 caagatatca ggcaggttca ccagttcgtt gatttcacct tttcccgccc gatgatacag
    36001 ccccgccaga tacatccctc gcgtctggcg cgtgtaatct tcttcatggc tggcgcgaag
    36061 cccaagatag ccgttcccct gcgccatcag cgatgcatac ttgttcaggg tatgctggct
    36121 gaaatggggt tctgataacg ttactggcct ggtcataatt ccacacgcgt ccctgtttct
    36181 gcggattgat acaacgccgc caccagttgc tggatgatgt acccctgctc ggcgtcggct
    36241 atcatcacgg gcttgccctg tacgtgattg ataaaggctt ccatgctgcg caaatggcgg
    36301 ttgtcgtctg ccagttcccg ttgcatcagc gtcattaatt caccgttgtt atcggtgtag
    36361 atatgtgctg gaaacagcgt cgcaccggct ttatcaccac aaaagctgac gttcataatc
    36421 gactgttcgc ggatgttgag tgcaaatgac gtttccagcc acagaatgcc gccgttatga
    36481 aattcaatgg tgccaaataa cgaatcttcg acgctgtaag ttgccggatc ccactcgcca
    36541 aattgaccgc agctcttttg cgtgccgatc ttttgaaagc tatgcgcatt cacgcttttc
    36601 accgccggaa agcccagcac atacatcgcc gcatccagca tatgaatgcc gatgtcgata
    36661 agcgggccac caccctgcag ttctttattg gtaaagacac cccagccggg aacgccgcag
    36721 cgacgcaggg cgcgggcggt ggtaacgtaa atctctccca aaacgccgtt ggtcacctgt
    36781 tcacgcagct gttgcgtatc gagcgcaaaa cggtggtgaa agtcgtaggc cagcaccttg
    36841 cccagtttgc gcgcggtatc gcacatttcc cgcgcctgtt ctggcgtcat ggcgggcggt
    36901 ttttcgcaca tcacatggca gcccgcttcc agtgccatca gggtatgttc gtaatgaaaa
    36961 cggttaggtg agcagacgct aaccacatca ggtttcaccg ccagcagcat ggcctgcgga
    37021 tcgtcccaca cggatgcatt accgtattta tctgccagcg cctgcgcctg ggaaaggcgg
    37081 ctgtcacaga cagccaccag ttccagatcg ttgcgggtgc agtagtacga agcatgaacc
    37141 ttatccgcca cctggcctgc gcctattatc gcgacccgca gcggagagct tgtcattgca
    37201 cttttcacaa agacctctta gcaggtacgc aaccaggcca acgaatcacg gtacgcctgg
    37261 gcaggctctt ccgcccggat acgcccttcg tacactacat agccctgata gttatccgcg
    37321 cgcagctgtt caaacagcgc gtggaaatcc agggtgccgc tgcccggctg gtagcgatgg
    37381 ttatccgcaa tatgcacatg accgagcagg tcgcggttgt catgcagcgc ctgcgccagg
    37441 ttatcttctt cgatattcat gtgatagaaa tcgccgataa tctgtacatg tttaagatcg
    37501 ttttcgacga tgtaacggcg ggcatcggcg agggtgttga tcatatgatc ctgatagcgg
    37561 tttaacggtt cgagatacac cacggttccg gtacgcgcgg cgacctgttc cagtacgtgc
    37621 agggaatcac tcaccatttt gcggtcgccg tccaggctac gcggcgaggt catcggcggt
    37681 aagcggaagg taaacatgcc ccacgctgcc ggaacgacga tacctttacc ccccacttct
    37741 gccagcgctt cgagaatgcg ttcgatctgc tttaagccat taagacgacg ctcttcgata
    37801 aagtcgccaa tccaaccgtc atagccaccg caggcggtgg tcaccggtaa accggtttct
    37861 ttgattgctg ctttgacttc ttcgagattg ttgaccagca gtttgccgtc aatctcaaaa
    37921 ccatcgaagc ccatctcttt gatataacga aatttctcca gaatgttttc cggaaagaat
    37981 gcctgattct gtgtgcctat tttcattttt tcattccttt gaattaaaac gtaacgccca
    38041 ttttgatgct ctgttccgga tgctgatcga catactgcat gtagctttcc gggctcttgg
    38101 caaaggtcac taccgggtcg attaaatctt cgcaattgag ataaccgttc atcagcagtt
    38161 cccagcaggt ttcttcaata cgcttacggc tccagcgcgg ataatccggg ttcggttcgc
    38221 tgcacgcacg ggagaaaaca attttggcgt tattgaaatg cgcttcgcgt ccgaggttaa
    38281 aaccttcagc aaatggtttg gcaaacgcga cataagagat ggtgccgcca taagcaaggc
    38341 cacgcagcgc cgattgcagc gcgtcggcgt aaccgctggt ttcgataatc acatcggcac
    38401 cctgcttgcc ggtcagcgtt ttgatctctt ttcctacgtc agtgccaatg aggttaaggc
    38461 agaaatcagc accgtggcga cgggcaatat cacagcgata ggcgatagga tcgacgccaa
    38521 tcaccaccga cgcgccagcg cgtttcgcca gttggatggc tatttgacca atcgcgccaa
    38581 gccctaccac caccacaaaa tcacccacgc gcacgttggc atcgcgcacg ccgctcatgg
    38641 caaactgcgc cgggtcgtaa caaacggcgt ttttccacga gctgccttcc ggcattttgc
    38701 gcagcttgta gttattcacc gcgttaatga tgaccgtctc ggagagcggg ccgtagccgc
    38761 atacgctgtc gcccaccgcg tagtcggtaa cgtcgctgcc gcactcgata atgtcgccaa
    38821 ccaccatgtt gccaagctgg aatttgccaa actcaatgcc gcgcggcgca tctgccggac
    38881 gcggagtgaa catctgccat tcgccgttaa agtcttcatc gataaacggg ctggcggcgc
    38941 ggaagtcgac cacttccgtt ccgtgtttcg gtgcgccgaa acgcacgcgg attttcactt
    39001 cattggctaa aatcgcccga tcttcatact caaccagcgc agcaacacgc ggtgctgtgg
    39061 ctactaactt tttcatttaa aactccttgt aaagaaactg gccgctttag cccttcacgc
    39121 caccggcggt caaaccactc ttaataaaac gttccgaaag cgcatacata atcaccaccg
    39181 gcaatgcggt caccagtgag gccgccatca tccgtcccca gatgtaatct ggcgtactga
    39241 acagcgcgtt caggcccacc ggtaaggtga aattgctggc gctggagagg aaaatcgacg
    39301 caaacagata gtcgttccac gccaccataa agcagtagac gaacacggat atcagcccgg
    39361 acatcgccaa cggcacggtg atgcggaaga taatttgcag acggttgagg ccatccatca
    39421 tcgccgcttc ttcgatctca tcggggatgg tgtcgaagta gcttttcagc ataaacacgg
    39481 cggtgggcag agtttgcgtc accatggtga tgatcagcgc catctcggtg tcgtaaatgc
    39541 ccagcgcggt gatgattttg aacagcggca ccaccagcaa aatgccagag aacatataca
    39601 ccgtgtaaaa gctggcattg atggtcatcc gacctttaaa gcgcaggcgg gaaagcgcgt
    39661 aagccccaag aatgccgagg aataccgcca ccacggatga aaccaccgac accaccagac
    39721 tgttacggaa gtagtcgaca aacgggaaaa tcatcgggtt aaaaatgtcg acgtaatgct
    39781 ccagcgtcca ctgctgcggc agcagcgtag gatgcagtga gatcgcctct ttcgcgccct
    39841 tgaacgaggt catcagcatc acaaaaaatg gaaacagcgt gatgatgaga aacagcgcca
    39901 gcccgcagta aaaaccgatg cgactgagtg tgcgtttatt tgttgccatt gaggttcacc
    39961 cgtttcctgg tcagcagaat gacagcgaaa ataatgatga agagcaccac cgagatcgcc
    40021 gccgcttttc ctaaatcatt aaaggcaaag gcggttttgt agagatatac accgagaata
    40081 tcgaccttgg tcgtcagcaa ataaacatcc gcgaacatgt agaacatcca gatggtgcgc
    40141 agggtcacca ccgtcgccag gaccggcata atcgcgggca gcgtgacgat acgaaaccgt
    40201 tgccaggcat tagcgccatc catctctgcc gcttcgtaca gcgatttgtc gatggtctgc
    40261 aaaatcgcca gaaacgagat aaaggcatac gggaagtagc gccagatggc gaacagcacc
    40321 accagcacaa agctactgcc cggattatcg aaccacagcg gtgcctgctc atagagatgc
    40381 agaagatcga cgccgaggta gttcacaatg ccgtagccgt tgttgaacat gtatttccag
    40441 gcaaacacca gcgaaatgga cggcgttacg taggagagga tcaccagcga acgcgcggtt
    40501 ttgcgcagac ggaactcgcg gttgaaaaac atcgccactg ccagcccgag cacggtgctg
    40561 cccgccacca ccagcgcggt ataccagacc gtcatccaca gcgagtgcca gaagccggga
    40621 tcggagagga tacgcacata gttactcacc ccaacaaagg ttgactcgat gttgggattg
    40681 agcggcagac gtaaaaaact gatttcgata ttcgacacca ttggccaggc caccagacca
    40741 cccagcagta ataagctggg cgcgagaagc agcagcgcaa agggcatatc ggaacgacct
    40801 gaaaaaagcc tgttcattgc ggattcctgt cctgggttta gtgctgttcg atcagttcat
    40861 ccagcttttt ctggctcgct tgcagcgtgc cagggagatc ggctttaccc acggtgacgt
    40921 tatgcaccat ggagctcacc acgccagacc ccgtcacatc acccatgcgg gtaaagtttt
    40981 tatcccctac tgcaccaaag acctgaatat ttggcagttc accgattagc tgattcggta
    41041 gttcacccag cgccttaata acgtcgttgt ctttccaggt ggcggtagtg acaaccgctt
    41101 tattcaccgg cagcgcagca cctggcgaca tcatcaccca gtcggcaatg ttgtctgcct
    41161 gctccataaa ggtgacaaat ttttctgctg cttccgtctc ttcggtcttt tgcccggcgg
    41221 taatggtcag cgaggtcaac atgccgtaga ccgcagagtt tttctcggtt ggcaccacga
    41281 aaccgacgtt tttcgggtcg ccttctttaa tcacagccgg aaggatatag gtggagtaaa
    41341 tcgccatcgg cgcggtgccg ttcataaagg cgtctttcac ttccatgatg tcgttagaac
    41401 ccggcatagt gttggcagca aggtcgcggt aataggtcag tgcctgcatc atctctggtg
    41461 tatcaagggt gattttgcct tcggcgttaa agacgttagc ctggttggat aacgcaaact
    41521 gggagaagga ttgttccgtc aacacgcttt ctgctgtagg cagcgcaatg ccgtattttt
    41581 tattcgccgg gtcattcagt ttctgtgcaa cgtccagcag ctgttgccag tttttcggct
    41641 cctcaagccc cgcttttgcc agcacatctt tgcgatacca gataccgcca atccaggcgc
    41701 tgacaggaac accggtccat gcgctaccat cttcggtacg cacaatacgc agtacgccat
    41761 cgtaaaacgc gccttcacca acgttgctga tgactgtggc aaccgctttg cgatcgataa
    41821 gctgctcttt gtccatcact ttggcgtagt catggctggt ttctatcact tccggcagcg
    41881 aaccgctacg tgaaagagta atgactttag tgttataggc atcttcttcc acgggcactt
    41941 gcttaacggt gatgccaggg ttttcttttt caaaacgggc aatcaattta ctgataacgg
    42001 cctggcgctc ctgctccacc gaagaatgca taaattcgat ggatacattc gttttatttt
    42061 cttctttaca ccctgaaatc agggcgcagg aaaccagtgc tgataacagc acgatttttg
    42121 atttaatcat gagatgttcc tttttattta atccacataa cctgccaggg gcgcagcgtt
    42181 atttcattac ccaatatatc aacttcacta atcagatccc gaccatgcag attagtaata
    42241 tttacatgct gaatattttt actgacatta aacaatccag tcagacaatt accatcagcg
    42301 ttacttcttt gaatacgcat tacggatgaa ttaatcgtat cgatggtaaa attattatcc
    42361 ggatgaaact cgttatggct gcgacgaagt gtaattaaac gcgacaactc atgatatacc
    42421 gcatgcctta atgtagcttc atcgttcagt tctcgggtta tctctttact gtgatatttt
    42481 ttacggttaa tcgcacggtt atatccgagt ttttcgacac ctgcgtaatc attacgcgag
    42541 ccaagaatac tttgaatata tatcgctggc acaccgggga aacttaacaa aatcgcatgg
    42601 gcgagaataa acctggcgca acgttcttca tcgctactct cacggcggct taacgcatcc
    42661 atataggtca catttatttc atacggactg cgtgtaccgt cgggattatt tttccagttt
    42721 actaatgcac cttcctgctg taacgcctcg accagctcta atatttcgct ttcaggcaac
    42781 aagccccgta gcgggtttag cccgatgcca tcgtgagagg cgaggaagtt aaaccaggtg
    42841 gtgttgctgg aaggtagtgt caggttttgc gcccacgcac aaagcgcctc aacgttctgt
    42901 ttttgcaccg catgcagcac cagtggcggc agcgagaact ggtacaccat atgtgcttcg
    42961 tcatcgcctg cgccaaagta agcaatgttg tctttatgcg gaacattggt ctcggtaatg
    43021 atcactgtac ctggcgcaac gttatcaata atcgaccgta acagtttgat aatcagatgt
    43081 gttttttcca gatggatgca gcttgttccc ggctctttcc acataaagcc aacggcatcc
    43141 aggcggacat actcagcacc tttcgcaagg taacagagta aaacatccac catcgccagc
    43201 aacacttcag ggctacggta attcaggtca atttgatcgt cactaaaggt ggtccataaa
    43261 tggcgcgttg aatgatcgcg catctggaat ggcgttaata acggtaacgc acgcgggcga
    43321 gtgacggcgc tgagatcggt ttgcgggtca acggcaataa aaaaatcttc aaaacctgga
    43381 tgctgttgta aatagttttt aaatcattca ctttttgccg acatatggtt gcagacaaaa
    43441 tcaaacatta aatgactgca ttcaccgagt tgctgaatat cctgccactc ccccgcttca
    43501 ctggcgacct gatgataatc aattaccgaa aagccatcat cagatgacca tggataaaac
    43561 ggcaacaaat gaacatgtga aaaaatgctt tgcagccatt gatggtaaaa ctgattaaat
    43621 gtgggtaatg gttttaaatc attgctgtga aattgatcgg cataggtaat taacacgaca
    43681 tcactttcat cccagtgttt tttacgtcgc tgtgtaatta atcgtttcgc actctcaaga
    43741 cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc caccgtagat ttcgtccagg
    43801 taatccgtaa ttttctgttt catgttgcct ttccgtggta gcggtcccat gaaacgagag
    43861 actactccgc agaaaataaa ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc
    43921 attttttacc agggaaaaag cgtatttgtg agctgacgca aataatctat ggggatgtaa
    43981 ataaggtgtg acagaaaaaa aacggcgcat aagcgccgct catggtgaat tcttttaacc
    44041 tttgaccttc ggcattgcga tgtctttcag gccaccggcg ttctcaacgt gggtatatcc
    44101 catctcgcta aggatctctt ttgcttgccc tgactggcgt ccggcattgc aatacacttt
    44161 cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc tctttcactt ccttcagggg
    44221 aatattgatg gccccctgaa cgtgctcttg ctgatactgc tctggaacac gaacatcgat
    44281 ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt gccagagcaa gtaagccttt
    44341 tttaaacatg taagcgtcct tctatggatg aaaattgcca tccattatac gcctgagatt
    44401 taacaatttt tgcgaaactc ccctcacctt ttataacgct gtgccagttt attagcagcc
    44461 cgacttaaca gcggttccag cgccacagcc agcaacattt tcagcggtcg gcgagcaact
    44521 gattttatcg cccagcccgc tacacccgcc gggccatagc gcagtgcggt aagaagtacc
    44581 agcttgcctg ctaatttgaa accaggcttt accttttgcc cggcctgttg ccagcgagta
    44641 ttcataactt tcctcacagt tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga
    44701 cataacgttc catctcgcgt aaacgcgttt cacttgccgc cagttcacgg tcgacttcat
    44761 ccagcaattc gctgctggaa ggtagctgct caccaaaggc catgttgtcc ggcattggat
    44821 caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa
    44881 tcgacagcac caccaggata cgcaccagtt ttgccggtac atcaaaatag ttggcaatcc
    44941 cggcgcagac gccgcggacc atgccctgct gtggaatacg ccataatttt ttattgagat
    45001 taatgcccgc cattagcgat ccctccagtt cggatgttct gcatcaagaa tagattccag
    45061 cgcctgaata cgttcgcgca tccgttttgc ttcatcagcc agttgcgcta atcgctgctg
    45121 ctcactttgc gacaattcac tgcgaccaga acgattgctg taatgcagcc ataaccagat
    45181 cggtaaaaca aacagcacaa aaatggttaa cggaatagcc agaaatagcg cgctcataag
    45241 tactccttag acgatgagcg gcggcgcgtt cggacgccgc ctggatgtta ttattgattg
    45301 tcttgcttca ttttggcttt taattgtgcc agttgttcgc tgattgcatc atcggctttc
    45361 agttcggcaa actgatcgtc cagcgatttt tgtttaccga agctgtggct ttctgcttcc
    45421 gcttccatct ggtcaatacg acgttcgaaa gattcgaaac gagccattgc ttcatccagt
    45481 ttgccactgt ccagctgacg acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac
    45541 atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt tttccagctc accgatctct
    45601 ttcttcatgc gtgccagcgt gtcgtccacc agcgtcactt catgttccag gctcttaatc
    45661 agatcggtca gtttctgttt ttcaattaac gctgcacgtg ccagatcctc tctctctttc
    45721 agcagcgcca gttcggcttt ttcctgccat tcaacttcac gcgccgacgc ttgttcaata
    45781 cggcgagtca gctgtttctt ttctgccaac gcgcgcgccg aagtagaacg tacttcaacc
    45841 agtgtatctt ccatctcctg gatcatcaga cgaaccagtt tctgtggatc ttccgctttc
    45901 tctaacagag cgttgatgtt ggcattcacg atgtcggcaa agcgagaaaa aatacccata
    45961 atgttgtcct cttgatttct gcgttcagga ttgtcctgct gaactgttaa tacaatttgc
    46021 gtgccaattt tttatctttt tgatttataa agatctgatt gaagaaccaa cagcaacatg
    46081 ccaggatgag ttagcgaatt acactaacaa gtggcgaatt tcatcatggc agaatacaaa
    46141 gataatttac ttggtgaggc gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc
    46201 gcaccgctgg acaaaccggt gctcatcatc ggcgaacgcg gcaccggtaa agagctgatt
    46261 gccagccgcc tgcattatct ctcctcccgt tggcaagggc cgtttatttc ccttaactgc
    46321 gcggcgttaa atgaaaatct gctggattcc gaactgtttg gtcacgaagc gggggcgttt
    46381 accggtgcgc aaaaacgtca tcctgggaga tttgaacgtg ccgacggcgg tacgctattt
    46441 cttgatgaac tcgctacggc accgatgatg gtgcaggaga aattattgcg cgtgattgag
    46501 tacggtgaac tggagcgcgt tggcggcagc cagccattgc aggtgaatgt gcggttggta
    46561 tgcgcgacga atgccgatct cccggcgatg gtcaatgaag gcacttttcg cgctgacctg
    46621 ctcgaccgac tggcttttga tgttgtacaa ctgccaccac tgcgcgagcg cgaaagcgac
    46681 ataatgttga tggcagaaca ctttgccatc cagatgtgtc gggaaattaa gctgcctctg
    46741 ttcccggggt ttacagagcg cgccagagaa acattgctga attatcgttg gccgggaaat
    46801 attcgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc gccacggcac cagcgattat
    46861 ccgcttgatg acatcattat tgatcccttt aaacggcgtc cgcctgaaga cgctatcgcc
    46921 gtttcagaaa ccacctcgct tccaacactg ccgctggatt tacgtgagtt tcagatgcag
    46981 caggaaaaag agttgctgca actcagtttg caacaaggga aatataacca gaagcgtgcg
    47041 gctgaattac tggggttaac ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt
    47101 tagcgaagtt gagcaataca acttgccgga tgcggcgtga atgctttatt cggcatacat
    47161 aatcaatacg tcgtttgcag gcctgataag cgcagcgcat cagacattat tgcgttgggc
    47221 tattaatcgc tcagcgcatc ctgcaaaatt ttcattgccg catcgaattg cgcatccggt
    47281 atggtcagcg gatacaggaa gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc
    47341 agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc
    47401 gtttgcggat cgttaaactc tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg
    47461 gccggaacgc tttctttggc atcaatcaac gtgtttttga gacgctggcc cagttgattc
    47521 gcgcgttcgc agagtgattc tttgtcgata atattgagca ccgcgtgcgc ggcagccacc
    47581 gccagcgggt tcccggcgta ggtgccaccc aacccgcccg gcgcgggcgc gtccataata
    47641 ttcgcgttac cgaccacgcc cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc
    47701 attaagtccg gcttatcggc gtaatgatcc atggcaaaca gtttaccggt acgcgcaaag
    47761 ccgctttgca cttcatcggc aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg
    47821 gcggcaacca gctcttttgg cgcaacgttg aaaccgccct cgccctgcac cggttcgaaa
    47881 ataatggccg cgacctgctt cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg
    47941 agggagtcct gtgttgaaat gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa
    48001 ccagggaacg ggccgaagcc gattttgtac ggcgcaactt ttccggtcag cgccatggtc
    48061 atatatgtac gaccgtggaa gccaccgcta aacgcaatca cgccaggtcg tccggtatgg
    48121 gcgcgagcaa ttttcaccgc gttttccacc gcttccgcac cggtagtgaa gaatgcggtt
    48181 ttagcctgcc cgctcactgg ggcaagggcg ttaattttct ccgccagcgt gacgtagctt
    48241 tcgtacggca caatctgata cgccgtgtgg gtaaactgtt gcaattgctg ctccaccgcc
    48301 gcgaccagat cagggtggcg atgcccggta ttcagcaccg caatgcctgc ggcgaaatcg
    48361 atgtattcgt tgccctctac gtccttcagc gtagcgtttt ctgccgactg ggcgaagaag
    48421 ttacacatca cgccaacccc gcgtggggta gcagaaagac gacgctgatg gaattcattg
    48481 ttgctcatgt ttcacacctt ttgactcttc actgttaatc ggaaattaaa cgcccagacg
    48541 atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaat ggcacgcgca gcgtgcgccc
    48601 gccggggaac gggtaatgcg gcagattggc gaaggcatcg aaacgttcgg cgtcgccgcg
    48661 cagcagttcg gcaatcagac gcccggcaag atgggtacag gtgacgccgt ggccgctgta
    48721 gccctgcatg taatagatgt tggtatcaag gcgaccgaac tgcggcatgc gcgacaaggt
    48781 cagcaggaag ttgcccgtcc agcggtaatc aattttcacg cccttcagct gcgggaaggt
    48841 tttcagcagt ttcggcacca caaggcgctc aacgtcatcc gggtcgcgcg cgccgtagac
    48901 cacgccgccg ccgtacagca ggcggttgtc ggcggtaaga cggtagtaat caagcagata
    48961 gttacagtct tccacacagt agtttttcgg gatcagcgaa cgggctaaat cttccgacag
    49021 ccgttcggtg gtgatcacct gcgtgccgca cggcatgctg cgtttcgcca gttccggctc
    49081 tactttatcg cccagatacg cattcccggc gacaatcaca tacttcgccg tcacctgacc
    49141 tttggcagtt cgcacaacgg ctggcgtggt gtgctggatc tgcgtcacgg cagaaagttc
    49201 atacacgcgc ccgccgttga ggcggatggc gtccgcttcg ccaatggcga ggtttagcgg
    49261 atgaatatgc ccaccgctgt gatccagcag cgcaccggta tagcgatcgc tggctacttc
    49321 acggcgaatg gcgttggcgt ccagcaattc cagctgttta ttgccgtagc gttcccagtt
    49381 ctctttctgc tcttcaagtg tggcgagctg tttatcattc atcgccacaa acaggccgcc
    49441 ggggcggtag tcgcagtcaa tctgataacg tttgatacgt tcgcggatga tctcaccacc
    49501 ttcgaacatc atgctgccga gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac
    49561 gtcgatgtcg cggctgtagg agttcacaag ctgcccgcca ttgcgcccgc ttgcgccaaa
    49621 gccgatgcgt gaggcttcga gaaccactac gtcaaagccc gcttccgcca gatgcagcgc
    49681 ggaagagagc ccggtatagc cgccgccgac cacgcaaacg tcgcaggaaa tcgactcatt
    49741 cagcgtgtcg aatggcgcgt atttattcgc actggcggcg tagtaactgc tggtatgttc
    49801 ggtcatttca ggcctccagg cttatccaga tggttttcag ttcggtgaat ttttcaaggg
    49861 catgcaggga tttgtcgcga ccgttgccgc tctgcttata gccgccaaac ggcacggtca
    49921 tatcgccgtc gttgtagtta ttgacgaaga cggaaccagc cttcaggcgg cggctcatgc
    49981 ggtgcgcgcg ggagaggtcg cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt
    50041 tggcaagctg tagcgcctgg tcttctgatg tgaaacgcgt gaccaccagc accggaccga
    50101 aaatctcttc gcggcttaag gacgcattcg ggtctacatc cacaaagatg gtcgggccga
    50161 tggcggcagc cagctcagcg ttacgaccat ccagcagcag ttgccctttg ctttcgcctt
    50221 cccgaataaa gctatggacc gagtcagcgt gagcgctgtc gattaaggtg cccatggtgg
    50281 ttgcgggatc aagtggatgg cccggctgcc agttttgcgc ctgctgtttt aacagggcta
    50341 agaattcatc ggcgatgctc tcttccagta acaaacgcgt tccggcgatg cacacctggc
    50401 cctggttgta gaagatgcct gcggcggtgg cgcttgccgc tttttgcaga tccggacaat
    50461 cggcgaaaac gatgttggcg cttttgccgc ccgcttccag ccagacgcgt ttcatgttgc
    50521 tctcgcccgc atctttcagc agctgtttcc cggtacgggt cgaaccggta aaggcaatgg
    50581 cgtcgatatc gttatgacgc gacagcgcct gccccgcttc atgaccaaaa cctgtcacca
    50641 cgttcaaaac accatccggc aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac
    50701 tgagcggtga tttttcagac ggtttcagga caacgctgtt ccctgctgcc agcgccgggc
    50761 caagcttcca gcaggtcagc agcagcggaa agttccacgg tacgatggcg gcaatcacac
    50821 cgaccggttc acgcacgatc atcgccagct catggctact ggtggtcgcc acttcgccat
    50881 acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat
    50941 cacgcagact gtgacgaatc ggtttgccgg tgtcgagagt ttccagcagt gccagctctt
    51001 cggcgtgggc ttccattaaa tcggtgagtt tattcagtac cgctttacgt ttagccggag
    51061 aagagagtga ccagtcgccg cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga
    51121 tatcgacgct cttgccgcgg gcaattttcg ccagcggtgc ctgggtgacc ggatcaacgg
    51181 tttcaaaggt ttcattttcc gccgcagcag tatattcacc gttaataaat aagcgggttt
    51241 caatggcgag acttaacgct ttatcctgcc agtaagccag atgatgaaaa ttcattatga
    51301 ctcctgtttc acgtctatca gatatatgca gtttgtgaac gtcagggcgc agagatcccc
    51361 gggccgtatg cccgtttatt tctggcgaca ttctttatat acacttcatc cttcaagccg
    51421 cctctgcctt ggctgcgttc gctcccccga atcacttacc tgagtaaact catcgggatt
    51481 cgctcacttg ccgccttgat gcaacttgaa tgattttgtg tatagattgc cgggcgcatt
    51541 gcgcttaccc ggcttacaga acaaaaagat taaaacgtgg tgggcgtatg ggcgctgata
    51601 attcggcaaa tacctgccga cgtattactg aaactgtgcg ggatgccggt attaatggca
    51661 taactttgcc ccgcgacgag gtggtaatcc tgaccattaa tcgtcagaac aatttcacct
    51721 tccagtactg tgcctatttc ctcaccctga tgcttaattc tttccccagt ggttgtgccc
    51781 ggctggtacg tttcaaagat catcgccagc gtgcgattcg ggttaccgtt atgaaccagc
    51841 ttcattgaca caccctgact gcccatctca attaagtcgt cctgattaat gacgacctgc
    51901 ggctcatcag gtttttccgg ctcggaaaag aattccgaga gtgacagacc atacaccttc
    51961 agcagctttt gcagcgtact gatggcaggg ctgactttat cttgttctat cgtactgata
    52021 gcactgtgag tcagcccgga gagttcggcg gcacgacgtt gtgaaagccc ctgctgctgg
    52081 cggatttccg acaagcgttt tcctggcgcc agtccctcat cactcatatt tgcatttcct
    52141 taaactgtag tggtcagagt cgctgttttt cagcgatatg gtgctgacaa gcggtgataa
    52201 agccctcgaa caatatacgc gaaagcgcgt actcgctact gttccattcc gggtgccact
    52261 gtacgcccag cgcaaaagga tgattgatga cgctaaccgc ctcaactaag ccatccggcg
    52321 agcgagcttc aacacgcaac cgtgggctaa cgaccttcgc cccttgccca tgcagagagt
    52381 ttacccaaaa gttgctacat tcaggtaaca acgcagacag taatcccccc tcttcaacct
    52441 gaacttcatg cgacggtgca tattgctgtt ccaccggcag ttcgggatct tcccgatgtt
    52501 ccagcaattc aggctgttcg cacagcttgc gatgcaacga accaccggtt gccaccacca
    52561 gttcctgtaa accccggcag atggcgaaaa tggggatgcg cctttcgagt gcggcattaa
    52621 ttaacgccat gctcagaaga tcacgcccgg gatcggcgtc aggctcatcg ccgttttcac
    52681 catataggtg cggctgcaca ttgctgggac taccaggaag ataaatgcca tcgagtttcg
    52741 gcaaaagttg ttcaagtaat gacggttccg ccagcgcatg tggtagcgca attggcaagc
    52801 cgcctgcgtg gatgatggca ttcaggtact tttcttgcag agtctgggtc gcatgaccct
    52861 taagcctgtt cctgcacatt acgacaccga taaccggatt gttcattata ttttccatgc
    52921 tcaatctcac aaagtggact aaattatcgc cattactgcg gtttaaatga cctttatgtt
    52981 caatattttt tcaatctagc agtgggtttt catttttgca aactcaattt aacatttgac
    53041 aaacatttag tttgcataca gattcgaatg gtggtcatta tattttacgc tttgataacg
    53101 agcggaaaac aaaccaaagg cgaagaatca tggaaaccaa tatcgttgaa gtagagaact
    53161 ttgttcagca gtcagaagag aggcggggta gcgcctttac gcaggaagtg aaacgctacc
    53221 tggagcgcta cccgaatacg caatatgttg atgttttact tactgatttg aatggttgct
    53281 tccgtggaaa acgtatccct gtttcaagcc tgaagaagtt ggagaaaggg tgttatttcc
    53341 cggcctcggt gtttgcaatg gatattctgg gcaacgtagt agaagaagcg ggtctgggtc
    53401 aggaaatggg cgagccggat cgtacttgtg ttcctgttct cggttcctta accccttctg
    53461 ccgccgatcc agagtttatc ggtcagatgc tcctgaccat ggtcgatgaa gatggcgctc
    53521 cctttgacgt tgagccgcgg aacgttctta accgcctgtg gcagcagcta cgccagcgcg
    53581 gattgttccc ggttgtagcg gtagagctgg agttctattt actggatcgt cagcgcgacg
    53641 ctgaagggta tctgcaaccg ccctgcgcgc ccggcaccga tgaccgcaat acacaaagcc
    53701 aggtttactc cgttgataac ctcaaccact tcgccgatgt gctcaatgat attgatgaac
    53761 tggcgcagtt acagttaatt cccgccgacg gtgcggtcgc cgaagcctcg ccgggtcagt
    53821 ttgaaatcaa cctttaccat accgataacg tgctggaagc ctgcgatgat gcgctggcgc
    53881 taaaacgcct ggtgcgtctg atggcagaaa agcataagat gcacgccact tttatggcga
    53941 agccgtatga agagcacgcg ggcagcggaa tgcatatcca tatcagtatg caaaataacc
    54001 gtggcgagaa cgtgctttct gacgcggaag gcgaagattc gccgctgctg aaaaagatgc
    54061 tcgccgggat gattgacctg atgccgtcgt cgatggcgtt gctggcacca aacgtgaact
    54121 cgtatcgccg cttccagccg ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca
    54181 accgcaccgt cgccctgcgt attccgtgcg gcgaccgtca taatcaccgc gtggaatatc
    54241 gcgtggcggg tgccgatgcc aacccatatc tggtgatggc agcgattttt gccggtattt
    54301 tgcatggcct tgataacgag ctgccgttgc aggaagaagt cgaaggcaac gggctggaac
    54361 aggaaggctt acccttcccg attcgccaga gcgatgccct gggtgagttt atcgagaatg
    54421 atcacctgcg ccgctattta ggcgaacggt tttgccatgt gtatcacgcc tgtaaaaatg
    54481 atgagctgtt gcagtttgag cgcctcatca cagaaacaga aattgagtgg atgttgaaaa
    54541 acgcgtaatt ccccatgcgg ggttgtttca tcggcctgag cggagccagg cctgacattt
    54601 gctggtaatc cagacatttc ccggatgtcg cgttatggag cgcaggcggc aacgggcttc
    54661 cgttaacgat taagactgtg acgaggaaat gagatgatgc cgatgattaa atccccgcat
    54721 ggcgagggag gctgcgtatg cgcccctcct gctactgact ggactccgcc ccctttgctt
    54781 cccttgctaa acaggtttga tttcaggtcg acacgaccgc aaaccttatt acgcagggga
    54841 ggcagcaatt atggctatta attcaccact gaatattgct gcgcaacccg gcaaaacccg
    54901 tctgcgaaaa tcactgaaat tgtggcaggt ggtaatgatg ggtctggcct atctcacgcc
    54961 gatgaccgta tttgatactt ttggtattgt ctccggcatt agcgacggtc acgttccagc
    55021 gtcctatttg ctggcgctgg cgggcgtgct gtttaccgct atcagctacg gcaaactggt
    55081 tcgccagttt ccggaggccg gttcggccta tacctacgcg caaaagtcga ttaatccgca
    55141 cgtcggattt atggtcggct ggtcatcact gctggattat ctctttttgc caatgatcaa
    55201 cgttttgttg gcgaaaatct atctctccgc cctcttcccg gaagtgccgc cgtgggtgtg
    55261 ggtggtaacc ttcgtcgcca ttttaaccgc cgcgaatctg aagagcgtca acctggtcgc
    55321 taacttcaat accctgtttg tactggtgca aatctccatc atggtggtgt ttatcttcct
    55381 ggtggttcag ggactgcata aaggagaagg cgttggcacc gtctggtcac ttcagccgtt
    55441 tatcagcgag aacgcgcacc tgatcccgat tattaccggt gcgacgattg tctgtttctc
    55501 gttcctcggt ttcgatgcgg tgaccacgct ttcggaagag acgccggacg ccgcacgcgt
    55561 gatcccgaaa gccatcttcc tgacggcggt atacggcggc gttatcttta tcgcggcgtc
    55621 gttctttatg cagctgttct ttcccgatat cagccgcttt aaagacccgg acgccgcact
    55681 gcctgaaatt gcgctgttcg tcggcggcaa gctctttcag tcgattttcc tctgcaccac
    55741 gtttgtgaac acattagcgt ctggcctggc ttcacatgcc agcgtgtcgc gtctgctgta
    55801 tgtgatgggg cgtgacaatg tgtttccgga gcgcgtgttt ggctatgtgc acccaaaatg
    55861 gcggactccg gcactcaacg tcattatggt cgggattgtc gcgctgtcgg cgctgttctt
    55921 tgatttagtc accgcgacag cactgattaa cttcggtgct ctggtggcgt ttaccttcgt
    55981 gaatctgtcg gtgtttaatc atttctggcg gcgtaaagga atgaataaaa gctggaagga
    56041 tcacttccac tatttgctga tgccgctggt tggcgcgctg acggtgggtg tgctgtgggt
    56101 taacctagag tcaacgtcac tgacactcgg tctggtatgg gcttcgttag gcggcgcata
    56161 tttgtggtat ctgatccgcc gctatcgcaa agtgccgctg tacgaaggtg acagaacgcc
    56221 ggtgagcgaa acgtaatagc cggatgcgcg gcgcattatc ctgcctgagg gtaacgcgcc
    56281 gcgccattaa ctataaaaag atgtatgacc cggatcacga gtgctatcat ccgcccttat
    56341 gcttagcgca ttaaccccag gggcaaataa tgaaccacga tatcccgcta aaatattttg
    56401 atattgccga tgagtacgcg accgaatgcg cagagcctgt cgcagaggct gaacgcacac
    56461 cgctggccca ctacttccag ttactgctca cccgtttgat gaacaatgaa gagatcagcg
    56521 aagaagccca acatgaaatg gctgctgaag cggggattaa tcccgtacgc attgatgaga
    56581 tcgcagagtt tttgaatcaa tggggtaatg agtaattatt taattgcaga cgatcggtca
    56641 gtatcgttgt ccggataaag cgttcacgcc gcatccggct aacaatgaca cgcactgcag
    56701 cgtgctttaa tgggtttctc acgatcgtac cgctctgctc ctcgctaagt tgttctgtga
    56761 gttatagtta tagagaaatc acgggtacga atgttgaaca gatggcgacg ccacctgttc
    56821 aacgaccaac cacacggccc tcattacgac ccctgaacac aaacggtaat ggcaaatgtc
    56881 attaaatatt agtaagatag cctctttaca taaaatccct taatattatg cgccaggtat
    56941 tatcgtctct tttggtgatt gctggacttg tgagtggtca ggcaatcgcc gcgcctgaat
    57001 cccccccgca tgctgatatc cgcgacagcg gttttgtcta ttgcgtcagc gggcaagtca
    57061 acacctttaa cccatccaaa gcgagcagtg ggttaattgt cgataccctt gccgcccagt
    57121 tttatgatcg actgctggat gtcgatccct atacctatcg cctgatgccg gaacttgccg
    57181 aaagctggga agtactcgac aacggcgcga cctatcgctt ccacctgcgt cgcgatgttc
    57241 cgtttcaaaa aaccgactgg tttactccca ctcgtaaaat gaatgccgac gatgtggtgt
    57301 ttaccttcca gcgaattttt gaccgcaaca acccgtggca taacgtcaac ggcagcaact
    57361 tcccctactt cgacagcctg caatttgccg ataacgtgaa aagcgtccgc aaactggata
    57421 atcataccgt tgagttccga ctcgcccagc cggatgcttc ttttttgtgg cacctcgcaa
    57481 cccattatgc ttcggtcatg tcggcagaat atgcccggaa gttagagaaa gaagatcgcc
    57541 aggagcaact cgaccgtcaa ccggtcggca ccggtccgta tcagttgtcg gaataccgcg
    57601 ccgggcaatt tattcgccta caacgtcatg atgacttctg gcgcggtaaa ccgttaatgc
    57661 cgcaggtagt ggtggattta ggctccggcg gcaccggacg tctgtcgaaa ctcctgaccg
    57721 gggaatgcga cgttctggcc tggcctgctg ccagccagct atccattttg cgtgacgacc
    57781 cgcgcttgcg tttaacgctg cgtcctggga tgaacgtcgc ctatctggca tttaacaccg
    57841 ccaaaccgcc gctaaataat cccgctgtcc gccatgcact ggcactggcg attaataacc
    57901 agcgcctgat gcaatccatc tattatggta cggctgaaac ggcggcctct attttaccgc
    57961 gcgcctcgtg ggcctatgac aacgaggcta aaattactga atacaatccg gcgaaatcgc
    58021 gcgaacagtt gaaggcgttg gggctggaaa acttaacgct gaaactgtgg gtgcccacac
    58081 gttcgcaggc gtggaacccc agtccactga aaactgccga actgattcag gcggatatgg
    58141 cgcaggttgg cgtaaaagtg gtgattgtgc cggtagaagg tcgctttcag gaggcgcggt
    58201 tgatggatat gagccatgat ctgacgttat ccggttgggc gacggacagt aacgacccgg
    58261 acagtttctt ccgtccttta ctgagctgcg cggcaattca ttcacagacc aacctcgccc
    58321 actggtgcga tccgaaattc gacagcgtgt tgcgtaaggc gctctcctcg cagcagctgg
    58381 cggcgcgtat tgaagcctat gacgaagcgc agagtattct ggcgcaggaa ttgcccattt
    58441 tgccgctggc gtcgtcattg cgtttgcagg cctatcggta cgatatcaaa ggtctggtac
    58501 ttagcccgtt tggtaacgcc tcctttgctg gggtgtatcg cgagaaacag gatgaggtga
    58561 aaaaaccatg attatcttca ccttacgccg cattttgtta ttgattgtca ccttgttcct
    58621 gctgaccttc gttggcttca gcttgagcta tttcacccct catgcgccgt tacaaggcgc
    58681 gtcactgtgg aatgcctggg tgttctggtt taacggcctg atccactggg attttggtgt
    58741 ttccagcatt aacggtcaac cgattgccga acagttaaaa gaggtcttcc cggcgacgat
    58801 ggaactgtgc atcctcgcct tcggttttgc gctgattgtt ggcatccccg tggggatgat
    58861 tgccgggatt acgcgccata agtggcagga taacttgatc aacgccatcg ccttactggg
    58921 cttttcaatc ccggtgttct ggctggcgct tctgttgacg ctgttttgtt cactcacgct
    58981 aggttggttg cccgtttcag ggcgtttcga tctgctctac gaagtgaaac cgattaccgg
    59041 ttttgcgttg attgatgcct ggctttcgga ctcaccatgg cgggatgaaa tgatcatgag
    59101 cgcaatccgc catatgatat tgcccgtgat caccctgtcg gttgcgccaa caactgaagt
    59161 gatccgcctg atgcgtatca gcactatcga agtgtatgac cagaactatg taaaagcggc
    59221 ggcgacccgc ggtttgtcgc gctttactat tttgcgtcgc cacgttctgc ataacgcgtt
    59281 accgccggtt attcctcgcc tgggcttaca gttttccacc atgctgacgc tggcgatgat
    59341 caccgaaatg gtttttagct ggccgggttt agggcgctgg ttaattaacg ccatccgcca
    59401 gcaggattac gcagccattt ccgccggagt gatggtgtgt ggctcactgg tcattattgt
    59461 taacgtgatt tctgatattt tgggtgccat ggctaaccct ctgaaacata aggaatggta
    59521 tgccttacga tagcgtatac agcgaaaagc gcccgccggg cacgctgcgt accgcctggc
    59581 gcaaatttta tagtgacgcc tctgcgatgg tcggcctgta cggctgcgcg gggctggctg
    59641 tactgtgtat ttttggcggc tggtttgcgc cttacggcat cgaccagcaa tttctcggtt
    59701 atcaattatt gccgccgtca tggtcgcgct atggcgaagt ttctttcttc ctggggactg
    59761 acgacctcgg acgcgatgtt ttaagccgtt tactgagcgg agctgcgcca accgtgggtg
    59821 gcgcatttgt ggtgacgctt gccgcgacga tctgtggcct ggtactgggc accttcgccg
    59881 gggcgacaca cggcctccgc tcagcggtgc ttaaccatat tctggatacc ttactggcga
    59941 tcccttcgct gctgttggca attatcgttg tagcgtttgc cggaccgagt ttgtcccacg
    60001 ctatgtttgc tgtctggctg gcattgctgc cgcgtatggt acgttcgatt tacagcatgg
    60061 tgcatgacga actggaaaaa gagtacgtta tcgccgcccg tctggatggc gcatcaacgc
    60121 tgaatattct ctggtttgct gtgatgccaa acatcaccgc cgggctggtc accgagatca
    60181 cccgcgcact gtcgatggca attctcgata tcgccgcgct gggctttctc gatctcggcg
    60241 cacaactccc ctcgcctgaa tggggagcga tgctcggtga tgcgctggaa ctgatttatg
    60301 tcgccccgtg gactgtcatg ctgccaggtg cggcaattat gattagcgtg ttgttggtta
    60361 acctccttgg cgatggcgtt cgtcgtgcga ttattgcggg ggtggaataa tgccattact
    60421 cgatattcgt aacctgacca ttgaatttaa aaccggtgat gagtgggtta aagccgtcga
    60481 ccgggtaagt atgaccttaa ccgaaggtga aatccgcggt cttgttggtg aatccggttc
    60541 cggcaagagt ttgattgcga aagcgatttg tggggtgaat aaagataact ggcgtgttac
    60601 tgctgaccgt atgcgttttg atgatatcga tttgctgcgt ctctccgcac gcgaacggcg
    60661 caaactggtt ggccataacg tgtcgatgat tttccaggaa ccgcagtcgt gtcttgaccc
    60721 ttcagaacgt gtgggtcgcc agttgatgca aaacatccca gcctggacct ataaaggccg
    60781 ttggtggcag cgttttggct ggcgcaaacg ccgtgccatt gaactgctgc accgcgtggg
    60841 gattaaagat cacaaagatg cgatgcgcag ttttccctat gagttgaccg aaggtgaatg
    60901 tcagaaagtg atgatagcca ttgcactggc gaatcaaccg cgtctgctga ttgctgacga
    60961 accgaccaac tcaatggagc caacaaccca ggcgcaaatc tttcgcctgc tgacgcgtct
    61021 caaccaaaac agcaatacca ctattttgct tatcagccat gacttacaaa tgcttagcca
    61081 atgggcggat aaaattaacg tgctttactg cggtcaaaca gtggaaaccg cgccgagtaa
    61141 ggagttggtg acgatgccac atcatcctta tacccaggcg ctgatccgcg cgataccaga
    61201 cttcggcagc gcgatgccgc ataaaagtcg cctcaatacg ctgcccggcg ctatcccact
    61261 gctggaacag ttaccgattg gttgtcgtct ggggccacgt tgcccgtatg cacaacgaga
    61321 atgcattgtg acgccacgtt tgacgggggc gaaaaatcat ctctatgcct gtcatttccc
    61381 gctgaacatg gagaaagagt gagatgatcg aaacgctgct tgaagtgcgt aatttaagta
    61441 aaacgttccg ctaccggacc ggctggtttc gtcgtcagac cgtagaagcg gtaaaaccct
    61501 tgagctttac gctacgtgaa ggccagacac tggcgattat tggcgagaat ggttcgggta
    61561 aatccacgct ggcaaaaatg ctggcgggaa tgatagagcc taccagcggc gagttattga
    61621 ttgacgatca tccactgcat tttggcgatt attccttccg cagtcagcgt attcgcatga
    61681 tttttcagga tccttcgacc tcgttgaatc cccgtcaacg tatctcgcaa attctggatt
    61741 ttccactgcg cctgaacacc gacctggaac cggaacagcg tcgcaaacag attattgaaa
    61801 cgatgcgtat ggttgggctg ctaccggatc acgtcagtta ctatccgcat atgttggcac
    61861 ccggacaaaa acagcgtctg ggtctggcgc gcgcgctgat attgcgccca aaagtcatta
    61921 ttgccgatga agccctcgct tcactggata tgtcgatgcg ttcgcagttg attaatctga
    61981 tgctggagtt acaggaaaaa cagggcattt cgtatattta tgttacccag catatcggaa
    62041 tgatgaagca cattagcgac caggtgctgg tgatgcatca gggcgaggtt gtcgaacgag
    62101 gcagcaccgc ggatgtgctg gcatcgccgc tgcatgaact caccaaacgg ctgattgccg
    62161 ggcactttgg cgaagcattg acagcggatg cgtggcgtaa agatcgctaa ttttctgaat
    62221 cctccttcca gattatccac ctcattctga caagagatta aatcttgtca gaatgaggtg
    62281 atggataaaa taaaatcaaa tgcgcgtgat ttaagacgca atctcacttt gcaggaacga
    62341 aagctctggc gatatcttcg cagccgacgt tttggtgatt tcaaatttcg ccgtcaacat
    62401 ccggtgggga gctacattct cgattttgct tgctgctcgg cgcgcgtagt cgttgagctg
    62461 gatggtgggc agcatgattt ggcagttgcc tatgatacca ggcgcactag ctggcttgag
    62521 tcgcagagct ggaccgtgct gcgtttctgg cataacgaga ttgattgtaa tgaggaggcg
    62581 gtgctggaga ttattctgca ggaactgaac cgccggtcac cctcaccctg aaagggcgag
    62641 ggggccgacc gagccgaata gctgttgtgg tgaaaacatg gagacagtgc tggagaatat
    62701 tcggcaaggt ctgaaccatc ccagccatcg ccataaaagg gttaggggct gtatgagcct
    62761 gtttgttgct ggggtaacaa tatttgcaca atatggtccc ctcgcccctc tggggagagg
    62821 gttagggtaa ggggaaaagc gcccaccctg ccgcagcctg ctccggtcgg acctggcaac
    62881 tatagctact cacagccagg ttgattataa taaccgttta tctgttcgta ctgtttacta
    62941 aaacgacgaa tcgcctgatt ttcaggcaca acaagcatca acaataagga ttaaagctat
    63001 gggttttctt tccggtaagc gcattctggt aaccggtgtt gccagcaaac tatccatcgc
    63061 ctacggtatc gctcaggcga tgcaccgcga aggagctgaa ctggcattca cctaccagaa
    63121 cgacaaactg aaaggccgcg tagaagaatt tgccgctcaa ttgggttctg acatcgttct
    63181 gcagtgcgat gttgcagaag atgccagcat cgacaccatg ttcgctgaac tggggaaagt
    63241 ttggccgaaa tttgacggtt tcgtacactc tattggtttt gcacctggcg atcagctgga
    63301 tggtgactat gttaacgccg ttacccgtga aggcttcaaa attgcccacg acatcagctc
    63361 ctacagcttc gttgcaatgg caaaagcttg ccgctccatg ctgaatccgg gttctgccct
    63421 gctgaccctt tcctaccttg gcgctgagcg cgctatcccg aactacaacg ttatgggtct
    63481 ggcaaaagcg tctctggaag cgaacgtgcg ctatatggcg aacgcgatgg gtccggaagg
    63541 tgtgcgtgtt aacgccatct ctgctggtcc gatccgtact ctggcggcct ccggtatcaa
    63601 agacttccgc aaaatgctgg ctcattgcga agccgttacc ccgattcgcc gtaccgttac
    63661 tattgaagat gtgggtaact ccgcggcatt cctgtgctcc gatctctctg ccggtatctc
    63721 cggtgaagtg gtccacgttg acggcggttt cagcatcgct gcaatgaacg aactcgaatt
    63781 gaaataatca ttctgttggt aaatattggg cggcgttctg ccgcccgtta tctctgttat
    63841 acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct tgcgtcagga
    63901 taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca ccggcaaaag
    63961 ccactggtat catgaaacgc aatccagtac tgcggagtat gacgttctgc ctctggtccc
    64021 ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg aagaaacgct
    64081 ggctcccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggaat tgttccctga
    64141 ccagcttacc gtgacccgtt cacagacctt caccgcttat gaacgcttgt ctacggccct
    64201 gacggttgct caggtttgcg gcgtccagcg gttatgtaac tactattcgg cgcgacttac
    64261 gccgctcccc gggcctgatt cctccaggga aagcaatcat aggttggcac aaatcacgca
    64321 atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc gccagcatct
    64381 gaatgacgtc ggtcttactg ccggggactg tgtgatcatt aaccaaatca ttggttttat
    64441 tggctttcag gcgcggacca ttgcgacatt tcaggcttat ctcgggcacc cggtacgctg
    64501 gttacccggt ctggaaatac aaaactacgc cgacgcgtca ctgtttgctg atgaatcaat
    64561 acgctggcga agcagctatg aggtggaaaa actacctgaa gagtacacaa aaagttcaac
    64621 agcagaactt tgccaactgg ccgaaacact ctctctccac cctatttcac tttcccttct
    64681 cgaaaggttg ttaaacagta cacgggttaa tacacagccg gataataagc ttgcggcgtt
    64741 gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc gcctgtatgg attcatcaaa
    64801 tgaatataaa aaaatcagcc cccttctgcg caagggcgaa aatgaaatta accaatgggc
    64861 tgaccgtcat tctgttgagc acgctaccgt tcaggcgata caatggctga cccgagcacc
    64921 cgatcgcttt agcgccgccc agttcagccc tttactcgaa cacgaaaaat catcaacgca
    64981 gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct taaaaatcgc
    65041 gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac tgtcagccgc
    65101 tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta gcgcagctta
    65161 aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc acagaaaaag
    65221 gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg ccgcagatga
    65281 aaaaagtcat gcatggcgac cgaattatcg cggtgatcca cagtgaaaaa gaacgtgaat
    65341 ccgcagagcc agaagaactg gttgaaccgt tcctgactcg tttcgtgggt aaagttcagg
    65401 gcaaaaatga ccgtctggcc atcgttcctg atcatccact cttaaaagac gccattcctt
    65461 gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg cgactgggcg gttgccgaaa
    65521 tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca caatacatca
    65581 cttttggtga cgaccacttt gtaccgtggt gggttaccct tgcgcgccat aatctggaaa
    65641 aagaagcacc agacggcgtc gctaccgcaa tgctcgatga aggtctggtt cgtgaagatc
    65701 tgaccgcgct ggattttgtc accatcgaca gtgccagcac agaagatatg gatgacgccc
    65761 ttttcgctaa ggcgttgccg gatgacaaac ttcagctgat tgtggcgatt gccgatccaa
    65821 ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc gcattcacca
    65881 actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac gatctctgct
    65941 cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc tccgctgatg
    66001 gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa gcaaagctgg
    66061 tgtatgacca ggtttctgac tggctggaaa ataccggtga ctggcagcct gaaagtgaag
    66121 caattgccga acaagtccgt ttgctagcgc aaatttgcca acgccgcggc gagtggcgtc
    66181 ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc ggtgaaaaag
    66241 gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc gtcgaagaag
    66301 cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg cgataagctc ggttttggca
    66361 tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgctggca gcgttgctga
    66421 aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac gctggacggt ttctgcaaac
    66481 tgcgtcgtga actggacgcg caaccaactg gtttcctcga cagccgcatt cgtcgcttcc
    66541 agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt ctggaagcat
    66601 acgccacctg gacttcgccg atccgtaaat atggcgacat gatcaaccac cgtctgctga
    66661 aagcggttat caaaggcgaa actgcgacgc gcccacagga tgaaatcact gtccaaatgg
    66721 ccgagcgtcg ccgtctcaac cggatggcag aacgtgatgt tggtgactgg ttatacgcac
    66781 gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc agcggaaatt gtcgatatca
    66841 gccgtggcgg catgcgtgtt cgtttggttg ataacggcgc tatcgccttt attccggcac
    66901 ctttcttaca cgctatgcgc gatgaactgg tttgcagcca ggaaaacggc actgtacaaa
    66961 ttaaaggtga aacggtttac aaagtgactg acgttattga cgtcactatt gccgaagtcc
    67021 gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc acggcccatt
    67081 cctcatggat gggccgttta tttccccgct ctaccttcgt tatatccaag cagatttttt
    67141 aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg gattacaaat aaatatataa
    67201 aaaaaacaat gcgtttagcc gccgttaatt tcatgtaagt catgttgttc cttttattta
    67261 accgcggact ccgcagttaa ccggaggata tgcatcatga aaaccgttag ggagtccaca
    67321 acgttgtaca actttctcgg atcgcacaat ccatactggc ggttgacgga aagcagcgat
    67381 gttttgcgct tttctaccac cgaaaccaca gaacctgatc gtacattgca gttatctgcc
    67441 gaacaggctg ctcgcatcag ggaaatgacg gtcatcacct ccagcctgat gatgagtctg
    67501 accgtcgatg aaagcgatct ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa
    67561 tgggctggca acgcgtctgc atggcatgac acaccggcgg ttgctcgtga tttatcacac
    67621 gggctttcct ttgctgagca ggtagtttct gaagcacatt ccgcaatagt gattctcgac
    67681 agccggggga atatccaacg cttcaatcgg ttatgtgaag attacacagg gttgaaagaa
    67741 cacgacgtca ttgggcaaag cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca
    67801 tccaggcgca ataaccgtgt attttttcga agcggcaatg catatgaagt cgaactgtgg
    67861 ataccaacat gtaaaggcca gcggctgttt ctgtttcgca ataaatttgt ccacagcggc
    67921 agtggcaaaa acgagatttt tttaatctgt tccggcaccg acattaccga agagcgccgc
    67981 gctcaggagc gactgcgtat tctggcaaat accgacagta tcaccggact gccgaatcgt
    68041 aacgcaatgc aggatttaat cgatcacgct attaatcatg cagataacaa taaagttggg
    68101 gttgtgtatc ttgatttgga taatttcaaa aaggtcaacg acgcctatgg gcatttgttt
    68161 ggtgaccagt tattacgcga cgtgtcattg gctattttaa gctgtctcga acatgaccag
    68221 gtgttggcgc gtccaggtgg ggatgagttt ctggtactgg catccaacac ctcacaaagc
    68281 gcgctggaag caatggcatc acgaattttg acccgcttac ggctcccctt tcgcattggt
    68341 ttaattgaag tttataccag ctgttcagta ggtattgcac tctctcccga acatggttca
    68401 gacagcacgg ctattattcg tcacgccgac acagcaatgt acacagcgaa ggaaggcgga
    68461 cgaggacaat tttgcgtttt taccccagaa atgaatcaac gggtatttga atatctctgg
    68521 ctggatacca acttgcgtaa agcactggaa aacgatcagt tggttattca ctatcaaccg
    68581 aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg gcagtcacct
    68641 gaacgtgggt tgattccacc gttggacttc atttcctacg ccgaagagtc agggctaatt
    68701 gtgcctttag gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa gtggcgggaa
    68761 aaaggcataa acctgcgagt ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc
    68821 attttcaccg ccctgaaaca ggttctccag gaactcaatt ttgaatactg ccctatagat
    68881 gttgaactga cagagagttg tctgattgag aatgatgaac tggcactgtc tgttattcaa
    68941 caatttagcc aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg ctactcttca
    69001 ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt ttttgttcga
    69061 gatattcaca aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag
    69121 gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga tgctttttta
    69181 accaagaacg ggatcaatga gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc
    69241 gccttcgaac gctggtataa acgctatctg aagcgcgcat aagtagatga atatatatac
    69301 ttagcgctat atcccgtcat tatatatcgt attcctggat gataattagc cgttgctgtt
    69361 aaacacttct cttttagcgg caatttttgc attattcatt ttcccccttt tgctataagg
    69421 atgtccttga tgtctgaatt tgatgcccag cgcgttgccg aacgtattga tattgtgtta
    69481 gatatccttg tggcaggcga ttaccactct gcgatccata atctcgagat acttaaagca
    69541 gaactcttgc gccaggttgc tgaatctacg ccagacatcc cgaaggcacc gtgggagatt
    69601 taacaatcgt gccttcaggt tttcctattt gaggatgccc ggcgcgtcag tattttttta
    69661 tttagtatta taacgttata agaattacag cgatgaattc ccgacaacaa actattctac
    69721 agatggtcat tgaccagggt caggttagcg taaccgatct ggcaaaagcc actggagttt
    69781 ctgaagttac cattcgccag gatctcaaca ccctcgaaaa actgagttac ctccgccgtg
    69841 cacatggctt tgcagtttcg cttgatagtg atgacgtcga aacccgtatg atgagcaact
    69901 atacgctgaa gcgtgaactc gccgagtttg ccgcgtctct ggttcaaccg ggcgaaacca
    69961 tctttatcga aaatggtagc agtaatgccc tgctcgcccg gactctgggc gaacagaaga
    70021 aaaatgtcac tatcatcaca gtcagcagct acattgcgca tttgctaaaa gacgcgcctt
    70081 gtgaagtcat tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt
    70141 tgacacgcca gtgcatccag caggtacatt tcagcaaagc atttattggt attgatggct
    70201 ggcaacctga aactggattc accggtcgcg atatgatgcg taccgatgtg gtcaatgccg
    70261 tgctggaaaa agagtgcgaa gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac
    70321 attcatactc catcggtccc gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac
    70381 gcgccagcga tctgatgcat cttgagcaca gtaaactcac cgttcacgtc gttgacattt
    70441 aaccatttcc cttctctacg gatgatttgc agtttggcaa atcatccgct ctaagatgat
    70501 tcctggttga taattaagac tatttacctg ttattaacac gcttaagata taaaattatt
    70561 atcagcgata taacaggaag tcattatcac ctgcgtgata taaccctgcg cgcgagcaga
    70621 tttcacggaa taatttcacc aggcttattc ttagctatta tagttataga gagcttactt
    70681 ccgtgaatca taaattcagg agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg
    70741 gctgttctgg caattacttt ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg
    70801 gaccgcaaca ccgcaatcgg cgcgggtgca ggggcattag gcggtgcagt actgaccgat
    70861 ggcagtacgt tgggtacatt aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc
    70921 ggtaaataat cgtaaataaa tgtcgattat cgactcgcaa ttataatacg cttga
//
LOCUS       DAZFRJ010000003        69709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000003 DAZFRJ010000000
VERSION     DAZFRJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.003"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..571
                     /locus_tag="ACHKUN_000151"
     CDS             53..571
                     /locus_tag="ACHKUN_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6658481.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            587..1126
                     /locus_tag="ACHKUN_000152"
     CDS             587..1126
                     /locus_tag="ACHKUN_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO6658482.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHKUN_000153"
     CDS             complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHKUN_000153"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO6658483.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHKUN_000154"
     CDS             complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHKUN_000154"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO6658484.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4495..5865
                     /gene="xseA"
                     /locus_tag="ACHKUN_000155"
     CDS             4495..5865
                     /gene="xseA"
                     /locus_tag="ACHKUN_000155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO6658485.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5862..6077)
                     /locus_tag="ACHKUN_000156"
     CDS             complement(5862..6077)
                     /locus_tag="ACHKUN_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6658486.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHKUN_000157"
     CDS             complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHKUN_000157"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO6658487.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHKUN_000158"
     CDS             complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHKUN_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO6658488.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHKUN_000159"
     CDS             complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHKUN_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO6658489.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHKUN_000160"
     CDS             complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHKUN_000160"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO6658490.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHKUN_000161"
                     /gene_synonym="gcpE"
     CDS             complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHKUN_000161"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO6658491.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHKUN_000162"
     CDS             complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHKUN_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO6658492.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13391..14545)
                     /locus_tag="ACHKUN_000163"
     CDS             complement(13391..14545)
                     /locus_tag="ACHKUN_000163"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO6658493.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHKUN_000164"
     CDS             complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHKUN_000164"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO6658494.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHKUN_000165"
     CDS             complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHKUN_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO6658495.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHKUN_000166"
     CDS             complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHKUN_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO6658496.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNHHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22756..23601
                     /gene="sseA"
                     /locus_tag="ACHKUN_000167"
     CDS             22756..23601
                     /gene="sseA"
                     /locus_tag="ACHKUN_000167"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO6658497.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23937..24062
                     /gene="timP"
                     /locus_tag="ACHKUN_000168"
     CDS             23937..24062
                     /gene="timP"
                     /locus_tag="ACHKUN_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO6658498.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="ACHKUN_000169"
     CDS             complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="ACHKUN_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO6658499.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="ACHKUN_000170"
     CDS             complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="ACHKUN_000170"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO6658500.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26473..26673)
                     /gene="iscX"
                     /locus_tag="ACHKUN_000171"
     CDS             complement(26473..26673)
                     /gene="iscX"
                     /locus_tag="ACHKUN_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO6658501.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26685..27020)
                     /gene="fdx"
                     /locus_tag="ACHKUN_000172"
     CDS             complement(26685..27020)
                     /gene="fdx"
                     /locus_tag="ACHKUN_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO6658502.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27022..28872)
                     /gene="hscA"
                     /locus_tag="ACHKUN_000173"
     CDS             complement(27022..28872)
                     /gene="hscA"
                     /locus_tag="ACHKUN_000173"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO6658503.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28889..29404)
                     /gene="hscB"
                     /locus_tag="ACHKUN_000174"
     CDS             complement(28889..29404)
                     /gene="hscB"
                     /locus_tag="ACHKUN_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO6658504.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29500..29823)
                     /gene="iscA"
                     /locus_tag="ACHKUN_000175"
     CDS             complement(29500..29823)
                     /gene="iscA"
                     /locus_tag="ACHKUN_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO6658505.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29840..30226)
                     /gene="iscU"
                     /locus_tag="ACHKUN_000176"
     CDS             complement(29840..30226)
                     /gene="iscU"
                     /locus_tag="ACHKUN_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO6658506.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30254..31468)
                     /gene="iscS"
                     /locus_tag="ACHKUN_000177"
     CDS             complement(30254..31468)
                     /gene="iscS"
                     /locus_tag="ACHKUN_000177"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO6658507.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31709..32197)
                     /gene="iscR"
                     /locus_tag="ACHKUN_000178"
     CDS             complement(31709..32197)
                     /gene="iscR"
                     /locus_tag="ACHKUN_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO6658508.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32498..33235)
                     /gene="trmJ"
                     /locus_tag="ACHKUN_000179"
     CDS             complement(32498..33235)
                     /gene="trmJ"
                     /locus_tag="ACHKUN_000179"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO6658509.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33354..34157
                     /gene="suhB"
                     /locus_tag="ACHKUN_000180"
     CDS             33354..34157
                     /gene="suhB"
                     /locus_tag="ACHKUN_000180"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO6658510.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34302..35156
                     /locus_tag="ACHKUN_000181"
     CDS             34302..35156
                     /locus_tag="ACHKUN_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6658511.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35347..36627
                     /gene="csiE"
                     /locus_tag="ACHKUN_000182"
     CDS             35347..36627
                     /gene="csiE"
                     /locus_tag="ACHKUN_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO6658512.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36619..37758)
                     /locus_tag="ACHKUN_000183"
     CDS             complement(36619..37758)
                     /locus_tag="ACHKUN_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO6658513.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37918..38808)
                     /gene="hcaR"
                     /locus_tag="ACHKUN_000184"
     CDS             complement(37918..38808)
                     /gene="hcaR"
                     /locus_tag="ACHKUN_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO6658514.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38944..40305
                     /gene="hcaE"
                     /locus_tag="ACHKUN_000185"
     CDS             38944..40305
                     /gene="hcaE"
                     /locus_tag="ACHKUN_000185"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO6658515.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40302..40820
                     /gene="hcaF"
                     /locus_tag="ACHKUN_000186"
     CDS             40302..40820
                     /gene="hcaF"
                     /locus_tag="ACHKUN_000186"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO6658516.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40820..41140
                     /locus_tag="ACHKUN_000187"
     CDS             40820..41140
                     /locus_tag="ACHKUN_000187"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO6658517.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41137..41949
                     /gene="hcaB"
                     /locus_tag="ACHKUN_000188"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41137..41949
                     /gene="hcaB"
                     /locus_tag="ACHKUN_000188"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO6658518.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41959..43161
                     /gene="hcaD"
                     /locus_tag="ACHKUN_000189"
     CDS             41959..43161
                     /gene="hcaD"
                     /locus_tag="ACHKUN_000189"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO6658519.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43258..43680
                     /locus_tag="ACHKUN_000190"
     CDS             43258..43680
                     /locus_tag="ACHKUN_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6658520.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43728..44600)
                     /locus_tag="ACHKUN_000191"
     CDS             complement(43728..44600)
                     /locus_tag="ACHKUN_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO6658521.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44612..45706)
                     /locus_tag="ACHKUN_000192"
     CDS             complement(44612..45706)
                     /locus_tag="ACHKUN_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6658522.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45739..46737)
                     /locus_tag="ACHKUN_000193"
     CDS             complement(45739..46737)
                     /locus_tag="ACHKUN_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6658523.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46762..48273)
                     /locus_tag="ACHKUN_000194"
     CDS             complement(46762..48273)
                     /locus_tag="ACHKUN_000194"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6658524.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48296..49279)
                     /locus_tag="ACHKUN_000195"
     CDS             complement(48296..49279)
                     /locus_tag="ACHKUN_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6658525.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49376..52657)
                     /locus_tag="ACHKUN_000196"
     CDS             complement(49376..52657)
                     /locus_tag="ACHKUN_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO6658526.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52775..53968
                     /locus_tag="ACHKUN_000197"
                     /pseudo
     CDS             52775..53968
                     /locus_tag="ACHKUN_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(54032..55285)
                     /gene="glyA"
                     /locus_tag="ACHKUN_000198"
     CDS             complement(54032..55285)
                     /gene="glyA"
                     /locus_tag="ACHKUN_000198"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO6658527.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55613..56803
                     /gene="hmpA"
                     /locus_tag="ACHKUN_000199"
     CDS             55613..56803
                     /gene="hmpA"
                     /locus_tag="ACHKUN_000199"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO6658528.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56848..57186)
                     /gene="glnB"
                     /locus_tag="ACHKUN_000200"
     CDS             complement(56848..57186)
                     /gene="glnB"
                     /locus_tag="ACHKUN_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO6658529.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57247..58581)
                     /gene="glrR"
                     /locus_tag="ACHKUN_000201"
     CDS             complement(57247..58581)
                     /gene="glrR"
                     /locus_tag="ACHKUN_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO6658530.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58571..59284)
                     /gene="qseG"
                     /locus_tag="ACHKUN_000202"
     CDS             complement(58571..59284)
                     /gene="qseG"
                     /locus_tag="ACHKUN_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO6658531.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59449..60876)
                     /gene="qseE"
                     /locus_tag="ACHKUN_000203"
     CDS             complement(59449..60876)
                     /gene="qseE"
                     /locus_tag="ACHKUN_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO6658532.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61452..65339)
                     /gene="purL"
                     /locus_tag="ACHKUN_000204"
                     /gene_synonym="purI"
     CDS             complement(61452..65339)
                     /gene="purL"
                     /locus_tag="ACHKUN_000204"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO6658533.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65597..67153
                     /gene="mltF"
                     /locus_tag="ACHKUN_000205"
     CDS             65597..67153
                     /gene="mltF"
                     /locus_tag="ACHKUN_000205"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO6658534.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67150..67653)
                     /gene="tadA"
                     /locus_tag="ACHKUN_000206"
     CDS             complement(67150..67653)
                     /gene="tadA"
                     /locus_tag="ACHKUN_000206"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO6658535.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67711..68346)
                     /gene="yfhb"
                     /locus_tag="ACHKUN_000207"
     CDS             complement(67711..68346)
                     /gene="yfhb"
                     /locus_tag="ACHKUN_000207"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO6658536.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68555..69403
                     /locus_tag="ACHKUN_000208"
     CDS             68555..69403
                     /locus_tag="ACHKUN_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6658537.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69459..>69709
                     /gene="yfhL"
                     /locus_tag="ACHKUN_000209"
     CDS             69459..>69709
                     /gene="yfhL"
                     /locus_tag="ACHKUN_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO6658538.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHA"
BASE COUNT        16526 a        19240 c        17599 g        16344 t
ORIGIN      
        1 cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa tagtgatgaa
       61 atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg caggatgcca
      121 gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga ataccaaaca
      181 agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg tagacaactc
      241 ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt
      301 tatcggccac aacgtcggtt ctggcagcaa ttccggaacg acggcagggg cagttggcgg
      361 cggagctgta ggcgcggcag tgggttctat ggtgaatgat aaaaccttag tggaaggtgt
      421 ttctttaaca tataaggaag gcaccaaagt gtatacctct acccaggtgg gtaaagagtg
      481 ccagtttacg acaggtttag ccgttgttat taccacaacg tataacgaaa cgcgtattca
      541 gccaaatact aaatgtcctg aaaagagcta ataatcagga ggagtcatga agaaagtttt
      601 tctttgcgcc atcttggcct ccttaagcta tccggctatc gcctcatcat tgcaggatca
      661 actctctgct gtagcagaag ctgaacagca aggtaaaaat gaagagcaaa ggcagcatga
      721 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc
      781 ccaggccgcc gctaacaaaa gagcggcaac ggcagcggca aataagaaag ctcgtcagga
      841 taaactggac gccgaagcct ctgcggacaa aaaacgcgat caaagttatg aagatgagct
      901 acgcagctta gagattcaga aacaaaaact ggcgctggca aaagaagaag cccgcgttaa
      961 gcgagaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
     1021 gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc tgatgaaacg
     1081 cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga tgttagtaac
     1141 ttcaagccta tgattcttga agataaaaaa ccctctgtag taacagaggg ttttgttcat
     1201 tcatagtgca gggattaaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1261 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1321 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1381 cgcagagaga caacccaatc atacttacga ccatcgccca ttacgccaac ggaacgtacc
     1441 ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1501 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1561 agcacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
     1621 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1681 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1741 tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat ggtgccctgc
     1801 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1861 cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1921 cgatcttctg ccggtacgtg aacaatgtta agaccaaagt gatcgccaaa catatccaga
     1981 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     2041 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2101 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
     2161 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2221 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2281 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2341 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2401 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2461 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2521 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2581 tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg ggccgccgga aagaataatg
     2641 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2701 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2761 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2821 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2881 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2941 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     3001 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3061 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3121 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3181 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3241 ccagcatgga acctaccatc accgcgcttg cgccagctgc gatagctttg gcgatgtcgc
     3301 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3361 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3421 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     3481 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3541 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
     3601 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3661 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3721 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3781 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3841 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3901 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3961 taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
     4021 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4081 gacgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4141 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4201 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4261 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4321 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4381 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4441 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4501 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4561 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4621 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4681 ttccgcaaca gcaaccgtcg ggtgaccttc cgtccacagc atgggcaaca agttttagtt
     4741 cgcgccaata ttacgctcta cgagccgcgc ggcgactacc agataatcgt tgagagtatg
     4801 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4861 gctgaaggat tgttcgatct gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4921 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4981 cgcgatcctt ctctaccggt gatcatctac cccaccgccg ttcagggcga tgacgcgccg
     5041 gggcaaactg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     5101 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5161 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5221 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     5281 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5341 atggcgatgg attattatct cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga
     5401 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     5461 caaaagcgga tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     5521 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5581 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgtacagct tagcgccacg
     5641 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5701 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5761 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagta
     5821 aaaaacatcc agccggtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5881 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccgcag
     5941 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataatt
     6001 tccccgcagg atgggcaacg cgcatggtcg ttatcctgat caagcacatg ctgacattgt
     6061 ggacaatgca gttccatatg aattcctccg gtagccatcc ctctacattc atagagggaa
     6121 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6181 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6241 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6301 tcatcaagta gcgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     6361 caatcggcgg gttataacca ccagcgtggg catatttcag cttcacacga cgaccgcgta
     6421 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6481 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6541 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6601 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6661 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6721 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcatct tcaatggcct
     6781 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6841 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6901 tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6961 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     7021 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7081 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7141 gcagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     7201 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7261 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     7321 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7381 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7441 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7501 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7561 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7621 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7681 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7741 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7801 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7861 cgacgttaat ccagtgcaga taaccttcac tgtcacctac caccaggttg ccattataca
     7921 gcaccggaga agtcagcagg cgatgcaaca gatcgctttg tgtccacagc gtaacgccgc
     7981 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     8041 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8101 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacga
     8161 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     8221 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8281 cgccacccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8341 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcctcg ttcagcgctt
     8401 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     8461 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8521 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc agaccgccag
     8581 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     8641 tttctttgcc atcatccgca ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga
     8701 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8761 cggaagtgct ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8821 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8881 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8941 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     9001 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     9061 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9121 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9181 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9241 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9301 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     9361 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9421 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9481 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     9541 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9601 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9661 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9721 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9781 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9841 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9901 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt
     9961 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
    10021 tcacccactc gaaaacggta cggttatagt aatccagacc acgcaccaga cgctggttta
    10081 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10141 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10201 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10261 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10321 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
    10381 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10441 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10501 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10561 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10621 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10681 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10741 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10801 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10861 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcta tgcgggttca atcatacacg
    10921 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10981 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgct tccagctggt cgatcatatc
    11041 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc
    11101 gccggtgacg ccgagtgtag aaaccagcgc ctcacctggg ccattcacca cgcagccgat
    11161 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt
    11221 accgataaca tcaaattcct gacgcgaaca ggtcgggcag gcgatgaagt tgatcccgcg
    11281 cgaacggata cgcagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11341 ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    11401 aatggcggat tttactgccc cgctgcgtgc accaccggct tcggtgatcc ccagatgcag
    11461 cggctgatcg atctgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11521 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatggcg
    11581 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11641 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11701 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11761 gacgccgtat tccgctactt tcagtgcaat gcgatagtcg aagtggatgt cagccaccag
    11821 cggcacgtta acctgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11881 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11941 ttcgacgtct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    12001 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12061 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12121 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12181 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtatgg tgcctggccg
    12241 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    12301 tcagtaacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12361 gccggcgtag tcacgcctgc ctgatcggtt ggcaacggcg cagcgccatc tggcgttgtt
    12421 gttgccgttg gtgccggggt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    12481 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    12541 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12601 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12661 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12721 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtca
    12781 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatag gcgcaacttt tgcagcccgt
    12841 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12901 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12961 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    13021 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13081 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13141 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13201 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13261 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13321 attgcaactt aatacttcat tcataaaaga ttacgccacg gtacataaag taaccgtggc
    13381 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    13441 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13501 caccacgggt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13561 ggtcgatacg gctgttcgag ctgcgtccat acggcgcgcc cgggaagggg ttccacggga
    13621 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13681 tgccgtcgtt aacgtggtca agcatcacat actcaatagt gacacggccc tgattggcgt
    13741 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13801 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg
    13861 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtagaaagcg
    13921 tgacgcgacg tttagacagg ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13981 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    14041 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccaa
    14101 taatttccga tacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14161 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga
    14221 tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg
    14281 atgaacgctg ttcttcaacc acttccggtg cgcggatttc cgccacctct ttcagtttgc
    14341 cgcgcaacac tttgttgatg tcggtcatct catcaaagtt gtcgcagcaa tagtgataca
    14401 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14461 gcatctgctg acggttgaga tccagcaggt tgatttttcc atctttcgtg gtgacgtttt
    14521 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14581 cctggaggat tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14641 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14701 ggtgcgcggg cacacttcgc cttcgccaaa gaaataagcg atttcgcgag cggcagattc
    14761 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14821 agtaccagcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14881 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14941 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgtgcct gttcaacggt
    15001 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15061 attaccaatg acgttttttg ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat
    15121 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    15181 ggattataat gagcaacagg gccgttgact attgatgaag gtaacatttt tttaaaataa
    15241 aatgagtttt agcaacaatc agggacaaaa cagactattg catgacaaat ttcactgtcg
    15301 cgatttgccc cacatcatcc atcaccagca attgataatc acctttatcc gtcaaatgca
    15361 gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt cagaaaccac cagcgttcac
    15421 ctgcccctcc acttgattgc aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa
    15481 taatcgcgcc atcgcgaacg cctgtcagct gcagagggag ctgggcatcg tgaccgtatg
    15541 acgggcaaat tgtcgaggcc ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc
    15601 agggttccag cggtagcggc cagacattta tcatttcttg tcgcgcttgc gggcaatcag
    15661 cggcaacacg tttgccattt tcatccagcc agatggggaa acgaatgcca ttaatgcctt
    15721 cctgctccgg cagtaataga gttggcggct gactcccgtc cagcagccag gttgccaggc
    15781 ggcggcgaca gttaccgtca ccttccggca aagactgtcc gcccggccag cagataacgc
    15841 cacgagtgac tgagttcggg cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga
    15901 tattattgac ctgattcaac aatggtacgg cactggcaaa gccaaactga ccaacaacgg
    15961 gcgtgccgtc cggtctgcca gtccagatcc caatgacata gcgagcgtta accccaatcg
    16021 cccaggcgtc acgatagcca tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg
    16081 gcaaggcact atccggcaag ggtttcgctt catcagccat aatccggcga atgatccacg
    16141 ccgcccccga cgacattaaa ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt
    16201 tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc cgccatatct tccagttttg
    16261 caccagcgcc gccgagaatg agtgaaagat tcggcgcagc accgttgggc aaatataacg
    16321 gcaatccaac attgcgtaac tttgccgcaa accgtttcgg tccataggct tccagcacct
    16381 gcacagcagg taagttcagc gagcgcacca gcgcctcgct catgctgatc gggccatgaa
    16441 aaccgctatc aaagttacct ggtcgataat caccggtgcg ccgggggacg tcttgcagca
    16501 gtgatgccgg gtggatcaag ccttcatcca gcgccagacc ataaacaaac ggtttgagca
    16561 ccgatcctgg cgatcggttc gcattgacca tatcaacatg accaaagcgt gaatcatcgt
    16621 tgagatcaac cgatcccacc cagccgcgaa cacgcatatc ggtatgatca accacgatca
    16681 tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac
    16741 gtcgttgaag accggcatcc agcgtagtaa cgattttgtc gcttttgctt ttaccgagca
    16801 tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg gggtgccagc cagatgggtt
    16861 cttcccttga ctcttttacc tgctcacggg accacgcacc ttgcacggcc atccgttcga
    16921 gcactttatt acgcgcggct tcggcacgct ccggccaacg atccgggcga agacggctgg
    16981 gcgcttgcgg caaaaccgcc agcattgccg cctcggaata gcttaaattc gcaggcgact
    17041 ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc
    17101 ggttaagata caaggtcaga atttcacgct tagacagatg ccattccagt tgcaacgcgc
    17161 gccagagctg gcgaattttg ccgccaaatg ttttggggtg aggatcaagc agacgagcaa
    17221 cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc cgaagtgaga tcttgccatg
    17281 ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt ccagaaccag cgatcttcat
    17341 aattgatcag cgcttcaagg taacgtggag aaacatcttc gattgttacc ggataacgcc
    17401 agatgccgtc agcatcggcg aagcgccata gcggcgtacc atcctgcgcc acgaccactc
    17461 gtgcgggatt gacttcatgc agcggtagag gccagagttt atctgctccc cacgctgcaa
    17521 gaaaaaggag aaatggcgca gctgccagcg ttatccagca gccgcgtttg gttaacaagc
    17581 gaggcattta cggtcggaca atcagcagat cttcagccgc gccggtcgcc cgccattggg
    17641 gaacatacat tgattccacc atcggttgcg gtacctgata cgttcccggc gtcaccgccc
    17701 gcgccagata caccagcgtt accggttggt attcatcaac ggcaaccgcc gccacaaagc
    17761 gatcgtcacg gaactcaatg tgcttaatac tcgcctgctg catctggttc agtaagttct
    17821 gcacttcgcc accactttgc tccaggctgg cgctaccgtt cgccagattc tggttttcca
    17881 gttccagacc cgcaggcagc agatccacga ccaacgcatc cggcacgctg ttactggctt
    17941 ttacctgcaa ccacaccagc accagatcgc cgctacgtaa cgagtccagc gatttgctct
    18001 taccatcagt accaagaata tgacgctcga tttgcagcac atcgttcgcc ggtaaaggtg
    18061 cggattgcgg ataaccgctg gcatccacac gcagccataa cggctgatcg ccactgtttc
    18121 tcacctgcaa ggtggcaagt tgatcgctat tcagattgct gtttagcgtt ttctcgcctg
    18181 tcagcggctc agctgagaaa gaggtttgcg cctgccattt acccggtaaa tcctgaatcg
    18241 tacgggcagc caggaacaac gcgttacttt cctgcgtcga tagccagcgt tcaccaaatg
    18301 cctgctgcga aagcgtgttc agcaaagtgt tctgctcatc gggtagcagt ttattttctt
    18361 ccagcaagga gagcattaac gcgttgtcgc gcagtggact accgtaatca cccagccata
    18421 tccgctcatc actatttctc ggcgttttca gcgccagcac aatcgcttct tcaccacgcg
    18481 ttgcatcacc catggttttc agcgcaacgc caagttgcag cagcggtaaa ccagaagcgg
    18541 catctgcacg atgctcccag atttcacgca gcgcacccag tggtgctttt tgctgacggg
    18601 ccagcaccag cgcagcgtaa gactgaacgg cgaatttact ggctttgaga ttatccgcgt
    18661 acgggatcga catcatgccc ggatcttgta aatagcgcag cagacgctca ttaccccggt
    18721 taatggcgtc tgtcggcacg ctgtaaccct gttcgcctgc gcggaccagg aaatccatca
    18781 cgtaagccgt cagccagtac tcttcgtcac cgtttttatc ccacagcgca aagccgccgt
    18841 tatcacgttg catttgcagc aaacgggaaa tgccgatatc gaccgatgca cggcgtttct
    18901 catcactgtc gcctttgatg cccaacgcct gcagttgggc tgcgttggta taaagtgacg
    18961 ggaacaggcc gctggcggtt tgctcaagac agccgtacgg atacgctttt aactctttga
    19021 tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa caattgccct tccagcgtaa
    19081 caggcgagaa gttttgcaat ccatccgccg gaatcgccca tgtctcacca ggctgtaacg
    19141 ccgtaccgta attaaccgtt tgtgccggga acgccggacg gacgccgatt ttccactgct
    19201 tatgctgatc ggcaacggtt tcacccggta acgctaaccc gctaatggtg gcctgaattt
    19261 ctccatcgcc ataacccggc aatgctcgca ccgggataaa cagcgtagta cgcacacctg
    19321 gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca
    19381 gggcaacgtt cagtttttgc ggtttatcgg taagattagt gatatccagc gtcagacgcg
    19441 aggtatcgcc actcgccata aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa
    19501 ctatcacttt gctttcgttg ctaccgaaat catctgccgt ccaggcttgc gccatgacgc
    19561 gcaattcacc gttaaaatcg ccaatcggca gtgtaaccga gccttcgccc tgttcgttga
    19621 gcgttaccgg cagcgcctgc tgcgcgacaa tattgacgtg attgaccggc ggtttaccac
    19681 cacgtttcag ctcatcacca tcgccaccga aacgcagagc tgccagacgc ccctgacctt
    19741 caataacctg accgtaaata tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg
    19801 cctgccacgg atctggcgtg acgtagtcag taatattcag cacaccacta tcaacggcag
    19861 acaccagcac attcacctgt ttaggcttct cgccattttt agtgctggct ttaattttca
    19921 cggttaatgg ttgattggga cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac
    19981 ggttttcatc gccaagcggc agatgcaaca caccaaccgc gcgttttggc gtcgcggagc
    20041 gagatttatc gccaggacgt accaccagcg tacttaaata cagatcatga tgattccagg
    20101 ttttatcgac cggaatcgtc agatccagcc cttgagcccg aacatcaatc tcttgccacc
    20161 acagcggccc ttcactggac tcgaccatcg cataaccttt acccgccgtt ggcgcggcga
    20221 tatgcaactt aatggtgtcg ccagggcgat aactggcttt atccagtttc agcgtgacac
    20281 ggtcgggtcg cactgcgccg ctaccgtcgc tgttgtcctg ccagctatag ccagcccaga
    20341 aacgaacact actgaccgct tcattcggcg ctttgacttc cagacgataa gcgccccact
    20401 ctaccggaaa actgaccttg ccggtttcgt ccgctttcag atccagagtt tgttcatttt
    20461 cgatcagatc tttttgatca aactgtgact gccagccttc atcttctgac cagttccagt
    20521 agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc cgacacggct tttttcacgc
    20581 cttgcgcatc gctataaacg atgtcaaaag cggcgttact gccttcatca acaatgggct
    20641 gtttcaccgt gctgtcagta cgataatcgt aaaccgattt cgaggcgaac tgcggacgga
    20701 tccccggcaa tgcatcggca ggccagatag cctgctcagc gcggcgcgtc accgggcgac
    20761 cgcccgattc cagcaaacta ccctggaaaa taacctgtaa tggggaatgc gtttccttcc
    20821 actggctttc tgtagaaact tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat
    20881 ccagcgtgcg ggaaagattt tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg
    20941 acacagcttc acgcagtggg cgcaggaaaa gttgcccttg caaagtatta ccattagcag
    21001 gtgcaccata caggtagtac cccaccacgg agaatttcac ttcatctttc ggcgttagcg
    21061 gggttttctc accggtcaga ttcagcgcca tgcgctctgg cataaaatct tcgacgtgga
    21121 aatcccacat ccgatactgg ttatcgcccg tgttagcgcg aatatgccac ataccggttg
    21181 ccgcattgct atcgagtggc caggtaaagt ggtagaggcc attctccggc tgactaacga
    21241 cgctcctgag tacctgccca tcgggtttaa tcacgtctaa cttgatgggt tgattgggca
    21301 acgctttacc gtctgcatca cgcagcaaac cattgaggat taccgtttca cttgggcgat
    21361 aaagatcgcg tgggccaaac atgaaaaact gtttgctata gcctggcgcg ccagcaatgt
    21421 taaattctgc taagtccagc gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt
    21481 tacgcgccaa cagtaatgcc gcgtttttat cattttccag ctgcacatgc ccctgagcgt
    21541 cactggttgc ctgagtcaga gtctgccctt tctcatttaa taaagagact tcaattcctt
    21601 gctgggccgc gccgttttcc agactttggg taaagatatc cagacgattg tgataacggt
    21661 gagctgaaac gccgatatca cttaacgtaa acagcgtcgc gggattactg taatcgtaac
    21721 gtccagcctg attcatcaca gccagataca cgcccgcctg ctgaagcggt ttgatatcgc
    21781 ccagcggcag caataatttt tcacgggtgt tacgcgcagg attgagatca aaccgtccgg
    21841 tgtagaccag atccgccatc tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc
    21901 ggtattccca ttggctaatg aatgctggca gagattctgg cttaacgcgg aagaagttaa
    21961 catcgacatt attaacgttg agcgccatta ccggcagccc ttcaacgact ttgccaggca
    22021 gcagcgaacc acggctggca aaaccgacgc tgggttggat gtcgcgggta gttatagttt
    22081 tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa
    22141 cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtaa ctctttaaga ttatctgaca
    22201 gctcccaggc accatccact ttgccgcttt ttttatcgac gacatgaata acgcgtgaga
    22261 aatcctgatc cgggtcgaga gggatggaga acgtcagcac cagcgtggct gcaccatcaa
    22321 gttggacttc agagagatcc agcaatgtca gcaccttacc ggcactctgt tgggccagtt
    22381 tttgtctctc cggcacggag agttttgctg aactcgcgtt ttctgaagag gccgctttag
    22441 taacttcaga aggcgcatct tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc
    22501 ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct
    22561 gccacaatgg cccgttagca acgtcgaata attattatgc gtgagaattt acgttatgta
    22621 attcagtatc acgcccatac aatccgaatt ttagtgagaa ttgtggctca gacagccaaa
    22681 tcagcctctt tcgcttgtcg cttttcacta aacatccgac aatttagcct aaccccggca
    22741 aaaatggaga tgcctatgtc cacgacatgg tttgtaggag ccgactggct cgccgaacat
    22801 attgatgacc cggaaattca gattattgat gcccgcatgg cgtcgcctgg acaggaagat
    22861 cgtaacgttg ctcaggagta tctgaatgga catattcccg gcgcagtgtt ttttgatatc
    22921 gaagcgcttt ctgatcacac ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc
    22981 gccgtggcga tgcgtgaatt aggcgttaac caggataagc acctgattgt ctatgacgaa
    23041 ggtaatcttt tctcagcccc acgagcatgg tggatgctgc gcacctttgg tgtagagaaa
    23101 gtgtcgattc tggggggtgg acttgcaggc tggcagcgcg atgatctgct gttagaagaa
    23161 ggtgcagtag agctgccgga aggagagttt aacgccgcgt ttaatcctga agccgtggtg
    23221 aaagtaaccg atgtattatt ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc
    23281 ccggctgcac gttttaacgc agaagttgat gaacctcgcc caggtttacg tcgcggacat
    23341 attcccggag cactgaatgt tccgtggacg gaactggtgc gcgaaggcga actaaaaacg
    23401 accgatgaac tggatgcgat attttttggt cgcggcgtca gctacgacaa accaattatc
    23461 gtcagctgcg gctctggtgt aacggcagcc gtggttttgt tagcactcgc gacgctggat
    23521 gtgccaaacg tgaaactgta cgacggcgca tggagtgaat ggggcgcgcg ggcagattta
    23581 ccggttgagc cagtgaaata agtattttac aggcaataaa aaaccgccga atttggcggt
    23641 tttttattgc tggtctggtt cgcggccttt ccagcaggtt gtattaccgt agtaatgcaa
    23701 gcgcgtctca gcggagacaa tactcgccag taactctctt tttgtcaagc aaaagagagt
    23761 tattattgtt ctgttagtgt attatccact gcggcccttt ccgccgtctc gcaagcgggc
    23821 gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa gcgcgtctca gcgcttgcta
    23881 tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga
    23941 agatacggtg cttctgtatc gtacttattg tttctggtgc gctgttttcc caagtaaata
    24001 ataaccggag tctctccggc gacaatttac tggtggttaa caaccttcag agcagcaagt
    24061 aagcccgaat gccgcccttt agggcggcat attttagatt atccggttct gtttaaagtc
    24121 acgcaaaaaa ccaccccagc gacgttcata gaatggcgca atatgttcgg taataaagtg
    24181 gctaattcct ttttcccctt ttttcacctg acaaatatcg attggttcat cgccaggtaa
    24241 tgtatcggtc gctacacttc ccgtcgcctg aataatttct tcgatatcac catcggcttc
    24301 aatgccaata agtaaattag gctgtgcctc ttcgttctct ttaattgaac aaataaaagc
    24361 acgcttcacc ggcttaatgg ttttaaataa ggtggtgagt gaatcaatca tttgtgctgg
    24421 cggctctgcg acttccgata atatcagcga ttcaccgcct tccaggattt cctggctgct
    24481 cagcggattt ccctctccac caatcaacaa actgatttca cgcggcataa attctttacc
    24541 ggtcggcagt ttggcattaa ggaagagcgt ttcgccaagg gtcatctcaa acaatgtgcg
    24601 aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc tgttgaagcg cttctaacga
    24661 ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc cagtgctgta aatcaagcgc
    24721 actatcttca accacagcct cgccctgcgc cgccgtacca ggcacccaga cggtggattc
    24781 cagtagagta cggaaaaagc ccgggcggtg cgccggttcg gttgctgctt tttccagcag
    24841 gtcttcaagt tcgttttttg tttcggacat atgaaccaca attcattcaa cgttcggggc
    24901 gcaaatgcct gatgcgctac gcttatcagg cctacaaggg attcgcaatt tgttgaattt
    24961 gcagaatttg taggccggat aaggcgttta caccgcatcc ggcatattag tttacgccgt
    25021 taacagatta gctatcgtgc gcacaccaag tcccgtagcg cccgcagacc actgttcaac
    25081 cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc cagccttgct gatagttctc
    25141 aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct gccggatacg ccgcgcttcc
    25201 ggtattgttc agttcggcaa agttagacgg cagctggctg cggtggaatt ccgccagcgg
    25261 cagacgccag aatggttcgt tctcttgtga ggcactcgcc agcaaacgac cggcaagcgc
    25321 atcgtcaaaa ctgaacagcg cgtgataatc attacccagc gcagttttcg ccgccccggt
    25381 gagggtcgcc gcatcaatga tcatttccgg tttctgcgca ctggcatcaa tcagaccatc
    25441 ggcaagcacc agacgccctt cggcatcagt gttcatcact tcaacttttt taccgttgcg
    25501 ataggtgatg atatcgccca gcttgaacgc attgccgcta atcaggttat ccgcacagca
    25561 gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg gcaaatgcca gcgccccggt
    25621 aaccgttgcc gcgccgccca tgtccgactt catcgagtcc ataaacgcag tctgtttgat
    25681 gctgtagccg ccggagtcaa aagtgatacc tttacctacc aggcacgcgt acaccggtgc
    25741 ttctttatcg ccagttgggt tgtaatccag cgccagcaat accggagaac gttctgaacc
    25801 gcgcccgacg gtgtgcagcc ccatataacc ttgctcacgc agatcttcgc ctttggtgat
    25861 ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga tcaacagcac gctgtgccag
    25921 ttgcgatggt cccaattctt ctgccggtgc gttgatggtg tcacgcaccc agtcgatgat
    25981 catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc agatccggcc actccacttt
    26041 acgcgtgcct ttcggggctt tgtaaccttg ccagaatgcc cagcagcgat ccgcatccca
    26101 gccttcaccg cttaactgaa catgcttgat gcccagaccg tcaatcttgc gcgccgcacg
    26161 ctggatcagc cccagatcgt ctgccccgtt caaatgcagg gtaatgccgt cattattaat
    26221 gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt
    26281 catcgcttct gtcattttag ttatccttct ttgtaaaagg gccgccagtt ggcagcccca
    26341 ggatttttcc gctaaatgat tcgcgttgca ggaaggcggc aagtgagtga agccccagga
    26401 gcatagataa ctatgtgact ggggtgaacg agcgcagcca acgcatctgc ggcgtgaagc
    26461 atgacgcgga aattattcgg cctcgtccag ccagactaac aaaatcgctt cgaggatttt
    26521 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
    26581 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
    26641 aatttcgcgg ctatcggtcc atttaagtcc catactaacc tctgttaatg ctcacgcgca
    26701 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
    26761 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
    26821 tctgagcttt ccggcagcga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
    26881 gcacaggatt tttcacaggc gtgttcaatc tcgataccgt tacgcagagc tgcgtcgaga
    26941 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagagatc ctgatgaggc
    27001 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
    27061 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
    27121 cgcttgttcg atggcgtcaa cgtcatcgcc ctgcgccact tcactcaggt gagcggcagc
    27181 atcgtcaatg acctgacgtt ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
    27241 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca ttcgggcttt
    27301 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
    27361 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
    27421 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
    27481 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
    27541 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
    27601 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
    27661 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca acagcatttc
    27721 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
    27781 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
    27841 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
    27901 tacacccgcg tctttaagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
    27961 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
    28021 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gcggcatcca gcagttcacg
    28081 ctgaacgcgg ttatcgctac gatcaggaat gcccgcctgc tcgcgaatgt aatccgccag
    28141 cagatggtcg aaatcatcac caccgagcgc ggaatcaccg ccggtcgcca gcacttcaaa
    28201 cacaccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccgccga ggtcataaac
    28261 ggcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cggtcggttc
    28321 gttaagtaag cgcaggacgt gaaggcccgc cagacgcgcc gcgtctttgg tgccctgacg
    28381 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
    28441 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
    28501 cagcagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
    28561 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
    28621 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
    28681 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
    28741 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
    28801 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
    28861 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
    28921 cggcactgct ccgcagttta tcgagaaaac gcagcttacg cacggtatcc gccgccgcgt
    28981 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
    29041 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
    29101 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
    29161 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
    29221 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
    29281 cctggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
    29341 ggctcagcgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
    29401 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
    29461 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
    29521 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
    29581 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
    29641 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
    29701 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
    29761 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
    29821 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
    29881 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
    29941 cttcagcaat atcggtattt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
    30001 attcggtgac cagggagctg gaagcgatag cagaaccgca gccgtaagtt ttaaaacgcg
    30061 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcatcacg tcgccacagg
    30121 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttgtcaaag gaacccacgt
    30181 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
    30241 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
    30301 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
    30361 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
    30421 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
    30481 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
    30541 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
    30601 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
    30661 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcctcg cccattccga
    30721 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
    30781 tttgcgcttc gatgcgtacg cgcggtttac gacgtacata cagcgcaccg atacctttcg
    30841 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
    30901 taggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
    30961 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
    31021 tggaaacaag gatggtgtcg tcacgcatcg ctgcttcaag ttctttcagg tcgataatgc
    31081 cgttacgctg cggtgccagg taggtgactt caaaaccttc gcgctccagc tgacggcagg
    31141 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
    31201 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
    31261 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
    31321 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
    31381 ccatcgtcat aaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
    31441 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
    31501 aggaaccagg ctaccggctg gatgtacgac cgtgtttacg gagcgcgata cgccccggcc
    31561 tgattctaat atcttgttat ctttaaccta actgtttcgg gtttcagcaa gttgacaaac
    31621 ttacgaatct ccgggagctt acataagtaa gtgaccggag tgagtatgtg cagtcaacgc
    31681 agatgcaaca cgaaacagga cggttaaatt aagcgcgtaa cttaacgtcg atcgcgtctt
    31741 gtatgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct
    31801 ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca
    31861 aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct
    31921 gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc
    31981 tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac
    32041 catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt
    32101 cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg
    32161 cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc
    32221 ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac
    32281 attcccgagt aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca
    32341 gtgccatttt tttatcgtgg cgtttgtacc tgttgtcctt ccagaccatg ggcgggttga
    32401 gggcaattat tcgcattagc gctggcctct tcccctcacc ctaaccctct ccccgaaggg
    32461 gcgaggggac cgtccgtgca tttatctggc ttagccctca ggccttatta cctttattct
    32521 gctgctcaat agaagccaga atcccgcgca ggatattcaa ttcctggctt tccgggcgcg
    32581 cacgggtaaa cagacggcgc aatttattca tcacctgccc cggatggttt tcacggataa
    32641 aaccggttgc cagcagcgtt tgttccagat gaccataaaa acgctccaga tcatcgacca
    32701 gcggatacgg cgtctcttca tgctcgacct gctcgccgtt ttcctgagtt gccagccagg
    32761 ccatgcgcac ttcataggca ataacctgag ccgccatcgc caggttcagc gagctgtatt
    32821 ccgggttagc cgcaatcgcg acatgataat ggcatttctg caactcttca ttggtcaagc
    32881 cgacgcgctc gcgaccaaac accagcgcca ccggggtatt tgccgcttca gcgacgcttt
    32941 tcaggccgca ttcgcgcggg tcgagcatcg gccatggcag cgtgcgggag cgtgcgctgg
    33001 tgcccaccac caggctacaa ccggctaacg cttcgtcgag cgtatcgacg atgtgagcat
    33061 taccaatcac atcgctggcc cctgctgcca gggcaatcgc ctgggagtcg ggtttcacca
    33121 gtggattaac cagccacaga ttggttaatc ccattgtttt catggcacgg gcaacagaac
    33181 ccatattgcc ggtgtgtgac gtctccacca gcacaattcg aatattttgc agcattgtct
    33241 ttcttcatct aaagattatt cacgcatctt atcataaaac gaagacagat gccgatctcg
    33301 ctgctatact ctgcgccgtt ttcccgttct ttaacatcca gtgagagaga ccgatgcatc
    33361 cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg taatttaatt gccaaaaact
    33421 atgaaacccc ggacgctgta gaagcgagcc agaaaggcag taacgatttc gtgaccaacg
    33481 tagataaagc tgccgaagcg gtgattatcg acacgattcg taaatcttac ccacagcaca
    33541 ccatcatcac cgaagaaagc ggtgaacttg aaggtactga tcaggatgtt caatgggtta
    33601 tcgatccact ggatggtact accaacttta tcaaacgtct gccgcacttc gcggtatcta
    33661 tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt atacgatcct atgcgtaacg
    33721 aactgttcac cgccactcgc ggtcagggcg cacagctgaa cggctaccgt ctgcgcggta
    33781 gcaccgctcg cgatctcgac ggtaccattc tggcgaccgg cttcccgttc aaagcaaaac
    33841 agtacgccac tacctacatc aacatcgtcg gcaaactgtt caacgaatgt gcagacttcc
    33901 gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc tgcgggtcgt gttgacggtt
    33961 tctttgaaat cggtctgcgt ccgtgggatt tcgcggcagg cgagctgctg gttcgtgaag
    34021 cgggcggcat cgtcagcgac ttcaccggtg gtcataacta tatgctgacc ggtaacatcg
    34081 ttgctggtaa cccacgtgtt gtgaaagcca tgctggcgaa catgcgtgac gagttaagcg
    34141 acgctctgaa gcgttaatga ctcaggcggg tgatgtcact cacccgccct cgcctttcag
    34201 gcgctattct gaaatacttc ctcaccgctt tactttcttt cgtcactctc ccaccatctt
    34261 tccccgatta atggatgata aagaagtcgc aaggaaaggt tatggcactg ccagtgaaca
    34321 aacgcgttcc caaaattctg tttattctct ttgttgttgc cttctgcgtt tatttagtgc
    34381 cgcgcgttgc catcaacttc ttctattatc ctgacgataa aatttacggt cccgacccct
    34441 ggtcggcgga atccgtcgaa tttacggcta aggacggtac tcgtctgcaa ggctggttta
    34501 ttccttcttc gacgggccct gctgacaacg ccatcgcaac catcattcat gctcacggca
    34561 atgccggaaa tatgtccgcc cactggccgc tggtcagttg gttacccgag cgtaatttca
    34621 acgtttttat gtttgattat cgcgggtttg gtaaatcaaa aggcacgccg tcccaggccg
    34681 gattgctgga cgatacgcaa agcgccatca atgtggtgcg ccatcgcagt gatgtaaacc
    34741 cacaacggct ggtgctgttc gggcagagta ttggcggggc gaatattctt gctgttattg
    34801 gtcagggtga tcgtgaaggc atacgtgcgg tgatcctcga ctccacattt gcctcttatg
    34861 caaccatcgc caaccagatg atccccggca gtggctactt acttgatgag agttacagcg
    34921 gcgaaaatta tatcgccagc gtcagcccga tcccgctttt actcattcac ggtaaagctg
    34981 atcacgttat cccatggcag cacagcgaaa agttgtatag cctggcaaaa gagccaaaac
    35041 ggttgatcct aatcccggat ggcgaacaca ttgatgcttt ttccgatcgc cacggcgatg
    35101 tttatcgcga acagatggtg gactttatcc ttagcgcgtt gaatccgcag aactaaccca
    35161 tgatcgctag cacgataatc attcacaaaa ccaccttaag acatgctaat ccactggtca
    35221 gaacagttta agatgagaaa aattctgtga cgcttgccaa catttctgat gattagcatt
    35281 cccttcgcca tttccctgag caaactttag ctattcttat caattatgct tatgggagat
    35341 ctacagatga tgcctacgct tgctccacca tctgtccttt cggctcccca gcgccgctgc
    35401 cagatcttgc tgacgctctt tcagccgggg ttgaccgcca ccatggcaac cttcagcgag
    35461 cttaatggtg tggatgatga tattgccagt cttgatatca gcgaaacagg acgggagatc
    35521 ctgcgctatc atcaactcac actgacgact ggttatgacg gtagctaccg ggttgaaggt
    35581 acagtgctta accaacgttt gtgtttattt cactggctac gacgtggttt ccgtctgtgt
    35641 ccgtcattta ttaccagcca attcaccccc gccctgaaga gtgaactgaa gcggcgcgga
    35701 attgcgcgta acttttacga cgataccaat ctacaagcgt tagtgaatct ctgctcccga
    35761 cggctgcaaa aacgctttga atcgcgcgat attcatttcc tgtgtctgta tctgcaatat
    35821 tgtttgctgc agcaccacgc tggaattacg cctcagttta atccgctcca acgtcgctgg
    35881 gcggaatcct gccttgaatt tcaggtagcg caggaaattg gacgccactg gcagcgtcgg
    35941 gcgctccagc ctgtaccgcc tgatgagcca ctgtttatgg cactactttt ttccatgttg
    36001 cgggttcccg atccactgcg ggatgcgcat cagcgagaca gacaattgcg tcagtctatc
    36061 aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc gtttttatga tgaacagggg
    36121 ttatgtgatc agctttatac ccacctcgcc caggcgttaa atcgcagttt gtttgccatc
    36181 ggtattgata ataccctgcc ggaagagttc gccagactgt acccacgcct ggtgcgcacc
    36241 acccgcgcgg cgctggccgg atttgaaagt gaatacggcg tccatctttc tgatgaggaa
    36301 agtggtctgg tcgcggtgat tttcggtgcc tggctaatgc aggaaaacga tctgcatgaa
    36361 aaacagatta ttctactgac cgggaatgat agcgagcgag aagcgcagat tgagcagcag
    36421 ttacgcgaac taacgttact gccgctcaac attaagcata tgtcggtaaa ggcatttttg
    36481 cagacaggcg ctccgcgtgg cgcggcactg attattgcgc cttataccat gccattaccg
    36541 ctcttttcac caccgctgat ctatacggac ctgacgctga caacacatca acaggagcag
    36601 atccgcaaaa tgctcgaatc agcatgaggg aacaactttc gggcgcaaaa acattgccgg
    36661 cagcgccacc agcgccatta cccagaacac gccgtggccc agatattgat acaggaaacc
    36721 ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg acggcagagt aaaccgcctg
    36781 taaacggatg acttcgctac cctggcgagc agcaatataa cgcatggcgg ccaggtggca
    36841 gaccgtgaag gtgccgcaat gcagaatttg caccactatc aaccacggca acgccgtagt
    36901 tgctcccata atgccccagc gcactacgcc gcaaatcgcc gagatcaaca gcatatcgcg
    36961 tgcactacaa cggcggaaaa gtttattact cagcgcaaag ataatgactt ccgccaccac
    37021 gcccagcgac cacaaatacc ccaccgccga ggccgagtag ccagctgcct gccagtaaat
    37081 ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat aaacaaacgc aggccagaaa
    37141 gcgccagttc tggcgaacca gcgccaacca cgcagaccaa ccggtgctct cctgctggcg
    37201 acttgcccct tgtggctgaa tcgtcggacg gatgagaaag ccgagcagca tggatgccac
    37261 gcccaacgtc aacagcgcga ggatcacccg ataatcaaac atagtgacca gtttgcccgt
    37321 cagcgccgag ccaatgacaa acgccaccga gccccacagt cgcactttgc cgtaatcaag
    37381 cgggaactgc ttttgccacg tattcgccag tgcatcggtc aacggtacca gtggtgagaa
    37441 aaagaggtta aagccaatca tcaccagcat cagccacgct acgtgcgccc ccgcccagaa
    37501 ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc aaggcggaaa tcaggcggga
    37561 aggatcgctg acgcggggcg cgatgagcaa actcccgagg aaacgggcaa ccagacctgc
    37621 ccccaataac aggccgatgg tttctggcgt taaaccaatc cctttaagcc agacgctcca
    37681 gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa tagccgagcg ccaaccagcg
    37741 cgtggattgc aaaaccatga gtccctcccg tcaggaggcg ttagtctggg gttaatcgaa
    37801 tgttggtgca agttgaaaaa taagaatatt cgagcctgct tcccgatgtc tgtaacgcca
    37861 gttcacaagc actaaaaaac tgagatccgc gacgcatgtt gcaaaaataa tgcgtaatta
    37921 tgccgttacg cttgccaaac gttcctgcac aatggcgatg aaatcgcgta atgccggctt
    37981 catctcccct ttcttccacg ccatcagtaa agcaatggaa ggcacatttc cggcaatggg
    38041 acgaaaaaca acctgtccgg tattaaaatt attcatataa ccgggtatca aagtgacgcc
    38101 cagccccatg cccaccagat tcatggtcac cagaatattc gttgccacct ggacgatatt
    38161 tggctggctg ttttcttgcg caaaccacgc tttaacgatc ggcgcaagcg aaccggaata
    38221 caccggatcg gtactgacga aattcacgcc atccagttgc gcggcggtga tctctttttc
    38281 atgtgctaaa gggtgatcaa ccggtaacac aaccactaat ggttcgtcaa aaagctccag
    38341 ataatcaatc tccgggctat aaacaggatg gcgcattaag ccgacgtcga gttcaccacg
    38401 gcgaattttt tcctcctgtt gcgtggtgat taaactcacc agctcaatca aggtgtctgg
    38461 ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa ttcacttccg ccgatggcac
    38521 aaagccaatg gttaattgtc tgtcttcctg aacaattttc cgcgcccgta atttggcatt
    38581 ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa cattctcccg ccgccgtcaa
    38641 cgcgactttg cgcttatccc tcaccagtaa cggaacaccg acacagtttt caagatcgcg
    38701 gatctggctg cttaacgaag gctgtgaggt atgcagtttt tccgccgcac gggtaaagtt
    38761 cagtgcctgc gccactgcga cgaaatagcg taaatgccgt agttccatca ccttcccctt
    38821 gttatcgaaa aaacgtctca gctggtagaa attaactatt tcacatatta gcaaccaacc
    38881 agcaacatcc ttatggcaca aaaatagaag gtcaatacat cttatctttc aggattaaaa
    38941 aatatgacca caccctcaga tttgaacatt taccaactga ttgataccca aaatggtcgg
    39001 gtcactccgc gtatttatac cgacccagat atttaccaac tggagcttga acgtattttc
    39061 ggccgttgct ggttatttct cgcccacgaa agccagatcc caaaacctgg tgatttcttt
    39121 aacacctaca tgggggaaga tgcggttgtc gtagtgcgtc agaaagacgg cagcattaag
    39181 gcgtttctca accaatgccg ccaccgggcc atgcgtgtga gttatgcaga ttgcggcaac
    39241 actcgcgcct ttacctgtcc gtatcacggc tggtcttatg gcattaacgg cgagttgatc
    39301 gatgtaccgc tggaacctcg cgcctaccca caagggttgt gtaaatccca ctggggacta
    39361 aacgaagttc cttgtgtgga gagttataaa gggctgattt ttggcaactg ggataccagc
    39421 gcaccgggcc tgcgtgatta cctgggtgac attgcctggt atctggatgg catgctggat
    39481 cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa agtgggtgat caactgtaac
    39541 tggaaattcc cggcagagca gttcgccagt gaccagtatc atgctctgtt cagccatgct
    39601 tctgccgttc aggtattagg ggcgaaagat gatggcagcg ataagcgcct cggtgatgga
    39661 caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc tgcaatttgg tcaggacggt
    39721 cacggtagcg gtttcttctt tactgaaaaa ccggatgcta atgtctgggt cgatggcgca
    39781 gtttcaagct attaccgcga aacctatgcc gaagcagaac aacgtttagg tgaagttcgc
    39841 gccctgcgcc tggcgggtca taacaatata ttccccacgc tttcatggct aaacggcact
    39901 gccacactcc gcgtctggca tccgcgcggc cctgatcaag ttgaagtgtg ggcgttctgt
    39961 attactgaca aagccgcctc cgatgaagtt aaagccgctt ttgaaaacag cgccactcgt
    40021 gcttttggtc ctgctggttt tctcgagcag gatgactcgg agaactggtg tgaaatccag
    40081 aaattgctta aaggccaccg cgcccgcaac agcaaactgt gtctggaaat ggggcttggt
    40141 caggaaaagc gccgcgacga cggcattcct ggcattacta actatatctt ttcagaaact
    40201 gccgctcgtg gaatgtacca acgctgggct gatcttctga gtagcgaaag ctggcaggaa
    40261 gtgctcgata aaaccgccgc ttaccagcag gaggtgatga aatgagtgcg caagtttcac
    40321 tagagttaca tcaccgcatt agccagtttc tctttcacga agccagctta ctggacgact
    40381 ggaaatttcg tgactggctg gcgcagctcg acgaagagat tcgttacacc atgcgcacca
    40441 cagttaacgc gcaaacacgc gaccgccgca aaggcgtcca gccaccgaca acctggattt
    40501 ttaatgacac caaagaccag ctggagcggc gaatcgcccg tctggaaacg ggcatggcct
    40561 gggcagaaga gccgccgtca cgcacccgtc acttaatcag caactgccag ataagcgaaa
    40621 ccgacatccc aaacgtattt gctgtgcggg taaattatct gctttatcgg gcacaaaaag
    40681 agcgcgatga aacattctat gttggaacgc gtttcgacaa agttcgccgt ctggaagatg
    40741 acaactggcg cttgctggaa cgggatatcg tcctggatca agcggtaatc acttcccata
    40801 acctgagtgt actgttctga tgaatcgaat ttatgcgtgt cccgttgcgg atgtgccgga
    40861 gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc ctgttcaacg ttggcggcga
    40921 gttttatgcc attaacgatc gttgcagcca tggtaatgcg tcaatgtcag aagggtatct
    40981 ggaagatgac gccacggtgg agtgcccgct acacgccgcc agtttttgcc tgaaaacggg
    41041 gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact tatccagtac acgttgaagg
    41101 tggtgacatt ttcatcgact taccggaggc gcagccatga gcgatctgca taacgagtcc
    41161 atttttatta ccggcggcgg atcgggatta gggctggcgc tggtcgagcg atttatcgaa
    41221 gaaggcgcgc aggttgccac gctggaactg tcggcggcaa aagtcgccag tctgcgtcag
    41281 cgatttggcg aacatattct ggcggtggaa ggtaacgtga cctgttatgc cgattatcaa
    41341 cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg attgttttat cggcaatgca
    41401 ggcatctggg atcacaatgc ctcactggtt aatactcccg cagagacgct cgaaaccggc
    41461 ttccacgagc tgtttaacgt caatgttctc ggttacctgc tgggcgcaaa agcctgcgct
    41521 ccggcgttaa tcgccagtga aggcagcatg attttcacac tgtcaaatgc cgcctggtat
    41581 cctggcggcg gtggcccgct gtacaccgcc agtaaacatg ccgcaaccgg acttattcgc
    41641 caactggctt atgaactggc accgaaagtg cgggtgaatg gcgtcggccc gtgtggtatg
    41701 gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg aaacctcgat aatgcagtct
    41761 ctgacgccgg agaaaattgc cgccatttta ccgctgcaat ttttcccgca accggcggat
    41821 tttacggggc cgtatgtgat gttggcatcg cggcgcaata atcgcgcatt aagcggtgtg
    41881 atgatcaacg ctgatgcggg tttagcgatt cgcggcattc gccacgtagc ggctgggctg
    41941 gatctttaag gaagcacgat gaaagaaaaa accatcatta ttgtcggtgg cgggcaagcg
    42001 gcggcaatgg ctgcggcctc gctacgccag caagggttca ccggtgagct gcatctgttt
    42061 tccgatgagc gacatcttcc ttatgaacga cctccactct cgaaatccat gttgctggaa
    42121 gattccccgc agttacagca ggtgttaccc gctaactggt ggcaggaaaa caatgttcat
    42181 ctgcattccg gtgtaaccat caaaacgctg ggtcgcgaca cacgagagtt agtgttaacc
    42241 aacggcgaaa gctggcactg ggatcagctt tttatagcaa ccggcgcggc agctcgaccg
    42301 ctgccgttgc ttgatgcact gggagaacgc tgctttaccc tacgccatgc cggtgatgcc
    42361 gccagactgc gagaagttct gcagcccgaa cggtcagtcg tgattgtcgg tgccggaact
    42421 attggtctgg aactggctgc cagcgccacg cagcgcagat gtaaggtgac agtgattgaa
    42481 ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg tgcaacgcta tcttttacag
    42541 cgccaccagc aggctggtgt gcgcattctg ctcaataatg ccattgaaca tgtggtcgat
    42601 ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc ttcaggctga tgtggtgatt
    42661 tacggtattg gtatcagcgc caacgagcaa ctggctcgcg aggccaacct tgatactgcc
    42721 aatggcattg tcattgatga ggcttgccgc acctgcgatc ccgcgatctt tgccggtggc
    42781 gatgtggcaa tcactcgtct tgataatggt gcactacacc gctgcgaaag ctgggaaaac
    42841 gccaataacc aggcgcaaat tgccgctgcc gcaatgttag ggctaccgct accgctactg
    42901 ccgccgccgt ggttctggag cgatcagtac agtgataact tacagtttat tggcgatatg
    42961 cgtggcgatg actggctttg tcgtggcaac ccggaaactc agaaggcgat ttggtttaat
    43021 ctgcaaaacg gcgtgcttat cggtgcggtc acgctgaatc aggggcgtga gattcgccca
    43081 attcgcaaat ggatccagag cggcaaaacg tttgatgcga aactgctgat agatgagaac
    43141 atcgcgctta aatcactgta accaggataa ttagcgaata tctcaatgcc tggggcgtgg
    43201 cgaggtgcaa gagtgtgtat tacgtttaaa tcacattatc ttgcaaaggg attggttatg
    43261 aacacactac gttattttga ttttggagct gcccgccccg ttttgttatt aattgcccgt
    43321 atcgccgtgg tcttaatttt cattattttt ggttttccca aaatgatggg ctttgacggt
    43381 acggtccaat atatggcctc gttgggcgcg ccaatgccga tgctggcagc gattattgcg
    43441 gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg gctttttcac ccgtccgctg
    43501 gcggtgctgt ttattttcta cacgctgggt acggcggtga ttggtcacca ttactgggat
    43561 atgaccggcg atgcggttgg gccaaatatg attaatttct ggaagaatgt cagtatcgct
    43621 ggcgcattcc tgctgctggc aattaccggg ccgggggcaa tttctctcga tcggcgttag
    43681 gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt taataaatta cagccactct
    43741 acccgcaacg acatctctga agttgttgat tcccctggtg ctagcgcaat gagatcaccc
    43801 ccttccgggc gatgatgatc atccggcgca tggctcatcg gttccagaca gaagaaatca
    43861 aacgcatatc ctttatcaaa cgcagggtcg gaaacaaaga tgaaataaca cggtgcaggt
    43921 ggcgtcgttt ccatgatgat ggcataacct tcctgcggct gctcgatgcg ggcctgacca
    43981 ttccatccgg caaaaccatt gttcacccac tggcgcggca acggcgcggg ctggttaaaa
    44041 tccagttcct gcggtagctg ctcgcaaaac tcacccgcca gccactgctc ccgctccagc
    44101 cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg acaacggaaa ataaggatgc
    44161 cagccggtac caaatggcag cgtctctgcc ccttgattgg tgacagagag cgtcaccgtc
    44221 agcgtatccg ccgttaaatg aaacgcctga cttacccgat agtgatagac accgctgcga
    44281 tgttcataca ccagacacaa actatcatcg ctgtgcgaga cacattgcca ctcgcccagc
    44341 cagccatcgc cgtgtagata gtgcgcatcc cactcaacat tcggttgcag ttgatactca
    44401 cgcccctgcc agacaaaacg attgccgctc acccggtttg caaatggcac cagcggaaaa
    44461 catgaggcat cggttgccac accgcttttt ttaccaggac gtaagagcgg cgtcgtatcg
    44521 cgccagaagc cttcgattac gccgccctgg tcagaaacgt ccagctttag cgacccgtgg
    44581 gataaggtat agatggtcat ccgctaactc cttaatccgg gaagttaatc acaactttcc
    44641 cgcatttgcc gctcgccatc agcgcatagg catcacctgc ctgttccagc gagaagcgat
    44701 gggtaatggc gttacgcggc cacagtttcc agtccgtcag atcatgggcg catttttcca
    44761 tatggaacag actggtcacc caggagccga taatccgccg ttgatggtgc atcagatcgg
    44821 cgctgacctc gaactccact tttccggttt ctccgatgta aaccacccgt ccccagtcag
    44881 cggtggatcg cagtgccagc aagcgacctg cggcattacc agaacaatcg agcgcaacat
    44941 ccgcgccacc gtgggtgagt tcggcgataa tctgcggcag accttcggtg gttgctaaat
    45001 agccgtgatc catcacccct aactgttttg ccatcgccag acgttccggc agcatatcaa
    45061 cgccgatgat ccgttttgca ccgcgacctt tcgccagcat catcgccatc atgccgactg
    45121 gccccagacc gaccaccagc acgttatcac tgccggaaac ttcgccgcgc aaaattcctt
    45181 catacgctgt accaacgccg caactgataa acgcaccatc ttcgtagctc agcgcatccg
    45241 gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg ccgtcacgct
    45301 gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg cggcggcagt
    45361 tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc tctttaaaat
    45421 ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg ttgataaatc
    45481 cctggtataa cggtttatcg ggtgccgccg ccgtggcacg gtgttgatga tagatatagt
    45541 ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg ttaatccccg
    45601 gcgtcggcac cgcaacttcc cgcagatcga cggttgaatt tcctggtaaa taagctgcca
    45661 gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct gccgcacccg
    45721 cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca gcaagatatt
    45781 cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa aggagttaat
    45841 accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa gcaatgtacc
    45901 gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg cggcgatgac
    45961 gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgtg ccgccagcaa
    46021 aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc gcactcgacg
    46081 aacgttaata ccgcacaact gcgccgccgt ggcattaccg ccaacggcaa aaaccgagcg
    46141 cccgaaggcg gtttttcggc taatgaacac aaacagcgca aacaacacca tcatgatcag
    46201 cgcggatact ggcacaccga gaaattgtcc gcccagccag tccagcacct cgttttcgtc
    46261 aatcggcact ggcagtgcgt tggtcataaa cagccccatt ccgcgcaggg cgctccacag
    46321 ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc cggcgagcgt
    46381 tcccatcagc gcgcctaaca gcaacaccag cagacacgcc accgccagcg gaacttcaaa
    46441 ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa cgctgacatc
    46501 aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa tggtggcggc
    46561 atcgcgcagc acgttcatct ggttattcaa tgagataaag cctggcgcgt tcagggagaa
    46621 gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat ggcgactgac
    46681 aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag cagacatctt
    46741 caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca gctcatccac
    46801 attgaccgga gcgtgaaact cctgcgagaa cgtgccgtgc tgtagtaaca ggatgcggtc
    46861 acagaccagc ggcaactcct ccacttcact ggagataaac accacgcttt ttccttcggc
    46921 agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga cgccgcgcgt
    46981 tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga tcaccacttt
    47041 ttgctgattg ccgccagaaa gcgtgccgat gggcgtttcg ctactggcgg ccttgaccgt
    47101 catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca gtacgccgtt
    47161 ggcgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca accagggaat
    47221 gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca gcatgtcgcc
    47281 gtaatcgggg cgcgtgattt tctcgccgtt gataacaatt tcgccctgtt catactcctc
    47341 cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc cagcaatgcc
    47401 aagcacttcg ccgcgacgta acgtaaagct gatatcctcc agcttgggct tatggcgtaa
    47461 tgcacggact tccagcacgg cctgatccac aatttcctga ggggcaaccg gcgcaatatc
    47521 aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt tttcgagcat
    47581 cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa tgcggcgaat
    47641 ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca tctttttcac
    47701 cgcgatgatc accagttcaa cttccgcact cgcaagcgaa ctggtaggtt catcaagaat
    47761 gaccacgcgc ggctcgccct tcatcacccg cgcaatttcc accagttgct tttgcgccgg
    47821 gcttagcgtt gaaacaagtt gttcagggct aacgtcaacg cccagcgcct gtaagcaacg
    47881 ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcggg gccactgacc
    47941 gaggcagagg ttttccgcca ccgtcagtcc ctccaccaga cttaactcct gataaaccgc
    48001 acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt ccagtcgcgt
    48061 ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagca ttcgaatgag
    48121 agtcgatttg cccgcgccgt ttttacctaa cagcgcacga acttcgcctt tattgagcgt
    48181 gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg ccaccacttt
    48241 tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc agcgattagg
    48301 gcagaccatc aacgtgcgtt gccagccact gtttaccatc ttccgttttg gtatagagat
    48361 cgattggcac ctgaatcact ttttcaccgt cggcttgttt attgataacc ttcaatgttt
    48421 gcgcgaaaac agcattgccc attttcttac cggaaatatc cactaccgct ttcagcacct
    48481 gattgttttc cagctcctga gcaatttcgg ttgtcatatc cgaaccgaaa acagcaattt
    48541 ttccggcctg attttgatta cgtaccgctt ttaccgcgcc gagtgtcgca ccgcccgact
    48601 cccccataat ggcgttgaga tccggcgtgg agataatcag tttttcacca acggaaatcg
    48661 ctttatctaa aacagtccct tcctgattag cgacaatttg cgcgccggga acgcgggatt
    48721 ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac ttcaaaggct tcgcaattga
    48781 tgacagcaat tttcggctgg tcaattttat tggcaataaa ataatcggcg gcagcgttac
    48841 ccagtttttt accaaattcc agcggatcgc cgaccagata cgccgagaca tatttatcgc
    48901 cccccttttg attaatacag gtgttgtagc aaatcaccgg aatacccgct tcactggcgc
    48961 gacgaacggt acggctactg ccattttcag acactgccga taaaataatg gcatcgacat
    49021 tacgcgccac gagggtatca acaaaggtac tttctttcga aatatcaccc tgggcgttag
    49081 tttcaattaa ctgtacctgt actgaagaat cttttgccgc atcctgaacg ccctgacgca
    49141 ctccagcgta atatccctgg gtatcaaggt atattgcacc aatggtcatt tctttatccg
    49201 cagccctggc aaatagtgcg ctacctaata gcgtagccat taataataga ttacgggttg
    49261 ttctcatttt tttaggcata gtgcctcctg tagggttttt attaacaacg gcttattcta
    49321 attattttgt gatgagcggc agcgcgtgcc gctcaaatat tacagttaat agggtttagt
    49381 gaacgtagtt aaaaataaaa ggcatcacgg tggtgaataa tgccgctttc ggccaggccg
    49441 gattaatcgc cgtcaattca gcgcgagcag attcatattg cgcaacctcc ccgagtcccg
    49501 cggacgccag catcgccacc atcaggcatt tttctttatg ctgctgttgt aaatcgccat
    49561 acagcgacaa caggtcaggt tgtgaaacag caaagaaatc cgcctcaata ctggttttcg
    49621 ccatctcttg cgcccactgt ttcatttcac taaacagttg ctgtgcggtt tgctgttcac
    49681 ccagcagtcg cagcgccatt ccttgccaga agagataatc aaccggctga tcgttgtaat
    49741 aactgtggat gttaatggtg cgatcgccgg tcgccgccag acgtaaacaa cgcatcgctt
    49801 cagtttcatc gccctgcgcg ttggcgcata tcgcctgcca gaaccagatg tcgttatcag
    49861 tttgccccgg taaacggcct tcgcttaaat tctccggata atgcagcgcg gcatgaagca
    49921 gttcgctggc ctgctgcggc tctctggcat caagatgctg ccaggcgcgt aataactggt
    49981 tgaggataaa ctgactggtg accttccctt ccccgccttc ccacgggtgg aatttacgcg
    50041 tggcgagaat gtccgccgct ttgtctgcct gacccgtgag atgccacaaa ttgagcagtt
    50101 ctgcggtcat gtcgtcgcgt ttcagcgcaa tttcctgatt attttccaga cgcgccagtc
    50161 gtttctccgg tgtggctcca cttaacttat caagcaaatc ccgttcgaaa agcagacgtg
    50221 catcctgcgg cgcaagctga taagcattat caagataacg cgcggccagc tcataatcgt
    50281 gttgcttatt ccacgcatgg atcgctaacc cgcgccagcc gtcggcaaac tccggcgaca
    50341 tctctacgca acgttgccat aaggcaatgg ctttgttgta gctacgtttg ttgtagtaga
    50401 agcaggccag taaatggcga gcaaaccagc actcttcaat actctccagc gccgccactt
    50461 cttccagcgt attcgggaaa cggacaaact gcgggaagac atcaatggct tttgcgacca
    50521 gttcgccacg ttcggctttc ggcagcaagc tggcttgcag gtaaagcggc agcgtgcgct
    50581 ggcagtccag tgcgttcagc atctctgccg ccagggtggg cattccccag ttaatcagtt
    50641 gcccggcggt catcagggcg ttaacgtcgc gtccctgaca cagaccgcgc cactgcgcga
    50701 gagctgattc gctacgacca tcgaaccagt tcagccacca cagagtggcg ttcagcggat
    50761 aatcgcgcag cagtttctcg cgctgcacac gcgcgttgtc ctgacgacca cttaacacca
    50821 gcagcagatt atgcaggcaa agcacttcct gattggttgg gcaggcgcga agactttgtt
    50881 ggcaaaaatc cagaccagcg tcgaagttac cattacgcgc cgccagtcgt gccaggccat
    50941 aatagccacc ggctttgctg ttgccgctcc agaccgcacg ccagaaatcc tcttcggctt
    51001 gttgatattg tccctgacgt tcgtaagcac tggcgcgaat caaactcgcc tgtccgcact
    51061 gcggattttt gttcagcgca tgtgcgcgtt tcagagcctg agtggcatac gccaccgctt
    51121 gcgggaaatc ggcgcggtta tattccagca tcgccagcgc caggttacag cggtaatcca
    51181 gcggatccag cgccacgccg cgcaggtagt aatcgaacgg tgaacggctg gcgtgatgat
    51241 attgctccag atgctgaccg ataaaccagg cttcatctgt actggtaatg tcttgtgctg
    51301 ccagtggcgc tttggcgacg tccggcaacg gcaactcttg cggctgatgt tcctgataac
    51361 tcagtacaat attgccgtcg gcatcggaga gctcaatggt cagcctttca ggattgatac
    51421 cgtgcaacac gccctggatg gcggtcgcag gcatcagtgc cacggcatca tcaagtaacg
    51481 cgttgcattt gccgatttcg cggatcgcca ggcggtatcc gttcaacgga gagatggcat
    51541 acagccccca ctcaatcccc cgcttactac gctggagttt tatcaccgca tcgcgggagg
    51601 cattttgcac catgcccaaa gaatgataag gcaggaaata ctgctcaaaa cgcttctctt
    51661 cgtaagcatc aagccaggta aaatcgggct ggttatcggc aaaaataccg gtcatcagtt
    51721 cgatatacgg accgttattg tcggtcagac tcttatccca cgcctggcca aattcactgt
    51781 gtccccaact ccactgtttt ttacctggcg caatatggtg gttggcaacg tgcagcaaac
    51841 cgccatcttc atcgtgacac cacgcgccga caaaatcgta ctgcgatttt tcggccatat
    51901 atgaggttgg aacaggcaca tttttatagc gagaaatgtc cactccggcg gagtagtcca
    51961 ctttgtagta agtgccggtg gcgatgggga aagcggagac ggcccgtttg ccgtgatcaa
    52021 acaccgcagt cacatccggt gggaaaacgc tctgatgccc ttccccccct ttcactgccg
    52081 ggttggccca ccacaagaaa tgacgcggcg tggcgttacc gttatagacg cggctggcga
    52141 tttccagcgc cgcccggtca gggcgcaggg tgaaacctgt catcacctgt aaaccatgca
    52201 tcggctccgt ttcgcctacc cacaccgtct gtgcaccgtc ttcatgggct tcgagggtga
    52261 aatcaacggg cataaaggtg gtcgggcgat gatgttgcgg ccagttaaac tcaatcccac
    52321 cggaaatcca cggccccagc agccccacca gcgcaggttt aatgacttca ttgtgataaa
    52381 caaaatcgcg ctgtttcact ttatcccacg cgcgatgcac ccgaccgccc agttccggca
    52441 ggatcatcac tttgatgtag tcgttttcca gccacaccgc ctgccaggat ttcagggttt
    52501 tctgctcgct cagcgtatcg gtcacgccgt agggataaac cgcgccggac gatccctgat
    52561 aaacgcgatt ttcgaggaac atgggatgta tatcctgcgg cccggtttca taggtcggga
    52621 tctcaacgcg ctcttgccac acttttactg gagtcatggt gccctcaata ttaacaagac
    52681 atactgaatt aaaagatttg tggcagtgta ttgaacaatc tggcaatgtt ttcgcggaat
    52741 aatcacgcaa ttaactaaac aaggtttagt gaagatgaga gcctgcatta acaatcaaca
    52801 gattcgccac cataacaaat gcgtgattct ggaactgctg taccggcaaa agcgcgccaa
    52861 taaatcaacg ctggcccggc tggcgcaaat ttcgattccg gcagtcagta atattttgca
    52921 ggaactggaa agcgaaaaac gggtggtgaa tattgacgat gaaagccaga cgcgcgggca
    52981 tagtagcggt acatggctga ttgcgccgga aggtgactgg acgctgtgcc tgaacgtgac
    53041 gcccaccagt attgagtgtc aggttgctaa tgcttgttta agtccgaaag gtgaatttga
    53101 gtatttacag attgatgcac cgacgccgca ggcgctgctg tccgaaatcg aaaaatgctg
    53161 gcatcgccac cgtaaattgt ggccggaccg caccatcaat ctggcgctgg caatccacgg
    53221 tcaggttgat cctgtgaccg gcgtgtcgca aaccatgccg caagcgccgt ggacaacgcc
    53281 ggtggaggtg aaatatctgc tggaagagaa gctcggcatt cgggtgatgg tcgataatga
    53341 ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat tcgcaggaac gggatttctg
    53401 cgtgatcaac gttgattacg gcattggctc gtcgttcgtg attaacgagc aaatttatcg
    53461 cggcagtttg tatggtagcg gacagattgg tcacaccatc gttaatccgg atggcgtcgt
    53521 ctgcgactgt ggacgttatg gctgcctgga aaccgtcgcc tcgttaagcg cattaaaaaa
    53581 acaggcgcgg gtatggctaa aatcacaacc ggttagtact caacttgatc ctgaaaaact
    53641 gactacagcg cagttaatcg ctgcctggca aagtggagaa ccgtggatca ccagctgggt
    53701 tgaccgcagt gccaatgcca ttggtttgag tctgtataac ttcctcaaca tcctcaatat
    53761 taatcagatt tggttgtacg gtcgcagttg tgcctttggt gagaactggc ttaatactat
    53821 tattcgctag acaggattta acccgttcga ccgcgacgaa ggaccgagcg tgaaagcgac
    53881 gcaaattggc ttcgggcaat taagccgcgc acaacaggtg ctgggaattg gctatttgta
    53941 tgttgaggcg cagttacggc agatttgatg gcgcaataac gtagaaaggc ttcccgaagg
    54001 aagccttttt tcaacagatt aaccgtttcg cttatgcgta aaccggataa cgtgcgcaga
    54061 tgtcgagaac tttacctttg atgcgctcga taacagcttc atcattgatg ctgtccagca
    54121 cgtcacacat ccagccagcc agttctttcg cttctgcttc tttgaagccg cgacgcgtaa
    54181 ttgccggagt acccacgcga ataccggagg tcacaaacgg gctcttcgga tcgttcggta
    54241 cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct tctttaccgg
    54301 tcaggttttt atcaaccaga tcaaccagga acaggtggtt atcagtgccg ccggaaacca
    54361 ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt agcgtttttc gcgacctgct
    54421 gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct ttaccggcga
    54481 ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc agttttttgt
    54541 acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc gccagggttt
    54601 tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag acgccagcag
    54661 caaccaggcc cgcaacgtgc gccatatcaa cgaacaggta agcaccgatg ctgtcagcga
    54721 tttcacgcat tttcgcccag tccaccacgc cggaatatgc agagaaacca ccgataatca
    54781 ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtagtcgata tgaccggtag
    54841 catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc ggagaaccgt
    54901 gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct ggttccagca
    54961 gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg ttagcgtagt
    55021 cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata tcaacatact
    55081 cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg gtcagctgag
    55141 aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc agttcgatgt
    55201 gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca tcataatcgg
    55261 caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa taaaattttt
    55321 ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc attgtgcgat
    55381 aacaggtctt gacaaaggaa tttacgcaaa cgattacctt caggctacgc aaggctttgg
    55441 agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg caaatttttc
    55501 acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag ggctattttt
    55561 tataagatgc atttgagata catcaattaa gatgcaaaaa aaggaagacc atatgcttga
    55621 cgctcaaacc atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa
    55681 gttaaccgcc catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt
    55741 taacatgagt aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc
    55801 ctacgccagt aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca
    55861 gaagcacacc agcttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt
    55921 ggcaacgctg gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc
    55981 ctatggtgta ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc
    56041 cagcaaagcc ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg
    56101 cagcgcgctt atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata
    56161 ccgtccgggg caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat
    56221 tcgtcagtac tctttgactc gcaaaccgga tggcaaaggc tatcgtattg cggtgaaacg
    56281 cgaagagggt gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt
    56341 gaaactggtc gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac
    56401 gttaatctct gccggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa
    56461 agcaggccac acagcacaag tgaactggtt ccatgcggca gaaaatggcg atgttcacgc
    56521 ctttgctgat gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg
    56581 gtatcgtcag ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat
    56641 ggatttgagc aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg
    56701 cccggttggc ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga
    56761 aaacattcat tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg
    56821 gaaacatccg gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg
    56881 gatgcgaatg acccgtgcca cgtcgaagac gaagatttta ccgtcaccga ttttgccggt
    56941 ttgcgccgtg cgaataatgg tatcaacaca ggtatcgaca atgtcgtccg gtacgacaat
    57001 ctcaattttc actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt
    57061 atggcctttc tggcggccaa aacctttcac ctcagtgacc gtcatgccgg taatgccgac
    57121 ttcagccagt gcttcgcgaa catcgtccag cttgaagggt ttaataatcg catcaatctt
    57181 tttcatgcta ttccttgaaa aggtcgcctg tcttttgatc tgctaaacgt aacacataac
    57241 gccaattcat tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa
    57301 ttctgtccgg ttgcgccccg ccattctcgc cgcgtgtgtg acgttgcctt tggtgatttg
    57361 cagcagctta cgcaaatagt tgagttcaaa ctgattacgt gcctcaacaa aggttggcag
    57421 cgccgtattt tcaccctcca gcgcctgctc tactaatgca tcgctaataa ccggagatga
    57481 ggtcagcgcc acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct
    57541 agcggtcatc aggcgtttca tcgcatcagt ggagaacgcg cggacaaacg gtttatgtcg
    57601 ctctgccgcc tggcgcaaca ggtgatttgc cagaagcgga atgtcttctg tgcgctctgc
    57661 cagagccgga attttcaggc tgacaacgtt gaggcggtaa tagaggtctt cacggaattc
    57721 cccgcgcgcc atcgcttttg gcaggtcgcg gtgagtggcg gaaataatcc gcacgtcgat
    57781 atcaatatcg cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag
    57841 tttgacctgc aacggtgcgg gcatatcgcc aatctcatcg agaaatagcg taccgccttc
    57901 cgccgcctgg aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg
    57961 accaaacagc tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa
    58021 tggtttgcta ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt
    58081 gccgctctgg ccgttaatca aaacgctaac gtctgattgc gccaccagcc gcgcctgttc
    58141 cagcaaacgc agcatcagcg ggctgcgggt gacaatcgcc tcgcgccagc gttcatcggt
    58201 ggctggcgcg gattgttcca gcgcatcgtc aattgcctga tatagcgcat ctttatcgac
    58261 aggcttggtg aggaaactga acacgccctg ctgtgttgca gcaacggcat cgggaataga
    58321 accatgcgcg gtaagaataa ttactggcat tcccggctgc actttctgaa tttcagcaaa
    58381 cagctgcata ccgtccattt catccatccg caggtcgctg atgactaaat ctactttttc
    58441 gcgattcagt acccgtaatc cttcagcgcc actttccgcc gtgaccacgc tgtagccttc
    58501 gctggtcagg cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa
    58561 atgcgcaggt ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac
    58621 cctcgtgcgg cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt
    58681 caatatcagt caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt
    58741 gatgttgctg gcgcaatgta tccagctcgc tgtcgctggc ctgctggagc ttgccgtagc
    58801 gttggcgctc ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca
    58861 gagggcgaac ctgtgccggg atctcggtac ttagcgcatc aatgcgcgcg accagctggc
    58921 ggcgctcata cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt
    58981 gccagctgcc gtcgtcatat tggcgagctt gctgacgtga ctgcgcaggc attaaacgat
    59041 cagcacaatc catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca
    59101 gcgcccagat gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt
    59161 tttcttctgc tggcgtatca atggctggct tattgtgact ctgcacgcaa cccagcaatg
    59221 ccagacatgg cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc
    59281 gcatattcac cagacttaat gcctatccca gtgggcgtaa ttattgcagg cagtttggac
    59341 atggacagcg cggagaaacc ggagcgtaca cgtcgtacgt gaggatttcg agcactgccc
    59401 ggggccgaac tggcaaataa aatagcctga tgggataggc tgttagcatt atttcgtgtt
    59461 tttcgacgac ggcaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata
    59521 cagttcccct tgcatacggc aaatacaatc cctggcaatg cttaatccca gaccgctgcc
    59581 cttcaccgcc ccttttcgct ggtggcttcc ttgaaaaaag ggttcgaaga tcatggcgcg
    59641 ttcctcttgc ggaatgggtg tgcctgtatt gatgacatca atataaaccc gtgcaccatg
    59701 taagctgctg cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta
    59761 aagattatcc agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag
    59821 atcgacgtcg gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt
    59881 ctccaccagc ggtgctaatt caacattctc cagttccacc gcactgtccg cctgtttacg
    59941 gttgtaatca agcagttgtt cgatcagttt ttgcaaattg cggctgctgc tatcaagaat
    60001 gctcaccacc tctttttgct ctggcgtaag cggcccgaca acctggtcag ccagtaattc
    60061 agtgccctcg cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa
    60121 ttgatggcgt tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc
    60181 aaccgagcgt aactcgctcg gtccactgaa cgaaacgcta ttgcccagag aacgcccttc
    60241 ccccagacgg ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa
    60301 aagtagcacc atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg
    60361 cccacgttcg gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt
    60421 ggcctgcacc atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc
    60481 atcgggacca ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc
    60541 ctggtaaagt ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg
    60601 cttgcgctgg ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc aatactgacg
    60661 gtagctacgc tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat
    60721 aagcgtagtg cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc
    60781 ttgccatgcc agcaccaaca ggggtagtag aattagcaaa aatgccagca ttaccagttg
    60841 tcgtaatgag cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa
    60901 tagcctgatg ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt
    60961 gttatggaat aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct
    61021 gatatcagaa gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcaca
    61081 tcccgaaggg gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg
    61141 tgccaataaa gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg
    61201 gcttcttcgc tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga
    61261 taaacttatt agattacaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta
    61321 aaccacccat tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat
    61381 agtggttggt gcttaatgcc ggatgcggcg taaatgccgc atccgggctg caaaaccaat
    61441 gaactgatga cttaccccaa ctgcttacgc gcattgcgga aaatgcgcat ccatgggcca
    61501 tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac acgttccggg
    61561 tgcggcatca taatggtgac tcgaccactt tcagtcgtaa ccgacgtaat accgttcgga
    61621 gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc gacatagcgc
    61681 agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg cacttccacg
    61741 cgcccttcac cgtgagagac agcaatcggc atttgcgagc ccaccatccc ctgcaacagc
    61801 agcgacgggc tttgggtcac ttcaaccagg ctgaaacgcg cttcaaagcg atcggaggta
    61861 ttgcgcacaa aacgtggcca caactcacta cctgggatca gttcacgcag attagacatc
    61921 atctggcaac cgttacatac ccccagcgcc agcgtttgcg gacggtggaa gaaggttgca
    61981 aactcatcgc gtacacggtc attgaacagg attgacttcg cccaaccttc accggcaccc
    62041 agcacatcac cgtaggagaa accaccgcac gcgaccaggg cgtggaaatc ttccaggccc
    62101 gtgcgtccag ccagcaggtc actcatatgc acgtcgatag catcaaagcc tgcacggtgg
    62161 aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagtac ggcaactttc
    62221 gggcgcgcgc cagtggcaat aaacggtgct gccacatctt cgttgatatc gaacgacagt
    62281 tttacattca ggcccggatc ggcgtcgttt gatttcgcct ggtgctcctg gtcagcacac
    62341 tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca gacacgcaac
    62401 gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa acggtcaccg
    62461 gaaaccgcct gcccaacata atggacgcaa tcagcaagcc catgctgtgc cagtacggac
    62521 tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg cacccagttc ttcgttaaac
    62581 aacgccgcca ggcgatcgtc acccagagag ccgatatccg catcaatgcc acaatgacca
    62641 gcaaaggcca tttccgccag tgtgaccaac agaccgccat cagagcggtc gtgatacgcc
    62701 agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt cagttgcgcg
    62761 acatcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc cagcgccgtt
    62821 gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt atcttcggta
    62881 gaaagctgcg gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc agaaatcacc
    62941 agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg ggttttcatc
    63001 gacatagagt ctttacccac cgggatcgtc aggcccagcg ccggacaaag ctcttcgccc
    63061 acggctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc cgccgccatc
    63121 cagttggcgg aaagtttaat gcgtttgata tcgccaatct gtgttgcggc aatattggtt
    63181 aacgcttcac cgaccgccag acgggcagag gcggcgaaat ccagcagcgc aaccggcgca
    63241 cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt agtgaccgcg
    63301 cagttagcga ccggcacctg ccacggaccc accatctgat cgcgcgctac catgccagtt
    63361 acgctgcggt caccgatggt caccaggaaa gttttctccg ccacggtcgg cagatgcagt
    63421 acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga ccagcgcatc gcccttcgct
    63481 ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac caagcaggac gtccagcggc
    63541 agatcgatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag ttcttcggtc
    63601 gcttcaccaa tcaccgcgta gggtgcgcgc tcacgcttac acagttcgtc aaacagcggt
    63661 aattgatcgg cagcaaccgc cagcacgtag cgttcctggg attcgttaca ccagatttcc
    63721 agcgggctca tgcccggttc gtcgcttaga atatcgcgca gttcaaattt accgccgcgt
    63781 ccgccgtcgc tcaccagctc cggcatcgcg ttagaaagac cgcccgcacc gacgtcgtgg
    63841 ataaacagaa ttgggttagc atcaccaagc tgccagcaac ggtcgatcac ttcctggcaa
    63901 cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc ggcatcagac
    63961 tgacctgacg ccatggaaga cgccgctccg ccgccaagac cgatgttcat tgccgggccg
    64021 ccgagaacga ccagcttcgc accgacgttg atctcgcctt tttgtacgtg atcggcgcga
    64081 atgttgccga tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag ctcttcgccg
    64141 ttgtggctgt tcactttttc ttcataagta cggaagtagc cattcagtgc cggacgacca
    64201 aattcgttgt taaacgccgc gccgcccagc gggccttcgg tcatgatgtc cagcgcggtg
    64261 acaatgcgct caggcttacc gaaatcttct tcccacggct gttcgaagcc aggaattcgc
    64321 aggttggaaa cggagaaacc aaccaaaccc gctttcggct tcgcgccgag gccggtggca
    64381 ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc ccggccacgg agaaatcgcc
    64441 gtcgggtggt tgtgagtttc gactttcatc agaatatgcg ccggttcctg atggaaatcg
    64501 tagcggcccg tttcgtggtc agcaaagtag cggcccactt cagaaccttc cattacggcg
    64561 gcgttatctt tataagcaga gagaacgtaa tctggcgtgg tttcgaaggt gtttttgatc
    64621 atcttaaaca gcgatttcgg ctgttgctca ccgtcgataa cccaatcggc gttaaaaatc
    64681 ttgtggcggc agtgctcgga gttcgcctgg gcgaacatat acagctcgat gtcgttcggg
    64741 ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa tttcatcttc cgccagtgcc
    64801 aggccaagac gcaggttagc gtcgattagc gcctgacggc cctgccccag caaatcaacg
    64861 ctggtgaccg gagtcggttg atggtgtgca aacagctgct ctgcatcatc taaagcaaaa
    64921 aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa cctgctgcca ttgttcattg
    64981 gtcagcgtac cggcttctat atagtaagca acgccgcgct caaggcggtt tacctgttgt
    65041 agcccgcagt tatgggcaat atcggtcgct ttcgaagacc agggagagat ggtgccagga
    65101 cgcggggtca ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc cgggccatat
    65161 ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta acggcgcatt gaggtcagca
    65221 aaatggacat actcggcgta aatattgtga accgggagcc tggcagcctg aaaacgtgcc
    65281 agcagtttgt tgattcggaa tgccgacagt gcaggcgaac cacgcagaat ttccatcata
    65341 agtctctcgt cttctaagct ttcggtgtac ccaagggggg gaaacgggcg tcattataaa
    65401 gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg tgaattagca
    65461 acgcgtgccg ccaatggctg taataagttg ccatctggcg caggtttacg caaaatgccg
    65521 ctcatttaag agtaaacctt tcactattat tacgtttttt caagctggga cgcgcacgac
    65581 acagagaatt aactaattga aaaaattaaa gattaattat ctgttcatcg gcattctggc
    65641 actgctgctc gcggtcgctc tctggccatc cattccctgg tttggtaaag ccgacaaccg
    65701 tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc accattcata ctcccctgac
    65761 ttataacgaa atcaacggga aaccttttgg cctggattac gaactggcga aacagtttgc
    65821 cgattacctc ggcgtaaaac tgaaagtgac cgtgcggcag aatatcagcc agctgtttga
    65881 cgaccttgat aatggtaacg ccgacctgct ggcggcagga ctggtctata acagtgagcg
    65941 ggtaaaaaat tatcagcctg gccctaccta ttattccgtg tcacaacaac tggtttataa
    66001 agtgggtcag tatcgcccac gtacgctggg caacctgacg gcggagcaac tcaccgttgc
    66061 accgggtcat gtggtggtta acgatctcca gaccctgaaa gaaacaaaat tcccggaatt
    66121 aagctggaag gtagacgaca aaaaaggctc tgcggaatta atggaagatg tcatcgaagg
    66181 aaaactcgat tacaccattg ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc
    66241 ggagctcgcc gtagcgctcg atatcaccga tgaacaaccg gtgacttggt ttagcccgtt
    66301 agatggcgat aatacccttt ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga
    66361 cggtacgctg gcacgcattg aagagaaata cctggggcat ggcgatgatt ttgattacgt
    66421 cgatacgcgc acatttttac gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt
    66481 tgagaaatac gccgaagaaa ttgactggcg tttgctggcc gctattgctt atcaggaatc
    66541 gcactgggat gcacaggcca cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa
    66601 aaataccgcg caaagcctcg gcattacgga tcgtaccgat gccgaacaga gcatcagcgg
    66661 tggcgtgcgt tatttgcagg atatgatgag taaagtgccg gaaagtgtgc cggagaacga
    66721 gcggatctgg tttgccctcg ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg
    66781 cgccctgacg gcaaaaacca aagggaatcc tgacagttgg gctgacgtaa aacagcgtct
    66841 gcctttactt agccagaaac cctattacag caagctgact tacggctacg ctcgtggaca
    66901 tgaagcctac gcttatgtcg aaaatattcg taagtatcag attagcctgg tgggttatct
    66961 gcaagagaaa gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg attatccggc
    67021 ggtatcgcct acggagttgg gcaaagagaa atttcctttt ctctcgtttc tttcccagtc
    67081 gtcatcaaac tatttgaccc attctccctc tctgctgttt tccaggaaag ggagtgaaga
    67141 gaaacaaaat taatccgtcg aggattgcgc ttttttctgc gctttaattt cctggcggcg
    67201 catgcgaaag aagtcactga gcaacgccgc gcactcatcc gccagtattc cttccgtaat
    67261 ttccactcgg tgattcatac ccggatgatg cagcacatcc attaaagatc ccgcagcgcc
    67321 agttttcgcg tcacgcgcac caaagaccac gcgaccaatg cgactgtgga tcatcgctcc
    67381 ggcacacatt acacatggtt caagcgtgac atacaacgtg gcgtcgatca gacgataatt
    67441 ttgcatcacc agaccaccct gccgcagggc catgatttct gcatgtgcgg tgggatcatg
    67501 gcgaccaatc gggcggttcc agccttcgcc gattacccga ttgttatgca ctaataccgc
    67561 gccgaccggc acttcccgct catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat
    67621 ccagtattcg tggctaaatt cgacttcaga caaaaagaaa actccggtta taaaagcgcg
    67681 gcgcattata cacggacgct atgctttact ctattccagt tgctggagtt caccgcgcgg
    67741 ggttactcgc caacgatgct gacagaaata aagcagcgga ttgtcctgat tactgtcgct
    67801 atagccactg tacagccgca gcggagtgcc gattttgcgc tccagttgtg cgaccttttc
    67861 atgtcccaga caacgcatcg tcaataccca accaccatag ccacgctgaa tttggctggc
    67921 gataagatta acccgcggca gccagggcgt atcgaaataa accgcttcaa ccagcggctg
    67981 cggagagccg gtaatcaacc agatatcagc atcggaactt aacaggtagg tggttaatcg
    68041 ctcctgaacc agcggaaagg cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg
    68101 caacgtctgt aaacgtgctt cgctgtgacc aaaagtgcac ccccacagaa gcagactcat
    68161 cggccagcgt gccgcacgac cttttatcaa taacgcaatg gctataatcg gtaacaacgg
    68221 caggacaagt aacgcattca gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa
    68281 catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac accacacgac gctcgtgagt
    68341 tgccaaacca tactcctctg gactgaaaca tcctgattaa ttttcgctac tacatagcct
    68401 aacagataga tcatcacttt tccggcaaca atcgaccgca tcatggctaa ctggaatttt
    68461 taattcatgg caattagcgg caatggaata taaaattcac tcgcgtgtct ctcatattgc
    68521 ctgttgtcgc cactatttca tcgcgaggaa agatatgaac ggcttacttc gtatccgtca
    68581 gcgttaccag gggcttgccc agagtgataa aaaactggcg gattatctgc tgctacaacc
    68641 tgatacggcg cgccatttaa gctctcagca actggccaac gaagccggag tcagtcagtc
    68701 cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt tttccggcgc ttaagttggc
    68761 gttgagtgaa gcgctggcaa gccagccgga atcgccctcc gtgcccattc ataaccaaat
    68821 ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg attaaagaaa atactgccgc
    68881 tatgtacgca acgctaaacg ttaatagtga agagaaactg catgaatgcg taacaatgtt
    68941 gcgctctgcg cggcggataa ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa
    69001 ctttgcctgg aagctgatga agattggctt caatgctgcc gcagtgcgcg atatgcatgc
    69061 gctactcgca acagtacagg cgtcgtcccc tgacgatctg ttattagcca tttcctacac
    69121 cggtgtacga cgcgagttaa acctggcggc agatgagatg ctgcgagtgg gcggaaaagt
    69181 gctggcgatt accggcttta ctccgaatgc cctgcaacag cgtgcttctc attgcctgta
    69241 taccattgcc gaagaacaag cgacaaacag cgcttcaatc tctgcttgtc acgctcaggg
    69301 aatgttaacg gatttgctgt tcattgcgct gattcagcag gatctggaac tggcaccaga
    69361 acgtattcgt catagtgaag cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat
    69421 aatgcccgcc cggtttgtgt tgttttgaga gtttccttat ggcgttgtta atcactaaaa
    69481 aatgcatcaa ttgtgatatg tgtgaacccg aatgcccgaa tgaggcgatt tcaatgggag
    69541 atcatatcta cgagattaac agcgataagt gtaccgaatg cgtagggcac tacgagacac
    69601 caacctgcca gaaggtgtgc ccgatcccca atactattgt gaaagatccg gcgcatgtcg
    69661 agacagaaga acagttgtgg gataaatttg tgctgatgca ccacgcgga
//
LOCUS       DAZFRJ010000004        64509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000004 DAZFRJ010000000
VERSION     DAZFRJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.004"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..887
                     /gene="focA"
                     /locus_tag="ACHKUN_000210"
     CDS             30..887
                     /gene="focA"
                     /locus_tag="ACHKUN_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO6658539.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            942..3224
                     /gene="pflB"
                     /locus_tag="ACHKUN_000211"
     CDS             942..3224
                     /gene="pflB"
                     /locus_tag="ACHKUN_000211"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6658540.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3416..4156
                     /gene="pflA"
                     /locus_tag="ACHKUN_000212"
     CDS             3416..4156
                     /gene="pflA"
                     /locus_tag="ACHKUN_000212"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO6658541.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4238..4828)
                     /gene="ycaK"
                     /locus_tag="ACHKUN_000213"
     CDS             complement(4238..4828)
                     /gene="ycaK"
                     /locus_tag="ACHKUN_000213"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO6658542.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            4928..5836
                     /gene="ycaN"
                     /locus_tag="ACHKUN_000214"
     CDS             4928..5836
                     /gene="ycaN"
                     /locus_tag="ACHKUN_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6658543.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5837..7158)
                     /gene="ycaM"
                     /locus_tag="ACHKUN_000215"
                     /pseudo
     CDS             complement(5837..7158)
                     /gene="ycaM"
                     /locus_tag="ACHKUN_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(7367..8515)
                     /gene="ycaD"
                     /locus_tag="ACHKUN_000216"
     CDS             complement(7367..8515)
                     /gene="ycaD"
                     /locus_tag="ACHKUN_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6658544.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            8830..9456
                     /gene="ycaC"
                     /locus_tag="ACHKUN_000217"
     CDS             8830..9456
                     /gene="ycaC"
                     /locus_tag="ACHKUN_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO6658545.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9492..10355)
                     /gene="dmsC"
                     /locus_tag="ACHKUN_000218"
     CDS             complement(9492..10355)
                     /gene="dmsC"
                     /locus_tag="ACHKUN_000218"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO6658546.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10357..10974)
                     /gene="dmsB"
                     /locus_tag="ACHKUN_000219"
     CDS             complement(10357..10974)
                     /gene="dmsB"
                     /locus_tag="ACHKUN_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO6658547.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(10985..13429)
                     /gene="dmsA"
                     /locus_tag="ACHKUN_000220"
     CDS             complement(10985..13429)
                     /gene="dmsA"
                     /locus_tag="ACHKUN_000220"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO6658548.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13668..14960)
                     /gene="serS"
                     /locus_tag="ACHKUN_000221"
     CDS             complement(13668..14960)
                     /gene="serS"
                     /locus_tag="ACHKUN_000221"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO6658549.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15051..16394)
                     /gene="rarA"
                     /locus_tag="ACHKUN_000222"
                     /gene_synonym="mgsA"
     CDS             complement(15051..16394)
                     /gene="rarA"
                     /locus_tag="ACHKUN_000222"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO6658550.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(16405..17016)
                     /gene="lolA"
                     /locus_tag="ACHKUN_000223"
     CDS             complement(16405..17016)
                     /gene="lolA"
                     /locus_tag="ACHKUN_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO6658551.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(17171..21277)
                     /gene="ftsK"
                     /locus_tag="ACHKUN_000224"
     CDS             complement(17171..21277)
                     /gene="ftsK"
                     /locus_tag="ACHKUN_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO6658552.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(21412..21906)
                     /gene="lrp"
                     /locus_tag="ACHKUN_000225"
     CDS             complement(21412..21906)
                     /gene="lrp"
                     /locus_tag="ACHKUN_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO6658553.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22450..23415
                     /gene="trxB"
                     /locus_tag="ACHKUN_000226"
     CDS             22450..23415
                     /gene="trxB"
                     /locus_tag="ACHKUN_000226"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO6658554.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            23538..25304
                     /gene="cydD"
                     /locus_tag="ACHKUN_000227"
     CDS             23538..25304
                     /gene="cydD"
                     /locus_tag="ACHKUN_000227"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO6658555.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            25305..27026
                     /gene="cydC"
                     /locus_tag="ACHKUN_000228"
     CDS             25305..27026
                     /gene="cydC"
                     /locus_tag="ACHKUN_000228"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO6658556.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            27068..27772
                     /gene="aat"
                     /locus_tag="ACHKUN_000229"
     CDS             27068..27772
                     /gene="aat"
                     /locus_tag="ACHKUN_000229"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO6658557.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            28057..28275
                     /gene="infA"
                     /locus_tag="ACHKUN_000230"
     CDS             28057..28275
                     /gene="infA"
                     /locus_tag="ACHKUN_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO6658558.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            28529..28616
                     /locus_tag="ACHKUN_000231"
     tRNA            28529..28616
                     /locus_tag="ACHKUN_000231"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28563..28565,aa:Ser,seq:gga)
     gene            complement(28959..31235)
                     /gene="clpA"
                     /locus_tag="ACHKUN_000232"
     CDS             complement(28959..31235)
                     /gene="clpA"
                     /locus_tag="ACHKUN_000232"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO6658559.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(31266..31586)
                     /gene="clpS"
                     /locus_tag="ACHKUN_000233"
     CDS             complement(31266..31586)
                     /gene="clpS"
                     /locus_tag="ACHKUN_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO6658560.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(31685..31759)
                     /gene="yljB"
                     /locus_tag="ACHKUN_000234"
     CDS             complement(31685..31759)
                     /gene="yljB"
                     /locus_tag="ACHKUN_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO6658561.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            31909..32133
                     /gene="cspD"
                     /locus_tag="ACHKUN_000235"
     CDS             31909..32133
                     /gene="cspD"
                     /locus_tag="ACHKUN_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO6658562.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(32206..34152)
                     /gene="macB"
                     /locus_tag="ACHKUN_000236"
     CDS             complement(32206..34152)
                     /gene="macB"
                     /locus_tag="ACHKUN_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO6658563.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(34149..35264)
                     /gene="macA"
                     /locus_tag="ACHKUN_000237"
     CDS             complement(34149..35264)
                     /gene="macA"
                     /locus_tag="ACHKUN_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO6658564.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            35415..36371
                     /gene="ybjX"
                     /locus_tag="ACHKUN_000238"
     CDS             35415..36371
                     /gene="ybjX"
                     /locus_tag="ACHKUN_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO6658565.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(36368..38026)
                     /gene="ybjD"
                     /locus_tag="ACHKUN_000239"
     CDS             complement(36368..38026)
                     /gene="ybjD"
                     /locus_tag="ACHKUN_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO6658566.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            38452..39147
                     /gene="aqpZ"
                     /locus_tag="ACHKUN_000240"
     CDS             38452..39147
                     /gene="aqpZ"
                     /locus_tag="ACHKUN_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO6658567.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            39642..40541
                     /gene="lysO"
                     /locus_tag="ACHKUN_000241"
     CDS             39642..40541
                     /gene="lysO"
                     /locus_tag="ACHKUN_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO6658568.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            40685..42337
                     /gene="hcp"
                     /locus_tag="ACHKUN_000242"
                     /gene_synonym="priS"
     CDS             40685..42337
                     /gene="hcp"
                     /locus_tag="ACHKUN_000242"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO6658569.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            42349..43317
                     /gene="hcr"
                     /locus_tag="ACHKUN_000243"
     CDS             42349..43317
                     /gene="hcr"
                     /locus_tag="ACHKUN_000243"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO6658570.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            43450..45168
                     /gene="poxB"
                     /locus_tag="ACHKUN_000244"
     CDS             43450..45168
                     /gene="poxB"
                     /locus_tag="ACHKUN_000244"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO6658571.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            45205..46206
                     /gene="ltaE"
                     /locus_tag="ACHKUN_000245"
     CDS             45205..46206
                     /gene="ltaE"
                     /locus_tag="ACHKUN_000245"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO6658572.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            46217..47647
                     /gene="ybjT"
                     /locus_tag="ACHKUN_000246"
     CDS             46217..47647
                     /gene="ybjT"
                     /locus_tag="ACHKUN_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO6658573.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            47746..48759
                     /gene="ybjS"
                     /locus_tag="ACHKUN_000247"
     CDS             47746..48759
                     /gene="ybjS"
                     /locus_tag="ACHKUN_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6658574.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(48756..49586)
                     /gene="amiD"
                     /locus_tag="ACHKUN_000248"
     CDS             complement(48756..49586)
                     /gene="amiD"
                     /locus_tag="ACHKUN_000248"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO6658575.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(49583..49906)
                     /gene="ybjQ"
                     /locus_tag="ACHKUN_000249"
     CDS             complement(49583..49906)
                     /gene="ybjQ"
                     /locus_tag="ACHKUN_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO6658576.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            50032..50547
                     /gene="ybjP"
                     /locus_tag="ACHKUN_000250"
     CDS             50032..50547
                     /gene="ybjP"
                     /locus_tag="ACHKUN_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6658577.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            50765..51493
                     /gene="artP"
                     /locus_tag="ACHKUN_000251"
     CDS             50765..51493
                     /gene="artP"
                     /locus_tag="ACHKUN_000251"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO6658578.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            51511..52242
                     /gene="artI"
                     /locus_tag="ACHKUN_000252"
     CDS             51511..52242
                     /gene="artI"
                     /locus_tag="ACHKUN_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO6658579.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            52249..52965
                     /gene="artQ"
                     /locus_tag="ACHKUN_000253"
     CDS             52249..52965
                     /gene="artQ"
                     /locus_tag="ACHKUN_000253"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO6658580.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            52965..53633
                     /gene="artM"
                     /locus_tag="ACHKUN_000254"
     CDS             52965..53633
                     /gene="artM"
                     /locus_tag="ACHKUN_000254"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO6658581.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            53925..54656
                     /gene="artJ"
                     /locus_tag="ACHKUN_000255"
     CDS             53925..54656
                     /gene="artJ"
                     /locus_tag="ACHKUN_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO6658582.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(54831..55958)
                     /gene="rlmC"
                     /locus_tag="ACHKUN_000256"
     CDS             complement(54831..55958)
                     /gene="rlmC"
                     /locus_tag="ACHKUN_000256"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO6658583.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(55999..56487)
                     /gene="ybjO"
                     /locus_tag="ACHKUN_000257"
     CDS             complement(55999..56487)
                     /gene="ybjO"
                     /locus_tag="ACHKUN_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO6658584.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(56547..57392)
                     /gene="potI"
                     /locus_tag="ACHKUN_000258"
     CDS             complement(56547..57392)
                     /gene="potI"
                     /locus_tag="ACHKUN_000258"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO6658585.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(57389..58342)
                     /gene="potH"
                     /locus_tag="ACHKUN_000259"
     CDS             complement(57389..58342)
                     /gene="potH"
                     /locus_tag="ACHKUN_000259"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO6658586.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(58352..59485)
                     /gene="potG"
                     /locus_tag="ACHKUN_000260"
     CDS             complement(58352..59485)
                     /gene="potG"
                     /locus_tag="ACHKUN_000260"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO6658587.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(59580..60692)
                     /gene="potF"
                     /locus_tag="ACHKUN_000261"
     CDS             complement(59580..60692)
                     /gene="potF"
                     /locus_tag="ACHKUN_000261"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO6658588.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(61043..61519)
                     /gene="ybjN"
                     /locus_tag="ACHKUN_000262"
     CDS             complement(61043..61519)
                     /gene="ybjN"
                     /locus_tag="ACHKUN_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO6658589.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(61607..62509)
                     /gene="rimK"
                     /locus_tag="ACHKUN_000263"
     CDS             complement(61607..62509)
                     /gene="rimK"
                     /locus_tag="ACHKUN_000263"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO6658590.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(62570..63292)
                     /gene="nfsA"
                     /locus_tag="ACHKUN_000264"
     CDS             complement(62570..63292)
                     /gene="nfsA"
                     /locus_tag="ACHKUN_000264"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO6658591.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(63276..63563)
                     /gene="ybjC"
                     /locus_tag="ACHKUN_000265"
     CDS             complement(63276..63563)
                     /gene="ybjC"
                     /locus_tag="ACHKUN_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO6658592.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            63723..63980
                     /gene="grxA"
                     /locus_tag="ACHKUN_000266"
     CDS             63723..63980
                     /gene="grxA"
                     /locus_tag="ACHKUN_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO6658593.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(64010..64387)
                     /gene="ybjM"
                     /locus_tag="ACHKUN_000267"
     CDS             complement(64010..64387)
                     /gene="ybjM"
                     /locus_tag="ACHKUN_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO6658594.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
BASE COUNT        15618 a        17132 c        16060 g        15699 t
ORIGIN      
        1 gtcgccgact taataaagag agagttagtg tgaaagctga caaccctttt gatcttttac
       61 ttcctgctgc aatggccaaa gtggccgaag aggcgggtgt ctataaagca acgaaacatc
      121 cgcttaagac tttctatctg gcgattaccg ccggtgtttt catctcaatc gcattcgtct
      181 tctatatcac agcaaccact ggcacaggca caatgccctt cggcatggca aaactggttg
      241 gcggcatttg cttctctctg gggctgattc tttgtgttgt ctgcggagcc gatctcttta
      301 cttccacagt gttgattgtt gttgctaagg cgagtgggcg catcacctgg ggtcagttgg
      361 cgaaaaactg gctaaatgtc tattttggca acctggtcgg cgcactgctg tttgtacttt
      421 taatgtggct ttccggcgag tatatgaccg caaatggtca atggggacta aacgtcctac
      481 aaaccgccga ccacaaagtg caccatactt ttattgaggc cgtctgcctt ggtatcctgg
      541 caaacctgat ggtatgtctg gcagtatgga tgagttattc tggccgtagc ctgatggaca
      601 aagcgttcat tatggtgctg ccggtcgcga tgtttgttgc cagcggtttt gagcacagta
      661 tcgcaaacat gtttatgatc ccgatgggta ttgtaatccg cgacttcgca tctccggaat
      721 tctggaccgc tgtcggttct gcaccggaaa atttttctca cctgaccgtg atgaacttca
      781 tcactgataa cctgattccg gttacgatcg gtaatattat cggcggtggt ttgttggttg
      841 ggttgacata ctgggtcatt tacctgcgtg aaaacgatca ccattaatgg ttgtcgaagt
      901 acgcagtaaa taaaaaatcc acttaagaag gtaggtgtta catgtccgag cttaatgaaa
      961 agttagccac agcctgggaa ggttttacca aaggtgactg gcagaatgaa gtaaacgtcc
     1021 gtgacttcat tcagaaaaac tacactccgt acgagggtga cgagtccttc ctggctggcg
     1081 ctactgaagc gaccaccacc ctgtgggaca aagtaatgga aggcgttaaa ctggaaaacc
     1141 gcactcacgc gccagttgac tttgacaccg ctgttgcttc caccatcacc tctcacgacg
     1201 ctggctacat caacaagcag cttgagaaaa tcgttggtct gcagactgaa gctccgctga
     1261 aacgtgctct tatcccgttc ggtggtatca aaatgatcga aggttcctgc aaagcgtaca
     1321 accgcgaact ggatccgatg atcaaaaaaa tcttcactga ataccgtaaa actcacaacc
     1381 agggcgtgtt cgacgtttac actccggaca tcctgcgttg ccgtaaatct ggtgttctga
     1441 ccggtctgcc agatgcatat ggccgtggcc gtatcatcgg tgactaccgt cgcgttgcgc
     1501 tgtacggtat cgactacctg atgaaagaca aactggcaca gttcacttct ctgcaggctg
     1561 atctggaaaa cggcgtaaac ctggaacaga ctatccgtct gcgcgaagaa atcgctgaac
     1621 agcaccgcgc tctgggtcag atgaaagaaa tggctgcgaa atacggctac gacatctctg
     1681 gtccggctac caacgctcag gaagctatcc agtggactta cttcggctac ctggctgctg
     1741 ttaagtctca gaacggtgct gcaatgtcct tcggtcgtac ctccaccttc ctggatgtgt
     1801 acatcgaacg tgacctgaaa gctggcaaga tcaccgaaca agaagcgcag gaaatggttg
     1861 accacctggt catgaaactg cgtatggttc gcttcctgcg tactccggaa tacgatgaac
     1921 tgttctctgg cgacccgatc tgggcaaccg aatctatcgg tggtatgggc ctcgacggtc
     1981 gtaccctggt taccaaaaac agcttccgtt tcctgaacac cctgtacacc atgggtccgt
     2041 ctccggaacc gaacatgacc attctgtggt ctgaaaaact gccgctgaac ttcaagaaat
     2101 tcgccgctaa agtgtccatc gacacctctt ctctgcagta tgagaacgat gacctgatgc
     2161 gtccggactt caacaacgat gactacgcta ttgcttgctg cgtaagcccg atgatcgttg
     2221 gtaaacaaat gcagttcttc ggtgcgcgtg caaacctggc gaaaaccatg ctgtacgcaa
     2281 tcaacggcgg cgttgacgaa aaactgaaaa tgcaggttgg tccgaagtct gaaccgatca
     2341 aaggcgatgt cctgaactat gatgaagtga tggagcgcat ggatcacttc atggactggc
     2401 tggctaaaca gtacatcact gcactgaaca tcatccacta catgcacgac aagtacagct
     2461 acgaagcctc tctgatggcg ctgcacgacc gtgacgttat ccgcaccatg gcgtgtggta
     2521 tcgctggtct gtccgttgct gctgactccc tgtctgcaat caaatatgcg aaagttaaac
     2581 cgattcgtga cgaagacggt ctggctatcg acttcgaaat cgaaggcgaa tacccgcagt
     2641 ttggtaacaa tgatccgcgt gtagatgacc tggctgttga cctggtagaa cgtttcatga
     2701 agaaaattca gaaactgcac acctaccgtg acgctatccc gactcagtct gttctgacca
     2761 tcacttctaa cgttgtgtat ggtaagaaaa ctggtaacac cccagacggt cgtcgtgctg
     2821 gcgcgccgtt cggaccgggt gctaacccga tgcacggtcg tgaccagaaa ggtgctgtag
     2881 cgtctctgac ttccgttgct aaactgccgt ttgcttacgc taaagatggt atctcctaca
     2941 ccttctctat cgttccgaac gcactgggta aagacgacga agttcgtaag accaacctgg
     3001 ctggtctgat ggatggttac ttccaccacg aagcatccat cgaaggtggt cagcacctga
     3061 acgttaacgt gatgaaccgt gaaatgctgc tcgacgcgat ggaaaacccg gaaaaatatc
     3121 cgcagctgac catccgtgta tctggctacg cagtacgttt caactcgctg actaaagaac
     3181 agcagcagga cgttattact cgtaccttca ctcaatctat gtaattagat ttgactgaaa
     3241 tcgtacagta aaaagcatac aataaaggct ccacgaaagt ggggcctttt ttagcacgag
     3301 agcctttttt gtcagctatc tatactttaa ggtgactgcc aaaacagact cgacgtagcc
     3361 ttcgagctgc gcaccaacac ggcctcagat gggccacatc tggagaaaca ccgcaatgtc
     3421 agttattggt cgcattcact cctttgaatc ctgtggaacc gtagacggcc caggtattcg
     3481 ctttatcacc tttttccagg gctgcctgat gcgctgcctg tattgtcata accgcgacac
     3541 ctgggatacg catggcggta aagaagttac cgttgaagat ttaatgaaag aggtggtgac
     3601 ctatcgccac tttatgaacg cttccggcgg cggcgttacc gcatccggcg gtgaggcaat
     3661 cctacaagct gagtttgttc gtgactggtt ccgcgcctgc aaaaaagaag gcattcatac
     3721 ctgtctggac accaacggtt ttgttcgtcg ttacgatccg gtgattgatg aactgctgga
     3781 agtaaccgac ctggtaatgc tcgatctcaa acagatgaac gacgagatcc accaaaatct
     3841 ggttggagtt tccaaccacc gcacgctgga gttcgccaaa tatctggcga acaaaaatgt
     3901 gaaggtgtgg atccgctacg ttgttgtccc aggctggtct gacgatgacg attcagcgca
     3961 tcgcctcggt gaatttaccc gtgatatggg caacgttgag aaaatcgaac tcctccccta
     4021 ccacgagctg ggcaaacaca aatgggtggc aatgggtgaa gagtacaaac tcgacggtgt
     4081 taaaccacct aagaaagaga ccatggaacg cgtgaaaggc attcttgagc agtacggtca
     4141 taaggtaatg ttctaatttc cccgtaaagc ggcaacttta ttgagttgcc gcttttttat
     4201 tccgcgcttc attctgctac agcgttttta acacatttta tagtgcatct accatatccc
     4261 gtacctgaga taataacgac tgataatagc tcgcatgaag ttcttgccca tcgaatacca
     4321 ctgtattaca caagaaagtg acatcggcat cttcaatacc aagataactg cacatatttt
     4381 tcaaataatc gcttatattt ttttcccagc ccatctggac aaatgattct ttgtctcctc
     4441 caaccagcgc cacccaacga actttattga atggtaattt gtgcccatct ccataagcca
     4501 gcccattatt ccatactctg tcaatatatc cttttagcat tgccgggaag ctataccacc
     4561 agagaggaaa aaccaccact aacgtgtcat gttcaagcag ctctgaataa agctgatgaa
     4621 cctctggaga ataacgttta tccatattct tccagtctgg ttcatcttcc ggcgtcatca
     4681 ctgggttgaa attacgccga tataaatcga gttccgtcac ctgtattttc cgctccattg
     4741 cccgctgatg gatcgcatca gcaatatttg cggtcaatga atcatgacga ggatgggccc
     4801 ataccaaata aatacgttca gactgcataa ttttacctcc acgttaagtt ctggagcgca
     4861 ctttacgcag gcggcataat cctgtaaacg tgatgattca ggatgagtta atgaaggatt
     4921 ttcactaatg cggatgaata tgtctgactt tgccactttc tttgccgtcg cccgtaatca
     4981 aagctttcgt gcagcgggcg atgagttagg cttatcctcg tccgccatta gccatagtat
     5041 taaaacactg gaacaacgtc ttaaaattcg cttattcaat cggacaaccc gaagcgtatc
     5101 gttaacggaa gcgggttcta atctctatga acgattgcgc ccagctttcg acgaaattca
     5161 gatcatgtta gatgagatga atgattttcg tctgacaccg acaggaacgc tcaaaatcaa
     5221 tgccgcccgg gtagctgcac gtatattttt aatgccttta ctggttggtt tcacacgtga
     5281 atatcctgat attaaagtcg agctcacaac tgacgattct cttgtcgata tcgtccaaca
     5341 gggctttgac gctggcgttc gacttagtgg catcgtcgaa aaagatatga tcagcgtggc
     5401 gatcggccca ccagtgaaat tatgtgtagc agcaacgcca gagtactttg caagatatgg
     5461 caaaccccgc catcctcatg atctgcttaa tcatcaatgt gttgtttttc gttatcccag
     5521 cggcaaacct ttccactggc aatttgcaaa ggagctggaa attgctgtcg caggaaatat
     5581 cattctggat gatgttgatg ctgaactgga ggccgttttg atgggtgctg gtatcgggta
     5641 tctgctctat gaacagatca aggagtacct tgatactggt cgtttagaat gtgtactggg
     5701 agactggagc acagagcgtc caggcttcca gatctattat ccaaaccgcc agtatatgtc
     5761 ctgcggcttg cgcgcatttc tcgattatgt aaaaacgggt cagatctgcc agagtcagcg
     5821 tcaccgacca caataattat ttactattcg ctttacgcgc cagcagcggg aaaatcagtc
     5881 ctaatcctac cagcacaaac ggcgttgcaa cattcagcgc caactggaag gtccactctg
     5941 cagtgaaggc ttccattttc gggaagatcc ccgtcaaaca ggcaaaggcg gtaaaggcaa
     6001 aacaccaaat cccgacggtc attgccagag gcttattacg aataaagaca tattctggtt
     6061 tatatttctg cgccaggcga acgactgcaa taaatgcaac aaatacccat aaataacgca
     6121 gcggcatcac ttaacgggcg taccagaagc gttggtacga cataaactgg ctggaatata
     6181 tttgctgtca gcatcaccta atagcacttt aagcggggca tcaatcgaga ataccagcgc
     6241 agctacttgt cccagggtat tcgcaatggc gtaaatcacc attaaagtat tacccatgtt
     6301 gtaatactcg cccagcttct gaaaggcgta atattgaccg ttagtcatta agtcatccgg
     6361 gatattacgc gagtcaaaca tcatccccat cgccagtgag cccagaatgg cacaaaccgc
     6421 aaccatcacc gccaggcata gcatcccttt tggaaattct tttcctgggt tgcgcgtttg
     6481 attaacgtaa ggagaaatct tctctgctcc gccaaccgcg aaaaccagca ttgaaatagt
     6541 ggtaatgtag gtaaagtcga tatgaggaat gaacgtttcc caggtaatgt ttgtggtcgc
     6601 aatatgcact tcagtaatcg cagacgcggt taccgccatc gccacataca ggagcgacat
     6661 aacaaacatc gcaatccctg ccacagaacc gacgattttc agcgatttca taccgcgtga
     6721 agcaacccac ataaagaaga taaacagcac cagcgttaac ccctgtaacg ctacgactga
     6781 atattctttg attagcgaac cgtcgccttt catcgcccaa ccgagcgcaa tcagaattgc
     6841 ctggggtttt tgtgccagaa agggaatatg caccacccag taggtccacg cggcgagata
     6901 agccagtccg ggtcccatcg tgtgtttaat ccaggtactg accccgccct tcccatcttt
     6961 gaaggtcgag cctaactgac caacaattag cgcataaggt gtgaaataga gtgcaaagat
     7021 aaacacccat gaaaaaacaa ccaccagccc ctggttggca tagttattaa caacgttgcc
     7081 aaaaccccag acagtgataa aagacatcag cgcaatgttg taccatcgca actgtttttc
     7141 ctgaacattc ccagccatag acaaatcctt cgccaaaatt aaaggggcat ctgtaaaacg
     7201 agggattatg cgcggcaaat aagaagtgcg gaaatagcga aatgcaaaat tataacctgc
     7261 tgcacatttt atttttaaac gaataaaatt tggaataaag caagtcagat agaagaaaaa
     7321 aaaagcagag tgtgaaacag taagttcaca ctctgcttga attcatttac acgtgagcaa
     7381 cgggtttcgg cgtatgaccg gcgttgcgca gcagcatcag caaatagata aacgatacgc
     7441 tggcgatcat gataaacaat aaattatcgg agaaattctg cattagcata gcggtaaatg
     7501 acgggccaag cagacttccc acagtatagc tcaacagtaa ggcctggttc atcgccacca
     7561 gttgatgatg ttcaactttc tcgcaagccc atgccatcgc caccggatat agcgtaaagc
     7621 cagcggcacc gaggatgaat aacgctgggg ccatcgccgc ctggctaagc atcgcgatac
     7681 tgccgagaat gacgacaaag acctgaacac gcaacaccag cagtcgacca aacttatccg
     7741 ccagacgtcc aatcggccat tgtccaagga tacccgcact gaccagtacc gccatccaga
     7801 aaccaatgct ggcattgctc acccctttgt gattgaggta cagcggcatc aggccatata
     7861 gagatcccag aacgattcct gagataatgc agccattcac gccaagccgc gcctgacgga
     7921 gttttagcat tgacgtaatt gacgtcgaat catggttttc agcctgctga ttcagcacac
     7981 gcgtaaacaa cagcggtaag atccctgcca acgtcaaacc tgtaacccac ggcaatacgg
     8041 acatcagctc ggttgaaact ttgctgacca gtaactggcc taaaaacgtt cccacgtaat
     8101 aaaccatcat atacgcagca agcaaacgcc cacggttacg tgacgtccca ctgcacatca
     8161 gcgcgctctc aacaaccacc caaatcatgg cacagccgac gcccgcgaca aaacgccaag
     8221 ccaaccagct ccagaatcca atcatcaggc caaggccggc acagccagcg gcaaaaatga
     8281 aggaggccag ataatagctg cggttaaagc caatgcgctt aatgacatac cctgtcagca
     8341 atgtaccgac aaggttgccg gtaaaatagg atgagctgac aacgcctacc tgccatgtgg
     8401 acatgtgttc ctgggcgagc caaagcggca cgagtgtatt taacaccgcg atcgccagag
     8461 tcaacaaaag caggccagac agcaaaagca tgacaggccg ggtatacgtg gacatgaatt
     8521 aaaaccgtga ggaagttcaa atttcatgcg catcatgcca ccgccaaaaa cattgtcaat
     8581 gggtgaaaat acgcgcatat cgcagcggcg gtattatcga tttatattac tcattcataa
     8641 cgctaatgct gaagagatga ttaatctcat ctgattgttt ttattgatct aacacaaatt
     8701 tcatacgctt cgttcagtcg aaaaatttca tggtcacagc aaactcatat ttcccgtcta
     8761 tgctttcaga gcaatgcatc acgtctgacc gatttcagac gtaccactga ctctcgaaag
     8821 gagaagagga tgaccaaacc gtatgttcgt cttgataaaa atgatgctgc cgttttgctt
     8881 gttgatcatc aggctggttt actttccctt gtacgggata tcgaacccga taagtttaaa
     8941 aataacgtgc tggcgctggg tgacctggct aagtatttca acttacctac tattcttacc
     9001 accagttttg aaaccggtcc taacggtcca ttagttccag aactgaaagc acaattcccc
     9061 gatgcacctt acattgctcg cccgggaaat attaacgcct gggataacga agattttgta
     9121 aaagcagtca aagcgacagg taaaaaacag ttaattattg ccggtgtggt aaccgaagtt
     9181 tgcgtggcat tcccggcgct ttcagccatt gaagaagggt ttgatgtctt tgttgtgact
     9241 gacgcgtcag ggacctttaa tgaaattacc cggcattcgg catgggatcg catgtcgcaa
     9301 gcaggtgcgc aattgatgac atggttcggc gtggcgtgcg agctgcaccg tgactggcgt
     9361 aatgatattg aaggactggc gacgttgttc tctaaccata ttcctgatta tcgtaatttg
     9421 atgaccagtt atgacacgtt aacgaagcag aaataaaaga gaaccggggc cgaaaccccg
     9481 gttgttactg attagcttgc gacggccatc cccacggtca tatgcaaacc atagaatacg
     9541 ccgcgaccga ttaattcccc tgccagaagc agaatgaatg agacggatag tagcggaacc
     9601 gcaggctgat aaccttttag ctgtggtgca atccacaagc acagggcaac ggccaaaagc
     9661 acgatccgcc aggacatcag cgcaccatag tccggcacca gcgctgcggc ctgctgcaca
     9721 gaactatgaa tagttgccag ctctgtgccc tgcatcactg acatcacgcc actcaccacc
     9781 agtgccagta cagaaatggc tggcagcaga cgcatcgccc agccatcgac gccagccaga
     9841 ctcaacaaca ggtaacccag taacgggccg cccataaaca tcgtcaggaa gaagcccatc
     9901 ggcgtccaga tgctgtacca ggtcggaacg gtatcaatgc tgttatacac acgcaccatc
     9961 atccagacaa agatgacgcc aagaaccatc gtcactatca gccacagcgt acgcaatgcc
    10021 ggtgacagct ttttcagcat tgccagcaac cagccgatgc cgcctacggc aaaaaagatc
    10081 gaaccgctgg cgatttcgtt actgagtgct gaagccccta cccggttgag cgagttaaaa
    10141 gcgcgcattg gtgaaccaag atggagcata gaggcgataa agccaatgcc catcagcacc
    10201 cataaaccaa acatgcaggc gataacacgc tgctgggctt ctgctcgcag gtcgcctttg
    10261 agcagcgcca aagccagaac gataaaacca cctgctacac attgcccgaa gaccgtgaag
    10321 atcatcagcg gccattcatg ccatccactt cccatctcac acctccttcg ggtttgccag
    10381 atagccagtg gtatccccgg tcgggcggct attggcattg ggtttgatca caatattcgg
    10441 tttggtaaag tgagctcgcg gcaacggcgc aacggccgcc agatcgccat gttttttacg
    10501 cagctcgtcg atagggccga aatccagcgc ccgcagcgga caagattcaa cacagatcgg
    10561 ctttttaccc tccgcaacac ggtcataaca accatcgcat ttggtcatat ggcctttcgt
    10621 ttcgttatat tgcggtgcgc catacgggca agccatatgg cagtagcggc agccaatgca
    10681 cacatcttca tcgaccacaa caaaaccatc ttcacgttta tgcatcgcac cgctcgggca
    10741 gactttagta caagccggat cttcgcagtg gttacatgaa atcgacagat agtaggcaaa
    10801 cacgttctgg tgccagacac cgttatcttc ctgccagtcg ccgccagcat attcataaat
    10861 gcggcggaag ctgacttctg gcgtcaaatc tttgtagtct ttacaggcca gctcgcaggt
    10921 tttgcaaccg gtgcaacggc tggaatcaat aaaaaatcca tactgggttg tcatcggtta
    10981 ctccttacac cttttcaacc tgaacaaggt ttgtatgtga cggattcccc ttagcgagag
    11041 gagacggacg ttgagtggtc agtacgttga tacaaccgcc cttatcgaca cgttttgtat
    11101 ccgggtcata ccaggcacct tcacccagtg cgaccacacc cggcatcatt cgtggcgtca
    11161 ctttcgcctc aatatgaacc tcaccacgat cgttaaagat cctgacttta tcgccgttgt
    11221 tgataccgcg tttttgcgca tcaagcgggt tgatccacat ttcctgacgg caagctgctt
    11281 tcagcacatc aacgttgccg taagttgagt gaacgcgaga tttatagtgg aaacccgtaa
    11341 gctgcagcgg atactgtttg ttcagcggat cctgataact ttcaaagccc ggcgtgtaga
    11401 tcggcagtgg atcgatcaca tcgccttctg gcaattccca ggtagcggca atgtcagcca
    11461 gcgcctgcga ataaatctca attttgcccg atggcgtagt cagtgggttt gcctgcggat
    11521 cttcacggaa ggctttataa gcaacgtgat gcccttgtgg gtcgcgcttt ttaaagatcc
    11581 cctgcttgcg gaactcttca aacgttggca gttcaggaat cgcttcccgc gactgggcat
    11641 acagatgccg catccactct tcctgggtac ggccttcagt aaactgttgc tcaacgccaa
    11701 gacgttttgc cagttcgctg gtcatttcat agatggtctt acattcaaag cgcggtttaa
    11761 tcacctgatc gttgaaaatc acgtaagaca tattcccgca ggatgcatcc agtgcaaagt
    11821 ccatctgttc ggaagcggtg cagtcaggca gcaggatgtc agcatatttc gccgatgagg
    11881 tcatgtggca gtcgataacc acaatcagct cgcacttctt atcatcctga aggatttcat
    11941 gggtacggtt gatttcagaa tgctggttaa tcaggcagtt accggcatag ttccagatca
    12001 ttttgatcgg cacatccagc ttatctttcc cgcgtacacc atcacgcagc gccgtcattt
    12061 ccgggccacg ttcaatggca tcggtccaca taaacatcga aatgctggtc tggatcgggt
    12121 tttccaaggt cggcatacgg acaaacggta agctgtatga accttcacgc gcgccgctgt
    12181 tgcctccgtt aataccaacg ttaccggtca gaatcgccag catcgagata gcacgggttg
    12241 cgatttcacc gttagcgtga cgctgcgggc cccatccctg gctgataaac gccggtttgg
    12301 tactgccgat ttcacgagcc agtttgatga ttttgtctgc cggaacaccg gtgatttgtg
    12361 aggcccattc cggtgtttta gccacgccat ctggcccttc acccagaata taagctttat
    12421 agtggccatt tttcggcgca ctggctggca gggttttctc atcgtagcca acgcaatatt
    12481 tatcgaggaa tgcctgatcc accaggtttt cagtgatcat gacgtacgcc agaccgttaa
    12541 ccagtgcggc atctgttccc ggacgaatag ggatccactc atcttcgcgc ccggcaccgg
    12601 tgtcggtata gcgcggatca atgatgatca tgcgggcatt agatttctgg cgtgcctgtt
    12661 caagatagta agtcacccca ccgccactca ttcgcgtttc gccagggtta ttaccaaaca
    12721 gcactaccag cttactgttt tcgatatccg acgggctgtt gccatctgcc cagccgccgt
    12781 aggtatagtt caaaccttcc gcaatttgcg cggaggagta gtcgccgtaa tggttcagat
    12841 agccgccgca gcagttcatc agccgcgcga ccagggtatt tcccggcggc caggagcggg
    12901 tcatggtgcc gcccagcgta ccggtgccat agttcagata gatagactcg ttgccgtact
    12961 ctttgatcag gcgctgcata ttggtcgcga tgatgtcgta ggcttcttcc cagctaatgc
    13021 gctcgaattt gccttcaccg cgcgccccga ctcgtttcat cggatatttc aggcggtccg
    13081 gattgtagac acggcgacgc atggaacgcc cacgcaggca ggcgcgaacc tggtgcaggc
    13141 cgtcgtaatt gtcatcgccg gtattgtccg tttcgacata tttgatttca ccgtccacga
    13201 cgtgcatacg tagcgggcag cgactaccac agttaactgt acaggcgctc cagataacct
    13261 tttcgtctga ttttgttgga atggcgctat cgacagcgtg cgcaatccga ctaaaaggta
    13321 atgttaatgc gctgctggcc attgccaggc cgccgatcgc tgtcgttttt accaaaccac
    13381 ggcgactcac ctcagcagcc aataccgcat cagggatttt cgttttcata atggctcact
    13441 caagcttgct cacgattgat tccagtaaca aaccgtattg tgtaattatt tctatagcag
    13501 ctcgctatat aggcttgtat acatcgaata atttgaaaat caaagagata ggtagatttt
    13561 cactcgaaag tagtattacg tgtaagtgag gagtaattat tgttcggtat caaagggaga
    13621 caaaaaaagc gcccgcaggc gctttttaga ttcagaaaaa ttgggtatta gccaatatat
    13681 tccagtccgt tcatatacgg acgcagaact tctggtactt caatacgacc atcagcctgc
    13741 tgatagtttt ccattactgc aaccagcgta cgaccaacag ccagtccaga accgttcagg
    13801 gtatgaacca gacgggtttt cttgtccgac ttgctgcggc agcgtgcctg catacgacgc
    13861 gcctggaaat cccaaacgtt ggagcaggaa gagatctcac ggtaggtgtt ctgtgccggg
    13921 atccatactt ccaggtcgta agttttgcaa gcgccaaagc ccatgtcgcc agtgcaaagg
    13981 atgattttac ggtacggcag gcccaacaac tgcaggactt tttctgcatg accagtcatc
    14041 tcttccagcg ccgccattga gtcttccggg cgcacgatct gcaccatttc aactttgtcg
    14101 aactggtgca tacggatcag accacgggtg tcacgaccat atgaaccggc ttcagaacgg
    14161 aagcatgggg tgtgggcggt catcttaatt ggcagatcat cttcatcgat gatttcaccg
    14221 cgcaccaggt tagtcagcgg aacttctgcc gttgggatca gcgcatagtt actggtgtct
    14281 gcttcttctt ccagcggacg agtatgaaac agatcgccag caaatttcgg cagctgaccc
    14341 gtaccgtaca gcgtgtcctg gttaaccagg tacggaacat agttctcact gtagccatgc
    14401 tgttcggtat gcagatccag cataaactgc gacagtgcgc ggtgcatgcg agcaatctgc
    14461 cctttcatta ccacaaagcg ggaaccagtc agcttaactg cagctgcaaa gtcgaggcca
    14521 gagtgcattt cacccagcgt cacatggtca cgaacttcaa agtcaaactc acgcggggtg
    14581 ccccagcggc tgacttcgac gttgtcattt tcgtctttac ctaccggcac ttcatctgca
    14641 ggcaggttag ggatggtcag cgcgatatcg cgaatttcag cctgtaaagc atccagctcg
    14701 gcttttgctg catccagctc ttcgcccagt ttgttcactt ccagacgtaa aggctcgata
    14761 tcttccccgc gcgctttcgc ctggccaatg gatttcgatc gggagttacg ctccgcttgc
    14821 aggttttccg ttttgacctg caatacttta cgacgctctt caagagcgcc cagcttatct
    14881 acatccagct taaagccccg gcgtgccagt ttttcagcga ctgcgtctgg ctcattacgc
    14941 agcagattgg gatcgagcat gcttatcctg tgcttatcga attaaatggg agatgtggcc
    15001 acagcctgcg accacaggga tacagtaaca acattaccgc aacgataaca ttaacggtag
    15061 cgttttatgg ggctattttg atcctgttca gccagccagg cgagcttttc gccaatcttg
    15121 ccttcaaggc ccctgtttgt cgggaaataa tagcgtgttt gtgctatttc cggcgggaag
    15181 taaacctcac cggcagcata agcgtttgct tcatcatgag cgtaacgata ttcctgcccg
    15241 tagcccattt ccttcattaa tttcgtcggc gcattacgca aatgaaccgg cacgtcataa
    15301 tccggacgtt cgcgagcatc cgccagcgca gctttaaacg cagtgtagac agcgttgctt
    15361 tttggcgcgc aggccaggta aacaatcgcc tgagcaatgg cgcgttcacc ttccgccggg
    15421 ccaacgcgag taaagcaatc ccaggccgca attgccacct gcatcgcccg tgggtcggca
    15481 ttgccgacat cttcggaagc aattgccagg caacgacgcg cgacatataa cggatcgcca
    15541 ccagcggtaa taattcgcgc ataccagtag agcgccgcat cgggtgcgct accacgtacc
    15601 gacttatgca gtgcggaaat gagatcgtaa aagcgatcgc ctttgttatc aaagcgggcg
    15661 cttcgctcac cggcgatttc ggtcaataat tcaggcttca ggacccgctt accgctatca
    15721 ttgacttcgg ccatatccgc catcatttcc agcgtattta acgcccggcg cgcgtcgcca
    15781 ttcaccagtt cagcaatggc gcgtcgtgtt tcatctggca gaacaatatc ctgaccacca
    15841 taaccacggg tcttgtcttc catcgcctga gttagtactt gctcaatatc ctctgtaccc
    15901 agggatttca acagatagac acgggcacgg gaaagcagtg ccgaattaag ctcaaacgac
    15961 gggttttcag tggttgcgcc aataaaagtg atggtgccgt cttcaatatg tggcagaaat
    16021 gcatcctgct ggcttttgtt gaatcggtgc acttcgtcaa caaaaagaat agtgcggcga
    16081 cctgcattgc ggttttgccg ggcgcgctcg atcgcctcgc gaatctcttt cacgccagag
    16141 gtgacggcag aaatacgttc cacatcagcg ttcgcatagc gggcaatcac ttcagcgagg
    16201 gttgttttgc cggtacccgg tggcccccag aggatcatag aatgtaaatg cccggcttcg
    16261 atagcgcgcg gcaacggctt ccccgcagcc agcaaatgtt gctggccgat atactgtgct
    16321 aaattttctg gccgcatacg cgcggccaga ggttgaaaag tattatccga aaaatcgagc
    16381 gacagattgc tcactcaggt gcctctactt acgttgatca tctaccgtga cgccttgcgg
    16441 cggggtgaag gtaaatttcg ctgcatccac agccccattt tgctgggatt tcagttgata
    16501 actgctgcgc tgatcgtcct gctccaccgc gctaaactga tggattgtgc catcacgtcc
    16561 cacgttaatg gtgaactgct tcagattgcc attgctggct ttcggcgtca ggacaaagtc
    16621 atcgccattc tgtttgatat tgtactgctg ccagtcgctg gactggttgc gggcaatcag
    16681 cataaacggc gtattaccgg tggcatcttt cagccaggtt gccgtagctt gctcaacgaa
    16741 cgggttatag aaccacagtg ttttaccgtc agaaaccaga atgctttcat caggttgtgt
    16801 catatgccag ttgaataagt ttggacgttt cacccacaga tcgccctgac cttcctgcac
    16861 cgccgcgccg ctaccgtcag tcactttttg tgtgaagctg gcgtggaagc tgctgacttt
    16921 atccaggcgg cttttcagat cgcttgcggc atcagcccaa acgctgcttg ctactaagct
    16981 tgagagtaat gcacaggtga tggcaatttt tttcatcatt attcctcaaa ttacgtcact
    17041 cccgatcggg agttccctct actctctatt ccaggctaat aatcagcttg aggaaagaag
    17101 aaaatactga attttcagtt gatagagtgc cggatgcgac gctaccgcgc cttatccggc
    17161 aatgcattaa ttagtcaaac ggcggtgggg ccagcacttc acgattaccg ttgtgcccct
    17221 gttcgctgac aatcccctgc gcttccatct gttcgataat acgcgctgca cggttataac
    17281 caatgcggaa ctgacgctgt acgccagaaa ttgacgcttt gcgtttttca gtgacaaact
    17341 gcaccgcctg atcgaacaac ggatccagtt cttcagcgcc atcgaaaccg cccgcgccac
    17401 cttcgctttc actgtcggaa gtgatgccat caacatactg tgggcgacca cgcgctttcc
    17461 agtcctgcac cacggcatga acttcctgat cgcgaacaaa agcaccatgg acacgtaccg
    17521 gcaacgtgga gttcggccca gagtagagca tatcccccat ccccagcagt gattccgcgc
    17581 cagcctgatc aagaatggta cgtgagtcaa tcttactgga tacggtaaag gcgatacggg
    17641 tcggaatatt cgctttaatc agaccagtaa taacatcaac cgatggacgc tgagttgcca
    17701 gtacgaggtg gatacccgcg gcacgggctt tttgcgccag acgtgctatc agctcttcca
    17761 cttttttacc taccgtcatc atcaggtcgg caaattcgtc aaccaacacc acaatgtatg
    17821 gttctttttt cagcaccgga tgctgggcat ccatactgtc acccggcttc cagtacgggt
    17881 ctggaatcgg acgcatcatg cgatcggctt cagcaatttt ttcgttataa cccgccagat
    17941 tacgcacacc cagcgcagac atcagtttat accgacgctc catctcgtta acacaccagc
    18001 gcagcgcgtt ggcggcatct ttcatatcag taacgacttc cgttaacaga tgcggaatgc
    18061 cttcataaac cgaaagctcc agcattttcg ggtcgatcat gatgaaacgc acatcttctg
    18121 gctgtgcttt ataaagcatg ctcaggatca tcgcgttcac accgacagat ttaccggaac
    18181 cggtagtccc cgcaaccaac aagtgcggca ttttcgccag atcggcaacc accggctcac
    18241 cggcgatatc tttacccagc accacggtta atggcgacgg attatcgcgg aatttggcgt
    18301 tatccaaaac ttcgcgcaga taaacggttt gtcgtttttt atttggtaac tccagaccta
    18361 catagggttt gccaggaata acttcaacga cacgcaccgc caccgtcgaa agtgaacggg
    18421 caaggtcccg tgacaagtta gaaatgcgcg ccgcttttac gccaggagcc aggttaagtt
    18481 caaagcgagt gataaccggc cccggagagt aattgacgac atcggcttta atacggaaat
    18541 cagccagacg cgcttccacc aggcgagcca tttgttcaag cgcaaaggta tctaccggct
    18601 ccacttcgct cggcggcggt gtcagcaaat ccagagaagg cagcggcgtc gtcggtttat
    18661 gcaacggacg gctgtcgcca ttacgcatca acagcggatg aagcagggta tcctgctgct
    18721 gcggtgcaac cggttgttgc ggctgttgat actgcggctg cggagcaact ggttgttgcg
    18781 gctgctgata ttgcggctgt ggcgcaaccg gttgttgcgg ctgctgatat tgtggctgcg
    18841 gcgcaacctg ctgttgtggc tgctgatact gcggctgcgg cgcaaccggc tgttgcggct
    18901 gctgatattg ctgctgtggc ggaactggtt gttgcggctg ctgatactgc tgctgcggtg
    18961 caaccggttg ttgcggctgc tgtacaggtt caacaattgg cgtaaacaac ggttcgtgtg
    19021 gaccatcatc cagcaacgct ttcattggcg aaaattcaaa atcatccagc gagaacggat
    19081 tcgccccagc cggttgttcg ccggaataac gttgttgttg agtttgcgca aactgacgag
    19141 ccagttcagc ctctgccgca gcatctgcat cttctgcgtt tacgggcaca tcatgttgat
    19201 actgttcgcc atagcgttgc tgctgtgtct gggcgaactg acgtgccagt tcatcctgct
    19261 gcatcgcatc gatttcatca tcgttatact gatcgccaga atcatactga ttgcgctggg
    19321 cttcacgggc tttctcttcc gccgcacgct gtgagggcag cttaataccg taagacgcca
    19381 gttcacgacg agttggcacg cggatacgtt tcggtcgtgg caactgcggg ccaatcccct
    19441 ctttgacttg cggacgcggt ccaccgctat ttgccagact gaagactggc gcggcaacgg
    19501 ttgcggcagc ccccgtcgcc agtgtcgctt ttttcacgcc agatgccagc ggagaaacag
    19561 cggcagcggc ttctactgga ggtactgctg caacagaagg cgctttcaac gaagatttga
    19621 tcggttctgg ttctttaacc ggttctggaa tcggttgata ccaggccgca agttgttcac
    19681 gttcacgggc tcgcttctct tccacttctt caaaatagta aagcggcgga cgcgcgggtt
    19741 ttgtctcttc tacaacgggt tcaggctcca caacaggctg ctgttcaacg gattgcggct
    19801 gttggtacaa cggctcctga gcggctggtt gctgataagt ttgctcagtc tggtatgtag
    19861 actgtggagc aaaagtggat tgctgctctt cggcttgcca ggcgttacct gccaccggtt
    19921 gttctggcgc aggggcgtaa tacggctgtt gtgcaggctg ttccggcgca gttgcgtaat
    19981 acggttgctg aacaggttgt tccgccgcag gagcgtagta aggttgttgc gcaggttgtt
    20041 cagctgcagg tgcataatac gactgctgcg gctgtactgg ttgttgcagc ggctcattat
    20101 attgcactgc aggctgcgca tattgtgact gctgtgggta accttccggt gcaggagcaa
    20161 taaccggctc tcccgtttgt ggacccggta caggctgcca ggctactgta ggttgcgagg
    20221 gtggaacatc aacagaggca acaggcggcg tctgagtcac aggttcaacc ggcgcagccc
    20281 agctttgtgt cgccgtggta gcagcagctg ctacagcgac aggttcggta attggcgcac
    20341 cgtttaataa tggatcgtat tcgtcatatt ctggctgcgt tgcacgattg cccgaaaata
    20401 ggacgtcgtc cgggtcggca gccacaccgc gtgcagtgta ggtaatctct tcgtcatcat
    20461 ccatccgctt accggagaac aacgcagcgt ctgtttgccg ccccatcgga ttaatgaatt
    20521 tttccgccaa ccgtttacga cgcgctagcg cgccgcgaag aatacgggca cggcgtgatt
    20581 catgctgttt gccgtggttt tcatcttcat actcttcgtc atcttcatat tcatcttcat
    20641 cgacccaggt atcatcgcga cgggtacgat tactggcgaa ggtgagaatg tttaaaatcc
    20701 agccgccgag tttttcagca atggtcaccc atgaccaacc ggtgaacagc gtcaggcccg
    20761 cagcccaaac gcagagcagc gcaatagttc ccccgctact gtgtagcagt ggttgtagcg
    20821 tagtacttag taagctgcca atgacgccac cggaggcaaa ataccagata tcgtcagcgt
    20881 tgattgccgc cagaccacag gaggtaagga tgagcgccaa aacgccaatg atgcgtagcg
    20941 aaacggcaaa ataatcaatg tattcgtcgc tggactgatg acgccaggca aaccaacaac
    21001 cgccgacaat aatgacggga atggtgtaag ccatcacgcc aaaaataaag aacagcgtat
    21061 ctgccaacca cgcaccgggc atcccaccta aattatggat aggttcatgc caggccgttt
    21121 gcgaccagct ggggtccgaa gggttaaagc ttagtaaggc agccatcaac cagacggcaa
    21181 acaggacaat aaggatcagc aacgcctcca gaaggcggcg gccgctactt aactttgtca
    21241 atgtgacttc tttgtcttca gtgtattcct ggctcaagaa aggctctcca ggttacaggc
    21301 ttttcctgcc cgatgctaaa acggacaaca gtaccaggtt accccagtac tgttgctgta
    21361 tggattaaca ggagtgtaat caaaatacgc cgattttgca cctgttccgt gttagcgcgt
    21421 cttaataacc agacgattac tctgcttgac ttcttccata acaacgtatg tccgtgtgtc
    21481 attgacgcca ggcagacgca gcagggtttc ccccagcaac ttacggtaag ctgacatatc
    21541 cggcacgcgt gttttcaaca ggtagtcgaa atcaccagat actaaatgac actcctgaat
    21601 ttcttcaagt ttttgtacag cggtattgaa ttgttcaaac acatccggtg cgccacgatt
    21661 cagagtaatc tcaacgaata ccagaagtga tgcatccaga taatgggggt tcagcagcgc
    21721 cgtatagccc tgaataaacc cttgtctttc cagccgacgc acacgctcaa ggcacggcgt
    21781 tggggaaagt cccacacgtt tagaaagctc gacgttagaa atacgcccat ccttttgcaa
    21841 ctcattaaga atgttacgat cgatacggtc gagatctttg ccagggcgct tcttgctatc
    21901 taccattatt attgtctctc tgtattcctt ccctactcct gtctgaccct gaaacatcac
    21961 tgttcagagt cgctacccgt cacgatagac cgaaatcatg aagattcacg gcgcgcaatg
    22021 cctgggtcta cggtgagaag accgttgcct tgtggcagct atcgacatca cgaatggcgt
    22081 ctgtccacgc catacgtaga tttcactagc acggtaaaca tggtatttac atgcatggtt
    22141 atgcgcatat aagaatactg tttttttctt cctcgaatgt tttcgcaaaa caccagggga
    22201 ttgtcaaagc aaaacatcga tttttagtac aacatgccag atattcatta acaggggcgt
    22261 ttattcatca tttaatcggc ttataaagcg ggaaatttaa gtagcagagg tgataatgac
    22321 gggagaattt gacctgctta tcgatgctgc cgattgcaca aattgttaac aaaatcgtca
    22381 tactcttttt ttacgtctgt aaattcccta caatcctgcc cattgtctgc caacaactat
    22441 ggggatctca tgggcacgac caaacacagt aaactgctta tcctgggttc aggcccggcg
    22501 ggatacaccg ctgctgtcta cgcggcgcgc gccaacctgc aacctgtgct gattaccggc
    22561 atggaaaaag gcggccaact gaccaccacc acggaagtgg aaaactggcc tggcgatcca
    22621 aacgatctga ccggtccgtt attaatggag cgcatgcacg aacatgccac caagtttgaa
    22681 actgagatca tttttgatca tatcaacaag gtggatctgc aaaaccgtcc gttccgtctg
    22741 aatggcgata acggcgaata cacttgcgac gcgctgatta ttgccaccgg agcttctgca
    22801 cgctatctcg gcctgccctc tgaagaagcc tttaaaggca gtggggtttc tgcttgtgca
    22861 acctgcgacg gtttcttcta tcgcaaccag aaagttgcgg tcatcggcgg cggcaatacc
    22921 gcggttgaag aggcgctgta tctgtctaac atcgcttcgg aagtgcatct tattcaccgc
    22981 cgtgacggtt tccgcgcgga aaaaatcctt attaagcgtc tgatggataa agtggagaac
    23041 ggcaacatca ttctgcacac caaccgtacg ctggaagaag tgaccggcga tcaaatgggt
    23101 gtcactggcg ttcgtctgcg cgatacgcaa aacagcgata acatcgagtc actcgacgtt
    23161 gctggtctgt ttgttgctat cggtcacagc ccgaatactg ccattttcga agggcagctg
    23221 gaactggaaa acggctacat caaagtgcag tcgggtattc atggtaatgc cacccagacc
    23281 agcattcctg gcgtctttgc cgcgggtgac gtgatggatc atatttatcg tcaggccatt
    23341 acttcggccg gtacaggctg catggcagca cttgatgcgg aacgctacct cgatggttta
    23401 gctgacgcaa aataatttta caaatcagta acaaaagtaa agaaggcgac accatgcgac
    23461 tatgggtcgc ctttattttt tccccgttgt aacattgctc tgcaaataat tctgataact
    23521 cacctgctaa gcgtgcaatg aataaatccc gtcaaaaaga gttaacccgc tggttaaaac
    23581 agcaaagcgt catctcccaa cgttggctga atatttctcg tctgctgggc tttgtaagcg
    23641 gcatattgat cattgcccag gcctggttca tggcgcgaat tctgcaacat atgattatgg
    23701 agaatattcc ccgtgaagcc ctgctgcttc cctttacgtt actggttctg acctttgtac
    23761 tgcgcgcatg ggtggtctgg ttacgcgaac gggtgggtta tcacgccggg cagcatatcc
    23821 gctttgccat ccgccgtcag gttctcgacc gtctgcaaca agcagggcca gcgtggattc
    23881 agggtaaacc tgcggggagc tgggcgacgt tggtactcga gcaaattgac gatatgcatg
    23941 attactatgc acgctacctg ccgcaaatgg cgctggcagt gtcggtgccg ttgctgattg
    24001 tggtggcgat cttcccctct aactgggctg cggcgctcat tctgctgggc acagcaccgt
    24061 taattccgtt gtttatggcg ctggttggga tgggggctgc cgatgctaac cggcgtaact
    24121 ttctcgctct tgctcgctta agtgggcatt tcctcgatcg cctgcgcggc atggaaacat
    24181 tgcgtatttt tggtcgtggt gaagctgaaa ttgaaagtat tcgttctgct tcggaagatt
    24241 tccgccaacg gacaatggaa gtgctacggc tggcgttttt atcctccggc attctcgaat
    24301 tttttacctc gctgtcaatt gctctggtgg cggtctactt tggtttttcc tatctcggcg
    24361 agctggattt tggtcactac gataccggtg tgacgctggc tgcgggtttt ctggccctga
    24421 tccttgcgcc agagtttttc cagccattgc gcgatctcgg tacgttttat catgctaaag
    24481 cccaggctgt tggtgcagct gacagtctga aaacgtttat ggaaaccccg ctcgcccatc
    24541 cgcagcgtgg cgaggtggaa ttagcatcga ccgatccggt gaccattgag gccgaggatc
    24601 tgtttatcac gtcgccggaa ggtaaaacgc tggccggacc gctgaacttt actttgccag
    24661 caggccaacg tgcagtgttg gttggtcgca gcggttcagg taaaagctca ctgctgaacg
    24721 cgctttctgg ttttctctca tatcagggat cattacgaat caacgggata gaattacgcg
    24781 atttatcacc agaatcatgg cgtaaacatc tctcctgggt tgggcaaaac ccacaattac
    24841 cggcagcaac attgcgggat aacgtactac tggcgcgacc tgatgccagc gaacaagaat
    24901 tacaagcagc gctggataac gcctgggtca gcgagtttct accgctcctg ccacaaggcg
    24961 ttgatacacc tgttggcgac caggctgccc gcctttccgt ggggcaggcg cagcgcgtgg
    25021 cggtggcccg tgcgttacta aatccctgtt cgctattact gttggatgaa cccgctgcca
    25081 gccttgatgc tcacagtgaa cagcgcgtaa tggaggcgct gaatgccgcc tctctgcgcc
    25141 agacaacgtt aatggtcacc caccagttag aagatcttgc tgactgggat gtcatttggg
    25201 ttatgcagga tggccggatt attgagcaag gacgttacgc ggaattaagt gtggctggtg
    25261 gcccattcgc cacattactg gcccatcgtc aggaggagat ttaaatgcgc gctttgctac
    25321 cctatctggc actgtataaa cgtcataaat ggatgttaag tcttggtatc gtgctggcaa
    25381 ttgtcacgct gctcgccagt atcggtctgt tgacactttc cggctggttc ctctcggcct
    25441 cagcggttgc gggggttgcc ggactgtaca gcttcaacta tatgctaccc gctgcgggcg
    25501 tgcgtggcgc agcaatcacc cgtactgccg ggcgttattt tgaacgtctg gtaagtcacg
    25561 acgcgacttt ccgcgtgttg cagcatctgc gcatttacac cttcagcaaa ttgctgcccc
    25621 tctcccctgc cggactggcg cgctatcgtc agggcgaatt gctcaatcgc gtggtggcgg
    25681 atgttgatac gctcgatcat ctttatctgc gcgttatctc gccgctggtg ggcgcttttg
    25741 tggtgattat ggtggtgaca atcgggttaa gtttccttga tttcaccctc gcctttaggc
    25801 tgggcggcat tatgttactg acgcttttcc tgatgccacc gctgttttat cgtgcgggaa
    25861 aaagcaccgg gcaaaatctg actcatcttc gcggacagta tcgccaacaa ctgacggcct
    25921 ggctgcaagg gcaagctgag ctgaccattt ttggcgccag cgatcgttat cgcacgcaac
    25981 tagagaatac agaaattcaa tggctggaag cgcaacgccg tcaatctgaa ctgaccgcat
    26041 tgtcgcaagc gataatgctg ctcattggcg cgttagcggt gatcctgatg ctgtggatgg
    26101 cgtctggcgg cgttggcggc aatgctcaac ccggcgcgtt aattgccctg tttgtcttct
    26161 gcgcgttagc cgcgtttgaa gcactggcac cagtaacggg tgcatttcag catctggggc
    26221 aagtcattgc ctctgccgtt cggatcactg atttaacgga tcaaaaaccg gaagtcacct
    26281 tccctgatac ccaaactcgt gttgccgatc gcgtttcgct gacattacgg gatgttcagt
    26341 tcacttatcc ggagcaatcc caacaggcac ttaaagggat ttctcttcag gtaaacgccg
    26401 gggaacatat tgcgattctc gggcgaaccg gatgcggcaa atcaacactg ttacaactgc
    26461 tgacccgcgc ttgggacccg caacagggag agattttgct taacgatagc cccatagcca
    26521 gcctgaatga agcggctcta cgacagacca tcagcgttgt tcctcagcga gtgcatctgt
    26581 ttagcgccac gctgcgtgat aatctgttac tcgcctcgcc tggcagtagt gatgaggctc
    26641 tggcggagag cttgcgtcgc gttggtctgg agaagctgct cgaggatgca ggtctcaaca
    26701 gttggttagg tgaaggcgga cgccagctct ccggtggtga actgcgccgt ctggctatcg
    26761 cccgtgcgct gttacatgat gcgccactgg tgttgctgga tgaacctacc gaaggcttag
    26821 atgccacaac cgaaagccag atccttgaat tgcttgcaga aatgatgcgt gagaaaacgg
    26881 tgttaatggt cacccatcga cttcgcggac tctctcgttt ccaacaaata atagtgatgg
    26941 acaacgggca aattattgag caaggtactc acgcagagct gcttgccaga caggggcgtt
    27001 attaccagtt caagcagggt ttgtaagcta ttattgaacg atccgacttg cgtggagttt
    27061 tgcggtcatg cgcctggttc agctttctcg ccattcaata gccttccctt ccccggaagg
    27121 cgcattacgt gagcctaacg gcctgctggc acttgggggc gatcttagcc ctgcgcgcct
    27181 gttaatggct taccagcgtg gtatttttcc gtggttttct ccaggcgacc ccatcctctg
    27241 gtggtcgccc gatccccgcg cggtgctatg gccagaatca ctgcatatca gccgtagtat
    27301 gaagcgattt cataaacgct cgccctatcg tgtcacgatg aattacgctt ttggtcaggt
    27361 cattgaaggc tgtgccagcg atcgcgaaga aggaacctgg atcacgcgtg gcgtggtcga
    27421 agcctaccat cgccttcacg aactcggcca tgcccactcc attgaagtct ggcgtgaaga
    27481 tgagcttgtc ggcggtatgt acggcgtggc ccagggaacg ctattttgtg gcgagtccat
    27541 gttcagccgg atggaaaatg cgtctaaaac ggcgcttctg gtattctgtg aggaatttat
    27601 cggtcatggc ggtaagctta tcgactgcca ggtccttaac gatcacacag catcgcttgg
    27661 tgcctgcgaa attccccgcc gggattacct taattatctc aatcaaatgc gcctcggacg
    27721 attgccgaat aatttctggg taccacgatg cttgttttca ccacaagaat gaatgttttc
    27781 ggcacatttc tccccagagt gttataattg cggtcgcaga gttggttacg ctcattaccc
    27841 cgctgccgat aaggattttt tcgcgtcagg taacgcccat cgtttatctc accgctccct
    27901 tatacgttgc gcttttggtg cggcttagcc gtgtgttttc ggagtaatgt gccgaacctg
    27961 tttgttgcga tttagcgcgc aaatctttac ttatttacag aacttcggca ttatcttgcc
    28021 ggttcaaatt acggtagtga taccccagag gattagatgg ccaaagaaga caatattgaa
    28081 atgcaaggta ccgttcttga aacgttgcct aataccatgt tccgcgtaga gttagaaaac
    28141 ggtcacgtgg ttactgcaca catctccggt aaaatgcgca aaaactacat ccgcatcctg
    28201 acgggcgaca aagtgactgt tgaactgacc ccgtacgacc tgagcaaagg ccgcattgtc
    28261 ttccgtagtc gctgattgtt ttaccgcctg atgggcgaag agaaagaacg agtaaaaggt
    28321 cggtttaacc ggccttttta ttttgtgata tgtatgaagt actttggaag tataagtccg
    28381 taacttgtct cgatgtagat atactgcaca acttgtttac agtatgagca aacaataatt
    28441 atcattaaaa ttcttcttga ccgttttact ccaactccct atagtagcgc cccgttgcca
    28501 cccatgaggt ttggtagcaa aacaatttgg tgaggtgtcc gagtggctga aggagcacgc
    28561 ctggaaagtg tgtatacggc aacgtatcgg gggttcgaat ccccccctca ccgccatatt
    28621 taaagaagag ctcgtacgaa agtgcgagct tttttttcgc attttatctg tctgtacggc
    28681 agtgaacgcg ttcgcaaagg ttttaccgca ccccgtcgat ttctaagctg cctgtacggc
    28741 agtgaactca aatactacac gtttagcccg ttgttaataa agcaaaacca tcccattctc
    28801 ctgcagcacc cttttttatc cttaccttcc cgcagcacta attatcaaca acttaccatc
    28861 atcattaaaa aagggtctga acctcctcca ccccataaac aaaaagtccg taacctcttt
    28921 cgagattacg gacttgacca acctacctaa caatcagatt aatgcgctgc ttccgccttg
    28981 tgcttttgtg cactctggaa tccgtaagtc agctcatttt tctctttatc cagcgcgacg
    29041 gtgacctgac cgccgtccac cagcgaacca aacagcagtt cgttggcgag cggttttttc
    29101 aggttgtcct ggatgacacg cgccatcgga cgtgcgccca ttgcccggtc gtaacctttc
    29161 tcggccagcc agttacgcgc ttcctggctc acttccagag aaacaccttt ctgatccagc
    29221 tgaacctgca actcgacgat gaatttatcc accacctgat ggatcacgtc ggttgacaga
    29281 tgatcaaacc agataatgtt gtcgagacgg ttacggaatt ccggtgtaaa gatcttcttg
    29341 atctcctcca tcgcatcggt gctgttatcc tggtggataa gaccaatgga tttacgctca
    29401 gtttcacgta ccccggcgtt ggtggtcatc accagcacca cgttacggaa gtccgctttg
    29461 cgtccgttgt tatcggtcag cgtaccgttg tccatcacct gcaacagaat attgaacacg
    29521 tccggatgcg ctttctcgat ttcgtccagc aacagcaccg catgtggatg cttgatgacc
    29581 gcatcagtca gcaaacctcc ctgatcaaaa ccaacgtatc ccggaggcgc accaatcaga
    29641 cggctgacgg tatggcgttc catatactcg gacatatcaa agcgcagcag ctcaatgccc
    29701 aacgctttcg aaagctgtac cgtcacctct gttttcccga ccccggtagg gccggcaaac
    29761 aggaacgaac caaccggttt atgttcgtga cctaaacctg cacgcgccat cttaatggct
    29821 tcagtcagcg cctcaatggc tttatcctga ccgaagacca gcattttcag gcgatcgccg
    29881 aggtttttca gggtatcgcg gtcactctga gaaacactct tctctggaat gcgcgcaata
    29941 cgggccacca cggactcaat atccgccaca ttaacggttt tcttgcgttt gcttaccggc
    30001 atcaggcgtg cgcgagcgcc cgcttcgtcg ataacgtcaa tggctttatc cggcagatga
    30061 cgatcgttaa tgtatttcac cgccagctct accgctgcac gcaccgcttt tgcggtataa
    30121 cgcacgtcgt ggtgcgcttc atacttcggt ttcaggccat tgatgatttg aacagtttct
    30181 tcgatcgacg gttcagtaat atcaattttc tggaagcgac gcgccagagc acggtctttc
    30241 tcgaaaatgt tgctgaactc ctgataggtt gtcgaaccaa ttacgcgaat tttaccgctg
    30301 gagagcaacg gtttgatcag gttagccgca tcgacctggc caccagacgc tgcacccgca
    30361 ccgataatgg tgtggatctc atcaataaac aggatgctgt tagtgtcctg ctccagctgc
    30421 ttgagcaacg ctttaaaacg tttttcaaag tcgccgcgat atttagtgcc cgctaacaga
    30481 gaaccgatat cgagagagta aatcgtacag tcagccatca cttccggcac atcgccctga
    30541 acaattcgcc aggcaagacc ttccgcaatc gcggttttac cgacaccaga ttcccccacc
    30601 agcagcgggt tgtttttacg gcgacggcag agaacctgaa tagcacgctc cagctccttc
    30661 tcacgaccaa tcagtgggtc gattccgccc acgcgcgcaa gctgattcag gttcgtcgtg
    30721 aaattctcca tacgttcctc cccaccagct tgttcttcgc tgtttggctg gctgccagga
    30781 tcagaagact gtgtcggctc gtctttacgc gtgccatgag agataaagtt caccacatcg
    30841 agacggctga cttcatgttt acgcaacaga tatgccgcct gcgactcctg ttcgctaaag
    30901 atagcgacca gaacgtttgc accggttacc tcattgcgac cggaggactg gacatggaag
    30961 accgcacgtt gcagtacacg ctgaaaactc agcgtcggct gtgtgtcgcg ctcctcttca
    31021 ctggcaggca gaacgggtgt ggtttgttca ataaaggctt ccagttcctg acggagcgca
    31081 accaaatcca cagaacacgc ttccagcgcc tcccgggcag atgggttact gagcagcgcc
    31141 agtaacaagt gctcgacggt cataaactca tgacggtgct cgcgcgctct ggcgaaagcc
    31201 atatttaaac tgagttccag ttcttgattg agcataggca cctcccccaa tttttatgcc
    31261 tgcattcagg ctttttctag cgtacacagc aatggatgct cattctccct cgcgtacttg
    31321 ttcaccatcg ccactttggt ttctgcaacc tcggcggtaa agactccgca aatggccttc
    31381 ccctggtagt gaacagcgag catcaattgc gttgcacgtt ctacatcata agaaaagaat
    31441 ttttgtaaca cgtcaataac aaactccatc ggagtgtaat catcattgac taatatcact
    31501 ttatacatag atggcggttt tagcgcgtcg cgaacttttt cttccgccag ttgatcaaag
    31561 tccagccagt cgttcgtttt acccattgtc agttatcatc ttcggttacg gttatcggca
    31621 gaacgccctg ccgctgacaa gagcttattt gcctgaccaa tctactccac atgatagata
    31681 actatcatct atcgttggca tcagcgacat ctgtcacatt cctgtcaata gcgttaactg
    31741 cttcaaattt ttgattcata cctgccccct ggcccctgtc aaacgcttga cggctcgccc
    31801 taattctcta aattgtattt ctagagttgg cgaggttttg aacagccccc tctctgaccc
    31861 cggtttattc catcttactt gtataagatt tgcgaaggat gtcgaagcat ggaaaagggt
    31921 actgttaagt ggttcaacaa tgccaaaggg tttggtttca tctgccctga aggcggcggc
    31981 gaagatattt tcgctcatta ttccaccatt cagatggatg gttacagaac gctaaaagct
    32041 ggacaatccg ttcagtttga tgtccaccag gggccaaaag gcaatcacgc cagtgttatt
    32101 gtgcccgtcg aagtagaagc ggcagtcgca tagctcttct gtctcattgt gtacatccta
    32161 aaggcaaaat gccagcccga tcggctggca tttttatctc aaaaattact ctcgtgccag
    32221 agcatctact ggatccagtc gtgccgcatt tcgtgcgggt aaccatccaa ataaaatccc
    32281 ggtgaccgtc gagcagagaa acgccagcag cagtgccaac ggtgaaaaac caatctccca
    32341 gccgggtaag aaaagctgca aggtgaaagc aattaacagt gacagtgtta ttcccaacgc
    32401 gccaccgacc aggcaaacca gtacggcttc gatcaggaac tgttgcaaaa catcgcttgc
    32461 tcgcgcacct acagccatgc gaatgccaat ttcccgcgtc cgctcggtca ctgacaccag
    32521 cataatattc attacaccaa taccgcccac caccagcgaa atcaccgcca ccagcgtcag
    32581 aaacagttgt aaagtacgtg tggtcttttc aacagttttc aagacgccgt ccatgttcca
    32641 ggtaaagaaa tccttctttc cgtggcgcag tgaaagtaaa cgcgtgagtt gctgttccgc
    32701 ctcggcgctg tcaaatcctt cttttaccct gacagtaatg gagttcagcc acgactggcc
    32761 cataactcgc ccggacatcg tgctgtaagg tagccagaca cgcagcactt tactgctacc
    32821 aaacatcgac tgtttttctt ccgccacacc aatgactctg gcgggcatat tgccgaccag
    32881 aatcacctcg ccaaccacat ctgctttatg ggggaaaagc tggcggcgag tattactgtc
    32941 gagaaccacg acctgcgcac gaccgttcag ctgctcctga ttaaaggtgt ttccttcact
    33001 gaaggtcatg ccatagacat taaaataatc gccgctcacg ccattggcac tggcagcaac
    33061 atcaacattg ttataacgca ggcgcaggtt ttgcgagacg gcaggtgtgg ctgaggcgac
    33121 ccacggttgt ttttggatgg cgattaagtc gtcgtacttc agcgcctgct gatattgcgg
    33181 atcgtcatcg ccaaaatctt tcccgggata gacatcaata gtattcgtac caatagaacg
    33241 aatatccgcc agcaccattt gtttggcggc gtcacccacc acgacaatgg aaaccaccga
    33301 cgcaataccg ataataatcc ccagcatggt cagtaaagta cgcattttat tcgctgccag
    33361 cgcccgccat gccatcgtca gcgcctcgtt aaaaccgctg acaaactgcc gccagccaga
    33421 caccgtgttg acaacaggct ccgtcccgcc agcaacattc actttttcaa tggcgggagg
    33481 attgcgcaca atttcgccgt cgcgaatttc gatcacccgc tcggcctgag cagcgacctg
    33541 cggatcgtgg gtgacgataa tcaccgtatg cccacgatcg cgcagctgat gcaggatcgc
    33601 catcacctct tcgccagaat ggctgtccag tgcgccggtt ggttcatcgg caagaattac
    33661 ctgaccaccg ttcatcaatg cccgcgcgat gctgacgcgc tgttgctgac cacccgaaag
    33721 ctgtgccgga taatactctg tacggtcttc cagccccagc cgttgcagca actcctgggc
    33781 acgaagcagt cgctgtttcc gctcaagacc agcatagacg gcgggtactt caacgttctg
    33841 ctcggcggtt aaatgcgaaa gtaaatggta acgctggaaa ataaagccga aatgctcgcg
    33901 gcgcagttgc gccagcgcat cggcgtccag tgtggcaaca tcctgaccgg cgacgcgata
    33961 ggtgccgctg gtggccttat ccagacagcc gagaatattc atcagggtcg atttaccgga
    34021 acctgaagcg ccaacaatcg ctaccatctc accggcataa atatcgaggg tgatgccctt
    34081 cagcacctca acctgctcat caccggcagg atagctgcga cgaatatcct ttaattcgag
    34141 caaaggcgtc attgtgcagc tcctggtttg gcctcaccaa tcaccacttc atcgcccgct
    34201 tcaagccctt tgacaatctc aacatcggta tcgttacgtg cgccaatcgt cacttcacgc
    34261 tcgcgtgttt caccattacg caacaatttg actttataac gattatcgcc aaccggatcg
    34321 cctaacgccg acagagggat cgtcagcaca tttttcacat cggtgagctg aatatgcact
    34381 tgcgcagtca tatccagccg cagcaaacca ttggggttgg ggacttcaaa acgggcgtaa
    34441 tagaaaatag cgtcgttaac cttttccggc gtcggtagta catccttgat ttgcccctcg
    34501 tagcgcgtca gtggatcgcc aagtaccgta aaccaggctt tttgccccgg cttcaggtgg
    34561 attacatccg cttcagaaac ctgcgctttt accagcatgg tgctcatatc tgccagcgtc
    34621 agaatgttcg gtgcttgttg tgcggcaatc accgtctggc cttgcagagt ggtgatttgc
    34681 gtgacttccc cggccatcgg ggcaacgatg cgagtgtaat cgagattggt tttagccgta
    34741 tcgagagagg cctgattgcg cttgatttgc gcgtcaatgg tgccaatttg cgcctgtttc
    34801 acagccatct ccgtcgcggc ggtgtcgaga tcctgctgtg aaaccgcctg cgtttgtgcc
    34861 agacgttgct gacgggaata cgtcacccgc gccagtttca gctccgcttc cgcctgctgc
    34921 cgctgcgcac gtagctccat cagcgttgct tcgacctcct taatctggtt ttcagcctgt
    34981 tcaggatcaa taacccctaa aagctggtct ttttttactt tatcgccaat cgccaccgac
    35041 agagttttca actgaccgct gacctgcgcg cccacgtcaa ccttacgcag cgcgtccagc
    35101 tttccggtcg ccagcacgct ttgctgtaaa tcaccggggc gcacaatcag tgtctgataa
    35161 gtcggcacgg gtgcgttaag aattctccat aacgtaatca gtccggcgat gactatcacc
    35221 agcgcaataa cgtaacgctt cttcacggtt ttccgctttt tcataaatat tctgaatact
    35281 ccatatgcca atccgggtca ttaacttcaa cgaaatatca acaaaaccct aaaatttacg
    35341 ttagtaatca cactattgat gtttggttaa gatacgcaat ggtgaaatcg acgtcatgta
    35401 taaccattga tttcatgaat atgtcgcagc taactgaacg gacctttacg ccatctgaat
    35461 ctctcagcag cctgtcactt tttcttagtc tggcacgtgg acagtgtcgg ccgggtaaat
    35521 tctggcatcg ccgtagtttt cgccagaaat ttttgctgcg ctcgttgatt atgccgcgtt
    35581 taagcgttga gtggatgaac gaactttccc actggcctaa tctcaatgtg ttgttaacgc
    35641 gccaaccgcg actgcctgtg cgtctgcatc gcccttacct tgcggcgaat cttagccgta
    35701 agcaattgct ggaggcgtta cgttaccatt atgcgttact ccgcggatgt atgtcggcgg
    35761 aagaattcag cttatatttg aatacccccg ggctgcaact ggctaaactg gaaggcaaaa
    35821 acggcgagca gttcacgctt gagctgacca tgatgatctc aatggataaa gaaggtgaca
    35881 gcacaatcct gttccgcaac agcgaaggta ttcctctggc agagctcact tttaccctgt
    35941 gtgaatatca ggggaaaaga acgatgttta ttggcggact gcaaggcgca aaatgggaaa
    36001 tcccacatca ggaaatccag aatgcgacga aagcctgcca cgggctattt cccaaacgcc
    36061 tcgtgatgga agcggcctgt ctgtttgccc aacgtttgca ggtagagcag attattgccg
    36121 tcagcaatga aacgcatatt taccgcagcc tgcgttatcg tgataaagaa ggcaagatcc
    36181 atgccgatta caacgctttc tgggaatcgg ttggcggcgt atgtgatgct gaacgccatt
    36241 accgccttcc agcacagata gcacgaaaag agattgccga aatcgccagt aaaaaacggg
    36301 ctgaataccg tcggcgctat gagatgctcg acgctattca gccacaaatg gccacgatgt
    36361 ttcgcggtta atccgcgcga ccgcgcgcca gccacagcac gcgtgagaac atttttttca
    36421 gcagcgtcgg tacggagtcc acaccacgac gtccggcctc cattgccact tcaatggcaa
    36481 gatcgggttt ggaagagcta tggatcgctt tcgagataat tttgcgcaga ttcatcggta
    36541 cattttccgg gatttgcgcc acgcggtgga acacatcgga aaatccctgg cgatacataa
    36601 aatgttccat atccagcgcc ggtaacgccg ttaaatgttc tcgttcggct tcccgatcgt
    36661 tattcaacag gctgcgtacc gtagcggcat atttcttccc tgcttcatcg ccatcgacca
    36721 gtacatgcca ttcaatcccc attcggcggg caaatttaac cagtggcttt agcccggact
    36781 gggcaaactc aatgaccttg atcccttcgg catcgaaatg gtgtccacac tggcgcgcca
    36841 gttcattgat aacccaggtt tccgtttccc cttccaccag caaccagcag cgagcaaaca
    36901 gcgatgacgg acggttaaaa cgaatgtgaa aggatatgcg tcggctatct tcggtactca
    36961 agccactcgg ccccagacgc caggcggcaa cgcgcgagga ctcacgtacc agtcggcaaa
    37021 catgctctac cggcgttaac gaaagcaact cacccgagtt ggtggtggca atgcgctgca
    37081 atggcagaag attcaacaga tgccaggcaa ctgaaagcat aatggggtgt aaacgggttt
    37141 ctggatcttc aattagcaat aacggacgcg cgtctttatc cagtctcaat gtgccttttg
    37201 cctgcaataa agtagcaaac aggccgagca aaataacccg atacgagcgc cctccaggtc
    37261 ggtcaatcat ccggttgatg atgtccagat agcgccagct tcgttgctca ttgctggccc
    37321 gccgccgcat taaacgatat cgcgcctgtc cagccccctg ctcagagaaa taatgctcaa
    37381 gcaactgcac cattgcggaa agtccctgac gaatctgtcc atcagagaga ttttgcggat
    37441 gtgaggacaa ctcacgggcg aggaaatcga gctggcgcgc ggtgacttcc acattgggaa
    37501 catttggcac cgtgccgtta cgaatacggc gcataaaacg ggcatcacgc aagcgcagca
    37561 ccggcattaa acgcaccaga tggcgtgcct gatcgttaat atcctcgaca tcaatcggat
    37621 gtccgtcttt atcgagaaaa ctgcgcagtg tcatcacgct gccgtcttcc gcactctccc
    37681 cttccagacg ataaaaaata cggtgatagc catcggtgca tggcgtccag cacgcttcca
    37741 gcggccgata acggcgaacc cgatgtcggc ctggcagcga ttcgcggaag gtcaaaataa
    37801 tatgcagatg atgttctcgc ccgttgatat ctcccggcgg gaaccagaaa tcgtcgcgct
    37861 caaaatggta gagatctgat tctggcgata gcagcagggt taaggcgtcc agcaagctgg
    37921 atttacccca cgcgttctcc ccaatcagga cgttgttttg ttcgagcatc aacgacaaac
    37981 ggttgatacc gcgaaaaccc acaatttcaa cgcgctcaag aatcatatcc cctccagaaa
    38041 tatgtgactt atcctttacg ctggcagtat aacgggaaag gccttgctaa tacgtgatga
    38101 catcacattt aagcatggca aatgtctgaa aaatgagcga ataatcgcgt tatactccct
    38161 cttgctgata agtctgcaaa aacaacaaaa tcaaataatt agcatgataa attatttttt
    38221 attacgccta aaggattaaa tcttttgctt gcactacact gtcaatcaga caaatgacta
    38281 tatggggcaa cccgctgttg ccctaaatca aatttactgg atttatttat ctggtgaatg
    38341 actcgccttc gtttttaaaa tagccgcgca ttatttccgt cgtcactttt ttacgacgac
    38401 cctataaaac gaccatattt ttcacagggt caatttttaa ttgaggtgga tatgttcaga
    38461 aaattagcag ctgaatgttt tggtactttc tggcttgttt ttggtggctg tggtagtgct
    38521 gtactggccg caggcttccc ggaattaggc attggttttg ccggcgtggc gttggcgttc
    38581 ggtctgaccg ttctgacgat ggcctttgct gttggtcata tttctggtgg tcattttaac
    38641 ccggcggtca ctattggttt atgggctggc ggacgttttc cggcaaaaga agtcgttggc
    38701 tacgtaattg cccaggttgt cggcggtatt gttgcagcgg cgctgctgta tttaattgcc
    38761 agtggtaaaa cgggttttga cgcggcagcc agcggttttg cttctaacgg ttatggcgag
    38821 cattcaccag gcggttattc catgctttcc gcgctggtag ttgaactggt attgagtgca
    38881 ggtttcctgt tggtgatcca cggcgcaacc gacaaattcg cgccggcagg ttttgcgccg
    38941 atcgctattg gtctggcctt aaccctgatt cacttaatta gtattccggt gactaacact
    39001 tctgttaacc cggcgcgcag caccgcggtt gctatcttcc agggcggctg ggcattagaa
    39061 caactgtggt tcttctgggt ggtgccaatt gtcggcggca ttatcggtgg tctgatttac
    39121 cggaccctgc tggaaaagcg tgattaatta acgcagaagg cctggcgtaa tgtcaggcct
    39181 ttttttaaga tgttgttata tgttgattca tcatcttgtt gcgctccttt catgagctaa
    39241 gccacatatt gccactggcg caaggagcgc gcagggggcg gccaatcgcc gccgccccat
    39301 gcacccccgg gctctggcga acaaaatcgc cgctgcgcgg tgccctcgac ttatccctta
    39361 cggctaccgg gtcgggcgcg aggtaacatc cctgtaaaac gcgccctcag cccacatcca
    39421 tgtgggctgc cccggccttc agggaacgcc tcggcgattt tgacgccacc aacatcggtg
    39481 cagtttgtaa ttaaagcgca agaatatctt taaatgaaaa cgaatactct actgctcctt
    39541 atgcataaca tttcctttta attgcaactt tactcgtcct tccgctttca gtagtgtgtc
    39601 atcgcgcatt tcgacttcat ctttgcaagg actgggtttt catgttttct gggctgttaa
    39661 tcattctggt tcccctgatt gtgggttacc tcattccgct tcgccaacaa gctgcgttaa
    39721 aagttattaa tcagctatta agctggatgg tttaccttat tctctttttt atgggtataa
    39781 gtctggcgtt cctcgataac ctcgccagta acctgttggc gattctgcat tattctgccg
    39841 tcagtattac cgttatttta ctgtgtaata ttgccgccct gatgtggctg gagcgaggcc
    39901 tgccgtggcg caaccaccat cagcaagaaa aactcccgtc gcgtattgcg atggcgctgg
    39961 agtcgctaaa actgtgcggc gtagtagtga ttggttttgc cattggtctg agtggactgg
    40021 ctttcttaca acacgcgacc gaagccagtg aatacacgtt aattttgcta cttttcctcg
    40081 ttggtattca gttgcgcaat aatggcatga ccttaaagca gattgtcctt aatcgccggg
    40141 gaatgattgt cgccgtggtg gtggttgtca gttcattaat tggtggttta attaacgcct
    40201 ttattcttga tctccccatc aatacctcgc tggcaatggc ctccggtttc ggctggtatt
    40261 ctctttccgg tattttattg accgaatctt ttggtccggt aatcgggagc gcggcgtttt
    40321 ttaatgatct ggcccgtgaa ctgattgcta ttatgttgat ccctgggctg attcgccgca
    40381 gccgctctac tgcactgggc ttatgcggtg ccacatcaat ggatttcacc ctgcccgttc
    40441 ttcaacgtac tggcgggctg gatatggtcc cggcggcaat tgttcacggt tttattctta
    40501 gcctgttagt gccgatcctc atcgcctttt tctctgcgta atacctctct ggcggtagat
    40561 tcctgccgcc aaaattgcgc taaatcaatc tcccttaaag ttgcatgaaa aatccctttt
    40621 atccccgcgt taagcgtctt aaccttaaac atgtatatta aatataactt taaaaggtgt
    40681 gatcatgttt tgtgtgcaat gtgaacaaac tatccgtact ccggcaggaa acggctgctc
    40741 atacgcgcag gggatgtgtg gtaaaacggc ggaaacttct gaccttcagg atttactcat
    40801 cgcggcgctg caagggcttt ctgcctgggc ggtaaaagcg cgtgaatacg gcatcatcaa
    40861 ccacgatgtt gacagcttcg cgccacgtgc ctttttctca actctgacca acgttaactt
    40921 cgattctccg cgtattgtcg gctacgctcg tgaagcgatt gccctgcgcg aggcgctgaa
    40981 agcgcaatgc ctggctgtag atgccaacgc gcgcgtcgat aacccgatgg ctgacctgca
    41041 actggtgagc gacgatctcg gcgagctgca acgtcaggct gcagaattta cccctaacaa
    41101 agataaagcg gcgattggcg aaaacattct cggcctgcgt ctgctgtgcc tgtatggcct
    41161 gaaaggtgcg gcggcctata tggaacacgc gcatgttctc ggtcaatacg acaacgatat
    41221 ttatgcccag tatcataaaa tcatggcgtg gctggggacc tggcctgccg atatgaacgc
    41281 gcttcttgag tgttcaatgg aaatcggcca gatgaacttc aaagtgatga gcattctgga
    41341 tgcaggcgaa accggtaaat acggtcaccc gacgccaacc caggtcaacg tcaaagcgac
    41401 ggcgggtaaa tgcattctga tttccggtca cgatctcaaa gatctctaca acctgctgga
    41461 acagaccgaa ggcacgggcg ttaatgtcta cacccacggc gaaatgctgc ctgcgcatgg
    41521 ctacccggag ctgcgtaaat tcaagcatct ggtcggtaac tacggcagtg gctggcagaa
    41581 tcagcaagtg gagttcgctc gtttccctgg ccccatcgtg atgacctcga actgcatcat
    41641 cgacccaacc gtaggcgctt atgacgatcg tatctggacc cgcagcattg ttggctggcc
    41701 tggcgtgcgt catctggacg gtgatgattt ctctgcggtt atcacccagg cgcaacagat
    41761 ggcaggcttc ccgtacagcg aaattccgca ccttatcacc gtgggttttg gtcgccagac
    41821 gctgcttggc gctgctgata cgctgattga tctggtgagc cgtgaaaaac tgcgtcatat
    41881 cttcctgctt ggtggctgtg acggcgcacg cggcgagcgc cactacttca ccgatttcgc
    41941 caccagcgtg ccggatgact gcctgatcct gaccctcgcc tgtggtaaat atcgctttaa
    42001 caaacttgag tttggcgata tcgaaggtct gccgcgtctg gtagatgcag gtcaatgtaa
    42061 cgatgcttac tcagcgatta ttctggctgt cactctggca gagaaactgg gctgcggtgt
    42121 caacgatctg ccgctgtcgc tggtgctctc ctggtttgaa cagaaagcga tcgtcattct
    42181 gctgacgctg ctttctctgg gcgtgaaaaa tatcgtcacc ggcccgactg cacctggttt
    42241 cctgacacca gacctgctgg cggtgctgaa cgagaaattt ggcctgcgtt ctatcaccac
    42301 tgttgaagaa gacatgaagc aactgttgag cgcgtaagga gtttgtcgat gacgatgcca
    42361 acgaatcaat gcccgtggcg gatgcaggtt catcacatta cgcaagaaac gccggatgtg
    42421 tggacgattt ccctgatttg ccacgattac tacccatatc gcgccgggca atatgcactg
    42481 gtcagcgtgc gaaactcagc ggaaacgctg cgtgcttaca ccatttcctc cacgccaggc
    42541 gtgagtgaat atatcaccct gaccgtgcgg cggattgatg acggtgtcgg ctcccagtgg
    42601 ctgacgcgcg atgtaaaacg cggtgattat ctctggcttt cggacgcgat gggggaattt
    42661 acctgcgacg ataaagcaga agataaattc ctgttgctgg cggcaggctg cggcgtcacg
    42721 ccgattatgt cgatgcgtcg ctggcttgcg aagaaccgtc cacaggccga tgtgcgggtg
    42781 atctacaacg tgcgtacgcc gcaggatgtt attttcgccg atgagtggcg taactatccg
    42841 gtaacgctgg tggcagaaaa taacgttacc gaaggcttta tcgctggtcg tctcactcgc
    42901 gaactgctgg caggtgtacc tgacttagct tcacgtaccg tgatgacctg cggcccggct
    42961 ccgtatatgg attgggtaga gcaggaagtg aaagcgctcg gcgtgacgcg tttctttaaa
    43021 gagaaattct tcaccccagt agcagaagcg gcgaccagcg gtctgaaatt caccaaactg
    43081 caaccggcac gagaatttta cgccccggtt ggcaccacgc tactggaggc gctggaaagc
    43141 aataacgttc cggttgtcgc tgcctgccgt gcgggtgttt gcggctgctg taagacaaaa
    43201 gtggtttccg gtgaatatac ggtgagcagt acaatgacgc tgaccgacgc cgaaatcgct
    43261 gaaggttacg tactggcctg ctcctgccat ccgcaggggg atttggttct cgcataatcg
    43321 ccttatgccc gatgatattc ctttcatcgg gctatttaac cgttagtgcc tcctttctct
    43381 cccatccctt ccccctccgt cagatgaact aaacttgtta ccgttatcac attcaggaga
    43441 tggagaacca tgaaacaaac ggttgcagct tatatcgcca aaacactcga atcggcaggg
    43501 gtgaaacgca tctggggagt cacaggcgac tctctgaacg gtcttagtga cagtcttaat
    43561 cgcatgggca ccatcgagtg gatgtccacc cgccacgaag aagtggcggc ctttgccgct
    43621 ggcgctgaag cacaacttag cggagaactg gcggtctgcg ccggatcgtg cggccccggc
    43681 aacctgcact taatcaacgg cctgttcgat tgccaccgca atcacgttcc ggtactggcg
    43741 attgccgctc atattccctc cagcgaaatt ggcagcggct atttccagga aacccaccca
    43801 caagagctat tccgcgaatg tagtcactat tgcgagctgg tttccagccc ggagcagatc
    43861 ccacaagtac tggcgattgc catgcgcaaa gcggtgctta accgtggcgt ttcggttgtc
    43921 gtgttaccag gcgacgtggc gttaaaacct gcgccagaag gggcaaccac gcactggtat
    43981 catgcgccac aaccagtcgt gacgccggaa gaagaagagt tacgcaaact ggcgcaactg
    44041 ctgcgttatt ccagcaatat cgccctgatg tgtggcagcg gctgcgcggg ggcgcataaa
    44101 gagttagttg agtttgccgg gaaaattaaa gcgcctattg ttcatgccct gcgcggtaaa
    44161 gaacatgtcg aatacgataa tccgtatgat gttggaatga ccgggttaat cggcttctcg
    44221 tcaggtttcc ataccatgat gaacgccgac acgttagtgc tactcggcac gcaatttccc
    44281 taccgcgcct tctacccgac cgatgccaaa atcattcaga ttgatatcaa cccagccagc
    44341 atcggcgctc acagcaaggt ggatatggca ctggtcggcg atatcaagtc gactctgcgt
    44401 gcattgcttc cattggtgga agaaaaagcc gatcgcaagt ttctggataa agcgctggaa
    44461 gattaccgcg acgcccgcaa agggctggac gatttagcta aaccgagcga gaaagccatt
    44521 cacccgcaat atctggcgca gcaaattagt cattttgccg ccgatgacgc tattttcacc
    44581 tgtgacgttg gtacgccaac ggtgtgggcg gcacgttatc taaaaatgaa cggcaagcgt
    44641 cgcctgttag gttcgtttaa ccacggttcg atggctaacg ccatgccgca ggcgctgggt
    44701 gcgcaggcga cagagccaga acgtcaggtg gtcgccatgt gcggcgatgg cggttttagc
    44761 atgttgatgg gcgatttcct ctcagtagtg cagatgaaac tgccagtgaa aattgtcgtc
    44821 tttaacaaca gcgtgctggg ctttgtggcg atggagatga aagctggtgg ctatttgact
    44881 gacggcaccg aactacacga cacaaacttt gcccgcattg ccgaagcgtg cggcattacg
    44941 ggtatccgtg tagaaaaagc gtctgaagtt gatgaagccc tgcaacgcgc cttctctatc
    45001 gacggtccgg tgttggtgga tgtggtggtc gccaaagaag agttagccat tccaccgcag
    45061 atcaaactcg aacaggccaa aggttttagc ctgtatatgt tgcgcgcaat tatcagcgga
    45121 cgcggcgatg aagtgatcga actggcgaaa acgaactggc taaggtaaaa agggtggcat
    45181 ttcccgtcat aataaggaca tgccatgatt gatttacgca gtgataccgt tacccgaccg
    45241 agccgcgcca tgctcgaagc gatgatggcc gccccggttg gggacgacgt ttacggagac
    45301 gaccctaccg ttaatgctct gcaggactat gcagcagagc tttccggtaa agaagccgcc
    45361 atttttctgc ctaccggcac tcaggccaac ctggtcgctc tgctcagtca ctgcgaacgt
    45421 ggcgaagagt atattgtcgg tcaggccgcg cataactatc tgtttgaagc cggtggcgca
    45481 gcggtgctgg gcagtattca gccgcaaccc atcgacgcgg ctgccgacgg cacgctaccg
    45541 ctggataaag tggcgatgaa aatcaaaccc gacgatatcc atttcgcccg caccaaatta
    45601 ctcagtctgg aaaacaccca caacggcaaa gtgctgccgc gtgaatacct gaaagaagca
    45661 tgggaattta cccgcgagcg caatctggcg ctgcatgttg acggtgcgcg catctttaat
    45721 gccgtggtgg cttacggctg cgaactgaaa gagatcacgc aatattgtga ttcgttcacc
    45781 atttgcctgt cgaaaggtct tgggacgcca gtcggttcat tactcgtcgg taatcgtgat
    45841 tacattaaac gtgccatccg ctggcggaaa atggccggtg gcgggatgcg tcagtccggg
    45901 attctggctg ccgccgggat gtatgccctg aaaaataacg tcgcacggtt gcaggaagat
    45961 cacgacaacg ccgcctggat ggcggagcaa ctgcgtgaag caggcgcgga tgtgatgcgt
    46021 caggacacta atatgctgtt tgttcgcgtc ggggaagaaa atgctgccgc gttaggcgaa
    46081 tacatgaaag cgagaaacgt gctgattaac gcctcgccga ttgtccgcct ggtgacgcat
    46141 cttgacgtct cgcgcgaaca actggcggaa gtcgccgccc actggcgcgc attcctggcg
    46201 cgttaaggag agagacgtgc cgcaacgcat tttagttctc ggtgccagtg gctacattgg
    46261 tcagcatctg gtgcgcacac tcagccagca agggcatcag atcctggcgg cggcacgtca
    46321 tgtcgacagg cttgcaaagc tgcaactggc aaacgtcagt tgccataaag tcgatctcag
    46381 ctggccggat aaccttccgg ccctgttgca ggatatcgat accgtctatt ttctggtgca
    46441 cagcatgggc gaaggcggcg attttatcgc tcaggagcgc caggtggctc tcaacgtccg
    46501 cgatgcgcta cgtgaagtac cagttaagca attaatcttt ctcagttcgt tgcaggcccc
    46561 gccacatgag cagtcggatc atctgcgtgc tcgtcaggct acggcggaca ttcttcgtga
    46621 agcgaatgta cctgtgaccg aacttcgggc cggaattatc gttggcgcag gttcagcggc
    46681 gttcgaagtc atgcgcgata tggtctacaa cctgccagtg ttaacgccgc cacgctgggt
    46741 acgttcacgc accacgccca tcgcgctgga aaacttgctg cactatctgg tggcgttgtt
    46801 agatcatccg gccagcgaac accgcatctt cgaagccgcc ggaccagagg tgctcagtta
    46861 tcagcaacag tttgaacatt ttatggcggt gagcggtaag cgccgctggt tgatccccat
    46921 ccccctcccc acccgctgga tttcggtgtg gtttctcaat gtgattactt ccgtaccgcc
    46981 caccaccgcc agggcgttga ttcaggggct gaaacacgat ctgctggcgg atgataccgc
    47041 gctacgtgca ctcatcccac aacggctgat cgctttcgat gacgcggtac gtagcacgtt
    47101 gaaagaggag gaaaaactgg tcaactccag cgactggggc tacgacgctc aggcctttgc
    47161 ccgctggcga ccagagtacg gttattttgc caaacaggcg gggtttaccg ttaaaacgtc
    47221 cgccagcctt gctgctttat ggcaggtagt gaaccaaatc ggcggtaaag agcgttattt
    47281 ctttggcaat attttgtggc agacacgggc gttgatggac cgcgcgatcg gtcataagct
    47341 ggcgaaaggc cgcccggagc gcgaatattt acagactggc gatgcggtgg atagctggaa
    47401 agtgattgtc gttgaaccgg aaaaacaact tacgttgtta tttggcatga aagcgccggg
    47461 gctgggacga ctgtgtttta gcctggaaga taaaggcgac tatagtacta tcgatgtccg
    47521 cgctttctgg catccgcacg gtatgccggg gctgttttac tggttattga tgatccccgc
    47581 gcatctgttt atttttcgcg gaatggcaaa acaaatcgcc agactggcag aacaaagcac
    47641 agattaatcg acaaaacctg cgttattctt tcatgtttcc cattgcatca tgggggaaat
    47701 cacggaagaa tgcgcacgtt attttctcaa caacagtgga ttgatatgaa ggtactggtt
    47761 accggcgcca ccagcggctt aggtcgaaac gcggtagagt ttttatgcca gaaaggcatc
    47821 agcgtgcgag cgaccggtcg caacgaggca atgggcaaat tgctggaaaa aatgggcgca
    47881 gagtttgttc cggcggatct gaccgagctg gtttcatcac aagctaaagt gatgctcgcg
    47941 ggcattgata cgctgtggca ctgctccagc tttacctcac cctgggggac acaacaggct
    48001 ttcgatctgg ctaacgttcg cgccactcgc cgcctgggtg aatggactgt cgcctggggt
    48061 gtgcgtaact ttattcatat ctcttccccc tccctgtact tcgattatca ccaccatcgc
    48121 gatattaaag aagattttcg ccctcaccgc ttcgccaacg agtttgcccg cagcaaagca
    48181 gccagcgaag aagtgatcaa tatgctttcg caggcgaatc cacaaacgcg ctttactatt
    48241 ctgcgcccac aaagtctgtt cggaccgcac gataaagtct ttattccccg tctggcgcat
    48301 atgatgcacc actacggcag tattctgtta ccgcatggcg gcagtgcgct ggtcgatatg
    48361 acctactatg aaaatgccgt gcacgcaatg tggctggcaa gccaggaagc ctgcgataag
    48421 ctaccttccg ggcgtgtgta caacatcacc aacggcgagc atcgcacact gcgcagcatc
    48481 gtgcagaagc tgatcgacga gttgaatatt gactgtcgta ttcgttccgt cccctacccg
    48541 atgctggata tgatcgcccg cagcatggag cgtttaggcc gcaagtcagc aaaagagccg
    48601 ccgctgaccc actacggcgt ctccaagttg aattttgact ttacgctgga tattacgcgt
    48661 gcgcaggaag agttaggtta tcagccggtc atcaccctgg atgaaggtat cgagaaaact
    48721 gccgcctggc tacgcgacca cggaaaactg ccgcgctaat cctgcccgta tttctccagc
    48781 aatgcttcgg caatcgcctg agtttctgca tccgcttcgc cgttatataa cgtcgggcgg
    48841 aaatgcatct ggaatgccat aatcacgcgc cgctgctcgc gcggtgtcat atcaggttta
    48901 acgtcataac cgtagcgcgc caaaagctcc agcaatgacg cggtatctac cggtgtgtgc
    48961 ggcgcgcgcc cggcaaggta aaagttaacc cgctgcgcat ccggccaggc accaatcccc
    49021 tgctgcgcca gttgctgcca gggaaataat ggccccggat cgtctttgcg ctgcggtgcg
    49081 atatccgcat gtgccactac gttttccggc ttgatgtgat aacgggcaat aatatctttc
    49141 gccagtggaa taagcgcctg aatctgtgcc ggttcaaacg gggcaaaata tttcactccg
    49201 gccgattttt gccagccacg gttttccagc tcaatgccaa tagaggtgtc gttaaggcgc
    49261 gttgccccgc gccaggcgct aatccccgca tgccaggcca gttcttgttc cggcaccagt
    49321 tgccagatgc gcggtttacc gttgtatcgc ggtggtaccg cagggaccag ataatgcgag
    49381 ctgacctgct tatcggtcag tgtcgccagc gagctatcaa aatcatctgc ggtgtagtgg
    49441 atcaccagca ctttaatgcg cggatacgcc gcctgcgcct ggcgtcgggt atcaagctga
    49501 tatccctctt tctcgacaat gcctttttcg cctgcacacc ctgccagtaa cagagcggca
    49561 gcgatcagcc agaagactct tctcatcgac gcgttttcac cgccgtaccg ctgacgctaa
    49621 ccatcagcat actgccgttt tgcccgaccg tttcgtagtc gatatcaata ccgacgacgg
    49681 catcggcccc cagcgcccgc gcctgggagc ctaattcctc aaaggcgatc tcccgtgctt
    49741 tacgcagttc tttttcatac gcaccggaac gtccgccaac gatatcgcgg ataccggcaa
    49801 agaaatcacg gaaaatattg gcacctaaaa tcgcttcgcc ggtcaccaca ccgcaatatt
    49861 caacgatgat ctggccttcc agagttgggg ttgtagaaaa ttgcatgatc gatctccttg
    49921 tttgttaagc cttcgatctc taaagcatta tcagactgat acgctattat tgaaaggata
    49981 tcattattat cgtagaacgc tttcagagcg atcgcttata aggaaatcat tatgcgctac
    50041 agcaaattga caatgcttat cccctgcgca ctgctcctca gtgcctgcac cacagtcact
    50101 ccagcttata aagataacgg cacacgcagt ggtccttgcg ttgaaggagg cccggataac
    50161 gtggcccagc aattttatga ctaccgcatt ttgcaccgca gcaacgacat caccgccttg
    50221 cgcccttatc tgagcgacaa actggcgaca ctgcttagcg atgcctcccg ggataataac
    50281 catcgggaac tactgaccaa cgatccattc tccagtcgca caaccttgcc agagagcgcc
    50341 cgtgttgcca gtgcctcaac aatcccgaat cgtgatgcgc gaaatattcc tctgcgtgtg
    50401 gatctaaaac agggtgatca gagctggcag gatgaagtgt taatgattca ggaaggccag
    50461 tgttgggtga tcgacgatgt acgctattta ggcggcagcg ttcatgccac ggcgggtacg
    50521 ctccgtcagt ccatcgagaa tcgctaacgc cttgatcaac caataaaccc cgtaaattgt
    50581 ccggcatttc gtgaggaatg ccgacattta tgctcgccga ccaccgcccc cgttattttg
    50641 tgctatgttt attgaataat gcgctttgct tttaactttt aaagcagaaa tattgcataa
    50701 ttattctgtc aaaggtacta tcttcgacct caatagctat cagactgcca gtatacgagt
    50761 gtcaatgagt attcaattaa acggcattaa ttgcttctac ggcgcgcatc aggcgctgtt
    50821 cgatatcacg ctggattgcc cacagggcga aacgctggtg ttacttggcc ccagcggcgc
    50881 gggtaaaagc tcgctgctgc gtgtactcaa tctgcttgag atgccgcgct ccggtacgct
    50941 caacattgca ggcaaccatt tcgatttcac caaaacaccc tctgacaaag cgattcgcga
    51001 tttgcgtcgt aacgttggca tggtgtttca gcaatacaac ctgtggccgc atctgaccgt
    51061 gcagcaaaac ctgattgaag cgccctgccg tgtactgggg ttgagtaaag atcaggcgct
    51121 ggcccgtgca gaaaaactgc tggaacgtct gcgtctcaaa ccttatagcg atcgttaccc
    51181 gctgcatctt tctggtggtc agcagcagcg tgttgctatt gcccgtgcgt tgatgatgga
    51241 accgcaggta ctgctgttcg atgaaccgac cgccgcactg gacccggaaa ttacggcaca
    51301 aatcgtcagc atcattcgtg agctggcaga aacgaatatt acccaggtga tcgtcaccca
    51361 cgaagttgaa gtggcgcgta aaaccgccag ccgagtggtg tatatggaaa atggtcatat
    51421 cgtagaacaa ggcgacgcga gctgctttac cgagccgcaa accgaagcat ttaaaaacta
    51481 tctctctcac taagattcgg gataacaaca atgaaaaaag ttctgattgc cgcgttaatt
    51541 gcaggtttta gtctttccgc cacagctgcc gaaaccattc gttttgctac cgaagcatcc
    51601 tatcctccgt ttgaatcgat tgatgcaaac aaccagatcg ttggttttga cgtcgacctg
    51661 gcacaagcgc tgtgtaaaga gattgatgca acctgcactt tctctaacca ggcgtttgac
    51721 agcctgatcc caagcctgaa attccgtcgc gtagaagccg tgatggcggg catggatatc
    51781 actccggagc gtgaaaagca ggtgctgttt accaccccgt actatgacaa ctctgccctg
    51841 tttgtgggtc agcaaggcaa atacaccagt gttgatcagc tgaaaggcaa aaaagtcggc
    51901 gtacagaacg ggacgacaca ccagaaattc attatggata agcacccgga aatcactacc
    51961 gttccgtatg acagctacca gaacgcaaaa ctggatctgc aaaacgggcg tatcgacggc
    52021 gtcttcggtg acaccgcagt ggtcactgag tggctgaaag ataacccgaa actggcggcg
    52081 gtgggcgaca aagtgaccga taaagattac ttcggcactg gcctcggcat cgcggtacgt
    52141 cagggcaaca ctgagctgca gcagaaactc aacactgcgc tggaaaaagt gaagaaagat
    52201 ggcacttacg aaaccatcta caacaaatgg ttccagaagt aattcctgat gaatgaattt
    52261 tttcctttag caagcgccgc cgggatgacc gtcggccttg ccgtttgtgc attgattgtc
    52321 gggctggcgc tggcgatgtt ctttgcggta tgggagtcgg caaaatggcg tcctgtcgcg
    52381 tgggcaggtt cagcgctggt aaccattctg cgtggcctgc cagaaattct ggtggtgctg
    52441 tttatctatt ttggctcctc gcagctgctg ctgacgcttt cggatggctt cactatcaat
    52501 cttgggttcg tgcagatccc agtgcagatg gacattgaga acttcgacgt tagcccgttc
    52561 ctttgtggtg tcatcgctct gtcactgctg tatgccgcct atgcctcgca aacgcttcgg
    52621 ggcgcgttga aagcggtgcc ggtgggtcag tgggagtctg gtcaggcgct ggggctgtcg
    52681 aaatcggcta tctttttccg tctggtgatg ccgcagatgt ggcgtcatgc gctgcctggc
    52741 ctcggtaacc agtggctggt gctgctgaaa gataccgcgc tggtcagttt gattagtgtg
    52801 aatgatttaa tgctgcaaac aaaaagcatc gctactcgta cccaggaacc atttacctgg
    52861 tacattgtgg cggcggcgat ttacctggtg atcaccctgc tcagtcagta cattctcaaa
    52921 cgcattgacc tgcgcgcgac acgttttgag cggaggccca gctaatgttt gagtatttac
    52981 ccgaactgat gaaagggcta cacaccagcc tgacgctaac cgttgcctcg ctgattgtgg
    53041 cactgattct ggcattgatt tttaccatca tcctgacgct gaaaacgccg gtgctggtgt
    53101 ggctggtgcg gggttatatc acgctgttta ccggtacgcc gctgctggtg cagatcttcc
    53161 tgatttatta cgggccgggc cagtttccga ctttgcagga gtatccggca ctgtggcatt
    53221 tgttgtcaga accgtggtta tgtgcgctga ttgcgttatc gctgaacagt gcggcgtata
    53281 ccacgcagct gttttacggt gcaattcgtg cgatcccaga aggtcagtgg cagtcctgta
    53341 gcgccctggg aatgagcaaa aaagatacgc tggcgatcct gctgccgtat gcctttaaac
    53401 gctcgctctc ttcttattcc aacgaagtgg tgctggtgtt caaaagtacc tctctggctt
    53461 acaccattac gctgatggaa gtgatgggat acagccagtt gttgtacgga cgcacctacg
    53521 atgtaatggt gttcggtgcg gcagggatta tttacctggt cgtcaacggc ctgctgacgc
    53581 tgatgatgcg tctgatcgag cgcaaagcac tggcattcga acggcgaaat taatgtagct
    53641 gcgtacattc gccggggggg tttgtaggtc tgataagacg cgccagcgtc gcatcagaca
    53701 tcagcacggt gcctgtgccg gatgcggcgt ggacgcctta tccggcctac atagtgcata
    53761 aattcttata atgaagacgg acaactcact aaggtatccg tcttttttta tttcatttaa
    53821 attatttaat catatttatt gcatataaat tcacttgatg gcattgttat cccatgccgc
    53881 agacacggcc aaaaatcata agattgacag acgggagttc catcatgaaa aagttagttc
    53941 ttgccgcttt acttgcttcc tttactttcg gtgcttctgc cgcagagaaa atcaattttg
    54001 gcgtttcagc cacctatcca ccctttgaat ctataggtgc taataatgag attgtcggct
    54061 ttgatatcga tctggcaaaa gccttgtgca aacaaatgca ggcagaatgt acttttacta
    54121 atcacgcgtt cgacagcctg atcccgtcac tgaaattcag aaaatatgac gccgtaatct
    54181 ccggtatgga tatcacgccc gagcgtagca agcaggtatc gtttaccacg ccctactatg
    54241 aaaactcagc cgtcgtgatt gccaaaaaag atacctacaa aacgtttgcc gatctgaaag
    54301 gcaaacgtat tgggatggaa aacggcacca cgcaccagaa atatattcag gatcagcacc
    54361 ccgaagtgaa aactgtctcc tatgacagtt atcagaatgc ctttatcgat ctgaaaaatg
    54421 gtcgtattga tggggtattt ggtgacacag cggtggtaaa cgaatggctg aaaaccaatc
    54481 cgcaactggg cgttgctact gaaaaagtga ccgatccgca atacttcggc accggtctgg
    54541 gcatcgctgt acgtccggat aacaaagctc tgctggaaaa actgaataac gcgctggcag
    54601 caattaaagc tgacggtaca tatcagaaaa tcaatgacca gtggtttccg cagtaattga
    54661 gactgatgac aaaagtaaga ttgcctgatg cgctgcgctt atccggccca catcaactct
    54721 gcaatgtatt gatttagcat gattttgtag gccggataag gcgtacacgc cgcatccggc
    54781 atgaacaaag cgcacctgat cagcaatctg aagccgcacc tgcggctttt ttattgcttc
    54841 accagcagcg tcagcacttc atagtgcgcg gtgtgcggga acatatcaaa aagctgtacc
    54901 cgttcgatgc ggtaacctgg cagttcgcgg atatctttcg ccatggtttg ggcgttacag
    54961 ctggagtaga taataaaacg cggtgccatt gttgagagat aatcacatag cggtttacca
    55021 atgccgcggc gcggcggatt aaccagcacc agctccggca cctccccctg agcggtggca
    55081 aactgagtgg agtccagcgc ctgaaattgc aaacgcgtta agcccagttc agcggctgac
    55141 tgctttgcac aggcaatggc ctctgatgca atttcgatcc cggttaactg catgtcaggc
    55201 gtcgcgcagt gtaaaccaaa gccacccaca ccgcagaaca gatcccacat atgtttaact
    55261 ggcagctgcc gtacccagtc gcgcgcggta gcgtacagct ggctggcgac cgccggatta
    55321 gtctggaaga aactttgcgg acggatccac agcggtacgt cattaaaacg ctctgccagc
    55381 gcctgttgtt cagtaaggta gatctccgtc tccccttcca taatcgccat atgtaccggc
    55441 tgaatattga cggtaataac tttcagttgc ggtagttgtt cctgtaacca cggcagcgcc
    55501 ttacgcagtt gtgccagttt ggtttcagaa cgcagtacaa agcgcagcat catgccgcca
    55561 tcgctctggc tttcagtcag cagaatgtat ttcagttcgc cacgcttacg cgccacgttg
    55621 tagggtgtca gccccgcacg ggcgataaac ggttttagcg ccgcaaaaac gggcgcaaat
    55681 gaggcaggat aaagcgggca gtcacaaagg tcttctggtg tgccgtcgcg atgcagcata
    55741 ccgagcagtg gtttttcaac gctaccactc accaccattt tggctttatt acgaaaccct
    55801 tgttccgggc ctgacaccgg cgcgcaccat tcctcaaccg gaaaatcggc gagcagattt
    55861 ttaagatcgg cggttttagc ggagagttgc tctggaatcg gctgcgttat ccactgacag
    55921 gaacgacagc gacccgcgtc gtaaagtgcg cactgcatac attgaccttc acatcatcag
    55981 gggcgacgat tatacacatt attgcaactg gaagaaccgc cgactggtcg agggaacgaa
    56041 cagcagcatc aggatgagca tatccggcag cttctgcagc atcaggctat ggaagatttc
    56101 acgttttgat tcaccgggaa tgctgaacag ctccggataa ccgtacccca gcgaagccgc
    56161 ccacaagtaa cttgcggcgg tgatttgtgt cagcagatat agccagcgcg cccagcgacg
    56221 ccctttcacc agtgaaaacg cacaccagat ctcaatgaaa accagcacca gactgcttaa
    56281 aaagaccagc gttaaactcc aggtttgtac gctgcgatga atgaactcgc caataccgcg
    56341 cacgcccagc gtattgaaaa tcatcagcac gtcgaggcca cggatcataa taatggcgag
    56401 cgccgccacc tgcaccagcg caggcacatt caggcgagca tgagatgaag atgttttctt
    56461 aaaaaatccc aacgtttcgt cttccatgaa aacaatgccg cggcatgcgc ggcattatgt
    56521 agccaggttg gcaaatttta gtgtcttcag ccatgtcttg cacgctggat atcgcgtatc
    56581 cgctgttttt ctgcgcgagc catcagatac caggcgataa atccgacaat tccgaccgca
    56641 ccgagaatta acgttgcaag ggcgttgatt tccggattca cccccatccg cacgctggaa
    56701 aagaccagca tcggtaacgt ggtggctccc ggcccggaaa caaagctggc gatcaccaga
    56761 tcatcaagcg acaaagtaaa agccagtaac cagccagaaa tgatcgcggg catgatcatc
    56821 ggtagcgtaa tgacgaaaaa cactttcagc ggcgtcgcac cgagatccat cgctgcttct
    56881 tctatcgagc gatccagttc ccgcaatctt gaagaaataa cgaccgccac ataagccgta
    56941 caaaaagtga catgcgccag ccagatggtg agcataccgc ggtccgcagg ccagccaatg
    57001 gcatgagcaa gcgcgacgaa taataacaac agcgacaagc ccgtgatgac atctggcatc
    57061 accagcggcg cggtgatcat aaaggcaaag ccatttgatc cgcgaaacct gccaaagcgc
    57121 accagcacca ccgccgcaat agtcccgagg atcgccgccg ccgttgccgc acaggcagca
    57181 attgtcaggc ttaaaccaac cgcactcatc atcgcgtcat cgcgcaataa ctcaccatac
    57241 cagcgcgttg accagccggc ccacaccgtc accagcttcg agctgttaaa cgaatagata
    57301 accagcatca gcattggcgc gtagagaaag gtaaagccca gcagcaaaat cacaatccgc
    57361 cagggcgaac gaactaccgg taaattattc atccgtgttc tcccacgctt ttttgctggt
    57421 gtttgtggaa ccacattatc ggcacaatta gcagcagcaa catgatgatc gctaccgccg
    57481 aggccaccgg ccagtcgcgg ttattgaaaa actcttgcca tagcacgcgc ccgatcatga
    57541 tgctgtccgg gccaccgagc agttccggga tcacaaactc gcccacagcc gggataaaca
    57601 ccagcatcga tccggcaata atcccacctt tagtcagcgg cacgatcaca gtaaagaacg
    57661 ttttcagcgg tcgtgcaccg agatccagcg ctgcttccac cagcgaataa tcaatacgaa
    57721 tcaacgcggt ataaatcggc agtaccataa acggcacgta ggcgtaaaca atgccaatat
    57781 aaactgccag attggtatga agaatggtca gcggttgatc gataaccccc agccacagca
    57841 gaaaattatt cagcacaccg ttgtttttta atattcccat ccaggcataa acgcggatca
    57901 gaaacgaggt ccacgacggc aggatcacca gtagtaataa aatattacgg gtcgaaggct
    57961 tactgtgcgc caccgcccac gccagcggat agccgatcag taaacagcaa aatgtcgaaa
    58021 tcgcagccac ctgtagcgac tggagataag catcgaaata gagcggatcg tcggtcagtt
    58081 gcagaaaatt accgagatta agagtgatgg acagttgccc gtcagcccac tccatcagtt
    58141 cggtataagg tggaatagcg cgcgccatct ccgccaggct tattttaaag acaatcagaa
    58201 atggcagcag aaacagcaag atcaaccaga tatatggcaa cgcaatgacc agtttgcgcc
    58261 catgcttcat ttgcagctgc gacaaccaca gcttaaatcc gcccggcggt ttcgactggg
    58321 cagcaggttc aagtgtattc atctttgctc cttaaaccgt cagcaccaca cagctgtcca
    58381 cttcccagca caaacgcact tcgtcgcccc aggtcggtaa ccctttacga tgacgatggg
    58441 cgttttgtag ctgggcgcta atcatctgcc cacttttcag acgaacgtga tacaccgaaa
    58501 gatcgccgag ataggcaatg tgtatcacct cccccaccgc gaagttacaa ccattggcgg
    58561 gcggctcttc gcaaagcatg attttttccg ggcgcagcgc tacgtgtacc ggcacgttat
    58621 cgaccactga agcatccgca tcgactttca gtggatgcac cagccccggc gaatcaagca
    58681 ccaggccatc ttcctgacgc tctttgagta caccttcaaa gacattaacc gaaccgataa
    58741 attcagcgct gtagcgggta gttggatgct cgtagatctc ttccggctcg ccaatctgga
    58801 caaatttccc gcgattcata atggcgatac gccccgccat ggtcatcgcc tcttcctgat
    58861 cgtgggtgac catcacacaa gtcacaccga cgcgctccag aatatccacc acttcaagct
    58921 gcatccggtc acgcagcttt ttatccagcg cgcccatcgg ctcatcgagc agtaatagtt
    58981 tcgggcgttt ggcaaggctt cgggccaggg ccacacgctg tcgctgacca ccggaaagct
    59041 gatgcggttt gcgtttggcg aactcctgca tgtgcaccag cccaagcatc tcgttgaccc
    59101 gggtggcaat ttccgctttc ggtagtttgt cctgtttcag gccaaaggcg atgttctgct
    59161 ccacagtcat atggggaaac agcgcgtaag actgaaacat catattgatg gggcgcaggt
    59221 aaggcggcac ctgtgacaaa tcgacgccat caagcattat ctgtccggca gaaggttgtt
    59281 cgaaacctgc cagcatacgc agcagcgtgg acttgccaca gccggatgcg cccagcagcg
    59341 cgaagatttc acctttgtag atggtcaggc tgacatcatc caccgcatgt tgaccatcgt
    59401 aggatttggt gaggttgcgg atttctaata gcggcgtcag cgccttacgg gttttcgcct
    59461 gcgggcgagg gatagcgtca ttcacggctg ctctccggca ttgaatgtgt gcaagcgcac
    59521 catgatggtg cgaattgccg gcgggtgtgg tgcgcccgcc cggcatctaa ggctgcggat
    59581 tattttccgc tcttcacttt ggtccacgcg cgggtgcgca cacggtcgat tttcgggtcc
    59641 tgcactttca gagtgaacag cttcgcacga acatccgcag gcggataaat acccgggttc
    59701 tcacggactt ccgcactcac cagcggcgtg gctgctttat tggcgttggc atagaacaca
    59761 ttgtcggaaa tatgcgctac tacatccggg cgcagcaggt aattcaggaa ctgataggct
    59821 tcgtctttgt ttttggcatc cgcaggcatg gcgaatacat caaagaacgc catcgcccct
    59881 tcttttggaa tcgagaacga gacattcact ccattcttcg cttccttcgc gcggtttgac
    59941 gcctgccaga catcacctgc ccagccgata gcgacgcaaa tatcgccgtt tgccaggtcg
    60001 ttaatgtatt gagatgaatg gaaataacga atgttcgggc gcagctttaa cagcagatcg
    60061 gttgccggtc cggtgtaatc atccgcttta gtgctgttgg gatctttgcc gagataattc
    60121 aacacggtag caaaaacttc ttctggcgca tccaggaaag agacaccgca gcttttcagt
    60181 ttttccagat tttcaggttt gaggatcaag tcccagctat cgacgggcgc gttttcgccc
    60241 agcacagctt taactttatc aacgttatag ccaatcccgg tcgtcgccca catatagggc
    60301 atagcaaatt tattgtcggg atcgtgtttg gcgaccagct tcagcagctc cgggtcgaga
    60361 ttcttccact ccggcaattt gcttttgtcc agcggctgga aaactcccgc agtcaactgg
    60421 cgttcaagaa agctggcaga tggaaccacc aagtcaaagc cggtgctacc cgccattaat
    60481 ttgccttcca gtacttcgtt agagtcgaaa acatcgtaga cgactttaat accggtttct
    60541 ttttcaaaat tggccaccgt gtccggggcg atataatcag accagttata aacgtggagt
    60601 gttttttgtt cagccgcgag cgtgccgaca gagacggcca tcagagcacc cgcagccaga
    60661 cccgaaagcc attttttatt taaggcggtc atatctcttt ccttctgaaa gttcgttaac
    60721 aaacgaacaa aagttgtcac actaaaagat atgcaataaa cgtgcatatt ttttatcggc
    60781 ggggtgtaag aaaagagaac tctctcccgg ccagcactgc tcgctatata aaagcatcgc
    60841 aagaataact ttagccaggg tttgagacta tagctcggat taaaaaacgg tatttaacaa
    60901 atttttatgc gtatttgctc tatgtgataa agccaaatcg acacaagaga agtgaaataa
    60961 tctttaagaa aaggttaaaa acagcagata attttgcagc gcgcagccac tgcaccagcg
    61021 actgcgtgct cgaaaggagt tatcagtgga ggaaatagtt ttcggtaacg ttgttttgcc
    61081 cttcatcgtc agtcggtaac agcagttgat gggcattagc ttcaagaatg accatcgaaa
    61141 tctgctcttc gctctgacgc acgaaccatg caaactgctc ataggttacg ccctgcataa
    61201 cggataaaga ctggcaaacc accagctttg gcagattatc atcctgcata tcaagaaatg
    61261 ctttcacggt cagcgaactg gcattgatgg cagataaatc cgccgccagc ggcaatacgg
    61321 ctgaaggtcg gacttccgcc atggcagaaa acaggatcgt gttatcgatc agatcgattt
    61381 tggcatcaaa gacaccgtcg aaattctgca tatggggcag atgcagagcc tgacagttat
    61441 cacattcaaa aaaactcatc cccaggtcat cgagccattg acgcagcgta tccagaccag
    61501 gaacgaccag cgatgtcata attttgtaca acctctttcg ataaaaagac cggcacagct
    61561 tacgcaaaaa gcgcaggcaa aaccatgatc agtaatgtga ttgcgactaa ccacccgttt
    61621 tcaggcaata ttctgtcgta gcgtggcgtt cgatccagcg gatcatttta cccgcgatgt
    61681 cgataccggt ggttttttct attccttcca gccccggcga cgcattcacc tccatcacca
    61741 acggcccgcg attagcacgc agaatatcca caccagcaac gtccagcgcc atcgttcgcg
    61801 cggcttttat cgcgatttca cgctcctgtg gtgtgatact ggcgacgctt gccgcgccgc
    61861 cacgatgcaa attggaacga aaatcgccct ctttcgcccg ccgttcaatc gcagcgacca
    61921 cttcatcgcc aacaaccaga cagcggatat cgcacccttg cgcctctttg atatattcct
    61981 gcaccagaat atgcgcgttc agaccgcgga aagcgtcaat cacgctttcc gccgcctgac
    62041 gcgtctccgc cagcacgacg ccaattccct gcgtgccttc aaccaacttg accaccagcg
    62101 gcgcaccacc gaccatgtcg attaaatcgc tggtatcatc cggcgaatgc gcaatgcccg
    62161 tgacaggcag gtcgatgccc tgacgcgcca gcagttgcat ggaacgcaat ttgtcacgcg
    62221 cccgggcaat ggcgaccgac tcattgagcg gatagctccc cagcatctcg aactggcgca
    62281 gtgccgccgt cccataaaag gtaatggcgg tgccaatacg cgggatcact gcgtcaaaat
    62341 ggggtaactt gcggcctttg tagtgaatag aagacgccgc aggatttatg ttcatgtagc
    62401 aagaaagcgg atcaagaatt tcaaccaggt gaccgcgctg tatagcggct tcacgcagcc
    62461 gcttacacga atagagcgtt ccatcccggg acaatatggc aattttcacc ctgcacctct
    62521 ctgtcagacc tggtcaaaag cccgcgtatc atacatcgac gtggcggttt tagcgcgtcg
    62581 cccaaccctg tttgtgcaaa taatccagaa taaatgggcg gctttcttta atgattgttc
    62641 ggcggatatg atcgctccag gtatcccggc gattattgct gccacgggtg aggtaatatt
    62701 ccgccagttg ctcgtcatac tgcgccagtg cgcctttatc cagcggttga tagctgtttt
    62761 catgcaccaa aatggaggcc ggtaaacgcg gcttaagatc cggattatcc gcaggccagc
    62821 caaggcacag cccaaacagc ggcagaacat gctgcggtaa tttaagcagt ttcgtcaccg
    62881 cttcaatatt attgcgcagg ccgccgatat ataccccgcc caatcccagc gattccgctg
    62941 cgattaatgc attctgcgcc atcattgccg tatcaacgac accgagcaac agttgttccg
    63001 ccaggccgag ctgagcatcc ggacagatct gtaaatggcg gttaaagtcg gcacagaaca
    63061 cccagaactc cgccgcttgc gctacgtgtt tttgcccgcc ggtcagcgtc accagttctt
    63121 cacgtaacgc tttgtcggta atgcgaataa tgctactgca ctgcaaaaaa ctggaactgg
    63181 acgtcgcacg ggcgctgtta ataatcgcct cacgctgcgc ttcggaaatg ggttcatcag
    63241 tgaaatggcg aatggagcga tggccacaaa taagttcaat ggttggcgtc attatctttt
    63301 tctctttctg aacgtgaata ttgcggtgga cggttcatca gctgtggggc aagacgtttt
    63361 gccacctgaa gaataaccac caccgcagcg ggaagcatga gcaaaacacc gagaaaaatc
    63421 atcagaatct gcacttctgg ccgagaaaat ggctcaggca gcgacaggga gtcgcttacc
    63481 gacagcagcg ccaccgccag tagcatcatt ccgataaatt ccagtatcaa cacgccttta
    63541 ggcaatttac cgatcgcgcg catacgcttc cctctgcaaa gtgagccttc agtctaaaac
    63601 ttttcactgt attgtgttta acagttatag cttttagcaa ttaatgcaac aggttaaacc
    63661 tactttcagc gaatacattt tagcgtgatc attacaggca taaatctatg aggagagaaa
    63721 taatgcaaac cgttattttt ggtcgttcgg gttgccctta ctgtgtgcgt gcaaaagatc
    63781 tggctgagaa attgagcaat gaacgcgatg attttcagta tcagtatgta gatattcgtg
    63841 cggaagggat cactaaagaa gatctacaac aaaaggcagg taaacccgta gaaaccgtgc
    63901 cgcagatttt tgtcgatcag caacatatcg gcggctatac cgattttgct gcatgggtga
    63961 aagaaaatct ggacgcctga tcgtctgaca agccctcgcg ttgagggctt tactgatttt
    64021 ttctgtgctg tggtttaaac aaactactga taaataagaa acacagtgcc cccagcgcac
    64081 accagaacac cgcgcttagt aaccatgcca gctcttgcca gaatgagcgc gtcggtgaaa
    64141 aaaacagccg cataatgagc attgaacagg gtgccgccag cattgcgcca aatagaggtt
    64201 tcaggacttc tctacgctgt gaaaagaagc tggcgactgc tccaggaaga atgaaaaata
    64261 gcaagccgat ttcaggatgc ccggcagccc gaaaagcgcc tttcatgtgc gtcgccagaa
    64321 aaaggcacac cacaatgaag aggacaaaac agcagattgc ccccgcccaa cgttgtttat
    64381 gtttcactcg ttcctcctga cactgcgtct atcgaacaca tttttcgcca gtgtggcgtt
    64441 cagtaagata aagccgcttc gcattccatg ctaatatagg ccaacgcaat tcatatagcc
    64501 gttgatacc
//
LOCUS       DAZFRJ010000005        62884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000005 DAZFRJ010000000
VERSION     DAZFRJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.005"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKUN_000268"
     CDS             complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHKUN_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO6658595.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKUN_000269"
     CDS             complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHKUN_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO6658596.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKUN_000270"
     CDS             complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHKUN_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO6658597.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKUN_000271"
     CDS             complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHKUN_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO6658598.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKUN_000272"
     CDS             complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHKUN_000272"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO6658599.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKUN_000273"
     CDS             complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHKUN_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO6658600.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKUN_000274"
     CDS             complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHKUN_000274"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO6658601.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKUN_000275"
     CDS             complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHKUN_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO6658602.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKUN_000276"
     CDS             complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHKUN_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO6658603.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKUN_000277"
     CDS             complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHKUN_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO6658604.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKUN_000278"
     CDS             complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHKUN_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO6658605.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13704..13780)
                     /locus_tag="ACHKUN_000279"
     tRNA            complement(13704..13780)
                     /locus_tag="ACHKUN_000279"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13744..13746),aa:Met,seq:cat)
     gene            14128..15471
                     /gene="argG"
                     /locus_tag="ACHKUN_000280"
     CDS             14128..15471
                     /gene="argG"
                     /locus_tag="ACHKUN_000280"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO6658606.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHKUN_000281"
     CDS             complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHKUN_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6658607.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17563..17649)
                     /locus_tag="ACHKUN_000282"
     tRNA            complement(17563..17649)
                     /locus_tag="ACHKUN_000282"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17613..17615),aa:Leu,seq:gag)
     gene            complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHKUN_000283"
     CDS             complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHKUN_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO6658608.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHKUN_000284"
     CDS             complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHKUN_000284"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO6658609.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHKUN_000285"
     CDS             complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHKUN_000285"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO6658610.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHKUN_000286"
     CDS             complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHKUN_000286"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO6658611.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHKUN_000287"
     CDS             complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHKUN_000287"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO6658612.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHKUN_000288"
     CDS             23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHKUN_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO6658613.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHKUN_000289"
     CDS             complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHKUN_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO6658614.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24454..25887
                     /gene="dacB"
                     /locus_tag="ACHKUN_000290"
     CDS             24454..25887
                     /gene="dacB"
                     /locus_tag="ACHKUN_000290"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6658615.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHKUN_000291"
     CDS             complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHKUN_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO6658616.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHKUN_000292"
     CDS             complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHKUN_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6658617.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHKUN_000293"
     CDS             complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHKUN_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO6658618.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHKUN_000294"
     CDS             complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHKUN_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO6658619.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29055..30026
                     /gene="ispB"
                     /locus_tag="ACHKUN_000295"
     CDS             29055..30026
                     /gene="ispB"
                     /locus_tag="ACHKUN_000295"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO6658620.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHKUN_000296"
     CDS             30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHKUN_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO6658621.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHKUN_000297"
     CDS             complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHKUN_000297"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO6658622.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHKUN_000298"
     CDS             complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHKUN_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO6658623.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHKUN_000299"
     CDS             complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHKUN_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO6658624.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHKUN_000300"
     CDS             complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHKUN_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO6658625.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHKUN_000301"
     CDS             complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHKUN_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO6658626.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHKUN_000302"
     CDS             complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHKUN_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO6658627.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHKUN_000303"
     CDS             complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHKUN_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO6658628.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHKUN_000304"
     CDS             35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHKUN_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO6658629.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHKUN_000305"
     CDS             36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHKUN_000305"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO6658630.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHKUN_000306"
     CDS             37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHKUN_000306"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO6658631.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38180..38755
                     /gene="lptC"
                     /locus_tag="ACHKUN_000307"
     CDS             38180..38755
                     /gene="lptC"
                     /locus_tag="ACHKUN_000307"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO6658632.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38724..39281
                     /gene="lptA"
                     /locus_tag="ACHKUN_000308"
     CDS             38724..39281
                     /gene="lptA"
                     /locus_tag="ACHKUN_000308"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO6658633.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39288..40013
                     /gene="lptB"
                     /locus_tag="ACHKUN_000309"
     CDS             39288..40013
                     /gene="lptB"
                     /locus_tag="ACHKUN_000309"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO6658634.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHKUN_000310"
     CDS             40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHKUN_000310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO6658635.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41517..41804
                     /gene="hpf"
                     /locus_tag="ACHKUN_000311"
     CDS             41517..41804
                     /gene="hpf"
                     /locus_tag="ACHKUN_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO6658636.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHKUN_000312"
     CDS             41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHKUN_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO6658637.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHKUN_000313"
     CDS             42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHKUN_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO6658638.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43310..43582
                     /gene="npr"
                     /locus_tag="ACHKUN_000314"
     CDS             43310..43582
                     /gene="npr"
                     /locus_tag="ACHKUN_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO6658639.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHKUN_000315"
     CDS             43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHKUN_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO6658640.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHKUN_000316"
     CDS             complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHKUN_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO6658641.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHKUN_000317"
     CDS             complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHKUN_000317"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO6658642.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHKUN_000318"
     CDS             complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHKUN_000318"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO6658643.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHKUN_000319"
     CDS             complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHKUN_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO6658644.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50069..54529
                     /gene="gltB"
                     /locus_tag="ACHKUN_000320"
     CDS             50069..54529
                     /gene="gltB"
                     /locus_tag="ACHKUN_000320"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO6658645.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54542..55960
                     /gene="gltD"
                     /locus_tag="ACHKUN_000321"
     CDS             54542..55960
                     /gene="gltD"
                     /locus_tag="ACHKUN_000321"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO6658646.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56144..57191
                     /gene="yhcG"
                     /locus_tag="ACHKUN_000322"
                     /pseudo
     CDS             56144..57191
                     /gene="yhcG"
                     /locus_tag="ACHKUN_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57251..57715)
                     /gene="nanQ"
                     /locus_tag="ACHKUN_000323"
     CDS             complement(57251..57715)
                     /gene="nanQ"
                     /locus_tag="ACHKUN_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO6658647.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57712..58587)
                     /gene="nanK"
                     /locus_tag="ACHKUN_000324"
     CDS             complement(57712..58587)
                     /gene="nanK"
                     /locus_tag="ACHKUN_000324"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6658648.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58584..59273)
                     /gene="nanE"
                     /locus_tag="ACHKUN_000325"
     CDS             complement(58584..59273)
                     /gene="nanE"
                     /locus_tag="ACHKUN_000325"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO6658649.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59321..60811)
                     /gene="nanT"
                     /locus_tag="ACHKUN_000326"
     CDS             complement(59321..60811)
                     /gene="nanT"
                     /locus_tag="ACHKUN_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO6658650.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60921..61814)
                     /gene="nanA"
                     /locus_tag="ACHKUN_000327"
     CDS             complement(60921..61814)
                     /gene="nanA"
                     /locus_tag="ACHKUN_000327"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6658651.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61936..62727)
                     /gene="nanR"
                     /locus_tag="ACHKUN_000328"
     CDS             complement(61936..62727)
                     /gene="nanR"
                     /locus_tag="ACHKUN_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6658652.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15393 a        17089 c        15524 g        14878 t
ORIGIN      
        1 ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc
       61 tgattactga tacaccggca gtaaattaaa gctcgataaa atatggacca gtgcgttgcc
      121 aacgccaaac accagaatca gcgtaatcat cggcttgccg ccccagacgc ggaatttcgg
      181 gctgccaaag cgtttacgcg atgcgcgggc taacagcgcc ggaacaattg ccgcccagat
      241 ggtagctgct aaaccagcat aaccaatggc gtacaggaat ccgttcggga acagtagacc
      301 acccacaaca ggcggggcaa aggtcagcaa tgccgttttc aagcgcccca gggctgagtc
      361 atcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga
      421 actcgctacc gcaaagtttg agaacacgac cagcagaagg tccaggctac ggctgttcag
      481 cacgccgctt aatgcctgta ccagcacatc aatattaccg cccttctctg caataccgat
      541 aaactccgga cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag
      601 tgccatcagc gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata
      661 cttcatcagg cttggcacgt taccgtgata accaaacgac gccaggcaga acggcaatgt
      721 catcagcaga tacggcgcat aagatgcatt gctttctgca acgttgaaca atgtcgcagg
      781 ctgcacatgc cccaacaggc tgccaaaggt gaggaagaag gtaatgactt tcgcccccag
      841 cacaatcgcc gtcatgcggc tgacggcctt agtactcaac cacaccacaa acgctaccag
      901 cagtgcaaaa ccaaaacccg ccgcccgtgc cgggacgttt agcgacatct ctgcgaaggt
      961 gtgatgcaga attgaaccac tggcagaaat ataggcatag gtcaggatat agagcacaaa
     1021 ggcaatggaa ataccgttga ccacgttcca gcctttgccc agcaaatctt tggtgatggt
     1081 gtcaaaactc gaaccgattc ggtaattcag gttagcttcc agaatcatca agccggaatg
     1141 cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaaaaacc acgccccgga
     1201 catgaccact ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac
     1261 gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct
     1321 ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac tgtaccagta cacgagtaca
     1381 aaagacagaa aaaaagcccc gatggtaaaa atcggggctg tatatattat tttacagatt
     1441 gtgttcgctg ttcagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc
     1501 atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg
     1561 gccaccttca cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccaccgaa
     1621 accacgacca ccgccacgac gctcaccgcc agtatgcggc tgcgcatcgc ccagcaactg
     1681 catattcatc ggcttgttca gaatgcgagt acgcgtaaag tgttgcagta cttcacccgg
     1741 catacctttc ggcagttcga tagtggagtg agaagcaaac agcttaatgt taccgatgta
     1801 acggctgctg atgtcgcctt cgttagcaat cgcgccaacg atatgacgaa cttcaacacc
     1861 atcatcgcgg cccacttcaa tgcggtaaag ctgcatgtca ccaacatcac gacgttcacg
     1921 acgcggacga tcttcacggt cgccacgtgg gccacggtcg ttacgatcgc gcggaccacg
     1981 gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag
     2041 agtacgttca ccctgtgcca ttttcagcag tgccgcagcc agagtttcga gatcctgctc
     2101 ttcaccttct gcagtcggct gaattttgct cagcagtgcg cggtattgat ccagatcgct
     2161 gctttccagc tgctgctgta ctttagcggc gaatttttcc agacggcgtt tgcccagcag
     2221 ttctgcgttc ggcagttcta cttccggaat agtcagcttc atagtacgtt caatgttgcg
     2281 cagcagacga cgctcgcggt tctcaacgaa cagcagcgcg cggccagcac gacccgcacg
     2341 accggtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac
     2401 taccaggctg atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat
     2461 gtccagacga ccatctttca ggcgttccag tgtctgttca cgcagcgcct ggttcatgtc
     2521 accgttcagc gcggcgctgt tgtagccgtt acgctcaaga gcttcagcca cttccagagt
     2581 cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg
     2641 taccagtgct tcgtttttgc gcatacccca gacagtccag tagctctggc tgatgtcagg
     2701 acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt
     2761 aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg
     2821 gatctgcgcc ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc
     2881 ttcatccaga accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc
     2941 cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg
     3001 cacgtcataa cgctggccgc cgtatagagc aaccacattt acgccgcgca tgtgtttaga
     3061 gaaatccgtc attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag
     3121 aatctgtggt gctttcagct caggatcaag attctgcaac agaggtaaag agaatgctgc
     3181 agtttttccg ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg
     3241 aatacactct gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag
     3301 gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt
     3361 gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt
     3421 tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc
     3481 cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg
     3541 tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat
     3601 cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg
     3661 ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga
     3721 tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt
     3781 tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat
     3841 ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg
     3901 agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga
     3961 tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc
     4021 gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca
     4081 aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt
     4141 cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca
     4201 tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg
     4261 gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt
     4321 accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct
     4381 gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg
     4441 aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc
     4501 gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct
     4561 gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga
     4621 tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc
     4681 tcaacgcgtt tgtcagcgat ttgagagatg tggaccagac cttctttacc gccgccgatg
     4741 gcaacaaatg cgccaaagtc aacgatacgg gtcactttac cagtgtagac gcggcccact
     4801 tcgatttctg cagtgatctc ttcgatacga cgaatagcat gtttcgcttt ctcgccgtcg
     4861 gtcgctgcga tcttcacagt accgtcatct tcgatttcga tggtagtgcc agtttcttcg
     4921 gtcagggcac ggataacaga gccgccttta ccgataacgt ctttgatctt gtccgggttg
     4981 atcttgatgg tatggatacg cggtgcgaac tcagagatat cgccacgcgg cgcgttgatc
     5041 gcctgttcca ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagcgcaacc
     5101 tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccc
     5161 tcgcgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg
     5221 tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg
     5281 gcagctttga tcggcacacc tgcgtccatc agcgccagag acgcgccgca cacggaagcc
     5341 atagaagagg aaccgttgga ttcagtgatt tcagacacaa cacgtacggt gtacgggaat
     5401 ttgtccatat ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt
     5461 tcacgacgct tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg
     5521 tagtggaaca ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca
     5581 cgagcagtac ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca
     5641 gaaccgtgag tacgcggcag cacgccagta cgcacatcca gaccacggat catatctttt
     5701 tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg
     5761 atagcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc
     5821 gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg
     5881 atgcggtaag catcgctcag gcgagcttca gccagtgcag caacgcgcgc gttcagcgct
     5941 tcgtttaccg gctccggctg ccagtcccaa cgtggtttac cggcttcttt caccagttca
     6001 ttgatgttct gaataacaac ctgctgttgt tcatgaccga acactactgc gcccagcatc
     6061 tggtcttcgc tcagcagttc agcttcagat tcaaccatca gaacagcggc ttcagtaccg
     6121 gcaacaacca gatccagttt gctctctttc agctcgtcct gagtcgggtt cagtacgtac
     6181 tggtcattga tgtaacctac gcgagcagca ccaatcggac cattgaacgg aataccagac
     6241 agagacagtg ctgcggaagc accaatcatc gcgacgatat ccgggttaac ttgcgggtta
     6301 acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg
     6361 cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca
     6421 cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg
     6481 gtcagtggga agaagtcctg acctggtttg gcttttttct ggccaacaac ggtaacaaat
     6541 accgcggtgt catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg
     6601 gtttccagag tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat
     6661 gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat acccgatctt
     6721 ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca
     6781 ttagccgcgc gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag
     6841 tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc
     6901 aagaattagc gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta
     6961 cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg
     7021 ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc
     7081 agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa
     7141 acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat
     7201 gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa
     7261 tttacgcgac gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta
     7321 acattacgcc gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat
     7381 ttcgcccata ccgataaatt tgccgttctc accttctgta acgcgaacca gtccttccag
     7441 cggcgcaccg gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg
     7501 aagattcacc accgggtagt ccgaagctgg actgtccatt ggcatcagta atggatcaag
     7561 taactccgca gccggaatat cctgctgttc ggcttgttcg accagttcac gcagatgttc
     7621 cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca ggtaaataac
     7681 atgcgcgcca caaccgagtt tttcacccag gtcatcaatg atggtgcgga tataagtgcc
     7741 ttttgagcag tgaatttcca gctccagctc attgccttcg tggcgaataa acagcaattc
     7801 ataaacggta atcgggcgcg cttcacgcgg tacttcaatg ccctgacgcg catattcgta
     7861 cagtttttta ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc
     7921 acggaaagta tccagcgccg ccgccagttg ctctgcgcta aaggttaccg gacgttcttc
     7981 aacgatttgt ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg
     8041 atagcgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat
     8101 tggcaacatg ccagttgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt
     8161 ataaatccgt ttcacttttt gcagcgcatc attgctggac ataccctgcg atttatccag
     8221 caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg
     8281 ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt
     8341 gacatgcgca tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata
     8401 cgcaggcgca tcgctttccc cagcaggctg cggatgaaac cagaagcttc ttgcaacgct
     8461 ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg
     8521 gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccaggcga
     8581 ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg
     8641 cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt
     8701 agcccctttt taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc
     8761 gccagcgtca catcaggcaa tccatgccgg atgcggcata aacgccttat cccgcatgga
     8821 accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac
     8881 atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca taccgttacg
     8941 gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac
     9001 cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat
     9061 acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt ccgccagacc
     9121 gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc
     9181 aatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt
     9241 acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc
     9301 cagggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac
     9361 ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tccacagaac cctgtacgtc
     9421 tgccttcagg acgatgttca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt
     9481 ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata
     9541 cagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc
     9601 cggtacgccg gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc
     9661 ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac acagaacgat
     9721 atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt
     9781 atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc
     9841 cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgatgccgg tacccgcttt
     9901 cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga tgccatactg
     9961 ggagagttcg ttcttaacac gatccggatc ggcttctggt ttatcaatct tgttcactgc
    10021 aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg tctgcggcat
    10081 cacgccgtcg tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgtgc
    10141 acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc
    10201 agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggcaac
    10261 tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat
    10321 ggtcacaacc ggcgcgcgcg gttcagccgc agcacccgtg tcacggtcgc tcattaccgc
    10381 ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag
    10441 ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat
    10501 cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat
    10561 ggtttcgccg atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg
    10621 cagggaagaa cctttacgtt ttccgccttt accgccacgt actgctgcgc gtgcttcttc
    10681 acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt
    10741 acgaccacgg ccacgaccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc
    10801 atgttgagaa gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt
    10861 ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc
    10921 gagtttacga cgcgcttctt cttcagcttt acgcttgagc tctgcagctt cctgctcacg
    10981 gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc
    11041 tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc
    11101 ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc
    11161 ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag cctcttgcgg
    11221 atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc
    11281 accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg
    11341 gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga
    11401 gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga
    11461 ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc
    11521 tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc
    11581 tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc
    11641 gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctat
    11701 cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt cttcctgggc
    11761 ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc
    11821 atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt
    11881 cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt
    11941 gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt
    12001 catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccgttacg
    12061 gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc
    12121 atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa actgcgccgg
    12181 gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag aaaccgcctg
    12241 aacacgcgcg ccacgcatac ctacacaggc acctaccgga tcgatacgtt tatcgttggt
    12301 tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa tttcaatcac
    12361 ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt ccggcttgga
    12421 acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg
    12481 aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca ggatcacggc
    12541 ttcagcattg ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc
    12601 ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc
    12661 acgcactttc tgcacgataa cctgttttgc cgtctgggta gtgatacggt caaaggtaac
    12721 agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt cataacgtgc
    12781 ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg
    12841 gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga tctcttgttc
    12901 atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg
    12961 aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc
    13021 gggcttttca cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt
    13081 actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac
    13141 cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga gaaccagagt
    13201 cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc
    13261 cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat ccagcacagc
    13321 acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat
    13381 atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc
    13441 cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc
    13501 cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa
    13561 aaccccgata aatcggggct ttatataact gaaccctata accgcaactg cggtctggag
    13621 cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt
    13681 tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg
    13741 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt ggcaaattct
    13801 actcgttttg ggtaaaagat gcaaatactg ctgggatttg gtgtaccgag acgggacgta
    13861 aaatctgcag gcattatagt gatccatgcc acattttgtc aacgtttatt gctaataacg
    13921 tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc tgctctccct
    13981 taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata aaagatgatt
    14041 aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc aggctcatca
    14101 tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct cccggtaggt
    14161 caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact gctgtggatg
    14221 cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc agacgaagag
    14281 gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc acgtctgatc
    14341 gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg cgcatttcat
    14401 aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc cgtgactggt
    14461 accatgctgg ttgctgcgat gaaagaagat ggcgtgaata tctggggtga cggtagcacc
    14521 tataaaggaa acgatatcga acgtttctat cgttatggtc tgctgaccaa tgctgaactg
    14581 cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg ccgtcatgag
    14641 atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga aaaagcctac
    14701 tccacagact ccaacatgct tggtgcaacg catgaagcga aggatctgga atacctcaac
    14761 tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat tctgggatga gagcgtgaag
    14821 atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc gctgaacggt
    14881 aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg cggtcgtcac
    14941 ggcctgggca tgagcgacca gattgagaac cgtatcatcg aagcgaaaag ccgtggtatt
    15001 tacgaagctc cggggatggc actgctgcac attgcgtatg aacgtctgtt gaccggtatt
    15061 cacaacgaag acaccattga gcagtatcac gcgcatggtc gtcagttggg ccgtctgctg
    15121 taccaggggc gttggtttga ctcccaggcg ctgatgctgc gtgactctct gcaacgctgg
    15181 gttgccagcc agatcactgg tgaagttacc ctggagctgc gccgtgggaa cgattattca
    15241 atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac gatggaaaaa
    15301 ggcgactcgg tgttctcgcc agatgatcgt attggtcaat tgaccatgcg taatctggat
    15361 atcactgata cccgagagaa actttttggt tatgccaaaa ctggcctgct ctcctcctct
    15421 gccacttcag gcgtgccgca ggtggagaat atggaaaaca aaggccagta attcgacatc
    15481 aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc tttctgagca
    15541 tcacataatc gaaaacatca tggtttgcat ccacaacccg tgagtctcct ttatagtgtg
    15601 caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc cacgcattaa
    15661 taaggtaatt attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga tccacgccat
    15721 ccccaagaac ggggctatac cagataaaca tcgggacatg atatgcctgc tggctagcct
    15781 ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg ccgtggtcgg
    15841 caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga cctagcaaac
    15901 tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc ggttgaaaca
    15961 cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa
    16021 caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca
    16081 attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg
    16141 ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt
    16201 gattagccat attaataatg ttgtcggggt aattatgaat gtcatgactc aaaacagaat
    16261 cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcgccgctt attgcttgat
    16321 taaacagttt gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc
    16381 catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa
    16441 tacctgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc
    16501 tttgatgctc tttcgctgct aaagcaaaat agttgagatt gaaaaacggc gtataggtag
    16561 caaatcgcga cgctagtata tatggactga acgctttttt attttttgca tctttataag
    16621 caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca
    16681 ctttttttgt cggcaaggaa acatcatatt ttattattac tgccaaaaaa agaagggata
    16741 aaaaagcaaa ggcacataga taagggatat acatccccag cattttgaca acttcatccg
    16801 gatcactctg gagcacacta atcgcaaatc catcattgaa agttgtacca aaggtacacc
    16861 atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa
    16921 gcaggcgaag cggaagggat gatatacgtt ttacaaccag gatggtcagg acgaagaaca
    16981 gcaatcgctg aaccatatgc gcgccggaag aggcgactaa gttcgtaata ccaaaaacaa
    17041 taacacaaag atacaacaac caatgagatt taaaacttct agcaaatttg ttgaatactg
    17101 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
    17161 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
    17221 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
    17281 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
    17341 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
    17401 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
    17461 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
    17521 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
    17581 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
    17641 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
    17701 gctggttgag tttgttcggt tttcaccggt gcactcagat tttcccattc gctaccttta
    17761 ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa taacgttgcc
    17821 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
    17881 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
    17941 agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc gtacataatc
    18001 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
    18061 aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
    18121 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
    18181 aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg gcttttactg
    18241 catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct tccaccatca
    18301 cgcgaattaa cggttcggtg ccggatttac gcagcaacac gcgtccacgg ttgcccagcg
    18361 cagcttcaac ctctgcggtc acggctttaa ctgactcatg ctcaagtgga tcgccgctac
    18421 ctgcggtgta acgtacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
    18481 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
    18541 cgtcaccggt agtagtttta tccagtagga tcacatgacc ggaattctct gcaccgatac
    18601 gccagccttt ctcctgcatt ttttccagta cgtagcggtc gcccactttc gcgcgcgcaa
    18661 atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc aatgtaccca
    18721 cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
    18781 gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccatcgccgt
    18841 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
    18901 cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg atagcgataa
    18961 cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac
    19021 aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa
    19081 actcgatata gcgacccgcg gcatcaacga tacggctggc tttacccagt tctgccgaat
    19141 caacgcagct gatctccttt tccatttccg cttcgatggc ctcttctacc gcatccggca
    19201 gtttggtgcc gtcgatagag aagaatttaa tgccattatc gtagaacggg ttatgagatg
    19261 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
    19321 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
    19381 ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc ttacgggagc
    19441 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
    19501 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
    19561 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
    19621 tctttgacgt catgaacacg aatgatgtgc gcgccttgca ttgcggcaat gaccgcacag
    19681 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
    19741 tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
    19801 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
    19861 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
    19921 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
    19981 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
    20041 gaaagggagc ggatatcatt aataatgtga gcgccaactt tcgctgactc acggatgact
    20101 tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
    20161 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
    20221 gactcgccac caacgtcaat gatcgtcgcg ccagcattga tcatcagatt cgcatgtttc
    20281 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
    20341 aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc aaagagtttc
    20401 atgatgttat ccctagtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
    20461 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
    20521 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
    20581 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
    20641 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
    20701 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
    20761 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
    20821 gtttcgcttt cgctacgcta cggccgagga atacttcacc ttcttcttcc gcgtacagca
    20881 gtggacccaa tttctcagag aagccccact gggtcaccat gttacgtgcc agattggtcg
    20941 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccatag atgatctctt
    21001 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
    21061 tgattgcgtc gccttcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
    21121 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
    21181 cttcgtggta agccgttgat tctttctgcg cttccgtcat caccatggag cgacgttccg
    21241 cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
    21301 tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca gcaccggaga
    21361 aaccaggagt accacgggca atgattgccg cgtcgatatc gggggccaat ggtacgcgac
    21421 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagccg accacaacct
    21481 gacggtcgaa acggccagga cgcagcaggg ccgggtcgag aacgtccgga cggttagtcg
    21541 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
    21601 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
    21661 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
    21721 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
    21781 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcaac gttttaccgg
    21841 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
    21901 ggctcggctc gcgcagatac tctaccagtt cagcaacttc ttcttttgct tcgtcgcagc
    21961 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
    22021 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
    22081 agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
    22141 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
    22201 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
    22261 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
    22321 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
    22381 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
    22441 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
    22501 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
    22561 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
    22621 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
    22681 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
    22741 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
    22801 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
    22861 cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg caagcgatga
    22921 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
    22981 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
    23041 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
    23101 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
    23161 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
    23221 atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg
    23281 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
    23341 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
    23401 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aactaaaacc
    23461 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
    23521 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
    23581 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
    23641 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
    23701 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
    23761 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
    23821 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
    23881 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
    23941 ccgaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg cgcgttcgac
    24001 agcttggctt cgatgtcttt aatacggcct tcgcagaaac cctgctgttc acgagctgcg
    24061 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    24121 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    24181 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    24241 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    24301 aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    24361 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    24421 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    24481 ttgaccagct gtatagcgtt cagtgttcag gccgcgaatg ttgatgagta cgttactcaa
    24541 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    24601 attgattacc acagtcagca gatggcgctg cccgccagta cgcagaaagt gattactgcg
    24661 ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct tgaaaccaaa
    24721 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    24781 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    24841 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    24901 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc tgccatagtt
    24961 gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga tatggctttt
    25021 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccga
    25081 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    25141 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    25201 gatggagcca gctatgccgg ggcaattctg aaagatgagt taaaacaggc aggtatcacc
    25261 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    25321 aaacagtcgg ccccgttgca cgatctgctt aagattatgc tgaaaaagtc ggacaacatg
    25381 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc tggaacatgg
    25441 cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    25501 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    25561 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    25621 ctgccgcttg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    25681 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    25741 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc tggctatgca
    25801 gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    25861 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggttt
    25921 tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt cccagtcatc
    25981 atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat gataatcatc
    26041 ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga ccgggttttc
    26101 aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac tcgccgcaga
    26161 gatcagataa tatttatctt cccagcccag cgcttcagca atcgctttcg ctttctcttc
    26221 agcttctgcc ttatccagca gatcgatctt attgaacact aaccaacgcg gtttcgccgc
    26281 cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt caaccggatc
    26341 ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac gttccaggtg
    26401 cttcaggaag cggataccca gacctgcgcc ttccgcagcg ccttcaatca gtcctggaat
    26461 atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac ttggcaccag
    26521 agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcgc gaataaaggt
    26581 cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca tcagctccag
    26641 cagcagctca cgcttatcac ccggcgtacc gttggttttc tgccgtggtg tacggttaac
    26701 ggacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa ccagcagacg
    26761 ctgaccgtgt ttagtcatat cgcccatggt ttcaccagta ccttggtcga ttatacgcgt
    26821 acctaccggc actttaatcg tcacgtcttt accgcgctta ccggtacaat cgcggcttgc
    26881 gccgttctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga taagcgtgtt
    26941 caggttttcg tcggcttcca tccatacgtc accaccatca ccaccgtcgc cgccatccgg
    27001 gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac cgccatcacc
    27061 tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg taaatcattc
    27121 gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc aaatacgatg
    27181 accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat aacctaaaag
    27241 gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat ctgaaaaaaa
    27301 cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc
    27361 catcgcttcc gccagggcgc catatcctac caatgtattc agtccgcaaa aaattaaaca
    27421 tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag ggaagagcgc
    27481 aattgtacat aaagcgtaca gtaaaaacag gatctgcggt gaggccagcc gacgcaataa
    27541 aaccttttgc gccacgccat aactcaccca aaccgtcgcc gcaccgaccc caaagataac
    27601 tccccaggtg taatcggtga gctttgtaaa tatctcgacc agactggtgt taaaaaacat
    27661 caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca ttttctcttt
    27721 caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa tcacctgcga
    27781 agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc caaacagccc
    27841 ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg
    27901 caacctcttc ttcaccgcaa gaatggcacc aaggccaata ctcgccatca agaaacggta
    27961 aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcga ttggcaacgc
    28021 cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct gctgcttcat
    28081 accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca tttttaccgg
    28141 ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg
    28201 aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt taacttcgaa
    28261 tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac ctacgttagc
    28321 gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca gaacggattc
    28381 gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt tacgtgtgga
    28441 gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc gctgatgcca
    28501 gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata gtgtttacga
    28561 cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac ttcagctttg
    28621 attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt tgcgatcatc
    28681 agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc caggcgaacg
    28741 gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc gtacatataa
    28801 aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat attcacaata
    28861 gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga
    28921 aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta caatcactct
    28981 atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc ggggcgaaaa
    29041 gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa gatatggcgg
    29101 gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccagctgatc aatcagttag
    29161 gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct gtactggctg
    29221 cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg atcgagttta
    29281 tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg cgcaggggta
    29341 aagctaccgc caacgctgca tttggcaatg ccgccagcgt gctggtaggc gattttattt
    29401 atacccgcgc gttccagatg atgaccagcc tcggttcgct caaagtgctg gaagtcatgt
    29461 cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaac gttaacgatc
    29521 cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg cgtctgtttg
    29581 aggctgccgc gcagtgttcc gggattctgg ctggctgtac gccggaagag gagaaaggcc
    29641 tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat ttactcgatt
    29701 acaatgccga tggcgaacag ttaggtaaaa atgtcggcga cgatctgaac gaaggtaaac
    29761 cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca cagatgatcc
    29821 gtaccgccat cgaacagggt aacggtcgcc atcttctgga accggttctg gaagcaatga
    29881 acgcttgtgg atctcttgaa tggacgcgtc agcgtgccga ggaagaagca gacaaagcca
    29941 tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc ggcctcgcgc
    30001 acatcgctgt tcaacgcgat cgttaatccc ctcccctcat cccgcgcagc gcgcggggtg
    30061 gttccagtaa attccataaa aacacttact cagctctata acacctgaaa aacaggctgt
    30121 gcatctttat atattctgaa tattcacact ccttacagga actttttaga gcaatcggcc
    30181 atcaggagta tagtgatgct cgacagaaga agtgttctga atgaaagcga ctaacttaag
    30241 gagtgaggaa agatggaaag taatttcatt gactggcatc ccgctgacat cattgcgggt
    30301 ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca gaaatggttt gagttcctca
    30361 acgctggcga atgcattatc gcgcccatgg ccgaaaggag agatgattat tgcgaaagcc
    30421 ctgggaactg acccctgggt tatctggcca tcacgctacc atgatccgca gacccatgag
    30481 tttatcgaca gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg agtggtctgg
    30541 cggtagcccc gcgaacgggg ctgccagctc tcagacgatt actcgccttt cacacgctca
    30601 atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg atcgatgtga
    30661 taaatacgat caaccaccgt cgtcccttcc gcaatacagc cagccagcac caggcttgct
    30721 gatgcacgca gatcggttgc cataacctgt gcgccagaaa gtttttcaac accgtgacaa
    30781 ataacggtat tgctttcgat ttcggcatgc gcgcccatac ggctaagctc cggcacatgc
    30841 ataaagcggt tttcaaagac cgtttcggtg atgaatccgg tcccttctgc caccaggttc
    30901 aacagcgtga actgggcctg catatcggtc gggaatgccg gatgcggcgc ggtacgtacg
    30961 ttaacagcct tcggacgttt gccatgcata tccaggctaa tccagtcttc gccgacttcg
    31021 atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc tggctgcgcg
    31081 ttacggcaga taattttgcc gcgagaaatc gccgccgcca ccaggaaagt accggtttcg
    31141 atacgatccg gtagcacgcg atagacaccg ccgcctaaac gttccacacc ttcgatgacg
    31201 atacgatcgg tgccctgacc gctaattttc gcacccagcg taatcaggaa gttcgcggta
    31261 tcgacgattt ccggttcacg cgctgcgttt tcaataatcg tggtaccttc cgccagggtt
    31321 gcagcacaca tgatggtcac cgttgcgcca acgctgactt tatccatcac gatatgtgcg
    31381 cctttcagac gaccatcgac ggaagcttta acgtaacctt cttccagttt gatggtcgcg
    31441 cctaattgtt cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat cgtacaaccg
    31501 ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca gcggccccag tgcccagata
    31561 gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga atacattaac gtcgcgggca
    31621 tcaatatgca cagaaccatt acgttctact ttcgcaccca gctggcttag cagcttcatt
    31681 gatgtatcga cgtctttcag tttcgggacg ttctggatct ctaccggttc ttccgccagt
    31741 agagcggcaa aaaggatagg cagagcagca tttttagcgc cggaaattgt gacttcgccc
    31801 tggagcttcg ttggcccctg aacacgaaat ttatccattt agtttgttct cagttaacaa
    31861 ttcatatccg ctaccggcga atcgcccata gctcaaaagc cgttcagttt gcgatcgcgc
    31921 gcccactccg caggggtata cgctttgatc gacacagcat gaatgcggtt atccgcaata
    31981 tattccatca gcgggccata gaccgtctgc tgttttttaa cccgactcat gccgtcaaac
    32041 aactcaccca cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg gacttcctgg
    32101 agggagagag cgttcatcaa cacgctctga atttcattat tttccatggg atcttcaatc
    32161 atcagttaat aaaccagcga aacatcttag agcaaagttg cgctggcata aataagcaaa
    32221 aagcctcgct gataaatcag acaaggctcg acttgcaggc aggtttgccg gacaggcggt
    32281 taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac
    32341 aatttcgcga gggtatacac tttgtcgttt accccctgaa gcgtcacgtt gttgccctgc
    32401 tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac
    32461 acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc
    32521 ggtagcagaa cgtcctgatc cagctctccg gataacgcca gcgtgtcacc cgtctgcatc
    32581 cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg
    32641 atttcagttg cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt
    32701 tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagtttc
    32761 ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcggacgg ccattcgggt
    32821 caataatggt aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt tctggcgcaa
    32881 tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact
    32941 cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg taatactggc
    33001 ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcaacaa
    33061 tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga
    33121 acgttttctg cgccgcctcg tccatcagct tatacggatt ggtctggtct gccgcggttg
    33181 ccgcactcag aggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc
    33241 ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg
    33301 cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact
    33361 gaccaatgag atcttccagc accatcgcag acttagtgtc ctgaattgta tcgccatcct
    33421 tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt
    33481 cccccagcag gccggaagta cgaatgctca gcgaactggt atctggaatg tggttataac
    33541 gttgttcaat ttccagcgtt acgcgtggca gataggtttt cgggtccagc gtaatatccg
    33601 ccacccggcc cacaacaacg ccaccaatac tgaccggaga gcgcgctttc aggccgccaa
    33661 tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg
    33721 ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc taataaaaag atacccaccc
    33781 aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca
    33841 aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta
    33901 atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg
    33961 atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca
    34021 ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga
    34081 gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc
    34141 ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat catctccata
    34201 ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca
    34261 cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc
    34321 accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc
    34381 cccaacacca ttccgatgaa cacgccagaa accacaataa tcagcatcga caggacgccg
    34441 acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc
    34501 agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta
    34561 tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc
    34621 agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag
    34681 aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac
    34741 aatttttttg tccgccagga tccaggcgtg atccgcaata cttaacactt ccggcacatc
    34801 gtgagaaacc accacacaag tcacgcccag cgcgctgttc agctcagaaa tcagcttcac
    34861 cagtacgccc atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca tgatgagatc
    34921 cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cagaaagttc
    34981 agaaggcatt agcttagccg ctccacgcag ccccacggcc tccagcttca tcatcactgt
    35041 actatgcaac aatggcgcgg gaagttgggt atgttcgcgc agtggatagg cgacgttgtc
    35101 aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg
    35161 cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaaggatctc
    35221 accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc
    35281 cgatggcccc atgattgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa
    35341 gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga
    35401 ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac
    35461 cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt
    35521 tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt
    35581 cagcggaccg gcgagtatac ctgaagaaag gatgttagat gcttttagct acggcactgt
    35641 taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta
    35701 ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg
    35761 gtacatcgtt accagaagtc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt
    35821 tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg
    35881 ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa
    35941 tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt agtcgcagcg
    36001 atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac
    36061 gtcaggctga acgtcagggg actgacagcc tgaccagaga gcagcttgca gagctgccgc
    36121 gtgacggcgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag
    36181 tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg
    36241 agttgacgat gggtctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg
    36301 caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc attggcgcaa
    36361 acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg
    36421 atccactggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt
    36481 tgctgtgctg gcggcgctcc ccacaaccgg gccgtggtgt aggggtatta ttaactggcg
    36541 gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg
    36601 aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa
    36661 gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat
    36721 ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg
    36781 gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac cggtacacct
    36841 tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag
    36901 gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca
    36961 gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg
    37021 gcgcgcgctg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg
    37081 ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg
    37141 ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacaccc aggcggcgca
    37201 ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg
    37261 catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt
    37321 ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat
    37381 ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtattgc cgatgtgatg
    37441 acgccggggg gaatacgtgt gcgccctggc attctggccg ttgaggcact gaacttaatg
    37501 cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta
    37561 catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga
    37621 gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatggcaa
    37681 aagcagagaa cattcgtctg ctgatcctcg acgttgatgg cgtactgtca gatggcctga
    37741 tttatatggg caataacggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca
    37801 ttcgttgtgt gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac
    37861 tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca
    37921 aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa aatgtggctt
    37981 atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg
    38041 tggccgatgc gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc attgctggcg
    38101 gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct ggcgcagggc aaactggatg
    38161 aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact
    38221 ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt
    38281 cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc
    38341 agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc
    38401 cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg
    38461 gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca
    38521 cgttgaagtc aacgcactcg tgccggactc tcaacttcgc agaatcacga cggataacgc
    38581 gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac
    38641 aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct
    38701 gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg
    38761 tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg
    38821 atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta
    38881 cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg
    38941 ttgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg
    39001 caacgttcta ccagatgcag gacaacggta aaccggttga aggtcacgct tcccagatgc
    39061 actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggttg
    39121 atagcaacat taagggcgat aagatcactt acctggtaaa agagcagaaa atgcaggctt
    39181 tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggacaaaa
    39241 acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa
    39301 ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga
    39361 ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca
    39421 ctttctatat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg
    39481 acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac
    39541 aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa
    39601 ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt
    39661 ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc
    39721 gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt
    39781 ttgccggtgt tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg
    39841 acagcggcct gggcgtgctg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg
    39901 aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct
    39961 tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta
    40021 gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact
    40081 caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca
    40141 gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct
    40201 tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacgct
    40261 ggacaccgcc gatgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag
    40321 ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga
    40381 tgagctgccg gtctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca
    40441 ggtcgagctg acaccgtttt ccgacactga ccgcgctatt gctacctcta tcgtcgatgc
    40501 cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga
    40561 tgaagagatc gacatcgacg aggttgaagc cgtccttaag cggatccaac ggtttgatcc
    40621 ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta atccaactct cccaattcga
    40681 taaaaccacg ccgtggctgg aagaggccag attgatcatt agcgatcatc tcgatctgtt
    40741 agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa
    40801 agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagactgg
    40861 cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacagt
    40921 agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg
    40981 caataacgct cgcaatgatg gtgacagcca gtttatccgc agcaatctgc aggatgccaa
    41041 atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat
    41101 cgttgaacag cagcaagcct tctttgagca aggcgaagaa tatatgaaac cgatggtact
    41161 ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca
    41221 aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt
    41281 caataccgag ggcggcggcg aagcttcctc cacggcgatt cgtgcgctgg tgaagaaatt
    41341 aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttgctgtc
    41401 ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat
    41461 tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc
    41521 agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag
    41581 ccaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga
    41641 aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc
    41701 atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg
    41761 cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag
    41821 catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag
    41881 cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa
    41941 ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga
    42001 gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt agcctgccgc
    42061 ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca
    42121 atggtattgc cattccgcat ggcaaactgg aagaagatac tctgcgcgcc gttggcgttt
    42181 tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggacttac
    42241 tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcgctgg
    42301 tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg
    42361 aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg
    42421 gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg
    42481 gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact
    42541 gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaactctg gccgatcgcg
    42601 agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg
    42661 aacaggcgat gagtaacctg cctgacgctt tctcaccgca actactgttc ctggatgccg
    42721 accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca
    42781 aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt
    42841 cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg gctgaaatgc
    42901 tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac catggtcttt gagtctttcg
    42961 gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga
    43021 acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataaacct gtcgccgcgt
    43081 tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgaac
    43141 tatggttacc tatgctggaa accaacaacc gtagctactt gacggtcgcc attggttgta
    43201 ccggcggaaa gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc
    43261 gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa
    43321 gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt
    43381 tgaattaatg cagggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc
    43441 cgaagccaac agcgttattg cgctgctgat gttggattct gccaaaggac ggcagattga
    43501 agttgaagcg accggcccac aggaagagga agcactggcc gccgttatcg ccctctttaa
    43561 ctctggtttt gatgaagatt aatcttcatc actttgacat acaactccct tcaaactccc
    43621 cccgacaata agaaaatcac gtactgaaat cgttctcaat caacgtcatt tgtacatttt
    43681 gtgcgctttt cactttcaga agaaccttaa gaaaacctta agagacattg tttaggtttt
    43741 gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag atggcatgat
    43801 tcgcttatct gaacaaagtc ccctgggcac cggaagacat cggaaatgtt atgcgcatcc
    43861 ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga
    43921 gatccgccgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag actggagtgg
    43981 aataccgcgc tatcacggta ccgttgagac ggattgcgga accggttatg tctacgatgt
    44041 gattgcagat tttgacggca aaccctccat tacgctgacc gaatttgctg aacaatgccg
    44101 ttacgaagaa gacattgcac aactgcgcca gctactgaaa cagctgaagc gttatttgca
    44161 ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc accgcatcag
    44221 tgagtcagag gttatcccgg tcgtctgtga caacattggt gaaagcacat tgattccact
    44281 ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag cggttatgga aacgatttat
    44341 cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg agagcaaaac
    44401 gctggccctc acttcgcgcg aagcttaatc cagctggtgt tgttgcataa acggctcacc
    44461 gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg
    44521 cgaggagact ttgaaacgaa gtggattagg taatacagct gccagtaatg cagcttccga
    44581 ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa
    44641 cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct ttttgctcca
    44701 gactgtttct atccccagcg ttaatccagc ttccagccct tttcgtaccc agctacgccc
    44761 atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac cgcgaatacg
    44821 gttttcattg cgctcgttgt gcgccagggc tttctcaatg gaagcgacat caaagcccca
    44881 gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc acggcgagat
    44941 ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattgccat gcagccaggc
    45001 gctgacctgt cgctcgacca ttaccgctga gaagggaaca ggcgcaacac taaacaacgc
    45061 gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa aacggcgaac
    45121 gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac gcgggaaacc
    45181 agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata tgctggggtg
    45241 gtgacaatct tattgtcttc atcaaccacg atatcatcga caggacaggg cacatgctcc
    45301 gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt caaacgcagc
    45361 gggaaatcga aaattttcgg cagcatcgcc ggggcaatac acataaaacc aagcggtttt
    45421 ccggcctgat gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt gcattcgcta
    45481 ccaagagtgg caaaattgct taaattcttc gccgcgccaa acccccccgg cacaatcaac
    45541 gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg cgttattcgt
    45601 gccgcttcaa tcagcacgtt gcgcgtttcc gtcatcgctt cgccagttaa atggttgata
    45661 acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata
    45721 gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc gcatccgctc
    45781 agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga aacacattaa
    45841 tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac aaatcattta
    45901 agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc
    45961 ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc gcgcaccccg
    46021 gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca cttctacgtc
    46081 gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt cccaggcagg
    46141 aaggtccgga gactgaattt gctgacccag ctgttgcaga tggcgtaacc ccactgaccc
    46201 cgccgcacct ttaattttat gtccttcctc tacaatgcct tttttatcct gcgccgtcag
    46261 attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg ctaacccgtc
    46321 ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa tatcgagcaa
    46381 tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat cctgggtatc
    46441 ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa agcggcttac tcagcacatc
    46501 atccattcca gcattgaggt actcttgttt gtctttcagc acgttagcgg ttaaggccac
    46561 cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga tatccagccc
    46621 ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg gtttaaacat
    46681 ctccagcgcc gccttgccgg tcatggcgac atcaacgctg ttacctaatt tttccagcac
    46741 agaacgcgca acaatcacgt tcagttcaat gtcttccacc agcagcacat tcagcgccgg
    46801 taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg acggtgcgtg
    46861 gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa tatcgccgcc
    46921 catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg tggcaggttt
    46981 accgccatga ctgtctttca cctggtaata catggcgaaa attttatcca gctcatcctg
    47041 cggaatgccg ataccagagt cttccacttc aaaatgcagc atatcgcctt catcgtagcg
    47101 cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga tgaggttcca
    47161 caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg gcagcgtcgg
    47221 ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat gcggagagat tttccagatc
    47281 ggcaaggaag ctggtgaaat caaccggttg attatcaagc tggaccttgc gccgttccat
    47341 cttatccatg tcgataatat cgttaaagat attccccagc gtgacggccg aaacatggat
    47401 ggtcttgaga tatttttcct gctcggcggt gagttcggta tccagcagaa tacggctcag
    47461 gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga taaacgtcgt
    47521 tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg
    47581 accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca ctttacggat
    47641 ttcaaagcag gctttgcgcc cgtccgggta atccagccac tgttcatagg tcagtgacac
    47701 attatgacgg aacacttttt catcggtttc aatgactttt gcggcggctt ccggtgagta
    47761 aacatcagca ggtttcaggt gaaccagttg tttttcgctt tttccggtca gcagctccat
    47821 cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca ggtcgggtga
    47881 agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt gtgtctcttc
    47941 gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg cttccgcttt
    48001 ttcacgaacg gcgatttcct gatttagctg ggcaatatta tcttttaact gaacgttgag
    48061 gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca gacgttgtcg
    48121 tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg gcgtaatcag
    48181 caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat gcagcaccat
    48241 ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca gcaacattga
    48301 gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca gcagacgaat
    48361 ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa ttctgacagt
    48421 tacgttataa attgtgtgag aaatgcggaa ttcccctcaa cgacctactc cgttggaggt
    48481 atccacggac gttccagcgc cgatccctgt acgccatgtt cattcagata gcgatccagc
    48541 tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt cgggcgacgg
    48601 gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat catttctccg
    48661 gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc ccaggctttc
    48721 gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc cacgccggtt
    48781 tcaaccactc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg caggccgaca
    48841 attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt acgctgataa
    48901 caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc ggtttgtagc
    48961 cccagctcca gccacacttc gtaaccctgg tccttatatt cgcaaagcag atccagcacc
    49021 gcatccggca cgcagtccgg gcgggtacca acacacaaac cgacaatatt ggcctggctc
    49081 accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct ggtatacgcc
    49141 tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg cgccagttgc
    49201 tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg
    49261 cagccaccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc gtgcagcgtc
    49321 agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat attgactaat
    49381 ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc ctgccatttt
    49441 tagctcaatt cggcgatgac ctggatcaat cgtcccggcc tgcttttatc aactgcataa
    49501 tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc ttatcttgtg
    49561 tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa acgtcattac
    49621 caattaaggc agtataaaat gctggctttc tcgtcagttc aaggcaggat aagggttaac
    49681 acgcctttat gacagtcagg aattgactgt ttctctaacg acttcccttt tagccttaaa
    49741 gataaaatct attttaattt cagtcattta ataaagaatt ttgcgctaaa gcacatttct
    49801 gtaccaataa gcttgccatt tgacctgtat cagctttccc gataagttgg aaatccgctg
    49861 gaagctttct ggatgagcag cctgctcatc atatttatgc agtaattgag atcccctctt
    49921 caccgtatta accgatgcga aaaggacaac aagggggcga atgcgaggcg cgcgtatgac
    49981 acgcaaaccc cgtcgccacg ctctttctgt gcccgtgcgc agcggttcgg aagtggggtt
    50041 cccgcagagc ctgggggagg ttcacgatat gttgtacgat aaatcccttg agagggataa
    50101 ctgtggtttc ggcctgatcg cccacataga aggcgaacct agccacaagg tagtgcgtac
    50161 tgcaatacac gcactggccc gcatgcagca ccgtggcgcg attctcgccg atggtaaaac
    50221 cggcgacggt tgcggcttgc tgttacaaaa accggatcgc ttttttcgca tcgttgcgca
    50281 ggagcgcggc tggcgtttag caaaaaacta cgccgtcggg atgctattcc tgaataaaga
    50341 tcctgaactc gccgctgccg cacgccgcat cgttgaagaa gaactgcaac gcgaaacctt
    50401 gtcgattgtg ggctggcgtg atgtccccac taacgaaggc gtgctgggtg aaatcgccct
    50461 ctcctctctg ccacgcattg agcaaatttt tgtgaacgcc ccggcaggct ggcgtccgcg
    50521 cgatatggag cgccgtctgt ttatcgcccg ccgccgcatt gaaaagcgtc tcgaagccga
    50581 caaagacttc tacgtctgta gcctgtcgaa tctggtgaac atctataaag gtctgtgtat
    50641 gccggcggat ctgccgcgct tttatctgga tcttgcggac ctgcgtctgg aatcggccat
    50701 ctgcctgttc caccagcgct tctccactaa caccgtaccg cgctggccgc tggcgcagcc
    50761 gttccgctac ctggcgcata acggtgaaat caacaccatc accggtaacc gccaatgggc
    50821 gcgtgcgcgt acctataaat tccagacgcc gcttatccct gatctgcacg acgccgcacc
    50881 gttcgtcaac gaaaccggct ctgactccag ttcgatggat aacatgctgg aactgctgct
    50941 ggcaggcggg atggatatca tccgcgccat gcgtctatta gtaccacccg cctggcagaa
    51001 caacccggat atggacccgg aactgcgtgc cttctttgac tttaactcca tgcatatgga
    51061 gccgtgggat ggcccggcgg gcatcgtgat gtccgatggt cgcttcgctg cctgtaacct
    51121 cgaccgtaac ggtctgcgtc cggcacgcta cgtcattacc aaagacaagc tcatcacctg
    51181 cgcctctgaa gtcggtatct gggattatca gcctgacgaa gtggtcgaaa aaggccgcgt
    51241 cgggccaggc gaactgatgg tgatcgacac ccgcagtggg cgtattctgc actcggcaga
    51301 aaccgatgac gatctgaaaa gccgccatcc atataaagag tggatggaga aaaacgtccg
    51361 ccgactggta ccgtttgaag atctgcccga tgaagaagtg ggtagccgcg aactggacga
    51421 cgacacgctt gccagctacc agaaacagtt taactacagc gcggaagagc tggactccgt
    51481 aattcgcgta ctgggcgaaa acggtcagga agcggtcggt tcgatgggcg atgatacccc
    51541 attcgccgtg ctctccagtc agccgcgcat tatttacgac tacttccgcc agcagtttgc
    51601 ccaggtgact aacccgccaa tcgacccgct gcgtgaagcg catgttatgt cgctcgccac
    51661 cagtatcggt cgtgaaatga acgtcttttg cgaagcagag ggccaggcgc accgtttaag
    51721 ctttaaatcg ccgattctgc tctactccga tttcaaacag ctcacgacga tgaaagagga
    51781 gcactaccgc gcagatacgc tggatatcac ctttgacgtc actaaaacca cgctcgaagc
    51841 gacagtcaaa gagctgtgcg acaaagccga aaaaatggta cgtagcggca ccgtgctgct
    51901 ggtgctctcc gaccggaata tcgctaaaga tcgcctgccg gttccagccc cgatggcggt
    51961 tggcgcgatc cagacccgtc tggtcgatca aagcctgcgt tgcgatgcca acatcatcgt
    52021 cgaaaccgcc agcgcccgcg atccgcacca cttcgccgtg ttgctgggct tcggcgcgac
    52081 ggctatttat ccataccttg cctatgaaac gctgggccgc ctggtagaca cccatgcgat
    52141 tgccaaagat tatcgtaccg tgatgctcaa ctaccgtaac ggcatcaaca aaggcttgta
    52201 caaaatcatg tccaaaatgg gcatctccac catcgcctct taccgctgct cgaaactgtt
    52261 tgaagcggtc ggtctgcacg atgatgtagt gggcctgtgc ttccaggggg cggtcagccg
    52321 cattggtggg gcgagctttg aagacttcca gcaggatctg ctgaacctgt cgaaacgtgc
    52381 ctggctggcg cgtaagccca tcagccaggg cggcctgctg aaatacgtcc acggcggcga
    52441 ataccacgct tacaacccgg acgtggtgcg cacgctgcaa caggcggtac aaagcggcga
    52501 gtacagcgac tatcaggaat acgcgaagct ggttaatgag cgtccggcaa ccacgctgcg
    52561 cgatctgctg gcaattacgc cgggtgaaaa cgcggtcaac attgctgatg ttgaaccggc
    52621 aagcgaactg tttaaacgct ttgataccgc cgcgatgtct atcggcgcgt taagcccgga
    52681 agcccacgag gcgctggcgg aagcgatgaa tagcatcggc ggtaactcga actccggtga
    52741 aggcggcgaa gacccggcgc gctatggcac taacaaagtg tcgcgcatca agcaggtggc
    52801 ttctggtcgc ttcggcgtta ctccggcgta tctggtcaat gccgacgtca ttcagattaa
    52861 agtcgcccag ggcgcgaagc cgggcgaagg cggtcagttg ccgggcgaca aagtcactcc
    52921 ttacatcgcc aaactgcgct attcggtgcc cggagtgacg ctgatctccc cgccgccgca
    52981 ccacgatatc tactctatcg aggacttagc gcagctcatt ttcgacctca agcaggttaa
    53041 cccgaaagcg atgatctccg tgaagctggt ttccgaaccg ggcgtaggca ccatcgcgac
    53101 tggcgtggca aaagcttatg ccgatttaat caccatcgca ggctatgacg gtggcaccgg
    53161 cgcaagcccg ctgtcgtcag tgaaatacgc aggttgcccg tgggagctgg ggcttgttga
    53221 aacccagcag gcgctggttg ccaatggtct gcgtcataag atccgtttgc aggtcgatgg
    53281 cggcctgaaa accggtgttg atatcatcaa agcggcgatt ctcggcgcag aaagcttcgg
    53341 cttcggcact ggcccgatgg tggcgctcgg ctgtaaatat ctacgtattt gccatctgaa
    53401 caactgcgca acgggtgtag caactcagga tgacaaactg cgtaagaacc actatcacgg
    53461 cctgccgttc aaggttacca attactttga atttattgcc cgtgaaaccc gcgagctgat
    53521 ggcacagctg ggcgtaacgc gtctggtgga tctgattggt cgcaccgacc tgctgaaaga
    53581 actggacggt ttcaccgcca aacagcaaaa actggcgctg tcgaagctgc tggagaccgc
    53641 cgaaccgcat ccaggtaagg cactctactg caccgaaaac aacccaccgt ttgataacgg
    53701 cctgctgaac gcgcagttgc tgcaacaggc gaaaccgttt gtcgatgaac gccagagcaa
    53761 aaccttctgg ttcgatattc gcaacaccga ccgttctgtc ggcgcgtcgc tttcaggcta
    53821 tatcgcccag acgcacggcg atcaggggct ggcagccgat cctatcaaag cgtacttcaa
    53881 cggcaccgca ggccagagtt tcggtgtgtg gaacgcgggc ggcgtggaac tgtacctgac
    53941 cggggatgcc aacgactatg tcggtaaagg catggcgggc ggcttaatcg ccattcgtcc
    54001 tccggttggt tccgccttcc gcagccatga agcaagtatt atcggcaaca cctgcctgta
    54061 tggcgcgacc ggtggtcgtc tgtatgccgc aggccgcgcg ggtgaacgtt tcggcgtgcg
    54121 gaactccggt gctatcaccg tggtagaagg cattggcgac aacggctgtg aatatatgac
    54181 gggcggtatt gtctgtattc tgggtaaaac cggcgttaac ttcggtgcgg gcatgaccgg
    54241 cggcttcgct tacgttctcg atgaaagcgg tgatttccgc aaacgcgtta acccggaact
    54301 ggtcgaggtc ttaagcgttg acgatctggc gatccatgaa gagcatctgc gcggtcttat
    54361 caccgagcat gttcagcata ctggctctca gcgcggtgaa gagattctgg cgaactggtc
    54421 aaccttcgcc actaaatttg cgctggttaa accgaagtcc agtgatgtaa aagcactgct
    54481 gggtcatcgt agtcgtagcg cagcagagtt gcgcgtgcag gcgcagtaag gggtagcaac
    54541 aatgagtcag aatgtttatc aatttatcga cctgcagcgc gttgatccgc caaagaaacc
    54601 gctgaagatc cgcaaaattg agtttgttga aatttacgag ccgttttccg aaggccaggc
    54661 caaagcgcag gctgaccgct gcctgtcgtg cggcaaccca tactgcgagt ggaaatgtcc
    54721 ggtacacaac tacatcccga actggctgaa gctcgccaac gaggggcgta tttttgaagc
    54781 ggcggaactg tcgcaccaga ccaaccccct gccggaagtt tgcggacgag tctgcccgca
    54841 agaccgtctg tgcgaaggtt cctgcactct gaacgatgag tttggcgcgg tgaccatcgg
    54901 caacattgag cgttatatca atgataaagc gttcgaaatg ggctggcgtc cggatatgtc
    54961 cggcgtgaag caaaccggta aaaaagtggc gattatcggc gcaggcccgg caggtctggc
    55021 gtgtgcggat gtcctgacgc gtaacggcgt aaaagccgtt gtcttcgacc gtcatccaga
    55081 aattggcggg ctgctgacct tcggtattcc ggccttcaag ctggaaaaag aggtaatgac
    55141 gcgtcgccgt gaaatcttca ccggcatggg tattgaattc aaactcaata ccgaagtggg
    55201 ccgcgacgtg cagctggacg atctgctgag tgattacgat gccgtcttcc ttggcgtcgg
    55261 gacttatcag tcaatgcgcg gcgggctgga aaacgaagac gccgatggcg tgtacgcagc
    55321 gctgccgttc cttatcgcca acaccaaaca gttaatgggc tttggtgaaa cccgcgacga
    55381 accgttcgtc agcatggaag gcaaacgcgt ggtggtcctt ggcggtggcg acactgcgat
    55441 ggactgcgtg cgtacgtccg tgcgccaggg agcgaagcac gttacctgtg cctatcgtcg
    55501 tgatgaagag aacatgccgg gttcccgccg cgaagtgaaa aacgcgcggg aagaaggcgt
    55561 agagttcaaa ttcaacgtcc agccactggg gattgaagtg aacggtaacg gtaaagtcag
    55621 cggcgtaaaa atggtacgta ccgaaatggg cgaaccggat gccaaaggcc gtcgccgcgc
    55681 ggagatcgtt gcaggttccg aacatatcgt tccggcagat gcagtgatca tggcgtttgg
    55741 tttccgtcca cacaacatgg aatggctggc aaaacacagc gtcgagctgg attcacaagg
    55801 ccgcatcatc gccccggaag gcagcgacaa cgccttccag accagcaacc cgaaaatctt
    55861 tgctggcggc gatatcgtcc gtggttccga tctggtggta accgctattg ccgaaggtcg
    55921 taaggcggca gacggtatta tgaactggct ggaagtttaa gctctgtccc agcgccgcct
    55981 cctcagaaaa gagtcggcgc tgaaattctc ctttctttct ggaaagcgcc tcgcataatc
    56041 tgaagaaaac caatgtaata cgctcatctg gattgttacc ggtagatgcc cggcgtatag
    56101 ctgattccat tattcaacgc agcgccatat aaagggacag ataatggaat cgctctcaga
    56161 gagaacctca acaggctacc agcaaatcca cgacggtatt attcatctgg tcgatagcgc
    56221 ccggacggaa acggtacgta gcgttaacgc gctgatgacc gcgacatact gggaaattgg
    56281 ccgacgaatt gtcgaatttg aacaaggtgg cgaggccagg gctgcgtatg gtgcgcagct
    56341 aatcaagcga ctatcaaagg atttaagtct aaggtataag cgtgggttct ctgcaaaaaa
    56401 cttacgccaa atgaggcttt tttacctctt ttttcaacat gttgaaattc gccagacagt
    56461 gtctggcgaa ttaacacctg ccctggtcca cctacgtccg tttactttcc gttaaaaacg
    56521 ctgacgcccg cagcttttat gaaaaagaga cgctccgctg tggctggtct gttcgccagc
    56581 tagaacggca aattgcgacc caattttatg agcgaacact actgtcacat gacaaatcag
    56641 ccatgctgca acaacacgct cctaccgaga cgcatattct tccgcaacag gcgatacgcg
    56701 atccctttgt gctcgaattt ctggaattga aagatgaata ttcagaatct gatttcgagg
    56761 aggcgctgat caaccacctg atggatttca tgctggaact tggggatgat tttgcctttg
    56821 ttggtcggca gcgaaggtta cgcattgatg acaactggtt tcgtgtcgat ctgctgtttt
    56881 tccaccgccg tttacgctgc ctactaatcg tcgatctaaa agtgggcaaa ttcagctata
    56941 gcgatgccgg acagatgaat atgtatctca actacgccaa agagcactgg acgctaccgg
    57001 atgaaaatcc gcccatcggt ctggttctct gtgcagagaa aggagccgga gaagcacatt
    57061 atgctctggc aggtttgcct aacaccgttc tggcaagcga atataagatg caactacctg
    57121 atgagaaacg actcgcagat gaactcgttc gaacacaggc ggtgctagag gaaggctata
    57181 gacgccgtta atttcagcgg tttcgaattc tgcagacagt gtctgcagaa tctttaaaac
    57241 gaactaagtt ttaagccatt aaatcagcct taaccttcac cacaaccttt ttaatctctc
    57301 caggctcgcc gacaacgcat cctggtttat gcggttcacc tggcataaac acggcgaaca
    57361 ttcccggttt taagatgatg gcttgctcgt tatcaatggt gctgcaaagc tgataatcat
    57421 cctcatggtg gaactcttca cactggcgcg cagtgcctgc catgccaaac agaatccgtt
    57481 cctcaccgtt taataacagt tggatatcaa tgtattgctc gtgcaattcc gcttttttct
    57541 cgacgggcga ttgagtgtta aacgtcatga cattcataaa gatattgtcg ccctgtaatt
    57601 cgtaacgacc cggcgctttt tcttgcggtc tggcagctaa tgccagcgtt aacgcgtcct
    57661 gtaacgcagg atgtaaccca gcagacggta atgactgtac ttcacccatc atcataattt
    57721 ttctccctgg gccaacagcg cagccccaag taaacctgca tcgtggcggt agtgcgccgc
    57781 cagtaaatca acatgaaatg ccgctggctc ctgcgccaga tacgtttcca ccagcgccag
    57841 atacccttct gccagcccaa cgctgccacc gaccaccacg cactggcaat cagttgtggc
    57901 tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag cggtgaatca gctgctgcgc
    57961 ctgctcgtcg ccctgcccgg cgcgtgtgaa aatagttctc gcatccgcgc ccgccagttc
    58021 accctgcgct gccgccgcaa tgccgcgacc agaagcaatc gcctccacgc aacccgtgcg
    58081 tccacagcca cagaccgggc cgtgtggatc ggcaagcgta tgcccgatgt gccccgccag
    58141 accgccaggg ccggtaagca gtttgccgcc gctcactaca ccgccgccga cgccagtgga
    58201 aacggtgata aagaccatat cggttatatc gccttccagc gcctgatact ctgcccatgc
    58261 tgcggcctgc gcgtcgttaa tggcaatggt cggcaaattg gtaagttgtt ccagcgtttt
    58321 gactaacgga aagtgtagca atccaccgag attatgcgga ttcagcgcca gcaaactgcc
    58381 gtcgcgaata attccggttg aagcgatggc aacccgctgc gcatgagctt gcaacggaga
    58441 gactaatgcg gataaggcat cacgcaaggc ttctggtgtc tggctggctg gcgtaggaag
    58501 ttcacgacga tcgcggatct gcccgtcagc gccaatcagc gcggcggcaa gtttagtacc
    58561 gccgatatca atcgccagcg tggtcatagc accgcctttt tcatcgctgt gttgtaccac
    58621 tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg tcaccgccca cgcgccgtgg
    58681 cgcatcgcat ctgccgcctg agcaggcgtg ttgtaacgcc cttcggcaat caccctacat
    58741 ccggcgtcgc tcaacgtttt caccagcgcc agatccggct cttctggcgt ttcaggcgtg
    58801 gtatagccag aaagcgtagt gccaataatt tcggctccca gcttttggca tgccaggccg
    58861 tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt gatggtgaat acgtgccagc
    58921 agcgtttcaa caggcaccgg acgcgggcgg tcggtgccgt caatggcgat aatgtccgcg
    58981 cccgcctgcg ccagcgcatc aacatcttca atataggccg tgatgcgtac cggagaatcc
    59041 tccagatcgc gtttcacaat tccaataatc ggcacgctca ccaccgcacg cgtggcttgc
    59101 agatttgcca caccttcaat gcgaatggca accgcgcccg cctgttctgc cgctaatgcc
    59161 atggcggcga cgatttcggg tttatcgagc gggctgtccg gaaccggctg gcaggagaca
    59221 atcaggccac cgttagcagc gattttttga tccagttgtg caagtaacga catacatctt
    59281 cccttagcga aaggcccggt acatagaccg ggcaacagga ttaacttttg gttttgacta
    59341 aatcgttttt ggcgctgcca aatggcacgg caccgctaaa tggtttgccg tcgatagcgt
    59401 catgagtacg caatgcttcc gggcgcagcc aacgctgaac gcgggaaggc atatccagac
    59461 caatcagcag gatcaccaca aatgtcagac tgaacgatag cgatgccagc gcagtaccca
    59521 gatccagacg ttgagcgatc aacgcgccga tgattggggc cagtgcgccg cccaatgcac
    59581 caacgttgta ggtaaagccc aggcccgctg cacgctggtc ggtatcgaaa taaccgccaa
    59641 tcagtttcgg taagatcccg gcgatccctt gtccaagcat ttgctggaag aacagtaaca
    59701 gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac cggaataatc aacagctgcg
    59761 aggccaacag gctacaaacg tacgctttgc gggttcccag ccagtcaccg aggaagccgc
    59821 cgacacagca ccccaccgca gccccaaagc cgctaaagaa cagcacattg gcgacagtat
    59881 gcgggttata agccagatcg gttttcagat acgttggcag cagcgcctga atcggccatg
    59941 agtagaggaa agcaaacaac acgaccacca tcagcattac gcccgttggc cagcgtttac
    60001 ccgtactttg caccataaag ctgataaaga ttgcggcgca taacagccca agaacagcga
    60061 cgatcgcggc attttgcagg ttaccggcga agcaaaacca cagcgcagta gccgccgcca
    60121 gtgtcattac gatattggca atgcgatgtt caccacggta gagaatatct accatcgtgc
    60181 gtactggtgc tttacctgca tgtttctctt tccagtcttc cgcttccggg atgtttttac
    60241 gcagccagag agcaaagatg ataggcaaaa tgccgataaa gaacagcgca cgccagcccc
    60301 agaccggaac caccaggcta tagacctgag cggcaacgac ggcccccaca gagaagcctg
    60361 aaatcagaaa accactggct ttgttacgca gatgttttgg ccagctttca atgacatagg
    60421 tggcgctgga accgtattca cccgccatcc ccatgccgat gaccagacgc gcgataaaca
    60481 tggtgatgta gcctggcgca aagccgcagg ccagcgtccc ggccgagaag agaacgatgc
    60541 tggtgaccat tgccagacga cgcccgtagc ggtcacccat agcgccgagc atcaggccgc
    60601 cgaaccagcg agagataaag gctgcggaga tcagacttgc cgcctgcacc gtcgtcagcc
    60661 cgaattcacc ttgtacttcg gtgagtacca gggcgattaa aacgaaatca aaaccgtcaa
    60721 gcagatatcc caaccaggca gcggaaaatg cccgccattg tgcacgattg agatggcgat
    60781 accacgggat attctgggtt gtagtactca ttgtgagtct cccgcggtgg gcgatgccca
    60841 cacgctttgg tatgaaaatt gtagggtaca gatgcgttta tttcccctca cccggtaggg
    60901 gcgagcgagg ggaaaacaac tcacccgcgc tcttgcatca actgctgggc cagcgccttc
    60961 agttctggca gatatttttc atctaccggt ccaaacggtt tgcggcacag cggcacagaa
    61021 acgacatcca tataatggag gacagttttc aggccgcgga atacgcccgt tttgatcagt
    61081 aaatcaatga ctttattgca ttcagtttgc agtttctgcg cggtctggat atcgccttct
    61141 ttcagcgcct taacgatccc ctgatagcgc cagcccatga tgttgtaggt actaccgata
    61201 ccaccatcag cgcccgccag cagaccagag gcgaagattt cgtcgtaacc gttatagagc
    61261 acaagatcag gatgttcacg acggatctgc tccatctgat agagatcgcc agaagtctgt
    61321 ttcagcgcac cgacgccagg caacgtaacc agggtgttga tctgatccag ggtcagtttt
    61381 accccactca gggctggaat gttgtacacc accatcggca aaccatccgc tgaatcaata
    61441 attgcccgat agtgatcgca gtgttcttca aagctgaaag gatagtagaa cggcgtgacg
    61501 gcggagacgg catcgaagcc ataacgttta gccgatgccg caagttgttg gctttcggcg
    61561 gtgctgacgc aaccgacgtg ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg
    61621 atttccagta cctgttcacg ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca
    61681 cccacgtata aaccgtcgat gccctgctga atattgaact gaaccaggcg acgcagactc
    61741 gctttatcca gtgcttgttg ttgatcaaaa ggagtcagga gtgcagccat tacgccacgt
    61801 aaattcgttg ccataaatac ctctgaagtg atgcttgtct gataaacgat atacctttat
    61861 acctgttata ccagatcaat taagcaacac cccatacaga aagcttataa tgcgatctgc
    61921 ttcactaaag tggcattatt tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc
    61981 agagacgctg ttgagatgcg attgcaacgc acgatcggct tcgtcaggat catgacggcg
    62041 gatcgcatca acgatcgcaa tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc
    62101 ctgatcggta accgttgggc gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc
    62161 catgaagatt gggttaccgg ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg
    62221 aatgaatgcc gcgttgttat ccagcgactg actgttaatt tccagtgctt ttgccagcaa
    62281 atcgatttgc tcatcggtgg catgttcagc cgcatagcgc accaggctgg attcaaagaa
    62341 cagacgtaat tgttcgaaat gggcaatccc accaggatga gaaaggaaat ctttcgccat
    62401 gccggaaagc tcgccgatga tggtgtccgc agaaggacgt gagacgcggg cacgttcgcc
    62461 gttgttgatt tgcaccagac ctttgcgttt taacgctgcc agcgcttcgc gcacagaagg
    62521 acgcccgacg ttaaagaacg ccatcagttc gcgttcagac ggtaattgtt caccttcgcc
    62581 aaattcacga cggcggatca tctgttccag ctcttcttcc accatttcgg agagtttttt
    62641 acgcgccagc gggcggctac gcaagttgcg accaattgca ggtgaagaat cttcggtttg
    62701 cgaatcaaat gcgttcataa ggcccattct gtaaggtcag tgtgattaac atcatcagtg
    62761 acatcctatc acaggattga aagtagggga aaatggcagg tttttctctt tgtgcctcat
    62821 cattaccata attaacggaa taattaacta ttgcgaaaaa ttaatgtaac gcagataaaa
    62881 acat
//
LOCUS       DAZFRJ010000006        58548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000006 DAZFRJ010000000
VERSION     DAZFRJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.006"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..464)
                     /gene="folK"
                     /locus_tag="ACHKUN_000329"
     CDS             complement(<1..464)
                     /gene="folK"
                     /locus_tag="ACHKUN_000329"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO6658653.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFD"
     gene            complement(461..1858)
                     /gene="pcnB"
                     /locus_tag="ACHKUN_000330"
     CDS             complement(461..1858)
                     /gene="pcnB"
                     /locus_tag="ACHKUN_000330"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO6658654.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1918..2814)
                     /gene="gluQRS"
                     /locus_tag="ACHKUN_000331"
     CDS             complement(1918..2814)
                     /gene="gluQRS"
                     /locus_tag="ACHKUN_000331"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO6658655.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2881..3336)
                     /gene="dksA"
                     /locus_tag="ACHKUN_000332"
     CDS             complement(2881..3336)
                     /gene="dksA"
                     /locus_tag="ACHKUN_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO6658656.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3514..4218)
                     /gene="sfsA"
                     /locus_tag="ACHKUN_000333"
     CDS             complement(3514..4218)
                     /gene="sfsA"
                     /locus_tag="ACHKUN_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO6658657.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4233..4763)
                     /gene="thpR"
                     /locus_tag="ACHKUN_000334"
     CDS             complement(4233..4763)
                     /gene="thpR"
                     /locus_tag="ACHKUN_000334"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO6658658.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4837..7266
                     /gene="hrpB"
                     /locus_tag="ACHKUN_000335"
     CDS             4837..7266
                     /gene="hrpB"
                     /locus_tag="ACHKUN_000335"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO6658659.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7398..9932
                     /gene="mrcB"
                     /locus_tag="ACHKUN_000336"
     CDS             7398..9932
                     /gene="mrcB"
                     /locus_tag="ACHKUN_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO6658660.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10152..12395
                     /gene="fhuA"
                     /locus_tag="ACHKUN_000337"
     CDS             10152..12395
                     /gene="fhuA"
                     /locus_tag="ACHKUN_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO6658661.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12446..13243
                     /gene="fhuC"
                     /locus_tag="ACHKUN_000338"
     CDS             12446..13243
                     /gene="fhuC"
                     /locus_tag="ACHKUN_000338"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO6658662.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13243..14133
                     /gene="fhuD"
                     /locus_tag="ACHKUN_000339"
     CDS             13243..14133
                     /gene="fhuD"
                     /locus_tag="ACHKUN_000339"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO6658663.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14130..16112
                     /gene="fhuB"
                     /locus_tag="ACHKUN_000340"
     CDS             14130..16112
                     /gene="fhuB"
                     /locus_tag="ACHKUN_000340"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO6658664.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVHAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16147..17427)
                     /gene="hemL"
                     /locus_tag="ACHKUN_000341"
     CDS             complement(16147..17427)
                     /gene="hemL"
                     /locus_tag="ACHKUN_000341"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO6658665.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17593..17640
                     /gene="yadX"
                     /locus_tag="ACHKUN_000342"
     CDS             17593..17640
                     /gene="yadX"
                     /locus_tag="ACHKUN_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO6658666.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17652..19073
                     /gene="clcA"
                     /locus_tag="ACHKUN_000343"
     CDS             17652..19073
                     /gene="clcA"
                     /locus_tag="ACHKUN_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO6658667.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19097..19162
                     /gene="yadW"
                     /locus_tag="ACHKUN_000344"
     CDS             19097..19162
                     /gene="yadW"
                     /locus_tag="ACHKUN_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO6658668.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19155..19499
                     /gene="erpA"
                     /locus_tag="ACHKUN_000345"
     CDS             19155..19499
                     /gene="erpA"
                     /locus_tag="ACHKUN_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO6658669.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19546..20169)
                     /gene="yadS"
                     /locus_tag="ACHKUN_000346"
     CDS             complement(19546..20169)
                     /gene="yadS"
                     /locus_tag="ACHKUN_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO6658670.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20207..21007)
                     /gene="btuF"
                     /locus_tag="ACHKUN_000347"
     CDS             complement(20207..21007)
                     /gene="btuF"
                     /locus_tag="ACHKUN_000347"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO6658671.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21000..21698)
                     /gene="mtnN"
                     /locus_tag="ACHKUN_000348"
     CDS             complement(21000..21698)
                     /gene="mtnN"
                     /locus_tag="ACHKUN_000348"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO6658672.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21782..23299
                     /gene="dgt"
                     /locus_tag="ACHKUN_000349"
     CDS             21782..23299
                     /gene="dgt"
                     /locus_tag="ACHKUN_000349"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO6658673.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23429..24853
                     /gene="degP"
                     /locus_tag="ACHKUN_000350"
     CDS             23429..24853
                     /gene="degP"
                     /locus_tag="ACHKUN_000350"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO6658674.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25010..26161
                     /gene="cdaR"
                     /locus_tag="ACHKUN_000351"
                     /pseudo
     CDS             25010..26161
                     /gene="cdaR"
                     /locus_tag="ACHKUN_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26250..26636)
                     /gene="yaeH"
                     /locus_tag="ACHKUN_000352"
     CDS             complement(26250..26636)
                     /gene="yaeH"
                     /locus_tag="ACHKUN_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO6658675.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26798..27610)
                     /gene="yaeI"
                     /locus_tag="ACHKUN_000353"
     CDS             complement(26798..27610)
                     /gene="yaeI"
                     /locus_tag="ACHKUN_000353"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO6658676.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27665..28489)
                     /gene="dapD"
                     /locus_tag="ACHKUN_000354"
     CDS             complement(27665..28489)
                     /gene="dapD"
                     /locus_tag="ACHKUN_000354"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO6658677.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28520..31192)
                     /gene="glnD"
                     /locus_tag="ACHKUN_000355"
     CDS             complement(28520..31192)
                     /gene="glnD"
                     /locus_tag="ACHKUN_000355"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO6658678.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31254..32048)
                     /gene="map"
                     /locus_tag="ACHKUN_000356"
     CDS             complement(31254..32048)
                     /gene="map"
                     /locus_tag="ACHKUN_000356"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO6658679.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32416..33141
                     /gene="rpsB"
                     /locus_tag="ACHKUN_000357"
     CDS             32416..33141
                     /gene="rpsB"
                     /locus_tag="ACHKUN_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO6658680.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33399..34250
                     /gene="tsf"
                     /locus_tag="ACHKUN_000358"
     CDS             33399..34250
                     /gene="tsf"
                     /locus_tag="ACHKUN_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO6658681.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34397..35122
                     /gene="pyrH"
                     /locus_tag="ACHKUN_000359"
     CDS             34397..35122
                     /gene="pyrH"
                     /locus_tag="ACHKUN_000359"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO6658682.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35414..35971
                     /gene="frr"
                     /locus_tag="ACHKUN_000360"
     CDS             35414..35971
                     /gene="frr"
                     /locus_tag="ACHKUN_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO6658683.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36063..37259
                     /gene="ispC"
                     /locus_tag="ACHKUN_000361"
     CDS             36063..37259
                     /gene="ispC"
                     /locus_tag="ACHKUN_000361"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO6658684.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37448..38206
                     /gene="ispU"
                     /locus_tag="ACHKUN_000362"
                     /gene_synonym="uppS"
     CDS             37448..38206
                     /gene="ispU"
                     /locus_tag="ACHKUN_000362"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO6658685.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38219..39076
                     /gene="cdsA"
                     /locus_tag="ACHKUN_000363"
     CDS             38219..39076
                     /gene="cdsA"
                     /locus_tag="ACHKUN_000363"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6658686.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39088..40440
                     /gene="rseP"
                     /locus_tag="ACHKUN_000364"
     CDS             39088..40440
                     /gene="rseP"
                     /locus_tag="ACHKUN_000364"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO6658687.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40470..42902
                     /gene="bamA"
                     /locus_tag="ACHKUN_000365"
     CDS             40470..42902
                     /gene="bamA"
                     /locus_tag="ACHKUN_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO6658688.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43024..43509
                     /gene="skp"
                     /locus_tag="ACHKUN_000366"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43024..43509
                     /gene="skp"
                     /locus_tag="ACHKUN_000366"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO6658689.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43513..44538
                     /gene="lpxD"
                     /locus_tag="ACHKUN_000367"
     CDS             43513..44538
                     /gene="lpxD"
                     /locus_tag="ACHKUN_000367"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO6658690.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44643..45098
                     /gene="fabZ"
                     /locus_tag="ACHKUN_000368"
     CDS             44643..45098
                     /gene="fabZ"
                     /locus_tag="ACHKUN_000368"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO6658691.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45102..45890
                     /gene="lpxA"
                     /locus_tag="ACHKUN_000369"
     CDS             45102..45890
                     /gene="lpxA"
                     /locus_tag="ACHKUN_000369"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO6658692.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45890..47038
                     /gene="lpxB"
                     /locus_tag="ACHKUN_000370"
     CDS             45890..47038
                     /gene="lpxB"
                     /locus_tag="ACHKUN_000370"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO6658693.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47035..47631
                     /gene="rnhB"
                     /locus_tag="ACHKUN_000371"
     CDS             47035..47631
                     /gene="rnhB"
                     /locus_tag="ACHKUN_000371"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO6658694.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47668..51150
                     /gene="dnaE"
                     /locus_tag="ACHKUN_000372"
     CDS             47668..51150
                     /gene="dnaE"
                     /locus_tag="ACHKUN_000372"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO6658695.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51163..52122
                     /gene="accA"
                     /locus_tag="ACHKUN_000373"
     CDS             51163..52122
                     /gene="accA"
                     /locus_tag="ACHKUN_000373"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO6658696.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52221..54362
                     /gene="ldcC"
                     /locus_tag="ACHKUN_000374"
     CDS             52221..54362
                     /gene="ldcC"
                     /locus_tag="ACHKUN_000374"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO6658697.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54419..54808
                     /gene="yaeR"
                     /locus_tag="ACHKUN_000375"
     CDS             54419..54808
                     /gene="yaeR"
                     /locus_tag="ACHKUN_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6658698.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54873..56171
                     /gene="tilS"
                     /locus_tag="ACHKUN_000376"
     CDS             54873..56171
                     /gene="tilS"
                     /locus_tag="ACHKUN_000376"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO6658699.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56220..56480)
                     /gene="rof"
                     /locus_tag="ACHKUN_000377"
     CDS             complement(56220..56480)
                     /gene="rof"
                     /locus_tag="ACHKUN_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO6658700.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56467..56667)
                     /gene="yaeP"
                     /locus_tag="ACHKUN_000378"
     CDS             complement(56467..56667)
                     /gene="yaeP"
                     /locus_tag="ACHKUN_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO6658701.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56833..57378
                     /gene="yaeQ"
                     /locus_tag="ACHKUN_000379"
     CDS             56833..57378
                     /gene="yaeQ"
                     /locus_tag="ACHKUN_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO6658702.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57375..57797
                     /gene="arfB"
                     /locus_tag="ACHKUN_000380"
     CDS             57375..57797
                     /gene="arfB"
                     /locus_tag="ACHKUN_000380"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO6658703.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57811..58521
                     /gene="nlpE"
                     /locus_tag="ACHKUN_000381"
                     /gene_synonym="cutF"
     CDS             57811..58521
                     /gene="nlpE"
                     /locus_tag="ACHKUN_000381"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO6658704.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13739 a        14542 c        16308 g        13959 t
ORIGIN      
        1 ttgtcaaatg ctcttgtatg taagacttca cgcaacgtct ccccatcagg aaacgccagc
       61 tccggtgcaa tctcaaacag cggccacagc ataaatccac gattcttcat atcgtagtgc
      121 ggaacggtca ggcgttcggt atttatcact tcattaccaa acagcatgat atcgagatcc
      181 agcgtgcgtg gtccccagcg ttcagcttta cgaacacgac cctgttgcag ttctatgcgc
      241 tgtgtatggt tgagtaactc ttccggggcg agcgaagttt caagcgccac ggcggcgttg
      301 aggtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga agaaacggca
      361 agaatgtggc tttcagggat atcgcctaat gctttcaggg cagcattgac ctgctccagc
      421 ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta ccctcacgac
      481 gcggtgcgcg tttgcgtgga cgacgagtac ggcgacgcgg tgacggctct tcatccaact
      541 cgttaagcat ccctttttga tctggtggcg cggaaacctg gaactcaccc caccatttca
      601 ccagacgctg cagttcagcg ttacgttcaa cttcagctcg caaggccaac aggtcataag
      661 ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg tttaccctga cgacgggaca
      721 tacgcaactg caactgccag atatcgcggg ttaacgtcgt cagacgtttc gggattgcca
      781 gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg
      841 tcaggccgct ttcctgggca atcttctgtg ccgtctccag cagtgggtac cagaacatgg
      901 cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct
      961 tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg aagtagcggg
     1021 taatggtcgg gaacagcggc tggaacagat gatattcaca caacagctta taggtatcgt
     1081 aaccgtagcc cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc ggtgggatat
     1141 cgttcaacag ggtagcgagg cgagggatcg gttctgcggt ttccgggctg atgcgcatac
     1201 ccaatttggc ggcaaaacgt accgcgcgca gcatacgtac cggatcttca cggtagcgcg
     1261 tttccgggtt accaatcaga cggataacgc cgtccttcag atccttcata ccgccaacgt
     1321 aatcacggac ggtaaaatcc gctacgctgt aatagaggct gttgatagtg aaatcgcggc
     1381 gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc
     1441 cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa
     1501 cttcgataat ctccgggcca aacattacat gagccagacg gaaacggcga cccaccaggc
     1561 ggcagttacg gaacagtttg cgcacctgct caggcgtggc gttagtggtt acgtcaaaat
     1621 ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt
     1681 atcccgcttt gttgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg
     1741 aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag
     1801 cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag
     1861 tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca
     1921 gcatgacgca tttgagaatg ttgaatttac aattgccgac tcgggcacgg cggtaagccg
     1981 ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc
     2041 tgcctgctgc cccagaaatt gaagtgccgc gattagtacc gggcgtggat cgcctttcgg
     2101 caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg
     2161 cagatgaatg taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccttac
     2221 tgttggttca atcagatcag ccccacgcac tatttctgta acgccctgga aatgatcatc
     2281 aaccacaaca gccaggttgt aggcgaacaa cccatcacgg cgatgaatga taaaatcttc
     2341 ccgtgccagt ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac
     2401 cggatgctgc tggcggatac gcactgcggc attgtctggt ccatgatgca acacccggca
     2461 atgaccgtcg taaataccgc caatgctttg aatacgcgca cgcgtacagg tgcagtaata
     2521 acttagtcct tgttcatgta accaggcgag tgcttcacga taggcgtggt gacgttgcga
     2581 ttgccagaga acatcgccgt cccagtgcag accgtaatgt tccagctggc gcaggatagt
     2641 ttctgcggca ccgggaactt cacgaggcgg gtcgatatct tctatgcgta ccagccagcg
     2701 accttgccgg gcgcgagcct gcaaatagct gccgagcgcg gcgatcagag agccaaaatg
     2761 aagctcgccg gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt
     2821 gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga acggctgtaa
     2881 ttagccagcc atctgttttt cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc
     2941 ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg attcgcagta
     3001 gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagcttacg
     3061 ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac
     3121 cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc
     3181 cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt
     3241 catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact
     3301 cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta
     3361 tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat
     3421 agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg
     3481 accagaatgt aaattaacca gttacttact ttactacaat gtaaccggca gtgatttttt
     3541 aagagccatg ccttcagcag aaatttccgc tttgtaagcc agaatttcta cccccctctg
     3601 ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg
     3661 tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac
     3721 gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc
     3781 ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc
     3841 cgcctgcaac ataaagtcaa tacggctgcg ttctgcgccg tattttactt cgcttttcag
     3901 cgagctatag cctgacagtt ctgaaattga ttcattaagg atagcctctt tcgtcaacct
     3961 gttagcccaa agcgtgttga cgcaaataaa tgcgccgctc tggctttgag ttaattccca
     4021 ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg
     4081 cgttgcacaa ccggtcattg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc
     4141 gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag
     4201 agggggagaa aattccattg cgacaatcct tgttattgcg ttagcgccca gcgttttagc
     4261 ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc
     4321 gcatacgacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat
     4381 aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt
     4441 gaacggagca tattcgccag ctggattaag ccgcgtggcg gctgacgcat ccctaaccac
     4501 accacacgcg aacgcagcca ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga
     4561 cgaatccgtc cggctaaaag agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa
     4621 aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aggtgggaag
     4681 tgtgtggcgc gccagtggat aatctgttcg cggatttctg caggtaagtc gatagcaaag
     4741 aacagacgtt gcggttcaga catgtgaggc actcggttat gaattaccgc gatgctacaa
     4801 tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc
     4861 gtcttacctg aattacttac cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg
     4921 accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac
     4981 gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacggctg
     5041 gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac
     5101 tgcgtcgggc cgaatacccg cctggaagta gttaccgaag gcgtgctgac gcggatgatc
     5161 cagcgtgacc cggagttgag cggtgttgga ttggtgatcc tcgatgagtt tcatgagcgc
     5221 agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat
     5281 gaccttaaac tgctgattat gtcggctacg ctggacaacg accgcttgca gcaaatgctg
     5341 ccagaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg ccgttattta
     5401 ccactgccca cgcatcagcg ttttgacgaa gccgttgcgg tagccaccgc tgaaatgctg
     5461 cgtcaggaaa gtggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg
     5521 caggaacaac tggcttcgcg catcggcagc gatgtattgc tctgcccgct gtatggcgcg
     5581 ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg
     5641 gtgctggcga ccaatattgc tgaaaccagt ttaaccatcg aaggtattcg tctggtggtg
     5701 gattgtgccc aggagcgtgt ggcgcgtttt gatccgcgca cggggcttac gcgactgatt
     5761 actcaacgcg ttagccaggc atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag
     5821 ccgggtatct gcctgcattt aatcgccaag gaacaagcag aacgcgccgc ggcgcaaagt
     5881 gaaccggaga tattacaaag cgatctttcc ggtttgctga tggaattact gcaatgggga
     5941 tgcagcgatc cggcgcagat gagctggctg gatcaaccgc cagtagtgaa tctactggcc
     6001 gcgaaacgtc tgttacaaat gctgggggca ctggagggtg aacggcttag tgcgcaaggg
     6061 caaaaaatgg cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcgaag
     6121 aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg
     6181 atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt
     6241 agtcagcaac tgttaaaacg cttaaacgta cgtggcggtg aggcagacag ttcgcttatc
     6301 gcgccgctac ttgccggggc gtttgccgat cgcattgctc gtcgccgtgg gcaagatgga
     6361 cgctatcaac tggcaaacgg catgggagcg atgctcgatg ccaacgacgc gctaagccgc
     6421 cacgaatggt tgatcgcacc gttattattg cagggcagcg cctcgccgga tgcgcggatt
     6481 ttactggcgc tgccggtcga tattgatgag ttagtacaac gctgcccgca gctggtacag
     6541 cagtctgaca ctgtggagtg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg
     6601 caaatcggtc tgctgacggt gaaagtgcag ccgctggcga aaccctcgga agacgagttg
     6661 catcaggcga tgcttaatgg catccgcgat aaagatttaa gcgtgctcaa ctggacggcg
     6721 gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac
     6781 tggccagcgg ttgatgatga aagtttattg gcgacgctgg aaacgtggct gctgccacat
     6841 atgactggcg tacattcact acgcggcctg aaatcactcg acatttatca ggcactacgc
     6901 ggattacttg attggggaat gcagcaacgt ctggatagtg aattgcctgc gcattacact
     6961 gtgccgacgg gaagccggat cgccattcgt tatcatgaag ataacccgcc cgcgctggcg
     7021 gtgagaatgc aagagatgtt tggcgaggcc accaatccga cgatcgccca ggggcgcgtg
     7081 ccgctggtgc tggagttgct ttcacctgcc caaaggccat tacaaatcac acgagatttg
     7141 agcgccttct ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc
     7201 aaacatgtct ggccggacga cccggcaaat actgcaccga cgcgacggac gaaaaagtat
     7261 tcgtaaggtt ggttttctcc ctctccctgt gggagagggg accgatcgag cacaaatttt
     7321 gagagatatc ttcttctgtc ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa
     7381 tattgcggag aaaaagcatg gccgggaatg accgcgagcc aattggacgc aaagggaaac
     7441 cgacgcgtcc ggtcaaacaa aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg
     7501 acgattatga tgactatgag gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca
     7561 aagggcgtaa gcctcgtggc aaacgcggct ggctatggct gctgttaaaa ctggctatcg
     7621 tttttgccgt gctgatcgcc atttacggcg tttatctcga tcaaaaaatt cgtagccgta
     7681 ttgatggcaa ggtctggcaa ctgcctgcgg cagtttatgg ccgaatggtc aaccttgagc
     7741 cagatatgac catcagcaag aacgagatgg tgaagctgct ggaggcgact cagtatcgtc
     7801 aggtgtcgaa aatgacccgt cctggcgaat ttaccgtgca ggccaacagc attgagatga
     7861 tccgccgtcc gtttgatttc ccggacagta aagaagggca ggtgcgtgcg cgtctgacct
     7921 ttgatggcga tcatctggcg acgatcgtca atatggagaa caaccgtcag ttcggtttct
     7981 tccgtcttga tccgcgtctg atcaccatga tctcctcgcc aaacggtgag cagcgtttgt
     8041 ttgtgccgcg cagtggtttc cctgatttgc tggtggatac tttgctggcg acagaagacc
     8101 gtcattttta cgagcatgac ggcatcagcc tctactccat cggacgtgcg gtgctggcaa
     8161 acctgaccgc cggacgcacg gtgcagggag cgagtacgct gacgcaacag ctggtgaaaa
     8221 acctgttcct ctccagtgag cgttcttact ggcgtaaagc gaacgaagct tacatggcgc
     8281 tgatcatgga cgcgcgttac agcaaagacc gtattcttga gctgtatatg aacgaggtgt
     8341 atctcggtca gagcggcgac aacgaaatcc gcggcttccc gctggcgagc ttgtattact
     8401 ttggtcgccc ggtagaagag ctaagcctcg accagcaggc gctgttagtc ggcatggtga
     8461 aaggagcgtc catctacaac ccgtggcgta acccaaaact ggcgctggag cgacgtaatc
     8521 tggtgctgcg tctgctgcaa cagcaacaga ttattgatca agaactctat gacatgttga
     8581 gtgcacgtcc gctgggtgtt cagccacgcg gtggggtgat ctctccacag ccagccttta
     8641 tgcaactggt gcgtcaggag ctgcaggcga aattaggcga taaggtaaaa gatctctccg
     8701 gcgtgaagat cttcactacc tttgactcgg tggcccagga cgcggcagaa aaagccgccg
     8761 tggaaggcat tccggcactg aagaaacagc gtaagttgag cgatcttgaa actgcgattg
     8821 tggtcgtcga ccgctttagt ggtgaagttc gtgcgatggt cggtggttct gagccgcagt
     8881 ttgcgggcta caaccgtgcg atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag
     8941 cgacttatct gactgcctta agccagccga aaatctatcg tctgaatacg tggattgcgg
     9001 atgcgccaat cgcgctgcgt cagccgaatg gccaggtctg gtcaccgcag aatgatgacc
     9061 gtcgttatag cgaaagcggc agagtgatgc tggtggatgc gttgacccgt tcgatgaacg
     9121 tgccgacggt aaatctgggg atggcgctgg ggctgcctgc ggttacggag acctggatca
     9181 aactgggcgt accgaaagat cagttgcatc cggttccggc gatgctgctg ggggcgttga
     9241 acttaacgcc aatcgaagtg gcgcaggcgt tccagaccat cgccagcggc ggtaaccgtg
     9301 caccgctttc tgcactgcgt tcggtaattg cggaagatgg caaggtgctg tatcagagct
     9361 tcccgcaggc ggaacgcgct gttccggcgc aggcggcgta tctgacacta tggaccatgc
     9421 agcaggtggt acaacgcggt acgggtcgtc agcttggggc gaaatacccg aacctgcatc
     9481 tggcagggaa aacagggact accaacaata acgtagatac ctggtttgca ggcattgacg
     9541 gcagcacggt gaccatcacc tgggtaggcc gtgataacaa ccagccgacc aaactgtatg
     9601 gagccagcgg ggcaatgtcg atttatcagc gttatctggc taaccagacg ccaacgccgc
     9661 tgaatcttgt tccgccagaa gatattgcag atatgggcgt ggactacgac ggcaactttg
     9721 tttgcagcgg tggcatgcgt gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc
     9781 agcagagcga gatgcagcag cagccgtcag gcaatccgtt tgatcagtct tctcagccgc
     9841 agcaacagcc gcaacagcaa cctgctcagc aagagcagaa agacagcgac ggtgtagccg
     9901 gttggatcaa ggatatgttt ggtagtaatt aacatctaag cgtgaaatac cggatggcga
     9961 gttgccatcc ggtaaaataa catcccatct aagatattaa ccctttcttt tcatctggtt
    10021 gtttattaac ccttcaggaa cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc
    10081 gaatattatc ttatctttat aataatcatt ctcgtttacg ttatcattca ctttacatca
    10141 gagatatacc aatggcgcgt tccaaaactg ctcagccaaa acactcactg cgtaaaatcg
    10201 cagttgtagt agccacagcg gtaagcggca tgtctgttta tgcacaggca gcggttgaac
    10261 cgaaagaaga cactatcacc gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc
    10321 ctgctgcaac tattgcggcg cgacagtctg ctaccggcac taaaaccgat acgccgattc
    10381 aaaaagtgcc acagtctatt tctgttgtga ccgccgaaga gatggcgctg catcagccga
    10441 agtcggtaaa agaagcgctt agctacacgc cgggtgtctc tgttggtacg cgtggcgcat
    10501 ccaacaccta tgaccacctg atcattcgcg gctttgcggc agaaggccaa agccagaata
    10561 actatctgaa tggcctgaag ttgcagggca acttctataa cgatgcggtc attgacccgt
    10621 atatgctgga acgcgctgaa attatgcgtg gcccggtttc cgtgctttac ggtaaaagca
    10681 gtcctggcgg cctgttgaat atggtcagca agcgtccgac caccgaaccg ctgaaagaag
    10741 ttcagtttaa agccggtact gacagcctgt tccagactgg ttttgacttt agcgattcgt
    10801 tggatgatga cggtgtttac tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc
    10861 agcagaaagg gtcagaagag cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg
    10921 atgataaaac caatttcacc ttcctttctt acttccagaa cgagccggaa accggttatt
    10981 acggctggtt gccgaaagag ggaaccgttg agccgctgcc gaacggtaag cgtctgccga
    11041 cagactttaa tgaaggggcg aagaacaaca cctattctcg taatgagaag atggtcggct
    11101 acagcttcga tcacgaattt aacgacacct ttactgtgcg tcagaacctg cgctttgctg
    11161 aaaacaaaac ctcgcaaaac agcgtttatg gttacggtgt ctgctccgat ccggcgaatg
    11221 cttacagcaa acagtgtgcg gcattagcgc cagcggataa aggccattat ctggcacgta
    11281 aatacgtcgt tgatgatgag aagctgcaaa acttctccgt tgatacccag ttgcagagca
    11341 agtttgccac tggcgatatc gaccacaccc tgctgtccgg tgtcgacttt atgcgtatgc
    11401 gtaatgacat caacgcctgg tttggttacg acgactctgt gccactgctc aatctgtaca
    11461 atccggtgaa taccgatttc gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc
    11521 gcattctgaa taaacagaaa caaacgggcg tttatgttca ggatcaggcg cagtgggata
    11581 aagtgctggt caccctaggc ggtcgttatg actgggcaga tcaagaatct cttaaccgcg
    11641 ttgctgggac gaccgataaa cgtgatgaca aacagtttac ctggcgtggt ggtgttaact
    11701 acctgtttga taatggtgta acaccttact tcagctatag cgaatcgttt gaaccttctt
    11761 cgcaagttgg gaaggatggt aatattttcg caccgtctaa aggtaagcag tatgaagtcg
    11821 gcgtgaaata tgtaccggaa gatcgtccga ttgtagttac tggtgccgtg tataatctca
    11881 ctaaaaccaa caacctgatg gcggaccctg agggttcctt cttctcggtt gaaggtggcg
    11941 agatccgcgc acgtggcgta gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg
    12001 tagtcggttc ttatacttac accgatgcgg aatacaccac cgatactacc tataaaggca
    12061 atacgcctgc acaggtgcca aaacacatgg cttcgctgtg ggctgactat accttctttg
    12121 acggtccgct ttcaggtctg acgctgggca ccggtggtcg ttatactggc tccagctatg
    12181 gtgatccggc taactccttt aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt
    12241 atgatctggc gcgagtcggc atggcgggct ccaacgtggc gctgcatgtt aacaacctgt
    12301 tcgatcgtga atacgtcgcc agctgcttta acacttatgg ctgcttctgg ggcgcagaac
    12361 gtcaggtcgt tgcaaccgca accttccgtt tctaatttct cttttggggc acggatttcc
    12421 gtgcccattt cacaagttgg ctgttatgca ggaatacacg aatcattccg ataccacttt
    12481 tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt
    12541 aacctttcct gccgggaaag tgaccggtct gattggtcac aacggttctg gtaaatccac
    12601 tctgctcaaa atgcttggcc gtcatcagcc tccgtcagaa ggggagattc ttcttgatgc
    12661 ccagccgctg gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt atttgccgca
    12721 gcagcttcct ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc
    12781 gtggcatggc gcgctggggc gctttggtgc ggcagatcgc gaaaaggtcg aggaagctat
    12841 ctcgctggtt ggcttaaaac cgctggcgca tcggctggtc gatagtctct ctggcggcga
    12901 acgtcagcgg gcgtggatcg ccatgctggt ggcgcaggat agccgttgtc tgttgctcga
    12961 cgaaccgact tcggcgctgg atatcgccca ccaggttgat gtgctggcgc tggtgcaccg
    13021 tttaagtcag gaacgtggcc tgacggtcat tgccgtgctg cacgatatca atatggcggc
    13081 acgctactgt gattatctgg tcgccctgcg cggtggtgaa atgattgctc agggaacgcc
    13141 tgcggaaatt atgcgcggcg aaaccctcga aatgatttat ggcatcccga tgggtatttt
    13201 gccgcatccg gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg cttacctctt
    13261 atttcgcgcc gtcgactgtt aacggcgatg gcgctttctc cgttgttatg gcagatgaat
    13321 accgcccacg cggcggctat tgaccccaat cgtattgtgg cgctggagtg gttgccggtg
    13381 gaattactgc tggcgctcgg catcgtgcct tacggcgtgg cggataccat caactatcgc
    13441 ctgtgggtca gcgaaccacc attgccggac tcagtgattg acgtcggttt gcgtacagaa
    13501 cctaaccttg aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat
    13561 ggcccttcac cagaaatgct ggcacgtatt gcgccgggtc gcggatttaa cttcagtgac
    13621 ggcaaacagc cgttggcgat ggcgcgtaaa tcgctgacgg aaatggcaga tttacttaac
    13681 ctgcaaagcg cagcagaaac gcatttagcg cactatgaag actttatccg cagcatgaaa
    13741 ccccgctttg tgaagcgtgg tgcacgcccg ttactgctga cgacgcttat cgatccgcgc
    13801 catatgctgg tcttcggtcc aaacagcttg ttccaggaaa ttctcgatga gtacggcatc
    13861 ccaaatgcct ggcaagggga aaccaacttc tggggcagta ccgccgtcag tatcgatcgt
    13921 ctggcggcgt ataaagacgt tgatgtgctc tgttttgatc acgacaacag caaagacatg
    13981 gatgcgctaa tggcaacccc gctgtggcag gccatgccgt ttgtccgtac agggcgcttt
    14041 cagcgcgtac ccgcagtctg gttttatggt gcaacgcttt cggcaatgca ctttgtgcgt
    14101 gttctggata acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt ttcccggcgt
    14161 tattgctggc gctgttagtg attgtcgcta cggcgctaac ctggatgaac ttctcacagg
    14221 cgctgccgcg cagtcagtgg gcgcaggccg cctggtcgcc ggatattgac gtcatcgagc
    14281 agatgatttt tcactacagc ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcag
    14341 gcctggggct ggtgggcgtg ctgtttcagc aagtgctgcg taatccgctg gcggaaccga
    14401 cgacacttgg cgttgctaca ggcgcgcaac tgggaattac cgtcaccaca ctttgggcga
    14461 tcccaggtgc tatggcgagc cagtttgccg cactggtggg cgcttgtgtc gttggcttaa
    14521 tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt tacgcttatt ctcgcggggc
    14581 tggtggtgag cctttattgc ggggcaatta atcagctact ggttatcttc catcacgacc
    14641 agctgcaaag catgttcctg tggagcaccg gaacgctgac gcaaaccgac tggggcggcg
    14701 ttgagcgttt atggccgcag ttgctgggcg gcgtgatgtt gacgttattg ctacttcgcc
    14761 cgttaaccct gatggggctt gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc
    14821 tcgcgcgtct ggcagcgctg tcgctggcga ttgtcatcag tgcgctgctg gtgcacgctg
    14881 tggggattat cggctttatc gggttgttcg cgccgctgct ggcaaaaatg ctgggggcgc
    14941 ggcgtctgct gccacgactg atgctggcgt cgctgattgg ggcgctgatc ctctggcttt
    15001 ccgatcaaat catcctctgg ctgactcgcg tgtggatgga agtgtccacc ggttcggtca
    15061 ctgcgttgat cggtgcgccg ctgctactgt ggctgttgcc gcgtttacgc agcattagcg
    15121 cgccggatat gaaggtcaac gatcgtgtcg cggctgaacg ccaacatgtg ctggcgtttg
    15181 ccctcgcggg cggcgtgctg ctgttgatgg ctgtggtggt ggcgctgtcg tttggtcgtg
    15241 atgcgcacgg ctggacgtgg gcgagcgggg cgttgcttga ggatttgatg ccctggcgct
    15301 ggccgcgaat tatggcggcg ctgtttgcgg gcgtcatgct ggcggtggcg ggctgtatta
    15361 ttcagcggct gaccggaaac ccgatggcaa gcccggaagt gctggggatt agctctggcg
    15421 cagcgtttgg cgtggtgttg atgctgtttc tggtgccggg taatgccttt ggctggctgt
    15481 tacctgcagg cagtctcggc gcggcggtga cgctgttgat cattatgatc gccgccggcc
    15541 gcggtggatt ttccccacac cgcatgttac tggcggggat ggcgttaagc accgcgttca
    15601 ccatgctttt gatgatgttg caggcaagtg gtgacccgcg aatggcgcaa gtgctgacct
    15661 ggatttccgg ttcgacctac aacgcgaccg atgcgcaggt ctggcgcacg ggaattgtga
    15721 tggtgatttt gctggcgatt accccgctgt gccgccgctg gctgaccatt ttaccgctgg
    15781 gtggtgatac cgcccgagcc gtaggaatgg cgctgacgcc gacgcgaatt gcgctgctgc
    15841 tgttagcggc ttgcctgacg gcgaccgcga cgatgactat tggaccgttg agttttgttg
    15901 gtttaatggc accgcatatt gcgcggatga tgggctttcg acggacgatg ccacacatcg
    15961 taatttcggc gctggtgggt ggtttactgc tggtgttcgc tgactggtgt gggcggatgg
    16021 tgctgtttcc attccagatc ccagcagggc tgctgtcgac ctttatcggc gcgccatatt
    16081 ttatctattt gttgagaaag cagagccgtt aatagaatgc ctcgcccggt gggcgaggca
    16141 aaatcatcac aacttcgcaa acacccgtcg tgcagcatcg atggtgttat tgatatcttc
    16201 catgctgtgc gccacggaca taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc
    16261 ttcgtccagc atcatatgga agaaacgctt aaagcgttcc acgtcacagg ccatcacatc
    16321 ctgatagcac gtcacggact cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg
    16381 gttaacgacc agcggaattc cggcttcttc tgccgcttcc agcagacctt ctgccagacg
    16441 tgttgtcagc tcatccagcg tttcgtgaac gcccggctgc gcgacttcat tcagacaggc
    16501 gaaacccgct gccatcgcaa ttgggttacc ggaaagcgta cccgcctgat agaccggacc
    16561 cgtcggggcc agcgcatcca ttacatcacg acgaccaccg aatgcgccta ccggcattcc
    16621 accgccgatg attttgccca ggcaggtgag atccggttcc acaccgtaat aatcctgtgc
    16681 gccagctagc gctacgcgga agccagtcat tacttcatcg atgatcagca atgcgccaaa
    16741 ttcgtcgcac agcgcgcgca gacctggcag gaactctggc agcggtggaa cgcagttcat
    16801 attgcctgcc accggctcga cgataataca ggcaatctct tgcgggtatt gctcaaacgc
    16861 ggcgcgtaca gaagccagat cgttataagt acaggttaag gtatgtttgg cgaaatctgc
    16921 cggaacaccc ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag
    16981 caggcagtca gcgtgaccgt ggtaacaacc ttcaaattta atgattttgt cgcgaccggt
    17041 aaaaccacgg gccaggcgga tggcgctcat cgtcgcttca gtgccggagt tcaccatgcg
    17101 caccatatcc atggtcggga ccagttcggt caccagttgc gccattttca cttccatttc
    17161 ggtcggtgca ccaaagctta aaccacgctc ggcggcttca atcacggcat tgcggatagc
    17221 cggatggtta tggcccagca ccatcggccc ccaggaaccg acataatcga tataggcttt
    17281 gccatcaaca tcgtacagat aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc
    17341 cacgccagta aaggcgcgaa caggggagtt cacaccgcca gggatcagct cgcgcgctgc
    17401 gctgtaaaga ttttcagact tactcatgga gggttcctga ttcgtagaaa aagtgaatgg
    17461 ctgctattct atgttattca taacaagtta aatactcgtc aaacatcagg ctgcttgtac
    17521 tggtgcaatc ctgatttctt agagtataaa agttttgtgc atttgaaaca ttacgctttg
    17581 caaaggattt tcatggaacg tgcgagtaaa atgccgtcat cttatttgta tgaccaataa
    17641 gtgatcattg gatgaaaact gatactccct ctttagaaac accgcaggcc gcgcgcctgc
    17701 gacgcagaca actgattcgc caacttcttg agcgcgataa aaccccgtta gccattttgt
    17761 ttatggcggc agtcgtcggc acgcttgtcg ggctggcagc ggttgctttt gacaaaggtg
    17821 tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca tactgctgat aattatccgc
    17881 ttctgttaac cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc tactttttgg
    17941 tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa ggggcgctgg
    18001 aagatcaacg tcccgttcgc tggtggcgtg tgttgccggt gaaattcttt ggcgggctgg
    18061 ggacactcgg cggaggcatg gtgctagggc gcgaaggacc aaccgtgcag atcggcggca
    18121 atatcggtcg catggtgctt gatattttcc gcctgaaagg tgatggagct cgccatacgc
    18181 tgctggcaac cggtgctgct gcggggctgg ctgcggcctt taacgcgccg ctggcgggta
    18241 ttttgtttat tatcgaagag atgcgtccgc agtttcgcta tacgttaatt tcgattaaag
    18301 cggtatttat tggcgtcatt atgtcgacca ttatgtaccg gatttttaat catgaagttg
    18361 cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg ctttatctga
    18421 tcctcggtat tatttttggc attttcggcc ctatttttaa taaatgggtg ctggggatgc
    18481 aggatttgct gcaccgtgtg cacggcggca atattaccaa atgggtactg atgggtggtg
    18541 cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt
    18601 ttaacctgat tcctatcgct accgcgggga atttcagcat gggaatgctg gtgtttattt
    18661 tcgtcgctcg ggtcattacc accttactct gcttctcttc cggcgcgccg ggcggtattt
    18721 ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg
    18781 agctgtttcc gcaatatcac ctggaggcgg ggacgtttgc tattgccgga atgggggcat
    18841 tactggcggc atctattcgc gcgccgttaa cggggatcat tctggttctg gagatgaccg
    18901 ataactacca gctcattttg ccaatgatta ttaccggtct tggcgcaaca ctattagcgc
    18961 aatttaccgg cgggaaaccg ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg
    19021 aagctatgca actggcgcga agcaaggccg catcagccag cgagaatact tgaacgaaat
    19081 accagggtat tagataatgg cgattattat tgggttagaa tttgcccaat tgccgatgtc
    19141 gtttggagca aaatatgagt gatgacgtag cactgccgct ggagtttacc gacgcagcag
    19201 ccaacaaagt taaaagcctg atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt
    19261 atatcaccgg tggcggttgc agcggcttcc agtatggttt cacctttgat gatcaggtga
    19321 acgaaggcga tatgaccatc gaaaaacagg gcgttggctt ggtggttgat ccgatgagcc
    19381 tgcaatatct ggtcggcggt tccgttgact ataccgaagg actggaaggt tctcgtttca
    19441 tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt agcatctaat
    19501 ctgttgttca tgtgccggat aaactatccg gcacatcttc tctcttcaac gcccattctc
    19561 atccagcgca aacgtcggta gcttaagatg ccaacgaata gccgccagcc gaatcaagag
    19621 cgtcacgacc atgcccatca tactggctgt ttccagtggt acggaaaatg tgtaataagc
    19681 cgtagcgtgg acaataccgc cgataataca ggcagttgcg tagatttctg tacgtaaaat
    19741 catggggatt tcgcgggcca gaacatcacg aatgatcccg ccgccaacgc cagtaatgac
    19801 gcccatacaa accgcgatta acggaccggc ttccgcatta aaggctttat tcacgccaat
    19861 gccgacaaac accgccagac caacggcgtc cagcaccggc aacatccatt ttggtaagcg
    19921 tcttggctgg cgcaccagca cgatggtcag catgctggtt accattgcaa cgaccagatc
    19981 ggtgggatct ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc gaattgtccc
    20041 gccgcctaca gcggtaacca cgcccagtac cagaacacca aaagggtcca tacgcaattt
    20101 tccggctaac aaaacgccgg agatggcaaa taccgctgtg ccgactatat ccagccaata
    20161 gacgagcatt gttcaatccc cactgagcac ctgaaaaggt caggcactaa tctacctgtg
    20221 aaagcgcatt acagagctgt tgtgcagcga ggataatacg tgggcttgca cgttcaaacc
    20281 agtcactcgt gagaggaata acgggaattt tgagctgttc accccagtat tgtttgattt
    20341 taggaatttg gtccggtccg cctgtaatga caatcgcctg tggcgagcgt gctaacacct
    20401 gttcgcggct aacttgcggc cagggaaccc ggctgtcttt aaagatgttt tctccgccac
    20461 aaacttcgag tacctggttc tgaatcgact cttttccact ggtaaatggc ggattaatgc
    20521 cgaattgcag aaaaacacgt tttttaggtt tatcagcata ttgcgctttc aattgcgcgt
    20581 actgatccag cagggattgc gcggcttgtt cggccttgtc tggttgcgga ctccaggggg
    20641 ccagttgacg taacgcattg gcaatttgtt caatgcttgt cgcatcgacc cacatcactt
    20701 ttattcccag cgaagccagc tggtcaacct gccgctcggc attacctcca cgccaggcaa
    20761 tcaccagatc gggtttcagc gcgacaatgc gttccagatt catcccctgc caggtggaaa
    20821 cctgctcaat cttttgcgct tgtggaggat agtcggaata gctgctgacc ccaaccggcg
    20881 tgatcccggc ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg
    20941 cggcgttgag ccacagtggc gcaagaaaag acagggcgac cagcgccctg aacagtgact
    21001 tagccatgtg caagtttctg caccagtgac tcaaccatca ggctggactg tttagcggca
    21061 acagccagga actcatcgaa gctaagatga gactgttgat cggccacgtc ggagatggcg
    21121 cgtacgacaa caaacgggac gttgaaattg tggcagacat gggcgattgc cgtcgcttcc
    21181 atctctacag caatggcctg tgggaagttg tggcggattt tcgccagacc aacagaaccg
    21241 ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag cgttaagatt cagttcggca
    21301 atgcaggcct cagcggcagc gatcagttta tcgtcagctt taaagcctgc cggacagcct
    21361 ggtaactgac cgtattcata accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg
    21421 tccgagacaa cgatatcgcc cactttcaac gttggtgcca ggccaccggc agaaccggtg
    21481 ttaataatca catctggctt gcagtgttcc aacagcaaag tggcacccag cgccgcagcg
    21541 actttaccga tgcccgattt cagaagcgca acctcggttc cattcagttg gccggtatag
    21601 atttcgcaac cgccgagact gatagtttga cgtttttcga ttttgtcacg cagcagcgta
    21661 acttcttctt ccattgcacc aatgatgccg attttcatag atttactcgc gataagcccg
    21721 atttgaaggc atagtttacc atgcgcttac ggggaagcgt atttctcacg cgggagagga
    21781 catggcacag attgatttcc gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca
    21841 gggcgttaaa accgaacatg agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat
    21901 caactctccg gcaattcgtc gtctgcaaca aaagacgcag gtttttccac tggagcgcaa
    21961 tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg ggcgctacat
    22021 cgccaaagaa attttaagcc gtctgaaaga gcttaaattg ctggaagcat acggcctgga
    22081 tgaactgacc ggaccttttg aaagcattgt tgagatgtca tgcctgatgc acgatatcgg
    22141 caatccgccg tttggtcatt ttggcgaagc agcgataaat gactggtttc gccaacgttt
    22201 gcacccggaa gatgccgaaa gccagcctct gactgacgat cgctgcagcg tggcggcact
    22261 acgtttacgg gacggggaag aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt
    22321 atgtcatttt gaggggaatg cacaaggcat tcgtctggtg catacattga tgcggatgaa
    22381 tctcacctgg gcacaggttg gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg
    22441 cgaaacgcct gagacacatc actatttaat gaaaaagccg ggttattatc tttctgaaga
    22501 agcctatatt gcccggttgc gtaaagaact taatttggcg ctttacagtc gttttccatt
    22561 aacttggatt atggaagctg ccgacgacat ctcctattgt gtggcagacc ttgaagatgc
    22621 ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg aagcgtgggg
    22681 ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg
    22741 ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac gggtaaacac
    22801 cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc ctgcgatttt
    22861 cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa
    22921 gctatataaa aatgtcgctg taaaacatgt gtttagccat ccagatgtcg agcagcttga
    22981 attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaagcct
    23041 gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga
    23101 atcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag
    23161 taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct
    23221 gctgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct
    23281 gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg
    23341 cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac agcaattttg
    23401 cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact
    23461 ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc
    23521 agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc
    23581 agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa
    23641 tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac cgttccagag
    23701 ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac agaaattcat
    23761 ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca ccaacaacca
    23821 cgttgttgat aacgcgacgg tgattaaagt ccagctgggc gatggtcgta agttcgacgc
    23881 gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg attcagatcc agaacccgaa
    23941 aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg attacaccgt
    24001 agcgattggt aacccgtttg gcctgggaga gacggtaact tccgggattg tctctgcgct
    24061 ggggcgtagc ggcctgaatg ccgaaaacta cgaaaacttc atccagaccg atgcagcgat
    24121 caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga tcggtatcaa
    24181 caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta tcccgagcaa
    24241 catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac gcggtgagct
    24301 gggtattatg gggactgagc tgaattccga actggcgaaa gcgatgaaag ttgacgccca
    24361 gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag cgggcattaa
    24421 agcgggtgat gtgatcacct cactgaacgg taagccaatc agcagctttg ccgcactgcg
    24481 tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttgc tgcgcgacgg
    24541 taagcaggtc aacgtgaatc tggaactgca gcagagcagc cagaatcagg ttgattccag
    24601 ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt
    24661 ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga
    24721 tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct
    24781 tgacagcaaa ccgtctgtac tggcactgaa cattcagcgc ggcgacagct ccatctacct
    24841 gttaatgcag taatctccct caaccccttc ctgaaaacgg gaaggggttc tccttacaat
    24901 ctgtgaactt caccacaact ccatacatct tcatcatcct ttaggcattt gcacaatgcc
    24961 gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg gctggcatct
    25021 tgataccaaa atggcgcagg atatcgtggc acgtaccatg cgcatcatcg ataccaatat
    25081 caacgtaatg gatgcccgtg ggcgaattat cggcagcggc gatcgtgagc gtattggtga
    25141 attgcacgaa ggtgcattgc tggtactttc acagggacga gtcgtcgata tcgatgacgc
    25201 ggtggcacgt catctgcacg gtgtgcggca agggattaat ctaccgttac ggctggaagg
    25261 tgaaattgtc ggcgtaattg gcctgacagg tgaaccagag aatctgcgta aatatggcga
    25321 actggtctgc atgacggctg aaatgatgct ggaacagtcg cggttgatgc acttgttggc
    25381 tcaggatagc cgtttgcggg aggaactggt gatgaacctg attcaggcag aggagaatac
    25441 tcccgcactt actgaatggg cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt
    25501 ggctattgtt gaggtcgaca gcggtcagct tggtgtggac agcgcaatgg cggagttaca
    25561 acaactgcaa aacgcgctga ctacgcccga gcgtaataat ctggtggcga ttgtctcgct
    25621 aaccgaaatg gtggtgttga aaccggcgtt gaactctttt gggcgctggg atgcagaaga
    25681 tcatcgtaag cgagttgaac aactgattac ccgcatgaaa gagtacggcc agctgcgttt
    25741 tcgcgtttca ctgggcaact attttaccgg tcctggcagt attgcccgat cctatcgtac
    25801 ggcgaaaacg acgatggtgg tgggtaaaca gcggatgcca gaaagtcgct gctattttta
    25861 tcaggatctg atgttacctg tgttactcga cagtttgcgt ggcgactggc aggccaacga
    25921 actggcgcga ccgctggcgc ggctgaaagc gatggacaat aacggcttgc tgcgacgaac
    25981 gctggcggcg tggtttcgtc acaatgtgca accgctggca acgtcaaagg cgttgtttat
    26041 tcatcgtaat accctggagt atcggcttaa tcgtatatcg gaactgaccg ggcttgattt
    26101 gggcaatttt gatgacaggt tgctgctgta tgtggcgtta caactggatg aagagcggta
    26161 ggttatgcgt taaggttgag gcggcgctgg ctcattcccc tcagcccgaa ggggagaggg
    26221 gaatgcccga attaaccgtc aattcgggat tatttacgcg ttagtttttc cagatcggct
    26281 tcaatctcgc tgatcttatt ggttacgact gactccaggt gacgtaagtc gtcgaggatc
    26341 ttacgcttaa gatcaacttc gctgcggtca cgctggcaga tttgatcaag ctcatcaatg
    26401 atataccgta gattcgggct gatttcctgg acttctttat aaccctgacc cacaccatca
    26461 gcgacgaccg ttttacgctg acgcggatat ttaaacttaa cgctcttggc gaaaaactcg
    26521 cctttgtctt tctggaaata gattttcaga atatcgttgt tggcttcctg ccggaggctg
    26581 taacgatcaa tttcttcagg attggtaata cccagacttt tcagattgtc gtacatagcg
    26641 ttacctcaaa atgagtcagt aaattgtgct tattttagca tttggcctgg cccgcccggc
    26701 tcgactgtga tcgcagaaag cctttttctt ccagacttct atcatggcgt aaaaatcaaa
    26761 aaattacctg ctttattgtg gtgataaaat tcacgttcta caccagttcc agcatcgtta
    26821 cttccgggcg gcagttcaga cgcaaaccat acaaactgcc cacgccacgg gttgtgtaga
    26881 tgtgtcgttc gccaaaggca tttaatccgg cgacgtaacg tttatcttcg acaggggcaa
    26941 aaggttcacc aaccaacggt acgcgcagtt gcccgccgtg agtatggccg cacagcatca
    27001 gatcccaggg ttcatcacgc atgacttctt tgctgtcggg attatgcgcc agcaccagtc
    27061 ttggcagatt ggcttcgctg gcgggaggcg gtttgcattg tccggcccat aagtcaccgg
    27121 tgcctaccag ttcgaattgc ctgttcggcg tggcgatcac cgtagcctgg ttaaacaaca
    27181 ccgtgatgcc cgctgatttc aacgtctcgc caattaagtg atttttttct gtaccaacag
    27241 ggcgatcgtg gttgccgaag caggcaaacg tcggcgcaca ttcggcaagg ggggagagga
    27301 catcactaaa cgccgaaaaa ttcagcggca tatcaaataa tacgtaatcg ccgcccagta
    27361 atatcaaatc gggcttttgt tctatgccaa gagcaatcgc gtcagaaatc aggcttaaag
    27421 gaacaaaacg agagtaatgg agatcggcca gaaaaagaat tttgaatggt gctgcgttgt
    27481 ctttaaaaaa ggcgaggcgg tgacggatta attcaaacca gcctggctcc cagtaatgca
    27541 tataaccgaa acctgagctc gtggctatcg tcgcggcagt agcctgcaac aatcggcggc
    27601 gtgaaatcat cgctcatccc tgcaaaaaaa atcgggcagc gtcgtgctgc ccgtgtgcat
    27661 acttttagtc gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt
    27721 caactttctt aacgataacc gcacagtaga ggctgtattt gccatctttt gacggcagat
    27781 tacctgaaac aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac
    27841 ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat ggaaatgacg gaaccttctt
    27901 cgacaatcac cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaatga
    27961 tggttgggtt agcctgcagc ggttccagca cgccgccgat gcccacgcca ccggaaaggt
    28021 ggacgttttt accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc
    28081 cttcatcaac atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa
    28141 taaacgcacc ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac
    28201 gtgcttcgtc gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc
    28261 cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact
    28321 gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcaccg gaatccagca
    28381 gggcgatcac ctgatttacc gcttcgcggg taacggtgtc tgcgttggct ggcgtgatct
    28441 cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct
    28501 tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct
    28561 gatgcacttc ctgctgcaac tcgttattaa gtgcacgccg gtcagcggtg gcaattatga
    28621 ataaatcttc tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca
    28681 gatcggcaaa aattttcccg actctcgcca gcagtcctgg ctggtcgagc gcgataagct
    28741 caaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa
    28801 aatggcgtaa tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca
    28861 gtacttgctc cagaccaaac cgaataacct catgacgatc tgcggacagc gggctgccat
    28921 cgggttccag cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg
    28981 cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg
    29041 ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca
    29101 ataccagcgg tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat
    29161 ttgggctatg gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct
    29221 cttcgtcgat gttatccatg cgcagtagtg ccagtgcctg gagttggtga tgacgaaccc
    29281 gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat
    29341 agagttcgcg caacagactt tgcttccagc tattccacag cgtttcgttg gtggcgcaaa
    29401 tgtcagccac cgtcaggcat accagataac gaagtcggtt ttctgtttgc acttcttcgg
    29461 caaactgctt gatgacttcc gggtcctgaa tatcgcggcg ttgggcggtc accgacatca
    29521 acaggtgctg gcgaaccagc caggcaacca gctgtgtttc gcgcgagtta agcccgtgga
    29581 gttcggcaaa atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt
    29641 tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc
    29701 agacgtccac acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt
    29761 tcagcatcac gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca
    29821 tctgcccgac gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca
    29881 ttggcaatag cccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc
    29941 gtgcttccgg aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc
    30001 gcagcgtggt ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac
    30061 gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac
    30121 cgcgtagctg aaactcatcg tcgagtggac gtggtttttc gtcggcggga agggcgagga
    30181 tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc
    30241 ggaagtaatc cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac
    30301 gctgagcgac gctaagttgg cgatcgaata acagacgatt atcgtaacgg ctgacgacca
    30361 gatgcagggc aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct
    30421 ccgctgaggt taagaagcca aacccaacca tttcatccag cgatgttgcg ccaaaatgac
    30481 ggcgggccac ccattgcaga gtgtggatat cgcgcaagcc gccagggcta cttttgatgt
    30541 ctggttcaag attgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa
    30601 ctttcgccgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca
    30661 gttcgaggaa gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga
    30721 cggttaaatc cgataaccct tccagcatgc actcttcaag cgtgcgcaca ctatgaccga
    30781 cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt
    30841 ccgggagctt tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc
    30901 cacgaccgta gccaccgacg gcgaccaatg ccaggtcggc aatctggctg aatcccgctt
    30961 caatccataa tcgttgcagg agctggtcga taaactcggt gcgcgcctca atcaactgtt
    31021 ctgcagagat cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg
    31081 cttttatccc accgacggtt aattcatcac ggggccagac gcatggattt tgcggttgac
    31141 cgggcagggt ggggagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc
    31201 acccataaga ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg
    31261 tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat ttcgcagccg
    31321 ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt
    31381 accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctcg
    31441 atggtgaacg tcatcccagg tttcagtacg acgttggttt cacgggagtc atagtgcagc
    31501 acctgcggtt cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg
    31561 gagaagcctt ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg
    31621 cctggtttga ccatgcgcag cgccaggtac aggctttctt gcgtgatgcg gcacagacgt
    31681 tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca
    31741 tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag cttcgcatcg
    31801 tccgggatac cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg
    31861 tgatagccga ggcaggcaga aaccgtgtgt tgttcattaa caatgtaatc attacagatg
    31921 cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact
    31981 tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag
    32041 atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt
    32101 caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg
    32161 aacgttttct ataatagaaa attcaacgtc tgatgctgta cacagcgcca acaattattg
    32221 gtgtccatga cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac
    32281 agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt
    32341 ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat
    32401 atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac
    32461 ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt
    32521 aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct
    32581 gaactgaaca agattgcttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct
    32641 gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc
    32701 tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa
    32761 gacctggaaa ctcagtctca ggacggtact ttcgaaaagc tgaccaagaa agaagcgctg
    32821 atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc
    32881 ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa
    32941 gcaaacaacc tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt
    33001 gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc
    33061 gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa
    33121 agcttcgtag aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag
    33181 aaaggcggca agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga
    33241 gcgaagacaa cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta
    33301 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    33361 tttaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    33421 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    33481 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    33541 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    33601 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg
    33661 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    33721 ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33781 agaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33841 acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33901 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg
    33961 ctgaagtggt agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    34021 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    34081 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    34141 agcataacgc tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag
    34201 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa
    34261 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    34321 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    34381 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    34441 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    34501 tcgtatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    34561 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    34621 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ctggcaatgc gtgatgcact
    34681 gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga
    34741 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34801 tgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34861 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc
    34921 ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa
    34981 agagctgaaa gtcatggacc tagcggcctt cacgctggct cgtgaccata aattaccgat
    35041 tcgtgttttc aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    35101 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    35161 gtatcgcggg ggcgttagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg
    35221 ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc
    35281 caaaatggca aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag
    35341 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    35401 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg
    35461 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    35521 gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    35581 cgtaacggta gaagattccc gtacactgaa aatcaacgtg tttgatcgtt caatgtctcc
    35641 ggccgttgaa aaagcgatta tggcgtccga tcttggcctg aacccgaact ctgcgggtag
    35701 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    35761 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aacgtgcgtc gtgacgcgaa
    35821 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35881 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35941 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    36001 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    36061 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    36121 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    36181 ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag
    36241 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa
    36301 gtgggcaaca agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag
    36361 ccattgttgg cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    36421 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    36481 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt
    36541 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    36601 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    36661 caatgacgcc ggatcaagcc tgccgtcatc cgaactggtc gatggggcgt aaaatttctg
    36721 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36781 acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36841 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36901 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36961 gcaaactaag tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac
    37021 tggcgatgga ggcgttcgaa caaggtcagg cagcgacgac agcattgaat gccgcaaacg
    37081 aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga
    37141 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    37201 ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    37261 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    37321 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    37381 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa
    37441 acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg
    37501 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    37561 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    37621 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    37681 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    37741 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg
    37801 tttgcaagaa cgtattcgta aatctgaagc gctaacagcc gggaatagcg gtctgacgct
    37861 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37921 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca
    37981 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca
    38041 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    38101 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    38161 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    38221 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt
    38281 tctgttgccg ccggtggggt tcgccatcgt aacgctggtg gtctgcatgc tggcagcgtg
    38341 ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt
    38401 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    38461 tcaaccgctg gttgaaatct cactttgggc ttcgttgggt tggtggattg tcgcgctatt
    38521 gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    38581 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    38641 gcactacgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    38701 atggggcgct gactctggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    38761 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactag cgaccgcagc
    38821 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38881 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38941 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    39001 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    39061 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    39121 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    39181 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    39241 actgataagc tcggcaccga atatgttatc gccctgatcc cgctgggcgg ttatgtcaaa
    39301 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    39361 aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt
    39421 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    39481 ggtgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    39541 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    39601 aaaattggcg atgaaagcac caccattaca gtagcgccat ttggcagcga ccaacggcgg
    39661 gatgtaaagc tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct
    39721 tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcaacca
    39781 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39841 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39901 ttagcgttag aaatcgaaag gcagggaagt cccttgtctt tgacattaat cccggagagt
    39961 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    40021 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    40081 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    40141 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    40201 gcggaactcg gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    40261 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    40321 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    40381 tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    40441 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    40501 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    40561 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag
    40621 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    40681 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    40741 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40801 agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40861 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40921 taaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg
    40981 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    41041 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg
    41101 atcgtaaata ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agctactatc
    41161 tggatcgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata
    41221 aaaaaggtat ttacgtcacg gtgaacatta ccgaaggcga tcagtacaag ctttctggcg
    41281 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg
    41341 agccgggcga gctgtataac ggcaccaaag tgaccaagat ggaagatgac atcaaaaagc
    41401 ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg
    41461 ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    41521 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    41581 agatggaagg tgcatggctg ggaagcgatc tggtcgatca gggtaaggag cgtctgaatc
    41641 gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc
    41701 aggtagatgt cgtctacaag gtaaaagagc gcaacaccgg tagcttcaac ttcgggattg
    41761 gttacggtac tgaaagtggc gtgagcttcc aggctggtgt gcagcaggat aactggttag
    41821 gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41881 tgtcggtaac caacccgtac ttcactgtag atggtgtaag cctcggtggt cgtctcttct
    41941 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    42001 cagacgtgac gttgggcttc ccgatcaacg aatataactc gctgcgtgca ggtctgggtt
    42061 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    42121 ctatgggtga acatccgagc acctctgatc aggataatag cttcaaaacg gacgacttca
    42181 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    42241 cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag
    42301 tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg
    42361 ggcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga
    42421 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaataca attggtccga
    42481 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    42541 cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca
    42601 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    42661 actcggttcg tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt
    42721 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42781 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42841 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42901 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42961 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    43021 attgtgaaaa agtggttatt agcggcaggt ctcggtttag cactggcaac ttctgctcag
    43081 gcggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt agcgcagaaa
    43141 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    43201 atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat
    43261 cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
    43321 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    43381 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    43441 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    43501 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    43561 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    43621 ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    43681 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    43741 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43801 gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta
    43861 tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc
    43921 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43981 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    44041 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    44101 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat
    44161 cgcggcgcgc tggatgacac tattattggc aatggcgtga tcattgataa ccagtgccag
    44221 attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg
    44281 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    44341 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    44401 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    44461 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    44521 gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt
    44581 cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta
    44641 tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc
    44701 gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg
    44761 cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga
    44821 ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta
    44881 aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgctggtatt gacgaagcgc
    44941 gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa
    45001 cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgatggtaaa gtagtttgcg
    45061 aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct
    45121 ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcaat tggcgcgaac gcacacattg
    45181 gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc
    45241 acgttgtcgt gaatggtcat actaaaattg gccgcgataa tgagatttat cagttcgcct
    45301 ccatcggcga agttaaccag gatctgaaat atgctggcga accgacccgt gtggaaatcg
    45361 gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg
    45421 gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt
    45481 gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg
    45541 ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg
    45601 cgcacgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct tatgtcattg
    45661 cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat
    45721 tcagccgtga ggcgattacc gctatccgca atgcgtataa gctgatttat cgtagcggta
    45781 aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat ccggaagtga
    45841 aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgaaca
    45901 gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg
    45961 tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggtg ttgccgggcc
    46021 acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg
    46081 cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa
    46141 gcgttttggc gaactgaagc cagatatttt tgttggtatt gatgcgcctg acttcaatat
    46201 tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc attcattacg tcagtccatc
    46261 cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct
    46321 cgcatttctg cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat
    46381 cggtcatacc atggctgatg ccatgccgtt agatccagat aaaaatgccg cccgtgatgt
    46441 gctggggatc cctcacgatg cccactgcct ggcgttgcta ccagggagcc gtggtgcaga
    46501 agttgaaatg cttagtgccg atttcctgaa aacggcccag cttttgcgcc agacatatcc
    46561 ggatctcgaa atcgtggtgc cactggtgaa tgccaaacgc cgcgagcagt ttgaacgcat
    46621 caaagctgaa gtcgcgccag acctttcagt tcatttgctg gatgggatgg gccgtgaggc
    46681 gatggtcgcc agcgatgcgg cgctactggc gtcgggtacg gcagccctgg agtgtatgct
    46741 ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa
    46801 gcggctggtg aaaactgatt atgtctcgct gccaaatctg ctggcgggta gagagttagt
    46861 caaagaatta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct
    46921 gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca
    46981 gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc
    47041 gaatttgttt atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg
    47101 ttagttggcg cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg
    47161 ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa
    47221 gagaaagcgt tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac
    47281 attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg
    47341 gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg
    47401 gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt ctatcctggc gaaagtgacg
    47461 cgtgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacac
    47521 aaagggtacc caaccgcttt tcatctggaa aaactggctg aacacggcgc gaccgaacac
    47581 catcggcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt
    47641 cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg
    47701 cacagcgact actcgatgat cgatggcctg gccaaaaccg caccgttggt aaaaaaggcg
    47761 gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg tggtctggtg
    47821 aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaac
    47881 gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat
    47941 accggctatc agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc
    48001 gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt
    48061 tccggcggac gcatgggcga cgtcggacgc agtcttttgc gtggtaacag agcgctggta
    48121 gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc
    48181 cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg
    48241 cgcggtttgc ccgtcgtggc gaccaacgac gtgcgcttta tcgacagcag cgactttgac
    48301 gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg
    48361 cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc
    48421 gacatccccg aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaaccgtg
    48481 cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat
    48541 ctggtcaagc gtgcaaaaga gggcctggaa gagcgtctgg cctttttatt ccctgacgag
    48601 gaagaacgtg ttaagcgccg cccggaatat gacgaacgtc tggagactga acttcaggtt
    48661 atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg
    48721 aaagataacg gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc
    48781 tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc
    48841 cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc
    48901 gatcaggtta tcgagcacgt agcggacatg tacggtcgtg atgcggtatc gcagatcatc
    48961 accttcggta caatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat
    49021 ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg
    49081 gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt
    49141 aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac
    49201 gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttactgcgat
    49261 gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg
    49321 gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg
    49381 atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg atatcgctgc gatcccgctg
    49441 gatgataaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt
    49501 gaatcgcgcg gcatgaagga cctgatcaag cgtctacaac ctgactgctt cgaagatatg
    49561 atcgccctag tggcactgtt ccgccccggt ccgttgcaat cagggatggt ggataacttt
    49621 atcgaccgta aacatggtcg tgaagagatc tcctatccgg acgtacagtg gcagcatgaa
    49681 agcctgaaac cggtactgga gccaacctac ggcattatcc tgtatcagga acaggtcatg
    49741 cagattgcgc aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgtcgtgcg
    49801 atgggtaaga aaaagccgga agagatggct aagcaacgtt ctgtatttgc tgaaggtgca
    49861 gaaaagaacg gaatcaacgc tgaactggcg atgaaaatct tcgacctggt ggagaaattc
    49921 gctggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg
    49981 ttatggctga aagcacacta tcctgcggag tttatggcgg cggtaatgac cgccgatatg
    50041 gacaacaccg agaaggtggt gggtctggtg gatgagtgct ggcggatggg gctgaaaatc
    50101 ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc
    50161 gtgtatggta ttggcgcgat caaaggggtc ggtgaaggtc cgattgaggc catcatcgaa
    50221 gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg taccgacacc
    50281 aaaaagttga accgtcgcgt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt
    50341 gggccacacc gcgcggcgct gatgaactcg ctgggtgatg cgttaaaagc ggcagatcaa
    50401 cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg
    50461 gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat
    50521 ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa
    50581 gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt
    50641 aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc
    50701 aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt
    50761 actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc
    50821 ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg
    50881 gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg
    50941 caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg
    51001 acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc
    51061 gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt
    51121 tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt
    51181 gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc
    51241 cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa
    51301 agcgtagaac tgacgcgtaa aatcttcgcc gatcttggtg catggcagat tgcgcaactg
    51361 gcacgccatc cacagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt
    51421 gacgaactgg ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc
    51481 cgtcttgacg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa
    51541 aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg
    51601 atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct
    51661 tatccgggcg tgggcgcaga agaacgtggt cagtctgaag ccattgcacg caacctgcgt
    51721 gaaatgtctc gcctcggtgt accggtagtt tgtacggtta tcggtgaagg tggttctggc
    51781 ggcgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc
    51841 gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgctgacaa agcgccgctg
    51901 gcagctgaag cgatgggtat catcgctccg cgtctgaaag aactaaaact gatcgactcc
    51961 atcatcccgg aaccgctggg tggtgctcac cgtaacccgg aagcgatggc ggcatcgttg
    52021 aaagcgcaac tgctggcgga tctggccgat ctcgacgtgt taagcactga agatttaaaa
    52081 aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa
    52141 aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa
    52201 ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt
    52261 ataaagatga gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta
    52321 tctggccaca aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg
    52381 gcgtgatttt tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta
    52441 atgaatatct cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc
    52501 aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg
    52561 ccattcgtat gcgtcagtac accgacgaat atcttgataa cattacaccg ccgttcacga
    52621 aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg
    52681 gcggcaccgc atatcaaaaa agcccggttg gctgcctgtt ttatgatttt ttcggcggga
    52741 ataccctcaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg ctcgaccaca
    52801 ccgggccaca cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt
    52861 atatcgttac caacggaaca tcgacgtcga acaaaattgt gggtatgtac gccgcgccat
    52921 ccggcagtac gctgttgatc gaccgcaatt gtcataaatc gctggcgcat ctgttgatga
    52981 tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attcttggtg
    53041 ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccaccacgc
    53101 aggcacaatg gccggttcat gcggtgatta ccaactccac ctatgatggc ttgctctaca
    53161 acaccgactg gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg
    53221 tgccgtacac ccattttcat ccgatctacc aggggaaaag tggtatgagc ggcgagcgtg
    53281 ttgcgggaaa agtgatcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc
    53341 aggcttcgct gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta
    53401 tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttgag acggcggcgg
    53461 cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc
    53521 attttcgcaa agaggtccag cggttgcggg aagagtctga cggttggttt ttcgatatct
    53581 ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt tgcgctaggc gaacagtggc
    53641 acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt
    53701 tgacaccggg gatggacgaa cagggcaata tgagcgagga ggggatcccg gcggcgctgg
    53761 tggcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc
    53821 tgtttctctt tagtattggt atcgataaaa ccaaagcaat gggattattg cgtgggttga
    53881 cggaatttaa acgctcttac gatctcaacc tgcggatcaa aaatatgcta cccgatctct
    53941 atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc
    54001 ataagctgat tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc gatactttgc
    54061 cggaaatgat catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa
    54121 ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac
    54181 cgggcgtacc gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg
    54241 attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc
    54301 acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat
    54361 aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat
    54421 gctgggttta aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa
    54481 agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg
    54541 cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt
    54601 tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt
    54661 tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga
    54721 agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg
    54781 gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt
    54841 gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc
    54901 tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc
    54961 atcagttggt gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc
    55021 atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac
    55081 agtggcaggt gccgctggtg gtcgaacgcg tgcaacttgc gcaagaagga ctgggcattg
    55141 aggcccaggc gcggcaggcg cgttatcagg catttgcccg caccttgttg cccggtgaag
    55201 tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ttttctgctg gcgctaaaac
    55261 gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt gccggaacac
    55321 ggcttatccg cccgttgctc gcccgcacgc ggggagaact ggagcagtgg gcattggcgc
    55381 atggtttacg ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc
    55441 tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcg gaagcaacgg
    55501 cccgcagcgc cgcactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg
    55561 atttagcaca ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctggcgatga
    55621 gtgatgcccg ccgcgcggcg attatccgtc gctggctggc agggcagaat gcaccaatgc
    55681 cttcccgcga cgcgctggtg cggatctggc aggaagtggc gctggcgcgg gaagatgcct
    55741 ccccctgttt acgcttgggc gcgtttgaaa tccgccgcta tcagtcgcaa ctgtggtgga
    55801 ttaaatccgt caccgggcaa agtgaaacca ttgtgccgtg gcagacgtgg ctacaaccgc
    55861 tggaattacc ggcggggctg ggaagcgtac agcttactgc gggaggcgat attcgccctc
    55921 cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcggc aggattgctg catattgtcg
    55981 ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga gttaggcgtg ccgccgtggc
    56041 tacgtgacac cacgccactg ctgttttatg gcgaaacgct gattgcggcg gcaggggtat
    56101 ttgtgacgca agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa
    56161 cgcttagtta agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat
    56221 caggactcgc tcaccaccac cgtaccgatt tccgggtggc taaagctggt aattttatcc
    56281 agacgtagct cacgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg
    56341 gagactaaat cgctggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc
    56401 attaaatgat gctggcaggc gagctcaaga ttatcgtaat catcacaatt gattggttga
    56461 tacgtatcat tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt
    56521 ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac
    56581 gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt
    56641 gcgtattaac tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg
    56701 gatgcaagtc taagcctacg aagataaact ctgtttcgca aggtgactat accacactca
    56761 tgtctgcaat atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa
    56821 aggagttttc ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat
    56881 ctcgaccgca accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc
    56941 caggagcgta tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa
    57001 tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg
    57061 ggcattgatt tgtggattga gctggggctg ccggatgagc ggcggattaa gaaagcctgc
    57121 acccaggccg cagaagtggc gctgtttacc tataatagtc gggcggcgca aatctggtgg
    57181 cagcaaaatc agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat
    57241 gaacaactgg cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt
    57301 caggatggcg tgatctggtt atcggacgat aagaataatc tggaagttaa cttaaccgcc
    57361 tggcaacaac cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg
    57421 agatcaccgc cattcgtgcg cagggcgcgg gcgggcagca tgttaataag acctcaacgg
    57481 ccattcatct gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc
    57541 tactcgccgc cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg
    57601 aataccgcag tcaggaactg aaccgcgaag ccgcgctagc ccggctggtg gcagtgatta
    57661 aagatttaac aacagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag
    57721 agcgcaggct ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac
    57781 gcagcggtcg ggaataaaaa gaaggaatag atggtgaaaa aagcgatagt gacagcgatg
    57841 gctgtaatca gcctctttac tctgatggga tgtaataacc gggccgaagt cgatacgctt
    57901 tctccagcac aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctgccg
    57961 tgtgctgatt gcgaaggaat cgaaacctct ctgttcctcg aaaaagacgg aacatgggtg
    58021 atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca
    58081 tgggcgcgaa ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat
    58141 cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag gcaatccgat tgaatcgcag
    58201 ttcaactata cgctggaacc ggcacaatct agtttaccta tgacgccgat gaccctgcga
    58261 ggcatgtatt tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt
    58321 ttcatggtag cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt
    58381 gaaaaaccgg tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact
    58441 ggtgcgccaa ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat
    58501 tgcagtagtt tggggcagta aacctgctta aggcagaaac aaacgcaa
//
LOCUS       DAZFRJ010000007        57953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000007 DAZFRJ010000000
VERSION     DAZFRJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.007"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..1336
                     /gene="preT"
                     /locus_tag="ACHKUN_000382"
     CDS             98..1336
                     /gene="preT"
                     /locus_tag="ACHKUN_000382"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO6658705.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            1330..2565
                     /gene="preA"
                     /locus_tag="ACHKUN_000383"
     CDS             1330..2565
                     /gene="preA"
                     /locus_tag="ACHKUN_000383"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO6658706.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(2636..3646)
                     /gene="mglC"
                     /locus_tag="ACHKUN_000384"
     CDS             complement(2636..3646)
                     /gene="mglC"
                     /locus_tag="ACHKUN_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO6658707.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(3662..5182)
                     /gene="mglA"
                     /locus_tag="ACHKUN_000385"
     CDS             complement(3662..5182)
                     /gene="mglA"
                     /locus_tag="ACHKUN_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO6658708.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(5243..6241)
                     /gene="mglB"
                     /locus_tag="ACHKUN_000386"
     CDS             complement(5243..6241)
                     /gene="mglB"
                     /locus_tag="ACHKUN_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO6658709.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(6521..7561)
                     /gene="galS"
                     /locus_tag="ACHKUN_000387"
     CDS             complement(6521..7561)
                     /gene="galS"
                     /locus_tag="ACHKUN_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO6658710.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(7703..8860)
                     /gene="yeiB"
                     /locus_tag="ACHKUN_000388"
     CDS             complement(7703..8860)
                     /gene="yeiB"
                     /locus_tag="ACHKUN_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO6658711.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(8877..9545)
                     /gene="folE"
                     /locus_tag="ACHKUN_000389"
     CDS             complement(8877..9545)
                     /gene="folE"
                     /locus_tag="ACHKUN_000389"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO6658712.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            9803..10639
                     /gene="yeiG"
                     /locus_tag="ACHKUN_000390"
     CDS             9803..10639
                     /gene="yeiG"
                     /locus_tag="ACHKUN_000390"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6658713.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(10671..12662)
                     /gene="cirA"
                     /locus_tag="ACHKUN_000391"
     CDS             complement(10671..12662)
                     /gene="cirA"
                     /locus_tag="ACHKUN_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO6658714.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(12955..14424)
                     /gene="lysP"
                     /locus_tag="ACHKUN_000392"
     CDS             complement(12955..14424)
                     /gene="lysP"
                     /locus_tag="ACHKUN_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO6658715.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(14629..15510)
                     /gene="yieE"
                     /locus_tag="ACHKUN_000393"
     CDS             complement(14629..15510)
                     /gene="yieE"
                     /locus_tag="ACHKUN_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO6658716.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15609..16658
                     /gene="yeiH"
                     /locus_tag="ACHKUN_000394"
     CDS             15609..16658
                     /gene="yeiH"
                     /locus_tag="ACHKUN_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO6658717.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            16732..17589
                     /gene="nfo"
                     /locus_tag="ACHKUN_000395"
     CDS             16732..17589
                     /gene="nfo"
                     /locus_tag="ACHKUN_000395"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO6658718.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            17593..18681
                     /locus_tag="ACHKUN_000396"
     CDS             17593..18681
                     /locus_tag="ACHKUN_000396"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6658719.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(18737..19987)
                     /gene="nupX"
                     /locus_tag="ACHKUN_000397"
     CDS             complement(18737..19987)
                     /gene="nupX"
                     /locus_tag="ACHKUN_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6658720.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(20087..21028)
                     /gene="rihB"
                     /locus_tag="ACHKUN_000398"
     CDS             complement(20087..21028)
                     /gene="rihB"
                     /locus_tag="ACHKUN_000398"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO6658721.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            21158..21856
                     /gene="yeiL"
                     /locus_tag="ACHKUN_000399"
     CDS             21158..21856
                     /gene="yeiL"
                     /locus_tag="ACHKUN_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO6658722.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(21924..23174)
                     /locus_tag="ACHKUN_000400"
     CDS             complement(21924..23174)
                     /locus_tag="ACHKUN_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6658723.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(23268..24206)
                     /gene="psuG"
                     /locus_tag="ACHKUN_000401"
     CDS             complement(23268..24206)
                     /gene="psuG"
                     /locus_tag="ACHKUN_000401"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO6658724.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(24194..25135)
                     /gene="psuK"
                     /locus_tag="ACHKUN_000402"
     CDS             complement(24194..25135)
                     /gene="psuK"
                     /locus_tag="ACHKUN_000402"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO6658725.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(25559..27250)
                     /gene="fruA"
                     /locus_tag="ACHKUN_000403"
     CDS             complement(25559..27250)
                     /gene="fruA"
                     /locus_tag="ACHKUN_000403"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO6658726.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(27267..28205)
                     /gene="fruK"
                     /locus_tag="ACHKUN_000404"
     CDS             complement(27267..28205)
                     /gene="fruK"
                     /locus_tag="ACHKUN_000404"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO6658727.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(28205..29335)
                     /gene="fruB"
                     /locus_tag="ACHKUN_000405"
     CDS             complement(28205..29335)
                     /gene="fruB"
                     /locus_tag="ACHKUN_000405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO6658728.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            29703..30884
                     /gene="setB"
                     /locus_tag="ACHKUN_000406"
     CDS             29703..30884
                     /gene="setB"
                     /locus_tag="ACHKUN_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO6658729.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(30881..31135)
                     /gene="yeiW"
                     /locus_tag="ACHKUN_000407"
     CDS             complement(30881..31135)
                     /gene="yeiW"
                     /locus_tag="ACHKUN_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6658730.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            31290..31862
                     /gene="yeiP"
                     /locus_tag="ACHKUN_000408"
     CDS             31290..31862
                     /gene="yeiP"
                     /locus_tag="ACHKUN_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO6658731.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            32085..33551
                     /gene="yeiQ"
                     /locus_tag="ACHKUN_000409"
     CDS             32085..33551
                     /gene="yeiQ"
                     /locus_tag="ACHKUN_000409"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6658732.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            33669..34655
                     /gene="yeiR"
                     /locus_tag="ACHKUN_000410"
     CDS             33669..34655
                     /gene="yeiR"
                     /locus_tag="ACHKUN_000410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO6658733.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            34694..35407
                     /gene="lpxT"
                     /locus_tag="ACHKUN_000411"
     CDS             34694..35407
                     /gene="lpxT"
                     /locus_tag="ACHKUN_000411"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO6658734.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            35819..36385
                     /gene="mepS"
                     /locus_tag="ACHKUN_000412"
     CDS             35819..36385
                     /gene="mepS"
                     /locus_tag="ACHKUN_000412"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO6658735.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            36566..38122
                     /gene="pdeN"
                     /locus_tag="ACHKUN_000413"
     CDS             36566..38122
                     /gene="pdeN"
                     /locus_tag="ACHKUN_000413"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6658736.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            38204..40018
                     /gene="yejA"
                     /locus_tag="ACHKUN_000414"
     CDS             38204..40018
                     /gene="yejA"
                     /locus_tag="ACHKUN_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO6658737.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            40019..41113
                     /gene="yejB"
                     /locus_tag="ACHKUN_000415"
     CDS             40019..41113
                     /gene="yejB"
                     /locus_tag="ACHKUN_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO6658738.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            41113..42138
                     /gene="yejE"
                     /locus_tag="ACHKUN_000416"
     CDS             41113..42138
                     /gene="yejE"
                     /locus_tag="ACHKUN_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6658739.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            42140..43729
                     /gene="yejF"
                     /locus_tag="ACHKUN_000417"
     CDS             42140..43729
                     /gene="yejF"
                     /locus_tag="ACHKUN_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO6658740.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(43733..44077)
                     /gene="yejG"
                     /locus_tag="ACHKUN_000418"
     CDS             complement(43733..44077)
                     /gene="yejG"
                     /locus_tag="ACHKUN_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO6658741.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(44410..45600)
                     /gene="bcr"
                     /locus_tag="ACHKUN_000419"
     CDS             complement(44410..45600)
                     /gene="bcr"
                     /locus_tag="ACHKUN_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO6658742.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(45628..46323)
                     /gene="rsuA"
                     /locus_tag="ACHKUN_000420"
     CDS             complement(45628..46323)
                     /gene="rsuA"
                     /locus_tag="ACHKUN_000420"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO6658743.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            46472..48232
                     /gene="radD"
                     /locus_tag="ACHKUN_000421"
     CDS             46472..48232
                     /gene="radD"
                     /locus_tag="ACHKUN_000421"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6658744.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGNEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            48357..48641
                     /gene="rplY"
                     /locus_tag="ACHKUN_000422"
     CDS             48357..48641
                     /gene="rplY"
                     /locus_tag="ACHKUN_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO6658745.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(48780..49787)
                     /gene="yejK"
                     /locus_tag="ACHKUN_000423"
     CDS             complement(48780..49787)
                     /gene="yejK"
                     /locus_tag="ACHKUN_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO6658746.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            49969..50196
                     /gene="yejL"
                     /locus_tag="ACHKUN_000424"
     CDS             49969..50196
                     /gene="yejL"
                     /locus_tag="ACHKUN_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO6658747.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            50216..51976
                     /gene="yejM"
                     /locus_tag="ACHKUN_000425"
     CDS             50216..51976
                     /gene="yejM"
                     /locus_tag="ACHKUN_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO6658748.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            52051..52127
                     /locus_tag="ACHKUN_000426"
     tRNA            52051..52127
                     /locus_tag="ACHKUN_000426"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52085..52087,aa:Pro,seq:ggg)
     gene            52346..53587
                     /locus_tag="ACHKUN_000427"
     CDS             52346..53587
                     /locus_tag="ACHKUN_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO6658749.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            53686..53916
                     /locus_tag="ACHKUN_000428"
     CDS             53686..53916
                     /locus_tag="ACHKUN_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658750.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            54084..54290
                     /locus_tag="ACHKUN_000429"
     CDS             54084..54290
                     /locus_tag="ACHKUN_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6658751.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            54305..54781
                     /locus_tag="ACHKUN_000430"
                     /pseudo
     CDS             54305..54781
                     /locus_tag="ACHKUN_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            54974..55312
                     /locus_tag="ACHKUN_000431"
                     /pseudo
     CDS             54974..55312
                     /locus_tag="ACHKUN_000431"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            55343..55534
                     /locus_tag="ACHKUN_000432"
     CDS             55343..55534
                     /locus_tag="ACHKUN_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6658752.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            55524..55745
                     /locus_tag="ACHKUN_000433"
     CDS             55524..55745
                     /locus_tag="ACHKUN_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658753.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            55738..55959
                     /locus_tag="ACHKUN_000434"
     CDS             55738..55959
                     /locus_tag="ACHKUN_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658754.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEAVLRKTTDGD"
     gene            55961..56194
                     /locus_tag="ACHKUN_000435"
     CDS             55961..56194
                     /locus_tag="ACHKUN_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6658755.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            56200..56499
                     /locus_tag="ACHKUN_000436"
     CDS             56200..56499
                     /locus_tag="ACHKUN_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6658756.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            56496..57896
                     /locus_tag="ACHKUN_000437"
     CDS             56496..57896
                     /locus_tag="ACHKUN_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO6658757.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSQKRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDKLKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPV
                     ELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKL
                     ENDGVIVIDGDDVRPL"
BASE COUNT        14472 a        14976 c        14399 g        14106 t
ORIGIN      
        1 ctggggtgag cgaacgcaga cgcagcacat gcaacttgaa gtatgacgag tatatttagc
       61 gttcgcctta attactcata gcattaagga agatcacatg ccgcaacaaa attatctgga
      121 tgaactcact ccggctttta cgcctttact ggcgattaaa gaagcctctc gctgtttatt
      181 atgtcatgac gctccctgta gtcaggcttg cccggcacag accgatccgg ggaaatttat
      241 tcgctcaatc tactttcgta attttaaagg cgctgccgag acaattcgcg aaaataatgc
      301 cctcggtgcc gtttgtgcca gagtgtgccc gacggagaaa ttatgtcaaa gcggttgtac
      361 ccgtgccggt gttgatactc ccattgatat cggtcgctta cagcgttttg ttactgattt
      421 tgaacaacaa accgggatgg aaatttatca gcccggtact aaaacgctcg gcaaagtcgc
      481 gattattggc gcaggtcctg ccggattgca ggccagtgta acactgacaa accagggtta
      541 tgacgtcacg atttatgaga aagaagcgca ccccggtggc tggttgcgta acggtattcc
      601 gcaattccgg ttaccgcaat cagtgctgga tgcagagatc gcccgtattg aaaaaatggg
      661 cgtgaccatt aagtgcaaca acgaagtcgg taacacactc acccttgagc agctgaaagc
      721 agaaaaccgc gcggtactgg tcaccgtggg gttatcgagc ggttccgggt tatcgctgtt
      781 tgagcatagc gacgttgaga ttgccgtcga cttcctgcaa cgtgcacgac aggcgcaagg
      841 cgatatcagc attccacaaa gcgcattaat tatcggcggc ggtgatgttg cgatggacgt
      901 agccagcacg ctgaaagttc tcggctgtca ggcggtaacc tgcgtagcgc gtgaagagtt
      961 agatgagttt ccggcaagcg aaaaagagtt tgccagcgcc cgggagctgg gcgtttcgat
     1021 catcgatgga ttcacgccag tagccgtgga aggcaataaa gtcacgttta agcatgtacg
     1081 gttaccgggc gaactgacga tggcggcaga taaaattatt ctcgccgtcg gtcagcatgc
     1141 cagactggat gcctttgcgg agttagagcc gcagcgtaac accatcaaaa cacaaaatta
     1201 tcagacccgc gatccgcaag tctttgctgc tggcgatatt gttgagggtg acaaaaccgt
     1261 ggtctatgcc gtgaaaaccg ggaaagaagc cgccgaggcg attcatcact atttagaggg
     1321 agcttgctca tgttaacgaa agatctttcg attacttttt gcggcgtgaa gtttcccaac
     1381 ccgttctgcc tctcttcttc gcctgtaggc aactgctatg agatgtgtgc caaagcctac
     1441 gacacaggtt ggggcggcgt ggtgtttaaa acgatcggct tttttatcgc caacgaagtc
     1501 tcgccgcgtt ttgatcatct ggtgaaagaa gataccggtt ttatcggctt caaaaatatg
     1561 gagcagattg ctgaacatcc gttggaagag aatctggccg ccctgcgtca gctgaaggaa
     1621 gattacccgg acaaagtatt gatcgcttcg atcatggggg aaaatgagca gcaatgggag
     1681 gagctggcgc gcctggtgca agaagctggc gcggatatga tcgagtgtaa cttctcctgt
     1741 ccgcaaatga cttctcatgc gatgggtagc gatgtcgggc aaagcccgga gctggtagaa
     1801 aaatattgtc gggcagtgaa acggggttcc acgctgccaa tgctggcgaa gatgacgccg
     1861 aatatcggtg atatgtgcga agtggcgctg gcggcgaagc gcggcggcgc agatggcatt
     1921 gcggcgatta acaccgttaa atccatcacc aatatcgatc ttaatcagaa aatcggtatg
     1981 ccgatcgtta acggaaaatc gagtatttcc ggatattccg gtaaagcggt aaaaccgatc
     2041 gccctgcgct tcattcagca gatgcgcacc catccagaac tgcgcgattt cccaatcagc
     2101 ggtatcggcg gcattgaaac ctgggaggat gcggctgagt ttttattgct cggcgcagca
     2161 acgttacagg tgaccaccgg catcatgcag tacgggtatc ggatagtgga agatatggca
     2221 agcgggttgt cgcattatct cgccgatcag ggatttgatt cgctgcagga gatggtaggt
     2281 ctggcgaata acaatattgt cccggcggaa gatttagacc gcagttatat tgtctatccc
     2341 cgtatcaatc ttgataaatg tgttggctgt ggacgctgtt atatttcctg ttacgacggc
     2401 ggtcaccagg cgatggaatg gagcgagaaa acccgcacac cgcattgtaa taccgagaaa
     2461 tgtgtgggtt gtctgctttg tggtcacgtc tgcccggtgg gttgtattga gctcggggaa
     2521 gtgaagttta agaaaggcga gaaagaacac ccggtaacgt tgtaaaacca gagacgcatc
     2581 cggcatttgg caccgattgc cggatgcagc gcatccggca atatccacaa tcacatcact
     2641 tcttacgcgc gtatttcagt gaatccagcg ctacggcgaa gataataatc gcccctttga
     2701 tgatgtactg ccagtatggg tttacgccga tatacgtcag gccatagttg atgacggtaa
     2761 aaataattac cccggtcacc acgccaatca ccgtccccac accgccgctg aacgatacac
     2821 cgcctaccac gcacgccgcg atagcatcca gctcatacat aaagccgagg ttgttggtgg
     2881 cagagccgat acgtccggct tctaacatcc cgccaaaggc atagaacacg ccagacaacg
     2941 cgtagatcat cagcaggttc aggccgacgt tgacaccaga tacttttgcc gcttccgggt
     3001 taccgccaat ggcaaaaatg ttcttaccga agcgggtttt gttccacaac acccagacga
     3061 acgccaccgc aatcaacgcg tagaaggtga tgtaagagag acggaaactc cccagcgcga
     3121 caaagccctg agcaaaggta gagaagccac tgtcaaaacc agaaattggc gacgccccga
     3181 caaagtcgta atagagcgag ttgattccat agacgatgat catcgtgccg agcgtggtaa
     3241 tgaacggcgt cacgttgaga taagcgataa tcagaccgtt gatcaaaccg atcaccgcac
     3301 caatggcaca gacaatcaga ataaccagcg caatcggcat cgtcgccatt tccgggaaca
     3361 ctttgttggc gttatccatg gactgcaata atgtcgcagc caccactgcc gccagcccta
     3421 cctgacgacc agcagaaaga tcggtcccct gggtgacaat taaccctgcc acaccgagcg
     3481 cgataataat acgcaccgat gactgggtga gaatattact taagttcaac agacttaaaa
     3541 atgttgggtc ctggaaaata ataatcgcca gcaaaactaa aagaacgacg taaataccgc
     3601 cctctttcag gtaagtaaga aaacttttct tatttaacgc actcatggga agcccctaat
     3661 cttaaaggtg caaagacgca agacgcagaa tttcgttttg cgttgttgtt tttgtatcga
     3721 caattccgga aacgagacca ttgctcatga ccagaatacg gtctgttatc cctaacaact
     3781 caggcatttc agaggagata ataataatcc ccttgccttt cttcgccagt tcggcaatta
     3841 actgataaat ttcaaacttc gccccgacat caataccgcg cgtcggttca tcgagcatta
     3901 atatttctgg ttgcgttagt agccagcgac caataatcac cttttgctga ttaccaccgg
     3961 agagcgaacc aatttgcgtc cgatgacccg gcgtttttac ccgcatcgaa tcaatcaccc
     4021 actgggtatc gcttttcatc cgcgagttat ccagtaaacc aactttattt ttgtagttgc
     4081 gaatattgga aattaacgag ttaaaactaa tatccagata ggcataaatt cccgttgagc
     4141 ggcgctcctc agttaccagt gcaaatccgt ggtttatggc ttcgttggca ttatggttat
     4201 tgatctgttt gccgtgcaac gtaatggtgc cagccgattt ctcgcgaata ccaaataacg
     4261 tctcaacaat atcggtacgt ttcgccccca ccagcccggc aataccgagg atctcccctt
     4321 tatgcagatc aaacgagaca tcgcgaatcg acggctggcg cagtgacgtt aggttacgca
     4381 cctcgaggat gacttcgccc ggcttgtttt ctttgtcagg aaaacgctga ttaagagaac
     4441 gcccaaccat catggcgatg atcttgtcca tcgtcagtcc tgccagcggt tcggtggcga
     4501 tccactgacc gtcgcgcaat acggtaactt catcacataa ctggaagatt tcttccattt
     4561 tatgcgagat ataaacaata ccgcagccgc gctcttttaa tttacgaata atagtgaaca
     4621 gatgattgac ctctttttcg gttaacgaag aagtcggttc atccataatc acaattttcg
     4681 cgttatagga aaacgctttg gcgatttcga tcatctgcat ttgcgaaacg gataatgtgc
     4741 cgacgcgcgc acgcggatcg atatcaatat ccagttcatc aaaaatcgct ttggtttcgc
     4801 ggtacatttt gtcctgatcg acaaacatgc ctttggtggg atatcgcccc agccacatgt
     4861 tatccatcac cgaacgttgt aataccaggt ttaactcctg gtgtaccatc gaaataccat
     4921 tttccagtgc ctctttggcg gaatggaaat cgatctcttt accctggaat aaaatggtgc
     4981 cggagtcttt ttgataaata ccaaacaggc attttaataa tgtcgatttt cctgcaccgt
     5041 tttcccccat taatgcatgg atagaatgtg gccggacttt taaattaacg ttatcaagtg
     5101 ccttaacacc aggaaaggac ttgttgatac cgctcatttc caacaagtat tccccggaag
     5161 acggagtcgt tgagctgacc atataatttt accttgttgg ccatacaata agggcgcagt
     5221 aaacgactgc gcccaatcag tcttatttct tgctgaattc agccaggttg tctttatcta
     5281 cgccaacata aggtacgcgg accactttgt tgtcgatttt ccagttggtg ccatcagccg
     5341 cacctttgcc atcggccagg tttttcgcca gatcaaaggt cgctttcgcc tggttgttag
     5401 catcgttcag tactgtgccc gccagtgcac cggatttcac cagcgccagc gcttctggca
     5461 gcgcatcgac gccaaacacc ggaatgctgg acttgttgtg tgctttcagt gcttctaccg
     5521 cgcccattgc catcgcatcg ttgttggcga taaccacttc gattttgttg gcgttcgggc
     5581 cagacagcca ggcgtccatc ttatctttcg cctgagcggt atcccacata gcggtatcta
     5641 actgtaactg ttcagtttta atgcctttgt cgttcagctc tttaatcacg taagtggtac
     5701 gtgcttctgc atccggatgg cccggttcac ctttcagcaa tacgaattga atctgaccgt
     5761 ctttgttcag atcccaaccc tgattcgccg cccagtgttt agcaatcaaa tcgccctgaa
     5821 taatgccgga ctctttggaa tcagtgccaa cgtagtaggc tttgtcgtag ctatccagcg
     5881 ccttacgaga cggttctttg ttgaagaaaa ccaccggcac gttttgccca cgcgctttct
     5941 caatcaccgt acccgcagct gccgggtcaa ccaggttgat tgccagtgcc ttcacccctt
     6001 tcgccagcaa tacgtcgatc tgatcgttct gcttggactg gtcattctga gaatcattca
     6061 tcagcagctg aacatctggc gcggctttcg catcttgctc aatagccttg cgcactacag
     6121 acataaagtt atcgtcgtac ttatagattg ttacaccaat gcgagtatca gcagcgtgtg
     6181 cagcggcacc gaataacatg ctggccatca cagcagacag ggttaacacc ttcttattca
     6241 tggtatctcc ggtttttctt atgcagggta gtgcttgaga taaatgctcg gcggggcagt
     6301 agagttaatg aagtgttact gaacgccgaa gctcactttt taaaattcgt tcttccatgc
     6361 ccggtaacgc tccagaaaac ggctttaatt gttgttatga cgctattacc tcggtgaaag
     6421 tgattattca ctgttacata cgggttacaa cgttaaaacg gtgcaatcat agctatcaca
     6481 ttgttaagat actgtgaaat cactcacaga ttgaaagcgg ttacatcgcc tgatttgttg
     6541 agttagtgat cgtcgccgca ttctggcgtg ttgcgacaga atggcgacgc actaacgtcg
     6601 gcataaaaca gtggctagca cgaggatcaa tattgcctgc tgccccctgc aaggccagtt
     6661 cggtggctaa tttcgccatt gaagcaatgg gataacgcac ggtcgttaat tgcgggtcgg
     6721 tgtaacgggc gatgggaata tcatcgaaac cgatgattga gagatgtaac ggaatcgcaa
     6781 tgccattatc ttttaatgct gtcagtgcgc cagcagccat attgtcgtta taagcaaata
     6841 cagcggtaag ttgtagattg cgccccagca gttcaaccat cgccgcctca ccgcccggca
     6901 tgtccggcgt accagtgcca atccagcttt ccggcggaat aatatcctgc tctttcaacg
     6961 cactcatcca gcctgcttta cgcatggcgt catcttcaat gccgtgactg gaagaaagat
     7021 aaccaatacg ttgatgaccg ttattcagca acatgcgcgt cgccattcgg gcaccgctga
     7081 gattatccag gcaaacgcaa cgatgggcgt accccggcac aacgcggttg attaacacca
     7141 taccgggaat gttatccata aattgcgcca gttcatcgtc actcaatgct tttgagtgaa
     7201 caatcaacgc attacaacgc tggcgaatta acacctcaat ggcgtgacgc tctttttccg
     7261 cttcatgata gctattgccg attagcacgt atttctgatg ctgctgagcg accagatcca
     7321 ccgcttttac cagcgcgccg aaaaacgcat cagaaacgtc catcaccacc acgccaatgg
     7381 tgtcgctaac ctgagttgcc agcgcctggg cattggcgtt tggccgataa tccagctcac
     7441 tcacggcttt cattactgct tcacgcgtgt cggcactgac cagcgtgctg ttattgagca
     7501 cccgggaaac cgttgccaca gagacgccag cctgacgcgc tacatcacga atggtgatca
     7561 tattcactac ctgttatgag attgcagtaa atgactgctt gctggcggct attttgtcag
     7621 cactgaatac aggacttcgt gaatcgagtc acagcaatgg aaacggttac agccgttttg
     7681 ttaacgaatg tgatccagat cgttatcttg atgttttaga tattgccgtt ccggcagcac
     7741 gcaaagttaa ctgacgccag agccattcca ccggcccctg gcggaagtaa cgcagccaga
     7801 taacagagaa gaggatattc gccagccata ccggaataac aaacgccagc agctccaggc
     7861 ggtcaaaatg cataaacaaa ccgaggtggt aaaaaagcat ggtacaaatc agcgtttgca
     7921 atagatagtt ggtcagcgcc atccgtccga cgcaggcgat cgcaagcacc agcttaaagc
     7981 ggctcaattg cggccagaag ccataaaaca gcgacgcata gccaatcgcc tgaaacggcg
     8041 cactcagttc ccgcggcatt tgaagtaaga aggcacacca gcgatacgcc cagtccagct
     8101 gccattgcag ggcgatggca ggaaggttaa tggtcacccc aatcgccacc agcacaaaac
     8161 cagtacgacg atagtgacgt aagctgaact gccctttcag ccagccgctg cgcatcagcg
     8221 ctgcaccaat gagcatcatc ccagccagtt gccagccata ctgtgcgccc agtgccagta
     8281 aactgttgcc aacaccatcg gcacgattac tgatcgcttc aacgccgccg tgaagcttcc
     8341 agtatttttc atacaaaata gccgatgcat ccggcgtcca ggcgcggctg gtctggctgt
     8401 cggaaatcag ccccaataac agcaaaacgc caagccccac cagataaagc atgacgcctg
     8461 tattaaacag gctttttacc gatggcgcat cgcgcaccag ccgccagcag attaagccca
     8521 ccagcccgta agccagcaga atatcgccgt cccagaacaa taaaccgtga ataaagccca
     8581 ataagactaa cagcgttaac cgcgactgga tccagcgtct gccacggggc agcaacattt
     8641 gcagtccagc accaaacagc agcgcaaaaa gcgtgaggaa tttcacctgg gcgatgagat
     8701 caagaaatgc ccaggtccat gcatcctgcg gcgtaatagc accgtaccag gcgggattga
     8761 gataagccgc ctttggtagc ccaaaggcgc tgatgtttaa tagcaggatc cccagaatgg
     8821 cgacgccgcg aacaaaatcg agcgtgacgt tgcgctccat ggttcctgcc ttttaatcag
     8881 ttgtgatgac gcacagcgcg cagaaactcg tggcgcgtat tctgactgga tttgaacaat
     8941 ccaccaagag aggtcgttgt tgtggcactg gttgcatcgc ggatgccacg cgccttcacg
     9001 cagtaatgca ccgcgtcgat cgagacagcc acgttattgg tgcccagcag cgtttgtagc
     9061 gcaataagaa tttgctgcgt cagacgttcc tgcacctgcg gacgctgggc aaagaactgc
     9121 acgatacggt taatttttga cagaccgatc accgaatctt tcgggatata agccaccgtc
     9181 gctttgccat cgatggtaac aaaatggtgt tcacaggtac tggtcagagt gatatcgcgc
     9241 acggtgacca tttcatcgac cttcattttg ttttcaatga gggtgatttt cgggaagttg
     9301 gcgtaatcca gaccggagaa aatttcatcg acatacattt tagcgatgcg atgcggcgtt
     9361 tccatcaaac tgtcatcagc caggtcgaga ttcagcagct gcatgatttc ggtcatatga
     9421 ccagcaataa ggcttttgcg cgtttcgtta tccatttcat gcacgggcgg gcgcagcggt
     9481 gtttccagtc ctcgcgcaac taacgcttca tgaaccaggg ccgcttcttt actgagtgat
     9541 ggcatttatg atttctcctg caggtgtgac gcctccgccc tgcgtggggg caaagttatt
     9601 aagctgattt acagcttgat tattgtgcgt gaggcggcgc acataatcca gtattcacag
     9661 cgataattat tgtaattgcc gctgcctttc atcagcagat gttaaaacat cgttatgcaa
     9721 atacggaagt gaaagttact cacagcacat tgaataaacg gtatgatgaa gaaattgcaa
     9781 acaacacaac aaggagccac gcatggaaat gctcgaagag caccgctgtt ttgaaggctg
     9841 gcagcaacgc tggcgacacg actccagtac cttaaactgt ccgatgacgt tcagtatctt
     9901 tctcccacca cctcgtgatc acaccccgcc accagtgctg tactggcttt ccggattaac
     9961 ctgcaatgac gagaacttca ccatcaaggc gggtgcccag cgcgtggcgg cagaactggg
    10021 gattgtactg gtgatgccag acaccagccc gcgcggcgaa caggttgcca acgatgatgg
    10081 ctacgattta ggccagggcg cggggtttta tcttaacgcc acgcaaccgc cgtgggcgac
    10141 gcattaccgg atgtatgatt atctgcgcga tgaattaccg gcgctggttc agtcgcaatt
    10201 taatgtcagc gaccgctgcg ccattagcgg tcattcaatg ggtggtcacg gtgcgctgat
    10261 tatggcgctg aaaaatccgg gtaaatacac cagcgtttcg gcctttgcgc caattgtgaa
    10321 tccgtgcagc gtcccgtggg gaatcaaagc gtttagcagc tatttaggtg aggacaaaaa
    10381 tgcatggctg gaatgggaca gttgcgcgct gatgtatgcc agtaacgcgc aggatgcgat
    10441 cccgacgctt atcgatcagg gcgataatga tcagtttctt gccgaccagt tgcaacctgc
    10501 ggtactggca gaagccgcgc gccagaaagc gtggccgatg acgctgcgta ttcagccggg
    10561 atatgatcac agttactact tcatcgcctc ttttatagag gatcacctgc gcttccatgc
    10621 gcagtattta ctgaagtgaa agtccgcccg gttcgccggg catcttctca tcagaagcga
    10681 taatccactg ccataaagta acgacgtccg tcttcgttat agctgtagtc gtcacgactg
    10741 agatccttgt cgccaaggtt cagcacgcct gcgcgcagtt tgacgtcttt agtcacctgc
    10801 caggccgcgc cggtattcca gatggtataa ccgcccggtg ttttagccgt cgcgctgtcg
    10861 gcgcgtttct gcccggtata gtgaccagaa acatagaatg accagtcttc cagcgccagc
    10921 ggtttccagt ccagcgtacc gttagcagta tggaacggca gatcggatag cggtttgttt
    10981 tcgccgttgc tgacatcacg accatcgttg taggtgtagt tgatcgacag tttccattca
    11041 tcgttgaacg gaattttcag ttcggtttcc acgccctgaa tacgagcttt gttaacgttg
    11101 tagtagctaa ataccggtat gcgccgtccg ttagcgcccg tctcaaaacc aacaaagttt
    11161 tggtagcccg gtgcagcgtt gacgtcagac gtacggctga tgctgatacg atctttcaca
    11221 tcgttacgga aaacggtaac gctggattca accccttcca gccagccttc ttcacccatg
    11281 tagtaaagcc ccagctccca actttcgctg gtttctggtt tcagatccgg gctacccaca
    11341 atcttacatg cgccacggca ggaattgctc gtccagtcag ggctaagttg caacagagaa
    11401 ggtgctttaa atgccgtcgc ccagccccct ttcaccgtta cggtgtcggt ggcgttataa
    11461 accaggtagg cacgcggact ccagtgttca ccgtaggttt cgtgatcgtc catacgcacg
    11521 ccggtcgtca gcgccagcgg ctcgaagatc cgccattcat cttccacaaa cagcgcgtac
    11581 tggctggcag acgttttgga gctggttccc ccggtcaggt tcaccgcatc gctaagtttg
    11641 tcgtgacgcc attcaccgcc aaccgtgaga aactgattaa ttgccgtcag cggcaacgtg
    11701 tatttgccgt cgaccgtatt gctttcggaa gttatcgggc tgctgttgcc agggtttttg
    11761 ttctcgactt tctcaccgta gtatttcagt tcgctggtgc cgtaatccca acgcccatta
    11821 tggctgacgg agtagttctg gcgttccagg cggtttttgt ccagcgagtc ggaatcacga
    11881 tcctgacggt cgaaaccgta tccggcagta aaatcgtgat tttgattcgg tgtccaggca
    11941 aattcgacat tgccgtcgcg gctggagaat ccttcaatac gcggcgtttc tccggtatcg
    12001 gtggtcgttg agttttgcgg gtcatccttt tcacgttttg ccaggctgcc gtaagctttc
    12061 attcccagca caccatcaat taatggtcca ctggtaaaga actgaccgtt ataggtatca
    12121 ccgcgatcgc gatgttcctg aatggtggta tcgacggtaa cggtacccga ccatttctga
    12181 ccgatttttt tggtgatgat attcactaca ccgccgagcg catcggagcc gtatagcgac
    12241 gacatcgggc cacgaaccac ttcaatacgt tcgatggaat cgaccgggat ccagttcaga
    12301 tcgaaatcat tgtggcggaa gacggcattg cgggagttaa cgcgtttacc gtcgacgaga
    12361 atcagggtgt agctgctgtc cagaccacga atactaacgc ccttacggtt atccccttcg
    12421 ttcgtcagtt gtacgccagg cacttctttg aggacatcct tcagattctg taccggtttt
    12481 cgctgcaggt cttcctgggt aatgacgctg atactggcgg gtgcatcttt gaggttttgt
    12541 tcaacggaag atgcagtgac aaccatcgtt tcgccatcat catcgaccgc taacacaggc
    12601 catgcacaag aaatagcgga caaacacagc ccgacccgta cgaaagggtt caacctaaac
    12661 attccatatc tccatgaggt aactacgaaa ataaaatggg ttatcgctca catcttcttc
    12721 acgtcccctt gcgtacggca gcatcgcggt ggacttattt gtatgcaggt gatcatccca
    12781 gaaagccgga acagcctctg atttgtcagc cttttttgat tgcggcgtaa cgataatgca
    12841 aacgataaca attatcaatc caaatgttaa attttatatc cgcatgattt gtcagggaaa
    12901 tggaattgaa aaagccctct cggttgagag ggcttagcaa ggaagggagg aaacttattt
    12961 cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgagttc ctttaatcag
    13021 cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc
    13081 gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag tgataatcag
    13141 acacagaatg aatgcgaaga tcggccccag tgggaagaaa cctgaacggt acggcagatc
    13201 gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc taatggcaat
    13261 ccccagccag gcgataaaac ccgtcatccc ggaggtgttc agcagccaca ggtataccgt
    13321 ctggttgcca aacatggagg tcaggaagca cagaccggca atcaccgtcg tcgcatacag
    13381 cgcattacgc ggcacgccac cacgcgacag tttagcgaaa atgcgcggcg ctttaccgtc
    13441 acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac ccgctgacag
    13501 caccgccgtc agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg
    13561 gaacaccagg gtgaacggac taacgctgat gtctttaaca tcgttacgca gcaggctcgg
    13621 atcggtgtac ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat
    13681 tcgccagaac acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc
    13741 tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc
    13801 gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc
    13861 cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac
    13921 aataactgtc gtgactttga tcagtgagaa ccagtattcc gcttcaccaa agccacgaac
    13981 tgagatgtag ttcagcagga agataacgcc gaggaacaac gcactccaga tccagcccgg
    14041 tgtatccggg aaccaccagc tcatgaccag ctgagctgca accaggtcaa cggcgatagt
    14101 caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata
    14161 gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca gttcaccgag
    14221 actggtcatc aggaagtaaa ccatcaggcc aatcagcata tacgagagca atgccccgcc
    14281 cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc
    14341 gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc ccggcgcttc
    14401 tgtggtttta gtttcggaaa ccatacaaaa atgctatcca tcttaaaaat gaggcgcgat
    14461 tgtagcagac gattggcaat ccttccggca gaaatacacg gttataagac accttcatga
    14521 tcgcccaggg attataagta aagcagccaa tctcttttct ggcgaatgca tgctgagggc
    14581 gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt acagcaactt aacgcggtac
    14641 attcgcggga tcgcaatagt ccagaaagcg ccgtagcgcg ttggaaaggt gtttttgccg
    14701 atgatgtata cgccacaacg tacgcatcag gcgcgggaga gggaccgcaa cttcacttaa
    14761 tgtgcctgcc tgcaattgat ctttaatcac ccgtcgcgac aggcaactaa ttcccaaccc
    14821 atgacgcacc gcatgtttga ttgcctcgga gttacctaat tccatcgcca tctcaaactt
    14881 cggtaaatgt gacagcaaca gataatcgac aatctcccgc gtgccggaac cgcgttcacg
    14941 caggatccac ggcgcagcgg ccagctgttc taaggtgacc ggaccacgag ccaacggcga
    15001 agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa tgatttcagt
    15061 gctgtggcac ggtccttcaa taaagccaat atcaacgcgg aaatccagca ccgcttgcat
    15121 cacgtcctgg ctgttcccaa cgctaagttc aatcggcaac tgcggataat catggcgata
    15181 acgggcgata actgcaggca gaatgtagtt accgatggta ctactggcat agatacgaat
    15241 cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa gcaatgccaa
    15301 cgcacgcgga tagagcagcc gcccgtgttc attaacaacc agtcttttcc ccacgcgatc
    15361 aaacagttgc acgccaagct gcccttccag gtcggtcaag gctgcgctca ctgctgattg
    15421 cgacaacgcc agcatcaccg acgcctgggt ggttgatcca cttttcaata cttctgcaaa
    15481 aacttccaac tgccggaggg tgatgtgcat agtcgcttac cacttataaa gattaattat
    15541 aaatatataa tcaattttat ttttaaacca gttagtcgta accttatgcc cggttaaagg
    15601 agagggttat gacgaatatc accttacaga aacaacatcg tacactgtgg cattttattc
    15661 cggggttagc cctgagtgca gttatcaccg gggtcgccct gtggggtggt tccattcccg
    15721 cggttgcggg tgccgggttt agtgccctca ccctcgcaat cttgttgggg atggttttag
    15781 gcaacaccat ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg tttgccaagc
    15841 aatatttatt acgtctgggt atcattcttt atggcttccg tctgacgttc tcgcaaattg
    15901 ccgatgtcgg tatcagtggg atcatcattg acgtgttgac gctgtccagt accttcctgc
    15961 tggcttgctt cctggggcag aaagtgtttg gtctggataa gcacaccagc tggttgatcg
    16021 gtgccggtag cagtatctgt ggtgctgccg cggtactggc gactgagccg gtagtgaaag
    16081 cggaagccag taaagtaacc gtggctgttg caaccgttgt tatcttcggg accgtcgcta
    16141 ttttcctcta cccggcgata tatccgctga tgtctcaatg gtttagtccg gaaactttcg
    16201 gtatctacat tggttctact gtgcacgaag tggcgcaggt ggtggcggca ggtcatgcca
    16261 tcagcccgga tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg atgatgctgg
    16321 ctccgttcct catcctgctg gcggcgcgtg ttaaacagct gtctggggcg aacagcggcg
    16381 agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt catcgtagtt gccatcttta
    16441 actcgttcca cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg gataccttcc
    16501 tgctggcaat ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg ctgaaaaaag
    16561 ccggggcgaa acctctgctg atggcactgg tactgtttgc ctggctgatt gttggtggtg
    16621 gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac cactacatct tgctcctgtt
    16681 aacccgctat cattaccgtt ttcctccagc gggtttaaca ggagtcctcg catgaaatac
    16741 attggagcgc acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg cgccgccgaa
    16801 atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg tgccgcaccg
    16861 ctcacgacgc aaaccatcga tgaattcaaa gcggcctgtg aaaaatatca ctacacatcg
    16921 gcgcaaattc ttccccacga cagctatctg attaacctcg gacatccggt cactgaagct
    16981 ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca actggggctt
    17041 tctttgctca acttccaccc tggcagccat ctgatgcaga tttcagaaga ggattgcctt
    17101 gcgcgtattg ccgaatccat caacattgcg ctggataaaa ctcaaggtgt aacagcggtg
    17161 attgaaaaca ccgccggtca gggcagtaac ttagggttta aattcgaaca tctcgcggcg
    17221 attatcgacg gcgtggaaga taaatcccgt gtcggcgtct gcattgatac ctgccatgct
    17281 ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg aaaaaacgtt cgctgatttt
    17341 gcccgtattg ttggctttaa gtatctgcgc gggatgcacc ttaacgatgc gaaaagcacc
    17401 tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag gcaatatcgg tcatgatgcg
    17461 ttccgctgga tcatgcagga cgaccgtttc gacggcattc cgctgatcct tgaaaccatc
    17521 aacccggata tctgggcaga agagatcgcc tggctgaaag cgcaacaaac tgaaaaagcg
    17581 gtggcctgat cgatgaacga cagggaaaag cagattctta aaattttacg gcgtaacccg
    17641 ctgattcaac agaatgaaat cgccgatatt ctgcaaatca gccgctcacg tgttgccgct
    17701 catattatgg atttgatgcg caagggtttg attaaaggca aaggatatat ccttaccgag
    17761 caggattact gtgtggttgt gggtgcaatc aatatggata ttcgcggaat ggcggatatc
    17821 cgttacccgc aagcagcctc tcatccaggt tctgttcact gttccgcagg cggcgtcgga
    17881 cgtaatatcg cgcataacct ggctctgctt ggtcgggatg tgcatttaat atcagctatt
    17941 ggcaacgatt tttacggcga gacattactg gaagaaacgc gccgcgcggg cgtgaacgtt
    18001 tcaaactgca ttcgtctgca tggacacagc actgcaactt atctcgctat cgctaataag
    18061 caggaagaaa ctatcctggc aatcaatgac acacatattc tgcaacagct aacgcctcaa
    18121 ctgctcaata cttcacggga cttgattcgc catgctggtg tggtgctggc tgattgtaat
    18181 ctcacacctg aagccctgga gtgggtcttt accattgctg atgaaattcc gatgtttgtc
    18241 gataccgttt ccgaatttaa agcgaacaaa gtaaaaaact ggtacagccg gatccacact
    18301 ctgaaaccaa cacaaaatga actggaaatt ttgtgggggc aaccgattaa agacgacaac
    18361 gatcgcatca gagctgtaaa ttcgctgcat caacaaggtg ttaaacgcat ttttgtttat
    18421 ctgaaagatg aatctgtatt ttgcagcgat aaggatggag aacaattttt gctgaccgcg
    18481 ccagcgcata cgacagtaga cagttttggt gctgacgatg gttttatggc gggcctggta
    18541 tatagctttc ttgaaggata cagtttccgc gacagcgccc gttttgcggt agcctgcgcg
    18601 gcaatttcgc gcgccagcgg cagcttaaac aaccctaccc tgtctgccga taacgcgctt
    18661 tcattagtgc caatggtgta acaatgttgc cggattcgac gcttgaccgc atcttatccg
    18721 gctcataaat tgcgcactac gctaaaccaa taaagaatcc tgcaatagta gcactcatca
    18781 ggttagaaag tgtcgccgcc gccagcgcgc gtaaaccaag ttgggcgatt tccggcgcac
    18841 ggtgtggcgc aaccgcagaa aacgccccca ccaccacccc gatagaacca aagttagcga
    18901 aaccgcacaa cgcgaaagaa ataatcgcca cggttttagc atccagagtg ccacccgttt
    18961 gcagatatgg cgagaaattg agataagcga caaattcatt gatcgccagc ttctgcccaa
    19021 tcaaactccc ggcaagattt gcatcactcc agtcaacccc catcacccac gccaatgggg
    19081 ccaacaggta acctaaaatg gactccagcg aggcatgtgc aaaaccaaac cagccgccaa
    19141 cgccgccgat aataccatta attaacgcaa tgatggcgac aaatgccata acaactgtcg
    19201 ctacaccggc ggcgattttc agcccggtca ttgcccctgt cgcagcggct tcaataatgc
    19261 tttttggcgg tgtttcggtg aaagagaggt tattaaagga aacctgcgaa gattccgtag
    19321 ccgggcttaa caggcgggca aacaagatcc cgcctgggat cgccattaac gatgccgcca
    19381 gcaaatattc cacaggtacg cccagggcgg cgtaaccaat cattgtcgaa ccagcaatcg
    19441 aggccatgcc actacaaatc gctgtaaata attcattgcg attcagacga tcgataaagg
    19501 gtttgacgat tgccggaatt tcgttttgcc cgaggaaaat ggtagtgacc gcgacgaatg
    19561 actcgatctt gctgatattt aacgctttct ggaagatacc gccgagaatg cgaattaaaa
    19621 tccccatcac accgatgtag tagagaatac tcaccagtgc tgtaacgaag ataattgccg
    19681 gtaatactct gaaaccaaag ataaatcctg cgccatcaaa cagcgtgtcc attttcggcc
    19741 cgaccagcga accgaagata aatgcgctac ccgcgtcgct gtacgccatc actttatgca
    19801 cgccaaaagc gactttttca gcgacccaac gccctggcgg taaccaaagc ataatgccgc
    19861 caatcacaac ctgtaacact aacgccgcgc caacggtacg caggctgatc ttcttcttgt
    19921 ttactgacag caaaaatgca attgccagca ataccaccat tcccagaaca cttctcatga
    19981 catccatagt gattatctct tcatgtcata ttgggcgacg gctttctgcc catcagaaat
    20041 agccgggcct gcatcttgcg ctatcggccc ggcttgttat caacgcttaa tgggttttga
    20101 tgtagccgcg cacgcactct tcgactaagc cccagaacca gtcggtatca atagtgatgc
    20161 cgactttggt attggcgggc ttgccaagaa cgcccagctc gtcgcagacg gtgcgcccat
    20221 agcaagggcc actgttgacg tccacttcga cgtacatctc ctgggtttta atgccatcag
    20281 ggttaatcag ataaccgatg caggtggcgt cgtgcaccgg gccgccagcc aggccgtagt
    20341 tttcgaactg cgttttgaga gtgaagttca tgatgtcgct gaacagctct ccggcagggc
    20401 cgcctgccct ttccatccga gcaatcacgt ccggggtgca aacggtctgg ttggtgagat
    20461 cgaggcccat catcactaat ggaacgccag aggtgaacac cacgcgggct gcttccgggt
    20521 cagcaaagat gttgaactcg gcagatggcg tgaagttgcc tgtaccgtaa gcgccgccca
    20581 ttagcacaat ttcacggatt ttgggcagga tcgcgggttg catacgcatt gccaccgcga
    20641 tatttgaaag cggaccaacc ggcaccagag tgatatcgcc atcgcttgcc atcagggtat
    20701 caatgatgta tttcaccgcg tgggtacttt ctgcctggcg ggttagcggc tcgaataccg
    20761 ggccatccag tccggtttca ccgtgaatat tatcggcaac gatttgttga cgcataatgg
    20821 gttgcggcat ccccgcgtaa accggaacat taatctccag tttctggcaa acattcaggc
    20881 cattaattaa tgttttatca agcgtctgat tacccgctac aatagtgatg cctaataaat
    20941 ctattgccgg atgtttcgct gccatcatca ttgcaatagc atcatcatga cccggatcac
    21001 aatccagaat aatttttctc ttttccattg tttatttcct ctgtttccag ttgcgttatt
    21061 ttttctacag caaagaaaat tcgaccgggc cgatgattga atcttaccaa ccgcgtacgt
    21121 atgctaaata tgagaaatct catagcggat aaacatcgtg aaagaaatcc acaataatga
    21181 tcttaagcag caattgatga gtgaatccgc gtttaaggat tgctttttaa cggatgtttc
    21241 agccgatacg cggctgtttc attttttagc gcgtgactac attgtgcagg aagggcaaca
    21301 gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg ctttacgcca cgctagctaa
    21361 tggtcgcgtg tcgctgatcg atttctttgc cgccccctgt tttattggcg agattgagtt
    21421 aatcgacaaa gaccatgaac cgcgtgcggt gcaggctatt gaagagtgtt ggtgccttgc
    21481 gctccctatg aaacattacc gcccgcggtt attaaacgac acgctatttt tacgaaaact
    21541 ctgcgtcacc ttaagtcata aaaattatcg taatattgtt tctttaactc agaatcaatc
    21601 atttccgtta gtgaatcgcc tggcagcatt tatattactc tcgcaggaag gtgatcttta
    21661 tcacgaaaag catacgcaag cggcagagta tttaggcgtt tcttatcgac atcttttata
    21721 tgttctcgcg caattcattc acgacggttt attaattaaa agcaagaaag ggtatctaat
    21781 taaaaacaga aagcagttgt caggactggc gctggagatg gacccggaga ataaattctc
    21841 cgggatgatg cagtaaaaat tatttgcaat agcgcgattg ccggatgcaa cacttaataa
    21901 gcgcgtacat ccggcaatga aactcaagct aaaccaataa agaacccggc aatggtcgca
    21961 ctcatcaggt tggaaagcgt tgctgctgcc agcgcccgta aaccaagctg ggcgatttcc
    22021 ggcgcgcgtt ttggcgaaat agccgaaaat gcgccaacga caacaccgat agaaccaaag
    22081 ttagcaaaac cacaaagcgc aaaggagata atcgcaatgg ttttcacttc cagcgtgccg
    22141 cccgtttgca ggtatgggga gaaactcagg taagcgacga attcgttaat cgccagtttc
    22201 tgcccaatca ggctacccgc aagattggca tcactccagt ccacacccat gatccacgcc
    22261 agcggtgcca gcacatagcc aaaaatactt tccagagagg cattggcgaa accaaaccag
    22321 ccaccaattc cgccgataat gccgttgatc agcgcaataa ttgcgacaaa cgccattacc
    22381 accgtcgcta caccagcggc gattttcagc ccggtcatcg caccgctcgc cgccgcttcg
    22441 ataaagcttt ttggcggcgt ttcgctgaac gacagatttt caaatgtgac ctgtgaaggc
    22501 tcggtggccg ggctaagaat acgtgcaaac aaaataccac ctgggatcgc catcagcgat
    22561 gccgctaaca ggtagtcaat tggtacgccc attccggcat aaccaatcat catcgaacca
    22621 gcaatggacg ccatcccgct acaaattgcg gtaaacaact cgttgcgatt catgcgatcg
    22681 ataaacggtt taacgatcgc cgggatctca ttttgcccga ggaaaatagt tgtgactgcg
    22741 acaaaagatt cgattttgct gatgttaaga gctttctgga aaatgctgcc aaggatgcga
    22801 atcagcagcc ccatcacgcc aatgtagtac agcagactga tgagcgcagt aacgaaaata
    22861 atcgccggaa gtacgcgaaa ggcgaagata aaacccgcac cgtcaaacag gacatccatt
    22921 ttcggcccaa ccagcgaacc aaaaatgaag gcgctaccgg catcactgta agacatcact
    22981 ttatgaacgc ctaatgccgc ctgttctact gcccactttc ccggtgggaa gtagagcatg
    23041 atgccaccaa tagcgatttg cagcagcagt gcggctccaa ccgtgcgcaa actgatgctc
    23101 tttttattca ctgacaacag aaatgctatt gccagtaaca ccaccatccc cacaacactt
    23161 ctcattatat ccataatgat tttcccttca tgccagtaaa cccggcgtca gcgccaggtt
    23221 ttggtatgct tgatgagtac gggcgacggc tttctgcccg tcagaaatta acccgcgaga
    23281 cgctggtatt ctttggcaat ttcactcgcc agaatggcgt tgttgaacac cagctggata
    23341 ttggatttca ggctgtcacc gccggtcagc tcagcaacgc gcgccagcag gaacggtgta
    23401 ctttctttac caataacgcc ctgctcttcg gcttcggcga cggcttgatc tatggctgca
    23461 ttgatagatt cctccggcat cgcaaactgt tccgggatcg gattcgccac taccaggccg
    23521 ccgttcaaac cggtttgcca tttcactgcc attgcacgag cgatttccgt ggcactatca
    23581 aggcgaatac tgacttcaaa tgggctggta cggcagaaaa acgcaggcag cgcttttgtc
    23641 tgatagccaa ttaacggcac accgaaggtt tctaaatatt ctgttgttaa tcctaaatcg
    23701 agaatagatt tcgccccggc acaaacaacg gtgacattag tatttgccag ttcttgcaaa
    23761 tcggcagaaa tatcgaaggt atgttccgcc ccgcgatgca caccaccaat tcccccggtg
    23821 gcaaatactt taattccggc aagcgccgca ataatcatcg ttgaagccac agtggttgcg
    23881 ccattttttc cggcggcaac aacaaaaggt aaatcgcgac gactaacttt ggtcacgtta
    23941 tgcccttcac gacccagtaa ttcaatttct tctttgctta aacccacttt catcacgccg
    24001 ccaataatgg cgatagtggc aggtactgcg ccctgtttac gaatagtttc ttctacttca
    24061 attgcggtct gggcattttg tgggaacggc atcccgtgag aaataatggt cgattccagc
    24121 gccacaaccg gttttttgtt ttttaaagcg tcctgcactt ccggggaaat ttgtaataat
    24181 tcaggggaaa tttttaattc agacattctg cgttctccac taacgatata acgttggcaa
    24241 tcgataaatc ggggttattg gtgtattcac aggagagcgc cattgacgaa catccctgtg
    24301 cgaaacgaac agattcggca aacggcattc cgtctaccca acacgaagca agtcccgcca
    24361 tcatggcatc gcccgctccg gtaacattaa taacattggt tttgatcggg gcagaccagc
    24421 cactttcacc gctgatatca ctgtaataaa cgccatcgcc gcccatgctc aataccagtc
    24481 ggttcaggcc atgttgatgg aaccaggcag caacttttgc cacatctcca cgccctgaca
    24541 gcgcaatccc actcagtgtt tccgcttcaa ggcggtttgg cttcagagtg tggatctgat
    24601 ttagacggtc gcgcactttg acacatttcc acgctgaaac cggatcgaca aatacgggta
    24661 cgttggcagc attatccaga atccatgcca gtgcctcttc actgatatta cagtccgcga
    24721 caatgacctt tgccctctga ataaattcac ggtgctgtgc gagatattca gctgtaatag
    24781 cgttgctaat attcatgtca tttatagcaa ccagcatttc accggtatta tcgagtaatg
    24841 ataaataact cgacgtattt tctcccggca caatcaggca tttatcgaca taaacgccag
    24901 attgattggt ttgcgttagc agcgattgac cataaaaatc actacctact gcgctcagta
    24961 accaggcttt gttacccagc aacgccaggt tttgtgcaat atttcgccct actccaccag
    25021 gtgtaaattt tattttacct ggatttgaat ccgcataatt taatgattca tgtgaatatc
    25081 cggcgacatc aatattcgcc gaacctataa ttacgacata atccttttcg cgcatagcac
    25141 gtcctctggt aaaaaattac caaccctgtc aatccacaag aatatagata ctgcaaagac
    25201 agtttaaaca tgtgttcata tttaaacatg tgctcatagt atgtatgttt tacgtaaagt
    25261 aaattgccgc cagagaggca aagcgggcag atttgagcag gctcacaaaa tgcgattaaa
    25321 tgccagaatt acaaaagcga ggaatttgtg acgggatgca cagaacagga acactaaaaa
    25381 agggcagaaa agaatctgcc cttgaggaaa taaccttagt gtttgatgaa cgtttcaggt
    25441 ttctactgaa agataaaacc atttaaattc ataaaattac attcacaaac cggtcattct
    25501 ctttgcgctc atccaatgat acagggcagt aaatttctgc cctgtaacac accttttatt
    25561 acgcggcttt cgctactgcg tccacttccg gacgtttcag gaaggcatag gccaaacccg
    25621 ccaccagcgt accggcaata attgctacca ggtaacccag taccggcgta atagcgccag
    25681 ggatcagcag aacaaacaga ccaccgtgcg gtgccatcag tttcgcacca atcgccattg
    25741 agattgcgcc agtcagcgcc ccaccaacga tacaacacgg cagcacacgc atcggatcac
    25801 gagcagcaaa cggaattgca ccttccgaaa tgaagcacag tcccaatacc agagcggctt
    25861 tgccaccttc ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca
    25921 ttgccagcgg tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag
    25981 tactcagcag acccacaccg aatgcgtacg ctgctttgtt taccggaccg cccatgtcag
    26041 tacacatcat gccaccgagg atcgccccca gcagaaccgc attcgcagtc cccatggtct
    26101 gcagccagtg agtcaaccct tcgagaatgc cagcaactgg tttaccgatc aggtagatca
    26161 tcgccagacc gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagcgcct
    26221 ccatactctg tggcagtttc agttgcgtac tgattaactt cgcaatgtaa ccagccagga
    26281 agcccgcaat aataccgcca atgaagccag aaccggtgct gaccgccagc ataccgccaa
    26341 tcagacccgg cgtgagaccc ggacgatcgg caatggagaa ggcaatgtaa cctgccagta
    26401 ccggcaccat cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg
    26461 tacccggctc tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac
    26521 ctgcaacgac catcggcagc atgtaagaga cgcccgtcag caagtgacgg tatgcgcctg
    26581 cactctcttt cttaccttca gaggtcgccg tttgagcttt gcccgccggt tcatacggcg
    26641 ttgcttcagc aaccgcttta tccagttcct gcgcggtttt cttcagcgcc agaccggtag
    26701 aggtacgata catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca
    26761 caatcaccag atccgctgcg gctacttctt cgggggtgat tgcattaccc gcaccaacag
    26821 aaccacgggt ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt
    26881 cagccgccat aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca actacgcgtt
    26941 tcggaccgct ggcggcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa ggtttcgcat
    27001 ggcctttggc ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa atatcaccca
    27061 gccagacatt tttaccgttc agcgcgctgt cattcgggat ggaatcaccg agaacaatcg
    27121 ccatttctgc gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc
    27181 ccagcagggt cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca
    27241 gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc
    27301 attgcggcca actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct
    27361 gcaacagcag tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa
    27421 atcaggccac caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt
    27481 ggtttggcga tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa
    27541 ataacaacat gcgcaatacc ttgttcacgt agcgcatgtg cagcttcaat cacatctttc
    27601 atttcaggca gtttacggcc tgcccagatt tccaactcgc ggcggttagg tttcaccagc
    27661 cacggtgccg ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa
    27721 ggacactgac tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac
    27781 ggtaagcttc cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc
    27841 acaaagcgtt cccagtcggc gggggtgact tcaaaacccg agaagttgaa gtcggtcact
    27901 tcgccgtctt tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa
    27961 cggttggcaa tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccc
    28021 aggaagccgc caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg
    28081 cctttacccg ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc gcgttcaatt
    28141 tccgggcaga aaccaacaag gtcataagcc ggattaaggg tgatagtagc aacacgtctg
    28201 ctcattatgc gccctcccca agaccagcag cgatagcgtc gccgattgct ttcagcgcct
    28261 gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg
    28321 ccacaacttt catcagacta cgtccgtttg ccggtttgcc ggtgccatca aggtttgtca
    28381 cggtaatatc actgttaaat tgtttaatgg tgttgaccag catggtacct ggacgagcat
    28441 gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg gtcggcgcat
    28501 catcgctggt cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt
    28561 tattgtcgag caacaaatca gcgagacgct taagaaccgc aatgggctga tcgtcattca
    28621 tcgccacact caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac
    28681 ggcttaccgc aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct
    28741 gtccgaggtt cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc
    28801 tcgcctcttt cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcggcga
    28861 tatccagcgt cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca
    28921 gcaatgcgcg aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat
    28981 cgctcagtac gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa
    29041 taccgatcgc tacgtacgct acctgaccgt caccccaggt gacgccttcc gtgaactgaa
    29101 atacctgaac gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag
    29161 caataccatt gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt
    29221 agccttctgc tacgttaccg gcctgcacca gcgccgcagc gagctggcga atcgcctctt
    29281 ctttgtctcc ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc
    29341 tcctctcttg ctgaattgaa acgattcagc ttctatgaga aaaaaagcgc caacctggct
    29401 tagggttaaa gacaagatcg cgctgaaacg tttcaagaaa gcataatact tctgtttcag
    29461 cacgcaagga aagccgtaaa gttatgagca aaagtttgat gtgctgcaca attttttgtc
    29521 atttttccta attgctgatg ggaaaatccg ttatcagcaa tttcatttca gcagcgtcag
    29581 cagccaatca gcaacagtga aatggcattt tgattattcg tggcgctttt gatttcgctg
    29641 tctgtttatt ttctgacaag cagcgtaaac tccgcgtctt cctcttccag tgatcgacca
    29701 gcatgcataa ctcccccgca gtctccagcg cgaaatcgtt tgacctgacc tcgacggcgt
    29761 ttttaatcgt tgcctttctc accggtattg cgggcgctct gcaaaccccg acactcagta
    29821 tttttcttac cgatgaagta catgcccgtc cggcgatggt gggattcttc tttaccggca
    29881 gcgctgtcat tgggattctg gtaagtcagt ttctcgccgg gcgctctgat aagcgcggcg
    29941 atcgcaaatc gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc accctttttg
    30001 cctggaatcg caactacttt gttttgctat tcgttggcgt ctttcttagc agctttggct
    30061 cgaccgctaa cccgcaaatg tttgcccttg cccgtgaaca tgccgacaaa accggacgtg
    30121 aggcggtgat gttcagctct tttttacgcg ctcaggtttc actggcatgg gtcattggcc
    30181 caccgctggc ttatgcgtta gcgatgggtt tcagctttac ggtaatgtat ctgagcgcag
    30241 cggtagcgtt tattgtttgc ggcgtgatgg tgtggctgtt tttaccgtcg atgcaaaaag
    30301 agcttccgct ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc gatacgctac
    30361 tgctgtttgt catttgtaca ttaatgtggg gctcgaacag cctgtacatc atcaacatgc
    30421 cgctatttat tatcaacgaa ctgcatcttc ccgagaaact ggccggtgtg atgatgggga
    30481 ccgccgccgg gctggaaatc ccgacgatgt tgattgccgg atatttcgcc aaacgtctgg
    30541 gtaagcgttt cttaatgcgc gttgctgccg tgggtggcgt ctgtttttac gcaggaatgc
    30601 tgatggcgca ttcacctgtc attctgttgg gcttgcagct gctaaatgct atttttattg
    30661 gcattctggg cggcatcggg atgctctatt ttcaggatct gatgcccggt caggcgggtt
    30721 cagccaccac gctctatacc aacacttcgc gcgtgggctg gatcatcgca ggatcagtgg
    30781 cgggcatcgt cgccgagatc tggaattatc acgctgtgtt ctggtttgcg atggtgatga
    30841 ttatcgccac tctgttttgc ttactgcgga ttaaagatgt ttaaggcgcg gtcagcattt
    30901 ccagatcgat aagccaggtc atggcctgct gacgcgagtt accgcacatt tcagcgctgg
    30961 cttgtaagcc tgcacagact ttcggtcgca gtggcgaggt gaaaatttta cagcgttgct
    31021 gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc atctggcata ccgggaatag
    31081 ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc cggacggcat tccatacgtt
    31141 gttctcttaa ggttaaccac cggattgatg agcgcgcaca gtaccacctt ttttgcacca
    31201 gcaaaagtgc gaataccact tgcccgaaag gcccgtcgcg agtactttgt cgcgatattt
    31261 ttgacatttt cgactacagg aatttttcga tgccaagagc gaacgaaatt aaaaaaggta
    31321 tggtactgaa ttacaacggc aaactgctgc tggttaagga tattgatatt cagtcgccca
    31381 ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt accgggctga
    31441 aagtagaaga gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg acccgccgtt
    31501 acgttgactt ctcctatgtc gatggcaacg aatatgtctt tatggataaa gaagactata
    31561 ccccgtatac cttcaccaaa gatcagattg aagaagagtt gctgtttatg ccagagggcg
    31621 gcatgccgga catgcaggtg ctgacctggg acggtcaact gctggcgctt gagctgccgc
    31681 agaccgttga tctggaaatc gttgaaaccg caccaggcat caaaggggca tctgccagcg
    31741 cccgtaacaa accggcgaca ttgagcactg gtctggtgat tcaggtacca gaatacttaa
    31801 gcccgggcga aaaaattcgt atccatatcg aagaacgccg ttatatgggg cgtgctgact
    31861 aacttcagcc gcatgcagaa aagggatagc tcaggctgtc ccttttttaa tttattatac
    31921 caatcttcta ttttgcgctt cctgaacacc ttacgccctc aattttcact cgttgattga
    31981 tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg tcatataact
    32041 ttacactgtc attgttaatt tatcgttact aagacgtgac tcctatgaag acaattgcct
    32101 ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag ttgcaatcac
    32161 gtatcgttca ttttggcttt ggcgcctttc accgcgctca tcaggcgtta ctgaccgatc
    32221 gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga aatcagcttg ttcagcggtg
    32281 atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg gagaaaggtg
    32341 cggacggcaa tcaggtgata attgtcggtg ccgttcacga atgccttaat gcaaaactgg
    32401 attccttagc ggcaattatt gagaaatttt gcgagccaca ggtggcaatt gtttccctga
    32461 cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac accagtaatc
    32521 cgcggattat tcacgatcta caaacccctg aagaacctca ctccgcaccg ggtattctcg
    32581 tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg ctctcctgcg
    32641 acaatattcc cgacaatggt catgtggtga aaaacgcggt gctgggaatg gcagaaaaac
    32701 gttcgccaga actcgccggg tggataaaag agcacgtcag ttttccggga accatggtcg
    32761 accgcattgt tccggctgca accgacgaat cactggtgga aatcagccag catctggggg
    32821 tgaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtg gaagataact
    32881 tcgtcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat gatgtcctgc
    32941 catgggaaga gatgaaactg cggatgctta atggcagcca ctcttttctc gcttatctgg
    33001 gttacctctc aggattcgcc catatcagtg attgcatgca ggatcgcgca tttcgccatg
    33061 ccgccagaac attaatgctg gatgagcaag cgccgacact gcaaattaaa gatgtcgatt
    33121 taacacaata tgcggataag ttaattgcac gttttgctaa tccggcgctg aaacataaga
    33181 cctggcaaat cgcgatggat ggcagccaga aattaccgca acgcatgctg gcaggtattc
    33241 gcatacatca ggggcgcgaa acggactggt cgttgctggc attaggcgtt gcaggctgga
    33301 tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc gatccgctta
    33361 gcgataaaat tcgcgaactt gttgcgggca gcagcagtga acaacgcgta accgccctgc
    33421 tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt gtgcaggcca
    33481 tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta ttaaacaccc
    33541 tcaaaattta acgatttctg cggttaaagc ggatgaagct caccttcgtc cgctctcccc
    33601 ttctcttttc tgcttttttt agccaggatt aacgctcagt taacttacca gagtattgcg
    33661 gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg aaaaccacgt
    33721 cgattcttca tctgttagcc cataaagatc ccaacgaaaa atgggcggta ctggttaatg
    33781 aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca ttgctgaaag
    33841 agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta gggttgaata
    33901 ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg ctgggccatc
    33961 cgaaacagat cctcgatctg ttaaccgcac cagtctatga accgtggata gatctgcgcg
    34021 ccaccttgtg cattctcgat ccgcgcttgc tgctggacga aaaaagcgcc agcaatgaaa
    34081 acttccgtga ccagctggct gccgcagaca tcattgtcgc caataaatcc gaccgtgcga
    34141 cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc gatcgacaat
    34201 taattcacag tgagcatggg aaagttgacg gtcatcttct ggatttgccg cgtcgcaatt
    34261 tagccgagtt gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa aaagggttag
    34321 cagcgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc gggcaaggat
    34381 atcaggcctg cggctggata ttcgacgctg atacggtatt cgacaccatt ggcattctgg
    34441 aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt cccgaagggc
    34501 tggtacgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac gttgcgccac
    34561 cggacagccg tattgagctg atttccagca gcgaagctga ctggaatgct ttgcagagcg
    34621 cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt tcagtaagat
    34681 aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata ttgtcggcct
    34741 cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc cgattgatgc
    34801 ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt tgtggctggt
    34861 tgcattgacc aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg gaatgttgat
    34921 gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga ttatgggtct
    34981 ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa ttcctgtaaa
    35041 acgggccagc ccaacattga cttttaccga tattaaccgc gtcagcgaac tgctctctgt
    35101 tcccacgaaa gatgcctcac gagatagctt tcccggcgat cacggcatga tgctgcttat
    35161 tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg cccttattat
    35221 ttttgtggtt tttgcatttc ccagagtaat gattggcgca cactggttta ctgacatcat
    35281 tgtcggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga cgccattaag
    35341 tgatcgatta atcacctttt ttgacaaatc actaccggga aaaaacaaac atttccaaaa
    35401 caaataactc acagtaatta acatcatcag gcttattttt atagtgagga taatcctgat
    35461 gatgcgcacc gtgctttcat ccatcgcgcg caaaaatcat tctctaagta aatgaatgga
    35521 ttgcatgcgt ttcactcaat cgtactttaa ttgaccaatc ccgcttatta actttctgta
    35581 tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc aatcgggtaa
    35641 tctcgaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt ttgtgcgtta
    35701 gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag tctcgtgtcg
    35761 cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa caaacaacat
    35821 ggtcaaatct caaccgattt tgagatatat cttgcgcggg atccccgcga ttgcagtagc
    35881 ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc ctgagacacg
    35941 tgcagtgggt agtgaaacat catcactgca agcttctcag gatgaatttg aaaacctggt
    36001 tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga aaggcgtacg
    36061 ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt tctggttttg tacaacgtac
    36121 attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc aggaaatggg
    36181 taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc gtgccggttc
    36241 aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg cttccaccag
    36301 cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt acaacgaagc
    36361 acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg gctatcctga
    36421 cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat cagagaaaat
    36481 gatgtttccg cgtcttgatc caggctatag tccggtcatt gttatctttt aaatgttgtc
    36541 gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg gacgtaagct
    36601 cctgcttacc tgcattgttg caggcgtgat gattgcgata ctggtgagtt gccttcaatt
    36661 tttagtggcc tggcataagc acgaagtcaa atacgacaca ctgattatcg acgtacaaaa
    36721 gtatctcgat acctattttg ccgacctgaa atccactact gaccggcttc agccgctgac
    36781 cttagatacc tgccagcagg ctaaccccga actgaccgct cgcgcggcgt ttagcatgaa
    36841 tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc tgttcatctg cgactggtga
    36901 gatggacatt ccactcaatg aattgattcc ggcgctcgac attaataaaa acgtcgatat
    36961 ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca tctggtatcg
    37021 caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc tgacgccttc
    37081 tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta ttggcaatac
    37141 tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg acatgccggt
    37201 ccgtgaaact aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg atgactggac
    37261 atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg tcgttggcct
    37321 gctctgctat tacctgatga gcgtacgtat gcgccccggc agagaaatca tgaccgccat
    37381 caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaag ctttgcgagt
    37441 aacgggcctg gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa ttcccccgga
    37501 tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccgctgactc agcacctgtt
    37561 tgagttaatt gcccgcgatg ccgcagaatt agaaaaagtg ctgccagtag gcgtcaaatt
    37621 tggtattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag atatccagaa
    37681 actgctcact tccctacccg cacaccattt ccagattgtg ctggaaatta ccgagcgcga
    37741 tatgctgaaa gagcaagaag ccacacaact cttcgcctgg ctgcactcgg tcggcgtaga
    37801 aattgctatt gatgacttcg gcaccgggca cagcgcgctt atctatcttg agcgttttac
    37861 gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg aaacgatcac
    37921 ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgt ctcaatatgc tgacagttgc
    37981 tgaaggggtc gaaacgccgg aacaagcgcg atggctaagc gaacgcggcg ttaatttcat
    38041 gcaaggctac tggattagtc gcccgttacc gctggacgat tttgtccgct ggctgaagaa
    38101 accgtatacg ccgcagtggt aaggtgtgct tacgtccctt attattcata gtgaaagcat
    38161 gccggattgc ggctaatgat gagtaaaagg aaatccgttg cagatgattg tgcgcatact
    38221 gctgctgttt atcgctctgt tcacctttgg tgtgcaggcg caggctatca aggaaagcta
    38281 tgcctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt ttgattatgt
    38341 gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca gccctcggca ccttcgatac
    38401 tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc tgtacgacac
    38461 gctatttacg acttccgatg acgaaccagg cagttattac ccgctgattg ctgaaagcgc
    38521 acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgcgcg cccgttttca
    38581 tgatggttcg cccattactg cccgcgatgt agagtttact tttcaaaaat ttatgaccga
    38641 aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca ttgcgccgtt
    38701 aaccgtgcgc attgagttag ctaaacccgg caaagaagat atgttgagtc tgttttcgct
    38761 gccggtattt ccagaaaagt actggaagga tcacaaactt agcgacccgc tcgccacgcc
    38821 tccgcttgcc agtggtccgt accgcattac gtcctggaaa atggggcaaa atattgtcta
    38881 ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgcggac gctggaattt
    38941 cgacaccatt cgctacgatt attacctcga tgataatgtc gcctttgaag cgtttaaagc
    39001 aggtgccttt gatttgcgta tggaaaacga cgccaaaaac tgggccacgc gttataccgg
    39061 taaaaatttc gataaaaaat acatcatcaa agatgagcaa aagaacgaat cagcccagga
    39121 tacgcgctgg ctggcgttta atatccaacg tccagtattc agcgatcgcc gggtgcggga
    39181 agcaatcact ctcgcctttg actttgaatg gatgaacaag gcgttgtttt acaatgcctg
    39241 gagtcgcacg aacagttatt ttcagaatac cgaatacgcg gccagaaatt accccgacgc
    39301 cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag tcttcacaca
    39361 aatctaccag ccgccggtat ccaaaggcga tggctacgat cgtgacaacc tgttaaaagc
    39421 cgacaaactt ctcaacgaag cgggctgggt gctgaagggt cagcaacgcg ttaatgccac
    39481 aacgggtcag ccactcagct ttgaattatt gcttcccgca agcagcaata gtcagtgggt
    39541 attgccgttc cagcacagcc tgcaacggct gggtatcaac atggacattc gcaaggtgga
    39601 taactcgcaa atcactaacc gcatgcgcag tcgcgactat gacatgatgc cgcgcgtatg
    39661 gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg aatatatcaa
    39721 ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcgctga tcaaccaaat
    39781 tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac tggatcgcgt
    39841 attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc gtctcgcctg
    39901 gtgggataaa ttctcccagc cggccgtgcg ccccatctat agcctcggta tcgatacctg
    39961 gtggtatgac gtcaataaag cggccaaact gccgtccgcc agcaaacagg gagagtagat
    40021 gggcgcttac ctgattcgcc gtctgttgct ggtgatccca acattatggg cgattatcac
    40081 catcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgaccagg ccatcgccgc
    40141 cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg ttcgtgccag
    40201 ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaag taccgtggcg gacgcggatt
    40261 agatccagaa gtgatcgctg agatcactca tcgctacggt tttgataagc cgatccacga
    40321 acgttacttc aaaatgctct gggactacat ccgctttgat tttggcgata gcctgtttcg
    40381 cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg gtttccatca ccctcggatt
    40441 gtggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca aagctgttta
    40501 taatgggagc cgctttgacg tctggagtag cgcatttatc atcatcggct acgccattcc
    40561 ggcctttttg tttgccatcc tgctgattgt cttcttcgcg ggcggcagct atttcgacct
    40621 gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt atcagaaaat
    40681 caccgattat ctgtggcata tcacgctgcc ggtgctggcg acagtgattg gtggctttgc
    40741 ggcgctgacc atgctgacaa aaaactcatt ccttgatgaa gtgcgcaagc aatacgtggt
    40801 gaccgcgcgt gcgaaagggg taagtgaaaa aaatattctc tggaaacatg tgttccgcaa
    40861 cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt tttttaccgg
    40921 ctcgctgctg attgaggtga tgttttcact caatggtctg ggcttactgg gctacgaagc
    40981 gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca ccctgattgg
    41041 cctgctgctg aatattgtca gtgatatcag ctatacgctg gtcgatccgc gtatagattt
    41101 tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc gcgttttcgc
    41161 cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg tttgagtttg
    41221 tgttctgaac ttatcgccaa cgataaaccg ttgctggtac gttatgacgg cagttggtat
    41281 ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg gcccgctggc aagtcaggct
    41341 gattatcagg acccgtggct gaaacaacgg ctggaaaata acggctgggt actgtgggca
    41401 ccgattcgct ttggtgctac cagtatcaac tttgctaccg ataagccctt cccttctcca
    41461 ccctcccggc aaaactggct gggaacggat gccaacggcg gcgatgtgct ggcacgtatt
    41521 ctctatggca cgcggatctc ggttctgttt ggcctgatgc tgactctctg ttccagcgtg
    41581 atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga tctctggggg
    41641 caacgcttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat tttactttcc
    41701 agcgtcgtac agcctaactt ctggtggctg ctggcaatta ctgtcttgtt tggctggatg
    41761 agtctggtcg gcgtggtgcg ggcggagttt ttacgcaccc gtaatttcga ctacattcgt
    41821 gcggcgcagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat gttgcctaat
    41881 gccatggtcg cgaccctcac ctttttaccg tttattttat gtagttcgat aaccaccctg
    41941 acctcactcg atttcctcgg cttcggtctg ccgctcggtt caccgtcact cggtgaactg
    42001 ctgttacaag ggaaaaataa ccttcaggct ccgtggcttg ggatcaccgc cttcttgtcg
    42061 gtggcgatat tattgtcttt gctgatcttt attggtgaag ccgtccgcga cgcatttgat
    42121 cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaagatt tgtcggtggg
    42181 ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcactac agattgaggc
    42241 tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta ccgcgctgtc
    42301 aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata ttcgttttcg
    42361 tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg gtaataagat
    42421 cgccatgatt tttcaagagc cgatggtgtc gttaaatcca ttgcataccc tggaaaaaca
    42481 gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc gaggcgaaat
    42541 tcttaactgc cttgatcgcg ttggtatccg ccaggcggca aaacggctga cagattatcc
    42601 gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc tgttaacgcg
    42661 accggaatta ttaattgccg atgaaccgac caccgcactg gacgtctctg tccaggcgca
    42721 gattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc tgtttattac
    42781 tcataacctc agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc aaaacggtcg
    42841 ctgtgtcgag caaaataacg ccgctacgct atttgcctca cccactcatc cttacacaca
    42901 aaagctactc aacagtgaac cgtcaggcga cccggtgccg ttgccagaac ctgcctcaac
    42961 gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga ttttgaagcg
    43021 cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacgag cgggtgaaac
    43081 actgggttta gtgggcgagt ccggttccgg gaaaagtacg acgggactgg cgctgctgcg
    43141 actgattaat tctcagggca gcatcatctt tgacggtcag ccactgcaaa atttaaatcg
    43201 ccgccagctg ttacctattc gtcatcgcat tcaggtggta tttcaggatc caaactcctc
    43261 gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg ttcaccagcc
    43321 gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg aagtgggatt
    43381 agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc gacaacgtat
    43441 tgcgattgcc agggcattaa ttcttaagcc ctcgctgatc atacttgatg aaccgacatc
    43501 atcactcgac aaaacggtac aggcgcaaat attgacgcta ttgaaatcat tgcaacaaaa
    43561 gcatcaactg gcctatttgt ttatcagcca cgatttgcac gttgtccgcg cgttatgtca
    43621 tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg cgcgcgtgtt
    43681 tgccacaccg cagcaggagt atacgcgtca gctactggcg ttgagctgac gcttaaaaag
    43741 gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa atttttcaat cggcatgttg
    43801 cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac tccagcaccg
    43861 aacatggaac ttcgatgtcg cttaacgcct ggcttagttt atacatcacc gaccatgcat
    43921 tatcaccatc cgggctaagc aatttaagag ccaccaggct gttgtcaccg cacggtccat
    43981 tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga tagttctgcg
    44041 gcagtcgatg gacgattgag agttgtaatg aagtcactta ttttccccgg aagcacattc
    44101 acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct ttatacagat
    44161 cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaagt aatatctgcg
    44221 ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt
    44281 aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac atatatcggg
    44341 ggaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttagc ttcctcgttg
    44401 tgcaatagat caccgttttt tcggtcgact ggcgtacaga cagaagagaa tggagctgct
    44461 tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag
    44521 caatgcgcca acaattgccc ctatgccaaa acggaaggtt cctgccagcg aagatgccgt
    44581 tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg acaccatcga
    44641 cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca gccccagcag
    44701 cgcactgatg accatccacg ctgccataat aaattgtatc cacaaccccg agcggaacat
    44761 atttaacgcg ccaatgcggc gaacgaagcg gctgttaaaa atggtcatca cgaacaggaa
    44821 aacaatgttt agtgcaaagt aataaccaaa gttttccggc gcgacgtggt taatttcaat
    44881 gtagacaaac ggtccggcgc ttaagaacga gaacatcccg gcaaagctga aaccactggc
    44941 aagcatataa ctcaggacac gcttatgacg gaacagcgcc gcaaagttac caatagtggt
    45001 acgaatgtga aaaggctggc gacgctccgg tggtaaggtt tctttaatca ggaagaaaat
    45061 cattgccgaa gccagaatcg ccgctaatgc caggatccag aagatgtaat gccagctcag
    45121 ccacaccagc acccaaccgc cgacaatcgg tgccatcagc ggtgcaatag tggtcaccag
    45181 catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcgc gcatcagggc
    45241 gttaatgacc acgctggccg cagccgccgc cagcccgtgg aagaaacgca tcacaatcag
    45301 ctgatcgatg gtgtttgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc
    45361 gccgagcacc accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc
    45421 caacgcaaag cccagaatat aagtactgag ggtcatctgc gtactgcccg caggtacgcc
    45481 aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca gcggcatcaa
    45541 catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac
    45601 aacgggctcc tgaaagtcat ggaaaagtta gacgacgctg gcaatttctt cttcagttaa
    45661 cggacgatat tcaccggggg ctaaatcagc atccagcgta ataccgccaa tacgttcacg
    45721 atgcagctca accacgtggt tacccacggc ggcgaacatg cgtttcacct gatgataacg
    45781 cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttccagca ccgcaggctt
    45841 agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc
    45901 agctacaggt gattccagtg tcaccagata ggtcttctcg caatgatggc gcggagaagt
    45961 aatgcggtgc gaccactgac catcatcagt catcagcacc agaccggtgg tatcgatatc
    46021 cagacgtcct gcggcgtgca gtttccaggc taccggttca tccagaaaat agagcaccgt
    46081 tgggtgatca gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta
    46141 acgtggacca tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcaggaag
    46201 cagtttgaac gctgcattac gaacaatttc gccatcgacg gtgacacgat tgccgcggat
    46261 ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg
    46321 catgtgtgtg attttgcctg taaaaacgga ggtcgggcat tgacccgaaa atctgaactg
    46381 ttgtctgccc agtatagcgg tctaataaca tccctcaagg gaaaaagatc catggcatac
    46441 tattagcaga ataatctacc tacgcgagac catgattttt acactccgcc catatcagca
    46501 agaagccgtg gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt
    46561 gctgcccacc ggcgcaggta aaagcctggt gattgccgaa ctggcacggc tggcgcgtgg
    46621 tcgtgttctg gtgctggcac acgtcaaaga actggtggcg caaaaccatg caaagtatca
    46681 ggcgctgggg ctggaagccg atatttttgc cgctgggtta aagcgcaaag agagccacgg
    46741 taaagtggta tttggcagcg tgcagtcggt cgctcgtaat cttgatgcct ttcagggtga
    46801 attttcgctg ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta
    46861 tcagcaaatc ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac
    46921 tgccacgcct tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt
    46981 acgcggcaat gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat
    47041 gattaaacac ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga
    47101 tttcagccgc ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga
    47161 gctgaaaaaa cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga
    47221 aaagcgcaaa ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg
    47281 attactgcct gccgaagatg ctgcactgat tactggcgac acccccggcg ctgagcgcga
    47341 tgtgttaatt gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact
    47401 gaccaccgga tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc
    47461 agtgagtctt taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga
    47521 ttgtttaatt cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac
    47581 accgaaaggc aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc
    47641 caacaccttt tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg
    47701 ctgtcaggga tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg
    47761 ttttaaaaat tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga
    47821 atgtgacacg gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga
    47881 tgcgctggta ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga
    47941 atggttgaaa atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct
    48001 gcaaacgccc gcccagcgaa ctgccttcga gcagcttttt atccgcccgc atacgcgcac
    48061 accgggcatc cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact
    48121 gcgacatccg gattttgtcg tcgcccgcat gaaaggtcag tactggcagg tgcgtgaaaa
    48181 agtgttcgat tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt
    48241 ttgattgatg tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca
    48301 gatcacttac ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt
    48361 ttactatcaa cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg
    48421 ccgctaacaa gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc
    48481 tggatcacga caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctga
    48541 ccatcgttgt tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt
    48601 acaaaccgaa gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa
    48661 aaaccccgct ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta
    48721 ttttttgcag gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat
    48781 taattgccgc cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta
    48841 atggtcaagg tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca
    48901 aagttaatag tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta
    48961 cggtctgccg ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag
    49021 ctcacttcgc ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct
    49081 gcctgcagct gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct
    49141 ttatccagtt gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc
    49201 gctttcgcgt tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg
    49261 cgccctactc gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat
    49321 tcggttaaat cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg
    49381 ttgatgtcga gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc
    49441 agatactcca ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa
    49501 ggatatttcg ctaattcgtc acgcaggcgc ccggttgccg cgcggctaaa tgccagaaag
    49561 tcctcttcgc cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac
    49621 aagccgtaag ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg
    49681 acggtttctg tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca
    49741 cgcttgataa gctggtgcag ggcaatctgg ttgatatcca gactcatgat aaactctcct
    49801 ttaagaccgg gcggtattca accaccgccg gttaagcgac gcaataaaag atggcggtca
    49861 ttttacagaa ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg
    49921 ttgctacggt aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt
    49981 tcccgctaca gtgatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa
    50041 aaacataagg ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt
    50101 atcaacacca gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgcccgcgcc
    50161 ttacagtcct ctatcaacga agacaaagcg cactaaggga aacagataac tggttatggt
    50221 aactcatcgt cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt
    50281 tgcactgttc aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga
    50341 ctggccgaca acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag
    50401 cttcctggtg ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc
    50461 ccagaggctg atgaggtttt tgtccgtcat tctggcaacg gcgggaatga cgctattact
    50521 gatcgatagc gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcaact
    50581 ggttatcaac ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt
    50641 gccggttatt ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag
    50701 cctgacgcgt cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat
    50761 cgcctcgcat gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca
    50821 gcgcgctaac ctgccgcttt cgtacccgat gacggcgcga cgttttcttg agaagcatgg
    50881 tctgcttgat gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt
    50941 ttccgtacag tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt
    51001 gctgttgatt actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct
    51061 ggcaggtttt gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac
    51121 agacaacggc atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct
    51181 gtcgacccgt acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg
    51241 gttattctcg tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt
    51301 ctcgatgccg agcgtacgca cccaatccga cgagcagacc gccacgcagt ggatcaactg
    51361 gctgggccgc tacgcacaag aagataaccg ctggttctcg tgggtctctt tcaatggcac
    51421 taacattgac gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa
    51481 tgtcgatgac cagatcaacc gcgtgctcaa tgcactgcgt gattctggca aactggacaa
    51541 tacggttgtg attatcactg ccggtcgggg tattccgctg agcgaagacg aagaaacctt
    51601 tgactggtcc cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc
    51661 gcagcgtatt aatgcgctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct
    51721 gctacatgtc agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca
    51781 acgccgtcat tactgggtta ctgcagcgga taacgatacg ctggcaatta ccaccccgaa
    51841 aaagacgctg gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag
    51901 agtgaaagat gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg
    51961 ttttatcgct aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg
    52021 tggaaaacgg tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg
    52081 tcatggggtg tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaat cgcccaccgg
    52141 atatcaccgg aaagcaccgg ataagacagg gataacccgc taatcacttg attggcgggt
    52201 ttttttatgt tttttagtca ccgaacatca ccggatttat aagctatcag ccggattttt
    52261 tagttatacg tttagttata cgggctggcg tataactaaa gccgtataac taaaaccctg
    52321 ttttttcgtg tggaatcata gacatatggc gagaaaaaca cacccattaa ccacagtgca
    52381 gatcaaagca gccagaccag cgcaaaagga gtacaccctg caggacggcg gagggctttt
    52441 tctcctggtc aaaccgtctg gatcaaaact ctggagattt tcctactacc gaccatcgga
    52501 caaaaaaaga atattgctga gttttggatc gcttgaagat gtttccctgg ctgatgccag
    52561 aaaacgccgt agcgagtaca ggacgttaat cagtgccgga actgacccgc aggaccacga
    52621 gaggcaaaaa agagagacag aggcccgaag acaagggaac acgttcgaaa atgtggcggc
    52681 ggcatggtac caggtgaaaa tcagccagaa tctggccccc aacacgatta aagacatctg
    52741 gcgttcgctg gataaatatg tattcccgtt catcggcaac acgccaatag ataccctcac
    52801 cgcccgaagg ttcgttgaag tgcttacccc catcaaggag cgcggcaacc tggaaacact
    52861 caagcgggtt ttacagcgcg ttaatgaggt aatggattac gccgccaaca gtgggctgat
    52921 tgatgccaat ccggctatga atgtgcgtaa ggcgtttccc tcacctgtaa aaaaacatat
    52981 gccaacaatc cgccccgaac agctgcccga gcttatgcag gctttatcag tatcggcaac
    53041 agaacggcag accagattac tgattgaatg gcagttactg accgtaaccc gtcccgccga
    53101 agcgtcatca acgcggtggg atgaaatcaa cctggacgcg aagcaatgga cgatacctgc
    53161 cggacgcatg aagatgcgca gggatcacgt tatcccgctt tccggtcagg ctatggcggt
    53221 gctggaggcc atgaaaccaa tcagccacca ccgcaattac gttttcccaa gtctgaaaga
    53281 cccacagcag ccgatgaaca gccagacagc taacgcagca ttgcggcgta tgggattcgc
    53341 tggcgtgctg gtgtctcatg gattacgcgc catattcagc acagcagcga acgaggaagg
    53401 attcgagccg gacgtaatag aggcggcact tgcccacgtc gacaccaacg aagttagacg
    53461 ggcatacaac cggagcaact acatagaaaa acgcatcgtg ctgatgcgct ggtggggcga
    53521 atttgtcgag gctgcggcga cgggcgtaac cctcgccagt ggtaaaaggg gtatccgagc
    53581 cgtgtagctg tacagaaaac cagtaaaaac tacgaaaacc atgtaaaacc gtcgtataat
    53641 tgcatcaaat ttaacgacaa ggccgtgaaa catgaaaccg ttaagatgca aaaaaatatc
    53701 agatgcaatt gcgacgggct gcaactggcc ctgatggttc agcatgaatt ttggtcaacc
    53761 tacgatccgg aggacagaac gacggcccca tcaaaaaaac aggtagtaga ttttctggta
    53821 tcccgtggcg cttccagaaa tctggcggta agtattgata aggtcgtacg tccggcatct
    53881 atgaagatcg gaggcaggcc caaaaaatgg cggtaacaat cctggaagcg gcagaaatgc
    53941 cgcttttttt ataattccat ttcaaatcat caatataaaa aacggtgtat accgtttaaa
    54001 aacggtggga actgttttta cccatatccg atgatttacc gtatttgtca ccggaataca
    54061 ccggattcac aaggtaaatc acgatggaag caatcagaaa aattatcttt cgccaggaag
    54121 taaaaaaaat tatccacatc aaggcagaca gcacgctgca aagcatgatc aacgccggag
    54181 aatttccgca gggttttcgc gttggtttac gccgtcgcgg atggtatgag gatgatgtgt
    54241 tgtcctggca gaaagaacgc gaagaggaag cacgcggaac ggctgcttaa cgggtatcac
    54301 agatatgaca aacacgaaaa aagcggcccc gatatggagc cgcctttctg aacaattaac
    54361 ccgctgcgcc ttatgtgtat gtgatcccaa acataagcac ggggatgata gccgctatca
    54421 ggctggtggg caatgcaatc agtctggttc agttcgttgc catacctgca atgagcgctt
    54481 ttccctgtac tctttaagga attgctcaag ggcaaaagca catggcgcga atctttctga
    54541 ttcatgctct atctttctgc gccgtctttt ccgtgccggt gataatgttt tggtcaattc
    54601 tttatcggtc attgtgttgt cctgcatagc aatgcgccgt aatacctcac accacggcgc
    54661 tgatagtttt tatcctttgg gttctatgcc gcgttgctgt aattctttgc ggagaacgcg
    54721 tttaatccaa gtggctaaag atgtgtcgcc atcggcctta gctgcttctt ctagctgcgc
    54781 tctgaagtct tccggtaatc gcatttggta tggaggcgaa cgtttttcta atggtgttga
    54841 cacggtaatt acccttcatt actataccta catggtaatt accattgtac ttgccattac
    54901 acaaaaatca acgccccgca gtgctgggaa cacatacggg gcgtctaacc aacaacgtaa
    54961 actaggagcc gttatggttg ccgtaaatca tataccacac cttgtacaca cacaaacggc
    55021 ctttgtgtgg cgttttctgg ccctgaatat cggagaaaaa aaccagttga tcgccagtgt
    55081 cgaaaatagc tatgattccc gcgcacctca taaaacgggt gccgggattg gcgtcctggg
    55141 caaaactgaa gcgcataaca cgcgccccgc gtgttttttt gtgccgcata gtcacacctt
    55201 atcaatggtg ggctgtacgg gggcggagca atccgcgccg gtttcttcag tgtccggtta
    55261 cgccaaccct gtacagtccg ccaccagcga aattggcgtt tccggtggcg gttatcttaa
    55321 ccacactgag gaggctgcca acatggctac tatccctacc cttgtacatt ctcaaaccgc
    55381 ctttctctgg cgctttatca tctttggcgc gtcagaacat caaatcatcc acgtaaccgc
    55441 ctggacggaa cgcgaagcgc gtagccgttg cccgtccggt tgtgttgctg tattcgccgc
    55501 ccgtattcgt caggaggtgt gccatgtgca ataaaaccac accggacgca gcagccgccg
    55561 cgctcactac gctgatgcac gcgcttatcg atattgaatg cactgcagag cttgcgcagg
    55621 gagaagaaca gaaagaccgg acacagttcg ccctggaatg tatccgatac atcgcaacgc
    55681 ggtcgctgaa tgacgctaaa aatattcttg ttgctgattg tgaaaatggg gggggttatg
    55741 cgtgatgatc gttttaattc cctgaaacaa gaattttccg gcgttcctga tgatgcggct
    55801 gatgcgcttt cgtcaatatc tgaaattatg cgggtggctt ttttctttct ttgcactgat
    55861 gagcacagag atacagggct aaatattctt gatattgccg ctaactatgc tgatttcgtg
    55921 acagaagctg ttttaagaaa aacaacggac ggggattaat atgcgtgata tttaccacga
    55981 aacaatagac cgcgcattta gtgcccttgc ttacgctgaa ggtatgtacg aaatattgcg
    56041 catatggctt gaaacacttg gcgacaatga acgcgacaaa caaaaatcaa gaattgtcac
    56101 ggcattaata acgcttcttg agcctgtaat aaatgagttg caggaaatag agacattgca
    56161 cgacagatat aacgaacagc acaccggaga ataaaaataa tgaaacttaa atattctggc
    56221 ttaactgcca gtggcaacac tcaccctaaa tttacgcgcg gtgatattta ccgcgaccag
    56281 tacggcggca cggtaatgat taagggcgtg gcgggacggt gcgtaactta ccgccgtgaa
    56341 ggttacgaat atgattgcgt gatgcctgtt tatcagttcc ggcgtgattt ttctctggta
    56401 cagaccgcgc cgcataacgt gcccaccagc aacgccaggg cacgggcaaa catccagaag
    56461 ctgaaaacca tgattaacgg attcaggggc aagaaatgaa aagcgcaccg aacttaaaaa
    56521 aacagcctta cgacaagatg accgaagtca ttatttttgc gggtagtgat gcctgggcac
    56581 atgcgaaaca gtggcaggaa caggacgggc gactggctgg cgataatgtg cctcccgttg
    56641 tgctggctga tgatcaactg gatgaactgg cagacctgag aatcatcgac gaggggcgct
    56701 attgtgtccg gctgtacaag gcaggccaca tcaggccatc aaatattaat gccattgcgc
    56761 acaagctggc ggcggcgggt gtaactgatg cgaattatta ccccgaaggg atgcacagcc
    56821 agaagcggga gaactggcgc gaatatctgg aacgggagcg ggggaaaaag ccaggggaag
    56881 aaaaacacca ccagcgaaaa accacgctac caatgagcgt gggatctgcc ggatacgaca
    56941 cacaacttga ttacgtggtt aaggggatta ttccggcggt atcgctgtgc agcatatacg
    57001 gggcgagcgg ttcatataaa tcgttccttg cgggttcgtg ggcgtgtcac gtttccacgg
    57061 gccgccagtg gggaggccgc agggtggcgc atggcgctgt tctctatgtg gttggtgaag
    57121 gcggtatcgg cgttccgcgt cgtgtaaaag cctgggaggt tgtgcacggt gagcaggtga
    57181 aaaatctgta tctggtaaac cgtcccatct ttccggctgc cccgcttgat gttgaggaaa
    57241 tggttatcgc tgcccgtcag gtggaacggg aaacgggtaa accggttcga atgattattc
    57301 tggatacgct ggcgcgctgc tttggcggca atgatgaaaa tgattcccgt gatatggggg
    57361 cgtttatccg tgggtgtgac aaactgaaac ggcgcacagg ggcaacggtg ctggtggttc
    57421 accattccgg caaggatgaa acaaaggggg cgcgtggttc cagtgcattt cgtgcatcac
    57481 tggacgccga ataccggata cgcagggagg acgcaggcag cgaagcgctg gttatctcat
    57541 gtaccaaaat gaaggacgcg gaggaactca aagaagcagc atatgactta cgcgtggtgg
    57601 agctttttac cgacgctgac ggggagttaa tcacgtcgct ggtggtggtg gataagccgc
    57661 gccctcccgt tgaactggag cgcatcgagg aggcagggaa caagacggag aatcacgccg
    57721 cgctatggca gtgcatcaga acacgaacag cacacgggga ggcgtgcact atcgcccttc
    57781 tccgcgacga tatgaaaaag ctggggtatg agatgaagca cttccggcgc tggctgtaca
    57841 agctggaaaa tgatggcgta atcgttattg acggtgatga cgtgcgcccg ctgtaaaaag
    57901 tggggagtaa aagtgggggg agtggggaat ttaacaaaat tgaaacataa tta
//
LOCUS       DAZFRJ010000008        57794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000008 DAZFRJ010000000
VERSION     DAZFRJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.008"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..171
                     /locus_tag="ACHKUN_000438"
     CDS             <1..171
                     /locus_tag="ACHKUN_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6658758.1"
                     /translation="YCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKIVVKYNNWI
                     TKDNGEFELAVH"
     gene            201..701
                     /locus_tag="ACHKUN_000439"
     CDS             201..701
                     /locus_tag="ACHKUN_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO6658759.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            793..3477
                     /locus_tag="ACHKUN_000440"
     CDS             793..3477
                     /locus_tag="ACHKUN_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO6658760.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3474..4548
                     /locus_tag="ACHKUN_000441"
                     /pseudo
     CDS             3474..4548
                     /locus_tag="ACHKUN_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(4636..5934)
                     /gene="exuT"
                     /locus_tag="ACHKUN_000442"
     CDS             complement(4636..5934)
                     /gene="exuT"
                     /locus_tag="ACHKUN_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO6658761.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6417..7829
                     /gene="uxaC"
                     /locus_tag="ACHKUN_000443"
     CDS             6417..7829
                     /gene="uxaC"
                     /locus_tag="ACHKUN_000443"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO6658762.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7844..9331
                     /gene="uxaA"
                     /locus_tag="ACHKUN_000444"
     CDS             7844..9331
                     /gene="uxaA"
                     /locus_tag="ACHKUN_000444"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO6658763.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9414..9965
                     /gene="ygjV"
                     /locus_tag="ACHKUN_000445"
     CDS             9414..9965
                     /gene="ygjV"
                     /locus_tag="ACHKUN_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO6658764.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9970..11214)
                     /gene="sstT"
                     /locus_tag="ACHKUN_000446"
     CDS             complement(9970..11214)
                     /gene="sstT"
                     /locus_tag="ACHKUN_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO6658765.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11613..12578)
                     /gene="alx"
                     /locus_tag="ACHKUN_000447"
     CDS             complement(11613..12578)
                     /gene="alx"
                     /locus_tag="ACHKUN_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO6658766.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(12861..13847)
                     /gene="ygjR"
                     /locus_tag="ACHKUN_000448"
     CDS             complement(12861..13847)
                     /gene="ygjR"
                     /locus_tag="ACHKUN_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6658767.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(13926..14605)
                     /gene="ygjQ"
                     /locus_tag="ACHKUN_000449"
                     /pseudo
     CDS             complement(13926..14605)
                     /gene="ygjQ"
                     /locus_tag="ACHKUN_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(14682..15185)
                     /gene="ygjP"
                     /locus_tag="ACHKUN_000450"
     CDS             complement(14682..15185)
                     /gene="ygjP"
                     /locus_tag="ACHKUN_000450"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO6658768.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15270..16406
                     /gene="rlmG"
                     /locus_tag="ACHKUN_000451"
     CDS             15270..16406
                     /gene="rlmG"
                     /locus_tag="ACHKUN_000451"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO6658769.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            16597..16680
                     /locus_tag="ACHKUN_000452"
                     /pseudo
     CDS             16597..16680
                     /locus_tag="ACHKUN_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            16677..17093
                     /gene="higA"
                     /locus_tag="ACHKUN_000453"
     CDS             16677..17093
                     /gene="higA"
                     /locus_tag="ACHKUN_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO6658770.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17138..19156)
                     /gene="fadH"
                     /locus_tag="ACHKUN_000454"
     CDS             complement(17138..19156)
                     /gene="fadH"
                     /locus_tag="ACHKUN_000454"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO6658771.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19482..21833)
                     /gene="ygjK"
                     /locus_tag="ACHKUN_000455"
                     /pseudo
     CDS             complement(19482..21833)
                     /gene="ygjK"
                     /locus_tag="ACHKUN_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(21850..22920)
                     /gene="ygjJ"
                     /locus_tag="ACHKUN_000456"
     CDS             complement(21850..22920)
                     /gene="ygjJ"
                     /locus_tag="ACHKUN_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO6658772.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23054..24487)
                     /gene="ygjI"
                     /locus_tag="ACHKUN_000457"
     CDS             complement(23054..24487)
                     /gene="ygjI"
                     /locus_tag="ACHKUN_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6658773.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(24550..24999)
                     /gene="ebgC"
                     /locus_tag="ACHKUN_000458"
     CDS             complement(24550..24999)
                     /gene="ebgC"
                     /locus_tag="ACHKUN_000458"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO6658774.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24996..28088)
                     /gene="ebgA"
                     /locus_tag="ACHKUN_000459"
     CDS             complement(24996..28088)
                     /gene="ebgA"
                     /locus_tag="ACHKUN_000459"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO6658775.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28272..29255)
                     /gene="ebgR"
                     /locus_tag="ACHKUN_000460"
     CDS             complement(28272..29255)
                     /gene="ebgR"
                     /locus_tag="ACHKUN_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO6658776.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29474..29806
                     /gene="ygjH"
                     /locus_tag="ACHKUN_000461"
     CDS             29474..29806
                     /gene="ygjH"
                     /locus_tag="ACHKUN_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO6658777.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(29848..31227)
                     /gene="ygjG"
                     /locus_tag="ACHKUN_000462"
     CDS             complement(29848..31227)
                     /gene="ygjG"
                     /locus_tag="ACHKUN_000462"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO6658778.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            31645..33165
                     /gene="aer"
                     /locus_tag="ACHKUN_000463"
     CDS             31645..33165
                     /gene="aer"
                     /locus_tag="ACHKUN_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO6658779.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33319..33942)
                     /gene="nfeR"
                     /locus_tag="ACHKUN_000464"
                     /gene_synonym="yqjI"
     CDS             complement(33319..33942)
                     /gene="nfeR"
                     /locus_tag="ACHKUN_000464"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO6658780.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34219..34983
                     /gene="nfeF"
                     /locus_tag="ACHKUN_000465"
                     /gene_synonym="yqjH"
     CDS             34219..34983
                     /gene="nfeF"
                     /locus_tag="ACHKUN_000465"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO6658781.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35037..35112)
                     /locus_tag="ACHKUN_000466"
     tRNA            complement(35037..35112)
                     /locus_tag="ACHKUN_000466"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35077..35079),aa:Ile,seq:cat)
     gene            35237..35743
                     /gene="mug"
                     /locus_tag="ACHKUN_000467"
     CDS             35237..35743
                     /gene="mug"
                     /locus_tag="ACHKUN_000467"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO6658782.1"
                     /translation="MVEDILALGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(35822..37663)
                     /gene="rpoD"
                     /locus_tag="ACHKUN_000468"
     CDS             complement(35822..37663)
                     /gene="rpoD"
                     /locus_tag="ACHKUN_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO6658783.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(37858..39603)
                     /gene="dnaG"
                     /locus_tag="ACHKUN_000469"
     CDS             complement(37858..39603)
                     /gene="dnaG"
                     /locus_tag="ACHKUN_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO6658784.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            complement(39714..39929)
                     /gene="rpsU"
                     /locus_tag="ACHKUN_000470"
     CDS             complement(39714..39929)
                     /gene="rpsU"
                     /locus_tag="ACHKUN_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO6658785.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40167..41180
                     /gene="tsaD"
                     /locus_tag="ACHKUN_000471"
     CDS             40167..41180
                     /gene="tsaD"
                     /locus_tag="ACHKUN_000471"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO6658786.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41223..42686)
                     /gene="ttdT"
                     /locus_tag="ACHKUN_000472"
     CDS             complement(41223..42686)
                     /gene="ttdT"
                     /locus_tag="ACHKUN_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO6658787.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(42734..43339)
                     /gene="ttdB"
                     /locus_tag="ACHKUN_000473"
     CDS             complement(42734..43339)
                     /gene="ttdB"
                     /locus_tag="ACHKUN_000473"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO6658788.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43336..44247)
                     /gene="ttdA"
                     /locus_tag="ACHKUN_000474"
     CDS             complement(43336..44247)
                     /gene="ttdA"
                     /locus_tag="ACHKUN_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO6658789.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            44454..45386
                     /gene="ttdR"
                     /locus_tag="ACHKUN_000475"
                     /gene_synonym="dan"
     CDS             44454..45386
                     /gene="ttdR"
                     /locus_tag="ACHKUN_000475"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO6658790.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(45399..46016)
                     /gene="plsY"
                     /locus_tag="ACHKUN_000476"
     CDS             complement(45399..46016)
                     /gene="plsY"
                     /locus_tag="ACHKUN_000476"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO6658791.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46121..46489
                     /gene="folB"
                     /locus_tag="ACHKUN_000477"
     CDS             46121..46489
                     /gene="folB"
                     /locus_tag="ACHKUN_000477"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO6658792.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            46579..47400
                     /gene="bacA"
                     /locus_tag="ACHKUN_000478"
     CDS             46579..47400
                     /gene="bacA"
                     /locus_tag="ACHKUN_000478"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6658793.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(47563..48801)
                     /gene="cca"
                     /locus_tag="ACHKUN_000479"
     CDS             complement(47563..48801)
                     /gene="cca"
                     /locus_tag="ACHKUN_000479"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO6658794.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(48865..49485)
                     /gene="ygiM"
                     /locus_tag="ACHKUN_000480"
     CDS             complement(48865..49485)
                     /gene="ygiM"
                     /locus_tag="ACHKUN_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO6658795.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            49727..51028
                     /gene="ygiF"
                     /locus_tag="ACHKUN_000481"
     CDS             49727..51028
                     /gene="ygiF"
                     /locus_tag="ACHKUN_000481"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO6658796.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51051..53891
                     /gene="glnE"
                     /locus_tag="ACHKUN_000482"
     CDS             51051..53891
                     /gene="glnE"
                     /locus_tag="ACHKUN_000482"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO6658797.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            53939..55372
                     /gene="hldE"
                     /locus_tag="ACHKUN_000483"
     CDS             53939..55372
                     /gene="hldE"
                     /locus_tag="ACHKUN_000483"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO6658798.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(55492..55551)
                     /gene="ibsE"
                     /locus_tag="ACHKUN_000484"
     CDS             complement(55492..55551)
                     /gene="ibsE"
                     /locus_tag="ACHKUN_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO6658799.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(55868..55927)
                     /locus_tag="ACHKUN_000485"
     CDS             complement(55868..55927)
                     /locus_tag="ACHKUN_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6658800.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(56166..57794)
                     /gene="yqiK"
                     /locus_tag="ACHKUN_000486"
                     /pseudo
     CDS             complement(56166..57794)
                     /gene="yqiK"
                     /locus_tag="ACHKUN_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
BASE COUNT        13789 a        14795 c        15458 g        13752 t
ORIGIN      
        1 tattgtaaaa ataatagcaa agaaaattgt aagatacaga ccgtaaataa aaacatcttc
       61 cctggtcagg aaagaaatct tgaaagcata tctggctacg acaaaatcgt tgtcaaatat
      121 aataactgga tcaccaaaga taatggcgag tttgaactgg cggtccatta attatcattg
      181 taataaacga aggaatagta atgaaaaagg tatttgcaaa atctcttctg gtcgcagcga
      241 tgttttctgt tgcaggctcc gcattggcag tacaaaagga tattactgta acggccaacg
      301 ttgatgccgc tctggatatg acacagaccg ataacaccgc gttgccgaaa gcagtagaaa
      361 tgcaatatct gccggggcag ggtctgcaat cttaccagtt gatgaccaaa atctggtcta
      421 acgacgttac caaagatgta aaaatgcagt tagtttcccc ggcgcaactg gttcaaagcc
      481 tggatgctag caagattgtt ccgctgacag taacctgggg cggtgaagaa attaaagctg
      541 atgcggcaac cacttttacc gcaaccaaaa tctttgcttc cgatgcgtta actaacggtt
      601 ctctggctaa aaacctgatg tttgctcaaa tcaccaaagg tgttctggag acaggcatct
      661 accgtggtgt agtaagcatt tatctgtctc aggatatcta atccagataa cagaattaac
      721 acgtaacaaa gggaggggta gccctccctt attttaactg ttttagtatt tgttatggat
      781 ttcaggattg ccatggataa aaaattactg gctcttttga tcctggcgag tctcagcccg
      841 gcagaggcga cattaaccaa aattcccgca gggtttgagg ttattgctca gggacagcag
      901 gagtatatcg aggtttattt ttcagggaaa agtctcggta aatattatgc aatggttaat
      961 cttgataccg taacatttct tgatccagca agtctatata acaagctgga actggatgta
     1021 gacgatcaga aaatcgcgca tatagtgaaa gaaaaattat cgcagccgct agctcgccac
     1081 ggtgaattgg cttgcggtta tgtacgtact gactcagggt gtggttttct gaataccgat
     1141 acgctggaaa taatctataa tgatgaagaa agttcggcaa cgttgtttat taatccgcaa
     1201 tggaattcag ctttcgatgc gaagtcatta tatttaaatc cagacaaaaa tactgttaat
     1261 gcttttatac atcagcaaga catcaatgtt ctggcacagg atgattacca atcgttgtct
     1321 attcagggaa acggtgcgct gggaataaca gaaaatagtt atattggtgc acactggaat
     1381 ttcaacggtt atgatgcaga tgatgtcagt gacagtaatg ctgacatcag cgatctctat
     1441 tatcgttatg attttttacg tcgttattat gtgcaggcgg gtcgcatgga caaccgcaca
     1501 ctatttaatg cacaaggcgg gaactttacc tttaactttc tgccactcgg tgcaattgat
     1561 gggatgcgta tcgggtcgac actgagctat ttaaaccagg cgcaaagcca gcaaggaacc
     1621 ccagtaatgg ttctgctttc gcgcaattct cgtgttgacg cttatcgtaa tgagcaactt
     1681 ttgggatcgt tttatctcaa tagtggttcg caatttattg ataccagttc ctttccgcca
     1741 ggcagctaca gcgtggcatt aaaagtctac gaaaataacc aactcacccg caccgagctt
     1801 gtgccgttta ccaaaaccgg cggtctgact gacggaaatg cgcaatggtt cttacaggca
     1861 ggtaaaacta catcacaggt ttctgatgat gaaagttcag cttatcagct aggagtacgc
     1921 ctgccattac atccgcaata tgagctctac gcagggctgg cgaatgccga tgatgtgagt
     1981 gctttcgagt taggtaataa ctggacggca gatttaggcg gggtggggaa tcttgcaatc
     2041 agcgccagcg tgttccgtaa cgatgacggc ggcaaaggtg atatgcaaca ggccaactgg
     2101 agtaatccgg gatggccgac gttgggcttt tatcggacca actctgacgg cgatgcttgt
     2161 acaaccgaca gcagagagag ctataacgcc ttaagctgtt atgaaagtat ttccgcgacg
     2221 gtttcacaga attttgtcgg ctggaatatg atgctgggtt atacccgcac acaaaataac
     2281 actgatgata gtttgcgttg ggataaacag cagagctttg aaaataacta tcttcgccag
     2341 acaacagcgc aaagtatctc tgaaactgta caacttagcg cttcccgcgc ttttgtgatg
     2401 cgtgactgga ttttgagtac gtcagtcggt gttttccatc gtaatgacaa cggtggcgat
     2461 aacgacgaca acggcttgta cttatcgttt tcgttatctg acacgccaac gatggatagc
     2521 aataacaaca gccattcaac aaatgtgtct acggattatc gttatagcga acaggatggc
     2581 gatcaaacgt catggcagtt atcgcatacc ttttataacg attcattcag ccataaagaa
     2641 cttggcgtaa ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt
     2701 tgggatgggc aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat
     2761 catgatcatc tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat
     2821 ggcgttaatt tgggagccag tggtacagac ggtttgctgg gtgcggtact ggtggatgtg
     2881 aaaggcttct ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca
     2941 ggcagccgaa ctttgcagct tggtcaaagt gacagtgtgt tgttccctta tcctggattt
     3001 cagtctggtt ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac
     3061 atcattaacg gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc
     3121 gaagtgtctg ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg
     3181 aaaaaattcc cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga
     3241 tttacacttg aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc
     3301 cttaagtgtc cactgagtgt tgtaaagaaa cgcgccagca ttcgttacag cggagatgtt
     3361 acttgtagtg tggtgactta ttcacaatta ccggagtcga ttcaggttca ggcacagttg
     3421 aaacagccta aattacgtgg aaacgttcag acggcgcaaa gggaggttgc accatgagaa
     3481 atcgattgat tgcggcgata ttgggcttgt ttggttcagg cagctcctga cgtgaccagt
     3541 gaaattacgt atgatttggc atctggtaga gcggattatt acttctggaa ggatgaagcg
     3601 tcggcaggaa ataatggata tatgtggtat gaatgttcgt atcctgacct ccaacaaacc
     3661 tgtacagcta atggaaatat atcgacagta caaatctatt taactgaaca acgcagtggg
     3721 atgcgttggc cggtaaaact caaaggattt aaaacagcca ttgtaagtag cgatgaagcg
     3781 ccgccaggat gcaagggggg caaagggctt cagacgaatc ttaaggattc taatagatct
     3841 tcatgtacag aagatggtca acattattat atatacgata caaagtttct tacgctctac
     3901 cttgagcaga cagagatgaa gaatttgccg attggtggcg tctggaaggg gaaagttaaa
     3961 ttacattcga acagcccggc ccaggactat ttcgcaaata ttaccctgaa tacgctcgac
     4021 cccaaccata ttgacgtgtt cttcccggag ttcgcccacg ccacgccaag ggtgcagtta
     4081 gacttgcatc caacaggaag cgttaacggc agcaactacg cgcaagatct gaccatgttg
     4141 gacatgtgcc tgtacgatgg ttttaacggt aatgccatca gttatgaaat catgctcaaa
     4201 gatgaagggc gaccagctgc agggcgcaga gacggttact tctctatcta tcgtcaggga
     4261 gggaccacca ccgacgaggg agaacgcatt gattaccggg tcaaaatgta caacccggaa
     4321 accggtgggc aaattgatgt gcgcaataat gaaaatatgg tctggaacag cattaacctg
     4381 aaacgtgtgc gtccggtggt actgcccggt attcgctatg ccgtgatgtg tgtgccgaca
     4441 cccttgactc tggcagtcga taaattcagc gtgatggata aacaggccgg gtactacatg
     4501 ggcaaattgt cagtaatctt tacgccttcc ttgccaacca tcaattaacc ctgcaaaccg
     4561 gacaaacgct tttttctata taaacgacga agccgtctga taaagcggct tcgtcattac
     4621 aacttaagtt aaacgttaat gttgcggtgc gggatcgtta tgtgtttcct gcgccacctc
     4681 aatcgccggt ttgttctgca acacggtcca gataaccagc gcacctaaca ggtcgaacac
     4741 tgccagaact gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac cgacaaccag
     4801 cgcaaacagc gtacttgcca gccatgcgga catcccggtt aaaccgttcg ccgtcgccac
     4861 ttcgttacga ccgaacacgt cagaagagag cgtaatcagc gcgccagaca gtgcctggtg
     4921 ggcaaaacca ccgatacaca gcagcataat tgcgacatac gggttggtga acagaccgat
     4981 catacccggg ccaatcatca gcacagcacc cagcgttacg accatcttac gggaaacgat
     5041 caggttcaca ccaaaccaac gctggaacag cggcggcagg taaccaccga ggatacaacc
     5101 gaggtcagca aacagcatcg gcatccaggc gaacatcgcg atctctttca ggttaaagcc
     5161 gtaaacttta aacatgaaca gcgggatcca cgcgttaaaa gtaccccagg ccggttctgc
     5221 caggaaacgc ggcagcgcga taccccagaa ctgacggtta cgcaggatct gaccaacgga
     5281 cattttcttc gccgtgctca cctggtgctg ggcttcctga ccattaataa tatagtcgcg
     5341 ttcttcatcg gtcagatgct tctggtcgcg cggatgttta tagaaaatca gccatgccat
     5401 cgcccagata aagctcaatg caccggagat gataaatgcc atctgccagc tgtgcattac
     5461 gattgcccat accaccagcg gcggcgcaat catcgcacca atcgaagaac ctacgttaaa
     5521 gtaacctacc gcgatggaac gctctttcgc cgggaaccat tcggagctgg ctttcagacc
     5581 cgccgggatc atcgctgctt ccgcggcacc gactgcacca cgagcaatcg ccaggccacc
     5641 ccagctacca gccagcgcgg ttgcaccaca gaacacggcc cacagcacag caaacattgc
     5701 ataaccgatt ttcgtaccca gcacatccag tacataacct gctaccggtt gcatgaccgt
     5761 ataagcagca gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc
     5821 ttccatcaga gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt
     5881 gccgagcgtc accagtgcga tcatatacca acgtaaccct ttaattttac gcatgtaaaa
     5941 cctcatcgtt ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat
     6001 gttagttacg accgggtaac cgaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt
     6061 ttcgtgctaa ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg
     6121 gcaatttgca ttccgtcgac ttatgatttg cgacggcaga aagataactt gtcatacaac
     6181 tttaaaaggt gagagccatc acaaatgtgc gaatatttgt agggacatta cctgacgaca
     6241 gcgaggccag tactggcgcg gcctgcagag agatttacca ctttgagagt aattttttta
     6301 actacgttta ttgatctaac tcacgaaaat atcttcggac tctggaaatt ggtgtgataa
     6361 ctttgtcagc atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga
     6421 ctccgtttat gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg
     6481 actacgcaaa agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg
     6541 cggaagacta tcgttttaaa aacctgtatg acatctggct gaaaggcgat cactacaaat
     6601 ggcgcgctat gcgtaccaac ggtgtggccg agcgtctgtg taccggtgat gcgtctgacc
     6661 gtgaaaaatt tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc
     6721 actggacgca cctcgaactg cgtcgtccgt ttggtatcac tggcaaattg ctttctccgt
     6781 caactgccga tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg
     6841 cgcgcggcat catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg
     6901 attctctgga gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc
     6961 cgagctggcg tccggacaaa gccttcaaca tcgaacaggc gacctttaac gactacatgg
     7021 cgaagctggg cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgcct
     7081 tgaccaaacg tctggatcac ttcgccgctc acggctgtaa agtgtctgac catgcgctgg
     7141 acgtggtgat gtttgctgag gcgaacgaag cggaactgga cagcattctg gcgcgccgtc
     7201 tggctggcga aaccctgagc gagcacgaag tggcacagtt caaaactgcg gtactggtgt
     7261 tccttggtgc cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc
     7321 gtaataacaa cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca
     7381 acgaccgtcc gatggcagaa gagttgtcta agctgctgag caagcagaac gaagaaaacc
     7441 tgctgccgaa aaccattctg tactgcctga acccgcgcga taacgaagtg ctgggcacca
     7501 tgatcggtaa cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt
     7561 ggtttaacga tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtt
     7621 tgctgagccg ctttgtcggt atgctgactg acagccgtag cttcctgtca tacacccgtc
     7681 acgaatactt ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag
     7741 caccggcgga catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc
     7801 gtgactactt cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat
     7861 ccatgcgctg gataacgtcg cggtcgcttt agcggatttg gctgaaggca cagaagtcag
     7921 tgtcgataac cagactgtta cgctgcgcca ggatgttgct cgtggacata aatttgcgtt
     7981 aacggatatc gcaaaagggg ccaatgtcat caaatatggc ctgccgattg gttatgcatt
     8041 ggcggatatt gcggcggggg aacacgtcca cgcccacaat acgcgcacga atctgagcga
     8101 tctggatcag tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcg
     8161 tgaagtgcag atctatcgtc gcgctaacgg cgatgtcggg gtgcgtaacg agctgtggat
     8221 cctgccaacc gtgggctgtg tcaacggcat cgcgcggcaa atccagaacc gtttcctgaa
     8281 agagaccaac aacgccgaag gtaccgacgg cgtgttcctc ttcagccaca cctacggctg
     8341 ctcacaactg ggcgacgatc acatcaacac ccgcaccatg ctgcaaaaca tggtgcgcca
     8401 cccgaacgcg ggcgcagtgc tggtgattgg tctgggctgt gaaaacaacc aggttgccgc
     8461 attccgtgaa acgttgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca
     8521 gcaggatgat gagatcgaag ccgggatcga gcatttgcat cagctgtata acgtgatgcg
     8581 caacgataaa cgcgagccag gcaaactcag cgaactgaag tttggtctgg agtgcggtgg
     8641 ttctgacggt ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt
     8701 gattgctaac ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca
     8761 gttgctgatg gaccattgcc gcgacgaagc aacgtttgaa aaactggtga ccatggtcaa
     8821 cgacttcaaa cagtacttta ttgcccatga ccagccgatc tatgaaaacc catcgccggg
     8881 gaacaaagcg ggcggtatca ccacgctgga agataaatcc cttggctgta cccagaaagc
     8941 gggttccagc gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccggggct
     9001 gaacttgtta agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg
     9061 ctgccatatg gtgctgttca gtactggtcg tggcacgcca tacggcggct ttgtaccgac
     9121 ggtgaaaatc gctaccaaca gtgaactggc ggcgaagaaa aaacactgga tcgactttga
     9181 cgcgggtcag ctgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacac
     9241 catcgttgag tttgccaacg gtaaacaaac ctgcaacgag cgtaacgact tccgcgaact
     9301 ggcgatcttc aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg
     9361 cgtttcataa tgtgaagcgc cgtttgcttt tccatacctg ataaggattt atcatgaccg
     9421 cgtattggct ggcccagggc gtgggtgtca tcgcctttct gattggtatc acaacatttt
     9481 tcaatcgtga cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg
     9541 tacatttttt tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc
     9601 gtacattgat taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga
     9661 ctggcggaat tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggta
     9721 cgattgtcag tacctgggcg ctgttccgct gtaaagggct caccatgcgc tgcgtgatgt
     9781 ggttttcaac gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacaa
     9841 tgattgaggg tagttttctg cttatgaatg gcttgaatat cattcgtttc tggcggatgc
     9901 agaaaagggg aattgatccg tttaaagtag agaaaacccc tcccgccgta gacgaaaggg
     9961 gttaaacaat taattacgca gggcgctatt tgccagacga tcgtcttccg cctggcaagc
    10021 tgccgcagtg aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa
    10081 tacgccgatg ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa
    10141 catattacag gccagcggga tcagcagcag agaacccccc gccacgccgg atgcgccaca
    10201 ggcacacaga gaagccacca cgctcaacag cagcgccgtg ggcagatcga ccgggatacc
    10261 cagcgtatta accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt
    10321 gatggtggca cccagcggaa tagaaacgga ataggtatcg cgatccagat tcagcttttc
    10381 acacagtgcc atattcaccg gaatgttcgc cgcagagctg cgggtgaaga aggcatacac
    10441 gccgctttcg cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca
    10501 caccagcaat gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag
    10561 cagttgcgcg tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag
    10621 accaaaaata ccaatcggtg cgaagcgaat gaccagtttc accataaagg tgacggcatt
    10681 cgacatatcg ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaaaccgag
    10741 gccaattgcc cacaccagga tcccgatgta gttacctttc agcagcgcgt cgatggggtt
    10801 ggaaaccatg ctcattacca gcccgcgcat cacttctaca atgcctgacg gtggcgaaat
    10861 atcacccgcg ctactggata agtgcagggt agaagggaag gcgaagctga aaacgactgc
    10921 ggccagagca gcagagaagg tacccagcag atagaggaac aaaatagggc ggatattggt
    10981 tttctgcccg tgctggtggt tagcaataga tgccatcacc agcatcaaca ccaggatggg
    11041 ggcaacggct ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc
    11101 gggttttgag atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac
    11161 caggctgcca tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg
    11221 atcctttcat tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg
    11281 taacaaagtg tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg
    11341 ctggatttta aagagaggtc atattttgca acttaatggt tacatttatt tgacataaaa
    11401 ataaagccaa cgtgcggctt ccagactgat gacaacatca aaaaaggact cggtcagtcc
    11461 cctcgccccg tttggggaga gggttagggt gaggggaaca ggccagcatc ggtgcgaaaa
    11521 attaccctca ccccgaccct ctccctggaa gggagaggga gaaaacggtg ctcatgttaa
    11581 tgacagggta cacggcttag gcagattaaa aattatccac cccgctgctt atcatgccga
    11641 taattcaccc aggcgttgat aataaacgtc atcaccagaa tgccaaacac cacgcccagc
    11701 gagacggcga ttggaatatg gtagaagtcg acaatcagca tcttgatacc gataaacacc
    11761 agaatcaccg ccaggccata tttgagcatc gagaaacgct ctgctacgcc cgccagcagg
    11821 aaatacatcg cacgcaggcc gaggattgca aacaggtttg aggtcagcac aataaacgga
    11881 tcagtggtca cggcgaagat agccggaatg ctatccacgg caaaaatcac gtcgctcaat
    11941 tccaccagaa tcagtaccag catcagcggt gtggcgtaca gcaatccatt cttacgcaca
    12001 aagaaatgct cgttgtcgat ggtgtcggtc atgcgcaaat gaccgcgtag ccagcgcacc
    12061 agcggcttgt cgccaatgcc tgattcatct tcatgggcga gcgccatctt cacgccggta
    12121 aacagcagga aggcaccgaa gatatacagg atccagtcga actgtgaaat cagccagctg
    12181 cctgtgaaga tcatgatggt acgcagaaca atcgccccga gcacgccata caccagcacg
    12241 cggcgttgta atgccgccgg aacagagaaa tagctgaaca acatcagcca gacaaagacg
    12301 ttatcgaccg ccagcgattt ctcaatcaga tagcccgtga gaaaggccag tgcctgtgga
    12361 tcggcaacgg cgcgaccttc agtttgcacc agataccacc agaaagcggc gttaaacagt
    12421 aacgacagcg tcacccagac cagcgaccag gccgcagcct gtttcatggt catggcatgt
    12481 gccccacgac gcccctgcaa caacaggtcg atagccagca taatggcgac aacaacagcg
    12541 aatccgcccc atagcaacgg cgtgccgaca gtattcatag aagttcctta cacataaaaa
    12601 aacggccaac gtcggaagac gtcagccgct gctttttatg catagacctc gccttccggc
    12661 aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat tcctttcggg ttgcccggtg
    12721 accggatgtg gtttttcaca catcgtaatg acgatcgacc ggcaatgaag ttactcccct
    12781 ttgcaggtaa caaagtatga cagaccattc agccagtcaa tggtctgtta catacctttt
    12841 acactgtttt actttggcaa ttatagtttt acgctatctg ccggaaaaat caccccagtc
    12901 tggcggcgga tctcggtcag cagtttggcg gtgatgcgac tgaccgccag ccccggatga
    12961 tccaccagat gctcatccac cagggtagcg aacagctctg cttcgtagag catggtatta
    13021 atatgctgcg gctgggtgag atcctgcatt tggctgccac gcggcacgaa gcacactttc
    13081 tggcattcag acagtttttc aatcaccagc gatcctgctt cgccctgaat ctcgctcgcc
    13141 aggacagaat cactgacttt tgagtgctgc aaggtgacgc tgaaatcacc gtaatccatc
    13201 accaccacgc cgtgggcatc tacaccgctt gccagcaaac tggcggttgc ctgcacgctt
    13261 ttcggctcac caaataacgc caccgccgac gccaggcagt aaaagccgat atccataatt
    13321 gaaccgttag agaatgacgg attaaaggtg ttgggatttt caccgtccag gtaacgttga
    13381 tagcgcgagg aatactggca atagttgaaa aagactttac gcaatttgcc gactttcggc
    13441 agcgcctggc gcagcaaatg aaagttcggc aggcaggcgg ttttaaatgc ctcaaacagc
    13501 accacctgat tttcccgcgc acaggcaatg gcggcatcca cttccgccag attcgacgcc
    13561 agtggtttct cgcaaatcac atgaatttta tggctaagga aaagttgtgt ctgggaaaag
    13621 tgcagggcat tcgggctggc aatatacacc gcgtcaatgg catcgctttc cgccatcgct
    13681 tccagcgagg taaacagatg ctcgacagaa aaatcattgg cgaagtgctg agcctgctca
    13741 aggctgcggg aatatacggc ggttaacttg tatttaccgc tctcatgggc ggcctcgacg
    13801 aactggcgag tgatccagtt cgtaccaatc acagcgaaac gtatcataac gcgtggatac
    13861 tccataaagg gattctgtcg tcaatgtagc acgccttttt catcttcgac gtgcgcagat
    13921 acgcattatt cttttgccgg acagccatgc tcactaaacg gcccaatcat gaccgatggc
    13981 ccgagatatt tcggctggcg atggaggatt ttggcgtcaa tcacggcgct gacacgagcg
    14041 agtttctccc gcagttgagt ataaaaaccg tgcttcatat tgaggtcagg cgcgttaaaa
    14101 ccgatagcgt cgatgccgta ctgttttgcc agccagatgg cgcgttgatt atgaaattct
    14161 tgcgagataa tggtgatatg gttttcacca aaaacctttt tggctcgcac taccgaatcc
    14221 agcgttgaga atccggcata gtcgcagaag atcactttcg ccggtacgcc tttagcgatc
    14281 aagcctgcat acctgaggct tcatcgtaat ttttacgtcc gttatcgcca cttaccagta
    14341 gccatttcac ttttccggcg tggtacaacg ctgctgcggt atcaatacgt cgggtaaagt
    14401 agcgattacc cggcctggcc cccagcaata agcccacgtt acgcgccgga actgcgttga
    14461 catcgctcca ggtcagctgc ttgctggcgt tgaccataac gcgatcggca ataaaaatgg
    14521 tcgctcctgc tataagcaac aacaaacagc caatcttaag agtccggcga gaaaaacaaa
    14581 tacggagaag aaggcggggc tgcatggtcg ttatccttaa cgaaaggggg gaggtcgatt
    14641 ttaggcgcta aagaaagaaa agataacgcc tgacatcatt ttcagatttt gtcctgccca
    14701 agcgacaact gcgttagcca cagccgggtg tcgaactcaa gctggtggta ctgcggctcc
    14761 atatggcagc acaactggta aaacgctttg ttatgctctt tctccttcag gtgcgccagt
    14821 tcgtgcacca cgatcatacg taaaaacggt tccggcgcgt tgcgaaacac agtagcgacg
    14881 cggatctctg cttttgcttt cagcttgccg ccctgcacac gagaaacagc ggtatgtaac
    14941 ccgagcgcgt tttttagcac atggatctta ttgtcataca tcactttatt gatcggcggg
    15001 gcattgcgca gaaactgatt ttttagatcc ggagtatatt gccagagggc tttatcggtg
    15061 gcgtagtcgt gtgttcccgg atagcgtttt gccagcacat cgcccagacg ctgttcgtta
    15121 atcagcgttc gcacctggga aagtagctgc tcgggataac cctgaagata agtaagattg
    15181 ctcataaccg cccacgaaaa agagaaaaag ggtatactca cgcacccttt tcaggggcaa
    15241 cgtcgaaatt ttatcattca ggagggccga tgagccactt agacaacggt ttccgttcac
    15301 tgacactaca acgttttccg gcgacggatg acgttaaccc gctacaggcg tgggaagcgg
    15361 cggatgaata tttgctgcaa cagttggacg acacagaaat ccgcggcccg gtgttgatcc
    15421 tgaatgatgc ctttggtgcg ttaagctgcg cgctggcgga acataagccg tacagcatta
    15481 gcgactcata catcagtgaa ctggcgacgc gcgagaattt acgcctcaac gggattgatg
    15541 agtcgagcgt gaagtttctc gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc
    15601 tgatcaaagt gccgaaaaca ctggcattgc tggaacaaca actgcgtgcg ctgcgcaaag
    15661 tggtcacgtc ggatacacgt attattgccg gtgccaaagc ccgtgacatt cacacttcca
    15721 cgctggaact gttcgaaaaa gtgctcggcc cgaccaccac cacgctggca tggaaaaaag
    15781 cgcgcctgat taattgcacg ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta
    15841 gctggaagct ggaaggtact gactggacta tccataacca tgcgaatgtc ttctcccgca
    15901 ccgggcttga tatcggcgcg cgcttcttta tgcaacatct gccagagaat ctcgaagggg
    15961 agattgtcga tctcggttgc ggtaatggcg ttattggtct gacgctgctt gataaaaacc
    16021 cgcaggcgaa agtggtgttt gtcgatgaat cgccgatggc ggttgcttcc agccgtttga
    16081 acgttgaaac caacatgcca gaggcgttgg atcgcagcga gtttatgatc aacaacgcgc
    16141 tctccggcgt ggagcctttc cgctttaatg ctgtgctctg caacccgccg tttcaccaac
    16201 aacatgcgct gaccgataac gtcgcctggg agatgttcca ccatgcccgc cgctgcctga
    16261 aaatcaacgg cgagctgtat attgttgcca accgtcacct ggattacttc cataaactga
    16321 agaagatttt cggcaactgc accaccatcg ccacgaataa taaatttgtg gtgctgaaag
    16381 cagtgaagct ggggcgtcgt cggtaagatt gggagttacc tgccggatgc ggcgttaacg
    16441 ccttatccgg cctacgttca gcacgttact cccaggccgg ataagacgcg gcaagcgtcg
    16501 catccggcaa tgtctgccca gttctggaat cgcgcttcca taaaacaaaa aatccctgtc
    16561 accatcctct acattctctt gtttagcgtt gttcaacgtt tatgctacat acgtgaagtt
    16621 atgacgcata aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga
    16681 ttgcgattgc cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg
    16741 cgggtattca gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc
    16801 tcaacgatcc tgaaaacccc ttgctggatc tggtgtgtgc aaaaataacc gcgtgggaag
    16861 aatcagcgcc cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag
    16921 ccgtgattcg tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg
    16981 gcagtaaatc tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg
    17041 ctaaaaaatt ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg
    17101 tgccggatgc gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg
    17161 ttccctgggc aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta
    17221 aatgcacagt tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc
    17281 ctgcgcagat caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat
    17341 gcagcccgtc atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg
    17401 agagcagggt ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc
    17461 tggctttgcg ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg
    17521 ggcttaagcc accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg
    17581 cgatattctg gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa
    17641 tcccgccaca accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat
    17701 cgagataact caataccttc ggatgatcga tcccgtcgat aggcggaacg cgcggcacga
    17761 tcccactggc gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat
    17821 tgagtttcag cgtcacgccc gtcacttcga tcatccggtg gtaatagcgc agcgtttcgt
    17881 aaaactcctc tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt
    17941 gggcatcaaa caatgttaca tgatgcccac gcgccgccgc gttaatggca aacgccagcc
    18001 cagcaggtcc cgcaccgacc accgccagat tttttttctg cacggcagga aggattggca
    18061 ttttggtttc gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga
    18121 tctgatcgag gcaggcctga ttgcagccaa tgcaggtgtt gatctcatcg gctcgccccg
    18181 attgcgcttt tgacagcagt tccgcatcag caagaaacgg tcgtgccatc gataccatat
    18241 cggcatcgcc gcgcgagaga atgtcgtcgg caacctgcgg atcgttaatc cggttggtgg
    18301 tcaccagcgg cagcgaaacg tggcctttca gtttgcgcgt gacccaagta aatgcgccgc
    18361 gcggcacggg cgtggcaatg gtcggaatac gtgcttcatg ccagccaatg ccggtgttga
    18421 taatggtcgc gcccgccgct tcaatggcct gcgccagctc taccgtttcg gcaaaagtcc
    18481 cgccgtcttc gaccaggtcg agcatcgaca gtcggtagat aataatgaag tcgttgccga
    18541 cgcgttcgcg caccgcacgc actacttcta cggcaaatcg catccggttg cggtaatcgc
    18601 cgccccactg gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc
    18661 cttcggaccc cattacctct acaccgtcgt atcctgcctc ccgtgctagt tgcgcgcagt
    18721 gggcgaaatc gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac
    18781 ggttgatggg ggcctgcaat gcggaggaag cgaccagatg cggttggtag ctgtagcgcc
    18841 cggtatgcaa aatttgcagg gctattttgc cgccttcctg atgtaccgct tcggtaatgg
    18901 tgcgatggtg tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc
    18961 ctgttaaatc tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt
    19021 cggcataaaa cgctgccagc cgctcggcac cgtccgggta ttcctccagc ccggtgtgca
    19081 ttgagcccat caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca
    19141 gcaacgggta gctcataaaa ttgtccagta tgtaaaataa ttgttatgtg gtcggatgag
    19201 ttctaattta gccgtcgccg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat
    19261 tcaaaaagaa tacgcccggt ggtaacgccg gatgcgacgc ttgccgcgtt ttatccggcc
    19321 tgggggattg cacatgtagg gcggataagg cgttcacgcc gcatccgcca gtggcacggt
    19381 gcagatgccg gatgcgacgc ttgtcgcgtt ttatccggcc aggaggattg cacatgtagg
    19441 gcggataagg cgtttacgcc gcatccgcca gcagttgcaa attattgctt acggaaaaaa
    19501 tcgttataca acatatacaa atgcgcggca ctccaggaga aatttggtgc gccttgctgt
    19561 gcgccagtca gcgggttgta attctcctga attgggccat cggcggttaa tcctttggcg
    19621 tgacggaaga acgtatccgc cagtttcagg gcatcatcac gataaccgta acgctccatc
    19681 cctttcagac caaaccagaa ctgatccacc catacgcgcc cgcgccagta gatatcagcg
    19741 ccaaaagccg gattggttaa cgccgccgtt cccagcggga caaaggtgtt gaactcttta
    19801 ggatcgagca tcaccttcac caccgcgtcg gcgttggcct gcgttgccgc accgttaaac
    19861 agcggcgacc agccttccgg ccctttaccg cgctcgacaa tcggtttgcc cgcgcagccg
    19921 ttcgccagcg gtttatcttc aatacgcacg tcatagtaga actgggtagc cgggtcgaac
    19981 atacaggtgt ttatgtagtc tgcgagctgc tgtgccaact ggcgatagcg tttggcctct
    20041 tccggcttac cgaggatggt tgccatctcc gccagataat ggttatcgct gtacatatag
    20101 ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc gtcctgactg
    20161 cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccattagc gacatattta
    20221 tccaactgtt ctttgtcgat aaaaccaaag acggcggcat catcacgccc cgattcccac
    20281 gacgcagcga cctgtgccgg aatttccaga ctgtcgtact ggcctttctc caccacgcgg
    20341 gcgtagttgt tcagcccaga ctgcgtctct tctttgttgc ctttcttcac cgtaaacagc
    20401 atctcgccgc tctcagtgtt gtgggctttg tcgcgggtcg cgccatatta cggcacgccg
    20461 ttgccgttgt gatcgcggtt acgtaaccac cagtcgtgat aggccaccag tttcgggtac
    20521 atctctgcca accaggcttt atcctgggtg acgttgtaca cttccatcac cgaccaggcg
    20581 gcaaggctgg gtttggtatt acgttcgttc cagttgccgc catcgccacc acgctcgggg
    20641 ctaaggttcc acgctatcag gtcggggaca aagcccacat cctgcggacg cacgctatcg
    20701 ccaggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat gtccggattg
    20761 aaatgcgcca tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg attgccggag
    20821 aaccagcgcc cggtcaccga cggtgtaacg gtgttaaatt tcaccgcacc gcccggtgag
    20881 cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc
    20941 gcatccggat tggttaaccc tttcttcaga tactcttccc agcgttgctg cgaggcggtg
    21001 agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt gctaacttcc
    21061 tgagaggtca gcagatggga ataggtggta tagagcgtcg tcgaaccgtt gatatgtgcc
    21121 ttactggtaa agcgattgcc gttgatttca gtctgcaccg gcagggattt atgcacctga
    21181 tattccgatt cgccggaggt cagcagatcc caggtggcgc gcactttgcc aaaggtgact
    21241 ttcaggccat cacgggtggc gctgattttg cgctggtagt cagggtattc gccagcaatg
    21301 gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag ttcgccgtcc
    21361 cacaccagat ccagcggttt attgctggtg attttggttt ccagtagtga cgtgcgcggc
    21421 gtggcgaagc gcagagtcat ttcgacttgc acatcttttg ctgtcagttt ttgcaccagc
    21481 gcaccgggaa tactgtatgc ctccagcgta aagtcgactt tcttgccgtc ctgccagacg
    21541 gtcaggcggt cgaaattgct ggccataaag ttgatgtact cttccgtcag cagcgcaacg
    21601 cccggaaagc cgcccatggt gttagggccg tctggcaaca gatgaccatg ccaggctccg
    21661 agatcgaaaa acggattaaa acgctggtga tcgtcgtaat cgtaatcctt catgtactgc
    21721 ggcgcgccag tacggttaat cacgttttta taattgtcgg cgttagcggc atgggcggaa
    21781 aaacttatca gcagagcgca ggttactggc gttaaaatag tttttatttt catgaaatta
    21841 cagtccttat taccagtaga aatattgcat caggaagatt tggtcgctgg gatcgtgaga
    21901 ggcatttttc atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc cgccgtattc
    21961 gtaccagata ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg
    22021 tttggcgtag gtgtaagcgg tggagagata aacgcccttt aacgcttcat acatggcgct
    22081 caccatcatc ccactttcac ttcccggtac gtccagcccg tcggcgcgtc cggtatggtg
    22141 ccaggcgcga gcggcaaagt taggagttag caggcccgtg agatagagct gtcctgtgcc
    22201 gtcggtgatt tccagaccgt tcatccaggt gatgccttcg gcaaggtcat actggatgta
    22261 accgttcatc attgccggat aggtgtattt gtcgtcatag cgatcgtact tgccaaaatg
    22321 tagccaggct ttgctttcgt catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa
    22381 gttgccgctt aagttctgca ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac
    22441 gtagcccaga tcgggggtga agtcgcccca ccattgcagg tcatccagcg aggaatcctg
    22501 acgcagactg agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt
    22561 aattccgccc tcgaaaccac cccagctatg gtctggcacc caggcattgc cgtcgtggtc
    22621 ggcctgcacg gtccagcctt cgccgtaaat caggccgaac caggagccgt acttcatctc
    22681 caggccacct tcaatatagg taccgtcgct gtatttatgt ttattctcac cctctaaatc
    22741 catatatcca ccaaccccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt
    22801 aattgcaggt gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga
    22861 aaaggcgtag caaaacggca gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat
    22921 tgtgttaatc catgagctaa agaaaaaata cagcctttta tcgttgtaat tataaaaata
    22981 taaaggcaga aactattttc cctctcctca tccaggaaga gggaaaatgc ctgctcattt
    23041 tttattattc tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag
    23101 ccgttaatcc ctttatatat ttactgtatt tccaccacgc aaagccaagg aagataacaa
    23161 taccgccgac gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag
    23221 cgacaaaccc tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc
    23281 gggagcccat acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag
    23341 ccagcatgat gaataacggc ggaagcatgg acgctgcggc ggtcatatta ataatagtat
    23401 tcatcagatc ctgcacggta ttggaaccga gcatcgggat aatcatcagc gggatgacga
    23461 tcaggaactg gatccacgct gcgcgcgccg gaacgccgtt ttcgttcagt tcgacggttt
    23521 tcttaccaaa gatgccttcc gggatttcgg agaagaaaat tttcaccggc gttgcggtcc
    23581 acatcagcag ggaaccgaac atcgcggtaa aggacaccag accgacaaag cgattcatca
    23641 acgcttccgg tagaccaaaa tacgccgcca tgccgtggaa tacctgcacc gatccgacgg
    23701 taaatttcaa ctctttgctg ctgacgaaga cgttaatcag caccgaggag acggaataca
    23761 gtacgccgat aaaaatcccg gcgaggatga tcactttaac gaacgatttc gaaccgcctt
    23821 tgacgtcgtt aacgtacaca gcgacggact ccgcgccacc tgcggccata aagatccagg
    23881 tggtaacgcc gaggaacgcc cagttgaagt tcgggatcat cgcgtcaacg gtgatggggt
    23941 cagcaggctg tacgccgcca accagcgccg tacctgccag taaaatgtag gagagcgtta
    24001 acagcagcat cagcgttgaa gtgacggagg taattggccc caacattttc gccccgttgg
    24061 tggaaaccca ggtggagaag gcgaacagca ccatactgat aatggtggtg gcaaccggcg
    24121 tcataatata ttcgtatccg aggaaggcat acgaagcata agcaataacg cgcggcaaca
    24181 gtgaggtgaa aaagaacagg tttacgaacc agtaggtata ggcagtaata aatgcccaac
    24241 gtccgcccag cgaacttttt acccacgcgt agacaccggc ttctgagttt ttatttaacg
    24301 aaacaaattc tgcgatgatc agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa
    24361 acatcggtgc cgaggccagt ccaagctcaa tattattatt gataacgttg ttaaagctgt
    24421 aaacggcggc aaaagtcagc gagagcaagc cgaatttgcc gattgtatta cgtttggtat
    24481 cagacataac acatctccga aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg
    24541 ccgtagttgt tatttgttat ggaaataacc atcttcgatg gtgactttga gaaccacttt
    24601 tttgaccgcg ttattgcaga taaaccgata cgcttcatgg atatcgcaaa taacgatttg
    24661 cccttcgtgg acctcaacgg tttctccgca gccttttaaa tattcacggt cagtttcatc
    24721 acgataatat tccactacct gtaatgtctc tttcggtgca tattcaattt tttgctgccc
    24781 ttgcaggtag taatgcactt caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc
    24841 ggagtctgtc tccacgcggt aagtcaatga gtcaccgatg gagtgggcga cgccaggctg
    24901 aatgttgtcg atattttcaa tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta
    24961 aatctggcgg aactgttcta agttatcgat gatcctcatt gcttattctc gctgtgcaaa
    25021 ttcgtggaaa agaaccctgc gccgaactca tacgacgcca ggctttgcgc ggtagcttct
    25081 ccgccagaaa ccggcagcaa cgtaaagccg tagctgaagt cacggaacca gacgcgccag
    25141 gagtccagca cctcgctgcc ccaggagttg gagccgaggc caagtagctg gtggtcgagg
    25201 ttcagggtga tgtcatcact gcgctgcagc tcgttacagt gctgggcagc gtggatgttt
    25261 tcctgggtat agtgccaggc gctgaagtta attgggcgct gcggaaccac cagcagaccg
    25321 ttgccgtggc ggttagtcag tgccgtccag cggacatgct gacggttgcc gttgttctgc
    25381 gggaagggat agttctcgaa catggcatcg acggtgctgc gccagatatc gatgatgtta
    25441 gcctgctggc tgtcggcgta gttttcgccc ggtccacgac cgtaatacgc cacctgatcg
    25501 tattcgccgt taatccccat ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg
    25561 taacgctcgc cggaaagcgc cacgttaacc tggccatcgg cagcgatgcg ccagatgtag
    25621 gtgcagcgca tcccgaagtc aaacaccggc ggggcaataa ctgtgcggct gatgatcaac
    25681 acttcaccat cgctctgttc tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa
    25741 tgattcggtt gccacagccc ttcgttctcc tgcttgtggt tgtcgatcat tggcttgaag
    25801 aagttgatct ttggctcgcg ggtcagcagc gactcgccat tcacctgcca ggatgtcggt
    25861 ttgccactca tttttgagaa ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa
    25921 cgatcgtctt ccagcgtcag cggacgcgca ttatttggtg cgaaaggcac tggttgcgcg
    25981 gtgttttcct tcagcgggaa ctgataagtg gcgatagaat gcccggcttc gctgtagcgg
    26041 gtgcgggaat ctttggtcac cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc
    26101 agcgtgatct gcaagggggc ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc
    26161 gtcgcgagcg tttcaccttc gacgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg
    26221 gtaaaccaca gtttattttc gactttcagc tcgccgcgag tcagatccag cgcgtggatt
    26281 tttaccggcg cgataacctg tttgtactct ttcaggcccg gtcccggcgt ctgatcggaa
    26341 tagatcaaac catcaagaca gaagttatag ttgttgggat agtcgccgta gtcgccgccg
    26401 aatttatacc agacgttgcc gttgtcatcc tgcgcctgga tcccgtggtc gcaccactcc
    26461 caaacataat gtccctgaat acaatcgtgc ttatagaaga cgttctggta ctccgtcagc
    26521 ccgcctggtc cgttccccat cgcatgagca tattcacaga tgatgcgcgg cttcggatgc
    26581 gggtattcac caaactcatt catcagcggc acgcgggtgt acatggtgga aataatatcg
    26641 accacttcag catcgcgatc ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgccttc
    26701 gctgcgtggt acatcgcgcg gatgttacag ccatagccgg attcattgcc cagcgaccag
    26761 atgatgatcg acggatggtt tttctgcgcg tgaatatggc gaacaatgcg ctcgacgtag
    26821 actttttccc actgcggatc gtcggtaata cggctgatat cgccgacatt agcaaagccg
    26881 tgcgattcga cgtcggtttc cgccatcaca aacaagccgt agatatcaca cagttcgtaa
    26941 aaacgcggat cgttcgggta gtgagcggta cgcacggagt tgatattgtg ctgcttcatc
    27001 aactggagat ctttctcgac gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg
    27061 tcgtgacggt tgacgccgtg cagcatcaca taacggttat tgatccagaa cagaccgtcg
    27121 cgcactttga tatcacggaa gccaacgcgt tgtggcacca cttccagaac gttgccgttg
    27181 gcgtctttca gcgtcatgac cagatggtaa agataagggg attctgctga ccattgctgc
    27241 ggctgttcga cagtaaaagc aaagctggcg ctggtcagtt tttcaattgc caaatgatca
    27301 atggcgctgc tgtgcaccac gcgttcgcca tcaaacaagg tatattccag cgtcgtgacg
    27361 acaggggagg cggcgagatt ttccagcacc acttcgcagg aaagcgtggc atcgcaatag
    27421 gcttcgtcaa agtcggtacg cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc
    27481 agataaacat cgcggaagat ccccgctgac caccacatat cctggtcttc cacgtaggta
    27541 gagtccgccc actgcatcac gcgcacacac aacaggttgt cgccggtttt aaccatcgcg
    27601 ctgatgtcaa actctgcggt caggcgactg cccttgctga aacccacata ctgaccgtta
    27661 acatagactt caaaataggt ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc
    27721 cagccgtcgc tgagggtgaa aatacgttga taggcaccgg ttgggttatc gctggggaca
    27781 aacggcacat cgatggggaa cggaaaacct tcgtcggtat attgcagttt gccgtgacct
    27841 tccatttgcc acatggcggg gacggtaata tgcccccagt cagccattaa ctcagaggtg
    27901 aaggcttctg gtacttgcag cggatggtca aaaaagtgga aattccactg accgcttaag
    27961 ggcagaaaca ggctgctggt ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat
    28021 gaaaaaaagt acgcacgcgg cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag
    28081 cgattcatag ggcctcccgg tagagagaaa tcggaaccat tcatggtgat ggtagtttgt
    28141 gtaattgctg gggagtttag taaaattttt actaaaaagt agcaggtaaa tgcatttact
    28201 ttaactggat cacgaaaata aacggcagca ctgtccggca aaaacgattg ccggacagtg
    28261 tgtaagggga tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactaac
    28321 agcggcagcg cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc
    28381 atcatctcgg aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg
    28441 atatcgttaa cgctgataag cgaaatatcc tgtgggatgt tcaggcctcg ttcatgaatt
    28501 gcccgcagta cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct
    28561 tcccgcgcca gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc
    28621 cagatatcct cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca
    28681 cgaatatccg ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc
    28741 tggttgatat agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca
    28801 tcgtaaccgc tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg
    28861 gcagcggcgc gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta
    28921 atgtctggta agccgctgtg ttcataacag ttggtgagct cgatgcccag cttttcgcac
    28981 tgggtttcaa tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc
    29041 tggtagctgt agatagccag aatatggtgt tggttgactg cacctgtctg gagtttacgg
    29101 gcactactgg tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct
    29161 tctttcacat tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg
    29221 ccagcttcga ttgcgatgtc ttttagtgtt gccatgttta ccttttttac tttccctgat
    29281 gtgcgccccc attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc
    29341 gtttctttga cgctgtaacg gtaacacagc ggctcacgaa tgtgatcacg cctgcattct
    29401 ccatttttag cctacaggat tgagaaaaac ttttgctata gtttagtgaa attttacccg
    29461 aagggaggaa acaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt
    29521 cggaaagatt gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt
    29581 tgatgtgggg gaaaaaacac tgcaaaccgt gaccagcctg gtgccgtact acagcgaaga
    29641 agagctgatg gggaaaacgg tggtggtctt atgcaatctg caaaaagcga aaatgcgtgg
    29701 tgaaacgtcg gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgtt
    29761 gttaacgccg gagcggatga tgcctgcggg cgtacgcgtc gtataaaaag atcggatggc
    29821 gacgtcgtat cgccatccga tttgatatta cgcttcttcg acacttactc gcatggccgc
    29881 cagcgcctta cgcgccgctt tgatcaccag ttcacactgt tcaatggtca gtgtcagtgg
    29941 cggttcaatg cggatcgttt tggcgttatt gagcgttccg gccaccagta cgcgctggcg
    30001 gaacatctcg ctggcaaagt tatagccgat ttcgttatca acaaactcaa ttgccatcaa
    30061 catcccttta ccacgcgctt cctgtaccag atcgggatat tcccgcgcca gttgacggaa
    30121 accgtccagc aacatatcgc ctttttgctc agcctgagcc ggtaagttct gctccagcaa
    30181 cacattgatg gtcgccagcg ccgccgcaca ggccagcggg ttgccgccaa aggtggtggt
    30241 atgcaggaat gggttgtcga acagaactga aaacacctct tcagtggcga tggtcgcgcc
    30301 aatcggcatc acgccgccgc cgagcgcttt ggcaaggcag aggatatccg gctgtacgtt
    30361 ctcatgctcg caggcgaaca tcttgcccgt gcgccccatg cccgtttgta cttcatcgag
    30421 gatcatcagt gcgccgaact catcgcatag cttacgtacg gcggtgagat agcccggcgg
    30481 cggcagaatt acgccacctt caccctgaat cggttcgagg atcaccgcag ccacatcatc
    30541 accggttttt ttgcactcgt taagagccgt gcgcatggct tcgatattgc caaacggcac
    30601 atgacggaag cccggcagca gtggcataaa cggtttgcgg aaggtcgatt tcgccgtggc
    30661 cgacagcgcg ccaagtgatt taccgtggaa cgcgccgctg gtggcaataa aagtaaactt
    30721 gccgcgcggt gactggtaag ctttcgccag cttcagcgct gcttcaacgg actcggtgcc
    30781 gctattacag aagaagctgt atttcagttt accgggcgtt agcgcagcaa gggttttcgc
    30841 caacatcgcc cgtaacggat cgagcagctc ctggctgtgc agcggttgtt tcgcaagttg
    30901 attctgtacg gcggaaacca caactggatt acggtgcccc acgttgaaaa ttccaaaacc
    30961 tcccaggcag tcgataaact cctgtccctg ggtgtcgaca agcgtattta aacctcccgc
    31021 ttgccactct acggctccgt aatccccgcc ggcggtaaca gatttgcgat actctaaaaa
    31081 ccccggattg acatgctctt tgaagtattc aatcacctct cggttaagtg ctttcatctc
    31141 ctcatgatcc agcgttcgct tctcaatgag attcagggcg tgggcgctgc acgctaaagc
    31201 cgatgcgctc gaaggtaacc tgttcaaaat atgctccgga ggctcgcgta tcacatgata
    31261 ccgatttaag tattgcaggg attgcgccac tccggctgcg atcgcgaaaa tcgggataaa
    31321 cacaaattcg gttatcattt gtgcaatatt taatgctatc tgttaacatt tgttgttatt
    31381 ttgcacggtt tttgtcgggt atggatttga tgattgctgc gcaaatgcac gttttcaggg
    31441 caataattgc ctgatttttg ggcaagataa ttaacgtttt cgtatagtaa cagagtgaaa
    31501 gtattttaaa tcaaagaatt agatttgtgc tttaatcgca aattgcgatc taaatcaaat
    31561 taatcggtta aagataaccg cagcggggcc gacataaact ctgacaagaa gttaacaacc
    31621 atataacctg cacaggacgc gaacatgtct tctcatccgt atgtcaccca gcaaaatacc
    31681 ccgctggcgg acgataccac tctgatgtcc actaccgatc tgcaaagcta tatcactcat
    31741 gctaatgaca cttttgtgca ggtgagcggc tttaccttgc aagagttaca agggcagccg
    31801 cacaacatgg tgcgtcaccc ggatatgcca aaagcggcgt ttgcggatat gtggttcacc
    31861 ctgaaaaaag gggagccctg gagcggcatc gtgaaaaatc gccgcaaaaa tggcgaccat
    31921 tattgggtgc gggccaatgc ggtaccgatg gtgcgcgagg gaaaaatcag tggctatatg
    31981 tcgattcgta cccgggcgac ggatgaagag attgcggcgg tggagccgct gtacaaagcg
    32041 ctgaacgccg gacgtaccag taagcgtatt cataaaggcc tggtggtgcg taaaggctgg
    32101 ctgggtaaac tgccttcatt accgcttcgc tggcgggcgc gtggagtgat gaccctgatg
    32161 tttatcttgc tggcggccat gctttggttt gttgctgccc cggtggtgac gtatttcctc
    32221 tgtgtgttag tggtattgtt ggcaagcgct tgttttgaat ggcagattgt gcgcccgata
    32281 gaaaatgtcg cccgtcaggc gctgaaggtg gcgaccggag agcgtaatag tgttgagcac
    32341 ctgaatcgca gcgatgagct ggggctgaca ttacgcgcgg tagggcagct tggcctgatg
    32401 tgccgttggc taattaacga tgtctcaagc caggtgtcca gtgtcagaaa tggcagtgag
    32461 acgctggcga aaggcactga tgaactgaac gaacataccc agcagacagt tgataacgtt
    32521 cagcaaacgg tggcgaccat gaaccaaatg gcggcgtcgg tgaaacagaa ctctgccacg
    32581 gcgtcggctg ccgataaact ttctatcacc gccagtaatg cggcagtgca gggtggggag
    32641 gcgatgacca cggtgatcaa gacaatggac gatatcgccg acagtaccca gcgcattggc
    32701 accattactt cgctgattaa cgatattgcg tttcagacca atattctggc cctgaatgcg
    32761 gcggtggaag cggcgcgtgc cggcgaacag ggcaaaggtt ttgcagtggt ggcaggggaa
    32821 gtgcgtcatt tagccagccg cagcgctaat gctgccaacg atattcgcaa gctgattgat
    32881 gccagtgctg ataaggtgca atccggttcg cagcaggtac acgccgccgg acggacgatg
    32941 gaagatattg tggcacaggt gaaaaacgtc acccagttga tcgcccagat tagccattca
    33001 acgctggaac aggccgatgg gctttccagc ctgacccgtg cagtggatga gcttaacctg
    33061 atcacccaga aaaatgccga gctggtggaa gagagtgcgc aggtgtcggc gatggtgaaa
    33121 caccgcgcca gccgactgga agacgcggtg acggtgctgc attaatcggt atgccggatc
    33181 cggcggttgg agcacaatgc ctgatgcgat gctggcgcat cttatcaggc ctgcggggtg
    33241 tgtagcgggt gtaggccgga taaggcgcgt caagcgtcgc atccggcaat tgcaccgcgc
    33301 cactggcgga tgcggcgatt aatccagttg cgtaatatca aaagcggcgc ggtcgatcac
    33361 cgcaatgatc ttttttattt gtgcatcact gatatcgctc tggttgacgt gtaaatccag
    33421 caccgcttta aaattatcca gcgcccgctt catttgcggg ttctggcgca gcgccgcgcc
    33481 aacgcaacgc gctttgatgc gttcttcaat catctccacc tgttcgcggt tttcttccag
    33541 ccactgcgcg ccttgttcgg tcagcgcaat ctgcttctta ccaccttcct cttcgcggat
    33601 ggtaatcagc gactgctcct gcagaaaatc cagcgttggg tagatgacgc ccgggcttgg
    33661 ggtgtaattc ccctgggtta ggttctcaat cgctttaatc aattcgtaac cgtggctgtc
    33721 atcgcgcgag agaatatcca gaatcaccag acgcaattca ccgtgaccaa agaaacgttg
    33781 ccgacgaccg ccgccgcgac cgtggcgacc accgcagcat tgaccgtgtt catgctggtg
    33841 accgtgtccg cagtgctcgt gttctgactt ctcacctttg cagcagccct catggcgtgg
    33901 ctggccttca tgtttacaac acccttcgtg atgatggctc atatcagcct ccttttgctt
    33961 gattagatat atctaattta tatctgatgt gagcattact gcaagtacaa aaatgatttt
    34021 taaacgtgga agcacctcac atccaggccg gataagacgt tcacgccgca tccggcagca
    34081 ttgctatggc actgaatgcc ttcacatact tcagatatat cagttctaca aatcgcttgc
    34141 atttatcatg attaacaatc attatcattt acgaaaatca tttagatata tctgattact
    34201 atcacgaagg cgataacaat gaataactcc ccccgctacc cgcagcgcgt tcgcaatgat
    34261 ctgcgcttcc gtgaactgac tgtgttacgc gttgagcgca tcagcgccgg ttttcagcgc
    34321 attgtcctcg gcggcgaggc gctggacggt tttacatcgc gcggctttga cgatcacagc
    34381 aaactcttct ttcctcaacc tgacgctcac tttgtgccgc caacggtaac ggaagagggc
    34441 atcgtctggc cggaaggacc acgcccactg tcgcgtgact atacgccgct gtatgacgaa
    34501 ctacgccatg aactggcgat cgatttcttt attcacgacg gtggggtcgc cagcggctgg
    34561 gcgatgcagg cgcaaccggg cgataaactt acggtggcag gtccgcgcgg ttcgctggtg
    34621 gtgccggaag attacgcgta tcagctgtat gtctgcgatg aatccggaat gcccgcactg
    34681 cgccgccgcc tggaaacgtt gagcaaactt gccgttaaac cgcaagttag tgcgctggtt
    34741 agcgtgcggg ataacgcctg tcaggattat ctcgcgcacc ttgatggttt taatatcgaa
    34801 tggctggcac atgatgagca ggcggtagat gcgcgtctgg cgcagatgca aatccctgcc
    34861 gatgattact tcatctggat aaccggcgaa ggtaaagtcg ttaagaattt aagccgccgc
    34921 tttgaagcgg aacagtatga cccacagcgg gtacgtgcag cggcttactg gcacgcaaag
    34981 taactgatat ataaaaagaa aaaggctgac gatttctcgt cagcctttgc tatatctggt
    35041 ggcccctgct ggacttgaac cagcgaccaa gcgattatga gtcgcctgct ctaaccactg
    35101 agctaagggg ccatggtagc ggattataaa gtaactccgt gtcgcaatcc agccattacc
    35161 gtgcgcctgc tgtttttata aacaatgtat tttcaatccg ttatactttt cctggtgatg
    35221 ttagaaagga gtaaatatgg ttgaggatat tttggctcta gggttacggg tcgtgttttg
    35281 cggtatcaac cctgggcttt catccgccgg gactggtttt ccctttgctc atccagcaaa
    35341 tcgcttctgg aaggtgatat atcaggccgg gtttaccgac cgtcagttga agccgcagga
    35401 ggcacagcat ctgctggatt atcgttgtgg cgtcaccaaa ctggtagacc gtccaacggt
    35461 gcaagccaat gaagtttcaa agcaggagct gcacgcaggt gggcgtaagc tgattgaaaa
    35521 aattgaagat tatcagccgc aggcgttggc gattctgggc aaacaagcat atgaacaggg
    35581 attcagccag cgcggtgcac agtgggggaa acaaacgctc accattggtt cgacgcagat
    35641 ttgggtgctg ccaaatccca gcggtttaag tcgcgtttca ctagagaaac tggttgaagc
    35701 atatcgcgag ctggaccagg cgctggtagt gcgtgggcga taaaaaacgc caccgaaaag
    35761 gtggcgtttg tgcaaaggga ctagtgccgg gtgcggcgta acgcctgatc cggcctaccg
    35821 attaatcgtc caggaagcta cgcaacactt cagaacggct cgggtgacgc agtttgcgca
    35881 gcgccttcgc ttcgatctga cggatacgtt cgcgggtaac gtcgaactgt ttacccactt
    35941 cttccagcgt gtggtcggtg ttcatatcga taccgaaacg catacgcaga actttcgctt
    36001 cacgcgcggt caggccagcc agcacgtcgt gcgttgccgc acgcaggctt tcggtggtcg
    36061 cagaatccag cggcagctcg agggtggtat cctcgatgaa atcccccaga tgcgaatctt
    36121 catcatcacc gatcggcgtt tccatggaga ttggctcttt ggcgatcttc agcactttgc
    36181 ggatcttgtc ttccggcatc agcatacgtt cagccagttc ttccggcgtc ggttcacggc
    36241 ccatctcttg cagcatctgg cgagaaatac ggttgagttt gttgatagtc tcaatcatat
    36301 gcaccggaat acggatggtg cgcgcctgat ccgcgataga acgggtgatc gcctgacgga
    36361 tccaccaggt tgcgtaggtg gagaacttgt aaccacggcg gtattcgaat ttatcaaccg
    36421 ctttcatcag accgatgttg ccttcctgaa tcaggtcaag gaactgcaag ccacggttgg
    36481 tgtatttctt ggcgatagaa ataaccagac gtaagttcgc ttcaaccatc tctttcttcg
    36541 cacggcgggc tttcgcttca ccgatggaca tacgacggtt gatatcttta acctgctcga
    36601 tggtcaggcc ggtttcttct tcaatctgct gcagtttctg cagggcgcga tgcacttctt
    36661 cagagacatc gtgcagtttt tccgaccacg gcttgttcat cgcaattgcc gcgttgaacc
    36721 aggtatcgct ggtttcgttg ccggtaaaca gggtaatgaa gtttttcttc ggcattttgc
    36781 actgctcaac gcagagcttc atgatcagac gttcttgcgt acgaacgcgg tccatcatga
    36841 cgcgcatgct gttgaccagg tagtcaaact gcttcggcac caggcggaac tgtttgaata
    36901 cttcagacag tttcaggatc tcttcctgag cggcagcgtg actgcgacct ttcgctttga
    36961 tggtgtcacg cgttacaacg tactgagcgc gtagttccgc aaatttttcg cgagccagtt
    37021 ccgggtcgat gctgttgtca tcatcggcgc tgtcgtcatc gccatcttct tcgtcttcat
    37081 cttcgtcatc gtccagatct tcctgggaaa gctcagaacc gacgtgagtg gcggtaggtg
    37141 ccagatcttc ttctgcgttc gggtcaacaa agccggtgat cagatcggac agacgcgctt
    37201 cttctgcttc aacacgatcg tactgttcca gcagataggt gatcgcttcc ggatattcag
    37261 caacggagca ttgaacctgg ttgatcccgt cttcaatacg cttagcgatg tcaatttcgc
    37321 cttcgcgggt caacagttca acggtgccca tttcacgcat gtacatgcgt accgggtcag
    37381 tcgtgcgccc gatttcagat tccacgctgg aaagcacctg cgcggcggct tcggcagcat
    37441 cttcgtccgc ggtgttttca gccagcatca gatcatcggc atccggtgct tcttccatca
    37501 cctgaatgcc catgtcgttg atcatttgga tgatgtcttc gatctgatct gaatcgacga
    37561 tatcttccgg cagatggtca ttgacctcgg cataggtcag atagccttgc tccttaccac
    37621 gggtgacaag aagtttcagc tgtgactgcg ggttttgctc cataagacgg tatccacact
    37681 tatttcatga ggttggtgtt gggcgattga cccgatgtcc ttcagccgtt aacgctgaaa
    37741 tccaacggtt gtttaccgcc aacgataatt acgagggcgt acttatatat ttgccgctgc
    37801 ctctcagtgc ggctgtcggg ggcttcccga tcgctcttcg gcacttaagc cgttaaatca
    37861 ctttttcgcc agctccaggt ttaatgtcca gagctccagg cgttcttcgt tgcttaaacc
    37921 atgcgtgcgc tcacgagcga ttaactcttc ctggcgcagt tcaagcagcg aatcaaacat
    37981 atggttgagt gagtcggtga aggtttgctc agcaatattc ttatctgcta tatcgtccca
    38041 catcgacagt ttttcaaggg tggcagcatt atttgtacca cgatagtgct ctaaaagttg
    38101 cccggtggtc agacctggct gggagagaca agtgttgacc agttctctga ataagccaag
    38161 tccagggagc ttattttcat ccagattctc aagcggcggg accaacgtcg ctaattctgg
    38221 attttgcacc agcaacccta taagtatacg catggtcgtg cgttttagct gcggaacagg
    38281 gcgagaaacg ccgctctctg ccgcttttgg cattaatcgt tcaagctggc tgtcatcaag
    38341 tatgcctaat ttgttgccta attcctgacg aagatatatt cgcagcgttt cgcccggcac
    38401 ttgcgatatc aatggcagtg ccagcgtact caaacgtgcg cgcccgtcag gggtactcag
    38461 atcaacttgc ggcatcaggc tgttaaacag aaatgcggag agtggcatcg cctgctccat
    38521 ccgcgcttca aacgcttctt taccttcttt tcgtaccagc gtgtcagggt cttcgccatc
    38581 cggcaaaaac ataaagcgta gctgacggcc gtctgtcatg taaggcagcg ccgtttccag
    38641 cgctcgccat gcggcatcgc ggcctgcacg gtcgccgtca taacagcaaa tgacattgtt
    38701 ggtcgcgcgg aacaacagtt gtatgtgatc ggcggtggtt gacgtaccta acgacgcaac
    38761 ggcgtaatta atgccgtatt gcgccagcgc caccacgtcc atatagcctt cgaccacaag
    38821 cagacgattg ggttcagcgt tatcctgctg cgcttcataa agaccgtaaa gctggcggcc
    38881 tttatggaaa atgtctgttt ccggcgagtt caggtatttg ggggtatcgt tgcccagcac
    38941 gcgcccgcca aaaccaatca cccgaccgcg tttatcgcga atggggaaca tcacccgctc
    39001 gcggaaacga tcgtaactgc gtccctgatc gttagtgacc aacatccccg catcaatcaa
    39061 tgactggcga ttttctggat tgccgccaaa ccgcttcagg acgttgtccc agccgggggg
    39121 cgcaaaacca atcgcaaagc gagcgataac ctcgtggctt aatccgcgtt tttccagata
    39181 ctggcgcgca gacgtggcaa caggttgttg taaagattgt tggtaaaacg tattcagacc
    39241 gtccatcaac tgataaagcg tttgcctctg atggcgctcg atctggctgg ggccgctgcc
    39301 tgcttcaaat ggcacttcaa gattgtgcat tgctgccagc tcttcgaccg tttcaacgaa
    39361 ctcgagcttg tcgtagttca tcaggaagtc gatcgcgttg ccgtgcgcgc cacatccaaa
    39421 gcagtggtaa aactgtttct caccgttaac ggtgaatgac ggggttttct cgttgtggaa
    39481 tggacaacac gcgtggaaat tcttgccctg ctttttcagc ttcacacggg catcgatcag
    39541 atcgacgatg tcagtgcgtg ccagcagatc attaatgaat acgcgtggga ttcgtccagc
    39601 cataggcccc gatttttagc aattcataaa cgaaaataag ccgcgcattc ctttcggaag
    39661 cacggcctta caactacaac tcggtctgat cggagagcaa cgctctcggg gaattagtac
    39721 agacgagtgc ggcgtgcgtt ttcgcgagcc agtttcttcg cgtgacgttt cactgcagaa
    39781 gctttagcgc gcttacgttc ggtagtcggt ttttcataga actcacgacg acgaacttcc
    39841 gccagaacac ctgctttttc gcaggaacgc ttgaagcgac gcagagctac gtcgaacggc
    39901 tcgttttcac gtactttaat taccggcatg tgcctctcac ctttgattaa ttcggtttgc
    39961 cgctggcatc aacgccagct tatttcaaaa tggtgcggaa ttttactgca attgctgctg
    40021 ctttgtaaag caccgcggcc ttttttgaaa gggactttta taagggtgag gagtatacac
    40081 gaggctttct ccaggggcga acaaagtttt acatcaaccc gcattggtcc tacactgcgc
    40141 ggtaataaag cgaggtaaaa caagtcatgc gtgtactggg tattgaaact tcctgcgatg
    40201 aaaccggcat cgccatttac gacgatgaaa aaggtttgtt agccaaccaa ttgtatagtc
    40261 aggtgaaatt gcacgctgac tacggcggcg tcgtgcctga actggcctcc cgcgatcacg
    40321 tgcgcaaaac cgtaccgttg atccaggcgg cgctaaagga gtctggttta acggcaaaag
    40381 acattgatgc tgtggcctat accgcaggcc ctggattagt cggcgcactg ctggttggcg
    40441 caaccgtggg gcgttctctg gcgtttgcct ggaacgttcc ggcaatcccg gtacaccata
    40501 tggaagggca tctgttagcg ccgatgctgg aagataaccc gccggaattt ccgtttgtcg
    40561 cgctgctggt gtccggcggt catacgcagt taatcagcgt gactggcatt ggtcagtacg
    40621 agctgctcgg cgagtctatc gatgatgccg ccggtgaagc gtttgataaa accgcgaagc
    40681 tgctggggct ggattatcct ggcggaccgt tactgtcgaa aatggcggct cagggtactg
    40741 ccgggcgctt tgttttcccg cgtccgatga ccgaccgtcc ggggctggat ttcagctttt
    40801 ctggtctgaa aacctttgcg gcgaacacga ttcgtgacaa cggcaccgac gaccagacgc
    40861 gtgctgatat cgcccgcgcc tttgaagatg cggtggtcga tacgttgatg attaagtgta
    40921 agcgagcgtt ggatcagacg ggctttaagc gactggtcat ggcgggcggc gtgagtgcta
    40981 accgcacgtt acgggcgaag ctggcggaaa tgatgaaaaa acgccgcggc gaagtgttct
    41041 acgcgcgtcc ggaattttgt actgataacg gcgcgatgat cgcctatgcc ggaatggtgc
    41101 ggtttaaagc aggcgcgacg gcggatctcg gcgttagcgt gcgtccgcgc tggccactgg
    41161 cagagttacc ggctgcgtaa aactatccgg tgccggagag caatttccgg caccgtcctc
    41221 acttaaagca acaccacggg catccacagc aagccggtaa tcaccagcaa cacgaggaat
    41281 atcagtccaa aaatcgcccc cagtcgccag taatccaccg ttggcagata accactaccg
    41341 tagtaaatcg gactgggtcc ggtggcgtat ggtgtaagaa tgctccccag accaattgcc
    41401 gcgccaacca tcaggcagaa caccggcagc gggatttccg gcatcgccag cgcagcggca
    41461 atcatcatcg gtgcgagggc ggaggtatac gccgtggcgc tggcgaaaaa gtagcgcagc
    41521 agataaaaca ccacaatcaa cgccaccatc accatcgttg gcgaataacc gcttaagctg
    41581 cctgctaaca gtttgccaaa ccagctaata aaaccagtgt tgttgagtcc ggtagcgagg
    41641 gtgataagcg aggccagcca gaagaaaacg ttccacgccg ctttattact gacaatgtcg
    41701 tcccaactga taatgcgcag aagcagcatc agtgccacca cgctgtaacc gaccatcgcg
    41761 gcatcgatat aatcaccgcc gaaaatccac agcaccagcg cgcctaccat cagccccagc
    41821 atccgttttt cacgcgaaca gagcgggccc attgcctgca gttccgtctc tgcccagcgc
    41881 ggcacctgat cgccagactt cagtaccggc gggtacagca cataagccag ccagggaacc
    41941 agcagcacca gcaaaatact gagcggcaac atcccgagga accagtcgcc ccaactcagc
    42001 gtggcgtgag atgcgctttt catcagtcca attaacagca agtttggtgc catcgccgtc
    42061 aagaaaatgg cgctggtcac gcagtcggca acaatcccca tccacatgat gtacgagcca
    42121 atgctgcgcg aactgctgtc gtttggttgt gattgataga gcggtggcag gttacggatg
    42181 atgggataga taatccccgc accacgcgcc gagttggacg gtgttacagg tgcgaggatc
    42241 agctcggaga acatcaccgc atagccgaga aacagcgtgc gatgtcccat ctttttcacc
    42301 agaaacagcg caatgcggcg tccaagcccg gttttttcat agcctgtgcc aaacataaag
    42361 gcggcgaaaa tcagccagat aaccgaatta gaaaaaccgg aaaccgccca cgagagggat
    42421 tttgcagtga atttaaagcc tggctgggcg agctgttcgg ggctgaacag cagccaggga
    42481 gagagtatgg cgatgatgga gatacccacc atcgccacca cggcacccgg cacgggttcg
    42541 aggatcagtc caacgatcac gccagtaaaa acggcaaagt agagccaggt atggttctcc
    42601 agccctgcgg gaaccggaag tagagcaata atggcgatga ccgccagcgg cgcaagatat
    42661 cgccaccatt cagtggaagg tttcatcgtg ttatgcctgc gaaaaaggag aggggtgagc
    42721 ctctccggga gggttatttg atgtaatgga cgtgctcgca gatctcttcc acgatgggat
    42781 cgcggcgttc ggcgaacagc tttttgtttt cggctatcag gttgttgccg tgggtatcaa
    42841 tagagacaat cagcgggccg aactctttga cccggcagac ccacagtgac tccggcatcc
    42901 cgagttctgt ccagtgcact tcttcaatct cttctacctg ggttgccgcc agcaccgcgc
    42961 agcctgccgg gaaaatcaca tgtagcgcct tgaatttctg gcagccttct tcggtcagcg
    43021 gccccatacc acctttgcca accaccagtt tcacgccggt ctgctcgata aattcacgtt
    43081 caaaactttc catacgcata ctggttgtcg ggccaacgga aaccatctcc catttatcgc
    43141 cgtttttgcg cacgatgggg ccagcgtgga aaatcgcttt gccgttgaga tcgtaaggga
    43201 tcggacgttt cagttcgatc aagcggcggt gacaaacgtc acggcaggtc accagcgtac
    43261 cggtcaggta gatcacatcg ccgacgcgaa tatcttgcag atcttcagct ttgatcgggg
    43321 ttgtcaggat ctttttcata acgcgctccg ggtgtgagac agattttcaa aggtgagatc
    43381 ggcatgaacc agcagtgtgc cgcgacgatg cgcccagcag cctgtagaaa cagcaacgcc
    43441 gatggttgac ggatggcggg cggcagattc gatatgcacg cccatcactg aactgttgcc
    43501 agtcagccct tgtgggccaa ttcccagacg gttgagtcct tcttccaggc gtagctccag
    43561 ctctgccgct tttggattgg gatggcggct gccaatcggg cgcaaaatgg cttttcgcga
    43621 gagcacggcg gcggtttcca ccgaggtggc gatgcccacg cccaccagta ccggtggaca
    43681 ggcgtttacg gcgagggtgg agatattttc gaagacgaat ttcaccacgc cttcgtagcc
    43741 ttctgacggc attaacactt tcgagcggcc aggtagcgtg cagccgccgc ctgccatgta
    43801 aacttcgatt tccgcatcgt cattgtcggg gatgatgtcc caggtgaccc acggtacgcc
    43861 gctaccggta tttttgccgg tgtttacttc gtcaaaaatt tctaccgcat tgtgacgtag
    43921 tggcgctttg acggttgcct cttccacggc ttgtttgagt atgctttgca gctcgccaag
    43981 cagtgggaag cgggaaccga ctttaacgaa gaacataatc tccccggtgt cctgacaggc
    44041 aggacgattc aggtcaatcg ctttttgcat gttgtcgaac atcgtatggt agataatttt
    44101 ccccatcgac gacgtttcgg catcctttag ctgttttagt ttatccacca cgtcatcagg
    44161 cattcgggta gaaatcatgg cggtaaagtt agcgacaatc tctgtcaact tattcactgc
    44221 ctgttgctta ttactttcgc tcatcatggc aactccagtt tttgtatttg tctggaaaaa
    44281 ttcaccgcca caaactacca ctaaaaatgt ggttttatat tttttgccac cgtgaatatg
    44341 taattaacag ttcgtgaatc aacgcgaaaa taagaatgaa tattgcgcca gagcagggaa
    44401 tggcaatgtt ttgcgaaata cactgcgctt atcacaactg cggataatta acgatgctga
    44461 atagctggcc tttagccaaa gacttgcagg tactggtaga aatcgtccac agcgggagtt
    44521 ttagtgctgc agcggcgacg cttgggcaga cgcctgcatt tgtcaccaag cggatccaaa
    44581 tccttgaaaa tacgctggca acaacgctgc tgaaccgctc cgctcgcggc gtggcgctga
    44641 ctgaaagtgg ccaacgttgt tacgaacatg cgctggaaat cctcacccaa tatcagcgac
    44701 tggtcgatga cgtcacgcaa atcaaaacgc gcccggaggg gatgattcgt attggttgca
    44761 gttttggttt tgggcgcagc catattgcgc cagccattac cgaacttatg cgcaattatc
    44821 ctgagttaca ggtgcatttt gaattgttcg atcggcaaat tgatttagtg caggataata
    44881 ttgatctgga tattcgtatt aatgacgaaa ttcctgatta ttatattgcg catctgttaa
    44941 cgaaaaataa aagaatatta tgcgcagcac ctgagtatct gcaaaaatat ccgcagccac
    45001 aatcattaca ggaattaagt cgtcatgact gtctggtgac caaagagcgc gatatgaccc
    45061 acggaatatg ggagttgggg aacggtcagg agaaaaaatc ggtgaaggtt agcgggcatc
    45121 tttcctccaa tagcggcgag atagtgttgc aatgggcgct ggagggcaaa gggattatgc
    45181 tgcgttccga gtgggatgtg ttgccgtttc tggagagtgg caaactggtg caagtattgc
    45241 cggagtatgc acaaagcgcc aatatctggg ctgtttaccg ggagccgctc tatcgcagca
    45301 tgaaattacg tgtctgcgtg gaatttctgg cggcatggtg ccagcaacgg ctgggcaagc
    45361 ccgatgaagg ctatcaggtc atgtagatcc accagaaatc actcgggatc cttttcgcgc
    45421 tttcttttga atttcgtcca gatttttgtc tcctgacgac gccacagacg ctggatgttg
    45481 tcatgatgac gcagcaggat caggcaagag agcatcgaaa ccgggaaggt gaattgtggc
    45541 ttaaaccacc atacataaaa cggagcaatc agtgcactga caatcgctcc cagcgacgag
    45601 tatccgctca atagcacggt cagtaaccag gttcccgcca ttactccggt gagatcccag
    45661 ccaatgggtg cgatggcacc aaaagcggta gcaacgcctt ttcctccttt aaatccgaag
    45721 aaaacgggcc agatgtgtcc aagacaggcg gcgatggcaa ttaagcctag ccagaagggg
    45781 ctgacaccta attcatacgc gccccagacg ggcaacattc ctttcagaac gtcgaaaatc
    45841 agtactgcta cggctgctcc cttgccaccg atacgtaaca cattggttgc gcctggattg
    45901 ccggagccgc tggttcgcgg atcgggcagc ccacacaagc ggcaaaccag aatggcactg
    45961 gaaatggagc cgcagaggta cgcgatgagg atcattccag gcgcgattgc actcataagc
    46021 tgttccgttt tgaaaattcg tgttcaacga tgaatctgtg gataatacgc acatttcgcc
    46081 ggaagtggta tccggttagc caaaaagcag gcaggacgtg atggatattg tatttataga
    46141 gcaactttcg gtaatcacca ctattggtgt ttacgactgg gaacagacca tcgaacagaa
    46201 gttagtgttc gatatcgaaa tggcatggga taaccgtaaa gcggcgaaaa gtgatgatgt
    46261 ggcggattgc ctcagttacg ctgacattgc agaaacggtg gtcagccacg tcgagggggc
    46321 gcgttttgcg ctggtggaac gcgtggctga agaggtggcg gagctgctgt tagcacgctt
    46381 taactcgccg tgggtgcgta tcaaactcag caagccaggc gcagtggcgc gggcggcgaa
    46441 tgttggcgta atcattgagc gtggcaataa tctgaaagaa aataattaat tttacagctg
    46501 ttaaaccaaa cggttataac ctggtcatac gcagtagttc ggacaagcgg tacattttaa
    46561 taatttaggg gtttattgat gagcgatatg cactcgctgc tgatagcggc aatattgggt
    46621 gtggtcgaag gattgacaga atttctgccg gtatccagca cgggccatat gattattgtc
    46681 ggtcacttgt tggggttcga gggcgacacg gcgaaaacct ttgaagttgt gatccagtta
    46741 ggatcaattc tggcggtagt agtgatgttc tggcggcgtc tgtttggcct gattggcatc
    46801 cactttggcc gcccgttgca gcacgaagga gaaagcaaag gtcgtttaac gctgatccac
    46861 attttgctgg ggatgattcc ggcggtagta ctggggctgt tgttccacga cacgattaag
    46921 tcattgttta acccgataaa tgtgatgtat gcactggtcg ttggcggttt gttgctgatt
    46981 gccgccgaat gcctgaagcc gaaagagccg cgtgcgccgg gtcttgatga tatgacctat
    47041 cgtcaggcgt ttatgattgg ctgtttccag tgtctggcgc tgtggccggg tttctcccgt
    47101 tccggggcca ccatttcagg tgggatgctg atgggcgtga gccgttacgc tgcttccgag
    47161 ttctcgttcc tgctggcggt accgatgatg atgggcgcaa cggcgctcga tctctacaaa
    47221 agctggggct tcctgacaac cggcgatatc ccgatgtttg ccgttgggtt tatcaccgct
    47281 tttgtggtgg cgctgatagc gattaaaacc ttcctgcaat tgattaagcg catttcgttt
    47341 atcccgttcg ccatttatcg ctttattgtg gcggctgcgg tgtacgtcgt gttcttttga
    47401 ttgagaaaag gttcgggagc aggcctggtt ttctccctcg ctctgtaggg agagggccgg
    47461 ggtgggggtg attgttcgca ccggtgctgg tctgttcccc tcaccctaac cctctccccg
    47521 aaggggcgag gggatggttc gtgttaacgt ttagttttat actcagtcag gctttgggca
    47581 acgttgttcc ttccaactgg ctaccgccgc aatccgccgt cgggtcagct cctcgcgaat
    47641 ctccacacct ttaaatcccg cttcaacgac cgcttttgtc ggcactgact gcgccacttc
    47701 ccaggcttcg cgcaaccaac ggccttgcgg gtagtccgct gattcaaaac cggttctgcc
    47761 gcgtacgtca gcctcgctgg tcagcgccag ttgctcgaca cgctgcggtt tacgccaggc
    47821 atcgatggaa tcaaataatt tgacgatggt ttttgggttc agcattggga aggtgtggat
    47881 gagatcgtga aactcagcca ccagtctggc taaatcgcga atttcatttg gcacgcgcag
    47941 acgctggcat agttgttcca ctaacttaac acccgccggg ccatgcccat gatgacgcgg
    48001 ccaaagttct ggcggcgtca gtcctttacc gagatcgtgg cataaagttg cgaaacggac
    48061 atcaacctgc ggactcagca tcgccgccat tgagagcgtc attaaggtat gaatacccgt
    48121 gtcgatttcc ggatgccacc tggcaggggc cggaacgcca aacagggcgt caatttccgg
    48181 gaataaaacg cgaagtgcac cgcaatcgcg cagtacctgg aagaacacct ggggattgcg
    48241 ggtggtgagg gcgctttccg tctctttcca tacccgttca ggcgtcaggt gttccagttc
    48301 acccgcatgg gtcatctcgc gcatcagcgt cagagtttca tcggcaatac gaaaaccgag
    48361 gtgggcataa cgcgcagcaa aacgcgccac gcgcagaacg cgtaacggat cttcgccaaa
    48421 agcgggggaa acatggcgca acagacgatt ttgcagatcg cccagaccgt tgtacgggtc
    48481 gataatctcg ccgttatcgt cctgagccag cgcattaatg gtcagatcgc gacgcttaag
    48541 atcatcttcc agcgtgacat ccggtgcggc atagcaagta aaaccggtgt aaccggaacc
    48601 ggatttccgt tcggtacgtg ccagcgcata ctcttcatgc gttagcggat gcagaaacac
    48661 aggaaaatcg cggcctacct gctggtagcc cgcgtcgagc atctcctgtg gcgtactgcc
    48721 gaccaccacc caatctctgt ctttgaccgg tagccctaac aatgcatccc gaacagcacc
    48781 accgaccaga taaatcttca cgccatcatc ccttcgtcaa atacacaatt cctaataata
    48841 agacagtgca acgaagaagg cgatttagtt catccagcga tctttgcgtt tgcgcgttgg
    48901 gatcaggtgt ggcagtacca gaccgagcag caagccaagc cccagcacgc caccgccata
    48961 cataaaccat tgcatgatga tggtgcgctg tttgtcatcc aactgtacgc tggcggcatc
    49021 gacctttttc tgcgcgacaa tcagctcgtt tttcaatttc tgattttctt cttttaatcc
    49081 gttgatcacg ctgtcgctct gcgccacttt ttgctgcatt tctgcggtgc gctgattcca
    49141 ggtgttatcg atattggtga gtttatcggt cagggttttg acctgatttt ccagatctgg
    49201 cacacgggag cgcaggcttg gctcagtgct aagttgtttc aacgggatcc aggcggtacg
    49261 gccagagctg tctttcacct gggcataatt ggtgttggcg tcagtttgta ataaggtcac
    49321 ttcctcgccg gcgttaaccg tgcccacgag gcgataatga tctcccggac cgctacggac
    49381 ccaggtattc agttcatcag aaacatagcg cgtttcttca gcgtgtgaga cggcagtggc
    49441 gctaagtgcg agtaaagtta atccgatcag gcgtaatttt ggcatcaggc tgtcgttatt
    49501 gtcatggaaa gtggaacgat agtagtggca tcagtgtcgc tacgcaaagc attcgacatc
    49561 aatcggaatc ctctgtgtca ggtcttgcca ctgcttcaaa cttttacgcc cgtcaaattg
    49621 cccgttgcat ggcaatttgg cgcaaaatac tatctactga caaaaaagat cgcattaagt
    49681 tcgccgttca tcgtcactta tgtgacataa ccgataagta aaggccatgg ctcaggaaat
    49741 cgaattaaag tttattgtta atcacagtgc cgttgaggcg ttgcgtgacc atctcaatac
    49801 gctgggcggc gagcaccatg accccgtgca gttgctgaat atttactacg aaacgccgga
    49861 taactggctg cgtgggcacg atatgggctt acgtattcgt ggcgaaaacg gtcgctatga
    49921 gatgaccatg aaagttgcag gaagagtgac aggcggctta caccagcgcc cggaatataa
    49981 cgtggcgttg agcgaaccga cgctcgacct ggcgcagtta ccgacggaag tctggccgaa
    50041 cggcgaattg cccgccgatc tcgcttcccg cgtacaaccg ctgttcagca ccgattttta
    50101 tcgcgaaaaa tggctggtgg cggtcgatga cagccgaatt gaaatcgctc tcgatcaggg
    50161 ggaggtgaaa gcgggggaat ttgctgaacc tatctgtgag ctggaactgg aactgcttag
    50221 cggcgacacg cgcgcggtgc tgaaactggc gaaccaactg gtatcgcaaa ccggattacg
    50281 ccagggcagc ctgagcaaag cggcgcgtgg ctatcatctg gcgcagggca atccggcgcg
    50341 tgaaatcaaa ccgaccacca ttttgcatgt tgcggcaaaa gccgatgtgg aacaggggct
    50401 ggaagcggcg ttcgagctgg cgttagcgca atggcagtat catgaagaac tgtgggtacg
    50461 cggcaacgat gcggcgaaag agcaggtgct ggcagccatt ggccttgttc gccatgcact
    50521 gatgctgttc ggtggcattg tgccgcgtaa agcgagcact cacttacgtg atttgctgac
    50581 tcaatgcgaa gcgaccatcg cttctgcggt ttctgccgtg acggcggtct actctaccga
    50641 aacggcaatg gcaaagctgg cgttgaccga atggttggta agcaaagcct ggcagccgtt
    50701 tttagatgcc aaagcgcagg gtaaaatcag cgattccttc aaacgctttg ccgatatcca
    50761 tctttcccgc catgccgctg aactgaaaag cgttttctgc cagccgttag gcgatcgcta
    50821 ccatgatcag ttgccacgcc tgacgcgtga tattgactca atactgctgc tggcaggtta
    50881 ctatgatcct gtcgtcgcgc aagcctggct ggagaactgg caggggctgc gtcacgctat
    50941 tgcgaccggg caacgcattg aaattgaaca tttccgtaat gaggcaaaca atcaggaacc
    51001 gttctggttg cacagcggaa aacgttaatc aggcaaggat aaaatcgctt atgaagccgc
    51061 tctcttcacc gttacagcag tactggcaga ccgttgttga gcggctgcca gagcctttag
    51121 ccgaggaatc acttagcgca caggcgaagt cagtacttac ttttagtgat tttgtgcagg
    51181 acagcgtgat tgtgcatcca gagtggctga cggaactgga aagccaaccg ccgcaggccg
    51241 acgaatggca gcattacgcg gcatggttgc aggaggcgct cagtaatgtg agtgacgaag
    51301 ccgggttaat gcgcgagctg cggctattcc ggcggcgcat tatggtgcgc atcgcctggg
    51361 cgcaaacgct ggctttggtt actgaagaga gcatattgca gcagctcagc catctggcgg
    51421 agacgctgat tgtcgccgcg cgtgactggc tgtatgacgc ttgctgccgc gagtggggaa
    51481 cgccgtgcaa tgcgcagggc gaagcgcaac cgctgctgat tttaggcatg ggtaagctgg
    51541 gcggtgggga actgaatttc tcctcagata tcgatctgat ttttgcctgg ccggaacatg
    51601 gttgtacgca gggcggacgc cgggaactgg ataacgcgca gttttttacc cgcatggggc
    51661 agcggctgat taaagtgttg gatcaaccaa cgcaggatgg cttcgtctat cgcgtggata
    51721 tgcggctgcg tccgtttggc gaaagtggcc cgctggtgct gagctttgcc gcgttggagg
    51781 attattacca ggagcagggg cgcgactggg agcgttacgc gatggtcaag gcgcggatta
    51841 tgggcgatag cgaaggcgtc tatgctaacg agttgcgtgc gatgctgcgc ccgtttgttt
    51901 tccgtcgtta catcgatttc agcgtgattc agtcgctgcg caacatgaaa gggatgattg
    51961 cccgtgaagt gcgtcgacgt ggtttgaccg acaatatcaa actcggcgca ggcggcattc
    52021 gcgaaattga atttatcgtt caggtgttcc agctcattcg cggtgggcgc gaaccgtcgc
    52081 tgcagtcgcg ctctttactg ccaacgctca gcgtcattgc cgcgctgcat ctgctttctg
    52141 aaaacgatgc tgaacaattg cgagtggcgt atctgttcct gcggcgtctg gaaaacctgc
    52201 tgcaaagcat taacgacgaa caaacccaga cgcttccttc tgatgagctt aaccgtgcgc
    52261 ggctggcgtg ggcgatggac tttgctgact ggccgcaact gaccggggcg ctgaccgcac
    52321 atatgaccaa tgtgcgccgg gtgtttaatg aattgattgg cgacgacgaa agtgaaactc
    52381 aggaagagtc gctgtcggaa cagtggcgtg agctgtggca ggatgcgttg caggaagatg
    52441 acactacgcc agtgctggcg catcttagcg aggatgatcg caaacaggtg ctaacgctga
    52501 ttgccgattt ccgcaaagag ctggataagc gcaccatcgg gccgcgagga cgtcaggtgc
    52561 tcgaccatct gatgccgcat ctgctaagtg atgtctgtgc gcgtgaagac gctgccgtta
    52621 cgctgtcgcg cattaccgcc ttgctggtgg ggattgttac ccgcaccacc tatttagaat
    52681 tgctcagtga attccccgcg gcgcttaaac atttgatttc tctgtgtgcc gcgtcgccga
    52741 tgattgccag ccaactggcg cgttatccat tattgctgga tgaattgctc gatccaaaca
    52801 ccctttacca gccgacggcg accgatgcct accgcgatga gttgcgccag tatttgctgc
    52861 gcgtgccgga agatgacgaa gagcaacagc ttgaggcgct gcgtcagttc aaacaggcac
    52921 agctgttacg catcgccgca gcggatatcg ctggtacgct accggtgatg aaagtgagcg
    52981 atcacttaac ctggctggca gaggctatga tagatgccgt cgttcagcag gcgtgggttc
    53041 aaatggtggc ccgctacggt aagccaaatc acctcaacga tcgcgaaggc cgtggttttg
    53101 cggtggtcgg ctacggcaag ctgggcggct gggagttagg ctacagttcc gatcttgacc
    53161 tgatcttcct ccatgattgc ccaatggatg cgatgactga cggcgagcgg gaaatcgacg
    53221 ggcgacagtt ttatctgcgt ctggcgcaac gcattatgca tctgttcagt acgcgtacct
    53281 cttccggcat tttgtatgaa gtggatgcac ggctacgccc gtccggggcc gcgggaatgc
    53341 tggtgacatc cgcagaagca tttgccgatt atcagaaaaa cgaggcctgg acgtgggaac
    53401 atcaggcgct ggtgcgtgcg cgggtagtgt acggcgatcc gcagctcacc gcgcactttg
    53461 acgcggtgcg tcgcgagatt atgacgctgc cgcgtgaagg taaaactctg caaacggaag
    53521 tgcgggaaat gcgcgagaaa atgcgcgctc atctcggcaa taaacatcgc gatcgctttg
    53581 atatcaaagc tgatgaaggc ggaattaccg atatcgaatt tattacccaa tatctggtgt
    53641 tgcgctacgc tcatgaaaaa ccgaagttaa cgcgctggtc agacaacgtg cgcattctgg
    53701 aactactggc gcaaaacgac attatggaag agcaggaagc gatggcgctg acccgtgcgt
    53761 atactacgct gcgcgatgaa cttcatcatc tggcattaca ggaattgccg ggccatgtgc
    53821 cggaggattg cttcaccgca gagcgtgaac tggtgcgggc aagctggcag aagtggctgg
    53881 tggaagaatg aagtatggta ttatcgcgcg caaattttga atctctcagg agacaggaat
    53941 gaaagtaacg ctgccagagt ttgaacgtgc aggagtgatg gtggttggtg atgtgatgct
    54001 ggatcgttac tggtacggtc ccaccagccg tatctcgccg gaagcgccgg tgcccgtggt
    54061 taaagtcaat accatcgaag aacgtccggg cggcgcggct aacgtggcaa tgaacatcgc
    54121 ttctctcggt gccaatgctc gcctggtcgg gctgacgggc attgacgatg cggcgcgtgc
    54181 gctgagtaaa tctctggccg acgtcaacgt caaatgcgac ttcgtttctg taccgacgca
    54241 tccgactatc accaaattac gggtactttc ccgcaaccaa cagctgatcc gcctggattt
    54301 tgaagaaggt ttcgaaggtg ttgatccgca gccgctgcac gagcggatta atcaggcgct
    54361 gagttcgatt ggcgcgctgg tgctttctga ctacgccaaa ggtgcgctgg caagcgtaca
    54421 gcagatgatc caactggcgc gtaaagcggg cgttccggtg ctgattgatc caaaaggtac
    54481 cgattttgag cgctaccgcg gcgctacgct gctgacaccg aatctctcgg aatttgaagc
    54541 tgttgtcggt aaatgtaaga ccgaagaaga gattgttgag cgcggcatga aactgattgc
    54601 cgattacgaa ctctcggctc tgttagtgac ccgttccgaa cagggtatgt cgctgctgca
    54661 accgggtaaa gcgccgctgc atatgccaac ccaggcgcag gaagtgtatg acgttaccgg
    54721 tgcgggcgac acggtgattg gcgtcctggc ggcaacgctg gcagcgggta attcgctgga
    54781 agaagcctgc ttctttgcca atgcggcggc tggcgtggtg gtcggcaaac tgggaacctc
    54841 cacggtttcg ccgatcgagc tggaaaatgc agtgcgtggg cgcgcagata ccggctttgg
    54901 tgtgatgacc gaagaggaac tgaagcttgc tgtagcggca gcgcgtaaac gcggcgaaaa
    54961 ggtggtaatg accaacggtg tctttgacat cctgcacgcc gggcacgtct cctatctggc
    55021 aaatgctcgc aaactgggtg accgtctgat tgtcgctgtg aacagcgatg cctccaccaa
    55081 acggctgaaa ggggattccc gccccgttaa cccgctcgaa cagcgtatga ttgtgctggg
    55141 cgcactggaa gcggtcgact gggtggtgtc gtttgaagaa gacacgccgc agcgcttgat
    55201 cgccgggatc ctgccagatt tactggtgaa gggcggcgac tataaaccag aagagatcgc
    55261 cgggagtaaa gaagtctggg ccaacggcgg cgaagtgctg gtgctcaact tcgaagacgg
    55321 ttgctcgacc actaacatca tcaagaagat ccaacaggat aaaaaaggct aaccggaaag
    55381 cggttcacag atctcgcttc gtagcaggca ggcctattga cgctgttgtt ttttcaacct
    55441 aaagtaaagg aacaagggta agggaggatt tctcccccct ctgatcagtt gttagtaagt
    55501 cgggaaactt aacagtaaca acacaaccag tatgatgacg aacttcatca taaccctttc
    55561 cttctataag gccccttcct cgggaggggc tttcccgttt cagccccttg ctgactcccc
    55621 gattgttgat gagcgccgga ccccgcgcaa cgctttccct gaagcagttt tgccttcccc
    55681 gagcggtcac gcaaaacgct gcaacgtcct gagcatttgc accttttagc ggaacgcaga
    55741 tataaaaaat ccccggcttt ttgggccagg gatatgtgtt aatgaaacct tcttgacgct
    55801 gatttttttt caacctaaag taaaggaaca agggtgaggg aggatttctc ccccctctga
    55861 ttggctgtta ataagctgcg aaacttacga ttaacaacac aatcagtatg atgacgagct
    55921 tcatcataac cctttccttc tgtaaggccc cttcctaggg aggggctttc ccgtttcagc
    55981 cctttgctga ctccccgatt gttgatgagc gccggacctc gcgcaacgct ttcccagagg
    56041 cagttttgcc ttccccgagc ggtcacgcaa aacgttgcaa cgacctgatc atttgcagtt
    56101 tttagcggaa cgcagatata aaaaatcccc ggctttttgt gacagggacg ttcatagctg
    56161 aaaatttatt ctacgttttc ggcgacggga gttgttgagg ttaagggtga agtcaatgcc
    56221 gccagtgagc cgcctgaaac gccaatttca ttgagcaagg agtcaatcag cggtgcctgt
    56281 gtgcggtaag agagagcggc tgataatgct tgttccgcca ggtttccgcc ccccacatta
    56341 cccgtgtttg catcacccgc agcgctgcca cgattcagac catcgacctg aataatcttg
    56401 ataccgtcaa tagatttcat cggctcaacg gatttttcta ttaccgcagg tagcgcctgc
    56461 aacaaggcca gtttgaattt aaggctggtt tgttcatcag aaagtacgtt gatagcatcg
    56521 ttcagcgcac gttgtgcttc tgcttccgcc agaccctttt tacgtgtagc ttcggctaac
    56581 tcaacgatag ccgccgcctg catctctgcg gcttcttttt ctgctttcgc ccgcacggtc
    56641 agttcaaccg ctttggtttc tgcatcctgc gcggcagcga ttagggcaac ttgtttagca
    56701 cgatcggctt ctgcggtctg gcgagtggtt tctacgtttt gttgcgcact tactgcttct
    56761 gcgagtgcaa ggttagcacg cgcttctgcc tgggactgtt gttccgattt ggcggcaata
    56821 gcgatcgatt tcgtctggtt ggcgatttcg gtgacctgct gttgttcgat ctctttaata
    56881 cgaacttcac gttcagcctc aacctttctt gagcggacgg cctgctcgcg ctcgatctcc
    56941 gtttcctgaa tttgtcgttc agccagaatt cgcgtctact ctgcttcacg acgacgttca
    57001 gcttcaaaag ccgcaatacg tgcattctgt tcggcagtac gggttttaac ctgctgctcc
    57061 tgctcaagcg tcataaacgc ttcttgttgt tcaatctcca acttgcgcga aagtgcatca
    57121 cggtttttct cacgcaccgc aacttctaca tcctgttcaa cttcgttacg ttcgcgacga
    57181 cggcgctccg tctcctgagt cagtttggtt aaaccttcgg cgtcaaaggc attgttcgga
    57241 ttgaaatgtt ctttcgaggt ctggttaaag ttggtaagtg aaacgctttc cagctcaaga
    57301 ccgtttttcg acaggtcttc tgcgacagta ttttgtactc cctgcacgaa gttctcgcgg
    57361 gtatcctgta actcatgcat ggtcatttgt gcagctgttg cacggagggc atcgacaaat
    57421 ttatcttcaa ccaacatacg taagtcttct ggtgatagcg tgcgttgccc caacgtctgc
    57481 gccgcagtgg cgatgccttc tacagacggt tttacccgta caaagaaagc gactactaca
    57541 tcgacacgca tacgatcttt cgtaatcagg ctatcaatgg ttgagcggct gacttccagc
    57601 ttcagagtat tcatattgat ggggattatt tcatgaaaga tcggcatcac gattgcgcca
    57661 ccgctcatca ccactttttg cccacctaaa ccagtacgaa caaaagcttg ctctgccgaa
    57721 gcgcgacgat agagcctggc gaaaataatt ccaataataa acagaatgca tacggcaata
    57781 atcgcggtaa acat
//
LOCUS       DAZFRJ010000009        53318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000009 DAZFRJ010000000
VERSION     DAZFRJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.009"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            337..2730
                     /gene="malP"
                     /locus_tag="ACHKUN_000487"
     CDS             337..2730
                     /gene="malP"
                     /locus_tag="ACHKUN_000487"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO6658801.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKMIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2740..4824
                     /gene="malQ"
                     /locus_tag="ACHKUN_000488"
     CDS             2740..4824
                     /gene="malQ"
                     /locus_tag="ACHKUN_000488"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO6658802.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4869..6185)
                     /gene="gntT"
                     /locus_tag="ACHKUN_000489"
     CDS             complement(4869..6185)
                     /gene="gntT"
                     /locus_tag="ACHKUN_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6658803.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6546..7121)
                     /gene="nfuA"
                     /locus_tag="ACHKUN_000490"
     CDS             complement(6546..7121)
                     /gene="nfuA"
                     /locus_tag="ACHKUN_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO6658804.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7180..7863)
                     /gene="gntX"
                     /locus_tag="ACHKUN_000491"
     CDS             complement(7180..7863)
                     /gene="gntX"
                     /locus_tag="ACHKUN_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO6658805.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7901..8671
                     /gene="bioH"
                     /locus_tag="ACHKUN_000492"
     CDS             7901..8671
                     /gene="bioH"
                     /locus_tag="ACHKUN_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO6658806.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8700..9578)
                     /gene="rpnA"
                     /locus_tag="ACHKUN_000493"
     CDS             complement(8700..9578)
                     /gene="rpnA"
                     /locus_tag="ACHKUN_000493"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO6658807.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(9781..10017)
                     /gene="feoC"
                     /locus_tag="ACHKUN_000494"
     CDS             complement(9781..10017)
                     /gene="feoC"
                     /locus_tag="ACHKUN_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO6658808.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(10017..12338)
                     /gene="feoB"
                     /locus_tag="ACHKUN_000495"
     CDS             complement(10017..12338)
                     /gene="feoB"
                     /locus_tag="ACHKUN_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO6658809.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12355..12582)
                     /gene="feoA"
                     /locus_tag="ACHKUN_000496"
     CDS             complement(12355..12582)
                     /gene="feoA"
                     /locus_tag="ACHKUN_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO6658810.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(13020..15341)
                     /gene="yhgF"
                     /locus_tag="ACHKUN_000497"
     CDS             complement(13020..15341)
                     /gene="yhgF"
                     /locus_tag="ACHKUN_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO6658811.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15438..15914)
                     /gene="greB"
                     /locus_tag="ACHKUN_000498"
     CDS             complement(15438..15914)
                     /gene="greB"
                     /locus_tag="ACHKUN_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO6658812.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            16142..16861
                     /gene="ompR"
                     /locus_tag="ACHKUN_000499"
     CDS             16142..16861
                     /gene="ompR"
                     /locus_tag="ACHKUN_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO6658813.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            16858..18210
                     /gene="envZ"
                     /locus_tag="ACHKUN_000500"
     CDS             16858..18210
                     /gene="envZ"
                     /locus_tag="ACHKUN_000500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO6658814.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(18286..19908)
                     /gene="pckA"
                     /locus_tag="ACHKUN_000501"
     CDS             complement(18286..19908)
                     /gene="pckA"
                     /locus_tag="ACHKUN_000501"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO6658815.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKELGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            20287..22011
                     /gene="yhgE"
                     /locus_tag="ACHKUN_000502"
     CDS             20287..22011
                     /gene="yhgE"
                     /locus_tag="ACHKUN_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO6658816.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(22146..23024)
                     /gene="hslO"
                     /locus_tag="ACHKUN_000503"
     CDS             complement(22146..23024)
                     /gene="hslO"
                     /locus_tag="ACHKUN_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO6658817.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23049..23450)
                     /gene="hslR"
                     /locus_tag="ACHKUN_000504"
     CDS             complement(23049..23450)
                     /gene="hslR"
                     /locus_tag="ACHKUN_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO6658818.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(23461..24129)
                     /gene="yrfG"
                     /locus_tag="ACHKUN_000505"
     CDS             complement(23461..24129)
                     /gene="yrfG"
                     /locus_tag="ACHKUN_000505"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO6658819.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(24194..26329)
                     /gene="igaA"
                     /locus_tag="ACHKUN_000506"
     CDS             complement(24194..26329)
                     /gene="igaA"
                     /locus_tag="ACHKUN_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO6658820.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            26649..27209
                     /gene="nudE"
                     /locus_tag="ACHKUN_000507"
     CDS             26649..27209
                     /gene="nudE"
                     /locus_tag="ACHKUN_000507"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO6658821.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(27375..29927)
                     /gene="mrcA"
                     /locus_tag="ACHKUN_000508"
     CDS             complement(27375..29927)
                     /gene="mrcA"
                     /locus_tag="ACHKUN_000508"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO6658822.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            30047..30826
                     /gene="hofM"
                     /locus_tag="ACHKUN_000509"
     CDS             30047..30826
                     /gene="hofM"
                     /locus_tag="ACHKUN_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO6658823.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            30826..31365
                     /gene="hofN"
                     /locus_tag="ACHKUN_000510"
     CDS             30826..31365
                     /gene="hofN"
                     /locus_tag="ACHKUN_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO6658824.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            31349..31789
                     /gene="hofO"
                     /locus_tag="ACHKUN_000511"
     CDS             31349..31789
                     /gene="hofO"
                     /locus_tag="ACHKUN_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO6658825.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            31779..32183
                     /gene="hofP"
                     /locus_tag="ACHKUN_000512"
     CDS             31779..32183
                     /gene="hofP"
                     /locus_tag="ACHKUN_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO6658826.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            32095..33333
                     /gene="hofQ"
                     /locus_tag="ACHKUN_000513"
     CDS             32095..33333
                     /gene="hofQ"
                     /locus_tag="ACHKUN_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO6658827.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            33734..34255
                     /gene="aroK"
                     /locus_tag="ACHKUN_000514"
     CDS             33734..34255
                     /gene="aroK"
                     /locus_tag="ACHKUN_000514"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO6658828.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            34312..35400
                     /gene="aroB"
                     /locus_tag="ACHKUN_000515"
     CDS             34312..35400
                     /gene="aroB"
                     /locus_tag="ACHKUN_000515"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO6658829.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            35492..36778
                     /gene="damX"
                     /locus_tag="ACHKUN_000516"
     CDS             35492..36778
                     /gene="damX"
                     /locus_tag="ACHKUN_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO6658830.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            36885..37721
                     /gene="dam"
                     /locus_tag="ACHKUN_000517"
     CDS             36885..37721
                     /gene="dam"
                     /locus_tag="ACHKUN_000517"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6658831.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            37739..38416
                     /gene="rpe"
                     /locus_tag="ACHKUN_000518"
     CDS             37739..38416
                     /gene="rpe"
                     /locus_tag="ACHKUN_000518"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO6658832.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKLSHE"
     gene            38409..39167
                     /gene="gph"
                     /locus_tag="ACHKUN_000519"
     CDS             38409..39167
                     /gene="gph"
                     /locus_tag="ACHKUN_000519"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO6658833.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            39160..40164
                     /gene="trpS"
                     /locus_tag="ACHKUN_000520"
     CDS             39160..40164
                     /gene="trpS"
                     /locus_tag="ACHKUN_000520"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO6658834.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            40433..41338
                     /gene="yhfZ"
                     /locus_tag="ACHKUN_000521"
     CDS             40433..41338
                     /gene="yhfZ"
                     /locus_tag="ACHKUN_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO6658835.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            41355..41717
                     /gene="yhfY"
                     /locus_tag="ACHKUN_000522"
     CDS             41355..41717
                     /gene="yhfY"
                     /locus_tag="ACHKUN_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO6658836.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            41801..42964
                     /gene="yhfX"
                     /locus_tag="ACHKUN_000523"
     CDS             41801..42964
                     /gene="yhfX"
                     /locus_tag="ACHKUN_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO6658837.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            42964..44190
                     /gene="yhfW"
                     /locus_tag="ACHKUN_000524"
     CDS             42964..44190
                     /gene="yhfW"
                     /locus_tag="ACHKUN_000524"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6658838.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            44187..45065
                     /gene="php"
                     /locus_tag="ACHKUN_000525"
     CDS             44187..45065
                     /gene="php"
                     /locus_tag="ACHKUN_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO6658839.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            45076..45429
                     /gene="yhfU"
                     /locus_tag="ACHKUN_000526"
     CDS             45076..45429
                     /gene="yhfU"
                     /locus_tag="ACHKUN_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO6658840.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            45441..46745
                     /gene="yhfT"
                     /locus_tag="ACHKUN_000527"
     CDS             45441..46745
                     /gene="yhfT"
                     /locus_tag="ACHKUN_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO6658841.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            46757..47842
                     /gene="yhfS"
                     /locus_tag="ACHKUN_000528"
     CDS             46757..47842
                     /gene="yhfS"
                     /locus_tag="ACHKUN_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6658842.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(47994..48725)
                     /gene="frlR"
                     /locus_tag="ACHKUN_000529"
                     /gene_synonym="yhfR"
     CDS             complement(47994..48725)
                     /gene="frlR"
                     /locus_tag="ACHKUN_000529"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO6658843.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(48825..49610)
                     /gene="frlD"
                     /locus_tag="ACHKUN_000530"
     CDS             complement(48825..49610)
                     /gene="frlD"
                     /locus_tag="ACHKUN_000530"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO6658844.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(49607..50437)
                     /gene="frlC"
                     /locus_tag="ACHKUN_000531"
     CDS             complement(49607..50437)
                     /gene="frlC"
                     /locus_tag="ACHKUN_000531"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO6658845.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(50487..51509)
                     /gene="frlB"
                     /locus_tag="ACHKUN_000532"
     CDS             complement(50487..51509)
                     /gene="frlB"
                     /locus_tag="ACHKUN_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO6658846.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(51530..52867)
                     /gene="frlA"
                     /locus_tag="ACHKUN_000533"
     CDS             complement(51530..52867)
                     /gene="frlA"
                     /locus_tag="ACHKUN_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO6658847.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(53162..53317)
                     /gene="yhfL"
                     /locus_tag="ACHKUN_000534"
     CDS             complement(53162..53317)
                     /gene="yhfL"
                     /locus_tag="ACHKUN_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO6658848.1"
                     /translation="MKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIG
                     GCGPTAQ"
BASE COUNT        12536 a        13477 c        14673 g        12632 t
ORIGIN      
        1 tcttaataaa tctattaaga atgagatgga gcacactgga tattttactt atgaaactgt
       61 ttcactcctt tacttaattt atagagttac cttccgcttt ttgaaaatac gcaacggcca
      121 ttttttgcac ttagatacag attttctgcg ctgtattgca ttgatttgct gctaatcctg
      181 tggtttgcac tagctttaag tggttgagat cacatttcct tgctcatccc cgcaactcct
      241 ccctgcctaa tcccccgcag gatgaggaag gtcaacatcg agcctggcaa actagcgata
      301 acgttgtgtt gaaaatctaa gaaaagtgga actcctatgt cacaacctat ttttaacgat
      361 aagcaatttc aggaagcgct ttcacgtcag tggcagcgtt atggcttaaa ttctgcggct
      421 gaaatgactc ctcgccagtg gtggctagca gtgagtgaag cactggccga aatgctgcgt
      481 gctcagccat tcgccaagcc ggtggcgaat cagcgacatg ttaactacat ctcaatggag
      541 tttttgattg gtcgcctgac gggcaacaac ctgttgaatc tcggctggta tcaggatgta
      601 caggattcgt tgaaggctta tgacatcaat ctgacggacc tgctggaaga agagatcgac
      661 ccggcgctgg gtaacggtgg tctgggacgt ctggcggcgt gcttcctcga ctcaatggca
      721 actgtcggtc agtctgcgac gggttacggt ctgaactatc aatatggttt gttccgccag
      781 tcttttgtcg atggcaaaca ggttgaagcg ccggatgact ggcatcgcag taactacccg
      841 tggttccgcc acaacgaagc actggatgtg caggtaggga ttggcggtaa agtgacgaaa
      901 gacggacgct gggagccgga gtttaccatt accggtcaag cgtgggatct ccccgttgtc
      961 ggctatcgta atggcgtggc gcagccgctg cgtctgtggc aggcgacgca cgcgcatccg
     1021 tttgatctga ctaaatttaa cgacggtgat ttcctgcgtg ccgaacagca gggcatcaat
     1081 gcggaaaaac tgaccaaagt tctctatcca aacgacaacc atactgccgg taaaaagctg
     1141 cgcttgatgc agcagtactt ccagtgtgcc tgttcggtag cggatatttt gcgtcgccat
     1201 catctggcgg ggcgtaaact gcacgaactg gcggattacg aagttattca gctgaacgat
     1261 acccacccaa ctatcgcgat tccagaactg ctgcgcgtgc tgatcgatga gcaccagatg
     1321 agctgggatg acgcctgggc cattaccagc aaaactttcg cttacaccaa ccataccctg
     1381 atgccagaag cgctggaacg ctgggatgtg aaactggtga aaggcttact gccgcgccat
     1441 atgcagatta ttaacgaaat taatactcgc tttaaaacgc tggtggagaa aacctggccg
     1501 ggcgatgaaa aagtgtgggc caaactggcg gtggtgcacg ataaacaagt gcatatggcg
     1561 aacctgtgtg tggttggcgg tttcgcggtg aacggtgttg cggcgctgca ctcggatctg
     1621 gtggtgaaag atctgttccc ggaatatcac cagctatggc cgaacaaatt ccataacgtc
     1681 accaacggta ttaccccacg tcgctggatc aaacagtgca accctgcact ggcggctctg
     1741 ttggataaat cactgcaaaa agagtgggct aacgatctcg atcagctgat caatctggaa
     1801 aaattcgctg atgatgcgaa attccgtcag caatatcgcg agatcaagca ggcgaataaa
     1861 gtccgtctgg cggagtttgt gaaagttcgt accggtattg agatcaatcc acaggcgatt
     1921 ttcgatattc agatcaaacg tctgcatgag tacaaacgcc agcacctgaa tctgctgcat
     1981 attctggcgt tgtacaaaga aattcgtgaa aacccgcagg ctgatcgcgt accgcgcgtc
     2041 ttcctcttcg gcgcgaaagc ggcaccgggc tactacctgg cgaagaatat tatctttgcg
     2101 atcaacaaag tggctgacgt gatcaacaac gatccactgg ttggcgataa gttgaaggtg
     2161 gtgttcctgc cggattattg cgtttcggcg gcggaaaaaa tgatcccggc ggcggatatc
     2221 tccgaacaaa tttcgactgc aggtaaagaa gcttccggta ccggcaatat gaaactggcg
     2281 ctcaatggtg cgcttactgt cggtacgctg gatggtgcga acgttgaaat cgccgagaaa
     2341 gtcggtgaag aaaatatctt tatttttggt cataccgtgg aacaagtgaa ggctattctg
     2401 gccaaaggct acgacccggt gaaatggcgg aagaaagata aggtgctgga cgcagtattg
     2461 aaagagctgg aaagcggtaa atacagcgac ggcgataagc atgccttcga ccagatgctg
     2521 cacagtatcg gcaaacaagg cggcgatccg tatctggtga tggcggattt cgcagcctat
     2581 gttgaggcac aaaagcaggt ggatgtgctg taccgcgacc aggaggcctg gactcgcgcg
     2641 gcgatcctca ataccgcccg ctgcggtatg tttagctcgg atcgctctat tcgcgattat
     2701 caggctcgta tctggcaggc aaaacgctaa ggaagctcga tggaaagcaa acgtctggat
     2761 aatgccgcgc tggcggcggg gattagcccc aattacatca atgcccacgg taaaccgcag
     2821 tcgattagcg ccgaaaccaa acggcgtttg cttgacgcga tgcatcaacg taccgccacg
     2881 aaagtggcgg taacgccagt cccgaatgtc atggtttata ccagcggcaa aaaaatgccg
     2941 atggtggtgg agggcagcgg cgaatatagc tggctgctga ccaccgaaga aggaacacag
     3001 tacaaaggcc atgtaacggg gggcaaagcg ttcaatctac cgacgaagct gccggaaggt
     3061 tatcacacgc tgacactcac ccaggacgac cagcgcgcgc attgccgggt gattgtcgcc
     3121 ccgaaacgct gttacgaacc gcaggcgttg ctgaataaac aaaagctgtg gggtgcctgc
     3181 gttcagcttt atacgctgcg atcggaaaaa aactggggta ttggggattt tggcgatctc
     3241 aaagcgatgc tggtggatgt ggcaaaacgt ggcgggtcgt tcatcggcct gaacccgatt
     3301 catgcgctct atccggcaaa tccggagagt gccagcccat acagcccgtc ttctcgccgt
     3361 tggctgaatg tgatttatat cgacgttaac gccgttgaag atttccatct tagcgaagag
     3421 gctcaggcct ggtggcagtt gccgaccacg caacagacgc tgcaacaggc gcgcgatgcc
     3481 gactgggtcg attactccac ggttaccgcc ctaaaaatga cagcattacg aatggcgtgg
     3541 aaaggtttcg cgcaacgtga tgatgagcag atgaccgcgt ttcgccagtt tgttgcagag
     3601 cagggcgaca gcctgttctg gcaggcagcc tttgatgcgc tacatgccca gcaagtgaaa
     3661 gaggacgaaa tgcgctgggg ctggcctgca tggccagaga tgtatcagaa cgtggattca
     3721 ccagaagtgc gtcagttctg cgaagaacat cgtgatgacg tcgattttta tctctggttg
     3781 cagtggctgg cttacagcca gtttgccgcc tgctgggaga taagccaggg ctatgaaatg
     3841 ccgattggct tgtatcgtga tctggcggtt ggcgtagcgg aaggtggggc ggaaacctgg
     3901 tgtgaccgtg aactatattg cctgaaagcg tcggttggcg cgccgccgga tatcctcggc
     3961 ccgttggggc agaactgggg attaccgcca atggacccgc atatcatcac cgcgcgtgcc
     4021 tatgaaccgt ttatcgagct gttgcgtgcc aatatgcaaa actgcggcgc attacgaatt
     4081 gaccatgtga tgtcgatgct gcgtttgtgg tggataccgt atggcgagac ggcagatcag
     4141 ggcgcgtatg ttcactatcc ggtggatgat ctgctctcga ttctggcact cgaaagtaaa
     4201 cgtcatcgct gtatggtgat tggtgaagat ctcggtaccg taccggtaga gattgtcggt
     4261 aagctgcgca gcagcggtgt gtactcctac aaagtgctct atttcgaaaa cgaccacgag
     4321 aagacgttcc gtgcaccgaa agcgtatccg gagcagtcga tggcggttgc ggcgacacat
     4381 gacctgccta cgctacgcgg ttactgggag agcggggatc taacgctggg caaaaccctg
     4441 gggctgtatc cggatgaagt ggtactgcgc ggtctgtatc aggatcgcga actggcgaag
     4501 caagggctgc tggatgcact gcataaatat ggttgtctgc caaaacgtgc cgggcataag
     4561 gcatcgttga tgtcgatgac gccgacgctg aaccgtggtt tgcagcgcta cattgccgac
     4621 agtaacagtg ctctgttagg actacagccg gaagactggc tggatatggc cgaaccggtg
     4681 aatattcctg gcaccagtta ccagtataaa aactggcgac gcaagctttc cgcaacgctt
     4741 gagtcgatgt ttgccgatga tggcgtgaac aagttgctga aggatttgga cagacggcgc
     4801 agagctgcag cgaagaagaa gtagagtgcg gttgatgccg gatgcgatgc atccggcagt
     4861 cagtgttatc aaatcaccat attcagcagc agacagccta ccagaccgca caccgagata
     4921 atggtttcca gcatcgacca ggatttgatg gtctcgccga tagtcaggtt aaagtactct
     4981 ttgaacagcc agaagcccgg atcgttcaca tgagagaaaa tcacactacc ggaacccacc
     5041 gcaataacca tcagctccgg gctaacaccc gtcgttgcaa tcagcggtgc cgcgatacca
     5101 cccgcagtga ttgccgcaac ggttgcggaa cccagcgcga tacgcagtac ggcagcaatc
     5161 gaccaggcca tcagcagcgg agaaatgttg gtttcgtgca tcatggaagc aatgtatttg
     5221 tccacgccgc tgtctaccag cacctgcttg aacgcaccgc caccaccgat gatcaacagc
     5281 atcatcgcaa tgattttgat ggaagaaacc agcgtgtcgt taatctgatc cattgaacga
     5341 ccacggttca gaccaaaggt gaacatggca atcagcacgg caatcagcgt agccattacc
     5401 gggtcaccga ggaactccgc taccggcagg aaagcgtgac ctttcggcag gatcatttcg
     5461 gcaatcgcac gcatcgccat cagtactacc ggcaccagag aggtccagac gctgacgcca
     5521 aagctcggca tctcttcttc gctgaaggtt ttcgcgctgt agagaccttc cggaattggc
     5581 ttatcgatac ctttcaacac gcgagcgtaa accggaccgg cgagaatcac ggtcgggatt
     5641 gccagaatag taccgtacag cagggtttta cccatatcgg cattgaaaat ggtggcaatc
     5701 gcagtcggac ccggatgcgg tggcaggaag ccgtgggtca cagacagtgc agctgccatt
     5761 ggtacaccaa catacaacag tggaatattc gcagaagccg cgatggtaaa caccagcggc
     5821 agcatcagca caaagcccac ttcatagaac agggcaaaac caacggtaaa accggtcagt
     5881 accaccgccc actggatgtg ttttttacca aatttggcaa tcagcgtggt ggcgatacgt
     5941 tgtgcgccac cgcagtctgc cagcattttg cccagcattg cgccaaaacc catgatcagg
     6001 gcaaggctac cgagcgtccc gccgacaccg gctttgatgg agccaataac tttatccagc
     6061 ggcattcctt gcattaatcc aacagcaagc gccacgagga cgagagcgat gaagccgttc
     6121 attttgaagc ggatcatcag gagcaacaac aagattacac cgatagcaac aatgactaat
     6181 ggcatgattt acctggcctt tcatttgtta tgggtaacgt caattttctg acgacaaact
     6241 ctaattatcc caatcgggaa cagagatatt gcggcaccac gactgatacc cactaaaact
     6301 aattattgta gtcagatgtc aggagtatgt ttggtaccca tgtgaatgat acgggtaaca
     6361 tctggcgttt gagaatcacc agagcggggt aaatttaaat tatgagaggt tggtcatatt
     6421 atcgcgggga aacgaaggga gggatttgcc aaagcaatgc tgtgccaacg tctggcacat
     6481 gttcaacgta ggcccgaaat gacgctttag cgtcgcatcg ggcaatctac aaaagagggg
     6541 ataacttagt agtaggagtg ttcgccgcgc tggtgttcgg tgagatcgcg cacacctttc
     6601 agctccggga attcgttcag cagctgcttc tcgatccctt ctttcagcgt cacatcgacc
     6661 atggaacaac cgttacagcc gccgccaaat tgcagaatgg cgtaaccgtc ttcggtgatt
     6721 tccatcagcg aaacgcgacc accgtgacca gcaagctgtg ggttgatctg cgactgcagc
     6781 atatactcca cgcgctccat cagcggtgca tcgtctgcca ctttacgcat tttggcgttc
     6841 ggggctttca gcgttaactg agaacccaac tggtcggtaa caaagtcgat ctctgcatct
     6901 tccaggtatg gtgcgcttaa ctcatcaaca tacgcggtca gcaggtcaaa tttcagggca
     6961 gtgtcggtgg cttccacggc atccggcgga caataagaaa caccacattc agcgttaggc
     7021 gtgccagggt taatcacaaa tacgcggatt tgtgtccctt cttcctgatt tgccagcagt
     7081 ttggcaaagt gcgcttgtgc agcatcggaa atacggatca tagtaatggc ctaatagttg
     7141 actattttag ttggttataa tacgcccatc atcgaggctc tacaaggttc gacaaaggca
     7201 ccagacctgg acagtcgccg caccattgcg taaaagcaac tgcgcaatct ctgcgacggt
     7261 acttccggtg gtaacgacat catccacaat caccatatgg cgaccttgca cgggcaattc
     7321 aagacgaaag gcatttttca ggttgcgctt gcgcagccgg gcactgagaa aatgctgggt
     7381 cgcagtggcc cgtgtacgtg tgacggcttc gctatcccat tggcaatgca accagcgtga
     7441 taacggctga cacagcaaat cgctctgatt aaatccccga cgccagtgac gccgctgcca
     7501 taacggaacg ctgatgatgc gatccggcaa ttgcaacccg gtggtgcgac gagcgtgtaa
     7561 gacttccaat agtaacagac gtgacagggc gctggcgatt tcactgcgcc gggaaaattt
     7621 aagctggtgg ataagcggac ttaacggcgg cgcatagtcg gcaaccgtga ccagtctttg
     7681 ccagggcggc ggtttttgca ggcagcgacc gcagggaaga tgggagtgtg tggcgggtaa
     7741 tccacattgt gggcataacg ttttatctgt gcgggtggcg cgtgaacaga ccgaacaaat
     7801 cccccaatga cctaacgcca gtggcattcg gcatagccag cataatcccg gtactgttag
     7861 catatgttca tccttgtaag tcaaaagaga acaatagcgg atgaataaca tctggtggca
     7921 gaccaaaggt caggggaatg ttcatcttgt gctgctgcac ggatggggac tgaatgccga
     7981 agtgtggcgt tgcattgacg aggaacttag ctcgcatttt acgctgcacc ttgttgacct
     8041 gcccggcttc gggcgtagcc ggggatttgg tgcgctgtca cttgctgata tggccgaagc
     8101 cgtgctgcaa caggcacctg ataaagccat ttggttaggc tggagtctgg gcgggctggt
     8161 ggcaagccaa attgttttaa cccatcccga gcgtgttcag gcgctggtca ccgtggcgtc
     8221 gtcaccttgt tttagtgctc gtgacgagtg gccggggata aaaccggacg tactggcggg
     8281 atttcagcag caactcagtg atgattttca gcgtacagtg gagcggttcc tggcgttaca
     8341 aaccatgggg actgaaacgg cgcgccagga tgcgcgggcg ttgaagaaaa ccgttctggc
     8401 gttaccgatg ccggaggttg acgtgcttaa tggcgggctg gaaatcctga aaacggtcga
     8461 tctccgtcag ccgctgcaaa acgtgtccat gccgtttttg cgattgtatg gctatctcga
     8521 cggtctggtg ccgcgcaaag tggtgccgat gctggataaa ctttggcctc acagcgaatc
     8581 atatatcttc gccaaagcgg cccatgcgcc gtttatttcg catccggtcg agttttgtca
     8641 cctgctggtg gcgttgaagc agagggtgta ggcaactttt gaaatggcga gacagaaatt
     8701 tactggctcg ccgcagccaa ctcttcttct gacaccccgg taaagcgcat gatgtctgta
     8761 agagggactc cggattcaag cattattttg gctatatgca gggctttgga ttgttccccc
     8821 tcctgccgca atctttccgc aattgtcatt aaactctcct tgtgtttcgg tgaacgttcg
     8881 gcaacgccgt cgataaaatc gttaaaacgt accgcgtcgc cggtttgcag tatgtaatta
     8941 aacagccctt tgatttgtct gtcattagcg tatccactac ttaataagca ggccatttgc
     9001 tctaccagcc ccatcagatc gcgttgacga atatgttttt gaattaactc cagcagcgcc
     9061 atacgtcggt gctgcatgat ttcatcatca ggcatgacgg tgacatcaat cagcggaaat
     9121 gcggaggcat aaagctgcct tgccagtttg ggatcggcga aacaatccag ccaacacagc
     9181 gaatagggat aggggctttc aataccgtgg taaaacaaca atggcaccac catcggcaac
     9241 gttttgtatc cagcatcaag gtgattttgc attgcggcaa tagcgtaacg catcatgcga
     9301 aatgcgatca gtttgtttga ggtgctttga tgttcaatca gacaatagat gtatcctggt
     9361 ccttgttccg ttttcaccga ccacagcaca tcggaatagc tttcacgcag atcgtcatca
     9421 ataaagctgc tcgactccag cttgagggtt ttcatatcac aaagcgcgtg aatgggtgcc
     9481 ggtaaatgaa acgcaagaaa atcacgagcc gtgtccggtt ggcgtaaaaa gagtttgaac
     9541 agcgcatcgt gtggggtgga actctgcttt ttgctcatgg cgttccttca cctgttgttt
     9601 gaatgccgcg actctacgcg ccactccaca cctgtgtatt agcgagtttt catccctgcg
     9661 agatgctttc aaaggaactt acgccgtgct gcaagagttg catttttatg gaaagccgca
     9721 cgcaaaaaag cccggatgca gacgcatacc gggctttcca aaacaggcga tggagtaagg
     9781 ttaacgcagc gcccaccact cgcgcagaca ggcttttcct tccgggcagc ttttacaact
     9841 gccagagagg cagccgtcag gttcttcctg aatccgcacg gctttgccca tactttccag
     9901 ttgttgcagc atggcgttaa tcattggctg tggagtgttc aatgtctggc ttatctgggc
     9961 cgcttccata cggccccgta acgccagcaa atcgcgcacc tgaataagtg aagccattaa
    10021 tggcaatcac cggtggtgct ggctgcgcag caactgctta ccgacttgcg ggttgccagc
    10081 agttcgatat ccacccggct acgcgcgcgg cgcagcagac cgataaccac gatgttaaac
    10141 aggataaccg ccagaatgca caccaggctg taagttgggt gctgactgta gctggcgacc
    10201 tgatagaaca atgttgccag cgagtaagcg atattcagcc cccacaggat ggagaagccc
    10261 atccagccac ggcttgattc acgggcgatg gctcccatca ccgagataca tggtacatac
    10321 agcaggacga aaatcaggta gctgtaagct gctgctgcgc taccgaattt ctgatccatc
    10381 acgcccatcg ccccggtacc catttcgccg tcgcctttgc tggcttcaat ggggttcatc
    10441 agtacgctaa ggctgaaggt gtctttcagg ctctgccagg tttcatctat cgcactgaac
    10501 agctcttcac cgaggttaaa ctctgccgga ttgaactctt cgtcctgaat attttctgcg
    10561 gtgtagaggg tgttgagcgt acccactacc acttctttcg ccatggcacc tgtaaacagg
    10621 ccaaccgttg cctgccagtt atcttcatgc acgccaattg gcttgaagac cggggtgatc
    10681 acccggctga cggacgccag cgccgagtcg ttgatgttat cgacgatttt cccgctcagc
    10741 gagaagctgt tgaaagcgct caggaaaatg ctgacgatga tgatcacttt accagcacgc
    10801 agaacgaagc ctttcagacg ctgccaggtc tggataatca ggcttttaac gtgtggtaca
    10861 tgatagaccg gcaactccat gacaaacggc gtcgcttcac cgcgcatgat ggtgtacttg
    10921 agcatcaggc cagtcagcac cgccatcaca atacccagca tatacagcga gaagaccgcc
    10981 agcgcaccgt tctgcccgaa gaaggcagcc gcgaatactg cgaagatagc cagacgcgcg
    11041 ccgcaggaca taaacggtgc catcatgatg gtcatcagac gttcacgcgg tgcatcaagc
    11101 gtacgtgcac ccattaccga cggtacgtta caaccgaaac cgacgatcag cggcacaaag
    11161 gatttccccg gcaagcccag cgcctgcatc agacggtcca tcacaaacgc cgcacgcgcc
    11221 atatacccgg agtcctcaag gaaggagagg aacaggtaca tcatgccaat ctgcggcacc
    11281 agtggcagca cggtgttaat gccgccaccc aggccctggg cgaggaagat agtcagccag
    11341 tccgggaagt ggagcgtgta gccaatccat tgaataccat gcacaaatag cgccacggag
    11401 ccgacgtcaa acagcggctg taacgccccg ccgatgttga tagccagcag gaacatcagg
    11461 tacatcacaa agaggaaaat cggcagaccg aggaaacggt tgagcacgat tttatctacc
    11521 gcagtggtga aacggctggg ttctgccgtc agggtgttgc ttaccacatc acagatggca
    11581 gcaatgcact ggtaacgcgc atcggcaatg tgcagcgccg gatcgtccat ctcattacgc
    11641 agacgggcga gggcggcatc cagatgctgc gacgcttcac cggcgtaggc gcggctgtag
    11701 atatcgcctt ccagcatttg caggcccagc cagcgacgtt gtttcagcgg gatgtcggaa
    11761 ggcatcactt ttgccagtga atctgcttcg ttgagtagcg gctgtgcgta atgcaccagt
    11821 tccacattct cgttagcttt atagcgatca atcgccagct tgagcgcttc aataccgcga
    11881 ccacgggttg aaaccagcgg gatcactggg cagccaagac gcgccgacag agcatcaatt
    11941 tcaatacgaa tattttgctt ctcggcaatg tcgagcatgt tcagtgccac gatgcaggga
    12001 atgccgagtt ccagcagttg tagcgtcagg tacaggttac gctcaaggtt agacgcatcc
    12061 accacgttaa tcagcaggtc ggcgtcgcca ctcaaaatgt agtgacaggc gatttgctca
    12121 tcgagcgagg tctgcgatga gatggtggtc agagaatagg tgccgggcag gtccaccagc
    12181 gtgacctgat gatcggtggt ggagaattgc ccttctttac gttcgacggt aacgccagcc
    12241 cagttaccta cacgctgacg tgcgccagtg agctggttaa ataaggttgt cttgccagaa
    12301 tttggattac caattaagcc tatggttaat tttttcattg ttgttatcac cgtattaaca
    12361 ggaaaccgct tccacttcta ataaggccag atcttttttg cgtaatacca ggctcacacg
    12421 acgggtttcg atatgaatgg ggtcgccgag tggagcgacg cgcaccacat taaaagagga
    12481 gccaggtaac atgccaagag aaagcagttt ttggcgatat gccgggctga tttcacggga
    12541 aaagccagtg attttccacg cagtatctgg agtgtattgc ataggtgcct acttgtttct
    12601 cattaactgg ataactacct catggggcgt ttgacagcga catgagacca gatgatttgc
    12661 caacgaagga gagattaaat aatcaacagc acaatgataa tgagaatggt ttctatcagc
    12721 aatacttaaa atgtgtgcga atgttggttt gaataataat ctgactcaat gttgatgtgg
    12781 ctcaaggtta tatcgcagcg aaaatataat tcgaatagcg aattaccagg actggctaat
    12841 gtttaattaa ggtttcagct gttaatgaag tgataaatgt tgccggaata tattgtgagt
    12901 ttggctgtgg aataaaaatg ccggagatat tctccggcac ttttatgtca gacaaggcgt
    12961 tcgttgccag atgcggcgtg gacaccttat cgcgcaggtt taggggtcaa tcagggcgtt
    13021 taacgttttt tgcccattgc cgccgccagc gcatccatca tcgcgctatt accggcaggc
    13081 tgcgcttcac gaccgcgtgg tttggctgcc gggcggttgt tttgcgggcg ttcattaccg
    13141 ccgccgcgac gggcgttggt ttcgccaggc tgctcgtcca gacgcatggt cagggcgata
    13201 cgtttacgct gaagatccac ttccagcact ttcaccttca caatgtcgcc cgctttcacc
    13261 acggtatgcg gatcttccac aaacttgttc gacaatgaag agatgtgaac caggccgtcc
    13321 tgatgcacgc caatatcgac aaacgcgcca aagttggtga cgttggtgac agcgccttcg
    13381 aggatcatac ccggttgcag gtcgttcatt gtctcgacgc catcggcaaa ctgagcggtt
    13441 ttaaattccg gacgcggatc gcgacccggt ttttccagct ctttgatgat gtcagttact
    13501 gtcggcacac cgaatttttc atcagtaaag tcagacgctt tcaggttacg cagttcgctg
    13561 ctgttaccca tcagatcttt cagtgcctgc tgtgttgctg ccagaatgcg ttccaccacc
    13621 ggataggctt ccgggtgaac ggtagaggcg tccagcgggt tatcaccgtg gttaatgcgc
    13681 aggaagcccg cgcactgctc gaaggctttc ggccccagac ggctgacttt cagcagttgc
    13741 tgacggttct tgaactggcc gttctcatcg cgccaggcca cgatgttttg cgccatcatg
    13801 cgcgtcaggc ccgccacgcg ggttaatagc ggaacagaag cggtgttgag atcgacgcca
    13861 acggcgttta cgcagtcttc tactactgca tccagtttgc gggccagttg cgtctggctg
    13921 acgtcatgct gatactgacc tacgccgata gatttcggat cgattttcac cagctccgcc
    13981 agcggatcct gcaaacggcg ggcgatagat accgcaccac gcagcgaaac gtcgagatcc
    14041 gggaactcct gcgctgccag ctcggaagcc gagtaaaccg acgcgccagc ttcgctgacg
    14101 atcactttct gcgcggtcac tttcgggaac tgcttctgca cgtcgagata gaaacgttcg
    14161 gtttcgcggg aagctgtacc gttgccgatc gccaccagtt caacgttatg cttttcacac
    14221 aaggcagcaa cggtcatcgc tgcttttgct gtttgtccgg tgtgcgggta aatggtatcg
    14281 gtcgccacca gtttgccagt ggcatcgacc accgctactt ttaccccggt acgcagaccc
    14341 ggatcgaggc ccatcgtggc acgcagtccg gcaggggccg ccatcagcag atcgtgcagg
    14401 ttacgggcaa agacgttgat tgcttcatct tccgcgcgtt cgcgcacggt gcccatcagt
    14461 tcggtttcca gatgcatcag caccttgatg cgccaggtcc agctcaccac gcctttgcgc
    14521 cagctatccg ccggagcatt gttcaggcgc aggccgagat gatccatgat gatttgctcg
    14581 caatagctct cttttggcgg ctcttcgaac tgcggatcgg cattcagcga aagctggagt
    14641 acgccttcgt tacgcccacg gaacatcgcc agcgcgcggt gagaaggcac cgtggacagc
    14701 ggttcgtgat gatcgaaata gtcgcggaat tttgcccctt cctcttcttt accgctcatc
    14761 acggttgaga ccagatgcgc gttcttccac agataatcac gcactttcgc cagcagcgcg
    14821 gcatcttcgg caaaccgttc catcaggata tagcgcgcgc cgtccagcgc ggctttggta
    14881 tctgccacgc ctttatctgc atcaacatat tgtgcagcgg cgacttctgg cgtgtgtgac
    14941 ggatcgctcc acagcaggtc agccaacggc tcaagccctg cttcaatggc gatttgcccg
    15001 cgggtgcggc gtttaggttt gtagggcagg tagagatctt cgagttcggt tttgcttagt
    15061 gtggcgttga tggccttcgc cagatcatcg gtgagtttgc cttgctcgga aatggatttg
    15121 aggatcgcct gacgtctctc ttccagctcg cgcagatagc tcagacgcgt ttccagatta
    15181 cgcagctgcg tgtcatccag accgccggtg atttccttac gataacgtgc gataaacggc
    15241 acggtattcc cttcgtcaag caggcgaacg gcagcgtcaa cctgttccgg gcgcgcctga
    15301 atttcacccg caataatgcg gcagaacgaa tcattcatca tggtttgagt tcatcttttc
    15361 ggatcaaaaa tcaggggata gttatacgga ctggctggca aaaatgccag ccatcggcag
    15421 gaggttaaga ctcttcctta cggtttcacg tactcgatag cattgacata ccagctcgct
    15481 tccccggcag gggtattcac caccgccaga tcgccgactt cttttttcag caatgcgcgg
    15541 gccatcgggg aatcgataga gatgtaatct ttacggccaa aaatttcatc gtagccgaca
    15601 atacggaaac ggtgagtcac gccatcgtcg ttttcaatct ccacccatgc gccaaaaaag
    15661 accttgcctt cctgctgagg ggagtaatcg acgattttga gattttccat gcatttagtg
    15721 agatagcgca cgcgacggtc gatttcacgc agacgctttt tattatactg atagtcagca
    15781 ttttcgctgc ggtcgcccag acttgcggcc caggtcacct tttttgtgac ctccgggcgt
    15841 tcttcacgcc agagataatt aagctcttgt ttgagttttt catacccttc ccgggtaacc
    15901 aggggcgttt tcatctcgtt gattcccttt gtctgtttga taatgcgcac attgggtata
    15961 acgtgatcat atcaacagaa tcaataatgt ttcgccgaat aaattgtata cttaagctgc
    16021 tgtttaatat gctttgtaac aatttaggct gaaattcata ccagatttag ctggtgacga
    16081 acgtgagctt ttttaagaat acacgcttac aaattgttgc gaacctttgg gagtacaaac
    16141 aatgcaagag aactacaaga ttctggtggt cgatgacgac atgcgcctgc gtgcgctgct
    16201 ggaacgttat ctcaccgaac aaggcttcca ggttcgaagc gtcgctaatg cagaacagat
    16261 ggatcgcctg ctgactcgtg aatctttcca tcttatggta ctggatttaa tgttacctgg
    16321 tgaagatggc ttgtcgattt gccgacgtct tcgtagtcag agcaacccga tgccgatcat
    16381 tatggtgacg gcgaaagggg aagaagtgga ccgtatcgta ggcctggaga ttggcgctga
    16441 cgactacatt ccaaaaccgt ttaacccgcg tgaactgctg gcccgtattc gtgcggtgct
    16501 gcgtcgtcag gcgaacgaac tgccaggcgc accgtcacag gaagaggcgg taattgcttt
    16561 cggtaagttc aaacttaacc tcggtacgcg cgaaatgttc cgcgaagatg agccgatgcc
    16621 gctcaccagc ggtgagtttg cggtactgaa ggcactggtc agccatccgc gtgagccgct
    16681 ctcccgcgat aagctgatga accttgcccg tggtcgtgaa tattccgcaa tggaacgctc
    16741 catcgacgtg cagatttcgc gtctgcgccg catggtagaa gaagatccag cgcatccgcg
    16801 ttacattcag accgtttggg gcctgggcta cgtctttgta ccggacggct ctaaagcatg
    16861 aggcgattgc gcttctcgcc acgaagttca tttgcccgta cgttattgct catcgtcacc
    16921 ttgctgttcg ccagcctggt gacgacttat ctggtggtgc tgaacttcgc gattttgccg
    16981 agcctccagc agtttaataa agtcctcgcg tacgaagtgc gtatgttgat gaccgacaaa
    17041 ctgcaactgg aggacggcac gcagttggtt gtgcctcccg ctttccgtcg ggagatctac
    17101 cgtgagctgg ggatctctct ctactccaac gaggctgccg aagaggcagg tctgcgttgg
    17161 gcgcaacact atgaattctt aagccatcag atggcacagc aactgggcgg cccgacggaa
    17221 gtgcgcgttg aggtcaacaa aagttcgcct gtcgtctggc tgaaaacctg gctgtcgccc
    17281 aatatctggg tacgcgtgcc gctgaccgaa attcatcagg gcgatttctc tccgctgttc
    17341 cgctatacgc tggcgattat gctattggcg ataggcgggg cgtggctgtt tattcgtatc
    17401 cagaaccgac cgttggtcga tctcgaacac gcagccttgc aggttggtaa agggattatt
    17461 ccgccgccgc tgcgtgagta tggcgcgtcc gaggtgcgtt ccgttacccg tgcctttaac
    17521 catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta
    17581 agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag
    17641 caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt
    17701 gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt
    17761 aatgcagtac tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc
    17821 gcgctttacc ccggcagcat tgacgtgaaa atgcacccgc tgtcgatcaa acgcgcggtg
    17881 gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt cagcagcgga
    17941 acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa
    18001 caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc
    18061 acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag
    18121 cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg
    18181 cgggcgcagg gcacgacaaa agaagggtaa ataaacggga ggcgaaggtg cctcccgttt
    18241 tgctttctat aagatactgg atagatattc tccagcttca aatcattaca gtttcggacc
    18301 agccgctacc agcgcggcac ccgcaggggt gtcggtgtat ttatcgaagt tgtcgataaa
    18361 cagtttcgcc agggtttcgg ctttttcctg ccactgttcc ggagaagcgt aggtgttacg
    18421 cggatcgaga atcttcgtgt ctacgcccgg cagttcggtt gggatcgcca ggttaaacat
    18481 cggcagagtg aaggtttctg cattatccag cgaaccgttg aggatggcgt cgataatggc
    18541 gcgggtatct ttaatcgaga tacgtttgcc agtgccgttc cagccagtgt taaccagata
    18601 agcctgcgcg cccgccgcct gcatacgttt caccagcact tctgcgtact gagtcgggtg
    18661 cagcgacagg aacgccgcgc cgaagcaagc ggagaaggtt ggcgtcggtt cggtgatgcc
    18721 acgctcagta ccggccagtt tggcggtgaa gccagagagg aagtgatact gggtttgatc
    18781 ggcagtcagg cgagaaaccg gcggcaacac gccgaaagca tcagcagtca ggaagataac
    18841 cttagtcgcg tggcccgctt tggaaaccgg cttaacaatg ttatcgatgt gatagatcgg
    18901 ataagaaacg cgggtgttct cggtttttga accatcatca aagtcgatag tgccatcttc
    18961 acgcacggtg acgttttcca gcagcgcatc gcgacggata gcgttgtaga tttcaggttc
    19021 cgcttctttc gacagcttga tagtttttgc gtagcagccg ccttcgaagt taaacacgcc
    19081 gtcatcgtcc cagccgtgct cgtcatcacc aatcaggcga cgtttcgggt cggtggaaag
    19141 ggtggtttta ccggtgccgg aaaggccgaa gaacaccgca acatcgcctt tctcaccaac
    19201 gttggcggag cagtgcatag aagcgatacc tttcagcggc agcaggtagt tcatcatcga
    19261 gaacatccct ttcttcattt cgccgccgta ccaggtgccg ccaatcagct gcatgcgctc
    19321 ggtcaggtta aacgccacga agttttcgga gttcagaccc agttctttcc actgcgggtt
    19381 agtgcacttc gcgccgttca taacgataaa gtctggtttg aaacctgcca gctcttcatc
    19441 gctcgggcga ataaacatgt ttttgacaaa atgcgcctgc caggccactt cggtgatgaa
    19501 acggacggaa agacgagtat ccggattcgc accacagaaa gcgtcgacaa cgaacagacg
    19561 tttgccggaa agctgcctgg tcaccaggcc tttcagatgc tgccaggttt ccggagagag
    19621 gggtttgttg tcgttcttac ctttgccttt gtctgcccac cagaaagtat cgcgagtggt
    19681 atcgtcacgg acgatatact tatcttttgg tgaacgaccg gtgaagatcc cggtatcgac
    19741 ggcaacggca cccagattag ttaacacccc gcgctcataa cctgtcaggc tcggatcgag
    19801 ctcttcctga tacagcaggt cgtagcttgg gttgtaaacg atatcatgta cgtcactgat
    19861 accataagcc tcgagttctt gcggggtcaa accattgtta acgcgcattt cactgctcct
    19921 tagccaatat gtattgcctg aatagtaaag tctttttggg ggtgttaacc gcgacaaggc
    19981 tcatagattt acgtatctgg agaaattcat atttgacaaa aaactccgtg attcctgtca
    20041 cgaaacggtt gctattatcg cagaaatagc attttaggtg tggaaaatgt tctcaaaagg
    20101 ttaaatcttg gtttatatgg cgggaatatt gtgagtggaa tcgcattcct taatgaaatt
    20161 gatgcaaatt caatcggtaa agtatcgatt caccacgcta actttttttg attattttct
    20221 gaaaaaagca aaatactact gcccggatgc gataattaac ccaccttttg attctggata
    20281 accaacatgg ataacgttga actttcaccg gcaacacgct gggggatgat tgctaccgga
    20341 ttacttcagg ggctggtttg ctacctgttg atcgcctggc ttgccggaaa aaatcacagt
    20401 tggattgttt atggcgtgcc tgcgaccgtt gcgttttcat ccgttttact cttctccgtg
    20461 atctctttta aacagaagcg cctttgggga tggctggcgc tggtgtttat tgccacgaca
    20521 gggatgagcg gttggctgaa gtggcaaact gatggcatga acccctggag agctgaaaag
    20581 gcgatttggg attttggctg ctatctgctg ttgatggcaa tgttgttgct accgtggata
    20641 caacaaagcc ttcgtatccg caatggcagt agccgctata gctactttta caagtcagta
    20701 tggcataacg tacttatatt actggtgatt tttctcgcca atggcctgac gtgggtggtg
    20761 cttttactgt ggggtgagtt gtttaaactt gttggcatca agtttttcaa tacgctcttt
    20821 tttgcaaccg actggtttat gtatcttaca ttaggtctgg ttaccgcact ggcggtgatt
    20881 ctcgcgcgca cacagtcacg tttaatcgac tctattcaaa agttgttcac gttaatcgcc
    20941 acggggttgc tgccgttagt atcattgcta accctgatgt ttatcatcac tctgccgttt
    21001 acgggcctga gcgcgatttc tcgccacatc tccgccgccg ggttgctgtt aacgctggcc
    21061 tttttgcaat tgatcttaat ggctattgtc cgcgatccgc aaaaagcgtc acttccctgg
    21121 acggggccgt tgcgttgcct gattaaaacc gctttgctgg ttgctccgct gtatgtgttc
    21181 gtcgccgcct gggcgttatg gctgcgggtc gctcagtacg gctggactgt cgaccgcttg
    21241 cagggcgcgc tggcggtgct ggtgttactg gtgtggtcgc tggggtattt tgtcagtatc
    21301 gtctggcgta atgggcaaaa tccccttgtt cttcagggca aagtgaacct tgcggtttcg
    21361 ttattggtgt tggtgatact ggtgcttctt aattcgccgg tgctggatag tatgcgtatt
    21421 agcgtgaaca gtcatatggc gcgttatcag agcggcaaaa acacgccaga ccaggtaact
    21481 atctacatgc tcgaacagag cggtcgctat ggacgtgcgg cgcttgagtc gctgaaaagc
    21541 gatgccgggt ttatgaaaga cccgaaacgc gcgcgggatc tgctgatggc gttagatgga
    21601 gagcaacatc ttcaggaaca ggtatcggaa aaagtattag ccgaaaatgt gttaattgcc
    21661 cctggttctg ttaaaccgga tgcgacattc tggtcggcct taatccagga tcgctataac
    21721 gtgatgacct gtattgaaaa agacgcctgc gtcctggtcg agcaagatct gaatagtgat
    21781 ggtcaggcgg agcggatcct gtttgctttt aatgatgaca gagtcattgt ctatggcttt
    21841 gactcagaca gaaaagaatg ggacgcgctt gatatgagtt tacttccgaa cgaaataacg
    21901 aaagaaaaat tactcacagc tgcgaaggat ggaaaactgg ggacgaggcc gaaagcgtgg
    21961 cgcgatctga cggtagatgg tgaaacgctg gaaataaact taagtaagtg acaactcttt
    22021 taacggatac gacgctgtat ctgaccaaca ataatttgcg atttatttca caggcctggt
    22081 aagcgcagca catcaggcca ttacgtcgta aaatccggca gagccctctc tgccggacat
    22141 actcattaat gaacttgcgg atctgccgga gacgcgttgt tgcggatttc agcaatatcc
    22201 atcgcattga acagatagtg gttaccgcag taatcacaat gcatgtcaat ttcgccatct
    22261 tccgtcagga tgctatcaac ttcttcatca ggcagcgttt tcagcgcatc ggcgcaacgt
    22321 tcacgcgagc aggtgcattt gaactccaca tcctgcggat cgtaaaccgt cacctcttct
    22381 tcgtgataca aacgccacaa cacttcgttt gccggtaagg tcagcagttc ttcggttttg
    22441 atggtttcgg ttagcgtcgc caggtggtca aagtcgtcct gctgggcatt ttgcgcaggc
    22501 attacctgca acaacatacc gcctgcagcc ggtttgccgt ctacgtcgcc ggtgcgaata
    22561 aacaggcgcg tcggcagctg ttcagaacgc ataaagtaat cttccaggca ggccgccagg
    22621 gtatcacctt ccagaccaac cacgccctga tagcgttcgc cttcgctcgg ggtaatggtg
    22681 atcaccacgt aaccattgcc gaccagcgtt ttcaggtcgg cattttctgg aatttcgccc
    22741 tgcacgcgcg ccacaccgcg catttgctgg ttattgttac cgttaataac cgccagattc
    22801 atcggaccgt cgccttgcag ctgtacggtg atatcaccat caaacttcag cgtagcggtt
    22861 aacaggctgg tcgcaaccag cagttctgcc agcacgtttt taacgggctg cggataatcg
    22921 tggttctcaa ggatctgttg cagggtttcc gaaacggtta ccagttcgcc gcgaacggca
    22981 aaattttcaa acagatagcg atgtaattgg tcatgttgcg gcataatcat ctctcttgca
    23041 ggtgacagtt attcactgtc gccgtgttta aatcgtaaca ggtcgcggcg ctcttttttg
    23101 tccgggcgtc ggtccgggtg cggcatggtt aaggcattaa gtttacgtgc catcgccatt
    23161 ttttcgcgtt tctctacact ttccgcagtc tcttcataca gcaaggctgc ctcgctggcg
    23221 gggcgacgct gttcagtaat cgcctttaca atcaccgtgc gttcgtcatt tccctggcgc
    23281 agagtgagcg tggcattcag ctcgacgatt ttgctcggct tgctgcgctg cccgttgtaa
    23341 tgcaccttac cgccttcaat catttcacgg gccagcgcgc gggttttata aaaacgggca
    23401 gcccatagcc atttatccag tcgaacctca acagcaggtt tctctttcat ggcgtctcct
    23461 tcacattagc gaggggatca ggcggcggta gtcattcagt gacggatggc gttgatactg
    23521 tttctcggca atcccggaat caggattagt cacgccgagg cagtaacgaa taccaaattg
    23581 cgcggcagca tcgagaatcg cttcgctgtc atcaataaac agcgttcttt cagctttcag
    23641 acccgtagct tcggccaccg catgccataa ccgctgatcc tctttcggat aaccaaatgt
    23701 gtgggtggaa agtaataaat caaggtgtgc gtccagaccg gtatgctcaa gttttaccgc
    23761 caggttgtgc ggatgcgcat tggtgagcaa aattcgctgc ttaccgctgg ctttcagtgc
    23821 ctcaagaaac ggaatggtat cttcacgcag tacggcacgc ggtcccatct cggtggtcat
    23881 cgcacagata tccagaccca gttgctcact ccagtaatca agacagtacc agtttagcgt
    23941 atgctgtacg tcgtgatatt gctggcgcat atattccatc gcttcctgtg gcgtaacccc
    24001 gtttttggcg ccccatgttt caggcaccag cttttgccag aaatagttat cgaaggcgag
    24061 gtcgagcaac gtgccgtcca tatccagcag aacggtatct acgtcctgcc aggcaatgtt
    24121 gatatgcatg agggaaatct ccagagtgaa gcaatttgcg cgacagggta gcataacctg
    24181 ccgcgcaaac gtgttattcg ataaggcttt ctgaaggggt gatcagttgc gggttcaggc
    24241 agctttcata ataggcctga atctccatca tgcgagtgcg atgacgctgg taacgacgcc
    24301 aggcctgtac gccattgtaa atggcgctac ccagcatcgt cagtagcagc aatgtggtgc
    24361 tgagatagcg ccacagtccg gaacgatccg ggatcggatg aaggccaata tgctgcgtac
    24421 cattggcgtc agtgaagatt tttgtgacga taccttcggc gttaaacggc gtatgcatca
    24481 gcatttgtgc cagtttctgg aaagcgttcc actgttcttg cggcgggtag tcgtaaagtg
    24541 atgccgaagg ccagggctga tcaacaaaat cgctgccttc gtcgctgaca atcaggaatc
    24601 cgccgggggc cggactgttt agtgattgtg ccgctcgcgc cgtttcatgc gtgataaacg
    24661 gcgcggtgga ggttgccacc aggttatcca gcgattctgc actcaccggg cgtaataaca
    24721 cattcacgcc atcgagcttc ccggcgttgg cgcgttttac cagcgcgtcc cagtctttac
    24781 tgttgccgag attgaccagc gcatttttca gtcgcacaca gtcatctttg gcagaacata
    24841 aatccgctgt cttcagtaca atgtcgccaa aatcatcaag caataccatg ccggattttt
    24901 gaatcgctga acgtaatgag gcactgacgc gagattcatc ttccggttta gggtgcagct
    24961 ggcgattaac tgcttcagtc aatgccgtcg ctttgttgac cagttcagat tctggtaatg
    25021 gtaatgagcg ggcgtcattc cagatgatct gcgagcagtc aaacggtaaa aaaggtgaat
    25081 tggttttcgc gctccaggtt ccggaagtgc gaatattaca cattcccgta ccgctaatac
    25141 gcaatgtatc gcctacccgc acgccagcgt cagccagttg ttttacgctg gtggcttcaa
    25201 tggtctgcgc gcctttcatc catgagagag tgaatttcag cggcatatcc agcgggaccc
    25261 agaataacag cataaacagc accagcagcg agcccgcagc gataatcgta ctgcgcagcc
    25321 agtgctgtaa cggaaagttt tttacttcat catgcagcga aagatatcgc ccctgacgca
    25381 ctacgtggcg gtcgagatag atatcaatat cggtttgttg accgagatcc tgagcaatgt
    25441 atggctgcca gtgtgcggga tagaccaggt cgataatacc gagtgaaata ttgttgatct
    25501 gttcctgatc gttttcgcca aacaatcccc aacgacgggg tgtaccgcgc agacaatgaa
    25561 tttctcgcag ggaggatttt gccgggggcg cgaatagccc ccacagacct gcgcccagca
    25621 gcagtaatgc gccgcctgcc agccacggaa caaatacatc cggggtaatc aggcagaaaa
    25681 agaacatcag gaaggaggcg acgatcagca acgcttcacg cagcccgcgc ggacgactca
    25741 gcgcatattc ttcatgcgtt tctttgcgaa tattgagtag ctcgatttgc tcactctctt
    25801 ccccgcgaat cgacgcctgc gtggacggaa caggttgcaa ggcatagccg cgcgtttcct
    25861 gcatgtactc ctgcagcgta tgaccgttga gtgaaataac cagcggcagc gaatcggtat
    25921 aaatcagttc aacggtattc tcatcgttga tgtattgttc ccagaaaggg ggcaggtgga
    25981 cttctaccga atcgaggtag taacgccatt tattcggatc gtcggtagag atgccgtaac
    26041 gcgtgatagc gtgtgtcagc atcatgacgt tgttgctttc ggcattcagc gccagagaga
    26101 tcggtgccgc gctggctccc gttgggccag gcacctgtaa tacctgcgtc aggctctcaa
    26161 gataattttc aacggcgcta cgttcttcag gtgtgagttt acgcgtttgc gcatccgcga
    26221 aggcgttggt ccagggcagc tgacgccgtc tggatcgcac ttttatcagc catcccgcaa
    26281 gtagtgagca ggccagcaaa gcagctaaaa aaatcacaat ggtgctcatg ctttccccat
    26341 cttacttagt ctgtcaggcg tggtcagtcg caccagacac aatacatgat tctgcggttg
    26401 gctatcggca agcgtggagt ttaacttgag catcattaag cacatcacag taaccgctga
    26461 tcatagcaaa ccctctgaag gcggaatatc agcaaattcc tggagttatt tcaacctatt
    26521 gacttttaat ttgatttgag gacgattatt tattaagtta cataaatgta aataacatgc
    26581 aattcacatt gccgaaagcg tgtccgatat cgcacaataa ccgctgattt cacagcatag
    26641 atcccacgat gagcaaatca ttacaaaaac ccaccattct gaatgttgaa actgtagccc
    26701 gttcccgact gtttaccgtc gagagcgtgg atctggagtt cagcaatggc gtgcggcgtg
    26761 tttatgaacg aatgcgtcca accaaccggg aagcagtgat gattgtgccg attgtggacg
    26821 atcacctgat cctgatccgc gaatacgcag tgggaactga atcctacgaa ttaggttttt
    26881 cgaaaggatt aattgatccg ggtgaaagcg tctacgaagc cgctaaccgc gagctaaaag
    26941 aagaggttgg atttggtgcg aacgatctga cttttttgaa gaagctcagc atggcaccgt
    27001 cttacttttc cagcaaaatg aatatcgtgg tcgcgcaaga tctctacccg gaatcgctgg
    27061 aaggcgatga gccagagccg ctaccacagg tgcgctggcc gctggcgcat atgatggatt
    27121 tgctggaaga ccctgacttc aacgaagcgc gtaatgtcag cgcgctgttc ctcgtgcgcg
    27181 aatggttgaa agggcagggg cgagtgtaaa tgcgtgaagt ttgcctgatg cacaacgctt
    27241 atcaggctta tgggtttcct gcaatatatt gattttttca cgattttgtt agccggataa
    27301 cgcgttcacc cgcatccggc ataaacaaat gcactttgtc agccaactga aaaggcgccg
    27361 aagcgccttt ttaatcagaa caattcctgt gcctcaccat tatcgataat ggtcgttccc
    27421 acctcgtgca ccgcctgttg tgtcggctgc gtaccttcga tgaaatactc ttcgcggctg
    27481 ttgccaccat tagctaactg cccggtgctg cgatcgatat tcaccgtcac aatacccgga
    27541 ggcggcgtca gcggctgctc cggcacacct tcaagaacgg ctttcatata agcgtcccat
    27601 gcaggctggg cactcttggc accgccttcg taacctgaga tctgatcttt aatcgctccg
    27661 gaagccgttg tatgaccgag attacgacgg tgatcatcaa agccaatcca gaccgaggtc
    27721 acaacgcccg gaccgtaacc cgagaaccac gcatctttcg aactgttagt ggtaccggtt
    27781 ttcccgccga tatcgcgacg ctgcaaatca cgacctgcac gccagccggt accctgccag
    27841 cctggttcac caaagatatt ggtgttcaaa gcactcttaa tcaggaatgc cagcggagtg
    27901 ttgatgacgt gcggtgcgta ctcctgcgcg ccagtcttcg ccactaacgc ctgatttgcc
    27961 tgctccagct gcggcattgg tacagaaaca ttctgctgct cgcgggagat agcgacatct
    28021 tcaacatcgt tattttccag cacgttcgat ttctgcgtat caccgtaaat caccggaata
    28081 tcgcattccg ggcaggctac tttcggtttc gcttcgaaaa tcacgccgcc ctgatcgttt
    28141 tcaattttgc tgataaacca cgggtccacc aggaagccac cgttcgccat gaccgcgtag
    28201 ccgcgcgcca cctgcattgg ggtgaaggac gctgaaccca gcgccagcga ttcggtgtgg
    28261 acaatgtttt gcgccgggaa gccgaagcgt tgcagatatt ctgcagcgta gtcgacgccc
    28321 atcgcccgca ttgcgcgtac catcaccacg tttttcgact gacccagccc ctgacgtaag
    28381 cgaattggac cagcatactg cggtggtgag ttcttcggct gccagtcaga accggcacct
    28441 gcatcccagc gagaaattgg cacatcgttc aacatacttg ccagcgtcag acctttatcc
    28501 atcgccgcgg tgtagaggaa cggtttgatg ttggaaccca cctgacgcag tgcctgggtg
    28561 gcgcggttaa acttgctctg attgaaatca aagccaccga ccagcgccat aacggcaccg
    28621 ttttgcggat tgatcgacac cagcgccgag ttcacttccg gcacttgtgc cagccaccat
    28681 gcatcgccaa cctgacgaac ccagatttgc tgacccgttt gcagaacatc ggtcacttta
    28741 cgcggcgtcg gtccttgctg agtatccgaa cggtaaggac gcgcccagcg aacgccttcc
    28801 atactcaatg cgacggtcga cccgtccgcc agcatcgccg tcgcttgctg aggattggcg
    28861 ctggtgactg cggcaggcag cagcggacca taggttggca gcgccttcag cgtatcggta
    28921 atcttgttgt tatcccacgc cgactcgccc actttccaca gcacatttgc cgggccgcga
    28981 tagccgtggc gcatgtcgta gtccagcacg ttattacgta ccgcctgctg tgcggcctgc
    29041 tgcactttgc gggtgatggt ggtgtaaatg cgataaccgt cttcataggc actttcgcca
    29101 taacggttat acatctcctg gcgcaccatt tcgctcaggt acggcgcaga gaaagcaatc
    29161 tccggcgcgt gatagttagc gttaatcgcc tcagtgcgtg tctgatcgaa ctgttgttgg
    29221 gtgatatacc cttcatccag catccgcgac agtacgacgt tacgccgcgc gacggcacga
    29281 tccattgagt agagcggatt aaaggtggaa ggcgctttcg gcagcccggc gatcacagcc
    29341 atttcgttca gcgtcagttg gtcgaccgtt ttaccgaaat agacttgtgc cgcagcaccg
    29401 acaccatagg cgcggtaacc gaggtaaatc ttgttcagat aaagctcgag gatctcgtct
    29461 ttcgtcagca actgttcaat gcgaatcgcg aggaagactt ccttaatctt acgcatcagc
    29521 gtgcgttctg gactgaggaa gaaatttctc gccagctgct gggtaatggt actcgcccct
    29581 tgtgacgcgt ggccggagaa cagcgccaca cttgccgcac ggaagatccc caccgggtca
    29641 acgccgtgat gctcgtagaa gcggctgtct tccgtcgcga taaaggcttt caccatctcc
    29701 ggtgggattt gatccaacgt aaccggaata cgacgtttct caccgtattg agcaatcagc
    29761 tcgccatcgg cgctgtaaat ctgcatcgga atttgcaggc gaacatcttt taatgtcgcc
    29821 acatccggca gttgtggctc gatgtagcgg tataggccat aaatcgagcc tgctcccagc
    29881 agaatgcaac agactgcaag gatcaaaaaa tactttacga acttcactgg aaatttccca
    29941 tttagtttca tttgggcagt ttataaacaa acgcgcggta gtataaaggc aagccagacg
    30001 cattgatata cccgtcagag tgacgggtga taaggagagc atcacaatgg catttaagat
    30061 ctggcaaatt ggtttgcatt tacaacagca agaagcggta gcggttgcga tcgtgcgggg
    30121 agcaaaagaa tgctttttgc aacgctggtg gcggttgccg ctggagaacg acattatcaa
    30181 agatgggcgg attgttgatg cgcagcagct ggctaaaacg ttgttaccgt ggagtcgcga
    30241 actgccgcag cgtcatcaca ttatgttggc gtttcccgcc agtcgcacat tacagcggtc
    30301 atttccgcgc ccgtcgatgt cccttggtga gcgggagcaa acggcctggc tgtcagggac
    30361 gatggcccgc gagctggata tggatccgga ctccctgcgt ttcgattaca gcgaagactc
    30421 actcagtccc gcctataacg tgactgccgc gcaaagcaaa gagctggcaa cgctgcttac
    30481 tctggcagaa aggttgcgtg ttcatgttag tgcgatcacc ccggatgcca gtgcattaca
    30541 gcgattcctg ccttttttac cttctcatca gcaatgtctg gcctggcgtg ataacgaaca
    30601 gtggctgtgg gcgacgcgct atagctgggg gcgcaaactg gcggtgggga tgactagcgc
    30661 gaaggagctg gcggcagcgt tatccgttga tcccgacagc gtcgcgatat gtggcgaagg
    30721 cggatttgac ccctgggagg ccgtttctgt tcgtcagccg ccgctaccgc cgcctggtgg
    30781 agactttgcc atcgcgctgg ggctggcgct tgggaaggcg tactgatgaa cccgccaatt
    30841 aattttttgc cctggcgaca gcaacgccgg accgcttttc tgcgtttctg gttgctgatg
    30901 ttcgttgcgc ctctgctgct ggccgtcggg ataacgctaa tactgcgtct gacaggcagc
    30961 gccgaagcgc gcatagacgc agttttgctt caggcggaac aacaactcgc ccgcagctta
    31021 cagataacga agccacgttt gctggagcaa cagcagttac gcgaacagcg ttcgcaacgg
    31081 cagcgccagc gacaatttac ccgcgactgg caatctgcgc tggaagcact ggcggcgctt
    31141 ttacccgagc acgcctggct gacaacgata agctggcagc agggaacgct ggagatcaag
    31201 gggctgacaa caagcattac cgcgttaaac gcactagaaa cgtcactccg ccaggatgcg
    31261 tcttttcatc tcaatcagcg gggagccacg cagcaggatg cgcagggacg ctggcaattt
    31321 gagtatcagt taacaaggaa ggttagcgat gaacatgttc tttgactggt ggttcgccac
    31381 atcaccccgc ctccgccagt tttgctgggc attctggttg ctgatgttag ttacgctcat
    31441 ttttctgtca tcgacacacc atgaagagcg cgacgcatta attcgactac gggcaagtca
    31501 tcaccagcag tgggccacac tgtatcgcct ggttgacacc actcccttca gcgaagaaaa
    31561 aacgctgccc ttttcgccac tggattttca gttgcccggc gcgcaactgg tttcctggca
    31621 tccatccgcg cagggaggtg agttggcgtt gaaaacgctg tgggaagcag tgccgtcggc
    31681 atttacacgg ctggcagagc gcaacgtcag cgtgagccgt ttttcgttaa gcgtggaagg
    31741 tgatgatctt ttgttcacgc tacaactgga gacaccgcat gagggttaaa cgctggttgt
    31801 tggcaggtat tgcattgtgc cttttaaccg gtatgcgtga cccttttaaa ccgccggaag
    31861 atctatgccg gattagcgaa cttagccagt ggcgctatca ggggatggta gggcgaggcg
    31921 agcacatcat cggtgtaata aaagacgggc aaaagaaatg gcgacgggtg cagcaaaacg
    31981 atgtgctgga aaacggctgg acaattttac agctgacgcc agacgcacta acgctgggaa
    32041 ccgggacaaa ctgcgaaccg ccacaatggt tgtggcaacg gcaaggagat acaaatgaag
    32101 caatggatag ccgcactact gttgatgctg atacccggcg tacaggcggc aaagccgcaa
    32161 aaagtgacgc tgatggtgga tgacgttccg gtagctcagg tgttgcaggc gctggctgaa
    32221 caggagaagt tgaacctggt cgtgtcgcca gacgtcagcg gtacggtgtc gttacatcta
    32281 acagatgttc cctggaagca ggcactacaa actgtagtga aaagcgccgg actgataacg
    32341 cggcaggaag acaacattct ctcagtgcat tccattgcct ggcagaataa caatatcgcc
    32401 cgccaggagg cggagcaggc gcgggcgcag gcaaatctgc cgctggaaaa tcgcagtata
    32461 accctgcaat acgccgacgc gggagaactg gcgaaagcgg gggagaagct actgagtgcc
    32521 aaagggagta tgaccgtcga taaacgcacc aatcgccttt tgctacgaga taacaaaacg
    32581 gcgttaagcg cgcttgaaca gtgggtagcg caaatggatc tgccggtcgg gcaggttgag
    32641 ctgtcggcgc atattgtcac cattaatgaa aaaagtttgc gtgagttagg cgtgaaatgg
    32701 acgctggccg atgcgcaaca cgctggtggc gttgggcaag tcaccacgct tggtagcgac
    32761 ctctccgtag cgacggcgac aacgcatgtc ggttttaaca ttgggcgcat caacggacgc
    32821 ttgctggatc ttgagctttc tgcgctcgaa caaaaacagc agctggatat tatcgccagt
    32881 ccgcgtctgc tggcctcaca tcttcagcct gccagcatta aacaggggag cgaaattcca
    32941 tatcaggttt ccagcgggga aagtggcgcg acgtcggtgg aatttaaaga ggccgtcctg
    33001 gggatggagg tcacgcccac ggtgttacaa aaaggtcgca tccggctgaa attacacatc
    33061 agccagaacg ttccggggca ggtgctacag caggccgatg gcgaagtgct ggcgattgat
    33121 aagcaggaga tcgaaacgca ggtcgaggtc aaaagcggag aaacgttggc gctgggcggc
    33181 atttttaccc gtaaaaataa atcgggtcag gatagcgtac cgttgcttgg cgacattccc
    33241 tggttcgggc aattatttcg tcatgacgga aaagaagatg aacgacgcga gttagtggtg
    33301 tttatcacgc cacgactggt ttccagtgag taaacagccg taaaagcggt aatgttttta
    33361 tgctgaacgt gtttcatcta tttgacgcgc gcaggtattt agcatacaag gagtaccgat
    33421 ttgagagttg gtgctcttcg ctgcctgcgt tccatgatga tgatttatca ttcaggcggc
    33481 attttgctgt cttttttacg ctaatcttac ccggtgattt atcgccagag cggtggtagc
    33541 aaggcagcgc gcttgcagcg accagatatg cagagggatg ggtgatttat tcagttgcca
    33601 aacccgctgg agtattgaga taattttcag tctgactctc gcaatatctt atgaggtttc
    33661 agttcatgtc ctgcggcgct ctctgagcga agcgggttta tcattaacga atagtcttag
    33721 tagtaccgaa aaaatggcag agaaacgcaa tatctttctg gttgggccta tgggtgccgg
    33781 aaaaagcact attgggcgcc agttagctca acaactcaat atggaatttt acgattccga
    33841 tcaagagatt gagaaacgaa ccggagctga tgtgggctgg gttttcgatt tagaaggcga
    33901 agaaggcttc cgcgatcgcg aagaaaaggt catcaatgag ttgaccgaga aacagggtat
    33961 tgtgctggct actggcggcg gctctgtgaa atcccgtgaa acgcgtaacc gtctttccgc
    34021 tcgtggcgtt gtcgtttatc ttgaaacgac catcgaaaag caacttgcac gcacgcagcg
    34081 tgataaaaaa cgcccgttgc tgcacgttga aacaccgccg cgtgaagttc tggaagcgtt
    34141 ggccaatgaa cgcaatccgc tgtatgaaga gattgccgac gtgaccattc gtactgatga
    34201 tcaaagcgct aaagtggttg caaaccagat tattcacatg ctggaaagca actaattctg
    34261 gctttatata cactcgtctg cgggtacagt aattaaggtg gatgtcgcgt tatggagagg
    34321 attgtcgtta ctctcgggga acgtagttac ccaattacca tcgcatctgg tttgtttaat
    34381 gaaccagctt cattcttacc gctgaaatcg ggcgagcagg tcatgttggt caccaacgaa
    34441 accctggctc ctctgtatct cgataaggtc cgcggcgtac ttgaacaggc gggtgttaac
    34501 gtcgatagcg ttatcctccc tgacggcgag cagtataaaa gcctggctgt actcgatacc
    34561 gtctttacgg cgttgttaca aaagccgcat ggtcgcgata ctacgctggt ggcgcttggc
    34621 ggcggcgtag tgggcgatct gaccggcttc gcggcggcga gttatcagcg cggtgttcgt
    34681 ttcattcaag tcccgacgac gttactgtcg caggtcgatt cctccgttgg cggcaaaact
    34741 gcggtcaacc atcccctcgg taaaaacatg attggcgcgt tctaccagcc tgcttcagtg
    34801 gtggtggatc tcgactgtct gaaaacgctt cccccgcgtg agttagcgtc ggggctggca
    34861 gaagtcatca aatacggcat tattcttgac ggtgcgtttt ttaactggct ggaagagaat
    34921 ctggatgcgt tgttgcgtct ggacggtccg gcaatggcgt actgtattcg ccgttgttgt
    34981 gaactgaagg cagaagttgt cgccgccgac gagcgcgaaa ccgggttacg tgctttactg
    35041 aatctgggac acacctttgg tcatgccatt gaagctgaaa tggggtatgg caattggtta
    35101 catggtgaag cggtcgctgc gggtatggtg atggcggcgc ggacgtcgga acgtctcggg
    35161 cagtttagtt ctgccgaaac gcagcgtatt ataaccctgc tcaagcgggc tgggttaccg
    35221 gtcaatgggc cgcgcgaaat gtccgcgcag gcgtatttac cgcatatgct gcgtgacaag
    35281 aaagtccttg cgggagagat acgcttaatt cttccgttgg caattggtaa gagtgaagtt
    35341 cgcagcggcg tttcgcacga gcttgttctt aacgccattg ccgatagtca atcagcgtaa
    35401 caacaagaaa ggtcaggccg cttatcaagc ggtctattag cttcaggtta attgcaatgt
    35461 ggtaagcatt aaccttttag tggggtgtta aatggatgaa ttcaaaccag aagacgagct
    35521 gaaacccgat cccagcgatc gtcgtactgg tcgttctcgt caatcttctg aacgttctga
    35581 gcgtactgaa cgtggcgaac cgcagatcaa ttttgatgat attgaacttg atgacactga
    35641 cgatcgccgt ccgactcgtg cgcaaaaaga gcgcaatgag gaaccggaaa tcgaagaaga
    35701 aattgacgaa tccgaagatg aaaccgtgga tgaagagcgc gtagagcgtc gtccgcgtaa
    35761 gcgcaaaaaa gcagccagta aacccgcttc tcgtcagtat atgatgatgg gcgtcggcat
    35821 tctggttcta ctgctgttga tcatcggtat cggttctgcg ctaaaagccc cctcgacctc
    35881 ttccagcgat caaaccgcgt ctggcgagaa gagtattgat cttgcaggca atgcgaccga
    35941 tcaggcgaat ggggtgcagc cagcgccggg aaccacgtct gcggaaaata ctcagcagga
    36001 tgtttctctg ccaccgatct cttctacgcc gactcaaggg caaaccccgg cggcaacgga
    36061 tggtcaacaa cgtgttgaag tgcagggtga cctgaacaat gcgctgaccc agccacaaaa
    36121 tcagcaacag ttgaacaatg tggcggtcaa ttctacattg ccgactgaac cagcaactgt
    36181 cgcgccagta cgcaatggca atgcatcgcg tgacacggcg aaaacgcaaa ctgctgaacg
    36241 tccggccact acgcgtccag ctcgtcagca ggcggtgatt gaaccgaaaa aaccgcaagc
    36301 aaccgtgaaa acggaaccga agccggtagc acagacgccg aagcgtactg aaccagctgc
    36361 tcctgtggcg agcacgaagg caccggctgc gacttctgcg ccagcaccaa aagagacggc
    36421 gactacggct ccagtacaga cggcatcccc ggcgcaaacc acggcaacac cagccgctgg
    36481 agggaagacc gcaggtaatg ttggttcgtt gaaatcggca ccgtccagcc attacactct
    36541 gcagctgagc agttcctcta actacgacaa cctgaacggt tgggcgaaga aagagaatct
    36601 gaaaaactac gttgtctatg aaacgacgcg taatggtcag ccgtggtatg tcctggtttc
    36661 tggcgtgtac gcttcgaaag aagaggcgaa aaaagcggta tctacattgc cagcagatgt
    36721 tcaggccaaa aacccgtggg cgaaaccgct gcgtcaggta caggccgatc tgaagtaatc
    36781 aaggttatct cccgcaatgg gttatcgttg cgggagttgc ctgaagcgct ggatgctgtc
    36841 ggagctttct ccacagccgg agaaggtgta attagttagt cagcatgaag aaaaatcgcg
    36901 cttttttgaa gtgggcaggg ggcaagtatc ccctgcttga tgatattaaa cggcatttgc
    36961 ccaagggcga atgtctggtt gagccttttg taggtgccgg gtcggtgttt ctcaacaccg
    37021 acttttctcg ttatatcctt gccgatatca atagcgacct gatcagtctc tataacattg
    37081 tgaagatgcg tactgatgag tacgtacagg ccgcacgcga gctgtttgtt cccgaaacaa
    37141 attgcgccga ggtctactat cagttccgcg aagagttcaa caaaagccag gatccgttcc
    37201 gtcgggcggt actgttttta tatttgaacc gctacggtta caacggcctg tgtcgttaca
    37261 atctgcgcgg tgagtttaac gtgccgtttg gccgctacaa aaaaccctat ttcccggaag
    37321 cagagttgta tcacttcgct gaaaaagcgc agaatgcctt tttctattgt gagtcttacg
    37381 ccgatagcat ggcgcgcgca gatgatgcat ccgtcgtcta ttgcgatccg ccttatgcac
    37441 cgctgtctgc gaccgccaac tttacggcgt atcacacaaa cagttttacg cttgaacaac
    37501 aagcgcatct ggcggagatc gccgaaggtc tggttgagcg ccatattcca gtgctgatct
    37561 ccaatcacga tacgatgtta acgcgtgagt ggtatcagcg cgcaaaattg catgtcgtca
    37621 aagttcgacg cagtataagc agcaacggcg gcacacgtaa aaaggtggac gaactgctgg
    37681 ctttgtacaa accaggagtc gtttcacccg cgaaaaaata attctcaagg agaagcggat
    37741 gaaacagtat ttgattgccc cctcaattct gtcggctgat tttgcccgcc tgggtgaaga
    37801 taccgcaaaa gccctggcag ctggcgctga tgtcgtgcat tttgacgtca tggataacca
    37861 ctatgttccc aatctgacga ttgggccaat ggtgctgaaa tccttgcgta actacggcat
    37921 taccgcccct atcgacgtac acctgatggt gaaacctgtc gatcgcattg tgcctgattt
    37981 cgctgccgct ggtgccagca tcattacctt tcatccagaa gcctccgagc atgttgaccg
    38041 cacgctgcaa ctgattaaag aaaatggctg taaagcggga ctggtattta acccggcgac
    38101 accgctgagt tatctggatt acgtgatgga taagctggat gtgatcctgc tgatgtccgt
    38161 caatcctggt ttcggcggtc agtcgttcat tcctcaaaca ctggataaac tgcgcgaagt
    38221 acgtcgtcgt atcgacgagt ctggctttga cattcgtctg gaagtagacg gtggcgtgaa
    38281 ggtgaacaac attggcgaaa tcgctgcggc gggcgcggat atgttcgtcg ccggttcggc
    38341 aatcttcgac cagccagact acaaaaaagt cattgatgaa atgcgcagtg aactggcaaa
    38401 gttaagtcat gaataagttt gaagatattc gcggcgtcgc ttttgatctt gatggtacgc
    38461 tggtcgacag tgctcccggt cttgctgctg cggtagatat ggcgctgtat gcgctggagt
    38521 tgcccgtcgc aggtgaagaa cgcgttatta cctggattgg taacggcgca gatgttctga
    38581 tggagcgcgc attgacctgg gcgcgtcagg aacgtgcgac tcagcgtaaa acaatgggta
    38641 aaccgcccgt tgatgacgac attccggcag aagaacaggt acgtattctg cgtaaactgt
    38701 tcgatcgcta ctatggcgag gttgccgaag aggggacgtt tttgttcccg cacgttgccg
    38761 atacgttggg cgcgttgcag gctaaaggcc tgccgctagg cctggtcacc aacaaaccga
    38821 cgccgttcgt cgcgccgctg ctcgaagcct tagatattgc caaatacttt agcgtggtta
    38881 tcggcggcga tgatgtgcaa aacaaaaaac cgcatccgga cccgctgtta ctggtggctg
    38941 agcggatggg aattgcccca caacagatgc tgtttgtcgg cgactcacgc aatgatattc
    39001 aggcggcaaa agcggcaggt tgcccatcag ttggcttaac ctacggatat aactacggcg
    39061 aggctatcga tctcagccag cctgatgtaa tttatcagtc tataaatgac cttctgcccg
    39121 cattagggct tccgcatagc gaaaatcagg aatcgaaaaa tgactaagcc catcgttttt
    39181 agtggcgcac agccctcagg tgaattgacc attggtaact acatgggtgc gctgcgtcag
    39241 tgggtaaaca tgcaggatga ctaccattgc atttactgta tcgttgacca acacgcgatc
    39301 accgtgcgcc aggatgcaca gaagctgcgt aaagcgacgt tggatacgct ggccttgtat
    39361 ctggcttgtg gtatcgatcc tgagaaaagc accatttttg ttcagtccca cgtaccagaa
    39421 catgcgcagt taggctgggc actgaactgc tatacctact tcggcgaact gagccgcatg
    39481 acgcagttta aagataaatc tgcgcgttat gccgagaaca tcaacgctgg tctgtttgac
    39541 tatccggtgc tgatggctgc ggacatcctg ctgtatcaaa ctaatctggt accggtgggt
    39601 gaagaccaga aacagcacct ggaactgagt cgcgatatcg cccagcgttt caacgcgctg
    39661 tatggcgaga tctttaaggt gccggagccg tttattccga aatctggcgc gcgcgtaatg
    39721 tcgctgctgg agccgaccaa gaagatgtcc aagtctgacg ataaccgcaa taacgttatc
    39781 ggcctgctgg aagatccgaa atcggtagtg aagaaaatca aacgcgcggt cactgactcc
    39841 gacgagccgc cggtagttcg ctacgatgtg cagaacaaag cgggcgtttc caacctgctg
    39901 gatatccttt ctgcggtaac gggccagagc attccagaac tggaaaaaca gttcgaaggc
    39961 aagatgtatg gtcatctgaa aggtgaagtg gctgatgccg tttccggtat gctgactgaa
    40021 ttgcaggaac gctatcaccg tttccgcaac gatgaagcct tcctgcaaca ggtgatgaaa
    40081 gacggcgcag aaaaagccag tgtacacgct tcccgtacgc tgaaagcggt gtacgaagcg
    40141 attggttttg tggcgaagcc gtaatatcaa aacgctcgga cggtcccctc gcccctttgg
    40201 ggagagggtt agggtgaggg gagcaggcaa gcgccagtgg gaattaccct ttgctcgcat
    40261 tgtcgaataa atcacttcgt catcactctg aaaccgggga aacccggttt ttttatcctc
    40321 atttgcaata atccgaaaaa atgtgaagcg cctcgccgtt tccacatctc acagttgcca
    40381 cttatcttca ttttaatgaa gatataaaca ttcattttat tgaaaatata ttatgcgtcg
    40441 aacgtttatc aaaaaagaag gcgtcgttat cacgacgctg gcccgttatt tgttgggtga
    40501 aaaatgcggt aataggttga aaaccataga tgagctggca actgaatgcc gttcatccgt
    40561 tggcctgacg caggccgcgt tgaaaacgct ggaatcaagc ggagcgatac ggattgaacg
    40621 ccgtgggcgc aatggcagtt atctggtcga gatggataac aaagcattgc tgagtcatgt
    40681 ggatatcaac aacgtggtat gtgcgatgcc tttgccctat acccgcttgt acgaaggttt
    40741 agcgagcgga ttgaaagccc agtttgatgg cattcctttt tactatgcgc atatgcgcgg
    40801 cgcggatatt cgcgtggagt gtttacttaa cggcgtgtat gacatggcgg tggtttcccg
    40861 actggcggcg gaaagttatc tcacgcaaaa agggttttgc ctcgcgctgg agttggggcc
    40921 gcacacctac gttggcgagc accagttgat ttgccgtaaa ggcgagtccg caaacgtgaa
    40981 gcgcgtgggg ctggataacc gttcggcgga tcagaaaatc atgaccgatg tcttttttgg
    41041 cgatagtgat gtggaacgtg tcgatctctc ttatcacgag agtttacaac gcattgttaa
    41101 aggcgatgtc gatgcggtga tctggaacgt ggtggcggaa aacgaactga ccatgctggg
    41161 attagaggcg acgccgctca cagacgatcc gcgattttta caggccaccg aagccgtggt
    41221 tctgacgcga gtcgatgatt acccaatgca acaactgctg cgtgccgttg tagataaaca
    41281 cgccctgctc gcccatcaac aacgggtagt gagtggggaa caggaaccga gttattaaac
    41341 gaaaggcatc tgatatggaa accagactca acctgctttg cgaggcaggc gttattgata
    41401 aggacatctg caaaggcatg atgcaggtcg tcaacgtact ggaaacagag tgccatctgc
    41461 cggtgcgcag tgagcaagga acgatggcga tgacacatat ggcgagtgca ctgatgcgca
    41521 gtcgccgtgg tgaagaaata gagccgctgg ataacgaatt gctggcagag ctggcgcaat
    41581 ccagccactg gcaagccgtt gtgcaattgc atcaggtgtt gttgaaggaa ttcgcactgg
    41641 aagttaaccc gtgtgaagaa ggctatttgc tggccaacct ttatggatta tggatggctg
    41701 ctaacgaaga ggtttgaagc tcccgacaaa tccacgtcgc acaacctaaa tattcaaaaa
    41761 aatgaatatt taattcaagg aacagaaacg aggcaacaag atgtttgtag aagcattgaa
    41821 acgccagaac ccggcgctga tttccgccgc actaagtttg tggcagcagg gcaagatcgc
    41881 cccggacagc tgggtgatcg acgtggatca ggtactggaa aacggtaagc gactgattga
    41941 gacggcgcgg ctttacggca ttgaactgta tctgatgacc aagcagtttg gccgtaaccc
    42001 atggctggcg gaaaaattgc tggcattagg ttacagcggc attgtggcgg tggattacaa
    42061 agaggcgcga gtcatgcgcc gcgctggttt gtcggtggcg catcaggggc atctggtaca
    42121 aatcccttgt catcaggttg ctgacgccgt tgaacagggc accgacgtca tcaccgtgtt
    42181 tactctcgac aaagcgcggg aagtttctgc ggcagcggtg aaggccggac gtgtgcagtt
    42241 tgtgctgctc aaagtttata gcgacgatga ttttctttat ccggggcagg agagcggttt
    42301 tgtacagcat tcgctgcacg aggtggttgc tgaaattaag aaactgccgg gtctgcattt
    42361 agccggactt acccatttcc cttgcctgct ttgggatgag gctgccggga aagttttgcc
    42421 gacaccgaat cttcacacgc tggtacaagc acgggatcaa ctggcgaaat ctggtattgc
    42481 gattgagcaa ctgaacgcgc cttctgcgac cagctgcact tcgctgccga tgctggcgga
    42541 atacggtgtg actcatgccg aacccggtca tgcgctgacg ggcactattc cggcaaacca
    42601 gcagggcgat cagcctgaat atatagcgat gctttggtta agtgaaatct cccatcattt
    42661 ccgtggcgac agctactgct acggcggcgg ttactaccgt cgtggtcatg cgcaacatgc
    42721 gctggtgttt acgccagaaa atcaaaagat tactgaaacc aatctcaaaa ctgtggatga
    42781 cagcagtatc gactacaccc tgccgctggc aggcgagttt ccggtaagca gtgcagtagt
    42841 gctctgtttt cgcacgcaga tttttgtcac tcgtagcgat gtggtgctgg tgtccggtat
    42901 tcaccgtggc gaaccgaaaa tcgtcggtcg ttatgacagt cttggaaact ctctgggggc
    42961 gtaatggcgc gatttgtggt gttagtgatt gatagctttg gcgtaggggc aatgaaagat
    43021 gtcacgctgg tgcgcccgca agatgccggg gcgaatacct gtggtcacat cctgagccag
    43081 ttgccgcatt tgcagctacc agcgctggag aagctggggc taatcaacgc attgggttat
    43141 gcaccaggtg atatgcaacc gtcagattcc gcaacctggg gggtggcaga gctgcaacat
    43201 gaaggtggcg atacctttat ggggcatcag gaaattttag gcacgcgccc gttaccgccg
    43261 ctgcggatgc cctttcgcga tgtgattggc cgtgttgagc aggcattagt ttccgctggc
    43321 tggcaggtgg agcgccgtgg cgatgatctg caatttctgt gggtcaatca ggcggttgcg
    43381 attggcgata atctcgaggc ggatttaggc caggtctata acattaccgc caatctctct
    43441 gtgatctctt ttgacgacgc gatcaaaatt ggtcgtatcg tgcgtgagca ggtacaggtc
    43501 ggtcgggtca ttacatttgg cggcttgtta accgacagtc agcgtattct cgatgccgca
    43561 gaaagcaaag aagggcgctt tattggtatt aatgcgccgc gttctggcgc ttatgacaac
    43621 ggcttccagg tcgtgcatat gggctacggt gttgatgaaa aagtgcaggt gccgcaaaaa
    43681 ctgtatgagg cgggcgtgcc aaccgtgctg gtaggtaagg tggcagatat cgtcagcaat
    43741 ccttatggcg tgagctggca aaacctggtg gatagccagc ggattatgga tatcaccctc
    43801 gacgaattta acacgcatcc gacggcgttt atttgcatca atatccagga aaccgacctc
    43861 gctggtcatg cagaagacgt cgcacgttat gccgaacgtt tgcaggtcgt tgaccgtaac
    43921 cttgcccggc ttgttgaggc gatgcagcca gatgattgcc tggtcgtgat ggcggatcac
    43981 ggtaacgatc cgaccattgg tcacagccac catacccgtg aagtggtgcc agtgctggtt
    44041 tatcagcaag ggctggtcca cacgcaactc ggtgtacgca ccacgctttc tgatgtgggg
    44101 gctaccgtgt gtgaattttt ccgtgcgcca ccgccacaaa atggtcgctc ttttctttcc
    44161 tccctccggt ttgcaggaga caccctatga gtattgatcc gacgggttac accctggccc
    44221 atgagcatct gcatattgat ctctccggct ttaaaaacaa cgtggactgc cgccttgatc
    44281 agtatgcgtt catttgccag gagatgaacg acctgatgac ccggggcgtg cgtaatgtga
    44341 ttgagatgac caaccgttac atggggcgca atgcgcaatt tatgctcgat gtaatgcgcg
    44401 agacggggat caacgtggtg gcctgcaccg gttattacca ggacgctttt ttcccggagc
    44461 atgtggcgac ccgcagcgtg caggaactgg cgcaggagat ggtcgatgaa attgaacagg
    44521 gtatcgatgg caccgacctg aaagccggga ttatcgcgga gatcggctct agcgaaggga
    44581 agattacgcc gctggaagag aaggtgttta ttgccgctgc gctggcgcat aaccagaccg
    44641 gacgcccgat ctccacgcat acttcgttca gcaccatggg gctggaacaa ctggctctgt
    44701 tgcaagccca cggagttgat ctttcgcgcg tcaccgttgg tcactgcgat ctgaaagaca
    44761 atctcgacaa cattttgaag atgatcgatc tcggcgcgta cgtgcagttc gacaccattg
    44821 gtaagaacag ttactacccg gacgaaaagc gcattgcgat gctccacgcg ctacgtgacc
    44881 gtgggttgct gaaccgcgtc atgctgtcga tggatattac gcgccgctcc catttaaaag
    44941 ccaatggtgg ttatggctat gactatttat tgaccacctt tattccgcaa ttgcgccagt
    45001 caggattcag tcaggccgat gtggatgtga tgttacgtga aaatccctct caatttttcc
    45061 aataaggaca gactcatgaa aaagattggc gttgcaggct tacagcgtga gcagattaaa
    45121 aaaaccattg aagcgacggc tcctggcagt tttgaagttt tcattcacaa cgacatggaa
    45181 gcggcaatga aagtgaaatc cgggcaactg gattactaca tcggtgcgtg taataccggt
    45241 gcgggcgcgg cattgtcgat tgccatcgcg gtgattggct ataacaaaag ttgcaccatt
    45301 gccaaaccag gcattaaagc gaaagacgag catatcgcca aaatgatcgc tgaagggaaa
    45361 gtggcgtttg gcctttccgt tgagcacgtc gaacacgcga ttccgatgct gattaaccat
    45421 ctgaaataaa aggcacgact atggatctgt atattcagat tatcgtggtg gcgtgcctga
    45481 cgggtatgac atcgcttctg gcgcatcgct cggcggctgt ttttcatgac ggcatccgcc
    45541 cgatcctgcc gcaactgatt gaaggctata tgaatcgtcg cgaggcgggg agtatcgctt
    45601 ttggtctgag cattggtttt gtggcctcgg tggggatctc ttttaccctg aaaaccgggc
    45661 tgctcaacgc atggttactc tttcttccta ccgatatcct cggcgtactg gcgataaaca
    45721 gcctgatggc gtttggtctt ggcgctatct ggggcgtgtt gatccttact tgcctgttgc
    45781 cagtaaacca gctgctgacc gcgctgccgg tggatgtatt aggtagcctc ggggaattaa
    45841 gctcgccggt ggtttctgct tttgcactgt tcccgctggt ggcgattttc taccagtttg
    45901 gctggaagca aagtctggtc gccgccgtgg ttgttctgat gacccgtgtg gtagtcgtgc
    45961 gctatttccc acatcttaac cctgaatcca tcgaaatctt tattggcatg gtgatgctgc
    46021 tggggatcgc gataactcac gacctgcgtc atcgtgatga aaatgacatc gatgccagcg
    46081 ggctttcggt gtttgaagag cgcacgtcgc ggattatcaa aaacttacct tatatcgcca
    46141 tcgtgggagc attgattgcc gccgttgcca gtatgaagat ttttgctggt agcgaagttt
    46201 cgatcttcac actggagaaa gcgtattccg caggcgtgac gccggaacaa tcgcaaacgc
    46261 tgattaatca ggcggctctg gcagaattta tgcgcggact gggttttgtg ccgttgattg
    46321 ccaccaccgc gttagccact ggcgtgtatg cagttgcggg ctttaccttt gtttatgcgg
    46381 tgggctatct ctcgccaaat ccgatggttg cggcggtatt aggcgcagtg gttatttcgg
    46441 cggaagtctt gctgcttcgt tcgatcggca aatggctggg acgctacccg tcggtgcgta
    46501 atgcgtcgga taacatccgt aacgccatga atatgctgat ggaagtggcg ctgctggtcg
    46561 gttcgatttt cgcagcaatt aagatggcgg gttataccgg attctctatc gcggttgcca
    46621 tttacttcct caacgaatcc ctgggccgtc cggtacagaa aatggcggca ccggtcgtgg
    46681 cagtaatgat caccggtatt ctgctgaatg ttctttactg gcttggcctg ttcgttccgg
    46741 cttaaggaag gcacctatga agacgtttcc tctgcaaagc ctgacgatta ttgaggcgca
    46801 gcaaaagcag tttgcgctgg tggatagcat ttgtcgccat ttccccggca gcgagtttct
    46861 tactggcggt gatttagggt taactccagg tctgaatcaa ccgcgtatta cccaacgggt
    46921 ggagcaggtg ctggctgatg catttcacgc acaggctgcg gcgctggtgc agggcgcggg
    46981 gactggcgcg attcgcgccg ggctggcggc tttgctcaaa ccggggcagc gtcttctggt
    47041 gcatgacgcg cctgtttacc cgacgacacg ggttattatt gagcagatgg ggctgacgct
    47101 tattactgtt gatttcaatg acctgtcggc actgaagcag gtcgtcgacg agcaacaacc
    47161 ggatgcggcg ctggtgcagc atacgcgcca gcagccgcag gacagctacg tgctggcaga
    47221 tgtgctggca acgttgcgcg cggcaggtgt tccagcgtta accgatgaca actatgcggt
    47281 gatgaaggtg gcgcgaatcg gctgtgaatg cggcgcgaat gtctcgacat tttcctgctt
    47341 caagctattt gggccagagg gtgttggtgc agtggtcggc gatgctgatg ttatcaaccg
    47401 tattcgcgcc acgctttact ccggcggtag ccagatccag ggcgcacagg cgctggaagt
    47461 attgcgtggt ctggtgtttg cgccagtgat gcacgcggtg caggcagggg tatctgaacg
    47521 gttgctggct ttgcttaacg gtggtgcggt gccggaagtg aaaagcgcgg tgattgctaa
    47581 tgcgcagtcg aaggtgttga ttgtcgagtt tcatcagccg attgccgcca gagtgctgga
    47641 agaggcgcaa aagcgcggtg ccttgcctta cccggtgggt gcagagtcga aatatgaaat
    47701 cccgccgctc ttttatcgcc tttccggaac gtttcgccag gcgaatccac aatcagaaca
    47761 ttgtgcgatt cgcattaacc cgaatcgcag cggtgaagag acggtgctgc ggattttgcg
    47821 tgagagtatt gccagtattt aatgctggat agcaccgtca agctaaattc cgtactgaac
    47881 ggtcccctcg cccctttggg gagagggtta gggtgagggg aacgtacggg atgtaaaatt
    47941 caacgcgcca gcatcgccgg atgcgatgct ggcgcatctt atccggccta cggttaatgt
    48001 cttttattat caatcgttaa ggtgatgcga ttggcgcggc agaagagttc ggagacgtgc
    48061 accggtttgt cattctgatc aaaggcgatt ttgctgatgc gaaacaacgg ttcgcccagt
    48121 tcgcatttca gccattttgc ctgcgggcgg gtggcggcaa agatatcgat ggtctttttg
    48181 tcgctgacca ctcgcgtgtc gaaacgctcc tgaaataact gataagtgga gcttccttca
    48241 acgtaaatct catcaaagtc aggataacgc gacagtggga tccaggaact atcaataaac
    48301 agcggctctt tatcgagata catcacccgg caaagatgga acacttcgct gttgccaggg
    48361 atgttcagct tttcgcaaaa cggcgcggcg ctgactcgtt cctgctcgat cactttctct
    48421 ttcgtcgcct tgccttgtga gacgccaaaa tcggtaaaac cactgacggt cagcagggcg
    48481 ttttcgacct tctggctttg tacaaaggtg cctttgccct gccagcggat cagtacgccg
    48541 tctgccacta agtcgctgat agctttgcga atagtaatgc ggctgacgtt atattgcgta
    48601 caaagctcgt tttcggtagg gatctgttgc ccggcctggt aaaccccctg cgcgatatca
    48661 tccagcaacc gctggcggac ggtagcgtag aggagttgat gagagtagcg gtccgtagct
    48721 gacataatcg accctgaaat aaagaatgaa ttactcggat tatagggtcg ttttccggca
    48781 agtaacagca cgttggcgtg aagatgctca cgccaacgtt atacctacca ggcaccgtgg
    48841 tactgaatgg ttttcgccgc gcacgccgtt ccctgcgcca tcgcctgcgg taatgtcatc
    48901 cccgcagacc agccgcaaag gaatccggca atgaacgaat ctccggcacc catggtgtcg
    48961 ataaccgtca ccggttcagg agcctgacgc cagaactgcg cgccatccca ggcaatgctg
    49021 ccgttttcac ccagcgtgac aatcaccacg cctgcgccac gggcaacaat cgctttcatc
    49081 ttcagacgca gcgcttcgtc ttcttgcggt gcggaggcaa aggcaaaatc gagatgcggc
    49141 accagtgtct gccagagcga gctgtcccac ttgtcggaga agtcgaaagc ggtaagtttg
    49201 cccgcagcgt gcagctgtgg gaatgcgtct tccgcatgtc cccagattgc cgcgtgcaca
    49261 atgtcatact gcgccagcca ggcgtaatcc tcttcactca gggcaaagtc ggccatcacg
    49321 ccttcggtgt agtcgccaaa aacgcgatca ttgtcgtgca gttccacctg agtttgtgcg
    49381 gtaacgccgt gtttcgtatg gacatggctg atatcgacgc ccatgcgggc gagatcctgc
    49441 ttcagctttg tgccgtagtc atcgtcaccc acccaggtaa tgcatcccgg ctgtatgccg
    49501 tagcgagtgc agtacaccgc cacattgacc gcattaccgc cagaaaacgc tttattcagt
    49561 tgcgggtaga tatcgacgca gttatcgccg attgtcgcca gggttttcat ctctcatcct
    49621 ccggcagcag cgcgcgaaag cgttccagcg cctggcgggc atagagtctg ggctcgttca
    49681 tatacatcgt caccagctcc accgtacagt aaccctcata gccccggtcg ataatatcgc
    49741 gcatcagttc cctcagcggc attttgcctt ctccaggaat gtaatgcgtg tcgctggccc
    49801 cgtcgctgtc gacaatatgc agatgacgta atttatcgcc cagtttgtcg aaataactca
    49861 tcaccggttc cgcctggaca tacggcgcgc aaatgtcgac catgctgaac aggcgcggcg
    49921 aaggcaccag cgccagcgca tgaagcacat cattagcatt acacacgacg ttcgattcat
    49981 acggcgttaa cggttcgagg attagatcca tgccgatgtt ttccgcgtac tcacacagtt
    50041 cgcttaggtt ctctgccagc cgtccccaga taacattagg tggcgtgaga tagcccgcgt
    50101 gggccgcgga aatcagcgta taacccgcgt tcatctcttt tgccatatcc atcgccagct
    50161 tgatcatgtc gaggctttcg cgacgcatat gttcatcgcc cagcatcatg ttatacggat
    50221 agccgttggt ttctggcgta tagccgataa tcggcatctg atacgtctgc gccagcgcct
    50281 tgatttgttt gatgccgccc gcttttaagt ccggcgcgaa cgcgtgcggg cgaccgcccc
    50341 aaatttcgat gccgtcgtaa cccagctcgc ttgcatcacg aaatgcgtgt tcaataggca
    50401 gccgctggtg gccgcaggta aacatacctg ttttcatcgg gtaactcctt gcgtaaattc
    50461 ggcccgttgc cgggccggga gagggattaa tattccacca gaccaccgta atagcggcgt
    50521 tcatccgggt tgtgatcttt gtaaatagac aggtagtagc agagccactc cattggcacg
    50581 aacatcagga acggtgccag ccacgggtgc agcccttgcg aaatttcggc gtaatcgatg
    50641 acgatcacgt tgtcagtacg ctgtttaaca aagttaatgg cgcgttcggt ggtgtggcga
    50701 ctttcatcat tgccgagcag gaacaggaac ggaacgcccg gttcgacaat ctccagcggg
    50761 ccatggcgga actctccgct ctcaatcacg cagccgtgcg tccaggtaaa ttccatcagc
    50821 gttacaatgc cttctttgta acccagcgga cgcagcggac ccgcagcaac ggtataaatc
    50881 atcggccact ggctggccag ttcaccaagc tggcggcctt tttcttccca ggtgcgtacc
    50941 agttgaccga gcgcattcgg caactgcttg agatcgtttt tgatcttgcc gatttccgcg
    51001 ttcggcgcga ggcgggtgat catctccagc accacgctgt agcagagcag caggtgaatt
    51061 tcccagatac agtcggcctg ataatcaatg ctaaattccg ccgccgaggt aatcgggctg
    51121 tccgcgcgtt tggtgaacgc cgcagtgagt gcgccgcagg cccggcccag ctccagcgct
    51181 ttgattacct cttccgtttt accgtagtca gaaacgccaa tcaccgcgca acgatcgtcg
    51241 aggcgatacg gggtgttatc gcagaactcc cagccggaaa tggcgtagac ctgaagatcg
    51301 gaaaagcgat ccgccagatg tttcgccgtt tgcgccgcgt tgagtggcga tccgcaggca
    51361 acgaaataaa tacgatcaat gtcgcgcttc accatctctt ccacgatggt gtgcaccagc
    51421 ggaacgtcat ggctgagaac tttttccact tcctgaacca tattttcggt gaccagaaag
    51481 tccacggtgc ttttatcaat atccaacatt ttattttctc cagataacat tacgacaaag
    51541 cgttgagctg gcggctacgc ttcgcccaga aggcgtaagc aggcagacca gtagcaataa
    51601 cgatgacggc gcagataagg ccgggaatcg gtgcccagac aaaggttgag gcgaccagaa
    51661 tgaggcttga cgcaatggcg agggtggtca tcagcccgaa agccggagta cgccacagcg
    51721 gtttgtaatc gtcgcgttta cgacaccaga tgatggagcc gaaggtgagg gtatttttga
    51781 aacacatcac cagggtgaaa taacccagca ggctggtgag atcggaaacg aagatgaaga
    51841 agatccccag cgccccttgc aggatgatgg agacatccgg cgtgttgtat ttcggatgca
    51901 catggccgaa gcatttaaag aacaggttgt ctttcgccat cgcgtattcc agccgcggct
    51961 ggtacatcac gcagctggaa agcgaaccaa gaatgacgat catcgccgtg atggcaacaa
    52021 agatcccagc ggtgctgccg agtgcgggga tccaggtcag ggcgtcggaa atcggcgttt
    52081 cagaattggc gagtttgtcg aagggcatca ggccggaaat caccagcgcc agcagggtgt
    52141 agagcaccag aaccagcaga caggaaccaa tcagcgctcg tggcatggtt tttccggggt
    52201 ttttaatttc gccggtcata taacagatag aggccatgcc ggtatacgac caactggtgg
    52261 cagagatccc cgccagcagc gccataaagc tgcccgttgc gccaatcgca gtggtggtag
    52321 gggcggcaaa attctccgct ttaaaccaga agatcccaag gccaatgacg atagtgaacg
    52381 ggataatttt ggcgatggta attagcgtct gaaacgctgc gccgccttca acggagcgca
    52441 ggtgtagcaa cataaaggcg ataattaatc cggcggcgat aaatttaccg agcaacggat
    52501 cgataggcgt taaaaagcca agattgctga caatcgccag cgccataatc gacaatgacg
    52561 gcgcatcgtt ggcccagaag ctggcccagc cggagaggaa agccagcggt cggcttccgg
    52621 catttttcag ataaacataa tctgcgccat tttccggata agcggtggat agttccgcat
    52681 agacgcacat ttgcgggatc acaattaacc cgccaatgac aaacgcgagc accgtaagcc
    52741 acggcgtgcc cgctgctttt gccacttcac ccacagatac aaaaataccg gagccgacgg
    52801 ttgtcccgac ggcgattgca agaacggccc aaaatccgag cttgcgttgg agttcctggc
    52861 ttcccatagc gatacctttt tcttgtccaa tacgttgtag gggagagttt gcagtcattt
    52921 aaaaggtaac atgacaatgc atgatgaata taacaacata tgatgttatg taatgtggag
    52981 gaagatcaca aaatttcgca caggatcgcg ctgtggctaa tggatgtagt tatcaaattg
    53041 aatttaaagt gaaaatattt ttacgggcgg gggcaagaag gacatagaaa caaatacgcc
    53101 ctcggaaaat ccagagggcg tcgggcaatt aaaccggtgt tagccgattt ctgtcagaga
    53161 cttactgtgc agtaggaccg caaccgccaa tgattttaga aatagaaatt gccgggtgca
    53221 gcaggtagtc gtaagagcag ttcttatcac ggttttcaat atgaccagta catgcagtta
    53281 acgtagccag tactgcacct accagtgcaa ctttaata
//
LOCUS       DAZFRJ010000010        48282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000010 DAZFRJ010000000
VERSION     DAZFRJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.010"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            165..1382
                     /gene="alaA"
                     /locus_tag="ACHKUN_000535"
     CDS             165..1382
                     /gene="alaA"
                     /locus_tag="ACHKUN_000535"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO6658849.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1466..2065
                     /gene="yfbR"
                     /locus_tag="ACHKUN_000536"
     CDS             1466..2065
                     /gene="yfbR"
                     /locus_tag="ACHKUN_000536"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6658850.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2124..3956)
                     /locus_tag="ACHKUN_000537"
     CDS             complement(2124..3956)
                     /locus_tag="ACHKUN_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6658851.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4043..4693)
                     /gene="hxpA"
                     /locus_tag="ACHKUN_000538"
     CDS             complement(4043..4693)
                     /gene="hxpA"
                     /locus_tag="ACHKUN_000538"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO6658852.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4704..5198)
                     /locus_tag="ACHKUN_000539"
     CDS             complement(4704..5198)
                     /locus_tag="ACHKUN_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO6658853.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5281..5736)
                     /gene="yfbV"
                     /locus_tag="ACHKUN_000540"
     CDS             complement(5281..5736)
                     /gene="yfbV"
                     /locus_tag="ACHKUN_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO6658854.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6074..7276
                     /gene="ackA"
                     /locus_tag="ACHKUN_000541"
     CDS             6074..7276
                     /gene="ackA"
                     /locus_tag="ACHKUN_000541"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO6658855.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7351..9495
                     /gene="pta"
                     /locus_tag="ACHKUN_000542"
     CDS             7351..9495
                     /gene="pta"
                     /locus_tag="ACHKUN_000542"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6658856.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9685..11205
                     /gene="yfcC"
                     /locus_tag="ACHKUN_000543"
     CDS             9685..11205
                     /gene="yfcC"
                     /locus_tag="ACHKUN_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO6658857.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11238..11780)
                     /gene="yfcD"
                     /locus_tag="ACHKUN_000544"
     CDS             complement(11238..11780)
                     /gene="yfcD"
                     /locus_tag="ACHKUN_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO6658858.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11838..12389)
                     /gene="yfcE"
                     /locus_tag="ACHKUN_000545"
     CDS             complement(11838..12389)
                     /gene="yfcE"
                     /locus_tag="ACHKUN_000545"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO6658859.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12445..13089)
                     /gene="yfcF"
                     /locus_tag="ACHKUN_000546"
     CDS             complement(12445..13089)
                     /gene="yfcF"
                     /locus_tag="ACHKUN_000546"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO6658860.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13225..13872
                     /gene="yfcG"
                     /locus_tag="ACHKUN_000547"
     CDS             13225..13872
                     /gene="yfcG"
                     /locus_tag="ACHKUN_000547"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO6658861.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13929..14291
                     /gene="folX"
                     /locus_tag="ACHKUN_000548"
     CDS             13929..14291
                     /gene="folX"
                     /locus_tag="ACHKUN_000548"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO6658862.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14312..15205
                     /locus_tag="ACHKUN_000549"
     CDS             14312..15205
                     /locus_tag="ACHKUN_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO6658863.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15253..16143)
                     /gene="rpnB"
                     /locus_tag="ACHKUN_000550"
     CDS             complement(15253..16143)
                     /gene="rpnB"
                     /locus_tag="ACHKUN_000550"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO6658864.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="ACHKUN_000551"
     CDS             complement(16340..17113)
                     /gene="hisP"
                     /locus_tag="ACHKUN_000551"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO6658865.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17121..17837)
                     /locus_tag="ACHKUN_000552"
     CDS             complement(17121..17837)
                     /locus_tag="ACHKUN_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6658866.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="ACHKUN_000553"
     CDS             complement(17834..18520)
                     /gene="hisQ"
                     /locus_tag="ACHKUN_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO6658867.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="ACHKUN_000554"
     CDS             complement(18610..19392)
                     /gene="hisJ"
                     /locus_tag="ACHKUN_000554"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO6658868.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19613..20395)
                     /gene="argT"
                     /locus_tag="ACHKUN_000555"
     CDS             complement(19613..20395)
                     /gene="argT"
                     /locus_tag="ACHKUN_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO6658869.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="ACHKUN_000556"
     CDS             complement(20661..21230)
                     /gene="ubiX"
                     /locus_tag="ACHKUN_000556"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO6658870.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21325..22842)
                     /gene="purF"
                     /locus_tag="ACHKUN_000557"
     CDS             complement(21325..22842)
                     /gene="purF"
                     /locus_tag="ACHKUN_000557"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO6658871.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="ACHKUN_000558"
     CDS             complement(22879..23367)
                     /gene="cvpA"
                     /locus_tag="ACHKUN_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO6658872.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="ACHKUN_000559"
     CDS             complement(23626..24288)
                     /gene="dedD"
                     /locus_tag="ACHKUN_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO6658873.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24278..25546)
                     /gene="folC"
                     /locus_tag="ACHKUN_000560"
     CDS             complement(24278..25546)
                     /gene="folC"
                     /locus_tag="ACHKUN_000560"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO6658874.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25616..26530)
                     /gene="accD"
                     /locus_tag="ACHKUN_000561"
     CDS             complement(25616..26530)
                     /gene="accD"
                     /locus_tag="ACHKUN_000561"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO6658875.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26686..27345)
                     /locus_tag="ACHKUN_000562"
     CDS             complement(26686..27345)
                     /locus_tag="ACHKUN_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6658876.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27428..28240)
                     /gene="truA"
                     /locus_tag="ACHKUN_000563"
     CDS             complement(27428..28240)
                     /gene="truA"
                     /locus_tag="ACHKUN_000563"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO6658877.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28240..29253)
                     /locus_tag="ACHKUN_000564"
     CDS             complement(28240..29253)
                     /locus_tag="ACHKUN_000564"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6658878.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="ACHKUN_000565"
     CDS             complement(29319..30455)
                     /gene="pdxB"
                     /locus_tag="ACHKUN_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO6658879.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30554..31549
                     /gene="flk"
                     /locus_tag="ACHKUN_000566"
     CDS             30554..31549
                     /gene="flk"
                     /locus_tag="ACHKUN_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO6658880.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31546..32724)
                     /locus_tag="ACHKUN_000567"
     CDS             complement(31546..32724)
                     /locus_tag="ACHKUN_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6658881.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33008..34228)
                     /gene="fabB"
                     /locus_tag="ACHKUN_000568"
     CDS             complement(33008..34228)
                     /gene="fabB"
                     /locus_tag="ACHKUN_000568"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO6658882.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34387..36393
                     /gene="mnmC"
                     /locus_tag="ACHKUN_000569"
     CDS             34387..36393
                     /gene="mnmC"
                     /locus_tag="ACHKUN_000569"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO6658883.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36514..36792)
                     /locus_tag="ACHKUN_000570"
     CDS             complement(36514..36792)
                     /locus_tag="ACHKUN_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO6658884.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36826..37374)
                     /gene="epmC"
                     /locus_tag="ACHKUN_000571"
     CDS             complement(36826..37374)
                     /gene="epmC"
                     /locus_tag="ACHKUN_000571"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO6658885.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37374..38183)
                     /locus_tag="ACHKUN_000572"
     CDS             complement(37374..38183)
                     /locus_tag="ACHKUN_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6658886.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38183..39007)
                     /gene="mepA"
                     /locus_tag="ACHKUN_000573"
     CDS             complement(38183..39007)
                     /gene="mepA"
                     /locus_tag="ACHKUN_000573"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO6658887.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39011..40096)
                     /gene="aroC"
                     /locus_tag="ACHKUN_000574"
     CDS             complement(39011..40096)
                     /gene="aroC"
                     /locus_tag="ACHKUN_000574"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO6658888.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40131..41063)
                     /gene="prmB"
                     /locus_tag="ACHKUN_000575"
     CDS             complement(40131..41063)
                     /gene="prmB"
                     /locus_tag="ACHKUN_000575"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6658889.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41229..41780
                     /gene="smrB"
                     /locus_tag="ACHKUN_000576"
     CDS             41229..41780
                     /gene="smrB"
                     /locus_tag="ACHKUN_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO6658890.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41853..42704)
                     /locus_tag="ACHKUN_000577"
                     /pseudo
     CDS             complement(41853..42704)
                     /locus_tag="ACHKUN_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
     gene            complement(42706..43245)
                     /locus_tag="ACHKUN_000578"
     CDS             complement(42706..43245)
                     /locus_tag="ACHKUN_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6658891.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43242..43730)
                     /locus_tag="ACHKUN_000579"
     CDS             complement(43242..43730)
                     /locus_tag="ACHKUN_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6658892.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43727..44236)
                     /locus_tag="ACHKUN_000580"
     CDS             complement(43727..44236)
                     /locus_tag="ACHKUN_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6658893.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44252..45004)
                     /locus_tag="ACHKUN_000581"
     CDS             complement(44252..45004)
                     /locus_tag="ACHKUN_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6658894.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45024..47669)
                     /gene="yfcU"
                     /locus_tag="ACHKUN_000582"
     CDS             complement(45024..47669)
                     /gene="yfcU"
                     /locus_tag="ACHKUN_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO6658895.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDVGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47751..>48282)
                     /locus_tag="ACHKUN_000583"
     CDS             complement(47751..>48282)
                     /locus_tag="ACHKUN_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6658896.1"
                     /translation="AMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVPDDHKLEVD
                     MGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTD
                     TGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAP
                     DLGNFEANTTFQITYL"
BASE COUNT        11759 a        13104 c        12101 g        11318 t
ORIGIN      
        1 ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc ctctggcaaa
       61 atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc taagctgact
      121 tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa
      181 aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg aaagaagcaa
      241 aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac ccagccccgt
      301 tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc
      361 aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg cagcactacc
      421 aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat ggtgtatcgg
      481 agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg ttggttcctg
      541 caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt
      601 atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc gctaaaatta
      661 cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt
      721 ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc attatcttcg
      781 ccgatgaaat ttatgacaaa attctttacg acgacgctga gcatcactca attgcgccgc
      841 tggcacctga cctgctgacc attaccttta acggactgtc gaaaacgtac cgcgttgcag
      901 gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg
      961 aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca
     1021 ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc
     1081 tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga tattccgggc gtttcctgcg
     1141 tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc tttaacattc
     1201 acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag
     1261 ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg
     1321 atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat catcagctgt
     1381 aatcttaatt tcactgccgg agattgcatc cggcagcgtt atcccgccac aatgacctga
     1441 tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg
     1501 cctgaaactc attaaccgct ggccgctcat gcgcaacgtg cggacggaaa atgtgtccga
     1561 acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa atcggaaatt
     1621 tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga
     1681 agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa tcgctcagga
     1741 gtacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagct
     1801 gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag aaaaatcgct
     1861 ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg
     1921 aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag
     1981 ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt cgctcgatga
     2041 gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg
     2101 ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa
     2161 accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact gaagctgtaa
     2221 tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg
     2281 gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat cgttttggca
     2341 gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat aaatagccca
     2401 ataaccgccg acaagacaaa caaacacccc agcatcatat gtggcccgta accaccgcca
     2461 atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca
     2521 aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt ataggcgctt
     2581 tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga
     2641 ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa aatggcatgg
     2701 ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg
     2761 aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg
     2821 atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc
     2881 tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg caccgattta
     2941 ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg
     3001 tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc
     3061 cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca
     3121 gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg
     3181 acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc tatcattggc
     3241 gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga
     3301 tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc ccctttcagc
     3361 atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt
     3421 gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc
     3481 aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc aaaacttaac
     3541 ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa caccacggga
     3601 ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc
     3661 atcaacaaaa ccaacatttt gatttcactg ttgcccgcca ctttgaccag ccatgttccc
     3721 attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa ggcggcaatc
     3781 aggacaacgt taggatcaga aaagccggaa aatacttcgg ggaccgtcag cgttccgctt
     3841 aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca
     3901 aacaagacga tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact
     3961 tatccttgtt aattgagggg gatgacttga ttctgccata aaaaagcccc gacgagacgg
     4021 ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca
     4081 cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg
     4141 ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc acgccagcgg
     4201 gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc gcacctaaca
     4261 gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca aacacctctg
     4321 gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca
     4381 caatggccca cggaatacct gctttattca aatgattgag taaggcgatt gcccccggaa
     4441 gcgcggtaat accttcggtt tccgtggcct cgatgtgctc cagacgcgta aactcggcgg
     4501 caatatcagc ctcggatttg cccgccataa aatggcgcaa agaggtgatc gcctgtttac
     4561 cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc
     4621 acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac
     4681 ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc gttggcgctc
     4741 aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac
     4801 attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc
     4861 acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg
     4921 aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca
     4981 tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc
     5041 tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt aaccacgctc aataattgtt
     5101 tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactga
     5161 ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactata
     5221 ctacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa
     5281 ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc
     5341 taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa
     5401 tttaccgcga acttcataaa accagttaag gattgcaggg ggtaatggcg ttacagaacg
     5461 cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacagcg cagtggcaac
     5521 tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg
     5581 cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagaccgg
     5641 ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct
     5701 aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa
     5761 agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct
     5821 tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt
     5881 gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac
     5941 tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg
     6001 tcggtgtcat catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat
     6061 tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact
     6121 gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg
     6181 tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc
     6241 tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc
     6301 acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg
     6361 cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc
     6421 agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa
     6481 atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac
     6541 tatgccggaa gagtcttacc tctacgccct gccgtacaac ctgtacaaag agcacggcat
     6601 ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat
     6661 gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc
     6721 cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga
     6781 aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca
     6841 cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct
     6901 gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa
     6961 agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat acatcggtgc
     7021 ctacactgcg ctgatggatg gtcgtctgga cgctgttgta ttcactggtg gtatcggtga
     7081 aaatgccgcg atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt
     7141 tgatcatgaa cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg
     7201 tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag
     7261 ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc
     7321 caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc
     7381 ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa
     7441 ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc
     7501 gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg
     7561 aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag
     7621 atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt tgaaggtctg
     7681 gtcccgacac gtaagcacca gtttgcccag tctctgaact acgaaatcgc taaaacgctg
     7741 aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca gctgaaagag
     7801 cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat caccggcgtt
     7861 atcgtcaaca aactgaacgc accggttgat gaacagggtc gtactcgccc agatctgtcc
     7921 gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa
     7981 gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct gatcgcgact
     8041 cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat
     8101 actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggaacac
     8161 ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct
     8221 tgcctggcag ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa
     8281 atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt
     8341 atggtgaaca ccaacacctg gcagacctct ctgagcctgc agagcttcaa cctggaagtt
     8401 ccggttgacg atcacgagcg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac
     8461 gctgactgga tcgaatctct gactgccact tctgagcgca gccgtcgtct gtctccgcct
     8521 gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg
     8581 gaaggtgacg aaccgcgtac cgttaaagca gccgctatct gtgctgaacg tggtatcgca
     8641 acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta
     8701 gaactgggtg cagggattga aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt
     8761 cgtctggtcg aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg
     8821 gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga tggtctggtt
     8881 tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact
     8941 gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga acaggtttac
     9001 gtttacggtg actgtgcgat caacccggat ccgaccgctg aacagctggc agaaatcgcg
     9061 attcagtccg ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc
     9121 tactccaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt
     9181 ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta cgacgctgcg
     9241 gtaatggctg acgttgcgaa atccaaagcg ccgaactctc cggttgcagg tcgcgctacc
     9301 gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct
     9361 gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt taacgacctg
     9421 tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc gattcagtct
     9481 gcacagcagc agtaatctcg tcatcatccg cagctttgcg ctgcggatat ctgaaccgga
     9541 aataatcact atttccggtt ttttattctc ttaatctgca ttaatccttt ctgattatct
     9601 tgcttaactg cgctgcatca atgaattgcg ccatttcact ttgcatactt accactttgt
     9661 tttgtgcaag ggaatatttg cgctatgtcc gcaaccactg aatccaaacc aacaagaaga
     9721 tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac cagccttgcc
     9781 acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca ggttgatggt
     9841 caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gcattctgac taacgaagca
     9901 ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg
     9961 atgaacttcc cgtttgaagg attaacctca ggatcgaaat acgggacagc tgttggcatc
    10021 atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac aggaaccatt
    10081 gataacggta tcctggcgct tattcgccat acccgcggga atgaaattct ctttattcct
    10141 gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatgggaga agaggccgtc
    10201 gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag tattaccacc
    10261 gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat gaacccgttt
    10321 tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc cgggttgcgc
    10381 atcgtggtgt gggttatcgc cactctgatt ggcctgatct ttaccatggt gtacgcctca
    10441 cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa
    10501 aagcaggctg atgttgaaca acgtccgttt acctttggtg actggctggt attgattgtc
    10561 ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca
    10621 gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg cgttgttttc
    10681 cgccttaacg gcatgacggt taataccatg gcttcatcct ttaccgaagg ggcgcgaatg
    10741 atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat
    10801 ggtgaagcag gtgatgccag cgtgttaaat accatcctca acagcattgc caatgccatt
    10861 agcggtctgg acaacgctgt cgcggcctgg tttatgttgc tcttccaggc ggtatttaat
    10921 ttctttgtga catccggttc tggtcaggcg gcgttaacca tgccgttact ggcaccgctt
    10981 ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg cattccagtt tggtgatggc
    11041 ttcagccaca tcatttaccc aacctcagct tcgttaatgg cgacgctcgg tgtttgccgg
    11101 gtggacttcc gtaactggct gaaggtgggc gcgacactgc ttggactgct gtttattatg
    11161 tccagcgtcg tggtgatcgg cgctcagttg atgggctacc actaaaaatg ttaagagccg
    11221 caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt tttggcattg
    11281 cgcttcatcc acaacgccag cgctttcagc gagtctggag tgaactcatc gcagcgtgcg
    11341 gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg tagggcgaag
    11401 ggaccgtgag agacgcagct gaacaatgcg ccccagacac ggcaattttt atcttcgaaa
    11461 tagaactgcc cgtgctcagc aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg
    11521 cgacgcgcgg attccagcag ttgctcatcg gcctggacca caccgcctgc ggtcgcatct
    11581 aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat tttgcccatg
    11641 ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg catttgttcc
    11701 cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca ttcagtactt
    11761 gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct tacggcgcat
    11821 tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact ttgatcattg
    11881 agtgcgatta cactaagtac gtcattatcc agcatgccat aactcgccgg attaccgcct
    11941 ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg ttctgccact
    12001 ggcagatggg tatggccgta caccagcaca tcgttctggt ttaaagcagg cagattttcc
    12061 gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc
    12121 cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc gcagttgccg
    12181 cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt ggctggcgcg
    12241 taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag gatcaccagc
    12301 cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg taacgaccca
    12361 tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa acgtaagcta
    12421 tgatactgga ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg caataaaacg
    12481 ctggacagac gctcgctgcc actggaatgt cgcataatcc accaggcgtt ctggcacctc
    12541 atcgccatgt agtaccaggc ggttaatcat tagcgccaga tcagtatcag caatgcacca
    12601 ttcaccaaat aaattcggct gacccagtac taacaaatgt tctgccatcg caaacagttt
    12661 ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg caaagacaac
    12721 atccgtcgga cgctcttcgc ggatgggcat cagatcgctg cgcagccagg cctgaatctg
    12781 tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg tcggtggcgc
    12841 aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa aatcatcgat
    12901 ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggct gcaaatgttc
    12961 accgctgtcg aggtcgatgg tcttgatatg aaacgacagg cctttttcct gcaacgccac
    13021 ccaggcggat aacacataag gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg
    13081 tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag agcctttttg
    13141 tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct ttctatactt gtggtgttca
    13201 gatgattaaa caaccggagc tgcaatgatc gatctctatt tcgccccgac acccaatggt
    13261 cacaaaatta cgctgtttct cgaagaagca gagcttgatt atcgcttgat taaggtagac
    13321 ctggggaaag gcggtcagtt tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt
    13381 ccggcaattg ttgatcattc tcctgccgat ggcggcgaac cgttaagcct ctttgagtct
    13441 ggtgccattt tgttgtatct ggctgagaaa acaggactct ttttgagtca tgaaacgcgt
    13501 gagcgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg gccgatgctt
    13561 gggcaaaatc atcattttaa tcacgcagcc ccccaaacca ttccttacgc tattgaacgt
    13621 tatcaggttg aaactcagcg tctttaccat gtactgaaca agcggctgga aaactcgccc
    13681 tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg ggttaatgcc
    13741 tggactcgcc agcgaattga cctcgcaatg tatccggcag tcaagaactg gcatgagcgg
    13801 atccgttcgc gccctgccac cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt
    13861 tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa aacaacggag
    13921 aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt ttgcgtacgt
    13981 ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc aatgtgacga
    14041 tccactaccc cgccgataaa gcgcgcacta gcgaagatat caacgatgcg ctgaattatc
    14101 gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct ttgctggaaa
    14161 aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg tatgctgaag
    14221 tggagatcga taaactgcac gcgctgcgct acgccgattc ggtatccatg accttaagct
    14281 ggcagcgtta accgccatat cgggaggctg catgaacata gtgatcaccg gagggacggg
    14341 attaattggt cgccatttga ttccgcgttt gctggagctg ggccatcaaa tcacggtagt
    14401 gacgcgtaac ccacagaaag ccagttccgt tctcggccct cgggtgacgc tatggcaagg
    14461 gctagccgat caaagcaacc tcaacggcgt tgatgcggta atcaacctgg ccggagaacc
    14521 gattgctgat aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa
    14581 tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccgccgt cggtactcat
    14641 ttccggctcg gcaacgggct attatggcga cttaggtgaa gtggtggtta ccgaagagga
    14701 accgccgcat aacgaattta ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg
    14761 ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga
    14821 cggcggtatt ctcggtaaaa tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg
    14881 ttccggtcgg cagtatctgg cctggattca tattgatgat atggtcaacg gcattctctg
    14941 gctgctggat aacgacctgc gcgggccatt taatatggtt tcgccctacc cggtacgcaa
    15001 tgaacaattt gcccatgcgc tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc
    15061 cgcaaccgcc attcggctgt taatgggcga atcttcaata ctggtattag gtggacaacg
    15121 cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgctggtacg atttagaaga
    15181 ggcgctggcg gatgtcgttc gctgatgtgg tttacagcaa acatccgcca gttaactccc
    15241 ggtgttacag gattagtggc tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat
    15301 cataactaac tctctgtcta aacctctatc cagcatctcc tgagcaatac gcagggcttc
    15361 ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg tcatcagttt
    15421 ctccttttct tgtggtgcgc gttctgttat ctcaccaata aatgcacgga aacgctgggc
    15481 atcccctgtt tgtaatacgt aattaaacag ggcttttagc tgtctgtcat tagtgttccc
    15541 tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct gacgaatatg
    15601 tttctgaatt aactccaaca gcgccatttt gcggtgttgc ataatctcgt catccggcac
    15661 cacggtaata tccaccaacg gaaacgctga tgaatatatt ttgcgggcta tagcaggctc
    15721 ggcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac catgataaaa
    15781 cagcatcggg atcaccaatg gaagctcttt atagcccgca tcaagatggt tttgcattgc
    15841 cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac tttggtgctc
    15901 tatcactaca taaatataac ccactccctc ctgcgttttc acagaccaca agaggtcgga
    15961 ataatattgc cgcaggtcgt catcaataaa actgtttggt tccagtttaa gcgtcgttaa
    16021 atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc
    16081 tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg tcgatattgt
    16141 catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg attaacaccc
    16201 aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt agcggactgc
    16261 aacttttaca tttcagcgga agctgactca caaattcccc atgaaaatac tccggcagcg
    16321 ttagccaccg gagaggaaat tatttcagcg atcccttaag gaactgttgc agacgagggc
    16381 tttgcgggtt gccaaataac tgctccggcg cgccctcttc ttctattttt ccctgatgga
    16441 ggaaaatgac gtgagtagaa acatggcgag caaagcccat ttcgtgagtc actaccacca
    16501 tggttttccc ctcttctgcc agttgctgca taatacgtaa cacttcgcct accagttcag
    16561 gatcgagcgc cgaggtaggt tcatcaaaca gtaaaacttc cggttccatc gccagcgccc
    16621 gcgcgataga aacacgctgt tgctgaccgc cggaaagatg caccggatat ttcccctgcg
    16681 cacgttcgtc tatcccgact ttcgccagat acttcaccgc ccgctcgcgc gcttcctgct
    16741 tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc gtcatatggc
    16801 tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat
    16861 ttttatcggc gactttgagt tgaccatctt tgtcgcgcac cagattgatc gtctggccat
    16921 tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac
    16981 ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc gcttgcagtg
    17041 agaccccttt cagcacttca tgttcgccgt agcgtttgtg caaatcgata acgtttaatt
    17101 tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt
    17161 ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata gagcaccgcg
    17221 gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg ggctattttc
    17281 agcagatccg gcacagtggc cgtaaacgcc agcgcagtag agtgcagcat caggatcact
    17341 tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat gcaacgatac
    17401 attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg cggaaccgaa
    17461 cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc
    17521 acggtacagt tcaggccact gcggaagaaa gcgttaagga attcggttcc cttaacaatc
    17581 tcaagcgtgt acatgccgga atagaacacc agcaactgaa catacagcgg cgtaccgcga
    17641 aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga gacacgacca
    17701 atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag cagccacaga
    17761 gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt ccagtattca
    17821 tgtaagattt cgatcacagg tcagccctct tcacacccac ggagtagcgg cgctcaagga
    17881 acagcagcac accattggaa acagtggtga aaaccaggta aatcacgcca cagacgatgg
    17941 cgaaatagaa cggttcccag gtacttttgc ctgccagttg ggtggctttg accacatctt
    18001 ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag ttgttgccaa
    18061 tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac acttgcccac
    18121 gagtaaaacc gaacgccgtc gccgcctcta tatgcccttt cggcactgcc ataaacgcgc
    18181 cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtaata ataccagcga
    18241 ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg tttagtgcaa
    18301 tctgcaaacc gtagaaaatc agcagcatca acaccaagtc cggcacgcct cgaatcagcg
    18361 tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta ccgccagcgc
    18421 caattaaacc gatgattaca gcgagcacta cagaactgat agccagctcc agcgtgacga
    18481 gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg
    18541 tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc tttctttcac
    18601 cgatggcgat taaccgccat aaacatcaaa atcgaagtac tttttcgcta atttctcgta
    18661 agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc
    18721 ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt taacagacgg
    18781 gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc tggcagcgac
    18841 ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg
    18901 cccctgatac gagacgattt caatgccttt tggtgcccaa tgttcattac cgaacgtctc
    18961 ctgggtggtg ccctgcaata cgcctacccg tttgcctttc agcgactcga ctgtcggctg
    19021 aatgtcggaa tttttcgcca ccaccaaacg agaatcggca gcgtacagtt tgtcggtgaa
    19081 ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt
    19141 cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg tacattgcgt
    19201 attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca gttcgccttg
    19261 tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg
    19321 aatcgcagca aacgccgcag ttgcgctgga gaaggccaga accagagaga gcgatagcac
    19381 cagttttttc ataatgtcct caactgacag tctttttata gggaatattt acaggtttgt
    19441 tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc gttagacgta
    19501 ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag tgcaacaaac
    19561 ctggcgtgca ccatgatgat cattccatca ggtacagctt cccagcgacg tatcagtcac
    19621 cgtagacatt aaagtcgaaa tactttttcg ccatcttgtc gtaggtgccg tcctgacgca
    19681 gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta cgtagcccta
    19741 caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca aaggcgaaat
    19801 ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatct tgtaacgcag
    19861 catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca taggccacca
    19921 catccacgcc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt gatccctgca
    19981 gcacaccaac atgtttacct ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg
    20041 cagcaatcaa acgagaatct gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac
    20101 gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg
    20161 ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc cgtttgcaca
    20221 tctcgttacc gagatcgata tcaaagccaa caaaatcacc tttagcatct ttcgacgaga
    20281 acggcgcata ggtggtatcg gttccgatac gtaccgtctc cggtagcgcc gcatagctgg
    20341 aagccgctgc ggagagaccg actaacaaag acagagcgag aatcgacttc ttcatacata
    20401 accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt
    20461 tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa atatgttaat
    20521 aagacgttgc attattgtcc tgaagttgaa gatagcaggt atagcggttg aatcgcagcg
    20581 tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca aaattgccct
    20641 gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc ttcaggaagg
    20701 gttattgcaa actggtcaag aacacgatta accgtctgat ttatcacatc atcaagggat
    20761 tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat ttctgccgcc
    20821 tgagtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca gagcaccaac
    20881 gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc agtatagcta
    20941 tggacaatgc cggaaagggt tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg
    21001 aaagaaccgg aagagatgct ggcggaaata tcgcgcgcat cgtgcgtgac atcagctaat
    21061 gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg cgccgcctgg
    21121 ctcatcacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa taagcgcacg
    21181 ccataaatcg cgccgctggc accgctgatg cctacaatga gtcgtttcat aaatagttgc
    21241 cctgtcagac ttgcaggcag actttgcagt atttcggcgg gagttgcaag tcagggtgcc
    21301 agaccggcac cctcagcgaa ggcatcatcc ttcgttatgc atttcgagat tttccacttc
    21361 gttctgacgt tgcactgctt tggcgtcatc attacgtaac gtatcgagga aatcgaggta
    21421 gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt caaactgctg
    21481 gatatccgga ttttcagcgc gaacggcgtc gatcagatcg ttcagatcct ggaaaatcaa
    21541 cccgtcagca ccgatgatct ggcgaatttc atcaacttcg cgaccgtgag cgatcagttc
    21601 cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg gtgccgcaga
    21661 agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct gctcagaagt
    21721 ggtgccacgg acgatggagt cgtcgaccag caggacgttt ttatcgcgga actcggcgcg
    21781 gttggcattc agtttacggc gcacggactt acgacgcagc tgctggcccg gcatgataaa
    21841 ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc ccagaatacg
    21901 agcaatttcc agcgcgatat cacacgaggt ttctgggatc gggatcacca cgtcgatatc
    21961 cagatcttcc cattcgcggg caattttctc gcccagtttc gtgcccatat tcacacgcgc
    22021 gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata catactcaaa
    22081 caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact gcccttcttc
    22141 agtgatgtaa atcgcttcgc ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc
    22201 gagcgctacg ctttcggaag cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt
    22261 tcccagtacc agcggacgaa tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat
    22321 aatcatcgcc acacaggcat atgcgccgcg gattaagcgg tttgtggcag caatggcagc
    22381 gaaaatattg tcggcttcca gcgggtagtg gcggaagttg tccagctcgc tagcgaagat
    22441 attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt cttcaaacag
    22501 ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg taatgccata
    22561 cggagagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag ccgtagggta
    22621 acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag cttcaaatac
    22681 atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat ctatggtgat
    22741 gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg
    22801 gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt ttcctcgtta
    22861 agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt gcagataatc
    22921 aaaaaaccat ctgatgataa aactgaattg cgggatcagc tgtgatttgc tccagtcttc
    22981 gcttttcgac accccggtaa aggagtcgag aaagaagaga atggcggcaa caatcaacac
    23041 accgcgcaac gcaccgaaac agacgcccag cacccgatcg gtgcctgaca accccgtttt
    23101 ctccaccaac tggcctatca cgaagttcac gatagcacca acgatcaggg tagcgataaa
    23161 cagtaccgcg atggcaattc catttcgaac cagttcgtct tcaaagcccg taaaccagac
    23221 tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg tcaccagcga
    23281 taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag caatcaccgc
    23341 gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc tgtcatcctg
    23401 ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat
    23461 caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc tttgcggcat
    23521 cgtgcaatgc gtgtcggatg cggcgtaaac gccttatccg acctacggtt ctaacactgc
    23581 ccaggcctga taagacgcgc cagcgtcgca tcaggcaaga ctgtattaat tcggcgtata
    23641 gcccattacc acgccactta agccagaaag ttgcttcaac tcacccagcg aacctttcag
    23701 cttatctttc gaggcatccg gcccaaccag aatacgggta attttaccct gcactggcgt
    23761 ggatggcgac gtataaaccc gataaccggc accgcgcagc ttaccgacaa tctcattcac
    23821 tttatcggca tttttcagcg cacccagttg cacaacatag gctttacccg ttggcgctgc
    23881 tttttcttcc acgaccggct tcggttctgg cttcggcgca ggtggtgctt caaccttcgg
    23941 tttaggcggc tccaccggtt tcgacttcgg tggggcgacc ggtgcaggtt ccggttcaaa
    24001 ctcggtgtta ttggctgcaa tagtggtcgg atcgagcgac ggtgctgcgg catcacctgc
    24061 ccgcacctct tccgctgcgc cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg
    24121 catcatatca ggctcatcgc gatcgcccgc tttcggcacc agcgggatag ccgcgaactc
    24181 atcctgataa tgttttttct gcccgtccag cagccctgga agtacaatca cccccagcgc
    24241 caccagcacg atcgtgccca ctaaccgatt ctgaaactta cttgccaccg cttctcctcg
    24301 cgtcaatcac ttccatgaca tgtgcgacag tgtggaaaga accacacacc agcacggtgt
    24361 cttccgcttt agcgtccgcc attgcggcat cccatgcctg cgcaacgcta tcaaatgatt
    24421 tgccgttacc caaatgctca agcagttgtt ctgccgtggc accgcgcggc ccttccagtg
    24481 gcgcacaata ccagtcatca accacgcttt tcaaccaggc cagagttccg gcaatatctt
    24541 tatcatgtag cataccgata accgccagca cgcgcccgtt tttcggtagc gctttcatac
    24601 gcccggtgag atattccgcc gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg
    24661 actcgctcac aatctggaaa cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg
    24721 cattttcact gacttccagc ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg
    24781 gttgcgggac aagcggcaac ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc
    24841 agtcatgatc ggtgacggaa tagttccact caacgccccg acgttgtaac agtgcacctt
    24901 tttcctgtgc cacatcagca atggtagaag gcatttccgg ctcaccgaca attgccggtt
    24961 tttcgctgcg gaagatgcct gctttctcgc gaccaatact ttcgcgatct ggacccagcc
    25021 agtcggtatg atccagcgca atactggtta ctaccgcgac atcggcatcg acaatattgg
    25081 ttgcgtccag acgaccgccc agccctactt ccagaatcac cacgtcaagt tgtgcctgct
    25141 tgaacagcca caacgccgac agcgtaccgt actcgaaata ggtcagggaa atatcaccgc
    25201 gtgccgattc aatctccgca aaagaggcgg tgtgggccga ttccggcaat tcctggccct
    25261 gcacacgtac gcgctcggta taacgcacca gatgcggtga actgtagacg cccactttgt
    25321 accctgccgc catcagaatc gactccagcg tacggcaggt ggtgcctttg ccattcgtac
    25381 ccgcaacggt aaacacaaat ggcgctggtt tcagaacgcc aagacgcgcc gcgacctggc
    25441 tcacgcgctc aaggccgaga tcgatagttt tactgtgcag gttttccaga taagaaagcc
    25501 acgaagccag aggcgacgcg gcttgaggag tgcgtttgat aatcatggta tccgctgatt
    25561 cgttacggtg agaatagcaa aagggcagag ccagtggccc tgcccttatc agttatcagg
    25621 cctcaggttc ctgatccggt accgggggta ccactacgcc ttcacgcggc gcttcaggat
    25681 tcggcgctgg cagattcatc aacttcgcca gaatgctcgc cagtttcagg cgcatttccg
    25741 gacgacggac gatcatgtcg atcgcgcctt tctcgatcag gaattcactg cgctggaatc
    25801 ctggcggcag tttttcgcga acggtctgtt cgataacacg cggaccggca aagccgatta
    25861 acgctttcgg ttcagcgatg ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc
    25921 ccatcgtcgg gtcggtcagc acggagatgt acggcaagcc gcgctcctgc atttttgcca
    25981 gtgccgcaga ggttttcgcc atctgcatca gcgacatcag tgcttcctgc atacgtgcgc
    26041 caccagaggc ggagaagcag atcagcgggc agttatcttc cagcgcctgc tcaacggcac
    26101 gcacgaaacg tgcacccaca acagacccca ttgaaccgcc cataaaggcg aactcgaatg
    26161 ccgcagcgac aaccggcatt ccatacagag tgcctttcat caccaccagc gcatcttttt
    26221 cgccagtttc tttctgcgca gatgccagac ggtctttata cttcttggag tcacgaaact
    26281 tcagcacatc tttcggctca agctcgctac ccagctccac aaggcttcct tcatctaaca
    26341 ggctatgcag gcgattacgc gctgtcatac gcatgtgatg gtcacacttc ggacagacct
    26401 caagattacg ttccagctca gcgcggtata aaacctgacc gcagctatca cacttagtcc
    26461 acaccccttc aggaatgctc gccttgcggg tgggagtaat gttgctttta attcgttcaa
    26521 tccagctcat tagggacctt tctgtctgaa cctggttcga tgccagtttt atctttgggg
    26581 acgcataatg ccatttttgc ccccaacaga ccatgaatgt tgcacattaa aacataacag
    26641 cccgaaactt tggataaaaa agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg
    26701 tgccgcagcg cgtttgtgac gaataaattc gaaaacccca ggcaatattg aaacaaaaat
    26761 aatgccaaca atcatcagct taaggttgct ctgaatgaag gggagcgtgc cgaagaaata
    26821 acccgcgtag gtaaaaatca gtacccacaa cagtgcgccg atcacgttat aagcggcgaa
    26881 atgacggtac gacatgtggc ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg
    26941 tacaaaacgg gcgagaataa tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt
    27001 atcaagataa ctgcgacgaa agattttcga atccgggtta ctgaacagtt tgtcaccaaa
    27061 taaccgtcca atggtatagt tcactgcatc accgacaata gcggcaatca acataagcac
    27121 caccatcata tggacattga gatcgttagt ttccagcgac gccaatgcac cagcaacaaa
    27181 cagcagcgaa tcaccaggca ggaatggcgt taccaccagc cccgtttcgc agaacagaat
    27241 caaaaacaga atggcataaa cccagacgcc gtactccgcg accagctctg ccagatgcac
    27301 gtcaatgtgc aggataaaat cgatgaggaa ataaatcagg tccatatttg cctatgcctt
    27361 gtactcgtca tctttcaggc tgtaactacg ttggctacag cctgaaatgt tccgagcatt
    27421 attctcgtta gtccgccaga aatagcgggc ccattggcgg ttttggaaga tcataccggt
    27481 cagggtaatc caccgcgacc agatacagcc cttccgcttt tgccgttgct gccgcccgcg
    27541 ttctgtcctt tgccgccagc aactctgcta tccagctctc cggctggttg tgggcaccga
    27601 cttccatcag gctgccgaca atattcctga ccatatgatg tacaaaggca ttcgctttga
    27661 tatctaccac cacataagga ccgtgacgca tgacattaat atgcataacg ttgcgccacg
    27721 gggttcggga ctggcactgc accgcacgga acgaggtgaa atcattctcg cccagcaagc
    27781 attgcgcagc ccgatgcatc cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt
    27841 tactcagtac cgccgggcgc agccgatgat tgtagatgat gtagcgataa cggcgagccg
    27901 tggcgctaaa tcgggcatga aaatcatcag gtacagcttt aacccaacgc acggcgatgt
    27961 caccaggtaa attcgcattt acgcccaagg tccacgccgc gtctttgcgc tgcgcggttg
    28021 tttcgaaatg caccacttgc ccggtaccgt gtacccctgc gtcagtacgc ccggcgcaga
    28081 agacggtgat gggttcgttc gccacctggg agagcgcctt ttccagcttc tcctgcacac
    28141 tgcggacttc attctgccgt tgccagccgt aatacttact gccgtcgtac tcaatgccca
    28201 gcgcaatttt ataaactggc agttgttgct ggtcggacat tagtacagat actcctgcac
    28261 cagtttctcg gcgattttta ctgccatcag cgcgccgcca aagcgaacgt tatcggccac
    28321 cgaccagaac tggacttgct ccggcatacc gtagtcatta cgcacgcagc caacagaaag
    28381 atgcggcgta cccgaagcat cccctacctg agtcgggaat tcgttctctt cagagagcac
    28441 aatatcttcg ccctgagcaa acgcatcacg cgcttcttct gccgccagcg gacgcagtgc
    28501 ttcaaagttg accatctggg catgaccgta gaataccggt gcctggacga cgcttgccga
    28561 aatcatcagc ccttcgtcct gcaggatttt gcgtacttcg tcaacgatac gacgttcttc
    28621 acgcacgcta ccttcgctat ccggcaacaa cggcagcata ttgaacgcca gttgacgacc
    28681 gaagaaatct tcttcgtcaa tcggaatgcc gttgagcaat ttcgcactct gccccgctaa
    28741 cgcatcgacc gcttttttgc cctgggcgga ggctgaaatc aggctggtaa cactgatacg
    28801 cgacaaaccg ccctgatcga ttaacggttt cagtgccgcc agcagctggc tggtcagact
    28861 gtctggtacg gcgatgacat tccggttgcg gtaatcggtc agtacaaacg ggtttacttc
    28921 cggcaccacc agcggtacgt cgggttcgag agcaaacagt ccgctgctgt cgatcaccag
    28981 gcaacctgag ttggtcgctt cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa
    29041 aaatgccagc tgcgcctgcg tccagtcgaa ttcagcggca tcctgcacgg tgattgtttt
    29101 accaccaaag cgcagttgtt cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc
    29161 aaccgggaac tgacgttcag ccagcgtttc aagcagggct tcgcccacag cgccagttgc
    29221 gcccaggacg gcaatgttcc agccttcaga catggtggtt tactccagaa atagcaaagc
    29281 tccctgccaa tgttacagca gagagcatga agaagagatt aacgtgccgg atgatgaacg
    29341 gcgttaaaac ccagtttaca cagcaatgat gccgcactgg cgtcatcaca aattacatac
    29401 agagatgacc attcacggcg ctcaagatag tttttgcgca gtttatcgaa ctcacccggt
    29461 atcccggcga ctttacgcag cggtgcgtca tcgcggcgca catcatacac caaatgcacc
    29521 agccttttca gcgtcggttg atcgagcggg ccatgcagcg taatgcgacc aaactctggc
    29581 gcgggcagta atgtatccag cgcaacgtgc tgttcatgcc caataaactt gctataggct
    29641 tcaaacactt gcgtagtacc gcgtgcttta ccttccaggg tataacctgc gatatgcggc
    29701 gtgccgatat ccacttttgt cagcaactcc acgttaagtt ccggttcgcc ttcccagaca
    29761 tccagcacta cgcttaactt ctggccttca ttcaggcagg tcagcaacgc agtattatcg
    29821 acgactgcgc cacggcaggc gttaatcaga atcgctcccg gcttcaggct acggatcagt
    29881 ttttcatccg ccagatgcag cgttttgtac ggcccatctt tgaagagtgg cgtatggaaa
    29941 gtcagaatat ccgcatgctg aactaattca tccagcgagc ggaaatcacc ctcatcccca
    30001 cggtcagcgc gaggcggatc gcaaagcaat gttttaatcc ctaacgcttc cagtcgcgcc
    30061 tgcaatcgac gtccaacgtt accaacgccc acgatcccca ccgtacggtc gtgcagtgaa
    30121 aatccatcgc gttcggcaag catcagtagt gaggagaaaa catattccac caccgcaatc
    30181 gcattacagc caggtgcagc ggaaaaacca attcccgcct gcttcaacca tgcttcatcg
    30241 acatggtcgg tccccgcagt ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa
    30301 gattcattca ctttcgtgac cgaacgcacc atcagcgcat ccgcgtctgc cagttgagca
    30361 acggggattg gacgcccggg aaccgcggtc acctcaccca aacggctaaa taagtcgcgg
    30421 gcataaggca tattttcatc aacaaggatt ttcacgtttg tgttacctgt atgagacgag
    30481 agttaaccgg acaagtgtgc cataatctcg cggccaggca tacttgcgaa gatttcaggt
    30541 ataaggatac gtaatgatac aacctatttc cggccctcct cctgggcaac caccaggtca
    30601 gggagataac ctgccgtctg gcgcgggcaa tcagccttta tccagtcagc aacgtacttc
    30661 gctggaaagc ttaatgacga aagtgacctc actgacgcaa caacaaagag cagaactgtg
    30721 ggcgggtatc aggcacgata ttggtctgtc gggagattca ccgctgcttt cgcgtcactt
    30781 ccctgccgct gagcataatc tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc
    30841 ccgccagctt ttagcgcaat taggggagta tttacgtctg gggaataatc gtcaggcggt
    30901 cacggattat atccgtcata actttggtca gacgccgctg aatcagctct caccggagca
    30961 attaaaaacc attctcaccc tgttgcagga agggaagatg gttattccgc aaccacagca
    31021 gcgcgaggcg accgaccgtc ctttattacc ggcggagcac aatgcgctaa aacagctggt
    31081 gaccaaactt gcggcggcaa cgggggaacc cagcaaacag atctggcaat cgatgctgga
    31141 actttccggg gtgaaagatg gcgagttaat tccagcgaaa ctgtttaacc atctggtgac
    31201 ctggttgcag gcgcgccaga cgttaagcca gcaaaatacg ccgacgctgg aatcactaca
    31261 gatggcgcta aaacaacctt tagatgccag tgaactggcg gcgttatcgg catatatcca
    31321 gcaaaaatat ggcctttctg cgcaatcatc gctttcttct gcccaggccg aggatattct
    31381 taatcagctt tatcaacggc gggttaaagg gattgatccg cgtgatatgc aaccgctgct
    31441 taatcctttt ccaccgatga tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg
    31501 gatactgtta gtcgcgatta tcctgatgct ggtctggctg gttcgttagc cccgacgaaa
    31561 tgacagtatc gtgacaataa ttcccagcac cgcagagatc gccccggcaa gaaataccga
    31621 agagtaacca aacgtggtcg ccagcattcc cgccagcggc ccggagacgc cgagggcgat
    31681 atcctgaaac gcggcgtaac cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt
    31741 aaccacctcc acgcccagcg caggaaagat aagcgaacat ccggctccgg ttaacgccgc
    31801 gcccgctaat gcgacccacg cacctggggc ttgccagagc agcaacaagc ccaccgtttc
    31861 tacaagcaga gagacaatcg ccactttcac gccgccaaaa cggtccggca tccagccaaa
    31921 catgacgcgc atcacgacaa atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc
    31981 ccatcctttg ctggcaaagt agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc
    32041 ttgtagtgcc agccctaacc ctggtttcca gataaacccg acaacgctcc acagcgatgg
    32101 acgttctccc gccagggccg gtactttgcg cactgtgccg ttacaggccc acgccagtaa
    32161 gggtaatacc attgtggtga tcgccagtgc ggcaaaaccg taatggctat gaatcaacag
    32221 gccaagcgga gcaccaacag cgagggcacc gtaaatcgcc attccattcc atgacatcac
    32281 tttgccagag tgttttggcc ctacgatgcc taacccccag gtcagagcgc ctgtcagtaa
    32341 ctggctttca ccaaacccaa gaatcaaacg cccgatgacc aacagggcaa atttgaacgg
    32401 tgcggagaca ggcaaaatcg ccgccagcag caacgcgccg ccagccagac cacaagctaa
    32461 catcccctga agcgccgaac gttttgcacc atattgatcg gccagtcgcc cggcatagcc
    32521 acgcgtcagc accgtagcca gaaactgaat cccgacggca atcccgacca tggtattgcc
    32581 atagcccaga tcatgatgaa caaacagcgg gataaccggc aacggcagcc ctacggtcat
    32641 gtaggtgaga aaaaccgcaa aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt
    32701 ttcggtttgg cttacagcag tcatgcatta ctccagaatg cagcgcaagg cgaggagtat
    32761 ccccgtctca tctctctggt ttcagggtta cggtgcgttg gcaggattta acgcgtacgt
    32821 cttttcagaa ggaaatcgac aaagcgggaa gtttgcctgg aacaggcggc gattgtcaat
    32881 gatgtgaaaa agggaaccat caggttccct tttgcgttag tgccggaggc cgcattagcg
    32941 cgtaacgtcg gatgcgacgc tggcgcgtct tatccgacct actccgacta ctccggccta
    33001 ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg ccgaagccga
    33061 agctgttaga cataacggtg gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac
    33121 ctgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag ccgtgttcca
    33181 gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag tgaccggtca
    33241 tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg atagctgcca
    33301 gttctttcac gtcgccaacc ggagtcgaag taccatgaga gttcaggtaa tcgattgggg
    33361 tatcaacgcc gtgcatcgcc atcttcatgc agcgtactgc gccttcgcca gacggagcaa
    33421 ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag atgtgagcac
    33481 cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg ccagcgataa
    33541 cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt
    33601 tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac agctcttcgc
    33661 cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact gcgttaccga
    33721 tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca tgaattttaa
    33781 acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc acatacgggc
    33841 caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaacacc tggaaacgcg
    33901 gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg taagcttccg
    33961 gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa atggatgcgt
    34021 cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc
    34081 cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg atccctgaac
    34141 gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg gaaacaatgc
    34201 ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac atagagtaag
    34261 tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc catttaatag
    34321 agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt aagacgagta
    34381 acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc tgagggtaca
    34441 cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct ggaagagacg
    34501 cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca tccacatcct
    34561 ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac gctatggcag
    34621 gcatttaatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt acatttcatt
    34681 agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca acactggccg
    34741 gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt gcccggttgc
    34801 catcgtttat tgctcgatga aggccgcgtg acgctggatt tatggtttgg cgatattaac
    34861 gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg gtttctggac
    34921 ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa cgccatggca
    34981 aggttggcgc gtccgggcgg cacgctggcg acatttacgt ctgccggttt tgtccgccgc
    35041 ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg gctttgggcg caaacgggaa
    35101 atgctttgcg gggtgatgga acagacatta ccgctcccct gctccgcgcc gtggtttaac
    35161 cgcacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc cagcgcgttg
    35221 ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc tttattgcgc ggatgaggcc
    35281 cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt aagcaaacac
    35341 gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg gttttacgac
    35401 caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg ctgggatgag
    35461 aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga actggctgta
    35521 gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg cagcggcatt
    35581 acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt gctggaactg
    35641 gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt atcccgtaag
    35701 gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag cgtagtggta
    35761 ctggcgaacg ggcatcaaat cagccgattc agccaaacgt cgactctccc ggtgtattcg
    35821 gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct gaagcaggtg
    35881 ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca ttgtattggt
    35941 gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca gcagcagaat
    36001 cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt tgatgtcagt
    36061 gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc aatggtaggc
    36121 aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga gcagaaagat
    36181 gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt aggttctcgc
    36241 ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag cgacgaaccg
    36301 attccgatgg atgccagtac actggcggcg ttaaacccga atcggttatg ggtgcggaaa
    36361 ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt tgccggatgc
    36421 ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac gcgataagcg
    36481 tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct gctgaaacag
    36541 gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac cggcaccaat
    36601 ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt ggtcatcacc
    36661 actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac tggcaaacaa
    36721 ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc gtgattcaaa
    36781 ctccgcgatc atcttctttc ctcattgtaa aacgtggcac cggcttcagt tgagcgcttc
    36841 cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt gtagtgcttt
    36901 aatgaagcgt gccgggcgtt caggaatgcc gtttgctagg taatccatca cctgcgcatg
    36961 tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg ccttccagat tgtcgcaact
    37021 gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgaacgcct gcggcttcac
    37081 ttcaacatct tcaaactggc tttgcgtttg ggcatcgcgt ccgtccgggc aataccagta
    37141 gccgaaatca accagctcac ggcgcgcttt cccagcaata caccagtgcg aaatctcatg
    37201 aattgcgctg gcataaaagc catgagcaaa gacgattcgg ttatacggta cttccgcatc
    37261 agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa attcatcggc
    37321 aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt tcattagttc
    37381 atccccaacc agtggaggat ctcctgtccg tggctatcaa aaagtaattt ggcactcatc
    37441 accgctgaga caataacaat catcgggcgg atcagctttt gtcctttgct caacaccagt
    37501 cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc cgtcgcccaa
    37561 atcactttgc caccgagaat aaacagcagt aaaccgccga tgtttgacgt tgcgttgagt
    37621 aatttggcgt gcgccgtggc tttggcgagg ttgaatccgc acagcgtaac aaaggccagt
    37681 gcgtaaaacg atccggctgc cggaccaaag aatccgtcat aaaaaccgac gcagccaccg
    37741 gcaattaacg cgaagggtaa accgtacatc cgacgctggc gatcttcttc acccagcttt
    37801 ggcatcagca agaaatagag gccgatacaa attaccagaa tgggcaaaat ctgccgtaag
    37861 acatcagcct gaacgtattg caccagcagt gcgccgctca ttgagccgac aaaggtcatg
    37921 gcgatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa gtagatagta
    37981 gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcatt agcgggagac
    38041 atccccgctg ccatcaatgc cggaatggtg agtaacccac cgccaccggc aatcgagtcg
    38101 ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg ggaaaccata
    38161 aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc tggcaggaag
    38221 gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt ggaggttcaa
    38281 accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt tgatcttcac
    38341 actccagact atcggcagga caacgtaatc gtacatgcat atgcgcacga tgctggaacc
    38401 agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atctaggcaa agttgttgtt
    38461 taatcgccgg attaacaaaa atgcgcgtga cgtttttgtc ctgggcggcg agtttgatca
    38521 agctgaaaat ttctggcttc cacagcgagg agacaacgtg tttaccgtcg cgggaaacta
    38581 agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc ggcagttgca
    38641 gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg aaacgcccac
    38701 cagcaggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg ctcacctggc
    38761 tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga cgctgatcgg
    38821 tacgcatgac ctgataatgt tcggactgta tcggcagcgt gtcagcgccg acaatacagc
    38881 cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt attttttgcc
    38941 acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca atcgcggttt
    39001 tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc gcccgctgcc
    39061 gtaacaggtg atccattaaa acgatcgcca gcatcgcttc tgcgatcggc actgcacgga
    39121 tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg ccaaagcggt
    39181 taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata tgggcaatga
    39241 tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc tggaaaccgt
    39301 ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca aaaccgtcgc
    39361 caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg tcggcatcca
    39421 ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca acggtgactt
    39481 tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct aacgcgtcga
    39541 ttttgtccgg gtccgggcag aagaacggat tttgttcaac cagcgaccag tctttgattt
    39601 ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca aatttctcgg
    39661 cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg cgggcggaag
    39721 aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg taatcagcat
    39781 ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg tcagtgtttt
    39841 cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg gagagaattt
    39901 tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg cgacgacggt
    39961 cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca ccatcgacga
    40021 tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa
    40081 ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac ttaatcttta
    40141 taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat aaacacacca
    40201 tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg ttccataaga
    40261 tgtaccatgc tgttgccgac ttcacaaatc agcacaccat catcagcaag gtaatctgcc
    40321 gcgttaccga gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc caggcccagt
    40381 tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc gacatacggc
    40441 gggttagtga caatcagatc gtattgcact ttcggcaaat cacggaacag atcggaacga
    40501 atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc aaccgccagc
    40561 gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata agcacaggca
    40621 atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg cttgctgatg
    40681 agtccggcaa atttattgtt gatcagttca ccaatcggtg agcgcggcac cagcacgcgt
    40741 tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta agccaccgga
    40801 atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc gctggaggtc
    40861 agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga tggtaacacc
    40921 agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat atttgccgcg
    40981 ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc atttactgct
    41041 tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata atttcggcgg
    41101 ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg aggaaaaata
    41161 cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt agaaatagag
    41221 acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg tttcgccagt
    41281 tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg cagcgtaaaa
    41341 aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc agccattatt
    41401 tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat gttcgcccgg
    41461 atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg gaattgtttt
    41521 tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg ttgattgccg
    41581 cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg aagcatattt
    41641 tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca tttcaccagg
    41701 caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg gaagagtggt
    41761 tgccgccgga gttgccctga ggagttgagc gtgttgtgcc aggagcgcgg aatggcaggg
    41821 gcgaatgacc ctgccaggtg agaaaaggtg cgctaataca tattcagtat gtaagtgaat
    41881 cggcaggtag aatctatggg ctgtcgagat gtcgtgtcca cgcgtagctt catatcatgc
    41941 agttgcctgg acgtgatcgg ttgcggctct ggcgtcaccg tcactttggc attatcacag
    42001 ccggaagtcg agaaggtcat ctgctgtgcg acaaaacggc agtaggctcc acgctcgtca
    42061 aaattactgc catcacacag tttgccgttt tccatcagga aagtgggcag tgtttgctca
    42121 ataacaccac ctgttgactg caacctgacg actttgttat gtacaacata attttcattc
    42181 taggaaacat catacggtgc cagggactct gtcgggcagt cagagctaac ttttgttggt
    42241 cctataaaaa ttccggcccc gaaacgctga tccatagtac tggcgctttc aacataagac
    42301 gacacaatcc agggacaacc gtgtaaatct ccatcgtaat aaacgccaag tactaattca
    42361 ttacatgacc aggccgttac tggagagaga gcatcatact cttctaatcc ggggcaggta
    42421 gaatccggta aggttgtatt attgcgctcc atcctgagtt cagggccggg aggactcccg
    42481 gtccaactaa ctaaaggcaa acttgctgcc gcagttgaat tagattcata cgtgccatat
    42541 tcaactgatt gaggggtcag gactgaaaga tgaaaacgat actttgcaac aacgctggtc
    42601 gtccccgcgg gtacatagag tgtggcgatc tttgtcgcag cctgtatccc agaagcgcac
    42661 agagccagca ccaataaaca tataacatga cgtattttat gcattttata tatactcaaa
    42721 cgtagctgtc aggctggcgg tgaaatcacc gatggttaca tcacgaccgc ttttcgcctg
    42781 gatccaggcc cggaaattaa tatcattgtt gccgttggta agcgcaaaag tcgccccggt
    42841 ggtgttgtta atatccaccc gcttgccgtc gagcgtttcc atccctatcc ccacgccctg
    42901 cgcggtggag gtgtcgtcga ctgccagaaa acccggctga ccgctgtctt ccgttccggt
    42961 gagggtgact ttgacgttgt atcccgccgg ccctttgcag tctttcagct taaacacaac
    43021 cggcagatgc gcggactgcc cggcgaccat caaatcccgg ttgctgacgg tgggaaaatg
    43081 aatttccgcc agattggcac catccaccac cagcgtgcag gatttactca gcaggttgcc
    43141 gtaaaaatgc aggttgttat cgactgcccg tgcccccgtg ctggcggtca gcagcaggcc
    43201 tgctaacagc gcataaccgg gtgaataaat gtgtgctgtt ttcattggta atccacccgt
    43261 aaggttgcgt aagcagaaaa attcgtgtcc gtcagcaccg catcagccgc tttgaccggt
    43321 accgcctcca gctttggctt ctggctaaaa tcggtggcgt taatggaaag cggctcgttg
    43381 agcttaaaac gctgaccgtc atgctgaagc tcaattccga agcccggtac atccgtctca
    43441 atagcagagt cgttgtagga ggtggcagtc cccgtccagg agagtgtcat attccactcg
    43501 ggatcgcgga catccgagtc gcagctcagt tcgtagtcca ctttctgacg accataattg
    43561 ccatcaatgc tgtcgataat aagatcgcga aattgcactt cgatggtttg cccgccgctg
    43621 atgctacagg cgggcggcga catcagcgta ccgtggaatg tgatgtcatc ttccgccgcc
    43681 agcacagccc cgcaaccagc gcacagtaaa aggagcagga attttttcat tggtaatcca
    43741 ccaccatggt catcgtggcg ttaaactccg acggcgtcag ttgcaccccg ctctgtttta
    43801 ccggcaccgc tttcagctga ggctgagtgt tgacattaaa aggcgtccag ttattttcac
    43861 caactgcaaa cagggagttg tcggcggcgt tttctatccg cacccccagg ccggcgatat
    43921 ccgtgctgag aaccgtttct ccgttgatca cggcggtatt gcctttgagc tgcatccgta
    43981 agtcatcgct gactttatta ccgcagttga gggtgtattt cgcatcctgt tggtaattgg
    44041 taccatcgat cctgttgatt atcacattgc caaatttcac agcgcttccc ttcacggagc
    44101 aaggcggcgg tccatcgacc aggattcgca gcgtcagatc cacctgtttg atatcactgc
    44161 cagcaacagc caaaccgctg atgccccatc cggccagcac cagcaaccct gcgcccgcta
    44221 caaacttgtg actcataagt ccctcccggc atcagccttt gcggctctgc tcttcatcca
    44281 cctggcagct gttgccggtg catatgaagt acagcggcat ccgcgcgccg tagtcgttga
    44341 tgtaagtcag caccggcacg ctgtccatct tcgcattcag cggtgcggag gttttcggct
    44401 ccatcatcag cggtgtgaat cccgccggcg ggttgccgcc ttttttagcg ctggcgttgc
    44461 tgatgatcac gtagtacggc gtcgggttat tgacggtgaa gtcctggccg ttacggatga
    44521 gcgtcacctt atgctgccac ggacttttca tgctggtgtt ctccagcgcc ttcggtcgcc
    44581 agaacagttt gatacgcgtc tgcagcgcaa tctgcagtgt gttcggttta tttgatttcg
    44641 gcggaatttc gcgcacgttg aaatagaaca cgctctcgcg gtcggccggc agtttgttga
    44701 tatccggcag cccctgcacc ttcacctggc cgttcatcat cgcatcgata cgctgcactg
    44761 gcggaagcac cgcaagcggc gagctgattt tgttgccctt tgcatcctct atccagctct
    44821 gcgccagata aggcagtgtg ggatcgttgt tgcgcagcgt gacgctgatg gacttgtcgc
    44881 tttcgttaaa caccagccgt gtcctgtcgg gcgtcaccga agcgtggctg gagaaactga
    44941 ccactgtcgc cagcagcgcc agtgccggga caacgatttt tgcagtgtta gtcagattaa
    45001 acatggatca ttccttaatc acgttactgg tttactgaaa ttgaagtcgg aggttccgtt
    45061 ggcgtcaccc gctgtgtctg ttcctgaatc gccggtttga ccgccggcga agtgataggt
    45121 gctacattgc ctttatgctg gcatggcagt aacaagccgt taaacagatc ggcaggcagc
    45181 gggtccggca ggttgatatc gcaatgcgca acaccactcc agaacacact catgtgttcg
    45241 ccaggtttca ccccagccag ataaacattg ccgtcatcat cgacaaggcc cactgtctgc
    45301 tcgttatcat tttttacttc tgcgccaaac ggaggatggc tgccgtcgct caggcgcagc
    45361 accgccatcg ctttttgtcc gctaatgaca gcaaatttac ggtagccaat ggcaccttca
    45421 gttagcgtgg cttgcaccac cgactgggtt gcttcagcgt tttcaggcaa tctgttgagg
    45481 tcgatatacg cctgattgcg gtaatagtta ttgacgtcag aaacgacggc tttaccaaac
    45541 atattggtat aaacagcagc cccgttacct tccaccggaa catcggcaac gccatcggca
    45601 tcaatcaaca atcgtgtccc gcccatattc tgggtacggt gaagtgcgcc accgtgggca
    45661 gtaagcgttg cgccgccctg taacgacaag cccgcagagg tgtattgccc ttcatggtag
    45721 ttcgcactga ggtcaacctg cgccagcgaa ccatcatggc tgtaatagcc gtcaacgctg
    45781 gtgtgtttgt cactggtgcc gacgttcaac tgatagtgag tcgcgtcatc gacacggctg
    45841 aaataaccga cctgactgct gtccgtccca ctgccatagt taccgttata gctaacggta
    45901 ctgttgtcgc cccacggcat actgagcgaa atgtacatgc ctttgtcggc ctggttgtca
    45961 tactcgtaac ggtagccagt catcgagatg ctgacattac gaatactgcc catattgaaa
    46021 tagtgcgaga gcatgatgtt gtagtttgtc tgctcctcgc gatcccagta ggtatggcgg
    46081 gtatagttga gataaaccga aacacccaca tcgcggaagt tctggttata agtggcggtg
    46141 tacatctctt tgtcgttgcc cgtgcgtacc attccgctgt cactggcatc cagatactcg
    46201 ctcatggtca taaagttctc ttccgagaag cgatatccag caaaagtaac gcggctgttg
    46261 agctggtcaa agtctttgga ataactcaca cggaaggagt taccgtccag cgaacctttg
    46321 ccataagcgg tgtctttatc cagtttggtg tgcgagtgag taacatcaaa cgcgaccgcg
    46381 ccgaatgtag acaaatcgcg accgacgcca agcgccgcag actgatagtt ttcatctccc
    46441 agtgcgccgc catataacga ccagccgtta gcgatccccc aggaagcttc agcaccagaa
    46501 aaaaattcac cctcgatatg gtgtccccac tcttgcggac ggcccatcat gatcttatag
    46561 cgaacctgac ctgggcgagt gaggtatggc atcgaggcgg tgctgatgtc atattcctgc
    46621 acctggccgt tctgttcttc aatgcgaata tgcaacgtac cggagacaga atcaccaaga
    46681 tcctgaatac gaaacggtcc agccggaacc tgcgtttcgt aaatcacacg ccccatctgg
    46741 ctgacggtca cttttgctgt ggtgtgtgcc acgccggaaa tgtctggcgc gtagccgcgc
    46801 agattgggag gcaacatttg atcgtcagta ctgacactgc caccaacata gttaaagcca
    46861 tcaaaaatat cggatctgag gtaatcctcg cccagcgcca gtttggcttt taatgatggt
    46921 aacgcccgcc aggcatagta gcgactccac tcccattttt tttgagtttc atcgccgctg
    46981 aattcatcgt catcatcatt actgcgagta tgttgatagt tagtctgcca gtcagcacgc
    47041 atacgccacg gccccaggtt aaccccgacc gtcccgttgc cgctgatctc gttactatca
    47101 tcaccgccat tttcttcgtg ccgggtttgt gcgttgatgc tgtagtccgc gacgatcccg
    47161 gagatgccgt catcccaacg tgaaggcgga tcccagtcgg gataagtata ttcgaggtaa
    47221 gcctgtggca gtgaaatgac taatgcggac tggcttaaat cagccttaat ttccatgcct
    47281 tccagttgac cggatttcag gcatttagca tcgtggctcc attgcagatt tttcgccacg
    47341 tcttctttta aaccaaactg cgctaccagt tccggtgtca gacaagcata gcttttgctc
    47401 gcgtcatctt caccagcata ccagtaaata tcgtactctt ccgccaatgg ctgtttattt
    47461 agttgaacct gtaaattgta ttttccgggc tcaacatatc cctggctgga aaaacgcttc
    47521 agatcaattt ttgtgtcgcc ttttaattcc agaaaacggg aatcaaactg aatgtcgtct
    47581 tcagcccaga cactgctata actccctgac attgccagcg caatgcacca cggaagaatc
    47641 cgtaatcgaa aaagagaatg gtcaggcata cacgtttcta tccgttttta ttcttgttcg
    47701 gtcaaaatcc atttaaatcc cccatagcag tgtcgctatg ggggtatgcc ttacaggtaa
    47761 gtaatctgga aggtggtatt tgcttcgaaa ttacccagat ctggcgcatc agctgcgccg
    47821 accagccagg ctttaaagtt cagtgtctgc ttcgctttgc ctttattggt agaagtgtct
    47881 ttcactattt tctggtcaat acccatgccg cttttgtaag aggtacccag agagtcaccg
    47941 atcgccaggc taacattgtt aaatgccgca ccggtatcgg tattgaaaat ggtgtaataa
    48001 ttgccgctat ttgcagaaga aacggtacca gtaaaggtag tggtcattgt ttcctgagta
    48061 tcaaatacgc aatcttgcag acgaatctgg aaacttttcg gcgtggtggt gccgttattt
    48121 ttcagttttt cggcaccgat atcacccata tcaacttcca gtttgtgatc gtccggaacg
    48181 atagaacaca ctgagtcttc aatggtgccg taaaaacgtg cagtaccgtt gttacccgca
    48241 gcgattgttg ccgtagaggt gaaaacgccc atcaccattg cc
//
LOCUS       DAZFRJ010000011        47656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000011 DAZFRJ010000000
VERSION     DAZFRJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.011"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..675
                     /gene="comR"
                     /locus_tag="ACHKUN_000584"
     CDS             43..675
                     /gene="comR"
                     /locus_tag="ACHKUN_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO6658897.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(737..1276)
                     /gene="ycfJ"
                     /locus_tag="ACHKUN_000585"
     CDS             complement(737..1276)
                     /gene="ycfJ"
                     /locus_tag="ACHKUN_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6658898.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1503..2807)
                     /gene="ndh"
                     /locus_tag="ACHKUN_000586"
     CDS             complement(1503..2807)
                     /gene="ndh"
                     /locus_tag="ACHKUN_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO6658899.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3207..3749)
                     /gene="ycfP"
                     /locus_tag="ACHKUN_000587"
     CDS             complement(3207..3749)
                     /gene="ycfP"
                     /locus_tag="ACHKUN_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO6658900.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3772..4797)
                     /gene="nagZ"
                     /locus_tag="ACHKUN_000588"
     CDS             complement(3772..4797)
                     /gene="nagZ"
                     /locus_tag="ACHKUN_000588"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO6658901.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4808..5632)
                     /gene="thiK"
                     /locus_tag="ACHKUN_000589"
     CDS             complement(4808..5632)
                     /gene="thiK"
                     /locus_tag="ACHKUN_000589"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO6658902.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5613..6254)
                     /gene="lpoB"
                     /locus_tag="ACHKUN_000590"
     CDS             complement(5613..6254)
                     /gene="lpoB"
                     /locus_tag="ACHKUN_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO6658903.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6268..6645)
                     /gene="ycfL"
                     /locus_tag="ACHKUN_000591"
     CDS             complement(6268..6645)
                     /gene="ycfL"
                     /locus_tag="ACHKUN_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO6658904.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6648..7007)
                     /gene="hinT"
                     /locus_tag="ACHKUN_000592"
     CDS             complement(6648..7007)
                     /gene="hinT"
                     /locus_tag="ACHKUN_000592"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO6658905.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7341..9530
                     /gene="fhuE"
                     /locus_tag="ACHKUN_000593"
     CDS             7341..9530
                     /gene="fhuE"
                     /locus_tag="ACHKUN_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO6658906.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9590..11023)
                     /gene="ptsG"
                     /locus_tag="ACHKUN_000594"
     CDS             complement(9590..11023)
                     /gene="ptsG"
                     /locus_tag="ACHKUN_000594"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO6658907.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11318..12115)
                     /gene="ycfH"
                     /locus_tag="ACHKUN_000595"
     CDS             complement(11318..12115)
                     /gene="ycfH"
                     /locus_tag="ACHKUN_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6658908.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12126..13130)
                     /gene="holB"
                     /locus_tag="ACHKUN_000596"
     CDS             complement(12126..13130)
                     /gene="holB"
                     /locus_tag="ACHKUN_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO6658909.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13127..13768)
                     /gene="tmk"
                     /locus_tag="ACHKUN_000597"
     CDS             complement(13127..13768)
                     /gene="tmk"
                     /locus_tag="ACHKUN_000597"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6658910.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13758..14780)
                     /gene="yceG"
                     /locus_tag="ACHKUN_000598"
     CDS             complement(13758..14780)
                     /gene="yceG"
                     /locus_tag="ACHKUN_000598"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO6658911.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14783..15592)
                     /gene="pabC"
                     /locus_tag="ACHKUN_000599"
     CDS             complement(14783..15592)
                     /gene="pabC"
                     /locus_tag="ACHKUN_000599"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO6658912.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15712..16953)
                     /gene="fabF"
                     /locus_tag="ACHKUN_000600"
     CDS             complement(15712..16953)
                     /gene="fabF"
                     /locus_tag="ACHKUN_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO6658913.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17041..17277)
                     /gene="acpP"
                     /locus_tag="ACHKUN_000601"
     CDS             complement(17041..17277)
                     /gene="acpP"
                     /locus_tag="ACHKUN_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6658914.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17488..18222)
                     /gene="fabG"
                     /locus_tag="ACHKUN_000602"
     CDS             complement(17488..18222)
                     /gene="fabG"
                     /locus_tag="ACHKUN_000602"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO6658915.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18235..19164)
                     /gene="fabD"
                     /locus_tag="ACHKUN_000603"
     CDS             complement(18235..19164)
                     /gene="fabD"
                     /locus_tag="ACHKUN_000603"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO6658916.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19180..20133)
                     /gene="fabH"
                     /locus_tag="ACHKUN_000604"
     CDS             complement(19180..20133)
                     /gene="fabH"
                     /locus_tag="ACHKUN_000604"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO6658917.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20201..21271)
                     /gene="plsX"
                     /locus_tag="ACHKUN_000605"
     CDS             complement(20201..21271)
                     /gene="plsX"
                     /locus_tag="ACHKUN_000605"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO6658918.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21352..21525)
                     /gene="rpmF"
                     /locus_tag="ACHKUN_000606"
     CDS             complement(21352..21525)
                     /gene="rpmF"
                     /locus_tag="ACHKUN_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO6658919.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21577..22098)
                     /gene="yceD"
                     /locus_tag="ACHKUN_000607"
     CDS             complement(21577..22098)
                     /gene="yceD"
                     /locus_tag="ACHKUN_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO6658920.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22297..22881
                     /gene="yceF"
                     /locus_tag="ACHKUN_000608"
     CDS             22297..22881
                     /gene="yceF"
                     /locus_tag="ACHKUN_000608"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO6658921.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22993..23952)
                     /gene="rluC"
                     /locus_tag="ACHKUN_000609"
     CDS             complement(22993..23952)
                     /gene="rluC"
                     /locus_tag="ACHKUN_000609"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO6658922.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24525..27710
                     /gene="rne"
                     /locus_tag="ACHKUN_000610"
     CDS             24525..27710
                     /gene="rne"
                     /locus_tag="ACHKUN_000610"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO6658923.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27906..28859)
                     /gene="flgL"
                     /locus_tag="ACHKUN_000611"
     CDS             complement(27906..28859)
                     /gene="flgL"
                     /locus_tag="ACHKUN_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6658924.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28871..30514)
                     /gene="flgK"
                     /locus_tag="ACHKUN_000612"
     CDS             complement(28871..30514)
                     /gene="flgK"
                     /locus_tag="ACHKUN_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6658925.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30580..31521)
                     /gene="flgJ"
                     /locus_tag="ACHKUN_000613"
     CDS             complement(30580..31521)
                     /gene="flgJ"
                     /locus_tag="ACHKUN_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO6658926.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31521..32618)
                     /gene="flgI"
                     /locus_tag="ACHKUN_000614"
     CDS             complement(31521..32618)
                     /gene="flgI"
                     /locus_tag="ACHKUN_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6658927.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32630..33328)
                     /gene="flgH"
                     /locus_tag="ACHKUN_000615"
     CDS             complement(32630..33328)
                     /gene="flgH"
                     /locus_tag="ACHKUN_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6658928.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33381..34163)
                     /gene="flgG"
                     /locus_tag="ACHKUN_000616"
     CDS             complement(33381..34163)
                     /gene="flgG"
                     /locus_tag="ACHKUN_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6658929.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34301..35056)
                     /gene="flgF"
                     /locus_tag="ACHKUN_000617"
     CDS             complement(34301..35056)
                     /gene="flgF"
                     /locus_tag="ACHKUN_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO6658930.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35076..36281)
                     /gene="flgE"
                     /locus_tag="ACHKUN_000618"
     CDS             complement(35076..36281)
                     /gene="flgE"
                     /locus_tag="ACHKUN_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6658931.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36306..37001)
                     /gene="flgD"
                     /locus_tag="ACHKUN_000619"
     CDS             complement(36306..37001)
                     /gene="flgD"
                     /locus_tag="ACHKUN_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO6658932.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37013..37417)
                     /gene="flgC"
                     /locus_tag="ACHKUN_000620"
     CDS             complement(37013..37417)
                     /gene="flgC"
                     /locus_tag="ACHKUN_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6658933.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37421..37837)
                     /gene="flgB"
                     /locus_tag="ACHKUN_000621"
     CDS             complement(37421..37837)
                     /gene="flgB"
                     /locus_tag="ACHKUN_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO6658934.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37992..38651
                     /gene="flgA"
                     /locus_tag="ACHKUN_000622"
     CDS             37992..38651
                     /gene="flgA"
                     /locus_tag="ACHKUN_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6658935.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38727..39020
                     /gene="flgM"
                     /locus_tag="ACHKUN_000623"
     CDS             38727..39020
                     /gene="flgM"
                     /locus_tag="ACHKUN_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6658936.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39025..39441
                     /gene="flgN"
                     /locus_tag="ACHKUN_000624"
     CDS             39025..39441
                     /gene="flgN"
                     /locus_tag="ACHKUN_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO6658937.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39481..41016)
                     /gene="murJ"
                     /locus_tag="ACHKUN_000625"
     CDS             complement(39481..41016)
                     /gene="murJ"
                     /locus_tag="ACHKUN_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO6658938.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVM
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41126..42049)
                     /gene="yceM"
                     /locus_tag="ACHKUN_000626"
     CDS             complement(41126..42049)
                     /gene="yceM"
                     /locus_tag="ACHKUN_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6658939.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42051..42698)
                     /gene="yceH"
                     /locus_tag="ACHKUN_000627"
     CDS             complement(42051..42698)
                     /gene="yceH"
                     /locus_tag="ACHKUN_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO6658940.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42709..43293)
                     /gene="rimJ"
                     /locus_tag="ACHKUN_000628"
     CDS             complement(42709..43293)
                     /gene="rimJ"
                     /locus_tag="ACHKUN_000628"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6658941.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43529..44737
                     /gene="mdtH"
                     /locus_tag="ACHKUN_000629"
     CDS             43529..44737
                     /gene="mdtH"
                     /locus_tag="ACHKUN_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO6658942.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44801..45448
                     /gene="grxB"
                     /locus_tag="ACHKUN_000630"
     CDS             44801..45448
                     /gene="grxB"
                     /locus_tag="ACHKUN_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO6658943.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPPRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45582..46142
                     /gene="yceB"
                     /locus_tag="ACHKUN_000631"
     CDS             45582..46142
                     /gene="yceB"
                     /locus_tag="ACHKUN_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6658944.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46248..47294
                     /gene="pyrC"
                     /locus_tag="ACHKUN_000632"
     CDS             46248..47294
                     /gene="pyrC"
                     /locus_tag="ACHKUN_000632"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO6658945.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47368..47613
                     /gene="dinI"
                     /locus_tag="ACHKUN_000633"
     CDS             47368..47613
                     /gene="dinI"
                     /locus_tag="ACHKUN_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO6658946.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11517 a        12908 c        11853 g        11378 t
ORIGIN      
        1 tataataacc tttcattcac agtcaccagg tacaacggca tcatggcaac tgactcaaca
       61 caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg acagggatgc cgcgcttgat
      121 aaggccatga aattgttctg gcaacacggt tatgaagcga cttctcttgc ggacctcgtc
      181 gaagcgaccg gagccaaagc gcccacgcta tacgcggaat ttaccaacaa agaggggtta
      241 tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg ctaagcatga agcacagctg
      301 ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact attttgctgc catcgccaac
      361 tgctttacca gcaaagacac cccggcaggc tgcttcatga tcaacaactg caccaccctc
      421 tccccagatt caggagatat cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc
      481 actttgcagc agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac
      541 gtgacacatc tggcagaatt ccttaattgt attattcagg ggatgtcgat cagcgcacgc
      601 gaaggtgcat cgctggaaaa actaatgcag attgccggaa cgactttgcg tttatggccc
      661 gaactggtga aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt
      721 gcagagtaca gccttgttat actttgttat tcaaaatcag ttgcccatta ctatctagcg
      781 ggatctgcgt acccggatcg cggtccatgc ggattttgcc ctgctgatcg ccaatcttat
      841 aggtcacatc ataaccgagc attttttctg acttgtcata caccgtttta caacgctgtt
      901 gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg gatctggtta ccggcatatc
      961 caccacccag cgcccccaca acagtggcga catctttacc gcgaccaccg ccaaactgat
     1021 gccctatcac gccgccagca acagcgccga gcaccgaccc ggtaatgcga ttttcatcct
     1081 gcaccggtcg acgatgggtc actgtgacgt tgcgacactc ctgacgcggt gttttaaccg
     1141 tttccttgat tggggttgca gaaacaacct gagcgtattg cgggccacgt tcaaacacgt
     1201 tcagactggc cactgccgct acgcccagcg cagctgcgac accaatcccg atacccgcca
     1261 acattgattt attcacggga acacctcctt ctttgcagtt atgccaatca tgcaagcgaa
     1321 ggagaaaagc agctatcaga cagaagatga aaagcgcggg aatggctgga aatacttaat
     1381 gattcggagg agtctgaagg aatgcctgat gcgacgctta acgcgtctta tcaggcctac
     1441 cgctcgtacc atttgtccag ggcggatgag gcgtttatgc cacatccgcc agtgtacgtc
     1501 gattaatgca acttcaaacg cggacggata acgcggttaa tactccccac cagcatcatt
     1561 aatccggttt taaagtaacc atgcagcgca atctgatgca ttcggtatag cgagatatat
     1621 acaaagcgcg caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt acccatcagg
     1681 ctaccaacgg tggagaagtt cgacagcgat accagcgaac catgatcttt atactgataa
     1741 tttttcagcg gcttaccatt catctgcgcc agaatgttgt tcattgcgca agtcgccatc
     1801 tggtgtgcag cctgagcacg cggcggaaca aagccccctt ccggacgcgg gcatgacgcg
     1861 cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt gcagcgtcgg ttccaccacc
     1921 agctggttga tacggttagt ttcaagacca ccgatatctt tcaggaagtc tggcgctttg
     1981 atcccggctg cccacaccat cagatcagcc tcaatatatt cgccatcttt agtgtgcagg
     2041 ccgccttcat cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt
     2101 agctcgttgt gggccgcagc agagatacgt ggcggtaatg caggcaaaat acgttctccc
     2161 gcttctacca gcgttacgtt cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc
     2221 agttgcttga ccgcgttgtg caattcagcg gagagttcta cacccgtcgc gccgccgccg
     2281 acaatcgcaa tgttcacttt gccatttgcg cccaggttgg cggagtattt caggaagaga
     2341 ttcagcatct cctggtgaaa gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc
     2401 tctttgacac ctggcgtatt gaaatcgtta gaggtgctac ccagcgccat taccagggtg
     2461 tcataggcga ttttacgttc cggaaccagc agttcacctt tctcatcgcg cagttctgca
     2521 atagtgattg ttttcgcttc acgatcaata tcaatgacgg aacccagctg gaactggaaa
     2581 ccatgattgc gcgcatgggc cagatagctc aacgcatcga cgccttcatc aagcgagcca
     2641 gtcgccactt cgtgcagcag cggtttccac agatggctgt ggttacgatc gaccagcgta
     2701 attttggctt ttttcttgcg tcccagctta tgccccagct gtgttgccat ttccagccca
     2761 ccagcaccgc cgccgacaat cacaatcttt ttcaatggcg tagtcaacgt gaccccctta
     2821 aatttattaa ccaattgtta attaatggtt attaacatag ctcttaatta acaacaggtt
     2881 acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt ggtcatacca
     2941 aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg ggtgagatca
     3001 aaaaacagag tttattgttt attatttgag caagtccgga agggcgatat agggtgaagc
     3061 gtatatcgag ggcgtttggt cgtcctgata agaggcgcat caggcattga tgctcgaatg
     3121 tcggatgcgg cgtgaacgcc ttatccgacc cacagaatcc ggagcgcatc aggtaccggc
     3181 aaggttacct gatgcgacga gggcatttac ccgagggttt tgaacgcttt aatgcgctgt
     3241 aaatgcgggg agatattctt gaatttgtgc gtctgctctt cgtcccagac aatctcgtaa
     3301 taatgatgca gctcttcaga tgtccgctgg ctgttaagcg cttcatcatt acgcgacaaa
     3361 atcaccaggc aacgatcgcg attcttctca cggaagttgg tcacacactt agtggcaata
     3421 tcggcatact cttccgggcg atcaatcttg ccttccatgt tctcataagg gaacaaatta
     3481 gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa tccgttccgc ccagtatccg
     3541 cccagaccaa cgccgcaaat cagcggacgc tcgtcgacgt tcagttgcaa cattttgtcc
     3601 acttctttga gcagatgctg catatcatgt ttcggatgcc gcgtactgta gcttatcaag
     3661 cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc cggactgtta
     3721 gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg gttagtgacc
     3781 tgctttctct tcctgccagc gttcatgtaa ctgattcaga cgggtgctga tcgctttcca
     3841 gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca aacgtgtaac
     3901 acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt tacgattatt
     3961 gcagaccagg atcatatcgc aacccgcatc cagtgatgcc tgcccgcgtt cggcataact
     4021 gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaatcacgc cgtcaaaacc
     4081 cagttcctga cgcaaaacgg ttttcagcca gtagggagaa ccgctcgccg gacgcggatc
     4141 aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt cgcggattaa
     4201 ggaactgaag accgacatat ctttagcgcg aatctccgct tgtggacgtg gatcgcacgg
     4261 tgtttctttg tgtgagtctg ccgttactgc accgtgtcct gggaagtgtt tcccggtcgt
     4321 tttcattccg gcttcatgca taccatcaat aaaccggctg gcaattgcca gggctttttg
     4381 tggatcggca tgataagaac gctcgccaat cgccgcgctg atatgcccga catccagcac
     4441 tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca gccaacccgc
     4501 ctcttgcgcc agtttgccgc cctcttccat tcctgacagc gcagcgaatg attgcgccgc
     4561 tggcaagcgg gtaaaacctt cacgaaaacg ctgcacgcgt ccaccttcct gatctaccgc
     4621 caccaccaga tgattgcgcg aagctgcgcg gatctggcgc accagttcac gcaactgggc
     4681 aggatcatga tagttacgcg taaagagaat cagccctccc accagcggat gcgcaagtat
     4741 ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc aacattactg gacccacact
     4801 gctctcctta ttgttttatt aatagctgcc gccaggtgtc atcggccagc ctgataaatt
     4861 gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc tgctttgagc atcagcagcc
     4921 agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc gcgcgagtgg
     4981 catagtcatt gaccaattgc cggtgctgtt cagtattttc cacccacact gccgccagtt
     5041 ccagcgcgat atcaccatct ccggcatact cccagtcgat gagttttaac cctgacgcgc
     5101 tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt aaaggccgtg
     5161 gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccactgtc cgccgcgccg
     5221 gatcgctttg ctgccagtac agttccagta acggcaacag cgttattcgc cagccaaaac
     5281 gtggttgttg atgtagataa tacagcaagc ctgccagttc gttggtatcc ggcaaatacg
     5341 tttttacctc gccgggcaga tagtcgacta ccatccagtc acggagatat aaatgcggct
     5401 tcggtgcaat gcttgcgggt agttgtgata aagcccgata ctggcgcaag aacgcggact
     5461 gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctga tgttcaatga
     5521 gaaaactccc gccactaagc ccactattaa acgtcgtgac gggatgaaac tgcgggaaaa
     5581 agcgcgacag caattcgtcg cgcatgatgg gattattgct gcgaaacggc acctttacct
     5641 gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt cggagcgtta
     5701 acgttgccag aggcgctgga gtacagcacg taatgagcgc cgacattgcg ggcaatgcct
     5761 atggctttac tacgggtacc taaactgtcc tgcggcgaca aacctaactg ttgcttagcc
     5821 atcgacagct gctgggcgga aaccagggta aatttcccgt tattagccag cgcatttcgc
     5881 agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt aacgctatca
     5941 accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact gaccatcggc
     6001 tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc cgtttgatct
     6061 tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac tgtggggaca
     6121 ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac cggtgcaggt
     6181 tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa ggcgtagcga
     6241 ctcatttttg tcatcaagat tcacccctta caaatataga taaagtctga ctttgtgcgc
     6301 ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa tggtaacgct
     6361 gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt aaaaacgata
     6421 atgaacggta acgggttctt gcctttcgtt ataaagcgtt gaggatgctg aaggttgaat
     6481 atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata aagatgactc
     6541 catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac agcccaccag
     6601 cagtaacaac gccagagaca ccagcccaaa gcatcctttt ctcatcgtta cagaccttta
     6661 tgtgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat gtggtaaacc
     6721 tcttgtccgc catggcggtt ggtattcatg atcagacgat agccatcttc ggcaatacct
     6781 tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg ctcatgctca
     6841 gctgacacgt cgttcacagt cggaatgagg atattcggaa tgatcagaat atgcgttggc
     6901 gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac gatatcggag
     6961 gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac attttccttt
     7021 ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta tagagtatgg
     7081 tcgagttaaa cctgctcttt caaccttaac tcacgatttc ttaagcaaaa aatcgttttg
     7141 ctgccgtata tatcgccatt attcccattt ctgcctgcga atgcgctgaa aagacattct
     7201 ctgtttggtt tacacgtcaa atatggatgc gtatatttct catttgcatt tacatacaaa
     7261 attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc ctgacgacaa
     7321 cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag
     7381 ccagcaccaa accatcacta cttgccggtt gcatagcatt ggcactatta ccttctgccg
     7441 cttttgccgc tccagccact gaagaaacgg tgattgttga gggttcagcc acagctccag
     7501 atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa atgcagatga
     7561 ctcaacgtga tattcctcag tcggtcacta ttgttagcca gcagcggatg gaagatcagc
     7621 agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa agtcaggcgg
     7681 attccgatcg tgctctttat tattcccgcg gattccagat cgataactat atggttgatg
     7741 gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct gatatggcac
     7801 tctttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg acgggtaatc
     7861 catctgcggc aattaatatg gttcgaaaac acgcgaccag tcgtgaattt aaaggcgatg
     7921 tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta caaagcccac
     7981 tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat aacgactcat
     8041 ggctggaccg ctacaacagt gaaaagacct tcttctccgg cattgtcgat gctgatttag
     8101 gcgatcttac gacgctttca gccggttacg aatatcagca cattgatgtt aatagcccaa
     8161 cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac gatcgcgcac
     8221 gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc tttatgaccc
     8281 tgaagcagcg gtttgctgat acctggcaag cgacgctgaa tgccacccac tctgaagtcg
     8341 aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat ggtatgctga
     8401 ttgggccata cagtaattat ggacctggct ttgattatgt cggcggcacc ggttggaaca
     8461 gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtagttat gaattgtttg
     8521 gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat cgttacttca
     8581 gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt
     8641 tcccacaaac cgactggtca ccacagagcc tggcgcagga cgataccaca catattaaat
     8701 cgttatatgc tgccactcgt gtcacccttg ccgatccgct gcatctgatc ctcggcgcac
     8761 gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc
     8821 cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct
     8881 ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg
     8941 gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt
     9001 tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg
     9061 gcagcaacgg cgaaaccgcc tataaagcag tggatgggac agtcagtaaa ggggtggaat
     9121 ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctata
     9181 ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa
     9241 tgttcaccag ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact
     9301 ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag
     9361 gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac
     9421 aggggaacgt caataacctg ttcgacaaaa cctacgatac caacgtggaa ggttctatcg
     9481 tctacggcgc accgcgtaat ttcagcatta ccggcacgta tcaattctga atagcacctg
     9541 taaaaaaggc agccatctgg ctgccttagt ctccccaacg tcttacagat tagtggttac
     9601 ggatgtactc atccatctcg gttttcaggt tgtcggattt agtaccgaaa atcgcctgaa
     9661 caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca
     9721 ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa
     9781 tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgtcg
     9841 cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca
     9901 gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc gggaacagcc
     9961 acagtttgct gctgttacca gacagaacga tgaagtcgat cagaccgtgc gagaacgacg
    10021 taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt
    10081 ggatgatgta caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc tcggtgatac
    10141 cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt
    10201 ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca
    10261 ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc
    10321 cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt
    10381 tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa
    10441 acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa
    10501 tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga
    10561 tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga
    10621 aacggttaaa catgtacgct gcgatcgcac cggagataat ccctccgagt acgccagtat
    10681 ccgccaggtg tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca
    10741 cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat
    10801 cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga
    10861 cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg
    10921 cggaaccgac gcccagcaga atacctgcga taggcagtac ggataccggc agcatcagcg
    10981 atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg
    11041 agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac
    11101 agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt
    11161 tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat
    11221 atgtcacaat atttcaaacg tggttattca ccgcccaatt ttcggcggtg aactttactc
    11281 gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg
    11341 aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa
    11401 cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat
    11461 tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc
    11521 gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa
    11581 tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct
    11641 ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca
    11701 gcgtatcggc acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa
    11761 tctggatatg atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat
    11821 aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat
    11881 cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt
    11941 tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg
    12001 ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat
    12061 gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt
    12121 ctctcttaaa gatgaggaac cggtagcaca acgcccggtt gcaggtaatg ctcaatacgc
    12181 agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg taacagacat taactgttca
    12241 cgaatgtggc aaacatcccc cagtatagcc tgcaggcgcg agggagaaag atggtttgcc
    12301 agttcggcga ccaggcccgg cacatcaaca ttggtcacct gcgcagcacc atgatggcgt
    12361 tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga
    12421 ttaagggccg ctagcagcga ataccagtcg cccgatggca cgctatatgc caacgcctga
    12481 cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc
    12541 gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt
    12601 gaaagccagg tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa ccgacaacga
    12661 ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct
    12721 gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca
    12781 gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg
    12841 gtgacctcac gtaccgcatc aatgcccagc atatttttcc ctttttcggg agccagggtg
    12901 tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt
    12961 ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca
    13021 tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag
    13081 ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact
    13141 ccttaaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg
    13201 tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg
    13261 tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg
    13321 cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtcg gggcgaaaat
    13381 ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc
    13441 cctgatacgc ctgagtggag agatcgtggc gatcgccaat cacccaggtg ccgttagcca
    13501 gagctggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt
    13561 cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta
    13621 acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca
    13681 gttgctcaag cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc
    13741 cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc
    13801 acagacttgt tatggctggc aagattggta ttaaacgtgt gaccaccttt accatcggcc
    13861 acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc
    13921 ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc
    13981 gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg
    14041 gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc
    14101 tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc
    14161 tgatttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct
    14221 ttcaccattt tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt ggcggtatac
    14281 atccaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc
    14341 tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc
    14401 aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct
    14461 tctttaccgc tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg
    14521 taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc
    14581 cgtgggcgat tgatgatctt atcagcataa agctgttcac cgagcgccag acgtccggtc
    14641 cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg
    14701 cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata
    14761 atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat
    14821 ataacgttgc tgacgaaaag gagacatcgc cacaggcacg tacgggcatc actggcatta
    14881 acgcattaca aataaccatc tcatctgcct gcaacgcctc ttccagagag gcttgcactt
    14941 cgacaagctg ataagaggat tgtgccagca aacggataca gaattgtcgc ataatgccgt
    15001 taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat
    15061 tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt
    15121 ttgtctgctc aagatgagag cggatcaata cttgctcaag ccgattaaga tgtttaatac
    15181 ctgcaagatg aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt
    15241 tacgcaaacg gtcgtaatgt gcaggataaa ccgtaacgga gagaatccgc gttgccggtc
    15301 cactgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca
    15361 caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt
    15421 cacagaaaat catcaaccgc tgacaagcat cctgtagtcg ctggatatgc gccgataaca
    15481 aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg
    15541 cccgatcgct tactgccagc gattcctgct tataaccgtt aattaagaac ataccggctc
    15601 cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc
    15661 taagaaaaaa ggcccgcaag cggacctttt atatgggtgg aaaatgacaa cttagatctt
    15721 tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc
    15781 cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg
    15841 gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat
    15901 agattctact gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac
    15961 caacacacgg cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc
    16021 gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc
    16081 tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga
    16141 cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc
    16201 accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag
    16261 tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg
    16321 ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc
    16381 tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatgacc
    16441 aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc
    16501 atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg
    16561 gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag
    16621 gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc
    16681 aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc
    16741 catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc
    16801 agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg
    16861 accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc
    16921 cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccagggag ggaaaaaatg
    16981 attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg agatgttcac
    17041 ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga ttttctcagc
    17101 ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca gctcaacggt
    17161 gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa cttcttcctg
    17221 cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag tgctcatact
    17281 cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata aaatgttgaa
    17341 aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt gaggccttac
    17401 cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca gactgcgcgc
    17461 aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt tcacatgcaa
    17521 agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg cgttggcgat
    17581 ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg cacgctggtc
    17641 atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa cgtttacagt
    17701 aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca agcccgcttt
    17761 cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag aaccgatagt
    17821 gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg acagacggaa
    17881 aacagatgaa aggttggttt cgataatttc gttccactct tcatctttca ttcgcattaa
    17941 caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac caaattctgc
    18001 gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca acatcagacc
    18061 tttgccgttg gcacctaaat aatcgctgat cgcctgagcg ccattttcac tggtcgcagt
    18121 gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc caattccgcg
    18181 gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc tcttttaaag
    18241 ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca gggtgtcgac
    18301 aatgcgtttc gtcaggccag taagcacctt gcccgggccg acttcataga gatgttctac
    18361 gccttgcgct gccatgtact caacagactt cgtccactga accgggttat acaactgacg
    18421 taccagtgcg tcacggatgg catcaccatt ggtttcgcat ttcacatcaa cgttattcac
    18481 aacagaaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca gtttgtcggc
    18541 tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca gcgcgcgttt
    18601 tgcgccagca gctttacagg cagcgccagc acgctcaacc gcttctttat gaccggcaat
    18661 aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctt ctgcagcttc
    18721 ttcacacgct ttcgcaatag acgcatcatc cagaccgatg attgccgcca tagcgccagt
    18781 gccttccggt acggcttctt gcatgaactt accgcgcatc tcaaccagac gcaccgcatc
    18841 agcgaaatta atcacgccag cgcaaaccag ggcggagtat tcccccaggc tgtgaccggc
    18901 catcattgcc ggtgctttac cgccctgctg ctgccatacg cgatacagcg caacagatgc
    18961 aatcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg gcccctgctg
    19021 ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg tttcttcgac
    19081 aattggatag ctcgccgcca tatcagccag cattccaacg gtttgagaac cctgtccagg
    19141 gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa ccagcgcgga
    19201 gccccaggtg aatccaccgc caaaggcttc aagcagaacc aactgccccg gcttaatgcg
    19261 cccgtcgcgt acagcttcat ccagcgcgca cgggacagag gccgcagagg tattaccgtg
    19321 gcgatccagc gtcaccacga cattgtccat agacataccg agttttttcg ccgttgcact
    19381 gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac ggtcaagatt
    19441 atttgccgcc agcgtctcat caacgatgtg cgccagttcc gttaccgcaa ccttgaagac
    19501 ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacacggt cagcattagg
    19561 cagcgtcagc aactcaccat agctaccgtc ggcatgcaga tgggtggaga tgattcccgg
    19621 ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa taatagtccc
    19681 acgatcggtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca gagcatactt
    19741 caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga aacctgcgca
    19801 ggctgctgca acgtcaaatg ccgggcaacc tttaatgccc aacatgcttt gaatctgaca
    19861 agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca ggccaatctg
    19921 gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc ccatggttga
    19981 aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag tgacaatcca
    20041 ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt gttcgggcag
    20101 atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt tcggttatat
    20161 accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga gttccgcttt
    20221 tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga gcggcaattc
    20281 gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc gcaaaagctc
    20341 gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac agacaggcgc
    20401 cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt tgtagccaac
    20461 gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat ttcagcagag
    20521 aaaggaacat cctgacaaca ccttccatcg tctttaacgt gacatttcct gtaaagccgt
    20581 cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga tagccgatat
    20641 aattgataga agggattgtt ttaagcaccg ctgaggcatc ccgaatactg tcgagaccct
    20701 ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt tccaccacct
    20761 cttcagccag aactgagccc ataatggcaa attgcaccaa cattgtgctg tcacaatcga
    20821 cgttggcccc taaatcgagg accaccgttt tgcccttttg ctgatgtggt aataccgtca
    20881 ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc agccccatca
    20941 gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc accagctcca
    21001 gggccatacg cattgaactc ccacgactgg cgcggatagc ttgcgaaggc cgggcatcac
    21061 tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca aagtcagctt
    21121 tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg agttgcgaat
    21181 tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa tcccctccca
    21241 tgacatctaa cgccagggtt agacgtgtca aggtatcgtc gccgcccggt ttggtctttc
    21301 cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg attacttagc
    21361 gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca ggtgtttttc
    21421 accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat gggaacgacg
    21481 catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc ttactcctca
    21541 attacggttt atccccgatc caacggggag caccaattac ttacgcttta agctggctaa
    21601 tacggcaaat gggtttggct tttgcgcttc ttcaggcagt tcaccaaaga ccatgtccgc
    21661 ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg cgaggatgat
    21721 ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct caatcggttc
    21781 atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa aacaatacgt
    21841 tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg ttaccgtcac
    21901 cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg acatggagca
    21961 ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct gatcaggggt
    22021 atagataccc tggtaatcaa ggcgtttttg agccgtacga accggatcga gagtcagggg
    22081 taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag agtcaaagaa
    22141 aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca cattgcgggt tactgcttta
    22201 ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata gtttaaaatg
    22261 gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat tttagcctcc
    22321 acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga atgtgcagca
    22381 ccagaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt gcttcgactg
    22441 gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg atcatttaat tattggttca
    22501 gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga agaaaatgcc
    22561 cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg actggcgttg
    22621 tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt ccatttccgt
    22681 cacctgagcg aggcggagat tgataattac gtgcgtaagg agcatcccct gcactgcgcg
    22741 ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga gggacgtgac
    22801 cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg tcgggaaggg
    22861 aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg ttacgtcgca tccggcaatc
    22921 ggtgcaggcg gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca ttatcaggct
    22981 tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca aaccttcatc
    23041 catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact tcaacgctgc
    23101 ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc tgtcaaattc
    23161 acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg tatgcacacg
    23221 gatctgatga gtacgtccgg ttaccgggct acaacgcacc agggtggcaa atgcatagcg
    23281 ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac gcacgatacg
    23341 ttcgccgctt tgcagaatat ttttcaataa cggcgcttga acgctcttca catgcgactg
    23401 ccactgaccg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac gtaattgctc
    23461 atgcagagaa cgcaacgccg agcgtttttt cgctaccagc aacacacctg aagtgtcccg
    23521 gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc gcaaaccttc
    23581 gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag gtttattcag
    23641 caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca ccttttgcag
    23701 atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggga tacgcacctc
    23761 atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc gcacttcacc
    23821 tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg tacgcaaaaa
    23881 gttatcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta cggatggagt
    23941 ctctgttttc atggtgggcg attctaatta gccaacagga ttcgcgccac tcatttttct
    24001 atgcttatat ttagttttac accttattac ttcactacgt gatcacttta ttgatggtta
    24061 ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat aaatggtaaa
    24121 agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg tgtccatcct
    24181 tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat ccacgcagca
    24241 atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc agtccttacc
    24301 ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata attgcgctgt
    24361 ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga cgataaccgt
    24421 gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag gcgttacttt
    24481 gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa agaatgttaa
    24541 tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag cgtctgtatg
    24601 acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac aaaggtaaaa
    24661 tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct gaacgtcacg
    24721 gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac agtgctcatg
    24781 gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt cagatcgata
    24841 aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg gcgggtagct
    24901 atctggttct gatgccgaac aacccgcgcg cgggtggcat ttctcgccgt atcgaaggcg
    24961 acgaccgtac cgaattaaaa gaagcactgg cgagccttga actgccggaa ggcatggggc
    25021 ttatcgtgcg caccgctggc gtcggcaaat ctgctgaggc gctgcaatgg gatttaagct
    25081 tccgtctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg gctccgttcc
    25141 tgattcatca ggagagcaac gtaatcgttc gcgcattccg cgattactta cgtcaggaca
    25201 tcggcgaaat ccttatcgat aacccgaaag tgctcgaact ggcacgtcag catatcgctg
    25261 cattaggtcg cccggatttc agcagcaaaa tcaaactgta caccggcgag atcccgctgt
    25321 tcagccacta ccagatcgag tcgcagatcg aatccgcctt ccagcgtgaa gtccgtctgc
    25381 cgtccggtgg ttccattgtt atcgacagca ccgaagcgtt aaccgccatc gacatcaact
    25441 ccgcacgcgc gacccgcggc ggcgatatcg aagaaactgc gtttaacacc aacctcgaag
    25501 ctgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg attgttatcg
    25561 acttcatcga catgacgcct gtacgccacc agcgtgcggt agaaaaccgt ctgcgtgaag
    25621 cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt ggcctgctgg
    25681 aaatgtcccg tcagcgcctg agcccatcac tgggtgaatc cagtcatcac gtttgtccgc
    25741 gttgttctgg tactggcacc gtgcgtgaca acgaatcgct gtcgctctct attctgcgtc
    25801 tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt gttcctgtgc
    25861 caatcgcttc ttacctgctg aatgaaaaac gttctgcggt aaatgccatt gaaactcgtc
    25921 aggacggtgt gcgctgtgta attgtgccaa acgatcagat ggaaaccccg cactaccacg
    25981 tgctgcgcgt gcgtaaaggg gaagaaaccc caaccttaag ctacatgctg ccgaagctgc
    26041 atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag cgtccggaac
    26101 aacctgcgct ggcaaccttt gccatgccgg atgtgccgcc agcgccaacg ccagctgaac
    26161 ctgccgcgcc tgttgtagcc ccagcaccga aagctgcacc ggcaacacca gcaactcctg
    26221 cacaacctgg gctgttgagc cgcttcttcg gcgcactgaa agcgctgttc agcggtagtg
    26281 aagaaaccaa accgaccgag caaccagcac cgaaagcaga agcgaaaccg gaacgtcaac
    26341 aggatcgtcg caagcctcgt cagaacaacc gccgtgaccg taatgagcgc cgcgacaccc
    26401 gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat cgtcgccagg
    26461 cacagcagca gactgccgag acgcgtgaga gccgtcagca agctgaggta acggaaaaag
    26521 cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc cgtaacgatg
    26581 ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa tctgttcagg
    26641 aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag cgtcagctca
    26701 atcagaaagt gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca ccggtggttg
    26761 aagaaactgc cgctgccgaa ccaattgttc aggaagctcc agctccacgc acagaactgg
    26821 tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa gagaacaatg
    26881 ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct cgtcacctgc
    26941 gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc cagtcgccaa
    27001 tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc tggatccgct
    27061 atccaattgt acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag gaagaagtac
    27121 aagtgcagcc gatggtgact gaggtccctg tcgccgccgc tgtcgaacct gttgttagcg
    27181 cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc cgtaccggtt gccgaaccgc
    27241 aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcggta actgaacagc
    27301 cgcaggtgat taccgagtct gatgttgccg tagcccagga agtcgcagaa cacgcagaac
    27361 cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa actgctgagg
    27421 ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg
    27481 cagcagaagt tgaaacggta gccgcggttg aaccagagat caccgttgag cataaccacg
    27541 ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt tccggaggca ccgcgtcaca
    27601 gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg
    27661 caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa taattagctc
    27721 aaagtaatca agccctggta actgccaggg cttttttatt tcatctttga atcaacatat
    27781 taaaattcat caaatggcat ccttgctaac caacaatgca aaataggcaa aaaaatgccc
    27841 gccccggcga tcccagagca ggcagacaaa acatacccag tttcatgata tgttttaaag
    27901 cgagcttatt tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac
    27961 gatgcctgca atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca
    28021 tcaaccagat cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taatgaatcc
    28081 agcgactcca gttcgttcag ctgcgtgcct aattccgcgc gaactgtaag gacattattc
    28141 agcgagtttt tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc
    28201 gcttcgctat ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa
    28261 agattggttt cagaagcgct accgtctggt tccgctaccg cgttactggt aatactgtcg
    28321 aaaattttgt cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac ctgttgccta
    28381 atactttctg ctccaccgac gtatttccct ttctcttcgc taaacggcgc agtctctgtt
    28441 ttataaccgg caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac
    28501 tggtcacgaa gcccctggat atccgtagcc agcgaggccc ggtcatcgtc actcaaagtg
    28561 ccattgctgg cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta
    28621 agtacactct cttccagtga cactttttga gtggcgaaag tacgcgccag cgtgtactgg
    28681 ctgttttgcg cctgtgcctg ggagagaaca accgcttgtg atgctgcaat gggatcgtca
    28741 gaagggttaa cgactcgctt acctgtcgac atctgctcgc cgtacttcat ccattccgcc
    28801 tgagaattgg tgatgccacg catgttttgc tggtacatca tctgggtact gaaacgcatc
    28861 ttatctcccc ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa
    28921 cctgcgcatt cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcctcatcga
    28981 gattgacacc ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg
    29041 tggcgctact ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat
    29101 aagcgtcgtt aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca
    29161 gggcctgacc gttgcggtta tcgctatcac ccgcatcttc ttcgctcgcc atcgctattt
    29221 tcgcttcgtc ggtgattaat acatccatgt tgacgatagc gtcacttact ggtttcagcg
    29281 tgaagctgtc gttaacagca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca
    29341 ctttaccgtt ggcatccggc gtcaccgtaa aagtggtatt actggcaagg cgggtgacct
    29401 gccactgatt attatcgaac gagattttgt aatcagtcgc cagtaccgcg gaggcatcag
    29461 ttaccgtggc accgatcgca acgtcacctg tgtttttcgt gttttgcaga accgcgggct
    29521 taccgatagc aaagaaatct tcaccagcat cgccgttggc atcaaatccg gctttgtgtt
    29581 ggctgttgaa agcctcggca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt
    29641 ccagatcctg agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct
    29701 ccgggatctc aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt
    29761 cggcgctgga aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac
    29821 cattggccat cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa
    29881 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    29941 ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt
    30001 aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    30061 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    30121 cctggcgcgc tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    30181 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    30241 tctgctcata gcgggcagtc aggccgctac tttgcgtctg cgccgcacgt aactggttgg
    30301 taataaaagc atcatactca cgctgcacac cagaaacgta gacaccattg ccaacccagc
    30361 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    30421 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    30481 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    30541 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    30601 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt
    30661 gagtttacgg gcatagtgag gatcggtagc ataccccgcg tcctgtaggg cctgcgcccc
    30721 ctgctccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc
    30781 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt
    30841 cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtaactggcc ctttccagtt
    30901 gccagaggct ttgacaccaa acaggttata gctcggctcg ccgttttcgc ggcggatttg
    30961 ccgttgcccc caaccagatt ccagtgccgc ctgagcgagg atcaaatgat gtggcacacc
    31021 gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctttactgtc
    31081 acccggcagc gaatcatcgt agttacgtgg cacggccttt tgcaccagct gcgaaagcgc
    31141 ctgattttga taacgcacca cagtttcgag cgggaatttc atcggtgctg ctggcgtgga
    31201 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag
    31261 acctttgccc gccgtcatct gttgggcaat ctgctggtca tacatactgg tatacaggcg
    31321 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    31381 catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc
    31441 ttcgcccgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    31501 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    31561 tgcattgatt gcagtatgga catcagatcc atcggcgtag cgcccagcgc attgagcgcg
    31621 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    31681 cgtaaatcga tctgcgtttg tggagtaacc acagtctgtc caccaccaaa cggtgtatct
    31741 ggctggctga cattggcctg acgattgact gttactgaga gatttccctg cgctaccgcg
    31801 cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact
    31861 actttagcgt cctgcggggt gacattaacc tgcatattct ggatatctgc aaggaaacgg
    31921 acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg
    31981 gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa
    32041 tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca
    32101 cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttcac ctgaacactg
    32161 ctaccgccag cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac
    32221 acctgactgt caacgccctt aagcggcgtc atcaacaacg tacctccacg caagcttttg
    32281 gcatttccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga
    32341 agtgacgctg tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga
    32401 acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg
    32461 gttgtctggt caccggtgcc atccagcccc accaccagac catagccaat cagtgagttt
    32521 tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta
    32581 accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg
    32641 acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat
    32701 tgcctacgta ttcaatgcgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc
    32761 tgatagtgcg tggattaacc acgccagaga agcgaataaa ttcggtaccc tgattaatgg
    32821 caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca
    32881 ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt
    32941 taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg
    33001 gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt
    33061 tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc
    33121 gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag
    33181 aaccgttggc gacgggcgtc ggcccgggaa ccggttgtgc actggtcgcc ccctgcacca
    33241 gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg
    33301 cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg
    33361 aacctgccac cggttaagcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg
    33421 gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct
    33481 tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc
    33541 aggcccggcg tgctttcgtt cggtgcacca gaggattgcg tttcggtgta gaggttttcg
    33601 ccaatgctct ccagcccggt gtcattcata aaggtggtga gattgagctg cccaacctga
    33661 accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata
    33721 cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc
    33781 agctgcccgt tctgatccac ctggaaagag ccgtcacggg tataggctga tgaaccatct
    33841 ggcaacatca cctggaaaaa gccctgccct ttaatcgcga catctttgct gttgttggtc
    33901 tgcgacaggt ttccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg
    33961 atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg
    34021 gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg
    34081 tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg
    34141 gcgatccata atgaactgat cataaaatgt cctgtagtgg attagtgaca agcagagcag
    34201 aagcacgcga cagtgtgaat ttcccctcac cctaaccctc tccccaaagg ggcgagggaa
    34261 tagtccgcgc gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt
    34321 ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac
    34381 gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca
    34441 gaacccccga catcacacgc aaggttggat ctgcctgcag tatcggccca cgcgtggcct
    34501 ggctttctgc gcttaaacga aaaatgccgt cgtcaccgcg ttgcacttcg ctgcccgtgg
    34561 ctttcaccag tttcagacgc cccactggcg caaccgtatt tgccggatcg cccggattga
    34621 gcgccgagat tgtgccatcg gcagcaatag tgatttccgc cccttccggc acagcaattg
    34681 gcccagcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg ggatcaacct
    34741 gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggactgcacg gccagccagc
    34801 catcctgctg caatgcaacg tccagcgggc gcgaggtgta atccatcttg ccgggcgtca
    34861 tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca
    34921 ccggcactgc acgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca
    34981 gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcggtat
    35041 aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac cagcgtgttg
    35101 aggatctggt cctgggtttt gatggtctgg gcgttagact gatagttacg ctgggcaacg
    35161 atcatattga ccagttcttt actgagatcg acgttggacg cttccaacgc accgttggtc
    35221 agggtgccaa agttgcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc
    35281 gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt
    35341 acaatctgcc ccagcagttg tgtttgttcg ttggaatagt tgccgacaac cgtaccgtca
    35401 tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt ggttgccaca
    35461 atattgttag cgccggtatt ttgctgcatg gagttgagga agctcaggct aaacgtggcg
    35521 ggatctgcgc cgttaattgc gccagtggta atattctctg ttggatttga ggtcagaacg
    35581 ccattggcat taaacaccag cttcattgca ggctcggctg tacctgttgg atcactgcta
    35641 tcctgggtgt agacctgcca gttattatcc ccggtcttca caaagtagac gctcatgtca
    35701 tgagcattac cctgactgtc gaaaacagtc accgaacctt ttttgttata gctatccgca
    35761 ttgctggcgc tgaatggcgt aacagtagga agcggatcac tggaattcag gttgatctgc
    35821 atcgatgccg tggtggtagt tttcgctgcc atcagggtat tcgggatcga aatattggtc
    35881 ggattcgccc cttgctgaat agtcggcggc gtaccggttg ccgggtaacc cgtcagctgt
    35941 aaaccttgca tattcaccag gttacggttt tcatccagct taaattgtcc gttacggctg
    36001 tagaacaccg aaccgttgct gtctaccaga cggaaaaaac cgttctggct gatagcaacg
    36061 tccagacctc gcccggtgtt ggtggtcgtg ccatcggtaa agtcctgagt gataccggca
    36121 acttttaccc ccagtcccac tttcgaaccg gcaaacatat cggcaaaaga ggccgtgcct
    36181 gatttaaagc cgtaggtggc ggagttggcg atattgttgc caataacatc gaggttggtg
    36241 gcggcagcgt ttaatccgct aaccgcttga gaaaaggcca tgactgactc ctgaaatgtg
    36301 aaggcttaaa ttatctgccg tacttcgtcg agggtggtgg tgccgtaagt gccgagatcc
    36361 agcgtattac cgctgttgcc gcggatcaca ccctgcacca gagcaaactg cagcggctgg
    36421 gcaaccagtt gtgtaccacc gttactggcg ctaatcgcta cattgtaaga accgttcggc
    36481 gcagtgctgc cgtcagtcaa cgtaccgtcc caggtgaaac tgtgaactcc agcggtcagt
    36541 tcaccaatat caatggtgcg cacaaccgca ccatttttat cggtgatggt ggccgtaact
    36601 ttgtctgccg cctgttgcag ctcaacacca aacggcgtgg tcgtggtcac agctccttct
    36661 tcactgccgg ttcccgcaag aacagtggtg ccggggatca tcacgccgtg accgatcagg
    36721 ttactggcct gtaacgactg gctgttatca atctgtccgg aaatagatcc gagtgtggta
    36781 ttgagttttt caatcccgct gaccgtgctg atttgtgcca attgcgacgt cagctcgttg
    36841 ttttccattg gattggtcgg gtcctggttt ttcagctgcg ccaccagcaa agtcagaaaa
    36901 ctgctttgta aatctgcggc gttgctgccc gtgagcgaac tactgctggt ggtactgacg
    36961 ccggtatttg tcggatcggt ggtggttacc gcaatggaca tagctttctc ctttattgac
    37021 cgagcgtaag ggttttcagc atcatgcttt tcaccgtgtt gagcacttca acgttggcct
    37081 gatagctgcg tgacgccgac atggtgttaa ccatctcccc gacgacatca acgttcggca
    37141 tttttacgta gccctttgca tctgccagcg gattacccgg ttcataaacc agtttgtccg
    37201 gggcctgact ttctataaca tcggcaacct ttacgccgcc tgtcgcagca cctggtgcag
    37261 cgttaacctg gaataccacc tgttttgccc gatatggctg tccatcagga ccggtcacgc
    37321 tatcagcatt tgccagatta ctggccgcca cgttcaggcg ctgggactgg gcagttaacg
    37381 ccgacccggc gatatcaaaa atattcagca gtgccatccg ttaatttccg ctctgtaaaa
    37441 cgttcatcat gcctttgatt tgcccgctca acgcgctaag gctcatctgg tattgcaggc
    37501 tgttatcggc aaactgggtg cgttcgcgat ccatatcgac ggtattaccg tcaagcgaag
    37561 gctggtccgg aatacggtat tgcagttctg cggtaggagg cgtcagcgcc tgcgccggga
    37621 tgtgttgcgt tgaggtcatc gtcagtgcaa ccacactggt tgcatcccgt ccacgttgca
    37681 tgactttttt aagttcactg gcaaaatcga tatcgcgcgc ctgataacca ggggtatcgg
    37741 cattggcgat gtttgctgcc agcacttcct gacgctgggc gcgcagattg agcgcctctt
    37801 gttgaaaacg taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat
    37861 tctgccatca gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg
    37921 tcgctattta tgccgttgat ggtcattgcg gacaggtaca attcacgttg tagaaatggc
    37981 tgggggcgaa aatgctgaca ataaaacgta gcgtggcgat catcgcgata ctgttcagtc
    38041 cgttaagtac ggcgagcaat ctcacatcgc aattgcacaa cttttttagc gcccaactcg
    38101 cgggggtaag tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat
    38161 gcgagcagcc attgctttcg atgagcaata attcccgcct gtggggcaat gtgaatgtgt
    38221 tggcacgctg cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt
    38281 atgtggttgc cgcgatgccc attgtgcggg gaggaaagct ggaagctggc aatgtcaaac
    38341 taaaacgcgg acggctggat accctgccac cgcgtacggt gctggatatc aatcaacttg
    38401 ttgatgccat tagcctgcgc gatctatcac ccgatcaacc tatccagtta acccagtttc
    38461 gccaggcatg gcgggtaaaa gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt
    38521 ttagcgccaa cgcagaaggt caggcgctga acaatgcagc cgtcgcacag aatgcgcggg
    38581 tgcgcatggt atcgggacag gtagtcagcg gcgttgttga tgcagatggg aatattctta
    38641 taaacctgta agctgttaaa gattacccgt cccttgccga taaataagca acacatgata
    38701 aaagcgccct caatgaggaa taaaccatga gtattgatcg cacttcgcct ctgaagcctg
    38761 taagcaccgt tcaaccgcgc gaaaccactg acgcgccggt aacgaacagc cgggcggcaa
    38821 aaacaaccgc ctccaccagc accagtgtga cgttaagcga cgcgcaagca aaactgatgc
    38881 aacccggcag cagtgatatc aatcttgaac gtgtcgaagc gttaaaactg gcgattcgta
    38941 acggtgaact aaaaatggac accggcaaaa ttgccgatgc gctgatcaac gaagcgcagc
    39001 aagacttgca gagtaactga tcgtatgaca cgtcttgcag agatcctcga ccagatgtcc
    39061 gctgtgctta acgatctcaa aacggtaatg gatcaagagc agcaacatct ctctatgggg
    39121 cagatcaacg gcagccagtt gcaatggatt acagaacaaa aaagctcact gctggcgacg
    39181 ctggattacc tcgaacagtt acgcaagaaa gaacccaata cagcaaatag cgttgatatt
    39241 agtcaacgct ggcaggaaat tactgtgaaa acgcagcaac tacgccaaat gaatcaacat
    39301 aacggctggt tactggaagg acagattgag cgcaatcaac aggcgctgga aatgttgaaa
    39361 ccgcatcagg aaccgacgct atatggggcg aacggtcaga cctcaacaac ccatcgcggc
    39421 ggtaaaaaga tttcgatctg aatattatct ccggcctgca ctgcaggccg gaatgcattg
    39481 ttacaccgtc cggcgggcaa attctttaac tttgaagccc agtaccgcca gtgcagcgaa
    39541 gtacgcggca atccccgcca gcacgaccgc cattaaacgc agtaagcgcc agggcatggt
    39601 acccaatgac cactccggca tgatatgtaa catacctaaa agcacgccag acatcaccag
    39661 taccgccatc accagacgca acagaaacgc catccagccg ggttgcggag taaagatttt
    39721 ctgcttacgc aactgccagt aaagcagcga agcattcaga cacgccgcca gaccaataga
    39781 aagtgacagc ccggcatgtt tcaacggacc aataaacgcc aggttcatca attgcgttaa
    39841 aatcagcgta acgatggcaa ttttcactgg cgttttaatg tcctggcggg aataaaagcc
    39901 aggagccaac actttcacta caatcaggcc aatcaaaccc accgagtagg caattaacgc
    39961 ccgctgggtc atcagcgcat caaacgcggt aaatttaccg tactggaaca gcgaaacggt
    40021 taacggacca gaaagaatgc ccaacgcaac cgcactcggc agcgccaaca ggaaacaaag
    40081 acgcaacccc cagtccatca aacggttgta ttcatcatga ttgccactgg caaaactttt
    40141 cgacagcgac ggcagcaaaa tggtgccaag cgccacgccc agcacaccgg acggaaactc
    40201 cattaagcgg tcggcgtaat acatccaaga caccgaaccg gaagcaagaa acgaggcaaa
    40261 aatggtgttg atgattaagg agatctggct cacagagacg ccaaggatcg ccggtcccat
    40321 ctgtttcacc acgcgcattg ttccggcatc gtggaagtta atgcgcggca ggaccagcat
    40381 gccgatcttc ttcaggtgcg gtagctgata caccagctgc aggatgccgc cgaccgtaac
    40441 agcccacgcc agcgccagca ccggtgggtt aaagtacggt gcggcaaaca gcgcgaaacc
    40501 aatcatgctg atgttaagca gtgttggagc aaacgccgga atcgagaagc ggttccacgt
    40561 attcagaatc gctcccacca gcgacgccag ggagatcagc aagatatagg gaaaggtaat
    40621 ctttagtagc tggctggtca gggcaaattt gtcagctgtg tcagcgaagc ctggcgcggt
    40681 caccatgatc acccacggtg cggcgagcat gccagcgacc gtcacaaccg ccagcgcgag
    40741 tgtcagcagg ccagaaacat aagagacaaa gacccgcgtg gcgtcttcac cctgcttact
    40801 tttatattcc gccagaatcg gtacaaatgc ctgggaaaat gccccttcgg caaagatacg
    40861 gcgtaacaag ttaggaagtt taaaagcgac gaaaaaggcg tcggttgcca tccctgcgcc
    40921 aaagattctg gcgacaattg cgtctcgtgc gaagccaagc acacgcgaaa acatggtcat
    40981 cgagctgacg gcggccagcg attttaataa attcatcggt gttctaatcc agacccacgg
    41041 gcaaaacgcc tgcaaggcag gcgctgaaag aggcgaatag tctacctgga ttatggtgaa
    41101 ttgctaccgc cagatgttac agggtttatt cactcatcgc atcgcgccag atcttgtcaa
    41161 cgatacgttg cgccagcacg gcctgttcgc cggcggtttg cggaactgtc tgattttgca
    41221 cacattcaat gaagtgccgc gcacagccga caaatccacg ttgttcaaga gtgctctgcc
    41281 aaccaggaat cggtttatgc actacgccct gcccgcgctc ctcacgccat tcgcgcatat
    41341 ccgtaatgtc gatgagcgca ccgtcagtca cggcctgcac ggtttcacgc tgacttccgg
    41401 cacggcgatg catacaggtg gtgatctgca aaggaccggc cgaaaaatgg tgctcggcaa
    41461 acagcatttc gccagcgtcg ttagtcagta gcgtaccgcc atccagagag gctttgccgc
    41521 ccgacaacca cagcacggta tccaccacat gcagataatc atccagcaac gtgaaataaa
    41581 gatcgtgtgg cccgacacta ttgctacgat gtttatccat tcttagcgaa gctgcggtag
    41641 cgagttgcgt ttttaactca ccgtagagtg gtgcgaaacg acggttaaaa ccgaccatca
    41701 acgtcagttt tttacgcgcc gccagttcca ccagccgttc agcatcgcgc agattttctg
    41761 ccagcggttt atcgacacag acatgtacac ccgcattgag taacgtactg accacgtcaa
    41821 agtggctggc ggtgctggaa tgcacaaaaa ccgcatcgca actggcggca aggctggata
    41881 acgaatcggc ataaggaatg cgccagcttt cacaaattgg cagggctttc gcgcgcgtag
    41941 gcgaccaggc tccttgtaac gtccagtcag acgctgccgc cagcaccggt aaccacgctt
    42001 tttgcgcaat gccacctaat cccactacgc cgatacgtaa ttttttcact ttaatctccc
    42061 agatgggcca gcaacgaatc aagacgctgt ttcagttctg ccacttcgat ttccagggct
    42121 tcgacgcggg cctgtaaatc accatcaacc gcgttcgaca tatccgtcac cgccggctga
    42181 tcttcaacct caccactgaa aagatgcatg tagcggcttt cgcgtttacc cggttcgcgg
    42241 gccagacgca ccacaaaagg accatcttcg cgatttgcca gttgttccag cgtcgactcc
    42301 acttccgcca tatcgctgaa ttcatacatt cgcgcggcgc ggctgcgcag ttcacctggc
    42361 gtctgggcac cacgcaataa caacgtggtg attaacgcca cttccgctgc gctcagtttc
    42421 agatcgccaa attctgaatt acaaaaacgt tgctcatatt tggtgacccg attaccaaaa
    42481 ccgctcactg tgcgtagata atgacgtttg accagattat ccagctgttc ctgcacttcg
    42541 gattcactca gattcatgac cggttcacgg ttcgttttct gattacaggc cgtgactaca
    42601 ccattgactg agagcgggta ttgctccggc gtggtcacct gcttttccag caagcaacca
    42661 atcacgcgag cttcaagggc agtaagttga tatttcatga gattctcctt agcggccggg
    42721 cgtccagtct ggggtagtta atgccgtcag tacgtgatcg cgccattgtc catcaatcaa
    42781 cagatagtct ttcgcatagc cttctttttc aaaacccagt cgcgccagta aatcaccgct
    42841 gcgtttattg tgcggcatat aattagccat aatgcgatga atatgttggg tgcgctgcat
    42901 ataacgaatg gctgcggtca gggcttcaaa catgagtcct ttgccctgcc atttttgccc
    42961 aatcgaataa ccgagatagc aggcatgaaa agagccacga acaacattgg aaaaattggc
    43021 aacgccaata atctcttttt cgtccgggtc gaataagcca aagtagaaag ctgaaccttg
    43081 tttatgaaat tcgttaatca tccccagcct ggcctgccag cctgatggat aacagtggct
    43141 ttcgtcgcgc actggctccc agggcttgag gaaatggcga ttctctgcgt aataatccgc
    43201 aagacgccag gcatcacgat catgcaccag acgcacgacc agtcggtctg tggttaagcg
    43261 cacttttggc acgttactgc gatagccaaa cattctatac ctactccttc ccgtaacgtc
    43321 tttaatacgc tgtaagtatt actatacctg cgcaaccaga ccctgtgaaa agtccaaaat
    43381 gccattttct gcattattca catatttgat aaaaactctc tatcaaagcg acgttttgat
    43441 aaaaaaatat tgtccccgaa gtgatgggaa aagtcttagc ggcaatgcag aatagaaagt
    43501 gctcatcttt tatttcccgg gaggggaaat gtcccgcgta tcgcaggcga ggaacctggg
    43561 taaatatttc ctgctcatcg ataatatgct ggtcgtgctg gggttctttg ttgtcttccc
    43621 gctgatctct atccgcttcg ttgatcaaat gggctgggcc gccgtcatgg tcggtattgc
    43681 tctcggtcta cgccagttta ttcagcaagg tctgggtatt ttcggcggcg caattgccga
    43741 ccgctttggt gccaaaccga tgattgttac cggtatgctg atgcgcgccg ccggattcgc
    43801 cacaatgggt atcgcccacg aaccgtggct attgtggttt tcatgcctgc tctcgggact
    43861 cggcggcacg ttgtttgatc cgccgcgttc ggcgctggtg gtgaaactaa tccgtccaca
    43921 acaacgtggt cgttttttct cgctgttgat gatgcaggac agtgccggtg cggtcattgg
    43981 cgcattgttg gggagctggc tgttgcaata cgactttcgc ctggtctgcg ccacaggggc
    44041 cgttttgttt gtgctgtgtg cggcgttcaa tgcgtggttg ttaccggcat ggaaactctc
    44101 caccgtacgc acgcccgttc gcgaaggcat gacccgcgtg atgcgtgaca agcgttttgt
    44161 cacctatgtc ctgacgctgg cgggttacta catgctggct gtacaagtga tgctgatgct
    44221 gccaattatg gtcaacgacg tggctggcgc accctctgcc gttaaatgga tgtatgccat
    44281 tgaagcgtgt ctgtcgttaa cgttgctcta ccctatcgcc cgctggagtg aaaagcattt
    44341 tcgtctggaa caccggttga tggctgggct gttgataatg tcattaagca tgatgccggt
    44401 gggcatggtc agcggcctgc aacaactttt caccctgatt tgtctgtttt atatcgggtc
    44461 gatcattgcc gagcctgcgc gtgaaacctt aagtgcttcg ctggcggacg caagagctcg
    44521 cggcagctat atggggttta gccgtctggg tctggcgatt ggcggcgcta ttggttatat
    44581 cggtggtggc tggctgtttg acctgggcaa atcggcgcac cagccagagc ttccgtggat
    44641 gatgctgggc attattggca tcttcacttt ccttgcgctg ggttggcagt ttagccagaa
    44701 acgcgccgcg cgtcgtttgc ttgaacgcga cgcctgattt gatactcccc cctgactcct
    44761 ccatactgaa agtgaactga cagccaaatg gaggagtcac gtgaagctat acatttacga
    44821 tcactgccct tactgcctca aagcccgcat gattttcggc ctgaaaaata tccccgtcga
    44881 attacatgtt ctgctcaacg acgacgcaga aacacccccc cggatggtcg gtcaaaaaca
    44941 ggttcccatt ctgcaaaaag atgacagccg ctatatgcca gaaagcatgg acatcgttca
    45001 ctatgtcgat aaactcgacg gcaaaccgtt actgaccggc aaacgttccc ctgccattga
    45061 agagtggctg cgcaaggtca atggctacgc aaacaaactg ctgttgccgc gttttgccaa
    45121 atcggcattt gatgagtttt ctactcccgc cgcgcgcaaa tatttcgtcg acaagaaaga
    45181 ggccagcgcg ggtaatttag ccgacctgct ggcccactct gacggtctga ttaagaatat
    45241 cagcgatgat ttgcgtgcgc tggacaaact gatcgtcaaa ccgaacgccg tgaatggcga
    45301 actttcggaa gatgatattc agctatttcc gctactgcgt aatctgacgc tggtagccgg
    45361 aatcaactgg ccaagccgcg ttgctgatta ccgcgataat atggcgaaac agacacaaat
    45421 caatttgtta tcatcaatgg cgatttaatc ctgacccgcc gcggcagcct gtattcgcgg
    45481 gttgccgccg ttttcagttt tttctgctgg cgagtttatc gctggtgaca catgctgcgt
    45541 tgtgtcagga tattgacgca gatttcatgt cgaggactac catgaacaag tttttatttg
    45601 ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa tcaactcacc caatacacca
    45661 tcaccgaaca agaaattaac cagtcgcttg cgaaacataa taatttctca aaagatatcg
    45721 gtttacccgg cgtggctgac gcccatattg ttctgacaaa cttgaccagc caaattggtc
    45781 gcgaagagcc gaataaggtt accctaaccg gagacgccaa tctggacatg aactccctgt
    45841 tcggtagtca gaaagcgacc atgaaactga agctgaaagc gctgcccgtg tttgataaag
    45901 aaaaaggtgc gatcttcctg aaagagatgg aagtggtcga tgcgacggta caaccggaaa
    45961 aaatgcaaac ggtgatgcaa acgttgcttc cctatttgaa ccaggcatta cgcaattact
    46021 ttaaccagca acctgcttac gtcctgcgcg aagatggcag tcagggcgaa gcaatggcga
    46081 aaaaactggc gaaaggcatt gaagtgaagc caggcgaaat tgtcattccg tttacggatt
    46141 aatgacgagg gcgcattcgc gccctttatt tttcgtgcaa aggaaaacgt ttccgcttat
    46201 cctttgtgtc cggcaaaaac atcccttcag ccggagcata gagattaatg actgcaccat
    46261 cccaggtatt aaagatccgc cgcccagacg actggcacct tcacctccgc gatggcgaca
    46321 tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg acgggctatt gtaatgccca
    46381 atctggctcc gcccgtgacc actgttgagg ctgccgtggc gtatcgccag cgtattcttg
    46441 atgccgtacc tgccgggcac aatttcaccc cattgatgac ctgttattta acagattcgc
    46501 tggatcctaa tgagctggag cgcggattta acgaaggcgt gttcaccgct gcaaaacttt
    46561 atccggcaaa cgcaaccact aactccagcc acggcgtgac gtcaattgac gcaatcatgc
    46621 cggtacttga gcgcatggaa aaaatcggta tgccgctact ggtgcatggt gaagtgacac
    46681 atgcagatat cgacattttt gatcgtgaag cgcgctttat agaaagcgtg atggaacctc
    46741 tgcgccagcg cctgactgcg ctgaaagtcg tttttgagca catcaccacc aaagatgctg
    46801 ccgactatgt ccgtgacgga aatgaacggc tggctgccac catcactccg cagcatctga
    46861 tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc gcacctgtat tgtttaccca
    46921 tcctcaaacg caatattcac caacaggcat tgcgtgaact ggtcgccagc ggttttaatc
    46981 gagtattcct cggtacggat tctgcgccac atgcacgtca tcgcaaagag agcagttgcg
    47041 gctgcgcggg ctgcttcaac gccccaaccg cgctgggcag ttacgctacc gtctttgaag
    47101 agatgaatgc tctgcagcac tttgaagcat tctgttctgt aaacggcccg cagttctatg
    47161 gcttgccggt caacgacaca ttcatcgaac tggtacgtga agagcaacag gttgctgaaa
    47221 gcatcgcact gactgatgac accctggtgc cattcctcgc cggggaaacg gtacgctggt
    47281 ccgttaaaca ataaaaatcg tagcttcctg ttgtcattag gttattttac ctgtataaat
    47341 aaccagtata ttcaacaggg ggctattatg cgaattgaag tcaccatagc gaaaacttct
    47401 ccattgccag ctggggctat tgacgccctg gctggcgaac tttcccgccg tattcagtat
    47461 gcgtttcctg ataatgaagg ccacgtatcg gtacgttatg ccgcagcgaa taatttatcg
    47521 gttattggcg caacaaaaga agataaacag cgcattagcg aaattctcca ggaaacgtgg
    47581 gaaagcgccg atgactggtt tgtcagcgaa taatatgcag tgatttttgt ttttgccggg
    47641 tcgccccggc tttttt
//
LOCUS       DAZFRJ010000012        45458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000012 DAZFRJ010000000
VERSION     DAZFRJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.012"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(92..622)
                     /gene="cybB"
                     /locus_tag="ACHKUN_000634"
     CDS             complement(92..622)
                     /gene="cybB"
                     /locus_tag="ACHKUN_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO6658947.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            811..1812
                     /gene="gap"
                     /locus_tag="ACHKUN_000635"
     CDS             811..1812
                     /gene="gap"
                     /locus_tag="ACHKUN_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6658948.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1854..3293)
                     /gene="aldA"
                     /locus_tag="ACHKUN_000636"
     CDS             complement(1854..3293)
                     /gene="aldA"
                     /locus_tag="ACHKUN_000636"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6658949.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3490..4290)
                     /gene="ydcF"
                     /locus_tag="ACHKUN_000637"
     CDS             complement(3490..4290)
                     /gene="ydcF"
                     /locus_tag="ACHKUN_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO6658950.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(4562..8464)
                     /gene="hrpA"
                     /locus_tag="ACHKUN_000638"
     CDS             complement(4562..8464)
                     /gene="hrpA"
                     /locus_tag="ACHKUN_000638"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO6658951.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            8665..9270
                     /gene="azoR"
                     /locus_tag="ACHKUN_000639"
     CDS             8665..9270
                     /gene="azoR"
                     /locus_tag="ACHKUN_000639"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO6658952.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(9321..10637)
                     /locus_tag="ACHKUN_000640"
     CDS             complement(9321..10637)
                     /locus_tag="ACHKUN_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO6658953.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(10627..12384)
                     /locus_tag="ACHKUN_000641"
     CDS             complement(10627..12384)
                     /locus_tag="ACHKUN_000641"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO6658954.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(12400..13296)
                     /locus_tag="ACHKUN_000642"
     CDS             complement(12400..13296)
                     /locus_tag="ACHKUN_000642"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6658955.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(13296..13901)
                     /locus_tag="ACHKUN_000643"
     CDS             complement(13296..13901)
                     /locus_tag="ACHKUN_000643"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6658956.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(14072..16378)
                     /locus_tag="ACHKUN_000644"
     CDS             complement(14072..16378)
                     /locus_tag="ACHKUN_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO6658957.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNNTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(16442..17302)
                     /gene="pdxI"
                     /locus_tag="ACHKUN_000645"
     CDS             complement(16442..17302)
                     /gene="pdxI"
                     /locus_tag="ACHKUN_000645"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO6658958.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(17533..18123)
                     /gene="paaY"
                     /locus_tag="ACHKUN_000646"
     CDS             complement(17533..18123)
                     /gene="paaY"
                     /locus_tag="ACHKUN_000646"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO6658959.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(18105..19055)
                     /gene="paaX"
                     /locus_tag="ACHKUN_000647"
     CDS             complement(18105..19055)
                     /gene="paaX"
                     /locus_tag="ACHKUN_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO6658960.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(19156..20469)
                     /gene="paaK"
                     /locus_tag="ACHKUN_000648"
     CDS             complement(19156..20469)
                     /gene="paaK"
                     /locus_tag="ACHKUN_000648"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO6658961.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGGAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(20496..21701)
                     /gene="paaJ"
                     /locus_tag="ACHKUN_000649"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(20496..21701)
                     /gene="paaJ"
                     /locus_tag="ACHKUN_000649"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO6658962.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(21701..22123)
                     /gene="paaI"
                     /locus_tag="ACHKUN_000650"
     CDS             complement(21701..22123)
                     /gene="paaI"
                     /locus_tag="ACHKUN_000650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO6658963.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(22113..23540)
                     /gene="paaH"
                     /locus_tag="ACHKUN_000651"
     CDS             complement(22113..23540)
                     /gene="paaH"
                     /locus_tag="ACHKUN_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO6658964.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(23542..24330)
                     /gene="paaG"
                     /locus_tag="ACHKUN_000652"
     CDS             complement(23542..24330)
                     /gene="paaG"
                     /locus_tag="ACHKUN_000652"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO6658965.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(24330..25097)
                     /gene="paaF"
                     /locus_tag="ACHKUN_000653"
     CDS             complement(24330..25097)
                     /gene="paaF"
                     /locus_tag="ACHKUN_000653"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO6658966.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(25094..26164)
                     /gene="paaE"
                     /locus_tag="ACHKUN_000654"
     CDS             complement(25094..26164)
                     /gene="paaE"
                     /locus_tag="ACHKUN_000654"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO6658967.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(26172..26669)
                     /gene="paaD"
                     /locus_tag="ACHKUN_000655"
     CDS             complement(26172..26669)
                     /gene="paaD"
                     /locus_tag="ACHKUN_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO6658968.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(26684..27430)
                     /gene="paaC"
                     /locus_tag="ACHKUN_000656"
     CDS             complement(26684..27430)
                     /gene="paaC"
                     /locus_tag="ACHKUN_000656"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO6658969.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGIDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(27439..27726)
                     /gene="paaB"
                     /locus_tag="ACHKUN_000657"
     CDS             complement(27439..27726)
                     /gene="paaB"
                     /locus_tag="ACHKUN_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO6658970.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(27738..28667)
                     /gene="paaA"
                     /locus_tag="ACHKUN_000658"
     CDS             complement(27738..28667)
                     /gene="paaA"
                     /locus_tag="ACHKUN_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO6658971.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            28952..30997
                     /gene="paaZ"
                     /locus_tag="ACHKUN_000659"
     CDS             28952..30997
                     /gene="paaZ"
                     /locus_tag="ACHKUN_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO6658972.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGAMRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            31245..33518
                     /gene="tynA"
                     /locus_tag="ACHKUN_000660"
     CDS             31245..33518
                     /gene="tynA"
                     /locus_tag="ACHKUN_000660"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO6658973.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(33576..35075)
                     /gene="feaB"
                     /locus_tag="ACHKUN_000661"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(33576..35075)
                     /gene="feaB"
                     /locus_tag="ACHKUN_000661"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO6658974.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            35311..36216
                     /gene="feaR"
                     /locus_tag="ACHKUN_000662"
     CDS             35311..36216
                     /gene="feaR"
                     /locus_tag="ACHKUN_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO6658975.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(36388..36717)
                     /locus_tag="ACHKUN_000663"
     CDS             complement(36388..36717)
                     /locus_tag="ACHKUN_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO6658976.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(36722..36907)
                     /locus_tag="ACHKUN_000664"
     CDS             complement(36722..36907)
                     /locus_tag="ACHKUN_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO6658977.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(36904..39543)
                     /locus_tag="ACHKUN_000665"
     CDS             complement(36904..39543)
                     /locus_tag="ACHKUN_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO6658978.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNVRLPGNDCPQGKECEEKQ"
     gene            39751..40740
                     /gene="ldhA"
                     /locus_tag="ACHKUN_000666"
     CDS             39751..40740
                     /gene="ldhA"
                     /locus_tag="ACHKUN_000666"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6658979.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            40851..41273
                     /gene="hslJ"
                     /locus_tag="ACHKUN_000667"
     CDS             40851..41273
                     /gene="hslJ"
                     /locus_tag="ACHKUN_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO6658980.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(41270..41536)
                     /locus_tag="ACHKUN_000668"
     CDS             complement(41270..41536)
                     /locus_tag="ACHKUN_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO6658981.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            41810..45334
                     /gene="nifJ"
                     /locus_tag="ACHKUN_000669"
     CDS             41810..45334
                     /gene="nifJ"
                     /locus_tag="ACHKUN_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO6658982.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        11418 a        12106 c        10890 g        11044 t
ORIGIN      
        1 aataaatata ataggcgctt ccgatgtaga cccgtattct tcgcctgtac cacgggtcgg
       61 ttttagtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc atcatgcgta
      121 gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg tgcaacccga
      181 tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta tcaacccgtt
      241 cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac gggttgcccc
      301 ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga taaatcacca
      361 aatgtcccaa atgcgccagt cccgtcatca tcggttttgg cttaggtata atcggcgggg
      421 ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt gagataccac
      481 aggaaacatg aaccatgttg atgagtggcc gatcgctacg tgggaagaaa ccacgaaact
      541 ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg ctgatttgta
      601 accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc gttaacataa
      661 cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg ttacgtttca
      721 ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa ggatcaattt
      781 tcatccgaat gttcctgaca ggagaaaacc atgagtaaag ttggtattaa cggttttggt
      841 cgtatcggtc gactggtgtt gcgtcgatta cttgaagtca aaagcaacat agacgttgtc
      901 gctattaatg atctcacttc cccaaaaatt ctcgcctacc tgctgaaaca tgattcaaac
      961 tacggaccat tcctctggag cgttgatttt acggaagatt cacttatcgt tgatgggaaa
     1021 agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc gaaaggtgca
     1081 gaaatcattg tcgaatgtac tggcttttat acctccgccg agaaatcgca ggcgcatctt
     1141 gatgctggtg cgaagaaggt gttgatttcc gcccctgccg gtgaaatgaa aactatcgtt
     1201 tataacgtca atgacgacac tctggatggc aacgacacca ttgtttccgt ggcgtcatgc
     1261 accactaact gtcttgcgcc gatggccaaa gccttgcatg acagtttcgg gatagaagtc
     1321 ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga tggcccacgt
     1381 ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca cactacgggg
     1441 gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa aggtcatgcg
     1501 caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct cggaaaaaaa
     1561 gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa cgagtcattt
     1621 ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt cggttcggtg
     1681 tttgatgcca cgcaaacgga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc
     1741 gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc
     1801 gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctcttttac tcattacgac
     1861 tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg
     1921 ccaataccgg atttacgcca tccggcgtgg aagccttgca tagcttcgaa gttttcacgg
     1981 ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt
     2041 tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcatct
     2101 tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc
     2161 gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccgtct
     2221 actgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc
     2281 tcgacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca
     2341 gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca
     2401 taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc actattaatg
     2461 acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata
     2521 gctggtgctt taccacccaa ttccaggcac actttggtga tgtttttcgc cgcagtcgcc
     2581 ataatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta
     2641 cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc
     2701 ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca
     2761 ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg
     2821 aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac
     2881 aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc
     2941 cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc
     3001 tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatg
     3061 ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct
     3121 ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt
     3181 atgcgggaaa tgacagcctc tgcagcaggg tttaccacat caatccatgc gtctccacgc
     3241 caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac
     3301 tcctgtgatt tatatgtttg ttttcggtga atgcattgtt aattaacatc aatcatcaga
     3361 gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga
     3421 ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac
     3481 gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt
     3541 cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg
     3601 cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag
     3661 tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc
     3721 tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg
     3781 attgtcccca gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg
     3841 aacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgcga tgctaaagcg
     3901 tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg
     3961 ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat
     4021 agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat
     4081 accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat
     4141 agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc
     4201 ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg
     4261 cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac
     4321 tgcgtgagat aatagtgaga agcggagcac tctgaaagga ggaagtcgta tgtccttaac
     4381 cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac attattcatc
     4441 ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc gttttagaat
     4501 taataaaaaa cccggcgcta aatgcttacc gggttttttc tttatcaggc aaatagcagg
     4561 gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc ggataaggcg
     4621 taccaagttg ttgagcgaag taactaacgc gcaactcttc tatcatccaa cggatctctt
     4681 tcacgtcttc atcctcacga cgtgcgggcg gcagtttgtt gatccattgc tgccacgcct
     4741 gctggacgtt ttcgactttc agcatctgag cacggtcgcg atgtggatca accgccagtt
     4801 tttccagccg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc
     4861 cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta atgtcagaaa
     4921 gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata ttgaacaccg
     4981 cagtgaggat ttgctcgacc tgcttcgcaa tatccaccac cgtgtcattc agttcggcgc
     5041 gcactttttc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca ccattggcgt
     5101 cgatcaattg atccacaccg caggagatac agtcgtcgat cagctccagc actttgccat
     5161 acgggttaaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt aaatatttga
     5221 ttggcgatgg aatattcagc agcagtagac ggcgaagacc gttccacatt gcctgctttt
     5281 gctccagcgg gttatcaaac agtttgatcg ccacactgtc gcgctcatcc accagcgccg
     5341 gccacgcctt cactttgtag ttgccacgct tctgttcgta gctttccggc agctgaccaa
     5401 aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgca gatagcgttt
     5461 cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct tcttttagct
     5521 tcttgttttt gtcatccacc acgcggaagg taattttcag gtgatcaggc acctgatccc
     5581 agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc
     5641 tgtcgagcaa cggtaactcc agcggtttga cgcggcctaa aaacgcttcg gcatagtttg
     5701 gcgcgggtac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga gcaatcacca
     5761 gttcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc tggttaagta
     5821 acggcagcgg aatatgtacg gtcacaccgt cagcatccgc gccgggctca aactgatagc
     5881 tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc ttgctgattt
     5941 tttctgcgcc ctctttgatc aacatgcttt tttcaaagtt gagcaaatca ggggtttcgc
     6001 ggctgacttt tttccaccag ctgtcgaagt gacgagcgga gattacatcg tggctgatgc
     6061 gctggtcgta gaactcaaac aacgtttcgt catcaaccag aatatcgcgg cgacgtgatt
     6121 tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac
     6181 gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacgg cataacgccg
     6241 gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca taaacagtga
     6301 ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgaggttcg ctgtaggtgc
     6361 gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc gcagcaatgc
     6421 gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc ggcggttttt
     6481 tgaataaacc agaaccggga aagatggaga aacgcgcgtt acgtgcgccg gtatattctt
     6541 gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac gcaatgtgaa
     6601 tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc accacctggc
     6661 gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga taatcgatac
     6721 gacacaggcg acggaaggcg ttggaagaaa gcgccttttg ctgctcgcca agataattcc
     6781 acagattcac aaacgcgagg aagtcagact ctttgtcgtg gaagcgacga tgtttttcgt
     6841 ccgatgcctg ctgtttatcc atcggacgct cgcgcggatc ctgaatggaa agcgcggacg
     6901 tgataatcat cgcctcacgc acgcagccat gtttttgcgc ttccagcacc atacgcgcca
     6961 gacgtggatc gacaggcaac tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc
     7021 tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg ccatcctgga
     7081 tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc agccccagcg
     7141 cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga tcggtaaact
     7201 ccgggcgcga aaggaaatcg tcttcggaat agagacgaat acagatccct tcggacacac
     7261 gcccacagcg gcctttacgc tgattggcag acgcctgcga aatgggttca atcggcaaac
     7321 gctgcacttt ggtgcgatag ctgtagcggc tgatacgtgc tgtaccgggg tcgataacgt
     7381 atttaatccc cggtacggtc agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc
     7441 gtccgctgtg cgactggaac acgcggttct gttcgctgtt cgaaagccgc gcataaagcg
     7501 gcaagatttc ggtatgacgc agatttagct tgttcagcgc atcggcggta tcgcgaattt
     7561 cccgctcacc actcataaag atcagaatgt cgccagggct ttcctgactc agttcgtcta
     7621 cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc gtcggcttct tcaacaatcg
     7681 gtcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc ggcgcattat
     7741 taaagtggcg cgaaaagcgt tccgggtcga tagtcgcgga agtgataatg atttttaggt
     7801 caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata ttcaggctgc
     7861 gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg tcttgctgga
     7921 tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca ctaacgtgat
     7981 cgctgaaacg cactttgtaa ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa
     8041 tacggttcgc caccgttctt gccgccaggc gacgcggctg ggtatggccg atcagccctt
     8101 taatcccgcg ccccagctcc atacagattt tcggtaactg agtcgtttta ccagaacccg
     8161 tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaatg tcctgttttt
     8221 tctgactaac cggtaaattg tcaggataag taatttccgg tcgtgccgct tcacgcagca
     8281 ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg
     8341 catcaggatt tttaaccttc ttaacaccgt gcagacgccg agaaaaacgc agtctgtcgc
     8401 gcagcatcag cgaatccagc cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg
     8461 tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga tagatataga
     8521 gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc ttcgatttat
     8581 tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg gcatcgccca
     8641 ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc cagcatcctg
     8701 gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag
     8761 cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc ggtactggat
     8821 ggcgaactgg ttggtgcact gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa
     8881 gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt tatcgttatt
     8941 gcggtaccga tgtataactt caacatctca actcagttga aaaactattt tgacctggtt
     9001 gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct ggtaacgggt
     9061 aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc aacggacctg
     9121 gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt gaaatttgtc
     9181 ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa
     9241 gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg gactgtagtt
     9301 cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc tttgtgatct
     9361 tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc atcaacggtt
     9421 ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg cgataatccc
     9481 aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag catcgccacg
     9541 gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac acagaaatag
     9601 agtcgatctt ttgtcgctcg tccccgaggg aattcaaagg tgacgtccag aaccgcattc
     9661 tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtaat tttgctcacc
     9721 ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg ccagggcaat
     9781 gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt tgttatcgcg
     9841 ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca acataaccag
     9901 actgaccacc ataactgaat catcaccatt agctccatca acacaatgca cgagcccgcg
     9961 cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa
    10021 cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca
    10081 tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa aaaccatccg
    10141 ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca aagtagccag
    10201 cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc
    10261 gaaaatagcc atcccgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga
    10321 tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg
    10381 cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag atccagactc
    10441 cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga
    10501 ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag agatccatag
    10561 gtgaagaaga aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc
    10621 cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc actcatcaat
    10681 ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct ccccttgcga
    10741 acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcat
    10801 ctccagttga gggtgccacg gctgcccggt gtagatgaga atgccgcccg gttcgatggc
    10861 attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatacag
    10921 gccagagaca atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa
    10981 cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatcgcttg
    11041 cccctgtgca acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc
    11101 gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggac
    11161 ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt gtaaatgggt
    11221 tttacgctgg cgaataccgc gccagccaac actgttcagg tagattttgt caattaagcg
    11281 cccgaatgcg ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga
    11341 atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgccccga acaatttcat
    11401 cgcctttcgc ataaagcgat acgccaaatc aaccggcgat aatggcacgg gtccacctga
    11461 aaggagccgc cagcgatccg ctgatggtcc agtgcggtct tcatgctgat aatcaaattt
    11521 ttgtgacttg ttagcatata aacgactaat aaagctttgc attttttcaa atgcctgcgc
    11581 cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttcag
    11641 agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc
    11701 gcctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatctctaa taatacgttc
    11761 agacgttttg tagagatcga gcaagatatt aacggcaatc gcccgtgtga tcagcggatc
    11821 attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga
    11881 attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg
    11941 tacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa ttgcaggcgc
    12001 ataatcatga acccatgtgg caaccagcac tgcgccgacg ctttgcgcga tcactaccac
    12061 ctcatccagt ccgacctggc tatcgctggc agcaaaacga acaaattcat cgacatcctg
    12121 cactgaacgc gcaagagatg ggctataacc acgcggcccc gaagtttgtc catgccctcg
    12181 ggcatcccag gcataaaaaa cagtatctgg catcgccagt tcatcaacga gatgttgtag
    12241 acgaccagaa tgttcatgac cgcgatgaaa taagacgatg acctttttag ccccgggctg
    12301 taaagcgggc cagtgccgat aaaacaacgc tgtattatca ctgctggtaa aaaaatgttc
    12361 cccatggatg cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat
    12421 aaaataaaaa aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc
    12481 atgtcctggt aatagtttcc cactatcttt aacaccaata tctcgtttga tggctgacat
    12541 cacgacatcg ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa
    12601 tgtatttagc ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac
    12661 gccccccacc agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct
    12721 gccgcaggat ttcccccata aatactgtgc aatatcgttt gactcggtta atgccaaaag
    12781 aaataacacc agtaacgccc cgccctggat atttgtggtt ggcaacatta ataaccaggc
    12841 ggcatgactc aaggcgaaaa cggtcattat ccagccccaa aaaatggcgc tcacggtatg
    12901 cagaaaaccg gaggggtccc ccataaaaac ttgcccggtg gctaatatta gaaacccagc
    12961 caaaggtata aataatagat acagctcaaa gcaattaaaa ccgataaaca aatagttaag
    13021 aggaataacc caatataacc accgcggaaa atgtacagat ataagcgtac aatattcttt
    13081 caatgccaga aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg gacctgaaat
    13141 caccagtgaa aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc
    13201 ctcttttctc tccggtaacc gccatagcag aaaatggttt atcagcgttg ctaaaattaa
    13261 caacgcgaaa agtgttgcca gagatttttc cagcattatc tttcagcgct cataagaagc
    13321 acactccgac aacgattaat cgcagtccag agaagcagaa ttgaagcaat gctccataaa
    13381 agattattcc actgcatcca ctgtggataa atggcaacgg ccagacccca cagaccaaat
    13441 attaacgcgc gatcgctttt gccaaatgga ccggcataac tgcgaacacc gttaatcgtc
    13501 tgcgcgagta aaccgcaaaa ctcggtcaat atggtgcaaa ataacatgag tatcacgagt
    13561 gatgcgttac tttccggtaa aaataaaaat ggtaaataga gcgcaatatc ggaaataaca
    13621 tcaccagtct cattcaaaat tgccccaagc cgtgtttgct gattgcactc acgcgccaac
    13681 atgccatcca gcgcattgag cgccatacgg ataaaaagca cgatgggcaa tagcataaag
    13741 aggatgggtt gtgccaccaa catcaacagc aatccggtaa gaagagaaag cgccagtgca
    13801 gcaagagtga tgtgattcgc tgtaacgtgg tgcttgtaaa gccaaaacat cgtcggcctt
    13861 aacagcgact gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc
    13921 ggtttgcgct atggtgcatc aattcccctg acggagatat cgcaacacgc cgcttcatta
    13981 aaaattcaga atatatcaag gtattgggga ctcactgtca ggaataatta tccccaatta
    14041 atcaaagggt taagaaatat tacgttgtgg atcaatacaa ctcatcaagt tttctcatcg
    14101 cttgttcccg tattgcgcgc gtcttaccgt taagcgccag ctggcggact aaatccagtt
    14161 cagggttaac aagctctaac gccagcaaag gatcacgttt cagcctcgcc tttaatacat
    14221 caactggaaa acggggatta ttcatcgcca caatccacca ctcgggatca atttctgccg
    14281 ccagtactgc aggcggtgtg tggcggttgt taagtgcgcc gaacttgaaa aatgtgagat
    14341 tgcttttcgc cagtgtactc caccatatac gatccggttg ttttgccagt acatccagtt
    14401 gtgctaacgc atcggggtcg agtgcataat agacgcgtaa tgcttcatca acctgcgcac
    14461 gggagctgtt ttcctgcctc agaaaaaact tcgcccaaat atcattgcgt ggcgtatttt
    14521 gctgtaattc cttgaggaca acagcgggta aattatccca actaactaat gcactctcta
    14581 aaaagtccca gtcttccaaa cgtatcaggg cggtggcatt taatagcatc tgatcatcaa
    14641 cttcaccgtg gctgataagc tcagacaaaa tcatatcagc gctggtttca tacgcagctg
    14701 aatctgcttt cggattattt ttccgatgcg ataaagcacg ttgccaaatt ttttgtttca
    14761 tcgacgtgct cagatactga tttttcgcca gattactcca cccctcagga tcgttgaaac
    14821 gtgtcaataa ccgttgtaga gcagcgtcag agtgaattac ctgcatatcc cgacagcaaa
    14881 agtaggcagg ggattttttc tccatccttt taacaaaatt aaactgtaag ttatctggca
    14941 atgctaaaaa taacacacgt attagatcat cgtcattctc gaatttttgg tataaatagg
    15001 ctgcaacttt gtcactctct tgtggcgtca tccgcaataa ttgaccatgg tgtttcatct
    15061 ctgccagctt gaacgctaag cttgtgacaa cattgtttga tgaatcgttc gccaactgca
    15121 ttgcctgatt gtaggtgagt tgtcctccag caccgacctg acgaatagag gaatcatcac
    15181 tgcggatata gccatcaata atggattcag gcaataacca cgcatcttta taaagctcct
    15241 gaatgatcat atcccaatct cttttcgcca gagtcattac gatatccaga gggagatttt
    15301 cacgtagcgc aagattcaac ggaccgacac catcaggtag acgactcatt cgcgtcatta
    15361 tttcgacaga gagcggttca cggtcgagca accccagaac aacaggctgg taacccaatt
    15421 gctgatcggt caactccaga atctcattgt caggcatata tctcgccata ctttcaatcg
    15481 tttgttcatc attgctatta ataagttgtt tgcgtaggtt agctgaaatt cctttaagcc
    15541 ttgggagagt tcgcattatg gcaagatcaa atgtcgcaat atctcgtcct tcaaatgcag
    15601 caacgttgcc ttcatccagc atttgcaatt cctgcactaa ctcaggagaa ggcttagggc
    15661 gaaacagcgc ttcttttttc atctgattac agacattccc tgccatgcga atattgcttc
    15721 gcttaaaaat ttctttcaga gcatcttcat catttattag accccaataa tatgcatcag
    15781 gatttattat catcagcgct tttttgacat tttcagtgtt atttttgtca ctcgcacgga
    15841 ccatgaattc actatttgtc gaagacgcaa caacaggtgc aattatgtca ttgataaata
    15901 ttccacgcgc attaatactc cccttaatcg aagacggatt atcgtaatat gagtcgataa
    15961 ataccagtcc acttgcattt atcgaaccgg taattaacag tggtgaatcc gtaactatat
    16021 tcgttgccgt cacgttaccg agaataaccc atggattaga aagagagaaa gtggaaatat
    16081 tacctttgac tgtcaaatta ccaagaacaa tcaatttttg attactctct atattacctt
    16141 ctaccactaa atcgccacga tgcactttcc agtccgatgc agataaaccg aaggggacaa
    16201 gattcacgct actgttgata gcttgttcaa cttgttgttt atcttcagcg gtagccgcag
    16261 aaaaaaatgg ttcaatatca actgtatttg caacacatcg aagttccgcc tgtactggaa
    16321 gactcagcat aaacagggga agtaaaccta tccagccgca aatcctggac ttaaacatat
    16381 gcactccatg ccattaaaaa agaaacaaaa gggaaatgca tctttccctt ttgtatattc
    16441 gttattctcg cgaaatacca tccaacgtag acaacacttc ctcagaaaga tgcaattttt
    16501 cagcagccat attctcccgt aaatgcgcaa cagaagacgt ccctgggatc agcaaaatat
    16561 tcggtgaacg ttgtaacagc cacgccagcg ccacctgcat gggtgttgca cccaggctcg
    16621 cagcaacatc cgacagcgta gaggattgca gcggtgtaaa gcccccgagc gggaagaacg
    16681 gcacgtaggc aatgccatcg tgggccaaag catcaatcat tgcatcatca gcacggtgcg
    16741 cgatgttgta ttcgttttgc acacagacaa tttcggcaat cttgcgcgcc tctgcaacct
    16801 gcgtcggtgt gacgttgctc aggccaatat gttttaccag gccttgttgt tgcatctctg
    16861 ccagcacggt caggctggcc tcaatcgatc cttccgctgg gccatgacca tctcccatca
    16921 taacgcgcag gttaaccaca tccagtacgt ccaggccgag attacgtaga ttatcgtgca
    16981 ccgctttttg caactctgcc ggagaaaagg cgggcaacca ggaagcgtcc tctccacgcc
    17041 gcgcaccaat tttagtgaca attgtcaggt cgtcagagta aggataaagc gcttcgcgga
    17101 taatctgatt ggtgacgtgc ggaccataaa agtcgctggt atcaatgtga ttgacgccca
    17161 atgccagcgc ctcgcgcagc acagttatag cgacgtggcg atctcttggg ggaccaaaaa
    17221 ccccaggacc tgccagttgc atcgcgccat aaccaagacg gttaacggat tttgtaccga
    17281 gagtaaatgt attgctgctc atcgcaccct gccctgtaat aaaacggatt aagaaaaatc
    17341 agtatagaaa cgaaaagaga taaataatgc gagggcgtaa acaagattac tggatgagtt
    17401 gctctattgt cctgaaaaac acaaaacaaa acgcgtaaag cgccgcatcc ggcaataaaa
    17461 aaaccgcaac ctgtatcagc gttgcggttt aggtatcaca tgctaaaaat tagaatgttc
    17521 agtatctgga cgttactgtt tcggtcgcag attctcatca aataccagac gtttcctgtt
    17581 cggttcaact tcccgcaatg gctcaacttg atgcaacgtc tgcttacaac gagtcaccag
    17641 cacctgatac tcatgcgtac cctgcttttt ccatgccagc tcctgctcac tgagttcacg
    17701 aatcgctttc gccgggctgc cgacaatcag gtaattggca ggcatttctg ctttggcttt
    17761 cacaaatgcg gatgcgccaa caatgctgtt ctcgccaatc accgcgccgt ccatcactac
    17821 cgcgttcatt cccactaatg cattgcggcg gataatgcaa ccatgaagga tagcgctatg
    17881 accaatatgt ccatcttctt ccacgacagt atcctgctcg ggaaaaccgt gcataacgca
    17941 attatcctga atgttcgcgc catctttcac cacgatacga ccaaaatcgc cacgcaggct
    18001 ggcatttggc ccaacgtaaa cgcccttgcc tagaataaca tcgccgatca ataccgctgt
    18061 cggatggaca aaactctctt ctggcacaac cggagtcaga ccatctatct ggtaaattgg
    18121 catatcgcct cctgttcaat attcaaaccg ccaaaacgtt gaaaatacag gcttcccggc
    18181 gcaggcagtt caccgaccga ggtttcacct ttttcactaa cgaacgctaa agcagcaggc
    18241 gctacgcgct gataaatgtt gatacacagc tgacgcgccg tatgccctgc ccagtgcgcc
    18301 ggaagcaact cctctggcaa caatgggtct ttaaggacga cacggcgata aaaatggatc
    18361 agtaaaagct gaatatgaaa tgcacgctcc ggggttaact cgtctgctgc ctcttttaac
    18421 agcggcacca gcgggcggaa tgactgaata aaggtttcgt acatggcatt ttgttcagtt
    18481 aaatgccagc actcttccac tctggcacgc agtgctgcac gagaaagcgc cagtggtatt
    18541 tgcgcttcaa aacaaatcac gttatccgcc acccccgctt catgcaaaag tgtctgtaca
    18601 tccgccagtt tttgcgacgg cgatgccatc aggctgggtg ccagtgcgcc aaaaccttgc
    18661 cagatcaact gctttttgac atcagccagc gttgatttat ctaaaccttc cgagagcaac
    18721 aggagccatt taccatccca tgcaggttgc tctgcgcgat aaattttact ttctgcccgt
    18781 cgcgtcaggc gcaagccttt atcactgagg ctatagaaac tgcgtcgccc gatgcgggaa
    18841 acatccagcc agccttcttt attaagacga aacaaagcgg tgcgcacgaa acgctcacca
    18901 aatcccagcc cttccagcaa agcagccaga ctacccagcc agatttcacc accacgatgg
    18961 gaaagcgaat caccatacag agaggagatc aaagatgtgc cactgaccgg aacagcgttt
    19021 acagcatgtt ggataaaagt aacaagttta ctcattcgat tcagtctgtt gttattgttg
    19081 tgatttatga atcatagcac aggtcgtatc gacttctggc cctggcgctt tacctcacca
    19141 gggccagacg acacgtcagg caccaacaat attgcgcaga tcaaacaccc gacacgcctt
    19201 gccttctgaa cgcgggatac tgccacagtt aacgatcatc acatcggtag agatccccac
    19261 catcgactta atccgatggc gcagctggtg gcacacctgg caacgctgct catgcgtcaa
    19321 tgtcaggctg ctttctttca actccacttt cacagaaagt gaatcaagat gcccccggcg
    19381 gttcacctcc agttggtaat ggggcgataa atgttcgaat ttgacaatct cttcttccag
    19441 ttgtgacggg aagacattta caccgcgaat gatcaacatg tcatcgctgc gtccgctgat
    19501 gcgatccatg cggcgcatag tccgcgccgt tcctggtaac agtcgggtta aatcacgcgt
    19561 gcggtaacga atgaccggca atgcttcttt ggtcagcgtg gtgaataaca gttcgccatg
    19621 ttcaccatcg gcaagcggtg tgccgtcatg aggattaaca atttcaggat agaaatgatc
    19681 ttcccaaatg gttgggccgt cggtagtttc cagacactcc atcgcccccc ccggccccat
    19741 cacttctgac aggccataaa tatccagtgc ggtgatcccc agacgccgtt caatctcttt
    19801 acgcatggcc tgtgtccacg gctcagcacc aaacactcca acccgcagcg agcaaccgct
    19861 ggcatcaccg cccaactgcc gctccagctc ttcaatcagg ttaaggcaat aagatggcgt
    19921 aaccatgatc atatctggtt gaaaatcgcg gatcagttgc gcttgttttt ccgtctgccc
    19981 accagacatc gggatcaccg tagcgcccag acgttcggca ccatagtgcg cgcccagccc
    20041 accagtaaac aggccgtaac cataggcaac atgaattttg tctttgggcg tgccacctgc
    20101 cgcacgcaag gaacgcgcta caatattggc ccagttatca atatcgtttt gcgtatagcc
    20161 gacaactgtc ggtttacctg tggttcccga agaagcatga atgcgcacca cttgttccat
    20221 cggcacggca aaggtgtcga agggatagtt gtcgcgcaga tcctgtttgg tggtacaggg
    20281 aaatttacgc agatctgaaa gttccctgaa atcatcagga tgtacgcctg ctgcgtcaaa
    20341 tttgcgccga tacatcggga cattttcata cgcgtgtttg agcgtccatt tcagacgttg
    20401 tgtttgcaac gcctgtaact catcaacaga cgcggtttca atcgggtcaa gctttgtatt
    20461 ggttatcatt gtagggtact cgcaggttga tatgctcaaa cacgctccag aatcatggcg
    20521 atgccctgac cgacaccgat gcacatggtg cacaatgcgt aacgaccgtt acgccgatgc
    20581 agctcatggc tggcagccag tgccaggcgg gcaccactca ttcccaacgg atggcctaag
    20641 gcgatagcgc ctccgttggg gttaacatgt ggggcatcat caggcagccc caattcgcgt
    20701 agtacaccca acgcctgggc cgcgaacgct tcgttcagtt caatcacgtc catatcgtga
    20761 atactcagcc ccgcgcgttc cagcacccgg cgagttgcag gcaccggacc aagccccatc
    20821 aggcgcggtt ccaccccggc ggttgccatg gctacgattc gcgcccgcgg tgtcagtcct
    20881 tgcgctgctg ccatctgttc actggcaata atcaacgcag cggctccgtc attcaccccg
    20941 gaggcattgc ctgcggtaat caccccattg gcacgaaatg gtgcttttaa cccacgtaac
    21001 tgttccagcg tcgtttccgg gcgcagatgc tcatcatgtt gtatttctgt tacaacgcct
    21061 ttcttgtttt tcaacacaac cggaacaatc tcctcagcca gaatgcctga ggattgcgct
    21121 tttgccgtac gttgctgact gcgtagcgca aaactatctt gatcttctcg cgatattttt
    21181 aacagttcag ctacattctc tgccgtttcc ggcatgctgt cagttccaaa ttgttgagcc
    21241 atgagcgggt tcacaaatcg ccagccaata gtggtatcaa acatctcagc ctgacgagaa
    21301 aatgcactgg ttgccttgcc cataacaaac ggtgcccgag acattgactc cacgccacca
    21361 gcgatcagca aatcgccatc gcccgcttta atcgcccgtg cggcgaaccc cagtgcgtcc
    21421 agcccggaac cacacaagcg gttaatggtt gtgccggaaa cactatgcgg cagccccgcc
    21481 agtaacgtcg ccatccgggc tacgttacgg ttgtcttctc ccgcctgatt agcacagccg
    21541 aggatcacat catcgataca ctccgcatcg agacgcgggt ttcgcaccag cagctcccgc
    21601 aaagggatag cagccagatc gtcagcccga acacctgata atgccccacc atagcggcca
    21661 attggcgtac gaattccgtc acagataaag gcttcacgca tcaggcttct cctgtaatgg
    21721 tgccgccgat gcggtgagat ttaccgcgaa acagcgcaac cgttttttgt tgttggttaa
    21781 caatttcgat gtcgtaaaca ccggtttgct tgccctgatg acgtacctgc gcagtagcag
    21841 ttaaggtgtc tccggcaaag cctggacgca aaaaatcaat cgtgcaggca gaagcgacgg
    21901 ctgccagccc ctggctattg caggcgtagg caaaggcagt atcagccagt gaaaatagct
    21961 gcccgccgtg gcaactttga tgaccgttaa gcatttgtgc agtgacggtc atggtcacta
    22021 cagcaaagcc ttcatccatt gagataatgt cgatgccaag cgctttggcg caggcatcgt
    22081 tctcatacat tgcatgggca ttttgccagg ccttatgact cataaccgct ctccagaagc
    22141 gctcgttggc gcagcaatga acatgggcga tagcgttctt cgccatagtg atgttgtaga
    22201 ttttccagga gccttaatat tcgctgccag ccaagttgcg ctccccaggc aagcgggcca
    22261 tatggataat tcaccccaag acgcatggcg gtatcgatat cctgttcaga ggccacgcct
    22321 ttttgcagcg catcaagggc ttcattgatg atcattgcta ccgttcgcca aatcagcatt
    22381 cctgggtaat ctgcaatttg cagcactgtt ttgccctgct gttgcaggta ataaatggcc
    22441 ttgcgggtcg ctgagtccgg gttcaccgct gccgcagcaa tggtcaccac cttgcccgcc
    22501 attttgtcga ccactaccac cgggcgtgcc agtcgtattg ccagcgcctg tgccgtctcg
    22561 ccttgtgtct caatcaataa aacatcgtca atttccgtga cgccgtcact tttcttttct
    22621 actttcattg ggctaaaact gtcgcttacc gcttccaggc caacaactgc ctcacgttcc
    22681 gcgcgccagt cgtacacgcc cagcccactt ttcttgccca accgtccacc aatcaccagt
    22741 tcctgttgca ccagcgaagg taaaaaacga cgctcctgcc agaaagcgtt aaacaccgaa
    22801 caggtgacag caaaattgac gtcctgacca atcagatcgg ttaattccag cggccccatc
    22861 gggaaaccag cgccatcgcg aagtgcagcg tcaatcactt ctggtgcagc aacctgctct
    22921 tccagtgccc gccaggcctc ggaataataa ggacgcgcca cacggttaac gataaatcca
    22981 ggggtcgaat gacagcgcac aggctgctta ccccaactca acgttagttc acacaactgc
    23041 tcaacaactt ccgctgccgt tgccagccca ctgaccacct ccaccaactt catcaccggt
    23101 gccgggttaa aaaaatgcag ccccgcaaca cgttcaggat tttttacctc cgcagcaatc
    23161 gcggttatag agattgacga agtgttagtg gtcaatagcg tttgtggcgg gcaaacttcc
    23221 gccagctgtg caaagagcgc ttttttgact tccagacgtt cagacgccgc ttcaatgacc
    23281 aggtccgcag ctgccagcgc gtgaatatcg gtcaccggga tcaggcgttt caatgtgcgt
    23341 tcacaggttt cagcagtcag ttttccccgc gtcacgcgtg aatttagccg cgcgtgtatc
    23401 ccgtcgattg cgcgggtcag cgcttcagca gaaatgtcat acagtaaaac ctgatgtcca
    23461 tgactggcgg caacttcagc aatgccagcc cccatggtgc cgctcccaat cactgccaca
    23521 gtttgcacat ttatcatcat gctatttccc cgtgaactgc ggtgagcgtt tagccaggaa
    23581 cgcactgaca ccttcacgat aatctgcgct ccgtccggca agtcgctgat agtcgcgttc
    23641 cagatccagt tgcgtatcga gcgtattggt ttcagcgcta tttatcgctt gcttgataag
    23701 tccaagacca aatgtcggtt gtgtcgccag atgccgtgcc agctgttgcg cggtatctgc
    23761 cagcgtttca tcatcaacaa cctgccagat catcccccat tcgtgcgcct gttcagcact
    23821 cagttgattc cccagcagtg ccagccccat ggcgcgcgct cgcccagcaa cgcgtggcag
    23881 taaccaggtt ccaccgcaat cgggtattaa gcctaactta ctaaacgcca tgacgaattt
    23941 tgctgaacgg gcagcaataa cgatgtcgca ccccagtgcc agtgttgcgc ctgcccctgc
    24001 cgccacgcca ttgactgcac agatcaccgg ttttggcagt tttgccaggc gacgtaccag
    24061 tgggttatag aaacgttcaa ctgacatccc taaatccggt gcggggcctg tgggatcaac
    24121 gttacgatcg ttaagatcct gaccagcaca aaatccgcgc ccggcacctg taagtaacag
    24181 gcaacggata gtgtcgtcgc gctcgacctg tttcaggcac tctgccagtt gtgcgtgcat
    24241 ctcatcatta aaactgttca ggcgttccgg gcggttgagc gttagtgtca tcacgccctt
    24301 ttctacatga ctgaggatga attccatcat tagcgtcctt taaagtcggg cgtgcgtttt
    24361 tgtaagaaag cggagatgcc ttcatgacga tcttctgttg ccgccagcaa ggtgaataac
    24421 tgtcgctcct gggcaagtcc ggcttgcaaa gccacttcct gcgactggcg cagcgcttgc
    24481 tttgccgctt gtaaggccaa cggcgagtga cgtgccattt tcgatgccag ctgtaaggcg
    24541 tattcgaggg ttaaatcgct ggggaagacg tcgctaacca gcccggcctg ctgtgcttgc
    24601 tgggcggtga tactttctcc gctcagcacc attttgctgg ctaacgattt accgacacta
    24661 cggattaaac gttgcgttcc gcctgcgcca ggcatgatgc cgagagtgat ttccggcaac
    24721 ccaaaacgcg cgttctctcc ggcaaccacc acatcgcaca acaatgccag ttcgcaaccc
    24781 gccccaagcg cgtaaccatt gacggctgcg ataagtggtt tattaaaggc ctgcaatcgc
    24841 gcccatagct gcggacgtgt atcgtttaag gtggccgcga gatctttttc tgccatttcg
    24901 ttgagatcgg ccccagcggc aaaaaagcgt gcattaccgg taatcacaca gaccgaaatg
    24961 ctggtatcgg tagccgcagc ttccagttca tttaccagtt gcatcagcag ggcattattt
    25021 agcgcattac gtgcggcggg acggttaagg gtcagcaaca atactcgttg ctgacggctg
    25081 acgatcagtt cgctcatgcc atccccttcg cgtcaaagtc aaccaccaca tcgctggtca
    25141 gcggcagtgc ctggcaactc aacacataac ctgcggccag ttcatccggt tccagactgt
    25201 aattggtttc catcgccact ttgccacgca gcactttgca tttgcaggtc gcacagacgc
    25261 cgcctttgca ggcatagggc agatcagccc cctggcgcaa tgccgcatcg agaatgcttt
    25321 catcgtcggc attaagcacg atttcccgat cccgcccatc ctgacgtaca gtcacttttt
    25381 gtccgtcact ttgcacgtta acgctacgtt tgacgcgcgt gccaggcgta ttaaaccgct
    25441 ccagatgaat ggttttatct ggcattccca gtgcttttaa ggcggcttcc gtctcatcca
    25501 tcatcgccgc cggaccacaa ataaatgcct catcataaag acgaaaatta atgagcgagg
    25561 ccccaagtga ctgtaatctt tcaccgtcaa tacgcccgtg aagcagatcg ctgtcgaggg
    25621 tttcctgact gaaaatgcac aacaactgta aacgctgagg atatttgtct ttcaggtctg
    25681 ccagtgcctg gcgaaacatc atgctctggc tggtacggtt accgtagatc agggtgaact
    25741 gactttcagg ctcggtttgt aaagtggtgg cgataatcgc cagcattggc gtaatacctg
    25801 atcctgctgc aatagccaga tagcggccct ggcgttcggc ctggggctga tagccgaaat
    25861 gcccctgcgg caccatgacc tccagcgtca taccctggcg gatgtgttcg cgggcatagc
    25921 gggagaaacg tccgccttca atggctttca ccgccacact aatttcgcca ggcaggtagc
    25981 tgcggcaaat ggagtaacaa cggcgtaatt cttcaccatc aagactggct tttaaggtca
    26041 aatgttgacc ggggcgaaag cgatacgcct cctgcaaagg ctggggcacc gcaaaggtaa
    26101 tggtcaccgc atcacgggtt tccggctcga cttttgccac tgttaaggaa tgaaacgttg
    26161 tcatggcatc ctcaaataca tttgaaataa tcgaaaggtt cgcggcaact atcgcagcgg
    26221 tacaatgctt tgcaggccgt ggaaccaaat tcactgataa gtgtggtatg gacgctggcg
    26281 cagcgcgggc aacgtacttc tggcggcaaa tgggcatggc aactgtgtcc ggcgggcggg
    26341 ctaatgccat actgtcgcag acgctcacgg gcatcggggg tcatccagtc ggtggtccat
    26401 gccgggtcga gttgcagcac aacctgaacg ggggtaaacc cgtgggttgt cattgcctca
    26461 cgtatcgcgc caatcaaatg ttccgttgcc ggacaaccgg aataagtcgg cgtaaagccg
    26521 atcacccatc cttctcccat ctgtgtcaca ttacgcacca tgcctaaatc ggtaatggtc
    26581 agcaccggga tctccgggtc cgggatctgg ctgagtagcg cccatatctc atgaacctgt
    26641 ggcggtgcaa tggtggccag acgttgcata cccatctcct ctgttaccat tgctgaccgg
    26701 gcaagacacg ctggagatac tgcatttctg ccagcatggg tccaaggtgt tcggtatgca
    26761 gtcctttttt accgccagtg cgatacgcct gctcttgcgg tacgttcaat gtggcttcgt
    26821 tgattccggc aaaaacttcg gcttcccacg ctgcgcgtaa agtgcgtgga tcaaccgcaa
    26881 taccctcttc actcagtgca atatcaatct cgtcggcatc gaacagttcg gcagtaaaac
    26941 gccacaactt gtcgatggcc tgctgcatct tttgccctga tacgtcaata ccattgccca
    27001 gtcgctccag ccagccacga ctaaaacgca ggtgataacg cgcttcttta attgctttgg
    27061 cagaaatcgc cgccagttgc ggatcacggc tttccatcag acgggtaaag agcgccacat
    27121 gccatgcatc gatgaaatac tggcgtgcaa tggtgtcggc aaaattgccg tttggttgtt
    27181 caaccaacaa taagttgctg aactggcgct cgtctcgggt gaaggccagg gtatcttcat
    27241 caccttctcc cgctaattca gcggcatacg ataagaagtt acgtgcctga cctaataaat
    27301 cgaggccaat gtttgccagt gcgagatcga tttccagttc cggtgcgtga ccgcaccatt
    27361 cccccaaccg ctgggagagc accagatagt tatcgcccag gcgcaaggtg taagccgtta
    27421 actgattcat ttccgacctc acatgtgctc aatgccatca gggatggtgt aaaacgttgg
    27481 atggcgatag accttgcttt cagccggatc aaaaaattca ccgcgttctt ccggttgcga
    27541 ggcaacaatt tcactcgcct tcaccaccca aattgaacat ccttcgctac gacgggtgta
    27601 agcatcacgg gcattttcca gtgccatccg ctcatcggca gcatgtaaac tgccaacatg
    27661 gcggtgtgat aagccctgtt tgccacgcac gaaaacttcg tataacggcc agtaaacatt
    27721 actcattttg aatctcctta tgcgacctta cgggcaagtt gtttttgtgc atgggccagc
    27781 gctgcttccc gtacccaggt accttcttcc caggctttac gtttggcgtc gagcctctcc
    27841 tgattacaaa ttccgcgacc gttaattact tcgttaaact cctgccagtc gatctcacca
    27901 aagcggtagt gaccgctttc agtgtcaaaa tgcagatccg ggtcaggaac ggtcataccg
    27961 agcatttcaa cctgtggaac ggtgttgtcc acgaaacgct ggcggagttc gtcgttggtg
    28021 aaacgtttga ttttccaggc gagacttctg gcgctgtttg gcgagttatc atcgtttggc
    28081 ccgaacatca ttaaggctgg ccaccagaaa cggttaatgg cgtcttgcaa catctgcttt
    28141 tgagcttcgc tgccctgcgc cagtgccatg caggcttcaa acccctgacg ctggtgaaaa
    28201 ctctcttctt tgcagatttt caccattgct ctggcatacg ggccataaga agttcggcat
    28261 aacgccacct gattgacgat cgctgcgcca tccaccagcc agccgataac gccgatatca
    28321 gcccaactca atgtcggata attaaagatg gaggaatatt tcatccgtcc gtcgagcatt
    28381 ttttggtaga tgtcttctct ggcacagccc agtgtttcgg cggcgctata gagatacaga
    28441 ccatgaccgg cttcatcctg cactttggct aacagaatgg ctttacgccg caaggttggt
    28501 gcgcgagtga tccagttacc ttcaggcaac ataccaacaa tctcggagtg cgcatgctgc
    28561 ccaatctggc ggatcaatgt ctttcggtaa gcatcgggca tccagtcctg cggctcgata
    28621 gccgtttcct gggctatccg ttgctcaaag cgttcttctt gggtcacgtc atcaccttta
    28681 cgattccagt tttcgtgaat taattaaaaa cacgaatcac gttgttttat gaaagttaca
    28741 caatggttaa ctaaagtcac aaattttgta tacttgaact gtgacagatt tcgcactcta
    28801 ttttgataac tcattattta tcagtgcatt aatgttttta gtagttgata tacagatcac
    28861 gcagttaaca tttcgttaaa agatcccttg ctttttatga ttcgcgattt aactattagc
    28921 aacagaaatg tgaaacatct ggagagtagc gatgcagcag ttagccagtt tcttatccgg
    28981 tacctggcag tctggccggg gccgtagccg tttgattcac cacgctatta gcggcgaggc
    29041 gttatgggaa gtgaccagtg aaggtcttga tatggcggct gcccgccagt ttgccattga
    29101 aaaaggtgcc cccgccctcc gcgcgatgac ctttatcgaa cgtgcggcga tgcttaaagc
    29161 ggtcgctaaa catctgctga gtgaaaaaga gcgtttctat gctctttctg cgcaaacagg
    29221 cgcaatgcgg gcagacagtt gggttgatat tgaaggtggc attgggacgt tatttactta
    29281 cgccagcctc ggtagccggg agctgcctga cgatacgctg tggccggaag atgaattgat
    29341 ccccttatcg aaagaaggtg gatttgccgc gcgccatgta ctgacctcga agtcaggcgt
    29401 ggcagtgcat attaacgcct ttaacttccc ctgctgggga atgctggaaa agctggcacc
    29461 aacgtggctg ggcggaatgc cagccatcat caaaccagct accgcgacgg cccaactgac
    29521 tcaggcgatg gtgaaatcaa ttgtcgatag tggtcttgtt cccgaaggcg caattagtct
    29581 gatctgcggt agtgctggcg acttgttgga tcatctggac agccaggatg tggtgacttt
    29641 cacggggtca gcggcgaccg gacagatgct gcgagttcag ccaaatatcg tcgccaaatc
    29701 tatccccttc actatggaag ctgattccct gaactgctgc gtactgggcg aagatgtcac
    29761 cccggatcaa ccggagtttg cgctgtttat tcgtgaagtt gtgcgtgaga tgaccacaaa
    29821 agccgggcaa aaatgtacgg caatccggcg gattattgtg ccgcaggcat tggttaatgc
    29881 tgtcagtgat gctctggttg cgcgattaca gaaagtcgtg gtcggtgatc ctgctcagga
    29941 aggcgtgaaa atgggcgcac tggtaaatgc tgagcagcgt gccgatgtgc aggaaaaagt
    30001 gaacatattg ctggctgcag gatgcgagat tcgcctcggt ggtcaggcgg atttatctgc
    30061 tgcgggtgcc ttcttcccgc caaccttatt gtactgtccg cagccggatg aaacaccggc
    30121 ggtacatgca acagaagcct ttggccctgt cgcaacgcta atgccagcac aaaaccagca
    30181 acatgctctg caactggctt gtgcaggcgg cggtagcctt gcgggaacgc tggtgacggc
    30241 tgatccgcaa attgcgcgtc agtttattgc cgacgcggca cgtacgcatg ggcgaattca
    30301 gatcctcaat gaagagtcgg caaaagaatc caccgggcat ggctccccac tgccacaact
    30361 ggtacatggt gggcctggtc gcgcaggagg cggtgaagaa ttaggtggtt tacgagcggt
    30421 gaaacattac atgcagcgaa ccgctgttca gggtagtccg acgatgcttg cagctatcag
    30481 taaacagtgg gtgcgcggtg cgaaagtcga agaagatcgt attcatccgt tccgcaaata
    30541 ttttgaggag ctacaaccag gcgacagcct gttgactccc cgccgcacaa tgacagaggc
    30601 cgatattgtt aactttgctt gcctcagcgg cgatcatttc tatgcacata tggataagat
    30661 tgctgctgcc gaatctattt tcggtgagcg ggtggtgcat gggtattttg tgctttctgc
    30721 ggctgcgggt ctgtttgtcg atgccggtgt tggtccggtc attgctaact acgggctgga
    30781 aagcttgcgt tttatcgaac ccgtaaagcc aggcgatacc atccaggtgc gtctcacctg
    30841 taagcgcaag acgctgaaaa aacagcgtag cgcagaagaa aaaccaacag gtgtggtgga
    30901 atgggcggta gaggtattca atcagcatca aaccccggtg gcgctgtatt caattctgac
    30961 gctggtggcc aggcagcacg gtgattttgt cgattaatcg gtgaatgaag ggcaacggcc
    31021 aatagttgcc cttttatttc actaagtttt gtgacgttgt cacattatgc atgatgtgta
    31081 catctatttt cagggcaccc actgtatgaa aagctggcac acctgccaaa caacctggca
    31141 ggtgcaggca atcccctttg catcagtact gataatgtga acctgactaa accgcccaca
    31201 gagcgcggtt gctaacaaga acacaacatc tgacgaggtt aataatggga agcccctctc
    31261 tgtattctgc ccgtaaaaca accctggcgt tggcagtcgc cttaagtttc gcctggcaag
    31321 cgccggtatt tgcccacggt ggtgaagcgc atatggtgcc aatggatgaa acgcttaaag
    31381 aatttggtgc cgatgtgcag tgggacgact acgcccagat ctttaccctg attaaagatg
    31441 gcgcgtacgt gaaagtgaag cctggtgcgc aaacagcaat tgttaatggt caacctctgg
    31501 cactgcaagt gccggtagtg atgaaagaca ataaggcctg ggtttctgac acctttatta
    31561 acgatgtttt ccagtccggt ctggatcaaa cctttcaggt agaaaagcgc cctcacccac
    31621 ttaatgcgct aactgcggac gaaattaaac aggccgttga aattgttaaa gcttccgcgg
    31681 acttcaaacc caatacccgt tttactgaga tctccctgct accgccagat aaagaagctg
    31741 tctgggcgtt tgcgctggaa aacaaaccgg ttgaccagcc acgcaaagcc gacgtcatta
    31801 tgctcgacgg caaacatatc atcgaagcgg tggtggatct gcaaaacaac aaactgctct
    31861 cctggcaacc cattaaagac gcccacggta tggtgttgct ggatgatttc gccagtgtgc
    31921 agaacattat taacaacagt gaagaatttg ccgctgccgt gaagaaacgc ggtattactg
    31981 atgcgaaaaa agtgattacc acgccgctga ccgtaggtta tttcgatggt aaagatggcc
    32041 tgaaacaaga tgcccggttg ctcaaagtca tcagctatct tgatgtcggt gatggcaact
    32101 actgggcaca tcccatcgaa aacctggtgg cggtcgttga tttagaacag aaaaaaatcg
    32161 ttaagattga agaaggtccg gtagttccgg tgccaatgac cgcacgccca tttgatggcc
    32221 gtgaccgcgt tgctccggca gttaagccta tgcaaatcat tgagcctgaa ggtaaaaatt
    32281 acaccattac tggcgatatg attcactggc ggaactggga ttttcacctc agcatgaact
    32341 ctcgcgtcgg gccgatgatc tccaccgtga cttataacga caatggcacc aaacgcaaag
    32401 tcatgtacga aggttctctc ggcggcatga ttgtgcctta cggtgatcct gatattggct
    32461 ggtactttaa agcgtatctg gactctggtg actacggtat gggcacgcta acctcaccaa
    32521 ttgctcgtgg taaagatgcc ccgtctaacg cagtgctcct taatgaaacc atcgccgact
    32581 acactggcgt gccgatggag atccctcgcg ctatcgcggt atttgaacgt tatgccgggc
    32641 ctgagtataa gcatcaggaa atgggccagc ccaacgtcag taccgaacgc cgggagttag
    32701 tggtgcgctg gatcagtaca gtgggtaact atgactacat ttttgactgg atcttccatg
    32761 aaaacggcac tattggcatt gatgccggtg ctacgggcat cgaagcggtg aaaggtgtta
    32821 aagcgaaaac catgcacgat gagacggcga aagatgacac gcgctacggc acgcttatcg
    32881 atcacaatat cgtgggtact acacaccaac atatttataa tttccgcctc gatctggatg
    32941 tagatggcga gaataacagc ctggtggcga tggacccagt ggtaaaaccg aatactgccg
    33001 gtggcccacg caccagtacc atgcaagtta atcagtacaa catcggcaat gaacaggatg
    33061 ccgcgcagaa gtttgatccg ggcacgattc gtctgttgag taacccgaat aaagagaacc
    33121 gcatgggcaa tccagtttcc tatcaaatta ttccttatgc aggtggtact cacccggtag
    33181 caaaaggtgc ccagttcgcg ccggacgagt ggatctatca tcgtttaagc tttatggaca
    33241 agcagctttg ggtaacgcgt tatcatcctg gcgagcgttt cccggaaggc aaatatccga
    33301 accgttctac tcatgacacc ggtcttggac aatacagtaa agataacgag tcgctggaca
    33361 acaccgacgc cgttgtctgg atgaccaccg gcaccacaca tgtggcccgc gccgaagagt
    33421 ggccgattat gccgaccgaa tgggtacata ctctgcttaa accatggaac ttctttgacg
    33481 aaacgccaac gctaggggcg ctgaagaaag ataagtgatt gtttcagaca aaaaaacggc
    33541 accaggtgcc gttttttact tatgagcgaa ccagattaat accgtacaca caccgactta
    33601 gtttcacacc aaccgtccag ccagtcgggg ccaaaatcac ggcccgttcc tgactgcttc
    33661 atcccaccaa acggtaagtt agcgtcaatt aaggtatggc tgtttaccca caccgtccct
    33721 gcctgtaagc gatcgctata ttccagagcc tgggagagat tttgcgtcca gacactggca
    33781 gttaagccat attccgtgtc gtttgccagt tgtaacgcct cttctccatc cgctactcgc
    33841 accaggttta ccaccggacc aaacacctct tcacgagtta agcgcaattt agcatcggga
    33901 tttaccacca gcgttggcgc aacataatac ccctctccgg ctggtccatt cgacccgcga
    33961 atcagctctg cttgctgtgc ctgcgcatcg tcgaggaatg aacacacttt gtcgcagtgc
    34021 gcacgagaaa ccaaagggtt aatctgtgca acaggtgaca tccccggtcc cacttgcaac
    34081 gattttaccg cctgctcaaa tccactaacc agcgtgtcaa acaacggcgc ttcaatataa
    34141 attcgcgaac tggcggcgca tacttgccct tgattcagga agcttccggt catcaagcct
    34201 tcaataaccc attgcggatc agcatctttt aatacaattg ccgggttttt accgcccagt
    34261 tccagcgtta cacgcgttaa gtgatcagca gcagttctgg caataccttt tcccgtcgcg
    34321 gttgaaccgg taaaactgat tttcgcaaca tgaggatgtg acgtcagggc cgcgccgcat
    34381 acagcacctg acccggtgac gacattaaaa acgccatcag ggataccagc ctcgctggcc
    34441 agttccgcca cgcgcaacat cgtcagtggc gtggtttccg aaggcttaat cacgattgaa
    34501 cagcctgctg ccagtgctgg catcaccttc cacataccaa tcatcaacgg aaagttccat
    34561 ggcacaattc ccgccactac gccaaccggc tctttacgcg tccaggcctg ataacgcgcc
    34621 ccctggggta agggaatcga caagtccagc gttttacccg cgattttggt cgttaacccg
    34681 gcggtataac gcatccagtt cagcgtacag cccacttcaa aagcacgaga aatggcaatt
    34741 gacttgcctt gctccagggt ttccagttgc gccagctcct cactgtgctg ctccaccaga
    34801 tcagcaaaac gtagcagaat acgttcacgc tctgcgggta atcgcccggc ccagcgacgc
    34861 gagacaaagg cccgccaggc agacatgact gcgttatcta catccgcttc gttggcatca
    34921 gcagtagacg caatttcttg cccggtggcc ggatcaaaga tcgccaatcg tttttcactt
    34981 tgtgcggggc ctggacgacc atcaatataa agaccgtgtt gacgatcgag aaactgttgg
    35041 acctggctta atactgctac atgcggctct gtcatcactt ttccttatta tttacccagt
    35101 gtgatttcag tgtacgcagc gacaagaaaa gtcacttttt tcgctgtgac attcgctttg
    35161 tcagctgtga caagctccgc aaatcgtgac aataaccgct tatgtctcta tgcctgagaa
    35221 gacttatttc ctatagtcag gctcattagc ttcgtttact gcaacacaaa tgcaacaata
    35281 aaaatacatt tcacagagcg aaaacgtgcc atgaaccccg cagtggataa tgagtttcag
    35341 caatggcttt cccaaatcaa tcaggtatgc ggaaatttta ccggacacct gctgactgag
    35401 cgttacacgg gtgtactgga tacgcatttt gccaaaggac taaagctgag taccgtgaca
    35461 accagcgggg tgaatttatc ccgcacctgg caggaagtaa aaggcagcga cgatgcctgg
    35521 ttttacaccg tttttcagct tagtggtcag gcaataatgg agcaggatga gcgtcaggtg
    35581 cagattggcg ctggcgatat tacgttactc gatgcctcac gcccctgttc gctttactgg
    35641 caggagtctt ctaaacagat ttcattactt ttgccacgca ctctgctgga acaatatttt
    35701 ccccatcaaa aacctgtctg cgcagaaaga ctggacgctg acttacccat ggtgcaactc
    35761 agtcatcgcc tgttacagga gagcatgaat aatccggcac tttctgaaac agaaagtgaa
    35821 gctgcgctac aggcgatggt gtgtctgctg cgcccggtac ttcatcagcg ggaatctgtt
    35881 caacctcgtc gtgaacgtca gtttcaaaaa gtggttacgt tgatagacga taatattcgc
    35941 gaagagatat tacgcccgga gtggatagcc ggagagacag gtatgtcagt acgtagtttg
    36001 taccgaatgt ttgccgataa aggtttggta gtcgcgcaat atattcgtaa ccgtcgtctc
    36061 gatttttgtg cagatgcgat tcgccatgcc gcagatgatg aaaaactggc aggcatcggc
    36121 tttcattggg gattttctga ccagagtcat ttttcaacgg tatttaagca acgctttggg
    36181 atgacgccag gcgagtatcg acgtaaattc cgctaactaa ttggcgttgt gtgtactgca
    36241 acgccaatta gttaaaacag atatttgact atgcattttt cagggcgaaa ggcaatttga
    36301 caggagtttt tgaatattca ccttgtttca ggaaaataga tcaatcgctt tctcctgtag
    36361 cgttatcccg atattgcggt tttgctatta aaattttcgc acccatttac cgtttatccc
    36421 ttgtacatat tcccccggtt tggcccgagc tacgagcttt tgcccggcga gtttcgcgat
    36481 atcatctaca gacacattat tttgctttgc cagttgttgg taactcgctt tacgttcggc
    36541 attaatgtcg gttactaatt tctctgtttc agcatccgtt ttcagcgcaa ccagataacc
    36601 ataaaatgtt tcacctaccc gcccctgagt tctggcttca tccagagtca atgccattac
    36661 attgctgctt accagcccaa caagaaacgc acaaaaaagt aatgttttct tcatcatcgc
    36721 ctcagaaaag atcgctacga gtttcaagca gctcttcgac atctttgtct gccttgatga
    36781 tgatctcatg ctcaatttta acgttcatat tgatagtgat cggttccttg ggcgcagcga
    36841 cttcaatacg cggcgtacag gcagccagca taaaagatga cgtcaacgca gccagtaaaa
    36901 ttttcattgt ttttcctcac actcttttcc ttgcggacag tcatttcccg gcagacgtac
    36961 gttctgctcc aaccatgcct ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa
    37021 aatattctct tcatgatggt aattaaggtt taccgtaccg cttttaccat caacgcgact
    37081 ggtacctgta atgttggcct gcatggttaa taaaccgaga ttatctaagt taatttttgt
    37141 cgatgaccgg ctaatttcca tatagcgcaa ccagttaatt gctgaacccg cagtcatatt
    37201 gtctttcacc accgcatctg cggtgtcttt atccagccgc aaggtcattg gcccgctatt
    37261 tgccagccaa ccatctttca ctatccattt ttcgctgttt agccataacg gcaatgcacc
    37321 actaaatgcc ccggacatgg cgaattgttt cggattgacg gcgctaacca gttcgctgga
    37381 tgaaagatta ttcagccgca acagcgccgg gtcatgttgc ggcatacgta attgtttcat
    37441 cagtacgtta ccgcctaaca catcgacgct aacatcagtc aacagcaagg gttcttcttc
    37501 agtccacgga taacgccctt gcaaatcagc cgtaatattt ttcgctgtca ccagattaat
    37561 cacttcggca attcgcaacg taacggggcc gcgagtcccc agatgccagg ctccatcggc
    37621 aaaacggaaa ggcaggacaa aatcgacacc gttaacctga ttatctggca tccaggcact
    37681 accgcctttc aacacgccgt gtcctcccgc gcggaatcct tgttcaggcg cagcagaaaa
    37741 tgcaacctga gcatacagtt caccatcgcg taagttcatc ttccagtcgg gtggcaccag
    37801 cggctggaat acagtcagtg attgttttgg ccaccaggcg ttaccgcgca gacgaatacc
    37861 gtcccagcga ccatttaccc gaaccgggcc aatctcacca gcatgtaaat cgcctttaaa
    37921 gaggaaatac gtcggatcgc gcccatcgac gctaaatttt aaggttgatg gcggtaacac
    37981 actgccgcca gtgaacagcg tttgcccggc gtccagtgac agcgcgccgc tgaaggaggg
    38041 atgctgtgcg tcacgtaccc agacgacggg cttgtcgaga attaatcgcg gcttttctac
    38101 cgtcatcgta ccgtattgta actggtcgaa accggtggag aggtcggtca gcgtaatcgt
    38161 gctgtcatgc cattcacctt ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact
    38221 gccttttccc cagtagcgcc actgccagcg gcccgcgtca gggagaaaat cattcgccag
    38281 cccatccata tgcagcacga aatcgcccag ttcattttca tgcgcctgca aaatggcctg
    38341 caaacgtccg tcaacaccac gttgggtgac ttttacaccc gctaaaggcc agcggatttc
    38401 atcgatgtcc agcgaatcga tgactcttcc cttcgaacgt aataacgcgc ctggctcaaa
    38461 ggccagcgtt gggtcagtca gacttccact tagctgcgcg ggtaagcggg catataaaat
    38521 gaggtccgcc tgtttcgctt caccggtcag ttgcagaggc agctggctgt tatccatgct
    38581 taattttcct gggccaaaat ttagcaccgc gttgccttta cccgcttgcc cctgggtcag
    38641 cacactcagt cgtccgctga tcagagcgtt ctcaagccct gcctgccagt tgtcgacttt
    38701 gacacccagt cggccactca aaggaaaacc tgcatacggc caactccagc gaccatcgct
    38761 tacggtcaat tgttgacgag taatttgcca cggcaaatcg agcaacggat cgccgttatc
    38821 ccgtgccagc acaatcaatt gcccgctatt ttcctgccag tccagctctg catccaccag
    38881 tgacggttcc tgcggcaagt ttaacgtcgc agtagcatgc ccacttacag gaagtccatc
    38941 cggcacgagc ggcatagtaa attcccccac cagtttaacc ggcggctgat tttcaaacgc
    39001 gacgacatcc agttcgctga ctgtaagttg ttgccctttc agctggcctt gaaatttaac
    39061 tttttcgccc tgataacgca gttgctggat atcagaggtt aatgcgagag agagttttcc
    39121 ctgccattcc tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcag
    39181 catggactgc cactgcgcga gggtttttgg agcagctggc gattgctccg tctgcggcaa
    39241 tttcgccaga caagcagaat caagttctac cgtgccgacg ttcaataacc agcggctggg
    39301 atgtgaaagg ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag
    39361 atcgggaatg attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt
    39421 cccgagcggt agccagatgc ccgccagagt gggaacccac agcccgagcg tcatcaacag
    39481 cgtcaacggc acaagaataa tcagtaataa cagcgcgaga acggctttat atttacccag
    39541 catgggtagt taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc
    39601 atgtttaacc gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt
    39661 caatatcgcc atagctttca attaaatttg aaattttgta aaatattttt agtagcttaa
    39721 atgtgattca acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag
    39781 tacgacaaga agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt
    39841 gactttctgc tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt
    39901 ttcgtaaacg atgacggcag ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa
    39961 tatatcgccc tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg
    40021 gggctgaaag tagtccgtgt tccagcctat gatccagagg ccgttgctga acacgccatc
    40081 ggtatgatga tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct
    40141 aacttctctc tggaaggtct gaccggattt actatgtatg gcaaaacggc aggcgttatc
    40201 ggtaccggta aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg
    40261 ctggcgttcg atccgtatcc aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat
    40321 ctgccaaccc tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa
    40381 aactaccatc tgttgaacga agccgccttc gatcagatga aaaatggcgt gatgatcgtc
    40441 aataccagtc gcggtgcatt gattgattct caggcagcaa ttgaagcgct gaaaaatcag
    40501 aaaattggtt cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt ctttgaagac
    40561 aaatccaacg acgtgatcca ggatgacgtg ttccgtcgtc tgtctgcctg ccacaacgtg
    40621 ctgtttaccg ggcaccaggc attcctgaca gcagaagctc tgaccagtat ttctcagact
    40681 acgctgcaaa acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga actggtttaa
    40741 tcttgccgct cccctgcaat ccaggggagc tgattcagat aatccccaat gacctttcat
    40801 tctctattct taaaatagtc ctgagtcaga aactgtaatt gagaaccaca atgaagaaac
    40861 tagccgcgtt tgttgcgcta agcctgctga tggcgggatg tgtaagtaat gacaaaattg
    40921 ctgttacgcc agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc
    40981 ccgtgaccag cgataaaaat ccgccagaaa tcagctttgg tgaaaaaatg atgatttccg
    41041 gcagcatgtg taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgacagcca
    41101 aagggctggc aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca
    41161 ccattagcga aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac cagttaacgc
    41221 tggcgaccgc gaaacagaca ttaacttata agctggcgga tttaatgaat taatagctgc
    41281 cacagctccc ggcggcaagt gactgttcgc tacagcgttt gccgttgggt aatgcacaca
    41341 tcccaatcgc cgtaccatcc agttgacggg caacagaaag cgaaccgccg atcattgcac
    41401 aatttgcttc tccactactg gacatcgacg cttttaaacc tggcgctacg tgcgccgcag
    41461 tcgcctgctg aacaggttca ctactacacg ccgacaacaa taaagcggca caccctaccc
    41521 aaaacgctgc tcgcatctct tcttcctctg atcttcaagc caaacgacac cgccataaat
    41581 aataggcagc acagagggcg gcgtcgagag ctgtcctgcg cgttgccccg catttttact
    41641 tttttatggc tatttttttg ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg
    41701 gacacaaaag cgaaaatgca gaagaaagcc atttgctaaa attgaaggat tactactggg
    41761 cgcgcagcaa tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata tgattactat
    41821 tgacggtaat ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta tcgccatcta
    41881 ccctattacc cccagttcca cgatggcaga acaggctgat gcctgggccg gaaacggctt
    41941 gaagaacgtt tggggagaca caccacgcgt ggttgaaatg cagtcggaag cgggtgctat
    42001 cgctaccgtg catggcgctt tgcagacggg tgccctttca acatcgttta cgtcatcgca
    42061 gggtttgctg ctgatgatcc cgacgctgta caaactggca ggcgaactca caccgtttgt
    42121 cctgcatgta gcggcacgta ccgttgccac acatgcactc tctatttttg gcgatcattc
    42181 cgacgtcatg gcggtgcgcc agacgggttg cgcgatgttg tgtgcggcaa acgtccagga
    42241 agcgcaagac tttgctctca tttcgcacat cgcgacgctg aaaagccgcg tgccatttat
    42301 tcatttcttt gatggtttcc gcacgtccca cgaaatcaac aaaattgtcc cgctggccga
    42361 tgacacgatt cttgatctca tgccgcaggc cgaaattgat gctcatcgcg cccgggcact
    42421 caacccggaa catccggtga tccgcggtac gtccgccaat cctgacactt atttccagtc
    42481 tcgcgaagcc accaacccat ggtacgacgc ggtctatgac catgttgaac aggcgatgaa
    42541 tgatttctct gccgcgacag gtcgtcagta tcagccgttt gaatattacg ggcatccgca
    42601 agcggaacgg gtgattatcc tgatgggctc tgccattggc acctgtgaag aagtggttga
    42661 tgaattgcta acccgtggcg aaaaagtcgg cgtgctgaaa gttcgcctgt accgcccctt
    42721 ctcagccaaa cacttgctac aaactctacc gggatccgta cgtagcgtgg cggtactgga
    42781 cagaaccaaa gaacccggtg cccaggcaga accgctctat ctggatgtga tgaccgcact
    42841 ggcagaagcc tttaataatg gcaagcgcga aactctcccc cgtgtcattg gtgggcgcta
    42901 tggtctttca tccaaagaat ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa
    42961 cgcggctaaa ccgaaagcgc gctttacggt tggtatttac gatgatgtaa ccaatctgtc
    43021 cctgccgttg ccggaaaaca cccagccaaa ctcggcgaaa cttgaagcct tgttttatgg
    43081 ccttggtagt gatggcagcg tttccgcgac caaaaacaat atcaagatta tcggtaattc
    43141 cacgccgtgg tacgcacagg gctattttgt ttacgactcc aaaaaggcgg gcggcctgac
    43201 ggtttctcac cttcgagtga gcgaacagcc gattcgttcc gcttatctca tttcccaggc
    43261 tgattttgtt ggctgccacc agttgcagtt tatcgataaa tatcagatgg ctgagcgttt
    43321 aaaacctggc ggcattttcc tgctcaacac accgtacagc gcagatgaag tgtggtcgcg
    43381 cttgccgcaa gaagttcagg ccgtgttaaa ccagaaaaaa gcgcgcttct atgtgattaa
    43441 cgcggcgaaa atcgcccgcg aatgtggcct ggcggcccgt attaataccg tcatgcagat
    43501 ggcttttttc catctgacgc aaattctgcc tggcgatagc gccctcgcag aattgcaggg
    43561 tgcgattgcc aaaagttaca gtagcaaagg ccaggatctg gtggaacgca attggcaggc
    43621 tctggcgctg gcgcgtgaat ccgtagaaga agttccgttg caaccggtaa atccgcacag
    43681 cgccaatcga ccgccagtgg tttccgatgc cgctcctgat ttcgtgaaaa ccgtaaccgc
    43741 tgcgatgctc gccgggcttg gtgacgccct ccccgtttcg gcgctgccgc cagacggctc
    43801 ctggccgatg ggcactacgc gctgggaaaa acgtaatatt gccgaagaga tccccacctg
    43861 gaaagaggaa ctctgtaccc aatgtaacca ctgcgttgcc gcttgcccac actcagctat
    43921 tcgcgcaaaa gtggtgccgc ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct
    43981 ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg caccggaaga
    44041 ttgcaccggt tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga
    44101 gattaaagcc atcaatatga tgtctcgcct ggaacatgtc gaagaagaga aaatcaatta
    44161 cgatttcttc ctcaacctgc cagaaatcga ccgtagcaaa ctggaacgta ttgatattcg
    44221 tacatcgcag ctgattacac cgctgtttga atattcaggt gcttgctccg gttgtggcga
    44281 gacgccgtat attaaattgc tgactcagct ctatggcgac cggatgttga tcgctaacgc
    44341 caccggctgt tcttcaattt atggcggtaa cctgccctct acaccgtata ccaccgatgc
    44401 caacggtcgt gggccggcat gggcgaactc tctatttgaa gataatgccg aatttggcct
    44461 tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc tggatcaatt
    44521 tgccgataaa atcccggcgg aattactgac ggcgttgaaa tcagacgcta cgccagaggt
    44581 tcgtcgagaa caggtcgcag ctttacgcca gcaactcaac gatgttgccg aagcacatga
    44641 actgctgcgt gacgcagatg cactggtgga aaaatcaatc tggctgattg gtggcgacgg
    44701 ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga cggaaaacgt
    44761 caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg cgtcgaaagc
    44821 gacaccgctg ggtgcagtaa ctaagttcgg cgagcacggc aaacgtaaag cgcgtaaaga
    44881 tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc
    44941 gcagctgaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg ggccatcgct
    45001 gatcattgct tatagcccgt gtgaagagca tggttacgat ctggcactca gccatgacca
    45061 gatgcgccaa ctcacagcta ccggcttctg gccgctatat cgcttcgatc ctcgtcgtgc
    45121 cgatgaaggc aaactgccgc tggcgctgga ttcacgcccg ccgtcagaag cactggaaga
    45181 aacgttactt cacgagcaac gtttccgtcg tctgaattcg cagcagccag aagtggcaga
    45241 acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc tggcacaaat
    45301 ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgctc tggataagga ttatccaatt
    45361 ctaaaaaaaa agcccggacg actgttcggg cttgtctttt tatatgttgg aaaatcagtg
    45421 gcaatgcaat ggcccaacag aaaataaaat ctgtgaca
//
LOCUS       DAZFRJ010000013        45311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000013 DAZFRJ010000000
VERSION     DAZFRJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.013"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..1454
                     /gene="yajR"
                     /locus_tag="ACHKUN_000670"
     CDS             90..1454
                     /gene="yajR"
                     /locus_tag="ACHKUN_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6658983.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1582..2073)
                     /gene="yajQ"
                     /locus_tag="ACHKUN_000671"
     CDS             complement(1582..2073)
                     /gene="yajQ"
                     /locus_tag="ACHKUN_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO6658984.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2241..3152
                     /gene="panE"
                     /locus_tag="ACHKUN_000672"
     CDS             2241..3152
                     /gene="panE"
                     /locus_tag="ACHKUN_000672"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO6658985.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3115..3705
                     /gene="yajL"
                     /locus_tag="ACHKUN_000673"
     CDS             3115..3705
                     /gene="yajL"
                     /locus_tag="ACHKUN_000673"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO6658986.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3759..5207)
                     /gene="thiI"
                     /locus_tag="ACHKUN_000674"
     CDS             complement(3759..5207)
                     /gene="thiI"
                     /locus_tag="ACHKUN_000674"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO6658987.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5413..5655
                     /gene="xseB"
                     /locus_tag="ACHKUN_000675"
     CDS             5413..5655
                     /gene="xseB"
                     /locus_tag="ACHKUN_000675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO6658988.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5655..6554
                     /gene="ispA"
                     /locus_tag="ACHKUN_000676"
     CDS             5655..6554
                     /gene="ispA"
                     /locus_tag="ACHKUN_000676"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO6658989.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6579..8441
                     /gene="dxs"
                     /locus_tag="ACHKUN_000677"
     CDS             6579..8441
                     /gene="dxs"
                     /locus_tag="ACHKUN_000677"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO6658990.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8621..9595
                     /gene="yajO"
                     /locus_tag="ACHKUN_000678"
     CDS             8621..9595
                     /gene="yajO"
                     /locus_tag="ACHKUN_000678"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO6658991.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9649..10167)
                     /gene="pgpA"
                     /locus_tag="ACHKUN_000679"
     CDS             complement(9649..10167)
                     /gene="pgpA"
                     /locus_tag="ACHKUN_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO6658992.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10145..11122)
                     /gene="thiL"
                     /locus_tag="ACHKUN_000680"
     CDS             complement(10145..11122)
                     /gene="thiL"
                     /locus_tag="ACHKUN_000680"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO6658993.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11200..11619)
                     /gene="nusB"
                     /locus_tag="ACHKUN_000681"
     CDS             complement(11200..11619)
                     /gene="nusB"
                     /locus_tag="ACHKUN_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO6658994.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11639..12109)
                     /gene="ribE"
                     /locus_tag="ACHKUN_000682"
     CDS             complement(11639..12109)
                     /gene="ribE"
                     /locus_tag="ACHKUN_000682"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO6658995.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12198..13301)
                     /gene="ribD"
                     /locus_tag="ACHKUN_000683"
     CDS             complement(12198..13301)
                     /gene="ribD"
                     /locus_tag="ACHKUN_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO6658996.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13305..13754)
                     /gene="nrdR"
                     /locus_tag="ACHKUN_000684"
     CDS             complement(13305..13754)
                     /gene="nrdR"
                     /locus_tag="ACHKUN_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO6658997.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13905..14444
                     /gene="yajI"
                     /locus_tag="ACHKUN_000685"
     CDS             13905..14444
                     /gene="yajI"
                     /locus_tag="ACHKUN_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6658998.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14743..15627
                     /gene="tsx"
                     /locus_tag="ACHKUN_000686"
     CDS             14743..15627
                     /gene="tsx"
                     /locus_tag="ACHKUN_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6658999.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15804..16151)
                     /gene="yajD"
                     /locus_tag="ACHKUN_000687"
     CDS             complement(15804..16151)
                     /gene="yajD"
                     /locus_tag="ACHKUN_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO6659000.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16280..17251)
                     /gene="secF"
                     /locus_tag="ACHKUN_000688"
     CDS             complement(16280..17251)
                     /gene="secF"
                     /locus_tag="ACHKUN_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO6659001.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17262..19109)
                     /gene="secD"
                     /locus_tag="ACHKUN_000689"
     CDS             complement(17262..19109)
                     /gene="secD"
                     /locus_tag="ACHKUN_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO6659002.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19137..19469)
                     /gene="yajC"
                     /locus_tag="ACHKUN_000690"
     CDS             complement(19137..19469)
                     /gene="yajC"
                     /locus_tag="ACHKUN_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO6659003.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19492..20619)
                     /gene="tgt"
                     /locus_tag="ACHKUN_000691"
     CDS             complement(19492..20619)
                     /gene="tgt"
                     /locus_tag="ACHKUN_000691"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO6659004.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20675..21745)
                     /gene="queA"
                     /locus_tag="ACHKUN_000692"
     CDS             complement(20675..21745)
                     /gene="queA"
                     /locus_tag="ACHKUN_000692"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO6659005.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21838..22419
                     /gene="acpH"
                     /locus_tag="ACHKUN_000693"
     CDS             21838..22419
                     /gene="acpH"
                     /locus_tag="ACHKUN_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO6659006.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22424..24241)
                     /gene="malZ"
                     /locus_tag="ACHKUN_000694"
     CDS             complement(22424..24241)
                     /gene="malZ"
                     /locus_tag="ACHKUN_000694"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO6659007.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24397..25770)
                     /gene="proY"
                     /locus_tag="ACHKUN_000695"
     CDS             complement(24397..25770)
                     /gene="proY"
                     /locus_tag="ACHKUN_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO6659008.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25846..27165)
                     /gene="brnQ"
                     /locus_tag="ACHKUN_000696"
     CDS             complement(25846..27165)
                     /gene="brnQ"
                     /locus_tag="ACHKUN_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO6659009.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27572..28867)
                     /gene="phoR"
                     /locus_tag="ACHKUN_000697"
     CDS             complement(27572..28867)
                     /gene="phoR"
                     /locus_tag="ACHKUN_000697"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO6659010.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28925..29614)
                     /gene="phoB"
                     /locus_tag="ACHKUN_000698"
     CDS             complement(28925..29614)
                     /gene="phoB"
                     /locus_tag="ACHKUN_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO6659011.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29804..31006
                     /gene="sbcD"
                     /locus_tag="ACHKUN_000699"
     CDS             29804..31006
                     /gene="sbcD"
                     /locus_tag="ACHKUN_000699"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO6659012.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31003..34146
                     /gene="sbcC"
                     /locus_tag="ACHKUN_000700"
     CDS             31003..34146
                     /gene="sbcC"
                     /locus_tag="ACHKUN_000700"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO6659013.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETQEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34272..35456
                     /gene="araJ"
                     /locus_tag="ACHKUN_000701"
     CDS             34272..35456
                     /gene="araJ"
                     /locus_tag="ACHKUN_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO6659014.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35599..36507)
                     /gene="mak"
                     /locus_tag="ACHKUN_000702"
     CDS             complement(35599..36507)
                     /gene="mak"
                     /locus_tag="ACHKUN_000702"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO6659015.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36632..37543
                     /gene="rdgC"
                     /locus_tag="ACHKUN_000703"
     CDS             36632..37543
                     /gene="rdgC"
                     /locus_tag="ACHKUN_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO6659016.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37621..37704)
                     /locus_tag="ACHKUN_000704"
     CDS             complement(37621..37704)
                     /locus_tag="ACHKUN_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659017.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37701..38042)
                     /locus_tag="ACHKUN_000705"
                     /pseudo
     CDS             complement(37701..38042)
                     /locus_tag="ACHKUN_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38190..38474)
                     /gene="ppnP"
                     /locus_tag="ACHKUN_000706"
     CDS             complement(38190..38474)
                     /gene="ppnP"
                     /locus_tag="ACHKUN_000706"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO6659018.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38546..39223)
                     /gene="aroM"
                     /locus_tag="ACHKUN_000707"
     CDS             complement(38546..39223)
                     /gene="aroM"
                     /locus_tag="ACHKUN_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO6659019.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39481..39672)
                     /gene="yaiA"
                     /locus_tag="ACHKUN_000708"
     CDS             complement(39481..39672)
                     /gene="yaiA"
                     /locus_tag="ACHKUN_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO6659020.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39722..40246)
                     /gene="aroL"
                     /locus_tag="ACHKUN_000709"
     CDS             complement(39722..40246)
                     /gene="aroL"
                     /locus_tag="ACHKUN_000709"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO6659021.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40429..40887)
                     /gene="yaiI"
                     /locus_tag="ACHKUN_000710"
     CDS             complement(40429..40887)
                     /gene="yaiI"
                     /locus_tag="ACHKUN_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO6659022.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41007..41816
                     /gene="proC"
                     /locus_tag="ACHKUN_000711"
     CDS             41007..41816
                     /gene="proC"
                     /locus_tag="ACHKUN_000711"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO6659023.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41833..42948)
                     /gene="adrA"
                     /locus_tag="ACHKUN_000712"
                     /gene_synonym="dgcC"
     CDS             complement(41833..42948)
                     /gene="adrA"
                     /locus_tag="ACHKUN_000712"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO6659024.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43050..43370)
                     /gene="psiF"
                     /locus_tag="ACHKUN_000713"
     CDS             complement(43050..43370)
                     /gene="psiF"
                     /locus_tag="ACHKUN_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO6659025.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43489..44904)
                     /gene="phoA"
                     /locus_tag="ACHKUN_000714"
     CDS             complement(43489..44904)
                     /gene="phoA"
                     /locus_tag="ACHKUN_000714"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO6659026.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45005..45265)
                     /gene="iraP"
                     /locus_tag="ACHKUN_000715"
     CDS             complement(45005..45265)
                     /gene="iraP"
                     /locus_tag="ACHKUN_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO6659027.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        11151 a        12157 c        11436 g        10567 t
ORIGIN      
        1 tctcctctgt aatattttct cattaacact ctgtttaccc aacatgcgct ccgcactaca
       61 ctatgtgggc atttttttaa ggggtggtaa tgaacgatta taaaatgacg ccaggtgaga
      121 ggcgcgcgac ctggggttta gggaccgtat tctcgttgcg catgctgggc atgtttatgg
      181 ttctgccggt tctgaccacg tacggcatgg ctctgcaagg tgccagcgaa gcattaatcg
      241 gtattgccat tggtatttat ggtctgactc aggccgtttt tcagattccg tttggcctgc
      301 tttccgaccg cattggtcgc aaaccattaa ttgtcggtgg gctggcggtg tttgccgccg
      361 gtagcgttat cgctgcgctt tccgactcca tctggggaat tattctgggc cgggcgctac
      421 aaggctctgg agcaattgcc gccgccgtta tggcgctgct ttccgatctc acgcgcgaac
      481 aaaaccgcac caaagcgatg gcgtttatcg gcgtgagctt tggcattacc tttgccattg
      541 cgatggtgct tggcccgatc atcactcaca aacttgggct gcacgcgctg ttctggatga
      601 tcgctattct ggcaacaacc ggcattgcgt tgaccatttg ggttgtaccc aacagtagca
      661 ctcacgtact taatcgtgag tccggaatgg tgaaaggcag tttcagtaaa gtgctggcgg
      721 aaccgcggct gctaaaactc aactttggca ttatgtgtct gcacatgctt ctgatgtcta
      781 cgtttgttgc cctgcccgga cagctagctg atgcggggtt cccggcggct gaacactgga
      841 aggtctatct ggcgacgatg ttaatcgcct ttggctcggt cgtgcctttc attatctacg
      901 ctgaagttaa gcgcaaaatg aagcaagtct ttgtcttctg cgtcgggttg atcgtggttg
      961 cggaaattgt gttgtggaac gcccaaacgc agttctggca actggtggtc ggcgtgcagc
     1021 ttttctttgt ggcgtttaat ttgatggaag cccttctgcc ctcacttatc agtaaagagt
     1081 cgccagcagg ttacaaaggt acggcgatgg gtgtctactc caccagccag tttcttggcg
     1141 tggcgattgg cggttcgctg ggcggctgga ttgacggcat gtttgacggt cagggggtat
     1201 ttctcgctgg cgcaatgctg gccgcagtgt ggctggcagt cgccagtacc atgaaagaac
     1261 cgccgtatgt cagcagtttg cgcattgaaa tcccggcgga cattgccgca aacgaagcgt
     1321 taaaagtgcg tttgctggaa actgaaggcg tcaaagaagt gttgattgca gaagaagaac
     1381 attcagctta tgtgaaaatc gacagcaaag tgacgaatcg ctttgagata gaacaggcaa
     1441 ttcgtcaggc ataaaaaacg cggcgaattg cctgctgcgc tacgcttatc aggcctacaa
     1501 agtttctgaa ttacaaacct ttgtaggccg aatcaggcgt tcatgccgca tctgacatga
     1561 acaaaacgca catagtcgcg attaatcgcg gaagtttttg aactggaacg gctgaccgag
     1621 atcgccacca cgtaccatcg ccatgacagc ctgcaaatca tcacgagatt tgcccgttac
     1681 gcggatttca tcgccctgaa tttgcgcctg cactttcagt ttgctgtctt tgatcatctt
     1741 gacgattttc ttctgggtcg cgctctcaat gccctgtttc agtttcgctt ccacaaacca
     1801 ggttttaccg ctatgaacga tattctccgg tacatccagc gaactgcctt caatgccgcg
     1861 cttcagcagc ttggcacgca gaatatccag caactgattg acctggaaat cggactcgct
     1921 caacactttg atggttttgc tggcgtcgtt cagctcaaat gaggcttcaa cgttacggaa
     1981 gtcaaaacgg gactccactt cgcggctcgc gttatcgacc gcgttacgtg cttcctgaag
     2041 atcaacttca gagacaatat cgaaagatgg catcttttcc tctcccttca tttttgatgc
     2101 gaagcataat acccgcaaag ttaaataacc gacagtcctg caccaggacg tatcccttaa
     2161 ttgtgcatag tcgcaacagc atgcacaatt aagggatagc cttattgaac acccggagtg
     2221 gttgcgggtg aggaggaaca atgaaaatta ccgtattggg atgcggtgcc ttagggcaat
     2281 tatggcttac agcactttgc aaacagggtc atgaagttca gggctggctg cgcgtaccgc
     2341 aaccttattg tagcgtgaat ctggttgaga cagatggttc gatatttaac gaatcgctga
     2401 ccgccaacga tcccgatttt cttgccacca gcgatctgct cctggtgacg ctgaaagcat
     2461 ggcaggttta cgatgccgtc aaaagcctcg cgtccacact gcctgtaacc acgccaatac
     2521 tgttaattca caacggcatg ggcaccatcg aagagttgca aaacattcag cagccattac
     2581 tgatgggcac caccacccat gccgcccgcc gcgacggcaa tgtcattatt catgtggcaa
     2641 acggaatcac gcatattggt ccggcacggc aacaggatgg cgattacagt tatctggcgg
     2701 atattttgca aaccgtgttg cctgacgttg cctggcataa caatattcgc gccgagctgt
     2761 ggcgcaagct ggccgtcaac tgtgtgatta atccactgac cgccatctgg aattgcccga
     2821 atggtgaatt acgtcatcat ccgcaagaaa ttatgcagat atgcgaagaa gtcgcggcag
     2881 tgatcgagcg cgaagggcat catacttcag cagaagattt gcgtgattac gtgatgcagg
     2941 tgattgatgc cacagcggaa aatatctcgt cgatgttgca ggatatccgc gcgctgcgcc
     3001 acaccgaaat cgactatatc aatggttttc tcttacgccg cgcccgcgcg catgggattg
     3061 ccgtaccgga aaacacccgc ctgtttgaaa tggtaaaaag aaaggagagt gaatatgagc
     3121 gcatcggcac tggtttgcct cgcccctggt agtgaagaga ctgaagccgt caccactatc
     3181 gatctgctgg ttcgcggcgg tatcaaagtc accactgcca gcgtcgccag cgatggcaac
     3241 ctggccatta cctgctcgcg cggcgtgaag ctgctggcgg atgcgccgct ggtcgaggtg
     3301 gctgatggcg aatatgacgt gatcgtactg cctggtggca ttaaaggcgc agagtgtttt
     3361 cgcgatagta ccctgctggt tgaaaccgtt aaacagttcc accgttccgg gcgtatcgtc
     3421 gcggctattt gcgccgcgcc agccaccgtg ctggtgccgc acgatatctt cccgattggt
     3481 aatatgaccg gcttcccgac gctgaaagac aaaattcccg ctgaacaatg gcaggacaag
     3541 cgcgtcgtct gggatgcacg ggtaaaattg ctgaccagcc aggggccggg gacagctatc
     3601 gactttggtc tgaaaattat cgacctgctg gttgggcgcg agaaagccca tgaagtggca
     3661 tcacaactgg tgatggcggc agggatttat aattattacg agtagtgtcg gatgcggtaa
     3721 gcgtcgcatc tgaccagatg cgacgttaca accacaaatt acgggcgata taccttcaca
     3781 ttgttaaagc cctgctcgcg cagatagagc gcctgcagac ggctcatcac cccgcgctca
     3841 caccacagta gccaggttct gttctggtcg agatcgccaa acttggtgct cagtttatag
     3901 aacggcagag aaactacatc aatcccttcg actttcagtg gcttatcttc ctgttcatcg
     3961 atagaacgga tatcgaggat cacgtcgttc gggccgaagc cattgacggt ttccacttcc
     4021 accacttcct gctcggtctg ctgggcgatt tcgcggatat caacgttatt cgcttcttca
     4081 accactttat cgagaatgct gaagtcgaat ttctcttctt ccgcttcaat cttcgactta
     4141 accgctttca ccgtcgggct tttggagata acaccgcaat attccggcat cgtgcgagca
     4201 aagtcttcgg tgccaatctg gcgagccagg ttgatgatgt gctctttgtc gtaagaaatc
     4261 agcggacgca ggatcagcgt atcggagacg ttatcaatca ggcgcaggtt ggtcagcgtc
     4321 tgactggaca cctggccgag cgcttcgccg gtgaccagcg cctgtacgcc gtaacgttca
     4381 gccactttag acgcggcacg caccatcata cgtttgagga taacgcccat ctgaccgtcg
     4441 tcgattttct cgagaatttc cccgacgacc ggttcgaaat taatagcgac aaaacgcacg
     4501 cggtgggagc tgccaaaacg gttccacaga taatgcgcca cctgacgcac gccaatttca
     4561 tgcgccgcgc cgccaaggtt aaagaaacag taatgcacgc ggcagccgcg acgcatcaac
     4621 atataactgg aaacaccgga gtcgaaacca ccggaaatga gcgacagcac atcttcctgg
     4681 gtgccgatcg ggaaaccgcc aataccttcg tagcggcctt taatcagcag gagacgatcg
     4741 tcttccactt ccagatggac agtcacttcc ggattggtca gcttcacgcg cgcggactca
     4801 atatgctgat ttaaaccgcc gccgacgtaa cgttccacat caatcgagct aaaatcatgt
     4861 ttgccacggc gcttcacgcg tacgcagaag gttttgcctt ccagctgatc gcgatactga
     4921 accaacgctt tctcaaaaat atcgtgcatg tcggtaaacg gcacgtcttc gacttcgaga
     4981 atatggtgga tacccggaat acgggtcagg gcgtcgcgaa tagccagacg ctggttttca
     5041 tcttttgcgc gaacttcgat gttatcccag tggcggacga cagcgagcgt ctcatcatag
     5101 tgctttaaaa cgttacgaat gttcccggta aggattttta taaagcgcaa gcgcacagat
     5161 tggcttttga tggtgatttc cgggaacaat ttaatgataa acttcatggc ggcaatggtt
     5221 cgttggcaag ccttaagcga cttgtatagg gaaaaataca gcagcccaca cctgcggctg
     5281 catccaggcg cggaagtata ccactaacat cgctttgctg cgcacatcac cttaccattg
     5341 cgcgttattt gctatttgcc ctgagtccgt taccatgacg gggcgaaaaa tattgagagt
     5401 cagacattca ttatgccgaa gaaaaatgag gctcccgcca gctttgaaaa ggcgctgagc
     5461 gagctggaac agattgtaac ccgtctggaa agtggcgacc tgccgctgga agaggcgctg
     5521 aacgagttcg aacgcggcgt gcagctggca cgtcaggggc aggccaaatt acaacaagcc
     5581 gaacagcgcg tacaaattct gctgtctgac aatgaagacg cctctctaac cccttttaca
     5641 ccggacaatg agtaatggac tttccgcagc aactcgaagc ctgcgttaag caggccaacc
     5701 aggcgctgag ccgttttatc gccccactgc cctttcagaa cactcccgtg gtcgaaacca
     5761 tgcagtatgg cgcattatta ggtggtaagc gtctgcgacc cttcctggtt tatgccaccg
     5821 gtcatatgtt tggcgttagc acaaacacgc tggacgcacc cgctgccgcc gtagagtgta
     5881 tccacgctta ctcattaatt catgatgatt taccggcgat ggatgatgac gatctgcgtc
     5941 gcggtttgcc gacctgccat gtgaagtttg gcgaagcaaa cgcgattctc gctggcgacg
     6001 ctttacaaac gctggcgttc tcgattctaa gcgatgccaa tatgccggaa gtgtcggatc
     6061 gcgacagaat ttcgatgatt tctgaactgg cgagcgccag cggtattgcc ggaatgtgcg
     6121 gtggtcaggc attagattta gacgcggaag gcaaacacgt acctctggac gcgcttgagc
     6181 gtattcatcg tcataaaacc ggcgcattga ttcgcgccgc cgttcgcctt ggtgcattaa
     6241 gcgccggaga taaagggcgt cgtgctctgc cagtactcga caagtacgca gagagcatcg
     6301 gccttgcctt ccaggttcag gatgacatcc tggatgtggt aggagatact gcaacgttgg
     6361 gaaaacgcca gggtgccgac cagcaacttg gtaaaagtac ctaccctgca cttctgggtc
     6421 ttgagcaagc ccggaagaaa gcccgggatc tgatcgacga tgcccgtcag tcgctgaaac
     6481 aactggctga acaatcactc gatacctcgg cactggaagc gctagcggac tacatcatcc
     6541 agcgtaataa ataaacaata agtattaata ggcccctgat gagttttgat attgccaaat
     6601 acccgaccct ggcactggtc gactccaccc aggagttacg actgttgccg aaagagagtt
     6661 taccgaaact ctgcgacgaa ctgcgccgct atttactcga cagcgtgagc cgttccagcg
     6721 ggcacttcgc ctccgggctg ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca
     6781 acaccccgtt tgaccaattg atttgggatg tggggcatca ggcttatccg cataaaattt
     6841 tgaccggacg ccgcgacaaa atcggcacca tccgtcagaa aggcggcctg cacccgttcc
     6901 cgtggcgcgg cgaaagcgaa tatgacgtat taagcgtcgg gcattcatca acctccatca
     6961 gtgccggaat tggtattgcg gttgctgccg agaaagaagg caaaaatcgc cgtaccgtct
     7021 gtgtaatcgg cgatggcgcg attaccgcag gcatggcgtt tgaagcgatg aatcacgcgg
     7081 gcgatatccg tcctgatatg ctggtggtcc tcaacgacaa cgaaatgtcg atttccgaaa
     7141 atgtcggcgc gctcaacaac catctggcgc agctgctttc cggtaagctt tactcttcac
     7201 tgcgcgaagg cgggaaaaaa gttttctctg gcgttccgcc aattaaagag ctgctcaaac
     7261 gtaccgaaga acatattaaa ggcatggtag tgcctggcac gttgtttgaa gagctgggct
     7321 ttaactacat cggcccggtt gacggtcacg atgtgctggg gcttatcacc acgctgaaga
     7381 acatgcgcga cctgaaaggc ccgcagttcc tgcatatcat gaccaaaaaa ggtcgtggtt
     7441 atgaaccggc agaaaaagac cccatcactt tccacgccgt gcctaaattt gatccctcca
     7501 gcggttgttt gccgaaaagt agcggcggtt tgccgagcta ttcaaaaatc tttggcgact
     7561 ggttgtgcga aacggcagcg aaagacaaca agctgatggc gattactccg gcgatgcgtg
     7621 aaggttccgg catggtcgag ttttcacgta aattcccgga tcgttacttc gacgtggcaa
     7681 tcgccgagca acacgcggtg acctttgccg ccggtctggc gattggtggg tacaaaccca
     7741 ttgtcgcgat ttactccact ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg
     7801 cgattcaaaa gcttccggtc ctgttcgcca tcgaccgcgc gggcattgtt ggtgctgacg
     7861 gtcaaaccca tcagggcgct tttgacctct cttacctgcg ctgcataccg gaaatggtca
     7921 ttatgacccc gagcgatgaa aacgaatgtc gccagatgct ctataccggc tatcactata
     7981 acgacggccc gtccgcggtg cgctacccgc gcggtaacgc ggttggcgtg gaactgacgc
     8041 cgctggaaaa actgccaatt ggcaaaggca ttgtgaagcg tcgtggcgag aaactggcga
     8101 tccttaactt tggtacgctg atgccagacg cggcgaaagt cgctgaatcg ctgaacgcta
     8161 cgctggtcga tatgcgtttt gtgaaaccgc ttgatgaagc gttaattctg gaaatggccg
     8221 ccagccatga agcgctggtc accgtagaag aaaacgccat tatgggcggc gcaggcagcg
     8281 gcgtgaacga agtgctaatg gcccatcgta aaccagtacc cgtgctgaac attggcctgc
     8341 ctgacttctt tattccacaa ggaactcagg aagaaatgcg cgccgaactc ggcctcgatg
     8401 ccgccggtat ggaagccaaa atcaaggcct ggctggcata atccctactc cactcctgct
     8461 atgcttaaga aattattcat agactctaaa taattcgagt tgcaggaagg cggcaaacga
     8521 gtgaagcccc aggagcttac ataagtaagt gactggggtg aacgaatgca gccgcagcac
     8581 atgcaacttg aagtatgacg agtatagcgg gagtggcagc atgcaataca accccttagg
     8641 aaaaaccgac cttcgcgttt cccgactttg cctcggctgt atgacctttg gcgagccaga
     8701 tcgcggtaat cacgcatgga cactgccgga agagagcagc cgtcccatca ttaaacgcgc
     8761 gctggaaggc ggcataaatt tctttgatac cgccaatagc tattccgatg gcagcagcga
     8821 agagatcgtt ggtcgcgcac tgcgggattt cgcccgtcgt gaagacgtgg tcgttgcgac
     8881 gaaagtgttc catcgcgttg gtgatttacc ggaaggatta tcccgtgcac aaattttgcg
     8941 ctctatcgac gacagcctgc gccgtctcgg catggattat gtcgatatcc tgcaaattca
     9001 tcgctgggat tacaacacgc cgatcgaaga gacgctggaa gccctgaacg acgtggtaaa
     9061 agccgggaaa gcgcgttata tcggcgcgtc atccatgcac gcttcgcagt ttgctcaggc
     9121 cctgaagcta caaaaacagc acggctgggc gcagtttgtc agtatgcagg atcactacaa
     9181 tctgatttat cgcgaagaag agagcgagat gctgccactg tgttatcagg agggcgtggc
     9241 ggtgattccg tggagcccgc tggcgcgggg gcgactgacg cgtccgtggg gagaaactac
     9301 cgcacgactg gtgtctgatg aggtggggaa aaatctctat aaagaaagcg atgaaaatga
     9361 cgcgcagatc gcagagcggt taacaggcgt cagcgaagaa ctcggtgcaa cacgagcaca
     9421 agttgcgctg gcctggttgt tgagtaaacc gggcattgcc gcaccgatta tcggaacttc
     9481 gcgcgaagaa cagcttgatg agctattgaa cgcggtggat atcactttga agccggaaca
     9541 gattgccgaa ctggaaacgc cgtataaacc gcatgcggtc gtaggattta aataagagtc
     9601 caggcctgat gagacgtgac aagcgtcaca tcaggcatcg gtgcacaact acgacagaat
     9661 acccagcggc cagtgatgac cgataaagta caggatgcct gcggaaatca ccccggcgac
     9721 aatatcgtcg atcatgatcc ccatgccgcc atgtacattg cgatcaaacc agcggatcgg
     9781 ccacggcttc cacatatcca gaatacggaa aatcacaaac ccggcggcaa cccactgcca
     9841 gtcattggtc ggcagcgcca tgagcgtgat ccacatacca ataaattcgt cccagacaat
     9901 gctgccgtga tcgtgcacgc ccatatcttt cgccgtttga tgacaaagat agacgccgat
     9961 acagatcccc aacatcacca ccagcgagta gagctgccag ggtaaaaagg tcatcagata
    10021 ccagaacgga atcgctgcca gcgatcccat cgtgccagga acgattgggc ttaatccact
    10081 tccgaatccg acagcaagca gatgccacgg attactcatc ttcaggcgac ttttcgcgac
    10141 atctttatgg cgtggcaaaa tggtcatatc ctttccagtc aaacgtgaca ggttcgccgt
    10201 cacgaataaa acaaagccct tcgatatcgg cggtcatttg cccgatacag gtaaacggta
    10261 cgcccaggtg tccgagagcc acatccagcg cgccacggtt cagttccggc acagtgaaac
    10321 acaactcgta atcttcaccg ccagagagcg cccagcgcag cgcctgttcc ggttcaacat
    10381 ggcgagaaag cgcatcagaa aacggcagca atgccaggtc aatacgtgcg ccgcagtcgc
    10441 tggctttcac gatatgcccg agatcggaaa tcagaccgtc agagagatcg atagctgaat
    10501 ttgccagatc gcgcagtgcc tgcccctgta aaatacgcgg cgatggacgg agatgacgtt
    10561 tgatcaagta gtcagcatct ttagcatcgg caacctgcaa acggttttgc aaaatcgcca
    10621 gcccggcggc gctatcgccc ggtgtaccgg tcacatagat ccagtcaccc ggtttcgccc
    10681 cagagcgcgt taaggctcgt cccatcggaa caaagccgtg gatacccaac gtcattgata
    10741 atggcccacg cgtggtatcg ccgccaatga gttgcatatc gtaataattg agaagatcaa
    10801 acaaactgtc gctgaaggac tcaagccacg cttcgtctac gtccggtaag gttaatgcca
    10861 gcgtcagcca ggccggatcg gcccccatcg ctgccagatc gcttaggttc actgccagtg
    10921 ctttatacgc cagatcagca ggatcgatat catggaggaa gtggttgccc gccaccagcg
    10981 tatcagtgct gatcgccagg gtctgtttct cggggatatt gagaagagcg caatcgtcgc
    11041 cgatgcccag ttcgacatca agacgagaac ttcttacacg gtcaaaataa cgggcaatca
    11101 gggagaactc gccacatgcc atacgttatg cctcagcagg taaaaaagaa aaggccggcg
    11161 atcgcggaac ggtcttccgt gaatctaccg gcctggatat cactttttgt tagggcgaat
    11221 cacaggtgct gctttatcga gtacgccgtt gacgaactta tggctgtctt ctgcgccgaa
    11281 cgatttcgcc agttcgatcg cttcgttaat ggccactttg tatggcacat cgctacgttt
    11341 agacagctcg tacagcgcaa tgcgcagtac tgctttttct acctgtccca gttcttccag
    11401 caggcgggac aggtatggct tcatcagtcc gtcgaggtat gcggtgttag tcgccacccc
    11461 ggccagcagc tcacggaagt acaggacgtc aacgtctttt acatcctgtt cagccaggaa
    11521 ctggtattca acatcagcga tgtcgttctg ggacaactgc caggagtaga gcgcctggac
    11581 ggcacactca cgagcgcggc gacgagcagc aggtttcacg gatttcccct tactaatttc
    11641 aggccttgat ggctttcaat acattaatca tttcaagcgc ggtcagtgca gcttctgcac
    11701 ctttgttgcc agctttggtg ccagcacgtt cgatcgcttg ttcaatgctt tcagtggtca
    11761 gaaccccaaa agcaaccgga atttcgctgt cctgggcaac atgcgccagg ccgttgcttg
    11821 caccaccagc gacatattca aagtgggcag tgccaccacg aataaccgta cccagcgcaa
    11881 tcaccgcgtc gtatttaccg gttttagcca gtgcacccgc cgccagcggc agctcatagg
    11941 caccaggcac ccaaacaacg gtaatgtttt catcttttac ctgaccgata cgtttcagtg
    12001 cgtcaattgc accttccagc aggctgtcat tgataaagtt gttgaaacgc gcgatggtga
    12061 tggcgacgcg agcgtccggg gtagcaacgt tagcttcaat aatgttcatt ttcttccttc
    12121 gggttcgagt atggccccgc aggggggcgg attttagcat aatatttcgt gcgctgcttc
    12181 cctttcgagc cgggagatca tgcacccact aaatgcaggc aaacatccgg gcctacatga
    12241 cgtatctctt tgaatttaaa ttggggggcg tcggctaatt tctcaagccc tggcagcgtg
    12301 cataatccgc gggcgtcgct gcctaatagt ttaggtgcga tatagacaat cagctcatcg
    12361 actaaacccg cctgcagcaa tgcgccagcg agcgttggcc ccgcttccac ccagatactg
    12421 ttaatttgct gtttacccag ttgcatcatc agtacaacca gatccagatg acctttatgc
    12481 tctggaatca gcaaggtacg caccgtttcc ggccactcac tagaatcttc ctgcgtacgc
    12541 gcgaaccagg tttcgccggg ctgctgcaca atgcgatgtt ccggcgtcac gcgattttgg
    12601 ctatcaatca caatacgtac tggctggcgg agattttgtt gcgggtagag cgcctgagtt
    12661 tgttcatcca gttcagacca acgcactgtt aaggctggat catccgccag caccgtggcg
    12721 ctgctagtta aaatggcatg actttgtgcg cgctgtcgtt gtacgtcgcg ccgcgcctga
    12781 ggcgaagtga tccactggct ttcgccgctc gccatcgccg tgcggccatc aagcgatgcg
    12841 ccaagtttta actgaatata aggaaagccg gtgcgcatcc gcttgagaaa gcctttattc
    12901 aattgctcgg cttcactcat catcagcccg tggctgacgt caatgccagc ctgttgcaga
    12961 cggtaaagtc cacgcccagc gacctgcggg ttaggatctt gcatcgaggc aaccacgcgc
    13021 gctacgccag cggcgatgag tgcgtcacag cacggtggcg tacgaccatg atggctacag
    13081 ggttcgagtg tgacataggc ggtcgcacct ttggcttttt cacccgccat acgcaacgcg
    13141 tgtacttcgg catgtggttc acccgcacgt tggtggtaac cttcaccgac aatttcgcca
    13201 tctttgacaa tgacgcaccc gacattcggg ttgggatgcg tggtaaaacg tcctcgttgc
    13261 gccagcttta gcgctcgcgc catgtaatac tcgtcctgca cggcttagtc ctccaggcgc
    13321 gcgatctctt cgccaaattc tttgatatct tcgaaactgc ggtagacaga ggcaaaacgg
    13381 atataggcga ctttatcgag ctttttcaat tgctccatca ccagattgcc aatcatcttg
    13441 ctcggcactt cgcgctcacc ggtggcgcgc agctgcgatt taatatgatt gattgccatt
    13501 tcgacgtcat cggaactcac cggacgtttt tccagcgccc gcagcattcc gctacgcaat
    13561 ttctcttcat taaacggttc acgcacgtcg ttgcttttta caacacgcgg cataaccagc
    13621 tccgccactt caaaggtggt gaaacgttca ttacacacca gacactgccg acggcggcgt
    13681 acggatgaac cctcgcccac gagacgagag tcaattacct tagtgtccac ggcgaaacag
    13741 aatgggcaat gcatacggcg tcctgaccag gtggttaaca gaaatctatt ttaccctgaa
    13801 ctgacgatac aacaaaggcg caacgctttt gagataaagg atcgatgctt tcgctgcttt
    13861 taccctctac cattaggcta attgcgacga gaaagggacg acctatgaat actaacgttt
    13921 ttcgactgct cctgctggga agcctgttca gccttagcgc ctgtgtgcag cagagcgaag
    13981 tgcgacagat gaaacacagc gtcagcacgc tgaaccaaga gatgacgcaa ctcaataaag
    14041 aaacggtcaa aatcactcag caaaacaggc tgaatgcaaa atccagcagt ggggtttacc
    14101 ttctgccagg agcgaaaaca ccggcaagac tggaaagcca gatcggtact ttacgtatgt
    14161 cgctggtgaa tattacgcct gatgcagatg gcaccaccct gacgctacgt attcagggcg
    14221 agtcgaatga tcccttgccc gcattcagcg gaactgttga atatgggcaa attcagggaa
    14281 caatagacaa ctttcaggaa atcaatgtgc agaatcaatt gattaatgcc cctgccagcg
    14341 tcctcgcccc cagcgatgtt gatattccgt tacagttaaa gggtatttct gtagaacagt
    14401 tagacttcgt acgtatccac gatattcaac ctgttatgca gtaaacgtat ttcggggcga
    14461 ttttgtgcgt ctcgcaacat ctttccccgt cattttgtta ctccgcttac atcaccctga
    14521 ttgatagtaa aagtttgcaa caagggtgaa agtcagtaca atccctgccc gaatgtgtgt
    14581 aaacgtgaac gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg
    14641 agcaatgatt tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc
    14701 ctttttcact cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg
    14761 gcagccggtg cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac
    14821 aaaccgcagt atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac
    14881 acccgtttcg gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa
    14941 aaagactggt tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc
    15001 gatgctaaag gtatctggaa ccacggttct ccgctgttta tggaaatcga accacgtttc
    15061 tccatcgaca agctgaccaa tactgacctt agcttcggtc cgttcaaaga gtggtacttc
    15121 gcgaacaact acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac
    15181 atgggtctgg gtaccgacat cgatactggt ctgccgatga gcctgtccat gaacgtctat
    15241 gcgaaatacc agtggcagaa ctatggcgca gcgaacgaaa acgagtggga cggttaccgt
    15301 ttcaaaatta aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc
    15361 ggcttcacca acttcgactg gggttccgat ttaggggatg acagcggtaa cgcaatcaac
    15421 ggtattaaga cccgtactaa taactctatc gcttccagcc atattctggc tctgaactac
    15481 gatcactggc actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac
    15541 gatgcagaac tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt
    15601 tacctggttg taggttacaa cttctgatta tgaaaatgcc gggatttatt cccggcattt
    15661 cagattgtta acaacgtgcg ctttgttcat gccggatgcg gcgtaaacgc tttgtccgac
    15721 ccacaaaagc acgtaaattc aatatattgc tgaaataacg tagtcctgat aagcgtagcg
    15781 catcaggcaa ttttacgttt taatcacttc ttcttgttca tcatcgcttt caggtcagcg
    15841 aatgggttgt acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc
    15901 gtaccatact gatccgcttc ggtatatttc gaatgctcat gatcgtggca atagagacaa
    15961 agcaattccc agttactgcc atcttccggg ttattggtat ggtcgtgatc aatgtggtga
    16021 acagtaagtt cacgcaggtt ggaataaaca aactcgcggg aacagcgacc gcagacccac
    16081 ggatatattt ttaatgcttt ttcgcgatag ccgctttcta accgcgcata gttttttggg
    16141 atgatagcca tgaagagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg
    16201 cggggatttt cgcaatctcc atacatcaaa aaaaatcccg atcttctgac cgggattttc
    16261 aacatcaacg ggaacttgat tacggcagaa ttgacggctg atccgcccct tctttttcca
    16321 ctttctgctg caacatgtgt tcgcgcttca tacccagttt cagagccaac gcagatgcca
    16381 catagatgga agatgcagta ccgatggaaa caccgataag catggtcagc gagaagcctt
    16441 ccagtaccgg accaccgaag aggtacagca tcaggataac catcaaggta gtaccggatg
    16501 tgatcaaggt acggtgcagc gtctgggtca aggacacgtt aaagatttcg taaggcgtac
    16561 cgcgacggat cttgcggaag ttttcacgaa tacggtccga taccacgata ctgtcgttaa
    16621 gcgagtaacc gataaccgac atcaacgatg ccacaatggt caggtcaatc tcgatatgga
    16681 ataacgacaa aatacccagc gtaatgataa cgtcgtgcgc cagcgcaata accacccctg
    16741 ccgccagtcg ccactcaaag cgaaaaccta cgtacacaag gatagacagc agcgccgcca
    16801 tcaacgccat cgcaccggtt tgcgcaaggt ctgcccccac gctcggaccg acgaactcaa
    16861 tacgcttcac tgctgcattc tgattggtgg attcgttaat caccttcaga acctggctgc
    16921 ccagcacctg accgccggtt tcgccttcag caggcggcat acggaccatg atgtcgtggc
    16981 tgctaccaaa gttttgcagc attggctctt caaaaccggc tttttgcaat gcatcacgca
    17041 ttacgtcaat ttcagccggt ttttcgagcg taatttcgat aaccgtacca ccggtgaaat
    17101 ccagccccca gttaaagccg cgcacgccca taataacgat agcggcgatt aacagcagac
    17161 cagagatgcc gaaagcccag tagtcccagc gcataaagtc atagacttta cggccgtggt
    17221 ttagttgttc aacagtatat tcctgtgcca catcgcactc ctcagattga cagcttcttg
    17281 acgcgcttgc cgccatatag caggtttacg atggcacgcg taccgacaat cgcggtaaac
    17341 atcgacgtcg ccacaccgat accggtagta atcgcgaacc ctttaattgc cccggtaccc
    17401 actgcgtaca ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa gatagaactg
    17461 aatgcgccac gataaccttc atcaattgcc tgttgaacag tacgtccgtt gctcaactct
    17521 tctttaatac gttcgttgat cagtacgttc gcatcgaccg ccaccgcaag ggttaagacg
    17581 atacccgcga tacctggcat actcagcgtt gcgcctggca acagcgacat aatgccgacg
    17641 attaagatca agttggcaat cagagcactg gtcgcaatca gaccaaactt cttatagaag
    17701 atgatcatga acagaataga aaccagcaga ccggcaaggc aagcctccag cccctgttca
    17761 atgttctgca tacccagggt tgggccaatg gtacgttctt caacaatctg aatcggcgcg
    17821 atcaacgcac cggcacgcag cagcagtgac agctgacggg cttcgttcgg gttgttgatg
    17881 ccggtgatac ggaagctgtt acccagacga gactggatgt tggcgatgtt aatcacctct
    17941 tcctgtttca ccagaaccgc acgaccattt gcatctttct taccgctgtc tttgtactcc
    18001 acaaacaggg ttgccatcgg tttgccgatg ttgtccttag tgaagttaga catgatgtta
    18061 ccaccagcgc tatcgagcga gatgttaacc tgcggttggt tgtattcgtc ctggctggaa
    18121 gtggagtcgg tgatatggtc accggtcaga attacgcgtt tgtacagcac aactggctga
    18181 ccttcgcggg tctgtttcac ttcagagtcg cccggtacgc gaccggatgc cgcagcggcc
    18241 tggtcaacgt tggtgtttac cagacggaat tccagcgttg ccgtcgcacc cagaatctct
    18301 ttcgcacgcg cagtgtcctg aatacctggc agttcaacaa cgatacggtc agcaccctga
    18361 cgctgaacca ccggctccgc cacgccaagt tggtttacac ggttacgcag gatattgatg
    18421 ttctgctgca ccgcatattc acgcgcttca ctcagacgag catcgctcat taccgcacgc
    18481 agctggttgc tgccctggct actaatcacc aggtccggat ggcgcttgct cagatacgca
    18541 atggcttcat cacgagcttt agcatcgcgg aaagtaatgc tcaggccgta gttgttttct
    18601 ttacgaacgg tggtatacgg gatgcccttt tcacgcagat cgctgcgcag gctatcgata
    18661 ttttgttcct ggagtttgcc aagcgcggtg tccatatcca cttccatcag gaagtgaacg
    18721 ccgccacgca ggtcaaggcc gagcttcatc ggctcagcgt gaatagctgc cagccagcgc
    18781 ggcgttgccg gggcaaggtt aagcgccacg acgtatttgt cacccataac gcccattaat
    18841 gcttcacgag cgcgcagctg ggtgtcagtg gagtcgaagc gcgcaagaat agcgccctct
    18901 tccagtgcca cagacttagc agttattttt tcttcttgta acgttttctg gacctggatc
    18961 agcgtttgct cactggcggc gactccgcgc gcaccagtga tctgaacagc cggatcctca
    19021 ccaaacaggt tgggaagcgc atacagcaga ccgatgacaa tcaccacgat cagcatgacg
    19081 tacttccaca aaggataacg gtttaacacg gcaattccct tagggaaaaa ttttaattac
    19141 agcgccttca tggtgccttt cggcaggacg gcagctacga agtcacgttt aataactact
    19201 tcagtggtgt cattcagcgc gatagcaatg tagccgtttt ccgctacttt ggttacgcga
    19261 ccaaccaggc caccgttcgt cagaacttca tcacctttgg caatggagtc catcagcttt
    19321 ttgtgttctt tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa aatcagaccg
    19381 aataccacca gcatcaaaat caaagacatc gggctacctt gcgccggtgc acccgttgcc
    19441 gctaccgcat cagaaataaa aaagctcatt aaatttccct cattattaat attaatcaac
    19501 gttcaaaggt ggtacttctc gcccctgacg ctggtaaaaa tcagttacga agctctctaa
    19561 tttaccctct tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt aacgaaggtt
    19621 atgaatggtg ttgagtcgcg cgcctaatat ttcgttgcaa cggtcaagat gatgcaagta
    19681 agcgcgtgaa taattgcgac aggtgtagca atcacactca ggatcgagtg ggccagtatc
    19741 gctcttatac ttcgcattgc ggattttcac cacgccatcg gtcacgaaca aatgaccatt
    19801 tcgggcgttg cgggttggca ttacgcagtc aaacatatcg ataccacgac gtacgccttc
    19861 aaccaggtct tctggtttac caacgcccat caggtaacgc ggtttgtctg ccggaatttg
    19921 cgggcataca tgctccagaa tgcggtgcat atctgctttc ggctcaccca cagccagacc
    19981 gccgacagcg tagccatcaa aaccgatatc taccagacct ttaacagaaa tatcacgtaa
    20041 atcttcgtaa acgctgccct gaatgatacc aaacagcgca tttttgtttc cgagactgtc
    20101 aaaacgctca cggctacgct tcgcccaacg cagagacatc tccatggagc gttttgcgta
    20161 atcccagtca gcaggatacg gcgtacactc atcaaagatc atgacgatat ccgaaccaag
    20221 atcgtactga atctccattg atttttcagg atcgaggaaa atcggatcgc cgttgatcgg
    20281 gttacggaag tgcacgccct gttcggtgat tttacgaata tcgccaaggc tgaagacctg
    20341 gaagccgccg gagtcggtga ggatcggccc cttccactgc ataaaatcgt gcagatcgcc
    20401 gtgcagtttc atgatttcct ggcccgggcg cagccacagg tggaaggtgt tgccgaggat
    20461 aatttgcgcg ccagtggctt caacttcttc cggcgtcatc ccttttacgg tgccgtaggt
    20521 gccaacaggc ataaaacaag gcgtttccac tacgccacaa tcaaagacca ggcggccacg
    20581 gcgtgcgcga ccgtcggtgg tgtccagttc aaatttcatt ttttctccac tacgtcagaa
    20641 aaacagtcca acgttttaaa ccagcgccgc ggaattactc cccgacgcgc tcattaattg
    20701 cctgcggatt gtacgtgata aacatcgcat caccgtaact aaaaaagcga tatttctctt
    20761 ctaccgctgc tttataggcg ttcatggtgt gttgataacc ggcaaaggcc gaaaccagca
    20821 taatcagcgt cgattccggc aggtggaagt ttgtcaccag cgcatcgacc actttgtact
    20881 ggaagcccgg atagataaag atttgggtat cgtcaaagaa cggttcgata agatcgtttt
    20941 tcgccgcctg agccgcgctt tccagcgaac gtactgaggt ggtgccaacc gcaatcaccc
    21001 ggttaccgcg cgctttcgcc gccagtaccg cgtctaccac atcctgcggc acttcagcgt
    21061 attccgagtg catgatgtga tcttcaatgg tgtcgacgcg caccggctgg aaggtgcccg
    21121 caccaacgtg caacgtcaca aacgccatct ccacgccttt ggcgcgtaat ttttccagca
    21181 aaggttcgtc aaaatgcaga cctgcggtcg gggctgcaac cgcgcccggt ttttcgctat
    21241 aaacggtttg ataaagttcg cggtcagcgt cttcgtccgg acggtcgata tacggcggca
    21301 gcggcatatg gccgatgctg ttgagaatat ccagcaccga gcgttcatca ttaaattcga
    21361 cttcaaacag tgcgccgtgg cgcgcggtca ttgttgcgtt aatactttcg tcatcgccca
    21421 gcagcagttc tgcgccaggt tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt
    21481 tgtcgtcgag catccgttca accagcactt caatcttgcc gccgctggct ttacgcccaa
    21541 acaggcgcgc cgggatcacg cgggtattat taaaaaccag aagatcgccg gggttgagct
    21601 tatcaagtaa atcggtgaaa gtaccgtgcg tcagcgcgcc cgtcggcccg tccagcgaca
    21661 gtaaacgaca gctactgcgt tcaggcatgg gatagtgggc aatcagggat tcgggcaatt
    21721 caaaggagaa atcggtaacg cgcatgacac tgactcagac taaaataaga ggcgggtagt
    21781 ctagtgccgg ggcgctctcc ctgcaacctt tacccccttt tggatagaat ataatcgatg
    21841 aattttttag ctcacctgca tttagcccat ctcgcggaaa gctcgctttc cggcaattta
    21901 ctggctgatt tcgtacgcgg aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc
    21961 attcatatgc atcgacgtat cgacgtatta accgacaatc tgccggaagt ccgcgaagca
    22021 cgggaatggt ttcgtagtga aactcgccgc gttgcgccta ttacgctgga tgtcatgtgg
    22081 gatcactttc tttcccgcca ctggtcgcag ctgtcgccgg actttccgct acaggaattt
    22141 gtctgttatg cccgcgagca agtgatgacg attttgccgg actcaccgcc acgttttatc
    22201 aatctgaaca attacttgtg gtcagagcag tggctggtgc gctatcgcga tatggatttc
    22261 atccagaacg tgttaaacgg catggcaagc cgccgcccac gtctggatgc cctgcgtgac
    22321 tcctggtacg atttagacgc tcattatgac gccctcgaaa cccgcttctg gcagttttat
    22381 ccgcggatga tggcgcaggc gtcacacaag gcgttataaa acgttagttc atccataccg
    22441 tagccgaaat ggcaggcaaa gccagaatcc cgtcagtcag ttgcccatgc ccttctttgc
    22501 attgccattg cacggcattg agcaacggtg acgcgggtag caccacttca caagcttcgc
    22561 cacggttgat tgccaccagt acacgctgct gattcagcac gcggacaaat accaccacgt
    22621 tatcttccgc atacagcacc tgacagccgc catgacgtag cgcctgactt ttcttacgca
    22681 gcgcaatcat tcgctggtac agcgcgaata acgccgtatc ctgcttttcc acctgccagg
    22741 ggaacggttt acggcaaaac ggatcgtttt tgccatccag tcctacttca tcaccgtaat
    22801 aaatgcacgg tacaccaggc caggtgaaca gccagaccac cgccagcggc aggcgcgcaa
    22861 tatcccgacc gagcagcgtt ttaaatcgcg cagtatcgtg gctgtcgagc tgattaaaca
    22921 tacgtaattg ttgttgatga gaaagccctg cgcggtaatt atccatccag gccatacagg
    22981 tttgggcatc aatttgctgc ggatcgtaag agatatcggt attggcaaga aatccccaca
    23041 acgggaatgt gaagccacga tagttcatgg cggcatcttc cacatcggcc tgtaaccatt
    23101 gccgtgcatc gccaaaatgt tcgccgacaa tatacgcttc cggctgggtt tctttcgccg
    23161 cttcggtgat cccagcaacg tgctgcatat tattgcgcgc cccacccgcc tcccccagca
    23221 tatgcaccac atccagccgc cagccgtcca tactccacgg cgctttcagc caatggcgga
    23281 caatactgtc ttccccgcga taaatttcat tcaccagact ttccgactga taatccagct
    23341 tcggcaagct ggcatagcca agccagtcga gcgccgtgcc atcatcacta aacgagtacc
    23401 agtcgcgcca gggcgattca gggttgtgac aagctccccc cgtgccacga ttgtgcctgt
    23461 caaaccaggc atgggaatcg ccactgtggt taaacacgcc atccagcacc agccgcattc
    23521 ccagctgctg cgtattgtgg cgtaagcgca gcaacgcccc atcaccgtca aactgcggat
    23581 cgacatggcg ataatcctcg gtatcgtatt tatgtacgct gggagcttta aacaccggat
    23641 tgagatacag cgccgtcacg ccaagctttt tcagatacgg cagtttttcg cttatcccgt
    23701 ccagatcgcc gccatagaac gttgatccgc ccgcctgcgc cgtgaccggt tcatcccagt
    23761 cacgcaagat gatctcttgt ccggctgcat gatggtaata gacatgatcc tgttcagctt
    23821 cacgaggaag actacgcgca aaacgatcag ggaagatctg ataaaaaatc tgatccgcag
    23881 cccattgtgg gccgatatcc ggtacatcga cggcaaactg ctccagtcgt gccggcggca
    23941 ttcggctgaa gccctgcggt gtaaaccaac gctggcgatc gtgccacagc agtttgaaac
    24001 tgtaacgccg ccggggttgt ccgctggaga gatcaatcgc cgcacgccat gcggtgacgc
    24061 caggctgctg ctgactgcgc tgcttatgca tcgatactga catttcttcg ttatcgtgtt
    24121 ctgtacgcag cataatgcgc tgcggtgggt cttcgcccgt cagccacagt gtaatgagca
    24181 gttgatcttt gctttgttta acaaatgggg gcaccggcag gtgccatgca tttaacatca
    24241 taattcccct gtgatgaaaa tctgatcacc ttgccatagc ctaatgcagg atcactcatc
    24301 atctgaagga tatttggggg aggaggacgg gggcggataa ctttcggaat agccggatca
    24361 ggatttcctc ctgaaccggc ttacagaacg caagtattat tggttttcag ccagctgacg
    24421 atcgtggcgg cgtttaaaca tccagccaat caacagcaca acaatccacg cgaaaccgac
    24481 atacagcgag atacgcgtat ccgggtgata accaatcaac ccgataataa agagcaggaa
    24541 aatcagcccg ccgatggtcg ttgctacccc acccggcact ttaaatttca gcgccttaac
    24601 ttcttctggc ggcaaacggc gacggaaggc aatttgcgac agcaggatca taatccacac
    24661 ccacaccgtg gcgaaggttg ccagcgaagc gatcaccagg aagacgtttt ccggcatgat
    24721 gtagttcaga tacaccgcaa acagcagcgc ggtagtcatc accagcaccg taacccacgg
    24781 aataccgcga cgcgacgttt tgctgaaaat tttcggcgcg ctgccctgct ctgccatacc
    24841 gtggagcata cggcctacgc caaatacgtc actgttaatt gccgacagcg aagcagtcag
    24901 cacaacaaag ttaagaatgc tggcggcaaa ggtaatgccc atatgctgga acgtcagcac
    24961 gaacgggcta ccggcagtgc caacctgatt ccacgggtag atagacataa tgacgaacag
    25021 cgtaccgacg tagaacacca gaatacgcat cggcacggag ttaatcgcac gcggtatcga
    25081 tttctcagga tctttcgctt caccggcggt aatcccgata atttcgatcc caccgtaagc
    25141 aaacatcacc atttgcaacg acatcaccat cccaagccag ccgttactga agaagccgcc
    25201 gttgctccac aggttatgaa taccggtcgg ttgcccgccg ttgccaatcc cccagatgat
    25261 gatgccgaaa ccggcgacaa tcatgatgat gatggtggcg actttaaaga acgagaacca
    25321 gaattccagc tcaccgaaga ccttcacgct catcaggttt acggcgcaaa tgatcagcac
    25381 cacgctcagt acccaaatcc agtgcggcac cgtcgggaac cagacaccca tatagatacc
    25441 aaaagcggtc acatcggcga tggcgacaat aaggatttca aagcaatagg tccagccggt
    25501 aatgtaacct gccagcgggc cgaggttttc ctgcgcataa cgcgagaaag agctggctgc
    25561 cgggttatgt accgacattt cccccagcgc acgcataatg atatacgccg cgataccacc
    25621 gataatatag gccaacaaca cgctcggacc ggccattttg atggcgtctg ccgaaccgta
    25681 aaacagcccg gtgccaattg ctgaacccag tgccataaag cgtatgtggc gggtacttag
    25741 cccacgcttt agcttgttct tactttccat caattcctaa cccatcaaca caataaaaaa
    25801 ccacggggca tcgcagcccc gtggttaaaa caaatgttca gtgatttagt gagcgctgga
    25861 ggtcacctga cgacctgccg cgcgatccca gataatggcc agaaccacca tcaccactgt
    25921 tggcattaac cacgccagac cttgttcggc cagcggtaaa cgctgcgccc aggacggtaa
    25981 gatatcgctg aatgcagatg ctttgatccc gtcgagaata ccaaaaagca ggctgataaa
    26041 catcggcgga gcaatcacgc gggacgaatt atgccaccat gagcgtgtaa aacttaatac
    26101 aaccagtgcg atacacggcg gataaatagc ggtcagcacc ggtacggaga tctggatcag
    26161 ctggcttaag ccgaggttag aaaccaccat cgagaagccg ccgaggataa acaccagcgt
    26221 acgataagag agcggtacgt actgggcaaa gaattctgca caagcacagg tcaggccaac
    26281 tgccgttacc aggcaggcga tgaagattaa cgccgccagc aggaagctac cgccgccgcc
    26341 aaaggtgtgc tgaacgtaag catgcagaat agcagcgccg tttgcagact gatcgaccag
    26401 cgacgcgctg tctgaaccca gacggaacag cgccaggtac agcagagtca gaccaacacc
    26461 cgccatcagg ccagcccaga cggtataacg ggtcagcaga cgcgcttcgg taacgccacg
    26521 agaacgcgcc gcgttaacaa taacgatacc aaacaccatt gcgcccagcg tatccatggt
    26581 cagatagccg ttaacgaagc cgttagaaaa cgcagcgttt tgataagcct cagtcgccgt
    26641 gctgatagaa cccgccggcc agacaatcgc ggcaacagac aggatgacca gcgcgataat
    26701 tttcagcggc gcaaggaagt tgcccacggt atccagcagc ttgcccggat agagcgaaac
    26761 cagaataacg atagcgaaat agaccaggct gtagataaac agcggcagcg cggaatcacc
    26821 cgtcagcggc gcaatcccca cttcaaagga aacggtagct gtacgcggcg tagcgaaaag
    26881 cggccccacc gccaggtaac aaaccgttgc cagcagtacg ccagcgactt taccgattgg
    26941 cgtgctgagg ctgtcaacac cgccgccaac ttttgccagc gccactaccg ttaataccgg
    27001 caggccaacg gcagtaatga ggaagccgaa tgccgcagtc cagacgtgtt cgcctgcctg
    27061 taagccgacc attggaggga aaataatgtt acctgcgccg acgaacaacg caaatgtcat
    27121 aaagcccaga gcgatgatat cgcgcgatct taattgatgg gtcataaatc atactgcctg
    27181 tggatgtggt gttgtaacgt taaatttttg tgccaatccc tggggatgat acagcggtat
    27241 atttaaacaa ttttagcggg aaatactccc aatcaggcgc ggcgaagaat agtttttcga
    27301 tttaccgcgt taatgcagtc agtatgacag cacctgaagc gcaatttaaa cgcttataac
    27361 gttttaaagc aaggagggat ctaaaaacca gaggtatata gcgataagaa atcaccaacc
    27421 agtataaaac actatttccg tgaaaaagac atggctaatc atgcgaacaa aaaatcacca
    27481 accgttgtaa aatctaacgc ccgtattttg actgcaaaga aaataagcca gcatcggctg
    27541 gcttatggca ataaaagatg acaaaggcgg attaatcgct gtttttggca attaaacgtt
    27601 ccgggataac aaaactgaaa cgtgttcctt ttcctactgt actctcaata ttcaggcgac
    27661 tttcgtgatg attcaccgca tgtttcacga tcgctaaccc taatccgcta ccgccggttt
    27721 gccgggaacg cgctttatca acgcgataaa aacgctcggt caggcgcgga atatgctccg
    27781 gtgcaatgcc cggtccgtta tcttcaacgc taaattcggc accgtgcggc actcgcagcc
    27841 agcgtacggt gatatgcgtg ccttccggcg tatgattcac ggcgttatag accaggttcg
    27901 aaatcgcact gcgtagctga tcttcgttgc cagacacctt gaggccgtta tctatctcaa
    27961 aggtaaatgt ctgttttttc tgactcagag tctgagcctc gcgctcaaca acgcgcagca
    28021 tcatcggcac atcaaccttt tcattgagca attgcgtcgg cgcggcttca attttcgaca
    28081 gcgttagcaa ctgcttcacc agcccttcca tccgctgggt ctgctcgcgc atggtgtgca
    28141 acgctttttc gcgtaccgcg ccttccagcg gctgctcatc catcatctcc aggtaaccct
    28201 gtaacacggt caatggcgta cgtaactcat ggctcacgtt ggcaaaaaag ttacgccgcg
    28261 ccccttccag ttgatgcatt tgcgtgacat cacgcgccac catcagcaac tgtttgtggg
    28321 tataaggcat gacgcgaatt tccagatgcc gcccggtgtt gagcaccaga ttgagcgggc
    28381 gagaaaaatc acgcgttttc agatattgcg taaactccgg gtaacgcagc aggttaagga
    28441 tgttctgccc gttatcttcc ggccagcgca aaccaagaat ttgttgcgcc agaccgttac
    28501 accagaaaat accgccctct tccgtggtca gcaccactgc gtcgggcagc gactccgcgc
    28561 cgctacgaaa gcgtttaatc agattgccca gttcacggcg gcgtttttta tttcgcagct
    28621 gcatctggtg taagccgtat agtagcggtt cccagctacc acgccccggt ggcggggtca
    28681 tactacgatc cacccacagc caccatgaaa ggcgcaataa attccagaaa tgccagataa
    28741 gcagtcccgt taccgatgcc agcaaaaacc agggcaggta accaaaaaat gcacccagga
    28801 tgaaagccgg gaggcagcaa agtagcagct ccagcaccag ccttttccac gacagccgtt
    28861 ccagcacgta agatactcca gttaagaaat cataagccct gctctgcgtc cgatgagcaa
    28921 ggcgttaaaa gcgggttgaa aaacgatatc ctgtaccgcg cacggtctgc accatgcggt
    28981 catgcccgcc gggctccagt gctttacgca ggcgacgaat gtggacatcg accgtgcggt
    29041 cttccacata cacgttagtt ccccagacgt ggtttaacag ctgctcgcgg ctgtacacgc
    29101 gctcaggatg cgtcataaaa aagtgcagca gtttaaattc tgtcggcccc atctccagcg
    29161 gctcttcgcc cgccatcact cggtgagatg tcgggtcgag acttaatccc tgcatctcaa
    29221 tcacctcttc caccgccatt ggcgaaatac ggcgcattac cgctttgatt cgcgccacca
    29281 gctccttcgg cgaaaacggc ttggtgatat agtcatccgc gccggtttca aggccgcgca
    29341 cgcgatcttc ttcttcccct ctggcggtca acatcaccac tggaatatcc cgggtcatcg
    29401 actcgcgctt gaggtgtttg atgaactgga taccggagcc gccaggtaac atccagtcaa
    29461 ggagaattaa atccggccag ggttcattca gttgattcac agcactgtca taatcttccg
    29521 cttcgaccgg ctgaaagcca ttttgttcga gcacgaagca gaccatttcg cgaattggag
    29581 cttcatcttc tacgaccaga atacgtctcg ccatgatttg ccctgttgta ataaataggt
    29641 tgcgatcatt aatgcgacgt cattatgcgt cagatttatg acagatttat gaaaagctcg
    29701 tcgcacatat cttcaggtta ttgatttccg tggcgcagaa aaaagcaaat ggcacatctg
    29761 tttgggtata atcgcgccca tgctttttcg ccagggaacc gttatgcgca tccttcacac
    29821 ctcagactgg catctcggcc agaacttcta cagtaaaagc cgcgaagctg aacatcaggc
    29881 ttttcttgac tggctgctgg agacagcaca aacccatcag gtggatgcga ttattgttgc
    29941 cggtgatgtt ttcgataccg gctcgccgcc cagttacgcc cgcacgttat acaaccgttt
    30001 tgttgtcaat ttacagcaaa ctggctgtca tctggtggta ctggcaggaa accatgactc
    30061 ggtcgccacg ctgaatgaat cgcgcgatat catggcgttc ctcaatacta ccgtggtcgc
    30121 cagcgccgga catgcgccgc aaatcttgcc tcgtcgcgac gggacgccag gcgcagtgct
    30181 gtgccccatt ccgtttttac gtccacgtga cattattacc agccaggcgg ggcttaacgg
    30241 tattgaaaaa cagcagcatt tactggcagc gattaccgat tattaccaac aacactatgc
    30301 cgatgcctgc aaactgcgcg gcgatcagcc tctgcccatc atcgccacgg gacatttaac
    30361 gaccgtgggg gccagtaaaa gtgacgccgt gcgtgacatt tatattggca cgctggacgc
    30421 gtttccggca caaaactttc caccagccga ctacatcgcg ctcgggcata ttcaccgcgc
    30481 acagattatt ggcggcatgg aacatgttcg ctattgcggc tcccccattc cactgagttt
    30541 tgatgaatgc ggtaagagta aatatgtcca tctggtgaca ttttcaaacg gcaaattaga
    30601 gagcgtggaa aacctgaacg taccggtaac gcaacccatg gcagtgctga aaggcgatct
    30661 ggcgtcgatt accgcacagc tggaacagtg gcgcgatgta tcgcaggagc cacctgtctg
    30721 gctggatatc gaaatcacta ctgatgagta tctgcatgat attcagcgca aaatccaggc
    30781 attaaccgaa tcattgcctg tcgaagtatt gctggtacgt cggagtcgtg aacagcgcga
    30841 gcgtgtgtta gccagccaac agcgtgaaac cctcagcgaa ctcagcgtcg aagaggtgtt
    30901 caatcgccgt ctggcactgg aagaactgga tgaatcgcag cagcaacgtc tgcagcatct
    30961 tttcaccacg acgttgcata ccctcgccgg agaacacgaa gcatgaaaat tctcagcctg
    31021 cgcctgaaaa acctgaactc attaaaaggc gaatggaaga ttgatttcac ccgcgagccg
    31081 ttcgccagca acgggctgtt tgctattacc ggcccaacag gtgcggggaa aaccaccctg
    31141 ctggacgcca tttgtctggc gctgtatcat gaaactccgc gtctctctaa cgtttcacaa
    31201 tcgcaaaatg atctcatgac ccgcgatacc gccgaatgtc tggcggaggt ggagtttgaa
    31261 gtgaaaggtg aagcgtaccg tgcattctgg agccagaatc gggcgcgtaa ccaacccgac
    31321 ggtaatttgc aggtgccacg cgtagagctg gcgcgctgcg ccgacggcaa aattctcgcc
    31381 gacaaagtga aagataagct ggaactgaca gcgacgttaa ccgggctgga ttacgggcgc
    31441 ttcacccgtt cgatgctgct ttcgcagggg caatttgctg ccttcctgaa tgccaaaccc
    31501 aaagaacgcg cggaattgct cgaggagtta accggcactg aaatctacgg gcaaatctcg
    31561 gcgatggttt ttgagcagca caaatcggcc cgcacagagc tggagaagct gcaagcgcag
    31621 gccagcggcg tcgcgttgct cacgccggaa caagtgcaat cgctgacagc gagtttgcag
    31681 gtacttactg acgaagaaaa acagttactt accgcgcagc agcaagaaca acaatcgcta
    31741 aactggttaa cgcgtcagga cgaattgcag caagaagcca gccgccgtca gcaggccttg
    31801 caacaggtgt tagccgaaga agaaaaagcg caacctcaac tggcggcgct tagtctggca
    31861 caaccggcac gaaatcttcg tccacactgg gaacgcatcg cagaacacag cgcggcgctg
    31921 gcgcatattc gccagcggat tgaagaagta aatactcgct tacagagcac aatggcgctt
    31981 cgcgcgagca ttcgccacca cgcggcgaag cagtcagcag aattacagca gcagcaacaa
    32041 agcctgaata cctggttaca ggaacacgac cgcttccgtc agtggaacaa cgaactggcg
    32101 ggttggcgtg cgcagttctc ccaacaaacc agcgatcgcg agcatctgcg gcaatggcag
    32161 caacagttaa cccatgctga gcaaaaactt aatgcgcttg cggcgatcac gttgacgtta
    32221 accgccgatg aagttgctac cgccctggcg caacatgctg agcaacgccc actgcgtcag
    32281 cgcctggtcg cgctgcatgg gcagattgtt ccccaacaaa aacgtctggc gcagttaatg
    32341 gtcactatcc agaatgtcac tctggaacag acgcaacgta atgtcgcact taacgaaatg
    32401 cgccagcgtt ataaagaaaa gacgcagcaa cttgccgatg tgaaaaccat ttgcgagcag
    32461 gaagcgcgca tcaaaacgct ggaagcccag cgtgcacagt tacaggcggg tcagccttgt
    32521 ccactttgtg gttccaccag ccatccggcg gtcgaggcgt atcaggcgct ggagcctggc
    32581 gttaatcagt ctcgattact ggcgctggaa aacgaagtta aaaagctcgg cgaagaaggt
    32641 gcggcgctgc gcgggcaact ggatgcatta acgaagcagc ttcagcgcga tgaaaacgaa
    32701 gcgcaaagcc tccgacaaga tgagcaagca cttactcaac aatggcaagc cgtcacggcc
    32761 agcctcaata tcaccttgca gccacaggac gatattcaac cgtggcttga tgcacaagat
    32821 gagcacgaac gccagctgcg gttactcagc caacgacatg aattacaagg gcagattgcc
    32881 gcgcataatc agcaaattat ccagtatcaa cagcaaattg aacaacgcca gcagcagctc
    32941 ttaacggcat tggcgggtta tgcactgaca ttgccacagg aagatgaaga agagagctgg
    33001 ttggcgacac gtcagcaaga agcgcagagc tggcagcaac gccagaacga attaaccgcg
    33061 ctgcaaaacc gtattcagca gctgacgccg attctggaaa cgttgccgca aagtgatgat
    33121 ctcccgcaca gcgaagaaac tgtggcgctg gataactggc ggcaggttca tgaacaatgt
    33181 ctcgcattac acagccagca gcagacatta cagcaacagg atgttctggc ggcgcaaagt
    33241 ctgcaaaaag cccaggcgca gtttgacacc gcgctacagg ccagcgtctt tgacgatcag
    33301 caggcgttcc ttgcggcgct aatggatgaa caaacactaa cgcagctgga acagctcaag
    33361 cagaatctgg aaaaccagcg ccgtcaggcg caaacgctgg tcactcagac agcagaaacg
    33421 ctggcacagc atcaacaaca ccgacctgac gggttggctc tcactgtgac ggtggagcag
    33481 attcagcaag agttagcgca aactcaccaa aagttgcgtg aaaacacgac gagtcaaggc
    33541 gagattcgcc agcagctgaa gcaggatgca gataaccgtc agcaacaaca aaccttaatg
    33601 cagcaaattg ctcaaatgac gcagcaggtt gaggactggg gatatctgaa ttcgctaata
    33661 ggttccaaag agggcgataa attccgcaag tttgcccagg ggctgacgct ggataattta
    33721 gtccatctcg ctaatcagca acttacccgg ctgcacgggc gctatctgtt acagcgcaaa
    33781 gccagcgaga cgcaggaagt cgaggttgtt gatacctggc aggcagatgc ggtacgcgat
    33841 acccgtaccc tttccggcgg cgaaagtttc ctcgttagtc tggcgctggc gctggcgctt
    33901 tcggatctgg tcagccataa aacacgtatt gactcgctgt tccttgatga aggttttggc
    33961 acgctggata gcgaaacgct ggataccgcc cttgatgcgc tggatgccct gaacgccagt
    34021 ggcaaaacca tcggtgtgat tagccacgta gaagcgatga aagagcgtat tccggtgcag
    34081 atcaaagtga aaaagatcaa cggcctaggc tacagcaaac tggaaagtac gtttgcagtg
    34141 aaataactat tcagcaggat aatgaataca gaggggcgaa ttatctcttg gccttgctgg
    34201 tcgttatcct gcaagctatc actttattgg ctacggtgat tggtagccgt tctggtagtt
    34261 gtgatggtgg tatgaaaaaa gtcattttat ctttggctct gggcacgttt ggtttgggga
    34321 tggccgaatt tggcattatg ggcgtgctca cggagctggc gcataacgta ggaatttcga
    34381 ttcctgctgc cgggcatatg atctcgtatt atgcgctggg agtggtggtc ggtgcgccaa
    34441 tcatcgcact cttttccagc cgctactcac tcaaacatat cttgttgttt ctggtggcgt
    34501 tgtgcgtcat tggcaacgcc atgttcacgc tctcttcgtc ttacctgatg cttgccattg
    34561 gtcgcctggt ttccggcttt ccgcatggcg cattttttgg cgtcggcgcg atcgtgttat
    34621 caaaaattat caaacccgga aaagtcaccg ccgccgtggc ggggatggtt tccgggatga
    34681 cagtcgccaa tttgctgggc attccgctgg gaacgtattt aagtcaggaa tttagctggc
    34741 gttacacctt tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt
    34801 gggtgccgga tattcgcgac gaggcgaaag gaaagctgcg cgaacaattt cactttttac
    34861 gcagcccggc cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg
    34921 cctggttcag ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga
    34981 tgacctttat tatgatgtta gttgggctag ggatggtgtt gggaaatatg ctaagtggca
    35041 ggatttcagg acgttattca ccactgcgca ttgcagcagt gactgacttt ataattgtac
    35101 tggcactgct gatgctcttt ttctgctgcg gcatgaaaac aacgtcgctt atttttgctt
    35161 ttatttgttg cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactacaaa
    35221 acgccaaagg cggagagtta ttaggtgccg caggtgggca aatagcgttt aacctcggta
    35281 gcgccgtcgg cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg
    35341 tggcgctgcc tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc
    35401 gctataagcg ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta
    35461 ctgtcacggt gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc
    35521 ggttctggca cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc
    35581 gccaattgcc tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct
    35641 ggaatcaccg tgcttcgcct tacgcaccgg cgtttcacat tcgccgccga agacaaattg
    35701 tttaatcaac tgcggaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag
    35761 gacaatcaca tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag
    35821 ctcgtagcga cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggctgataat
    35881 ttcactgcct ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atccagtgcc
    35941 cgaaataaag gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata
    36001 acgcagttcg tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc
    36061 attgccgccg atatgcgccc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat
    36121 aatcacggca aataccgtct gcgctcccgc tgccgcgcca tctaccgctt ctgaaaccgc
    36181 cagacagttg gcgtcatttg ccagccgcac ttcccgctgc aaccgcgcgc ttaagtcttt
    36241 atcgaatggc tgaccgttga gccaggttga attggcattc ttcaccacac cggtgtaggg
    36301 cgaaatcgaa cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc
    36361 catatcaacc aacgtggcga tcgtttcaat agtttgccgg taatcatcac gcggcgtggg
    36421 cagacgatgg cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt
    36481 gccgcctaaa tcgataccta tacgcacggt actctcctta tttttttcaa tatcaatagc
    36541 gtagagacgg acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc
    36601 taaatttaac gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta
    36661 ccgtcttagc cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat
    36721 ggcatttacc ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg
    36781 atcgcacagc gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa
    36841 agaagaaaaa atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa
    36901 actggaagcg gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga
    36961 agtgctgcac tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggat
    37021 cgacacggtt aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac
    37081 gctggcatta ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa
    37141 cccgattgaa ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca
    37201 gctgcttgat gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa
    37261 gaaacaagat ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac
    37321 taaactggcg ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct
    37381 caagcgtctg aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga
    37441 tttcgcccag cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat
    37501 tcaaaacctg attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta
    37561 atgccggatg cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aggcatataa
    37621 ttattcgcta cggcgggcaa taatttttag cgcagcaata ttatgcgttt tatgctgtaa
    37681 cttgctccat ggacgttgtg tcattgtttt tcctcaagcc agtgacgcgc ttcactgcga
    37741 attttccgca ttgccccggt tttcactaaa tcccgtaaca gcgaaagatc gggttgttcg
    37801 acaaataaga gtaatgatgg atcctctttt aaccacgtcg ttttcacatc ggcagcgagc
    37861 tgtggattat tcatagctag cgaccgatgc cgagactcgg tcaatgtcgt taacaacgac
    37921 gccggggtat gtgcgttgcg taatgccgtt tggcgcaaat gatcattgcg actttgcagc
    37981 aatgcacgcc accattcatc atctgattta tcttttatcg tagccagttc cgcaacaata
    38041 tctggagatc ttcactgata tttatatcaa ccttgatgaa ttatgaatat accggaaatt
    38101 gaaaaaatcg cagcaaagga aatattatta tgggttaaag agaggatatt tcagaaaaat
    38161 gccctcccgt tcaaggggaa ggcgaggaat tacagatagc ggcacagata agaggtgggt
    38221 tcggcaactt gcagatgaaa ctcactgtga ccgggaacat taaacaccga accggcttca
    38281 tacacctgcc agtcggtcgc gtcaggcagt aacacattca gcgcgccact gattaccgtc
    38341 atctcttccg gctcagcggt gctgaaggtg tattcgcctt caaccataac acccacgctg
    38401 gcgcgaccag tgctgctgct ggaaaagccg attgatttca ctttgccgga aaagtactca
    38461 ttactttgaa gcataaactg gccctgtctg gtagttgaac atctacttca ctataggggc
    38521 cagaggcgct ggctgtcacg caaaattaca ccagtaattc cgcagccagc cgtgcaatca
    38581 atacgttaga cagcaagaca ggaacatcga gctgtttttg cagtaaatcg cgatgacgtt
    38641 ggtgaaatcc taaacaatcc agcatgatga catcagcccc ttttgccagt aattctttcc
    38701 cggcatcaat gattttttgt tctgaatcat gaatggggtt gcccaatgaa aataccggcg
    38761 atttctgcaa aatttgccat ttttgcgcct gaacggtcag catctcctca accggaacga
    38821 taacccccac ctgatgatct tcaacaatag aggaaaccag tggaggcaat attcgcgacg
    38881 gctcaagaaa gatcgtatta cgcgcagtca tactactaat gtttgctgta ctcattaata
    38941 aaatgacgtc ataaccctga ttatcgagca cttcaaccac accttgcagg tctcgctcca
    39001 ctttacgacg cgaaacatgg gccagctggt tgtcatttaa taatgtgaga atggtgtctt
    39061 cgcctgcttc tggcgcgtac tccgccatca cttcttcacg acttaacttc cccagcaagc
    39121 tatgatggga aatattatct tcgtcaatgt attccgtcag gagcggcaaa acctcctgca
    39181 taggtacaat gccgatggtc aggatcgcca acgacgcact cattgttcat ccaccttact
    39241 tttctttcgt cgttaattac cgggcaagtg tgaagcacca ctgctgacac ttacttgtgc
    39301 tacaaatgac aaaaagcgta gcagcagacg tgcacggcat aatgtaaaga ttccaaatga
    39361 ttccagtaat ggattgttat tgtttaatat tctaattatt agaaaaacat gaattatgaa
    39421 aaatgtgacg cagatccctt gtagtggcac tcagaatccc ttccgagcag tctgatgcgg
    39481 tcactctttg tcagggtcct catagcgttt tttcgcatcc atcgcttcct gagccgtcgg
    39541 atgttcactg atcaacgagt ctggtttagg atgatcggct ctgagttggt accaggttac
    39601 cgtctgtcct ggctttcctt tttcaatcgt cactatatat gcttcacgag gataaggcgg
    39661 tttcgttggc atcgttcttc cttatttcac gggatgaacg ttaagtatag gcgctctaaa
    39721 atcaacaatt gatcgtctgt gccagggcgc tacgaatttc agaaatcacc tggctgggtt
    39781 cgtttgttgc gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca
    39841 gcacttcctg aacttcttcg ctcagcggtt ttcccgttaa ggttggccgt aaatcttctt
    39901 ccggtgcagc ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa taaaccacga
    39961 tcccgttatt ttgcatgaag tgacgattaa attccgtcag aataatgccg ccgcctgtag
    40021 cgataacggt ggatggcgca gttaccgctt ccagcgccgc cgtttctctg gcgcgaaatc
    40081 ccgcccactc ttccctttcg acgatctccg cgaccgtcat attgagctgt gattgcaacc
    40141 actgatcggt atcgacaaac cgacggttaa gcgaatcggc aagggccatt ccgaccgttg
    40201 ttttaccaca gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg ggttttcccc
    40261 aataggtcgc aatgcggcga aagccggtgt catgagaata gcgatcatgc catcaaacta
    40321 gtacaatttc gattgtaaag aaaaaattcc acttaaagtg aaaatctcaa tacatccctt
    40381 actataccaa taaatattca agaatgaagt gtaaataata aattacattt agccacgact
    40441 acgttgcact tccagccacc acttctccag ctccgcggca aaggcctggc ggtcacgttg
    40501 tgaaaggcta tctggtccgc cggtctggat cccactggca cgtaaggtat ccataaaatc
    40561 gcgcatcgtc aggcgctcac gaatggtcgc tggcgtgtaa cgttcgccgc gcggattaag
    40621 cgccgctgcg cctttctcga tggcttcagc agccaaaggt atatctgcgg tgatcaccaa
    40681 atcgcccgct tcacactgcc ggacaatttc gttatcggca acgtcgaaac ctgccgcgac
    40741 gcgcagcgta cgaataaatc gcgatggcgg cacgcgtaaa ctctggtttg ctaccagtac
    40801 cagcggcatc tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta cattgggaca
    40861 cgcgtcggca tccacccaaa ttgtcataaa gtcatccttt gttgggtaat cctctattgt
    40921 gtcgcgcttt tgccttccgg catagttctg tttatgcttc tgccagcgat tatcaaaaca
    40981 atgaatttca cggcaggagt gaggcaatgg aaaagaaaat cggttttatt ggctgcggca
    41041 atatgggaaa agccattctc ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa
    41101 tctgggtata caccccctcc ccggataaag tcgccgccct gcatgaccag ttcggcatca
    41161 acgccgcaga atcggcgcaa gaagtggcgc aaatcgccga catcattttt gctgccgtta
    41221 aacctggcat catgattaaa gtgcttagcg aaatcacctc cagcctgaat aaagactctc
    41281 tggtcgtttc tattgctgca ggtgtcacgc tcgaccagct tgcccgcgcg ctgggccatg
    41341 accggaaaat tatccgcgcc atgccgaaca ctcccgcgct ggttaatgcc gggatgacct
    41401 ccgtaacgcc aaacgcgctg gtaaccccag aagataccgc tgatgtgctg aatattttcc
    41461 gctgctttgg cgaagcggaa gtaattgctg agccgatgat ccacccggtg gtcggtgtga
    41521 gcggttcttc gccagcctac gtatttatgt ttatcgaagc gatggccgac gccgccgtgc
    41581 tgggcgggat gccacgcgcc caggcgtata aatttgccgc tcaggcggta atgggttccg
    41641 caaaaatggt gctggaaacg ggagaacatc cgggggcact gaaagatatg gtctgctcac
    41701 cgggaggcac caccattgaa gcggtacgcg tactggaaga gaaaggcttc cgtgccgcag
    41761 tgatcgaagc gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa tcctgatgac
    41821 tttcgccgga cgtcaggccg ccacttcggt gcggttacgt ccggctttct ttgctttgta
    41881 aagcgccaaa tctgccgatt tcaaccactc acgatagtga ctcatttgtg ggttcagcgg
    41941 cgcaaccccc acactaatcc gtaaagttac ctgtggcgta ttcggcaagc gtaacgtgtt
    42001 tagcccttca cgcacccgta acatggcggt aatggcgctc tcagctggcg taccggacat
    42061 gattactgca aactcatcgc cgccaaaccg accaatcaca tcgctaccgc gcagggtaat
    42121 ttgtaactgt cgggtaagcg ccacaatcgc ttcatcgccc acatcatgac cccaggtatc
    42181 gttgatgctc ttgaaatggt cgatatcgat aatcagtaac gttgcatcgc gattatgccg
    42241 ccgacagtta tcaaattcat tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc
    42301 cgtcatgccg tcgcgggtac tcatgacctg caacctgcgt ttatgttccg ccagtttggt
    42361 tgccgtctgg tagctgaccc agccaaacag cagaggataa acgacaataa tgggaaggga
    42421 gagccaccat tccagcggcg cactattgaa cgacacggta atgcccgtaa gctcgagggt
    42481 gacaaggcag gaaaccacca tcaacaccag acccgcgaca aacagacggg ggccgcctgc
    42541 ccccatcaaa ttcagacaca taatcatcaa catagcggtg gaaggcagca cgtttacgcc
    42601 cattacgcct acccacattc ccgctaatac tgcatcggtt tttaagttgt aaatttcccg
    42661 gctaagcgga tcgacggccc tgctcgctat ctgccaggct aaatgcggcc agacgaacgc
    42721 ccagccgacc aacaccagcc accaccagcc cggcggcggg tgcgaaacca gcgttgaagc
    42781 aatcggtaag aacatgccag ccaggccaac cgcacggggt agtctgacgc gacgggcgaa
    42841 gcgcagcccg gaccgctgat gttcgttttg aggagttaaa ggaggttcct ccccatgcgc
    42901 cgccgctttt ttgaaaaagt tttcatcatt cattattttt gggaacattc agaaacaatt
    42961 ttcccaaatt atagagacgg ggctaaaggc agaagtatga aatttcgggt gagccacttt
    43021 cgtagctcac ccgttagcac tatcaggtat caggcggctt tcttgaggca ggcactcata
    43081 aacttattac gatcatcacc tttcagagat tgttgcgttg cttgattatt acattcgcgc
    43141 atcttttgct gctgtggcgt caaacttttt tcaccaggcg cagacttgct gttcttcagg
    43201 caatcactca tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt cgcctgctga
    43261 ttacaggatg tcatacgctg ttgttgtggg gttaaggttc tctcggcagc gccgacggtg
    43321 gttaaaaaaa ccagaccaaa aagcaaggta accagtaatg ttattttcat agcaccatcc
    43381 ctcttcatgt tttaaccatg agcgtatgcg cccgtgatct gccattaagt ctggttgcta
    43441 acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt atttcagccc
    43501 cagggcggct ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa caacattggc
    43561 ggcatgcggg ccatacgccg caatacgcaa ctgactgccg gtatgttctt gtgaatcctc
    43621 ttcggagttc ccgtagctca tcaccatcac tgcgccatct ttggtattta gcgcctgggt
    43681 gaggcccgga gctttggtat ccggcgcgac aatctggctg gcgtgggcgt gatcagcggt
    43741 gactatgacc agcgtgttgc catccttttt agcgaattcc agcgcccgtt gtacggcttc
    43801 atcgagatcg accgtctcgc caatttgccc acaaggattc gcagcgtgat cctgtttatc
    43861 gattgacgca ccttcaactt gcaggaaaaa tcctttctca tttttactca acaattcaat
    43921 ggctttgtcg gtcatctgcg ccagggttgg tacactgtca ttacgttgcg gattaggcgt
    43981 acaggtgact gcgggcttat cgatattgcc gtggtacgtt gctttcggtc ctagccagcg
    44041 cactggcata ttgccgtcag caaacagtcc aagcagaggt ttttgctgat tcgcttccgt
    44101 caccgaattc agtgaggcag catcgctgac caactgataa ccacgcgcct gtgcctgttc
    44161 acgcagcgtt tttccctgcc attcaccggc ggttgccgtt tcagcaaagg tttttgcgcc
    44221 accgccaagc gtaacgtcgg cacgagcgtt aagcagctgt tcggtaatcg atccttttcc
    44281 gcctttttcc agagcgttac ccggacattt ttcactggtc gcgctcggac cgtagcattt
    44341 gcgcgaggtc acatgcgcca ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga
    44401 aacgttaccg gtcgccagac ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt
    44461 ttcgtgaata tcgacgccca gcgcgccgtt ataggttttg acaccggttg accaggcggt
    44521 tgctgatgca gccgaatcgg tgacgtagtc cggtttgccg gtttttttat tcagcgcata
    44581 gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc
    44641 ttcggcataa ttacgtgcgg ctgtaatttc cgaatccccc atcccatcgc caatcagcaa
    44701 aataatattt tttgcaggtt tatcgctaag agaatcacgc agagcggcag tctgatcacc
    44761 cgttaaacgg cgagcaccgc cggatgcagt aatatcgccc tgagcagccc ggttttccag
    44821 aacaggcatt tctggtgtcc gggcttttgt cacaggggta aacagtaacg ataagagtgc
    44881 cagtgcaata gtgctttgtt tcactttatt ttctccatgt acaaatacat taaaaaataa
    44941 aaacaaagcg actataagtc tcggccgtga caactttatg acagctgttg aaaagattaa
    45001 ctttttactg acgaggatgc ttcaataact tctttacgta atcgcgcagc agctccgtat
    45061 cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa
    45121 tcaagcgctg ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg ataatctcca
    45181 aagcttctac ctgcgcacac agttctttcg actcttcttc tttttgggca agcttaaata
    45241 acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat ggagaagtta
    45301 acattatgtt t
//
LOCUS       DAZFRJ010000014        44627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000014 DAZFRJ010000000
VERSION     DAZFRJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.014"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(172..1536)
                     /gene="mnmE"
                     /locus_tag="ACHKUN_000716"
     CDS             complement(172..1536)
                     /gene="mnmE"
                     /locus_tag="ACHKUN_000716"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO6659028.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1642..3288)
                     /gene="yidC"
                     /locus_tag="ACHKUN_000717"
     CDS             complement(1642..3288)
                     /gene="yidC"
                     /locus_tag="ACHKUN_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO6659029.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3291..3548)
                     /gene="yidD"
                     /locus_tag="ACHKUN_000718"
     CDS             complement(3291..3548)
                     /gene="yidD"
                     /locus_tag="ACHKUN_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO6659030.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3512..3871)
                     /gene="rnpA"
                     /locus_tag="ACHKUN_000719"
     CDS             complement(3512..3871)
                     /gene="rnpA"
                     /locus_tag="ACHKUN_000719"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO6659031.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3888..4028)
                     /gene="rpmH"
                     /locus_tag="ACHKUN_000720"
     CDS             complement(3888..4028)
                     /gene="rpmH"
                     /locus_tag="ACHKUN_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO6659032.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4258..4338)
                     /locus_tag="ACHKUN_000721"
     CDS             complement(4258..4338)
                     /locus_tag="ACHKUN_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659033.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4635..6038
                     /gene="dnaA"
                     /locus_tag="ACHKUN_000722"
     CDS             4635..6038
                     /gene="dnaA"
                     /locus_tag="ACHKUN_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO6659034.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6043..7143
                     /gene="dnaN"
                     /locus_tag="ACHKUN_000723"
     CDS             6043..7143
                     /gene="dnaN"
                     /locus_tag="ACHKUN_000723"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO6659035.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7143..8216
                     /gene="recF"
                     /locus_tag="ACHKUN_000724"
     CDS             7143..8216
                     /gene="recF"
                     /locus_tag="ACHKUN_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO6659036.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8245..10659
                     /gene="gyrB"
                     /locus_tag="ACHKUN_000725"
     CDS             8245..10659
                     /gene="gyrB"
                     /locus_tag="ACHKUN_000725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO6659037.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10899..11297
                     /gene="yidB"
                     /locus_tag="ACHKUN_000726"
     CDS             10899..11297
                     /gene="yidB"
                     /locus_tag="ACHKUN_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO6659038.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11412..12224
                     /gene="yidA"
                     /locus_tag="ACHKUN_000727"
     CDS             11412..12224
                     /gene="yidA"
                     /locus_tag="ACHKUN_000727"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO6659039.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12270..12926)
                     /gene="yidX"
                     /locus_tag="ACHKUN_000728"
     CDS             complement(12270..12926)
                     /gene="yidX"
                     /locus_tag="ACHKUN_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO6659040.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13204..13893
                     /gene="dgoR"
                     /locus_tag="ACHKUN_000729"
     CDS             13204..13893
                     /gene="dgoR"
                     /locus_tag="ACHKUN_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO6659041.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13890..14768
                     /gene="dgoK"
                     /locus_tag="ACHKUN_000730"
     CDS             13890..14768
                     /gene="dgoK"
                     /locus_tag="ACHKUN_000730"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO6659042.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14752..15369
                     /gene="dgoA"
                     /locus_tag="ACHKUN_000731"
     CDS             14752..15369
                     /gene="dgoA"
                     /locus_tag="ACHKUN_000731"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO6659043.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15366..16514
                     /gene="dgoD"
                     /locus_tag="ACHKUN_000732"
     CDS             15366..16514
                     /gene="dgoD"
                     /locus_tag="ACHKUN_000732"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO6659044.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16589..17926
                     /gene="dgoT"
                     /locus_tag="ACHKUN_000733"
     CDS             16589..17926
                     /gene="dgoT"
                     /locus_tag="ACHKUN_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6659045.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17923..18987)
                     /gene="cbrA"
                     /locus_tag="ACHKUN_000734"
     CDS             complement(17923..18987)
                     /gene="cbrA"
                     /locus_tag="ACHKUN_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO6659046.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            19088..20302
                     /gene="yidR"
                     /locus_tag="ACHKUN_000735"
     CDS             19088..20302
                     /gene="yidR"
                     /locus_tag="ACHKUN_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO6659047.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20304..20636)
                     /gene="yidQ"
                     /locus_tag="ACHKUN_000736"
     CDS             complement(20304..20636)
                     /gene="yidQ"
                     /locus_tag="ACHKUN_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6659048.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20876..20948
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20942..21355
                     /gene="ibpA"
                     /locus_tag="ACHKUN_000737"
     CDS             20942..21355
                     /gene="ibpA"
                     /locus_tag="ACHKUN_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO6659049.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21467..21895
                     /gene="ibpB"
                     /locus_tag="ACHKUN_000738"
     CDS             21467..21895
                     /gene="ibpB"
                     /locus_tag="ACHKUN_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO6659050.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22092..23753
                     /gene="yidE"
                     /locus_tag="ACHKUN_000739"
     CDS             22092..23753
                     /gene="yidE"
                     /locus_tag="ACHKUN_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO6659051.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDMPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23750..24466)
                     /gene="yidP"
                     /locus_tag="ACHKUN_000740"
     CDS             complement(23750..24466)
                     /gene="yidP"
                     /locus_tag="ACHKUN_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6659052.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24762..26378
                     /locus_tag="ACHKUN_000741"
     CDS             24762..26378
                     /locus_tag="ACHKUN_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6659053.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26378..27192
                     /locus_tag="ACHKUN_000742"
                     /pseudo
     CDS             26378..27192
                     /locus_tag="ACHKUN_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27189..28082)
                     /gene="yidL"
                     /locus_tag="ACHKUN_000743"
     CDS             complement(27189..28082)
                     /gene="yidL"
                     /locus_tag="ACHKUN_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6659054.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28249..29964
                     /gene="yidK"
                     /locus_tag="ACHKUN_000744"
     CDS             28249..29964
                     /gene="yidK"
                     /locus_tag="ACHKUN_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO6659055.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29961..31454
                     /gene="yidJ"
                     /locus_tag="ACHKUN_000745"
     CDS             29961..31454
                     /gene="yidJ"
                     /locus_tag="ACHKUN_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6659056.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31501..31950)
                     /gene="yidI"
                     /locus_tag="ACHKUN_000746"
     CDS             complement(31501..31950)
                     /gene="yidI"
                     /locus_tag="ACHKUN_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO6659057.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            32060..32407
                     /gene="yidH"
                     /locus_tag="ACHKUN_000747"
     CDS             32060..32407
                     /gene="yidH"
                     /locus_tag="ACHKUN_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO6659058.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32397..32759
                     /gene="yidG"
                     /locus_tag="ACHKUN_000748"
     CDS             32397..32759
                     /gene="yidG"
                     /locus_tag="ACHKUN_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO6659059.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32756..33253
                     /gene="yidF"
                     /locus_tag="ACHKUN_000749"
     CDS             32756..33253
                     /gene="yidF"
                     /locus_tag="ACHKUN_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO6659060.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33261..34445)
                     /gene="emrD"
                     /locus_tag="ACHKUN_000750"
     CDS             complement(33261..34445)
                     /gene="emrD"
                     /locus_tag="ACHKUN_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO6659061.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34527..34601
                     /gene="ysdE"
                     /locus_tag="ACHKUN_000751"
     CDS             34527..34601
                     /gene="ysdE"
                     /locus_tag="ACHKUN_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO6659062.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34864..34953)
                     /gene="tisB"
                     /locus_tag="ACHKUN_000752"
     CDS             complement(34864..34953)
                     /gene="tisB"
                     /locus_tag="ACHKUN_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO6659063.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35518..35616
                     /gene="ivbL"
                     /locus_tag="ACHKUN_000753"
     CDS             35518..35616
                     /gene="ivbL"
                     /locus_tag="ACHKUN_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO6659064.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35722..37410
                     /gene="ilvB"
                     /locus_tag="ACHKUN_000754"
     CDS             35722..37410
                     /gene="ilvB"
                     /locus_tag="ACHKUN_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO6659065.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37414..37704
                     /gene="ilvN"
                     /locus_tag="ACHKUN_000755"
     CDS             37414..37704
                     /gene="ilvN"
                     /locus_tag="ACHKUN_000755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6659066.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37779..38369
                     /gene="uhpA"
                     /locus_tag="ACHKUN_000756"
     CDS             37779..38369
                     /gene="uhpA"
                     /locus_tag="ACHKUN_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO6659067.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38369..39871
                     /gene="uhpB"
                     /locus_tag="ACHKUN_000757"
     CDS             38369..39871
                     /gene="uhpB"
                     /locus_tag="ACHKUN_000757"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO6659068.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39881..41200
                     /gene="uhpC"
                     /locus_tag="ACHKUN_000758"
     CDS             39881..41200
                     /gene="uhpC"
                     /locus_tag="ACHKUN_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6659069.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            41456..42847
                     /gene="uhpT"
                     /locus_tag="ACHKUN_000759"
     CDS             41456..42847
                     /gene="uhpT"
                     /locus_tag="ACHKUN_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO6659070.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42892..44625)
                     /gene="adeD"
                     /locus_tag="ACHKUN_000760"
     CDS             complement(42892..44625)
                     /gene="adeD"
                     /locus_tag="ACHKUN_000760"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO6659071.1"
                     /translation="MSRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIK
                     GRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLT
                     TVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQM
                     LAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITA
                     GIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRN
                     PWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVL
                     LSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFT
                     PGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFG
                     LNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIA
                     GLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGE
                     KFAFTTLEVTE"
BASE COUNT        10287 a        11464 c        12125 g        10751 t
ORIGIN      
        1 taaatgtgaa tcgaatcaca atcgttcggg gagcaatatt aaatgctttt ttttaagatt
       61 ataaattacg taagaatttt aatgtttaaa aaaagggttg aggaataaca ggagtgctaa
      121 caatgaagcg aagccgcatc tgacagtcag aatacggctt cgtaagcgcg gttacttacc
      181 aatacagaag ctggagaaaa tccgccccag caggtcgtct gaagtaaatt ccccggtgat
      241 ttcgcttaag ttctgctgtg ccaggcgcaa ctcttccgcc agcagttcac ccgcccaggc
      301 acctaacagt tgcgctttac cttgctgcaa atgctccgct gcctgttcca gcgcctgtaa
      361 gtggcgacgg cgcgccagga aaccgccttc catgttggtg tcaaagccca tgctctgttt
      421 gagatggtta cgcagcacgt ccacgccttc accagtcctt gccgagagac gaattaacgc
      481 gtgaccgttc acttcactca ttcccagcgt ttcgccggtg atatcggctt tattgcgcac
      541 cacggtgatc ggcagtttcg ctggcagacg ggcgataaat tccggccaga tctctgccgg
      601 atccacggcg tctgttgtgg tgccatcgac cataaacagc acccggtcgg cctgttcaat
      661 ttcctgccac gcgcgctcga tcccaatacg ttccacttcg tcactggctt cacgtagccc
      721 ggcggtgtcg atgatatgca gcggcattcc gtcaatgtgg atatgctcac gcaacacgtc
      781 acgcgtagtt ccggcgatat cggttacgat tgctgcttca cggccagcca gcgcgtttaa
      841 caggctcgat ttaccggcgt taggacgtcc ggcaatcacc actttcatcc cttcgcgcaa
      901 caaactaccc tgacgtgctt cagcacgcac tgcatcaaga tcggcaataa cgtcattgag
      961 ctgggcttca attttgccgt cggagaggaa atcgatctct tcatcaggga aatcaattgc
     1021 cgcttcgacg taaatgcgca agtgggtgag ggcttctacc agatgattaa cccgtgcgga
     1081 gaatgcgcct tgcagcgagt taagtgccga acgggccgcc tgttccgagc tggcgtcgat
     1141 aagatcggca atcgcctcag cctgggctaa gtcgagttta tcgtaaagaa acgcgcgttc
     1201 ggaaaactca ccagggcgag caatccgcag gccgggaatg gtcagaatgc gttttaacag
     1261 caggtcgagg atcaccggac cgccatgacc ctgcagttca agcacatctt cgccggtgaa
     1321 cgagttcggg ccagggaacc atagcgcaat cccctgatcg agcacgctgc cgtcggcgtc
     1381 tttaaatggc aaataatcgg cgtagcgcgg cttaggtagt ttacccagca cggtttcggc
     1441 aacttcacgg gctttgaggc cggagatgcg caggatgcca acaccgccac gtcccggagg
     1501 cgtggcctgg gctacgatag tgtcattatc gctcatgatg ttcctgttgc tttgtgtggc
     1561 ggatgcgcgg tgcttatccg ccctacgaaa agaaaaaagg cggtcaactg accgccctta
     1621 ttttagcgaa aactcaccga atcaggattt tttcttctcg cggctatgca ggccacgttt
     1681 ttccagacca cggtaaatca gctgctgctg aataatggtt accaggttgc tgacgatata
     1741 gtacagcacc agacctgacg ggaaccacag gaagaacacg gtgaagatga ccggcataaa
     1801 ggtcatgatc ttctgctgca tcgggtcggt cactgtggtc ggcgacatct tctgaatgaa
     1861 gaacatcgtt acgcccatca ggatcggcag gatgtagtac gggtcctgtg ccgacaggtc
     1921 gtggatccac agtgcaaacg gtgcctgacg cagttcaacg gaacccatca gcatgtagta
     1981 caacgccagg aagattggca tctggatcag cagcgggaag cagccgccca gcgggttaac
     2041 cttctcagct ttgtacagcg ccatcatttc ctggctgata cgctgtttgt catcgcccag
     2101 acgctcacgc attgcctgaa tcttcggctg cagcatacgc atcttcgcca tggaggtgta
     2161 ctgcgctttg gtcagcgggt acatgatgcc acgaacgata aaggtgatga tgataatgga
     2221 gaagccccag ttacccacaa agctatggat ccatttcagc agtttgaaca gcggctgaga
     2281 gatgaaccac aaccaaccgt aatcaacggt cagatccagg tgcggagcaa cagctgccat
     2341 tttgtcctgg atttccgggc caacccacag ggtgctgttc atcgcgccag tctgaccagg
     2401 ctgaaccagt accggctgag atttatagcc gatagcggcg atgccgttac ccagattagc
     2461 ggtatagaag ttgttggtac cgtcgttatg cgggatccac gccgtcgcga aatactgttg
     2521 cagcatcgcc acccaaccac ctttcgaaga gatgttcagg ttttcgttat cggcaatggt
     2581 atcgaacttg tatttctcat acttctcgtc aggcgtggag tacgccgcgc cacggaaggt
     2641 gtgcagtgcg aagttgctgc ttccggtatc gagatgcggt ggcagagtga tggattgctt
     2701 caactgacca aaggtggaga tttccagcgg tttctcgcca gcgttctgca cgttgtagtt
     2761 gacgttgaca gcgtaatcac cacgtttcag gacaaacgtt ttggtaaacg tgttgcctgc
     2821 cgcgtcggta tacgtcatcg gcacctgcag ttcgttttga ccttcagcca gcacataagc
     2881 gtctttttca acgttataca gcggacgcgg gccgttagcc gggttatccg ggccatcacg
     2941 accggtcaga ccgctctgtg cctgataaat aaactgcggt gaagtttcca gcagctggaa
     3001 cggctgggta gagttcagct ctttcgggta agcaggcagc agagcttgct caacatcacc
     3061 accacgggtg ttgatggtca gatcaagcac gtcggtctta accgagatca gtttcccctg
     3121 gccactggcc ggtacgccct ggtcggcggc gctacccgct gcggtggtcg ttgtctgcgt
     3181 ggtctgttgg gcctgaggtt gcgggttttt atcctgctcc caggcttgcc agatcatgaa
     3241 agacacgaac agcaaagcga tgactaaaag attgcgttgc gaatccatcg ttagtgttct
     3301 ctggtatcaa atggtccggg cgggacggga tcgtcaccac cagggtgtaa agggtggcat
     3361 tttaatacgc gtttcaccgt caaccaactg ccttttatca ctccaaacct gcgcaatgcc
     3421 tcaattccgt agcttgaaca ggttggagtg aaacgacaat gcggcccgag tagcggacta
     3481 atcaggcgtt gatagacccg aatgagggct atcaggaccc gcgagccagg cgacagtggc
     3541 ggcgccataa tttttccaac gcttccgaga gagcacggtt atcgaggtcg gcaacccctt
     3601 ttttcgccac caccacgaaa tccatagccg ggagttcatg ttggcgcaga cggaagcttt
     3661 cacgcgtcag acgtttaatc cgattgcgtt catgggcgcg tcgaacgttt ttcttggcga
     3721 ctgtaagacc gatacgggga tgccccagcg aattcaggcg gccgagaatg gtaatttgcg
     3781 gcgtgccagc ccgttgtggc tgctggaaga cgaatgtgaa ttgactggga gttaacaagc
     3841 gtaactccct gggaaatgcg agcttaacca ctcaggggtt agctttatta cttagaaacg
     3901 gtcagacgag cgcggccttt agcacgacga cgtgccagaa cctgacgacc atttttagta
     3961 gccatacgag cacggaagcc gtgagaacgg ttgcgcttca gtacagacgg ttgaaaagtg
     4021 cgtttcatgg cgatttctac ctaaacttga ataaattcaa tggctttatt ggatatccgc
     4081 cgaaaaatga aacgatggac accgaagcca tgggtgatta aagaggccgg attgtaataa
     4141 ttgtacactc cggagtcaat tctctttcct tatttaccgc gcttttccgc accttttcgc
     4201 agggaaaatg tacgacctca caccagtgaa aaccagcatg gcgcgccggg tggaggatta
     4261 tacgggctga tgggtaaagc gcaaggatcg tcctggatct ttattagatc gattaagcca
     4321 atttttgtct atggtcatta aattttccaa tatgcggcgt aaatcgtgcc cgcctcgcgg
     4381 caggatcgtt tacacttagc gaggtctgga aagtcctgtg gataaatcgg gaaaatctgt
     4441 gagaaacaga agatctcttg cgcagtttag gctatgatcc gcggtcccga tcgttttgca
     4501 ggatcttgat cgggcatata accgcagaca gcggttcgtg cgtcaccctc aagcagggtc
     4561 ttttcgacgt acgtcaacaa tcatgaatgt ttcagcctta gtcattatcg acttttgttc
     4621 gagtggagtc cgccgtgtca ctttcgcttt ggcagcagtg tcttgcccga ttgcaggatg
     4681 agttaccagc cacagaattc agtatgtgga tacgcccatt gcaggcggaa ctgagcgata
     4741 acacgctggc cctgtacgcg ccaaaccgtt ttgtcctcga ttgggtacgg gacaagtacc
     4801 ttaataatat caatggactg ctaaccagtt tctgcggagc ggatgcccca cagctgcgtt
     4861 ttgaagtcgg caccaaaccg gtgacgcaaa cgccacaagc ggcagtgacg agcaacgtcg
     4921 cggcccctgc acaggtggcg caaacgcagc cgcaacgtgc tgcgccttct acgcgctcag
     4981 gttgggataa catcccggcc ccggcagaac cgacctatcg ttctaacgta aacgtcaaac
     5041 acacgtttga taacttcgtt gaaggtaaat ctaaccaact ggcgcgcgcg gcggctcgcc
     5101 aggtggcgga taaccctggc ggtgcctata acccgttgtt cctttatggc ggcacgggtc
     5161 tgggtaaaac tcacctgctg catgcggtgg gtaacggcat tatggcgcgc aagccgaatg
     5221 ccaaagtggt ttatatgcac tccgagcgct ttgttcagga catggttaaa gccctgcaaa
     5281 acaacgcgat cgaagagttt aaacgctact accgttccgt agatgcactg ctgatcgacg
     5341 atattcagtt ttttgctaat aaagaacgat ctcaggaaga gtttttccat accttcaacg
     5401 ccctgctgga aggtaatcaa cagatcattc tcacctcgga tcgctatccg aaagagatca
     5461 acggcgttga ggatcgtttg aaatcccgct tcggttgggg actgactgtg gcaatcgaac
     5521 cgccagagct ggaaacccgt gtggcgatcc tgatgaaaaa ggccgacgaa aacgacattc
     5581 gtttgccggg cgaagtggcg ttctttatcg ccaagcgtct acgatctaac gtacgtgagc
     5641 tggaaggggc gctgaaccgc gtcattgcca atgccaactt taccggacgg gcgatcacca
     5701 tcgacttcgt gcgtgaggcg ctgcgcgact tgctggcatt gcaggaaaaa ctggtcacca
     5761 tcgacaatat tcagaagacg gtggcggagt actacaagat caaagtcgcg gatctccttt
     5821 ccaagcgtcg atcccgctcg gtggcgcgtc cgcgccagat ggcgatggcg ctggcgaaag
     5881 agctgactaa ccacagtctg ccggagattg gcgatgcgtt tggtggtcgt gaccacacga
     5941 cggtgcttca tgcctgccgt aagatcgagc agttgcgtga agagagccac gatatcaaag
     6001 aagatttttc aaatttaatc agaacattgt catcgtaaac ctatgaaatt taccgtagaa
     6061 cgtgagcatt tattaaaacc gctacaacag gtgagcggtc cgttaggtgg tcgtcctacg
     6121 ctaccgattc tcggtaatct gctgttacag gttgctgacg gtacgttgtc gctgaccggt
     6181 actgatctcg agatggaaat ggtggcacgt gttgcgctgg ttcagccaca cgagccagga
     6241 gcgacgaccg ttccggcgcg caaattcttt gatatctgcc gtggtctgcc tgaaggcgcg
     6301 gaaattgccg tgcagctgga aggtgaacgg atgctggtac gctccgggcg tagccgtttt
     6361 tcgctgtcta ccctgccagc ggcggatttc ccgaacctcg atgactggca gagtgaagtc
     6421 gaatttaccc tgccgcaggc aacgatgaag cgtctgattg aagcgaccca gttttcgatg
     6481 gcgcatcagg acgttcgcta ttacttaaat ggtatgctgt ttgaaaccga aggtgaagaa
     6541 ctgcgcaccg tggcaaccga cggccaccgt ctggcggtct gttcaatgcc aattggtcaa
     6601 tctttgccaa gccattcggt gatcgtaccg cgtaaaggcg tgattgaact gatgcgtatg
     6661 ctcgacggcg gcgacaatcc gctgcgcgtg cagattggca gcaacaatat tcgcgcccac
     6721 gttggcgact ttatcttcac ctccaaactg gtggatggtc gcttcccgga ttaccgccgc
     6781 gttctgccga agaatccgga caaacatctg gaagctggct gcgatctgct caagcaggcg
     6841 tttgcccgtg cggcaattct ctctaacgag aaattccgcg gcgtgcgcct gtatgtcagc
     6901 gaaaaccagc tgaaaatcac cgccaacaac ccggaacagg aagaagcgga agagatcctc
     6961 gacgttacct atagcggtgc ggagatggaa atcggcttca acgtcagcta tgtgctggat
     7021 gttctgaacg cgctgaaatg cgaaaacgtc cgcatgatgc tgaccgattc ggtttccagc
     7081 gtgcagattg aagatgccgc atcacagtcg gctgcctatg ttgtcatgcc aatgagactg
     7141 taatgtccct cacccgcttg ttgatccgcg atttccgcaa cattgaaacc gcggatctcg
     7201 ctttatctcc cggctttaac tttctggtag gtgccaacgg cagtggcaaa accagcgtgc
     7261 tggaagccat ctatacgctc ggccatggtc gggcgtttcg cagtttgcag attggtcgcg
     7321 tcattcgcca tgagcaggag gcatttgttc tccatgggcg attacagggc gaagagcgcg
     7381 agacggcgat tggcttaacc aaggacaaac agggcgacag caaagtccgc atcgacggta
     7441 ctgacgggca taaagtcgcg gaactggcgc acctgatgcc aatgcagctg ataacgccag
     7501 aagggtttac tttactcaac ggcggcccca aatacagaag agcattcctc gactggggat
     7561 gctttcacaa cgaacccgga tttttcaccg cctggagcaa tctcaagcga ttgctcaagc
     7621 agcgcaatgc ggcgctgcgc caggtgacac gttacgaaca gctacgcccg tgggataaag
     7681 aactgatccc gctggcggag caaatcagca cctggcgcgc ggagtatagc gccggtatcg
     7741 cggccgatat ggccgatacc tgtaagcaat ttctccctga gttttctctg actttctctt
     7801 tccagcgcgg ctgggagaaa gagacagaat atgctgaggt gctggaacgt aattttgaac
     7861 gcgatcgcca gctaacctac accgcgcatg gcccgcataa agcggactta cgcattcgcg
     7921 ccgacggtgc gccggtggaa gataccttat cgcgtgggca gcttaagctg ttgatgtgcg
     7981 ccttacgtct ggcgcaagga gagttcctca cccgtgaaag cgggcggcgg tgtctctacc
     8041 tgatagatga ttttgcctct gagcttgatg atgagcgtcg tgggttgctt gccagccgct
     8101 taaaagcgac gcaatcacag gtctttgtca gcgcgatcag tgctgaacac gttatagaca
     8161 tgtcggacga aaattcgaag atgtttaccg tggaaaaggg taaaataacg gattaaccca
     8221 agtataaatg agcgagaaac gttgatgtcg aattcttatg actcctccag tatcaaagtc
     8281 ctgaaagggc tggatgcggt gcgtaagcgc ccgggtatgt atatcggcga cacggatgac
     8341 ggcaccggtc tgcaccacat ggtattcgag gtggtagata acgctatcga cgaagcgctc
     8401 gcgggtcact gtaaagaaat tatcgtcacc attcacgccg acaactctgt ctctgtacag
     8461 gatgacgggc gcggcattcc gaccggtatt cacccggaag agggcgtatc ggcggcggaa
     8521 gtgatcatga ccgttctgca cgcaggcggt aaattcgacg ataactccta taaagtgtcc
     8581 ggcggtctgc acggcgttgg tgtttcggta gtaaacgccc tgtcgcaaaa actggagctg
     8641 gttatccagc gcgagggtaa aattcaccgt cagatctacg aacacggtgt accgcaggcc
     8701 ccgctggcgg ttaccggcga gactgaaaaa accggcacca tggtgcgttt ctggcctagc
     8761 ctcgaaactt tcaccaatgt gaccgagttc gaatatgaaa ttctggcgaa acgtctgcgt
     8821 gagttgtcgt tcctcaactc cggcgtttcc attcgtctgc gcgacaagcg cgacggcaaa
     8881 gaagaccact tccactatga aggcggcatc aaggcgttcg ttgaatatct gaacaagaac
     8941 aaaacgccga tccacccgaa tatcttctac ttctccactg aaaaagacgg tattggcgtc
     9001 gaagtggcgt tgcagtggaa cgatggcttc caggaaaaca tctactgctt taccaacaac
     9061 attccgcagc gtgacggcgg tactcacctg gcaggcttcc gtgcggcgat gacccgtacc
     9121 ctgaacgcct acatggacaa agaaggctac agcaaaaaag ccaaagttag cgccaccggt
     9181 gacgatgcgc gtgaaggcct gattgcggtc gtttccgtga aagtgccgga cccgaaattc
     9241 tcctcccaga ccaaagacaa actggtttct tctgaggtga aatcagcggt tgaacagcag
     9301 atgaacgaac tgctggcaga atacctgctg gaaaacccaa ccgacgcgaa aatcgtggtt
     9361 ggcaaaatta tcgatgctgc ccgtgcccgt gaagcggcgc gtcgcgcgcg tgaaatgacc
     9421 cgccgtaaag gtgcgctcga cttagcgggc ctgccgggca aactggcaga ctgccaggaa
     9481 cgcgatccgg cgctttccga actgtacctg gtggaagggg actccgcggg cggctctgcg
     9541 aagcaggggc gtaaccgcaa gaaccaggcg attctgccgc tgaagggtaa aatcctcaac
     9601 gtcgagaaag cgcgcttcga taagatgctc tcttctcagg aagtggcgac gcttatcacc
     9661 gcgcttggct gtggtatcgg tcgtgacgag tacaacccgg acaaactgcg ttatcacagc
     9721 atcatcatca tgaccgatgc ggacgtcgac ggctcgcaca ttcgtacgct gctgttgacc
     9781 ttcttctatc gtcagatgcc ggaaatcgtt gaacgcggtc acgtctacat cgctcagccg
     9841 ccgctgtaca aagtgaagaa aggcaagcag gaacagtaca ttaaagacga cgaagcgatg
     9901 gatcagtacc agatctctat cgcgctggat ggcgcaacgc tgcacaccaa cgccagcgca
     9961 ccggcattgg ctggcgaagc gttagagaaa ttggtgtctg agtacaacgc gacgcagaaa
    10021 atgatcaacc gcatggagcg tcgttatccg aaagcaatgc tgaaagagct tatctatcag
    10081 ccgacgctga cggaagccga cctctctgat gagcagaccg ttacccgctg ggtgaacgcg
    10141 ctggtcagcg aactgaacga caaagaacag cacggcagcc agtggaagtt tgatgtccac
    10201 accaatgccg aacaaaacct gttcgagccg attgttcgcg tgcgtaccca cggtgtggat
    10261 actgactatc cgctggatca cgagtttatc accggtggcg aatatcgtcg tatctgcacg
    10321 ctgggtgaga aactgcgtgg cttgctggaa gaagatgcgt ttatcgaacg tggcgagcgt
    10381 cgtcagccgg tagccagctt cgagcaggcg ctggactggc tggtgaaaga gtcccgtcgc
    10441 ggcctctcca tccagcgtta taaaggtctg ggcgagatga acccggaaca gctgtgggaa
    10501 accaccatgg acccggaaag ccgtcgtatg ctgcgcgtta ccgttaagga tgcgattgcc
    10561 gctgaccagt tgttcaccac gctgatgggc gacgccgttg aaccgcgccg tgcgtttatt
    10621 gaagagaacg ccctgaaagc ggcgaatatc gatatttaat ggcgctaacc atgcgagcgt
    10681 gcctgatgcg ctacgcttat caggcctacg aaaatcttgc aatgtattga attttcatga
    10741 ttttgtaggc cggataaggc gttcacgccg catccggcaa gaagctataa gaaaagggcg
    10801 gagatcatct ccgccctttt tatttctgca atccgcaatt atcatcccgc aaatctatac
    10861 tttttgggtc aacacgtaac gctttacagg tgattatcat ggggcttttt gatgaagttg
    10921 tcggtgcctt tctgaaaggc gatgcgggga aatatcaggc tattttaagt tgggttgagg
    10981 agcagggcgg cattcaggtg ctgctggaaa aactgcaaag tggcggctta ggggccattc
    11041 tctcaacctg gctgagtaat caacagggca atcaatcggt tagtggcgag caactggaat
    11101 cggcgctcgg cacaaatgcg gtgtccgatc ttggacaaaa acttggcgtg gataccagta
    11161 cagcttccag tttactggca gaacaattgc cgaagattat tgatgcgctc tcaccgcaag
    11221 gtgaagtgtc tccacaagcc aataacgatc tgctttccgc aggcatggaa ctgctgaaag
    11281 ggaaactctt ccgctaagca aatggggagc atgcaacccg cgttgcatcc cccttttccc
    11341 tcgggtgttg tactgatttt tgagcggaat cgcgttagca tgggtcagga actaatctac
    11401 ctggggaact catggctatc aaactcattg ctatcgatat ggatggcacc cttctgctac
    11461 ccgatcacac catttcaccc gccgttaaaa atgcgattgc cgcagctcgc gcccgtggcg
    11521 tgaatgtcgt gctaacgacg ggtcgccctt atgcaggtgt tcacaattac ctgaaagagc
    11581 tgcatatgga actgccgggc gactactgca ttacttataa cggcgcgctg gtacagaagg
    11641 ccgctgatgg tagcaccgtg gcgcaaaccg ctctcagcta tgacgactac cgtttcctgg
    11701 aaaaactctc tcgcgaagtc ggttctcatt tccacgccct ggaccgcacc acgctgtaca
    11761 ccgccaaccg tgatatcagc tactacacgg tgcatgaatc cttcgttgcc acaattccgc
    11821 tggtgttctg cgaagcggag aaaatggacc ccaataccca gttcctgaaa gtgatgatga
    11881 ttgatgaacc cgccatcctc gaccaggcta tcgcgcgtat tccgcaggaa gtgaaagaga
    11941 aatataccgt gctgaaaagt gcgccgtact tcctcgaaat cctcgataaa cgcgttaaca
    12001 aaggtacggg ggtgaaatca ctggccgacg tgttaggtat taaaccggaa gaaatcatgg
    12061 cgattggcga tcaggaaaac gacatcgcaa tgattgaata tgcaggcgtc ggtgtggcga
    12121 tggataacgc tattccttcg gtgaaagaag tggcgaactt tgtcaccaaa tccaaccttg
    12181 aagatggcgt ggcgtttgct attgagaagt atgtgctgaa ttaatcagtg gacgggcaaa
    12241 cagcataaac gcgtttgccc gcttactgat tattggttta ctgcatatac aaccagaaat
    12301 tgcagcggtg cagtaatgat gtccagcgta acagtcactg gcagtaatgc tgcggaaaca
    12361 ccatccggaa tgcctctggg tgaatagtat tcataaaatc gcactttaaa cggatgatag
    12421 aacgccatca cctttgagta atccatgttt ttatttttct catggattgt gccttttagg
    12481 ttttccagtg acctggtaca gtgttgaaca ccacaggcaa aaccataatg cgttgccagt
    12541 gccttttctt cttcgttatt ccagtggtag taaagcgata ttgtgccggt aaatttcttt
    12601 ttccccgcat ttaaaacaaa tcttgcgtca tcggcaacct gaatattgtg ccggttaagt
    12661 gctggatcat tgagcatctt aacgatgtca tcggcaccat cagtgataag gtaatccagc
    12721 gattccccta caaaaacata gccttttgtt ccattactat cttttgcctg cgatagccca
    12781 gtgatggtgt ctgactgcga ttgatacttt cctgaactac cctttgcggt atgggaaacc
    12841 agagcatacg agatacagcc tgaaagcatc agcgccagtg ggaataaacc ggcagcctta
    12901 aaaaatccct taaaattcaa cttcatgatt aatcctttaa acgagtagct aagccgttga
    12961 aagcatactc ttctttccct cctgtttagc gcctgatatc ccttttcaga tttctgcccg
    13021 acgcatgtca tttttttatg cattgttctt tttgtgatct aaattgtagt acaacaatat
    13081 aagtttgtac tacattacgc acacggcaaa cgcgaacgtc atcacgctgg tactacaaag
    13141 ttgccgcgtt atgcatcgat cggggtaaag tagagaagaa catacagagc acaaggactc
    13201 tccatgactc tcaataaaac cgatcgcatt gtcattacgc tgggtaaaca gatcgttcac
    13261 ggcaaatacg tacctggctc gccacttccg gctgaggcgg agctctgtga agagtttgca
    13321 acctcgcgca acatcatccg tgaggtgttc cgttcgttga tggcgaaacg gctgattgaa
    13381 atgaaacgtt atcgcggcgc gtttgtggca ccgcgtaacc agtggaatta cctcgacact
    13441 gacgtactgc aatgggtgct ggaaaacgac tacgacccac ggcttatcag tgccatgagc
    13501 gaagtgcgaa atctggtgga accggcgatt gcccgttggg cagcagagcg cgcgacttcc
    13561 agcgatctgg cgcagattga atcggcgctg aacgagatga ttgccaacaa tcaggaccgc
    13621 gaagcgttta acgaagcgga tattcgctac cacgaggcgg tgctgcagtc ggtacataac
    13681 ccggtgttac agcaacttag cattgcgatc agttcgttgc agcgggcggt ttttgaacga
    13741 acctggatgg gcgatgaggc caacatgccg caaacgctcc aggaacataa ggcgctgttc
    13801 gatgcaatac ggcatcagga cggcgatgcg gcagagcagg cggcgcttac catgatcgcc
    13861 agctcgacac gaaggttaaa ggaaatcaca tgacagctcg ctacatcgca attgactggg
    13921 gatcgaccaa tcttcgcgcc tggctttatc agggcgacca ctgcctggag agcaggcaat
    13981 cagaagcagg cgtcacgcgc ctgaacggaa aatctccggc tgcggtgtta gcagaagtca
    14041 cgaccgactg gcgtgaagag aatacgccag tggtaatggc aggaatggtc ggcagcaatg
    14101 tcggctggaa agttgctccg tatttatctg ttcctgcccg tttttcgtct attggcgaac
    14161 aattaacgtc tgttggcgac aatatctgga ttattcccgg attatgcgtc tctcatgacg
    14221 ataaccacaa tgtgatgcgc ggcgaagaaa cacaattgat cggcgcgcga actctggctc
    14281 cttcctctct ttatgttatg cccggtacgc attgcaaatg ggtgcaggcc gatagccagc
    14341 aaatcaacga ttttcgcacc gtgatgaccg gtgaattaca tcatttactg ttaaatcact
    14401 cattgattgg cgcaggtttg ccgccgcagg aaaactctgc cgatgccttc gcggctggcc
    14461 ttgagcgcgg ccttaatgcg cccgccatat tgccgcagct ttttgaagtt cgcgcctcgc
    14521 atgtgctggg aacacttccc cgcgaacagg tcagcgaatt tctctctggt ttgttgattg
    14581 gcgcagaggt tgccagtatg cgcgactatg tgacccatca acacgccatc acccttgtcg
    14641 ccggaacatc gctgaccgcg cgctaccagc aagcctttca ggcgatgggt tgcgacgtga
    14701 cggcggtggc gggcgacacg gcatttcagg ctggtataag gagcatcgct catgcagtgg
    14761 caaactaaac ttccgctgat cgccattttg cgcggcatta agcccgacga ggcgctggcg
    14821 catgttggcg cggtgattga cgccgggttc gacgcggttg aaatcccgct gaattcccca
    14881 caatgggagc aaagcattcc cgccatcgtt gatgcgtatg gcgacaaggc gttgattggc
    14941 gcaggtacgg tactgaaacc tgaacaggtc gatgcgctcg ccaggatggg ttgtcagctc
    15001 atcgttacgc ccaatatcca tagtgaagtg atccgccgtg cggtgggcta cggcatgacc
    15061 gtctgccccg gctgcgcgac ggcgaccgaa gcctttaccg cgctcgaagc gggcgcgcag
    15121 gcgctgaaaa tatttccgtc atcggctttt ggtccgcaat acatcaaagc gttaaaagcg
    15181 gtattgccat cggacatcgc agtctttgcc gttggcggcg tgacgccaga aaacctggcg
    15241 cagtggatag acgcaggttg tgctggggcg ggcttaggca gcgatctcta tcgcgccggg
    15301 cagtccgtag aacgcaccgc gcagcaggca gcagcatttg ttaaggcgta tcgagaggca
    15361 gtgcaatgaa aatcaccaaa attaccacgt atcgtttacc tccccgctgg atgttcctga
    15421 aaattgaaac cgatgaaggc gtggtcggtt ggggcgagcc cgtgattgaa ggccgcgccc
    15481 gtacggtgga agcggcagtt cacgagctgg gtgactattt gattggtcag gatccttcgc
    15541 gcatcaatga cttatggcaa gtgatgtatc gcgccggatt ttatcgtggc ggtccaatcc
    15601 tgatgagcgc cattgccggg atcgaccagg cgttatggga tatcaaaggc aaagtgctga
    15661 atgcgccggt ctggcaactg atgggcggcc tggttcgcga caaaattaaa gcctacagtt
    15721 gggtcggcgg cgatcgtccg gcggatgtta tcgacggcat taaaaccctg cgcgaaatcg
    15781 gcttcgatac cttcaaactg aacggttgtg aagaactggg gctaattgat aactcccgcg
    15841 cggtagatgc ggcagtcaac accgtggcac aaattcgtga agcttttggc aatcagattg
    15901 agtttggtct tgatttccac ggtcgcgtca gcgcgccaat ggcgaaagtg ctgattaaag
    15961 aactggagcc gtatcgcccg ctgtttattg aagagccggt gctggcggaa caagccgaat
    16021 actacccgaa attggcggca caaacgcata ttccactggc ggcaggtgag cgcatgttct
    16081 cacgtttcga ttttaaacgc gtgctggagg caggcggtat ttcgattctg caaccggatc
    16141 tctcccatgc aggcggtatt accgaatgct acaaaattgc tggaatggca gaagcctatg
    16201 atgtgaccct tgcgccgcac tgtccgctcg gaccgattgc actggcggct tgcctgcata
    16261 tcgactttgt ttcctataac gcggtacttc aggaacaaag tatgggcatt cattacaaca
    16321 aaggcgcgga gttactcgac tttgtgaaaa acaaagaaga cttcagtatg gttggcggct
    16381 tctttaaacc gttaacgaaa ccgggcttag gtgtggaaat cgacgaagct aaagttattg
    16441 agttcagtaa aaatgccccg gactggcgta atccgctctg gcgtcatgaa gataacagcg
    16501 tagcagagtg gtaattcctg ccacgtaagc ccctcatcgg gcactaaaac agcaatacaa
    16561 aaatataacc ctctgtaaat tacagggcat ggtgagcggc ttcgctatgc ccaaaatctg
    16621 gagaaagctc gctatggata ttcccgttaa tgcagcaaag ccggggcgtc ggcgttatct
    16681 gacgctggtg atgatcttta ttacggtggt catttgttat gttgaccgcg ctaacctggc
    16741 cgtggcttcc gcccatattc aggaagagtt cggcattacg aaagcggaaa tgggctatgt
    16801 attttcggcc ttcgcctggc tttatacgct atgccagatc cccggcggtt ggtttttaga
    16861 tcgcgtaggt tctcgcgtga cttattttat tgcgatattt ggttggtcag tggcgacttt
    16921 attccagggc tttgccacgg gattaatgtc attaattggt ctgcgtgcga taaccggtat
    16981 tttcgaagcg cccgctttcc cgaccaataa ccggatggtg accagctggt tcccggaaca
    17041 tgaacgcgct tccgccgttg gtttttatac gtctggtcag tttgtcggtc tggcgttttt
    17101 gactccgctg ctgatctgga ttcaggagat gttgagctgg cactgggtgt tcattgtcac
    17161 tggtggtatc ggcattatct ggtcgctgat ttggtttaag gtttatcagc cgccgcgcct
    17221 gaccaaaggt atcagcaaag ctgaactgga ttacattcgt gatggcggcg gtctggtgga
    17281 tggtgatgcg ccggtgaaga aagaggcgcg tcagccgtta acagccaaag actggaaact
    17341 ggtgttccat cgtaaactga tcggcgttta tcttgggcaa tttgcggtgg cttctacact
    17401 gtggtttttc ttaacctggt tcccgaacta tttaacccag gaaaaaggaa tcacggcgct
    17461 gaaagcaggc tttatgacca cggtgccatt cctcgcggcg tttgtcggcg tcctgctctc
    17521 tggctgggtc gcggatctgc tggtacgtaa gggcttttca ctgggctttg cgcgtaaaac
    17581 gccgattatc tgcggcttgc tgatctccac ctgcattatg ggcgctaact acaccaacga
    17641 tccgatgatg attatgtgcc tgatggcgct ggcattcttc ggcaacggtt ttgcttcgat
    17701 tacctggtcg ctggtctctt ctctggcacc gatgcgcctg attggtttaa ccggcggcgt
    17761 gtttaacttc gccggtggtc tgggcggcat caccgttccg ctggtggtgg ggtacctggc
    17821 gcagggttac ggtttcgcac ctgcactggt ttatatctcc gccgtcgcgt tgattggcgc
    17881 gctctcttac atcctgctgg tgggcgatgt gaagcgcgtt ggctaatctt tcaactgtgg
    17941 aatatgcgcc ataccactgc gcataatccg ctttcttaaa gccggtgcgg tcaggcatcg
    18001 gctttttact atcttgccga agagttttaa acgcagtttg cgggtggcgc gccagtaagc
    18061 ggtattgagc ttctctggct gcttcagtaa caccgaacgc agaatgtctg cgctatccag
    18121 cgcatagcta atcccctcca gcgaactggc gctgataaat cctgccgctt cgccaatcag
    18181 aaaggcgttg tccttaccgc agacaaaatc ctgccagcgt gagggaaaca gcaccgtgca
    18241 tttttcgctt ttcaccgcct taccaaactg gaactgaaag gcgctcattt tctctttcag
    18301 cgtcgtgaaa cgcgtctgac cgtctttcat tggataagca ccgccaaaga taaaataacc
    18361 gtctttgctg atactccatg aataacagtt agttatcgaa ttatcaaaga tgcaggagta
    18421 gaacggcacc ggatgtttct ctgcgaacca ctgctggata gcgacatatt tacggatttg
    18481 atgatccggg tagagatgtc gccgcaccat cgagtttgcg ccatcggcac cgaccagata
    18541 gcgggcagta atatgttgct cccagccgtc tgcacgaaaa atgacatgcc atttatcatc
    18601 ctcacgccag atttttcgac acaggctgtc gtgataaatc tcaacgctgg cggggatcag
    18661 cgatttcatc cacaagtcga aagcatggcg attaatattg atatagcttc gctggtagtt
    18721 acgcgtgagc gatgcggcga cgtcgacagt tttgacgctg aaaatctgcg ggttggcgat
    18781 cacatcgaca ggaagcgtca gtccatcgcg aataaaagat cgctgcgcgt ccggtgccag
    18841 cagaccgccg caaggtttgc tgaaaccttc agtaccacac tggtgctttt tatccagcgc
    18901 gatcacctgc attttgcctg ctaattttcg cgccaacgcc gatcctgccg ggccgaggcc
    18961 aataatcgcc acgtcgaaat gttccatctt ttcactcaca ttcatcacga tggcaaaaga
    19021 ctattgtctg aaagtgaagt ccttgtgaag tatgatggcg ggacccgttt tgtatcagga
    19081 ccgcgccatg aaacagataa cctttgctcc ccgtaatcac ctgctcacca ataccaatac
    19141 ctggacgccc gacagccagt ggctggtatt tgacgtgcgt ccttctgggg cgtcgtttac
    19201 cggcgagacc attgagcgtg tgaatatcca taccggcgag gtcgaggtta tctatcgcgc
    19261 gtcacagggc gcacacgtcg gcgtggtgac cgttcatccg aagtcagaga aatatgtttt
    19321 catccatggc ccggaaaatc ctgatgaaac atggcattac gatttccatc atcgacgcgg
    19381 agtcattgct gaaggcggca aggtgagcaa tctcgatgca atggatatta ccgcgccgta
    19441 tatgccagga gcgctgcgcg gcggcagcca tgtgcatgtc tttagcccga acggtgaaag
    19501 ggtgagcttt acctataacg accatgtaat gcatgaactc gatccggcgc tggatttgcg
    19561 aaacgtcggt gttgctgcgc cgtttggccc ggtcaacgta caaaagcagc atccgcgtga
    19621 atacagcggt agccactggt gcgtgctggt gagtaaaacc acgcccacgc cgcagcctgg
    19681 cagcgatgaa atcaatcgtg cttatgaaga aggatgggta ggaaatcacg cgctggcgtt
    19741 tattggcgac acactttcgc caaagggcga gaaagtgccg gagctgttta tcgttgagtt
    19801 accgcaagat gaagctggct ggaaagcggc aggtgatgcg ccgttaagtg gaacggaaac
    19861 caccctgccc gcgccaccgc gcggcgtcgt gcagcgacgt ttaaccttta cccaccatcg
    19921 ggcttatccg gggttagtca acgtcccgcg ccactgggtg cgctgtaatc cgcagggtac
    19981 gcaaatcgcg tttttaatgc gtgatgataa cggcattgtg caactgtggc ttatctcgcc
    20041 acagggcggc gagccgcgcc agttaaccca taacaaaacg gatattcagt ctgcatttaa
    20101 ctggcatccg tcaggagaat ggttgggctt tgtgctggat aatcgaattg cttgtgccca
    20161 tgcgcaaagc ggcgaggttg agtatttaac cgaaaaccac gccaatccac cttctgcgga
    20221 cgccgaggtc ttctcgccgg atggtcaatg gctggcgtgg atggaaggtg gccagctgtg
    20281 gatcaccgaa actgatcgct aaatcagtta tcaggcattc tggccggtgg aataaccgca
    20341 ttggtggctt gcgcgttggc ttcgcttttt tcaacgcgtg agcgcaccga gctgtctttg
    20401 cggaacacat cccacggcag taaaagcgtg tccatcacag cggtaaacgg catatcgaga
    20461 atcgccagcg acttagtgcc ccagttggtt tcatcgtcgc caatcatggt tgcgctggcg
    20521 cgggtgccgg gatacgttcc ttctttccct ccggtatggg acatcacgct ggagcatcct
    20581 ccgagtaatg tcattccgct gcatatcatg aacgctaaca acacatttct tatcataata
    20641 aattcatctg ttgatcgtgg gtgttggcct gatgagttat agcgatccct tgctgaaaat
    20701 aacatcatca ttacgtcgca ctgtggcggc tatcgcactt taacgtttcg tgctgccccc
    20761 tcagtctatg caatagacca taaactgcaa aaaaaagtcc gctgataagg cttgaaaagt
    20821 tcatttccag acccattttt acatcgtagc cgatgaggac gcgcctgatg ggtgttctgg
    20881 ctacctgacc tgtccattgt ggaaggtctt acattctcgc tgatttcagg agctattgat
    20941 tatgcgtaac tttgatttat ccccgcttta ccgttctgct attggatttg accgtttgtt
    21001 taaccactta gaaaacaacc agagccagag taatggcggc taccctccgt ataacgttga
    21061 actggtagac gaaaaccatt accgcattgc tatcgctgtg gctggttttg ctgagagcga
    21121 actggaaatt accgcccagg ataatctgct agtggtgaaa ggtgctcacg ccgacgaaca
    21181 aaaagagcgc acctatctgt accagggcat cgctgaacgc aactttgaac gcaaattcca
    21241 gttagctgag aacattcatg ttcgtggcgc taacctggta aatggtttgc tgtatatcga
    21301 tctcgaacgc gtgattccgg aagcgaaaaa accgcgccgt atcgaaatca actaattccc
    21361 taaggccgcc tggcgcggcc tgacatctcc atgctcgccg ttagggagca tatgcgaatc
    21421 ttcggatttg caggtactta ctcgcttctt agaaggagaa atgactatgc gtaacttcga
    21481 tttatcccca ctgatgcgtc aatggatcgg ttttgacaaa ctggccaacg cactgcaaaa
    21541 cgccggtgaa agccagagct tcccgccgta caacattgag aaaagcgacg ataaccacta
    21601 ccgcattacc cttgcgctgg caggtttccg tcaggaagat ttagagattc aactggaagg
    21661 tacgcgcctg agcgtaaaag gcacgccgga gcagccaaaa gaagagaaaa aatggctgca
    21721 tcaagggctt atgaatcagc catttagcct gagctttacg ctggctgaaa atatggaagt
    21781 ctctggcgca accttcgtaa acggtttact gcatattgat ttaattcgta atgagcctga
    21841 acccatcgca gcgcagcgta tcgctatcag cgaacgtccc gcgttaaata gctaactagc
    21901 tattctcttt gccccgccat tctggtgggg ctttttttgt cttacttact caacaatgcg
    21961 ttaaatttcg actgtttaag atatttcggc acgttctttc ccgtgataat gtgcgctata
    22021 aggcaaatct gcttcaccga ttcagccata atccgtagca attaaaaatg ttatctcaag
    22081 agaaatggac gatgagtgat atagcattaa cggtcagtat tctggctttg gtggcagtcg
    22141 tcggtttgtt tatcggcaat gtcaaatttc gcggcatagg attaggtatt ggcggcgtgc
    22201 tgtttggtgg gatcatcgtc ggccattttg tttctcaggc ggggatgaca ttaagtagcg
    22261 atatgctgca tgttattcag gaatttggcc tgatcctgtt cgtttatact atcgggattc
    22321 aggtagggcc gggcttcttt gcctcattgc gcgtctccgg attacgcctc aacctgtttg
    22381 ctgttctgat cgtcatcatc ggtggtctgg ttaccgccat cctgcataaa ctgtttgata
    22441 tgccactgcc ggtagtgctg gggattttct ccggtgcggt taccaatacg ccagcgctgg
    22501 gggcagggca gcagattttg cgcgacttgg gtacaccaat ggaaatggtc gatcagatgg
    22561 ggatgagtta cgcgatggcg tatccattcg gcatttgcgg gattttgttc accatgtgga
    22621 tgttgcgggt tattttccgc gtcaatgtcg agacagaagc tcagcagcac gagtcttcac
    22681 gcaccaatgg cggcgcgctg atcaagacta tcaatattcg cgttgagaac cctaacctgc
    22741 atgatttagc cattaaagat gtaccgattc tcaacggcga caaaattatc tgctcgcgtc
    22801 tgaaacgcga agaaacccta aaagttcctt cgccagatac cattatccaa ctgggcgatt
    22861 tgctgcatct ggtggggcag ccagcggatt tacataatgc gcaactggtg attggtcagg
    22921 aggtcgatac ttcgctgtcc acgaaaggca ctgatttgcg cgtcgagcgt gtggtggtca
    22981 ccaatgaaaa cgtgctcgga aaacgtattc gcgacctgca ctttaaagaa cgctatgacg
    23041 ttgttatctc gcgcctgaac cgtgccgggg tcgaactggt cgccagtggc gatatcagcc
    23101 tgcagttcgg cgatatcctc aatctggtgg ggcgtccgtc cgcaattgat gccgttgcca
    23161 atgtgctggg gaatgcgcag caaaaactgc aacaggttca gatgctgcca gtgtttattg
    23221 gcatcgggct tggcgtattg ttaggttcta ttcccgtctt tgtgcctggg ttcccggccg
    23281 cgttgaaact ggggctggcg ggcggtccgc tgattatggc gttgatcctc gggcgtatcg
    23341 gcagtatcgg caagctgtac tggtttatgc cgccaagtgc caacctcgcg ctgcgggagc
    23401 tggggatcgt gctgttcctc tcggtcgttg gtctgaaatc tggtggagat tttgtgaata
    23461 ccctggtcaa tggcgaaggg ctaagctgga ttggttatgg tgccctgatc accgccgttc
    23521 cgctgattac tgttggcatt ctggcgcgga tgttagccaa aatgaattac ctgaccatgt
    23581 gcgggatgct ggcaggttcc atgaccgatc ctccggcgct ggcgtttgct aataatcttc
    23641 atccaaccag cggtgcggcg gcgctctctt acgccactgt ctatccgtta gtgatgttcc
    23701 tgcgcattat caccccccaa ttactggcgg tgctcttctg gagtatcggt taactctttt
    23761 ccgggtggag tcgccgtaaa tggtactcca cctggtattc gctggcattt ctgaacatca
    23821 ctgaataatt caaaaactcc ccgctctcgc tatatgacaa cgaggtgatc cgcagcagtg
    23881 gcgtgtgttc cgctaccttc atttgccgcg ccagcagtct atcggcgagc actggcgtca
    23941 ggctttcata attaccgcca atcaaaatcc cgcactcttg ttcaatatat tcaaacttcg
    24001 acccttccag atgttgcagc gaaagattac ggaacagttt taccggcata tagctgtctt
    24061 ccagcatcag cggtttccct tccacaaaac gaacgcgacg ggagaagtag atctgctcgt
    24121 tgatttgaat ccgtaactgg ctggcaatgg ccggaggcgc aggcatgatt tcaaaaatca
    24181 gcacctggct ggtgaccgtt tttccctgcc gttttaacac ctccaccagc ccggtcaggc
    24241 tggcggtttg atgcagtaca tctttgcgca ccaggtaagt gccgctgccg tggcggcgga
    24301 ccaccaatcc ccacgctacc agcaggtcaa tggctttacg gatggtcatc cgcgataccg
    24361 caaattcttc cgccaacttt ttttcaccgg gaagaaggct atttagcgtg aaatctgcgg
    24421 aattaagtcg aattcttaac cgctccgcaa tgcttttgta gatcatctct agatctcttt
    24481 tttgcctgca agcacgggta atgcgcccac aagttctgct taatcgattg aaaaaacatc
    24541 ttttttttaa agatgtgttc gatgctgtgt acctgtgctc acagatgtct actttttcgc
    24601 gaaaacgtag atctctaccg cccaacgaaa agcatgaaag cgatcacgaa tcccattggg
    24661 tcgtcatgtt ctgccgatcg catcttccta tcctcgctcc aggcctgccg cataaccaat
    24721 caggcttcct acttacagaa ttgagaaaag aggatgtgga aatgctcagt caaattcaac
    24781 gctttggcgg cgcgatgttc acgccagtgc tgctgtttcc cttcgccggg attgtggtgg
    24841 gtcttgccat cttgctgcaa aacccgatgt ttgtcgggga atcactgacc gatccgaaca
    24901 gtttattcgc gcaaatcgta cacattattg aagagggcgg ttggacggtg ttccgtaata
    24961 tgccgctgat ttttgctgtc ggtttaccca ttggccttgc taagcaagcg caggggcgtg
    25021 cttgtctggc ggtgatggtg agtttcctga cctggaacta tttcatcaac gcgatgggaa
    25081 tgacctgggg aagctacttc ggcgtcgatt tcactcagga cgcggtggca ggtagcggtc
    25141 tgacaatgat ggccgggatt aaaaccctcg ataccagcat tatcggcgca attatcattt
    25201 ccggcattgt gacggcgctg cataaccgtc tgttcgataa aaaactgccg gtttttctcg
    25261 gcattttcca ggggacgtct tatgtggtga ttatcgcctt cctggtgatg atcccctgtg
    25321 cctggctgac gttgctcggc tggccaaaag tacaaatggg gattgaatct ctgcaagcgt
    25381 tcctgcgttc ggcgggtgcg cttggggtgt gggtttacac cttcctcgaa cgtattctga
    25441 tcccaaccgg tttacaccac ttcatctacg gaccgtttat ctttggtccg gcagctgttg
    25501 aaggcggcat tcagatgtac tgggcgcagc atctgcaaga gttcagtctg agcgccgagc
    25561 cgctgaaatc gttgttcccg gaaggcggtt ttgccctgca cggtaactca aaaatctttg
    25621 gtgccgtggg catttcttta gcgatgtact tcactgccgc accggaaaat cgggtaaaag
    25681 tggcgggctt gctgattccc gcaaccttaa ccgccatgct ggtgggaatt accgaaccgc
    25741 tggaatttac cttcctgttc atttcaccgt tgctgtttgc ggtacacgcc gtgctggcgg
    25801 cctcaatgtc gaccgtgatg tatctctttg gtgtggtggg caacatgggc ggaggtctga
    25861 ttgaccaggt tttaccgcaa aactggatcc cgatgttcag caaccacgcg gatatgatgc
    25921 tgacccaaat cgccattggg ttgtgcttta ccctgctgta cttcgtggtt ttccgcaccc
    25981 tgattctgca gttcaacatg tgcacgccgg gacgtgaaga tgcggaagtg aaactctact
    26041 caaaagccga atacaaagcc tcgcgaggcc aaaccaccgc ggcagagcca aaaaaagagc
    26101 tggatcaggc tgccggtatt ctgcaagccc tgggcggggt cggcaatatc tccagcatta
    26161 acaattgcgc gacgcgtcta cgcattgcac tgcatgacat gtcacaaacg ctggatgacg
    26221 aagtctttaa aaagctggga gcgcacggcg tcttccgtag tggcgatgcc attcaggtga
    26281 tcattggtct gcatgtatcc caattgcgtg aacagctcga tagcttaatt aattctcatc
    26341 aatcagcaga aaatgttgcc attacggagg cagtataatg accaaattct cagtggttgt
    26401 cgcaggcggt ggaagcacct ttacgccagg catcgtgttg atgctcctgg cgaatcagga
    26461 ccgtttcccg cttcgtgcac tgaaatttta tgataacgat ggcgcgcggc aggaagtgat
    26521 tgccgaagcc tgtaaagtca tccttaaaga aaaagcgccg gacattgcgt ttagttacac
    26581 caccgatcct gaagtagcgt tcagcgacgt tgattttgtc atggcgcaca tccgcgtcgg
    26641 caagtacccg atgcgcgaac tggatgaaaa aatcccactg cgccacggtg ttgttggtca
    26701 ggaaacttgc ggacccggcg gaatagcgta cggcatgcgt tccattggcg gcgtcctgga
    26761 actggtggat tatatggaaa aatattcacc aaacgcctgg atgctcaact actccaaccc
    26821 ggcagcaatt gtcgcagaag ccacgcgtcg tctgcgcccg aatgcgaaaa tcctcaacat
    26881 ctgtgacatg ccaatcggta ttgaaagccg gatggcgcaa attgttgggc tgcaagatcg
    26941 caaacagatg cgcgtgcgct actacggcct gaaccactgg tggtcggcga tatcccgcag
    27001 tttcagaaag ggttaatgag tcagcaagtg gcggtggaaa aactggtcgt ggacgcctgg
    27061 gaacagcgtt catatcagca cctgtggcag gcgattacgt tgtcgaaaac ggtaccgagc
    27121 gcctcggtcg ccaaagctat tctggatgag ttgctggagg ccaacaaagc gtactggcca
    27181 gagttacgtt aattgtgtgg actggcatcc gttgggtgct ggtccgtatt ctttagcacg
    27241 gctgacggcg cataaccaaa tttatgttta aaggctttgc tgaaatgaaa ggagtcgaag
    27301 aagttgagcg tatcagcgac ttcgccaacc gagtttcctt gttgctttaa tagcgatagc
    27361 gccagatcca gacgcgcatc gaggtaatat tctttcggcg tcttgccggt atagcgtaaa
    27421 aacagacgac gcaaccaggc ttcgctacag gggatcgtgg cagccatatc agctacgctc
    27481 cagcgttgtt gcagactggc atgtaaagtg gcaattaatt tttcaatttg ccgccgttgt
    27541 ggatcttttt taccatctgc gtacataaga caaatccact gataaataat tttcgtcaga
    27601 aaagcgactg ccagattatt ttttattgct tctggtgaag ttattaactc agcaacttcg
    27661 gtgagttcct gattataaat ttcgccatta taaataacgc tttgctgacc aacgggaata
    27721 tccatcatac tggtgggggt aaattccatc cagtactgtt cccagactaa accttcacag
    27781 tgataagagt gaatatccat tggctttaag aatataatac agttgccatg cagtgttatt
    27841 tgttcgccat tctttaaaag aattttccca catccttgta cggtatacac cgcaacccat
    27901 gagttggcga taagcggttt cttcttgtcc cgcggccaga tcacccggta atgctcgtcg
    27961 gcgtaggtat gccagagcga gatcagagaa attccgctgg agatcacatt ttcgttaatc
    28021 aataagggaa tattgtacgg agtttcgttt tttacatctg aactttgcaa ttttccattc
    28081 attttaatat ccctccggcg aattatgctc acaacctgaa cttaacagaa aacaatccgt
    28141 tcagggatac gacgtttgta ttttaagaat ctgactgcct gacccgacgc attttacctc
    28201 tccctatatt catgcgtccg ggacataaac ataaataagg gctatgagat gaattcgtta
    28261 caaatcttga gttttgtcgg ttttacgctg ctggtggcgg tgatcacctg gtggaaggtc
    28321 cgcaaaacag ataccggatc gcaacaaggc tattttcttg ccggacgttc actaaaagcg
    28381 ccggttattg ccgcttcgtt aatgctaacc aacctttcca cggaacaact ggtcggcctt
    28441 tccgggcagg cctacaaaag cggcatgtcg gtgatgggct gggaagtgac ttcagcggtg
    28501 acgctgatct tcctcgcgct aatcttttta ccgcgctatc tgaagcgcgg cattgccacc
    28561 atccccgatt ttctggagga acgttatgat aaaacgacgc gtattatcat cgacttctgc
    28621 ttcctcattg ccaccggcgt ctgctttctg ccgattgttc tctactccgg cgcgttggcg
    28681 ctcaacagcc tgtttcacgt cggggaatcg ctacagattt ctcacggtgc ggctatctgg
    28741 ctactggtaa ttttgcttgg tctggcggga attttgtatg cggtgatcgg cggactgcgc
    28801 gcaatggcag tggcggactc catcaacggt attgggctgg ttatcggcgg gttgatggtg
    28861 ccggtatttg gcctgatcgc gatgggcaag ggcagcttta tgcaaggcat tgagcaactc
    28921 accaccgttc acgccgagaa attaaactca atcggtggcc cgaccgatcc cttgccgatt
    28981 ggcgcggcat ttaccggttt gattctggtg aacacctttt actggtgtac aaatcagggc
    29041 atcgtgcaac gcacgctggc gtcaaaaagc ctggcggaag ggcaaaaggg ggcgctgtta
    29101 acggcggtgc tgaaaatgct cgacccgctg gtactggtgc tgccagggtt gattgcgttt
    29161 catctgtatc aggatttacc gaaagccgac atggcctacc cgacgctggt caataacgtt
    29221 ctgccagtgc caatggtggg tttcttcggc gcggtgttat ttggtgcggt gatcagtacc
    29281 ttcaacggct ttctgaatag cgccagtacg ttattcagta tgggtattta ccgtcgcatc
    29341 attaaccaga atgccgagcc gcagcagctg gtcaccgtcg ggcgcaaatt tggtttcttt
    29401 atcgctatcg tttcggtgct ggtcgcgccg tggatcgcca acgcgccgca ggggctgtat
    29461 agctggatga aacagctcaa cggcatttac aacgtgccgc tggttaccat catcattatg
    29521 ggctttttct tcccgcgcat cccggcgctg gcggcaaaag tggcgatggg gattggcata
    29581 atcagctaca tcaccatcaa ctatctggtg aagttcgact tccatttcct ctatgtgctg
    29641 gcctgtacgt tctgcatcaa cgtggtcgtg atgctggtga tcggttttat caaaccgcgc
    29701 gccacgccgt tcaccttcaa agatgcgttt gcggtggaca tgaaaccgtg gagaaacgtc
    29761 aagatcgcgt caattggcat cctgttcgcg atgattggcg tctatgccgg gctggctgaa
    29821 ttcggcggct acggtacgcg ctggttagcg atgatcagtt atttcattgc cgccgtagtg
    29881 attgtctacc tgatttttga cagctggcgg catcgtcacg acccagccgt aacctttact
    29941 cccgacgcga aggatagcct atgaaacgcc ccaattttct gttcatcatg accgataccc
    30001 aggccaccaa tatggtcggt tgctatagcg gtaaaccgct gaatacgcaa aatattgata
    30061 gtctggcggc ggaaggtatt cgctttaatt ccgcctacac ctgttcaccg gtttgtacgc
    30121 ctgcacgcgc cggactattt accggtatct acgctaacca gtccggcccg tggaccaaca
    30181 acgtcgcgcc aggcaaaaac atctccacta tggggcgcta ctttaaggat gccggctatc
    30241 acacctgtta catcggcaaa tggcatctcg acggtcatga ctatttcggc actggcgagt
    30301 gtccgccgga gtgggacgct gattactggt tcgatggggc gaactacctt agcgaactga
    30361 cggaaaaaga gatcagcctg tggcgcaatg gcctaaacag cgttgaggat ttacaggcga
    30421 accatatcga cgaaaccttc acctgggcgc accgcatcag caatcgggca gtagattttc
    30481 tgcaacagcc cgcgcgcgcc gaggaaccct tcctgatggt ggtttcgtat gatgagccgc
    30541 atcacccgtt cacctgtccg gtggagtatt tagagaaata cgctgatttt tactacgatc
    30601 tgggcgagaa agctcaggat ggcctggcga acaaaccgga acatcaccgc ttatgggcgc
    30661 aggcgatgcc atcgccagtc ggtgatgacg ggctttatca ccatccgctc tattttgcct
    30721 gtaatgactt tgttgatgac caaatcggac gggtcatcaa tgccttaacg ccagagcaac
    30781 gtgaaaatac gtgggtcatt tatacttccg atcacggcga aatgatgggc gcacataagc
    30841 tgatcagtaa aggagcggcg atgtatgacg acatcacccg tattccgctg atcatccgtt
    30901 cgccgcaagg ggagcggcga caggtcgata cgccagtcag tcatatcgat ttactgccga
    30961 caatgatggc gctggcagat attgaaaaac cagagattct gccgggggaa aatatccttg
    31021 ccgtgaaaga gccacgcggt gtaatggtgg aatttaaccg ctacgagatt gagcatgaca
    31081 gctttggcgg ttttattccg gtgcgttgct gggtgacgga tgactttaaa ctggtactca
    31141 acctcttcac cagtgatgaa ctttacgatc gccgtaatga cccaaatgaa atgcataacc
    31201 tgatcgatga tatccgtttt gcagacgttc gcagcaaaat gcatgacgcc ttattggatt
    31261 acatggacaa aattcgcgat ccgttccgca gttaccaatg gagcctgcgt ccgtggcgta
    31321 aagatgcact gccgcgctgg atgggggcat ttcgtccacg cccacaagat ggctattcgc
    31381 cggtggtacg tgactatgac accggcctac cgacgcaagg agtgaaagtg gaagagaaaa
    31441 aacagaagtt ctgacgataa tgcccgctga tgaacacccg gcgggcatta ttcaggctgc
    31501 ttaaaccagt gaaccgccgg tcaggaagga aaaaatcagg aatatcagca aaatgctgca
    31561 cacgatgccg ataccaaaaa gcaaggtatg gaaagccagc gtcccgccgc caaaaaccag
    31621 cgcgccgcga atgccccagc gattttcctt acgcataccg ccaataaatg cgcaaagtag
    31681 cgcagcaatg gccagtgcga taccgctttg atcgaggatt ttatcgacat ctacgtcctc
    31741 ttttttctcc acggtggtaa ttttttcgcc ttttattccg gcgagaagac ctcttttaat
    31801 ttcagccgtt ttgtccgcca cgatgctttc aaacgttggc ggtggtgcgg aaaaagggcc
    31861 gaatgaaaag tggataatcc ccatcattaa agcgatggca ccgaacagca tgcccaatga
    31921 actgatttta ttttgagcta tgatacccat ctgacctcct tgtagcggat ggcgaaagca
    31981 tatcaggaaa cgggcggacg aaattcttaa aaaaaaaccg ctaaaggtag agcgttagtg
    32041 aaattgcaaa ggagcaacaa tgaaaatttc ccgccttgga gaagcgcccg attaccgctt
    32101 ctcgctggct aatgagcgta cttttctggc gtggatccgt accgcactag gatttctggc
    32161 ggcgggggtt gggcttgatc aacttgcgcc agatttcgcc acgcccgtca ttcgtgaact
    32221 gctggcgttg ttgttgtgtc tgttttccgg cgggttagcg atgtatggct acctgcgctg
    32281 gttacgcaat gaaaaagcga tgcgtctgaa agaggacctg ccctacacca acagcttatt
    32341 aatcattagc ttaatattga tggtcgtggc ggtgatcgtt atgggactgg tgttgtatgc
    32401 cggatagccg caaagccaga cgcattgccg acccaggact gcagccggag cgcacatcac
    32461 tggcgtggtt tcgtaccatg ctgggctacg gcgcgttgat ggcgttggct atcaaacaca
    32521 actggcacca ggcgggcatg ttattctgga tttcgattgg cattctcgcc atcgtggcgc
    32581 tgatcctctg gcactacacc cgtaatcgca atttaatgga tgtcacgaat agcgattttt
    32641 cccaatttca cgtagtccgt gacaaatttt tgatctccct cgcggtgtta tctctcgcaa
    32701 tactgtttgc tgtaacgcat atacatcaac ttatcgtatt tattgagaga gtcgcatgac
    32761 aggaagtcag gtcattgatg ctgaagagga caggcataaa ctggttgtag agtacaagga
    32821 tgctctgcaa cctgccgatt tttatcacaa tttcaaacag cggggcatcc gctccgtaca
    32881 gctcattccc tatcttgaat ttgatgaccg gggcgatctg acggccgctt cggtgacagc
    32941 agagctgtgg gggaaatttt taattgccct gttcgagtgt tgggtgcggg cagatatcag
    33001 tcgtatctcg attgagcttt ttgacgccac cttacaaaaa tggtgtggca gcgaaaaccc
    33061 gcaaccgcgc cgtgactgcc aggcgtgcga ctggcatcgc ctgtgcccgc acgctcgcca
    33121 ggagacgcca gacagcgtgc tttgtgcggg ctatcaggcg ttttattctt actccgcacc
    33181 gcatatgcgg gtgatgcgtg atctgataaa gcagcatcgt tctcccatgg agctgatgac
    33241 gatgctgcgg tgacgtgcgc ttaaacgggc tgcccctgat gcgacatccg cgtcgccagc
    33301 ggcagccagc acagcacgat caacaatccc attaaggtca tcaacaaccc caggctgccc
    33361 tgaccggttt gcggcaacat cgcagagagc gacgccagca cgccggaacc aatgttttgc
    33421 agaccgccga ccagcgcgcc agccgtgccc gccaggaagg ggaacggctc catcgcgccg
    33481 ctggtcgcca gcggaaacag catcccggca ccgaaaaaga acagcgcggc gggaacgagc
    33541 agcgtccaga cattcatcac gccaaaccag tcggggatcc acatcagcaa gccagccagc
    33601 aggcagcaga taaccgactg ccacattaac gtggagaagc gtttattggg acgtccggca
    33661 aaccatgcgc caaaaaacgc tgccggaatc ggcagaataa acaaaatact gaccgtcata
    33721 ctgctcagcc ctaacaccgc gcccatcagc acgccggagc aggcttcaaa ggcggcaatc
    33781 ccggccagac cgccaatcag catcagcaaa taacagttaa aaccgctgtt accgaaaagc
    33841 gttttataac tggtaagcag gcgcgtgcgc ggtgcatcga ccggacgcgt ttccggcatc
    33901 cagcgggcca tactgaaggt cacaccagca caaagaacca acaaaaagag ataacaggcg
    33961 cgccagttcc acatcgtatc cagcagaccg ccgattagcg gtgcgagcaa cggactgacg
    34021 agaatcccca tgtttaacag gctgttagca tggcgcaact gtgtccgttc atataaatct
    34081 cgcggcaaag tacgcgccat tacgccgcca acgccggtgc ccatcccctg catcgcgctg
    34141 gcggcaatca acaccgtcaa actggaggtc gtgaccgcga ccagcgttgc cagcataaaa
    34201 atggacattc cgacgaggat caccggtcgg cggcccacgc ggtcggaaat cgggccataa
    34261 aacagctgtg agacaccgta agtcagcaga taagcgccca ttacgctctg caccgcccct
    34321 tcacggacgt tgagatcgcg cgccatatcg gcaatagctg gaatataaat ggtttgcgcc
    34381 atctgaccga cggccacgag taataccaac atcaataaca aattgacgtt tctttgcctt
    34441 ttcattatca cggatgcttt tataaaaata attagaataa gtcactacgc attcccataa
    34501 atgcgggaga ggaatctatc acattgatga atgttagcca gatatacgcc aggaatgggg
    34561 aattgtttag cggcagaata tgtaaacaaa agcggcaata aatgttgccg ggagggatta
    34621 cgtgcgaact gtaaccctct ccccaaaggg gcgaggggga aaccaccgtt ccctttcaga
    34681 aaatgagcta aacattgcac gggacggtcc cctcgcccct ttggggagag ggctagggtg
    34741 aggggcacag gcagcgcagg tgcaaacatt ctgctggtag aagaagcccc caactccaac
    34801 ccgcacgcta aatataaaag gggagcggtt acccgctccc ctttggtgcg acttgaatct
    34861 gaattacttc aggtatttca gaacagcatc aagcagttgc agtgctgcaa caatgagttt
    34921 gaggataaga atggcgatat ccaccaggct catacgcgtc tcctgtggtt caggagcact
    34981 gccagctgac gtacctttcc gctgcctgtt gccagctgtt gtgcacgcgg tatcttaagg
    35041 agaaacgttg tgcttgttga cataacacag tgtgctcacg cggggttaag gcgctgacgg
    35101 caccacccgt ttcagccagg acttagtgcg ccgggtaacg atgcggcccc cgcataacac
    35161 attgcgtaca gtgatattat agaagtttac tgtataaata aacagtaata tttggacaaa
    35221 acgcaaactg tgatcgatac tcaactatgc cctaaatacg tcaaaattcg tgccgaaatt
    35281 gcgcgttctg cgcggaacac gtatactttc agtgttgaca taatacagtg tgctttgcgg
    35341 ttaccagccg caggcggctg atgaaacctc gctccggcgg ggttttttgt tacctgcaat
    35401 tcagtacaaa acgtgatcag cccctcaatt ttcccttgct gaaaaatttt ccattgtctc
    35461 ccctgtaaag ctgtgcttgt ataaatattg ttaaacacaa aaccaacaag gtccccaatg
    35521 actacttcca tgttcaacgc aaaactacta ccaactgcgc tatccgccgc agtggtcgtc
    35581 gtgcgtgtgg tggtggtcgt cggcaatgcg ccgtagggac tggaacaaca cacgattcca
    35641 aaaccccgcc ggcgcaaacc gggcggggtt tttcgtttaa gcacctcccg gaaagtcggc
    35701 ccagaagaaa aggactggag catggcaagt tcgggcacaa catcgacgcg taagcgcttt
    35761 accggcgcag aatttatcgt tcatttcctg gaacagcagg gcattaagat tgtgacgggc
    35821 attccgggcg gttctatcct gcctgtttac gatgccttaa gccaaagcac gcaaatccgc
    35881 catattctgg cccgtcatga acagggcgcg ggatttatcg ctcaggggat ggcgcgcacc
    35941 gacggtaaac cggcggtctg tatggcctgt agcggaccgg gtgcgactaa cctggtgacc
    36001 gccattgccg atgcgcggct ggactccatc ccgctgattt gcatcaccgg tcaggttccc
    36061 gcctcgatga tcggcaccga cgtcttccag gaagtggaca cctacggcat ctctatcccc
    36121 atcaccaaac acaactatct ggtcagacat atcgaagaac tcccgcaggt catgagcgat
    36181 gccttccgca ttgcgcaatc aggccgccca ggcccggtgt ggatagacat tcctaaggat
    36241 gtgcaaacgg cagtttttga gattgaaaca cagcccgcta tggcagaaaa agccgccgcc
    36301 cccgccttta gcgaagaaag cattcgtgac gcagcggcga tgattaacgc tgccaaacgc
    36361 ccggtgcttt atctgggcgg cggtgtgatc aatgcgcccg cacgggtgcg tgaactggcg
    36421 gagaaagcgc aactgcctac caccatgact ttaatggcgc tgggcatgtt gccaaaagcg
    36481 catccgttgt cgctgggtat gctggggatg cacggcgtgc gcagcaccaa ctatattttg
    36541 caggaggcgg atttgttgat agtgctcggt gcgcgttttg atgaccgggc gattggcaaa
    36601 accgagcagt tctgtccgaa tgccaaaatt attcatgtcg atatcgatcg cgcagagctg
    36661 ggtaaaatca agcagccgca tgtggcgatt caggcggatg ttgatgacgt gctggcgcaa
    36721 ttgatcccgc aggtggaagc gcaaccgcgt gcagagtggc accagttggt agcggatttg
    36781 cagcgcgaat tcccgtgtcc aatcccgaaa gcgtgcgatc cattaagcca ttacggcctg
    36841 atcaacgccg ttgccgcctg tgtcgatgac aatgcgatta tcaccaccga tgtgggtcag
    36901 catcagatgt ggaccgcgca agcttatccg ctcaatcgcc cacgccagtg gctgacctcc
    36961 ggtgggctgg gcacgatggg ttttggcctg cctgcggcga ttggcgcggc gctggcgaac
    37021 ccggatcgca aagtgttgtg tttctccggc gacggcagcc tgatgatgaa tattcaggag
    37081 atggcgaccg ccagtgaaaa tcagctggat gtcaaaatca ttctgatgaa caacgaagcg
    37141 ctggggctgg tgcatcagca acagagtctg ttctacgagc aaggcgtttt tgccgccacc
    37201 tatccgggaa aaatcaactt tatgcagatt gccgccggat tcggcctcga aacctgtgat
    37261 ttgaataacg aagccgatcc gcaggctgca ttgcaggaaa tcatcaatcg ccctggcccg
    37321 gcgctgatcc atgtgcgcat tgatgccgaa gaaaaagttt acccgatggt gccgccaggt
    37381 gcggcgaata ctgaaatggt gggggaataa gccatgcaaa acacaactca tgacaacgtg
    37441 attctggagc tcaccgttcg caaccatccg ggcgtaatga cccacgtttg tggccttttt
    37501 gcccgccgcg cttttaacgt tgaaggcatt ctttgtctgc cgattcagga cagcgacaaa
    37561 agccatatct ggctactggt caatgacgac cagcgtctgg agcagatgat aagccaaatc
    37621 gataagctgg aagatgtggt gaaagtgcag cgtaatcagt ccgatccgac tatgtttaac
    37681 aagattgcgg tgttttttca gtaaccgctc aaggcttgaa caacatcgcg cttatcgtta
    37741 aggtaagcgc gttttttttt acccgccagg acaagaccat gatcaccgtt gcccttatag
    37801 acgatcacct catcgtccgc tccggctttg cgcagctgct ggggctggaa cctgatttgc
    37861 aggtagttgc cgagtttggt tcggggcgcg aggcgctggc ggggctgccg gggcgcggtg
    37921 tgcaggtgtg tatttgcgat atctccatgc ccgatatctc cggtctggaa ctgctaagcc
    37981 agctgccgaa aggtatggcg acaataatgc tctctgttca tgacagtccg gcgctggttg
    38041 agcaggcgct taacgcgggg gcacgcggct ttctctccaa acgctgtagc cctgacgaac
    38101 tgattgcggc ggtgcatacg gttgccacgg gcggctgtta tctgacgccg gatattgcca
    38161 ttaaactggc atccggtcgt caggacccgc taaccaaacg cgaacggcag gtggcggaaa
    38221 aactggcgca aggaatggcg gtgaaagaga ttgctgccga actgggcttg tcgccgaaaa
    38281 cggtgcacgt ccatcgcgcc aacctgatgg aaaaactggg tgtcagtaac gacgtagagc
    38341 tggcgcgccg catgtttgat ggctggtgat gaagacgttg ttctcccgct taattaccgt
    38401 tattgcctgc ttttttatct tctctgccgc atggttttgc ctgtggagta tcagcctgca
    38461 cctggttgag cgccctgata tggcggtgct gttatttccg tttggtctgc gtctggggct
    38521 aatgctgcaa tgcccgcgcg gatactggcc ggtgctgctg ggcgcggagt ggctgctgat
    38581 ttactggcta acgcaggcgg tcggtttaac ccattttccg ttattgatga tcggtagttt
    38641 actgacgtta ctgcccgtgg cgctgatctc gcgctatcgc catcagcgtg actggcgcac
    38701 cttgctgtta cagggtgcgg cgttaacggc ggcggcgttg ttgcagtcgc tgccctggct
    38761 ttggcacggc aaagagtcgt ggaatgcgct gttgctgact ttaactggcg gcctgacgct
    38821 ggccccgata tgtctggtgt tctggcacta tctcgccaat aacacctggc tgccgctcgg
    38881 cccgtcactg gtttctcagc cgatcaactg gcgcgggcga catctggtct ggtacttgct
    38941 gctgtttgtt atcagtctct ggctccagtt gggattgccg gacgaactgt cgcgctttac
    39001 gccattttgt ctggcgctgc cgattatcgc gctggcctgg cactatggtt ggcaaggggc
    39061 gctgattgcg acgttgatga acgccatcgc gctgatcgcc agtcaaacct ggcgcgatca
    39121 tccggtggat ttattgctct cgctgctggt gcaaagtctg acagggttgt tgcttggcgc
    39181 tggcatccag cggttgcgtg aacttaacca gtcgctgcaa aaggaactgg cgcgcaatca
    39241 gcatctggct gaacggttgc tggaaaccga agagagcgtg cgccgtgatg tggcgcgtga
    39301 gctgcatgat gatatcggtc agaccatcac agccattcgt actcaggcgg gcattgttca
    39361 gcggctggcg gcagataacg ccagcgtgaa gcagagcggg cagctcatcg aacaactatc
    39421 gctgggcgtt tacgacgcgg tgcgccgttt gttgggtcgg ttacgtccgc gccagttgga
    39481 tgatctcacc ctggagcagg ccatccgctc actgatgcgg gaaatggagc tggaagggcg
    39541 cggtattgtc agccatctcg aatggcgaat cgatgaatca gcgttaagcg aaaaccagcg
    39601 cgtgacgctg tttcgtgtct gccaggaagg gctgaacaac attgtgaaac atgctgatgc
    39661 cagcgcggtc accctgcaag gctggcagca ggatgaacgg ttgatgctgg ttattgaaga
    39721 cgatggcagc ggtttgccgc cgggttccgg gcaacaaggt tttggcctca ccggaatgcg
    39781 cgagcgcgta acggcgctgg gtggcacatt acacatttcc tgtctgcacg gcacgcgtgt
    39841 cagcgtttct ctacctcaac gctatgtcta aggtttgatg atgttgccgt ttctgaaagc
    39901 gcctgccgat gcgccattaa tgactgataa atatgaaatt gatgcccgct atcgctactg
    39961 gcgtcggcat attctgctga ccatctggct gggttacgcg ctgttttact tcacgcggaa
    40021 aagttttaac gccgcggtac cagaaatcct tgctaacggc gtgctcagcc gtagcgatat
    40081 cggcctgtta gcgaccctgt tttacattac ttacggcgtg tcgaagtttg tctccggcat
    40141 tgtcagcgat cgctcaaatg cccgttattt tatggggata gggcttatcg ccacgggcat
    40201 catgaacatt ctgtttggct tctcgacgtc gctatgggcg tttgccgtgc tctgggtgct
    40261 gaacgccttt ttccagggct ggggttcacc ggtgtgtgcg cgtctgttaa cggcctggta
    40321 ttcacgtacc gagcgcggcg gttggtgggc attatggaac acggcgcata acgtcggcgg
    40381 cgcactcatt cccattgtga tggcagcgag cgcgctgcat tacggctggc gtgccgggat
    40441 gatgattgct ggttgtatgg cgatagtcgt ggggattttt ctctgctggc ggctacgcga
    40501 tcgcccgcag gcgttaggtt taccggcggt cggtgaatgg cggcacgacg cgctggaaat
    40561 tgctcaacaa caagaagggg cagggttgac gcgtaaagag atcctgacca aatatgtgtt
    40621 gctgaatccg tatatctggc tgctttcgtt ttgctatgtg ctggtctatg tggtccgggc
    40681 ggcaatcaac gactggggca atttgtatat gtccgagacg ctgggcgtcg atctggtcac
    40741 ggcgaatacg gcagtgacga tgtttgaact gggcggattt atcggtgcgc tggtagccgg
    40801 ttggggctcg gacaaattgt ttaacggcaa ccgtgggccg atgaatttga ttttcgccgc
    40861 cggaattttg ctttcagtcg gctcgctgtg gctgatgcca tttgccagct acgtgatgca
    40921 ggcaacctgc ttcttcacca ttggtttttt tgtctttggc ccgcagatgt taatcggcat
    40981 ggcggcggca gagtgttccc acaaagaggc tgcaggggcg gcgacggggt ttgtcggctt
    41041 gtttgcttat ctgggggcgt cgcttgctgg ttggccgctg gcgaaagtac tcgatacctg
    41101 gcactggagc ggattttttg tggttatctc tatcgccgcc ggggtttccg cactgctgtt
    41161 actgcccttt ttgaacgccc aggcaccgcg cgaagcgtga tgcatctcac cttttcactt
    41221 catatccggc aaaactaaga aattttccag gttttgcctg gacgctatct caggcctgat
    41281 ttgctgctga tttttacaat gccatcattc attaattctt tcccaaaata attcaagttg
    41341 caggaacgca gcaaacgcgt gagtcccggg agcatagtga actatgtgac cggaaggaat
    41401 gcgtgcagct aaggcacctg cggcttgaag tatgaaggga aatcaggagt aacccatgct
    41461 ggctttctta aaccaggttc gcaagccgac cctggacctt ccgctcgaag tgcggcgcaa
    41521 aatgtggttc aaaccgttca tgcaatccta cctggtggtc tttatcggct acctgacgat
    41581 gtacctgatt cgcaagaact ttaacatcgc gcagaacgac atgatctcga cctacgggtt
    41641 gagcatgacg cagctgggga tgatcggcct gggtttctcc atcacttatg gcgtgggtaa
    41701 aacgctggtt tcctactacg ccgacggcaa aaacaccaaa caattcctgc cgttcatgct
    41761 gatcctgtct gccatttgta tgctgggctt cagtgccagt atgggcagcg gctcggttag
    41821 cctgttcctg atgattgcct tctacgcctt aagcggcttt ttccagagta ccggcggttc
    41881 gtgcagttac tccaccatca ccaaatggac gccgcgtcgt aaacgcggga cattcctcgg
    41941 tttctggaat atttctcaca accttggcgg tgcaggcgca gcaggtgtgg cgctgttcgg
    42001 ggcaaattac ctgttcgatg gccatgtcat cggcatgttt atcttcccgt cgattatcgc
    42061 gctgattgtc ggttttatcg gcctgcgtta cggcagcgac tccccggaat cttatggcct
    42121 cggcaaagct gaagaactgt tcggcgagga gatcagcgaa gaggacaaag agacagaatc
    42181 taccgatatg accaagtggc agatctttgt tgagtatgtg ctgaaaaaca aagtgatctg
    42241 gctgctgtgc ttcgccaaca ttttcctcta tgtggtacgt attggtatcg accagtggtc
    42301 aaccgtatac gcgttccagg aactgaaact ctctaaagcg gtggcgattc agggctttac
    42361 gctgtttgaa gctggtgcac tggtcggtac gctgctgtgg ggctggctct ctgacctggc
    42421 gaacggtcgc cgtggcctgg tggcctgcat cgcgctggcg ctgattatcg ccacgctcgg
    42481 tgtgtatcaa catgccagca acgaatatat ctatctggct tctctctttg cgttgggttt
    42541 cctggtcttt ggcccgcaat tgttgattgg tgtggctgct gttggctttg tacctaaaaa
    42601 agcgattggc gctgccgatg gtattaaagg cacctttgct tacctgattg gtgacagctt
    42661 tgccaagtta ggtctgggaa tgattgccga tgggacgccg gtattcggcc ttaccggctg
    42721 ggcaggcacc ttcgccgcac ttgatatcgc cgcgattggt tgtatctgcc tgatggcgat
    42781 agtggcggtg atggaagaac gcaaaatccg ccgcgagaaa aaaattcagc agttgacagt
    42841 ggcataaacg taactggtga cttttgcccg gcatgacgcc gggctttttt attattccgt
    42901 gacttccagc gtagtgaagg caaacttctc gccatcaaat agcccctgac tggttagttt
    42961 tagcgcgggg atcactggca gagaaagaaa cgccatctga ataaacggct catcgggtaa
    43021 cggaccgcat tcacgggcgg cggctttcaa ggcgtcaatt tgttccgcca gcgactgcgc
    43081 cgtgtcggtg ctcatcaggc cggcaatggg caacggcaga tgactttgga cctggccgtt
    43141 acgtaccacg cacagcccgc cgccatcctg aatcacctga ttgaccgcca gcgccatctc
    43201 ttcggcactg cgaccgatca ccacaatatt atggctgtca tggctgaccg tcgcagccag
    43261 cgcaccttca ttcagtccaa agccgccaag caaaccacaa gccggagcca gccgttgccc
    43321 gtaacgctca agtacggcaa taaagctcac atcatcgcga tcaaaaccat tttcgctgta
    43381 gacgctggag tgggagtgcg taatcaattc gttatggatg acgtcaatga cccgatagcg
    43441 ttttccgggc gtaaattgca gggcaaagtc gctggcggaa actggctggc gggcaatggt
    43501 gttgccatat ggcggagcgg attgtgccag tctcgccgac tcttccgcct gtaaggtttg
    43561 cgcatcaatc ggctcgcctt tcaccagtac ctgctgcacc gtgaccttac gcgcatcgct
    43621 caacaggacg atatcggcct gcttgccggg tgccagtaag ccgaggtgat tcagaccaaa
    43681 gtggcgcgcc gtcgaccagc tggcgacgcg atatgccaca tgcagcggca cattgtgttg
    43741 ttcgatcagg cggcgaatta aggcatcgat gtgtccttca tgggcgatct cccacgggtt
    43801 acggtcatcg gtacagagca tgcattgcgg gctgttaaat tcgttgatca acggtgccag
    43861 cgcgttgaga ttgcgggcag cggacccttc gcggatcatc aacgacatgc cgagttgtaa
    43921 tttccggcgt ccttcttcca gctgataact ttcgtggcag ttttcaatac ccgcagtaat
    43981 ataggcgtta agttctttac cacccaaacc cgggcagtga ccgtccagcg tcaggtggcg
    44041 aaatgcatcc agtttatcga gcagcgcatt ctgcccgcta attacgccag ggtagtccat
    44101 catttctgca aggccggtaa cctgcggatg gtcccgccag gcgagcatct gttcaagggt
    44161 aaaactggca ccgttaacat cgcagccttc cagggcgggt acgcaagagc tgacctgtaa
    44221 gtactggttt tgccttgcct gttcggcaca gcgggcaaac caggcgaatc cggcttctcc
    44281 catcacgttg acgatttcat gagggtcgca aataacggtc gtcaggccgc gcggcagggt
    44341 agcggtttca aaagtgaccg gcgtcatcat gctggattca atatgcaggt gagcatcaat
    44401 aaaccctggc accgccgttg cgccgcgagc atcaatccgc tgcaaagccg gagcatcagc
    44461 gtattctgcg ccaacaccgg caatgtaacg tcctttaatc acaattgggc cggaaatttc
    44521 tccgccattg atcaggtcga gaatagagac attatcaata atataatcgg caacagcgtc
    44581 gccacgggaa acggctaaca attcctggta ttcagcccgg ctaatgt
//
LOCUS       DAZFRJ010000015        43803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000015 DAZFRJ010000000
VERSION     DAZFRJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.015"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..547
                     /locus_tag="ACHKUN_000761"
     CDS             <1..547
                     /locus_tag="ACHKUN_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO6659072.1"
                     /translation="FVLFGDSTNLKNFWKYEVIRRKRMDIWRLLREKKQRNRNFLFWW
                     RLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHAGIVVHFAVD
                     AGENLVLRQNTTIGQIDGDMPGSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFI
                     NKDIPSNCTYITKKSGAVLYK"
     gene            complement(591..1169)
                     /locus_tag="ACHKUN_000762"
     CDS             complement(591..1169)
                     /locus_tag="ACHKUN_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6659073.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
     gene            complement(1169..2062)
                     /locus_tag="ACHKUN_000763"
     CDS             complement(1169..2062)
                     /locus_tag="ACHKUN_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6659074.1"
                     /translation="MSSKGRVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHLKGMT
                     AVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPTSGRAVLSQEQ
                     DGIKSHTHSASASSTDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGNHSHNIPVGHTG
                     AGNGVSAGFNAALGTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVN
                     ATGNEENTVKNIAFNYIVRLA"
     gene            complement(2543..3301)
                     /locus_tag="ACHKUN_000764"
                     /pseudo
     CDS             complement(2543..3301)
                     /locus_tag="ACHKUN_000764"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(3304..3834)
                     /locus_tag="ACHKUN_000765"
     CDS             complement(3304..3834)
                     /locus_tag="ACHKUN_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO6659075.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(3827..4723)
                     /locus_tag="ACHKUN_000766"
     CDS             complement(3827..4723)
                     /locus_tag="ACHKUN_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6659076.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTADAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(4727..5077)
                     /locus_tag="ACHKUN_000767"
     CDS             complement(4727..5077)
                     /locus_tag="ACHKUN_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO6659077.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(5074..5640)
                     /locus_tag="ACHKUN_000768"
     CDS             complement(5074..5640)
                     /locus_tag="ACHKUN_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6659078.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(5652..6287)
                     /locus_tag="ACHKUN_000769"
     CDS             complement(5652..6287)
                     /locus_tag="ACHKUN_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6659079.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(6280..6747)
                     /locus_tag="ACHKUN_000770"
     CDS             complement(6280..6747)
                     /locus_tag="ACHKUN_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6659080.1"
                     /translation="MLKTNSLRKGMIHGCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLTLRERVRITRSSQGAPQAEHLQEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(6771..8648)
                     /locus_tag="ACHKUN_000771"
     CDS             complement(6771..8648)
                     /locus_tag="ACHKUN_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6659081.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFAAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRSIIPDRMATAILNVAGR
                     TLAFISGKAPEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSE
                     GVFKITKQSNKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVA
                     LPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFT
                     ASSATVTPKLNGVAGPAFQVIKDSLTLGLNALTLTDITKNATYGVEIESLVLEINSPA
                     A"
     gene            complement(8787..9194)
                     /locus_tag="ACHKUN_000772"
     CDS             complement(8787..9194)
                     /locus_tag="ACHKUN_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO6659082.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRADNQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHAGNASPLP"
     gene            complement(9191..9583)
                     /locus_tag="ACHKUN_000773"
     CDS             complement(9191..9583)
                     /locus_tag="ACHKUN_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO6659083.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(9580..9903)
                     /locus_tag="ACHKUN_000774"
     CDS             complement(9580..9903)
                     /locus_tag="ACHKUN_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6659084.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            complement(9906..10106)
                     /locus_tag="ACHKUN_000775"
     CDS             complement(9906..10106)
                     /locus_tag="ACHKUN_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO6659085.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(10106..10600)
                     /locus_tag="ACHKUN_000776"
     CDS             complement(10106..10600)
                     /locus_tag="ACHKUN_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO6659086.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLTGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            complement(10702..11502)
                     /gene="gpM"
                     /locus_tag="ACHKUN_000777"
     CDS             complement(10702..11502)
                     /gene="gpM"
                     /locus_tag="ACHKUN_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO6659087.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADCGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(11548..12600)
                     /locus_tag="ACHKUN_000778"
     CDS             complement(11548..12600)
                     /locus_tag="ACHKUN_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO6659088.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNISYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(12624..13460)
                     /locus_tag="ACHKUN_000779"
     CDS             complement(12624..13460)
                     /locus_tag="ACHKUN_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO6659089.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVIGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            13615..15366
                     /locus_tag="ACHKUN_000780"
     CDS             13615..15366
                     /locus_tag="ACHKUN_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO6659090.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATVTGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            15366..16412
                     /locus_tag="ACHKUN_000781"
     CDS             15366..16412
                     /locus_tag="ACHKUN_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6659091.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(16902..17492)
                     /locus_tag="ACHKUN_000782"
     CDS             complement(16902..17492)
                     /locus_tag="ACHKUN_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6659092.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKYAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNRGWFSPLTTDKNNT"
     gene            complement(17556..17867)
                     /locus_tag="ACHKUN_000783"
     CDS             complement(17556..17867)
                     /locus_tag="ACHKUN_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6659093.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(17872..18831)
                     /locus_tag="ACHKUN_000784"
     CDS             complement(17872..18831)
                     /locus_tag="ACHKUN_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO6659094.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            complement(18908..21748)
                     /locus_tag="ACHKUN_000785"
     CDS             complement(18908..21748)
                     /locus_tag="ACHKUN_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6659095.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     ALEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDHRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARSDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPDEIETIVSHVCDIAIQEDRHELGNDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RTVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEPEDNAHENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAVHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            complement(21745..22134)
                     /locus_tag="ACHKUN_000786"
     CDS             complement(21745..22134)
                     /locus_tag="ACHKUN_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO6659096.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQKWQRVTGYQP"
     gene            complement(22207..22437)
                     /locus_tag="ACHKUN_000787"
     CDS             complement(22207..22437)
                     /locus_tag="ACHKUN_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6659097.1"
                     /translation="MANRKQQRAYAARRHIQTEINRRLFRASRVAQIMHINMLHERSY
                     ALSNIYSASVFSYLADDLRELQQLFQQQNKLH"
     gene            complement(22760..23059)
                     /locus_tag="ACHKUN_000788"
     CDS             complement(22760..23059)
                     /locus_tag="ACHKUN_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659098.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLNEL
                     EHARTTAPAIRLTLHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            complement(23056..23322)
                     /locus_tag="ACHKUN_000789"
     CDS             complement(23056..23322)
                     /locus_tag="ACHKUN_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6659099.1"
                     /translation="MTILDYITTHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHANPMPFGCSNPLTHMFNQLLKEART"
     gene            complement(23319..23522)
                     /locus_tag="ACHKUN_000790"
     CDS             complement(23319..23522)
                     /locus_tag="ACHKUN_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659100.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     DTYFCATNVARVNLIRQVQEPRK"
     gene            complement(23609..23722)
                     /locus_tag="ACHKUN_000791"
     CDS             complement(23609..23722)
                     /locus_tag="ACHKUN_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659101.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIINSVKKIERFFTGE"
     gene            complement(23719..23961)
                     /locus_tag="ACHKUN_000792"
     CDS             complement(23719..23961)
                     /locus_tag="ACHKUN_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO6659102.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            complement(23973..24251)
                     /locus_tag="ACHKUN_000793"
     CDS             complement(23973..24251)
                     /locus_tag="ACHKUN_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659103.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHGIISEAEKARKINGDEPYIEIICMDSEAEIQKAD"
     gene            complement(24262..24600)
                     /locus_tag="ACHKUN_000794"
     CDS             complement(24262..24600)
                     /locus_tag="ACHKUN_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO6659104.1"
                     /translation="MLKQRRNFCTGTERQANRFATSASRSNIRYSLSETHATPDGHTV
                     KQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQTDL"
     gene            complement(24615..24893)
                     /locus_tag="ACHKUN_000795"
     CDS             complement(24615..24893)
                     /locus_tag="ACHKUN_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6659105.1"
                     /translation="MTKLLNTYEQADFERLAAFYPYRDEHGLPVLEESLKDYAKRTNQ
                     TVNAVKRQADRAALPINQEEKNSKRTVNLFAIFLKTIRNAEKYVQMTK"
     gene            24985..25296
                     /locus_tag="ACHKUN_000796"
     CDS             24985..25296
                     /locus_tag="ACHKUN_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6659106.1"
                     /translation="MSSGYEKKLKEIRKSEGLTQAEFADVTGINLGTIKNYESGKREV
                     GLSVVDRVINSKDFEKYTIWLMTGKTNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            25385..26338
                     /locus_tag="ACHKUN_000797"
     CDS             25385..26338
                     /locus_tag="ACHKUN_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6659107.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGDALYEAS"
     gene            26817..27254
                     /locus_tag="ACHKUN_000798"
     CDS             26817..27254
                     /locus_tag="ACHKUN_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFO6659108.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLKCDIANLKGELKSDTAHLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            27447..28427
                     /gene="btuC"
                     /locus_tag="ACHKUN_000799"
     CDS             27447..28427
                     /gene="btuC"
                     /locus_tag="ACHKUN_000799"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO6659109.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            28490..29041
                     /gene="btuE"
                     /locus_tag="ACHKUN_000800"
     CDS             28490..29041
                     /gene="btuE"
                     /locus_tag="ACHKUN_000800"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO6659110.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            29041..29790
                     /gene="btuD"
                     /locus_tag="ACHKUN_000801"
     CDS             29041..29790
                     /gene="btuD"
                     /locus_tag="ACHKUN_000801"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO6659111.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            29868..30332
                     /gene="nlpC"
                     /locus_tag="ACHKUN_000802"
     CDS             29868..30332
                     /gene="nlpC"
                     /locus_tag="ACHKUN_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6659112.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            30579..31292
                     /gene="ydiV"
                     /locus_tag="ACHKUN_000803"
     CDS             30579..31292
                     /gene="ydiV"
                     /locus_tag="ACHKUN_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO6659113.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            31355..32791
                     /gene="selO"
                     /locus_tag="ACHKUN_000804"
     CDS             31355..32791
                     /gene="selO"
                     /locus_tag="ACHKUN_000804"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO6659114.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(32795..32986)
                     /gene="hemP"
                     /locus_tag="ACHKUN_000805"
     CDS             complement(32795..32986)
                     /gene="hemP"
                     /locus_tag="ACHKUN_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO6659115.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(33118..34164)
                     /gene="aroH"
                     /locus_tag="ACHKUN_000806"
     CDS             complement(33118..34164)
                     /gene="aroH"
                     /locus_tag="ACHKUN_000806"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO6659116.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(34321..35154)
                     /gene="ppsR"
                     /locus_tag="ACHKUN_000807"
     CDS             complement(34321..35154)
                     /gene="ppsR"
                     /locus_tag="ACHKUN_000807"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO6659117.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            35487..37865
                     /gene="ppsA"
                     /locus_tag="ACHKUN_000808"
     CDS             35487..37865
                     /gene="ppsA"
                     /locus_tag="ACHKUN_000808"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO6659118.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(37922..39568)
                     /gene="fadK"
                     /locus_tag="ACHKUN_000809"
     CDS             complement(37922..39568)
                     /gene="fadK"
                     /locus_tag="ACHKUN_000809"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO6659119.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(39625..39918)
                     /gene="ydiT"
                     /locus_tag="ACHKUN_000810"
     CDS             complement(39625..39918)
                     /gene="ydiT"
                     /locus_tag="ACHKUN_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6659120.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(39915..41204)
                     /gene="ydiS"
                     /locus_tag="ACHKUN_000811"
     CDS             complement(39915..41204)
                     /gene="ydiS"
                     /locus_tag="ACHKUN_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6659121.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(41260..42198)
                     /gene="ydiR"
                     /locus_tag="ACHKUN_000812"
     CDS             complement(41260..42198)
                     /gene="ydiR"
                     /locus_tag="ACHKUN_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO6659122.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(42218..42982)
                     /gene="ydiQ"
                     /locus_tag="ACHKUN_000813"
     CDS             complement(42218..42982)
                     /gene="ydiQ"
                     /locus_tag="ACHKUN_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO6659123.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            43298..>43803
                     /locus_tag="ACHKUN_000814"
     CDS             43298..>43803
                     /locus_tag="ACHKUN_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO6659124.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         9833 a        11192 c        11615 g        11163 t
ORIGIN      
        1 ctttgtttta tttggggact ctactaattt aaaaaacttc tggaaatatg aagtaattcg
       61 gcggaaacgt atggatatct ggaggctttt aagagagaaa aaacagcgta accggaattt
      121 ccttttctgg tggcggttgg ctaacgaaat gtatattaat ggtaataaat tacataagaa
      181 agcagccaaa aagttaaaca gtaaaataat taacaaattt ggttgtgaaa ttggattggg
      241 cgcaaatatt ggaaaagggt taacaattcc ccatcatgct ggaattgttg ttcattttgc
      301 tgttgatgct ggtgaaaatc tggtgttacg acagaatact accattggac agatagatgg
      361 tgacatgcct ggttcaagag taaaaattgg tagcaacgtt gatatcggag ctaattgttg
      421 catcattgga ttatcccgta aaattgggga taatgtaaaa ataggtgcaa tgtcttttat
      481 aaataaagat ataccatcga actgcacata tataactaaa aagagcggtg ctgtattgta
      541 taaatagagt acataaagcc atcgatattt ctatcgatgg ctttttcttt ttattgtggg
      601 gcgactggcc actcaacatc cgctgctacc gctgtattaa cacgattcaa caatacccgg
      661 tatgtcttcc atgcagccag taacgatgcc tcttcctccg ttgcaatatc caaatctacg
      721 gcatcctgaa gtggcgcaat atgctcgctg gctacctgca tcaggctgtt ttttgtttct
      781 tccgcctccc ttatccgaaa cagtttttct gcttctgcat ctttcaccca ggatgtgccg
      841 ttccacttct gatactctcc atacggcgac aaccaggtaa cattttccgg taatggaccg
      901 agttcagaaa taaataacga gtcccctgat gccacgtcat aaaccgtttt accccgatgg
      961 tcttcaacga gatgccacga tgcctcatca ctgttgaaaa cggccacgaa gccagccgga
     1021 atatctggcg gggcaatatc ggtactgttt gcaggcagac ctgtatgagg tggaatgtat
     1081 gcatcacctt caccaataaa ctcattggtt ccggtcagta gattataaat ttttatggtc
     1141 cgtgagtgtt cactcattct gaatgtcatt atgcaagcct cacaatatag ttaaatgcga
     1201 tgttttttac ggtgttttct tcgttacccg tagcgttaac ggtgatggtg tgtccgtggg
     1261 gaccaataat gacagaatgg gcatgagcac caatgccgat agtgtggtta tgggcaccga
     1321 tatatacatt gtgagcatgc tcgcctgcgc tcgacgtggt accagtacct aacgcagcgt
     1381 taaaaccggc tgatacacca ttccccgcgc cagtgtgacc aacaggtata ttgtgtgaat
     1441 gatttccagc gctattcgta gtttttgtgc cgtaatcaaa tgaactggtc gttttagtgc
     1501 cgtaatcaaa cgacgatgtg gttttcgtcc ccaaatccgt actggatgcg ctggcgctgt
     1561 gggtatgcga tttaatgccg tcctgttcct gagacaatac ggcccgacca ctggtaggtt
     1621 tgcccttaat cgtccagcca cgcatatcag ggatcacgcc tgacggataa gccgctgcaa
     1681 gtttcgggta tgcagatttg tcaaaagtct gcccctgcat cagggcatag ccagacggaa
     1741 cggtatctga tggccacggg attggtgcac cgactggata aaactctgca ggaggatgag
     1801 ccgaggtgta aagctgcgcc cacggcgacc agtttgcgtc ggtcgtatcc cgtcgtgaac
     1861 gaataaatgc cggagcatga gcaccgcttg taccactcca gccgatgagt aactcacctt
     1921 cgccaacggc tgtcatccct ttcaggtgaa tgatatttcc atacgctgtt ggatatccgt
     1981 tgttatacac ctcgtataac tcaagacctg ttgccccctg cgtattgtct gtcagcgcgg
     2041 ttacccgacc ttttgaagac agattaactg atgatactgc tgttccacct gacggtaacg
     2101 ccccgatctc tgatgccgtt ggcttatttc tggagttata gtcccttcgc cagccaggag
     2161 cataagcatc accatgatta atataagtaa attgagcatt agggatgcct tcaccgctgg
     2221 atgtactcgg tgtggtaatg cgtatggtca ttgcgccgcg ggtgccaata acttccacaa
     2281 cagcacctgc aagacaaata tttccgcaac ctgtgtctgt aatgacctta ttacttgcat
     2341 aagcccatga gcctttgcac atccagtaag gatggttaaa tgccccctga ctctctagcc
     2401 aaaaaatcaa ctgcgcagtt gtccatgctt cactatctcc accaatattc agctctgcac
     2461 tatatgcacg gcaggcaccg atatttttcg taaaggtgtc tttatcagag atatcctcac
     2521 cattcttagt tttctgcaga cgtttttcag cattgtcata ggcagctttt actgcatttg
     2581 atgttgccgc cagcgtttca gatgtactgt tggttgcact actaagctgg acaaggcctt
     2641 ttcgcgctgt tgtggcatcc tgcgctgtat attttccgtt agcaagatca tacgcgacct
     2701 taaccgcttt cggcgttgca gccagcgttt cagaatcgct gttagtggcg ctactgagtt
     2761 gaacaaagcc ttttgcggtc agcgaggcat ccgggtgacg tcgtgactgt tcatgctctt
     2821 tcagtttgtt atccacgtaa tccactgtgg ccatcaccat ggtgttatcc acggtaagcg
     2881 ccacagtggc agtgctggat acggtcagaa tggtgcgaaa tgtttgtgca cgtccggacc
     2941 cttcggcaac ggctggcttg taactttcgg cagtattgcc caccgcgatt aaatcgccgt
     3001 gctcatcaaa cacaccaatt tcccggatcc agaatccgcc cgtttctgga ggaataacca
     3061 gctccgcaat aatgcggttc tgatgtgttg cgtccaggat gacgcgatta acagtgtgtc
     3121 gccacacctc atgcaccaga cgggtctgct tactgtctgg tgtgggcaac gcgccgccac
     3181 cgtcgcccac ggccatatga gtcaggcgga caggcttacc atctggcgcg gctgcctgag
     3241 ctaatttttg ggcacctgta tcggtgataa cggttttaaa ttttcgtgtt gtggtactca
     3301 tgcttaatcg tccggataaa tggtaataac ttcaccgtca taagttgccg ccgctgcaaa
     3361 aatatccccc ggaatttcct gaatgatatt cagccctgtc atgtggcggc tgaccggacg
     3421 ggcatcagca atcaaccgct ccatttccag atacatttcc tccgtcacgc cactgtccag
     3481 tgtgccgact tcaacggtaa atgttcccgg ttctccgccg aactcccacc actcagacac
     3541 gcgaatgagg tatcccagcg gctcaatggc cctgcgcagt gcgctgatgg tccctttgtg
     3601 tcggtgtatc agccatgcat cacgaatcac ctgtcgtttt gtctcttccg gccagttgcg
     3661 atcccagcgg tcaacggaaa atgcccaggc gagataaggc agcaggtgca ccgggcaggt
     3721 gtccggcgac cacagcgtgt tgaggtctac cgggatgtct gtaatgcgcg ttccgacagc
     3781 ttcggcacaa cgcatgaaac tgctggctga tggtggtaac agtgaattac tcattgcgcc
     3841 caccttcgct gatggtgaac gactcacagc gcgccgcctg tatgtcgctg atggccatat
     3901 tctgtgtggg ttcgattatc tccacgcgtt gcacaccgtg cacatgcagt gcggcagcaa
     3961 tggcggacaa cgccacgtcc tgaccgataa gcccctgctc agccagccac ttcctgaacg
     4021 acgattcagc cgccgccaga ataggttcgg attccgggcc ggggtaaaag tacagttttg
     4081 cattcagccg ccatgtcacg attctggcgc tctgtacagt aaggcggtcg gccaccgggc
     4141 gggtatcctc tgcattcaga acggcgcgaa cggtattaag caacgcctcc gttgctgtgc
     4201 cgtcgccttc agtggacagg atggaaaccg tcacatttgc cggagacgga ctgatagccc
     4261 gcgcatcacg caccagaccg ctggcgctgc gggcaaaata ctcgtatgca cctgacgggc
     4321 cagcaacact caggccatcg tacgcccgct gcgcccgcag tctcagcgag gtgtcacttt
     4381 ccatcactgc gtcggcggta tccgttgccg gagtgatggt caggcgcttt gtgttcatat
     4441 tgcccgcgag gttgtccagg tctgtcccgg cgctgtggct taacatgcag gcgcgtgccc
     4501 cctcattgac ccgctggcgt aacagcattt cacgaaacgc tgttgtctgg gcgataacgt
     4561 tcaggggttc cgattccagc tccagcgcgg cggagacggc ttcacgctgt tcggcaggat
     4621 aggacgcaat catcatggcc tttgtgtcag ccagaattgc ctcaaagtca ggctccgcga
     4681 tgatggcggg ttccggtaac tgggaaaggt caacggcagg catgatttac tccctcagcg
     4741 tgatggttaa ttcaacattc tgcatggtct gcatgacagt gcccgacagc gtcaccccgg
     4801 cgcggcctcc tgccttccag acaacgtcga tggcgtccag ggcaatgcgg ggttcccatc
     4861 gtgtcagcgc aatcacggca gcactcatgc attgcagacg agtggtgtta ttcatgggtt
     4921 cgtcaattaa atcgggcaca aggctgccat attcccgtcg cataacccgg cttgccagcg
     4981 gggtggtcag gatgtccctg actgactgtt tcagatgctc catatcgttc aggtttcccg
     5041 ttccgtccgg gttcattcct gtgtagcggg ttgtcactgc gggcctcctg tcgaatcgct
     5101 gccaccttta acgccaccgt gtttatgcgt atgcactgtg atgccgtttg aggtgaaatt
     5161 gccgccgctg tgcgtgatat tgccgctcat ctttccccct tttgtgacgt caagcgtcgc
     5221 cgttctcaga aggtctgtgc attccacgac gggcgtgtcc agtgtcacac tgacggatgc
     5281 ctgcagggtg gccgttttca tgccgctggc gctcagtgcg cctgcgtccg cgtcgtagcg
     5341 gaacaccgcg ccatccggcg cgctgaccac gatttctttc aggcttttgc cgggggccgg
     5401 attggcatca ctccacaggc tgccaattat catggcggtt tccgggttgc cgccaatgca
     5461 ggcaattacc acctgttcgc cgggtgatgg cggcagccac acattgaagg ctcccgcgcg
     5521 cgtggtgttc cagcgcaacc agcctgtttc cagttcgccg ctgcgaacgc gcacgcacca
     5581 ggacttctca tcaacttcag agatgatccc agtgcggatg atgttgctca gcagtcgcat
     5641 gagttctgcg ctcaccgtac agcctccgca atccggccca gcaccgtgtt ataaatcagg
     5701 cgttcatctg cctgactgat gcccaacagc tcacgtaccg ggtaatcggt gaaaatgccc
     5761 ggcgcaacct gatcgcgctc accgaactga tgaacgcggg caatacgtgc ggccacgccg
     5821 ctgtaaccca ccgtcacacc ggaagcatct gcacgggctt tcaggtagcg ggcggtgcgc
     5881 agttttacga acatggggac gcgctttgtg ctgtcctggt tgatgcgccg ggtgcgtatt
     5941 tccagaaagc ggtcgatgtc atcccggtaa aacgtgcgga tattgttttt atcctcatcc
     6001 cacccggtaa tggttcgccc gtatttcccc gtgtcgtgat gccagttttt cagcgtgcgt
     6061 gcttcgttat tccagataaa gcgaatgcgc tcctgtatcc gggttacgcg gcgtctgcgt
     6121 ggtgtccacg cggtcccgtc cggcgctttc tgtgaccgga tacgcgcctg ctgggcgcgg
     6181 cgtaaatcct gtgccagctt tctggcgatg ttattgatgg cctgctgatt caggctgtcg
     6241 cggatagcct caaaggtttc atccacgcgg gtgaatgcct tatccatcgc tttcacccca
     6301 cgtcacatcc tggaatacat gcgaccagtc gccttcggaa gatggcagac ggggttttgg
     6361 ctcctgcagg tgttctgcct gcggtgcccc ctgactgctg cgcgtgatgc gaacgcgttc
     6421 ccgcagagtg agcgtaaaca ggagatcggc gctgtcatcg tcattgataa cggcggagaa
     6481 tttgatgtcc tgattacgct ccggattaag caacaattgc ggctgatttt cggataacca
     6541 cgccagtagc ggcagcgtga ggtcgtccag ctccccggcg taatccatga caaacatcac
     6601 catctgatag cggtaaacaa acgagggcgt ttctccggtc gtttcaatgt tgccgctctc
     6661 cacgaaaatg gtgaattttt ccgggttggc ctgacaccag cgacacccat gaatcatgcc
     6721 cttccggagg ctgtttgtct taagcatctg ttttgctctc ctgttcttcc ttacgctgca
     6781 ggactattta tttccagcac cagactttct atctcaacgc catacgttgc atttttggta
     6841 atatccgtca gcgtcagtgc attcagcccc agtgtcagac tgtcttttat gacctggaat
     6901 gccgggccag ccactccatt cagtttcgga gtaaccgtgg cactgctggc ggtgaacacc
     6961 agctccagcg tctgccagtc gttactgtaa ttcccgaact cgcccaactt tgtgtttcct
     7021 gctttcttgt gatgcatcag attcagtttg ccgtctgtgg tctgggtgaa gaacgacatc
     7081 aggaacgggt taccagtccc ggtcatcgcc acgacgtcag gtaacgctac atcggtatac
     7141 agataaattc ccagaccgaa ctgattgttg gtcagtgcgc ctgacagtcg aaacttacag
     7201 ctcagtctgc caccccgtgt cagcagggag actgcgtcat ccaccgggcg cgtcagggac
     7261 caggctttat tgctctgctt ggtgatctta aatacaccct ctgacaactg aattccgcca
     7321 ttcttaatgc tccagccctg cgcagcagcg tctccggctg tcggcaacag ggagattgtg
     7381 cgtacggatg catcttcaga cggccccgat ggcgtgtcgc cgccgggcga gggtttgatt
     7441 tccggtgcct taccactgat gaaggctaag gtgcgaccgg ctacgttcag aatagcggtt
     7501 gccatacgat ccggaataat gctcctgcgc gcccatgaac tgaaatgcgt ctggcgattt
     7561 gatgataccc agtttttgtt cgttcgggat gccgaaccgt aatatccctc acttgcagca
     7621 tccggatctt ccgttggtgc gttggtggag gtattgttgc cactaccatc ggtcaggaat
     7681 ggcacaaaga aaacgttgtc gctctccctg tttttgtacg ccccgtaaat ggtgtcgtac
     7741 tgcgtgccgt aggtgttttt ccagtaatac gtcgtgtcac cacaaatcca tggcacactt
     7801 gccgctctgc ctccatgaca ctgcgtgtta aactcggtaa tgtccgcacg aaactgtttc
     7861 agcatggccg caaacagcgg aggttgctgt gagtacgtag cggcgctcat gtcaaattcg
     7921 ccctgcatcc agcatacggc cagcagcatg ttttttgggt ttttctgcaa tgcggccttc
     7981 gtgcgcaaaa tcaggtcctg atataaaggt ttacccacac cccagcgagc cgaatcctga
     8041 ctggctcctg tggactcgct gaatgtcccc tccgcgccct gggtgaatgc cgaaccacca
     8101 cgacagcatg gaaccagtaa aatcccggca ttctgtggaa tgtaaggcaa caggcgtttg
     8161 gcaatatgca agccctgtcc gacacagcca tactgccctt tgctcaggtc tgcattcggg
     8221 tgattcagtg cgctcatatc ctgaacatca tgcaggcagt gatctgccgg aatgatgtcg
     8281 ttaaatgcgc atgctttacc accgggagtc actgtgttgc gacgggccag ttgcttaatg
     8341 cgtgggtggg gcgcatcgta agaatccggc agcggcattc cctcaccata tgacatggca
     8401 ttggattgac cagcaagtac caccacgtaa taccactccg gcacgctgcc gtgctggggg
     8461 gaatcaggtg agatgtctgt agtgccatcg gccaccattg cctgcattag ataccacggg
     8521 gtgccgggtt cgtaggcaac ccgcgtctcc ccctgaagat gccacccttt cgcaagatgt
     8581 tcattcacct tttgagtcag ttcagcctgt gtcatggctg taatcagtgc aaagttctta
     8641 ctggtcattg tgttactccc gtctggtgtg tcagttcggc tattctctta tccagttcag
     8701 caatggcccg tttatccgcg ttacaggttt ccagtgcatc cagaaggcgg tcgccccata
     8761 ttccgaggtt tccccatgtg ggttggtcag ggaagggggg aggcgttacc ggcatggtca
     8821 gcgtctgcgg tataagccgg actgacggcg ctggccgtgg cgcgttctgc gtgcctgcgc
     8881 aacctgtcag taaaacgagc gtcaggcaaa gcgtgggcgc attcatcttt tgcaatatcg
     8941 ttgcgtagct gttcacgtct tacctctccg tcctgattgc gttgctgatt gtccgcgcgg
     9001 agttgagcca gcacctgctg catatcctgt accccggcgc tgatgatatt cagtgtgtcg
     9061 acggtacttt tcagggtgct ggcctgcgct tcgtttctgg cgttctcccg gcccagcgac
     9121 cacgacagac gcatggatgt tccccatgcg gcaatcagaa ggaaagcgac gccaagcgtg
     9181 ggccagagct tcatgccgga taggctccgt gtggtaactg aaaatgcggt ccgtctttca
     9241 gggtcttcca gtcgccgccc cattccaccg gaatattcag ttcccggctg gcctgtctga
     9301 atgctgctgc gattttttcg tacagcggcc attcccatga cacctggctg ccaacataag
     9361 ccacaacatc cacggcatgc cctgtgagat ggcgactgtt catggtctgg ctcttgcctg
     9421 tggtcacgag ctgcttctga cgataacgac tgcgcaatcc ttcggtgata ccaaaatcca
     9481 cttccgagat ttccagcgcc cgtcgggtca ctttcaccag atcaggattt acaccctgca
     9541 aattcttttc gcttcggctg ctgaatttaa atgtgttgct cattcgttcc tctccttcac
     9601 cctgcgatca aatattgcaa taaccttgtc gcgtgctttc tctgcgccca taaaaccgat
     9661 tgaggcacca aaaaacgtca ctgcatcctc agggatcccg aagaagcgca gcgacccggc
     9721 cacggccatg gcaagaacgc cgcacgccag cgatcccgtt acggtctgaa ccagtgttcg
     9781 tccgtcataa agactcatca gcgcggaaat gctgacagcc gcgcctactg catacaccgt
     9841 tggcaggtgg tcaaagagcc acgcaataac ctgctctgtg atccctgttt gaatggtgct
     9901 cactgctact ccccccacaa ctgaatcatt tctcgtttct tcttctccgg ctccggcatc
     9961 tccacttcct gcccggcgtc cagaaatacc tgctgacaga gtccggggtt ggcatccagc
    10021 accttttcgg tgacgccctg tgtcgtgccg tagtaccgga aacagagcga atccacggtg
    10081 tcgccttcca gtgccttcac tttcatcagc acagctccgc aaacattcgc ggtcgtccca
    10141 gaatgtcaga gatggcccag ctcacatcgc gccacaaatc cgatgtctgt atatccagtg
    10201 cgtccgcccg gcggtcgccc ttgtccgttg tgtccgcatc gcggtaacgc tccagaatca
    10261 gggcgcgtgt ggcggtataa acagcattgc gccagtgcca gagattgacg ctttctccgt
    10321 taattacagg ggccggaaca tcggccagcg tctgatagcc agccgcctgt tgttcctgct
    10381 gccatgcttc cagttcgcgg gtaacgtgtg ctatggcccc ggtggcggta tgcagcaggc
    10441 gggaggtggt cacgcggccc gtcagtcgta ccgccagacg cagctcacgc agcacaatat
    10501 ccggccagaa tgcacccgct gaaatgcgag tgtcgccatc atcggtatcg gtgatgtcgt
    10561 cctctgcggg gccggggtta gttctggcaa ccatactcat ggggttcact cctgaaaaaa
    10621 tcgggcggtg ggtgcgcggt gtaaacggtc acggagtcaa accggaacac cgcgcacgcc
    10681 gcccgctgac ggggtcagtc gttaaccgcg ctttgccttc tgcgtcgcgg tggtttttcg
    10741 tgttgcaggc ttccgcgttg tctttttact tttgctgctt tcgtcctgcg cctgctgtgc
    10801 gctggcgtct tctggttcgg ctgcggaatc ggcttttttc agggcgcggg aaagggttgc
    10861 aatctcgcgt ttcacacctg cgttcgggtt caggtgcatc gcttcgcgca gcagcttcag
    10921 tgatgaggcc atgttgtctg catcggtcag gccacgacgg gcaaaggcac acgccttgca
    10981 taatttggcg cgcacttcgt ccggcatgtc ctggttggcg acaatttccc aaagtgtgtc
    11041 cagtggttcg ataaaggcgg ataaatccgc gtcggcatcc gtcccggcct gcgtcagtac
    11101 cgggttgcag atttcttcgg tcagtaccgt ggcagcagta cggccaaagt tatccggcat
    11161 gatgaggttg tgacggacca catatgcacc aatacgcagc gccagcggaa gatcgccgca
    11221 gtcaatcgcc cacaccatca gcgtggcaat tacttcgtcc tgttgcccgc agtcagcctc
    11281 cagcgttccc tcaatccagc cggaaaagtc cggcaacaac tcttttttga tggcggcttt
    11341 cgcgcttctg gcctgtatgg cctttagcct ggcctgtgcc agacgcagac gatacagcac
    11401 ctcttcatgc gcggtacacg cggcgtggtc cacaccttca ttcgcccggc ctgcgcgctg
    11461 tgccatcacg ttctgccagt gttgttgtgc aggagtaatc attttttctc tccgttacag
    11521 gcgggcatga tgcccgccgt gagttgatta gctgtcggcg aacttcaggc cagtgaccat
    11581 cgcgcacttg ccatagtctt caacgacata agcgtcattg atggactggt aggtggcgat
    11641 gcggttgtat tccggctcgt ctttcatcag gcgacgcatt gaacctttct gccagtaaat
    11701 cgacaggttg ttgaacgagg tgataagcat cgttgcatcc gggaagaacg gcgcaaggaa
    11761 cacgcccagc ccgccaatgg tgcgcgatga caggatgagc tgcccggcaa gtaattccgc
    11821 attgggattc tggccgctga tgctgttcag cacgggcaga cgcagcgagt taaacaggtt
    11881 gcgccccata atcaccacga ggtcgtcagc ttccttgtgc cattcatcca gcagggatga
    11941 gcgcgcatcc tgtaccagag catcggcatt cgcatactta cccgcgtgcg ccacagtgtt
    12001 gtccatgttg cgggaggtca gcgtcacgtc attcattacg cgctcgctgg catcggttct
    12061 gatgtgctcc agccagccca cgttaacgtc ctgaagcagc ttgttggtgt gaaaatcaga
    12121 ctcgtaggcg tgggatgtgc cgttaaagcc aatcatgatg cgatcaagcg ctacctgccg
    12181 ggcgatctgt gtactgatac gggactgaaa atcgctgtgg gccgcccagg tatcaagctg
    12241 tgggtacgaa ataaaggtat cgtagttcac ctgttcgcac tggtactgac gagacttcat
    12301 atcgatcacg ttattcgggt tacggcgttt gatgccgtca taactgatat tcgtgcgcgc
    12361 aattggcccg gtggtgtcca ggaggatttt ttcgcctttc tggtcggtca cgccgatcac
    12421 gttaattctt ttcgtaaatt cggtactttc ctttgaggcg ttttcaaaac gctgctgcac
    12481 cgcgggttcc acggtaaatc gcgataccag tgcagatacc gggatattgt taagcgacgc
    12541 ctgctgcgcc atatagcaac ccagcttgtt gcgggtaata tctgacatca ccagattcat
    12601 aaaaaatttg ctcctttgtc ttatcagaag tcagccagct ggtcggaggc tgcgcccgtt
    12661 gcggtgaagc ggttctgcgg atcgccgtcc tgcgtgcgca gtttttcctt cagtgctgtc
    12721 agctctgtgg tcagtgacgt gattttctgg cggtcctgct gatggcgggt ttccagcaca
    12781 ttaaaacggt cgataatgtc ggcctgtgat gttgcaacgc cttccaccgc ttcctgaata
    12841 cgggagaaac tggcgtcatc cgctttgcgg ccacgaccaa taatccctat tacgcggtta
    12901 aaccactggg tgccttcttc ctggcgttgt tctgccattt cgatgatttc agactcgatg
    12961 gcttcggaga taagcggtgc ttcaccctgg atactgttga acgtcatcac cgcctgacgc
    13021 tgctgtgccg tgaatttcag gcgctcagtg cccaggcttg ccggggtgtc ggtcatcgcc
    13081 agcccgacca gataggcacg cccgttaacg gagaactgcg ggtgcagttc gatactggaa
    13141 tagattttct tgccgtcagc gacaagctgc ttcatgcgct cggtcggttc gatttctgca
    13201 tacagcgcag tgcgtccggc cagcgggcct tccgtaatat cttccgtact cagcgcggtg
    13261 acatcgccca ttgcggaaaa ttcgcttgac gggcatggcg agagatagtg ctcaacgttc
    13321 acgcgggcag cgtaaacatc cgggttgaag ttctcggcgg cttcacgcag atgcaccgga
    13381 ctgatttcac ggccatcaac agttgatccg gagacagcca cgcgaaactt tttgcgggat
    13441 gtcttttttt cattagccat agtttttgcc cctctgactg gttcttcagt catgatggca
    13501 aagcgtaaca ggctgataca aagggatttt gttgtaagaa aacggccaga acagggggtt
    13561 aaggagaaca gtttcgcgcg cgggtaatct tcctgtaatt actcaggggg agcaatgatt
    13621 caggacgctt ttgtgcgcca gcgtgcgcgg caactttact ggcagggtta tccgcccgca
    13681 gaaatatcac gtctgatggg aataaacccg aacacgattt atgcgtggaa aaaacgcgac
    13741 cagtgggatg aaacgccacc cgtgcagcgt gtcacgcagt ccatcgatgc gcgcctcatc
    13801 cagcttactg aaaaacagaa taaaacaggc ggtgacttta aggaaataga cctgctgacc
    13861 cggcagctta aaaaactgca tgatggccag ccggatgcga cggtcacagg aaagaaaggc
    13921 cgggcgaaaa aactcaaaaa tcatttcacg ccggaacaga ttgccgcact gcgggaaaaa
    13981 atcatcagca ggctggagtg gcatcagcgg ggctggtttg actccctgac cctttgcagg
    14041 gaagccggga tacgtaacag gatgatcctg aaatcccgac agattggggc gacctggtat
    14101 tttgcacagg aagctctgct gatggcgctg cgtgacgatg tggcgcaacc ttaccagcgt
    14161 aaccagattt ttttgtctgc gtcgcgtcgt caggcgttcc agtttaaaag cattattcag
    14221 aaggccgcgg ctgaagttga tgtggagctg aaagggggcg ataaaatcat cctctccaac
    14281 ggagcagagc tgcattttct cggcacttct gctgcgtcgg cacagtccta tacgggcaat
    14341 ttttattttg atgaattttt ctgggtcagt cgctttgctg aactgcgcaa ggtggctggc
    14401 gctatggcaa ccctcagcgg actgcggcgc acctacttct ctacgccatc caccgaaacg
    14461 cacgaggcat acgcctactg gaacggcgac cgctggaacg agaaaaaggc cacgcataaa
    14521 cgccagcgtt tttctgtgga ctggaaaacg ctgcataacg ggcttatctg ccctgaccgg
    14581 acgtggcggc aaattgtcac gctggaagat gtggtcaatc acggctggaa acacaccgat
    14641 atcgacgaaa ttcgtgatga aaacaccgaa gacgagttcc tcaatctcta tatgtgtgag
    14701 tttgtccgcg aaggggaatc ggcatttaac ctgaatatcc tgattggctg cggtgttgac
    14761 ggatacgacg actggaaaga ctggaaacct tttgctcccc gcccgatggg gaatcgtccg
    14821 gtatggattg ggtatgacgc aaacggcagt agtggcaacg gcgacagcgg cgctgtgtcc
    14881 gtggtggttc ctccggctgt tcctggtggc cgttttcgaa cggtggagac gcgacgcgtt
    14941 caggggctgg agtttgaaga acaggccaga gtcattgaag agttcacgta tcgctacaac
    15001 gtggaacaca tcggcattga tgtgacgggc gggaacgggg aggctgttta tcagatagtg
    15061 aaacggtttt tccctgctgc tattccgtac accttcacgc tgtcatcaaa acggtcgctg
    15121 gtactgaaaa tgctgcaaat aatgcgtgcc gggcggtggg aatacgatcg cgccgaacgc
    15181 gagctggtcg cggcctttaa cgccgtgcgt aaggtgaaaa caccgggcgg ctttatcact
    15241 tacgaaacgg accgcgcgag ggggatcagc cacggcgacc ttgcgtgggc aaccatgctt
    15301 gctgtcatta acgaaccgat tggcggcgaa ggagaaaacg agcgtttcac ggttatggag
    15361 ttctgatgag cagaaaaaat aaaaaagtgc gcatgagttc acgcattgat ctcgctgatg
    15421 cgctcaggaa agaatcatcg ctcagtgcat tcacatttga tggtccttat cgcctgaccg
    15481 ggcatgacct gctggacaat atgtactgtg ctgataacgg acggtggtat gaaaccccgg
    15541 tggactggta cggtctggca agagcagccc ggcaaacgtc ctggcatcag tctgcgcttt
    15601 actttaagcg caatgtatta ctcggttgct acatcccgca cccgctgctt tcccggcagg
    15661 atttctcggc gctggcgctg gactggtttg tgttcggtaa cgcattcctt gagcttagaa
    15721 gcaatatgct cggcgaaccg cttaaattac ggcacgccct ggcgaaatac atgcgacgcg
    15781 gaagcgatct tgaatcatgg tggtatgtgc aggatggcaa ggacgcgttc cagtttcgcc
    15841 ctggcaaagt gtgccacctg atgaatccgg atattaacca ggaaatctac ggcatgccgg
    15901 aatatcttgg cgcattactc tcggccagcc tttctcattc ggcggacatg ttcagaaaac
    15961 tgtactacga caacggatcc cacgccgggt gcatcatcta catcggtgca gcgcaggtaa
    16021 accgcgaaag catggactcc ctgaaagaaa cgttacaggg cgcgcgtggt ggtggtgcgt
    16081 ttaaaaacgt actcatccat gcgcccaacg ggggcaaaga aggtgtgcaa attttgccgt
    16141 tccagcagat cactgcaaag gatgagttca tgaatgttaa ggcggcatcc cgtgatgatg
    16201 tgctggctgc gcaccgcgtt ccgccgcaac tgatgggggc gatgccgggc gaaaaaagtg
    16261 cgtttggtga tgtggagaag gccgcgcggg tttacgcaat taacgagctg atgcccgtca
    16321 tggaggccat gaagcacatc aatgactggc ttggcgaaga ggtgatccgc tttaaccctt
    16381 acgcactgct agatacccag cccacatcct gacgcgcttc gcttgtctgc tgcttcgccg
    16441 gggcataaaa aatttatgcc ccgactctcc agctcctgta tcaatcagat aatttcacga
    16501 cgcttttctg cttattgtca tcatcggcgg tcagactctt acgcaatccc accgcgttga
    16561 ctgcatgttc tcgccgcctc agtgcgattt tgacggcctt atctgccacc catcaaatca
    16621 aaagccctca cgtctttttc acgctcagcg tgagaaatac agccattctg ttgtgtcgct
    16681 gcgacatcgt tcaggggatg ctatttaccc cctgaaacgc gggctgttcc cccgtcacct
    16741 gcgcgcagaa aaagcgcgtt tttttgtgca cgcacggatc cttgacggat ccagccgcca
    16801 tgcgggccgg aagggtaaaa agtcgttcaa aaaaattgtg caaacttgtg cactattgtg
    16861 cagcagggcg atgcgcgatt atcgccctgg tttgaggggg atcaggtgtt atttttatct
    16921 gtcgtcagtg ggctaaacca tccacgattt acatcgcagg actgatgcgt tctgtcctcg
    16981 tgtttctcct gccacaatgc ttttccgcca gcctttatga tggctctttc aacatcccgc
    17041 tcgtatcgca agggctgaaa tacgtcatta atgaaatatt cgcttatgtc cggaagcgat
    17101 actgcaaaat tatttgtttg ttgcgccgtc ctgctatgca gcaaagcggc atactttaat
    17161 gcgtcctgtg ctttctggaa tttaagtgca tagtcagtgg cgattttttc cagttcagcg
    17221 attcggttgt ctttggtttc cagccaatca agtagcgcca atacttcagg atccccgaca
    17281 tccaacactg ttacgcgtga tttttcataa tgttcgtcgg caagagttcg gccaattttg
    17341 aaatctccat catcaccata accggagcag gcataaacaa cctgtgctcc agatattcgc
    17401 tgaattgaca tttcctcgcc gcaaataggg catttcggta ctggcacagg tgaataacgt
    17461 tcacgtaatg cctgatagtt gatttcgctc attaagtcgt tctccttgtt tatgttctga
    17521 ggtggagagc agggcgaact cgccctgatg ttgtgttatt caggaaataa cgcccggata
    17581 tttccggcca tctgactggt tatctgtgcg gttgatactg gctgtgatgc ggggcgttct
    17641 gtcctggttt gtgtcactga taacgcttca tcaccagccc atgcagccag tcgataagcc
    17701 tctgccggat tcattttcag aagtgccagc ccggccagaa aagccacgcg ttggccgctt
    17761 ttgcgggctt ctggtgtaag gctgtccagc caggcgcatg cttctccttc gttcttgacg
    17821 gcggtgggct tcagatagaa acttatccgt ctggttggag tcgtcattgg tttactcctt
    17881 gtccattgcg tacagcccat taaccagagc aaactgtggc accccgtccg cgatgaaagt
    17941 cgcattaact ccgcaggctt cgcggatagc gggtgccaca atctccgccc ctccaccgac
    18001 aaccatcacc cgcccgtaac ccgaaaaacc cgccagcgcg cggatcacgc gttgtttcag
    18061 tgtttcttcc ttttcacgaa taaccgccat caggctggcg taatgcgcgt cattgtggat
    18121 gtgctggcgc agccaggctt catcatggcg atgttcgata atggtattgg cgatgtggtg
    18181 actggtgcgc ataccgttag tggccatcac cgacagtacg gcatcggcca tcagagaaac
    18241 gcccacgtgt ggatcgcaaa acacctggct gatacctgcc agttgcccct gaacctttgc
    18301 cacatccaga gtggttccgc ccaaatccac aatcagcagg gattcaaacg gactcatgtc
    18361 agccagtgcc ttaaagccag ccggaatgga ttcaggcata acccgtacgt tacggatagt
    18421 gaatgcttcg ccgttctggt actccaccgg gcgcatgacg ttcgcttttt tgcggttgat
    18481 gttggccatg tccggctgtg cgtttgtatc gaaatactcg ctcagtggca gggtgacaac
    18541 cacatccact tcctgtggtg tgatgcctga tttgaccagc gcgtgatgaa tggcaattat
    18601 attcacatcg ctgtactggt attgcgtgtc ggtcgtctgg acaaagcgat cgctgaccgg
    18661 atcaaaacca tagcgcacgc catcaagcat gtagttcgcg ggctgcgtgc caccgaacgg
    18721 cgcagaccat tccgacttga agctgttcgg gctgatggcg ttgcggcgtt cgccgttctc
    18781 agtccatgcc agcttgatgt tggtggagcc gtcgtcgata caaattttca tgtcgctttt
    18841 ccttgtgttg attaattaat cggttacagg atttttaaat cccgcttttg cctgttttgt
    18901 gcgcgcttca tatatcgcgg cacgtttttt gctcatttac gggatttgtg agtcccgttt
    18961 ctgtctgttt tttgtttcca ctggtcaggc taccccgcag caggtctgct ttgcggtggg
    19021 cgcgttcagt ggtttcactg attctctgtg cgtgcactgc gtcacggatg gcgcgcagca
    19081 tgtcagaaag cacggtaacg ggggttttca tggtgttctg gtcctgctga agtgtggatg
    19141 ccaggtgtgc ggcggcttcg gggtctgatg cccccagctg ttccagatag ctggcgaccg
    19201 ggttatggcg gatctccgtg ctgcttacgc cgtgattacg gctcaggcgc tgccagagtt
    19261 gcgtgattcg gctgtccggg cgggtatctg gtttgcgtac aatttcaaat ccctgcggtg
    19321 caatgatgct gccgtcaacg tacagactgc cgccccgtaa caggtgctgc atctgctgtt
    19381 caccgatatg caggccgaga gattcagcag actcccgcca ttctttagcg agtaactcgt
    19441 ggttatcagg caaaggccgt ggctgtttgc ggctctgtgt ccagttctgc atttcatcac
    19501 tgctgttttt tgcctgtttg tcacgcagcg aacgcatcag cgcccggcgt tcgtgccgtt
    19561 tcagtgagcg catccattca ttcacgtcaa cgccgtcagg aagctgcggc cacggtgctg
    19621 gcctttcttc cggctgttct gtcccgttgt tgtccgtttc ctgtacacgg ggacagttat
    19681 tgccacgagt ccaaggggcg gcagggccgc cctgaaggtc aaaaccattt tcgtgggcgt
    19741 tgtcttctgg ttctggttta cgtcttacca acttccagtt atccggatgc gtgcacacac
    19801 gggaggattc cccgatgagt ggtgaccaga tcccgtaaat ctgtacgctc tgttcgccgt
    19861 aatcgttcag ctcatctgcg aggtcgtagg cggtgcgaat caggtagtcc ttgcgtggaa
    19921 caagcacgcc accctgtttt tcaatgtagg tggcaaaaca cccggcatcg gcggcagcga
    19981 gtaccgcatc cattgcatca tctttcagcc gttgcgggcc ttccggattg cgtgccatct
    20041 ggctggcaag gcggcgcagt tcacgccaca cctgacggga ggggatgcca aagaactgga
    20101 actgacggac ccggtgaagg cgcgcccagc cgatggcgcg ctccacgctc tcggccattg
    20161 atttacctgt ttcgtggtca acgcgtggct tgcccgtttt cgggtcgatg ccatccacgg
    20221 tgcggctgtc caggttcttt ccgatgtagg tggcgatgta gctggttggc gtgccttttg
    20281 agccgtcgac gtactccgcc ttaaaacgcg gagttatgtc attgcccagc tcgtggcggt
    20341 cttcctgaat ggcaatatcg cagacgtggg acacgatggt ttcaatctcg tccggatgtg
    20401 caaagaccat catatgccag tgcacagtgc cgtcatggtg aggctccacc gtgcggatgc
    20461 cataccagcg caggccgtcg cggttcagtt ttttgcggac cgccgcaaaa aacgtgttaa
    20521 ccaggtaatc gctagagtcg cgcatggtgg ccccgttcca tttggggttc ggatgaccgt
    20581 tctccgttgt tgcgtggtat tttgacgggc aggtgacggt cagaaacacc gctctgtcgc
    20641 tacgggcttc ggccagaagt tccagtccct tcatggtggc catcatttct gccttacggt
    20701 gaaccgggtt acttactccc gcgtaataca ccgtctcgag atcaatcgtg aacccgtctt
    20761 cattttccag catgaaactt ttcaggaaat cgcgtgtttt ctcgcgctgt gcgcgaaact
    20821 cgcttaacgc gtcctggctc agatagggcg atgtttttct ggaaaccaga caggcggcgc
    20881 ggagttgttc ttcccgccac tcgcaacgta acagccacag tttgcgtttc caccattccg
    20941 cacaggtcag gcgaaggatt gcgcccggca gcagctccgt gtctggttcg ttcctccggt
    21001 ctttgtctgt tgtcagtgcg tcataatgtg gaggcatggc gtgcaggtgt aacgccatgc
    21061 gggccagcat ctgatacgcc ttcagcgtta catccatggt cagctcgcca tcggtcgcgc
    21121 caaagccatc gcagagtttt tcgaaggtgc tgctgaacat cgccgccgtc atggtggcca
    21181 gcgtctgtat ctggtgtttg ttgagctgcg gcaggtaaag caaatcgtcc aggcgttcgc
    21241 gtccggcaag ggagcgataa cccggtgtca gccagcggtg atcggtgcgg tccagacgtt
    21301 cgaatatttt gcgcagggtt ccgcgcgcgt agcgttccgc ctgccagctc tttttgcctt
    21361 tccggcgatc ggcttcctgt tttttgcgca ggaaagagag gtggcggctc agaggttcac
    21421 gcagataaac gggaagcacc ttcagtgtgg caaaagcacg ggccaccggg tcttgttctg
    21481 ttgcctgacg cttgctgatg atgctttgtg ccagcttttc acgctgtccg gcttcctcaa
    21541 gggatgccat gagtttttta cccacggtgg attgtgcgaa aaaggcttcc tccttcgctt
    21601 cctgttcttc cagagccttt ttgtctgcct caaggtagta acggatggcg cgttgcaggt
    21661 cggtttcagt ttcctgcctg tgctccgtaa atctggccgg atcaatggct ggccgtggtt
    21721 cattccagct ccatgcaaac tcactcatgg ctggtatccc gtcacgcgct gccacttctg
    21781 cgagaagagg gcagaaaggc ggttaaattc agcggtgtat tcactcagcg aggcacaccc
    21841 gccagcagtg cgatgcgcca gcattgccgc aaatacggag gccggggagt cgtaatacgc
    21901 cagcagtgat tctccgtgtg gtgtcaggca gtgcaacgcc agcccgtgtg gtgtgaagtc
    21961 cacgcggtag cagtcgtcta ctgtgaaata aagggtgtct gcattttccg gttttgtggt
    22021 gcgtgctctg ttgtcacgac cacggatgta gagatcaaat aatccctgaa gaacgggagc
    22081 cagacgggtg tcctgtgtgc gcacccatct tgtgaagtca tgagcgtcaa tcatgctgca
    22141 attctcttta ctacagatgt gcgaaggcct cccgccgcaa ggtgcaggaa aggcccggaa
    22201 caggaattaa tggagtttgt tttgctgctg gaagagttgt tgcagctcgc gcagatcatc
    22261 cgccagatag ctgaaaacag aggcggaata gatgtttgat agtgcgtagc tgcgctcatg
    22321 cagcatattg atgtgcatga tttgcgcgac gcgtgatgcg cggaaaagtc tgcggttgat
    22381 ttcagtctgg atgtgacgac gcgcagcgta tgcgcgctgt tgtttgcggt ttgccatggt
    22441 gtggcctctg tagttgcaag ttttgaaaac tcaccatcca gaggtggaaa actcggggtg
    22501 gtgagacgta cagggtttcc acaaccggca actacagaac ccggcccgac cgaagtcggc
    22561 cccgtacgcc ccaccataat tcgtgtgcga aaaagacgtg gcgatacagt acgcacaaaa
    22621 aaaccgctgg cgcggttgtg cgctgtagtt gtcagcgggg tggaaatccc ggcacccgtt
    22681 ttatgaggtg cagcggaaat gtaacctgac tgattgcggc atggcaagcg gtttttttgt
    22741 gtgtgcatgt tctggtttct tactggttca gaaaaaaatc aaaaaccttg tcaatgcgtt
    22801 gcagcagctc ttgctgtatt gcttccggcg tttccggttc gcccggcgcc tccaacgtcg
    22861 cgcagaaatc agcgatttca tgatggagcg tcaggcgaat ggcaggggcc gtggttctgg
    22921 cgtgctccag ctcattcagc agtgccagca cagcagacgg cgagagcatt gcgcgaaatg
    22981 ccagtaattt ttgaggcgtt gccattcgtt gcagggcaaa tgccagttcg cgtagcttct
    23041 ggtggttgat ggtgctcatg ttctggcttc cttcagtagc tggttaaaca tgtgagtaag
    23101 tggattgcta cacccgaacg gcatcgggtt tgcgtggtaa gaagcctggc ctccagtttt
    23161 gcgagcgcga ccacctgtgc tgcggtttgt tctgatgact aagccgccgc gccaaagtcg
    23221 gcgtaactca gcattaatag ctgtggttgg ggtgttcagt gctgcggcga tttctccgcc
    23281 gctacaaccc ggatgtgtag tgatgtagtc cagaatggtc atttgcgtgg ctcctgtacc
    23341 tgtcggataa gattcacccg cgccacgttg gtggcgcaga agtaagtgtc gtcagtgagg
    23401 tagatgtggt gtgcatcctt ttccgaacgg tgtttgtcga ttgtggtaat caggcgttcg
    23461 tcgacttcgt attcacgtcc tctggaggta aaacgaacga caggaaaatg cttaattgcc
    23521 attacgcctc cttggcgtgt gtgaatacct ccgcgaatgc ggattgtttt tacattttct
    23581 tatttaacct gtggttttat ttgcgctgtt attcgccagt gaaaaagcgt tcaatctttt
    23641 tcactgaatt aataattcgc ataatcccaa tggcgcaggc caccgaaata atcagaacaa
    23701 gccatgagat aaatatactc atgcgatatt tcccagctta tacggttcaa tatgttcccc
    23761 gcattctgcg gcacagatca gctcggaaag ttcgttaagt gcatccagat catcagcgta
    23821 aaaagccacg tcatacagac ttcggattgc tctggtcaat gagtcacggg ccgcacgttc
    23881 agcatgagcg cctgatgcac ttaagcgaaa ataaaaacgc tcaagtgctt tgttaatgag
    23941 agttttatat tctttgccca tcgcaacgcc ctttaatctg ctttctgaat ttcagcttct
    24001 gaatccatac aaataatttc gatatagggt tcatcgccat taatcttacg tgctttttca
    24061 gcttcgctaa tgattccgtg tacagtctgg tacggaagtt ctacggtcag gcgcgtgccg
    24121 ttcagataaa cgtaagtagc tgcatttttt tctgatggga caactccatc aatagctgat
    24181 gcgcgcaata acagttcacc gcgaaaatca ataaaacgga taaatacacc ttgtgcatgc
    24241 tctttggtca taacgcacct gttataaatc agtctgttta ataaaacttt gcccgcgaag
    24301 cagacgatca accgtgcgaa gtgcttcgta caatgtgaaa tcctgtccaa actgattgtc
    24361 gccgttgctc agagcaaaaa tgcggtttcc ggtaaatggg ttgcgttggc atctgtgaac
    24421 cacgattcca gctttttcaa tcagccaggt gtgttcgcca atttgtttta ctgtatggcc
    24481 atctggcgtt gcgtgtgtct cgctcaggct gtagcggatg ttgctgcgtg atgcgctggt
    24541 agcgaaacgg ttagcttggc gttctgttcc ggtacaaaaa ttacggcgtt gcttcagcat
    24601 aaaatgacac ctcgttattt tgtcatctgc acgtatttct ctgcgtttct gatggttttc
    24661 aggaaaattg cgaagagatt tactgtacgt tttgagtttt tttcttcttg gttgatggga
    24721 agggctgctc tgtcagcctg ccttttcact gcattaacag tttgattggt acgcttcgcg
    24781 taatctttca ggctttcttc gagtaccggt aatccatgct catcgcggta tgggtagaac
    24841 gccgccaaac gctcaaaatc cgcttgctcg tatgtgttca agagctttgt catgatgtga
    24901 taacctgttc agtctgtgtt tatttgttgc taaaagtcgt ctctaggcga cttttagggt
    24961 taatttagtc gtctggagac taccatgtca agtgggtatg aaaaaaaact gaaagagata
    25021 cggaaaagtg aagggttaac tcaagctgag tttgctgatg ttactgggat aaatctcgga
    25081 actataaaga attatgagag cggtaaaaga gaggttggtt taagcgttgt tgatcgcgta
    25141 attaattcta aggatttcga aaaatacact atatggctta tgacgggaaa aacaaatgag
    25201 gctgctgggc agatcagtcc ttctctctcc cctgatgggc cagaaaacac atcgtcttct
    25261 caaaaatccc gcaagactgg cacacagccc ggctaatcat ggaacgctgg gggcatggtg
    25321 gtcttgtaac gctggggctt cacgaatgag cataaaatca attccgggag ggtatcttct
    25381 tgacatgcgt cctgaggggc gtaaaggcaa acgcattcgc aaaaaattta aaacgaaatc
    25441 ggatgcagtt ttatatgagc ggtgggtgct ggcgcaacag cataacaatg agtggaaagg
    25501 aaactccatt gatcgccgtc cgctgtcggt gcttattgac ttgtggtgga aataccacgg
    25561 ccagctaatg aagtcagggc ataacacgcg ccttaaattg ctgcgcttga gtgaggcaat
    25621 ggatgacccg tgtgtgcata aacttaatac aacgatgctc accgagctac gtgtgtccag
    25681 gatagagcag gggatacagc ccagcaccat taatcgagag attggggcgt taagcgcgat
    25741 gtttaccgca ctcatctcat ccggccattt tcttaacgat aaccccgttc aaggccttaa
    25801 aggaatgaag gttaacgagc gcgaaatggg atacctgagt aagtctgaat gtgttcagtt
    25861 gctggatgca ctggctgaaa atcccgatga acggctggct gtcgaaatcc ttctgtcgac
    25921 cggggcgcga tggggcgagg tagcggcact ggagcagcgc cgtgttcttc attgtcgaat
    25981 cactttttca aaaacgaaga acagcaaaaa ccgtaccgtt cctatttctg aaagcctgtt
    26041 tgaaaagatc aaaaaacggg gcgggaaact ggtgtttccg acgctggatt attcattggt
    26101 tcgcgatgtc atcaaaacgg tcgcacctga tgttcctgac ggccaggctg ttcatgcgct
    26161 gcgccacacc ttcgccagtc atttcatgat gaacggcggc aatattctga ccctccagaa
    26221 aattttgggg cacgcaaaga ttcagacaac gatgatttat gcccatcttg cgccggatta
    26281 cctgcaggat gcggtgagat ttaatcccct tggagatgcc ttatatgaag cctcttaaat
    26341 ttgatgattt tccatatgca gacaggtctc ttaatgataa tgaacgtggg catttgcagg
    26401 ttctgcaaca gatttcgccc aaattattta ttcaattcct caaagataga ggagccagaa
    26461 cctcttgtct ctcgtgtggg cgcccagatt tgtttattcc acatacggtt gttcacggca
    26521 cagaccctga acttgatgat tatgatgatt caaatgattg ggaatatgtc actcccatac
    26581 acaaggagaa cgagcccatt aacatctaca atactcggta tgaagtgtca tgttcttatt
    26641 gcggcttcac atccacatat acagcccaca cagttgtttg ttgggcaaga gacaaaggat
    26701 atatagtttg ggagggaatc tgagtggcat atgctaaaag tgagcatggc ataaccctta
    26761 ttcgtgagga cattggcaat tctttcgatg ggggaggcgg tgatagtgga ggcggaatgt
    26821 cagataaact tgaaaggcga attgagcggc tcgaaggtga tttatcgcta acaagaaacg
    26881 accttgcgac gcttgctgaa cgcactacaa acctctcaac caaagccgat gttggtgagg
    26941 tgaaaggtga gctcaaagca gacattgctc atctgaaagg tgatcttaaa tgcgatattg
    27001 cgaatctgaa aggtgagctt aaatctgata cagctcacct gaaagagcag ctcaaatcag
    27061 acattaacag cctgaagggt gagcttaccg aagcgatgga taaacgcttt gacaagatta
    27121 tggatgagat gaatcggcgg tttgacaagg ttgatgataa tacgaagtgg cgttggagtg
    27181 gcattattgt gccagtatgc acaaccattt tcacggcggc ggttgctgta tttgttgcta
    27241 aatttgttgg ctgatgatcc acaaattgac cacatctctg ttatttgttg tggttggctg
    27301 tgtttttgtg tgtctgtaag tctttgataa ttatctaact tgttgatttt tgtttgtgtt
    27361 tatggccgct ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc
    27421 catgacactt cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa
    27481 atattcgctg gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt
    27541 gcgccggtga acaatggatc tcgccaggtg actggtttac tcctcgtggc gaactgttcg
    27601 tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta
    27661 tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg
    27721 gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac
    27781 tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac
    27841 tcttacgttt cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat
    27901 tagggattat ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt
    27961 tacgtcagct gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct
    28021 ggctgatgct ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga
    28081 atatgttagc acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc
    28141 gcaatgtgct ggtggccgcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg
    28201 ctatcggctt tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc
    28261 atcgcgtatt acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata
    28321 ttgtagcgcg cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt
    28381 tgggtgcgcc ggtgtttatc tggttattgt taaaagcaag acgttagccg caaaaagacg
    28441 gtctatgatt aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc
    28501 cattctgacg accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc
    28561 tggtaatgtg ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga
    28621 gcagttggag aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc
    28681 gtgcaaccag tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac
    28741 caccacatgg ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg
    28801 ccatccgctg tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag
    28861 cggattctat gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt
    28921 atggaatttt gagaaatttt tagttggcag ggacgggttg gtcatccagc gtttttcccc
    28981 ggatatgacg cctgaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata
    29041 atgtctattg tgatgcagtt acaggatgtt gcggaaacta cccgcctggg gccgctttct
    29101 ggcgaggttc gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt
    29161 accttgcttg cacgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg
    29221 caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta cctttcacaa
    29281 cagcagacgc cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat
    29341 aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc
    29401 ggacgtagca ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg
    29461 gtgctgcaaa tcacaccaca agccaatccc gcaggtcaat tgctgcttct tgatgagccg
    29521 atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt
    29581 cagcaaggac tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg
    29641 catcgggcgt ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg
    29701 ctcacgccgc caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa
    29761 ggtcacagaa tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt
    29821 acgacgctaa attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc
    29881 ttattttgat cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa
    29941 atgccagact ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct
    30001 ggcatggcac gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat
    30061 ttgtggttgt aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac
    30121 aagcctctat cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt
    30181 tcaaaacggg ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat
    30241 ttatccacgc ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc
    30301 agaaaaattt ctggcaggcg agacgaattt agtatgtcga aaaatggacg gcgatgatac
    30361 atcgccgtcc atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg
    30421 attattcgtt tataatcagg ataatctaaa aaaaggaccc ctgaaaaagt ggttaacatg
    30481 gaacaaatga aagttactcc ttatgtaatt gttatgtctt atggcctcat cccttttagc
    30541 cggatactga aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc
    30601 tttatcattc tgattgctat ttcctgccga taagagataa ccagcaagac cttgtcggcg
    30661 ttgagttaat tacccatttc tccagtgaag atggtacggt tcgtatcccg acgagtcggg
    30721 ttatagcgca acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac
    30781 taaaatcatg ccaacatttt tttattcaac ataaactctt tgcctggctt aatttaacgc
    30841 cacaagtggc tacattatta ttagagaggg ataattacgc aggcgaacta ttaaaatatc
    30901 cgtttattga actgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc
    30961 gcgatctttt atccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta
    31021 atagcacaat gaaagcggtt tttgatggtc ttttcacccg cgtcatgctg gataaaagtt
    31081 ttattcagca acaaatcacg catcgctcct tcgaaccgtt tattcgtgcg atccaggcgc
    31141 aaatttcccc ttgttgcaat tgcatcatcg cggggggcat tgatacgaca gaaatattgg
    31201 cacagattac ccccttcgat ttccatgctc tccagggatg cctgtggcct gccgttccta
    31261 taaatcagat aacgacgttg gttcagcgat aaccctcctg tttgctggtg tttaagacga
    31321 gagtaaccgt ctacactatt aaacaggagg atctatgacc ctgtctttta ttacccgctg
    31381 gcgcgatgaa ttgccagcaa cctatacagc actttcccct acgcctttaa ataatgcccg
    31441 gctgatttgg cataataccg aactcgctaa cacgctgagt attccatcgt cgctgtttaa
    31501 aaatggcgca ggtgtctggg gcggcgaaac cctactgcct ggtatgtcac cactggcgca
    31561 ggtttacagt ggtcatcagt tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggtat
    31621 tttactcggc gaacaactgc ttgctgatgg cactacaatg gactggcatc tgaaaggtgc
    31681 tggcctgacg ccttattcgc gaatgggtga tggacgggcg gttttacgtt cgacgatacg
    31741 agaaagtctc gccagtgagg cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag
    31801 tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat
    31861 gcgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa catttttact atcgccgcga
    31921 gccggaaaag gttcgtcagt tagctgactt tgccattcgt cattactggt catatcttga
    31981 agatgatgag gacaaatacc gcctctggtt tagcgatgtt gtcgcacgta ccgcatcgtt
    32041 aattgcccaa tggcagacgg tcggctttgc tcatggggtg atgaataccg ataacatgtc
    32101 gctgctgggg ctgacgcttg attacgggcc gtttggtttt ctggatgatt acgaacccgg
    32161 ttttatttgt aatcactcgg atcatcaagg gcgttatagc tttgataatc aacctgctgt
    32221 cgcgttgtgg aatttacagc gtctggcgca gacattgtca ccatttgttg ccgtagatgc
    32281 cctgaatgag gccctggaca gctatcagca ggttttgttg acgcattatg gacaacggat
    32341 gcggcagaaa ctgggcttca tgacggagca aaaagaggat aacgcgctac tgaatgaatt
    32401 attcagtctg atggcgcgag agcgcagcga ttatacccgc acattccgca tgctgagtct
    32461 gaccgagcag cacagcgcgg cgtcaccgct acgtgatgag tttattgatc gtgcggcatt
    32521 tgatgactgg tttgcccgtt atcgggggcg tttgcaacaa gacgaggtta gcgatagtga
    32581 gcgtcagcag ctgatgcaaa gcgttaaccc agctctggtg ttgcgcaact ggttggcgca
    32641 acgggcgatt gaagcggcag aaaagggtga tatgacggaa ttgcaccgcc tgcatgaggc
    32701 gttgcgaaat cctttcagcg acagagctga tgactatgtc agtcgtccac ctgactgggg
    32761 taaacggctg gaagtcagct gctcgagtta aaggctactt ggtcaacagc agcttgccag
    32821 cctgagtttt acggagcaga tattcttgcc cgtcatgatc gataatcagt ttgccatccg
    32881 gacctaacag tgtctggctg gaaattcgcc gaataggctg cggggaagcg gtcttgtgat
    32941 tggcatccgt ttcaggcgtg agttttctgc tatccgtata acgcataaaa attaataata
    33001 attgatataa caatgattct tattatcaat attatggctg accagagcaa gcagtaatgt
    33061 gctgagaaga caacaagttg cgcagaaatg cccatcccat caggaatggg cacgcattca
    33121 gaagcgggta tctaccgcag aggcaagttt ttcgaccagg cgttcggtat cttcccagcc
    33181 cagacacggg tcggtaatgg attgaccgta agtgagcggc tgaccgccga cgattttttg
    33241 cgttccttcg cgcaggaaac tttccgccat aattccagca atcgccgtag agccattgcg
    33301 gatttgctga caaatatcct cacaaacttc taactggcga cggtgctgct tctggcagtt
    33361 accgtggctg aaatccacca ccagatgttc aggtaaatca aactcgtgca gcgtatcgca
    33421 ggctgcggcg atatcatcgg catgataatt cggttttttg ccgccacgca taataatgtg
    33481 gccatacggg ttgccgctgg tctgatagat ggtcatctga ccatttttgt ctggcgagag
    33541 aaacatatgg ctggcgcggg ctgcgcggat agcatccacg gcaatccgcg agttgccatc
    33601 ggtaccattt ttaaaaccta ccggacagga gagtgccgaa gccatttcac ggtggatctg
    33661 actttcggta gtacgtgcgc caatcgcgcc ccaactgatt aaatcagcaa taaactgacc
    33721 ggtcaccata tcgaggaact cagtcgcggt agggacaccc agctcattta cctgtaaaag
    33781 taatttgcgc gccatctcca gaccgtgatt tacccgatag ctgccgttta aatccggatc
    33841 ggagattagc cctttccagc cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac
    33901 gatttccagc cgtgactggt actggttgcg cagcgactgc agacgggtgg cgtactccat
    33961 tgcagcggtg agatcgtgga tcgagcaggg gccaataatg accaacagtc gcttatcttc
    34021 accattcagt attttttcaa ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg
    34081 cgttacggga taccgtagcg cgagttcggc gggcgttacc aggctctcaa tacgcgcagt
    34141 acggagttcg tcagttctgt tcattacaag tctcagaaat ttgttgcttc tacggcagga
    34201 ctaccgggaa gtgatgcgca tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg
    34261 tgtcacacct cgccgggtgg ttagcatgat aacaaaaaaa taagctaatg cactagttct
    34321 ctagtacatt cggcggctaa gccccatgat atcgaggagc ttggtggcaa tctcttctac
    34381 cgaataattg gtactgttaa tccacgggat ctgattttta cggtacaacg cttctacttc
    34441 cgcgacttcc atcctgcact gacgaagcga ggcatagcgg ctgttctccc gacgttcctc
    34501 gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg aacaatttat gctgaagcgg
    34561 tttgagcgac gcgggtagca ccagattgtc catatcgtcg gcaataaagg ggtagtttgc
    34621 cgcgcggata ccaaactgca ttgccagata cagactggtg ggggttttac cacagcgaga
    34681 aacaccgagc aggatcacct gagcctggtc cagattgcgc aacgaaatgc cgtcatcgtg
    34741 ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat ttattgagat tattagggtt
    34801 aaggccatgg gtacgatgag caatcggcgt tggatccagt ttcatctctt gttgtagcgg
    34861 ggcaaccagc gcctgaacaa tatcctggca aaagccttca ctttgcaaga tgatggcgcg
    34921 aatctccggc aacacgatgg agtagaagac cagcgggcgc acacctgtct ggtgataaat
    34981 tgcgtcaatc tgatccttca ctgcccgtgc acggctctca ttttcgacaa acggcagcgt
    35041 aatgctgctg atagtgacgg gaaattgtga cattactgcg tgtcctaata cctccgcagt
    35101 tattgccgta ccatcagaaa tataaaaaac gtggcgatca acagcattat ccattttgtt
    35161 cttcccgtga tgcagacata attctatgaa agcataaatt aaaaacgcac agaagcgtag
    35221 gacgtaatgt ctggtttata aaatgaacct tcaattttat tttttatgaa aacagcattt
    35281 catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat agagcaggtt
    35341 tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga acgattcacc
    35401 gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt tctcaaaccg
    35461 ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg ctggtgcttt
    35521 ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat gcctccctgg
    35581 gtgaaatgat tactaacctt tccggaatgg gtgtttccgt tccgaatggt ttcgccacaa
    35641 ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc atttatgaac
    35701 tgctggataa aacggatatt gacgatgtta cccagcttgc gaaagcgggc gcgcaaatcc
    35761 gccagtggat tatcgacact cccttccagc ctgagctgga aaatgccatc cgcgaagctt
    35821 atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtgcgctcc tccgctactg
    35881 cagaagatat gccggacgct tcctttgccg gtcagcagga aaccttcctc aacgttcagg
    35941 gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt aacgatcgcg
    36001 ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc tccgccggtg
    36061 ttcaacggat ggtgcgctct gacctcgcat cttctggcgt gatgttctcc attgataccg
    36121 aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggt gaaatggtcg
    36181 tgcagggcgc ggttaacccg gatgagtttt acgtccataa accgacactt gcagcgaatc
    36241 gcccggctat cgtgcgtcgc accatggggt cgaaaaaaat ccgcatggtt tacgcgccga
    36301 cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag cgtgacatct
    36361 tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa attgagaaac
    36421 actacggtcg cccgatggat attgagtggg cgaaagatgg ccacaccggc aaactgttca
    36481 ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag cgttatacgc
    36541 tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc atcggcgcgg
    36601 gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct ggcgacgtgc
    36661 tggtcactga catgaccgac ccggactggg aaccgatcat gaagaaagca tctgccatcg
    36721 tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa ctgggcattc
    36781 cggcggtagt gggctgtggt gatgcaacag aacggatgaa agacggtgag aacgtcactg
    36841 tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa tttagcgtga
    36901 aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg aacgtcggta
    36961 acccggaccg tgctttcgac ttcgcctgcc tgccgaacga aggcgtgggc cttgcgcgtc
    37021 tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt gagtttgacg
    37081 atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt gattctccgc
    37141 gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc gcgttttatc
    37201 cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc aacctggtcg
    37261 gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt ggcgcgggcc
    37321 gctatgtttc cgacagcttc cgcgactgct tcgcgctgga gtgtgaagca gtgaaacgag
    37381 tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg cgtaccgtag
    37441 atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt ggcgagaacg
    37501 ggctgaaaat catcatgatg tgtgaaattc cgtccaacgc cttgctggca gagcagttcc
    37561 tcgaatattt cgacggcttc tcaattggct caaacgatat gacacagctg gcgctcggtc
    37621 tggatcgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat gcggtgaaag
    37681 cattgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc gggatttgcg
    37741 gtcagggtcc gtccgaccat gaagactttg ccgcatggtt gatggaagag gggatcgata
    37801 gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct gaactgaaga
    37861 aataaaataa atccccggcg gcgttcagtc gccgggttta tgtgatcccc gaagataaaa
    37921 cttattcaat ctcttcacag acatcctgcg ttaatcgccg cataatatct tttcttaaca
    37981 aaaacttttg tattttacct gaggcagtgc gcggtagttt ttcgattacc acgatatgtt
    38041 caggatattt atattttgcg acccgtttac ggctaaaaaa agccaccacc tcttccagcg
    38101 ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca cctaaacgtt
    38161 catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat aaaatatctt
    38221 ccacttcacg gctgctaata ttttcgccgc cgcggacaat aatatctttt ttgcgtccgg
    38281 taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa taccagcctt
    38341 cttcatccag cgcacgggcg gttaattcag gttcatcaaa atatcccata aacacattgg
    38401 ggccacgcga ggcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt gcgtcatcga
    38461 ccactttaat ctctacacct gcggcagcgt aaccatcggt gtgcataaag cgcgacaaag
    38521 gatcatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa acacttaata
    38581 atttaatgcc gagctgctgg cattcacgcg ccactttttt ggggattgtg gttccgccgc
    38641 aaagaaagaa acgcagcgct gacaggtccg cgggttgttt ctctagtaca ttcaaaagat
    38701 catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca agcagcgcga
    38761 gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt aagaatggtg
    38821 ccgttacgcc atgcagaaag cccgttgcgt ggccgagtgg cgcaggcatc ataaagacat
    38881 cctgccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga atattgttat
    38941 gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc accgcagcta
    39001 attcatcgcc gtgggtcgtt atcgccgtgg tcagtggggt attgtcggcg ataatctgac
    39061 ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt tgttgtagtt
    39121 gtggaagctg attttgcagc ggcaggatta aatctaccgg gcgcgtttgt ttaaacaacg
    39181 tcggtgcaaa gaacattttt gcctgacact tattgagcac ccacaccagt tctgcttccc
    39241 gccaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca agatagataa
    39301 cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat tcaataccct
    39361 tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta taggtgtacg
    39421 atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg gtctgctgcc
    39481 agtaatcggc cagcgaagca tcgccccata gcccttgctg acgatacgcc gcacgacgtt
    39541 gttcgttgaa cgttaatgtc actttcatgt tggactctcg aaacagaaca tcaggcccga
    39601 gatgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga aggtgcctgc
    39661 gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg tgccgcactc
    39721 cagacaaccg gcagaatcaa aatgaatgtt gccggcgtca tcctgcttat aaagtccggc
    39781 agggcaggct ttcattaatt tacggaattc attgatatcg ggatttgccg ccagaatgat
    39841 atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt cgacgttaac
    39901 cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag caagttgatc
    39961 agcccaattt tcttcgcatg tcccatgatc attttgcgta ccggctggtt tggtttgccg
    40021 tcaatggtga acatctcgtt catgatgtcg gcgaccattc gtgggtattg gctaaacagg
    40081 cgcgggtttt ccatcagcgc cgggatcttg cggaaatgct gcatatcgcg catgatgcag
    40141 ctttgttcca gctcacgttt gtattgcgcc agactgctgg cggagaaatc ctcgcgttct
    40201 ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa atccatgcca
    40261 cgaactgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat catcacgcca
    40321 tcgttaacca tctgcggcac cattgccaga ccgccttctg gcaccatatg cgcggaatat
    40381 tcaagcagtt tgccgccgct aattagtggg cgaatggcgg ggtgttgttt aaaatcttcc
    40441 agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca aaccagcccc
    40501 aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc gtcagaaggg
    40561 gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg atcgttgatc
    40621 tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg cggatcggaa
    40681 gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc cagaatcacc
    40741 acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt tccttcacga
    40801 accagcgcat cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc ggcttgttcc
    40861 atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg cggaacatct
    40921 ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa ggagattttc
    40981 tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat gattgcttca
    41041 agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact gtcgccgcgt
    41101 tctatcacca ggacatccag cccggctcgc gccatgacca gtgcggcaac gctaccagca
    41161 acgcccgcac cgaccacaat ggcatcaaat ttgtcttccg acatgcgtta tgctcccttc
    41221 ccgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca actggctaat
    41281 cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat aattgaagat
    41341 tggcgcattt ttatctttat tgatggcgac aatcactttt gcgccgttgc cgccaaccat
    41401 atgctggatc tgcccggaga tccccagcgt caggtagaga tcggatttca gcaacacgcc
    41461 ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa ttggacgtga
    41521 acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca ggtcatcctg
    41581 cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt ccacgctgct
    41641 tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa cataaggtac
    41701 cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa caccgggtgc
    41761 aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta acccgccata
    41821 catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg catcgttcac
    41881 cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac gtttcgtcgc
    41941 ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc tttcggcgta
    42001 attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt ttggaccata
    42061 aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct gttggcccca
    42121 ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa agacccagac
    42181 gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct ttcttcagat
    42241 gttccgccag ctcggcaatg gcctccggcg aatcgttatc gataatgatg tgcttacgtt
    42301 ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc tggctccagt
    42361 caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga atggctttca
    42421 tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct ggaacagaga
    42481 gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta ttgccctgac
    42541 gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg accagcaagc
    42601 caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa tcgaagccga
    42661 tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca tgttcaagtt
    42721 gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct ttgcgcactt
    42781 tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg tcatctgttg
    42841 cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg gcgtcggcat
    42901 tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca ggcaccagct
    42961 taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac catctccgat
    43021 ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt cgacggagca
    43081 agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tcgctaaaac aaaagtgcaa
    43141 tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta aataatcttg
    43201 tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt atccggcata
    43261 tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct gttttgataa
    43321 cgccagtgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt ttgcatttag
    43381 cgatgatcca aaatgggagt ccggacatca cgttcatgac aatgaaaccg agttgattta
    43441 cgtcaagaaa ggggttgcaa ggtttaccat cgattcttcg ttatatgtcg cacatgcgga
    43501 tgacattgtg gtgatagaac gcggcaggct acatgcggtg gcctctgacg ttaacgatcc
    43561 ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg aggaaaatca
    43621 gctactgcaa ccgcattctt gtccggtaat tgccgcgggg cagggaaaag aagtcataaa
    43681 aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc aaacatcttc
    43741 gttatgggat gcatttgcct atacgttagc cattctttac tacgaaaatt ttaaaaatgc
    43801 tta
//
LOCUS       DAZFRJ010000016        41929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000016 DAZFRJ010000000
VERSION     DAZFRJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.016"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHKUN_000815"
     CDS             complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHKUN_000815"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO6659125.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1839..3488
                     /locus_tag="ACHKUN_000816"
     CDS             1839..3488
                     /locus_tag="ACHKUN_000816"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6659126.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3539..4141)
                     /locus_tag="ACHKUN_000817"
     CDS             complement(3539..4141)
                     /locus_tag="ACHKUN_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO6659127.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4661..5560
                     /locus_tag="ACHKUN_000818"
     CDS             4661..5560
                     /locus_tag="ACHKUN_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6659128.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5609..6622
                     /gene="yhjD"
                     /locus_tag="ACHKUN_000819"
     CDS             5609..6622
                     /gene="yhjD"
                     /locus_tag="ACHKUN_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO6659129.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7033..8355
                     /locus_tag="ACHKUN_000820"
     CDS             7033..8355
                     /locus_tag="ACHKUN_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6659130.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8589..10649)
                     /locus_tag="ACHKUN_000821"
     CDS             complement(8589..10649)
                     /locus_tag="ACHKUN_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6659131.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10719..11486)
                     /gene="pdeH"
                     /locus_tag="ACHKUN_000822"
     CDS             complement(10719..11486)
                     /gene="pdeH"
                     /locus_tag="ACHKUN_000822"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO6659132.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11718..12647
                     /gene="kdgK"
                     /locus_tag="ACHKUN_000823"
     CDS             11718..12647
                     /gene="kdgK"
                     /locus_tag="ACHKUN_000823"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO6659133.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12743..14239)
                     /locus_tag="ACHKUN_000824"
     CDS             complement(12743..14239)
                     /locus_tag="ACHKUN_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6659134.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14459..15745)
                     /gene="dctA"
                     /locus_tag="ACHKUN_000825"
     CDS             complement(14459..15745)
                     /gene="dctA"
                     /locus_tag="ACHKUN_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO6659135.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15928..17916)
                     /gene="hmsP"
                     /locus_tag="ACHKUN_000826"
     CDS             complement(15928..17916)
                     /gene="hmsP"
                     /locus_tag="ACHKUN_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO6659136.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17998..21471)
                     /gene="bcsC"
                     /locus_tag="ACHKUN_000827"
     CDS             complement(17998..21471)
                     /gene="bcsC"
                     /locus_tag="ACHKUN_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO6659137.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21453..22559)
                     /gene="bcsZ"
                     /locus_tag="ACHKUN_000828"
     CDS             complement(21453..22559)
                     /gene="bcsZ"
                     /locus_tag="ACHKUN_000828"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO6659138.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22566..24905)
                     /gene="bcsB"
                     /locus_tag="ACHKUN_000829"
     CDS             complement(22566..24905)
                     /gene="bcsB"
                     /locus_tag="ACHKUN_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO6659139.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24916..27534)
                     /gene="bcsA"
                     /locus_tag="ACHKUN_000830"
     CDS             complement(24916..27534)
                     /gene="bcsA"
                     /locus_tag="ACHKUN_000830"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO6659140.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27531..28283)
                     /gene="bcsQ"
                     /locus_tag="ACHKUN_000831"
     CDS             complement(27531..28283)
                     /gene="bcsQ"
                     /locus_tag="ACHKUN_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO6659141.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28295..28483)
                     /gene="bcsR"
                     /locus_tag="ACHKUN_000832"
     CDS             complement(28295..28483)
                     /gene="bcsR"
                     /locus_tag="ACHKUN_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO6659142.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28756..30327
                     /gene="bcsE"
                     /locus_tag="ACHKUN_000833"
     CDS             28756..30327
                     /gene="bcsE"
                     /locus_tag="ACHKUN_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO6659143.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30324..30515
                     /gene="bcsF"
                     /locus_tag="ACHKUN_000834"
     CDS             30324..30515
                     /gene="bcsF"
                     /locus_tag="ACHKUN_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO6659144.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30512..32191
                     /gene="bcsG"
                     /locus_tag="ACHKUN_000835"
     CDS             30512..32191
                     /gene="bcsG"
                     /locus_tag="ACHKUN_000835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO6659145.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32278..32385)
                     /locus_tag="ACHKUN_000836"
     CDS             complement(32278..32385)
                     /locus_tag="ACHKUN_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6659146.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32761..32868)
                     /locus_tag="ACHKUN_000837"
     CDS             complement(32761..32868)
                     /locus_tag="ACHKUN_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6659147.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33244..33351)
                     /locus_tag="ACHKUN_000838"
     CDS             complement(33244..33351)
                     /locus_tag="ACHKUN_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6659148.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33727..33834)
                     /locus_tag="ACHKUN_000839"
     CDS             complement(33727..33834)
                     /locus_tag="ACHKUN_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6659149.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            34310..35581
                     /locus_tag="ACHKUN_000840"
     CDS             34310..35581
                     /locus_tag="ACHKUN_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO6659150.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(35611..36615)
                     /gene="dppF"
                     /locus_tag="ACHKUN_000841"
     CDS             complement(35611..36615)
                     /gene="dppF"
                     /locus_tag="ACHKUN_000841"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO6659151.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(36612..37595)
                     /gene="dppD"
                     /locus_tag="ACHKUN_000842"
     CDS             complement(36612..37595)
                     /gene="dppD"
                     /locus_tag="ACHKUN_000842"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO6659152.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(37606..38508)
                     /gene="dppC"
                     /locus_tag="ACHKUN_000843"
     CDS             complement(37606..38508)
                     /gene="dppC"
                     /locus_tag="ACHKUN_000843"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO6659153.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(38518..39537)
                     /gene="dppB"
                     /locus_tag="ACHKUN_000844"
     CDS             complement(38518..39537)
                     /gene="dppB"
                     /locus_tag="ACHKUN_000844"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO6659154.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(39688..41295)
                     /gene="dppA"
                     /locus_tag="ACHKUN_000845"
     CDS             complement(39688..41295)
                     /gene="dppA"
                     /locus_tag="ACHKUN_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO6659155.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(41647..>41929)
                     /locus_tag="ACHKUN_000846"
     CDS             complement(41647..>41929)
                     /locus_tag="ACHKUN_000846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659156.1"
                     /translation="SVASGNGTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVT
                     ETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         9770 a        11529 c        10585 g        10045 t
ORIGIN      
        1 atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg
       61 cgtgtacacc ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg
      121 cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac
      181 gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg
      241 acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat
      301 ttccccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc cgccgagaat
      361 aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg
      421 ttgcgccgtc agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt
      481 aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg
      541 cggataaact tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat
      601 ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac ctgcctgatc
      661 ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac
      721 cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc
      781 gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa
      841 acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt
      901 ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta
      961 atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac
     1021 cttacccgcc cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac
     1081 ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg
     1141 cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt
     1201 cgcgccagga atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca
     1261 tcctttttcg cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac
     1321 atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata
     1381 gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct
     1441 tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc
     1501 tgcgagacaa agccatcata aaaaacaggg agatacgctt tttgatagga atcaatcaat
     1561 tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca
     1621 tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc
     1681 cgtattttta taaattccat tgatattagt gcgttatatt ttttgacaag ttaataaacg
     1741 tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag
     1801 cccgataatt agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa
     1861 accctaatcc agacgaactg atgatcgagg tcgatctctg ctatgaactg gacccgtatg
     1921 aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc
     1981 ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt
     2041 cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacccaaa atggacccgc
     2101 tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt
     2161 ttgttgaaaa tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc
     2221 aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg
     2281 atcacattcc gtggtcttct ctgctggcgc taccgcagtc atatattgtc ccgggcggcc
     2341 gttttagcga aacctactat tgggattcct atttcaccat gctggggctg gcggaaagtg
     2401 gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactatg
     2461 gtcacatccc caacggcaac cgcacctatt atttgagccg atcgcaacca ccggtttttg
     2521 cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc
     2581 accttaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg
     2641 cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttat tgggacgatc
     2701 gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc
     2761 gcccgcccaa cgaggtgtac cgcgatttac gcgcgggagc ggcctcaggt tgggattact
     2821 cttcccgttg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc
     2881 ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc
     2941 tgaaaggcga gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc cgtcgcgatg
     3001 cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac
     3061 gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggcatggcga
     3121 accatgaaca ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg acacctggcg
     3181 ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaat ggctgggcac
     3241 cgttacaatg gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg
     3301 aaatcgcgcg cagctggctg aaaacggtga atcagttcta tctggaacag cacaaaatga
     3361 tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt
     3421 tgcaggatgg gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg
     3481 aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc
     3541 aggaggagat atttaacatc attgccgcct gggtgcgatt tttcacttcc agacggcgat
     3601 acagggattc cagatgcgct tttaccgttc cggtactgat attcagcgct ctgccgatct
     3661 ccttatttga ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg gcgcttaacg
     3721 atttgagatc tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc agaaacatca
     3781 tccccatcgc cgcactattt accgccaacg caaatgtctc gacggttgaa tcacgaggca
     3841 caatggccag cacattaaaa tggataactt cctgtaacca ccgtttattg caatccgtcg
     3901 ccgtgattaa caccttaacc tcaggaaact gcaccacggt tttttgcagc aaccagtagc
     3961 aaaactcacc atcctgatcg ccatcgagca taactaaggc ttcagggtaa ctttccagct
     4021 tttgccataa ctcgtctgcc tgactggccc cctgaatact cactcctgga atacgctgct
     4081 gtaaactgat tttcattcca tgaataaata ttgactgcct gtcaaacatg actatttgca
     4141 taactgaatc tccacctgaa tacgttaaaa agacttaagt agtggaaggg tattacccgc
     4201 gagaaaaaat aagaattcgc catttggcgg tggccattct acagagatga cgcgtagaaa
     4261 atagttaccg atataaatag ttacagctaa acgcctgaaa ttacatgtcg agggcactat
     4321 ttaaaacaat tttgaggatt tccttatatt ggtggttagt acgcatgcaa ttaaaaatga
     4381 aactccgcga ccacaagcaa aaataacaaa cggcaatgag acaaaaataa gcacaaatag
     4441 ccaacacgtc ctctattcac tttaaaggga atcgctgaaa aatacgctct gtttaggggg
     4501 attcaccttt ctcagaaagc tattccgccc ttttcctgct gagaaatcgc cacattcgtc
     4561 atgacaacat tgtgaaaccc ggcattagat gttagaaaaa acaataaaca atgcgatatg
     4621 cgcgttactg tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa ttcacgcaat
     4681 gcagttgttc atcaaagtcg cggagctgga aagtttttcc cgcgcggcgg atttctttgc
     4741 tttgccaaaa ggaagtgttt cgcgccagat acaggcactg gaacatcaac ttggcaccca
     4801 gcttctccag cgcaccacgc gacgggtcaa actcacgcca gaaggcatga cctattatca
     4861 acgagcaaaa gatgtgttga gtaatctcag cgaactggac ggtctgtttc aacaggatgc
     4921 caccagtatc agcggtaaat tacgcatcga catcccgcca ggaatcgcga aaagcctgtt
     4981 actgccgcgc ctgtcggaat ttctctatct gcatccggga attgagctgg aactgagtag
     5041 ccatgaccgt ccggtagata ttcttcatga tggttttgat tgcgtgatac gcactggcgc
     5101 gttaccggaa gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg tcaactgtgc
     5161 cagtccgcac tatctgacgc gctttggtta tccgcaaagc cccgatgatc tgacttcaca
     5221 cgcaatagtg cgttatacac cgcacctggg tgtacatccg ttaggttttg aggttgccag
     5281 cgttaatggc gtccagtggt ttaagtctgg cggcatgttg acggtaaaca gtagcgaaaa
     5341 ctatctcgcc gccggtcttg ccggtctggg gattattcag atcccgcgca ttgccgtgcg
     5401 cgaagccctg cgtgccgggc ggcttattga agtgttgcca ggctaccgtg ccgagccgct
     5461 ctccctttcg ctggtttacc cgcagcgtcg ggagctttcc cggcgtgtaa acctgtttat
     5521 gcagtggctg gctggcgtaa tgaaagagca cctggactga ccgactatac tttttaagaa
     5581 tgaccacgac agaaggacaa aagagcggat gacgcaggaa aacgagatca aacgtcccac
     5641 ccaggatctg gagcacgagc cgattaaaca gttggataat agcgagaaag gcggcaaagt
     5701 tagccaggcg ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc aaccggttat
     5761 tgcgcacctg attcgtgcga cagaacgctt taacgatcgg ctgggtaacc agtttggtgc
     5821 ggctatcacc tatttctcat ttttgtcgat gatcccgatt ttgatggtgt cgtttgccgc
     5881 agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg acaaaattct
     5941 gcaaaacatc agcgatccga cgctagccgc cacgttgaaa aacaccatca acaccgccgt
     6001 tcagcagcgt acgactgtag ggcttgtcgg cctggcggtg gcactttatt ccggcatcaa
     6061 ctggatgggt aacctgcgtg aagcgattcg tgcccagtcg cgcgatgtct gggaacgctc
     6121 gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt cactgattgg
     6181 tttgttaatt gcgctgattg tgacgctgtc gatcacctcg gttgccggtt cggcgcagca
     6241 aatgattatt agcgccctgc acctgaacag cattgagtgg ctgaaaccga cgtggcggtt
     6301 gattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttctgga tcttctggcg
     6361 gttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc tggctgctat
     6421 tggttttgaa gtgattaaaa tcgtgatgac ctacaccttg ccatcgctga tgaaatcccc
     6481 ctctggcgca gcatttggtt ccgtgctggg gctgatggca tttttctact tcttcgcccg
     6541 tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg acccgcgaat
     6601 gccagggaaa acgcagcctt aaaataggcc ggatgcggcg taaacgcctt atccggcata
     6661 cgattttatt cggcctggct ccccgtaggc cggataagat gcgccagcat cgcatccggc
     6721 tataatgcgc acataacctc ttgaaactct tccccagagc ctcttcagcc atctatttgg
     6781 gagcaaacaa tttcattcca actcataacc ccagcatata aatccagttg gtaactttta
     6841 tttaacctga aaccagtttt atccactatt tataaaatta tgtgaagcat ttcatagaag
     6901 aaaaatcact ggcctaaaca ttatcccctt tttgcctggt ttttgaccat ttccgcgatt
     6961 tgttacacat tgaaatatca cttttgctgt gcgtaatatg gctattcgtt agccaaaaaa
     7021 taagaaaaga ttatgcaagc aacagccaca acactcgacc acgagcaaga atacacgccg
     7081 atcaactcgc gtaataaagt ccttgtcgcc tctctcattg gcacggccat tgagttcttc
     7141 gacttttaca tttacgccac tgcggccgtt attgtgtttc cgcatatctt cttcccgcag
     7201 ggcgatccaa cggcagcaac gctacagtcg ctcgccacct tcgccatcgc cttcgtcgcg
     7261 cgccccattg gctctgccgt ttttggtcat tttggcgatc gcgttgggcg taaagcgacg
     7321 ctggtcgcct cgttgctaac gatggggatt tcgaccgtgg tgattggtct gctgccgggc
     7381 tatgccacga ttggtatttt cgccccgctg ctgctggcgc tggctcgatt tggtcagggt
     7441 ctgggcttag gcggtgaatg gggcggcgcg gcgctgctgg cgactgaaaa cgccccaccg
     7501 cgcaaacgtg cactgtatgg ctccttccca cagctgggcg caccgattgg cttcttcttt
     7561 gccaacggca ctttcttgct gctttcctgg ctactgaccg acgagcagtt tatgagctgg
     7621 ggctggcgtg tgccatttat cttctcggcg gtgctggtca ttatcggcct gtatgttcgc
     7681 gtgtcgctgc atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa acaggtgaag
     7741 atcccgctgg gtacgctgct gaccaaacat gttcgcgtaa ccgtactggg tacgttcatt
     7801 atgctggcaa cctatacgct gttttacatc atgacggtct actctatgac cttcagtacc
     7861 gccgccgcgc cagttgggct tggcctgccg cgtaacgaag tgttgtggat gctgatgatg
     7921 gcagttattg gttttggcgt gatggtgcca gtcgccggat tactggctga tgcctttggt
     7981 cgccgtaaaa gcatggtaat catcaccacg ctgatcatcc tgttcgcgct gttcgccttt
     8041 aacccgctgc tcggttctgg caacccgatt ctggtttttg ccttcctgct gctggggtta
     8101 agtctgatgg gactgacctt cgggccgatg ggcgcgctgt taccagagct gttcccgaca
     8161 gaagtgcgtt acaccggagc atcgttctct tacaacgtag cgtcgattct cggggcttcc
     8221 gttgcaccat atatcgcagc ctggttgcag gctaactacg ggttaggtgc ggtggggtta
     8281 tatctggcgg cgatggctgg cttgacgtta atcgccctgc tgctgaccca tgagacgcga
     8341 catcagtcgt tgtaagtatc gtgtgggcag ccaccctcac cccagccctc tccctgaaag
     8401 ggagagggag aaaagcggag cgagatgtta tatcgcgaac ataacagccc tcccccagaa
     8461 agtgaacgga aaagcggtac tcgttgtttc aacacgcaca aaacagtccc ctcgcccctt
     8521 tggggagagg gttagggtga ggggaacacg ctgctcccga actcaccagc aaacacaatc
     8581 caacctcatc acttcttcat ctgcgacaat atcgtccgac actgattagc ctccccttcg
     8641 gaaggtgaga tcagtgccag taacgccgcc gccggtgtta ccagcgttgc cagtgccgca
     8701 gcaacagcac cacgggcaat caacggcccg gctttcaccc cagcctgcgg atttttaaac
     8761 gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaattccttt actctccgga
     8821 tcaatagtca aatccagctg ttcggaagca aaacttgccg tgccggtaac gttaatcaac
     8881 gcgttctcag tatcgaaagc aaagatctgc gggcgcgcca cgccgttggc aatatccaga
     8941 ttcgccgccg cgcagttcac ccgcacctca tcatcaccaa atatcgcacc gacaatgtag
     9001 ttgccgacat tcagcccaac aatctccatc aggttgcggc tcaccagccc gtcattcatc
     9061 aacagtttca ggttgccgtt actgttgcct aaaagtgccg ccaccgagtt accgctaccg
     9121 cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc cacatcgggc
     9181 atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccctg catcggcttt
     9241 ttatcgcctt ccagatgaat attcgccgca atgctgccgc ccgccatgcc aaatttcagc
     9301 ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc actaatcggc
     9361 aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc cataacgtcc
     9421 catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg aacgcttttt
     9481 tcgcccttct tctgttcaga ccgtttcgac ttttctgccc ctttcccgga gtcaacgcca
     9541 attaacggtc ccaggtccgc cagccgcaat tgccgcgact cgacatcacc ttccagtttt
     9601 ggtcgtggct ttccggtggt gtagaccaga gaaccgtgga tatcgctatc gccaattcgc
     9661 ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc
     9721 cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga
     9781 tcacccagtg aatcgccaga aaatttaagc cgtaaatcga caccgcccat cttcattgga
     9841 tcattcacga cgccatcaaa agcaacacgg gtgttaccag agcggaaatc agcctgtacc
     9901 ggaaacggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtacccgtg
     9961 agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt
    10021 tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg cttgcctaac
    10081 ggatcaacaa aaatctccag atccgctttg cttactttgt catcaatggc gatccgccct
    10141 tgatcgaaaa gaatattatc cagccgaaac gaccatgccg acggctttgc attcgcgtct
    10201 ttgttatcat cgttggcgag attaaacgtc cagttattgt tcttttcgga gaggcgaatc
    10261 aggcgcgcgt cgggcttttc gagcttgatc cacggcagcc agaccgtttt ggtcagcagc
    10321 gccagcgggg ccagcgttgc ctctacgcgt ggcaaatgca ccatcgtgac ttcgggaata
    10381 tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg cacccagctg
    10441 cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg
    10501 aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag
    10561 tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc cactgaaatc
    10621 gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag
    10681 tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag aaccgccgta
    10741 ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc
    10801 gagttctgaa catcacgcca ctcttccggc gtttctacgc cctcgacaat caccccgcga
    10861 caatagcgat tcattagatg taaaagctga gaaaagagtg tgcgtccttc cggcgactga
    10921 cgcagcatca caaacagttc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagt
    10981 gcagagaaat ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc aaattcacac
    11041 atcgaggcaa aggttgaatc tttcggcagt cggatatgct ccaccagttc gaaacgcagc
    11101 cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta
    11161 gggccatcaa tattgaccga tgccagcagg ccgtgctcta taaagaagtc ggctttttgc
    11221 gccagcaaat caatctgctc tttcacaacc tccatccgat ggctgacggt gatttcagta
    11281 aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac caccgttaat
    11341 agctccacgg ccattaaccg tccgcatgtt tgatagatcg gctgccaggt gtaagcacgc
    11401 tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt
    11461 attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc
    11521 ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggc cggggtaaat
    11581 ttcctcactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa ataatggaac
    11641 actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac
    11701 agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc
    11761 tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg
    11821 tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta acggcgctgg
    11881 gaacggacag ttttagccag caaatgctgg acgcctggca cggcgagaac gttgatactt
    11941 ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca
    12001 ccggcgagcg tacgttctac tactggcgga acgaagccgc cgccaaattc tggctggaga
    12061 gtgagcagtc tgcggcgatt tgcgaagagc tggcgaattt cgattatctc tacctgagcg
    12121 ggattagcct ggcgatctta agcccgacca gccgcgaaaa gctgctttcc ctgctgcgcg
    12181 aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg
    12241 ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg gatatcgcct
    12301 tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg
    12361 cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc
    12421 tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtgaaactg ccgaaagaaa
    12481 aagtgatcga taccaccgca gctggcgact ctttcagcgc cggttatctg gcggtacgtt
    12541 tgacgggcgg cagtgcggaa aacgcggcga aacgcgggca cctgaccgca agtaccgtta
    12601 ttcagtatcg cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaacg cagcatatgc
    12661 aaaaggctgg ataaagtgtt acccctctat ccagctcatc cataaatacc tgatggttgt
    12721 gctaactcat caccatcagg cattactgtg caggtggaat atccgtcact tcaggatgta
    12781 cgtcatccgt ggcgacagag gtggcggcag cagcagtcga tggggccatg atttgatccc
    12841 aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc agcagtatta
    12901 acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc accgtcaggc
    12961 tgttaaggaa atcctgccgc agtttctgat actgctccgg cgcgatatcg acgacctgat
    13021 tttgcaacga acgcatccgc tgacccatca gaatatcggt atcagcccgc gcataggcgg
    13081 caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac tcttcttccg
    13141 gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg ttgctgttca
    13201 gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac agcacacggc
    13261 agtcaaatcc aagaccgatg tctttgctgt tactggcgct taacgcttgc tgaacatgcc
    13321 agaacagcgc ctcgcgggcc aggtccgcac gccagtagcg cagcagtgcg gctgattcac
    13381 gaatcggctg ccacggcgta tcccacatga tagataaccg gtcctgacgc accgcgtcag
    13441 tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga gctggcgttt
    13501 cacgtttgcc tttcagttcg ccaaacgttt tgtttatttg gtcgacaacc gagcgcgcat
    13561 ccacgtttcc caccaccagt agcgtcattg catccggggt gtaccatttc tgatagaaat
    13621 ctttaatttt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg tgacctaaca
    13681 aggttgaccc tttcagacga tagcgccacc agccctcttt agtatcggca ggccaggttg
    13741 ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggtgtgatg gtcaatttgc
    13801 cagtggcatt tgccagataa gagagcgctt ctttcagcaa atcgttacgg ttattgggca
    13861 aactcagatt aaacagcgtg gtgtcataag agacaattac cggcggcatc gggcgtttag
    13921 ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca ccgctttgcg
    13981 ttagcgcaat acgagggatg gcgtgactgt aaccgctctg ttgtgtactt tcggcgagcg
    14041 aaccggtatt aaccagcagg cgaatttcaa cacgatcgct gggacgctgg ggggtggtca
    14101 gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga tcaggctgga
    14161 gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc
    14221 gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa aaaactgccc
    14281 gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa gaacatgaaa
    14341 atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc aggcacaggg
    14401 gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag tgatgagatt
    14461 aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca tcgtccagtt
    14521 ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg ccgttaccga
    14581 ccaggttagt cagcgcacga gcttctgaca taaagcggtc gataccgagg atcagcgcca
    14641 gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg ataaagccac
    14701 tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac agcgtgattt
    14761 ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc gccattgtca
    14821 ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc agccccacca
    14881 ccgatttgcg gcagccgagt ttctccatct tgtcgagcat acgcggcagc gccgactcgg
    14941 aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata aatttgaaga
    15001 tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac aggatacagg
    15061 taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg ccgtatttac
    15121 cgatggtaaa cgccattgcc ccgaacgcac caataggcgc cagacgcatg atcatattga
    15181 tgatgccgaa gatgacctgc gagaaacttt cgatgacgtt aaaaatcagt tggcctttgc
    15241 tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc tgcagaatgt
    15301 taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg aaggcgacaa
    15361 tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca agcgttgccg
    15421 gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga ccaataatca
    15481 gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta cgaccgaccg
    15541 ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg acaggagcga
    15601 tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc tcgcctattt
    15661 caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc tgaaagtaaa
    15721 ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag gtcttgtaag
    15781 gttatggggt acctgcggcc ttaaaataac acccagacaa catcacagaa atgtacctgg
    15841 atcataattg aaacaaaaag gttaaaaagt ttgagctggc tcgcacaaac cagcactttt
    15901 taaagttttg taatcagttt ggggtagcta cttttcttcc aggtaactct cttcgaagat
    15961 ttcaataggg agtgggcgag caaaaaggaa gccctgggca ataccaacgc ccgcttttgc
    16021 cagccagtcg cgttgtgctt cagtctccac gccttcggca atcatttgta agttcaggct
    16081 ctgcgccagc atgataattg cagcaatcat gctgctatct tccggcaagc cttcaacaaa
    16141 cattttgtcg attttcagta cgtcgattgg caacgatttc atatgctgca gctgacgcag
    16201 ccctgcgtag cccatgccga aatcatccag cgccacccga actccggcat tgcgcagcgg
    16261 acggaggatt gccaccgcag catgagggtc gtcaatacgt cggctttctg tcacttccag
    16321 aatcagtgtt cccggctgaa tgcgatagcg ggttaacagt tccagcatat ccgccaccat
    16381 atttgggtgc atcagttgca gcgcagagag gtttaccgac aagggcagca taatgccgcg
    16441 ctcttgccag gctgcaagca atcgacagga ctcttccagc acccagtgac cgacggtaac
    16501 catcagccca cagcactcaa tgcgatcgat taagccatcc ggcaggtccc aactgccatc
    16561 cggttgctgg atacgcagta acacttccgc actgaccagt tttccgctgg tcatctcgac
    16621 ctgtggctgt aaccaaatag caaactgatg attttccagt gcattaagga tatcgctctc
    16681 ttccgtcaac cgcttctggg cggcttccat ctgctgcgga tcaaagaact gaatctgatt
    16741 cttgccttta tggcgagcgg taaatgccgc agaaatagcg cgactgtaaa gctgttcggc
    16801 ggtgagatcg ccgtagaaca tcgccacgcc aatgctacag tgcggacgga gttgaatacg
    16861 ttcaatcggc aggcgctcgc tcatgatagt gagcacttgc tgacctaagg tgattgcgtg
    16921 ccacggttcc tgtacaccgt tggcaatgac agcaaagtca taaccgctaa tctgcgcgag
    16981 gatcatacgt ggcgacagta ccgatttgag tttttccacc agcgtcagca gcagaatttc
    17041 tcgttgcgcc tctttcagca cgcccgcagt atcacgcagg gtttcacagg tgatgatcat
    17101 cagcgcggtg gtttgtttac gcgcgacaac ctgctccagc atctccatca gcaaggcttt
    17161 gttcggcaaa tccgacaccg ggaagcgcat cgcattttcg ttctgttctt cataatggcg
    17221 ctgcagcaat tgctggttga ggttgtaact gcgcaccaac ataccgattt catcgtcctg
    17281 atgcagacgc ggtaatgcca gttggtgacc aacaagctcc tgggctggga tggcgttaag
    17341 ttcgcgagca atattgcgta acggatgcaa aatcaggcgg ttaatgcacc agctgatggc
    17401 gacggtcagg ataagcgaca aaagtaagta aatggtcact aacattgaga gggtgctcat
    17461 cacgaactta tacatacgga aggaatccgc ctgtagtacc agataggcaa ttggctgcgg
    17521 gtttgccgga cgttcgagcg agtaaacgcc cagcgagatt tgaaccggta gctcaaacag
    17581 gcgagtaacc attaccggca ccgggcgctc tggaataaaa cttttacgca gcgcctggaa
    17641 ctggttaggc agcactacat cggcacggct gaccacgcct gccggtttaa tgctggcaag
    17701 aatagcttcc gcttcgggaa tatcgccttt caaaatagct gaagataagg gttcgcggac
    17761 agagcgagca atgctttcca gttgcgtagc cgtgttatag cgattctgtt ggaccagatg
    17821 gaacagcaaa acggtgcaaa aaataaaaac gaacaccagg acaacggctg ccaccattgc
    17881 catctgcttg attgttaacg agcgacttac gcgcaaattg tctccccccg gaaatcccag
    17941 attgccgccc gagtataccc gatcgcagcg gcattaagag aggcgctatc tgaaaactta
    18001 ccagtcggcg taaggtatca gcggctgcgg cggtaaatcc atgtcaccct gccatccggc
    18061 ggcggaataa cgtacgtaga gcaggaaatg gctgggtgcg taatctttcg cctgctggat
    18121 atcaattgcc gtgccaacaa accagttgga agtaacacgt cgttcaagta atgcccgcgc
    18181 cgtgtagccg aagccctgac tgctgccgcc atcgttggat tgccgcgcag cttcttcctg
    18241 ccagtcggtc gggatcagat tcatcagcgg ataacgcggc atggttttgg tgcgtgaatg
    18301 cgaccacgag ccagacgccc ccagctccca cgaccagttt tccgtgcgct cccgccacat
    18361 caccggtatg gcaaacgaca ggtattcctg cggactgtag taaccgccct gaccgagtga
    18421 gtagccactc agatctttgt cgtaatgcca gatcatgttg ttcaggccga ttgtgacgcg
    18481 gcgattgttc tggttaatga ccttatagta atagcccgtc atccagcgca cgcgccagtt
    18541 atcttcgaca tttttaccgg ttaactggtc gccactaagc gatgcccaga cgccgtttgc
    18601 ttcaccttta tcgtagctca gacttagccc cacaccgtcg gcacgtacgc caccccattt
    18661 tttcccggta ttgctcgggg agtctttttg cccaccaaag gccagcaaag aactggagat
    18721 gggccgacgg tgggcgttaa cggtgtaacc cagcggcccg atatcatcgc tgtaactgat
    18781 gccgccgacc acatccacca cgttgaagcc catcggcgtg gtaccgatat cccagctcca
    18841 gacgtcattt cgccagccga ccgccacgct ggcaccggaa tccgactggc tgcggttgcc
    18901 gctacagtcc tgtaatgtac aggtgcccca gttgtcatcc catttgccat cggcattagt
    18961 ggagaaactg ccgacgttca tattgacgaa atcactgcga aagaacatcc gcccgtcaga
    19021 atacggcgca tccacctgca acatggtagt gtgcgctttc agatcggagt aaccaccggt
    19081 gccgctcgaa ccccagtaat cgtgctcgag ggtgacgtta agatcctgct ggcgatagag
    19141 gtccgccgca tcgctgcgca cgccgcgttt cagccagtca tctttctcgt cgttacgggt
    19201 cagtcgggtg aatgtgtcgt tatcctgcgg acgcgtcgta gtcacaccgg atgcgaccat
    19261 ggcgtctttg taggtttcca gcgcctgctt tggatcgccc gcctgcgctt caaacttcgc
    19321 accgtcgcgc agcaccatcg cgctttccat cgacggtggc tgagattttg cctgcgggat
    19381 caacttatta aacgtctgct gcgccgctgc ggtatctcca agctgcgcct gcgccagcgc
    19441 cacgcgccgc tgtgtgttca gcgaggcgtt atcggtagcg ggcagtttcg ccagctggct
    19501 acgtgccgcc gctgtgtcac cggcagcaat atccacttcc gtcagcccca gaatggcatc
    19561 ggcgttagtt ggctccagcg tcaggacatt ctgatatgca gcgcgggcgg cggtgtaatc
    19621 acgtcgctgc tgcgcccagt cagccagcgt gaggtcaata cgcgtggaag gtggttgctg
    19681 acgcagcatc gcttccgctt cagcttcttt gccgctttct cgcaggcgat tagcggtttc
    19741 cagcacctga tcgctttgca gtcgattaac cagctcctga atattgctgt tccactgcgc
    19801 gcgcggcagg ctgttgatat gcgccagcgc cgctctgtcc tggtcatgac cagagaggta
    19861 cagcccgtaa gcgtaaacct gctccgggtc gttcggcttc tgctgcgcca gattgcgcat
    19921 taacgtatcg gcctggctgc gttgtccggc ctgccagaga tcctgcgaaa gtcggtaagt
    19981 aatccatacg ctgccggggt ccagcgccag tcgttgccgc tgaagtgctg ccgcctgcgc
    20041 ccatttgccc tggttttcca gtgcctctgc ctgctgtgcc agacggtcgt tttgcaggct
    20101 gcgttcgata tcatcaatgc tacgccgctg actggcagag agcgaggcga taaacgcttc
    20161 agctttttct ggcgattgct ggcggtaaat atttgccagc ccgcgcacgg cgttagtgtt
    20221 gccgctgtcc atacgcaagg tctgctgata ataacgttcg gcggcgggat aatcttttcg
    20281 cgccatcgcc acatcgccca gccccagcac tgcataactg tcggtgttat cgacattacg
    20341 cgcctgctgg aacaggcgtt ctgcccggtc aggattattg gctttcagcg cagcatcgcc
    20401 ctgctggatc gccagccagt agcggtttac tttcagcaga ctgttccatt tgtcgttgtt
    20461 gctgctgtgc gggtccagtg cgagggcttt ttccagattc gccactgcat tggcgcgatc
    20521 gcctttctga gaatacgcct ggcccagcgc ccccagagct tcactgtctt tcgggttcgc
    20581 ccgcaccgcc tgttgtagtt cgggaatggc tttacccgcc ataccagagt ccaccgccgc
    20641 taaaccttgc gcacgagcgc ggaaagcagg atcggccagc tgtttttgct gttctgccag
    20701 ttgcgattgc gcagccgcca cgctatcgcc atcactaaag atcgagagat attttttcag
    20761 cgccgacaca ctggcatcac tgacgggcat gtctttaatc tgcccgtacc agattttaga
    20821 ggccccttcg cgcccggcgt tcgattttgc catctgttcc aggacggcaa aaccttcgtc
    20881 acggcgatcg ctactaaaca gcaatagcgc cagattgttt tgcaggcccg tattgcccgg
    20941 tgcatccgca ttgatgcgtt ttaactgatt aatcgcttcg ccacggcgag ccggaatttt
    21001 cgccaccgta ctccagtact cgacagcaat gtcaccttcc ggcggcgcac cgttgaacag
    21061 tttgttgtaa ctcgccacag cttcttctgc atgaccggtc gtcgcctgca atcgtgcctg
    21121 ttgcagtgcc tgacgaccat ccggcgtgga aagtagcatc gtagtccgcg acgatttata
    21181 cgcatttgaa ctcggcgcta actgcgacag ccgatcgagc tgtttttgcg cgccatcaat
    21241 atcgccctga cgtaacaaag aacggaaacg ggcggcaacg acgtccgggt tattcggatc
    21301 aataagttcc agccgatata acgactgttg caccagatct tcacgatggg tcgcttcgcc
    21361 taaccgaact tgctccagca actgttgctg agcggttggt gctgcctcga ccatcggcat
    21421 gacggccaga ccgagggaaa gcgtgaatat gtttagtgtg aatttgcgca ttcctggccc
    21481 cagtcaggta ataactcacc ttttgtcgag aagcggaaac ggtgttgatc ccagccttgt
    21541 ccaaacaggg tcagcacata gttgtaatag gcatcgctgc cgggaaagtt atcggccacg
    21601 cgctggcgct gaacggcctg cgcatcgcgg ttttgtaaaa agggcagcat ggcggcagaa
    21661 aaaccgaccg gtcctttacc ctgcgctttc cccgtagcca catccacttt ttccggcgga
    21721 taaccgtttt tctcagtgaa tgtcgccatc ggtttaaacc ggttgagcat ccgcgctttt
    21781 tgcggatcgc tgtcaggcat catgcctacc cacatgtaaa cgcggatagc gtcgtagctg
    21841 ctgatcaatg ttttttcggc ttttagctgc cagcctttgt ctttctcata gcgcacccag
    21901 tctggcgaaa aacctttcgg ggcggtttcc agcaataaac gttgattggt ttcgcgcagc
    21961 gtggtccacg gcgcgccaaa gcgggtgaaa tactgcgcca gcgtcggcgg caggtagctg
    22021 gggttaaaac gccagctgtt atcctcagca aaacccactt tgcccggtaa caacatggaa
    22081 cccagcccag gcaccgtcac cacttcctcc cgcgcgatac gttttagcaa cgcgctgccg
    22141 atgtcggtat aacgctgctc tttccacaaa cgccccgcct ccagcaacga ccaggccatc
    22201 cagatatcac catcggaggc cgaattgctg tccagcactt cccacttact gttctctttc
    22261 ttgccccaca gccaggcggg caaacgttct tttaaagaac cctgagcgag attgttctgc
    22321 gtccagtcga gaatattatc gaaagctgca cggtcgttag ccgccagggc aaagaacatg
    22381 ccgtaacttt gcccttcgga ggtggtgatt ttgcgcgcgt cgctggggtc gatgacgcgc
    22441 ccttcctgac tgatgtaatc ctttttaaac tgctcccagg caggccaggt acaggctgcc
    22501 tgaacactaa aggcagccag cagcagcatc gtcacgattc cactacgcaa cacattcatc
    22561 ttcaattact cgttatccgg gttaagacga cgacgactaa taattcgcag cagacgccac
    22621 agtacccatg ccagcaatat cacactgata gccgccagca ccgccagcag aatcggatgg
    22681 tttgccagcg cataccacac gcgctcgaac cacggcagat gacctacgta ataaacgtcg
    22741 ccaacacgta ggctgttgat accggactcg cggatcaccg cgaccgaacc gaacatggtg
    22801 gcgcgtttgc cgctatcgtt caccgcatcg ttaagcattt cataaccgcg tgggctatct
    22861 gccaacagcg caatcacgct gcgctggtcg ttatacggcg actggaagcc aatcaccgcc
    22921 gccatcgcac cggaagaggt cagcgttgac cgggtttctg ccgcgcgatc gctctcgtcc
    22981 ggcacaatgc cggggaacgg ggtctggcgc atcggtgttt tcacccagct ttcggtcgcc
    23041 tgcaccaata ggtcgatctg cttatcgtct ttcagtttgt ccgggatacc accgatgatc
    23101 atgatgtcgg catctttgcc ctgaatggtg ctgccatcat cggtcaccgt cagattaatc
    23161 gccgggaagc ccgtctgtgc gccgataaaa ccaacagtat tcagcaacgt ttccatctgt
    23221 gcttcgttag gcgctttcgg catcacggtg atggtttgcg acagatccgc catccggctg
    23281 aatgggaagc ccgcgttagc aaaggcgcgt agatccggca tcgggatgaa gtggtaatac
    23341 ttcgagaagt cgatggtgga gtcgtcacca atcaccacat gattctgcac cggctggaag
    23401 gtaatacagt tatccaccga accgcccggc atcgggttca tatactcaaa gtcgaagcgc
    23461 agctggttgg tcgcgcccag tttcagcgcc ggaatagaga catctgtttt gccatccagc
    23521 aaaccttgta ataccggaat ccgcagcagc aggcggttcg cctcctgttt gctgctcaga
    23581 ttgaaggatt gcaggaactg gttattcagg ctgatatcca tccgcgaact gtctttcacc
    23641 ggcggcatgg tgtagcggta attaatatcc atatcaatgc cggtactgcg catcaggtag
    23701 agatccggcg gcaggtttag cgaaacgtta atcgctgctg gctcaagacc gctggattgt
    23761 aactgttctt cataggtttt cagttcgcca aatgtgaccg gacgatcggt acgtacccag
    23821 ttcggcgcat cgtacggctt acgcggtagc agcggtttca cttcattcac taccacgctt
    23881 tcaccacgga acagaatgtt accctgagcg atacctttcg ctgcctgcaa caggtctttg
    23941 tcgtcacgac caaacaccac cagcagtttg acgtaaggat tctgcggatg gttaatcatc
    24001 tcaatcacag gggcttttac cgccggatga tcgcgcagga agtccggccg tttgtcgttg
    24061 gttgcaaaga caatggcatt acgatccggc agttggttat agagcaccgg gaagttctgc
    24121 ccacgccagc cagaacgcga accaaaccac gaggcgacaa tggcagaggc ttgttgcagc
    24181 ccaacatccg gcgcacccgc aaagaccatc ggcaaggtgt tagtacggtt atcgcgcggg
    24241 tcaaagaatg gcaccgggaa gtgtgacagg tcattcttca cattcagggt ctgataggtc
    24301 agatccagtc cactgctccg cccaacatcc agccaaagcg tggtgctggc cgggttttcg
    24361 cacacgtcct gataatggcc gacaaactcc agccgcacac ggttgaagtc ggtaataaac
    24421 agtgggttaa tgggcatttg cgccagcgtt tttttaccca actgttcttt ggtcactggc
    24481 agcacgccca tcagctcatc attgagataa acctttaact gcgactggac aggcagtaac
    24541 gatggcgatg gcgtgtattc gaggttgagc atcgccttcg tcaccacttc atcgctgcgc
    24601 atgccaaact caatgctgcc gttcgggtta atgccacgta gcaccatgct gcccggcggc
    24661 ggcgcaattt gcgcaaaggt cagcttcaca tcacgcgaag gaccgttctg cgccacgact
    24721 ggcgcatcag cgccctgcac gcccggcatc acttgcccca cctgcggatt ttgttccgtc
    24781 tgggcggcta cagctggctc agcattgatc agtggttgcg ttgctggcgt cgcctgcgtc
    24841 atgaaagagg ggaacgcact catccccata gccactgcac aaatccagaa tagttttctt
    24901 ttcatcgcgt tatcatcatt gttgagccaa agcctgatcc gatggttgtg ccgtttcgct
    24961 ccgctccggg cggcgcggaa taaacgatac aacccaggaa accagagaag tcagcacacg
    25021 gaatatgccc ttcaccgaag aaggcgcaaa ctccgccaga tggcggtagc cacggaagcc
    25081 gagcttcaga atatccagca gactttccag cggcttatct tccgggtagc tgtcctgcca
    25141 gagcgcccat gtatccgcac gggcaaacgt acactgcaca aaatcgatat gttgctgggt
    25201 ggtgagcggc attaatttca gcccaacttc attacccatc acgcgcgcca cctgggtcgg
    25261 gaagacgtat tcctgctgac cgcgtttaag caacagattc actttctgcc cttccagaat
    25321 ctgcgcctga ccgttgatct tgatccccaa accaccgtcg gagaaatcct gaacggtaca
    25381 cgagaagagg tgaccatctt cgcgggcaat tgccgcgggc atcgtcatct ccacgcggtg
    25441 cgatcggcgt acctgtttgc tttctaccga tactgcaact gcgccgccaa gaacaatcag
    25501 gttgtagaat acccacacca tactgacgac cacggtgagc atctcggttg gcgggccata
    25561 gaagtagcgc cagatgccta ccgcaacgcc caccaggttg agcaggacaa ggaagatgta
    25621 gggccgcgag atcacccagt cgacgtactc ttcttccacc agtccacctt tggcggtgac
    25681 gttaaatttg cctttgtgcg ggttaatcag cgccaccagc gtcggtggtg cgatatacca
    25741 cgccagcacc gtttcgtaga tttcactcca gaaagagtgg cgatatttgc cctggatctt
    25801 ggagttggtc aggctggcat ggatcatatg cggcagcacg aacagggcga tcatcaacgc
    25861 tggcgcatag atgatgtagg catgaagcag caggaacgcc agcggcgcag tcaggaagat
    25921 cagtcgtgga atgcccgaca agaagtggaa catggcgttg acgtagcaca gccgctgggc
    25981 gaatttcagc cctttaccgg taagcgggtt atcgagacgg aagatttgca ccatcccgcg
    26041 tgcccagcga atacgctgac cgatatgcgc cgacagactt tcggtcgcca gccccgccgc
    26101 ctgcggaata cgcatatatg cagaggtgta gccacgacgg tgcaggcgca gagacgtgtg
    26161 cgcatcttcg gttaccgttt cgacagcaat accgccaatt tcatccaatg gtttgcgacg
    26221 aattactgca caggaaccgc agaagaaagt ggcgtcccac atatcgttgc catcctgcac
    26281 cagaccatag aacagcgtgc cttcgttcgg cgttttacga aaacgcccca ggttgcgttc
    26341 aaacgggtcc ggcgagaaga agtggtgcgg cgtctgcatc atcgccagct gtttctcttt
    26401 caggaaccag cccatggtca tttgcaggaa cgatcgcgtt ggtacgtggt cgcagtcgaa
    26461 aatcgacacg aactcgcctt tggcatattt cagcgcattg ttgatgttgc ccgctttcgc
    26521 atgttcatga gtggtacggg cgatatattt cacccccacg ttttgcgcaa actggcgaaa
    26581 ctcttcccta ccgccgtcat cgaggatcca gatgttcagc ttgtctttcg gccagtcgat
    26641 acccagcgag gcgtaaatag tatttttcac cacgttgaga tcttcgttgt aagtcgggac
    26701 aaagatatcc accgacggcc acagcgacat atctttcggc aatggcaccg gctgacgatt
    26761 cagcggccat actacctgga agtagccgag caccagcaca atccacgcgt acgtttcagc
    26821 gaagagcaga ataagcccgc acaccaggct gaccggatcg tcccagttca gcgtagaggt
    26881 gtaacgccac cagatataac ggcaagaaac ggtcagcgac agcacaatca acattagcgc
    26941 cgagaagcgc cccggcatcc gccgtacgat cagcgctacc ccccacagca gcatcaggaa
    27001 gataaactgc gccagcgggt taaacggctg agtaacgcag attaacgcca gaatcagcga
    27061 gaaggtgacg atgataccga ggatcaaccg ccgcgccccg gcactcaaat gaccgagctc
    27121 ttttttctca tccagatgct gtgttttatg gctaacgcgc tcaggcagct cgttcatcca
    27181 ttgatggtaa cgtccacgaa tattttgcag acctgaaaat gcccgcctgc gcggtttcgg
    27241 cgtttctttg cgcgatgcac cgatcaataa ccagcatgtt tgaatgagat aacggaccgg
    27301 gtccagcgga cgcggacgcg aggcgttgat atgcggatac aggtttttat gttctgcgcg
    27361 aatacgctgc cagcgcgggt gctccagcgg aataaaaatc caggccagga tcatccagaa
    27421 acagccgagc gtcgcgctga aagccgacgc accgtggcga cgataatcgc gataacgccc
    27481 gataagccgc gcgttgaccg gcgggataag caaccaccgg gtcaggatac tcatgatgca
    27541 ctcccgactg gcgttttcag cccggaatag ttcaacaggc accagttcgc cagcgtcagt
    27601 atctcttctg ccgccagcgc atcactgcgg tattcaccga ctggttgctt agccgccagg
    27661 cattcagcca tcgcttcatc acgatgaatg agcatcggca gcaatcggcg ctggctttgc
    27721 aaccaaagct ggtaaatatc gtcctgaacc tgactgccaa tacggaagtt attaatcaaa
    27781 atatgtgcgc catccggcag cgcttgctga tgcaggcgga tatggcagtt ggcatccaca
    27841 ttgacgatag ccagcgagtg atcgcacaaa ctcagcagct ggtgggttat ctgcgaggca
    27901 tcacgcggta agtcgattaa aatccactgg taacgcccgc tggcttttag ttgctgtaag
    27961 ccggagcaaa tatcgctcag ccgggtttgc cagtgctgtg gattttcttg ttcttcaatg
    28021 gataactgac caaaaggcag caaatcgagc tgcgaggtgt agcgcaaccc agcgtcacgc
    28081 cagtcctggc catccagcat cgctctggcc cagccctgac ggtgggtaaa atcaacatta
    28141 aatgacaggc gcaacaagtt gtccgggcag gcatcgacca ccagaacatt ttctcccaac
    28201 atttgtaatg accaggctaa tgcggcggtg atggttgttg tccccacgcc tccccgcacc
    28261 ccctgcaatc ccagtacggc cattcaccaa tcccctactt ttgttgcgca aactctgcca
    28321 gcaacggcca gcgttttaat gccgcggcca actgttcgcg ttgggaaata tcggcataat
    28381 caatatcagg cagtgaaaat gcctgcttta acgccacaat atcattctgg aagatatagc
    28441 ctatcgcggg atcaggcaga gtatctggtt cattgttatt cattttgctt gatccctatg
    28501 aaataaccgt accgttcatg agacaattct aaccgtgagc atttcgccgg tttattgttt
    28561 acggcgagac gggaatttgc tcactttttg actttccgtt acgcaacaga aaaagttaaa
    28621 aacgaaaaga ctaaaatcac acgtcttgtg accttctaaa ttgattcaca tgctaaatct
    28681 gataagtttt aatttcaatg gtaggtttat ttcttagctt tcgctagtaa actgataatc
    28741 agacaaaatg gtgacatgag ggacattgtg gaccctgtat tctctatcgg tatctcatca
    28801 ttatgggatg agctgcgaca tatgccagca ggcggcgtct ggtggtttaa cgtcgatcgc
    28861 catgaagatg ctatcagtct ggcgaatcaa acaattgcat cccaggctga aaccgcacac
    28921 gtcgcggtca ttagcatgga cagcgatccg gcgaaaatct ttcaattaga tgattctcaa
    28981 gggccggaaa aaataaaatt attttcaatg ctaaatcatg aaaaaggtct atactatttg
    29041 acccgtgatt tgcagtgttc tattgatccc cataattacc tttttattct tgtttgcgca
    29101 aataatgcat ggcaaaacat tcctgccgag cggcttcgct catggttgga taaaatgaat
    29161 aaatggagca ggttaaacca ttgttcgctt ttggtaatta atcccggaaa taataacgat
    29221 aaacaatttt cattgttgct tgaggaatac cgttcacttt ttggtcttgc cagtttgcgt
    29281 tttcagggtg accaacattt gctggatatt gccttctggt gcaacgaaaa aggggtcagc
    29341 gcccgtcagc agcttagcgt tcagcaacaa aatggtatct ggacattagt tcaaagcgaa
    29401 gaggcggaga tccaaccacg cagcgacgaa aaacgcattc tgagtaatgt tgctgtactg
    29461 gaaggtgcgc cgccgctatc ggaacactgg caactgttca acaataacga agtcctgttc
    29521 aatgaagccc gtaccgctca ggcggcgacg gtggtctttt ctttacagca aaatgcgcaa
    29581 atcgagccac tggcccgcag cattcatacc ctgcgtcgcc agcgcggtag tgcgatgaaa
    29641 atcctcgtgc gggaaaatac cgctagcctg cgcgccaccg atgaacgttt gttattggcc
    29701 tgcggtgcaa atatggttat tccgtggaat gcgccactct cccgttgtct gacgatgatc
    29761 gaaagcgtgc aagggcagaa gtttagtcgc tatgtgccgg aagatatcac taccttgctg
    29821 tcaatgaccc agccgctcaa actgcgtggt ttccagaagt gggatgtgtt ctgtaatgcc
    29881 gtcaacaaca tgatgaataa ccctctatta cctgcccacg gtaaaggcgt tctggttgcc
    29941 ctacgtccgg taccgggtat ccgcgttgaa caagccctga cgctgtgtcg ccctaaccgt
    30001 accggcgata tcatgaccat tggcggtaat cggctggtgc tgtttctctc attctgtcgg
    30061 attaacgatc tggataccgc gttgaatcat attttcccat tgcctactgg cgacattttc
    30121 tcaaaccgta tggtctggtt tgaagatgat caaatcagtg ccgagctagt gcagatgcgc
    30181 ttgcttgccc cagaacaatg gggcatgccg ctgcctttaa cgcaaagttc taaaccggtc
    30241 atcaatgccg agcacgatgg tcgccactgg cgacgaatac cagaacccat gcgactgtta
    30301 gatgatgctg tggagcgctc atcatgatga ccatcagcga tatcattgaa attattgtcg
    30361 tttgcgcact gatatttttc ccgctgggct atctggcgcg gcactctttg cgacgcattc
    30421 gcgacacctt acgtttgttc tttgctaaac ctcgttatgt taaaccggcc gggacgttac
    30481 gccgcacgga aaaagccagg gcaaccaaaa aatgactcaa tttacgcaaa ataccgccat
    30541 gccttcttcc ctctggcaat actggcgcgg cctttccggc tggaacttct attttctggt
    30601 taagttcggc ctgttgtggg cgggatatct taacttccat ccgctcctca atttggtgtt
    30661 tgccgcgttt ctgctgatgc ccattccgcg ctacagcctg catcgcttgc gccactggat
    30721 tgccctgccg atcggctttg ctttgttctg gcatgacacc tggttgcctg gcccggaaag
    30781 cataatgagc cagggttcgc aggtggcggg gttcagtacc gattatttaa tcgaccttgt
    30841 cacacgcttt attaactggc agatgattgg ggccattttt gttttattag tggcctggtt
    30901 attcctgtca caatggattc gcattaccgt ttttgtggtt gccatactgc tatggctgaa
    30961 cgtacttacc ctggcgggac caagtttctc cttgtggcca gccggacaac cgacgaccac
    31021 tgtaacaacg acgggtggta acgcagcggc aaccgttgcg gcgacgggtg gcgcaccggt
    31081 agtgagtgat atgcccgcac aaactgcacc gccaacaacg gcgaacctta acgcctggct
    31141 gaataatttc tataacgcgg aggcaaaacg taaatcgacc ttcccgtctt cgctgcccgc
    31201 tgatgctcag ccatttgaac tactggtgat taacatctgt tcgctttcct ggtcggatat
    31261 agaagccgcc gggttgatgt cgcatccact gtggtcgcat ttcgatattg agttcaagaa
    31321 ctttaactcc gccacctcct acagtggccc ggcggcgatc cgtttactgc gcgccagctg
    31381 cgggcagact tcgcacacta atctgtatca accggcaaat aacgactgct atctgtttga
    31441 taacctttcg aaactgggct ttacccagca cctgatgatg gggcataacg gccagttcgg
    31501 cggttttttg aaagaagttc gcgaaaatgg cggcatgcag actgaattga tggatcaaac
    31561 aaatctgccg gttattttgc tgggctttga tggttcgccg gtttatgacg ataccgccgt
    31621 gcttaaccgc tggctggacg ttaccgaaaa agataaaaat agccgtagtg ccacgttcta
    31681 caacacgctt ccactgcatg acggcaacca ttatccgggg gtcagcaaaa cagcggatta
    31741 caaagcgcgg gcgcagaaat tctttgatga actggacgcc ttctttactg aactggagaa
    31801 atcgggtcgt aaagtgatgg tggtcgtggt gccggaacac ggcggcgcgc tgaagggcga
    31861 cagaatgcag gtatctggcc tacgtgatat ccctagcccg tctatcacag acgtccccgt
    31921 tggggtgaaa ttcttcggca tgaaggcacc acatcagggg gcaccgattg tcattgacca
    31981 accgagcagc ttcctggcta tctccgatct ggtggttcgc gttcttgatg gcaagatttt
    32041 caccgaagac aatgttgact ggaaaaaact caccagtggg ttgccacaaa cagcaccggt
    32101 ctccgagaac tcaaatgcag tagttattca ataccaggat aaaccgtacg ttcgcctgaa
    32161 cggcggcgac tgggtgcctt acccgcagta agcacgaaaa aggccgcaga gtttaacctc
    32221 tgcggcctga tccgggcgca aattgccatt acggcagcct gacgcccgca tgacacgtta
    32281 cttccgctta ttcagccagt tcacgatcat actggcaaga atgccagcaa tgaccggagc
    32341 cgctaaatca tgccagaagg ccatgcccag ctctgcgagc gtcatatagc cgcctgtgtt
    32401 gtaatgacaa cgtttcgcgg ctattcttga gttgtttggg ttcaagatta gcccccgtta
    32461 tgttgtcagg tgcatacctg caacgtgcgg gggttttctc tctccagcaa ccaatgccac
    32521 cagggataaa gcccccgcaa cattgcgcct caccggaacc tttccggctt gccgctgatt
    32581 ctacgactgt ttttctgttc cgaaatccat cacttactca ctctgtgctg cgcattccgc
    32641 agaatattat ccgcttgcaa ccgttgcatt tttcttacga aaaaggccgc agagtttcac
    32701 ccctgcggcc tggtccgggc gcaaattgcc attacggcag cctgacgccc gcatgacacg
    32761 ttacttccgc ttgttcagcc agttcacgat catactggca agaatgccag caatgaccgg
    32821 agccgctaaa tcatgccaga aggccatgcc cagttctgcg agcgtcatat agccgcctgt
    32881 gttgtaatga caacgtttcg cggctattct tgagttgttc aggttcaaga atggcccccg
    32941 tgatgttgtc aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc
    33001 caccagggat aaagcccccg caacattgcg cctcaccgga acccttccgg cttgacgctg
    33061 attctacgac tgtttttctg ttccggaatc cgtcacgtct tcactctgtg ctgcgcattc
    33121 cgcagaatat tatccgcctg caaccgttgc atttttctta cgaaaaaggc cgcagagttt
    33181 cacccctgcg gcctgatccg ggcgcaaatt gccattacgg cagcctgacg cccgcatggc
    33241 acgttacttc cgcttgttca gccagttcac gatcatactg gcaagaatac cagcaatgac
    33301 cggagccgct aaatcatgcc agaaggccat gcccagttct gcgagcgtca tatagccgcc
    33361 tgtattgtaa tgacaacgtt tcgcggctat tcttgtattg tttggatgca agaatggccc
    33421 ccgtggtgtt gtcaggtgca tacctgcaac gtgcgggggt tttctctctc cagcaaccaa
    33481 tgccaccagg gattaagccc ccgcaacatt gcgcctcacc ggaatccttc cggcttgacg
    33541 ctgattctac gactgttttt ctgttccgga atccgtctca tcttcactct gtgctgcgca
    33601 ttccgcagaa tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa ggccgcagag
    33661 tttcactcct gcggcctggt ccgggcgcaa attgccatta cggcagcctg acgcccacat
    33721 gacacgctac ttccgcttgt tcagccagtt cacgatcata ctggcaagaa tgccagcaat
    33781 gaccggagcc gctaaatcat gccagaaggc catgcccagc tctgcgagcg tcatatagcc
    33841 gcctgtgttg taatgacaac gtttcgcggc tattcttgag tggtctaggt tcaagattag
    33901 cccccgttat gttgtcaggt atatacctgc aacgtgcggg ggttttctct ctccagcaac
    33961 caatgccacc agggataaag cccccgcaac attgcgcctc accggaaccc ttccggcttg
    34021 ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc attgcgcgat
    34081 gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg ctgactccag
    34141 actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc cccagtgaat
    34201 ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc gcaagagaaa
    34261 ctgggtttat tcatttttgc gagaccgact tctttctgga caggacttta tgcagcacaa
    34321 cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg gctgggtttt
    34381 attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac aaattggctt
    34441 gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca cctgggtagt
    34501 gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact acaccgatat
    34561 tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct actttttgat
    34621 gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg cctcgtacct
    34681 gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata aagtcgctat
    34741 tttcgccgta ctggtggcga ttgcgtctgg tggtgaacga ttactgttta agatttccgg
    34801 gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga tgatcccgca
    34861 ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc gcgatgtcct
    34921 gcttacaatt ccattttgct tcttttctgc ggtatttatt caggtactta acccaatgaa
    34981 tattgcctat cgtaaacggg aagcagataa agtactggca acccggctcg cgctgcgtac
    35041 ccaccgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct tttcgtttac
    35101 cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct cggcactggc
    35161 gctggccgcg caggtgatcc ctggacatat cattcatatc acctctacgg tgctgaatat
    35221 ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg ctattaaagg
    35281 cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact cacgcatgct
    35341 gactctggcg atctgcgctt ttatcgtcat tacattgacg atttgggttt cgtttcgtgt
    35401 atcggtgctg gtgttctttc agttgggaag cccgttatat ggcattgtgt cgtgcctcat
    35461 tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat ttaaagcctg
    35521 gctaattctg ctttacggca ttttgctatg cttgtcgcca ctgttgaagc tgattgagta
    35581 aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat cctgatcaac
    35641 agcaaaacaa gcgaccagtt gaccgccgta gtcttttagc tgcggctgca actgggtgca
    35701 ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggag gattcagcgg
    35761 gcttggcagt tcaccggtga gtttgatgcg ttcacgacga tcgtccgggt tcaggcgtgg
    35821 cgtcgcggag agcagcgcct gggtgtacgg atggcgcggg ttattgaaga tttggtcctt
    35881 cgttcctttc tccacgcagc ggcccaggta catcaccatc acttcatcag caatgtgctc
    35941 caccaccgac aggtcgtggg agataaagac ataagaaagc cccaactcct gctgcaaatc
    36001 catcatcaga ttcagcacct gcgcacgcac tgagacgtcc agcgcagaaa ccggttcatc
    36061 ggcaatcacc acgtccgggt cgagcatcag accacgggca atagcgatac gctgacgttg
    36121 tccgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc cgactttcgc
    36181 catcatcgac agggcttttt cccgacgctg ttctttgctg aagctggtgt taataagcag
    36241 cggctcttcg agaatttgcc caactttttt acgcggattc agcgaaccgt aagggttctg
    36301 gaagacaatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt gcttgagcag
    36361 atcctgcccc tggtaataca gctcaccacc ggtgggcgtt tcgatcatcg tcagcaaccg
    36421 accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt tgccacgttc
    36481 aaggttaaac gaaacgccgt ccagcgcctt caccagacgt tccggcgcga acatgccttt
    36541 cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt gcgaggtggc
    36601 ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg gcatttggac
    36661 tgacgcccat cagcgagcat attcagcgcc ggttcttcag cgcgacatct gtcagtggca
    36721 taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttacc gggaacgaca
    36781 cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc acgcagcaat
    36841 gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcacc ggtttccacc
    36901 acctggcctg catacatcac gatgatttta tgtgccgctt ccgccaccag cgccaggtca
    36961 tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcag tagttcgatg
    37021 atttgcgcct gaatggtcac gtccagcgcg gtggtcggtt catcggcaat cagcagtttt
    37081 ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc ggaaagctga
    37141 tgcgggtaaa tatccagacg cgatgccgga tcgggaatac cgacctgatt cagcaggtca
    37201 atcgcccgct gacggcgagt gctcttatta ccgccctgat gcaccttaat cgcttccatg
    37261 atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg gaagatcatc
    37321 gccacttcgg cacccaccag gttgcggcgc tctttttctg atatacgctg caaatcctga
    37381 ccgttaaaca ccagcttttc cgccattacg cggcccggat aatcaatcag ccccataatc
    37441 gccagtgaac tgaccgactt accggaacca gactcaccca caatcccgac cacttcgccc
    37501 tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gtgcgctttc gtcgccgaaa
    37561 tgcaccgata atttatctac atttaataac gccatctcga acctcttact gcttcagttt
    37621 gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca ccgtcagcag
    37681 gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact gcaacacgtc
    37741 ggagagcatg gtgccccact ctggtgttgg cggctgcgct cccatgccga ggaagccaag
    37801 ggcggccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa tcagcggcgc
    37861 aaggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac ccgccacgcg
    37921 agacgcggtg acgtaatcgc ggttaacttc caccagcacg gcggcgcggg ttaagcgcac
    37981 atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg acgggccgaa
    38041 gattgccacc agcaccagcg ccagcagcag acttggcagc gccagcatga tatcgaccac
    38101 gcgcataatg atgttatcga ccaggccgcc aaagtaaccg gcgatcaggc cgagaataac
    38161 gcccataatc agcgataaca caactaccag acagccaacc agcagcgaca ggcgcgcacc
    38221 gtacatcagg cgcgacagca catcacggcc tacgtcatcg gtgcccagca agtgcgccat
    38281 gctgccgcct tcctgccagg ctggcggggc gagcagtgca tcgcggaact gttccgccgg
    38341 gttatagggt gcaatccagt tggcaaagat cgcgatgaac agcacgatga cgacgtaaac
    38401 cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc cagaactcct gtaacggggt
    38461 catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga tgtcccctta
    38521 cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca gcaagttgac
    38581 gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat agtcgcggcg
    38641 ttgcagcgcg tcaatcaacc agcgccccag accgggccac gagaagatgg tttcggtcag
    38701 aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacta ccggcagcat
    38761 cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gttagccctt tggcgcgcgc
    38821 ggtgcggatg taatcctcgc ccagcacttc cagcatcgag gagcgtgtca tacgcacaat
    38881 gaccgccagc ggaatagtac ccagcacaat ggcaggcaag atcatatggg cgacggcatc
    38941 gataaagttg ccgtcttcac cccagatggc ggtgtcgatt agcataaaac cggttaacgg
    39001 attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg tcaggttcca
    39061 gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca tcgagtagcc
    39121 ggtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa ccgcgtttaa ccgcagccag
    39181 cacgccgacc ggaataccga ccgcagtcgc aaaaatcatc gcgcagacgc caagttccag
    39241 cgtggcctgg aggcgcggca cgaactcttc ccaaaccggg atgcggcttt tcattgaaat
    39301 gcctagatcg ccatgcataa cgccccaaat gtaatggaga tactgctgcc acatcggttt
    39361 atctaagccg agttcagcca gcagctgcgc gtgacgctct ggggagatcc cacgttcgcc
    39421 cgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg tgagaagggt
    39481 aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact gcaacataac
    39541 ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct cacaaatcaa
    39601 ttcccgccta ggtcgggctg ggaatgaagt attgctgccg gatggcgctg cacgcagagg
    39661 cacagcgcca ccgggtatgg cttttaatta ttcgatagag acgttttcga agtgatgttt
    39721 gcctaatgga tcaaccacat agcctttaac ttctttgcgt accggttcaa acacggtgga
    39781 gtgagcgatg atcagtgccg gagcctgatc gtgcatcacc acctgcgctt gcttgtacag
    39841 ttcaacgcgt ttgttatggt cgtcggtagc acgcgccggt tgaatcagat cttcaaacgg
    39901 tttgtagcac cattttgagt agttggagcc ttgttcagag gcggcgcagc tgaacagggt
    39961 ggcgaagaag ttatccggat ccccgttatc gccagtccag cccatcatta ccgtctggtg
    40021 ctcgccatct ttcgcacgct tgaggtactc accccattcg taggtgacaa ttttggcctg
    40081 cacgccgact ttcgcccagt cagcctgaat catctccgcc atgcggcgag cgttcgggtt
    40141 atatgggcgc tgtaccggca tcgcccacag gtcgatggag aaaccttttt ccagacccgc
    40201 ttctttcagc aaggctttcg ctttttcagg atcgtaggtg taatcctgaa cgtcgtcgtt
    40261 atagccccac atggttggcg ggatcaggtt tttcgctgat acacccgcgc cctgataaac
    40321 cgctttgatg atcgcgtctt tgttcaccgc gtaggtcagt gcctggcgaa ctttcacgtc
    40381 atccagcggt tttttctgca cgttatagga gaggtagccg acgttcagcc ccggcatttc
    40441 catcaggttg atggatttat cctgcttcat gcgagcgatg tctgccgggt tcgggtacgg
    40501 catcacctgg cattcattct tctgcaattt cgcataacgc acggaagcgt caggagtaat
    40561 agagaagacc agcgtatcga tctgcggttt ggtgccccag tagccatcaa acgctttgta
    40621 gcggatacgg gagtcttttt ggtactgctg taactggaac ggaccggtgc cgattgggtt
    40681 gaggtccagt ttttccggtg taccggcttt catcatcgcg tcagcatatt cttttgacag
    40741 aatagaggcg aagtccattg ccaggtcagc gaggaacggc gcttccgggc gagtcagcac
    40801 aaactgaacg gtgttgtcgt ccaccttttt cacttcgctg atcagctccg gcaagcccat
    40861 gccttcgaag tattcgtagc tgccgccaga aactttatgg tacgggtttt gcgcgttttt
    40921 ctgacgatca aacgagaaca ccacgtcatc ggcgttcagt tcacgcgtcg gtttgaattc
    40981 tttattgtcg tgccacttca cacctttacg cagatggaag gtataggttt taccgtcttc
    41041 gctgacttcc cacttttcag cgaggcccgg gatcacttca gtggtgccga ttttaaattc
    41101 aaccaggcgg ttataaagcg gtacggaaga ggcgtcatag gtggtgccgg aggtaaacag
    41161 ctgcgggtta aacccttccg gagatccttc tgagcaataa accagagttt tagcctgaac
    41221 acttgctgcg acggtcatag ccaccaggct gagaccaagc ttcagcatcc ctgacttttt
    41281 caaggaaata cgcattattc tgctccaatt gtgatgtttg ttgttttaac cctttgcagt
    41341 gggttgtcgc tgcctgacct tttttgtttt ttgcccggtc gggtcaacgt tatgaggtgg
    41401 ggatgccgta ctaattaaca tcagtgtaac agtgcggatc ctccataaaa tgcccctcgt
    41461 gacctacaat ctgtcaacag aatgtgaaaa cgtcaataca gccaaccggg atttacacca
    41521 acggtgagaa tccacaaaca aagattaaaa aaacttcaaa cagctatttg cagcagagaa
    41581 atttgtgcta ctccaacatg accagaacaa tcagcttaat atttagcaac atttggtgat
    41641 taagttttat gcatttaatg aaaaaaatct gaggaaaagg tggatatctg agcgattaat
    41701 accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc agccataagt aacgcaaaaa
    41761 aagatttaag caaatataaa aaaagacaat ggtttctgtc acaaatccgt taatggatag
    41821 tgagatatgg ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga ataaggcggt
    41881 cgcgccgcat ccgacaataa gtgcggtgcc gttgcctgat gcgacgctg
//
LOCUS       DAZFRJ010000017        41513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000017 DAZFRJ010000000
VERSION     DAZFRJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.017"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            253..339
                     /gene="leuL"
                     /locus_tag="ACHKUN_000847"
     CDS             253..339
                     /gene="leuL"
                     /locus_tag="ACHKUN_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO6659157.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            432..2003
                     /gene="leuA"
                     /locus_tag="ACHKUN_000848"
     CDS             432..2003
                     /gene="leuA"
                     /locus_tag="ACHKUN_000848"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO6659158.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2003..3094
                     /gene="leuB"
                     /locus_tag="ACHKUN_000849"
     CDS             2003..3094
                     /gene="leuB"
                     /locus_tag="ACHKUN_000849"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO6659159.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3097..4497
                     /gene="leuC"
                     /locus_tag="ACHKUN_000850"
     CDS             3097..4497
                     /gene="leuC"
                     /locus_tag="ACHKUN_000850"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO6659160.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4508..5113
                     /gene="leuD"
                     /locus_tag="ACHKUN_000851"
     CDS             4508..5113
                     /gene="leuD"
                     /locus_tag="ACHKUN_000851"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO6659161.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACHKUN_000852"
     CDS             complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACHKUN_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO6659162.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACHKUN_000853"
     CDS             complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACHKUN_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO6659163.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6662..8317
                     /gene="sgrR"
                     /locus_tag="ACHKUN_000854"
     CDS             6662..8317
                     /gene="sgrR"
                     /locus_tag="ACHKUN_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO6659164.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8351..8450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8481..9464
                     /gene="thiB"
                     /locus_tag="ACHKUN_000855"
     CDS             8481..9464
                     /gene="thiB"
                     /locus_tag="ACHKUN_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO6659165.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9440..11050
                     /gene="thiP"
                     /locus_tag="ACHKUN_000856"
     CDS             9440..11050
                     /gene="thiP"
                     /locus_tag="ACHKUN_000856"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO6659166.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11034..11732
                     /gene="thiQ"
                     /locus_tag="ACHKUN_000857"
     CDS             11034..11732
                     /gene="thiQ"
                     /locus_tag="ACHKUN_000857"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO6659167.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACHKUN_000858"
     CDS             complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACHKUN_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6659168.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACHKUN_000859"
     CDS             complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACHKUN_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO6659169.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13913..15613
                     /gene="araB"
                     /locus_tag="ACHKUN_000860"
     CDS             13913..15613
                     /gene="araB"
                     /locus_tag="ACHKUN_000860"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO6659170.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15624..17126
                     /gene="araA"
                     /locus_tag="ACHKUN_000861"
     CDS             15624..17126
                     /gene="araA"
                     /locus_tag="ACHKUN_000861"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO6659171.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17295..17990
                     /gene="araD"
                     /locus_tag="ACHKUN_000862"
     CDS             17295..17990
                     /gene="araD"
                     /locus_tag="ACHKUN_000862"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6659172.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18065..20416
                     /gene="polB"
                     /locus_tag="ACHKUN_000863"
     CDS             18065..20416
                     /gene="polB"
                     /locus_tag="ACHKUN_000863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO6659173.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20581..23487
                     /gene="rapA"
                     /locus_tag="ACHKUN_000864"
     CDS             20581..23487
                     /gene="rapA"
                     /locus_tag="ACHKUN_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO6659174.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23499..24158
                     /gene="rluA"
                     /locus_tag="ACHKUN_000865"
     CDS             23499..24158
                     /gene="rluA"
                     /locus_tag="ACHKUN_000865"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO6659175.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24275..25090)
                     /gene="djlA"
                     /locus_tag="ACHKUN_000866"
     CDS             complement(24275..25090)
                     /gene="djlA"
                     /locus_tag="ACHKUN_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO6659176.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25345..27699
                     /gene="lptD"
                     /locus_tag="ACHKUN_000867"
     CDS             25345..27699
                     /gene="lptD"
                     /locus_tag="ACHKUN_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO6659177.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27752..29038
                     /gene="surA"
                     /locus_tag="ACHKUN_000868"
     CDS             27752..29038
                     /gene="surA"
                     /locus_tag="ACHKUN_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO6659178.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29038..30027
                     /gene="pdxA"
                     /locus_tag="ACHKUN_000869"
     CDS             29038..30027
                     /gene="pdxA"
                     /locus_tag="ACHKUN_000869"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO6659179.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30024..30845
                     /gene="rsmA"
                     /locus_tag="ACHKUN_000870"
     CDS             30024..30845
                     /gene="rsmA"
                     /locus_tag="ACHKUN_000870"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO6659180.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30848..31225
                     /gene="apaG"
                     /locus_tag="ACHKUN_000871"
     CDS             30848..31225
                     /gene="apaG"
                     /locus_tag="ACHKUN_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO6659181.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31232..32080
                     /gene="apaH"
                     /locus_tag="ACHKUN_000872"
     CDS             31232..32080
                     /gene="apaH"
                     /locus_tag="ACHKUN_000872"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO6659182.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32105..32704
                     /locus_tag="ACHKUN_000873"
     CDS             32105..32704
                     /locus_tag="ACHKUN_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO6659183.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32740..33207
                     /locus_tag="ACHKUN_000874"
     CDS             32740..33207
                     /locus_tag="ACHKUN_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659184.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33306..33485)
                     /locus_tag="ACHKUN_000875"
     CDS             complement(33306..33485)
                     /locus_tag="ACHKUN_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO6659185.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33506..33985)
                     /gene="folA"
                     /locus_tag="ACHKUN_000876"
     CDS             complement(33506..33985)
                     /gene="folA"
                     /locus_tag="ACHKUN_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO6659186.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34177..36039)
                     /gene="kefC"
                     /locus_tag="ACHKUN_000877"
     CDS             complement(34177..36039)
                     /gene="kefC"
                     /locus_tag="ACHKUN_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO6659187.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36032..36562)
                     /gene="kefF"
                     /locus_tag="ACHKUN_000878"
     CDS             complement(36032..36562)
                     /gene="kefF"
                     /locus_tag="ACHKUN_000878"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO6659188.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36670..38001)
                     /gene="yaaU"
                     /locus_tag="ACHKUN_000879"
     CDS             complement(36670..38001)
                     /gene="yaaU"
                     /locus_tag="ACHKUN_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6659189.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38060..38347)
                     /gene="fixX"
                     /locus_tag="ACHKUN_000880"
     CDS             complement(38060..38347)
                     /gene="fixX"
                     /locus_tag="ACHKUN_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO6659190.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38344..39630)
                     /gene="fixC"
                     /locus_tag="ACHKUN_000881"
     CDS             complement(38344..39630)
                     /gene="fixC"
                     /locus_tag="ACHKUN_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6659191.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39681..40622)
                     /gene="fixB"
                     /locus_tag="ACHKUN_000882"
     CDS             complement(39681..40622)
                     /gene="fixB"
                     /locus_tag="ACHKUN_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6659192.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40637..41407)
                     /gene="fixA"
                     /locus_tag="ACHKUN_000883"
     CDS             complement(40637..41407)
                     /gene="fixA"
                     /locus_tag="ACHKUN_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO6659193.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10047 a        11390 c        10847 g         9229 t
ORIGIN      
        1 aacgcgtgca ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat
       61 atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca
      121 ccgcagcaca attagctaat tttacgggtg cagaactcac gctggcggga cgtttttatt
      181 gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct
      241 ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct
      301 tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa
      361 aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa
      421 ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac
      481 aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc
      541 gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat
      601 cggtgcaaac catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg
      661 tggaaaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc
      721 atacctttat tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc accctggacg
      781 aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg
      841 aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag
      901 cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc
      961 cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag
     1021 ccattatttc cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg
     1081 cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg
     1141 gtaactgttc gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg
     1201 tccacaccgc cattaatcac caggaaatat ggcgcaccag ccagttagtt agccagattt
     1261 gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc gcacactcct
     1321 ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag
     1381 aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg
     1441 tgaaacatcg catggacgag atggggtata aagaaagtga atataattta gacaatttgt
     1501 acgacgcctt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg
     1561 cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca
     1621 gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag
     1681 aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta
     1741 accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg
     1801 gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg
     1861 gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga
     1921 acaatatctg gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg
     1981 aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga
     2041 cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg
     2101 ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggtgcagcca ttgataacca
     2161 cgggcaacca ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt
     2221 tggctcggta ggcggtccga aatgggaaca tttaccacca gaccagcaac cagaacgcgg
     2281 cgcgctgttg cctttgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact
     2341 gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcta acggcttcga
     2401 catcctgtgc gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga
     2461 aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagatcga
     2521 gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat
     2581 cgacaaagct aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc
     2641 cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct
     2701 gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct
     2761 gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa
     2821 cgagcaaggt tttggtctgt atgaaccggc aggcggctca gcaccagata tcgcaggcaa
     2881 aaacatcgcc aacccgattg cgcaaattct gtcgctggca ctgctgctgc gctacagcct
     2941 ggatgccgat gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg
     3001 cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga
     3061 tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa
     3121 ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac
     3181 cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt
     3241 cgcccggtac gtcagccggg caaaaccttc gccactatgg atcacaacgt ctctacccag
     3301 acgaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa
     3361 aactgcaaag aatttggcgt cgaactgtat gacctgaatc acccgtatca ggggatcgtc
     3421 cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac
     3481 tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa
     3541 gttgaacacg tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac catgaaaatt
     3601 gaagtccagg gcaaagccgc gccgggcatt accgcaaaag atatcgtgct ggcaattatc
     3661 ggtaaaaccg gtagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc
     3721 cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca
     3781 aaagccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtttgcat
     3841 gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac
     3901 gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc
     3961 acctggggca ccaatccagg ccaggtgatt tccgtgaacg acaatattcc cgatccggct
     4021 tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg
     4081 aaaccgggca ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc
     4141 aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg
     4201 ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg
     4261 gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca
     4321 atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc
     4381 aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca
     4441 atggctgccg ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga
     4501 gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc
     4561 caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg
     4621 ttttggcgcg catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa
     4681 cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga
     4741 aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt
     4801 taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca
     4861 gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc
     4921 caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa
     4981 aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag
     5041 tattgggctt accttgcagc acgacgacgc cattgccgct tatgaagcaa aacaacctgc
     5101 gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc
     5161 atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta
     5221 cccagtagac agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca
     5281 gaattacccc ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag
     5341 gcattaaatc ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt
     5401 taaaaagttg cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccggtgtaaa
     5461 acagtactcc tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat
     5521 agtagccagc cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac
     5581 ccgccagttt gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt
     5641 acatggtgtt gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat
     5701 cctgccagcc accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag
     5761 acggaagcat aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa
     5821 acatcaccgt aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc
     5881 atgccagaga aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt
     5941 tatccgcata ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg
     6001 ccagaagcac accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg
     6061 cattgccgat agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt
     6121 cagaacgttt tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa
     6181 agagtccgat ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag
     6241 gagcctgtag cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa
     6301 caccgttcat acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg
     6361 cgagaataaa aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca
     6421 gtcacacatg atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagttc
     6481 ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt
     6541 cgcggtaaaa tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg
     6601 aattattgac ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt
     6661 tatgccatct gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa
     6721 atcgcaggac acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat
     6781 gcgtaccctg ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg
     6841 gcgcggtaaa cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg
     6901 ggcggaagac ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa
     6961 agcgactgtg cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca
     7021 catcctgcgc gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg
     7081 ccgttccgaa acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga
     7141 aaatggggaa ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg
     7201 gcgtttcttt ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga
     7261 cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgcggacat
     7321 tgtgtcgccg acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc
     7381 gttactgctg ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa
     7441 ctttgccagc catcccatcg gcaccggtcc gtatgcggtg atccgcaaca ccaccaatca
     7501 actgaaaatt caggcattcg atgacttctt cggttaccgg gcattaatcg atgaagttaa
     7561 cgtctgggtt ctgccggaaa ttgccgacga gccagctgga ggtctgatgc taaaaggtcc
     7621 acagggtgag gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt
     7681 cgacagccgc acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct
     7741 ttctccaact aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc
     7801 ttatggactg ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg
     7861 cctggaaagc ctcatcctga ccttttatca ggatcacagt gagcatcggg tgattgccgg
     7921 gatcatgcag cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta
     7981 cgatcagtgg catgaaggag agatcgagag cgatatctgg cttaacagcg ccaactttac
     8041 gctgcctctg gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg
     8101 tattccgatc gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc
     8161 gaactggtgc cagcaactgg tcgccagcaa agcaatggtg ccgcttatcc accactggct
     8221 gatcattcag gggcaacgca gtatgcgcgg cctgcgcatg aacaccctcg gctggttcga
     8281 ttttaaatca gcgtggtttg cgccaccgga tccatgattg ctggtagata acaaaatcac
     8341 tacactaacg ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt
     8401 cgaacctgat ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc
     8461 actctttttt gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag
     8521 cgcccgtttt cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact
     8581 gggggcctgg tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc gaactgaaac
     8641 tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata
     8701 gtaaagccga tgtggtgctg gggctggata acaatctgtt agacgccgcc agtaaaaccg
     8761 gactatttgc caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata
     8821 atgacacttt cgtcccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac
     8881 tgaaaaaccc gccgcaaagc ctgaaagaat tggttgagag cgatcagaac tggcgggtga
     8941 tttatcagga tccgcgcacc agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag
     9001 tctatggcga tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca
     9061 ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga
     9121 gttacaccac ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg
     9181 cgaactttag cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca
     9241 agcagccgga gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg
     9301 cgatcccaac cggtaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg
     9361 aacaattgac caaaccggcc accacgctgg agttcacacc agctgaagtg gctgcacaac
     9421 gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat
     9481 tccaggtgta agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct
     9541 gtggtggaac gcgccgcagg gtaactgggt ggcagtctgg caggacagct acctgtggca
     9601 tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc
     9661 gatattcctc gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg
     9721 tctgtgcgca atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt
     9781 ctatggtcgt cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt
     9841 ttcgccctac ggcctgcaag gcattttgct ggcgcacgta ttttttaatc tgccgatggc
     9901 gagccgctta ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc
     9961 ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg
    10021 acaaatcccg ccggttgccg cgcttatctt tatgctctgt ttcgccagct tcgccaccgt
    10081 gctatcgctg ggcggcggtc cgcaggcaac tactatcgag ctggctattt atcaggcgct
    10141 gagttacgac tacgatcctg cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg
    10201 ccttgggctg gtgctgttga gtcagcgact aagtaaggcc attgcgcccg gtaccacgct
    10261 gctgcaaggc tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt
    10321 aatagtgctg gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatggggt
    10381 aaatcgccag ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc
    10441 gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg
    10501 gagcagtcgc gaactgcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag
    10561 cggcatgttg atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct
    10621 caacaacact atcggcctgc cacaatctgc tgacggcatt gtgattttca ccaatgcgtt
    10681 aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg
    10741 ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga
    10801 gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat
    10861 tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta
    10921 tctctaccag caaattggct cctatcgcag ccaggacggc gcggtcaccg cgttaattct
    10981 gctactactc tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa
    11041 aactgactga tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag
    11101 aacgcggcga gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga
    11161 atttgattgc cggttttctg acgccagcca gcggttcgct gactatcgac agcgttgatc
    11221 acaccacgac gccgccgtca cgccgcccgg tatcgatgct gtttcaggag aacaacctgt
    11281 tcagccacct gacggtcgcg cagaacatcg ggctggggct taatccggga ttgaagctga
    11341 acgcggcgca gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa
    11401 tggcgcggtt accgggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt
    11461 gtctggtgcg cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg
    11521 cgttacgtca ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc
    11581 tattgatggt gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg
    11641 tcgccgacgg gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga
    11701 gtgcttcggc actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc
    11761 cttatccgac ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat
    11821 caggcaaccc gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat
    11881 acaccggcat caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa
    11941 ttaacggcgt cagccacaac aaacggccgc gcgacaaata atgacttaaa cgatcggttg
    12001 ctttaccgct gcgccataac cgccagcaca gccagccacc aacccacaaa aataccgccg
    12061 tcgccagcag caaccattta aactcgccgc tctgcattcc ggctggaata tcgatcgccg
    12121 cgcccgccag aatcccaggc aggaagtaaa acggtggcca cagcaggcag ccgataatat
    12181 tcggcgtaat gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg
    12241 gacgcgtcgg gccaacaaag cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg
    12301 catgttcagt tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg
    12361 gctttttaaa acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga
    12421 tccctgccag ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc
    12481 ccgccatcag caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg
    12541 ccaccaacac caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca
    12601 atgcttgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
    12661 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
    12721 ttcacttttt cttcacaacc ggctcggaac tcgctcgggc tggccccggt gcatttttta
    12781 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
    12841 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
    12901 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
    12961 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
    13021 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
    13081 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
    13141 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
    13201 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
    13261 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
    13321 taacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
    13381 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
    13441 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
    13501 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
    13561 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
    13621 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta
    13681 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
    13741 aacaaaagtg tctataatca cggcagaaaa gtccacattg gttatttgca cggcgtcaca
    13801 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
    13861 tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat
    13921 tgcaattggc ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag
    13981 cggtgaagag atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg
    14041 tgatgccccg aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc
    14101 ggcactgaaa accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat
    14161 tggcgttgac agtaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc
    14221 gctgcgcccg gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac
    14281 tgcggttgaa gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta
    14341 ctcccgctac attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt
    14401 gactcgccag gacaccgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg
    14461 ggtgccagct ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag
    14521 cgccgggcat aagtcgctat ggcacgaaag ctggggtggc ttgccgccag ccagtttctt
    14581 tgatgagctg gacccgatcc tcaatcgcca tttaccttcc ccgctgttca ctgacacctg
    14641 gactgccgat attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc
    14701 tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg
    14761 cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc
    14821 cgacaaacag agcgttggcg agcgggcagt taaaggtatt tgcggtcagg ttgatggcag
    14881 cgtggtacct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc
    14941 ctggtttggt cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact
    15001 gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc
    15061 caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg gtcgtcgcac
    15121 accgaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc
    15181 tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga
    15241 gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg
    15301 gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt
    15361 tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt
    15421 gcacgcagac atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca
    15481 accgcgcagc gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc
    15541 gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt
    15601 tgcgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca
    15661 ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc
    15721 acgttgttaa tgcgctgaat acggaagcga aactgccctg caaactagtg ttgaaaccgc
    15781 tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt
    15841 gcgctggtct ggtggtgtgg ctgcacactt tctccccggc caaaatgtgg atcaacggcc
    15901 tgaccatgct caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt
    15961 gggacagtat cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt
    16021 tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt cactggcagg
    16081 ataaacaagc ccatgagcgt atcggctcct ggatgcgcca ggcggtctct aaacaggata
    16141 cccgtcatct gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg gtcaccgatg
    16201 gtgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg
    16261 atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt
    16321 acgaaagctg ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg
    16381 tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc
    16441 acgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg
    16501 tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg
    16561 ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg
    16621 aggactacac ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg
    16681 aagtctgccc gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta
    16741 ttggtggtaa ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg
    16801 tcgccagcct gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg
    16861 tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac
    16921 cggatctgcc aactgcttcc gaagcgtgga tccttgctgg tggcgcgcac cataccgtct
    16981 tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa
    17041 tcacggtgat tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg
    17101 aagtgtatta cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac
    17161 gctgacgcgt cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca
    17221 tccggcattg ttttccccct cgcccctttg gggagagggc cggggtgagg ggaaacgacc
    17281 tctcaggagt caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc
    17341 tgccaaaaca caacctggtc acgctcacat ggggcaacgt cagcgccgtt gatcgcgagc
    17401 gcggcgtctt tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata
    17461 tggtcgtggt tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg
    17521 acacgccaac tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata
    17581 cgcactcgcg ccacgccacc atctgggcgc aggcgggtca gtcgattcca gcaaccggca
    17641 ccacccacgc cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag
    17701 aaatcaacgg cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac
    17761 agggtatcga tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat
    17821 ggggcaaaaa tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata
    17881 tggagatatt ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg
    17941 ataaacacta tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata
    18001 aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt
    18061 cagcgtggcg caggcaggtt ttatcttaac ccgacactgg cgggacaccc cgcaagggac
    18121 agaagtctcc ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca
    18181 agagtccgtg gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg
    18241 tgaacaaggc tttcgcctga caccgctggc gttaaaggat tttcaccgcc agccggtgta
    18301 tggcctttac tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc tgcgtgaagg
    18361 tggcgttacc gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt
    18421 tatcacctca ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg
    18481 tctgaaaccg catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac
    18541 cacccgccac ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta
    18601 tatgctgggg ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc
    18661 cagccgcccg cagttgctgg aaaaactcaa cgcctggttt gccaactacg atcctgatgt
    18721 gatcatcggt tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg
    18781 ttaccgtctt ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg
    18841 ctttaaaaac ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga
    18901 ggcgctgaaa tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga
    18961 gctattaggc gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg
    19021 ccgtttcgcc gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt
    19081 gacgcagatc ttccacaaaa ctgaaatcat gccattttta ctcgaacgag caacggtgaa
    19141 cggcctgccg gtggatcggc acggcggttc ggtggcagcg tttggtcatc tctattttcc
    19201 gcgaatgcat cgcgctggtt atgtcgcgcc taatctaggc gaagtgccgc cgcacgccag
    19261 ccctggcggc tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga
    19321 ctataaaagc ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt
    19381 tgaaggcatg gcgcagcctg atccagagca cagtaccgaa ggttttctcg atgcctggtt
    19441 ctcacgggag aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga
    19501 agccaaacgt cagggtaata aaccactttc gcaggcgctg aagatcatta tgaatgcctt
    19561 ttatggcgtg cttggcacca ccgcctgccg ctttttcgat ccgcgcctgg catcgtcgat
    19621 caccatgcgt ggtcatcaga tcatgcggca aaccaaagca ttgattgaag cgcagggcta
    19681 cgacgttatc tacggagata ccgactcgac ttttgtctgg ctaaaaggtg cacattcgga
    19741 agaagaggcg gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct ggtgggcgga
    19801 aacgctgcaa aaacaacggc tgaccagcgc attagaactg gagtatgaaa ctcatttctg
    19861 ccgttttctt atgccaacca ttcgcggagc tgataccggc agtaaaaagc gctacgccgg
    19921 gctgattcag gagggcgaca agcagcggat ggtgtttaaa gggctggaaa ccgtgcgcac
    19981 cgactggacg ccgctggccc agcagtttca gcaggagctg tacctgcgta tcttccgcaa
    20041 cgagccatat caggagtacg tccgccaaac catcgacaaa ctgatggcgg gtgaactgga
    20101 tgcgcggctg gtttaccgta aacgccttcg ccgtccgctg agcgaatatc agcgtaatgt
    20161 tccgcctcat gtacgcgccg ctcgccttgc cgatgaagaa aatcaaaagc gcggtcgccc
    20221 cttgcaatat cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc
    20281 gctggactac caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc
    20341 cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca
    20401 acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa
    20461 tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata acgccactac
    20521 ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
    20581 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga
    20641 accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac
    20701 cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc
    20761 attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg
    20821 ctgacctata tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt
    20881 ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt
    20941 gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc
    21001 cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgatcccgca tcagctcaac
    21061 atcgctcatg atgttggtcg ccgccacgcg ccgcgcgttc tgctggctga cgaagtgggt
    21121 ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct
    21181 gaacgtgtgc tgattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg
    21241 cgccgtttca acctgcgttt tgcgctgttt gatgatgagc gttatgccga agctcagcac
    21301 gatgcttaca acccgttcga caccgaacag ctggtgattt gctcgctgga ttttgcccgt
    21361 cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat
    21421 gaagcgcatc acctggtgtg gagcgaagac gcgccgagcc gcgaatatca ggctattgaa
    21481 caactggcag agcacgtgcc gggcgttctg ctgctgaccg caaccccgga acagttggga
    21541 atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg
    21601 cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg
    21661 gcaggtaaca aactgagcaa tgacgaactg aacatgcttg gcgagatgat cggcgagcag
    21721 gatatcgagc cgctgttgca agcagcaaac agcgacagcg aagatgccca gagcgcccgt
    21781 caggaactgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac
    21841 acccgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa gctgccgcta
    21901 ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg
    21961 gaagatcgtg ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat
    22021 aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc
    22081 catcgctctc agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag
    22141 caggtactgc gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt
    22201 atcgaacgtg accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg
    22261 ctgtgctcag aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtaatg
    22321 tttgacctgc cattcaaccc ggatctgctg gagcagcgta ttggtcgtct ggatcgtatc
    22381 ggtcaggcgc acgatattca gatccatgtg ccttatctgg aaaaaactgc tcagtcggtg
    22441 ctggtgcgct ggtatcacga aggtctggat gcattcgagc acacctgccc gaccggacgc
    22501 actatttacg atagcgtcta caacgatctg attaactatc tggcttcacc ggatcaaacc
    22561 gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacaa
    22621 ctggaacagg ggcgtgatcg cctgctggaa atccactcca acggtggcga aaaagcccag
    22681 gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg
    22741 aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg
    22801 accccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc
    22861 atcacctttg atcgtgaagt ggcgctggcg cgtgaagatg ctcagtttat tacctgggaa
    22921 catccgctga tccgcaacgg tctggatctg attctttctg gcgataccgg tagcagcacg
    22981 atttcactgt taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttac
    23041 gtggtcgaag cccaggcgcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg
    23101 gtacgtatgc tgctggataa aaacggcaac aacctggcgg cccaggtgga gtttgaaacc
    23161 tttaaccgcc agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg
    23221 cagcaggatg ttcacgctat ccttcagctg ggtgaagcgc agatcgagaa atctgcccgt
    23281 gcattgattg atgccgcacg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt
    23341 ctggaagcgc tgcgcgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag
    23401 agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag gctggcgtct ggatgccctg
    23461 cgtttgatcg ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc
    23521 caccgcagga accctggttg gttatcctgt atcaggatga ccacattatg gtggttaaca
    23581 agccgagcgg tttgttgtct gtgccgggtc gtctggaaga gcacaaagat agcgtgatga
    23641 cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca
    23701 gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc
    23761 gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag
    23821 aaggtctggt ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct
    23881 gttacgaaac gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gtggcggata
    23941 acacggcaag agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata
    24001 tgctggcgct gggtcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag
    24061 cgatggcacc acgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg
    24121 gcaatagtat gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg
    24181 taggcggata aggcgttcac gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg
    24241 cgggccgcgt cttatcaggc ctacagttta aaacttattt aaaccctttc tgctgcttta
    24301 tcagctcata tgcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag
    24361 gcggcaaacc tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg
    24421 cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta caggcatctt
    24481 ccagcgtcgg gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac
    24541 cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg
    24601 agatccctaa ttcttcagca atgacataca gcaccgcccg ttcattcggg tgcagtgaac
    24661 catcagcaaa cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac
    24721 caaagcagac actgcgaaac tggcgcatct tttcgcgcag cgggtaatta tctgattttc
    24781 ccactcggaa cgcgttttgc gccgcagtac gggaagcgcc atgaaggttc attcggtcca
    24841 tcaactggct ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat
    24901 gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga
    24961 accacgccat tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca
    25021 ctacgcccca aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc
    25081 aatactgcat atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa
    25141 aggtgctttt accgttttcc ggctgcggtc aattgtggga catcgcctat aatttgcatt
    25201 atcatacctg tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac
    25261 actagcgtag agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg
    25321 atgatggaac aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg
    25381 attgccaccg ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg
    25441 ttgggcgtgc caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg
    25501 actatcaatg ctgaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc
    25561 gtggatatca tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa
    25621 gaggcaccag gacaaccgga gccggtacgt accgttgatg cgctcggtaa tgtccattac
    25681 gacgataacc aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat
    25741 accaacgtct gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac
    25801 ctgatgaaac aacgtggcga aaaccgctat accattctgg ataacggtag ctttacctcc
    25861 tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa
    25921 gaacaagttg cggagatctg gaacgcccgc tttaaggtgg gtccggtacc gatcttttat
    25981 agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac
    26041 gccaagtaca ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg
    26101 ccaaatatgg atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg
    26161 gagaacgaat tccgctacct ctcccaggcg ggcgctggct tgatggaact ggactatctg
    26221 ccttcagata aagtctatga agatgaacac ccgaacgatg acagttcacg tcgttggtta
    26281 ttctactgga accactccgg ggtcatggat caggtgtggc gtttcaacgt cgactacacc
    26341 aaggtcagcg atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac
    26401 ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt
    26461 tcaaccaagc agttccaggt tttcagcgaa cagaacacca gtagctactc ggcagagccg
    26521 cagttagacg ttaattacta ccagaatgat gttggtccat ttgatacgcg tatttacggc
    26581 caggcagtgc actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg
    26641 gaaccgacca tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag
    26701 ttgctggcaa cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc
    26761 aagctggacg aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc
    26821 tttgaacgcg atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg
    26881 cagtatttgt acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctctg
    26941 ctgcaatctg actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt
    27001 gcctccgcta accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt
    27061 gaacgtttta atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat
    27121 gacaacataa catgggagaa tgacgacaaa acgggttcac tggtgtgggc aggcgatact
    27181 tactggcgta tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg
    27241 gataacgtag cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta
    27301 cagctgaatt accgttacgc cagcccggaa tatattcagg ctacgctgcc taagtactat
    27361 tccactgctg agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca
    27421 attgccgatc gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa
    27481 gccgactcta tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac
    27541 gagcggaagc tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc
    27601 ggctttaaca tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg
    27661 ctgcgttcga acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat
    27721 ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg
    27781 tatcgccatg atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc
    27841 cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa
    27901 actgaacgct gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc gccaccaaat
    27961 catggaacgt ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa
    28021 aatctccgat gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac
    28081 gctggatcag atgcgcagcc gtctggctta cgatggactg aactacaaca cctatcgtaa
    28141 ccagatccgc aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat
    28201 caccatcctg ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc
    28261 cagcactgag ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacctctga
    28321 tcaggtgaac gaagcggaaa gccaggcgcg cgccattgtc gatcaggcgc gtaacggcgc
    28381 tgatttcggt aagctggcga ttgctcattc tgccgaccag caggcgctga acggcggcca
    28441 gatgggctgg ggccgtattc aggagttgcc cgggatcttc gcccaggcat taagcaccgc
    28501 gaagaaaggc gacattgttg gcccgattcg ttccggcgtt ggcttccata ttctgaaagt
    28561 taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat
    28621 tctgctgaaa ccgtcgccga tcatgactga cgaacaggcc cgtgtgaaac tggaacagat
    28681 tgctgctgat atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca
    28741 ggatccaggc tctgctaacc agggcggtga tctcggctgg gctacaccag atattttcga
    28801 tccggccttc cgtgacgccc tgactcgcct gaacaaaggt caaatgagtg caccggttca
    28861 ctcttcattc ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga
    28921 cgctgcgcag aaagatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc
    28981 agcaagctgg atgcaggaac aacgtgccag cgcctacgtt aaaatcctga gcaactaatg
    29041 gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta
    29101 gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgatgccact
    29161 ctccttaccg accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc
    29221 aactcccctg cacaaccgca aactacgggc acattaacgc tacttcctgt cgcgctacgt
    29281 gaatctgtca ctgcggggca gttagcgatt gaaaatggac attacgtggt ggagacgctg
    29341 gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg
    29401 cataaaggcg tcattaacga cgcaggcatt ccgtttaccg gtcataccga gtttttcgaa
    29461 gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg
    29521 ctggcaacga cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg
    29581 cacgaagtga ttgctatttt gcatcacgat ttgcgtacaa aatttggtat tgccgaaccg
    29641 cgcattctgg tctgcgggct gaatccgcac gcgggcgaag gcggtcatat gggtacggaa
    29701 gagatagaca ccattattcc ggtgctcgac gagctgcggg cgcaggggat gaaactcaac
    29761 gggccgctgc ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg
    29821 ctggcgatgt accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt
    29881 gtgaacatta cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcgctt
    29941 gaactggcgg gacgtggcga agccgatgtc ggcagtttta ttacggcgct taatctcgcc
    30001 atcaaaatga ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagcccgta
    30061 aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat cgacagcatt gtttctgcca
    30121 ttaacccgca aaagggccag gcaatggtcg aaatcggccc cggtctggcg gcattgaccg
    30181 agccggtcgg cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg
    30241 cacgtctgca aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga
    30301 tgacctttaa ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca
    30361 acctgcctta taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca
    30421 ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga
    30481 acagcaaagc gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg
    30541 tactggaagt tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc
    30601 gcctggttcc tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca
    30661 tcaccaccga agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt
    30721 ttagcgtcga ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg gaaaatatct
    30781 ctgtcgcgca atattgccag atggcgaact atctggcgga gaacgcgcct ttgcaggaga
    30841 gttaacgatg atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc
    30901 acaatcttca cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct
    30961 ggggcgagcg ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg
    31021 tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga
    31081 agagtaccag tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca
    31141 ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact
    31201 cgccgttccc acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca
    31261 tggttgttac gatgaactgt tcgcattgct gcataaagta gaatttaccc ctgggaaaga
    31321 taccctctgg ctgacgggcg atctggtcgc gcgcgggccg ggttcgctgg atgttctgcg
    31381 ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct
    31441 gctggcggta tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct
    31501 ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat
    31561 cgacgaagag aaaaagttgg tgatggccca cgcagggatc acgccgcagt gggatctaca
    31621 gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgcta tcgagtgact cctatccctt
    31681 ctttctcgac gctatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct
    31741 gggaagactg cgttttatca ccaacgcctt tacccgtatg cgtttttgct ttccaaacgg
    31801 tcaactggat atgtacagca aagaatcgcc ggaagaagcc cctgccccac tgaaaccgtg
    31861 gtttgcgatt cctggccctg tcgctgaaga atacagcatc gcctttggtc actgggcatc
    31921 gctggaaggc aaaggtacgc cggaaggtat ttacgcgctg gataccggct gctgctgggg
    31981 tgggtcatta acctgcctgc gctgggaaga taagcagtat tttgtccagc cgtcgaaccg
    32041 gcataaggat ttgggtgagg gagaggcggt cgcgtcttaa cgaattacca ttcagggagg
    32101 aataatggct gactattttg aaatacgtgc tgattacaat cagcacacca tcacgattta
    32161 tcaggcttac aatgatgcaa tcgctgacgt cgccgtacgt gacgggcgat ttggcgcacc
    32221 tttttcattc aaccgcatga cctggattaa accctctttt atgtggatga tggagcgtag
    32281 caactgggga ctgaaaaaag atcaacaaca cattctcgca atccgaatca aacgcacctt
    32341 ctttgataca ttgctggaac aagcggtgct aaccacgccg gaagcccatg tgtatccaca
    32401 tgcaggaatt tgggaaacgt tatttgcaca agcaaatgtg tatgttcagt gggacccaga
    32461 acgcagcata aatggtaaaa aactggagca ccgatcatta cagcttggta tttctcgtaa
    32521 cttgatcagt caatttaacg aagacgcgat tgttgctatt gatgatctga cgccgctggt
    32581 gcgaaagtgt cataaccttt tgataaacgg caaaacaaca caggcgaaat ccttcctgcc
    32641 gccagaaaaa atttacccag ttagtgcagc agcgagaaag gctttgggaa tgaaaaattc
    32701 ctgagacaaa tacgcaccta ttaagcggaa aatatctaag tgcgacaaat caacaccggg
    32761 cttaatacca gagaaattat tctggaaatg attgacgaag cagatgcccc cccgtttgct
    32821 gagcagcgga cctggcatcg caggcgaatc gcttttctat gtgattgcat cagcgttgct
    32881 gtcgactacg gcttcttctg ttctgtctct cagcagtcaa taatgaacat aaccaagatg
    32941 gctgaagatg tatggataac agaaagtaaa acgacccaac tcattgatat ttggcaacaa
    33001 cttaaacagc tttatccgcc agaaactatt ccctacgccc agaagttacc cactaaggaa
    33061 ttgcttgaac tgctactttc tagtcctgac gatcttcgaa taattgaaat tcctgacatc
    33121 atcggtgatg tttttgatga ccaaacctgg attcgattat tcaaaaaaca ctttgctgat
    33181 gttattcagc tacgatgtga tgtgtaacaa tccaaaatat atcgccagac gatagaccaa
    33241 aaagaaagta tttagcagat actcaacatt ctgacttctg tacagaaata gcagagttta
    33301 aaaacttaaa acttccggta ctcatcagag aaacatccag tttcttctgc aaattcattt
    33361 aatgccgaga tggcatcctc gtttacttca ttccactgta gagtggcctg aaggtttaga
    33421 cctacgcaat gagcgcgact cacaaaatcg cgctcactgg taaaatcaaa cgtcttatcg
    33481 ttcaatttgt agtcctggca aaaaattacc gccgctccag aatctcaaag caatagctgt
    33541 gagagttctg cgcatcagca tcgtggaatt cgctgaatac cgattcccag tcatccggct
    33601 cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt
    33661 tctgcgcttt tggcaggaac tgctcataaa cgcgaccgcc gccaatcacc atgatttctg
    33721 gtacgtcacc acacgccgcg atggcttcat ccaccgactt cacccacgtt acgcgatcgt
    33781 ccgtacccgg ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgattg
    33841 attcccaggt atggcggccc ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg
    33901 cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataacg cgatctaccg
    33961 ctaacgccgc aatcagactg atcattgaga tttcccgata aaaaaaattg tcgccactat
    34021 acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa attttatatt
    34081 ctgctggcga gtccacgctc tctccctgga ctcgccgcat tacaatgaaa caaaaacaaa
    34141 cagttagctg taaagtgtga tttacgtcac tctttattag gatgagggtt tcgtttccgg
    34201 ttcatccgcc atgttgccgg tatgtttacc ttcttcggtt ccctgccagc catgtcgttg
    34261 aattaatgac agatgttcgc ggtcctcggt aatgatctca cttaacatcg cgctggtgcg
    34321 tttatagacc gccgcgcggg ctttggtgtc gttctcaacc attgccatct cttccaccat
    34381 ctgaatatta aagcggcgga acacatcggc acgttctcgc gcttcatacg gccccagacc
    34441 taaactttcc agtgccagac gcccggtttt cagcgcacct tcgaaggttt cacgctccgg
    34501 cttttcaacg cctgcctgac gcaaacgaat gtagtggtcg acatcgcggg cgcgggcaat
    34561 aatctgcaaa tgcgggaaat gttctttcac catctccgtc agttgcaggt tggtttgcgg
    34621 atcatcaata gcattaatca gcacttccgc tttcgccgct ccggcagatt ccagtaaatc
    34681 catccgcgtt gcatcgccat aaaacacttt cataccaaat ttacgcagcg tttcgatatg
    34741 gtccggatcg tgatcgagca ccaccatttt caccccgctg gagagcagta aacgtccggt
    34801 aatctgccca aaacgaccga atccggcgat aatcacgcgc ggctgttctt cgtcgatctc
    34861 atcggcttca cgcgcttcct cagtagaaga ttgctcaagg cgattgagga tcaccagcag
    34921 aatcggcgtt gctgccatcg acagcgccac cgccagggtc agcgatttcg cccactccgg
    34981 ctccagcaca ttcgccatct gcgccgcgcc aaataccaca aaggcaaact cactgccctg
    35041 ccctaacaac accgcaaacc aacgacgctg tttatttggc acttgcaacg gtcgggcaat
    35101 cagccacagc atggcgattt tgatgatgag gaaaccgagc agcaaaatga caatgcgcaa
    35161 tgggttttca agcagcgtgc caaagtctat cgacatgcca acaccgatga aaaacagccc
    35221 caacagcaaa cctttaaatg gttcgatatc gctctccagc gcatgacggt attccgagct
    35281 tgccagcagt acgcccgcca gaaacgcgcc catcgccatc gacaagccga cctcttccag
    35341 cagcaaacca aagccaaaca cgaggaataa cgccacggca ctaaacactt cccgcaagcc
    35401 agagcgggct acaaaacgca gcgccggacg cgtgacatag cgccccagca ataccaccag
    35461 caccagcgca cccgccactt ttaacgccga gagagcaaat gcgcccatcg tcgtcgaggc
    35521 actgctcgtt gccagtagcg gaatcatcgc caccagcggg atcgccgcga tatcctggaa
    35581 cagcagcacc gcaaaggcac tgcgacccat ttgcgtcacc atcagattgc gttcattcat
    35641 cgcctgcatg gcaatcgccg tagaggagag cgccagcgtc atgccgatca attccgcgac
    35701 ctgccagcgc agcccaagta acatgcagaa cagccccagc aggccgccgc aaatcaccat
    35761 ctgcaatgcg ccaccgccga acactgccgc acgcagcttc cacagccttt gtggatcgag
    35821 ttcgaggccg ataataaaca gcatcagcac caccccaatc tcggcaaagt gcagaataga
    35881 ttcggcatcg gtcaccagtc gcagccccca cgggccaata atgcagccgg cgatcaggta
    35941 gccaagtacc gatcccagac caagacgtac cgcaatgggt acaatcagcg ctgccgaacc
    36001 gagataaatc agcgcctgaa tcagcgtatg gctatccatg atgggcctcc tgccattcca
    36061 gcagacgttg cttatagtga cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg
    36121 tgcagtgcat ggcaaacggt ggcagccagt tcagtccgca gtagattgcc gtcgcctgta
    36181 gcggctgcga cagcacatca aagcccggat gcgcaccaat ttcaaaatgg ctttccccgc
    36241 cgccggtcgt caccgcccac agcaaatgtt tgccatgcag cgccgtgccg ccatgaccgt
    36301 aagcccagcc gtgggagaaa actttatcga tccaaagttt gaggagcgga ggaatgctgt
    36361 accactgcat cgggtgctgc cagacgatca gatcggcgcg agacagcgcc tcctgctcgg
    36421 cggcaatatc gatattgaag tcaggataga gttgataaag agagcgaatt tcgacgcctt
    36481 ccagcgtcct tgcctgttca agcatccgtt tattcgcatg ggaatgatgc ggatacggat
    36541 gcgcataaat tataagaatc ataggtagcc cgcgtgatta cgcctttgtt ttcccacaaa
    36601 gtgtagtcat atattcacgc ggcttatagt caataatatt gattcagaaa agagagaaat
    36661 ctgaacaatt taccccattc tctgcccgcg gatcgtcata ttgctggttt gcgccagtga
    36721 catccctcga gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc
    36781 cgcccccatt agcatcgtgt tactgatacc gtaattattg ataaagatcg gcagtgccca
    36841 ggtcgaaaca atggtgccaa tacggcttaa ggacataatc acgcccacgg cagaggcgcg
    36901 gatatctgtc gggaagagtt cattaggata gagccactgc aaattacccg gcccgccaga
    36961 gaaaaaggca tacaccgcaa aggccatcac taccagccag atccccatat ccgggatcag
    37021 ccccaaaacc gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg
    37081 tccggcagtg tttaaccaca gcatcggcgg aatacagccg agcataaaga acaggctaat
    37141 caccacgttc cccagcgccg cgtttttgcc aacacccaat cccaacaaac caacgatttg
    37201 cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac
    37261 aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg
    37321 ctgctcttca tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc
    37381 ttttactcgc cctttgcgta ataaccagcg gggagattca ggcagttcga atcgaccaat
    37441 caaaatcaac aaacagggga tcgccgcgct acccagcatc cagcgccagc cgccttccac
    37501 atcataaagc cagtagccga ccagatcggc gcaggtcgcg ccgacatacc acatggcggc
    37561 gataaagctg atggaaaacg cccgctgacg ggtactggag aactcggtga tcattgaggt
    37621 ggcgatggga taatctgcac cgatgacaat gccgataagt acccgcatca ccaacagttc
    37681 gacgggggat gaaacaaaca tcgtcgccac cgatatcacg ccgatggcga tgatatcaat
    37741 gaggaacatt ttgcgccgtc cgactttatc ggaaatatag ccaaacagcg atgtgccaac
    37801 gaacagcccg gcgagcgttc ccgcgcccaa caagccaatc cagtcagcgt ccagttttag
    37861 cgccggagtc agttgctcca gcgccacgcc aatcattacc agtacataac catccagaaa
    37921 cggaccaccg cttccccaca gcaaaatgcg gcggtgaatt gaggagaatt tgagatcgtc
    37981 aaagtttctg gacggttgca tgggttattc ctgttttttt gcaatgagag tcagtacggc
    38041 gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt
    38101 attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac
    38161 atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc
    38221 acgctttcac cagcagctcc agggcctgtt tatcggcatc ggccttcaca acaatgtgcg
    38281 gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag
    38341 aagtcataaa acggtcactc ctttcatgcc atccttgatc agattgatga agcccacttt
    38401 cttgccgtgg cggaggattt tcttgcgcat cagttccggc gcactgccgt caatggtgaa
    38461 caggtcacgc gccacgccca ccgccagttc cgggtagccg ctaaacatgc gtgggttatc
    38521 aaggaaggcc ggtagtttct ggtacatacg catatcgcgc agcgggccac tctcaagatg
    38581 ctgacgatat tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac
    38641 ggtttttgct gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc
    38701 gaggttcata cacattccgg cggcatcacc ggcaatcaat acgccgtcac caaccaactc
    38761 cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc
    38821 gcccgcgatc agcggtgcca ctgccggatg ctgtttgaaa tcttccagca tttgcggcac
    38881 cgattttttt gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtatt
    38941 ttcattggta taaaggaagc cgccgcccat caggccatcg gtgggtgatc ccgcaaacag
    39001 acaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtaa
    39061 ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc
    39121 cagcttttcg gcaaggatgg agttcactcc atcagcaagg atcaccgttt tcgcttcaat
    39181 cacatcgcca tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac
    39241 gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca gccaggcatc
    39301 aaatttactg cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccattgca
    39361 gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag
    39421 gcgttctacg ggggcggagt cggcgaaacc aggaataatg tgttccaggc tgtgggcata
    39481 gagacgcccg ccggtgacgt tcttggcacc tgcggaattg ccacgctcga taactaacac
    39541 ttgcgcccct tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc cagcaccgac
    39601 gatgatggcg tcaaagatat cttcggacat aagagctccc tgtcagcggc agggccaaaa
    39661 tgcagccctg ccagagtgga tcaacgcgct aaagctgcgg tcagcgccgg aaggatcttc
    39721 acggcgtcac caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatcttta
    39781 ttgatggcga aaatggtttg cgatgcatta gcaccaacca tgtgctggat ctgcccggag
    39841 atccccaccg ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt
    39901 tcgtgctcca tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct
    39961 atcgccttgc aaagctgttc tgccagcgca atgttctctt tgctgccaat accgcgaccg
    40021 acgctgacca ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt
    40081 gccgtgcggg taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg
    40141 cgtgacgcgt ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag taccgcatac
    40201 ggcgtggcaa tgcgttcttc gccaatcgcc agaccaccgt aaaccatgtg tttcactgtc
    40261 gctttaccgt cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga
    40321 taacccagtt ttgccgccag taatttgccg cgacgggtgt ttggcagcag caccaggccg
    40381 tctgcgccgt gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccga
    40441 tcgtccggtt tgccgtttaa tttccagaca tgattagctc cgagctggat tgcctgtacg
    40501 ccgtcggcat cattgaggac aaaggtgttg atttgattag ctaaagcctg cgcaccgttc
    40561 atcagttccg gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc
    40621 atagcatccc ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt
    40681 cttcgccgtc gccttcaatc acgatgcgct gacgttcgcg ctgtttcggc gcagcaacct
    40741 gttgttcaga ccaggcgtcc acggcgttaa aaccgatatc cgccgccgac catacctgga
    40801 ctggcttttt cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat
    40861 cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt tcatcttcca
    40921 gttcgcgctc aacggtgagg gtatctgcag tcagggagat aattttgctg acgccgttaa
    40981 ctgccggaat attgaggatt tcgcccacca gcagaccaac ctgctgggca taaaggtcgg
    41041 aagaaccatc gccacagagg atcaaatcaa agcctgcttt ctgggcggct gcagccagtg
    41101 cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt
    41161 catccgggcc gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc
    41221 ccacacttaa ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt
    41281 caatagcgtt cagatcgtat tggcttattt tggcatcggc tttgctgaag tctaatgaac
    41341 catcagcatt attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa
    41401 tcttcattgc atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta
    41461 cagatatcac gccctttaat ttggaaataa aactttaatc accaatattg aaa
//
LOCUS       DAZFRJ010000018        41430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000018 DAZFRJ010000000
VERSION     DAZFRJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.018"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..218)
                     /locus_tag="ACHKUN_000884"
     CDS             complement(<1..218)
                     /locus_tag="ACHKUN_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659194.1"
                     /translation="MSGKPAARQGDMTQYGSSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACHKUN_000885"
     CDS             complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACHKUN_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO6659195.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            793..2991
                     /gene="priA"
                     /locus_tag="ACHKUN_000886"
     CDS             793..2991
                     /gene="priA"
                     /locus_tag="ACHKUN_000886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO6659196.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3147..4172
                     /gene="cytR"
                     /locus_tag="ACHKUN_000887"
     CDS             3147..4172
                     /gene="cytR"
                     /locus_tag="ACHKUN_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO6659197.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4264..5223
                     /gene="ftsN"
                     /locus_tag="ACHKUN_000888"
     CDS             4264..5223
                     /gene="ftsN"
                     /locus_tag="ACHKUN_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO6659198.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5316..5846
                     /gene="hslV"
                     /locus_tag="ACHKUN_000889"
     CDS             5316..5846
                     /gene="hslV"
                     /locus_tag="ACHKUN_000889"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO6659199.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5856..7187
                     /gene="hslU"
                     /locus_tag="ACHKUN_000890"
     CDS             5856..7187
                     /gene="hslU"
                     /locus_tag="ACHKUN_000890"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO6659200.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7254..8180
                     /gene="menA"
                     /locus_tag="ACHKUN_000891"
     CDS             7254..8180
                     /gene="menA"
                     /locus_tag="ACHKUN_000891"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO6659201.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8273..8758
                     /gene="rraA"
                     /locus_tag="ACHKUN_000892"
     CDS             8273..8758
                     /gene="rraA"
                     /locus_tag="ACHKUN_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO6659202.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACHKUN_000893"
     CDS             complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACHKUN_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO6659203.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9513..10358
                     /gene="glpF"
                     /locus_tag="ACHKUN_000894"
     CDS             9513..10358
                     /gene="glpF"
                     /locus_tag="ACHKUN_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO6659204.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10381..11889
                     /gene="glpK"
                     /locus_tag="ACHKUN_000895"
     CDS             10381..11889
                     /gene="glpK"
                     /locus_tag="ACHKUN_000895"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO6659205.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12024..13034
                     /gene="glpX"
                     /locus_tag="ACHKUN_000896"
     CDS             12024..13034
                     /gene="glpX"
                     /locus_tag="ACHKUN_000896"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6659206.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13131..13877
                     /gene="fpr"
                     /locus_tag="ACHKUN_000897"
     CDS             13131..13877
                     /gene="fpr"
                     /locus_tag="ACHKUN_000897"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO6659207.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13882..14310)
                     /gene="uspD"
                     /locus_tag="ACHKUN_000898"
     CDS             complement(13882..14310)
                     /gene="uspD"
                     /locus_tag="ACHKUN_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO6659208.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14337..14636)
                     /gene="yiiS"
                     /locus_tag="ACHKUN_000899"
     CDS             complement(14337..14636)
                     /gene="yiiS"
                     /locus_tag="ACHKUN_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO6659209.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14848..15288)
                     /gene="yiiR"
                     /locus_tag="ACHKUN_000900"
     CDS             complement(14848..15288)
                     /gene="yiiR"
                     /locus_tag="ACHKUN_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6659210.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15389..15988
                     /gene="yiiQ"
                     /locus_tag="ACHKUN_000901"
     CDS             15389..15988
                     /gene="yiiQ"
                     /locus_tag="ACHKUN_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO6659211.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16096..16863
                     /gene="tpiA"
                     /locus_tag="ACHKUN_000902"
     CDS             16096..16863
                     /gene="tpiA"
                     /locus_tag="ACHKUN_000902"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO6659212.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16918..17673)
                     /gene="cdh"
                     /locus_tag="ACHKUN_000903"
     CDS             complement(16918..17673)
                     /gene="cdh"
                     /locus_tag="ACHKUN_000903"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO6659213.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17780..18769)
                     /gene="sbp"
                     /locus_tag="ACHKUN_000904"
     CDS             complement(17780..18769)
                     /gene="sbp"
                     /locus_tag="ACHKUN_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO6659214.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19088..20050)
                     /gene="pfkA"
                     /locus_tag="ACHKUN_000905"
     CDS             complement(19088..20050)
                     /gene="pfkA"
                     /locus_tag="ACHKUN_000905"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO6659215.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20231..21133)
                     /gene="fieF"
                     /locus_tag="ACHKUN_000906"
     CDS             complement(20231..21133)
                     /gene="fieF"
                     /locus_tag="ACHKUN_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO6659216.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21282..21782)
                     /gene="cpxP"
                     /locus_tag="ACHKUN_000907"
     CDS             complement(21282..21782)
                     /gene="cpxP"
                     /locus_tag="ACHKUN_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO6659217.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21932..22630
                     /gene="cpxR"
                     /locus_tag="ACHKUN_000908"
     CDS             21932..22630
                     /gene="cpxR"
                     /locus_tag="ACHKUN_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO6659218.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22627..24000
                     /gene="cpxA"
                     /locus_tag="ACHKUN_000909"
     CDS             22627..24000
                     /gene="cpxA"
                     /locus_tag="ACHKUN_000909"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO6659219.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24142..24816)
                     /gene="yiiM"
                     /locus_tag="ACHKUN_000910"
     CDS             complement(24142..24816)
                     /gene="yiiM"
                     /locus_tag="ACHKUN_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO6659220.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24965..25948)
                     /gene="kdgT"
                     /locus_tag="ACHKUN_000911"
     CDS             complement(24965..25948)
                     /gene="kdgT"
                     /locus_tag="ACHKUN_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO6659221.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26208..26828)
                     /gene="sodA"
                     /locus_tag="ACHKUN_000912"
     CDS             complement(26208..26828)
                     /gene="sodA"
                     /locus_tag="ACHKUN_000912"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO6659222.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27113..28147
                     /gene="rhaT"
                     /locus_tag="ACHKUN_000913"
     CDS             27113..28147
                     /gene="rhaT"
                     /locus_tag="ACHKUN_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO6659223.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28144..28992)
                     /gene="rhaR"
                     /locus_tag="ACHKUN_000914"
     CDS             complement(28144..28992)
                     /gene="rhaR"
                     /locus_tag="ACHKUN_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO6659224.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29066..29903)
                     /gene="rhaS"
                     /locus_tag="ACHKUN_000915"
                     /pseudo
     CDS             complement(29066..29903)
                     /gene="rhaS"
                     /locus_tag="ACHKUN_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            30191..31660
                     /gene="rhaB"
                     /locus_tag="ACHKUN_000916"
     CDS             30191..31660
                     /gene="rhaB"
                     /locus_tag="ACHKUN_000916"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO6659225.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31657..32916
                     /gene="rhaA"
                     /locus_tag="ACHKUN_000917"
     CDS             31657..32916
                     /gene="rhaA"
                     /locus_tag="ACHKUN_000917"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO6659226.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32999..33823
                     /gene="rhaD"
                     /locus_tag="ACHKUN_000918"
     CDS             32999..33823
                     /gene="rhaD"
                     /locus_tag="ACHKUN_000918"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO6659227.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33895..35043
                     /gene="fucO"
                     /locus_tag="ACHKUN_000919"
     CDS             33895..35043
                     /gene="fucO"
                     /locus_tag="ACHKUN_000919"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO6659228.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            35040..35354
                     /gene="rhaM"
                     /locus_tag="ACHKUN_000920"
     CDS             35040..35354
                     /gene="rhaM"
                     /locus_tag="ACHKUN_000920"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO6659229.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35667..36113
                     /gene="frvA"
                     /locus_tag="ACHKUN_000921"
     CDS             35667..36113
                     /gene="frvA"
                     /locus_tag="ACHKUN_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO6659230.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36124..37575
                     /gene="frvB"
                     /locus_tag="ACHKUN_000922"
     CDS             36124..37575
                     /gene="frvB"
                     /locus_tag="ACHKUN_000922"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO6659231.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37565..38635
                     /gene="frvX"
                     /locus_tag="ACHKUN_000923"
     CDS             37565..38635
                     /gene="frvX"
                     /locus_tag="ACHKUN_000923"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6659232.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAKH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38635..40383
                     /gene="frvR"
                     /locus_tag="ACHKUN_000924"
     CDS             38635..40383
                     /gene="frvR"
                     /locus_tag="ACHKUN_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO6659233.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40433..>41430)
                     /gene="yiiG"
                     /locus_tag="ACHKUN_000925"
     CDS             complement(40433..>41430)
                     /gene="yiiG"
                     /locus_tag="ACHKUN_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO6659234.1"
                     /translation="ALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETPA
                     QPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIYG
                     ISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKD
                     DAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYS
                     LAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQ
                     LEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT        10059 a        10719 c        10728 g         9924 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcagcggat tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatgcg caccccggct gaaccctgga caatgctact gccatactgc
      181 gtcatgtcgc cctgacgcgc tgccggtttt ccgctcatcg taaaatcctt ttgaaataaa
      241 taagaaccag aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg
      301 aatttttcag aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt
      361 ttttcggaaa tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca
      421 acacggccac cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg
      481 cacacgtcga ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa
      541 gagcagctag cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct
      601 cggtttaagg ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt
      661 atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt
      721 atgcaaactg atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt
      781 caggatgatg ctatgcccgt agcccacgtt gccttgcccg ttccgcttcc tcgtaccttt
      841 gactatctgc tgccagaagg catgacggtt aaagctgggt gtcgcgtgcg cgtgccgttt
      901 ggcaaacagc aggagcgcat cgggattgtg gtatcagtta gcgatgccag cgaactgccg
      961 ctcaatgagc taaaagcggt agtcgaagtg ctggacgttg agccggtatt tactcactcc
     1021 gtctggcggt tactgctctg ggcggcagat tactatcatc atccgattgg cgatgtgctg
     1081 tttcatgcct tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg
     1141 tactggtttg ccactgaaca aggtcaggcg gtggatctta acagcctgaa acgttcgcca
     1201 aagcaacaac aggcgctggc ggcgttacgg caaggcaaaa tctggcgcga ccaggtcgcg
     1261 gaacttgaat ttaatgatgc cgcgttgcag gcgctgcgca aaaaaggtct gtgtgattta
     1321 gcaagtgaaa caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg
     1381 ttgcgattga ataccgaaca agccaccgcc gttggcgcaa ttcatagcgc ggcagatact
     1441 ttttctgcct ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt
     1501 gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc
     1561 ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta atgcccccgt ggaagttctg
     1621 cattccggcc tgaacgacag cgagcggctt tcggcgtggt tgaaagcgaa aaatggcgag
     1681 gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg
     1741 attgtcattg atgaagagca cgacagctcc tacaagcagc aggaaggctg gcgctatcat
     1801 gcccgcgacc tggcggtgta tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc
     1861 gcaacgcccg cgctggaaac gttatgcaac gtccagcaga aaaaataccg cctgctgcgc
     1921 ctgacccgtc gggcagggaa tgcgcgtccg gcaattcaac atgtgctgga tttaaaaggt
     1981 cagaaggtgc aggcaggtct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag
     2041 gccaacaatc aggttatcct ctttcttaat cgtcggggtt ttgcgcctgc gctgctgtgc
     2101 cacgactgcg gctggatagc cgaatgccca cgttgcgatc actactacac gctgcatcag
     2161 gcgcaacagc atttgcgctg tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc
     2221 ccttcctgcg gttccacgca cctggtcccc gtggggctgg gcaccgaaca gcttgaacag
     2281 acgcttgcgc cgatgttccc cggcgtgccc atttcacgta tcgaccgcga taccaccagc
     2341 cgcaaagggg cgctggaaca gcaactggcg gaagtgcatc gcggcggcgc gaggatcttg
     2401 attggcacgc aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta
     2461 ctggatgtgg acggcgcgct gttttctgcc gattttcgct cggcagaacg tttcgcccag
     2521 ctttacaccc aggtcgccgg tcgtgccggg cgtgcgggta aacagggcga agtggtgctg
     2581 caaacgcacc atccggaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc
     2641 tttgccgaac aggcgctggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat
     2701 gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg
     2761 cgtaatctga tcctttccag cccgctggca gacgacaaac tgtgggttct cggcccggtt
     2821 ccggctctgg cacctaagcg tggtggccgc tggcgctggc agatcttgtt gcagcaccct
     2881 tcccgcgtgc gcttgcaaca catcattagt ggtacgctgg cgcttattaa tacaataccg
     2941 gattcccgca aggtgaaatg ggtgttggat gtcgatccca tcgaaggcta atgcgcaaac
     3001 gttgcgaggc ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc
     3061 gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg
     3121 cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca
     3181 tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga
     3241 atcccgataa agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg
     3301 gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg
     3361 tgattgtccc ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta
     3421 cggcggcaaa tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg
     3481 aaaaaacctt tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt
     3541 caaggctgcc gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg
     3601 cgaacgaatt cgcaccggaa ctggagctac ctacagttca tatcgacaat ctgaccgccg
     3661 catttgatgc agtaaattat ctatatgagc aagggcataa acggattggc tgtatagccg
     3721 gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc
     3781 gctgcggcat tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg
     3841 gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc
     3901 atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc
     3961 cggaagacct ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc
     4021 cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc
     4081 ttgatcaaat gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta
     4141 tcatccgggg atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg
     4201 tcaaaggccc gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg
     4261 atagtggcac aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag
     4321 agcacctcac ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt
     4381 gctgccgccg ttcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa
     4441 gaagagtccg agacgctgca aagccagaaa gtgaccggta acggactacc accaaaacca
     4501 gaagaacgct ggcgctacat taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc
     4561 acagaacctt ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt
     4621 cagcttcttg aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt
     4681 ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg
     4741 cagcaattag cggagcagca acgcctggct cagcagtcgc gtacgactga acaaagctgg
     4801 cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa
     4861 ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg
     4921 cagtcgaagc cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg
     4981 gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa
     5041 caggcagaga cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc
     5101 aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc
     5161 accctcaatc ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt
     5221 tgaaaccctc aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg
     5281 tttgtactct gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc
     5341 gtaacggcca tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga
     5401 aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg
     5461 gcggtactgc ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc
     5521 agggccatct ggtcaaagcc gccgttgagc tagcgaaaga ctggcgtacc gatcgcatgc
     5581 tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg
     5641 gtaacggtga cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt
     5701 acgcccaggc tgcggcgcgt gcactgttag aaaatactga actgagtgct cgtgaaattg
     5761 ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca
     5821 tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa
     5881 tcgtcagcga actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga
     5941 ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga
     6001 ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc
     6061 gtctggctaa gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag
     6121 taggctacgt cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga
     6181 aaatggtacg cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag
     6241 aacgtattct cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc
     6301 agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg
     6361 atgacaaaga aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc
     6421 ctccaggcat ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc
     6481 agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag
     6541 aagcggcgaa actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc
     6601 agcacgggat cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg
     6661 gtccggatgt ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca
     6721 ccgtttccac caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg
     6781 cgttccagat tgcgaaaccg tctgacctaa tcccggaact gcaaggtcgt ctgccaatcc
     6841 gcgttgaact gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg
     6901 cctctatcac cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta
     6961 ccgactccgg tattaagcgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa
     7021 acatcggtgc tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg
     7081 acgccagcga tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc
     7141 tggatgcgtt ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca
     7201 ttttcatcat tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg
     7261 aacaacaaat tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc
     7321 tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc
     7381 cgctggtcgc cctgctggca ctaattaccg ccgggctatt acagatcctt tctaacctcg
     7441 ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac
     7501 gcggcatgca aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg
     7561 tcgtgctcat ctgtctctcc gggttggcac tggttgcagt ggcatgccat acgctggccg
     7621 attttgtcgg tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg
     7681 tgggcaatcg tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg
     7741 gctggttgag tgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga
     7801 tccttccggc gaccgcatgc ggcctgctgg caacggcagt actgaatatt aataacctgc
     7861 gtgatatcaa tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag
     7921 tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc gctggtgtgt ctggcgctgt
     7981 ttaatctctt ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac
     8041 tggtgaagca agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc
     8101 tggaacgtac tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc
     8161 taagccagtg ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag
     8221 cccacatagc gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata
     8281 cgatacttcc gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt
     8341 ctccaacttt ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga
     8401 ggacaacggg ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt
     8461 cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt
     8521 acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg cgtcaggtag atgacctgga
     8581 agagttggat atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg
     8641 cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtc accttcttct ccggcgacca
     8701 tctttatgcc gacaataccg ggattattct ttctgaagat ccgctggata ttgaatgata
     8761 gaaaaggcac cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa
     8821 ttacgcggaa gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac
     8881 gttcctgcca gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc
     8941 gctgatgctg ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt
     9001 cgatttccat ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt
     9061 tctcaaacac ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata
     9121 aaaatcctcg tcccgattac cggtgacgcc ttaataaata cgagcgcact ttagttagct
     9181 ccgattgtat gaagccgcgc catcgctgtc cagcggcacg ccttgcagat tacggtttgc
     9241 cacacttttc atccttctcc tggtgacata atccacatca atcgaaaatg ttaataaatt
     9301 tgttgcgcga atgatctaac aaacatgcat catgtacaat cagatggaat aaatggcgcg
     9361 ataacgctca ttttatgacg aagcacacac attttaagtt cgatatttct cgcttttgct
     9421 cgttaacgat aagtttacag catgcctaca agcatcgtgg aggtccgtga ctttcacgca
     9481 tacaacaaac attaactctt caggatccga ttatgagtca aacatcaacc ttgaaaggcc
     9541 agtgcattgc tgaattcctc ggtaccgggt tgttgatttt cttcggtgtg ggttgcgttg
     9601 cagcactaaa agtcgctggt gcgtcttttg gtcagtggga aatcagtgtc atttggggac
     9661 tgggggtggc aatggccatc tacctgaccg caggggtttc cggcgcgcat cttaatcccg
     9721 ctgttaccat tgcattgtgg ctgtttgcct gtttcgacaa gcgcaaagtt attcctttta
     9781 tcgtttcaca agttgccggc gctttctgtg ctgcggcttt agtttacggg ctttactaca
     9841 atttattttt cgacttcgag cagactcatc acattgttcg cggcagcgtt gaaagtgttg
     9901 atctggctgg cactttctct acttacccta atcctcatat caattttgtg caggctttcg
     9961 cagttgagat ggtgattacc gctattctga tggggctgat cctggcgtta acggatgatg
    10021 gcaacggtgt accacgcggc cctttggctc ccttgctgat tggtctactg attgcggtca
    10081 ttggcgcatc tatgggccca ttgacaggtt ttgccatgaa cccagcgcgt gacttcggtc
    10141 cgaaagtctt tgcctggctg gcgggctggg gcaatgtcgc ctttaccggc ggcagagaca
    10201 ttccttactt cctggtgccg cttttcggcc ctatcgttgg cgcgattgta ggtgcatttg
    10261 cctaccgcaa actgattggt cgccatttgc cttgcgatat ctgtgttgtg gaagaaaagg
    10321 aaaccacaac tccttcagaa caaaaagctt cgctgtaata tgactacggg acaattaaac
    10381 atgactgaaa aaaaatatat cgttgcgctc gaccagggca ccaccagctc ccgcgcggtc
    10441 gtaatggatc acgatgccaa tatcattagc gtgtcgcagc gcgaatttga gcaaatctac
    10501 ccaaaaccag gttgggtaga acacgaccca atggaaatct gggccaccca aagctccacg
    10561 ctggtagaag tgctggcgaa agccgatatc agttccgatc aaattgcagc tatcggtatt
    10621 acgaaccagc gtgaaaccac tattgtctgg gaaaaagaaa ccggcaagcc tatctataac
    10681 gccattgtct ggcagtgccg tcgtaccgca gaaatctgcg agcatttaaa acgtgacggt
    10741 ttagaagatt atatccgcag caataccggt ctggtgattg acccgtactt ttctggcacc
    10801 aaagtgaagt ggatcctcga ccatgtggaa ggctctcgcg agcgtgcacg tcgtggtgaa
    10861 ttgctgtttg gtacggttga tacgtggctt atctggaaaa tgactcaggg ccgtgtccat
    10921 gtgaccgatt acaccaacgc ctctcgtacc atgttgttca acatccatac cctggactgg
    10981 gacgacaaaa tgctggaagt gctggatatt ccgcgcgaga tgctgccaga agtgcgtcgt
    11041 tcttccgaag tatacggtca gactaacatt ggcggcaaag gcggcacgcg tattccaatc
    11101 tccgggatcg ccggtgacca gcaggccgcg ctgtttggtc agttgtgcgt gaaagaaggg
    11161 atggcgaaga acacctatgg cactggctgc tttatgctga tgaacactgg cgagaaagcg
    11221 gtgaaatcag aaaacggcct gctgaccacc atcgcctgcg gcccgactgg cgaagtgaac
    11281 tatgcgttgg aaggtgcggt gtttatggca ggcgcatcca ttcagtggct gcgcgatgag
    11341 atgaagttga ttaacgacgc ctacgattcc gaatatttcg ccaccaaagt gcaaaacacc
    11401 aatggtgtgt atgtggttcc ggcatttacc gggctgggtg cgccgtactg ggacccgtat
    11461 gcgcgcgggg cgattttcgg tctgactcgt ggggtgaacg ctaaccacat tatacgcgcg
    11521 acgctggagt ctattgctta tcagacgcgt gacgtgctgg aagcgatgca ggccgactct
    11581 ggtatccgtc tgcacgccct gcgcgtggat ggtggcgcag tagcaaacaa tttcctgatg
    11641 cagttccagt ccgatattct cggcacccgc gttgagcgcc cggaagtgcg cgaagtcacc
    11701 gcattgggtg cggcctatct cgcaggcctg gcggttggct tctggcagaa cctcgacgag
    11761 ctgcaagaga aagcggtgat tgagcgcgag ttccgtccag gcatcgaaac cactgagcgt
    11821 aattaccgtt acgcaggctg gaaaaaagcg gttaaacgcg cgatggcgtg ggaagaacac
    11881 gacgaataat gtaaatgccg aatgaagcgt ttatgccgca tccggtagtc ccgaaacgtg
    11941 cgggggcaac cccgcacaca tcaataatcc ctcccttccc ctgtgctaca cttcgcgcca
    12001 ttccttactg cttagagttt gctatgagac gagaacttgc catcgaattt tcccgcgtca
    12061 ccgaatcagc ggcgctggct ggctacaaat ggttaggacg cggcgataaa aacaccgcgg
    12121 acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt gacggcacca
    12181 tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct ctacattggt gaaaaagtcg
    12241 gtactggtcg cggcgacgcg gtagatattg ctgttgatcc gattgaaggc acgcgcatga
    12301 cggcgatggg ccaggctaac gcgctggcgg tgctggcagt aggcgataaa ggctgcttcc
    12361 tcaatgcgcc ggatatgtat atggagaagc tgattgtcgg gccgggagcc aaaggcacca
    12421 ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt agcggcggcg ctcggcaaac
    12481 cgttgagcga actgacggta acgattctgg ctaaaccacg ccacgatgcc gttatcgctg
    12541 aaatgcagca actcggcgta cgcgtatttg ctattccgga tggcgacgtt gcggcctcaa
    12601 ttctcacctg tatgccagac agcgaagttg acgtgctgta cggtattggt ggcgcgccgg
    12661 aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc
    12721 tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc gagcaggagc
    12781 tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt attgcgcctg ggcgatatgg
    12841 cgcgcagcga taacgtcatc ttctctgcca ccggtattac caaaggcgat ctgctggaag
    12901 gcattagccg caaaggcaat atcgcgacta ccgaaacgct gctgatccgc ggcaagtcac
    12961 gcaccattcg ccgcattcag tccatccact atctggatcg caaagacccg gaaatgcagg
    13021 tgcacatcct ctgattgatt tgatcgattg agccttccag tccttcggga ctggaatttt
    13081 tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt
    13141 gggtaacagg caaagtcact aaagtgcaga actggaccga cgccctgttt agtctcaccg
    13201 ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac caagcttggc cttgaaatcg
    13261 acggcgaacg cgtccagcgc gcctactcct atgttaactc gcccgataat cccgatctgg
    13321 agttttacct ggtcaccgtc cccgatggca aattaagccc acgattggcg gcactgaaac
    13381 caggcgatga agtgcaggtg gttagcgaag cagctggctt ctttgttctg gatgaagtac
    13441 cggactgcga aacgctatgg atgctggcaa ccggtacagc gattggccct tatttatcga
    13501 ttctgcaact aggcaaagat ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac
    13561 gttatgccgc cgacttaagc tatttgccac tgatgcaaga actggaaaaa cgctacgaag
    13621 ggaaactgcg cattcagacg gtggtcagtc gggaaacggc agcggggtcg ctcaccgggc
    13681 ggataccggc gttaattgaa agtggggaac tggaaagcgc gattggcctg ccgatgaata
    13741 aagaaaccag ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat acacaacagt
    13801 tgctgaaaga gacccggcag atgacgaaac atttacgtcg ccgaccgggc catatgacag
    13861 cggagcatta ctggtaagcg gttacttatc gataaacggc acgatgagca aatccgcact
    13921 catcttattg atcatcccgc gatatgccgg catcaaacgg ttgataaatg agtgatgatg
    13981 accacagaca aggaggtcgc actgctcttt ttgcataatt tccagcagtg tttccggcat
    14041 ttctccgcgt tcaatacgca gttttgtctt cggccattga atatttttcg tcagtttata
    14101 cagcttgtta tccgacttat tcttcaacaa ttgaagaata tcttctgttg cagggaagta
    14161 gatacccgga tacaactcgc ttaagccatc atcaatatga attaacgtca ggtgagcgtc
    14221 attatgtctg gcgagctcca gagctttatt caccagtaag gcatcttctt cattcccgga
    14281 aattgccacg ccaatgtgtt tataagccat gtttaactcc ttctaaagca actccatcaa
    14341 gctaacgaac gcagtgataa ctcaaaaata atcatctcgg cctggcatga gaaagtgaag
    14401 gccgcatcaa gctcaacttg cccctccaga tccttaatct tgtaggccac gctggcccct
    14461 tcatctgcag aggatgcagc ggcggcaagc tctttcagtt tggtcataaa agactctgct
    14521 tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc
    14581 gtaccgatat caatcgcaca tcctttagta ctgcatttat ctacgacatc tttcataggg
    14641 cacctctgta tattcgcctc tctgtcgatt acgacttaag aataatgcgc cgttaaggga
    14701 agaacaaaca agtaatgatc ttatggatta ccattttttt tgatattgct taaggtgaag
    14761 agttcagaag gattttccgg gtattgtcat ttgcatcgtg gaaataacaa tcccggaaaa
    14821 aaatagcagt aagagatata ttaaaggtta attacttgat ttattgtcgg ctttatactt
    14881 cacatcctga gtatctttac catatttatt ttcgccttgt gtgccaacaa acgcaccaag
    14941 atcgataagc atcatcacca gaatcaacgt cgggacaaaa cgccccaccg cccattgcca
    15001 gacacccggt aaaatcgccc agttacccgc cagcagcatc cacgccacaa tcatcagaaa
    15061 tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta acagttactg ccgctgttgg
    15121 ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata tcgagtaaat ttttacccgc
    15181 cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct atccaaatcc agaaatcacg
    15241 gcgtccaata cgccctttaa atgagaataa ccattgctgt atggtcatgt aagttccttg
    15301 atggttgtct tttccaggat tctacccttt tgacaagggc gacagatatc gttttaatcg
    15361 gagccagtca taacaaaagg tactgtcaat gaagccaggg tgtacgctgt tttttctctt
    15421 atgttctgca ttaaccgtta caacaacggc gcatgcacaa acaccagata cggcaacgac
    15481 cgcgccttat ctgctggctg gagcccctac tttcgatctc tccatcagcc agtttcgaga
    15541 agactttaac agccagaatc ctagcctgcc actgaacgaa tttcgtgcca tcgacagcag
    15601 tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt aacgagaact tgtatgcttc
    15661 tacagcgctg gagcgcggta ccttaaaaat caaaagcatt caaatgacct ggctacccat
    15721 ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag gaatacatgg cagcagtgat
    15781 ccgcacactc accccattaa tgaccaaaac acaaagccag aaaaaactgc agtcgctact
    15841 aacggcgggg aaaaacaaac gttattacac cgagacagaa ggtgcactgc gttatgttgt
    15901 cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa ccgattaagc tggcgctatc
    15961 tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag cctttgtgcc gatgaatctc
    16021 tatactgttt cacagacctg ctgccctgcg gggcggccat cttcctttat tcgcttataa
    16081 gcgtggagaa ttaaaatgcg acatccttta gtgatgggta actggaaact gaacggcagc
    16141 cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag agctggcagg tgttgctggc
    16201 tgtgcggttg caatcgcacc accggaaatg tacatcgata tggcgaagcg cgaagctgaa
    16261 ggcagccaca tcatgctggg tgcgcaaaac gtggacctga acctgtccgg cgcattcacc
    16321 ggtgaaactt ctgccgctat gctgaaagac atcggcgctc agtacatcat catcggccac
    16381 tctgaacgtc gtacttacca caaagagtct gacgaactga ttgcgaaaaa attcgcggtg
    16441 ctgaaagagc agggcctgac tccggttctg tgcatcggtg aaaccgaagc tgaaaacgaa
    16501 gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg cggtactgaa aactcagggt
    16561 gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg tatgggcaat cggtactggc
    16621 aaatctgcaa ctccggctca ggcacaggct gttcacaaat tcatccgtga ccacatcgct
    16681 aaagttgacg ctaacatcgc tgaacaagtg atcattcagt acggcggctc tgtaaacgcg
    16741 tctaacgctg cagaactgtt tgctcagcca gatatcgacg gcgcgctggt tggcggtgct
    16801 tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg cagaagcggc taaacaggct
    16861 taagtctgac aggtgccgga tttcatatcc ggcacttact ttccttaact cctcgcctta
    16921 acgcaaaatc tcacactcat gatcctggat ttcctcggct gaagcacggt tcagcgtcag
    16981 tagatttcgt tgtgtcgcca gcaacacaaa cgaattatca ctctgccgta ccatcgccag
    17041 cccgtagctt cccatatgtt cccgcgcctc aggcacttct tctgcaagca tcataaatgg
    17101 gctgcgttgt accagttcgc tttccgttac ccgacgcgcc aggtattcat gcccgcgcaa
    17161 accacctggc agtggcaacc agcggctgct gatgttcgcc agatttttat ccagttgttc
    17221 gcgcacatca ggacgaatac aagagatatg aatatgaaaa tggttttgcg tacgcccggt
    17281 gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc ggctggccgt attttttgct
    17341 cataaaatca cgcgcctgcc aggccaacca aaagaagttc ggcgttgaag gatcggtcaa
    17401 caaaggactt tcagtaccgt taatacgata cgttggcatc aacagatatt gcagtggacc
    17461 attaagatct tttaaaacca cgtatccggc attgggtttg acttccgcac atggcgaagg
    17521 attttgattt tgctgctgat tgggcaaaca ttcctcaagg acaatcttac gtaatgtatc
    17581 cgactcttca ccggttaatt tccagtaacc aataccggca gccacaacgg cgataactat
    17641 catcaccaaa aaaagaagac ccgctttttt catctttttt tccctgtacc tcaaagagat
    17701 gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt cgcgagaaac caccgggttt
    17761 gataattatc ctggggaaat cagcgtttgc tgatctgatc gaacgtaccg ccgttagcaa
    17821 aatgctcttt ttgcgctttc gtccagccgc cgaactcttc atcaatggtg aataacttca
    17881 gctttggaaa cgcattttcg tactttttcg ctacttcagc gtcgcgcgga cggtagtagt
    17941 ttttcgcggc aatttcctga ccttctggcg agtagagata tttcaggtag gcttccgcca
    18001 cctctttggt gccttttttc tcgaccactt tatcgaccac cgacacggtt ggctctgcga
    18061 ggatagactc actcggcgtg acgatttcga atttatcttt ccccagttca ttcgctacca
    18121 gcagagcttc gttttcccag gcgatcagta catcgccaat cccgcgctcg acaaaggtgt
    18181 tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt tttatacagt gcccgaacaa
    18241 aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag cgcgtagccc caggcagcca
    18301 ggtagttcca gcgtgcgcca ccagagcttt tcggattagg cgtgatcacc gaaacacccg
    18361 gtttaatcag atcgttccag tcatggatct gcttcggatt gcccttacgc accaggaaaa
    18421 caatggtgga agtgtacggt gcggagttat ccggcagacg tttgatccac tctttatcaa
    18481 tccgcccgcg ttccgcaatc gcgtccacgt cataggccag agccagcgtg acaacatcag
    18541 cttcaatacc gttgattacc gacgtcgctt gtttacctga accaccgtgt gactgacgaa
    18601 tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct gaatgccttg ttgtactgtt
    18661 cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag ctgaatatcc tttgccataa
    18721 cgttggttgc cgccagcaaa aatgttaacc ctacgcccca cttgttcatc gcccgactct
    18781 cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt aaccaaacat taaagaataa
    18841 aaaaagattg gctataactt gcgggtatat gttgggggtt aaaaaggcgg aagaaatccg
    18901 cctcatattg ctgacaaagt gcgctttgtc catgccggat gcggcgcgaa cgccttatcc
    18961 ggcctacaaa agtttgcaaa ttcaataaat tgcagaattc atgtaggcct gataagcgaa
    19021 gcgcatcagg catttttgct tctgtcatcg gtttcagggt aaaggaatct gcctttttcc
    19081 gaaatcatta atacagtttt ttcgcgcagt ccagccagtc acctttgaac ggacgcttca
    19141 tgttttcgat agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgg atacctacac
    19201 aacgaccgcc gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca
    19261 gaatacggtc gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc
    19321 gggtttcacg accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat
    19381 gttcggtaat cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt
    19441 ttaccaggtc ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg
    19501 caatggccgc agccaacgtc agatcgccac aataacggcc catcacttcc accacggaaa
    19561 tacgctggtg agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca
    19621 gcgcagtgaa gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg
    19681 gcagaccgat gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt
    19741 caccgccgat aaccaccagc gcgtcgatcc cacgtttttt caggttttcg atagccacgg
    19801 cgcggatgtt ctcgtcgcgg aattccggga agcgcgcaga accgaggaac gtaccgccac
    19861 ggttgatcat gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac
    19921 ccagatagcc gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa
    19981 ccccgcgaat tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt
    20041 tcttaatcat gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct
    20101 tcttgcacat cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga
    20161 attgattcag gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct
    20221 tttatactga ttatgaaagc atagaccgtt taccctccct gggtacgacg gaacaggggt
    20281 cctgatggat aattacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat
    20341 ccgccaccat atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa
    20401 taaagcgggt cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc
    20461 aggaagtcac gatatcaata atttcttgcc gttcctcatc aggcaatgcg cgatccagta
    20521 atgactgtac cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc
    20581 ccaatgcaaa cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca
    20641 gaattgcgcc gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct
    20701 ggctttgcgt ccggcgcacc acccaacgct gaaacgagac aaggataatc gtacaaatta
    20761 gcgccacaat tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatatca
    20821 gatgttgaat acccgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg
    20881 ccagcgccgc gagggactct gctttaccgt gaccaaacga gtgattatcg tcggcaggtt
    20941 gcagggaata tcgcaccacc agtaaattcg tcaacgacgc gccgatatcc accagcgaat
    21001 ccaccagcgc ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca
    21061 gcaatagcaa cgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat
    21121 aagattgatt cataaatact cccgctatca actgacgcta gtataacgga agcaaatcat
    21181 ctgcaatgca ttaagcagca ggcaaattga ggataaaaaa aacccccaca tcatgtgggg
    21241 gaagacaggg atggtgtcta tggcaaggaa aacagggttt actactggga acgtgagttg
    21301 ctactactca atagcttcaa cgatgaactt ttttgccatt gcgtcacgtc acgcaactgc
    21361 tccattcgtt gttgatgttt ctcgtttaaa accgcttgct gctccggcgt taacaggcga
    21421 tacatttggt tgcggacttt tgccatctca acctgacgag caatttgctc attcgccatt
    21481 ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt ctgcggtgac aaggcgatgc
    21541 attgtctcca gttcgctaac attaacagga ggctgttcgt gccgggcctg ttgcataaga
    21601 tctcgcatct gctgacgctg atgttcggtt aaacttatgc cgtcgaacat atggctctgc
    21661 gtactgcgct gcgtaagttc ttcacccgga tgccagttat cgcctgaacc gacttcagca
    21721 gcgtggctta atgaactgac tgccagcgtt gaggccatga cggcagcggt aactatgcgc
    21781 atcatttgct cccaaaatct ttctgtcgcg attcaacgat agagagttta cgattcaggc
    21841 tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca tggattagcg acgtctgatg
    21901 acgtaatttc tgcctcggag gtatttaaac aatgaataaa atcctgttag ttgatgatga
    21961 ccgagagctg acttccctat taaaggagct gctcgagatg gaaggcttca acgtgattgt
    22021 tgcccacgat ggggaacagg cgcttgatct tctggacgac agcattgatt tacttttgct
    22081 tgacgtaatg atgccgaaga aaaatggtat cgacacatta aaagcacttc gccagacaca
    22141 ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa cttgatcgcg ttctcggcct
    22201 tgagctgggc gcagatgact atctcccgaa accgtttaat gatcgtgagc tggtggcacg
    22261 tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa cagcaaaaca acgacaacgg
    22321 ttcaccgaca ctggaagttg atgccttagt gctgaatcca ggccgtcagg aagccagctt
    22381 cgacgggcaa acgctggagt taaccggtac tgagtttacc ctgctctatt tgctggcaca
    22441 gcatctgggt caggtggttt cccgtgaaca tttaagccag gaagtgttgg gcaaacgcct
    22501 gacgcctttc gaccgcgcta ttgatatgca catttccaac ctgcgtcgta aactgccgga
    22561 tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt cgcggctatc tgatggtttc
    22621 tgcttcatga taggcagctt aaccgcgcgc atcttcgcca tcttctggct gacgctggcg
    22681 ctggtgttga tgttggtttt gatgttaccc aagctcgatt cacgccagat gaccgagctt
    22741 ctggatagcg aacagcgtca ggggctgatg attgagcagc atgtcgaagc ggagctggcg
    22801 aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt tccgggcgat tgataagtgg
    22861 gcaccgccag gacagcgttt gttattggtg accaccgaag gccgcgtgat cggcgctgaa
    22921 cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg ccgataacgc cgatcatccg
    22981 cagaagaaaa agtatggccg cgtggaactg gtcggtccgt tctccgtgcg tgatggcgaa
    23041 gataattacc aactttatct gattcgtccg gccagcagtt ctcaatccga tttcattaac
    23101 ttactgtttg accgcccgct attactgctg attgtcacca tgttggtcag tacgccgctg
    23161 ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta agctgaaaaa cgctgccgat
    23221 gaagttgccc agggaaactt acgccagcac ccggaactgg aagcggggcc acaggaattc
    23281 cttgccgcag gtgccagttt taaccagatg gtcaccgcgc tggagcgcat gatgacctct
    23341 cagcagcgtc tgctttctga tatctctcac gagctgcgca ccccgctgac gcgtctgcaa
    23401 ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca aggaactcga gcgtattgaa
    23461 accgaagcgc aacgtctgga cagcatgatc aacgatctgt tggtgatgtc acgtaatcag
    23521 caaaaaaacg cgctggttag cgaaaccatc aaagccaacc agttgtggag tgaagtgctg
    23581 gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt tgacagttaa cttcccgcct
    23641 gggccgtggc cgctgtacgg caatccgaac gccctggaaa gtgcgctgga aaacattgtt
    23701 cgtaatgctc tgcgttattc ccatacgaag attgaagtgg gctttgcggt agataaagac
    23761 ggtatcacca ttacggtgga cgacgatggt cctggcgtta gcccggaaga tcgcgaacag
    23821 attttccgtc cgttctatcg gaccgatgaa gcacgcgatc gtgaatctgg cggtacagga
    23881 ttggggctgg cgattgttga aaccgccatt cagcagcatc gtggctgggt gaaggcagaa
    23941 gacagcccgc tgggcggttt acggctggtg atttggttgc cgctgtataa gcggagttaa
    24001 actccgcatt tgtaggcagg ataaggcgtt tacgccgcat ccggcatttg agcaggataa
    24061 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    24121 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    24181 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    24241 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    24301 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    24361 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    24421 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    24481 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    24541 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    24601 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    24661 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    24721 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    24781 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    24841 ccccattaga ccatatttat tttatgtcag gcgtgctgtt ttcgcggtaa gtatccaggc
    24901 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    24961 aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg
    25021 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca
    25081 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag
    25141 gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac
    25201 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt actgccacac
    25261 ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca
    25321 gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt
    25381 cagggtcaag gttccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa
    25441 catgcggttc aaacgaggca atccctgcag tgcccagaat aatcatcgtc atgagcggcc
    25501 cggactccaa cgacatcaac acaaatgccc cagcttcttc ttttgtgccg tactgctgca
    25561 tgatggaagc gtaaagtccg ccattggtca tatccatcgc cgccaccagc gccagcgttg
    25621 aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    25681 ccgcaaccac ccacgcgacg gcaatttttg ttaccaccag cgtaccggat ttacgcaata
    25741 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    25801 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    25861 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga
    25921 ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct
    25981 gatgcgtcat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta
    26041 atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt atcggatcat
    26101 tgtttttatt ttgaaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
    26161 aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
    26221 gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt aatgtagtcc
    26281 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
    26341 gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta
    26401 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
    26461 gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
    26521 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
    26581 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
    26641 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
    26701 agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
    26761 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
    26821 tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt
    26881 ggttcattat agttaattaa atgatattga aaatgattat caatgccgta cttttcgtaa
    26941 gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg
    27001 attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt
    27061 acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa
    27121 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    27181 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat
    27241 tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc
    27301 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    27361 ggggatcggt aatatcaact acggcctgac catgcgttat ctcggcatgt cgatgggaat
    27421 tggcatcgcc attggcatta cgttgattgt cggtacactg atgacgccaa ttatcaacgg
    27481 caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttgctcg gcgttctggt
    27541 ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg
    27601 cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtta tgtgcggcat
    27661 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    27721 cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg
    27781 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    27841 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc
    27901 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctggggcc acgcccgcat
    27961 tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg
    28021 cggtatcgtc ggactggtgc tgaaagagtg gagcaatgca ggacgccgtc cggtaacggt
    28081 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    28141 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    28201 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    28261 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag
    28321 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact
    28381 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag
    28441 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    28501 gtaacgatgg cgattcagca acatcgccaa ctgcccgaac agcaactctg ccatttcgtt
    28561 agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    28621 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    28681 aatcgccccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg
    28741 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tgtaaaagag
    28801 atcgccacgg gtaatgcgat aagggcgatc gttgagtaca tgcaggccat taccgcgcca
    28861 gacaatcacc agctcacaaa aatcatgtgt atgttcagca aagacatgtt gcggataacg
    28921 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    28981 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    29041 tacaaatacg ttgagaagat tcgcgttatt gcagaaaacc atcccgtccc tgacgaatat
    29101 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    29161 atccgcaatg ataggcgatg tcagtaacgc tggcctcact gtggcgtagc agatgtcggg
    29221 ccttcatcag tcgcaggcgg ttcaggtaac gctgaggcgt cagtcccgtt tgctgcttaa
    29281 gctgccgatg tagcgttcgc agtgaaagag aaaattgatc cgccacggca tcccaattca
    29341 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    29401 tttccaggtt cttctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaag
    29461 atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca
    29521 accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga cggatactgc
    29581 ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc
    29641 gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag atgccgatca
    29701 tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca
    29761 tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga
    29821 aaatccgcct gagggagccg gggttctatc gccacggacg cgttaccaga cggaaaaaaa
    29881 tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta
    29941 atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat
    30001 ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaatgaa
    30061 gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt
    30121 cagtaacgag aaggtcacga attcaggcgc tttttagact ggtcgtaatg aaattcagca
    30181 ggatcacatt atgacctttc gcaattgtgt cgccgtcaat ctcggcgcat ccagtgggcg
    30241 cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg
    30301 ttttaaaaat gggctgcata gccagaacgg ttatgtcacc tgggatgtgg atagcctgga
    30361 aagtgccatt cgccttggct taaacaaagt gtgtgaggaa gggattcgta tcgatagcat
    30421 tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg
    30481 cctgcccgtt gcttatcgcg atagccgcac caatggccta atggcgcagg cacagcaaca
    30541 actcggcaaa cgcgatattt atcaacgtag cggcatccag tttctgccct tcaatacgct
    30601 ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc
    30661 tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact gggagtacac
    30721 caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg agtcgctact
    30781 ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat
    30841 tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga
    30901 taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt atctctcttc
    30961 tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct
    31021 ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat
    31081 tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg atctcccggc
    31141 gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc ccaatgacga
    31201 tcgctttatt aatcctgagg cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc
    31261 gcaaccgatc ccggaaagtg atgctgaact ggcgcgctgt attttcgaca gtctggcgct
    31321 gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt tctcgcaact
    31381 gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg ccgatgcctg
    31441 cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata tcggcatcca
    31501 gttaatgacg ctggatgaac tcaacaatgt ggataatttc cgtcaggtcg tcagcaccac
    31561 cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact atgtggcgca
    31621 gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact ggaacaggcc
    31681 tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga ggcgctgcgc
    31741 caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt
    31801 gaaaacccgg aaggttcgct gaccggtggg attcaagcta ctggtaatta tccgggcaaa
    31861 gcgcgtaatg ccagtgagct acgtaccgat ctggaacagg ctatgcggct gattccgggg
    31921 ccgaaacggc ttaatttaca tgccatctat ctggaatcag acacgccagt ctcgcgcgac
    31981 cagatcaaac ctgagcactt caaaaactgg gttgaatggg cgaaagccaa tcagctcggt
    32041 ctggatttta acccctcctg tttttcgcat ccgctaagcg ccgatggctt tacgctttcc
    32101 catgccgacg acagcattcg ccagttctgg attgatcact gcaaagccag ccgtcgcgtt
    32161 tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg gatcccggat
    32221 ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct ggcagcactg
    32281 gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt tgagagcaaa
    32341 ttgtttggca ttggcgcgga gagctacacg gttggctcca atgagtttta cctggggtat
    32401 gccaccagcc gccagacggc gctgtgcctg gacgccgggc acttccaccc gactgaagtg
    32461 atttccgaca agatttccgc cgccatgctg tatgtgccgc agttgctgct gcacgtcagt
    32521 cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac ccaggcaatt
    32581 gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct tgacttcttc
    32641 gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc
    32701 ctgctgcgtg cgttgctgga acctaccgcc gagctgcgca agttggaagc ggcgggcgat
    32761 tacactgcgc gtcaggcact gctggaagag cagaaatcgt tgccgtggca ggcggtatgg
    32821 gaaatgtatt gtcagcgtca cgatacccca gcgggtagcg aatggctgga gagcgtgcgg
    32881 gcttatgaga aagaaatttt gagtcagcgt gggtaaacac tgccagatgc ggcacgagcg
    32941 ccttatccgg cctacagatc gtgcatctga caacacccca tttacaggaa caccgaacat
    33001 gcaaaacatt actcagtcct ggtttgtcca gggaatgatc aaagccacca ccgacgcctg
    33061 gctgaaaggc tgggacgagc gcaacggcgg caacctgacg ctacgcctgg atgacgctga
    33121 catcgcaccg tatcacgaca atttccacca acaaccgcgt tatatcccgc tcagccaacc
    33181 catgccttta ctggcaaata caccgtttat cgtcaccggc tcgggcaaat tcttccgtaa
    33241 cgtccagctt gatcctgcgg ctaacttagg cgtcgtaaaa gtcgacagcg acggcgcggg
    33301 ctaccatatt ctctgggggt taaccaacga ggccgttcca acttctgaac ttccggcaca
    33361 ctttctctcc cattgcgaac gcattaaagc caccaacggt aaagatcggg tgatcatgca
    33421 ctgccatgcc accaacctga tcgccctcac ctatgtactg gaaaacgaca cggcggtctt
    33481 cactcgccaa ctgtgggaag gcagcaccga gtgcctggtg gtattcccgg atggcgttgg
    33541 cattctgccg tggatggtgc ccggcacgga cgaaatcggt caggcgaccg cacaagaaat
    33601 gcaaaaacat tcgctggtgt tgtggccctt ccacggtgtg ttcggttccg gaccgacact
    33661 ggatgaagcc tttggtttga tagataccgc agagaaatca gcggaggtgt tagtcaaaat
    33721 ctactcgatg ggcggtatga agcagaccat tacccgtgag gagctgattg cactcggtga
    33781 gcgttttggc gttacgccac tggccagtgc gctggcactt taatatccca acagatatcc
    33841 ggcggtgtgg gaaacgtcgc cggacagatg gatttgaact taaggagagt cattatgagt
    33901 tttatgttgg cattaccgaa aattagcctg catggtgctg gggcaattgg cgatatggta
    33961 aatctggttg cgcataaaca gtggggaaaa gcactgatcg ttaccgatgg gcaactggtt
    34021 aagttgggat tactggacag cctgtttatc gcactggatc aatacgaaat gtcttatcac
    34081 ttatttgatg aggtatttcc taaccccact gaagaactgg tgcaaaaagg ctacgctgca
    34141 tttcaggatg ccgcatgtga ctacattatt gctttcggtg gcggtagccc aattgatact
    34201 gcaaaagccg taaaaatcct taccgctaat cctgcgcctt ctacggcgta ctctggtgtt
    34261 ggtaaagtga caaatcccgg cgtaccgctg gtggcaatca atacgactgc cggaacagct
    34321 gctgagatga ccagcaatgc ggtgatcatc gacaccgcgc ggcaggtaaa agaggtcatt
    34381 atcgacccga atattattcc cgatattgcg gtggatgatg ccagtgtaat gctggagatc
    34441 ccggcctcgg ttaccgccgc cacgggtatg gatgcactga ctcacgcagt tgaagcttat
    34501 gtttctgttg gtgcacatcc attaaccgac gctaacgcac tggaagctat ccgtttaatt
    34561 acgctgtggc tgccaaaagc ggtagatgat ggtcataatc tggaagctcg tgaacaaatg
    34621 gcattcgccc agtacctggc gggtatggca tttaacagtg ctggtctggg ccttgttcat
    34681 gcacttgcgc atcagcccgg cgccacccac aacctgccgc atggtgtgtg caatgctatc
    34741 ttgttgccag ttattgaaaa ctttaaccgc ccgaatgcca tcgcacgctt tgcccgcatt
    34801 gctcaggcaa tgggtgtcga tacgcgtgat atgagtgaag aagcggcaag tactgaagca
    34861 attaacgcta tccgcgcatt aagccatcgc gtgggtattc cggcaggatt cagccagctt
    34921 ggcatcacca aagaagatat tgaaggctgg ttagataaag ccctggctga cccgtgtgtg
    34981 ccatgtaacc cgcgtaccgc cagccgcgac gaggtgcgcg agctgtatct ggaggcatta
    35041 tgatccgtaa ggcatttgtg atgcaggtta accccgacgc tcacgaagag tatcaacgtc
    35101 ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact
    35161 acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg
    35221 aagagcgctg gaatgcggtt gccagtactg atgtttgcca acgttggtgg aaatatatga
    35281 ccgacgtcat gcccgcgaac ccggataaca gcccggtgag tagcgaactg caagaagtgt
    35341 tttacctgcc gtaattgcct aatgtgccgg agacgcctcc ggtacaatat ttttaatttc
    35401 gatttgaaaa ataaacatat tacaccttca gttaatatat ctcggtaact tatatttccc
    35461 cgatgcattg tgagtaaatc acaaaaataa tgaataactc attaatgatt catgtggttt
    35521 atttaaataa ccattatgtg cattactccg caaatctggc ctttcactct gtctcaaatt
    35581 gtcattgttt accgacatat cacctctcat cacttaatcg ctccaccatt acaattgatt
    35641 aaaatatatt ttatggagtc cgattaatgg cagctcttac tgcaagctgt attgacctga
    35701 atattcaggg caatggcgct tattccattc tgaagcagtt ggcgacaatg gcgttacaaa
    35761 acggttttat caccgactca caccagttcc tgcaaaccct gctgctgcgc gaaaaaatgc
    35821 actctactgg atttggttcc ggcatcgccg tgccgcacgg taaaagcgcc tgcgttaaac
    35881 aaccgttcgt gttattcgcc cgcaaagcgc aggccattga ctggaaagcc agcgacggcg
    35941 aagacgtcaa ttgctggatc tgcctcggcg tgccgcaaag tggcgaagag gatcaggtca
    36001 aaatcatcgg cacactgtgt cgcaaaatta tccatcagga ttttatcaac caactgcgac
    36061 agggcgacgc cgaccaggta cttgctttgt taaatcaaac cctcagctca taaggaagtg
    36121 gcgatggagt catccttacg tattgtcgcg atcaccaact gccccgccgg gatcgctcac
    36181 acctacatgg tggcggaagc cctggaacag aaagcccgtt ctctcggcca taccataaaa
    36241 gtggaaactc aagggtccag cggcgttgaa aaccgcttat ccagcgaaga gattgccgct
    36301 gccgattacg tcattctagc taccgggcgt ggcctgagcg gtgatgaccg ggcgcggttt
    36361 gccgggaaga aagtttatga gattgccatc tcccaggcgc tgaaaaatat cgaccagatt
    36421 ttcagcgaat taccaacaaa ctcgcagctt tttgccgcag atagcggcgt gaagctcggc
    36481 aaacaggaag tgcaaagcgg cagcgtgatg agccacctga tggcgggcgt ctctgcggcg
    36541 ctgccatttg tcatcggcgg cggtattctg gtggcgcttg ccaacatgct ggtgcagttc
    36601 ggtttgccat ataccgatat gtcgaaaggt gcgccgtcgt ttacctgggt agttgaatcc
    36661 atcggttacc tcggcttcac ctttatgatc cccatcatgg gcgcttatat tgcctcgtcg
    36721 attgccgata aacccgcttt cgctccggca tttctggttt gctatctggc gaacgataaa
    36781 gccctgctcg gcacccagtc aggcgcgggc ttcctcggcg cggtggtgct ggggctggcg
    36841 attggctatt tcgtcttctg gttccgtaaa gtccgtcttg gcaaagcatt acaaccgctg
    36901 ctcggctcca tgcttatccc attcgttact ctgctggttt ttggcgtgct cacttactac
    36961 gttatcgggc cggtgatgtc cgacctcatg ggcgggctgc tccacttcct gaataccatt
    37021 cctccatcaa tgaagtttgc ggcagcgttt ctggtcggtg cgatgctggc gttcgatatg
    37081 ggcggcccca tcaataaaac cgcctggttc ttctgcttct cactgctgga aaaacacatt
    37141 tatgactggt acgccatcgt cggcgttgtc gcgctgatgc cgcccgtcgc ggcgggtctt
    37201 gcgacgttca ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc
    37261 attgtggtcg gtgccacagt cgcaaccgaa ccggctattc cttacgctct ggcagctccg
    37321 ttgccgatga ttaccgccaa tacgctggcg ggcggtatta ccggcgtgct ggtgatcgct
    37381 ttcggaatca aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg
    37441 tcaccggtag gttcgtttta tctggtgctg gcgattggcc tcgcgctgaa catttcattc
    37501 attatcgtcc tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt
    37561 gtccatgaac attgagttac tgcaacagtt gtgcgaagcc agcgccgtta gcggcgatga
    37621 acaggaagtt cgcgacattc tgataaacac tctggaacct tgcgtgaatg aaatcacctt
    37681 tgatgggctg ggcagctttg tcgcccgtaa gggaaataaa gggccgaaag ttgccgttgt
    37741 cgggcatatg gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg
    37801 tttcaccacc attggtggct ggtggaatca gtcgatgctc aaccaccggg taaccatacg
    37861 cacacacaag ggagtgaaaa tccctggtgt gattggttcc gtcgcgcctc atgcgttaac
    37921 ggaaaagcaa aagcaacagc cgcagtcatt tgatgagatg ttcattgata ttggcgcaaa
    37981 cagtcgcgat gaggtggaaa aacgcggtgt ggcgatgggc gattttatta gcccggaagc
    38041 caattttgcc tgctggggcg aagataaagt ggtcggcaag gcgttggata accgcatcgg
    38101 ctgcgcaatg atggctgagc tactacagac ggtgaataat cccgaaatta cgctgtatgg
    38161 cgttggcagt gtggaagaag aagttgggct acgcggggcg caaacctcgg ctaaacacat
    38221 taaaccggat gtggtgatcg tgctggacac cgctgtcgcg ggcgatgttc caggcattga
    38281 taacattaaa tatccgctga aactgggcaa cgggccgggg ttgatgctgt ttgacaagcg
    38341 ctacttcccc aaccagaaac tggtagcagc gttaaaaagc tgtgccacgc agaatgattt
    38401 accgctgcaa ttttccacca tgaaaaccgg tgcgacggat ggcgggcgct acaacgtgat
    38461 gggcggtggg cgtccggttg tcgcgctgtg tttgccaacg cgttatctgc acgccaacag
    38521 cggcatgatt tcaaaagccg attacgatgc tctgctcacg ctgatacggg gttttctgac
    38581 caccttaacc gcggaaaaag tcagcgcgtt tagccagttc cgtcaggtgg attaatgcta
    38641 aacgaacgcc agttaaagat tgtcgatctg ctggagcaac agccgcgcac gcctggcgag
    38701 ctggcgcaac agactggcgt ttctggcagg accatactgc gtgatattga ctatctcaac
    38761 tttaccctta acggcaaagc ccgcatttct gccagtggta gtgcgggcta tcagctggaa
    38821 atcttcgagc gccgcagctt ttttcagttg ctgcaaaagc acgataacga cgatcggctg
    38881 ctggcactgt tattactgaa tacctttact ccccgtgcgc aactcgcctc ggcgcttaat
    38941 ttgccggaaa cgtgggtcgc agagcgtctg ccccggttaa aacagcgtta tgaacgcact
    39001 tgctgcctgg ccagtcgccc cggtttgggc catttcattg atgagacaga agaaaaacgc
    39061 gttatattgc tggcgaactt gctgcgcaaa gatccgtttt taattccgct ggcgggcgta
    39121 acacgagaca accttcagca tttatccacg gcctgcgaca accaacaccg ctggccgctc
    39181 atgcagggtg attatctctc cagcctgatt ctggcgattt acgccctgcg taatcaactg
    39241 accgatgagt ggccgcaata tcccggtgac gagataaaac aaatcgttga acatagcggt
    39301 ctgtttcttg gtgataacgc tgtaagaacc ctgacaggtt tgatagagaa acagcatcag
    39361 caagcgcaga taatttctgc cgataatgtg cagcggttgc tgcaaagggt gccgggcatc
    39421 gcgtcattga atattattga tacgcagctg gttgagaaca ttaccgggca tttattacgt
    39481 tgccttgccg caccggtgtg gattgctgag caccgccaaa gcagcatgaa taacctgaaa
    39541 gccgcctggc ctgcggcctt tgatatgagt ctgcacttta ttacgctact gcgtgaacag
    39601 ctcgatattc cccttttcga cagcgatctg atcggtttgt attttgcctg tgcgctggaa
    39661 cggcatcaaa acgaacgcca gccgatcatt ttgctctcgg accagaacgc gattgccact
    39721 attaatcagc tcgccattga gcgcgatgtt ttacattgtc gggtgattat tgcccgtagc
    39781 ttaagcgaac ttgttgccat tcgcgaagag attgagccgt tattgatcat taacaacagc
    39841 cattatttac tggatgacgc ggtaaataat tacatcaccg taaaaaatat cattacggct
    39901 gccggtatcg aacaaataaa acattttctg gcgacggcat ttattcgcca acagccggag
    39961 cgttttttct ctgctcccgg aagttttcat tattcgaatg tacgcggtga aagctggcaa
    40021 catattaccc ggcaaatttg tgcgcaatta gtcgcacaac accatattac cgccgatgaa
    40081 gcacaacgca tcatcgcccg cgaaggcgaa ggtgaaaacc tgattgttaa tcgcctcgcc
    40141 atcccacatt gctggagcga acaggagcga cgttttcgtg gattttttat taccctcgcc
    40201 caaccagttg aggtgaataa cgaagtcatt aaccatgtct tgatcgcctg cgccgccgcc
    40261 gatgcgcgtc atgagctaaa aatatttagc tatctggcaa gcatattgtg tcagcatccg
    40321 gcggagatta ttgccgggtt aacaggatat gaggcattta tggagttact tcacaagggg
    40381 tgaactgtgt cggatgcgcc gcatccgaca ctgcatttat catcaaaaac gtttaacgca
    40441 ggctattata gtcatcaacc atttcgttgt actcgcgtaa tgctctcggg aaagagtctt
    40501 cgaccatcca gcttgagttt gcgtcctgaa gttgctcttt atcccagtca gagtacggga
    40561 ctttatctct gatgcggcga acgtattttt tagcctcgag ttgatactgg cctgccgaat
    40621 taataaacga gaaattcatg ccgcctttat ccgcttcttt ggcctgcgcc actagcgttt
    40681 ccagttcaga cactttcttc attgccgcat caacatcaaa cttctcttgc gatatcaggt
    40741 tattaatttg tttcgctgaa atcatgactg ccagagagta gtagtggaat gttttccctt
    40801 ctctttcttc aatatttttc agttccgcaa gctggcgctt atcgttaatt tcctgaatcg
    40861 ccgcatgata agattctgct acaggttcaa aggcttccag attttttaag aatgtctggt
    40921 gcagcgtttt ccctttcgca aacgcatcgt ctttataatt ttcctgggtg taatatttat
    40981 ccatttcgtt aatggtatta cccaacgcca cggcagcatc gatataactc accgccacgc
    41041 catcaattgg ttgcagcgca ggcgttaacg ccacagcact ttttacgcct ttttcacact
    41101 cagcgaggtt ggattcacta atgccgtaga tgccataaac agtacgctct tcaccggtag
    41161 gcccctgttt aaaatctttc agccagtcag cataacgcgc cagactgctc tgtacaggga
    41221 tctgtaactt gttatagcac ttgatataga catccatttt ttcgtcgaaa accgcttgtt
    41281 catcgaccac cggttgcgca ggtgtttcag gtttagcttc tgcttttgca acgggagctt
    41341 caggagctgg tgcggcaggt tgactattgg caggcggggg cgtttctgtt tcggcttttt
    41401 tatcatcaca acccgtcaga ccgagggcca
//
LOCUS       DAZFRJ010000019        39542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000019 DAZFRJ010000000
VERSION     DAZFRJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.019"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..100
                     /locus_tag="ACHKUN_000926"
     CDS             <1..100
                     /locus_tag="ACHKUN_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6659235.1"
                     /translation="MTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            111..947
                     /gene="eutJ"
                     /locus_tag="ACHKUN_000927"
     CDS             111..947
                     /gene="eutJ"
                     /locus_tag="ACHKUN_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO6659236.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            937..2124
                     /gene="eutG"
                     /locus_tag="ACHKUN_000928"
     CDS             937..2124
                     /gene="eutG"
                     /locus_tag="ACHKUN_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO6659237.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2241..3467
                     /gene="eutH"
                     /locus_tag="ACHKUN_000929"
     CDS             2241..3467
                     /gene="eutH"
                     /locus_tag="ACHKUN_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO6659238.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3464..4867
                     /gene="eutA"
                     /locus_tag="ACHKUN_000930"
     CDS             3464..4867
                     /gene="eutA"
                     /locus_tag="ACHKUN_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO6659239.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4879..6240
                     /gene="eutB"
                     /locus_tag="ACHKUN_000931"
     CDS             4879..6240
                     /gene="eutB"
                     /locus_tag="ACHKUN_000931"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO6659240.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6261..7148
                     /gene="eutC"
                     /locus_tag="ACHKUN_000932"
     CDS             6261..7148
                     /gene="eutC"
                     /locus_tag="ACHKUN_000932"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO6659241.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7158..7817
                     /gene="eutL"
                     /locus_tag="ACHKUN_000933"
     CDS             7158..7817
                     /gene="eutL"
                     /locus_tag="ACHKUN_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO6659242.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7830..8330
                     /gene="eutK"
                     /locus_tag="ACHKUN_000934"
     CDS             7830..8330
                     /gene="eutK"
                     /locus_tag="ACHKUN_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO6659243.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8376..9428
                     /gene="eutR"
                     /locus_tag="ACHKUN_000935"
     CDS             8376..9428
                     /gene="eutR"
                     /locus_tag="ACHKUN_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO6659244.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9434..10333)
                     /gene="hemF"
                     /locus_tag="ACHKUN_000936"
     CDS             complement(9434..10333)
                     /gene="hemF"
                     /locus_tag="ACHKUN_000936"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO6659245.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERECNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10337..11206)
                     /gene="amiA"
                     /locus_tag="ACHKUN_000937"
     CDS             complement(10337..11206)
                     /gene="amiA"
                     /locus_tag="ACHKUN_000937"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO6659246.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11420..11845
                     /gene="ypeA"
                     /locus_tag="ACHKUN_000938"
     CDS             11420..11845
                     /gene="ypeA"
                     /locus_tag="ACHKUN_000938"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO6659247.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11832..12281
                     /gene="yfeZ"
                     /locus_tag="ACHKUN_000939"
     CDS             11832..12281
                     /gene="yfeZ"
                     /locus_tag="ACHKUN_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO6659248.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            12342..12917
                     /gene="yfeY"
                     /locus_tag="ACHKUN_000940"
     CDS             12342..12917
                     /gene="yfeY"
                     /locus_tag="ACHKUN_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO6659249.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13013..13912
                     /gene="yfeX"
                     /locus_tag="ACHKUN_000941"
     CDS             13013..13912
                     /gene="yfeX"
                     /locus_tag="ACHKUN_000941"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO6659250.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13970..15274)
                     /gene="pbp4b"
                     /locus_tag="ACHKUN_000942"
     CDS             complement(13970..15274)
                     /gene="pbp4b"
                     /locus_tag="ACHKUN_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO6659251.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(15279..16703)
                     /gene="murP"
                     /locus_tag="ACHKUN_000943"
     CDS             complement(15279..16703)
                     /gene="murP"
                     /locus_tag="ACHKUN_000943"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO6659252.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16707..17603)
                     /gene="murQ"
                     /locus_tag="ACHKUN_000944"
     CDS             complement(16707..17603)
                     /gene="murQ"
                     /locus_tag="ACHKUN_000944"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO6659253.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17767..18624
                     /gene="murR"
                     /locus_tag="ACHKUN_000945"
     CDS             17767..18624
                     /gene="murR"
                     /locus_tag="ACHKUN_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO6659254.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18753..19544
                     /gene="ucpA"
                     /locus_tag="ACHKUN_000946"
     CDS             18753..19544
                     /gene="ucpA"
                     /locus_tag="ACHKUN_000946"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO6659255.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19715..20731
                     /gene="cysP"
                     /locus_tag="ACHKUN_000947"
     CDS             19715..20731
                     /gene="cysP"
                     /locus_tag="ACHKUN_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO6659256.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20731..21564
                     /gene="cysT"
                     /locus_tag="ACHKUN_000948"
     CDS             20731..21564
                     /gene="cysT"
                     /locus_tag="ACHKUN_000948"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO6659257.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21564..22439
                     /gene="cysW"
                     /locus_tag="ACHKUN_000949"
     CDS             21564..22439
                     /gene="cysW"
                     /locus_tag="ACHKUN_000949"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO6659258.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            22429..23526
                     /gene="cysA"
                     /locus_tag="ACHKUN_000950"
     CDS             22429..23526
                     /gene="cysA"
                     /locus_tag="ACHKUN_000950"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO6659259.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23660..24571
                     /gene="cysM"
                     /locus_tag="ACHKUN_000951"
     CDS             23660..24571
                     /gene="cysM"
                     /locus_tag="ACHKUN_000951"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO6659260.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24574..24942)
                     /gene="yfeK"
                     /locus_tag="ACHKUN_000952"
     CDS             complement(24574..24942)
                     /gene="yfeK"
                     /locus_tag="ACHKUN_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO6659261.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25047..25898
                     /gene="pdxK"
                     /locus_tag="ACHKUN_000953"
                     /gene_synonym="thiJ"
     CDS             25047..25898
                     /gene="pdxK"
                     /locus_tag="ACHKUN_000953"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO6659262.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25940..26449)
                     /gene="crr"
                     /locus_tag="ACHKUN_000954"
     CDS             complement(25940..26449)
                     /gene="crr"
                     /locus_tag="ACHKUN_000954"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO6659263.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26490..28217)
                     /gene="ptsI"
                     /locus_tag="ACHKUN_000955"
     CDS             complement(26490..28217)
                     /gene="ptsI"
                     /locus_tag="ACHKUN_000955"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO6659264.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28262..28519)
                     /gene="ptsH"
                     /locus_tag="ACHKUN_000956"
     CDS             complement(28262..28519)
                     /gene="ptsH"
                     /locus_tag="ACHKUN_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO6659265.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28903..29874)
                     /gene="cysK"
                     /locus_tag="ACHKUN_000957"
     CDS             complement(28903..29874)
                     /gene="cysK"
                     /locus_tag="ACHKUN_000957"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO6659266.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30059..30820)
                     /gene="cysZ"
                     /locus_tag="ACHKUN_000958"
     CDS             complement(30059..30820)
                     /gene="cysZ"
                     /locus_tag="ACHKUN_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO6659267.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31050..32036
                     /gene="zipA"
                     /locus_tag="ACHKUN_000959"
     CDS             31050..32036
                     /gene="zipA"
                     /locus_tag="ACHKUN_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO6659268.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32107..34122
                     /gene="ligA"
                     /locus_tag="ACHKUN_000960"
     CDS             32107..34122
                     /gene="ligA"
                     /locus_tag="ACHKUN_000960"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO6659269.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34124..34342
                     /gene="ypeB"
                     /locus_tag="ACHKUN_000961"
     CDS             34124..34342
                     /gene="ypeB"
                     /locus_tag="ACHKUN_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO6659270.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34339..35337)
                     /gene="yfeH"
                     /locus_tag="ACHKUN_000962"
     CDS             complement(34339..35337)
                     /gene="yfeH"
                     /locus_tag="ACHKUN_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO6659271.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            35427..36353
                     /gene="yfeR"
                     /locus_tag="ACHKUN_000963"
     CDS             35427..36353
                     /gene="yfeR"
                     /locus_tag="ACHKUN_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6659272.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36392..37156)
                     /gene="yfeN"
                     /locus_tag="ACHKUN_000964"
     CDS             complement(36392..37156)
                     /gene="yfeN"
                     /locus_tag="ACHKUN_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO6659273.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            37405..38238
                     /gene="xapA"
                     /locus_tag="ACHKUN_000965"
     CDS             37405..38238
                     /gene="xapA"
                     /locus_tag="ACHKUN_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO6659274.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            38298..>39542
                     /gene="xapB"
                     /locus_tag="ACHKUN_000966"
     CDS             38298..>39542
                     /gene="xapB"
                     /locus_tag="ACHKUN_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO6659275.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRA"
BASE COUNT         9213 a        10000 c        10968 g         9361 t
ORIGIN      
        1 catgaccatc accacgccaa ccggtgaagg ggtaaccagc gcgcggacgt ttgtccgtct
       61 gcgtcgctgt gtattagtcg atgcgtttcg cattgtttaa ggagtaaccg atggcgcacg
      121 acgaacaatg gctcacccca cgtctgcaaa cggcggcaac gctgtgtaac cagacgcctg
      181 ccgcgacgga atctccgctg tggctggggg tggatctggg cacctgcgat gtggtgtcga
      241 tggttgttga ccgcgacggt cagccggtgg cggtatgcct cgactgggcc gatgtggtcc
      301 gcgacggcat cgtctgggat ttcttcggtg cggtcaccat tgttcgtcgc catctcgaca
      361 ctcttgagca gcaattcggt cttcgtttta gccatgcggc gacttcattt cctccaggta
      421 cggacccgcg tatttccatc aacgtgctgg aatctgccgg gctggaagtg agccatgtgc
      481 tggatgagcc aaccgcagtc gcggatctgc tgcaactgga caacgccggt gtagtggata
      541 tcggcggcgg cactaccggc attgccatcg tgaaaaaggg caaggtgacg tactcggcgg
      601 atgaagccac cggcggacat cacatctctc tgactctcgc cggaaatcgt cgtatttcgc
      661 tggaagaggc agagcagtac aagcgcggtc atggtgacga gatttggccc gcggtgaaac
      721 cggtgtacga aaaaatggcg gacattgtcg cccggcatat tgaagggcag ggcattactg
      781 atttatggct ggcgggcggc tcttgtatgc aaccgggcgt ggcggaactg tttcgcaaac
      841 aattcccggc gttacaggtg catttaccgc agcacagttt gtttatgacc ccgctggcga
      901 tcgccagtag tgggcgagag aaagcggagg ggctatatgc aaaatgaatt gcagaccgcg
      961 ctctttcagg cgttcgatac cctgaatctg caacgggtaa aaacatttag cgttccaccg
     1021 gtgacgcttt gcggtccggg ctcggtgagc agttgcggac agcaagcgca aacgcgtggg
     1081 ctgaaacatc tgttcgtgat ggcagacagc tttttgcatc aggcagggat gaccgccggg
     1141 ctgacgcgta gcctggccgt taaaggtatc gccatgacgc tctggccatg tccggtgggc
     1201 gaaccgtgca tcaccgacgt gtgtgcagcc gtggcgcagt tgcgtgagtc aggctgtgat
     1261 ggggtgatcg catttggcgg cggctcggtg ctggatgcgg cgaaagccgt ggcgttgctg
     1321 gtgacgaacc cggatagcac gctggcagag atgtcagaaa ccagcgttct gcaatcgcgc
     1381 ctgccgttga ttgccattcc cactaccgcc ggaaccggct ctgaaaccac caacgtaacg
     1441 gtgattatcg atgcggtgag cgggcgtaag caggtgttag cccatgcctc gctgatgccg
     1501 gatgtggcga ttctcgacgc cgcattgacc gaaggtgtgc cgtcgcatgt cacggcgatg
     1561 accggcattg atgcgttaac ccatgccatt gaagcataca gcgccctgaa cgctacaccg
     1621 tttaccgaca gtctggcgat tggtgccatt gcgatgattg gcaaatcgct gccgaaagcg
     1681 gtgggctacg gtcatgacct tgccgcgcgc gagagcatgt tactggcttc atgtatggcg
     1741 ggaatggcgt tttccagtgc ggggcttggg ttgtgccacg cgatggcgca tcagccaggg
     1801 gcggcgctgc atattccaca cggtctggcg aacgccatgt tgctgccaac ggtgatggaa
     1861 tttaaccgga tggtttgtcg cgaacggttt agtcagattg gtcgggcact gcgaactaaa
     1921 aaatccgacg atcgtgacgc tattaacgcg gtaagtgagc tgattgcgga agttgggatt
     1981 ggtaaacgac tgggcgatgt tggcgcgaca tctgcgcatt acggtgcatg ggcgcaggcc
     2041 gcgcaggaag atatttgtct gcgcagcaac ccgcgtaccg ccagcctgga gcagattgtc
     2101 ggcctgtacg cagcggcgca ataaatgccg gatgcgacgc ttgccgcgtc tgatccggcc
     2161 ttcgggattg cacatgtagg gcggataagg cgtttacgcc gcatccgcca ataaataaca
     2221 actaacaggg agtaaaggcg atgggaatta acgaaatcat catgtacatc atgatgttct
     2281 ttatgctgat agctgccgta gacaggatcc tgtcgcagtt cggcggttct gctcgtttcc
     2341 tcggtaagtt cggtaaaagt atcgaaggat caggcggtca gttcgaagaa ggctttatgg
     2401 caatgggcgc actgggcctg gcgatggtcg gtatgaccgc gctggcaccg gtactggctc
     2461 acgtactcgg gccggtaatt attccggttt acgaaatgct cggcgctaac ccatcgatgt
     2521 tcgccggaac actgctggcg tgcgatatgg gcggcttctt cctcgccaaa gagctggcgg
     2581 gcggcgacgt agcagcgtgg ctatactctg ggttaattct cgggtcgatg atggggccaa
     2641 cgattgtgtt ttccattccg gtggcgctcg gcattatcga accttctgac cgtcgttatc
     2701 tggcgctcgg cgtgctggcg ggcattgtga ccattccgat tggctgtatt gctggtggtc
     2761 tggttgctat gtactccggt gtgcagatca acggtcagcc ggtggaattc actttcgccc
     2821 tgatcctgat gaacatgatc ccggtgatca ttgtcgcgat tctggtggcg ctggggctga
     2881 aattcatccc ggaaaaaatg atcaacggct tccagatctt cgccaaattc ctcgtggcat
     2941 tgatcaccct cggtcttgcc gctgcggtag tgaaattcct ccttggctgg gaactgatcc
     3001 ccggactgga tcctatcttt atggcccctg gcgataaacc cggtgaagtg atgcgcgcca
     3061 ttgaagttat cggttctatc tcctgcgtgc tgttaggggc gtatccaatg gtgctgctgc
     3121 tgactcgctg gtttgaaaaa ccgctgatga gcgtcggtaa agtgctgaat atgaacaaca
     3181 tcgcggcagc cggcatggtg gcaacgcttg ccaacaacat cccgatgttc ggcatgatga
     3241 agcagatgga tacccgcggc aaagtcatca actgcgcctt cgccgtttcc gctgctttcg
     3301 ccctgggcga ccacttaggc ttcgccgctg ccaacatgaa cgccatgatc ttcccgatga
     3361 ttgtcggcaa gttgatcggc ggcgtaacgg cgattggcgt ggcgatgatg ctggtgccaa
     3421 aagaagacgc gaccgcgact aaaaccgaag cggaggcaca atcgtgaaca ctcgccagct
     3481 attgagcgtc ggtatcgata tcggcaccac caccacccag gtgattttct cccacctgga
     3541 gctggttaac cgtgcggcgg tgtcgcaggt gccgcgctac gaattcatta aacgcgaaat
     3601 tagctggcaa agtccggtgt tctttacccc tgtcgataaa cagggcggtt taaaagaagc
     3661 ggaactgaaa accttaatac tcgagcaata tcaggctgcg ggtattgcgc cggaaagcgt
     3721 tgattctggt gccatcatca tcaccggtga aagcgcgaaa acccgcaatg ctcgcccggc
     3781 ggtgatggcg ctctctcaat cgctggggga ttttgtcgtt gccagcgccg ggccgcacct
     3841 cgaatccgtg atcgccggtc acggagctgg ggcgcaaacc ctttctgaac aacggctgtg
     3901 tcgggtactg aatatcgaca tcggcggtgg caccgcgaac tacgccctgt tcgatgccgg
     3961 aaaaatcagc ggcactgcct gtctcaacgt cggtggtcgc ctgatggaaa ccgacagcca
     4021 ggggcgcgtg gtttacgctc ataaaccggg gcagatgatt gtggatgagt gcttcggtgc
     4081 aggcactgac gcccgttcgc tgaccggcgc gcagctggtg caggttaccc ggcggatggc
     4141 ggcgctgatt gtcgaagtga ttgacggaac gctttcgccg ctcgcgcagg cattgatgca
     4201 aaccggtttg ctgcccgcag gtgttacgcc cgaaatcatt acgctttctg gaggcgtggg
     4261 cgaatgttat cgccaccagc ccgccgaccc gttctgtttt gccgatattg gcccacttct
     4321 ggcaacggcg ctgcatgacc atccgcgcct gcgtgagatg aatgtgcagt ttccggcgca
     4381 aaccgtacgc gccacggtga ttggcgcggg tgcacatacc ctttcgctct ctggcagcac
     4441 aatctggctg gagggcgtac aactgccgct gcgcaatttg ccggtggcga tcccgattga
     4501 tgaaacggat ctggtgagtg cctggcaaca ggcgctgctt cagctggatc ttgatcccaa
     4561 aactgacgcg tacgtgctgg cgcttcccgc ctcgctgcct gtgcgttacg ccgcggtact
     4621 gacggtcatc aacgcgctgg tcgatttcgt cgcgcgtttt ccgaatccgc atcccctgct
     4681 ggtggtggcc gggcaggact ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca
     4741 acaactcccg ttggcagtca ttgacgaagt gattgtccgc gcgggggact atatcgacat
     4801 tggtacgcct ctttttggcg gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc
     4861 ttcctgaggg aacgacttat gaaactaaag accacattgt tcggcaatgt atatcagttt
     4921 aaggatgtaa aagaggtgct ggctaaagcc aacgaactgc gttcggggga tgtgctggcg
     4981 ggcgttgcag cggcaagctc acaggagcgc gtggcggcaa agcaggtgtt gtcggaaatg
     5041 accgtagcgg acatccgcaa taatccggtg attgcctatg aagatgactg cgtgacgcgg
     5101 ctgattcagg acgatgttaa cgaaacggcc tacaaccaga ttaaaaactg gagcatcagc
     5161 gaactgcgtg agtatgtgct gagcgatgaa accagcgtgg acgacattgc ctttacccgc
     5221 aaagggctga cctcggaagt ggtcgcggcg gtagcgaaga tttgctccaa cgcggacctg
     5281 atctacggcg cgaagaaaat gccggtaatc aaaaaggcca ataccaccat tggtattccg
     5341 ggcaccttca gcgcccgttt gcagccgaac gatacccgtg acgacgtgca aagtatcgct
     5401 gcgcaaatct atgaagggct ttcctttggg gtgggcgatg cggtgatcgg cgttaacccg
     5461 gtaactgacg acgtggaaaa cttaagccgc gtgttggaca ccatttacgg cgtgatcgac
     5521 aaattcaaca tcccaactca gggctgcgta ctggcgcacg tcaccaccca gatcgaagcg
     5581 attcgtcgcg gcgcgcctgg cggactgatt ttccagagta tctgtggcag cgaaaaaggg
     5641 ctgaaagagt ttggcgtgga actggcgatg ctcgacgaag cgcgcgcagt gggtgcagag
     5701 ttcaaccgta tcgccgggga aaactgcctc tacttcgaaa ccggacaagg ctctgcgcta
     5761 tccgctggcg ctaacttcgg cgctgaccag gtgacgatgg aagcacgtaa ctatgggctg
     5821 gcgcgtcatt acgatccgtt tatcgtcaac accgtggtcg gctttattgg gccggagtat
     5881 ctctacaacg accgccagat tatccgtgct ggcttagaag atcactttat gggcaagctg
     5941 agcggcatct ctatgggctg tgactgctgt tacaccaacc acgctgacgc tgaccagaac
     6001 ctcaacgaaa acctgatgat cctgctcgcc accgcaggct gcaactacat catggggatg
     6061 ccgctgggtg atgacatcat gctcaactac cagaccaccg cattccacga taccgccact
     6121 gtgcgtcagt tactcaacct gcgtccgtca ccggagtttg aacgctggct ggaaagcatg
     6181 ggcattatgg caaacggtcg cctgaccaaa cgggcgggcg atccgtcact gttcttctga
     6241 tgacgcggag ataacacatc atggatcaaa aacagattga agaaattgta cgcagcgtga
     6301 tggcgtcaat gggacaaacg gcccccgcgc cgtcagaagc aaagtgcgcc accaccaact
     6361 gtgcggcacc ggtgacctcg gaaagctgcg cgctggattt aggttctgcg gaagcaaaag
     6421 cgtggattgg tgttgaaaac ccgcatcgcg cagacgtatt aacagaactg cgtcgcagca
     6481 ccgtggcccg cgtttgtacc ggtcgtgccg gtccgcgtcc gcgtacgcag gcgttgctgc
     6541 gtttcctggc cgatcactct cgttcgaaag ataccgtact gaaagaagtg ccggaagagt
     6601 gggtgaaagc gcagggcttg ctggaagtac gctccgaaat tagcgacaaa aatctctacc
     6661 tgactcgccc ggatatgggc cgccgcctgt gtgcagaagc tgttgaagcg ctgaaagcac
     6721 agtgtgttgc taatccggac gtacaggtcg ttatttctga tggcctgtca actgatgcca
     6781 tcaccgtgaa ctacgaagag atcctgccgc cgctgatggc gggcctgaaa caggccgggc
     6841 tgaaagtcgg cacgccattc tttgttcgtt atggtcgcgt gaagattgaa gatcagatcg
     6901 gtgagatcct cggcgcgaaa gtggtgatcc tgctggtggg ggaacgtccg gggttagggc
     6961 agtcagaaag cctctcctgc tacgccgttt actcgccgcg tatggcgacc accgttgagg
     7021 ccgatcgcac ctgtatctct aacattcacc agggcggcac gccgccggtt gaagccgcag
     7081 ccgtcattgt ggatttggcg aaacgtatgc tggagcagaa agcatccggc atcaacatga
     7141 cccgataagg aggcatcatg ccagctttag atttgattcg accgtcggta accgccatgc
     7201 gggtgattgc ctccgttaac gctgaatttg cgcgtgaact aaaattgccg ccgcatattc
     7261 gtagcctcgg gctgatttct gccgattctg acgacgtcac ctacattgcg gctgacgaag
     7321 cgaccaaaca agcgatggtc gaagtggtat atggtcgctc gctgtatgcg ggagcggcac
     7381 acggcccgtc accgactgcc ggtgaagtgc tgattatgct tggtgggcca aacccggcgg
     7441 aagtgcgtgc cggtctggat gcgatggttg cacatattga aaatggcgca gctttccagt
     7501 gggccaatga cgcagaaaac accgccttcc tggcacatgt agtttcgcgt accggttctt
     7561 atctctcatc aaccgccggg atcacgcttg gcgatccgat ggcgtatctg gtggcaccgc
     7621 cgctggaagc gacctacggc attgatgcag cgttgaaatc tgccgacgta cagctggtga
     7681 cctatgtccc gccaccgtct gaaaccaact actcggcagc atttttaacc ggtagccagg
     7741 ccgcgtgtaa agcagcctgt aacgccttta ccgatgcagt gctggaaatc gcgcgtaatc
     7801 caatccagcg tgcgtaacgg aggttgccga tgatcaatgc actgggattg ctggaagtgg
     7861 acggcatggt cgccgcgata gatgcagcgg atgccatgct caaagcagct aacgttcgtc
     7921 tgctcagtca cgaagtgctt gaccctggtc gcttaacgct ggtggtggaa ggcgatctgg
     7981 cggcgtgtcg tgcggcgctg gacgcaggtt gtgctgccgc gatgcgtacc gggcgtgtca
     8041 tcagccgcaa ggagatcggt cggccagacg atgacaccca gtggctggtt actggcttta
     8101 accgccagcc gaagcaaccc gtaagggaac ccgacgcgcc agttatcgtc gcggaatctg
     8161 ctgacgagtt gttggcgctg ttaacatcag tacgtcaggg aatgacggca ggagaagtgg
     8221 ctgcccactt tggctggccg ctggaaaaag ccagaaatgc gctcgaacag ctcttttctg
     8281 ccgggacgtt acgtaaacgc agtagtcgtt atcgtctcaa gccccattaa cctgtcggag
     8341 gtgccgggtg tcctacaaca cccggcatta acatcatgaa aaagacccgt acagccaatt
     8401 tgcaccatct ttatcatgaa cccttacccg aaaacctgaa gctcacgccg aaggtcgaag
     8461 tggataatgt tcatcaacga cagacaacgg atgtctatga acatgcttta acgattaccg
     8521 cctggcagca gatttacgat cagctgcatc cgggcaagtt tcattgtgaa tttacggaaa
     8581 ttctactcga tgatattcag gtttttcgtg aatacaccgg tctggcgctg cgtcagtcgt
     8641 gcctggtctg gccgaactcg ttctggtttg gcattccggc gacgcgcggt gagcagggat
     8701 ttatcggttc gcaatgtctg ggaagcgcgg aaatcgccac ccgccctggt ggcactgaat
     8761 ttgaactgag cacgccggat gattacacga tcctgggcgt ggtgctttct gaagatgtca
     8821 tcacccggca ggctaacttt ttgcataacc cggatcgggt attacatatg ctgcgtagcc
     8881 agtcggcgct ggaagtgaaa gagcagcata aagccgcgct gtggggcttt gtccaacagg
     8941 cgctggcgac gttttgcgag aacccggaaa atctccatca gcctgcagtg cgaaaagtgc
     9001 tgggggataa tttgctaatg gcgatggggg cgatgctgga agacgcgcaa ccaatggtga
     9061 cggcggaaag catcagtcat cagagttacc gtagattact ttcccgcgcc cgtgaatatg
     9121 tgctggaaaa tatgtctgag ccggtgacgg tgctggactt gtgtaatcaa ctgcatgtca
     9181 gtcgccgcac gctacaaaac gcgtttcacg ctattttagg cattggccca aacgcgtggc
     9241 tgaaacgcat tcgcctgaac gccgtacgcc gcgaactgat aagcccgtgg tcgcaaagca
     9301 caacggtaaa agacgccgcc atgcagtggg gattctggca tctggggcaa tttgccacgg
     9361 attaccagca gctgtttgcc gagaagccgt cgttgacgtt gcatcagcgg atgcgggaat
     9421 gggggtgagg gagttacacc caatccctga ccttaataaa ctcacttaac gccgcttctg
     9481 ggctgccatc ttttggctga taatcatact cccagcgtac cagtggcggc attgacatca
     9541 ggatcgactc ggtgcgcccg ccagtttgca ggccaaacag agtgccgcga tcccagacca
     9601 gattgaactc gacataacga ccgcgacggt agagctgaaa attgcactcg cgctcgccgt
     9661 aggccatcgc tttacgtcgc tctacaattg gtaaataagc gtcggtgtag cctttgccta
     9721 ccgcctgcat aaaggcaaaa cagtggtcga aatctggcgt gttcagatca tcaaagaaca
     9781 gcccgccaat accgcgctgt tcgttgcgat gtttgaggta gaagtaatcg tcgcaccact
     9841 ttttgtaacg gggataaacg tcttcaccaa atggcaggca caggtcacgg gcggtgcggt
     9901 gccagtgaat ggcgtcttct tcaaaaccat agaaaggggt taaatcgaag ccgccgccaa
     9961 accaccagac gggctctgca cccggttttt cggcaataaa aaaacgcaca ttcgcgtggc
    10021 tggtgggaac atacgggtta tgcggatgca ctaccagtga aacgcccatc gcctcgaaac
    10081 tgcgcccggc aagttccggg cgatgagcgg tggcggaagc tggcatcgcc tcgccgtgga
    10141 cgtgagaaaa gttgacgcct gcctgttcga aaacaccacc attacgcaac acccgactac
    10201 gcccgccgcc gccagcttcg cgctgccaac tatcttcgac aaattctgcg ccatcgacgg
    10261 cggtcagctg ctgacaaatc gtatcctgaa ggttgagcag aaactgttta acctggtgtg
    10321 cgtcgggttt cataacttat cgctttttcg aatgtgcttt ctggttgtcg aaccagtgga
    10381 aataactgat cacgccttca gcaatcgctg tggcgatttt ctgacgaaac gccgccgtgc
    10441 ctaacagccg ctcttcttcc gggttggtga taaacgaggt ttccaccagc accgaaggaa
    10501 ccgacggcga tttcaacacc acaaacgcgg cttgttcggt gttgcggctg tgcagtttat
    10561 gcaccggctt aatctttttc agaatatgcg agccgagcgt caggctgttt ttaatagtat
    10621 cggtttgcac cagatcaaac agcacttgct gcaataggtg atccttgtca gtcgcctttt
    10681 taccggcaac ttcatcggcg cggttttcgc gttcagacag gtatttcgcc attgcgctac
    10741 ttgccccacg gttagagagg gcaaataccg aagcaccggc agctttcggg ttggtaaagc
    10801 catcggcatg aattgacata aacagatctg cgccatgttt atgggcgatt tcaacgcgat
    10861 cgtaaagtgg gataaacgta tcgccagaac gcgttaaacg cgcatcaatc ccatgattac
    10921 gcaaaatgga acggacgttt ttagcaatcg ccagcaccac atgtttttct ttcgaaccgt
    10981 tgcgtccgat cgctccggta tcaattccgc cgtggcctgg atcgagaaca acgacacgtt
    11041 tgccgccaga ttttttggct ttcggcttgc tgtgtccgtt gctggttttt aaaggttcgt
    11101 ctttggcgat ggcttgcgac attcctgaca acgtcagggc agccaaaccg gctttcagca
    11161 cctggcggcg cgaagtgagt gtttttagtg gtttaaaagt gctcatacgg cctgagttgt
    11221 aataataaag ttccagatgt tatatcgtat cgcgtaatcc gttacgacgg tttgatgttg
    11281 agaattgttt tacttttcat ttcaatagtt gacaactccc cattatgcca ctttttcaac
    11341 cgattttcgt cagatattgg ctaattcgac cgttcacgcc ataatcagcc ttttaacccg
    11401 caaaaaggta acgaatacca tggagatacg cgtatttcgc caggaagatt tcgaagaggt
    11461 catcaccctt tgggagcgtt gcgacttgct gcgtccgtgg aacgatccgg aaatggacat
    11521 cgagcgtaag atgaaccatg acgtcagttt gtttttggtc gctgaggtaa acggtgaagt
    11581 ggtcggaacg gtgatgggcg gttatgacgg gcatcgcggg tctgcttatt atcttggcgt
    11641 gcatccagag tttcgtgggc gtgggattgc caatgcgttg cttaatcggc tggagaaaaa
    11701 gctgattgct cgtggctgcc cgaaaattca gatcaatgtg ccggaagata acgacatggt
    11761 gctcggaatg tatgaacgcc tgggatatga acacgccgac gtgctgagtc tgggtaagcg
    11821 tttgattgaa gatgaagagt actgagttcc atcctgtcca ttatgatgcg cacggacgcc
    11881 tgcgtttacc cttgctcttc tggcttgtgc tattgcttca ggcgcgaacc tgggtgctgt
    11941 ttgtcattgc cggtgcgtcg cgcgagcagg gtactgcgct gctaaatctg ttttatcccg
    12001 atcacgataa tttctggctg gggctgattc caggcattcc ggcggtgctg gcgtttttgt
    12061 tgagcggtcg gcgggctaca tttcctcgca cttggcatgt gctctatttt ctgttgctgt
    12121 tggcgcaggt ggttttactt tgctggcaac cgtggctgtg gctgaacggt gaatccgtta
    12181 gcggtatcgg tctggcgctg gtagtggcgg atattgtggc gttaatctgg ctgctgacca
    12241 atcgacgttt acgcgcttgt tttaatgaag aaaaagaata acggcacttt ttggtgaatt
    12301 tgcactccaa gcaacgttat tgaataacca aaggaagtga catgaaatcg ctgcgtttaa
    12361 tgttatgcgc tatgccgttg atgctgaccg gctgttccac gatgtcgtca gttaactggt
    12421 cggccgctaa cccgtggaac tggtttgggt cgtccaccaa agtgagcgag cagggcgttg
    12481 gtgaattaac ggcgtccaca ccactgcaag aacaagccat tgccgatgcg cttgatggcg
    12541 attatcgcct gcgcagcgga atgaaaaccg cgaacggcaa cgtggtgcgc ttttttgaag
    12601 tgatgaaggg cgacaacgtg gcgatggtga ttaacggcga tcaaggcacg atcagtcgca
    12661 ttgacgtgct ggatagcgat attcctgctg acaccggtgt taaaatcggt acaccgttta
    12721 gcgaccttta cagcaaagca tttggcaatt gccaaaaagc tgatggtgat gataatcgtg
    12781 ccgtcgaatg taaagccgaa ggcagtcaac atattagcta ccagttcagc ggggaatgga
    12841 gtggtcctga agggttaatg ccttcggacg ataccctgaa aaactggaaa gtcagtaaaa
    12901 ttatctggcg gcgttaattt gcgtctgaac aaaccgccgc cgcgaaaaaa cacgtaaaat
    12961 agcgctcaac aatgccacgg attgcgtggc attattattt tcaggaggaa caatgtctca
    13021 ggttcagagt ggcattttgc cagaacattg ccgcgcggcg atttggatcg aagccaacgt
    13081 gaaaggggaa gttgacgccc tgcgtgcggc cagtaaaaca tttgccgaca aactggcaac
    13141 ttttgaagcg aaattcccgg acgcgcatct tggtgcggtg gttgcctttg gtaacaacac
    13201 ctggcgcgct ctgagcggcg gcgttggggc agaagagctg aaagattttc cgggctacgg
    13261 taaaggcctt gcgccgacca cccagttcga tgtgttgatc cacattcttt ctctgcgtca
    13321 cgacgtaaac ttctctgtcg cccaggcggc gatggaagcc tttggtgact gcattgaagt
    13381 gaaagaagag atccacggct tccgttgggt tgaagagcgt gacctgagcg gctttgttga
    13441 cggtacggaa aacccggcgg gtgaagagac gcgtcgcgaa gtggcggtta tcaaagacgg
    13501 cgtggatgcg ggcggcagct atgtgtttgt ccagcgttgg gaacacaacc tgaagcagct
    13561 caaccggatg agcgttcacg atcaggagat gatgatcggg cgcaccaaag aggccaacga
    13621 agagatcgac ggcgacgaac gtccggaaac ctctcacctc acccgcgttg atctgaaaga
    13681 agatggcaaa gggctgaaga ttgttcgcca gagcctgccg tacggcactg ccagtggcac
    13741 tcacggtctg tacttctgcg cctactgcgc gcgtctgcat aacattgagc agcaactgct
    13801 gagcatgttt ggcgataccg atggtaagcg tgatgcgatg ttgcgtttca ccaaaccggt
    13861 aaccggcggc tattatttcg caccgtcgct ggacaagttg atggcgctgt aagcgtttct
    13921 gattcattga ttaaaggcca ggcagtaaca ttactgactg gcctttttat tacttttgct
    13981 ttaacgccgc atacacctga tcaactaccc aaccatacgt tgcaatcggc agctgaccgc
    14041 tttcgaacat attgggattc ttttgcggat cggcaaccgg cgaatgtggc ttgttgctta
    14101 acatcacaat cgccatatga ttcaccggat cgataacggt caccgttccg gtccagccag
    14161 tgtgaccgta ggtttgcggg cttgccagcg tgccaaacgt cggcgtcatg gtggcattac
    14221 cattcacgcg ccagccgagg ccaaaagtgg catcttcctt agagctggtg gtgaacatct
    14281 tcaccgtttc cgcactgaac agctgcacat cgccatagcc cccgccgttc agcatcgttt
    14341 gcattaacac cgcaatatcg ccggtattgg aaaacaaacc tgcgtgcccg gaaacgccgc
    14401 ccatcgaata aaaggctttt tcatcgtgca cctgacccca gagagtggag gtgcggatat
    14461 tcgggaaatg gatcacgcca tcgcgggtat tgccgttcaa ttccgtggcg gcaatttgct
    14521 gcggtttaaa gcctttcagc agcgggttaa ataccgtatg tgtcaggccg agcgggcgat
    14581 aaatcgactc ttcaacatag cggtcaagcg gctgaccggt gaccgactcg acgataaatc
    14641 caagcagcat ataatcgaca tcactgtaaa tatgcttgct gccgggctga tactccagcg
    14701 gcgtgcgctt gatcatctcc agcgtctggc ctttatcctg ggaatataac gcgcccgcga
    14761 ccgctttatt cggatattgt gggtccgccg ggaaaccgcc gctgtgatgc agcaggtcag
    14821 aaatccgcag cgtgtttttg cctttgatgg tgtcatttgg actatcggca aaccccggaa
    14881 tatatttcgc aatccgatcg tcaggatgca gtttgccttc gctcatcagc ttttgcaggg
    14941 cgaagttcgt ggcgtacatt ttggtgtttg aggccaggtc atacagcgtc ccggtggtgg
    15001 ctttgacagg ctgttccatc aacacgctgc catcgtactt tttcgccgct ccccaggctt
    15061 tgcgatacac aatctgatta tctttaatga tcagcaggtt tacgccggga taaccggcgt
    15121 ccacttgctg gctaatccag cgatccatct ggttaagccg ttcgacgttg aaccctgctt
    15181 tctctggcga actttctgtc agaacagggt attttgcggc gctgacgcta caactaacag
    15241 ccagcagaga aagatagagc attgtccgtt tcatgatatc agtccagatt gacgttacgg
    15301 cagccaaaga gcgtggtgaa aataaacccg caaacccatg ccaccagaat cccgccagca
    15361 taaaccgcca ttgccggaag gatgccttgt gcggaagtca tcagcggcag cgccaccaga
    15421 ccagacggcc caaaggcgct gtttaagccc atcggcagac cccaccaggc tatcaggccg
    15481 ataaacaaac cgcccgctgc gccgcctaaa caggcggtaa taaacggttt catgcggggc
    15541 agggtgacgc cgtaaatcaa cggttcacca acgcccagca gaccgggaat aatcgccccg
    15601 cgtacctgac tgcgtaatgc actgtgcggt tgcgcccgcc agtagagtgc cagcgccgcg
    15661 cccacctggc ccgcgcctgc cattgaaagg atgggaaata agctgttgaa tccctggctg
    15721 tccattaacg cgaggtaaac aggaataaag ccctgatgca cgccaaatac cacggcgatc
    15781 aggaacagcc cggctaaaac cgcacaaccg aacggattac tgttcaggtg cataaacagc
    15841 catgacatac cttcgaataa ccagccaccc agcggcataa tgatcaggta cgcaagcgta
    15901 gcggtgatca gcagggtgat taacgaggtc agcagcatgt cgagatcgtc cggcataaag
    15961 cggcgcacca tgccttcaat gcgggcgcag gcccacgcgg caatcagcac gccgataata
    16021 ttgccgcgcg gatcgatggg cagaccaaag aaatcgtgaa aaccggcgta gtaaccggtg
    16081 gttgcagcgg ggttataacc gagcaaaaag agtgcggcaa taattgcgcc attgacgccc
    16141 gtgccgccaa atgcctgggc ggcgttatag cccaccagaa tcaccaggaa agtgaacaaa
    16201 cctttgctga acaccttcat aaaattcagc gcgtcgggta gcgtgccctg agcatctgcc
    16261 ggaacgtgca tcaccgtggc aattaacgtc gctatcccca gcagcagacc ggcggcaata
    16321 aaaccgggga tcagcggcgt gaagatggtg gcgaatttgg cgagaaattg ttgcacgccg
    16381 gaggtttgtt tggcttttaa ctgacgtttg ttctgggcgg cgatttctgc ggcatcctgt
    16441 accggtgcct cgcccagcag ctcgctcatc gcttttgcgg cacgatgcgc tttaccaggc
    16501 ccaaaaacga cctgcacctg gtcgctggtc aaaatgacgc ccttcacgcc ttccagcgtt
    16561 ttgatattgg gatcaaccag tgaactgtca tgtacaccca gacgcaggcg cgtcatacag
    16621 ttaccacaac tggcgatatt tcccggtccg ccgacacggg taagaatggt gttcagaagt
    16681 tcactgctga tctctttggc catgggttat tccttgtcta aaacctgacg aataaagccg
    16741 ccgtgttgat ccaggcgttt tttagcttct gcggcatcga gatttttcag caccatcaca
    16801 atggccgttt tacagttgcg ctcgcaggca attaacgccg cttccgcttg ttcggcatta
    16861 catccggtgg cgtttttaac gatattgacc tggcgcacat gcagtttttc gttggtggcg
    16921 accacatcga ccatcaggtt gccgaacact ttgccagatt taatcatcag cccggtggaa
    16981 agcatattga gcaccagttt ctgcgctgtg cctgctttca ttcgtgaaga accagtgacc
    17041 acttcggcac ctacaatcgg tgtaatggca aactcggcgg tggtggaaac ggcactcccc
    17101 ggattacagg aaattcccac tgtgcggcag ccgagctggc gtgcgtattc cagtccggca
    17161 atcacatacg gcgtgcgacc gctggcagca atgccaacca ccacatcctg tgccgttaaa
    17221 ttaatatttt tcagatcgtt aacaccacct tcccggctat cttcagcgcc ttccaccgcg
    17281 tgctgaatgg catattcgcc tccagcaatc aaaccaacca ccagacccgg tttcacgccg
    17341 taggtgggcg gacattcgct ggcatccaga atccccagac gaccggatgt tcccgcaccg
    17401 agataaatca gacgaccgcc gccgctgacc tgggcgtgga taacatcgat cgccgcggcg
    17461 atatccggca ggacgcgctc aacggcaagc ggtacggttt tatcttcatc gttgataatc
    17521 cggcacattt ccagcgtcga tacgcggtca atttcagccg aggcggtgtt cgagccttca
    17581 gtaatcatct tttctagttg catcggtggt gaccttacta tttacgatca aataattgca
    17641 gatgtatgga atcatatatc ccgaattgac agacttaaag gaatcattga gtctacactc
    17701 gcagagtgtg aagagcgtca taagaactta agcggaattt atgctgccag cccaggagag
    17761 tggaagatgt tgtacctgac gaaaataagc aatgcgggga gtgaattcac cgaaaacgag
    17821 caaaaaatcg ctgactttct gcaggccaac gtgagtgagc tgcaatccgt ctcttcgcgc
    17881 aagatggcga aacagctcgg catcagccag tcgagtattg tgaagtttgc ccagaagctc
    17941 ggcgcacaag gatttactga attgcgtatg gcgttaattg gcgaatacag tgccagccgg
    18001 gagaaaacga atgccacggc gctgcacctg cacagttcga ttaccagtga tgattcgctg
    18061 gaagtgattg cccgcaaact gaatcgcgaa aaagagctgg cgctggagca aacctgcgcg
    18121 ttgttcgact acgcgcggct gcaaaaaatc attgatgtca tcagtaaagc gcaatttatt
    18181 cagataacgg gcctgggcgg atcggcgctg gtggggcgcg atctgtcgtt caaactgatg
    18241 aaaattggat atcgcgtggc ctgcgaagcc gacacgcacg tacaagccac cgtttcccag
    18301 gcgttgaaaa aaggcgatgt acaaatcgct atctcttaca gcggcagtaa aaaagagatt
    18361 gtcttgtgcg cagaagcggc acgaaagcag ggggcaacgg tgattgccat tacttctctg
    18421 gctgactctc cgctgcggcg gctggcgcat ttcacgctcg ataccgtttc cggtgaaacc
    18481 gaatggcgta gctcgtcgat gtctacccgc acggcgcaaa attctgttac cgatttactg
    18541 tttgttggtc ttgtgcaact caatgatgtc gagtcattga aaatgattca gcgcagcagt
    18601 gagctaacac aacgcctgaa atgatgcata aagcagcgac tggattgaga ttttcctgaa
    18661 ttagtgagct gatccgcagc aatattttgt ttatcctgta ttttcagagg gaatggagtg
    18721 taacgctctg tattaacaag gagagcatta aaatgggtaa actcacgggc aagacagcac
    18781 tgattacggg cgcattgcag ggaattggcg aaggaattgc cagaactttt gcgcgtcatg
    18841 gcgcaaacct aatcttgctg gatatctccc ctgagatcga aaagctggcg gacgaactgt
    18901 gtggtcgtgg tcatcgctgt acggcggttg tcgccgatgt gcgtgacccg gcgtcggtag
    18961 ccgcagctat caaacgcgcg aaggaaaaag aagggcgcat tgatatcctg gtgaataacg
    19021 caggcgtttg tcgtctgggc agtttcctcg atatgagcga tgaagatcgc gatttccata
    19081 ttgacatcaa tattaaaggc gtatggaacg tcacgaaggc ggtgctgcca gagatgattg
    19141 cgcgcaaaga tggtcgcatt gtgatgatgt cttcagtcac tggtgatatg gtggccgatc
    19201 cgggtgaaac ggcgtacgcc ttaacgaaag cggcgattgt tggcctgaca aaatcgctgg
    19261 cggtggagta cgcgcaatcc ggtattcgcg ttaacgccat ctgcccgggg tacgtccgca
    19321 cgccaatggc ggaaagcatt gcccgccagt cgaacccgga agatccagag tcggtgctga
    19381 ctgatatggc aaaagcaatc ccgctgcgtc gcctcgccga tccgctggaa gtcggcgaac
    19441 tggcggcctt cctcgcatcg gatgaatcca gctatttaac cggtacacag aatgtgattg
    19501 atggcggcag cacactgccg gagacggtta gcgtcggtat ctgattcacc tctgtttcct
    19561 ccctgcatgt gtggggagga tttcgtttta aacccggttc ttattccctt ttcaacttcc
    19621 aaatcaccaa acggtatata aaaccgttac tcctttcacg tccgttataa atatgatggc
    19681 tattagaaag tcattaaata tataagggtg cgcaatggcc gttaacttac tgaaaaagaa
    19741 ctcactcgcg ctggtcgctt ctctgctgct ggcaggccat gtacaggcaa cggaactgct
    19801 gaacagttct tatgacgtct cccgcgagct gtttgccgcc ctgaacccgc cgtttgaaca
    19861 gcaatgggcc aaagataacg gcggcgacaa actgacgatt aaacaatctc atgccgggtc
    19921 atcaaaacag gcgctggcga ttttacaggg cttaaaagcc gacgttgtca cttataacca
    19981 ggtgactgac gtacaaatcc tgcatgacaa aggcaagctg atcccggcgg actggcagtc
    20041 gcgcctgccg aataatagct cgccgttcta ctccaccatg ggcttcctgg tgcgtaaggg
    20101 caacccgaag aatatccacg actggaacga cctggtgcgc tccgacgtga agctgatttt
    20161 cccgaacccg aaaacgtcgg gtaacgcgcg ttatacctat ctggcggcat ggggcgcagc
    20221 ggataaagct gacggtggcg ataaagccaa aaccgaacag tttatgaccc agttcctgaa
    20281 aaatgttgaa gtgttcgata ccggcggtcg tggcgcaacc accacttttg ccgagcgtgg
    20341 cctgggcgat gtgctgatta gctttgaatc ggaagtgaac aacatccgta aacagtatga
    20401 agcacagggc tttgaagtgg tgatcccgaa aaccaatatt ctggcggaat tcccggtggc
    20461 gtgggtcgat aaaaacgtgc aggccaacgg tacggaaaaa gcagcaaaag cctacctgaa
    20521 ctggctctac agcccgcagg cgcagaccat catcaccgac tattactacc gcgtaaataa
    20581 cccggaagtc atggacaaac tgaaagataa attcccgcag accgagctgt tccgcgtgga
    20641 agacaaattt ggctcctggc cggaagtgat gaaaacccac ttcaccagcg gcggcgagtt
    20701 agacaagctg ttagcggcgg ggcgtaactg atgtttgcag tttcatcaag acgcgtgctg
    20761 ccgggcttta ccttaagcct cggcaccagt ctgctgtttg tgtgcctgat tttgctgctg
    20821 ccgctctccg cgctggtgat gcagctggct cagatgagct gggcgcagta ctgggaggtg
    20881 atcaccaacc cacaagtggt cgcggcctac aaagtgacgc tgctgtcggc gtttgtggca
    20941 tcgattttta acggcgtttt cggtctgctg atggcgtgga ttttaacacg ttatcgcttt
    21001 ccgggccgca cgctgcttga tgcgctgatg gatttaccct ttgcgctgcc aacggcagtc
    21061 gccggtttaa cgctggcctc gctcttttcc gtaaacggtt tttacggtga atggctggcg
    21121 aagtttgata tcaaagtcac ctatacctgg cttggtattg cggtggcgat ggcctttacc
    21181 agcattccgt ttgtggtgcg taccgtgcag ccggtgctgg aagagttagg cccggaatat
    21241 gaagaagcgg cggaaacgct tggtgcaacg cgctggcaga gtttctgcaa agtggtgctg
    21301 ccggaacttt ctccagcgct ggtggcgggc gtggcgttgt cgtttacccg tagtcttggt
    21361 gagtttggcg cggtgatatt tatcgccggg aacatcgcct ggaagacgga agtgacgtcg
    21421 ctgatgattt ttgtgcgctt gcaggagttt gattacccgg cggcgagcgc gattgcttcg
    21481 gtgatcctcg cggcatccct gctgctgctg ttctcaatta acactctgca aagtcgcttt
    21541 ggtcggcgtg tggtaggtca ttaatggcgg aagttaccca attgaagcgt tatgacgcgc
    21601 gcccgattaa ctggggcaaa tggtttctaa ttggcatcgg gatgctggtt tcagcgttca
    21661 tcctgctggt gccgatgatt tacatcttcg tgcaggcatt cagcaagggg ctgatgccgg
    21721 ttttacagaa tctggccgat ccggacatgc tgcacgccat ctggctgacg gtgatgatcg
    21781 cgctgattgc cgtaccggta aacctggtgt tcggcattct gctggcctgg ctggtgacgc
    21841 gctttaactt ccctggacgc cagttactgc tgacgctact ggacattccg tttgccgtat
    21901 cgccggtggt tgccggtctg gtgtatctgc tgttctacgg ctctaacggc ccgctgggcg
    21961 gctggctgga tgaacataat ctgcaaatta tgttctcctg gcccggaatg gtgctggtca
    22021 ccatctttgt gacgtgtccg tttgtggtgc gcgagctggt gccggtgatg ttaagccagg
    22081 gcagccagga agacgaagcg gcgattttgc ttggcgcgtc tggctggcag atgtttcgcc
    22141 gcgtcacatt gccgaacatt cgctgggcgc tgctttatgg cgtggtgttg accaacgccc
    22201 gcgcaattgg cgagtttggc gcggtgtcgg tggtttccgg ctcgattcgc ggcgaaaccc
    22261 tgtcgctgcc gttacagatt gaattgctgg agcaggacta caacaccgtc ggctccttta
    22321 ccgctgcggc actgttaacg ctgatggcga ttatcaccct gtttttaaaa agtatgttgc
    22381 agtggcgcct ggagaatcag gataaacgcg cacagcagga ggaacatcat gagcattgag
    22441 attgccaata ttaagaagtc gtttggtcgc acccaggtgc tgaacgatat ctcactggat
    22501 attccttcag gtcagatggt cgcgttgctg gggccgtccg gttccgggaa aaccacgctg
    22561 ctgcgcatta tcgccgggct ggagcatcaa accagcgggc atattcgctt ccacggcacc
    22621 gacgtcagcc gcctgcacgc gcgcgatcgc aaggtcggtt tcgtgttcca gcattacgcg
    22681 ctgttccgtc atatgacggt gttcgacaat atcgcttttg gcctgacggt gctgccacgt
    22741 cgcgagcgcc cgaatgccgc agccatcaaa gcgaaagtga caaaattgct ggagatggtg
    22801 cagcttgctc atctggcaga tcgttatccg gcacagcttt ccggcggcca gaaacagcgc
    22861 gtggcgctgg cgcgcgcgct ggctgtagaa ccgcaaattc tgctgcttga tgaaccgttt
    22921 ggcgcgctgg atgcgcaggt gcgtaaagag ctgcgtcgct ggctgcgtca actgcatgaa
    22981 gagctgaaat tcaccagcgt gttcgtgacc cacgaccagg aagaggcgac cgaagtagcc
    23041 gatcgtgtag tggtgatgag tcaggggaat attgaacagg ctgatgcgcc gaatcaggta
    23101 tggcgcgaac cagcgacccg ttttgtactc gaatttatgg gcgaagtgaa ccgcctgcag
    23161 ggaaccattc gcggcgggca gttccatgtt ggcgcgcatc gctggccgct gggctacaca
    23221 cctgcgtatc agggtccggt ggatctcttc ctgcgcccgt gggaagtgga tatcagccgc
    23281 cgtaccagtc tcgattcgcc gctgccggta caggtactgg aagccagccc gaaaggtcac
    23341 tacacccaat tagtggtgca gccgctgggg tggtacaacg aaccgctgac ggtcgtgatg
    23401 catggcgacg atgccccgca gcgtggcgag cgtttattcg ttggtctgca acatgcgcgg
    23461 ctgtataacg gcgacgagcg tatcgaaacc cgagatgagg aacttgctct cgcacaaagc
    23521 gcctgatagg ttgagtgaat gttaaacgcc cggaggcgct tcccgcgatc cgggcttttt
    23581 aatggcaagg tttgtaacct gtagacctga taagacgcgc aagcgtcgca tcaggcaaca
    23641 ccacgtatgg atagagatcg tgagtacatt agaacaaaca ataggcaata cgcctctggt
    23701 gaagttgcag cgaatggggc cggataacgg cagtgaagtg tggttaaaac tggaaggcaa
    23761 taacccggca ggttcggtga aagatcgtgc ggcactttcg atgatcgtcg aggcggaaaa
    23821 gcgcggggaa attaaaccgg gtgatgtctt aatcgaagcc accagtggta acaccggcat
    23881 tgcgctggca atgattgccg cgctgaaagg ctatcgcatg aaattgctga tgcccgacaa
    23941 catgagccag gaacgccgtg cggcgatgcg tgcttatggt gcggaactga ttcttgtcac
    24001 caaagagcag ggcatggaag gtgcgcgcga tctggcgctg gagatggcga atcgtggcga
    24061 aggaaagctg ctcgatcagt ttaataatcc cgataaccct tacgcccatt acaccactac
    24121 cgggccggaa atctggcagc aaaccggcgg gcgcattacc cattttgtct ccagcatggg
    24181 aacaaccggc actatcaccg gcgtttcacg ctttatgcgc gaacaatcca aaccagtaac
    24241 gattgttggc ctgcagccgg aagaaggcag cagtattccc ggcattcgcc gctggcctgc
    24301 ggaatatctg ccggggattt tcaacgcttc tctggtggat gaggtgctgg atattcatca
    24361 gcgcgatgca gaaaacacca tgcgcgaact ggcagtgcgg gaaggaatat tctgtggcgt
    24421 cagctccggc ggcgcggttg ccggagcact gcgggtggca aaagctaacc ctggtgcggt
    24481 ggtggtagcg atcatctgcg atcgtggcga tcgctacctt tccaccgggg tgtttgggga
    24541 agagcatttt agccaggggg cggggattta agattattgc gcaggcaccg ttttatccgt
    24601 ctgcgcaatt agcgcatgta aaaaaggctg tgcgttctca tcgctcttac cggggatctt
    24661 cacaatatac ggcttcccgg taatcgatga cgacgaagca accttatcaa taaactgctc
    24721 ggcagtgtca atgcggttac gggtattgcc cagcttcaga cgcagatgag aaaccgcttc
    24781 atcgcaggta tgttcatcac cgttgcgcac aaatatcaaa tcctttttct gtgctaatcc
    24841 ctccagcatg gcgttgatgc gggcttcttc atgggcagtt aacttcgcgt aagcggggag
    24901 tgtcattagt agtgtaatga ccagacaaat gattttcttc attgaattac cttatccttg
    24961 ttttgtgata gccgatttga ctgccagaat caggtaaagt ttcggttgga atacgatgag
    25021 gatcatcggc aaggagaaaa aattaaatga gtagtttgtt gttgtttaac gataagagca
    25081 gggcgctgca ggcggatatc gtcgccgtgc agtcgcaggt ggtttacggc agcgtgggca
    25141 acagcattgc cgtgcctgct atcaaacaga acggtctgaa tgtttttgcc gtgccgacgg
    25201 tattgctgag caatacgccg cattatgaca ctttctacgg tggtgcgatt ccggatgaat
    25261 ggtttagcgg ttatttgcgt gcgcttcagg agcgtgatgc gctgcgccag ctgcgcgctg
    25321 taaccacggg ctatatggga acggcatcgc aaatcaaaat ccttgccgag tggctgactg
    25381 cgctacgcaa agaccatcct gacctattga tcatggtcga tccggtgatt ggcgatattg
    25441 atagcggaat ttatgtcaaa cctgaccttc ccgaggcgta tcgacaatat ttactgccgc
    25501 tggcgcaggg aattaccccc aatatctttg agctggaaat cctgaccggt aaaaattgcc
    25561 gcgatctcga cagtgccatt gctgccgcaa aaagtctgct ttcagacaca ttaaaatggg
    25621 tggtgattac cagcgcctcc ggtaatgaag aaaatcagga gatgcaggtg gtggtggtca
    25681 gtgccgacag cgtgaatgtc atttcccatt cacgggtaaa aaccgacctg aaagggactg
    25741 gcgacctgtt ttgtgctcag cttatcagtg gtttgctgaa agggaaggcg ttaaacgatg
    25801 cggtgcaccg tgcggggttg cgcgtactgg aagtgatgcg ctacacccag cagcatgaga
    25861 gcgatgaatt gattttgccg ccgctggcgg aagcataaaa aatggcgccg atgggcgcca
    25921 tttttcactg cggcaagaat tacttcttga tgcggataac cggggtttca cccacggtta
    25981 cgctaccgga cagtttgatc agttctttga tttcgtccat gttggagata acaaccggag
    26041 tcagggtaga cttggctttc tcttccagta gcggcagatc aaattcaatg acagtatcgc
    26101 caactttcac gcgctgacct tcttcagcaa tacgcttgaa gccttcgcct ttcagttcaa
    26161 cggtgtcgat accgaagtgg acgaacagtt caacgccgct atcagattcg atagagaacg
    26221 cgtgattggt ttcaaagatt ttaccaatgg taccgtctac aggcgcgacc attttgttac
    26281 ccgttggttt gatagcaata ccatcaccaa cgattttttc cgcaaaaacg acatccggca
    26341 cgtcttcgat attgacgatc tcgccagaga gcggagcaat gatctcaata gttccggtat
    26401 ccttcttgtc gtcggaaacc agagatttca gtttatcgaa caaacccatg atcttctcct
    26461 aagcagtaaa ttgggccgca tctcgtggat tagcagattg ttttttcttc aatgaacttg
    26521 ttaaccagcg tcattaactc gtccgttgtc ggttgagcaa gagcctgctc tgctaacacc
    26581 ttcgcatctt cgaagttcgt gttacggata atcttcttaa tgcgcgggat agaaatggcg
    26641 ctcatagaga attcgtccag acccatcccc agcaacagaa gtgtagcacg ttcatcgcca
    26701 gcaagctcac cacacatgcc agtccatttg ccttcagcat gagaagcatc aataacttgc
    26761 ttgatcaagt tcagcacgga cggtgacatt ggttggtaaa ggtgtgaaat catatcatta
    26821 ccacggtcaa ctgccagagt gtactgcgtt aaatcattgg tgccgatact aaagaaatca
    26881 acttctttgg ctaaatgacg tgcaattgtt gcggcagccg gtgtttccac cattacgccg
    26941 atttcaattg actcgtcaaa cgctttacct tcgtcgcgca gttcctgttt gtagatttcg
    27001 atctctttgc gcagtgcacg cacttcttca acagagatga tcatcgggaa cataatgcgc
    27061 aatttaccga aagccgaggc acgcaggata gcgcggagct gatcgcgcag gatctcttta
    27121 cgatccatcg cgatacggat agcgcgccag ccgaggaacg ggttctcttc tttcgggaag
    27181 ttcatgtatg gcagctcttt gtcgccgccg atgtccatgg tacgaacgat aaccgcctgc
    27241 gagccacacg cttcagccac tgctttgtaa gcagcaaact gttcttcttc agtgggcagc
    27301 gcgtcgcggt ccatgaacag gaactcagta cgatacagac caacgccttc agcgccgtta
    27361 cgctctgcac cttcaacgtc acgaaccgta ccaatgttag cgcacacttc tacctggtga
    27421 ccgtccagcg taatagctgg cagatctttc agtttagcaa gctctgcttt ttcagaagcc
    27481 acttgctcct gaacagcgcg cattttatca ataacttcgt tggttggatt gacgtaaacc
    27541 tgattattta cggcatccag aatcagatag tcgtcatttt tcacctgaga ggtgacgcta
    27601 ccggtaccca cgatagcagg cagttccaga gaacgcgcca tgatagaggt gtgggaagta
    27661 cggccacccg cgtcggtgat gaaacccagc accttcttca ggttcagctg tgcggtttcg
    27721 gacggcgtca ggtcagcggc aaccagaatg acttcatcct gaatggcgct caggtcgata
    27781 atcttcaggc ccaggatgtt gcgcagcagg cgcttaccga tatcacgtac gtcagccgca
    27841 cgttctttca ggtattcatc atccagctct tccagggcag aagcctgacc ttcgataact
    27901 tcatgagcag ctgcgtcagc tgtcatgtgc ttatctttaa tcagggctat gatttcctgc
    27961 tccagctcct catcttcgag cagcataata tgcccttcaa agatggcttc tttttcttca
    28021 ccgaacgttt caccagcttt cgttttgatc gtttccagct gggctgatgc cttggcacga
    28081 ccgctcagaa aacgttcaac ttcctgatca acctggtcgg cagaaatttt tttccggtca
    28141 atgacaattt cgtcttcttt cagaagcaga gctttaccga aagcgatacc cggggatgct
    28201 aaaatgcctg aaatcataac cctaccttac ttgtgactga tttttaaaag aacccgggaa
    28261 attactcgag ttccgccatc agtttaacca gatgttcaac cgctttctgc tcgtcttcgc
    28321 cttctgcgga gatagtcaca acggtacctt gagtcaggcc cagagtctgc agtttaaaca
    28381 ggcttttcgc gctggcgctt ttgccgttgg aagtcacagt aatttcagaa gtgaagccct
    28441 tagcttcttt tacaaactgg gcagcagggc gggtgtgcag accgttcgga gcggtaatgg
    28501 taacttcttg ctggaacatt gtatttcccc aacttatagg tttagtgttg tggaactaaa
    28561 gtctagcctg gcggttcgac tttagcctgt attgttagcg ccagttttta acagacgcga
    28621 cgcacgaaga gcgcaataca gtccattgct ggcgggtgtt gaccgctgac gtttcgtacg
    28681 tcattaaaca ttatgccgct aaaggaagaa ttgaaccaaa tcataaaatc gattcagctt
    28741 tggcttttcc tgtaacgatt aatttcgcgc atcaaaataa ttagtctggt taaataccag
    28801 ttccgacagg ctgaatcaat gccaggaggg gcgaaagttt gaagcaggcc acaaaaaagc
    28861 acctaaaaag gtgctttttt acgcattttt aacaagctgg cattactgtt gcagttcttt
    28921 ctcagtgaag agatcggcaa acaatgcggt gcttaaataa cgctcacccg atgacggtag
    28981 aataaccaca atattcttgt tggtaaagct ttcatcttct tgtagtttca acgccgcagc
    29041 aacagctgct ccagaagaga tacctgcaag aataccttct tcttccatca gacgacgcgc
    29101 ggtagaaatc gcttcttcat tggtgatgcc aatgacttta tcgaccagct tgagatcgag
    29161 gttagctggg ataaaaccag cgccaatacc ctgaatttta tgcgggccag gtttaatctc
    29221 ttcaccagcc agtgcctggg cgataactgg agaatcggtt ggctcaacgg cgacagagat
    29281 aagatcggtc ttgcctttgg tgcctttaat gtagcggctg acgccagtca gcgtaccgcc
    29341 agtcccaacg ccagcaataa atacatcaac ctgaccgtcg gtatcttccc agatctccgg
    29401 accggtggtt ttttcgtgaa tttcagggtt tgccggattg ctgaattgtt gcagcagcag
    29461 gtatttctct ggattgctgg cgacaatttc ttctgctttt tggattgcgc ctttcatgcc
    29521 tttagcacct tccgtcagca ccaggtttgc acctaacgct ttcagcagct tgcggcgttc
    29581 aatactcatg gtttctggca tggtcagggt gagtttgtaa ccgcgagcgg cagctacata
    29641 ggccagtgca atcccggtat taccgctggt cggttcaacc agttcaacgc ctggtttcag
    29701 cacgccgcgc ttttcggcat cccaaatcat gttggcaccg atacggcact taacgctgaa
    29761 gctggggtta cgagattcca ccttcgccag aatgcgtccg ttaccgatgc gattcaggcg
    29821 aaccagcggc gtgtgaccga tagtcagcga gttatcttca aaaatcttac tcatggcctg
    29881 tccttaactg tatgaaattg ggatacaaca ggtagcatac ccgctcagag aatatgcgga
    29941 agtaaggatt tagcatatct atatacagaa gggaaataat gacatgcaag atggaataag
    30001 gggcggcata agccacccct gtttcacaca aacggtttac aaaataaccg gtagattgtt
    30061 accgccacat cgcgtgttta tcgcgatagc aatcgaccca catcgccgtc gcgccacaaa
    30121 cggcaacggg catgatgaac agattaagca gcgggatcat cgtaaacagg ctggttaaag
    30181 caccaaactg catattggtg attttgcgtg tgcgcagggc ggtgcgcatc tctttaaacg
    30241 gcactttgtg gttatcgaag gggtaatcgc aatactggat ggctaacatc caggcgctaa
    30301 acaggaacca cagtaccggc gcgacggttt gcccaatacc agggattaag taaagaatta
    30361 gcaatacaat tgcgcgcggc agataccagg caaatttttg ccattcgcgt ttcatgattc
    30421 gcggcacatc tttcatgata ccgaaaatcc cggtatctgg cggtgtagcg ccagtcaatc
    30481 gtgcttccag ttgttcagcc aataaaccgt taaacggagc ggcaatccag ttagcaatcg
    30541 tggagaagaa atagccaaac actaacagca cagagatgac agccagaggc cacaacagat
    30601 aactcagcca ttgtagccag tccggaacgt aactcatgag agtcgggatc cagacatcga
    30661 gctgtgtaaa gagccaccag aatgcgcccc ccatcaacaa aatattgacc agcagcggta
    30721 aaataacgaa acgccgaatc ccaggttgcg agacgagctt ccagccttgc gcaaaatagt
    30781 aaaaaccgct gcgtggggca gatgtgaatg atgaaaccat aatcaggatg agctcctttt
    30841 gaccaatccc aggaaaattc tgcgtatttt accgggtaat tgcgcaatgg acagttagga
    30901 tatgttcgaa aaaacagcaa aaagcacgat ttcatctatc tttgtgctgt gaaagttaat
    30961 agtgcacttg cacttgaggt aatcggcaaa tactcttagt gagtaaatgt ttgccgtggt
    31021 ggcaaggtgt tagaacaaca gagaatataa tgatgcagga tttgcgtctg atattaatca
    31081 ttgttggcgc gatcgccata atcgctttac tggtacatgg tttctggacc agccgtaaag
    31141 aacgatcttc tatgttccgc gatcggccat taaaacgaat gaagtcaaaa cgtgacgacg
    31201 attcttatga cgaggatgtc gaagatgatg agggcgttgg tgaggttcgt gttcaccgcg
    31261 tgaatcatgc cccggctaac gctcaggagc atgaggctgc tcgtccgtcg ccgcaacacc
    31321 agtaccaacc gccttatgcg tctgcgcagc cgcgtcaacc ggtccagcag ccgcctgaag
    31381 cgcaggtacc gccgcaacat gctccgcgtc cagcgcagcc ggtgcagcag cctgcctatc
    31441 agccgcagcc tgaacagccg ttgcagcagc cagtttcgcc acaggtcgcg ccagcgccgc
    31501 agcctgtgca ttcagcaccg caaccggcac aacaggcttt ccagcctgca gaacccgtag
    31561 cggcaccaca gcctgagcct gtagcggaac cggctccagt tatggataaa ccgaagcgca
    31621 aagaagcggt gattatcatg aacgtcgcgg cgcatcacgg tagcgagcta aacggtgaac
    31681 tgcttcttaa cagcattcaa caagcgggct tcatttttgg cgatatgaat atttaccatc
    31741 gtcatcttag cccggatggc agcggcccgg cgttattcag cctggcgaat atggtgaaac
    31801 cgggaacctt tgatcctgaa atgaaggatt tcactactcc gggtgtcacc atctttatgc
    31861 aggtaccgtc ttacggtgac gagctgcaga acttcaagct gatgctgcaa tctgcgcagc
    31921 atattgccga tgaagtgggc ggtgtcgtgc ttgacgatca gcgccgtatg atgactccgc
    31981 agaaattgcg cgagtaccag gacatcatcc gcgaagtcaa agacgccaac gcctgataca
    32041 cttaaggcaa attaactcct cttcgaaccc ccgcttgtcg ggggttttta gcattgatgg
    32101 tgcgatatgg aatcaatcga acaacaactg acagaactgc gaacgacgct tcgccatcat
    32161 gaatatcttt atcatgtgat ggatgcgccg gaaattcccg acgctgaata cgacaggctg
    32221 atgcgcgaac tgcgcgagct ggaaaccaaa catccagaac tgattacgcc tgattcgcct
    32281 acccaacgtg taggcgctgc gccgctggcg gctttcagcc agatacgcca tgaagtacca
    32341 atgctgtcac tggataacgt ttttgatgaa gaaagctttc ttgctttcaa caaacgtgtg
    32401 caggaccgtc tgaaaaacaa cgagaaagtc acctggtgct gtgagctgaa gctggatggc
    32461 cttgccgtca gtattctgta tgaaaatggc gttttagtca gtgccgcgac ccgtggcgat
    32521 ggcaccaccg gggaagatat cacgtctaat gtgcgtacta ttcgcgctat tccgctgaag
    32581 ctgcacggag agaatatccc ggcgcgtctg gaagtgcgtg gtgaagtgtt cctgccgcag
    32641 gcggggttcg aaaagattaa cgaagatgcg cgacgcacgg gcgggaaagt ttttgctaac
    32701 ccacgtaatg cggcagctgg ttcactgcgt cagcttgatc cgcgtattac agcgaagcga
    32761 ccgctcactt ttttctgcta tggcgttggt gttctggaag gtggcgagct gccggatact
    32821 catcttggcc gtttactgca atttaaaaag tgggggttgc cggtcagcga tcgggtaacg
    32881 ctttgtgaat cggcggaaga agtgctggcg ttctatcaca aagtggaaga agaccgcccg
    32941 acgctgggct ttgatatcga cggcgtggtg attaaggtca actcactgga acagcaggag
    33001 cagcttggct ttgtcgcgcg tgccccgcgc tgggcggtag cgtttaaatt cccggcgcag
    33061 gagcagatga cctttgtgcg tgacgtcgag tttcaggttg ggcgtactgg cgcgattacg
    33121 cctgttgcgc gtctggaacc tgtccatgtt gctggcgtgc tggtgagtaa cgcaacctta
    33181 cacaatgcgg atgaaatcga acgtcttggt ttacgcattg gcgataaagt ggtgattcgc
    33241 cgcgctggcg acgtgatccc gcaggtggtt aacgtcgtgc tttctgaacg cccggaagat
    33301 acccgtgagg ttgtattccc gacgcattgt ccggtatgtg gttctgacgt tgagcgtgtg
    33361 gaaggtgaag cggttgcccg ctgtaccggt ggcctgattt gcggtgcgca gcgtaaagag
    33421 tcgctgaaac actttgtttc ccgccgtgcg atggatgttg acggaatggg cgacaaaatc
    33481 atcgatcagc tggttgaaaa agaatatgtc catactccgg cagatctgtt caaactcacc
    33541 gcaggcaaac tgaccggact ggagcgtatg gggccaaaat cggcacaaaa cgtggttaac
    33601 gcgctggaaa aagcgaaaga aaccaccttt gctcgcttcc tctatgcact tggcatccgt
    33661 gaagtcggcg aggccaccgc agcaggtctg gcggcatatt tcggcacgct ggaagcgctg
    33721 gaagccgctt cgattgaaga gctgcaaaag gtgcctgatg ttggcattgt cgttgcatcc
    33781 cacgttcaca acttctttgc cgaagaaagc aaccgcaatg tcatcagcga gctgttggcg
    33841 gaaggtgttc actggcctgc gccgatcgtt atcaacgcgg aagagattga cagcccgttt
    33901 gctggtaaaa ccgtggtgct tacgggcagc ttaagccaga tgtcgcgtga tgacgctaaa
    33961 gctcgactgg ttgaactggg cgcgaaagtc gcgggcagcg tgtcgaagaa aaccgatctg
    34021 gtgattgccg gagaagcggc aggttctaaa ctggcgaaag cgcaggaact gggcattgaa
    34081 gtcatcgacg aagcggaaat gctgcgtttg ctgggtagct gagatggaaa aagagcagct
    34141 gattgaaata gccaatacga taatgccgtt tggcaaatat aaagggcgtc gcttaatcga
    34201 cctgccggag gagtatctgc tgtggtttgc ccgcaaagat gaattcccgg cagggaagct
    34261 cggtgagcta atgcaaatca cgctgctgat taaaaccgag gggctgacgc aactggtcca
    34321 gcccctgaag cgtccgcttt aagctttatc ggcgctgctt tcctgctgtg cctgtaactg
    34381 ttcggtctgg cgtttgtatc gacgcgccag caccgcacag accatcaatt ggatctgatg
    34441 gaaaatcatt aggggcagca ccatcatacc gatcaccgat gtggggaaca gaatgtttgc
    34501 catcgggatg ccatttgcca gactcttttt cgaaccacaa aagacgatag taatttcatc
    34561 tgccttattg aagcccagtc ggcgtgccat aaagacgtta actacaatca cgatagccag
    34621 aagaacgcag ctgaccacca cgataaacag caatgatccc cagccaactt tatgccagat
    34681 accattaacg acggcttcgc tgaatgctgt ataaaccacc aacagaatgg acgtctggtc
    34741 agttttcgca atccattttt tattgcgcga cacccagtca ccaatccacg gccgggaaag
    34801 atgccccaac acaaacggca gcagcagttg cagcataatt ttaccgacct gctcaaggct
    34861 gccccctgca ccgtgaacat tcatcaccag accaaccagc aatggtgaaa ggaaaatccc
    34921 cagcaggctg gatgctgacg cagaacaaac cgccgccgcg acgttaccgc ccgccattga
    34981 cgtgaaggcg attgcagact gcacggtagc cgggagaatg cacaagtaga gaaaaccgga
    35041 gtagagcatc gggtcgacat ttaccggttt ccaccaggca aacagtacac ccagaatcgg
    35101 aaacagcacg aaggtgctgc aaattaccca caaatgcagt cgccagtgac caccgccagc
    35161 aataatcgcc tcacgcgaca acttcgcgcc gtgcataaag aacagcaggg caatcgctgc
    35221 ggtggtcaga ttttcaaaga aggggacgaa atcgcctctg gccggaaaga aagaggccag
    35281 caacaccacc gtgatcaggg ttaaggtgaa aggatcgagg atacgaaaaa gtttcataaa
    35341 gactccagat gatcgatggt tctattgtgc ttcttccagt ttgagaaata aaattgattt
    35401 attgcatcca ttcatgaaga aaacagatga attattcttt aaaacaatta aaggttttcg
    35461 tcacagtagc gcaggagaaa agttttagtc gtgcaggaga gcgtatcggc ctgagccagt
    35521 cggcagtgag tcacagtgtg aaggaactgg aaaatcatac tggtgttcgc ctgctggaca
    35581 gaaccacgcg tgaagtggtg cttacagatg cagggcagca gctggctttg cgtcttgagc
    35641 gactgctgga tgaactgaac agcacgttgc gcgataccgg gcgtatgggg caacaactga
    35701 gcggaaaagt tcgggtcgct gccagccaga ccatttccgc gcatcttatt ccgcaatgca
    35761 ttgctgaaag ccatcgccgc tatccagata ttcagtttgt cctgcacgat cgtccgcagc
    35821 agtgggtgat ggaaagtatt cgccagggag atgtcgactt tggcatcgtc atcgatccag
    35881 gcccagtggg cgatctgcaa tgtgaagcga ttctttccga gcctttcttt ctgctttgcc
    35941 atcgcgatag cgctttggcc gtcgaagatt atgtaccctg gcaggcatta cagggagcga
    36001 aattggtgtt gcaggattac gcgtcaggca gccgaccgct gattgacgca gcgctggcgc
    36061 gcaatggtat tcaggcgaat attgtgcagg agattggaca tccggcgacg ctgttcccga
    36121 tggtagccgc aggcatcggc atcagtattc ttcccgcact ggcgctacca ttgcctgaag
    36181 gtagcccact ggtggtaaag cgcattacgc cggttgttga acgccagttg atgctggtgc
    36241 gcaggaagaa ccgttcactt tccaccgccg ctgaagcgtt atgggacgtg gtacgagacc
    36301 agggcaatgc gctaatggct ggtcgggaag gggatccgtt gtatcagata tagagtgaat
    36361 tcatactgcc gggagccccg gcagtaatca atcagaagtt aaatttgatg gagtagatta
    36421 tcgcatcctg gtaaaaatct tcgcccagtt tgtcatccgc atatcgatac tgaatccccg
    36481 tagtaatgtg tgatgttgca ttccaccaca gtgcaactgc gccattcagc ccttctttac
    36541 cgccattacc cgctgcatag gtagcgtcac ggtcaaactc gtactcgttc cagttggtaa
    36601 gagtgaattt ctcgctgccc agcataaagt tgtaacctgc aacccagccg gcgacatagc
    36661 cgttgtcgcc agtgtaatag gtttgatctg tataacgctt agcaaagaac ggtttgaacc
    36721 accaaccgct gccggtgaaa ttgtagccga taccgtacag gaacatatcg tcgtggaaat
    36781 tcacgcgatt cgcagaaccg taggtgccat acgcatgcaa gtagagatta aatccggtgt
    36841 cacccaggta aatacggttg gtatttttaa aggtataacg ctgctcgcta cctggtttat
    36901 tatggcgacc gttatagaag ttttcccagt cgaagaaccc gtacatttct ccccaactaa
    36961 agttagcgcc accttcaaat tcaagataac caaaatcatc tttgtgtgat ttggtcgacg
    37021 atttttcggt agtccggctg gtccagtcca gataatgcac gccgatatct gcaaaaccgc
    37081 ctttaaattc tgcatgagat acaacaggta tcgctaatat agaggaaagt gtcagagtta
    37141 aaagatgttt tttcatagca taattcccta tgccgatcgt tattagtaga ttgcttaata
    37201 aagtggtttg ctaaaaacga catattgtta atgcaaagga attaatatcg ccaatacagt
    37261 ttttaccgtg gattaataga gaaaagatat aaatccatcg atatcgaatc gctattgaat
    37321 gcgtgagaaa agtgtattgg taagagccgg gacaacctcg ctataaaaag gcgttagatt
    37381 ccaccctaca gaaaaaggat atgtatgtct caggttcaat tttctcataa cccgctgttt
    37441 tgcatagata ttatcaagac ttataaacct gatttcacgc cacgagtggc ctttatttta
    37501 ggttccgggc tcggcgcgct ggccgatcag attgagaacg ctgtcgcaat ttcctacgaa
    37561 aagctgccgg gcttcccggt aagtacggta catggtcatg cgggggagtt agtgctgggc
    37621 catctccagg gcgtaccggt ggtatgtatg aaaggtcgcg gacatttcta cgaaggtcgt
    37681 ggaatgacca ttatgactga cgcaattcgt acctttaagc tgctgggctg cgagctactg
    37741 ttctgcacca atgcggcagg ctcgctgcgt ccggaagtgg gggcaggcag cctggtcgca
    37801 ttgaaagatc atatcaacac catgcccggt acgccgatgg tgggtcttaa cgatgatcgt
    37861 tttggagagc gcttcttctc actggcaaat gcctacgatg cggaataccg cgcactgtta
    37921 caaaaagtgg cgaaagaaga gggtttccct ctgacggagg gcgtgttcgt ctcgtatccg
    37981 gggccgaatt tcgagactgc ggcggaaatt cgcatgatgc aaattattgg tggggatgtt
    38041 gttggtatgt ctgtggtgcc tgaggttatt tcagctcgcc attgcgacct taaagtcgtt
    38101 gcggtctctg cgattaccaa tatggcggaa ggtttaagcg atgtgaaatt gtcacatgcg
    38161 caaacgctgg cggctgcgga gctctctaag cagaacttca tcaaccttat ttgtggcttt
    38221 ctgcgcaaaa ttgcctgaga aaaacaataa gaaacggcct cgcgggtgag gccaaatgat
    38281 gcgacaagga aacgattatg agcatcgcga tgcgcttaaa ggtaatgtcc tttttgcaat
    38341 attttatctg ggggagctgg ctggttaccc tcggctctta catgattaat acccttcatt
    38401 tcaccggcgc taatgttggc atggtttaca gttccaaagg gatcgccgcg attattatgc
    38461 ctggtataat ggggatcatc gcagacaaat ggctgcgcgc agaacgtgca tacatgctgt
    38521 gtcacctggt gtgtgcgggc gtactttttt atgcggcatc cgtaactgat ccggatatga
    38581 tgttttgggt gatgttagtc aatgcgatgg cgtttatgcc gactattgcg ttatcgaaca
    38641 gcgtctctta ttcctgtctt gcccaggcag ggcttgaccc ggtgaccgct ttcccgccca
    38701 ttcgcgtttt tggtacggtg gggttcattg tcgcgatgtg ggcagtaagc ctgctgcatc
    38761 tggaattgag tagtctgcag ctgtatatcg cgtccggtgc gtcattgctg ctgtcggctt
    38821 atgcgctgac tttgccgaag attccggttg cggagaaaaa agcgaccaca tcgcttgcca
    38881 gcaagctggg tctggatgcc ttcgtgctgt ttaaaaatcc acgcatggcc atctttttcc
    38941 tctttgccat gatgctgggt gcggtactgc aaattaccaa cgtttttggt aatccgttcc
    39001 tacatgattt cgcccgtaac ccggagtttg ctgacagttt tgtggtgaaa tatccctcca
    39061 ttttactgtc agtttcacag atggcagaag tgggctttat actgactatc ccattctttt
    39121 taaagcgatt tggcattaaa accgtcatgc tgatgagtat ggtggcctgg acgctgcgct
    39181 ttggcttctt cgcctatggc gatccgtcaa caaccggatt tattttgctg ctgctgtcga
    39241 tgattgttta tggctgtgca ttcgatttct tcaatatttc tggttcggta tttgtcgaac
    39301 aggaagttga ttccagcatt cgtgccagcg cgcaggggct ctttatgacc atggtaaatg
    39361 gtgtcggcgc atgggttggc tcgattctga gtggcatggc agtagattac ttttcggtgg
    39421 atggcgtaaa agactggcaa actatctggc tggtgtttgc aggatatgct ctttttctcg
    39481 cagtgatatt tttctttggg tttaaatata atcatgaccc tgaaaagata aagcatcgag
    39541 cg
//
LOCUS       DAZFRJ010000020        38095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000020 DAZFRJ010000000
VERSION     DAZFRJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.020"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..296
                     /locus_tag="ACHKUN_000967"
     CDS             <1..296
                     /locus_tag="ACHKUN_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659276.1"
                     /translation="IKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSAHNMTMTTYY
                     KYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNTLQKC"
     gene            complement(351..1328)
                     /locus_tag="ACHKUN_000968"
     CDS             complement(351..1328)
                     /locus_tag="ACHKUN_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO6659277.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(1345..2274)
                     /gene="flgL"
                     /locus_tag="ACHKUN_000969"
     CDS             complement(1345..2274)
                     /gene="flgL"
                     /locus_tag="ACHKUN_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6659278.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(2289..3665)
                     /gene="flgK"
                     /locus_tag="ACHKUN_000970"
     CDS             complement(2289..3665)
                     /gene="flgK"
                     /locus_tag="ACHKUN_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6659279.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(3847..4146)
                     /locus_tag="ACHKUN_000971"
     CDS             complement(3847..4146)
                     /locus_tag="ACHKUN_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO6659280.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(4146..5246)
                     /locus_tag="ACHKUN_000972"
     CDS             complement(4146..5246)
                     /locus_tag="ACHKUN_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6659281.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(5261..5926)
                     /gene="flgH"
                     /locus_tag="ACHKUN_000973"
     CDS             complement(5261..5926)
                     /gene="flgH"
                     /locus_tag="ACHKUN_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6659282.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(6009..6794)
                     /gene="flgG"
                     /locus_tag="ACHKUN_000974"
     CDS             complement(6009..6794)
                     /gene="flgG"
                     /locus_tag="ACHKUN_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6659283.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(6873..7610)
                     /locus_tag="ACHKUN_000975"
     CDS             complement(6873..7610)
                     /locus_tag="ACHKUN_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO6659284.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(7610..8812)
                     /gene="flgE"
                     /locus_tag="ACHKUN_000976"
     CDS             complement(7610..8812)
                     /gene="flgE"
                     /locus_tag="ACHKUN_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6659285.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(8836..9549)
                     /locus_tag="ACHKUN_000977"
     CDS             complement(8836..9549)
                     /locus_tag="ACHKUN_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO6659286.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(9549..9980)
                     /gene="flgC"
                     /locus_tag="ACHKUN_000978"
     CDS             complement(9549..9980)
                     /gene="flgC"
                     /locus_tag="ACHKUN_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6659287.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(9983..10318)
                     /locus_tag="ACHKUN_000979"
     CDS             complement(9983..10318)
                     /locus_tag="ACHKUN_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO6659288.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            10400..11137
                     /gene="flgA"
                     /locus_tag="ACHKUN_000980"
     CDS             10400..11137
                     /gene="flgA"
                     /locus_tag="ACHKUN_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6659289.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            11218..11496
                     /gene="flgM"
                     /locus_tag="ACHKUN_000981"
     CDS             11218..11496
                     /gene="flgM"
                     /locus_tag="ACHKUN_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6659290.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            11509..11937
                     /gene="flgN"
                     /locus_tag="ACHKUN_000982"
     CDS             11509..11937
                     /gene="flgN"
                     /locus_tag="ACHKUN_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO6659291.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            12064..12993
                     /locus_tag="ACHKUN_000983"
     CDS             12064..12993
                     /locus_tag="ACHKUN_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO6659292.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            13172..15616
                     /locus_tag="ACHKUN_000984"
     CDS             13172..15616
                     /locus_tag="ACHKUN_000984"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6659293.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(15734..16129)
                     /locus_tag="ACHKUN_000985"
     CDS             complement(15734..16129)
                     /locus_tag="ACHKUN_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO6659294.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(16132..16566)
                     /gene="fliJ"
                     /locus_tag="ACHKUN_000986"
     CDS             complement(16132..16566)
                     /gene="fliJ"
                     /locus_tag="ACHKUN_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6659295.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(16569..17906)
                     /gene="fliI"
                     /locus_tag="ACHKUN_000987"
     CDS             complement(16569..17906)
                     /gene="fliI"
                     /locus_tag="ACHKUN_000987"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6659296.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(17899..18609)
                     /gene="fliH"
                     /locus_tag="ACHKUN_000988"
     CDS             complement(17899..18609)
                     /gene="fliH"
                     /locus_tag="ACHKUN_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6659297.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(18612..19622)
                     /locus_tag="ACHKUN_000989"
     CDS             complement(18612..19622)
                     /locus_tag="ACHKUN_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6659298.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVGRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(19600..21246)
                     /gene="fliF"
                     /locus_tag="ACHKUN_000990"
     CDS             complement(19600..21246)
                     /gene="fliF"
                     /locus_tag="ACHKUN_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6659299.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(21251..21592)
                     /locus_tag="ACHKUN_000991"
     CDS             complement(21251..21592)
                     /locus_tag="ACHKUN_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6659300.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(21607..22593)
                     /locus_tag="ACHKUN_000992"
     CDS             complement(21607..22593)
                     /locus_tag="ACHKUN_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO6659301.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            22980..23831
                     /locus_tag="ACHKUN_000993"
     CDS             22980..23831
                     /locus_tag="ACHKUN_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6659302.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            23824..24195
                     /locus_tag="ACHKUN_000994"
     CDS             23824..24195
                     /locus_tag="ACHKUN_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6659303.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            24192..24944
                     /gene="fliP"
                     /locus_tag="ACHKUN_000995"
     CDS             24192..24944
                     /gene="fliP"
                     /locus_tag="ACHKUN_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6659304.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            24947..25219
                     /locus_tag="ACHKUN_000996"
     CDS             24947..25219
                     /locus_tag="ACHKUN_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO6659305.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            25221..26003
                     /gene="fliR"
                     /locus_tag="ACHKUN_000997"
     CDS             25221..26003
                     /gene="fliR"
                     /locus_tag="ACHKUN_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6659306.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            25993..27132
                     /gene="flhB"
                     /locus_tag="ACHKUN_000998"
     CDS             25993..27132
                     /gene="flhB"
                     /locus_tag="ACHKUN_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO6659307.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            27116..29209
                     /gene="flhA"
                     /locus_tag="ACHKUN_000999"
     CDS             27116..29209
                     /gene="flhA"
                     /locus_tag="ACHKUN_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6659308.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            29314..29592
                     /locus_tag="ACHKUN_001000"
     CDS             29314..29592
                     /locus_tag="ACHKUN_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO6659309.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            29585..29941
                     /locus_tag="ACHKUN_001001"
     CDS             29585..29941
                     /locus_tag="ACHKUN_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO6659310.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(29998..30771)
                     /gene="yafL"
                     /locus_tag="ACHKUN_001002"
     CDS             complement(29998..30771)
                     /gene="yafL"
                     /locus_tag="ACHKUN_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6659311.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            30957..31217
                     /gene="dinJ"
                     /locus_tag="ACHKUN_001003"
     CDS             30957..31217
                     /gene="dinJ"
                     /locus_tag="ACHKUN_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO6659312.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            31205..31492
                     /gene="yafQ"
                     /locus_tag="ACHKUN_001004"
     CDS             31205..31492
                     /gene="yafQ"
                     /locus_tag="ACHKUN_001004"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO6659313.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            31648..32388
                     /gene="dpaA"
                     /locus_tag="ACHKUN_001005"
     CDS             31648..32388
                     /gene="dpaA"
                     /locus_tag="ACHKUN_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO6659314.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(32359..33126)
                     /gene="yafJ"
                     /locus_tag="ACHKUN_001006"
     CDS             complement(32359..33126)
                     /gene="yafJ"
                     /locus_tag="ACHKUN_001006"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO6659315.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(33332..33910)
                     /gene="lpcA"
                     /locus_tag="ACHKUN_001007"
     CDS             complement(33332..33910)
                     /gene="lpcA"
                     /locus_tag="ACHKUN_001007"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO6659316.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            34150..36594
                     /gene="fadE"
                     /locus_tag="ACHKUN_001008"
     CDS             34150..36594
                     /gene="fadE"
                     /locus_tag="ACHKUN_001008"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO6659317.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(36637..37083)
                     /gene="ivy"
                     /locus_tag="ACHKUN_001009"
     CDS             complement(36637..37083)
                     /gene="ivy"
                     /locus_tag="ACHKUN_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO6659318.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            37264..38031
                     /gene="yafV"
                     /locus_tag="ACHKUN_001010"
     CDS             37264..38031
                     /gene="yafV"
                     /locus_tag="ACHKUN_001010"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO6659319.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
BASE COUNT         8130 a        10542 c        10442 g         8981 t
ORIGIN      
        1 gaataaaaat atttcacctt agttctggaa aagaaaataa ttccgtaccg ttactgtcac
       61 aaaccctcgc accaggaaaa gacaaactcg ataatttatt gtcagcgcat aatatgacaa
      121 tgacaaccta ttataaatat gttcgtaacc gactggaaag tgatattgtt ctgcgcaacc
      181 agtgcaatgg tagctggcaa ttaactttta atgtggaagg ctggcagaac agcgatatta
      241 atagcgcgat gtatcagaat ttagagaagt tgttaaatac tttgcagaaa tgctgatgag
      301 tgccaatata tgccggatgc gacgctaaac gcgtcttatc cggctgataa ttaccccaat
      361 aaattcctca ccgtctggct ggaaattcgc gcctgaccat tcaccgcctg caataaaacc
      421 tgatagttat gattcgcgct atccagcacg ccgccgaggt tttccgacgc ctgcaccgcg
      481 ttttccgttt gcgggaagcg cgacatgccg tcgatcaact gccgcgcctt ttcctgctgt
      541 accgccatct gcgcacgctg ctgattaatg ctttctaacg tctgagaaat tcccgcaccg
      601 ccctgttgca ggctttgagc taaacggtct gcctcagacg gctcggcaaa agttttcagc
      661 ggcataaacg ccgacttatc gccatcggtg cgcacgctta aggtacgttc gatatggggc
      721 cactgttttt cggtggtgga gaaactgatg ccgtccgctt gctgatgtag ctgcacgccc
      781 acgcggcgca gggcgttggt cagccgcgtc tgatactgcg cggcgctgtc atcctcagac
      841 agcatcaccg ccgaaagctg agtctgccgc ccgtcagaga cgctgaacat gcgcgttccc
      901 ggcgtgggat tgtgtaccag attcgccaga tccggcgagt gaaaggtcac ccgcgcttcg
      961 ccctgtaaca ccggctgcaa ctggcggtcc accgcaccgc cggaaagcgc ggtgcgtttg
     1021 tccagcatct gcatcagcgc cgtattctgc gcctgcccgc ctttacgctg gctgtgacga
     1081 tagtcgagca actgctgctc cagttgcccg agataatgat ccgcctgctg caacgtggtg
     1141 agctggtcgt taagctgcac gctgtaacgc tgcgggcgag tggtaatcag cggcgaggcc
     1201 ggatattcac tcgccgtcgc cgggagtttt tgccgcaccg cctgagtggg cgcaacggtt
     1261 gacgacgata ccgcagcgct atgcgcgccg ccggtggcga gtgatgaaga tgtaagtccg
     1321 acctgcatac tgttcctgaa agagttagag aacgctgaac aaattcaact cactcacttt
     1381 cgagtaagtc ttgagggtaa cttccattgc cacttccagt ccggtaaagg tgatcgacgc
     1441 cgggccgtaa tcgagatcct gtaatgaacc aatcagctca tcattagagg tggagatatc
     1501 ggtctgcgca tcgctcagca tcgacatggt attttgcgtc tcgccgagtg acgcaatcga
     1561 ggcgttgagg tcgtccgagg cggtgtccac cacatcaacg gcattctgaa tatcgctctg
     1621 cacctgcgga tctgccgggt cgacgtccgg gttttgcaac tcctgggata gggaattcag
     1681 ggtgttgagc acgtctaaat tgctgccaaa gaaatcactc gccgccacgt tggtgtccac
     1741 ttccacgccg ttcgacaccg tggtctggcg gtggtcgctg ttaccctgat agctgtagct
     1801 gtcggtgacg ccatcgccgt catcgtccac tgccacaatc ggcggctgat cgttgatggt
     1861 gccgccaaac acgtagtgcc cgtcttcgtc ctgataattg agcgccgcca ccattgattc
     1921 ggtgagcgac tcaatatcct gcccgaggct tgcgagtgaa tcggcggtat tggtgccatt
     1981 cgccgcttcc agcaggtcgt cgcgcaccgc cagcaggctg ttgttgacgc catcaaggat
     2041 cgactcctgc tggcttaagc ccgccgacgc ggagtcgata ttgctctgat actgctcaat
     2101 cgccgactgc tggcggttta actgggtgat tcgcgtggcg gcgatcgggt catccgacgg
     2161 ctgcaaaatc tgcttgccgg ttgccatctg ctcgacaaca tgcgccagat cgccagagag
     2221 attattaaaa ctttgcgtca tcgagacgta ggtttgttgg gtagttactc gcattttcgc
     2281 gctcccgctt agctgcacat ttccagcacc gaatcgaaaa tctcggcccc ggcggaaatg
     2341 actttcagat tggcttcata aatttgttga taggtgatca ggttcaccgc ctcttcgtcc
     2401 atgctgacgc cgctgacgct gctctgctgg ttttgcgctg cggaatagac attggacgcg
     2461 gcatcgactt ccgtctggtt ttgctgactg taaatgccga tattgctgat gattgacgag
     2521 cacgcctgcc cgaccgtcac gctgccgagg ttggctattt ccagcggttc ggtggagata
     2581 ttgatcagcg cctgcaggtt gtcgctgttg ccgctttcgt ccggtgaact ggaaaacgcc
     2641 agctcatcgg cggtgatatt cgggttaacg gtcagcgggc catcggcatt gctggcgtca
     2701 taaataaaca gcggctcgcc ggggttaccg ttgagatcgt agccctgcgc cagctggttg
     2761 ttgaccgcat cggcgaactg cgacgccatg ctgttgatgg tgtcggtcag cggcgtcagc
     2821 acatcgtttt gataatcaaa cagtgcgcct aatgaaccgc cggtgtcggt ggtcatcgtc
     2881 gaggtggtgc cagcgaaggt cagcgacatg gtcgacgtgc catcggcgtt ggtttccagc
     2941 gcgatggtcg aactttgctg cccactcacc agcggctgac cgtttttcag ggtaacgttg
     3001 tagttgccct ggtcgtcgat attgacctgc acatccatca tcccgctcag ttcttccacc
     3061 atctggtcgc gggcgtcgta cagcgcggag gcgttatcgc cattggcttc agcctgagcg
     3121 atttgctggt tatagctggc gatgccgctg gtgagcgtat tgatttgcga caccatcgcc
     3181 tgctgctggc tgatgatttc cgtgctttgc gagtcgatgt aatccagcgt gttatcaata
     3241 cgcaacgaca gcgccccggc ttcactgatc acctgctcgc gcagggcgga atcatcgggg
     3301 ctggtggttg cttcgttaag ggcggcgaag aagttatcga agccgccgct caggctgctg
     3361 ttatcgtcgc tcagtaccgc ttccagttgg ctgagatccc cctgctgggt gctgtaatag
     3421 ccgtaatcgc tggcggcata ccacacctga ttcacctgat actggttgga gacgcggcga
     3481 atgctgtcga cctgtacgcc gttaccggcg ctgttcggcg aaccgccgct ggcaccgatg
     3541 gtgctgatct ccgccacctg acgggtgtag cccgtggtca tggcgttggc ggtgttttgc
     3601 gccgtgacgt taagctccac ctgcgcggtt gatgcaccgc tgtagccgat gttaatcatg
     3661 tccatgttgg atccttaccg gtaaatcgtg ccggttaaaa gcggcgtaaa gttggggatt
     3721 cttaagttcc gtaaactggg tgccgaacgc cggatgcggc gtggacgcct tatccggcct
     3781 acatacgggc gactcgtagg cctgataaga cgcgacagcg tcgcatcgtg caccgaacgc
     3841 cgaagctcat ttatgcttcg gcgacaactg cttcacaatc atcgccgcaa tgccaatccc
     3901 gtgctgctgc gccagggtgt tacacaactc atcgtcataa aacccctgca tcatgcgcac
     3961 cgaatcgctg ttaaacgggt tatcttttga atcaaacaac tcgttggttt tccgcatctc
     4021 tttcagcatg ttgcgtaaaa aaatcgcctc aaactgttcg gcggcctgct tgatatcatt
     4081 ggcctgaacc ttcggcccca ttaacgcgtc gctgccgtca atcccgccgc tggcgttcac
     4141 tttcatcaga tcacctccag gtcggcatcc agcgcgcccg cctcatgcag cgcctgcaaa
     4201 atcgacataa tgtcgtccgg cgtcgcgcct aaactgttca gcgcattgac gagcgttttc
     4261 aggctgctcg actccggcaa actcaccacg cccggacggg catgattcac cgcgatattg
     4321 ctctgcggcg taaccgccgt gcgcccaccc gcaaaggcat tcggctggct gacgttgctg
     4381 gtttcgttaa tcgaaacggt caggctgccg tgcgacaccg ccgccgcgtg tagcgcaacg
     4441 ccatcgccca ttaccaccgt gccggtacgc gagttaaaca ccacgcgagc gcggattttc
     4501 tccgcctgca cctgcacatc gtccagttgc gacataaatg ccacccgcgc gccggggctg
     4561 gtgggcgcgc gcacggtaac gttggtggaa ctctgcgcgg tggcaatacc gccaaacgag
     4621 gcgttgatcg ccgccgcaat gttgttggcg tctttaaacg acgggcgttt caggttgagg
     4681 gtgatggtat cgcccatctg aaaatcgctg gggatctcgc gctccaccgt tgcgccgtta
     4741 ggaattaacc cggcggttgg cgtattaacc gttacgctgg aaccgctggc cccggtggcg
     4801 ttcatcccgc ccaccaccac gctgccctgt gccagggcgt aaacttcccc atcagcgccg
     4861 tgcagttggg tcagcagcaa agtgccgccg cgaatacttt tggcatcgcc aatcgacgaa
     4921 acggtgacat caatggtctg cccgcgcgag tacatcggcg gcagcgtggc gctcaccgcc
     4981 accgcggcga cgtttttcac cttcgggtcg attttattcg gcagttgcac gccaaactgg
     5041 cgcagcatat tggtgatggt ctggttggtg aatttgacct ggtttttatc accggtgccg
     5101 tcgaggccga ccaccaggct atagcccacc agttggtttt cgcgcacgcc ctgtacgttg
     5161 accagcgact ccagcgattg cgccagcgcc acaccaggca gcgccaggct cacggcgata
     5221 actgcggcct ttatccagtt ttgcattgca ctctccggca ttaaatcggg aacagaggat
     5281 ggttaaacag ccgcgtcagc cagcctgcgg cgttggcgtc gcttaacgcg ccgcgtccgg
     5341 cataggagat gcaggcatcg gcgatccgct gggaggaaac cgagttgtcg ttctggatgt
     5401 cgtcggcgcg caccaggccg cttaagcgga tgtactcatc gccctggttg agggtgagcc
     5461 atttctcgcc gcgaatttcc aggatgccgt tgggctgcac cgcatggacg gaaacggtga
     5521 tctccccgcg caggctgttc tgctgctggg aagtggcgct gccgtcaaag tcgcggttgg
     5581 cgtcgataga gccggaaagc ttatctttag tgtgactaaa aatggtcggt gcgccgatat
     5641 cgacggtgtt gcttttgccg aagttggttt tcgcctgttt gctcgactgg gtggattctt
     5701 cgaggatcac cgtcaggata tcgcccacgc gataagcccg ccgatcggcg gtcagcgacc
     5761 agttgtagcc agtttcaaat acaccgccag cacgcccgtc tgccggaggc ggtgcttcgg
     5821 ttttcggtgg cgcaaagtat gcgtcgtctt ttttgaccag aatggcctgc gattcacacc
     5881 cgctaagcag gggaagcagc gccaccagcc agagataatt tttcacgttc tgcctgttta
     5941 aatacagtga gttatgcgct tttgtaggtc ggataagacg cgtcagcgtc gcatccgaca
     6001 tttcggcctt acagcgtctg actgatgtac tgcaacatat cgtccgcagc cgacaccatt
     6061 ttggcgttca tctcgtaagc ccgttgcact gtaatcatcg ccaccatttc gttgacgata
     6121 tctacgttcg agccttcgag ggcattgtcc tgcaaagtgc caagcccatc ttcgcccggc
     6181 acaccttcgg tcggctgacc gctggcggcg gtttccagat acaggttgtc accctcagcg
     6241 gaaagcccag ccgggttagc gaagttaacc agcgtcagtt gccctaactc ggtcacgtcg
     6301 ctgtcgccag gaagaatcgc cgtcaccgtg ccgtcctcac caaacgcaac attcgtcgcc
     6361 cccgccggaa cgtcgatttc cggctgcaat ggcaagccct gtgagttggt cagtacgcca
     6421 tcggcgttca cctgcaaatt gccgtcgcgg gtataggcga tatcgccgtt ggccttttgc
     6481 acctgcaaaa atccctgccc cataatggcg acattcatcg cgttgtcggt ggtttcgacg
     6541 ttgccttcgg tgaaggtttt ctgggtgccg acaatgcgca cgccgctacc gaattgcagc
     6601 ccggtcggca tgatgttgtt ctggtcgagc atcgcgcccg gcgcttcctg ggtctggtag
     6661 aacagatcct gaaacatcac ccggtcacgc ttgaagccgg tggtgttaac gttggcgatg
     6721 ttgttggcaa tcgcgctcat ttcagcatcc tgcgccgaga ggccggtttt gctgatccat
     6781 aatgctgcgt tcatggtggt aaattccgtg ttatttttgg gccgcggttg ccggatgcgg
     6841 cgtgaacgcc ttatccggcc tacgatgtgc ggttacgagc cgcgcaataa gcggttgccc
     6901 gcgtcgctga tatcttcggc ggttttcatc atcttgatct gtgcttcgaa ctggcggttc
     6961 atggcgatgg acgacatcat ttcgcttacc gccgagacgt tgctgccctc gagaaaaccg
     7021 ccgctgactt tgatgttttc atcgcgctgg gccggaacgc cgtcggcagt caccagcatc
     7081 ccttcagcat ttttctccag gttagccacc gggatatcca ccagcttcag gcgatcgata
     7141 tccatcgtcg cggtcacatc gccgtcatcg ggcgtcaccg acagcgtgcc gtcgctgcca
     7201 aacgaagcaa tggcgttcgg cggcagaatg atcggcccgt tatcccccag caccagattg
     7261 ccgtcgatgg tcagttgccc ctggtcgtcc tgctgaatgt tgccgttgcg ggtatagact
     7321 tcgttaccgt ttttatcctg caccgcaatg taacccgcgc cctgaatcgc cacgtccagc
     7381 gggcgttcgg ttttctccgc aacacccgtg ctgtcgttca cgccgctggc cccttcctgc
     7441 gccatatagc gcgtgtcgaa gccgccgcct ttcaccttgt cgttggttgc cattgccata
     7501 tcggcgcgaa aaccattggt gttgacgttc gccaggttgt tggcgctaat ttgctgctca
     7561 tacagggtct gagaagcacc gctcagggcg gtataaatca ggcggtccat tacattgcct
     7621 ggaacagcgc gtcatcgagc tgcgtgctgg tggcgataac tttggtgttg gcctgatagt
     7681 tacgctgggc ggtcatcagg ttgaccagtt cgctggtgat atcgacgtta gagctttcga
     7741 gcacgcccga cgacagcgta ccgcaggtgc cggagccggg aacgccaatc agcggggtgc
     7801 cggattcgcc cgtttgtacc catgcggtgc cgctcactgc ctccaggccg ttttcattcg
     7861 ggaaagtcgc cagcaccacc tgcccctgca acatgcgctc gccgttgctg taggtggcgt
     7921 aaactttgcc atcgtcatca acctgaacac cgttttgcgt ggcggaggcg tagccgttgg
     7981 cggcgttggt ggtgaccgaa aattcagagc cgtattgcgt acaggtggag taatcgacgg
     8041 ttaagtcgac gggcgcggcg gcgtcggttt gaaattcaat ggtctgcggc gtggttggag
     8101 aggtcagctt cccggtgttc ggatcgaagg ttaacgtcgt cgccggaacg ccggtctgct
     8161 gctgaccgtc gaaggtgtac tgcacttccc aggtgttgtc gctggttttg gtgaagtact
     8221 ggcataccga atgttcgttg cctaatgagt catacaccgt ggtggtgtag ctgtcggtgt
     8281 acgaactgct gttagtcgcg tcgaacggaa cggtggtgcg atcgatagct tcatcgctgg
     8341 cgttgaagtt agcggtaaag gtcaggctgc tgctggcctg cgccgggata ttgccggttt
     8401 tgatctggat atcggtgacc gtaccggttt gcagagtgcc agtgtcatcg accggatagc
     8461 cctgcaaata agcgcccgag gcgttgacga tatagccgtt tttgtcggtt tcaaacgatc
     8521 cggcgcgggt ataggagata ttgcctgcgc tgtcacacat gacaaaaaat ccgtcgtcgt
     8581 taatcgctaa atcgagcgca ttgccggtgg agaccagcga accgccacgc gaaatacttt
     8641 gcgcggtgcc cgccacgctg acgcccatcg cctggcttcc ggcatacatg gcggaaaact
     8701 gggtggtcat cgacttatag cccaccgttc cggcgttagc gatgttgtta ctgatcccgt
     8761 ccagctgttc gttaacggca ttcagccccg ttgcggcaat ttcataactc attgtgtctt
     8821 cctgttaatt gattcttacg ccacatcact gtcggcatcg ggtacgccaa actggctaat
     8881 catggtgaac ggcacttcgc ccacgcctgc aacgttaagc accggggaac tgccgtcgag
     8941 cgggatgcgc acatcttcca cttcgccgga gacttcaatc ggcacctctt cttccccggt
     9001 attggtcacc accgagacgt cgtaatcgcc cggcgggatg ccgtaatccg cgggattcac
     9061 cgagaaatca acctggcctg gtccgacgga cgaactgcct tctgggatca gcgagacggt
     9121 gtagtcatcg cctgcggcat cggtaaagtg gagatcggcg gtggtacagg cgtcgtccag
     9181 cgtagcgcga ccgttaatgg tctggtcgct cacctgcaag gcggtggtct gcaccataat
     9241 gtcgtcgccc accaggttac cgagcgccat cacctgaatg ttgctcatca gcaccgcgtt
     9301 ggtgttggcc tgcacgctca tctcttccat catcgccacc tgcgccatcg acgacagctg
     9361 gtcgatgtat tcggtagcgt cggtcgggtc ggtcgggtcc tggttttgga tctcggcgac
     9421 aaacagcgtc aggaaggtat cgacaggcga gctatcgctg ctgctttgcg tgtcgctggc
     9481 cgccacatta ttgctttccg ccgtggcggt cggctgcggc gattgcgcat tcagcgccag
     9541 ggtattcatc aggcttctcc cagtttcagc aaactttgct gcatactttt cacgttgttg
     9601 agcacatcga cgttggtttc gaaatcgcgc gaggccgaca tcatgtccgc catctgctcc
     9661 accacgttga cgtcgggata ccagacgtca ccgttggcgt cggcgagcgg agaatgcggt
     9721 tcatagcgtt tcaccgcccc gccggtttgc agcacgtcct gtacctgcac gctggcaccg
     9781 tcaatggcat gacgatgtgt acccgccagc aggctgtggt gatacaccgc ggcaaacacc
     9841 gggctgcgcg ctttatacgc ctgcgcctcg ctgctggcgg gcgcattggc attcgccagg
     9901 ttactggcaa cggtattcag acgtagcgtt tgcgccgtca tcgccgagcc ggaaatctga
     9961 tagatatcag taaatgacat gcttatttcc cttcaatggc ctctctgatg ccgctgattt
    10021 gcagattcag aaaggtcaaa ctgctttgat aatccatact attttgtgaa aaccgcgcct
    10081 gctcgctgtt cagttccacg gtgttgccat cttccgacgg ctgcatcggc acgcggtact
    10141 ggacatccac cgccgcgtta tttgcccgtt gcatctccct ggcaaaatca atatctttag
    10201 ccttgaaatt tggcgtatcg acgttcgcca gattcgcagt cagtagctcg gtcctctcaa
    10261 gacgtaactt cactgcctgc ggatgtactc ccaatgcctg ctgaaaactg atccccattt
    10321 cctgcatcac tcctgcttca atccgcatac gctaagcaat cgccatgcca ttttttaagt
    10381 cactgatgag aaagggttta tgagttttca attttgcagg cggaagtttt acttcctcct
    10441 ggcgctggcc ttaccgggct acgccgccgt ccatcccgtg caacacagcg cgcgggaaca
    10501 agtcaacgcc caggtgctga atgccgcgag ccaaaaaatt gaatcgctgg cgcagcagcg
    10561 gcagtggcat gactatcgct acacctttaa gatctacatt ccctcgcaga ttgcgaccgc
    10621 cgcgccctgt actaaaacac ccggcgtcac gctgacttcg cctgcggaaa tcgccctcaa
    10681 ccggatgaac ttcaccgtta gttgcccgca aagctggcag atgaacgtgg cggtgcgccc
    10741 ggatgtactg gtgccggtgg taatgacgaa gtcactggtt gcccgcgata tgccgctcac
    10801 cgcgaatgat gtggagctta agccctacaa cgtcagcgcc cagcgccgcg atgtgttgat
    10861 ggagcttgat gacgcgattg gtttaagcag taagcacgca ttgcaaccag gcagacccat
    10921 caccaaagaa gaactggtgt ctccggtgct ggtcgggcgc gatcagccgg tgatgattgt
    10981 ttaccagagc gcggggatca ccgcctcgat gcccggcgtg gcgctgaaaa atggtcgtaa
    11041 gggcgagatg gtgaaagtgc gcaatgccag cagccagcgg gtcatcagcg cgatggtggc
    11101 ggaaagcggc gtggtgacaa cggtaagcgc ggagtaattt gcgcaggcga ttaaagtttt
    11161 gccgcctgtt aaccgcttta ccgcagtaac ccacccaaat accgagacga ggttgcgatg
    11221 aaaatcaccc cgactatgcc tggcaatcgc ccgacagcca caacggcagg ctccagccag
    11281 ccgcgcaaaa ccacggtgac caccaccacg acgctgagcg ccgacgacat tactcaggcg
    11341 ggattgcagt ctgcccagca aacgctgaac gaccagcaag agagcgatat cgacagcaac
    11401 aaagtggcgc aagtgcaggc gatgctggcg tccggcagcc cgcaagtgga cactgaacag
    11461 ttagccagcg acatgctgag ttttttccag aactaagagg ccgatgaagt gagtatccgg
    11521 ttacaacagg tgaaggcgtt gttgcagggc attcgtgacg atgacgcgct gtatgattcc
    11581 ctgcgcgaac ttctccagcg tcagcggatc tgcatgattc gccgcgccag cgaagagttg
    11641 ctggcggtaa acgatgagat aacccatcac tatgagcagt tgcatggaca tagccatcag
    11701 cggcatagcc tgctgaaaat gctcggtgtt agcgtaaatc gtgacggtct ggcgcaggtt
    11761 ttcacctggc ttccggctgt tcaaaaagcg gctgcacaac agttgtggca acgccttgag
    11821 caaaaagcgg aacgctgcaa agcctacaac gacaaaaacg gcgagctgtt gattcgccag
    11881 tatgaattta ttcagtcgtt tttaggcagc gaagcagatt ttctctatca ggaataacgc
    11941 gaataccttc cccatagcgg aaatggattt tccgcctgat ttccaatccc ttgtttttaa
    12001 ttacaaaaat ccccggcacg cttattgcgt atcctgcaca gcattaaaga ctggaagcca
    12061 cctatgagtt ttattgaatg ttacgaaccg gaatacgtgc gcaaattttt agcgcgtcac
    12121 cccggttcgc ctttatacgt ccgcaaggtg tggaaaaacg agattcgcct gagcctgtca
    12181 ttgaccgata ttgccagctg cgaagcggtg ctcaacgatg tcagggcctt tgatttacag
    12241 ctcacctacc gccctgtgga agataattcc gcagagcttt ccgcgcgcga tgcgatagta
    12301 aaccaggtga ttttgagtac cctcacgctc cgtgatttaa ccccggagct ttccctgtat
    12361 gctgtcggga ttttgctctc ctgcgccagc aaaatgccgg gcagagacgg agacatcctg
    12421 gcccgtttga cgacgctgcc acaggcgctg gccgatcatg cgaaaaaagg tacgctacag
    12481 gcacagtacg cccagctccc gcccgtgcca cagctggcgc gccagttgat gacgctgctc
    12541 ggcggttgtg cgtttgactg gtcgattttg ccggaaagcc cgcgcaaagc ctcgctgccg
    12601 ttgcaggtta ctttactgac gctgcatgac gccaacagcg aagccctgtt acaacagcag
    12661 cttcagacgc agtggcaaac cacctggcaa cagcattttg ccaccgcgcc ctggatgatg
    12721 cgtaactggc tgatttaccg cgtttaccac gatatgattg ggcagactga cggggcggat
    12781 tatttcccgc tggtgtgcga tttttatctc ctccgcacgt taatcagttt atggacgctg
    12841 gatggttctc cgctgcggca ggaggatatt ttcgccctct ttgccatgtt tgaacgctgg
    12901 cgagccagtg aaaatgcgct gttggttcgc cagcaaattc agtcgttatg tgccgccgac
    12961 ccgctgcttt cagcgttttc gctgctaacg tgagtcgctc tgcgcgcgct ttgcccctca
    13021 ccctaaccct ctccccaaag gggcgagggg accgatcgag cacggtgtta aaatcggcgc
    13081 tgattttctc cctctccctg tgggagaggg tcggggtgag ggcatcagcg cgcacgttca
    13141 tcacattcac acattaactc acccaaacac catgccaaca ccaaccatca tcaaaacact
    13201 cgccacccac cgcgaaaaac cgtttgtcag cgtcgtcacg ccaacctgga accgcggcgc
    13261 attcctgccg tacctgctct atatgtaccg ctatcaggat tacccggcag accgccgcga
    13321 actcatcatc ctcgacgatt ccccacaaag ccatcaacac atcatcgacc gcctgaccaa
    13381 tggcacgccg gaagccttta acatccgcta catccatcac ccggaaaaac tgccgctggg
    13441 caaaaaacgc aacatgctca acgaactggc gcgcggcgaa tacatcctct gcatggatga
    13501 tgacgactac tatccggcag ataaaatctc ttacaccatc gctatgatgc aaaaacaccg
    13561 ggcgctcatc tccggttccg accagatccc catctggtac agccatatca accgtatctt
    13621 tcaaagccgc agctttggcc cacataatat cctcaacggc accttttgct atcaccgtaa
    13681 ctacctgaaa aagcaccgtt acgacgatga ttgcaattta ggcgaagaga aaagtttcac
    13741 cgacaatttc agcgttaacc cgctgcaact gcccggcgag cgcacgattt tgtgtatctc
    13801 gcatagccac aacacctttg ataaagactt tattatcggg gccagcacgc cggtaaacac
    13861 ggcgcttacg gatatcgtcc gcgatccgct gctgcgcaat gcttatctga gtctgcataa
    13921 cgccactcat caccaggcga tcaaccatca ggcgatcgat caggtggtcg tcgtgaatct
    13981 ggataaacgc ccggatcgct tacaacagat ccgtgaagaa ctggcattgc tgcatattcc
    14041 acccgagaag atcacccgcc ttgccgccag tgaagatgaa aacggtcaac ggggccgccg
    14101 tcagtcgcac ttgcaggcgc tgcgtctggc gcaacagcac ggctggcaaa actatctgtt
    14161 actggaagat gacgcggtta ttctcaagca ggagaaacat attcaggtac tgaacacgct
    14221 gctggcgagc ctggcgaaaa ttccctggca ggtgatgatc ctcggcggcg aaatttcgca
    14281 aggaacgatg ctaaaaagtt tgcctgggct ggtgcacgcg cgggactgcc gcaaagtgtg
    14341 cgcttatctg gtgaacagcc gctactaccc gcaactggcg cagcagatga gcaacgatga
    14401 gcactcgctg gaagactgct ggcaaccgct gctacgcgcc gacaaatggc tggcctgtta
    14461 cccgagcctt tgttatcagc gaccgggctt tagcgatatc cagaaaaaaa cgactgataa
    14521 tattgctcgc tatttcaata agttacccgt aaccaccaag ccatccgcgt tacccatcgc
    14581 cgacaccatc ggctttttta tggaaacctc attccactac gcgctgtacc gcccaatcat
    14641 caccgcccta caggcgcaag ggctgagctg cacgctggtg atcaacgacc gcgtgtttaa
    14701 accgtttctg gatgagatgc tggaaacact gaaaaacatc gacgatccgc agcttaaagg
    14761 tatgcgtctg tcagaaatgc agacacatgg tcagcgggtg aaatgcctgg tcagccccta
    14821 ccacacgcct gcgctcaatg gcctggcggc ggtgaacatc cgcgctatgt acggcttagc
    14881 caaagagacg tggaaccacg ccgactggaa tcgtttttac caaagcattt tgtgttatag
    14941 ccactacagt cagcaggcgc ttgcgcattt cggcagcgca aaagtggtcg gcaacccgcg
    15001 cttcgacgcc tggcataacg gcacgttcga tcgcgcgttg ccggaaaaca tccaatcaga
    15061 ttaccgtaaa cctacggtgc tctatgcccc aacgtttggc gcattaagct ccctgcccca
    15121 ctgggctgaa aaattgggcc gcttgagcgg tgatgtgaac ctgatttgca aactgcacca
    15181 tggcacctgc tcacgcccgg aagaagcagc atcgctggcc ctggtgcgca ggcatttaaa
    15241 acagcgcacc gactccgccc gccacacgct ggcgctgctg gcaaaagccg attacgtgct
    15301 gaccgataac agcgggttta ttttcgatgc tatccacgtt gataagcggg tgatcctgct
    15361 cgactttccg ggcatgaatg atttgctcga cggtgagaaa agttattcca cggcagagag
    15421 cgccgaccag cgaatccgcg aaatattgcc ggttgctcat gatgttgcag aactgcgtta
    15481 tttgttgtca gaggcgtttg actgggggtc agtgcaggcg cggcttacgg agattcgcca
    15541 tcactattgc gatgcgttta tggatggcaa ggcgggagaa cgggcggcga ttgtgattgt
    15601 ggaggcgctg gggtaaaccg cgcaactgcc tgatgcgctt ctcttatcag gcctacggat
    15661 cgatcacgtc ctgtaggccg gataaggcgt ttacgccgca tccggcaaga aaatacatct
    15721 ctgttttagt actttataaa agccctctaa tcacctcaat aatccccgtc gtcgacaccg
    15781 acggcgtgcg ggggaaatag accacctcac aaatatacgc aaagctgtca aacttccctg
    15841 cccagtcatc ccccatcact aaaatatccg cactgtaccc gcgcaaatac tccgctttct
    15901 gctctagcga ttcttccaga aatacgccat ccacgcacgc cagcccggcg atgatcgcca
    15961 tgcggtcatc ctgatggtaa accggtgccc tgcccttttt ggcgatgttc agggcgtcgg
    16021 acgatacgcc gaccagcagc cgctcgccca atgcgcgggc gcgttgtaaa aggcgcagat
    16081 gaccgacatg aaacacatcg aaggtgccga aggtgatgac cgttttcatg gctactccgc
    16141 taatttgtgc cgcagccagc actgcacggc gatgtcgtcc accgcttttt gcgctttcgc
    16201 cgtggcgctt tcttgttgct cgcggcgttt gtcgtccagc gtcagggcga ctactttctc
    16261 ttcgcacgcc gccgccgtga ggtttttctg cattcgcgtg gctttgatat tggccagcgt
    16321 tttttcctgc tcctgccagg cgattacctt gcgcagggtg cctttgtagc cgctgacgtt
    16381 tttcagcgat tccaccgaca ccgccgcacc cgcagatgtt ttctcgacaa ggtagcctaa
    16441 cgccttgata ttgttgtcat aacccgcgca aatctgtttt tgctttgcca gctcgacggt
    16501 tttgtctttg accgatttat cccgcaggcg ctgcaactgc gtcagggtgt cgatgatttg
    16561 tcgcatagtt actgttctgc ctttttgaca ccaggaaaga gaatttgcag gcggctttgc
    16621 accagttcga gggaggcggg ttcgcgcatc tcctggcgca aaaatcgctc gatggccggg
    16681 aacattttta ccgctttgtc gacgctggcg tcggcacctg ccacataacc gccgagcggg
    16741 atcagaggtt tgatctccat ataggcggca tagttttgct ttaacgcgcg ggcggattgc
    16801 tgatgttcca ggctggtgac ctggttcatg cagcggctga tcgactggcc gatatcaatg
    16861 gccggatagt gccccgcttc cgccagtttg cgggttaata cgatatgccc gtccagcacc
    16921 gcgcgcgcgc agtcgacaat cggatcttgc tggtcgtcac cttccgccag cacggtgtag
    16981 atggcggtca tcgatcccgc gctttcgctg ttgcctgcgc tttcgaccag tttggggatc
    17041 atgccgaatg ccgatggcgg ataacctttg gtggcgggcg gttcgccgag cgagagggcg
    17101 atttcccgct gcgccatcgc gtagcgggtc agggaatcca ccagcagtaa gacgtgatgc
    17161 ccgcggtcgc gaaaccaggc ggcgatggag tggcacagtt cggtggcttt cagacgcatc
    17221 agcggcgatt catcggcagg cgcgacgacg acgatagact ttgccagccc gtccgcgccc
    17281 agcgagtggt cgataaactc tttcacttcg cgcccgcgtt ccccaatcag cccaaccacc
    17341 acgatatcgg ctttggtctg gcgggtgatc atccccagca gcacgctttt cccgacgccg
    17401 ctgcccgcca ttagtccgac gcgctggcct ttgccgatgg tcagcaggcc gttgatcgct
    17461 ttcacgccca cgtccagcgg ttcgtctacc gaacgacgag ttaagggatt aaccgagggc
    17521 ggcaacggcg gcagaaggtc gttgccgttc aggcggcctt tgccatccag cggttcgccg
    17581 aggccattca ccacgcggcc taaccagctt tcgcctatca ggatgtcgtg ggctttttct
    17641 tccgggaaga cgcgtgcgcc tgccatcagg ccgaccggct gtttaaacgg cattaaatag
    17701 gtgatgtcgc gattaaagcc cacggcctgc gcgtcgatca gcgtgtgatt ggcactttcg
    17761 atgcggcaca gctgcccggt cattagcggg cagccgacac tctccagcag gatgccgtta
    17821 aggcgcacca gccgcccggc gacgcgggcc agcgggatcg actccagcga gcgcagggcg
    17881 ttatcgaacg cagaaaaatc actcaccctg cgactccggc gtgagtgttt ctttcagcgc
    17941 ggtcatgcat tgttcaaggc gatgctcgca gccgatatcc agttcggatt tgtcggtgat
    18001 cacccggcat tcgcccggtg gcaaatccgg cgaaggcgtc agcccccatt cgctgacctt
    18061 ttccggcacc gcgtcgcgca ggcggttaaa ctcttccgtg ctcagcatta cttgcagggt
    18121 ttccggcatc gccgggaagg ccgccagcgc ctcttccacc agcgccagca actgcgtggg
    18181 ttgcagcgcc agttcacaac ggatcacctg gcgggtgact ttttccacca gttgcagcaa
    18241 atcttcccgc tgtttacgtt cgatatgagc gaggaagtcg ttcactttac cggtgatggc
    18301 ttccagcggc tgcgccgcct caacaaactg tttgcgccct tcctgctgcc cggcgaggct
    18361 gccttcggtg taaccctggc gtcgcccctc ggcaaaacct tgctgatgac cttcgctaaa
    18421 gccctgctcc tggccttcgg tcattccttg agcaaaccct ttttgcagcc cttcctgaaa
    18481 tccgtccatc aactggcgct ggtaatcggc ggcgctaata ctggtagtga gcggttctcc
    18541 ggtcaccgca cgctggcgcg gcgggaaacg gtgcaggcga tagcgcccgc gcagtgtttc
    18601 aatggtcatg gttactccgc cgtctgttcc gcaaagagct gtagctgaat ttcgcccgcc
    18661 tcttccagtt cacgggcgat gcccatgatt tcgcggcgga tctgttcgat acggctgacc
    18721 ggcaccggcc ccagacgcgc ggtgatggct tccagttgct gcgcctgccg ttttggcatt
    18781 accgcgtaaa tcgaacggcg cagcagggct tcggtgcctt tcagcgccac cgcccagtct
    18841 tccatcggca cttcgtcaag cagacgtcga cgcacctctt cgttctggcg gctgaggata
    18901 aagaagtcgt acatctcatc ctgaagctgc tccagcacct cttcgtcgcg ctcgcgcatc
    18961 tggtcgagga tcacctgctg gttgccctgg aagcggttga cgatatccgc cgcctgtttg
    19021 atgcctttca cttttgagcc gtgttccgaa agcaccgaca ggccgcgttc gatcagcctg
    19081 tccagttcat ccaccacgtc gcggttgacg tcgttgagtt tcgccacccg gtagagaatt
    19141 tcgttttgca ccgattcgtt gagatacgag agcaccgctg ccgagatttc cggcgtcaga
    19201 aacgcgagga acaccgcctg taattgcaga tgttcttcgg agatcagcat cgccagctgg
    19261 cgtggttcca cccattgcag gcgcgccatc cgcgagcgga tctcatcgcc gtagatgccg
    19321 ttgatcacac tgctggcgat ctcggtgccg agggctttgt tgagaatacc ctgcaacatg
    19381 gatcgcgatg cgccgttgat cccgctctgt tcgcggaact catcaaagaa gttattgatc
    19441 actttgcgcg ccatgctggt tttcacgccg gaaagccgtg ccatgttttc gctcaggcgc
    19501 cccacttctt cgcggctgag tttttgcatc accgtcgccg ccgcctcttc gcccagacac
    19561 agtagcagga tcgccgcctg ttccaggtag ctgttgctct tatttgttgt caattcgctc
    19621 attgctggtg atccattgtc tgagaacttc ggctacgcga tcggtgtcgc tcatggccag
    19681 tttttgcagg aactcgagtt ttacttccag gccggaactc tgagacggca ggctgtcatc
    19741 gccgggcagc gacggcagat cgacgttttt gcgctcctct tccggcatgg caatccacgg
    19801 ttcgctgacg gcttccgatt ctggtgtgat aatcggtgca cgtaccgccg tcagccgttt
    19861 cattaccggg cgcaccacga agaacagcaa caacagcgcc agcaggccgc agccaatcag
    19921 gcgcacccag gccagcacgc tgtcatcttt ccacagcgga ataattggtt cgaccggcac
    19981 cgcctgcggc acaaagttaa gcagtgaaag cgtcaggtta tccccacgtt gcgcgtcaat
    20041 accggcggca ttgttcagca acgcggtaag ctgcgcggtc tgctccggct tccagttttt
    20101 cagcgccgga gcgttttgat tgagcaccac cgccacattg aggcgtttca ccgcaaagcc
    20161 gggatgctga atatgctgga cgctacggtc gtaagagtaa tcgcgcttgc tttcgctgtg
    20221 tttggataac gcttcaggcg agcgattttc ttccgtaccg ttagtcattt gattcgccgc
    20281 aaccggcgga cggttgctga gagagccggg cacacccatc gccacctgat tggtgtcgct
    20341 gtccagcacg ctctcttcgc ggtttatttt cggcgcgtcg ccgtagtgtt cctgagtttc
    20401 gtcgatggtg ctgaggtcga gatccggcat cacgctgaca cggtaattcc cgctgccgac
    20461 cagcgaatcc agcacgttcg ccacgctggc gtgggttttg tcctgaatgt ctttgaggat
    20521 ctgatcgcgt ttacgggtag cggcagagac cgcctcgccc gcgccgatgc cgtcggtcag
    20581 cagatttccc gcctgatcaa ccacgctaac tttgctggcg tgcagccccg gaatgctgcc
    20641 ggaaaccaga tgcacaatgg cgttcacctg gtccatattc agcttcgcgc cgtagtgcag
    20701 acgcaccacc accgaggcgc tgttttgcgg ctcatcgctg accacaaacg agctttcttc
    20761 gttgagcgcc agatgcaccc gcgcgctctc caccgcgtcc agcgacataa tgctctgcgc
    20821 cagctcccct tcgaggctgc gtttatagcg cacgttctgc acgaactggc tgctgccaag
    20881 cacttcgtct ttgtccatca gctcgtagcc gctgggcaaa atcgcctgca cgcctttggc
    20941 ggcaagggtc atgcgcgttt tcgacagcgc ctcttccggc accagtatct gcccgctttg
    21001 cgggtcaatg cggtaggtga gcttttcgcc gtccagcacg gtgacaatct gcgacacggg
    21061 cagattttcc tggcgtccgt agagcgagac gtagccgcga ttgccggtcc acagcacgct
    21121 aacgataatc gccgtcgccg ccacggcaag ccccacaccc gccatcagcg cccagcgttt
    21181 gttatctgcg agacgcagtc gaaacgccgg aaacgccagg gttaacttct ttatttgtgc
    21241 attcataggg ttagatcgac atactcatca gatcgttaaa tccggtggcg attttgttgc
    21301 gcacctgcac cagcgcggag aaagagagcg acgcctgctg gctggcgatc atcgcacctg
    21361 ccagatcatc gcttttgccc atgtcgatag ccgtttgctg ctgctccgcc acctgctgga
    21421 actggtcaac gtgaccgagc gcgccctgca taatgcggtg aaacgaaacg tccgggtcgg
    21481 tggaactgaa ggtcatggcg ggcagcaccg gcgcctgggc gttggcctgc atccgctgca
    21541 cgtcctgcat gatctgcgcc tgcatggtgc tgttgattgt ggtaatgctc atttttaact
    21601 tccgggtcag gagaaaactt cgataccgtg cttgcgcatg gaggccagtc gatagcgcag
    21661 tgcgcgcggg gtaataccta acaggtcggc gattttgctg cggttgccct gatatttacg
    21721 catcaggtca gcgatatatt gatactgggc gcttcgtccg tgctggccta aatcgctggt
    21781 ttcggcaatg tgtacggcgg gctgagcagg ccattgcagt tcgctgtggt cggcaatgtc
    21841 attttccggc aggcctaagg cgtccgggta aatcacgccg tcgcgattta aaatcattcc
    21901 ccgctgaatg gcgttctcca gctggcgcac attgccgggc cagcggtaat tcagcatcgc
    21961 acggcgggta gattctgaaa gggtgatatt tttcaccagc accgtggagt atttttttat
    22021 aaatgactct gccagcggaa taatatcgtt cagacgttca cgcagcggcg gcatggtaat
    22081 aggaataacc gccagacgat aatagagatc ttcacgaaaa cgcccggcgg cgacttcctg
    22141 ctcaaggttt ttattggtgc aggcaatcag gcgaaaattg agtttaattt gtcggttgct
    22201 gcctaatcgc tcaacctgtt gttcctgcaa tacgcgtaat attttggcct gtaacgccag
    22261 cggcatatcg ccaatttcat cgagtaataa ggtgccgtta ttcgccagtt ccatttttcc
    22321 tggcacactg gcaattgcgc cggtaaatgc gcctttgtcg tagccaaata aggttgcttc
    22381 cagcatgttt tccgggatcg cagcgcagtt cacgccgata tagggcgcgt tttcattttc
    22441 accaaaggcg actgtgtgaa tatatttcgc cacgcactct ttgcccgcgc cggtttcgcc
    22501 ctgaatgagc accggcacgt taaatgcggc gacacgcttt gccagagtaa aagcgttaat
    22561 gctggaagcg gcagttgcaa taagttcaga catagggtga acgatataag tagcgacaat
    22621 aatcaggatg ttctgaagcg tatcaccggc gggattaaca agttcgaatt aaacgataat
    22681 aaaaacatta tttgtttata aacaatgatt tatccatgta ggccggataa ggcgtttacg
    22741 ccgcatccgg caattgtgca ccggtgcctg atgcgatgcg gcgcgtctta tcaggcctac
    22801 ggttcgtgca atgtgttatg acgatgcctg atatctcgcc ctttaataat ttaattattt
    22861 tttcacccta ttaataattt gcccctttca cgccccctct atttaatacc tcaccttgtg
    22921 tcactgccgg atttgcgtgc acaaaatcgc ccataccatt acagcagaga aagtaagcca
    22981 tgctgaagta cagtaaaacg ccaggcatct ttaaattaga aggtaatcgg ctcggacgcc
    23041 cctaccatcg cctgccgacc ctgtttacag ggaattttga cgtgattgat tcgcatctgg
    23101 gcagctactt tctcaaaaag catcgcagca atatcaccct aaaaaagatt ggctgcgaaa
    23161 tggatattat taataaaaac gctgaactga tggtttccca ggtcgggcat ctggcgtttg
    23221 atattgatcg ctctttgctg ttaatgctgc tgggcaattt ttatggtctg gagtcctctc
    23281 ttgaagaggc gaaagcgcat aacgggctgc cgaccaaaac cgaaacgcgg ttaaaaaacc
    23341 gtctggcgct ggatatctgc acgctgattt ttaatctgca aacctccggt attgcgctga
    23401 aattaaagct ggacagcagc acggtaatta cccactgggc gtatcaatta acctttaccc
    23461 ttgcgggcga tgaagagagc tgttttcgta ttttgctcga cgacgcccac accgatttta
    23521 ttttaaatct gatccgccac agcgagcaca gtaaaccgca gcaggtggcg aaatcggtca
    23581 ataaaccggc cctgattaaa gagatcatcc gctccctgcc gctgaccctg aacgtcaaaa
    23641 tcgccgagct atcgatgaat gtcgcggatc tcacgcagat aaaagccggg gacattttac
    23701 ccatctcgct gggagaaacg ttcccggtgg cgattgggca atctgaatta tttagcgccc
    23761 tgattgtgga agacaaagac aaactgtttt tgtcggagct ggcgggcaaa aatgagaata
    23821 gccatgagta agcaagaaga tattttagcc ggggattttg gcctcaccga tgaagttgcg
    23881 cctgtcgcaa aatccgccga cgctgaaacg ctggtcaccc gcctggaaga ccgtttttcc
    23941 gactcgatga cgctgctaaa acgcattccg gtgaccttaa cgctggaagt gtcatcagta
    24001 gaaataatgt tagccgacct ccttaacatc gacgacgaca cggtgattga gctggataaa
    24061 ctcgccgggg agccgctgga tatcaaagtg aacaatatcc tgctgggcaa agcggaagtg
    24121 gtggtggtca acgagaagta cggcctgcgc gtgctggaat ttaacacccg cgatatcaac
    24181 gacctggcac catgaaacgc cgcactcaac tgacgctggg ccttgggctg ctggcgcttg
    24241 cgccggtggc gttggcccag ggcggcgata ttgcgctgct taacgtggtc acccacggca
    24301 acactcagga gtacagcgtc aaaatccagg tgctgattct gatgaccctc gtcggtttgc
    24361 tgcctacgat ggtgctgatg atgacctgct ttacgcgctt tattatcgtg ctgtcgctgc
    24421 tgcgccaggc gctagggcta caacaaacgc cgccaaaccg cattctgatt ggcattgcgc
    24481 tctccctcac catgctggtg atgcgcccca tctggctcaa tatttacgac cacgcagtag
    24541 tgccgtttga aaatgatcag attaccctga ccgatgcgtt aagcaccgcc gcgacgccac
    24601 tcaaacgctt tatgctggcg cagactgaca aaaaagcgat ggcgcaaatc atgactatcg
    24661 gcggcgcgaa gggccatgcc gccgaccagg atctctcgat tgtggtgccc gcctatgtgt
    24721 tgagcgagct gaaaaccgcc ttccagattg gctttatgat ttacatcccg tttctggtta
    24781 tcgacctgat tgtcgccagc gtactgatgg cgatggggat gatgatgctg tcgccgctga
    24841 tcgtttcgct accctttaag ctgatgctgt ttgtgctgat cgacggctgg tcgctgacca
    24901 tcggtacact caccaccagc attcgcgggc tggggctggg ctgatgatgc tgaccataga
    24961 cgtagccgcc gacattgtcg ccagcggcat aaaagtggtg attttgctgg tttcggtgct
    25021 ggtagtgccg agcctgctgg tcggcctgct ggtgagcgtg ttccaggcgg taacgcagat
    25081 taacgaacag accctaagct ttttgccgag gctgattgtc acgctggtgg tgctgggcgt
    25141 gtgcggtaaa tggatgatta tccagttgca tgatctctgc attcacctgt ttacccaggc
    25201 cgcgttgctg gttcattgag atgcgcacaa gcgacgttac gcaactgatg gatctggcgc
    25261 tggggctgtg gtttcccttt gtgcgcatta tggcgtttct gcgctatgtg cctgtgctgg
    25321 ataacagcgc cctgaccgtg cgcgtgcgga ttattttgtc gctggcgctg gcaatcatca
    25381 tcacgccgtt gatcccccat cctgttcccc acgacctgat atcgctcaac agcctgatcc
    25441 tgacggtgga gcaaatttta tggggcatgt tattcgggct gatgtttcag tttttgtttc
    25501 tggcgctaca gctggccggg caaattctct cgttcaatat ggggatgagc atggcggtga
    25561 tgaacgaccc gagcagtggc gcatcgacca ccgtgctggc ggagctcatc aacgtctacg
    25621 cgatcctgct gttttttgcc atggacgggc atctgctgct ggtgagtgtg ctgtataagg
    25681 gctttaccta ctggccgatt ggcaacgcgc tgcatccgca aaccctgcgc accatcgcgc
    25741 tggcctttag ctgggtgctg gcgtccgcat cgctgctggc gctgccaacc acttttatca
    25801 tgctgattgt gcagggctgc tttggcctgc tcaaccgcat tgcgccgccg ctgaaccttt
    25861 tttcactcgg ttttcccatc aacatgctgg cggggctggt ctgctttgcc accctgctct
    25921 acaacctgcc ggatcactat ctgcatctgg cgaattttgt gttgcagcaa ctcgacgcgc
    25981 tgaaaggtca ctatggcgga tagcagcagc gaagaaaaaa cagagaaacc ctcggcgcaa
    26041 aagctgcgta aagcgcgcga agaggggcaa ctgccgcgct cgaaggatat ggggctggcg
    26101 gcgagcctgt ttgcggcatt tgtggtgatc tccagcagct tcccgtggta tgccgatttt
    26161 gtgcgcgaga gttttatcag cgtgcaccag tatgcacagg agattaataa cccagaggtg
    26221 atcggccagt ttctgcgcca tcacctgtta attctcggca agtttattct caccttgctg
    26281 ccgatgcccg ccgccgcgct actctcctca ctagtgcccg gcggctggct gtttctgcct
    26341 aaaaaaatcc tcccggattt cagcaaaatc agcccgttaa aaggcattga gcggctgttt
    26401 tccagtgaac atctggcgga aacgggaaag atgacggtga agtcggtggt ggtgctggtg
    26461 atgctgtggg taagcctgcg caataacttt gcctcctttc tcggcctgca ggcgctaccg
    26521 ttcaaactgg cgatgaatga gggtttatcg ctctacgcct gcgtgatgcg caactttgtc
    26581 atcctgttta tcttctttgc gttaatcgac gtgccgctgg cgaaagccct gtttaccaaa
    26641 gggctgaaga tgaccaaaca ggagctgaaa gaagagtata aaaaccagga aggcaagccg
    26701 gaagtgaagg cgcgcgtgcg gcgtctgcaa cggcagttgg cgatggggca aattcgcaaa
    26761 gtggtgccga aagccaacgt ggtgatcacc aacccgaccc actacgccgt ggcgttgcag
    26821 tatgactcgt cgcgcgccgc cgcgccgttt gtggtggcaa aaggcaccga tgaaatcgcc
    26881 ctctatattc gccaggtcgc cgctgagaat caggtggaag tggtcgagtt cccgcgactg
    26941 gcgcgctcgg tttactacac cacccaggtg aaccagcaaa ttccgtttca gctttaccgg
    27001 gccatcgccc acgttctgac ctacgtcctg caaatgaaac actggcgcga gggtgcacag
    27061 ccgcgcccgg ccctgaacag acatatttcc attcccaaag aggtgcttaa actggatggc
    27121 gaaaacaact aaatcgttcc tcgcgctgct gcgcggcggc aatcttggcg tgccgctggt
    27181 gatcctgtgt attctggcga tggttatttt gccgctgccg cctgcgctgc tggatattct
    27241 gttcaccttc aacattgtgc tggcggtgat ggtgctgctg gtggcggtgt cggcgaaacg
    27301 accgctggaa ttcagcctgt tcccgaccat tttgctgatc accaccttaa tgcgcctgac
    27361 gctcaacgtc gcctctacgc gcgtggtgct gctgcacggg catctcggcg cggacgcggc
    27421 gggtaaggtg attgagtcgt ttggtcaggt ggtgatcggc ggcaactttg ttgtcggctt
    27481 tgtggtgttt atcatcctga tgatcatcaa ctttattgtc gtcaccaaag gggccgagcg
    27541 tatttccgag gtttcagccc gctttaccct ggatgcgatg cccggtaagc agatggcgat
    27601 tgacgccgat cttaatgccg ggttgatcaa tcaggcgcag gcacaaaccc gacgtaaaga
    27661 tgttgccagc gaggccgatt tctacggcgc gatggacggg gcatcgaaat ttgtgcgcgg
    27721 ggatgctatc gccgggatga tgattctggc gatcaacctg atcggcggcg tctgtatcgg
    27781 gatcttcaaa tacaacctga gcgccgacgc cgccttccag cagtatgtgc tgatgaccat
    27841 cggcgacggg ctggtggcgc aaatcccttc cctgctactc tccaccgcgg cggcgattat
    27901 cgtcacccgc gtcagcgaca acggcgatat cgcccatgac gtgcgtcacc agttgctggc
    27961 aagcccatcg gtgctctaca ccgctaccgg aattatgttc gtactggcgg tagtgcctgg
    28021 aatgccacat ctgccgtttt tgctgttcag cgccctgctt ggctttaccg gctggcggat
    28081 gagcaaacgc ccgcaggcgg cggaagcgga agagaaaagt ctcgaaacgc tgacccgcac
    28141 catcaccgaa accagcgagc aacaggtcag ttgggaaacc attccgctga tcgagcccat
    28201 cagcttaagc ctcggctaca agctggtggc gctggtggac aaagcccagg gcaacccgct
    28261 cacccagcgt attcgcggcg tgcgacaggt gatatccgac ggcaacggag tgctgctgcc
    28321 ggagatccgc attcgggaaa acttccgcct taagcccagc cagtacgcta ttttcatcaa
    28381 cggcattaag gctgatgaag cggatattcc ggcggataaa ctgatggcgc tgccctccag
    28441 cgaaacctac ggcgagatag acggcgtgct ggggaacgac ccggcgtacg ggatgccggt
    28501 gacctggatc cagcctgcgc agaaagcgaa ggcgctgaat atggggtatc aggtgatcga
    28561 cagcgccagc gtgattgcta cccatgtaaa taagattgtg cgcagctata ttcctgattt
    28621 atttaactat gacgatatta cgcagttgca taaccgtttg gcgtcgatgg caccgcgcct
    28681 ggcggaggat ttaagcgcgg tgctcaatta cagccagttg ctgaaagtgt accgggcgct
    28741 gctgaccgaa ggcgtttccc tgcgcgatat cgtcaccatc gccaccgtgc tggtcgccag
    28801 tagcgcggtg accaaagatc atattctgct ggcagccgat gtgcgcctgg cgctgcggcg
    28861 cagcattacc catccgttcg ttcgcaagca ggagctgacg gtgtataccc tgaataatga
    28921 gctggaaaat ctgctgacta acctagtgaa tcaggcgcaa cagggcggta aagtgatgct
    28981 cgacagcgtg ccagtggacc cgaatatgct caaccagttc cagagtacga tgccacaggt
    29041 gaaagagcag atgaaagcgg cggggaaaga cccggtgctg ctggtgccac cgcagctgcg
    29101 ccctttgctg gcgcgttatg caaggttgtt tgcgccgggg ctgcatgtgc tgtcgtataa
    29161 cgaagtgccg gatgagctgg agttgaagat tatgggggcg ttgagttgat gggggattct
    29221 tttccatgct ttgcagcacc tgacaccatt aattgacgtc acactgcaaa gagactacga
    29281 tgacaccata caatgacgtc atggattgat agtatgatta aggtcagcgc gctaggttct
    29341 cgtcatagaa aaacattcga cgatgtaatg acggagccgc caaaatctgg aattaaatgg
    29401 gatgatgttg ttgcactcat taaagctgtg ggtggaacaa taaaaaataa caacggctcc
    29461 agacgaaaat ttcagattgg aacgaccaaa ttcagcacac atgagccaca tccaaaaaat
    29521 gtgatggata aaggcgcagt agcaggtctt aaggagtggt ttgttaactg tgtaggggta
    29581 gattatgaat aaagcatcga acattatcac aattaaaggt caaccagccg cagtaacatt
    29641 tgaagccgat atcaacgcat ttcgcggcaa gttcctcaat gtaaacggtt attgtgactt
    29701 tgtagccaca agtattgagg cattatatcg tgaaggcgaa agcgcgctgg atgagtggat
    29761 ggcggattgt gaagaggacg gtattgcccc ttttcgtgaa gaagaagagc aaaaacgcct
    29821 gacgcttcgt gttcctcatc acattgattc gcggctgacc atcgtcgcca gacaacattc
    29881 catttcaaaa aaccagctca ttgttgatgt cttagaacgt gagttttcag cccagatgta
    29941 aacaggtcgg atgccgcgtg gcatccgacc ttttgccagt tcgtcaatat tacccattta
    30001 caaaatcgtc tcctccgtca aaatccgccg cgcgcctaaa aagtgatcct gccagaaagg
    30061 ttcagctaac tgacttaccc gaatggtttc cccggtgcgc ggtgactcaa taaactgacc
    30121 gtcgcccaga tacaccccca tatggtcggc aatctcgcgg ctgtggatat ggaaaaatag
    30181 caaatctccc cggcgcaggt cgttattcgc cacaatggtt gcccggcgat agtgatacat
    30241 ctcattagcc gtgcgtggga gtttcgcctc aaggatcttg ttgtaggcat aaaaaactaa
    30301 cccgctacag tcgaagcctt gatcgggccg cgtaccgccc cagacgtaag gcttgccaag
    30361 ctgttgctcc agacggtgaa tcgccacttc ggtgatgtta tgcagatggt cgctgcttaa
    30421 gaaatggttg ttttccgcca gcgcctgcca gcgtctgtca ctcgccttga gcggcgcagg
    30481 aaaccattct ggatgctgtt ttatctgttc gcggttgtgc tggcgtagcg cccttctgct
    30541 ttcggcattg ccttccacaa tatagctggc ctgttttttc agatgggttt ggtactgttt
    30601 taataaacgc gcacgatttt gcatccgctg gcgggcggcg tagctgaaag tgatatgtga
    30661 tgtggtggcg ctggcaaatg aagaaaacgg caagcaaagc aacagtaatc ccgacaacac
    30721 aaaagaggga atcgacggca aggacatact caataatggc tgatcatgca ttgatgttgc
    30781 agtgtatccc ggcccgtacc tgaacaaact taataaacca ttattataaa attaataaat
    30841 atatttcagt taattatgta aaaacatccg tttattaaat tactcatgga ttgtcgctgt
    30901 tgctcatttg agctacaatt caagctgaat aaatatacag cacaggagat accccaatgg
    30961 ctgctaacgc atttgttcgc gcccgaatcg atgaagatct gaagaatcag gcggcggacg
    31021 tactggccgg gatggggctg accatctctg acctggttcg cataaccctc acaaaggtcg
    31081 cgcgtgaaaa ggcattgccg tttgatttac gcgagcctaa tcaattaacc attcaatcaa
    31141 tcaaaaacag cgaagctggc gttgatgttc ataaggccaa agacgccgat gatttatttg
    31201 ataaattagg aatttaaatc aaagggatat tgaatactcg ggacaatttt caaaggatgt
    31261 aaaacttgca caaaagcgtc ataaggatat gaataaattg aaatatctta tgacgcttct
    31321 tatcaataat gctttaccgc ttccagctgt ttataaagac cacccgctgc aaggttcatg
    31381 gaaaggttat cgcgatgctc atgtcgaacc ggactggatc ctgatttaca aacttaccga
    31441 taaactttta cgatttgaga gaactggaac tcacgcggcg ctctttgggt aaagcacgct
    31501 gccgatgaaa tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt tggtgataat
    31561 gtgagccttc caaatccaga ctattccgat ttgggatcta ttttgccgtc ctggcgtgtg
    31621 taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg cattaattct tgcgatgttg
    31681 ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac cacgccagtg
    31741 agcaaagagt ataagcagca gttgatggga tcccctgtct acatccagat cttcaaggaa
    31801 gaacgtacgc tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct cgacagttat
    31861 aaaatctgta aatattccgg cggcttaggg ccaaaacagc gtcagggcga tttcaaaagc
    31921 ccggaagggt tttatagcgt ccagcgtaat cagttaaaac cagacagccg ttactacaaa
    31981 gcgattaata ttggtttccc caatgcctat gaccgtgcgc atggttatga agggaaatac
    32041 ctgatgatcc acggcgattg tgtttccatc ggctgctacg caatgaccaa tcagggtatt
    32101 gatgagatct tccagttcgt tactggtgcg ctggtgtttg gtcagccgag cgtgcaagtg
    32161 agtatttacc cgttccgcat gaccgacgcc aatatgaagc gccataaata ttccaacttt
    32221 aaggacttct gggagcagct gaagccgggc tacgactact ttgagcaaac ccgtaagcca
    32281 ccgaccgttt ctgtggtcaa tggccgttac gtggtcagca agccgttaag ccacgaagtt
    32341 gtgcagccac aactggcatc aaactacacg ctccccgagg caaaataagc gccattcgcc
    32401 tggcataatc ttttgccagg tttcattgcc cgtcagcggc tgtgttgcaa tcaccgtgac
    32461 cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat ccagcagcgt
    32521 tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc aatacgccat
    32581 cacatagcgc ccgtcagaaa gcagcatgtt gaaaacgccc ttctgccgca gttcatccgc
    32641 cagtgaggcg atatatttaa acactgccgc catgttgccc ggtgtgcgcg ggtaacgctg
    32701 cgttaattta tgcaggagcc agcagaaggc tttttcgcta tcggtttcac cgaccgggcg
    32761 gaagttgccg gtttccagtg atttgtagcc cgtcagttgt ccgttatggg cataagtcca
    32821 gttacgcccc cataactcgc gggtaaatgg gtgagtattt tccagcgcca cctcgccccg
    32881 attagcctgg cgaatatgag ccaccaccga acaggatttt atcgggtagt cctggacaag
    32941 tttggcgatg ggggaattaa agctgggttg tggatcttta aatgtgcgac agcctttacc
    33001 ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg gttccaccac cacgctgtac
    33061 aagtccggtg aaactaaagc agatatcggt agggacgttg gcgctcatcc cgagcaattc
    33121 gcacatgggt aacctccaca acagcgggag cgaatggccc ccggcgaaga ttgatttaag
    33181 aattttcagg tcggatgcgg cgtaaacgaa ttattcggcc tacggttcgg gtacagattt
    33241 gtaggcatga taagacgcgt cagcatcgca tcaggcatca gcgcacaagt gccggatgcg
    33301 gcgtaaacgc cttatccagc ctacgccaga cttacttaac catctctttt tcaattaact
    33361 ggatcaggat atggatcact ttaatgtgaa tctcctgaat gcggtcggca taaccaaagt
    33421 gcggtacgcg aatttcgata tccgccgtgc cagccatttt gccgccgtct ttaccggtca
    33481 gggtgatcac tttcatcccc ttctcacgcg ccgctgcgat cgctttgatc acgtttgcag
    33541 agttaccgga ggtggagatc cccagcagta catcgccttc gcgacctacc gcttcaacgt
    33601 agcgggagaa aatatcattg aaaccgaaat cattaccgac gcaggaaata tgactaacgt
    33661 cagaaatagc aatcgccggg tagcccggac ggttttcacg gtagcgaccg gtcaactctt
    33721 cggcaaagtg catagcgtcg caatgggaac cgccgttgcc gcaggaaagc actttgccac
    33781 cggctttaaa gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga atattggcgt
    33841 catcttttaa aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta cgaataagat
    33901 cctggtacat gaggatatcc ttcagcataa atgtaataga caaaatgcag tgtaccggat
    33961 accgccaaaa gcgagaagta cgggcaggtg ctatgaccag gactttttga cctgaagtgc
    34021 ggataaaaac agcaacaatg tgagctttgt tgtaattata ttgtaaacac attgctaaat
    34081 gtttttacat ccactacaac catatcatca caagtggtca gacctcctac aagtaagggg
    34141 cttttcgtta tgatgatttt gagtattctc gctacggttg tcctgctcgg cgcgttgttc
    34201 tatcaccgcg tgagcttatt tatcagcagt ctgattttgc tcgcctggac agcctccctc
    34261 ggcgttgctg gtctgtggtc ggcgtgggta ctggtgcctc tggccattat cctcgtgcca
    34321 tttaactttg cgcctatgcg taagtcgatg atttccgcgc cggtatttcg cggtttccgt
    34381 aaggtgatgc cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg caccacctgg
    34441 tgggagggcg acttgttcca gggcaagccg gactggaaaa agctgcataa ctatccgcag
    34501 ccgcgcctga ccgccgaaga gcaagcgttt ctcgacggcc cggtagaaga agcctgccgg
    34561 atggcgaatg atttccagat cacccatgag ctggcggatc tgccgccgga gttgtgggcg
    34621 taccttaaag agcatcgttt cttcgcgatg atcatcaaaa aagagtacgg cgggctggag
    34681 ttctcggctt atgcccagtc tcgcgtgctg caaaaactct ccggcgtgag cgggatcctg
    34741 gcgattaccg tcggcgtgcc aaactcatta ggcccgggcg aactgttgca acattacggc
    34801 actgacgagc agaaagatca ctatctgccg cgtctggcgc gtggtcagga gatcccctgc
    34861 tttgcactga ccagcccgga agcgggttcc gatgcgggcg cgattccgga caccgggatt
    34921 gtctgcatgg gcgaatggca gggccagcag gtgctgggga tgcgtctgac ctggaacaaa
    34981 cgctacatta cgctggcacc gattgcgact gtgcttgggc tggcgtttaa actctccgac
    35041 ccggaaaagc tgctgggtgg cgcagaagat ttaggtatta cctgtgcgct gatcccgacc
    35101 accacgccgg gcgtggaaat tggtcgtcgc cacttcccgc tgaacgtacc gttccagaac
    35161 ggaccgacgc gcggtaaaga tgtcttcgta ccgatcgatt acatcatcgg tgggccgaaa
    35221 atggccgggc aaggctggcg gatgctggtg gagtgcctct cggtaggccg cggcatcacc
    35281 ctgccttcca actcaaccgg cggcgtgaaa tcggtagcgc tggcaacggg cgcgtatgct
    35341 cacattcgcc gtcagttcaa aatctctatc ggtaagatgg aagggattga agagccgctg
    35401 gcgcgtattg ccggtaatgc ctacgtgatg gatgctgcgg catcgctgat tacctacggc
    35461 attatgctcg gcgaaaaacc tgccgtgctg tcggctatcg ttaagtatca ctgtacccac
    35521 cgcgggcagc agtcgattat tgatgcgatg gatatcactg gtggcaaagg cattatgctc
    35581 gggcagagca acttcctggc gcgtgcttac cagggcgcac cgattgccat caccgttgaa
    35641 ggggccaaca ttctgacccg cagcatgatg atcttcggac aaggagcgat tcgttgccat
    35701 ccgtacgtgc tggaagagat ggaagcagcg aagaacaatg acgtcaacgc gttcgataaa
    35761 ctgttgttca aacatatcgg tcacgtcggt agcaacaaag ttcgcagctt ctggctgggc
    35821 ctgacgcgcg gtttaaccag cagcacgcca accggcgatg ccactaaacg ctactatcag
    35881 cacctgaacc gcctgagcgc caacctcgcc ctgctttctg atgtctcgat ggctgtgctg
    35941 ggcggcagcc tgaaacgtcg tgagcgcatc tcggcacgtc tgggggatat tttaagccag
    36001 ctttacctcg cctctgccgt tctgaagcgt tatgacgacg aaggccgtaa tgaagccgac
    36061 ctgccgctgg tgcactgggg cgtacaagat gcgctgtatc aggctgaaca ggcgatggat
    36121 gatttactgc aaaacttccc gaaccgcgtg gttgccgggc tgctgaatgt ggtgatcttc
    36181 ccgaccggac gtcattatct ggcaccttct gacaagctgg atcataaagt ggcgaagatt
    36241 ttacaagtgc cgaacgccac ccgttcccgc attggtcgcg gtcagtacct gacgccgagc
    36301 gagcataatc cggttggctt gctggaagag gcgctggtgg atgtgattgc cgccgaccca
    36361 attcatcagc ggatctgtaa agagctgggt aaaaacctgc cgtttacccg tctggatgaa
    36421 ctggcgcaca acgcgctggc gaaggggctg attgataaag atgaagccgc tattctggtg
    36481 aaagctgaag aaagccgtct gcgcagtatt aacgttgatg actttgatcc ggaagagctg
    36541 gcgacgaagc cggtaaagtt gccggagaaa gtgcggaaag ttgaagccgc gtaattgaat
    36601 aacggagccg aaaggctccg tttctttatc cgctaattat ttaaaattaa agccatccgg
    36661 atggttttcc aggctgccgg tcaacgccgc gaacagcacc gttttaccat caatcgaaag
    36721 cgcatcgttc acattcagcc aggtgagttt ctcttgcgac gttttctcat caatagtcga
    36781 gaacagcccc gtcatctgat tagatttctc ggaccacatc acagcgatac gttgcgagcc
    36841 acagtcatgc ggtttgcacg cgctcatcac ctgatacgtc tcatctccca acgttacggt
    36901 ttgtgcggga gtataagtac cgcctttcat cacccaggca ggcagcttat gcccttgtac
    36961 catctgatta aatgcagctt tggtggtttc gccctttgca aggctgctaa tggttaaatc
    37021 atcctgcgcc attgcactgg tggcgatgac cagagcggcg actgtcgtta ttgccttaaa
    37081 catcattcct cccgagctta tcctgcccat gttattaacc ttctaaagcc tggcgataac
    37141 acgataattt gcaaatcaga tatttccaat atcagcattt gttatcaatt tattctgcgc
    37201 taaggcaact gcctgataag gtgttaaagt tataaaaccc ttcgcataaa ggagttgtac
    37261 accgtgcctg gtttgaagat tacgcttttg cagcaaccac tggtgtggat ggatggccct
    37321 gccaacctgc gtcattttga tcgtcaactg gaaggtatta ccgggcgcga tgtgatcgtt
    37381 ctaccggaga tgtttaccag cggctttgcc atggaagcgg cagcttcgtc gctagcacaa
    37441 gatgacgtag tgaactggat gacagctaag gcgcagcagt gcaatgcgct gattgcaggc
    37501 agtgttgcat tacaaacgga gtctggttcg gttaaccgct ttttgctggt tgagccgggc
    37561 ggcacggtac atttttatga taagcgtcat ctgttccgca tggcagatga gcatctacat
    37621 tataaagcgg gcaatgcgcg agtgattgtg gaatggcgcg gctggcgtat tttgccgctg
    37681 gtgtgctacg acttacgttt tcctgtgtgg tcgcgcaatc tcaacgatta tgacctcgcc
    37741 ctgtacgtcg ccaactggcc tgctccgcgc tctctgcact ggcaggcatt gctgacggcc
    37801 cgcgcgattg agaatcaggc gtatgtggcg ggatgcaatc gcgtcggcag cgatggcaac
    37861 ggctgccatt atcgcggtga cagccgggtg attaatccac aaggcgagat tatcgctact
    37921 gccgacgcgc atcaggcaac gcgcattgat gccgagctgt cgatggcggt gttaagggaa
    37981 tatcgcgata agtttccggc atggcgggat gcggatgagt ttaggttgta gtgagtgggg
    38041 gctgtattta gggcggggaa ttcagaaagg gatgtttagt gtgctgagcg agagt
//
LOCUS       DAZFRJ010000021        37671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000021 DAZFRJ010000000
VERSION     DAZFRJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.021"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..200
                     /locus_tag="ACHKUN_001011"
                     /pseudo
     CDS             63..200
                     /locus_tag="ACHKUN_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(308..1543)
                     /gene="aslB"
                     /locus_tag="ACHKUN_001012"
     CDS             complement(308..1543)
                     /gene="aslB"
                     /locus_tag="ACHKUN_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6659320.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1690..1766)
                     /locus_tag="ACHKUN_001013"
     tRNA            complement(1690..1766)
                     /locus_tag="ACHKUN_001013"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1730..1732),aa:Pro,seq:tgg)
     gene            complement(1809..1895)
                     /locus_tag="ACHKUN_001014"
     tRNA            complement(1809..1895)
                     /locus_tag="ACHKUN_001014"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1859..1861),aa:Leu,seq:cag)
     gene            complement(1916..1991)
                     /locus_tag="ACHKUN_001015"
     tRNA            complement(1916..1991)
                     /locus_tag="ACHKUN_001015"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1956..1958),aa:His,seq:gtg)
     gene            complement(2050..2126)
                     /locus_tag="ACHKUN_001016"
     tRNA            complement(2050..2126)
                     /locus_tag="ACHKUN_001016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2090..2092),aa:Arg,seq:ccg)
     gene            complement(2229..3614)
                     /gene="yifK"
                     /locus_tag="ACHKUN_001017"
     CDS             complement(2229..3614)
                     /gene="yifK"
                     /locus_tag="ACHKUN_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6659321.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3805..4545)
                     /gene="wecG"
                     /locus_tag="ACHKUN_001018"
     CDS             complement(3805..4545)
                     /gene="wecG"
                     /locus_tag="ACHKUN_001018"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO6659322.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4548..5900)
                     /gene="wzyE"
                     /locus_tag="ACHKUN_001019"
     CDS             complement(4548..5900)
                     /gene="wzyE"
                     /locus_tag="ACHKUN_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO6659323.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5897..6976)
                     /gene="rffT"
                     /locus_tag="ACHKUN_001020"
                     /gene_synonym="wecF"
     CDS             complement(5897..6976)
                     /gene="rffT"
                     /locus_tag="ACHKUN_001020"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO6659324.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(6973..8223)
                     /gene="wzxE"
                     /locus_tag="ACHKUN_001021"
     CDS             complement(6973..8223)
                     /gene="wzxE"
                     /locus_tag="ACHKUN_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO6659325.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8225..9355)
                     /gene="rffA"
                     /locus_tag="ACHKUN_001022"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8225..9355)
                     /gene="rffA"
                     /locus_tag="ACHKUN_001022"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO6659326.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9360..10034)
                     /gene="rffC"
                     /locus_tag="ACHKUN_001023"
                     /gene_synonym="wecD"
     CDS             complement(9360..10034)
                     /gene="rffC"
                     /locus_tag="ACHKUN_001023"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO6659327.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10012..10893)
                     /gene="rfbA"
                     /locus_tag="ACHKUN_001024"
     CDS             complement(10012..10893)
                     /gene="rfbA"
                     /locus_tag="ACHKUN_001024"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6659328.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10912..11979)
                     /gene="rffG"
                     /locus_tag="ACHKUN_001025"
     CDS             complement(10912..11979)
                     /gene="rffG"
                     /locus_tag="ACHKUN_001025"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6659329.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11979..13241)
                     /gene="wecC"
                     /locus_tag="ACHKUN_001026"
     CDS             complement(11979..13241)
                     /gene="wecC"
                     /locus_tag="ACHKUN_001026"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO6659330.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13238..14368)
                     /gene="wecB"
                     /locus_tag="ACHKUN_001027"
     CDS             complement(13238..14368)
                     /gene="wecB"
                     /locus_tag="ACHKUN_001027"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO6659331.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14424..15470)
                     /gene="wzzE"
                     /locus_tag="ACHKUN_001028"
     CDS             complement(14424..15470)
                     /gene="wzzE"
                     /locus_tag="ACHKUN_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO6659332.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15482..16585)
                     /gene="wecA"
                     /locus_tag="ACHKUN_001029"
     CDS             complement(15482..16585)
                     /gene="wecA"
                     /locus_tag="ACHKUN_001029"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO6659333.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16825..18084)
                     /gene="rho"
                     /locus_tag="ACHKUN_001030"
     CDS             complement(16825..18084)
                     /gene="rho"
                     /locus_tag="ACHKUN_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO6659334.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18169..18270)
                     /gene="rhoL"
                     /locus_tag="ACHKUN_001031"
     CDS             complement(18169..18270)
                     /gene="rhoL"
                     /locus_tag="ACHKUN_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO6659335.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18411..18740)
                     /gene="trxA"
                     /locus_tag="ACHKUN_001032"
     CDS             complement(18411..18740)
                     /gene="trxA"
                     /locus_tag="ACHKUN_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO6659336.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18871..20136
                     /gene="rhlB"
                     /locus_tag="ACHKUN_001033"
     CDS             18871..20136
                     /gene="rhlB"
                     /locus_tag="ACHKUN_001033"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO6659337.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20270..21754
                     /gene="gppA"
                     /locus_tag="ACHKUN_001034"
     CDS             20270..21754
                     /gene="gppA"
                     /locus_tag="ACHKUN_001034"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO6659338.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21801..23822)
                     /gene="rep"
                     /locus_tag="ACHKUN_001035"
     CDS             complement(21801..23822)
                     /gene="rep"
                     /locus_tag="ACHKUN_001035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO6659339.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24039..24488
                     /locus_tag="ACHKUN_001036"
     CDS             24039..24488
                     /locus_tag="ACHKUN_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO6659340.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24687..24968
                     /gene="ppiC"
                     /locus_tag="ACHKUN_001037"
     CDS             24687..24968
                     /gene="ppiC"
                     /locus_tag="ACHKUN_001037"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO6659341.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25055..26530)
                     /gene="ilvC"
                     /locus_tag="ACHKUN_001038"
     CDS             complement(25055..26530)
                     /gene="ilvC"
                     /locus_tag="ACHKUN_001038"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO6659342.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26680..27573
                     /gene="ilvY"
                     /locus_tag="ACHKUN_001039"
     CDS             26680..27573
                     /gene="ilvY"
                     /locus_tag="ACHKUN_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO6659343.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27625..29169)
                     /gene="ilvA"
                     /locus_tag="ACHKUN_001040"
     CDS             complement(27625..29169)
                     /gene="ilvA"
                     /locus_tag="ACHKUN_001040"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO6659344.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29172..31022)
                     /gene="ilvD"
                     /locus_tag="ACHKUN_001041"
     CDS             complement(29172..31022)
                     /gene="ilvD"
                     /locus_tag="ACHKUN_001041"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO6659345.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31087..32016)
                     /gene="ilvE"
                     /locus_tag="ACHKUN_001042"
     CDS             complement(31087..32016)
                     /gene="ilvE"
                     /locus_tag="ACHKUN_001042"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO6659346.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32036..32299)
                     /gene="ilvM"
                     /locus_tag="ACHKUN_001043"
     CDS             complement(32036..32299)
                     /gene="ilvM"
                     /locus_tag="ACHKUN_001043"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO6659347.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32296..33942)
                     /gene="ilvG"
                     /locus_tag="ACHKUN_001044"
     CDS             complement(32296..33942)
                     /gene="ilvG"
                     /locus_tag="ACHKUN_001044"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO6659348.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(33945..33995)
                     /gene="ilvX"
                     /locus_tag="ACHKUN_001045"
     CDS             complement(33945..33995)
                     /gene="ilvX"
                     /locus_tag="ACHKUN_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO6659349.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34082..34180)
                     /gene="ilvL"
                     /locus_tag="ACHKUN_001046"
     CDS             complement(34082..34180)
                     /gene="ilvL"
                     /locus_tag="ACHKUN_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO6659350.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
     gene            34533..36053
                     /gene="yifB"
                     /locus_tag="ACHKUN_001047"
     CDS             34533..36053
                     /gene="yifB"
                     /locus_tag="ACHKUN_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO6659351.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36078..36416)
                     /gene="maoP"
                     /locus_tag="ACHKUN_001048"
     CDS             complement(36078..36416)
                     /gene="maoP"
                     /locus_tag="ACHKUN_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO6659352.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36535..37374
                     /gene="hdfR"
                     /locus_tag="ACHKUN_001049"
     CDS             36535..37374
                     /gene="hdfR"
                     /locus_tag="ACHKUN_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO6659353.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37470..37545)
                     /locus_tag="ACHKUN_001050"
     tRNA            complement(37470..37545)
                     /locus_tag="ACHKUN_001050"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37510..37512),aa:Trp,seq:cca)
     gene            complement(37554..37630)
                     /locus_tag="ACHKUN_001051"
     tRNA            complement(37554..37630)
                     /locus_tag="ACHKUN_001051"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37594..37596),aa:Asp,seq:gtc)
BASE COUNT         9209 a        10278 c         9477 g         8707 t
ORIGIN      
        1 atctcaatta tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt
       61 gcatggaatt ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc gctcttcctc
      121 tcatggccag cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat
      181 acgatcaccc aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt
      241 aggccggata aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc
      301 cgctttttta cttactcacc accagcaaat gcgcatgcat aatgtcactg gccgggcgac
      361 cgtgcgccag caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct
      421 gatacccggc acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt
      481 gtttcgggca gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta
      541 attgcttaaa tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa
      601 ttgtttgctg atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt
      661 aaacgtcgcc gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa
      721 ccgcacccgg cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct
      781 tgccgacgtc gcgtttgatc cagtggtcga atatcgccac cagaaattca ccaaattcat
      841 cggggcgcac cgaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca
      901 gtccatcgct ggcagctgtt tcatcagcca ggcgctcgac caccggaata aactggatga
      961 attcgactcc cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc
     1021 ggttgacgca gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac
     1081 gcatcaccag cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct
     1141 cagccggacc gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc
     1201 atttatcatc gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac
     1261 cgtatttcgc ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt
     1321 cgccgccctg ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac
     1381 ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg
     1441 attgtttttc gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt
     1501 tcgccatcac atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga
     1561 agtcatcctt caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc
     1621 gtttattgcg aagggagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg
     1681 gttcaaaatt ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt
     1741 gcgctaccaa gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt
     1801 taaagtcgtg gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct
     1861 gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg
     1921 tggctaatgg gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga
     1981 gctatagcca ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc
     2041 gggactaaat ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc
     2101 gctctatcca gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt
     2161 cccacccctc tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt
     2221 tttgctgatt attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta
     2281 taaatcgccg tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct
     2341 tcattaaagt acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc
     2401 cacgggaaca ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga
     2461 aaacgcagct ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca
     2521 ctgtagacgt agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca
     2581 attagcagaa tagcaataga tactgccaca cccgcaactg gtacgccgtg acgggaaact
     2641 ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa
     2701 ctgtacatgc cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg
     2761 cccgccgctg cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg
     2821 ccaatttcat tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc
     2881 aggatccgcc acagcacttt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct
     2941 tcaccggcag taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc
     3001 gcggtcagga accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta
     3061 aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca
     3121 atcatcacga taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac
     3181 aaccgcaccg ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc
     3241 cactgcgcca tctccgggaa ccagaactgg acataaacgc caatggcggt gatttctgag
     3301 atccccaccg ccatccacat aaaccagtaa gaccaggcgg tgagataacc aaagaacggg
     3361 ctcatataac gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt
     3421 tcgcccattg aacgcatgat gaaaaagacg aatagcccgg cgatgatata ggccaacaat
     3481 acggatggcc cggcccattt caaggtactg gcggccccca taaacaggcc gacgccaatg
     3541 gtgcccccca gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt
     3601 ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc
     3661 gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag
     3721 cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc
     3781 cgtcgcttta caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg
     3841 cagcaaacga agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc
     3901 cagtcccagc gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta
     3961 agtcccgcca acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa
     4021 gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat
     4081 gcgttcaaac agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat
     4141 attcacattc cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc
     4201 gcccacaaga aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag
     4261 atcagcaccg gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac
     4321 aacgctgatg ccatccgcat atttaaattc ggcagcgtta attagctccc tgacctcagc
     4381 attatcttca atggtcagca ttttttcggc attaatggca accagcgttc cctgcttaag
     4441 ctgcccgtca gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg
     4501 taagccacgc agcgtatagg tcggtgccat ggtgttgtta ttcattgtta tccttcaacc
     4561 tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag
     4621 tacaacagtt ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt
     4681 gagacaaacg aatccagccc ttcacgtgcc agcacgatca tattgaagat cgccccaaag
     4741 cagaaactgt gcaatatcgc agccttatag cgattaggct cgcggttgcc cagctcatac
     4801 agccagtcga accatttgat gatcagacca accacgatcg ccccgagcgg gatgaacaac
     4861 gcgccgccca tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg
     4921 ttattcagca cttcccaggt aaagtagttg gctgagttca gcaccatact cgggcgaccc
     4981 ggccacagcc aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg
     5041 aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaggtg
     5101 tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag
     5161 cgttttagcg ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc
     5221 cacaacgaaa tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg
     5281 atattggcgc gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg
     5341 acgaggaaaa acagccacgc tttgctatcc tggcgcagaa agtagaccac cagcatcgcc
     5401 gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg agacttcact ggagaagatc
     5461 tgactgtagg agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg
     5521 cttaccagcg cgatgcccat caggatcacc cacgtaagat tggtctccac gcggttcatg
     5581 gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg
     5641 acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc
     5701 ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa
     5761 aaggtgagca aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac
     5821 tcaaaccagg tcagcgtggc aataaacagc gtgcagagca gccagaccac aaacaggcca
     5881 ctgaattgca gcagactcat gcgccctccc cggcggcaat cgccaacgcc cgctgccagc
     5941 cttgtagata gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc
     6001 gctgcgcttc acgcacaata tcctcgttga gatcgtcggt agtaaacagc actggcaaat
     6061 gctgttccgt catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg
     6121 cctgaatcag taagcacagc gtaccaatgc cctgctggcg agcaaaaata aagtaaccaa
     6181 gatcgcactg acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcaaaattt
     6241 gcagattttc ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt
     6301 cgttattagg cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat
     6361 gaacggcacg taaagcggcg acatgctcat tgctgaggtc gccggagttc cccaccagaa
     6421 tggtcatttt tccttcacgt tgccgatcgt tcgccatcgt attgagagaa gggtccatcc
     6481 gcgtcgggaa gtacagcagt tctccccgca cctttgggtg cgttttggca aaaaagctca
     6541 aatcgccgcg ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagtgggt
     6601 aaaaaagctt atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc
     6661 agaaaaactg gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga
     6721 actgaccgtg gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt
     6781 ccgccagcga ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta
     6841 agccgtcgtc cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcagccagcg
     6901 cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt
     6961 gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa
     7021 atagacgata taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat
     7081 tagccagtgg gcaaatacca tcaataaagt gaactggctg atttccgcca gaatataaaa
     7141 ccgcaatgac gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac
     7201 atcacccact aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag
     7261 cagccagata gcaaaatcac gcagcagcca gacggtaaaa ctcgccgccg ccactgccgg
     7321 taagacgaat ttcagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg
     7381 cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc
     7441 ggaaatactg ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag
     7501 cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag
     7561 cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa
     7621 cgggatgaca ccacgtttga tcaacatgat agtggcagga attaccacca gcgcgggaat
     7681 cagcgccaga cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc
     7741 gagaacgcca atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa
     7801 gcctttcatc agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac
     7861 taaacgcacc agcccctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc
     7921 tgccagcaca aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt
     7981 gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac
     8041 accgttaaag atgccagctc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt
     8101 tgccgccagc ccaagccccg ccggaccaaa tgacacagcc agcaacttac cgaccagtaa
     8161 cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga
     8221 catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca
     8281 ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt
     8341 caccgtggaa ctcaccaaag cgttcccccg cagggcaacc gtgcagcgga atgtaatgga
     8401 acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat
     8461 cccgcagttt aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca
     8521 gctcgatacg cccggctttc gccagaggcg ctaacgcatc gtagtagttt tgccacagcg
     8581 ccagacgttg ctggttgata cgatccgctg cttccagttg cgcccacagg tacgcagctt
     8641 gcagatcgga catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac
     8701 cacggaagaa ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta
     8761 acgctttatc gttaatcagc gtcgcgccgc cttcaccacc cgccgtatag tttttggttt
     8821 catggaagct aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg
     8881 acatcacgcc ctgagcggca tcttctacca caaacagatt atgctttttc gccaacgcca
     8941 taatggtgtc catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt
     9001 tgtcggtgat cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc gggcgaacat
     9061 ccacaaaaac gatttttgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt
     9121 agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca
     9181 gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc
     9241 actgctggca gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg
     9301 actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat
     9361 cacctgtata accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta
     9421 agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac
     9481 gcccagttta gcgccgtttg catcagctca gcacctacac cgcgtccagc cagcaggcca
     9541 attcgcgcat ctgtcgcatt gagttcccgt aaagagacat aaccgcgaat accgccggac
     9601 gccgcacgta aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac
     9661 tgtgcataaa agcgtctgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc
     9721 gcaaatgcgg cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactaca
     9781 gcaccactat cactgacatt gttcacgggt agtgccaaat caacttcacc ttctaccagg
     9841 gagaatccca gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc
     9901 tgcacccgtg accacggcgc taacacgtct ggcgtcagga gcggtgcttc agacgtaatg
     9961 cgcacgatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg
    10021 gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga
    10081 actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc
    10141 caggcaggca atcttaaagc cctggcgttt ttctactgtc tgcacaaagg tactggcttc
    10201 aatcaggctg tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac
    10261 ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg
    10321 ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc
    10381 ggtcaccgcc cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa
    10441 attgtcgtca aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaatac
    10501 cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa
    10561 gaagatgtta tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat
    10621 gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcgtattcca gctggatacc
    10681 gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat
    10741 gatgagaatt tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg
    10801 cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt
    10861 gccggaaccg cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt
    10921 cagacctaaa cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt
    10981 agccagatac cactgcaccg ttttacgcat tccactttca aaggtttcct gtggcagcca
    11041 accaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg
    11101 gtcagcgaca aaggtgatca agtcacgata gtgcgccacg ccgtgcggct tgttcggagc
    11161 cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg
    11221 accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg
    11281 ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag
    11341 cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta
    11401 aggaccgtag ttattcgagc agttggtgat cagcgtcggc agaccgtagg tccgcagcca
    11461 ggcgcgcacc agatggtcgc tactggcttt tgacgcggaa taagggctgc tcggcgcata
    11521 cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt
    11581 ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat tccagtaagc
    11641 ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg
    11701 gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg
    11761 atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc
    11821 aaagtgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt
    11881 atcgaccact accaccgcat cgctcgtttc gttgatgata taacgcacca gcgccgagcc
    11941 aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca
    12001 tcgacgacat actgctgatg gacattgtcg ccattgataa ctttgaactg actatgatcg
    12061 accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa
    12121 agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag cgtttcgccg
    12181 ctgtgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca
    12241 atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg
    12301 gtagcagcaa ggcagtcggc aaccgccgct ttcacctgat cgataaccca gaacggtttg
    12361 tgatcgttaa cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg
    12421 ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata
    12481 ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg
    12541 gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg
    12601 gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa
    12661 attttgtaca gttcgctggc gcgggtcgaa caaaccggcg tcataccacc aatcacgcga
    12721 tcgtttttaa tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca
    12781 atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct
    12841 gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc
    12901 agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata
    12961 tctggctcat gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca
    13021 actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc
    13081 aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg
    13141 atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg
    13201 atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt
    13261 ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc
    13321 tcatagtttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct
    13381 gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg
    13441 tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg
    13501 agtcggtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg
    13561 gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt
    13621 tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg
    13681 ccagcgcgtg gcagatttct tcaaagccac gaccgaaact ttcacgcctg tgaccggtca
    13741 ccagaatcat ctttttatcg gggtcgataa acgggtaatt tgccgccagt tctgaacgca
    13801 gcgtgtcgct gctcatcacc tgatcacgca cccataacag tgcatcaatg actgtattac
    13861 cagtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt
    13921 cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg
    13981 gccacggcga atagagatcg cccgtgcgta aaccagcctc aacgtgacca acaggaatac
    14041 gctgataaaa cgccgccagg ctggttgcca gcgtcgttgt tgtgtcgccg tgaaccagca
    14101 cgacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta
    14161 tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa
    14221 agagtttcag cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct
    14281 caaaaaaagg atcttttgcc aacgcatgca ccaacggagc catcttgatg gcttccgggc
    14341 gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct
    14401 ttgcgctcgc cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc
    14461 ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg
    14521 cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccaga
    14581 gttggaccaa cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag
    14641 gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg
    14701 aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta
    14761 tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc gtagatggct
    14821 ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat
    14881 gcgcctttca gctcctcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac
    14941 tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac gctgtcattg
    15001 accgcgcggg taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac
    15061 gccgcatcgg ctttgctgtt gcccaccatc cgctgcttgt aatagtcggt ttgcagccag
    15121 aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc
    15181 atgaccgatg gttggtcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat
    15241 tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc
    15301 gcgctccact cttgtcgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc
    15361 cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc
    15421 agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca
    15481 tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct
    15541 taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg aagaatgcta
    15601 gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc
    15661 caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga cgggaagtaa
    15721 acccggcacg catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc
    15781 ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc
    15841 acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg agcaggatcc
    15901 agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc
    15961 gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc
    16021 accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg
    16081 agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa
    16141 tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc
    16201 aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa
    16261 tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca
    16321 gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat
    16381 agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga
    16441 gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt
    16501 ttgccacttt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat
    16561 cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt
    16621 ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc
    16681 aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca ggaattttcc
    16741 taacatcgcc agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg
    16801 tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt
    16861 ggtcttagtc atggccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg
    16921 gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc
    16981 ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat
    17041 cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt aaataacttc
    17101 gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc
    17161 ttccacgtta cgcgccgcac cgaagaagcg tttcggacga tgaagggcat tggcgtccac
    17221 accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc gcgccagacg
    17281 agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttggcctt
    17341 ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc
    17401 aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat
    17461 cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg
    17521 cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc
    17581 gataggtgat gccagatcca gtacgcgagc ggttaaatct tcagtagaac cgttaccacg
    17641 ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt
    17701 gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg
    17761 ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa
    17821 acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc
    17881 tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat
    17941 atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat
    18001 acgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt
    18061 attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga
    18121 acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat
    18181 gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag
    18241 tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta
    18301 caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc
    18361 acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg
    18421 ttagcgtcga ggaactcttt caactgacct ttagacagtg cacccacttt ggttgccgcc
    18481 acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca
    18541 gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca
    18601 gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa
    18661 tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg
    18721 tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt
    18781 tgttggtgta acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa
    18841 agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag
    18901 ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat
    18961 aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc
    19021 gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat
    19081 cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccacgtgc cttaattatg
    19141 gcaccgacgc gtgaacttgc cgtgcagatc catgccgacg cagaaccgct ggcggaagct
    19201 accggcctga agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg
    19261 ctggaaagcg gcgttgacat tctgattggc actacggggc gtttaattga ctacgccaag
    19321 cagaaccaca ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg
    19381 tacgatctgg gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac
    19441 cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc
    19501 gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt
    19561 attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg
    19621 atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaata ccgttgtgaa
    19681 gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc
    19741 gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg
    19801 gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac
    19861 tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca
    19921 ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa tttgcctgct
    19981 attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg
    20041 accgatctgc caaaaccgct gcgcctcacg cgcccacgta caggcaatgg tccgcgtcgt
    20101 actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac tcaaaatcat
    20161 tcaaactgta tcaagatggc aagagaatga atcccgatga gcatactgta gtaagtgatt
    20221 caggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta tgggttccac
    20281 ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg
    20341 cgaggtggct ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc gtctggctgc
    20401 tggcctgaat agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt ggcaatgtct
    20461 gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg ttgtcgctac
    20521 ggcgacatta cgtcttgccg tcaatgcggg tgattttatt gccaaagcgc aggaaatcct
    20581 cggttgtccg gtacaggtga tcagcggtga agaggaagca cgtctcattt atcagggcgt
    20641 tgctcacaca actggcggtg ccgatcagcg cctggtggtg gatataggcg gtgccagtac
    20701 tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg
    20761 cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaggaaa attttgatgc
    20821 tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac ggtatcacgg
    20881 ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc
    20941 acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac agcgagccat
    21001 tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac gtgcgttagt
    21061 gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata ttcagtgtat
    21121 gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc atctggccgt
    21181 cgagcaggat attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat
    21241 tgatcaggcg cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg tggaaaagga
    21301 atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc ttcatgaaat
    21361 cggcctgagc gttgacttta aacaagcgcc gcaacacgct gcttatctgg tgcgtaatct
    21421 ggatcttccc ggttttaccc ccgcacagaa aaaattgctg gcgacgctac tgctcaacca
    21481 gactaatccg gtcgatctct catcgctgca tcagcaaaat gccgtacctc cgcgcgtcgc
    21541 agaacaactc tgccgtttac tccgcctggc catcattttt gccagccgtc gccgtgacga
    21601 tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca
    21661 aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa gccagtggca
    21721 gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga
    21781 cgcatctttt ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcagattc
    21841 gccagatggc tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt gcgctcctgt
    21901 tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc cgggcgcacc
    21961 agctcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcgg
    22021 gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc
    22081 tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa ctccagccct
    22141 tttgacgcgt gcagagtcat cagttgcacc tgatccagct cttcttcact ctcaccacgc
    22201 tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc
    22261 agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg
    22321 cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg
    22381 ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg ctggatttct
    22441 gccaaccagt gagtaaagcg ggtcaatgct tcataaccac gtccgctaag cgtctgactc
    22501 aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccactcaccc
    22561 agctttttca gcgtcgccgg gccaatctct ctcttcggcg tgttaacgat acgcagaaat
    22621 gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc cttgatttca
    22681 ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg
    22741 aatttttcaa acactcgcga ctgatggtta ccgcgataaa gaatagcgta atctttgtac
    22801 tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc ggcttcatgt
    22861 tcttcgttat tcgcgcttaa aactttaagc tctgtgccgt aacccagttc ggagaacaga
    22921 cgcttctcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt caaaatacgt
    22981 ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa atcctgactc
    23041 agcagcacca ggttttgtgg acgtgcgccg cgccaggagt agatcgactg gtcgtcatcg
    23101 cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc atactggctg
    23161 gtgttggtgt cctgatactc atccaccagc agatagcgaa ttttgttctg ccagcgctcg
    23221 cggacttctt cattgcgttg cagcagcaac gtcggcaata aaatcagatc atcgaagtcg
    23281 agaacattgc acgctttcag gtgtgcatca tacagcccat aacaatgggc aaaaatacga
    23341 tcccgctcgc cctttgcctc tgccgccgcc tgcgccggag ttttgagatc gttcttccag
    23401 ttggagatgg tcgaaatcag ctgttgcagg agaactttgt catcttcaat cagcccctcg
    23461 gtcaactctt taagcaaagc aagctgatcg gtatcgtcaa acagcgaaaa gttcgccttc
    23521 atcccaagcg ccgcatactc gcgtttgatg atatccaacc ccagcgtgtg aaaagtggag
    23581 atcatcaacc cgcgcgcctc tttgcgaccc agcgtctgcc cgacacgctc tttcatctcg
    23641 cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata accgcaaccg
    23701 cggatcagat gggcgatttt attggtgatc acacgagttt taccggaacc cgcgcccgcc
    23761 agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg gtttagacgc
    23821 ataggtgtat tgctcaatct tcgaacgggg ggaggattgt agcagaatcg catttgaatg
    23881 tgaccgcgtg taagccgcta accggttggc tgcggatgtt taaatcagag agagaagacc
    23941 gaaatcggag ttcatcgtga atccattaga cttagaaaat atcgggtcgt aacgttgtgt
    24001 tattactttt tcagagttta ctaaggagag gtttactcgt ggcgattaat ttcagcccta
    24061 aggttggtga aatacaggaa tgcaactttg gtaactatcc tgttatccag aatggacagt
    24121 tcagcaccac gtattacgat ggccgcattc ctcctgaaat gataaaaaac aggttagttg
    24181 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
    24241 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
    24301 gcctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctggta caacaagtca
    24361 gtcgaaaccg attaaataga gcaaggactt acagagggta tcttaatcaa tgtttgccac
    24421 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
    24481 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
    24541 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
    24601 cagcccctcc ctgactgaca attacgttag catctgcgga tcatgctaaa atcgccgcct
    24661 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
    24721 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
    24781 gcaagctggc gaagaaacac tctatttgcc cgtcaggcaa acgcggcggt gatttaggtg
    24841 aattccgcca gggtcagatg gttccggcgt tcgataaagt ggttttctct tgtccggtac
    24901 tggagccgac cggcccgctg cacacccagt tcggatatca catcattaag gtgctgtacc
    24961 gcaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
    25021 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaacagca atacgtttca
    25081 tatctgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg ctgcgaatcg
    25141 cttcgttcac atcacgcagt tgcgcgttat ctaccgcgcc ttccggaata gctttaccca
    25201 agtcgcccgg ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga
    25261 acagatagtt accgtactca gcggtatcag agataaccac gttcatttcg tacagacgct
    25321 tacgggcgat ggtgttggca atcagcggca gctcgtgcag tgattcataa tatgcagact
    25381 cttcaatgat gccggaatcg accatggttt cgaacgccag ttcaacgccc gcttttacca
    25441 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
    25501 gcggcgcggt ttcaaacgcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
    25561 catcgttggc ccagtccgcc atcataccgg aagagaattc gccggagatg atgtcgtcca
    25621 tatgtttctg gaacagcggt gccatgatct ctttcagctg ttctgacagc gcataagcac
    25681 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
    25741 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
    25801 cctcttccac cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga
    25861 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
    25921 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccata ccttcgcctt
    25981 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
    26041 cttcacgcac ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atatctttac
    26101 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
    26161 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtcc ggcgtcaggt
    26221 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
    26281 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
    26341 agatatccag accagaatca cgcatgttca ggccctggtt cagaccctgt gcgccacagc
    26401 cgacgatgac tactttttta ccctgaaggt agctcgcgcc atcggcgaat tcatcgcgcc
    26461 ccataaagcg acatttgccc agctgtgcca gctgctgacg cagattcagt gtattgaagt
    26521 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
    26581 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
    26641 attgcaattt ttgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
    26701 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
    26761 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
    26821 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
    26881 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
    26941 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
    27001 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
    27061 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
    27121 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
    27181 ctgattgcgc ccgcgctgcc ctgcccggtg cgtaaccagg tttcggcaga gaagccggac
    27241 tggtctacag tgccgtttat catggccgat cagggaccgg tgcgtcgccg cattgaactg
    27301 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
    27361 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtagtgctg
    27421 gaaaacagcc ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgaaaaa
    27481 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
    27541 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
    27601 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccgggttatt
    27661 ggtttcgtcg tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg
    27721 gtcgccaagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
    27781 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
    27841 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
    27901 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
    27961 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
    28021 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
    28081 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
    28141 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
    28201 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt
    28261 gtgctgggcg atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac
    28321 cgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
    28381 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcaccga tgcgttttac
    28441 cgctacgcct tcagcaaata gccctacgcg cggcagatca accggatgac ccgcatccag
    28501 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
    28561 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
    28621 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
    28681 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
    28741 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
    28801 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
    28861 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
    28921 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
    28981 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
    29041 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
    29101 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
    29161 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
    29221 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
    29281 gtccaggctt tgtcacctcg agcgtcctgc gcttcacgac gcgccgccag ttcggcatcg
    29341 cttacctgta actgaatgcc acggttcggg atgtctatag cgatcaggtc accatcttca
    29401 atcaggccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
    29461 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
    29521 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
    29581 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
    29641 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
    29701 acgcccgcgg ttttaacgat gcagccgttt tctgcgaaat taccgtagag caccgccagg
    29761 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccattggc gcgatcgtca
    29821 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tggtacgaat gcctgcagga
    29881 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
    29941 agcgtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
    30001 cgatccagtt cgccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
    30061 tatttctggg tgctcggcgc aactttacac agctgtggaa ccttgcggga aagcttatcg
    30121 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacg
    30181 gtgttagtcg atccacccat cgcgatatcc agcgtcatgg cgttttcaaa cgccgctttg
    30241 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
    30301 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtcggcgtg ggttgccagc
    30361 agcgagccgt tgcccggctg cgacagaccc agcgcttcgg tcaggcagtt cattgagtta
    30421 gcggtaaaca tcccggagca ggagccgcag gtcggacacg cggaacgttc aacctgatcg
    30481 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagt
    30541 ttgattatct gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag
    30601 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
    30661 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
    30721 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat ggcaatccca
    30781 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
    30841 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
    30901 ttcacaaccg cgataatcgg cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac
    30961 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
    31021 atactttatt tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt
    31081 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
    31141 gaggccgaag aaggcttgct gaatgcgttt ggtaaccggg ccacaacggc cttcgccaac
    31201 ctgaatacca tctacactgc gcactggcgt gatttctgcc gcagtaccgg acataaacac
    31261 ttcatccgcc aggtacaggg attcgcgcga cagaacctgc tcacgcactt caattcccag
    31321 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa
    31381 cggtggggtg aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga
    31441 gatataaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
    31501 acccaccagc agggaagaga gatagttacc accggctttt gccgcggttg ggatggtgtt
    31561 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
    31621 gcccagatac gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg
    31681 cgggtttaca cccatgccaa catcaccaac gaagatcagc ggacggatat aggcgctggt
    31741 gagattgttt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
    31801 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
    31861 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
    31921 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
    31981 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
    32041 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
    32101 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
    32161 tatttatatt ttgtgcatcg ctggcggcag ccatattcat tgagcagacg tggaaaccac
    32221 gatgacgcac cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat
    32281 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
    32341 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
    32401 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
    32461 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
    32521 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
    32581 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
    32641 attcatcatg aaagagccgt caccggagat acagacgaca gtatcgttcg gtcgtgcgac
    32701 ttgtgcgcca accgccgctg gtaaaccgaa acccatggtg cctaagccgc tggaggtaat
    32761 gaaattttcc gggcgagtgt gtgcgatgtg ctgcgcggcc cacatctggt gctgccccac
    32821 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
    32881 cgcgtagata gcgtcaccgg gatggtcgta acgccaggta tgttcatcac gcagctgcgc
    32941 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
    33001 taaatcacct tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
    33061 atccatatgg ataacactgg cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc
    33121 atcaaaacgt gcacccacgg cgatcagcaa gtcgcactcc tgcaccgcga agttcgccgc
    33181 tttggtgcca tgcattccca gcatgcccag gtagtacgga taatctgctt caactgcgcc
    33241 cagccctttc agcgtgcagg tggcaggcat ttttgtggta gcgagaaatt ctcgtaaagc
    33301 aggaactgcc tgcgccatac ccacgccacc accaacgtac agcatcggtt tttgcgcttt
    33361 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
    33421 ggtggtgaac cacggttcca ggtcaccgct ggctagctgg atatcttttg ggatatcgac
    33481 cagaaccgga ccaggacgac ctgagctggc aacgtcgaat gcttcagcca taatgcgcgg
    33541 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcccag
    33601 gacatccact tcctgaaatg cgtccgtgcc gataaacggt gcggacactt gaccggtgat
    33661 ggcaacaaca gggatagaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
    33721 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
    33781 agccgccatt gccgcaccct gctcatgtcg gcacagcaag tgctccacgc cgccgtcata
    33841 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
    33901 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
    33961 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatattttta
    34021 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
    34081 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
    34141 taatcatgac caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    34201 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atattttttg
    34261 gccgattttt atgatgcgtt taataatttc aatagaaatt gttgtttttt aaaaagaaaa
    34321 tagtgagtga aaaaaattca atttcagtat gaatgttgtt cccttcctcg tagttcatcc
    34381 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    34441 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    34501 ttatgcacat aatagtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    34561 cagccctggg agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtctac
    34621 ccggcttaac gatggtgggc ttaccagaaa caactgtaaa agaagctcgc gatcgcgtgc
    34681 gcagcgccat tatcaatagc ggatatgaat atccggcgaa aaaaatcacc atcaacctgg
    34741 ctccagccga tctgccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc
    34801 tggcggcctc agaacagctt acagccaata agttagatga atatgaatta gtcggagaac
    34861 tggcgcttac aggcgctctg cgtggcgttc ccggcgcaat ctccagtgca actgaagcta
    34921 ttaagtcggg cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta
    34981 acggtgaagg atgcctgata gccgatcatc tgcaggctgt ctgtgcgttt ctggaaggta
    35041 agcacgctct cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc
    35101 tcagtgatgt tgtcggtcag gagcaaggaa agcgaggact ggaaattacc gccgctggcg
    35161 ggcacaacct tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta
    35221 ttaatggcct gttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa
    35281 gtctggtaaa tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcaccgc
    35341 atcacagtgc gtcgttaact gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa
    35401 tttcgttggc gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta
    35461 cactggatgc cttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag
    35521 ccaaaataac ctatccagcc cgtttccagc ttgttgcggc gatgaatccc agccctaccg
    35581 gacattatca gggaaaccat aaccgctgca cgccagaaca gacattacgt tatctcaacc
    35641 ggctctcggg gccctttctc gaccgcttcg atctctcact ggagatccca ttaccacccc
    35701 ccggcatttt gagtaaaacg gtagtgccgg gagaaagcag cgccaccgtt aaacaacgcg
    35761 taatggccgc cagagagcgc caatttaagc ggcagaataa actgaatgcc tggctggata
    35821 gtccggaaat acgccaattc tgcaaacttg agagcgaaga tgcgatgtgg ttggaaggaa
    35881 cactgatcca tctggggtta tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa
    35941 ccattgctga tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta
    36001 gctatcgtgc gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaagg
    36061 gcatttcgcc ctttttatta atcatcagaa tcggtgtagt cttcagcacc ttcaacctgc
    36121 ggtttaccgc cggaaagggt gtgaaaacgt tttggacgct taatacgcgt catatacttg
    36181 gaccacacgc gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag
    36241 agtttctctt cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa
    36301 ccatgacgct caagcagttg tgcctctttg atggtgaaat caccatgacg agagaatcca
    36361 cgtggataat gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg
    36421 cacgctccta attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag
    36481 agtgtaaaac aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat
    36541 acggaattgt taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct
    36601 gaatcgctct atctgaccca gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa
    36661 ctgggtgtga accttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa
    36721 aaactactgc cttatgcaga aacgctcatg agcacctggc aggccgcccg caaggaggtg
    36781 gcgcatacct cacgacataa cgagttttct atcggtgcca gcgcctcatt gtgggaatgt
    36841 atgcttaatc agtggctggg acgtttgtat caaaatcagg atgcccatac aggcttacag
    36901 ttcgaagcgc gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt
    36961 gatcttctta ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctggga
    37021 tatttcactt tagcgcttta taccagtgcc ccttcaaaac taaagggaga tcttaattat
    37081 ctgcgacttg agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac
    37141 gaagtgccca ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt
    37201 aatggttgca cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt
    37261 gtcgatagca caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa
    37321 aatacgttga ttcgcgatct tttgaaaatt aacgtgctgg atgaagtgta ttaatatgaa
    37381 tggctggcaa ggatgccggt agaaggattt acttcggaga gggttatttc agataaaaaa
    37441 aatccttagc tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac
    37501 acccggtttt ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga
    37561 acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa
    37621 ctaccgctcc accgaattct tttacaacca ccggttttat gaccggctta c
//
LOCUS       DAZFRJ010000022        37442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000022 DAZFRJ010000000
VERSION     DAZFRJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.022"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..725
                     /locus_tag="ACHKUN_001052"
     CDS             54..725
                     /locus_tag="ACHKUN_001052"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO6659354.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            735..1931
                     /gene="yaiP"
                     /locus_tag="ACHKUN_001053"
     CDS             735..1931
                     /gene="yaiP"
                     /locus_tag="ACHKUN_001053"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6659355.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1909..2490
                     /locus_tag="ACHKUN_001054"
     CDS             1909..2490
                     /locus_tag="ACHKUN_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6659356.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2492..3265
                     /gene="yaiO"
                     /locus_tag="ACHKUN_001055"
     CDS             2492..3265
                     /gene="yaiO"
                     /locus_tag="ACHKUN_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO6659357.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3451..3726
                     /gene="frmR"
                     /locus_tag="ACHKUN_001056"
     CDS             3451..3726
                     /gene="frmR"
                     /locus_tag="ACHKUN_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO6659358.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3761..4870
                     /gene="frmA"
                     /locus_tag="ACHKUN_001057"
     CDS             3761..4870
                     /gene="frmA"
                     /locus_tag="ACHKUN_001057"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO6659359.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4963..5796
                     /gene="fghA"
                     /locus_tag="ACHKUN_001058"
     CDS             4963..5796
                     /gene="fghA"
                     /locus_tag="ACHKUN_001058"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6659360.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5921..6460)
                     /gene="yaiL"
                     /locus_tag="ACHKUN_001059"
     CDS             complement(5921..6460)
                     /gene="yaiL"
                     /locus_tag="ACHKUN_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO6659361.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6562..7773)
                     /gene="mhpT"
                     /locus_tag="ACHKUN_001060"
     CDS             complement(6562..7773)
                     /gene="mhpT"
                     /locus_tag="ACHKUN_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO6659362.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8045..9058)
                     /gene="mhpE"
                     /locus_tag="ACHKUN_001061"
     CDS             complement(8045..9058)
                     /gene="mhpE"
                     /locus_tag="ACHKUN_001061"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO6659363.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9055..10005)
                     /gene="mhpF"
                     /locus_tag="ACHKUN_001062"
     CDS             complement(9055..10005)
                     /gene="mhpF"
                     /locus_tag="ACHKUN_001062"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO6659364.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10002..10811)
                     /gene="mhpD"
                     /locus_tag="ACHKUN_001063"
     CDS             complement(10002..10811)
                     /gene="mhpD"
                     /locus_tag="ACHKUN_001063"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO6659365.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10821..11687)
                     /gene="mhpC"
                     /locus_tag="ACHKUN_001064"
     CDS             complement(10821..11687)
                     /gene="mhpC"
                     /locus_tag="ACHKUN_001064"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO6659366.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11705..12649)
                     /gene="mhpB"
                     /locus_tag="ACHKUN_001065"
     CDS             complement(11705..12649)
                     /gene="mhpB"
                     /locus_tag="ACHKUN_001065"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO6659367.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12651..14315)
                     /gene="mhpA"
                     /locus_tag="ACHKUN_001066"
     CDS             complement(12651..14315)
                     /gene="mhpA"
                     /locus_tag="ACHKUN_001066"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO6659368.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDETLRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14506..15339
                     /gene="mhpR"
                     /locus_tag="ACHKUN_001067"
     CDS             14506..15339
                     /gene="mhpR"
                     /locus_tag="ACHKUN_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO6659369.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15416..16498
                     /gene="lacI"
                     /locus_tag="ACHKUN_001068"
     CDS             15416..16498
                     /gene="lacI"
                     /locus_tag="ACHKUN_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO6659370.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16621..19695
                     /gene="lacZ"
                     /locus_tag="ACHKUN_001069"
     CDS             16621..19695
                     /gene="lacZ"
                     /locus_tag="ACHKUN_001069"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO6659371.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19747..21000
                     /gene="lacY"
                     /locus_tag="ACHKUN_001070"
     CDS             19747..21000
                     /gene="lacY"
                     /locus_tag="ACHKUN_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO6659372.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21079..22260)
                     /gene="cynX"
                     /locus_tag="ACHKUN_001071"
     CDS             complement(21079..22260)
                     /gene="cynX"
                     /locus_tag="ACHKUN_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO6659373.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22266..22736)
                     /gene="cynS"
                     /locus_tag="ACHKUN_001072"
     CDS             complement(22266..22736)
                     /gene="cynS"
                     /locus_tag="ACHKUN_001072"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO6659374.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22767..23426)
                     /gene="cynT"
                     /locus_tag="ACHKUN_001073"
     CDS             complement(22767..23426)
                     /gene="cynT"
                     /locus_tag="ACHKUN_001073"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO6659375.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23536..24435
                     /gene="cynR"
                     /locus_tag="ACHKUN_001074"
     CDS             23536..24435
                     /gene="cynR"
                     /locus_tag="ACHKUN_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO6659376.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24475..25758)
                     /gene="codA"
                     /locus_tag="ACHKUN_001075"
     CDS             complement(24475..25758)
                     /gene="codA"
                     /locus_tag="ACHKUN_001075"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO6659377.1"
                     /translation="MSNNALQTIINARLPGKEVLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25748..27007)
                     /gene="codB"
                     /locus_tag="ACHKUN_001076"
     CDS             complement(25748..27007)
                     /gene="codB"
                     /locus_tag="ACHKUN_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6659378.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27346..29232)
                     /gene="prpE"
                     /locus_tag="ACHKUN_001077"
     CDS             complement(27346..29232)
                     /gene="prpE"
                     /locus_tag="ACHKUN_001077"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO6659379.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29272..30723)
                     /gene="prpD"
                     /locus_tag="ACHKUN_001078"
     CDS             complement(29272..30723)
                     /gene="prpD"
                     /locus_tag="ACHKUN_001078"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO6659380.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30757..31926)
                     /gene="prpC"
                     /locus_tag="ACHKUN_001079"
     CDS             complement(30757..31926)
                     /gene="prpC"
                     /locus_tag="ACHKUN_001079"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO6659381.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(31971..32861)
                     /gene="prpB"
                     /locus_tag="ACHKUN_001080"
     CDS             complement(31971..32861)
                     /gene="prpB"
                     /locus_tag="ACHKUN_001080"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO6659382.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33100..34686
                     /gene="prpR"
                     /locus_tag="ACHKUN_001081"
     CDS             33100..34686
                     /gene="prpR"
                     /locus_tag="ACHKUN_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO6659383.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(34784..35059)
                     /gene="yahO"
                     /locus_tag="ACHKUN_001082"
     CDS             complement(34784..35059)
                     /gene="yahO"
                     /locus_tag="ACHKUN_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO6659384.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35206..35877
                     /gene="yahN"
                     /locus_tag="ACHKUN_001083"
     CDS             35206..35877
                     /gene="yahN"
                     /locus_tag="ACHKUN_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO6659385.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(35894..36139)
                     /gene="yahM"
                     /locus_tag="ACHKUN_001084"
     CDS             complement(35894..36139)
                     /gene="yahM"
                     /locus_tag="ACHKUN_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO6659386.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(36551..37366)
                     /gene="yahL"
                     /locus_tag="ACHKUN_001085"
     CDS             complement(36551..37366)
                     /gene="yahL"
                     /locus_tag="ACHKUN_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO6659387.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT         8787 a        10145 c         9725 g         8785 t
ORIGIN      
        1 tctgagtgca tcgttgtgct tgtagtataa taaataaaat ggagtgtcat taagtggata
       61 aggttttaga ttcagccctc ccttcttcgg caaataaaag aaagggtatc cttgctattg
      121 gcgcacatcc tgacgatata gaattaggct gtggcgcatc gcttgctcgt cttgcgcaaa
      181 aaggaattta tatcgcagcc gtggtgatga ctaccggcaa ctctggtaca gatggaataa
      241 tcgatcgcca tgaagaatcg cgcaacgccc taaagatatt agggtgccac caaactattc
      301 atcttaattt tgctgacacc cgcgctcatt tacagctcaa tgatatgatt tccgctctgg
      361 aagacatcat taaaaatcaa attccttctg atgttgaaat catgcgggta tataccatgc
      421 atgatgccga ccgccatcag gatcatctcg ctgtttatca agcttcaatg gttgcctgcc
      481 gcactattcc acaaattctc ggctacgaaa ccccaagtac ctggctttca tttatgcctc
      541 aggtttttga atccgttaaa gaagaatatt tcacagtcaa acttgctgca ttaaaaaaac
      601 ataaaagcca ggaacgacgt gattatatgc gtcatgatcg cctgcgtgca gttgcacaat
      661 ttcgcgggca acaggtcaat agcgatctgg gtgaaggctt tgtaattcat aaaatgattc
      721 tttgagcaaa ttgcatgaaa acctggatat ttatctgtat gtctatagca atgttgctat
      781 ggtttttaag tacgctaaga cgtaaaccca gtcaaaagaa aggctgtatt gacgccatta
      841 tacctgcgta taacgaaggc ccgtgtctgg cgcagtcact ggataatcta ctgcgtaacc
      901 cttatttttg ccgggtaatt tgcgttaacg acggctccac ggacaatacc gaagcggtca
      961 tggcggaagt caaacgcaaa tggggcgacc gctttgttgc cgtcacgcaa aaaaataccg
     1021 gtaaaggtgg tgcgctgatg aatggcctca attatgccac ctgcgaccag gtttttttaa
     1081 gtgatgccga cacctatgtt ccgcccgatc aagacggaat gggctatatg ctggcagaaa
     1141 ttgagcgcgg tgctgatgcc gtaggcggca ttccctctac tgcgttgaaa ggcgcggggc
     1201 tgttaccgca catccgcgcg accgtaaagt tgccgatgat tgttatgaag cgcacgctac
     1261 agcagctcct gggcggcgca ccgtttatta tcagcggtgc ctgcggaatg ttccgtactg
     1321 atgtattgcg taagttcggt ttctcggatc gtactaaagt cgaagacctt gatctcacct
     1381 ggacattagt ggcaaacggc taccgtattc ggcaggcgaa tcgctgcatc gtatacccac
     1441 aggaatgcaa cagcccgcgt gaggagtggc gtcgctggcg gcgttggatt gtgggatacg
     1501 cggtctgtat gcgcctgcat aaaagacttt tatttagccg cttcggtatc ttcagtatat
     1561 ttcctatgct gttggttgtg ctttatggcg ttgggattta tctcactacc tggtttaatg
     1621 aattcatcac caccgggccg catggtgtgg tgttggcaat gtttccgctt atctggatcg
     1681 gcgtagtttg tgttattggt gcttttagcg cctggtttca tcgttgctgg ttgttggtgc
     1741 ctttagcgcc gctttccgtt gtgtatgtat tattagctta tgccatctgg attatttatg
     1801 gacttattgc cttttttact ggacgcgaac ctcagcgcga caaacccacc cgctattccg
     1861 cactggtgga agcgtcaacc gcttattccc aaccttctgt cacaggaact gaaaaactat
     1921 ctgaagctta atgttaaaga gaaaaatatt cagattgcag accaggtaat tattgatgaa
     1981 actgcaggtg aagttgttat cggcgcgaat acccgtattt gtcatggtgc cgttattcag
     2041 ggtccggtag tgattggcgc aaactgcctg ataggtaatt atgcgtttat tcgtcctggc
     2101 acaataatca gcaatggtgt aaaaattggt tttgccaccg aaattaaaaa tgcggttatt
     2161 gaagcggaag caacgattgg tccgcaatgt tttattgccg actcggtagt tgcaaaccag
     2221 gcatatttgg gcgcacaagt acgtaccagt aatcatcgtc tggatgaaca gcctgtgtct
     2281 gttcgaactc cagagggaat tatcgctacc ggatgcgata aattaggttg ttatatcggg
     2341 cagcgttcac gccttggcgt acaagttata attttgcctg ggcgaattat ttccccgaac
     2401 acgcaacttg gcccgcgcgt gattgtagaa cgtaatttac ctgccggaac ttattcactc
     2461 cgacaagaac ttatccgtac aggagattaa aatgataaaa cggacgttat tagcggcggc
     2521 catttttagc gcattgcccg cttatgccgg gttaacttcc attaccgcgg gctacgattt
     2581 taccgattat tctggcgatc atggcaaccg taatttagcg tatgctgaac tggtggcgaa
     2641 agttgaaaac gcaacgctgc tttttaatct ttcccagggg cgtcgtgatt atgaaactga
     2701 acatttcaat gccactcgcg gtcagggtgc ggtctggtat aaatggaata actggctgac
     2761 aacccgaacg ggtattgcct ttgcggataa tacgccggtc tttgcccgcc agaattttcg
     2821 tcaggatatt aacctggccc tgttgccaaa aacgcttttc acgaccggtt atcgctacac
     2881 taaatattac gatgatgtcg aagtcgatgc ctggcaaggc ggcgtatcac tctatactgg
     2941 cccggtgatc accagctacc gctataccca ttatgactcc agcgatgcag gtggtagtta
     3001 tagcaatatg atttccgtgc gcctgaatga cccgcgcggc actggttata cgcaactatg
     3061 gctaagccgc ggaacaggcg cttacaccta tgactggacg ccagaaacac gctacggcag
     3121 catgaagagc gtcagtctgc aacgtattca accgctgact gagcaactta atctcgggct
     3181 gacggcaggt aaagtgtggt acgacacccc aaccgatgat tataacggtc tgcaacttgc
     3241 agcccatctg acctggaaat tctgattcct tctgccgccc gctatccggg gcggccttcc
     3301 ctgccgatta gcccccccct ttcctctttg ttttccgacc acattcaccg gataaatttt
     3361 attctccagt gttatatact ataggggggt atgcattgac atgtagtata cccccctata
     3421 gtatattgcg tgcagatgat gaggtgcgaa atgcccagta ctccggaaga gaagaaaaag
     3481 gtccttactc gagttcgtcg tattcgaggg cagattgatg ctctggaacg gtcgatggag
     3541 ggtgatgccg aatgccgtgc catactccaa cagatcgctg ccgttcgggg cgcggctaac
     3601 gggctgatgg cagaagtgct tgaaagccat atccgggaaa cgtttgaccg aaatgactgc
     3661 tacagccgcg aagtcagcca atccgttgac gacactattg aactggttcg agcctatctt
     3721 aaatagctga atctattacc atattgagga agagcgagag atgaaatcac gtgctgccgt
     3781 tgcatttgct cccggtaaac cgctggaaat cgttgaaatt gacgttgcac caccgaaaaa
     3841 aggtgaagtg ctgattaaag tcacccatac cggcgtttgc cataccgacg catttaccct
     3901 ctccggggat gacccggaag gtgtattccc ggtggttctc ggtcacgaag gggccggcgt
     3961 tgtggttgaa gtcggtgaag gcgtaaccag cgtcaaacct ggcgaccatg tgatcccgct
     4021 ttacaccgcg gagtgcggcg agtgtgagtt ctgtcgttct ggcaaaacta acctctgtgt
     4081 tgcggttcgc gaaacccagg gtaaaggctt gatgccagac ggcaccaccc gtttttctta
     4141 caacgggcag ccgctttatc actacatggg gtgctctaca ttcagtgaat acaccgtggt
     4201 cgcggaagtg tctctggcca aaattaatcc agaagcaaac catgaacacg tctgtctgct
     4261 gggctgtggc gtgaccaccg gtattggcgc ggtgcacaac acagctaaag tccagccagg
     4321 tgattctgtt gccgtgtttg gtcttggcgc gattggtctg gcagtggttc agggcgcgcg
     4381 tcaggcgaaa gcgggacgga ttatcgctat cgataccaac ccgaagaaat tcgatctggc
     4441 gcgtcgcttc ggtgctaccg actgcattaa cccgaatgac tacgacaaac cgattaaaga
     4501 tgtcctgctg gatatcaaca aatggggtat cgaccatacc tttgaatgca tcggtaacgt
     4561 caacgtgatg cgtgcggcgc tggaaagtgc gcaccgcggc tggggtcagt cggtgatcat
     4621 cggggtggca ggttctggtc aggaaatctc cacccgtcca ttccagttgg tcactggtcg
     4681 cgtatggaaa ggttccgcgt ttggcggcgt gaaaggtcgt tcccagttac cgggtatggt
     4741 tgaagatgcg atgaaaggtg atatcgatct ggaaccgttt gtcacgcata ccatgagcct
     4801 tgatgaaatt aatgacgcct tcgacctgat gcatgaaggc aaatccattc gaaccgtaat
     4861 tcgttactga tttcccgcag gtataccccg tccacttcag acggggttct taatactctc
     4921 cctgggcagc cgtccggggg attaaccgtg agataatgac tgatggaact cattgaaaaa
     4981 catgccagct ttggcggctg gcaaaatgtg tatcggcatt attcccaatc actgaaatgt
     5041 gaaatgaatg tcggcgtcta tctcccacca aaagccgcga atgaaaaatt gccggtgctg
     5101 tactggcttt cgggcctgac ctgcaacgag cagaatttca ttactaaatc ggggatgcag
     5161 cgttacgcgg ctgaacacaa cattattgtt gttgcgccgg acaccagtcc gcgaggcagt
     5221 catgtcgcag atgccgaccg ttacgatctc gggcaaggtg ccgggtttta cctgaacgca
     5281 acgcaagcgc cgtggaatga acattacaaa atgtatgact atatccgcaa cgagctgccg
     5341 gatttagtga tgcaacattt tccggcaacg accagaaagt ctatctctgg tcattctatg
     5401 ggcgggctgg gcgcgctggt gctggcgtta cgtaacccag atgaatatgt cagcgtctcg
     5461 gcgttttcgc ccattgtctc cccatcgcaa gtaccgtggg gacagcaagc ctttgccgca
     5521 tatcttggtg aaaataaaga tgcctggttg aattacgatc cggtgagtct tatttcacaa
     5581 ggtcaacgcg ttgcggaaat catggttgat caggggttga gtgatgattt ttacgcggaa
     5641 cagctgcgga ctccaaatct tgaaaagatc tgccaggaga tgaatatcaa gacgttaatc
     5701 cgttatcacg agggttacga ccacagctat tattttgtct ccagttttat tggcgagcat
     5761 attgcctacc acgccaataa actgaatatg cgttgataat agtgcacgac tgccggatgc
     5821 ggcgtgaacg ccttatccgc ctacatttcg cccgcaggcc gtaggcatga taagacgcgc
     5881 atagcgtcgc atcaggcatc tgcgcacgac ttatctatgt ttaccacatc aaatcatcag
     5941 gcactttgaa gtcggcatat gggtcgtctt catcctgctc ttcggcgctg agcgcgctgt
     6001 gcaacactat actgctggca tcacgctgag ctattttatc ggcaacgctg gcgggaataa
     6061 tggcgtattc accttcgcta ttgttatcga ccaacaagcg agcaatcgcc agacgaccat
     6121 taatcaactg cgcctgtgtg agcttatcga caaaaatctt cttaatcaga ttgccgtcag
     6181 tgaagttaaa accaatatcg ccattggcaa tggtgattcg gttcatttca atgagctgct
     6241 tcacctgagc tttatattct ttcgccaacg cagcttgttt ttgttgctcg ctaagctgtt
     6301 tatcacgctc aagctgtgcc tttttatttt cttctaccgc cgcccgagct tcacgcgcct
     6361 gaacgcgcga ttttttcgcc gttctctcca ccttcgccgc ttttttgctg gtgactaatc
     6421 ctgctttgag caactgctct tgtaaggtaa gttttgccat ctttgtttcc aaacccgctg
     6481 aataattttt agcgagtata cctgtaaacg ccgtgggtgt attgggggca actccctttc
     6541 gcccctgctt aataaggcgc gtcaggcatc ggcacacggc tgcattcttg atctccgact
     6601 catcaaaata aacaccgcca acccagcaac aagaataccc ggtgcagaag cggccattac
     6661 gccgaccgtg ccagtgccta atgccagcat tttcccggcc agtaacggac cgctcatagc
     6721 ccccagacgc cctaccgcca cggctgttcc cacacctgtt gcgcggatct gcgaactgta
     6781 aaacaacggt gccagggcat acaaaacgct ttgcccacct gtcgcaaaca accccgcgac
     6841 aaatcccgcc agcaacatac cgttaaatga cgacaccgtt ccaagtgcca gcagcgaagc
     6901 taacatgccg ctataaatca gtagcgacat ggtcactgga cgcagcttat ccatcaatgc
     6961 gcccaacatt aacgtcccgc ttgccgcacc catttgcagg gcaaacatca cccctgccgc
     7021 ctgcgatggc tggaatcctt gctccaccaa aagcagcggt agccagttga tcaacatgta
     7081 gaccaccagc agagtgaaga aataacacaa ccacagcagc agcgtcgcgg ttgccgtttc
     7141 tggcgcaaat aaggcacgca gtggtggcgc agcctgttta tcgccagcga aaaccgccga
     7201 ctccggcagc cagcgcatta atagcggcac cagaatcaac ggcaccacac cacctaccca
     7261 aaacaccgtt tgccatgcta agtttgcccc cgcgaaaccc agtgtcgccg ccagcgccgc
     7321 gccaatggga acaccgcaat acatcaggct cactgctgtc ccacgaaaac gtggacccgc
     7381 ggcttcagac gtcagggcga taagattcgg caacgccgcc cccagcccga caccggtcat
     7441 cagccgcgca aagaccagtg aggggaaatc ccaggcaatc gccgttgcca gcgagaacaa
     7501 accaaacagc gcaactgagc caatcaaaat gcgcttgcga ccataacggt ccgccagcat
     7561 tccgccaacc aacgcgccgg gtagcaaacc gagtattccg gcgctgaata tccagcccat
     7621 ttgcatttta tcgagtgcga aagcctgggc gatgccaccc gccgcaatgc cagccgcctg
     7681 aagatccagc ccttccatca gagcgaccaa aaaacaaagc ccgatagtca gcatcaggcg
     7741 ggatgaagat gatgaagggg tacgagtcga cataagatga acctcgttgc gagtgcaggt
     7801 gaatcgcgac tgatacattg ccagccgcgt aatcagaact atttaatgac tgcctacggg
     7861 cgaaatgtag gccggacaag acgtttatcg catccggcag tcgtgtgcag atgcctgatg
     7921 cgacgctaac gcgtcttatc aggccaaatt ctcacgccac ccgtaggccg gataaggtgt
     7981 ttacgccgca tccggcagtc gtgctgggat gggcatacaa aatgcacgtt ctcggcagtc
     8041 atcattattt gttgttgcgc agatccagcg ccacgtcaac gatcatatcc tcctggccgc
     8101 caaccatccg gcgtttgccc agctcaacga gaatatccac cgcacttaag ccataacgcg
     8161 ccgccgccgt ttcacagtga cgcaggaagc tcgagtaaac accagcgtat cccagcgcca
     8221 gcgtttcgcg atcgactcgt accggtcgat cctgcaacgg acgcaccagg tcgtcagcgg
     8281 catccattaa cgcatagaga tcggtcccat gctgccagcc cagtttatcc gcggcggcaa
     8341 taaatacttc cagcggcgcg ttacctgcgc ccgcgcccat tcccgcgagg ctggcgtcga
     8401 ttcggtcgca gccctcttcc accgccgcga tagagttcgc cacgccaaga ctcaggttat
     8461 ggtgagcgtg catgccagtt tgcgtttctg gtttcagctc tgctttcagg gcgcggaaac
     8521 ggtcacggat atcgctcatg ttcatcgcac cgccagaatc caccacataa atacaggtcg
     8581 caccgtagcc ttccatcagc tttgcctgct ttgcgagatt ctccggcgtg gtcatatggc
     8641 tcatcatcag aaaaccaacg gtgtccattc cgagctcgcg ggcatactga atatgctggg
     8701 cggaaacatc agcttcggta cagtgcgttg ccacacgaac cacccgcgcg ccagcctgcc
     8761 aggcattttt cagatcgtga atagtgccga ttcctggcag caacaacgtc gcgattttgg
     8821 cgtgcttcac cacatccgcc gctgcttcaa tccattcaag gtcgctatgt gcgccgaaac
     8881 catagttaaa gctggaaccc tgcaaaccgt cgccatgggc cacttcaatc gaatccacgc
     8941 gggcatcgtc cagtgctttg gcaatctggc gaacgttttc cagcgaatac tgatgacgaa
     9001 tggcgtgcat accgtcacgc aacgtgacgt ccgagatata aagtttttta ccgttcatgc
     9061 cgcttctcct gccttgcgcg ccagtgactg ggccattttt tccgctgtcg ccagcgcact
     9121 ggaagtcata atgtcgaggt tgcccgcata ggcaggcaga taatgcgctg cgccttcgac
     9181 ttccagccag accgctgttt tcagtccgga gaattgcccc acgcccggta aattgaccgg
     9241 tttatcctgc gggataactt caaactgcac gcgctgtttc aggcgataac ccggtacgta
     9301 agcctgcacc gcctcagcca tttcattgat tgaggcttcg atatcatctt gtgaagcttc
     9361 gtcgctcaat acatacaccg tgtcacgcat catcaacggt ggctctgctg ggttaagcac
     9421 aataatcgcc ttcccttttg ccgcgccgcc caccacttca atggctcggg aagtggtttc
     9481 cgtaaattca tcgatattgg cacgcgtgcc aggtccggca gatttactgg cgatagaagc
     9541 gataatttcg gcgtaatgaa cacgcgccac gcgtgaaact gccgccacca ttggaatggt
     9601 ggcctggccg ccgcaggtga ccatgttgac gttcagttga tcgacgttcg cctcgaggtt
     9661 aaccaccggc acgcagtaag ggccgatggc agcaggcgtc aggtcaatta agcgaatatc
     9721 cggtttcgct tcgcgtaaag cggcatcgtt tttcacatgc gcgcccgcgc tggtcgcatc
     9781 aaatacaatg tcgatatcag caaattcagg catgttcatc agcccgatca ccccttcatg
     9841 ggtggtggcg acgcccatac gtctggcact cgccagaccg tcggactgag gatcaatgcc
     9901 gaccatcacc gccatctcca gatgctgacc gtgacgcaaa attttaatca tcagatcggt
     9961 gccaatgttg ccagaaccga taatggcgac tttacgctta ctcatgacag acttcctttt
    10021 ggggctgcgc ttgaaaatgt cgcagcaact gaacctatgc cttcaatatg ggcttcaaaa
    10081 cgatcgcccg cattcaccgc caccatcggg cctaatgccc cggtaagaat gatatctccg
    10141 gcgcgcagcg gttcacccag actggccatt ttgcgtgcca gccagacggc ggcattaagc
    10201 ggatgtccca ggcattcgct gccgcgcccg ctggaaacct cttcgttatt acgcgtcatc
    10261 ttcatggcgc agtttttcag gtctaacccc gccggacgtt gcgccggacc gccgatgaca
    10321 tacaccccgc aggaagcgtt atctgccacg gtatcgacaa actgaatcga ccagtcgcga
    10381 atgcggctcc ccaccacttc cagcgccgga agtacccatt caatggcgtt atacagttcg
    10441 tcgaaggtga tatcagttgc gggtaaatcg cggttcaaca ccagtgcgat ctccgcttca
    10501 atgcggggtt gcagaacacg ggaaaaagga atgatttcgt tatcgccata acacatgtcg
    10561 gcaaataacg tcccaaaatc cggttgatca acgcccagtt gttgttgcac tttcggatgc
    10621 gtcaggccca ctttacgccc taccacgcga cgcccctgca caacgtcata ttgcacattt
    10681 atgtgctgaa tggcgtaggc cgcttcagcg ttatcgatac caatcagatc gcgcagcggc
    10741 gcaatcgctt cgccctgctc tgcggcgcgg cgtaaatccg ccgccagttg ctcaagagta
    10801 tgcttcgtca tgaccattcc ttaagggcgt gcgaggaaat tcagcaccaa ttgattgaaa
    10861 gcgtcggcat gttcccactg cgcccagtga ccacagtcgc ggaagatatg cagttcagaa
    10921 ccggcaatgc cggacagcag acgcagaccc gcatccatcg gcacaaagcg gtcgttgcgc
    10981 ccccagacaa tcagggtttg cgctttgatt tccgccagac gtgggccaaa atccgggaac
    11041 tgtttcggat tagcttccag gctcttaacg aagttttcct ggtgatcgcg gcgcgacagc
    11101 atattattca ggcgcgcttc aaacagggcg tcggtcaaat cgctggtatc gaaaacgaag
    11161 atatccatca tcagcttcag gttttcgata gtcggctgac gataaagctg attcagtcgc
    11221 ttaatacctt cggttggcat cggcgtaaac aaactcatgc cgcccgtacc gccgcccatc
    11281 agcaccagtt tgccgacgcg ctccggccag ttaagggtga acgccacaga actatggccg
    11341 cccatcgagt tgcccagcag gtggattttg gcgatatcca gttgatccac cacgcttttc
    11401 aggattcgtg cattaagatc cgatcgtgaa ccactattaa cgatcgaatc gcttttgccc
    11461 caacccggac aatccagcag gattacccga tagcccgcct ctaccagcgg atcgatattg
    11521 cggctgaagt tcgcccagcc agtagcaccc gggccggaac catgcagcag gacaacggtt
    11581 tcgtcgcctt gtccgcagtc attaaaatgg atgcgcagcg ttttacccgc ttcttctaca
    11641 ttcagaaaac ggctggtggc ggcttcggtt tgtggctgat aactcatcat cttctcctgc
    11701 atattcagtt ctctgttctg gcgcttaacg agccaaatcc ggcaatccac tccgggattg
    11761 ggcggtaata acgcccttcg ctacgccagt tgccaaatgt agaaatagcg gcaaaagcgg
    11821 cgacccaggt tttgatttca tgtgtcgact ttccggcaat ggcggaaagc tcttcgttac
    11881 tgacggcatc cagttcctgt atgcgtccct gctccagcaa agtcatgaac tggttatccc
    11941 aaatcgggtt gagcggatgc agagttctct gatcctcaac aaacttctca gcggcgctaa
    12001 tcacccgttg ctgacgcaat tcgcgctcac tggcgggtaa atctttcccg ctccccaaca
    12061 gacggtcgcg catatgggca tcggctttcg ccagttcggg caccggcggc tgatgggaaa
    12121 gcccaccgga acccaggaac agcacgcgtt tattgagagt gctggtgaaa cgtccaatgg
    12181 cttcacccaa catgcgggta cgctggaaac cgggcagcgg cgtggcgaca ccgttgatga
    12241 acacaggcag aactggcacc ttatccagcc caccgagcag gaactccagc ggctgggcga
    12301 acccgtggtc cacctgcata cagtaagaaa cggcaagatc gatcccgctc ttcatgacag
    12361 catgcgcaca ggcctccgcc agctccacag gcacgggcag ctctcctgcc gcactgccga
    12421 aatcaccaat tgccgtcgct ccaacgccta aacagaacgg tggcatcacg tcatagaaaa
    12481 agccgttgta gtgatctggc gcaaacagca ccaccagttc aggggagaaa gccgcaatac
    12541 gctcgcgggc gctggcaatc acgccattga cctcatcgag tacctcttgc gccgggtcga
    12601 cataccccac caacggcgag tgggaaagac agtgaagata agcgtgcata tcaggctacc
    12661 ttttcgacag aaacgtcggc atcagggcgg gtcagcgtca tcaccgacgc cagtttattc
    12721 agcgtattgc ccagggtttg cggaatggcg gtggcggcaa caaagcgatc cgggcgcatc
    12781 accaccagcg aagcattgtg ttgcgcgaac cagctacgca gacgaccttg cgtatcgccc
    12841 acgcgtagca cgccgtcgtg gttatcctgt gcggtatgaa tttgcacttc cggcaccacc
    12901 tgaatgaagc gtgtgcccaa cgcgcgccac tgctggattt gctcgtcgct catcccccac
    12961 agtggattgc atccccagcc aattaccgcg aagttcgcgc caatcgcgtt atcgagcagc
    13021 gtcacgtcgc cgttttccag cgtgactttc ggctgaataa acatcttgcc gaccggagag
    13081 tgcttcgctt cgccctcacg taccagcgca ccgccgtaat attgcggcat cggcttgaag
    13141 cgcatttcga ggaagtagcg ttttactggc ggcagataat tcagtagcca ggaaacgccg
    13201 tcacgtaacg taccctgcca gcgtttcggc ggagccagca cgttgcccgc cgtcacggac
    13261 aggtcaatca tcgctttggc gtgatcgcga cgttcttgtt gataggtatc gagcagcaca
    13321 tcgcgggctt tcccctggat aaccaacgcc agtttccagg cgaggttaaa ggcgtcgcgc
    13381 ataccactgt tatagccctg cccctgccac accggcatga tgtgcgcggc atcgcccgcc
    13441 agcagtacgc gatcaatacg gaaacgttgc gccagtcgcg cgttatgggt gtagacacgc
    13501 tggcgaatca attcaacatt gtccggatta ggcagcactt tgcttaacag cttgcgcata
    13561 ttttgcggct cacgcagctg ctcttcggtt tctcccggca tcaccataaa ttcaaagcga
    13621 cgtaccgcat gaggcagcgc ggcagaaaca tacgggcgca ccggatcgca acacaaatag
    13681 atatgcggcg tacttaacgg atcgttggcg atatctacca caatccactg atttggcgca
    13741 gttttacctt caaacggcac attcagagtg cgacggacaa aacttgctcc accgtcacag
    13801 gctaccagcc actgggcttt gactatttcc cgctgccctt ctgccgtttt caggtgcaag
    13861 gtcacttcgt cattttgctg gctgaaggcc tccagctcgc gggaaaacaa gcagcgcaca
    13921 ttaggaaaac gcgacacccc ttccagcatc accgcatcga cctgcggctg aataaaggcg
    13981 ttacggcgcg gccagccaaa ttcatcggtc attggctgaa tatcagcaaa acagcggcct
    14041 ttcggggtga gaaaacgcat cgcgtgccac ggcgtagtgt gcggcagaac attctcgacc
    14101 aggccaaccg actgcatggt gcgcagcgtc tcgtcatcaa taccaatcgc acgcgggtag
    14161 tcgatcaact tatcgagttt ctccaccacc agcacgtcaa tgcccatctg accgagatag
    14221 ttcgccatca tcagcccgac cggaccggca cctgcgatcg ccacctgaac gctatggtta
    14281 acagcaggct ggatgtcagg gtgttgtatt gccatttcag tacctcacga ctcggacaaa
    14341 atgccgttgc gcgcacagta cggcgcaact tattttgtta aaaacatgta aatgattttt
    14401 tattgtgcgc tcagtatagg aagggtgttt tcggctacaa tcaaaacatg cccgaatgtg
    14461 caccaggtgc acaacgttgt tttaactata gaaatgtcaa ttaatatgca gaacaatgag
    14521 cagacggaat acaaaaccgt gcgcggctta acccgcggtc taatgttatt aaatatgtta
    14581 aataaacttg atggcggtgc cagcgtcggg ctgctggcgg aactcagcgg cctgcatcgc
    14641 accactgtgc ggcgactgct ggagacgctg caggaagagg gatatgtccg ccgtagcccc
    14701 tccgatgata gttttcgact gaccatcaaa gtgcggcaat taagcgaagg atttcgtgac
    14761 gaacagtgga tttctgcact ggcggcccca ctgctgggcg atctgttgcg cgaagtggta
    14821 tggccgacag atgtgtccac gctggatgtt gatgcaatgg tggtacgcga aaccactcac
    14881 cgtttcagcc gcttatcctt tcaccgggca atggtcgggc gacgtttgcc gcttctgaaa
    14941 accgcctcgg gcctgacctg gctggccttt tgcccggaac aagaccgcaa ggaattaatc
    15001 gaaatgttag cctcccgccc cggtgatgac tatcaactgg cacgggaacc gttaaagctg
    15061 gaagccattc tggcgcgcgc gcgcaaagag ggttacggac agaactaccg cggctgggat
    15121 caggaggaga agatcgcctc tatcgccgta ccgctgcgca gtgaacaacg ggtgattggc
    15181 tgtctgaatc tggtgtatat ggcgagcgca atgaccattg aacaggcagc ggaaaagcat
    15241 cttccggcgc tacaacgggt agcaaaacag atcgaagaag gggttgaatc gcaggctatt
    15301 ctggtggccg gacggcgaag cggcgtgcat ttacgttgac accatcgaat ggcgcaaaac
    15361 ctttcgcggt atggcatgat agcgttcgga agagagtcaa ttcagggtgg tgaatgtgaa
    15421 accagtaacg ctatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
    15481 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
    15541 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
    15601 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
    15661 gattaaatct cgcgctgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
    15721 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
    15781 gctcattaac tatccgctgg atgaccagga tgccactgct gtggaagcag cctgcgctaa
    15841 tgttccggcg ttatttcttg atgtctctga ccagactccc atcaacagta ttattttctc
    15901 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
    15961 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
    16021 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
    16081 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
    16141 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
    16201 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
    16261 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
    16321 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
    16381 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
    16441 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    16501 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    16561 ttccggctcg tatgttgtgt gaaattgtga gcggataaca atttcacaca ggaaacagct
    16621 atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    16681 ggcgttacac aacttaatcg ccttgcggca catccccctt tcgccagctg gcgtaatagc
    16741 gaagaagccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
    16801 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
    16861 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
    16921 tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg
    16981 acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
    17041 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg tcgctgggtc
    17101 ggttacggtc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc
    17161 ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat
    17221 caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact
    17281 acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta
    17341 ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct
    17401 ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc
    17461 gatgagcgtg gtagttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa
    17521 ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac
    17581 ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat
    17641 ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat
    17701 catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg
    17761 aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac
    17821 acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc
    17881 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc
    17941 gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg
    18001 aatgagtcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat
    18061 ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccttcgcaac cgatattatt
    18121 tgcccgatgt acgcgcgcgt ggatgaagac caacccttcc cggcggtgcc gaaatggtcc
    18181 atcaaaaaat ggctttcgct gcctggagaa ctgcgcccac tgatcctttg cgaatatgcc
    18241 cacgcaatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat
    18301 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat
    18361 gaaaacggca acccgtggtt ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc
    18421 cagttctgca tgaacggtct ggtctttgcc gaccgcacgc cgcatccggc gctgacggaa
    18481 gcaaaatacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc
    18541 agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat
    18601 ggcaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg
    18661 attgaactgc ctggactacc gcagccggag agcgccggac aactctggct tacggtacac
    18721 gtagtgcaac cgaacgcgac cgcatggtca gaagccggac acatcagcgc ctggcagcag
    18781 tggcgtctgg cggaaaacct cagcgtgaca ctccccgccg cgtcccacgc catcccgcat
    18841 ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac
    18901 cgtcagtcag gccttctttc acagatgtgg attggcgatg aaaaacaact gctgacgccg
    18961 ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc
    19021 cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa
    19081 gcagcgttgt tgcagtgctc ggcagataca cttgccgacg cggtgctgat tacgaccgct
    19141 cacgcgtggc agcatcaggg aaaaacctta tttatcagcc ggaaaaccta ccggattgat
    19201 ggtagtggtc aaatggcgat taccgttgat gttgaagtag cgagcgatac accgcatccg
    19261 gcacggattg gcctgacctg ccagctggcg caggtcgcag agcgggtaaa ctggctcgga
    19321 ttagggccgc aagaaaacta tcccgaccgc cttactgcgg cctgttttga ccgctgggat
    19381 ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc
    19441 gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc
    19501 agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa
    19561 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg
    19621 agcccgtcag tgtcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc
    19681 tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa
    19741 tccattatgt actatttaaa aaacacaaac ttttggatgt tcggtttatt ctttttcttt
    19801 tactttttta tcatgggagc ctacttcccg tttttcccga tttggctaca tgacatcaac
    19861 catatcagca aaagtgatac gggtattatt tttgccgcta tttctctgtt ctcgctatta
    19921 ttccaaccgc tgtttggtct gctttctgac aaactcgggc tgcgcaaata cctgctgtgg
    19981 attattaccg gcatgttagt gatgtttgcg ccgttcttta tttttatctt cgggccactg
    20041 ttacaataca acattttagt aggatcgatt gttggtggta tttatctagg cttttgtttt
    20101 aacgccggtg cgccagcagt agaggcattt attgagaaag tcagccgtcg cagtaatttc
    20161 gaatttggtc gcgcgcggat gtttggctgt gttggctggg cgttgtgtgc ctcgattgtc
    20221 ggcatcatgt tcaccatcaa taatcagttt gttttctggc tgggttctgg ctgtgcactc
    20281 atcctcgccg ttttactctt tttcgccaaa acggatgcgc cctcttccgc cacggttgcc
    20341 aatgcggtag gtgccaacca ttcggcattt agccttaagc tggcactgga actgttcaga
    20401 cagccaaaac tgtggttttt gtcactgtat gttattggcg tttcctgcac ctacgatgtt
    20461 tttgaccaac agtttgctaa tttctttact tcgttctttg ctaccggtga acagggtacg
    20521 cgggtatttg gctacgtaac gacaatgggc gaattactta acgcctcgat tatgttcttt
    20581 gcgccactga tcattaatcg catcggtggg aaaaacgccc tgctgctggc tggcactatt
    20641 atgtctgtac gtattattgg ctcatcgttc gccacctcag cgctggaagt ggttattctg
    20701 aaaacgctgc atatgtttga agtaccgttc ctgctggtgg gctgctttaa atatattacc
    20761 agccagtttg aagtgcgttt ttcagcgacg atttatctgg tctgtttctg cttctttaag
    20821 caactggcga tgatttttat gtctgtactg gcgggcaata tgtatgaaag catcggtttc
    20881 cagggcgctt atctggtgct gggtctggtg gcgctgggct tcaccttaat ttccgtgttc
    20941 acgcttagcg gccccggccc gctttccctg ctgcgtcgtc aggtgaatga agtcgcttaa
    21001 gcaattaatg tcggatgcgc cactgggcac atccgacaat tatttatagc tccatcatct
    21061 ggcgaaacaa gcgtcgcatc atacctcttt gacccacagc tgcggaaaac gtgctggtgc
    21121 aaaacgcagg gttatgatca tcagcccaac gacgcacagc gcatgaaacg cccagtccat
    21181 caggtaattg ccgctgatac tacgcagcac gccagaaaac cacggggcaa gcccggcgat
    21241 gataaaaccg atcccctgca taaacgccac cagcttgcca gcaatagccg gttgcacaga
    21301 gtgatcgagc gccagcagca aacagagcgg aaacgcgccg cccagaccta acccacacac
    21361 catcgcccac gataccggca attgcagcgg caaccagata aagccgcaga accccaccag
    21421 ttgtaacacc agcgccagca ttaacagttt gcgccgatcc tgatggcgag ccatagcagg
    21481 catcagcaaa gctcctgcgg cttgcccaag cgtcatcaat gccagtaagg aaccgctgta
    21541 ctgcgcgctg gcaccaatct caatatagaa agcgggtaac caggcaatca ggctggcgta
    21601 accgccgtta atcagaccga agtaaacacc cagcgtccac gcgcggggag tgaataccac
    21661 gcgaaccgga gtggttgttg tcttgtggga agaggcgacc tcgcgggcgc tttgccacca
    21721 ccaggcaaag agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt gataccaggt
    21781 ttcgctatgt tgaactaacc agggagttat ggcggcacca agtccaccgc cgcccatcag
    21841 agccgcagac cacagcccca tcaccagtgg cgtgcgttgc tgaaaccgcc gtttaatcac
    21901 tgaaggcatc accgcctgaa tgataccgat ccccacccca ccaagcagtg cgctgctaag
    21961 cagcagcaca ctttgggggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag
    22021 actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc
    22081 cagcccgccc atggtgacca ccggaagagc ggtcaacagg gcagccacgc taaagctcat
    22141 tccgctcgcc tggcgcaatt gcggtagcag tggcccgacg gaggtgagca gtggtcgcat
    22201 attaagacca atcagcacca gaaccagcag catcgtgaac gtcttttgat gattgcgcat
    22261 ggctgtcaga acggcttggt cggcagatat ttaccatcta aggtaatgac cgcacgttcg
    22321 ccaccttccg ggtccgccac tttcttaacg tcgagtttga agttaatcgc gctgataatg
    22381 ccatcgccaa acttctcatg aaccaacgct ttcagggttg tgccgtacac ctgcaacatt
    22441 tcatagaaac gatacatcgt tgggtcggtt ggaatacggt catcaatgca gccacgcagt
    22501 ggaatcatct gcaacagtag aatggcgtct tcgtcgagat ccagcttcgc cccgaccagg
    22561 cgggcggcgt cggcaggaag cgcttgctgc cccagcaaag ccgcggttac gaaggcttct
    22621 gccagaccgg taccgtcggc aatctcggca aatgagagat cttttttagc tttgctgagc
    22681 aaaatggcat cggcaagatc aagacgaata ttgcggttaa tttgtgactg aatcatggtg
    22741 gatctcctga tggtttaaaa ataaggttac gctgcggtcg gttggcgtag cggtatggca
    22801 caaacgcgag gattagcggc cagtggcaca aactggcggg ttgcgccgtc aaaagctgcg
    22861 atgctgccgc tttcaatgtc gtagacccag ccgtgcaggg cgatccgccc ctcttcgagc
    22921 gccaggcgca ccgatggatg agtttgcaaa ttagccaact gagcaatgac gttttcacgt
    22981 accatcgccg cagcttttga cggtaaatcg gaatgcgggc gcgcctcatt aacgacgcgg
    23041 gctgaatcgg cataacgcag ccagtgggag acggcaggca tatggtccat gcactgacag
    23101 ctggcaatgg cggtcatcgc gccacagttg gaatgaccac aaatcacaat gtcagatacc
    23161 cgaagcgcag cgacggcata ctccaccgaa gcagaaacgc caccgggttc cggcccgtag
    23221 gaagggacga tattgcccgc gttgcgaata acgaacagat cgccaggctc acgttgcgtc
    23281 accagctcag ggaccagacg gctgtcggag caggagataa aaagtgtgcg cgggctttgc
    23341 tgtgtcgcca gctgtttaaa caaggcttcc cgcttcggaa atgcctcgcg ctggaattta
    23401 aggaatccat caataatctc tttcaccgtt aacctctgtc tgtctctgga atgagaggca
    23461 gactactcgc cgattgtcat aaggtaaaag tctcatttat gatgagttct attggattta
    23521 cttataggtt ggcgaatgct ctctcgacat atcaattatt ttcttgccgt ggctgaacat
    23581 ggcagcttca cccgtgccgc cagtgcgttg cacgtctctc agcctgcgct ttcccagcag
    23641 attcgccagt tagaggagag tttaggcgtg ccgctgtttg accgtagcgg gcgaacgatt
    23701 cgtctcaccg atgcaggaga agtctggcga cagtacgcca gccgggcgtt acaggaactg
    23761 ggagcgggta aacgggcgat tcatgatgtt gccgatctga cgcgaggatc gctgcgtatc
    23821 gccgtcaccc ccacctttac aagctacttt atcggccctt taatggcgga tttctatgcg
    23881 cgctaccccg gcatcacgct ccagctacag gaaatgtcgc aggagaaaat cgaagatctg
    23941 ctttgccgcg acgagctgga cgttgggatt gccttcgcgc ctgtgcattc gccggagctg
    24001 gaggcaattc ctttactgac agaaagttta gcgttagtcg tggcgcaaca tcatccgctg
    24061 gctgcctgtg aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt cctgctcagc
    24121 gcggaatttg ccaccagaga gcaaattgac cactactgcg agaaagcggg gctacatcca
    24181 caggtggtca ttgaggcgaa ctcaattagc gcggttctgg agctgattcg ccgcacttcc
    24241 ctttccacat tgttaccagc agcgattgcc acacaacatg acgggcttaa agcgatttct
    24301 cttgccccgc cactactgga gagaacggcg gttttgttgc ggcggaaaaa tagctggcag
    24361 acagccgccg cgaaggcatt tttgcacatg gcgttggagg aatgtgcgga tgttggcgag
    24421 aatgaatcac ggtagccact gacacaggtc agtggctctt caacacagag gaaattaccg
    24481 tttgtaatcg atggcttctg gctgctccag atatacggtg gtttgtgccg gttgtgtact
    24541 ggcaatcacc ttgccgccac gtaccgaata acgtaccgga acctgacggc gcagcgcatc
    24601 aaatccattt tcagccggca ggataatcag gtttgcgctg tttccggcgg caatgccgta
    24661 atcctgcaaa ttcaatgttc tggcactgtg gtgggtgatt aaattcaggc catcgttaat
    24721 ctgcccgtag cccatcaact ggcaaacatg cagccccata tgcagcactt gcagcatatt
    24781 cgccgttccc agcggatacc acggatcgaa gacatcatcg tgaccaaagc agacgttaat
    24841 gccggactcc agcatctctt taacgcgcgt gatgccgcga cgttttggat acgtatcgaa
    24901 acgtccttgc agatgaatat tgaccagcgg gttggcgaca aagttaatac cggacatttt
    24961 cagcaagcgg aacaggcgtg aggtatacgc cccgttatag gagtgcattg ccgtggtgtg
    25021 gctggcggtg actcgcgcgc ccatgccttc acggtgcgcc agggcagcaa cggtttcgac
    25081 aaagcgcgac tgctcgtcat cgatctcatc acagtgaacg tcgatgagac ggtcgtattt
    25141 ttgcgccagg gcgaaggttt tatgcagcga ctccacgccg tattcacggg taaattcaaa
    25201 atgcggaatc gcccccacta catctgcccc taagcgtaac gcctcttcca gcaacgcttc
    25261 accgttggga tacgacaaaa tcccttcctg agggaaggcg acaatttgca gatcaatcca
    25321 cggcgcgact tcctgcttca cttccagcat tgctttcagc gcagttagcg ttgcatccga
    25381 aacatcgaca tgggtacgca catgctgaat gccgttggca atctgccatt tcagcgtttg
    25441 ccaggcgcgt tgtttcacat cgtcatgggt taataacgct ttgcgctcgg cccagcgttc
    25501 aatgccttca aacagcgtgc cggactgatt ccagttcggt tgtccggcgg tttgcgtggt
    25561 gtccaggtga atatgcggct ccacaaacgg cggtataact aaaccttgtt cggcatccag
    25621 gctgttttca gttatgggca tcacgccgga ttgcgcatca atggcgctga tttttccgtc
    25681 ctgcagatga atctgccaca gcacctcttt gcctggtaac cgggcgttaa taattgtttg
    25741 taaagcgtta ttcgacactg ttaacctcca catgcgtcat tgctgctgtc gttttacgat
    25801 tcaaaatcgg gttaaggatc agatagctca gcgcgccacc taataccgcg ttgaccggaa
    25861 caattcccgg taaccagtgg cctgcggcaa tccccagcgc gaccgccaga atcgccaccc
    25921 aattgacact catcatacgc gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat
    25981 agtcggcgat gatcacgccc cctactggag gaatagctgc cgaaaggaag gtcaaccagc
    26041 cgacaaaatt gttatacagc cataatgcgc acaccgtacc gataataccg ttgattaccg
    26101 aaagggtttt gctcgacata ccggtaatgt tggcgaaacc taaacccgac gcatagagtg
    26161 cgttatcgtt ggtggtccag atattcagcc ccagcaccac aatcgcaggc agcagcaggc
    26221 cctgagcaat catcacatca gagatatccg ccatgcccag tgccgcagcg cccgctgcac
    26281 cgaaaataaa catcaacgag ttgccgagga aaaaggccac catcgccacc agcaccgcca
    26341 gtttggcatt gcgaccaaac cggacaaagt cagcggtgag cgtacccgca ctgataaatg
    26401 accccacaac cagcgccagc gcgacattga aatctaacgg ttgtgcggga acgaccgctt
    26461 ttaatgcgtc caggccgccc atgccgttaa cagccagcca cacggaataa ccgcccaggc
    26521 aggcgatagc cggaaccgca atcaccgaaa gaaccgtcag cgccgaaatg ccaaaaaaga
    26581 cggtgacggt catcagtaaa ccggaaacgg caatcagcaa attaatatcc agcccggttg
    26641 ccttacccac cggaatggca aacatcgcca caccgacgcc aaaccagcca acctgagttc
    26701 cgcccagtag cagtgaaggc agccatgagc ctttaacacc aaacgagaag cgagcaagaa
    26761 gatgagtggt caggccggtt tttgcgccaa tgtaaccgag aaatgaagtg taaataccga
    26821 ggagaagatt accgatgaga actgcgagga agaaatcatg atagctaaga ccggttccga
    26881 gagtgccgcc ggtccacata ctggcggaaa agaaggttaa tcccagcatg acgaacgtca
    26941 atgccaatac ccctttccgc gccgactgcg ggactggccc ctggctaaag ttgttatctt
    27001 gcgacacgaa attccccctt tttgctgttt gaaacccaaa aaatccgccg tattctattc
    27061 atctgaagat aaaaagcaat cgttttcgtg gggaaatata ttttttatat gaatgagtgc
    27121 aaaaaggtgg gggaaaaatt atcggtgtaa aaacagtggg tagtgggtag atactgataa
    27181 gacgcgtcag cgtcgcatca ggcatcggcg cggggtgtcg gatgcgacgc gagcgcctta
    27241 tccgacctac ggttcaggcc tgtaggcata ataagacgcg gtaagcgtcg catcaggcat
    27301 cggcgcatgg tgtcggatgc gccacgcgac gcatccgaca ataccttact cttccatcgc
    27361 ctggcggatc tgatccaatg acgcaggatc atcaatggtc gtcagatccc caggatcgcg
    27421 tccttcgcaa attgcctgga tcgtgcggcg cagcattttt ccggatcgcg ttttcggcaa
    27481 ttgcgagaca aaccagacgt gcgccgggcg accaaagttg ccaatctggc tgtccaccag
    27541 cgccataatc gccttctctt gcgaatgcgc cacatcacga tcttccagac tatcgctctc
    27601 tttcggaatg acaaacgcca ccgccacctg ccctttcagc gcatctttca ccccaaccac
    27661 cgccacttcg gcaacgcccg gatggctgga gatactctct tcaatctcgc gcgtgcccag
    27721 acgatgcccg gcaacgttaa ttacatcgtc agtgcgcccg agaataaagt gataaccgtc
    27781 agcgtcacgg atgccccagt caaaggtggc gtacaccggg cgggaaaaca gcgaccagta
    27841 agtcttcaca aagcggtcgt cgtcgcccca gatggtctga atacagcccg gcggcagcgg
    27901 cccttccacc accagcatcc ctttctcgtt gacgccacac ggttcgccgg tgacttcatt
    27961 aagcaactgc acgttatagc catacatcgg cacgccgggg cttcccagac gcgtcggcct
    28021 gtcgtccaga ccgcgagcaa tcgccataat cggccagccg gattcggtct gccagtagtt
    28081 gtcgatgacc ggcacatcca gcgtattgct cacccaactg gcggtcggct cgtccagcgg
    28141 ttctccagcc agatagagca cttccagcga cgagagatcg tgtttgcgaa tttcagcggt
    28201 agggaatttt ttcagcacgc gaatggcggt cggcgctgag aacatccggc taacctgata
    28261 tttctcgacg attttccacc acacgccgca gtccggccag gtcggcaatc cttcgtaaac
    28321 gatagtcgcc atccccgcca gcagcggcgc gtaaacgata tacgaatgcc ccaccaccca
    28381 gccgatatcc gatgcgcaaa agaacacgct gcccgctttg ccgccaaaaa tggtgtccat
    28441 cgaggtcgcc agcgccaccg catatccgcc gacgtcacgc tgcacgcctt taggtttgcc
    28501 ggtcgtgccg gaagtgtaga gaatgcagga ggtttcgttg gattccagcc acgccaccgg
    28561 tacccgcgcg ccgatgtgtt gatggcgcaa cgacgcgaaa tcgacatccc gcccgctgac
    28621 gcgcgccatt ttcgccagcc cgcgatccac cagcaaaaca tggcgtggct ggtgctgcgc
    28681 ctgacttatc gcatcgtcga gcaatttttt atagggaatg attttgccac cgcgcgctcc
    28741 ggcatcagcc gagacaatca gcaccggttt agcgtcatca attcgcgccg ccacgctgtg
    28801 cgaggcaaaa ccaccaaata ccaccgaatg aatagcacca atgcgcgcgc aggccagcag
    28861 agtaatatgc gcttcggcaa tcatcggcat atacaccagc acccgatcgc cgcgctgcac
    28921 gcccagtgag cgcaacattg aggccaccgc gttcacttcg tcatgcagct ggcgaaaggt
    28981 aaaggtgcgc tcttcttctg tttccgaaga gacggcaatc aatgccagtg cctctggctg
    29041 tttctccagc cagcggtcga tagcgttgtg gcacaaattg gttcggcctt cacaaaacca
    29101 acgggcaaac ggcggattgc tgtggtcgag cgtttgcgta aagggcgtct gccagtcaat
    29161 acgccgggcc tgctcggccc agaactgctc cggttcgtta atcgaacgct gataaaattc
    29221 gctaaaagac ataatgctct cctgttgaac ttacgcctta ccgcagttta cttaaatgac
    29281 gtacaggtcg agatactcat tgaccggcat ctgttccagg cgagttctgt cgagagaaac
    29341 ctccagaatg cgctgctgtt ggcgagtcgg gaactggcgc gcgagattga ttttgaattt
    29401 atcgaccagt ttcggaatac catcctgacg gcggcgagca tgaccaatgg ggtactccac
    29461 caccacttct tcaaatcgtg tgccgtcggt gaactcaagg gtaatggcat tggcgatggc
    29521 gcgtttttcc gggtcgtggt agtcagcggt aaatgccgga tcttcaaagc aattgatctt
    29581 ctcgcgcagg gcgtcgatgc gtttatcttg cgcaacgttg tcctcgtaat ctgccgccgt
    29641 taagcgcccg aataacagcg ggatcgccac catgtactga atgcagtggt cgcggtcagc
    29701 cgggttattg agcggcccct ttttgtcgat gatgcgaata caggcttcgt gggtgcgaat
    29761 ggtcactttt tcgatatccg ccgccgtctt gcctgctgcc tgcatctgtt catagagcgt
    29821 catcgccgct tcaactgccg tctgggagtg gaactccgcc gggaaggaga ttttgaacag
    29881 cacattctcc atgacgtaag aaccgtacgg acgctggaag cggaacgatt cacctttaaa
    29941 ggagacgtcg tagaagcccc ataccggcgc ggtcagggct gacgggtaac ccatttcgcc
    30001 cgttttcgcc atcagtgcca gacgtaccgc gcgggaagtg gcatcgcccg ccgcccagga
    30061 tttacgcgtg ccggtgttcg gcgcatggcg ataggtgcgc agcgactgac cgtccaccca
    30121 cgccagcgaa acggcgttga gaatttcctc gcgggtcagg ccgagcattt cggcgaccac
    30181 ggcggtggaa gccactttca ctaacagaac gtggtcgagg ccgacgcggt taaaggagtt
    30241 ttccagcgcg atgcagccct gaatttcatg ggctttgatc attgcggtca gcacctgttt
    30301 catggtcaac ggcgctttgc cgctggcgac agcgttgcgc gaaagccagt ctgccgttgc
    30361 cagaatgccg ccgaggttgt cggaaggatg cccccactcc gccgccagcc aggtatcgtt
    30421 gaaatcgagc cagcggatca tcgcgccaat gttaaatgcc gcctggacag ggtcgagctg
    30481 aaactgagtt ccggggacgc gcacgccgtt gggtacgacg gtgccaggaa caattggccc
    30541 cagcagtttt ttacaggccg ggtattcgag agcttccaga ccgcagccga gcgtgtcgag
    30601 caggcagtaa tgtgcggtgt cgtaggccac tttagagctg atttcgtagt tcatcacgta
    30661 atcgacgata tcaacgattt cacgatcaaa ttccgggcgg atgttgttga tttgagctga
    30721 cattgggtat gtttccttat tgttattcgt agaggtttac tggcgcttat ccagcgcgac
    30781 aaactggcgg tcttccggtc caacataatt ggcggaagga cggataattt tgttgtcctg
    30841 acgttgttca atgatgtgcg ccgcccagcc ggtgacacgg gcgatgacaa acagtggtgt
    30901 gaacatctcg gtgggaacgc ccatcatgtt gtaggaaaca gcagagaacc agtcgagatt
    30961 ggggaacatc tttttgctct cccacatcac cgtttccagg cgattggcga tgttgtacat
    31021 cttcaacgag ccgccttcct gcgagagctg cttcgccaca cgtttgatca cctggtggcg
    31081 cgggtcggcg atggtataaa ccggatgacc aaaaccaatg accacttctt tgttttccac
    31141 ccgcttgcgg atatcggctt cggcttcgtc cggcgtttcg tagcgttgct ggatctccag
    31201 cgacacttca ttcgctccgc cgtgtttcgg cccgcgcagt gcgccaatcg cgccaataat
    31261 cgcggaatac atatcagagc cagtgcccgc aatcacccga ctggtaaagg tggaggcgtt
    31321 aaactcgtgt tcggcgtaca gcactagcga aatatgcatc gccttttccc agctttgcga
    31381 tggcttttcg ccgtgcagca gatgcaggaa gtgaccaccg atggagtcgt catcggtttc
    31441 cggctggatg cgttcgccgt tgtgactgta gtgataccaa taaaggagaa tcgagctaag
    31501 tgatgccagc agtttgtcgg caatatcccg cgcgccagag acagtgtgcc cctctttttc
    31561 tggcagtgtg cagccgagcg cggaaacgcc ggtgcgcatg acatccatcg ggtacgacgc
    31621 cgccggtaag gcttccagca cggtacgcac gttagccggt aaaccgcgca gggctttcag
    31681 tttcgttttg taagcggcga gttcgtcacg ggtcggcagt ttgccgtgga tcagcaggtg
    31741 cgccacttct tcaaattcgc aatgttccgc cagatcaaga atatcgtagc cacggtaatg
    31801 caggtcattg ccacttttac ccacggtgca gagcgccgta ttgcccgccg gaacgccaga
    31861 aagtgccaca gattttttcg gtttaatgac atgggtactg ttttgcagga tcgttgtgtc
    31921 gctcatgttg tcctcgtcat ttgtagggtc gagatatttt tcgttgtgtt ttatttcacc
    31981 tggctacggg caaacaggtc gtcgagcttc gcttcgtact ggtagtagtt gatgctttcg
    32041 tacagctcgt tgcgggtttg catggtatcg atgacgcttt tctgcgtgcc ttcctgacgc
    32101 aggacgttgt agacatgctc agcggcgcgg ttcatggcgc ggaacgctga aagcgggtac
    32161 agcgccattg cgacatgggc gctgcgtaat tcgtcgatgg taaacagcgg cgtggcacca
    32221 aattcggtaa tgttggcgag gattggcacc tgcaccgcat cggcaaactg gcggtacatg
    32281 gcgagttcgg taatcgcctc cgggaacaac atctcggcac ccgcttcaac ataggcctgc
    32341 gcacgctcga tcgccgcatc cagcccctct accgccagag catcggtgcg cgccatgatc
    32401 acaaaatcag gatcggtttt cgcatccacc gccgcgcgga tccgatccac catctcttct
    32461 ttcgagacga tcgctttatt cggacgatga ccgcagcgtt tcgcaccaac ctgatcttca
    32521 atatgcaatc ctgccgcacc ggctttaatc atcgatttca cggtgcgcgc cacgttaaag
    32581 gccgaagaac caaaaccgat atccgcatcc accagcagcg gcagcgaaca aacgtcggtg
    32641 atacggcgaa tatcggtcag cacgtcatca agagtagaaa taccgagatc gggcagcccc
    32701 agcgaacctg ccgccacgcc accgccagaa agataaattg cctgatatcc ggcacgctgc
    32761 gccaacagcg catgattagc gttgatggtg ccaacaatct gcaatggatt ttctttagtc
    32821 agtgcagcgc gaaacgcttt acctggagag tgtagagaca tagcccatcc tttgttatca
    32881 acttgttatt tgcgttgata aagacaaagc aaggggtgtg ccacaattgc cttaataaaa
    32941 ttaaatgttt aatttcaatt tgttagagat aaagtcatga actaatccgc aaatatgcgt
    33001 ttcagttaac gtttcaggca atgtttcacc gcgtttcatt gcaacaatta tgaaacaaga
    33061 ctaaacccaa tattcggttt cttaactttg cggtgcgcta tggcacatcc accacggctt
    33121 aatgacgaca aaccggttat ctggacggta tctgtaacgc gcctgttcga gctgtttcgc
    33181 gatatcagcc tcgagtttga tcacctggcg aacattaccc ctatccagct tggctttgaa
    33241 aaagcggtga cctacatcca caagaaactg gcaaacgaac gctgtgacgc catcatcgcg
    33301 gctggatcta acggcgcgta cctgaaaagc cgcctgtcag tgccagttat tttgattaaa
    33361 ccgagcggct acgatgtgtt acaggcactg gcaaaagccg gaaaactcac ctcttctatc
    33421 ggcgttgtca cttatcagga aactattccg gcactggtgg cgtttcaaaa aacctttaat
    33481 ttgcgcctcg atcaacgtag ctacattacc gaagaagacg cacgcgggca gattaacgag
    33541 ctaaaagcta acggcaccga agcggtggtc ggcgcggggc tgattaccga tctggcagaa
    33601 gaagccggaa tgaccggaat ttttatctat tccgccgcca ccgtgcgcca ggcgttcagc
    33661 gatgcgctgg atatgacgcg catgtcgtta cgccataaca ctcacgatgc cactcgcaac
    33721 gccctgcgta ctcgttacgt gctgggcgat atgctcggtc aatcaccaca gatggaacag
    33781 gtgcggcaga ctattttgct gtatgcccgc tccagtgcgg cggtgttgat tgagggggaa
    33841 acggggacgg gcaaagaact ggcagcccag gcaattcatc gggaatactt tgcccgccac
    33901 gatgcgcgac agggcaaaaa gtcgcatccg tttgttgccg tcaactgcgg ggcgattgcc
    33961 gaatcgctgc tggaagcgga gctgtttggc tatgaggaag gggcgtttac cggctcgcga
    34021 cgcggaggtc gcgccgggct gtttgaaatc gcccacggtg gcacgctgtt tttggatgag
    34081 attggcgaaa tgccgctacc tttgcagacc cggctgctgc gggtgctgga agaaaaagag
    34141 gttacccgcg tcggcgggca tcagcctgtt ccggtggatg tgcgggtcat tagcgccact
    34201 cactgcaagc tggaagaaga tatgcggcaa gggcagtttc gccgtgacct gttttatcgg
    34261 ctgagcattt tacgcctgca actgccacca ctgcgtgagc gggtggcgga tattctgccg
    34321 ctggcggaaa gctttttgaa agtgtctctg gcggcgctct ccgccccgtt ttctgccgca
    34381 ttacgcctgg gattacaggc aagcgaaacc gtgctggtgc actacgactg gccgggcaat
    34441 attcgtgaac tgcgcaatat gatggagcga ctggcgctat ttttaagtgt ggaaccgacg
    34501 ccggatttaa cgccgcaatt tttgcagctg ctactgccgg aactggcgcg cgagtcggcg
    34561 aaaactcccg ctccacgctt actgacacca caacaggcac tggagaaatt taatggcgat
    34621 aaaacagcag cggcgaatta tttaggcatc agccggacga cgttctggcg gcggctgaaa
    34681 agctgaactg ccgaatgcag cgcgaacgct ttatccggca taaatttcaa tagatgatca
    34741 ccgataaact tacgcatcgg gcatgtttac atagggttca gaattacttc ttcttataaa
    34801 tatttgccgt gccgtggatc ttattgtcag tttgaccgga ggtcagtacc aacacatcag
    34861 cccctttttc atccgctttt ttgatcaaat cttctttcgc gtctgcagtc gacatttcat
    34921 tgctagttga aatatcacct attttttcat actgcgattc aactttttca aattccgctt
    34981 tggtcatcaa ttcagcggca tagacattgg taacggctaa cgctaacgca ccgactaaca
    35041 ttttagagat tattttcata ttacggctcc agagttgtta tcacactatc atccacccac
    35101 attattgggt tgattaataa ttagttcagt cagggcataa acgccagtaa ccggaaataa
    35161 gacactttgt gatctctctc gcacccgatt atttttggta gaaatatgat gaagttactt
    35221 cacttattta tggatgaaat cactatggat cctttgcacg ccgtttacct gaccgtagga
    35281 ctgttcgtga ttactttttt taatccggga gccaatctct ttgtggtagt acaaaccagc
    35341 ctggcttccg gtcgacgcgc aggggtgctg accgggctgg gcgtggcgct gggcgatgca
    35401 ttttattccg ggttgggttt gtttggtctt gcaacgctaa ttacgcagtg tgaggagatt
    35461 ttttcgctta tcagaatcgt cggcggcgct tatctcttat ggtttgcgtg gtgcagcatg
    35521 cgccgccagt caacaccgca aatgagcaca ctacaacaac cgattagcgc cccctggtat
    35581 gtcttttttc gccgcggatt aattaccgat ctctctaacc cgcaaaccgt tttatttttt
    35641 atcagtattt tctcagtaac attaaatgcc gaaacaccaa catgggcacg tttaatggcc
    35701 tgggcgggga ttgtgctcgc atcaattatc tggcgagttt ttcttagtca ggcgttttct
    35761 ttgcccgctg tgcgtcgtgc ttatgggcgt atgcaacgcg ttgccagtcg ggttattggt
    35821 gcaattattg gtgtattcgc gctacgcctg atttacgaag gggtgacgca gcggtgagca
    35881 gataatttac tctctacgta atcaacctga tttgccgaat cccgaccatc ggcgatgatt
    35941 gcccccgtaa tgcgtggtcc ttatttccga taagtttact tgagtcttta gcttcccatt
    36001 tcagcccact ctcccctgtg tgggcttttt tcattggtct gacccgtcct gaatagccaa
    36061 acaatggcaa tgttccactt tgcagggatg ataataaggg gatctgattc agaagagttt
    36121 tgaaaagttg gaccgccatc gaggcctcga accccgtacc atacaacaat cctggttgtc
    36181 gtctgctctt cctgatgagc taatggcggt ttttgatatg agccttacag catattttct
    36241 gtcaggcttg agtgcgatgc taaggtacag aaaagacccc acacaaacat gatggaatta
    36301 tgattttttt catatgaatg tcaacccaca cattcacaaa acatctgcat gagatcattt
    36361 tttatacacc gcatagctgc gaaaccactg ctggtgcttt tttaagcgaa gcgtcgccga
    36421 tcacaacaaa atatcggcga aaaaggcggt ggaatgccat ttggcatacg gatatacaga
    36481 tgaaatgatt acctgctttg cgattcactc tctggtggaa taacaacttg caggtggaac
    36541 cctttctgaa ctaaagagtg tagcggattt taacgtgctt atttacagga taatatctgt
    36601 cacctaattc ctgcactcta ttcgatatcc attcatttaa tcgtgcgtca tctctgtatg
    36661 tttttacgtc cagaacagag agcaataaca tcaccagtga ttcaggaaaa acagggtgga
    36721 tattttttaa gtgtaataat aatccatgcg caagcgcctg cttaatgggc gcgtctgtct
    36781 ccggaataga cgacatttcg cgaagccatt tttccacacg catacactct ttccctttag
    36841 ggaaaattct cagcaaaggc atatggtgtt caagaagaat acatttgcaa tctgttcgca
    36901 ctgaatcgat aaactgcact aatttcttaa gcccggtaaa gcgtccagcc tgcacatttg
    36961 ctttaatatc aagaaacgca tcatcctggt catataccca aactgtccct ttttgatttt
    37021 caagtatgcg aaaaacaaac ttaaccgggt gatcagggta atccatccag tcataaaatc
    37081 cagggaaatc attaaactgc acatttatgg tgagtgcgtt atcgtctgac cactcacagt
    37141 ctcgtacggc agccatgaga tgaatgagtt tgtcgtcatc gactaaatca tcacgacaat
    37201 cataaggata atcataatat tgtttaatca aaaccttgaa tatatcgaca caggaaacca
    37261 gggagctttt gatatcctct ggtaactgat tgtttttaac cgtattaagt atgctttgtt
    37321 gcgtatatgg catcaaatta ttgtgcggag cttttaatga tatcatcgcg atctctctta
    37381 aataaaaata aacgtcgcag ataataaagc tctcattaaa ccaggcacta tcagaaatcg
    37441 ct
//
LOCUS       DAZFRJ010000023        36033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000023 DAZFRJ010000000
VERSION     DAZFRJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.023"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(181..1410)
                     /locus_tag="ACHKUN_001086"
     CDS             complement(181..1410)
                     /locus_tag="ACHKUN_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6659388.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNVLDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            1398..1580
                     /locus_tag="ACHKUN_001087"
     CDS             1398..1580
                     /locus_tag="ACHKUN_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659389.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            1587..2489
                     /gene="tqsA"
                     /locus_tag="ACHKUN_001088"
                     /pseudo
     CDS             1587..2489
                     /gene="tqsA"
                     /locus_tag="ACHKUN_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(2517..3905)
                     /gene="pntB"
                     /locus_tag="ACHKUN_001089"
     CDS             complement(2517..3905)
                     /gene="pntB"
                     /locus_tag="ACHKUN_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO6659390.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3916..5448)
                     /gene="pntA"
                     /locus_tag="ACHKUN_001090"
     CDS             complement(3916..5448)
                     /gene="pntA"
                     /locus_tag="ACHKUN_001090"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO6659391.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            5972..6916
                     /gene="ydgH"
                     /locus_tag="ACHKUN_001091"
     CDS             5972..6916
                     /gene="ydgH"
                     /locus_tag="ACHKUN_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO6659392.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7102..8484
                     /gene="ydgI"
                     /locus_tag="ACHKUN_001092"
     CDS             7102..8484
                     /gene="ydgI"
                     /locus_tag="ACHKUN_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6659393.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            8521..9243
                     /gene="folM"
                     /locus_tag="ACHKUN_001093"
     CDS             8521..9243
                     /gene="folM"
                     /locus_tag="ACHKUN_001093"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO6659394.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(9240..9575)
                     /gene="ydgC"
                     /locus_tag="ACHKUN_001094"
     CDS             complement(9240..9575)
                     /gene="ydgC"
                     /locus_tag="ACHKUN_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO6659395.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            9704..10423
                     /gene="rstA"
                     /locus_tag="ACHKUN_001095"
     CDS             9704..10423
                     /gene="rstA"
                     /locus_tag="ACHKUN_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO6659396.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10427..11728
                     /gene="rstB"
                     /locus_tag="ACHKUN_001096"
     CDS             10427..11728
                     /gene="rstB"
                     /locus_tag="ACHKUN_001096"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO6659397.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            11804..12733
                     /gene="tus"
                     /locus_tag="ACHKUN_001097"
     CDS             11804..12733
                     /gene="tus"
                     /locus_tag="ACHKUN_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO6659398.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12730..14133)
                     /gene="fumC"
                     /locus_tag="ACHKUN_001098"
     CDS             complement(12730..14133)
                     /gene="fumC"
                     /locus_tag="ACHKUN_001098"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO6659399.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(14276..15922)
                     /gene="fumB"
                     /locus_tag="ACHKUN_001099"
     CDS             complement(14276..15922)
                     /gene="fumB"
                     /locus_tag="ACHKUN_001099"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6659400.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            16121..17296
                     /gene="manA"
                     /locus_tag="ACHKUN_001100"
     CDS             16121..17296
                     /gene="manA"
                     /locus_tag="ACHKUN_001100"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO6659401.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSILQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            17397..18905
                     /gene="ydgA"
                     /locus_tag="ACHKUN_001101"
     CDS             17397..18905
                     /gene="ydgA"
                     /locus_tag="ACHKUN_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6659402.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(18950..20215)
                     /gene="uidC"
                     /locus_tag="ACHKUN_001102"
     CDS             complement(18950..20215)
                     /gene="uidC"
                     /locus_tag="ACHKUN_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO6659403.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(20254..21627)
                     /gene="uidB"
                     /locus_tag="ACHKUN_001103"
     CDS             complement(20254..21627)
                     /gene="uidB"
                     /locus_tag="ACHKUN_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO6659404.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(21624..23435)
                     /gene="uidA"
                     /locus_tag="ACHKUN_001104"
     CDS             complement(21624..23435)
                     /gene="uidA"
                     /locus_tag="ACHKUN_001104"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO6659405.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(23824..24414)
                     /gene="uidR"
                     /locus_tag="ACHKUN_001105"
     CDS             complement(23824..24414)
                     /gene="uidR"
                     /locus_tag="ACHKUN_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO6659406.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(24635..25402)
                     /gene="hdhA"
                     /locus_tag="ACHKUN_001106"
     CDS             complement(24635..25402)
                     /gene="hdhA"
                     /locus_tag="ACHKUN_001106"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO6659407.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(25514..26542)
                     /gene="malI"
                     /locus_tag="ACHKUN_001107"
     CDS             complement(25514..26542)
                     /gene="malI"
                     /locus_tag="ACHKUN_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO6659408.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            26717..28309
                     /gene="malX"
                     /locus_tag="ACHKUN_001108"
     CDS             26717..28309
                     /gene="malX"
                     /locus_tag="ACHKUN_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO6659409.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            28319..29491
                     /gene="malY"
                     /locus_tag="ACHKUN_001109"
     CDS             28319..29491
                     /gene="malY"
                     /locus_tag="ACHKUN_001109"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO6659410.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            29595..30596
                     /gene="add"
                     /locus_tag="ACHKUN_001110"
     CDS             29595..30596
                     /gene="add"
                     /locus_tag="ACHKUN_001110"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO6659411.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(30631..31671)
                     /gene="ydgJ"
                     /locus_tag="ACHKUN_001111"
     CDS             complement(30631..31671)
                     /gene="ydgJ"
                     /locus_tag="ACHKUN_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6659412.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            31914..32039
                     /gene="blr"
                     /locus_tag="ACHKUN_001112"
     CDS             31914..32039
                     /gene="blr"
                     /locus_tag="ACHKUN_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO6659413.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            32312..32527
                     /gene="ydgT"
                     /locus_tag="ACHKUN_001113"
     CDS             32312..32527
                     /gene="ydgT"
                     /locus_tag="ACHKUN_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO6659414.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            32613..33053
                     /gene="ydgK"
                     /locus_tag="ACHKUN_001114"
     CDS             32613..33053
                     /gene="ydgK"
                     /locus_tag="ACHKUN_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6659415.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            33130..33711
                     /gene="rsxA"
                     /locus_tag="ACHKUN_001115"
     CDS             33130..33711
                     /gene="rsxA"
                     /locus_tag="ACHKUN_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO6659416.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            33711..34289
                     /gene="rsxB"
                     /locus_tag="ACHKUN_001116"
     CDS             33711..34289
                     /gene="rsxB"
                     /locus_tag="ACHKUN_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO6659417.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            34282..>36033
                     /gene="rsxC"
                     /locus_tag="ACHKUN_001117"
     CDS             34282..>36033
                     /gene="rsxC"
                     /locus_tag="ACHKUN_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO6659418.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQV"
BASE COUNT         8736 a         9366 c         8778 g         9153 t
ORIGIN      
        1 gctatttaaa gagtgattcg ctagtgtata aaaaaccagt aacacattaa aaatcagcta
       61 cttataaatc tgtacacttt ttcgcctctt gttcgtagtt gttccgcatt gttgctcatt
      121 gttgcacgtt gtatctgttc gcatatccag tatgcgcata ctgaaaaaat acgaaaaaaa
      181 ttattttctt ctggctactg gtagcgtggt tacgttttca tgtgttcccg ccagtatccc
      241 taaccgctcc atccacatat ccagcacatt gcgtttagca tccagataac gggaatgatt
      301 ataaactcgc tgcattcctg gcatctggtg gcctgtaagc tgctccacga catgcggatc
      361 aacgcctaaa tcgttcagca tggttgtaaa ggtgcgccgg atgtcatgca gtgaccagtg
      421 aggatgatta agcctcctgt gcgctaatct tccatactgc gatacgctgg cctcctgttt
      481 cacttccccc agcaataagc ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg
      541 caggatcgct tccggtatgg gccggaatat tgcgaccttc gttttgctgt gctccttcgg
      601 aacggtccat agcatttccg taaaatccca ctccccgatc tccgatagcc tcagttctac
      661 cgtcctggct ccgaagacaa tcaggaggcg gattaacgcg acgtagtaag gggaaaatat
      721 ttttttgtcc agtgcctgca ataattcgcc aagttctttg ttacttaaga cacgttcgct
      781 tatatccggt tttttcccaa cgtccgccac gttcagatcg tccagaacgt tgctgattgc
      841 atagcgccgc ctacggcaga acttaagcgc ctgtttgcac gcctgtagca cgaatccggc
      901 agtaacaggc gttcgctttg ccacctggtc aaaacaggcc agccagtgcc gtagctcgca
      961 tttatccagc ggcatagcac caatctgctg tattacgtga ttattaagtc gccttttcag
     1021 ggcgatataa tccacgcggt tttcctttac gtaatactca agccagtagg tgagcgcatc
     1081 gccaaccgtt acgggcttta acgcttcctg tacggtgtaa ttcatctcat gacgtggatt
     1141 tttcccctca gccagccatg tgcgacactg tgcggctttt tccctggctg ctttcaggct
     1201 cagatcagga taacttccca gcttaatgcg ttcgggtcgt gtctcccttc ccgttccggc
     1261 cctgtatgtg aaataccagg tcaatttccc tgatgttaaa tatttcacgc tcaggtttcc
     1321 gccatcacta taaaacgtgt ttttctccgc tggcttacca tgaagtttcc ttagcaaggt
     1381 atcgctcagt ttgttcattg ctctgctcac gttttacccc ctctttacga aaaagtgact
     1441 acaccagtga ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa
     1501 ggaaaatcac aaatctgttg ataatcaaaa acataatgca cagcctgaaa cattatgaaa
     1561 cagcaaaaaa cactaaatga taaaatgctg ttattcttaa cccgctggtg caacacatgg
     1621 tccgctggcg agtgccgcgt gtactggcgg tgtcgatttt gatgaccatc atcgtgatgg
     1681 cgatggtgtt gctattagct tatctgggtt ccgcgctcaa cgagttgacg cggacgttac
     1741 cgcaatatcg caactctatt atgacgccgc tgcaagctct tgagccgttg ttgcaacgcg
     1801 tagggattga cgtctcagtt gaccagctgg cgcattacat tgatccgaac gcggcgatga
     1861 cgttgctcac caacttattg acgcagttat ctaatgccat gtcatcaata tttttattgc
     1921 tgctgacggt gctgtttatg ctgctcgaag tgccacaatt gcccggaaaa tttcagcaaa
     1981 tgatggcgcg tccggttgaa gggatggcgg cgattcaacg tgcaattgac agtgtttctc
     2041 attatctggt gctgaaaaca gccatcagca tcatcaccgg cctggtcgcc tgggcgatgc
     2101 tcgccgcact cgatgttcgc ttcgcttttg tctggggatt gctggccttt gcgcttaatt
     2161 acatccctaa tattggttca gttctcgcgg caatcccccc tatcgctcag gtactggtgt
     2221 ttaatggctt ctacgaagcg ttgctggtgc tggcgggata tctgctgatt aatctggtct
     2281 tcggcaatat tctggagccg cgcatcatgg gacgtgggct ggggctttcc acattggtgg
     2341 tatttttgtc gttgattttt tggggatggt tgttaggacc ggtgggtatg ctgctttccg
     2401 tgccgttaac aattattgtc aaaattgcgc ttgaacaaac agcgggaggt caaagcatcg
     2461 ccgttctgtt aagcgatctc aataaagagt ggcggcctcc gcagaggccg tcagggttac
     2521 agagctttca ggattgcatc cacgctggct ttggcgtcac caaacagcat gtgggtgttt
     2581 tccttgaaga acagcgggtt ttgcacacca gcatagccag tgttcatcga acgtttaaag
     2641 acaatcacgt tctgcgcttt ccacacttcc agcacaggca taccagcaat aggactcttc
     2701 ggatcatcct gcgccgccgg gttaaccgta tcgttagcac caatcaccag tacggtatcg
     2761 gtatcagcaa agtcatcgtt gatctcgtcc atttccagca cgatgtcata cggtactttt
     2821 gcttcagcca gcaatacgtt catatgtcca ggcaaacgcc ccgcaaccgg gtggataccg
     2881 aaacgcacgt tgataccacg agcgcgcagt ttctcggtaa tttcagcgac aggatattgc
     2941 gcctgcgcga ctgccatgcc gtaccccgga gtaatgatca ctgaatggga gtttttcagc
     3001 agttccgctg tctcttctgc ggtgatttcg cggtgctcgc ccacttcctg atcatcgcca
     3061 gtagaagagc cgtcggtgcc gaaaccaccc gcaataacgc tgataaagga acggttcatc
     3121 gccttacaca taatgtaaga aaggatagcg cccgaagaac cgaccagcgc accggtcaca
     3181 atcagcaggt cgttgctgag cataaagccc gcagccgcag ccgcccagcc ggagtacgag
     3241 ttcagcatcg acaccaccac tggcatatct gcaccaccga tggaggcgac taaatgccag
     3301 ccgaatacca gcgcaatggc ggtcattatc agcaatgcca gcacttgcag gccgacgcta
     3361 tccgtgcgaa caaatacaat cagcagcagg aaggaaacga ccagagccgc caggttcatt
     3421 ttgtgacggt ttggcagcat caacggttta gacgaaatct tgccacacag tttgccgaac
     3481 gccaccaccg aacccgtgaa cgttaccgcc ccgatgaaga taccgaggaa cacttccgtc
     3541 aggtgaatat tgaccagaat cggtgccatt cccgcgtcat gatgcagata actgttaaag
     3601 ccaaccagca ctgccgccag acccacgaag ctatgcagga tcgccaccag ttctggcatt
     3661 tcggtcattt caactttctt cgccagacgg ataccaattg ccccaccaat gaccatcgcc
     3721 agcaagatcc agccaacatt acccgtatcc ggtccaaaaa tggttgcgat taacgcaatc
     3781 gccatcccgg cgataccgaa gttgttaccc tggcgagacg tttcatgttt cgaaagaccg
     3841 gccagactga agataaacag gatcgcggca acaatgtatg cagctgtaac taatcctcca
     3901 gacatatgtt accccttaat ttttgcggaa cattttcagc atgcgctgag tcacggtgaa
     3961 gccaccgaaa atattaatgc tggctataag caccgcgata aaactaagga agctaaccca
     4021 gccgccctgg ccaatctgca acagtgctcc gacaacaata atccctgaaa tcgcgttggt
     4081 gaccgacatc aacggtgtat gcagcgcatg cgatacattc cacaccacgt aataaccgac
     4141 aacgcaggcc agcgcgaaaa cggtgaagtg cccaagaaac tctttcggcg caacgcttgc
     4201 cagccaaccg aaaaggatga ttgccagcgc catcaatgcg tatttacgcc acggtgagca
     4261 ggcacatttt tcctcagttt tcacttccgg tgccgctttt tgtgccgcct gcggctgagc
     4321 tgatacctga atcggcggtg ccggccaggt aatttcgccc gcacggatca cggtcacgcc
     4381 gcgaatcacc acatcatcaa aatcaacagt gatattgccg tctttctctt tgcacaacag
     4441 tttcagcaga ttaacgaggt tagtaccgta aagctgtgag gattgcgtcg gcagacggcc
     4501 cggaagatcg gtataaccaa tcactttgac accattttcc gtagtgaaga tttcacccgg
     4561 cacggtgtat tcacagttgc caccgttttg ggctgccagg tcgacaatca cactgcccgc
     4621 cttcatggag tcaaccattt cacgggtaat tagcttcggc gctggtttgc ctggaataag
     4681 cgcggtggtg acaatgatat cgacctcttt tgcctgggcg gcaaagagtt ccatttccgc
     4741 tttgatgaac gcgtccgaca tcactttggc atagccatcg ccgctgcccg cttcctcttt
     4801 aaaatccagc tcgaggaatt ccgcgcccat actttgaact tgttctttca cttccgggcg
     4861 ggtgtcgaat gcacgcacaa tcgcgccgag actgtttgct gcgccaatgg cggccagacc
     4921 tgcaacaccc gcaccaatca ccatcacttt tgccggtggc actttcccgg ccgcagtaat
     4981 ttgcccggta aagaagcgcc caaattcatg tgccgcttca acaatggcgc gataaccggc
     5041 gatgttcgcc atcgagctta gtgcgtccag cgattgtgcg cgtgagatac gcggcacaga
     5101 atccatcgcc atcacggtca cgttacgttc cgcaagtttt tgcattaatt ccggattctg
     5161 cgcaggccag ataaaactca ccagcgttgt cccaggattc agtaacgcaa tttcatcatc
     5221 taacggcgca ttgaccttca gaatgatctc tgactgccag acgctattcc cttctacaat
     5281 ttcagcgccc gcttgcacaa acgctttatc gtcaaaactt gccagttgac ccgcgccgct
     5341 ctctaccgcg acggtaaaac ccagtttcag cagctgttcc actgtttttg gcgttgctgc
     5401 aacacgggtt tcattggtta accgttctct tggtatgcca attcgcatga tattcccttc
     5461 catcggtttt attgatgatg gtttgcctgt gtcaggagcc acacaagccg ctcatgtacg
     5521 agctaaatgt tactccgtta aaataaatta gtaacaaacg ccctataacg tactgaaaat
     5581 tatgcctgtg atctagcgcc aaaaatcagt atttcggcgt gaactcgcaa aatattaacg
     5641 attcagccgt gatagtggga taaacacctt agaacgccgg ataaagactg ataattgtct
     5701 tcgacggtcg ggtaaaacga gacatcgccc cggcacgaat cactacttaa cattaaatta
     5761 acttatacaa ttcagttgct tcagtagtaa tgatgctgat acggctgttt tttaagcata
     5821 gacggtcatt tgagcaggat taaaattggc ttaaggaatg tgatatgaaa aatgacgcag
     5881 acagttacac cgtttaaatg caataatcag ccacgtttct cgttaataac aataccagta
     5941 cctggtttgc gcaaggcgaa ggattatttt tatgaagctt aagaacaccc tcctggcgtc
     6001 ggcactgctt tctgctacgg cattctccgt taacgcagca acagaactga caccggagca
     6061 agcggcagcg gttaaacctt ttgaccgtgt agtggttacc ggtcgtttta atgctattgg
     6121 cgaagcggtg aaagccgttt ctcgtcgcgc agataaagaa ggtgccgcct ctttttatgt
     6181 tgtcgacact tctgattttg gtaacagcgg taactggcgt gtggtcgctg acctctataa
     6241 agccgatgct gaaaaagcag aagaaacaag taatcgcgta attaacggtg ttgtcgaact
     6301 gccgaaagat caggctgttc tgattgaacc gtttgacacg gtcaccgtcc agggcttcta
     6361 tcgtagccag ccagaagtca atgatgccat caccaaagca gcaaaagcga aaggtgccta
     6421 ctctttctac attgttcgtc aaatcgatgc caaccagggc ggcaaccagc gtattactgc
     6481 attcatctat aaaaaagatg ctaagaaacg tatcgtccag agcccggatg tgatcccggc
     6541 agattccgaa gcaggacgtg cagctctggc tgccggtggc gaagccgcga agaaagttga
     6601 gatcccaggt gttgcgacta ccgcatcacc aagttctgaa gtcggtcgtt tctttgaaac
     6661 ccagtcatca aaaggcggac gttacactgt cacgcttcct gacggcacta aagtcgaaga
     6721 actgaacaaa gcgacagcag cgatgatggt cccgttcgac agcatcaaat tctctggcaa
     6781 ctacggcaac atgaccgaag tctcctatca ggttgcgaaa cgtgccgcga agaaaggtgc
     6841 taagtattac cacatcaccc gccagtggca ggaacgtggt aataacctga ccgtcagcgc
     6901 agatctgtat aaataactat tcagttactc ctataaggcg gcttgatgcc gcctttcgca
     6961 tttttagtca caaatttccc cgcgaccatt gcatgcagac acatctcttc gtaaaatccc
     7021 gcgcttcagt ggcaaactcc atttttatgc gttttgccct aatatttatt cttcttatca
     7081 cgttttaatc actggatatc gatggaaaag aaactgggac tgagcgcact caccgcgctg
     7141 gtattaagct caatgctggg cgcgggtgtt ttcagtctgc cgcaaaatat ggcggcagtt
     7201 gccagcccgg cagcactgct catcggctgg ggtattactg gcgctggcat tttattgctg
     7261 gcctttgcca tgctgatcct cacgcgcatt cgtcccgaac ttgacggcgg tatctttacc
     7321 tatgcccgcg aagggtttgg cgagctaatc ggcttttgtt ccgcatgggg atactggctg
     7381 tgcgcagtca tcgccaacgt ctcctatctg gtgatcgttt tttccgcgtt aagctttttt
     7441 acggacacgc cggaattgcg cctgttcggc gatggtaata cctggcaatc gatagtcggt
     7501 gcatcggctt tattgtggat tgttcacttt ttgatcctgc gcggggtgca aaccgctgcc
     7561 agcattaacc tggtggcgac actggcaaaa ttgttgccgc tgggtctgtt tgttgtgctg
     7621 gcgatgatga tgttcaaact ggataccttc aagctcgact tcaccggact tgcacttggc
     7681 gtacccgtgt gggaacaggt gaaaaacacc atgctgatca ccctgtgggt tttcattggt
     7741 gtggaaggtg ccgtggttgt ttctgcgcgt gcgcgtaata aacgtgatgt tggcaaagcg
     7801 acactgctgg cggttctctc cgctctgggc gtttacttgc tggtaacgct gctttcgctg
     7861 ggcgtggtgg cacgtcctga actggctgaa attcgtaacc cgtcaatggc cggtctgatg
     7921 gtggaaatga tgggaccatg gggcgaaatc atcatcgctg ccggtttgat cgtttccgtt
     7981 tgcggcgcgt acttaagctg gaccatcatg gcggcagaag ttccgttcct ggctgccact
     8041 cataaagcat tcccacgcat ttttgcgcgc cagaacgcac aagctgcgcc atccgcctcg
     8101 ctgtggttga ccaacatctg cgtgcaaatt tgtctggtac tcatctggct gaccggttcc
     8161 gattacaaca cgttgctgac catcgcctca gaaatgattc tggtgcccta tttcctggtc
     8221 ggcgcgttct tgctgaaaat cgcaacccgt ccactgcata aagcggtagg tgtcggtgcc
     8281 tgcatttatg gcttatggtt attgtatgcc tctggtccaa tgcacttgct gttatccgtt
     8341 gttctgtatg cgccggggct gctggttttc ctgtatgcgc gcaaaacgca tacacatgat
     8401 aatgtactga accgtcagga aatggtgctg attggcatgc tgttaattgc ctcagttccg
     8461 gcgacctgga tgctggtggg ataacgttca taccatcgtt ggatagcaaa ggagataacg
     8521 atgggtaaag cccagccctt gccaatatta attactggcg gaggtcgtcg catcggcctc
     8581 gccctcgcct ggcatttcat taatcaaaag caaccggtga ttgtcagcta tcggacgcac
     8641 tatccagcca ttgatggact gattaaggca ggtgcgcagt gtattcaggc tgatttttcg
     8701 accaacgacg gtgtgatggc gtttgccgat gaagtactaa aaagcaccca tggtctgcgt
     8761 gctattttgc ataacgccag tgcgtggatg gcggaaaaac caggtacacc actggccgac
     8821 gtactggctt gcatgatgca gatccacgtt aataccccat acctgctcaa ccatgcgctg
     8881 gaaagattac tgcgtgggca cggacacgcc gccagcgata tcattcactt taccgattat
     8941 gtggtggagc gcggtagcga caaacatgtt gcgtatgctg caagcaaagc ggcactggat
     9001 aatatgaccc gctcgtttgc ccgcaagctg gcaccggaag tgaaagtgaa ttctattgcg
     9061 ccatcgctga tcctgtttaa tgaacatgat gatgccgaat atcgacaaca ggcgctgaat
     9121 aaatcactga tgaaaaccgc gcctggcgag aaagaagtga tcgacctggt cgattactta
     9181 cttaccagct gctttgtcac cggacgcagt ttcccacttg atggcggtcg tcatctgcgt
     9241 taatgcagct ttatccagca tataatcaac acccatgcgc ttatccccca acacgcaaca
     9301 gaaccaacaa aagcggcggg caagcgcatc attccggtga aataccacag cgacaccagg
     9361 taaacaaaat agggaataat cgaccacata ctaaaaatga tggttgcgcg taaggcttca
     9421 atgccgcgtt cgctggcaac aatataatgc gcgataagcg caaaggtcgg gaaaagtgga
     9481 atcagcccgg cgatataata atttttcgtt tttgctaaaa caccaatcaa cagcactacc
     9541 agcgcaccga gcgcggcttt gattaccagc cccatctttt taccttaaca cttccataac
     9601 aagtcatcag tagaatacct gatgaaaact tgtttagaaa cgattgatag taagtaaaaa
     9661 cagcgcggtg tattgtgacg tttttatatc taccgtgaat gttatgaaca ctatcgtatt
     9721 tgtggaagat gatgcggaag tcggttcact gattgccgcg tacctggcaa aacatgatat
     9781 gcaggttacc gtagaaccgc gcggcgacca ggccgaagaa accattttgc gagaaagtcc
     9841 ggatttggtg ttactcgaca tcatgctacc aggcaaggac ggcatgacca tttgtcgtga
     9901 tttacgcgca aagtggtctg gaccgattgt tcttctaacc tctctcgata gcgatatgaa
     9961 ccacatcctg gcactggaaa tgggtgcctg cgactatatt ctcaaaacga cgccccctgc
    10021 tgttttgcta gcgcgtttac gtttgcattt gcgtcagaat gagcaagcca cactgaccaa
    10081 aggtcttcag gaaacgtctc tgactcccta caaagccctg catttcggca cgttgaccat
    10141 cgatcccatc aaccgcgtag tcaccctggc taacactgaa atctcgctct cgacagctga
    10201 tttcgaatta ttgtgggaat tagctaccca tgccgggcaa atcatggacc gcgatgcatt
    10261 gctgaaaaat ttacgcggcg tcagttatga cggactggat cgtagcgtgg acgtggctat
    10321 ttcgcggtta agaaaaaaac tgctcgataa cgccgcagaa ccttatcgca ttaaaactgt
    10381 gcgtaacaaa ggctatcttt ttgcgcctca tgcatgggaa taagcgatga aaaaactgtt
    10441 tatccagttt tacctgttat tgtttgtctg cttccttgtg atgtctctgc tggttgggtt
    10501 ggtgtacaaa tttaccgccg aacgcgcggg caaacagtcg ctggatgatt tgatgaacag
    10561 ttcgctgtat ctgatgcgca gcgaattgcg tgagatcccc ccacacgact ggggtaagac
    10621 gctgaaagag atggatttaa atctctcttt cgatctgcgt gtcgagccac tgagtaaata
    10681 ccatcttgat gatatttcca tgcaccgact gcgtggcggc gaaattgtcg ccctggacga
    10741 tcagtacacg tttttgcagc gtatcccgcg cagccactac gtgctggcag ttggtcctgt
    10801 tccttatctt tattatctcc atcagatgcg attgctggat atcgccctga tcgcttttat
    10861 tgctatttcc ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc aggatatgtt
    10921 aaaactggaa gcagcggcgc aacgatttgg cgatgggcat ctcaatgagc gtatccactt
    10981 tgatgagggt tcgagctttg aacgacttgg tatcgcattt aaccagatgg cggacaatat
    11041 caacgccttg attgccagca aaaaacagct tattgacggt atcgctcacg aactgcgaac
    11101 accgttagtg cgcctgcgtt atcgactgga gatgagcgat aacctgagcg ccgccgaatc
    11161 ccaggcgttg aatcgtgata tcagtcaact tgaagcttta attgaagagc tgctgactta
    11221 tgcccgactc gatcgcccac aaaacgagct tcatcttagc gaaccagacc tgccgttgtg
    11281 gctgtcaacg catctggcag atattcaggc agtaacgccc gataaaacgg tacggataaa
    11341 aacgctcgtg caaggccatt atgcggcgtt ggatatgcgc ttaatggagc gcgtgctgga
    11401 taatttgctc aataacgccc tgcggtactg ccattcaacg gttgaaacca gcctgctact
    11461 gtcggggaat agagcgacat taattgttga ggatgatggc ccagggattg ccccagaaaa
    11521 ccgcgaacat atctttgaac cttttgttcg cctcgatccc agccgggatc gctcaaccgg
    11581 cggctgcggg ctggggctgg caattgtcca ctctatagca ctggcaatgg gcggtacggt
    11641 taattgtgac accagcgaac tgggtggtgc tcgcttctcg tttagctggc cgttatggca
    11701 taacatcccg caatttacct ctgcctgaca ctacgcgcac gatggtcaag tcaccacgac
    11761 tgtgctataa aataagtatg ttgtaactaa agtggttaat attatggcgc gttacgatct
    11821 cgtagaccga ctcaacacta cctttcgcca gatggaacaa gagctggcta catttgccgc
    11881 tcatcttgag caacacaagc tattggttgc ccgcgtgttc tctttgccgg aggtaaaaaa
    11941 agaggatgag cataatccgc ttaatcgtat tgaggtaaaa caacatctcg gcaacgacgc
    12001 gcagtcgctg gcgttgcgtc atttccgcca tttatttatt caacaacagt ccgaaaatcg
    12061 cagcagcaag gccgctgtcc gtctgcctgg cgtgttgtgt taccaggtcg ataacctttc
    12121 gcaagcagcg ttggtcagtc atattcagca catcaataaa ctcaagacca cgttcgagca
    12181 tatcgtcacg gttgaatcag aactccccac cgcggcacgt tttgaatggg tgcatcgtca
    12241 tttgccgggg ctgatcaccc ttaatgctta ccgcacgctc accgttctgc acgaccccgc
    12301 cactttacgc tttggttggg ctaataaaca tatcattaag aatttgcatc gtgatgaagt
    12361 cctggcacag ctggaaaaaa gcctgaaatc accacgcagt gtcgcaccgt ggacgcgcga
    12421 ggagtggcaa agaaaactgg agcgagagta tcaggatatc gctgccctgc cacagaacgc
    12481 gaagttaaaa atcaaacgtc cggtgaaggt gcagccgatt gcccgcgtct ggtacaaagg
    12541 agatcaaaaa caagtccaac acgcctgccc tacaccactg attgcactga ttaatcggga
    12601 taatggcgcg ggcgtgccgg acgttggtga gttgttaaat tacgatgccg acaatgtgca
    12661 gcaccgttat aaaccacagg cgcagccgct tcgtttgatc attccacggc tgcacctgta
    12721 tgttgcagat taacgcccgg ctttcatact gccgaccatc tgttctggcc gtacccagct
    12781 gtcaaactcg gcttcgctaa gataccccag cgcaagggcc gcagctttta aggtcagccc
    12841 ttctttatgc gcttttttgg cgatctccgc ggctttgtca taaccaatgt gggtgttaag
    12901 cgcagtcacc agcatcagcg attcattgag taattgattg attcgctcac gattcggttc
    12961 aatacccact gcgcagtgtt tgttaaaact ttccatgcca tctgccagca agcgcaccga
    13021 ttgcaggaaa ttgtggatca ccattggacg gaagacgttc agttcaaagt taccggaagc
    13081 gccccccatg ttgatcgcca cgtcgttccc catcacctga cagcagagca tggttaatgc
    13141 ctcacactgt gttggattca ctttccccgg catgattgag ctgcccggct cattttccgg
    13201 gattgagatt tcaccaattc cgcagcgcgg gccagaggcc agccagcgga catcattggc
    13261 gattttcatc agtgacacag ccaacccttt caacgcgccg tgcgcctgaa ccagggcatc
    13321 acaggtcgcc agcgcttcaa atttgttcgg cgcggtaaca aacggtgcac aggtaatgac
    13381 tgccagttca tctgctacgc gacgcgcata ctccggatgg gtatttagtc cagtacccac
    13441 cgctgtaccg ccaagagcca gttccgctac gtgaggcagg ctgtattcga tatgtttgag
    13501 attatgctcg agcatcgcta cccagccgga aatctcctgc cctagtgtta gcggcgtggc
    13561 gtcctgcaag tgggttcgac cgattttgac gatatcggca aatgcacgcg atttttcact
    13621 cagtgtctgt gtcagggttt taagctgcgg aatgagttgc ttgcgcagcg ccagcagcgc
    13681 cgcaacgtgc atcgccgtcg gaaagacatc gttggaactt tggcttttgt tcacgtcgtc
    13741 gttagggtga actttacgtt ccatcccgcg cacgccgccg agtaattcac tggcccggtt
    13801 agccagcact tcgttcatgt tcatgttact ttgcgtgccg gagccggtct gccagatagc
    13861 cagcgggaat tcgtcgtcat gctgtcctgc cagtacttca tccgccgcct gccgaatggc
    13921 gctcgctttc tcttcagaca acaagcctaa atcttcatta acttttgccg ctgcgcgctt
    13981 ggttagcgcc agcgcatgaa tcagtgaggt gggcattttc tccgtcgaaa tgcggaaatg
    14041 ctccagcgag cgttgagttt gtgcgcccca cagcttatct gccgggacat caatcgcccc
    14101 catcgaatct ttttcgctgc gtactgtatt catggcctgc tcctcacctg tttaattttt
    14161 tctttctgtt ttgctttcgt taagcaactt ttagctcact tattatttac tatttgataa
    14221 caaatgtttg gtctttcgtg ccatgtaaaa aaaccgcccc gaagggcggc tctgtttatt
    14281 tcacacagcg agtgcactgt gtgagttgta tctgctggaa gaagtcattt cctttatcat
    14341 ccacaaggat aaacgccggg aaatcttcca cttcaatttt ccagatggct tccattccca
    14401 gttccggata ttcaacacat tccaggctct taatacttcc ctgcgccaat acagcggccg
    14461 gaccaccgat actgccaagg tagaagccgc cgtgtttttt acaggcatcc gtcacctgct
    14521 ggctgcggtt gcctttcgcc agcatgatca tacttccgcc ctgcgcttgc agttgatcga
    14581 cataagaatc catccgtccg gcggtcgttg ggccaagaga accggaggca taaccttccg
    14641 gcgttttggc cggacccgcg tagtaaatcg gatgatcttt aatgtactgc ggcagccctt
    14701 caccgttatc catccgctct ttcagtttgg cgtgagcaat atcacgaccg acgataatcg
    14761 tgccgttaag cgataagcgt gtagaaacgg gatactgcga caactgtgcg aggatctctt
    14821 tcatcggacg gttaaggtca acgcgcaccg cttcgccttc tcccgcttta cgcagctctt
    14881 cagggatata tttacctgga ttatgttcca gtttttcgat ccagatcccc tgacggttga
    14941 tcttcgcttt gatattacgg tcagcagagc aggaaacacc cataccaacc ggacaggatg
    15001 cgccgtgacg tggcaggcga atcacgcgta tgtcgtgagc gaagtattta ccaccaaact
    15061 gcgcacccag accgagattt tgcgcttcga tcagcaattc tttttccagt tccacatcgc
    15121 ggaatgcctg accgtgctca ttcccttccg ttggcagttc gtcatagtat ttagcggaag
    15181 ccagtttcac tgttttaagg ttagtttctg cagaagttcc accaattacg aacgcaatat
    15241 gatacggagg acaggccgcc gtacccagcg tgcgcatctt ctcaaccagg taatttttca
    15301 gtttccccgg cgttagtaac gctttggttt cctgatagag atacgtcttg tttgccgaac
    15361 caccaccttt ggcgatacag aggaatttgt actcgtcgcc atcaacggca taaagatcga
    15421 tctgcgctgg cagattggtg ccggtattca cctctttata catatccagc ggcgcgtttt
    15481 gcgagtagcg caaattatct tcgatataag tgttatagac accgcgcgcc agcgccgctt
    15541 catcacagcc accggtccat acacgctgcc cttttttacc aacaataatc gcagtgccgg
    15601 tatcctgaca ggttggcaga acgcctttcg ccgcgatgtc ggagttacgc aggaattgca
    15661 gcgccacata tttatcattt tcgctggcct ccgggtcacg cagaatgtcg gccacctgtt
    15721 gttggtgagc cggacgcagc ataaacgacg catcatgaaa cgcctgacgc gccaacagag
    15781 ttaacgcttc gggtgcgact ttcaaaatct cctgcccttc aaattcagat acgctaacgt
    15841 gttcgctggt tagcaggtaa tactcagtat catctttttt gagtggaaaa ggagcctgat
    15901 aatgaaaggg tttgtttgac attgttctct cacttactgc ctggtttggt tatgctctgg
    15961 gcgggtgttc cgttgccctg ttaaaagcga gtaacaatat cctacacact tttttaacaa
    16021 aaactgagac tagtacgact ttttgcggct ccaggttact tcccgtagga ttcttgcttt
    16081 aatagtggga ttaatttcca cattaaaaca gggattgatc atgcaaaaac tcattaactc
    16141 agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg gtatggaaaa
    16201 tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa gcagttcacg
    16261 agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga gtgataaatc
    16321 gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc tgttcaaagt
    16381 attatgcgca gcacagccgc tctccattca ggttcatcca aacaaacgca attctgaaat
    16441 cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc gtaactataa
    16501 agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg cgatgaacgc
    16561 gtttcgtgag ttttccgaga ttgtctccat actccagcca gtcgcaggtg cacatccggc
    16621 aattgctcac tttttacaac agcctaatgc cgaacgttta agcgaactgt tcgccagcct
    16681 gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat cggcgctcga
    16741 cagtcagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt acccggaaga
    16801 cagcggcctg ttctccccgc tgttactgaa tgtggtgaaa ttgaatcctg gcgaagcgat
    16861 gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg aagtgatggc
    16921 gaactccgat aacgtgctgc gtgcgggtct gacgcctaag tacattgata ttccggaact
    16981 ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc agccggtgaa
    17041 acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct cgctgcatga
    17101 ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt tctgcgtcga
    17161 aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg gtgaatcagc
    17221 gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt tagcgcgtgt
    17281 ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag cttattaaag
    17341 gcttataaca ccttcaggcg gccagtccgc ctgatttcat tttatggata atcattatga
    17401 ataaatcgct ggtagcggta ggcgtcattg ttgcgctagg cgtagtctgg acaggcggcg
    17461 catggtatac aggcaagaag attgaaaccc atctcgaaga catggtcgcg caggcgaacg
    17521 cgcaactcaa actgacagct cctgaatcca acctggaagt gagttatcaa aactatcatc
    17581 gcggcgtatt cagcagccag ttgcaactgt tggtgaaacc cattgccggg aaagaaaatc
    17641 cgtggattaa aagcggtcag agcgtcatct tcaacgaatc ggttgatcat ggtcccttcc
    17701 cgcttgccca gcttaaaaaa ctgaacctga tcccgtcgat ggcatcaatt caaaccacgc
    17761 tggttaataa cgaagtaagc aaaccactgt ttgatatggc aaaaggtgaa acgccttttg
    17821 agattaactc gcgcattggt tacagcggtg attccagttc cgatatttcg ctcaagccac
    17881 tgaattacga gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc cagttaaatg
    17941 ctgacagaga cggcaaagcc atctcccttt ccggggaggc gcaaagtggt cggatagacg
    18001 cagttaacga atacaaccag aaagtgcagt tgacctttaa taatctgaaa accgacggtt
    18061 ccagcacgct ggcaagtttt ggtgagcgcg taggaaacca aaaactgtca ctggaaaaaa
    18121 tgaccatttc agtggaaggc aaagaactgg cactgctgga aggcatggag atcagcggta
    18181 aatcggatct ggtcaatgac ggtaaaacga tcaatagcca actggattac tcgctaaaca
    18241 gcctgaaggt acagaatcag gatctgggca gcggcaagct gactttaaaa gtcggccaga
    18301 ttgatggtga agcctggcat cagtttagcc agcaatataa cgcgcaaact caggcgctgc
    18361 tggcacagcc agaaattgcc aacaatcccg aactttatca ggagaaagtg acggaagcct
    18421 tctttagcgc cctgccgctg atgttgaaag gcgatccggt gattactatc gcgccgctaa
    18481 gctggaaaaa cagtcagggt gaaagtgcgc tgaatctgtc gctgttcctg aaagatccgg
    18541 caacgactaa agaagcgccg caaacgctgg cgcaggaagt agatcgttcg gttaaatctc
    18601 tggatgcgaa actgaccatt ccggtggata tggcaactga gtttatgact caggtagcga
    18661 agctggaagg ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa gttgaaggtg
    18721 catcagcaat ggggcagatg ttccgtctga ccaccttgca ggacaatacc atcaccacca
    18781 gcctgcaata tactaacggt cagataacgt taaacgggca gaaaatgcca ctggaagatt
    18841 tcgttggtat gtttgcaatg ccggcattaa atgttccggt cgtacccgct attccgcagc
    18901 agtaattcac agacaaaacg ccggagattt ttctctccgg cgttattttt tacttcagca
    18961 taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg cccagcgtac
    19021 tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc ccttgtaacg
    19081 gcccttcgct gacccgatag ttcataccac ctaagatacc cgtgccttca atccgcacat
    19141 ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc gtaaattgcg
    19201 gcgttaaacg atagttgacg cccaattgcc aggactgcat tccttcacgg ttacgatcaa
    19261 gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc attgcatggc
    19321 gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc aattgtccac
    19381 taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg agctgcgcgt
    19441 aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg cttttctcga
    19501 gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga gaatagcgac
    19561 tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta tcgtattttc
    19621 ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca gtccataaaa
    19681 atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc caataaccta
    19741 atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt aacggaatac
    19801 gataaggtaa gctaccataa ctataatccg tcccgaagcg acctagttta agcaggctat
    19861 tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta tcaccatcaa
    19921 gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa tatgccccgc
    19981 cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat cctccctgta
    20041 cccatgcgta aatatctctt cccgcgccag cactgggctt atccgccctg cgcaattcgt
    20101 ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccgca taagcagagg
    20161 taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt ctcatagttg
    20221 cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc tgattaattg
    20281 ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt ttttatccgt
    20341 gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag gtactaaggc
    20401 aattgatgtg cggatgccca taataacttc cggcgtttgc acctgattgg cgatatatcc
    20461 gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc atttacgggt
    20521 aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt attcaccgta
    20581 ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct gaccaattga
    20641 agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc agaagaatag
    20701 cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt taccgatcct
    20761 cgcgaccatc cccggcacca gcggtgccga tgccacagta ccaaccaggt tttgcaccag
    20821 taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga acaacgacga
    20881 ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca acataaacag
    20941 cgggcgattc cgtttcaggg tttgcagact gatcttcaat gacggctgcg ccacgatacg
    21001 taccacattc tcacgcgtcg atttgaagca gatgaagtaa agcaccattc cggcaatcgc
    21061 cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg agttcttaat
    21121 gctcggtcct atcagaaatg ccaggcagac aaaggttaat gaagcggcaa tcccgcgagc
    21181 cgcgcccaga cgggcgcggg attgtggttg ttgggtcatc gcggtagcaa gtgaaccata
    21241 aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat atgcatacac
    21301 cactttgcta ccatggctcc agtcggtcgg cacccagaat accagcacgc tgaagatcat
    21361 taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg tattcacact
    21421 gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca ccagtaacag
    21481 catggtgccc gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac tcaataggaa
    21541 gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt agccgacgat
    21601 ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt ttcaccgaaa
    21661 ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt gcggtcgcga
    21721 gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt cgcaaaatcg
    21781 gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac gcggtgatac
    21841 atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat tgagtgcagc
    21901 ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag tttctcctgc
    21961 caggccagaa gttctttttc cagtaccttc tctgccgttt ccaaatcgcc gctttggaca
    22021 taccatccgt aataacggtt caggcacagc acatcaaaga gatcgctgat ggtatcggtg
    22081 tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc gagtttacgc
    22141 gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt atccggttcg
    22201 ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat cagctcttta
    22261 atcgcctgta agtgcgcttg ctgagtttcc ccgttgactg cctcttcgct gtacagttct
    22321 ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc gacagcagca
    22381 gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc ttcagcgtaa
    22441 gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc gtggtcgtgc
    22501 accatcagca cgttatcgaa tcctttgcca cgcaagtccg catcttcatg acgaccaaag
    22561 ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac tgccactgac
    22621 cggatgccga cgcgaagcgg gtagatatca cactctgtct ggcttttggc tgtgacgcac
    22681 agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac ttgcaaagtc
    22741 ccgctagtgc cttgtccagt tgcaaccacc tgttgctccg catcacgcag ttcaacgctg
    22801 acatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc ttgcgcgaca
    22861 tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag cattacgctg
    22921 cgatggatcc cggcatagtt aaagaaatca tggaagtaag actgcttttt cttgccgttt
    22981 tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt gttcacacaa
    23041 acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc ttcaaatggc
    23101 gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt gccgtaatga
    23161 gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg tataaagact
    23221 tcgcgctgat accagacgtt gcccgcataa ttacgaatat ctgcatcggc gaactgatcg
    23281 ttaaaactgc ctggcacagc aattgcccgg ctttcttgta acgcgctttc ccaccaacgc
    23341 tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc gagttttttg
    23401 atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt aagataaaaa
    23461 tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca gttatctgtg
    23521 agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca aaaaaccaaa
    23581 aactgcaata cccacagcgt gatattgatc gcattaatct ttaaaacggc gttgacagaa
    23641 gccataaaat aagcaaccga acgttcgttc gagtatattg ggaacgataa actctcatga
    23701 catatggcta cggtgaatat ttttgggggg gaattctccg atcccggcta gattgtattt
    23761 tgtacaggga aattaaagta tgaaacgcgg gcacagttat caccagcccg cgaaatgaga
    23821 ggatcaggat gcggttaaga taccgccaat catcgcgcgt aatccctgag caaacgacgc
    23881 ttcacgcgcc agatcttccg cttcgatatc ggccagtaca ccgtaggtca gatccagcag
    23941 taaacgtgaa gtcattgacg tgttgatgtc tgggcttatc tcgccttttt gctgggcatc
    24001 acgcatccgc tcggcaacaa attcagtgat cgtcatatgc ttatttttaa gcatggcggc
    24061 gacctgtggg ttacgcatcc cttccgccat aatttcgacc accagcgccc gttgcccaaa
    24121 ggcttcatgg gtgagagaga caatagactc gaccatatag tcaacaaaat gaatcccttc
    24181 aatcggttcc cggaaacggg ccagcgccct ctcctggtcc tgtaagataa tcgcctgaat
    24241 caaggcttct ttggagatga aatggtgata gagcgtcccg ggactaatgg cgcaggattt
    24301 acagatggct ttcatcgagg cactgtgaaa tccattttct gaaaaaatct ctctggcagc
    24361 attgaggatc cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca tcataaaacc
    24421 attatagtta gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct atcattgctg
    24481 ttgaatagtg cgaaggcaca ctctattcgt atacttttca ctctgaaatt ggttaaccaa
    24541 tagccgtgat ttggctgcca gttatttagc agagacgttg atgagtttat cccggtaaac
    24601 aacacgcacg gttgtttacc ggtccgttag tgtattaatt gagctcctgt accccaccac
    24661 cggagacggt gagaatttgt ccgcttaccc agctcgcagc aggcgagcaa aggaacagcg
    24721 ctgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc tgtaacattt
    24781 tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggcg
    24841 caatgccatt tacccgaata tttttttcac ccaggtcaaa cgccatattt ctgaccagat
    24901 gactggctgc agctttagat gatgcatagg aagtcatgtt tatattttta ttttctgccg
    24961 ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct ggcgcaacaa
    25021 gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga aaatccgcca
    25081 ttggcatatc aaacggttta ggtccaccgc caccggcgtt gttaaccaga atatcaactt
    25141 tacccagctt actgatagca aagtctgcca gtgcagagag ttcctgttcg gaagtaatat
    25201 cacaacggca ggcaaatgcc tgaccaccca gttgttgaat ttcgtctaca acatggttag
    25261 ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg
    25321 cgatttcttt accaatacct gcacccgcac ctgtgatgat ggcgcatttt ccgtcgagtc
    25381 tcaggttgtc agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac
    25441 cgcctgtgtg ccacattttt tatcattaac cttgaggaaa ggatactacc aggtatagca
    25501 gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat
    25561 gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtgata ccaagttccc
    25621 gcgctggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggt gtcgcatcgg
    25681 taaatgccgc cagcgaaacc tgttgctcaa agtaacgatc gactccgctt tccccgcttt
    25741 gccgccctgc tttgagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa
    25801 ccacggcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct
    25861 tctggctgga agtgcactcc aacacccaat cgctatgaaa cggcaggcca aattttagta
    25921 gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt tgccctccca
    25981 gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct
    26041 gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaaaa
    26101 tcaccgggat agctttttct tctgccatcc gtcgcaggtc atcgctgctt cctgcagccc
    26161 cggcaattac cacaccatcc acaccctgat tcagtaacag tgaaaaccgc tgtgccagct
    26221 gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct tccagagctt
    26281 ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac
    26341 caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc acaaatccca
    26401 gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccac
    26461 taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg
    26521 ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac
    26581 gttttacctt ttatttatct tatactcgct attatcgttg cgtaatgtga tttatgcctc
    26641 actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc
    26701 tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt gggagttctt
    26761 ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat
    26821 gctcggcatt ggtagttctc ttagtagcca cgatgtcatc accctgatcc cggtcctggg
    26881 caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag
    26941 tttcctgcca gtcatgttct gtatcgccat cccgctgggt ctggcacgcg aaaacaaagg
    27001 cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg
    27061 gttgaccaat aaaggcattc tgccaaccac ggacgccgcg gttctgaaag ccaataacat
    27121 ccagagcatt cttgggatcc agtcgatcga caccggtatc ctcggtgcgg tgatcgccgg
    27181 tattatcgtc tggatgctgc atgagcgatt ccataatatc cgcctgccgg atgcgcttgc
    27241 attcttcggc ggtacgcgct tcgtaccaat catctcctcg ctggtgatgg gccttgtcgg
    27301 cctggtgatt ccattagtct ggccgatttt cgcgatgggt attagcggct tgggccatat
    27361 gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt
    27421 gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg caggcggcac
    27481 gcaggaagtc tgcggtcaaa ccgtcagcgg cgcattgacc atcttccagg cgcaattgag
    27541 ttgcccgacc actcacggtt tttctgaaag cgccacgcgt ttcctttcgc aaggtaaaat
    27601 gcctgcgttt ctcggcggtc tgccaggtgc agcgttagct atgtatcact gcgcgcgccc
    27661 ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct gcgtcgttgg
    27721 cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat
    27781 ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg
    27841 taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac
    27901 caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt
    27961 ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag
    28021 ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttcctgcaat
    28081 cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct
    28141 gcgtttgtct gtgaaagata tgtcgcttgt taatgtgctg gcactgaagg acaatcgggc
    28201 aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca
    28261 gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat
    28321 gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt gggattatgt
    28381 cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc
    28441 cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tatttggcta
    28501 cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca
    28561 ttacaccgcc atcgattctc agacggtggt gtatggccct tctgtcatct atatggtttc
    28621 agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cacccgccta
    28681 tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga
    28741 gaagcaggct gatggttggt tttgcgatat gggcaagttg gaagccgtgt tggcgaaacc
    28801 agaatgtaaa attatgctcc tgtgtagccc acagaatcct accgggaaag tgtggacgtg
    28861 cgatgagctg gagatcatgg ctgacctatg cgagcgtcat ggtgtgcggg ttatttccga
    28921 tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga gtaatgtggc
    28981 gcggggagac tgggcgttgc tgacgtcggg ctcgaaaagt ttcaatattc ccgcgctgac
    29041 cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa
    29101 aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata tcgccgccta
    29161 tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta
    29221 tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc cgcaatccac
    29281 ttatctggcc tggcttgatc tacgtctgtt gaatattgac gacaacgcgt tgcaaaaagc
    29341 gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg aagaaggtcg
    29401 tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg
    29461 attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa aaaatcgttg
    29521 cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatattcaga
    29581 aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg
    29641 atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat atctcgcttc
    29701 ctgcacaatc cctggaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc
    29761 tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct cttgatgcct
    29821 gtcgccgcgt ggcatttgaa aacattgaag atgcagcccg tcacggcctg cactatgtcg
    29881 agctgcgttt ttcaccaggc tacatggcaa tggcacatca gctgcctgta gcgggtgttg
    29941 tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc
    30001 ttatcggcat tatgagccgg accttcggcg aagccgcctg tcagcaagag ctggaggcct
    30061 ttttagccca ccgtgaccag attaccgcac ttgatttagc cggtgatgaa cttggtttcc
    30121 cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg catattaccg
    30181 tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt gaactgggtg
    30241 cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg
    30301 ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtag
    30361 cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta
    30421 acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc gttgccgcgc
    30481 cagctgctgg gttatcccgc gagcaaatcc gccaggcaca gattaatggt ctggaaatgg
    30541 ctttcctcag cgctgaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaacaaa
    30601 atggatggtg caaattgcac catccatttt tcatgcaagg cacaaagtcg cgcgatgttt
    30661 ggcggattcg atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac
    30721 cggattttca ccatcgccat ttaacgcatc acgaatagcc gcatagtaag ccggataatt
    30781 cccaggtacc gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac
    30841 gccatcacgc atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg
    30901 ttcttcctgt ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata
    30961 ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata
    31021 ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc
    31081 taccgtcatg ctgaccggta aaccaaaaag cgtaatggcc tgatcaagaa gatgtggtgc
    31141 taaatcgtac cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg
    31201 cggacggaag cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc
    31261 gagtaaacct tttagcgtca agaaatcgct atcccagcga cggttatgga atacagacag
    31321 cacacgcccc aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt
    31381 aaagggttta tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa
    31441 atgggtatcg ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg
    31501 cttcggctca gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact
    31561 gctggagatt accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt
    31621 tttgctcgca taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc
    31681 tctcattgaa gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata
    31741 acctgccagc aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac
    31801 gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa
    31861 gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc
    31921 gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg
    31981 cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag
    32041 ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat
    32101 taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg
    32161 cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt
    32221 attgtaataa cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc
    32281 cctttggtat taattatttt atggaccttt tatgactgtt caggactact tattaaaatt
    32341 tcgcaaaatc agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct
    32401 gacggacgat caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct
    32461 ggtttctggc gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt
    32521 ccaataaaga aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat
    32581 ttgcctgttt gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg
    32641 gaggctggtt gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg
    32701 cgttgttgct gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac
    32761 aagcactgac gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt
    32821 attacacact gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact
    32881 atattatctg gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg
    32941 tggaagatgg catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg
    33001 tcccctattt caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt
    33061 acagttaacc tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca
    33121 ggccgaaaaa tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt
    33181 gtactggtca agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc
    33241 gcgatgggca tggggctggc aacaacgttt gtgatgacgc tggcgtctat ttgcgcctgg
    33301 cttatcgata cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt
    33361 attctggtga ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg
    33421 gtgctttacc gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc
    33481 ggcgtggcgt tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt
    33541 ttttccgccg ctatcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc
    33601 cttgctgtgg ctgatgtccc ggcacctttt cgcggtcatg ccattgcgtt aattaccgca
    33661 ggtcttatgt ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta
    33721 tctggattgc cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt
    33781 atgcctcccg ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct
    33841 taccgcagag ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca
    33901 tcagctgtaa cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa
    33961 aaattgccga gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagataa
    34021 cgcctgcgcg gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta
    34081 ttcaggcgtg tccggtagac gccatcgttg gcgctacccg tgccatgcat acggtaatga
    34141 gtgatctctg tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt
    34201 tgcaaccggt cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc
    34261 gtatcattcc cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc
    34321 tgggatttca acggcggcat ccatccaccg gagatgaaaa cccagtccaa cggtacaccc
    34381 ctgcgccagg taccactggc gcagcgtttt gttattccac tgaaacagca tattggcgct
    34441 gaaggtgagt tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt
    34501 ggtcgcggca aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg
    34561 ccccactcta cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat
    34621 ggtgaagact gctggatccc gcgcgacggc tgggccgatt atcgcactcg cagtcgcgaa
    34681 gagttaatcg agcgcataca tcagtttggt gttgccgggc tgggcggtgc aggattcccg
    34741 acaggcgtta aattgcaggg tggcggagat aagattgaaa cgttgattat caacgcggct
    34801 gagtgcgagc cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc
    34861 gtagagggta ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt
    34921 gaagataaca aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat
    34981 atttctctgc gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac
    35041 attctgaccg ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg
    35101 caaaacgtcg gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc
    35161 gagcgtgttg taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg
    35221 ctggggacgc cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa
    35281 atggtgatta tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc
    35341 gtaaagatta ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa
    35401 gaacaaagct gcatccggtg tagcgcctgt gctgacgcct gccctgcgga tcttttgccg
    35461 caacagttgt actggttcag caaaggtcag caacacgata aagctaccac gcataacatt
    35521 gctgattgca ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccctggtg
    35581 caatatttcc gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc
    35641 gcagaagcca aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcggct
    35701 cgccttgaac gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt
    35761 gctgccgctc tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt
    35821 aaagcgggcg aacgcccgga taacagtgca attattgcag cacgggaagc ccgtaaagcg
    35881 caagccagag cgaaacaggc agaactgcag caaactaacg acgcagcaac cgttgctgat
    35941 ccacgtaaaa ctgccgttga agcagctatc gcccgcgcca aagcgcgcaa gctggaacag
    36001 caacaggcta atgcggaacc agaagaacag gtc
//
LOCUS       DAZFRJ010000024        35837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000024 DAZFRJ010000000
VERSION     DAZFRJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.024"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(75..806)
                     /gene="rlmB"
                     /locus_tag="ACHKUN_001118"
     CDS             complement(75..806)
                     /gene="rlmB"
                     /locus_tag="ACHKUN_001118"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO6659419.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(986..3427)
                     /gene="rnr"
                     /locus_tag="ACHKUN_001119"
     CDS             complement(986..3427)
                     /gene="rnr"
                     /locus_tag="ACHKUN_001119"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO6659420.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRSGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3466..3891)
                     /gene="nsrR"
                     /locus_tag="ACHKUN_001120"
     CDS             complement(3466..3891)
                     /gene="nsrR"
                     /locus_tag="ACHKUN_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO6659421.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4096..5394)
                     /gene="purA"
                     /locus_tag="ACHKUN_001121"
     CDS             complement(4096..5394)
                     /gene="purA"
                     /locus_tag="ACHKUN_001121"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO6659422.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5498..5695)
                     /gene="yjeT"
                     /locus_tag="ACHKUN_001122"
     CDS             complement(5498..5695)
                     /gene="yjeT"
                     /locus_tag="ACHKUN_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO6659423.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5777..6781)
                     /gene="hflC"
                     /locus_tag="ACHKUN_001123"
     CDS             complement(5777..6781)
                     /gene="hflC"
                     /locus_tag="ACHKUN_001123"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO6659424.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6784..8043)
                     /gene="hflK"
                     /locus_tag="ACHKUN_001124"
     CDS             complement(6784..8043)
                     /gene="hflK"
                     /locus_tag="ACHKUN_001124"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO6659425.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8129..9409)
                     /gene="hflX"
                     /locus_tag="ACHKUN_001125"
     CDS             complement(8129..9409)
                     /gene="hflX"
                     /locus_tag="ACHKUN_001125"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO6659426.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9486..9794)
                     /gene="hfq"
                     /locus_tag="ACHKUN_001126"
     CDS             complement(9486..9794)
                     /gene="hfq"
                     /locus_tag="ACHKUN_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO6659427.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9880..10830)
                     /gene="miaA"
                     /locus_tag="ACHKUN_001127"
     CDS             complement(9880..10830)
                     /gene="miaA"
                     /locus_tag="ACHKUN_001127"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO6659428.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10823..12670)
                     /gene="mutL"
                     /locus_tag="ACHKUN_001128"
     CDS             complement(10823..12670)
                     /gene="mutL"
                     /locus_tag="ACHKUN_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO6659429.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12680..14017)
                     /gene="amiB"
                     /locus_tag="ACHKUN_001129"
     CDS             complement(12680..14017)
                     /gene="amiB"
                     /locus_tag="ACHKUN_001129"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO6659430.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14036..14497)
                     /gene="tsaE"
                     /locus_tag="ACHKUN_001130"
     CDS             complement(14036..14497)
                     /gene="tsaE"
                     /locus_tag="ACHKUN_001130"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO6659431.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14469..16016)
                     /gene="nnr"
                     /locus_tag="ACHKUN_001131"
     CDS             complement(14469..16016)
                     /gene="nnr"
                     /locus_tag="ACHKUN_001131"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO6659432.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16015..17154
                     /gene="queG"
                     /locus_tag="ACHKUN_001132"
     CDS             16015..17154
                     /gene="queG"
                     /locus_tag="ACHKUN_001132"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO6659433.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17137..17190)
                     /locus_tag="ACHKUN_001133"
     CDS             complement(17137..17190)
                     /locus_tag="ACHKUN_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659434.1"
                     /translation="MRFYFYSQAVDESGVTW"
     gene            complement(17424..17499)
                     /locus_tag="ACHKUN_001134"
     tRNA            complement(17424..17499)
                     /locus_tag="ACHKUN_001134"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17464..17466),aa:Gly,seq:gcc)
     gene            complement(17535..17610)
                     /locus_tag="ACHKUN_001135"
     tRNA            complement(17535..17610)
                     /locus_tag="ACHKUN_001135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17575..17577),aa:Gly,seq:gcc)
     gene            complement(17768..17843)
                     /locus_tag="ACHKUN_001136"
     tRNA            complement(17768..17843)
                     /locus_tag="ACHKUN_001136"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17808..17810),aa:Gly,seq:gcc)
     gene            complement(18054..18599)
                     /gene="orn"
                     /locus_tag="ACHKUN_001137"
     CDS             complement(18054..18599)
                     /gene="orn"
                     /locus_tag="ACHKUN_001137"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6659435.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18694..19746
                     /gene="rsgA"
                     /locus_tag="ACHKUN_001138"
     CDS             18694..19746
                     /gene="rsgA"
                     /locus_tag="ACHKUN_001138"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6659436.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19843..20811
                     /gene="asd"
                     /locus_tag="ACHKUN_001139"
     CDS             19843..20811
                     /gene="asd"
                     /locus_tag="ACHKUN_001139"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6659437.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20833..24156
                     /gene="mscM"
                     /locus_tag="ACHKUN_001140"
     CDS             20833..24156
                     /gene="mscM"
                     /locus_tag="ACHKUN_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6659438.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24185..24499)
                     /gene="yjeO"
                     /locus_tag="ACHKUN_001141"
     CDS             complement(24185..24499)
                     /gene="yjeO"
                     /locus_tag="ACHKUN_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO6659439.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24496..24810)
                     /gene="yjeN"
                     /locus_tag="ACHKUN_001142"
     CDS             complement(24496..24810)
                     /gene="yjeN"
                     /locus_tag="ACHKUN_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO6659440.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24862..26364)
                     /gene="yjeM"
                     /locus_tag="ACHKUN_001143"
     CDS             complement(24862..26364)
                     /gene="yjeM"
                     /locus_tag="ACHKUN_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6659441.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26583..27560)
                     /gene="epmA"
                     /locus_tag="ACHKUN_001144"
     CDS             complement(26583..27560)
                     /gene="epmA"
                     /locus_tag="ACHKUN_001144"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6659442.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27885..29693
                     /gene="frdA"
                     /locus_tag="ACHKUN_001145"
     CDS             27885..29693
                     /gene="frdA"
                     /locus_tag="ACHKUN_001145"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6659443.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29686..30420
                     /gene="frdB"
                     /locus_tag="ACHKUN_001146"
     CDS             29686..30420
                     /gene="frdB"
                     /locus_tag="ACHKUN_001146"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6659444.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30431..30826
                     /gene="frdC"
                     /locus_tag="ACHKUN_001147"
     CDS             30431..30826
                     /gene="frdC"
                     /locus_tag="ACHKUN_001147"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6659445.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30837..31196
                     /gene="frdD"
                     /locus_tag="ACHKUN_001148"
     CDS             30837..31196
                     /gene="frdD"
                     /locus_tag="ACHKUN_001148"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6659446.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            31259..32392
                     /gene="ampC"
                     /locus_tag="ACHKUN_001149"
     CDS             31259..32392
                     /gene="ampC"
                     /locus_tag="ACHKUN_001149"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6659447.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32481..33014
                     /gene="blc"
                     /locus_tag="ACHKUN_001150"
     CDS             32481..33014
                     /gene="blc"
                     /locus_tag="ACHKUN_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6659448.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(33011..33328)
                     /gene="sugE"
                     /locus_tag="ACHKUN_001151"
     CDS             complement(33011..33328)
                     /gene="sugE"
                     /locus_tag="ACHKUN_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6659449.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33510..33656)
                     /gene="ecnB"
                     /locus_tag="ACHKUN_001152"
     CDS             complement(33510..33656)
                     /gene="ecnB"
                     /locus_tag="ACHKUN_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6659450.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33767..33892)
                     /gene="ecnA"
                     /locus_tag="ACHKUN_001153"
     CDS             complement(33767..33892)
                     /gene="ecnA"
                     /locus_tag="ACHKUN_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6659451.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33944..34510)
                     /gene="efp"
                     /locus_tag="ACHKUN_001154"
     CDS             complement(33944..34510)
                     /gene="efp"
                     /locus_tag="ACHKUN_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6659452.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34552..35580
                     /gene="epmB"
                     /locus_tag="ACHKUN_001155"
     CDS             34552..35580
                     /gene="epmB"
                     /locus_tag="ACHKUN_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6659453.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8521 a         9857 c         9104 g         8355 t
ORIGIN      
        1 tacgtaatta tcttaccagc tatagacaaa aaaaaaccat ccaaatctgg atggcttttc
       61 ataattcaga cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca
      121 accgaaacgt tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa
      181 tgttcacggg tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaggcgt
      241 ccggtcattt tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag
      301 atattctctt cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga
      361 acgctttctg ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga
      421 tctttcggca caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg
      481 aggttgtgcg gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg
      541 atcagatccg gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg
      601 ccctgatgca cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata
      661 accacgccct gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct
      721 tttaaaataa agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg
      781 tggatgccgt aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca
      841 ctacgtttat ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc
      901 cggataaggc gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac
      961 cctcttcttt taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg
     1021 ctttggtcgc tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt
     1081 ctttcttcgc cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa
     1141 agtttacctt tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt
     1201 tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac
     1261 taaagtcgat tttgcgctcg tccatattaa ccgcttcgac tcgaacttcc acgcgatcgc
     1321 ccaggcgata agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc
     1381 gatagtagtc attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca
     1441 gacggacgaa gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta
     1501 cctggtcgag catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg
     1561 cacgacgttc cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat
     1621 ggtagccgcc ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatatt
     1681 taatggcgcg gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat
     1741 aggactgcaa tgccagaccg aagtgaccac ggttttctgg atcgtaaatc gcctgtttca
     1801 tcgagcgtag cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca
     1861 gcagttccgc gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct
     1921 ccgccagcac tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga
     1981 acagtgccgg ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca
     2041 tgcactcttc aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac
     2101 ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt
     2161 cttcacgggc tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca
     2221 gcggggcgta ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt
     2281 aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc
     2341 ctttcgacga aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg
     2401 aacacaggcc gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca
     2461 ccgacgtgcc acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc
     2521 tgacgtcggc aatcgcgacc cataaacgcc agccgccgct gcgttttttc tcgcagtaaa
     2581 ctgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca
     2641 gatcaacacg gcccgctttt gcttcttccg gcacttcttc tttcagcccg gcaacctgtt
     2701 gctcaacagc ctgcggccag atgtacggaa tttcatgggt acgcagagcg atatcaaccg
     2761 ccatgccggt gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc
     2821 ggcgagtcgg acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga
     2881 tctgatcggg cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc
     2941 cgacgcccgc ttcagtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc
     3001 ggacaatacg cgcttcacga cgacctttac ggtcagcgcc cagcggctgc gccagcacct
     3061 gatcgccatg aatgcaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc
     3121 cttcaacccg cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt
     3181 cgaggcgttc cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct
     3241 ccatcgcgcg caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt
     3301 ctaccgccag ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa
     3361 attcccgact agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt
     3421 gtgacatctc ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa
     3481 taatttataa agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc
     3541 cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat
     3601 gtggcaaaac tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc
     3661 accaatacgt atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac
     3721 agcagtcacg tagccggcac gactaagttg attgattatt ttgaccatat gattacggga
     3781 gacgccgtag acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc
     3841 catgtagatc agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc
     3901 aaaagggaaa tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc
     3961 gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc
     4021 gtaatggctt aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg
     4081 ccaggcgtac cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc
     4141 cggaccggta gagatgatat cgatcggcac accagtcagc tcttcaatac gcttgatgta
     4201 gttcagcgcc gcctgcggca gaccgctacg atctttcacg ccgaaggtgg attcagacca
     4261 gcccggcatg gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt
     4321 ggtcgtcact tcacgaccat ccggcatacg gtaagccaca cagagtttca cctctttcag
     4381 gccatccaga acgtccagtt tagtcaggca gaagccagac agggagttca gctgtaccgc
     4441 acgacgaacg gcaacggtgt ccagccagcc ggtacgacga cgacgccccg tagttgcgcc
     4501 gaattcgtta ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa
     4561 cggacctgca cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata
     4621 acgcgggccc aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac
     4681 gtacggataa gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac
     4741 gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt
     4801 caggatgtcg gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc
     4861 tttgtagtag ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc
     4921 gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc
     4981 ataagcaggc ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc
     5041 acgcgcgtta tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag
     5101 cagacgctca cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc
     5161 ggccggagac agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc
     5221 tgatggaata agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt
     5281 gtgaccgccc tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt
     5341 acctttacct tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca
     5401 aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt
     5461 tgcactcaaa aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca
     5521 tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat
     5581 cgggcaaatt ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc
     5641 ctaaaccttc cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt
     5701 atccttatag aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg
     5761 taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat
     5821 cgctatccgg gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac
     5881 gcaggctacg gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt
     5941 tggctgcttc ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca
     6001 gcgttctggt cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt
     6061 gtgaacggtg acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt
     6121 cagacacttc ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca
     6181 gcgccgccat actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc
     6241 gctctgccgc ttcggcaatg gcgttatctg ctgccggggt agtaacttca tcttctgtac
     6301 ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcag
     6361 tgacgatatc tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac
     6421 gtttcaacag cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc
     6481 tgaaatcgct gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg
     6541 tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg
     6601 tttcaatgaa cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat
     6661 cgtcacgcag taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa
     6721 agacagacat gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca
     6781 tcgttattcc ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat
     6841 aatatcgccc tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc
     6901 tggcggcaga cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc
     6961 acctttcagc atctggtcta acggcagaac catcaggttt ccacctttgt cgttaaccag
     7021 cactttgcgg gtgttgccca acactttttc catcgtctcg atatacagac gctcgcgagt
     7081 aatttccggc gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg
     7141 agcttccagg atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg
     7201 accgttcgca cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg
     7261 ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg
     7321 agcagcctgg aagttgacgt ccagcagcgt gatacccatg tcatacggac gaatcgtctc
     7381 ttccagttcg cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc
     7441 catggtgtat ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc
     7501 atccgggctg gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt
     7561 catctcaacg cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc
     7621 acgcacggct tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag
     7681 acccggctca accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc
     7741 tttaatggta tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt
     7801 aacgacacga ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc
     7861 ggtgccttta ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc
     7921 taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc
     7981 aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc
     8041 catgtttgtg ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat
     8101 atgactccac gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt
     8161 gtttacagag gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt
     8221 cctcctccat ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgcc
     8281 cttcctgcgg tggcagacgc aatgtatgct gcgccacctc gccggaaagc cgccccgtca
     8341 aagcctgaaa aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg
     8401 gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt
     8461 tcatcaccag cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg
     8521 cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc agtaatgtgg
     8581 cttgccgcgt ctcttgtaac gtggctttaa atgccgccac cagatcgtgc ggcaggtggc
     8641 gaataaaccc tacggtatct gcaagtacgg tttcaccgac atctgcaacg tcaatacgcc
     8701 gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga
     8761 tgcgattgaa aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa
     8821 cgtcggcttt gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttccaggc
     8881 gtgactgtat ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt
     8941 cacccggacc acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca
     9001 ccaggcgcgt agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg
     9061 tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact
     9121 cgcacaaacg ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga
     9181 ccgaagcacc cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa
     9241 aatactttgg gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg
     9301 cggaagagac cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat
     9361 agatgtgtac cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc
     9421 tctaaataga tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg
     9481 aaaccttatt cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca
     9541 tgatggtagt tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga
     9601 gacgggacaa cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac
     9661 aggatcacga attgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa
     9721 taaatagaaa ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat
     9781 tgccccttag ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt
     9841 ctgaaaaaat tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac
     9901 aacctgtaat acttcgtcgc gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc
     9961 ttcccaacca cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc
    10021 tcgataaacc atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata
    10081 acccacgcaa cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg
    10141 gacttctgct tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg
    10201 gagcagttca cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc
    10261 tcctgacgtt tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc
    10321 ccgggaaagc ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc
    10381 ctgaagttga cgatgcaatg actcccaacc ttgctctacc gcctgttgct caattctggc
    10441 ccgcacttcc gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata
    10501 caacatcgta ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc
    10561 cgccagcgca tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc
    10621 cagcaatcgg tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc
    10681 catccctttg taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg
    10741 cagctcaatg gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt
    10801 aggcaggctc gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat
    10861 caacagattg taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca
    10921 cgtccgccag cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat
    10981 ttcgtgcaat ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc
    11041 ctatcagttc aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc
    11101 tgatggtcac atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact
    11161 gcgctttttc taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaatcagca
    11221 acggctgggc gcaaactggc gcttcacccg gcgtcagttg cgcctgacgc agccaacgtt
    11281 ctgccactgg caaggctaaa agtgaaatgt tgccatcgcg ctccagcaac gcacagtcgg
    11341 aatggacgat agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct
    11401 gcggttccgg cgcttttggt ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca
    11461 cttcaccttg ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac
    11521 tgcctgaggc gggcgcagga gagtagcgcg gagctaccgg ctcacgagct gccggttctg
    11581 caaagtgatt gcgccccgcc gccacgcggt tttccggaat gggacgcggt gcaggttggg
    11641 gttcatcgtc cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct
    11701 gatagataaa atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt
    11761 gtacgttgac gtccacctga tgcgggtcga tctccagata caacacaaac gccggttgtt
    11821 gatcggcccc cagtttgtct tcgcaggcct ggcggatcgc atgattgatc aggcgatcgc
    11881 gcatcatgcg accattcacg tagcaatact gaatttccgc cagtgcgggc gtagtgtgat
    11941 ttggatcggc cacccagccg cgcagcgtga gatcgccgtg ttgccattca atcgccagcg
    12001 cttgttcgag aaaagcggtg ccacaaatcg cgcctaagcg ccgttctttt tgcccgcctt
    12061 ccggcactgc gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt
    12121 cgaaacgcgc cagcgcaatg cggcggatga tttcatcaat atggctaaat tcggttttct
    12181 cggtgcgcag gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg
    12241 tcgtccccac aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat
    12301 aggcctgcca ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg
    12361 aactgatact cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt
    12421 ccagagaggc gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt
    12481 tgataccgca gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa
    12541 tatcgatacg cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg
    12601 caggtcgctc gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct
    12661 gaattggcat caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg
    12721 cagtttgtgc cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc
    12781 gcaggccttt gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac
    12841 gttcttcgct gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg
    12901 aacgcagaac gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt
    12961 gcaactgact gatcatactg gtcgctacat cataccctac ccgttgggaa tgaccgaact
    13021 gtaaatccag caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcac
    13081 ccgccccacc cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc
    13141 tgttggcgcg acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg
    13201 cggcatcagc gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcactgc
    13261 gccccatcac cgagataaag taatccccgt cacgggttaa aacgccctta aacatcggat
    13321 cgtcattgag caaagtacgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg
    13381 taccaccggg gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga
    13441 taattttatc gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc
    13501 tgataacacc cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga
    13561 cgcgcggtgc gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggcggaggag
    13621 gaggtggcgg cgcatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc
    13681 gcttcaccgc ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg
    13741 catctttagg cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg
    13801 gcagtccctg aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat
    13861 ggctaaacgc ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac
    13921 cgttagaaac ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca
    13981 gcgtcgctac caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac
    14041 cggctaaacg cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac
    14101 ggccttgtgc ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac
    14161 cttgttgtgg ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca
    14221 taaactccag ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat
    14281 tgtcgagcgt atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac
    14341 ccagagcttg taaaaagccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca
    14401 gatagattac ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg
    14461 cctgctcatc agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg
    14521 ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa
    14581 cgcgccgcca gtacgtcagc tgcagcaccg tgcgcgacac agcccgcaca ggcggcatca
    14641 tacggcgaca gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg
    14701 ccgccgctcg ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg
    14761 gcgaccacgg ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt
    14821 ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg
    14881 gcctcgccag gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc
    14941 aggttcaatg catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt
    15001 gcttttttcc cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat
    15061 tccaggcttt cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca
    15121 gtcagcagcg gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca
    15181 cgtagcgccg cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata
    15241 attaccagcc gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt tagccagtga
    15301 gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccaactatcc
    15361 agccccagtg agtcaaaatg cagttgtccg gtaacatccc gcgcttttcc agtgagcaag
    15421 cctggtttca gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta
    15481 gcgccggttt cagccagcag gccggaaggg atatcaaccg ccgcaatcgg cgcaggatgg
    15541 gaattagcgt ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta
    15601 ccgagcagcg catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg
    15661 atctcgcctc ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt
    15721 ttgtcgctct cctgggccaa caacgtgacc tcaatgccga ccgctgtggc cagtcgcgcg
    15781 accacgtagc catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg
    15841 tcaggatacg ccgaacgaca cacctggaat gcggcttcgc cagcgcgaag catcagctca
    15901 tagagtgtaa gccccagcgc atctgccgcc tcacgttctc cgcggcggat atcgtcggcg
    15961 tgccagacgg tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca
    16021 gagcccctcg atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc
    16081 tttcagcagg taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca
    16141 tggctggaca aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc
    16201 gctcgccccc atgagttatt acccggtacg ctgcgcgtga tcagcgtgcg gatgaattac
    16261 cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc
    16321 cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc
    16381 gagatgattc agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct
    16441 attctcgagc gcccgttagc tgcaaaagct gggctcggct ggacaggtaa gcactcactt
    16501 atcctcaatc gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg
    16561 ctgcccgtgg atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt
    16621 tgcccgaccg gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat
    16681 ctcaccatcg aactggaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt
    16741 atttacggtt gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact
    16801 acagaagacg atttcagccc gcgtaagccg ctacacgcac cggaactcat tgagttattc
    16861 gcctggagcg aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt
    16921 cacctgcgct ggctgcgtaa tatcgccgtg gcattaggca atgccccctg ggatgaaacg
    16981 attctggcgg cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg
    17041 tgggcgatgg cgcagcaaat cgagaggcga aatgcgtgca tagtcgaagt gcaattgccg
    17101 aaaaaacagc gtctggttcg ggtgattgaa aaagggttac cacgtgacgc ctgattcatc
    17161 cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa gacgaataaa atcgtcaagt
    17221 gatcgactcg acaaaacgaa atcttaattt ttaacttatc tatcaataag ttaaaaaaat
    17281 actattcacc gtgcgaagtt tctttgaaat taagactaca gcctgaggct gtggataact
    17341 ctgtttacaa gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg
    17401 gatattttat tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac
    17461 cttggcaagg tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca
    17521 gcactttcaa attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag
    17581 gtcgtgctct accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc
    17641 ttggctgcct tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt
    17701 tgccgccttg tcgcaattcg aaatactgcg attgggtggt ctgtgctttg cagcactttt
    17761 caaattttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
    17821 tctaccaact gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc
    17881 tgcgtcgtta cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa
    17941 gttttttttg cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt
    18001 caaaatcacg ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc
    18061 ttgataaaat gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatc
    18121 gcctgatgcg tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc
    18181 agctctttca gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc
    18241 atgtatttaa acaggaaacg acggtcctga ccgatgctgt taccgcaaat cggcgatttt
    18301 cccgcaggca cccactgttt taaaaattcg agcgttgcca gttcagcttc ccgatcgccc
    18361 atcgtgctcg ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag
    18421 tcatccatca gcgccagctg ttcatcagac tggtgtactg caatggtcgg cccttctgcc
    18481 agaatattca ggttggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg
    18541 ggatccagac cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg
    18601 ctattttcca cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt
    18661 ttgcccatca ggggcgacca ggagtctgta cgattgagta aaaataaact ctccaaaggc
    18721 cagcagcgcc gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc
    18781 gactacgacg acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt
    18841 atgcacgctg atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc
    18901 atccgttcgc tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa
    18961 ggcgtaaatg tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt
    19021 ccggatttct acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc
    19081 tccgccattt tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa
    19141 accttgcaga ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc
    19201 atggcgttcg tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg
    19261 gtttccagcc atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc
    19321 agcatttttg ccgggcagtc tggcgtcggc aaatccagcc tgctgaatgc gttgctgggg
    19381 ctgcaaaaag agatcctgac caacgatgtc tctgacaact cggggctcgg tcagcacacc
    19441 acgaccgccg ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt
    19501 gtgcgtgagt tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa
    19561 ttccatgact atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc
    19621 tgcgctatcc gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat
    19681 caccgtattc tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat
    19741 gactgacaat taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt
    19801 tggccggatc aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa
    19861 ctttcgctac agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca
    19921 agcaagcggg caggatggct gacaaaactg gttatcgatc tgttcgttaa atactacaag
    19981 gtcgacatga aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc
    20041 tttgtccgtc cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtcctggtc
    20101 atgcctgccg atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa
    20161 gccaaaggcc acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac
    20221 ctgttccgca acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccatcgcgta
    20281 cacatgccgt gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc
    20341 gttaaccatc tcaccgctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt
    20401 tgcctgttcg ataccgaatt tggcccgatg gcgcagattc tggtcggggc gacgattgta
    20461 ggcagcattg agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag
    20521 cgctggacct ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa
    20581 atgggccgct ttaaactcgg ctctaccgtt atcaacctgt ttgcaccggg taaagtgaat
    20641 ctggttgagc aactggaaag cctgtctgtc acgaaaattg gccagccgct ggcagtatct
    20701 accgaaacct ttgttacgcc agacgctgaa cccgccccac ttcctgctga agagatcgaa
    20761 gcagaacacg acgccagccc attggttgac gataaaaaag accaggtcta atccatcaaa
    20821 ggaaacgctg acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg
    20881 gcgtacgccg cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa
    20941 gcggcgaaac ccgcacagcc ggaagtcgta gaggcgctcc agtctgcctt aaatgcgctt
    21001 gaggaacgaa aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat
    21061 ccgaaactct ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacga gccgcgcagc
    21121 gtgtcgccgg gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag
    21181 ttgctggata aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat
    21241 tcgctgaatc aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag
    21301 cgccgcctgg gaacgcttac tggcaatacc ccgctcaatc aggcacaaaa tttcgcgttg
    21361 cagtctgact ccgcacgcct gaaagcgctc gttgatgaac tggaactggc gcagctgtct
    21421 gccaataacc gtcaggaatt agcgcgctta cgctcagagc tggcggaaaa agagagccag
    21481 caactggatg cgtatttgca ggccttacgt aatcagttga acagccaacg tcagcttgag
    21541 gcggagcggg cgctggaaag taccgaattg ctggcagaaa acagcgccga tttgccgaaa
    21601 gacatcgtcg cgcaattcaa aattaaccgc gaactatcgg cggcactgaa tcaacaggcg
    21661 cagcggatgg atctcgttgc ctcgcaacag cgccaggcca ccagccagac gttacaggtt
    21721 cggcaggcgc tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caacctgctc
    21781 ggcgaagcgt tgcgggcgca ggtggcacgg ctgccggaaa tgccgaaacc acaacagctt
    21841 gataccgaaa tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa
    21901 cagccgctgc tacggcaaat tcatcaggcc gacggtcagc cgctgaccgc cgagcaaaac
    21961 cgtattctgg aagcacagct acgcactcag cgtgagttgc tgaactcatt gttgcagggt
    22021 ggcgacacgc tactgctgga actgaccaag ctgaaagtct ccaacgggca acttgaggat
    22081 gcgctgaaag aggtgaatga agcgacgcat cgctatctgt tctggacctc tgacgtgcgc
    22141 ccgatgacca tcgcctggcc actggaaatc gcccaggatc tgcgtcgtct catttcgctg
    22201 gacaccttca gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacgatt
    22261 ttgccgctgt ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc
    22321 acccgttttc ttgaacgttc ggcggcgaaa gtcggcaaag tgactcagga tcacttctgg
    22381 ctgacgttgc gcactctttt ctggtcgatt ctcgtcgcgt caccgctgcc ggtgctgtgg
    22441 atgacgctgg gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc
    22501 gatggcgtaa cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc
    22561 cgcccgaacg gcttgtttat cgctcatttt ggctggccgc gcgaacgtgt ttcccgtggg
    22621 atgcgctact acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg
    22681 ttcgataacc tcgacgaccg tgaattctcc ggttcgctgg gacggctttg ctttatcctc
    22741 atttgcggtg cgctggcggt ggtcaccctc agcctgaaaa aggccgggat cccgctgtac
    22801 ctcaacaaag agggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt
    22861 ggcgcaccac tggttgccat tctggcgtca gcggtgggtt atctggcaac ggcgcaggca
    22921 ctgttagcga ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac
    22981 gttatccgcc gctggatgct gatccagcgt cgcaggctgg cgtttgaccg ggcgaagcat
    23041 cgccgggcag agatgttagc gcaacgcgcg cgtggcgaag aggaagcgca tcatcacagt
    23101 agcccggaag gggcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg
    23161 caatccttgc ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg
    23221 ctgtggtcag aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc
    23281 acctccacgg tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt
    23341 gccattctgg tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa
    23401 ctggcgattt tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc
    23461 accaaatatc tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag
    23521 tggtcgaaat tgcagtggct ggttgccgcg ctcggtgttg gtctcggttt tggtttgcag
    23581 gagattttcg ccaactttat ctctggcctg attatcctgt tcgaaaaacc gattcgcatt
    23641 ggcgatacgg taacgatccg cgatcttacc ggtagcgtga cgaaaattaa cacccgcgcc
    23701 accaccatca gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc
    23761 gagcagttta tcaactggtc gctctctgac tcggtcacgc gcgtggtgtt gacgataccg
    23821 gcccctgccg atgccaatag cgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc
    23881 tgctcgctgg tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacagggg
    23941 attcagattt tcgagctgcg tatttacgcc gctgagatgg gtcaccgtat gccgctacgc
    24001 catgagatcc accagctgat tctggcaggc ttccacgccc acggtatcga tatgccattc
    24061 ccgcccttcc agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg
    24121 tctgcgggca aaggtcgtca ggcgggaagt ttgtaagttt gtgtgccgga tgaaccaatc
    24181 cggctcacaa acaaaactga tagcgcagaa aaaactgcca cagccagtag ccgaaaatag
    24241 ccagcgcggt ggcgtacata aaccagccgc gtttttttcg ccacaaagcg aaccagagaa
    24301 aagggaaaaa gagcggtaac gtcattatca caccaacatc acgggtatcg tcttcttcga
    24361 gatacatcaa cacatcgcag acgttgttaa tcccgcgtcc atcaatcatc cactctttgt
    24421 ccagcgcgga agtgatggtt atccagagga acgccacaat aaaccagatg cagcataaga
    24481 caaacatgcg cgcactcata aggtaaccgg tcgcttaatt tcgctatagc gcatacgaaa
    24541 caaatcgccg tatcccacta attccccgcg aaaaaccaga catcggataa gataagcgca
    24601 ataaatatcc ggcaggtttc cccaccgttt accatcgcga tctttgaggg tgttaatggt
    24661 gctacccacc accatcgcca ctttacgggt gtggcaggca tcgacaaaag agagaatcgt
    24721 ctgctctatt tccgctacat ccccggcaga aaattgcagc gccctgattt catcttcggt
    24781 tattgccggg tcccggctgg tgtcatccat aacatttcct gtctttacgc cctatcgtca
    24841 ggtagacgat agggcatcgg gttattccac cgcccactgc ggattttttg ccactctgct
    24901 gcaatagttc tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat
    24961 ccacaatgtg ctgtcccagt ctccggcttc aaccacaggt tgaatgatgg tgaagacgtt
    25021 cgcaaatgtc accaccagta cgacaaccac tgttgcaatc attgccgaca tatgcgtttt
    25081 gaaaatcaca aatggtctgt cgagatcctg acgtgcttta aaaaatggga aagccagcgc
    25141 gaggaacagg taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa
    25201 cgccgatgcg gtaccgccac caaacgaaac cagcaggatg aagacagtaa ccaacccgca
    25261 ctgcatccac atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg
    25321 ccacaacgct ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa
    25381 gaacgcaccg gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc
    25441 cagcgacaat gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct
    25501 cttcatcagc acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg
    25561 ccagtttgtg ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat
    25621 agcggcaaaa acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag
    25681 accaccaacc gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat
    25741 tgccagaccg gactgataac ctgggttcgg tgatgcaagg aaattaatat cctgcgcgaa
    25801 atgcccacca tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca
    25861 cattactgca ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc
    25921 aacgacggtg accagaatca tccatgccac tgccagcaga ccaaccacct gcgtaggctc
    25981 cagtccagca atacgccagt gctgggtcat gtcgctacca tagaggaatg ttgagaacgg
    26041 tacccaaact ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa
    26101 cgtaccaatg aaggcaaaac gtggtccgac actattattc atccaggaat agataccgcc
    26161 ttcttctttg cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa
    26221 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
    26281 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
    26341 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
    26401 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
    26461 tacctggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
    26521 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
    26581 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
    26641 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
    26701 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
    26761 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgtcaattc atggaaacca
    26821 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
    26881 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
    26941 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcag cgtgtcgcgg
    27001 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
    27061 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
    27121 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
    27181 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
    27241 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
    27301 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
    27361 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
    27421 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
    27481 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
    27541 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
    27601 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
    27661 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
    27721 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
    27781 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
    27841 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
    27901 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
    27961 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
    28021 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
    28081 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
    28141 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
    28201 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
    28261 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
    28321 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
    28381 gtcatgttcg cggcgtggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
    28441 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
    28501 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
    28561 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
    28621 aaggctgccg cggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
    28681 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
    28741 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
    28801 ccacgccgcg tggcgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
    28861 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
    28921 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
    28981 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
    29041 tgcacggtgc aaaccgtctg ggttctaact ccctggcgga actggtggtc ttcggccgtc
    29101 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
    29161 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
    29221 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
    29281 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
    29341 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
    29401 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
    29461 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
    29521 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
    29581 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
    29641 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
    29701 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
    29761 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
    29821 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
    29881 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
    29941 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
    30001 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
    30061 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
    30121 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
    30181 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
    30241 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
    30301 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
    30361 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
    30421 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
    30481 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
    30541 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
    30601 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
    30661 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
    30721 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
    30781 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
    30841 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
    30901 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
    30961 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
    31021 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
    31081 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
    31141 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
    31201 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
    31261 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
    31321 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
    31381 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
    31441 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
    31501 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
    31561 aagcgatccc acaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
    31621 actattacat ctcgcaacct acactgctgg cggcctgcca ttgcaggtgc cggatgaggt
    31681 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggcgccagg
    31741 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
    31801 gtctggtttg agttttgagc aggcgatgca aacacgtgtc ttccagccac tcaaactcaa
    31861 ccatacgtgg attaatgtac cgcccgcaga agaaaagaat tacgcctggg gatatcgcga
    31921 aggcaaggca gttcatgttt cgccaggggc gttagatgct gaaacttatg gtgtgaagtc
    31981 gaccattgaa gatatggcct gctgggtacg aagcaatatg aatccccgtg atatcaacga
    32041 caaaacactt cagcaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
    32101 gtatcagggc ctgggctggg aaatgctgga ctggccggta aatcctgaca gcatcattaa
    32161 cggcagtggc aataaaattg cactggcagc acaccccgta aaagcgatta cgcccccaac
    32221 tcctgcagta cgcgcatcat gggtacataa aacaggggca accggcggat ttggtagcta
    32281 tgtcgcgttt attccagaaa aagagctggg tatagtgatg ctggctaaca aaaactatcc
    32341 caatccagcg agagtcgccg ccgcctggca gattctcaac gctctacagt aaaatttcat
    32401 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
    32461 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
    32521 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtaacagta gtgaataatt
    32581 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
    32641 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
    32701 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
    32761 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
    32821 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
    32881 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
    32941 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
    33001 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
    33061 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
    33121 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctactggt aacgatttca
    33181 tcgcccaggc aagtagcgcc atactgacaa tcatcgccgt cacggtaata acactcggcg
    33241 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
    33301 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
    33361 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
    33421 gtggcagggg aatttcaact attaccttag tgactatcgc agaaaaaaca aacggcacga
    33481 catacctgtc gtaccgttat tgcttattgc tattgctgcg ctttcgttgc tgcgccagaa
    33541 atcgcgttac cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt
    33601 aatactgttg aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg
    33661 tcttcctttt aggtaatgtt atttatgttt gcctatagca aattaataat agacaacatc
    33721 gccaaaaatg acggaaatct gatgattttt cggaagagaa gaaaaattag ctggcagcgc
    33781 gagagatgga gttgccgaga tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc
    33841 ccgtgagcag cgtgctggca agcaaaacaa gaacgataag gcgtttcatc atctctgacc
    33901 tgaataagtg atggtgcagc ctgcaggccg caccacaacc gcattacttc acgcgagaga
    33961 cgtattcacc agagcgggta tccactttga tgacttcgcc gatttgtacg aacagcggaa
    34021 ccttaaccac agcgccagta gacagggtag ccggtttgcc gccagtacct gcggtatcac
    34081 ctttcaggcc cggatcggta tcaacgattt ccagttcaac gaagttcggc ggagtaacgg
    34141 agatcggctg accattccac agagttacga tacactctgc ctgatccagc agccatttag
    34201 cgttgtcgcc aattgctttt gcatcagcag acagctgctc gaaagtttcg ttgttcatga
    34261 agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt catatcgaca acatcagcgc
    34321 cttcagcgga atcagtagat ttgaaggttt tttctacgcg agtaccggtc agcagacgac
    34381 gcagtttaac gcgagcaaat gcctggcctt tacccggttt tacgaattca ctcgcttcaa
    34441 ccgcgtaagg ttcgccgtct aacatgattt taagaccagc acgaaaatcg ttgctatagt
    34501 acgttgccat aaggccctct gaaatttgtt aattggtagc taagccacaa aatggcgcat
    34561 attgtaaccc taaatacccc atccagagaa gattggttaa cgcaacttgc cgatgttgtg
    34621 accgatcctg atgaacttct gcgtcttttg aatatagacg cggacgaaaa actgttggcc
    34681 ggacgcagcg ccaaaaagct gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg
    34741 gagaaaggca atccggacga tcctcttttg cgtcaggtac ttacctcgca agatgagttt
    34801 gtcgtcgcgt ccggattctc caccgacccg ctggaagaac agcacagcgt agtgcctggt
    34861 ttgttgcata aataccacaa ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat
    34921 tgccgctatt gcttccgtcg ccacttcccc tatgccgaaa atcagggcaa caagcgtaac
    34981 tggcagactg ctcttgagta tgttgctgcg catccggaac tggacgagat gattttctcc
    35041 ggcggcgatc cgctgatggc gaaagatcac gagctggact ggttgctcac acaactggaa
    35101 gccatcccgc atatcaaacg tctgcggatt cacagccgtc tgccgattgt gatcccggca
    35161 cgtatcaccg aggcgctggt tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg
    35221 aatcacatca atcatgccaa tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg
    35281 cgccgtgtcg gtgtcaccct gcttaaccag agcgttctgt tacgtggtgt gaacgataac
    35341 gcacaaacgc tggcaaacct gagtaatgcg ttgttcgatg ccggcgtaat gccgtattac
    35401 ctgcatgtgc tcgataaagt acagggcgcg gcgcatttta tggtgagtga tgacgaagca
    35461 cggcagatta tgcgtgagtt gctgacactg gtgtcgggtt atctggtgcc gaaactggcg
    35521 cgagaaatcg gcggcgaacc cagcaaaacg ccgctggatc tccagctacg ccagcagtaa
    35581 ctgattaaac agggaagtta aacattagcc tgatgcgcta cgcttatcag gcctacattg
    35641 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
    35701 tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaactggga aatttaatct
    35761 ttgcccgttg cataaccttt aattattcat atacattgct gttgttactc tttatatagc
    35821 caacaccaat aattaac
//
LOCUS       DAZFRJ010000025        35829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000025 DAZFRJ010000000
VERSION     DAZFRJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.025"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1169
                     /locus_tag="ACHKUN_001156"
     CDS             <1..1169
                     /locus_tag="ACHKUN_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6659454.1"
                     /translation="ARKNGTYTDLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANISQ
                     PDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQESEQNDQKTEQNSPNAQ
                     QNEPETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFDEENQPEVQENDPEEME
                     GTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNITSTSRAGIHLMIDLETMGKN
                     PDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAILT
                     DEIPLDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRYTNDR
                     DVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSAIWQKLLPSQADF"
     gene            1237..1479
                     /locus_tag="ACHKUN_001157"
     CDS             1237..1479
                     /locus_tag="ACHKUN_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO6659455.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            1457..2476
                     /locus_tag="ACHKUN_001158"
     CDS             1457..2476
                     /locus_tag="ACHKUN_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6659456.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            2884..3543
                     /gene="yccA"
                     /locus_tag="ACHKUN_001159"
     CDS             2884..3543
                     /gene="yccA"
                     /locus_tag="ACHKUN_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO6659457.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            3634..3963
                     /gene="tusE"
                     /locus_tag="ACHKUN_001160"
     CDS             3634..3963
                     /gene="tusE"
                     /locus_tag="ACHKUN_001160"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO6659458.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(3960..4238)
                     /gene="yccX"
                     /locus_tag="ACHKUN_001161"
     CDS             complement(3960..4238)
                     /gene="yccX"
                     /locus_tag="ACHKUN_001161"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO6659459.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            4333..5523
                     /gene="rlmI"
                     /locus_tag="ACHKUN_001162"
     CDS             4333..5523
                     /gene="rlmI"
                     /locus_tag="ACHKUN_001162"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO6659460.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            5581..5898
                     /gene="hspQ"
                     /locus_tag="ACHKUN_001163"
     CDS             5581..5898
                     /gene="hspQ"
                     /locus_tag="ACHKUN_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO6659461.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(5943..6356)
                     /gene="yccU"
                     /locus_tag="ACHKUN_001164"
     CDS             complement(5943..6356)
                     /gene="yccU"
                     /locus_tag="ACHKUN_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO6659462.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            6529..7191
                     /gene="yccT"
                     /locus_tag="ACHKUN_001165"
     CDS             6529..7191
                     /gene="yccT"
                     /locus_tag="ACHKUN_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO6659463.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7287..7745
                     /gene="mgsA"
                     /locus_tag="ACHKUN_001166"
     CDS             7287..7745
                     /gene="mgsA"
                     /locus_tag="ACHKUN_001166"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO6659464.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(7777..9831)
                     /gene="helD"
                     /locus_tag="ACHKUN_001167"
     CDS             complement(7777..9831)
                     /gene="helD"
                     /locus_tag="ACHKUN_001167"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO6659465.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            9954..10400
                     /gene="yccF"
                     /locus_tag="ACHKUN_001168"
     CDS             9954..10400
                     /gene="yccF"
                     /locus_tag="ACHKUN_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO6659466.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            10410..12572
                     /gene="yccS"
                     /locus_tag="ACHKUN_001169"
     CDS             10410..12572
                     /gene="yccS"
                     /locus_tag="ACHKUN_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO6659467.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(12535..13164)
                     /gene="sxy"
                     /locus_tag="ACHKUN_001170"
     CDS             complement(12535..13164)
                     /gene="sxy"
                     /locus_tag="ACHKUN_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO6659468.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            13383..13892
                     /gene="sulA"
                     /locus_tag="ACHKUN_001171"
     CDS             13383..13892
                     /gene="sulA"
                     /locus_tag="ACHKUN_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO6659469.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            14249..15289
                     /gene="ompA"
                     /locus_tag="ACHKUN_001172"
     CDS             14249..15289
                     /gene="ompA"
                     /locus_tag="ACHKUN_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO6659470.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(15364..15816)
                     /gene="matP"
                     /locus_tag="ACHKUN_001173"
     CDS             complement(15364..15816)
                     /gene="matP"
                     /locus_tag="ACHKUN_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO6659471.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            16002..17762
                     /gene="ycbZ"
                     /locus_tag="ACHKUN_001174"
     CDS             16002..17762
                     /gene="ycbZ"
                     /locus_tag="ACHKUN_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6659472.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            17831..18349
                     /gene="fabA"
                     /locus_tag="ACHKUN_001175"
     CDS             17831..18349
                     /gene="fabA"
                     /locus_tag="ACHKUN_001175"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO6659473.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18419..18586)
                     /gene="rmf"
                     /locus_tag="ACHKUN_001176"
     CDS             complement(18419..18586)
                     /gene="rmf"
                     /locus_tag="ACHKUN_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO6659474.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18842..19405)
                     /gene="pqiC"
                     /locus_tag="ACHKUN_001177"
     CDS             complement(18842..19405)
                     /gene="pqiC"
                     /locus_tag="ACHKUN_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO6659475.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            complement(19402..21042)
                     /gene="pqiB"
                     /locus_tag="ACHKUN_001178"
     CDS             complement(19402..21042)
                     /gene="pqiB"
                     /locus_tag="ACHKUN_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO6659476.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(21047..22300)
                     /gene="pqiA"
                     /locus_tag="ACHKUN_001179"
     CDS             complement(21047..22300)
                     /gene="pqiA"
                     /locus_tag="ACHKUN_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO6659477.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(22430..24337)
                     /gene="uup"
                     /locus_tag="ACHKUN_001180"
     CDS             complement(22430..24337)
                     /gene="uup"
                     /locus_tag="ACHKUN_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6659478.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(24349..26457)
                     /gene="rlmKL"
                     /locus_tag="ACHKUN_001181"
     CDS             complement(24349..26457)
                     /gene="rlmKL"
                     /locus_tag="ACHKUN_001181"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO6659479.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            26701..27810
                     /gene="ycbX"
                     /locus_tag="ACHKUN_001182"
     CDS             26701..27810
                     /gene="ycbX"
                     /locus_tag="ACHKUN_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO6659480.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(27807..28349)
                     /gene="zapC"
                     /locus_tag="ACHKUN_001183"
     CDS             complement(27807..28349)
                     /gene="zapC"
                     /locus_tag="ACHKUN_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO6659481.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(28523..29533)
                     /gene="pyrD"
                     /locus_tag="ACHKUN_001184"
     CDS             complement(28523..29533)
                     /gene="pyrD"
                     /locus_tag="ACHKUN_001184"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO6659482.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(29644..30381)
                     /gene="ycbF"
                     /locus_tag="ACHKUN_001185"
     CDS             complement(29644..30381)
                     /gene="ycbF"
                     /locus_tag="ACHKUN_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6659483.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(30347..30862)
                     /gene="ycbV"
                     /locus_tag="ACHKUN_001186"
     CDS             complement(30347..30862)
                     /gene="ycbV"
                     /locus_tag="ACHKUN_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6659484.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(30870..31412)
                     /gene="ycbU"
                     /locus_tag="ACHKUN_001187"
     CDS             complement(30870..31412)
                     /gene="ycbU"
                     /locus_tag="ACHKUN_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6659485.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(31424..32494)
                     /gene="elfG"
                     /locus_tag="ACHKUN_001188"
     CDS             complement(31424..32494)
                     /gene="elfG"
                     /locus_tag="ACHKUN_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6659486.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(32485..35085)
                     /gene="elfC"
                     /locus_tag="ACHKUN_001189"
     CDS             complement(32485..35085)
                     /gene="elfC"
                     /locus_tag="ACHKUN_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO6659487.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(35110..35811)
                     /gene="elfD"
                     /locus_tag="ACHKUN_001190"
     CDS             complement(35110..35811)
                     /gene="elfD"
                     /locus_tag="ACHKUN_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6659488.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         8838 a         9281 c         8587 g         9123 t
ORIGIN      
        1 ttgcccgcaa aaacggaaca tacacggact taccgcaaaa tgtggatacc gctgaagatt
       61 ccgtacaacc ggagccggta aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag
      121 ccaatatcag ccagcctgac gccgataaat tgcttgctgc atcacgtggt gaatttgttg
      181 aagggattag cgatccgaat gatccgaaat gggtgaaggg gattgaaacc tgcgattctg
      241 tgaaccagaa ccagcaagaa tcggaacaga acgaccagaa aacggaacaa aacagcccaa
      301 atgcgcaaca aaacgagcca gaaacgaaac aggagccgga aaaagcctgc accgcctgcg
      361 gtcagaccgg cggcggtagc tgccctgatt gtggtgcggt gataagtgac gcaacatacc
      421 aggaaatatt cgatgaagag aatcagcctg aagttcagga aaatgatccg gaggaaatgg
      481 aaggcactgc gcatcagcac aaggagaaca ctggcggcaa tcagcatcat gccagcgata
      541 gtgaaactgg cgaggcgtca gatcccttaa ttaaggcgaa cggtcatcat aatatcacat
      601 ccaccagcag agcggggatt catctgatga tcgaccttga aaccatggga aaaaatcctg
      661 atgccccgat tatctcaata ggtgcaatat ttttcgatcc gcaaaccgga gagatggggc
      721 cagaattcag caaaactatc gatctggaaa ctgctggcgg agtcattgat cgggacacca
      781 ttaagtggtg gctgaaacag tcacgcgaag cgcaatccgc cattcttacc gatgaaatcc
      841 cgttagatga tgcactgctg caattacggg aatttatcga cgaaaactcc ggtgaatttt
      901 ttgttcaggt ctggggtaac ggtgcaactt tcgacaacgt gattttacgc cgttcatatg
      961 aacggcaggg gatcccctgc ccgtggcgtt acaccaatga tcgcgatgta agaacgatgg
     1021 ttgctctggg actggtgatg gatttcgacg caagaacgac tattccattc gaaggtgaac
     1081 gccataacgc tctgaacgat gcacgttacc aggcgaaata cgtttcagcc atctggcaaa
     1141 aactgctccc gagtcaggct gatttttgat gttcaaccca tatcgccgcc caccagctat
     1201 agtggcggcg gtcatgctgt aaaggcacgt gaccacatgt acgaattaac tctatctcca
     1261 gcagagatta aagagatcac gaaatacgag cgatacacaa aacagcaaca ccagttaaga
     1321 ctgcacggca tcccatttgt aatcggccct aaaaacgaac ccatagttct ccgcagggat
     1381 attccacacg gtctgacaac gatgccaaaa acatctgaac tggtttctgc tgaacccgat
     1441 tttgaggcgc tgaacaatgg gaagaccaag aaaaaataaa aaagataatg tactgccacc
     1501 gcgggttaga tcgaatggtt acagttacgt gtggaaaccc gaaggaagta caagaagtat
     1561 agggctagga agagtgcgga aaaccagcgt agctaaagtc tggcaaaatt atgaactgga
     1621 aaaagcaaaa ctccacaaca taatgaccgt agctaaatta tggcacatgt ttatggactc
     1681 ccctgcattt acagaactgg ccccccgaac ccaaaaagat tatcgacaac atcagaaggc
     1741 gttgctgatg gtattcggaa aagtgcttgc tgataatgtc aaaactgagc aggtaagaat
     1801 tttcatggat aaacgagggc ttgagagcaa gacccaggca aatcatgaac tggcaagcct
     1861 gagtcgagta tacgggtggg gatatgagcg tgggtatgtg aagaataacc catgcaaagg
     1921 agtcagaaaa ttctctctta aagcccgcac tgtttacatc accgatgaac agtatgcggc
     1981 gatatatgcg gaagcaattc cacagttacg cattgcaatg gagatttcct atctctgtgc
     2041 ggcaagactc ggtgatgtgc ttgagttgaa atggcaggat attatggata aagggatcta
     2101 cattgagcaa aacaaaaccg gcaccaaaca aatcaaggaa tggtcaccgc gattacgtac
     2161 agcgatccag ttagcccgaa atgtatcttc ctgtacatgc gaatatgtga tcaatacaac
     2221 caaaggcggg aaagtcatag ctaaaacgct gaataactgg tggaatcagg ctaaacgcgc
     2281 agccgagcaa aaagttggcg ttccgttcgg gtgcaatttt cacgacataa aagccaaggg
     2341 gatctcagat tacgaaggca gcagtcgcga caaacaaatt ttcagcgggc ataaaacaga
     2401 aaatcaggtg ttgatttacg atcgtaaaac aaaaatcaca ccaacactgg atttgccgct
     2461 cgtggttagc aagtagacaa tccagaaaaa tttctggcaa aaagacttgg tatactagct
     2521 atgagcaact tcctaatacc ttgatttttc ttggtgttaa atttgattaa cttttcagca
     2581 atttcacgta ttgcactgat ttttatttgt ttttcttcgg tcttgaaaac cggcgacccg
     2641 aaagggttcc agagttcgaa tctctgcgct tccgccaaat aagataaggg gttagctaaa
     2701 tgctaacccc tttttctttt gcctgtcgaa attctcaggg cgttatattt gcttaatgac
     2761 ctgataatcc gctgttaaac ctgttccaga tcaaatgcgt aaagatgggt aaaacttctg
     2821 ggtgccctta cgcattatca ttatgctgct taattaatta catctgtcat agagagtgac
     2881 tcaatggatc gtattgttag ttcttcacat gaccgtacat cactgcttag cacccataag
     2941 gtgctgcgta atacctattt tctgctgagc ctgacgctgg ccttttcggc aattaccgca
     3001 actgccagta cggtgctgat gctgccatct ccgggtctga ttctgacgct ggtgggtatg
     3061 tatggtttga tgttcctgac ctataaaacg gcgaataagc cgaccgggat tatctccgca
     3121 ttcgccttta ccggttttct gggttatatc ctcgggccta ttctgaacac ctatctgtct
     3181 gccggaatgg gtgacgtaat cgctatggca ctgggcggaa cggcgttagt gttcttctgc
     3241 tgctctgcat atgtgctgac cacccgcaaa gatatgtcgt tcctcggcgg tatgctgatg
     3301 gcgggtattg tggtggtgct gattggtatg gttgctaata tcttcctgca gctgcctgct
     3361 ctgcatctgg cgatcagcgc ggtcttcatt ttgatctcct ctggcgctat tttgtttgaa
     3421 accagcaaca tcgttcatgg cggtgaaacg aactatattc gtgccacggt tagcctgtat
     3481 gtttcgctgt acaacatctt cgtcagcctg ctgagcattc tgggcttcgc cagccgcgat
     3541 taatctcacc cgctaacact ttcatagcct cgctttatgc ggggctttgt tttttgttac
     3601 actgccgcca gtctgattaa ggtagtaata attatgctga tcttcgaagg taaagagata
     3661 gaaacggata ccgaaggcta tctcaaagaa agcagccagt ggagtgagcc gctggcagtg
     3721 gtgattgcag agaacgaagg gatttcgctg tcaccggaac actgggaagt ggtgcgtttt
     3781 gtgcgtgatt tctatctgga attcaatact tctccggcga ttcgtatgct ggtaaaagcg
     3841 atggcgaata aatttggcga agaaaaaggt aacagccgct atttataccg actgtttccg
     3901 aaaggtccgg ctaagcaagc caccaaaatt gctggcctgc caaaaccggt aaaatgtatt
     3961 taacggatac gaaaatcggt taattccccc gagggatgat gcggctcgct gagcacccgt
     4021 tctacacgcg cagaacgcgg gccgccactc tttaaccaca gcattaattt ttccacctgc
     4081 ccttcttcac cgcaggcaac cacttccacg ctgccatcgt caagattttt ggcgtacccg
     4141 gttaaaccca gtcttttcgc ttcgtactgt gtggtgtagc gaaatcctac gccctgaact
     4201 cgcccgtaaa cccaggcaat tatgcagact ttcgacattg ctgctctcct tatccttaat
     4261 ggacgcgttg caaatccatg gaaaactccg gacaatgacg cccatttctt tgcatcgaca
     4321 gaaaagtaaa ttatgagtgt acgtttagtg ttagccaaag ggcgcgaaaa atcattactt
     4381 cgtcgccatc cgtgggtctt ttccggggcc gttgcccgca tggaaggtaa agccagcctc
     4441 ggtgaaacca tcgatattgt tgatcatcag ggaaaatggt tagcacgcgg cgcttattca
     4501 ccagcttcgc aaatccgggc gcgcgtctgg acgtttgacc cgtctgagtc tatcgacatt
     4561 gctttttttt cccgccgttt gcaacaagca caaaaatggc gtgactggct ggcgcaaaaa
     4621 gatggcctcg acagctatcg tttaatcgcc ggagaatctg atggcctgcc gggtattact
     4681 atcgatcgtt tcggtaattt tctggtgctg caactgctga gtgctggggc agaatatcag
     4741 cgcgcggcat tagttagtgc cctgcaaacg ctgtacccgg aatgtgcgat ttacgatcgc
     4801 agcgatgttg cggtacgtaa aaaagaaggg atggagctga cccagggcct cgtcaccggc
     4861 gagttgccgc ctgccctgct gccgattgaa gaacacggca tgaagctgct ggtggatatt
     4921 cagcacggac acaaaacggg ctactacctg gaccagcgtg atagccgcct ggctacccgc
     4981 cgctacgttg aaaataaacg tgtgctgaac tgtttctcct ataccggtgg tttcgccgta
     5041 tcggcactga tgggcggttg cagccaggtt gtcagcgttg atacctccca ggaagcgctg
     5101 gatattgcac ggcagaacgt tgagctgaac aaactggatc tgagcaaggc tgagtttgtc
     5161 cgtgatgatg tctttaaatt gctgcgtact tatcgcgatc gcggtgaaat atttgacgtt
     5221 atcgtgatgg acccgccgaa gtttgttgag aataaaagcc agttgatggg cgcgtgtcgt
     5281 ggctataaag atatcaacat gctggcgatt cagttgctga atgaaggcgg tattctcctg
     5341 actttctcct gttccggtct gatgaccagc gatttatttc agaaaatcat cgcggatgcc
     5401 gcaattgatg ccggccgtga tgtacaattt atagagcagt tccgtcaggc agccgatcat
     5461 ccggtgatcg ctacctatcc ggaagggcta tatctgaaag ggtttgcctg tcgcgtcatg
     5521 taacttgaaa tgtggaattt tacccttata tcaaaggtaa agatttcccg ggaggtgact
     5581 atgattgcca gcaaattcgg tatcggccag caggtccgcc attccctgtt aggttatctc
     5641 ggagtggtcg tggatatcga cccggtttat tcgctctctg aaccgtcgcc tgatgagctg
     5701 gcggtgaatg acgagcttcg cgccgctccg tggtatcacg tggtgatgga ggacgataac
     5761 ggcctaccgg ttcataccta cctggctgaa gcccagctaa gtagcgaatt gcaggatgaa
     5821 catcctgaac agccatccat ggatgagctg gcgcagacca tccgcaaaca actccaggcc
     5881 ccgcgtctgc gtaactgatc cctgaacgaa tccccggaag gtttttgcct tccggggatt
     5941 ttttatttgg ccaggcccag gcgagggatc tcaatagccg ggcagcgatc catcaccaca
     6001 ttcaacccgg catcccgtgc cagtactgcc gcttgctcat taatcacccc cagttgcatc
     6061 cagagggttt tcgcgccaat ggcaatagct tcctgtgcca cgccccacgc cgcttctgaa
     6121 ttacggaaaa catcgaccat atcgaccttt tccggaacgt ctgccagcgt gccatacccc
     6181 tgctggccca gcagcgtttt gccggcaact tttggcgaaa ccggaatgac gtgatacccc
     6241 tggtcgagta gatatttcat cacgcgatag ctgggacgat cgggtttgtc gcttgccccc
     6301 accagcgcaa tggtgtgagt ggacgttaaa atgcctgcaa tatcggtttc tttcattatt
     6361 tcctccagac tgtttaacaa agtgtacgac aaagccgaca tgccaaccat gcagctaata
     6421 cgaatgaatg agagcaaaag cgggaaatat gtctatatgt tagtaagtat gattaaagaa
     6481 aaattttgac acattattat tcgggcggtc ttttgacagg agctacctat gaaaaccggc
     6541 atcgtgacca ccttgattgc attatgtttg ccggtatccg tttttgccac cacgctgcgg
     6601 ctttcaaccg atgtcgatct tctggtactc gacggcaaga aggtttccag ctctctgctg
     6661 cgcggtgcgg acagcattga actggataat ggaccgcatc agttagtgtt tcgcgttgag
     6721 aagacgattc atctttccaa tagtgaagaa cggctgtata tctccccacc gctggtggtc
     6781 agttttaata cccagctcat caaccaggtc aattttcgcc tgcctcgcct ggagaatgag
     6841 cgggaagcta accattttga tgccgcgccg cgccttgaat tgttggatgg cgatgcgacg
     6901 ccgattccgg taaagctgga tattctcgcc attacctcaa cggcaaaaac gattgattat
     6961 gaggtcgagg ttgaacgcta taacaaatcc gcgaaacgcg cttcactacc gcaatttgcc
     7021 acgatgatgg cagatgacag tacgctgctt tcgggtgttt ccgagctgga tgctattcca
     7081 ccgcagtctc aggtgctcac agaacaacgg ctgaagtatt ggtttaaact ggctgaccca
     7141 caaacgcgaa atactttcct gcagtgggcg gaaaaacaac catcttcctg agatttttgt
     7201 gcctgtgcgc aggctttttc ggtctttatc ttgcagcgat aagtgcttac agtaatctgt
     7261 aggaaagtta actacggatg tacattatgg aactgacgac tcgcacttta cctgcgcgga
     7321 aacatattgc gctggtggca cacgatcact gcaaacaaat gctgatgagc tgggtggaac
     7381 ggcatcaacc gttactggaa caacacgtac tgtatgcaac aggcactacc ggtaacttga
     7441 tttcccgtgc aaccggcatg aacgtcaacg cgatgttgag tggcccaatg gggggtgacc
     7501 agcaggttgg cgcattgatc tcagaaggga aaattgatgt attgattttc ttctgggatc
     7561 cactaaatgc cgtgccgcac gatcctgacg tgaaagcctt gctgcgtctg gcgacggtat
     7621 ggaacattcc ggtcgccacc aacgtggcaa cggcagactt cattatccag tcgccgcatt
     7681 tcaacgacgc ggtcgatatt ctgatccccg attatcagcg ttatctcgcg gaccgtctga
     7741 agtaatattg cacaggtggc aaacgccacc tgtttcttac ggttttctcg ccaccggcac
     7801 atccagattt ttcagtattt ccacaaaggg agagggattc tctttgttaa acagtgccca
     7861 tacccgatgg cgtgcccggg tcagcgccac gtacattaac cgccgttctt cagcgtccgg
     7921 gaaatcctca accggtggca gtagcgcctc ttccataatc gactcccgcg ccgcagccgg
     7981 aaaaccatca ctcccctcct gcaagccaac gatgatgacg taatccgcct gttgcccttt
     8041 gctggcatga atggtcataa agtcgatttg caacttcggc cagcgtgttg ccgctttttc
     8101 caggctggca ggcctcatgt gatggtaacg cgccaggata agaatgcgct cttccggttt
     8161 ggcataacca gagagcttat ccagcaaagc gtcaagttga ctctcatcca ataacgtgac
     8221 ggctttttcg tctccattgg ttaagctgtt tagcggcttt ttcaactggc ctgggttctg
     8281 ctgaataaac cggtttgcca cctcaccgat acgactgtta aaacggtaag tcgtgtctaa
     8341 atcacagcga tcgccttcac caaagttttc atggaaagcg gtggtgagcg acatttgcgc
     8401 gccgctgaat cggtaaatcg cctgccagtc atcaccaacg gcgaacaacg tcgtctgact
     8461 gttttgcttg cgtaatgccg ctaacaacgc tgcccgctgc ggcgagatat cctgaaattc
     8521 atcaaccaga atatgcttcc acgggctgat aaagcgacct ttctccagaa tcacaatcgc
     8581 ctgatgaata aggcccgaaa aatcgacagc gttttctgcc ttcagcgcac ctttccaggc
     8641 ttttaataac ggggccatca acttgatacg tttactgaac agatcgcgaa tctcttcggg
     8701 tgcactggca atcatttctg cctgtgcacc accgtgcatc cgcatcagac ttacccagcg
     8761 atcgaggcga gaggcaaggc gacgctgtaa tttttcatca tcccagaagt taccttctgg
     8821 cactgaccac tgcatttctt ccgtcagcca ttgccgccag cccttcgcct gcgctttctt
     8881 ttcgctgcat tgcttgcgcc actcagcaat aaagagttca tgacgggcag cggtatcatt
     8941 ttccagtttg ctgactatcg gaactttttt gctgccctgc tgaataatat gcagcgccag
     9001 cgcatgaaac gtgcgtgcgg taatgtcttc ggtatgtagc cgttcgcgaa tccgctcgtc
     9061 catctcttca gcggctttgc gaccaaacgc cagcaataaa atttgctcag gggacgcttc
     9121 accacgcgcc agcaaccagc ctgcacgggc caccagcacc gacgtttttc cgcttcctgc
     9181 acctgccagc actaacagag aatgctcgcc attaacgact gcccgggcct gcgccggatt
     9241 cagcggtgaa gactcgacct ggcggaaaaa atccgcatac tcggtaagca tggcttcggt
     9301 atacgcctgg ttatgctgca accgagcgct ttcaatatct ttcaaccagg cctgacattt
     9361 acgccacgcc tcacggcagt tatcgaattc ttccagtcgg ttaaccggca acggcaacgc
     9421 cgacaaagcc tggcggattt gttgctgcac gccagaggtt tgctcacgcg tcagccattt
     9481 attttcccca gtgcgcgtgg caatcaaatc cagttgctgg cgtaaaacac cagacgcaat
     9541 ttcgctcatc tcgccactcc accgccgcca gtgagcatca agatgatggt aaaaacgctg
     9601 cgtctcgccc cattcggtgc cgtgcagacg caccactttt tcgtccggca gtacaaattc
     9661 cagctcgccc cataccagac cgcgcttaca gtgaatcgcc agtaattgat tgaaaggaat
     9721 aaggtattcg tggcgatcgc cggagacttt aatcccggca ttgaggatca ccgcccgatc
     9781 gtaagggtgc tgtgccagac gttttccaag cgttgtcgct ttcagttcca tgactctgta
     9841 gatccacacg aaattatttc ttatcagtgt aaccgtcaga gaaaacgtgc tccagcccaa
     9901 aaaaacgtta caattgccgc ccattactga aaaccacagt aaagcgaggt tttatgcgta
     9961 ccgttttgaa cattctgaac tttgtgcttg gcggatttgc caccactctg ggctggctgt
    10021 tggcgactct ggtcagtatt gtgctgattt ttaccttacc gctgacacga tcctgctggg
    10081 agatcactaa actgtctctg gtgccttatg gcaatgaagc tattcatgtc gatgaactga
    10141 acccggctgg caaaaatgta ctgctgaata ctggcggtac ggtattgaat attttctggc
    10201 tgattttctt tggctggtgg ttatgcctga tgcacattgc aacgggcatc gcacaatgta
    10261 tttcaatcat tggcattcct gtcggcattg cgaactttaa aattgccgct attgcactat
    10321 ggccggttgg tcgtcgcgtg gtatcggtag aaacagcgca agctgcgcgt gaagccaatg
    10381 cacgtcgtcg ttttgaataa tccactaata tggcctttat gctaagtcct ttgctcaaac
    10441 gctatacctg gaacagcgcc tggctgtatt acgcgcgtat ttttattgcg ctttgtggaa
    10501 ccacagcgtt tccgtggtgg ctgggtgatg taaaactgac gattccgcta acgctgggga
    10561 tggtggcagc ggcgctgacc gatctcgatg accgactggc gggacgtttg cgtaacctca
    10621 tcattacgct gttctgcttt tttatcgcct cggcctcagt agaattgctg tttccctggc
    10681 cctggctatt tgcgattggc ttaacgctct ctaccagcgg cttcattttg ctcggcggtc
    10741 tgggtcaacg ctatgcaaca attgccttcg gtgcattgct gatcgccatt tacactatgt
    10801 tgggaacatc actgtatgag cactggtatc agcagccgat gtatctgctg gccggtgccg
    10861 tctggtacaa cgtcctgaca cttattggtc atctgctgtt cccggtccgc ccgctgcagg
    10921 acaacctggc gcgttgctat gaacaactgg cgcgttatct tgagctcaag tcgcgcatgt
    10981 ttgatcctga tattgaagat gaaagccagg caccgctgta cgatttggct ctcgccaacg
    11041 gtctgctgat ggcgacattg aatcagacga aactctcgct gctgacccgc ttacgtggcg
    11101 atcgtggtca acggggaacg cgtcgcacgc tgcattatta ctttgtcgca caggatattc
    11161 acgagcgtgc cagctcttct catattcagt atcaaacatt gcgtgaacat tttcgccaca
    11221 gcgacgtgct gttccgtttt cagcggctga tgtcgatgca gggccaggcg tgccagcaac
    11281 tgtcacgctg tattttgttg cgtcagcctt atcaacatga tccgcatttt gagcgcgctt
    11341 ttacgcatat tgatgctgcg ctggagcgga tgcgcgataa cggcgcaccc gccgatttac
    11401 tcaaaacact gggatttttg ctgaacaatt tacgcgccat tgatgcccaa ctggcaacaa
    11461 ttgaatcaga acaggcccag gcactacccc ataataatga cgaaaatgag ctcgctgatg
    11521 acagcccgca cgggttgagt gatatctggc tgcgtcttag ccgtcacttc acgccggaat
    11581 ccgccctctt ccgtcatgcg gtaagaatgt cgctggtgtt gtgcttcggc tacgccatca
    11641 ttcaaataac cggaatgcat cacgggtatt ggatcttgct gacaagtttg tttgtctgcc
    11701 agccaaacta taacgccacg cgccaccgcc tgaagttaag gattattggt acgctggtag
    11761 gtatcgccat tggcattcct gtgctgtggt ttgtgccatc actggaaggg cagctggtgc
    11821 tgctggttat taccggcgtg ctcttttttg ccttccgtaa cgtgcaatac gctcatgcaa
    11881 cgatgttcat cacacttttg gtgctactgt gttttaactt actgggtgaa ggttttgaag
    11941 tagcgttacc tcgcgtaatc gatacgctga ttggttgtgc cattgcgtgg gcggcagtga
    12001 gctacatctg gcctgactgg cagtttcgca atctgccgcg catgctcgaa cgcgccacag
    12061 aggccaactg tcggtatctc gatgccatac tggagcaata ccatcagggg cgtgataacc
    12121 gtctggcgta tcgtattgcc cgccgcgatg cacacaaccg tgatgctgag ctggcgtcgg
    12181 tggtatcaaa tatgtccagc gagccgaacg ttaccccgca aattcgcgaa gccgcgtttc
    12241 ggttgctgtg ccttaaccat acgtttacca gctatatctc agccctcggt gctcaccggg
    12301 agcagttaac taatcctgaa attctggcgt ttcttgatga cgcagtttgc tatgttgatg
    12361 acgcgttaca tcatcaacct gctgatgaag aacgcgtcaa tgaggcatta gctagcctga
    12421 aacagcggat gcagcaactt gaaccacggg cagacagcaa agaacctctg gtcgtacaac
    12481 aagttggatt attgattgca ttactgcctg agattggtcg tctgcaacgc cagattactc
    12541 aagttccgca ggaaactcct gtttcggcgt aagagagtca gcccattctg caagctcctg
    12601 gcggcgtgcc accgggagcg cagcttcatg aatgccgata atggcacctt caagcataaa
    12661 cagaatcttt tctgtcacca gactgttttg ctgccgcagt cgcaaccaac acatttttgc
    12721 cccaagtata cgtaacgccc gtacatcctt aatccccacc tccccgagaa tcgcttccag
    12781 atgaaaagac atattgggca aatctttcag tctttcccgg gtattgcgcg tgcttttctc
    12841 tttcagcgct gcatcgagag aatatttcga cagacgcacc agcttcagtt gatttcgcca
    12901 tagactttca tcaacccgat agtaattgag ggtaacggat cggccacact ttttatatgt
    12961 cagccagaca ggcggatgtt ttacacagta ctgtgcactt tgctcacaag cccgaagata
    13021 caactcacca tcagaaacca tcgcaaacac cgtgtcgtca acggtcaggc tgtaactgcc
    13081 aaacaatgat cggtattcaa ttgtgcccaa cgttgccagg tattcttgtg atttatagat
    13141 ccgcttatag gagaggcttt tcataaaatt ccttttaaaa tcataacata aaagaatgat
    13201 tcacattaac ggatccgtta actacgaaaa taggcaactt attcttaagg ggcaagatta
    13261 atttatgttt tcccgtcacc aacgacaaaa tttgcgaggc tctttccgaa aatagggttg
    13321 atctttgttg tcactggatg tactgtacat ccatacagta actcacaggg gctggattga
    13381 ttatgtacac ttcaggctat gcacatcgtt cttcgtcgtt ctcatccgca gcaagtaaaa
    13441 ttgcgcgtgt ctctacggaa aacactacag ccgggcttat cagtgaagtt gtctatcgcg
    13501 aagatcagcc catgatgacg caacttctac tgttgccatt gttacagcaa ctcggtcagc
    13561 aatcgcgctg gcaactctgg ttaacaccgc aacaaaaact gagtcgggaa tgggttcagg
    13621 catctgggct acccttaacg aaagtaatgc agattagcca gctctcccct tgccacactg
    13681 tggagtcaat ggttcgcgct ttacgcacgg gcaattacag tgtggtgatc ggttggttgg
    13741 cagatgattt gactgaagaa gagcatgctg aacttgttga tgcggcaaat gaaggtaacg
    13801 ctatggggtt tattatgcgt ccggtaagcg catcctctca cgccacgaga caactttccg
    13861 ggctaaaaat tcactctaat ttgtatcatt aagtaaattt aggattaatc ctggaacttt
    13921 ttttgtcgcc cagccaatgc tttcagtcgt gactaatttt ccttgcggag gcttgtctga
    13981 agcggtttcc gcgattttct tctgtaaatt gtcgctgaca aaaaagatta aacatacctt
    14041 atacaagact tttttttcat atgcctgacg gagttcacac ttgtaagttt tcaactacgt
    14101 tgtagacttt acatcgccag gggtgctcgg cataagccga agatatcggt agagttaata
    14161 ttgagcagat cccccggtga aggatttaac cgtgttatct cgttggagat attcatggcg
    14221 tattttggat gataacgagg cgcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc
    14281 actggctggt ttcgctaccg tagcgcaggc cgctccgaaa gataacacct ggtacactgg
    14341 tgctaaactg ggctggtccc agtaccatga cactggtttc atcaacaaca atggcccgac
    14401 ccatgaaaac caactgggcg ctggtgcttt tggtggttac caggttaacc cgtatgttgg
    14461 ctttgaaatg ggttacgact ggttaggtcg tatgccgtac aaaggcagcg ttgaaaacgg
    14521 tgcatacaaa gctcagggcg ttcaactgac cgctaaactg ggttacccaa tcactgacga
    14581 cctggacatc tacactcgtc tgggtggtat ggtatggcgt gcagacacta aatccaacgt
    14641 ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc
    14701 gatcactcct gaaatcgcta cccgtctgga ataccagtgg accaacaaca tcggtgacgc
    14761 acacaccatc ggcactcgtc cggacaacgg catgctgagc ctgggtgttt cctaccgttt
    14821 cggtcagggc gaagcagctc cagtagttgc tccggctcca gctccggcac cggaagtaca
    14881 gaccaagcac ttcactctga agtctgacgt tctgttcaac ttcaacaaag caaccctgaa
    14941 accggaaggt caggctgctc tggatcagct gtacagccag ctgagcaacc tggatccgaa
    15001 agacggttcc gtagttgttc tgggttacac cgaccgcatc ggttctgacg cttacaacca
    15061 gggtctgtcc gagcgccgtg ctcagtctgt tgttgattac ctgatctcca aaggtatccc
    15121 ggcagacaag atctccgcac gtggtatggg cgaatccaac ccggttactg gcaacacctg
    15181 tgacaacgtg aaacagcgtg ctgcactgat cgactgcctg gctccggatc gtcgcgtaga
    15241 gatcgaagtt aaaggtatca aagacgttgt aactcagccg caagcttaag ttctcgtctg
    15301 gtagaaaaac cccgctgctg cggggttttt ttgcctttag taaattgaac tgactttcgt
    15361 cagttattcc ttacccagca atgcctgcag atcctgcttc agagaagaca ttttattcgc
    15421 gtatttctct ttgttttccg catcttcaat cagctgaaca atcgtttcag aaagcgtttt
    15481 accgcgacgc tgcgcaagac cagccagacg ttgccagaca ataaattcca gatcgatcga
    15541 ttttttgcgc gtatgctggt gttccgcatt aaaatggcgc tttcgtcttg cccgaatggt
    15601 ttgcttcatg cgattgacca gttccggatt catatgcttg tcaatccagc catttaccag
    15661 cacgggttca ttttcgagcg agagcaacac atcgacggct tcctgggcgg cactggcttc
    15721 tatgtaacgg gtgattaact ccccttcgcg atgcttcttc accagatact tccatttcca
    15781 accgctttca agattttcaa gttgttgata tttcatagcg atctcaatgt taccgtgtaa
    15841 ctctttacag aatatcagct ttttaggcca gtgaagaaaa gaatctccat cctgtgagct
    15901 ttaacgccca tgccaggagt attgtcggtg ctttctacgt gtgctgtagt gccggttacg
    15961 gtataatcgc ggctttgaca acagactaaa aaacatcaac tttgaccatt acgaaacttg
    16021 catggcgtga cctggttcct gataccgata gctatcagga aatatttgct cagccacatt
    16081 tgattgacga aaacgatcct ttattcagtg atactcaacc gcgactgcaa ttcgcactgg
    16141 agcagttgct gcatacgcga gcatcctcct cttttatgct ggcgaaggcc ccggaagagt
    16201 ctgagtatct gaatcttatt gccaatgccg cgcgtacgct acaaagcgat gcaggccaac
    16261 tggtgggcgg tcactatgag gtttccggcc actccatccg cttacgtcac gcagtgagtg
    16321 cagatgataa ttttgcgact ttaacgcaag ttgtcgctgc cgactgggta gaagcggagc
    16381 aactctttgg ctgcctgcgc cagtttaatg gcgacattac cctgcagcct ggtctggtgc
    16441 atcaggcaaa tggcggtatt ctcatcatct ctttgcgtac actgctggcg caacctctgc
    16501 tgtggatgcg gctgaaaaat atcgttaacc gcgagcgttt tgactgggtt gcgtttgatg
    16561 agtcgcgccc tctccccgtc tctgtgcctt cgatgccatt gaagctgaaa gtcattctgg
    16621 taggcgaacg cgaatcattg tctgatttcc aggagatgga gccagagctt tcagagcagg
    16681 ctatttatag cgaatttgaa gatactctgc agattgtcga tgcagagtca gtaacccagt
    16741 ggtgtcgctg ggtgacattt accgccagac ataatcactt acctgcaccg ggagcggatg
    16801 cctggccggt acttatccgc gaagcagcac gctacaccgg tgaacaagaa acacttccgc
    16861 ttagcccgca gtggatcctc cgccagtgta aagaggtcgc ctccctgtgt gatggcgaca
    16921 ccttctccgg cgagcagcta aacttaatgc tgcagcagcg tgaatggcgc gaaggtttcc
    16981 tcgctgaacg tatgcaggat gagatccttc aggagcaaat cctgattgaa accgaaggcg
    17041 aacgcatcgg gcaaattaac gccctttcgg tcattgaatt tccgggtcat ccacgcgctt
    17101 ttggcgaacc ttctcgcatt agctgcgttg tgcatattgg cgatggtgaa ttcaccgaca
    17161 tcgaacgcaa agcggagctt ggcggcaata tccatgcgaa agggatgatg atcatgcaag
    17221 cgttcctgat gtcggaacta cagcttgagc aacagatccc cttctcagca tcgctgacat
    17281 ttgagcagtc atacagtgaa gttgatggag atagtgcctc gatggctgaa ctctgcgccc
    17341 tgataagcgc cctcgccgat gtgccggtga atcagagtat cgctatcaca ggttcagtcg
    17401 atcagttcgg tcgcgcccag ccggtcggtg gtttaaatga gaaaatcgaa ggcttctttg
    17461 ctatttgcca gcaacgtgag ttaaccggga aacaaggtgt cattatcccc acagctaacg
    17521 ttcgccattt aagtcttcac agtgaactgg tgaaagcggt agaagaaggc aaattcacca
    17581 tctgggcagt agacgatgtg actgacgcac tgccgttatt attaaatctg gtgtgggatg
    17641 gcgaaggcca aacgacgctg atgcaaacca tccaggaacg tatcgcgcaa gcatcgcaac
    17701 aggaaggacg tcaccgtttt ccatggccat tacgttggct gaactggttt attccgaact
    17761 gatcggactt gttcagcgta cacgtgttag ctatcctgcg tgcttcaata aaataaggct
    17821 tacagagaac atggtagata aacgcgaatc ctatacaaaa gaagaccttc ttgcctctgg
    17881 tcgcggtgaa ctgtttggcg ctaaaggccc gcaattgcca gcaccgaaca tgctgatgat
    17941 ggaccgtgtg gtcaaaatga ccgaaacggg tggtaacttc gacaaagggt atgttgaagc
    18001 agaactggat atcaatccgg atctgtggtt cttcggatgc cactttattg gcgatccggt
    18061 tatgccggga tgcctgggcc tggacgcaat gtggcagctg gtagggttct acctcggctg
    18121 gctgggcggc gaaggtaaag gccgcgcgct gggcgttggc gaagtgaaat tcactggtca
    18181 ggtactgccg acagcgaaaa aagtgaccta ccgtattcac tttaaacgca ttgttaaccg
    18241 tcgtctgatt atgggcctgg cggatggcga agtgctggtt gatggtcgtc tgatctatac
    18301 cgccagcgac ctgaaagtcg gtctgttcca ggatacgtct gccttctgat tataatcaga
    18361 cagagtatca aaaggcgaaa cctccgcaat gcggaggttt ctttttaaag agacagaatc
    18421 aggccattac taccctgtcc gccatggctt ctcgccagcc tcccagccat tgtgaccttt
    18481 gattcagcgt ctgataggga cacatttctt ttgagcgtcc ggcgatgccg gcctgataac
    18541 cacgttgatg tgcccgttcc aggcgatctc gtttttgtct cttcatgcct cgtttccctc
    18601 atactggttt ctggtggaaa agaaaacagt gattactaaa tgtgcaatca cagttaagca
    18661 ataccgcgaa tcataactgc cgtcaatgcg caaaattcac gccactgtca tatttgtgag
    18721 ctacacgggt atgccggttg tacaaaaaat gtgaagagga acaacttttt ccgcacgtta
    18781 gagacaaaaa aaccgcctca ggcaacatac cggaggcggt tatatttaca aatctttata
    18841 attaaggtag acgctttatc tcttgcgcaa tagaagcggc ttcctgactc caggcactgg
    18901 ccagtacttt aaccatctca tcgtaaccat cctgagtttg caccccttcc agacggaacg
    18961 gacgtttgat cagttgtccc tggtggttca acagccactc accactgaca atgaccttgc
    19021 catcatagcg accgttaaac tccgtgacgg taacattgag cgtgtcctgg gcgcttccca
    19081 gaggctggga ggcaaccacc cagccgggca gttgcgtact caggttggca accagggtgt
    19141 tgcgcaactg ttgatccaac gggctggccc acaagttgtt attggcaatc acatacttca
    19201 catcactggt ttgataaacc acaccattcc ccgccagata gtcaggaaca gtgacctgct
    19261 ctacccataa cagacgattg ccctggctgg cggtactttg tgtaccgctc tgcaccacag
    19321 gtaactggta atagttttta ttaatttcgc cggagctgca tccggccagc cacagtgctg
    19381 caatcgtcac tagccacttt ttcattgttt cgccctcttc ggctctggat cttttttgtc
    19441 cttcgcttca aataccagcg cgttactctt ctcattgagc gttttcagca ccggttgcag
    19501 ttctcgcaac acctgatcaa ggcgctgcat atccgccacc atcttgttgt aggctgcgga
    19561 gccaggctgg aagccctgca tgctgcgatt caattcacgc aacgttgact gcatatccgt
    19621 cggcaactgc tgcatcgact ggctagcgag gatcttgttc atgctatcca gcgtcgtttg
    19681 caggtttttc attgtgcgct gactttcaga aagcgtactg gttgcctgtt caatcatcgg
    19741 attcaatggc agtttgttga tcttatccaa cgcttccatc agtcgttgct ggatttgcgc
    19801 caggccgccg ctaacggtcg ggataatctg ataaccatta aattcacgaa taccggttat
    19861 tgcaggcgta tttggataga aatcgagatc aacatacagt gcaccagtga ccaggtttcc
    19921 ggttttcagc gatccgcgta aaccacgttt caacaattcg ccaaggtgct caacaacatc
    19981 cgcattttcg ccaagctgca ttttcagccg ctctggctcg atacgaatca gtaccggaat
    20041 acggtaatca tcgttaaatg tctgacgcat attcggcgca aagaatggca ctttgcttac
    20101 ggtacccagg cgaatacccc ggaactctac cggagctccc ggttgcagac cgcgtaccga
    20161 atctttaaag aacatcagat aatcaatgtg atcggtgtac aacgaatcct gaatgctctt
    20221 ctgatcatca tacaaaacga aagctgtttt cggtgccact ggctgcccta aatccagacc
    20281 ttccggcaca tcaaagctga caccgccact cagcagcgtt gtcaatgagc ccatctccac
    20341 acgcatccct gctgacgtca gatcaaccgc aatgccacta tctttccaga agcgaacatt
    20401 gttggtcacc agtcggtcat aaggtgcatt gatgaacagt tgatagctga tattgcgttt
    20461 ttgtgtatcg aaggtgctgg tttcaaccga acctacccga tagccacgga acagcaccgg
    20521 atctcctggc gagagctgcc cggctttttt gctatcgaga atcacacgga tacctttcgc
    20581 atcaggcggg gccaacggtg gcgagtccag caaatcgtat ttatccattt tgctgccttt
    20641 cgcgcctggc tgcagttcga tataaacccc agacagcagg gttcccagac cgctaatccc
    20701 ttctcgacca atctgcggtt tcaccaccca aaagacggtg tctttatgca gcaatttttc
    20761 cataccggaa ttcagccgcg ctttgatttc aacgtgcgtc aaatcatcag ccagtgtggc
    20821 gctttcaacc acgccgacgt caacgctacg gcttttaatg gtggttttgc caccttcaat
    20881 tccttccgca ttcgcggtga tcagggtcac ttccggtccc tgatggctgt aatgataaaa
    20941 aagaacccag gccccaatga gcgccgtgac gataggaaat atccacacgg gagaccagtt
    21001 cttcactttc tggattttgg cttccccatt attagattcc atactgtcag gactcctcat
    21061 gctctgattc aggttgacga tcccacgaca aacgcgggtc aaacgtcata gcagaaaaca
    21121 ttgtcattat gacgactaaa gcaaacatta atgcacccat tgccggataa atactcatta
    21181 aacctcccat acgcaccagc gccgagagca ccgcgataac gaaaacgtca atcatcgacc
    21241 agcggcctac aaactcaaca acttcataaa tcaaatgcat tctttcactg tcgcgcttgc
    21301 catgcccttt ggcatcccaa cacagccacg cgatggcgat catctttaac gttggcacca
    21361 taatactggc cagaaagatc accgcagcga cgggataaga gccttcgctc cataacagaa
    21421 tgaccccagc gagaatcgtc gacggcatct tcgagcctaa taaatccgtc accatgatgg
    21481 gcaaaatatt agccggaaga tacagcatga tggacgttac aagcagcgcg agtgtccact
    21541 gcaggctgtt tctacgccga acgtaccctt tggtactaca acgcgggcac acgggttcat
    21601 cagcaggaag gattgccgta caacaggagc acgaacgcag cccctgacgt atccccgtga
    21661 cgcctggttt tagcggctgg cgcagttctg gcatcggggc gatgtcgtcc cataaccagc
    21721 gacgatcaac gcactgaaaa gcgcgcagtt gcaggacaca aaataaacac caggggagaa
    21781 agctgctgcc taccccaatg ctgccgtaag ccatcagttt aacgaaactg accagcacac
    21841 cagcgaggaa aatctccgcc attccccagg ttttgagttg aaaaagcacc cgtgccagtt
    21901 gctcttttaa acggaccggt aattccgcgc gattcaccag taacagaatg gttatcagac
    21961 aaaacgcggg aaccagttgc acaaacaaca ggaaaaaggt gccgaggctg gcgtagtcct
    22021 cagaaaaaag cacgccggga atttccagta atgtaatttc actggtaact cctgcaacgt
    22081 tcatattcac aaaaggaaac aagttggaca gcaacagcat gaacagtgca gccaacgcat
    22141 aggcggtggg acgctgccga ggggcatccc acgccacggt taacgttgtg ccacaccggg
    22201 gacatgccgc tttctgacca tgctcaaggc gcggtaacgc caccagcatg tcacactgcg
    22261 agcacaggat gtgcttcgcg gcatgatgat gttcgcacat tggtactcct taatgatgcg
    22321 ctattttaca gatgactaac aaagtgttgg ttttcatgcc tgataaaccc agttcatcag
    22381 gcaattttac cgttgtcagc tgtctctgtt taaatcgact attttgcgat cagccaccat
    22441 tttttaacgc ttcaagatac tcccagcgtt caaaggcttg ctccagctcc tgctctgcag
    22501 cagccatatc ggcgagcact ttttgtgtct gctcatgtgg ctgactgaag aaggaagcat
    22561 ccgccacctg cgtctgtagg gcttccagct tcgcctccag atcttcgagc aattgcggta
    22621 gctgctccag ttcgcgctgc aatttatagc ttagtttgct actgctgcgt tttacagttt
    22681 ctgcttttgc cgcagcggct tcttcggttt ttttcaccgc aggctgtttg agcgccacat
    22741 actgctcttg ctgaccacgg gcatcatgat aaccgccgac ataacgacca attttaccgc
    22801 cgccttcgaa gatccaacat tctgtaacgg tgttatcgac aaactgacga tcgtggctaa
    22861 ccagcaatac cgtgccctga tagctgtcga tcagttcttc cagcagttcc agcgtttcga
    22921 catcaagatc gttggtcggt tcgtcaagaa tcaataagtt gcttggtttg aggaacaaac
    22981 gcgccagcag caagcggttc cgctcaccgc cagaaagcgc acgtaccggc gtcatcgccc
    23041 gtttcggatg gaacagaaag tcctgcaaat agcccaatac gtggcgtggc ttgccgttaa
    23101 ccatcacctc ttgcttacct tcggcaaggt tatccatcac cgttttatcg ggatccagtt
    23161 ccgcgcggtg ctgatcgaaa taagccactt ccagtttggt gccaacgtga atacgcccgc
    23221 tgtccgcttg aagctgaccg agcatcagtt tcagcagcgt ggttttgccg cacccattcg
    23281 gaccaatcag ggcaattttg tcgccacgta gaacctgggc cgaaaaatct ttcaccagtt
    23341 gcttaccgtc aacctggtag caaacgtctt ccatttcgaa aacgatctta ccggagcggc
    23401 tggcctcttc cacctgcatc tttgcggtac ccatcacttc gcgacgttca ccacgctcgc
    23461 gacgcattgc tttcagggcg cgtacgcggc cttcattacg ggtacggcgt gctttgatcc
    23521 cctggcgaat ccacacttct tcctgcgcca gtttgcgatc gaactcggca ttttgtagtt
    23581 cttccacgcg cagggcttct tctttttcct gcaggtactg gtcgtaattc cccggatagg
    23641 tcaccagctt gccgcgatcg agatcaacaa tgcgcgtcgc catattgcgg ataaacgaac
    23701 ggtcgtggga gatgaaaata atcgtcccat tgaaggtttt aaggaaccct tccagccagt
    23761 cgatggtttc gatatccaga tggttagtcg gttcgtccag cagcaatacg cgcggattgc
    23821 tcaccagcgc gcgtcctaat gccgctttac gcaaccagcc gccggaaagc gacgacagcg
    23881 caacattagg atctaacccc agttgcgcca gcacttcgtt gatgcggttt tccagctgcc
    23941 acaagttgtg gtgatccagc tgttcctgaa ccttcgccag ttcgttgaga tttttctcgc
    24001 tcgggtcgtt catcaccagg cgcgaaatat cgtgatagcg tttcagatat tccgcttgtt
    24061 cttcaatgcc ttcggcaacg aaatcataaa cgctaccctc aacgttacgc ggcggatcct
    24121 gttgcagacg agctacaatc aaatcttgct cgtaaataat gcgaccgtca tccagccctt
    24181 gttcacggtt gaggattttc attaacgtcg atttgcctgc gccgttgcgg cctaccagac
    24241 aaacacgttc attatcttcg atatgcagtt ctgcgttatc gagaagcggc gcgtcgctga
    24301 acgacagcca ggcgccatgc atactgatta atgacattac tattcctttc aggctgcggt
    24361 aatcagccag cagttgtgga tctggcggtt acgggcgaaa tcctgggaga gcgttttttg
    24421 cgtaatttct tgtgctttca gtcccagttt tgccaggccg ccgagatcca tacggaagcc
    24481 acgtttgttg ttcgagaaca tgatcgtccc acctgcacgc agcagacgtt tcaaatcttt
    24541 catcagcgcc agatgatcgc gctgaacatc aaacgcatct tccattcgtt ttgagttaga
    24601 gaaggttggc ggatcgataa agatcagatc gaactgttca tttgcctcac gcagccacgc
    24661 caggcaatcg gcctgaatca ggcgatgcgc acgcccggtc aggccattca gacgcaggtt
    24721 gcgttctgcc cactccagat aagtacgcga catatccacg gtggtggtgc tgcgtgcacc
    24781 gcctaatccc gcatgcacgg tggcgctgcc ggtataagag aacaggttga ggaaatcttt
    24841 gcctttgctc atctggccga gcatacgacg ggcgatacgg tgatcgagaa acagaccggt
    24901 atcgaggtaa tccgtcaggt tcaccaacaa gtgagcgtta tattcggtaa cttcaagaaa
    24961 ctcgcccttc tcgcccagtt tctggtactg atttttgccc ttctggcgtt cacgggtttt
    25021 cagcaccagt ttgtttggcg caatccccag taccgaaatg gttgcagcga taatatcgaa
    25081 cagacgctga cgcgctttgt gagcatcaat agtttttggc ggcgcatact cctgcaccac
    25141 cacccagtcg gcataacggt caacggcaac gttatattcc ggtagatcgg cgtcatacag
    25201 gcggtaacat tcaattcctt cctggcgagc ccacttctcg aattttttga ggttcttacg
    25261 caggcggttg gcgtagtctt ccgctaccat cgccggtttg ctgtctggag tgctttcggc
    25321 aacgtggtaa tttttctgta cgcagtccag cgggccgttt ttcgccttgt actgtttgtc
    25381 tgcacgcagc tgcaagcagc ttagcagatc cggcgaggca ctaaacaaag agagattcca
    25441 gccaccaaac tggtttttca tgatccgacc cagcaggcta tgcagcgcaa tcagcgccgg
    25501 ttcgctgtcc agacgttccc cgtatggcgg gttgctcaac actgtgccgt acggcccttt
    25561 cggcagcgga ttggtcagtt gcgcgacatc tttcacctca aaggtgatca gttcaccaat
    25621 ccccgcaaga cgggcgttag tgcgtgcacg ttgaatcacc cgtgcgtcgc tgtccgaacc
    25681 gtaaaaatga gagctatact cagccaggcc tttacgggcg cgagtttgcg cttccgcttt
    25741 cacttcctgc cagatagctt catcatgctg cgcccagccg ctaaagcccc aacgcccacg
    25801 gtgcaagcct ggtgcgcgat cggtcgccag catcgccgct tcaatcagca acgtaccgga
    25861 accacacatc ggatcgagca gcggtgttcc tggctgccag ccggatcgca tcacaatagc
    25921 ggctgccagg gtttctttga tcggcgcaat accagcacga tcgcgatagc cacgcagatg
    25981 taaaccatca ccactcagat caagagcgat actggcggtt tctttatgca gccagacgtt
    26041 aacgcggata tccggcgcat cgcgatcaac atttggacgc ggcagatttt tccgcgtgaa
    26101 agcatcgacg atcgcgtctt tcactttcat cgcgccgtac tgactgttgc ggatggtgtc
    26161 attcaaacca ctgaagtgga cagcgaaggt cgcgccagga ttaaacatct ctgtccagtt
    26221 gatcgcctga acaccgagat agaggtctaa atcgctgtaa accttacact cgcccagcgg
    26281 caacataata cgcgaggcca ggcggctcca catcaggctc tggtaaacaa gccgtgtgtc
    26341 gcccttgaaa tggaccccac cctgaaccac ctggcattca acggccccca ggttttccag
    26401 ttcagttttt aacagctctt ccagcccacg ggccgtactg gcaaacagag aattcatatc
    26461 gtcactttta ctctaagagc ctaccgcagt aggcgtaatt gttgcagtca gtctggacat
    26521 ggacagcgcg gagaaaccgg agcgtacaca agtacgtgag gatttcgagc acttccctgg
    26581 gccaaactga caaataaaat agcctaacgg gacaggcgct aagaaaattg ttgcgcatta
    26641 tagctaataa gcgcgtcatg tcataaagtt gagggcttat tttcatttga ggaccgcacc
    26701 gtggcgacat taacccggct ttttattcat cctgttaaat cgatgcgcgg cattggtctt
    26761 acacataccc tggcagatgt gagtggtctg gccttcgatc gcatctttat gatcacggaa
    26821 cctgatggta cgtttattac tgctcgccag tttccgcaaa tggtacggtt tactccttca
    26881 cctgtgcatg atggcttgca tctcaccgca ccagatggca gtagcgcata tgttcgtttt
    26941 gctgatttcg ccacacaaga cgcaccaacc gaagtttggg gcacacattt taccgcgcga
    27001 attgcgccag gcgcgatcaa caaatggcta agtggatttt tctcccgcga agtgcaatta
    27061 cgctgggtgg ggccacaaat gacccggcgc gtgaaacgcc acaacactgt acctctgtca
    27121 tttgctgatg gctatcctta ccttcttgct aacgaagcct cgttacgtga tctccaacaa
    27181 cgttgtccgg ccagtgtaaa aatggagcaa ttccgcccca atctggtggt ttccggcgcg
    27241 tcagcctggg aagaagatcg ctggaaagtg attcgcattg gtgatgtggt gtttgatgtg
    27301 gttaaacctt gtagccgctg tattttcacc accgtcagcc cagaaaaagg gcaaaaacat
    27361 ccggcaggcg aaccattaaa aacattgcaa tctttccgca ctgcccagga taacggcgat
    27421 gtcgattttg gtcagaattt aattgcccgt aatagcggcg tgattcgcgt tggcgatgag
    27481 gtggaaattc tggcaacggc tccggcaaaa atttacggcg cagctgccgc tgatgatacc
    27541 gccaacatca cgcaacaacc ggacgcaaat gtagatattg actggcaggg acaggcattt
    27601 cgtggaaata accaacaggt gttgctggag caattagaaa atcagggaat tcgtatccct
    27661 tattcttgcc gcgcgggcat ttgtggaagt tgccgtgttc agcttttaga aggcgaagtc
    27721 acgccgctaa aaaaatcagc aatgggcgat gatggcacca ttctttgctg tagctgtgta
    27781 ccgaagactg cacttaagtt ggcgcgttag actgcctgtt cgaggctgaa gctgtcaaca
    27841 ttaacctgcg gtttcagcct gtcgttcatg attttaatgg cgtcgcccaa ctgcatcgcg
    27901 cgacccgcaa taacaacgca gggttgtgcc agcaggcata gtgcggcatt ttcgccagat
    27961 tcaacgacca gcaaattgac ctgctcgtga gtatcgctca accaaacgca ggccgcatcg
    28021 cccggcatgg gcacccacat ttcaccatgg gaaacaaaat gccagctttt cggcatttgc
    28081 ggtttgagat agcgaattgc taccagtgca ttcagcacca gttcggcttt ttgttcttta
    28141 gataaattaa aatcgcggca cttttcttca aaagagaaat agagcgcggc gtcgtcaacg
    28201 caaaagcctg cgggagaaaa ggcatctgga gtcaacatct tgcgcgcaaa acgtgagcga
    28261 aatagcatac cattggctaa atcgagcatc attcgatcgt gctcttcatc gtaataccaa
    28321 cgccagttat cgtctggttt aattcgcatc tgcctctccc ctaccctata aaaccgcagc
    28381 aacacaatcc attgtcgctt cgcttaataa cctcaacaat aaaagtacaa aatgtctaaa
    28441 taagcaacag cgatgaatat aaaacaacca gggctgaaaa taaagccctg attatcttat
    28501 aaagaggaat tcgaagaaat aattagatat gggtaacgat ttctttaatc agcggcggac
    28561 ctttaaaaat aaaaccagaa taaatttgca ccagtgaggc acccgcagca atcttttcac
    28621 gcgcagcgat aaccgagtcg atgccgccaa caccgatgat cggtaagcga ccgtttaatt
    28681 ccagtgacaa gcggcgaata atttcggtgc tttttaactg aagcggacga ccacttaagc
    28741 caccggtttg atcgcaattt ttcattccct gaacaagaga acgatcgagt gtggtattgg
    28801 ttgcaataac gccatcaata ttatggcgaa ctaaactatc ggcaacctgg atcaattctt
    28861 cttcagaaag atccggcgcg atcttcactg cgatcggcac atatttatgg tgcatcgctt
    28921 gcaaatcatt ttgcttattt ttaatcgcgg ttaagagatc atccagcgct tcaccatatt
    28981 gcagcgtgcg taatcctggg gtattcggcg atgaaatatt gatggcgata tatcccgcat
    29041 aggcatagat tttttccata caaatcagat agtcatcttt gccctgctcc actggcgtat
    29101 ctttattttt gccgatgtta atacccagga cgccgtcata atgggccttt tttacgttct
    29161 ctacgaggtt atcaacgcca agattattaa agcccatacg gttgatcaaa ccttcggcat
    29221 ctaccagacg aaagagacgc ggcttgtcat tacctggctg tggacgtggc gtgacggtac
    29281 cgatctcgat cgatccaaat cccatcgcgc ctaacgcgtc aatgcactcc ccgtctttat
    29341 caagaccggc tgccagacca agcggatttt taaacgtcag gcccatgcag ttaacaggtt
    29401 tcgcaggcac tttctgccgc accagtgctt caaacggcgt tcctgtaata cggcgtaatt
    29461 gctgaaaagt aaactcatga gcgcgctctg gatcgagctg gaaaagggct ttacgaacga
    29521 aggggtagta catgaactct cctggattcc cggtgtgcaa accgggggcg tattatgtgc
    29581 gagttcggac caaaagggaa ttgacctgcg gcaaaaaaaa cgcaaacgtt ttccgaacct
    29641 gtttcagggc agattttgct ccttctttgc actcaacccg ccgtaatcat ttatcgcccg
    29701 ccaggcgaca tgacacggtg cgattgcctg acaccagctt gtctgacgct ggcttttagg
    29761 cggcacaact cccgcattac ttaacgcttt accatttacg ctcaactgaa tcaggttaat
    29821 gtagtaaggc gttgggttag tgagttgtac accctgcgaa ttccgtgtcc agcgtaattg
    29881 ttgataagct tccagcgggt cgcccggcaa accttcgggt cgccagaaca gtttaaatac
    29941 cgagcgcatc gccactttta cgctctgatt ttcaactttg ccggatggca cgctggcaat
    30001 gcttaactca aatagcgttt cgcgatccac aggcaagatg tcgctttccg ttctcagcaa
    30061 gcgcaacgtg cctgtagtac cgggcttcag gagaataagt ggcggagcgg ccagtaatgg
    30121 tgctggcact gtcgacgctt cccccccggc ccaacgcgtt gggcggttga ttttactgtt
    30181 aataagccag gattcctgac tggtattagt cagtaaaata gatatcgatt ctctgtctgc
    30241 cggaaagata aatcgcgttc cgccgaccac cactcctgcc cgcaattcac ccgccaccag
    30301 cagcgataaa acggcgaaaa tcaagagcgc caatctgttc cacgtattag tcataatgaa
    30361 gtacaaatgt cgcgctggca ttcactttac ccgactgcac atcgctattg acaggtcgca
    30421 attgcgcata aaaagttaat ggcaccttac cgctatgtag gctgtagtct ttactcgcct
    30481 ggttaagcgg aatcaatatc ttcgcatcat ccagaatcgc aattgccagc ccgctggctg
    30541 catccatacc actttcaatg gacagcattt cgctctgcac tccgtcgggg gttcctgaaa
    30601 aggtgacgtc aaccgtgctg acatctgttc cgcaatcctg caaattaatg atgaaagatt
    30661 tctccgggcc ataattcccg tgagaaaaac gtgtcagcga gacatcgcct aactgcacat
    30721 tgatttgctt gctatcctgt gccagcacgc atgtattacg acgaaacgat ccgctaatgt
    30781 tgaccgtgcc atcatgagca aataactcac tactccccgt taatcccagt aagcacagaa
    30841 tccagattat ccgtttcaac atcactcctt cactggtaaa ccagatcaaa atagagaacc
    30901 gccgtcgcat taccgcccgt tgctcccgcc tttgtggact tataacgaag ttgatatttg
    30961 agtgtgttat ccccggcttt taagggcgta agaggctgga cctgattgag cgggatttct
    31021 tgctgacttt gcgcatcaag aatttgcacg gcaatccccg ttgccatacc accgcttccg
    31081 cctgtgtcag ttagtttaag cagtgtcggc gcaagggtgt cagcctcgcc gctaaaaagg
    31141 acgtccgcgc cagtaatacc agcggcacaa ccggtgatat cgatattcaa atcagctgca
    31201 gtgcttacgg tattcgcggc ctgaaaatca ctgatattaa aatcgccaat atgaatattt
    31261 tgtaatgcac ttttggtggc gacatcacag ccctgatcga ccacattccc ataaatatgc
    31321 atactgactt gttcaggccc agccatcccg acatggatgt taagaaggct aaagaacaaa
    31381 ataacgtact gatatatcgt ttttttcttc atacgttcac gcgttaatca aaatttattc
    31441 tcaccgttgc cgtggcttca aatcgcccgg tttctggtgg tacgcctgtc gtgctgactg
    31501 gatagacatt cattcgcgcg atactgtctg catcatcagg aatagagagc ggtagtgtcc
    31561 caccagcagg ggaaataatg ttgttctggc tatcggtcac caccacgccc acgccaggac
    31621 gtgacgtctt aatcgcctgg ggatagctgg ggtctgaggt tgccgtcaga ctcaggttga
    31681 cgttaactaa cccttcgttt cccgtgttac acctgactgg aattgcgagt tctgccgttc
    31741 tggccccaag gggtttatgc cctacaacac gaaagttagc cgcaaatacg tcgcccagat
    31801 cgacctcgat agtctctccg gcattgacgg aacatgagcc aagtgaattg atcgtaccag
    31861 acaggtaata cactaaaacc ggtgtacctg tagtcgtgca ggaatcacct gcgggtatgt
    31921 tgtagcattc aaataatcgc gccagcgcca taggaggaat aacgaccgaa ccaacgaacg
    31981 gctgcaatat tttcagggaa aagctaccgc tattaccgct agccccctgg ccacctaaac
    32041 gttgaccgcc gggatcgcaa atatgccatc gtgtatctgc ccctgtgccc acgttttcga
    32101 aaggaaccgc agtaggagaa ctgcctttaa cctgcaataa aactttggcg gcaagatagt
    32161 cattaatttt gtaccagttg tcgcctaaat agactaacgg tgaatccgcg gcgaatgccc
    32221 atcggccact tctgacattg tctttatcgc aatcacagga aacataataa tcattcgatt
    32281 cgttccactg ataaaatttg tctttaacta ctccagccgt attttcttct tcctccaggg
    32341 ttttaacgcc aaaattgacg ccaatatacg gattcccctg agtggtatga cactgcccgc
    32401 tataagcagc agtttgagcg caccatagca ttaagacggc ggcaaaaaat agtcgtagta
    32461 ataacacgca tttttctcca aagattatct gcatatggca tccattcgcg taatcgcaga
    32521 gttaatttgt tgttcggtaa gctggtaact gatatgacaa atttgatcgg gagcttcgcc
    32581 ccagcgtaca gttatcgctc cctgagcggg caaaccggac atatagagcg agccatcatc
    32641 atcaataatg ccgctattag tctgtatgcc gtccagcgtc gctatcgcgc caaaacgcag
    32701 cggtataccc tgctttgatg ttttcattaa tacccttgct cccgcatggg cattgaactc
    32761 cgccagaacc aacgcccctt tggtgggaac cacgttgact actgcatttt caagatccac
    32821 gttacgtttt aacgatgccg catcaagtgc gattcgattt tcccgataag aagtggcata
    32881 ggggacaacg gtatatccgc gccagtcagt tttcagcccg gtgctgttcg ccacacgagt
    32941 attctctgcg cctggtgctg caatcagcac gttagtttcc cccagcgatt gccccagggt
    33001 aatgccctgt gaatgggcaa ctaaactgcc tgaaagagcg tagttcagtt gttgttgact
    33061 gccgttatcg ctgtagttgt agcccactcg ggcatctgca aacgcccctt tgtagtccat
    33121 gctggcgcta ccattagccg ttttcccctc gctgttatat ccctgttgca cgctgtagct
    33181 caggttaccg tcatcgagta gtgtctccgt taaacccagg ttttgtgtaa tgtgcccttc
    33241 gttatcgata ctggtgttag aggtcatcca ggcgtaattt tttttcctgg tatagctatc
    33301 attacttctg gggagcaaca gattgagcgg cagggaaaaa tttagtgcaa acacctgatc
    33361 agcatcaggt tgaccacggg acttactgta gttccaggaa acccccagcg agagatcttt
    33421 aatttgcgtg ttgtagccaa actgtaatag ccgatcctgt tgatcggtat gccagtaagt
    33481 ttgctggcta ccacttaaat aaaacgaacc gtactcgcct aattgctggg agatattgac
    33541 ctgcagtttg ccacgtttgg tgtaaaacaa attgtaataa cgcgaccaca tcggcgtatc
    33601 ttcatcatca ccgtcattaa attcgtagcc atccatatgt ttatacatgg tgtcggaaag
    33661 ggtgtagaaa cccgacgtcg aatagcgata gcccatcaat tgaaaattag tgcccaattg
    33721 gttgagcgtt ttggcataca gaaaacgata tgactgacct ttatgttcgc tttggtctgc
    33781 cagcgtactc ttcgcctggg tcgcatcgaa cgaaattgct ccgaaatcgc caaggttaaa
    33841 tcccagacca aacatggcgg cacggtaata ttcagcatat tgtccaccac cataccatgt
    33901 cgttccccac ggtccgcccc actgcaacgt ggcctgggca aatttgctct cctgctgttc
    33961 attactattg gttctgtatt tcgccagtgt caccgcgtat ttgattcgcc cctgacgctg
    34021 gagtaatggc acgctggaaa acggtacgct gtagctattg acgctgccgt ccgcttcttt
    34081 gatttcaact aacaaatcac cgctcgacga cgtggaatag agatcactaa tttcaaaagc
    34141 accaggcgat acataagttt ggtaaatggt gtaaccattt tgcttaatcg ttatctgggc
    34201 attgcttttg gcgataccac gcaccacagg cgcaaaccct ttcaggctgt ctggcagcat
    34261 gttgtcatct gacgccagct gcacaccacg aaagctgaca ctatcgaaaa aatcgcctgc
    34321 agtataatca tcacctacgg tcagttcgcc ttttagtgga ataaccgccc gctgcaaata
    34381 gctgctgaga ttcttccatt ctgcggtttg gccactactg cgactccatg ttgaattgtt
    34441 gcgcaatctc catgggccta aattaacgcc actgtttaaa ttcagaaaat agctgtcgcc
    34501 agaatcactg tctgcgctgc tatgaatact gttagccccg ctaaacgaat atcccagtaa
    34561 tagcgcgttg atcccttcat cccaacgttc aggggggaca taaccacggg cattactttt
    34621 gatggcgatt tgcggtactg aaatttccag acggagttta ttcacattaa acgttacgct
    34681 ggcatccgga ataatctccg ccaatggcac acacgcttgt ttgtccagtt gtttaaaagc
    34741 cggaaacgca tcagtattaa cacccattgc atccaggctt tcagtcgtca aacaggcggc
    34801 aatccccttc tctggcgtta tctcaacaaa attgacgttg cgcgtatcta caattgtctg
    34861 attcagaaca atatcgacac gataaacccc tggcgttatt ttttgtcctt tttcgaaacg
    34921 agacaaatcg gccaccattg agggatcatc tttgaaaaaa gcaggatcga accaggtttc
    34981 agcttgtgca ttgaacgctg ccgacacaaa caccaccagc ccaccaataa acgatggcac
    35041 tccaccagag cttaacaggc tgtgttgtcg atgagttcta tacatctcgc tctcttaact
    35101 gaagacggct tacgatttac agttgagttg cggcgtttcc gccccgtagt cattgatggt
    35161 tcgatacgtt acgctgctat ttttggcccc ctttggcaga gaaaattggt atttatcaaa
    35221 gggggaaatc atttgatttt taactacttt tccacctatt ttcaggtctg tcagggtaat
    35281 gaaataaggt gttgggttac tgaccgtaac catgtcagct gacgaagtgc aacgcagttt
    35341 cgccggagcc tcgcctaagg gatacgctaa accttttggt cgccagaaaa gtttgatgac
    35401 gctttgtgtg gcaatcttca gggtatttcc ctcttcccgc ttcgttgttg aagggactgc
    35461 ccgtacgcta gtgaagaaaa gggtttctct gtctttcgcc aacggcgcac caatgtacat
    35521 aatgcgtaac agattctcat tagccggatt cagcacaaat agcggtggtg taataataaa
    35581 atctgctgat tttttattat ctggggtcat cacccatgac tgaataagat aaggaacatc
    35641 cggactggtg ttacgtacac ccagtgaaat ttgctcttca ccttctgaat aaacaagacg
    35701 tgttgctgca agtccaatcc cgcctttacc tgccgcccat gcggatgagc agctgagtaa
    35761 tattgccgtt aaacttacgg tcacaattcc ctttgttatg caagttttca taaatagtca
    35821 ctcttaaaa
//
LOCUS       DAZFRJ010000026        35519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000026 DAZFRJ010000000
VERSION     DAZFRJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.026"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..1010)
                     /gene="astE"
                     /locus_tag="ACHKUN_001191"
     CDS             complement(42..1010)
                     /gene="astE"
                     /locus_tag="ACHKUN_001191"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO6659489.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1003..2346)
                     /gene="astB"
                     /locus_tag="ACHKUN_001192"
     CDS             complement(1003..2346)
                     /gene="astB"
                     /locus_tag="ACHKUN_001192"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO6659490.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2343..3821)
                     /gene="astD"
                     /locus_tag="ACHKUN_001193"
     CDS             complement(2343..3821)
                     /gene="astD"
                     /locus_tag="ACHKUN_001193"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO6659491.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3818..4852)
                     /gene="astA"
                     /locus_tag="ACHKUN_001194"
     CDS             complement(3818..4852)
                     /gene="astA"
                     /locus_tag="ACHKUN_001194"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO6659492.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4849..6069)
                     /gene="astC"
                     /locus_tag="ACHKUN_001195"
     CDS             complement(4849..6069)
                     /gene="astC"
                     /locus_tag="ACHKUN_001195"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO6659493.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            6515..7321
                     /gene="xthA"
                     /locus_tag="ACHKUN_001196"
     CDS             6515..7321
                     /gene="xthA"
                     /locus_tag="ACHKUN_001196"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO6659494.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7488..8198
                     /locus_tag="ACHKUN_001197"
     CDS             7488..8198
                     /locus_tag="ACHKUN_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6659495.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8203..8880
                     /gene="ydjY"
                     /locus_tag="ACHKUN_001198"
     CDS             8203..8880
                     /gene="ydjY"
                     /locus_tag="ACHKUN_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO6659496.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            8895..9602
                     /locus_tag="ACHKUN_001199"
     CDS             8895..9602
                     /locus_tag="ACHKUN_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6659497.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9602..10150
                     /locus_tag="ACHKUN_001200"
     CDS             9602..10150
                     /locus_tag="ACHKUN_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6659498.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10160..11326
                     /locus_tag="ACHKUN_001201"
     CDS             10160..11326
                     /locus_tag="ACHKUN_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6659499.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11299..12834
                     /locus_tag="ACHKUN_001202"
     CDS             11299..12834
                     /locus_tag="ACHKUN_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO6659500.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            12834..13487
                     /locus_tag="ACHKUN_001203"
     CDS             12834..13487
                     /locus_tag="ACHKUN_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6659501.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            13554..14861
                     /gene="ynjE"
                     /locus_tag="ACHKUN_001204"
     CDS             13554..14861
                     /gene="ynjE"
                     /locus_tag="ACHKUN_001204"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO6659502.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14870..15490)
                     /locus_tag="ACHKUN_001205"
     CDS             complement(14870..15490)
                     /locus_tag="ACHKUN_001205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6659503.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            15577..15984
                     /gene="nudG"
                     /locus_tag="ACHKUN_001206"
     CDS             15577..15984
                     /gene="nudG"
                     /locus_tag="ACHKUN_001206"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO6659504.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(15950..16222)
                     /locus_tag="ACHKUN_001207"
     CDS             complement(15950..16222)
                     /locus_tag="ACHKUN_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO6659505.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16458..17801
                     /gene="gdhA"
                     /locus_tag="ACHKUN_001208"
     CDS             16458..17801
                     /gene="gdhA"
                     /locus_tag="ACHKUN_001208"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO6659506.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(17918..18958)
                     /locus_tag="ACHKUN_001209"
     CDS             complement(17918..18958)
                     /locus_tag="ACHKUN_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6659507.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(19086..21047)
                     /gene="topB"
                     /locus_tag="ACHKUN_001210"
     CDS             complement(19086..21047)
                     /gene="topB"
                     /locus_tag="ACHKUN_001210"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6659508.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(21052..22095)
                     /gene="selD"
                     /locus_tag="ACHKUN_001211"
     CDS             complement(21052..22095)
                     /gene="selD"
                     /locus_tag="ACHKUN_001211"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO6659509.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(22212..22763)
                     /locus_tag="ACHKUN_001212"
     CDS             complement(22212..22763)
                     /locus_tag="ACHKUN_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO6659510.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22924..24780
                     /gene="sppA"
                     /locus_tag="ACHKUN_001213"
     CDS             22924..24780
                     /gene="sppA"
                     /locus_tag="ACHKUN_001213"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO6659511.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            24947..25963
                     /gene="ansA"
                     /locus_tag="ACHKUN_001214"
     CDS             24947..25963
                     /gene="ansA"
                     /locus_tag="ACHKUN_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO6659512.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            25974..26615
                     /gene="pncA"
                     /locus_tag="ACHKUN_001215"
     CDS             25974..26615
                     /gene="pncA"
                     /locus_tag="ACHKUN_001215"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO6659513.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(26776..27048)
                     /locus_tag="ACHKUN_001216"
     CDS             complement(26776..27048)
                     /locus_tag="ACHKUN_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO6659514.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(27090..27503)
                     /gene="msrB"
                     /locus_tag="ACHKUN_001217"
     CDS             complement(27090..27503)
                     /gene="msrB"
                     /locus_tag="ACHKUN_001217"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO6659515.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            27845..28840
                     /gene="gapA"
                     /locus_tag="ACHKUN_001218"
     CDS             27845..28840
                     /gene="gapA"
                     /locus_tag="ACHKUN_001218"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6659516.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            28924..29808
                     /locus_tag="ACHKUN_001219"
     CDS             28924..29808
                     /locus_tag="ACHKUN_001219"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO6659517.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(29859..30713)
                     /gene="yeaE"
                     /locus_tag="ACHKUN_001220"
     CDS             complement(29859..30713)
                     /gene="yeaE"
                     /locus_tag="ACHKUN_001220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO6659518.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(30803..31549)
                     /gene="mipA"
                     /locus_tag="ACHKUN_001221"
     CDS             complement(30803..31549)
                     /gene="mipA"
                     /locus_tag="ACHKUN_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO6659519.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            31985..33919
                     /gene="yeaG"
                     /locus_tag="ACHKUN_001222"
     CDS             31985..33919
                     /gene="yeaG"
                     /locus_tag="ACHKUN_001222"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO6659520.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            34032..35315
                     /locus_tag="ACHKUN_001223"
     CDS             34032..35315
                     /locus_tag="ACHKUN_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO6659521.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         8560 a         9410 c         8957 g         8592 t
ORIGIN      
        1 aagcaactta atacccgcag agtgatttct gcgggtaagt attagcttat tttttcgagc
       61 attaatcccg cgcgtaatcc caacgctacc aacggattag ggaataacac atactctaca
      121 tcatgggtta cggtaaaacg ttcctctccg tcctgcgcca gcaatgttcc tttctcaaac
      181 ggcataaaat tcagcgtgtc acttgccata tgcatttcga aggacggcga gtggcgagta
      241 atttgcgaaa ccacccgata acggagcggc ggtgttctca cgataccgac actctcacca
      301 gatagcagcg cagcaattgc gctggcagtt actgcaaact ggcgaagatc gttttgccca
      361 aagggcaacg ctttgccaag ttccagcgta caggccagcg cgccaaaatg tctggcgctg
      421 aaatgggtaa acgtaccacc aggttcctga tggaacacca gcgcctccag ccccgccgca
      481 cccagccacg tcagaaattt ctcgtcccag ggaatgtcgc gttgcggtaa tacaccgaac
      541 tgcggatgca aggagccacg aattgcggta tgtagatcaa ggtgccagcg cacagattct
      601 ttgccctggt cataaaaatc ttccaggcac tgttccagtt cgcgcgcccg acaggtttct
      661 ccgctttcag caaatagctg ccaacgaccg ccaaacattc gattcatatc gctatggcaa
      721 taacgtttcc cttgcttcag cgcaggagga ttcccgagga tcaccagcaa ccgccaacgt
      781 aacgggatct cgccgtgaga tatcgcgcca agcaacgcgt ccagcatctc cacaggtgcc
      841 gtctcattac cgtgtattcc cgctgaaatc accagtgcgc cttgcggtgg cgttaatggc
      901 gtcagttcca gcacaccatc gcccagccag cgccagcgaa cgccgttgat ttctcgctcg
      961 gtgataaccg gttttttacc cgttaaggtc agagcaagaa aattatccat tgccccctcc
     1021 ctcgcgctgg aacggataaa ccgaaccgag attcagtaat tggctcaata catccagtgc
     1081 ttcccgccct tcgcgcagca attgcgggtc ggccagatcg gcagcagtaa ggcgatcgcg
     1141 gtagtaacga tccacccagt cattgagcgc attaaacagc gtatcgttca tcatcaccgc
     1201 cggattcacc gcccggcgtt cttcttctgt caataccacc cgcaaccgca ggcacgccgg
     1261 gccgccgcca ttcgccatgc tttcacgtaa atcaaagact tttagttcgc taatcgggtt
     1321 gtcagcggca aggagttcat tgagataacc ccatactccg gcgtgttccc gacactcctg
     1381 aggcagcacc aacatcatag aaccatcatc gcggctcaac agttggctgt taaacagata
     1441 ggtagacacc gcatccgaca cggaaacctg agttgccgga acttctatcg ccataaaacc
     1501 gttcacccgc gcacgcaggt ttgccagtaa ctgtgactgg cgagcgaacg cctgttggtg
     1561 gcaaaacagc acctggcggt tactcacggc aatcacgtca ttatgaaaaa cgccctggtc
     1621 gataacgtcc gggttttgct gggcgaaaat cacctgttgg ggattcacct gattcagcct
     1681 tgccaccgcc tcgctggctt cgcgagtctg tcgcgccgga taacgggaag gccgggtatc
     1741 attgccttct tctcgcccgt agacaaaaag ttgcataccc ggctcaccgt aatgaccgcc
     1801 gagacgattg tggtttgccg ccccctcatc accgagcaac gctacctgcg gcaacgccga
     1861 atgaacgcta aatttctctt cgtcgttaaa aatcgctttt aaaagtgatt cggtgacgtg
     1921 cgcttccagc gagcggtgaa atttattgtt caggttggca acggtgagat gcactttgcc
     1981 atccagcgta tcggcagatg gcgcgatcgt tgccgcattg gctacccaca ttggcgaagc
     2041 ggaactgacg ctggaaagcc agtgcggtgc ctggcgtgca actttttcca gtacctgctc
     2101 atcgctacca ctgaatccca actgacgcag caccggaata aacggacgct cgtgcggcgg
     2161 gatcacggcc tgggggaatc ccgcatcggc aagggctttc attttcagta agccctgttt
     2221 tgccgcctgt cgcgggttag acacctgaaa acggtgacgg gtagaggctt cattaccaaa
     2281 cgacaggcca gcgtaatgat gcgtcagccc taccagcccg tcgaaattaa cttcccaggc
     2341 gttcatcgca ccacctcatc ggaaaaatcc agcccggggt taagcgttgc gggcaaagtt
     2401 aacgagtccg actccaggct cgccataggc catgcgcagt aatctgcggc ataccaggcg
     2461 ctggggcgat ggttaccgga tgcaccaatg ccgccgaatg gcgcggtact ggcagcacca
     2521 gtaagcggtt tgttccagtt aacaatcccc gcccgcgcct ccagcaacag ttgatcgaat
     2581 ttttcccgct cgggggaaac cagaccgcaa gagagtccga agcgagtgtt attcgccatt
     2641 agaatcgctt cctcgaaaga atcataacgc cagacgcgca ataacggtcc gaacacctct
     2701 tcatctggta cgccagcaac gcctgtcatt tcaatgatcc ccggcgtcag caacgatgtc
     2761 tctgattgta ataagcgcgg cgcaagcagg gttcgtccgc ccatcgcttc cagttgctgc
     2821 caggcagtaa ccacctgctg tgcggcctgt tcagaaatca gcccgccaat aaacggctgc
     2881 ggttcgtcat cccagttgcc cggcgttaat cgctggctga cggcaaccag acgagcaaga
     2941 aacgcatcgc cctgcgctcc gcttttgagc aataaacggc gggcgcaggt gcagcgttgc
     3001 ccggctgtga caaacgccga ctgaatggtc agatggacag ccgcgtcgat atccgccacc
     3061 tcatcgataa ttagcggatt attaccgccc atctcaaggg cgagaatttt ctccggctga
     3121 ccggagagct ggcgatgcag ctggtagccg gtattggcgc taccggtaaa cagcaaaccg
     3181 tcgagatcct ccagcgcact cagcgcctga cccgtttcac gcccgccctg caccaggttc
     3241 agtacgcccg gcggcaagcc agcctgctgc cataaacgca ttaccgcttc gccactccac
     3301 ggtgtcagtt cgctgggttt aaagataatg gtgttacctg ccagcaatgc cggaacgata
     3361 tgtccgttcg gcaaatgacc agggaaatta tacggcccaa acaccgccag cacgccgtgc
     3421 gggcgatgtc gcaggctcgc cgcaccgtcc ggcatttcac tacgctgctc gccggtacga
     3481 acgtgatacg ccttaattga tatcgcgatt ttattgatca tcgccgtcac ttcggttgcc
     3541 gcttcccagc gcggcttacc cgtttctctg gcaataatcg cggttaattc gcctttattc
     3601 ctttccagca atccggcaaa gcgttcgaca acgacctgac gttcagccaa tgagagccgc
     3661 gcccagcgcg gaaacgccgc acgggctgcc cgacaagcct gttcgacctg agcggcatcg
     3721 gcatcattgc cttgccataa cacctcgccc gataccggat tacgcttcac acgcgatgcg
     3781 ccctggcccg ttatccagtc accgttaatc cataaagtca tgctgttttc tcctctgcgc
     3841 acaggcgcac cagacgaacg cgatcccctg cgtggcattt gagggcatcc agttgtgcgg
     3901 cggttaaaat caaacgctcg gttgccggat cggtacgcgc cagcaccacg cggaaatggt
     3961 gataattttc attggcgacc aggcaggccg gaaaatcgcc ctgcgcaggc tgcccttctg
     4021 ccacttccac cagccgactt ttacgaatgg cgcgcacgcg gtcgatgtca cactcaagcg
     4081 ttggcccgcc gtcgaagatg tcgatatagt tgcggtaacg aaaaccttct ttctccagca
     4141 ccgcgcgggc aggcgcggtt tgcggatgta cctgaccgat gacgtcctgg gcttcctggg
     4201 ataaaaagtg ggtatagatc ggatgtttcg gcatcagttc tgcaataaat gccttttgcc
     4261 cggtgccgca gagaaaatcg gcgcggctaa aatccatcga aaagaagcgt ttaccgaggc
     4321 tttgccagaa cggtgaatag ccgtgttcgt caatcacccc gcgcatttca gcaaccactt
     4381 tgtcattaaa cttgtcgcga aaagccgcca taaacataaa gcgcgatttc gacagcaaat
     4441 agccgttgcc ctctttgcgc cagtccgggt cgagaaacag cgtgcacagc tcgctgctgc
     4501 cggtgtgatc gttactgaga aacagcgtcg gcaatgcgtt atagacattc agctcttttg
     4561 aggcgtgaac caacgtgccg acgcgatagt tgtaccaggg atcgttcagc ccaaccgcca
     4621 cctcaatggc acaaatcccc gccacggtgc ctgtctcgct atcttccagc acgaacacat
     4681 agccctgctc acttttgggc agttcgcctt gccaggtttt gattgccctt tcgatacgcg
     4741 ccgaaagcgt ggcttcattg gcgggaagcg acgtcaggcc gccgcccgtt ttgctggcaa
     4801 gctgcatcag cgccgagaca tctgatcgct caacgggacg gatgaccatc atgatgaacc
     4861 tcggctaaca aagtgttcgc aagcagctgc aaagcgatcc agtccggtcg tcacctcttc
     4921 ttcgctgaca ttgagcgcag gcgcaaaacg caccacgttg ccacccgcaa tcagtaccat
     4981 cacgcctgct ttcgccgctt cctgagagat ctgtttcgct tgcccggcgt aatcggcatt
     5041 cagtacacag ccaatcagca aacctaagcc gcgaacttca ctgaacaaac catagcggtg
     5101 attaatagta ttaagacgct caacaaacca gtcgtgacgc tgtttaacgc cattaagcat
     5161 ctctggtgtg ttgatgagct ccagcacttt gcctgccacc gccgaggcca gcggattacc
     5221 gccataggtg gtgccatgag tgccaacggt catcacgcgg gcgcactctt cggttgccaa
     5281 caacgcaccg accgggaaac cgccgcccag cgctttggcg gtagttaaca gatcaggcgt
     5341 cacgccgtag tgcatatagg catacagttc cccggtgcgc ccgacgccgg tttgtacttc
     5401 atcaaaaatc aacagcgcat tgtggcggtt acacaattca cgcagacctt gtaaaaacgc
     5461 gttgctggct ggcaccacac cgccttcccc ctggatgggt tcgacaatca ccgcacaggt
     5521 agagtcgtca atcagcgcgc tggcagagtt aatatcgtta tatgcagcat gacgaatatc
     5581 cgccggcagt ggcgcaaaat cctgtgaata ggctggctgc ccacccgcac tgacagtaaa
     5641 cagcgtgcga ccatgaaacg catttttgaa cgccacgatg ccgctcttat ggctgccgta
     5701 gcggtcgtga gcgaatttac gcgccagttt tagcgccgct tcgttggctt ccgcaccgga
     5761 gttacaaaag aagacgcgat cggcaaacgt ggcgtcgatc aattttttcg ccagtcgcag
     5821 taccggctcg ttggtgtaac cgttgccggt atgccagaac ttactcgcct gttcgttcag
     5881 cgcttcacgc agttccggat gcgcatggcc cagcgcgttc accgcaatgc cacccgcgaa
     5941 gtcgatatac tctttcccca gctgatccca caagcgcgaa ccttcgccac gtaccggtat
     6001 aaagggtgcc ggagcgtaaa caggtatcat ccattcatca aagttttcac gcgtaattgg
     6061 ctgagacata gcgacctcta cagtaaataa ttcgttattt atatgttaac aataagtaat
     6121 gtttgcgctg taaatgtaga ttgcagggtt cgtgccagcc agtgataaaa gtgcataaac
     6181 ggcggaggct aactggaaat caaggagtta taaccaaacc atatgcattt aaagtgcata
     6241 taaagtgaat acgtttgcga tgtgggtgaa taaaaagaat aaaaaacgca atgttatgca
     6301 gaagtaaaat ataattctgg aattgtgatc attgacgaaa tttactggaa attactgcgc
     6361 cattctgacg cagcgcgcac caaaagcggg cattttttgc gccatcgttg acatcattaa
     6421 caaccatcga tcaaatcact taacaacagg cggtaagcaa cgcgaaattc tgctaccatc
     6481 cacgcactct ttatctgaat aaatggcagc gactatgaaa tttgtctctt ttaatatcaa
     6541 cggcctgcgc gccagacctc accagcttga agccatcgtc gaaaagcacc aaccggatgt
     6601 gattggcctg caggagacaa aagttcatga cgatatgttt ccgctcgaag aggtggcgaa
     6661 gctcggctac aacgtgtttt atcacgggca gaaaggccat tatggcgtgg cgctgctgac
     6721 caaagagacg ccgattgccg tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg
     6781 gcggattatt atggcggaaa tcccctcact gctgggtaat gtcaccgtga tcaacggtta
     6841 cttcccgcag ggtgaaagcc gcgaccatcc gataaaattc ccggcaaaag cgcagtttta
     6901 tcagaatctg caaaactacc tggaaaccga actcaaacgt gataatccgg tactgattat
     6961 gggcgatatg aatatcagcc ctacagatct ggatatcggc attggcgaag aaaaccgtaa
     7021 gcgctggctg cgtaccggta aatgctcttt cctgccggaa gagcgcgaat ggatggacag
     7081 gctgatgagc tgggggttgg tcgatacctt ccgccatgct aatccgcaaa cagcagatcg
     7141 tttctcatgg tttgattacc gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga
     7201 cctgctgctc gccagccaac cgctggcaga atgttgcgta gaaaccggca tcgactatga
     7261 aattcgcagc atggaaaaac cgtccgatca cgcccccgtc tgggcgacct tccgccgcta
     7321 atttagcagc tctcctggct caaactgggt caggagaatt aaccttgaga aaaatcaaca
     7381 aactgtcagt aatgatttgt tgcctgccgt cctttgttat accgtcactg cgtttttagt
     7441 tgtctgacca cttctctatt atcaagtttg atataggaaa ctccacgatg aacgctgagc
     7501 gtaactttct gtttgcctgt cttatttttg cgctggtcat ttacgctatc cacgctttcg
     7561 gtttattcga tctgctcacc gatttacccc acttacagac actcattcgc cagagcggat
     7621 tgttcggtta tagcctctat attctgttat tcatcattgc caccctcttt ctgttaccag
     7681 gaagcatact ggtgatcgct ggaggaatag tttttggccc gctcttaggg acactactct
     7741 cattaattgc cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac
     7801 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
     7861 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
     7921 atattcaaaa ttacgcttac ggattaacca ccatcgcctt ctggccttat acccttattt
     7981 cggcactcac gaccctgctc ggtattgtta tttataccgt gatggcaagc gatctcgcca
     8041 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
     8101 ttctcgtcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
     8161 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
     8221 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
     8281 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
     8341 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
     8401 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
     8461 tcgctgttta tggcttatgc gaccccgaaa gcattttatg aagccctgaa agaggcaggt
     8521 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
     8581 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
     8641 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
     8701 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
     8761 aatgcaacat acacttatgg tgcggtggaa aaacgcggcg aagtgaaatt caaaggcaat
     8821 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
     8881 agccaggata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
     8941 tcatcatcct gttgttcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
     9001 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
     9061 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
     9121 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
     9181 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
     9241 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
     9301 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
     9361 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
     9421 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcgg
     9481 gcagcatgtt aacaggcggt actttctggt ttgtcaccgg actgtttatt ctgtttgccc
     9541 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgcct
     9601 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
     9661 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
     9721 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
     9781 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
     9841 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
     9901 ggctgactgg cggcaaagcc cgctcttcag cgatgaagaa cggctggcgc tggagtacgc
     9961 cgaagccgca agcgtaacgc cgccaacggt cgatgatgcc ctgcgcaccc gactggctgc
    10021 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    10081 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    10141 acgttcttaa ggagagaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    10201 ttctctggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    10261 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    10321 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctgg cggatgccgc
    10381 agacgcggtg aagcgcattc agaccgaagc tgctgccgga cgtaaaacgg gcggctcggt
    10441 ggatctgctc tgggtgaacg gcgaaaactt ccgcacctta aaagaggcca atttattaca
    10501 aacgggctgg gcggagactc tgcccaactg gcgctatgtc gacacacagc tgccggtgcg
    10561 ggaagatttt tcagtgccta cagaaggggc tgaatcgccc tggggcggcg cacaactgac
    10621 gtttatcgcc cgccgcgatg ttacgccaca gccaccacaa acgccgcaag ccttactgga
    10681 gtttgctaaa gccaatcccg gcacggttac ctatccgcgc ccaccggact ttaccggcac
    10741 ggcgtttctt gaacagttgc tgattatgct gacgcccgat cccgccgcgt taaaagttgc
    10801 cccggataaa gccactttcg cccgcgtcac tgcgcccttg tggcaatatc ttgacgcgct
    10861 acatccctat ttatggcgca aaggaaagga tttcccgcct tcgcccgcgc ggatggatgc
    10921 tctgctgaaa gccggcacat tgcgcctgtc gctgaccttt aaccctgctc atgcccagca
    10981 aaaaatcgcc agcggcgatt tgcctgcaag cagttacagt tttggtttta accaggggat
    11041 gattggcaac gtgcatttcg tcaccattcc tgctaacgca aatgccagtg ctgcggcgaa
    11101 ggtggttgcc aacttcctgc tctcacccaa tgcgcaactg cgtaaagcgg atcccgctgt
    11161 ctggggcgat ccttctgttc tcgatccgca aaaactgcct gatgggcagc gcgaaacatt
    11221 gcaatcaaga atgccgcaag atctgccgcc ggtactggct gaaccgcacg caggttgggt
    11281 aaatgcgctg gaacaagaat ggctacaccg ttacggtacg cattaatctt tttgctgtgg
    11341 gcgatggtgg cggtgattta tgcaccactg atcccggcag ctctcacgct gatatcgcct
    11401 gctttgtcgt tgacacactg gcaggcgtta tttgccgatc cacagttacc gcaggcatta
    11461 ctggcaacgc tggtgtcgac aaccatcgcg gcggtcgggg cgttgttgat tgccctgctg
    11521 gtgattgtgg cgctgtggcc ttggccgaaa tggcagcgta tgtgtgcccg tctgccgtgg
    11581 ctgctcgcca ttccccatgt ggcttttgcc accagcgccc ttctgctctt tgctgacgga
    11641 gggctgcttt atgactattt cccgtatttc actccgctaa tggacaaatt gggtattggg
    11701 ctgggcctca cccttgcagt gaaagaaagc gcatttctgc tgtggatctt agcggcagta
    11761 ttgagcgaaa aacggctgtt gcagcaggtc attgtgctgg attcgctggg ctacagccgc
    11821 tggcaatgcc tgaactggct gctcttgccc tccgtcgcac ctgcactggc aatggcgatg
    11881 ttggcgattg ttgcctggtc gctttcggtc gtggatgtgg cgattattct tgggccaggt
    11941 aatccaccga cgctggcagt aattagctgg cagtggttaa ctcagggcga cgcggatcaa
    12001 caaacgaaag gcgcactcgc cagtctgctg ctgatgctgt tactcgccgc ctacgttttg
    12061 ctgagctatc tgctatggcg cagctggcgg cgcactattc cccgcgtaga tggcattcgc
    12121 aagcctgcca cacctttatt accaggcact acgctggcga gttttttacc cttaaccggt
    12181 gtgctgtgtg tggttctgct ggcgatcctc gcggatcagt cgacgatcaa tagtgaagca
    12241 ctcatcaaca gcctgacaat ggggctggtg gcgacattca tcgctttgct cctgttactg
    12301 ctgtggctgg aatgggggcc acagcgtcgc cagttgtggc tctggttgcc cactctattg
    12361 cccgctctgc cactggtggc gggccagtac acgctggcgc tatggctgaa cctggatgga
    12421 agctggacgg cggtggtctg ggggcatctg ctgtgggtaa tgccgtggat gctgtttatc
    12481 ctgcaacccg cctggcagcg cattgattca cggttaattt tgattgcgca aacactgagc
    12541 tggtcgcggg ccaaaatctt cttttacgtg aaatgtccgc ttatgctacg ccctacgctg
    12601 attgccttcg cggtgggatt ttcagtcagt attgcgcagt atatgccgac gctgtggctg
    12661 ggcgcggggc gttttccgac gctcaccact gaggcggtgg cattaagcag cggcggcagc
    12721 aatggtattt tcgccgccca ggctttatgg caactgctat taccgcttat tatttttgcc
    12781 ctgaccgcgt tagtcgcaaa atgggtaggt tatgtcagac aaggactccg ctaatgctct
    12841 gcgtgaaaaa tgtttcgcta cgtttaccag aaagccgctt gctgacaaac gttaacttta
    12901 cggtggataa aggtgacatt gtcacgttaa tggggccgtc tggctgtgga aaatccactc
    12961 tgttttcatg gatgattggt gcactggctg gacagttttc ttgtacaggt gagctatggc
    13021 tcaatgagca acggattgac atgctaccca ccgcacagcg tcagattggc attcttttcc
    13081 aggatgcact gttatttgac cagttcagcg tcgggcaaaa tttactgctg gcgctaccgt
    13141 cgacgctcaa ggggactgcc aggcgcaatg ccgtgaaaga cgcgcttgat cgagcgggac
    13201 tggctgaaac ttatcaccag gaccctgcga cattatcagg cggtcagcga gcgcgggtgg
    13261 ccctgctgcg cgcccttctc gctcagccaa aagcgttact cctggatgag ccattcagcc
    13321 gtcttgatgt ggctctgcgc gataattttc gccagtgggt gttcagcgaa gttcgcgaac
    13381 tggcgatccc cgtcgttcag gtaacgcacg atctccagga tgttcctgct gatagttctg
    13441 ttctggatat ggagcagtgg tcagaaaatt acaacaaact gcgataacgc aaagtttttc
    13501 tcaatgcgtc agttcagaat ggcgcactca aaactacaat gtcgggattt tcgatgaaac
    13561 gtgtttctca aatgaccgcg ctggcaatgg ctttagggct ggcttgcgct tcttcgtggg
    13621 ccgctgaact ggcgaagcct cttacacttg accagcttca acaacaaaat ggcaaagcga
    13681 tagatactcg ccccagcgcg ttttataacg gctggccaca aaccttaaat ggcccttctg
    13741 gtcatgaacc tgccgcctta aacctctctg ccagctggct cgacaaaatg agcaccgaac
    13801 agctcaacgc gtggatcaag caacataacc tgaaagccga tgccccggtg gcgctgtacg
    13861 gtaatgacaa agatgtcgac gccgtcaaaa cgcgactgca aaaagcaggt tttacgcata
    13921 tctccattct gagtgacgcg ctaagcgaac cttcccgtct acaaaaactg ccgcactttg
    13981 agcagctggt ttatccgcaa tggctgcacg acctgcaaca aggtaaagag gttacggcga
    14041 aacctgccgg tgactggaaa gtcattgaag cggcctgggg cgctcctaag ctttacctta
    14101 tcagccatat tcccggtgct gactacatcg ataccaacga agtggaaagc gaaccgctgt
    14161 ggaacaaagt ttctgatgaa caactaaaag cgatgctggc aaaacacggc attcgccatg
    14221 acaccacggt cattctgtac gggcgtgacg tatacgctgg agcgcgtgtg gcgcaaatta
    14281 tgctctacgc aggcgtgaaa gatgtgcgtc tgctggatgg cggctggcaa acctggtccg
    14341 acgcgggact gcctgttgag cgaggaacgc caccgaaagt gaaagcggaa ccggatttcg
    14401 gcgtgaagat cccggcacaa ccgcagttga tgctcgatat ggaacaggcc cgtggactgc
    14461 tgcatcgcca ggatgcatcg ctggtgagca ttcgttcgtg gccagaattt atcggtacga
    14521 ccagcggtta cagctatatt aaaccaaaag gtgaaatagc cggagcacgt tggggacacg
    14581 ctggtagcga ctcgacgcat atggaagatt ttcataaccc ggatggcacc atgcgcagcg
    14641 ccgatgatat taccgctatg tggaaagcgt ggaatatcaa accagagcag caagtttcat
    14701 tctactgcgg caccggctgg cgcgcgtccg aaacctttat gtacgcacgc gccatgggtt
    14761 ggaagaatgt ttccgtgtat gacggcggct ggtacgaatg gagcagcgat ccaaaaaatc
    14821 cggtagcaac cggtgaacgc ggcccggaca gtagcaaata acattgcaat tactgacgct
    14881 ggtgcgactt cagcgtcaga tacccactcc acacccgcgt aaaggtcgtc atccagcaca
    14941 acgcaccgaa gatccacgca aaccacgcaa accatgcagg gaataaacat cccagcacaa
    15001 acagtaagat cgtttctgtg ccttcggtta acccgcccag ataataaaac gatttatgcg
    15061 cataaccggg attatcaatc tgatgtttcg ccgccagcgc agcaaaagcg agaaaactgc
    15121 tgcctgtgcc gataaacgca aacagcagcc agccgcctgc cagggcgttt tgctgcggtg
    15181 cggcgaggat aaacccaaaa ggtaccagcg cgtaaaaaag aaaatcgaga gaaatatcaa
    15241 gaaagccgcc cgcgtcggta agccctctgc gccgcgccag cgcaccgtcc agaccatcaa
    15301 gcagcctgtt caacaaaatg acgacaagcg ccgccagata ccagcccagc gccagaaaag
    15361 gcagtgccag cacgccgatg gcaaatccca ctaatgttaa accatctggc gtaatgcccg
    15421 gtttatcaag aacccgcacg cactgatgca acaacggttt aatccgggga tgaagatggc
    15481 ggtctagcac aggcactcct taaatataaa gcctttctga ttgagcaaca gtgcggatat
    15541 tatggcattt ttcgcttatc tgcccgtgtg taatttatga aaatgattga agttgttgcc
    15601 gccatcattg aacgtgatgg caaaatttta ctcgcgcaac gccccgccca gagcgatcag
    15661 gcgggattat gggagtttgc cggtggtaaa gtcgagccgg atgaaagcca gcggcaggcg
    15721 ctggtgcgtg agttacgcga agaactgggc atcgaagcaa ctgtgggtga gtatgttacc
    15781 agccatcagc gagaagtttc gggccggatt atccatcttc atgcctggca cgtacccgac
    15841 ttccacggga cgttacaggc acatgaacat caggcgctgg tctggtgctc acctgaagag
    15901 gcgctgcaat atccgctggc ccctgctgac attccattat tagaggcgtt tatggcttta
    15961 cgcgccgcca gaccagcgga ttagtgctaa gggttttgtc atcccgctgg cattgcagca
    16021 gtattccctc ggctttaatt accgcgcctt cagaataatt tttatcctga taaacgcagc
    16081 actgagtaca gggctgcgct gactgcccgc ctgaactgaa cacttctggc ggtacgttta
    16141 cctccacgtc cggacgataa tgcgggttag ccagtgcaat taatggaaat gctaatacta
    16201 cggcgaacaa tgctcgactc acagggaact ccttaacgtt attgtctctg ctactgataa
    16261 cggtagccgg gtggcaaaac tttagcgtct gagttatcgc atttggttat gagattactc
    16321 tcgttattaa tttgctttcc tgggtcattt ttttcttgct taccgtcaca ttcttgatgg
    16381 tatagtcgaa aactgcaaaa gcacatcaca taaacaacat aagcacaatc gtattaatat
    16441 ataagggttt tatatctatg gatcagacat attctctgga gtcattcctc aaccatgtcc
    16501 aaaagcgcga cccgaatcaa accgagttcg cgcaagccgt tcgtgaagta atgaccacac
    16561 tctggccttt tcttgaacaa aatccaaaat atcgccagat gtcattactg gagcgtctgg
    16621 ttgaaccgga gcgcgtgatc cagtttcgcg tggtatgggt tgatgatcgc aatcaggtac
    16681 aggtcaaccg tgcatggcgt gtgcagttca gctctgccat cggcccgtac aaaggcggta
    16741 tgcgcttcca tccgtcagtt aacctctcca ttcttaaatt cctcggcttc gaacaaacct
    16801 tcaaaaatgc cctgactacc ctgccgatgg gcggtggtaa aggcggcagc gatttcgatc
    16861 cgaaaggaaa aagcgaaggt gaagtgatgc gtttttgcca ggcgctgatg actgaactgt
    16921 atcgccacct gggcgcggat accgacgttc cggcaggtga tatcggggtt ggtggtcgtg
    16981 aagtcggctt tatggcgggg atgatgaaaa agctctccaa caataccgcc tgcgtcttca
    17041 ccggtaaggg cctttcattt ggcggcagtc ttattcgccc ggaagctacc ggctacggtc
    17101 tggtttattt cacagaagca atgctaaaac gccacggtat gggttttgaa gggatgcgcg
    17161 tttccgtttc tggctccggc aacgtcgccc agtacgctat cgaaaaagcg atggaatttg
    17221 gtgctcgtgt gatcactgcg tcagactcca gcggcactgt agttgatgaa agcggattca
    17281 cgaaagagaa actggcacgt cttatcgaaa tcaaagccag ccgcgatggt cgagtggcag
    17341 attatgccaa agaatttggt ctggtctatc tcgaacgcca acagccgtgg tctgtaccgg
    17401 ttgatatcgc cctgccttgc gccacccaga acgaactgga tgttgacgcc gcgcatcagc
    17461 ttatcgccaa tggcgttaaa gccgtcgccg aaggggcaaa tatgccgacc accatcgaag
    17521 cgactgaact gttccagcag gcaggcgtac tgtttgcacc gggtaaagcg gctaatgctg
    17581 gcggtgtagc aacgtcaggc cttgaaatgg cgcaaaacgc tgcgcgcctg ggctggaaag
    17641 ccgagaaagt tgacgcacgt ttgcatcaca tcatgctgga tatccaccat gcctgtgttg
    17701 agcatggtgg tgaaggtgag caaaccaact acgtgcaggg cgcgaacatt gccggttttg
    17761 tgaaggttgc cgatgcgatg ctggcgcagg gtgtgattta agttgtaaat gcctgatggc
    17821 gctacgctta tcaggcctac aaatgggcac aattcattgc agttacgctc taatgtaggc
    17881 cgggcaagcg cagcgccccc ggcaaaattt caggcgttta tgagtattta acggatgatg
    17941 ctccccacgg aacatttctt atgggccaac ggcatttctt actgtagtgc tcccaaaact
    18001 gcttgtcgta acgataacac gcttcaagtt cagcatccgt taactttctg cgatagcagc
    18061 agatatgcca gtaaagaaac cccattagac tatttttttg ataatcttct tcgctttcga
    18121 acaactcgtg cgcctttcga gaagcaagca ttatataatg ccaggccagt tcttcttcaa
    18181 ttgtcccgtt ttgaaaagct gtgcttgata tcgaggtcat ccatgataat tccgccgccc
    18241 atattagctt cgccgaggat ttaccggagc gattattagc gcaatcagag atatagtctg
    18301 agggaaaaac agcaaattta ttcaacaagg cgataacctg ctctggggct tcctccatgt
    18361 ttgctttaaa ggtattggct ccatggtcgc cagaaagaaa atactccatt aaggcacaat
    18421 aactttcgct atcttcgata ccccattgat cctctaaaga ctcgcgtctt ttacttatga
    18481 tatcgatcga gtcaaaggga agcacatgat attggaaggt atctttgcca ggttcaggct
    18541 ttcgcggcca gaactccagc gtttcagacc attgcttatg atagaaccga taaggtgcga
    18601 tcaattgtag cgcctgtaac ttctcgatac tgagcggctc aataccttta gcctgataat
    18661 aatgcagttg ttcttttttt gctttaaaac cggcccgaac aataagcccc atcataatta
    18721 atagataaag aaaagagcat cccgcggtaa tcaggcctct ttcattcaaa ccgttggatg
    18781 ttatcgctgc gaacacaaac attacagcga caacacatgt taaataaaac ccccacttac
    18841 aaagcagcat ggccttattt tctttaatca tccgttcaaa attactatta aatatttccc
    18901 agccattaaa agaatacttc tcgctcccag gatggttttg taataaaact tttttcatca
    18961 cattccctgt tattacatac tttagaggcg ataattttat ccatgcaaaa aaaatatcca
    19021 acgaattttc ttgatccgct ggatatttca gaatatgact cgaatagcac gaaagattca
    19081 ctcgcttacg ctatcgcccc gcttccgact tcatctgctg gcggactttt tttcgcacta
    19141 cgtttacgcg gtgcagcctt tttcttatca gcactgccac cactgcccgg agccacaatg
    19201 ccgcgaaact gccgcaccgg cgtacgtttg gcttgatcaa taagctgata tagcgtcccc
    19261 accagcggct gcataaagtc ctgatagcga cactgctttt cgctgatttg cgtcagcacc
    19321 gattcccagt gcgcggtcat gtccggtcgc gtcgccattt ctggcagcga atggaataac
    19381 gcttttccgg cgtcggtaga gtggatatag cgcccttttt tggtcagaaa accacgcttg
    19441 aacagcagtt caataatccc ggcacgggtt gcttctgtcc ccagaccatc ggtcgcacga
    19501 aggatctttt tcagatcttt gtcctgcaca aagcgcgcga tcccggtcat cgccgaaagc
    19561 agtgttgcat cggtaaaatg gcgcggcggc tgggtttgcc gctctaccac ttcacctttt
    19621 tcacacagca actcatcgcc tttcgccacc acaggcaatg gcgtaccgtc gttttcttca
    19681 tcgcgctctt tgctgcctaa cagcgtgcgc cagcctgctt cagcaagaaa acgggcttta
    19741 gcgacaaatt tgcctttggc aatgtccagt tcgataacac acttgcggaa caccgcatcc
    19801 gggcagaact gcatcaggta ttgacgggca atcaggttat agaccttcgc ttcgttctcc
    19861 gtcaggttga tcgcagaact ccgtgcggtc gggatgatgg cgtggtgcgc atcgaccttt
    19921 ttgtcatccc aacagcggtt gcgtatatct ggatctacca ctgactgcgg caacagatcc
    19981 ggtgcatgaa cgctgatggc attcatcacc gcgtggcgtc cggcaaaatg ttcttctggc
    20041 aaatagcgac aatcggaacg cggataagtg attagcttgt gcgtttcgta cagcttctgg
    20101 cagatatcaa gcacgttctg cgcgctcaga ccgaagcgtt ttgccgcttc aatctgtaac
    20161 gccgaaagcg aaaacggcag cggcgcggat tctgattccc gtttatcgtt atagctggtg
    20221 acaatagccg gttgaccact aatgcggtta accacatgct ccgccagtgg acgatgtaac
    20281 aagcgccctt cttcatcctg gtacggttca cacgcttcgc tcggttgcca gatagcggta
    20341 aaccgctcat cggcaggtgt cacgatatgc gctttgactt caaagaagtc tttcgccacg
    20401 aagttttcaa tctcttcatc gcggcgcacc accagcccga gcacgggtgt ctgcacgcgt
    20461 cccacggaaa gtacgccctg ataaccggca ttgcgaccga gaatggtata cgcacgggtc
    20521 atattgatgc cgtacagcca gtcggcacgc gctcgcgcca gcgcagaaac gcacagcggt
    20581 acaaactcac tgttggaacg aagacggtcg atcgcccgct caaccgcctg cgggttcagg
    20641 tcgtttatca agcaacgctg cacctgctgg cgcttttccg gtgccagttg cagatagtcc
    20701 agcacttcat ccaccagcaa ttgcccttca cgatccgggt ccccggcgtg aacgatttcg
    20761 ctggcttcat gcaggaagcg tttgatgacg ttaagttgtt tggtcacgga gggtcggggc
    20821 tgtaattgcc acttttccgg gacaatcggc aaatccgcaa gattccagcg cgcatagcgg
    20881 ctgtcgtagg cgtctggctg cgcctgctca agcaggtgac cgatacacca ggtcaccacc
    20941 tgaccattac cgcactcgat aaagccatcg cctttacggt gcggtttggg caggacatca
    21001 gcaatggcgc gcgccagact cggtttttcg gcaataaaca accgcattga attaatggat
    21061 ctcaaccatg gcacgaccgc cgcgcgctgg caccagttcg ccaatcgccg tcagttcaat
    21121 gccaaactcg gcggctgtag ttttaacctc attttctgct tccggcatga ccgccagcag
    21181 caaaccgcca gaagtttgcg gatcgcacag cagatcgcgc acttcacgcg gcatttcacc
    21241 catcagatga ccgtagctgg caaagttacg ttcggtgccg ccaggcactg cgcccaactt
    21301 aatgtactct tcaacccccg gcaattttgg gatcgcgtca tagtcaacgc gcgcctgcac
    21361 acctgcgccc tgacacattt cgctcaaatg gcccagcaga ccaaaaccag taacgtcggt
    21421 catcgctttt acgccttcga tgttggcaaa ggacgcacct gcgatgttca tccggcacat
    21481 cacttccgtc gccagtccct gatgttctgg tttcaacagt gattttttct cagccgtggt
    21541 aagaacgccg atccccagcg gtttcgtcag gaacagtttg catccggctt gtgcggtact
    21601 gtttttcttc acccgctcgg tcggtacgat ccccgttacc gccagaccaa aaatcggctc
    21661 cggcgcatcg atggaatgac cgccagccag cgcaataccc gcctgacggc atgcatagcg
    21721 tccaccttcg gttacttcgc gggcaatttc tggggaaagt ttgttaatcg gccagccgag
    21781 gatcgcaatc gccataatcg gtttgccccc catcgcgaag atatcgctga tggcgttagt
    21841 cgccgcaatg cggccaaaat cgaaaggatt atcaacgatc ggcataaaga agtcggtggt
    21901 actgataacg ctggtgccat tgcccagatc gtacaccgcc gcatcgtcgc gggtctcatt
    21961 acccacaagc aaattcggat caacaaactt cgcctgctca ctgtgcagga tggtttccaa
    22021 cacttttggg gaaattttac agccgcaacc agctccgtgg ctgtattggg tcaaacgaat
    22081 cgagttctcg ctcatggaca tctcctgtca atgcaatccg ggtatggtaa ccctcattcc
    22141 gtgaagtgat aagtgagagt gtctgaattc ctgcgccttt gctcacaatc cagacagttt
    22201 cgcgacaatt atcagaaata agttacaaac ggcgtcgggt ccgggacgtt aatcgacgta
    22261 gatgctttca actgcggcgt gccgaggtag agaaaaccga caattttatc ctgctcacgg
    22321 caaccgaatg cttcacgcac taccggactt tcagttaatg cgccactgcg ccagatgccg
    22381 ccaaatcctt gggcaacggc tgccatttgc atcgccatga ccgcgcatcc ggcagacatt
    22441 tcctgttccc atcgcgggac tttatgattt tcttcgcatt tcgccaccac cgtgatgatg
    22501 agcggtgcgc ggaacggcgc attacgggct ttgtcgatag ctttgtcatc actaccggca
    22561 gcaatcgccc cctgttccag tacggcgctg aaacgctcgc gcccttcccc ttcaatcaca
    22621 aaaaaatgcc acggttgcat ggacttatgg tccggcgcac gcatacccgc acgcaggatg
    22681 ttttgcagtt gttcacccgt tggcgcgggt tcagccaggc gggaggcgct acggcgattg
    22741 atcaatagtt cgagtgcatc catttgatta actcctgtcg tgatatttat tcacaaaatt
    22801 aacacgagag tggattttgt tacagcacag tccgcaattc ctgctgacaa gtgccggttg
    22861 ggtcattacg ataaccacat ctattgcgcc tgtgacaggt gtgaccttaa gttgggagaa
    22921 tacatgcgaa ccctttggcg atttattgcc ggatttttta aatggacgtg gcgtctgctg
    22981 aatttcgtcc gtgaaatggt acttaacctg ttctttattt tcctcgtact ggttggtgtg
    23041 gggatctgga tgcaggtcag tggtggtgat tcgaaagaaa cggccagtcg tggcgcactg
    23101 ctgctggaca tttctggtgt gatcgtcgat aaacccgaca gttctcagcg gtttagtaaa
    23161 ttaagccgcc agctgcttgg tgccagttcc gatcgtctgc aggaaaactc actgtttgat
    23221 atcgtcaaca ccattcgcca ggcgaaggac gaccgcaata tcaccggtat cgtgatggat
    23281 ctgaaaaact tcgcaggcgg cgaccagccg tctatgcagt acatcggcaa agccctgaaa
    23341 gagtttcgtg acagcgggaa accggtttat gccgttggcg agaactacag ccaggggcaa
    23401 tattatctcg ccagtttcgc caataaaatt tggctgtcac cgcaaggcgt ggttgatctg
    23461 cacggttttg ctaccaacgg tctgtactac aaatcgttgc tggataagct gaaagtttcc
    23521 acccatgtgt tccgcgtggg tacgtataaa tctgccgttg aaccgtttat tcgtgatgat
    23581 atgtcaccgg cagcccgcga agctgacagc cgctggattg gtgagctgtg gcaaaactat
    23641 ctgaatactg ttgccgctaa ccggcagatc cctgctcagc aggtattccc tggcgcgcaa
    23701 gggttgcttg agggtttaac caaaaccggt ggcgataccg cgaaatatgc actggaaaac
    23761 aagctggtcg atgcactggc atcgagtgcc gaaatcgaaa aagctctgac caaagagttc
    23821 ggctggagta agactgataa aaattatcgc gccatcagtt attacgatta cgcattgaaa
    23881 acgccggcag ataccggtga cagcatcggt gtcgtcttcg ctaatggcgc aattatggat
    23941 ggcgaggaaa ctcaggggaa tgttggcggt gataccactg cggcacaaat ccgcgacgct
    24001 cgccttgacc cgaaagtgaa agcgattgtc ctgcgtgtta atagcccagg cggcagcgtt
    24061 accgcgtctg aagtgattcg cgctgaactg gcagcagccc gggcagcggg taagcctgtg
    24121 gttgtatcga tgggcggcat ggcggcatct ggtggttact ggatttccac gccagctaat
    24181 tacattgtgg ctaaccccag caccctgacc ggttctatcg gtatcttcgg cgtgatcacc
    24241 accgtagaaa atagtctgga ttcgattggt gttcatactg atggtgtctc aacttcaccg
    24301 ctggcggatg tttctatcac cagggcactg ccgccggaag cgcagcagat gatgcaatta
    24361 agcattgaga atggctataa acgctttatc acgctggttg ctgatgcgcg tcattcgacg
    24421 ccggagcaaa ttgataaaat cgcccagggc cacgtctgga ccggtcagga tgcaaaagct
    24481 aacgggctgg tcgatagtct cggggatttc gatgatgcgg ttgccaaagc agcagagctg
    24541 gcaaaagtga aacagtggca tctggaatac tacgttgatg aaccgacctt cttcgacaaa
    24601 gtgatggaca acatgtctgg ttctgtccgg gcaatgttgc cagatgcgtt ccaggccatg
    24661 ttacctgcac cgcttgcctc ggtagcctct accgttaaaa gtgaaagcga caagctggcc
    24721 gcgtttaacg acccacaaaa ccgttatgcg ttttgcctga cctgcgccaa cgtgcgttaa
    24781 gtcttgtact gagtggccga cagatcgtcg gccacattat tttttacctc gacgaatcct
    24841 cttcccgctg tctcgaccca tatttcctta tccgcatagt atcaggtgcg ctcctcctgc
    24901 ctcacgtata tacttttgct ctttcgatat cattcatatc aatatcatgc aaaagaaatc
    24961 aatttacgtc gcctacacgg gcgggaccat cgggatgcag cgttccgagc agggttatat
    25021 tccggtgtca ggtcatctac aacgccaact ggcgctgatg ccggaattcc atcgcccgga
    25081 gatgccagat ttcaccattc atgaatatac gccgttgatg gattcttctg atatgacgcc
    25141 ggaagactgg cagcatattg ctgaagatat taaagcgcac tatgacgact atgatggttt
    25201 tgtcattcta cacggtaccg acacgatggc gtataccgcc tctgcgctgt cgttcatgct
    25261 cgagaatctc ggtaaaccgg tcattgtgac agggtcacaa atcccgctgg ctgagttacg
    25321 ctctgacgga caaattaatc tgctgaatgc gttgtacgtt gcggcgaatt atccgatcaa
    25381 cgaagtaacg ctctttttca ataaccgatt gtatcgcggc aaccgcacta ccaaagccca
    25441 tgccgatggt tttgatgcgt ttgcctctcc aaaccttcct ccgttactgg aagctggtat
    25501 ccatattcgt cgtttgaata cgccacccgc cccgcacggt gaaggggaat taatcgttca
    25561 tccaatcacg ccacaaccaa ttggcgtagt gacgatttat ccagggattt ctgctgacgt
    25621 cgtgcgcaat tttctgcgcc aaccggtgaa agcattgatt ctgcgctcct atggcgtggg
    25681 taatgcgcca caaaacaaag ccttcctgca ggaattacaa gaagccagcg atcgcggtat
    25741 tgtggtggtc aacctgacac aatgtatgtc cggtaaagtg aatatgggtg gttatgccac
    25801 cggtaacgcc ctcgcccatg ccggcgttat tggcggtgca gatatgactg tagaagccac
    25861 gctaaccaaa ctgcattacc tgctgagcca ggaactggat actgaaacca ttcgcaaggc
    25921 catgagccaa aacctgcgcg gcgaactgac gccggatgat taaggagact gcaatgcccc
    25981 atcgcgccct gttactggtc gatttacaaa atgatttctg tgctggtggc gcgctcgccg
    26041 tgccggaagg tgacagtacg gtagatgtcg ctaaccgcct gattgactgg tgccagtcgc
    26101 gcggtgaagc ggttatcgcc agtcaggact ggcacccggc gaatcacggc agttttgcca
    26161 gtcagcacgg tgtagagcct tatacgccag gccaactcga cggtttgcca caaaccttct
    26221 ggccagatca ctgtgtgcag aacagtgaag gcgcacaatt acatccgtta ctgaaccaaa
    26281 aagcgatcgc agcggtgttc cataaaggcg aaaatccttt agttgacagt tacagtgcct
    26341 tttttgataa cggccgtcgg cagaaaacct ctctcgatga ctggttacgc gatcatgaaa
    26401 tcgatgaatt gatcgttatg ggcctggcta ctgactattg cgtgaagttt accgtgctgg
    26461 acgcgttaca gttaggttat aaggtaaacg tgattaccga tggttgtcgt ggcgtgaata
    26521 tccagcccca ggacagtgcg cacgcgttta tggagatgtc agcagctggg gcaacgctat
    26581 atacgctggc agactgggaa gagacgcagg ggtaatatta gttgaattca tctctggcct
    26641 acatttcctc aatgacattg taggccagat aatacttgtc agtgttgcat ttaacctact
    26701 tgcagctaaa acacgacgtc ctaacaagta tatttgccct cccttaataa caggcttaaa
    26761 tactagataa ctcattcact taaacatagc aataggcttt gctgagatac caaaatcttc
    26821 ttttaactgc tgtttgctct tcatgaccat ctgaccattt gtgtcaattg tcatatgctg
    26881 tgcttcggta ttatggcggg cttgccacag catcaccagt tgcaggcaat tctctttttg
    26941 ttcctcggtc aacgcaacgc catcaggcca tttccccagt tcaacggcgg tcgacaaacg
    27001 ctggtatacc tcaggcgtca tgctgttgat aatgtcatca agattcataa tttttccgct
    27061 cctgtggaat aagttgctga atcgtttttt caaccgttga tttcttcgcc gttttcgcca
    27121 tcagtgaagc gtaaagaagc agagttaaca caataacgtt cgcctgttgg ttgcggcccg
    27181 tcgggaaaga catgccccag atgggcatca cagttaccgc aacgaatttc tatgcgctgc
    27241 attccatgtg acaagtcttt gatataacga atggattctt cactcaccgg ttcgtagaaa
    27301 ctgggccagc cacagccgga atcatacttg gtttcggaat gaaacagcgg ggcatcgcag
    27361 atcaaacagt gatatacgcc gtcacgcttg ttatgcagta aacgacccgt aaatggcggt
    27421 tctgtcccat gattctgcgt cacgtaaaac tgcatctcgg acaaattttt tttcagttct
    27481 tctgccgaag gtttattagc catttgctca catctcactt taatcgtgct cacattacgt
    27541 gactgattct aacaaaacat taacaccaac tggcaaaatt ttgtcctaaa cttgatctcg
    27601 acgaaatggc tgcacctaaa tcgtgatgaa aatcacattt ttatcgtaat tgccctttaa
    27661 aattcggggc gccgacccca tgtggtctca agcccaaagg aagagtgagg cgagtcagtc
    27721 gcgtaatgct taggcacagg attgatttgt cgcaatgatt gacacgattc cgcttgacgc
    27781 tgcgtaaggt ttttgtaatt ttacaggcaa ccttttattc actaacaaat agctggtgga
    27841 atatatgact atcaaagtag gtatcaacgg ttttggccgt atcggtcgca ttgttttccg
    27901 tgctgctcag aaacgttctg acatcgagat cgttgcaatc aacgacctgt tagacgctga
    27961 ttacatggca tacatgctga aatatgactc cactcacggc cgtttcgacg gtaccgttga
    28021 agtgaaagac ggtcatctga tcgttaacgg taaaaaaatc cgtgttaccg ctgaacgtga
    28081 tccggctaac ctgaaatggg acgaagttgg tgttgacgtt gtcgctgaag caactggtct
    28141 gttcctgact gacgaaactg ctcgtaaaca catcaccgct ggtgcgaaga aagtggttat
    28201 gactggtccg tctaaagaca acactccgat gttcgttaaa ggcgctaact tcgacaaata
    28261 tgctggccag gacatcgttt ccaacgcttc ctgcaccacc aactgcctgg ctccgctggc
    28321 taaagttatc aacgataact tcggcatcat cgaaggtctg atgaccaccg ttcacgctac
    28381 taccgctact cagaaaaccg ttgatggccc gtctcacaaa gactggcgcg gcggccgcgg
    28441 cgcttcccag aacatcatcc cgtcctctac cggtgctgct aaagctgtag gtaaagtact
    28501 gccagaactg aatggcaaac tgactggtat ggcgttccgc gttccgaccc cgaacgtatc
    28561 cgtagttgac ctgaccgttc gtctggaaaa agctgcaact tacgagcaga tcaaagctgc
    28621 cgttaaagct gctgctgaag gcgaaatgaa aggcgttctg ggctacaccg aagatgacgt
    28681 agtatctacc gatttcaacg gcgaagtttg cacttccgtg ttcgatgcta aagctggtat
    28741 cgctctgaac gacaacttcg tgaaactggt atcttggtac gacaacgaaa ccggttactc
    28801 caacaaagtt ctggacctga tcgctcacat ctccaaataa gttgagatga cactgtgatc
    28861 taaaaagagc gacttcggtc gctctttttt ttacctgata aaatgaagtt aaaggactgc
    28921 gtcatgatta agaaaatttt tgcccttccg gtcatcgaac aaatctcccc tgtcctctcc
    28981 cgtcgtaaac tggatgaact ggacctcatt gtggtcgatc atccccaggt aaaagcctct
    29041 tttgcattac agggcgcaca ccttctctcg tggaaacctg cgggtgaaga agaagttctg
    29101 tggttgagca acaacacacc gttcaaaaat ggcgtcgcta ttcgcggtgg cgtaccggtt
    29161 tgctggccgt ggtttggtcc ggcggcacaa caaggtctgc ctgcgcacgg ttttgcccgc
    29221 aacctgccgt ggacgctgaa atcacatcgt gaagatgctg atggcgtagc gctgactttt
    29281 gaattgacgc aaagcgaaga gacgaaaaaa ttctggccgc acgactttac gctgttagcg
    29341 catttccgcg tgggtaaaac ctgtgaaatc gatcttgaat cacatggcga atttgaaacc
    29401 acctctgccc tacataccta ctttaacgtg ggtgatatcg ctaaggtaag cgtcagtggg
    29461 ctgggcgatc gcttcattga taaagtaaat gacgcgaaag aagatgtact gaccgacggt
    29521 attcagacct tccctgaccg taccgatcgc gtgtatctga atccgcaaga ttgcagcgtg
    29581 attaatgatg aagcgctgaa tcgtattatc gccgtaggcc accagcatca tctgaacgtt
    29641 gtcggctgga acccgggacc ggcgctttca gttagcatgg gcgacatgcc ggatgatggc
    29701 tacaaaacat ttgtttgtgt agaaacggct tacgcttcag aaacgcaaaa agtgaccaaa
    29761 gagaaacctg cacatctggc gcaatccatt cgcgttgcga aacgttaatt tgcgttaatg
    29821 ttgtgtgccg ggtgcgatgc atccggcacg tatcaacatc acaccatatc cagcgcagtt
    29881 tttccttttg gtgccggata cgctttatcc agcatggtta attccgctga cgaaagttcg
    29941 acctcaagca cagccgcatt ctgttggaca tgggcaatcg tggccgcttt tggaatcgcc
    30001 atcacaccct gatggctgat tacccacgcc aacaatactt gtgccgcgct gatattgtga
    30061 gcatgtgcaa tttcgttgac taccgcgttt tttaacagtc cattgcgcaa ccgcccggcc
    30121 tgggctaatg gactgtaagc catcaccggc atctgctgtt gctggcacca ggggagtaga
    30181 tcgtactcaa tccctcgtga accgagatgg taaagcacct gattagtggc acactgattt
    30241 ccccccggca gctgccagag ttcctgcata tcagcatgat caaggttaga aacgccccag
    30301 cggcggattt ttccctgggc gatcaatttt tccatcgctg ccacagtctc ttcaaaagcg
    30361 aaactgccag accagtgtaa taagtagaga tcgagataat cagtattgag acggcgtaaa
    30421 ctggcttcgc atgcatttat cgctttttgc ccgccagcat tccacggata gactttagag
    30481 acgagaaaga cgttatcgcg cagaccggtt aatgcttccc caaccacctt ttcggcaccg
    30541 ccatcggcat acatttcggc ggtatcaatg agggttaaac cgagctcaat gcccgcgcgt
    30601 agtgcagcaa cttctgtttt gcgctgactg gcatcttcgc ccatatacca tgttccctgc
    30661 cctatggctg gcagtgagac atcgccacta aattgaatca ttttttgttg cattgtttcc
    30721 tcccaggtat tgcaccaccg taatgcaaaa cagggcgtga cgccctgttt ttatgcacaa
    30781 aatgccctgg aaagatgcat tatcagaatt tgtaggtgat cccggtagaa atcaggccag
    30841 tccaggattt atccaccatc gggctgtcag taacttcatc agacagacgg gtatagcgcg
    30901 cggtaccgta aacactccag tcgccgagga agttgtagct ggcgctcagc tccaggtaag
    30961 ggctccagct gtcgttcggg ttatagccac gcagaccgct gcgagcggac tctttgcgcg
    31021 atacgccata atagtattcg ttctggtttt cgctgttcca ctgcacaccg atacccgggg
    31081 tcacggtcag gccgccgttg gtgtaacgat acaaccaggc catatcccag acgatgccgt
    31141 tgctgttatc cagggtatcg ccagccaggg tggtacgcag gtaaccgtac tgggtaaagt
    31201 gagcataaga cagaccagcc atcatggtgc tcttacggtc atccaggtga cgcatttggt
    31261 gatcgccact gtctttcgct ttgaagtaaa gcggcgacca ataagcggta attgaaagtt
    31321 tgtccgttgc gtcattccac aggtagtaac caccacctaa gccacggaac cagaagttat
    31381 cgccttcata gttgattacc ggtactgggt aaacatcggt atcgtaatct ttatatgggt
    31441 gctcaacgac acctacgcct gcgcccaggg aaaatttacc ttcagcgtgc gctacgcctg
    31501 cagacgttgc gataagcact ccaagtgcca gaagtttgag tttggtcaca attaatcatt
    31561 ccttaaacaa atgtttagcg ggcgacaaag tttacccgtc aatacatcga aacccaacct
    31621 ttttacgttt tcatttttta aagtaactgt ttaattttcc tgacgcggat gacaccgcgc
    31681 ttacagccaa atgaatttag cgttactggc taccctggtc tttacattaa ttatgcaaaa
    31741 tttatggatg agttgttgat atgccattga aattaagaaa gccgtgcagg caagttttcc
    31801 atttgccatc tacgcttaat tttgaaggtg tatcaccggg cacgttgttc tcatcgtcga
    31861 taaaatggca tgagagttgc tgtgttttag caagagacgt cgttcagttt acctcttccg
    31921 ggagcctcta ctattcatat gaacggctct taacctgtgc taaaaaacga aaggacggca
    31981 taccatgaat atattcgatc actatcgcca gcgatatgaa gctgccaagg acgaagagtt
    32041 cacactgcag gagtttctta ccacttgtcg gcaagatcgc agtgcttatg ccaacgcggc
    32101 tgagcggcta ttgatggcta tcggtgagcc tgtcatggtc gatacagccc aggaacccag
    32161 actttctcga ctcttttcta accgggtcat tgcacgttat ccggcatttg aagagtttta
    32221 cggcatggaa gacgcgattg aacagattgt ctcttatctg aaacacgcgg ctcaggggct
    32281 ggaagagaag aaacaaatcc tgtatctgct ggggcctgtg ggtgggggta aatcatcgct
    32341 tgctgagcga ctgaaatcat taatgcagct cgtaccgatt tatgtattga gcgccaacgg
    32401 tgagcgtagc ccggtcaacg atcatccgtt ctgtcttttc aatccgcagg aagatgctca
    32461 gattctggaa aaagagtatg gcattcctcg ccgttatctc ggcaccatca tgtcgccatg
    32521 ggcggcaaaa cgcctgcatg aatttggtgg cgatatcact aagttccggg tagtgaaggt
    32581 ctggccgtca attctgcaac aaattgctat cgccaaaacg gaacccggtg atgagaacaa
    32641 ccaggacatc tccgcgctgg ttgggaaagt cgatattcgt aaactcgaac actacgcgca
    32701 gaatgacccg gacgcctacg gctattccgg tgcgctgtgc cgcgccaacc aagggatcat
    32761 ggaatttgtc gagatgttta aagcaccgat aaaagtgctg catccgctgt taaccgccac
    32821 tcaggaaggt aactacaacg ggacggaagg tatctccgcc ctgccgttca acgggattat
    32881 tctcgcccac tcgaacgaat ccgaatgggt cactttccgt aataacaaaa acaacgaagc
    32941 cttcctcgac cgtgtttaca tcgtgaaggt gccgtattgc ttacgcattt ccgaagagat
    33001 caaaatctac gagaaattgc ttaatcacag tgaattgact cacgccccct gcgcccctgg
    33061 cacgctggaa acgctgtcac gtttttccat tctttcgcgc cttaaagagc cagaaaactc
    33121 cagcatttat tcaaagatgc gggtttatga tggcgaaagt ctgaaagaca ccgatcccaa
    33181 agccaagtcg tatcaggaat atcgtgacta cgccggtgtc gatgaaggga tgaacggtct
    33241 gtcgacgcgt tttgcgttta agatcctctc ccgcgtgttc aacttcgatc atgtagaagt
    33301 ggcggcaaac ccggtccatc tgttctacgt cctggaacag cagatcgagc gcgagcagtt
    33361 cccacaagag caggcagaac gctatctgga gttcctgaaa ggttatctga tcccgaaata
    33421 tgccgagttt atcggcaaag agatccagac ggcctacctt gaatcctatt ccgaatatgg
    33481 gcaaaacatt ttcgaccgtt atgttaccta cgcggatttc tggattcagg atcaggagta
    33541 tcgcgatccg gataccgggc agctgtttga ccgcgagtct cttaacgccg agctggagaa
    33601 aatcgagaaa ccggcgggga tcagtaatcc aaaagatttc cgcaacgaga ttgttaactt
    33661 cgtactgcgc gccagagcga ataacagcgg acgcaatccg aactggacca gctatgaaaa
    33721 actgcgcacg gttatcgaga agaaaatgtt ctccaatacc gaggagctgt tgccggttat
    33781 ctcgtttaac gccaaaacgt caaccgacga gcagaagaaa cacgacgact ttgtcgaccg
    33841 tatgatggaa aaaggctaca cccgtaaaca ggtgcgttta ctgtgcgaat ggtatttgcg
    33901 cgtacgtaaa tcgtcttaac aaccctggcc cggtacacat gttaccgggc ctacaacgac
    33961 agcgaaccgt gggcctgaga agcggcaaca caggcgtagc atacagttgg caaatgtagt
    34021 acggggggca tatgacctgg tttattgacc ggcgtctgaa cggcaaaaac aaaagcatgg
    34081 tgaatcgcca gcgtttttta cgccgttata aagcgcaaat taaacagtcg atctccgagg
    34141 ccattaataa gcgttcggtg actgacgtcg acagcggcga atccgtatcc attcccacgg
    34201 aagatattag cgaaccgatg tttcatcagg ggcgtggcgg tctgcgccac cgcgtgcatc
    34261 cgggcaatga ccatttcgtc cagaacgacc gaattgaacg tccccagggt ggcggcggag
    34321 gttccggtag tggtcagggc caggccagcc aggatggtga aggtcaggat gaatttgtct
    34381 ttcagatttc gaaagatgag tatcttgatt tgctctttga agatttggcc ttaccgaatc
    34441 tgaaacaaaa ccaacaacgc cagctgaccg aatataaaac gcatcgggcg ggttataccg
    34501 ctaacggcgt tccggcaaat atcagcgttg tgcgctcctt gcagaactca ctggcgcgac
    34561 gcacagccat gacggcaggc aagcggcggg aacttcatgc actggaagag aatttggcca
    34621 tcatcagcaa cagtgaacct gcgcagctgc tggaagagga acgtctgcgc aaagaaattg
    34681 cagaattacg tgccaaaatt gaacgcgtcc cttttattga caccttcgat ttacgttaca
    34741 agaactacga gaagcggccc gatccctcca gccaggcagt gatgttttgc ctgatggacg
    34801 tttccggttc aatggatcaa tccactaaag atatggctaa gcgtttttat attctgctgt
    34861 atctgttcct cagcagaacg tataagaacg tggaagtcgt atacatccgc catcataccc
    34921 aggcgaaaga agtcgatgaa catgagtttt tctactcgca ggaaacaggc ggcaccattg
    34981 tttccagcgc cctgaaactg atggatgagg tagtgaaaga gcgttataac ccggcacagt
    35041 ggaatattta cgctgcacaa gcatcggacg gcgataactg ggccgatgac tctccgcttt
    35101 gccatgaaat cctggcgaaa aaattattac ctgttgttcg ttattacagc tatatcgaaa
    35161 ttacccgtcg tgcacatcag acattgtggc gagaatatga gcatctgcaa tctactttcg
    35221 acaactttgc gatgcagcac atccgcgacc aggatgatat ttatccggtg ttccgtgaac
    35281 tgtttcataa acaaaatgca acagctaaag actaaaacta tcagccaggt cattatcgcc
    35341 tggctgattt ttagcttact gtaaattatc tcatttatta catacattag cttacaatcg
    35401 ctttaaatat gacagcgtaa cctttacata atttagttcc agaaaacaat cattcggaaa
    35461 aatgattcag tcaacacgta tttccatggg gttattcttt aaatattgtt tatcgttaa
//
LOCUS       DAZFRJ010000027        35036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000027 DAZFRJ010000000
VERSION     DAZFRJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.027"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            206..1300
                     /gene="gcvT"
                     /locus_tag="ACHKUN_001224"
     CDS             206..1300
                     /gene="gcvT"
                     /locus_tag="ACHKUN_001224"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO6659522.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1324..1713
                     /gene="gcvH"
                     /locus_tag="ACHKUN_001225"
     CDS             1324..1713
                     /gene="gcvH"
                     /locus_tag="ACHKUN_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO6659523.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1832..4705
                     /gene="gcvP"
                     /locus_tag="ACHKUN_001226"
     CDS             1832..4705
                     /gene="gcvP"
                     /locus_tag="ACHKUN_001226"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO6659524.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            4966..5709
                     /gene="ygfF"
                     /locus_tag="ACHKUN_001227"
     CDS             4966..5709
                     /gene="ygfF"
                     /locus_tag="ACHKUN_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6659525.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYTASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5766..7199)
                     /gene="bglA"
                     /locus_tag="ACHKUN_001228"
     CDS             complement(5766..7199)
                     /gene="bglA"
                     /locus_tag="ACHKUN_001228"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO6659526.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7244..7555
                     /gene="yqfB"
                     /locus_tag="ACHKUN_001229"
     CDS             7244..7555
                     /gene="yqfB"
                     /locus_tag="ACHKUN_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO6659527.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7719..8378
                     /gene="yqfA"
                     /locus_tag="ACHKUN_001230"
     CDS             7719..8378
                     /gene="yqfA"
                     /locus_tag="ACHKUN_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO6659528.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8574..9554)
                     /gene="ygfZ"
                     /locus_tag="ACHKUN_001231"
     CDS             complement(8574..9554)
                     /gene="ygfZ"
                     /locus_tag="ACHKUN_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO6659529.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9797..10063
                     /gene="sdhE"
                     /locus_tag="ACHKUN_001232"
     CDS             9797..10063
                     /gene="sdhE"
                     /locus_tag="ACHKUN_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO6659530.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10044..10451
                     /gene="ygfX"
                     /locus_tag="ACHKUN_001233"
     CDS             10044..10451
                     /gene="ygfX"
                     /locus_tag="ACHKUN_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO6659531.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10491..11012)
                     /gene="fldB"
                     /locus_tag="ACHKUN_001234"
     CDS             complement(10491..11012)
                     /gene="fldB"
                     /locus_tag="ACHKUN_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO6659532.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11124..12020
                     /gene="xerD"
                     /locus_tag="ACHKUN_001235"
     CDS             11124..12020
                     /gene="xerD"
                     /locus_tag="ACHKUN_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO6659533.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLRAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12045..12755
                     /gene="dsbC"
                     /locus_tag="ACHKUN_001236"
     CDS             12045..12755
                     /gene="dsbC"
                     /locus_tag="ACHKUN_001236"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO6659534.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12761..14494
                     /gene="recJ"
                     /locus_tag="ACHKUN_001237"
     CDS             12761..14494
                     /gene="recJ"
                     /locus_tag="ACHKUN_001237"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO6659535.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14585..15683
                     /gene="prfB"
                     /locus_tag="ACHKUN_001238"
     CDS             join(14585..14659,14661..15683)
                     /gene="prfB"
                     /locus_tag="ACHKUN_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO6659536.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15693..17210
                     /gene="lysS"
                     /locus_tag="ACHKUN_001239"
     CDS             15693..17210
                     /gene="lysS"
                     /locus_tag="ACHKUN_001239"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6659537.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17253..17801)
                     /gene="idi"
                     /locus_tag="ACHKUN_001240"
     CDS             complement(17253..17801)
                     /gene="idi"
                     /locus_tag="ACHKUN_001240"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO6659538.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17856..17927
                     /locus_tag="ACHKUN_001241"
     CDS             17856..17927
                     /locus_tag="ACHKUN_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659539.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17924..18049)
                     /gene="yqfG"
                     /locus_tag="ACHKUN_001242"
     CDS             complement(17924..18049)
                     /gene="yqfG"
                     /locus_tag="ACHKUN_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO6659540.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18051..19499)
                     /gene="uacT"
                     /locus_tag="ACHKUN_001243"
     CDS             complement(18051..19499)
                     /gene="uacT"
                     /locus_tag="ACHKUN_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO6659541.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19935..21854
                     /gene="ygfT"
                     /locus_tag="ACHKUN_001244"
     CDS             19935..21854
                     /gene="ygfT"
                     /locus_tag="ACHKUN_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO6659542.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21854..22342
                     /gene="ygfS"
                     /locus_tag="ACHKUN_001245"
     CDS             21854..22342
                     /gene="ygfS"
                     /locus_tag="ACHKUN_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6659543.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22378..23745)
                     /gene="ghxQ"
                     /locus_tag="ACHKUN_001246"
                     /gene_synonym="ygfQ"
     CDS             complement(22378..23745)
                     /gene="ghxQ"
                     /locus_tag="ACHKUN_001246"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO6659544.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23781..25097)
                     /gene="guaD"
                     /locus_tag="ACHKUN_001247"
     CDS             complement(23781..25097)
                     /gene="guaD"
                     /locus_tag="ACHKUN_001247"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO6659545.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25115..26515)
                     /gene="xanQ"
                     /locus_tag="ACHKUN_001248"
     CDS             complement(25115..26515)
                     /gene="xanQ"
                     /locus_tag="ACHKUN_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO6659546.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26680..29550)
                     /gene="xdhD"
                     /locus_tag="ACHKUN_001249"
     CDS             complement(26680..29550)
                     /gene="xdhD"
                     /locus_tag="ACHKUN_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO6659547.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29547..30326)
                     /gene="ygfM"
                     /locus_tag="ACHKUN_001250"
     CDS             complement(29547..30326)
                     /gene="ygfM"
                     /locus_tag="ACHKUN_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO6659548.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30377..31705)
                     /gene="ssnA"
                     /locus_tag="ACHKUN_001251"
     CDS             complement(30377..31705)
                     /gene="ssnA"
                     /locus_tag="ACHKUN_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO6659549.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31708..34806)
                     /gene="ygfK"
                     /locus_tag="ACHKUN_001252"
     CDS             complement(31708..34806)
                     /gene="ygfK"
                     /locus_tag="ACHKUN_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO6659550.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         8589 a         8896 c         9286 g         8265 t
ORIGIN      
        1 ttattctgtt gcgattttgg cgttttttaa ccataagcta atgtgatgat caattttacc
       61 ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg
      121 accgggagtg gctccgatgc tgggtttcgt ggtgataatt tcaccatgaa aaagttgtca
      181 gccccgctta ttcaatgagg acaagatggc acaacagact cctttgtacg aacaacacac
      241 gctttgcggc gctcgcatgg tggatttcca cggctggatg atgccgctgc attacggttc
      301 gcaaatcgac gaacatcatg cggtacgtac cgatgccgga atgtttgatg tgtcacatat
      361 gaccatcgtc gatcttcgcg gcagccgcac ccgggagttt ctgcgttatc tgctggcgaa
      421 cgatgtggcg aagctcacca aaagcggcaa agccctttac tcggggatgt tgaatgcctc
      481 tggcggtgtg atagatgacc tcatcgtcta ctactttact gaagatttct tccgcctcgt
      541 tgttaactcc gccacccgcg aaaaagacct ctcctggatt acccaacacg ctgaaccttt
      601 cggcatcgaa attaccgttc gtgatgacct ttccatgatc gccgtacaag ggccgaatgc
      661 gcaggcaaaa gctgccacac tgtttaatga tgctcagcgt caggcggtgg aagggatgaa
      721 accgttcttt ggcgtgcagg cgggcgatct gtttattgcc accactggtt ataccggtga
      781 agcgggctat gaaattgcgc tgcccaatga aaaagcggcc gatttctggc gtgcgctggt
      841 ggaagcgggc gttaagccat gcggtctggg cgcgcgtgac acgctgcgtc tggaagcggg
      901 catgaatctt tatggtcagg agatggacga aactatttct cctttagccg ccaacatggg
      961 ctggactatc gcctgggaac cggcagatcg tgacttcatc ggtcgtgaag ccctggaagt
     1021 gcagcgtgag catggcacag aaaaactggt tggtctggtg atgaccgaaa aaggcgtgct
     1081 gcgtaatgaa ctgccggtac gttttaccga tgcgcagggc aaccagcatg aaggcattat
     1141 caccagcggg actttctccc cgacgctggg ttacagcatt gcgctggcac gcgtgccgga
     1201 aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgccgg ttaaagtgac
     1261 gaaacctgtt tttgtgcgta acggtaaagc cgtcgcgtga tttacttttt tggagattga
     1321 ttgatgagca acgtaccagc agaactgaaa tacagcaaag aacacgaatg gctgcgtaaa
     1381 gaagccgacg gcacttacac cgttggtata accgaacatg cccaggagct gttaggcgat
     1441 atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta gcgcgggcga tgactgcgcg
     1501 gttgccgaat cagtaaaagc ggcgtcagac atttatgcgc cagtaagcgg tgaaatcgtg
     1561 gcggtaaacg acgcactgag cgattccccg gaactggtga acagcgaacc gtatgcaggt
     1621 ggctggatct ttaaaatcaa agccagcgat gaaagcgaac tggaatcact gctggatgcg
     1681 accgcatacg aagcattgtt agaagacgag taacggcttt attcctcttc tgcgggagag
     1741 gatcagggtg aggaaaattt atgcctcacc ctcactctct tcgtaaggag agaggttcac
     1801 aattcactgc acgtttcagg aaccatcgct catgacacag acgttaagcc agcttgaaaa
     1861 cagcggcgct tttattgaac gccatatcgg accggacgcc gcgcaacagc aagaaatgct
     1921 gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc cagattgtgc cgaaagatat
     1981 tcagcttgcg accccgccgc aggttggcgc accggcgacc gaatacgccg cactggcaga
     2041 actcaaggct attgccagtc gcaataaacg cttcacgtct tacatcggca tgggttacac
     2101 cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg gaaaatccgg gctggtatac
     2161 cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc cttgaagcac tgctcaactt
     2221 ccagcaggta acgctggatt tgactggact ggatatggcc tctgcttctc ttctggacga
     2281 ggccaccgct gccgccgaag caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc
     2341 caaccgcttc ttcgtggctt ccgatgtgca tccgcaaacg ctggatgtgg tccgtactcg
     2401 tgccgaaacc tttggttttg aagtgattgt cgatgacgcg caaaaagtgc tcgaccatca
     2461 ggacgtcttc ggcgtgctgt tacagcaggt tggcacaacc ggtgaaatcc acgactacac
     2521 cgcgcttatt agcgaactga aatcacgcaa aattgtggtc agtgtcgccg ccgatattat
     2581 ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg gatattgttt ttggttcggc
     2641 gcaacgcttc ggcgtgccga tgggctacgg tggcccacac gcggccttct ttgcggcgaa
     2701 agatgaatac aaacgctcaa tgccgggccg tattatcggt gtatcgaaag atgcagctgg
     2761 caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa catattcgcc gtgagaaagc
     2821 gaactccaac atttgtactt cccaggtgct gctggcaaac atcgccagcc tgtatgccgt
     2881 ttatcacggc ccgattggcc tgaaacgtat cgctaaccgc attcaccgtc tgaccgatat
     2941 cctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc catgcgcact atttcgacac
     3001 cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg cgtgccgaag cggctgaaat
     3061 caacctgcgt agcgatattc tgaacgcggt tgggatcacc cttgatgaaa caaccacgcg
     3121 tgaaaacgta atgcagcttt tcagcgtact actgggcgat aaccacggcc tggacatcga
     3181 cacgctggac aaagacgtgg ctcacgacag ccgctctatc cagcctgcga tgctgcgcga
     3241 cgacgaaatc ctcacccatc cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg
     3301 ctatatgcac tcgctggagc gtaaagatct ggcgctgaat caggcgatga tcccgctggg
     3361 ttcctgcacc atgaaactga acgccgccgc cgagatgatc ccaatcacct ggccggaatt
     3421 tgccgaactg cacccgtttt gtccgccgga acaggctgaa ggttatcagc agatgattgc
     3481 gcagctggct gactggctgg tgaaactgac cggttacgac gccgtttgta tgcagccgaa
     3541 ctccggcgca cagggcgaat acgcgggcct gctggcgatt cgtcattatc atgaaagccg
     3601 caacgaaggg catcgcgata tctgcctgat cccggcttct gcgcacggaa ctaaccccgc
     3661 ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg tgtgataaaa acggcaacat
     3721 cgatctgact gatctgcgcg cgaaagcgga acaggcgggc gataacctct cctgtatcat
     3781 ggtgacttat ccttctaccc acggcgtgta tgaagaaacg atccgtgaag tgtgtgaagt
     3841 cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg aacatgaacg cccaggttgg
     3901 catcacctcg ccgggcttta ttggcgcgga cgtttcgcac ctcaacctgc ataaaacttt
     3961 ctgcattccg cacggcggtg gtggtccggg tatgggaccg atcggtgtga aagcgcatct
     4021 ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg
     4081 cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg ccaatcagct ggatgtacat
     4141 ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag gtggcaatcc tcaacgccaa
     4201 ctatattgcc agccgcctgc aggatgcctt cccggtgctg tataccggtc gcgacggtcg
     4261 cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa gaagaaaccg gcatcagcga
     4321 gctggatatt gccaagcgcc tgatcgacta cggtttccac gcgccgacca tgtcgttccc
     4381 ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg
     4441 ctttatcgac gcgatgctgg ctatccgcgc agaaatcgac caggttaaag ccggtgtctg
     4501 gccgctggaa gataacccgc tggtgaacgc gccgcacatt cagaacgaac tggtcgccga
     4561 gtgggcgcat ccgtacagcc gtgaggttgc agtattcccg gcaggtgtgg cagacaaata
     4621 ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac cgtaacctgt tctgctcctg
     4681 cgtaccgatt agcgaatacc agtaattcac tgattcgact attttctaaa ggcgcttcgg
     4741 cgccttttta gtcagatgac aaagtacaaa agtgctcaga cagtcccctc gcccctttgg
     4801 ggagagggtg aggggaacag gccggcactg gcgcgaatat ttaccctcac cccggccctc
     4861 tccctgaaag ggcgaggggg aaaagcgtgc caacattgaa gattgagcca gtttgttagc
     4921 aatctcaaag atacgtcaac gaatgaattt ttctcggaaa aacaaatggc tatagcactt
     4981 gtgaccggtg gcagtcgcgg catcgggcgg gcaactgcat tactgttggc gcaagaaggg
     5041 tatacggtgg cggttaatta tcagcaaaac ctccacgcgg cgcaggaagt gatgaactta
     5101 ataacgcaag ccggtggcaa agcattcgtg ctccaggcgg atatcagcga cgaaaaccag
     5161 gtcgttgcga tgtttacagc aatcgatcag cacgatgaac cgctagcagc gctggtcaat
     5221 aacgccggga tcttgtttac ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac
     5281 cgagtacttt ccaccaacgt gacgggatat tttctctgct gccgcgaggc ggtaaaacgc
     5341 atggcgctta aaaatggtgg cagtggcggc gctatcgtca atgtctcttc ggtggcctca
     5401 cggttgggtt cgccagggga atatgttgat tacacggcat cgaaaggggc gattgatacg
     5461 ttaaccaccg gactatcgct ggaagtcgcc gcgcagggga tccgcgttaa ctgcgtgcgg
     5521 ccagggttta tttataccga aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc
     5581 gttaagtcga acatccccat gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt
     5641 gtctggctac taagtgataa agcctcttac gtcacgggaa gttttatcga tttggcgggc
     5701 gggaaataaa acagggaagt tgtctgaccg gatgcaacaa gtattgcatc cggtacttca
     5761 tcgacttaaa gcttctcgcc gttgctggca atcacctctt ggtaccagtt aaagctcttc
     5821 ttacgtgaac gcgacatatc gccagcaccg tcgtcatgtt tattcacata gataaagccg
     5881 tagcgtttgc tgtactgccc ggtggtgaac gatacgcagt cgatgcagcc ccacggtgtg
     5941 tagcccatca gatccacgcc atcgtaagtc accgcttttt tcatctcttc gatatgggcg
     6001 cgcaggtagt caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa
     6061 gcgccaaaac cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa
     6121 agggcatagc gcagacctac tggatcaatc tgccagcccc agtcagacgc tttaacatac
     6181 gggttcggta cgctgccttc gaaaccagag atcgcatcgc cggtgccgcc ttcggccttc
     6241 actgcgttgg tcatgtaata gctgaaacca agataatcgc aggtgccttc acgcagtaca
     6301 tcgagatcgc cgtcttccat tttgatgtta aatccgcgac gctcccactc gttcaacaca
     6361 taggacgggt aatagccgcg tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac
     6421 tcctgagcga acatcacatc gtccgggtta cagcagtagg gatagagcgg caccatcgcc
     6481 agcatacagc cgactttcat ctccgggtga atgcgacgcg cagctttcac cgccagggcg
     6541 ctggcgacaa actggtgatg cagcacctga tacatcgtct cttccgggtt ctcatgctcg
     6601 gtataaacca cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg
     6661 ttaatttcgt tgaaggtcat ccagtatttg actttatgct tatagcgttc aaacaccact
     6721 tccgcgaaac ggacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc
     6781 accagatgca gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc
     6841 agcagttcat cgaacatatc atcgtagaac ttcagccctt cttcgtttgg ctgagcttca
     6901 tcaccttttg gaaaaatacg cgtccaggcg atggaagtcc gaaaacattt gaagcccatt
     6961 tcggcaaata gcttgatgtc ttccttgtag tgaccataaa aatcaacggc ttcatggttt
     7021 ggatagtatt tgcctggcaa gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca
     7081 ccggtcagaa cgtcacaaat gctcggccct tttccgcctt tgttccagcc gccttcgacc
     7141 tgatgagcgg caactgcgcc gccccataag aaatctttcg gtaaggtgag ttttttcact
     7201 atcataggct ccagatgagt caattcttac aggaaatagt accatgcagc caaacgacat
     7261 cacttttttt caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg
     7321 cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga
     7381 tgacggttat ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacgct
     7441 gactgaaaaa catgcagagc aggaaaatat gaccctgact gaactgataa aggtcattgc
     7501 cgacatctat cccggtcaga cacagtttta tgtgattgaa tttaaatgtc tttaaattga
     7561 actcgcgcct gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc
     7621 aaatcagaat ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag
     7681 aggtatacat gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta
     7741 agcagggata ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggttgg
     7801 tgtttggtat cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg
     7861 ccactgcgat aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt
     7921 cgacgctcta tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc
     7981 attgcgctat ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg
     8041 attctccgtt agcgcgcggg ttgatgattg ttatctggag cctggcattg ctgggtattc
     8101 tgtttaaact gaccatcgcg caccgattca aaattttatc tctggtgacc tatctggcga
     8161 tgggctggct gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg
     8221 ttaccttact ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca
     8281 aacgcattcc atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc
     8341 actttctggc gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg
     8401 ataagacgcg ccagcgtcgc atcaggcatc ggagcacaac tgccggattc ggcgtgaacg
     8461 ccttatccga tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga
     8521 taagacgcgt cagcgtcgca tcaggcgata aatgcctgat gcgataaaga gatttactct
     8581 tcgagcgaat acggcagcgg ctcgatatgc aatgtattcg catcgtcgcg tacgcggaag
     8641 atgctatccg gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc
     8701 agttttaccg cagccagcac cgtaccggta cgacgccagt tctcgcccat tttcagctct
     8761 aagtcttcac cagcttccgg cagtcggctg gcgctacctg ccagcaacca gagcgcacgt
     8821 ttattggcac cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc
     8881 ttaaagctga taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg
     8941 ttggcggcat caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta
     9001 ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg
     9061 gttacgatca ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct
     9121 ttgactacct gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc
     9181 gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt ctggcgcgat ggtcacttta
     9241 gagaataccg catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca
     9301 atccatgcaa agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg
     9361 gcgtcgcaat gggcggcgag caggtgctga ttttctgtca tctggctgac atctgctgtc
     9421 acctgaccct gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca
     9481 tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg taggctgacg gggaggaaaa
     9541 ggtgtaaaag ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa
     9601 aagagctatg aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac
     9661 ttccaaaaaa atgaagccat tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt
     9721 actgatattc ccgtaaaaga aggcgaaaaa cacgttacaa tagcggggta ctggattttc
     9781 gtgagacagg aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg
     9841 tggtatgcgc gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt
     9901 aagcgatgac gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt
     9961 taactggctg atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga tggtccgact
    10021 catccagaca cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc
    10081 gcgcacagtg gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc
    10141 cctggccact cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt
    10201 gcgttcgcag ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg
    10261 ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga
    10321 gcggcatgat gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag
    10381 ccgacagcat ggacgaagct gaatggcggg atttacggcg gattttgttg caacaagaga
    10441 cgcaaagata aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa
    10501 tgctctgcca tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg
    10561 tcatactggt tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc
    10621 ggtttcgggc tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct
    10681 ttggtcgaga gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc
    10741 agttgatcgc caagcccata cagcgcaaca attttacctt caaggttcag gtcgtcgagc
    10801 tgatcccaga cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc
    10861 agaatgagca catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag
    10921 gtcaccagtt ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag
    10981 gtgctggaac cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc
    11041 ttttgcagct agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa
    11101 atgagtgacc ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg
    11161 atgctctgtg gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc
    11221 tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg
    11281 acgatttgca ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag
    11341 cgcgtttgct gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg
    11401 aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt
    11461 taagcgaagc gcaggtcgaa cgtttattac gggcaccatt aattgatcag ccactggagc
    11521 tacgcgataa agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg
    11581 tcggactgac aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag
    11641 gcaacaaaga gcgcctggta ccgttaggtg aagaggcggt ttactggctg gaaacctatc
    11701 tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc
    11761 agcgtgcgca gcagatgacg cgacagacct tctggcaccg cattaaacat tatgctgtgc
    11821 tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc
    11881 atttattaaa tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc
    11941 tttccaccac gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac
    12001 agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta
    12061 tgttgtttac tttgttagcg gcgttttcag gttttgctca ggctgatgac gcggcaattc
    12121 aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg
    12181 gcatgaagac agttctgact aacagcggcg tgttgtacat caccgatgat ggtaaacata
    12241 tcattcaggg gccaatgtat gacgttagcg gcacggctcc ggtcaatgtc accaataaga
    12301 tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg
    12361 aaaaacacgt catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg
    12421 agcaaatggc agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc
    12481 aggggctgga cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa
    12541 acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg
    12601 atattgccga ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg
    12661 tgctgagcaa tggcacactt gttccgggtt accagccgcc gaaagagatg aaagaattcc
    12721 tcgacgaaca ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact
    12781 tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg
    12841 ccgtttatac gccagccggg gcgtgcgcag tgcgcaagaa ctggaacgca gtgttaaagg
    12901 tatgttgccc tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc
    12961 ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag
    13021 cacggctcta agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt
    13081 accaaaccgt ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc
    13141 ccgtggcgcg cagttaattg tcacggtgga taacggtatt tcctcccatg cgggggttgt
    13201 acacgctcgc tcgttgggca ttccggttat tgttaccgat caccatttgc caggcgaaac
    13261 attacccgcg gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa
    13321 atcactggca ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg
    13381 cgatcagggc tggtttgatg agcgtggcat cgcaattcct aacctggcag aactgctgga
    13441 tctggtcgcg ctgggaacag tggcggacgt cgtgccgctg gacgctaata atcgcattct
    13501 gacctggcag gggatgagtc gcatccgtgc cggaaagtgc cgtccgggga ttaaagcgct
    13561 gctggaagtg gcaaatcgcg atgcgcaaaa actcgccgcc agcgatttag gttttgcgct
    13621 ggggccacgt ctcaatgctg ccgggcgact ggacgatatg tccgtcggtg tggcgctgtt
    13681 gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca
    13741 gacgcgaaaa gagatcgaac aaggaatgca agttgaagcc ctgaccctgt gcgagaaact
    13801 ggagcgaagt cgcgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca
    13861 gggcgttgtc ggtattctgg cttcgcgcat caaagagcgt tttcaccgtc cggttatcgc
    13921 ctttgcgcca gcaggtgatg gtacgctgaa aggttcaggt cgctccattc aggggctgca
    13981 tatgcgtgat gcactggagc gattagacac actctaccct ggcatgatac tgaagtttgg
    14041 cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca
    14101 acggtttggc gaactggtta cagagtggct ggacccttcg ctattgcaag gcgaagtggt
    14161 gtcagacggt ccgttaagcc cggccgaaat gaccatggaa gtggcgcagt tgctgcgcga
    14221 tgctggcccg tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct
    14281 gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg
    14341 tccactgctg gatggtattg cttttaatgt cgataccgcc ctctggccgg ataacggcgt
    14401 gcgtgaagtg caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct
    14461 gcaaattatc atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg
    14521 tggattgggt acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca
    14581 gaccatgttt gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga
    14641 cgttcttagg gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg
    14701 ccgagctgga acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag
    14761 agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag
    14821 atgtttctgg tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag
    14881 ccgttgctga actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt
    14941 tctctggcga atatgacagc gccgactgct acctcgatat tcaggcgggg tctggcggta
    15001 cggaagcaca ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gctgaatcgc
    15061 gtggtttcaa aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg
    15121 tgacgatcaa aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc
    15181 accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg tcgccacaca tcgttcagct
    15241 ctgcgtttgt ttacccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc
    15301 tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat
    15361 ctgcggtgcg tattacccac atcccaaccg ggatcgtgac ccaatgccag aacgaccgtt
    15421 cccagcacaa gaacaaagac caggccatga agcagatgaa agcgaagctt tatgaactgg
    15481 agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga taacaaatcc gacatcggct
    15541 ggggcagcca gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgtaccg
    15601 gggtagaaac ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag
    15661 caagtttgaa agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg
    15721 acgcggtagt cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc
    15781 gcgagcaggg ggttgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc
    15841 acgcagaatt cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg
    15901 ttgctggccg catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg
    15961 atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca
    16021 acgagcagtt caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca
    16081 aaaccaaaac cggcgaactg tctatccact gcaccgagct gcgtctgctg accaaagcac
    16141 tgcgtccgct gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc
    16201 gttatttgga tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga
    16261 tcctctctgg tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga
    16321 tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc
    16381 tggatctcga catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg
    16441 gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac
    16501 gtcataaccc agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc
    16561 tgatcgagct gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg
    16621 aagtgaccta cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc
    16681 gtgaagcgat caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact
    16741 ctgcgaaagc gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc
    16801 gtatcgttac cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca
    16861 ttactgaata tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa
    16921 tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc
    16981 tgaatgacgc ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag
    17041 gtgacgacga agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac
    17101 cgccgacagc aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc
    17161 ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc ggtaaaataa gcattacgtt
    17221 atgctcacaa ccccggcaaa tgtcggggtt ttttaattgt gctgcgcgaa agcagacaac
    17281 aattttcttg cttcactatt ggctgcctgc atcaccatcc acggactgaa cgcccacggt
    17341 gtggcatcaa taccgtgtaa tacatctgct aaatcacacc attgataatc catcacttca
    17401 tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt
    17461 tccacaatgc cattcggatc ggtggcgcga tagcgaaagt caggatagat agattcagga
    17521 ggcgtaattt ccacgccaag cccataacgg caacggcgga tcaccgcgtc ttcgttgctt
    17581 tctcccagtt gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg
    17641 ctcagtgcgc ggcgggtaac taataattgt cctttggcat taaacagcca actggagaac
    17701 gcgcgatgta agcgggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga
    17761 actccctgtg cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc
    17821 tgatcactca tcattataga taattgaaga gtgaaatgat taaccaagtg agcgtttatc
    17881 gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca
    17941 tatatttgaa gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga
    18001 gagtaaaaag caacaacaga ctgaatatag cgcgcattaa aaaattcata ttattctcca
    18061 tgctcatttt tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa
    18121 tctttatcag agacggattc cttcaccagg tcagcatgat gctgatagcc attaaagaag
    18181 acattcagca caacggcgct aagggttgcg agcataatgc cgctatgcag caacggttgc
    18241 agtacggccg gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg
    18301 agactgatcg ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga
    18361 attcgaatcc ctgtcgccag taccatgccg aacatcacca gcccagcgcc gcccagcaca
    18421 aattgcggaa tggaggctac cagcaccgcc atttttggca ccatgccaaa caggattaat
    18481 ataattcccg aagaaataca cacccagcgg ctatgaacgc gcgtcacgct aaccaggcca
    18541 acgttttgag aaaatgacgt gtgggggaag ctgttaaacg tgccgcctat cattgtccct
    18601 acgccatcga cacgcagccc gcgaataata tcgtgtgaag agagtttacg accgactatt
    18661 tcacccagtg ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc
    18721 atggtcagaa tggaaacggg atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc
    18781 gcaaaccacg aggcatcatg tagcccggat aaattgactt cattcatcat ccacgaaagt
    18841 aaaaagccaa atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag
    18901 cgagtaatga gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta
    18961 ccatattgcg gattcccttt acctccggcg gcccagtcaa tacccacctg aatgatgcta
    19021 agcccaatag aagtaataac cacaccggta accagtggcg ggaataaagg catcaagcga
    19081 ccaataagtg gcgctaataa tgtggtgata aaacccgcgg ctatagtggc accaaatatc
    19141 cccagcaggc cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag
    19201 gtcaccgaca taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc
    19261 aataatgtga cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcttct
    19321 tttgagaggc ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg
    19381 acaaggacgt gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc
    19441 tcatcagttg ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc
    19501 agtgtttctc ctttcgcccg ccagagtaaa aagcaaccgt ttacgttaat gaaactgtct
    19561 aaacacatgc tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc
    19621 ggaggcaatc acataatcgt ggttaaacca cgaaaatgag aagagaacct gacaatgaaa
    19681 ttatctgtaa ttttctcata ttgatatttc attaatcatt ctggcatgtc gcactctcgc
    19741 atttaatcgt ttttatctgg atagcgctct tttggtcggc aatgttgaaa taatgaaagc
    19801 ggcatagatt gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat
    19861 gctacctaat atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa
    19921 tgaaagggat gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt ataggctgtc
    19981 atgcttgtga aattgcctgt gcggtggcac acaatcaaga aaactggccg ctgagtcaca
    20041 gtgactttcg accgcgtatc cacgttgtag ggaaaggcca ggctgcgaat ccggtggcct
    20101 gccatcactg caacaatgcc ccttgcgtta cggcttgtcc ggttaatgct ctgactttcc
    20161 agtccgatag cgtacaactg gacgagcaaa aatgtattgg ctgtaaaaga tgcgcaatcg
    20221 cttgcccctt tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc gacctttgta
    20281 accagcgcag ttccggcacg caagcctgta ttgaagtctg cccaacacag gcgttacgac
    20341 tgatggacga taaagggtta cagtggataa aggtggctcg ccagcgcaaa acggcagcag
    20401 gaaaagcgtc atcagatgct cagccatctc gcagtgcagc gttgctcccc gttaactcgc
    20461 gtaaaggcgc agataaaatt tcagcgagtg aacggaaaac ccactttggc gaaatatatt
    20521 gcgggctgga tccactgcaa gcgacttatg agagtgaccg ctgtgtttat tgtgccgaaa
    20581 aagctaactg caactggcat tgcccgctgc ataacgctat tccggattac atccgtctgg
    20641 tacaggaagg aaagattatt gaagcggcag aactttgcca ccagaccagt tccttaccag
    20701 aaatttgcgg cagggtatgt ccacaggacc gtctttgtga aggcgcatgt actttgaaag
    20761 atcactcggg cgcagtctct atcggtaatc tggaacgcta catcaccgat accgcgctgg
    20821 cgatgggctg gcgtcccgat gtcagcaaag ttgttccccg tagcgaaaaa gtggcggtga
    20881 ttggcgctgg gccagcaggg ttagggtgtg ctgatattct ggcgcgcgcg ggagttcagg
    20941 ttgatgtctt tgatcgccat ccagaaattg gcggtatgct gacttttggc attcctcctt
    21001 tcaaactcga taaaacggta ttaagccagc ggcgagagat attcaccgca atgggaatcg
    21061 actttcatct taactgtgaa attggccgcg atattacctt tagcgattta acttctgaat
    21121 atgatgcagt tttcatcggc gtggggactt acgggatgat gcgagcagat ctgccgcatg
    21181 aagatgcgcc cggtgtcatt caggctctac cgttcctgac tgcccatacc cgccagctca
    21241 tgggattgcc ggagtctgaa gagtatccgc tgacggacgt ggaaggtaag cgagtcgtgg
    21301 tattgggcgg tggcgatacg acaatggatt gtttgcggac ttccatccgc ctcaatgccg
    21361 ccagcgtgac ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg cgcaaagagg
    21421 tggttaatgc gcgcgaggaa ggtgtcgagt ttcagttcaa tgttcaaccg caatatatcg
    21481 cttgtgacga agatgggcgt ttaactgcgg tgggcctgat tcgtaccgcc atgggtgagc
    21541 cggggccgga tggtcgccgt cgtcctcgtc cggtagcggg ttcagagttt gaattgcccg
    21601 ccgatgttct gattatggcc tttggtttcc aggcgcatgc catgccgtgg ttgcagggca
    21661 gcggaattaa actcgataaa tggggcctga ttcaaaccgg tgacgtcggg tatttaccta
    21721 cccagacgca tctgaaaaaa gtctttgctg gtggtgatgc agttcatggc gcggatctgg
    21781 ttgtcactgc aatggccgca ggaaggcagg cggcgcgcga tatgttaact ctgtttgata
    21841 cgaaggcatc gtgatgaaat cgttaattat cgttaatccg gctgactgta ttggctgccg
    21901 tacttgtgaa gtagcctgtg tggtcgctca cccttcagaa caggagttga atgccgatgt
    21961 ctttttaccc cggctgaagg tacagcgact ggacagcatc agtgcgccgg tgatgtgcca
    22021 tcagtgtgaa aacgcccctt gtgttggcgc ttgccccgtg ggggcgctga cgatgggcga
    22081 gcaggtggtg cagaccaatt ctgcccgttg tattggctgt cagagttgcg tcagtgcgtg
    22141 cccgtttgga atgatcacca ttcagtcgtt gccgggggat acccgacaac aaattgtgaa
    22201 atgcgatctc tgtgaacagc gggaagaggg gccagcctgt gttgaatctt gcccgacaca
    22261 ggcgctgcag ttgcttactg aaagagaact caggcgagtc cgccagcagc gtattgttgc
    22321 cagcggtgag aatcctctgt gaaagaaatc tccgcgctga aaggcgcgga gaatctatta
    22381 gattgcccaa ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca ccgtaccaat
    22441 attcaacttt tgccattcgc gagcaaagac acggcctacg accagtgtca caaagcccag
    22501 cataataccg gtaacgatat tacaagtcag aacgatgaac acggcacaca ccaggccagc
    22561 cattgcgtca ataaaatcgt tgaaatccag cttcgagacg ttacttaaca tcagcaaacc
    22621 cacgtacatc agagcgggtg cagtggcgta accagggatc aaaaatgaca gcggtgataa
    22681 gaacagaatc agcaggaata acgcccccac tacggttgcg gtcaaccctg ttttaccccc
    22741 ggcggcggtt cctgccgctg attcgatata aaccgctgcg ggcgctgcgc ccaccagacc
    22801 ggagaatatt gaacttactg agtcactggt cagggctttg ccgccattga tgatctggtt
    22861 gtctttatcc aacaaattcg cctgaccggc gacggcacgg atggtgccag tggcgtcgaa
    22921 cactgcggtc atcaccaatg ccagtacact cggaagtacg gttggctgga gtgcgcccat
    22981 aatatcgagg ctgaaaatca gagacttacc atcttcgcca gtcagacttg gcatcgccac
    23041 cagaccgtgg tatttcaccg ctgggtcaaa gattaagccg ataatcgaaa ttgcgataat
    23101 caccaacaag atcccgccgg gtacgcggca cttctccaga ccgaagatga ccgccagccc
    23161 cagcaagctc atcatcaccg ggaaggaggt aaacgcaccg agcgccactg gcaagccttc
    23221 aatcgggttt ttgataacca tacccacacc gttagcagca atcagcagca gaaacaggcc
    23281 gataccgata cctgtaccgt gagcgatacc catcggtaaa ttacgtaaga tccaggtacg
    23341 cacaccggtt acggaaatag cggtgaagat gacgcccatc agaaagaccg cgcccagtgc
    23401 gacaggaacg ctaatttgtt gcccgagtac cagactgaat gcggtaaatg ccgtcaagga
    23461 aatcgcacaa ccaatcgcca ttggcaaatt ggcccacaac cccatcagca acgagccgaa
    23521 gcccgcgacc agacaggtgg caacaaacac agctgcggga ggaaaacctg ctttgcccag
    23581 cattcccgga acgacgataa cggaataaac catggccaga aaggtcgtta agccagccag
    23641 tacttcctga cgaacggtac tgccacgagc ggtaatttta aaataattat ccagcgcgcc
    23701 ctgtggcttt ggtgcgtccg gtgtttgtag gatgtctcca gacataatga tgtcctcacg
    23761 aggatgttaa agttttatta ttagttgcgt tcgtacacca gacgaccatc aacgtaggtg
    23821 cggtagatcg aacggtcatc gcccaacgtc atcatcacga acaatttgtc gactaaagaa
    23881 acagagttgt catagcgcag ctgctgtagc ggagtggcgg tgggttccat caccacgaaa
    23941 tcagcctctt tgccaggtaa aaagttgcca atcaaatcgt caaggcccag agatttcgct
    24001 ccgccgagcg tggccaggta aaacgcttca tatgccgaga ggcgatagcc ttgtaattgc
    24061 aatactttgt aggcttcgtt cagcgtttgc agcatgttga aagtggttcc ggcaccgata
    24121 tccgttccca tgcccacttt aactttcttc tgccatgctt ttttcaagtt gaataagccg
    24181 ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc tggatttggt ttcgctgaga
    24241 cgagcccact ctttttcttc gagatggacg cagtgagcaa agacacagtt tttaccggtc
    24301 aggccgtact gatggtaaac atccagataa ccatcatggt caggataaag cgatttcacc
    24361 caggcaattt catctttgtt ttcacagaga tgggtatgta cccacgtatc cggatattct
    24421 tctttcaggc gttgcgccat cgccatctgt tcaggagatg aggtcggggc gaagcgtggc
    24481 gtaatcgcat atagcagacg accatttttg tgccagcgtt caatcagttc tttgctttgg
    24541 tgatagctgc tttcggcagt gtcgagcaga taatccggtg cgttgcggtc catcatcacc
    24601 ttaccggcaa tcatacgcat attgatatga ctggcggctt caaacagcgc atcaacagat
    24661 tgcggatgaa cagtgccaaa caccagcgcc gtggtggttc cgttacgtaa aagctgcttg
    24721 atgaagaatg ccgacatttc gcgggcgtac tctaaatcct cataacgacg ttcagtaggg
    24781 aaggtgtgtt tattcaacca ctccagcaat tgttcaccat aggcccccac catttcactt
    24841 tgcggataat ggatatgtgt atcgacaaag cccggtacta tcagtttgcc gcgatagtcg
    24901 cgcacgcgaa tagtgtcagg aatttgatgc tttccgtctt cccattcgcc aaaccattcc
    24961 acttttccct gtttaatgag taataaacca tcctcaataa accgcagcgc agaggcaatc
    25021 tcttccgggt tatcgaccgt acgggtgaca tcaataaaac tgcctcgtac cgctttcaac
    25081 gtgtgttctc ctgacatcat tgactccttt actgttaatc catctcttcc gctgaggtaa
    25141 taccaggcag aacgttttcc tgtcggtagc caccagggag gataatattt aataaaatcg
    25201 cagttaaccc gccagcacaa atagggtttt caactagtac ataaatagag gctggcaata
    25261 ttttaaaaat ttcgggatcg taggagacgc caagtcctaa acctaaagaa gtggcgacaa
    25321 taagtgtttc tcggcgcttt aaaccgttgg tgatgattat gcgaatccca gcgatggcaa
    25381 tcatggaaaa catcaacgtc attgcgcctc ccagaactgc cgagggaatg gtcgtgaaga
    25441 agccgccaat catcggaaac aaaccgagga taaccagcat taccgcgatg gttcgcccga
    25501 cataacgtga agcgacgcca gtcatctgaa taaccccatt attttgcgca aacgtggtta
    25561 agggtaatga cccgacagcg gaggcgataa cagaaaccag accatctgcc agcacgccgc
    25621 ctttcagtcg ggactgatac tcttccccct gaatggggcg gcgggaaacc attgccgtgg
    25681 cggtgatatc gccgactgct tccagcacgc taagcagata aatcgtgcca accaccagga
    25741 actgatggaa gctaaaacta aagccgtatt tgaacggatg cgggatggtg attaacggca
    25801 aattgcgcat actgctgaaa tccaccatgc ccaggcataa cgatgcgata tagccgacac
    25861 atagcccaat ggcgatccct cccatgcgta gcaacggact gcgacagcag ttaaagccga
    25921 tcaccacaat taaaaccaat aaaccaacgc cgagatgttc gtaattgccg aacgtaccgc
    25981 tgcttttggc tgcaaatcct ccaccaaaat cgataatgcc gactttaatc aggcttaagc
    26041 cgatcatcag taccacaata ccgctgacgg taggcgtaat aacccgacgt aaatagggca
    26101 agataaatga agatccgaca accagaaatg cgccaacgaa ggagacgccg agaagcgacg
    26161 acatgattaa ctcttcgtga aaaccgtcgc ttttcatgct gctgcccagc gcaatcatga
    26221 ccgtaacaaa tgaaaaattg actgactgaa ttgagagtag gccagaaccg acgatgccgt
    26281 agcggtttac ttgtaaccag gtaccaatac cagaggcgat catcgccata gaaacaagat
    26341 aggcggttgt ttcagcggaa agctgtaagg ccgcacccac gattaacgcg ggggttacca
    26401 tcggaacgaa aattgccaac agatgggtaa tggcaccaac gagagcctga tgaaagggag
    26461 ggcgatcctc cagttcaaat ataaggtcag aacctgcatg gtttatatca gacatccctt
    26521 cactcctttt aaagtaaatt tgatcatctg aattagtaaa ataaattccc acgaatttgg
    26581 ggcgcaggac gccttgagaa cggcttatct cattttcgca gtcactattt agtcatcgtt
    26641 caaacagaga aacaggccgc cgaagcggcc atttattact tatatttttt ccagcgcggt
    26701 gagtattttc tccggtgtga aatgccattc gcgtaaccag atgccgcagg catcgtgaat
    26761 tgcggtagca atcgccggag ctgcgccatt tacaccaatt tccgagatcg atttcgcccc
    26821 gaacgggccg actttatcgt cgctcggcac caacacagcg cggaaatcac gcggaatgtc
    26881 accaattttc ggtgcgccgt aactgcgtaa atcacgcgtt aacgggtgac cttcggcgtt
    26941 ataaatgatc tcttcgctca tactgtggcc gatagcacgc agggtggcac cgtagatttg
    27001 tcccaacgct aactctggat tgaccggtgt accgcagtcc agcaaggcgt agaatttatc
    27061 caggcggatt tcacccgtac gcgtgttgac ggcaacttca gcgaagtttg cgccatacgg
    27121 gaaagcgaaa tcaggcgtga tataactgcc agttcccacc agtgaaccaa agccggtgcc
    27181 ggtttcgcct ttatgggcaa tatccccgaa actaacttcg cctttcttgc cgcgcacgac
    27241 gcccggcgtt gctagttgaa catctgccac tggctcaccc aacatttgcg cgccgtggaa
    27301 cagaattttc tcccgcagat tttccgctgc caaacgcgcc gcgttacccg agaagcaagt
    27361 accggacgag gcatatgcgc ctttatcaaa caacgcatga tcggtatcac cggagataac
    27421 atgcacgtcc tgcggtgggc agtgcagcac ttctgctgcc agtttcgtca ctacagtatc
    27481 cagaccagta ccaatatccg caccgccaga atgaacgata aaggtgccgt ccgattccag
    27541 tttgatcatg cagttagcct gatcgatatc cgggataccc gatttctgca tgataatggc
    27601 gacgccgcga ccgatgtgcc agtcactatt ttgcggtttc ggtgaagacc attggatcat
    27661 ctcgcgaccc tgacgcagga tctcttccag tgcgcagctg gcggcggaag gaacggaggt
    27721 cggcgcttta ccttcaccga ttgcaccgag aattttcagc tcttgccctt cgtgtacccg
    27781 gttacgttcg ataatttcca gttggtcgat ctgtaactgt tcagccagtt ccgctaatgc
    27841 catggtgata gcgaagttac ctttcggtgc gccataaccc tgataagcac catttgggca
    27901 aatgttgctg tagtaggtgg tgacctggaa atcgacgtta tcgcacggat ataacggcag
    27961 cgacagcgcc ggtccgttac acggtacggt gagtgagtgg ttgccgtaag ggccagtgtt
    28021 ggcgcggaaa tccatcttca ctgccgtcag gcgaccatct tttttcgctc ccagtttgac
    28081 ggtgactttc gcgacgtgac gagaggtgtt agcaataaac tcttcttcac gggtgtagcg
    28141 gaacagtacc ggacgcccgg tcacgcaggt tgcccatgcg cacacttctt ccagcaggat
    28201 gtcctgtttg gagccaaaac cgccgccaac tcgctcttta atgacatgaa ctttatgctg
    28261 tttcatgcct acgaggcgcg cgacctggcg gcgtaagtgc catggtacct gggtggaggc
    28321 gtggataacc agacgatcgc cgtccatccg ggtaaagcag atatgtgttt cagtcgggca
    28381 ctgctgtgcc tgcgttgagt tataggttcg ctcaatgatc acatcggcat cggcaaagcc
    28441 tttgtccata tcgccaatat gaccatgaat actggcggcg atatttttgc gagggcgaga
    28501 accgatcggg aagttgatga tcatatgctc gccgcgctgg gctgcatggc tgttatcgtc
    28561 ttccagagta tctggcgcac cagcaacata caccaccggt tcatcgtgca cgacaggcgc
    28621 atcttccgcc attgcttcgt cgatcgacat taccggctta agcacttcat attcaacgtc
    28681 gatgagcttc aatgcttcga gcgcaatttc ttcactttct gcgacgaccg cagcaacacg
    28741 atcgccgacg tgacgcattt tcttgccgaa catacggcgg tcaagcggtg acggttccgg
    28801 tgcgctctga ccacccgggg tatagtagat atccgggcaa ttcaggtgag taataacgtg
    28861 aacgacgccc ggtaaggctt cagctttgct gacatccaga tgagtaatca gtgcgtgagc
    28921 gtgtgggcta cgtaacattt taatgacgca ggcgtcagcc gttacgcggt cttcaacata
    28981 gcagggtttc gcctgcacca ttttcgcggc atcagtttta ggataatgct tgccaatgac
    29041 ttcaaggtcg tcacggaaag tcggagcgat atcaatggtg gcctgcggat tatttttacg
    29101 tgcaaccgcc agttcaatga cctgatagta ttgttgccag cctgcatcgc ggctgaacaa
    29161 accagaaagc gcgtcgtcga tctcttcgcg ggtaggtgcg gcgatgcgat cgagaagatc
    29221 ggtgataatc agagctgcag ctggatcgtt ataaccagac tgcaccacgc caacatcaac
    29281 cattgcctgt tgaaccagac ttaactcgtt ccatttgccc aaagattctg cggtacgaat
    29341 atctgccttc tctaactgtg cggcaataag caaggacgcg ttaacgatat taccgttaaa
    29401 gattattgcg tcagaaccgg caaacccgaa gccatcatca ctgttgcgta cagagtgcat
    29461 tcccatgtta aacaacagct tttgcacgtt ttcgcctgga ttaacggtta gctcctgagg
    29521 cgcgccattt aaagtaaagt ggatgatcat acggcttcct ccccagcctg ttggcagtcg
    29581 gcatacaggt cggctaccag aactcccgtg atatagcgtt tataggccac gctgccccgc
    29641 aaatcttcct gcgggaaaat ggcgttggcg acagcctgtt caagtgcatt gccttccaga
    29701 ttttgttttt cgacatcatg cagacgcagt gctttactgg cgacgccatc cagcgcaatt
    29761 cgcataccgt cgtggtctgt cattgcaacg gctgccgtca cgacggttaa acctgcctga
    29821 gaacggctaa ttttgcgggt cgcacaggtg cgatacggat ctttaatgat aatttcggtt
    29881 agcaggcgat cgcatgggca ggccaggtag tcctcgattg acagcgtttc gccgttgcca
    29941 aaaaccagtt cagcatccag tgccaacagg acgggaagca gcaccgactc ttcctggcgg
    30001 gcggcgattt caccaccaat ggtcgactga ttacgaacat ggcgtgagta aacaaaaccg
    30061 agggcttcac gcagcgctgc aggaataaat cgcgcatcac gcagtggctg caagcgagac
    30121 attgcgccaa tccgcagtgc accgttatcc cagtcaaccc agtccagttc cagatcctgt
    30181 aaggaaatgg caatcttttt atcggtacgg gttggtgtag cattgagttt gctgcccccg
    30241 gcgaaccaga cggcttcatc ctggtagcgg cgcttcagtt ccagcgcctg ttcgacagag
    30301 tcgggcctga aaaattgttc aatcatcttc tttccttatt taaccctctt ccctgcggga
    30361 agagggcatc tgtcatttat gccagcgcat ccatccgacg ccacatactg gcagcggctt
    30421 ttctggcttg tgcataaatg gaatcgcaat cgaagttaaa ctgacggtct tcatagacca
    30481 tcacaccatt gaccatcacg ctgtgaacgc tgcctgagcc cataccgaaa gcgatatgcc
    30541 cggcgatatt gtctgccagc agcggcgtcg gtgagttgta atcgcaaatg gttaaatcag
    30601 ctttgtaacc ggcttccaga agtccaaatt tcgcgccaaa gttgcggctc atcagttcgt
    30661 taccgttagt cagggctttg gcaaaactgt caggccacag cggaccaccc gcatcgcgat
    30721 gtttaaagaa ggcaaatttc atctcttcaa acatgtccga accaatgccg tccgttccca
    30781 acgccagatt gcggatgtcg ctaaggtgat ggttgtagcc gacatggttg ttcatgtttg
    30841 aacgggcgtt atgcaccagg aacgcatcgc gctgattgag tagggcgatg tcatctttcg
    30901 acaagtacag cccatgagcg accagcgttt tgctatcgat gagatcgaat tgcgctagtc
    30961 gtgccagcag gtctttgccg taccagtggt gactgtagga aacgtcgtaa aggtcttccg
    31021 cagcgtgaat atgcaaacca cggcctgtgg ctttcacggc ttcacgtagc atctccagac
    31081 cggcatctgg cacggtaaac ggcgcgtgag caccgatatg tgcttccacc agatacggct
    31141 cggttgctgc tttcttcgcc tcatcaatct gacgggcgaa gcggatgttt tcttctacac
    31201 cttcctgcaa ctctttgatg ccgttgttac ggtcagtagt ttcaaaacag gtcatcgcgc
    31261 gcaggccaac ttttaaaaat gcgtcgcgca atgtggagag cgacccgccg atatacgccg
    31321 gagaggcatg gtgatcgata accgatgtac atccgctctt aatcgcttcc agggaacaaa
    31381 tcagcccgct gtaatagagc gactcttcat caagggcgcg atcgagccgc caccagagat
    31441 ttttcagcgt tgagatgaaa tccgggcagg gggcgatgtt tgccataatt ccgcgggaaa
    31501 gccccgagta aaaatggttg tgcgagcaga caattcccgg catcacaatc cgaccatgca
    31561 tctctttgta gctggcatcg gggtagcgtt gcgtcagggc atcgccgata gcgacaatca
    31621 catcattttc gatagcgata tcaacgcctt cctgcacttt cgccgggtgt aactgcactg
    31681 cggtgacatt cttcagaatc aacatgatta cacctccacg cggcccagca gataatgatg
    31741 atgctggtgg acatggctga tgatgcggca catatcgttc agctccggtg gtacgttgtt
    31801 gaactgacct tcgctgtcga tgtttaacac ccagctttgg ttattcagtc gtactcgtac
    31861 ccggcaatct tccacaagga agcctgggtt gctgctgtta tcaaagtctt gcgccaggct
    31921 gaaaacggtg attttgtctt tgtatggttt accgttccac ggacagaact gagcgcagtt
    31981 gccgcattcg ttacagtaag cgtcgaggtg cagcgtctgg aatctattct ggaagcctgg
    32041 gaccgcaatg gagacgttgg cgcggttcgg gcagacatcc acacacttgc tgcaaacgta
    32101 gttacattcg aggcagcgag cggcttcctg ggcgacaaac gcgtcacgat cgtcactgtt
    32161 caccagagtg atagagatat cgcctttacg ttgatagatt tccgccggat tgacgttgtt
    32221 ccagtattta tcgttctggt gggaacggat attttcccga ctaaggatgg catcggtcgc
    32281 ccgacgcgcg gttccgacag cagcgacaat ggaggatggt ccgcgctgta cgtcgccgat
    32341 cataaagacg tcagtcagac gagtttcgcc attgtggtcg acgtctggcc agccgttttt
    32401 gtccagcggc acgcccatcg cattcagggc ttcagtatcc tgctgttcac caatggcggt
    32461 gatcaggctg tctacatgca gtgttactgt ttcgttggtt tcaaccggac ggcgacggcc
    32521 tttctcatcg ggttcgccaa gcgacataac gcgcaaggtt aaggtgccat cagcatcgaa
    32581 acgttccgga ttattcagga aacggaactc cacgccgtcg tgcaacgctt cttcatactc
    32641 ttcgcgccat gcgggcatct cttgcagtga acgacggtaa acgatcgttg ctttttctac
    32701 gcctggaacg cgtaacgccg cacgagcgca gtccattgcg gtgttacccg ccccgacaac
    32761 gaccacatgt ttgcccagct tgagcgctgt acccttgttg tattcacgca ggaaggggag
    32821 tgatttccaa acattttggt tgtcacccgc cagtttcaca ccgctatttt tatcagtgcc
    32881 ggtggcaatc agaacatagt ggaagccctg attttttaac tgttcaacgg tcaaatcggg
    32941 tgagcagcca tactcaaatt tcacgccgtg atcggcaaca aaatcgatat cgtgctgaat
    33001 taactcagcc ggaattcgga actgaggaat gatatttttc accacgccgc ccgcattggc
    33061 ttcgcgttca aacagcgtaa ccggatggcc cgctctggca aggaagtaac ctgctgccag
    33121 acccgccgga cctgcaccaa tcacggcaac cggatggcgt gaaccagaac cggctggttt
    33181 gtgccagcgt tgcttatatt catcccaacc tttttccagc gcgacttttt tcagttcgcg
    33241 gatattcagc gcactgtcgt aatccaggcg ggtacagttg tattggcact ggtgatcgca
    33301 aatatgaccg gtaatggcgg gcagagcgtt gcgttggtag atgagttcca gcgcgtcggc
    33361 atagcggtgt tcgccaagca gacggatgta ttccggaata tcttgcttga tagcgcaggc
    33421 agtaacacag ggggcaacgt agcaatcggt cagcggcagg tcttctgcca cttcaatacg
    33481 ctcttctggc ttccagtgtt tctgggtgta ttccatggtt aacgcatctg ctgccagtct
    33541 gttcagtcgt tcgacgtcaa catggtcaag tccccaggcg tcggagcctt ccagctcgcg
    33601 catgcaggca cttaagcgca aatagccgcc aggtttcagc aggtcggttg ccatagtaat
    33661 agggcgaata cctgtatcaa aaatatcgcg gatagtcagc tgactggcac caccggaata
    33721 agaaatgggc agtttgccgt caaaggcgcg agagagaact gctgcaacat tgatggagag
    33781 cgggaacagc gcacggcctg acatatacat ctcttcacca ggcagtgcgc ctttattgtt
    33841 gatggtgccg agagtgttag tcagttttac gccaaagccg agtgattttt cttttgccag
    33901 tgccatcagg cgttccagca tttccagtgc ttgcgtcagc ttgaggtcgt gatcaaatga
    33961 ctcttctttt aagcctatgt aaccgaaacc gcagacatcg aggatctcac gaacacgcgc
    34021 gtatcccagt aaggtcgggt taagtttcac aaaggtgttg agcccttttt cttccagcat
    34081 gtagcggcaa atggcttcga tttcatgcgg aggacagccg tgcatggtgg agagggtgac
    34141 gccctgcacc atactggtgg ggatgcgagc gggtaaggct tgcaagcttt cgcgtttttc
    34201 ctgcaatccg tggcgggcta aaaatgcgtc atcctggagt aatttattca gcgtatcgcg
    34261 atattgcgcg aatttcggat ggtcagatgc gtccatcata ttgtcgatga actgttgcat
    34321 tggcggttgc ttaatacctt cgaggttgta accgacgctc atattaaaga tgaacgattt
    34381 accggaatca gaaggctgga acatcgcttc gagaaggtgc agggcaaacc aggctttgag
    34441 gtattcatcc caggctttaa gcagggtaaa ttcggtagac cattcggtgt taaagcactc
    34501 gtcttcggca tcgatacagg gcttttccag ctccaggcgg tcaagaattt ggacggtttt
    34561 tagttcgatg aagcgtccgc cagtcagcca ggaagtgaca atattttgcg cgagctgcgt
    34621 gtgcgggcca gcggcagggc cgacgggagt ggcacaggtt tcaccgaaga cgctaacagt
    34681 tttacctttt acaggtgagt aaaactgttg ctcgggaata ccaaagattg agcgttgttg
    34741 ttggtattca tcaaatatgc gcgtcaaaag ttcctcaaac ggaatgggac gcataatatc
    34801 ccccataacc ctctccttag cttatacaga tatcattgag tgaccggtgt tgagaaatcc
    34861 ggtggtttag ggggatagag caacaatcgc accactcttg ccaggtttgg cgggaaatat
    34921 aaaaaaatgt gaggcggctc gaaacttaaa ggctttttgt gaatgaactc acaggtgcca
    34981 aatgagaatt aaaatcactc ctgaaaatta ttattgtcag cccatttatc actttt
//
LOCUS       DAZFRJ010000028        34954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000028 DAZFRJ010000000
VERSION     DAZFRJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.028"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..162)
                     /locus_tag="ACHKUN_001253"
     CDS             complement(<1..162)
                     /locus_tag="ACHKUN_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HFO6659551.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQK"
     gene            complement(422..1675)
                     /gene="amiC"
                     /locus_tag="ACHKUN_001254"
     CDS             complement(422..1675)
                     /gene="amiC"
                     /locus_tag="ACHKUN_001254"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO6659552.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1907..3238
                     /gene="argA"
                     /locus_tag="ACHKUN_001255"
     CDS             1907..3238
                     /gene="argA"
                     /locus_tag="ACHKUN_001255"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO6659553.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3300..5126)
                     /gene="recD"
                     /locus_tag="ACHKUN_001256"
     CDS             complement(3300..5126)
                     /gene="recD"
                     /locus_tag="ACHKUN_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO6659554.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5126..8668)
                     /gene="recB"
                     /locus_tag="ACHKUN_001257"
     CDS             complement(5126..8668)
                     /gene="recB"
                     /locus_tag="ACHKUN_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO6659555.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEELELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8661..11549)
                     /gene="ptrA"
                     /locus_tag="ACHKUN_001258"
     CDS             complement(8661..11549)
                     /gene="ptrA"
                     /locus_tag="ACHKUN_001258"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO6659556.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11725..15093)
                     /gene="recC"
                     /locus_tag="ACHKUN_001259"
     CDS             complement(11725..15093)
                     /gene="recC"
                     /locus_tag="ACHKUN_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO6659557.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15106..15429)
                     /gene="ppdC"
                     /locus_tag="ACHKUN_001260"
     CDS             complement(15106..15429)
                     /gene="ppdC"
                     /locus_tag="ACHKUN_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO6659558.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15414..15821)
                     /gene="ygdB"
                     /locus_tag="ACHKUN_001261"
     CDS             complement(15414..15821)
                     /gene="ygdB"
                     /locus_tag="ACHKUN_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO6659559.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15818..16381)
                     /gene="ppdB"
                     /locus_tag="ACHKUN_001262"
     CDS             complement(15818..16381)
                     /gene="ppdB"
                     /locus_tag="ACHKUN_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6659560.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16372..16842)
                     /gene="ppdA"
                     /locus_tag="ACHKUN_001263"
     CDS             complement(16372..16842)
                     /gene="ppdA"
                     /locus_tag="ACHKUN_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6659561.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17026..17820)
                     /gene="thyA"
                     /locus_tag="ACHKUN_001264"
     CDS             complement(17026..17820)
                     /gene="thyA"
                     /locus_tag="ACHKUN_001264"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO6659562.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17827..18702)
                     /gene="lgt"
                     /locus_tag="ACHKUN_001265"
     CDS             complement(17827..18702)
                     /gene="lgt"
                     /locus_tag="ACHKUN_001265"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO6659563.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18853..21099)
                     /gene="ptsP"
                     /locus_tag="ACHKUN_001266"
     CDS             complement(18853..21099)
                     /gene="ptsP"
                     /locus_tag="ACHKUN_001266"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6659564.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21112..21642)
                     /gene="rppH"
                     /locus_tag="ACHKUN_001267"
     CDS             complement(21112..21642)
                     /gene="rppH"
                     /locus_tag="ACHKUN_001267"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO6659565.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21995..22141)
                     /gene="ygdT"
                     /locus_tag="ACHKUN_001268"
     CDS             complement(21995..22141)
                     /gene="ygdT"
                     /locus_tag="ACHKUN_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO6659566.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22327..23016
                     /gene="mutH"
                     /locus_tag="ACHKUN_001269"
     CDS             22327..23016
                     /gene="mutH"
                     /locus_tag="ACHKUN_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO6659567.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23085..23798
                     /gene="ygdQ"
                     /locus_tag="ACHKUN_001270"
     CDS             23085..23798
                     /gene="ygdQ"
                     /locus_tag="ACHKUN_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6659568.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23936..24154
                     /gene="ygdR"
                     /locus_tag="ACHKUN_001271"
     CDS             23936..24154
                     /gene="ygdR"
                     /locus_tag="ACHKUN_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO6659569.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24262..25302
                     /gene="tas"
                     /locus_tag="ACHKUN_001272"
     CDS             24262..25302
                     /gene="tas"
                     /locus_tag="ACHKUN_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO6659570.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25334..26527)
                     /gene="lplT"
                     /locus_tag="ACHKUN_001273"
     CDS             complement(25334..26527)
                     /gene="lplT"
                     /locus_tag="ACHKUN_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO6659571.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26520..28679)
                     /gene="aas"
                     /locus_tag="ACHKUN_001274"
     CDS             complement(26520..28679)
                     /gene="aas"
                     /locus_tag="ACHKUN_001274"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO6659572.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29265..30296
                     /gene="galR"
                     /locus_tag="ACHKUN_001275"
     CDS             29265..30296
                     /gene="galR"
                     /locus_tag="ACHKUN_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO6659573.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30303..31565)
                     /gene="lysA"
                     /locus_tag="ACHKUN_001276"
     CDS             complement(30303..31565)
                     /gene="lysA"
                     /locus_tag="ACHKUN_001276"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO6659574.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31687..32622
                     /gene="lysR"
                     /locus_tag="ACHKUN_001277"
     CDS             31687..32622
                     /gene="lysR"
                     /locus_tag="ACHKUN_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO6659575.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32609..33301)
                     /gene="ygeA"
                     /locus_tag="ACHKUN_001278"
     CDS             complement(32609..33301)
                     /gene="ygeA"
                     /locus_tag="ACHKUN_001278"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO6659576.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33430..34848)
                     /gene="araE"
                     /locus_tag="ACHKUN_001279"
     CDS             complement(33430..34848)
                     /gene="araE"
                     /locus_tag="ACHKUN_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO6659577.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8581 a         9592 c         8621 g         8160 t
ORIGIN      
        1 tttttgctca tttctgacat aaaaaatccg gtttgcagga tgctcaggga ccgacatacc
       61 atatttttta ttgtaaccaa cgccaggccc agaaatgata acctcctgtc cgttcaccga
      121 tgcctgtaca caattattgt tcatgacttt ttcgatgatc attccatggc tcccaaaaaa
      181 aaaggcaagg catgtcagaa gtaataatta cttcaaacat tgccttgcct gatttaacag
      241 ttacaagata attaactaat gcgtatacct tacacggaat tgtgcttaat aaaagagatg
      301 acaggcttat tttttaagcc gatattcaaa tattcgacag cagtcacaaa accatcgttc
      361 aacatttatg taaataatat ttattcaatg aaacataaat taattgaata aattaaaaac
      421 atcatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatc cccgcaagaa
      481 tagactccgc aacttcctgc tgaaaggtcg ccgttttcag tttacgctct tcctcaacgt
      541 tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt agtacggcaa
      601 acccggcctg ttcaacctga tttttatgca gcttgttgat tttaccgagc ttattcagta
      661 ccgctttacc aaacttaagg ctgtcagcaa tggtcagcga ctgcaccata tcgaacatgg
      721 tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct
      781 gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag agcgcaaaca
      841 cagaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag acaaacaggt
      901 cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat
      961 tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc aaacggcgag
     1021 ctatttgcaa taccacatct ttttctcgcg ttttgtattt ccccaccgca ccggagtctt
     1081 cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct gctttacccg
     1141 gttgtggacc actttgtgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct
     1201 ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca
     1261 ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt
     1321 gctttaattc aaaaaccatc cgtacggtct gcgggtcaaa ttgcccgacg cgcgccgact
     1381 tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt
     1441 ttacatcttc gatatccacc accacgcgtt ccgggttact caacgcgaac tgcttatatt
     1501 tcagctgacg atttgattct accgtcacac gggtgtagct ggacgcaggc cagacgcgca
     1561 ccgccacgac ctggctgacc gcagccagac tgacctgact tacgctcaat agccacatgg
     1621 cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc
     1681 tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt
     1741 tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg
     1801 cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca
     1861 gaataaaaat acactaattt cgaataatca tgcaaagggg tgtgccgtgg taaaggaacg
     1921 taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg
     1981 aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat
     2041 cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc
     2101 acgtccgcag atcgacgcaa atctggccgc gcatcaccac gaaccgctgt atcacaagaa
     2161 tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca
     2221 actggacatc actgctcgcc tgtcgatgag tctcaataac acgccgctac agggcgcgca
     2281 tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg atgacggcgt
     2341 ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact
     2401 ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt
     2461 taatctgacc tcggaagaga ttgccactca actggccatc aaactgaaag ctgaaaagat
     2521 gattggtttt tgctcttccc aaggcgtcac taatgacgac ggtgatattg tctccgaact
     2581 tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg attacaactc
     2641 cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg
     2701 tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg
     2761 tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga
     2821 tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg
     2881 ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc gcgataacac
     2941 gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg
     3001 tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat
     3061 tgccgctcag gcgaagcaga gcggcttaag caaattattt gtgctgacca cgcgcagtat
     3121 tcactggttc caggaacgtg gatttacccc agtggatatt gatttactgc ccgagagcaa
     3181 aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt tggggtaagc
     3241 gatgaaaatc gccggatgcg acatgcgtaa cactcgtgcg tcgcatcagg caattacgtt
     3301 tattcccgcg aactaaacaa tgccgccaga ccactgcgcc gctcagtgcg cgtggcgatt
     3361 gccgcactta atatgcgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcact
     3421 gcggtgtaaa ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc
     3481 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt cgtttcgtgc
     3541 tctggcaggc ggctcggttg cacagactta atattgccgt ccggcatcgc aaaccaaacg
     3601 cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg
     3661 ctgtcattac gggcaatcat caccggtcga ccttcgtacc aacgagagtg cggatgacga
     3721 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
     3781 aacggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg aatgattaaa
     3841 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc aagagcttcc
     3901 tcaagcatcg caatataatc ttctccgctc tgtaaaagtc gtttttcgat atcagtaaaa
     3961 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac
     4021 tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg
     4081 cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt caggcggctt
     4141 agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat atcgcccagc
     4201 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
     4261 tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca
     4321 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
     4381 cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt
     4441 tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg
     4501 gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga
     4561 attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg gccgccggaa
     4621 accaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca gttaatttca
     4681 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
     4741 gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca catgcgattc
     4801 aaataaagac gatcgccaca gaggaccatc ggcgtgggtt catctcccct gctgaccgct
     4861 tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
     4921 gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt
     4981 ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct
     5041 cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg ctccacagct
     5101 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
     5161 catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat
     5221 gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga cgatcatagt
     5281 cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa agctgatatt
     5341 gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg taagccgaac
     5401 tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
     5461 ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc agcggcgggc
     5521 agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
     5581 gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac
     5641 tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca
     5701 acaccggttc ccactgcgat tcaaagccgc cgagttccag ttcttcctgt acccagttcg
     5761 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
     5821 acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc
     5881 ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct
     5941 gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agcgtcctcg
     6001 cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca ccagtttgtg
     6061 ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg
     6121 catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt
     6181 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
     6241 ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg
     6301 caagctcgac gctttctggc gcagcgttaa gatccagaac agcctcaaac gagtggcgat
     6361 cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg
     6421 gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt ttatcacttt
     6481 cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
     6541 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
     6601 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa
     6661 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
     6721 gccgataacc atcgaactct tcaactaccg catcccacgc atgttcgtca ttattcagcg
     6781 tttcgatatc cagcgcgttc agccccatca ttgacgttgc cagcgcactg cgcagggtgt
     6841 tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag
     6901 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg
     6961 catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
     7021 gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
     7081 gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgataa tcgccaacgc
     7141 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaaca
     7201 caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa
     7261 acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc attccaggtg
     7321 cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc gccttcatat
     7381 aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg ccaatcagca
     7441 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
     7501 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
     7561 acacctcacc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
     7621 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
     7681 gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa
     7741 acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag aaacgctggg
     7801 agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct gcccaggcgc
     7861 tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
     7921 aagattcgat cagcgcatca agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat
     7981 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg
     8041 ctttgataac cggtgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
     8101 gccctttcca ggtttcaaag accaccaggg caatttcacg cggcagcggg tagcagtggc
     8161 gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct
     8221 gctcaaacag cataccggat tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt
     8281 gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg
     8341 cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg tcggtggttt
     8401 cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgacca cgcaattctg
     8461 ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa
     8521 aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
     8581 ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag
     8641 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
     8701 aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg
     8761 ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac caccgcctga
     8821 tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca
     8881 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
     8941 ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac
     9001 tcttctggct tcatcgcccg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
     9061 tcccacaaga aggaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
     9121 cgccccacgc tcattggaaa tgcaaacacg gcatagccca attgttcttc ggtacgcaac
     9181 tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg
     9241 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttacca
     9301 gctttttcaa agatgacgga ttgtttttta tcgaccacga catctttgtt acgacaccac
     9361 tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctggtcc
     9421 tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga
     9481 taggccagca cctctttcaa cgtaatggag ggcaaaattt tgcgccgttc atctcgcgag
     9541 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcgaa cgctttgccc
     9601 ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc
     9661 gtggcggtat agctaaagta gccttccagc aatgcctgga acagctgcgg cagatgctgg
     9721 gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa acttatgcca
     9781 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
     9841 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
     9901 acatcggctt ttggctcgct ggcaaaataa cggcttggcg catacaccac gcgcagattc
     9961 gactcatcaa caatcagctc tggatggtca tatttcttgt ctgacttaat cagcgagaaa
    10021 tcgtcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc ggcttttttc
    10081 tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc atcgacaaag
    10141 taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc
    10201 gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc aatattgact
    10261 gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa
    10321 tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt cgccagttca
    10381 tcgaagtatt gtttatcgat ccctttttca cgtaacaaat tgaggtagct aaaaatagcc
    10441 gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga gatcgctaat
    10501 acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc
    10561 tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag ataggtaatc
    10621 aattcatcgg ttttgctacg gaatttcgcc gagttgttat caatacgaaa ctctacgcgc
    10681 aacactttac gcggcagcgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    10741 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
    10801 tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac cgccttcatc
    10861 agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca
    10921 ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg
    10981 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    11041 aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag caagggttct
    11101 gcaatagcat cagccaggcg gtctaccgca ccaggcaagg cgtcgttctc aacttccaga
    11161 tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga
    11221 tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca
    11281 aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcaccacc
    11341 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta
    11401 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    11461 atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac taacaacaat
    11521 aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact taattttctg
    11581 tccagattca acacgttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt
    11641 cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag taagcggata
    11701 gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg gtaacaggaa
    11761 acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc gccagagcct
    11821 ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
    11881 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
    11941 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
    12001 catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
    12061 aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc
    12121 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
    12181 taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat
    12241 ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc gacaggcaat
    12301 gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa
    12361 agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc
    12421 ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa gcccttccag
    12481 aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca cctgcaaacg
    12541 catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
    12601 ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg
    12661 gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg
    12721 cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct
    12781 ttcatcacag ttgagcgctt catcgtccgg tagataatga ctttgcccga tgtagtcgat
    12841 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat
    12901 atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca ggtagcggtc
    12961 gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa
    13021 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
    13081 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg
    13141 gctgatacgt tcttgatcca gacgctgcgc cagttcatca cgcaatagtg acagcggcac
    13201 cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
    13261 cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
    13321 acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt
    13381 tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc
    13441 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa
    13501 catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg
    13561 aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact ggcgtaaata
    13621 acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagtaatgc
    13681 cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga taaacgcttc
    13741 cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc
    13801 cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac
    13861 catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg ccagcaggcg
    13921 atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct
    13981 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
    14041 agcaacggca cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc
    14101 tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag aaaggagata
    14161 aatgtagtcg cgcccaagct taccccatga agccagcagc gggttgccga catcctgttc
    14221 accatcgctg ttaaagagct gcccggcatt ttcgctatca cgaaatagcg gcaattcgcg
    14281 atcttcaaca ctgtgtcgac gctgacgggt cagcagtttc gccagataag caggatcttt
    14341 aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
    14401 acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
    14461 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
    14521 ctgatagaga ttggcgcggt gccaacgcgg ttgtccgagt tcatgggtat attccaccag
    14581 cgccttccac aacggcgctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc
    14641 tgtttcccac tgtgccagcc actccgggcg atagaccaga tactggtcaa acaggtccgc
    14701 cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagcca gataatgccg
    14761 caacagggta aagtcttcgc gatccagcaa ttgcggcagc agagtcatca gtttccagct
    14821 catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc ggacgaacat
    14881 atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg
    14941 cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa tcatctctgg
    15001 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
    15061 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
    15121 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
    15181 gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg caggtggcga
    15241 aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt actggtttct
    15301 gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga caatcatcac
    15361 catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat tcttcaggga
    15421 agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa
    15481 acaatgtttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccggca
    15541 accaataagg cttcattatc tgccagtaaa cgcaaacaaa cccgggcatc ggttccagcg
    15601 tactgcgagc actgaactgc gggctgcgtc tgccaggagt gcattttccc ccacgccagc
    15661 gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaggca
    15721 aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac cagcagcatc
    15781 aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat cctgtcacgc
    15841 tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc atattaaccg
    15901 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    15961 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    16021 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcagactct tttaccggtt
    16081 cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag tcaccctgtc
    16141 cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc ctctggagat
    16201 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgc
    16261 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc
    16321 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca
    16381 tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccaccagcca
    16441 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    16501 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gtggcacaaa
    16561 gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca caaggcacca
    16621 taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt tggcatcttc
    16681 acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccatagcc gttgcgactg
    16741 ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa tcagcatcgc
    16801 gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga
    16861 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    16921 ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac ggagggtaaa
    16981 aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga tagccaccgg
    17041 cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga aacggtagtc
    17101 gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg gttcgcggct
    17161 taattgcaga tgagtttgat ccatatgatt gctgtacagg tgcgtgtcgc cccccgtcca
    17221 gacaaaatcg cccacttcca gatcacactg ctgcgccatc atatgcacca gtaacgcgta
    17281 gctggcaatg ttaaacggca ggccgaggaa aacgtcacag gagcgctgat aaagctggca
    17341 agagagtttg ccgtctgcca catagaactg gaagaacgca tggcacggtg ccagcgccat
    17401 tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat ccgggtcgtt
    17461 tttcagctgg ttcagtaccg tagtgatctg gtcaatatga cgaccatccg gcgttggcca
    17521 ggcgcgccac tgtttaccat acactggccc gaggtcgccg ttttcatcgg cccattcgtc
    17581 ccagatggtg acattgtttt cgtgtagata agcaatgtta gtgtcgccct gcagaaacca
    17641 cagcagttca tggatgatgg aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc
    17701 atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg ttccggtacg
    17761 gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat actgtttcat
    17821 ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca tcatgatcac
    17881 acccgcgaca atcatcggga tggaaagaat ttgtcccatg ctgatgtact gcacccaggc
    17941 accagtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa acgcgccgta
    18001 accaatcagg aacaaacctg agacagctcc cattgggcgc ggtttacgaa tatacaggtt
    18061 gaggataata aacagcacta caccttccag caacagctcg taaagctgtg atgggtggcg
    18121 cggcagcaca ccgtaagtat cgaaaatgga ttgccactgc gggttggttt gcagcagcaa
    18181 aatatcttct gtacgggagc cagggaacag catggcaaac gggaagttcg ggtcaacgcg
    18241 gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac caaacggaat
    18301 gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc gggcgaagat
    18361 aatcatcacc acgataacgc caatcaggcc gccgtggaag gacatgccgc cgtcccagac
    18421 acggaacaga tacagcggat cggccataaa ctgcgggaag ttgtagaaca gaacataacc
    18481 aatacgtcca ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt caacttcatt
    18541 tttggtccag ccactgcccg gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat
    18601 gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat
    18661 gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc acaagttctt
    18721 gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa
    18781 aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta aaagatatgt
    18841 atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccacgac gctccataaa
    18901 ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct tccagactac gctgcgcaag
    18961 attttctgct tcggcataat caatgcgccg cagcaggtat tttacccgcg ctacagaacg
    19021 tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc acatgggatc
    19081 gcccgccatt tcaccgcaca aacggagatc gattccatgt atttccgctt cccgggcgat
    19141 catcgccaga gctcgtaaca ttgcaggatg aagactgtca taaatgttcg ccacccgggt
    19201 attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa cagagatgaa
    19261 atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt ccagcatgat
    19321 gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac gtccggcacg
    19381 ttcaatcagg cggcgtgctt catcaacttc atcgaggctt gtgaccatcg gcaacagaat
    19441 attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga tcaagaagat
    19501 ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat tctcttcgct
    19561 gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca aggtgacggg
    19621 tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt cttccgacgg
    19681 gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc catcaatacg
    19741 gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga cttttatacg
    19801 ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac ggctaagctc
    19861 aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg gatcgaccag
    19921 caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg aaggctgaat
    19981 atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag catgggagtt
    20041 ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct cagcaagagt
    20101 tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg
    20161 attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat cgccagcccg
    20221 ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt caatgaccgt
    20281 ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag
    20341 ccgggtgtcc gaaagtaggt gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc
    20401 tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc
    20461 ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca cctgttccat
    20521 taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa
    20581 tgcgcggatt cgcgtctggc gatattgccc aaacagggca gtcaactgcg actgggaaag
    20641 aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt cgtcatactg
    20701 gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt gaataattgg
    20761 tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt tgaagctggg
    20821 gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca ggccgacgat
    20881 cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc
    20941 catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga cctcggtatc
    21001 catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc gtggtgcgct
    21061 ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga cttaacctct
    21121 tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca gtgacatcac
    21181 cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg acaccacctg
    21241 tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg tactgctggt
    21301 ttgcatattg atttctgcat cgccgcttac cagctgcaag agaaaccatt tttgtttttg
    21361 gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa
    21421 ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta cttcttcaaa
    21481 caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc cttgcggaaa
    21541 ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc gattacaaat
    21601 cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac ctcaaaataa
    21661 agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac tctctgcagg
    21721 gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta taaacagagg
    21781 aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga agaactacgg
    21841 aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat tttaaaatat
    21901 tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc
    21961 agctcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat ttatccacag
    22021 aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcgtcg cacgtcaagg
    22081 ctgtaaatgg aaacagtagt ggaggttttt cacagttatc ccagctttct gtggataaca
    22141 tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg cagtgttgcg
    22201 caactcgatt accggcagcc taaaaagcaa tataaatcag agaattgaac aacacatatg
    22261 gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta accaaggtat
    22321 catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga acagttgtta
    22381 gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact tgccgggctg
    22441 gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact ggagatctgg
    22501 ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg cgtggaactt
    22561 aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc
    22621 ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca caagctcaaa
    22681 cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta
    22741 ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg cgaagactgg
    22801 gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc tcgtcacggg
    22861 gaatatttac agataagacc gaaagcagcg aatgcgaaag cgcttaccga agccattggt
    22921 gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa gaatttcacc
    22981 agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc tgccgctttc
    23041 cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta tttgcatgga
    23101 taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag atcgttcttg
    23161 ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg
    23221 ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg ctgctggcat
    23281 caatcgcctg ggttacgcgc ctgacaaatc cgctttttac aatattcagt caggaaattt
    23341 ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa gccagcaagg
    23401 aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc
    23461 tcggcgctat cgtgcagatt atgctgctgg atattatctt tagcctcgac tcggtgatta
    23521 cggctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg attgccgtag
    23581 gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat ccttcggtaa
    23641 aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg gaaagtttcg
    23701 acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt gcggttgaaa
    23761 gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg
    23821 gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt caagatatat
    23881 aacgtcggtt tataaacaga ctattatcat aggtaggcca aatgaggata accggatgaa
    23941 aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg ggtgttccag
    24001 tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa aacctgaaat
    24061 tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa
    24121 ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat ccggctggcc
    24181 ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc cttatgtcgt
    24241 gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc gctggaagtc
    24301 agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc cgacgcccac
    24361 gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc cgaaatgtac
    24421 ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg
    24481 gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc
    24541 aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat ccgcgaagcg
    24601 ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca ggtgcactgg
    24661 ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga ttctgcgcct
    24721 gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc gggaaaaatt
    24781 cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct gcatctggcg
    24841 gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct gttaaaccgc
    24901 agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact gctggcctat
    24961 tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa acccgctggc
    25021 gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc
    25081 gtcgcggcgt atgttgatat cgccagacgt catggcctgg accctgctca gatggcgctc
    25141 gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac cacgatggat
    25201 cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt attagctgaa
    25261 attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg ccggttaaaa
    25321 ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt gccagcgcaa
    25381 acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctatcatt accgccagcg
    25441 agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt actgcaatcg
    25501 cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg agcggaacga
    25561 caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc agcagctcgt
    25621 gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc atacagcgtg
    25681 acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg ataccaatcg
    25741 ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc gccaccggta
    25801 cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa ctggtgccca
    25861 ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc aggaaactgc
    25921 gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt ttgggaatgt
    25981 agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg gcgacgagga
    26041 catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc gctatggtag
    26101 aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat tcgccgagaa
    26161 taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta tagccgagaa
    26221 acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg ttggcaaaca
    26281 tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc gcaaaaagaa
    26341 tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga tagaactgcg
    26401 ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca gagagaaact
    26461 gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg tgcactgact
    26521 cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg
    26581 ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc
    26641 ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc gttatctgtg
    26701 gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt ggcatggact
    26761 ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac catttcgcct
    26821 gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa
    26881 cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac attctcggcg
    26941 gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat tatgttcggc
    27001 cctttcagtt gcaggcgtcc gccctcttcg ataccaggaa ccgacaacag gcgcgcatcc
    27061 attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac gttgatagaa
    27121 acgacaggcg cgcactcggt tacaccatag ccttcaagga tgcgcaggcc aaatttatcc
    27181 tgccaaagct gtttggtact ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga
    27241 cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg
    27301 aacaacacgg tgcaactgcg gtcatacacc aactccggca caatgcggta atgcagcggg
    27361 cttggataaa ggaacacttc tgcacctgta agcagtggcg taaacaggcc taccgtcagc
    27421 ccaaaggagt gaaacagcgg taatgccgac ataaagcgat cgttggtggt gaagtcggca
    27481 atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac gacgcctttt
    27541 ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt
    27601 acctgcgcca ggcgcggcat cagcaaatga gcgaagatcc atactttgtc ggcagtggtg
    27661 acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg ctccggcaga
    27721 tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt gatttcagcc
    27781 gccgtaatag cactggtcag cccttttacc ccagcagtgt agttcatcat tgcgggaatg
    27841 cggcgacgcg cgatggcccc aaaaatcact gccgcactga tgcctgcatt gggcagcatt
    27901 aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac aaacagcgtt
    27961 ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac acatttcttc
    28021 ccggctccga agcggtacat tgcactcagt aaagattcgt acagagtttc acgcgggcgc
    28081 accgccatac gcgcttccat cattatttga tgcagcattt cgccagcgat tttgcgacgg
    28141 tcacgggcac gcggcgcatc cggcatcgcc acctgcgttg gtggcaaaat atgcagagta
    28201 atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc
    28261 gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac aaaacccgcg
    28321 ccatcgtaga ttttcatcag cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc
    28381 actggtcggc cctgttccac cagacgtacc agatgtttaa tagccatagg ttgcgtcggg
    28441 tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg ttggcttatt
    28501 gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca
    28561 ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca
    28621 cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt
    28681 ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac gagataaaga
    28741 acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca
    28801 agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc tgggatcttg
    28861 attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg aagggaaatg
    28921 caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa aaaaaaacct
    28981 gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact tcacctatca
    29041 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    29101 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    29161 cgtttggctc gcattcccac gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc
    29221 gtaacactga aagaatgtaa gcgtttacct actaaggtat tttcatggcg accataaagg
    29281 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    29341 ctaaagccag tgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    29401 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    29461 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    29521 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    29581 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    29641 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    29701 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    29761 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    29821 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    29881 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    29941 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    30001 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    30061 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    30121 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    30181 tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg ctggtacgtc
    30241 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    30301 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    30361 gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta gttggatgac
    30421 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc cttcacttcc
    30481 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    30541 cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    30601 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    30661 aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct ccgcacctga
    30721 gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc cagtttcaca
    30781 gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc ataataatgt
    30841 tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc gcccgcagaa
    30901 atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc acacacctgt
    30961 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaaa
    31021 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    31081 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cgggttaacg
    31141 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    31201 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    31261 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    31321 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaatcgc
    31381 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    31441 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    31501 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    31561 ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata aaaagaataa
    31621 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    31681 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    31741 ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    31801 ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg tggacgatta
    31861 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    31921 gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    31981 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    32041 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    32101 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
    32161 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    32221 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat cagcctttcc
    32281 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    32341 cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg ggcgggggta
    32401 ggtgtttcgg tggttaaccc gctcaccgca ctggattatg cggcaagcgg tttagtggta
    32461 cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct gcaccgcccg
    32521 tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc gaaactggtc
    32581 acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
    32641 catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga accaataaac
    32701 caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg cgagcaatca
    32761 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    32821 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    32881 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    32941 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    33001 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    33061 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    33121 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    33181 gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    33241 gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
    33301 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    33361 caacggccag gtgcccgatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    33421 cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    33481 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtgatgc ccacaaacgc
    33541 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctat caagcagtgt
    33601 caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg aacaggtaat
    33661 accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca ccactggcgc
    33721 ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga gccaggacaa
    33781 gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca ccagagtgcc
    33841 taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt tatctaccgt
    33901 aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag tcgcaatcat
    33961 ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcat agtacatgat
    34021 gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa acacagcacg
    34081 acggacgtta cggttgatct taaacagtgc ccaaccgcct tgttttaact tcaggctttc
    34141 acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac
    34201 ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat ttggcaggaa
    34261 gaccaccaga ataatcagca ggactgctgg taaagcaaga acccccaaca ttgcgcgcca
    34321 gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac
    34381 catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca tttcagaaag
    34441 atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca cacgagcggc
    34501 gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaaccgagta caaacaggat
    34561 ggcccccgcc atcaggctgt atttacgacc caggcggaac gacagccaac cattaaacag
    34621 cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca aacgactggt
    34681 cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga tatcaagacc
    34741 aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac gccgcgtatc
    34801 ccgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt tttcctgcca
    34861 gcagagagta agacatagtg aaaaaatacg tgaacaactc acgcaggtgt caggtcggaa
    34921 acagcataaa tatggattaa attgctgcga catg
//
LOCUS       DAZFRJ010000029        34148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000029 DAZFRJ010000000
VERSION     DAZFRJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.029"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..898)
                     /gene="tcyN"
                     /locus_tag="ACHKUN_001280"
     CDS             complement(146..898)
                     /gene="tcyN"
                     /locus_tag="ACHKUN_001280"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO6659578.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(895..1563)
                     /gene="tcyL"
                     /locus_tag="ACHKUN_001281"
     CDS             complement(895..1563)
                     /gene="tcyL"
                     /locus_tag="ACHKUN_001281"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO6659579.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1578..2564)
                     /gene="dcyD"
                     /locus_tag="ACHKUN_001282"
     CDS             complement(1578..2564)
                     /gene="dcyD"
                     /locus_tag="ACHKUN_001282"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO6659580.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2669..3469)
                     /gene="tcyJ"
                     /locus_tag="ACHKUN_001283"
     CDS             complement(2669..3469)
                     /gene="tcyJ"
                     /locus_tag="ACHKUN_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6659581.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3557..4108)
                     /gene="fliZ"
                     /locus_tag="ACHKUN_001284"
     CDS             complement(3557..4108)
                     /gene="fliZ"
                     /locus_tag="ACHKUN_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO6659582.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4154..4873)
                     /gene="fliA"
                     /locus_tag="ACHKUN_001285"
     CDS             complement(4154..4873)
                     /gene="fliA"
                     /locus_tag="ACHKUN_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO6659583.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5038..6501)
                     /locus_tag="ACHKUN_001286"
     CDS             complement(5038..6501)
                     /locus_tag="ACHKUN_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO6659584.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6756..8168
                     /gene="fliD"
                     /locus_tag="ACHKUN_001287"
     CDS             6756..8168
                     /gene="fliD"
                     /locus_tag="ACHKUN_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6659585.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8183..8593
                     /gene="fliS"
                     /locus_tag="ACHKUN_001288"
     CDS             8183..8593
                     /gene="fliS"
                     /locus_tag="ACHKUN_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6659586.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8593..8958
                     /gene="fliT"
                     /locus_tag="ACHKUN_001289"
     CDS             8593..8958
                     /gene="fliT"
                     /locus_tag="ACHKUN_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO6659587.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9036..10523
                     /gene="amyA"
                     /locus_tag="ACHKUN_001290"
     CDS             9036..10523
                     /gene="amyA"
                     /locus_tag="ACHKUN_001290"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6659588.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10557..10970)
                     /gene="yedD"
                     /locus_tag="ACHKUN_001291"
     CDS             complement(10557..10970)
                     /gene="yedD"
                     /locus_tag="ACHKUN_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO6659589.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11157..12362
                     /gene="yedE"
                     /locus_tag="ACHKUN_001292"
     CDS             11157..12362
                     /gene="yedE"
                     /locus_tag="ACHKUN_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO6659590.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12359..12592
                     /gene="yedF"
                     /locus_tag="ACHKUN_001293"
     CDS             12359..12592
                     /gene="yedF"
                     /locus_tag="ACHKUN_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO6659591.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12699..13367
                     /gene="yedK"
                     /locus_tag="ACHKUN_001294"
     CDS             12699..13367
                     /gene="yedK"
                     /locus_tag="ACHKUN_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO6659592.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(13762..14076)
                     /gene="fliE"
                     /locus_tag="ACHKUN_001295"
     CDS             complement(13762..14076)
                     /gene="fliE"
                     /locus_tag="ACHKUN_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6659593.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14291..15949
                     /gene="fliF"
                     /locus_tag="ACHKUN_001296"
     CDS             14291..15949
                     /gene="fliF"
                     /locus_tag="ACHKUN_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6659594.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15942..16937
                     /gene="fliG"
                     /locus_tag="ACHKUN_001297"
     CDS             15942..16937
                     /gene="fliG"
                     /locus_tag="ACHKUN_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6659595.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16930..17616
                     /gene="fliH"
                     /locus_tag="ACHKUN_001298"
     CDS             16930..17616
                     /gene="fliH"
                     /locus_tag="ACHKUN_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6659596.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQMPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17616..18989
                     /gene="fliI"
                     /locus_tag="ACHKUN_001299"
     CDS             17616..18989
                     /gene="fliI"
                     /locus_tag="ACHKUN_001299"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6659597.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            19008..19451
                     /gene="fliJ"
                     /locus_tag="ACHKUN_001300"
     CDS             19008..19451
                     /gene="fliJ"
                     /locus_tag="ACHKUN_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6659598.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19448..20575
                     /gene="fliK"
                     /locus_tag="ACHKUN_001301"
     CDS             19448..20575
                     /gene="fliK"
                     /locus_tag="ACHKUN_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6659599.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            20680..21144
                     /gene="fliL"
                     /locus_tag="ACHKUN_001302"
     CDS             20680..21144
                     /gene="fliL"
                     /locus_tag="ACHKUN_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO6659600.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21149..22153
                     /gene="fliM"
                     /locus_tag="ACHKUN_001303"
     CDS             21149..22153
                     /gene="fliM"
                     /locus_tag="ACHKUN_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO6659601.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22150..22563
                     /gene="fliN"
                     /locus_tag="ACHKUN_001304"
     CDS             22150..22563
                     /gene="fliN"
                     /locus_tag="ACHKUN_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO6659602.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22566..22931
                     /gene="fliO"
                     /locus_tag="ACHKUN_001305"
     CDS             22566..22931
                     /gene="fliO"
                     /locus_tag="ACHKUN_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO6659603.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22931..23668
                     /gene="fliP"
                     /locus_tag="ACHKUN_001306"
     CDS             22931..23668
                     /gene="fliP"
                     /locus_tag="ACHKUN_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6659604.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23678..23947
                     /gene="fliQ"
                     /locus_tag="ACHKUN_001307"
     CDS             23678..23947
                     /gene="fliQ"
                     /locus_tag="ACHKUN_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO6659605.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23956..24741
                     /gene="fliR"
                     /locus_tag="ACHKUN_001308"
     CDS             23956..24741
                     /gene="fliR"
                     /locus_tag="ACHKUN_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6659606.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25031..25654
                     /gene="rcsA"
                     /locus_tag="ACHKUN_001309"
     CDS             25031..25654
                     /gene="rcsA"
                     /locus_tag="ACHKUN_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO6659607.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(25698..25886)
                     /gene="dsrB"
                     /locus_tag="ACHKUN_001310"
     CDS             complement(25698..25886)
                     /gene="dsrB"
                     /locus_tag="ACHKUN_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO6659608.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26049..26276
                     /gene="yodD"
                     /locus_tag="ACHKUN_001311"
     CDS             26049..26276
                     /gene="yodD"
                     /locus_tag="ACHKUN_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO6659609.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26574..27389
                     /gene="yedP"
                     /locus_tag="ACHKUN_001312"
     CDS             26574..27389
                     /gene="yedP"
                     /locus_tag="ACHKUN_001312"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO6659610.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(27386..29080)
                     /gene="dgcQ"
                     /locus_tag="ACHKUN_001313"
     CDS             complement(27386..29080)
                     /gene="dgcQ"
                     /locus_tag="ACHKUN_001313"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO6659611.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(29318..29500)
                     /gene="yodC"
                     /locus_tag="ACHKUN_001314"
     CDS             complement(29318..29500)
                     /gene="yodC"
                     /locus_tag="ACHKUN_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO6659612.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(29579..30496)
                     /gene="yedI"
                     /locus_tag="ACHKUN_001315"
     CDS             complement(29579..30496)
                     /gene="yedI"
                     /locus_tag="ACHKUN_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO6659613.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            30669..31589
                     /gene="yedA"
                     /locus_tag="ACHKUN_001316"
     CDS             30669..31589
                     /gene="yedA"
                     /locus_tag="ACHKUN_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO6659614.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(31578..32048)
                     /gene="vsr"
                     /locus_tag="ACHKUN_001317"
     CDS             complement(31578..32048)
                     /gene="vsr"
                     /locus_tag="ACHKUN_001317"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO6659615.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(32029..33447)
                     /gene="dcm"
                     /locus_tag="ACHKUN_001318"
     CDS             complement(32029..33447)
                     /gene="dcm"
                     /locus_tag="ACHKUN_001318"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO6659616.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33514..33951)
                     /locus_tag="ACHKUN_001319"
                     /pseudo
     CDS             complement(33514..33951)
                     /locus_tag="ACHKUN_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            34007..>34148
                     /locus_tag="ACHKUN_001320"
     CDS             34007..>34148
                     /locus_tag="ACHKUN_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO6659617.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTA"
BASE COUNT         8332 a         8861 c         8652 g         8303 t
ORIGIN      
        1 tattattcag gcaatgaatt acttttgcaa gccatcacat tctcttatgt tattaatgag
       61 ttatgctgat ttgttaagca gttttatcac gcttgaaata gcgtcccgcc ccgacaggtg
      121 aatcgtcggg gctgattttt tcttattatt gcagcagaaa cttctcgagg aactggcggg
      181 tgcgaggctg ctggggatcg gcaaataacg cttttgctgc cccctgctcg actatccgcc
      241 cctggtccat aaaaatcgcc cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca
      301 caatcaccat cgtgcgcttt tcctgcgcca gctgacggat ggtgttcagg acttcgccca
      361 ccagctctgg atccagcgcc gacgttggct cgtcaaacaa aatcacctca ggacgcattg
      421 ccagcgcacg tgcaatcgca acacgttgct gttgaccgcc agacaaacga cgtggatagc
      481 tggtttcttt accggccagc ccaactttcg ccaacagctc gcgagcgcgc gccgtggcct
      541 cttctttcgg ttcgcctttg acgatcaccg gcccttcaat aatgttttcc agcaccgtac
      601 gatgcggaaa caaattaaag ttctggaaga caaacccgac gtgctgacgc aactggcgaa
      661 tcagcgattt ttgctgactt aatgaacgtg cagtatcaat agtgatatcg ccaacggtga
      721 tcgttcccgc ttccggttgt tccagcagat ttatgctgcg tagcaacgtg gttttgccgg
      781 aaccactcgg accgataatt gccaccactt cgccaggctt tacctcgagg tcgataccgt
      841 gcagcaccgt ctgaccgtga aattttttca ccaagttctt aacttcaatg gcactcattt
      901 tggttctctc tcctggcgat taagttgatt ctcaaaatgg ttctgcaacg tcgataatac
      961 tgtcgccatg atccagtaga tgagcgaagc cgccaggtac atggtgaaca cctccagcgt
     1021 gcgcgaggta atcaactgcg cctgacggaa cagctccggc acctggattg tcgcggccag
     1081 cgacgtatcc tttaccaggc tgataaagct gttcgacagc ggcggcaacg ccacccgcgc
     1141 cgcctgtggc aaaatcgcgc gacgcatagt ctgccatggc gtcataccaa tactggcagc
     1201 tgcttcccac tgacctttat caatagaaga aatggcggcg cgcagtgttt cggcggcata
     1261 ggcggcagtg ttcagcgaca gaccaatcat tgctgacgga atgggatcta attcgatacc
     1321 aaactgcggc aggccgtaat agatcataaa cagttgtgcg atgagcggtg taccacgaaa
     1381 aatagagata taaaaacgcg ccagccagcg cacgggccag attggcgaca ggcgcatcag
     1441 cgcgagaata aacccgagca gtaagccaaa aaacatgccg ccgatactga gttgcagcgt
     1501 atacccagcc cctttcagca ggaacggcaa ggaatcaata accagttgta tactttcttg
     1561 catgagcgtt cccggagcta aacgtgggga tgataggcga acagcgcagg cgcaccgccg
     1621 gtatgaataa acagaatcgg cccttcatct ttgaagcgtt tctgactgat accgtcaatc
     1681 agccccgcca tcgcttttcc ggtatacaca ggatcaagca gaatgccttc cagccgcgcc
     1741 agcaatttca ctgcttccat gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag
     1801 tcatcccaga gtataatttc cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt
     1861 tgtaggttaa ccactttcgg caactgatcg gcaacggaac gcgacacggt cacgccaatc
     1921 agttcgcttt caggcatcag gtgttccagc ccaacagcca gtccggcgtg agttccggca
     1981 ctgcccgatg cgactaccac cgacgaaata ttaaccgccc cttcacactg ttgcgcgatt
     2041 tccagcgcac tctccacata acctagcgcg ccaagagcat tagaaccgcc aaccggaatg
     2101 acatacgggc gaaagccttg tgcttcgact cgcgtcgcca gctcttccag ttgggcattg
     2161 ggatcggtca gtgcttcgca catttcaatc tgggtattga acagatccag caacaagcga
     2221 ttgccgttgg ttaaatagtt ttctgcggtt gtgccaatag gattttccag cagcgccaca
     2281 cagtgcagac cgagtttcgc cgcaactgcg gcagtctgcc gcacatggtt agactggatc
     2341 gccccggcag taatcagcgt atcggcacct tcgcgcagag catctgctgc gagaaattcc
     2401 agcttacgta atttattgcc gcccattgcc atgggggtga cgtcatcccg tttgatgaaa
     2461 atttcccgtc caagataatc agaaaagcgc ggcagatatt cgagcggtgt tggcgcgccg
     2521 ataaactcca accgtggaaa acgggttaaa ttatgcagtg gcataacagc ctccgatgtg
     2581 tgttgttgtg attttcttat tatgcacgct gaaaacgcgt aaataaaaaa ggcgctggtg
     2641 aaagcgccct tttttgtcat tatgctgatt atttggtcac atcagcacca aaccattttt
     2701 cggaaagggc ttgcagagtg ccatcttttt gcatttccgc aattgcatcg ttcactgctt
     2761 tcagcaggtc ttcatttcct ttacgcagcg ccacgccaga ctcctgacgg gagaatgctt
     2821 caccggttac tgccagcgta tcgttggttt tcttcaccag atccagcgcc gccagacggt
     2881 caacgaggat cgcatcgata cggcctacgc gcagatcctg atatttggtc gggtcatcat
     2941 cataggtacg cacatcgacg ccctgaacat tctgccgcag ccactcttca tagttggtgc
     3001 ccagaccgac acccactttt ttgcctttca gatcagcagc cgttttaatg gtgccttcgt
     3061 tacctttttt caccagcgcc tgaataccag aaatggtgta cggggtagag aaatcgtatt
     3121 ttttcttgcg ctcatcagaa atggtgacct gattaatcac cacatcaatg cgtttagagt
     3181 ccagcgacgc cagcataccg tcccatttgg tcggttttag tgacgcctca acgccaagat
     3241 gttttgccag ctgttgggca aattccactt caaaaccggt taatttgccg tcatccccct
     3301 gaaaactgaa cggcggataa gtgccttcca gccctaccag cagcgtgccg cgctctttaa
     3361 ctttattaag cagaccttca tctgcaaaac ttttaacgct catgcccgca accagcgcta
     3421 cggccatcac actcatcaat gcctgacgtc ccagatgtgc taatttcata tttaccccga
     3481 atgttgttat gtctgtttgc agtgtagagc cattgtttgt aaacacaaaa acaactccgc
     3541 tacatcttat tcttatttaa tatatatcag aagcaggcag gctggaggat ttctgcacaa
     3601 gtccggtaca cgtttgacgc tgatagtgtt gatacttccg taatgcgatg cggtaattgt
     3661 tggtgctggt ggtgggcagc cacggggcga ggatttcatc aaggaaaccg tcctgcagcg
     3721 gatcgaggga aatattttgc tcgtgtagat gattccccaa acggcgcaaa cgaacgacat
     3781 attcacgcac cgtacctggg ctcatttcag tttgctcaaa aagaaattgc ttaaagccga
     3841 taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc accgcaaaat cggcacaatg
     3901 ccgcccatga tttttgttcc gtttgccagc cattttcatc aagcagcgcg tccaggcggg
     3961 aaatctcgat tttattcact attttgccgt cgcgaacgag ggtaatgcga tcgagtaatt
     4021 tacggcaatg cgcgcaatga gtctggctgt gtttaaagtc tttaagatag cggcttaatg
     4081 gccgtctttt caggtgctgc accatcatta agaactcctg gtagtcaaag ttaaagtgcg
     4141 gcatttactg acgttataac ttacccagtt tagtgcgtaa ccgtttaata gcctggctgt
     4201 gtaactgact gacccgcgat tccccgacct ccagcaccgc gccaatctct ttgagattca
     4261 gctcttcctg gtaatagagg gttaatacca gtttttcgcg ctccggcaac gtttcgatgg
     4321 cttccatcac ccgctgacgc agattactgt ccagcagttg ttgtagcggg ttttctcgct
     4381 gatgatcatc agtaaccagt tcgatgctat cgccgtgctc ttcgcgccac tcatcgtagg
     4441 agaagagctg gctgttattg gtgtcgagca acatttggcg ataatcggca atatcgatcc
     4501 ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc aagttcctgc tccagttgcc
     4561 ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct gcgcggcacc cagtcacggc
     4621 tgcgaagttc atccagcata gcgccacgga tacgctgcac tgcgtaagtt gtaaatgccg
     4681 ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa cccaatgccg cccgcctgta
     4741 gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg caggcgcaat gcttcgtgac
     4801 gcaccagcgg gacataacgc tgccatagcg agtgtttatc cattacacct tcagcggtat
     4861 agagtgaatt cacgataaac agccctgcgt tatatgagtt atcggcatga ttatccgttt
     4921 ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag gggttatttg ggggttaccg
     4981 gtaaattgcg ggcagaaaaa accccgccgg tggcgggggt tgagcgataa gtgtaaatta
     5041 acgcagcaga gacagtacgt tctgcgtggt ctggttagcc tgcgccagaa cagaggtacc
     5101 cgcttgttgc aggatctgcg cacgagacat gttagacact tcggtcgcgt agtcagcatc
     5161 ttcgatacgg ctacgggcag aagacaggtt gtttacggtg ttgccaaggt tggtgatagc
     5221 agagtcgaaa cggttttgta ctgcaccgag gtcagaacgc agattgtcaa ctttagccaa
     5281 tgctttgtcg atagtttcga gcgggttggt ggtagattgc aacgattttg ctgcatcttc
     5341 gtttaccaga atcgggctac caccttctga tttgctcaga tacatgactt tattgttacc
     5401 agaagcagtc tccgttatcg ttttaccatc tgcactaaca tcgtaagttg caccgttaac
     5461 aactaacgtg cttcctgttt tcttagctgc attgagatca agatcggaaa gtgtggcagc
     5521 cttgttatca actttggttg tcgtataaag gccagcgcta tttttgtaaa ctgtctcact
     5581 cgtaatcaaa gaagcttttt tgtcagcatc tgtcacagta aatgtcactg acttaccatc
     5641 aatttctgca gaatatacac cattcccatt gccatccgct gcagttgttg ttgtgaattc
     5701 aactccatta aacgtgaatt tatcattctg tgctttattc ttagccgctg ctgcgagtgc
     5761 tgtagcatca atccctgtac cagctatgtt tgtgtcagat ttagttgtca gtgaaccatc
     5821 tgctgcacta acaaaaatat cattccctgt agtatcttta acagctccgc tatctacgtt
     5881 aacagtataa gcatcaccgc caacatcata gttatcagta cctgtcgctt taaatttaga
     5941 aatcaggtca ctgccagttg ctttctgcgc gccatcgata ttaaaaccgt ccaggccgag
     6001 agttttcgca tcaatttttg ccagattgat agtgatggtt tcaccatcat tagcaccaac
     6061 ctgaattttc atttcattat tttcagcaag gactttcacg ccgttaaact gagtttgctc
     6121 agatacacgg tcaatttctt ccagacgttg agtaatttca gcctggatag aagaaagatc
     6181 gctgtcagag ttagtaccgt tagttgcctg aacagtcagt tcacgtacac gctgcaggtt
     6241 gttgttaatt tcattcagcg caccttcagt ggtctgcgca acagaaatac catcattcgc
     6301 gttacgggaa gcctgagtca gacctttaat atttgctgta aaacggttag caatcgcctg
     6361 acctgctgcg tcatctttag cactgttaat acgcaggcca gaagagagac gttcaatggc
     6421 ggagctcaga gaagactgag atttgttcag gttattctgg gtcaacagcg acaggctgtt
     6481 tgtattaatg acttgtgcca tgatcttttc cttatcaatt acaacttgat gttattgggc
     6541 tgttgcccac ggtttctcac cgtacccctt gtatcggcac ctgaatttcg aactttagaa
     6601 aattttagtt tctctcctgt tttttcttaa cggctgaaat atccgtaata tacaactcta
     6661 ctctgcgcat catcttcgtg aatcaactat taaactttgc gcacaaattg ccgataaccc
     6721 gcttatctac tgtttgcaat caaaaggaat taggtatggc ttcatttaca tctcttggcg
     6781 ttggttcgaa tctgccgctg gatacattgc taaacaacct gaccatcgct gaaaaaaaac
     6841 gcttaaaccc aattactcag caacagagtg acaataccgc acgtctgaca gcctatggca
     6901 cattaaaaag tgcattagaa aaattccaga ctgcaaatac tgcgttaaat aaagccgatc
     6961 tgttcaaaag cactaccgtt accagtagca atgaagacct taaagtttcc accgaagcag
     7021 gtgctgcccc cggtatctat accatcagcg taacccaact ggcgcaggcg caatctttac
     7081 gaactgattc cccgacaatc attgcatcaa caaaagatgc actgggtgat gagtcatcag
     7141 atacacgcac aataaaaatt acccaagacg gtcgtaaaga gccgctggaa atcaagctca
     7201 ataaagatca gacgtcattg gatgagatca gcaaagccat taacgatgcc gatagtggaa
     7261 tttccgctag tatcgttaaa gtcaaagatg gtaactatca attggtattg accgccagtg
     7321 aaggtctggc caataaaatg accatatctg ttgagggtga cagtaaatta aatgatctgc
     7381 tggcttatga cagcaagacc aacaccggga atatgaagga actggttaac gcgcaaaatg
     7441 cacagctaaa cgttaacggt atcgatattg aacgttcaag taacaaaatt accgatgctc
     7501 ctcagggagt gactctcgat ttgaccaaaa aagtcactga cgtccgggtc acggtgacaa
     7561 aaagcaacga taaagcaacc gaagcaatta aaggctgggt ggattcatat aactccctca
     7621 ttgacacctt taacaccctg acaaaatata aagaagtaga cccaggtgct gaagcacaag
     7681 ataaaaataa tggtgcactt ctcggagaca gcgttgtacg tacaattcaa agcggcatcc
     7741 gcgcacaatt tgcaaacggt gccagcgaca gtgccttcaa aacattaaac gagatcggga
     7801 ttaagcagga cggcacgacg ggtaagctga aaattgatga tgataaactc aaaaaagtcc
     7861 tgaatgaaaa tacagcatcc gtacgtgaat tgctggtagg cgatggtaaa gaaacaggaa
     7921 ttaccaccaa aattgccacc gaggtaaaag gttaccttgc cgatgatggc attattgata
     7981 gcgcacagga cagtattaac gccacactga aaaaactgac caaacagtat ctatccgtca
     8041 gtgccagcat tgatgacacc gtggcgcgtt acactgcaca gtttacgcaa ttagatacca
     8101 tgatgagcaa actgaataac accagtacct atctcagcca acaatttact gcaatgagta
     8161 attcctgatc acggaggtca gcatgtatac cgcgagcggc tcaaaagcct atgcacaaat
     8221 tggcgtcgaa agcgccgtaa tgagcgccag ccagcagcag ctggtcacca tgctatttga
     8281 tggagtgctg agcgcactgg ttagagcacg cctgtttatg caggacaaca atcagcaagg
     8341 caaaggcgtc tctttgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct
     8401 tgatgaagag agcaaagacg aattaaccca aaacttgatt gctctttata gctatatggt
     8461 caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aagtggaagc
     8521 attaatgcgc aatattgccg atgcctggaa agagtcgtta ctctcccctt ctttgattca
     8581 ggacccagtc tgatgaacca cgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa
     8641 aaaagccagc tcatgttacg cctggcaaca gaagaacaat gggacgaact catcgccagc
     8701 gagatggcgt atgtgaatgc ggtgcaggag attgcacatc tgactgaaga gattgacccg
     8761 tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga caacgaaagc
     8821 aaggtaaagc agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca
     8881 tcggtgcaaa aatcggtgtt aagtgcctat ggtgatcagg gcggctttgt gctggctccg
     8941 caggataacc tcttttgaat ctgaacgagt cgatggctcg cgaataatcc gattacggct
     9001 acgcttctaa tgttcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat
     9061 gttttcactg gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgccg
     9121 acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat
     9181 cgggcgggta ttcggtcggc tacgactcct atgatttatt tgatttaggc gagtttgatc
     9241 agaaaggcag catccctact aaatatggcg ataaagcaca actgctggcc gccattgacg
     9301 ccctgaaacg taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg
     9361 cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc tgatgaccgt acgcaaattg
     9421 acgaagaaat cattgagtgt gaaggctgga cgcgttacac cttccccgcc cgtgccgggc
     9481 aatactcgca gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaacc
     9541 ctgatgaaga tggcatattt aaaattgtta acgactacac cggcgaaggc tggaacgatc
     9601 aggttgatga tgaattaggt aatttcgatt atctgatggg cgagaatatc gattttcgca
     9661 atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat
     9721 gcgacggttt tcgtcttgat gcggtcaaac atattccggc gtggttttat aaagagtgga
     9781 tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggcagag tactggtcgc
     9841 atgaagtcga taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgttcg
     9901 atgcgccgct gcagatgaaa ttccatgaag catcgcgcat ggggcgcgac tacgacatga
     9961 cgcagatttt cacgggtaca ttagtggaag ccgatccttt ccacgccgtg acgctcgttg
    10021 ccaatcacga cacccaacca ttgcaagccc tcgaagcacc ggtcgaaccg tggtttaaac
    10081 cgctggcgta tgctctaatt ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg
    10141 acctctacgg tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata
    10201 tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg
    10261 tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt ggcactgacg
    10321 aatatcccgg ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa accattcatc
    10381 tgggagagaa ttacggcaat aaaacctggc gtgattttct ggggaatcgg caagagagcg
    10441 tagtgaccga cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt
    10501 gggttatcga agaggtgatt taaattcatc cccggcggca agccggggag atttcattac
    10561 ggcagtggcg tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga
    10621 tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg
    10681 tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt caacttgccc
    10741 ggcacggcaa tcacccgctg ccactgacgg caatccaggg tatcgccctc tttagtgaca
    10801 atcaggctgc ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag
    10861 tagcccgcta gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg
    10921 gcacagcccg ccagcaacat gagcgcgctt gcaattgcga gttttttcat cattcgtcct
    10981 gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggtt
    11041 cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc
    11101 atgatgtatg attcgcgatt cttgtttcaa accttctgag ttcagaggct acattcatgt
    11161 catggcagca attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca
    11221 tcgcggcggg tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca
    11281 cgggtgaatt tacccgttgg ggcggccagc tcctgcagct gtttggcgtc catgctgaag
    11341 agtggggtta ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggga
    11401 tgatgatcct cggcatgttt ggcggctgct ttgccgcagc gctgtgggcc aacaatgtca
    11461 aactgcgcat gccacgcagc cgtatccgca ttatgcaggc cattattggc ggtattatcg
    11521 ctggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggtattc
    11581 ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg
    11641 gcgcacgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg
    11701 ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc
    11761 tggtcttttt cggtatgctg ggctgggcgc tgctcacggc gatgaaccaa ccaaaactgg
    11821 ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct
    11881 ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca
    11941 ttatcggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac
    12001 ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg tttactgttt ggttttggca
    12061 tcgtgctggc aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc
    12121 actactggtg ggtcggtctg ggcaatgtga tcggctcgac gattctggcg tattactggg
    12181 atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg
    12241 gcccgatggg tggcctgctg gtgacatatt tgctgttgtt tactgcgctg atgttgatta
    12301 tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gactgctaag gagatcgcat
    12361 gaaaaatatc gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc
    12421 agtcgcaact cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg aagtggtgag
    12481 cgactgtcca cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt
    12541 gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat
    12601 tcccgctgtg gcccacagcg ggaacatcgt tgcctctgac acatagattt tacccgcccc
    12661 ctctcctatt cttttcagta tgatctcagg aggtttctat gtgtggacgc tttgcccaat
    12721 cccaaacgcg tgaagattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct
    12781 acgatcccga acccattggc agatacaacg tcgcgccggg aaccaaagtt cttctgctca
    12841 gtgaacgtga tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt
    12901 gggataaacc gccgctgatt aacgcccgcg tagaaactgc ggccaccagt cgtatgttta
    12961 aaccgctctg gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa
    13021 aagaaggcga caaaaaacag ccttatttta tctatcgcgc tgatggacaa cctgttttta
    13081 tggccgcgat aggtagcaca ccgtttgagc gcggtgacga agccgaagga tttttaattg
    13141 tcactgcagc agcagaccag ggtctggtag atattcatga ccgccgcccg ctggttctgt
    13201 cgccagaagc tgcacaggaa tggatgcggc aagagattgg cggtaaagaa gcatcagaaa
    13261 ttgctaccaa cggctgtgtg ccagcaaacc agttcacctg gcacccggta tcgcgcgcgg
    13321 tgggtaatat caaaaaccag ggagcggagt taattcaacc ggtttgataa gccaggagac
    13381 tattgatcaa taatactgcg tcataagaaa tctctattag acaatagtag acgctccttc
    13441 cacatgcgcc acaagaaacc tctattccag tgacacaatt acgcctgatt atttccctag
    13501 cccggaatat gaataaaaac atcaacaaag attaaattat tcaaacgtta ctgttgcgtc
    13561 tgcggtcgta gcaacatccg acgtggtgat gagtgttaaa agagacactc tgatgttcag
    13621 agggattatc gcgtttacgg atcgtggatg gcagaaaact ttcaggatca ggtatccatt
    13681 ttaaaccaga aattgagtga gtttgcccca tccatgcccc aagcggtgag attggaagta
    13741 attaacaaca ggttacaaaa cctacacctg catggacatc acttcctgat acgccgccac
    13801 cagcttatta cgcacctgaa tccccatttg catagaaact gaggcttttt gcatatcggt
    13861 catcacatcg tttaacgcca cgccgggttc accgagggtg aatttttctg cctgcgtgcg
    13921 ggcagctgtt tgtgtatcgc taatgcgatc gagcgtggcg tgcagctgcc cggcaaaact
    13981 aatggtcggt tgcggcagtg aatcctgcgc acgcgcactc atcgccgtag cctgtaactg
    14041 gctgataacc ccttcaatcc cctgtatcgc tgacattttc gtctcctgga taatttctgg
    14101 tagcaaagcc taccagtaag tcaataagat aaaggcgcta aataacaaca aaaaaacggg
    14161 tttattggcg gatagaaaaa aacgaaagca caaataatgg cagcgtcaat ttttcgagtt
    14221 tgctgacccg ggagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac
    14281 gaggtgcgcg atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct
    14341 gcgtgcgaat ccgaaaattc cattgattgt tgccggttcc gcggcagtgg cggtcatggt
    14401 cgcactgatc ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga
    14461 tcaggatggt ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag
    14521 cgaagccagc ggcgctattg aagttccggc agataaagtt cacgaactgc gtctgcgcct
    14581 ggcacaacaa ggtttgccca aaggcggcgc ggtcggtttc gaactactcg atcaggaaaa
    14641 gtttggtatc agccagttca gcgaacaggt gaattatcag cgggcgctgg aaggcgagct
    14701 ttctcgtacc atcgaaacta tcggcccggt aaaaggggcg cgcgtacatc tggcaatgcc
    14761 gaaaccgtct ttattcgtcc gtgaacaaaa atccccttct gcatcggtga cggtaaatct
    14821 gttacccggc cgcgcactcg atgaagggca aattagcgcc attgtgcatc tggtttccag
    14881 cgccgttgct ggtctgccgc cgggaaacgt cacgctggtg gatcagggcg gacatctgtt
    14941 aacccagtcc aataccagcg ggcgcgatct taatgatgct cagttgaaat atgccagcga
    15001 tgtcgaaggc cgtattcagc ggcgtattga agcaatcctg tcgcctattg ttggtaacgg
    15061 taatattcac gcccaggtca cggcgcagct ggacttcgcc agtaaagaac aaacggaaga
    15121 acagtatcgc cctaacggtg atgaatctca tgcggcgctt cgttcacgcc agcttaatga
    15181 gagcgagcaa agcggttccg gttatccggg cggcgtaccg ggggcgttgt cgaatcaacc
    15241 ggcacctgcg aataacgcgc caatcagcac gcctccggca aatcaaaata accgccagca
    15301 gcaggcgagc accaccagca atagcgggcc gcgtagcaca cagcggaatg aaaccagtaa
    15361 ctacgaagtc gatcgcacca ttcgtcatac caaaatgaac gtgggcgatg tgcaacgtct
    15421 gtcagtcgcg gtagtggtga attacaaaac cttgccagac ggtaaaccat tgcctctcag
    15481 caacgaacag atgaagcaaa ttgaagatct gacccgcgag gcgatgggct tttctgaaaa
    15541 acgcggtgac tcgctcaatg tcgttaactc gccgttcaat agcagtgacg aaagcggcgg
    15601 agaactgccg ttctggcaac agcaagcgtt tatcgatcag ttgctggctg ccggtcgctg
    15661 gttgctggta ctgctggtgg cgtggctgct gtggcggaaa gcggtacgcc cgcagttaac
    15721 acgtcgcgcc gaggcgatga aagctgtaca gcaacaggcg caggcccgtg aggaagtgga
    15781 agatgcggtg gaagtccgcc tgagcaaaga cgaacaactc caacaacgac gcgctaacca
    15841 acgtctgggg gcagaagtca tgagccagcg tatccgtgaa atgtctgata acgatccgcg
    15901 cgtggtggcg ctggtcattc gccagtggat aaataacgat catgagtaac ctgacaggca
    15961 ccgataaaag cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca gaggtgttca
    16021 agcacctctc ccagcgcgaa gtgcaaaccc tgagcgctgc aatggcgaac gtcacgcaga
    16081 tctccaacaa gcagctaacc gatgtgctgg cggagtttga gcaagaagct gaacagtttg
    16141 ccgcactgaa tatcaacgcc aacgattatc tgcgttcggt attggtcaaa gctctgggtg
    16201 aggaacgtgc cgccagcctg ctggaagata ttctcgaaac tcgcgatacc gccagcggta
    16261 ttgaaacgct caactttatg gagccgcaga gtgccgccga tctgattcgc gatgagcatc
    16321 cgcaaattat cgccaccatt ctggtgcatc tgaagcgcgc ccaggccgcc gatattctgg
    16381 cgttgttcga tgaacgtctg cgccacgacg tgatgttgcg tatcgccacc tttggcggcg
    16441 tgcagccagc cgcgctggcg gaactgaccg aagtactgaa tggcttgctc gacggtcaga
    16501 atctcaagcg cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc aacctgatga
    16561 aaactcagca ggaagaagcc gttattaccg ccgtgcgcga attcgacggc gagctggcgc
    16621 aaaaaattat cgacgagatg ttcctgttcg aaaatctggt ggatgtcgac gatcgcagca
    16681 ttcagcgtct gttgcaggaa gtggattccg aatcgctgtt gatcgcactg aaaggggccg
    16741 agcagccact gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc gatattctgc
    16801 gcgacgatct cgccaaccgt ggtccggtgc gtctgtcgca ggtggaaaac gaacagaaag
    16861 cgattctgct gattgtgcgc cgccttgccg aaactggcga gatggtaatt ggcagcggcg
    16921 aggataccta tgtctgataa tctgccgtgg aaaacctgga cgccggacga tctcgcgcca
    16981 ccacaggcag agtttgtgcc catggtcgag ccggaagaaa ccatcattga agaggccgaa
    17041 cccagccttg agcagcaact ggcgcaactg caaatgcagg cccatgagca aggttatcag
    17101 gcgggtattg ccgaaggtcg ccagcaaggt cataagcagg gctatcagga aggactggcc
    17161 caggggctgg agcaaggtct ggcagaggcg aagtctcaac aagcgccaat tcatgcccgg
    17221 atgcagcaac tggtcagcga atttcaaact acccttgatg cacttgatag tgtgattgct
    17281 tcgcgcctga tgcagatggc gctggaggcg gcacgtcagg tcatcggtca gatgccaaca
    17341 gtggataact cggcactgat caaacagatc caacagttgt tgcagcaaga accgttattc
    17401 agcggcaaac cgcaactacg cgtgcacccc gatgatctgc aacgtgtgga tgatatgctc
    17461 ggcgcaacct taagtttgca tggctggcgc ttgcggggcg atcccacact ccatcctggc
    17521 ggctgtaaag tctccgccga tgaaggcgat ctcgacgcca gtgtcgccac tcgctggcaa
    17581 gaactctgcc gtctggcagc accaggagtg gtgtaatgac cacgcgcctg actcgctggc
    17641 taaccacgct ggataacttt gaagccaaaa tggcgcagtt gcctgcggta cgtcgctacg
    17701 ggcgattaac tcgcgctacc gggctggtgc tggaagccac cggattacaa ttgccgctcg
    17761 gcgcaacctg tgtcattgag cgccagaacg gcagcgaaac gcacgaagta gaaagcgaag
    17821 tcgttggctt taacggtcaa cggctgtttt taatgccgct ggaggaagtc gaaggtgtcc
    17881 tgcccggcgc gcgtgtttat gccaaaaaca tttcggcaga agggctgcaa agcggcaagc
    17941 agttgccgct cggtccggcg ttattaggtc gtgttctgga cggcagcggt aaaccgctcg
    18001 atggcctccc ctcccccgat acgacggaaa ccggtgcgct gattacccca ccatttaacc
    18061 cgttgcaacg tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca atcaatgccc
    18121 tgcttaccgt tgggcgtggg cagcgtatgg ggctgtttgc cgggtccggc gttggtaaaa
    18181 gtgtgctgct ggggatgatg gcacgttaca cccgcgccga tgtcattgtc gtgggtttga
    18241 ttggtgaacg cggacgcgaa gtaaaagatt ttattgagaa tatcctcggt gccgaagggc
    18301 gtgcacgctc cgtggtgatt gccgctccgg cggatgtctc tccgcttctg cgaatgcaag
    18361 gtgccgccta tgccacgcga attgccgaag attttcgcga tcgtggtcag catgtgttgc
    18421 tgattatgga ctccctcacc cgctacgcga tggcccagcg tgaaattgcg ctggcgattg
    18481 gcgaaccacc tgccactaaa ggttatccac cgtcggtgtt tgccaaatta ccggcactgg
    18541 tcgagcgtgc cggaaatgga attagcggcg gcggctcgat taccgcgttt tataccgtgc
    18601 tcaccgaagg cgatgaccag caggacccca ttgccgactc tgcgcgggca atcctcgacg
    18661 gtcacattgt gctgtctcgc cgactggcgg aagccgggca ctatccggct atcgatattg
    18721 aagcgtcgat cagccgcgca atgacggcgt tgatcagtga gcaacattac gcgcgagtgc
    18781 gcaccttcaa acagctgttg tcgagttttc agcgtaaccg cgatctggtt agcgtcggcg
    18841 cgtatgccaa aggcagcgat ccgatgctcg acaaagccat cgccctgtgg ccgcagctgg
    18901 agggctattt gcaacaaggc atttttgaac gcgcggactg ggaagcgtct ctccaggggc
    18961 tggagcgtat tttcccgaca gtgtcataac ccaggagata acggcagatg gcagaacatg
    19021 gcgcgctggc gaccctgaaa gatctggcag aaaaagaggt agaggatgcc gcgcgcctgc
    19081 tgggtgaaat gcgtcgcggg tgtcagcagg cggaagaaca gctcaaaatg ctgatcgatt
    19141 atcagaatga atatcgcaat aacctcaaca gcgatatgag tgccgggatg accagcaacc
    19201 gctggatcaa ctatcagcag tttatccaga cgctggaaaa agccattact cagcatcgcc
    19261 agcaacttaa tcagtggacg cagaaagttg acattgccct gaacagctgg cgagaaaaga
    19321 aacaacgttt gcaggcctgg cagacactgc aggaacggca atccacggcg gcactgcttg
    19381 cagaaaaccg cctcgatcag aaaaagatgg atgagttcgc ccagcgcgcc gccatgagga
    19441 aacctgaatg attcgcttag cgcccttaat taccgccgac gttgataacg ccacattgcc
    19501 tggcggcaaa gccagcgatg ctgcacaaga ttttctcgcg ttgttgagcg aagcattagc
    19561 aggcgagaca actaccgaca aagcggcccc ccagttgctg gtggcaacag ataagcccac
    19621 gacaaaaggc gagccgctgg tcagcgagat tcttgccgat gcacaacaag cggatttact
    19681 gatccctgtg gatgaaacac cgcctgtcat caacgacgaa caatccacat caacaccatt
    19741 aaccaccgct caaacgatga cgttggctgc ggtggctgac aacaatacgg caaaagacga
    19801 aaaagcggat gatctgaatg aagacgtcac cgcaagcctg agcgcccttt ttgcgatgtt
    19861 gccgggtttt gacaatacgc ccaaagtgac tgatgtgccg tcaaccgtgt taccggcaga
    19921 gaaaccaacg ctcttcacaa aactgacttc tgcgcaactc acaacagcac agcctgatga
    19981 tgcccccggc acgccagctc agccattaac accgctggta gcagaagccc agagtaaagc
    20041 ggaagtcatc agcacacctt caccggtgac cgctgccgcc agcccgctaa tcactccaca
    20101 ccagacacag ccactgccca ccgtcgccgt gcctgtgttg agtgcaccgc tgggttctca
    20161 cgaatggcaa caatcattaa gccagcatat ttcgctgttc acccgccaga ggcaacaaag
    20221 tgcagagttg cgtctgcacc cgcaggattt aggtgaagtg caaatctccc tcaaagtgga
    20281 tgataaccag gcacaaatcc agatggtttc accgcatcag catgtacgcg ccgcgctgga
    20341 agcagcgctt ccggtactgc gtacgcagct ggccgaaagt ggcattcagt tagggcaaag
    20401 caacatcagt ggcgaaagct ttagtggtca gcagcaggcc gcttcccaac aacagcaaag
    20461 ccaacgcaca gtaaaccatg aacctctggc gggggaagac gacgatacgc ttctggttcc
    20521 cgtctctata caagggcgcg taacaggcaa cagcggcgtt gatattttcg cctaacgtca
    20581 gaggtagcac cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat
    20641 tagccgataa gcagtagcga cacaggaaga ccgcaacaca tgactgatta cgcgataagc
    20701 aagaaaagca agcgatcgct ttggatcccg attctggtat tcattaccct cgcggcctgt
    20761 gccagcgcag gttacagcta ctggcattcg catcaggttg ccgctgacga caaagcgcag
    20821 caacgcgtcg taccctcccc ggtcttctac gcgctggata ccttcacggt caatctgggc
    20881 gatgcggatc gcgtgcttta tatcggcata accctgcgcc tgaaagatga agctacccgc
    20941 tcgcggctga gtgagtattt gccggaagtc cgtagccgtt tgctgttact gttttcgcgt
    21001 caggatgctg ccgtactggc gacagaagaa ggcaagaaaa acctgattgc cgagattaaa
    21061 accacgcttt ccaccccgct tgttgccggg caaccgaaac aggatgtcac cgacgtgctg
    21121 tataccgctt ttattctgcg ataacgacat gggcgatagt attctttctc aagctgaaat
    21181 tgatgcgctg ttgaatggtg acagcgaagt caaagacgaa ccgacagcca gtgttagcgg
    21241 cgaaagtgac attcgtccgt acgatccgaa tacccaacga cgggttgtgc gcgaacgttt
    21301 gcaggcgctg gaaatcatta atgagcgctt tgcccgccat tttcgtatgg ggctgttcaa
    21361 cctgctgcgt cgtagcccgg atataaccgt cggggccatc cgcattcagc cgtaccatga
    21421 atttgcccgc aacctgccgg tgccgaccaa cctgaacctt atccatctga aaccgctgcg
    21481 cggcacaggg ctggtggtgt tttcaccgag tctggtgttt atcgccgtgg ataacctgtt
    21541 tggcggcgat ggacgcttcc cgaccaaagt ggaaggtcgc gagtttaccc ataccgaaca
    21601 gcgcgtcatc aaccgcatgt taaagctggc gcttgaaggc tatagcgacg cctggaaggc
    21661 gattaatccg ctggaagttg agtacgtgcg ttcggaaatg caggtgaaat ttaccaatat
    21721 caccacctcg ccgaacgaca ttgtggttaa cacgccgttc catgtggaga ttggcaacct
    21781 gaccggcgaa tttaatatct gcctgccttt cagcatgatc gagccgctac gggaattgtt
    21841 ggttaacccg ccgctggaaa actcgcgtaa tgaagatcag aactggcgcg ataacctggt
    21901 gcgccaggtg cagcattcac agctggagct ggtcgccaac tttgccgata tctcgctacg
    21961 cctgtcgcag attttaaaac tgaaacccgg tgacgtcctg ccgatagaaa aacccgaccg
    22021 catcatcgcc catgttgacg gcgtcccggt gctgaccagt cagtacggca ctctcaacgg
    22081 tcagtatgcg ttacggatag aacatttgat taacccgatt ttaaattctc tgaacgagga
    22141 acagcccaaa tgagtgacat gaataatccg gccgatgaca acaacggcgc aatggacgat
    22201 ctgtgggctg aagctttgag cgaacaaaaa tcaaccagca gcaaaagcgc tgccgacgcg
    22261 gtgttccagc aatttggcgg tggtgatgtc agcggaacgt tgcaggatat cgacctgatt
    22321 atggatattc cggtcaagct gaccgtcgag ctgggccgta cgcggatgac catcaaagag
    22381 ctgttgcgtc tgacgcaagg gtccgtcgtg gcgctggacg gtctggcggg cgaaccactg
    22441 gatattctga tcaacggtta tttaattgcc cagggcgaag tggtggtcgt tgccgataaa
    22501 tatggcgtgc ggatcaccga tatcattact ccgtctgagc gaatgcgccg cctgagccgt
    22561 tagtgatgaa taaccacgct actgtgcaat cttccgcgcc ggtttctgct gcgccactgc
    22621 tgcaggtgag cggcgcactc atcgccatta ttgccctgat cctcgctgct gcctggctgg
    22681 taaaacggtt gggatttgtc cctaaacgca ctggcgttaa cggtctgaaa attagcgcca
    22741 gtgcttcgct gggcgcgcgt gaacgggttg tggtggtcga tgtggaagat gcacggctgg
    22801 tgctcggcgt taccgcaggg caaatcaatc tgctgcataa acttccccct tcagcaccaa
    22861 cggaagagat accgcagccc gattttcagt cggttatgaa aaacttgctt aagcgtagcg
    22921 ggagatcctg atgcgtcgtt tgttgtctgt cgcacctgtc cttctctggc tggttacgcc
    22981 cctcgccttc gcgcaactgc cgggtatcac cagccagccg ctgcctggcg gtggacaaag
    23041 ctggtcgctc ccggtgcaaa cgctggtgtt catcacctcg ttgacgttta ttccggcaat
    23101 tttactgatg atgaccagtt tcacccgcat catcattgtt tttggtttat tgcgtaacgc
    23161 gttgggaaca ccctccgcgc cacctaacca ggtattgctg ggactggcgc tgtttttgac
    23221 cttttttatt atgtcgccgg tgatcgataa aatttatgtc gatgcgtacc agccattcag
    23281 cgaagagaaa atctcaatgc aggaggcgct ggaaaaaggg gctcagccgc tgcgtgagtt
    23341 tatgctgcgt cagacccgcg aggcggattt agggttgttt gccagactgg cgaatactgg
    23401 cccgttgcag ggacctgaag ctgtgccgat gcgcattttg ctcccggcct acgtgaccag
    23461 cgagttgaaa accgcatttc agataggctt cacgattttc attccttttt tgattatcga
    23521 cctggtgatt gccagcgtgt tgatggcatt ggggatgatg atggttcccc cagccacaat
    23581 tgctctgccc tttaaactga tgctgtttgt actggtggat ggctggcaat tgctggtcgg
    23641 ttcgctggcg cagagctttt acagctagag aggcaaaatg acacctgaat cggtcatgat
    23701 gatggggact gaagcgatga aagtcgcgct ggcactggct gccccactgt tgctggtggc
    23761 gctggtcacg ggtcttatca tcagtatttt gcaggccgcc acccagatta acgaaatgac
    23821 gctgtcgttt attccgaaaa tcatcgccgt atttatcgcc attattattg ccggaccgtg
    23881 gatgctcaat ctgttgctgg attacgtccg caccttgttc actaacctgc cgtatatcat
    23941 cgggtaggcc gtacaatgat gcaggtaaca agcgatcaat ggctttcctg gttaagcctg
    24001 tacttctggc cgttactgcg cgtgctggcg ctcatttcta ccgcaccaat tctgagcgaa
    24061 cgcagcgtgc cgaaacgggt aaaactgggt ctggcaatga tgatcacgtt cgctattgcc
    24121 ccatcattac ctgccaacga tgttcctgtt ttttcgttct ttgctctgtg gctggccgtg
    24181 cagcagatcc tgatcggcat tgcgcttggt tttaccatgc aatttgcctt tgccgctgtg
    24241 cgaaccgctg gtgaaattat cggtctgcaa atggggctgt catttgcgac gtttgtcgat
    24301 ccggccagcc atctcaatat gcccgtttta gcgcgtatca tggatatgct ggcgttactg
    24361 ctgttcctga catttaacgg tcatttatgg ttgatttcac tgctggccga tacctttcac
    24421 accctgccaa ttggtggcga accgttgaac agcaatgcgt ttctggcact caccaaagca
    24481 gggagtttga ttttccttaa cgggctgatg ctggcattac cgctcattac tctgctgctg
    24541 accctgaatc tggcattagg cttacttaat cgtatggccc cgcaattatc catttttgtt
    24601 attggatttc cattaattct gactgtcggt atctctttaa tggcggcatt aatgccgtta
    24661 attgcgcctt tttgcgaaca tttattcagt gagatgttta atttgctggc tgatattatt
    24721 agtgaattgc cattaatata attccgtaac gtttatcatg ttatcctaag gattatccga
    24781 aaaataatac ctacgaacat cttccaggat actcctgcgg cgaaatattt gttttaagct
    24841 cactcacata tcgcaacatt tactttactt taagacaatt ccaggcaaat tatacaacac
    24901 tttacgggat agtaagtccg cctgaaaaat cgcgagagtg gcgcattagg tgacccatgt
    24961 tgttccgttt agtcatgatg aaatattcag gtaaggggaa ttatcgttac gcattgagtg
    25021 agggtatgcc atgtcaacga ttattatgga tttatgtagt tacacccgac taggtttaac
    25081 cgggtatctg ttgagtagag gggttaaaaa aagagaaatc aacgacattg aaaccgttga
    25141 tgaccttgcc atagcttgtg attcacagcg cccttcagtg gtgtttatta atgaggactg
    25201 tttcatccac gatgcttcta acagtcagcg tatcaagctc atcattaatc aacatcccaa
    25261 tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgaat atctattggt
    25321 cagaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg acgatatcct
    25381 tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc cgacgttatc
    25441 attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca
    25501 aatctctgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag gtaatattaa
    25561 acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac tgacggataa
    25621 tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg gtttcccacc
    25681 agtcaggccg aattagatta ctctgctttc tccacaaaga taccgtcctg atgccctgct
    25741 tcattaaaga accagatgcc gagcgggtag tcttccagcg aaaccaggta cattgtgcct
    25801 tcactaaact cctcaactgc cagtaccacg ccaggacgac gcggaccgcc atccgttttg
    25861 actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa
    25921 caatttcgtt tttctggcag cgccaggcgc gcgcgagtgc tgattttctc gacggtctat
    25981 acttaagaga tgccaggcgg acttaacgac tggccgcaac aacagagtaa cggttgcgag
    26041 gaaagatgat gaaaaccgca aaagagtaca gcgataccgc aaaacgtgag gtcagcgtcg
    26101 atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt catcgcagcc
    26161 agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg cgtgaattgg
    26221 cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat cgttgaatgc
    26281 acaataaaaa aatcccgacc ctgagggggt cgggatgaaa cttgcttaag caagaagcac
    26341 ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatccg caattttttt
    26401 cgctgacaag aaaatattcg tgatatgaat gattaacgct acttatgaat agaaatgtga
    26461 cgcgcgtcac cccttggtgg ctctggcgtc aacgggatcg ctcccgaaaa aatcatcttt
    26521 gataatttgt gctgttttga gaatcatcct ggtaagggga gtactgcgca accatgcttt
    26581 caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg gacagtcata
    26641 gttatgactg gcaaccagct gccccctggc tcagccgttt acgcgaagcg aatgttcccg
    26701 tcattctctg tagcagtaag acatcagccg aaatgctgta cttgcaaaaa acgttggggc
    26761 tacaaggttt accgctgatt gccgaaaatg gcgcagtgat ccagcttgct gagcaatggc
    26821 aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa atcagccagg
    26881 ttttaaatac gctacgcgag aaagaacatt ttaaattcac aacttttgat gatgtcgacg
    26941 atgcaaccat cgccgaatgg acgggattaa gccgtagcca ggcggcgctg acgcagctac
    27001 atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcgtatggca caatttaccg
    27061 ctcgtctgaa cgaactgggc ttgcagttta tgcagggtgc gcgcttctgg cacgtcctgg
    27121 atgcctctgc cggaaaagat caggctgcca actggattat cgcgacctat caacaatcgt
    27181 caggcaaacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg cccttactgg
    27241 aggtaatgga ttacgcggtg attgtgaaag ggttaaaccg tgaaggggtg catctgcatg
    27301 atgaggatcc ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc tggcgtgaag
    27361 ggctggacca ttttttctcc gcccattaag cgttatcgct cgcgaatacc cgattgcgcc
    27421 cggcctgttt agcgagataa agtcgacggt cggccagtga ctggagttgt tcaaaatcat
    27481 aatcaccggt ttcctcgctg ctgcttaccc ccagcgaggc actgatgcgt atcgtcgtac
    27541 tcttggcgat caacatctct ttttcattta gcttcaggcg aatacgttct gcaacctccg
    27601 cagcctgcgt caggctcgcg cctggcagaa tcacacaaaa ctcctcacca ccgacccgcc
    27661 cggcaacatc ctgcgctcgc aaggaactgc taattaatcc ggcagcatga gaaagaacac
    27721 ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca aggtcgacct
    27781 gaatgacaga aaaaggatgt tggtgcgtct gacacaattt agcgagcgga cgggcttttt
    27841 cgaacagtgc gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc tgccagtgca
    27901 acgagctttg caaaacatac atgttgctga ccatccggcg aatcacatac caggagatga
    27961 gtaacatggt ggtaaagagc gcccacagca gggttaatgc aatgctgata ctgccgaaat
    28021 cgccgcgcac gccttcgctt agcgtatgga cgcgcaccag cacaccgtcg aaatggtcca
    28081 gacgttccca gctaacatag cgactgttca tacgaatgcc gccccgcgtg tcatgttcca
    28141 ttgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt ggatggtcag
    28201 gattggaaga ggtcaataat ctcagcttac tgtcatagag ctgatactca ccatcgaggt
    28261 ttttatcgat ggcgtttctt aaaaattgct gcatggtacg cacgggaata ctcatcccca
    28321 gcacgccata ccagtagttt tcactgtcta ccggaacact gacggtaacc tgcggttcag
    28381 tattgctggc gtgttccggt tgcgaagtga accagcgtac cgcgcggtga cgattttctc
    28441 gttgcgaatg gccgacaaac cagggttggg tgacatagcc gtaataacgc gttggtacat
    28501 tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc ggcacgcgag acatacatcg
    28561 cctgttcaac catcgacgag gagttgtgtg ccagtcgcag caagtaacca acttccagtg
    28621 cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggagattc ccctcgctga
    28681 ctaatgcatc cgacacacca ttgactggca gggtgcgtcg tcggttgagt tcgatttgcc
    28741 aggcgtgctc gtcgcgatgc tgttcgaact cggttacggc attacgcaga gaggtgaaat
    28801 ccagtggcgc tacgagagct tcgcgcatgc cattacgcaa aaagatcagt ttgtcgacgt
    28861 tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta cgctggctgg
    28921 agatataggc atcttccagc accaccactt cacgccaggt gagcaaggtg gaaaaaagca
    28981 ataccacgat aaagcagaga ttaacgacat gaccaggccc caggcggcgt gcgagttttt
    29041 tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact cccaacctgc
    29101 ttttttatga ttctggcata acctggcgat agcgtaccgc aaaacagcga taattttata
    29161 atggtggcat gataagacgc gacagacatc gatagtttta taacgtaggc atgataagac
    29221 gcgatagcgt cgcatcaggc aattcagtgc agacactggc acacccgacc ggatgcgacg
    29281 tgagcgccac atccggtcgg gtgttaatga taccctttca aacttcttcc gcagaacgac
    29341 tcccctcccc cggaacaagc tcggtttcat gaaaagcttc ccgcttgacc ccgtaaccgt
    29401 cataccaacg acactcaacc ataccgctgg agtatccggt gacaatcatc cgcgggccgc
    29461 cctctttaac cgtaacttcc tcactaacca taaagctcat actcgcctcc ttttttcgag
    29521 tgaaacttgt tcaccttagt tgaagatggc gaattttgca taatagcctg tgcgagtatt
    29581 aatgtgcctg accgcgtatt ttcgctacgg ctttcactcc cagcaccacg atgccgccga
    29641 tgataaatcc aagaatcaga tttaaaacag tcggtaatat catcgccacc actgcacttt
    29701 gctgcccggc gaaatgttca atggcgtgat gcagcggcgc aattccatgt accacaatcc
    29761 caccgccgac gaggaacatc gccagcgtgc cgacaatcga taacgccttc atcaaccagg
    29821 gcgcaatgat caataaccct ttgcctaatg cctgcatcag cgcgctggat ttttccgcca
    29881 gccaataccc caggtcatca atcttaacga taacccctac cagaccgtag acgcccacgg
    29941 tcactaccag cgcgatgcct gaaagcacca gcacctgatt aagcaacggc gcttcggcca
    30001 caatccccag cgtgatggcg acgatttccg cagacaatat aaaatcggta cgaatcgccc
    30061 cttttatttt gtctttttca aacttcagcg gatcctgcgc cgccagcttc tccagacgct
    30121 gctgactctg cgccggatct tctttatgtt tacgcgcctc cagcatatgc agcactttct
    30181 ctaccccttc aaagcagaga aacgcgccgc caatcatcaa cagtggcgta atcgcccacg
    30241 ggataaacgc actgatgatc agcgccagcg gcaccagaat cactttattg atcagcgatc
    30301 ctttcgccac gccccagacc acgggaagtt cccggttggc gcgcacgcct gcaacttgtt
    30361 gcgcattgag cgataagtca tcccctaata caccggcggt tttcttcgcc gccagtttgc
    30421 ccatcacgga gatatcgtcc agcagtgtgg cgatatcatc gagcaacgtc agtaaactac
    30481 tcccggccaa aagcactccc tctcattttt attgttgaag ggtgtaagta tgaagcaaaa
    30541 acggcagctc tgatacttgc ttcacaggtc aacatttatt taacaatcaa gtgcaattat
    30601 atctctcgcc agcagaatga ttttcacgtt tactatgaac caccttttta tttcagccgt
    30661 gagggattat gcgtttccgc cagttgttac cgctttttgg cgcgctgttt gcgttgtata
    30721 tcatttgggg ctcaacctat tttgtcattc ggattggcgt ggaaagctgg cctccgttaa
    30781 tgatggcggg cgttcgattc ctggcagccg gtattttatt gctggcattt ttgctactgc
    30841 gcggacacaa acttcccccg ctacgtccgc tgctcaatgc cgcgctgatt ggcctgttat
    30901 tgctggctgt cggtaatggc atggtgacgg ttgccgaaca tcaaaatgtt ccttccggca
    30961 tcgccgccgt tgtggttgca accgtgcccc tctttaccct gtgcttcagc cgcctgtttg
    31021 gcattaaaac gcgcaaactg gaatgggtgg gtatcgccat tgggcttgcc ggaatcatca
    31081 tgctcaatag cggtggaaat ttaagcggca atccgtgggg cgcgattctg attttaatcg
    31141 gctcgattag ctgggcgttt ggctcggttt atggctcgcg cattacctta cctgtaggga
    31201 tgatggcggg tgcgattgag atgctggcgg ctggcgtggt gttaatgatt gcgtcgatga
    31261 ttgcgggtga aaaactgacg gcgctccctt ccctttcagg cttccttgcg gtcggctatc
    31321 tggcgctgtt tggttcgatt atcgccatta acgcttatat gtatttaatc cgcaacgtca
    31381 gtccggctct cgccaccagc tacgcctacg ttaacccagt ggtcgcggtc ttgctgggta
    31441 cggggctggg tggcgaaacg ctgtcgaaga ttgaatggct ggcgctcggc gtaattgttt
    31501 tcgcggtggt actggtcacg ttgggaaaat atctcttccc ggccaaaccc gttgtcgcgc
    31561 cagttattcc ggatgcatca agcgagtaaa tgaatcccct gcgtgtcgat ctgcgcgctg
    31621 gcaccttcgc cgcagatcca ctcttccaga cgctcggtaa gcgccgcatc cgtcagcttc
    31681 tcgcgcccgc gtaacgcaca ttcccagaca atcaatacgc gccaaccgag ttcctgcaaa
    31741 tggctgatat cacgacgatc gcgctcgaca tttttaccta tcttctccag ccaaaaatct
    31801 gttcgcgtgg cggggacttt aaacaggtag caatggtgat ggtgccagaa gcaaccgtgg
    31861 gcaaatatca cacagcgaaa ttcatcaagg acaaaatccg ggcgtccggg cagactggcg
    31921 tcctgaacgc gaaacgccag gccctgccca gttaacaaac tggcgaggcg cttctcaatc
    31981 gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc gggtggcctt atcgtgaacg
    32041 tcgaccatgt tgtgcctctt gctgacgcaa cgccaccgcc tgtttgattt ttggctcaag
    32101 cagttttgcc accgcggcaa agaccggcac gaccaccgag ttaccgaact ggcgataagc
    32161 ctgagtgtcc gaaaccggaa tgcggaattt cgcttctccc ggcgcttcaa aacccattaa
    32221 gcgcgcacat tcccgaggcg ttaaccgacg tgggcgatgt tgctgattca gcggatcgtc
    32281 aaagtctttc tcacccgttg ccatatccca gccgcgatcg attaaaattt ccgcgccatc
    32341 tttgtaataa cgcgcagaca gcgtacgcgt gacgctttgc ggattgttcg gataaaccat
    32401 tccataaccg aagccgttac cgcgcgcctg atgttttttc gcatatcgat agaggtactt
    32461 ccacagtacc ggcgtcagga tatatttcgc ctcgaccatc gggtccagca gctgcgccag
    32521 cgtcactcgc tgtgcaggga aacatttgct gatatcacgc agagtaaaat cggctttcag
    32581 attcagatcg cgacgaaaac ccaccagcac gatgcgttca cggtgctgcg gcagaaaatg
    32641 tttgccgtcg atgattttcg gatcgtccgg cccgttatct tctgcatcag ccacgtcata
    32701 gcccagttcg tccagcgtct gcatgatgat gcggaacgtt ttaccctggt cgtgactttt
    32761 caggtttttg acgttttcga gcacaaacat cgccggacga cgcgcgtcga taatgcgcac
    32821 cacatcaaag aacagcgtgc cctgggtatc gcaggcaaaa ccgtgcgccc gcccgagcga
    32881 gttctttttc gatacgccag ccagcgaaaa tggctgacaa gggaaaccgg ccagtaaaac
    32941 atcgtgttca ggaatgtgtt gacgaatatg ttccgccgcc gcctcatcac tcacgccttc
    33001 ttgatggctg agggtgatgt cgcggatatc ctcattaaaa tgatgcgtcg ccgggtcgca
    33061 ataatggttg gctttataag tgcgtaccgc atgtttgttc cattcgctgg taaacacgca
    33121 ctgtccacca atcgattcaa aaccgcgacg gatgccgcca attccggcaa atagatcgat
    33181 aaagcgaaac gcataatgcg gatgatgtgc cggtggtttg ggtaacaacg tttgcagatg
    33241 ggcgaactct ttctcactta aacggtgcca tgccgagtca ttcgccagcg cacgttttaa
    33301 aattgccgcg ctccagtgat tctcacccac accattaagc tgcgccacca gcgttttaac
    33361 atcataaatt tgcagcagtt tctccagcat tgcctgtgcg gcattcccgg tgctgtatga
    33421 atcggttact gatatatttt cctgcataga tttaaccggc catctgagat ggccgacagg
    33481 ttaacataat tactgccgtt tagggccacg cgctcagcca gcaggtgaaa acgcatctat
    33541 caccttgtga tcgatacctt cattctcccc cgccagttcg gcaccgagct tcgccataaa
    33601 ctcgactaaa aattgcgcgt tgtgctgtgc cagctgcttg cctcttgcgg tttgcatggt
    33661 ttgcggtaat ttcagtaact ttgtctggaa atgatccagc gcgtagcgtt tatcatcaag
    33721 cgggcgatgc tgtgcgaacg gatcttcgcc atcaaacagc gccaagccca atgcccctga
    33781 aacggcaaat acgcgcgcca ggccaatcgc ccccagggct tccagccgat ccgcatcctg
    33841 gacgatttta gcttccgttg ttaagggggc gatttgcgcg ctgaaactgt gagcggcaat
    33901 ggcatgacaa acggcctcaa ttttctccgc cggaaattgc acgaactctt cggtaatgct
    33961 gccaacttac tgatttagtg tatgatggtg attttaaggt gcttgcgtgg cttccatttc
    34021 catcagatgt ccttcctgct ccgctactga aggcgtggtg cgtaacggca aaagcactgc
    34081 cggacatcag cgctatctct gctctcattg ccgtaaaaca tggcaactac agttcactta
    34141 caccgcct
//
LOCUS       DAZFRJ010000030        34030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000030 DAZFRJ010000000
VERSION     DAZFRJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.030"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..734
                     /gene="yadV"
                     /locus_tag="ACHKUN_001321"
     CDS             <1..734
                     /gene="yadV"
                     /locus_tag="ACHKUN_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6659618.1"
                     /translation="NTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRL
                     ENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLP
                     KDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALK
                     WFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNG
                     KASSAKVHFYAINDFGGAIEGNASL"
     gene            769..3369
                     /gene="htrE"
                     /locus_tag="ACHKUN_001322"
     CDS             769..3369
                     /gene="htrE"
                     /locus_tag="ACHKUN_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO6659619.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3386..3955
                     /gene="yadM"
                     /locus_tag="ACHKUN_001323"
     CDS             3386..3955
                     /gene="yadM"
                     /locus_tag="ACHKUN_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6659620.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            3970..4572
                     /gene="yadL"
                     /locus_tag="ACHKUN_001324"
     CDS             3970..4572
                     /gene="yadL"
                     /locus_tag="ACHKUN_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO6659621.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4599..5195
                     /locus_tag="ACHKUN_001325"
     CDS             4599..5195
                     /locus_tag="ACHKUN_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6659622.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5179..6522
                     /locus_tag="ACHKUN_001326"
                     /pseudo
     CDS             5179..6522
                     /locus_tag="ACHKUN_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            6635..7429
                     /gene="panB"
                     /locus_tag="ACHKUN_001327"
     CDS             6635..7429
                     /gene="panB"
                     /locus_tag="ACHKUN_001327"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO6659623.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7441..8292
                     /gene="panC"
                     /locus_tag="ACHKUN_001328"
     CDS             7441..8292
                     /gene="panC"
                     /locus_tag="ACHKUN_001328"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO6659624.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8366..9268)
                     /gene="rpnC"
                     /locus_tag="ACHKUN_001329"
     CDS             complement(8366..9268)
                     /gene="rpnC"
                     /locus_tag="ACHKUN_001329"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO6659625.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            9542..9922
                     /gene="panD"
                     /locus_tag="ACHKUN_001330"
     CDS             9542..9922
                     /gene="panD"
                     /locus_tag="ACHKUN_001330"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO6659626.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(9926..11155)
                     /gene="yadE"
                     /locus_tag="ACHKUN_001331"
     CDS             complement(9926..11155)
                     /gene="yadE"
                     /locus_tag="ACHKUN_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6659627.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11219..11659)
                     /gene="yadI"
                     /locus_tag="ACHKUN_001332"
     CDS             complement(11219..11659)
                     /gene="yadI"
                     /locus_tag="ACHKUN_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6659628.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11764..12534)
                     /gene="yadH"
                     /locus_tag="ACHKUN_001333"
     CDS             complement(11764..12534)
                     /gene="yadH"
                     /locus_tag="ACHKUN_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6659629.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12531..13457)
                     /gene="yadG"
                     /locus_tag="ACHKUN_001334"
     CDS             complement(12531..13457)
                     /gene="yadG"
                     /locus_tag="ACHKUN_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6659630.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13566..14228
                     /gene="can"
                     /locus_tag="ACHKUN_001335"
     CDS             13566..14228
                     /gene="can"
                     /locus_tag="ACHKUN_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO6659631.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14269..14805)
                     /gene="hpt"
                     /locus_tag="ACHKUN_001336"
     CDS             complement(14269..14805)
                     /gene="hpt"
                     /locus_tag="ACHKUN_001336"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO6659632.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15011..17401
                     /gene="gcd"
                     /locus_tag="ACHKUN_001337"
     CDS             15011..17401
                     /gene="gcd"
                     /locus_tag="ACHKUN_001337"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO6659633.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17448..18998)
                     /gene="cueO"
                     /locus_tag="ACHKUN_001338"
     CDS             complement(17448..18998)
                     /gene="cueO"
                     /locus_tag="ACHKUN_001338"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO6659634.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19164..19511
                     /gene="yacC"
                     /locus_tag="ACHKUN_001339"
     CDS             19164..19511
                     /gene="yacC"
                     /locus_tag="ACHKUN_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO6659635.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19617..20483
                     /gene="speE"
                     /locus_tag="ACHKUN_001340"
     CDS             19617..20483
                     /gene="speE"
                     /locus_tag="ACHKUN_001340"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO6659636.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            20499..21293
                     /gene="speD"
                     /locus_tag="ACHKUN_001341"
     CDS             20499..21293
                     /gene="speD"
                     /locus_tag="ACHKUN_001341"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO6659637.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21331..21693)
                     /gene="yacL"
                     /locus_tag="ACHKUN_001342"
     CDS             complement(21331..21693)
                     /gene="yacL"
                     /locus_tag="ACHKUN_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO6659638.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(21868..24465)
                     /gene="acnB"
                     /locus_tag="ACHKUN_001343"
     CDS             complement(21868..24465)
                     /gene="acnB"
                     /locus_tag="ACHKUN_001343"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO6659639.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            24820..26577
                     /gene="yacH"
                     /locus_tag="ACHKUN_001344"
     CDS             24820..26577
                     /gene="yacH"
                     /locus_tag="ACHKUN_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO6659640.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(26648..28072)
                     /gene="lpdA"
                     /locus_tag="ACHKUN_001345"
     CDS             complement(26648..28072)
                     /gene="lpdA"
                     /locus_tag="ACHKUN_001345"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6659641.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28397..30289)
                     /gene="aceF"
                     /locus_tag="ACHKUN_001346"
     CDS             complement(28397..30289)
                     /gene="aceF"
                     /locus_tag="ACHKUN_001346"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO6659642.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30304..32967)
                     /gene="aceE"
                     /locus_tag="ACHKUN_001347"
     CDS             complement(30304..32967)
                     /gene="aceE"
                     /locus_tag="ACHKUN_001347"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO6659643.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33128..33892)
                     /gene="pdhR"
                     /locus_tag="ACHKUN_001348"
     CDS             complement(33128..33892)
                     /gene="pdhR"
                     /locus_tag="ACHKUN_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO6659644.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8507 a         8877 c         8213 g         8433 t
ORIGIN      
        1 ttaacaccaa acatacaaca gctttatgct tcgtaacctg tatggctttt agttcatcgt
       61 ctattgcgga cattgtcatt tcgggtactc gcgtaatata taaaagcgat caaaaaagtg
      121 tcaacgtacg tctggaaaat aaagggaata acccgttgct tgtccagagt tggttagata
      181 ctggcgatga caacgctgag cctggcagta ttacagtccc ttttactgcc acgccgccag
      241 tatcgcgtat tgatgccaaa cgtgggcaaa caatcaaatt aatgtacaca gccagcacct
      301 cactgcctaa agacagagag agcgtgttct ggtttaacgt actggaagtt ccaccaaaac
      361 cagatgcaga aaaggtcgcg aatcaaagtc tgctgcaact ggcatttcgc acacgtataa
      421 aacttttcta tcgcccggat ggattgaagg gaaatccctc tgaagccccg ttagccctga
      481 agtggttctg gtcaggttca gaaggtaagg cgtcattgcg agtgaccaat ccaacccctt
      541 actacgtctc ttttagcagt ggtgatttag aagctagcgg taaacgctat ccgattgatg
      601 tgaaaatgat tgcaccattt agtgatgagg tcatgaaagt cactggcctt aatggcaaag
      661 caagttctgc aaaagtacat ttttacgcca ttaatgattt tggtggcgcc attgaaggta
      721 atgccagtct gtaatcaggc aaggatataa ttccgcagga agcataacgt gactataaaa
      781 ccaactaaaa attatcataa tcatctgacc cgtatcgcta cgttttgcgc gctgctgtat
      841 tgcaatagtg ctttctgtgc tgaacttgtt gaatatgacc ataccttcct gatggggcag
      901 aatgcctcaa atattgatct cagccggtat agtgaaggta accctgctat accgggtatg
      961 tacgacgtca gtgtttatgt caacgatcaa cctattatta accaaagcat tacatttatt
     1021 gaaattgaag gaaaaaaaaa cgcacaagcc tgtatcacac ttaagaattt attgcagttt
     1081 catataaatt ctcccgatat aaataacgaa aaagccgttc tgcttgccag ggatgaaaca
     1141 ctcggcaatt gccttaattt gacggaaatt atccctcagg cttctgttcg ttatgacgtt
     1201 aacgagcaac gtctggatat agacgttcct caggcatggg taatgaaaaa ttaccaaaac
     1261 tatgttgatc catcgttatg ggaaaatggc attaatgcgg ccatgttgtc atacaacctc
     1321 aacggatatc atagtgaaac tcctggtcga agaaatgaca gcatttatgc tgcttttaac
     1381 ggtgggatga atttaggtgc atggcgactg cgtgcctcag gcaactacaa ctggatgacc
     1441 gattctggca gtaattatga ttttaagaat cggtatatac agcgtgatat cgcctcgctg
     1501 cgttctcaac tcattcttgg tgagtcttat acaacgggcg aaacctttga ttctgtcagt
     1561 atccgcggca ttcgtttata cagcgacagc cgcatgttgc ctccgacatt agcgagtttt
     1621 gcgcctatca ttcatggcgt tgctaatacc aacgccaaag tcactattac gcaaggtggc
     1681 tataagattt atgaaacgac tgtgccgcca ggcgctttcg tcattgatga tctgagtccg
     1741 tcaggttacg gcagcgatct tattgttact gtcgaagaat ccgatggctc aaagcggaca
     1801 ttctcgcaac ctttttcatc ggtcgttcaa atgttacgcc ctggcgttgg acgttgggat
     1861 attagcggcg gccaggtctt aaaagatgat attcaggatg agcctaattt atttcaggcc
     1921 agctactact acggtctgaa taactatcta acgggttata ctggtattca gattaccgat
     1981 aataactata ccgctggttt gttaggtctt ggtctgaata cttcagtcgg tgcattttct
     2041 ttcgatgtga ctcactccaa tgttcgtata ccggatgata aaacatatca gggacaaagt
     2101 tatcgtgttt cctggaacaa gttattcgaa gaaacaagta cttcactgaa tatcgcggcc
     2161 tatcgctatt cgacacagaa ttaccttggt cttaatgatg cactaactct aattgatgaa
     2221 gtgaaacatc ccgaacaaga tcttgaaccg aaatccatgc gtaattactc gcgcatgaaa
     2281 aatcaggtta cggtcagtat taaccaaccg ctaaaatttg agaaaaaaga ttacggttca
     2341 ttttatcttt ccggaagttg gtccgattac tgggcttccg gacaaaatcg tagcaattac
     2401 tctattggct acagtaacag tgcatcctgg ggcagctaca gtgtcagtgc ccagcgttca
     2461 tggaatgaag acggcgacac tgacgatagc gtttatctca gtttcaccat tcctattgaa
     2521 aaattacttg gcactgaaca acggacttca ggtttccaga gtattgatac tcaaatgagc
     2581 agtgacttta agggtaataa ccaactcaac gtcagtagca gtggctatag tgataacgct
     2641 cgcgtcagtt atagcgtaaa tactggctat acgatgaata aagccagcaa agatttgagt
     2701 tatgttgggg gttatgccag ctatgaatca ccgtggggaa ccctggcagg ttcagtttct
     2761 gcaaatagcg ataacagtcg tcaagtgtct ctcagcaccg acggtggttt tgtattgcat
     2821 agcggcggcc tgaccttcag taatgacagt tttagcgact ccgatacact ggcggtagtt
     2881 caggctccag gtgctcaagg agcgcgaata aattatggca acagtactat cgatcgatgg
     2941 ggttatggtg tcaccagcgc tctttctcct tatcatgaaa accgtatcgc gctggatatc
     3001 aacgatcttg agaacgatgt tgaattaaaa agtaccagtg cagtagctgt accgcgtcag
     3061 ggttcagtcg tctttgctga ttttgaaacc gtgcaagggc aatcagccat tatgaacatc
     3121 acacgaagtg atggtaaaaa tattccattt gctgcagata tttatgatga gcaaggcaat
     3181 gtcattggta atgttggaca gggtggacaa gcatttgttc gtggtattga gcagcaggga
     3241 aatatcagca ttaaatggct cgaagaaagt aaacccgtaa gttgtcttgc gcattatcaa
     3301 caaagttcag aagcagaaaa aatagcgcaa tctattattc tgaatggaat tcggtgtcag
     3361 attcagtaac tacaagggac gtcaaatgat aaaaataacg ccacataaaa tagcgatact
     3421 gatgggaata ttattatcac catcagtatt tgcaacggat attaatgtag agtttacagc
     3481 cactgtcaaa gcgacaacct gtaacatcac acttactggt aataacgtca cgaatgatgg
     3541 caataataac tacacattga gaatccctaa gatgggtctg gataagatcg cgaataaaac
     3601 gacagaatct caggctgatt ttaaactggt tgctagtggg tgcagcagtg gcatcagttg
     3661 gattgatacc actctgagcg gaaatgcatc atcaagctca cctaagctta ttataccgca
     3721 gtctggtgat tcatcttcga cgacaagtaa tatcggtatg ggtttcaaaa aacggactac
     3781 tgatgatgcc actttcctta aacctaacag tgcggaaaag atacgctgga gcacagacga
     3841 gatgcagccc gataagggtc ttgaaatgac cgttgcgcta cgtgaaacag atgcagggca
     3901 aggcgtaccg gggaattttc gtgcactggc aacgtttaat ttcatctatc aataagaagg
     3961 ataattaaga tgacatttaa aaatttacgt tatggattat ccagcagtgt cgttttggct
     4021 gcctcattgt tcagcgtacc ctcttatgcg gcaacagaca gcattgggct gaccgtgatt
     4081 actaccgtag aaataggtac ttgtaccgct acattagtaa atgactctga tcaggacatt
     4141 tctgttgttg actttggcga tgtatatatt tctgaaatca atgccaagac caaagttaaa
     4201 acattcaaac tcaaattcaa agactgtgcg ggtatcccca ataaaaaagc acaaataaaa
     4261 ttaaccaagc gagccacatg cgaaggtact gctaatgacg gtgcggggtt cgcaaatgga
     4321 tccacagccg cagataaagc aagtgccgtc gccgttgaag tctggagcac cgaaactccg
     4381 gcaacaggga gcgcaacaca atttagttgt gtgacaccag catctcaaga ggtcactatc
     4441 tccaatgcag ccaacgcggt tgtttattat ccgatgagtg ctcgactggt cgtcgaaaaa
     4501 aataaaaccg taagcaatgt cactgcgggt aaattttctg cacctgccac atttacagta
     4561 acctataact aacagactga cagatccagg aactatatat gcatcccact caacgtaagc
     4621 tgatgaagag aataattctg tttctgtcac tacagtttcc cattacctat ccagccatcg
     4681 ctgggcagga tattgacctt gttgccaatg taaaaaacag cacttgcaaa agcggaatca
     4741 gtaaccaggg taatattgat cttggcgtcg ttggggtggg atatttttca gataatgtta
     4801 ctcctgaaag ctatcaacca ggtggaaaag agttcactgt cacagtatca gactgtgcat
     4861 tacagggaac tggcgatgtg cttaatcagt tacatattga ttttagagcc cttagcggtg
     4921 tcatggctgc aggttcaagg caaatatttg ccaatgaagt cgcaaccggg gctaaaaatg
     4981 tcggtgtcgt tctcttttcc attcaggacc cgaccaatat attcaatgtt attagttcag
     5041 caggaaattc tcgttccgtg tatccagtaa tgacttctgc cctgcataat tcttcatgga
     5101 aattttatgc cagaatgcaa aaaatcgatc ccgcgctgga tgtcatatct ggccaggtaa
     5161 tgagtcatat attagttgat gtgtactacg aataatagat caaaacaaat aactgcgact
     5221 gttacagcag tagatggtgg agtgataatg aagaaattct tcagacattt tcttttttta
     5281 attctgtgct tgagttgtta taccgcgagt gctggcactg atgataacgt aggatatatt
     5341 gttgggaata gttacggcgt tggtccgtca gatcaaaaat ggagagaaac ggggccgaat
     5401 ggcgatgcta ctgtcatatt cagatatgca acaagtacaa ataatttagt tttctacaaa
     5461 ccgacgcagc ttggacctac aggtgtaaaa ttacagtgga gtcagttaga taccgcttct
     5521 ggtggtggtt ttctttattg caacagaagt gatagcacaa gtggtagcgc aatgcgtatt
     5581 gaaaatgcaa tggttgactc aggtaaaatg tatggctccc ataaattatt taatacatca
     5641 gttcctggtt tgtattacac attattaatt tcaaacatgt ggtcagctta cggtaccgta
     5701 actaacgtta gttcacctgg gatatacatt ggtgactctg cagaacaata tttttcgtgg
     5761 tataatccaa gcgaagacgt gttatattag agttgcaata atgcgaatag cacccgtaaa
     5821 tactgggctg taggtggtat ttatcagacc cttacaattg aattctatac agatacaaac
     5881 tttgatccaa cagtaactca acagatcaaa ttatccagct catcaaatta tctgtattca
     5941 tttaaagcct atggcgcagg ccaaggtata aatgagcata gttattttat caaaatcgat
     6001 ttcgatctgc tcaatgtcaa gttgactaat cccacttgtt ttaccgctat gcttagtgga
     6061 acttctgtga ctggttctac ggtaaaaatg ggtgaatata gtgcagaaca aatcagaaac
     6121 ggtgccacac cggttccttt tgatatttca cttcaaaact gcgttcgtgt gactaatatt
     6181 gagacaaaat tagtttcaac aaaggttggt actgaaaacg ggcaactcct tggtaatact
     6241 ctcacaggta atgacgcagc aaaaggagtc ggtgtactca tagaaggttt agcaactagt
     6301 aaaaatcctc taatgacatt gaaacctaat gattcaaatt ctgtttataa agattacgac
     6361 ccgagaggca aagacgatac aacaggagga gtttacccgg atcaagatac aggtataaca
     6421 taccctctcc atttccaggc cacgctacaa caggatggaa ctataccaat agaagctggt
     6481 gagtttaaag ccaccagtac tttccaggta acctaccctt aataagtcca ccgcaccgcc
     6541 atcatctggc ggtgcagtaa ttgataaatc attgccccca ccaatgtgac tgataaaatg
     6601 ccaggcaaca cgacatcatt tatcaggata cgttatgaaa ccaaccacca ttgcttcact
     6661 tcagaaatgt aagcaggata aaaagcgttt cgcgaccatc accgcttacg actacagctt
     6721 cgcgaaacta tttgctgatg aaggacttaa cgtcatgctg gtaggcgact cgctgggcat
     6781 gacggttcag ggtcacgact ccaccctgcc ggtgaccgtt gctgatatcg cctaccacac
     6841 tgccgccgta cgtcgcggcg cgccaaactg cctgctgctg gctgatctgc cgtttatggc
     6901 gtatgccacg ccggaacaag cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa
     6961 tatggtcaaa attgagggcg gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg
     7021 tgccgttcct gtatgtggtc acttaggttt aaccccacag tcagtgaata ttttcggtgg
     7081 ctacaaagtt caggggcgcg gcgatgaagc gggcgatcaa ctgctcagcg atgcattagc
     7141 cttagaagcc gctggggcac agctgctggt gctggaatgc gtgccggttg aactggcaaa
     7201 acgtattacc gatgcactgg cgatcccggt tattggcatt ggcgcaggca acgtcactga
     7261 cgggcagatc ctcgtgatgc acgacgcctt cggcattacc ggcggtcaca ttcctaaatt
     7321 cgctaaaaat ttcctcgccg aaacgggcga catccgcgcg gctgtgcggc agtatatggc
     7381 tgaagtggag tccggcgttt atccgggcga agaacacagt ttccattaag gagtcacgtt
     7441 gtgttaatta tcgaaaccct gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa
     7501 ggcaagcgcg tggcgctggt gcctactatg ggtaatctgc acgatggcca tatgaagctg
     7561 gtcgacgaag ccaaagcccg cgccgatgtg gtcgtcgtca gtattttcgt taacccaatg
     7621 cagtttgacc gcccggaaga tctggctcgt tatccacgga ccttgcaaga ggactgcgaa
     7681 aagctaaaca aacgtaaagt ggatttagtt ttcgcccctt cggtaaaaga gatctacccg
     7741 aacggtactg aaacccacac ttacgttgac gttcctggcc tttcgaccat gctggaaggt
     7801 gccagccgtc cgggacattt tcgcggcgtt tcgactattg tcagcaagct gttcaacctg
     7861 gtccagccgg acatcgcctg cttcggtgaa aaggactttc agcaactggc gctaatccgc
     7921 aaaatggttg ccgatatggg cttcgatatt gaaattgtcg gcgtcccgat tatacgcgcc
     7981 aaagatggcc ttgcgctcag ttcccgtaat ggttatctga cggcggaaca acgcaaaatt
     8041 gcgcccggtc tgtacaaagt tttaagttca attgccgaca aattgcaggc tggggaacgg
     8101 gatctcgatg aaattattac cattgcgggg caagaactga atgaaaaagg cttccgcgcc
     8161 gatgatattc agattcgcga tgccgacaca ttgctggaag tttctgaaac cagcaaacgg
     8221 gcagtaattc tggtagccgc ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc
     8281 gagctggcgt aatacttaac tggcgctacg gctgatggcg ccagttatta atttacccca
     8341 cgtcatctgt cataacacag gtaacttaaa taaggttaat taccttatca atctcagcaa
     8401 gaggtaaatt tgccatctct gcaacgtctt cccgagacat tcctttactt agaagacgca
     8461 gggcgaattc ttgcctttcc tcctgtcttc cctgctgaat ccccttctca atccctttct
     8521 cttcaaacca ctgcgccagc gtcatcatag actcccctcc cgtttccctg tctctcagca
     8581 caccataaaa caaatccgct tgttcagtat gaccgcgttg cagcatatag ttttgcatgg
     8641 caactaactg acttccgcta gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca
     8701 ataacattaa gtcgcgctgg cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc
     8761 gatgttgcat gatttcgtca tccggtgtga tggtgatatc caccagcggg aaaggactgt
     8821 tatagacgcg tcgtgccagc tccggcgagt aaaatatatc aaaccagcac attgagagcg
     8881 gataaggtgt ggcctcgccc tgataaaaca gtatcggcac caccagcggc agcttatcgt
     8941 gatccgcctc cagatgccgg tgcatggcag caatagaata acgcatcatg cgaaaggcca
     9001 ttttcttatc cggcttgctt tggtgttcaa tcacaacatg cagataaccg ggattgccct
     9061 gcatttgcac ggaatagagc acgtccgtgc tgtgtccttt caggctctct tcaatgaaac
     9121 tccccgactc taaatgaagc gtgttgaggt cacaaagttc tcgtaattcc actggcaaat
     9181 gtatctccag aaagtcgcga gccgtctccg cgtgcattaa aaattgttta aacactgcat
     9241 catgcggtgt ggtactcggt gcatccatct tccctgcctt ccgtgttgtc ttgcgtgaag
     9301 gcagtatcca gtcaaggcgt gccatcgtca gccacctctt tagtgtttac cgagcagcgt
     9361 tcagagaaat ttcaattggt tacagccgtt acatggcacg cttcggcacg gtacgcaaac
     9421 tgctgatgta aatgccgtca gatgagtaga cactaaacaa aaatcgggca atactgcgtg
     9481 agaatttcct caaagcaggc atcgcctgct tcgttaacga cagggtagaa aggtagaagt
     9541 tatgattcgc acgatgctgc agggcaaact ccaccgcgtg aaagtgactc atgcggacct
     9601 gcactatgaa ggttcttgcg ccattgacca ggattttctt gacgcagccg gtattctcga
     9661 aaacgaagcc attgatatct ggaatgtcac caacggcaag cgtttctcca cttatgccat
     9721 cgcggcagaa cgcggttcga gaattatttc tgttaacggt gcggcggccc actgcgccag
     9781 tgtcggcgat attgtcatca tcgccagctt cgttaccatg ccagatgaag aagctcgcac
     9841 ctggcgaccc aacgtcgcct attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc
     9901 gattccggta caggttgctt gattgttatc cctgcggctg gttactcacc agccgcgaca
     9961 tcgtctccag cgaatccgtt cttaagatat aaagtcgttt tagtaacaac ggattatccc
    10021 ccggttttac tttgcctttc atggttgtca ccgccaggtg aaatccggca tcgtttgctg
    10081 ccttcacggc tttgtcatta aatccgccaa acggatacga aagataccag acatgcggat
    10141 taaattgcgc cagagcgcgg cgtgaacgtg caaaatcaaa cagaatattg tgctcactac
    10201 ggctcagtaa tatgggtcgg cgatagccat ccacccgatg caaaaaatgg gtatgtgact
    10261 gaaaatcaaa aacatcgcga atttcgttaa gctcagaaac gctcataaat tgcagcgatt
    10321 ttgggttcca cttctgcggg tgacgtttga tgcgcgaggt aacaacaaac gccgtcgcct
    10381 tcatgccata ttgtttcaat acaggatacg catagcggct caccgacttg aggccatcat
    10441 caaaggtaat caccaccgct cgcgcaggga gattgatctt attcttcacg taaccttcca
    10501 gctgcgccat gctcagtgtc gcgtatcccc tgtcgcgcag ccaggccatt tggttattga
    10561 aagcgcgtac cgaggtggtc gtcgaagtat ggcgaaaacg ggtgttttct tcatcgcgca
    10621 gaatatggtg ataggttagc accggcaggc cattatcggg ttgggcatcc agcgcgctga
    10681 tataggcgag tcgatcgcca atacggatct gataccaggt ttgatttaac ctgtctttca
    10741 gtttatgcaa aatcgggtag cgcaaattgt ccgccagtac cccaaatggc gcactgcccg
    10801 cactcggcgc gttatagacc ggcgtatctt tccaggtaat taagttctga ttactcagcg
    10861 gcttgttgag gtcacccaaa ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat
    10921 gccctttatc gataaaccct ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag
    10981 tgggctccac ggcaatgatt tgcccggccc gaatattccc cacggtcacc atcttgtcgc
    11041 caatttgcgc ccagatcgca gcattttcga tggtttgcat ataacgggca ggtaacgcgg
    11101 cactgacact tgcggtaaac agcatcagca ggagaataac agcttgtttg tacatgaaaa
    11161 aataaccgag ggtgaaacaa cgcttttatt gtagcaaaag cgctataaga atcgtgggtt
    11221 aatactcata caaattatgt ttgaggacga aaggcggatt tttttgttgt tggtgccaaa
    11281 gactactcac ctccggccca cccagttcga caatacgctc gcgaaactct gaactggtca
    11341 tggtttcacg gcaagccatc atctgttcaa ttaacggtaa cgtgacacca gaaatcactt
    11401 cacaacggga gtgtttatgg cttaataatg aagccacgcg gtacggtggc gctccggcta
    11461 aatcggttaa gaagatgaca ccctcaccag agtccgcttc atgcagagca tcgcacatca
    11521 tgcggctgag catattagag cttaatccgc gccagaaatt cacggcccgg cactgaagaa
    11581 gtgccccatg ttttttctcc agcgcatcca gtatctcttg cgcccggtca tcgtgacagg
    11641 taattaccca acctaacatc gccttcctcc ttagccgaaa atcagtctac cctgaccatt
    11701 gttgataaag ggtgatagcc gtcaaaactc ctctcccccg aatccagagg agaggaaata
    11761 gccttagcta cgcaaaccac gtccacgttg gatcagcgac caacagatca aataaaacgc
    11821 cacaataaag accaccagta cgccaaaggt agtgaccagc ggcacatcat ttataccgag
    11881 gaagccgtag cggaaaccac tgatcatata aacgattggg ttcaggtgcg acagcccttg
    11941 ccagaacggc ggcaacaaag tcagggagta aaacaccccg cccaaatacg tgagtggcgt
    12001 taacacaaag gttggcacca ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag
    12061 caaacccgca agggagaata gcaccgccgt gagcaccagg gttaaggcaa cgaataccca
    12121 cgaatgcacc tgaaacggca caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa
    12181 cagaccacgc gccacgccac cgccgacata tccggcaata atgacgtgag tcggaaccgg
    12241 cgctaccagc agctcttcaa tattacgctg gaacttggca ccaaaaaatg atgacgcgac
    12301 gttagcgtag gcattggtga tcaccgacat catgatcagc cccggtacga tgaactgcat
    12361 atagctgaag ccatgcatat cgccaatacg cgaacctatc aggttaccga agataataaa
    12421 gtaaagggtc atggtgatga ctggcggcac cagcgtctgc acccagatac gcataaagcg
    12481 atggatctct ttcgcccaga tgctttttag cgccacccag taaagatgca tcatgcgcga
    12541 tctccttgtt tttcattaac cagtgaaaca aacagctctt ccagacggtt agctttgtta
    12601 cgcatactta atacctgaat gccctgctca cttaactgcg taaatacgct gttgatcccc
    12661 tgctcacgca gcacttcaac ttccagcgtc gcggtatcga ccagtcgata ctgatagcca
    12721 tcgagcttcg gtaacggact tttcggtgcg agatcgagaa taaaggtttc cgatttcagc
    12781 ttagccagca gcgccttcat cgaggtattt tccaccagct caccgtgttg aataatgccg
    12841 atattgcggc acagcatttc tgcttcttcc aggtagtgtg tggtgagaat gatggtggtg
    12901 cctttgtcgt ttaaatcctt caaaaagccc cacattgagc ggcgaagttc aatatccacg
    12961 cctgcggtcg gttcgtcgag aatcagtagt ttaggttcat gcattaacgc acgggcaatc
    13021 attaaacggc gcttcatccc gccagataac atacgcgcac gttcgttgcg ttttccccat
    13081 agatcgagct gcttaagata tttttcgctg cggatgtacg cctctttgcg ctccacaccg
    13141 tagtaccctg cctgattcac cacaatttgc tgcacggttt caaacgggtt gaagttaaat
    13201 tcctgcggca ccagtcccaa ctgacgttta gcgttcacga catccttctc gagatcgtaa
    13261 ccaaatacgc tgacccgccc ggaggtttta tttaccagag agctgataat accgatagtg
    13321 gtcgatttcc cggccccgtt cggcccgaga agcgcataaa aatcacccgc ttcgacctgc
    13381 aaatctatcc cacgaagcgc ctgaacgccg cctggatagg tttttttaag ctgttgaagt
    13441 tccagtgcaa tggtcataaa tttttactta ccttacgttc ttacacttta tctgtggttt
    13501 aaatcgtccc ggagttgccc tatattagcc aaacgtaatt atttggttac aggtcgttaa
    13561 cctccatgaa agacatagat acactcatca gcaacaatgc actatggtca aaaatgctgg
    13621 tggaagagga tcccgggttt tttgagaaac tggcacaagc gcaaaaaccg cgctttctat
    13681 ggattggatg ttccgacagt cgcgttcctg cagaacgttt aaccggtctt gagccgggcg
    13741 aactctttgt tcaccgtaat gttgctaacc tggtcattca cactgacctg aactgccttt
    13801 ccgtggttca gtatgcagtg gatgtactcg aagttgaaca cattattatc tgtggccact
    13861 acggttgcgg cggcgtacaa gccgcagttg aaaacccgga actggggctt atcaacaact
    13921 ggctgctgca tatccgcgat atctggttca aacatagctc attgctcggc gaaatgccgc
    13981 aagagcgccg tctggatacc ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg
    14041 gccactccac cattatgcaa tcagcgtgga aacgcgggca gaaagttacc attcacggct
    14101 gggcctacgg cattcacgac ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg
    14161 aaacccttga gcaacgttac cgtcacggga tttccaacct caagctgaaa cacgccaacc
    14221 acaaataaaa atgccatgcc ggatgcaaca catccggcaa cttcacactt actcgtccag
    14281 cagaatcact ttgccgatat acggcagatg acggtaacgc tgtgcgtaat caatgccgta
    14341 acccaccaca aactcatccg ggatcgagaa accgataaat tctaccggga cgttcacttc
    14401 acgacgggac ggtttatcca gcagcgtaca aatcgccagc gacttcggtt cgcgcaggct
    14461 taagatctca cgcactttcg acagtgtatt ccccgagtcg atgatatctt caacaatcag
    14521 cacgtccttg ccacggatat cttcatccag atctttgagg attttcacat cacgggtggt
    14581 ggacatgccg ctaccgtagc tggaggcggt cataaagtcg acttcatgag atacctgaac
    14641 ttcacggcac aggtccgcca taaacataaa tgagccacgc agcagaccca ccagcaccat
    14701 atcgctgccg ctgtctttgt aacgctcagt aatctgacga cccagttcgg cgatacgcgc
    14761 tttaatctcc gcttcgggga tcattacttc tacagtatgt ttcatatctc taaccatatg
    14821 atttaaaaac aaatcacttc gcacgggcag gttttggcac cgcaacaaag cgcaagacac
    14881 gcagtatacc agtaaaacta ttcattcgcg gaatctgtta ataaagctcg aattgtgatg
    14941 acgatcacac atgttaaaac ctataattaa ttgctactaa aatattaatg aattgaaatg
    15001 gtgtctcttt atggcaatta acaatacagg ctcgcgacga ttactcgtca cgctaacagc
    15061 cctttttgca gcgctttgcg ggctgtatct actcattggc ggaggctggc tggtcgcgat
    15121 tggcggctcc tggtactacc ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct
    15181 gtggcgcagt aaacgtgccg cgctttggct atacgccgcc ctgctgctcg gcaccatgat
    15241 ttggggcgtc tgggaagttg gtttcgactt ctgggcgctg actccgcgca gcgatattct
    15301 ggtcttcttc ggcatctggc tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc
    15361 cagcggcgca gttgccgcac tggtggtggc actgctgatt agcggtagta tcctgacctg
    15421 ggccggattt aacgatccgc aggaaatcaa cggcacctta agcgccgatg ccacacctgc
    15481 ggaagctatc tcacccgttg ccgatcagga ctggcctgcc tatggtcgca atcaggaagg
    15541 tcaacgcttt tcacctctga aacaaattaa cgccgataac gtccataatc tgaaagaagc
    15601 ctgggtgttc cgtactggcg atgtgaagca gccgaacgat ccgggcgaaa tcaccaatga
    15661 agtgacgccg attaaagtgg gcgacaccct ttacctgtgt accgctcacc agcgcctgtt
    15721 tgcgctcgat gccgccagcg gcaaagagaa atggcattac gatcctgagc tgaaaactaa
    15781 cgagtctttc cagcacgtaa cctgccgtgg cgtctcttat catgaagcca aagcagaaac
    15841 ggcttcgccg gaagtgatgg cggattgccc gcgtcgtatc attcttccgg tcaatgatgg
    15901 tcgactgatt gcgattaacg ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg
    15961 cgtgctcaat ctgcaaagca atatgccaga caccaaaccg ggtctgtatg aaccgacttc
    16021 gccaccgatt atcaccgata aaaccatcgt gatggcaggt tcagttaccg ataacttctc
    16081 aacccgcgaa acgtctggcg tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg
    16141 ggcttttgat cccggcgcga aagatccgaa cgcaatcccg tctgacgaac acacctttac
    16201 ctttaactcg ccaaactcgt gggcgccagc ggcctatgac gcgaagctgg atctggtcta
    16261 tctgccgatg ggcgtgacca cgccggatat ctggggcggt aaccgcacac cggaacagga
    16321 acgttatgcc agctcgattc tggcgctgaa tgccactacc gggaaactgg cgtggagcta
    16381 ccagaccgtt caccacgacc tgtgggacat ggatcttccg gcacagccga cgctggcgga
    16441 catcaccgtt aatggtcaga aagtgccagt tatttacgct ccggcgaaaa ccggcaacat
    16501 ttttgtgctc gatcgtcgca atggcgaact ggtggttccg gccccggaaa aaccggtgcc
    16561 gcaaggtgct gcgaaaggcg attacgttac tccgacccag ccgttctctg aactgagctt
    16621 ccgtccgaag aaagatttga gcggtgcgga tatgtgggga gccaccatgt ttgaccaact
    16681 ggtgtgccgc gtgatgttcc accagatgcg ctatgaaggc attttcacgc cgccatctga
    16741 acagggcacg ctggtcttcc cgggtaacct gggaatgttt gaatggggcg ggatttccgt
    16801 tgatccaaat cgtgaagtgg caattgccaa cccaatggca ctgccgtttg tttcgaaact
    16861 gatcccacgc ggtccgggta acccgatgga gcagccgaaa gatgccaaag gcacgggtac
    16921 ggaatccggc attcagccac agtacggtgt accgtatggt gtcacgctca acccgttcct
    16981 ctcaccgttt ggtctgccgt gtaaacagcc agcatggggt tatatctcgg cgctggatct
    17041 gaaaaccaat gaagtggtgt ggaagaaacg tattggtacg ccgcaagaca gtatgccgtt
    17101 cccgatgcct gtaccggtgc cgttcaatat gggtatgccg atgctgggcg ggccaatctc
    17161 cacggcgggt aacgtgctgt ttatcgctgc tacggcagat aactacctgc gcgcttacaa
    17221 catgagcaac ggtgaaaaac tgtggcaggg ccgtttacca gcgggtggtc aggcgacgcc
    17281 aatgacctat gaagtgaatg gcaagcagta tgtggtgatc tccgcaggcg gtcacggttc
    17341 atttggtacg aagatgggcg actatattgt ggcttatgcg ctgccggatg atgtgaaata
    17401 accataatgc ccggagagat ccgggcatat ttccgaatac ggtcttttta taccgtaaac
    17461 cctaacatca tccccgtatc ttcatgctcc agcagatggc agtgcgccat ataagcatgt
    17521 tctttcggtg cgttgtgatt aaacttcacc agcacttcac tgacattgcc ttctacctta
    17581 acggtatctt tccagcccgc gcgatgagcc gctggcggtt tgccattttc tgacaagata
    17641 cggaactggg tgccgtgaat atggaacgga tgcagcatca tgtcgcccac gccagagata
    17701 acccaacgtt cgtactgccc tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc
    17761 tgaccgttga ttttattggc atggtggaaa tcgaacttcc cgccatggtt catatgattc
    17821 atattgccgt gccccatatg gcccatcatc tggctatgat ccatcccggc catcgcctga
    17881 tcgccatatt tctccatcag catctgcatc cccatcatat cgagcatcgg gtccatagaa
    17941 agttgcagct tgcgtaccgt cagcccttcc agcgaaggta acgcaggcag gctacttaat
    18001 gtgtctggca aagcaccgga ggcactaata gcaatcggct gaatccgcat taccggatgc
    18061 ggcttgtcaa acggcgcaat cgccatcccc atctggctga ccggtagcgt caccaggtca
    18121 aagggtttgt tgtcgttaac ctccaccagc acttcaaaac gctcgcccat cagcaccggc
    18181 agctcgctca ccttcaccgg ttcaggtagc agaccaccgt cgctggcaat cacatacagc
    18241 gggcgattgt cgctggtggc gaaattgagc gagcgggcat tacagccatt aagcaaacgc
    18301 aggcgcagcc aaccacgcgg ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc
    18361 gtatcgccaa accagcccac ggcggcggtc atcacatcca gttgataatc aatctgccca
    18421 tcagcgttaa attttttatc ctgaacaatc accggaacat catcgatgcc ccactgtttt
    18481 ggcagcatta atttcaggat ctcgtcatct tcaattacca ccagcccagc caaccccatc
    18541 gccacctgtc gcccggtctt gccatgttga tgtggatgga accagcaagt agcggcaggt
    18601 tgatcaacgt tcaacgtcac cgagcgcttg ccacctggca gaataattcc ctgcgggccg
    18661 ccgtcgactt cacccggtac ttccagcccg tgccagtgca acgtcgtctc ttccgtcagt
    18721 tggttataga tatcaaccgt taccgctttg ccgcgctgta atttcaccgc cggccccagc
    18781 agattgccgt tatagcccca ggtagttgca gttttctcgc caaaggtaga ctgacctgcg
    18841 ccaatagtta actgaatgcg attacgggca tcggtcgtga gcaaatcagg gatcggtaac
    18901 gttgggcgtt ccgccgcaaa gactgcgcgg ctccacagcg gcaaggctga agccacaccc
    18961 agcgcgacgg aatattttaa gaaatcacga cgttgcatag ttatttcctt attcttaagc
    19021 aggcgaaaca aaatcaaacg ttgagcatag tccttcccct tacgggaagg tcaagccgca
    19081 gacacaattt aatcgacaat aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct
    19141 tctttaagcc cctggtagaa gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc
    19201 tgatggccgt ttgcgcaaac agttacgcgc tcagcgagtc tgaagccgaa gatatggccg
    19261 atttaacggc agtttttgtc tttctgaaga acgattgtgg ttaccagaac ttacctaacg
    19321 ggcaaattcg tcgcgcactg gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt
    19381 acgacacctt cgacatgaaa gccctcggtg aagacagcta ccgcgatctc agcggcattg
    19441 gcattcccgt cgctaaaaag tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct
    19501 acgtcaaata atccctgata cctttttgta gaaatattga ccgtgcatcc gcggtcaatg
    19561 ttagctatta tgttgcgccc tttttttacg ggtgttaaca aaggaggtat caacccatgg
    19621 ccgaaaaaaa acagtggcat gaaacgctac acgaccagtt tgggcagtac tttgcggtag
    19681 ataacgttct gtatcatgaa aagaccgatc accaggatct gatcattttt gagaacgctg
    19741 catttggtcg cgtaatggcg ctggatggcg tagtacaaac caccgagcgc gacgagttta
    19801 tctatcatga gatgatgacc catgttccgc tactggccca cggtcacgcg aaacatgtgc
    19861 tgattatcgg cggcggcgac ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg
    19921 agtcaatcac gatggtggaa atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc
    19981 ccaaccataa cgccggtagc tacgacgatc cgcgctttaa gctggtgatc gacgatggcg
    20041 tcaatttcgt taatcaaacc agccagacct ttgatgtcat tatctccgac tgcaccgatc
    20101 ctatcggtcc cggcgaaagt ctgttcacct cagcatttta tgaaggctgc aaacgttgcc
    20161 tgaatcctgg cggtatcttc gtcgcacaaa acggcgtctg ctttttacag caggaagaag
    20221 ccatcgacag ccatcgcaaa ctcagccatt acttcagcga cgttggcttt tatcaggcgg
    20281 cgatcccgac ctattacggc ggtatcatga cttttgcatg ggcgacagat aacgacgcct
    20341 tacgccatct ctcaaccgaa attattcagg cgcgttttct cgcttctggc ctgaaatgcc
    20401 gttattacaa tccggcaatc catacggcag cttttgcctt acctcagtat ctgcaagacg
    20461 cactggcttc acagccgtcc taaggagaag ataagaaatt gaaaaaactg aaactgcatg
    20521 gctttaataa tctgaccaaa agtctgagtt tttgtattta cgatatctgc tacgccaaaa
    20581 ctgccgaaga gcgcgacggt tatattgctt atatcgatga actctataat gccaaccgtc
    20641 tgaccgaaat cctgtcagaa acctgttcca ttatcggggc caatattctt aacatcgccc
    20701 gccaggatta cgaaccacag ggtgccagcg tcactattct ggtgagtgaa gaaccggttg
    20761 acccgaaact catcgacaaa acagaacacc ccggcccact gccagaaacg gtcgttgccc
    20821 atctcgataa aagtcatatt tgcgtacata cctacccgga aagtcatcct gaaggcggtt
    20881 tatgtacctt ccgcgccgat attgaagtct ctacctgcgg cgtgatttct ccgctgaagg
    20941 cgctgaatta cctgatccac cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc
    21001 gcggttttac ccgcgacatt aacggtatga agcactttat cgaccatgag attaattcga
    21061 ttcagaactt tatgtctgac gatatgaagg cgctgtatga catggtggat gtgaacgtct
    21121 atcaggaaaa tatcttccat accaagatgt tgcttaaaga gttcgacctt aagcactaca
    21181 tgttccacac caaaccggaa gatttaaccg acagcgagcg ccaggaaatt accgctgcgc
    21241 tgtggaaaga aatgcgcgag atttattacg ggcgcaatat gccagctgtt taacggctct
    21301 ggcggagctc ccaggctccg ccagatttat ttacttctgc tgcacgaaat tgcggtaagc
    21361 cgccacgacc tgcagaaaat cctcaacgcc gcatagcgac aggctttctt cgtcgtagta
    21421 gttcatcccc tcttccattt catcgccagc gaattccagt tgattggcgc gaaccatcac
    21481 ctcttcaccg tccatccaca gggtgtattc atgccctgcc cgttgccagg accgttcgct
    21541 acctttcagt gcgtgcgctg cttgttccac ttcatcaagc aaggccaggt tttctttcac
    21601 ctcttcatta aaccagtgcc cgaccacttc atgccccatg gacatacgca cctttaccac
    21661 tccggtaata tcgcgcagaa attcgtaatc catagtgtgt tcctctgctc ccggcaatgc
    21721 gccgaaatcg cattgcgcca ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg
    21781 aacggcgatg gtttagaaat aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg
    21841 tttatgcgca gcgcgtgcgc ttacttatta caccgcagtc tggaaaatca ccccatcggc
    21901 tttttcggtg tactgagaaa gctggttgaa gttcagataa cgataagtat caacggcagt
    21961 tttatctact tgcgcaacgt aggtctggta ctcttccggc gtcggcagtt tgccaatcag
    22021 cgccgcaaca gccgccaatt ctgcagaagc caggaagaca ttcgcgccag tccccagacg
    22081 gttcgggaag ttacgggtag aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg
    22141 gttacccata cacagggaac agccagggat ctcgatacgc gcaccgctct taccgaagac
    22201 gctatagtag ccttcttcgg tcaactgcgc ggcatccata cgggttggcg gtgccaccca
    22261 caggcgcgtc ggcaactggc ctttgtgcgc atccagcagt ttacccgcag cacggaagtg
    22321 accgatgttg gtcatgcagg aaccgataaa cacttcgtcg atcttctcgc cctgtaccgc
    22381 agacagcgga cgtgcgtcgt ccgggtcgtt cggtgcacag aggattggct ctttaatatc
    22441 cgccagatcg atgtcgatca ctgccgcgta ttccgcatct gcatcggctt ccagcagctc
    22501 aggattcgcc agccattttt ccatgccctg aatacgacgt tccagggtac gacgatcgcc
    22561 gtaaccttcc gcgatcatcc acttcagcag gacgatgtta gagttcaggt attcgatgat
    22621 cggttctttg ttcagcttga tggtacaacc agcggcagaa cgctcggcgg acgcatcggt
    22681 cagttcaaat gcctgctcaa ctttcagatc cggcagacct tcaatttcca gaatgcgacc
    22741 agagaagatg tttttcttgc ctttcttctc aacggtcagc agaccttgtt tgatcgcata
    22801 cagcgggatc gcgtgtacca gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa
    22861 gcgcaccaga acggattccg gcatatccag cggcattacg cctgttgcgg ctgcaaatgc
    22921 caccagacca gaacccgccg ggaaagagat accgatcggg aaacgggtat gggagtcacc
    22981 accggtaccg acggtatccg gcagcagcat acggttcagc caggagtgga ttacgccgtc
    23041 acccggacgc agcgacacac caccacggtt cataatgaag tccggcagcg tgtggtgcgt
    23101 gttcacgtca actggcttcg gatacgccgc ggtatggcag aaagactgca tcaccaggtc
    23161 agccgagaag cccaggcacg ccaggtcttt cagttcatca cgggtcatcg ggccggtggt
    23221 gtcctgtgaa cctacagaag tcattttcgg ttcgcagtac gcgcccggac gaataccttt
    23281 cacaccgcag gcacgaccta ccattttctg cgccagcgag aagccgcgat cgctctcagc
    23341 gacatctttc gcctgacgga acacatcact gtgcggcaga ccaagtgctt cacgcgcttt
    23401 ggtggtcagg ccacgcccga taatcagcgg gatacggccg ccagcacgca cttcatcaat
    23461 cagcacgtcg gttttcagtt cgaaggtcgc cagcagttcg ccggtttcgt ggttacgcac
    23521 ttcacctttg tacgggtaaa cgtcaatcac gtcgcccatg ttcaggttag agacgtcgac
    23581 ttcgattggc agtgcacccg cgtcttccat cgtgttaaag aagatgggtg caattttacc
    23641 gccgaggcac aaaccaccgc cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa
    23701 ccacagaacg gagttcgtgg cggatttacg cgatgaaccc gtacccacaa cgtcaccgac
    23761 gtacgccagc gggaaacctt tctgttgcag agcttcaatc tgtttgatcg ggccaacaac
    23821 acctggctgg tctggctcaa taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag
    23881 tgggatatcc gggcgcgacc acgcatccgg tgccggagaa aggtcatcgg tgttagtttc
    23941 gccagtgact ttgaagacag taacggtcag tttttcagcc agcgccgggc gattcaggaa
    24001 ccattcggca tccgcccagg actgcataac ctgcttcgca tattcgttgc ctgctttcgc
    24061 tttctcttct acgtcataga agttatcgaa catcagcagc gtgtgagaaa gtgctttggc
    24121 agcaataggt gccagtttgg catcatccag cgcgtcgatc agcgaatgaa tgttgtaacc
    24181 accctgcatg gtgcccagca gttcgatggc tttttccgga gtcagcagag gggatttggc
    24241 ttcgcctttc gcgatagcag ccaggaagcc tgctttgaca taggcggctt catcgacgcc
    24301 tgggggaaca cggttggtta acagatctaa caggaattct tcttcgcccg cgggcgggtt
    24361 tttcagcagc tctacaagtg cggccatttg gtttgcatcc aggggtttgg gcgcaatccc
    24421 ctcagcggca cgctcagcta cgtgcttacg gtattcttct agcacgacgg ttctcctcgc
    24481 tctcattgtc atagtgcggc aggcgattct cttcacgctc ctgtgagaca gcagtttgta
    24541 gggtaaatgc ccagtaccgc aacgggcaga atagcaggat tttgacgatg tgttaatctg
    24601 tttacaaaaa agcaacataa aaaagtggct gaatcgttaa ggatggtcta gagattgttt
    24661 ccctccgtaa aaacttcgtt aattcgcatg gtgataatca cctttctcaa cgaatcccaa
    24721 gattagtcaa aatttaaact accgcctctt tatactcgga ttcacagcac ctgcgggtgg
    24781 cagttcgccg accattgcga tttccttgag atccgaatta tgaaaatgac tttgccgttt
    24841 aaaccccatg tgctggcgct aatttgcagt gccgggcttt gtgccgcctc taccgggcta
    24901 tatataaaaa gccgcacagt ggaagcgcct gtggaaccgc aatcgacaca acagactgcg
    24961 cctgacatca ccgcagttac gcttcctgca acggtttccg caccgcccgt aacgcccgcc
    25021 gtcgtcaaat ccgcattcag cactgcacaa atcgatcaat gggttgcgcc tgtcgcgctg
    25081 taccccgatt ctctgctttc acaagtgtta atggcatcaa cctatccggc aaacgttgct
    25141 caagcagtgc aatggtcgca cgataatcca cttaaacaag gcgatgctgc tattcaggcg
    25201 gtatctgacc agccgtggga cgccagcgtt aaatcactgg tggcctttcc acaattgatg
    25261 gcattgatgg gcgaaaaccc gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag
    25321 ccgcaggacg tgatggactc ggtacagcga ttgcggcaac tggcgcaaca aaccggttcg
    25381 ctgaagtcat caaccgaaca aaaagttatt accacaacga agaaagctgt accggtaaaa
    25441 cagacagtca cggcacccgt cataccatcc aataccgttt taactgccag ccccgtcatt
    25501 acagagcctg caacaaccgt catttccatt gagcccgcca atcctgatgt ggtctatatt
    25561 cccaactaca acccaaccgt ggtttacggg aactgggcca atactgcgta tccgccggtt
    25621 tatctgccac caccagccgg agaaccgttt attgacagct ttgtgcgcgg attcggctat
    25681 agcatgggtg ttgctaccac gtacgcacta ttcagcagca tcgactggga tgacgacgat
    25741 catgaccatc atcatcatga cgatgatgat tatcatcacc acgatggcgg tcatcgtgac
    25801 ggtaatggct ggcaacacaa cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt
    25861 atcaccggtg agcatcttac tgataagaat atggcatggc ggcacaatcc aaactaccgt
    25921 aatggtgtgc cctatcatga tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac
    25981 ggcggaatga gcgccacgca gctacctgct ccaacacgcg acagccagcg tcaggcggca
    26041 gcaagtcagt ttcagcaacg aacacacgcc gcccccgtca ttacacgaga tacccaacgt
    26101 caggcagcgg cacagcggtt taatgaagct gaaaactatg ggagctatga cgacttccgc
    26161 gacttcagcc gtcgccaacc actgacccag caacaaaagg acgccgctcg tcagcgttat
    26221 cagtcggcct cgcctgagca gcgccaggca gtccgcgaga aaatgcagac taacccacag
    26281 atccagcagc gaagagacgc agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc
    26341 caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg tgataacgcc
    26401 cgccagcgtg ttcaatcagc atcacctgaa caacgtcagg tttttcggga aaaagttcag
    26461 gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa taacgagcaa
    26521 cggtcagcag tacgcgaacg tctctctgag cgcggagcaa ggcgacagga aaggtaaatt
    26581 gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg ttccggcaaa
    26641 cgaacaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca
    26701 cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga
    26761 tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc agctcgccgc
    26821 cgttggtacc gacaatcgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct
    26881 tggtcatacc gtctgcgcag tcggaagcga tagcacgacc agaagcagcc cacgggaagg
    26941 tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg
    27001 caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag tagtgtttct
    27061 taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg
    27121 gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt
    27181 caacgcggat gaaaccacgg tcgtccactt ccacgccagc tttgcctgcg tcgaggtttt
    27241 taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagcgg
    27301 gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa
    27361 ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga
    27421 tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac
    27481 ccagcgcgtg gtatacggtg cccatttcca gaccgatgat accgccaccc attaccagca
    27541 ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt
    27601 catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcattgtcga
    27661 agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc ccggtgaatt
    27721 tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg
    27781 tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt
    27841 cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt
    27901 gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt
    27961 aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac
    28021 ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct
    28081 ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt
    28141 cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc gcttgctacc
    28201 ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt
    28261 tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat
    28321 ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt
    28381 gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga
    28441 tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa
    28501 tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact
    28561 tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac
    28621 ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct
    28681 taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt
    28741 tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac
    28801 cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacaac gaactgttga
    28861 agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga
    28921 tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca
    28981 gctcggtgat atcggttttg tcgaagtgag taacatgcgg gatcatcacc cagttacggc
    29041 tcaggttcgc accagaaatt ttctggatac ggcccagttc cacttcttcg atttcaccaa
    29101 acttgctgaa gtccaccttc ggccacggca gcatgcctgg gataccgccg ccagtcgccg
    29161 ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca
    29221 ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca aactcgcgtg
    29281 ccagacggcg gatcagcgga gtcgcgtgaa cgtaagcgtc gttttctgca aattcagatt
    29341 tgccttccgc tttcgcagcc ggtgctgctg ccggagcttc agcttttgct gccggagccg
    29401 gcgctgccgc ttcctgtttc gcaggagccg ccgcaggcgc tgcgccttca acttcgaaga
    29461 tcataatcag cgagccagtt ttcactttat cgccaacgtt gactttcagt tccttcacga
    29521 cgcctgcaaa cggagccgga acttccatag aagctttgtc gccttctacg gtaatcagtg
    29581 actgttcagc ggcaactttg tcgcccactt tcaccatcac ttcggtcact tcaacttcgt
    29641 caccgccgat atccggaacg ttaacttctt tcacgccagc cgctggtgca ggggccgctg
    29701 ccggagccgc ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga
    29761 agaccataat cagcgagccg gtagacactt tgtcacccac gttcactttg atctctttca
    29821 cggtgccagc aaacggagcc ggaacttcca tagacgcttt atcgccttct acggtgatca
    29881 gcgactgttc agcttcaact ttatcgccaa ctttcaccag gatttcggtc acttcaactt
    29941 cgtcgctgcc gatatccgga acgttaacgt cttttgccgc tgcagccgct ggtgctgctg
    30001 ccggagctgc ttctttcttc tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg
    30061 cggaatcgaa aatcataatc agtgcgccgg tctgggtttt atcgccaaca gagactttga
    30121 tctctttaac gatacccgcc tgcggagacg gaacttccat agaggctttg tcgccttcta
    30181 cggtgatcag cgactgttcg gcttcaactt tgtcgcccac tttgaccagg atctcggtga
    30241 tttcaacttc atcagccccg atgtccggta ctttgatttc gatagccatt attcttttac
    30301 ctcttacgcc agacgcgggt taactttatc tgcatcgatg ttgaatttgg cgattgcgtc
    30361 agcaaccact ttcttatcga tttcgccacg tttagccagt tcgcccagcg ccgctacaac
    30421 cacgtaggaa gcatcaactt cgaagtggtg acgcaggttc tcacggctgt cggaacgacc
    30481 gaagccatca gtacccagta cgcggtagtc gtcagccggt acgtaagtac ggacctgctc
    30541 agcgaacagt ttcatatagt cggtagatgc cactgccgga gcgtcgttca tcacctgagc
    30601 gatatacgga acgcgcggag tttccagcgg gtgcagcatg ttccagcgtt cacaatcctg
    30661 accatctcgc gccagttcgg tgaaggaggt cacgctataa acgtcagaac ctacgccgta
    30721 atctttcgcc aggatctctg ccgcttcacg gacgtgacgc aggatagaac cggagcccag
    30781 cagctgaact ttacctttgc taccttcaat ggtttcgagt ttgtagatac ctttacggat
    30841 accttcctca gcaccttccg gcattgccgg catgtggtag ttttcgttca gcgtggtgat
    30901 gtagtagtaa acgttctctt gtttttcacc gtacatacgc tccagaccgt catgcataat
    30961 gacagcaact tcgtaagcgt aagccgggtc gtaagagata cagttcggaa tagtcagcga
    31021 ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttcg ccgttcaggg tggtacgacc
    31081 ggaagtaccg ccgatcagga agccacgcgc ttgctggtcg ccagccgccc agcacagatc
    31141 gccaatacgc tggaagccga acatcgagta atagatgtag aacgggatca tcggcagatt
    31201 gttggtgctg taagaggtcg ccgctgccag ccaggaacaa cctgcgccca gctcgttgat
    31261 cccttcctgc agaatctgac ctttctcgtc ttctttatag taagcaacct gctcgcggtc
    31321 ctgcggggtg tactgctgac cgttcgggct gtaaatacca atctgacgga acagaccttc
    31381 cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc agacgatctt tgatcgactt
    31441 gttcttcagc attacgttca gggcacgaac gaaagcgata gtggtagaga tctctttgct
    31501 ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc agctcaagct tctcggtgaa
    31561 gttcggctga cggcttggca gataaccgtg cagtttctga cgctgagcgt gcagataggt
    31621 atgctcttca gaaccttccg ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga
    31681 caccggcaca ttgaaacggt cgcggatgtg acgcacgccg tccatgttca ttttcttaac
    31741 ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg ccgtaacctt taatggtatg
    31801 agcaaggatt actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat
    31861 tttcttcgga tcgtgaccac cacggttcag tgcccagatc tgctcgtcag tccagtctgc
    31921 aaccagtgct gcggtttcag gatatttacc gaagaagtgt tcacgaacgt acgcaccatc
    31981 tttcgatttg aaggtctggt agtcgccgtc aacggtttcg ttcatcagct ggatcagttt
    32041 accgctggta tctttacgca gcagttcatc ccaacggcta ccccacatca ctttgatcac
    32101 gttccagcca gcaccttcga agatgccttc cagttcgttg atgatcttgc cgttaccggt
    32161 gaccgggccg tcaagacgct gcaggttaca gttgataacg aagactaggt tatccagttt
    32221 ttcacgggta gcgatggtga tcgcaccttt ggattccggt tcgtccattt caccgtcgcc
    32281 gaggaacgcg taaacggtct gtttagaggt atctttcagg ccacggtgtt ccagatattt
    32341 caggaattta gcctggtaaa tagcaccaat cggccccaga cccatagata cggtcgggaa
    32401 ctgccagaat tccggcatca gtttcgggtg cggataggaa gagaggccat tgccgtgaac
    32461 ttcctgacgg aagttatcca gctgctcctg agtcagacga ccttctagga aagcacgagc
    32521 gtacacgccc ggggagatgt ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt
    32581 gcgtgcacgg aagaagtggt taaagcacac atcataaatg gttgcggaag actggaagga
    32641 cgccatatgg ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc
    32701 gttccagcgg atagctgaac gaatacggcg ttccagttcc agattacccg gatactccgg
    32761 ttgttcttca acggggatgg tgttgatgta gttgctgata cctgtgcctg cggctacgtt
    32821 tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc agatactgag cacgctcaac
    32881 accttcttca cggatgaccg attcgatcgc ctggagccag tcgcgagttt cgatcggatc
    32941 cacgtcattt gggaaacgtt ctgacatggg ttattcctta tctatctaat aacgttgagt
    33001 tttctggaac ctgtcccatt gaactctcgc cggaaagctc aataagacag gttctacgtt
    33061 tagttgccgc gcttttatat gcgcttgatt tacaacatct tctggataat ttttaccaga
    33121 aaaatcacta attctttcgt tgctccagac gacgcagaga acgctcacgg cggctctctt
    33181 cacgacttct gtcgagcaaa atttcttcga taaaggccag atggcgatgc gatgcttcgc
    33241 gcgcttcttc cggcttaccg gccataatcg cttcaaatat gcgggtgcgg tgactactca
    33301 ccagcggcag catctcgcga cgcgaataga gcaattcgaa gttctggcgg acattctggg
    33361 ccaacatcgg ctccatacac cttagcagat gaagcagaac cacattgtgg gccgcttcag
    33421 tgacagcaat ctgatactgg agtacggcgt ttgattccgc gtccagatcg ccagactgct
    33481 gcgccagctc tatggcgtgg tggagttcac ggatgcgttc cttgtcttca tcggtactac
    33541 gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat
    33601 actgtgactc aggatggtcg gagagcagct ccaccagcgg atcgctgaag ctttgccata
    33661 ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag caacaagccc ttcgcttcga
    33721 gacgttgaat cgcctcacgc aaggagggac gggagacgtc aaactgtttt gccagttcgc
    33781 gttccggtgg gagtttttcg cccgggcgga gagtgccttc gaggatcaaa aactccagtt
    33841 gctgctcaat cacatcggag agttttggtt ggcggatttt gctgtaggcc atgagttcct
    33901 gtcttaagcc acttgccgaa gtcaattggt cttaccaatt tcatgtctgt gacgctaaag
    33961 taacaaagta ttcaccttat gtccatacag gttttgattg aaatcatgaa actgtgcaca
    34021 ttttaacaac
//
LOCUS       DAZFRJ010000031        32630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000031 DAZFRJ010000000
VERSION     DAZFRJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.031"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..177
                     /locus_tag="ACHKUN_001349"
     CDS             <1..177
                     /locus_tag="ACHKUN_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6659645.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
     gene            221..328
                     /locus_tag="ACHKUN_001350"
                     /pseudo
     CDS             221..328
                     /locus_tag="ACHKUN_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHKUN_001351"
     CDS             complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHKUN_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO6659646.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1169..1789
                     /gene="fklB"
                     /locus_tag="ACHKUN_001352"
     CDS             1169..1789
                     /gene="fklB"
                     /locus_tag="ACHKUN_001352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6659647.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2098..3501
                     /gene="cycA"
                     /locus_tag="ACHKUN_001353"
                     /gene_synonym="dagA"
     CDS             2098..3501
                     /gene="cycA"
                     /locus_tag="ACHKUN_001353"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO6659648.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3543..3641)
                     /locus_tag="ACHKUN_001354"
                     /pseudo
     CDS             complement(3543..3641)
                     /locus_tag="ACHKUN_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3768..4196
                     /locus_tag="ACHKUN_001355"
                     /pseudo
     CDS             3768..4196
                     /locus_tag="ACHKUN_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4301..5368
                     /locus_tag="ACHKUN_001356"
     CDS             4301..5368
                     /locus_tag="ACHKUN_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659649.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHKUN_001357"
     CDS             complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHKUN_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO6659650.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHKUN_001358"
     CDS             complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHKUN_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6659651.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHKUN_001359"
     CDS             complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHKUN_001359"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6659652.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHKUN_001360"
     CDS             8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHKUN_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO6659653.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHKUN_001361"
     CDS             complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHKUN_001361"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO6659654.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHKUN_001362"
     CDS             11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHKUN_001362"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO6659655.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHKUN_001363"
     CDS             complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHKUN_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6659656.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHKUN_001364"
     CDS             12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHKUN_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO6659657.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHKUN_001365"
     CDS             complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHKUN_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO6659658.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHKUN_001366"
     CDS             complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHKUN_001366"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO6659659.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15439..17172
                     /gene="tamA"
                     /locus_tag="ACHKUN_001367"
     CDS             15439..17172
                     /gene="tamA"
                     /locus_tag="ACHKUN_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO6659660.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17169..20948
                     /gene="tamB"
                     /locus_tag="ACHKUN_001368"
     CDS             17169..20948
                     /gene="tamB"
                     /locus_tag="ACHKUN_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO6659661.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHKUN_001369"
     CDS             20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHKUN_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO6659662.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21504..21755
                     /gene="chpS"
                     /locus_tag="ACHKUN_001370"
     CDS             21504..21755
                     /gene="chpS"
                     /locus_tag="ACHKUN_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO6659663.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21749..22099
                     /gene="chpB"
                     /locus_tag="ACHKUN_001371"
     CDS             21749..22099
                     /gene="chpB"
                     /locus_tag="ACHKUN_001371"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO6659664.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHKUN_001372"
     CDS             complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHKUN_001372"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO6659665.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHKUN_001373"
     CDS             23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHKUN_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO6659666.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHKUN_001374"
     CDS             24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHKUN_001374"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO6659667.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHKUN_001375"
     CDS             25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHKUN_001375"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO6659668.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHKUN_001376"
     CDS             26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHKUN_001376"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO6659669.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHKUN_001377"
     CDS             complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHKUN_001377"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO6659670.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28842..30215
                     /gene="mpl"
                     /locus_tag="ACHKUN_001378"
     CDS             28842..30215
                     /gene="mpl"
                     /locus_tag="ACHKUN_001378"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO6659671.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHKUN_001379"
     CDS             complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHKUN_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO6659672.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHKUN_001380"
     CDS             31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHKUN_001380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO6659673.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32551..>32630
                     /locus_tag="ACHKUN_001381"
     CDS             32551..>32630
                     /locus_tag="ACHKUN_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6659674.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         7729 a         8134 c         8909 g         7858 t
ORIGIN      
        1 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
       61 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      121 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      181 aagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcccc
      241 ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
      301 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
      361 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
      421 attttcacca tttgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
      481 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
      541 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
      601 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
      661 gcgaccggag ccacctgatc aggatcattt tgtggggtga ctaattgcgc ccgcagctgt
      721 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccacc
      781 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
      841 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
      901 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
      961 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     1021 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     1081 ttgtcatcat tcctgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     1141 acttttatac cctaagaaag gaaatacgat gaccacccca acttttgaca ccatcgaagc
     1201 gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat ctggcctgga
     1261 agggctgctg ccagaagcac tggttgcagg tattgccgat gcgctggaag gcaaacatcc
     1321 ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa atccacgagc gcgccgatgc
     1381 cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc tggaagaaaa
     1441 cgccaaaaaa gaaggtgtga atagcaccga atctggcctg caattccgcg tgatcaacca
     1501 gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca ccggtaaact
     1561 gatcgacggc accgtgtttg acagctccgt tgctcgtggt gaacccgctg aatttccggt
     1621 taatggcgtg atccctggct ggattgaagc actgactctg atgccggtag gttctaaatg
     1681 ggaactgact atcccgcagg aactggcata tggcgagcgc ggcgcaggcg catccatccc
     1741 tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag cagcgcattc
     1801 tgttcccctc gaaccagagg ggagcaggca ttcagcaata aacccttcag tttgccaaac
     1861 ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct gtttcgcgtt
     1921 atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta aatcttagta
     1981 ttcatcccgc gtattgttac ctaatatcga tgagtcccga tacagattcg tcgtatcata
     2041 ggctgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga aaaaaacatg
     2101 gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc gctacggcgc
     2161 aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg tacggggttg
     2221 tttatggggt ctggcaaaac gattagcctt gccgggccgt cgatcatttt cgtttatatg
     2281 atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct gctttcgaat
     2341 ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg ggcaggatat
     2401 ttcaccggct ggacttactg gttctgctgg gttgtaaccg gtatggcaga cgtggtggcg
     2461 atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc ctcgctggcg
     2521 gtgatagtgc tgctgctgac gctcaatctc gccaccgtga aaatgttcgg tgagatggag
     2581 ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgattgtcgt cggcctggtt
     2641 atggtggcga tgcactttca gtcaccgact ggtgtggaag cgtcattcgc gcatttgtgg
     2701 aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt ccagatagcg
     2761 gttttcgctt tcgtagggat tgagctggta ggtacaaccg ctgcggaaac caaagatccg
     2821 gagaaatcac tgccgcgtgc aattaactct attccgatcc gtatcattat gttctacgtc
     2881 ttcgcgctga ttgtaattat gtccgtgacg ccgtggagtt cggtagtccc ggagaaaagc
     2941 ccgtttgttg aactgttcgt gttggtgggt ttgcctgcgg ctgccagcgt gatcaacttt
     3001 gtggtgctga cctctgcggc gtcttccgct aacagcggcg tcttctctac cagccgtatg
     3061 ctgtttggcc tggcccagga aggtgttgca ccgaaagcgt tcgctaaact ctctaagcgc
     3121 gcagtacccg cgaaagggct gaccttctcc tgcatctgtc tgctcggcgg cgtggtgatg
     3181 ctgtatgtga atcccagcgt gattggcgcg ttcacgatga tcacgacggt ttccgcgatt
     3241 ctgtttatgt tcgtctggac gattatcctt tgctcgtacc tggtgtatcg caaacagcgt
     3301 cctcatctgc atgagaagtc gatctacaag atgccgctcg gcaagctgat gtgctgggta
     3361 tgtatggcgt tctttgtgtt cgtgatcgtg ctgctgacac tggaagatga cactcgccag
     3421 gcgctgctgg ttaccccgct gtggtttatc gcgctggggt tgggctggct gtttattggt
     3481 aaaaaacgca tggcgaagta atgccgctaa ggcacctgcg ggtgtctttc aggctgcaaa
     3541 attgcctgat gcgctacgct tatcaggcct acagaacctc tgcaatatat tgagtttgcg
     3601 tgcttttgta ggccgggtaa ggcgtttacg ccgcatccgg catgaacaaa gcacactttg
     3661 tcagcaatct cctttgtctt tatacattcc taaaaatact tcccgtcatc gacagctctc
     3721 aggcgcgggc ttaccgtgga tctttgccac tcattgcaga gttcactatg gctaagcaca
     3781 gttggttatt aatcgccgca ttacctctct ccatttcacc ttcctggggc gcagattttt
     3841 attaccgcca gcaggagaaa ggcacggttt atgttgccga acagaaggga gaaaaggatg
     3901 agatcctctc cgaactgccg ggcgttaatt tttcccgcct ctggcgtatt gccaatttag
     3961 ccaataacca agaaacccgc ttactgtccg attttaatcc cgataagttc gattgcgatg
     4021 atcggaataa ttgccagcat acctggctca ccgatggacg ctctgttctt tggtctggca
     4081 aagtcctgaa aaatccgccc ggtgaaccta atgtcgatgc cgccagtttt caggcattcg
     4141 gtgctttcgc tgccgataaa cgcagcgtct attttgacgg tcagcgtacc gatgatagat
     4201 gaagtggata tgtatatagc tgtgtctcag ttgcaaacaa atatttatgt accgaaataa
     4261 aagtatcaat agacaattaa ttaataaatc aggagataaa gtggctatat tacatccaca
     4321 ggagtgctgg ttgctggaaa gaataatgtc gccggaatat tatcggcgtc gttttgaggg
     4381 ctggcaggag tttgtcgagc tgtgcgagcg acaggtggca gagtggagca agaccatgcc
     4441 gctggatgtg cgccgtcgtc cgctgtgcga gcagattgac gtcgtctggg gcgggcgagt
     4501 gttgcccaat atccgcagta ctttaaagtc ggtacagtat gactttattc agctacagca
     4561 gggggacctg cgggtactgc aatccggggg caatattagc agtgatatga aaggactaat
     4621 tgattatccg tcggactgga tgtcactcgc cgcgcaaaaa cagtatgaca gactgaaatg
     4681 gcgaggtgca cattataata atttaatcag acgtacctcg ggcggatact ggtatgacgg
     4741 cgaactgact tattattatg aggaaagtct gcacggcccg ctggccctgc cgatgcaatt
     4801 accgttgtat gagctggata gtagcgtata cctgcgtgag gatgactcgg taacggtggc
     4861 tggcctgtat ctgccggata tcccagatgc cagcgcccaa ctgttgtacc ggagtgaaca
     4921 tattcccgag gcttggcagg ggcgggtacg aacgaagtat gtcaacgagg ctgggataca
     4981 agaatattac tgggaaagcg gggcgtgggc aaaatgcaat tggaaacgta tacggcgggt
     5041 ggcaaaccga tttatcaatg tgccgccgga gggatttttc ccacagggca tgccggagga
     5101 gttgtacaac tggccacagc gcgaagcgca gtatgttaca gaccggcagc ggatagcggc
     5161 ctttagcggt gaagcttgtc ctcatagcgg ggagtggtcg atttttgtag aaggtagaca
     5221 agcgacggtg acactggagc agggagagca gatgccagaa tggacggaca ggaaaatgga
     5281 gggggagtat aaaagaggcg agaaattcca tgtgctgtgg tcgctgatga accgccatga
     5341 tggcgggtcg gtgtgggtgg aagcgtagcg gtagctggat gtacacgtaa aaaccaggga
     5401 atgggtgatt ttattctggc ttatacagga gcgatgaata agtaattata ttaatgaaaa
     5461 aaataagtta gtaaccgttg aaggaactta ttattggctg aataatgaat tttcccagtt
     5521 cagtagatac aaaagattgt cccggagtgg tgaataactc aaagcatttg aaaacgcacc
     5581 tgattgtgta aaccaggtgc gttgatcgaa caaattactc acccgccagc gcgcgtggga
     5641 acagtacatt gttttccagg ctgatgtgat ccatcaggtc atcaatcagt tcattaatac
     5701 cgttatacat cgctttccag gtggtgcagg cttccggcgg cggtgtgacg ttattggtgg
     5761 tgtgtttaat cacttccagc agttcgcccg cttcatcgtg ctcgctttcc atcacgctga
     5821 ttggtcccat tgcctggctg cccatgcctt gtttgatcat cgggaagagg atctgctctt
     5881 ctttcatcat gtggctggaa agctcttcat gcagcatagt caggtatttt gtcagccctt
     5941 ttggcacgct cggtttgtcg gcgtgaacgc gctcgacttt agtcgcttgc aggatcagct
     6001 ccggcagctg ctcgcggtga cgatcgtggt agcgcacgat gatatggtca atgatttctg
     6061 ccagcggggc gctacgccag tctttctcaa tcggttgttc agcaagcttt gccagttcag
     6121 cttcaatgac ctcaacatcc agttctttac gtgccgccgc gcgcgccagc gtctgcttac
     6181 cgccacagca gtaatccata tcatatttac gaaacagagc cgaagcgcga gggatagaga
     6241 gcgccagttc acctaaaggt tggtcgcgat aagccatagc tgatacctca ttcttaataa
     6301 gataagatgt attttaaatg catctttaag gcaaaagcta taacccttac gtagcagagg
     6361 cttatttaac tcactgccag cgtgtcaact ttctgagaca gattcagtta aaggtttttc
     6421 tggttttgct ctgaccgcaa tgaccacgcc aatcactaac agcgcgatac cgctcagcgt
     6481 cattagcggc ggcatttgct ggcggagtaa aaaggtgtac agcaaacctg ccagcgtttc
     6541 gaaaacaatc agcggcccga gaatcactgt cggtaatcgc tggctggcga cgttccagca
     6601 gagtgcgcca acccatgagc aaagcacggc tatcgcaacc atcagactaa taaacaccag
     6661 cggacggggg ccaaaaggta aggagaagtc cggcgtttgt atattcagcc agtaacaggc
     6721 gacgagatag ccgatgagag aaaccggcag cgtgaccagc gcctgcgccg tcgcccacat
     6781 catcggatgt ttgtcgggat tttcccgcag ccagcgggcg ttgcgcaggg cataccatgc
     6841 ccagcagacc acggaaacta acgctagcac gatgccagag gtataacgtg cccagtcaaa
     6901 atcggggagt ccgtggttta actcagcaat attcacactc gccaggccga tgccaataca
     6961 aatcagtgcc ggggcgagtt ttccccacgc gagtttgccg tcgcgctggc tataaagcag
     7021 attggcaaag acaggaatga ccaccggcag ggtgccgata atcatcgtgg aaacaggcgc
     7081 gccagtacgt tgaatggcac tggcaaggca gaagtaataa atgaggttgc ccatcatagt
     7141 gagcatcaag gcggtaagcc agtcccgacg cgccaactga cgcagacgca cgcgtcccag
     7201 ccaggcaatg ggcagcgcaa ttaaccctaa cgccagataa cgccccatcg actgcaacat
     7261 cgccgggtat tccggtacga tcaacggccc gacaaaaata agcccccaca tcaaccctgc
     7321 taacagggcg tacagcacgc cgctaatcat tactggcatc cattggtctg tcagaagaaa
     7381 gttcaggaag cagacatgat tgctcccggc atcccgtcat tattagagag gatgcgggag
     7441 gatgccactt taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac
     7501 gttgtcgttg ggcggccaat caatttgcta agcgttttgc tgttatcaaa cagaccacct
     7561 ttcgatgcgc caacgtcaga atccgccagc atatccgcca gtccgtcggg cagtccgacg
     7621 cttttcagtg ccgcggcgaa atcggcttcg ctcagatttt gataggtaac ctgtttgccg
     7681 ctctgtttgg tcagctctgc cgctaactgt gtcaatgtcc aggcactatc gcccgccagt
     7741 tcgtaaacct tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc tgccgcataa
     7801 tctgcccgcg ttgctgaggc gattttgcca tcgcccgccg caccgataaa tacgccgtgt
     7861 tccagtgctg ccggggcgct ggcgaggtag ttttcgctgt accagccgtt gcgcagcagg
     7921 gtgtaaacga tgccagaatc agccaacatt ttctccgtct cgatgtgctc atcggcgagg
     7981 ccgagcgggg aggtatctgc atgtagcagg ctggtataag cgataaattt cacaccagcc
     8041 gcctttgcgg cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa
     8101 gagatcagca gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg
     8161 tagtcagcct gacgcacggt aatgccttgt gctgccagag cctgggcttt tgccggatta
     8221 cgaacgatag ccactatttg gctgacagga accgttttct tcaaggattc aataacatag
     8281 tgaccaagtt ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc
     8341 ttgtgtttaa atacgctaac acctaaactt acttttagta agtacgcaca aaaaggtaag
     8401 tatgaaatga gtcaggttag cctgtcgcaa caactgaaag agggtaatct ctttgcggaa
     8461 cagtgcccgt cgcgcgaggt gttgaaacac gtcaccagcc gttggggggt gttgattctg
     8521 gtggcgctac gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat cggcggggtg
     8581 agcgaaaaga tgcttgcgca gtcgttacag gcgttagaac aggatggttt tcttaaccgt
     8641 atcgcgtatc cggtggtgcc gccgcatgtg gaatatagcc tcacgccgct gggcgagcag
     8701 gtgagcgaaa aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgttg
     8761 gcggtgcggg atgaacgtgc ggcataactg ccggatgcgc tgagcttatc tggtctacgg
     8821 tgcatggctt gtagggcgga taagatgcgc cagcatcgca tccgccaata aatgccggat
     8881 gcggttgatg atttataggc cgaaaaagaa gtgttttact tactcaaatc cacctgataa
     8941 atcgcaaacc cgatatcatc ggtcgcgact ttattcatcg gatattgccc tttctcttta
     9001 ataaacgctg cggctttatc tgacggagaa gtttcgaaac ggatatccag tttcttatcg
     9061 ccagctatcg gtgctaaacg ccagttgtta tctgccgccg ggtgaatttc ccccgcacgt
     9121 ttcgactcat cagcaatcca cgctgccagc accgagcggt tctcatccgg tgaagcaaaa
     9181 gcgatatggc tgtcgcccgt accggcaaat ttgccgccgt aagcgcgata gttattggtg
     9241 gcaacgagga acatggcgtt cggatcaatc ggcttgccat taaaggtcag gttcttaatc
     9301 ctttccgcat tggcattaat catctggcac tcgccgtcat aacgggcagg ctgggtaaca
     9361 tcaatctgat aattcacacc atcaatcaca tcaaagttat aggtgcggaa accatcccag
     9421 ttgatgagtg attgcggttt cgtgctgtta ggatcaatct ggttaaactg tccggcggag
     9481 cactccagcc actctttcac ctctttaccg ctggctttca ccacaatcag cgtattgggg
     9541 tagagataaa gatcggcggc attacggaag gtcaactggc ctttttccac ctccacatag
     9601 cttgccgggt catttttgcg accaccaact ttaaacggtg cggcagctga aagcaccggc
     9661 agttttgcca gatccggatc gccctgaatg taatgttcga catacgcttt ttgcgcgttg
     9721 ttcactactt gcacggtcgg atcgtcctgc accagcgcca gatagctata catattgtcg
     9781 gcagatttac cgattggctt gctgacgaac tggcgtgtgg catcgtgatc ggctttgagt
     9841 gtttctacca gcttgctgtc ttccgccgcg agggattttt tattggcgat gtcgtaaatc
     9901 ggccgagctt ccgctttcgc ctgcgtcacc tgccatttac cgctgtcatt actgagttgt
     9961 aagtcgacca ccccaagatg atcgccccac atgcccggca ttaccgccgg aacaccattc
    10021 agcgtgcctt tggtgatatc agccccttcg atatcagcaa aatctttacc cgggaaaacg
    10081 gcgtgagcat gaccaaacat aatggcgtta acgcccggaa tttcactgag gtaataaact
    10141 gagttttccg ccattacttt atacggatcg gcagacagcc cggaatgcgc cagaacgaca
    10201 acgacatcgg cacctttctc gcgcatttca ggcacgtatt tgcgcacggt ttcggtaata
    10261 tcattcaccg tcacttttcc ggataaatta gctttatccc agcccatgat ttgcggcggc
    10321 acgacgccaa tatagccaat cttcagcgtc tgtttttttc cgtctttatc gaccacttcg
    10381 gtatctttaa ttaaatacgg tgtaaacatt ggctgtttgg ttctggcgtc aatgacgttg
    10441 gcatttacat aagggaattt cgctcccgcc agcgcatttt tcaggtaatc cagaccgtag
    10501 ttaaattcat gattgccgag tgtaccgacc gtataatcca gcgtattcag cgccttataa
    10561 accggatgaa catcacctgc ttttaatcct ttcgccgaca tgtaatcggc cagcggactc
    10621 ccctgaatca aatcgccgtt atcgaccagt acgctgtttt tcacttcatt gcgggcatcg
    10681 ttaatcaggc ttgccgtacg taccagtccg aatttttccg tggcggtgtc tttgtaataa
    10741 tcgaaatcca tcatgttgct atgcagatca gtggtttcca tgatacgcag atcgaccgtc
    10801 gctgcattca cactggcggc aatcagcgtg gccaggagcg ttgcgctaaa cttaatcatc
    10861 agggacatcc ttttatcatc gggaatacga aagaaaaggg agaataaacg tcttacttat
    10921 agaacagtga agaatgccac aattttacgc tttgaaaatg atgacactat cacagttggc
    10981 gcattcatta acgatagggt ataagtaaaa caataagtta acaccgctca cagagacgag
    11041 gtggagaaat gttagatcaa gtatgccagc ttgcacggaa tgcaggcgat gccattatgc
    11101 aggtctacga cgggacgaaa ccgatggacg tcgtcagcaa agcggacaat tctccggtaa
    11161 cggcagcgga tattgccgct cacaccgtta tcatggacgg tttacgtacg ctgacaccgg
    11221 atattccggt cctttctgaa gaagatcctc ccggttggga agtccgtcag cactggcagc
    11281 gttactggct ggtagacccg ctggatggta ctaaagagtt tattaaacgc aatggcgaat
    11341 tcaccgttaa cattgcgctc attgaccatg gcaaaccgat tttaggtgtg gtgtatgcgc
    11401 cggtaatgaa cgtaatgtac agcgcggcag aaggcaaggc ctggaaagaa gagtgcggtg
    11461 tgcgcaagca gattcaggtc cgcgatgcgc gcccgccgct ggtggttatc agccgttccc
    11521 atgcagatgc ggagctgaaa gagtatctgc aacagcttgg cgaacatcag accacgtcca
    11581 tcggctcttc gctgaaattc tgcctggtgg cggaaggaca ggcgcagctg tacccgcgct
    11641 tcggaccaac gaatatttgg gacaccgccg ctggacatgc tgtggccgca gctgccggag
    11701 cgcacgttca cgactggcag ggtaaaccgc tggattacac tccgcgcgag tcgttcctga
    11761 atcctgggtt cagagtgtct atttattaat taatttatgc agcaggtcca tcacttgctg
    11821 cacctcttcc tgagtaagcg ccccgtcttt ggcccattgc acgcgaccgt ctttatccag
    11881 caccactaca gcggaacttt tttcatccag ctgccaggca ccgcgtgcga cgccattgct
    11941 atcaacaata aactgcgacc agggataaag ctttttatta ctctccagac tgctgcgcac
    12001 aaacatgccg gaacccggaa ttgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg
    12061 atcgtgcggt aacttcgctg atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc
    12121 agaggtgcga ccagcaatat gttgcagtac tcgcactttt cccactaact gcgcgctgtt
    12181 ccaggttttg tagctaaact gatctttatc aagcaccaac tcgccacgat cggtgatgcc
    12241 aatcggcggc actcgctgac cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag
    12301 caggcaggtg agtgccagaa tcttgcgtag ggtcatggtg tttccttctt atgatatgca
    12361 ggtgatccga ccacttgggt ctgagtttta atcatatgtg ctatttatcg aatttcccgc
    12421 aagtgtgatg ccagtttgcg gtcaagcgca caaatcatat gaaaaatgaa tgcttatact
    12481 gaagaccgcg cttcggtaaa aagataattc tgaataattg taacctttag gtaaaaaaag
    12541 ttatacgcgg tggaaacatt gcccggatag tctatagtca ctaggcatta aaatttgcgc
    12601 ctcataatag ttgggccgat tgtggcaccg cacaggcgta atactcagca ggagataaca
    12661 atgaaaattt tccaacggta caacccactt caggtggcga agtacgtaaa gatcctgttc
    12721 cgtggacggt tatacatcaa ggacgttggc gcttttgaat ttgataaggg taagattctt
    12781 atcccaaaag tgaaggataa gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt
    12841 atgcgtctgc aaacagagat ggcttaacca aagtgctatg cagtaataag acggctcctg
    12901 attcaggagc cgttgatgtt tctgggggtt acgcgacgct ttcttcttta tctttagcgt
    12961 caggcagttt tggcgaaagg gcggtggcct tgctgtcgat ccgggtcacc agcagctggt
    13021 cgatgcggta gttatcgata tccaccactt caaatttgta gccggcgaat ttcaccgaat
    13081 cggtgcgttt cgggatctta cgcagcataa acatcataaa gcctccgatg gtttcgtagt
    13141 tgcccgactg cgggaactcg tcaatatcca gcacgcgcat gacgtcgtca attggggtgc
    13201 cgccgtcaat cagccatgag ttctcatcac gggcgacaat ctgctcttcc agcccctgac
    13261 cgaccagatc gcccatcagc gtggtcatca cgtcgttgag ggtgatgatc cccaccacca
    13321 gcgcgtactc gttcatgatc accgcgaagt cttcacctgc ggttttaaaa ctttccaacg
    13381 cctctgaaag ggttaacgta tccggcacaa tcagcgtgtt gcgaatttgt acgccgctgt
    13441 tcagtgccag gctttggtta gccagcacgc ggttcagcag gtctttagaa tcgacataac
    13501 cgatgatgtg gtcaatatct tcattacaga cgaggaactt agagtgcgga tgttccgcca
    13561 ccttattctt caggctttgc tcatcttcgt ggagatcaaa ccaaatcacg ttttcacgcg
    13621 gtgtcattga agacggaacg gtacgggatt ccagctcaaa gacgttttca atcagctcgt
    13681 gttcctgttt acgtaacacg cccgccagcg caccggcttc cactaccgcg tagatgtcat
    13741 cagaagtgat gtcatcttta cgtaccattg gcagtttgaa aatacggaag atcatgttcg
    13801 ccaggccgtt gaagaaccac accagcgggg tgcaaacgta caggcagaag cgcatcgggt
    13861 tgatgatacg caaagccacc gcttctggcg caatcatacc gatgcgtttc ggggttaaat
    13921 ccgcaaacag gataaacatg ccagtcacta acgagaaaga gagaataaag ctcagttgct
    13981 cagagagttc tgccgacata tagcgggaga acaggctgtg aaaagctgga gaaaatgccg
    14041 catcaccgac gataccgccg agaatcgcaa ctgcgttcag accgatttgg accaccgtaa
    14101 agaacatgcc gggattttcc tgcatattca gaacgcgttg ggcatttata ttgccttcat
    14161 cagccagcag tttaagtttg attttgcgtg aggcggcaag tgagatctca gacatcgaga
    14221 agaacgcact tacagcgatc aagcagagta tgactaaaat actgtttaac atatcttatc
    14281 cgacctttca ggccggatcc tcggaagggg aagttgatta atttgtgtga aatacacatt
    14341 gaaagccgat tcgaagaaag tgaaccggca ttttcagtgg ctagtatagc gtaaggtact
    14401 gtaaagtcgc cagagggtta aaattcagac tgctgataaa tgcaatactg cctgatgcgc
    14461 tatgccgatc aggcctacgc agtccctgca atatattgaa tttggacgat tttgtaggcc
    14521 tggtaaggca ttcacgccgc attcggcata aacaaaacgc actttatcaa caatctacat
    14581 ttgtacccgt aacgctatgc ttccggtggc agacagacgc caattccgcc aattccacag
    14641 taaccatacg ggtttttatg cagatattgc tggtggtcat cttcggcata ataaaacggt
    14701 gtggcgttag cgatttccgt ggtgatgtga cgatcgtcat cggcggcaag catcgccgcc
    14761 tgaaaacgtt ccagactggc gcgagctgcg gcatcctgtt ctggggtcag cggataaatc
    14821 gctgaacgat actgcgtgcc gtggtcattg ccctgacgca ttccctgggc gggatcgtga
    14881 ttctcccaaa atacctgtag caactgctca tagctgatga cagaaggatc gtaaacaatg
    14941 cgtaccgctt cggcatgacc cgtatcaccg gagcacactt cccgataagt cggatttggc
    15001 gtatagccgc cggtatagcc tgcggcggtg ctgtaaacgc cgggtaactg ccagaacaga
    15061 cgctccacac cccagaaaca acccatcgca aaaatggcaa tctccattcc gtcaggtaca
    15121 ttggtcattg agtgaccgtt gaccgcatgc agcgtggcta cgggcatcgg ggtgttacgt
    15181 ccaggcaggg catcggcggg ggaaaccaga tgctttttat caaataaact catggtgtcg
    15241 ctctcccgaa aatcggtcat tggggttaag gttgtaacaa gaggcgtatt tgcacacaat
    15301 aaccaccgtg aataggtcta aagtaaaaca taagaaatat ttggggttta gtctgctttt
    15361 taatccatat tactggattt ttgttaagcc gtttaacggc gttccagggg caggaaaaaa
    15421 ggatattcag gagaaaatgt gcgctatatc cgacagttat gctgtgtaag cttactctgc
    15481 ttaagcggat ctgccgtcgc cgcgaacgtc cgtctacagg tcgaggggtt atcgggacag
    15541 ctggaaaaga acgttcgtgc gcagctttct acgattgaaa gtgatgaagt gacgccagac
    15601 cgtcgctttc gcgcacgcgt cgatgatgcc atccgcgaag gtctgaaagc gctgggttat
    15661 taccagccga ccattgaatt tgatctccgt ccaccgccaa agaaagggcg gcaggtattg
    15721 atcgccaaag tcacgccagg cgtgccggtg ttaattggcg gcaccgatgt ggtattgcgc
    15781 ggcggcgcgc ggaccgataa agactatttg aaattgctcg atactcgccc ggctattggc
    15841 acggtactga accagggcga ttatgaaaat ttcaaaaagt ccttaaccag cattgcgttg
    15901 cgtaaaggtt atttcgatag cgaatttacc aaagcgcagc tgggcattgc gctcggcctg
    15961 cataaagcct tctgggatat tgattataac agtggcgaac gttaccgctt tgggcatgtg
    16021 acctttgaag gatcacaaat ccgcgatgaa tacctgcaaa atctggtgcc gtttaaagag
    16081 ggcgatgagt acgaatcgaa agatctggcg gaacttaacc gtcgactttc tgctaccggc
    16141 tggtttaact cggtcgtagt ggctccacaa tttgataaag cgcgcgaaac gaaagtatta
    16201 cccttgacgg gcgtggtttc gccgcgaact gaaaacacca tcgaaaccgg ggtcggttac
    16261 tctacggacg tgggaccgcg cgtgaaagct acgtggaaaa agccgtggat gaactcttat
    16321 ggtcacagtc tgaccaccag taccagtatt tccgcgccgg aacagaccct cgacttcagc
    16381 tataaaatgc cgctgctgaa gaatccactg gaacaatatt atttggtgca gggcggtttt
    16441 aagcgcactg acctgaacga taccgaatct gactccacta cgctggtggc ttctcgctac
    16501 tgggatctct ccagcggctg gcagcgtgcc attaacctgc gctggagtct cgaccacttt
    16561 actcagggtg aaattaccaa caccacgatg ctgttttatc ctggggtgat gattagccgc
    16621 acgcgttctc gtggtggcct gatgccaacc tggggcgact cgcaacgcta ctctatcgac
    16681 tactccaaca cggcctgggg ctcagatgtc gatttctccg ttttccaggc gcagaacgtc
    16741 tggatccgca cactgtacga tcgccatcgt ttcgttacac gcggcacgct gggctggatt
    16801 gaaaccggtg atttcgacaa agtaccgccg gatctgcgtt ttttcgccgg gggcgatcgc
    16861 agtattcgcg gctacaaata caaatctatc gccccgaaat acgccaacgg tgacctgaaa
    16921 ggggcctcga agttgataac cggatcgctg gaataccagt acaacgtgac cggaaaatgg
    16981 tggggcgcgg tgtttgtcga tagtggcgaa gcggtaagcg atattcgccg cagcgacttt
    17041 aaaaccggta ccggggtcgg cgtgcgctgg gaatcgccgg tcgggccaat caaactcgat
    17101 tttgccgtac cggtcgcgga taaagacgaa cacgggttac agttttacat cggtctgggg
    17161 ccagaattat gagtttatgg aaaaaaatca gcctcggcgt ggttatcgtt atcttactgt
    17221 tgctgggatc ggtggcgttt ctggtgggca ccaccagcgg cctgcatctt gtatttaaag
    17281 cggcggatcg ctgggtgcca ggactggata ttggcaaggt caccggcggc tggcgcgatc
    17341 tcaccttgtc tgacgttcgt tatgagcagc caggcgtggc ggtaaaagcg ggtaatctgc
    17401 atctggcggt cggtcttgag tgcctgtgga acagtagcgt ttgtattaat gacctggcgc
    17461 tgaaagacat tcaggtcaac atcgacagta aaaaaatgcc tccttctgaa caggttgaag
    17521 aagaggaaga tagcggtccg ctggatctct ccacgccgta tcccatcacc ctgacacggg
    17581 tggcgctgga caacgtcaac atcaagattg atgacaccac ggtgtcggtg atggacttca
    17641 cctccggcct gaactggcag gagaaaaccc tgaccctgaa accgacgtcg ctgaaaggcc
    17701 tgctgattgc tctgccgaaa gtggcggaag tggcgcagga agaagtggtc gaaccgaaaa
    17761 ttgaaaatcc gcagccggag gaaaagccgc tcggcgaaac gctgaaagat ctcttttctc
    17821 gcccggtatt gccggaaatg accgacttgc atttgccgct taacctgaac attgaagagt
    17881 ttaaaggcga gcagttacgc gtgacgggcg acacggacat caccgtgcgc accatgctgc
    17941 tgaaagtgag cagcattgac ggcaatacta aactggacgc cctggatatc gattccagtc
    18001 aagggatcgt caacgccagc ggcacggcgc agctgtcaga caactggccg gtggatatca
    18061 ccctcaacag cacactgaac gtggagccgt tgaaaggtga aaaagtgaag ctaaaagtgg
    18121 gcggcgcgct gcgcgaacag ctggagattg gcgttaatct ttccggtccg gtggatatgg
    18181 atttacgcgc ccagacgcga ctggcggaag ccggattgcc actcaacgtg gaagtgaaca
    18241 gcaaacagct ttactggccg ttcaccggtg agaagcagta tcaggcggat gatctgaaac
    18301 tgaaacttac cggcaaaatg accgattaca cgctctctat gcgtacggca gtgaagggac
    18361 tggagatccc gccagccacc attacccttg atgccaaagg taatgaacag caggtcaatc
    18421 tcgacaaact caccgtcgcg gcgctggaag ggaaaactga actcaaggcg ttgctcgact
    18481 ggcagcaggc cattagttgg cgcggtgagc taacgcttaa cggcattaac accgccaaag
    18541 agatcccaga gtggccgtcg aaactcaatg gcttgattaa aacccgcggt agcctgtacg
    18601 gcggcacctg gcagatggag gtgccagaac tgaagctgac cggtaacgtc aaacagaaca
    18661 aagtgaacgt tgacggcacg ctgaaaggca acagttatat gcagtggatg atcccagggc
    18721 ttcatctgga actggggcca aacagtgccg aagtgaaagg cgagctgggg gtaaaagatc
    18781 tcaatcttga tgccaccatc aacgcgccgg ggctggataa cgcgctgccg gggcttggcg
    18841 gtacagcgaa agggctggtg aaagttcgcg gcacggtgga agcgccacaa ctactggcgg
    18901 acatcaccgc gcgcggcctg cgctggcagg agctaaccgt ggcgcaggtt cgcgtggaag
    18961 gcgatatcaa atccaccgtt cagattgcgg gtaaactcga cgtgcgcgtt gagcaaattt
    19021 cacagccgga tgtaaatatc aacctcgtca ccctgaatgc caaaggcagc gaaaaacagc
    19081 acgagttaca gttgcggatt cagggcgagc cggtttccgg gcagcttaat ctggcaggaa
    19141 gttttgatcg caaagaagaa cgctggaagg gaactcttag caatacccgc ttccagacgc
    19201 cggtcggccc gtggtcgctg acccgcgata ttgcgctgga ttaccgcaat aaggagcaaa
    19261 aaatcagcat cgggccacac tgctggctta acccgaatgc ggaactgtgc gtgccgcaaa
    19321 ctatcgatgc gggggccgaa gggcgtgcgg tggtgaatct caaccgcttc gacctcgcca
    19381 tgctgaaacc gtttatgcca gaaaccactc aggccagcgg tatcttcacg ggtaaagcgg
    19441 atgttgcctg ggacaccacg aaagaggggc tgccgcaggg cagtatcacc ctttcggggc
    19501 gtaacgtgca ggtaacgcaa accgtcaacg atgcggcgct gcccgtggcg tttcagacac
    19561 tgaatctgac ggcggaattg cgtaacaacc gtgccgaatt gggctggacc atccgcctga
    19621 ccaataacgg ccagtttgat ggacaggtgc aggtgaccga tccgcaaggc cgccgtaatc
    19681 ttggtggcaa cgtcaatatc cgtaacttca accttgcgat gataaacccc atctttaacc
    19741 gtggggaaaa agcagcgggg atggtgagtg ccaacttgcg tctgggtggt gatgtgcaaa
    19801 gcccgcagtt gtttggtcag cttcaggtta cgggtgtgga tatcgacggc aactttatgc
    19861 cgtttgatat gcaaccgagc cagcttgcgg tcaactttaa tggtatgcgc tcgacgcttg
    19921 ccggtacagt acggacccag cagggtgaaa tctacctgaa cggtgatgcc gactggagcc
    19981 aaattgaaaa ctggcgggcg cgggtaacgg cgaaaggcag taaagtgcgg atcaccgtgc
    20041 cgccgatggt acgaatggat gtatcgccag atgttgtctt cgaggctaca ccaaacctgt
    20101 ttaccctcga tggtcgcgtg gatgtcccgt gggcgcgcat cgtggtgcac gatctgccgg
    20161 aaagcgcagt aggcgtctcc agcgatgtgg tgatgcttaa cgataacctg caacctgaag
    20221 agccgaaaac ggcgtcgatt ccgattaaca gtaacctgat tgtccacgtt ggcaacaatg
    20281 tgcgcattga cgcctttggc ctgaaagcgc ggctgacggg cgatcttaac gtcgttcagg
    20341 acaaacaagg gctgggcctg aacgggcaga tcaacatccc tgaagggcgc ttccatgcct
    20401 atggtcagga tctgattgtg cgtaaaggtg agttactgtt ctctggtccg ccggatcaac
    20461 cgtatcttaa tattgaagct attcgtaacc cggatgctac agaagacgac gtaatcgccg
    20521 gagttcgcgt cactggtctg gcggacgaac cgaaagcgga gatcttctct gacccggcga
    20581 tgtcgcaaca agctgcattg tcttatttgc tacgtggaca agggctggag agcgatcaga
    20641 gcgacagtgc ggcaatgacc tcgatgctga ttggtctggg ggttgcgcaa agtggccaga
    20701 ttgtgggtaa aatcggcgag acgtttggcg taagcaattt agcgctcgac acccagggag
    20761 taggcgactc ctcccaggta gtggtcagcg gctatgtatt gccaggtctg caagtgaaat
    20821 atggcgtggg tatatttgac tctatagcaa cactcacgtt acgttatcgc ctgatgccta
    20881 agctatatct ggaagccgtg tctggtgtag accaggcact ggatttgctc tatcagttcg
    20941 agttttagca atgcgaatat ttgtctacgg cagtttacgc cacaaacaag gcaacagtca
    21001 ctggatgacc aatgcccagt tactgggcga tttcagtatc gataactacc agttgtatag
    21061 cctgggccac tatccaggcg cagttccggg gaacggaaca gtacacggtg aagtttatcg
    21121 tattgacaac gccacgctgg ccgaacttga tgccttgcgc accaggggcg gtgaatacgc
    21181 gcgccagttg attcagacgc cgtacgggag tgcatggatg tacgtttatc aacgacccgt
    21241 cgatggatta aagctaattg aaagcggcga ctggttagac agggataagt aaccatatgc
    21301 atacgccacc ttcgggtggc gttgtttttt gcgagacgac tcgcattctg ttttgtaatt
    21361 ccctcaccct ttgcttttct ctccgagccg ctttccatat ctgttaacgc ataaaaaact
    21421 ctgctggcgt tcacaaatgc gcaggggtaa aacgtttcct gtagcaccgt gagttatact
    21481 ttgtataact taaggaggtg cagatgcgta ttaccataaa aagatggggg aacagtgcag
    21541 gtatggtcat tcccaatatc gtaatgaaag aacttaactt acagccgggg cagagcgtgg
    21601 aagcgcaggt gagcaacaac caactgattc tgacacccat ctccaggcgc tactcgcttg
    21661 atgaactgct ggcacagtgt gacatgaacg ccgcggaact tagcgagcag gatgtctggg
    21721 gtaaatccac ccctgcgggt gacgaaatat ggtaaagaaa agtgaatttg aacggggaga
    21781 cattgtgctg gttggctttg atccagcaag cggccatgaa cagcaaggtg ctggtcgacc
    21841 tgcgcttgtg ctctccgttc aagcctttaa tcaactggga atgacgctgg tggcccccat
    21901 tacgcagggc ggaaattttg cccgttatgc cggatttagc gttcctttac attgcgaaga
    21961 aggcgatgtg cacggcgtgg tgctggtgaa tcaggtgcgg atgatggatc tacgcgcccg
    22021 gctggcaaag cgtattggtc tggctgcgga tgaggtggtg gaagaggcgt tattacgctt
    22081 gcaggcggtg gtggaataag gtgtgtttat ttatcgcggg cataaaaaaa cccttactaa
    22141 ccgaagctcg gcgttcaggg ttattgcgct agaagaactt atttattctt tgcgcgctcg
    22201 aaggaagcaa cgatttcagc tttagcggct tctgcgtttt cccaaccttc aactttcacc
    22261 cacttgcctt tttcgaggtc tttgtagtgc tcgaagaagt gagcgatctg cgctttcagc
    22321 agctctggca gatcgttaac gtctttaatg tgatcgtatt ctttgctcag cttgctgtgc
    22381 ggaaccgcaa ccagtttcgc atcttcaccg gcttcgtcgg tcattttcag aacgccaacc
    22441 ggacggcaac ggatcacaga acccggctgc agcgggtacg gagtcgggac cagtacgtca
    22501 accgggtcac cgtccagaga cagggtgtgg ttgatgtaac cgtagttgca cggatagaac
    22561 atcgcggtgg acatgaagcg gtcaacgaac agtgcgccgc tctctttgtc gatttcgtat
    22621 ttgatcggat ctgcgttagc cgggatctca ataacaacgt agatgtcttc cggcagatct
    22681 ttacccgcag ggacgttgag taagctcatg tctgtttcct ttaaaaatat gtggcaaaca
    22741 agtgccgagt attatagcca actcgcgccg aatgtctttg cttgttttcg tcttcatttt
    22801 cctttttcgc cagttttaag acggtataca gagcaggaaa atccataacc gtagccgcat
    22861 ttttcatagt gagatgaaag cgattacaaa cttgtgatta acgttttatt tacttttttg
    22921 aagtgtgatg taacgcaatc cgttacataa cgaattgtct atagtttttt cgcgaacatc
    22981 ttttcaccaa taataattac cccgacgagg acaaccctat gtggaaacgc ttacttgtag
    23041 tctctgcagt ctcggcagcc atgtcgtcta tggcgttggc cgctccatta accgtaggat
    23101 tttcgcaggt cggatcggaa tccggctggc gcgctgcaga aaccaatgtg gcgaaaagtg
    23161 aggccgaaaa acgcggaatt acgttgaaaa ttgccgatgg tcagcaaaag caggaaaacc
    23221 agattaaagc ggtacgttcc ttcgttgcac aaggggtgga tgcgatcttt atcgctccag
    23281 tggttgcgac gggttgggaa ccggtattaa aagaggcgaa agatgccgaa atcccggtct
    23341 tcttgcttga ccgttccatc gatgtgaaag acaaatctct ctatatgacc accgtcactg
    23401 ccgacaacat cctcgaaggc aagttgattg gtgactggct ggtaaaagaa gtgaatggca
    23461 aaccatgcaa cgtggtggaa ctgcagggca ccgttggggc cagcgtcgcc attgaccgta
    23521 agaaaggctt tgccgaagcc attaagaatg cgccaaatat caaaatcatc cgctcgcagt
    23581 caggtgactt cacccgcagt aaaggcaaag aagttatgga gagctttatc aaagcggaaa
    23641 acaacggcaa aaacatctgc atggtttacg cccataacga tgacatggtg attggtgcaa
    23701 ttcaggcaat taaagaagcg ggcctgaaac cgggcaaaga tatcctcacg ggttccattg
    23761 acggcgtacc ggatatctat aaagcgatga ttgatggcga agcgaacgcc agcgttgaac
    23821 tgacgccgaa catggcaggc cccgcttttg acgcgctgga gaaatacaaa aaagacggca
    23881 ccatgcctga aaagctgacc ctgaccaaat ccacccttta tctgcctgat accgcaaaag
    23941 aagagttaga gaagaagaaa aatatggggt attgagggtt gctatgcctg atgccgattc
    24001 gtaggccgga taaggcgctc gcgccgcatc cggcgatggt gcactgaagc ctgatgcgac
    24061 gcttaccgcg tcttatcatg cctactggga gcacgcttta caccggggga aaccatgacg
    24121 accgaccaac accaggagat cctccgcacc gaaggattaa gtaaattttt ccccggcgtc
    24181 aaagcgttag acaacgttga tttcagcctg cgccgtggcg aaatcatggc gctgcttggt
    24241 gaaaacgggg cgggaaaatc aacgctaatc aaagcattaa ctggtgtata ccacgccgat
    24301 cgcggcacca tctggctgga aggccaggct atctcaccga aaaataccgc ccatgcacaa
    24361 caactcggta tcggcaccgt ctatcaggaa gtcaacctgc tacccaatat gtcggttgct
    24421 gataatctat ttataggccg cgaacccaaa cgtttcggcc ttctacgccg taaagagatg
    24481 gaaaagcgcg ccaccgaact gatggcatct tacggtttct ccctcgacgt gcgcgaaccg
    24541 cttaaccgtt tttcggtggc gatgcagcaa atcgtcgcca tttgccgggc tatcgatctc
    24601 tccgccaaag tgctgatcct cgatgaaccc accgccagcc tcgacaccca ggaagtggag
    24661 ctattatttg gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgat ctttgtcact
    24721 cactttctcg atcaggtcta tcaggtcagc gatcggatca ccgtcttacg caacggcagt
    24781 ttcgtaggct gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa aatgatgctg
    24841 gggcgcgagc tggataccca cgcgctacag cgtgccgggc gaacattgtt gagcgacaaa
    24901 cccgttgccg cgttcaaaaa ttacggcaaa aaaggaacga tagcaccgtt tgatctcgaa
    24961 gtgcgccccg gcgagatcgt cggtctggct ggcttgctgg gatccggacg taccgaaacc
    25021 gccgaagtga tcttcggtat caaacctgct gacagcggca cggcgttgat caaaggcaaa
    25081 ccgcaaaccc tgcgatcgcc acatcaggct tcagtactgg gcatcggatt ctgcccggaa
    25141 gacaggaaaa ccgatggcat catcgctgcc gcctcggtgc gggaaaatat catcctcgct
    25201 ctccaggccc agcgcggctg gctacgaccc atttcccgca aagagcagca agagattgcc
    25261 gaacgcttta tccgccagct tggcattcgc acaccttcaa ctgaacaacc gattgaattt
    25321 ctctctggcg gcaatcagca aaaagtgttg ctttcacgtt ggctactgac acgaccgcaa
    25381 tttctgatcc tcgacgagcc aacccgcggc attgatgttg gtgcccacgc cgagatcatc
    25441 cgcctgattg aaacgctgtg tgctgacggt ctggcgctgt tggtgatctc ctccgaactg
    25501 gaagagctgg tgggctatgc cgatcgggtg attatcatgc gcgatcgcaa acaggtggcg
    25561 gagatcccgc tggcagagct ttccgttccg gcgatcatga acgccattgc ggcgtaagga
    25621 gaacagtgtg atgcctcaat ctctcccgga cactacgccg ccgaaaaggc gctttcgctg
    25681 gccaacggga atgccgcagc tggcggcgct attgctggtg ctgctggtcg atagcctggt
    25741 agccccgcat ttctggcagg tggtgcttca ggatgggcgt ttgttcggta gccctatcga
    25801 cattcttaac cgtgcagctc ccgttgcgtt actggcgatt ggtatgacgc tggtgatcgc
    25861 caccggtggg attgatctct ccgtaggggc ggtgatggct atcgccggag ccacaacggc
    25921 tgcgatgacg gtcgcgggat tcagcctgcc gattgttttg ttaagcgccc tgggcaccgg
    25981 catcctggcg ggattgtgga acggcatact ggtagcgatc ctcaaaattc agccgtttgt
    26041 cgccaccctg atcctgatgg tcgccgggcg cggcgtggcg caactgatca ccgccgggca
    26101 gatcgtcacg tttaactcgc cggatctctc atggtttggc agcggatcgc tgttgttcct
    26161 gccaacgccg gtcattatcg cggtgctgac gcttctcctg ttctggctgt tgacccgcaa
    26221 aacggcgctg gggatgttta tcgaagccgt tggtatcaac attcgggcgg caaaaaatgc
    26281 cggggtaaac acgcggatca tcgtcatgct cacctacgtg ttgagcgggc tgtgtgcggc
    26341 gattgcgggc attatcgtgg cggcggatat tcgcggtgcc gatgccaata acgccgggtt
    26401 atggctggag ctggacgcca ttcttgcggt ggtgattggc ggcggatcgc tgatgggcgg
    26461 gcgctttaac ctgctgctct cagtcgtggg ggcgctgatt attcagggga tgaacaccgg
    26521 aattttactt tctggctttc cgccggagat gaaccaggtc gtaaaagcgg tggtggtgct
    26581 ttgtgtgctg attgttcagt cgcaacgctt tatcagtctg attaaaggag tacgtagcca
    26641 tgataaaacg taacttgccg ctgatgatca ccatcggcgt ctttgtgctg ggctatctct
    26701 actgcctgac ccagtttccc ggttttgctt ccacaagagt gatctgcaat atcctgaccg
    26761 ataacgcctt tcttgggata atcgccgttg gcatgacctt tgtgatcctc tctggcggta
    26821 tcgatctctc cgtcggttcg gtgatcgcct ttactggcgt gtttctggca aaagtgattg
    26881 gcgatttcgg cctctcgccg ctgctggcgt ttccgctagt gctggtgatg ggctgtgcct
    26941 ttggcgcatt tatggggctt ttgatcgacg ccctgaagat cccggcattt atcattaccc
    27001 tggcaggaat gttctttttg cgcggcgtca gctatctcgt ttcagaagag tcgatcccga
    27061 taaaccatcc catttatgac acgctctcaa gccttgcgtg gaaaatccct ggcggcggtc
    27121 gcttaagtgc gatgggactg ctgatgctgg cggtggtggt tatcggcata ttcctcgcgc
    27181 atcgtacccg ttttggtaat caggtatacg ccattggcgg caacgcaacg tcggcaaacc
    27241 tgatggggat ttctacccgc agcaccacta ttcgcattta tatgctctcc accggactgg
    27301 caacgctggc ggggattgtc ttctcgattt atacccaggc cggatatgcg ctggcgggcg
    27361 taggtgtgga actggatgct atcgcctcag tggtaattgg cggtacgctt ttgagcggtg
    27421 gcgttggaac ggtattaggg acgctttttg gcgtggcgat tcaggggcta attcagactt
    27481 atatcaactt tgacggtacg ctgagttcct ggtggacgaa aatcgccatc ggcattttgc
    27541 tgttcatttt tattgcctta cagcgtggat taacggtgct gtgggagaat cgtcagagtt
    27601 cgccagtgac aagagtcaat attgcgcagc gataaaaacg cctctccgtg tggagaggcg
    27661 caggagatta cgcgtccggg aattcacgga taaagcgttc gacatcttca accatatggt
    27721 cgttgccgac aaagaacgaa cggcgctggt gcagagtttc cgggatgata tccagaatac
    27781 gctctttgcc atcgctcgct ttaccgcccg cttgttccgc caggaatgcc atcgggttgc
    27841 actcatacag caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg tagagataaa
    27901 taccgccttt cagcaggtta cggtggaaat ccgcgaccag tgaaccgata taacgtgagg
    27961 tatacgggcg gttggtggat ttatcttctt cctggcagaa tttaatgtac ttcttcaccc
    28021 cgttcggaaa cttaatgtag tttccttcgt tgatggagta ggttttgcct ttctccggga
    28081 agcgcatccg ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa gtaaaggcgt
    28141 gaacaccgca tccggtggtg taaaccagca tggtagagga gccgtatacc acgtaacctg
    28201 ccgcaacctg tttgttacca ggctggagga aatcttcttc cgttaccggc gtgccaacag
    28261 gcgtaacgcg gcggtagatg gagaaaatgg taccgacaga gacgttaaca tcgatgttgg
    28321 acgagccatc cagggggtcc atcagcacca cgtattttgc gtgttcacag ccttcaaaga
    28381 cgacaatctc atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt gctttcagtg
    28441 cggctttcag tttttcatta gcgaacaagt cgagtttctg ctgaacctcg ccctgcacgt
    28501 tctcagcacc gctggcaccc aggatatcaa ccagtcctgc tttgttgata tcgcgatgga
    28561 taatcttggc gcccagtttt attgccgaca gcaaagcagt gagctcaccg gtagcatgag
    28621 aaaactcgtg ctgcttttcg acaataaatt cacctaacgt tttcataaaa ctttccctgc
    28681 aatgtttatg gagtaaagcg accgcaacaa tcttaacaaa taatctcaat gttgcgctca
    28741 ggtgaatcgc gccagcaaat tgcggattat cctgaaatgc gtttctcact tgcccgacat
    28801 atgcgtaaaa tgagcggcag attaaaaaag gatagtgacg tatgcgcatt catattttag
    28861 gaatttgtgg cacatttatg ggcggtctgg cgatgctggc gcgccagtta ggccatgaag
    28921 taacgggttc ggacgccaat gtgtatccgc cgatgagcac cttacttgag aagcaaggca
    28981 ttgagctgat tcagggttac gatgccagcc agctcgatcc gcagccggat ctggtgatta
    29041 ttggcaacgc catgacccgt ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc
    29101 cttatatgtc aggtccacag tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg
    29161 ccgttgccgg tacacacggc aaaactacca ccgcgggaat ggcgacctgg attctggaac
    29221 agtgcggtta caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg
    29281 cgcgtctggg cgaaagcgac ttctttgtta tcgaagcgga tgagtatgac tgcgccttct
    29341 tcgacaaacg ctctaaattt gtccattact gcccgcgtac gctgatcctc aacaaccttg
    29401 agttcgatca cgccgatatc tttgacgacc tgaaagcgat ccagaaacag ttccaccatc
    29461 tggtgcgtat cgttccgggg cagggccgta ttatctggcc ggaaaatgac atcaacctga
    29521 aacagaccat ggcgatgggc tgctggagcg agcaggagct ggtgggtgag cagggtcact
    29581 ggcaggcgaa aaagctgacc actgacgctt ctgaatggga agtcttgctg gatggcgaaa
    29641 aagtgggcga agtgaaatgg tcgctggtgg gcgaacataa tatgcacaat ggcctgatgg
    29701 cgattgcggc ggctcgccat gttggtgtag cgcctgcaga tgccgctaac gcgctgggtt
    29761 cgtttattaa cgctcgtcgc cgtctggagt tgcgtggtga agcgaatggc gtgacggtat
    29821 atgacgattt tgcccatcac ccgacggcaa ttctggcaac gcttgcggcg ctgcgtggca
    29881 aagttggtgg tacggcgcgc attattgctg tgctggagcc gcgctcgaat accatgaaaa
    29941 tggggatctg caaagacgat ctggcacctt cattaggtcg tgccgatgaa gtcttcctgc
    30001 tgcaaccggc gcatattccg tggcaggtgg cagaagtggc agaagcctgc gttcagcctg
    30061 cacactggag tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc gctcagcctg
    30121 gcgaccatat tctggtgatg agcaacggcg gttttggtgg gatccatcag aaactgctgg
    30181 atggtctggc gaagaaggcg gaagctgcgc agtaattcgg cctcagcctg atatagtgtg
    30241 cgtaggccgg ataaggcgtt cacgccgcat ccggcatttg agcatagtgc ctgatgcgac
    30301 gcttgatgcg tcttatcagg cctacaggtg caccgcatcc ggcaaaccat actcacatca
    30361 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
    30421 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agagtacgca
    30481 gctgctgacg atcggcatcg tgccacagat ttaatacctc agcgatggcg tcatcacctt
    30541 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
    30601 gacggttttt cagcttatcc agcgcctgac ggatcggttc tacatcgcgc tggcgcagca
    30661 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
    30721 caatagccgc gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
    30781 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
    30841 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
    30901 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
    30961 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
    31021 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
    31081 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
    31141 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
    31201 cgatggcgcg ctggggatca ctgtttatca ccagaaccgc aaaggtagcg catcatccac
    31261 cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg gcgctggata ttgcccgtta
    31321 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
    31381 agatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
    31441 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatt accaataccg aaggtggcag
    31501 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
    31561 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
    31621 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
    31681 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
    31741 gaaagcgcca gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
    31801 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
    31861 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
    31921 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
    31981 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
    32041 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
    32101 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
    32161 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
    32221 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
    32281 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
    32341 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
    32401 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
    32461 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
    32521 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
    32581 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgaaga
//
LOCUS       DAZFRJ010000032        32509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000032 DAZFRJ010000000
VERSION     DAZFRJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.032"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..2321
                     /gene="pdeF"
                     /locus_tag="ACHKUN_001382"
     CDS             78..2321
                     /gene="pdeF"
                     /locus_tag="ACHKUN_001382"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO6659675.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2360..3901)
                     /gene="ppx"
                     /locus_tag="ACHKUN_001383"
     CDS             complement(2360..3901)
                     /gene="ppx"
                     /locus_tag="ACHKUN_001383"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO6659676.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3906..5972)
                     /gene="ppk1"
                     /locus_tag="ACHKUN_001384"
     CDS             complement(3906..5972)
                     /gene="ppk1"
                     /locus_tag="ACHKUN_001384"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO6659677.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6143..6781)
                     /gene="purN"
                     /locus_tag="ACHKUN_001385"
     CDS             complement(6143..6781)
                     /gene="purN"
                     /locus_tag="ACHKUN_001385"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO6659678.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6781..7818)
                     /gene="purM"
                     /locus_tag="ACHKUN_001386"
     CDS             complement(6781..7818)
                     /gene="purM"
                     /locus_tag="ACHKUN_001386"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO6659679.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8143..8769
                     /gene="upp"
                     /locus_tag="ACHKUN_001387"
     CDS             8143..8769
                     /gene="upp"
                     /locus_tag="ACHKUN_001387"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO6659680.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8855..10144
                     /gene="uraA"
                     /locus_tag="ACHKUN_001388"
     CDS             8855..10144
                     /gene="uraA"
                     /locus_tag="ACHKUN_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO6659681.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHHSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10239..10940
                     /gene="hda"
                     /locus_tag="ACHKUN_001389"
     CDS             10239..10940
                     /gene="hda"
                     /locus_tag="ACHKUN_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO6659682.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11078..11437)
                     /gene="arsC"
                     /locus_tag="ACHKUN_001390"
     CDS             complement(11078..11437)
                     /gene="arsC"
                     /locus_tag="ACHKUN_001390"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO6659683.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11458..12921)
                     /gene="bepA"
                     /locus_tag="ACHKUN_001391"
     CDS             complement(11458..12921)
                     /gene="bepA"
                     /locus_tag="ACHKUN_001391"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO6659684.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13134..14195
                     /gene="yfgO"
                     /locus_tag="ACHKUN_001392"
     CDS             13134..14195
                     /gene="yfgO"
                     /locus_tag="ACHKUN_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6659685.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14233..15081)
                     /gene="focB"
                     /locus_tag="ACHKUN_001393"
     CDS             complement(14233..15081)
                     /gene="focB"
                     /locus_tag="ACHKUN_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO6659686.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15103..17115)
                     /gene="hyfR"
                     /locus_tag="ACHKUN_001394"
     CDS             complement(15103..17115)
                     /gene="hyfR"
                     /locus_tag="ACHKUN_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO6659687.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHALLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17145..17558)
                     /gene="hyfJ"
                     /locus_tag="ACHKUN_001395"
     CDS             complement(17145..17558)
                     /gene="hyfJ"
                     /locus_tag="ACHKUN_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO6659688.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17551..18308)
                     /gene="hyfI"
                     /locus_tag="ACHKUN_001396"
                     /pseudo
     CDS             complement(17551..18308)
                     /gene="hyfI"
                     /locus_tag="ACHKUN_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18305..18850)
                     /gene="hyfH"
                     /locus_tag="ACHKUN_001397"
     CDS             complement(18305..18850)
                     /gene="hyfH"
                     /locus_tag="ACHKUN_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO6659689.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18860..20527)
                     /gene="hyfG"
                     /locus_tag="ACHKUN_001398"
     CDS             complement(18860..20527)
                     /gene="hyfG"
                     /locus_tag="ACHKUN_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO6659690.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20517..22097)
                     /gene="hyfF"
                     /locus_tag="ACHKUN_001399"
     CDS             complement(20517..22097)
                     /gene="hyfF"
                     /locus_tag="ACHKUN_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO6659691.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22102..22752)
                     /gene="hyfE"
                     /locus_tag="ACHKUN_001400"
     CDS             complement(22102..22752)
                     /gene="hyfE"
                     /locus_tag="ACHKUN_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO6659692.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22764..24203)
                     /gene="hyfD"
                     /locus_tag="ACHKUN_001401"
     CDS             complement(22764..24203)
                     /gene="hyfD"
                     /locus_tag="ACHKUN_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO6659693.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24220..25167)
                     /gene="hyfC"
                     /locus_tag="ACHKUN_001402"
     CDS             complement(24220..25167)
                     /gene="hyfC"
                     /locus_tag="ACHKUN_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO6659694.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25178..27196)
                     /gene="hyfB"
                     /locus_tag="ACHKUN_001403"
     CDS             complement(25178..27196)
                     /gene="hyfB"
                     /locus_tag="ACHKUN_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO6659695.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27196..27813)
                     /gene="hyfA"
                     /locus_tag="ACHKUN_001404"
     CDS             complement(27196..27813)
                     /gene="hyfA"
                     /locus_tag="ACHKUN_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO6659696.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28066..28536)
                     /gene="bcp"
                     /locus_tag="ACHKUN_001405"
     CDS             complement(28066..28536)
                     /gene="bcp"
                     /locus_tag="ACHKUN_001405"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO6659697.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28536..29108)
                     /gene="gcvR"
                     /locus_tag="ACHKUN_001406"
     CDS             complement(28536..29108)
                     /gene="gcvR"
                     /locus_tag="ACHKUN_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO6659698.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29254..30132
                     /gene="dapA"
                     /locus_tag="ACHKUN_001407"
     CDS             29254..30132
                     /gene="dapA"
                     /locus_tag="ACHKUN_001407"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO6659699.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30149..31183
                     /gene="bamC"
                     /locus_tag="ACHKUN_001408"
     CDS             30149..31183
                     /gene="bamC"
                     /locus_tag="ACHKUN_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO6659700.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31396..32109
                     /gene="purC"
                     /locus_tag="ACHKUN_001409"
     CDS             31396..32109
                     /gene="purC"
                     /locus_tag="ACHKUN_001409"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO6659701.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32263..32490
                     /gene="vapB"
                     /locus_tag="ACHKUN_001410"
     CDS             32263..32490
                     /gene="vapB"
                     /locus_tag="ACHKUN_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO6659702.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
BASE COUNT         7981 a         8411 c         8499 g         7618 t
ORIGIN      
        1 aatcatcatt cgaagtcagg tgggatgcct gtctgaatac acctccttca ggatgtgggg
       61 gattctgctg agcatctatg aaactgaatg caacttatat aaaaatacgt gataaatggt
      121 gggggcttcc gctgttcctg ccttctttaa tcttgcccat tttcgcccac attaatactt
      181 tcgcgcatat ttcttccggt gaggtttttc tcttttatct gcctttggca ctgatgatca
      241 gcatgatgat gtttttcagc tgggcggcat tgccagggat caccttaggg atttttgtcc
      301 gcaaatatgc agagctgggt ttttacgaaa cgctctcatt aacggctaat tttattatca
      361 ttatcattct ctgttggggc ggttacaggg tctttactcc ccggcgtaac aacgtttcac
      421 atggtgatac ccgtttaatt tcccagcgta tattctggca gattgtgttt cctgcaacgc
      481 tgtttctgat acttttccag tttgctgcat ttgtaggatt actggcgagc agagaaaatc
      541 tggtcggtgt catgcctttt aacctcggga ctttaatcaa ttatcaggcc ttactggtgg
      601 gtaatctgat cggtgtcccg ctgtgctact tcatcattcg ggtagtgcga aatccatttt
      661 atttacgtag ctattattcg caattaaaac agcaggttga tgccaaagtc accaaaaaag
      721 agttcgcgct ctggctactg gcattaggtg ctttattgtt gctgttatgc atgccgttaa
      781 atgaaaaaag cacaattttt agcaccaatt ataccttgtc attattgctg cccctgatga
      841 tgtggggagc gatgcgctat ggttataagc tgatttcgct gctctgggcg gtcgtgttga
      901 tgatcagcat ccacagctat caaaattaca ttcccattta tcctggctat accacgcagc
      961 tgaccataac ctcctccagt tatctggtat tctcttttat tgtcaattat atggctgtac
     1021 tggcaactcg tcagcgagcg gtagtcagac gcattcagcg gcttgcgtat gtggacccgg
     1081 tggttcatct gccaaatgtt cgcgccctga atcgcgcgtt gcgtgatgcc ccctggtctg
     1141 cgctttgtta tttacgcatc cctggcatgg aaatgctggt taagaactat ggcatcatgc
     1201 tgcggattca atacaagcaa aaactttctc actggctgtc acccttgctg gaaccgggtg
     1261 aagatgttta tcagctttcg ggtaacgatc tcgcgctgcg gctgaataca gaatcgcacc
     1321 aggagcgcat taccgcactg gatagccatc tcaagcaatt tcgtttcttt tgggatggca
     1381 tgccgatgca accgcagatt ggcgtcagtt actgctatgt gcgctcgcca gtgaatcata
     1441 tctacctgct gctgggagag ctaaatacgg tcgccgaact ttccatcgtg accaacgccc
     1501 cggaaaatat gcagcgtcgc ggggcaatgt atttgcaacg cgaattgaaa gataaagtcg
     1561 cgatgatgaa tcgactacag caggcgctgg aacacaacca ttttttcctg atggcccagc
     1621 cgattaccgg tatgcgtggt gatgtttacc atgaaattct tctgcgcatg aaaggtgaga
     1681 atgatgaact gatcggcccc gatagcttct tgccggtcgc gcacgaattt ggtttatcgt
     1741 cgagtatcga catgtgggtc attgagcata cgctgcaatt tatggctgaa aacagagcga
     1801 agatgcccgc tcaccgtttt gctattaatc tgtctccaac ctcggtatgt caggctcgtt
     1861 ttcctgttga agtcagtcag ttgctggata aatatcagat tgaagcgtgg caacttattt
     1921 ttgaagtcac cgaaagtaat gctctgacca atgttaagca ggcgcaaatc accttgcagc
     1981 atcttcagga attaggctgc cagattgcga ttgatgattt cggcaccggc tacgccagct
     2041 atgcgcggct taaaaatgtg aatgccgatc tgcttaaaat tgacggcagt tttatccgca
     2101 atattgtgtc aaatagtctg gattatcaga tagtggcatc gatttgccac ctggcgcgaa
     2161 tgaagaaaat gcgggtagtg gcagagtacg ttgaaaacga agagatccgc gaggcggtgc
     2221 tctctttggg gatcgattat atgcagggtt atcttattgg taagccgcaa ccgttaattg
     2281 atacgctgaa tgaaatcgaa cccattcgcg aaagtgcctg aataatgcgg gccgacattt
     2341 ctcgtcggcc cgcaaagcat taagcggcga tttcaggtgt actttcttct tcaattttca
     2401 accgccagcc agccacgcct tcccagtatt cttgctcctt ttccagatca agcagtacca
     2461 gcgcattctg actaaaccag tcatgcggga aacgcagtgt ccagtgactg tcatcagtaa
     2521 tcagtgtcaa tgttggcggt gtggtggttg cctgacgttg attgtttagt aatacgccaa
     2581 ggcgcaatag ctgtatcagt ggcaggaact gtttcttctt aaacaaggta aagcgcggca
     2641 gatcgtcgag cttaatcgct ttacggtgat agcgcaccag tgtcgccatc atcagctgct
     2701 gttcctgatt aaaacccggc aagtcactgt tttgcagaat ataagcggag tggcgatgca
     2761 aaccgctgtg gttgatattc aacccgacct catgcagcat ggcggcccat cgcagtagcg
     2821 cctccagttg cggatgcgcc agcttcggtt gctgttcccg ccactgttcg tacatttgca
     2881 tagtggtatc cagcactcgt cgggcctgtt cgctgtcgat gtgatactgg ttggcgaggc
     2941 tgctggcggt gcgactacgc acatcctgat gacggaaacg tccttccatt tcatacagta
     3001 cgccttcgcg taacgccccg tcagaaaggc gcagttcacg gatggctaaa gcatcaaaca
     3061 caccacataa aatcgccagt ccaggaacga agactgtttt ccgctcttcg gaaagacccg
     3121 gtaaactcag cgatgcgaaa ttacggtgac gtaaaacttc ttttaccagt ttttccagac
     3181 gttccggggt aattatcccg tctttctcgc ccatttccat taatacttca tgggcggctt
     3241 ttatggtacc ggaagcgccc attgccacgt tccagccctg aatacggaat tgccaggtta
     3301 aagtttccag tttttgtgct gccgccatgc gagcgcgctg aaaattctct ttattgatga
     3361 ccccgccagg gaaataaagc tgggcaaagc tgacacaacc catccggcgg ctttcaacga
     3421 gaataggttc gaaattttca ccaatcacca gttccgtaga tccgccgcca atatcaataa
     3481 ccagtttgcg acctttttcc ggttgggtat gttccacgcc cataaaaatc agacgggctt
     3541 cttcattacc ggaaataatt tcaatcgggt agggaatgac cttttccgcg cgtttcagaa
     3601 agtcagtggc gttcagcgcc tgacgcagtg tatgggtgcc aactatacag acgctggcag
     3661 gagaaaaccc ttgtagccgt tcggcaaaca gcgacagaca gttcaaaccg cgcgtcattg
     3721 cctcttcact caacatatta tctggcccca ggccgtccgc cagatgcacc cgctgtttca
     3781 ggcggccaat aatctgcatg gcaccatcta ccacacgggc tatgaccatg tgaaaactgt
     3841 ttgaaccaag atcgaccgca gcaaactcct gcggacgagg ggatttatcg tgtattggca
     3901 tagggttatt caggttgttc gagtgatttg atgtagtcat aaatcgccaa ctgcgcccgt
     3961 actttgcggc gattgccgcg gggaacgtag cgattactga gttctttatc gatataacgt
     4021 gctttgaccg tatcgctgaa caatatgtcg atgatgtcca gcacccgctg cttcaggcgc
     4081 ggatcgagca gcggtgtcgc cacttcaata cgataatcaa tattgcgcgt catccagtcg
     4141 gcggaagaaa ggtagacctt tttatcgccg ccattttcaa aaatataaac ccggtcatgt
     4201 tcaaggtaac ggtcaacaat actgatggca cgaatgttgt cgctaatgcc ttccagattg
     4261 gggatcagcg aacacattcc gcgaaccagc agattaaccg gtacgccgga gctggaggcc
     4321 gcatacagac gatcaaccag gcctttatcg acaaggttat ttagcttcag ggtgatacca
     4381 ctgggcagcc cttgctgcac gttggcgatt tcgcggtcta ccatttcata taacagacgg
     4441 cgagagtttt gcggtgacac cattaaataa tcaaatgtca ctgggcggta tgggttttca
     4501 ataaagttaa atacccgccg tacttcgttg gtgatacgcg catctgcggt cagcaacgaa
     4561 tagtcagtat aaagacgcgc ggttttttcg ttaaagttcc cggtcccgat gtgtgcgtaa
     4621 cggaccactt caccgttttc tttacgtgaa atcaggaaca gtttggcgtg aattttcagc
     4681 cccggcgcag agaagataac gtgcacgcct gcttcggtca ggcgcttcgc ccagtgaatg
     4741 ttggcttctt cgtcgaaacg cgcctgtaac tcaaccacca cagtgacttt cttaccgtta
     4801 tgtgcggcgt ggatcatcga gtcgatgatg cgtgaatctt tcgccacgcg gtaaatgtta
     4861 attttaatcg ccagtacgct cgggtcgaac gaagcctgac gcagcagttc cagcacatgc
     4921 tcaaaggtgt gataaggata atagagcaac acatcgcgtt cgcgaatggc atcaaaacca
     4981 ttgcggaact gggctttatc aaaccaaatg tggcgtaaac gcggcagtgg tttgttcacc
     5041 agattggctt tgccgacatt ggggaaatta ataaagtctt taaaattatg ataacgaccg
     5101 ccggggacga tggagtcgta gcgggaaata gtcagttttt cgcgtaacac ttcaaccagc
     5161 gcattgggca tatcgcgctg ataaacaaaa cgcaccggct cagcagttaa acgctgcttg
     5221 agactggaag acatcaactc catcaggctg gcttccatct catgcactaa atcgtattcg
     5281 gcatcgcgtg tcatcttcat tgaataggca ttcagcgcgt cataatcaaa gaagcctttg
     5341 aaaatatcat caaggcagta acgcagaatg ttatccagaa gaatcatcgg cttgcgtcga
     5401 cgcggcgctt ctggcggtaa attcacaaag cgcggcactt tatctgatgg gatctccagc
     5461 agcgcgtaac ggatggtatc accacggata atttccaccg ccagataggt gtaatcatcc
     5521 ttcaggaact gcactaagtc agtgtcagga ttgattaaaa tcggcgtaat gtgctgacgc
     5581 agatactgct taaaataatg acgaagccag ttttgttgat tgacggagag ctggcgttca
     5641 ttaatcagga agatctggtt gcgcgccatc tccagcaata gctcgttgta gaggccgtcg
     5701 aattcctgat cggctttcag cacccgggac tgaattttgc ccagtaaatg gcgggaatga
     5761 gagttggagc cttgttcttc gctaataatg atgcgtcgct tcagttcagc gaagcggact
     5821 ttatagaact catcaaggtt attggaatag atccccagga aacgcatcct ttcaatcagc
     5881 gggttagatt tgtccgccgc ttcctgaagc acgcgttcat tgaacgataa ccaactgagc
     5941 tctttttcga tgtatagctt ttcctgaccc attaccactt ttactccgtt ttattcacgg
     6001 gacactgcct ggatattatg gcgagcaata ttggcagatg tccaactatg ccagaaaagg
     6061 gggaaaaaaa ctgctggagc ttgcgattta caggtgaatt gaaaaacgcc agcggcagag
     6121 ctggcgcttt aactacgggg cattactcgt cggcagcgta gccctgcggc ggcagacgtt
     6181 gaccatccag ccacgcggcg ttttcgtgca ttttcagacg accatcggca aaccagctaa
     6241 tcaccagtgg ataaatagcg tgttcctggg tttgcacgcg ggcggtgatg tcatcttccg
     6301 tatcaccagc aaataccggg actttcgcct gtaaaataac cgggccaccg tccagttcat
     6361 cggtgacgaa atgcaccgat gtaccgtgct cttcatcacc attttccagc gcttgacgat
     6421 gggtgtgtaa tccgggatat ttcggcagca gagaagggtg aatgttcagc aaacgcccgg
     6481 catagtggga gacaaacgcc gggctgagaa tgcgcataaa accagccagc acgaccacat
     6541 cgggtgcgta catgtcgatt tcatgaatca actcccggtc ataggcttca cgactgtcaa
     6601 acgcgctggc gatgagcgta tgcgttgcaa tacccgcctg gcgggcgcgt tcaaggccga
     6661 acgcgtcggc cttattgctg aaaactgccc gtacggtgcc tttaattttg ttggttttac
     6721 aggcgtcaat aattgcctgt aaattacttc cgttaccgga aataagcacc acaatattca
     6781 ttattcgata accacgcgtt gttcggaatc agaggctttg atgataccga ttttccacgc
     6841 gttttcaccg ttggcattga gcagggcgag ggctttgtcc acttccggag caggcagggc
     6901 aataatcatc ccgacgccgc agttgaaggt gcgatacatt tcatggcgct caacgttacc
     6961 tgccgtttgc agccagttga acacttccgg ccactgccag gaagattcat caatcactgc
     7021 ctgagtatta tctggcaata cgcgtggaat gttttcccag aagccgccgc cggtcaggtg
     7081 cgcaatggca tgcacatcga ccttttcaat caactccagc actgacttca cgtaaatgcg
     7141 ggtcggtgcc agcagataat cggctaatgg cttaccatca agttgggtgg tttgcggatc
     7201 acaaccgctg acttcaagaa ttttgcgcac cagcgaatag ccgttcgagt gcggaccgct
     7261 ggaaccgagt gcaatcagca catcgccgtc gctgacttta gagccgtcga tgatttctga
     7321 tttttctacc acgccaacgc agaaacccgc gacatcgtaa tcctcaccgt gatacatccc
     7381 cggcatttct gccgtttcgc cacccaccag tgaacagccc gattgcagac atccttccgc
     7441 gataccgctg atcaccgctg aagcggtatc aacatccagt tttccggttg cgtaatagtc
     7501 gaggaaaaac agcggctctg caccttgcac caccaggtca ttaacgcaca tggcgaccag
     7561 atcaatacca atggtgtcgt gacgttttaa gtccattgcc agacgcagct tggtacctac
     7621 gccgtcagtg ccggaaacca gcacgggttc acgatatttt tgcggcaatg cacacagcgc
     7681 accgaagccg cccagaccgc ccatcacttc cggacgacgc gttttcttca ctacgccttt
     7741 gattcttcca accagagcat tacccgcgtc aatatcaaca ccggcatctt tgtagctaag
     7801 agaggtttta tcggtcactg cttgggtccc cacgcgttac ttgcggtaga aaaataaaat
     7861 tcggcgcaat tctaacaggg aaagcaaacg tttgcgagac tgctttacac aacctttttg
     7921 cacgtctttt gcccaggcgc gcggcgaaag aagacttgta ccagggtaaa ggttagtttt
     7981 cggatggaat aatcttcttt cataaccatt tgaatataaa ataactttat ctcaagccgt
     8041 tatcattttg actaaagtca acgaaaagaa tattgccgcc ttgaagaaag gaggtataat
     8101 ccgtcgattt tttttgtggc tgcccctcaa aggagaaaga gtatgaagat cgtggaagtc
     8161 aaacacccac tcgtcaaaca caagctggga ctgatgcgtg agcaagatat cagcaccaag
     8221 cgctttcgcg aactcgcttc cgaagtgggt agcctgctga cttacgaagc gaccgccgac
     8281 ctcgaaacgg aaaaagtaac tatcgaaggc tggaacggcc cggtagaaat cgaccagatc
     8341 aaaggtaaga aaattaccgt tgtgccaatt ctgcgtgccg gtctggggat gatggacggt
     8401 gtgctggaaa atgttccgag cgcgcgcatc agcgttgtcg gtatgtaccg taatgaagaa
     8461 acgctggagc cggtaccgta cttccagaaa ctggtttcta acatcgatga gcgtatggcg
     8521 ctgatcgttg acccaatgct ggcaaccggt ggttccgtta tcgcgaccat cgacctgctg
     8581 aaaaaagcgg gctgcagcag catcaaagtt ctggtgctgg tagctgcgcc agaaggtatc
     8641 gctgcgctgg aaaaagcgca cccggacgtc gaactgtata ccgcatcgat tgatcaggga
     8701 ctgaacgagc acggatacat tattccgggc ctcggcgatg ccggtgacaa aatctttggt
     8761 acgaaataaa gaataaaaat aattaaagcc gactttaaga gtcggctttt ttttgagtaa
     8821 agcgcctata acacataata cagaggataa tactatgacg cgccgtgcta tcggggtgag
     8881 tgaaagaccg ccacttttac agacaatccc gcttagtttg caacatttgt tcgccatgtt
     8941 tggtgcaacc gtcctggtgc ccgtcttatt tcatattaac ccggcgactg tactgttatt
     9001 taacggtatt ggaacgctgc tgtatctctt catctgtaaa gggaaaattc cggcttatct
     9061 tggttccagc tttgccttta tttcaccggt attgttactg ttgccgttag ggtatgaagt
     9121 cgcgctgggc ggctttatta tgtgcggcgt gctgttctgc ctggtttctt ttatcgtgaa
     9181 gaaagcgggg accggctggc tggacgtgct gtttccacct gcggcaatgg gcgcaatcgt
     9241 tgccgtcatc ggtctggagc tggcgggcgt agctgccggt atggcgggtt tactcccggc
     9301 tgaagggcaa acgccagact ccaaaaccat catcatctct attaccaccc tggcggtcac
     9361 ggttttaggt tccgtgctgt ttcgtggttt cctggcaatt atcccgattt taattggcgt
     9421 gctggtgggg tacgcgctct ctttcgcaat gggaattgtc gataccacgc cgattattaa
     9481 tgctcactgg tttgcgctgc caaccctcta tacgccgcgc ttcgagtggt ttgccattct
     9541 gactattctg ccagcggcgt tagtggttat tgccgaacac gtagggcacc tggtagtaac
     9601 ggctaatatc gtcaaaaaag atctgctgcg cgatccaggt ctgcaccatt cgatgtttgc
     9661 taatggcttg tcgaccgtga tttccggctt ctttggctct acgccaaata ccacttacgg
     9721 agaaaacatt ggcgtgatgg cgatcacccg tgtttacagt acctgggtta tcggcggggc
     9781 ggcgattttc gctatcctgc tttcctgcgt cggtaaactg gctgccgcta tccagatgat
     9841 cccattgccg gtgatgggcg gcgtttcgct gctgctttat ggtgtcatcg gtgcttccgg
     9901 tattcgtgtt ttgatcgaat cgaaagtgga ttacaacaaa gcacagaacc tgatcctgac
     9961 ttccgtgatt ttgatcatcg gcgtcagtgg cgcgaaggta aacatcggcg cggcggagtt
    10021 aaaaggtatg gcgctggcga ccatcgtcgg tatcggctta agtttgatct tcaaactgat
    10081 cagtgtgctg cgccctgaag aagtggtgct ggacgcagaa gacgcggata taacagacaa
    10141 ataatgtacc gaccgggcag tgttgctgcc cggttcaaac atcatgggat tctgtggtaa
    10201 acttctcgcg attttgtgaa atcctggttg aggtatctct gaacacaccg gcacagctct
    10261 ctttgccact ttatcttcct gacgacgaaa cctttgcaag tttctggccg ggggataact
    10321 cctctttact ggctgcgctg caaaacgtgc tgcgtcagga acatagcggt tacatctatc
    10381 tctgggcacg cgaaggcgcg gggcgcagcc atctgctgca cgcggcttgc gcggaattgt
    10441 cgcagcgtgg cgatgcggtg ggctatgtcc cgctggataa acgcacctgg tttgttccgg
    10501 aagtgctcga cggtatggag catttgtcgc tggtctgtat cgacaacatt gagtgtattg
    10561 caggcgatga gttgtgggag atggcgattt tcgatctcta caatcgaatt ctggaatcgg
    10621 gcaaaacacg attgttgatc accggcgatc gtccaccgcg gcagttgaat ctgggattac
    10681 cggatctcgc gtcgcgactc gactgggggc agatctacaa attgcagcca ctttctgatg
    10741 aagataagtt gcaggcgcta cagttacgcg cgcgtttgcg tggttttgaa ctgccggaag
    10801 atgtggggcg tttcttgctg aagcggctcg acagagaaat gcgcacgcta tttatgacgt
    10861 tggatcagtt ggatcgtgcg tcgattaccg cgcaacgtaa gctgaccatt ccgtttgtga
    10921 aagaaattct gaagttgtag ataaggtgtt tattgtcgga tgcggcgcga acgccttatc
    10981 cgaactacgc gaagttaatc catagtattc gtaggccgga taagacgtgg caagcgtcgc
    11041 atccggcaat gtgcttcaac tagcgatgga gcacatctta acccacgatt tccagtacct
    11101 gctccggtgg acgaccaatc cgcgctttgc cattcgcgac cacaatcggg cgctccatta
    11161 gcttcggatt atccaccatt gcctgaatca gcgcctcttc gctaagcgaa ctgtcagcca
    11221 ggttcagttc tttataaaga tcctctttct ggcgcatcaa ttcacgggcg ctattcatcc
    11281 ccagcatttt cagcaaatcg cgcagcgttg ccgcatcggc gggtgtctca aggtagagta
    11341 ccacttctgg ctccacgccg ttttctttca gcaggttcag cgtttcccgg ctctttgaac
    11401 agcgtgggtt atggtagatt tttacctgtt tggtcattgt tcttccttta atgcgaatta
    11461 catcttggta taaggcttaa agcgttcctg cagctggcgc aactggtcga tgcgcgcatc
    11521 gtaacgcgct tgttgcaggc tgcctaattt cacctgcgaa ctggcgctac tcaacagcga
    11581 aatggcctga tcgagtcgtc cggcgagcgc ataaccttct gctcgcgcag ccagctcctg
    11641 atcgcggtta tttagcgcgg cttccgcctg tgccagtaaa tcccagccgt tgctgtcatc
    11701 tttattatta aaggtgtagc gattcagaat attggccgct tcttgtggtt gaccgccttg
    11761 tagataagcg ttcgccaggt tgagctgcaa caccggattg gtgcgcaaat cacgggcatt
    11821 tttcaggcga ttgattgcct cattggcttt gttttgccca agatcgatat cggtagccag
    11881 atcgagatac catgcgttgc caggttctgc cgccagtaac ggttgcagcg ttttacgcgc
    11941 ctcgtcgtat ttattggctt ccatcgcctg taaagcacga ccatattgcg ccgctcgttg
    12001 ctgacgaacg tttcctttcg cccattcatc cagcaaatca ctggtgagct ggttacgtcc
    12061 ggaattatac atccccagtg tgcgcgcttt cgccagatag aaatcttccg acgactgcac
    12121 caccatcggt cgcatctgat tagcacggtt gcgggcatct gccagacgac tttccggcaa
    12181 cgggtgagtc aataaaattt ccggcgggcg cgaggagtaa cgcgcctgat cgagtaattt
    12241 ttcgaggaag gttggcatcg cctgcggatc gaatcccgag cgttgcagca cctgaatacc
    12301 aatgcggtcc gcttcctgtt cattttgctg ggtgaaactg atcattccct gacgtgttcc
    12361 cgccagtgta ccggtcagcg ccgccatccc cgcctgcgga ctggccatcg ccagtaaaat
    12421 agaacctaac gcgccgaccc aggtcagcgg cgcgctgcgt tgttgatctt ccatcgctcg
    12481 cgccaggtga cgttgggtga cgtgggagat ttcgtgcgcc ataactgaag ccagttgact
    12541 ttcgttatcg gaataacgga acagggcaga gtgcagcacc acgttgccgc caaagaaagc
    12601 aaaggcgtta atttcgtcgt tgttgatcag aaaaaaatga aacggtgtct taaccgaatt
    12661 ggcatgcgaa accagacgca tccccagcga attaatatat tgcgttaaca gcgggtcatt
    12721 aattaacggc gcgctgccgc gtagctggcg gacataatag tcgcccatct gcatttcctg
    12781 accaatggaa agcgtgcttc ctgcggaggt tcccatatcc ggcaaggtgt ctgcgctgtc
    12841 ggcaaacgcc ggggctacct gaccaatggt catagcagca atgagggttg caaccaggtt
    12901 ttttttcaac tgcctgaaca taacctctat cctgtatttc tgaagatagt catttgaccg
    12961 atactgttaa atatcgttcc caccgcgccg cctttgagtg taacggcgaa gattagccca
    13021 ctcaaatgac ttttcagttt cctgaatccg tcataaaaag cgaagtcttt tttgtgacat
    13081 ttcgatacaa ttgcggatcg caatcccgct cttgaggttc agggaaggtt tttatgctcg
    13141 aaatgttgat gcaatggtat cgccgccgtt ttagcgaccc ggaagcgatt gccttgctgg
    13201 ttattttagt tgccggattt ggcattatct ttttctttag tggcctgctt gctccgttgc
    13261 tggtggctat tgtgctggcc tatttgctgg aatggccaac cgtgcgcctg caatctattg
    13321 gctgctcccg ccgctgggcg acgtcgattg tattggtggt ttttgtcggt atattgctac
    13381 tgatggcgtt cgtggtactg cctatcgcct ggcaacaggg catctactta atccgcgata
    13441 tgccggggat gctcaataag ctttctgact ttgccgccac gttgccgcgc cgctatccgg
    13501 cgttaatgga cgcgggcatt attgatgcaa tggccgaaaa tatgcgcagt cggatgctga
    13561 ccatgggcga ttcggtggtg aaaatttccc tcgcctcgct ggtcggtttg ctgaccattg
    13621 ccgtctatct ggtgctggtg ccattgatgg tcttcttcct gctgaaagac aaagagcaga
    13681 tgctgaacgc cgttcgtcgg gtgctgccgc gtaaccgtgg gctggcagga caggtgtgga
    13741 aggagatgaa tcaacaaatc accaactata tccgcggcaa agtgctggag atgatcgtgg
    13801 tagggatcgc cacctggctg gggttcttgc tcttcgggct gaactattcg ctgctgctgg
    13861 cggtgctggt cggcttctcg gttcttattc cgtacattgg cgcatttgtg gtgaccattc
    13921 cggtggttgg cgtggcgcta ttccagtttg gcgcaggcac ggaattctgg agctgcttcg
    13981 cggtgtatct gattattcag gcgctggacg gcaacctgct agtaccggtg ttgttctccg
    14041 aagcggttaa cctgcatccg ctggtgatta ttttatcggt ggtgatcttc ggtggtttgt
    14101 ggggattctg gggcgtattc ttcgccattc cattggcgac gctgatcaaa gccgtgatcc
    14161 acgcctggcc cgatgggcaa atcgcgcaag aataatcttc agcccgacga actgtttcgc
    14221 cgggctgatt tttcaggatt cctgacgtaa ataaatagcc cgataacaca tactcaccag
    14281 cactgcaccg ccgataatat tacccagcat caccgggagc agattggcgg taataaaatg
    14341 gctgaccgtc agtgccggaa aattgtctgc gctactgtgc gccagttgcc agaaggaggt
    14401 aggggcgaaa tggcgaatgg caatggcgaa cggaatcaca aacaaattgg cgatacagtg
    14461 ctcaaagcca ctggcgacaa acagggtgat gggcaaaatc atggcgacga ttttgtcgca
    14521 taacgaacgc ccgcaataac tcatccacag cgccaggcaa accatcagat tgcacataat
    14581 gccgaggctg acagattcag taaatgtatg atgcattttg ccctcggcgc agtgtaaaac
    14641 cgcgactccc cacatcgcgt tttcactcat caccagcccg gaaaaccaga ttaacaaact
    14701 gaaacaggca atacctgcca gattaccgca ggccaccaga agtgcgttaa tcagccaagt
    14761 tcgccaacta ataacgcccc tactttttgc cattaccgtc attaccgacg aggtgaacag
    14821 gttggtgccg caaaccgcca gcaaaataaa gccaagtgta aagcaaaggc cgcccaccag
    14881 atgggttaat gcctgtgaag acggggcatc ggcgataact gaaaggtaaa aagtaaatcc
    14941 gatcgccata aacacgccag cggacatcgc cattaaaaag accgacactt tactgcgggc
    15001 aattttatgg gcggcaatcc gttcagcgat tgccgctttt ctggcgctca actgcaagtc
    15061 gaaagagagt ttgtttctca tgcatactcc tgcaaaagca gattacaaca cctcgcgaac
    15121 cgagattccc agacgctgca ttcgtgacag cagcgtggtg cgcttcatcc ctaatcgtgt
    15181 cgccgcgcca cggggcccgg caacaatgcc attggtttct cgcaatacct gaacaatgcg
    15241 ctgacgttct tcttcgtcat tctctggcgt cgtcgggtgc agcaactgcg ccattgaact
    15301 gcgaagcgct gaatcttcat ttagcgtcgg cagtaaacgg ctttgtcgga cattaagatg
    15361 taaattcaga ctgttaccgc gagtcagcag taccgctcgc tcaatcacgt tttccagctc
    15421 gcgcacgttg cccggccagt cccacgacat caactggcgt agcgcctcgg tcgggatggc
    15481 gtcaattgaa cgattcatat ggcgcgccat tttttgcgtg aaatgctttg ctaaaagagg
    15541 gatatcttcc ggacggtcgc gcagcggcgg caattccagt gggaagacat tcaggcgata
    15601 aaagagatcg ctgcgaaact ggcgatcttc aaccatttgc cacaaatcac ggttggtggc
    15661 ggcaatgact ctgacattta ccgggatcgt tctactcccg ccgagacgct caatctcccg
    15721 ttcctgcaat acgcgcagca gtttaggctg aagttctaac ggcagatcgc caatttcatc
    15781 gagaaacaac gtgccgccat cggcaatttc aaaacgacca cgatgggtat taatcgcacc
    15841 agtaaacgcc cctttgtcat gaccgaataa ctcactttcc agaagactgg cggggatggc
    15901 agcgcagttg attttgacca gcggcttgtc gcgtcgcggg ctaagttgat ggatcgctct
    15961 ggcgatcacc tctttgccgg ttccggtttc accgcaaatc aacaccgtac tgtcgctctt
    16021 cgccacaata tctacctgct ggagtaggtc ttccattgcc tggctttgat agataatgtc
    16081 accgatgccc agattcgaaa ggagctgctc gctaagctgg tggttttctt gctgcaaact
    16141 ttcctgcaaa tcggtcatgc tacgccaggc atcggcattg ccaacggcaa tagcgatgcg
    16201 atcggctatg tgttgtagta gctggcagtt ttcctcacta aagagagtgg aagaggtatg
    16261 cgccagcaac aatgcacccg gtgtatggtt gccaaaggta agcggtataa ggagcgcaga
    16321 ttcgcagccg ttagatgcaa gtaagagtaa taacgggtcg cgttgccaga gaaacagatc
    16381 gtctgcacgg tgcgtcaagg tcggttgtcg ggtttgtagc gtttgtgtca gcaatacact
    16441 gtcgccaggc atattgcgtg gcagacacgc acaatgtgag gcagaaagat cgctgcacca
    16501 caggctaaac ttctcgttct ttcgatgatc gcccagtacc atactgactg aagccagacc
    16561 gaaaaaatga tggatctcac gagcgacgtc agcgatcaga tcgtcgagat caagatgtga
    16621 cagcactgca ttagtaatat caaccagtac ccgatgatga tcgcgatctt tctccagcaa
    16681 acgcagcgcc acctgctgtt cttcatcatt gagccatgat tgcagaaccg gggtgattat
    16741 ccgcgccagc cagttaaaca gtgtgcaatc ctggggagta aaacggctgc cgtgggtacg
    16801 aataaagcgt agctccgcca ttccactact gccgagaagg agggcgtgac gcgaataatc
    16861 gaccatctcg ccctgatact ctgttgttgc cgtcacatcg ctgccatcat catggtagcg
    16921 gtaatagaag ttgcttttga gaacatgcag ttcaataagt tgtaccgggc tgaacggcgg
    16981 cggcatcggg atgaaggcgc gtaataaaga cgccttaccg tgtttctgtg ctaaccgctc
    17041 cgcgagcatt ccgtttaccg cttcaattgt tattccttgt ggcggggcaa acatcgcctc
    17101 gtctgacata gccatcaaca gtccttatta atgagaaatc cttctcacgc aatttttctg
    17161 gccatcagat agatggccgg ttcctgcaca atttcatcca acagatgaat gaattgaatg
    17221 ctacgcgcct gccacacaga cgtcgcgtca tccgccagtg gcgcgaatgc ccgggtcaac
    17281 agggcggtgt ggctggcgtc aataacaatc tcaccaaaag tcatcacccc cagcatctta
    17341 cgtcgggctt gctcctcttc gacctgggca agccaatctt cgtattccgt cagtgggcag
    17401 gggaaggcga cattcagaca atcaatcacg ccaacgtgat gaccgatagc cagcgagtaa
    17461 tacattacct gagagctgtg ttccggcatc tcgtcgctac tggcgacaaa cttttttcgc
    17521 agcgtccaga aaacaatttc cccgcactct tcagtcatgt aacccccgga gtacgcgaaa
    17581 gagttgctga acgatactat tgagacgtgg atcgtcggca tcgcgcaacc aggtgttaac
    17641 ccgatttcct gtaggatcgt cgcttaaatg gcgcaggagc cgatcgcaaa tttctcgccc
    17701 ctgacgatag cccgccagcc gccgcgcttc tcgctcaatg gcgatacgct gtgatggcgg
    17761 gatctgcggc cacaacggtt gcatagtcac cccggtggga tcgcgataat ccaccgcgtg
    17821 aatcttctgt tgcagcaaac cgagcgccac ggcgaaaccg tgaatggtgg cggccggtgt
    17881 tggcgggcag ccggggatcc aaacatcaat ggggacaatg gtgtcgctgc cgccccagac
    17941 gctgtagaga tcgtggaaaa tccgccgccg gcaccgcacg cgccgtagga aacacagatt
    18001 ttatgatcgg gggcagactc atacgcccga agtgcaggca tacgcatcgc ccgggtgact
    18061 gcgcctgtaa ataacaaaat atcggcgtga cgcggtgatg aaataacctt aatgccaaaa
    18121 cgttctgcgt cgaatactgg tgtgatggcg gcaaaaattt cgatttcaca ggcgttgcag
    18181 ccgccgcaat cgacgcgata aacgtaagcc gagcgacgga tatcctgtag caaatgctgc
    18241 ttcatctttt gcgtttgctc gtccagcgtg atgggctggc tgacatgttg tgtaagcact
    18301 ggactcatag ctgctcctta gccaccagcg gtacatctgt atcgtcgttg agcagcgtcg
    18361 cgcgttgttt gcactccggg cagacgctcg cttgcgccca taacatttcg cggttttgtg
    18421 gcgcattttg ttgctgtgcc aacaattcag cagccagtgc gacggttttt tgcggggcaa
    18481 acgggcgttc gcaacggctg caacgttgta gatggaacgt cgcgcgggta tagagatcgg
    18541 ctttattggt gacggtcagt tcaaagttat tggtaagctg gatggctctg gtcgggcaca
    18601 cttcttcaca acgtccgcag taaatacaac gcctcagata gagctgccag gtgcgcgaat
    18661 tttgctggtc gtcggtctgg atagtcagcg catttgccgg acaagcacag gcgcaggcac
    18721 cgcaggcaat acattggctg ggcatcaggt ccggtttccc gcgaaagcct gggctgacct
    18781 ccagtggcgc gaagggatat ttcaccgtcg cggttccggc gcgcataata gttttcagta
    18841 acttcagcat cttctgtcct tacttcagcg gcgaacggtt acgatcaata ccgtagcgtt
    18901 cgatctcttt atacggcacg gtttttgact ggcgcttgcg cacatctacc agcgtcacac
    18961 ggtcggtaca ggagtagcag ggatcaaggc taccgataat cagcggtgcg tcagaaacgg
    19021 tattgccgcg cagcatgtaa cgcaacaccg gccagttggc gtaggtggcg gcacggcagc
    19081 gccagcggaa caatttttgg ttatcaccga gcatgctcca gtgcacgtct tcaccgcgtg
    19141 gcgcttcaac aaagcccagc gcgaatgcgt gaggtttata gctaaagcct tcggtcagca
    19201 gtggggtatc cggcatgttg tcgagggcaa attccagcat tgccagcgaa tcaaacgtct
    19261 ctttgacacg gaccatcacg cgggagaaaa catcgccgcc ggtaaaggta aacagtgttt
    19321 ttggaatatt gccgtagtcg gcgtaggggt gatcaaagcg caaatcacgg gcgatttgtc
    19381 ggtcgagaat gccaatgccc tgagtgcgtt gttccatatt cggcgtagca agcagcatct
    19441 ctaccagctc cgacacgtcg gcgcgcatct cgcgcaccag tttcagcgtt tgcagacgtt
    19501 gctctttgag aatatcgcgg cgaacaccac caatcagatt cagaccgtag gttttacgcg
    19561 acccgatcag caattccgcc atcgtcatcg acttttcccg cacgcggaaa aattgcataa
    19621 agccggtatc aaaaccaacg aaatggcagg agaggccaag gttaagcaaa tgactgtgta
    19681 gccgttcgac ttccagcaga atcgagcgaa tagtatgtgc tcgttgcggc acctcaatcc
    19741 ccagtgcatt ttcaaccgag ttggtatagg ccacactgtg ggcaaaaccg caaatcccac
    19801 acacgcggtc cgataagaag gtcacttcgt tgtagcccat ccgcgtttct gccagtttct
    19861 ccatgccgcg atggacataa aacaggcggt aatcagcatc gacaatttgc tcgccatcca
    19921 caaacaagcg gaagtgaccc agttcatcgg aggtgatatg cagcgggccg acagggatca
    19981 cctgcgcatc gctgttgccc tcattgataa acggatacgt ttcggaatca gtcgtcggtt
    20041 cagggcgcag tcgataatcc atcgcatctt tgcgcagcgg atgcatatct tccggccagt
    20101 catcgggcaa caccaggcga cgatgatccg gcaggccaac cggaatcagc ccgtacatat
    20161 cgcgaatttc tcgctcgccc cagaccgcgg cagggacgcg cggtgtgact gacggaaact
    20221 cccgactgtc ggcatcgacc agtgccttca ccacgatcca gcatttttcg gccccttcca
    20281 ttgaaagggc ataataaacc gcgtaatgac cgttaagtgt ccgctcgtcg ttgccaaaca
    20341 ggactggaag ccagccatca tgttgataat aaagataatg tacaacgtca ggcagcagat
    20401 tgattttcac cgtaatggtg acctgttcag gcgtttgtcg ctcttcatcc agcaccgcgc
    20461 cggggaactg cgttttcagg gcggcgagaa tcgcttcgcc acgatttgac gatgaattaa
    20521 cgttcactgt gtttctccga aacagatgcg gtgcctgaag gcaaaaaatc atgccaggtg
    20581 ctacgttgtg caggcaggtc gtgcgtccct gagaggacta tagtggaagc gcccgccagg
    20641 atcctgatga caggttgtgg aatatgcgtt cccatcgcca gcatcatgac cagcagaatc
    20701 accattggcg aggtggtcaa ccagccgaga tcaccccggt taacggcctg cggcggtttc
    20761 gccattaaca cccgcgctgc catccgtacc aggcccgcca gcaccagcgt taacagcaat
    20821 aacagcagga cgataatcag caggtgatta cgtgccagtc cggcggtaat ggtcataaat
    20881 tcgctaagaa aaatgttgaa gggcggcatc cctgccagcg ccagcgcacc gccgccaaac
    20941 agcacggcgg taaatggcat gattttgagc atcccacaga cgacgttgag atcgcgcgtg
    21001 ccgtacttga gcagtacatt gccggaaccg cagaacagca gcgtttttgc cagactgtgg
    21061 tttaaggtgt gcagcagcgc ggcaaaaatt cccagtggcc cgccaatgcc tagcgccacc
    21121 gcgaccagtc ccatgttctc cacgctggag tacgccagca gacgcttaat gtcccgctgt
    21181 accagaatga aaaatgccgc cacggcaacc gacaacatgc cgaagatgag caacaaccgg
    21241 ttggggaaat cgctgccgat ggcctggcag ataatgatgt aatagcgaat tagcaccaac
    21301 agcgcgcagt tcagcaatac ggcggagagc agggcgctga ccggactcgg tgcttcactg
    21361 tgagcatccg gcagccaggc gtgcatggga aatagcccgg ttttggtgcc aaagccaatt
    21421 agcacaaaca caaaggccaa cagcattaat gttgggtcaa gcaaggacga ttgcttaaga
    21481 acctcgctcc agaatatcgc catttctgcc tgcggcataa cgctggcgac gttggcgtat
    21541 accagcacgg taccgaacag accaaaagcg acaccaacag tacaaataat gatgtacttc
    21601 catgctgctt ccagcgatga acgctgaccg taaatgccta ccagaaacgc cgagcttaag
    21661 gtggtggctt cgatcgccgc ccacatcaca atcaggttat tgctggtaac aaccagcagc
    21721 atggtaaaca aaaacagatg gaagaaaccg tagtaatcgc acagcgtaac gggggaaagc
    21781 tcgccgtgtg ccacttcatg acgcatgtag ccaatcgagt aaacaccggt gagaaagcca
    21841 atcacaccaa gaatggcgag gaacaaaccg cccagaccat caatatgaag ccacagtccc
    21901 gcagcgaata tttctcctgc atcagcggca gtttggacca cccagagtgc cagaatcagc
    21961 agcagtgtga tccctaagct atgtaataca gtcaccgtac aagtcgcaga gagcccccgt
    22021 ttccggcagg caaaacagag cagcgaaaaa agcagcggcg tgagcaggag taaagcgaac
    22081 atcacagaat aactcatctc attatccctt cagcgcggtc aagttgttca cgtccagcgt
    22141 gccgtgggta cgccatattt ttcttgccag taacaccatc acaatgacgg cgaagatggc
    22201 gtcggtagcg atacctattt ccaccagttc cggtgctcgc caggcaagaa gcgccagcac
    22261 cagatgggag ccgttttcca tcaggcagta accaaaaatt tgccgcagga tattgcgctg
    22321 gctgacaatg cacagcaggc caagcagaaa atgacctaac gctaccgcca gcgccggttt
    22381 cagcccggta gccatcggta gcttcacggg ctgaacgaca aatgcgcaaa gcaggacaat
    22441 taacgccgcg agcagtgcca tcattgccgg accgaataac gctttttccg ggatgttctg
    22501 gggaatattt cgtgcagcgt aagtcatgat taacggtacc agcagcactt tggtgataaa
    22561 ggcgctggcg gaccagatca gcagttgctc tgcggcgaac aggcacgaga gagtggcgaa
    22621 aatagacacc agcaccagcg actggcaggc gtaaaatccg catgacaggc gatagctttt
    22681 gacgctaatc acaaacagcg atgtcagcat catcagtccc gccagattat ttacgatcat
    22741 agaaccggtc attcatcatc tccttactgc aaccaggtcg cggcgattac gctggaaatc
    22801 agcgacatca caatcagtac aatcaacacc aggcgcattg accctggcag cggtgcggca
    22861 tcggcgacgg cctcgctcgg tttgccaggc acaacgcgac caaaccagcg aataaaccag
    22921 gcgaaactgg cgaccgattc aatcatcagc agaatcatgg cgggcagcag gatccagtac
    22981 tccactgaca acgcaaaacc ggcagcaaac agcgggaatt tactgaagaa gccgttgaac
    23041 ggcggcacgc cggtaatcgc cagcgctgcc acgcagaaac ccacgcctgg caacggcagg
    23101 gtgtgcaata cgccacgcag acgcggcaac aagcgcgtgc cgcagctgta actcagcgca
    23161 cctgctacaa ggaaaaacag gcttttagcg aacgcgtggt tgacgatgta ggcgatgctg
    23221 ccctccagcg ccagccgcga gccgaagatg gagagcgaca aaccgaagaa catccagcca
    23281 agttgagtga tggtcgacca ggccagcaac cgcttcatat cctgctgtgg caaatacatc
    23341 agaaagccat aaagaatggt gaccagcgcc atgaccatgc caacgccgcc aatcacatgc
    23401 gggatattgc cgccgtcgat aatggcgcgg gcaaaaatgt aaacgcccac tttcaccttc
    23461 gatgcggcgt ggagataggc actgatcggt gttggcgctt ccattgcatc cggtagccac
    23521 gcttgcatcg gtagctgggc cgatttcccc cacgcggcaa acaggatgcc gccataaacc
    23581 agataacgtg cgtcgccgtg taactcgctc atcgcgctaa gcgcaaacgt tccggtctgc
    23641 aaaaacagcg tggcggcggc aagatacaac cccaacgagc cgatatgagt gataagtaac
    23701 gcttttagtg ctgaacgctg tgctttatcg ctctggtaat aactgatcaa cgcccaggag
    23761 cagccgcccg taatttcaaa aaacaacaac tgaccgagca gcgtcgagga gagtaccagt
    23821 cccgccatcg cgccgataaa caacagcaaa aatgcgtaat aacgattcgt gccgttatgc
    23881 gggtgttcgc gatttttatc cgtcagataa cccgtggagt agatcgtgac cagcaaaccg
    23941 agaaacacca ccacaaacag aatcagcgta ctcacgcgat caatgaccag gccaaacaat
    24001 gccacatccc ccacgttgac caatgtcaac gtgacggcaa ctttatcggc ctgataaaac
    24061 gcggagatca gtgacaacat gcacagcgtg gtcagcgcgg cgaacaaaac cccccattcg
    24121 gcggcccgac gttgtggcga aaacgaaacg accagtgcgc cgataaaagg cagcaataac
    24181 gtcgtcagag caagattttc catattccgt cagtgctcct tacagaccgg ttaaccagaa
    24241 gacccatgca agcgcagcaa ggctgaagcc aagccaggtc acatggtgaa tgagtaaaaa
    24301 acgcccgcgt gccagcgtgt tttctgcgat tgaggccagt acaaaaatca gcaaaacctt
    24361 gagcagcgta acgacaagtg aagtcagcag gcaggcgaga gaaagttctt gcgcgcgccc
    24421 aaaggacaga aacagggcca caaacagtga tgccatcacg acctgtttca gccccagccc
    24481 ccatttcgct agcgccagcc cggcaccgga atattcggtc agcgggcctt cctgtaattc
    24541 ctgttctgct tcagcaacat caaagggaat ttttcccatc tcaatgaagc aggcaaaacc
    24601 acaggccagt aacgccagta cggtggttag cggcgagttc cagcccatcg ccagcgtatt
    24661 gctgatcatc tcgatatgcg tggaacctgc tatcagcgcc aataccagca gtgagagaat
    24721 aagcattggt tcgaccagaa tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa
    24781 cggacttccg gtatccagcc cggaaagagc aaagaaaaaa cgaaacaggg caagaagata
    24841 gataagggtg atcagatcgc cgccgcccgc aaaaggggaa acggtaataa acagtggtaa
    24901 ggccatcgcc agcaccagca tgctgctgat taatacccac ggcatcaggc ggaacatcag
    24961 acctgaagat gtcggcgcaa cttcctggcg tttaaacagt ttgtggatat cgcgataatc
    25021 ctgccagatc cccggcccgc ggcgggagtg catacgcgcg cgtatctgcc gggaaatacc
    25081 cgtaaaaagt ggggttagca tcagcagaat cacggcctgt gctaacgcaa aaataaccag
    25141 atatccgtcg caaagagttt gtctcatggt gatttcctta gacggcaata gcgattagca
    25201 gcacaaccag tgcggcgacc acatacaggc aatagagacg aaagtcgccg ctttgcagat
    25261 gctgtatttc tttggccagc cgttgggtgg cgctcacgat ggggcggatc acccgctcat
    25321 cccagaaggg ttctgtgctc tgagccctgg cggtgacgtg cgcaaaacct ttatttagcc
    25381 tcagcgtagg gtcgagctgt tgtcgtagac gaaataacgc agagaagacc acacgcagcg
    25441 gctgcatcac gccattgcct gacggggcca tcgcattttc ccagccgtag ccgcatgccc
    25501 aggcatctcc tgtgcgacga aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc
    25561 caggcagcaa aggcatcgcc agtagtaaca gcaacagtaa tgagggggtg acctgtgtat
    25621 gaaacgtgcc gggtacaagt gctattccgc tggcgacagt gacgggaggg gtattggtaa
    25681 acgcatgggc aatatgcatt atcttcggtg ccagccaact ggcactaagc gcaattaata
    25741 cgcagagtgc cgcgagcagt agcatggcga cgatcatcgt atttggcact tcctgtgcct
    25801 cttcagcgtg tgtactgcgc ggcgcaccac agaaagtaat accgtacatt tttacgaagc
    25861 acattactgc cagcccacca gtgactgcca gcattaccat agcaatagga cccgcaagtt
    25921 gtagcgctac ggcttccaca cgacttagtg agaacagcga ctgccaggtg taccattcgc
    25981 tgataaaacc attcagcgga ggaatggctg atatcgcgag gcaaccaatc aggcatgctg
    26041 cggctgtcca cggcatccgt ttcgccagtg cccccatttt ttccatgtcg tgggtatgca
    26101 aacgcgaaat aatcgcgccc gcgccgagaa atagcagccc tttgaacagc gcatggttga
    26161 gcagatgaaa cagtgcgccg agcagtccaa ccacggtgag cagcgggtcg tgcagtgaca
    26221 gaccgaccat cgccacaccg actgccagca aaataatgcc gacgttttcg acggtactcc
    26281 aggccagcag ccgtttgata tcctgttccg ccagcgcata cagcacgccc aggagcgcgg
    26341 agattgcgcc gatcgccatc accagaatgc cccaccacag cggcaaaccc gtttgcgcca
    26401 gcaggtccat cgctactttc aggatgccga aaataccgat tttgaccatt acgccagaca
    26461 tcaacgccga agcgtgcgat ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg
    26521 gcatcatccc ggctttcgcg ccaaaaccga aaaaggccag caggaacacc gccgacgcca
    26581 gccccggaga aagtgaaagc gtgcggaaac tggcaaaatc gaggctgccg ctttcgcgcc
    26641 ccatcagcaa gaaggcgatc ataatcagca ccgatccggc gtgggcgata aaaaagtaga
    26701 gcatgccagc gttgatcgac gttttatcct gcctggcaat gaccagaaac caggaagaca
    26761 gcgacatcat ttcaaacagc acgatgaacc aaaaagcgtt gtccatcacc agcagggcaa
    26821 ccatcgatgc gatgaaaaga ttcataaaga agcccatcgc cgccgcgcct ttgccctcgt
    26881 attcgcgcat ataagtcaat gaatagagcg aacaaaccac caccagcaag gagataacca
    26941 gcaccataaa tgccgagagg ctatccattc gcagggtaaa atcggcaaag tcaaaagggg
    27001 tggcaaaacg ggcgactaaa gttacaccag catgtaattg cgtaattccg ctgatgatgc
    27061 caatcacgcc gcccaccagc gatgtgatgc cggaaagctt aatagccagt ctgtccagac
    27121 cgagtaaaaa cagcgaagcc agactggcaa acagatagag gatcagcgac caggttaata
    27181 attgcagggc atccattagc gttgctcctg agtgagggaa aggggatcct ccccgccatt
    27241 ggcaaaccag cttgcggcaa ggcgctgttt ttctttcatc tgtcgttgca ggctatcgtc
    27301 ggtgatcagc cgtaacgcct gattcgggca agcgcgaacg caggccggac cttctggcaa
    27361 gaaatcacac aggtcgcatt tcaccgcgac ggtctggaca cccggttccc agcgcagcaa
    27421 tgcatgttgg ggcggcacgt tttcttcgcc gtctttgagt gagccttcag cctgaaaaac
    27481 atattgcgca ggggcattca ccggacggct tcctgaagcg ctgattgcgc caaatgggca
    27541 gaccacggcg caaagcttgc agccaataca gaggctttcg ttgagttgga tcgcatcatc
    27601 cctctgggag atggcattaa ccgggcagac ctgcaggcaa ggggcttcct cacagtgatg
    27661 acacacgaca ggggcagtga gtgttgacgt cttcgccagg gccaggcgcg ggtgttgctg
    27721 taaaccttgc gttttatgca cgtccgaaca ggcagcgaga caggtattac atcctgtaca
    27781 ccacagtggt tcggccacca caaagcggtt catgtcggct ccttgaaaat gatatgaaac
    27841 attgcatttc atattgcata aaggatgcca ttctgattaa ctcaatatat tcaataagtt
    27901 atgtattgac tggattctga acggtacaaa aatgatggtc attaacgttc agcaaaaaat
    27961 ggattcgaca cccgtgtcgc aggggaaagc gagaatgtga tcttgatcac gagaagtgag
    28021 gtatgctggc cgcaagcgca gccagcacgg aatggagcaa agtaatcagg cgtgttcttt
    28081 cagccagttc agcacaacgt cgtggtgatt gctggttttg aaatcgtcaa agacatgttc
    28141 gattttgcca tcagcgtcaa tcaggaagct gatgcgatga atgccatcgt aggttttgcc
    28201 catgaaggac ttttcacccc agacgccgaa ttgttcgcac acctggtggt cctcatcaga
    28261 caggagcgta aagttaagca gctctttttc cgcaaaacgg gagagttttt cgggtttatc
    28321 ggtgctgata cccagcacat caacgcccgc ttttttcaac tcatccatgt tatcgcgtaa
    28381 gccgcaggcc tgtacggtac agccgggggt catggctttc gggtagaaat aaaccagaac
    28441 acgctgtccc tggaagtcgg tcaaattaac ttgttctccg tcttgatccg gcaagctaaa
    28501 tttcggtgcg atatcaccgg ctttcagtgg attcattact taactccatc ctgttcatca
    28561 tgttgggaat aattgacgac gttaatactg ccttgtgcat tgagttctgt acatagggct
    28621 ttgaacgctt gctcaatatt tgctgcgtcc gcagatgcgg ggctgtgggc ggttatctga
    28681 atatgcaact gcgcagcccg ttcattttca gcaggttgcg tgcgcgacac cagctccgca
    28741 atgttcatat gatgcgcgtc gaaaagtgct gtgaagcgtt caattaaatg cggggagtct
    28801 gccacatcga cctgaaccca gacagatgct ggcattggcg gacgcggacg cgccgtcgtg
    28861 cgcttcatca cgattaaaag atccagttcg gcacctttca acggtaacgt tgattcaatc
    28921 agagtgatgg cattccatga accggaaagc agcatgataa acgtgaactc ttctcccagc
    28981 atcgccaggc gactgtcttc aatattacag ccgcaactac tgacatgacg ggtgatggtg
    29041 ttcacaattc cagggcgatc ggcacccaac gcagtgatca ccagataatg ttgcgatgac
    29101 agtgtcaaac tggttattcc tttaaggggt gagttgttct taaggaaagc ataaaaaaaa
    29161 catgcataca acaatcagaa cggttctgtc tgcttgcttt taatgccata ccaaacgtac
    29221 cattgagaca cttgtttgca cagaggatgg cccatgttca cgggaagtat tgtcgcgatt
    29281 gttactccga tggatgaaaa aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat
    29341 tatcatgtcg ccagcggtac ttcggcgatc gtttctgttg gcaccactgg cgagtccgct
    29401 accttaaatc atgacgaaca tgctgatgtg gtgatgatga cgctggatct ggctgatggg
    29461 cgcattccgg taattgccgg gaccggcgct aacgctactg cggaagccat tagcctgacg
    29521 cagcgcttca atgacagtgg tatcgtcggc tgcctgacgg taacccctta ctacaatcgt
    29581 ccgtcgcaag aaggtttgta tcagcatttc aaagccatcg ctgagcatac tgacctgccg
    29641 caaattctgt ataatgtgcc gtcccgtact ggctgcgatc tgctcccgga aacggtgggc
    29701 cgtctggcga aagtaaaaaa tattatcgga atcaaagagg caacagggaa cttaacgcgt
    29761 gtaaaccaga tcaaagagct ggtttcagat gattttgttc tgctgagcgg cgatgatgcg
    29821 agcgcgctgg acttcatgca attgggcggt catggggtta tttccgttac ggctaacgtc
    29881 gcagcgcgtg atatggccca gatgtgcaaa ctggcagcag aagggcattt tgccgaggca
    29941 cgcgttatta atcagcgtct gatgccatta cacaacaaac tatttgtcga acccaatcca
    30001 atcccggtga aatgggcatg taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg
    30061 ccaatgacac caatcaccga cagtggtcgt gagacggtca gagcggcgct taagcatgcc
    30121 ggtttgctgt aaagtttagg gagatttgat ggcttactct gttcaaaagt cgcgcctggc
    30181 aaaggttgcg ggtgtttcgc ttgttttatt actcgctgcc tgtagttctg actcacgcta
    30241 taagcgtcag gtcagtggtg atgaagccta cctggaagcg gcaccgcttg cggagcttca
    30301 tgccccggct ggaatgattt tgccggtgac ctccggtgat tatgcaatcc cggtgaccaa
    30361 cggtagtggt gctgtcggta aggcgctgga cattcgtcca ccagcccagc cgctggcact
    30421 ggtttctggc gcgcgtaccc agttcacggg cgataccgct tcattgctgg tggaaaatgg
    30481 tcgtggcaat actctgtggc cgcaggtggt tagcgtgctg caggcgaaaa actacaccat
    30541 cacccaacgt gatgatgctg gtcagacact gaccaccgat tgggtacaat ggaaccgtct
    30601 ggacgaagac gagcagtatc gtggtcgtta tcaaatctct gttaagccgc agggttatca
    30661 gcaggcggtt acggttaaac tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc
    30721 ggcttccatg cagcgttaca gcacggagat gatgaacgtt atttccgccg gtctggataa
    30781 atctgccact gacgccgcga acgctgcgca aaatcgtgcc tccaccacta tggacgtaca
    30841 aagtgcagct gatgacaccg gtttaccaat gctggtcgta cgcgggccgt tcaatgtggt
    30901 ttggcaacgt ctgccagcgg cgctggaaaa agtgggcatg aaagtgaccg acagcacccg
    30961 ttcgcagggc aacatggccg taacttataa gccgctgtct gacagcgact ggcaggaact
    31021 gggcgcgagc gatccaggcc tggcatccgg tgactataaa ctgcaggttg gcgatttaga
    31081 taaccgcagc agcctacagt tcatcgatcc gaaaggtcat actctgactc agagtcagaa
    31141 cgacgcgctg gtagctgtct tccaggctgc gtttagcaag taaaaataca gggctggaat
    31201 catccggccc ttttttctga tatgatacgc aaacgtgtgc gtcggcagga aaacgcgatt
    31261 ttagcggtaa ttcgcacgaa atttgtttgt cggacgtagt tcggataagg cgttcacgcc
    31321 gcatccgaca aaacatccgg cacaccagac agcaaaagat tttaaaacgt taattcacac
    31381 ccaggagtga taaagatgca aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta
    31441 tacagcacgg aaaacccgga cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg
    31501 gatggcgcgc gcattgagca gtttgatcgc aaaggtatgg tgaacaacaa gttcaattac
    31561 ttcattatga gcaaactggc tgaagcgggt atcccgactc aaatggagcg tctgctctcc
    31621 gataccgaat gtctggtgaa aaagctggat atggtgccgg ttgagtgtgt cgtgcgtaac
    31681 cgtgctgctg gctctctggt gaaacgtctt ggaatcgaag aaggtattga gctgaacccg
    31741 ccgctgttcg atctgttcct gaaaaacgac gccatgcacg atccgatggt caacgaatct
    31801 tactgcgaaa cctttggctg ggtgagcaaa gagaacctgg cgcgtatgaa agagctgacc
    31861 tacaaagcga acgacgtgct gaaaaaactg ttcgatgatg ctggtctgat tctggtcgac
    31921 ttcaagctgg agtttggcct gtacaaaggt gaagtggtac tgggcgatga gttctccccg
    31981 gacggcagcc gcctgtggga caaagaaaca ctggagaaaa tggacaaaga ccgtttccgc
    32041 cagagcctcg gtggcctgat cgaagcctat gaagccgtcg cccgccgcct gggtgtacag
    32101 ctggactgat ttttctggtt gcttgtttct ttagccggag ggtactccgg ctaaatgtct
    32161 ttgatacccc cgctacaata cttctcatta tctccctttg acaggaaagt cataccaggc
    32221 caatatgtat atacataacg tatatcccaa aaaggggggg ctatggaaac tactgtattt
    32281 ttaagtaatc gcagtcaggc cgtcagatta ccgaaagcgg tcgctctgcc agaagacgtt
    32341 aagaaagtag aaattattgc gattggtcgt acaagaatca tcactcccgc cggtgagtcc
    32401 tgggacagtt ggtttgatgg cgaaaatgtg agtgcggatt ttatggatat ccgtgatcaa
    32461 cccgcaatgc aggaaaggga atcattttga tgctgaaatt catgctgga
//
LOCUS       DAZFRJ010000033        32122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000033 DAZFRJ010000000
VERSION     DAZFRJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.033"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(153..620)
                     /gene="yiaL"
                     /locus_tag="ACHKUN_001411"
     CDS             complement(153..620)
                     /gene="yiaL"
                     /locus_tag="ACHKUN_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO6659703.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            complement(632..1630)
                     /gene="yiaK"
                     /locus_tag="ACHKUN_001412"
     CDS             complement(632..1630)
                     /gene="yiaK"
                     /locus_tag="ACHKUN_001412"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO6659704.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1831..2679
                     /gene="yiaJ"
                     /locus_tag="ACHKUN_001413"
     CDS             1831..2679
                     /gene="yiaJ"
                     /locus_tag="ACHKUN_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO6659705.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            2781..3254
                     /gene="ysaA"
                     /locus_tag="ACHKUN_001414"
     CDS             2781..3254
                     /gene="ysaA"
                     /locus_tag="ACHKUN_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6659706.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHCWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(3406..4659)
                     /gene="avtA"
                     /locus_tag="ACHKUN_001415"
     CDS             complement(3406..4659)
                     /gene="avtA"
                     /locus_tag="ACHKUN_001415"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO6659707.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4837..6867)
                     /gene="malS"
                     /locus_tag="ACHKUN_001416"
     CDS             complement(4837..6867)
                     /gene="malS"
                     /locus_tag="ACHKUN_001416"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6659708.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            7187..8011
                     /gene="bax"
                     /locus_tag="ACHKUN_001417"
     CDS             7187..8011
                     /gene="bax"
                     /locus_tag="ACHKUN_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO6659709.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(8119..9297)
                     /gene="xylR"
                     /locus_tag="ACHKUN_001418"
     CDS             complement(8119..9297)
                     /gene="xylR"
                     /locus_tag="ACHKUN_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO6659710.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(9375..10556)
                     /gene="xylH"
                     /locus_tag="ACHKUN_001419"
     CDS             complement(9375..10556)
                     /gene="xylH"
                     /locus_tag="ACHKUN_001419"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO6659711.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(10534..12075)
                     /gene="xylG"
                     /locus_tag="ACHKUN_001420"
     CDS             complement(10534..12075)
                     /gene="xylG"
                     /locus_tag="ACHKUN_001420"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO6659712.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(12153..13145)
                     /gene="xylF"
                     /locus_tag="ACHKUN_001421"
     CDS             complement(12153..13145)
                     /gene="xylF"
                     /locus_tag="ACHKUN_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6659713.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            13511..14833
                     /gene="xylA"
                     /locus_tag="ACHKUN_001422"
     CDS             13511..14833
                     /gene="xylA"
                     /locus_tag="ACHKUN_001422"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO6659714.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            14905..16359
                     /gene="xylB"
                     /locus_tag="ACHKUN_001423"
     CDS             14905..16359
                     /gene="xylB"
                     /locus_tag="ACHKUN_001423"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO6659715.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            16528..16869
                     /gene="yiaB"
                     /locus_tag="ACHKUN_001424"
     CDS             16528..16869
                     /gene="yiaB"
                     /locus_tag="ACHKUN_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO6659716.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            16915..17352
                     /gene="yiaA"
                     /locus_tag="ACHKUN_001425"
     CDS             16915..17352
                     /gene="yiaA"
                     /locus_tag="ACHKUN_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO6659717.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(17394..18389)
                     /gene="wecH"
                     /locus_tag="ACHKUN_001426"
     CDS             complement(17394..18389)
                     /gene="wecH"
                     /locus_tag="ACHKUN_001426"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO6659718.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            18564..18863
                     /gene="ysaB"
                     /locus_tag="ACHKUN_001427"
     CDS             18564..18863
                     /gene="ysaB"
                     /locus_tag="ACHKUN_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO6659719.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            18958..19869
                     /gene="glyQ"
                     /locus_tag="ACHKUN_001428"
     CDS             18958..19869
                     /gene="glyQ"
                     /locus_tag="ACHKUN_001428"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO6659720.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            19879..21948
                     /gene="glyS"
                     /locus_tag="ACHKUN_001429"
     CDS             19879..21948
                     /gene="glyS"
                     /locus_tag="ACHKUN_001429"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO6659721.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            22273..22425
                     /gene="hokA"
                     /locus_tag="ACHKUN_001430"
     CDS             22273..22425
                     /gene="hokA"
                     /locus_tag="ACHKUN_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO6659722.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(22414..22473)
                     /locus_tag="ACHKUN_001431"
     CDS             complement(22414..22473)
                     /locus_tag="ACHKUN_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659723.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(22613..22825)
                     /gene="cspA"
                     /locus_tag="ACHKUN_001432"
     CDS             complement(22613..22825)
                     /gene="cspA"
                     /locus_tag="ACHKUN_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO6659724.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(23106..23396)
                     /gene="yiaG"
                     /locus_tag="ACHKUN_001433"
     CDS             complement(23106..23396)
                     /gene="yiaG"
                     /locus_tag="ACHKUN_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO6659725.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            23830..24540
                     /gene="yiaF"
                     /locus_tag="ACHKUN_001434"
     CDS             23830..24540
                     /gene="yiaF"
                     /locus_tag="ACHKUN_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO6659726.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(24590..25564)
                     /gene="ghrB"
                     /locus_tag="ACHKUN_001435"
     CDS             complement(24590..25564)
                     /gene="ghrB"
                     /locus_tag="ACHKUN_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO6659727.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(25668..26327)
                     /gene="yiaD"
                     /locus_tag="ACHKUN_001436"
     CDS             complement(25668..26327)
                     /gene="yiaD"
                     /locus_tag="ACHKUN_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO6659728.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            26480..28813
                     /gene="bisC"
                     /locus_tag="ACHKUN_001437"
     CDS             26480..28813
                     /gene="bisC"
                     /locus_tag="ACHKUN_001437"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO6659729.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(28782..29222)
                     /gene="yiaC"
                     /locus_tag="ACHKUN_001438"
     CDS             complement(28782..29222)
                     /gene="yiaC"
                     /locus_tag="ACHKUN_001438"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6659730.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(29219..29782)
                     /gene="tag"
                     /locus_tag="ACHKUN_001439"
     CDS             complement(29219..29782)
                     /gene="tag"
                     /locus_tag="ACHKUN_001439"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO6659731.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            29940..30638
                     /gene="yhjY"
                     /locus_tag="ACHKUN_001440"
     CDS             29940..30638
                     /gene="yhjY"
                     /locus_tag="ACHKUN_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6659732.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            30867..32069
                     /gene="yhjX"
                     /locus_tag="ACHKUN_001441"
     CDS             30867..32069
                     /gene="yhjX"
                     /locus_tag="ACHKUN_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6659733.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         7887 a         7851 c         8182 g         8202 t
ORIGIN      
        1 ttaatcgcca gtattgcttc aagtattttt ttcatagcta ttccttgagg ctatgtctgt
       61 cataattcaa gttgcatgtg cagcaaccta aattattaat atagttagag caataacatt
      121 acccagtcgt tctggcgtag acgattattc gattaattca gcgccgttaa tgcgacttta
      181 accacaattt ttcgtatctc agaggctgtt tgcaaaatac aacctggtcg gtgaacatcc
      241 tgcggaaaga aaattgcgta gctgcccggt atcatttcta taaatgattc atgttcactg
      301 tcatgataaa aaataatatc gcgctgctct aatagtgatt cgctgacttt attatttccc
      361 gtatcaatag caatgccgat tttctcttca ccccacgcca gaaactgaat atcgatatac
      421 cgacgatgca cttccggacg gttttccacc gcttcgcgtg tggttaaatc gataatttgc
      481 gcataaatat ttttgccgtc gatttcgaca acgcccggct ccagggcgtt gaaatcggta
      541 gcgcgcagaa aatcgagcgc cttttcaatg gcggcgggca aacggcacgg attgggctgc
      601 gcgatatgtc caaaaatcat gacttatctc cttataacgc ctggattttg gcccacacgc
      661 tgtcatcaac ggtaataccg ttacggcggt tttcggccag cagggtagta aattcgtggc
      721 cgggtaagcg gatggcctgg ttttcgtcag cgcgttcagc ggtagtaacg taatccatga
      781 tacgttgcag cttggcatcg cgggtgggac cgtcgataag cttgtccact tcaatggcaa
      841 taaaaatttg tgaaatgccg tattcgtcgc tgttgtcctg ggtgacttcg gcaaccgatg
      901 cgccgtcgga aaggagagta gcgatcatat ccagcacaat cgacatgcca gaacctttcc
      961 agtagcccat cggcaaaatg cggcgattct tctcgataac gccaggttct ttggtcaaat
     1021 tgccctcatc atcaaagcca ccatcgaccg ggagctgacg gcccgccagg cggttaactt
     1081 ctaacatgcc gtaagagaac atcgacatcg acatatcgac catggtgatc ggcgtggaag
     1141 gaatggcgac gatcagcggg ttagtgccaa tgcgacactc ttttgcgccc cacggcggca
     1201 ttacggcgat ggagttggtc cagcaaatgc caatatagcc tttttccgcc gcctgccagc
     1261 cgtagctgcc gccgcgcatc cagtggttgg cattacgaag tgccaccaga ccaataccgt
     1321 gatcggcagc cagttcaatg gcgcgatcca tcatcttttt cgccgtcagg ttgccgatcg
     1381 aacgctgggc atcccactgt tcaattgcgc cgaggctggt tatacgtttg ggttgggcat
     1441 caggaatgat atcgccgttt tccagttgtt gaatgaaacg agggaaacga ttaacgccgt
     1501 gagaataaac gccggattcg gtggtgcggg cgaacatctc tgcacaggcg tcagccgttt
     1561 cgctgtcaac gccgcgtgaa attaagaccc gattaaaggc tgcttttaac tgctcaaatg
     1621 tcactttcat cccaggcttc cttgtttttt tgatcgcttt tttggcttaa aatttcaata
     1681 tgcgaaactt gatttcaaat atatcgatca ctttttaaca aagcaatctg attgatgaat
     1741 ttcaaaagac ataaaaatca agtggttata aattatctgt cgagatcgtg aactacggca
     1801 cactttgcgc taccatcagg acgcgacaaa atggggaaag aagtgatggg aaaaaaagag
     1861 aacgagatgg cgcaggaaaa agagcgtcca gccggaagcc agagtctgtt tcgcgggttg
     1921 atgctgattg agattttgag caactatcca aacggttgtc cgttggcgca tctttcggag
     1981 ctggctggtt taaataagag taccgtccat cgcttattgc agggattaca gtcctgcggc
     2041 tatgtgacca ccgcgcccgc cgcagggagt taccgcctga ctaccaaatt tattgccgtc
     2101 gggcagaagg cgctgtcttc gctgaatatc attcatatcg ccgctccgca tcttgaggca
     2161 ctgaacatcg ccactggtga aaccattaac ttctccagcc gcgaagacga tcacgctatt
     2221 ttgatttata agctggaacc cacaaccggg atgctgcgaa cccgtgccta tattggccag
     2281 catatgccgc tctactgttc cgcaatgggc aagatctaca tggcgtttgg tcacccggac
     2341 tacgtgaagt catactggga aaaccatcag catgagatcc agccgttaac ccgcaatacc
     2401 attaccgagc tgcccgcgat gttcgacgaa ctggcgcaca ttcgtgaaag cggagcggcg
     2461 atggacagag aagaaaacga actcggcgtc tcctgtattg ctgttccggt gtttgatatt
     2521 catgggcggg tgccgtacgc cgtgtcgatt tcgctttcga catcacgtct gaaacaggtg
     2581 ggagagaaaa atctcctgaa accactgcgt gaaaccgcgc aggctatttc taatgaactg
     2641 ggatttactg tcagggatga cctgggcgcg atcacataac gctttggaca agtgccaaaa
     2701 ctttaacatt tccttcgttg gatcaaagca gtagggacgc gctctctggc actctgctgt
     2761 tttagtgcaa aggagtgatc atgaaccggt ttattattgc ggatgcgacg aaatgtatcg
     2821 gttgccgtac ctgtgaagtg gcttgcgcag tgtcgcatca ggagaatcag gattgcgctg
     2881 cgttgtcacc agacgagttt atttcccgta ttcgtgtcat taaagaccac tgctggacca
     2941 cggcagtagc ctgtcatcag tgtgaagatg caccgtgcgc gaatgtctgc cctgttgacg
     3001 cgataagccg cgaacatggg catattttcg ttgaacaaac acgttgcatt ggctgtaaaa
     3061 gctgtatgct ggcttgcccg tttggtgcga tggaggtcgt ttcttcgcgc aaaaaggcga
     3121 gggcgattaa gtgcgatctg tgctggcatc gggagacggg accggcctgt gttgaagcct
     3181 gcccgacaaa ggcgttgcag tgcatggatg tcgagaaagt gcagcggcat cggctacggc
     3241 agcagcctgt ttgaaacgtt tttccggcgt ttctcaacgc cggaatagtg cgctttgttt
     3301 atgccagatg cggcgtaaac gccttatccg gcctacaaat tcttccaaat tcaatatatt
     3361 gcagaaatca tgtaggcctg ataagcgtag cgcatcaggc aatttttagt gactttcagc
     3421 ccaggctctt tctatctctt ccgccagaat cttcaccccc gcctcaattt tctccggctc
     3481 tggtacgtag ttcatgcgca tacattgatg cgtatgcggc cacggtttat ccagccctgg
     3541 gaagaagttg tgccccggca ccatcagcac gccgcgtgct ttcaggcgct gatagagctg
     3601 ctcggtcgta atgggcaaat ccttaaacca tagccagagg aaaatggctc cttccggttt
     3661 atgaatcagg cagcgatttt ccggtaaata gcggcgaatg atggcgatag tttcctgaac
     3721 acgctggtag taaaacggtt tgatgactgt ttcagacagg cgcagcagat cgttacgctt
     3781 aatcatttca cacatcatcg ccggaccaat accgccaggt gccaggctga taatgccgtt
     3841 catattggtg atggcggtga tgattttttc attggcgatg ataatgccgc agcgggagcc
     3901 aggcagcccc agcttggaaa gactcatgca cagcacgata ttcggattcc atagcgggcg
     3961 cgcttcactg aagatgatac ccgggaacgg gacgccataa gcgttatcaa tcaccagcgg
     4021 aatgccgtgt tgattcgcca gcgcgtcaag cttcagcaac tcttcgtcag taatcacatt
     4081 gcctgttgga ttcgtcggcc gggagacgca aatcatcccg gtttcttcgc caatatgcag
     4141 atgctcaaaa tcgacgtggt atttaaactg gccttccggc agcagttcaa tattcggacg
     4201 cgcagagaca aacagatctt cttccagtcc ggcgtcagcg tagccaatgt attccggtgc
     4261 cagcgggaac agcacttttt tgacccgacc atcggcacgg cgtccggcaa acagattaaa
     4321 taagtagaaa aacgcgctct ggctgccgtt tgttagtgca atattctgtg gttcgatatc
     4381 ccaacccaac ttctcgcgca gcattccggc aagcagtgtg agtagctccg ttttcccctg
     4441 tggaccgtcg taattgcaca gtgcatcagt cgctttgcca ctttccagca tgtcggtcag
     4501 tagcgtctgg aagtagtcct gcatttccgg gatctgcgcc ggattaccgc cgccgagcat
     4561 aatcgcgcca ggggtgcgta aaccgtcgtt cagatcttcc atcagcagcg taatgccgga
     4621 gtggcgggta aatttgtcac caaaaaggga gaatgtcata gcgggatatc tgtcgaactc
     4681 tgtagcaagg aaggtaacaa taacgctaca tttgctgggg tgcaaatcgg tctgttgaag
     4741 agtgagcggt gctttatcct gcaacgctga ttagggctga cattttatcc tggttggcgg
     4801 cttcccgagg tactgacgaa cgggaagccg gaaaagtcac tgttgcccag cccagatgac
     4861 cagcacttta tcgtcgccat gctcacgaac aaagccgtag ccctgcttca gcgaaagtgt
     4921 cgtttgtttg cccgcgccaa ttgcgggatg gcgggcgcgg aactggctga ttttctgcca
     4981 gtgcgcgacg ttggcggcag atttaccgct aacatcctgc cagttcatat ccgaacgtgt
     5041 accttgcagc ggatcagaac ctgtaggacc gaacggacgc gaggattcat cgccataaaa
     5101 gatttgtacc gcgcctggcg ctaatagtaa taactctgct gctttgtcgc ccccttcacg
     5161 gaacagacgg gtatcatgcg acgagaggta gctcaacacg ttgaaaccct gcaatttctc
     5221 cgccatttgc tgccaggtca tatccatctg cgcaatacaa tcgacagctt tcgccgcctg
     5281 ctcctgataa tcgaaattga tcatcgcatc gaagccgtgg cgatagtagt cactttgcat
     5341 cacgccgtgg ccccaggctt caccggtcat ccagaaaggt ttgtcatcta atgctttgtc
     5401 ggggttagct tttttccatt cgcgaagcgc ggcgctggct tcggttttca gttgctgcca
     5461 ggcgggcaac tcaacatgtt tggcggtatc gacccgaaaa ccatcaatcc catagtcgcg
     5521 gacccactga cttaaccagt gggttaagta atcgcgaggg gtaaagccgt cgatggcttt
     5581 agcgtgggta tccgttttgt ttttatagaa caccggcaga ccagaagcgg tagttgattc
     5641 ggttttgata tccggcaaaa aggctagcga catggtgaga tcgtcgaatc caggattgtc
     5701 gtaatcgccg atatcggtac ggatccagtt ttttccccac catttatccc agcctgtttt
     5761 gtcgctgaaa ttaatgtaat cgttaaagct atgccaggtt tgcccggcgg caggtttcca
     5821 gtcgctccag cgttcaccca gcgttttttt cacctcgtca ccagaaagat ataacgcgcc
     5881 aaactgatac tcctgcatat ccgccagcgt ggcatagccg gtgtggttca tcacgacatc
     5941 aaagagaata cgaataccgc gctgatgtgc gctatcaacc agcgtccgta gatcggcttc
     6001 gttgcccata ttggcatcaa gattcgtcca gtcctgtgtg taataaccgt ggtaggcata
     6061 atgcgggaaa tcgccttttg taccgccgcc gacccagccg tgaatttgct caaatggggc
     6121 gcttatccat aaagcattaa cgcccaactg ctggaggtaa tccagtttgt tggtcaggcc
     6181 gcgtaaatcg ccgccgtgaa aagtgccaat ttccgccata ccgtctttat gacgtccgta
     6241 actctggtca ttactgggat cgccgttttc gaaacgatct gtcagcacaa agtaaaccgt
     6301 ggcgttatgc cagtcgaaag gggcagaggc gtcagtttct gcccgttcca gcaggagtaa
     6361 accgttgctg gtggcagcgg gttgtaacgt tatttgaccg ttcttcacta tcgcaatttg
     6421 ctggctgtaa taatcccgta cggcggctcc ttccgggaaa gtggcgctga catccagcgt
     6481 gagcggtaat ccatcccatt tcgggcatac acggacctgg cttgctaccg gttcggcggc
     6541 gttctggatg gaaatcatca atgttggcgt gccggagcgg gtgtctattt gcagcgtata
     6601 ttcgccgtcc ctgaacaatc gccattgagg ctgcgtgttg ctacaaggtt gcagggaaag
     6661 catctgattg agttttatcg catccgcagg ctgccagcac tgttggtcaa aatttagcgt
     6721 tagtggacgc gtacctttgg gcaactgcgc gtggctgaca aatgtttccg ttccctgttc
     6781 actaaaggca gggaaccccg gagaagtcca gctggcggca acggcgaagc caggaaggag
     6841 tgtcagaaaa caggcggcga gtttcatggc ggatgagtcc ttattgctgc gatttcagac
     6901 agtttgccag cgataagcca aacaaaactc atccctgagc ggtaagtcca caggatgaga
     6961 agcaagggtg agcgatctcg cgcaaaaaac ggctgaattt tgcgataaac cccacatttt
     7021 ctgcgattta gcgccaatct gaatcgttaa ctcgtgacat agtttcagat ttggactatt
     7081 ccttggggtg ctcttgacag ctatttttga tatgatttga gattcaactc tcaaatttgt
     7141 gaaaaatata aggtgttgga atgattaaat ccgaccagga gacctgatga ttttgactcc
     7201 catacgacga tatggggcga tgattcttat gttactcact ctggtgtttt cgagtgaggt
     7261 gttagcgaag acgcacacaa caacagcgag tcaaaagtcc cacttaacta aagctagtaa
     7321 taaacaggta agcagtaaac aagagtattc tcgcaatagt gcaaagagta attcacttcc
     7381 tgatttgcga aaataccctt ccggaacacc aaggaaaaag gcgtttctcc ggaccgtaat
     7441 gccttacatt accagccaaa atgcagccat tactgcggaa cgtaactggc tgatttcaaa
     7501 acagtatcag ggccaatggt cacctgctga gcgtgcgcgt ctgaaagaca tcgccaaacg
     7561 ctacaaggtg aagtggtctg gtaatacgcg aaaaatcccg tggaatacct tgcttgaacg
     7621 cgtagacatt atccccacca gtatggtggc gacgatggct gcagcagaaa gcggttgggg
     7681 aacgtcaaag ctggcgcgca acaacaacaa tctgttcggc atgaaatgca tgaaaggacg
     7741 ttgtaccaat gcgccgggta aagtgaaagg gtactcacag tttagttctg tcaaagaatc
     7801 ggtgagcgcc tatgtcacta acctcaatac gcatccggct tactcttcat tccgtaaatc
     7861 gcgtgcgcag ctgcgtaaag cggatcagga agtgactgcc acagcgatga ttcacaagct
     7921 gaagggctac tcgaccaaag ggaagagtta taacaactac ctgttcgcaa tgtaccagga
     7981 taaccaacgg ttaatcgcgg cgcatatgta attgcatttc cttgagcctt atctgatttg
     8041 tcggtcggat aaggcttttg ctcgcatcag gtggctgtgc tgagttccct gatgtgacgc
     8101 cgacaattct catcatcgct acaacatgac ctcgctattt acatcgcgat actcttttgg
     8161 cgttgtgtca tatgcttttt taaaaacaga gtagaaatat tgcagcgatg gataaccgca
     8221 catttgcgat atctcattga tcgacaaggt ggttgaaatc agcagactgc gcgctttctc
     8281 cagcttctcg gcatgaatca ttgcatggat ggtttcaccc acctcttctt taaaacgctt
     8341 ctcaagattg gagcgcgaga tcccgaccgc atccagtacc tgatccactt taatcccttt
     8401 acaggcgtga ttacgaatgt aatgcatggc ctgaataacg gcgggatcgg tcagcgagcg
     8461 ataatctgtt gagcgccgtt caatgacgcg aactggtggg accaaaattc gctgtagcgg
     8521 catttcttct ttatctaata atcgatgcaa cagttttgcc gcctgatagc ccatttgccg
     8581 cgcgccctga gcgaccgaag aaagggcgac acgcgacaga tagcgggtca gttcttcgtt
     8641 atcgatgcca atcacgcata atttttccgg tacgggaata tgtagatgtt cacatacttg
     8701 cagaatatgc cgcgcccggg cgtcagtaac ggcaataatc ccggtttgcg gtggcagcgt
     8761 ttgtagccag tctgccagcc gattttgcgc atgttgccag ttctctggcg cggtttctaa
     8821 cccctgataa accactccgc gatacttttc ttcggcgaca agctgacgaa atgcatattc
     8881 gcgttcagtg gcccaacgtt tgccgcttga ttccggaaga ccataaaaag caaagcggtt
     8941 aacgcctttc tcttttaaat gcaaaaatgc gctttcaacc agcgcatagt tatcggtggc
     9001 aatgtaatga acgggtgggt aactttctgc aaggtgatac gagccgccaa ccccaacaat
     9061 ggggacgtcg acatcagcca gcgcttgctc gatctgtttg tcgtcgaagt cggcaatgac
     9121 gccatctcct aaccagtcct tgattttatc aatgcgggcg cggaaatctt cttcaatgaa
     9181 aatatcccat tctgattgtg atgcctgtaa atattcccct acgccttcta ctacctgccg
     9241 gtcataggct ttattggcat tgaacagtaa tgtgatgcgg tgacgtttag taaacatggt
     9301 tcttttcctg ctgaatcatg caaaaactca aaaccggtaa tacgtaaccg gctttgagaa
     9361 aatttttatc aaaatcaaga acggcgtttg gttgcggagt ccatccatac tgccagcaac
     9421 agaatcgcac ctttaacgat atactgccag aaggtcggta catccatcat actcatgccg
     9481 ttatccagtg aagccatgat aaatgccccc attactgctc cggcaacgct tcccacaccg
     9541 ccagccaggc tggtgccgcc aatcacgcat gctgcaattg cgtccagttc ggcgatattt
     9601 cccgcagaag gtgaaccagc gccaagtcga gaactaagga ttaatccggc gatggctacc
     9661 attaatccgt taatcgcgaa cacggcaagt ttggtgcgtt caacgttaat cccggagaga
     9721 cgtgctgctt ccagattgcc gccgatggca taaatgcgtc gtccaaatgc cgtccgcgtt
     9781 gccataaaca ttccgccgag taacagcaat gtcagcagca gaacaggagt gggaacgcca
     9841 cggtaatcat tcaacagcca gattgcgcct aatacgatga tagcggttaa agcctggcga
     9901 ccgactactg cggtagaggc cggagactgc aaacccaaag cctgacggcg cattcttccg
     9961 cgccattgcc aaccaacaaa agccattaag ccaagcgcgc caatgatgaa gccagtgctg
    10021 gcaggtagat agctttgccc aatttgtgac atcgcggcgc tggtggggga aacagtcgtg
    10081 ccgttggtga tgccaatgag tatgccgcga aatgccaaca tgcccgcgag ggtgacaata
    10141 aatgaaggga ctttgcggta cgcgacccac catccgttcc aggcaccgag aagcagtccc
    10201 agaaccaacg tcacaatgat ggtaagtggc aaaggccagc ctaaccagac gtcacaaatc
    10261 gccgcgacgc cacctaatag ccccatcatt gagccgacgg aaaggtcgat ttcagcagaa
    10321 attatgacga acaccattcc taccgcgagg atgccggtaa tcgcggtctg gcgtaacagg
    10381 ttggagacgt tacgggcgct taagtaggca ccatcggtgg tccaggtaaa gaacagcatg
    10441 attgcgatga tagctgcaat catcacgaag acctgcaaat tcagtgattt cagcccggaa
    10501 aagccaccgg atgtcggtac ggccaatttc acttcagacg gattgctttt cgacatgatg
    10561 ttcgctcctc aatgcggctt ccatcacctg ctcctgagtc aggttatgat ttatcaggtt
    10621 ggcttttagt ttcccttcat gcatcaccag tacccgatcg ctaaggccga gcacttcagg
    10681 taattcggaa gagatgacaa taacggcaat accctgctgg acgagttggt taattaattt
    10741 gtagatctcg tatttcgcgc caatatcgat acccctggta ggttcatcaa gaatgaggat
    10801 gcgcgggtta agtaacagac agcgagcgag gatcgctttt tgctgattgc cgccgctcaa
    10861 acgtccaata gcaagatcgg gggacgacgt tttaactttg agttgctgaa ttgattccag
    10921 aatacatttt tgctcttccg cgtcatcaag ctggctaatg ccaccggtaa atttattgag
    10981 tgcggcgagg gtaatatttt taccaaccgc cattaccgga acgatgccgt cgcgctttct
    11041 gtcttcgggt accatcgcaa tcccctgggc gatggcttgc tgacagttac gaatatctac
    11101 ctgtttgcca tcaatataaa tttttccttc ccattgtccg ggccacacgc caaacaggca
    11161 ctgaatggtc tcggtacgtc cggcaccaac gagtccggca atacccagta tttcgccacg
    11221 tttcagggaa aacgagacat cattaactcg tttaatatga cgattgaccg gatgccatgc
    11281 cgtcagatgt tcaatacgta atatttcatc tccggtggta tgtggttcat tagggtaaag
    11341 cgcggttaac tctcgcccga ccatcatggt gataatatcg tcttcactca ttccggcagc
    11401 atcacgcgta ccaatgtgct gtccgtcgcg aataacgcaa atcgtatcgg aaatcgcttt
    11461 gacttcgttg agtttgtgcg aaatataaat acaggcgata ccgtgctgtt gtagatcgcg
    11521 aataatatcc agtaaaaccg acgtttcctg ctcagttaat gaggctgtcg gttcatcgag
    11581 aattaacaag cgcacctgtt tattaagtgc cttggcaatt tcaaccagtt gttgttgccc
    11641 aagccctaaa tcgccaacgc gggtatcagg tgaaatggat aaactgacct gtgcgagcag
    11701 cttctgacag cgtagcgtca tcaggtcata atccataatg ccattgtggg ttatttcgtt
    11761 acccaggaag atattttcca gcacggtcaa ttctttcacc agggccaatt cctggtgaat
    11821 gatggcgata cctttgcgtt cggtatcgcg gatgtgactc gcctgaatct cttctcccgc
    11881 aaaaataatt tcgccttcgt aggagccatg gggataaata ccacacagca ctttcatcag
    11941 cgttgattta ccagacccat tttccccaca aagtgagacg atttcgccag cattcaaccg
    12001 caagcagacg ttatcaatcg ccttcacact gccgaaggtt ttggtaatat tcttcatttc
    12061 aagtagataa ggcataacga ctccacctaa gccaattcat tcacgcggca tggagagaaa
    12121 tcacgccccc gctccgcgcc ggggcgtaac gcttacagct cgctctcttt gtggaatccg
    12181 tctttaacta ccgtgtcttt gatgttgttt ttattcacat cgatcggtgt caggaggcgg
    12241 gagggcacat ctttcaggcc attattcagt gtggtatctg cttttggttc ctgaccattg
    12301 cccaactcaa cggcaatttc tgcggcagta tttgccaaca acgtaatagg tttatacacc
    12361 gtcatagttt gcgtaccggc agcaatacgt ttaatacctg cgagatccgc atcctggccg
    12421 gaaattgcta ctttccctga taaaccttgc gcgcttaatg cctgaattgc cccacctgcg
    12481 gtggcatcgt ttgaggcaac tacagcatca attttgttat tattggcggt tagcgcgttt
    12541 tccataattt tcaatgcgtt ttccggtaac cagccatcaa cccattggtc accaacgact
    12601 ttaatttttc cggaatcaac gtaaggtttt aacactttca tttgtccggc gcggaacagc
    12661 ttggcgttgt tatctaccgg cgagccgccc atcaggaagt aattaccttg cggaacaata
    12721 tcgaccaggg cttttgcctg cagttcaccg actttttcgt tatcgaaaga aatataaaaa
    12781 tcgatatccg catcgttaat catacgatcg taagctaata ctttaatacc ttcttgtttg
    12841 gcttctttta caacgttact taatacctga ccgttatacg gaataatgac aagaacatca
    12901 acaccccggt ttatcatgtt ttcaatctgc gacatttgtg tttcttcatt gccatttgca
    12961 gactgtacaa atactttcgc gccgagagat tctgcctttt tcacaaaaat atctcgatct
    13021 ttttgccagc gttcaagacg gagatcatca atcgccatac ctattttgac ttctttggcg
    13081 tgtgccgcaa cgttggtaag cagaagtgag gtgcaaaggg tgagtagaat gttctttatt
    13141 ttcatggtgt agggccttct gtagttagag gacagcttta ataagtaaca atcaccgcga
    13201 taaacgtaac caatttttag caactaaaca ggggaaaaca attacagatt tttatctttc
    13261 gattacgatt tttggtttat ttcttgattt atgaccgaga tcttactttt gttgcgcaat
    13321 tgtacttatt gcatttttat cttcgaggaa ttacccagtt tcatcatccc attttatttt
    13381 gcgagcgagc gcacacttgt gaattatctc aatagcagtg tgaaataaca taattgagca
    13441 actgaaaggg agtgcccaat attacgacat catccatcac ccgcggcatt acctgattat
    13501 ggagttcaat atgcaagcct attttgacca gctcgatcgc gttcgttatg aaggctcaaa
    13561 atcctcaaac ccgttagcat tccgtcacta caatcccgac gaactggtgt tgggtaagcg
    13621 tatggaagag cacttgcgtt ttgccgcctg ctactggcac accttctgct ggaacggggc
    13681 ggacatgttt ggtgtggggg cgtttaatcg tccgtggcag cagcctggtg aggcactggc
    13741 gttggcgaag cgtaaagcag atgtcgcatt tgagtttttc cacaagttac atgtgccatt
    13801 ttattgcttc cacgatgtgg atgtttcccc tgagggcgcg tcgttaaaag agtacatcaa
    13861 taattttgcg caaatggttg atgtcctggc agccaagcaa gaagagagcg gcgtgaagct
    13921 gctgtgggga accgctaact gctttacaaa ccctcgctat ggcgcgggtg cggcgacgaa
    13981 cccagatcct gaagtcttca gctgggcggc aacgcaagtt gttacagcga tggaagcaac
    14041 ccataaattg ggcggtgaaa actatgtcct gtggggtggt cgtgaaggtt acgaaacgct
    14101 gttaaatacc gacttgcgtc aggagcgtga acaactgggc cgctttatgc agatggtggt
    14161 tgagcataaa cataaaatcg gcttccaggg cacgttgctt atcgaaccga aaccgcaaga
    14221 accgactaaa catcaatatg attacgatgc cgcgacggtc tatggcttcc tgaaacagtt
    14281 tggtctggaa aaagagatta aactgaacat tgaagctaac cacgcgacgc tggcaggtca
    14341 ctctttccat catgaaatag ccaccgccat tgcgcttggc ctgttcggtt ctgtcgacgc
    14401 caaccgtggc gatgcgcaac tgggctggga caccgaccag ttcccgaaca gtgtggaaga
    14461 gaatgcgctg gtgatgtatg aaattctcaa agcaggtggt ttcaccaccg gtggtctgaa
    14521 cttcgatgcc aaagtacgtc gtcaaagtac tgataaatat gatctgtttt acggtcatat
    14581 cggcgcgatg gatacgatgg cagtggcgct gaaaattgca gcgcgcatga ttgaagatgg
    14641 cgagctggat aaacgcatcg cgcagcgtta ttccggctgg aatagcgaat tgggccagca
    14701 aatcctgaaa ggccaaatgt cactggcaga tttagccaaa tatgctcagg aacataattt
    14761 gtctccggtg catcagagtg gtcgccagga gcaactggaa aatctggtaa atcattatct
    14821 gttcgacaaa taacggctaa ctgtgcagtc cgttggcccg gttatcggta gcgataccgg
    14881 gcattttttt aaggaacgat cgatatgtat atcgggatag atcttggcac ctcgggcgta
    14941 aaagttattt tgctcaacga gcagggtgag gtggttgctt cgcaaacgga aaagctgacc
    15001 gtttcgcgcc cgcatccact ctggtcggaa caagacccgg aacagtggtg gcaggcaact
    15061 gatcgcgcaa tgaaagctct gggcgatcag cattctctgc aggacgttaa agcattgggt
    15121 attgccggcc agatgcatgg agcaacctta ctggatgctc aacaacgggt attgcgccct
    15181 gccattttgt ggaacgacgg gcgctgtgcg caagagtgca ctttgctgga agcgagagtt
    15241 ccgcaatcac gagtgattac cggcaacctg atgatgcccg gatttactgc gcctaaattg
    15301 ctatgggttc agcggcatga gccggagata ttccgtcaaa tcgacaaagt attattaccg
    15361 aaagattact tgcgtctgcg tatgacgggg gagtttgcca gcgatatgtc tgacgcagct
    15421 ggcaccatgt ggctggatgt cgcaaagcgt gactggagtg acgtcatgct gcaggcttgc
    15481 gacttatctc gtgaccagat gcccgcatta tacgaaggca gcgaaattac tggtgctttg
    15541 ttacctgaag ttgcgaaagc gtggggtatg gcgacggtgc cagttgtcgc aggcggtggc
    15601 gacaatgcag ctggtgcagt tggtgtggga atggttgatg ctaatcaggc aatgttatcg
    15661 ctggggacgt cgggggtcta ttttgctgtc agcgaagggt tcttaagcaa gccagaaagc
    15721 gctgtacaca gcttttgcca tgcgctaccg cagcgttggc atttaatgtc tgtaatgctg
    15781 agtgcagcat cgtgtctgga ttgggtcgcg aaattaaccg gcctgtgcaa tgtcccagct
    15841 ttaatcgctg cagctcaaca ggctgatgaa agtgccgagc cagtttggtt tctgccttat
    15901 ctttccggcg agcgtacacc acacaataat ccccaggcga agggggtttt ctttggtttg
    15961 actcatcaac atggccccaa tgaactggcg cgagcagtgc tggaaggcgt gggttatgcg
    16021 ctggcagatg gcatggatgt cgtgcatgcc tgcggcatta aaccgcaaag tgttacgttg
    16081 attggtggcg gggcgcgtag tgagtactgg cgtcagatgc tggcggatat cagcggtcag
    16141 cagctcgatt accgtacggg gggggatgtg gggccagcac tgggcgcagc aaggctggcg
    16201 cagattgcgg cgaatccaga gaaatcgctc attgagttgt tgccgcagct accgttagaa
    16261 cagtcgcatc taccagatgc gcagcgttat gccgcttatc agccacgacg tgaaacgttc
    16321 cgtcgcctct atcagcaact tctgccatta atggcgtaaa cgttatcccc tgcctgaccg
    16381 ggtgggggat aattcacatc tatatatctc actaattaat taatatttag tatgaattta
    16441 ttctgaaaat catttgttaa tggcattttt cagttttgtc tttcgttggt tactcgtaat
    16501 gtatcgctgg tagatatgga gatcgttatg aaaacctcaa aaactgtggc aaaactatta
    16561 tttgttgtcg gggcgctggt ttatctggtt gggctatgga tctcatgccc attgctaagt
    16621 ggaaaaggct attttcttgg cgtgttaatg acagcaactt ttggtaacta tgcatatctt
    16681 cgcgcagaaa aactcgggca actggataat ttttttaccc atatctgcca gttagttgcg
    16741 ttaatcacta tcggtctctt gtttatcggt gttttaaatg cacctatcaa tgcttatgaa
    16801 atggtgatct accccatcgc cttttttgtc tgcttgtttg gtcaaatgcg tttgtttcgc
    16861 tcggtatgag caacataaag ctcttacaca ttcaggaatg aaaggaatac tgtgatggac
    16921 aacaaaatat caacctattc accggccttt agtattgtgt catggatagc tctcgttggt
    16981 ggtatcgtta cctatctgtt agggctatgg aatgcagaga tgcagttaaa tgaaaaaggg
    17041 tattattttg ccgtactggt gttaggactg ttttctgctg cgtcttatca aaagaccgtt
    17101 cgggacaagt atgaaggcat accgaccact tccatttatt atatgacctg cctgactgtc
    17161 tttattatct ctgttgcatt actgatggta ggtctgtgga atgcgacatt attactcagt
    17221 gaaaaaggtt tttatggact ggctttcttc ttaagcttgt ttggtgcagt agcggtgcag
    17281 aagaatattc gtgataccgg aataaacccg ccaaaagaaa cacaggttac ccaggaagaa
    17341 tacagcgaat aattcacgta agcccggtca gtccaatgtg aacgggcttt tacttaactc
    17401 actaatctgt ttctgtcgat tcgttgtacc agcatagaaa gtaacaaact cgctgccaac
    17461 gtcgcgcaaa agatccaaat aatatccagt attggccaat ttttaagctc aattccccgg
    17521 gtgcgcagcg catggataat caaggcgtgg aatccgtata tacccaatga atggcgggag
    17581 attaagccaa gtccgcgaat ggtacgcgta tccagcgtgt ttttaaccag agtcaatagc
    17641 gcgattgcgc agataaaaac catcggccca cagtaaagat accaggtatc ggcaaaattt
    17701 ccgcgccact gcaattcata taatgtcccg cgagagataa taaaaacccc cgtcgcaaac
    17761 agcgcggcgc tcacccacga cagtgcttta tgctgtgtgt ccatcatccc tatagcgcgg
    17821 cccaacatgc catacagaat gtagtaaaaa gtatcgccat tgatatataa gttaattggc
    17881 agccattcaa aaccgtcaat tttctgcggc actgtgtttg ggttagcgat aatgccaatc
    17941 accaccatta gcaccagcaa catttttccg ccgacgttct tcacctgaat cagcggtgaa
    18001 accagataaa tcaccgcaat cgcgaagaaa aaccacaagt ggtaaaacac tggcttttgc
    18061 agcaggtttt tcagcgctaa ctccatattg atggaggtaa acagcgcaat gtagagcagt
    18121 gcgattgcgc tataaaaaat cagacataag ccgatacgca agaaatggcg cggctgggcg
    18181 ctgcgttcgc caaaaaagag atagccggaa atcatgaaaa atagcggcac gctgacacga
    18241 gaggcagaat tcagaacatt ggcgatatcc catgtgacgg ggctaacact atgagcattg
    18301 gtcacatacc aggtagtggt gtgaatcatc accaccatta aacacgctat ccctcgcagg
    18361 ttatcaatcc agtaaatttt gggctgcatc tgtgtctctg tatctggtta aaaaaagtct
    18421 gaccgataaa tcatttggtt ggcgcactgg aataatctga gttttatcac tacagcttat
    18481 agaggcttaa ggaaattcgt aagatatcag ccactatacc gatataaata ataagactca
    18541 cctgcaaacc agacggtaat ttaatgatga tgaacgcttt ctttccggca atggcgctta
    18601 tggtgctagt gggttgttct acaccgtcac ccgtgcagaa agcacaacgg gtaaaggttg
    18661 atcctctgcg ttcgttgagt atggaagcgt tatgcaagga tcaggcggca aaacgttata
    18721 acaccggcga gcaaaaaatc gacgtcaccg ccttcgaaca gttccaggga agctatgaaa
    18781 tgcgcggtta taccttccgt aaagagcagt ttgtctgttc ttttgacgca gatggccatt
    18841 ttttgcatct ttccatgcgt taagccctgc tttttcccgt ttcgtactgt atatcttcca
    18901 tccagcgggt atactgatcc cttcctttaa atccacacgt atccagcacg aaataatatg
    18961 caaaagtttg ataccaggac cttccagggc ttgatcctga ccttacagga ttactgggct
    19021 cgccagggct gcaccattgt tcaaccattg gacatggaag tcggcgcggg aacctctcac
    19081 ccaatgacct gtctgcgcgc gctggggcca gaaccgatgg cggctgctta tgttcagcct
    19141 tctcgtcgcc cgaccgatgg tcgctacggc gaaaacccca accgtttaca gcactactat
    19201 cagttccagg tggtcattaa gccatcgccg gacaatattc aggagctgta cctcggttct
    19261 ctgaaagagc tgggcatgga cccgactatt cacgacatcc gtttcgtgga agataactgg
    19321 gaaaacccga ctctgggtgc ctggggactg ggctgggaag tgtggctaaa cggcatggaa
    19381 gtgacgcagt tcacttactt ccagcaggtt ggtggtctgg agtgtaaacc ggttaccggc
    19441 gagatcacct acggtctgga acgtctggcc atgtacattc agggcgtaga cagcgtttac
    19501 gacctggtct ggagcgacgg cccgctgggt aaaaccacct acggcgacgt gttccatcag
    19561 aacgaagtgg agcagtccac ttacaacttc gaatacgcgg atgtggactt cctgttcacc
    19621 tgctttgagc agtacgagaa agaagcgcag cagctgctgg cgctggaaaa tccgctgccg
    19681 ctgccagcct acgagcgtat tctgaaagcc gcccacagct tcaacctgct ggatgcgcgt
    19741 aaagccatct ccgtcaccga gcgtcagcgc tacattctgc gcattcgcac cctgaccaaa
    19801 gcagtggcag aagcatacta cgcttcccgt gaagccctcg gcttcccgat gtgcaacaaa
    19861 gataagtaag aggcggctat gtctgagaaa acttttctgg tggaaatcgg cactgaagag
    19921 ctgccaccaa aagcactgcg cagcctggct gagtcctttg ctgcgaattt tactgcggag
    19981 ctggataacg ctggcctcgc acacggcacc gttcaatggt ttgctgctcc gcgtcgtctg
    20041 gcgctgaaag tagctaacct ggcggaagcg caaccggatc gtgaaatcga aaaacgcggc
    20101 ccggcgattg cccaggcgtt cgacgctgaa ggcaaaccga gcaaagcggc agaaggttgg
    20161 gcgcgtggtt gcggtattac cgttgaccag gctgagcgtc tgactaccga taaaggcgaa
    20221 tggctgctgt atcgcgccca tgtgaagggc gaaagcaccg aagcactgct gccgaatatg
    20281 gttgcgactt ctctggcgaa actgccgatc ccgaaactga tgcgttgggg cgcaagcgac
    20341 gtgcacttcg tgcgtccggt gcacaccgtg accctgctgc tgggcgacaa agtcattccg
    20401 gcaaccattc tgggcattca gtccgatcgc gtgattcgcg gccaccgctt tatgggcgaa
    20461 ccggaattca ccatcgacaa cgccgatcag tatccggaaa ttctgcgtga gcgcgggaaa
    20521 gtcatcgccg attacgaaga acgtaaagcg aagattaaag ccgatgccga agaagcagcg
    20581 cgtaagattg gcggtaacgc tgacttaagc gaaagcctgc tggaagaagt ggcttcgctg
    20641 gtggagtggc cggtcgttct gaccgcaaaa ttcgaagaga aattcctcgc ggtgccggct
    20701 gaagccctgg tttacaccat gaaaggtgac cagaaatact tcccggtgta tgcgaacgat
    20761 ggcaaactgc tgccgaactt catcttcgtt gccaacatcg aatcgaaaga tccgcagcaa
    20821 attatctctg gtaacgagaa agtcgttcgt ccacgtctgg cggatgccga gttcttcttc
    20881 aataccgacc gtaaaaaacg tctggaagat aacctgccgc gcctgcaaac cgtgctgttc
    20941 cagcaacagc tgggtacgct gcgcgacaaa actgaccgca tccaggcgct ggctggctgg
    21001 attgctgaac agattggcgc tgacgttaac cacgcaaccc gtgcgggcct gctgtctaag
    21061 tgtgacctga tgaccaacat ggtcttcgag ttcaccgaca cccagggcgt tatggggatg
    21121 cactatgcgc gtcacgatgg cgaagcggaa gatgtcgcgg tggcgctgaa tgagcagtat
    21181 cagccgcgct ttgccggtga tgacctgccg tctaacccgg tagcctgtgc gctggcgatt
    21241 gctgacaaga tggatactct ggcgggtatc ttcggtatcg gccagcatcc gaaaggcgac
    21301 aaagacccgt ttgcgctgcg tcgtgccgca cttggcgtgc tgcgaattat cgttgagaag
    21361 aacctcaacc ttgatctgca aacgctgacc gaagaagcag tgcgtctgta tggcgataag
    21421 ctgactaacg ccaacgtggt tgatgatgtt atcgacttta tgctcggtcg cttccgcgcc
    21481 tggtatcagg acgaaggtta caccgttgac accatccagg cggtactggc gcgtcgtccg
    21541 actcgtccgg ctgatttcga tgcccgcatg aaagcggtat cgcacttccg tacccttgaa
    21601 gcagctgctg cactggcggc ggcgaacaag cgtgtctcca acattttggc gaaatctgac
    21661 gaagtgctga gcgaccgcgt gaatgcctct accctgaaag agccggaaga aattaaactg
    21721 gcgatgcagg ttgtggtgct acgtgacaag ctggagccgt actttgcgga aggtcgttac
    21781 caggatgcgc tggtcgaact ggcagagctg cgtgagccgg ttgatgcttt cttcgataaa
    21841 gtgatggtca tggttgatga caaagaattg cgtctcaacc gtctgaccat gctggagaaa
    21901 ctgcgcgaac tgttcctgcg cgttgcggat atttcgctgt tgcagtaata acgccgttat
    21961 taaaaagcct gccatctggc aggctttttt tattatcgct aaataataca gcaaccttta
    22021 ataatcttct gctgaataaa gattatctca tatattaatt ttatgaggtt ttttaggatt
    22081 atatcaagga gaagaaacaa acttattaag ctagaatagc cacgggtgct tgagactgtt
    22141 tgtctcaggt attcaccgaa aggcagacag agaaaagccc cacctgacta taaatcaaag
    22201 tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg aaatcaacac
    22261 agggggctgg gaatgccgca gaaatataga ttactttctt taatagtgat ttgtttcacg
    22321 cttttatttt tcacctggat gataagagat tcactgtgtg aattgcatat taaacagggg
    22381 agttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc ttcggcggga
    22441 agttattccc gcctttctta cggcgttgcg cattctcatt gcacccaaat ttatttttca
    22501 caaaaataat actagatttt attacgcgat cgattattta tttcctgaaa aacaaataga
    22561 aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga gattacaggc
    22621 tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag gacactttct
    22681 gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt acgaacacgt
    22741 ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg aaccatttta
    22801 cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt gtgcctttcg
    22861 gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta cgaggggtat
    22921 caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct gtacgtcaaa
    22981 ccgttgatgt gcattaagcc acgcattggc gggtgatgca acaattattt ttcatattta
    23041 tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat aaagtggata
    23101 aaagtctatt ccatcaactg cttacttaat gccgggttgg cttgaatcaa acgcatcaat
    23161 tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac catggcgact
    23221 gatacgccca aaacccgggc gaaatcatcg atttttaacc ctgtcccttt tcgtaactgc
    23281 tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta
    23341 aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata ttccattgaa
    23401 aactcctcaa atcacactgc ggggtcgtga attgcatcga agctcattaa gaatagtcgg
    23461 gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc gattcagcca
    23521 gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat atctcttacc
    23581 actcctggtt tgtaaagatt tcgaaaatct aaccttctgc gaaggcgtaa atctctggct
    23641 acaaaaaaag caacggcact atcatttttt aacaatgaaa caacaaatta accttgtttc
    23701 attttctcca tgattttacc aggaagatta cgtagaaagg gtatcctgca agcctgtcct
    23761 ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg
    23821 agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta
    23881 ttaatggtag ttagcctgag tgggtgtttt gataaagaag gcgatcagcg taaagcgttt
    23941 atcgacttcc tgcagaatac agtgatgcgt agcggtgaac gtctaccaac cctgactgcc
    24001 gatcagaaaa aacagtttgg tccttttgtc tctgattacg cgattctgta tggttattct
    24061 cagcaggtga atcaggcgat ggattccggt ctgcgcccgg tcgtagacag cgttaacgca
    24121 attcgcgtac cgcaggatta cgttacgcaa agcggtccgc tgcgtgaaat gaacggttcg
    24181 ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc aggccgatgc tgcgcactct
    24241 gcgctaaaac agaccgatga tttgaaaccg gtctttgatc aggcattcac caaagtggta
    24301 acgacgccag ctgatgcatt gcaaccgttg attccggctg cacaaacttt cacgcaacaa
    24361 ctggtgatgg ttggagacta cattgctcag caaggtactc aggtgagctt tgtcgcaaat
    24421 ggcatccagt tcccgacttc acagcaggca agtgaatata acaaactgat tgcgccatta
    24481 ccggcacaac atcaggcgtt taatcaggcc tggactacag cagttactgc aacccaataa
    24541 agagtaaaag cccgagcgat tatcgggctt tacttcacgc agtcgcggct tagtccgcga
    24601 cgtgcggatt cacacagttc ttctcaacct ttccttgtaa cgcatcaatc aaattatcca
    24661 cggcacaggc ggccatgcca taacgcgtct catgggtggc agatccaata tgcggtactg
    24721 cgacgacgtt ggccattgag agcaacggcg aatctacgga cagtggctct tgttcgaaga
    24781 catccagccc ggcggcgtga atttcgcctt tctgcaatgc agcaatcagt gcattttcat
    24841 caaccaccgg gccacgtccg gcattaatga aaatggcgga ggatttcatt ttggcgaatt
    24901 gttctgcgcc aaacagatga tgcgtctcat cagttaacgg caggatcagg caaacgaaat
    24961 ctgactcttg taacagagta tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt
    25021 ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt gaagccaaag tgcgcacgtt
    25081 gtgccagcgc cataccgatc cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt
    25141 cagtgccgta ccagtccggg cctatgctcg cggtccattc gcctgctttt acccgttctg
    25201 ctacctccac aacccgacga gcggtagaca acaccagcgc catcagcgta tcggcgacgg
    25261 tttctgttaa tacggttggc gtgtgcatca gcagaatttt tcgggcggta agcgcatcga
    25321 catcaaaatt gtcatagccg acggagatcg ttgatgtggc acgcagtttc ggcatttttt
    25381 ccagcaatgc ggcatcaaca ttctcgtttg aacccagtaa accttcagct tcggcaaaaa
    25441 ttgctgcatt ttgttcgacg gtttgtgggc tgaggtttgc cacctggtga acggtgaaat
    25501 gctcttgcag gcgttgcagt aaatcatcag gtaaggcttt gtagaggata acggacggct
    25561 tcatgcttct ctccattcac tgataaggcg atcaccttag cctgaaatat gatgaaggaa
    25621 aactgaagaa tgccaggtga tttctgcatc acctggcatg aaagggatta cagcgggctt
    25681 aaggtaattt ctacacggcg gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt
    25741 gggttagccg ggccaaggcc ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc
    25801 aacgcgctgg caacggaatc cgcacgttgc tgggagagac gcatgttcag gtcgtgacca
    25861 cccgtgctgt cggtataacc aatcacgtta accgccgttt tcggatactc tttcagcacc
    25921 attgccacgc cggtcagggt gttagcgccc gcaggtttca gggtcgcgct gctgctgtcg
    25981 aaggtcacat tgttcggcat attgaggata atgttatccc cgctgcgggt tacgctaaca
    26041 ccagtgccgc gcattttgtc acgcagcttc gcttcctgca catccatgta ataacccacg
    26101 ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc
    26161 ttcgaagaag agagcgcacc aatacccgcg cccacgagag agcccagacc tgcgccgata
    26221 gcagatttac ctgcttcgcg ttcgccggtg taagggttag ttgtgcagcc agataccgcc
    26281 agagcgccac tcactacggc ggcaataaga taaacacgtt tcttcattgt taatccttaa
    26341 taaccttttt attctttgcc acgggttccg tggcgggaga ttatgccgcg tgaacatgaa
    26401 gattattcct gggaatactc ggaaatttgt aagtaatatt taactgctca atacatctaa
    26461 cctttcagga gttttcggtt tggccaactc atcctcacga tattccgttc tgactgccgc
    26521 ccattggggg cccatgctgg ttgaaaccga cggcgaaacc gtgtttagct cgcatggcgc
    26581 gttagccaca ggaatggaaa actccttgca gagcgcggtt cgcgaccagg ttcacagcaa
    26641 tacacgggta cgatttccaa tggtgcgcaa aggctttctt gcgtcaccgg aaaatccgca
    26701 aggcattcgt gggcaggatg aatttgttcg cgtgagttgg gatgaggcgc tggatcttat
    26761 tcaccaacaa cataaacgca ttcgtgaggc ttatggtccg gcatcgattt ttgctggttc
    26821 ctacggctgg cgttcaaacg gcgtgctgca taaggcctcg acattattac aacgctatat
    26881 ggcgctggca ggcggttata ccgggcatct gggggattat tcgaccggcg cggcacaggc
    26941 gatcatgccg tatgtcgtgg gtggtagcga agtttatcaa cagcaaacca gttggccgct
    27001 ggtgctggaa catagcgatg tcgtggtgtt gtggagtgct aacccactca atacgctgaa
    27061 aattgcgtgg aatgcatctg atgagcaggg gctttcttac ttttccgcac tgcgtgacag
    27121 cgggaaaaag ctgatctgta ttgatccaat gcgatcggaa accgtcgatt tctttggcga
    27181 taaaatggag tgggtggcac cgcacatggg caccgatgtt gcgctgatgc tggggatcgc
    27241 ccatacgctg gtggaaaatg gttggcacga cgaagcgttt ctgacgcgtt gcaccacagg
    27301 ttatgccgtc ttcgcctctt atttgctggg cgagagtgac ggaatagcga aaaacgccga
    27361 atgggcggca gagatttgtg gggttaacgc agcgaaaatc cgcgagctgg cggctctttt
    27421 ccaccaaaac acgaccatgc tgatggctgg ctggggaatg cagcgccaac agtttggtga
    27481 gcaaaaacac tggatgatcg tcacgctggc ggcaatgttg gggcaaatcg gcacacccgg
    27541 cggcggtttt ggtctttctt atcattttgc caatggtggt aaccccacgc ggcgttctgc
    27601 ggtgctctct tccatgcagg gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc
    27661 tgttgcccgc attgttgaag cactggaaaa ccctggtggc gcatatcaac acaacggtat
    27721 gaaccgacat ttcccggata ttcgttttat ctggtgggcg ggcggtgcca actttactca
    27781 tcatcaggat accaatcgtc tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc
    27841 tgaatgcttc tggacggcgg cggcgaaaca cgcggatatc gttctgcctg cgactacctc
    27901 ttttgagcgt aatgatctca ccatgaccgg tgattacagt aatcagcatc tggtgccgat
    27961 gaagcaagtg gtgccgccac gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt
    28021 gagtgaacgt tgggagaagg gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca
    28081 atggctggaa acgttttata acgttgcccg gcagcgcggg gcaagccagc aggttgaatt
    28141 gccgccattt gctgagttct ggcaagccaa ccagttaatt gagatgccgg aaaacccgga
    28201 cagcgagcgg tttattcgct tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa
    28261 aaccgccagc ggcaagattg aaatcttctc acagcgtatt gccgattacg gttacccgga
    28321 ttgccctggg catccaatgt ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga
    28381 acagttgcag gtactttctg cccatccggc gcaccgcctg cacagccagc tgaattacag
    28441 ttctctgcgc gaattgtacg cggtagcaaa ccgtgagcct gtcaccattc atcctgacga
    28501 tgctcaggcg cgcggcataa ccgaagggga tatggtgcgg gtgtggaact cccgtgggca
    28561 gatccttgcc ggagcggtca ttagcgaggg aattaaacct ggcgtgattt gcattcacga
    28621 aggggcatgg ccggatctgg atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa
    28681 cgtgctgacc aaagatctcc ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc
    28741 gctggcatgg ctggaaaaat acaacggtcc ggaactgaca cttacagcgt ttgaaccacc
    28801 ggccagctca taatccaggt gggtagttgg gtttcatcct gccatgcgca atcgacaatg
    28861 tgaaaaccct gcgcacggta aaaatctatc gccggttgat ttttttgata aacctccagc
    28921 atcagatggg gatagcgctg ctgcacatac tgcatcagcg ccttaccaat accgcgtctg
    28981 acggccttcg gtgcgacaaa cattgctgcc agaaatcggc cttccataat gctgacaaaa
    29041 ccgagaagct taccgtcctc ttcccagacc cagttttgcg cgttggcaag ataggcatcc
    29101 cgcaccagcg gaatgcagtc acgccagtaa ttcgctttta taaagggatg cccccaggtt
    29161 gtactttcca gccacagttc gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc
    29221 atggtttatt tcccggatag caacagcagc caaccacatg atcattcacc agcccacatg
    29281 cctgcataaa ggagtaacag attgtggtgc cgacaaactt aaaaccacgt tttttcagtg
    29341 ccttagatag ggcgtcggag gcggacgtag atgtgggaat ttcgctcaac gttgtggctt
    29401 gtgtcacctg tggctgatga tttacaaacg accagacaaa gtcggcaaac ggttcgccgt
    29461 tctgttccat ttgcaggtac gcccgcgcat taccaataat tgcctgaatt ttccctcgat
    29521 ggcggataat cccggcgtcc tgtaccagtc tttcgacatc ctcttcctgc attgctgcga
    29581 ccttcaccgg atcgaactga tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg
    29641 tgatccacga taacccagcc tgctgccctt caaggcagat catttcgaac agttttttac
    29701 tgtcagtttc aggcacgccc cattcattat catggtaggc aatataaagc gggtcctgac
    29761 tcacccagcc gcaacgttcc atactttccc tcgctatgta cccgatcgta aaaatttcag
    29821 ccgcttttct tgacgcatcg gtgaaaaagt caatatttaa tacagggcta ctatgcccga
    29881 tatccttcat gcaatgacaa taatattgcc gaattcggct cttccctgga gtcgtgttaa
    29941 tgatcataaa aaaaagcggt ggtcgctggc agctaagcct gctggcgagc gtggtaatca
    30001 gtgccttttt tctcaacaca gcttacgcct ggcaacaaga atatatcgtt gatacgcaac
    30061 ccggacattc cacagagcgt tacacctggg atagtgatca tcaacctgat tacaacgata
    30121 ttttgtcgca acgtattcaa agtagccaaa gggcgctggg actggaagtc aatctggcgg
    30181 aagaaactcc tgtggatgtg accagcagta tgagtatggg ctggaatttt cctttatatg
    30241 aacaggttac aaccggcccg gtcgcggcat tacattacga tggcacaacc acctcgatgt
    30301 ataacgagtt tggcgacagt actaccacgc tgaccgatcc gttatggcat gccagcgtga
    30361 gtagcttagg ctggcgtgtt gactcccggc ttggcgatct ccgaccctgg gcgcaaatca
    30421 gctataacca gcaatttggc gagaatatct ggaaggcgca atcaggcctg agccggatga
    30481 cggcgacaaa ccagaacggc aactggctgg atgtcaccgt aggcgctgat atgttgctca
    30541 atcaaaatat tgccgcctat gccgcgctaa ctcaggcaga aaataccact aataatagcg
    30601 actatctgta tacgatgggg gttagcgcca gattttaacg taacagtcac aattgaaacc
    30661 attgaataac aacagttgtg gcgatagtgg gtgctaactt accaaataat aaatttggtg
    30721 aataattgtc gcgtcattca ttcctgaact aaggcatttc attccgttct gatggcattt
    30781 catgccgttt ttccccaggc ataaaatgca cttcgttatg gttgtcggca gagatttttc
    30841 ctttttatta ctgcaggaat actgccatga caccttcaaa ttatcagcgt acccgctggc
    30901 tgacactcat cggtactatc attacccagt ttgcactggg gtcggtttat acctggagcc
    30961 tgtttaatgg cgcgctttct gccaagctgg atgcgccggt aagccaggtc gctttctctt
    31021 tcggcttgtt aagtctgggg ctggcaattt cgtcttctgt tgcgggcaaa ttgcaggaac
    31081 gttttggcgt taaacgcgtc accatggctt ccggcatttt gctgggatta ggcttcttcc
    31141 tgaccgcgca ttccaacaac ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc
    31201 tggcggatgg cgcgggttac ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc
    31261 gtaaaggtct gatatccgcg ttcgctatcg gttcttatgg tctgggtagc ctgggtttca
    31321 aatttatcga cacgcagctg ctggaaacgg tgggtctgga aaaaaccttt gtgatttggg
    31381 gagcgattgc gctggtgatg attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac
    31441 aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta cacgctggca gagtcgatgc
    31501 gtaaaccgca gtactggatg ttagcggtaa tgttcctgac cgcctgcatg agcggcctgt
    31561 acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc acaccttgat gtggtttccg
    31621 cagccaatgc agtcactgtt atttccatcg ccaacctttc aggtcgtctg gtgctgggta
    31681 ttctgtctga caaaatcgcc cgtatccgtg ttatcaccat tggtcaggtg atatcgctgg
    31741 tgggtatggc ggccctgctg tttgcaccat tgaatgcagt gacgttcttt gcagcgattg
    31801 cctgcgtggc atttaacttt ggcggcacta ttaccgtctt tccgtcactg gtcagtgagt
    31861 tctttggcct caataacctg gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg
    31921 gtagcatttg tggttcgatt atcgcctcac tgtttggcgg cttctatgtg accttctacg
    31981 tgattttcgc cctgctgatt ctgtcattgg cgctttctac gacaattcgt cagccagagc
    32041 agaaagtatt gcgtgaggcg catatttaga aatatgttgc tactgaaata aatgcgagga
    32101 gggagacttc aaagtctccc tt
//
LOCUS       DAZFRJ010000034        32021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000034 DAZFRJ010000000
VERSION     DAZFRJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.034"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..200
                     /locus_tag="ACHKUN_001442"
     CDS             <1..200
                     /locus_tag="ACHKUN_001442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659734.1"
                     /translation="PCLNESDEHKGNQYSRWISVVLGELLIKIWNCDTVQIQTHKKTS
                     SLALERFLADLITINYEAQKK"
     gene            235..2940
                     /gene="malT"
                     /locus_tag="ACHKUN_001443"
     CDS             235..2940
                     /gene="malT"
                     /locus_tag="ACHKUN_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO6659735.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2983..3999)
                     /gene="rtcA"
                     /locus_tag="ACHKUN_001444"
     CDS             complement(2983..3999)
                     /gene="rtcA"
                     /locus_tag="ACHKUN_001444"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO6659736.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4003..5229)
                     /gene="rtcB"
                     /locus_tag="ACHKUN_001445"
     CDS             complement(4003..5229)
                     /gene="rtcB"
                     /locus_tag="ACHKUN_001445"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO6659737.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5418..7016
                     /gene="rtcR"
                     /locus_tag="ACHKUN_001446"
     CDS             5418..7016
                     /gene="rtcR"
                     /locus_tag="ACHKUN_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO6659738.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6998..7756)
                     /locus_tag="ACHKUN_001447"
     CDS             complement(6998..7756)
                     /locus_tag="ACHKUN_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO6659739.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7773..8603)
                     /gene="glpG"
                     /locus_tag="ACHKUN_001448"
     CDS             complement(7773..8603)
                     /gene="glpG"
                     /locus_tag="ACHKUN_001448"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO6659740.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8648..8974)
                     /gene="glpE"
                     /locus_tag="ACHKUN_001449"
     CDS             complement(8648..8974)
                     /gene="glpE"
                     /locus_tag="ACHKUN_001449"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO6659741.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9164..10669
                     /gene="glpD"
                     /locus_tag="ACHKUN_001450"
     CDS             9164..10669
                     /gene="glpD"
                     /locus_tag="ACHKUN_001450"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO6659742.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10721..11326)
                     /locus_tag="ACHKUN_001451"
     CDS             complement(10721..11326)
                     /locus_tag="ACHKUN_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659743.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(11329..12096)
                     /locus_tag="ACHKUN_001452"
     CDS             complement(11329..12096)
                     /locus_tag="ACHKUN_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659744.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(12099..12809)
                     /locus_tag="ACHKUN_001453"
     CDS             complement(12099..12809)
                     /locus_tag="ACHKUN_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659745.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCTRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(12825..14333)
                     /locus_tag="ACHKUN_001454"
     CDS             complement(12825..14333)
                     /locus_tag="ACHKUN_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6659746.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(14459..16906)
                     /gene="glgP"
                     /locus_tag="ACHKUN_001455"
     CDS             complement(14459..16906)
                     /gene="glgP"
                     /locus_tag="ACHKUN_001455"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO6659747.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(16925..18358)
                     /gene="glgA"
                     /locus_tag="ACHKUN_001456"
     CDS             complement(16925..18358)
                     /gene="glgA"
                     /locus_tag="ACHKUN_001456"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO6659748.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(18358..19653)
                     /gene="glgC"
                     /locus_tag="ACHKUN_001457"
     CDS             complement(18358..19653)
                     /gene="glgC"
                     /locus_tag="ACHKUN_001457"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO6659749.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(19671..21644)
                     /gene="glgX"
                     /locus_tag="ACHKUN_001458"
     CDS             complement(19671..21644)
                     /gene="glgX"
                     /locus_tag="ACHKUN_001458"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO6659750.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(21641..23827)
                     /gene="glgB"
                     /locus_tag="ACHKUN_001459"
     CDS             complement(21641..23827)
                     /gene="glgB"
                     /locus_tag="ACHKUN_001459"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO6659751.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(24100..25203)
                     /gene="asd"
                     /locus_tag="ACHKUN_001460"
     CDS             complement(24100..25203)
                     /gene="asd"
                     /locus_tag="ACHKUN_001460"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6659752.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            25395..25988
                     /gene="yhgN"
                     /locus_tag="ACHKUN_001461"
     CDS             25395..25988
                     /gene="yhgN"
                     /locus_tag="ACHKUN_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO6659753.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(26045..27385)
                     /gene="gntU"
                     /locus_tag="ACHKUN_001462"
     CDS             complement(26045..27385)
                     /gene="gntU"
                     /locus_tag="ACHKUN_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6659754.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(27389..27916)
                     /gene="gntK"
                     /locus_tag="ACHKUN_001463"
     CDS             complement(27389..27916)
                     /gene="gntK"
                     /locus_tag="ACHKUN_001463"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6659755.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(28055..29050)
                     /gene="gntR"
                     /locus_tag="ACHKUN_001464"
     CDS             complement(28055..29050)
                     /gene="gntR"
                     /locus_tag="ACHKUN_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO6659756.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(29274..29969)
                     /gene="yhhW"
                     /locus_tag="ACHKUN_001465"
     CDS             complement(29274..29969)
                     /gene="yhhW"
                     /locus_tag="ACHKUN_001465"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO6659757.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(30092..31129)
                     /gene="yhhX"
                     /locus_tag="ACHKUN_001466"
     CDS             complement(30092..31129)
                     /gene="yhhX"
                     /locus_tag="ACHKUN_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6659758.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            31462..31950
                     /gene="yhhY"
                     /locus_tag="ACHKUN_001467"
     CDS             31462..31950
                     /gene="yhhY"
                     /locus_tag="ACHKUN_001467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6659759.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         7914 a         8643 c         7992 g         7472 t
ORIGIN      
        1 acccttgctt aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg
       61 tcgttttagg tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac
      121 ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt
      181 acgaagcgca aaaaaaataa tatttcctca ttttccacag tgaagtgatt aactatgctg
      241 attccgtcaa aactaagtcg tccggttcga ctcgaccata ccgtggttcg tgagcgcctg
      301 ctggctaaac tttccggcgc gaacaacttc cggctggcgc tgatcacgag tcctgcgggc
      361 tacggaaaga ccaccctcat ttcccagtgg gcggcaggca aaaacgatat cggctggtac
      421 tcgctggatg aaggtgataa ccagcaagag cgtttcgcca gctatctcat tgccgccgtg
      481 cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat
      541 gccagcctga cgtcactctt cgcccagctt ttcattgagc tggcggaatg gcatagccca
      601 ctttatctgg tcatcgatga ctatcatctg atcactaatc cagtgatcca cgagtcaatg
      661 cgcttcttta ttcgccatca accagaaaat ctcaccctgg tggtgttgtc acgcaacctt
      721 ccgcaactgg gcattgccaa tctgcgtgtt cgtgatcaac tgctggaaat tggcagtcag
      781 caactggcat ttacccatca ggaagcgaag cagttttttg attgccgtct gtcatcgccg
      841 attgaagctg cagaaagcag tcggatttgc gatgacgttt ccggttgggc gacggcacta
      901 cagctaatcg ccctctccgc ccggcagaat acccactcag cccataagtc ggcacgccgc
      961 ctggcgggaa tcaatgccag ccatctttcg gattatctgg tcgatgaggt tttggataac
     1021 gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat
     1081 gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa tgcgtctcga agagattgag
     1141 cgtcaggggc tgtttttaca gcggatggat gataccggcg agtggttctg ctatcacccg
     1201 ctgtttggca acttcctgcg ccagcgctgc cagtgggaac tggcggcgga gctgccggaa
     1261 atccaccgtg ccgccgcaga aagctggatg gcccagggat ttcccagcga agcgattcat
     1321 catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg
     1381 agtttgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg
     1441 gacagtttgc tggaaaatcc gcagttagtg ttattgcagg cgtggctaat gcaaagccaa
     1501 catcgctatg gcgaagttaa caccctgcta gcccgtgctg aacatgaaat caaggacatc
     1561 agagaaggca ccatgcacgc agaatttaac gctctgcgcg cccaggtggc gattaacgat
     1621 ggtaatccgg atgaagcgga acggctggca aaactggcac tggaagagct gccgccgggc
     1681 tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc
     1741 gaattgaccc gctcactggc gctaatgcag caaaccgaac agatggcacg ccagcacgac
     1801 gtctggcact acgccttatg gagtttaatc cagcaaagtg aaattctgtt tgcccaaggg
     1861 ttcctgcaaa ccgcgtggga aacacaggaa aaagcgttcc agctgatcaa cgagcagcat
     1921 ctggaacagc tgccgatgca tgagtttctg gtgcgcatcc gagcacaact gttatgggcc
     1981 tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct
     2041 tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt
     2101 ggtgatttag ataacgcccg tagccagctg aaccgtctgg aaaacctgct ggggaatggc
     2161 aaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg
     2221 accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg
     2281 aacaaccact tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat cttgctgggc
     2341 gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg
     2401 ttgatgagcg atctcaaccg taacctgttg ctgcttaatc aactgtactg gcaggccgga
     2461 cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga
     2521 tttatcagcc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt
     2581 caactcaata cgctgccgga actggaacag catcgcgcac agcgtattct gcgagaaatc
     2641 aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga acgtctgcta
     2701 aatcatccgg aagtgccaga actgatccgc accagcccgt tgacccaacg tgaatggcag
     2761 gtcctggggc tgatttactc cggttacagc aatgaacaaa ttgcgggcga actggaagtg
     2821 gcggcaacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac
     2881 cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga
     2941 gtttagccgg ataatgcatt ggatccggtt tacatcttta catcattcaa tactcacccg
     3001 cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc
     3061 gatattggtc agcagatggc atgaaggatg ggcgacctta aattctcccg cgcccgccag
     3121 cgccatcggc agtaccagtt ggtcggcgag atattccccc accgccgctg ggcttgccag
     3181 gtagcgtttc acctctttca ctaactgcgc ggctaccacc tcggcactga cgcgcttttc
     3241 accgacgacg aaaaagcgtt cagtgatatt ttcactttcg acttccagcg agacggtatt
     3301 acccggcccc tggtcgcgcg gcaggttatg aatattctgt tcatgcaggg aaaaactccc
     3361 cgccagtgta gcgatttcac gctcagcaac atggcgcggc acaccagcta atagaacttc
     3421 tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa acgatgccac
     3481 gggcgagact tccgttgcca ccacgccgcc tccggcagga taaaaaccgt gacgtaacag
     3541 cgtggtttgc tgatgaattc ctattttcgc cagcagcggc tccagcaccc ggcggataaa
     3601 atccgcaggc ggggccgacg ggttatcggt gcctccgctc acttcaacac gcgaaggtcc
     3661 atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc agcgtacagc ttccggcact
     3721 accgatagca aagcggtact cgccgccgcg cacggtgcca ggccggaaga ccagacgctg
     3781 cgaccccagc tccgcgcctt ccaccgttgc cctacaaatt tccgcagccg cttttaccgc
     3841 ggtcagatgc tggcgcaaca gccccggttt cgcccgcccg gcacgaatgc tggtgatggt
     3901 aaatggctgg ccggttatca tcgacaggct cagcgccgag cgcaggatct gcccgccacc
     3961 ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt ttacgcacac
     4021 cacctgacgc agggtataga taacttccac cagatcgctt tgtgccgcca tcaccgcatc
     4081 aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac ggcattccac
     4141 atgcgcggtg gcacgaattt gatcttccac gctgaacagt tttttcgctt tagtacggct
     4201 cattacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc ccagcccacg
     4261 gacgataaag ctttttgctc ccatcgaacc gggaataatt ccatattgac cagcacgcgc
     4321 agacaccgcg cctttacgcg tcacgtagat ctcttcacca aagtgctgtt ctttttgcac
     4381 atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc tgaccgtttt
     4441 ctgcgtaatg ctctgcaacg ccgttaccac gttttccatc atcgcatcgc ggttaaggct
     4501 ggcgaaaagc tgcgcccagg ccacggctct taggtaatca tcaaagtatt ccgtaccttc
     4561 cataaagtac gccaggtcgc gcgatggcaa cgtttcaagc tggtcctgca tctctttttg
     4621 tgccaaatcg ataaagtaag tcccgatggc gttaccaatt ccgcgtgaac cggagtgcag
     4681 cataatccac acctggtccg actcatcaag gcagatttca ataaagtggt tacccgttcc
     4741 cagcgttccc aggtgtttat agttgttagt gttaaggaaa cgcggatatt tttgcgttaa
     4801 ccactgataa ccggcttcaa gctcagccca tttcgcatcg acgttaacag gcgggttttc
     4861 ccatgcgcct ttatcacgtt tacaacggcc agtggtacgt ccgtgcggca ccgcagtttc
     4921 aatcgcctga cgcagctctg ccaggttttc aggcaggtct gccgccgtta acgcggtacg
     4981 cagcgcgttc attccacagc caatatccac gcccaccgcc gccggaataa tcgccccttt
     5041 ggtcgggatc acgctaccaa tggtggaacc tttacccagg tgtacatcag gcattaccgc
     5101 aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac gcgcatcggc
     5161 ttctaccggc acgcctttgg tccacatttt taccggggca ttttcagtgg tcagtaattc
     5221 gtaattcatt ttgtttttct cttttcgttg tttgctgtcg tgataattgc aaccgtcgtg
     5281 ccagaaaatt aaataagcag attaattttt taattcattg tttttaaata ggttaaatct
     5341 ttacgtccgt aaacggagat tttccacaaa accaattcac cgataatgaa atatcgtctt
     5401 ttataaggat atctaagatg cgtaaaacag tggcttttgg ctttgtcggt accgtgctgg
     5461 attatgccgg acgcggcagt cagcgctggt cgaaatggcg tccgacgctt tgtttatgcc
     5521 agcaagaatc gttagtcatc gatcggctgg aattgttgca cgacacccgc tcgcgctcgc
     5581 tattcgaaac gcttaaacgc gatatcgcca gcgtttcgcc agaaacacaa gtcgtgggcg
     5641 ttgagattga actgcataac ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt
     5701 tcgcccgtgg ttacgagttt cagccagaaa aagaagacta tttaattcac atcaccaccg
     5761 gcacccacgt cgcgcagatt tgctggtttc tgctggcgga agcgcgttac ctgcccgccc
     5821 ggctgataca atcttcacct ccgcgcaaaa aagaacagcc gcgcggcgca ggtgaagtga
     5881 cgattatcga tctcgattta agccgttata acgccatcgc cagccgcttt gccgaggaac
     5941 gccagcaaac gcttgatttt cttaagtccg gcattgccac gcgtaacccc cacttcaacc
     6001 gcatgattga gcagatcgaa aaagtggcga tcaaatcccg cgcgccgatt ctgcttaacg
     6061 gtccaaccgg cgcgggcaag tcatttctgg cgcgacgcat cttcgagtta aaacaggcgc
     6121 ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg
     6181 ccatgtcgac gctgtttggt catgtaaaag gcgcgtttac cggggcgcgg gaatctcgtg
     6241 aaggtttatt acgcagcgcc aacggcggaa tgttgtttct tgatgagatt ggcgaactgg
     6301 gcgcagacga acaggcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg
     6361 gcagcgatcg ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc
     6421 gccagctggt tgccgaaggc aaatttcgcg aagatctgta cacgcggatc aatctctgga
     6481 ccttcaccct gccgggtcta cgccagcgcc aggaagatat tgaaccgaac ctggattatg
     6541 aagtggagcg ccacgcctca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc
     6601 gcgcctggtt ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac
     6661 tttctgccag cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg
     6721 tggttgaaga tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc
     6781 ttacggcgtt gctgggcgca gaggcagaaa acatcgatct cttcgaccgt atgcaactgg
     6841 aacacgttat cgctatctgc cgccaggcaa agtcgctttc cgcagccgga cgccagcttt
     6901 ttgacgtttc gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc
     6961 tggcgcgttt tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat
     7021 ggtggtccgt cagcacctgc atcacgctta ctggcggcgg ggcgtcggtg tagacggcat
     7081 ctaccatgct gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga
     7141 caaccagcat aacgtggcgc gagttctcaa taatggcgcg tttggtgcga acttcgtggt
     7201 aatcgaactc cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat
     7261 caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc
     7321 tgcgtaattc gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag
     7381 caacattgag attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca
     7441 ccgcttccgg cgtggtgccg atatcgataa acagtgtcga gccattgggg atttgctccg
     7501 ccactttgcg ggcaatcctc tctttttctt cggtctgggt ggctttacga tcgtgccacg
     7561 gcgtgttaac cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt
     7621 gctccgccag ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta
     7681 ccagctcttc agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac
     7741 gttgtgtttg tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa
     7801 tcaacaaaag ccatcgctaa acccacggct aacccggcga tgtgtgctcc gttcgccatc
     7861 gacatcccaa acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac
     7921 ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc cacgtagcca gacgtagccc
     7981 atcagcgcat acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc
     8041 acatagccgc ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg
     8101 cgtttttcca ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc
     8161 agcgagaagt gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgttgga
     8221 tcgaatggcc aggccagcca taacatcact tcctgatcgc cgagaatttg catggcgata
     8281 aacaccacca cgcaggcgat catcaccacc caggttaccg gacctgcgcg ttcacgcaag
     8341 gcagcaaaga aaggataacg gcgataatgc agaccactgc cggtatggcc cgcctgccag
     8401 ctggccgcca gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgc
     8461 acgcgctcgg cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc
     8521 gtgaggataa caccctgcgt cgccatataa tcaacaaacg cctgcgccac gcgggggtta
     8581 gcaaaagagg taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta
     8641 taaagcgtta cgcgccgtac gccacctctg cgggaaactg acgctgccag gcttcaaaac
     8701 cgccgtcaat gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt
     8761 tgctgctatt gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca
     8821 taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgc cccatagcga
     8881 aactctgtgg atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg
     8941 cgtcggcaac gttaatacat tcgaacagat ccatgcgtct ctctttcttt acaaacaagt
     9001 gggcaaattt accgcacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat
     9061 atcactctaa aatgtttttt caatgttacc taaagcgcga ttctttgcta atatgttcga
     9121 taacgaacat ttataagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct
     9181 gattgtgata ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg
     9241 tttatccgtg ctgatgctgg aggcgcagga tctcgcttgt gcgacctctt ccgccagttc
     9301 aaaactcatt cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga
     9361 ggcgctggct gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccgatgcg
     9421 ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg atgattcgga ttggtctgtt
     9481 tatgtacgat catctgggta aacgcaccag cctgccggga tcaaccggtt tgcgttttgg
     9541 cgcaaactca gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt
     9601 agacgacgcc cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt
     9661 gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc
     9721 ggaagatatc gataccggca aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac
     9781 cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc cttatggcat
     9841 tcgcctgatc aaaggcagcc atattgtggt gccgcgtgtg catacccaga agcaggccta
     9901 cattctgcaa aacgaagata aacgtattgt gttcgtgatc ccgtggatgg acgaattttc
     9961 catcatcggc accaccgacg tagagtataa aggcgatccg aaagcggtga agattgaaga
    10021 gagtgaaatc aactatctgc tgaaagtgta taacgcgcac tttaaaaaac agcttagccg
    10081 tgacgatatc gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc
    10141 gccgcaggct attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc
    10201 gctgctgtcg gtattcggcg gtaagctgac cacctaccga aaactggcag aacatgcgct
    10261 ggaaaaactg acgccgtatt atcagggtat tggcccggcg tggacgaaag agagcgtgct
    10321 gccgggtggt gccattgaag gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta
    10381 tccattcctg actgaatcgc tggcgcgtca ttacgcacgc acttacggca gcaacagcga
    10441 gctgctgctc ggcaatgcgg gggcgataag cgacctcggg gaggatttcg gtcatgagtt
    10501 ctacgaagcg gaactgaaat acctggtgga tcacgaatgg gtccgccgca ccgacgatgc
    10561 cctttggcgt cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag
    10621 tcagtggctg gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc
    10681 ggttgaagtg caaccgggct atttggattc cttagactat tcattgtaaa taattgaata
    10741 acggcccctg agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc
    10801 tctggtttct attttttctg caccaatggc aataatatcc ccatgcaaaa ccaagctgtg
    10861 cactaatcgg gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg
    10921 gaggccagac aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg
    10981 gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca
    11041 gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga
    11101 tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtaattcct
    11161 gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta
    11221 acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa
    11281 caaaaccgag aattacaggg ggaatataaa gccggaattt aatcattttt attttcctgg
    11341 tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcatcttc
    11401 ccagacctga tattctgtca ttggttcggg ctgttttagc gggttctcaa tcgcttgccg
    11461 cgctctttct ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat
    11521 ggcgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa
    11581 acaggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg
    11641 cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc
    11701 atcgccggat acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc
    11761 atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg
    11821 aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac
    11881 ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata
    11941 cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata
    12001 ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg
    12061 ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg
    12121 cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta
    12181 cgcgaaaggg aaaagctggg gcgagtgcaa ctattgcagt ctcgacagct ttcctctgcg
    12241 caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat
    12301 ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg aaagatatag
    12361 acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca
    12421 tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat
    12481 tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa
    12541 agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct ggaggttaag
    12601 ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc accgacgaac
    12661 ttttcccctt catccagggc ctggttaata agttgctgaa caatgccttc atcccagtat
    12721 gccgatttgt agttggcaat cgtaatatcc acgcctgtga agggatcagt accggcaagg
    12781 cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg tagcgtctta
    12841 tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt taaaaaccac
    12901 tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttatcataaa ggcatcgacg
    12961 atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct
    13021 agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct
    13081 attgcgttgt ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt
    13141 gccacttctg tttcctgcgg cgagatcatg cggacatttt tcatgacctg ggattgcatc
    13201 accgtctggc taccagagtc ttccgggcgt tgccaggtct ggatctcctg atcgttaccg
    13261 ccaacagtac gccagttggt aattgcacca ctgaagatgt cacgcacctg ttgttcagtc
    13321 agggaattaa ccggattatc cgcattgacg atgaaaacaa acgcttcacg ggcaaaaggc
    13381 gtgtacagca aagtaacgcc cgattcctca gcgcgttttt tctgcccacc ggaaggttgc
    13441 gccacaaaaa taatatcagc atcaccttta acaatcctgt tatacgcatc gggggtacga
    13501 gagctctcca gatattccct ggtgtgaaaa tcttccggta taacgcttaa agcataaaat
    13561 gcagaagcat aaattgggta cgccgccgta gcaccatcga gtcgcggcca gttttgcgta
    13621 aactgaattt gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc
    13681 caggcccagg tatcaatatt ttcgcgcaca ccgacgccgg gattgagctg tttatataaa
    13741 taaccctgat aaccagcgac aaaaccaagc atgagaagga taaacaggag ggaattacgg
    13801 caacgtagcc cggatgtacc ggttaaagga tgtctggttg gccggccgta ccccagcgaa
    13861 aaactgatat gcgcgccaac gggtataatg actatccacc agtactcacc tccgactact
    13921 gagactaaca aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgatcccatc
    13981 tggtgaaatt gataatctgc cagatgcagc gcgattatcc agccaaccag gattaataaa
    14041 ccgggtacta aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta
    14101 tcgcctgccc atactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa
    14161 atgttgagca gaaggtaact tgttggcgtg ataaggatat tacaaacttc aataataccg
    14221 gcaagtccga cacccagcat agcaacaacc gccgccaaaa attgcgtcag tatggggatg
    14281 ccgaaaaaag tcattaccag cgaggacaaa atccattttc tgttttgcat tattctttcc
    14341 attctttttg aatggtgaaa ttatactccc cgagtcccct tgccccttct ggacactttt
    14401 ccgaaatgat ggcggaaaaa aacgggagcc tttggccccg ttctatttat tggtgaactt
    14461 acaatctcac cggatcgata tgccagatat gatcggcgta ctctttgata gtacggtcag
    14521 aagagaagta gcccatattg gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa
    14581 gctcgtagag ttcgtcgact ttatcctgac aatcgacata gctgcgataa tccgccagaa
    14641 cctgatagtg atcgccgaag ttgatcagcg aatcaaccag atcgcgatag cgacccggat
    14701 cttccggact gaatacaccg ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt
    14761 tctcgtagta ttcacgcggt ttgtagccct gacgacgcag ttcttccact tcttccgcag
    14821 tgttaccaaa gataaagata ttgtcagcac cgacatgatc cagcatctcg acgttcgcac
    14881 cgtccagcgt accgatagtc agcgcgccgt taagcgcaaa cttcatgtta ctggtgccgg
    14941 aagcttccgt ccctgccagc gaaatctgtt cagacagatc tgccgccgga atgatcaact
    15001 gcgccaggct aacgctgtag ttcgggatga acacgacttt cagcttatcg ccaatctgcg
    15061 gatcgttgtt gatcactttc gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt
    15121 aataggccga agccgcctta ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg
    15181 ggtcggcctt gatgcggtta tagcgggtaa tcacatgcaa cacattcatc aattgacgtt
    15241 tgtattcgtg aatacgtttg atttgtacat cgaacaacgc ctttggattc accaccacat
    15301 tcagctgctg ggcgatatac tctgccagac gctttttgtt ctccagcttc gcctgatgca
    15361 cagcgtgatt aaccattggg aaatcacagt gttgttgcag ctcattaagc aggctaaggt
    15421 cggtgcgcca gttacggccc aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta
    15481 ccgctagcca gcgacgcggc gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga
    15541 ttttcgcaaa gtcggcaaac aacgattgca ccatcagatt agagtgcagt tccgatacac
    15601 cgttaacttt gtggctcaca acaaccgcca gccaggccat acgcacacga cgaccgttgg
    15661 attcatcaat gatcgacgcc cgtcccagca gatcggtatc gttcggatac tgttcctgca
    15721 aggttttcag gaaatagtcg ttgatttcaa agatgatctg caggtgacgc ggcagaattt
    15781 tacccagcat atcaaccggc caggtttcca gcgcctcgct catcagcgta tggttagtgt
    15841 aggagaagac ctgacaacac acctcaaacg cgtcgtccca gctaaattgg tgctcatcga
    15901 tcagcagacg catcatctca ggaatcgaca gtaccggatg ggtatcattg agatgaatcg
    15961 cgattttatc cgccaggtta tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt
    16021 cctgaatggt cgaggaaacc aggaagtatt cctgacgcag gcgcagctca cgcccggagt
    16081 aggtggagtc atccggatac agtacgcgag atacgttctc ggagtggttt ttatcttcca
    16141 ctgccgcgaa gtagtcaccc tggttgaatt taccgaggtt aatttcgcta ctggcttgcg
    16201 cactccacaa acgcaacgtg ttggtcgcgt cggtgtcgta accagggatt atctgatcgt
    16261 aagcgactcc cagaatctct tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa
    16321 tgcgaccacc aaaacggact ttatagcgcg tgttgtggcg tttgaattcc cacgggttac
    16381 cgtattccag ccagtagtct ggcgactctt tctggctacc gttaacgatg ttctgcttga
    16441 acataccgta gtcatagcgg atgccgtaac cgcgccccgg caaccctaac gtcgccagag
    16501 aatcaaggaa gcaagccgcc agacgtccca ggccaccgtt accgaggcct gggtcatttt
    16561 cttcatcaat cagctcttcg agatttaacc ccatcgcttc cagtgcgccc tgtacatctt
    16621 cgtaaattcc tagcgacaac atggcgttgg agagcgtacg gccaatcaaa aactccatcg
    16681 acaggtagta aacctgacga gtttcttgcg acaactgggc acggtttgaa cgtaaccagc
    16741 gctccacgag acgatcgcgc acagcaaata acgttgcgtt cagccattca tgtttattgg
    16801 cgacgaccgg gtcctttcca atcgtaaaca tcagcttgta agcgatagag tgcttaagag
    16861 cttctacgct aagcgtgggc gatgaatatg taaacggagc attcatatag gcgtttcctg
    16921 aaaactattt caagcgatag taaagctcac ggtacgactt cgccgcgacc tgccagctaa
    16981 aatccattgc catagcctga cgttgcacaa accgccacag tgaaggacgg gaccacagta
    17041 caaaagcacg tcgaatagcc cgtaacagcg accaggcatt actatcttca aagacaaacc
    17101 cactggcgac gccatctgca aggttctcaa gagaacagtc agaaaccgta tcagcaagcc
    17161 caccggtgcg ccgcactaac ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc
    17221 cgcacggttc aaaacggctg ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg
    17281 aaaatgcttc gtgatagcca atctgaacgc ccacctggcc ggggtattcc gctgccgccg
    17341 caaggaaacc ttcctgcagc accggatcgc ccgcgccgag tagcgccagc tgcccgccct
    17401 gctccagaag gcccggtaag gcttccagca ccaggtcgag acctttctgg ctggtcagac
    17461 ggctcaccac tgcaaaaagc ggcactttat catcaacctt aagccccatt gcgatttgta
    17521 actggcgctt attttccgct ttatcttcca acgtatcgcg ggtgtagcgc gaggccaaca
    17581 gtaagtccgt ctctggactc cagattttct cgtccacgcc gttcagtacg ccggaaagac
    17641 gcccttcacg gtgacgctgt tgcaacagac cttccatacc gtaggcaaac tgcggttcgg
    17701 tgatctcgcg agcgtaggtt ggactaaccg ccgtaatgtg atcggcatag tacagaccgg
    17761 ccttcaggaa agagatttgt ccgttgaatt ccagcccatg aatattaaag aatgaccatg
    17821 gcaattggat gtcattcata tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca
    17881 cagtaaacac cgacttcgcc ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg
    17941 catgccagtc gtgcgcatgc accacatcag gacgccagaa tgggtcaagc ccgctggcca
    18001 tttctgcccc aacccacccc agcagcgcaa aacgcaatac gttgtcggta taggcaaata
    18061 agttggtatc gtgatacggg cttcccggac gatcatagag atgcggcgcg tcaatcaggt
    18121 atatgccaac cccgttgtaa tgaccgaaca acagcgtgat atgtccggcg aaggtatcac
    18181 gacgggatac tacctgcgca tcggtaacgc cacggcgaat atcgggaaat gcaggcaaca
    18241 gtacgcgagc gtcaacgccg tctgcgattt gtgctgcggg taatgcccca ataacatcag
    18301 ccagaccgcc ggttttaagc agcgggaaca tctctgaaca tacatgtaaa acctgcatta
    18361 tcgctcctgt ttatgcccta acttccgtag catttcgcgc gttaccagca cgatgccttc
    18421 ttctgaacga tagaaacgac gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc
    18481 cggaataaca caagcacgat cgatgacgca gcggcgcaga cggcacgagc gacctaccca
    18541 tacttccggt aacaatacgg cggaatcaat gttgcagaat gaattcacgc gaacgcgcga
    18601 gaacagaacg gactgcacca ccaccgaacc ggagatcaca cagccgccgg ataccagtga
    18661 gttaagggtc atcccgtggc taccggagcg atcctgcacg aatttcgctg gcggtaatga
    18721 ttcattgtag gtgcgaattg gccaattgcg atcgtacata tccagttccg gcaccacaga
    18781 ggccagatcg aggttcgctt tccagtaagc ttccagcgta cccacatcgc gccagtacgg
    18841 ctcggcatcc gggtcggatt gtacgcaaga gagcgggaac gggtgcgcat aggccagacc
    18901 ggcttcggtg atcttgggaa tcaaatcttt gccaaagtcg tggctggagt tctcatcacg
    18961 atcgtcttct tccagcagtt catacagata gtcggcgtca aagacgtaga tacccatact
    19021 cgccagagat ttgctcggat cgttcggcat tgacggtggg ttagcaggtt tttcaacgaa
    19081 ttcgataatt ttatcgttct catcaaccgc cataacgcca aatgcggagg cttcttcaat
    19141 cggtactggc atacaagcaa cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat
    19201 acgcgagtag tcttgcttgt agatatggtc gcccgccagg atcaccacgt attccgcttt
    19261 ataacggcgg ataatgtcga ggttttgggt gaccgcatct gcggtgccgc gataccagtt
    19321 ttcccctttc attctctgct gtgctggcag cagatcgaca aactcgttca tttcttcatt
    19381 gaagaatgac cagccgcgct gaatgtgctg caccagagtg tgggactggt actgggtgat
    19441 cacgcccata cgacggatcc cggagttgat gcagttagac agcgcaaagt cgataatgcg
    19501 gaacttaccg ccgaagtgta cggccggttt tgctcgctta ttggttaaat ccttcaggcg
    19561 ggtaccacgt cctcccgcca gtatcagggc aacagatttc aatggcagct ggcgcgccaa
    19621 cattaagtga tcgttcttct ctaaactaac catgactaac tcctttttta tcatctctgg
    19681 aacacacaca atccgtgtgc aggtccctgc cagacagccg taatcactgg gttatcctct
    19741 ccagcgaatg ggggaatggc gtgccactcc ccagcaggta aaacaatctc tgttacctca
    19801 agcgtggcgt taattgcgat caaaaagcga tccgagagca gaatttgcag ctgtttcggc
    19861 ccgttttgcc actcatccgt gcttaaaggt tgagcatatc gatttagcca acggacattg
    19921 ccgtcgcctt cttcccacca gcgattctcc accaaagcgg gaatgcgctt gcgcagatgg
    19981 attaacgcgg cggtaaatgc ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt
    20041 aattggttat cctgacagta ggcattgtta ttgccatgct ggctgtgacc atgttcgtca
    20101 ccggccagta acatcggcgt accctgggag agcaacaacg ttgttaacag ggcgtgaatg
    20161 ctgtcgcgcc gccgttcaac caggtcaaga gaaccgccta acccttcttt accatgattg
    20221 ttactgtaat tgttgttggt cccgtcgcga ttttcttctc cgtttgcttc attgtgttta
    20281 tggttgaagc aaacgcagtc gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc
    20341 gcggcactcg gcagacgacc attacgttta aaaacatcgc tggaggcagc aaaacgcccg
    20401 gcaaacgccc ccagaggcaa atcataatgt agccagaaac gacgggcagc atcgcggaaa
    20461 tgatcgttcc actcggcaaa cagcggcggg aaatttccca cctgataacc accaggagcg
    20521 atatcccacg gttcagcaat taacttcacc tgcgagagca ccgggcagtt ctggatagcg
    20581 gtaaacaacg gcgcatcctg acggaactct ggcgtacggc ccatgactgc cgccagatca
    20641 aagcggaaac catcgacgtg gcaggtttct acccaataac gcaggcaggc gctggcataa
    20701 tccaccaccg ccggatgact caaattgagc gtgttgccgc aaccggtcca gttgtgataa
    20761 tcgccgtctt ctcttatcca ataatagcta cggttatcga tcccacgcag cgagaataac
    20821 gggccgtcga ggtccagttc cgcactatgg ttgagcacga tatcaagaat gacttcgata
    20881 cccgctttat gcagtgcttt gattgcatcg cgaaactcat ccagcgccgt ttctggcgag
    20941 caggcatacg ccggatgcag cgcaaacatc gccaccgggt tgtaacccca gtagttactt
    21001 agccccatgc gttgcagacg tggttcactg gcaaactgcg ccactggcag cagttccagc
    21061 gcggtaatgc ccaattgttt caaatagttg atcatcaccg gatgcccgag ggctttataa
    21121 gtgccacgga tctcgaccgg gatctccggg tgcaagtacg ttaatccttt gacatgggct
    21181 tcataaatga tggtgctgcc ccacggcgtg cgcggcgggg catcatcttc ccagtcatag
    21241 tgatcaacca ccactacgca tttcggcgca atggcggcgt tgtcgcgata gtcaggttca
    21301 ttatgaccgg cgtgcagcag cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa
    21361 ggatcaatca acaacttcgc cgggttaaag cgatgcccct cggcgggttg ccaggggcca
    21421 tgaacgcgat aaccataacg caaacccggg cgcgcatccg gcagataacc gtgccaaatg
    21481 tcgccactgt gccctggcaa gtcatagcga tgttcctggc cattggcgtc aaagacacac
    21541 agttctaccc gctcggcatg agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa
    21601 tgcgcgccga ggggagcggg tttgccaatg gcgagttgtg tcattctgcc tcccgaacca
    21661 gccagatagt ggccagcggt ggtagcgtca ggcttagtga atgctgacga ccgtggctgg
    21721 caatctcatc gctgtgtacc gtgccgccat tgcctgcatt actgccgtga tagtgcatgg
    21781 aatcggtatt gaggatttca cgccatttgc ccggctggtt tatgccgaag cgataatcat
    21841 gacgcggtac cggcgtaaag ttactggcaa cgatgatttc gttaccctct ttatcgcgac
    21901 gcacaaagat cagcaccgag cgttctttgt catccaccac cagccattca aagccgtacg
    21961 ggtcaaaatc cagttcatgc attgctttat ggtggcggta ggtgaggttc agatcgcgca
    22021 ccagacgctg gacaccgtgg tgccagttat cgccgccttc caacagatgc cagtcgaggc
    22081 tggcgtcatg gttccactcg cggccctggg caaattcgtt acccatgaac agtagtttct
    22141 tgcccgggaa tgcccacatc cagccatagt aggcgcgcag gttcgcgaat ttctgccatg
    22201 cgtcgcccgg catgcggtcg agaatcgatt ttttaccgtg gaccacttca tcatgcgaca
    22261 acggcaggac gaagttttca gtgtagttgt agagaatccc gaaggtcagt ttatcgtgat
    22321 gatactgacg ataaaccggg tcgagcttca tgtagtccag ggtgtcatgc atccagccga
    22381 ggttccactt gtaccagaag cccagaccgc ccatatcctg cggacgagaa acgccaggga
    22441 aatcggtaga ctcctcagcc attgtcaccg caccggaaac ctgctcacca agaatacggt
    22501 tggtattacg caagaattca atcgcttcaa gattctcgcg cccgccaaat tcgttcggga
    22561 tccactcccc ctctttacgg ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc
    22621 gcagcgcatc aataccaaaa cgttcaatcc agtaaagcgc gttaccgacg aggaagttac
    22681 tgacttcacg gcgaccatag ttgtagatca gcgtgttcca gtcctgatga tagccttcac
    22741 gcggatcgct gtgttcatac aagttcgtgc catcaaattc ggcaagcgca aagtcatcag
    22801 tcgggaagtg gcctggcacc cagtcgagaa tcacgttcag accagctgcg tgtgcggcat
    22861 caatgaaata acggaagtcg tcgcgagtac caaaacggcg ggttggtgca tacaggccgg
    22921 ttggctgata accccaactg ccatcgaagg gatgctcgtt aatgggcagt agttcgaggt
    22981 gggtaaagcc catccattta gcataaggca ccagttgatc ggccagctcg cggtagctca
    23041 accagaaatt gttgtcggtg tggcgacgcc aggaacccag gtgaacttca taaatagaga
    23101 ttggcgcatc aaactgattc gcttttttgc gctcttcagt ctgtacaacc ttttccggca
    23161 gcccgcaaat aagagacgcg gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg
    23221 acttcagacg caagttgcca ttggcatcaa tcatctcgta tttatagagc tgaccgttat
    23281 gcgccccagg gataaacagt tcccagatgc cgctctcttt acgcaggcgc atcgggtgac
    23341 ggcgaccgtc ccagtagttg aattgcccaa ccaccgagac ccgacgggcg tttggagccc
    23401 agacagagaa acgcgtacct gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt
    23461 catacgggcg caggtgagta ccttcagata atagccaggc atccatttcc tggattagcg
    23521 gaccaaaacg gtaaggatca tcaatcaggt tttgctgacc atgccagaca acagccaact
    23581 gatagcggaa aaaattctta cgtcgcggaa tgacgccgct aaagaatccc cgtgagtcga
    23641 gacactccag ttttgcgagt ttgcgcccgg ttttcggttc aatcacccac acatcggtag
    23701 cgtcgggtaa aagggcacgg acttccagtc ccgcggtggt tttatgcatt cccagtacgg
    23761 aaaaaggatc cgcaaaatgg cctgcaatta gcgcgttaat cacgtctcta tcgatacgat
    23821 cggacatgct tgtcttcctg ttttattgtg tcaccccatc caattggatt tttgacttct
    23881 ggttgtgaca tttttttgac ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc
    23941 ctcacttcag gtaaggctgt gaatactcat gtattcagcc acccttaaag aatagccaat
    24001 gctctattta actcccggta aatcatgaaa catctgcgct tactcctgta ttacgcacta
    24061 acaggggcgg catcgcgccc cagatttaat gaataaagat tacgccagtt gacgaagcat
    24121 ccgacgcagc ggctccgcgg ccccccacag cagctggtcg cccacggtaa aggctgacag
    24181 gaactctggt cccatattca gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt
    24241 aacggcagct ggggttagct cacgcatagt gatttcccga tcgttcggaa cgactttcgc
    24301 ccacggattg tgcgcagcca gcagttcttc cacggtcgga atagacacat cttttttcaa
    24361 tttaatagtg aatgcctggc tgtggcagcg caatgccccg acacgcacac ataaaccatc
    24421 taccggaatt acggaagatg tgttgaggat cttgttggtt tccgcctgcc ctttccactc
    24481 ttcgcggctc tgaccgttat cgagctgttt gtcgatccac ggaatcaggc tacccgccag
    24541 cggcacgcca aagttatcca ccggcagctc accgctacgg gttaaggttg tgactttgcg
    24601 ttcgatatcg agaatagcag aggacggggt cgcgagttca tctgccacat ggccatacag
    24661 atggcccatc tgggttaata actcacgcat atgtcgcgca ccaccgccgg aagcggcctg
    24721 gtaggttgca acggacaccc aatcaacaag atcattggcg aataaaccac ccaacgacat
    24781 caacatcagg cttacggtac agttaccgcc aacaaaagtc ctgatgccat tatttaatcc
    24841 gtcggtaatg acgtcctgat tgacggggtc aagaatgatg atggcgtcat ctttcatgcg
    24901 cagagacgat gctgcgtcaa tccagtaacc ttgccatccg ctttcacgaa gctttggata
    24961 gatttcgttg gtataatcgc cgccctgaca ggtcacaatg atatcgaggg cctttagcgc
    25021 ctccagatca aaggcatcct gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg
    25081 gccaagctga gaagtagaaa agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac
    25141 catgcgttgc atgagaacgg agccgaccat accgcgccag ccgataaaac caacattttt
    25201 cataagcgtt ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcttggca
    25261 catctttcac catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa
    25321 gccatcagta atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc
    25381 agggaatttg agttatgaat gaaatcattt ctgcagcagt tttattgatc ctgattatgg
    25441 atccgctcgg aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac
    25501 ggcgggcaat catggtgcga gagttgctta ttgctctcct ggtgatgctg gtgttcctgt
    25561 ttgcgggtga gaaaattctg gcatttctca gcctgcgagc agaaaccgtc tccatttctg
    25621 gcggcatcat tctgtttcta atcgccatta agatgatttt ccccagcgct tcaggaaata
    25681 gcagcgggct tccggcaggt gaagagccat ttatcgtgcc gttggctatt ccgttagtcg
    25741 ccgggccgac tattctcgcc acgctgatgt tgctgtctca ccagtaccca aatcagatgg
    25801 gacatctggt gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt
    25861 cttcgctatt tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg
    25921 gattgattct ggtgatgatg gcaacccaga tgttcctcga cggcattcga atgtggatga
    25981 aggggtaacg aggagagttt ttcgaaaaga acccggtagc gtcacaacta ccgggcgaac
    26041 aaacttaact caacagctgg aacgcaatca tcccaacgat ggcaccgaca gtgccgagga
    26101 tggtttccat catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac
    26161 cgaacaacca gaaaccggcg tcgttaacgt ggctgacaac aatcgaacca ccagcgatac
    26221 aaatcgacag cgccgccatt tgcgcaccgg agtagttcag ttgttcaata accggcatca
    26281 ccagtcctac cgccgttaaa caggctacgg tggcagaacc ctgaatgatg cgcactgcag
    26341 cggccagcac gaagcaggtg atggctatcg gcaggcccat gccagttaac gcttcgccca
    26401 gtgccggacc tacgccagag tcaaccagca cctgtttgaa cacgccacct gcaccaatca
    26461 ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca aatctccatc actttgtctt
    26521 ttggcatgcc ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg
    26581 cggtaaacgg atgaccaata aactcgaacc attcgtaagc ggtagagcct tctggcacaa
    26641 aacgcgcggc aatggttttc agccccacca gcaccagcgg caacaggatc agcgacaggc
    26701 tgaatccgaa agatggcatt ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa
    26761 tatgcaactc cacgtagcgg ctgatgaaat taccccacag cggcccggca ataatcattc
    26821 ccggaattgc cgcacacagg ccaatcagga tcatccagcc aaaatcggcg ttcatctgcg
    26881 atgccagcag cattggcgct ggccctggca ccaggaacgc cgccgctgcc gccacgcctg
    26941 cgaataatgg gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag
    27001 caacgctaat cagcagaaca atcgccactt caaagaacag cggtagcgca cagaccagcc
    27061 ccgcaaggcc gatggcataa tgcgcgcggc tgtgaccgaa ggatttaagc attttgacgg
    27121 caatctgatc gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga
    27181 caaccaccgc caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt
    27241 tatcgagcgg cataccggaa aaaaggccag cccccatgga caccaccatc aaagccagga
    27301 aagcgtgcat acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg
    27361 ttaaaacaag cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc
    27421 ttgccacaac accttccagc ggttgatcga tatccaccac cagtacatca gtttcgtccg
    27481 cacccggctc ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc
    27541 ctttgcgcgc tttcaggcgg ctttcaatca cgtcaaaatc gcctttcagg tagatgaaag
    27601 agagattcgg attaccttca cgcagcaagt cgcgatagtg ttttttcaat gcagaacaga
    27661 cgatcagtga cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca
    27721 accacggttt gcgatcgtcg tcattcagcg gttcgccaga cgccattttt tcgatattgc
    27781 gccgtggatg gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac
    27841 tggcgaccgc agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat
    27901 ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt tacgggtaac
    27961 tgttatcggt aacattgtcc agccggacaa tatcagaagc aatatccatc cgtgccttaa
    28021 gtgtataagt gtgagctact tcaaatttgt aggcttaaat agatccgccc ggtgacaagg
    28081 tgaaacctaa atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc
    28141 gttcagcgcc aatactgccc atccgctcac gcggcgtcag cacgctcgct aaccgcggct
    28201 ccatcacctg gccaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaacct
    28261 tcaatcccag acgctgacat tcaaacgccg cgccgaccgc caggtcatcg ttggtacaga
    28321 acacgccatc cagctgcgga tattcccgcc gcgcctggcg aatcagttca ataccggaag
    28381 agtaagaaga agattgctca accatcacgc tatacggcac cagacctgca tccagcatcg
    28441 cctgttcgta tcccttctgt ttgatgatag tacgttcgtc gagacgcgcg ccgagatagg
    28501 caatgtggca atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt
    28561 tatcaaaacc gacggcgata tcgaggcacg gcgactggct gtccatgagt tccaccaccg
    28621 ggatccccgc cacttcaatc atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga
    28681 tcagtccgtc gatattccat gagagcatgg attcgagacg ttcttgctcc atttccggtt
    28741 tataaccgta gtgcgccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc
    28801 cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc
    28861 ggctggtggc gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa
    28921 gagcggcggc aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac
    28981 ggctgaccgt cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt
    29041 tctttttcat cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca
    29101 tcagaacctt cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa
    29161 ttgcatcgca ttgtgctaac gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc
    29221 ggagcgagcg aggaagcctg caaaaaggca ggcttcaaaa atgccgtgga gctttaaacc
    29281 ggcggcagat cgaacagtaa cacttcgctg tcgctatccg catggatgga gattgcctgc
    29341 tcatcccaga ttgccagacc atcgctggtc gaggctttca cgccgttaat ggtgacattg
    29401 cctttcacca cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca
    29461 tctttcagca acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatct
    29521 cgcgcatccg gcgagagcac cagctgtttg ccctgtacgg catcgaagcg acgctgttca
    29581 taacgcggcg taataccgtt ttcttcaggc atgatccaga tctgatacag atgcagacgc
    29641 tcggtgctgc ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg
    29701 aactcacccg ccggaacctg ctctttattg cccatgctgt cctgatgctc aacagtacct
    29761 tccagcacgt aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct
    29821 gcttcaatca cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg
    29881 taatagttgg caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca
    29941 cgttcatttg ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg
    30001 cttagtgtgg acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa
    30061 aaaaattgaa ctattcgagg ggccatcaaa cttaattcgc gagagtcact gtggagggag
    30121 aggcttgctc aaatccgcgt tcaaggattt ccagattggt aagaacttca gattccttga
    30181 cgtaatttgg cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat
    30241 cgcccatctc cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca
    30301 gcacaccgac cgaattatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca
    30361 ggctggtttc ctgctggtcg ataccgtatt taataaacga acctttctta ccgtgaacga
    30421 taaatttcgg ataatcgatt ttcaccagat ggctggtttt gacgatggct ttcaggtcgc
    30481 cataaaacag ttgcgcttca aaggtgtcgt caggattggc tttattacgc aggctgcgga
    30541 tgtcataagc gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca
    30601 caccaaggcc ataaaacgcg ccatcctgcg gcagcccagg tttggtttct gccaccgggc
    30661 ggtaatagtc aaaatggctt tccacttcaa caatctctcc caacttgcca ctttcaatcg
    30721 cttttttcgc tgtcaggaag caggagtcaa agcgacgatt ctgatacggc gtgacggtca
    30781 gccctttgct tttcgccaac gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg
    30841 gtttttcgac cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc
    30901 tgtccgcgtg ggtgcagaca acaaccagct taacatcggg atcgtttagt acttcgtcga
    30961 gatcgctggt gaaatggata tgggaataaa tgggagcctg ttcttccggc ttcgcatggc
    31021 gacgaaaaat atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt
    31081 aacgggtggt gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct
    31141 ttttaaggtg gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca
    31201 taacgaacac aagcactccc gtggataaat tgagaacgaa agatcaaaaa aaaagccagc
    31261 acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc
    31321 gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact tgtccaacac
    31381 tttttgcaaa aaaatgcatt tgactcgcat ttgaaagtca atgatgttga aagggacatt
    31441 taccccaaag aggacaaagg aatgagtgag atagtaatac gccacgcaga aacacgggat
    31501 tacgaggcca tcaggcagat tcacgcccag ccggaggtgt attgcaacac actacaggtg
    31561 cctcatcctt ccgatcatat gtggcaggag cgactcgccg atcgtcccgg catcaagcaa
    31621 ctcgtcgcct gtattgatgg agacgtcgtg ggccatctca ccattgacgt gcaacagcgc
    31681 ccacgccgca gtcatgttgc cgattttggt atctgtgtcg actctcgttg gaagaaccgc
    31741 ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt gcgacaactg gttgcgggta
    31801 gatcgcattg aactaaccgt gtttgtcgat aacgccccgg caattaaggt ctataaaaaa
    31861 tacggctttg aaattgaagg gactggtaag aagtacgcat tgcgtaatgg tgaatatgtc
    31921 gatgcatatt atatggcgcg ggtgaagtaa gatagtgccc tttttctgag atggaaaaag
    31981 ggtgtcattc aaaatcgaca taccttcctt taaggtattt a
//
LOCUS       DAZFRJ010000035        31960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000035 DAZFRJ010000000
VERSION     DAZFRJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.035"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..515
                     /gene="cmtB"
                     /locus_tag="ACHKUN_001468"
     CDS             72..515
                     /gene="cmtB"
                     /locus_tag="ACHKUN_001468"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO6659760.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            543..1931
                     /gene="cmtA"
                     /locus_tag="ACHKUN_001469"
     CDS             543..1931
                     /gene="cmtA"
                     /locus_tag="ACHKUN_001469"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO6659761.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1946..3223
                     /gene="yggP"
                     /locus_tag="ACHKUN_001470"
     CDS             1946..3223
                     /gene="yggP"
                     /locus_tag="ACHKUN_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6659762.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3220..4185
                     /gene="glpX"
                     /locus_tag="ACHKUN_001471"
     CDS             3220..4185
                     /gene="glpX"
                     /locus_tag="ACHKUN_001471"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6659763.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4207..4716
                     /gene="fumE"
                     /locus_tag="ACHKUN_001472"
     CDS             4207..4716
                     /gene="fumE"
                     /locus_tag="ACHKUN_001472"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO6659764.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4713..5426
                     /locus_tag="ACHKUN_001473"
     CDS             4713..5426
                     /locus_tag="ACHKUN_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO6659765.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5398..6075)
                     /locus_tag="ACHKUN_001474"
     CDS             complement(5398..6075)
                     /locus_tag="ACHKUN_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6659766.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6069..6746)
                     /locus_tag="ACHKUN_001475"
     CDS             complement(6069..6746)
                     /locus_tag="ACHKUN_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6659767.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6734..7441)
                     /locus_tag="ACHKUN_001476"
     CDS             complement(6734..7441)
                     /locus_tag="ACHKUN_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO6659768.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7442..8020)
                     /locus_tag="ACHKUN_001477"
     CDS             complement(7442..8020)
                     /locus_tag="ACHKUN_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO6659769.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8043..8474)
                     /locus_tag="ACHKUN_001478"
     CDS             complement(8043..8474)
                     /locus_tag="ACHKUN_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO6659770.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8846..9865
                     /gene="epd"
                     /locus_tag="ACHKUN_001479"
     CDS             8846..9865
                     /gene="epd"
                     /locus_tag="ACHKUN_001479"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO6659771.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9915..11078
                     /gene="pgk"
                     /locus_tag="ACHKUN_001480"
     CDS             9915..11078
                     /gene="pgk"
                     /locus_tag="ACHKUN_001480"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO6659772.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11293..12372
                     /gene="fbaA"
                     /locus_tag="ACHKUN_001481"
     CDS             11293..12372
                     /gene="fbaA"
                     /locus_tag="ACHKUN_001481"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6659773.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12436..12534)
                     /locus_tag="ACHKUN_001482"
                     /pseudo
     CDS             complement(12436..12534)
                     /locus_tag="ACHKUN_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12730..13590
                     /gene="mscS"
                     /locus_tag="ACHKUN_001483"
     CDS             12730..13590
                     /gene="mscS"
                     /locus_tag="ACHKUN_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO6659774.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13729..14364
                     /gene="argO"
                     /locus_tag="ACHKUN_001484"
     CDS             13729..14364
                     /gene="argO"
                     /locus_tag="ACHKUN_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO6659775.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14457..15197
                     /gene="yggE"
                     /locus_tag="ACHKUN_001485"
     CDS             14457..15197
                     /gene="yggE"
                     /locus_tag="ACHKUN_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO6659776.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15364..16260
                     /gene="ygfI"
                     /locus_tag="ACHKUN_001486"
     CDS             15364..16260
                     /gene="ygfI"
                     /locus_tag="ACHKUN_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6659777.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16257..17735)
                     /gene="scpC"
                     /locus_tag="ACHKUN_001487"
     CDS             complement(16257..17735)
                     /gene="scpC"
                     /locus_tag="ACHKUN_001487"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO6659778.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17759..18544)
                     /gene="scpB"
                     /locus_tag="ACHKUN_001488"
     CDS             complement(17759..18544)
                     /gene="scpB"
                     /locus_tag="ACHKUN_001488"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO6659779.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAQLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18555..19550)
                     /gene="meaB"
                     /locus_tag="ACHKUN_001489"
     CDS             complement(18555..19550)
                     /gene="meaB"
                     /locus_tag="ACHKUN_001489"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO6659780.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19543..21687)
                     /gene="scpA"
                     /locus_tag="ACHKUN_001490"
     CDS             complement(19543..21687)
                     /gene="scpA"
                     /locus_tag="ACHKUN_001490"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO6659781.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21891..22784)
                     /gene="argP"
                     /locus_tag="ACHKUN_001491"
     CDS             complement(21891..22784)
                     /gene="argP"
                     /locus_tag="ACHKUN_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO6659782.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22926..23111
                     /locus_tag="ACHKUN_001492"
     CDS             22926..23111
                     /locus_tag="ACHKUN_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659783.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23167..23826
                     /gene="rpiA"
                     /locus_tag="ACHKUN_001493"
     CDS             23167..23826
                     /gene="rpiA"
                     /locus_tag="ACHKUN_001493"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO6659784.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24082..25314
                     /gene="serA"
                     /locus_tag="ACHKUN_001494"
     CDS             24082..25314
                     /gene="serA"
                     /locus_tag="ACHKUN_001494"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6659785.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25543..25602
                     /gene="ibsC"
                     /locus_tag="ACHKUN_001495"
     CDS             25543..25602
                     /gene="ibsC"
                     /locus_tag="ACHKUN_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO6659786.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25935..25994
                     /locus_tag="ACHKUN_001496"
     CDS             25935..25994
                     /locus_tag="ACHKUN_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6659787.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26095..26697)
                     /gene="fau"
                     /locus_tag="ACHKUN_001497"
     CDS             complement(26095..26697)
                     /gene="fau"
                     /locus_tag="ACHKUN_001497"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO6659788.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26718..26901)
                     /gene="ssrS"
                     /locus_tag="ACHKUN_001498"
     ncRNA           complement(26718..26901)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKUN_001498"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26943..27272)
                     /gene="zapA"
                     /locus_tag="ACHKUN_001499"
     CDS             complement(26943..27272)
                     /gene="zapA"
                     /locus_tag="ACHKUN_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6659789.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27440..28018
                     /gene="ygfB"
                     /locus_tag="ACHKUN_001500"
     CDS             27440..28018
                     /gene="ygfB"
                     /locus_tag="ACHKUN_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO6659790.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28044..29369
                     /gene="pepP"
                     /locus_tag="ACHKUN_001501"
     CDS             28044..29369
                     /gene="pepP"
                     /locus_tag="ACHKUN_001501"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO6659791.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29366..30544
                     /gene="ubiH"
                     /locus_tag="ACHKUN_001502"
                     /gene_synonym="visB"
     CDS             29366..30544
                     /gene="ubiH"
                     /locus_tag="ACHKUN_001502"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO6659792.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30567..31769
                     /gene="ubiI"
                     /locus_tag="ACHKUN_001503"
                     /gene_synonym="visC"
     CDS             30567..31769
                     /gene="ubiI"
                     /locus_tag="ACHKUN_001503"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO6659793.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31843..31926)
                     /locus_tag="ACHKUN_001504"
     CDS             complement(31843..31926)
                     /locus_tag="ACHKUN_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659794.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT         7646 a         7917 c         8605 g         7792 t
ORIGIN      
        1 tttcattaaa ccgcacgagt attttatttt taccgccgga cggtacccta aaaataaaat
       61 aaggataatt tatgcggctt agtgattatt ttccagaatc atcaatctca gttatacatt
      121 cagcaaaaga ttggcaggaa gctatcgatt tctcgatggt atcattgctg gataaaaact
      181 atatcagcga gaattacatt caggcaatta aagattccac cattaacaat ggcccttatt
      241 atattctcgc accaggcgtg gcaatgcctc atgcgcgacc ggaatgtggg gcgcttaaaa
      301 ccgggatgtc tttgacatta cttgaacaag gtgtttattt tccggggaat gacgaaccaa
      361 ttaaattact catcggactc tctgctgccg atgccgattc gcacattggc gctattcagg
      421 cgttaagtga attactgtgc gaagaagaaa tactcgaaca actcttaaca gcatcatcag
      481 aaaaacaatt agcggacatt atcagccgcg gataattact ctctcctcta caggataata
      541 ttatggaaaa caagtctgct cgtgcaaagg tccaggcttt tgggggcttt ttgactgcaa
      601 tggtcatccc caatattggt gcttttattg cctggggttt tattactgcg ttatttattc
      661 ccaccggttg gctgcctaac gaacatttcg ccaaaattgt cggcccgatg attacctatt
      721 tattgcccgt gatgattggt tctacaggtg gtcatctggt cggcggtaaa cgcggggcgg
      781 tcatgggcgg aataggtact attggtgtga tcgttggcgc agagatcccg atgttccttg
      841 gctcaatgat catggggccg ctcggtgggt tggtcataaa atatgtcgat aaggcactgg
      901 aaaaacgcat acctgccggt tttgagatgg ttatcaataa cttctcatta ggtatcgcgg
      961 ggatgctcct ttgtctgctg ggttttgaag tgatcggccc ggcggtgtta attgccaata
     1021 ctttcgtcaa agagtgtatc gaggcgctgg tacatgcggg ttatctgcct ctgctgtcag
     1081 tcatcaatga accggcgaaa gtgcttttcc ttaataatgc gatcgatcag ggcgtctatt
     1141 atccgctggg aatgcaacag gcttcggtta acggtaaatc catcttcttt atggtggcct
     1201 ctaacccagg tccgggcctg gggctgctgc tggcgtttac cttgtttggt aaagggatga
     1261 gtaaacgttc tgcgcccggg gcgatgatta ttcacttcct cggtgggatc cacgaactgt
     1321 atttcccata tgtgctgatg aagccgctga ctattattgc catgattgcg ggcggtatgt
     1381 ctggtacctg gatgtttaac ttactggacg gtggtctggt ggctggccca agtccggggt
     1441 ctatctttgc ttacctggca ctgacgccga aaggctcgtt cctggcgaca attgccggtg
     1501 ttacggtagg taccctggtg tcctttgcta ttacttcgct gatactgaag atggaaaaaa
     1561 cggtggaaac ggagagcgaa gatgagtttg ctcagtcagc caatgcggtt aaggcgatga
     1621 aacaagaggg tgcattctcg ttaagcaggg ttaagcgtat cgcctttgtt tgcgatgcgg
     1681 ggatgggctc cagtgcgatg ggcgcgacca ccttccgtaa acgcctggaa aaagcggggc
     1741 tggcaataga agtaaaacat tacgcgatag aaaacgtgcc tgcggatgcc gatatcgtcg
     1801 tcactcacgc cagtctggaa gggcgcgtga aacgtgtgac ggataaaccg ctgatattga
     1861 ttaataacta tattggcgat ccaaaactcg acactttatt taatcaatta accgccgaac
     1921 ataaacactg attacagagg taaaaatgaa aaccaaagtt gctgctattt atggcaagcg
     1981 ggatgtccgt ctgcgcgaat ttgaactgcc agaaattacc gataatgaac tgttagtgag
     2041 tgtaatttct gacagcgtct gtttatcgac ctggaaagcg gcgttactcg gtagtgaaca
     2101 taaacgcgta cccgacgatt tagaaaatca tccggtcatt accgggcatg aatgtgccgg
     2161 ggttattgtc gaagtgggta aaaatctcac tggcaaatat aaaaaaggcc agcgttttgt
     2221 attgcaaccg gcgatggggt taccaagcgg atattcagca ggctacagct acgaatattt
     2281 tggcggtaac gccacctata tgattattcc cgaaatcgcc attaatttgg gctgcgtatt
     2341 accgtatcac ggctcttatt ttgctgcggc gtcgctggca gagcctatgt gctgcattat
     2401 tggtgcttat catgccaatt atcacaccac gcaatatgtt tatgagcatc gcatgggcgt
     2461 caaacctggc ggcaatattg cactgctggc gtgtgcgggt ccgatgggca ttggcgctat
     2521 cgattacgcc attaacggtg gcatacaacc gtcacgggtg gtggtggtcg atatcgacga
     2581 caaacgcctg gcgcaggtgc agaagctgct gccggtggat ctggcggcca gtaaaggcat
     2641 tgagctggtg tatgtgaata ccaaagggat gagcgatccg gtccagacgc tgcgggcgct
     2701 gacaggagat gtcgggttcg atgacatttt tgtttatgcg gcggtgcctg ctgtcgttga
     2761 gatggctgac gaattactgg cggaagatgg ctgtctgaac ttctttgccg ggccgacgga
     2821 taaaaacttc aaagtgccgt ttaatttcta caacgtccat tacaatagca cgcacgtagt
     2881 cggtacatcc ggcggttcaa cggacgacat gaaagaggcg attgcgttaa gcgccacggg
     2941 gcagttgcag ccgtccttta tggtcacgca tatcggtggg ctggatgcgg tgccagatac
     3001 cgtgctcaat ctgccggata tccctggcgg taaaaaactc atttataacg gcgtgaccat
     3061 gccgctcact gccattgccg attttgccga aaaaggcaaa accgatccgc tgtttaaaga
     3121 gttggcgcgg ctggttgagg aaacgcacgg catctggaat gaacaggccg agaaatatct
     3181 gctggcacaa tttggcgttg atatcgggga ggccgcgcaa tgatgtccct ggcgtggcca
     3241 ttatttcgcg ttacggaaca ggcagcgttg gctgcctggc cgcaaaccgg atgtggcgac
     3301 aaaaataaaa ttgatggcct ggcggtcacc gcgatgcgcc aggcattaaa cgacgtcgct
     3361 tttcgtggac gagtggttat cggcgaagga gaaattgacc atgcgccgat gctgtggatt
     3421 ggcgaagagg tgggcaaagg cgatgggcca gaagtcgata tcgcggttga cccgattgaa
     3481 ggcacgcgga tggtagcgat ggggcagagc aatgcgctgg cggtcatggc tttcgcccct
     3541 cgagatagcc tgttacatgc gcccgatatg tatatgaaaa agctggttgt taatcgactg
     3601 gctgccgggg cgattgatct gtctttgcct ctggcggaca acttgcgtaa cgtggcgcgg
     3661 gcgttaggta aaccgctgga caagctgcgc atggttacgc tggataaacc gcgcctgagt
     3721 gccgcgattg aagaagcaac acagctgggc gtgaaggttt tcgctctgcc ggatggcgat
     3781 gtcgccgcca gcgtgctgac ctgctggcag gataatcctt acgacgtgat gtacaccatc
     3841 ggcggtgcgc cagaaggcgt gatttctgcc tgtgccgtta aagcattagg tggggatatg
     3901 caggccgagc tgattgactt ttgtcaggct aaaggggatt acacggaaaa tcggcagatt
     3961 gcagagcagg agcgcaagcg ttgtaaggca atgggcgttg acgttaaccg tgtgtactcg
     4021 ctcgatgaac tggtgagggg gaacgatatc ctctttagcg ctacgggcgt gacgggcggc
     4081 gagttggtga acggtatcca acagacggcg aatggggtgc ggacgcagac attactgatc
     4141 ggcggcgcgg accaaacgtg taatataata gactccctgc attgatggtg atctatcgga
     4201 agaccaatgg cgacgctgac agaagatgat gtgcttgagc aactggatgc acaggacaat
     4261 ttattttcat ttatgaaaac tgcgcattct attttgctcc aggggatacg ccagtttctg
     4321 ccgtcgctgt ttgtcgataa cgatgaagag atcgtcgaat atgcagtgaa gccgttactc
     4381 gcccaaagcg gcccgcttga cgatattgat gttgcgctgc gtttgattta tgcgctgggg
     4441 aaaatggata aatggctgta tgccgatatc acgcactttt cccagtactg gcattacctg
     4501 aacgaacagg atgaaacgcc cggatttgcc gatgacatga cctgggattt tatcagcaac
     4561 gtcaatagca taacctgcaa tgcgacgctc tatgacgcgt taaaagcgat gaagttcgcc
     4621 gatttcgccg tctggtcaga ggcgcgtttt agcggaatgg tcaaaacggc gctgacgctg
     4681 gcagtaacga ccaccttaaa ggaattaacg ccgtgaaaat tgaattaacg gtgaatgggc
     4741 tgaacgtgca agcgcagtac catgatgaag agatagaacg cgtgcataaa ccgttgctgc
     4801 gtatgctggc ggccttacag acggtaagtc cgcagcgacg tacggtggtt tttctttgcg
     4861 ctccgccggg gacgggtaaa tctaccttaa ctaccttctg ggaatatctc gcgcaacaag
     4921 accctgaatt acccgctatc cagacgctcc cgatggatgg ttttcaccat tacaatagct
     4981 ggctggatgc gcatcaattg cgccccttca aaggcgcacc agagacattt gacgttgcga
     5041 aactggcgga aaatctgtgc caggtagtgg aaggggattg tacgtggccg cagtatgatc
     5101 gacaaaagca tgatcctgtt gaagatgcgt tgcacgttac cgcaccactc gtcatcgtcg
     5161 aaggaaactg gttgttgctg gacgacgaga agtggtgcca gcttgcgcaa ttttgtgatt
     5221 tctcgatctt tatcaacgcg ccagctactg cgttgcggga acgactggta gggcgcaagc
     5281 tggcgggagg ggtatcgctg gcagacgcag aagcttttta tgaccgtacc gacgggccga
     5341 atgttcgccg ggggctggaa gagagtctgc ctgcaaattt gaccttaatg atgaccgcta
     5401 cgggtgaata tcgtctggtg gattaacgtt cctgattaga ccatcttcca gtcgcaccac
     5461 acgagagaaa tgacgccggg taaatgcggc gtcatgactg attgctacga cgctcgtgcc
     5521 tcgctggccg cacttctcca gccagctttc aaacacgctc aaccaatttt cgtcaaaatc
     5581 ccgactcggc tcgtccagca gcaatagtgg cggagagagt gcttccaggc aggcgacggc
     5641 gaccatccgt cgctgcgcac tatgcagatc caacgggtgc gagttggcag tgtctgctaa
     5701 ctgacaacat tgcagggcag catgggtacg ttgagtgatt tcatccgccg ggcatttttg
     5761 cagtttcaaa ccaaatgcga tctcgtcagc gacggtgctg tgaaaaagtt ggttttccgc
     5821 ttcctgaaac agtacgccaa cctttgccgc gcgctggcgg tttttcaggt ttttcatcgc
     5881 ctgttgttgc agcataaccg taccggctgt gggcgtaaga agacctgcca ttactcgtaa
     5941 cagcgtcgat tttcccgcgc cattgtcgcc ggtcagcgcc aaccattcac cttgtttgag
     6001 ttgcagagaa atgtcgcaaa gacaatccgt tgccgcgcca ggccagcgat aagatatctt
     6061 atttaacgtt aacatagtgg ctggagcgct ccgttttgca attgccacgc ttgctggcag
     6121 tagttcagga aaggtgtgcg atgacgttca aaaagaataa tcagcgaacc acgttcaaac
     6181 gcccagtgtt gtaagcgctg caacagcatt tcgctggcct gcggagtcag gcggctaaag
     6241 gcttcatcaa gaattaagag ctttggctgc atcgcgatgg cgcaggcgat gaccacgcgc
     6301 tgtgtctctc caccggagag tgttgccggg tgacgatggc gcaacggctg gcactcggtc
     6361 agagtcaggg cggcatcaat ccgcgccata atctctgctt cagcaagaca cagattttct
     6421 ggcccaaagg caacttcttc ttccacgcta aaggtacagc cggagagctg caaatagggc
     6481 gattgctgga cgagctggat ggtggcggat tgttcattaa gcgggagatg cccaatgggc
     6541 gtaccgagga gcgtccccgt gcctgttatc tctcctggca gaaagtccgg ataccagccc
     6601 gccatcagct gtgccagcgt acttttgcca ctgccgttgt caccaaagat ggcgaccatc
     6661 cccggtttga catagtgaaa gtcataacag aacggaggat gcgttgagtg tgtcgggcaa
     6721 taacggaact gttctaacgt aaccatatcc acgctccgaa ttctgtcagc atcagcaaga
     6781 tcatggtgta acgcgctacg cgttgtaggg tgctatcggc tggtgcccac aacgttgttc
     6841 gtcggttgtg gatccgaaat gcccgcatat ccagcgccgc accacggaca gccagatcgt
     6901 taagcgcatt gtgtgttagc ggaataatta acgcgggcat cgcacgtaaa cgctgatacc
     6961 agccctcatc cagcggcacg ccgcgggcac gctgcgcttc atgaataatc gctaattgcc
     7021 gttttaactg ctcaacgacc aataacggcc cggcaaacag ataagcgacg cctggcggca
     7081 ggcgagaagc aaacagagcg cgaataaaac attgcacggg gacgtactgc atccacaact
     7141 gtgaggttga aacaatcgcc agaatccgca accagagcgt aatagcatgc gaccaccgtt
     7201 ctggcgagcg tggcgtgccg cttagccatt ctggtagcca gccaccgtgt accagccaca
     7261 gcccggctcc cagcgagaac atcagccaga cgacatattt tgcccgccgc tgcgtggcct
     7321 ttagcgcaat aaggcagaaa aaggtcgccg cgctatagat cggcaaaatg gtctgcgcgg
     7381 gcaggataag cgtggtgcag gccgccaatg cccacagtgt taatgaggta aatggatgca
     7441 tttagcgcac ggcagccatt gccggaaaat ggcgcgtggt gcgaatcggc agctggcgca
     7501 gtaaaagcca gacaatcacc gctgtaagaa ttttatcgac caggttcgcg cctatcaccg
     7561 taatcgccac cgactccacc aggttttgcc ccatcgagtg catccaggca acgaacaaat
     7621 cggctccgct accggtgaca ccgccgaaca gcgcagtacg cagcggtaca gcgacgaccg
     7681 ttaccgcaag cgtaataatc acgccgctga caacgacttt tggcagggtg cgaaaccaac
     7741 ccgctcgcgc caggcaaccg gcgaccaggc cgatgaccat cgcgacgggt gcaaatgcgg
     7801 cggcgatcgg gttggtcagc aatccccaga gtaaattggt cagtaagccg gtcagcattc
     7861 cgatcaccgg accaagcagt accgcgctaa taagtgtgcc gatggagtcg agaaatatag
     7921 gtaatttgac catgctggcc agttgcccgc cgatcatatt gatggcgata gatataacga
     7981 tcaacaccag tgcctgactg gaaaagtggc tacgcgccat aagaaaacct ctttgaaagt
     8041 agttatttaa cgggggaaat atgcaactcg tcgtaaccag aaagcgcgca aggttggcgg
     8101 ctaaacgcca gttcctcaag gctaccgatc accagttcaa gcgtggtccg cacggtacag
     8161 ccgggcatca tatgaccgcc aagcatcgtg ccgtgtggat cggaaacgca gagatgaaga
     8221 tgctcaccac tctgttccag cgtgccgttt agtgagatca cttcgaatgt accgttcagg
     8281 agcgtggtgc cttcttgccc ggcatagcgc aaggcgacgt cagtcaggct accggtacag
     8341 cctgcaatcc aggcggcatg aagttgatgc tgctgtgcaa aagcgtgtag ctgggaaaaa
     8401 acttcctgcc cggggaggag gcgcagtgcg taaaagcgtg cggaggaaga atatgggtag
     8461 ggcaatgtca tcattggagt actccgaaac tcggacgtat tgcggataat atcgacgctg
     8521 catcagtcat attctgatat aagtcagggt ttgtgacggc acaacgaaaa gattttgcta
     8581 cgcttattcg tgattctgca aagtggaata aattccacag gggcggggct gaaaacgtga
     8641 cgtaagtcag ataattgctt tctttgactg gacaatcatt ccttttattc cacgtttcgc
     8701 ttatcctagc tgaagcgttt cagtcgatta aatgttcgac aattaaccaa tcagtcgcag
     8761 tttgcgacag gtaaggtttc cccggacgat ttgctggatt actctgtctg gcaaatttgc
     8821 tcagggaaaa ccttgcagga gatctatgac cgtacgcgta gcgataaatg gcttcggtcg
     8881 catcgggcgt aatgtggttc gtgctttgta tgaatccgga cgccgggcgg aaattaccgt
     8941 ggtggcaatc aacgaactgg cggatgctgc gggcatggcg catttgttga aatatgacac
     9001 cagccatggc cgttttgcat gggaagtacg acaggaacgc gatcaacttt ttgttggtga
     9061 tgacgccatc cgcgtattgc atgaacgttc actgcaatcg ctcccctggc gtgaacttgg
     9121 cgttgatgta gtcctcgact gcaccggcgt atatggctcc cgcgagcatg gcgaagcgca
     9181 tattgccgcc ggggccaaaa aagtgctctt ttcacatcct ggcagtaacg atctcgacgc
     9241 gaccgttgtt tacggcgtca atcaggatca acttcgtgcg gaacaccgca tcgtttctaa
     9301 cgcttcctgt accacgaatt gcataattcc cgtcatcaaa ttgttagatg atgcgtacgg
     9361 tattgagtcc ggcactgtga ccacaattca ctccgccatg cacgatcaac aggttattga
     9421 tgcataccat cctgacctgc gtcgcacccg ggcagccagc cagtcgatca ttccggtcga
     9481 tactaaactg gccgccggta tcacacgatt ttttccgcaa tttaacgatc gctttgaagc
     9541 gattgcggta cgtgtgccaa ccataaatgt gacggcaatc gatttaagcg tgacggtgaa
     9601 gaaacctgta aaagccaatg aagtcaacct gttgctgcaa aaagcagcac aaggtgcatt
     9661 tcatggtata gttgactata cggaattgcc gttggtctct gtagatttta accacgatcc
     9721 gcacagtgcc attgtcgatg gcacccaaac ccgggtcagt ggcgcacacc tgatcaaaac
     9781 gttggtctgg tgcgataacg aatggggctt tgctaaccga atgctcgaca cgacgttagc
     9841 tatggctact gttgctttca ggtaagacgc aagcagcgtc tgcaaaactt ttagaatcaa
     9901 cgagaggatt caccatgtct gtaattaaga tgaccgatct ggatcttgct gggaaacgtg
     9961 tatttatccg tgcggatctg aacgtaccag taaaagacgg gaaagtaacc agcgacgcgc
    10021 gtatccgtgc ttctctgccg accatcgaac tggccctgaa acaaggcgca aaagtgatgg
    10081 taacttccca cctgggtcgt cctaccgaag gcgagtacaa cgaagaattc tctctgctgc
    10141 cggttgttaa ctacctgaaa gacaaactgt ctaacccggt tcgcctggtt aaagattacc
    10201 tcgacggcgt tgacgttgct gaaggtgaac tggttgttct ggaaaacgtt cgcttcaaca
    10261 aaggcgagaa gaaagacgac gaaaccctgt ccaaaaaata cgctgcactg tgtgacgtgt
    10321 tcgtaatgga cgcattcggt actgctcacc gcgcgcaggc ttctactcac ggtatcggta
    10381 aattcgctga cgttgcgtgc gcaggcccgc tgctggcagc tgaactggac gcgctgggta
    10441 aagcactgaa agaaccagct cgcccgatgg tggctatcgt tggtggttct aaagtatcta
    10501 ccaaactgac cgttctggac tccttgtcta aaatcgctga ccagctgatt gttggtggtg
    10561 gtatcgctaa cacctttatc gcggcacaag gccacgatgt gggtaaatcc ctgtacgaag
    10621 ctgacctggt tgacgaagct aaacgtctgc tgaccacctg caacatcccg gttccgtctg
    10681 atgttcgcgt agcaaccgag ttctctgaaa ctgcaccggc taccctgaaa tctgttaacg
    10741 atgtgaaagc tgacgagcag atcctggata tcggtgatgc ttccgctcag gaactggctg
    10801 aaatcctgaa gaatgcgaaa accattctgt ggaacggtcc ggttggcgtg ttcgaattcc
    10861 cgaacttccg caaaggtact gaaatcgtgg ctaacgctat cgcagacagc gaagcgttct
    10921 ccatcgctgg cggcggcgac actctggcag caatcgacct gttcggcatt gctgacaaaa
    10981 tctcctacat ctccactggc ggcggcgcat tcctcgaatt cgtggaaggt aaagtactgc
    11041 ctgcagtagc gatgctcgaa gagcgcgcta agaagtaaaa aatcacaggg cagggaaacc
    11101 tgcccttgtt tcagcgcgct tttagagcac gcattttttc atactctaaa taattcgagt
    11161 tgcaggaagg cgacaagaga gtgaatcccc aggagcttac ataagtaagt gactggggtg
    11221 agcgaacgca gacgcagcac atgcaacttg aagtatgatg agtataaggc ccgacgatac
    11281 aggacaagag acatgtctaa gatttttgat ttcgtaaaac ctggcgtaat cactggtgat
    11341 gacgtacaga aagttttcca ggtagcaaaa gaaaacaact tcgcactgcc agcagtaaac
    11401 tgcgtcggta ctgactccat caacgccgta ctggaaactg ctgctaaagt taaagcgccg
    11461 gttatcgttc agttctccaa cggtggtgct tcctttatcg ctggtaaagg cgtgaaatct
    11521 gacgttccgc agggtgctgc tatcctgggc gcgatctctg gtgcgcatca cgttcaccag
    11581 atggctgaac attatggtgt tccggttatc ctgcacactg accactgcgc gaagaaactg
    11641 ctgccgtgga tcgacggtct gctggacgcg ggtgaaaaac acttcgcagc taccggtaag
    11701 ccgctgttct cttctcacat gatcgacctg tctgaagaat ctctgcaaga gaacatcgaa
    11761 atctgctcta aatacctgga gcgtatgtcc aaaatcggca tgactctgga aatcgaactg
    11821 ggttgcaccg gtggtgaaga agacggcgtg gacaacagcc acatggacgc ttctgcactg
    11881 tacacccagc cggaagacgt tgattacgca tacaccgagc tgagcaaaat cagcccgcgt
    11941 ttcaccatcg ctgcgtcctt cggtaacgta cacggtgttt acaagccggg taacgtggtt
    12001 ctgactccga ccatcctgcg tgattctcag gaatatgttt ctaagaaaca caacctgccg
    12061 cacaacagcc tgaacttcgt attccacggt ggttccggtt ctactgctca ggaaatcaaa
    12121 gactccgtaa gctacggcgt agtgaaaatg aacatcgata ccgataccca atgggcaacc
    12181 tgggaaggcg ttctgaacta ctacaaagcg aacgaagctt atctgcaggg tcagctgggt
    12241 aacccgaaag gcgaagatca gccgaacaag aaatactacg atccgcgcgt atggctgcgt
    12301 gccggtcaga cttcgatgat cgctcgtctg gaaaaagcat tccaggaact gaacgcgatc
    12361 gacgttctgt aagtcattcc tttctgctta tctcaaggcc cgctctgcgg gtcttttttt
    12421 cgccaaaagc aaaattgcct gatgcgctac gcttatcagg cctacacgta tcctgcaata
    12481 tattgaattt gcaggttttt gtaggccgga taaggcgttc acgccgcatc cggcatgaca
    12541 aagcgcacat tgatatctgt ctgagccccg ctctgcgggg attttttcgc caaaagcaag
    12601 gtgattcaga tgagaaatct gtgatctatt tggcaaaatt atgctttatt gtttaccctt
    12661 gtcagactgc ccgtcataag gcggcggagt gtatttctcc attttgagtc agttgaaaag
    12721 gaatattgaa tggaagattt gaatgttgtc gatagcataa acggcgcggg aagctggctg
    12781 gtagctaacc aggcgctgct gctaagttat gcagtaaaca tcgtggcggc actcgcgatc
    12841 atcatcgttg gtttgattat cgcgcggatg atttccaacg cggtgaatcg cctgatgatc
    12901 tcccgtaaaa tcgatgccac tgttgctgat tttctttctg cattagtccg ttacggtatt
    12961 atcgccttta cgctaatcgc agccctggga cgcgtgggtg tacaaacagc gtcagtcatt
    13021 gctgtactcg gtgccgcagg cttagctgtt ggtctggctt tgcaggggtc actttctaac
    13081 ctggccgctg gcgtgttact tgtcatgttc cgcccgttcc gtgccggaga atatgttgac
    13141 ctcggcggcg tagccggtac tgtgctgagt gtgcagattt tctccaccac catgcgtact
    13201 gcagacggta aaattatcgt tattccgaac ggtaaaatta ttgccggaaa tattattaac
    13261 ttctcccgcg agccagttcg ccgtaacgaa tttattattg gcgtggcgta tgattccgat
    13321 atcgatcagg ttaagcagat cctgaccaat attatccagt ctgaagatcg cattttgaaa
    13381 gatcgcgaaa tgactgtgcg cctgaacgaa cttggtgcat cgtcgattaa tttcgtggtc
    13441 cgcgtctgga gcaacagcgg cgatctgcaa aacgtgtact gggatgtgct ggagcgtatt
    13501 aaacgtgaat ttgatgccgc cggtatcagc ttcccgtacc cgcaaatgga tgtgaacttt
    13561 aagcgggtga aagaagacaa agctgcgtaa tcaacgctga ggccagataa tactctctgg
    13621 cctctctctt attagttatt ctgattgcca attaatatta tcaatttccg ctaataacaa
    13681 tcccgcgata tagtctctgc atcagatact taattcggaa tatccaacgt gttttcttat
    13741 tactttcaag gtcttgctct tggggcagct atgattctgc cgctcggccc acaaaatgct
    13801 tttgtgatga atcagggcat tcgtcgtcag taccacatta tgattgcctt actttgcgct
    13861 atcagcgatt tggtcctgat ttgcgcgggg atttttggtg gtagcgcgtt attgatgcag
    13921 tcgccgtggt tgctggcgct ggtcacctgg ggcggcgtag tcttcttgct gtggtatggt
    13981 tttggcgctt ttaaaacagc gatgagtagt aatattgagt tagctagtgc cgaagtcctg
    14041 aagcaaggca gatggaaaat tatcgccacc atgttggcag tgacctggct gaatccgcat
    14101 gtttacctgg atacttttgt tgtactgggc agccttggcg ggcagcttga cgtcgaacca
    14161 aaacgttggt ttgcgctcgg gacaattagc gcctctttcc tgtggttctt tggtctggct
    14221 attctcgcag cctggctggc accgcgcctg cgcacggcaa aatcacagcg cattatcaat
    14281 ctggtagtgg gatgtgtcat gtggtttata gccttgcagc tggcgagaga cggtattgct
    14341 catgcacaag ccttgttcag ttaggcgcta tctgatggaa aaataaaaca gaggcgctaa
    14401 gcttgcctcc agaggtcctg aattttgggc aagtcaatta acacggagag actaacgtga
    14461 agttcaaagt tatcgccctg gcggcattaa tgggtattag cgggatggca gcgcaggcta
    14521 acgaattgcc ggatggaccg catattgtca cctccggtac ggcaagtgtg gatgcggtgc
    14581 cagacattgc cactcttgcg attgaagtta acgtggccgc gaaggatgcc gctacagcca
    14641 agaaacaggc agatgagcgc gtcgcacaat acatttcctt ccttgaactc aatcagatcg
    14701 cgaaaaaaga tatcagctca gcgaatttac gcacccagcc agattatgat tatcaggatg
    14761 gtaaaagtat cctgaaaggc taccgcgctg tgagaacggt ggaagtcacg ctccgtcagt
    14821 tagacaaact gaattccttg ctggatggcg cgctgaaggc gggtcttaac gaaattcgtt
    14881 ctgtgtcgct gggcgtggcg cagccggatg cctataaaga caaagcgcgt aaggcagcga
    14941 ttgataacgc gattcatcag gcgcaggaac tggcgaacgg ctttcatcgt aaactggggc
    15001 cggtatatag cgtgcgctac catgtttcca actatcagcc cagcccaatg gtgcggatga
    15061 tgaaagccga tgccgcgccg gtgtctgccc aggaaactta cgagcaggcc gctattcagt
    15121 ttgatgatca ggtcgatgtg gtcttccagt tagaacctgt ggatcaacaa cccgctaaaa
    15181 cacctgcagc acaataatac ttgtagcccg acgtattcgt cgggctaatg cctttacccg
    15241 atattgcctc ctttttctgt aattttctgt aaaaccatga cgtaatcacc tatctttgct
    15301 cgcggtttca tcaaggatag gctgactgaa acggttaagc ggacaaggat gcggggaggg
    15361 gggatggaca tttttatttc gaaaaagatg cgcaatttca ttttattagc gcaaaccaat
    15421 aatattgccc gggcggcaga aaaaatccat atgacggctt cgccatttgg taaaagcatt
    15481 gccgcgctgg aagaacaaat tggctatacg ctatttaccc gcaaagataa taatatcagc
    15541 ctcaataaag ccggtcagga actgtatcaa aaactgttcc cggtttatca acgactttct
    15601 gcgattgata atgaaatcca taactcaggg cgtcgttcac gagaaattgt gattggcata
    15661 gataatactt atccgacaat tattttcgat cagttgatta gtctcggaga taagtatgaa
    15721 ggcgtgaccg cccagccagt tgagttcagc gaaaacggtg ttatcgataa tctttttgac
    15781 cgtcagttag attttattat ctccccacag catgtatctg cacgagttca ggaactggaa
    15841 aacctgacta tcagtgaatt accaccgctg cgattgggct ttctcgtttc tcgccgttat
    15901 gaagagcgtc aagagcaaga gctgcttcag gagctgccct ggctacaaat gcgctttcaa
    15961 aaccgggcta acttcgaagc aatgattgat gcgaatatgc gaccttgtgg gatcaatccc
    16021 accattattt atcgtcccta cagttttatg gccaaaatca gcgccgtgga gcgcggacat
    16081 tttctgacgg tgatcccgca ttttgcctgg cgactggtga atccggcaac gctgaaatat
    16141 tttgatgcgc cgcacaggcc gatgtatatg caggaatatc tttattcaat cagaaatcat
    16201 cgttataccg ccacgatgct tcagcatatt gctgaagatc gtgacgggac aaaccattaa
    16261 cccagcatcg agccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    16321 tggtgaatat gtccgccggg cgcattttcc agatagcgat gcagataatc ccgatacata
    16381 ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga
    16441 tcggcgatcc cttgttcggt gatgatcact ttgacgctgt gttcgctgtg atcaacatgg
    16501 ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg agccataaag
    16561 atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg
    16621 accccggcta cgtgcgttga gttggcatgc ccgtaaatat caaactccag gccgacgttc
    16681 agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc ctgcggacgc
    16741 aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc
    16801 gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag taaatgcacc
    16861 accgattcct gtagcacttc cgaatacatc atgaacggag gaatttccgg gttttccccc
    16921 agacgcgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg cagaaattcc
    16981 ggcggaatac gcccatgcgc catttcctgc aataagaacg tgaccacgtt atcggcaatc
    17041 tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa gttggtttcc
    17101 acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac gcgatccatt
    17161 gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac aatatctgcc
    17221 agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt cttcgctcgc
    17281 agcagccagg tcggcgcatt accgatcccg ctggttaacc agactcgacc atccggtgcc
    17341 agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta attgaccatt
    17401 tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt gatcttttta
    17461 cgtaaaccgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc gtcagaaagt
    17521 acatcgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg atacggcttt
    17581 ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg tgggtagggc tttcggcgaa
    17641 cccgccgggg taaagccgct aaatgccacc atgtcgttat gctggataat ttctgccgct
    17701 tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt cgcagggatt
    17761 aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcattcct tcctggtaat
    17821 cttcgctgtc ataaaccgcg cgtcgcatcc cctgaatacg ttcaaattca tcggagttca
    17881 tggtgtgcgc ttcgcccagt acacgcagct cttctttgat aacagcaatg gctaactgcg
    17941 ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct tccacttcca
    18001 caacatggtt gaggatgccg acagccaacg cgcgctgggc ggtgattggc gaagcggtaa
    18061 aaatcagttc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga atgccgacca
    18121 ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta ctggcggcga
    18181 taatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta ccttccacca
    18241 tcgaaatgat cggtttcggg aatttttgga tcatgcgggt gatttgacgc aatggatcat
    18301 cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtgg cctgcggaga
    18361 agactttgga tccactcggc gcgcgcaaaa tgatacagcg aatttccggc cggttgagat
    18421 cgcttaatgc ctgcataaga tcatcaataa agactttact taaggcatta agtttgcggc
    18481 catagttaaa ctcaatgacc gccactttgt tgatagtgac aacgttaaca tactgataag
    18541 acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag ctgccgcagg
    18601 ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg gtaatagcga
    18661 tcgaaatctt cattcgcgaa caggtgattc agtacttctt cttcggtctg cttacgcagc
    18721 cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt tagcgcggtt
    18781 ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag tgcgctacaa
    18841 gtcagaaccc gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag ggcactctcg
    18901 tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt gttgataacg
    18961 atcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc gccgccaccg
    19021 gcaatttgca acgagataaa acagtccacc atgcgggcga cttctgtttc cgactgcccg
    19081 acgccaaccg tttcgacaat cactacgtca taacccgctg cttcgcataa cagcattaat
    19141 tcccgcgctc gctgactggc accgcccaga tgaccggagg atggtaccgg gcgaataaac
    19201 gccgcttcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat gctaccgcca
    19261 gtgaccgggc tgctgggatc gaccgcaata accgcgacct ttaatccctc tcgaatcaac
    19321 aacatgccaa aggcctcaag aaaggtactt ttccccgcgc cgggggtgcc ggtaacgccc
    19381 agtcgcaggg tgttaccgca gtacggcata atggcatcaa gcagctgcgt acttagtgcc
    19441 tgatgacgcg ggtgacggct ttccaccagc gtcatggcct gggcgagtgt ggcacgctca
    19501 ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat gatgctggct
    19561 tatcagattc agtacgtcgc gcacactgtc gagcataggt gtacctggac cataaatcgc
    19621 cgccacgccg cgctcttgca ggaaggcgta atcctgcggc ggaatgacgc cacccgcgac
    19681 cacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg ggatcagcgt
    19741 tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcgtttt ctacggccag
    19801 gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga aaccgagatc
    19861 ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgt ccatcctggc ccatcttagc
    19921 gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt gcgcaacaat
    19981 ggcatcgaac tcggaggccg atttctcaga ctgatgatag ctttgcgcaa tcacgccggt
    20041 aacacactgg cttggcacca gataacggtc gaaagcgact tccagcgcat cggaaatttc
    20101 acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt tttcgttatg
    20161 ctgtgcggcg tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat cacgggtggc
    20221 gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt cgatctcaag
    20281 tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga tgacacgctt
    20341 gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc gttttggcag
    20401 acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga taatagctct
    20461 ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg ccagtggatc
    20521 gacggtgcgg cagagttctg attcttcctg gatgatgatc tgggtgttgc gggcaatgcg
    20581 tgctgagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat gcagtgactg
    20641 agtaccgccc agcgtcgcag ccagtgcttc aatggtggtg cggataacgt tgttatacgg
    20701 atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg ccagtgattt
    20761 cggatcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg ccgcacgcaa
    20821 catggcgacg ttcataaaca gatccatgcc gataccgaag aagaacgaca ggcgaggagc
    20881 gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa tcccatcagc
    20941 gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcccccatgt ggtaaccgct
    21001 gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga tgtcggcgat
    21061 aatgcgcatt gacggttttg gtgggtaaat ataggtgttg cggcagaggt actctttgag
    21121 gatatcgttc tgaatggtgc cggtcagttt atcaggtgta acaccttgct cttctgcggc
    21181 gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg aaaccgacat
    21241 tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt cgatagcgac
    21301 gcccgctttg ccgacgtcgc ccgccacgcg cgggttatcg gagtcgtagc cacggtgggt
    21361 ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac ggcgataaaa
    21421 agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc acggttgggc
    21481 ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa gggtacctgt
    21541 cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga tcccttccgc
    21601 ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct tgttggcaag
    21661 ctgttgccac tcctgcacgt tagacattat ttgctccgta aaacgcaatc aaaatgaggg
    21721 tatttgcgcc tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa aatgcattca
    21781 gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa ggaatacaaa
    21841 aagaagtgag gacaacgcct gatatgtgct atcaggcatt atttgatgga ttaatcctga
    21901 cgaaggactt tgtgaccata atcgagtaac gcatcagtga ctttacgcat catgcggctt
    21961 tcaggagcaa agcggtgcca gtagagcatc cgtcgttgaa atagcccagg cgttaagtca
    22021 atcagttcac cgctggccag ctctttctcg atttgcaggt gcgggatcat acagcaggtg
    22081 gtgccctggc gagcaagttg tacgaacgct tctgaagaat taacgatatg gcagggcacg
    22141 ctgcctggag gcagatcgaa gttttgctgc aaaaaggcct ggtgcatatc gtcaagatgg
    22201 tcaaacgcga ccactggcgc tttcagtaat gccgaacgcg ttacgccgtt agggaaatat
    22261 ttttcggcaa agggttttga gctgacgaac agatagtcga gcgcaccaag tttatcgaca
    22321 agacaactcg gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc gccgcggcgc
    22381 agacgttcct gagtgcgggt ttcatcttct acctgcaagt tgaggcggat aggcgaatca
    22441 gccaacacag gagccagtgc aggaagcaac cacgtcgcca gactgtcggc gttgaccgcc
    22501 agtgaaagca gcagcggagt cgaaccggtt tgttcatcgc ccagccactc ttcttccagc
    22561 aactccacct ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg gcgcggcggt
    22621 acggtacgca ccaacagcgg ctgcccgaac atattttcca gttgcttaat gcgctgtgag
    22681 acggctgatt gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg ttcacgtatc
    22741 accgcatcca gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt tgtctgctcc
    22801 tggtaaatgc tttttcctgc actatgacac aattttgtgt caggttgcat ataccgaagc
    22861 cgtcggcggg taacatttgt attcgtggtt aaacgcgcag cttaaaagat cagggatttg
    22921 cgctaatgca tttcgctcag cgagttcgcg ccctggttgt gttgaatggc gtggcgttat
    22981 tgcctctgtt cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta tttgcggggc
    23041 gaaaggggat gcccgccatt gcgcgatatt ttatggatga gttaaccacg cggcttgcca
    23101 acggggtctg aatcgctttt tttgtatata atgcgtgtga aatttcatac cacaggcgaa
    23161 acgatcatga cgcaggatga attgaaaaaa gcagtaggat gggcggcact tcagtatgtt
    23221 cagcccggca ccattgttgg tgtaggtaca ggttccaccg ccgcacactt tattgacgcg
    23281 ctcggtacaa tgaaaggcca gattgaaggg gccgtttcca gttcagatgc ttccactgaa
    23341 aaactgaaaa gcctcggcat tcacgttttt gatctcaacg aagtcgacag ccttggcatc
    23401 tacgttgatg gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg cggcggcgcg
    23461 gcgctgaccc gtgaaaaaat cattgcttcg gttgcagaaa aatttatctg tattgcagac
    23521 gcttccaagc aggttgatat tctgggtaaa ttcccgctgc cagtagaagt tatcccgatg
    23581 gcacgtagtg cagtggcgcg tcagctggtg aaactgggcg gtcgtccgga ataccgtcag
    23641 ggcgtggtga ccgataatgg caacgtgatc ctcgacgtcc acggcatgga aatccttgac
    23701 ccgatagcga tggaaaacgc cataaatgcg attcctggcg tggtgactgt tggcttgttt
    23761 gctaaccgtg gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa aaccattgtg
    23821 aaatgatctg acgggggaac ctcccccgtt aaaaaaattc tcttcattaa atttggtgac
    23881 atgtgtcacg cttttaccag gcaattgtcg attgctctaa ataaatcctc taaaccagca
    23941 tattcatcca agaattacct ttgcgtgata tttcctcaac atcgcgacgc aaacgttcat
    24001 attgccgcaa tattattttt tgatatgttg aaaggcggat gcaaatccgc acacaacatt
    24061 tcaaaagaca ggattgggta aatggcaaag gtatcgctgg agaaagacaa gattaagttt
    24121 ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc
    24181 aacatcgaat ttcacaaagg cgcgctggat gatgaacaat taaaagaatc catccgcgat
    24241 gcccacttca tcggcctgcg atcccgtacc catctgactg aagacgtgat caacgccgca
    24301 gaaaaactgg tcgctattgg ctgtttctgt atcggaacaa accaggttga tctggatgcg
    24361 gcggcaaagc gcgggatccc ggtatttaac gcaccgttct caaatacgcg ctctgttgcg
    24421 gagctggtga ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa
    24481 gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag
    24541 ctgggtatca tcggctacgg tcatattggt acgcaattgg gcattctggc tgaatcgctg
    24601 ggaatgtatg tttactttta tgatattgaa aacaaactgc cgctgggcaa cgccactcag
    24661 gtacagcatc tttctgacct gctgaatatg agcgatgtgg tgagtctgca tgtaccagag
    24721 aatccgtcca ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa gcccggctcg
    24781 ctgctgatta atgcctcgcg cggtactgtg gtggatattc cggcgctgtg tgatgcgctg
    24841 gcgagcaaac atctggcggg ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat
    24901 agcgatccat ttacctctcc actgtgtgaa ttcgacaacg tccttctgac gccacacatt
    24961 ggcggttcga ctcaggaagc gcaggagaat atcggcctgg aagttgcggg taaattgatc
    25021 aagtattctg acaatggctc aacgctctct gcggtgaact tcccggaagt ctcgctgcca
    25081 ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt gctaactgcg
    25141 ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg cgcaatatct acaaacttcc
    25201 gcccagatgg gttatgtagt tattgatatt gaagctgacg aagacgttgc cgaaaaagcg
    25261 ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc gtctgctgta ctaattcccc
    25321 ttctctgaaa attaacgggc aggtcactga cttgcccgtt tttttatccc ttctccacac
    25381 cgttgccgcg ttgcgcattc tactttgcga gtctgctcca gaaaataaat caaagtcaga
    25441 tcaatgcgtt gtgtttattt aatggggatt tacgatggcg ggacagcatg gggctgtaac
    25501 ggtaaagccc tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg acttgtcatc
    25561 atactgattg tactgttact gataagtttc ggagcttatt aacagtcagt ctcaggggag
    25621 gagtcatcct cccttaccct tactcactaa attaggtcaa agaatcaacg atgtcaatca
    25681 gggcgatgcg gggtgcatcg ccctgaaccg aactttttga tagccacttc atcgcccacg
    25741 tttactgccg taatagatcc tttttcccaa aaattatctc tgttttgcat ggggtttttc
    25801 gagcacgctc gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat ttaaagagag
    25861 cttacgatag ctgggcagca tggggctgta acggtaaagc cctcaccgaa gcgagggctt
    25921 gaaggagaag ggttatgatg cgacttgcca tcatactgat tgtactgtta ctcataagtt
    25981 tcccagctta ttaacagtca gtcacagggg aggagcaatc ctcccttacc cttactcact
    26041 aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc gcccttacca
    26101 ctcccagact ttcgacggtg taaccaccgc aggaagaggg atatcccact cttcaacggg
    26161 gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcgttt tatagtgctg
    26221 ccagttttgt aaggtccgat cataaaaacc accgcccatt cccaggcgct gaccgtactc
    26281 atcaaaggcg accagcggtg tgatcagcac gtctaatcgg gaaaggggta gcacgtcacg
    26341 cacatccagt tttggctcat ggatcttcaa cctgttcatc accagttcgc tttgcggatg
    26401 gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa gatatacgcg
    26461 cttaccggcg cgccagagtt gttctattag cggctgggtg tcgagttcac catcaaaaga
    26521 gaggaataca gcgaccgtat gcgccatcac caccggggga taagtcatca tccgggtagc
    26581 ggcttgttga cccatttcct gctgttgttc cggcgttaac gcacgacgac gttgccgaat
    26641 cattttgcgg atttcttgtc gggataatgt cagtgggagt tctggtagtt gcgtcatggc
    26701 tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc cttgaaccct
    26761 tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacggacc gagcatgctc
    26821 accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg actggcccgc
    26881 ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta aaccgaaaag
    26941 tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag taacgcttgt
    27001 tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta gtcacgagtc
    27061 ttcgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa gaccaactgt
    27121 tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt cagatcgtcc
    27181 gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag tgaacggcca
    27241 aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt gactgcgctg
    27301 ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc tgatgagaag
    27361 agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg aatattcctc
    27421 cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca acgaaatgaa
    27481 ccagtatctg aaccaacaag ggacgggtct gacccctgct gagatgcatg gtttaatcag
    27541 tgggatgata tgtggcggta acgatgacag ctcatggcta ccgctacttc acgacctgac
    27601 gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa tgcactctgc
    27661 caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctgc ctgatggcga
    27721 tgatgtcagc gttttcgatc gggctgatgc attggcaggt tgggtcaatc acttcctgct
    27781 tggtcttggc gttacgcaac cgaagctgga taaagtgacc ggcgaaaccg gtgaagctat
    27841 cgacgatctg cgtaacattg cgcaactggg ttacgacgaa gacgaagatc aggaagagct
    27901 tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt tatgccacga
    27961 cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc tacactaaaa
    28021 acaaaaaacg taaggagagt gttatgagtg agatatcccg gcaagagttt cagcgtcgcc
    28081 gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt gctgcaccag
    28141 aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac ttctggtact
    28201 tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat gacactcata
    28261 accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg tttggccgtc
    28321 gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg gcattcagcg
    28381 aaatcaatca gcaactttat caactactta acggcctgga tgtggtttac catgcccagg
    28441 gcgaatatgc atatgctgat gaaatcgtga acagtgcgct ggaaaaactg cgtaaaggtt
    28501 cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt gttcatgaaa
    28561 tgcgcctgtt caaatcgcca gaagagattg ccgtactccg ccgcgcggga gaaatcaccg
    28621 ccatggcaca tacccgggcg atggaaaaat gccgtccggg aatgttcgag taccatctgg
    28681 aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg ctatccgtcc tataacacca
    28741 ttgtcggcag cggtgaaaac ggctgcattc tgcactacac cgaaaacgag tgtgaaatgc
    28801 gcgacggcga cctggtgttg attgacgcgg gttgcgaata caaaggttac gctggcgata
    28861 ttacccgcac cttcccggtc aacggcaaat tcactcaggc ccagcgtgaa atctacgaca
    28921 ttgtgctgga gtctctcgaa accagcttgc gcctgtatcg tccgggaact tccattctgg
    28981 aagtcactgg tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc ggcattctga
    29041 aaggtgatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt atgcatggcc
    29101 ttagccactg gttagggcta gatgtccatg acgttggcgt ttatggtcag gatcgctcgc
    29161 gcattctgga accggggatg gtactgaccg tagagcctgg tctgtatatc gcgccggatg
    29221 cggatgtgcc agaacaatat cgcggtatcg gcattcgtat tgaagacgac attgtgatta
    29281 ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa gaaatcgaag
    29341 cgttgatggc agctgcgaga aagcaatgag cgtaatcatc gtcggtggcg gcatggcggg
    29401 cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgctgccgg tacatttgat
    29461 tgaagcgacc gcgccagagt cacatgctca tccgggcttt gatggacgag ctattgcgct
    29521 ggctgcgggg acctgtcagc aactggcgcg catcggcgtc tggcaatctc tggcggatta
    29581 cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt cacgctggat ttgtcaccct
    29641 cgccgcagaa gattaccaac tggcggcgct gggacaggtt gtcgaattgc acaatgtcgg
    29701 gcaacggctg tttgcattgc tgcgtaaagc acctggcgta acgctgcatt gccctgatcg
    29761 cgtggctaac gttgcccgta ctcagagtca cgttgaagtg acgctggaga gtggcgagac
    29821 gctgacgggc cgcgtgctgg tagcagctga tggcacccat tcagcgttag ccaccgcgtg
    29881 cggcgttgac tggcagcagg agccttacga acaactggcc gtgattgcca acgttgcgac
    29941 ttccgttgcg cacgaaggcc gcgcttttga acgcttcacg caacatggcc cgctggcgat
    30001 gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg aacggcgcga
    30061 agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa ctccagtcgg cctttggctg
    30121 gcgacttgga aaaattaccc acgccggtaa acgcagtgct tatccgctgg cgttaacccg
    30181 cgccgccaga gctattaccc atcgtaccgt gctggtgggc aatgcggcgc aaactctgca
    30241 cccgattgcc gggcaagggt ttaacctcgg tatgcgagat gtcatgagcc ttgcggaaac
    30301 cctgactcag gcgcatgagc gcggagaaga catcggtgat tacggcatat tgtgccgcta
    30361 tcagcagcgt cgacagagtg atcgcgaagc aaccatcggc gtcacggaca gcctggtaca
    30421 tctttttgcc aaccgttggg caccgctggt tgtcgggcgc aacatcggac tgatgacgat
    30481 ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt gggtggcgcg
    30541 ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt gttggtggcg
    30601 gcatggtggg gctggcggtt gcctgcggct tacaggggag cggcttacgc gttgccgtac
    30661 tggagcagcg cgtaccggaa cctctggcgg cggatgcacc accacaactg cgcgtttcgg
    30721 ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag gacattctct
    30781 ctcgtagggc cagttgttat cacggtatgg aagtgtggga caaagacagt tttggtcaca
    30841 tttcgtttga cgatcaaagc atgggctata gccatcttgg gcatatcgtt gaaaactcag
    30901 tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc agatatcact ctgttggccc
    30961 ccgcagaatt acagcaggtc gcctggggag aaaatgaaac tttcctgacg ctgaaagatg
    31021 gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg tgctaattcc tggttgcgca
    31081 acaaagcaga tattccgctg actttctggg attatcagca tcacgcgctg gtagcgacca
    31141 ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt tttccatggc gaaggcattc
    31201 tggccttttt accgcttagc gatccgcatc tttgctcaat tgtctggtca ctgtcgccag
    31261 aggaagcgca gcggatgcag caggcaagtg aagatgaatt taatcgcgcg ttaaatatcg
    31321 cttttgataa tcgcctgggc ttatgcaagg ttgagagcgc gcgtcaggtg ttcccactga
    31381 cggggcgtta tgcgcgccag tttgccgcgc accgtctggt gctggtgggc gacgccgcgc
    31441 ataccattca cccgctggcg gggcagggag taaatctcgg ctttatggat gctgcagagc
    31501 tgattgctga actgaaacgg ttgcatcgtc aggggaaaga catcgggcag tacatttacc
    31561 tgcgccggta tgagcgtagc cgcaagcaca gtgcggcgct gatgctggct ggtatgcagg
    31621 gattccgcga tctgttttcc ggtaccaatc cggcgaaaaa actgctgcgt gatattggtt
    31681 tgaaactggc cgacacgctt cctggcgtta aaccacaact tatccgccag gcaatgggat
    31741 taaacgattt gcctgaatgg ctgcgttaaa aatttctctt ctgctgttta tttgataccc
    31801 atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga aataaactaa
    31861 tttcacctcc gttttcgcat tatattttct aatgtcacga atttttgatt tagtgttttt
    31921 tgacattttt tcagctctta atattgcctt attcaaatta
//
LOCUS       DAZFRJ010000036        31912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000036 DAZFRJ010000000
VERSION     DAZFRJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.036"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(552..641)
                     /locus_tag="ACHKUN_001505"
     tRNA            complement(552..641)
                     /locus_tag="ACHKUN_001505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(605..607),aa:Ser,seq:cga)
     gene            735..1532
                     /gene="mtfA"
                     /locus_tag="ACHKUN_001506"
     CDS             735..1532
                     /gene="mtfA"
                     /locus_tag="ACHKUN_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO6659795.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1633..1708
                     /locus_tag="ACHKUN_001507"
     tRNA            1633..1708
                     /locus_tag="ACHKUN_001507"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1666..1668,aa:Asn,seq:gtt)
     gene            1870..2785
                     /locus_tag="ACHKUN_001508"
                     /pseudo
     CDS             1870..2785
                     /locus_tag="ACHKUN_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2979..4283)
                     /gene="ybtS"
                     /locus_tag="ACHKUN_001509"
     CDS             complement(2979..4283)
                     /gene="ybtS"
                     /locus_tag="ACHKUN_001509"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO6659796.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4311..5591)
                     /gene="ybtX"
                     /locus_tag="ACHKUN_001510"
     CDS             complement(4311..5591)
                     /gene="ybtX"
                     /locus_tag="ACHKUN_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO6659797.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5584..7386)
                     /gene="ybtQ"
                     /locus_tag="ACHKUN_001511"
     CDS             complement(5584..7386)
                     /gene="ybtQ"
                     /locus_tag="ACHKUN_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO6659798.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(7373..9085)
                     /gene="ybtP"
                     /locus_tag="ACHKUN_001512"
     CDS             complement(7373..9085)
                     /gene="ybtP"
                     /locus_tag="ACHKUN_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO6659799.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            9342..10301
                     /gene="ybtA"
                     /locus_tag="ACHKUN_001513"
     CDS             9342..10301
                     /gene="ybtA"
                     /locus_tag="ACHKUN_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO6659800.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEQREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            10492..16599
                     /gene="irp2"
                     /locus_tag="ACHKUN_001514"
     CDS             10492..16599
                     /gene="irp2"
                     /locus_tag="ACHKUN_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO6659801.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            16687..26169
                     /gene="irp1"
                     /locus_tag="ACHKUN_001515"
                     /pseudo
     CDS             16687..26169
                     /gene="irp1"
                     /locus_tag="ACHKUN_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
     gene            26166..27266
                     /gene="ybtU"
                     /locus_tag="ACHKUN_001516"
     CDS             26166..27266
                     /gene="ybtU"
                     /locus_tag="ACHKUN_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO6659802.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27263..28066
                     /gene="ybtT"
                     /locus_tag="ACHKUN_001517"
     CDS             27263..28066
                     /gene="ybtT"
                     /locus_tag="ACHKUN_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO6659803.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            28070..29635
                     /gene="ybtE"
                     /locus_tag="ACHKUN_001518"
     CDS             28070..29635
                     /gene="ybtE"
                     /locus_tag="ACHKUN_001518"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO6659804.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYHAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            29766..31787
                     /gene="fyuA"
                     /locus_tag="ACHKUN_001519"
     CDS             29766..31787
                     /gene="fyuA"
                     /locus_tag="ACHKUN_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO6659805.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         6885 a         9571 c         8819 g         6637 t
ORIGIN      
        1 tttattatgt ctgtagaaaa aaatgcgaaa aaggcgaaca ataaatacac aactcagcca
       61 tacagactgg cagcgcaaat gtttccagga gcaagtcatc tgtcatggta aagctaacca
      121 gtaatatagg ttaattttgg tgtttaaacc ttttttactt gttagtatga tacaaaggct
      181 ttcaaaaaag ctgggaaatt caacaactct tggaaattat ggactttgtc ccgcggcttt
      241 aatctgacgg ccgcgttcct tttttgactc aattattgac cccttctcta cgcaacttca
      301 gttcccgcca gcaactttgc ggcagtttca taggatcaat gtctaaaaga ataatggtga
      361 ccgataagaa aacgactgaa taactgtaga ttttcgctcg aaaccttcct gtcagctcca
      421 tagcgaatca agtgctgaat gtcacagtat cgaacagaaa acagtgacga tctaaccctt
      481 caagaatatt ctacgattgt tctgtttagg aaaagcaagg cgggaagtca ggagataagt
      541 cattgataaa gtggcggaga gagggggatt tgaacccccg gtagagttgc ccctactccg
      601 gttttcgaga ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga
      661 tgccaggaaa tttggcattt taacagtccc tgtccgtgca attttgttca agtgacgagt
      721 ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg
      781 cccttccctg gcaggaagca ctatcgatcc cccttttaac gggtctgacg gaacaggaac
      841 aaagcaaatt agtcactctt gccgaacgtt ttttacagca aaagcggctt gttcctttac
      901 agggctttga actggattca ttaagaagct gccggatagc acttctattt tgcctacccg
      961 ttctggagtt aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc
     1021 catttgtggt cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg
     1081 ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag
     1141 attcttttga tgcatctggt tttaacctga ttattcatga agtcgctcat aagctggaca
     1201 cccgtaacgg cgatcgcgcc agcggagttc cctttatttc gttgcgtgag gttgctggct
     1261 gggaacacga tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttagttggtg
     1321 agaatgcagc gagcattgat gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac
     1381 tttctgaata tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc
     1441 aacgtttctg tcaattttat caacaagatc ctttgcagag actgcatcac gctaatgata
     1501 cagactcgtt ttcggcgacg aatgttcatt aattaacaac tttgcagatt aattaaccaa
     1561 ttgaaatggc ttatgaaatt tagtgttgac agacaaggta ccgctaagta atatgcgctc
     1621 cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
     1681 actggttcga gtccagtcag aggagccaat tttctgtttt catgcatcct tgcaaatctt
     1741 tatctgatgt tgattcaaca ggttagtgtg aaaactcttc tcggtgcatt ttgattttac
     1801 cctatgcatc gggaaaaatt ggtggtcaaa tctgggatca ggttagttcg ataatggagt
     1861 gacccccata tgttccttac cgacgcaaaa atccgcaccc tcaagccttc tgataaaccc
     1921 tttaaagtct ccgattctca cggtctgtat ctgctggtca agccgggtgg ctcccgccac
     1981 tggtatctca aagctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     2041 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     2101 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     2161 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     2221 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     2281 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     2341 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     2401 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     2461 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     2521 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     2581 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     2641 taccgtgcgc atggcttata ttcataaggc agagcaccta gaagcccgca aagcgatgat
     2701 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcgccgc cttatacaat
     2761 tggtaaaaat aagtttatcc catagtatac taacgaatcc ctcactctgg caaattacca
     2821 ccacctccac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
     2881 attttatccc aaaagaagac aatagagtca cagaggaatc aaagagcgac tcaattgtct
     2941 ttggacagtc tggttgtgag gttagaaaac agttactcct acaccattaa atagggcgca
     3001 atgctcgcta atttctcccg ggtttctgtc agttcgcgtt ccggcgtaga ttgcgcgatg
     3061 attcccgccc ccgcctgtat ccagcagcgc tggctatcct gaaatacgga acgcagaact
     3121 agcgccgcat caaagcgcgt atcgtccagc agcaggattg cgccggaata aagctctcgc
     3181 ggcgtcttct caatttgcat aatcgcgttc agagcagcat ttttagggat acctgaggcg
     3241 gtaatcgacg gaaacagcac ggtaaacgca tcccaggcat ccttgttttc cgaaagctga
     3301 ccgctcacgc cagaccccag atgctgaacg ctgccgcgct ggcgaaccga cattaaatct
     3361 tcaaccacca cgctgcccgg ctggcaaaca gcctccagtt cagcaatagc ttctttgaca
     3421 gaaaggatat gctcaagcac ctctttactg tcgtgcagca gttctgcctc tttcgcctta
     3481 ttatgctccg ggtttcccat gcgatcgcgg gtgcccgcaa gcggttcagt gaccacttta
     3541 ttgcccatca ctgacatcac cagttccggg ctaaagccca gcgcttcgcg tccttcctga
     3601 cggaacataa acgagcgcac aggcgtgttt gcctgccgcc cgtataacag cgtggcgggc
     3661 atatcaatcc gcgatggcag gggaatggcg cgcgagacaa tcactttgac atactcgcca
     3721 cggcggatct ccgcaacggc gcgcgcaacc tgttgtttat atgcctcacc attgagggcg
     3781 gtatccaccg ccagtggtgc gttctgcgtc gttgtaccgg cctcttttac ccactcgcac
     3841 aaacgtcggc acccgtcggc ggagtccgca taaacggtga catttccctt ttcaaaaata
     3901 agttcttcac ggggaacggt taacgtcagc agcggccact ccccggcgtt aaaggcaatc
     3961 ccccgcgcat gcgccgcaaa gttgaaccca acctgtccat atacccgacg tcctttcacc
     4021 tgaggatgcg ccataaaacg acgcgcgcag tcggcaatgg aatccaccgc ccatttttgc
     4081 tccccggcat catcaataaa tgtcgcctgc gttccgtcgg cattaatgtg cagccgtgcc
     4141 tggcaccctt tgcctaaata ccaacagggt tgacgctcat agacataaca ctcttcttct
     4201 gcgaactggc gtaaaacgcc agaaatcgtc ggtagccatt gttcctctgg taacgccaga
     4261 tgtagaaatt cactgatttt catcccttac ctctctgtgt tattcgcgga tcaatcaatt
     4321 gcgcttgtca tcagttctct tgccgaatgc agccggatat aagcaaccca cgccgccagc
     4381 aacgtgaatg ccccggcaaa caggaagcag gccgcatagc caaaatgttg agcaacaacg
     4441 ccaccgataa cgcctgccag aatggcgatt gccgcatcag tacattgaaa gagggtgaag
     4501 tcgacaccgg cctgcaaagg cgatgaaagc gacatcagcg tggcgtaaag acagaccagc
     4561 gcgcaggcca gcgaaatgga ctggacaatg accagacact ggagaatctg cagcagcaga
     4621 tgacccggcg ccatcatgag cgtcatcacc accgccagca gcgcgatccc ctggacgcca
     4681 taagccgtca acagcgctct gccgggtgag gtgtatttca tcagcaatcc gccagccagc
     4741 gttcctgcta tgcccgctgc aatattgccg ccgctgaaca acgcgcccaa tgcggacatg
     4801 ctcaacccat gatccaacaa cagcggtgcc agaagaggca gcacaaaccg catgcctgaa
     4861 ttcagcatca ataccagcag taagcccagg cgcgcctgct tcctccttag cgcataacct
     4921 aacgccggaa catgcaggat agtcgctgtt cgcgtcggct ccgtaatgcg ccacagcggg
     4981 agtgacagcg ccataatcag caccgccatc atcagcatgg cgacaggcca gccggatgcc
     5041 gccgacagca tcatgaacac cccgccgccg cacatcattc ccagatagct gccgccgacc
     5101 tgcacgctgt ttccccagcc gtaacccgtg cgggtcagtt gatcgacaca aaagccgtcg
     5161 ctggcgatat caatggtgga ggcgaccgtg cctgccacca taaacagaac aaataacgcg
     5221 acgatttgcg tatcggggaa tcccccttct cgcctaaacc agccaatcgc tgcggcaata
     5281 gtcaggatcg ccgctagcgc gacctgacca cgcaggatta acatgcggga acggcgttcc
     5341 tggctaccgg gcggaagccg ccagcgctcg atccacggcg cccaaataaa cttcagcgcc
     5401 cagggcagca taaacagggt tgtcgcaccg gcaagcgcca gcgaaccgcc agcagcgcgc
     5461 accagcgcgg gtaaggactg catagacagc gccgatacca gactctgcgt cacatagacg
     5521 ccggcaatag caaaaatcac ccgcccggtc gtcaacgtca gcggtttcac attcgactga
     5581 acatcactca tgcacccact ctccagacgc ggaaaccccg tcgtcgcgcc acacgcgcgc
     5641 ggccatttgc gcctgccaca gcgcctgata acgcccatga tgtgagagca attgcgcatg
     5701 agtaccctgc tcaaccacct gtccctcttc catcaccaga atgtttccgg ccccggcgat
     5761 ggtggataaa cggtgagcga tgataatcac cgtccggttg taaaccaggt tatcgatcgc
     5821 tttttgcacc gccagctcgc tttcaatatc cagcgcggca gtcggttcat cgagaatgac
     5881 gactggcgcg tttttcaata acgctctggc aatggaaatc cgctggcgct cgccgcccga
     5941 tagctggccg cccatctcac ccattggcgt cagccagcct tgcggaagac gggaaataaa
     6001 ctcaaggcac tgcgccgcgc gggcggcctc ttctacctcc tgccgcgtcg cctgtgggca
     6061 cgcgatacgg atattcgcca gcagcgtgtc atcaaacagc cagacatcct ggaaaacaac
     6121 agaaatcagg ctattgagct gttccggcgt caggcggcga atatcgacgc cgccaataga
     6181 aatctgccct tgctgcggat cggcatagcg cattaacagt ttggtgacgg tagttttgcc
     6241 tgcgccggag gcacccacca gcgcgctcat actggctgcc gggaacgtca aagaaacatg
     6301 attaagcgcg tggccgtcgc cttcttcgta gcgatagctg acgttgtcaa agcggatatc
     6361 gtaacgttcc ggcatctcgc tttgctctgc gacgggaagc ggtgctatcg ccataaactg
     6421 ctcaatccgt tgcagggcgc tggcgatcag ttccacaacc gaggtgtagc tgataaacat
     6481 cgccatcggt tctgcgaagc gcataatcat cgcgacggcg gcaatcaaaa aggcgaggtt
     6541 cagagtgccc gtcactaccc agacgatccc ggataacacc accacctgta ggcccaactc
     6601 cacgacgctg gcgatcagca tcgtcgcgcc agcgccctga cggtgagtgc gggtctgtaa
     6661 gttttccagc gcattgaaat gcgccagcag cgcccggctt ttatcggcat cgctgccgca
     6721 ggtgcgcaat accatcatcc cctgagcaaa ttcaacgata tcgccgctca gacgctggtg
     6781 cgcttccccc agcgtctgca tttgtcgccg catcgccggg cggcgccagt aataaaacgg
     6841 caccagcaga gggaagatca gcaacatcac cagccccagc cgccagtcta tccacaatgt
     6901 cgccagcgac gccgtcagcg gcgtgacaat ggtgagcagc aaaatattgg ctatcgcaat
     6961 aacataattg aggttttcat ccacgctgcc cagcagcaag gcgttcattt cacccgccct
     7021 gccgcgctgg agcttctcca gcggcacgcg gcgtaactgc tcgccaagtc gcaggcgcaa
     7081 ctcatgggta gcctgcgcca gatgaccacg gtattcaaag cccaggccat accagcgtag
     7141 caccagcgtc acaattgcgg cgacgctgaa ggccatagcc cagttaagaa gttgcggcgc
     7201 gtctccccgt aacagcgcat caatgatcgg ataaagacag gcaaaggcga tcccctgcat
     7261 gaatgccgcc agcaacagcg ccagcatact gcgtcgcagc atcctggcct gactgccaac
     7321 gctggagata agctggcgcc agatgacgcg ccaggccagg ttatccgcag gattattgtc
     7381 tttcattttc caccacctct tcctgtgttc cacccggcac ccaatgctgc gcctgctgat
     7441 agtgttgcca gagccgttga tacaggccgc cctgcgccaa cagttgcgta tggttcccca
     7501 tttccctgag ctgtccgtcg gaaaacagca atatcacatc ggcctgagtc accattgaga
     7561 gacgatgcgc gaccatgatg accgtccggc cacgcatggc cgccgcgagc gccttgataa
     7621 gcgccgcttc gttttccggg tcagcaaacg ccgtcgcctc atcgagcacc aggatggggc
     7681 gatcctgcaa aagcgcccgg gcgatagtaa tgcgctgccg ctggccgccg gagagaaata
     7741 ccccacgttc cccgactcga gtgttgtaac cttctggcag agcgctaata aaatcatggg
     7801 cctgcgccac tctggccgcc gctattaccg cctccagcgg cgtatccggc gcgcccagac
     7861 gaatgttatt ggctatcgtg tcgacaaaaa ggaagttgtc ctgaaacaca aacgagagtt
     7921 gcttcatcag ggtgtccgtc tgcatatcac gcagatccac gccgccaata cggatatggc
     7981 ctttgtccgg gtcggcgtaa cgcagcagca agcgcgccac ggtgcttttt ccggcgccgc
     8041 ttggcccgac cagcgccaca atttgcccgg caggcacatg aaagctcacc tcctgcaacg
     8101 cggcgccagt acgcgcttgc ggataatgaa agctcacctg ctcaaaggta atgctcgcct
     8161 cttgcggctg ctgctcagac tgcggcagcg ataactccgg catcgcgaga acctgataaa
     8221 tacgctgaat gcttaaacgc gtttgcgcga ccaggttatt gagcatcatc attggcatta
     8281 cggcttcggc cattccgctg cccagcagta aaacggccac ccacgcgata aaatcgaaac
     8341 tgccatagtg caacaggccg tatcccgacc agatcaggac aaacagggtc gggagaggat
     8401 tcagaatcga gaaggaaaaa cgcgctgaaa aaccggcttt gcgataccag gtttggagca
     8461 catcgaccca ctcttcaagg gcgcgttgat agcgcaaaaa actggtgctg ccgctatcaa
     8521 acgttctcac cacgggcatg gcctgcacga actcaataac cgccgcgcta acctgctcgc
     8581 gcgccttatg ataacgctga gccatatctt ccgaacgctg gcgggcgagc acgagaacaa
     8641 ccgatccaaa cgccagtacc cccagcgtcg cgattgccag ccgccagtcc agccagaaca
     8701 agataacgat tgtcgccagc ggcatgatga tcgcgcgggc atagagcggc gtgctgtcgg
     8761 ccacaaaaat atgtaacgac ttcacgtcat ccagcatcac tttcgccact gacccgctgc
     8821 ccatttgctg taacacgcca ggcggaagct gcaatgcttt acgggctaac ctgctgcgca
     8881 gaatgttctc cagatgaaaa gccgcgtaat gagaggtgtt gaacgcctgt aagcgcagaa
     8941 caaaagttaa gaccacgcag ccgatcacgc cacccagcgg ccaggccgga atagcgtcag
     9001 gcgtcgcgcg aatgtcacgc aggctccaca ccagaaaaag gagcgccccc aggctggtta
     9061 atgccgccag ccccgccagc gccatagcta aaataacctt cctgcgaacc ggtgtaatca
     9121 cctgccagag aggaaatcgg gacagcgatt cagtattgga tgattgagat gacatgggag
     9181 taactgaatt tcctgatgca tttttacgga cgccaaaaac gacaattcag ggtggcgttc
     9241 tgagaattaa tgtgaataat aaccattatc aataactcat ctaccccatt cggttctatt
     9301 tatacccgca ttggtctaag ccacagggag ataaccaggt catgacggag tcaccgcaaa
     9361 cgcaatctga aatctctatt caccagttgg tggtcgggaa acctgcaaac gatggcaata
     9421 ttcctgctca gtgtgaactg ctgcgctgtt cgttgcagga aggtatggat attttgctct
     9481 ggaggggcca ttttgcgcgg cctgagacgt tgcagctaca cgacgatctg ggcaggatta
     9541 acttctcgtg catcctggaa ggcacatcgc gctttgcgat tcagggactc cggcgccaca
     9601 ccgactggga gcttgcgcgc aatcgccatt acattaccca tacgccagac tgtcgcggca
     9661 gcgcctccta ttgtggccga ttcgagagca ttaccctctc cttcagccct gaaaccctcg
     9721 cgctgtgggt gccggatatt agcgcggtca ttaagaataa gatcgactcc cactgctgtt
     9781 gccagcagca gcgttgtaat gctgaaaccc acttaacggc tcaggccttg cgtcacgcgc
     9841 tgatgcgcat gcacgacggc ttcaatcatg aacaaaagcc gtcgacgcta tggttgcagg
     9901 ggcaaagtct ggtcatgctc agtctggtgc tggatgaaca gcgcgaagac gccagttgcc
     9961 tctcctgcca cttcaacccg atggaacgcc agaaactgct gcgggcgaag gatctgctgc
    10021 tggctgatct gacgcaagcg ccgggcgtcg cggagctggc cagagagtcc ggtctgagcg
    10081 ttctcaaaat taagcgaggc ttccgcgttc tgttcaacaa cagcgtctat ggcctgtttc
    10141 aggctgaacg aatgcaggaa gccagaagac gcctggccaa cggcaatacc tccgtcatga
    10201 cggtggcggc cgatctgggc tatgccaacg ccagccattt ctctgccgcc ttccagaaac
    10261 agtttggcgt aactccttcg acctttaaac gcgggatgta accggggtcg cgccccccta
    10321 aactcctccc tgacagaggc ccggataccc catccgggcc tctgttcttt ccgcctgtta
    10381 ccaacccgaa acgggcataa ataaacccgt ttcgggtagc atccaattgt taataattat
    10441 tattctcata tgagtaatgc ttttcggtaa gacgtgccat caggaggaag aatgatttct
    10501 ggcgcaccat ctcaggattc gctgttaccg gacaaccgcc acgcggctga ttaccaacaa
    10561 ttacgcgagc ggctcataca ggaactgaat ttaacgccgc agcagttaca tgaagagagc
    10621 aacctgatcc aggccggtct ggattccata agattgatga gatggttaca ctggtttcgt
    10681 aaaaatggct accgccttac ccttcgcgag ctgtatgccg cccccacgct ggcggcatgg
    10741 aaccagttaa tgctcagccg gtcgccggag aacgcggaag aagaaacgcc gcccgacgaa
    10801 tcatcctggc cgaacatgac cgaaagtacc cccttcccat tgacgccagt acagcacgcc
    10861 tacctgacgg gccgcatgcc ggggcagacg cttggcggcg tgggttgcca cctgtatcag
    10921 gagtttgaag gccattgtct gacggcgtcg cagctggagc aggccatcac gaccttgctg
    10981 caacgccacc caatgctgca tatcgccttt cgccccgacg ggcagcaggt ctggctaccg
    11041 caaccttact ggaacggcgt caccgttcat gatttacgcc ataacgacgc tgaaagccgc
    11101 caggcctatc tggacgcact gcgccagcgc ctgagccacc gtcttttacg cgtggaaatc
    11161 ggcgaaacgt ttgattttca gctgacgctc ttgccggaca atcgccaccg cctccatgtc
    11221 aatattgacc tgctgattat ggatgcctcc agctttacgc ttttcttcga tgagcttaac
    11281 gccctgctgg ccggagaatc gctgccggct atcgacaccc gctatgattt ccgctcgtat
    11341 ttgctgcacc agcagaagat caatcaacca ctgagagacg tcgcgcgcgc ttactggctg
    11401 gcgaaagcat cgacgcttcc ccccgcgccc gtcttgccgc tggcctgcga acccgccacg
    11461 ctacgtgaag tccgtaatac ccgacgccgc atgattgtcc cggcaacacg ctggcacgcc
    11521 tttagcaacc gggccggcga gtatggcgtg acgccgacaa tggcactggc gacctgtttt
    11581 tctgccgtgc tggctcgctg gggcggcctg acgcgtctgc tgcttaacat caccttattc
    11641 gaccgccagc cgctgcaacc ggcggttggc gcgatgcttg ccgacttcac caatattctt
    11701 ctgctggata ccgcctgcga tggcgatacc gtcagcaacc tggcgcgtaa aaaccagctc
    11761 acgtttacgg aggactggga gcatcgccac tggtccggcg tcgaattact ccgtgaactc
    11821 aaacgccagc agcgctaccc ccacggcgcc ccggtggtat ttaccagcaa tctggggcgt
    11881 tccctctaca gcagccgcgc agaatcgccg ttgggcgagc cggaatgggg catctcgcaa
    11941 acgccgcagg tctggataga tcatctggcg ttcgagcatc acggcgaggt ctggctacaa
    12001 tgggacagca acgacgcgct gttccctccg gcgttagtcg aaacattgtt cgacgcctac
    12061 tgccagttga ttaaccaact ctgcgatgac gaaagcgcct ggcaaaagcc gttcgcagat
    12121 atgatgcccg ccagccagcg cgcgatacgc gaacgggtca acgccaccgg cgcccccatt
    12181 cccgaaggct tgctgcatga aggcattttc cgtatcgctc tgcaacagcc gcaggcgctg
    12241 gcggtaacgg acatgcgtta tcagtggaat tatcatgagc tgacagacta tgcccgccgt
    12301 tgcgcgggca ggttaatcga gtgcggggtt cagcccggcg ataatgtggc tatcacgatg
    12361 tcgaaaggcg caggacaact tgttgcggtt ctggccgtcc tgctggccgg ggcggtttac
    12421 gttccggttt cgctggatca gcctgccgca cggcgcgaga aaatctacgc tgacgccagc
    12481 gtccggctgg tgctcatttg tcagcacgac gccagcgccg ggtcagacga tattcccgcc
    12541 cttgcctggc agcaggccat tgaggcggag ccgatcgcca acccggtagt acgcgccccc
    12601 acgcaaccgg cctacattat ctacacctcc ggctctaccg gtacgccgaa aggggtagtc
    12661 atttctcacc ggggagcgct taacacctgt tgcgatatca atacccgcta tcaggttggc
    12721 ccgcatgaca gggtgctggc cctctccgcc ctacattttg atttatcggt ttacgacatt
    12781 tttggcgtac tgcgcgcggg cggcgcgctg gtgatggtga tggaaaatca acggcgcgat
    12841 cctcacgcat ggtgtgagct gatccagcgc catcaggtca cgctctggaa cagcgtcccg
    12901 gcgctgttcg atatgctgct gacctggtgt gaaggtttcg ccgacgccac gccggaaaac
    12961 ctgcgcgcag tgatgctttc cggcgactgg atcgggcttg acctccccgc ccgttatcgg
    13021 gccttccggc cacaaggaca atttatcgcg atgggcggcg ccaccgaggc gtctatctgg
    13081 tctaacgcct gcgaaattca cgacgtcccc gcctactggc gctccatccc ttacggtttt
    13141 ccgctaacca accaacgcta ccgggtggtg gatgaacagg gccgggactg ccctgactgg
    13201 gtgccgggtg aattatggat tggcggcatt ggggtcgcgg aaggctattt caacgatccc
    13261 ctgcgtagcg agcagcaatt tttgacgctc ccggacgagc gctggtatcg caccggcgat
    13321 ctcggctgct actggccaga tggcacaatc gagttcctcg gtcgtcgcga caagcaggtc
    13381 aaagtcggag gatatcgcat cgagctgggc gaaatcgaaa gcgcgctcag ccagctggtg
    13441 ggggtgaaac aagcaaccgt tctggcgatc ggcgaaaaag aaaaaacgct ggcggcatac
    13501 gttgttcctc agggcgaggc tttttgcgtt accgatcatc ggaacccggc actgccgcag
    13561 gcgtggcaca cgcttgcggg aacgttgccc tgttgcgcca tctcgccaga gatctccgca
    13621 gaacaggtag ccgatttcct tcagcatcgc ctgctaaaac tgaagccggg tcacaccgct
    13681 ggcgccgatc ctctccccct gatgaactca ctcgctatcc agccgcgctg gcaggccgtg
    13741 gtggaacgct ggttagcatt tctggtgaca caacggcgac tgaagcccgc tgctgaaggt
    13801 tatcaggtct gcgctggtga agaacgcgag gatgagcacc cgcacttcag cggacatgat
    13861 ttaacgttat cgcaaattct tcgcggtgcc cgtaacgaac tgtcgttact gaacgacgcg
    13921 cagtggtcgc cggaaagcct ggcctttaac catccggcca gcgccccgta tattcaggaa
    13981 ctggcgacaa tttgccaaca gcttgcacag cgcttacagc gcccggtacg cctgcttgag
    14041 gtgggaaccc gcactggccg cgccgcagaa tcgctgttag cacagctcaa cgccggacag
    14101 attgagtatg tcgggcttga gcagagccag gagatgctgc tgagcgcccg gcagaggctc
    14161 gccccctggc ctggcgcccg tctgtccctc tggaatgcag acacgctggc ggcgcacgct
    14221 cactcggcgg acattatctg gcttaataac gccctgcatc gtctgctgcc ggaagatccc
    14281 gggctccttg cgacattaca acagcttgcc gttcccggcg cgctgctcta cgtgatggag
    14341 tttcgccagt taacgccgtc cgccctactc agcacgctcc tgttaaccaa tgggcagccg
    14401 gaggccttgc tgcataacag cgccgactgg gcggcattat ttagcgcggc cggcttcaac
    14461 tgtcagcatg gcgatgaggt cgcggggtta caacgcttcc tcgtacaatg tcctgacagg
    14521 caggtgcgcc gcgatccccg tcaacttcag gccgccctcg ccgggcgtct gccggggtgg
    14581 atggtgccgc aacggatcgt cttcctcgac gccttaccgc tgacagctaa cgggaaaatt
    14641 gactaccagg cgctgaagcg tcgtcatacc cctgaagcgg aaaacccggc cgaagcggat
    14701 ttaccccagg gcgacattga aaaacaggtt gccgccctct ggcagcaact cttatcaact
    14761 ggcaatgtca ccagagaaac cgacttcttc cagcaaggcg gcgatagcct gctggcgacc
    14821 cgtctgaccg ggcaacttca tcaggcaggt tatgaagcgc aattaagcga cctgtttaat
    14881 catccccggc tggcggattt tgccgccacg ctgcggaaaa ccgacgtccc ggtcgaacaa
    14941 ccattcgtcc actcccctga agatcgctac cagccctttg cgcttaccga cgtgcagcag
    15001 gcttacctgg tggggcgtca gccgggcttt gccctgggcg gcgtcggctc acatttcttt
    15061 gttgaatttg aaattgccga tctggacctc acccggctgg agacggtctg gaaccgatta
    15121 atcgcccgcc acgatatgct gcgcgccatc gtgcgtgatg gacagcaaca ggtgctcgaa
    15181 cagacgcccc cctgggtgat acccgcacac accctccata cgcctgaaga ggcgttgcgg
    15241 gtgcgcgaaa aactggcgca tcaggtactc aaccccgaag tatggccggt attcgatctc
    15301 caggtcggat acgtggacgg gatgcctgcc cgcctgtggc tgtgtctgga taacctgttg
    15361 cttgacggtc tgagcatgca gatcctgctg gcggagctgg agcacggcta ccgctacccg
    15421 caacagctgc ttccgccgct gcccgtcacc ttcagggatt atctgcaaca accctcgcta
    15481 cagtcgccca atccagattc tctggcatgg tggcaggcgc agcttgatga tattcctccg
    15541 gcgcctgcgt tgccgctgcg ctgcttgcct caggaggttg aaacaccgcg cttcgcccgc
    15601 ctgaacggcg cactggacag cacgcgctgg catcggctga aaaaacgggc ggctgacgcc
    15661 catctcaccc cgtcggccgt actgttgtcg gtgtggtcaa cggttctctc tgcatggagt
    15721 gcacagcctg acttcacgct taaccttacg cttttcgaca ggcgaccgct gcacccgcaa
    15781 atcaaccaga ttctgggcga tttcacctcg ctgatgctgc tgagctggca tcccggcgaa
    15841 agctggctgc acagcgcgca gtcactacag cagcggctga gccagaacct caaccaccgc
    15901 gatgtgtcag ccatccgcgt gatgcgtcaa ctggcgcaac ggcaaaacgt gccagccgtt
    15961 ccgatgcccg tcgtctttac cagcgcgctg ggctttgagc aggataactt cctcgcccgg
    16021 cgtaatctgc tcaaaccggt ctggggcatc tcccagacgc cgcaggtctg gctcgatcac
    16081 cagatttatg aatccgaagg cgaactgcgc tttaactggg attttgtcgc cgcgctgttt
    16141 cctgccgggc aggtggagcg ccagtttgaa cagtattgcg cattgctaaa ccgaatggcc
    16201 gaggatgaaa gcggctggca actgccgctc gccgcgctgg tgcctcccgt taaacacgca
    16261 gggcaatgcg cagagcgctc accgcgcgta tgccctgagc actctcagcc acacattgcg
    16321 gcggacgaga gcaccgtcag cctgatttgc gacgccttcc gcgaggtggt tggcgagtct
    16381 gtcacgcccg cagaaaactt ctttgaggcg ggcgcaacgt cgctgaatct ggtgcaactg
    16441 cacgttttgt tacaacgtca cgaattttcc accctgacgt tgcttgacct cttcacccac
    16501 ccttctcctg ctgccctggc cgattatctg gccggcgtcg ccacggtgga gaaaacacaa
    16561 cgacctcgcc ctgttcgccg tcgtcagcgg cggatatagc gcgaagcaaa ctgattttcc
    16621 ccggaacgcc atcgcgaacg catggcgttc cattgacttt gtgaatctta ggaaacggga
    16681 ccgattatgg ataacttgcg cttctcttct gcgccgacag cagattccat tgatgcatcg
    16741 atcgctcaac actacccgga ctgcgaacct gtcgcggtta tcggctacgc ctgccatttt
    16801 cctgaatcgc cggatggcga aacgttctgg caaaatctgc tggaaggtcg tgaatgcagc
    16861 cgacgcttta cgcgcgaaga gcttctggcc gtcggtctgg atgccgccat cattgacgat
    16921 cctcattatg tcaatatcgg ttcggtgtta gacaacgccg actgcttcga cgccaccctg
    16981 tttggctatt cgcgacagga agcggagtcg atggacccgc agcagcgcct gtttttgcag
    17041 gcggtctggc atgcgctgga acatgccggt tatgcccccg gcgccgtccc ccataagacc
    17101 ggcgttttcg cctcttcccg gatgagtacc taccccggtc gcgaagcatt gaacgtgaca
    17161 gaagtcgcgc aggtaaaagg tctgcaatct ctgatgggca atgataaaga ctatattgcc
    17221 acccgcgccg cgtacaaact caacctgcac ggcccggcgt tatcggtaca gaccgcctgc
    17281 tccagctcgc tggttgccgt gcatctggcc tgtgaaagcc tgcgcgctgg cgaatccgat
    17341 atggcggttg ccggcggcgt ggcgctctct ttcccccagc aggcaggcta ccgctaccag
    17401 cccggaatga ttttctctcc tgatggtcac tgtcgtccct ttgacgcctc ggctgagggc
    17461 acctgggccg gtaacggtct cggctgcgtg gtgctgcgtc gcctgagaga cgcgctgctg
    17521 tcaggcgatc cgattatctc ggtgatcctc tccagcgcgg tcaacaacga cggcaacaga
    17581 aaggtcggct ataccgcccc ttccgtcgca gggcaacagg cagtcatcga agaggcgtta
    17641 atgctggcgg ccatcgacga caggcaggta ggttacattg aaacccacgg caccggcaca
    17701 ccgctgggcg acgcgattga aattgaagcg ttacgcaacg tctatgcgcc tcgcccgcag
    17761 gatcagcgct gtgcgctcgg ttccgtgaaa agtaacatgg gccatctgga taccgcggcg
    17821 ggcattgccg gactgctgaa aaccgttctg gcagtcagtc gcgggcaaat tcctccctta
    17881 ctgaattttc acacccccaa cccggcgctg aaacttgaag agagcccctt taccataccg
    17941 gtgtcggcac aggcatggca ggacgaaatg cgctatgcgg gcgtctcctc ctttggtatt
    18001 ggcggcacca actgccatat gatcgtcgcc tcgctgcccg acgcgctcaa cgcgcgcctc
    18061 cccaatacgg atagcggcag aaaaagtacc gcgctgctgc tcagcgccgc cagcgacagc
    18121 gcgttgcggc ggctggcgac ggattatgcc ggggcgctga gagagaatgc ggatgccagc
    18181 tctctggcct tcacagccct gcacgcgcgc cgtctcgatc tccccttccg cctggcggcg
    18241 ccattaaacc gtgaaaccgc cgaggcgctc agcgcctggg ccggtgagaa atcgggggcg
    18301 ctggtttaca gcggccacgg cgccagcggc aagcaggtgt ggctgtttac cggccagggc
    18361 tcgcactggc acactatggg ccaaacgatg taccagcact caacggcgtt tgccgacacg
    18421 ctggatcgct gtttttccgc ctgtagcgaa atgctcacgc catcactgcg cgaagcgatg
    18481 tttaaccccg attcggcgca gctggacaat atggcctggg cgcagccggc gattgtcgcg
    18541 tttgaaatcg cgatggcggc gcactggcgt gctgaaggac tgaagccaga cttcgccatt
    18601 gggcattccg tcggtgaatt tgccgctgcc gttgtctgcg gacactatac gattgaacag
    18661 gtcatgccac tggtttgtcg gcgcggcgcg ctaatgcagc agtgcgcaag cggcgcgatg
    18721 gtggcggtat ttgcagacga agacacgctg atgccgctgg ctcgccagtt tgagctggat
    18781 ctcgccgcca acaacggtac gcaacatacg gtattttccg ggccggaagc ccgtctcgcg
    18841 gtattttgcg ccacgctctc gcagcatgac attaactatc gtcgcctgag cgtaaccggt
    18901 gcggcgcact ccgctttact ggagccgata ctcgatcggt tccaggacgc ctgcgcggga
    18961 ctgcacgcgg agccggggca aataccgatt atttccacgc tcaccgccga cgtcattgat
    19021 gagtcaacgc tcaaccaggc ggattactgg cgccgacaca tgcgccagcc ggtgcgtttt
    19081 atccagagta ttcaggtggc gcatcagctc ggcgcccgcg tttttctgga gatggggccc
    19141 gatgcccagt tggttgcttg cgggcagcgc gaataccgcg ataacgcata ctggatagcc
    19201 agcgcccggc gtaacaaaga ggcgagcgat gtcctcaatc aggccctgct ccagctttac
    19261 gctgccggcg tcgccctacc gtgggccgac ctgctggcgg gcgatggaca acgtatcgct
    19321 gcgccatgtt atccgtttga tactgaacgt tactggaaag agcgcgtctc cccggcctgc
    19381 gagcctgccg acgcagcgct gtctgccggg ctggaggtgg cgagtcgcgc cgcgacagcg
    19441 ctcgatctcc ctcgcctgga agcgcttaaa cagtgcgcca cgcgactgca cgccatctac
    19501 gtcgatcaac tggtacaacg ctgtaccggc gatgccattg agaacggcgt ggacgccatg
    19561 accatcatgc gccgtggacg tctgctgccc cgctaccagc agctactcca gcgcctgctg
    19621 aataactgcg tggtcgacgg cgattaccgc tgcaccgacg ggcgatacgt ccgcgcccgc
    19681 cccattgaac atcaacagcg ggaatcactg ctgacggaac ttgccagtta ttgtgaaggt
    19741 tttcaggcta ttcccgacac catcgcccgt gccggcgatc ggttatatga aatgatgagc
    19801 ggcgcggaag aaccggtggc gattatcttc ccgcaaagcg cctccgacgg cgtggaagtg
    19861 ctgtatcagg aattcagctt tggccgctat ttcaaccaaa tcgccgccgg ggtattacgc
    19921 ggcattgtcc agacgcgtca gccccgccag tcgttgcgta ttcttgaagt tggcggcgga
    19981 accggcggca ccaccgcgtg gctgctgccg gaactcaacg gcgttccggc actggagtac
    20041 catttcaccg atatctcggc gctgttcacc cgtcgcgccc agcagaaatt cgccgactat
    20101 gattttgtga agtatagcga gctggatctc gaaaaagagg cgcagtctca gggtttccag
    20161 gcacagtctt acgatcttat cgtggcagcg aacgtgattc acgccacccg ccatattggc
    20221 cgcacgctcg ataatctgcg ccccctgctc aagccgggcg ggcgcctgct gatgcgcaaa
    20281 atcacccagc caatgcgtct gtttgacttc gttttcggcc cgctggttct tccgctacag
    20341 gatctcgacg cccgcgaagg tgagttattc ctcaccaccg ctcagtggca acaacagtgc
    20401 cgccacgccg gattcagcaa agtggcgtgg ctaccgcagg atggcagccc gaccgccggg
    20461 atgagcgaac atatcattct cgccacgctg cccggtcagg cggttagcgc cgtaacattc
    20521 accgcgccat cagaacccgt gttggggcag gcgctgacgg ataacggtga ttatctcgcc
    20581 gactggtctg attgcgcagg tcagcccgaa cagtttaacg cccgctggca ggaggcctgg
    20641 cgtctgcttt cacagcgtca tggcgacgct ctgcctgtgg aaccgccccc cgtcgccgcc
    20701 ccggagtggc tggggaaggt tcgcttaagc tggcaaaacg aagccttttc ccgcggtcag
    20761 atgcgcgttg aagcccgtca tcctgctggc gagtggctgc cgctatcgcc cgccgcgcct
    20821 cttcctgcgc cgcaaacgca ttatcaatgg cgctggacgc ccctcaacgt cgccagcatt
    20881 gaccatccgc ttacctttag cttcagcgcc ggtacgcttg cgcgcagcga cgagctggcg
    20941 caatacggca tcattcacga tccgcacgcc tcttcacgac tgatgattgt tgaggagagc
    21001 gaggatacgc tggccttagc ggagaaagtg atagcagcgc tcaccgccag cgcagccgga
    21061 ttgattgtgg ttactcgccg cgcgtggcga gtcgaggaaa atgaagcact ctctgcatcc
    21121 catcacgcgc tatgggcctt gcttcgcgtc gcggccaacg aacagccgga acggttgctt
    21181 gccgccatcg atctcgccga aaacaccccg tgggaaacgc tgcatcaagg gttgagcgca
    21241 gtctcactat cacagcgctg gctcgccgca cggggtgaca ccctttggct cccttcactg
    21301 gcgcccaata cgggatgcgc agctgaatta ccggcaaacg tgtttaccgg cgatagccgc
    21361 tggcatctgg tgaccggagc gtttggcgga ttaggccgcc ttgccgtgaa ctggctcaga
    21421 gaaaaagggg cgcgacgcat cgccctgctg gcatcgcacg tggatgagtc atggctacgc
    21481 gacgtggagg gcgggcagac gcgcgtctgc cgttgtgatg tgggcgatgc cgggcaactg
    21541 gccacggttc ttgacgatct ggcggccaac ggcggcattg ccggagcgat tcatgccgct
    21601 ggcgtattgg ctgacgcgcc cttgcaggag cttgatgacc accagctggc tgccgttttc
    21661 gcggtaaaag cgcaggcggc aagccagctg ttgcaaaccc tgcgcaacca cgacggacgc
    21721 tatcttattc tctactcttc cgctgccgcc accctcggcg cgccgggtca gagcgcccat
    21781 gcgctgtcct gcggctacct ggacgggctg gcccagcagt tttccaccct tgatgcgccg
    21841 aaaacgctct ctgtcgcctg gggcgcatgg ggagaaagcg gtcgggcggc cacgccggaa
    21901 atgctggcga cgctcgccag ccgaggtatg ggcgcgttaa gcgatgccga aggctgctgg
    21961 cacctggaac aggcggtgat gcgcggcgcc ccgtggcgac tggcgatgcg cgtttttacc
    22021 gacaaaatgc ccccgttaca acaggctctg tttaacatca gcgccacaga aaaagccgca
    22081 acgccggtca ttcctcctgc tgatgacaac gcctttaacg gcagcctgag cgatgaaaca
    22141 gcggtgatgg catggctgaa aaagcggatt gcggttcagc taaggctgag cgatccggcg
    22201 tcactgcatc caaaccagga tctgttgcaa ctcggcatgg actcgctgct cttccttgaa
    22261 ctcagtagcg atattcagca ctacctgggc gtacgcatca atgcggaacg ggcgtggcag
    22321 gatctgtctc ctcatggact cacgcagctt atctgttcta agccagaggc gacgcctgcc
    22381 gtttcgcagc cggaagtgtt gcggcacgac gccgacgagc gttatgcgcc cttccctttg
    22441 acgcccattc agcacgccta ctggctgggg cgaacccacc tcattggcta tggcggcgtc
    22501 gcctgtcacg tcctgtttga gtgggataaa cgccacgatg agttcgatct agccatactg
    22561 gagaaagcat ggaaccagct catcgcacgc cacgatatgt tgcgtatggt ggttgatgcc
    22621 gacgggcagc agcgaatcct ggcgacaacg ccggagtatc acatcccgcg tgacgatctg
    22681 cgcgcgcttt ccccggaaga acagcgcatc gcgctggaaa aacggcggca tgaactgagc
    22741 tatcgcgttt tgcctgccga ccagtggcct ctttttgagc tggtggtcag cgaaatcgac
    22801 gattgccatt accgtctgca tatgaacctc gaccttttgc agtttgatgt gcagagtttt
    22861 aaagtcatga tggacgacct ggcgcaggtc tggcgcggtg aaacgctggc gccgctcgct
    22921 attaccttcc gtgattatgt gatggctgaa caggcgcgcc gaaagacatc ggcatggcac
    22981 gatgcctggg attactggca ggaaaaactg ccgcaactgc ccttagcgcc agagctgccg
    23041 atggttgaga cgcccccgga aacgccacac ttcaccacct tcaaatcgac gatcggcaag
    23101 acagaatggc aggccgtgaa acagcgctgg cagcagcaag gcgtcacacc gtctgccgcg
    23161 ctgctcacgc tgtttgccgc cacccttgag cgctggagcc gtaccacaac atttacgctg
    23221 aacctgacgt tcttcaatcg ccagccgatc catccgcaaa tcaaccagtt gattggtgat
    23281 tttacctccg tcacgctggt tgattttaac ttctcagcgc cagtgacgtt gcaagagcag
    23341 atgcaacaga cccaacagcg cctctggcaa aatatggcgc acagtgaaat gaacggtgtt
    23401 gaggtgatcc gtgagctggg ccgcctgcgc ggatcacaac gtcaaccgct gatgccggta
    23461 gtgtttacca gtatgctggg gatgacgctg gaaggcatga ctatcgatca ggcgatgagc
    23521 catctgttcg gcgaaccctg ctatgtattc acgcaaacgc cgcaggtctg gctggatcat
    23581 caggtcatgg agagcgacgg cgagttgatg tttagctggt actgcatgga caacgtgctg
    23641 gaacccggcg ctgccgaggc gatgtttaat gactattgag ccatcctgca agccgtcatc
    23701 gccgcccctg aaagcctgaa gactctcgcc agcggcatcg ccgggcacat tccccgccga
    23761 cgctggccgc tgaacgcgca ggcggactac gacctgcggg atattgagca ggcgacgctc
    23821 gaataccccg gcatccggca ggccagagcg gaaataaccg aacagggcgc gttgacgctg
    23881 gatatcgtga tggccgacga tccttcgcca tcagcggcga tgcctgatga gcacgaactt
    23941 acccaactgg cgctgccgtt gcctgagcag gcgcagcttg atgagctgga ggcgacctgg
    24001 cgctggctgg aggcgcgtgc gctacagggg atcgcggcta cgctaaatcg tcacggcctg
    24061 tttaccacgc cggagatcgc ccatcgcttt agcgcaatag tacaggcgct gtccgcgcaa
    24121 gcgtctcacc agcgtctgct gcgccagtgg ctacagtgtc tgacggaaag agagtggtta
    24181 atccgcgaag gtgaaagctg gcgctgccgc attccgctca gcgagattcc tgagcctcag
    24241 gaagcgtgcc cgcaaagcca atggagccag gcgctggcgc agtatctgga aacctgcatc
    24301 gcccggcacg acgccctctt ctccgggcag tgttctccgc tggaattgct gttcaacgag
    24361 cagcatcgcg ttaccgacgc gctgtatcgc gacaaccccg ccagcgcctg tctgaatcgc
    24421 tataccgcgc agattgccgc cttgtgcagc gcagaacgga ttctggaggt tggcgccgga
    24481 accgcagcca ctaccgcgcc ggtgctgaag gccacgcggc agtcgtacca cttcacggac
    24541 gtctccgcgc agttcctcaa tgacgccaga gcccgtttcc atgatgaatc gcaggtgtct
    24601 tatgccttgt tcgacatcaa ccagccgctg gatttcaccg cccacccgga ggcgggttac
    24661 gacctgatcg ttgccgtcaa tgtgctccac gacgccagcc atgtcgtcca gacgttgcgc
    24721 agattaaaac tgttgctgaa agccggcgga cgtttgctga tcgttgaagc gacggagcga
    24781 aacagcgtat tccagctggc gagcgtgggc tttattgagg gattaagcgg ataccgcgat
    24841 ttccgccgcc gggatgagaa accgatgctc acccgctccg catggcagga ggttctcgtt
    24901 caggccgggt ttgcaaacga gctggcgtgg cccgcgcagg aatcgtcgcc gctgcgccag
    24961 catctgctgg tagcgcgttc gcctggcgta aatcgcccgg ataaaaaagc cgtgagccgc
    25021 tatttacagc agcgctttgg caccggtctg cccattttac agatccggca aagagaagcg
    25081 ttatttacgc cgctgcatgc cccgtctgat gcgccgactg agccagccaa acccacgcca
    25141 gttgccgggg ggaattcggc gctggaaaaa caggtggctg aactctggca atcgctgctg
    25201 tctcgccccg tggcaaggca tcacgacttt ttcgaactgg gcggcgacag cctgatggcg
    25261 acaaggatgg tcgcgcagct aaaccggaga gggattgcca gggctaacct tcaggatctg
    25321 ttcaaccatt cgacgctgag cgacttctgc gcccatctac aggcggctac gtcaggagag
    25381 gacaacccga tacccctttg ccagggcgac ggtgaggaaa ccctgtttgt cttccacgct
    25441 tcagacggcg atatcagcgc ctggctgccg ctcgctagcg cgttgaacag gcgcgttttc
    25501 ggcctgcaag caaaatcgcc gcagcgcttt gctacgctcg accagatgat cgatgagtat
    25561 gtcgggtgca tccgtcgtca gcagcctcac ggcccttatg tgctggcggg ttggtcgtat
    25621 ggcgcgtttc ttgcggcggg cgccgcacag cgcctgtacg ccaaaggcaa gcaggttcgg
    25681 atggtgttaa tcgatcccgt gtgccgacag gatttctgtt gcgaaaaccg ggcggccctg
    25741 ctgcgcctgt tagccgaagg agaaacgcct ctggcactgc ccgaacattt cgaccagcag
    25801 acgcccgaca gccagcttgc cgactttatc agcctcgcta aaacggccgg tatggtgtcg
    25861 caaaacctga cgctgcaagc ggcagaaacg tggctcgaca acatcgcgca tctgctgcgt
    25921 ttactgactg agcatacgcc gggcgaaagc gttccggtcc cctgtctcat ggtgtatgcc
    25981 gccgggagac ccgcgcgctg gacgccagca gaaaccgagt tgcagggctg gataaacaac
    26041 gccgacgacg ctgtgattga agccagccac tggcaaatca tgatggaagc cccccacgtt
    26101 caggcttgtg cgcaacacat tacgcgctgg ctttgcgcaa cctcaacgca accggagaac
    26161 acgttatgat gccgtccgcc tccccaaaac aacgcgtact gattgtgggc gccaaatttg
    26221 gcgaaatgta cctgaatgcc tttatgcagc ccccggaggg gctggaactg gtcggcctgc
    26281 tggcgcaggg aagcgcccgt tcaagagagc tggctcatgc gtttggcatt ccgctgtata
    26341 cctcgccgga acagataacc aggatgccgg atatcgcctg tattgtcgtg cgttcgaccg
    26401 tcgccggagg aaccggtacg cagcttgcca gacactttct gacgcgcggc gtacacgtca
    26461 ttcaggagca tcccctccac ccggatgaca tcagttcctt acaaacgctg gcgcaggaac
    26521 agggctgctg ctattgggta aacacgtttt atccgcatac gcgcgcggga cgcacatggc
    26581 ttcgcgatgc gcagcagctg cgtcgctgcc tggctaagac gccgccggta gtccacgcca
    26641 ccaccagccg acagttgctc tactccactc tggatttgtt gttgctggct ctgggcgttg
    26701 atgccgccgc cgtggagtgc gacatggttg gcagctttag cgattttcac tgtctgagac
    26761 tcttctggcc ggagggagaa gcctgcctgc tgcttcagcg ctatctcgat cctgacgatc
    26821 cagatatgca tagccttatc atgcaccgcc tgctgctggg ctggccggaa ggtcatcttt
    26881 cgctggaggc gagctacggc cccgtcatct ggtcatccag tctgtatgtc gcagaccatc
    26941 agcagaaccc ccacagtctc tggcgcagac cggagatcct gcgcgatctg ccgggtctga
    27001 cgcgcagcgc ggcgcccctg agctggcgcg actgctgcga aaccgtcggg ccggagggcg
    27061 tcagctggtt gctacaccag ttgcgcagca atctggcggg cgaacatcca ccagaggcct
    27121 gccaaagcgt acatcagatc gcgttatcac gtttatggca gcagatttta cgtaagaccg
    27181 gcaatgcgga gatcagacgt ctgacgccgc cgcaccacga ccggttagcc ggtttctaca
    27241 acgatgatga taaggaggcg ctgtgacgca atctgcaatg tgcatcccgc tgtggcccgc
    27301 ccggaacggc aatactgcgc atctggtcat gtgccctttc gctggcggca gcagtagcgc
    27361 gtttcgccac tggcaagctg agcaactggc tgattgcgcg ctttctctgg tgacctggcc
    27421 ggggcgcgat cgccttcgcc atctggaacc gctcagaagc attacacaac tggcggcact
    27481 gctggcgaac gagctggaag catccgtatc gcctgacacg ccgcttttac tcgccgggca
    27541 cagcatgggg gcgcaggtgg cgtttgaaac ctgccgactt ctggagcaac gggggcttgc
    27601 gccacaagga ctgattattt ccgggtgcca tgccccgcat ctgcattctg aacgccagct
    27661 cagccatcgc gatgatgccg actttatcgc tgagctgata gacattggcg gatgttctcc
    27721 tgaactgcgg gaaaaccagg aattaatgtc gctgtttctt cctcttctgc gcgctgattt
    27781 ttacgccacc gagagctatc actacgactc gcccgacgtc tgtccgccgc tgcgcacgcc
    27841 tgcgctgtta ttgtgcggca gccacgatcg cgaagcctcc tggcagcagg tcgatgcctg
    27901 gcgtcagtgg ctgagccacg ttacaggccc ggtggtgatt gacggcgatc acttctatcc
    27961 cattcaacaa gcccggtcct tttttacgca gattgtccgc cattttcccc acgcattttc
    28021 tgcaatgacc gcgtggcaaa aacagcccag tacttcagaa aggtgacgca tgaattcttc
    28081 ctttgaatct ctgattgaac agtatccctt acccattgcc gaacagttgc gccactgggc
    28141 ggcccgttat gcctcgcgaa ttgccgtcgt tgatgcaaag gggtcgttaa cctacagcgc
    28201 gcttgatgca caagttgacg aacttgccgc aggtctgtca tcactgggtt tgcgttcggg
    28261 ggagcatgta attgtgcagc ttcccaacga caacgcgttt gttaccctgc tgttcgcctt
    28321 gttaagactg ggcgttatcc ccgtgctggc gatgccctcg caacgggcgc tggatatcga
    28381 cgcgctgatt gagctggcgc aacccgtcgc ttacgttatt cacggggaaa accacgcaga
    28441 gctggcccga cagatggcac acaaacacgc ctgcttgcgt catgttctgg tcgctggaga
    28501 gaccatgagc gacgatttta cgccgctctt ctcccttcac ggtgagcgac agcctgatgt
    28561 ttccaccacc gcgttgttgt tgctctcagg cggcacaacc ggcacgccca aactcatccc
    28621 gcgccgacat gccgactata gctataactt cagcgcttct gctgaactgt gcggcatcag
    28681 ccaacagagc gtgtatctcg ccgtcctccc ggtggcgcat aactttccgc tggcctgccc
    28741 cggtattctg ggaacgcttg cctgcggcgg aaaagtggtg ctgaccgaca gcgccagctg
    28801 tgatgaggtg atgcctttaa tcgcgcagga aagagtgact cacgtcgccc tggttccggc
    28861 gctggcgcaa ttatgggtgc aggccaggga gtgggaagac agcgaccttt cgtcgctgcg
    28921 cgtcattcag gcaggcggcg cccggctcga cccgacgctt gctgagcagg ttatcgccac
    28981 ctttgactgt accctgcaac aggttttcgg tatggcggaa ggcctgctct gttttacccg
    29041 gctggacgat ccgcatgcca ccattctcca cagccagggg cgcccgttgt cccctctgga
    29101 tgaaatctgc atcgttgatc aagacgagaa cgacgtcgcg ccgggcgaaa ccgggcaatt
    29161 gttaacgcgc ggcccttata ccatttcggg ctattaccac gcccctgccc acaacgcgca
    29221 ggcctttacc gcgcaagggt tttaccgcac aggcgacaat gtcaggctgg atgaggtggg
    29281 gaacctgcac gttgagggac gcataaaaga gcagatcaac cgcgccggag aaaaaatagc
    29341 cgcggctgaa gtggaatcgg cactgctgcg tttagcggaa gtgcaagatt gcgcggtggt
    29401 cgccgcgccg gacacgctgc ttggcgagcg gatttgcgcg tttatcatcg cgcagcaggt
    29461 gccaactgac tatcagcagt tgcgtcaaca actgacccgt atggggctca gcgcgtggaa
    29521 aattcctgac caaatcgagt ttctggacca ctggccgctc accgccgtcg gcaagataga
    29581 caaaaaacgc ctgacggctc tcgccgtcga ccgttatcgc cattctgccc aataagcgca
    29641 aaccgacccg aaacaggttg aaataaaccc gtttcgggta gcaccactat tagaaatagt
    29701 tatcattttc aattcaccat tgtcggtatt tttggcgttt cgccgtctta cagggactca
    29761 caacaatgaa aatgacacgg ctttatcctc tggccttggg gggattattg ctccccgcca
    29821 ttgctaatgc ccagacttca cagcaagacg aaagcacgct ggtggttacc gccagtaaac
    29881 aatcttcccg ctcggcatca gccaacaacg tctcgtctac tgttgtcagc gcgccggaat
    29941 taagcgacgc cggcgtcacc gccagcgaca aactccccag agtcttgccc gggctcaata
    30001 ttgaaaatag cggcaacatg cttttttcga cgatctcgct acgcggcgtc tcttcagcgc
    30061 aggacttcta taaccccgcc gtcaccctgt atgtcgatgg cgtccctcag ctttccacca
    30121 acaccatcca ggcgcttacc gatgtgcaaa gcgtggagtt gctgcgaggc ccacagggaa
    30181 cgttatatgg caaaagcgct cagggcggga tcatcaacat cgtcacccag cagccggaca
    30241 gcacgccgcg cggctatatt gaaggcggcg tcagtagccg cgacagttat cgaagtaagt
    30301 tcaacctgag cggccccatt gaggatggcc tgctgtacgg cagcgtcacc ctgttacgcc
    30361 aggttgatga cggcgacatg attaaccccg cgacgggaag cgatgactta ggcggcaccc
    30421 gcgccagcat agggaatgtg aaactgcgtc tggcgccgga cgatcagccc tgggaaatgg
    30481 gctttgccgc ctcacgcgaa tgtactcgcg ccacccagga tgcctatgtg ggatggaatg
    30541 atattaaggg ccgtaagctg tcgatcagcg atggttcacc agacccgtac atgcggcgct
    30601 gcactgacag ccaaaccctg agtgggaaat acaccaccga tgactgggtt ttcaacctga
    30661 tcagcgcctg gcagcagcag cattattcgc gcaccttccc ttccggttcg ttaatcgtca
    30721 atatgcctca gcgctggaat caggatgtgc aggagctgcg cgccgcaacc ctgggcgatg
    30781 cgcgtaccgt tgatatggtg tttgggctgt accggcagaa cacccgcgag aagttaaatt
    30841 cagcctacga catgccgaca atgccttatt taagcagtac cggctatacc accgctgaaa
    30901 cgctggccgc atacagtgac ctgacctggc atttaaccga tcgttttgat atcggcggcg
    30961 gcgtgcgctt ctcgcatgat aaatccagta cacaatatca cggcagcatg ctcggcaacc
    31021 cgtttggcga ccagggtaag agcaatgacg atcaggtgct cgggcagcta tccgcaggct
    31081 atatgctgac cgatgactgg agagtgtata cccgtgtagc ccagggatat aaaccttccg
    31141 ggtacaacat cgtgcctact gcgggtcttg atgccaaacc gttcgtcgcc gagaaatcca
    31201 tcaactatga acttggcacc cgctacgaaa ccgctgacgt cacgctgcaa gccgcgacgt
    31261 tttataccca caccaaagac atgcagcttt actctggccc ggacgggatg cagacattaa
    31321 gcaatgcggg taaagccgac gccaccggcg ttgagcttga agcgaagtgg cggtttgcgc
    31381 caggctggtc atgggatatc aatggcaacg tgatccgttc cgaattcacc aatgacagtg
    31441 agttgtatca cggtaaccgg gtgccgttcg taccacgtta tggcgcggga agcagcgtga
    31501 acggcgtgat tgatacgcgc tatggcgcac tgatgccccg actggcggtt aatctggtcg
    31561 ggccgcatta tttcgatggc gacaaccagt tgcggcaagg cacctatgcc accctggaca
    31621 gcagcctggg ctggcaggcg actgaacgga tgaacatttc cgtctatgtc gataacctgt
    31681 tcgaccgtcg ttaccgtacc tatggctaca tgaacggcag cagcgccgtc gcgcaggtca
    31741 atatgggtcg caccgtcggt atcaatacgc gaattgattt cttctgatta ttgtaaaagg
    31801 gataccgaaa aggtatccct tttacaccac tagttaaaac cagtaactca gcagagtcgc
    31861 aaaaaatatt aatccatagt gattatttaa aaaatgaaat tgcgattagg at
//
LOCUS       DAZFRJ010000037        31728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000037 DAZFRJ010000000
VERSION     DAZFRJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.037"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(165..1052)
                     /gene="rarD"
                     /locus_tag="ACHKUN_001520"
     CDS             complement(165..1052)
                     /gene="rarD"
                     /locus_tag="ACHKUN_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO6659806.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1104..1571)
                     /gene="yigI"
                     /locus_tag="ACHKUN_001521"
     CDS             complement(1104..1571)
                     /gene="yigI"
                     /locus_tag="ACHKUN_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO6659807.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1736..2605
                     /gene="pldA"
                     /locus_tag="ACHKUN_001522"
     CDS             1736..2605
                     /gene="pldA"
                     /locus_tag="ACHKUN_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO6659808.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2738..4567
                     /gene="recQ"
                     /locus_tag="ACHKUN_001523"
     CDS             2738..4567
                     /gene="recQ"
                     /locus_tag="ACHKUN_001523"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO6659809.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4631..5251
                     /gene="rhtC"
                     /locus_tag="ACHKUN_001524"
     CDS             4631..5251
                     /gene="rhtC"
                     /locus_tag="ACHKUN_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO6659810.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LEWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5313..5933)
                     /gene="rhtB"
                     /locus_tag="ACHKUN_001525"
     CDS             complement(5313..5933)
                     /gene="rhtB"
                     /locus_tag="ACHKUN_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO6659811.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6044..7066
                     /gene="pldB"
                     /locus_tag="ACHKUN_001526"
     CDS             6044..7066
                     /gene="pldB"
                     /locus_tag="ACHKUN_001526"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO6659812.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7074..7874
                     /gene="yigL"
                     /locus_tag="ACHKUN_001527"
     CDS             7074..7874
                     /gene="yigL"
                     /locus_tag="ACHKUN_001527"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO6659813.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7950..8849
                     /gene="bioP"
                     /locus_tag="ACHKUN_001528"
     CDS             7950..8849
                     /gene="bioP"
                     /locus_tag="ACHKUN_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO6659814.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8737..9690)
                     /gene="metR"
                     /locus_tag="ACHKUN_001529"
     CDS             complement(8737..9690)
                     /gene="metR"
                     /locus_tag="ACHKUN_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO6659815.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9808..12069
                     /gene="metE"
                     /locus_tag="ACHKUN_001530"
     CDS             9808..12069
                     /gene="metE"
                     /locus_tag="ACHKUN_001530"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO6659816.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12108..12923)
                     /gene="ysgA"
                     /locus_tag="ACHKUN_001531"
     CDS             complement(12108..12923)
                     /gene="ysgA"
                     /locus_tag="ACHKUN_001531"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO6659817.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13185..13946
                     /gene="udp"
                     /locus_tag="ACHKUN_001532"
     CDS             13185..13946
                     /gene="udp"
                     /locus_tag="ACHKUN_001532"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO6659818.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14087..15514
                     /gene="rmuC"
                     /locus_tag="ACHKUN_001533"
     CDS             14087..15514
                     /gene="rmuC"
                     /locus_tag="ACHKUN_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO6659819.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15609..16364
                     /gene="ubiE"
                     /locus_tag="ACHKUN_001534"
     CDS             15609..16364
                     /gene="ubiE"
                     /locus_tag="ACHKUN_001534"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO6659820.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16378..16983
                     /gene="ubiJ"
                     /locus_tag="ACHKUN_001535"
     CDS             16378..16983
                     /gene="ubiJ"
                     /locus_tag="ACHKUN_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO6659821.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16980..18620
                     /gene="ubiB"
                     /locus_tag="ACHKUN_001536"
     CDS             16980..18620
                     /gene="ubiB"
                     /locus_tag="ACHKUN_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO6659822.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18699..18968
                     /gene="tatA"
                     /locus_tag="ACHKUN_001537"
     CDS             18699..18968
                     /gene="tatA"
                     /locus_tag="ACHKUN_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO6659823.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18972..19487
                     /gene="tatB"
                     /locus_tag="ACHKUN_001538"
     CDS             18972..19487
                     /gene="tatB"
                     /locus_tag="ACHKUN_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO6659824.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19490..20266
                     /gene="tatC"
                     /locus_tag="ACHKUN_001539"
     CDS             19490..20266
                     /gene="tatC"
                     /locus_tag="ACHKUN_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO6659825.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20308..21090
                     /gene="tatD"
                     /locus_tag="ACHKUN_001540"
     CDS             20308..21090
                     /gene="tatD"
                     /locus_tag="ACHKUN_001540"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO6659826.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21087..21575)
                     /gene="rfaH"
                     /locus_tag="ACHKUN_001541"
     CDS             complement(21087..21575)
                     /gene="rfaH"
                     /locus_tag="ACHKUN_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO6659827.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21742..23235
                     /gene="ubiD"
                     /locus_tag="ACHKUN_001542"
     CDS             21742..23235
                     /gene="ubiD"
                     /locus_tag="ACHKUN_001542"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO6659828.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23281..23982
                     /gene="fre"
                     /locus_tag="ACHKUN_001543"
     CDS             23281..23982
                     /gene="fre"
                     /locus_tag="ACHKUN_001543"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO6659829.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24174..25337)
                     /gene="fadA"
                     /locus_tag="ACHKUN_001544"
     CDS             complement(24174..25337)
                     /gene="fadA"
                     /locus_tag="ACHKUN_001544"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO6659830.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25347..27536)
                     /gene="fadB"
                     /locus_tag="ACHKUN_001545"
     CDS             complement(25347..27536)
                     /gene="fadB"
                     /locus_tag="ACHKUN_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO6659831.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27726..29057
                     /gene="pepQ"
                     /locus_tag="ACHKUN_001546"
     CDS             27726..29057
                     /gene="pepQ"
                     /locus_tag="ACHKUN_001546"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO6659832.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29057..29671
                     /gene="yigZ"
                     /locus_tag="ACHKUN_001547"
     CDS             29057..29671
                     /gene="yigZ"
                     /locus_tag="ACHKUN_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO6659833.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29710..31161
                     /gene="trkH"
                     /locus_tag="ACHKUN_001548"
     CDS             29710..31161
                     /gene="trkH"
                     /locus_tag="ACHKUN_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO6659834.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFLVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31173..31718
                     /gene="hemG"
                     /locus_tag="ACHKUN_001549"
     CDS             31173..31718
                     /gene="hemG"
                     /locus_tag="ACHKUN_001549"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO6659835.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7334 a         8093 c         8703 g         7598 t
ORIGIN      
        1 aatccttggt agatatacga tgtctctagg atggagtaag tactggggag ttttgggggt
       61 atttcctgta acaaatattg ttctttttct tatcttaatt tttttcaaaa aaagataaat
      121 aagtcagtgg gtaggctctt taaacggcct acccatgtta attcttaact ttttctacgc
      181 tgcgtataaa tcgcatccat cacaaaaatt gccagcgcca cccaaataaa ggcgaaagtc
      241 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
      301 gtcgggccaa tgtactggaa aaagcctaac gttgagagac gcaagcgcgt ggcagcggcg
      361 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
      421 gacatcgggt tttgccccat atggctggtt gagctgtcgg caatagcaaa caggtaaatt
      481 gccgccacgg gcagcagcca catggtttcg attaacatgc cggtttgcgc ttcaacggca
      541 atcttcttgc gtaccagacc gtagaaggca aaactaaatg ccagtcccag cgcgataata
      601 ggtagcgaac caaaagtcca cagctggact aacacgccac atatcgccag aatcaccgcc
      661 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
      721 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
      781 ataaacagta gccagttgcc accaatcagc acggcagaga ctgccagcat aaaaattttc
      841 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
      901 accataaaga aaaacgacca gatcacgcga tgcgtcagga tttcatcggc aggcacgtag
      961 taaatcaact tgaagtacgc tggcgctata ccccaaataa aataagcggc aagagcgagt
     1021 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     1081 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     1141 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     1201 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     1261 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     1321 tggcgggtta aggtacttcc cacgcacacc agacctgcgg cgacatccag cgctgacgca
     1381 ataaccccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcattgg ctgattttta
     1441 aaggccagct gtgcgaactc tttttcgtaa cgctccagct ccatccctaa cgcgcggtta
     1501 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     1561 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     1621 tgttgttggt ttctacttta ggaagggata actaacggct atggagttaa gtatgtaaaa
     1681 tagcccgcag aaaaatattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     1741 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     1801 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     1861 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcattta
     1921 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     1981 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     2041 aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     2101 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     2161 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     2221 cgactctaac gggcgttccg acccgacctc ccgcagctgg aaccgccttt atactcgcct
     2281 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     2341 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     2401 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     2461 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     2521 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtggg
     2581 ggttatgcta aacgatttgt tttgatgaac ggttgagtgg ttggcaaatc tggaacccag
     2641 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     2701 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     2761 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     2821 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg tcgtcatgcc
     2881 caccggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     2941 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tgcaagccaa
     3001 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     3061 gacaggctgc cgcaccgggc aaattcgtct gctttatatc gccccggaac gcctgatgct
     3121 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg ttgatgaagc
     3181 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     3241 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     3301 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     3361 tgaccgtccg aatattcgct acatgctgat ggagaagttc aaaccgctcg atcagttgat
     3421 gcgctacgtg caggaacagc gcggtaagtc cggcattatc tactgcaaca gccgggcgaa
     3481 agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg cctatcatgc
     3541 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgacctgca
     3601 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     3661 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     3721 gcgtgatggc ctgcccgcgg aagcgatgct gttttacgat ccggccgata tggcgtggct
     3781 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgaac gtcataaact
     3841 caatgcgatg ggcgcttttg ccgaagcgca aacttgccgt cgtctggtac tgctgaacta
     3901 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     3961 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     4021 gcgttttggt atgggctatg tggtggaagt gattcgcggc gctaataacc agcgtatccg
     4081 cgactatggt catgacaaac taaaagtcta cggtatgggc cgtgataaaa gtcatgaaca
     4141 ttgggtgagc gtgatccgcc agctgattca cctcggtctg gtgacgcaaa atattgccca
     4201 gcattctgcc ctacaactga cagaggctgc gcgtccggtg ctgcgcggcg aatcctcttt
     4261 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     4321 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgatagccga
     4381 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     4441 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     4501 ggaacgcttt ggcaaaccgt ttatggcgct gattcgtgcg catgttgatg gcgatgacga
     4561 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     4621 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     4681 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     4741 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgct
     4801 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtggg
     4861 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     4921 ggcggtttct gcacctgcgc cacaggtcga gctggcgaaa agtgggcgca gtttcctgaa
     4981 aggtttactg accaatctcg ctaatccgaa agcgattatc tactttggct cggtgttctc
     5041 attgtttgtc ggtgataacg ttggcactac cgcgcgctgg ggcatttttg cgctgatcat
     5101 tgtcgaaacg ctggagtggt ttaccgtcgt tgccagcctg tttgccctgc cgcaaatgcg
     5161 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     5221 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     5281 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     5341 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     5401 cctttaatcc atagagcaat ccgttgagca agggtggcgt aaccgatcat cacaataata
     5461 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     5521 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     5581 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaagg
     5641 tcaattgcac cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     5701 gcccacttca acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     5761 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     5821 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     5881 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     5941 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     6001 ctaaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     6061 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     6121 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     6181 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     6241 ctacgtaaaa tatgcggaac tggcctatga cctgttccat ttggggtttg atgtcttaat
     6301 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     6361 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     6421 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     6481 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     6541 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     6601 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     6661 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     6721 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     6781 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgccaacgc ttctcttaca
     6841 agctgaagag gaacgcgtgg tggataaccg catgcatgac cgtttttgtg aactccgcac
     6901 cgccgcgggc catcctgtcg aaggaggacg gccgttggta attaaaggtg cttaccatga
     6961 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     7021 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     7081 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     7141 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     7201 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     7261 ttacctccaa tggtgcgcgc gttcacgatc tggatggtaa tctgattttt gctcataacc
     7321 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     7381 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     7441 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc tggattactg gagccggaag
     7501 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     7561 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
     7621 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
     7681 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
     7741 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gaccttcatc
     7801 ccgagctgga agtgattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
     7861 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
     7921 gatactcgtt aatcaaagag ttttccattg tggcgctact tatcatcacc acgattctgt
     7981 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
     8041 cggtgctggt gcgcgttggc ctggcggcac tcgtttttct gccgtttctg cgtacccgtg
     8101 gcaatagcct gaaaacggtc ggcctgtata tgttggtggg cgcgatgcag ctcggcgtga
     8161 tgtatatgct gagtttccgc gcgtatctct acctgacggt ctccgaactg ctgctgttca
     8221 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
     8281 gctggggcta tgcctttagc gccttgttgg cggtgattgg tgccgggatt attcgctatg
     8341 atcaggtcac cgaccatttc tggactggct tgctgctggt gcaactctcc aatatcactt
     8401 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccacagcata
     8461 acgcctttgc gtggttctat cttggcgcgt ttctggtggc ggtaattgca tggttcttgc
     8521 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
     8581 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
     8641 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
     8701 ctatctggca ccaacagccg cactggccaa cgttcattac aggcgcgctg gtgatcctgg
     8761 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
     8821 gcaggcgtga ttgcgcgctg agcgaataaa tgcttctgta atcggctgac gctgctcgcc
     8881 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
     8941 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
     9001 cgcggcaacc atctgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
     9061 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
     9121 caacagcgtc tcgctggcga gatcttccgg tgtaattcgc gttttcgccg ccagtggatg
     9181 gtcaggtgct aacaccagac gcacttcata gtcgaacatc ggcgaataat gcaggccact
     9241 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
     9301 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
     9361 attttctaac gcgggcgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
     9421 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctaatttgcg gcagcacctg
     9481 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
     9541 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
     9601 ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag ccgcagttcc gcaacgcttg
     9661 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
     9721 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
     9781 tactaaaaat tccataaggg gcatataatg acaattctta atcacaccct cggtttccct
     9841 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
     9901 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
     9961 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgttctg
    10021 accaccagtc tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    10081 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    10141 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggaattcgtt
    10201 aaaggccaac agttcaaact gacctggacg cagctgctgg aggaagtgga cgaggcgctg
    10261 gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    10321 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    10381 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    10441 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    10501 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    10561 gacacgatta ctgcgctgcc tgttcagggt ctgcatgttg acctcgtaca tggtaaagat
    10621 gacgttgctg aactgcacaa gcgcctgcct tctgactggt tgctgtctgc gggtctgatc
    10681 aatggtcgta acgtctggcg cgccgatctt accgagaaat atgcgcaaat taaggacatt
    10741 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    10801 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    10861 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    10921 gcagagtgga gcgccccgat tcaggcacgt cgtcactcta cccgcgtaca taatccggcg
    10981 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    11041 gtgcgtgccg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    11101 ggttctttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    11161 ctcgacgcca ataactaccg cacgggcatt gcggaacata tcaggcaggc cattgttgaa
    11221 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    11281 gaatactttg gcgagcacct cgacgggttt gtctttacgc aaaacggttg ggtacagagc
    11341 tacggttccc gctgcgtgaa gccaccgatt gttattggtg acgttagtcg cccggcaccg
    11401 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    11461 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    11521 accatcgcca aacagattgc gctggcgctg cgtgatgagg tggccgatct ggaagcggca
    11581 gggattggta tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc actgcgtcgt
    11641 agcgactggg acgcctatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    11701 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    11761 gattccattg cggcgctgga tgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    11821 gagttgctgg agtcgtttga agagtttgat tatccgaatg aaatcggtcc aggcgtctat
    11881 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccctgct gaagaaagcg
    11941 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccag actgtggcct gaaaacgcgc
    12001 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    12061 ctggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    12121 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    12181 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtagacgata
    12241 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    12301 ggaatgctgt tatcctgacc gccatataag ccgagaaccg gcgcgttaag atcggttgcg
    12361 atatcaacag gttgtttcgg tgaattcagc gacttatcgc ccgtcagttt gccgtaccac
    12421 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    12481 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    12541 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    12601 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    12661 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    12721 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    12781 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    12841 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    12901 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    12961 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    13021 cctgcagaat gaagagtgat ttatgtgatt tgcatcactt ttggtgggta aatttatgca
    13081 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    13141 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    13201 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    13261 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    13321 ctcaccgcga attcaccacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    13381 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    13441 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    13501 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    13561 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    13621 ccattggcgc gacaactcac gttggcgtga cagcttcttc tgataccttc tacccaggtc
    13681 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    13741 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaaccctg ctgaccatgt
    13801 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    13861 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    13921 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    13981 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    14041 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    14101 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    14161 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    14221 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gcgagttact
    14281 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    14341 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    14401 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    14461 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    14521 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    14581 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    14641 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    14701 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    14761 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    14821 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    14881 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    14941 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    15001 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt cactggcgct
    15061 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    15121 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    15181 ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    15241 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    15301 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    15361 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    15421 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    15481 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    15541 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    15601 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    15661 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    15721 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    15781 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    15841 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    15901 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    15961 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    16021 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    16081 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    16141 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    16201 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    16261 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    16321 tgacggcggg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    16381 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    16441 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    16501 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    16561 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    16621 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    16681 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    16741 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    16801 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    16861 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    16921 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    16981 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    17041 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    17101 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    17161 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    17221 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    17281 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    17341 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    17401 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    17461 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    17521 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    17581 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    17641 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    17701 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    17761 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    17821 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    17881 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    17941 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    18001 cagagctaca cgtcgattca ggctgggtgc caccagatac caacgttgaa gagttcgaat
    18061 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    18121 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    18181 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    18241 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    18301 tcggtattcc tgcgctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    18361 tgccagaact gcctgaattg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    18421 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    18481 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    18541 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    18601 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    18661 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    18721 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    18781 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    18841 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    18901 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    18961 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    19021 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    19081 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    19141 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    19201 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    19261 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    19321 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    19381 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    19441 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    19501 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    19561 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgata tctatcacct
    19621 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    19681 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    19741 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    19801 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gtatggcatt
    19861 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    19921 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    19981 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    20041 ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    20101 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    20161 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    20221 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    20281 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    20341 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    20401 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    20461 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    20521 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    20581 tgtggtctcg actttaaccg caacttttcg acgccggaag agcaggaacg cgcttttgtt
    20641 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    20701 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    20761 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    20821 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    20881 ttgattccgg cggagaaatt gctgatcgaa actgatgcgc cgtatctgct cccgcgcgat
    20941 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    21001 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgctaat
    21061 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    21121 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    21181 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    21241 ggataagggg ttgccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatc
    21301 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca cgaagtggct gacaccgcgg
    21361 gtcgcgttga tagtcgtggt atgaatcact tctggatcaa attcgacaaa caggtagttg
    21421 ggaaacaatg gctcactgac tgcagtacgt tttccacgca cgattttttc cagggtgatc
    21481 atcggtgcca ggcaattcac agcctgtctt tcgaggtgtt cctgggcacg ttgaagttgc
    21541 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    21601 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    21661 gtcagagttc acgctaattt aacaaattta cagcatcgca aagatgaacg ccgtataatg
    21721 ggcgtagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcttg
    21781 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    21841 gaaatcactg aaattgctga ccgcactttg cgtgccggtg ggcctgcgct gttgttcgaa
    21901 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    21961 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    22021 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    22081 tttaagcaag tattgaacat gccgacaaag cggctgcgtg gtgcgccctg ccaacaaaaa
    22141 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    22201 gatgccgcgc cgctgattac ctgggggctg acagtgacgc gcggcccgca taaagagcgg
    22261 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    22321 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    22381 cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca cgattctcgg tgcagtcacc
    22441 cccgttccgg atacactttc agagtatgcg tttgccggat tgctgcgcgg taccaagacc
    22501 gaagtggtga agtgtatctc caatgacctt gaagtgcccg ccagtgcgga gattgtgctg
    22561 gaagggtata tcgaacaagg tgaaacggcg ccggaagggc cgtatggcga ccacaccggt
    22621 tactataacg aagtcgatag tttcccggta tttaccgtga cgcatattac ccagcgtgaa
    22681 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    22741 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    22801 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    22861 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    22921 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    22981 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    23041 cctattgatt atctggattt tgcctcgcct gtctccgggc tgggttcaaa aatggggctg
    23101 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    23161 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    23221 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    23281 atgacaacct taagctgtaa agtgacctcg gtagaagcta tcacggatac cgtatatcgt
    23341 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    23401 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    23461 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    23521 atcctcaaag atcatcaaat cgtggtcgat attccccacg gagaagcgtg gctgcgcgat
    23581 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    23641 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    23701 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcaccct
    23761 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    23821 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    23881 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    23941 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    24001 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    24061 catccggcaa tcggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    24121 tggtgcagga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    24181 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    24241 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacag
    24301 cccagcggat gacccagcgc gatcgcacca ccgttgaggt tgatcttctc gtcaatctgc
    24361 tcaatcagtc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcgttcatc
    24421 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    24481 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    24541 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    24601 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    24661 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    24721 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    24781 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    24841 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgctgt taagcccatc
    24901 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    24961 atcggcacat ggcccatatg ctccacgccg ccaaccagac atgcctgcgc atcgccggtc
    25021 ataatcatcc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    25081 gtaaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatgtta
    25141 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    25201 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
    25261 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    25321 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    25381 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    25441 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    25501 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    25561 accagcgcca tatccgcttc cgccggagtg gcgataatgc cttcctccag acagcgcacc
    25621 acttcgttga ccatcgggat catcatgcgg gcgataatct cctcttcgct gaaatcgcgc
    25681 ttcggctggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    25741 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    25801 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatcct ctgcgggaag
    25861 cctgctgcca tgacagcctg ggcatgatgc gcggtgtcaa tgcccacgac atccagcaga
    25921 tatgccggac ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    25981 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    26041 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    26101 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    26161 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgtttcagc
    26221 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    26281 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    26341 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggattgtg
    26401 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
    26461 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    26521 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
    26581 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    26641 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
    26701 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
    26761 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
    26821 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
    26881 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
    26941 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
    27001 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtctgcg
    27061 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    27121 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    27181 tcgcagccac cgcccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    27241 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    27301 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    27361 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    27421 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    27481 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    27541 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcgaagtgt gtattttgtg
    27601 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    27661 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    27721 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
    27781 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
    27841 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    27901 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
    27961 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
    28021 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    28081 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    28141 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
    28201 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    28261 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
    28321 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    28381 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    28441 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcc gctgacctga
    28501 cccgtacctg gtcggcaaaa agtgacaacg attacgcaca gctggtgaaa gacgtaaatg
    28561 atgaacaact ggcgctgatc gcgaccatga aagctggcgt tagctatgtg gattaccaca
    28621 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    28681 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtatcggcc
    28741 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacgc
    28801 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
    28861 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctactggca ccgtggcgtg
    28921 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    28981 gcattcgtat cgaagacaac gtggtgatcc acgaaaataa cgtggaaaac atgacccggg
    29041 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    29101 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    29161 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    29221 gtgggtggcg ggtgcgccgg atgattcaca acagctgggt ttctctgacg acggggagcc
    29281 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg ttggggaaat
    29341 taccgctgtg gtggtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    29401 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    29461 attaaccgaa tatactttgc aatgtgaata tcatcagtta accggcattg aagcgttgct
    29521 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    29581 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    29641 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    29701 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    29761 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    29821 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    29881 cgcaaagaga aaggcgaact taaatcccgt gaggggtttc tgatagtggt gctgttctgg
    29941 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    30001 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    30061 gtggggctgg attcgctccc tcacgccatc ctcttttatc gccagatgct gcaatggttt
    30121 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    30181 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    30241 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    30301 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    30361 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga tagcccgact
    30421 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    30481 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    30541 atcggcgtac agtttacgct ggtggttatt tgtaccctcg tactgtggtt tcataatgtc
    30601 tacagttcgg cgctgatgac aattaaccag gcgtttttcc tggtggtgtc gatggcgaca
    30661 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    30721 ttatgttcgg cttttatcgg cggttgtgcc gggtcaacgg gcggtggcct gaaagtgatc
    30781 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    30841 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    30901 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    30961 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    31021 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    31081 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
    31141 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    31201 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    31261 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
    31321 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    31381 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    31441 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    31501 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    31561 gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca ggcggtgaaa
    31621 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    31681 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc acaaagaa
//
LOCUS       DAZFRJ010000038        30997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000038 DAZFRJ010000000
VERSION     DAZFRJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.038"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUN_001550"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUN_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO6659836.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHKUN_001551"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHKUN_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO6659837.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHKUN_001552"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHKUN_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO6659838.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKUN_001553"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKUN_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO6659839.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKUN_001554"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKUN_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO6659840.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKUN_001555"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKUN_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO6659841.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKUN_001556"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKUN_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO6659842.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKUN_001557"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKUN_001557"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO6659843.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKUN_001558"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKUN_001558"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO6659844.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKUN_001559"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKUN_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO6659845.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKUN_001560"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKUN_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO6659846.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKUN_001561"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKUN_001561"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO6659847.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKUN_001562"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKUN_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO6659848.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKUN_001563"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKUN_001563"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO6659849.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKUN_001564"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKUN_001564"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO6659850.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKUN_001565"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKUN_001565"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO6659851.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKUN_001566"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKUN_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO6659852.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKUN_001567"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKUN_001567"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO6659853.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKUN_001568"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKUN_001568"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO6659854.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKUN_001569"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKUN_001569"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO6659855.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKUN_001570"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKUN_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO6659856.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKUN_001571"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKUN_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO6659857.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKUN_001572"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKUN_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO6659858.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKUN_001573"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKUN_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO6659859.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKUN_001574"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKUN_001574"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO6659860.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKUN_001575"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKUN_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO6659861.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAEMPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKUN_001576"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKUN_001576"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO6659862.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKUN_001577"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKUN_001577"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO6659863.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7302 a         8041 c         8483 g         7171 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcat tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gaggggggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aatgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccacattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZFRJ010000039        30947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000039 DAZFRJ010000000
VERSION     DAZFRJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.039"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..208)
                     /locus_tag="ACHKUN_001578"
     CDS             complement(<1..208)
                     /locus_tag="ACHKUN_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659864.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVC"
     gene            468..1289
                     /locus_tag="ACHKUN_001579"
     CDS             468..1289
                     /locus_tag="ACHKUN_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO6659865.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVRRLLCRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISDSDNVDIILWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            1378..2580
                     /locus_tag="ACHKUN_001580"
     CDS             1378..2580
                     /locus_tag="ACHKUN_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6659866.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLIPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            2939..3391
                     /locus_tag="ACHKUN_001581"
     CDS             2939..3391
                     /locus_tag="ACHKUN_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO6659867.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            3388..4224
                     /locus_tag="ACHKUN_001582"
     CDS             3388..4224
                     /locus_tag="ACHKUN_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO6659868.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            4208..5356
                     /gene="traJ"
                     /locus_tag="ACHKUN_001583"
     CDS             4208..5356
                     /gene="traJ"
                     /locus_tag="ACHKUN_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO6659869.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            5353..5643
                     /locus_tag="ACHKUN_001584"
     CDS             5353..5643
                     /locus_tag="ACHKUN_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6659870.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            5707..9717
                     /locus_tag="ACHKUN_001585"
     CDS             5707..9717
                     /locus_tag="ACHKUN_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO6659871.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYKAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQARYARSYINQLPQATEHEYLTRKGILAAPGVRLNNKNELVIPFS
                     NSRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDAGNMIDVAENARQIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQDPNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSATTALRSNDS
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWSAFAEDLNADAPGNATVHNTDNAET
                     NTVSESAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHSSDITDDNRNAPAEELNA
                     DATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLQEERLVSATETSTANEAAR
                     PETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEEVAPPSPPETSPEDTSSA
                     EPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSVDGEPAFLDRLYCLEMVD
                     GASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPAQRAAL
                     EKLRQEKGMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVSPKAEAEQTAGSTAETPH
                     PEAPVTAKAEAPRADRPEQPAASSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESVTA
                     VLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGDPVTLTLQARET
                     FRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFPMATFSQVMLQMQQHWPQ
                     IMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPDELIPAIASADKDNRQL
                     NLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVNHD
                     ENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKAED
                     RQEKTLQQAQSHSPGL"
     gene            9734..10084
                     /locus_tag="ACHKUN_001586"
     CDS             9734..10084
                     /locus_tag="ACHKUN_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6659872.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            10096..10791
                     /locus_tag="ACHKUN_001587"
     CDS             10096..10791
                     /locus_tag="ACHKUN_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO6659873.1"
                     /translation="MSETHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            10802..11776
                     /locus_tag="ACHKUN_001588"
     CDS             10802..11776
                     /locus_tag="ACHKUN_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO6659874.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQTPGST
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            11780..13111
                     /gene="traO"
                     /locus_tag="ACHKUN_001589"
     CDS             11780..13111
                     /gene="traO"
                     /locus_tag="ACHKUN_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO6659875.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGNGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            13108..13821
                     /gene="traP"
                     /locus_tag="ACHKUN_001590"
     CDS             13108..13821
                     /gene="traP"
                     /locus_tag="ACHKUN_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO6659876.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDKRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            13818..14348
                     /gene="traQ"
                     /locus_tag="ACHKUN_001591"
     CDS             13818..14348
                     /gene="traQ"
                     /locus_tag="ACHKUN_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO6659877.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            14395..14793
                     /locus_tag="ACHKUN_001592"
     CDS             14395..14793
                     /locus_tag="ACHKUN_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO6659878.1"
                     /translation="MSRFRAMAARLCAAFIYVQLYLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            14850..15101
                     /locus_tag="ACHKUN_001593"
     CDS             14850..15101
                     /locus_tag="ACHKUN_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO6659879.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            15016..15834
                     /locus_tag="ACHKUN_001594"
     CDS             15016..15834
                     /locus_tag="ACHKUN_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO6659880.1"
                     /translation="MNKTPPLIAPVRERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRQMLREKMDGMALILLPDSFPDKKYVM"
     gene            15831..16250
                     /locus_tag="ACHKUN_001595"
     CDS             15831..16250
                     /locus_tag="ACHKUN_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659881.1"
                     /translation="MTHTGAGDVLPALSTYRGTKMDTLSAAYQFTADAFRHFIIRTLT
                     DDIPPDGVEKEIARFILRHPAFNGGWLLFLAAEIHEKSRHHDCITVTVSVDKYLSWMD
                     IAVSGLPELPDMPFCDFFIRRQQFIYPGVPPTKSGEE"
     gene            16250..16630
                     /locus_tag="ACHKUN_001596"
     CDS             16250..16630
                     /locus_tag="ACHKUN_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659882.1"
                     /translation="MKIHRISPETLITLIRAHLAGKAESTTTEEHRLLRRFLRDDDGR
                     LAGVLLNIAGILQFNRALSALHNHPAAPLAEFSLRKRRKQLHLCLCSLRFFYVPPVFI
                     QNKRRKSIVVHINKITYPPVNSLH"
     gene            16806..19850
                     /locus_tag="ACHKUN_001597"
     CDS             16806..19850
                     /locus_tag="ACHKUN_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6659883.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNTGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            19850..20467
                     /locus_tag="ACHKUN_001598"
     CDS             19850..20467
                     /locus_tag="ACHKUN_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO6659884.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            20428..21633
                     /gene="traW"
                     /locus_tag="ACHKUN_001599"
     CDS             20428..21633
                     /gene="traW"
                     /locus_tag="ACHKUN_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO6659885.1"
                     /translation="MQRQPATTLLALLIGCTVIPSTHAYPVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            21630..22199
                     /gene="traX"
                     /locus_tag="ACHKUN_001600"
     CDS             21630..22199
                     /gene="traX"
                     /locus_tag="ACHKUN_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO6659886.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            22272..24431
                     /locus_tag="ACHKUN_001601"
     CDS             22272..24431
                     /locus_tag="ACHKUN_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO6659887.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSTYTPALGTLKSANEYNSAGSNDPQSAILSSIPGVELTNNVATIFTKTGTTTD
                     QLNPLIKMKNIGDYTMGITESIWAGYTTVRVLSTAGSNSIFGRLVNTVTGLPEAAKAF
                     MEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWAAT
                     HLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIIAAGTILNSLFGAALANV
                     QTDSLTGLFSMAGFLLIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNMVS
                     SVKDIIAGGNTNMRRTPGVREEKLKNINRDDKDGIKQ"
     gene            24519..25166
                     /gene="excA"
                     /locus_tag="ACHKUN_001602"
     CDS             24519..25166
                     /gene="excA"
                     /locus_tag="ACHKUN_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO6659888.1"
                     /translation="MSTTKNRYPSWWAYYYLIRNAYFIIGIPFLLIYAILGTLSISYA
                     RYSSPNIYMITYGCSTLIILPLLWLYLKTRAKKKKVLQVVQQIKESSPFAPTSDYEQL
                     SFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSLP
                     MIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(25480..25632)
                     /locus_tag="ACHKUN_001603"
     CDS             complement(25480..25632)
                     /locus_tag="ACHKUN_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6659889.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWIVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            25965..26207
                     /locus_tag="ACHKUN_001604"
     CDS             25965..26207
                     /locus_tag="ACHKUN_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659890.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(26300..27835)
                     /locus_tag="ACHKUN_001605"
     CDS             complement(26300..27835)
                     /locus_tag="ACHKUN_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6659891.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNLNEAGEFITGIPVLYFIVSLIIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKHNQKDSWDIVNVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGRHDTLISVIAANADKKYFLKEGSSSSRRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHPNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVFVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(28035..28490)
                     /locus_tag="ACHKUN_001606"
     CDS             complement(28035..28490)
                     /locus_tag="ACHKUN_001606"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFO6659892.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAALCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMTTGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(28578..29201)
                     /locus_tag="ACHKUN_001607"
     CDS             complement(28578..29201)
                     /locus_tag="ACHKUN_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6659893.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            29366..29833
                     /locus_tag="ACHKUN_001608"
     CDS             29366..29833
                     /locus_tag="ACHKUN_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6659894.1"
                     /translation="MIKYMFSLAIIFSGSLCAAQIQGNVIRVLDGDTLEILQDKKTVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            29984..30160
                     /locus_tag="ACHKUN_001609"
     CDS             29984..30160
                     /locus_tag="ACHKUN_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6659895.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(30223..30318)
                     /gene="dqlB"
                     /locus_tag="ACHKUN_001610"
     CDS             complement(30223..30318)
                     /gene="dqlB"
                     /locus_tag="ACHKUN_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO6659896.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELVD"
BASE COUNT         7942 a         8675 c         7612 g         6718 t
ORIGIN      
        1 aacaaactat ataaaccagc caaaacagaa tgactgcaaa agaaacacac acggccccca
       61 taaaaataaa tgtcatcgaa tcccatggtt tcaccagtac aaccggaacc atggctgcca
      121 gaacaataag agcagcatta cccaacagcg tctctcgaat aatatccctt ataatctcca
      181 gtttttcttt atgctgttta attttcatat gaacctcatt gatttcatcc cgaaaaggaa
      241 aagattatat aacgaaatac tttcaatctc ctgactccgt ctgctctgca gcataatgcc
      301 agaccagcgg catgctaaat cacgtcctgt ccgaatcctt ttcattcacg cttaaaacag
      361 agttaaaagc actcttgaaa cacactcgat cataacgatc gaaataactc gtttccgttc
      421 agtaaacacg ctctttagac ttaacatatg aaccgcacgg aggtgaaatg aaacccaaca
      481 gcacaggcat tgctgcccgc atgatgctct ccattgacag ggcccggatt agcgaagctc
      541 tgctcaataa ccgccagttg cagtccactc ccctgcagat ccgttatccg cagggcgtca
      601 gagaagcact cggaataatg agcgagcaac tctccctttc tgtatccgac ctgacgcgta
      661 tcctggtcga agatgcatta actgaaatgt ttctcccggc tgaaaacatc gttcgccgtc
      721 ttctctgccg gacagaacac atcatgcagg cacatgatgt ctgcgtaaca acgatgacaa
      781 cgttactggc tccctggaat atccgtcctg ccgtcttccg tgacccggac aaactcgcag
      841 attatctgac aggagagatt ctgacggctc tggctgactg gttttatctc aacccggaat
      901 ggctgaacgg cagggtacgt tatcctttaa gccgtgccgg tgagtggcca gccacacagg
      961 atgcgttctg ccagcttatt tcagacagcg ataatgtgga tattattctc tggcacagtt
     1021 ttccttttgc cggtgttcat tcacaggaat attgcggtgt actgttccgc aagaaaaaag
     1081 aaattaataa agccatcatt taccctgtac tttctttatc gccagtaaga atgaataagg
     1141 aaaaagaaaa ctggtttcac actgcccgca atttatatgc aaagacatcc gtccggaccg
     1201 taacgcttcg tccggcacag gcagaatatc tgatgacagg caaaatatta cctgctgagt
     1261 tattcagtat accgttatta ccctggtaat aacgcagaga aaaaccaata aatttttaat
     1321 ggcaccgtcg tgtcataccg ttgctgtacc caaaacacct caaaccagga aactgaaatg
     1381 atgatgaata tgcacattac ccgaactctc atcgcatctg ctgttttatt ttcctttaat
     1441 tcaacagccg caacttcata ttttgaagcc cgcaatgatg caatgggggg aaccggggta
     1501 gcctcctctc actacggtgt ggcacctctg gcaaacccgg ccctgctgac aaaagccggt
     1561 ccggaagatg acttcagtct tctgattccg tccgttggtg ggcaattgtc cgatccgggt
     1621 aatatcagtg acaacgcaga caaaatcagt gatgactgga aagcctttga tcgtgccatc
     1681 gacagtcaga gcaacgtgtc gcagactgct gcacgtctga agacgcgact tcaggacttc
     1741 cgtcacacgc atgctgacgc acaggtggga acatctgctg tggcgactct tcccggtgac
     1801 accctcgctg ccgctctgat gctgaaaagc tacggtacgg tcagtgtcga cggtaaagtc
     1861 agtgatgccg acctgaacta tctggaaagc attgcaaaca gtaacggtca ggatgtggat
     1921 aaagacagac tgacctctca ggcttttgcc cgtgccgcac tggtcaccga tgtgggtatt
     1981 gctctggcta ctgaactgga aacggccgga caaaagtggt ctctgggctt cacaccaaaa
     2041 ttccagcgtg ttgacctgtt taactacaac gtgctggtta aaaattacga cagtagcgct
     2101 ttcaaaggcg accgttacca caacaccaga aacggcatta acgctgatat cggggccagc
     2161 atggacctgg atgacaactg gacactgggt ctggtcgcac aaaacctgct cccccgcagt
     2221 attgagacaa aagaagtgaa cggtgtcact gaaactttca ggatccgccc gcaggtgact
     2281 gccggtgtct cctggcacaa cgacatgttc actacagcac tggatgttga cctgaccccg
     2341 gccagtggct tcacatcaga cagtaaacgt cagtttgctg ccattggtgc tgaatttaat
     2401 gcctggaaat gggcacagtt acgtgcaggc taccgccaga atctggccag caatgatggc
     2461 agtgcgttca ccgccgggct gggtatctca ccatttgatg ttgtccatat tgatgtggcg
     2521 ggtatagcag gcacagacaa caactacggt gccgttgcac aactgcagtt cacattctga
     2581 tcatctctgc cccggtactg accggggtat ccagacctgc atcctcgcgt tttccgacca
     2641 tcaaacgaca cagccagacc tgtagtgctg cctctgatct ggctgttatc tgacatatca
     2701 ttttcgcatg aggaaatgca tgtttaacaa aaataaaacg catatcgaaa acaaagcctt
     2761 tatggtggaa ccgttccggg atccgacagc taacgaatgt aatgtcgtga taacaaacga
     2821 tatttgtatc cgggtagcac tgtcaccctc tgaggtgctc cagccattca gcaaaaacac
     2881 gaaccagtaa atcctcccca caacgctttc ttctttcact gctcaggaaa aatacaccat
     2941 gaaccgtatg atacctgcgg gatgtctcgc cgtactctgc ctgaacggct gccatctccc
     3001 cccggataat acagctccga ccagaaagta tggtgctgcc tctgatgtcg ccgtcgcccg
     3061 gcatgcgcag tatcagctat gggggcaggg aatttactct gcagcccttc cggagcgtca
     3121 gtccggcggc agtctgaagg ctcacagcga ccgtgtttcc tttgactggg aaggcgatgc
     3181 actggaattg ctcaacgaac tggcccgtct gcgcggccag cagttcagct acagcggcgt
     3241 ccgtctcccc ttacccgtct cactgcacgt ccgcgacatg acctttgctg atgtccttcg
     3301 tctcactgaa gcccagactg cctggcgggc aacactctcg cagtcagccg ggcgtttaca
     3361 tctgtctttt atgacgccgg agaaaaaatg aaaacacacc tgtgtctcct gctgtctgct
     3421 ctcctgctta caggctgtgc cggacagcat caccgttctt ctgccgtcat gacagaaaac
     3481 gcaccgcccc cggatatccg tgcatggctg acgccctccg gaaaccagga tgatggtatt
     3541 tcagataccc gtcgtcagat gctcacggat gccggcagaa cgctgggatt ccgtggagga
     3601 aaagcacagc gctcatggga actgattcag gcactgaatg cccgggaaag taccctgaat
     3661 gccctgtatg atttccggcc tctcatcagc aaggaaggct ggctgcctcc tgttatcgat
     3721 gaagcgcagg atgttgcaca catcagaccg gaccagataa gaacagcctc ccgtgtctgg
     3781 accatcctcc gcccggaacg ctttgtcagt aatccacctg gatggcgtga ctggctgctg
     3841 aaaggactct caaccaccgc cacaccaggc actgaaggcc gggttgtgcc ggaagaccgg
     3901 gcacaacgca gactatggga aaacgcctta cgtcagggat ggcaggaagg gcgcctcaac
     3961 gcagatctca cactggaagc taatcagaag cgcctgaccc gtgattaccg gggcatgatg
     4021 ctttacgccc ttctgtggcg tcagggcatg atcacacgtc cagatgtcac agagcagatg
     4081 cagacggtta ccggcaacgg gcaaaaactc atcactggcg atcgcgtccg ccgcctgaaa
     4141 acacatgctg aatttacgct gcagaaatcg cgctggcgcc ctcttgtcag caccgaagga
     4201 gagaaaaatg atatcaccag atgacttcgg taccttcccc ttctcccgtt ttacggcaga
     4261 agagtttcgc catttttttg cctggtgtgc ccgccaccgc gtcagtgata ttgatctgac
     4321 cggcggttca ccggtatccg tcagtcgctt tggacggcgg gttcgctcat ccgtaaatcc
     4381 ccttccgtcc tccatcatga tggcactgag tgatgagctg tttgggcggg aggttcttcc
     4441 ccgggttctg tcaggtcatc ccgtggatcg taccattcag gtcagcggag atgcctccgg
     4501 tcgctatggc ctgcaacgag gtcagcgtgt tcgcctgcgc tgccacctga tacagggaac
     4561 atcggcaaca gaagaaaaag ccatttccgt caccatgcgg gtgatcccgt cagatattcc
     4621 ggacatcctc actatgaata ttgaaccgga tctgctggat gccatgctca gaaaaaccgg
     4681 cctgggcttc atctgcggag aaaccggctc cgggaaatcc acactggcgg cggcactgta
     4741 tcgccacatt ctgaaaaatt tccccgatcg caaaatagtg acctatgaag atccggtgga
     4801 atacattctg ggcggagaga atgaccttct gcctccccat caggccgaaa tcgggcgcga
     4861 tgtggaaagt tttgctgcag gacttcgctc tgccgttcgc cgtaatccgg acatcattgg
     4921 tatcggtgaa atcagggatt atgaaacagc agatgccgca gtccaggcgg gtaataccgg
     4981 acacttttgt atcggcacca tgcacacaaa atcacccgga gaaacctttg cccgcctgct
     5041 gggattgttt cctcctgaaa tcagggattc gatggctgca gccacgcttt ccctcgtaca
     5101 gttcattctg gtgcaggtac tggtcagaac aaatgacggc ggcaggcagg ctgtccggga
     5161 atacatcgtc atgactgacg aactgcgcaa tacgctctcc ggacaatccc atgcaacatg
     5221 gggacattac attgacgaaa tcattcgtca ggaaaaacgg cggataaggg atcaggttct
     5281 gaccatgtat cagaacgacc gcattgagaa atcagaagca gcgctcttca tcccggccgg
     5341 agagttcccc cgatgaaacg cgaaggcttc tggcgggatg taacaccacc gctcaacatt
     5401 tatggtgtcc ccctgccgtt taccatgatt tacctgctgt tgtttccgta tccgtcaaaa
     5461 acgacattct gggtgtgcag cggcattctg ctgtttttta tgctcctggg atattccggc
     5521 tggacagtcc gcaccctctg tatacgtctg tacagcctgc ttcagggtaa gcaggcatcg
     5581 ggacgtccct ggtggtaccg acaccgcaca gaattacccc gtgaatggca cggaccagaa
     5641 tagccctctt ttgtccatca tcccggcgta tcacaacgcc ctcatattta atccccggag
     5701 aacgacatga agtccagata tcgtacctgg cttgctgtcc cgcctgagga gacagaagcc
     5761 gtaaaaaatg cagtcccccc gttaaatggc cgcaaagctg tcgcatggga tccagaaaaa
     5821 aaactctggt atgcccgggc gggtaccgaa ctttcactgc tggagcgctg gttaccccgg
     5881 ccgcaggaac tgtcgatgga tgccggcgat ccggtcaccg aatttgctca ggtcctggaa
     5941 aacgccgggc tggttattca gggattgcca cagatggatg gcgccataca cagagtcgcc
     6001 acccgggatg acaaaaaagg ggccaaaagt ggcgcataca aggcctatct tgatgggcgc
     6061 cccgccggct ggtaccgcga ctaccggagc gccgatgaca gtcccacaaa ctgggtattt
     6121 tccggcggag aacaacacga tcctctggcc agactgcatc tgcgggcatt cgctcagcaa
     6181 cagcgcgatg ataacgcacg taaactgcag caacagtaca acaaacaggc ccggtatgcc
     6241 cgctcctata ttaaccagtt accacaggca acagagcatg aatacctgac ccgaaaaggg
     6301 atccttgccg caccgggcgt ccgtctgaat aacaagaatg aactcgtcat ccctttcagt
     6361 aacagccggg gagaaatacg ttcataccag cgtattcctg tcaccggcgg gaaggatgcc
     6421 cgcattctga aagactcaga gaaaaccggt aactggttca ccttcggaac cccggaaaat
     6481 ggccgtcccc tgttatttgc ggaaggttac gcaactgcag cctccctgca cgaagccacc
     6541 ggccttccgg ttctgatgac agttgatgcc ggcaatatga ttgacgtggc agaaaatgcc
     6601 cgccaaatct ggactgacag cccctttgtc ttctgtgccg acaacgacca tcaacggaaa
     6661 attaataaag gtgtttcaag tgcaaaaaaa gctgccgcaa gcacgcatgg cgaagtcatt
     6721 attccggcct ttacggaggc ggaaaaagca cagggactga ccgatttcaa tgacctggac
     6781 gccagcaggg gacgagacgc ctttcagcga gccatgaacg cacagttaga atatatcggt
     6841 atccccacgc ccaacaatga cactactgac cgccgtgaag ctatcgtgat cggcaacctg
     6901 atattcacac cggtaaaaga tgagaagcca caagtgtccc ctgaaaacag acagagcaca
     6961 gcccctgaac cggcagtgtc ggaacagaat catccttatg acatccctgc agaagagaca
     7021 gacaatcagg catatgaaga tgactatgca gcatatgcat ccctgcagtc ggtaactgca
     7081 gggacagcct ccccgaatga accgcaggtg aatgctgttt ctgccattga cagcaacgca
     7141 aagaatacct ctgccgccac cactgacgac cggaatgtat tagcacaaga ccccaatgct
     7201 gacgccacgg ttacgactac ggctcacgac accggcacag cagaaacaga ttcaccctct
     7261 gaacctgcag agattcagcc agtgcagcag gccagtcctg aaccatcggc taaagataaa
     7321 ccacaggaga acgtagtgtc tgctactgat aacgacgcag agaacacctc ttccgccacc
     7381 actgccctcc ggagtaacga ctcaccggaa acatccgtct ccgacgcaac cgggacacag
     7441 cacaccgcca caacgcaacc tgaagaacca cagcaggtct ccatgggcag cgctgacgac
     7501 tggagcgcat ttgcggaaga cctcaatgct gatgctccgg ggaatgccac tgttcacaac
     7561 accgacaacg cggagacaaa cacagtttct gaatcggccg ggatgcggcc agttcagcag
     7621 gtgaccactg aactgacggc agaagataaa gcacaggaaa agagcgttgc gtctgccgct
     7681 gagaccggcg cagagcactc ctctgacatc accgatgaca acaggaacgc acccgctgag
     7741 gagctcaatg ccgatgccac ggttgctgtt cacaacaccg gcacaacaga aacagcctca
     7801 ccttctgaac ctgcagaaac tcagccagtc cggcaggcca ccgctgaacc gacggctgaa
     7861 gataaactgc aggaagagcg tcttgtgtcc gccactgaga ccagcacagc gaatgaagca
     7921 gcccgccctg aaacgccatc gcttttcagc cgtctgcgcc gttttctgac cggtgtgaaa
     7981 tcacgcacct cagaaccttc cgttattcag gagaaaaagc ccacggaggc tgaaacagca
     8041 acagaggagg tcgcaccacc atcaccacct gaaaccagcc cggaagacac atcgtcagcg
     8101 gaaccggaca cgattatctt tgcaccccgc cgtcccgaca gtccgcagcg gcacaacctg
     8161 gacgagatcc ttaaaaatct tacacacaaa gagtttcccg acaggactgc gctctacagc
     8221 gttgacggtg aacccgcctt cctagacaga ctctactgtc tcgaaatggt ggatggcgcc
     8281 agcgcggatg acaaaaaggt cctggctgcc cttgctgtgg ccaccaactt ctatggcggc
     8341 gttatcgagc tgaccggcag tgatgccttt aaacaaaaag ccatgcagct aattgtcgaa
     8401 tatgacatca aggttcgcat gaaactcccg gcccagaggg ctgcgctgga aaaactccgc
     8461 caggagaaag ggatggcgca tgatgccatt gtcacccaca gacccacgcc ggaactcaac
     8521 cgccagtcac cggaagagac aacaatggcc caaccggctc cccctgtgtc accgaaggca
     8581 gaggctgaac aaacagcagg cagtacggct gaaacaccgc atccggaagc gccggtcacg
     8641 gcaaaagctg aagccccccg ggccgacaga ccggaacaac ccgccgcctc gtcgtctgcg
     8701 ccagaaaaag cccctgtcag tgcacagccg cagcaacccg acacaccgga ggaagagccg
     8761 cccggcaagc tcagacccgg tgaaagtgtg actgccgtcc tgacaaacta cggagaaaaa
     8821 gagtatgcgc ccggaaaagg gcgctgcttt tttgtggagc tcaccaaccg cagtggcaaa
     8881 cgggaatact ggggaaacgg cctggaagaa ctggtcaaaa accatcagaa gggtgacccc
     8941 gtcacactga cactgcaggc gagagaaaca ttccggctgt ccggtgaaac gcgggatcgt
     9001 atccgtaata tctgggccat aacaccggtc agtaacggca ttaccgttaa taatgaccaa
     9061 cctcatgcgg gtcagcatac cctggagttt ccgatggcaa cattcagcca ggtgatgtta
     9121 cagatgcaac aacactggcc acagataatg caggaagttc gctcccccaa aaatcctccc
     9181 aataatctgt acattggtga agacaggcaa cctgcatcag cacctcagga tcaatcaatg
     9241 cgactccccc tcgctggtaa tgcccccgat gaactgatac cagccatcgc ctctgccgat
     9301 aaggacaatc gtcagttgaa tcttctgctg gtccacagcg cagatgatca tctgcagggc
     9361 gtggtccgcc tgaacggcac cctttacccg gcactggcca ccccgtcagc tgacaaccgt
     9421 cagctggtca tcaatgccct gacggataac ggtctgcagt ttgccggtta cggtgaagct
     9481 gttaaccatg atgagaatac tcatcaacgt ccgtccccac aaataatgca gtttcacctg
     9541 aagcaacagg actccccgtt gtttgccgcc atccacaaac cggaagagca accggacaag
     9601 cttttccgct ctctgggttt tgagcagacc tggaaagagt ggtctgacag ccaaaaggcg
     9661 gaggacaggc aggaaaaaac tctgcaacag gcacaatccc acagccccgg cctgtgagcc
     9721 ggtaaggacg accatgaaca tgaaaaaaac gctgctggtc atactgacca gcatctcgct
     9781 ggcttctccg gctgccgtgg cagcaacctg tcgggcaggt gctgccgcag aagccggcag
     9841 caaagccgcc tgggagcggg cgcgtacagc cagccaggca tggtcacagc gagaaaacga
     9901 cgtcgcctcc tccctgcagt cctgcctgtc ccgcattaag gacgtcagca tcactcttcc
     9961 acaattcccc agcctggaca aaatccttcg cgatctggaa aaccaggtct gtgatgccgt
    10021 cgtggatcgg gtgaatgaac atctgccggg gaatatcgac ccctggaaag actataacct
    10081 ctgaacagga gccccatgag cgaaacacac cccacatccc ctgtctctcc ggaaacagat
    10141 aacagcggag atatttataa acaaacgctc agtaccattg cacagtcaga acaacgggcc
    10201 gcactggttc ccggactggt cagagccttg ctgtgtagca gcgcagctct cgtcatcagt
    10261 ctgaccggca atatcataca gtactggcat aacagcaacg taacgcgtga atactttgcc
    10321 accgacaatg gccggctggt ccgcctggcc cccaccagtc agccagcctg gagtcagagc
    10381 gacgtcatga cgtttggcag catgacgctg agcgaagctt ttaatctgga ttttgtccac
    10441 tacaggaaac aaatcacgac actggccccc cgcttttccg aagccggttt tgccggctat
    10501 gtgaatgctc ttcaggcctc caacattctg gacaccatta aaaaggagaa atttaacctc
    10561 acagccacca tcggggccgg cacgctggtg aatcagggcc agatggaaaa cggcatctgg
    10621 ttctggaaat tccagtatcc ggtcaggatg cgtctggtcg gtcagaccag cacccgcccc
    10681 gagcaggcct tcacctttga aatcactgtc cggcgcgttg acccacgaat caaacccggc
    10741 ggcatggaaa tcagccagat ggtttcccgt aacgccgcat caggaacctg agactcatat
    10801 catgcaaata catcatcttc ttggcgcact gcttattgtg tgcggcactg tcacggctgc
    10861 agataacccg ggctggcaga acgcacgaac cccacaggct ggcggaaaga caggacagac
    10921 tcccgggagc acccccgcag gcggcccccc gccagccaca caactcacag gccccctgcc
    10981 gccacctgcc tctcctcttg ttcaggaagc cgcatcactg gattcccctc tgaatgcagg
    11041 tgaaattcgg gaactgcggg gacgaatggc cgataacgaa cgggccatca gcgcacctgt
    11101 caccagtgtg gtgccccgta tcagctccct cacggtaaat ctgtcacccg gggccagtct
    11161 gccgcttgtc cgaaccgcac tgcataacgt cagcgtggta acgttcacgg acatcaacgg
    11221 ctccccgtgg cctcagtcgg atccgcctta taacgcggca cccggtcact ttgatgtgca
    11281 gttcaacggc aatatggtct ccgtcactcc cctgcgcgcc tgggcggcag ggaatgtaac
    11341 ggtctacctc aaaggactgt ctgtaccggt cattctgaat atcaccagtg gtgaaaccga
    11401 caccaatacc ctgagtcagg agatcgacag ccgtctggat ctgcgcattc cccgtcaggg
    11461 acctgccagt cctgtggtag ccgtaccatc agagaagatt gccctgcatg atgccaccct
    11521 gcaggccttt ctggacggtg ttcctcccga tgatcccacc gtgcgtcggc tgaaattctc
    11581 cggaaatgtt ccgggcacca ccatctggca gcacggcgat gatctgctcg tgcgatcgcg
    11641 ggccatgctg cgggatgaat ttgaacagac gctggcatct gctgacggta cgcgtctgtg
    11701 gaaactgccg gtgactccct tactcacctt ctccgtgaat ggtcagtcca ttcacgtcac
    11761 ccctgaactg gagtaaatca tgagcgcaga acaggacaca gccgcaaagt ccggcaaaaa
    11821 actgtcagcc ctgctggttc tcgggggcgt gattatcatc gggggcggat acctgctgct
    11881 cacctggctt tcccccgaaa acagcgcatc gctctcgtca gtcaatatca acagcacgac
    11941 aacaggcaac gggcggaccg tggtggagtc acagcactac cgggatttac tgcgtgctga
    12001 caatgagcgc ggggccgcag aagcgttacg taataaccag acgttcatcg ccagtctgcc
    12061 caggggactg gatactgatc acggaaataa tcaaagaagg gatactcagc ctgctgacca
    12121 ggccgagggt tctggtcaac catcaacgcc ccctcaggca gatcaaacag cccgggaaaa
    12181 acagcgggaa gcgttacaaa aactgcttgc ccgcatcaac acacagcatc ctgacgcccg
    12241 ccccccggta gtggcggctg caatgtgggg taccactggt gctcccggga taagctcaca
    12301 cgcctcacca cccggtaccg gcacatcacc acaaccacgt ctgcaggagg ccagtctggc
    12361 aaccccggca gctcgtacag ggatacagct gataccggca ctgacccgcg tacctgcctt
    12421 cattgatacg gaagtggact ctgacaatgc tgtctcccgc gtggtggcct ccgtacctgc
    12481 cggcccatgg gccggggccg tcctctacag ccaggacgcc agactcgccg gcaagggaat
    12541 ggatatccac tttgaccgga tgatgtggcg gggcatgttt ctgaaagtca atgcatatgc
    12601 cctgaatgaa cataccggca tgtcatccgt ggccagcgac gtgaaccacc gctggtttaa
    12661 gcatatcgtt ctgccgtctg tgctcggtgg ggtgggcagt gtcggaagtc tttacaaaga
    12721 cgccaacaca caggtcattc agggaaacta cggcagcatt accggcaggg tgggaatgcc
    12781 tgatggcgag gcgctggctg gcgtcattgc aggcggcatg gccgaacggg gcagtcagtt
    12841 gctgacccgc caggcggaga gcgaacctta ccggcaggtg acagtgtcac gcggagaggt
    12901 tgtctccatt ctgtttgttg agccggtcat gagcaacgat atcagtcccc cctcctccgg
    12961 cactgcccct tctgtgccac cgccctccgg acgcccgctc acccaggaag aagcagagcg
    13021 tcaggcgtct gacaggatcc aggcagctgt catgcgtaaa cagaacgaaa tgcggcaacg
    13081 ttacggaata caacaggaaa gtcatccatg aaaaacgaac atgaagatcc cggcagtttt
    13141 ggcgaaacaa cgacaccggc atccggcaaa tccttcctga aacgccagta tatgggatac
    13201 agcctgccag tctgggccgg ctgtgtcctc atcatcctgg ccgcagtctg gtacaagttt
    13261 ctgcggacac cctcagagcc ccctgcagat gcaggcttcg ctgtcacacc gcccgcagcc
    13321 gaaagctggg cagagcccat gcccatgacg gagaccggga gcggaaacac catgtccgcg
    13381 cctcctgtca ccccacaggc agacatgcag cagcttgcac gtgacctgaa agcagagctg
    13441 gacaagcggg atgaggaaat ccgacgtacc ctgaccatgc tgaaagagag catcacaacc
    13501 cttggcgaca ccatacggaa agatgaggcc tgggcacagg aaacccgtca gttgcttgat
    13561 gaaacccgta agcaggtgca tgccctgcag atgcagggac cactgcaaat cccgaccggg
    13621 aataacggtc agtcaacgaa aaccccgcct tcccgcctgc agggcatgca aatcatgtcg
    13681 gtggaaaaag gaatggcctg gatccgctgg cagggtagcg cctgggcagt acgggaaggt
    13741 gacaggctgg ggaatatcac catcacccgg attgaaccgt ccagccgctc agtcatcacc
    13801 tctgccggta cattacgatg atgagcattg atgccctgac agccatagag aactttgcca
    13861 gcggtatatt cagtgcaggc atggagtttc tcttcacctg gggagagctg ctggggatta
    13921 tcggcctgat tggtcatctg atgcgtgccc gggctgaagg gcgtcacagt atgggaccgg
    13981 gaaaatttat cgccggtatc gtcatctgcg gcatgctggt cgccctgcca tccttcatca
    14041 atgccggggg tacccagatg ggattcaggg cagattcctt cggccctatc gcttatgtac
    14101 aaccaaccac ctttggtgcc gctgccggtg ccgccaatgc catgctctca ctggtaaaac
    14161 tggccggagt ggggtttgcc atgaacggca tttccatctg gcgtaaatcc ctgctggacg
    14221 ggcataccgc cctgagcagc agtgagtcca tcagcaaagg aaacgtcaag tttgttgccg
    14281 gcgttctgct ggtttttatc gatcgcgtca tcgatgcctc tctggcgagt cttggtctcg
    14341 ccttctgatc cacattcatt ttcagtcatt cagtctgtta gttaaggaga ctatatgtca
    14401 cgattccgtg ccatggcggc gcgtctgtgc gctgcattta tttatgttca gttatacctg
    14461 gtggcctgcc gtgatcggct gatacgcctg ttacttctgc ttcccgtgat actgctgccc
    14521 cacacagcga tggcaaacac cctattcgat atgatcaaaa ccgttggcga tggtgcaaaa
    14581 ggctccagca gttcatttgt ggccatcggt caggttattg gcctggtgct ctttatcggc
    14641 ggtctgcttg gcctcaaaca aatcggtaaa aaacccggag cagggctggc ctgcctgctg
    14701 tcaattctgg tcggcctctg ccttgccgtc gtaccggaac tgatgggcag cggacagaaa
    14761 acgctcggtc tttctccggt ttcaatcagt taatccgcaa aggcaccggg aagccggtgc
    14821 cgtcttcaca caatgaggag gaccagacca tgtcctgtga gctgccccct ttcaccctgc
    14881 tcagaacaga gcacggcaac gtgattgccc gcgacaaccg ggataatcag gagtttgtgt
    14941 ttgcagagtg ttattccacc gccatcgcag cactgattct ggctgccgtc accctgtata
    15001 cagagagaaa aacgtatgaa caaaacaccg ccactcatcg ccccggtccg ggaacgctgg
    15061 cttttcccgc tcccgcaacc ggacacggta ttcagcggtg aatccctgct gccaccacca
    15121 gtgctctcaa cacccggacg ctgtccctgc tgtcgcagga ccgtcactca ccgcttcatt
    15181 ctggaggatt catggccatt acagcagatg gctgacacct gccgggatac ggtcgtcctg
    15241 ctggaaaaaa acctcacccg ggtgatgcgc ctgaaaaaac atccggtacc ggaaaacgct
    15301 gacgaaaaaa agaaacacac ccgaaccctg caggatgcgg agcggtccct ggcacaggcc
    15361 cgcctgtcgg ccaggcgact ggccctgcgt catgtggaaa agtcacaaat cgtcacaaca
    15421 gatgcactca gcgaaaatga aagtgaactg ctgcagccgg aaggcccgcc ctttcatctc
    15481 tgtgccttct gccacgcctg gcactgcctg aacggctacg ctgccgcaca gggggtgatg
    15541 gtctggctcc ctgatttgca tccggcctcg gtagttgctc tcaatgcacg ggcactgaag
    15601 gaaattttca gcgatgagcg taagcgagtc agacagggac gggccgtact gaatgccctg
    15661 gtacagaacc gcctggccgt tgaggagaaa tttcgcacct ggcgtccggc ggattttgct
    15721 gatgcactgc gccgctggcc gccggcacag cgtcaaatgt tgcgggagaa aatggacgga
    15781 atggcgctta tcctgctgcc ggattcgttt cctgacaaaa agtacgtcat gtgacacaca
    15841 ccggtgccgg tgacgttctg ccggccctct caacttacag aggaacaaaa atggataccc
    15901 tctctgccgc atatcagttc accgctgatg cattccggca ctttattatc cgaacactga
    15961 cggacgatat tccgccagat ggagttgaaa aagagattgc ccggttcatt ctccgccacc
    16021 cggcatttaa cggcggctgg ctgctctttc tggctgcaga aattcatgaa aaatcccgtc
    16081 accatgactg catcaccgtc acagtgagcg tcgataagta tctctcatgg atggatattg
    16141 ccgtgtccgg tctcccggaa ttaccggata tgcccttctg tgattttttc attcgccgcc
    16201 agcagtttat ttatccgggt gtgcccccga caaaatcagg agaagaataa tgaaaattca
    16261 ccgcatatcc ccggagacgc ttataactct catccgcgca cacctggccg gaaaagctga
    16321 aagtaccacc acagaagagc accggctact ccgtcggttc cttcgggatg atgacggcag
    16381 gctggccggc gtactgctga atattgccgg aatacttcag ttcaacaggg cattaagtgc
    16441 actgcataat catcctgctg cgcctctggc tgaattttca ttaaggaaac ggagaaaaca
    16501 acttcatctc tgtctctgca gtctccgttt cttttatgtc ccaccagtat ttatacaaaa
    16561 taaaagaaga aaatctattg ttgtacatat taataaaatc acttatccgc ctgtaaattc
    16621 actacactga cagcgtgttt ccaccttatt acatttttat tattcactgt aatcacacag
    16681 aaatacgcac ctgtccctca acagattatt tattttctct gctgtttttt catgccacat
    16741 cccctgctgt ggcatcaaaa aatatcagac cgtgatgatt tatccttacc ggagtgctcc
    16801 atgccatgaa tatcaaccgc attatttttg ccattgaaga ctgtatcagt accctgtcac
    16861 ggtattccgt ttccgcattc ttccccgatt actgtgatct gcacaccgtc atcggtctgg
    16921 accaacagga ccgcgaacgc cgcccctggc tgaaagcccc ctacattgcc accacaaaac
    16981 aggggtatta cctttccgtc tttgaaatca gcggtgccct gaaagaaatg gatgaaaacc
    17041 cggacctgac agcccccggc acactggaat cactgattac agcgctggcc gagcgaatga
    17101 acactgccta taaaaacacc ggtcacaaaa tcagttttgt tttcgaacgc gatccggagc
    17161 gtggaaggga tgaaatcgaa gccatgctct taccccagcg aaaatccatc gcacgcacgg
    17221 ggatcctgct tcaggacgtt ctggatgaaa aagtcaccac gctcaccccc tggcttgtcc
    17281 gggaacgctg ctggctgacc gtctggagct ctgaagaact gctgagccgg accgatcgca
    17341 aggaccacca gacccgtatc aggaaactcg ctgaacacgc tccgccagcc cgatttgcgc
    17401 aggatccatg gcgctggaca ctttccgcac tcaaaatacg gcatgacgcc tttctggaca
    17461 cgctggagca ggccctgaca catgattctg atggtctgct ggttcgcctg atggatattc
    17521 atgaagtggg acgggaaatc cgccgtcaac tggagcgcaa cagcacacct gcagtatggc
    17581 agcctcatct gcctgaagat gcccgtcctg ccggctggcg acagggcgag gatacctctg
    17641 tattccacgc accgtcgctg aatctgcagt tattcaccac acagcctgaa acacacggca
    17701 gcctgataaa ggccggtgac ctctggcacg gtatggtcgc cattaccctg cccccccaga
    17761 atctgcggac attcaaccag ctggtcaggg atgtgccccg cgcaatcccc tggcgtatcc
    17821 ggatggatct gatgcccggc ggcatgaagg cgcttggggt caagaaaacc ctgctgacct
    17881 acagcagctt tatttcccct ctccggccca tgtatgactc ggtgatgatg ctgaatgaca
    17941 tcaacaaaca tgatccggtc tgtatcatga ccattgttgc caccacatgg ggaaacagcc
    18001 gggaagtctg tacccgtaac caggctctcc tgaaatctgc gctggaaggc tggggggtat
    18061 gcggcaccac caccaccttc ggtgatccga gacgtgcctg ggtgaacacg gtgctggcag
    18121 cctcccacag ctccggcccg atcccccttt acccgcccct ttcacacgcc ctttccctgt
    18181 ttcccctgaa cagggcaggc tctgtctggc ggggacaggg aaatctgatg atgcacaccg
    18241 aggacggctc tgcatgggag gtcgcactgg catcgtcaca acagaacaag cacacggaac
    18301 tgacgcccgg cgcacccggt ctgggcaaat cggtactcat caacgcactg tcggagatcc
    18361 agatagcctc agcccagaag aacctgcctt tcattgccta tatcgacaag ggattcagtg
    18421 cacagggact ggtacagctt atccgtgaca gcctgccgga gcagagaaag gacgaagccg
    18481 tggggatcat tctgagcaat gatccggaac acacccgtaa cctgtttgat gtgatgtacg
    18541 gtgctaaaaa acccgccacc ccggaaaaga acttcatggt atctgtactc tgcgcactgt
    18601 gcgtggatac cggcaccggt cagccctgta atcccggtga cacccggcaa atcataagca
    18661 acctggtgga tctggccttc agggagtatg gcgaaaacaa ccccagactg taccgtgcag
    18721 gaacagagcc ccttgtggat ctcgccctgg aagagagtgg catcgcagaa caacatgacg
    18781 ctggatggtg gaatgctgcc acctggtttg aaatcaggga catgctgcat atggctggca
    18841 atatccccgc agcccagcgg gcacactacc aggccatgcc cctgctggct gaaatgtccg
    18901 ccctgctggg acagccatcc atccgggatg tgttcggcac agttcagcgc gataactcag
    18961 cagaattgtt actggactac atccgccgtg ccctcgatca gggacacagt gactacccga
    19021 tgatgtccgg ctgtacccgg tttatgctca gccccgacac ccgcgtcgtg gccgttgatc
    19081 tgaacaatgt ggccggtgac aagacacctg ccggacgtct gcgcaccggc atcatgtacc
    19141 tgctggcagg acaaatcgcc ggcggggatt ttgtcctgcc gcagtatcag gaggaaatac
    19201 ggaaaaatct cgacccacgc taccatgagg tgattttccg ccgcattgaa cagcttgatc
    19261 aggaggtcaa gaccaaagtc tatgacgaac tgcacaatgc aaaagggatc aatttcatct
    19321 gggaagccct ggacacccag gagctggagc agcgtaagtt cgggatacgc acagtgctga
    19381 gcacccagct actgcaggac tatccaccgg cagttctcaa atccgccaac accctctggt
    19441 tgctgcgtta ccgccctgac gaaattccgt ttctgcggga taattttggt gtccctgaag
    19501 tcaccctgcg gcgctttctg aaaatgccgg aaggggctgc accggatggc agcggtgtcc
    19561 cggtactggc tgtgttccgt gtaaaaaacg gtacgctggc ccggatcctg aaattcactc
    19621 tcggtccact ggagctgtgg gcactgaatt cctcacccaa agacagcgcc ctgagacgtg
    19681 cgctgacgca ggagctcggg tcactgcggg cacgccagat cctggcagaa aactttcccc
    19741 gtggttcagc cacctcactg atagaacacc gcgcccgtac acatgacagt gaaaacgtca
    19801 tccatgatct ggctgcagaa ctcatccgta agcagggata tcacctctga tgtacagcat
    19861 acacatcact ccccgctgcc ggcgccctgc acggggacag gtcttcctga aaacgctgcg
    19921 cgtggacggt gacagtttca gcttcaccct gccgtccgcc tgcttcacgc tgcgtccctc
    19981 cccaaatccc cgggtcattt tcgccagtga cagacgggct gacggggaaa tgagtggcgt
    20041 tcgcctggct ctgcccggag agacaggcac actgataaca acggccacct ggcaggtcaa
    20101 cgtgccggag cggcctcctt ttgatataca gcatgtcatt cactggctcc tgccctcgcc
    20161 tctcacggcg ctgatgtccg acaacacctg gctgtggccg gatagccccg gcacaccggc
    20221 actgacggcg ggagactggc cggtaatgga aaccgatacc ccgacgctga aacgcgtgaa
    20281 gcgtcgtacc ttcacgctca tcgataaccg caaccgtcag ggatggatca cccgtcgctt
    20341 ccagtgcttc ccgctggctc cgctgccgga atcacacagt caccgcgtac cagacatcct
    20401 gtatcccctg aaaacggaga aaaaagaatg caaagacaac cagcaaccac actgcttgca
    20461 ctcctgatag gctgtacggt tatcccgtcc acccacgcct atcctgtgac cgtagtggcc
    20521 agccagcctg tcaccacaca aatcatgccg gagctggccg ccattcaggg cgtgatggca
    20581 gagatggtcc gtatgcagac agccaccggc acagccatca accagagttc agagaagctg
    20641 gcctctgtca tcgcacagga tgggcaggcc acccgtcagc agatgatatt cagtaatgaa
    20701 acccgccggc ttgaggaagc ccggaagagc ttcagcgtgc cggactccat ctgcagtgaa
    20761 tccgcctccg gagttgcagc tgaaagtcgc cgtgcagcag cgtctgcggc tgcacgtctg
    20821 agtcagggag gaggcgtcag cagtaaaccc atccgtgaac gtctctcccg ggcagcagac
    20881 tcaccaaccc gcgaggcata tgacagtgca ggcattcatg ccggttactg cacggaagct
    20941 gaatatgccc gctttggcgg aaccgatgtc tgccctgccg tgggcgagct gccgggcgga
    21001 gacagccagg tgcgatcgct ctatcagggt gcaggcacag cagatacccc tgccgccctc
    21061 acctgggatc agaaacagat tgatgctgcc acggcttaca tgaaaaacac cgccagaccg
    21121 tcagcgggtc gtgccccggg gaaaggtgaa gtgggaactc agaccgggcg cacgtatgtg
    21181 ggcctgcaga atgaatacaa cggcatcatc gatgccgctt ctcatccgca actgtcactg
    21241 atagctgaca gtacccctaa tgaggccacc cgtggagcgc tgactgaagc cctgcagtca
    21301 ccatccgctg cggcatattt tgaccggacc gcatcatcag aggcccgcac ccgtggtcac
    21361 atgagccagc gggagtttga ggcctttgaa gctggcagac gctatgcaaa caccgactgg
    21421 cagcaggatc tgcagggcat ggaaggcgat aaccttctcc gtgaactgct gcgcactacc
    21481 gccctgctga actggcagat gaacgacctg aaagagcaga tacgccaggg taatgttatc
    21541 gccgggcagc agctggcact ggcggcacga cagtattacg gacagcgtct cggtgaactg
    21601 tcacaggcca tgagccaggg gagcgtaaga tgaaagacga caacagcacc gacaaaaaaa
    21661 aaccggcccc ccgtctccgc cgcatcggtg acatcgggat aaacctgttc gcaccactgg
    21721 cagaaacccg gtggatgctg cgcagctgcc gccatatgct gggccagcat aaaacgcgcc
    21781 tgaagcgcct catgcccgac cggcaaccgg agagtccggt ctgtatgacc tgggaagaag
    21841 ccgtcagggc cagtggcctt acggtcaacg aactggatcg gcgtctgcga cgtcgcagga
    21901 tgttctggcg gacaggattc tggctgatga tgataccggt acttctgctg tgcggcatgg
    21961 cccttgctgc cacagcgctt ccggccatca ccctgatacg tatactggcc tgcctgttac
    22021 tgatgagtgc cattgcagca ctgtgtgcct caaaaaccct gattgtgtca taccgcctct
    22081 ggcaactgaa tgaacggcgg gtcagcgagg acgaacaggg caccttcccc gattacctgc
    22141 aggcccggca cggctggaaa caggccacat ggctggccat tacccaccgg cagtactgac
    22201 cgtccggtct gcaattcctt ttcctcattc cggaggctga tgccctgcat cccttcggtt
    22261 ccggagttat cgtgaaaatc atacccagag cactgagtgc gggtctggcg ctggctgccg
    22321 tacctgcaca ggcattcgtc acctatcagg acattgtgag cgccgccacc aatccggatg
    22381 acctgtcgcg ccaggcactg gtcaccattt ttggcgacgt ggtcacaaat cccctgacag
    22441 ccagcagcaa caccattatc ggtaacctgt tcatggtgtt taacgcaatt atcgccatta
    22501 tggggatagt ctggttcctg tttattggaa tccgccatgt tgtccgcacc ggccaccagg
    22561 gcaatgtctt taacaacgga cgggacgtcg tcggaatgct cagtattgtg agcggtttta
    22621 tgatgattgt gcccaccgct tccggctgga gcctggcaca actggtcatg ctctggggcg
    22681 catccatcat gggcattggt tccgccaata tcatggttca gacggctgca gaaaacatcg
    22741 ctgaaggcta ttcaatgacc gtacagccag ttcatgtctc cacccgcacg gcagcacggg
    22801 gaatactgga gatggcgctg tgcaaacacg ccatgaatgc aggcctcgat gactttaacc
    22861 gtactgcccg ttcatcaacg ccccccatga gcgaatcctc acggacagag aatggcagat
    22921 ataccgtcac cgtctcaaat ggcagtgcca cctgcggtag tgtgagtctc tccgtgaaag
    22981 ggaatggcag caacaaccag tccgcactgg actatttttt caatcccttc attgaaacgg
    23041 agtatcaggg tgtcctgacg gcacaacgct ccgccatgga taccatgctc cgtgacctgg
    23101 acagcgcagc acaggaattt gtgtcagcgt accggacccg acgcgacacg ggtaacggca
    23161 acctgccgga tattgaaagc cgtattcaga aggctgccaa ccggtatgaa gaaacggtgc
    23221 agaaggcgtt gccaacagat aacgcagaaa ctgagcgcag ggagcagctg aaatcctacc
    23281 tgaccaccta cggctgggtt gccctgggcg cctggtacca gacctttgcc accgccaacc
    23341 agcgactgga agacctggcc aaccgcgctc ctactgtgag tggcgtctcg tcactgggcg
    23401 aagccgggga tacggagttc ctgaaggaag tcaagggggc ttaccaggca cagctacaga
    23461 actcaacgta tactcctgca ctgggaacat taaaatcagc aaatgaatat aacagcgcag
    23521 gcagtaacga tccgcaaagt gctattttaa gttcaattcc tggagtagaa cttacaaata
    23581 atgtagcaac aatctttaca aaaaccggca caaccactga ccaactaaac ccactgataa
    23641 aaatgaaaaa tatcggtgat tacactatgg gcattactga aagcatctgg gcgggttata
    23701 ccaccgtcag ggtgctttcg actgccggca gcaactccat ttttggtcga ctagttaata
    23761 ccgttacggg cttacctgag gctgcaaagg cttttatgga agcagccgct ccaccgctct
    23821 actttctgct gtttttgctg ttctgtgccg gcttttcgtt atccatttac ctgccgttta
    23881 ttccgtttat cttctggatg accggcattg gtaactggat agtcagcgtg ctgattggat
    23941 gtgctgctgg ccccctctgg gcggccaccc acctgggaac aagccaggac cggggaagcc
    24001 gggccgctta cggttacatc tacctgatag acagcatgat cagaccgctg ctgatggtat
    24061 tcgggttctt ctttgcgtca gtcgctatca ttgctgccgg aaccattcta aattcgttgt
    24121 ttggagctgc actggccaat gtacagacag actccctgac ggggctattc agcatggcag
    24181 gatttttgct gatttatgcc cgagtctgta ccacgctggt tgcggccgtt tttgctcttc
    24241 aggcttatct gcctgaccat gtgattaatt ttctgggcgg acgtgacggt gttaatatgc
    24301 tggggaatat ggtttcatcc gtcaaagata ttattgccgg aggtaatacc aacatgcgca
    24361 gaacacctgg cgttcgtgaa gaaaaactga aaaatattaa tagagatgat aaagacggaa
    24421 taaaacaata aatgaaaata tcgaaaaacc actaaaatac cattgccata ttcacctgaa
    24481 gagattaaaa tacattcagg tgaatatgga ggtttattat gagtacaaca aaaaacagat
    24541 atccttcctg gtgggcatat tattacctga tccgcaatgc ttattttatt attggaatac
    24601 cattcctatt aatatatgca atattaggta cattaagtat tagctacgcc agatactcct
    24661 cccctaatat ctatatgata acatatggat gttcaacgct gattatcctc ccattactct
    24721 ggctatatct gaaaaccaga gcgaagaaaa agaaagtcct gcaggttgtt cagcagataa
    24781 aagaaagcag tccctttgcg ccgaccagcg attacgagca actgtcattc tcgaagagtt
    24841 gttatttcgg gatcgacatt aaaaacggca ccatgctgta tgtgcgcatt tacccaaaca
    24901 atgtcatgga tgtgattggc ctggatattc acaacttcac ccgaacagtt gctgaagacg
    24961 gcaagctgga aatccacacc acctatgtga gtctgccgat gatccctctt gaaatctccg
    25021 gcatctcagc aaggacgctg gccaacacca tacacaccat ggctgcacgt ggatatgagt
    25081 acaaagagcg tttcccgcag atgatccggt atcgggttaa agagtgggaa aaagttgccg
    25141 gtgtgcctgt tgcagaagta ttttaaggta gcgaccaact gttagacgat aatccgtcag
    25201 ccggttttct ctgaaccggc tttttctttt caggcagaat gcctgtctgc cgtccctgta
    25261 tcagactgta attagcacaa tacggcaatt tgccgttcag tatattcagg aagtacaaat
    25321 catgccatct gcaacacaaa ctgcccgact ggaagcaagg atcagcacag aactgcatgc
    25381 tatgctgaaa cgcgctgcag aaatccaggt aaaaaagggc gcattgaggt ttgctttcgc
    25441 aacaatccgg cggcagattt cctgccgccg tgttgcccgt taacgtttaa cttcgtaggc
    25501 taacgttgcc acaagcactg tgtttccctg ctcgacacgg aatccgcaaa gcgaatccct
    25561 cactatccag acaaaacaca gtatcgtcac acagacgaca attaacattg ttaaaaacgt
    25621 tcgttgtggc atcgcagccc ctccattgcc tttcggccgt catcgggcta ctataatgtt
    25681 gtctaggcat tacagtgggc cctgggttga ttgataaaaa aatcgcctgg ggctttctgc
    25741 tgcatgccgt ccacaaaacg ctttcagcat acagcctcaa gcgcccgccg ctattctaat
    25801 cgattttcag tctgccacaa tcgttttcca cctaatgaca tgttatgcac aacagtattt
    25861 ttacgggaaa ctacacggca aggattcccg gaacacgttc attatttgat ggttacagac
    25921 atcatgttaa atccggaagt aataatgacc tgaaaggaaa tactatgagt aacgaatatc
    25981 aaagcaccag tataaacccg gatgtttttg aacaatttct taccctcctg aatacacact
    26041 gtaaatcatc agaagctgca cttgccatgc tgagtacact ctgctacgaa ctgatgagga
    26101 ggagtgggat ccattcactg gacagcctga tgctcaacgg agaaggtata ttgatacaat
    26161 tacagggggc tgatattcgc gaaaaaacag gtcagcatat tcactgatcc aggtatgggc
    26221 tgaaaacagt gtcagcctga aataaacgct caatgcctca tacacatgag gcattgagct
    26281 cggtcataaa taatctttat tatttttctg attcagcatt tccggtcagt atcggctctt
    26341 ctggcaaaga cattaccgga acgtcacgtt caccattgaa tacaaaaaca ctttcagcga
    26401 aatcattctg taacaaagaa aaacttaacg gcagattttt ggtttgtatc cctgtgatcc
    26461 attgaaaaat ctcgggaaaa cgacgtgcac tgatatgcac atgattgagg tggtgctcag
    26521 taatatcact ggccatcatc atgagaggta catcgtatcc cgaacgcaaa cctggcgcat
    26581 gatgcacagg ggtgctgtct gaactgacgg attgcccatg atcagaaaaa tacagcatgg
    26641 caaaatcatc gccatgctct gtcgtcatgt tataaattgc ctgaagaaac cgatccagct
    26701 taagcaatgt tgataaataa caggatactt tatttccgaa taattccctg aacggctgtg
    26761 catcattaaa aagtctgtca cagggattag gatgggaccc cagcatatga atgaaaatca
    26821 cttttggaga agacgaatta acagcttccc tgacatactg aagcatatct tcatcatcaa
    26881 tgcgacggga agaagagctc ccttccttaa gaaaatattt cttatccgca ttggcggcga
    26941 tgacagaaat cagcgtatca tgtcgcccta aagagccctg atttgatatc caccacgtct
    27001 catatccggc ttttctggca agcgtaacaa tattattacc cggattacct gtttcagtgt
    27061 aatcaagagt cagtgttctg ctgagtgaac ctatcgtatt cggagctgcg gagaaatacc
    27121 cattaataaa tatacccgga gcagaattta accatggtgt tgtcggcaaa ggatacccat
    27181 aaacagaaag ataatcccgt ctgacacttt caccaattac gataattatg gttttatatt
    27241 tcttctccac attcacaata tcccatgaat ctttctgatt gtgtttcagc gattcatatc
    27301 gcatcatttc ttctctggtt tcagctatcc ctgtcattac attcgtaacc atacgttttg
    27361 gccatgcatt tactgacaat acgataaaga acaacagaaa gataaatccc cggtggcttt
    27421 tatcacgctt acgccagaac cagaatgtaa aagcagcaat gatcagtgaa acaataaaat
    27481 aaagaacagg tatgcctgtg ataaattcac ctgcctcatt tagatttgtc tgcattacag
    27541 acgcaataaa actgttgttt atttttccgt aaatcatccc aaccggagca tagaatgcac
    27601 ttaacaaaag aaagatacag acacacgttg ctgaaataac attctgccgc cccaacaaca
    27661 acatcaggaa caatataagc acattcccca gggtgttttt cactgtatat ccgcaacacc
    27721 atgtaacaag cacagaaaac aacagataaa tcagtgactc tttacaccac aacataaaat
    27781 aaaactgttt tgctctctca aaagcatatt ttgatttttc tttaatacaa gacacgccag
    27841 tcattttcct gtacaaacac agacgatcat ttcaacgcat ggatatttat atgaaacagt
    27901 tcacaaaaat accctaccct gtccagcagg gtttacatat tatcaggttc tgtgtttaca
    27961 gcaactgctc cccgaaccag attattcgca taacccgatg aaggcctcat aaccataaaa
    28021 cactcagaat acatttagtt aacaaaaata tgtcttgccc tcccggaaag gcaaacatac
    28081 gaaagagaaa gcatggctat aataatatca atcaaaatat gctcaatctt tccggttgtc
    28141 atacactcag caccgacaac ataaagacaa taaacaaaag tgatagcata gtgtgatata
    28201 taaagaatat aaatgcacag aagtaatttc ccgacaactc ttttccgttt aaagaaagaa
    28261 taaatgaaga acaaacataa agccgccaga aaaagaaagg tagctatgga tattataaaa
    28321 tactccgtca cctgtgaatt ctcatccaga gatgaataca gcgaataata tgtggttgtg
    28381 agattaagtc ttgttatcaa tatataaaca actgataata gtataatata aagaatcaga
    28441 ccaaatctgt ttttctgcgc tccggttaat acttctgatt tatctcgcat aaaatatcct
    28501 gtctgtaatc tgtcccggta aacaatggta aatttcagaa tcagtgcgaa tacggtaaca
    28561 aagattcctc ctgtgcttta aagtgtgtag gcagaaacca gtctggtggt tccggtcagg
    28621 gagtctgata cctcaacttc ccgggtgact ataaccatac ctgactgacg cagtctctgc
    28681 acatcagctg caattcgctg agttcccagc cagaacaggc catccagtgc cgttaccagc
    28741 aacccgcttt ccagcgccag tttcagtcgt gccgtcgggg ctttaacctg cccggcgata
    28801 tcagtaaaac aacgcgtcgt catgccatag cagtccatcc gccgtattcg cgtcgcaaga
    28861 ctgtagctga aacaatccgg cacgccggat aagtcattct gcagactgta tacacatccc
    28921 cgctgccgcc cgttgaacag ggcttttttc tgactgagca gccattcccg acgcagagtg
    28981 tcaatcatct gcggtgtccg ggttaacagc agacgaaacg gcagctcaaa actggtcaca
    29041 taatcaacat tcagtaatgc tgtccgcagt cgctccctgg cacctgcccc ggtctcccgg
    29101 cattcacgca tgacatcagc ccactgacgc atcagtcgtg gagactcatt cctggccctg
    29161 aactgatatt tctcaatatg ataatcaata cgttcctgca tatcgccacc ggatatctgt
    29221 tactatatca tcatgaatat tagcctactg gaattcatgc tctgcgtgaa gcattacatt
    29281 accggaaaca taaactcaac ttaacgatat tattccgtaa tataaacgcc gcctgttttt
    29341 catgaataaa aaaggatgta ataaaatgat aaaatatatg ttctctctcg ctattatttt
    29401 ttcaggttcc ctctgcgctg cacagattca gggaaacgtt atccgcgttc ttgatggtga
    29461 tacactggaa atattacagg ataaaaaaac tgtcagggtc cgcctggcaa atattgatgc
    29521 accggaaaag aaacagccct ttggacgctg gtcagccact cagctgaagg acctggtggc
    29581 cggaaagccc gtaaccgtgc aatacgcgca gacagacagg tatggccgta ttctcggccg
    29641 ggtctatacc gctgacggca cagaagcaaa tcgctggcag gtccgtaacg gcgctgcctg
    29701 ggtgtacccg gactacaata atgactatac tctcccgccg ctccagcgtg atgcccgcgc
    29761 cctgaagcgg ggattatggg cagataaaaa tccggtacca ccatggacct ggagaaaaca
    29821 gaataagaaa taattatttg ctgatatgaa gaaaaaaacc atttcaaaga aaataaatgg
    29881 caaaaataca gcatggaaaa aaattgccat ttttaccgcc gcgttatttt taatatttgc
    29941 tgtctgctgg tcgtaataag cagaataaca gaaaggtgta aaaatgaacc tggttgatgc
    30001 atttgtcact aaagtgatat ccggaccgta tgaagaatac ggaaaatggt gggtggatgt
    30061 agaatatatt tcctggggag ttccgggtaa aagccggctc atgtttgact cacgggaaca
    30121 ggcacaggaa gtgcaggaag gttttcgatt tttaacctga gcatattttg ttaaagggca
    30181 gaaggcggtg accctctgcc cttaagtacg tttccgggat tatcagtcaa ccagttcccg
    30241 gatcagttcc aggattgcta tcactgcgtt agccacagca acaaccgccc tgagaacgat
    30301 gattaacagg ttaatcatcc gcttctccat caatggagaa agaacaccgt ggtttgccct
    30361 tacattgcct gtcgccacgt tttctatagt gtcagttata gacagttctt ctccggccag
    30421 agcaccaaaa ccagcaccac ctgatgtttt ggccaggact tgcaacctgt tcatcccggt
    30481 gaacgatatg ccggaaagaa cgtgcctcaa agctgacaca ccgagtatat acttatactg
    30541 tgcttgcaga aatattattt cagccgtatt atttcatagt cagttatagg cagttatctt
    30601 ccggtcaggg ctaccaactt cgatccggaa tcgcaaaaaa accggcctca ccagtcggtt
    30661 tttttgcgtc tgtacctcct ccccggacag cagttctctc actcccgttg aatatttcct
    30721 gttgaatttt acgaaaatac ggcaaatggg agatatcgaa tatatttcct tgggtgtagt
    30781 cggtaaaaac cagctcatgt ttgactcaca gaaacaggct ctggaagtac agaaagtaat
    30841 ctgtccgtag attattaaag ggcagagggc ggtgaccctc tgcccttaag tacgtttccg
    30901 ggattatcag tcaaccagtt cccggatcag ttccaggaga gctatca
//
LOCUS       DAZFRJ010000040        30281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000040 DAZFRJ010000000
VERSION     DAZFRJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.040"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..1657
                     /gene="yegH"
                     /locus_tag="ACHKUN_001611"
     CDS             74..1657
                     /gene="yegH"
                     /locus_tag="ACHKUN_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6659897.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(1931..3784)
                     /gene="asmA"
                     /locus_tag="ACHKUN_001612"
     CDS             complement(1931..3784)
                     /gene="asmA"
                     /locus_tag="ACHKUN_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO6659898.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3806..4387)
                     /gene="dcd"
                     /locus_tag="ACHKUN_001613"
     CDS             complement(3806..4387)
                     /gene="dcd"
                     /locus_tag="ACHKUN_001613"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO6659899.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4479..5120)
                     /gene="udk"
                     /locus_tag="ACHKUN_001614"
     CDS             complement(4479..5120)
                     /gene="udk"
                     /locus_tag="ACHKUN_001614"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO6659900.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5438..8748
                     /gene="dgcE"
                     /locus_tag="ACHKUN_001615"
                     /pseudo
     CDS             5438..8748
                     /gene="dgcE"
                     /locus_tag="ACHKUN_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(8787..9644)
                     /gene="alkA"
                     /locus_tag="ACHKUN_001616"
     CDS             complement(8787..9644)
                     /gene="alkA"
                     /locus_tag="ACHKUN_001616"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO6659901.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9778..11130
                     /gene="yegD"
                     /locus_tag="ACHKUN_001617"
     CDS             9778..11130
                     /gene="yegD"
                     /locus_tag="ACHKUN_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6659902.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11163..13082)
                     /gene="yegI"
                     /locus_tag="ACHKUN_001618"
     CDS             complement(11163..13082)
                     /gene="yegI"
                     /locus_tag="ACHKUN_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO6659903.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(13079..13840)
                     /gene="pphC"
                     /locus_tag="ACHKUN_001619"
     CDS             complement(13079..13840)
                     /gene="pphC"
                     /locus_tag="ACHKUN_001619"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO6659904.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13837..14496)
                     /gene="yegL"
                     /locus_tag="ACHKUN_001620"
     CDS             complement(13837..14496)
                     /gene="yegL"
                     /locus_tag="ACHKUN_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6659905.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14717..14776)
                     /gene="ibsA"
                     /locus_tag="ACHKUN_001621"
     CDS             complement(14717..14776)
                     /gene="ibsA"
                     /locus_tag="ACHKUN_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO6659906.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15049..15105)
                     /locus_tag="ACHKUN_001622"
     CDS             complement(15049..15105)
                     /locus_tag="ACHKUN_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6659907.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15377..15433)
                     /locus_tag="ACHKUN_001623"
     CDS             complement(15377..15433)
                     /locus_tag="ACHKUN_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6659908.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15712..16959
                     /gene="mdtA"
                     /locus_tag="ACHKUN_001624"
     CDS             15712..16959
                     /gene="mdtA"
                     /locus_tag="ACHKUN_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO6659909.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16959..20081
                     /gene="mdtB"
                     /locus_tag="ACHKUN_001625"
     CDS             16959..20081
                     /gene="mdtB"
                     /locus_tag="ACHKUN_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO6659910.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIASYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20082..23159
                     /gene="mdtC"
                     /locus_tag="ACHKUN_001626"
     CDS             20082..23159
                     /gene="mdtC"
                     /locus_tag="ACHKUN_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO6659911.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23160..24575
                     /gene="mdtD"
                     /locus_tag="ACHKUN_001627"
     CDS             23160..24575
                     /gene="mdtD"
                     /locus_tag="ACHKUN_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6659912.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24572..25975
                     /gene="baeS"
                     /locus_tag="ACHKUN_001628"
     CDS             24572..25975
                     /gene="baeS"
                     /locus_tag="ACHKUN_001628"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO6659913.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            25972..26694
                     /gene="baeR"
                     /locus_tag="ACHKUN_001629"
     CDS             25972..26694
                     /gene="baeR"
                     /locus_tag="ACHKUN_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO6659914.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26885..27217
                     /gene="yegP"
                     /locus_tag="ACHKUN_001630"
     CDS             26885..27217
                     /gene="yegP"
                     /locus_tag="ACHKUN_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO6659915.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27426..27722
                     /locus_tag="ACHKUN_001631"
     CDS             27426..27722
                     /locus_tag="ACHKUN_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6659916.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27724..28020
                     /locus_tag="ACHKUN_001632"
     CDS             27724..28020
                     /locus_tag="ACHKUN_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6659917.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28123..29484
                     /gene="yegQ"
                     /locus_tag="ACHKUN_001633"
     CDS             28123..29484
                     /gene="yegQ"
                     /locus_tag="ACHKUN_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO6659918.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29814..30131)
                     /gene="yegR"
                     /locus_tag="ACHKUN_001634"
     CDS             complement(29814..30131)
                     /gene="yegR"
                     /locus_tag="ACHKUN_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO6659919.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7007 a         8067 c         7985 g         7222 t
ORIGIN      
        1 tgcatttttg ccccgattta tccatgatcg aattatgaca tttgtcatac aacgaatagg
       61 ttttgtactt actatggaat ggattgccga tccgtctatc tgggccgggt taatcacgct
      121 gattgtgatc gaactggtcc tcggcattga taacctggtc tttattgcca tcctcgccga
      181 aaaactaccg ccgaagcagc gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt
      241 aatgcgcctg ttactgctgg cgtcaatctc ctggctggtc accctgactc aaccgctgtt
      301 cagcttctgc tcgtttacct ttagcgcccg cgacttaatc atgctgtttg gtggtttctt
      361 cctgttgttc aaagccacga tggagctgaa cgaacggctg gaagggaaag acagcaataa
      421 tcccacacaa cgcaaaggtg cgaagttctg gggcgtggtg acgcaaattg tggtactgga
      481 cgccatcttc tcactcgact cagtgattac cgccgtcggg atggtcgacc atttactggt
      541 catgatggcc gccgtggtta tcgctatcag cctgatgttg atggccagca agccgttaac
      601 gcaatttgtt aacagtcacc cgaccatcgt tattctctgc ttaagcttcc tgttgatgat
      661 tggctttagc ctggtggcag aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc
      721 tgccattggt ttctcggtga tgatcgaggc gctcaatcag ttggctatct ttaaccggcg
      781 acgttttctt tccgctaacc agacgctgcg ccagcggact accgaagcgg taatgcgcct
      841 gcttagcggg caaaaagaag atgcggaact ggacgccgaa accgcgtcca tgctggtgga
      901 tcatggtaac cagcagatct ttaatccgca ggaacggcgg atgattgagc gggtacttaa
      961 tcttaaccag cgtactgtca gcagcattat gacgtcgcgc cacgatattg agcatatcga
     1021 tctcaacgcg ccggaagaag aaatccgcca gttgctggag cgaaatcagc atacgcggct
     1081 ggtggttacc gacggtgatg acgcagaaga tttgctcggt gttgttcacg ttatcgacct
     1141 gctacaacag tcactgcgcg gcgaaccgct caacctgcgg gtgttgattc gccagccgct
     1201 ggtgttcccg gaaaccttgc cgttgttacc tgccctggag cagttccgta atgcccgcac
     1261 gcactttgct tttgtagtgg atgagtttgg ctcggtggaa gggattgtga cattaagtga
     1321 cgtcactgaa accattgccg gtaacttacc gaacgaagtg gaagagatcg acgcccgcca
     1381 tggtattcag aagaatgcgg acggttcctg gacggcgaat ggtcatatgc cgctggaaga
     1441 tctggtgcaa tatgtgccgc tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg
     1501 gctgttgatg gaatatttac agcgtattcc aaaacctggc gaagaagttc aggtggggga
     1561 ttatttgctt aaaacgttgc aggtggaaag ccatcgcgtg cagaaggtgc agattattcc
     1621 gctgcataag gatggcgagt tggagtacga ggtgtgatgg tatcggtcat tttacccgat
     1681 agccgaaagg aataagatga gggaaccgcg ccagcggcga tttaccgccg ggagcccggg
     1741 ttgccagggt ggtggcgatt gagccaccct ggcacgttca ccggcttgag cgttccatag
     1801 tagtgaggaa catcaagtga acggaacagc cttcgatctt acatattcag aatataatgg
     1861 tgccgccgcg aacgcctgat gcgacgcttt acgcgtctta tcaggcctac aggacaggtc
     1921 acctggtaac ctacatcttc tccagcaact tcttcacatc tttgccattg cgggaatctt
     1981 tattccgctc ggcccagtca ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca
     2041 gtaactgatc cacttgcaga ctgtaattga gttgctgcca gttgccataa acccgcagcg
     2101 gtactggcgt ttctttgagg aaatcaatca gtttgctttc cccgttccag ccacccacga
     2161 cccgaatatc aaactgggtg tcgcaggttt gatctgccag attcaacatg ccttcccctg
     2221 tcagcgccag cacaggcgat tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca
     2281 acgtcaaatc ggtggtaaag cggtcaagac gcgttacgtt atcgaagttt tcagcggcct
     2341 tcacatcacc accattacgt tctaccgctt gctgaatcat ctgctggaag ttcatccctt
     2401 ccatgcgcgt gtcggtcatt tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt
     2461 cggcatctat gtcagcaccg gagaaatcac cagccagtga catttttccg gtcaacgaaa
     2521 tcggatagtt aaacgccttc agaatggtac caatctcaac gttttccagc cgtggctgga
     2581 agtttatccg cggatttatt gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac
     2641 cgttaagctt gccctgcagt tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg
     2701 tggcaacatc tgtaaaattc attccgcgcc agcgcacgtt actggcctgc aacaaaatat
     2761 cagccgtaaa gccttgcagt ccctgatatg ccggttcatc aatacgcgaa gaaatgaccg
     2821 ggcgcggcaa tgttgattgg ctctgcccct gctgcgcggc accgttttca ccattcgctg
     2881 tttcattaag cgggatgagg ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc
     2941 attcaggttt ctctgtcagc gtgacctgtg cttgcccgct cagcgtgctg tcattggcgg
     3001 tcaagctgat ttggttaaat gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac
     3061 tcccctgccc ctgaatacct tgttttggta aatctgcacc ctgcaactgc cagttaattt
     3121 gttcaatagc cgccgttaaa tcatgcggat aatcagaagc atctaccgta ccgttaaggg
     3181 atatcgtgag atcgcgctga tcgcgattaa cccgcccgga gaactcaaat gagccacgat
     3241 gttggggatc ttgttccatt tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt
     3301 catgctggaa caccagcacg ctgtccgcca ccttaagact ggatatatca aacgaccatc
     3361 cgcgatcgtc tgacagatcc ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac
     3421 tgcgcaccgc ttccgtctgc ggcgtcagtt ggatcactgc cccttttagc atcacctgct
     3481 taacgctcag ttgatgactc agtagtggta aaagcgccac gtccagacgc atgttgtcgg
     3541 cgcgaaccag tggctggctt gcgccctggg cggtgagaga cattcgcccg gagaggatac
     3601 taagctgcgg ccagacgtgc caacgcagtg gcccgtcgag ctgcaattga taaccgctac
     3661 gtgcagcaac ttgcttgacc atatagtcgc ggaaatcatt cggattcacc agcaacacta
     3721 acgcagataa cccggccacc agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc
     3781 tcatggtatc ctcaatgggc ctgaattagt ctttatcgat tcggctggct accgcgccct
     3841 gctggttgcg atatttcgca tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa
     3901 gcggctcaaa gctcagcgca ccaattaaca tgcccggacg cagcgccagc ggcagcttac
     3961 cggagttgta gaactccagc acaatgcaac cagaccagcc cggatcgata cggtgcgcgg
     4021 tgacgtgcac catcagcccc agacgcgcca aagaggaacg cccgtccagc cagcccacca
     4081 gatcggctgg cagcgtcacc gactccagcg tcaccgccag cgccagctct cctgggtgaa
     4141 gatagaacgc ctcgccctcg tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg
     4201 cgctcacttc atctttggga ccgctcagat cgataaacgc tgccgtgtga ccacggaagg
     4261 tacgaaattt attgcccagg cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg
     4321 gacgtgggtt gatcgacaaa cggccttcat caagccaggc ttcaatatct cggtcacaca
     4381 gacgcatggc attttctcct ttcgcgcatc actcccttaa cgccaattac gtcaagggca
     4441 taccaggtta tcactgaacg gtacacaatt tatcaagctt attcaaagaa ctgacttatt
     4501 ttcgctttca atatatcgat cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc
     4561 gcatattgtt tagaaggctc aatgaattgc aggaacatcg ggcgcacggt tttttgatat
     4621 tgcgccatca cggaatccat tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc
     4681 atcaggcaga tatccagcgg ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa
     4741 cgcgcatccg tcagcaacaa aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc
     4801 accgtttctt tcatacgcgt atgttcaaca tagctgtaaa ccggcaggtc aattgccgag
     4861 ccgcgtttca acgcttgtaa atgctcaagc aacaggctgt gatccatcgc gctgggatgg
     4921 tcgtagttgg tcttaacgcg ttcttccatc gacagatggc tttgatcttt gtaatagcag
     4981 tcttcgggaa ttacgccgat gtgttcatca ccgacttgct cacgtaattc acgataaagg
     5041 gtactggcaa taagactctt gccggaagcc gatgcgccag cgataccgat aatgacgcac
     5101 tgatgagact gatcagtcat atatttagcg acctgattaa cctggatgtt aggaaggggg
     5161 cgacgaagcg ccaaacgcgg caattatagg gatttcatcc gcctgatacc agtcgaatag
     5221 cgctgccgcg cgctcagagt taattgttga cgaagaattc ccggtggcaa attacgttga
     5281 tcagttttat acaaggtaaa aaatgttata cgcagttgcg caaattatcc gcctttacgt
     5341 cactttatga gcaattcgca tataaaatgt aaaacttttg tactagcata aacacagaaa
     5401 cgaatactgg cgacctggtc ttgcggataa agcggtaatg agcaaacaat cacagcatgt
     5461 attaattgcc ctgccccacc cgctgcttca cctggtcagt ttaggtttag tctcgtttat
     5521 ctttaccctt ttctcgcttg agctttcgca gtttggcacc caactcgccc cactgtggtt
     5581 cccgacgtcc atcatgatgg tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat
     5641 tgcgctgagc tgctcgctgg gaaatatcgc cgcatccatc ctgctttttt ccaccagctc
     5701 gctgaacatg acctggacga ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct
     5761 acgtaaattg ctgccgtggt ataacccctt gcaaaatctg gctgactggc tgcgtctggc
     5821 attcggcagc gccattgttc cgcctctgct agggggtgtt ctggttgtcc tgctgacgcc
     5881 cggagacgat cctctcaggg catttttgat atgggtactg tcagaatcca tcggcgctct
     5941 ggcactggtg ccgctgggat tgttatttaa accacactat ctgctgcgcc atcgcaaccc
     6001 acggttgctt tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa gctggctttc
     6061 gatgctgtat ctgccgtggc cttttacttt cattattgtg ctgttgatgt ggagcgccgt
     6121 gcgcctgcca cgaatggaag cctttttgat cttccttacc acggtgatga tggtgtcgct
     6181 gatgatggcc gcggatccct ccctgcttgc tacgccgcgt acatacctga tgagccatat
     6241 gccgtggcta ccgtttttgc tgatcctgct gcccgccaac atcatgacga tggtgatgta
     6301 tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa acccgttttc ggaacgccat
     6361 ggaatattcc gctatcggta tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa
     6421 caaagcgctc tgccagtttc tcgggtacag tcaggaagag ctgcgcggac tcacctttca
     6481 gcaactgacc tggccggagg atctcaataa agatctccaa caggttgaaa agctgataag
     6541 cggtgaaata aacacctatt caatggaaaa acgctactac aaccgcaatg gcgatgttgt
     6601 ctgggcgttg cttgccgtct cactggtgcg ccacacggat ggcacgccgc tctattttat
     6661 cgctcagatt gaagacatta acgagctaaa acgcaccgaa caggtgaatc agcaactgat
     6721 ggagcgcatc acgctggcta acgaagcggg cgggattggc atctgggagt gggagttgaa
     6781 gccgaatatt tttagctggg ataagcggat gttcgagctg tatgaaattc ctccacatat
     6841 caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga
     6901 aaaagtgatt cgttgcaatc acgctcgccc tttaaactgg aatttcgcat taccgtaaaa
     6961 gacggtattc gccatatccg cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc
     7021 gaacgcctgc tcggcattaa tatggatatg actgaggtta aacagcttaa cgaggcattg
     7081 tttcaggaaa aagagcgcct gcacataacg ctggactcca tcggcgaagc cgtggtctgt
     7141 attgatatgg caatgaaaat tacctttatg aatccagtgg cggagaagat gagcggctgg
     7201 acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac ctttggcgac
     7261 aatggtcccc tgatggagaa catttacagt gccgacacct cacgttccgc gattgaacaa
     7321 gatgtggtgt tgcactgccg gagcggcggc agctacgacg tgcattacag tattacgccg
     7381 ttaagtactc tggacggcag caatattggt tcggttctgg tgattcagga cgttaccgaa
     7441 tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact gacgcatctc
     7501 gccaatcgcg ccagttttga gaagcaactg cgtatgctgc tgcaaacggt aaacagtaca
     7561 catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt gaatgacagc
     7621 gccgggcatg cggcgggcga cgctttgctg cgcgaactgg cgtcgttgat gctgagtatg
     7681 ctgcgttcca gcgacgtgct ggcgcgactc ggtggggatg aatttggtct gctgctacca
     7741 gactgtaatg ttgaaagcgc gcgttttatc gctacacgca ttatcagtgc cgtgaatgac
     7801 tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg gattaccttg
     7861 attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat cgcctgttat
     7921 gcctccaaaa atggtggacg gggccgggtg acggtttacg aaccacagca agctgccgca
     7981 catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa agagaatcag
     8041 ttgatgatga tggcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg taatttgtgg
     8101 ctgatttcgc ttaaactctg gagttgcgaa ggcgagatta ttgatgaaca aaaatttcgt
     8161 cgtagcttca gcgatccggc acttagccat gctcttgacc gacgggtatt ccacgaattt
     8221 ttccagctgg ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct ccccctttcc
     8281 gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct ggaaaatagc
     8341 cctctaccac cacggttatt acatctgatt attccggcag acgcgatttt agatcacgca
     8401 gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag tgctcagcca ggtgggccgc
     8461 gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct acttgatggt
     8521 gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac gattattcag
     8581 gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt acccttagtg
     8641 atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgatgtgat tgccgatgcc
     8701 caaccgctgg atttgctggt gaatagcagt tatttcgcga ttaactgagg gagcgtttgc
     8761 ccattgcctg atgcgacgca tcaggcctac aattcgtccg ttccgtctgg ttgccagcct
     8821 tccgtatacc agatatgcaa cagcgcataa gaacgccagg gcttccagcg ctcggcataa
     8881 cggcggattt gtgccggtgt catccccgga aatcgctgtt taatcagata atcatccggc
     8941 agaaaaacat ctttcgcctg ccagccacgc aaagcgaaat aattcgccgt ccagcgcccg
     9001 atacccggaa aagtttgcag cgttttcatc gcctgctcca catcgcccgg aattgtcatt
     9061 ggtaagctgc cctccagcgc cgcatttgcc agatgaatca gtgcctctgc ccgtttcaac
     9121 ggcatgccta acgcttttaa tgcctgcggg tcggctgctg ccagccgctg aggcgtgggg
     9181 aagcagatat actccggaaa atcatccagc cgttcgccat aaagctgtac aactttggca
     9241 gtcaattttg ccgccatcgc cacgctcacc agttggccta aaatcgcccg cacaccctgc
     9301 tcaaaagcat caatacagcc gggtaaacgc aatccgggcc gcgccgcgcc taacttgccc
     9361 aacgcaccgt taacaatctg cgggttacat tgcagatcaa acaggcggct cattttcgcc
     9421 agacactcag cggcaacggg ttctaaacct gcacttaaat ttatgtgcag agtatggcgg
     9481 gctatatccg gaatagcagt caccacgccg cgatattcgc ccaccgccag actacgggca
     9541 taataatcct cagcgaccgt ttccacacca ctcactgcac gggcggcgag aaatcccaac
     9601 atccacgacc agtcatacgg cggctgccag ttcagggtat acatcgcatc tccttattca
     9661 tccgctttca gcataaacgt tattcagacg ctacgctttg ctttcatatt ccggttgtcg
     9721 cgacagcaac atttcgctaa agtcacgccc cttcttcacc ggcatgggga ttaattcgtg
     9781 tttattggtt ttgattacgg tacagcaaac tgttcagtgg cggtcatgcg tgacggtaaa
     9841 ccgcaattac tgaaaatgga aaacgacagc acgctgctgc cttcaatgct ttgcgcgcca
     9901 acgcgtgaat cggtaagcga atggctgtac cgccatcatg atgttccggc agacgacgat
     9961 gaaacgcagg cgctgttacg tcgggcgatt cgttataacc gcgaagaaga tatcgatgtt
    10021 acggcgaaaa gcgtgcagtt cggtctttcc tcactggcac agtacattga tgatccagaa
    10081 gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg ccagcggctt aaaaccgcag
    10141 caggtagcgc tgtttgagga tctggtctgc gcaatgatgt tgcacattcg ccagcaggcg
    10201 caggcgcagc tgccagaagc gattactcag gcggtgattg gtcgcccgat caacttccag
    10261 gggctgggcg gcgatgaagc aaacgcccag gcgcaaggga ttctggaacg cgcggcgaag
    10321 cgtgccggat tcagggacgt ggtattccag tacgagccgg tcgcggctgg gctggattac
    10381 gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg atatcggcgg tggtacgact
    10441 gactgttcat tgctgctgat ggggccgcag tggcgttcgc gtctcgatcg tgaagccagc
    10501 ctgctgggtc acagtggttg ccgtattggc ggtaacgatc tggacatcgc actggcgttt
    10561 aaaaacctga tgccgttact gggcatgggt ggcgaaaccg aaaaaggcat cgccctgccg
    10621 attctgccgt ggtggaatgc ggttgccatc aacgacgtac ctgcgcagag tgatttctac
    10681 agtagtgcca acggtcgtct gcttaacgat ctggtacgcg atgcccgcga accggaaaaa
    10741 gtggccctgc tacagaaagt ctggcgtgag cgtttaagct atcgcctggt gcgtagcgca
    10801 gaagagtgca aaattgctct ttcaagcgta gcggaaaccc gcgcctcact gccgtttatc
    10861 agcgatgaac tggctacgct gattagccag caagggctgg aaagcgccct cagccagcca
    10921 ctggcgcgga ttctggaaca ggtgcaactg gccctggata acgcccagga aaaaccggac
    10981 gttatctacc tgaccggcgg tagcgcccgt tcgccgctga ttaaaaaagc gctggcagaa
    11041 cagttgccgg gaattccgat tgcaggcggc gatgactttg gctccgtcac cgccgggctg
    11101 gcacgctggg cggaagtggt gtttcgttaa aagaaaatgg ctcagcagcg gcttaaatct
    11161 gcctacgcct gagccagttt ttctgccgcc tgacgtaaag gttccatcaa cggcatggtc
    11221 cgtgctggag catgaaggcg gaatcgctgc aactctgtcg cgccgacagt caacgtcgat
    11281 tccagccgat gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt
    11341 atcgcttcat tcggcctgaa cacaaaacgg cgttcacagc tcgctttcca gtcgataacc
    11401 gcctgggtca gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct
    11461 gcggctgttt caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga
    11521 atagaggcaa catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca
    11581 gtgtcatgaa gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc
    11641 ttcatttttt cgagcatcgc ccgtttggcg ataaaacctt ccagcccgcc caactgttgg
    11701 atctggctga ctaaatgctc atagtccatt ttcgcgcgat taaatgcctc acggcgttgc
    11761 tggatttctg ctttgtacgc tttgcttgtc agactgccaa taatccagat agccgacagc
    11821 acaggaacca aaataatata acgcggttct gcctgaaggc cgcaaagcaa cagcgataac
    11881 gctgacagtg cgatctcaat cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc
    11941 ggcctgccag tcggttggaa atgatcgggt aatggcaatt gcaatgctgg cggtgctact
    12001 gacgccatca ccatcgccca gactttcgcc agcacaaaat caccgccggt ggtaatgacc
    12061 tcttcgccga gatcaataaa atagataacg ccttgattat ccagcgcaca ccacgggcag
    12121 tcggtcaaat gagcgggata aacatgcatt gccgaaacgg gacatttctt taactgttgg
    12181 cgcagagaat caagtgccgc tacccacgct ttagccgtcg gacgcccggt tgccacgcca
    12241 ctttccgtga atgcctgctg aaacatggct tcaacatcgc ccggtaacat cgatagcgga
    12301 atcgatcgtg gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg
    12361 aaatgggtaa tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa
    12421 taaggatgcc ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg
    12481 tgattttctg tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta
    12541 aaatgcgaca cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag
    12601 tcgctatcaa tcagtaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg
    12661 tcacccacga cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca
    12721 acatagagta gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta
    12781 tagatcatat gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt
    12841 ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg
    12901 tcagctgtcg cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga
    12961 taaatcttgg cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct
    13021 ttgcccagtt cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc
    13081 atggtaacca cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg
    13141 ggctggacaa aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg
    13201 acgccagtcc attgaaaaac ggggtaaaga aaggtacatg aggagaatta tccagcatat
    13261 ttaacgccag tcgctggata ccatccgtaa atgctgcaac tttatgcaca cgctcagtgc
    13321 tggtgaaagt ttccagtctg gaaacggcat cttcatcggt aatgaagtgc gtcatattgg
    13381 catattcacc caccatcggc gtaagcggca attgcagacc gtgaccaaaa tccaccacca
    13441 cgccgccatc gccaatctgc ataatcaagg tgccattagc cgatgaaatc aatcccagaa
    13501 acgtgcaggc aaaatcgcga actgctaatt ctttggcttc cgcctcggca aaaagtcgct
    13561 gacgaacggt cagcacaata tccgtggcga ggatatcatt aagccccaat tccccaccct
    13621 gcactttttg cgacatataa gccatcgctt cattgacggc gagcgtcgcg ccttctcctc
    13681 cctgcgaaac gctacctgcg ccatcagcaa ggaacatcac cagtaatggc tgctggtcat
    13741 ttagccaggc aacctgcatc tggcaggcat cctggcaagg caagtccgcg ctgatgtgcg
    13801 atgttccaac agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg
    13861 cgcttccagc acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga
    13921 taaccagcta aacagttcac ggaattgtaa tccttgcaga ggcaaaggct gacgaacgct
    13981 gatttgtgcc agagtcttca tatccgcccc ctgaacaccg atggaaaaga aagcaaacct
    14041 tttatcttct tccccccgaa acactttgtt ggctgcagcc tgccactcat cggttggtgc
    14101 accatcggta atcaggaaaa tccacgggcg ataatacgaa ataccatttg cacgatactc
    14161 acgtttccgc tcctctacca tatccagggc tttggtaatg gccgcaccca ttggtgtatc
    14221 gccctgggca aacaggatgg gcggaaaaaa attagcggcg ctggtaaagg gttgttccac
    14281 atgcaccggg ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcggc
    14341 aagcagttca tcacgaaagg tcaccaatcc ggcgttaagt tcattaatag gtctgccgct
    14401 catcgagcca gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc
    14461 aaaatcgctg gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta
    14521 tcgaggtccg gtatcatacc gactgcaata atgaaataag agcaaacgaa gaaactttca
    14581 gaattaggcc cggcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc
    14641 gattgacact aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc
    14701 cctccccctg gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga
    14761 tgacgtgctt catcataacc ctttccttat taaaagccct cttctccggg agaggctttc
    14821 ccgtttcagc gtcccgctga aatcatcggc ttacctcctt tcgccctgaa tgcagtctat
    14881 cgctaacgcg tgaataaaat agtttcctct gtgattattg cgtaacgact cgcaaagaat
    14941 atttgctggc aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca
    15001 tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg
    15061 gaagcttatg actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt
    15121 taaggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt
    15181 acctcctttc gccatgcact tagtctatcg ctgacttgca gataaaatag tttccagtgt
    15241 gattattgcg taacgtctcg caaagaattt ttgctggcag gatcgcagac ggcaaagcct
    15301 gcggattgac aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc
    15361 ccccgccctg gtagtcttag tatgctgggt agctaatcac taagagcacc acgatgatga
    15421 gtagcttcat catgaccctt tccttattta aggccccttc ctcgggaggg gctttcccgt
    15481 ttcagcatcc ggctgaaatc tgcggcttac ctcctttcgc cctgtatgca gtctatcgct
    15541 aacgcgtaga taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg
    15601 ccattcagac gattcccgat agtgtttcat aattcctcca tttttctccc ttattggctg
    15661 gctacactag tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc
    15721 agttataaat cccgttgggt aatcgtaatc gtggtggtta tcgccgccat cgccgcattc
    15781 tggttctggc aaggccgcaa tgactcccgg agtgcagccc caggggcgac gaaacaagcg
    15841 cagcaatcgc cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag
    15901 gcggcgaccg ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattatc
    15961 gccgctaata ctgttacggt gcgcagccgc gtggacggtc aactgatggc gttacatttc
    16021 caggaaggcc agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc
    16081 aaagttgcat tagcacaaac ccagggccaa ctggcaaaag ataaagccac gcttgctaac
    16141 gcccgccgtg atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag
    16201 gagctggatg cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa
    16261 gcaagcgtcg ccagcgcgca gttgcaactc gactggagcc gcatcaccgc accagtcgat
    16321 ggtcgcgttg gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc
    16381 ggaattgtgg tgatcaccca gacgcatcct atcgatttag tctttaccct gccggaaagc
    16441 gatatcgcta ctgtagtgca ggcgcaaaaa gccggaaaac cgctggtggt agaagcctgg
    16501 gatcgcacca attcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc
    16561 gatgccacaa ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg
    16621 tttcccaatc agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg
    16681 atcccaacag ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc
    16741 gaaaacaagg tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg
    16801 atccgtgcag gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc
    16861 gaaggggcga aagtggaagt ggtggaagcc cagagcgcca ccactccgga agagaaagcc
    16921 accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca
    16981 gcacaggcgg cccgtcgcgc ctgtttatta tgcgtcctgt ctccaccacg ctgctgatgg
    17041 tggcgatctt actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg
    17101 aagtggacta tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca
    17161 tgacctctgc cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac
    17221 agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat
    17281 taccgctcga tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt
    17341 tgccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga
    17401 tcatgacgct cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg
    17461 aaacccgcgt cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acgctttccg
    17521 gcggtcagcg tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc
    17581 tgaccagcga aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta
    17641 gcctcgacgg cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg caatccgccg
    17701 aagagtatcg ccagctaatc atcgcctacc agaacggcgc gccaattcgt ctgggcgatg
    17761 tcgcaactgt agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac
    17821 aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca
    17881 gcattcggca gatgctgcca cagctcactg agagtctgcc gaaatcggtg aaggtgacag
    17941 tgctttccga tcgcaccacc aatatccgcg catccgtcga tgatactcag tttgaattga
    18001 tgatggctat cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga
    18061 ccatcattcc cggtgttgct gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt
    18121 ttctcgattt ttcaatcaat aacctgacac tgatggcgtt aactatcgcc accggattcg
    18181 tggtcgatga cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa
    18241 aaccgttggc ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt atctcgctga
    18301 ccttctcact gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac
    18361 tgttccgcga atttgctatt acgctggcgg tagcgatttt gatctcagcg gtggtgtcgc
    18421 tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga
    18481 accgcttctc ccgtgcctcg gaaaaaatgt tcgacaggat aatcgcctcc tatggtcgtg
    18541 gactggcgaa ggtgctgaat catccgtggc tgaccttaag cgtggcgctc agcacgctgc
    18601 tgcttagcgt tctcttatgg gtgttcattc cgaaaggttt cttcccggta caggacaacg
    18661 gcattattca gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc
    18721 gacaacgcca ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat
    18781 ttgttggcgt tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca
    18841 aaccgctgga tgaacgtgat gaccgagtgc aaaaagtcat tgcccgtctg caaacggcgg
    18901 tggataaagt gccgggcgtc gatctcttcc tgcaaccaac gcaggatctg actattgata
    18961 ctcaggtcag ccgcacccag taccagttta ccttgcaggc cacctcactg gatgcgctca
    19021 gtacctgggt gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct
    19081 ccagcgactg gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca
    19141 gccgtctggg gatcagcatg gcggatgtcg ataacgccct gtacaacgcg tttggtcagc
    19201 gactgatttc cactatttat actcaggcta accagtatcg cgtagtgctg gagcacaaca
    19261 ccgaaattac cccgggcctc gcggcgctgg ataccattcg cctgaccagt agcgacggcg
    19321 gcgtggtgcc gttaagctca attgccaaag ttgaacagcg ttttgcgccg ctttccatca
    19381 accatctgga tcagttcccg gtaacgacca tctcctttaa cgtgccggat aactattcgc
    19441 tgggcgatgc ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata
    19501 tcaccacgca atttcagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct
    19561 ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct
    19621 ttattcaccc gatcaccatt ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg
    19681 cattaatgat tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga
    19741 tcggtattgt gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg
    19801 agcaaggcat gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga
    19861 tcctgatgac caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg
    19921 tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc
    19981 aggtgctgac gctgttcacc acgccggtga tttatctgct gttcgaccgc ctggcattgt
    20041 ggaccaaaag tcgctttgct cgccatgaag aggaggcgta agtgaagttt tttgccctct
    20101 tcatttaccg cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac
    20161 tgggcttccg tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg
    20221 tcagcgcctc gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc
    20281 tggagcgctc acttgggcgc attgccggag tcagtgaaat gacctccagc agttcgctcg
    20341 gcagcacgcg tattattttg cagtttgatt ttgaccggga tatcaacggc gcggcgcgcg
    20401 atgtgcaggc ggcgatcaac gctgcacaaa gtttgctgcc cagcgggatg cccagccgtc
    20461 cgacctatcg caaagctaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg
    20521 atacttattc gcagggtgaa ctgtacgatt ttgcctcgac gcagctggct ccgacgattt
    20581 cgcaaatcga cggtgtcggt gatgtcgatg ttggcggcag ctcgctgccc gccgtacgcg
    20641 tcgggctgaa tccgcaggcg ctgtttaatc agggcgtctc gctggacgac gtacgcaccg
    20701 ccatcagcaa tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct
    20761 ggcagatcca gaccaatgat gagctaaaaa ccgccgccga atatcagccg ttgattattc
    20821 attacaacaa cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc gactcggtgc
    20881 aggatgtgcg caacgccggg atgaccaacg ccaaaccggc tattttgctg atgatccgca
    20941 agctgccgga agccaatatt atccagacgg tagacagcat ccgggcaaaa ttaccggagt
    21001 tgcaggaaac cattccggcg gcgattgatc tgcaaattgc tcaggatcgc tcccccacca
    21061 ttcgcgcctc gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc
    21121 tggtggtgtt tttattcctg cgctcgggtc gcgccactat tattcccgcc gttgcggtgc
    21181 cggtttcgct gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc
    21241 tttcgttaat ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc
    21301 tggaaaacat tgcccgtcat ctggaagcgg ggatgaaacc gctacaggcc gcgctacaag
    21361 gtactcgcga agtcggcttt acggtgctat cgatgagtct gtcactggtg gcggtgttcc
    21421 tgccgctgct gttgatgggc ggattgccgg gccgactgtt acgcgaattt gccgtgacgc
    21481 tttctgtcgc cattgttata tcgttgctgg tttctctgac attaacgcca atgatgtgtg
    21541 gctggatgct gaaagccagc aagccgcgtg agcaaaagcg actgcgtggt tttggccgca
    21601 tgttggtagc cctgcaacaa ggctacggca agtcgctgaa atgggtgctc aatcataccc
    21661 gtctggtggg tgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga
    21721 tcccgaaaac cttcttcccg gagcaggaca ccggcgtgct gatgggcggg atacaggcgg
    21781 atcagagcat ttcgtttcag gcgatgcgcg gcaaattgca ggatttcatg aaaattatcc
    21841 gtgacgatcc ggcagtggat aatgtcaccg gctttaccgg cggttcacga gttaacagtg
    21901 ggatgatgtt tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta
    21961 tcgaccgtct gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg
    22021 tacaggatat tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat
    22081 ccgacgacct ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt
    22141 tgccggaact ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg
    22201 tttacgaccg tgacaccatg gcacggctgg gaatcgacgt acaagccgcc aacagtctgt
    22261 taaataacgc cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata
    22321 aagtggtgat ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt
    22381 tcgttatcaa taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg
    22441 cgaatgcccc actatcggtg aatcatcagg gattatcggc ggcctcgacc atttcgttca
    22501 acctgccgac cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc
    22561 agcttggtgt gccttcgacg gtgcgcggca gttttgccgg aacagcgcag gtgttccagg
    22621 agacgatgaa ctcacaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc
    22681 tgggtatcct ttacgagagt tatgtgcacc cgctgacgat tctctccacc ctgccctcgg
    22741 cgggtgttgg agcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc
    22801 tgatagggat catgctatta atcggcatcg tgaagaaaaa cgccattatg atggtcgatt
    22861 ttgcgcttga agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct
    22921 gtctgctgcg ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc
    22981 cgctggtgtt gtcgggcggc gacggctcgg agctgcggca acccctgggg atcaccattg
    23041 tcggcggact ggtaatgagc cagctcctta cgctgtatac cacgccggtg gtgtatctct
    23101 ttttcgaccg tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa
    23161 tgacagatct tcccgacagc acccgttggc aattgtggat tgtggctttc ggcttcttta
    23221 tgcagtcgct ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg
    23281 gggaaagtcc gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga
    23341 tgctgcccgc cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg
    23401 ccatcgtgct gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac
    23461 tgttgctggc acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg gtcggcagat
    23521 tgacggtgat gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt
    23581 tacccggtca ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg
    23641 cgtcgtggca ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca
    23701 cattgatgtt aatgccgaac tacaccatgc agacgcggcg ctttgatctt tccggctttt
    23761 tattgctggc ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag
    23821 gtttatcgcc gctggcgatt gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt
    23881 atctgctgca cgccagaaat aacaaccgcg ccctgttcag tctgaaactg ttccgcactc
    23941 gtaccttttc gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc
    24001 cctttatgac accggttttc ctgcaaattg gcctcggttt ctcgccgttt catgccggac
    24061 tgatgatgat cccgatggtg cttggcagca tgggaatgaa gcgaattgtg gttcaggtgg
    24121 tgaatcgctt tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca
    24181 ccctgttgtt tatgactacc gctctgttgg gctggtacta cgttttgccg ttcgtcctgt
    24241 ttttacaagg aatggtcaac tcaacgcgtt tctcctccat gaacaccctg acgctgaaag
    24301 atctcccgga caatctggcg agcagcggaa acagcctgct gtcgatgatt atgcaattgt
    24361 cgatgagtat cggcgtcacc atcgccgggc tgttgctggg actttttggt tcgcagcatg
    24421 tcagcgtcga cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt
    24481 tgatcatcgc ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg
    24541 tagctatttc gcggcgaaaa aggagcgcgc aatgaagttc tggcgacccg gtattaccgg
    24601 caaactgttt ctggcgattt tcgccacctg cattgtcttg ctgatcagta tgcactgggc
    24661 ggtgcgtatc agttttgagc gtggctttat tgattacatc aagcatggta atgaacaacg
    24721 attacaactg ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt
    24781 cctgcgcaac aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc
    24841 ggaagataaa cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt
    24901 tgatcaaaac aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg
    24961 gcgacccatt ctggtcaacg gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg
    25021 gttaacgcgc aatactgata tcaatttcga taaacaacag cggcaaacca gctggctgat
    25081 cgtcgccctg gcaacgttac tcgcggcgct cgccaccttt ctgctggcgc gcggtttgct
    25141 ggcaccggta aaacgacttg tcgatggcac gcacaaactg gcggcgggcg atttcactac
    25201 ccgcgtaacg cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct
    25261 tgccagcacg ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttcaca
    25321 cgaactgcgt acgccattag cggtgctgcg cggtgaactg gaagccattc aagatggcgt
    25381 gcgcaaattc acgccggaga cggtggcgtc tttacaggcg gaagtcggta cactgactaa
    25441 actggttgac gatcttcatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa
    25501 agcaccggta gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt
    25561 tgccagccgt ggcctgaaac tgcagttttc cctgccagac agtattaccg tatttggcga
    25621 tcgcgaccgt ttaatgcagt tattcaataa cttactcgaa aacagcctgc gctacactga
    25681 tagcggcggc agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt
    25741 tgccgacagt gcgccaggtg tcagtgacga gcagctacaa aaattatttg aacgttttta
    25801 tcgcaccgaa ggttcccgca accgcgccag cggtggttcc gggctggggc tggcgatttg
    25861 cctgaacatt gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg
    25921 ggtaagcatt acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag
    25981 ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg
    26041 cagttgctca ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc
    26101 gatcaggtac tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt agatctgatg
    26161 ctccctggca ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc tgacattccg
    26221 atcgtgatgg tgacggcaaa aatcgaagag atcgatcgcc tgctggggct ggagattggc
    26281 gcagatgatt atatctgtaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc
    26341 attttgcgcc gttgcaaacc gcagcgcgag ttgcagcaac aggatgctga aagcccgttg
    26401 attatcgacg aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct
    26461 gcggaatttc gtctgctgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag
    26521 catttgctca atcatcttta tgacgactac cgcgtagtaa ccgaccgcac catcgacagc
    26581 cacattaaaa acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc
    26641 gccgtttacg gcgttggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag
    26701 cgacatgact ttgttagccg gagatggcgt tcagaccaga tccggcaaca ttatcccacg
    26761 catggtcagc aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac
    26821 tttaatcggt aaacagcacc tttagatgct gttttcgata cgcaatttca atcaaggagt
    26881 cattatggct ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct
    26941 aaaagcgggc aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc
    27001 ggaaaagggc atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa
    27061 aacggcaagt aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg
    27121 ctccagccag atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc
    27181 caatggcaca agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt
    27241 tggcgtccgg ctttatatcc aaataacgct tagttattaa taattaagag taaccgccgc
    27301 cattaaaata gtgggcactg acgagcactc gttgtgcttt ctttgtttac catcccatac
    27361 ttatcctcct gaacatgttc atgtgccaaa atatcttcta atactttcgt gacgctatcc
    27421 ggtttatgac atacactgat gtaagatttt tacttacagg cgggcgtaaa atggccagaa
    27481 cgatgacggt tgatctcggc gatgagttgc gtgaatttat cgagtcctta attgaatccg
    27541 gcgactatcg cacccaaagc gaagtgatcc gtgagtcact gcgattactt cgagaaaagc
    27601 aagcagagtc tcgcctgcaa gcgttacgtg acatgctcgc agaagggttg agtagtggtg
    27661 aagctcagcc gtgggaaaag gacgcgttct tacggaaggt gaaagcaggg ataaggaaat
    27721 aatgtgcgta ttattaaact catgccaaaa gccaatgagg atttagaagg catctggtat
    27781 tacagctatc accatttcgg cgaaccacaa gccgacagat atgtggaaca tctttcagac
    27841 gttctccaga ttctgagtaa caataatata ggtacgccca gacctgaatt aggcgaaggt
    27901 atttttgttc ttccttttga acgccacgtc atttatttcc tgcaatcacc aggggaaatt
    27961 atcgtcattc gtatccttaa ccaaaaccag gacgccacca gacatttaca ctggagctaa
    28021 acccgccgat tgcgctacaa tgcccgccct taaagtgggg gcactcccct aaccgcttca
    28081 tcaggtgaag cggatctgac ctgtcatcag aacgagagaa ttatgtttaa accggaactc
    28141 ctttccccgg cgggaacgct gaaaaatatg cgttacgctt tcgcttatgg cgcagatgct
    28201 gtttatgcgg gccagccgcg ttattccctg cgtgtgcgca acaacgaatt caaccacgaa
    28261 aatcttcagc tcggcatcaa tgaagcccac gcgctgggga aaaagtttta tgtcgtggtc
    28321 aacattgcac cgcacaacgc caagctgaaa acctttatcc gtgacctgaa accggtggtg
    28381 gaaatggggc cggatgcgct gattatgtcc gatccagggc tgattatgct ggtgcgtgag
    28441 cacttccctg aaatgccgat ccacctttcg gtgcaggcta acgccgtgaa ctgggcgacg
    28501 gtgaaattct ggcagcaaat gggcctgacc cgcgtgatcc tctctcgcga gctgtcgctg
    28561 gaagagattg aagagatccg caatcaggtg ccggatatgg agatcgagat cttcgttcac
    28621 ggcgcgctgt gcatggccta ctccggtcgc tgcctgctct ctggctatat caacaagcgc
    28681 gacccgaacc agggcacctg caccaacgcc tgccgctggg agtacaacgt ccaggaaggg
    28741 aaagaagatg atgttggcaa catcgtacac aagtacgagc cgattccggt gcaaaatgtt
    28801 gagccgacgc tgggtatcgg cgcaccaacc gacaaagtgt ttatgatcga agaggcccag
    28861 cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg gcacttacat catgaactcg
    28921 aaagatctgc gcgccatcgc ccatgtagaa cgcctgacca aaatgggcgt gcattcgctg
    28981 aaaatcgaag gtcgtaccaa atctttctac tattgtgcac gcaccgcaca ggtttaccgc
    29041 aaagctatcg atgacgccgc tgcgggaaaa ccgttcgata ccagcctgct ggaaactctg
    29101 gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc gtcgtcatac tcacgacgat
    29161 tatcagaact acgaatacgg ttattcagtt tctgaccgcc agcagtttgt tggtgagttt
    29221 accggtgagc gcaaggggga cctcgcggcg gtagcggtga aaaataaatt ctccgttggc
    29281 gacagccttg agctgatgac gccgcaaggc aacattaatt ttacccttga gcacatggaa
    29341 aacgccaaag gcgaagctat gccgatagca ccaggcgatg gttatactgt gtggctcccg
    29401 gtcccgcagg atcttgagct caattacgcg ctgctgatgc gtaatttctc cggggaaacc
    29461 acgcgtaatc cccacggtaa gtgattaatt tcgattattt ttcccggatg gaaaattctt
    29521 agaaaccgat cacatacagc tgcatttatt aaggttatca tccgtttcgc tgaaaaacat
    29581 aacccataaa atgctagctg taccaggaac cacctcctta gcctgtgtaa tctcccttac
    29641 acgggcttat tttttatgca taagccctat ccctggtcac cgtcttccat tgaccacatc
    29701 gatagaatct cccttcatag cacgatgcct ttcacgtaac ggcatcgtgc tcgcacaggt
    29761 tccggctaag cacaaccaga acgcgcatgt ttgacgctta ccaaaaaata ttctcactct
    29821 ccacatttga atgtcagacg agcgacgcca tgtaatcctg caccttctgt cttcaggtca
    29881 actatctgca tttttttgcc ctgagtaaca cagaaatggg ctgcatcatt ttttactata
    29941 ttttctgcac cagatattct gcccctggct aaagaagctt cggcttcggt gtagtattgg
    30001 ttatcgagtt tacgctgaat attactttta tatgcaagac caaatttacc gatacttgtc
    30061 tcatcattat gcacagcaca accagacata ataaaaatac taattaatga tatagcagct
    30121 atctttttca tctcaccttc ccccattaaa taccaacgac actctctagt gtttaaatat
    30181 aataatggca tgattattat aattgaatag gattataata aatgttctgt acaacatttc
    30241 ctacataagt aggaattacg gacattgagg cccttcaggg t
//
LOCUS       DAZFRJ010000041        30230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000041 DAZFRJ010000000
VERSION     DAZFRJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.041"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKUN_001635"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKUN_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO6659920.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKUN_001636"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKUN_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6659921.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKUN_001637"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKUN_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO6659922.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHKUN_001638"
     CDS             complement(1087..1581)
                     /locus_tag="ACHKUN_001638"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO6659923.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKUN_001639"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKUN_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO6659924.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKUN_001640"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKUN_001640"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO6659925.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKUN_001641"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKUN_001641"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO6659926.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKUN_001642"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKUN_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO6659927.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKUN_001643"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKUN_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO6659928.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKUN_001644"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKUN_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO6659929.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKUN_001645"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKUN_001645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO6659930.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHKUN_001646"
     CDS             complement(9275..9769)
                     /locus_tag="ACHKUN_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO6659931.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKUN_001647"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKUN_001647"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6659932.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKUN_001648"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKUN_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO6659933.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKUN_001649"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKUN_001649"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6659934.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHKUN_001650"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHKUN_001650"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO6659935.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKUN_001651"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKUN_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO6659936.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKUN_001652"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKUN_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO6659937.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKUN_001653"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKUN_001653"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO6659938.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKUN_001654"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKUN_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO6659939.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHKUN_001655"
     CDS             17948..18874
                     /locus_tag="ACHKUN_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO6659940.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKUN_001656"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKUN_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO6659941.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHKUN_001657"
     CDS             complement(19225..21444)
                     /locus_tag="ACHKUN_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO6659942.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKUN_001658"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKUN_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO6659943.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKUN_001659"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKUN_001659"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO6659944.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKUN_001660"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKUN_001660"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO6659945.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHKUN_001661"
     CDS             complement(26816..27298)
                     /locus_tag="ACHKUN_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO6659946.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKUN_001662"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKUN_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO6659947.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKUN_001663"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKUN_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO6659948.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKUN_001664"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKUN_001664"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO6659949.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..>30230)
                     /locus_tag="ACHKUN_001665"
     CDS             complement(29946..>30230)
                     /locus_tag="ACHKUN_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO6659950.1"
                     /translation="MLTYWLIDAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIA
                     AFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7465 a         8079 c         7633 g         7053 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg ttaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat
//
LOCUS       DAZFRJ010000042        29253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000042 DAZFRJ010000000
VERSION     DAZFRJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.042"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..920
                     /gene="ybhD"
                     /locus_tag="ACHKUN_001666"
     CDS             3..920
                     /gene="ybhD"
                     /locus_tag="ACHKUN_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6659951.1"
                     /translation="MLAESSSFNNAAKLLNITQPALTRRIKKMEEDLHIQLFERTTRK
                     VTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPTAVFYFLPLAIGK
                     FNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFTPLVNEPFVLACR
                     RDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLDWFYEVRHLSTSL
                     GLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKDAVLSPAAERFFA
                     LLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(961..1956)
                     /gene="pgl"
                     /locus_tag="ACHKUN_001667"
     CDS             complement(961..1956)
                     /gene="pgl"
                     /locus_tag="ACHKUN_001667"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO6659952.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2111..2929
                     /gene="ybhA"
                     /locus_tag="ACHKUN_001668"
     CDS             2111..2929
                     /gene="ybhA"
                     /locus_tag="ACHKUN_001668"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO6659953.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(2930..3988)
                     /gene="modC"
                     /locus_tag="ACHKUN_001669"
     CDS             complement(2930..3988)
                     /gene="modC"
                     /locus_tag="ACHKUN_001669"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO6659954.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(3991..4680)
                     /gene="modB"
                     /locus_tag="ACHKUN_001670"
     CDS             complement(3991..4680)
                     /gene="modB"
                     /locus_tag="ACHKUN_001670"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO6659955.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4680..5453)
                     /gene="modA"
                     /locus_tag="ACHKUN_001671"
     CDS             complement(4680..5453)
                     /gene="modA"
                     /locus_tag="ACHKUN_001671"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6659956.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5619..5768)
                     /gene="acrZ"
                     /locus_tag="ACHKUN_001672"
     CDS             complement(5619..5768)
                     /gene="acrZ"
                     /locus_tag="ACHKUN_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO6659957.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5897..6685
                     /gene="modE"
                     /locus_tag="ACHKUN_001673"
     CDS             5897..6685
                     /gene="modE"
                     /locus_tag="ACHKUN_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO6659958.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6753..8225
                     /gene="modF"
                     /locus_tag="ACHKUN_001674"
     CDS             6753..8225
                     /gene="modF"
                     /locus_tag="ACHKUN_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO6659959.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8486..9502
                     /gene="galE"
                     /locus_tag="ACHKUN_001675"
     CDS             8486..9502
                     /gene="galE"
                     /locus_tag="ACHKUN_001675"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO6659960.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9512..10558
                     /gene="galT"
                     /locus_tag="ACHKUN_001676"
     CDS             9512..10558
                     /gene="galT"
                     /locus_tag="ACHKUN_001676"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO6659961.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10562..11710
                     /gene="galK"
                     /locus_tag="ACHKUN_001677"
     CDS             10562..11710
                     /gene="galK"
                     /locus_tag="ACHKUN_001677"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO6659962.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11704..12744
                     /gene="galM"
                     /locus_tag="ACHKUN_001678"
     CDS             11704..12744
                     /gene="galM"
                     /locus_tag="ACHKUN_001678"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO6659963.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            12947..13699
                     /gene="gpmA"
                     /locus_tag="ACHKUN_001679"
     CDS             12947..13699
                     /gene="gpmA"
                     /locus_tag="ACHKUN_001679"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO6659964.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13858..14910)
                     /gene="aroG"
                     /locus_tag="ACHKUN_001680"
     CDS             complement(13858..14910)
                     /gene="aroG"
                     /locus_tag="ACHKUN_001680"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO6659965.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15226..15606
                     /gene="ybgS"
                     /locus_tag="ACHKUN_001681"
     CDS             15226..15606
                     /gene="ybgS"
                     /locus_tag="ACHKUN_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO6659966.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15720..16661
                     /gene="zitB"
                     /locus_tag="ACHKUN_001682"
     CDS             15720..16661
                     /gene="zitB"
                     /locus_tag="ACHKUN_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO6659967.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16658..17377)
                     /gene="pnuC"
                     /locus_tag="ACHKUN_001683"
     CDS             complement(16658..17377)
                     /gene="pnuC"
                     /locus_tag="ACHKUN_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO6659968.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17415..18458)
                     /gene="nadA"
                     /locus_tag="ACHKUN_001684"
     CDS             complement(17415..18458)
                     /gene="nadA"
                     /locus_tag="ACHKUN_001684"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO6659969.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18707..18831)
                     /locus_tag="ACHKUN_001685"
     ncRNA           complement(18707..18831)
                     /ncRNA_class="other"
                     /locus_tag="ACHKUN_001685"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18863..18938)
                     /locus_tag="ACHKUN_001686"
     tRNA            complement(18863..18938)
                     /locus_tag="ACHKUN_001686"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18903..18905),aa:Lys,seq:ttt)
     gene            complement(18972..19047)
                     /locus_tag="ACHKUN_001687"
     tRNA            complement(18972..19047)
                     /locus_tag="ACHKUN_001687"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19012..19014),aa:Lys,seq:ttt)
     gene            complement(19194..19269)
                     /locus_tag="ACHKUN_001688"
     tRNA            complement(19194..19269)
                     /locus_tag="ACHKUN_001688"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19234..19236),aa:Lys,seq:ttt)
     gene            complement(19273..19348)
                     /locus_tag="ACHKUN_001689"
     tRNA            complement(19273..19348)
                     /locus_tag="ACHKUN_001689"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19313..19315),aa:Val,seq:tac)
     gene            complement(19498..19573)
                     /locus_tag="ACHKUN_001690"
     tRNA            complement(19498..19573)
                     /locus_tag="ACHKUN_001690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19538..19540),aa:Lys,seq:ttt)
     gene            complement(19576..19651)
                     /locus_tag="ACHKUN_001691"
     tRNA            complement(19576..19651)
                     /locus_tag="ACHKUN_001691"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19616..19618),aa:Val,seq:tac)
     gene            complement(19787..19862)
                     /locus_tag="ACHKUN_001692"
     tRNA            complement(19787..19862)
                     /locus_tag="ACHKUN_001692"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19827..19829),aa:Lys,seq:ttt)
     gene            complement(20027..20818)
                     /gene="cpoB"
                     /locus_tag="ACHKUN_001693"
     CDS             complement(20027..20818)
                     /gene="cpoB"
                     /locus_tag="ACHKUN_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO6659970.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20828..21349)
                     /gene="pal"
                     /locus_tag="ACHKUN_001694"
     CDS             complement(20828..21349)
                     /gene="pal"
                     /locus_tag="ACHKUN_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO6659971.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21384..22676)
                     /gene="tolB"
                     /locus_tag="ACHKUN_001695"
     CDS             complement(21384..22676)
                     /gene="tolB"
                     /locus_tag="ACHKUN_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO6659972.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22809..24074)
                     /gene="tolA"
                     /locus_tag="ACHKUN_001696"
     CDS             complement(22809..24074)
                     /gene="tolA"
                     /locus_tag="ACHKUN_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO6659973.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(24139..24567)
                     /gene="tolR"
                     /locus_tag="ACHKUN_001697"
     CDS             complement(24139..24567)
                     /gene="tolR"
                     /locus_tag="ACHKUN_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO6659974.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24571..25263)
                     /gene="tolQ"
                     /locus_tag="ACHKUN_001698"
     CDS             complement(24571..25263)
                     /gene="tolQ"
                     /locus_tag="ACHKUN_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO6659975.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25260..25664)
                     /gene="ybgC"
                     /locus_tag="ACHKUN_001699"
     CDS             complement(25260..25664)
                     /gene="ybgC"
                     /locus_tag="ACHKUN_001699"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6659976.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25814..26107)
                     /gene="ybgE"
                     /locus_tag="ACHKUN_001700"
     CDS             complement(25814..26107)
                     /gene="ybgE"
                     /locus_tag="ACHKUN_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO6659977.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(26107..26220)
                     /gene="cydX"
                     /locus_tag="ACHKUN_001701"
     CDS             complement(26107..26220)
                     /gene="cydX"
                     /locus_tag="ACHKUN_001701"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO6659978.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26235..27374)
                     /gene="cydB"
                     /locus_tag="ACHKUN_001702"
     CDS             complement(26235..27374)
                     /gene="cydB"
                     /locus_tag="ACHKUN_001702"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6659979.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27390..28958)
                     /gene="cydA"
                     /locus_tag="ACHKUN_001703"
     CDS             complement(27390..28958)
                     /gene="cydA"
                     /locus_tag="ACHKUN_001703"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO6659980.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7259 a         7754 c         7218 g         7022 t
ORIGIN      
        1 tcatactggc agagtccagt tcatttaaca atgctgctaa attactcaat attacgcaac
       61 ctgcattaac gcgaagaata aaaaagatgg aggaagattt acatattcaa ctatttgagc
      121 gtacaactcg caaggttacg ttaacaaaag caggaaaaag gttgctcccg gaggcgcggg
      181 aattaataaa gaaattcgat gaaacgcttt ttaatattcg tgatatgaat gcttatcatc
      241 gtggtatggt gacgttagcg tgtattccga ccgcagtatt ttatttttta ccgctggcaa
      301 tcggtaaatt taatgagctg tatcccaata ttaaagtgcg gattctggaa caaggcacga
      361 ataattgcat ggaatcggtg ctgtgtaacg aatcagactt tggcataaac atgaacaacg
      421 tcacaaattc atccattgat tttaccccac tggtcaacga gccattcgtg ctggcctgtc
      481 ggcgtgacca tccattggcc aaaaagcagc ttgtagaatg gcaggagtta gtgggttaca
      541 aaatgattgg cgtacgttcc tccagcggca accgattgtt gatagaacaa cagctagccg
      601 acaagccctg gaagctggac tggttttacg aagtgcgtca tctttcgacg tcattaggac
      661 tggtagaggc gggactgggg atttcggcgc ttcctggtct ggcaatgccc catgcgccct
      721 attcttcgat tattggtatt ccactggtgg aacccgttat acgtcggaca ttggggatta
      781 ttcgccgcaa agatgccgtg ctttctccgg cagcagaacg cttttttgcc ttgctgatta
      841 atttgtggac tgacgataaa gacaatttgt ggaccaatat cgtcgaacgc cagagacatg
      901 cgttacagga gataggttaa tacaccggcg caggagagac tgcgccggtt aaatcagcgg
      961 ttagtgtgcg ttaaccacca cccacattgg tccctgcccg accgcataac ggcctttttc
     1021 atgcagtagt ccctgctcgc caacaatttc gtataccgag atgtggtgag atttttgccc
     1081 ggcggcaatc agatacttgc cgctgtgatc aacattgaag ccgcgcggct gggtttccgt
     1141 tggctggaag ccttctttac tcaacacgct gccatcttcc gaaacgctga aaacggtaat
     1201 caggctggcg gtacggtcgc aggcgtataa atggcgacca tccggggtga tatgaatatc
     1261 agccgcccaa cgggtgtcgg agaagttttc cggcatcata tccagcgtct ggacacattc
     1321 gatattaccg tgcggatctt tcagttccca gacatccact gagctgttta actcattgac
     1381 gcaatacgca tattgttcgt ttggatggaa taccatatga cgcgggccgg ccccttcaac
     1441 ggtggtcact tccgcagggt cctgcgccac gagatgacca tcatcgctga ccgtaaacag
     1501 gcaaatgcga tcctgcttta atgccggaac ccacagcgta cggttgtccg gtgagatatt
     1561 ggcggaatgg caaccgtcca gcccctcgac cacatcgacg acgcccactg gcaggccatc
     1621 ttccagacgc gttacgctca cgttacccgc attgtaagaa cctacaaaga caaactgccc
     1681 ctggtgatcg gtggaaatat gcgtcggact acccggcagc gcagactctg cggcaaaggt
     1741 cagtgcgcca tcgtccgggg cgatactata cgccaggacg cgaaactcag ggcgaacacc
     1801 aacatagaga taacgtttgt ccgggctgac caccatcggc tgcacctgcc ccggcacatc
     1861 gacaacctgt gtcagcgtca gtgcgccttc atgattcagg ttccagacgt gaatttgctg
     1921 gctctcaggg ctggcgatat aaactgtttg cttcatgaat gctcctttgc atttagcaac
     1981 tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat
     2041 tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct
     2101 ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc
     2161 gaaaaagacc ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta
     2221 tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct
     2281 ggcgctggat acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa
     2341 aaccgtgctg gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatgct
     2401 gaatgaacac cacattcacg gtctgatgta tgtggatgat gcaatggtct atgagcaccc
     2461 gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc
     2521 gactttcaca caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa
     2581 gttcgccctg acgcacgatg acctgccgca attgcagcat tttggtaagc atgtcgaaca
     2641 tgaactggga ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg
     2701 caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa
     2761 cgtcgtggcg ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg
     2821 cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga
     2881 taacaccacc gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta
     2941 tcgacacact tttaatttgc gcgtacagcc acaggccagg tttgatcacc agttcatccc
     3001 tggcccacgg gctgatacgc gcccacagcg ttttacctcc gacttccagt tccacttcca
     3061 cctggccgtt gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg
     3121 tttgctgcgg cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg
     3181 cagcttgcag cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg
     3241 cagtcatcgc gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt
     3301 gctctttcgg cagccacgga ttcatcacgc tactacccca gacttcttcc agtgcgccaa
     3361 aggctttcac ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat
     3421 ccagcgaatg gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat
     3481 aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca
     3541 gcaattctgg tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg
     3601 acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat
     3661 cgaactgatc gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat
     3721 gcggaaacag ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc
     3781 agataccttt ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg
     3841 ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa
     3901 agatagcagt gatgccattg gcgggcagcg tttcattaat ggtcaggcaa tggttgccca
     3961 aggtctggga aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct
     4021 ggccagccat tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag
     4081 tctcgccgcg ccactttccc cgccgggggt ctggatcagg gtatacatgg cagaaggaat
     4141 ggttcgcgtt tcaccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag
     4201 agaacgagca aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt
     4261 gatagtaaag aaaacgcgcc agcgcccggc ccccagtgtt cttgcggcct gttcgagttt
     4321 gacatcaacc ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac
     4381 ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata
     4441 cagacgttca ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac
     4501 cacgggcggt aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa
     4561 cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc
     4621 cagggaagaa actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat
     4681 tacttggttg taaatccgta acgtttaaag atttccgcag cctgcggccc tttcaaataa
     4741 tcataaaatg ctttcactgt cgcattgtta tgctcttcca caacagcaac cggatattcc
     4801 acttttttat gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctggcaact
     4861 gcgtcagaac cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg
     4921 ccacgaacat cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt
     4981 tgcagtgctt ctttcgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg
     5041 cgaccgccat tcagcagcga agtccagttg gttttgctgt cgatggtgaa atctttctgc
     5101 tcgctggctt tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc
     5161 gtatcgatcg cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac
     5221 agatccgcag gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa
     5281 gaaaccacat ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta
     5341 gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca
     5401 acagcgaaag agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac
     5461 cccttaatgt aacgttatgt aggcgacaat ataacggcta gctcagaatt ttcccagcag
     5521 ttattgctaa cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg
     5581 agaaaaagaa atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg
     5641 tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc
     5701 aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac
     5761 tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa
     5821 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
     5881 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
     5941 attcgccgac ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag
     6001 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
     6061 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggcgg
     6121 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
     6181 ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct
     6241 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggcaccat
     6301 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
     6361 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgcg cgtctggggc tggatgaagg
     6421 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggt attactcagg acgaggcggt
     6481 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
     6541 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
     6601 aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
     6661 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
     6721 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
     6781 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     6841 atagttgggc gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg
     6901 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     6961 tctccttcga gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata
     7021 tgctcggccc tggcgaagat gacaccggac gcactacggc tgagatcatt caggatgaag
     7081 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     7141 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     7201 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     7261 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac
     7321 tggtgctcaa tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg
     7381 attgcacgtt agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc
     7441 aactggcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     7501 cacgtcacgc cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt
     7561 ataacgatcg ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc
     7621 aaattgtcgg gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc
     7681 atccgcaagg ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa
     7741 ccatctggga tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     7801 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     7861 atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     7921 ttgataaacg tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     7981 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     8041 aggggcttga tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg
     8101 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     8161 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     8221 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt
     8281 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     8341 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     8401 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     8461 taccataagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     8521 tggaagtcat acctgtgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     8581 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     8641 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca
     8701 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa
     8761 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     8821 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     8881 caaaattcca tacgttgaaa gcttcccgac cggcacacca caaagccctt acggcaaaag
     8941 caagctgatg gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg actggagcat
     9001 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     9061 tccgcaaggc attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg
     9121 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     9181 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     9241 caagccaggc gtacacatct acaacctcgg cgctggcgta ggcaacagcg tgctggacgt
     9301 ggttaatgcc ttcagcaaag cctgcggcaa accggttaat tatcattttg caccgcgtcg
     9361 cgagggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     9421 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     9481 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc
     9541 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     9601 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     9661 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     9721 caggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     9781 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     9841 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     9901 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     9961 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
    10021 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
    10081 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
    10141 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
    10201 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
    10261 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
    10321 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
    10381 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
    10441 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
    10501 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
    10561 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
    10621 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
    10681 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg cgccacgcga
    10741 tgaccgtaaa gttcgcgtaa tggcagccga ttatgaaaat cagctcgacg agttttccct
    10801 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
    10861 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
    10921 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
    10981 attgcagcag ctttattatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
    11041 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
    11101 cggcaagaaa gatcatgcct tgctgattga ctgtcgctca ctggggacca aagcagtttc
    11161 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
    11221 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
    11281 agccctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
    11341 cgtggcgaaa cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
    11401 cgcgctggag cagggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
    11461 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
    11521 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
    11581 cgtcgcgttg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
    11641 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
    11701 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
    11761 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca
    11821 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg
    11881 gaatgctatc aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc
    11941 atcgccaata gccgttatac ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc
    12001 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
    12061 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
    12121 ggtaatctcg gcgcaacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
    12181 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
    12241 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
    12301 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
    12361 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
    12421 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
    12481 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
    12541 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
    12601 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
    12661 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
    12721 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
    12781 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca
    12841 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
    12901 ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa
    12961 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
    13021 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
    13081 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
    13141 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
    13201 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcag aaaccgctga
    13261 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtca ctccgccgga
    13321 actgactaaa gatgatgagc gttatccggg tcacgatcca cgttacgcga agctgagcga
    13381 gaaagaactg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
    13441 gaatgaaact attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg
    13501 taactcttta cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga
    13561 gctgaatatc ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
    13621 gaaacgctat tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa
    13681 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag
    13741 ccggcttttt tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc
    13801 aggcctgtgg tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta
    13861 cccgcgacgc gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca
    13921 gccgatgcag gcatcggtga tgctcttacc gtaggccagc ggctccccgc tctcgaggct
    13981 ctgattgcct tccaccagat ggctttccac catcacgcca ataatggctg tttcgccacc
    14041 ggcaatctgc tggcaaacgt cagcacacac atccatctgc tttttgaatt gtttggacga
    14101 gttagcatgg ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc
    14161 tttcacttca gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat
    14221 atggcaatca ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga
    14281 caggaagcag tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc
    14341 gtctgtgcca tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac
    14401 ctgcgattcg gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatattg
    14461 tggggtgatc atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag
    14521 cagcaattta cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg
    14581 atcgttaatc agccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat
    14641 tacgatttcc agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc
    14701 ttttgctgcg acaggatcat gaattgagca tgggccaatc accaccaaca ggcgatcatc
    14761 attacctttc aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt
    14821 ttcagtagcg gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt
    14881 gatgcgtaaa tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta
    14941 gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact
    15001 atgaacggat taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat
    15061 ctctcctaca atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct
    15121 gcgtttcata acctggctga aatcttaaac caatgcctta tattcacctg caaatgcact
    15181 gttggaagag gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa
    15241 ctggccacat tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc
    15301 gatagcggag cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta
    15361 gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc
    15421 ggttctggtg gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc
    15481 aaagatgaag agcacaagaa caccatgtgc aaagatggtc gctgtccgga tatcaataaa
    15541 aaggtacaaa ccggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg
    15601 cagtaaccct gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca
    15661 cttctaaaag cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca
    15721 tggcgcactc acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct
    15781 tgttgtatgc tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc
    15841 tttctggttc tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc
    15901 tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt
    15961 tcggttggct aagactgacc acgctcgcgg cgtttgtgaa tgccatagcc ctcgtggtaa
    16021 ttaccatttt gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg
    16081 gcggcatgat gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt
    16141 tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ctgcatgtgc
    16201 tcggggacct gctgggttcg gttggggcta ttattgccgc gttgattatt atctggactg
    16261 gctggacacc tgctgacccc attctctcga tactggtgtc gcttctggtt ctgcgtagcg
    16321 cgtggcgact gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg
    16381 atatcgctga actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc
    16441 atgtacatgt atggatggta ggcgagaagc cggtgatgac gttgcatgtg caggtgatcc
    16501 cgccacacga tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc
    16561 agattgagca cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc
    16621 tgaatgaagg ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc
    16681 ctctttcacg tgcgctgttg atccacatcc ggctgccgtt gagcgcaata aaggtcagga
    16741 tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca
    16801 cgttaataat cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg
    16861 ccacgataga taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt
    16921 caggcatcac aacctgtaat cctaatgctt gcatgatcat gactgccagg cgggtcaaaa
    16981 atgcaaacac cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca
    17041 accagctgag tgccttcggc aatggcaacc agcgaatttt caactccgcc tcgttctgac
    17101 tggtttgtcg cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta
    17161 atagcaggct ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga
    17221 tcaggccaaa gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc
    17281 cggcgatcgt gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta
    17341 tatgtaccag gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta
    17401 cgccttatta ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc
    17461 accagcgccc tctctcgcag cctttcatca acatgaacct cgtgattgct tccttcctgt
    17521 tctaatgcct ctgcgatgga ctgaaggcca ttcatggcca tccacggaca atgcgcgcag
    17581 ctgcggcagg ttgcaccctc acctgcggtt ggtgcttcca gtaactcttt atctggcact
    17641 gcctgctgca ttttgtagaa aatgccccga tcggttgcca cgataagccg ctgatgtggc
    17701 aatgttttcg cagcagcgat cagttgactg gtggaaccga ccgcatccgc catatcgaca
    17761 atagcttgtg gtgattctgg atgcaccagt atggcagcat ccgggtattc ttcttgcaag
    17821 cgggttaacg cctgagtctt aaattcatca tgcacaatac aggcaccctg ccagcataga
    17881 atgtctccac ccgtctgttt ttgcacgtaa cgccccagat gtttgtcggg tgcccagatg
    17941 attttttcac ccaaactatc aagatgatca ataagttcga cggcaatgct tgaagttacc
    18001 acccaatctg cgcgcgcttt taccgcagca gaagtgttgg cgtagacgac gacagtacga
    18061 tcgggatggg catcgcaaaa tgcgttaaat tcttcaacag ggcagccgag atccagtgaa
    18121 cattcagcct gaagtgtcgg catcagaatt gttttttccg gactgagaat tttggcggtt
    18181 tctcccataa atctcacccc agcgactaac aaagtagaag cgggatgctt tgcaccgaag
    18241 cgcgccattt ccagagaatc agaaatacag ccaccggttt cttctgccag ttgttgaatt
    18301 tcgggatcgg tatagtagtg ggcaaccatc accgcattac gttcttttag cagacgtttt
    18361 atcttctcgc ggtaatacgc tttttcatca atgcttaacg gcgtcggctt cggggggaaa
    18421 ggataaatcg ccgtgtctgg atcaaacatt acgctcatct taccatctcg ttttacaggc
    18481 ttaacgttaa aaccgacatt agcgtaatat tcgctgttat cggtgtaatt tgttttatat
    18541 gctaaacaag atagtcgaat ggtggggaaa agtcactcga attacgccta attccaccga
    18601 cgtagagttg cttgcacagt aagctctgac gatgtcatca ggtttacaaa gtgaaattga
    18661 ggatgcctga atagcaaaaa agcgccttta gggcgctttt ttacattggt gggtcgtgca
    18721 ggatgactcg cttcgctagc ccttcgggcc gtcgccgcga gcggctccgt tgtttcgctg
    18781 cgctcaactc aaacctgctg caggttcgaa tctttaagta tcgcagatag caaaaaagcg
    18841 cctttagggc gcttttttac attggtgggt cgtgcaggat tcgaacctgc gaccaattga
    18901 ttaaaagtca actgctctac caactgagct aacgacccct tgcgggatta tgctgcttta
    18961 atcattcagg atggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa
    19021 ctgctctacc aactgagcta acgacccata cgggtgttac ctgaaagatt tctactcggg
    19081 gccaatcttt aagattggtg tgtcgtgcag gatgactcgc ttcgcttgcc ctacgggccg
    19141 ttgctggcgc aacgttatcc ttcacgctta acatctgagt ttgatgttaa aattggtggg
    19201 tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta ccaactgagc
    19261 taacgacccg agtggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga
    19321 ggtgctctcc cagctgagct aatcacccga tactgcgctg gatactacaa actacttcca
    19381 ccgaacccac cgtaattagt ggtgggtcgt gcaggatgac tcgcttcgct cgccctacgg
    19441 gccgttgctg acgcaacgtt atcctacacg cttaacatct gtcttcgatg ctaaaactgg
    19501 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
    19561 gagctaacga cccggtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag
    19621 ggagatgctc tcccagctga gctaatcacc caatactacg ctggatacta caactaacta
    19681 tgttagtggt gggtcgtgca ggatgactcg acttcgtctc gcccttcggg ccgttgctga
    19741 tgcaacgtta tccttcacgt tttactaccg cttccacctt agcgattggt gggtcgtgca
    19801 ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac
    19861 ccactttttc gttgctttcg gtttgtttga tatcccgtgg caacggcggc atatattact
    19921 gatttcagac ttgagcgcaa caaaaatttc gatgcagatc actcaactgc ttatgattcg
    19981 cacgacacga ccagaaataa tgcgacttct ggtcgtgtgt tatgcattac atcgcgttca
    20041 gacgtttttg tgcctgttta gcgccatcag taccagggta tttactgata acctgctggt
    20101 atacggcttt cgcttttgcg gtgtcacctt tgtcctgcat gatgacgccg actttaaaca
    20161 tcgcatcagc agcctttggt gacttcggat agtttttcac taccgaagca aaatagtacg
    20221 ccgcatcatc ttttttaccc ttgttgtagt ttaactgacc cagccaataa ttggcgtttg
    20281 gcaggtaagt tgaatcaggg taatttttga tgaaattctg aaatgccacc attgcgtcat
    20341 cctggcggga tttatcctgc accagcgcaa tagctgcatt gtaatccgtg tttgcatcgc
    20401 cgcttttcac cggcgcgcca gcattcgcag taccagcatc agctgtcggc gtcgttgatg
    20461 ccgccgcacc gctttgatcg ctgctggttg attgcgccgc tgcaccaccg ctgctgaggc
    20521 tgtcgatctg caacaggatc tgcttttgcc gctccacgac ctgattcagt tgatactgat
    20581 tttcctgaat ctgaccacgc agggaatcaa tatcggattg attatcagaa agttgttgct
    20641 ggagttgggt taaaagctgg ctgtgagcat tagaaatacg ctcaagttga gtgacgcggt
    20701 cttcgaccga gcctgagccg acactactga ttggtgcctg agcaaaagcg gcccaggggg
    20761 ccgctatacc aaccagtaac gacagactca atagttgatg tctgaagtta ctgctcatgc
    20821 aattctctta gtaaaccagt accgcacgac ggtttttgga gtatgccgct tcgtcatgac
    20881 ccagtactgc aggtttttct ttaccgtaag aaacgatgga gatctggtct gcagaaacgc
    20941 ctttaccctg caggtacatc ttaacggcgt tcgcacgacg ttcacccagg gagatgttgt
    21001 attccggagt accacgttcg tccgcgtgac cttctacggt gactttgtaa gacgggttgc
    21061 tacgcaggaa gtttgcatgt gcatccagca tttgagcgaa gtcagaacgg atatcgtact
    21121 tgtccagatc gaagtaaacg atgttgttct gctgcagctg ttgcatttgc agacgagcct
    21181 gctcttcgga agacatgttg ccgttgccgc cgttcgcatc cataccagtg ccggcaccca
    21241 gcatgccttc gctgccgtca ttgctggcgt tcttgttgga agaacatgcc gcaattgcca
    21301 taacaggcag agcaatcatc agccctttca gcactttgtt cagttgcatt tcaatgattc
    21361 ctttactatt caattaatta ttatcacaga tacggcgacc aggcagggaa tttgacctgt
    21421 ccatcagttg ccggaagacg cgctttgaaa cgcccatctg tagaaaccaa attcagcacg
    21481 gatcccatcc cctgagaaga gctgtagatt accatagtgc cgttaggtgc cagacttggc
    21541 gtttcatcca ggaacgtgga cgacagaact tgtacgcctc ccgttgccag atcttgtttg
    21601 gcaatgtgct gctgcccacc attggagctg accattacca taaatttacc gtcgctgctg
    21661 acatccgcat cctggttctg cgaaccttcc caggtaatac gttgtggcgc accgccgttg
    21721 atattcactt tataaacctg cggacgaccg gcctggtcag aagtaaatgc caggttctgg
    21781 ctatccggga accaggtcgg ttcggtattg ttactgcgac catcagtcac ctggcggatc
    21841 tgaccagaag ccaaatccat tacgtacagg ttcagactac cggttttcga caaggcgaat
    21901 gccagtttgc tgccgtctgg cgagaatgca ggtgcaccgt tgtgacgcgg gaatgaagcc
    21961 acctgacgta cagcgccatt tgccagcgtc tgaataacca gcgcggaacg accgctttcg
    22021 aaggtcacat aagccagttt agaaccgtct ggtgaccacg ccggagacat cagcggctgc
    22081 ggtgaacggt gaacgacaaa ctggttgtaa ccgtcatagt cagatacgcg cagttcatac
    22141 gggaactgac cgccgttggt ctgaacaacg taggcaatac gagtacggaa cgcaccttta
    22201 atgccggtca gtttttcaaa cacttcatca ctggcggtat gaccagcata acgcagccac
    22261 tgcttgttca ctttgtacga gttctgagca agtacagtac ccggtgcgcc gccagtgtca
    22321 acaagttgat aagcaacatt gtaagaacca tccggattcg gagtgacctg accgacaact
    22381 acagcgtcaa tgcccagtgc ggaccatgca gctggttgta cttcctgcgc actacccggc
    22441 tgctgtggca gacgagcgcg atctaacgga ttaaatttac cgctgttacg caagtcagca
    22501 gcaacgatgc cgccaatatc ttcaggtgcc gcaccaggcc ccgcccactg gaaaggaaca
    22561 acaccaatag gacgaccgga atctacaccg ctgtcgatca caatgcggac ttcagcatgc
    22621 agaactgatg cccacagtat gagaaaacca aatgctactc gtaatgcctg cttcatcata
    22681 tctcccttat ctggaccgat ggcccacgat aatttagcag aatgttaaca aactaaaata
    22741 cacaaaacta ccagaacccc gtggcaaccg gtgcctgatg ttgaccgtcc gaacagtcaa
    22801 catcgcgatt acggtttgaa gtccaatggc gcgtttttga acacttcata tactgcctgg
    22861 cttggtggtt tcgggatctt cgcaagttta gctgctgcca acgcagcctg acaaagtgcg
    22921 ggatcgccac cttcaggttt gatatccagt aacataccat cgggtgccag ttttatgcgc
    22981 agcgtacagg ttttgcctgc ataggacgat gcgtcataga acttactttc gatagcagat
    23041 ttaatctgcc cggcatagtt attgatatcg gcccctgatg cgccattgtt tttagtatta
    23101 ccactcccgg caggcgaagc attgttccct ttcgcccctc cccccgtttt cggtgcattc
    23161 ttaccagagc ttagctcacc gaaaatatca tctgcctctg cggccgcttt tgctgcagcg
    23221 gctttttcag ctgcagcttt tgccgctgcc gcttttttgt ctgcggcggc tttttctgcc
    23281 gctgctttct tatcagctgc tgccttttca gcagccgctt tttctgctgc ttttttgtcg
    23341 gctgcagctt tctctgctgc cgctttctta tcagctgcag ccttttcagc agccgctttc
    23401 ttctcagctt ctgctttggc tttttcagca gcttcagttg ccgctttctt tcttgcttca
    23461 gctgcagctg cttctgccgc ttccgctttc ttcttcagtg ccgcagcggc tgcctcggct
    23521 tttttctgcg cttcggctgc ggctttggcg gcttctgctt ctgctttttt ctttgcgtct
    23581 gcagccgctt tcttcgctgc ttcttccgca gctttagcat ctgcttcggc cttcgcttta
    23641 gcatctgccg ccgctttcgc tgccgcctct tcagcttgct tctgctttaa ctcggcctgt
    23701 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    23761 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    23821 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    23881 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    23941 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    24001 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    24061 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    24121 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    24181 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    24241 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    24301 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    24361 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    24421 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca tgaagatcag
    24481 caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact taagttcgcg
    24541 acgacctcgt ccacgcgctc tggccatgac ttacccctta ttgctctcgc taacggtaaa
    24601 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    24661 tttgtttacg cgctggttga ggcggttgta ggccataact gccgggatag cggcaaacag
    24721 accaattgca gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    24781 ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag
    24841 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    24901 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    24961 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaatat
    25021 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    25081 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcgcgcgccg ctgcgttaag
    25141 aatacgggtc cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    25201 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    25261 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    25321 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    25381 cgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    25441 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    25501 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    25561 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    25621 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    25681 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    25741 tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    25801 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
    25861 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
    25921 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
    25981 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
    26041 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaagtc gcgataatct
    26101 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
    26161 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
    26221 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
    26281 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
    26341 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
    26401 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
    26461 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
    26521 atacgtgcag tcaggatggt cagcagcggc agaaccacac ccagtgccgg aatagcccac
    26581 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
    26641 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
    26701 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
    26761 tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg cagataggtt
    26821 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
    26881 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
    26941 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
    27001 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
    27061 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
    27121 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
    27181 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
    27241 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
    27301 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
    27361 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
    27421 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaatttgaac
    27481 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
    27541 aagatgagat cgcctgcggt cagtgacgag ttcgccacag ctgtcggcag cacttcaccg
    27601 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
    27661 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
    27721 cggatgacac tccagaaaga gagcgcgatg attgccagaa gcaggaagcc acacgccacc
    27781 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
    27841 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
    27901 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
    27961 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
    28021 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
    28081 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
    28141 ccgaacagag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
    28201 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
    28261 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
    28321 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
    28381 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
    28441 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
    28501 ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga accgagcgcc
    28561 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
    28621 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
    28681 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
    28741 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
    28801 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
    28861 agcaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
    28921 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
    28981 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
    29041 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
    29101 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
    29161 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa
    29221 attaccgcct ttcagccaat ttgatcgaga aca
//
LOCUS       DAZFRJ010000043        28985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000043 DAZFRJ010000000
VERSION     DAZFRJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.043"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(107..1771)
                     /gene="glnS"
                     /locus_tag="ACHKUN_001704"
     CDS             complement(107..1771)
                     /gene="glnS"
                     /locus_tag="ACHKUN_001704"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO6659981.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1974..3920)
                     /gene="nagE"
                     /locus_tag="ACHKUN_001705"
     CDS             complement(1974..3920)
                     /gene="nagE"
                     /locus_tag="ACHKUN_001705"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO6659982.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4253..5053
                     /gene="nagB"
                     /locus_tag="ACHKUN_001706"
     CDS             4253..5053
                     /gene="nagB"
                     /locus_tag="ACHKUN_001706"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6659983.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5113..6261
                     /gene="nagA"
                     /locus_tag="ACHKUN_001707"
     CDS             5113..6261
                     /gene="nagA"
                     /locus_tag="ACHKUN_001707"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6659984.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6270..7490
                     /gene="nagC"
                     /locus_tag="ACHKUN_001708"
                     /gene_synonym="nagR"
     CDS             6270..7490
                     /gene="nagC"
                     /locus_tag="ACHKUN_001708"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO6659985.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7538..8290
                     /gene="nagD"
                     /locus_tag="ACHKUN_001709"
     CDS             7538..8290
                     /gene="nagD"
                     /locus_tag="ACHKUN_001709"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO6659986.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8547..10211
                     /gene="asnB"
                     /locus_tag="ACHKUN_001710"
     CDS             8547..10211
                     /gene="asnB"
                     /locus_tag="ACHKUN_001710"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO6659987.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10592..10668
                     /locus_tag="ACHKUN_001711"
     tRNA            10592..10668
                     /locus_tag="ACHKUN_001711"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10626..10628,aa:Met,seq:cat)
     gene            10677..10761
                     /locus_tag="ACHKUN_001712"
     tRNA            10677..10761
                     /locus_tag="ACHKUN_001712"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10711..10713,aa:Leu,seq:tag)
     gene            10785..10859
                     /locus_tag="ACHKUN_001713"
     tRNA            10785..10859
                     /locus_tag="ACHKUN_001713"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10817..10819,aa:Gln,seq:ttg)
     gene            10894..10968
                     /locus_tag="ACHKUN_001714"
     tRNA            10894..10968
                     /locus_tag="ACHKUN_001714"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10926..10928,aa:Gln,seq:ttg)
     gene            10984..11060
                     /locus_tag="ACHKUN_001715"
     tRNA            10984..11060
                     /locus_tag="ACHKUN_001715"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11018..11020,aa:Met,seq:cat)
     gene            11109..11183
                     /locus_tag="ACHKUN_001716"
     tRNA            11109..11183
                     /locus_tag="ACHKUN_001716"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11141..11143,aa:Gln,seq:ctg)
     gene            11221..11295
                     /locus_tag="ACHKUN_001717"
     tRNA            11221..11295
                     /locus_tag="ACHKUN_001717"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11253..11255,aa:Gln,seq:ctg)
     gene            complement(11449..12624)
                     /gene="ubiF"
                     /locus_tag="ACHKUN_001718"
     CDS             complement(11449..12624)
                     /gene="ubiF"
                     /locus_tag="ACHKUN_001718"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO6659988.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12770..14194
                     /gene="miaB"
                     /locus_tag="ACHKUN_001719"
     CDS             12770..14194
                     /gene="miaB"
                     /locus_tag="ACHKUN_001719"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO6659989.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14347..15387
                     /locus_tag="ACHKUN_001720"
     CDS             14347..15387
                     /locus_tag="ACHKUN_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO6659990.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15384..15851
                     /gene="ybeY"
                     /locus_tag="ACHKUN_001721"
     CDS             15384..15851
                     /gene="ybeY"
                     /locus_tag="ACHKUN_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO6659991.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15941..16819
                     /gene="corC"
                     /locus_tag="ACHKUN_001722"
     CDS             15941..16819
                     /gene="corC"
                     /locus_tag="ACHKUN_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO6659992.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16844..18382
                     /gene="lnt"
                     /locus_tag="ACHKUN_001723"
     CDS             16844..18382
                     /gene="lnt"
                     /locus_tag="ACHKUN_001723"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO6659993.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18460..20337)
                     /locus_tag="ACHKUN_001724"
     CDS             complement(18460..20337)
                     /locus_tag="ACHKUN_001724"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO6659994.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20792..21700
                     /gene="gltI"
                     /locus_tag="ACHKUN_001725"
     CDS             20792..21700
                     /gene="gltI"
                     /locus_tag="ACHKUN_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO6659995.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21870..22610
                     /gene="gltJ"
                     /locus_tag="ACHKUN_001726"
     CDS             21870..22610
                     /gene="gltJ"
                     /locus_tag="ACHKUN_001726"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO6659996.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22610..23284
                     /gene="gltK"
                     /locus_tag="ACHKUN_001727"
     CDS             22610..23284
                     /gene="gltK"
                     /locus_tag="ACHKUN_001727"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO6659997.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23284..24009
                     /gene="gltL"
                     /locus_tag="ACHKUN_001728"
     CDS             23284..24009
                     /gene="gltL"
                     /locus_tag="ACHKUN_001728"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO6659998.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24127..25062
                     /gene="rihA"
                     /locus_tag="ACHKUN_001729"
     CDS             24127..25062
                     /gene="rihA"
                     /locus_tag="ACHKUN_001729"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO6659999.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25146..26816
                     /gene="hscC"
                     /locus_tag="ACHKUN_001730"
     CDS             25146..26816
                     /gene="hscC"
                     /locus_tag="ACHKUN_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO6660000.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26876..28327)
                     /gene="djlC"
                     /locus_tag="ACHKUN_001731"
     CDS             complement(26876..28327)
                     /gene="djlC"
                     /locus_tag="ACHKUN_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO6660001.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28324..>28985)
                     /locus_tag="ACHKUN_001732"
     CDS             complement(28324..>28985)
                     /locus_tag="ACHKUN_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6660002.1"
                     /translation="EILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNRDDLIQTIYQM
                     TDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWD
                     YVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMHYFSAYSLGRLYWQSSQ
                     CEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVSD
                     L"
BASE COUNT         7114 a         7541 c         7333 g         6997 t
ORIGIN      
        1 gcgaagaaaa gcagaaaaaa cgccgcagag tgcggcgttt gggattgttt gctatttctt
       61 gccggatgcg acgtaaatgc ctcacccggc ctggtagaac ttaaaattac tcgcctactt
      121 tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcc gctgtggaat
      181 ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta cccgcaaccg
      241 catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca
      301 ccgacaggaa atcatccgca gcaccagggt tcggcacgct gaacagacgg tcgtacagac
      361 ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct ttgactttac
      421 gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag atggtggtga
      481 tattgccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca ttacgcagac
      541 gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg cggaaatcgg
      601 cgcgatcgat ccaaatctca ccgctaaacg gcacctgacg actccccatt tccggtttgt
      661 tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg ataaccagtt
      721 tcaccggatc gataaccgcc attgcgcgcg gcgcattttc gttgagatct tcacggatgc
      781 aggattccag cgatgccatc tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc
      841 agaactcacg aatagaagca gcagtgtaac cacgacgacg cagaccggaa atggtcggca
      901 tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct
      961 tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc gggtgaacag
     1021 gaatggtgat gttgtccagc acccagtcgt acaggcgacg gttgtcctgg aactcaagcg
     1081 tacacagaga gtgcgtaata ccttccaggg catcgctgat gcagtgggtg aagtcgtaca
     1141 tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta atacggtaca
     1201 gcaccggatc gcgcatcacg ataaacggtg aagccatgtc gattttcgca cgcaggcagg
     1261 ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa
     1321 cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt cagggtgccg cggtattcgc
     1381 ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg
     1441 cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa
     1501 aacctaacca ctctacgtcg tttttgatcg actcaacata ctcgatatct tcttttaccg
     1561 ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt
     1621 tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac
     1681 gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacggataa
     1741 agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta
     1801 taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt taaaattgat
     1861 aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga agatctccac
     1921 cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca ggcataaagc agattacttt
     1981 ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt aatgatcaag
     2041 ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg ggcgttagcg
     2101 ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg cgcaccctct
     2161 tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc catatggacg
     2221 acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt gaagattttc
     2281 acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac cgccacaccg
     2341 tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc cacgacatca
     2401 ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc ctgcggtttt
     2461 gctacaggcg cggcagttgc cggagttgct tcagctgacg cagcggctac cggaccacgg
     2521 gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac aatcacctga
     2581 atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca catcgtatcg
     2641 ttgacgcggg cagagtcagc cacggtcagg cgcagacggg tgatacaggc gtcaattgct
     2701 ttcaggttgt cagtgccgcc aaccgcagca atatagttgg tcgccagttg attgaggcct
     2761 tcttcagtgt tgctgttggc ttcttcagta acgatctcgt cttctttatc ttcacgaccc
     2821 ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta gatagcgaag
     2881 aagacaacac ccataaccag cagcatccag acgttctggc tggcggccgg caggttatac
     2941 atcaacgcgt agtcgatagc ccccgctgag aaagagaagc cagcatggat acccagcagt
     3001 gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag cagcggtgca
     3061 aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc agtaacagca
     3121 acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc gaagtacatc
     3181 gccagcgccg cacccggcag accgaacatc ataatcggga agaagccgga catgaacatc
     3241 cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac cgtggaaaac cgtacccgcc
     3301 gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg atgcagaccg
     3361 gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc gcccgcagaa
     3421 acgatccact cgcctcctgc atggatagcg tgctgtaccg gcggccagac gtaaccaaaa
     3481 atggccgcca gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg tttgccgccg
     3541 aagaaactca ggaagtccgg cagtttaata tcggaccaac ggttataggc tgcgccacca
     3601 accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt gatggtcacc
     3661 atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc acctgcgctg
     3721 tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt atcaaaaatc
     3781 gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc
     3841 aacagtgccg ccaccggcag caccgcgata gggagctgta acgccctacc gagtcgctgg
     3901 aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc tcgtttaaag
     3961 ccgtattttt attttgctgc aaattgtact gccgatgttc tgtaatcaga ttgttagatc
     4021 atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt
     4081 gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaag cagaaaactt
     4141 attttatcat tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc
     4201 ttaaagatgc ctaatccgcc aacggcttac attttactta ttgaggtgaa taatgagact
     4261 gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata tcgtcaatcg
     4321 tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg
     4381 cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt
     4441 taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag agcatccgga
     4501 aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc cagcagaaaa
     4561 catcaacctt ctcaacggca atgccccgga tatcgacgcc gagtgccgcc agtatgaaga
     4621 aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta acgacggtca
     4681 tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca aaaccctgac
     4741 tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc aggtgccaaa
     4801 atatgccctg actgtcggtg ttggtacatt gctggatgcc gaagaagtga tgattctggt
     4861 gctgggtagc cagaaagcac tggcactgca ggctgcagtt gaaggttgcg tgaaccacat
     4921 gtggaccatc agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg atgaaccttc
     4981 caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag cagaaaatat
     5041 caaaggtctg taattgttat ccctgccctg gctccttgct cagggcaata ttttttaaaa
     5101 tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca cgaatttctt
     5161 gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa
     5221 ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc cggttttatc
     5281 gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga agcggtcagc
     5341 gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac taactatctg
     5401 ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag
     5461 tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat
     5521 ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc cgcgctggtc
     5581 gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc ggaaatggtt
     5641 cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc cggtcactcc
     5701 aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt tgccacccat
     5761 ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc
     5821 gacgaagctg acatttattg cggtattatt gctgatggcc tgcatgttga ttacgccaac
     5881 attcgtaacg ctaaacgtct gaaaggcgac aaactgtgtc tggttaccga cgccaccgcg
     5941 ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata ctaccgtaac
     6001 ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat gattgaaggc
     6061 gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg tatggcgacg
     6121 ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg gcacactcgc cgcaggtaaa
     6181 gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat cgttaacggt
     6241 aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct cagataggta
     6301 atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt gaccagtacg
     6361 ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc agcgtaacca
     6421 aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag caggcctcca
     6481 ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac gcaatcggcg
     6541 tacggcttgg tcgtcacgac gccaccatca ccctgtttga tctcagcagc aaagtgctgg
     6601 cagaagaaca ttacccgctg ccggaacgta cccagcaaac gctggaacat gccctgttga
     6661 atgccattgc tcagtttatt gatagctacc agcgcaaact gcgcgagctg atcgcgattt
     6721 cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac atgccgcata
     6781 ttcaggtaga aaactggggg ctggtagaag cactggaaga acgttttaaa gtgacctgtt
     6841 tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt gcaagtcagg
     6901 attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg attatctcta
     6961 acgggcgcat ttttattggc cgcaacggca acgtcggtga aattggccat attcaggtcg
     7021 aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact atcgctgcca
     7081 acgccgctat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag agccgcgtgc
     7141 cgctggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc gatagcctgg
     7201 cctcggaagt gattgagtat gtcggtcgtc atctgggcaa aaccatcgcc attgctatca
     7261 acttatttaa tccgcaaaaa attgttattg ccggtgaaat caccgaagcc gataaagtgc
     7321 tgctccctgc tattgaaagc tgcattaata cccaggcgct gaaggcgttt cgcactaatc
     7381 tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt gcgctggtaa
     7441 aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa tgtgctttta
     7501 tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa atgtaatttg
     7561 cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag cggaattttt
     7621 gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc cttcgcagac
     7681 cgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt
     7741 ttatacctct gcgatggcga ctgccgattt cctgcgtcgc caggaaggca agaaagcgta
     7801 tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca ctattaccga
     7861 tgtgaaccct gattttgtga ttgttggcga aacgcgttcc tacaactggg acatgatgca
     7921 taaagcggcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc cggacaccca
     7981 cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga aaatctccgg
     8041 gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg cattaaacaa
     8101 aatgcaggcg cattcggaag aaacggtgat tgtcggcgat aacttgcgca ccgacattct
     8161 ggcgggcttc caggcgggtc tggagacgat tctggtgctt tctggtgttt catcgctcga
     8221 cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcggtcg ctgaaatcga
     8281 cgttatctga atagcaacca cgtctcaggg cgtggtttct tatttttacc catcattaaa
     8341 atcatcccat tcaataaaac tcgccaaaaa ttatcgatcc atcaataaat acatcaccac
     8401 accctcccct ttttcacaaa tcagcaatca ccattacgtt ttttattttt gcactgccaa
     8461 atcgcttgcg cacggtgctg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
     8521 caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa
     8581 cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg
     8641 gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgcccac gaacgtctgt
     8701 caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa actcatgtgc
     8761 tggcggtaaa cggtgaaatc tacaaccacc aggcactgcg cgccgaatat ggcgatcgtt
     8821 atcagttcca gaccggatct gactgcgaag tgatcctcgc gctgtatcag gaaaaagggc
     8881 cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag
     8941 atgcatatct gattggtcgc gaccatctgg ggatcatccc actgtatatg ggctatgacg
     9001 agcacggtca gctgtatgtg gcctcagaaa tgaaagccct ggtgccagtt tgccgcacga
     9061 ttaaagagtt cccggcgggg agctatttgt ggagtcagga cggcgaaatc cgttcttatt
     9121 atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc
     9181 tgcgtcaggc actggaagat tccgttaaaa gccatctgat gtctgacgtt ccttacggtg
     9241 tgctgctctc gggtggtctg gattcctcaa ttatttccgc gatcaccaag aaatacgccg
     9301 cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcaatta cactcctttg
     9361 ctgtaggtct gccgggttca ccggatctga aagcagccca ggaagtggca aaccatctgg
     9421 gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg
     9481 tgatttacca catcgaaacc tatgatgtga caactattcg cgcttcaaca ccgatgtatt
     9541 taatgtcgcg taagatcaag gcaatgggca ttaaaatggt gctgtccggt gaaggttctg
     9601 atgaagtgtt tggcggttat ctttacttcc acaaagcacc gaatgccaaa gaactgcatg
     9661 aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc gccaacaaag
     9721 cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg
     9781 tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca
     9841 tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg cagaaagagc
     9901 agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gcggcgcagc
     9961 aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac acgccaacct
    10021 caaaagaagc gtatctgtac cgggagatct ttgaagaact gttcccgctt ccgagcgccg
    10081 ctgagtgcgt gcctggcggt ccttccgtcg cgtgttcttc cgctaaagcg attgagtggg
    10141 atgaggcgtt caagaaaatg gacgatccgt ctggtcgtgc ggttggcgtt caccagtcgg
    10201 cgtataagta agaaatgcga aacagccccg gtgaaatccg gggctgtttc agttattgag
    10261 gtgcttgtta attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg
    10321 acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta ttgttgaatt
    10381 ccgaatttca tcctgtacga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa
    10441 acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga
    10501 gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc
    10561 aacgccgata aggtatcgcg aaaaaaaaga tggctacgta gctcagttgg ttagagcaca
    10621 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccatc ttttttgcgg
    10681 gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt
    10741 caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc
    10801 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
    10861 cttcttcgag taagcggttc accgcccggt tattggggta tcgccaagcg gtaaggcacc
    10921 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc gaagaaacaa
    10981 tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc
    11041 gaatcccgtc gtagccacca aattctgaat gtgtcgaata cgttcggcaa attcaaaaac
    11101 caatttgttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg cattccgagg
    11161 ttcgaatcct cgtaccccag ccaatttatt caagacgctt acatcgtaag tgcgcccagt
    11221 tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc
    11281 ctcgtacccc agccacatta aaaaagctcg cttcggcgag ctttttgctt ttctgcgttc
    11341 attcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg cgacgctgcc
    11401 gcgtcttatc cgacctacgg ttggcacgca tccggcaatg ttgtaaggct acaaccctaa
    11461 cgcatatttc agcgcctgac gtttcaacac gccagcacgc tctgccgcca ttaacccgag
    11521 attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa acagatccat
    11581 accactttgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct tgaggacagg
    11641 ataactggcc cacgcttcac cgtagctgcg ggcattcacc agaacatcaa tcagggcatc
    11701 gacatcacga taaccaagat tccccccctg ccccgccagc ggatggatgg tatgcgcggc
    11761 atcgcccacc agcgcaagcc ccggctgcac atactgcaac gcatgtcggc gcgtcagcgg
    11821 aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat gcttcgcgat
    11881 ttccgcctgg agttgcgcca tattcatatt ctgcaactgg cgaatacgta ccggagagtc
    11941 ataccacacc agcgatgccc agttatcaaa caacggcaga aacgcacgcg gtccgtccgg
    12001 ggtaaattgc tgccaggtgc tgtcgccggg atcgttctca cactgtacgc taatcaacat
    12061 acacgactgc gcatactgcc aggcatgaac gccaattccc gccatctgcc gcacctgcga
    12121 atttgcgccg tcagcaccaa tcaccagctt cgcgcgaatc acttcaccgc ccttcagttc
    12181 cagctcctga agatcattat ctcgatgtaa tgcaatcagc gagcctggca cacgtaacgt
    12241 tactttcgga tgcgcttcca gcgcctgcca caatgcctgt tgcaggacgg tgttttccac
    12301 catatagcca agcagcggca gcttaagttc agcggcgtca aacatcacat gcgccgtttc
    12361 ccactcccac gtttccagtc tgcggtaagg atggcaacgc atagcctgta ctgcatccca
    12421 gacccctaac cctttaagca atgataccga tgcggcgctg atcgccgaga tccgcacgtc
    12481 cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg ttaccgaaaa
    12541 tccgtgctgt gccagcccca gcgccagtgc gccgccgacc attcctccgc cgacaatggc
    12601 aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc tttaagttta
    12661 caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc agcaaagcat
    12721 tacactatgc gccctgcatt cctggctact atttcgcaag agcaagtcga tgaccaaaaa
    12781 actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga agatggccga
    12841 tctgctggat gccacccacg gctatcaact gaccgacgtg gcggaagaag cggatgtgct
    12901 gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg
    12961 tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg gtggctgcgt
    13021 ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata ttatttttgg
    13081 gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg accgcagccc
    13141 ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctgccgg aaccgcgcgc
    13201 cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt gcacctactg
    13261 cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tccgacgata ttctgtttga
    13321 gatcgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc agaatgtgaa
    13381 tgcctggcgt ggtgagaact acgacggcac caccggatcg tttgccgatc tgttgcgtct
    13441 ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc cgatcgaatt
    13501 caccgacgat atcatcgaag tgtatcgcga cacgccggag ctggtgagct tcctgcatct
    13561 gccggtacag agcggttccg atcgcattct gaacctgatg ggccgtactc atacggcact
    13621 ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccggatattc agatcagttc
    13681 tgacttcatt gttggcttcc ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct
    13741 gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac gtccgggtac
    13801 accagccgcc gatatggttg atgatgttcc ggaagaagag aagaagcaac gtctgtatat
    13861 tctgcaagaa cgtatcaatc agcaagcgat ggcatggagc cgccggatgc tcggcaccac
    13921 ccagcgtatt ctggtagaag ggacttcgcg taagagcatc atggagcttt ccgggcgtac
    13981 cgaaaataac cgcgtggtca acttcgaggg cacaccggat atgatcggta aattcgtcga
    14041 tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc gtactgaaga
    14101 tgaaatgggt ctgcgcgtgg cagaaacacc ggaatcggtg attgcccgta cccgcaaaga
    14161 aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcccgccgt tccggtgggc
    14221 cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc
    14281 gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcgacatac agataagagg
    14341 aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa cgcgcgtctg
    14401 ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg tctcggcatc
    14461 gagatcaatc gccgcgacaa ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct
    14521 gcggcagaca ttctgcgtag cctgtatgtc gatacagccc cgatgcgcgg tcagattcag
    14581 gatatcgaac cggaacagat ccaccttgcg attaaagaag cgcgagtact ggagcaaagt
    14641 gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaaccaaacg cggcgtaatt
    14701 aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca tgacattacc
    14761 ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc ggcagttgat
    14821 gccctggagc gtcaggagat tcgccgtatt ctgctgactc gtccggcggt cgaagccggt
    14881 gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta tctgcgcccg
    14941 ctgtacgacg cgctgtttga aatgctgggc tttgagaaag ttgagaaact gattgagcgc
    15001 aacgttattg aagtcgcgcc gctggcctat atgcgtggtc gtacgctgaa cgacgcgttt
    15061 atcattctcg atgagagcca gaacaccacc atcgaacaga tgaagatgtt cctgacccgt
    15121 atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga cctgccgcgt
    15181 aatactaaat caggcttacg tcacgccatc gaagtgctgg ccgatgtcga agagatcagc
    15241 tttaacttct tccacagcga agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac
    15301 gcctatgaag cctgggaaga agccgaacaa aaacgaaaag cggcgctggc ggcagaacgc
    15361 aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg
    15421 aagataattc cgggttaccg gaagagagcc agtttcaaac atggctgaat gcggtgatcc
    15481 cgcagtttca ggaagaatcg gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca
    15541 gtctgaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg
    15601 aagtgccgcc tggcatggaa atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg
    15661 ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg catatggtgg
    15721 tgcacggcag tctgcatttg ttaggttacg atcacatcga agatgacgaa gcagaagaaa
    15781 tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg tacattgccg
    15841 agaaagaata atttgccagg catttgactg gcagacgtcc ccgccgttgg tgattaacga
    15901 cggcgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg acaattcaca
    15961 cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca gccaactttt
    16021 ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt ccgggcagaa
    16081 cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca
    16141 acgcgttcgc gacatcatga tcccccgctc ccagatgatt accctgaaac gcaaccagac
    16201 gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc cggtgattag
    16261 cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctac cgtttatgcg
    16321 cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg tcgttcctga
    16381 aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc acatggcgat
    16441 cgttattgac gaattcggtg gggtttccgg tctggtgacc attgaagaca tcctggaact
    16501 gattgttggt gagattgaag acgagtatga cgaagaagat gatatcgact tccgtcagct
    16561 gagtcgtcat acctggaccg tgcgcgcact ggcttccatt gaagacttca acgaagcgtt
    16621 cggcacccac tttagcgatg aagaagtcga cactatcggt ggtctggtga tgcaggcgtt
    16681 tgggcatctt ccggcgcgtg gcgaaactat cgacatcgac ggttaccagt tcaaagtggc
    16741 gatggccgat agtcggcgta ttattcaggt tcatgtcaaa atcccggatg actcacccca
    16801 gccaaagctg gatgaataaa accgaaactg gatagataac tacatggctt ttgcctcatt
    16861 aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct gcggaacgct
    16921 ggccttctct ccttacgatg tctggcctgc ggcgattgtt tcgctgatgg ggcttcaggc
    16981 gctgaccttt aaccgccgcc cgctccagtc tgccgctatt ggcttctgct ggggatttgg
    17041 cctctttggc accggtatta actgggtcta tgtcagcatc gcgacctttg gcgggatgcc
    17101 tggcccggtt aacatcttcc tggtggtgct gctggcggcg tatttgtcgc tgtataccgg
    17161 actgtttgct ggcgtgctgt cgcgcctgtg gccgaaaacc acctggctgc gcgtggcgat
    17221 tgccgcccct gctctctggc aagtgaccga gtttctgcgc ggttgggtgc tgactggctt
    17281 cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc tggcaccaat
    17341 aatgggcgtg gaagccatta acttcctgct gatgatggtt agcggcctgc tggcactggc
    17401 gttagtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg cccttccctt
    17461 cccgctgcgt tacatccaat ggtttacccc gcaaccggag aaaaccattc aggtttcgat
    17521 ggttcagggc gatattccgc aatcactgaa atgggacgga gaccagcttc ttaatacgct
    17581 gaagatttac tacaacgcaa cggcaccgct gctgggcaaa tcatcgttga ttatctggcc
    17641 ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag cactggacgg
    17701 tgagttgcgt gataaaggta gctcgctggt aaccgggatt gtcgacgcgc gtctcaataa
    17761 gcagaaccgc tacgatacct acaacaccat catcacgctg ggtaaaggtg caccgtacag
    17821 ctacgaatca gccgatcgct ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc
    17881 gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt cgtcgttcag
    17941 ccgtgggcca tatatccagc cgccgctgtc ggtaaatggt attgagctta ctgcggccat
    18001 ttgctacgag atcattcttg gcgagcaagt gcgcgataac ttccgcccgg ataccgacta
    18061 tctgctgact atctccaacg atgcgtggtt tggtaagtct attggtccat ggcagcactt
    18121 ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa
    18181 cggcattacg gcggtgattg gcccgcaggg tgagattcag gcgatgatcc cgcagttcac
    18241 ccgcgaggtg ttgaccacta atgtgacacc gaccaccgga ctcacaccgt atgcacgtac
    18301 cggcaactgg ccgctgtggg tgctgacggc gctgtttggt tttgccgctg tgctgatgag
    18361 tctgcgagca cgtaaacgtt gatgtgcctg atgtatcctg catgagcaaa acgtactttc
    18421 tcattgagct ttggggcatg gttatcatgc ccctttttct tacggttcag ggatacaagc
    18481 cacattgcca tattggggat gacgaataat tgcctgcgga tcaagcgagc gcgtcctgtc
    18541 catttggatc aacccgttct tcgccatgac aaatattttt ctaccatccg ctgaaaaacg
    18601 aaggtgatta acgtagctgg caggataaac gccaataatc tgcccggcat taatattgag
    18661 caggatgaca taaccttgca ggccatatga caccaacgct aaatattgac cgtccgggct
    18721 aataccctca acggaataaa cgctcctgct acaccattgc gtcataatgc tggcttgttg
    18781 atcccctacg cttatacgtg aaatcccctg actatcggca aaaataacct cgccgttttc
    18841 aggtcgaaaa cgcatcgccg atatcgttcc acgcgcatca cctgcgggaa agtcttcttt
    18901 ctgatgcgtg atggtattga tccgcacgat gttacctctg cctgctcccg gaaccagtaa
    18961 tgttctttca tccggggaaa gtgccataac ccaggcgcct ggtttaccgc tcgtccccgt
    19021 cccgtcgtat ttctccagcg ggatatccgc cacatgctga taagtcacca gatcaatcgc
    19081 tgacacacta ttactggtgc gattcatcac ccaggctttt gtgccatcac gcgagaggat
    19141 aaaactgtca ggatgtgcac cggtggtaat tgactgaata atctcccctg tcgccacgtt
    19201 aatgaccgat aacgcattcg cttcaaatga cgtcgcataa atcaactttt catccgggct
    19261 taatgccagc gatctgacgc cctgatcttt acagccacca caatgtttga ctgacttttc
    19321 tttcgctatg ggcgctgtaa aggtatgcag gattttcttt tgtccgaggt cgaaaacaac
    19381 aatgctgttg gtgttttcaa ttgcggcata caacgtatta ccatcccgac tgatcgccat
    19441 atcatggata tcggcaatct cagcgagtaa atcaccactg gcctgttcac gacttaccgg
    19501 agcggataat gtttccctga gggcgttgcg ttcctccgct aactgttgtt ttttttgtcg
    19561 tcgctcttgt tcaatctcct gcggataaaa cgcttcacgc aggctttgcc tgacctgtaa
    19621 aactgactca tccatctgct gctgcgcaag ccagatagcc ccggtcagaa gaagactgcc
    19681 cgcaacaata atcccgcctt ccgtaacgag tctgttttgc ccagataaac gcgcgctcag
    19741 ccaacccaac gcgccacccg caatcaggcc gccaatgtgg caagcgttat ctattcctgt
    19801 ttgccgggca ccgtacaaca gtgtcagcgc caccataccc aacagaggaa atactcgccg
    19861 ctggttttta tggtatgtgc ccgtacctga agcaaactgt gttgctatcg ctgcaccagc
    19921 tattcccaga atcgccccgg atgccccaac gctgatgact atctgctcac tttcgcgtaa
    19981 cgcataacag gcgctgatga gcccaccaaa gacgccggag aataaccagg taatcaggag
    20041 tcgccatttc cccagaatct gttccgccac gcctcccacg accaggaatg caaacatatt
    20101 catcagcaga tgagaaacac tactgtgcag aaagatactg ctcaataagc gccagggttc
    20161 acccgacaaa gtaagaggcg caacgtttgc accgagatgg ataagtgcgg attgatcgac
    20221 ctcttcataa ctcatccatt gtgcgagata acaaaatatc ccaatacaga gtgcaaaaag
    20281 gataagggtt attgcgggta attgaacatt taacggctta accgaagatg cagacataag
    20341 gtgtttcttc cttgaaaaga caggtaaaaa gcatttttcc cgtgtgcagt tatcatcccg
    20401 gcaaacgtcc catgccgaaa agataattct gtccgaaata tgactttttc agcctaaaat
    20461 tacaatcgta attaattcgc ccctaagtat tttttggcat gtaagttgct ttatttactg
    20521 tgtaagcgat gaaatcagcc gcgacgcaac caggttgcac caccacaaat cagcggtagc
    20581 accaaaaagg tgcggtttaa gaattttgct tcggaatggt gcggcgcatc tcgcaaaaac
    20641 aaacaataaa acagcaatat ctttacattt agccaaacta aatgttacct aacaggcaca
    20701 acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg gtatccatgc
    20761 gttcttaacg cagaagataa aggagttgga tatgcaatta cgtaaacctg ccacagcaat
    20821 cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc cggtagcggg
    20881 cagtacgctg gacaaaatcg ccaaaaacgg cgtgattgtc gtcggtcacc gtgaatcttc
    20941 agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc aggattactc
    21001 caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc aggtaaaact
    21061 gattccgatt acctcacaaa accgtattcc actgctgcaa aacggcactt tcgattttga
    21121 atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct ctgatactat
    21181 tttcgtggtc ggtacgcgcc tgctgaccaa aaagggcggc gatatcaaag attttgccga
    21241 cctgaaaggc aaagccgtag tcgtcacttc cggcactact tctgaagttt tgctcaacaa
    21301 actgaatgaa gagcaaaaaa tgaatatgcg catcattagc gccaaagatc acggtgactc
    21361 tttccgtacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgatg ccctgctggc
    21421 cggtgaacgt gcgaaagcga agaaaccaga caactgggat atcgtcggca agccgcagtc
    21481 tcaggaagcc tacggttgca tgttgcgtaa agatgatccg cagttcaaaa agctgatgga
    21541 tgacactatc gctcaggtgc agacttccgg tgaagcggaa aaatggtttg ataaatggtt
    21601 caaaaatcca attccgccga aaaacctgaa catgaatttc gaactgtcag acgaaatgaa
    21661 agcactgttc aaagaaccga atgacaaagc actgaactaa ttacaagaac caggggcgga
    21721 aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga tggtcgttcc
    21781 ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag cagttaacgc
    21841 tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg ggtatttttt
    21901 tacaacaagc cccgttcggc aacaccacct atcttggttg gatctggagt ggttttcagg
    21961 tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg
    22021 gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg tatgtcgaac
    22081 tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg atcccggagt
    22141 tgctgccaga gaaaatcggt atgtggttta aggccgagct ggatcccaat attcagtttt
    22201 tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac
    22261 gcgccgccat tcagtcgttg ccgcgtgggc aaaaaaatgc cgcgctggcg atgggcctga
    22321 cgctaccgca ggcttatcga tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc
    22381 cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg actattggtc
    22441 tggtggatat ggcggcgcag gcgggtaaat tgcttgatta ctcagcccac gcctgggagt
    22501 cgtttaccgc tattacgctg gcgtatgtgt tgattaacgc ggttatcatg ctggtaatga
    22561 cgctggttga acgtaaagtt cgcctgcctg gcaacatggg gggcaaataa tgtacgagtt
    22621 tgactggagt tccattgtcc cttccctgcc atatctgctc gacgggctgg tgatcacgct
    22681 gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggcaccatgc tggcggtgat
    22741 gcgcttgtcc agctttgcgc ccgtagcgtg gtttgccaaa gcctacgtta acgtattccg
    22801 ctcgattcct ttagttatgg ttttgctgtg gttttacctg atcgtgccag gttttctgca
    22861 aaacgtgctg ggattatcac caaaaaatga tatccgttta atttcggcga tggtggcgtt
    22921 ttcaatgttt gaagcggcct actattcaga gattatccgt gctggtattc aaagtatttc
    22981 tcgtggtcag tcgagcgccg cgctggcgtt gggaatgact cactggcagt cgatgaaact
    23041 gattattctg ccgcaggcgt tccgcgcgat ggtgccgctt ctgctcactc agggcatcgt
    23101 actgttccag gatacctcac tggtgtatgt gttatctctg gccgatttct tccgtaccgc
    23161 ctcaaccatt ggtgagcgtg atggtacgca ggttgagatg atcctatttg ccggatttgt
    23221 ttatttcgtt attagtctta gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc
    23281 ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt gctgaccgac
    23341 tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc tggttccggc
    23401 aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg tgaaatcacc
    23461 gtcgatggta tcgtggttaa tgacaagaaa accgatctgg caaagctgcg ttcccgcgtc
    23521 gggatggtgt tccagcattt tgagttgttc ccccatttgt cgattattga aaacctgact
    23581 ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa agcattgaaa
    23641 ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca gctttccggc
    23701 ggtcagcagc agcgtgtggc aatcgctcgc gcgttgtgta tggatcctat tgcgatgctg
    23761 ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct ggacgtgatg
    23821 gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat gggctttgcc
    23881 cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga agactcgccg
    23941 aaagacgctt tcttcgatga tccgaaatcg gaccgcgcga aagacttcct cgcgaaaatc
    24001 ctgcattaat cactctggcg cgcataccca agtgcgcgcc atctcacgct ttatcgtttc
    24061 gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac acaacaagga
    24121 gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgacga cgctatcgca
    24181 atagttctcg ccctcgcctc accagagctt gatgtcaaag caattacgtc ttccgccgga
    24241 aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt gcttaatcgc
    24301 accgatattc cggtagcagg cggcgcggta aaaccgttaa tgcgtgagtt gattatcgcg
    24361 gacaatgtgc acggcgaaag cggtctggat ggcccggcat taccggaacc gacgttcgca
    24421 ccgcaaaact gtacggcggt agagctgatg gcgaaaacgc tgcgcgaaag tgcggaacct
    24481 gtcacgattg tgtctaccgg accgcaaact aacgttgcct tgctgctcaa tagccatccg
    24541 gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac
    24601 tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga aattgtcttc
    24661 cagtcaggga tcccagtggt gatggccggt ctggatgtta ctcacaaagc acaaatccac
    24721 gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa
    24781 ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt cggtgcaccg
    24841 ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac cactgttgag
    24901 cgctgggttg gcgtggaaac acaggggaaa tatagccagg gtatgacggt tgttgattat
    24961 tattatctga ctggcaataa accgaatgcc actgtaatgg tcgatgttga tcgtcagggc
    25021 tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacacacgtc attgattaag
    25081 atcaccacgc gttacctggg tggtcttaat ctgtttatct ctttttcttt caagatataa
    25141 atgcaatgga taatgcagaa ctcgccattg gtatcgatct cggcactacc aatagtttaa
    25201 ttgccgtctg gaaagacggt gccgcgcaat taattccaaa taagttcggt gaatatttaa
    25261 caccatccat aattagcatg gatgaaaata atcatatttt agtcggaaaa ccggctgtat
    25321 cacggcgtac ttcgcatccg gataaaacgg cagcgttatt taaacgtgca atgggcagta
    25381 ataccaactg gcggttaggc agcgacacat ttaacgcgcc agaactgtcc tctttggtat
    25441 tacgctcatt aaaagaagat gccgaagaat ttctgcaacg tccgattaaa gatgtggtga
    25501 tctccgttcc ggcttatttc agcgatgaac aacgcaagca tacccgttta gcagcggagt
    25561 tagccgggtt aaatgcggta cgcttaatta atgaacccac agcagctgcg atggcgtatg
    25621 gcctgcatac ccaacaaaat acccgttcgc tggtgtttga tctcggtggc ggcacgtttg
    25681 acgttacggt gcttgagtac gccacgccgg tgattgaagt tcacgcctcc gctggcgaca
    25741 actttcttgg tggcgaagat tttacccata tgctggtcga tgaggtttta aaacgcgcgg
    25801 atgtcgccag gaccacgctt aacgagagtg aactgggagc cttgtacgcc tgtgtggaag
    25861 cggcaaaatg tagcaatcaa tcgccattgc acattcgctg gcagtaccag gatgaaacgc
    25921 gggagtgcga attttacgag aacgaactgg aagatttgtg gttgccgctg ctcaatcgct
    25981 tgcgagtgcc gattgaacag gcgttgcgcg atgcacgtct gaagccgagt caaatcgaca
    26041 gtctggtgct ggttggcggc gcgtcacaaa tgccgctggt gcagcgaatc gccgtgcgtc
    26101 tgtttggcaa attaccgtat caaagttacg acccgagcac cattgtcgcg ctgggcgcag
    26161 caatccaggc cgcctgccgc ttacgcagtg aagatattga agaggtgatc ctcactgata
    26221 tttgccctta ctcgttgggc gttgaagtta accgccaggg cgtttccggc attttctcgc
    26281 caattattga acgaaacacc acagtacccg tgtcgcgtgt agaaacttac tcgaccatgc
    26341 acccggaaca gaattcaatt acggttaacg tctatcaggg agaaaaccac aaagttaaaa
    26401 ataacattct ggttgaatcc ttcgatgtgc cgttgaagaa aaccggggct tatcagtcga
    26461 ttgatattcg ctttagttat gatatcaacg gcttgcttga agttgacgtg cttctggaag
    26521 atggcagcgt taagtccaga gtcattaatc atagcccggt aacattgagc gcgcagcaga
    26581 ttgaagagag tcggacgcgg ttatccgcat tgaaaattta tccgcgcgat atgctcatca
    26641 accgcacctt taaggccaaa ctggaagagt tgtgggcgcg ggcgctgggt gacgagcgag
    26701 aagagattgg ccgggtgatc accgattttg atgcggcgtt gcagtcaaac gatatggccc
    26761 gcgtcgatga ggtcaggcgg cgggcgagcg tttatttagc cattgagacc tcataatcac
    26821 ggctgccgga tgcggcgtaa atgctgcatc cggcatcggc gcaaaaaaca catcgtcaac
    26881 taaacaacat cgcgattttg ccgagagcat aacctataac cagcaccgtt aaactgattt
    26941 tgataatgct ggtaatgctc tgtggttttt caccctcatc atttttttct tcatggtttt
    27001 caggtgttgg ctcatcgaca tcttcatcct ccggctcgag ttttactttc gtcaacagca
    27061 tccgggtgat aatggcctct aatggatgag catctccgcc ttccagcctt gtattaagac
    27121 gggccagcat ctcttgtttc ggaaacgctt caagtactac ctgttccgct acccgataca
    27181 aatccagtaa cggtgaatct tcttctgtct cgctaccgac tctcgatttc caccacgccc
    27241 atgctttgcg tttgaagaat aacgactcaa taaacgattg acctaacggc gataattttc
    27301 cgctatgtaa aatttccgcc cataacagcc gcgtccgggc gctctcacca ggaacatatt
    27361 tcagcggttg accttgcgca tctaccatgc tccgcgcttc gataaccatc actaataacg
    27421 cgaaataatc cgggcaatga cgcttgctcc actgcaacaa caaattatca gcttgtgtag
    27481 atgggtaact ttcccggtac gcgcagagat cggcaagcag gctttccccc tcgccgcagt
    27541 acaacctttg agcacacaag taatatcggg catcttcgct ttctggttct tccatttgcc
    27601 actgtttggc tacaggaatg agttccgcca tacctgtttg caccgagctg taccagcgca
    27661 acagtttgcg atgtaacttt gcatcatcag gaatgaacca cggaccttcc atcgccaaaa
    27721 acgcagtgac gtagtcagga tggtaacggc agcaacgttc cagcgcgaag taatactcaa
    27781 cagtctggtt ctgcaacgca acgggatatt ccagaaggct aaggaaatcg aaacaatcac
    27841 cagcctgaat ggcctcaaga aaagcctccc tttcttcctc atccattcct tcgctgtcat
    27901 taaacgaatg ccagttcaaa cgctctgcca gcaaagaagc acaatttaat gaaagatatc
    27961 gcgcttctat agcgattgca cacagcggcc agcgtaattg atcgacatcc tccatgttcc
    28021 aggtatttaa ttgctgaata aatttctgcc aggcggaggg ctgaaaacga tcactttctg
    28081 aatccagtaa tgtcctgaat gcacgcagta tttcatgttc agcggcggca tctttttctt
    28141 catcatctgc ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca taggcctggc
    28201 gtaatgcttt aaacccctgc ggatcggttt cgggatgaca taacggtaag cgagcaagat
    28261 aagcctggcg gataatgtct atttgcgtcg tgctttcaat ttcgaggatt tgccaacatg
    28321 ttttcatagg tcgctcacat ccttaagcgt taccgggcat tcaggctgga cagttaatgg
    28381 ctgccaggga agcgtggcat agaaacgatc gcttcccgct gccagctctt cgaacatccg
    28441 actcccggcg ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag tgttgtcttc
    28501 gcactgagaa gattgccagt agagacgtcc taacgaatag gcggaaaaat agtgcatcca
    28561 gctgtgatag taatggtgag ctcgtacata gacgcacgat tgcagccata aactctcttc
    28621 ttccgtcagc cagttgttga gcacaccgac ccgtgacaga aaccccattc gaacataatc
    28681 ccaggctttg attccccctt ctccacagca catagcggtt tgggcaacga attgggtata
    28741 aatcaaccct ctctccgagc caccagcaat taatgctttc cactcttcag gggaagagcg
    28801 atgccatgtc agatataagc cagctaaatc attggcatga ccatcatcag tcatctgata
    28861 aatagtctga attaaatcat cacgattatt tattccccag gatgatgaga gatcgaattc
    28921 ttttatcccg gtatacattt ttggcgagtc atgcgcgggt ttgcaaccct gattaagtat
    28981 ttcca
//
LOCUS       DAZFRJ010000044        28911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000044 DAZFRJ010000000
VERSION     DAZFRJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.044"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            103..1380
                     /gene="waaA"
                     /locus_tag="ACHKUN_001733"
     CDS             103..1380
                     /gene="waaA"
                     /locus_tag="ACHKUN_001733"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO6660003.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1388..1867
                     /gene="coaD"
                     /locus_tag="ACHKUN_001734"
     CDS             1388..1867
                     /gene="coaD"
                     /locus_tag="ACHKUN_001734"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO6660004.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1906..2715)
                     /gene="mutM"
                     /locus_tag="ACHKUN_001735"
     CDS             complement(1906..2715)
                     /gene="mutM"
                     /locus_tag="ACHKUN_001735"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO6660005.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2813..2980)
                     /gene="rpmG"
                     /locus_tag="ACHKUN_001736"
     CDS             complement(2813..2980)
                     /gene="rpmG"
                     /locus_tag="ACHKUN_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO6660006.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(3001..3237)
                     /gene="rpmB"
                     /locus_tag="ACHKUN_001737"
     CDS             complement(3001..3237)
                     /gene="rpmB"
                     /locus_tag="ACHKUN_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO6660007.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3454..4122)
                     /gene="radC"
                     /locus_tag="ACHKUN_001738"
     CDS             complement(3454..4122)
                     /gene="radC"
                     /locus_tag="ACHKUN_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO6660008.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4294..5514
                     /gene="coaBC"
                     /locus_tag="ACHKUN_001739"
     CDS             4294..5514
                     /gene="coaBC"
                     /locus_tag="ACHKUN_001739"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO6660009.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5492..5950
                     /gene="dut"
                     /locus_tag="ACHKUN_001740"
     CDS             5492..5950
                     /gene="dut"
                     /locus_tag="ACHKUN_001740"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO6660010.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6057..6653
                     /gene="slmA"
                     /locus_tag="ACHKUN_001741"
     CDS             6057..6653
                     /gene="slmA"
                     /locus_tag="ACHKUN_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO6660011.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6690..7331)
                     /gene="pyrE"
                     /locus_tag="ACHKUN_001742"
     CDS             complement(6690..7331)
                     /gene="pyrE"
                     /locus_tag="ACHKUN_001742"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO6660012.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7397..8113)
                     /gene="rph"
                     /locus_tag="ACHKUN_001743"
     CDS             complement(7397..8113)
                     /gene="rph"
                     /locus_tag="ACHKUN_001743"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO6660013.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8240..9103
                     /gene="yicC"
                     /locus_tag="ACHKUN_001744"
     CDS             8240..9103
                     /gene="yicC"
                     /locus_tag="ACHKUN_001744"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO6660014.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9324..10148
                     /gene="dinD"
                     /locus_tag="ACHKUN_001745"
     CDS             9324..10148
                     /gene="dinD"
                     /locus_tag="ACHKUN_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO6660015.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10440..11057
                     /gene="yicG"
                     /locus_tag="ACHKUN_001746"
     CDS             10440..11057
                     /gene="yicG"
                     /locus_tag="ACHKUN_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO6660016.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11054..12736)
                     /gene="ligB"
                     /locus_tag="ACHKUN_001747"
     CDS             complement(11054..12736)
                     /gene="ligB"
                     /locus_tag="ACHKUN_001747"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO6660017.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12994..13617
                     /gene="gmk"
                     /locus_tag="ACHKUN_001748"
     CDS             12994..13617
                     /gene="gmk"
                     /locus_tag="ACHKUN_001748"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO6660018.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13672..13947
                     /gene="rpoZ"
                     /locus_tag="ACHKUN_001749"
     CDS             13672..13947
                     /gene="rpoZ"
                     /locus_tag="ACHKUN_001749"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO6660019.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13966..16074
                     /gene="spoT"
                     /locus_tag="ACHKUN_001750"
     CDS             13966..16074
                     /gene="spoT"
                     /locus_tag="ACHKUN_001750"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO6660020.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16081..16770
                     /gene="trmH"
                     /locus_tag="ACHKUN_001751"
     CDS             16081..16770
                     /gene="trmH"
                     /locus_tag="ACHKUN_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO6660021.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16776..18857
                     /gene="recG"
                     /locus_tag="ACHKUN_001752"
     CDS             16776..18857
                     /gene="recG"
                     /locus_tag="ACHKUN_001752"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO6660022.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18842..19708)
                     /locus_tag="ACHKUN_001753"
     CDS             complement(18842..19708)
                     /locus_tag="ACHKUN_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660023.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(19711..20916)
                     /gene="gltS"
                     /locus_tag="ACHKUN_001754"
     CDS             complement(19711..20916)
                     /gene="gltS"
                     /locus_tag="ACHKUN_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO6660024.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            21196..22587
                     /gene="xanP"
                     /locus_tag="ACHKUN_001755"
     CDS             21196..22587
                     /gene="xanP"
                     /locus_tag="ACHKUN_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO6660025.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            22708..24417
                     /gene="yicH"
                     /locus_tag="ACHKUN_001756"
     CDS             22708..24417
                     /gene="yicH"
                     /locus_tag="ACHKUN_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6660026.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(24470..26788)
                     /gene="yicI"
                     /locus_tag="ACHKUN_001757"
     CDS             complement(24470..26788)
                     /gene="yicI"
                     /locus_tag="ACHKUN_001757"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO6660027.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(26798..28180)
                     /gene="yicJ"
                     /locus_tag="ACHKUN_001758"
     CDS             complement(26798..28180)
                     /gene="yicJ"
                     /locus_tag="ACHKUN_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO6660028.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            28473..28567
                     /locus_tag="ACHKUN_001759"
     tRNA            28473..28567
                     /locus_tag="ACHKUN_001759"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28507..28509,aa:Sec,seq:tca)
     gene            28646..28774
                     /gene="rhuM"
                     /locus_tag="ACHKUN_001760"
                     /pseudo
     CDS             28646..28774
                     /gene="rhuM"
                     /locus_tag="ACHKUN_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         7035 a         7838 c         7293 g         6745 t
ORIGIN      
        1 tggcgggtaa attgctattt aagtcaaaaa cgggaaaagt aatggtaaag ccacagctaa
       61 atacatagaa tccccagcac atccataagt cagctattta ctatgctcga attgctttac
      121 accgcccttc tctaccttat tcagccgctg atctggatac ggctctgggt gcgcggacgt
      181 aaggctccgg cctatcgaaa acgctggggt gaacgttacg gtttttaccg ccatccgcta
      241 aaaccaggcg gcattatgct gcactccgtc tccgtcggtg aaactctggc ggcgatcccg
      301 ttggtgcgcg cgctgcgcca tcgttatcct gatttaccga ttaccgttac aaccatgacg
      361 ccaaccggtt cggagcgcgt acaatcggct ttcgggaagg atgttcagca cgtttatctg
      421 ccgtatgacc tgcccgatgc gctcaaccgt ttcctgaata aagtcgaccc caaactggtg
      481 ttgattatgg aaaccgaact atggcctaac ctgattgcgg cgctacacaa acgtaaaatt
      541 ccgctggtga tcgctaacgc gcgactctct gcccgctcgg ccgcaggtta tgccaaactg
      601 ggtaaattcg tccgtcgctt gctgcgtcgt attacgctga ttgctgcgca aaatgaagaa
      661 gatggtgcac gttttgtggc gctgggcgca aaaaataatc aggtgaccgt taccggtagc
      721 ctgaaattcg atatttctgt aacgccgcag ttggctgcta aagccgtgac gctgcgccgc
      781 cagtgggcac cacaccgccc ggtatggatt gccaccagca ctcacgaagg cgaagagagt
      841 gtggtgatcg ccgcacatca ggcattgtta cagcaattcc cgaatttatt gctcatcctg
      901 gtaccccgtc atccagaacg cttcccggat gcgattaatc ttgtccgcca ggctggacta
      961 agctatatca cacgctcttc aggggaagtc ccctccacca gcacgcaggt tgtggttggc
     1021 gatacgatgg gcgagttgat gttactgtac ggcattgccg atctcgcctt tgttggcggt
     1081 tcactggttg aacgtggtgg gcataatccg ctggaagctg ccgcacacgc tattccggta
     1141 ttgatggggc cgcatacttt taactttaaa gacatttgcg cgcggctgga gcaggcaagc
     1201 gggctgatta ccgttaccga tgccactacg cttgcaaaag aggtgtcctc tttactcacc
     1261 gacgccgatt accgtagttt ctatggccgt catgccgttg aagtactgta tcaaaaccag
     1321 ggcgcgctac agcgtctgct tcaactgctg gaaccttacc tgccaccgaa aacgcattga
     1381 ggttgttatg caaaaacggg cgatttatcc gggtactttc gatcccatta ccaatggtca
     1441 tatcgatatc gtgacgcgcg ccacgcaaat gttcgatcac gttattctgg cgattgccgc
     1501 cagccccagt aaaaaaccga tgtttaccct ggaagagcgt gtggcgctgg cacagcaggc
     1561 aaccgcgcat ctggggaacg tggaagtggt cgggtttagt gatttgatgg cgaacttcgc
     1621 ccgtaatcaa cacgctacgg tgctgattcg tggcctgcgt gcggtggcgg attttgaata
     1681 tgaaatgcag ctggcgcata tgaatcgcca cttaatgccg gaactggaaa gtgtgtttct
     1741 gatgccgtcg aaagagtggt cgtttatctc ttcatcgttg gtgaaagagg tggcgcgcca
     1801 tcagggcgat gtcacccatt tcctgccgga gaatgtccat caggcgctga tggcgaagtt
     1861 agcgtagcgt ttatgccgga tggtatgcca tccggcgcgc atgaattact tctggcactg
     1921 ccgacaataa aacgttgccc gctgcgcatg tttagtcgcc acaatcggcg taccgcacac
     1981 ccgacacggc tcgccttttc gcccgtaaac ctgcaattcc tgtgcgaaat agcccggttt
     2041 accatcactt tgcagaaaat ctttcagcgt tgtaccgccc tgctcaatcg aacgcagcaa
     2101 caccgcttta atcacccgag ctaacaattc acactctgcc agcgacagtg atgacgccag
     2161 ccgatccgga tggatccccg ccgcaaacag tgattcgctg gcatagatat tccctacccc
     2221 taccaccagc ttgttatcca tcagccacgg tttaatcgcc gtttttttct tcgcgcactt
     2281 ctgatgcaga tactcaccat tgaaatcgtc gctgagcggc tccggtccaa gatgggccag
     2341 cacattatgc ccttccagct ctttggtcca cagccaggca ccaaagcggc gcggatcggt
     2401 gtagcgcagc actttaccgt tgctcatcac caaatccaca tggtcatgct tttcaggggg
     2461 aagttcttct ggaaggatgc gcaggctgcc agacatcccc aaatgaatga taatccagcc
     2521 ctcaggcagc tccagcagca aatatttagc ccgccgctgc acgctaagca ctggttggtc
     2581 gcttaaacgg tagatctctt ctgaaaccgg ccagcgcaag cgtccgttgc gcaccactgc
     2641 atgaagaatg gttgcaccaa cgagatgcgg ttctatgccg cgacggctgg tttcaacttc
     2701 gggtaattca ggcatagcat ctccaggaat gaacagatgc aatcaatatg ggggcaagca
     2761 gataacaaaa aaccccgccg gagcgaggtt ttttgttaca tcaaagcgag aattatttga
     2821 ttttcgcttc tttgtagatc acgtgctgac gaacaactgg atcgaatttt ttcagttcca
     2881 gtttttccgg cttagtacgt ttgttcttcg tagtggtata gaagtgacca gtaccagcag
     2941 aagaaaccag cttgattttc tcacgaatac ctttagccat gatttatttc ctctaagtac
     3001 ttagtacttt tcgccacggg cacgcagttc agccagaact gtatcgatgc cttttttatc
     3061 gattacacgc atacctttag cagatacgcg cagggtgaca aaacgcttct cgctctcaac
     3121 ccagaaacgg tgagagtgca ggttcggcag gaaacggcgt ttagtcgcgt tcagtgcgtg
     3181 ggaacggttg ttaccggtca ccggacgctt gccagtaact tggcagactc gggacatgtc
     3241 tattctccaa aaatcaaatt agctcgagct tcgtatgggg tattggcgcc tcgtcaggct
     3301 ttacagcccg gtcatcgcag ttctatgtga actctcgatt gccaggccca aatgccaaac
     3361 ccgagattct caaaggtggc gtagtatacg ctgactcagc gatgtgctca agtcccgaac
     3421 agacaaagat cccgaaggat cgcgcatagc gggttaaatc cagccgcgtt cggcaaaaga
     3481 aacatactct ccacgcccaa tcacgatatg gtcgagcacg cgtaaatcca tgaactgaca
     3541 actctttatt atccgttcag taatgagttt atccgcttta ctgggttcag cacaacccga
     3601 agggtgatta tgtgcaagga tcagcgccga ggcgtttatt tttatcgctt cgcggataat
     3661 ttcccgagga tggacttcaa catggtttag cgtgccggaa aaaagacggc tatgcgttat
     3721 aacccggtgt tgtgagtcga gaaagatcac cataaagatc tcccgctcct cacccgtgag
     3781 ctggctttgt aaaaattccc gcgtcatctc cgggctgagt aaagggcttt cttcacgcat
     3841 ccgcacgttg tagtaacgcc gcgccagttc agcaatcccc tttaactggg caaatttcgc
     3901 tacgccaatt ccatgaacgc cactaaattg ttcatattca gaggttaaca agccataaag
     3961 agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac cgcgcgttcc
     4021 ggtacgcaga aataacgcca gcagctcgac atccgttaag gcgctaatac caaacttcag
     4081 cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct cctttgtggt
     4141 gctcgcatcc tgtcatagcc gttccgttaa atcgacggcc agttttcaat cctggaaagc
     4201 gcctcgcaaa gtgattctca ggtgaaggca cgacaacaaa agtattgtga taaaatcgcc
     4261 aacttctggt gtcacccaac aggaaaaatc atcatgagcc tggccggtaa aaaaatcgtt
     4321 ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg tcgtttgcgc
     4381 gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt tatcacccca
     4441 cttagcttgc aggcggtttc tggttatccc gtttccgaca gtttgctgga cccggcagcc
     4501 gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat tctcgcccct
     4561 gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct ggtatcgacg
     4621 atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca gcagatgtac
     4681 cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt gctcatctgg
     4741 gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat gctcgatccg
     4801 ttaaccattg tggatatggc ggtggcgcat ttttcgcccg tcaacgacct gaaacatctg
     4861 aacattatga ttaccgccgg tccgacgcgt gaaccgctcg atccggtgcg ttatatctct
     4921 aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg tcgtggcgcg
     4981 aacgtcacgc tggtatcagg tccggtttca ctaccgacgc caccgtttgt taaacgtgtt
     5041 gatgtgatga ctgcgctgga aatggaagcc gccgtgaatg cttctgtaca gcagcaaaat
     5101 atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc cccagagaaa
     5161 atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa aaaccccgat
     5221 atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg atttgccgcc
     5281 gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa ccttgatctg
     5341 atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa caacgcatta
     5401 caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga gctccttggc
     5461 caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg ttaagattct
     5521 ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg gctctgccgg
     5581 acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg acactacgct
     5641 ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa tgatgctgcc
     5701 gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag gattgatcga
     5761 ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg acagcttcac
     5821 cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac aggctgaatt
     5881 taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg gtcactctgg
     5941 tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac atttcgtttg
     6001 cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt tgtaacatgg
     6061 cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct ctggcgctga
     6121 tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc gcctctgtcg
     6181 gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg ttcgatagcc
     6241 tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg aaagatgaga
     6301 aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt ggtgagcgta
     6361 atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag gatcgcctgc
     6421 aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag gtattgcgtg
     6481 aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg gcaagccaga
     6541 tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt aaataccgcc
     6601 cgacggatga ttttgacgcc cgctggccgc taattgcggc acagttgcag taatatgacg
     6661 ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg ataggcctta
     6721 accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat caggtctttc
     6781 agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg aatcgccgaa
     6841 atctcgccgc gcccccgttc ctgacgatca agcgaaatca acacgccagc aagcgtcgcg
     6901 ccattagcct gaataatctc catcgattcg cgaatcgccg ttccggcggt gatcacatca
     6961 tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc ttcaccgtgg
     7021 tcttttgcct ctttgcggtt aaagcagtac ggcaggtcaa ggtcatgatg ctccgccagc
     7081 gccacggcgg tagtggtggc aatcgggatc cctttgtaag cagggccaaa cagcagatcg
     7141 aactcaaggc cggaatccac caacgcttca gcgtaaaaac ggcctaacag tgccagatcg
     7201 cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttgcgccc ggacttcaac
     7261 gtaaactcgc caaactttaa cacctgcttg ccaagcgcaa attcaataaa ctggcgctga
     7321 tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa aggcgactca
     7381 tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca atggattcga
     7441 ttccccctcg ggccagcgcc aacaaggtga gtagctcttc atgggtgaac ggctcgcctt
     7501 ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg ttcatgtcgg
     7561 tctctgcggc tgagtcttca acgtattcca gatcgcaaat tgcttcaccg tttacaatac
     7621 cgacagaaac tgcggctacc atccctttca tcgggttggt tttcagcttg ccgttttcca
     7681 ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgac gcggtacgcg
     7741 tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca cccagcgctt
     7801 tcaaatctac tgccgcgcga agagcacggg cgatcagacg ctggatttcc attgtgcgtc
     7861 caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa cgtggcagca
     7921 tgccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc ggcacgcctt
     7981 cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc accgagcctt
     8041 ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta ttgctacggc
     8101 ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca ttatacggac
     8161 ttccggcggt tattcctatc ctgacaaggc atcgatggct ataatccttc cacctctcct
     8221 tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc gtgaaatcaa
     8281 gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt atctggaaac
     8341 ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg agcgtattcg
     8401 ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag atgttagcgc
     8461 gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg ccgcgaactg
     8521 ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc gctggccggg
     8581 cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc tcgcggcgtt
     8641 ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg cgctgaaagc
     8701 attgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc gcgcccatat
     8761 gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag atgctcaagt
     8821 gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac gaattgacgt
     8881 tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca ttctgaagaa
     8941 aaaagaagcc gttggtcgtc gtctggactt tatgatgcag gaattcaacc gcgagtcgaa
     9001 cactcttgcg tcgaagtcta tcaatgccga agtgacaaac tccgcgattg agctgaaagt
     9061 gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa cactaattct
     9121 tacttttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt cttccccgca
     9181 acttcaattc tgttttgcgt gcctgctcca gattttgcga tgtttttttg cccagcacac
     9241 tgagaacgtg agatactcac aactgtatat aaatacagtt acagatttac tttctttgca
     9301 atcgatatca catggagtgg gcaatgaacg aacatcatca accttttgaa gaaatacggc
     9361 attatggcac tgaagggcaa gaattttggt cagctagaga attggcaccg ttactggatt
     9421 atcgggactg gcgcaatttt caaaaggtac tggcccgcgc gactcaagcg tgcgaagcca
     9481 gcaatcaagc tgcatctgac catttcgttg aaaccaccaa aatggttgta ttaggttcag
     9541 gggctcaacg agaacttgaa gatgttcatc tctcccgcta tgcctgttac ctggtagtac
     9601 aaaacggcga ccctgcgaaa ccggttattg cggcagggca aacttatttt gctatccaga
     9661 cccgacggca ggagctggct gacgatgaag ctttcaggca acttcgtgaa gacgaaaagc
     9721 gtctttttct gagaaatgaa ttgaaagaac ataacaaaca attggttgag gcagcgcagc
     9781 aagctggggt agcgacagct atcgatttcg ccatatttca gaatcatggt taccgggggc
     9841 tgtatggtgg attagatcag aaagccatcc atcagcggaa ggggctgaaa aagagtcaga
     9901 agatcctgga tcatatgggt tcaacagaac tggcggctaa tctctttcga gctacccaaa
     9961 cagaagaaaa actcaagcgg gatggcgtga attcaaaaca gcaagctaat accacacact
    10021 ttgacgtggg tagcaaggtg aggcaaacca ttcaggaact tggcggaacc atgcctgagg
    10081 agttgccgac cccgcaggtc agcatcaagc agttggaaaa cagtgtaaaa attacagaga
    10141 agaaatagca gaaactaaaa actctttttg ttgattgaga tacccgatgc gtaagccaaa
    10201 gtccaggtgc ggtgaacaca atggctaaac attgcacctt tcttttccac ccctcaattt
    10261 taacctattt tttcttatgc attttctcag acaagaagtc agaagaatgc atctacgcta
    10321 cagaaaatag cgatttcaca taactacaag ttatcaattt cctctcacct taaaaaatct
    10381 caatcgtgac aatacgcaca aatcgctacc ctgccagaca gatttttagg gagagaacca
    10441 tgctgttaca cattttgtat ttggttggca ttactgccga agccatgacg ggggctctgg
    10501 cggccgggcg acgccgcatg gatacatttg gcgtaattat tattgcgacc gccaccgcaa
    10561 ttggcggagg gtcagtgcgc gatattctgc tgggccacta tccgctcggc tgggtcaaac
    10621 acccggaata tgtgattatc gtcgctaccg ccgcagtact caccactatc gttgctcctg
    10681 taatgcctta cctgcgcaaa gtgtttctgg tgctcgatgc gcttggactg gtggtctttt
    10741 ctatcatcgg cgcacaggtt gcactggata tggggcacgg cccaattatt gccgttgtcg
    10801 cggcggtaac caccggcgtg tttggcggcg ttttgcgcga tatgttctgt aaacgtatcc
    10861 cactggtatt ccagaaagag ttgtatgccg gtgtctcatt tgcctccgcc gtgctgtaca
    10921 tcgcgctgca acactatgtt tctaaccatg atgtggtaat tatctccacc ctggtattcg
    10981 gcttttttgc ccgcttactg gcgctacgcc ttaagctggg attaccggtc ttttactaca
    11041 gccacgaagg acactaaggt tcaaaacctg tgatctgctg ggcagccagc caactgccca
    11101 gcttcttgat ttgcgcattt tccttccatt caataacctg tctggcgcgc cccgatccag
    11161 tccccggcag ctgctgccag aactgctccg tgctaaataa aagttgcgac caggaccgtt
    11221 catcactggc attaagcgcc gcccgggtta gcggtattcc cattgccatc acccagcgag
    11281 taaaaggctg cttacgagcc agattaaacc gatgccatag ctgcgcactt ttacttttcg
    11341 cgatccccgg cgtgttctgt aattgctctg gcgttaataa aagccaggaa aagatatgct
    11401 caaagcgatg agtctgatgc agcgcgcgcc agccggcctc accaatacca tccagcccca
    11461 gaacctgttt tgaccccagc cagactaagc gtgaaatgaa ctgttcctga caaacatcag
    11521 aagcaaagta gcaggtcaac gagttaaagc ggttttctgg tggtgtcggt tttgtacgtt
    11581 ctgcaccgcg ccagaccaca tcatcaatgc gaggaatacc ctgaccggca aggctgacga
    11641 ggatctgatc accaggcgca atatcccact cctgccagcg cctgacggaa ccaatattca
    11701 cccgctggac ttttttatca tccagcatga caggtgcgag tgacgcaacc accgatattt
    11761 taccgctctt acccaccgca aactgaatcg ccttcacttc ggcaacctga gctacaggtt
    11821 gatatttcca ggccaccagc cattctgcct ggcctggtag ccaatggcgg gattcgggtt
    11881 ctttcgcccc tcgcacaacc acgccatcgg tgacgaaggg taatttcgct ttccaccact
    11941 cattgcgtac gcgcgcaacc tcatcagcat ttttaaccgc acgggtatac ctctgcgtta
    12001 gagtaaaacc tgcggtagcc agctccttta aacgatcggt cattaactgc ggtccatccg
    12061 gccatgccca gacaaaaacg cccagagaat tcagcgtgtc gctatcgtcc tggcgcatca
    12121 tcaggccagc aacttttgcg cgggcattta ttccccccat ttgttgttgg atatgcccct
    12181 cgcgctggag aaatatttcc ccctgaagcg tactgttggc taaaggcccg ctaacggttt
    12241 gcggcacagc ggaaattaag ctaactttct gcgtccagtc ctcgcctttc aggccattac
    12301 cgcgactgat tgctttgttc agtttcccgt cccgataaac cagggttacc gcaacgccat
    12361 caacttttgg ctgcacccaa agatcgctac gttctcgcat ccacagactt aatgcgtttt
    12421 tatccgccat tttgcgtaca cccgtatggg caaccggatg cataaccgca ccatttaaag
    12481 gcggcatcat cacgtcgcgg ggctcgctca caaagcagcg ttgccactgc gtaagacggg
    12541 cgcttaactg atcgtaaaca ccgtcttcca cctcactttt tccttccttc cagtagtcat
    12601 cgtcccactg ttttatttgc tgttgcaggc gggaaatttc ttcctgtgct ctggctggcg
    12661 accaggccgg acagaccgcc cacacagatg attgccagca caagatactt attaatatcg
    12721 ccatccatac tttcatcttc acctccgctg tagtcaggct ggcagatata caacgtggtg
    12781 aaggcaaagc cgagtggcaa aaacggagtc tgcgaggacg cttcctgaga atcgtcttta
    12841 ttgcagtaaa tcacaggcaa atgcggaaac agctacgcaa aacgcaacaa ctttgcgcaa
    12901 aaagtgtgag caagggctac gtcacatggc cgcgccgtgt ataataagct cgtatgtagg
    12961 ctttatttcg ctaatcacat acgaaagata ctcatggctc aaggcacgct ttatattgtt
    13021 tctgccccca gtggcgcggg taaatccagc ctgattcagg ctttattaaa aacccaaccg
    13081 ttgtatgaca cccaggtttc tgtttcacac accacacgcc aaccgcgtcc tggtgaagtc
    13141 cacggtgaac attatttctt tgttaatcat gatgaattta aagaaatgat tagcagagat
    13201 gcgttcctcg aacacgcaga agtttttggt aattactatg gcacttcgcg tgaggccatt
    13261 gagcaagtac tggcgaccgg tgtcgatgtt tttctcgata tcgactggca gggcgcgcag
    13321 caaattcgcc agaagatgcc gcacgcgcgg agtatcttta ttttaccgcc gtccaaaatt
    13381 gaactggacc gccgtctacg cggtcgcggt caggacagcg aagaggtcat tgcaaagcgt
    13441 atggcgcaag ctgttgcaga aatgagccat tacgccgaat atgattatct gattgtgaat
    13501 gatgacttcg ataccgcgtt gaccgatttg aagaccatta ttcgcgccga acgtctgcgc
    13561 atgagccgcc aaaagcagcg tcatgacgct ttaatcagca aattgttggc agactgaacc
    13621 tgatttcagt atcatgccca gtcatttctt cacctgtgga gctttttaag tatggcacgc
    13681 gtaactgttc aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt actggtcgcc
    13741 gcgcgtcgcg ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc ggaagaaaac
    13801 gataaaacca ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa caaccagatc
    13861 ctcgacgttc gcgaacgcca ggaacagcaa gagcaggaag ccgctgaatt acaagccgtt
    13921 accgctattg ctgaaggtcg tcgttaatca caaagcgggt cgcccttgta tctgtttgaa
    13981 agcctgaatc aactgattca aacctacctg ccggaagacc aaatcaagcg tctgcggcag
    14041 gcgtatctcg ttgcacgtga tgctcacgag gggcaaacac gttcaagcgg tgaaccctat
    14101 atcacgcacc cggtagcggt tgcctgcatt ctggccgaga tgaaactcga ctatgaaacg
    14161 ctgatggcgg cgctgctgca tgacgtgatt gaagatactc ccgccaccta ccaggatatg
    14221 gaacagcttt ttggtaaaag cgtcgccgag ctggtagagg gggtgtcgaa acttgataaa
    14281 ctcaagttcc gtgataagaa agaggcgcag gccgaaaact ttcgcaagat gattatggca
    14341 atggtgcagg atatccgcgt catcctcatc aaacttgccg accgtaccca caacatgcgc
    14401 acgctgggct cacttcgccc ggacaaacgt cgccgcatcg cccgtgaaac tctcgaaatt
    14461 tacagcccgc tggcgcaccg tttaggtatc caccacatta aaaccgaact cgaagagctg
    14521 ggttttgagg cgctgtatcc caatcgttac cgcgtaatta aagaagtggt gaaagccgcg
    14581 cgcggcaacc gtaaagagat gatccaaaaa atcctctctg aaatcgaagg gcgtttgcag
    14641 gaagcgggaa ttccgtgccg cgtcagtggt cgcgagaagc atctttattc gatttactgc
    14701 aaaatggtgc tcaaagagca gcgttttcac tcaatcatgg acatctacgc tttccgcgtg
    14761 atcgtcaatg attccgacac ctgttatcgc gtgctcgggc aaatgcatag cctgtacaag
    14821 ccgcgtccgg gtcgcgtgaa agactacatc gccattccaa aagcgaacgg ctatcagtct
    14881 ttgcacacct cgatgatcgg cccgcacggt gtgccggttg aggtccagat ccgtaccgaa
    14941 gatatggatc agatggcgga gatgggtgtt gccgcgcact gggcttataa agagcacggc
    15001 gaaaccagca ccaccgcgca aatccgcgcc cagcgctgga tgcaaagcct gctggagctg
    15061 caacagagcg ccggtagttc gtttgaattt atcgagagcg ttaaatccga tctcttcccg
    15121 gatgagattt acgttttcac accggaaggg cgcattgtcg agctgcctgc cggtgcaacg
    15181 cccgtcgact tcgcttatgc agtgcatacc gatatcggcc atgcctgcgt gggcgcacgc
    15241 gtcgaccgcc agccttaccc gctgtcgcag ccgcttacta gcggtcagac cgttgaaatc
    15301 attaccgctc cgggcgctcg cccgaatgcc gcttggctga actttgtcgt tagctcgaaa
    15361 gcgcgcgcca aaattcgtca gttgctgaaa aacctcaagc gtgatgattc tgtaagcctg
    15421 ggccgtcgtc tgctcaacca tgctttgggt ggtagccgta agctcaatga aatcccgcag
    15481 gaaaatattc agcgcgagct ggatcgcatg aagctggcaa cgcttgacga tctgctggca
    15541 gaaatcggcc ttggtaacgc aatgagcgtg gtggtcgcga aaaatctaca acatggggac
    15601 gcctccattc caccggcgac tcaaagccac gggcatctgc cgatcaaagg cgctgatggc
    15661 gtgctgatca cctttgcgaa atgctgccgc cccattcctg gcgacccgat tatcgcccac
    15721 gtcagccccg gtaaaggtct ggtgatccac catgaatcct gccgtaacat ccgtggctac
    15781 cagaaagagc cagagaagtt tatggctgtg gaatgggata aagagacggc gcaggagttc
    15841 atcaccgaaa tcaaggtgga gatgttcaat catcagggtg cgctggcaaa cctgacggcg
    15901 gcaattaaca ccacgacctc gaatattcaa agtttgaata cggaagagaa agatggtcgc
    15961 gtctacagcg cctttattcg tctgaccgct cgtgaccgtg tgcatctggc gaatatcatg
    16021 cgcaaaatcc gcgtgatgcc agacgtgatt aaagtcaccc gaaaccgaaa ttaatgtttt
    16081 atgaacccaa cacgttatgc acgcatctgc gaaatgctcg ccaggcggca gcctgatctg
    16141 accgtctgca tggagcaggt ccacaaacct cataacgttt ctgcgattat tcgtaccgca
    16201 gatgccgttg gcgtacatga agttcacgcc gtctggcctg gtagccgcat gcgcaccatg
    16261 gcttcggcag cggcgggtag taacagctgg gtacaggtga aaacacaccg caccattggc
    16321 gatgccgtcg ctcatctcaa aggccagggc atgcagattc tggcaaccca tctttctgat
    16381 aacgctgtcg atttccgcga aattgattac actcgcccga cctgcatttt gatgggacag
    16441 gagaaaacgg gcatcacgca ggaagcattg gccctggcgg atcaggacat catcattccg
    16501 atgatcggca tggtgcagtc gctgaatgtg tccgttgcct cagccctcat tctttacgaa
    16561 gcccagcgtc agcggcaaaa tgcaggcatg tacctgcgtg aaaacagcat gttgccggaa
    16621 gcagagcaac aacgcctgtt gtttgaaggc ggctatccgg tgctggcgaa agtcgcaaaa
    16681 cgcaaaggcc tgccttatcc ccacgtcaat cagcaaggcg agatcgaagc tgatgccgac
    16741 tggtgggcta ctatgcaggc tgcagggtaa gtgccatgaa aggtcgcctg ttagatgctg
    16801 tcccactcag ttccctaacg ggcgttggcg cagcacttag taacaaactg gcgaaaatca
    16861 acctgcatac cgtgcaggat ctactcttac accttcccct gcgctacgaa gatcgcaccc
    16921 atctctaccc catcggagaa ctactgccgg gcgtttatgc cacggtggaa ggcgaagtgc
    16981 tgaactgcaa tatctccttc ggcggtcggc ggatgatgac ctgccagatc agcgacggtt
    17041 ccggcatcct caccatgcgc tttttcaatt tcagcgcggc aatgaaaaat agcctggcgg
    17101 cgggccgccg tgtactggct tatggcgaag caaagcgcgg taaatatggt gcggagatga
    17161 tccacccgga ataccgcgtg cagggcgatc tcagcacgcc agaattacag gaaacgctca
    17221 cgccggttta tccaacaacg gaaggcgtaa agcaggccac gctgcgtaaa ttaaccgacc
    17281 aggcgctgga tctgctcgac acctgcgcca ttgaagaact cctgccgccg gaactgtcac
    17341 aaggaatgat gacgctaccg gaagcgttgc gcactttgca ccgcccgcca ccgacgctac
    17401 agcttagcga tctggaaacc gggcagcatc cggcgcaacg tcgtctgatt ctggaagaac
    17461 tgctggcgca caacctcagc atgttagcct tacgtgccgg agcacagcgt tttcatgccc
    17521 agccgctgag cgccaatgac acgctgaaaa ataaactcct cgccgcctta ccgttcaagc
    17581 caacgggcgc acaggcacgc gtagtggcgg agatcgagcg cgatatggcg ctggatgtgc
    17641 cgatgatgcg tctggtgcag ggcgatgtag gttccggtaa aacgctggtc gccgccctcg
    17701 ccgcgttgcg tgcgattgcc cacggcaaac aggtagcatt gatggcacca accgaattac
    17761 tcgccgagca gcacgccaat aacttccgca actggtttgc accgctcggt atcgaagtgg
    17821 gctggctcgc cggtaagcag aaaggtaaag cacggctggc acagcaggaa gccatcgcca
    17881 gcggtcaggt gcagatgatt gtcggtacac acgccatctt ccaggaacag gtgcagttta
    17941 acggcctggc gctggtgatt atcgacgaac agcatcgttt tggcgtgcat cagcgtctgg
    18001 cattgtggga gaaaggccag cagcagggct tccatccgca tcagttgatc atgaccgcca
    18061 cgccgatccc ccgcacgctg gcaatgactg cgtatgccga tctcgatacc tcggtgatag
    18121 atgagctgcc gccaggccgc acgccagtga ctacggtcgc tattcctgat acccgccgta
    18181 ccgacatcat tgaccgcgtg caccacgcct gcataactga aggtcgtcag gcatactggg
    18241 tttgtacgtt gattgaagag tcggaattgc tggaagcgca ggcggcggaa gctacctggg
    18301 aagagttgaa actggcgcta ccagagttga acgttggcct ggtacacggg cggatgaaac
    18361 ctgccgagaa acaggcggtg atggcgtcgt ttaaacaagg tgagctacac ctgctggttg
    18421 ccacaaccgt tattgaagtc ggcgttgatg tgcctaacgc cagtctgatg attatcgaaa
    18481 acccggagcg tctgggtctg gcgcagttac accagcttcg cgggcgtgta ggtcgtggtg
    18541 cggtggcttc tcactgcgtg ctgctctaca aaacgccgct ctccaaaacc gcgcaaattc
    18601 ggctgcaagt gctgcgcgac agcaacgacg gttttgtgat tgcacagaaa gacttagaga
    18661 ttcgcggacc gggtgaactg ttaggtacgc gtcagacggg taatgctgag tttaaagtgg
    18721 cggatttact gcgcgatcag gcgatgatcc cggaagttca gcgcctggcg cgacatattc
    18781 acgaacgtta cccacaacaa gcaaaagccc tgatagaacg ctggatgccg gagacggaac
    18841 gttactccaa cgcatgacca gactcatcac aagacaagtc acgcagtaaa tcttcaatct
    18901 ggatagtgag gatatccatt gcctcatacg catcctgtgc aattcgcgcc gccattgcgc
    18961 cttccggttc accacgcaga atttcagccg ctgcctgtac gccagccaca atcatttcag
    19021 gtgttaacat ttggcgaaga aaagcgcgat tatcagagga ccagccatct ttatggccta
    19081 atggatgccc ccagcctatc cattcttgcg accataacgg attggcttct atgggataaa
    19141 ttagatgcgc ggcatactga atagcacaca ccgcttttcc tggcgtatcc agcgcccacc
    19201 agcgcgacca gagagaacga attaacggcg cggccccacc cagagtattg aacaccgcca
    19261 gccagcaaag aacgttattg aaaccacgct cgttatccat acgctgcaac gcggtgtcca
    19321 gtaaaaattg ccgtacctgc tggcgttggg aagcattcat catctcctgc cagagatagg
    19381 gccgacacaa tgccttcatt aaggattctt cgaagtgatc accataaccg ccagctaaaa
    19441 cggtttcatg ccattgcgcc aggcagagcg gaaacagata gcgaaacaac tctatttgca
    19501 tcggttcgtt ataaacgaga ttgagtacat agtaagcact caaatcccat tcgctgatct
    19561 gctgccactc tttagccgcc agcgccttga gttcggcatc tgcgtaatca aattccgttt
    19621 cccatacaca gagcactggc ggctcatcac caaaaatatg gcgacagtgt tcacgtagcg
    19681 tttccggttc cttccgacgc tttttcattt ttaaccggca aaaatcggca acattaaata
    19741 cagcttaatc accagcgcat tgacgatatc gataaagaac gcaccgacca tcggcaccac
    19801 caaaaacgcc atgtgcgacg ggccaaagcg ttcagtgatc gcctgcatgt tggcgattgc
    19861 cgttggcgtt gcaccgaggc caaaaccaca gtgacccgca gccagcactg ccgcatcgta
    19921 gtttttgccc atcatgcgcc aggtaacgaa gatggcatac aacgccatga agatggtctg
    19981 taccaccaga atcgccagca tcggcagcgc cagcgaagcc agctcccaca gtttcagccc
    20041 catcagcgcc atcgccagga acaacgacag gcttacgtta cccagcacgg ataccgcacg
    20101 ctcaaagacg cggtaaaagc ccatcattga cagaccgttg ctcagaatca cgccaacaaa
    20161 cagtacacag acgaaggtcg gcagttcaaa agcagtgcca gccaaaagtt gcgcaacaat
    20221 tttccccacc gtcaggcaga tagcaatcag cgcgatagtt tcaatcagca ccagcgaggt
    20281 gatcatgcgt cccacatccg gcttttcaaa cgccgtcggg acttcctggt catccggaat
    20341 accgttcggc gtggtggagt gtttcaccag atagcgcgcc accggaccgc caatcaagcc
    20401 gcccagcacc agaccgaacg ttgcacaggc catcgccact tccgtcgcat tggtgaagcc
    20461 ataacgttca atgaacaatt tactccacgc agcgcccgta ccgtgaccgc cggaaagcgt
    20521 aatagaaccg gccaacagcc ccatcagcgg atcaagccct aacaggctag ccataccaat
    20581 gccaatggca ttttgcatca ccaacagacc aacaaccaca atcaagaaga tgccaaccac
    20641 acgcccaccg gcacgcaaac tggcaatgtt ggcgttcagg ccaatggtgg cgaagaaagc
    20701 cagcattaac ggatcgcgca gggacatatc aaagttgact tcccagccca tgcttttttt
    20761 cagtactagt agcgccagcg ccaccaacaa accacccgca acaggttccg gtatggtgta
    20821 tttcttcaaa aaggagacgg aatggaccaa cttacgcccg agcagcaacg tcagcgttgc
    20881 ggcaacaagc gttgctaaag tatcgagatg aaacatagtt actcctttgt atccgcatca
    20941 ctctttcata ctcgtcatac ttcatcgcgc agtggcaact tcatgaacta tgacgggtat
    21001 cgagttatgc tttcctgggt caaattctca gctgttaatg aacaaatggc atggatttta
    21061 agcagaaaaa taccaaaagt tatataaaaa agtgcattta tctcatttaa atatttagat
    21121 gaggatgtgg caaacgtttg cttttctctt tcggtcagct aaaatgcccg ctttgctacc
    21181 acgggattgt tttcgatgtc tgtttccacc ctcgagtcag aaaatgcgca accggttgcg
    21241 cagactcaaa acagcgaact gatttaccgt cttgaagatc gtccgccgct tcctcaaacc
    21301 ctgtttgccg cctgtcagca tctgctggcg atgttcgttg cggtgatcac gccagcgcta
    21361 ttaatctgcc aggcgctggg tttaccggca caagacacgc aacacattat tagtatgtcg
    21421 ctgtttgcct ccggtgtggc atcgattatt caaattaagg cctggggtcc ggttggctcc
    21481 gggctgttgt ctattcaggg caccagcttc aactttgttg ccccgctgat tatgggcggt
    21541 accgcgctga aaaccggtgg tgctgatgtt cctaccatga tggcggcttt gttcggcacg
    21601 ttgatgctgg caagttgcac cgagatggtg atctcccgcg ttctgcatct ggcgcgccgc
    21661 attattacgc cgctggtttc tggcgttgtg gtgatgatta tcggcctgtc gctaattcag
    21721 gttgggttaa cgtccattgg cggcggttac gcagccatga gcgataacac cttcggcgca
    21781 ccgaaaaatc tgctgctggc aggcgtggtc ttagccttaa ttatcctgct taaccgtcaa
    21841 cgtaaccctt acttacgcgt ggcctcactg gtgattgcga tggcggccgg atatgcgctg
    21901 gcgtggttta tgggcatgtt gccagaaagc aacgaaccga tgacgcaaga actgattatg
    21961 gtgccaacgc cgctctatta cggtcttggc attgaatgga gtctgctgct gccgctgatg
    22021 ctggtcttta tgatcacttc gctggaaacc attggcgata tcacggcgac ctctgacgtt
    22081 tccgaacagc cagtgtccgg tccgctgtac atgaaacgcc tgaaaggcgg cgtgctggca
    22141 aacggcctga actcgtttgt ttcggcggtg tttaacacct tcccgaactc ctgcttcggg
    22201 caaaacaacg gagtgatcca gttgactggt gttgccagcc gctatgtcgg ttttgtcgtc
    22261 gcgctgatgt tgatcgtgct gggtctgttc ccggcagtga gcggttttgt acaacacatt
    22321 ccagaaccgg ttctgggcgg cgcaacgctt gtaatgtttg gcactatcgc cgcctccggt
    22381 gtgcgtatcg tttctcgtga accgctgaac cgtcgggcga ttctgattat cgcgctgtcg
    22441 ctggcggttg gtctgggcgt gtctcagcag ccgctgattt tgcagtttgc ccctgaatgg
    22501 ctgaaaaacc tgctctcctc cgggatcgcc gcgggcggta ttactgccat cgtgctgaat
    22561 ctgattttcc caccagaaaa acagtaattc actggcggcg gtaagataat taccgccgca
    22621 gtctcacctc tttcaccatt cccgccttga ggattgcgcg taaatcgtgc ataactccct
    22681 tatgtgcatt tcacgggatg gaagaccatg aaatttattg ggaagctgct tctctacatt
    22741 ctcatcgctc tgttagtggt gatcgctggc ctctattttc ttctgcaaac ccgctgggga
    22801 gcagaacata tcagcgcatg ggtttccgag aatagcgact atcatctggc cttcggggcg
    22861 atggatcacc gtttttccgc gccatctcat atcgtgctgg agaacgtcac gtttggccgt
    22921 gatggccagc ccgcgaccct ggtggcaaaa agtgtcgaca ttgcgctaag cagtcggcaa
    22981 ctgaccgaac cacgccatgt cgataccatc ctgctggaaa acgggacgct gaatctcacc
    23041 gaccagaccg cgccgctacc gttcaaagcc gatcgtctgc aactgcgtga tatggcgttt
    23101 aatagcccga atagcgaatg gaaactgagc gcgcagcgga taaatggcgg cgtagttccg
    23161 tggtcaccaa aagccggtaa agtgctgggt acgaaggcgc agattcagtt tagtgccgga
    23221 tcgctttcgc tcaatgatgt tcctgccacc aatgtactga ttgaaggcag tattgataac
    23281 gatcgcgtta cgctgactaa cctgggtgcc gacatcgccc gcgggacatt aaccggaaac
    23341 gcgcagcgta acgccgacgg cagctggcaa gtggaaaacc tgcgcatggc ggatatacgt
    23401 ctacaaagcg aaaaatcgct aaccgacttc tttgcgccat tacgctctgt cccgtcgttg
    23461 cagattggtc gcctggaagt gatcgatgct cgtttgcaag gtccggactg ggcggtgacc
    23521 gacctcgatc tcagcttgcg caacatgacc ttcagtaaag atgactggca gacacaggaa
    23581 ggcaaactgt cgatgaacgc tagcgagttc atttatggtt cgctgcattt atttgacccg
    23641 attataaacg cggaattttc cccgcagggc gtagcgctgc gccagttcac cagccgctgg
    23701 gaagggggta tggtcagaac gtcagggaac tggctgcgtg acgggaaaac gttgatcctt
    23761 gatgatgtgg caattgccgg gctggaatat accttgccga aaaactggca acagttgtgg
    23821 atggaaacga cacccggttg gttaaacagc ctgcaactga agagatttag cgccagccgc
    23881 aatctgatca ttgatatcga ccctgacttc ccgtggcagc tcaccgcgct cgatggttac
    23941 ggtgccaacc tgacgctggt taccgatcat aaatggggcg tctggagtgg ctcggcgaat
    24001 ctgaatgccg ccgccgcgac attcaatcgt gttgatgttc gtcgcccgtc gctggcgctg
    24061 accgccaaca gcagcacggt gaatatcagc gaactgagtg catttactga aaaaggcatt
    24121 ctggaagcca ctgccagtgt ttcacaaacg ccacaacgtc agacccatat cagcctgaat
    24181 ggacgcggtg tgccggtgaa tattttgcaa caatggggat ggcctgaatt accgttgact
    24241 ggcgacggca atattcagct taccgccagt ggagatattc aggccaatgc cccgctgaaa
    24301 cctacggtta gcgggcaatt gcatgccgtg aacgccgcaa agcagcaagt gactcaaacc
    24361 atgaatgctg gcatcgtttc cagcggtgaa gttacgtcgg cagagctggt gcagtaaaca
    24421 tcaatctcgc cgggtacgac tcagacgtac ccggcattcc atcaatagat tacaacgtaa
    24481 ttgtcagcgc attcccttga ggcttcacca ccagcccctg ctcactttca gcctgcgaac
    24541 cgtcttgcag accatttact ttcacaacat tgcgcaggca cagtgtccag ttcttcgcct
    24601 cgcccgcacc agtcacagta atcgtgttgc cagtacgtgc tgcttttaaa gtaaagatca
    24661 ccgatccgtc agcagcgggc acttcacaga cggcttcatg cccgtcttgc agattaaaga
    24721 ggtggaatgc cgtgccttcg tgccacacgt aatcgggacg ttgatcgttg ttgcccagcg
    24781 ccagtagagt gttatcacgc acataaacgg gcagactcag gaagccgtgc tgctgtttat
    24841 gccagcgact accgtcgagt tcatcgttgt gccacaggtg tgtccagcga ccttccggca
    24901 ggtagaactg cacatcgccc gcttcagtga acaccggcgc aaccatcacg ttgttgccta
    24961 acatgtattg acggtcaagg taatcacaag ccggatcgtc cgggaactcc atcatcatgg
    25021 cccgcatcat cggcgtaccc cgcgcgttcg cacgcgcagc ttcacgatac agatacggca
    25081 tcatgcggca tttcagttgc gtgaagaagc gcactacatc acaggactca tcatcgtagg
    25141 cccacggcac acgataagat ttgctaccgt gtaaacggct atggctggag agcaaaccaa
    25201 acgcgcacca gcgtttgtaa acgtgcgccg gagcggtatt ttcaaagccg ccgatatcgt
    25261 ggctccagaa gccaaaacct gaaaggccaa tagacaaacc accgcgcagg ctttccgcca
    25321 ttgattcgta gttagcgtaa caatcgccac cccagtgtac cgggaatttc tgcgcaccga
    25381 cggaggccga gcgggcaaac aagacagctt cttcctcacc aacggtgtcc ttgagcacgt
    25441 tccacaccag ttcgttgtag atgtatgcat aatggttatg cattttctgc ggatcggaac
    25501 cgtcaaacca ctgaacatca gttgggatac gttcgccaaa gtcggtctta aagcaatcaa
    25561 cgcccatcgc gaccagacct ttcagtttgt cggcgtacca tttgcaggca tccggattgg
    25621 taaagtcata aatcgccaga cctggctgcc atttatccca ctgccatagc gaaccgtccg
    25681 ggcgtttgag taaatagcct ttctcttgta actctttaaa gacgggggat ttttgaccga
    25741 tataggggtt aatccagacg cagattttca gccctttcgc tttcaggcgg cggatcatcc
    25801 cttccgggtc agggaaagtc agcgggtccc actcaaaatc gcaccactgg aaggctttca
    25861 tccagaaaca gtcaaagtgg aaaacatgca gcggcagatt gcgttccgcc ataccatcga
    25921 taaagctgtt taccgtcgct tcgtcgtagt tggtggtaaa tgaagtggtt agccacaggc
    25981 cgaaggacca cgcgggcggc agcgccggac gaccagtaaa gcgggtataa cgatcaagta
    26041 ccgctttcgg cgtcgggccg tcgataacaa agtattcgag atattcactc tcaacgctga
    26101 actgcacttt ggagactttc tccgatccca cttcaaaaga gacacactgg ggatgattga
    26161 ccagtacccc ataaccacgg ttagtcatgt agaacgggat atttttatac gcctgttcag
    26221 tacttgtgcc gccgtcccgg ttccaggtct ctaccgtctg gccattgcgc accagggcag
    26281 taaagcgctc tcccagaccg taaactgttt cgccaacgcc aagatcaagc cgctcaaaca
    26341 tataattgcg ttgattattc gtgtcctgca cgtagccatt atttttcacc tgactaccgg
    26401 taatacgttc gccgttgcgc agaaaatcca gtgaccagaa ctcacctttg ctgacacgcg
    26461 cgcttaagtt gccactttta aactcagcat aacgttctgt gttttcgatt gtgaccttca
    26521 cgtcctgcaa aatattgagc ggataatgag gaccgttatt cagcgccccc tgaaaatgct
    26581 caatccgcac accgacaata ccttcctgtg gggagaaaaa gcgcagcgta aataaaggcg
    26641 tatcaagctg ccaggtacgt tcacgcacat cacggggggc agcatagacc accatttcat
    26701 taccctgctg ttcaacctcg aatacctgaa gcgggtgaat caaattgagg ccaggttgaa
    26761 tcaaccagtt tccatcgcta attttcattg ccgttcctta gttctgcact tcttgagagg
    26821 tgaattgttg ctggcaacgg cgtttaccct gagccagctg ttccataacg gttttcagat
    26881 tgtgcgtcgt gagtgagtag tagcgtttag cgataatcgc gctcagcaaa taacagatcg
    26941 ccggaacaat cgtgaatagc gcaataatga tgctaatcgt ggcgctgttc tgcgcttttt
    27001 ctgccgcatc atatccgcca taagccagca tccagccgat aagagcgccg ccgaaggcca
    27061 accccagttt gagcacaaac agcgtgccag caaaactgat cccggtcagg cgtttaccat
    27121 tgcaccactc gccgtagtcg acggtatcgg acatcattac ccactggata ggtgtcacca
    27181 gttgatgcaa cacaccaatc acgaagatga agacaaacat agtgatgctg gcctgcatgg
    27241 gaacaaagaa catcgcgagg ctaatcactg ccagcagggc gttcgtccac cagaagatag
    27301 tgactttaca tttccagtcg gtcagaggtt ttgccagtgc ggaaccaatc aggttaccca
    27361 cgcaataagt ggtgagaaaa gcgacaaaca cttccggcgt gcccaaaatc catgtgacgt
    27421 aatacatcat cgccccaccg cgtacgcaca ccgccaggat attgaaaatg gttagtaaac
    27481 cgacaatccg ccactggtcg ttttgccaga tatcacgtaa atcttcccgc atagacgttg
    27541 ttgtaggtgg tgcttcaacg cgttctttag tggtgaagaa acagaatgcc agcatcatga
    27601 atgccaccac ggaaaggacc gcgataccgc cctggaaacc gagtggttta ttatcaccgc
    27661 caattaaatt aaccagtggc atcatcagaa cagtagaaag catgcctccc gcggtcgcca
    27721 gcacaaaacg ccaggattgc agcgagatac gctgagtcgg gtcattggtg attacaccac
    27781 ccaatgcgca gtaagggata ttgacgacgg tataaagtaa ggtaagtagg gtgtaagtaa
    27841 ttgctgcata gatcattttg ccgttcatac tgagatctgg cgtgctatag gccagtacac
    27901 agacgatccc gaatggcagt gcgccaaaca gtacccacgg acgaaattta ccccagcgag
    27961 agcgcgttcg atcggccaac aaccccatgc aaggatcgga aatcgcatcc agtgcacgag
    28021 cgaccaaaaa catggttccg acaaaaccgg caggaatgcc aaaaatatcg gtataaaaga
    28081 acatcatata taacattacg ttatcgaaaa taatgtggct ggcggcgtct cccatgccat
    28141 aaccaatttt ctctttaacg gacaacactt cactcttcat ctgcttttcc ttgatgctat
    28201 aggggtgctg agaccgataa cgcattttgt aaaccatccc tgtgagagcg aatattccga
    28261 tatctggtta ttaatttatg attcttgttt tatgtgatcg tggtagcgtt aatttcgctc
    28321 atatatcatt gtaaaatata ggttttatat gaactataat gctttcgtga taatacgctg
    28381 cgtgtattag gcggaaaaaa ctgatctggg ggatgtagaa actcaaggaa gtagctataa
    28441 tgcgccccgc ctccatgtag caatcgaggc gcggaagatc gtcgtctccg gtgaggcggc
    28501 tggacttcaa atccagttgg ggccgccagc ggtcccgggc aggttcgact cctgtgatct
    28561 tccgccaaaa tgcctcttct gacgttctcc aaccccttgt aatactgtca atccagatat
    28621 tttctactgc tcaatattca atcggcttca ggaatacctg gtctaaggtt ttgtgatgga
    28681 cgatgagcgg ctgaagaatc cgcctgtcgg ttcttctgtc ctcgaacgca tccgcgatat
    28741 gcgcgccagc atgtgccgtg tttatttgca ggtgatttgc cccctgctgg ctctcagcca
    28801 actgtatcgc cattaaggtg aagatgttgt tttactcgcc ccagaacacc cttttttata
    28861 cgactattct atttctttaa ccgctggcga aaaaaactga ctataaactt c
//
LOCUS       DAZFRJ010000045        28641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000045 DAZFRJ010000000
VERSION     DAZFRJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.045"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHKUN_001761"
     CDS             complement(<1..596)
                     /locus_tag="ACHKUN_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6660029.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKUN_001762"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKUN_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO6660030.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKUN_001763"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKUN_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO6660031.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKUN_001764"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKUN_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO6660032.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKUN_001765"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKUN_001765"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO6660033.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKUN_001766"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKUN_001766"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO6660034.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKUN_001767"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKUN_001767"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO6660035.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKUN_001768"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKUN_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6660036.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKUN_001769"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKUN_001769"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6660037.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKUN_001770"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKUN_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO6660038.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKUN_001771"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKUN_001771"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6660039.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKUN_001772"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKUN_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO6660040.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKUN_001773"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKUN_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO6660041.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKUN_001774"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKUN_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO6660042.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKUN_001775"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKUN_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6660043.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKUN_001776"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKUN_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6660044.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKUN_001777"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKUN_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO6660045.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKUN_001778"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKUN_001778"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO6660046.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKUN_001779"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKUN_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO6660047.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHKUN_001780"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHKUN_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO6660048.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKUN_001781"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKUN_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO6660049.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKUN_001782"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKUN_001782"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO6660050.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKUN_001783"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKUN_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO6660051.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKUN_001784"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKUN_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO6660052.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKUN_001785"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKUN_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO6660053.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKUN_001786"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKUN_001786"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO6660054.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKUN_001787"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKUN_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO6660055.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKUN_001788"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKUN_001788"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO6660056.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKUN_001789"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKUN_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO6660057.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKUN_001790"
     CDS             26270..27076
                     /gene="nirC"
                     /locus_tag="ACHKUN_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO6660058.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKUN_001791"
     CDS             27095..28468
                     /gene="cysG"
                     /locus_tag="ACHKUN_001791"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO6660059.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6908 a         7709 c         7245 g         6779 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26281 cactattaat aagtgtgcgg ctaatgctgc acgcattgca cgcctgtcgg caaataaccc
    26341 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    26401 gattttcacg ctcagtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    26461 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26521 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    26581 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26641 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26701 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26761 ttgcctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    26821 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tctatcgcta acatgacgct
    26881 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26941 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    27001 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27061 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27121 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27181 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27241 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27301 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacacgctta accagcgcgt
    27361 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta gtcgatgcgc cgaaagccgc
    27421 cagctttatt atgccgtcga ttattgaccg ctcaccgcta atggtagcgg tctcctctgg
    27481 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    27541 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    27601 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    27661 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    27721 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27781 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27841 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    27901 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    27961 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    28021 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28081 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccgctcacgc atcgcgatta
    28141 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28201 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28261 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28321 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28381 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28441 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    28501 aatgactctt tttatatatt cagcaaataa aacatagccc cttaataaat attatctgct
    28561 aatgggttat attcaccgtt acagcgactt acaaaattaa gtcattcgct cactgacgcc
    28621 aattaaatat tcctgtatcg t
//
LOCUS       DAZFRJ010000046        28336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000046 DAZFRJ010000000
VERSION     DAZFRJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.046"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHKUN_001792"
     CDS             complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHKUN_001792"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO6660060.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHKUN_001793"
     CDS             complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHKUN_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6660061.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAISDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHKUN_001794"
                     /pseudo
     CDS             complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHKUN_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4668..5747
                     /gene="frwC"
                     /locus_tag="ACHKUN_001795"
     CDS             4668..5747
                     /gene="frwC"
                     /locus_tag="ACHKUN_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO6660062.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5762..6082
                     /gene="frwB"
                     /locus_tag="ACHKUN_001796"
     CDS             5762..6082
                     /gene="frwB"
                     /locus_tag="ACHKUN_001796"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6660063.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6133..8430
                     /gene="pflD"
                     /locus_tag="ACHKUN_001797"
     CDS             6133..8430
                     /gene="pflD"
                     /locus_tag="ACHKUN_001797"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6660064.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8396..9274
                     /gene="pflC"
                     /locus_tag="ACHKUN_001798"
     CDS             8396..9274
                     /gene="pflC"
                     /locus_tag="ACHKUN_001798"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO6660065.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9276..9617
                     /gene="frwD"
                     /locus_tag="ACHKUN_001799"
     CDS             9276..9617
                     /gene="frwD"
                     /locus_tag="ACHKUN_001799"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6660066.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHKUN_001800"
     CDS             complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHKUN_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6660067.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHKUN_001801"
     CDS             complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHKUN_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6660068.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHKUN_001802"
     CDS             complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHKUN_001802"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO6660069.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHKUN_001803"
     CDS             complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHKUN_001803"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO6660070.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16828..17832
                     /gene="argC"
                     /locus_tag="ACHKUN_001804"
     CDS             16828..17832
                     /gene="argC"
                     /locus_tag="ACHKUN_001804"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO6660071.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17840..18616
                     /gene="argB"
                     /locus_tag="ACHKUN_001805"
     CDS             17840..18616
                     /gene="argB"
                     /locus_tag="ACHKUN_001805"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO6660072.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18677..20050
                     /gene="argH"
                     /locus_tag="ACHKUN_001806"
     CDS             18677..20050
                     /gene="argH"
                     /locus_tag="ACHKUN_001806"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO6660073.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHKUN_001807"
     CDS             20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHKUN_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO6660074.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHKUN_001808"
     CDS             complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHKUN_001808"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO6660075.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22948..23598
                     /gene="fabR"
                     /locus_tag="ACHKUN_001809"
     CDS             22948..23598
                     /gene="fabR"
                     /locus_tag="ACHKUN_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO6660076.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23598..23957
                     /gene="yijD"
                     /locus_tag="ACHKUN_001810"
     CDS             23598..23957
                     /gene="yijD"
                     /locus_tag="ACHKUN_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO6660077.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHKUN_001811"
     CDS             complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHKUN_001811"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO6660078.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25211..25401
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25466..27310
                     /gene="btuB"
                     /locus_tag="ACHKUN_001812"
     CDS             25466..27310
                     /gene="btuB"
                     /locus_tag="ACHKUN_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO6660079.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27255..28112
                     /gene="murI"
                     /locus_tag="ACHKUN_001813"
     CDS             27255..28112
                     /gene="murI"
                     /locus_tag="ACHKUN_001813"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO6660080.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6479 a         7125 c         7814 g         6918 t
ORIGIN      
        1 actgataagc gtagcgcatc aggctatttt gcgttcaaac tcccggacaa gccgggagtt
       61 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag
      121 agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
      181 tgcacatgcc gcttctgcca caattcgcat tttcgccggg acatcttctt taatatccag
      241 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
      301 ctcaaccggc gcgttttcca gtaccagctg cgtcagcgta ccgaatgcca ctttttcacc
      361 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg catgagccgc
      421 cgccagtccg ccgctttcaa aaccaacgcc gcttaaatag gtgttcgctt caatcacgcg
      481 ttccagcgca ggggttacta catgctgttc ggcggcaagc atcgcttttt cgccttcttc
      541 cagcagagtg ttgtagcaca gttctgccag tgccagcgcc gcctgggtgc acttgccgcc
      601 cgccatagtg gtagcgccgc tacgggaaca ggcgcgtgct tcaaaccagg ttgccagcgc
      661 atcgccgata cccgccgcta acagacgtgc aggagcgccc gcgacgattt tggtgtcgac
      721 aatgaccata ttcgggttat ttggcaacag tagatagcgg tcaaactcac cctcatcggt
      781 gtagataaca gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc
      841 taccggaaca cccatgaaat gtgccagtgc tttggcagta tcgagggttt ttccgccacc
      901 gataccgaga attgcgccac actgcgcagt ctccgcgatg ccacgcagac gatcgatctc
      961 attttgcgaa cattcaccgc caaacggcgc aatttctact accagtccag catctttaaa
     1021 gcttttctcg acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca
     1081 gcgttctgcc agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat
     1141 gtatttaccc ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc
     1201 caaatgcggc attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt
     1261 tgagcatttg ttgcgctaca tctaaaggca gggtgatgga ctcacatcct gccagcaaac
     1321 aatccagtgc ctgacgcggc gttttaaagc tggctgcaag caccatgctt tctggcgcgt
     1381 gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat gccgtccccg ccctgggcat
     1441 ctacgcggtt aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc agtaaccctt
     1501 gtgcagcact gtacactgcg gtgccaagcg tagtaatacc ctcttttttc agcatcttaa
     1561 tggcggccag accttcggaa gtcaccggga ttttcaccac aatgcccgga atggcgtcgc
     1621 gcaggcgctt cgcttcttcc accataccct gcgcgtcacg actcatagtc tgagcaaaca
     1681 gaatgccctc atcactaatc gctttttgca gacgcggcag aacttcccat atggactcct
     1741 tgctggcagc gataatgctc gggttagttg tcaccccggc aatggggaat atgcgtgcca
     1801 gacgttcgac ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta
     1861 cagttccagt tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag
     1921 cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg
     1981 ctgatcgccc gcagcgtgtt tgttcagggt taacataatg atgaattgcg cttcatcatc
     2041 gccccagcgc accggcgctt gcagacgcgc tacactgatg gtggattgct caatgtgttc
     2101 tgatttgctg tggggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg
     2161 cgcccacaag tcggcttcca gtttacgcgg atagcggcag cggcccgcca gcagcaggtt
     2221 atcggtcatg cctttgagca cttcttcttt gctgcgtcag tcgctttcca gcgtgatgca
     2281 ctcggcggtg accagcggtg cgtcctgttg ggtcatgcgg aattgcgcca gcaggtgttc
     2341 cacttccagc gaagtacggc aggccattgc ctggttgagc aactggcggc attcgcggct
     2401 atcaagttgc gccattcgcg ctttcgccgc cggaattgac ggtgcgctca tgctcagctc
     2461 atccagccct aaaccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca
     2521 cagaccaatc catttgccct ggcgatgcac cgcttgcacg gcgtaatcga gcgcccgcaa
     2581 gaatgccgga ttcaggctgt tgtagtgacg agtaacctta gcattatcgc gatccaccgc
     2641 cagcagatat tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca
     2701 ttgatcgatg atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc
     2761 atcaaacgga atgtgttcgt tacgcagttg ctgtttggct tccgccagtt tttctttcac
     2821 ccataagatc tcttccattg aagagatcat cgggatcatg attttcaggc tgccgtgagc
     2881 ggaggcgcgc aggatcgacc gtagttgtgt ggtgaacagc gaggcgtatt cttcataaat
     2941 acgcacagcg cgataaccga ggaacgggtt agcttctgcc ggaatgttca gataatcaac
     3001 gggtttgtcg ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc
     3061 cagcgcctga caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat
     3121 atagagcatt tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg
     3181 cgcttccacg gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg
     3241 ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg gcatcctgta cgcgggcttc
     3301 ttgctgataa taacgggcta cggcttcccc tggctcaacc acaatcgctc cggcgttgcc
     3361 gtcgatataa atcgtttgat gttgccacgg ggtaagggca tcaatatcca caccaaccag
     3421 cgtcggaatg ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccgctttt
     3481 gagcaacaat cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc
     3541 atcagccata caaatggcgg gctgcgtcag tttgcccggt gccgggaagc gttgctcacc
     3601 gtagatttgc tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag
     3661 gtagctgctg ctggaacggg aaaactcttc acaaaagtga ttcgcactgg cgacaatcgc
     3721 ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa tgttcgtgca gggaagtatc
     3781 gccagccagg gatcggtgcg cttccagaat cgcgctggtg gcaccgtcgc tatccagcag
     3841 acgaaactca atgtttttca gtaccagcgt caggccgttt tccagtgcgg attgctcggc
     3901 gtcaacgtct ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg
     3961 cgtcagaatg ccgcctgcgc taccgctgca cacggtgcgg gcgcggataa tttgcggatt
     4021 cagattggtc agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg
     4081 tgcgtcgcag tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg
     4141 ttcgtcggcc ccggaaatca gtaaccggca gttatcgccc gccagcgtgt cggtgccaat
     4201 cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg cgcaggttat gccactcaat
     4261 ttgtgatgaa aaagtattac acagcgtttc aacgtggctt gccggacgtg catgtacgcc
     4321 gttaggtagc tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt
     4381 ttctgtttac aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag
     4441 ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg
     4501 catgcttccg gattatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa
     4561 agtccagtaa atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc
     4621 tccatcaaaa atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg
     4681 tgcagatcct gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct
     4741 tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag
     4801 tgccggatgc cgtagccgat ccgaatctga aaaaactgtt tgatatcgga gttgcaggtt
     4861 tgacgctgat ggtgcctttc ctcgccgctt acatcggtta ctccattgca gagcgttctg
     4921 cgctggcccc gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg ggcttctttg
     4981 gtgcgctgat cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg
     5041 tgcataaagt tctgcgctcg gtgatgccta tcttcattat tcctatcgtc ggcacactga
     5101 ttaccgcagg catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc
     5161 tgactcagtg gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg
     5221 gtctgatgct ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc
     5281 tgatttgcgt tgctcagggt gtttataccg tggtggccat tgctgccgtg ggtatttgta
     5341 tcccgccgct ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag
     5401 agcgcgaaac cggcaaagcg gcgctggtga tggggtgcgt tggggttact gaaggggcga
     5461 ttcctttcgc cgctgccgat ccgctgcgtg tcattccttc catcatggtc ggttctgttt
     5521 gtggtgcggt aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga
     5581 ttgtgctgcc ggtggttgaa ggcaagctgg gttatatcgc agcagtggct gtcggagcag
     5641 tggtgacggc tgtttgcgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga
     5701 ctgatgagaa ggaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc
     5761 catgacgaaa attattgcag taaccgcgtg tccatctggt gttgcccata cctatatggc
     5821 ggcagaggcg ctggaaagcg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaactca
     5881 ggggtcaatt ggtctggaaa acgaactgac tgcggaagac gtggcgagcg ccgatatggt
     5941 gattctgacc aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt
     6001 gcgcgtcaac atcagcgatg cggtaaaacg tgctgacgcc attatgagca aaattgaagc
     6061 tcacctggcg caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca
     6121 caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt ttccaatacc
     6181 cgtgaaatct cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc
     6241 gaaccggtga tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt
     6301 tcgattcgtg atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt
     6361 atgtcgccgg aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt
     6421 ccgcaggacc gctttgctat cagcgaagaa gataaacgta tctaccgcga agagttgttc
     6481 ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta
     6541 aaagccgcga ccagcacgca gattttcagc attaaccaga cggataaagg ccaggggcac
     6601 attattattg attacccacg cctgctgaat cacgggctgg gggagctggt ggcgcagatg
     6661 cagcaacatt gtcagcaaca gccggagaat cacttttatc aggccgcgct gttactgctg
     6721 gaagcctcgc agaaacacat tctgcgttac gctgaactgg cggaaacaat ggcggcaaac
     6781 tgcacagatg cccagcgtcg cgaagaactg ctgactattg cggagatctc ccgccataac
     6841 gccgaacata agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc
     6901 attctgcaat acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg
     6961 ttgccgttct atcaggcttc tttaacccag ggcgaagatc cggcgttcct gaaagaactg
     7021 ctcgaatctt tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagcagcgcg
     7081 cgttatttcg caggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac
     7141 ggacgtagcg cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa
     7201 ttaccgcaac cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg
     7261 aaaaccgccg aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg
     7321 gtggtaccag cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta
     7381 gtgggctgtg tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg
     7441 tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg
     7501 acttatgaag gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg
     7561 gttgagggca gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc
     7621 tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt
     7681 tataacttct ccggcgtaca ggggatcggt atcgccaacc tgagcgattc tctccatgcg
     7741 ttgaaaggga tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta
     7801 aaagccaact ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat taaccgcttc
     7861 gagaaatacg gtaacgatat cgacgaggtg gataacatca gcgccgaact gttacgccac
     7921 tactgcaaag aagtggaaaa ataccagaac ccgcgcggtg gttacttcac gccgggatcg
     7981 tataccgttt ctgctcacgt cccgttggga tcggtggttg gcgcgacgcc agacggtcgt
     8041 tttgccgggg aacaactggc agacggcggc ttgtcaccta tgctgggcca ggacgcacaa
     8101 gggccaacag cggtactgaa gtcagtcagt aagctcgata atacgctgct gtctaacggt
     8161 acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg attacgcaaa
     8221 ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca gtttaacgtg
     8281 gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc cgggctggtg
     8341 gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat ccaggatgac
     8401 atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca atgatgtggc
     8461 gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc gtacggtggt
     8521 cttttttaaa ggctgtccgc atctttgccc atggtgtgct aatccggagt cgatctccgg
     8581 caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat gtttgcgtga
     8641 tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca gccttgacgc
     8701 gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg gcggcgtcac
     8761 gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt tacagcggct
     8821 gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgtgccag ccagcaagct
     8881 gttaccactg gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta tggacgcgac
     8941 tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc tgcgtttgct
     9001 ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt tcacgctcag
     9061 ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca ggcagatcca
     9121 tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga aaacatggtc
     9181 gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg aaatggcaga
     9241 acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg gcagtaaccg
     9301 cctgcgtaag cggcgtggcg catacttata tggcggcgga acggctggaa aagttgtgcc
     9361 agttagagaa gtggggcgtt agcattgaaa ctcagggcgc gctgggaacg gagaatcgtt
     9421 tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat atcgagcttg
     9481 ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac gcattcctgc
     9541 gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg cagcaaaccc
     9601 atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc gacgatattc
     9661 cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc tgtcgacaaa
     9721 gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata acgtcttagc
     9781 gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg ttttttgaaa
     9841 caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt cgcgggtaag
     9901 cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcaaatagtg cctggctgcg
     9961 tgaggcggtc tggatttggc tgccgagcaa atcgcggcag tggctaagca ggctggcgac
    10021 gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga gcgtttgtag
    10081 cagaaaagaa cctatgcgtg ggccgcgccg ggcgacgtgt tgcttcgcca gattttgata
    10141 ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa acagcacgct
    10201 aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcag gaacgtacag
    10261 cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac cttccagcac
    10321 aatttccagc cgtggaaaat cgacctgata cgcgagatcg ggcaccgggc cgttactgct
    10381 ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga gcagatagct
    10441 gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta actggcatta
    10501 ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac attgcggcag
    10561 tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga ttacccacct
    10621 gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa
    10681 tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt
    10741 cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac
    10801 gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc gacgtccaca
    10861 gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt
    10921 tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc aggaaaccgt
    10981 tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt
    11041 tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca ggcggaacat
    11101 gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat
    11161 gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc agcacttcct
    11221 ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca
    11281 tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg cgggcggtca
    11341 tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc atgttcatca
    11401 gcgacggttg tgtcaggtac aggtccgggt tcttttcatt agcaaaggtc agcgcctgtt
    11461 gcaggatttc aatggtgtac ggacgagacg taaccacgtt attaaacacg gtcagattcg
    11521 gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac
    11581 tcatgcgccc gcgttgggtc gattcgccaa tcaccagcac taaggtacgc ggttcgttac
    11641 ccgattcatc tttgaaatta gccagaggcg gcaaggcatt attttcattc agtaactttg
    11701 ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg
    11761 caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg tttttgataa
    11821 acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat
    11881 aacgccacgg ctttggaata tagaccgggt gcaggcgtgt ccacagcaga actgccaccg
    11941 ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc
    12001 tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc
    12061 cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc acgccgatta
    12121 ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc
    12181 tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg
    12241 cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc
    12301 taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa
    12361 atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa
    12421 taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa
    12481 ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc
    12541 tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg
    12601 gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata cgttcagcgg
    12661 gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat cggccattag
    12721 atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa
    12781 ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt attccgccag
    12841 ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg
    12901 ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag
    12961 cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat
    13021 ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga
    13081 acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa
    13141 atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc
    13201 cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat
    13261 agccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg
    13321 gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga
    13381 cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa
    13441 caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata
    13501 ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat agccaatcat
    13561 caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca gctgggtcat
    13621 gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa ccggcatcgc
    13681 aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt
    13741 cgccatcgag atcacgtagg cggcaatgga gccttgcggt gcttcggcaa tcacctggca
    13801 ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg
    13861 tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta
    13921 gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatgac gcgtgctctc
    13981 ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc
    14041 gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc
    14101 gtagagcggt tcccacagtt cttcgttttg tgtcagcagg ccttctggtt ttggcagttc
    14161 ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt
    14221 tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc
    14281 aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt tcaggaacaa
    14341 atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt
    14401 cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa caaattcgac
    14461 gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca ggtaatttgg
    14521 tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt catctaccgg
    14581 gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta actggcgcag
    14641 gcggcgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa
    14701 acaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg
    14761 agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct
    14821 cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg ggttgctggc
    14881 agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa
    14941 ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt
    15001 gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat
    15061 agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac
    15121 tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca
    15181 gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg
    15241 ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc
    15301 tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg
    15361 ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg
    15421 taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcacag gagtttggca
    15481 acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc agcaaaaatg
    15541 gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt ccagatattc
    15601 atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg ggcataacgt
    15661 ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc cgagcaattt
    15721 ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg
    15781 cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt
    15841 gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa
    15901 ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag
    15961 cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa
    16021 ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg
    16081 atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg
    16141 tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc
    16201 ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag
    16261 aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa
    16321 cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt
    16381 cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc
    16441 cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga atttgttgcg
    16501 agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc agtccgccag
    16561 cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc ttattgaagg
    16621 tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt tcattgttga
    16681 cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta
    16741 acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa
    16801 ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc
    16861 tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct
    16921 ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag
    16981 ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga gtttagccca
    17041 ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag
    17101 tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc
    17161 accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt ggaacaggca
    17221 gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg
    17281 ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt
    17341 cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt
    17401 aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttacc
    17461 catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt caccccgcat
    17521 ctgggcaatt tcccgcgcgg cattcttgaa accattacct gccgcctgaa accgggtgtg
    17581 acccaggcgc aagtggcgca agcgttacag caggcgtatg cccataaacc gctggtgcgg
    17641 ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatg ttgcgatatc
    17701 gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa
    17761 ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag
    17821 tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct
    17881 ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca
    17941 tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg
    18001 gctgaatctg ccggtgaaaa agaaaaatgg cctgcgggtg acgcctgcgg atcagataga
    18061 cattatcacc ggagcactgg caggaacggc aaataaaacc ctgctggcat gggcgaagaa
    18121 acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca
    18181 gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa
    18241 caccttgctg gagaacggct atctgccggt ggtcagctcc attggcgtaa cagacgaagg
    18301 gcaactgatg aacgtcaatg ccgaccaggc ggcgacggcg ctggcggcaa cgctgggcgc
    18361 ggatctgatt ttgctctctg atgtcagcgg cattctcgac ggcaaagggc aacgcattgc
    18421 cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat
    18481 gatagtgaaa gtgaacgcgg cgctggatgc tgcgcgcacg ttgggccgtc cggtagatat
    18541 cgcctcctgg cgtcatgcgg aacagcttcc ggcactgttt aacggtatgc cgatgggtac
    18601 gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt gaatttcaaa
    18661 ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg
    18721 gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt
    18781 tggctctgtg gcctggtcca aagccctggt aacggtcggc gtgttaaccg cagaagagca
    18841 ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca
    18901 aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa
    18961 agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc aggtagcgac
    19021 agacctgaaa ctgtggtgca aagataccgt tggcgagttg ctgacggcta accggcagct
    19081 gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtgatgc caggttacac
    19141 tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat
    19201 gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt
    19261 aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg
    19321 gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc gtgaccatgt
    19381 gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga
    19441 tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc
    19501 cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa
    19561 atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag gtttgccgct
    19621 ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg
    19681 gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga aacgtccacg
    19741 ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg attatctggt
    19801 ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc
    19861 cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga aattcagccc
    19921 ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc
    19981 ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg
    20041 gttggggtaa gaacatttat atgtataaat ttgagcctgg cttatcgccg ggctttttta
    20101 tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
    20161 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
    20221 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
    20281 atcgccatga actatcgtgg caatggagga tggataatga atattcgtga tcttgagtac
    20341 ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
    20401 cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt gatgttgctg
    20461 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt agatcaggcg
    20521 cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggcgagacg
    20581 atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct gctaccgcat
    20641 attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag
    20701 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
    20761 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
    20821 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg
    20881 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgt
    20941 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga gacgctgcgc
    21001 aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt gccgccggag
    21061 cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt
    21121 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
    21181 atccgcgcac gaatggatgg ccatttcgat aaagttttaa aacaggcggt ttaaaccgtt
    21241 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
    21301 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
    21361 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
    21421 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa
    21481 ctgggcgcgg cccacttcat atggcacttt cattgcggtc agctgctgtt cggttttgcc
    21541 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
    21601 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
    21661 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
    21721 atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa tgttctgtaa
    21781 cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt cagctttcag
    21841 ttttttaccc gacttcaaat gcatgatcac accatcgtca cagccttcga tcttctcgta
    21901 ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga gagaatctga
    21961 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
    22021 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
    22081 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
    22141 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
    22201 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
    22261 aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
    22321 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
    22381 gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa tgcggctgac
    22441 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
    22501 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
    22561 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
    22621 agggcttacc tgttcttata cataaaagca acagaatggt aacattttat cgcgggtaag
    22681 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
    22741 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
    22801 ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt tttattgcgt
    22861 taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc
    22921 agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa
    22981 acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcacc
    23041 agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg
    23101 catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta
    23161 cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc
    23221 tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg gttattattg
    23281 cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac
    23341 ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact
    23401 gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac
    23461 gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt
    23521 tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga
    23581 aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt
    23641 tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat
    23701 tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc
    23761 ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg
    23821 tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc
    23881 cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc
    23941 aggaagttgc tgaataatcg gtattatgcc ggatgctggc gcatccagca tgggttttac
    24001 ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga
    24061 gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca taacgtttcc
    24121 gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc ggtttcactg
    24181 tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact ctttaagtcg
    24241 atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc
    24301 gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta ttgcgcagca
    24361 gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct
    24421 aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt
    24481 acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg
    24541 ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa
    24601 tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc
    24661 tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc atccagcttc
    24721 ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg
    24781 aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac
    24841 tggttgataa gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt
    24901 tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc
    24961 atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc
    25021 atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga
    25081 aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt
    25141 gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac
    25201 ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga
    25261 atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc
    25321 catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct
    25381 gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc
    25441 cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc
    25501 tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc
    25561 taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca
    25621 ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt
    25681 cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa
    25741 tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg gggtgagtgg
    25801 ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat atatccgtgg
    25861 gccacgctcc gccgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac
    25921 gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta
    25981 tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt tgggcgatta
    26041 tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac
    26101 agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata actttactga
    26161 tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt atgacgcgta
    26221 ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc
    26281 cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct atagccatag
    26341 caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga
    26401 gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg
    26461 tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg ttgagaatgg
    26521 atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac
    26581 ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg gaacctggca
    26641 aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc
    26701 ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc
    26761 ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg
    26821 tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa
    26881 atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca attttgatac
    26941 cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga
    27001 cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta
    27061 tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata aggattactc
    27121 atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc
    27181 gtatccggtc acctctcatc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga
    27241 ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta
    27301 caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg
    27361 tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg
    27421 tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg gtggcaattg
    27481 tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca
    27541 gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc
    27601 cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc
    27661 gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc
    27721 agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag cttcatggcg
    27781 aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc
    27841 cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa gaactcttac
    27901 aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct cgcagaacgg
    27961 cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat attgcctttt
    28021 gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg
    28081 aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcagaaaa
    28141 ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca
    28201 cggaacaacg gcaaacacgt cgccgggtca gcggggttct cctgagaatc tcaacagaga
    28261 aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcgacgctg
    28321 agaaaaagcg aagcgg
//
LOCUS       DAZFRJ010000047        28234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000047 DAZFRJ010000000
VERSION     DAZFRJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.047"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            387..1526
                     /gene="wza"
                     /locus_tag="ACHKUN_001814"
     CDS             387..1526
                     /gene="wza"
                     /locus_tag="ACHKUN_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO6660081.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1532..1975
                     /gene="wzb"
                     /locus_tag="ACHKUN_001815"
     CDS             1532..1975
                     /gene="wzb"
                     /locus_tag="ACHKUN_001815"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO6660082.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1978..4140
                     /gene="wzc"
                     /locus_tag="ACHKUN_001816"
     CDS             1978..4140
                     /gene="wzc"
                     /locus_tag="ACHKUN_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO6660083.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4233..5072
                     /gene="wcaA"
                     /locus_tag="ACHKUN_001817"
     CDS             4233..5072
                     /gene="wcaA"
                     /locus_tag="ACHKUN_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO6660084.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5075..5563
                     /gene="wcaB"
                     /locus_tag="ACHKUN_001818"
     CDS             5075..5563
                     /gene="wcaB"
                     /locus_tag="ACHKUN_001818"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO6660085.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5560..6777
                     /gene="wcaC"
                     /locus_tag="ACHKUN_001819"
     CDS             5560..6777
                     /gene="wcaC"
                     /locus_tag="ACHKUN_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO6660086.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6752..7969
                     /gene="wcaD"
                     /locus_tag="ACHKUN_001820"
     CDS             6752..7969
                     /gene="wcaD"
                     /locus_tag="ACHKUN_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO6660087.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7980..8726
                     /gene="wcaE"
                     /locus_tag="ACHKUN_001821"
     CDS             7980..8726
                     /gene="wcaE"
                     /locus_tag="ACHKUN_001821"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO6660088.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8742..9290
                     /gene="wcaF"
                     /locus_tag="ACHKUN_001822"
     CDS             8742..9290
                     /gene="wcaF"
                     /locus_tag="ACHKUN_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO6660089.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9316..10437
                     /gene="gmd"
                     /locus_tag="ACHKUN_001823"
     CDS             9316..10437
                     /gene="gmd"
                     /locus_tag="ACHKUN_001823"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO6660090.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10440..11405
                     /gene="fcl"
                     /locus_tag="ACHKUN_001824"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10440..11405
                     /gene="fcl"
                     /locus_tag="ACHKUN_001824"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO6660091.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11408..11887
                     /gene="gmm"
                     /locus_tag="ACHKUN_001825"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11408..11887
                     /gene="gmm"
                     /locus_tag="ACHKUN_001825"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6660092.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11884..13107
                     /gene="wcaI"
                     /locus_tag="ACHKUN_001826"
     CDS             11884..13107
                     /gene="wcaI"
                     /locus_tag="ACHKUN_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO6660093.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13110..14546
                     /gene="cpsB"
                     /locus_tag="ACHKUN_001827"
     CDS             13110..14546
                     /gene="cpsB"
                     /locus_tag="ACHKUN_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO6660094.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPTGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14739..16109
                     /gene="cpsG"
                     /locus_tag="ACHKUN_001828"
                     /gene_synonym="manB"
     CDS             14739..16109
                     /gene="cpsG"
                     /locus_tag="ACHKUN_001828"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6660095.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16245..17639
                     /gene="wcaJ"
                     /locus_tag="ACHKUN_001829"
     CDS             16245..17639
                     /gene="wcaJ"
                     /locus_tag="ACHKUN_001829"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO6660096.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17641..19119
                     /gene="wzxC"
                     /locus_tag="ACHKUN_001830"
     CDS             17641..19119
                     /gene="wzxC"
                     /locus_tag="ACHKUN_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO6660097.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19265..20545
                     /gene="wcaK"
                     /locus_tag="ACHKUN_001831"
     CDS             19265..20545
                     /gene="wcaK"
                     /locus_tag="ACHKUN_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO6660098.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            20542..21762
                     /gene="wcaL"
                     /locus_tag="ACHKUN_001832"
     CDS             20542..21762
                     /gene="wcaL"
                     /locus_tag="ACHKUN_001832"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO6660099.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21773..23167
                     /gene="wcaM"
                     /locus_tag="ACHKUN_001833"
     CDS             21773..23167
                     /gene="wcaM"
                     /locus_tag="ACHKUN_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO6660100.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23342..24235
                     /gene="galF"
                     /locus_tag="ACHKUN_001834"
     CDS             23342..24235
                     /gene="galF"
                     /locus_tag="ACHKUN_001834"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO6660101.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            24607..25692
                     /gene="rfbB"
                     /locus_tag="ACHKUN_001835"
     CDS             24607..25692
                     /gene="rfbB"
                     /locus_tag="ACHKUN_001835"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6660102.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            25692..26591
                     /gene="rfbD"
                     /locus_tag="ACHKUN_001836"
     CDS             25692..26591
                     /gene="rfbD"
                     /locus_tag="ACHKUN_001836"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO6660103.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26649..27527
                     /gene="rfbA"
                     /locus_tag="ACHKUN_001837"
     CDS             26649..27527
                     /gene="rfbA"
                     /locus_tag="ACHKUN_001837"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6660104.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27532..28074
                     /gene="rfbC"
                     /locus_tag="ACHKUN_001838"
     CDS             27532..28074
                     /gene="rfbC"
                     /locus_tag="ACHKUN_001838"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO6660105.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKGSSILRLNDY"
BASE COUNT         7074 a         6821 c         7560 g         6779 t
ORIGIN      
        1 aattgttacc ttgcctgcta ttccgttagc tgtaacactt cctcctgcat tattggagag
       61 ccaatattca attaccataa tgtcacctta tattcggaag ttaatttccg caataaccct
      121 ttctcatcaa cgattgtatg cagagagggt ttgaaaaatt aagctaaatc agtgtattgg
      181 tagctaaaaa gccaggggcg gtagcgtgtc tggattcctg aaagaccagt ctgaattatt
      241 ctgtcaatag cctgcggatg aaatcaattt ttttaggact gatgccagtt aaatttttat
      301 tcaattaact gcaaggtaaa tgtgcatggc gacattgcta ttaatagtgc acaggataat
      361 tactctgcca aagtgataaa taaacaatga tgaaatccaa aatgaaattg atgccattat
      421 tggtgtcagt aaccttgata agcggttgca cagtacttcc gggcagcaat atgtcgacga
      481 tgggcaaaga cgtcatcaaa cagcaggacg ctgatttcga tctcgacaaa atggtgaatg
      541 tttatccgct gaccccgcgc ctgattgacc aattacgccc acgcccgaat gtagcgcgcc
      601 ccaatatgac gctggaaagc gagatcgcga attatcagta tcgcgtcggg ccgggggacg
      661 ttcttaatgt caccgtctgg gatcacccgg aactcaccac gccagccggt cagtaccgca
      721 gctccagcga caccggcaac tgggtacagc ctgacggcac tatgttttac ccgtatatcg
      781 gcaaggttca cgtagtcggg aaaacgctcg ctgaaatccg cagtgatatt accgggcgct
      841 tagcgacgta catcgctgac ccgcaggtgg acgttaatat cgccgccttc cgctcgcaaa
      901 aggcctatat ctcaggtcag gtgaataaat ccggtcaaca ggcgatcacc aacgtaccac
      961 tgaccattct cgacgccatc aacgccgcag gtggcctgac cgacaccgct gactggcgca
     1021 acgtggtgct aacacacaat ggtcgtgaag aacgcatttc tttgcaggcg ctgatgcaaa
     1081 acggcgacct caatcagaac cgactgcttt atcccggcga tattctctac gtgccgcgta
     1141 atgatgatct gaaagtattt gtgatgggtg aagtgaagaa acagagcacc ctgaaaatgg
     1201 actttagcgg catgaccctg actgaagctc tgggcaatgc cgaaggcatc gacatgacca
     1261 cctccaacgc cagcggcatc tttgtcattc gtccgttgaa aggcgagggc gggcgtaacg
     1321 gcaagattgc caatatctac cagctggata tgtccgatgc gacgtcgctg gtgatggcga
     1381 cagaattccg cctgcaacct tatgacgtgg tgtatgtcac caccgccccg gtttcccgct
     1441 ggaaccgtct gatcaatcag ttgctgccaa ctattagcgg tgtccgttac atgacggata
     1501 cagccagcga cattcataac tggtaatcgt catgtttaac aacatcttag ttgtctgtgt
     1561 cggcaatatt tgccgttccc cgacggcgga acgcttactg caacgttatc acccggagct
     1621 gaaagtggag tccgctggac tcggcgcgct ggtcggtaag ggcgctgatc ctaccgctat
     1681 cagcgtcgcc gcagaacatc aactgtctct ggaaggtcac tgtgcacggc aaatcagccg
     1741 ccgcctgtgt cgcaactatg acctgatttt gaccatggaa aagcgccata tcgaacgctt
     1801 atgcgagatg gcacctgaga tgcgcggcaa agtgatgctg tttggtcact gggataacga
     1861 atgtgaaatc cccgatccgt atcgcaaaag ccgggaaacg tttgcagcgg tgtacacatt
     1921 acttgaacgg tctgcccgcc agtgggcgca ggcattgaac gcagagcagg tataagaatg
     1981 acagaaaaag taaaacaaca tgccgctccg gtaacgggca gtgatgaaat cgatattggt
     2041 cgcctggtcg gcaccgtcat tgaagcgcgc tggtgggtga ttggcatcac cgctgtattt
     2101 gccctctgtg ctgtggttta cactttcttc gccacgccga tttatagtgc cgacgcactg
     2161 gtacaaatcg aacaaaacag cggcaattcg ttagtgcagg acattggctc ggcattagcc
     2221 aacaaaccgc ctgcatcgga cgccgagatc cagttgattc gttcgcgtct ggtgcttggt
     2281 aaaacggtgg acgatctaga cctcgatatt gcggtgagca aaaacacgtt cccgattttc
     2341 ggtgcgggct gggatcgttt gatgggacgt cagaacgaga cggtgaaagt gaccaccttt
     2401 aaccgcccga aagagatggc ggaccaggtg tttacgctta atgtgctgga caataaaaac
     2461 tacaccctga gcagtgatgg cggctttagc gcccgtgggc aagcgggcca gatgctgaaa
     2521 aaagaaggcg tcacgctgat ggttgaagcc attcacgccc gcccgggcag tgagtttacc
     2581 gtcaccaaat actccacgct ggggatgatc aatcaactgc aaaacagcct gacggtaacg
     2641 gagaacggca aagacgcagg cgtactgagc ctgacttata ccggtgaaga tcgtgaacag
     2701 atccgcgaca ttcttaacag catcgcccgt aactatcagg aacaaaatat tgagcgcaaa
     2761 tcggcggaag cgtcgaaaag cctcgctttc ctcgcgcaac agttaccgga agtacgtagc
     2821 cgcctcgatg ttgccgaaaa caaactgaat gccttccgtc aggataaaga ttctgttgat
     2881 ctgccgctgg aagcgaaagc ggtgctcgat tcgatggtga acatcgacgc gcagttgaac
     2941 gaactgacct ttaaagaggc ggaaatctcc aagctgtaca ccaaagttca ccccgcgtac
     3001 cgcacgctgc tggagaaacg tcaggcgctg gaagacgaaa aagccaaact taatggtcgc
     3061 gtaacggcga tgccgaaaac ccagcaggaa attgtccgtc tgacccgcga tgtcgaatct
     3121 ggtcagcagg tctatatgca actgctgaat aaagagcagg agctgaaaat caccgaagcc
     3181 agcaccgtcg gcgatgtgcg cattgttgac ccggcaatca ctcagcctgg tgtgctaaaa
     3241 ccgaagaaag ggctgattat cctcggcgcg attatccttg gcctgatgct ctctatcgtg
     3301 ggtgtgctgc tgcgctcgtt gtttaatcgc ggcatcgaaa gcccgcaggt gctggaagaa
     3361 cacggtatca gcgtctatgc cagcatcccg ttgtcggaat ggcagaaagc gcgcgatagc
     3421 gtcaaaacca tcaaagggat taaacgctat aaacagagcc agctactggc ggtggggaat
     3481 ccaaccgatc tggcgattga agccatccgc agtcttcgta ccagtttgca cttcgcgatg
     3541 atgcaggcgc agaacaatgt gttgatgatg accggggtta gcccgtcaat cggtaaaacc
     3601 tttgtctgcg ccaacctggc ggcggtgatc agccagacca ataaacgcgt gttgttgatc
     3661 gactgcgata tgcgcaaagg ctacacccac gagctgttgg gcactaataa cgttaatggc
     3721 ctgtcggaaa ttctgattgg tcagggcgat attactaccg ctgctaaacc gacctctatt
     3781 gccaaatttg acctgatccc gcgcggtcag gtgccgccaa atccttctga actgttgatg
     3841 agcgaacgct ttgccgaact ggtgaactgg gcgagtaaaa actatgacct ggtgttgatt
     3901 gatacgccgc cgattctggc agtgaccgat gcggcaattg ttggtcgtca tgttggaacc
     3961 acgttaatgg tggcgcgtta tgcggtcaac acattgaaag aagtggaaac cagtctgagc
     4021 cgctttgagc aaaatggtat tccggtgaaa ggggtgattc tgaactccat cttccgccgc
     4081 gccagcgcgt atcaggatta tggctattac gaatacgaat ataagtcgga tgcgaaataa
     4141 cgacgcctgc attcgcaccg ccccgtaggc cggataaggc gctcacgccg catccggcaa
     4201 gcaaaccagc tcataagccg ggagaacaac ctatgaaaga caatccgctg atctcaatct
     4261 atatgccaac ctggaaccgc caacaactgg cgattcgggc aataaaatcg gttttgcgcc
     4321 aggactacag caactgggag atgatcatcg tggatgattg ctccacctcc tgggaacaac
     4381 tgcaacagta cgtcaccgcc ctcaacgatc cgcgtatcac ttacattcac aacgatatta
     4441 acagcggggc gtgcgcggta cgtaaccagg cgattatgct ggcgcaaggg gaatatatca
     4501 ccgggattga tgacgatgac gaatggacac ccaaccgtct gagcgtcttc ctcgcccata
     4561 aacagcaact ggtcacacac gcctttttgt acgctaacga ctacgtatgc cagggagaag
     4621 tttattccca accggcaagc ttaccactgt atcccaaatc gccgtactca cgccgcctgt
     4681 tctataaacg caatatcatt ggcaatcagg tctttacctg ggcatggcgt ttcaaagagt
     4741 gtttgttcga taccgaactg aaagcagcac aggattacga catcttcctg cggatggtgg
     4801 tggagtacgg cgaaccgtgg aaagtggaag aggcgacgca gatcctgcat atcaatcacg
     4861 gtgaaatgca gatcacctcg tcaccgaaaa aattctccgg gtacttccac ttttaccgca
     4921 agcacaaaga taaatttgac cgtgcaagca aaaaatatca gctgtttacc ctctaccaga
     4981 tccgcaataa acgtatgacc tggcgcactt tgctgacgct gctgtcggtg cgtaacggca
     5041 aacgtctggc tgatggtatt cgggggcgct aaagatgctg gaagatctgc gtgccaacag
     5101 ctggagttta cgcccgtgct gcatggttct tgcctatcgt gttgctcatt tttgttcggt
     5161 gtggcgcaaa aagaacgtcc tcaacaatct gtgggcggcc ccgctgctgg tgctgtatcg
     5221 cattatcacc gaatgctttt tcggttatga aatccaggcc gccgcgacca ttggccgccg
     5281 ctttactatc catcacggtt acgccgtagt gatcaataaa aacgtggtag cgggggatga
     5341 tttcaccatt cgccacggcg tcactatcgg caatcgtggt gccgataaca tggcatgtcc
     5401 acacattggc aacggcgtcg aactcggtgc caacgtcatt attcttggtg atatcacgct
     5461 tggtaacaac gtcaccgtgg gcgcgggcag cgtggtgctc gactctgtcc cggacaacgc
     5521 gctggtggtg ggagaaaaag cgcgagtgaa ggtaattaaa tgaatattct gcaatttaat
     5581 gtgcgactgg cggaaggcgg ggcggcaggt gtggcgttag atctccatca gcgtgcgctg
     5641 caacaggggc tggcgtcaca ttttgtctac ggctacggca aaggcggcaa agagagcgtc
     5701 agccatcaaa actatccgca ggtcgtcaaa catacgccgc ggatgaccgc gatggcgaac
     5761 attgcgttgt ttcgtctgtt taatcgcgat ctgtttggca atttcaatga gttatatcgc
     5821 accattactt gcacaccggg tccggtggtc ctgcattttc atgtgctgca cagctactgg
     5881 ctaaatctta agagcgtggt gcgcttttgc gaaaaggtga aaaaccacaa accggatgtc
     5941 actctggtct ggacgctgca cgaccactgg agcgttaccg ggcgctgcgc ctttactgac
     6001 ggttgtgaag gctggaaaac gggctgccag aaatgcccga ccttaaataa ttatccgccg
     6061 gtgaagattg atcgggcgca ccagctggtg gcgggcaaac gccagttatt ccgtgagatg
     6121 ctggcgctgg gctgtcagtt tatttccccc agccagcatg tggctgacgc tttcaatagc
     6181 ctgtacggtc cagggcgttg ccggattatc aataacggta ttgatatggc aaccgaagcg
     6241 attctggcgg atctgcctcc ggtgcgcgaa acccagggta agccgaaaat cgcggtggtg
     6301 gcgcatgacc tgcgttacga cggcaaaact aaccagcaac tggtgcgtga gatgatggcg
     6361 ctgggcgaca aaatcgaact gcataccttt ggtaagttct cgccgttcac cgctggcaac
     6421 gtggttaatc acggctttga aactgacaag cgcaagttga tgagcgcgct caatcagatg
     6481 gatgcgctgg tgttcagttc tcgcgtcgat aactacccgc tgattttgtg tgaggcgcta
     6541 tcgattggcg tgccggtgat tgccacccat agcgacgcgg cgcgggaagt gctgcaaaaa
     6601 tccggcggta aaaccgtcag cgaagaagac gtgctgcaac tggtgcagtt aagcaaaccg
     6661 gaaatcgcgc aggcgatatt tggtaccacg ctggctgagt tcagccaacg cagccgcgcg
     6721 gcctacagtg gacaacagat gctggaggag tatgtcaact tctatcagaa tctgtagcta
     6781 cctgttgttg ccgctgattt atctgctggt taacgtcaaa atcgcccagc ttggcgaaag
     6841 tttccccatc accatcgtca cttttttacc tgtcttgtta ctgctgtttt tagagcgcat
     6901 cagcgttaaa aaattgatga ttgccttagg gattggtgcg ggactcacgg cgtttaacta
     6961 tctgtttggt cagtcgctgg atgccagtaa atacgtcact tcaactatgc tgtttgtcta
     7021 tattgtgatc attattggca tggtgtggag tattcgtttt aaaacaattt cgccacacaa
     7081 ccatcgcaag atattacgtt tcttttatct ggtggtcggg ttggtggtgg cgctggcggc
     7141 ggtggagatg gcgcaaatta tcctcaccgg cggcagcagt attatggagt cgatttcgaa
     7201 atatctgatt tacagcaaca gctatgtact gaatttcatt aaattcggcg gcaagcgcac
     7261 gacagcactt tatttcgaac cggcattttt cgctctggca ttaatctcaa tttggctcag
     7321 catcaaacag tttggtatca aaacgcctaa aaccgatgct atgattctcg cagggataat
     7381 attatccgga tcgttttcag gggttatgac ctttatcctg ttttatttgc tggagtgggc
     7441 atttcaatat ctgaataaag aggcgattaa gaaaaagtta ccgttagcat tgatttctct
     7501 ggctgtattc ctggttggtg tggtaatcgc gtttccttat atttccaccc gtctgggcga
     7561 tttaggtacg gaaggatcgt catcgtatta tcgtattgtc ggtccgctgg tgatggtcgg
     7621 ttattctttg acccatattg acggtgtagt cagatttggc tcactttatg aatatgtcgc
     7681 atcattcgga atatttaacg gtgcggatgt cggaaaaacc atagacaatg gtttgtatct
     7741 gctgattatt tatttttcct ggttcgcagt gtttttatcg ctgtggtaca tggggaaagt
     7801 gataaaaatg atgatcaacg cttttggtga taaccgcaat tttcgcgtgc aattatatct
     7861 ttttactccg gtgtcgctgt tttttaccgg ttcgatattt agcccggaat atgcattttt
     7921 aatcgtctgt ccgtttattt tgcgaaaagc gttaaatatt acgaggtaag aataagaaca
     7981 tgttgcttag cataatcact gtcgcgtttc gtaacctcga agggatagtc aaaacacatg
     8041 cctcgctggc gcatctggcg caggcggaag atatcagctt cgaatggatt gttgtcgatg
     8101 gcggttccaa tgacggcacc cgtgagtatc tggaaaatct caatggtatc tataacctac
     8161 gctttgtcag cgagccagat aacggtatct acgacgccat gaacaaaggc attacgatgg
     8221 cacaaggcaa gttcgcgttg tttctcaatt cgggcgatat ttttcatcag gatgccgcaa
     8281 attttgtccg taagttaaaa atgcaaaaag ataacatgat gatcaccggc gatgcgctgc
     8341 tggattttgg cgacgggcat aaaattaaac gtagcgccaa accgggctgg tatatttatc
     8401 acagcctgcc cgccagtcat caggcgatat ttttccctgt atccggcttg aaaaaatggc
     8461 gttatgacct ggaatataaa gtttcttccg actacgcgct ggcagccaaa atgtataaag
     8521 ccggttatgc atttaaaaaa ctcaatggcc tggtgtctga attttccatg ggtggggtat
     8581 ctaccaccaa taatatggaa ttgtgtgctg acgcgaaaaa agtccaacga caaatattac
     8641 atgtacctgg cttttgggct gaattatcct ggcatttacg ccaacgtact acctcaaaga
     8701 cgaaagcctt atataacaaa gtctgaatat aaggagaacc aatgcaagat ttaagcggtt
     8761 tctcggtgcc gaaagggttc cggggcggca acgctattaa agtgcaatta tggtgggcag
     8821 tacaggcaac aatatttgcc tggtcgccac aagtattgta tcgctggcgg gcttttttat
     8881 tacgtttatt cggagcaaaa ataggaaaaa acgtagttat tcgtccgtca gtaaaaatta
     8941 cctatccgtg gaaattaacc ttaggtgatt acgcgtgggt cggcgatgac gtcaatttat
     9001 ataccctcgg tgaaataacc attggcgcac attcggtgat atcgcaaaaa agttatttat
     9061 gcaccggtag ccacgaccat gcaagtcaac atttcaccat taacgccacg cctattgtga
     9121 ttggcgagaa atgctggctg gcaaccgatg tctttgttgc cccaggcgtc acaatcggcg
     9181 acggcaccgt cgtgggtgca cgaagcagtg tttttaaatc gcttccggca aatgtggttt
     9241 gccgggggaa tcccgcagtg gtgatacgcg aacgcgttga aactgaataa attcaaaaat
     9301 acagaggaat aagacatgtc aaaagtcgct ctcatcaccg gtgtaaccgg acaagacggt
     9361 tcttacctgg cagagtttct gctggaaaaa ggttacgagg tgcatggtat taagcgtcgc
     9421 gcatcgtcat tcaacaccga gcgcgtggat cacatttatc aggatccgca cacctgcaac
     9481 ccgaaattcc atctgcatta tggcgacctg agtgatacct ctaacctgac gcgcattttg
     9541 cgtgaagtac agccggatga agtgtacaac ctgggcgcaa tgagccacgt tgcggtctct
     9601 tttgagtcac cagaatatac cgctgacgtc gacgcgatgg gtacgctgcg cctgctggag
     9661 gcgatccgct tcctcggtct ggaaaagaaa actcgtttct atcaggcttc cacctctgaa
     9721 ctgtacggtc tggtgcagga aattccgcag aaagaaacca cgccgttcta cccgcgatct
     9781 ccgtatgcgg tcgccaaact gtacgcctac tggatcaccg ttaactaccg cgaatcctac
     9841 ggcatgtacg cctgtaacgg tattctcttc aaccatgaat ccccgcgccg cggcgaaacc
     9901 ttcgttaccc gcaaaatcac ccgcgcaatc gccaacatcg cccaggggct ggagtcgtgc
     9961 ctgtacctcg gcaatatgga ttccctgcgt gactggggcc acgccaaaga ctacgtaaaa
    10021 atgcagtgga tgatgctgca acaggaacag ccagaagatt tcgttatcgc gaccggcgtt
    10081 cagtactccg tacgtcagtt cgtggaaatg gcggcagcac agctgggcat caaactgcgc
    10141 tttgaaggca cgggcgttga agagaagggc attgtggttt ccgtcaccgg gcatgacgcg
    10201 ccgggcgtta aaccgggcga tgtgattatc gccgttgacc cgcgttactt ccgtccggcg
    10261 gaagttgaaa cgctgctcgg cgacccgacc aaagcgcacg aaaaactggg ctggaaaccg
    10321 gaaatcaccc tcagagagat ggtgtctgaa atggtggcta atgacctcga agcggcgaaa
    10381 aaacactctc tgctgaaatc tcacggctac gacgtggcga tcgcgctgga gtcataagca
    10441 tgagtaaaca acgaattttt atcgctggcc atcgcgggat ggtcggttcc gccatcaagc
    10501 gacagctcga acagcgcggt gatgtggaac tggtattacg cacccgcgac gagctgaacc
    10561 tgctggacag ccgcgccgtg catgatttct ttgccagcga acgtattgac caggtctatc
    10621 tggcggcggc gaaagtgggc ggcattgttg ccaacaacac ctatccggcg gatttcatct
    10681 accagaacat gatgattgag agcaacatca ttcacgccgc gcatcagaac gacgtgaaca
    10741 aactgctgtt tctcggatcg tcctgcatct acccgaaact ggcaaaacag ccgatggcag
    10801 aaagcgagtt gttgcagggc acgctggagc cgactaacga gccttatgct attgccaaaa
    10861 tcgccgggat caaattgtgc gaatcttaca atcgccagta cggacgagat taccgttcag
    10921 tcatgccgac caacctgtac gggccacacg acaacttcca cccgagtaat tcgcatgtga
    10981 tccctgcatt gctgcgtcgc ttccacgagg cgacggcaca gaatgcgccg gacgtggtgg
    11041 tgtggggcag cggtacaccg atgcgtgaat tcctgcacgt cgatgatatg gcggcggcaa
    11101 gcattcatgt gatggagctg gcgcacgaag tctggctgga gaacacccag ccgatgctgt
    11161 cgcacattaa cgtcggcacg ggcgttgact gcactatccg cgagctggcg caaaccatcg
    11221 ccaaagtggt gggttacaaa ggtcgggtgg tttttgatgc cagcaaaccg gatggcacac
    11281 cgcgcaaact gctggatgtg atgcgcctgc atcagcttgg ctggtatcac gaaatatcac
    11341 tggaagcggg gcttgccagc acttaccagt ggttccttga gaatcaagac cgctttcggg
    11401 ggtaatgatg tttttacgtc aggaagactt tgccacggta gtgcgctcca caccgcttgt
    11461 ctctctcgac tttattgtcg agaacagtcg cggcgagttt ctgcttggca aaagaaccaa
    11521 ccgcccggcg cagggttact ggtttgtgcc gggagggcgc gtgcagaaag acgaaacgct
    11581 ggaagccgca tttgagcggc tgacgatggc ggaactgggg ctgcgtctgc cgataacagc
    11641 aggccagttt tacggtgtct ggcagcactt ttatgacgat aacttctccg gcacggattt
    11701 caccactcac tatgtggtgc tcggttttcg cttcagagtc gctgaagaag atctgttact
    11761 gccggatgag cagcatgacg attaccgctg gctgacgccg gacgcgctgc tcgccagtga
    11821 caatgttcac gctaacagcc gcgcctattt tctcgctgag aagcgtgccg gagtacccgg
    11881 attatgaaaa tactggtcta cggcattaac tactcgccgg agttaaccgg catcggcaaa
    11941 tacaccggcg agatggtgga atggctggcg gcacaaggtc atgaggtgcg ggtcattacc
    12001 gcaccgcctt actacccgca gtggcaggtg ggcgagaact attccgcctg gcgctacaaa
    12061 cgagaagagg gggccgccac ggtgtggcgc tgcccgctgt atgtgccaaa acagccgagc
    12121 accctgaaac gcctgttgca tctcggcagt tttgccgtca gcagtttttt cccgctgatg
    12181 gcgcaacgtc gctggaagcc ggagcgcatt atcggcgtag tgccaacgct gttttgcacg
    12241 ccgggaatgc gtctgctggc gaaactctct ggcgcgcgta ccgtgctgca tattcaggat
    12301 tacgaagtag atgccatgct ggggctgggc cttgccggaa aaggcaaagg cggcaaagtg
    12361 gcacagctgg caacggcgtt cgaacgtagc ggactacata acgtcgataa cgtctccacg
    12421 atttcgcgtt cgatgatgaa taaagccatc gaaaaaggcg tggcggcgga aaacgtcatc
    12481 ttcttcccca actggtcgga aatcgcccgt tttcagcatg ttgcagatgc cgatgttgat
    12541 gcccttcgta accagcttgg cctgccggat aacaaaaaaa tcattcttta ctccggcaat
    12601 attggtgaaa agcaggggct ggaaaacgtt attgaagctg ccgatcgcct gcgcgatgaa
    12661 ccactgattt ttgccattgt cgggcagggt ggcggcaaag cgcgactgga aaaaatggcg
    12721 cagcagcgtg gactgcacaa catgcaattt ttcccgctgc aatcgtatga cgctttaccc
    12781 gcactgctga agatgggcga ttgccatctg gtggtgcaaa aacgcggcgc ggcagatgcc
    12841 gtattgccgt cgaaactgac caatattctg gcggtaggcg gtaacgcggt gattaccgca
    12901 gaggcccaca cagaactggg ggagctttgc gaaacctttt cgggcattgc ggtttgcgta
    12961 gaaccggaat cggtcgaggc gctggtggcg gggatccgtc aggcgctcct gctgcccaaa
    13021 cacaacacgg tggcacgtga atatgccgaa cgcacgctcg ataaagagaa cgtgttacgt
    13081 caatttataa atgatattcg gggataatta tggcgcagtc gaaactctat ccagttgtga
    13141 tggcaggtgg ctccggtagc cgcttatggc cgctttcccg cgtactttac cccaagcagt
    13201 ttttatgcct gaaaggcgat ctcaccatgc tgcaaaccac catctgccgc ctgaacggcg
    13261 tggagtgcga aagcccggtg gtgatttgca atgagcagca ccgctttatt gtcgcggaac
    13321 agctgcgtca actgaacaaa ctcaccgaga acattattct cgaaccgaca gggcgtaaca
    13381 ctgcacctgc cattgcactg gcggcgctgg cggcaaaacg tcatagcccg gagagcgacc
    13441 cgttaatgct ggtattggcg gcggatcatg tgattgccga tgaagacgcg ttccgtgccg
    13501 ccgtgcgtaa tgccatgcca tatgccgaag cgggcaagct ggtgaccttc ggcattgtgc
    13561 cggatctacc tgaaaccggt tatggctata ttcgtcgcgg tgaagtgtct gcgggtgagc
    13621 aggatacggt ggcctttgaa gtggcgcagt ttgtcgaaaa accgaatctg gaaaccgccc
    13681 aggcctatgt ggcaagcggc gaatattact ggaacagcgg catgttcctg ttccgcgccg
    13741 gacgctatct cgaagaactg aaaaaatatc gcccggatat cctcgatgcc tgcgaaaaag
    13801 cgatgagcgc cgtcgatccg gatctcgatt ttattcgcgt ggatgaagaa gcgtttctcg
    13861 cctgcccgga agagtcggtg gattacgcgg tcatggaacg tacggcagat gccgttgtgg
    13921 tgccgatgga tgcgggctgg agcgatgtcg gttcctggtc ttcattatgg gagatcagcg
    13981 cccacaccgc cgagggcaac gtttgccacg gcgatgtgat taatcacaaa acagaaaaca
    14041 gctatgtgta tgccgaatct ggcctcgtaa ccaccgtcgg ggtgaaagat ctggtggtag
    14101 tgcagaccaa agatgcggtg ctgattgccg accgtaacgc ggtgcaggat gtgaaaaaag
    14161 tggtcgagca gatcaaagcc gatggtcgcc atgagcatcg ggtacatcgc gaagtgtatc
    14221 gtccgtgggg caaatatgac tctatcgacg cgggcgaccg ctaccaggtg aaacgcatca
    14281 ccgtgaaacc gggcgaaggc ttgtcggtac agatgcacca tcaccgcgcg gaacactggg
    14341 tggtggtcgc gggaacggca aaagtcacta ttgacggtga tatcaaactg cttggtgaaa
    14401 acgagtccat ttatattccg ctgggggcga cgcactgcct ggaaaacccg gggaaaattc
    14461 cgctcgattt aattgaagtg cgctccggct cttatctcga agaggatgat gtggtgcgct
    14521 tcgcggatcg ctacggacgg gtgtaaacgt cgcatcaggc gctgtttgtc ggatgcggcg
    14581 tgaacgcctt atccgaccta cggttcggat ttgtaggcct gataagacgc ggcagcgtcg
    14641 catcaggcaa tgaatgcgaa accgcgatgt aaataacgac aaaaacaaaa ttggccgttt
    14701 cggtcagggc caactattgc ctgaaaaagg gtaacgatat gaaaaaatta acctgcttta
    14761 aagcctatga tattcgcggg aaattaggcg aagaactgaa tgaagatatt gcctggcgca
    14821 ttggtcgcgc ctatggcgaa tttctcaaac cgaaaaccat tgtgttaggc ggtgatgtcc
    14881 gcctcaccag cgaaacctta aaactggcgc tggcgaaagg tttacaggat gcgggcgtcg
    14941 atgtgctgga tattggcatg tccggcaccg aagagatcta tttcgccacg ttccatctcg
    15001 gcgtggatgg cggcattgaa gttaccgcca gccataatcc gatggattat aacggcatga
    15061 agctggttcg cgagggggct cgcccgatca gcggagatac cggactgcgc gacgtccagc
    15121 gtctggctga agccaacgac ttccctcccg tcgatgaaac caaacgcggt cgctatcagc
    15181 aaatcaacct gcgtgacgct tacgttgatc acctgttcgg ttatatcaac gtcaaaaacc
    15241 tcacgccgct caagctggtg atcaactccg ggaacggcgc agcgggtccg gtggtggacg
    15301 ccattgaagc ccgctttaaa gccctcggcg cacccgtgga attaatcaaa gtgcacaaca
    15361 cgccggacgg caatttcccc aacggtattc ctaacccact actgccggaa tgccgcgacg
    15421 acacccgcaa tgcggtcatc aaacacggcg cggatatggg cattgccttt gatggcgatt
    15481 ttgaccgctg cttcctgttt gacgaaaaag ggcagtttat tgagggttac tacattgtcg
    15541 gcctgctggc agaagcgttc ctcgaaaaaa atcccggcgc gaagatcatc cacgatcctc
    15601 gtctctcctg gaataccgtt gatgtggtga ctgccgcagg cggcaccccg gtaatgtcga
    15661 aaaccggaca cgcctttatt aaagaacgta tgcgcaagga agacgccatc tacggtggcg
    15721 aaatgagcgc ccaccattac ttccgtgatt tcgcttactg cgacagcggc atgatcccat
    15781 ggctgctggt cgccgaactg gtgtgcctga aaggaaaaac gctgggcgaa ctggtgcgcg
    15841 accggatggc ggcgtttccg gcaagcggtg agatcaacag caaactggcg caacccgttg
    15901 aggcgattaa ccgcgtggaa cagcacttta gccgtgaggc gctggcggtg gatcgcaccg
    15961 atggcatcag catgaccttt gccgactggc gctttaatct gcgctcctcc aacaccgaac
    16021 cggtggtgcg gttgaatgtg gaatcgcgcg gtgatgtacc gctgatggaa gcgcgaacgc
    16081 gaactctgct gacgttgctg aacgagtaat gtcattactt tccccctcac cctgacgctc
    16141 tactgcctgc ggagagaagt gagcacggaa ctttcttctc cccctcgccc ctttggggag
    16201 agggccgggg tgaggggcga ggcgacaata gaacaggaac aacgatgaca aatctaaaaa
    16261 agcgcgagcg agcgaaaacc aatgcatcgt taatctctat ggtgcaacgc ttttcagata
    16321 tcaccatcat gtttgccgga ctatggctgg tttgcgaagt gagcggactg tcattcctct
    16381 acatgcacct gttggtggcg ttgattacgc tggtggtgtt ccagatgctg ggcggcatca
    16441 ccgattttta tcgctcatgg cgcggtgttc gggcagcgac agaatttgcc ctgttgctac
    16501 aaaactggac cttaagcgtg attttcagcg ccggactggt ggcgttcaat aatgatttcg
    16561 acacgcaact gaaaatctgg ctggcgtggt atgggctgac cagcatcgga ctggtggttt
    16621 gccgttcatg tattcgcatt ggcgcgggct ggctgcgtaa tcatggctat aacaagcgta
    16681 tggtcgccgt agcgggggat ttagccgccg gacaaatgct gatggagagc ttccgtaatc
    16741 agccgtggtt agggtttgaa gtggtgggcg tttaccacga cccgaaaccg ggtggcgttt
    16801 ctaacgactg ggcgggcaat ctgcaacagc tggtcgagga tgcgaaagcg ggcaagattc
    16861 ataacgtcta tatcgcgatg caaatgtgcg acggcgcgcg agtgaaaaaa ctggtccatc
    16921 aactggcgga cactacctgt tcggtgctgc tgatccccga tgtctttacc ttcaacattc
    16981 tccattcccg tattgaagag atgaacggcg taccagtggt gccgctgtat gacacgccgc
    17041 tttccggggt taaccgcctg ctcaaacgtg cagaagacat tgtgctggca acgctgattc
    17101 tgctgctgat ctccccggtg ctgtgctgca ttgcgttggc ggtgaaactg agctcaccgg
    17161 ggccggttat tttccgccag actcgctacg gcatggacgg caagccgatc aaagtgtgga
    17221 agttccgttc gatgaaagtg atggagaacg acaaagtggt gactcaggcg acgcagaacg
    17281 atccgcgcgt caccaaagtg gggaactttc tgcgccgcac ctcgctggac gaattgccgc
    17341 agtttatcaa tgtgctgacc ggggggatgt cgattgtcgg tccacgtccg cacgcagtag
    17401 cgcataacga acagtatcgc cagctcattg aaggctacat gctgcgccac aaagtgaaac
    17461 cgggcattac cggctgggcg cagattaatg gctggcgcgg cgaaaccgac acgctggaga
    17521 aaatggaaaa acgcgtcgag ttcgaccttg agtacatccg cgaatggagc gtctggttcg
    17581 atatcaaaat cgttttcctg acggtattca aaggcttcgt taacaaagcg gcatattgat
    17641 atgagcttac gtgaaaaaac catcagcggc gcgaagtggt cggcgattgc cacggtgatc
    17701 atcatcggcc tcgggctggt gcaaatgact gtgctggcgc ggattatcga caaccaccag
    17761 ttcggtttgc tgactgtgtc gctggtgatt atcgcgctgg cagatacgct ttctgacttc
    17821 ggtatcgcta actccattat tcagcgaaaa gaaatcagcc accttgaact caccacgttg
    17881 tactggctga acgtcgggct ggggatcgtg gcgtgtgtgg cggtattttt gttgagtgat
    17941 gccattggcg acatgctgaa taacccggac ctggcaccgt tgattaaaac attgtcacta
    18001 gcgtttgtgg tgatccccca cgggcaacag ttccgcgcgt tgatgcaaaa agagctggag
    18061 ttcaacaaaa tcggcatgat cgaaaccagc gcggtgctgg cgggctttac ttttacggtg
    18121 gttagcgccc atttctggcc gctggcgatg accgcgatcc tcggttatct ggtcaatagt
    18181 gcggtgagaa cgctgctgtt tggctacttt ggccgcaaaa tctatcgccc cgctctgcat
    18241 ttctcgctgg cgtcggtggc accgaactta cgctttggtg cctggctgac ggcggacagc
    18301 atcatcaact atctcaacac caacctttca acactcgtgc tggcgcgtat tctcggcgcg
    18361 ggcgtggctg ggggatacaa cctggcgtac aacgtggccg ttgtgccgcc gatgaaactg
    18421 aacccgatca tcacccgcgt gttgtttccg gcgtttgcca aaattcagga cgataccgaa
    18481 aagctgcgcg tcaacttcta caagctgctg tcggtagtgg ggattatcaa ctttccggcg
    18541 ctgctggggc tgatggtggt gtcgaataac tttgtaccgc tggtatttgg cgaaaagtgg
    18601 agcagcatta ttccggtgct acagctgctg tgtgtggtgg gtctgctgcg ctccgtgggt
    18661 aacccgattg gttcgctgct gatggcgaaa gcgcgggtcg atatcagctt taaattcaac
    18721 gtatttaaaa catttctgtt tattccggcg attgttattg gtgggcagat ggcgggcgca
    18781 atcggcgtca cgcttggctt cctgctggtg cagattatca acactattct gagctatttc
    18841 gtgatgatta aaccggttct tggttccagt tatcgccagt acatcctgag tttgtggctg
    18901 ccgttttatc tctcgctgcc aacgctggtg gtcagttatg cgctgggcat tgtgctgaaa
    18961 gggcaactgg cgctggggat gctgctggcg gtgcaaatag ccgcgggtgt gctggcgttt
    19021 gtggtgatga ttgtgctgtc gcgccatccg ctggtggtgg aagtgaagcg tcagttttgt
    19081 cgcagcgaaa aaatgaaaat gcttttacgg gcggggtgag ttgttgtttt cggttccgac
    19141 aacggttgcc gggtgcagcg taaaacgcct tatccgtcct acggttctgt gctcgcatca
    19201 ggcaaaaaac ggcaacgaac acaacaaaca cagcctcacc tggcatcaaa tttatgagga
    19261 aaaaatgaaa ttacttattc tgggcaacca cacttgcggc aatcgtggcg acagcgccat
    19321 cttgcgcggc ttacttgatg ccatcaacat cctcaatcca catgccgaag tggacgtgat
    19381 gagccgctat cctgtcagtt cttcctggct actcaaccgc ccggtaatgg gcgatccgct
    19441 gttcctgcaa atgaaacaac acaacagcgc ggcgggcgtt gtcgggcgcg ttaaaaaagt
    19501 cctccgtcgc cgctaccagc atcaggtatt gctctcacgc gtcaccgaca gtggcaagct
    19561 gcgtaatatc gccatcgccc agggattcac cgacttcgtg cgcctgctgt caggttacga
    19621 cgccattatt caggtcggcg gatcgttttt tgtcgatctc tacggcgtgc cacagtttga
    19681 acatgcactt tgcacgttta tggcgaaaaa gtcgctgttt atgattggtc acagtgttgg
    19741 cccgttccag gatgagcaat ttaaccaact ggcgaactac gtttttggtc actgcgacgc
    19801 gctgatcctg cgcgaatcgg tcagccttga actgatgaaa cgcagcaata tcaccacttc
    19861 aaaagttgaa catggcgtcg ataccgcgtg gctggtcgat caccacacag aagacttcac
    19921 cgccagctat gctgttcaac actggctgga cgttgccgca caacagaaaa cggtggcaat
    19981 taccctgcgc gaactggcac cgttcgacaa acgtctcggc accactcaac aagcatatga
    20041 aaaagccttt gccggggtgg tcaatcgcat tctcgacgaa ggctatcagg tgattgcgct
    20101 ctccacctgt acgggtatcg acagctataa caaagacgac cgcatggtgg cgctcaacct
    20161 gcgccagcac atcagcgatc ctgcccgtta ccacgtagtg atggatgaac tcaacgatct
    20221 ggaaatgggc aaaattctgg gggcctgtga actcaccatc ggtacgcgcc tgcactccgc
    20281 cattatctca atgaactttg ccaccccggc gattgccatc aattatgaac ataaatccgc
    20341 cgggattatg cagcagctgg gactaccgga gatggcgatt gatatccgtc atttattaga
    20401 cggcagcctg caagtgatgg ttgcggatac cttaggccag cttccggcac tgaacacgcg
    20461 acttaacgaa gccgtcagtc gcgaacgtca gacgggaatg cagatggtga aatctgtgct
    20521 tgaacacatc agggaggcga aatgaaggtc ggcttctttt tactgaaatt tccgctgtcg
    20581 tcggaaacct tcgtcctcaa tcaaattacc gcgtttattg atatgggctt tgaggtggag
    20641 attgtcgcgc tgcaaaaagg cgacacccag aacacccacg cggcatggac gaaatacaac
    20701 cttgccgcca gaacccgttg gttacaggac gaacctacgg gcaaagtggc gaaactgcgc
    20761 caccgtgcca gccagatctt acgcggcatt catcgtaaaa atacctggca ggcacttaac
    20821 ctcaaacgct atggtgccga gtcgcgaaac ctgattttgt ctgccatttg cggccaggtc
    20881 gcaacaccat tttatgccga tgtctttatc gctcattttg gccctgcggg ggtaaccgca
    20941 gcaaaactcc gcgaactggg tgtcattcgc ggcaaaattg ccactatctt ccacggcatt
    21001 gatatctcca gtcgggaggt gctcaaccat tacactcccg aatatcagca actgttccgc
    21061 cgtggcgatc tgatgctacc aataagcgat ctgtgggccg gaaggttgca aaaaatgggc
    21121 tgcccgaggg aaaaaatcgc cgtatcgcgc atgggcgtgg acatgacgcg ttttagcccg
    21181 cgtccggtga aagcgcccgc aacgccgctg gaaatcattt ccgtcgcacg cttaaccgag
    21241 aaaaaaggcc tgcatgtggc gattgaagcc tgccgtcagt tgaaagagca gggcgtggca
    21301 tttcgctatc gcatcctcgg cattggcccg tgggaacgac gcctgcgcac cctcatcgaa
    21361 caatatcaac tggaagatgt ggtagagatg ccgggcttta aaccgagcca cgaagtgaaa
    21421 gcgatgctcg acgacgcgga tgtcttcctg ttgccatcgg ttacaggtgc ggatggcgat
    21481 atggaaggta ttccggtggc actaatggaa gcgatggcgg tcggcattcc ggtggtttct
    21541 actctgcata gcggaatacc ggaactggtg gaggccgata aatccggctg gctggtgcct
    21601 gagaacgatg ctcgcgcact ggcgcaacga ctggcggcgt ttagccaact ggacaccgac
    21661 gaactggctc cggtcgtcaa acgtgcgcgc gaaaaagtcg aacacgattt taaccagcag
    21721 gtgatcaatc gagaactcgc cagcttgctg caggctttat agaggagaac gcatgccatt
    21781 taaaaaactc tcccgacgca ccttcctgac ggcaagctcg gtgcttgcct tcctccatac
    21841 ccctttcgct cgcgcgcttc ccgcccgaca aagcgttaac attaacgact acaaccctcg
    21901 cgactggatc gcctcattta aacaagcctt cagcgaaggg caaacggtcg tcgtgcctgc
    21961 cggattggtt tgcctcaata tcaacaccgg catctttatc cctcctggca aaacgttaca
    22021 catccttgga agcctgcgcg gcaacggcag agggcgattt gtcttacagg acggcagcca
    22081 ggtgacaggg gaggagggcg gcagtatgca taacatcacc ctggatgtgc gcggctctga
    22141 ctgcaccatc aaagggctgg cgatgagcgg ctttggtccg gtaacgcaga tttatatcgg
    22201 cggcaaaaac aaacgggtca tgcgtaacct gaccatcgat aacctgaccg ttagccacgc
    22261 taattacgcc atcttacgcc agggatttca taaccagatt atcggtgcca acatcaccaa
    22321 ttgtaagttc agcgacttac agggcgacgc cattgaatgg aatgtggcga ttaacgaccg
    22381 tgatattttg atatctgacc atgtcatcga gcgcatcaac tgtactaacg gcaaaatcaa
    22441 ttggggcatc ggcataggtc ttgcgggaag cacttatgat aacaactatc cggaagacca
    22501 ggcagtgaaa aactttgtcg tggcgaacat cacgggatcg gattgtcggc agttgataca
    22561 tgttgaaaat ggtaaacatt ttgttattcg taatatcaaa gcctgcaata tcacgccgga
    22621 tttcagtaag aaagcaggta ttgataacgc cacggtcgct atttacggtt gtgacaattt
    22681 cgtgattgat aatattgaaa tgattaatag cgccgggatg ttaatcggct atggggtaat
    22741 taaaggcaaa tatctctcga taccgcagaa tttccgagtg aataatattc agctggataa
    22801 tacccacctt gcttacaaat tgcgcggcat ccaaatctcc gccgggaatg ctgtctcctt
    22861 tgtggcgctg actaacgttg agatgaagcg tgcgtcgctg gagttacaca acaagccgca
    22921 acaccttttt atgcgtaata tcaaagtgat gcaggaatcc tctgttggac ccgcattgag
    22981 catgaacttc gacatgcgca aagacgttcg cggcgtgttt atggcgaaaa aagaaacact
    23041 gctatctctt gcaaatgttc atgcggtgaa tgaaagaggg caaagctccg tcgatatcga
    23101 caggataaat caccatattg ttaatgtgga aaagattaac tttagattgc cggaacggag
    23161 ggagtagatt tgcgaccatt cctggaaaaa tggagccata cttaggaaca atgctactgc
    23221 aatctacaac gaagcggcgt aacatcacaa gtaattcagt aatcaattca gggtaattga
    23281 tgctggcgaa aaaaatcgaa caagctataa ttcagcaacc attttacagg tggatgaaat
    23341 aatgacgaat ttaaaagcag ttattcctgt agcgggtctt gggatgcata tgttgcctgc
    23401 cactaaggcg attcccaaag agatgctacc gatcgtcgac aagccaatga ttcagtacat
    23461 tgttgacgag attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa
    23521 gaacgcggtc gaaaaccact tcgacacctc ttatgaatta gaatctcttc ttgaactgcg
    23581 cgtgaagcgt caactgctgg cggaagtaca gtccatctgt ccgccaggcg tgaccattat
    23641 gaacgtgcgt cagggcgaac ctttaggttt aggccactcc attttgtgtg cacgacccgc
    23701 cattggtgac aatccatttg tcgtggtact gccggacgtt gtgatcgacg acgccagtgc
    23761 cgacccgctg cgctacaacc ttgctgccat gattgcgcgt ttcaatgaaa cgggccgtag
    23821 ccaggtgctg gcaaaacgta tgccgggtga gctctctgaa tactctgtca tccagaccaa
    23881 agaaccgctg gatcgcgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga
    23941 tcagccacaa acgctggact cagacatcat ggccgttggt cgctatgtac tttctgccga
    24001 tatttggccg gaactggaac gtactcagcc tggtgcatgg ggacgtattc aactgactga
    24061 tgctattgcc gagctggcga aaaaacagtc cgttgatgcc atgctgatga ctggggacag
    24121 ttacgactgc ggtaaaaaaa tgggctatat gcaggcgttc gtgaagtatg gattacgaaa
    24181 cctgaaagaa ggggcgaagt tccgtaaagg cattgagaag ctgttaagca aataatgaaa
    24241 atctgaccgg atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtgatgaa
    24301 agtaatttgt tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag
    24361 ttagcaatag gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg
    24421 taagacaatt agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc
    24481 gtagacatgc atgcagtgca ctggtagctg taaagccagg ggcggtagcg tgcattaata
    24541 cccctattaa tcaacctaag agccgcttat ttcacagcat gctctgaagt aatatggaat
    24601 aataaagtga agatacttgt tactggtggc gcaggattta ttggttctgc tgtagttcgt
    24661 cacattataa ataatacgca ggatagtgtt gttaatgtcg ataaattaac gtacgccgga
    24721 aacctggaat cacttgctga tgtttctgat tctgaacgct atgtttttga acatgcggat
    24781 atttgcgatg cagctgcaat ggcgcggatt tttgctcagc atcagccgga tgcagtgatg
    24841 cacctggctg ctgaaagcca tgttgaccgt tcaattaccg gccctgcggc atttattgaa
    24901 accaatattg ttggtactta tgtccttttg gaagccgctc gcaattactg gtctgctctt
    24961 gatagcgaca agaaaaatag cttccgtttt catcatattt ctactgacga agtctatggt
    25021 gatttgcctc atcctgacga agtaaataat acagaagaat tacccttatt tactgagacg
    25081 acagcttacg cgccaagcag tccttattcc gcatccaaag catctagcga tcatttagtc
    25141 cgcgcgtgga aacgtaccta tggtttaccg accattgtga ctaactgttc gaataactac
    25201 ggcccttatc actttccgga aaaattgatt ccgctggtaa ttcttaatgc tctggaaggt
    25261 aaggcattac ctatttatgg caaaggggat caaattcgcg attggctata tgttgaagat
    25321 catgcgcgtg cgttatatac cgttgtcacc gaaggtaaag cgggtgaaac ttataacatt
    25381 ggcggacaca acgaaaagaa aaacatcgat gttgtgctga ctatttgtga tttgttggat
    25441 gagattgtac cgaaagagca atcttatcgt gagcaaatta cttatgttgc cgatcgtccg
    25501 ggacacgatc gccgttatgc gattgatgct gagaagatta gccgcgaatt gggctggaaa
    25561 ccgcaggaaa cgtttgagag cgggattcgg aagacagtgg aatggtacct gtccaataca
    25621 aaatgggtcg aaaatgtgaa aagtggtgcc tatcagtcat ggattgcaca gaactatgag
    25681 ggccgtcagt aatgaatatc ctccttttcg gcaaaacagg gcaggtaggt tgggaactac
    25741 agcgtgctct ggcacctctg ggtaatttga ttgctcttga tgttcactct actgattatt
    25801 gcggtgattt tagtaatcct gaaggtgtag ctgaaaccgt aagaagcatt cggcctgata
    25861 ttattgtcaa cgcagccgct cacaccgcag tagacaaagc agaatcagaa ccggagtttg
    25921 cacaattact taacgcaaca agtgtcgaag cgattgcgaa agcagccaat gaagtcggcg
    25981 cctggcttat tcattactcg actgattacg tcttccctgg aaatggcgat atgccatggc
    26041 gggagacgga tgcaaccgca ccactaaatg tttacggtga aaccaagtta gccggagaaa
    26101 aagcgttaca ggaatattgc gcaaagcatc ttattttccg gaccagctgg gtctatgcag
    26161 gaaaaggaaa taacttcgcc aaaacgatgt tacgtctggc aaaagagcgt gaagaattag
    26221 cggttattaa tgatcagttt ggtgcgccaa caggtgctga actgctggct gattgtacag
    26281 cacatgccat tcgtgtcgca ctgaataaac cagaagtcgc aggcttgtac catctggtag
    26341 ccagtggtac cacaacctgg cacgattatg ctgcgctggt ttttgaagag gcacgcaaag
    26401 caggtattcc ccttgcactc aacaagctca acgcagtacc aacaacagcc tatcctacac
    26461 cagctcgtcg tccgcataac tctcgcctta atacagaaaa atttcagcag aaatttgcgc
    26521 ttgttttgcc tgattggcag gttggcgtga aacgaatgct caacgaatta tttacgacta
    26581 cagcaattta atagtttttg catcttgttc gtgatggtgg agcaagatga attaaaagga
    26641 atgatgaaat gaaaacgcgt aaaggtatta ttttagctgg tggttcgggt actcgtcttt
    26701 atcctgtaac tatggctgtc agtaaacagt tgttaccgat ttatgataaa ccgatgatct
    26761 attacccgtt gtctacactg atgttagcgg gtcttcgcga tattctgatt attagtacgc
    26821 cacaggatac tcctcgtttt caacaactgc tgggtgacgg gagccagtgg gggctaaatc
    26881 ttcagtacaa agtgcaaccg agtccagatg gtcttgcgca ggcatttatc atcggtgaag
    26941 agtttatcgg tggtgatgat tgtgctttgg ttctaggtga taatatcttt tacggtcacg
    27001 atctgccgaa gttaatggat gtcgctgtta acaaagaaag tggtgcaacg gtatttgcct
    27061 atcacgttaa tgatcctgaa cgctacggtg tcgttgagtt tgataaaaac ggtacggcga
    27121 tcagcctgga agaaaaaccg ctacaaccaa aaagtaatta tgcggtaacc gggctttatt
    27181 tttatgataa ctacgttgtc gaaatggcga aaaatcttaa gccttctgcc cgcggtgaac
    27241 tggaaattac cgatattaac cgtatttata tggaacaggg gcgtttatcc gttgccatga
    27301 tgggacgtgg ttatgcatgg ctggacacgg ggacacatca aagtcttatt gaggcaagca
    27361 attttatcgc aacaatagaa gaacgtcagg ggctgaaagt ttcctgcccg gaagaaattg
    27421 cttaccgtaa agggtttatc gatgctgagc aggtgaaagt attagctgaa ccgttgaaga
    27481 aaaatgctta tggtcagtat ctgctgaaaa tgattaaagg ttattaataa aatgaacgta
    27541 attaaaacag aaattccaga tgtactgatt tttgaaccga aagtttttgg tgatgagcgt
    27601 ggtttcttta tggaaagctt taatcagaaa gtttttgaag aagctgtagg gcggaaggtt
    27661 gaatttgttc aggataatca ttctaaatca tctaagggtg tgttacgcgg actgcactat
    27721 cagctggaac cctatgctca aggtaaatta gttcgttgtg tggtcggtga agtatttgat
    27781 gtagcagttg atattcgtaa atcgtcacct acatttggga aatgggttgg ggtgaatttg
    27841 tctgctgaga ataagcgtca gttgtggatc cctgaggggt ttgcgcatgg ttttttggtg
    27901 ttaagcgaga tagcagagtt tgtttataag acgacaaatt attatcatcc tgaatcagaa
    27961 ggttgcataa aatgggatga ttcttttttg atgattgatt ggccaaataa accgatagca
    28021 atctcagaaa aagataaaaa aggctcatca attttgaggc taaatgacta ttgatgttga
    28081 aaaaaaaact tcaaaaaata aaggaatatc attcagtatt ggagttggca ataattcagg
    28141 gtgcgaatgc catatttcct gtgttggtat tcccattttt tcttattacc ttaggggaaa
    28201 acatcttttc aagtattgct gttggtgaag tact
//
LOCUS       DAZFRJ010000048        28113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000048 DAZFRJ010000000
VERSION     DAZFRJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.048"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            193..744
                     /gene="maa"
                     /locus_tag="ACHKUN_001839"
     CDS             193..744
                     /gene="maa"
                     /locus_tag="ACHKUN_001839"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO6660106.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPVRIIKKL"
     gene            860..1330
                     /gene="ylaC"
                     /locus_tag="ACHKUN_001840"
     CDS             860..1330
                     /gene="ylaC"
                     /locus_tag="ACHKUN_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO6660107.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            1494..3044
                     /gene="pdeB"
                     /locus_tag="ACHKUN_001841"
     CDS             1494..3044
                     /gene="pdeB"
                     /locus_tag="ACHKUN_001841"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO6660108.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(3086..3439)
                     /gene="ybaA"
                     /locus_tag="ACHKUN_001842"
     CDS             complement(3086..3439)
                     /gene="ybaA"
                     /locus_tag="ACHKUN_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO6660109.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(3559..3655)
                     /gene="ffs"
                     /locus_tag="ACHKUN_001843"
     ncRNA           complement(3559..3655)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKUN_001843"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3817..4128
                     /locus_tag="ACHKUN_001844"
     CDS             3817..4128
                     /locus_tag="ACHKUN_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO6660110.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(4159..4731)
                     /gene="ybaY"
                     /locus_tag="ACHKUN_001845"
     CDS             complement(4159..4731)
                     /gene="ybaY"
                     /locus_tag="ACHKUN_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO6660111.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            4949..5809
                     /gene="tesB"
                     /locus_tag="ACHKUN_001846"
     CDS             4949..5809
                     /gene="tesB"
                     /locus_tag="ACHKUN_001846"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO6660112.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(5858..7144)
                     /gene="amtB"
                     /locus_tag="ACHKUN_001847"
     CDS             complement(5858..7144)
                     /gene="amtB"
                     /locus_tag="ACHKUN_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO6660113.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(7174..7512)
                     /gene="glnK"
                     /locus_tag="ACHKUN_001848"
     CDS             complement(7174..7512)
                     /gene="glnK"
                     /locus_tag="ACHKUN_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO6660114.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(7693..9474)
                     /gene="mdlB"
                     /locus_tag="ACHKUN_001849"
     CDS             complement(7693..9474)
                     /gene="mdlB"
                     /locus_tag="ACHKUN_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6660115.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(9467..11239)
                     /gene="mdlA"
                     /locus_tag="ACHKUN_001850"
     CDS             complement(9467..11239)
                     /gene="mdlA"
                     /locus_tag="ACHKUN_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6660116.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(11269..11727)
                     /gene="decR"
                     /locus_tag="ACHKUN_001851"
     CDS             complement(11269..11727)
                     /gene="decR"
                     /locus_tag="ACHKUN_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO6660117.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(11880..12698)
                     /gene="cof"
                     /locus_tag="ACHKUN_001852"
     CDS             complement(11880..12698)
                     /gene="cof"
                     /locus_tag="ACHKUN_001852"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO6660118.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            12798..14498
                     /gene="ybaE"
                     /locus_tag="ACHKUN_001853"
     CDS             12798..14498
                     /gene="ybaE"
                     /locus_tag="ACHKUN_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO6660119.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            14563..15258
                     /gene="queC"
                     /locus_tag="ACHKUN_001854"
     CDS             14563..15258
                     /gene="queC"
                     /locus_tag="ACHKUN_001854"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO6660120.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(15310..15708)
                     /gene="fadM"
                     /locus_tag="ACHKUN_001855"
     CDS             complement(15310..15708)
                     /gene="fadM"
                     /locus_tag="ACHKUN_001855"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO6660121.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(15802..16173)
                     /gene="ybaV"
                     /locus_tag="ACHKUN_001856"
     CDS             complement(15802..16173)
                     /gene="ybaV"
                     /locus_tag="ACHKUN_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO6660122.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(16324..18195)
                     /gene="ppiD"
                     /locus_tag="ACHKUN_001857"
     CDS             complement(16324..18195)
                     /gene="ppiD"
                     /locus_tag="ACHKUN_001857"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6660123.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(18387..18677)
                     /gene="hupB"
                     /locus_tag="ACHKUN_001858"
     CDS             complement(18387..18677)
                     /gene="hupB"
                     /locus_tag="ACHKUN_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO6660124.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(18868..21222)
                     /gene="lon"
                     /locus_tag="ACHKUN_001859"
     CDS             complement(18868..21222)
                     /gene="lon"
                     /locus_tag="ACHKUN_001859"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO6660125.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(21410..22684)
                     /gene="clpX"
                     /locus_tag="ACHKUN_001860"
     CDS             complement(21410..22684)
                     /gene="clpX"
                     /locus_tag="ACHKUN_001860"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO6660126.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(22810..23433)
                     /gene="clpP"
                     /locus_tag="ACHKUN_001861"
     CDS             complement(22810..23433)
                     /gene="clpP"
                     /locus_tag="ACHKUN_001861"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO6660127.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(23680..24978)
                     /gene="tig"
                     /locus_tag="ACHKUN_001862"
     CDS             complement(23680..24978)
                     /gene="tig"
                     /locus_tag="ACHKUN_001862"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6660128.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(25322..25639)
                     /gene="bolA"
                     /locus_tag="ACHKUN_001863"
     CDS             complement(25322..25639)
                     /gene="bolA"
                     /locus_tag="ACHKUN_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO6660129.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            25944..26522
                     /gene="yajG"
                     /locus_tag="ACHKUN_001864"
     CDS             25944..26522
                     /gene="yajG"
                     /locus_tag="ACHKUN_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6660130.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            26566..28041
                     /gene="ampG"
                     /locus_tag="ACHKUN_001865"
     CDS             26566..28041
                     /gene="ampG"
                     /locus_tag="ACHKUN_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO6660131.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         6655 a         7653 c         6788 g         7017 t
ORIGIN      
        1 gtatggaaat ttattcgcta ataaataatt cgctttcgga gctataaccg gctgtttatt
       61 aagaatttta tactttttca ccatgaagac ataccctatg tgatctttat cacacagatg
      121 taataggaac gttctcttca ctgacttttc gtcttactgt gttgccgcat tttcagcaac
      181 cggaggtcag taatgagcac agaaaaagaa aagatgattg ctggtgagtt gtatcgctcg
      241 gcagatgaga cgttatctcg cgatcgcctg cgcgctcgtc agcttattca ccgatacaat
      301 cattccctgg cggaagagca cacattacgc cagcaaattc tcgctgatct attcggtcag
      361 gtgacagagg cttatattga gccaacgttt cgctgtgact atggctataa catttttctc
      421 ggtaataatt ttttcgccaa cttcgattgc gtgatgcttg atgtctgccc tattcgcatc
      481 ggtgataact gtatgttggc accaggcgtt catatctaca cggcaacaca tcccatcgac
      541 cctgtagcac gtaatagcgg tgctgaactg gggaaacccg tcaccatcgg taataacgtc
      601 tggattggcg gacgcgcggt cattaaccct ggtgtgacca ttggtgataa cgtcgtggta
      661 gcctcaggtg cagttgtcac aaaagatgtc ccggacaacg ttgtcgtggg cggtaatcca
      721 gtcagaataa ttaaaaaatt gtaatcggtt tttcgcaact gttatgcaaa attgtggtag
      781 atctgttact tcccctctac tattcccacg ttaaaatagg gtgttccctg gaaagttgca
      841 gataccacga aggcaaacga tgaccgaaat acaacgcctg ctgaccgaaa cgattgagtc
      901 tctgaatacc cgcgaaaaac gcgacaacaa accccgcttt agtatcagtt ttatccgtaa
      961 acatccgggg ctgtttatcg gtatgtacgt tgcttttttt gccaccctgg cggtgatgtt
     1021 gcagtccgaa acgctgtcag gctctgtctg gctactggtt gtattattta tcctgcttaa
     1081 tggtttcttc tttttcgatg tctacccacg ctaccgctat gaagatatcg acgtgctgga
     1141 tttccgcgtt tgctataacg gcgaatggta caacacgcgc tttgtacctg ccgcgctggt
     1201 tgaagccatc ttgaactctc cgcgtgtcgc ggatgttcat aaggaacaac tgcaaaaaat
     1261 gatcgtccgt aaaggtgaac tgtcttttta cgatattttt accctcgctc gcgccgaatc
     1321 aacatcttaa gttagggtta cataccaggc gtaaagctct gcgcctggtc aaatgacaat
     1381 gatcgtttcc acccatcact tcatgaaata ccagctctac ctccttatct cctgccagcc
     1441 tttttccaca atcagatata ctttccctac actgtgttaa taaggatatg ctggtgagaa
     1501 cacgacatct ggtcggcctt atttcgggag tactgattct ttcagtattg ctgcctgtcg
     1561 gcttaagcat ctggctggcc catcagcagg tagaaacatc gtttattgaa gagctggata
     1621 cctattcctc ccgcgtcgct attcgggcca ataaggtggc gacacaaggg aaagatgcgc
     1681 tgcaagagct ggaaagatgg caaggcgctg cctgtagcga agcccatctc atggaaatgc
     1741 gtcgggtatc ttacagttat cgctatattc aggaagtggt ttatatcgat aacaacgttc
     1801 cccagtgttc gtctctggag catgaaagtc cgcccgatac cttccccgag ccaggtaaaa
     1861 tttcgaaaga tggttatcgt gtctggttaa catcgcataa cgatttaggc attatccgtt
     1921 acatggtcgc catgggaacg gcacattatg tcgtcatgat cgaccccgct tcctttattg
     1981 atgtcattcc ctatagctca tggcaaattg atgccgccat tattggcaat gcccataacg
     2041 ttgtcataac cagcagcgat gaaattgctc agggaattat taccaggcta caaaaaacac
     2101 ccggtgagca tatcgaaaat aatggaatca tttacgatat cctgcccttc ccggagatga
     2161 atatttcgat catcacctgg gcttcaacga aaatgttgca gaaaggctgg catcggcaag
     2221 tctttatttg gttaccgctc gggttggtga ttggccagct ggcagcgatg tttgtgctgc
     2281 gtattttgcg ccgtatccag tcaccgcatc atcggctgca ggatgctatc gaaaatcgtg
     2341 atatttgcgt gcactatcag ccgattgtct ccttagccaa tggcaaaatt gtcggtgctg
     2401 aggcactggc gcgctggccg cagacagacg gtagttggtt gtcaccagat agttttattc
     2461 cgctggcaca gcaaacgggc ctttctgagc cattgacgct actgattata agaagcgtct
     2521 ttgaagatat gggcgactgg ctgcgtcagc atccacagca gcatatttcg atcaatcttg
     2581 aatccaccgt gctcacctcg gaaaaaatcc cgcaattgct gcgtgaaatg atcaatcact
     2641 atcaggttaa tcccagacag atcgcgcttg aactcactga acgcgagttt gccgatccga
     2701 aaaccagcgc cccgataatt tctcgctacc gggaggcggg ccatgaaatt tatcttgatg
     2761 attttggtac gggatattca agtttaagtt atttacagga tctggatgtc gacattctga
     2821 agatcgataa atctttcgtt gatgcgctgg aatataaaaa tgtcacgccg catatcatcg
     2881 aaatggcaaa aacactgaaa ctgaaaatgg tagcggaggg aatcgaaacc agtaaacaag
     2941 aagagtggct acgccagcat ggcgtgcact acggtcaggg ctggctctac agcaaggcat
     3001 taccgaaaga agatttctta cgctgggccg agcaacattt gtgaattttt gggctgacgg
     3061 cacgccgcac agcccgtcac gctacctatt cgtcgatgat tgactcgaat ccgccataga
     3121 tcattcgctt gccgtcaaac ggcatggact cgccgaactc tttcatccgt gggtccgaca
     3181 tcatcttttg attagcagcg tcgcggacct cttttgaagg gtattcaatc cagctaaaga
     3241 caacctcttc attctcttcc gctttcaccg ccatacgaaa atcggtcact ttgccatccg
     3301 gtacatcgct ggcccagcat tcgacaatac gaagcgcgcc aaactcttta aacaatggcg
     3361 cagccttagc ggccatttcc cgataggcat cctttttatc ggcaggaacg gcaaccacaa
     3421 aaccatcaac atacttcatc agacatacct ccgaagcgta ctgcgcagcc agacaattct
     3481 tgcgacgtca gttgtggtta aagtttagcc atgttggttt tttttgacga aacggtggga
     3541 ggcagaaatg ggtgggggcc ctgccagcta catcccggca cacgcgtcat ctgccttggc
     3601 tgcttccttc cggacctgac ctggtaaaca gagtagcgtt gcgggagaac caacaggccc
     3661 ccattgagag cgttgagaac caacgcgcag gcgcattatc actgctgccc cggctaattg
     3721 caacccatcg cgtgccagat gctggtttct tgcgcaatgc gacttcactc gggcgttttt
     3781 ccagattatg ctgaaaaatt acctcaggaa gaaaagatgg aaaaagaaga ttcatttccc
     3841 caacgcgtct ggcaaatcgt cgccgctatt cccgaaggct atgtcaccac ttacggtgat
     3901 gtggcgaaac tggcgggatc gccccgcgcc gcgcgccagg tgggcggtgt gttaaagcgt
     3961 ctccctgaag gcagcacctt accctggcac cgggtggtta atcgccacgg cacaatttcg
     4021 ctaaccggac cggatttaca gcgtcagcga caggcattac tggcagaagg tgtgatggta
     4081 tcgggaagcg ggcaaatcga cttgcagcgt tatcgctgga actactaacc ctctccgggc
     4141 ggagagggtt aacgtcgctt aatattgcgt aggagctgga actgccgaag acggattcac
     4201 ctgagttggt gaagtcgaag gtacggtagt cgttgcacca ccgctggcct gaaccggcac
     4261 ggcggtttgc tgcaccggca ccaatgtcag gtcggcttta gttccgccct tgttgatcac
     4321 cggctgaacg gtatcggtaa taaataccag tttgtcattc acggtaatcg ccgcactcaa
     4381 cagaatacgc gcgttcggct gaacatctgc cggattaaat ggcagaacaa agctgaatgg
     4441 tgactgttta ccttcggtac gcaccgcttt ctgcgccagc acttttgacg gtgcatcggc
     4501 taacgacgcg tcagaaagtg tcacggtcag cacagcatca ggcggcagtg cgactttctg
     4561 ccggatccag acggtaccgg agacattcgg ttgctggata gcaggttgtt gtgttgctga
     4621 aatagatgta tttgcagccg gggctggcgt ctgaatatcc gcgcttttat ctgcgcaagc
     4681 cgccaacgca atcgcaaccg ctaaaccact ggccatgtgc acgagtttca ttcatttctc
     4741 cttattatca atgcaccagc gggctaactt tcctcgccgg aagagtggtt aacaaaatag
     4801 taacgtcaac aagtgtggca cacatcacgc atttctgcct gtaattagcc cgtaattcag
     4861 accattgcac ccatcggacc agtagcaaat ttgcgttata ctcaactcac tttggcttgc
     4921 tgcggcagct ttgttactgg agagttatat gagtcaggcg ctaaaaaatt tactgacatt
     4981 gttaaatctg gaaaaaattg aggaaggact ctttcgcggc cagagtgaag atttaggttt
     5041 acgccaggtg tttggcggcc aggtcgtggg tcaggccttg tatgctgcaa aagagaccgt
     5101 ccctgaagag cggctggtac attcgtttca cagctacttt cttcgccctg gcgatagtaa
     5161 gaagccgatt atttatgatg tcgaaacgct gcgtgacggt aacagcttca gcgcccgccg
     5221 ggttgctgct attcaaaacg gcaaaccgat tttttatatg acggcctctt tccaggcacc
     5281 agaagcgggt ttcgaacatc aaaaaacaat gccgtccgcg ccagcgcctg atggcctgcc
     5341 ttcggaaacg caaatcgccc aatcgctggc gcacctgctg ccgccagtgc ttaaagataa
     5401 attcatctgc gatcgtccgc tggaagtccg tccggtggag tttcataacc cactgaaagg
     5461 tcacgtcgca gaaccacatc gtcaggtgtg gatccgcgca aatggtagcg tgccggatga
     5521 cctgcgcgtt catcagtatc tgctcggtta cgcttctgat cttaacttcc tgccggtagc
     5581 tctacagccg cacggcatcg gttttctcga accggggatt cagattgcca ccattgacca
     5641 ttccatgtgg ttccatcgcc cgtttaattt gaatgaatgg ctgctgtata gcgtggagag
     5701 cacctcggcg tccagcgcac gcggctttgt gcgcggtgag ttttataccc aggacggcgt
     5761 gctggttgcc tcgaccgttc aggaaggggt gatgcgtaat cacaattaaa aaaaatagcc
     5821 ggaggtaaaa ccgtccggct attttttgca gtgcttgtta cgcgttatag gcattctcgc
     5881 cgtgactgtt gacatccagc ccttctcgct cctgctcttc cggtacacgc agaccaaccg
     5941 tcagatccgc cagtttgtag ccaataaatg ccaccacacc ggaccagacg atcgtaatgg
     6001 cgatgctttc cagctgtacc agcaactgat ggcccatcgt cacaccttca gcgaagccca
     6061 cgccacccag cgaactggcg gcaaaaatcc cagtcatgat acagccgaca atgccacaaa
     6121 cgccgtgcac accgaagaca tcgcagggat catccacccg cagcaagcgt ttgagcatag
     6181 taacgcccca caagcccgcc agaccagcta ccacgccgat aatcaacgcg ccgccaaccc
     6241 caatgtagcc acacgctggc gtcacgccga ccagaccggc aatcgcgcca gaacacgctc
     6301 ccagcagaga aggcttacca cgcagcgccc attcaccgaa gatccagcca agaattgccg
     6361 ccgccgttgc gaccacagta ttcacaaatg ccagtgccgc gatttcattc gccgtgcccg
     6421 ctgacccggc gttaaagcca aaccaaccaa tatagagaat ggcagtaccg gtgaagacca
     6481 ttggcaggtt gtgcggttta aacgcctctt tgccgaagcc cacgcgtttg cctatcagat
     6541 acgcgcccac cagaccggcg attgcggcgt taatatgcac caccgtgcca cccgcgaaat
     6601 ccagcgcacc gtgagaagcc agcaaaccac cgccccacac catatgcgca atcggaatgt
     6661 aagagagcgt cagccatacc accacgaaaa tcaacacagc tgagaagcgg attcgttccg
     6721 ccagcgcccc aacgatcaag ccgacggtaa tgcaggcaaa cgagccctga aacgccacgt
     6781 ggatatactg ataaatgctg cccatcaccg ccgtcagttc gatgtttttt agcatcaacc
     6841 agttaatgtt gccgaagaag ttgttgccct caccaaacgc cagcgagtaa ccgtaaacca
     6901 cccagagaat acagaccagt gcaaacgtca ccgtcacctg cgtcagcatc gacagcacgt
     6961 ttttgccgcg aatcaaccca ccgtaaaaca gggcaatccc cggaatagtc ataaacagca
     7021 ccagcgcagt acaaatcatc ataaacgcat tgtcggcttt atcggccacc gcaggtgcag
     7081 ccattaccag tcccggcagc atcgccagtg aagcaagccc agtttttatc gtcgctatct
     7141 tcatttttcg ttcctgttgc tgtgtgccag agattacagc gccgcttcgt cggcttcgcc
     7201 ggtacgaata cgaatgacgc gttgcaattc agcgacgaag attttgccgt cgccaatttt
     7261 tccggtgtaa gccgccttac tgacgatatc gatcacttca tcgagttggt catcagcaat
     7321 cgccacatca atttttactt ttggcaggaa gttgacgctg tattccgccc cccggtacag
     7381 ctcggcatgc cctttctgac gcccgaaacc tttcacttcg gtgacggtca ggccctgaat
     7441 accaatggaa gataacgctt cacgaacgtc ttccagcttg aatggtttga ttatcacggt
     7501 caccagcttc atagatcccc tccggtcaga attcggtaat ggttctgcta cacgaagaag
     7561 gtattgcaag cggtgtgcca gaaatgaaaa acgagaaaga gagcaggaag ttaagaagat
     7621 tgaaaatgaa aacgcactat gacagtgcac agtgcgttac atttttgcac caacgatgaa
     7681 atattgcgct attcaggcgc tcaatgattc ttcttcacgc acgctggctg ccagctcttc
     7741 gcccgcaagt tgcagttgat acatctgcca gtagcgtccc tgggccgcca gcagttgctg
     7801 gtgagtgccc tgctccacgg cttgcccacg atgaagcacc agaatggtgt cggcatcaac
     7861 aatggtcgat aagcggtgag caatcaccac cagcgtggta tgttcacgca cagccgccag
     7921 agcatgttga atcgcctgtt cagtaccgga gtcaatgctg gcggttgcct catcaaggat
     7981 caggatttgc ggcgtctcca ccaacacgcg tgccagtgcc agcagttgct tttgcccgac
     8041 ggagagatta ttcccctgct cgcccagcgg cgtataaata ccatcactca tgctgcgcgc
     8101 cagctccgcc agttgcacgg tttccagcgc ctgccagacg cgttcttcgg agatatcacg
     8161 ccccagcgtc acgttggcga ggaaggtatc cgccagcacc accggatctt gctgcaccat
     8221 tgccacgccc tggcgcagcg cgctgtgact tagcgaactt aatggacgac catcaaggcg
     8281 aatctcacct tccgttagcg ggtaatagcc catcaataaa ctggcgaggg tgcttttgcc
     8341 actgccggta tgcccgacca gtgccacaaa gttacgcgaa ggcacagaga gattaatgtt
     8401 ctttagcacc agattgtcat cgcgataagc aaatgacacg ttatcgactt cgatggtgcc
     8461 actctgtaac gggcgatcat cattgccata ttgctggcgc ggtccgtcca tcagttcaaa
     8521 cacgcgctca ccagcaacaa cagcctgttg cagcatcgcc tgttgcgtgg tcagttcgat
     8581 taatggttcg ttaagtcgcc caagatagct gataaacgca tacagcacgc ccacttcaat
     8641 ggtgccgcta gcggagaagc caaacagcat caacaagcca caaagaatga gcgatgaaaa
     8701 cagactcagc agcggacgca gcagaaaacc gtcgaggcgc agggtttgca tcctggccat
     8761 atagtgtgaa cgactggcct cccccatacg ttcgccaaat cgcgcctgct gacggaactg
     8821 ctggataacg ctcatgccat tgatgatttc gttaaagccg tcgttgatat ccgctagata
     8881 ggcgcgcaca cgacggacaa tcggcgtgct gtaacgctgg tatatcacca ttaccaccag
     8941 caccaccggg aaaatcatta tcgccaccag cgccattcgc cagtcgaggc tgaacatcgc
     9001 caccagcatc gcgcccacca gtgcggcgct acgcaggaca gttgccacta cggtaacgta
     9061 gagatcgcgg atcacttcag tgtcattagt gacgcgggaa atcacctgcc cgacgggttg
     9121 ggtatcaaac tcgcttaatg gctggcgcaa tgcggcatcc atcacgtcgg tacgcaactg
     9181 ttgcactacg cctactgccg cccgattaaa cagcagcgac tgcgcgtaat gtagcccggc
     9241 ggcaaacagt tgtagcccaa catacgccgc agccagccct gcaaccattt tcaacggcag
     9301 gttatttttc gctaccatat tgtcgataaa atagctgata agcagcggcc cactgacttc
     9361 tgccgctgcc gcaacccaca tcatcaggac cgcaatcccc agcggtttac gccacggcga
     9421 accgtacgct aacagacgct tgagagtcgg ccacagttgg ctaaaactac gcatcgacgg
     9481 cctcctcgcg aatttccgga gcgtcgtcga gcgccgcctc cagttgttga tagcgataca
     9541 tatcgcgata ccagccgctt tgttgcgcca gctcatcatg attgccacgc tgggcgatat
     9601 gtccgtgctg catcacaata atttcactgg cttccgtcag tgccgaaagg cgatgggcac
     9661 tgatgattac cgttctaccc tgtccccact gacgcaggtt atgcaggatc tggtgctctg
     9721 tgcgtccgtc caccgccgaa agcgcatcat caaggatgag gatttccgcg ttgactaata
     9781 acgcacgagc aatagagata cgctgttttt gcccgccgga aagcatcacg ccgcgctcgc
     9841 ccacctctgt atcgtaacct tgcggtaaac gcaaaatatc gtcatgtacg ctggctaacc
     9901 gcgcgacatg ctcaatctct tgctgggtgg cattcgggca acccaacgcg atgttgttcg
     9961 ctacggtgtc ggaaaaaagg aacggtgtct ggctaactac cgccaggcgg ctacgccagc
    10021 tatcgagttg taacttcgtc agaggaatat catgaaagcg aatatccccc tcgctgacgt
    10081 cgaaatgacg ctgaatgagc gacaacaggg tacttttgcc ggaaccagtc ggcccgcaga
    10141 tacccagcat ctgaccgggt ttcagggcga aattgacgtt ttccagcgca cgatggtcag
    10201 tctgcggata cgtgaactgg tgaatattta catcaagttc gccacgccct tccggcacgg
    10261 gttcgctacc atcgttcacc accggcgctt ccgccagcat cgcacgaata cggctgtacg
    10321 cagcactacc acgttccaca atgttaaaca tccatgccag cgccagcatt ggccaaatca
    10381 tcagacctaa atacatcata aaactggtga gctggcccag cgttaaactg ccctgcacca
    10441 ccatccaact accgccgcca atcgccagca agttcgccat accaatcgcg atatagattg
    10501 tcgggtcgaa acgagcatca atacgcgcca cccgcatgtt ttttttgccg gtatcttcgg
    10561 catccgcggc aaataacgcc gactggcgat cttccagacc aaaggctttg atcatgcgga
    10621 tactggtgag gctttcctgg gtgcggtcat taagactgga aaacgccgcc tgtgccagct
    10681 taaagcgttc atgcaaggca tcgccgttgc gcttaatcat gatcgccatc actggcatcg
    10741 gtaacagggc aaataaggtc aactgccagc taatttgcgt agacatcata atcaacacag
    10801 cgcagcccat taccagtgaa tccaccagcg tcagcacccc ttctccggcg gcaaacacga
    10861 cgcgatcgac gtcattggtc gcacgagcca tgagatcacc cgtgcgatga cgcaggtaaa
    10921 actccgggtg ctgccggctt agctgacggt aataatcttc acgcagttca acagccagtt
    10981 ggtaagacgc gccaaacagc aatacgcgcc agacataacg caggagataa accacaacgg
    11041 caatcagcac catggtggcg atccacatca ggatcagccc ggtagtaaag tgttgttctg
    11101 tcacgccatc gacaacaata ccaaccactt ttggcggaac cagttgcagc atcgcgataa
    11161 tgacaagcaa ggcgacagcc ccgagatagc gacgccattc ccgacggaaa taccagctta
    11221 attgagcaaa taatcgcacg cgttgtgtcc tgacctgatt ctggatattt attcgatggg
    11281 taaagaagtg gtgtatttaa tctgttccat cgcgaagctg gaagtgacgt ccgacagccc
    11341 cggcacgctg tttaccagac gcttataaaa ctcgtcgtag cgtttcatgt cggcaacctg
    11401 gacgcgcatc agataatcgt attcaccagc catgcgccag aaccccagca cttctggcat
    11461 ttcggtaacc accgtgacaa agcggcaata ccattcgctg ctgtgatgtt gcgtttttat
    11521 cagcacaaaa gcggtcaggc cgaggcctat tttttccgga tccagcaggg cgactttgcc
    11581 gataaggata ccgtcgtcct ccagccgttt cagacgcttc cagcaagggg tggttgtcag
    11641 attaacggct tcagccagtg cctgcaaaga gagggtgcaa tcctgctgca gtaaggccag
    11701 cagcttatgg tcaattttat ctaacatagc ccttccacag agaatttttt tctcttttaa
    11761 ttctatttta aaggtgaaat cacgacaatt tattctgtgc ggtttaaaca ccacggcaca
    11821 aaatgacaaa ttcaacgcaa aaaaaaccgg gcaattgccc ggtgctggaa gggatctcgt
    11881 tattcggggg aataaggtag atgtggatag tccagccagt gcgtcaaata gtgagagaca
    11941 gcctgatttc ggcaatgtcc aatcaccggt aaatgcggga gctccgcgcg cagttgcggc
    12001 atcgcattgc ccataataaa cccgctaccg acgctgccta acatttcgcg atcgttcatc
    12061 gcatcaccaa aggccatgca atcgcgcaac gataaaccta aatgttgggt cagcaccgtc
    12121 aatgcagcgc ctttattgca gcccaccggc agcacttcga ggcaatccgt ggcggaaaaa
    12181 cacaaatgtg cacgctcgcc taatgcttcg tatagctgga tctgcaagcg tgtaagatcg
    12241 tcgtgaccgc cacagaagca gatcttggtg actctgccga gtggcatttt tttgacatcg
    12301 attatctgat aacgaaaacc gctatagaca aatgcctgca acaacgcagg gatctctttc
    12361 ccggtaaacc aaccgtcgtc attgaagata tgcatgctgg ctcgggtatc ccattgctga
    12421 tacagcacca gctccgcgac atccgcaggt aaatcatcac gatgtaaaag ttcaccttcc
    12481 agagaatgca cgcgcgttcc gttgccggta atcaaatatg catccagcga tagcgccccg
    12541 agaatatgct gcatctccag cgcatgacgc cccgtggcaa aagtgagggt aatgtcgcgt
    12601 tcacgcagtc gcgccaaagt agagagggtt ttctcaccta aatgatggtc aggcatcaat
    12661 aaagtgccat ccatatcaaa tgctgccaga cgagccattt ctttctccac tctgtgacac
    12721 ggttaatggt gattgagtat cacctgatat atacggaagt aatagtgaat agttaaataa
    12781 tattgttccg ggtttatatg cgattgctca accgtcttaa ccagtatcaa cgtctgtggc
    12841 aaccttcagc cggaaagccg caaaccgtca ccgtcagcga actggccgaa cgctgttttt
    12901 gcagcgaacg ccatgttcgt acgctgttgc gtcaggcaca ggaggcggga tggctggagt
    12961 ggcaggcgca gtcaggacgc ggaaagcgcg gacaattacg ctttctggtc acgccggaat
    13021 cgctacgcaa tgcgatgatg gaacaggcac tggaaaccgg aaagcagcaa gatgtgctgg
    13081 agctggcgca actggcccca ggtgagctgc gcactctgtt acagccgttt atgggcggac
    13141 aatggcaaaa cgatacaccc acgttgcgta ttccctacta tcgcccgctc gaaccgctac
    13201 aaccaggctt tttgcccggc cgtgccgagc agcatctcgc cgggcagata ttttccggcc
    13261 tgacccgctt cgataataat actcagcgcc cgattggcga tttagcgcat cactgggaaa
    13321 cctctactga cgggttacgc tgggactttt atcttcgttc aaccctacac tggcataacg
    13381 gcgatgcagt aaaagcctca cacttacacc agcgattatt gatgctgtta caactgccag
    13441 cactggatca attatttatt agcgtgaagc atattgaagt cacccatccg cagtgtctga
    13501 ccttcttttt acatcgcccc gattactggc ttgcgcaccg gctggcgagc tattgcagcc
    13561 atctggcgca tccgcaattc ccactgatcg gcactggtcc ttttcgctta acacaattca
    13621 cagcggagct ggtgcgcctg gagagccatg attattacca tttgcgtcat ccgctgctta
    13681 aagcggttga gtactggata actccgccgc ttttcgaaaa agatttggga aacagttgtc
    13741 ggcatcccgt gcaaatcacc atcggcaaac cggaggagct gcaacgggtc agccaggtca
    13801 gtagtggcat cagtttaggt ttttgctatt taacgttgcg caaaagtccc cgactctccc
    13861 tctggcaggc gcgaaaagtg atctccatta ttcatcaatc cggtttatta caaacgttag
    13921 aagtcggaga aaacctgatc accgccagtc atgcattact gccaggctgg actattccgt
    13981 actggcaagt accggatgaa gtcaaactac cgaaaacctt gacgctggtt tatcacctac
    14041 cgatagaact tcataccatg gcagaacgcc tacaggcgac actggcagcg gaaggctgtg
    14101 aactcacaat tatttttcat aacgcaaaaa actgggacga cacgacccta ctggcacacg
    14161 cagacctcat gatgggcgac agattaattg gcgaagcacc ggaatatact ctggagcaat
    14221 ggctacgttg cgatccacta tggccacatg ttttcgacgc tccagcatat gcacatctgc
    14281 aatcgacgct ggacgcggtg caagtaatgc ctgatgagga aaaccgattt aatgccctga
    14341 aagcggtttt tagccagtta atggcagacg cgacgctgac gccgctgttc aactatcact
    14401 atcgcattag tgcccctccc ggcgtgaacg gtgtgcgact gacaccgcgc ggctggtttg
    14461 aatttaccga agcctggctt cccgcgccgt cgcaatgaat agctggtccg ggttaatcgc
    14521 aggcgttacc ataacgcttt tattgttcaa gcaggattat ctatgaaacg tgctgtcgtt
    14581 gtgttcagtg gaggccagga ttccaccacc tgtctggtgc aggcattaca acaatatgat
    14641 gaagtccatt gcgtgacgtt cgattacggt cagcgacatc gcgcagaaat cgacgtggca
    14701 cgcgaactgg cgctgaaact gggagcacgc gcgcataagg ttcttgatgt aacgctgctc
    14761 aacgagctgg cggtcagcag cctgacacgt gacagcattc cggtgcctga ttatgaacct
    14821 gaagccgatg gcatcccgaa tacgtttgtc cctgggcgta atattttgtt cctgacgctg
    14881 gcggcaatat atgcgtatca ggtaaaagca gaagccgtaa ttaccggcgt ctgtgaaacg
    14941 gatttctccg gctacccgga ttgccgcgat gagtttgtga aagcactaaa ccatgccgtc
    15001 agtttgggca tggcgaaaga tattcgtttt gaaacgccgc tgatgtggat tgataaagcg
    15061 gaaacctggg cgctggcaga ttattacggc aaactggatt tagtccgtaa cgaaacgttg
    15121 acctgctata acggctttaa aggcgacggt tgcggtcatt gtgcggcatg taatttacgc
    15181 gccaacggtt tgaatcatta tctggccgat aaaccgacgg tgatggcagc gatgaagcag
    15241 aaaaccgggt tgaggtaatt attccgggtg tcgccggatg cggcttgagc atccggcacc
    15301 acaaaacgtt tacttaacca tctgctccag cttttcgcgc aattcccctt ccagagctaa
    15361 tgctttctgc gttttaagat caatacaaac aaacgtaata agcgcatccg ctaccacctg
    15421 cccttccggc tccagtgtaa tgacctggct taagatgccg cttttaccgt ttaattgctg
    15481 caactgactg gtaatagtta acaggtcgct taataccgct gggcgacgat agttaatatt
    15541 gatattgacc acgacgaagg caatgttatg ggccgtcatc cactgaaaac tgtcgctatt
    15601 ttccaaccca tcccagcggg cttcttcgag aaattcaagg tagcgggcgt tgttgacgtg
    15661 ctggtaaacg tcgagatgat atccacgaac tttgatttgt gtttgcatag cgcaataacc
    15721 ttacgttgtt gttataagta cagaggtcat aactggtatg acctcttcag tctggcaaaa
    15781 aattgccact atgcaaatta attacagggt taataccgcc agattacgtt ccaccagcga
    15841 attgcccatc cccggcacct gctttagatc ctccaccgtt ttaaacggac cgtactcttc
    15901 gcgataactg acaatcgcct gcgctttctt caggccaacg ccattcatcg cgcgggctag
    15961 ctcttccgcg ctggcattat taatgctgac ccgggtgcct tcttcgtcac tggctttcgc
    16021 cggtactgct gctttacttt gtgctgcagg agcttccgct ttggtttcta ccgccgtcgg
    16081 tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg ccagggacag
    16141 ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt tgttaacagt
    16201 ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga aatggaaaag
    16261 gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga ggcggcttca
    16321 ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac gcaggttact
    16381 catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca ccatcgcttt
    16441 tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca gaaccacatt
    16501 accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca gtggcagtgc
    16561 aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aagattttcg gctcgccaaa
    16621 tttcagaccg gcagcctgca tagcttccgc acctttgccg gctttcaaat caaccagcag
    16681 tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca gtgccttaac
    16741 ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc tgatgcgcag
    16801 cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg gcgcgccgtt
    16861 ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt tcaactcttc
    16921 cggcaggtta tctttgctaa accaacccgt ctgagtggct ttaacgccgg cagcttgctc
    16981 tgcaccggcc agagactccg tgtcgttgct tgccgcatcg ctcactttct gctgcagcgc
    17041 atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt cacgtacttc
    17101 gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca ggaaaccgac
    17161 cgaagatttg atgacaccag acagttgccc tttttctttc agaccagcat ttttcagttc
    17221 gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg cagagataat
    17281 atcggcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca gctcatcaag
    17341 taccactttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg tacgctgcgg
    17401 ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat cgctaaccgg
    17461 ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt ccggcgtcat
    17521 gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca caggctgctt
    17581 cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt gcgcgaccag
    17641 tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa caccgttaat
    17701 cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg cggtcatccc
    17761 catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa cctggaaggc
    17821 tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt tcagctcacg
    17881 agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt gacgcagggt
    17941 tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct gttgctgcat
    18001 gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt cctggtcatt
    18061 cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc cggtcaatat
    18121 gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg cagccgtgcg
    18181 taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac ctcacgcaac
    18241 gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc agcccacaag
    18301 acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc ccttgaactt
    18361 cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc tttacctgca
    18421 cggaagctcg gtactttagc agcagcgatg gtgatctctt taccggtctg cgggttgcgg
    18481 ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag tgctacatca
    18541 tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc acggccagcc
    18601 gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga tttattcact
    18661 cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg aacgcgcagc
    18721 agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag cccgatccgc
    18781 catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc cagccctgtt
    18841 tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca tgccagacgg
    18901 ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat gaatgtccag
    18961 atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga acggaattag
    19021 cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac caccgatcgg
    19081 cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac gaaccgggtt
    19141 accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg gaccatcttt
    19201 cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa aatcagggtt
    19261 gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa tagactcctg
    19321 catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa cacatgcggt
    19381 ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta cctgaccgac
    19441 acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt agtcatgcag
    19501 gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact gcttaaccgc
    19561 tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct cacgggtgta
    19621 gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt ttttcagtgc
    19681 attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt tttcatcttc
    19741 ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg gaatgttcat
    19801 ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt ccaggtagtg
    19861 gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag aggccggatc
    19921 gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg ggtttttcac
    19981 gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt aagtacggcg
    20041 gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga catatttacg
    20101 cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg gcggccctac
    20161 caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa ggtactcaag
    20221 gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt cctgcgcctg
    20281 acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca tccagtcgat
    20341 ataaccacgc actacggtcg cttctgccga catcggagac atcattttca gcttctgcaa
    20401 ctctgcttcc gctttctctt ttgcctcttt cggcattttc gccgcgtcga ttttgcgctt
    20461 cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct gaatagcttt
    20521 catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt taacgcggtt
    20581 gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg ccatcagata
    20641 ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag ccagtttcag
    20701 cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc tattcagcga
    20761 cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga actggctgat
    20821 tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact ccagatactc
    20881 cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg cgcgctgtaa
    20941 cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca atatagaggc
    21001 cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag cttctttctg
    21061 cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac ggatagattt
    21121 ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat cgcgcagcgg
    21181 caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct ctcttagttt
    21241 aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt aacatgtacg
    21301 tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa aattaaaggg
    21361 gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat tattcaccag
    21421 atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga ccatcaatta
    21481 ccgactcgtc gataaccact ttttcgacat cttccatgga cggcagatcg tacatggtat
    21541 cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta cgcgccatcg
    21601 ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg ccttccagat
    21661 taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg atctgaatca
    21721 gagcttcttc gctcagttca ttcaacgttg cgacaaccgg cagacgacca ataaactcag
    21781 ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc tcgccttcgc
    21841 ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag ccggtttcta
    21901 cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata aacaggatct
    21961 tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct tgcggtggaa
    22021 cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct tcaccggaaa
    22081 cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatc tcatcgatgt
    22141 agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttctgcaac agcttctgaa
    22201 tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt gcgtcggcca
    22261 tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt ttaccggaac
    22321 cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg ctggtatcgc
    22381 cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt ttcgcctgtt
    22441 cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc ggtagcgcac
    22501 tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg ttacataaat
    22561 caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc acttcatgct
    22621 ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg cgtttatctg
    22681 tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca aatgccattc
    22741 tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg cacagttgcg
    22801 cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt ccaccgcttc
    22861 aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta atgattgacc
    22921 cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt cacgggcatg
    22981 aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa tcatcacgcg
    23041 cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcaacaaga aagcgcccat
    23101 cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact gcatggtgtc
    23161 atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca gatagatatc
    23221 tttttctggg ttttccgctt ccaggaacag catctgcgcc acaatcaggt tagccatgtg
    23281 gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag aatagatatc
    23341 aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg ccatatgggg
    23401 tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa aattgaaaaa
    23461 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    23521 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    23581 cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa agcccaccac ctggcggtga
    23641 cgggcctttg tgcgaattta gcgcgttatg ctgcgtaaat tacgcctgct ggttcatcag
    23701 ctcgttgaaa gtggtttctt tttcagtcac tttcgctttc gccagtacag cttcaacagc
    23761 ctgttcttcc agagcaacat tgcgcatgtt gtccatcagt tctttgtttt tgctgtagaa
    23821 ctcgataact tctttcggat cttcgtacgc agaagccatc tcttcgatca ggcctttcac
    23881 gcgctcttcg tcagctttca gctcgttggt gcggataact tcgcccagca gcaggccaac
    23941 aactacgcgg cgtttagcct gttcttcgaa cagttcgcgc ggcagttcca gagcttgttt
    24001 ttcgttgcca ccgaaacgct gtgcagcctg gcgacgcaga acgtcgattt cgctgtcgat
    24061 cagcgcagcc ggtacgtcga tgtcgttagc ttttaccaga ccttcgatcg cctgagactt
    24121 aacgcggtta cggatggcgc tcttcagctc gcgctccatg tttttacgca cttcagcgcg
    24181 cagaccttct acggaaccat cttcaacgcc gaaacgtttg atgaattctg cagtcagttc
    24241 cggcagttca cgctcttcaa ctttcttcag gttgatagcg aatttcgctg ctttaccttt
    24301 caggttttct gcgtggtatt cttccgggaa ggtcacgtcg atggtgaact cttcgccagc
    24361 tttgtggcct ttgataccgt cttcaaagcc cgggatcata cgaccctggc ccatcgccag
    24421 tacgaaatca gacgctttac cgccttcgaa ctcttcgccg tctacagaac cggtgaagtc
    24481 gatggttacg cggtcttctg cttcaacagc gccgtctttt tctttccagg tcgcctgctg
    24541 tttacgcaga gtatccagca tgccgtcaac gtcagcgtcg gtcacttcaa cgatcggttt
    24601 ttcaacttcg atcgcttcca gaccctgcag ttcaacttcc ggataaactt caaactctac
    24661 agagtaagtg aagtcttcac ccagcttgta ttcgcccgga acataagtcg gtgcgccagc
    24721 cggattgatt ttttctttaa tgatggcgtc aatgaagtta cggctcatca ggtcacccag
    24781 aacgtcctgg cgtacagacg cgccataacg ctgagcaacg atattcattg gcactttgcc
    24841 tttgcggaag ccgtcaatac gtactttttt cgcaacgttg accagctcgc ttttaacagc
    24901 ggtctcgatg ctgtcagcag cgatagtaat cgttacacgg cggccaaggc cttgagtggt
    24961 ttcaactgaa acttgcatct tgttacctca aaaaatcaca gtgctcggtc aactcttacc
    25021 ccgcaagcac aggttgttgg cttcgcggaa ccgggatgtt cttataatca atcacattcc
    25081 ctgttaccag aatcatcccg aaagcattca aataggacgc ggcattatag cggcatcact
    25141 tttatgagtc gagaacggtt attgcgcgct gtcgcgtatt ttttcacaat tcccgggcaa
    25201 ttttcgtctg aatgctgggc aaaaagcgca aaatccagac aaaacaaaac ggcccgcagg
    25261 ccgtttttca taaaatcttt agcaacatac tggaaaagcg ccgacagttg caaatgcgtt
    25321 tttacgcgat gcttcctgct ccacgacagg gaggagaggc aaagacggtg tcctgcaacc
    25381 cttcccactc cttaatagtg taagtatgca gagccagcgc atgaacggta gtagagagtt
    25441 cctccgctaa agtactgtaa atcattcgat gacgattcag aaaacgttca cccgtaaaac
    25501 gatcgctgac cagcacaact ttaaaatggc tttcagagcc ggctgggaca ttgtgacgat
    25561 agctttcatc cactacttcg aggaatacgg gttggaacgc cgcccttaat ttttcttcta
    25621 tccgctcacg tatcatcatg aaatatctcc tccgacaacg ctgagatgtc atccatccct
    25681 ttaaatacta gccgctttta ccgtttccat tgcagcttaa caacaaatat ttaggtttcg
    25741 tcctggtttt tccgtcaagc gtatgaagtt tactgattta aagattgaaa cacaggcctc
    25801 gcatcagcgt ttcttttacc aggggtgatt atttcgtcaa catgatgaat ttacatgcct
    25861 tacccacttc ccctcgccgt tggcaatgtt atgatggcgg gaatttttac caaacacgct
    25921 taagagtctc tgagagcccg aacatgttca aaaaaatcct cttcccgtta gttgctctgt
    25981 ttatgcttgc aggatgcgca aaaccgccaa caactattga agtttccccg acgattacac
    26041 tgccacagca ggatccaagc ctgatgggcg tcaccgtaag cattaatggt gccgatcagc
    26101 gtaccgatca ggcgctggca aaagtcaccc gcgataatca aatcgttacc ctgaccgcct
    26161 cccgcgatct gcgtttcctg ctgcaagaag tgctggaaaa acagatgacc gcgcgtggtt
    26221 acatggttgg tccgaatggc ccggttaatc tgcaaatcat cgttagccaa ctgtatgctg
    26281 acgtgtccca gggcaacgtg cgctacaaca tcgctaccaa agcggatatc gccatcatcg
    26341 ctaccgcgca gaatggcaac aaaatgacca aaaactatcg tgccagctac aatgttgaag
    26401 gtgcgttcca ggcctccaac aaaaatatcg ccgatgcggt taacagtgtg ctgaccgata
    26461 ccatcgctga tatgtctcag gacaccagca ttcacgaatt catcaagcag aacgcgcgtt
    26521 aatttctgcc ctctggcccg gtgcaagccg ggcctgtaga cgcccatgtc cagtcaatat
    26581 ttacgtattt ttcaacagcc gcgttcagcc atattgctga tcctgggttt tgcttccggg
    26641 ctaccgctcg ccctgacatc cggcacctta caggcctgga tgacggtcga gaatattgat
    26701 ctcaaaacca ttggtttctt ctctctggta ggccaggctt acgtttttaa attcctctgg
    26761 tcaccgctga tggaccgcta cacgccccca ttttttgggc ggcggcgcgg ttggctgctc
    26821 gccacgcaaa tcctgttatt agtcgccatt gcggcgatgg gttttctcga acctggcacc
    26881 caactccgct ggatggcggc actggcggtg gtgatcgctt tttgctcggc ctcccaggat
    26941 attgtcttcg atgcgtggaa aaccgatgtg ctcccggcag aagaacgtgg tgcgggcgcg
    27001 gcaatcagcg tgctgggtta ccgtttaggg atgctggttt ccggcggctt agccctgtgg
    27061 ctggcagata aatggctggg ctggcagggc atgtactggt tgatggcggc gctgttgatc
    27121 ccttgtatta tcgcgacgtt gcttgcacca gaaccgaccg acaccattcc tgtgccaaaa
    27181 acgctggaac aagcggttgt tgcacctctg cgagatttct ttggtcgcaa taatgcctgg
    27241 cttattttgc ttcttatcgt gctgtataag ctgggcgacg cattcgccat gagcctgaca
    27301 accacgtttt tgattcgcgg cgtcgggttt gatgcgggtg aagtaggcgt ggttaacaaa
    27361 acgcttggct tactggcgac cattgtgggc gcattgtacg gtgggatttt gatgcagcgc
    27421 ctgtcgctgt tccgggcgct gctgattttc ggcattttac aaggtgcgtc taacgctggt
    27481 tactggctgc tgtcgattac tgataagcat ctctacagca tgggcgcagc cgtctttttc
    27541 gaaaacctct gtggcgggat gggcacatca gcctttgtcg cgctgttaat gacgctgtgt
    27601 aataagtcat tttccgcaac tcaatttgcc ctgctctcag cgctttccgc tgtaggacga
    27661 gtttatgtcg gccccgtggc gggttggttt gttgaagcac acggctggtc gacattctat
    27721 ctattctccg tcgccgctgc cgtaccgggg cttattttgc tgctggtttg ccgccagacg
    27781 cttgaatata caggagtaaa tgacaacttt atctcccgta ccgaatatcc ggcaggttat
    27841 gcctttgcca tgtggacact ggcggcgggc gtcagcctgt tggccgtgtg gttactgctg
    27901 ttgacgatgg acgcgctgga tttgacgcac ttctctttcc tgcctgctct gctggaagtc
    27961 ggggttttag tcgccctttc tggcgtcgtg cttggtggtt tgctggatta tctggcgcta
    28021 cgaaaaacgc atctgacgta atctgtaaat attatttagc tgtagtaatc actcgccgta
    28081 attattacgg ctaaataaac atcagtatcg ctt
//
LOCUS       DAZFRJ010000049        26609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000049 DAZFRJ010000000
VERSION     DAZFRJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.049"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..455
                     /gene="tnpA"
                     /locus_tag="ACHKUN_001866"
     CDS             24..455
                     /gene="tnpA"
                     /locus_tag="ACHKUN_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO6660132.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(471..659)
                     /locus_tag="ACHKUN_001867"
     CDS             complement(471..659)
                     /locus_tag="ACHKUN_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO6660133.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(663..1022)
                     /gene="yeaR"
                     /locus_tag="ACHKUN_001868"
     CDS             complement(663..1022)
                     /gene="yeaR"
                     /locus_tag="ACHKUN_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO6660134.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1195..1833)
                     /gene="leuE"
                     /locus_tag="ACHKUN_001869"
     CDS             complement(1195..1833)
                     /gene="leuE"
                     /locus_tag="ACHKUN_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO6660135.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1960..2883)
                     /gene="dmlR"
                     /locus_tag="ACHKUN_001870"
     CDS             complement(1960..2883)
                     /gene="dmlR"
                     /locus_tag="ACHKUN_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO6660136.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2986..4071
                     /gene="dmlA"
                     /locus_tag="ACHKUN_001871"
     CDS             2986..4071
                     /gene="dmlA"
                     /locus_tag="ACHKUN_001871"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO6660137.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4322..5932
                     /gene="yeaV"
                     /locus_tag="ACHKUN_001872"
     CDS             4322..5932
                     /gene="yeaV"
                     /locus_tag="ACHKUN_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO6660138.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5964..7088
                     /gene="yeaW"
                     /locus_tag="ACHKUN_001873"
     CDS             5964..7088
                     /gene="yeaW"
                     /locus_tag="ACHKUN_001873"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO6660139.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7144..8109
                     /gene="yeaX"
                     /locus_tag="ACHKUN_001874"
     CDS             7144..8109
                     /gene="yeaX"
                     /locus_tag="ACHKUN_001874"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO6660140.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8163..9278)
                     /gene="rnd"
                     /locus_tag="ACHKUN_001875"
     CDS             complement(8163..9278)
                     /gene="rnd"
                     /locus_tag="ACHKUN_001875"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO6660141.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9360..11045)
                     /gene="fadD"
                     /locus_tag="ACHKUN_001876"
     CDS             complement(9360..11045)
                     /gene="fadD"
                     /locus_tag="ACHKUN_001876"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO6660142.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11250..11831)
                     /gene="yeaY"
                     /locus_tag="ACHKUN_001877"
     CDS             complement(11250..11831)
                     /gene="yeaY"
                     /locus_tag="ACHKUN_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO6660143.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11871..12566)
                     /gene="tsaB"
                     /locus_tag="ACHKUN_001878"
     CDS             complement(11871..12566)
                     /gene="tsaB"
                     /locus_tag="ACHKUN_001878"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO6660144.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12624..14534)
                     /gene="yoaA"
                     /locus_tag="ACHKUN_001879"
     CDS             complement(12624..14534)
                     /gene="yoaA"
                     /locus_tag="ACHKUN_001879"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO6660145.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14666..15010
                     /gene="yoaB"
                     /locus_tag="ACHKUN_001880"
     CDS             14666..15010
                     /gene="yoaB"
                     /locus_tag="ACHKUN_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6660146.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            15433..15732
                     /gene="yoaC"
                     /locus_tag="ACHKUN_001881"
     CDS             15433..15732
                     /gene="yoaC"
                     /locus_tag="ACHKUN_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO6660147.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(15852..16031)
                     /gene="yoaH"
                     /locus_tag="ACHKUN_001882"
     CDS             complement(15852..16031)
                     /gene="yoaH"
                     /locus_tag="ACHKUN_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO6660148.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16105..17466
                     /gene="pabB"
                     /locus_tag="ACHKUN_001883"
     CDS             16105..17466
                     /gene="pabB"
                     /locus_tag="ACHKUN_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO6660149.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            17470..18048
                     /gene="nudL"
                     /locus_tag="ACHKUN_001884"
     CDS             17470..18048
                     /gene="nudL"
                     /locus_tag="ACHKUN_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO6660150.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18232..19596
                     /gene="sdaA"
                     /locus_tag="ACHKUN_001885"
     CDS             18232..19596
                     /gene="sdaA"
                     /locus_tag="ACHKUN_001885"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6660151.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            19727..21325
                     /gene="pdeD"
                     /locus_tag="ACHKUN_001886"
     CDS             19727..21325
                     /gene="pdeD"
                     /locus_tag="ACHKUN_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6660152.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(21329..22885)
                     /gene="yoaE"
                     /locus_tag="ACHKUN_001887"
     CDS             complement(21329..22885)
                     /gene="yoaE"
                     /locus_tag="ACHKUN_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO6660153.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(22873..23031)
                     /gene="yoaL"
                     /locus_tag="ACHKUN_001888"
     CDS             complement(22873..23031)
                     /gene="yoaL"
                     /locus_tag="ACHKUN_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO6660154.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            23348..24319
                     /gene="manX"
                     /locus_tag="ACHKUN_001889"
     CDS             23348..24319
                     /gene="manX"
                     /locus_tag="ACHKUN_001889"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO6660155.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            24382..25182
                     /gene="manY"
                     /locus_tag="ACHKUN_001890"
     CDS             24382..25182
                     /gene="manY"
                     /locus_tag="ACHKUN_001890"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO6660156.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25195..26046
                     /gene="manZ"
                     /locus_tag="ACHKUN_001891"
     CDS             25195..26046
                     /gene="manZ"
                     /locus_tag="ACHKUN_001891"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO6660157.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26101..26559
                     /gene="yobD"
                     /locus_tag="ACHKUN_001892"
     CDS             26101..26559
                     /gene="yobD"
                     /locus_tag="ACHKUN_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO6660158.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         6508 a         6877 c         6608 g         6616 t
ORIGIN      
        1 cctgtaatcg tttcacaggt caaatgatac tttggtctgt gaaaaaagaa accgatatcc
       61 ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc accagatacc
      121 gactccagat ttttgaccat gacgcgacag aaaaactacg cacttacttt tcaaatgtat
      181 gtgctgattt tgaagctgaa cttgttgaaa tggatggcga accagatcac gtccatttgt
      241 taattaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcgtat
      301 cgggtaggtt actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg
      361 ttttgtggag tcctggctat tttgccagta gctgcggagg tgcgccaata tccgtcatcc
      421 gccaatacat tgaacaacag caaacacctg gttagatgga aaaccgcgcc ttatatcccc
      481 gccctgaagg acggggtttt acggcgcacc ggataagaac gcacggtatt caccagatct
      541 tttatcactt cagccgccac ttctggcacc agcaaagtca tcggcgtctc tgtttcataa
      601 tcgacagaaa cgccattgct gttattggtg acggtcacgg tatacgttgc tttgcccatg
      661 attcatttcc cgttatgaat gactttccgt tgttgcgcac cttccatcag gacttcagga
      721 gccacgaaga agtcaatgtt gaaataagta tcgtcagtca tggcttcaat gttgtgccac
      781 ttttctggag ggaacaccgc aaactgcccc gcttcgataa ggatcacctg atcaggctct
      841 gcactgtgtt catcagcgta gccgagatat ttgaccgccc catgcataac ggaaaggcgt
      901 gggtaaaccc ccgggcgcgt tcctttatca agatgacgtt cgaatattcc ggcaggcgca
      961 gtttgtttat tccagaaagg cgttgagcgc gtatgaatat aattctgtgg gatttgcagc
     1021 atcctattct ctccttcggg gaatgcgctg aaaacggttt attccagccg tttcagggta
     1081 cgcctgataa tttgcatttt aaataccatt tattggttac tttttagcac catatcagag
     1141 aaaaatcagg gaggattaca agtggggagc ccgcgacaac gcgggctgaa tgtatcagga
     1201 ttgcagcgtc gccagtcggg cggcgaaacc cacgaacatc aaaccaatca gtgagttgcc
     1261 aactttagcc agtttctttt tggtacgtat gtactgcgtg acaaaagcac cagaaataat
     1321 caggaagctc aaatagcaga aactcaccag ttccagcgtc gtcgccagaa taaagaatga
     1381 aattcccgta tgtggggcat taacatcgat aaactgtacg aaaaacgaca catagaacaa
     1441 aatggctttc ggattagtca ggctcaaaat taacgcgcgt ttaaaaatgg ccccgtattg
     1501 gggctcatcg gatttggcct cgttattttt acccttcagg gtcgcgtaga gaattttact
     1561 ccccagatag agcaaataaa acgcaccaag ataacgtaca atgttgaata atatcggggt
     1621 ggtcttaatt aatgtcgcca ctccagccca tgccagaaac atcaatactg catcgccaat
     1681 aaatacaccg caggccgcaa gataaccgcc tttcataccg ctactgacgc tatttttgag
     1741 tacaaacagg gtatttggcc ctggcaccaa cacaataaaa atggccccaa ccagataggt
     1801 ccagtaattc agaaccccgt attcagcgaa cacattaacc tctttaatta tctttcgatc
     1861 atgcgcgatt aaaggtgaat atgctaacca atctgtagcg gcttagaaag gagaaaatca
     1921 ggttttaacc cgataccaac ccgataattg aatcattaat caggcatgct ccagtgaaaa
     1981 attcgggtag tgctcggcaa aatactggcg taaaaattct accgttatcc gcactttcgc
     2041 tgacgtcgcc agccttgaaa cataaacggc ccagacgttc gctggctggt aatattccgg
     2101 tagcacttgc actaaatgac cactggcaat gttttcgcta acatcccacc aggagcgcag
     2161 agcaatcccc tgcccatcga gacaccactg atggacaatt tctccatgat tagacgacag
     2221 tgggccggtg actttaatcg catggtggcc ttctttattg cgtaattgcc agacaccaaa
     2281 aggatgatcg cgctctttaa tgaccaggca cggcaatgcg gaaagatcgg ttaaatgttt
     2341 cggcgcgcca tgttgcgcaa taaattccgg cgacgcgcag agaatgcggt aattagtcgc
     2401 cagtttgcgg gcgattaaat taggggcgat gtcatcgcca atgcgaatat cgagatcaac
     2461 accttcattg accaaatcga ccaaccggtc ttccacatca aaacgtaatt ccagttgcgg
     2521 atacgcctta gccagcgctg ataacgccgg agccaccacc tgtcgtccaa aaccaaagct
     2581 gctgataata cgcagcatcc cctgcggcac ctgacgcacg tcagaaagtt cgtccatcat
     2641 ctgaccgaca tcctgcaaaa tccgctgcgc ccattcataa atccgctctc cttcttcggt
     2701 aatggtgacg cggcgggtgg tgcggtgtaa caacaccacg tttagcgttt gctccagcaa
     2761 ggcgatgcgc ttgctgacga acgccggtga aacgcccagt tcttccgcca cggcggcaaa
     2821 accagcccga cgagccacca gcataaagac gcgcaaatca ttcagcagcg gtaaattatt
     2881 catgattcgt gttttatgtt tcaccagtta cggggattaa ttccttttca gtcaattata
     2941 ggatggtgat gttgtcaatt ttgatggtca ggaagtgaga acccaatgat gaaaacgatg
     3001 cgtattgctg cgatcccggg agacgggatt ggcaaagaag tccttcctga agggattcgc
     3061 gtgttacagg ctgccgctga gcgctggggc ttcgccttga gttttgagca aatggagtgg
     3121 gcgagctgcg agtattacag ccatcacggt aaaatgatgc cggacgactg gcatgagcaa
     3181 cttagccgtt tcgacgccat ctattttggt gccgtcggct ggccggacac cgttcctgac
     3241 catatttcgt tgtggggttc gctgctgaaa tttcgtcgtg aattcgacca gtacgtcaac
     3301 ctgcgcccgg ttcgtctctt tcctggcgtt ccctgcccgc tggcgggaaa acagcctggc
     3361 gacatcgatt tttacgtggt cagggaaaac accgaaggcg aatattcctc gctcggcggt
     3421 agagtgaatg aaggtacaga gcatgaagtc gtcattcagg aatcggtatt tacgcgtcgt
     3481 ggtgtcgatc gcattttgcg ttatgccttc gaacttgcgc aaagccgccc acgtaagacg
     3541 ctaacttctg ccactaaatc aaacggttta gccatcagca tgccgtactg ggatgagcga
     3601 gtggaagcaa tggccgagaa ttacccggag atccgctggg acaagcagca tattgatatt
     3661 ctctgcgcgc gttttgtgat gcagccggaa cgcttcgatg tggtggtggc gtccaattta
     3721 tttggcgata tcctttccga tcttggcccg gcctgcaccg gcaccattgg cattgcccca
     3781 tccgccaacc tgaatccgga acgcactttc ccgtcgctct tcgagccagt ccacggttcc
     3841 gcgccggata tctacgggaa aaacattgct aaccctatcg ccacgatttg ggccggtgca
     3901 atgatgctcg attttctcgg caatggcgat gagcgtttcc agcaagcgca taacggtatt
     3961 ctggcagcaa ttgaagaagt gattgctcac gggccgaaaa cacctgatat gaaaggcaat
     4021 gccaccacgc cacaggttgc cgacgcgatt tgcaaaatta ttttgcgtta aggtcaaacc
     4081 agtttatttg aaccgcgtca ctgacgcggt ttttttattc gttctttgca gtaaataacc
     4141 tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg tattcttaat
     4201 tgtttaattt atgtaacatg caaataatca atttgttaaa cacaacatac aactcattac
     4261 cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccca ggagatgact
     4321 catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc tggtggccat
     4381 tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg cggccaacgc
     4441 aatatacact tttgttacgc gtacgttagg ttccgccgta caggtattgg ttttgctggc
     4501 aatgggactg gtgatttatt tagccaccag taaatacggc aatattcgtc ttggcgaagg
     4561 aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg gtttaggttc
     4621 ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcaaacgc ctggattaaa
     4681 tatcgcaccg cgttcacaac aggcactcga atttagcgtc ccttactcct tcttccactg
     4741 gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt atcactttca
     4801 tgtgcgtaaa aacaaaggtc tgagtctttc cggcattatt gccgcgatta ccggcgttcg
     4861 cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca ctgtcggcgc
     4921 actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt ccgcgctgac
     4981 cggtttaccc gataacttca ccgtgcaggc atttgtgatc ctgctttccg gcggcatttt
     5041 ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa cgtctgagca aaatggttgg
     5101 ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggcccaaccg aatttattac
     5161 caacagcatc atcaatgcca tcggtctgac cacgcaaaac ttcctgcaaa tgagcttatt
     5221 caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct actggctgtg
     5281 gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc gcggtcgtaa
     5341 gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct ggttcttctt
     5401 cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca acgtcccaca
     5461 ggtgctggaa acactgggcg gcgaaacagc tgtacagcaa gttctgatgt cgttgccagc
     5521 cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg cctcgcatat
     5581 ggatgcggtg gcctacacca tggctgcgac cagtacgcgt aatctccagg aaggtgacga
     5641 tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc cgctttccat
     5701 cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca cagcccttcc
     5761 cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga aacaggatta
     5821 cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg ttgaagccct
     5881 ggaaaaaacg ccagtgctcc ctgcgggaac cgtattcaaa ggcgacaact gagcgccatc
     5941 atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta ctacccgaaa
     6001 atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat accgatcaga
     6061 acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc gtcgctcaca
     6121 gcattgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc gaaagcatcg
     6181 tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt ccgcaccgtg
     6241 gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc ccgtatcacg
     6301 catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa aacgtcgcca
     6361 atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat gccggattcg
     6421 tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc ggtctgggcg
     6481 cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc cgctttacca
     6541 cccgcacgcc tgccaactgg aagaacattg tcgataacta tctcgagtgc tatcactgtg
     6601 gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg cacaccatgc
     6661 acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg tttaaatttg
     6721 aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg atgctgaacg
     6781 tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat tctgaaacta
     6841 ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag caaaaatcgc
     6901 tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt gaaagcgtac
     6961 agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc gacagtagcg
     7021 gtagtggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg caggtgttta
     7081 aggactaatg acatcggcgg cggtattttc cgccgctggg ctgatttttg atggagtaca
     7141 gcaatgtcag actatcaaat gtttgaagta caggtgagtc aggtggaacc gcttaccgaa
     7201 caggtgaaac gcttcacgct ggtggcaacc gatggcaaac cgctgcctgc gttcaccgga
     7261 ggaagtcaca tcattgtgca gatgagcgat ggtgataacc agtacagcaa tgcgtattca
     7321 ctactgagtt cgccgcatga cacctcttgt tatcagattg ccgttcggct ggaggaaaac
     7381 tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcgatcg gttaacgatt
     7441 tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct gtttatcgcg
     7501 ggcggtattg gtatcacccc tttcctgtcg cacatggcag agctgcaaca cagcgacgtc
     7561 gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg tgatgagcta
     7621 gtccagcatc cgcaggctga gaaagttcac ttgcatcact catcaaccgg aacacgactg
     7681 gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac ctgtggtccc
     7741 gaggcgctaa atgaagcggt aagaagtgaa gctgcgcgtc tggacatcgt cgccgatacg
     7801 ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcctttac cctggtgctt
     7861 gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca ggttattgaa
     7921 aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg aacctgcgaa
     7981 acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga tgaagagcgt
     8041 gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg cctggtgttg
     8101 gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg cttcggaaga
     8161 ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag ctcaccacgc
     8221 cagccggaaa tcagcgccgg caaattgttc tgcggtttca gtttccagtg ccagttcagc
     8281 agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg cgtttcgctc
     8341 acgtcagtaa tcagcgactt aatcgcttta aacactttac gataaccagg catgtccatc
     8401 aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt ttccaccagc
     8461 gccagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag gctgtccagt
     8521 tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc acgcacgaca
     8581 aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc taacagttgc
     8641 agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg ccaggcatct
     8701 tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc cagcgccgca
     8761 ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg taacagatac
     8821 cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc cagccagtcg
     8881 gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat ggaagcgaaa
     8941 ccccatgaca tcgggcgtcc gcagaaggca gcaaggattt gcgtgtcaat caagggttgt
     9001 ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc atggagaaat
     9061 tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt gatcccgaga
     9121 ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag ctgcgggtaa
     9181 taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc acggacggct
     9241 tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa attgttttct
     9301 ctttagtggg cgtcaaaaaa aacgccggat taaccggcgt ctgacgactg acttaacact
     9361 caggctttat tgtccacttt gccgcgcgct tcgtcacgta attctcgtcg caaaattttt
     9421 ccgacgttag atttcggtaa ctcatcacga aactccacca gcttcggtac tttgtagccc
     9481 gtgagctgac ggcggcaaaa ggtcaccagt gactcttcgg taagcgatgg atcttttttc
     9541 actacgaaga ttttcaccgc ttcaccactg gagccggaag gtacgccaac agccgcgact
     9601 tcctgtacgc caggatgctg catgacgaca tcttcaatct cgttgggata gacgttaaaa
     9661 ccggaaacca gaatcatgtc ttttttacga tcgacaatgc gcaggaaccc ttcttcatcc
     9721 atcaccgcga tgtcgccggt gtgtaaccag ccatttttga tgatctcatc tgtagcatcc
     9781 ggacgctgcc agtaacccag catcacctgc ggtcctttga cacaaagctc acccggttga
     9841 cccggtggta cttcattatc atcatcatcc accagtttgg cttccgtcga cggcaccggc
     9901 aaaccgatgc taccactatg ataatcaata tcatatgggt taacgctgac cagcggcgca
     9961 cactcggtaa ggccatagcc ttccagcaga tactgtcctg tcagtttcac ccaacgctct
    10021 gccaccactt gctgcactgg catccctccg cctgcggaaa gatgcagact ggagaaatcc
    10081 agctgctgga actctttatt gttcagcaac gcattgaaca aggtgttaac gcccgtgata
    10141 gcggtaaacg gatatttcgc taactctttt accaaccctg gaatatcgcg cgggttagtg
    10201 ataagcaggt tctgcccacc cagttcgata aacagcaggc agttaatggt cagggcaaaa
    10261 atgtgataca gcggcagcgc cgtcaccacc agctctttgc ccggatgcaa cagcggacca
    10321 taggtcgcgt taacctgttc caggttcgcc agcatattgc ggtgagtcag catcgcgcct
    10381 ttcgccacac cagtggtgcc gccggtgtat tgcagaaaag ctaaatcttc cggcaccagt
    10441 tcgggtttga cgtactgcat ccggtagccg ttatgcagtg cgctacgaaa tgaaatggca
    10501 tctggcagat ggtatttcgg caccaaacgc ttgatgtatt taacaacgaa attgactacc
    10561 gtgccttttg ccgtagatag ctgatcgccc atacgggtca gaattacgtg ctgaacggcg
    10621 gttttatcaa ccactttttc cagtgtgtga gcaaagttag acacgataac aatcgccgat
    10681 gcgccgctat cgttaagctg atgctcaagc tcacgcgggg tatacaacgg gttaacgttt
    10741 acgacgatca tcccggcacg caaaatgcca aacagcgcca ccggatattg caataaatta
    10801 ggcatcatca acgcaacgcg atcgcctttc ttcagcccca acccttgttg caaataagcg
    10861 gcaaacgcgc gactgcgttc ttccagcttg cggaaggtca ttacctcccc catattcaca
    10921 aacgcaggtt gatcggcgta gcgcgcgacc gactgctcaa acatatctac cagagattga
    10981 taacggtcag ggttgatctc cgtcggaacg tccgcgggat aacggttaag ccaaaccttc
    11041 ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg caaccccaaa tgatatacat
    11101 gccgttaaca taatattaac tcatcatacc agcttgataa ttacccaacg aaaaggttgc
    11161 gaagcgcgtc actatttatt tttatcttta ccgtaagaat gcagaaacag cggaccagcc
    11221 gctgtttctt tttctttaaa aacaagcaat tattcagtta caactgtttg tactctcgcg
    11281 gggccgggat tataccagcc ccatccgccg tagccatagg gccagccacg accgccataa
    11341 aaccatggat caatcggctg aggcggcata atcacctgct gggttaaatg ccaacgtttg
    11401 taacccgtta cttgcatcac cataaattta tagggcgtat tgccgatttt gccgtcaacc
    11461 gcaccagtga ttggcccgac taccgtaaca agttgtccac ggaaatccac cgggtccaga
    11521 aaaccgttca catcggcata aatgcgaccg cgagaaggtt ctcccagcgt cggtctggct
    11581 ccgctgtcca gcggtacggt agcaatttcc aggcgggttt tcccttgctg gttttgtacc
    11641 gcaaccactt tgccaccaaa gcgtgcctcc tgaccaacgt acagctgcgg cgcactcatc
    11701 acccgaacta aatcttgttg cggcgtggga ctgctgcctt taatggcgtc cggcacagtg
    11761 acacaaccgc tcagcattag cgcaaacgta cctgccagta tgcctttgat aacattcttt
    11821 tgaaccgcca tgatgcgact cctttttttc tcagggcata ctcttaagat tcattctttg
    11881 cccggaagtt tcttccatgc gacgttgtta cgtaaataaa ccggttcggc atgttccacc
    11941 gccaccgttt taccctcggc aaacatctga cacgcaatcg gcagcatatc ttccgccgct
    12001 ggcagtaaaa cttctccgtc gcgcaaaacc agcccgctct ctttaccgag atccggccag
    12061 gcttgccagc ccgtccctac cgttacccat tcgccggaaa gctgttgcat tcgttcatga
    12121 acgagttcgg gtttgagtac ggcttcggtt tcttcaccgc gccagatacc gttttcatca
    12181 cgctgatatt cggcccagta aacttcgccc attcgcgcgt caatggctgc cagcacgcgg
    12241 gttgcgccgt ttttgcgcca cgccccttgc gccatcgtca ttagcgtgga gacgccaatc
    12301 atcggtagct ctgcgccaag tgccagccct tgcgcaatgc caataccaat gcgcacgccg
    12361 gtaaagctac cggggccgcg cccgtaaacc agagcgttaa tatcagtcag ggaagttccg
    12421 ctggtggtca ggatatcctg caccatcggt aagattcgtt gagtatgttc acgagggcaa
    12481 agctcaaaat gagcgttgac agtaccgtcg ttccacaggg cgacagagca ggcctctgtc
    12541 gcggtatcga tagccagaat tcgcatgggt cttcgtgctt agatcaataa aaaggcgcgc
    12601 atcataccat actccgtaac aaattacctg gaggatggta tcgcaaggaa acgaaccgca
    12661 cgggcaatgt cacgagtgcg tggcgcgggc ggcagactgg cgagaaacgt cgcgccgtaa
    12721 ggacgcatca ccagccgatt gtcacaaatc accagcacgc cacgatcgtc ggcgtcgcga
    12781 atcagtcgcc ctaccccctg tttgagagta atgacggcat ctggtagttg cacttcatcg
    12841 aacgggtcgc caccgcgcaa acgacaatct tccatgcgcg cttttaacag tggatcatcc
    12901 ggcgaggtaa acggcaactt gtcgataatc accaatgaca atgtatcgcc acgcacgtcc
    12961 accccttccc agaaactact ggttgccaca agaagcgcgt taccggcgct gacaaattgc
    13021 tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa cgggaagcgt catggtagcg
    13081 cggaactgct cggccagatc acgcatcatg gcgtgcgagg tacaaagcat aaaacaacga
    13141 ccgttgttag cttcgatgat cggtcgcagc attgccgcta actggcgagc agaacctggc
    13201 tggttggttt gcggcagatt gcgcggcaca cagagtaacg cctggcggct gtaatcaaac
    13261 gggcttggca atagcaacga ctccgcctgt tctatcccaa gccgcgaggt gaaatgatgc
    13321 agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc agctaccagg cttttgcgcc
    13381 attaactctt tgaatttatc cgccacgctg agcggcgtga gagccagagt aaaatggcgc
    13441 gaagtgcatt cgtaccagta gctgtagccc ggctgattga tctcttttag ccgcttcagc
    13501 cgtgtgcgat acaacgtagc gcgctcaaat gccgcatcca gcaaggcgga acgccccagt
    13561 gacagtttcg ccacgtcata acaaagttcc agggtgtcat cgagcagtaa aaatgcccgc
    13621 tgaatttgcg gattagctaa cagctcacgc aggttaccgc gataacctgg ctcaccgagt
    13681 tgcagacgaa aatcctgcgc actctgggca agacgatcag cgcacttttg taactgctgg
    13741 gtgtctttta attcggtgcg gtaagcgatg gtgatgtctt ttgccaggtc gagcagttgt
    13801 cgactggaga gtgactgacc aaaatactgg ctggcaatgt ccggtagctg gtgggcttcg
    13861 tcgaagatca tgacgtccgc ttccgggatc agttcgccaa atccactctc tttaaccacc
    13921 atatccgcca gaaagagatg atggtttacc accaccacat cggcgtccat cgctttttta
    13981 cgtgctttga ccacaaagca atctttatac atcgggcagt cgctgccaag acagttgtcg
    14041 ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg ccacgctgac gcaggtgctg
    14101 atatcaccat cgactgtttg attagaccag gagcgcagca ggatcacatc gcttaagatt
    14161 tgtaccggca gatcgccccc cgccagcgcc tgctgttcga gacgctcgag gcagaggtag
    14221 tttgagcgcc ctttcagcag cgccacgttg cccgtatatt tcaatgcctt tgagactgtt
    14281 ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg agccggtcga gataatgact
    14341 ttctttttcg cccgcagcgc aggagccagg taagcgtagg ttttgcccgt accggttcct
    14401 gcttccacca ccagcggctg gcctttttct atcgcctggg tgacggctac cgccatctgt
    14461 cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg ccagctgacc gtctggtgca
    14521 aaatcgtccg tcacactacc ccctgttgat ttgaacaggg attatgtcag gatgagggcg
    14581 ctttcgccag ttgaattggt gacggcgacc tcacattgtg gcagtctttg cagcacgaaa
    14641 atggaaggat aatgaggaca aaatgatgac tatcgttcgt atcgatgctg aagcccgctg
    14701 gtctgatgtg gtaatccaca acaacacgct ctactacact ggtgtaccgg aaaacctcga
    14761 cgccgatgcc tttgagcaaa ccgccaacac gctggcgcag attgatgccg tgctggaaaa
    14821 acagggcagc aataaatcga gcattctgga tgccaccatt ttcctggccg ataaaaacga
    14881 cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc gcgggtcatg cgccggtgcg
    14941 ctgcacggta caagcgggtt tgatgaaccc gaagtataaa gttgaaatta agattgtggc
    15001 tgcggtgtaa gctttatcga agcaaaataa gtcagacgat aatttatcga taatactggt
    15061 cggttttaca taaatcgacc agagattaag atattacctg ttgatgctcc tgctaattca
    15121 ttatttattt ttataatttc aattttatct ataaaactaa ctatataaat aaccataacc
    15181 atgccgttgt gttcgccctc tgactagcaa ctctggtttt ctttcttagc tgaataatct
    15241 ggttatagcg tgaggactga ccgttaaatt tctccatctt actggataac acaccatgag
    15301 ctgctacctt taaaacacct gaatgtcaac aggttaactc gtgccatatc gttataatcc
    15361 ctttgatatt agatgcaaat taaggtcata tatagtctta tcgataacat ggttaatttt
    15421 aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg tgccatggat
    15481 gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat
    15541 ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa
    15601 ggctggaacc ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat ggagacaggt
    15661 gaacgctctg tgattaaaaa tcctgaatac ttttcaacat atatgcagga agaactaaaa
    15721 gcactggtct gagttaaatt tatatcagca taaatgggtc aggacgcttt taatcacata
    15781 taaaaagcgt cctttttctc acaatcaaat gatcatcggc ttctataacg aaatcgatac
    15841 ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattcgctc gccggagtgg
    15901 ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctgctcat cccttgtgcc
    15961 atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag tgaaggtaaa
    16021 cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat gctacccggc
    16081 acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat tactttaccc
    16141 tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc gtgggcgatg
    16201 cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt ggtcgccgat
    16261 ccgatttgca ctttaaccac tttcggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc
    16321 acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg cgcagacatt
    16381 cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt gtttggctac
    16441 gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat cgttctgccg
    16501 gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg tcatacagtt
    16561 tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc
    16621 tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac ccgcgagcag
    16681 tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg ctatcaggtg
    16741 aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc attccttcag
    16801 cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca gggtgcaatt
    16861 ttaagccttt cgccagagcg gtttattctt tgtgataata gtgaaatcca gacccgcccg
    16921 attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca agcagaaaaa
    16981 ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat
    17041 gatatcggtc gtgttgccgt agccggttcg gtaaaagtac cagagctctt cgtggtggaa
    17101 cccttccctg ccgtgcatca tctggtcagc actataacgg cgcgactacc agaacagtta
    17161 cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg ggctccgaaa
    17221 gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc
    17281 agcattggct atttgagctt ttgcggcaac atggatacca gcattactat ccgcacgctg
    17341 actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc cgatagccag
    17401 gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg ccaactggag
    17461 aagtaagacg tggaataccg tagcctgacg cttgatgatt ttttatctcg ctttcaactt
    17521 ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt gttaatcccc
    17581 atcgtccgtc gaccgcaacc ggggttgttg ctgactcagc gttcaattca tctgcgtaaa
    17641 cacgctggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc atcagttatc
    17701 gccgccgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc cgttgaagtt
    17761 atcggcgtgc tgccgcccgt cgatagcgta actggctatc aggtaacccc agtggtcggc
    17821 attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa
    17881 atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat ctaccgccgt
    17941 ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg gggaatgacc
    18001 gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga aaccctgact atacttatct
    18061 ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat cgcggttttt
    18121 gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt acactatgcg
    18181 ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat cgtgattagt
    18241 ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt agggcctatg
    18301 aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga tagcgttact
    18361 cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca ccacaccgat
    18421 atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat cgacagtatt
    18481 cccggtttta ttcgcgacgt agaagagcgc gaacgtctgc tgctggcaca gggacggcat
    18541 gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct gccgctgcat
    18601 gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag caaaacttat
    18661 tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga tgctgccaac
    18721 gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa
    18781 accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca cagcaagaaa
    18841 gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg
    18901 atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg
    18961 cgccggatgt tagtttccag cgataaactg tctaacgatc cgatgaatgt cattgactgg
    19021 gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact
    19081 gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta tgaccacttt
    19141 attgaatcgg tcagcccgga catctatacc cgttacttta tggcagcggg cgcgattggt
    19201 gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg
    19261 ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa
    19321 caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac ctgcgacccg
    19381 gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg
    19441 attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc gctggataag
    19501 gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg cgaaacctca
    19561 cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc ccatctgcaa
    19621 cggatgggcg aatttatacc cgctttctcg tctgctgtaa tattccccac tacacttcca
    19681 ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca
    19741 acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt
    19801 tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg
    19861 ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc ttcccctaca
    19921 ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg cccatttgcc
    19981 attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg tgcaagacgg
    20041 cacactctat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg acattctggc
    20101 tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc tgattaaagg
    20161 cagtccggtt ttgattcaat ggacgcctgc agcgggcagt agcaatgctg gggtcatgga
    20221 gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc cgcaaatcag
    20281 tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc tggtagattc
    20341 ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaacgct ttccttttac
    20401 cattaacgtt aatggtccgg gggctacggc gctggcatgg cactatcttc caacacaatt
    20461 accgctggcg gtgctgctaa gtttactggt gggctacatc gcctggctgg cgaccgctta
    20521 ccggatgagc ttttcccgcg aaatcaatct gggcctggcg caacatgagt tcgaattgtt
    20581 ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg
    20641 ctggaataat ccgcgtcagg gctggatttc accggatgtg tttattccta tcgcggaaga
    20701 acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc gtcagcgcca
    20761 tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca gccattttcg
    20821 ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc cgattcagca
    20881 actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc ggattgcccg
    20941 cgagctacat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg gcaacagttc
    21001 attttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt catttaccgc
    21061 agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg cgctggggca
    21121 aagactgaat attgaactgg tggcggaggg cgtggaaacg caagaacagg cgaagtattt
    21181 gcgccgtcat ggcgtgcata ttttgcaagg gtatttgtac gcacagccga tgccgctacg
    21241 tgattttccc aaatggctgg cgggcagcca accgccgccc gcccggcata atggacatat
    21301 cacgcccatt atgccgttac gttaatgctt actcatcttc atcgtgcgcc ggttgctctt
    21361 taacaatgcg aaccagatca acacgataat cattggcttc aatgatggtg atatgcagtg
    21421 gccctacatc aatcacatcg cccacacggg gaatgtgacc atttgccgag atcacgaggc
    21481 ccgcgaccgt cgcgatatca tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca
    21541 aggcatgcaa atctgtaccg ccttttacca gccagccgtc accatcagta atgatttccg
    21601 gcgtttcgtc agcgtccggg aattcacccg caatggcttc cagcacatcc agcggcgtga
    21661 ccagaccttg taccacacca aactcgttgg tcacgataac aaagctcccg cgagcacgac
    21721 gcagcacgcc caacaggttg atcggatcga gggtttccgg gacgataatc gccggagacg
    21781 ccgaagcaat cgccgccaca tcaacgccct cttccagcgc caccagcagt tctttagcac
    21841 gtacaatacc gatgatttca tccagttcac cgcgacatac cgggaacaga ctgtgcggtg
    21901 aagagagcag ttgctcgcgg atttcatcga ccccgagatt agcgtcaacc cagcttattt
    21961 caccgcgcgg cgtcatgatc ccgcgcagag aacgcgacgc cagcgtcagt acgccgttaa
    22021 tcatgtaacg ttcttcttcg gcaaatgcac cttccgggat cggcatcggc atcgggttat
    22081 cggcatcgtg ctgaacattg gcctgacgtt tcccgcccat caaacgcagg atggcatcgg
    22141 cagtacgcgc tcgcagcggc aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct
    22201 ggttaaacac ttcgatgatg atcgagaagc caatcgcggc atacaggtaa cctttcggaa
    22261 tgtggaaacc gaaaccttct gccaccagac tcagaccaat cattaacagg aagctcagac
    22321 agagcaccac caccgtgggg tgctggttaa cgaatcgcgt cagcggtttg gatgccagca
    22381 acataaccgc catcgcaatc actaccgccg ccatcatcac cggcagatgg ttaaccatcc
    22441 ctactgcagt aattaccgca tccaacgaga agacggcgtc aaggatgaca atctgtgtga
    22501 cgaccaccca gaaactggcg tagcctttac cgtggccgga atcatgatcg cggttttcca
    22561 gccgttcatg cagttcggtt gttgctttga acagcaagaa tatccccccg aacaacataa
    22621 tcaggtcgcg tccggagaag gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga
    22681 ccatccatga aatcagcgac agcagcccca gacgcataat cagcgccagt gataacccca
    22741 gcaaacgcgc tttatcgcgt tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga
    22801 ccaggttatc gatacccagc acaatttcga gaacaacaag cgtgagtagc cccgcccaaa
    22861 ttgaggggtc cattaagaat tccatgacaa gctcctgctt aaggaatagc tattcgacgc
    22921 cagaaataat gcaggcgtaa cgacaaaatg caaacgaaag gtgcggcata gagagccaga
    22981 aaggtaggcg ttaaaaggcc tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc
    23041 tactgcccta tactccatgg ttgttaaacg ggagttaaac atatcagaga cgcctctgat
    23101 ttggcaaaga tttaccttcc tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa
    23161 tttctgctaa tcgaaagtta aattacggat cttcatcaca taaaataatt ttttcgatat
    23221 ctaaaataaa tcgcgaaacg caggggtttt tggttgtagc ccttatctga atcgattcga
    23281 ttgtggacga cgattcaaaa atacatctgg cacgttgagg tgttaacgat aataaaggag
    23341 gtagcaagtg accattgcta ttgttatagg cacacatggt tgggctgcag agcagttgct
    23401 taaaacggca gaaatgctgt taggcgagca ggaaaacgtc ggctggatcg atttcgttcc
    23461 aggtgaaaat gccgaaacgc tgattgaaaa gtacaacgct cagttggcaa aactcgacac
    23521 cactaaaggc gtgctgtttc tcgttgatac atggggaggc agcccgttca atgctgccag
    23581 ccgcattgtc gtcgacaaag agcattatga agtcattgca ggcgttaaca ttccaatgct
    23641 cgtggaaacg ttaatggccc gtgatgatga cccaagcttt gatgaactgg tggcactggc
    23701 agtagaaaca ggccgtgaag gcgtgaaagc tctgaaagcc aaaccggttg aaaaagccgc
    23761 gcaagcaccc gctgccgcag caccaaaagc ggctccaact ccggcaaaac caatggggcc
    23821 aaacgactac atggttattg gccttgcgcg tatcgacgac cgtctgattc acggtcaggt
    23881 cgccacccgc tggaccaaag aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt
    23941 ggctgcggat accgttcgta agacactgct cacccaggtt gcacctccgg gcgtaacagc
    24001 acacgtagtt gatgttgcca aaatgattcg cgtctacaac aacccgaaat atgctggcga
    24061 acgcgtaatg ctgttattta ccaacccaac agatgtagag cgtctcgttg aaggcggcgt
    24121 gaaaatcacc tctgttaacg tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa
    24181 taacgcggtt tcggttgatg aaaaagatat cgaggcgttc aagaaactga atgcgcgcgg
    24241 tattgagctg gaagtccgta aggtttccac cgatccgaaa ctgaaaatga tggatctgat
    24301 cagcaaaatc gataagtaac gtattgtgtt gattatcact cagttttcac acttaagtct
    24361 tacgtaaaca ggagaagtac aatggagatt accactcttc aaattgtgct ggtatttatc
    24421 gtagcctgta tcgcaggtat gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta
    24481 atcgcgtgta ccctggtggg tatcgttctt ggggatatga aaaccggtat tattatcggt
    24541 ggtacgctgg aaatgatcgc gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac
    24601 gccgctctgg cttctatcat ttctaccatt ctggttatcg caggtcatca gagcattggt
    24661 gcaggtatcg cactggcaat ccctctggcc gctgcgggcc aggtactgac catcatcgtt
    24721 cgtactatta ccgttgcttt ccagcacgct gcggataagg ctgctgataa cggcaacctg
    24781 acagcgattt cctggatcca cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt
    24841 ccggccgtca tcgttgcgct gtctgttggt accagcgaag tacagaacat gctgaatgcg
    24901 attccggaag tggtgaccaa tggtctgaat atcgccggtg gcatgatcgt ggtggttggt
    24961 tatgcgatgg ttatcaacat gatgcgtgct ggctacctga tgccgttctt ctacctcggc
    25021 ttcgtaaccg cagcattcac caactttaac ctggttgctc tgggtgtgat tggtactgtt
    25081 atggcagtgc tctacatcca acttagcccg aaatacaacc gcgtagccgg tgcgcctgct
    25141 caggcagctg gtaacaacga tctcgataac gaactggact aacaggtgag cgaaatggtt
    25201 gatacaactc aaactaccac cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc
    25261 ctgcgttcta acctcttcca gggttcatgg aacttcgaac gtatgcaggc actgggtttc
    25321 tgcttctcta tggtaccggc aattcgtcgc ctctaccctg agaacaacga agctcgtaaa
    25381 caagctattc gccgtcacct ggagttcttt aacacccagc cgttcgtggc tgcgccgatt
    25441 ctcggcgtaa ccctggcgct ggaagaacag cgtgctaatg gcgcagagat cgacgacggt
    25501 gctatcaacg gtatcaaagt cggtttgatg gggccgctgg ctggtgtagg cgacccgatc
    25561 ttctggggaa ccgtacgtcc ggtatttgca gcactgggtg ccggtatcgc gatgagcggc
    25621 agcctgttag gtccgctgct gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt
    25681 tactacggcg tagcgtatgg ttactccaaa ggtatcgata tcgttaaaga tatgggtggt
    25741 ggcttcctgc aaaaactgac ggaaggggcg tctatcctcg gcctgtttgt catgggggca
    25801 ttggttaaca agtggacaca tgtcaacatc ccgctggttg tctctcgcat tactgaccag
    25861 acgggcaaag aacacgttac tactgtccag actattctgg accagttaat gccaggcctg
    25921 gtaccactgc tgctgacctt tgcttgtatg tggctactgc gcaaaaaagt taacccgctg
    25981 tggatcatcg ttggcttctt cgtcatcggt atcgctggtt acgcttgcgg cctgctggga
    26041 ctgtaagact gttgtacact accggggcct tttggccccg tttttttatc tggaggatta
    26101 atgacaatca cggacctggt actgattctt ttcatcgcag cactcctggc cttcgcgatc
    26161 tacgatcagt tcatcatgcc ccgccgtaac ggccccaccc tgctggcaat tcctttgctc
    26221 cggcgtggtc gcatcgatag cgttatcttc gtcggattga ttgtcattct tatctataac
    26281 aacgtcacga atcatggtgc gttaataacg acatggttat taagcgcact ggctctgatg
    26341 ggtttttata tattctggat ccgcgttccg aagatcatct ttaaacaaaa aggttttttc
    26401 ttcgccaatg tctggattga atatagccga atcaaagcga tgaacttgtc ggaagatggc
    26461 gtgttggtga tgcaattaga acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat
    26521 ctggaaaaaa tttataagct tctcgtttca actcaataag ttatgaattt agccaaagct
    26581 atgtttagtg tatttttaat aatcagaca
//
LOCUS       DAZFRJ010000050        26535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000050 DAZFRJ010000000
VERSION     DAZFRJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.050"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..961
                     /gene="xapR"
                     /locus_tag="ACHKUN_001893"
     CDS             77..961
                     /gene="xapR"
                     /locus_tag="ACHKUN_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO6660159.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(1070..1195)
                     /locus_tag="ACHKUN_001894"
     ncRNA           complement(1070..1195)
                     /ncRNA_class="other"
                     /locus_tag="ACHKUN_001894"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1227..1302)
                     /locus_tag="ACHKUN_001895"
     tRNA            complement(1227..1302)
                     /locus_tag="ACHKUN_001895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Lys,seq:ttt)
     gene            complement(1307..1382)
                     /locus_tag="ACHKUN_001896"
     tRNA            complement(1307..1382)
                     /locus_tag="ACHKUN_001896"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1347..1349),aa:Val,seq:tac)
     gene            complement(1429..1504)
                     /locus_tag="ACHKUN_001897"
     tRNA            complement(1429..1504)
                     /locus_tag="ACHKUN_001897"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Val,seq:tac)
     gene            complement(1549..1624)
                     /locus_tag="ACHKUN_001898"
     tRNA            complement(1549..1624)
                     /locus_tag="ACHKUN_001898"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1589..1591),aa:Val,seq:tac)
     gene            1883..3298
                     /gene="gltX"
                     /locus_tag="ACHKUN_001899"
     CDS             1883..3298
                     /gene="gltX"
                     /locus_tag="ACHKUN_001899"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO6660160.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(3350..3742)
                     /gene="yfeD"
                     /locus_tag="ACHKUN_001900"
     CDS             complement(3350..3742)
                     /gene="yfeD"
                     /locus_tag="ACHKUN_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6660161.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3744..4103)
                     /gene="yfeC"
                     /locus_tag="ACHKUN_001901"
     CDS             complement(3744..4103)
                     /gene="yfeC"
                     /locus_tag="ACHKUN_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6660162.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4324..4399
                     /locus_tag="ACHKUN_001902"
     tRNA            4324..4399
                     /locus_tag="ACHKUN_001902"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4357..4359,aa:Ala,seq:ggc)
     gene            4439..4514
                     /locus_tag="ACHKUN_001903"
     tRNA            4439..4514
                     /locus_tag="ACHKUN_001903"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4472..4474,aa:Ala,seq:ggc)
     gene            4722..6911
                     /gene="pdeA"
                     /locus_tag="ACHKUN_001904"
     CDS             4722..6911
                     /gene="pdeA"
                     /locus_tag="ACHKUN_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6660163.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(6961..8163)
                     /gene="nupC"
                     /locus_tag="ACHKUN_001905"
     CDS             complement(6961..8163)
                     /gene="nupC"
                     /locus_tag="ACHKUN_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO6660164.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            8499..9737
                     /gene="mntH"
                     /locus_tag="ACHKUN_001906"
     CDS             8499..9737
                     /gene="mntH"
                     /locus_tag="ACHKUN_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO6660165.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(9878..10204)
                     /gene="ypeC"
                     /locus_tag="ACHKUN_001907"
     CDS             complement(9878..10204)
                     /gene="ypeC"
                     /locus_tag="ACHKUN_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO6660166.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(10319..11575)
                     /gene="yfeO"
                     /locus_tag="ACHKUN_001908"
     CDS             complement(10319..11575)
                     /gene="yfeO"
                     /locus_tag="ACHKUN_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO6660167.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11779..12744
                     /gene="glk"
                     /locus_tag="ACHKUN_001909"
     CDS             11779..12744
                     /gene="glk"
                     /locus_tag="ACHKUN_001909"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO6660168.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            12963..13289
                     /gene="fryB"
                     /locus_tag="ACHKUN_001910"
     CDS             12963..13289
                     /gene="fryB"
                     /locus_tag="ACHKUN_001910"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6660169.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            13311..14558
                     /gene="fryC"
                     /locus_tag="ACHKUN_001911"
     CDS             13311..14558
                     /gene="fryC"
                     /locus_tag="ACHKUN_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6660170.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            14573..15658
                     /gene="ypdF"
                     /locus_tag="ACHKUN_001912"
     CDS             14573..15658
                     /gene="ypdF"
                     /locus_tag="ACHKUN_001912"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6660171.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            15658..16695
                     /gene="ypdE"
                     /locus_tag="ACHKUN_001913"
     CDS             15658..16695
                     /gene="ypdE"
                     /locus_tag="ACHKUN_001913"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6660172.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            16720..19215
                     /gene="ptsP"
                     /locus_tag="ACHKUN_001914"
     CDS             16720..19215
                     /gene="ptsP"
                     /locus_tag="ACHKUN_001914"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6660173.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(19218..20075)
                     /gene="ypdC"
                     /locus_tag="ACHKUN_001915"
     CDS             complement(19218..20075)
                     /gene="ypdC"
                     /locus_tag="ACHKUN_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6660174.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(20088..20822)
                     /gene="ypdB"
                     /locus_tag="ACHKUN_001916"
     CDS             complement(20088..20822)
                     /gene="ypdB"
                     /locus_tag="ACHKUN_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO6660175.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(20837..22534)
                     /gene="ypdA"
                     /locus_tag="ACHKUN_001917"
     CDS             complement(20837..22534)
                     /gene="ypdA"
                     /locus_tag="ACHKUN_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO6660176.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            22911..24149
                     /gene="alaC"
                     /locus_tag="ACHKUN_001918"
     CDS             22911..24149
                     /gene="alaC"
                     /locus_tag="ACHKUN_001918"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO6660177.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(24214..24285)
                     /gene="ypdK"
                     /locus_tag="ACHKUN_001919"
     CDS             complement(24214..24285)
                     /gene="ypdK"
                     /locus_tag="ACHKUN_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO6660178.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(24641..25561)
                     /gene="lpxP"
                     /locus_tag="ACHKUN_001920"
     CDS             complement(24641..25561)
                     /gene="lpxP"
                     /locus_tag="ACHKUN_001920"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO6660179.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            25914..26156
                     /gene="yfdY"
                     /locus_tag="ACHKUN_001921"
     CDS             25914..26156
                     /gene="yfdY"
                     /locus_tag="ACHKUN_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO6660180.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(26233..26460)
                     /locus_tag="ACHKUN_001922"
     CDS             complement(26233..26460)
                     /locus_tag="ACHKUN_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660181.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
BASE COUNT         6375 a         6514 c         7022 g         6624 t
ORIGIN      
        1 gtcggatatc tggtggtgaa atactttatg ccatgataat ttaatacgat gtatttatta
       61 tatggagcac ttaattatgg aacgcgtata cagaacagat cttaagttgc tccgttattt
      121 tcttgccgta gcggaagagt tgcattttgg ccgcgcagca gcgcgtttaa atatgtctca
      181 gcctccgctc agtattcata ttaaagagct ggaaaatcaa ctcggcacgc agctttttat
      241 tcgccattcg cgcagcgtcg tactgacaca cgcgggcaaa atcttgatgg aagaatcgcg
      301 tcgattgctg gtgaatgcta ataatgtatt ggctcgggtt gaacaaatag gtcggggaga
      361 agcagggcgg attgaactcg gcgtagtggg aacggctatg tggggacgga tgcgcccggt
      421 tatgcggcga ttcctcaggg aaaatcctaa cgttgaagtt ctttttcgcg aaaagatgcc
      481 cgcgatgcaa atggccctgc tggaacgacg cgaactcgat gccgggatct ggcgaatggc
      541 gacagaacca ccgacaggtt ttaccagctt acggttgcat gaatcggcgt ttctggtggc
      601 gatgcctgaa gagcatcatc tctcatcatt ttccaccgtt ccgctggaag cgctacgtga
      661 cgagtatttt gttacaatgc cgcccgtgta cactgactgg gattttttgc agcgagtttg
      721 ccagcaggtg ggattttcac cggttgttat tcgcgaagtt aatgaaccgc aaacggtact
      781 cgccatggtc agtatgggta ttggtatcac attgatagcg gacagctacg cacaaatgaa
      841 ctggccaggt gtcattttcc gtccgctcaa acagcgcatc cctgcagatt tatatattgt
      901 ttatgaaaca cagcaggtga cgcccgcgat ggttaagctg ttggctgcat tgacacagta
      961 ggtgaggaga caggaattgc cggaaatagt cgtctgctaa gtgaagcgca tcaggcaatt
     1021 tcgcaaatcg cagatagcaa aaaagcgcct ttagggcgct tttttacatt ggtgggtcgt
     1081 gcaggatgac tcgcttcgct cgcccttcgg gccgtcgccg cgagcggctc cgttgtttcg
     1141 ctgcgctcaa ctcgaacctg cggcagcttc gaatctttga gtatcgcaga tagcaaaaaa
     1201 gcgcctttag ggcgcttttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa
     1261 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccgaagtggt gggtgatgac
     1321 gggatcgaac cgccgacccc ctccttgtaa gggaggtgct ctcccagctg agctaatcac
     1381 ccacttcggt acttcacatt tttacaagaa atttagctgg cgagaaagtg gtgggtgatg
     1441 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
     1501 acccacttct caatttcttg ctacacggcg gagactacat aaagtagttg gtgggtgatg
     1561 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
     1621 acccccgctg tgtggaatcg cattataggg agagttcaaa atgagtcaac gcattttcta
     1681 aagaaattgt tcgttcgtcg taaatttaag caagatgatc gcaaaacaga ccgtgttgcg
     1741 caatttgtca acgaaaacaa taatgcgtaa ggttgcaacc cgaactacat tgaggaatca
     1801 ggcgggagtg atagaatatc gcccactcaa tttttccagg atttgccggt tgtcggcatc
     1861 tttctaaacg taaggccatt tcatgaaaat caaaactcgc ttcgcgccaa gcccaacagg
     1921 ctatctgcac gttggcggcg cgcgtactgc tctttactcc tggctttttg cacgtaacca
     1981 cggcggtgag ttcgtgctgc gtattgaaga caccgatctt gagcgttcca cgccggaagc
     2041 tatcgaagcc attatggatg gcatgaactg gctgagcctg gagtgggatg aaggtccgta
     2101 ctaccagacc aaacgttttg atcgctacaa tgcggtgatc gatcagatgc tggaagaggg
     2161 cactgcttat aaatgctatt gctctaaaga gcgcctggaa gcgctgcgcg aagagcaaat
     2221 ggcgaaaggt gagaagccgc gttatgacgg tcgctgccgc cacagccatg agcatcatgc
     2281 tgatgatgaa ccgtgtgtcg tgcgttttgc taatccgcag gaaggttctg ttgtttttga
     2341 cgatcagatc cgtggtccga tcgagttcag caaccaggag ctggatgatc tgattatccg
     2401 ccgtaccgat ggttccccaa cctataactt ctgtgtggtt gtcgatgact gggatatgga
     2461 aatcacccac gttatccgtg gcgaagacca tatcaacaac acgccgcgcc agatcaacat
     2521 tcttaaggcc ctgaaagcgc cggtgccggt ttacgcgcac gtttctatga tcaatggcga
     2581 tgacggtaaa aaactgtcca aacgtcacgg ggcagtcagc gtaatgcagt atcgtgatga
     2641 cggttatttg ccagaagcac tgctgaacta tctggtgcgt ctgggctggt cccacggcga
     2701 tcaggaaatc ttcactcgtg aagagatgat caaatacttc actttgaatg ccgtcagcaa
     2761 atctgccagt gcgttcaaca ccgacaagct gctgtggctg aaccatcact acattaacgc
     2821 gctgccgccg gagtatgttg ctactcactt acagtggcac attgagcagg aaaatatcga
     2881 tacccgtaac ggcccgcagc tggctgatct ggtgaaactg ctgggcgaac gctgcaagac
     2941 gctgaaagag atggcacaga gctgccgtta tttctacgaa gattttgctg agttcgatgc
     3001 cgatgcggcg aaaaaacatc tgcgtccggt agcgcgtcag ccgctggaag tggttcgtga
     3061 caaactggcc gcgattactg actggaccgc tgaaaacgtt catcacgcta ttcaggcgac
     3121 ggcggatgag ctggaagtgg gtatgggtaa agttggtatg ccgctgcgtg tcgccgtaac
     3181 cggtgcgggg cagtctccag cactggatgt taccgttcac gcaattggta agacccgcag
     3241 tatcgagcgt atcaacaaag cgctggattt tattgctgaa cgcgaaaatc agcagtaatt
     3301 agcgtgtaaa aagataaacg gcaggagata atatcctgcc gttttttatt tatgctgatt
     3361 cgtcaatatc gagacgggcg aggaaattgc gaattccttc acgtgacaaa aattgcgcga
     3421 ctttttcctg ctcaatgtct gtcatattat ctatcgcgtt gatgatttgt cgatacgccg
     3481 gggcgcgtgc tccaggagcg tattcagcaa ttctctcttc agcttccatc aacattggtg
     3541 tgttatggaa ggctggcgtg ttctgaataa actcgcaaac ttgcgtatcg accagaataa
     3601 gtctggcacg accgcctttc acgccgggaa atttttccgt tttccagcct ttttctctga
     3661 tccaacggtt aacagtttgt ttagccacac cgaggcattc agccagttct tcggtggtca
     3721 ttttgttgcg taatcttttc atattatttg ctatcgcgaa tccctaagcg ttgtaacaat
     3781 ccgataatcc cttcccgcag aagcaaggat gtaaactgtt tttgttcaac tggcgtcatc
     3841 tctttcgcca gtgtcaccag caatacttca agcggcgcat caccagaaag ggcaggcgct
     3901 tctccctgac cttctggacg ttcagcattg cgaatatatt cacgaacctg ttcattgacg
     3961 tgaaccagtc gggctttgcc accctggacg ccaggttttg gtgacgttgt ccagccttcc
     4021 ttgcgtaccc atttattaat ggtctggcgg ctatagccgg tcagtctggc aagttcatct
     4081 ggtgtcatcc tctccttgaa cataacaatt ccctgaatgt atgtgggtct attagtagtt
     4141 cattttataa caccacttta caggaaaccg tgacatgaat aactactgaa tgcgagcggt
     4201 tttccaaaat tgttcatttg cctgattttt cagcgattga aactacacgt tgcaatttgc
     4261 cgttgacaca ttcgggcgga attcatatga tgccgcccgt caactcgaca agctttacgt
     4321 gacggggcta tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcggttcg
     4381 atcccgctta gctccaccaa attttgcacc cagcaaattt ggtacgtaaa cgcatcgtgg
     4441 ggctatagct cagctgggag agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc
     4501 gcttagctcc accaaatttc caaccctcgc tgcaaagcgg gggttttttg tctctgcttt
     4561 ttgccgcttt tgtaatacag tctacgtccg ggttagcgcc gcctggtgaa agcatcattg
     4621 gatgaaaaat cggcaacagg ctggcccctg tttgcttcgc gatgcgaata aacttattat
     4681 ttgtgtgcct gaaaaccccg atcagtgaga gtagtgtact catgtttgtg gagcataacc
     4741 tgataaaaaa tatcaagata ttcacactag cgtttacgct caccgtggta cttattcagc
     4801 tatcccgttt tatttcgcca cttgccatta tccattccag ttatatcttt ctggcgtgga
     4861 tgccactgtg cgtaatgctg tcaatcttgt ttatctttgg ctggcgcggt gtcgttcccg
     4921 ttttatgcgg gatgttttgc accaatctgt ggaactttca tctctctttt ttacagactg
     4981 cggtcatgct cggtagccag acgtttgtcg tgttgtgtgc ctgcgcaata ttacgctggc
     5041 agctggggac gcgttggcgt tatggattga ccagccgata tgtctggcaa cgtctgttct
     5101 ggcttggttt ggtgacgccg atcggcatca aatgcagcat gtatcttgtg gggagcttct
     5161 ttgatttccc gctaaagata tccacctttt tcggcgatgc ggatgccatt ttcacggtcg
     5221 ttgatttgct aagccttttc accgctgtgc tgatttacaa catgcttttc tactatctca
     5281 ctcgcatgat tgtaagtccc cactttgcgc agatattgtg gcgcagggat atcgctccgt
     5341 cgttgggcaa agagaaacgc gcatttacct taagctggct ggcagcgtta agcgtgctgc
     5401 tacttctgtt gtgcacacct tatgaaaacg actttattgc cggttacttg gtacccgttt
     5461 tcttcatcat ctttaccctc ggggtcggta agcttcgcta tccgttttta aatctcacct
     5521 gggctgtttc aacgctttgc ctgctgaatt acaaccagaa ctttttgcaa ggggtggaaa
     5581 ccgaatattc gctggcattt attcttgcgg tgctgatttc ctttagcgtt tgcctgctct
     5641 atatggtgcg catttatcat cgcagtgaat ggcttaatcg ccgctggcat ttgcaggcgc
     5701 tgacagatcc gttaacgctc ctacccaact ttcgtgcgtt ggaacaagcg ccggagcaag
     5761 aggcgggcaa gagtttttgc tgcctgcgca ttgataatct tgagtttatg agtcgtcatt
     5821 acggcttaat gatgcgcgtt cactgtatcc gctcaatttg ccgtacgctg ctgccgttga
     5881 tgcaggaaaa cgaaaagttg tatcaattgc cgggtagtga actgctgtta gtgctgagcg
     5941 ggccggaaac ggaagggcga ctccagcata tggttaacat cctgaatagt cggcaaattc
     6001 actggaacaa taccgggctg gatatgggct atggtgctgc ctgggggcgt tttgatggaa
     6061 atcaggaaac cctgcaaccc ttgttggggc agttaagctg gctggcggag caatcctgcg
     6121 cacatcatca tgtgctggcg ctggatagca gagaggagat ggtttccggg cagaccacta
     6181 aacaggtgct attgctgaat accattcgca cggcgttaga tcagggtgat ttgctgctct
     6241 acgcccagcc aattcgcaac aaagagggtg acggttatga tgagatcctc gcgcgactga
     6301 aatatgacgg cggcattatg accccggata agtttctgcc ccttattgct cagtttaacc
     6361 ttagcgcgcg ttttgatttg caagtgctgg aatccttgtt gaagtggctg gcaacacacc
     6421 cttgcgacaa aaaaggaccg cgcttttcag tcaatttaat gccgctcacg ctgctgcaaa
     6481 agaatattgc cgggcggatt attcgtctgt ttaagcgtta tcacatctcc ccgcaggcgg
     6541 tcattcttga gatcaccgag gagcaggcgt tttctaacgc agaaagcagc atgtacaaca
     6601 tcgagcagct gcataagttt ggtttccgga ttgcgattga tgactttggc accggatatg
     6661 ccaactacga acggttaaag cgtttgcagg ctgatatcat caaaattgat ggcgtctttg
     6721 tgaaagatat tgtcacgaat acgctggatg cgatgattgt gagatcaatt accgatctgg
     6781 cgaaagcgaa gtcattgagt gtggtcgcgg agtttgtcga gacgcaacag cagcaggcgc
     6841 tattgcataa gctcggggtg caatatctgc aagggtattt gattggtcgc ccgcagccat
     6901 tagctgatta acaggcgtaa aaaaaccggg gaattatccc cggctttttt atgtatggtc
     6961 ttacagcacc agtgctgcga ttgacgcaga cagcacactc accagggtag agccgtaaac
     7021 cagcttcaga ccgaagcgag aaaccacgtt accttgctct tcattcaggc ctttaactgc
     7081 acctgcgata atcccgattg aagagaagtt agcgaaggaa accaggaaca cagagatgat
     7141 gccttcagca cgcggagaga gcgtggaagc aattttctgc agatccatca tcgcaacgaa
     7201 ctcgttggaa accagtttgg tcgccatgat actgcccact tgcagtgctt cactggaagg
     7261 aacacccatc acccatgcaa tcggatagaa gatgtagccc aggatgccct ggaaggagat
     7321 gctgtagcca aaccagccag taacggtggc aaacagtgcg ttcagcgcgg cgatcagggc
     7381 gataaagccg atcagcatcg cggcaacgat aatggcaact ttgaaacctg ccagaatgta
     7441 ttcacccagc atttcgaaga agctctgacc ttcgtgcagg ttggacatct ggatgttttc
     7501 ttcactggca tcaacacggt aaggattgat cagcgacagc acgataaagg tgctgaacat
     7561 gttcagtacc agcgcagcaa cgacgtattt cggttccagc atggtcatgt atgcaccaac
     7621 gatggacatc gacacggtgg acattgccgt ggcagccatg gtgtacatac gattacggga
     7681 gattttgccg aggatatctt tataggcaat aaagttttca gactgaccca gaatcaggga
     7741 gctgacggcg ttaaaggatt ccagtttgcc catgccgttg actttggaga gcaggaaacc
     7801 aattgcgcgg atgatcaccg gcaacacgcg aatgtgctgg agaataccga tcagtgcaga
     7861 gataaagacg attgggcaca gcactttcag gaagaagaat gccaggcctt gatcattcat
     7921 gctaccaaag acgaagttag tcccttcgtt ggcaaatccg agcagttttt cgaacatttc
     7981 ggagaagcct ttcacgaagc ctaaaccaac gtcggagttc aggaagaacc acgccagtaa
     8041 cacttcgata acaagcagtt gaataacata acggatacga atttttttgc ggtcgctgct
     8101 taccagcagt gcgagaatcg caacaacggc aagtgccagt acaaaatgaa ggacgcggtc
     8161 catatttgct ccaaatatga ggcaggttaa atttccgtgc acattctatg taacatggtt
     8221 aaggaaaacg agatctgaca cacactataa tgacgtcctg tgacaagctt caaaaatagt
     8281 gatctgtcat acatttctgc tatgttgtgt atggaagctg aaagttatgt aaatgtgcta
     8341 acattaaaca taaccataac attcaagtca tcaatctttt cgtagtcata atcctggtct
     8401 atcaaagaaa tcaccacaac ccattcaaat gaatttgata atcattctcg tttggcatag
     8461 catgaaacat agcaaaggct atgttttaga ggcacaagat gacgaactat cgcgttgaga
     8521 gtagcagcgg acgggcggcg cgcaagatga ggctcgcatt aatgggacct gcgttcattg
     8581 cggcgattgg ttatatcgat cccggtaact ttgcgaccaa tattcaggcg ggtgctagct
     8641 tcggctatca gctactgtgg gttgtcgttt gggccaacct gatggcgatg ctgattcaga
     8701 tcctctctgc caaactaggg attgccaccg gtaaaaatct ggcggagcag attcgtgatc
     8761 actatccgcg tcccgtagtg tggttctatt gggttcaggc agaaattatt gcgatggcaa
     8821 ccgacctggc ggaatttatt ggtgcggcga tcggttttaa actcattctt ggtgtttcgt
     8881 tgttgcaggg cgcggtgctg acggggatcg cgactttcct gattttaatg ctgcaacgtc
     8941 gcgggcaaaa accgctggag aaagtgattg gcgggttact gttgtttgtt gccgcggctt
     9001 acattgtcga gttgattttc tcccagccta acctggcgca gctgggtaaa ggaatggtga
     9061 tcccgagttt acctacttcg gaggcggtct tcctggcagc aggcgtgtta ggggcgacga
     9121 ttatgccgca tgtgatttat ttgcactctt cgctcactca gcatttacat ggcggttcgc
     9181 gtcaacaacg ttattccgcc accaaatggg atgtggctat cgccatgacg attgccggtt
     9241 ttgtcaatct ggcgatgatg gctacagctg cggcggcgtt ccacttttct ggtcatactg
     9301 gtgttgccga tcttgatgag gcttatctga cgctgcaacc gttgttaagc catgctgcgg
     9361 caacggtctt tggattaagc ctggttgctg ccggactgtc ctcaacggtg gtggggacac
     9421 tggcagggca ggtggtgatg caggggttca ttcgcttcca tatcccgctg tgggtgcgtc
     9481 gtacagtcac catgctgccg tcatttattg tcattctgat gggattagat ccgacacgga
     9541 ttctggttat gagtcaggtg ctgttaagtt ttggtatcgc cctggcgctg gttccactgc
     9601 tgattttcac cagtgacagc aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac
     9661 agacaggctg ggtgattgta gtgctggtcg tggcgctaaa tatctggttg ttggtgggga
     9721 cggcgctggg attgtagttg aatgagcgtc gcatctggca ctattggcgg atgcggcgta
     9781 aacgccttat ccgccctacg cgttaaagag ccggtttgta ggcctgataa gacgcgctag
     9841 cgtcgcatca ggcattgtgc tccattggag gacgcgttta atggcggtgc ccatgtccgc
     9901 ggccttttcc gcgatgatcg tcacgatcgc gccagccttc acgatagcca cgctcatatg
     9961 ctttacgctt atcccagcca cgatgatagc cattatcatg acgccaccag cggtttttgc
    10021 gccattcata attacgatgc cagtaatcac gatctcgcca gtgaccaccg tcccagtaat
    10081 taccgtaatg atcgcgatcg ccaatttgta atttgatcga tggcagtagg gtgatttcac
    10141 ctgcatttgc tgcaagcggg gtaaatgcca tcagggcggc cgccagaaac agtgacctga
    10201 acattgttat tctccttcac gatcgaagcc gtcagcggcc tgttaacgca atattacggg
    10261 gaggtaaagc cccgtatcat cgccataact cttaaatcat aaagaagagc attttttgct
    10321 aaacattatc gtggggtggc tgttgcttcg gacgattgac catcatcatc ggcttacctg
    10381 ctaataacag ccatgccgga agcatgacga tacagagcag cggtagcaat gtggtattgg
    10441 gtacaacgac tgccgccata aaaagactta accagccatc gcgtgttacc accagcacga
    10501 tgccgagaat agcgcaggaa acggttattg ccgctggaac ggcgggaacg tgctcatgca
    10561 gcatcaaccc taatgccacg ccgacaaaca ctgccgggaa gattcgccca ccgcgaaagc
    10621 cactggcggc agcaacgacc agggcggcaa gtttaattac cgccagcaaa aagtaatcgc
    10681 tggtgctgaa agcctgattt gccaccatct gctgcatctc atccagccct ttaaacagcg
    10741 aaaccggccc accaataact cccagaatac cgagaataaa tccgccaata cctagcacga
    10801 gcaccggatt tttcatttga tgcatcatcg cgtgcaaccg tggtaagcac catacggcga
    10861 ccattcctgc tgcgatggca atcgccgcga caattgcacc gctgagaata tcggtcattt
    10921 ccatctgtcc gtaatgcgca atgggcagtg aaaaatgggg atggaaaaat aatccggtag
    10981 taagtgcacc agctgctgcc gccattaatg gcgcaaaaag acgatcccat agcggaactt
    11041 cactactgcc atttaaggtt tgcgaaaata tcaacgccgc cgcaacaggt gtaccaaaca
    11101 gcgcgccgat ggttccggca gaggctaaaa tagtccactc cattcggttg acgcgcggta
    11161 acagacgagc gccaatggca accgcaaggg cgatattgac ggtcatgatc ggatgttccg
    11221 gccccaggct gacgccgcca gcaagaccga gaattaatgc tacgataagt cctggtagcg
    11281 cagagggcgg aactggtgcg ccgatcagcg gttcacaggc ggggtctggt ccggcatgac
    11341 cctggctgaa acggataacc aaccccaccg caatacccgt tagcgttaat acaccgatga
    11401 tccagagagg tgaatcctgg gctatcccca gagttcccgg cagtcgttgc cagagcaaat
    11461 tctgtaatac cgaggcgatt ttcatcacca caatcagaat aagactggac gcaatcccaa
    11521 ttgccacggc ggggagcgat aataacaaca tggttctggc tcgcggatgg agcatgttct
    11581 attccttatg cggggtcaga tacttagttt gcccagcttg caaaaaggca tcgctgcaat
    11641 tggtgctgaa acgataaagt aaatgtgtga cccagatcga tatttacagg gagcctgcct
    11701 ttccggcgtt gttgttatgc ccccaggtat ttacagtgtg agaaagaatt attttgactt
    11761 tagcggagca gttgaagaat gacaaagtat gcattagtcg gtgatgtggg cggcaccaac
    11821 gcacgtcttg ctctgtgtga tattgccagt ggtgaaatct cgcaggctaa gacctattca
    11881 gggcttgatt accccagcct cgaagcggtc attcgcgttt atcttgaaga acataaggtc
    11941 gaggtgaaag acggctgtat tgccatcgct tgcccaatta ccggtgactg ggtggcaatg
    12001 accaaccata cctgggcgtt ctcaattgcc gaaatgaaaa agaatctcgg ttttagccat
    12061 ctggaaatta ttaacgattt taccgctgta tcgatggcga tcccgatgct gaaaaaagag
    12121 catctgattc agtttggtgg cgcagaaccg gtcgaaggta aacctattgc ggtttacggt
    12181 gccggaacag ggcttggggt tgcgcatctg gtccatgtcg ataagcgttg ggtaagcttg
    12241 ccgggcgaag gcggtcacgt tgattttgcg ccgaatagtg aagaagaggc cattatcctc
    12301 gaaatactgc gtgcggaaat tggccatgtt tcggcggaac gcgtgctttc tggccctggg
    12361 ctggtgaatt tgtatcgcgc aattgtgaaa gctgacaacc gcctgccaga aaatctcaag
    12421 ccaaaagata ttaccgaacg tgcgctggct gacagctgca ccgattgccg ccgcgcgttg
    12481 tcgctgtttt gcgtcattat gggccgtttt ggcggcaatc tggcgctcaa tctcgggaca
    12541 tttggcggcg tgtttattgc gggcggtatc gtgccgcgct tccttgagtt cttcaaagcc
    12601 tctggtttcc gtgccgcatt tgaagataaa gggcgcttta aagaatatgt ctatgatatt
    12661 ccggtgtatc tcatcgtcca tgacaatccg ggtcttctcg gttccggtgc acatttacgc
    12721 cagaccttag gtcacattct gtaaatcctt cctgttatat cgggaggtaa ctctcccgat
    12781 aatcttttaa atcatacagt taattcaatt tttctttgtg tcccctcaca agaccgacct
    12841 gcgtcacact tccgtacagc gggattaatt ctccagtaaa tgcattattt gtctggtaac
    12901 ggcgatttgt tttgcacgtt cataatttca ctcgtcaact atcactgaat gaggaattaa
    12961 cgatgagtaa gaaactaatt gccttatgtg cctgcccaat gggcctggct cacactttta
    13021 tggccgctca ggcgctggaa gaagcggcgg tagaagccgg ttatgaagtg aaaattgaaa
    13081 cccagggcgc ggacggtatc cagaatcgcc tgacggcgca ggatatcgcc gaagcaacca
    13141 tcatcatcca ctccgtggca gttaccccgg aagataatga gcgtttcgaa tcacgcgacg
    13201 tttatgaaat cactttgcag gacgcaatta aaaacgctgc gggcatcatc aaagaaatcg
    13261 aagagatgat tgcctctgaa caacagtaat attcgacagg gataattgtt atggccatta
    13321 aaaaacgcag tgcaaccgtt gtgcctggcg catccggtgc ggcagcggca gttaagaatc
    13381 cgcaggcctc taaaagcagc ttctggggtg aactcccgca gcatgtgatg tcagggattt
    13441 cacgcatggt gccgacctta attatgggcg gtgtgatcct cgctttcagc cagctgattg
    13501 cttatagctg gcttaaaatt cctgctgaga tcggcatcat ggatgctctt aatagcggga
    13561 aattctccgg tttcgacctc tccttactga aatttgcctg gctgtcgcag tccttcggcg
    13621 gcgtgttgtt tggttttgcc attccgatgt ttgctgcttt tgtggcgaac tctatcggtg
    13681 gcaaactggc gttcccggct ggttttatcg gcggcttgat gtctacccag ccgacgcaac
    13741 tgctgaactt cgatcccagc actatgcaat gggcgacctc ttcgccggtg ccgtccacct
    13801 tcattggcgc gctgattatt tctatcgttg ccggttacct tgtgaaatgg atgaaccaga
    13861 aaatccagtt accggatttc ctgttagcgt tcaaaaccac atttttgcta ccgattcttt
    13921 ccgccatttt tgtcatgctg gcgatgtact acgtcatcac cccgtttggc ggctggatca
    13981 acggcggtat ccgtactgtg ctgactgccg caggtgagaa gggcgcgctg atgtacgcaa
    14041 tggggatcgc cgcggcgaca gcaatcgacc ttggtggccc gatcaacaaa gccgcaggtt
    14101 tcgttgcctt cagctttacc actgaccacg tattaccggt caccgctcgt tctatcgcta
    14161 tcgttattcc tccgattggt ctgggtctgg cgaccattat tgaccgtcgt ttaaccggca
    14221 aaggcctgtt caacgctcaa ctttatccgc agggtaagac cgcgatgttc cttgccttta
    14281 tggggattag tgagggtgcg attccgtttg cgctggaaag ccccatcacc gccattccgt
    14341 cgtatatggt cggcgcgatt gtcggctcaa ccgccgctgt ctggctgggc gcagtgcaat
    14401 ggttcccgga atccgctatc tgggcatggc cgctggtcac taacctgggc gtctatatgg
    14461 cggggatcgc gctgggggca gtcataactg cgctgatggt ggtgttcctg cgtctgatga
    14521 tgttccgtaa aggcaaattg ttaatcgata gcctgtaaga aggaaaatcc agatgacatt
    14581 actcgcttcg ctgcgcgact ggcttaaggc gcaacaactg gatgcagtgc ttctctcctc
    14641 acggcagaac aaacagccgc atctggggat ctccaccgga tcaggctatg tggttattag
    14701 ccgtgaaagt gcgcacattc tggtagattc gcgctatttc gttgaggtgg aagcccgtgc
    14761 gcaaggctac cagctgcatt tgcttgacgc gacgaacacg cttaccacta tcgtcaatca
    14821 aatcattgcc gatgagcagt tgcaaacgct cggttttgaa ggccagcagg tgagttggga
    14881 aaccgcgcac cgctggaagt ctgaactcaa tgcgaaactg gttagcgcca cgccggatgt
    14941 gctgcggcaa atcaaaacgc cagaggaggt ggagataatc cgcctcgcct gtgggattgc
    15001 cgatcgcgga gcggagcata ttcgccgctt tattcaggcg gggatgagcg agcgcgagat
    15061 agccgctgaa ctggagtggt ttatgcgcca gcagggcgca gaaaaagcct cttttgacac
    15121 cattgtcgcc agcggctggc gcggggcgct gccgcacggc aaagccagcg acaagattgt
    15181 tgcagcgggc gagtttgtca ctctcgattt tggtgcgctg tatcagggct actgctctga
    15241 tatgacgcgc accttgctgg tgaatggcga aggggtgagc gccgaatctc acccgctgtt
    15301 taacgtctac cagattgtcc tgcaggcaca gctcgcagca atctctgcaa ttcgccccgg
    15361 cgtgcgctgc cagcaggttg acgatgccgc gcgccaagtg attaccgagg caggttttag
    15421 ccactatttc ggtcataaca ccggtcatgc tatcggcatt gaagttcatg aagatccgcg
    15481 tttttcaccg cgggacacca cgacgctaca gccaggcatg ttactgaccg tggagccggg
    15541 gatttatttg ccagggcaag ggggcgtgcg catcgaagat gttgtgctgg ttaccccgca
    15601 aggcgcagaa gtgctctacg ccatgccgaa aacagtgttg ctcacgggag aggcataatg
    15661 gatttatcgc tattaaaagc gttgagcgag gcagatgcga tcgcctcctc ggaacaggaa
    15721 gtgcggcaga tcctgctgga agaagcggat cgcctgcaaa aagaagtgcg atttgatggt
    15781 ctgggatcgg tgctgatccg cctcaatgaa tcgacaggtc cgaaggtgat gatctgcgcc
    15841 catatggacg aagtgggatt tatggtgcgt agcatctccc gcgaaggggc aattgatgtg
    15901 ctgccggttg gcaacgtgcg catggctgcc cgccagctgc agccggtgcg catcaccacc
    15961 cgtgaagagt gcaaaattcc aggcctgctt gacggcgacc ggcaggggaa tgacgtcagc
    16021 gccatgcgcg tggacattgg tgcgcgctcg tatgacgaag tgatgcaggc gggaattcgt
    16081 ccaggcgatc gcgtcacctt tgatacaact tttcaggttc tccctcacca gcgggtgatg
    16141 gggaaagcct ttgatgatcg cctcggttgc tatctgctgg tgacgttact acgtgagctg
    16201 cacgatgccg aactgcctgc ggaggtgtgg ctggtggcaa gttccagcga agaggtggga
    16261 ttacgcggtg ggcaaaccgc tacccgcgcg gtgtcgccgg acgtcgccat tgtgctggat
    16321 accgcctgct gggcgaaaaa ctttgattat ggcgcggcta accatcgcca gattggtaac
    16381 ggcccgatgc tggtgttaag cgacaagtca ctgattgcgc cgccaaaact taccgcctgg
    16441 atcgaaaccg tggcggctga aattggcgta ccgttgcagg cagatatgtt cagcaacggc
    16501 ggcacggacg gcggagcggt gcacttaacc ggtactggcg tacccacagt ggtaatgggg
    16561 cctgccaccc gccacggaca ttgcgcggca tcgattgccg attgccgcga cattttgcag
    16621 atggagcaac ttttatctgc ccttattcaa cgtcttacgc gtgagacggt tgttcaactg
    16681 acggatttca gatgatctcc tgattaaccc ggagcggtta tgttaacgat tcaatttctc
    16741 tgtcctttgc ctaacggtct acatgctcgt ccggcgtggg aacttaaaga acagtgcagc
    16801 cagtggcaaa gcgaaatcac ctttttcaac catcgccaga acgcaaaggc cgatgctaaa
    16861 agctcgctgg cgttgattgg aactggcacc ttatttaatg acagttgcag cctgaacatt
    16921 agcggcagcg atgaagagca ggcgcggcgc gtgctggaag agtacatcct ggtgcgcttt
    16981 atcgacagcg acagcgttca gccgacgcag gcagaactga cggcgcatcc gctgccgcgt
    17041 tcattaagcc gccttaaccc ggatttactg tacggcaatg tgctggcaag cggcgtcggc
    17101 gtgggtacgc tgaccctgtt acaaagcgac agcctcgaca gttatcgggc aatccccgcc
    17161 agtgcgcaag attccacccg gctggagcac agcctggcaa cgcttgccga gcaactgaac
    17221 cagcaattgc gtgagcgtga cggcgaaagc aaaactatcc tcagcgccca tttgtcgctg
    17281 attcaggatg atgaattcgc gggcaatatc cgtcgtctga tgacagaaca gcatcaaggg
    17341 ctgggggcgg cgatcatcag caatatggag caggtttgcg ccaaactttc tgcttctgcc
    17401 agtgattatc tgcgcgaacg tgttagcgac attcgcgata ttagcgaaca gttgctgcat
    17461 atcacctggc cggaactgaa gccgcgcaac aatctggtgc tggaaaaacc gactattctg
    17521 gtagccgagg atttaacccc aagccagttt ttgagcctcg atttaaaaaa tcttgcaggc
    17581 atgattctgg agaaaactgg gcgcacctcg cataccctga ttctgacccg cgcctcggcg
    17641 atcccggtac tgagcggcct gccgctggat gcgattgccc gttatgccgg gcaaccggcg
    17701 gtgcttgacg cccagtgcgg cgtgctggcg attaacccga atgacgcggt gagcggttat
    17761 tatcaggtcg cacagacgct ggcggataaa cgccaaaaac aacaggcgca ggccgccgcg
    17821 cagctggcct attcccgtga taacaagcgt attgatattg cggcgaatat cggcaccgct
    17881 ctggaagcgc caggcgcgtt tgccaacggc gcggaaggcg ttggactgtt ccgtactgaa
    17941 atgctctata tggatcgcga cagcgcgccg gacgagcagg agcaatttga agcctaccag
    18001 caggtgctac tggcagcggg cgacaagccg attatcttcc gcacaatgga catcggtggc
    18061 gataaaagca ttccttatct gaatattccc caggaagaga acccgttcct cggctatcgc
    18121 gcggtacgta tttacccgga atttgctggc ctgttccgca ctcaactacg ggcgattttg
    18181 cgcgccgcca gttttggcaa cgcccagttg atgatcccga tggtccacag tctcgatcag
    18241 atcttatggg tgaagggcga gatccaaaaa gcgatcgttg agcttaagcg cgatggcctg
    18301 cgtcatgcag agacgattac gctggggatc atggtggaag tgccgtcggt gtgctacatc
    18361 atcgatcact tctgcgatga ggtcgatttc ttcagtatcg gctccaacga tatgacccag
    18421 tatctgtatg cggtcgatcg taataacccg cgcgtatcgc cgctgtataa cccgattacg
    18481 ccatcgttcc tgcgcatgtt gcagcagatt gttaccactg cgcatcagcg gggcaaatgg
    18541 gtaggcattt gcggtgaact gggcggtgaa agccgttatc tgccgctact gcttgggctg
    18601 ggcctggatg agctgagtat gagtagcccg cgtattccgg cggtgaaaag ccagcttcgt
    18661 caactggata gcgaggcgtg tcgggaactg gcgcgtcagg catgtgaatg ccgcagtgcg
    18721 caggaaattg aagcgttact caccgccttt acgccggaag aagacgttcg cccactgctg
    18781 gcgctggaga atatctttgt tgatcaggat tttagcaata aagagcaggc gatccagttc
    18841 ctgtgcggca acctcggcgt taacgggcgc actgaacatc cgtttgagct ggaagaagat
    18901 gtctggcagc gggaagagat tgtcaccacc ggcgttggtt ttggcgtagc gatcccgcac
    18961 accaaatctc agtggatccg tcattccagt atcagcattg cccggctggc gaaaccggtt
    19021 gactggcagt cagaaatggg cgaagtcgaa ctggtgatca tgctgacgct gggtgctaac
    19081 gaagggatga atcatgtgaa agtcttctcg cagctggcgc gtaaactggt gaataaaaac
    19141 ttccgccagt cgttgtttgc cgcgcaagat gcacaaagta tcctgacgct gctggaaaca
    19201 gaattaacct tctgacgtta gccctgaaaa cgggcgctgt actctcccgg cgtcagacca
    19261 aactgacgcc ggaaaacgcg acaaaaatag tcgctatccg gaaaaccgca acgctgtgcc
    19321 acttcatgaa ttgacagatg atatttctgc aaaatcatcc tcgccttcgc cattcgcacc
    19381 caacgcacat actcgataaa acgcatcgtt ccatgctgag caaacagttt tgacagatga
    19441 ttgggcgtga tattaaaaaa ctgcgccacg ctctcgcggg tgagcggctg ggcataatta
    19501 tcctgaaccc agttacaaat gctgtgatag agaaaatcgc cgcgcggttg cgaattaccc
    19561 ggcggcatat tcaccacgtt gcggcaaaga tgcagcaaac ttaacaccag tggctgaatg
    19621 atattctgct ccagcggcga acggctaaga tgggtgaggg cggtaagcat cgcttcacct
    19681 tcaccgcgtt gcaggctggg aagctggatc tggcgagcag gatgcaacaa cgacgtggtg
    19741 cggctatcgt aaaacgataa cccaagccag gtcggtgcga acaccaggct taacagcatc
    19801 accggtttgt tgttgatcgg taaattagcc gcgcgtgccg gaataaacag catttctcct
    19861 tccaccattc gctgctgctt tgcttccagc aggttgccgt attcgccgcg cagcacgata
    19921 tccagacggg gaaaatcaat acataaactg cccaccggta acgaggcagg ctggctggca
    19981 aaccagacgc gccccagtag ttgcgggtta agcaccaggc cgctgaacag atcggcgaaa
    20041 aattgttgat cggcgggcaa gcctggtgct ttcatgaaag ttcccgatta aagatgcatt
    20101 aactggcgaa attctttcac tttgctgcga ctgaccggca cttcaaaatc cagatctttc
    20161 agtcgcagaa tgtaggtgtt attaaaccac ggttcgattt cgcgtatttt gttcagattg
    20221 acacaaaatg agcgatggca gcggaaaaaa tgcgacggcg gcagtttgct gcaaaattcg
    20281 gtaatgttca tcggcattac gtaggattca cgccgcgtat agacaaaggt cattttctcg
    20341 tgcgcttcgg cgtaatagat atcgttaatt ggcgtgacga ttattcgctc atctttcacc
    20401 agattaatgg tgtcattctc acgcgttacc gtcgcggcag gcgtactgct ggtctgctgt
    20461 tgttgccagg ccgcttccag tttttgcagc atcccggtaa tacgtgactc ctgatacggt
    20521 ttgagaatgt agtcaaacgc ctccagttca aacgcttcta ccgcatgttc tttccacgcg
    20581 gtgatgaaca caataaacgg tttattggcg aactggctga tgttttgcgc cagcaacacg
    20641 ccatccagcg acggaatatt gatatccaga aaaatggcgt cgacgcggtt atgctgcaaa
    20701 aacttcaaca cgtccagacc gtcgtcaaag gtgccgacaa tctccatctg gctgtgctct
    20761 ttaattagcc agctcagttc ctgttgtgcc aggaattcgt cttcaacaat gatgactttc
    20821 acaatatcac tccggctcaa agcaataacg tagcctgtga ggcgactggg gtgcgttggt
    20881 taggaatgta aaacgcaatt tccgtccccg gctccaggcg gcggatatgc agcccctcgc
    20941 catacaataa cttcacgcga tgatggacat tcagcaggcc aattttattg cccggcattt
    21001 cattcgcttc gacccgctca atcacctttg gatcgatgcc gtgcccggta tctcgcaccg
    21061 caatgcgtac ccgatttccg cactctgcaa cgctgatggt gacaacgcct ttacctttgc
    21121 aaggctgaat accgtggaca atggcgttct ccaccaacgg ctggatcagc aggctgggaa
    21181 tgcagcaatt cacctcttca tcaatatcat agataaccgt cagcttgtca ccaaagcggg
    21241 cctgctcaat ggcaatatag tctttaattt gatacagctc ttttttgata tcgatttgct
    21301 catcgtcttt taattcaatg ttatagcgca gataacgcga cagattaaag atcaactggc
    21361 gagcggtatc cggattcaga cggattgacg atgaaatagc gttcagagcg ttaaacagaa
    21421 aatggggatt aattttgctt tgcagggcgc gaagctctgc cttatttgcc atttcacgta
    21481 gctgctcggc gcgtgaaacc tccagttgcg tggagatgat ttgcgacaga ccgaccgcca
    21541 tttcctgtaa tgacgaggtg atctgatgcg cgtggcagta gtaaattttc agcgttccgg
    21601 tcacgacccc tttctcccac aatgggatca ccagcatgga atgaatctct ggtgtgcggt
    21661 gggcttcatc attgttttta atgatgattt ttccgtaatt catcgcctga cgggtagtcg
    21721 ggctaatgaa gtcatcgcca ttctgatagt tatgttcacc cacgccaaca taggccagca
    21781 catgatcggt attagtaatc gccaccgcat cggcgtgaat gtcatcgcgg ataatttcgc
    21841 agaccttgcg taatgactcg ctattgacat ggcgaaacag cggtagcgtt ttgttggcga
    21901 tatccagcgc cagcttggcc tgccgcgcgg cgctggcctc tttttcgccc tcaacgcttt
    21961 gcaccagaag cacaataaag ccgatacaga cgctaccgag gatcattgga atgccgattt
    22021 tagagacgat atcgatcccc aacgcggtag ttggtgccca gacaattacc agaatcatgg
    22081 tcaacgtctc acacaacatg ccgccgagaa taccgacgcg ccagcgctgt gctttgggga
    22141 ttttcaggtt gatccagccc gatatacaac ccgccagaat gctggtgata aagcagggga
    22201 tcgccgtcac gccgccgata tcaattaaat accggtgaat acccgcaatc acgccggtaa
    22261 tgatgcctac ccacgggcca aacagaatcc cgccggacat caccgcgata atacgtacgt
    22321 tcaccagcga gccttctacg ggaacgccgg accaggtgct gaacagggcg aacagcgaga
    22381 aaatggcggt aacggcgagc aattcttttg gggagtgcgc cgacttgtgc aacagttcgc
    22441 gaaacaggcg gatacggatg agaaagaaca ggcagataag cattaacgct gcccgatcga
    22501 agaccgccag cagcatgttg aatatttcgt gcacgggtga ctctgaataa ggattaaacc
    22561 tctatgatag gtaacctgaa ggctgatgac cagcaggccg tttttgagga aaaaccataa
    22621 atttaaaagg ggattgaaag ataaatggtg tattagcaat gtatctcttg ataatgcact
    22681 tctggttggt aaatttattg aaattataat gttaataaaa ttggtggtaa ttttcttgtt
    22741 agcgtagaaa aatttcctgt ttttgaagtg gagttcctca tttaaagcca ttgataaaaa
    22801 tgttctaaaa cgctggacag acagaaatta atcaggctat tttcaaaaca gccaccattg
    22861 tggcagcgtc gctcggacgg tccgggcgct aacgttaatc tgaggaaatt atggctgaca
    22921 ctcgccctga acgtcgcttt acgcgcattg atcgtctccc gccctacgtt tttaacatca
    22981 ccgctgaact gaaaatggct gcgcgtcggc gcggcgaaga tattatcgat ttcagcatgg
    23041 gtaacccgga cggtgcgact ccgccgcata tcgtcgaaaa attatgtact gtggcccagc
    23101 gcccggacac gcatggttac tccacttcac gcggcattcc gcggttacgt cgcgccattt
    23161 cccgctggta tcaggatcgc tacgacgttg aaatcgaccc ggaatcagaa gccatcgtca
    23221 ctattggttc gaaagagggc ctggcgcatc tgatgctggc gacgctggat catggtgaca
    23281 cggtgctggt gccgaatcca agttacccga tccatattta tggcgcggtg attgccgggg
    23341 cgcaggtacg ctcagtgccg ctggtggaag gtgtcgattt cttcaacgaa ctggagcgcg
    23401 ccattcgtga aagttatccg aaaccgaaga tgatgatcct cggcttcccg tcgaatccaa
    23461 ccgcgcaatg cgtggagctg gagttcttcg aaaaggttgt ggcgctggcg aaacgctacg
    23521 atgtgctggt ggtccacgac ctggcctatg ccgatatcgt ctacgatggc tggaaagcgc
    23581 cgtcaatcat gcaggtaccg ggtgcacgcg atgtggcagt cgagttcttt acgctgtcga
    23641 aaagctacaa catggcgggc tggcgtatcg gctttatggt tggcaacaaa acgctggtca
    23701 gcgccctggc tcgtattaaa agctatcacg attacggcac ctttacgccg ttgcaggtgg
    23761 cggcgattgc ggcgctggag ggcgatcaac agtgcgtgcg cgacattgcc gaacagtaca
    23821 aacgccgccg tgatgtattg gttaaagggc tgcatgaagc gggctggatg gttgaaatgc
    23881 cgaaggcttc gatgtatgtc tgggcgaaaa tcccggaacc atatgcggcc atgggatcgc
    23941 tggaatttgc caagaagctc cttaacgaag cgaaggtttg tgtctcgccg gggattggct
    24001 ttggcgacta tggtgatacg catgttcgct ttgcactgat tgaaaaccgc gaccgtattc
    24061 gtcaggcgat tcgtgggatt aaagcgatgt tccgtgccga cggtttacta cccgccagca
    24121 gcaaacatat tcacgaaaac gcggaataaa caccgtcgtc agaaatgcaa acaggagcct
    24181 gatggctcct gtttttcatt gcacagtttg cttttagatc atcagggcaa aagtaccggc
    24241 ccaaacgatg accataaaag aaatgcccat aaagaaatat ttcactttac atctctccgc
    24301 gtaattactg ttacgcctga atgacttcat tacacctgat acagtgagtt gacaccgggc
    24361 aaagcgacct taaaagccct gtattttacc gtgggcatca ccccagaatt ctgagctaaa
    24421 ctgctcgaga cggcgctaat gtacgcctaa aactgaaacg agacaagaga cattttctta
    24481 tttactttta gtaacttaca agtgtctcat atcggcgaca gtttaatttc tgacggtaat
    24541 aattagatat tcagcgaagg aaaaaacctt aagaaaaaag ccataaaaac catgaggtta
    24601 ttatggccga tttgaggagg gaaagagtaa gagcagtttg ttaaatgtac aacgacgatt
    24661 ctcccaccgg gcgcgtttta aagcgacggt ggatccagag gtactgctcc ggtgcgcgca
    24721 tgatctcttt ctcgataatc ttgttcatat aggcagcggc ttgattttca tctgtcgggt
    24781 agccttccat ctctggggtg atgaacaaac gatatccgct gtaatccgct tttcttacca
    24841 tcgttacggt caacatggct gcgccagaga gacgggagag aacataggtg ccattggttg
    24901 tggcgacatt ttccaccgca aagaacggcg cgaaggagct gcctttacga ccataatcct
    24961 gatcgggagc aaaccatacc gcttcacctt tcttcagtgc accgacaatg ccgcgcagat
    25021 tatttctgcc gatcatcgct ttgttagagc gcatgcgccc acgggtctgc acccattcca
    25081 tcagctgatt attatgtgga cgataggtag ccatcattgg ttggcacagt cccatcacgc
    25141 ggccgcccag ttccagcgac ataaaatgga cgccgacaac cattacgccg cgattttgca
    25201 tttgtgcgcg tttaaggtta tccaaccctt caacatcaaa ccatttacgt acgcgactgt
    25261 cgggccagaa ccatgccatg ccggtttcta ccagcgccat gccgagtgaa cgaaagtttt
    25321 cggcaatcat cttctcgcgt tcttccgcag aatgctgcgg gaaacaaagt tccaggtttt
    25381 tacgggcgat agattcacga cgtttcagga atggtcgcgc cattgcgcca atacgcgtgc
    25441 cgagaaagca gagaacagga taaggaagct gtacccaaag ccagagtaca ccaagcccaa
    25501 accatgtgag ccagtagcga ggatgtagaa actcgcggga aaatttgcat tgtggaaaca
    25561 tagttacctt ctgtagatac gaattgttgt atcgcagaag attccatgtc gacagcggta
    25621 cactcaattg gttgggccat catgtacgac aatggttcct gtaaagagtg taaagactag
    25681 tcaggacata accacgagga gcatgaatgc ggtatctgta ctttatcact cgctgactgc
    25741 tttcagctac tgattattaa tcgtgcaaag taaatatttt tccgtacaca acagtcttgc
    25801 atttaacatg ctgttttcat tgatgcttag caaaaattgc cagaggttaa ggattgtctt
    25861 actattaaag tgactgaagt tacaggtttt ttagcgcggc aggaggttga gctatgattt
    25921 atttatggat gtttctcgcc ctgtgtattg tctgcgtaag cggctacatc ggtcaggtac
    25981 tgaacgtggt atcggcggtc tcttctttct tcggcatggt gatcctcgct gcactcattt
    26041 attacttcac catgtggtta actggcggta atgaactagt gacggggata tttatgtttc
    26101 ttgccccggc ttgtggcttg atgattcgct ttatggtggg gtatggcagg cgatagtggg
    26161 tcaggatggc tcatttgggc aggtgttcac atcagcatca gacatgattg ttcacctgcc
    26221 atgtgacaga cgctatcgca caggatgaat aagataattc gcctttgtcg gataagtagg
    26281 cgtgatttct tctgtgaggt ttaatggtgg cgcatcttct ctcatcttaa acaaaatcgc
    26341 ctgtgatggc tgaagttttg tagagtgtgt ggttgtcatc gctgatgttt tttcagtctc
    26401 tttatagctc ctctcactta caccaccaga aatggaaaat gcaaaaacat tgcatgccat
    26461 aatagagacc aataaaaata agctgaaaag tattaagttt actttcatag aaagttatcc
    26521 tgaaaatggt tcact
//
LOCUS       DAZFRJ010000051        26258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000051 DAZFRJ010000000
VERSION     DAZFRJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.051"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1145
                     /locus_tag="ACHKUN_001923"
     CDS             <1..1145
                     /locus_tag="ACHKUN_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6660182.1"
                     /translation="FIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARN
                     MAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSE
                     IFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDL
                     IYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSW
                     AAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIP
                     GIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPG
                     PLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1173..1484
                     /locus_tag="ACHKUN_001924"
     CDS             1173..1484
                     /locus_tag="ACHKUN_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO6660183.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1540..3213
                     /locus_tag="ACHKUN_001925"
     CDS             1540..3213
                     /locus_tag="ACHKUN_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6660184.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3238..4677
                     /locus_tag="ACHKUN_001926"
     CDS             3238..4677
                     /locus_tag="ACHKUN_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6660185.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4734..4952)
                     /gene="marB"
                     /locus_tag="ACHKUN_001927"
     CDS             complement(4734..4952)
                     /gene="marB"
                     /locus_tag="ACHKUN_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO6660186.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4984..5367)
                     /gene="marA"
                     /locus_tag="ACHKUN_001928"
     CDS             complement(4984..5367)
                     /gene="marA"
                     /locus_tag="ACHKUN_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO6660187.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5387..5821)
                     /gene="marR"
                     /locus_tag="ACHKUN_001929"
     CDS             complement(5387..5821)
                     /gene="marR"
                     /locus_tag="ACHKUN_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO6660188.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6033..6698
                     /gene="marC"
                     /locus_tag="ACHKUN_001930"
     CDS             6033..6698
                     /gene="marC"
                     /locus_tag="ACHKUN_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO6660189.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6723..7913)
                     /gene="ydeA"
                     /locus_tag="ACHKUN_001931"
     CDS             complement(6723..7913)
                     /gene="ydeA"
                     /locus_tag="ACHKUN_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO6660190.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8063..9177)
                     /gene="yneK"
                     /locus_tag="ACHKUN_001932"
                     /pseudo
     CDS             complement(8063..9177)
                     /gene="yneK"
                     /locus_tag="ACHKUN_001932"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(9255..10136)
                     /gene="yneJ"
                     /locus_tag="ACHKUN_001933"
     CDS             complement(9255..10136)
                     /gene="yneJ"
                     /locus_tag="ACHKUN_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6660191.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            10237..11625
                     /gene="sad"
                     /locus_tag="ACHKUN_001934"
     CDS             10237..11625
                     /gene="sad"
                     /locus_tag="ACHKUN_001934"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO6660192.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            11689..12615
                     /gene="glsB"
                     /locus_tag="ACHKUN_001935"
     CDS             11689..12615
                     /gene="glsB"
                     /locus_tag="ACHKUN_001935"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO6660193.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            12615..12974
                     /gene="yneG"
                     /locus_tag="ACHKUN_001936"
     CDS             12615..12974
                     /gene="yneG"
                     /locus_tag="ACHKUN_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO6660194.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            13113..14531
                     /locus_tag="ACHKUN_001937"
     CDS             13113..14531
                     /locus_tag="ACHKUN_001937"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6660195.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            14758..16209
                     /gene="uxaB"
                     /locus_tag="ACHKUN_001938"
     CDS             14758..16209
                     /gene="uxaB"
                     /locus_tag="ACHKUN_001938"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO6660196.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            16416..17330
                     /gene="yneE"
                     /locus_tag="ACHKUN_001939"
     CDS             16416..17330
                     /gene="yneE"
                     /locus_tag="ACHKUN_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO6660197.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(17334..17828)
                     /locus_tag="ACHKUN_001940"
                     /pseudo
     CDS             complement(17334..17828)
                     /locus_tag="ACHKUN_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(17827..18663)
                     /gene="lsrF"
                     /locus_tag="ACHKUN_001941"
                     /pseudo
     CDS             complement(17827..18663)
                     /gene="lsrF"
                     /locus_tag="ACHKUN_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            complement(18690..19712)
                     /gene="lsrB"
                     /locus_tag="ACHKUN_001942"
     CDS             complement(18690..19712)
                     /gene="lsrB"
                     /locus_tag="ACHKUN_001942"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO6660198.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            complement(19724..20716)
                     /gene="lsrD"
                     /locus_tag="ACHKUN_001943"
     CDS             complement(19724..20716)
                     /gene="lsrD"
                     /locus_tag="ACHKUN_001943"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO6660199.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            complement(20716..21744)
                     /gene="lsrC"
                     /locus_tag="ACHKUN_001944"
     CDS             complement(20716..21744)
                     /gene="lsrC"
                     /locus_tag="ACHKUN_001944"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO6660200.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            complement(21738..23273)
                     /gene="lsrA"
                     /locus_tag="ACHKUN_001945"
     CDS             complement(21738..23273)
                     /gene="lsrA"
                     /locus_tag="ACHKUN_001945"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO6660201.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLEALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASPKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            23522..24475
                     /gene="lsrR"
                     /locus_tag="ACHKUN_001946"
     CDS             23522..24475
                     /gene="lsrR"
                     /locus_tag="ACHKUN_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO6660202.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            24554..26144
                     /gene="lsrK"
                     /locus_tag="ACHKUN_001947"
                     /pseudo
     CDS             24554..26144
                     /gene="lsrK"
                     /locus_tag="ACHKUN_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
BASE COUNT         6672 a         6215 c         6585 g         6786 t
ORIGIN      
        1 gttttattgc cggattactg catctgaata gcattttccc caaccttgaa aaagcacaag
       61 ctatttttac tccggtaatg aatggttctg taaatatcat gtcaattatg attgctttcc
      121 tggtcgccag gaatatggcg attagctatg agcaagatga tcttttatgc ggattaacgg
      181 caataggagc attttttatt gtttatacgc catatcagat gatagatggg caagcattcc
      241 tgacgaccaa atatctcggc gcgcaggggt tgtttgttgc tgttatcgtt gcattgatca
      301 ccagtgaaat attttgtcgc ttagctcgaa acccaaaaat caccataacg atgccggcag
      361 ctgtaccgcc tgcggtagcg cgttcattta aagttttatt gccaatattt tttgtcatgg
      421 tgttcttttc cgcacttaat tattgcctga cactgatatc cccggcagga ctaaacgatc
      481 ttatttacac attaatccag acgccgctca aacatatggg aacgaatatc tttgcggtaa
      541 ttatcctggg ggctgtgggt aatttcctgt gggtgctggg gatccacgga cctaatacca
      601 catcggcaat tcgagaaacc gttttttctg aggctaatct ggagaatctc tcctgggccg
      661 ctcaacatgg cactacctgg ggcgcgccat atccgattac ctggacttct attaatgatg
      721 cattcgccaa ctgcggcggt tcaggtatga cgttggggtt attgttggct atttttatcg
      781 cttctaagcg tgcggaatac cgtgatctgg caaaaatgtc atttatcccc ggtattttca
      841 atatcaatga accgataatg tttgaccttc ctattgtact taaccccatc atgatggtgc
      901 cgtttattat ggttcccatt gttaactgtg ccattggtta cttctttgtt tcgatggaaa
      961 ttattccacc ggttgcttat gccgtgccct ggactacgcc cggaccttta attgctttcc
     1021 tcggaaccgg ggggaactgg ctggctttac tggttggttt tttatgttta ggtgtggcga
     1081 caatgatcta tttacctttt gttattgccg ccaacaaagt caataacatg acaactaacg
     1141 gataattcca tttacccagg aaggagattg tgatgtttgc agatgaagaa cttgttatgg
     1201 agttactggt caacgcgggg caggcacgtt ctgacgcgat ggaagccata cgttgtgccg
     1261 ggcaaaaaga ctggcagggc gcaactcagc taatggccag ttctgaatct gcctgtcttc
     1321 aggcacataa gattcagacc gcattaatta gtcaggatga aggctgtggc aaaataaaag
     1381 ttaatttaat tctgattcat gcacaggatc atttgatgaa tgcaatatta tgccaggatt
     1441 tagccaggga aattatttca ttaagaaaag aactccatgc ctgatttttt cctccagtgc
     1501 gatagctgga ggaaattatt atactcttaa ggataagtca tgaatataaa gacgcttaac
     1561 gtcagccttt tgtctttttc tattattaca gcattgtttc cattgaacgc gatggcaaca
     1621 aaattaacca tagagcagcg ccttgaattg cttgaaaatg aattgtcgca aaataaacaa
     1681 gagctgaaag caacacagaa tgaactggga gtatataaat cccgactttc gaccttacaa
     1741 aaaagcatca cggaaaacaa atataaatca gcctcgcttg ctgaaatatc agcgacatct
     1801 cccgtcgctg ataacatcaa aaatgaaaac ggtgaacaga actcgtttac cgcagcgcat
     1861 actataaatg gatcgcagca agttgccgtt attgaaagta aaggcgacaa aaccactatc
     1921 gaaagcgtga ccctgaaaga tatcagtaaa tatataaagg atgatattgg gttcagctat
     1981 caggggtact tccgctcagg ctggggtact ggtaatcacg gctcaccaca aacttatgca
     2041 gcgggttctc tgggacgttt tggtaatgag atgagcggtt ggtttgacct gaccttaaat
     2101 cagcgtgttt ataatcagga cggtaaaacg gcaaatgcgg tcgttaccta tgatggcaac
     2161 gtgggtgagc agtataacga tgcctggttt ggtgacagtg ccaatgaaaa tatcatgcag
     2221 ttcagcgata tttacctgac aacgcgaggt tttttaccct tcgcaccaga ggcagacttc
     2281 tgggtcggca aacataaact cccgcaatat gagatccaga tgctggactg gaaaacctta
     2341 accacggatg tcgccgcggg tgtggggatt gaaaactggg cacttggtgt agggctgttt
     2401 gatatgtcct taagccgtga tgatgtcgat gtttactctc gtgatttttc gcgtaccagc
     2461 cagatgaata ccaattctgt ggatgttcgt tatcgcaata tcccgttatg ggatgatgcg
     2521 acattatcat taatggctaa atattccgca cctaataaaa cggatcaaca acaagataac
     2581 gaaaatgaca acagttattt tgaaatgaaa gatagctgga tgctgacttc tgttttacgg
     2641 caaaacctgc aacgcgatac gtttaatgaa tttacgttac aggttgccaa taattcctat
     2701 gccagcagtt ttgccagttt ctcagatgcc agtaatacga tggcgcatgg tcgctattac
     2761 tatggtgatc ataccaatgg gatcgcctgg cgtttaatct ctcagggcga gatgtatctt
     2821 actgacaata ttattatggc taacgcgctt gtctattctc acggcgaaga tgtttatagt
     2881 tatgaaagtg gtgctcatag tgattttgac agtattcgca ctgtaataag accggcctgg
     2941 atctggaata catggaatca gacggggctt gaattaggct ggtttaagca acagaacaaa
     3001 actcagcagg gtgtaacact aaatgaatcg gcttataaaa cgacactctg gcatgcattg
     3061 aaagtgggtg aaagcatttt aggttcacga ccagaaattc gcttctatgg aacgtatatc
     3121 aatattctgg ataacgaatt atcgaatttt aagtttaatg agaacagcaa agacgagttt
     3181 atggccggca tccaggcgga agtctggtgg taatctattt tagttaagga gttgtgtatg
     3241 tcaggattta aaaaaggttt tttatggggc ggtgcggtag ccgcgcatca gttggaaggt
     3301 ggctggaatg aaggaggaaa aggcatcagc atcgctgatg tgatgactgc tggcgctcac
     3361 ggggtgccgc gtgaagtgac agaaggcgtt atcgacgggc ttaattatcc caatcatgaa
     3421 gcaattgatt tttatcatcg ctataaaaca gatattcagt tatttgccga gatgggattc
     3481 aaatgctttc gaacttccat tgcctggaca cgaatctttc cgcaaggtga cgaacaggag
     3541 ccgaatgaag agggtttaca attttatgat gatctgttcg atgaatgcct taagcaggga
     3601 atggaacctg tggtgacgct ttcacatttt gagatgcctt atcatctggt gacaaaatat
     3661 ggtggctggc gaaaccgtaa actgatcgac ttttttatcc gcttcgcatc aacggtcttc
     3721 acgcgctata aagaaaaagt aaagtactgg atgacgttta acgaaatcaa taatcaggtg
     3781 aatttcagcg aaagcctgtg tccatttact aattccggta tcttgtattc gccagaggaa
     3841 gatatcaatg agcgcgaaca aataatgtac caggcggtac attacgagtt agttgccagt
     3901 gccctggcgg tacagactgg aaaatcgatc aatcctgaat ttaacattgg ctgtatgatc
     3961 gccatgtgtc ccatctatcc tctgacgtgt gcacccaacg atatgatgat ggccacgaaa
     4021 gcaatgcatc gtcgttactg gtttactgat gttcatgctc gtggatatta tccgcaacat
     4081 atgctgaatt actttgccag gaaaggattc aacctcgata tcacaccaga agataacgcg
     4141 attcttgcca gtggttgtgt cgactttatc ggctttagct actacatgtc ttttacgaca
     4201 caattttcgc ccgataaccc gcaactggat tatgttgaac cacgagattt ggtcagcaac
     4261 ccttatatcg atacatccga atggggatgg caaattgatc cggcagggct acgttattca
     4321 ctcaactggt tctgggatca tttccagttg ccgctgttta ttgtcgaaaa tggatttggt
     4381 gcggttgacc agagacaagc tgacggcacg gtgaacgatc actatcgcat tgattacttt
     4441 gcttcccata ttcgggaaat gaaaaaagcc gtagttgaag atggtgttga cttaattggc
     4501 tacaccccgt ggggctgcat tgacctggtt tctgccggaa caggggaaat gaaaaagcgc
     4561 tacggaatga tttatgtcga taaagacaac gaagggaagg gaacgctgga acggatacgt
     4621 aaagcgtcgt tttactggta tcgggatctc atcgccaaca atggcgaaaa tatttgagac
     4681 ttgaggaaga tattacggag atgtcaggct ctgtgatcag agcctgacaa taactacata
     4741 gcgtgttgat tataataggg cacgccgagc gcatccgact tatcactgcc agtacccatg
     4801 tgatttaaat caaacggtgg ttgttcctgc gatgggggaa caaccacgac attggcacaa
     4861 gaagtaacaa ctggctgcgt ggtttgttcc gcaacgccct gcgcggaaaa gagaataagc
     4921 gcagctgcta ttgcggatga aagtggtttc atgattgcct cagtgacgtt gtcacgttat
     4981 caactagctg ttgtaatgat ttaatggatg taaaaagcgc gattcgccct gcatattggt
     5041 catccggtat ttatgcggcg gaacatcaaa gtaatttttg aaggttcggg tcagagtttg
     5101 ttgcgactcg aagccatatc gttctgccag atagagtatc ggctcgttac tttccttcag
     5161 cttttgcgcg atttccgtca tcttacggct gcggatgtat tggcctaatg aatgaccggt
     5221 ttctttttta aacatccgtt gcaggtgcca tttagagtaa cctgaacgct ctgacacttt
     5281 ctccagtgac agtggcgatt ccaggttgtc ctcgatccag tccaaaatgc tatgaatggt
     5341 aatagcgtca gtattgcgtc tggacatcgt catacctctt ttttgtttac ggcaggactt
     5401 tcttaagcaa atgctcaagt gttgccactt cgtccgccgt caggtttttt gttaattctt
     5461 gatgcaggtc ctggccaact aattgatggc attgttcgca tattgccgcg ccgctggtgg
     5521 taagttttac cagtacgccg cgcttatcat tcgggttcgg caacctttca acccagcctt
     5581 tacagaccag gcgatccagc atacgggtca gtgctccaag gtcgacagac aacacttttt
     5641 tcagttcaac cggagtaata cacgccgcgc agcggataga gcagagcacc ttaaactgtg
     5701 ccgcggtaat atccagcgga gacagatact cgttaagcag acgatctttc ttctgattaa
     5761 ccatatggat taagcgaccc aatggaataa tttcattgaa cagatcgctg gtacttttca
     5821 cattagttgc cctggcaagt aattagttgc aggggataat attgcccagg caagtataag
     5881 tcaaataaat gaattgaccg atgccacgtt ttgctaaatc ggttcaagtt ttggtcaatg
     5941 cgatgcaagg ttagctaaag agtgacactt tatccttgtt taccccttat acttttcgct
     6001 gataacccag atacacagga taacaaccac caatgttaga tttgtttaaa gcaattggcc
     6061 tggggctggt ggtgttactg ccgttagcta acccattaac aactgtcgcg ttatttcttg
     6121 gcctggcagg caacatgaac agtgccgaac gtaatcgtca gtcgttgatg gcctcggtgt
     6181 acgtatttgc catcatgatg gtggcgtatt acgccgggca actggtgatg gatacatttg
     6241 gcatttcgat tcccggtctg cgaattgcag gcggcttaat cgtcgcgttt atcggttttc
     6301 ggatgctctt tccgcaacag aaagcgattg attcaccgga ggcgaaaagc aagtcggaag
     6361 agctggaaga tgaacccagt gccaatattg cctttgtgcc gctagcaatg ccaagtaccg
     6421 ctggtccggg aaccatagcg atgattatta gctcggcgtc cacagtgcgt cagagttcaa
     6481 ccttcgctga ttgggtactg atggttgcgc cgccgctgat ctttttcctg gtggcggtta
     6541 ttttgtgggg aagcctacgc agctccggcg caattatgcg gctggtgggc aagggtggca
     6601 ttgaagcgat ctcccgtctg atgggattcc tgctggtatg tatgggtgtg cagtttatta
     6661 ttaacggcat cctggaaatc attaaaacgt accattaaaa aaggcccgaa tgggcctttc
     6721 aactattgcg tctgttcttc gagtgtcact ggccagcggc gaaatataat gattgaccaa
     6781 attaacgcgg caaaagcagg caccgcgccc acataaccaa tcatcgacat tgaccagtgc
     6841 aaactcacct gattacctac caacgcaccc gccccgattc caatattaaa tatcccggag
     6901 aatagcgcca tcgcgacgtc ggtagcatct ggtgccagcg ccagcacttt aacctgcata
     6961 ccaagcccga tgatcatcat cgcgatcccc cagaaaatac tcagcacccc gaggtgtatt
     7021 tcactgttcg ccgcaggtaa cagcaatgcc aggcacacca gcaacagcgc aatcgccgta
     7081 ctcaccaacg cagacgcata ctgattaccc agtttaccga aaatcacgct gccaataatg
     7141 cccgcaccac cgagtaataa cagtaatgcc gtggcaaagt tggcgctgaa tcccgcaatg
     7201 ttttgtacaa aaggctcgat atagctgtat gccgtgtaat gggcggtgac aaccaccaca
     7261 gttaacaaat aaatgctcat caatgccggg cggcggaaca atagcgggag gcttttcagt
     7321 gaaccggaat gctcactggg cagtaaggga agtaacttaa tcaggcacaa aagggtgata
     7381 agcgccccaa taccaatcgc gaagaaggtc attcgccaac cgaaatactg gcccacaatg
     7441 cgcccgagag gtaaacctaa gaccatcgcc agtgctgtac cggtggcaat taaactcaat
     7501 gcctgtgctc gcttcccggc cggagccata cggatcgcca gagacgccgt aatcgaccag
     7561 aaaatcgcat gtgcaaaagc cacaccaatg cgactgatca ccagaacggt aaagctccac
     7621 gataaaaacg acagtacgtg gctggcaata aacaccacaa acaggcagat cagtaatttg
     7681 cgccgttcaa cctggctggt cattaacata aaaggcaatg acattagcgc cactacccat
     7741 gcataaatgg tcaacataat gccgacctga gcggtttgca tgtgaaaact ttgcgcaatg
     7801 tcagagagca ggccaacagg gacaaattcg gtggtgttga agatgaaggc ggcgactgcc
     7861 agcgtaacga cccgtagcca cgccactttg cgggaaacag tgtttgttgt cataacagat
     7921 tatcgggtga gttgcgaaaa agtgatgaaa tgatcttaaa acggttaatg atgtgaatac
     7981 aatgcatttg tgatctggat cgcgttttcc gtttctctga cgctgattct gttccgcgtc
     8041 agagagtcgg taacatcacg gtttaatgac gttcagggag gtctggcgtt ttggcggttg
     8101 cgggtatagg tggtctgaca ggggagcgag gcctgttttt caccaggtct tcgtgagtaa
     8161 cttttggcgg cgtttcattt tttacaaaca gtttgagggt cctgcgcgcc atatgtttga
     8221 taattttatc atcacttaat gatgctggca tcaacacgtc attcagatca ctttcattgg
     8281 gattcataaa ttggtgatag tgcaataaca tattggcgaa aaataaaaaa aagtcctgaa
     8341 aatgaggaga ttcttgttct gctaaaaggg ataaaaagca gatgcagact tcgggtagta
     8401 catgtttgtt agaacctaat gaatccatca ggctttgcaa taaatcctct tttaattcgc
     8461 cgtctgttat gtcgagaatg gcggtgatca agttaggatc tttattgacg aaccatctgt
     8521 gaacctcaga gttactccat aatcggggga gtatgacaaa attaccgcga gagataaaat
     8581 tcaggacaag caaatcaaac tggcgtaagt aatctgcagc ggtatggcga tccggaggca
     8641 ggggttcaat atgtggtgta tgcgctcttt tgatgggaac gccaaggttg atcattggaa
     8701 attgttcaat aaaatcattc agaaacagaa atagcacacg ctcgacgccg gtctgaaata
     8761 cgcgccccat agtgacaggc tttgggttgg gagcgcaaaa atattaattt ctccatcatg
     8821 gcgaaaatta tctaaataaa tagggcttaa atttattact acctgaaatt caatattttc
     8881 tttgtgtctt ccattcttga cgggataagt gagtttaaac tgaacctttt cattgtatag
     8941 cgtgtcgtta ccccgaaaag aagtcctctg aatatatgat aagttatcaa ggttattaaa
     9001 tatctttttt gtggctgagt tgatgtttaa agacgtgcgc cactcttcat cacatattat
     9061 tgtacttagc gcatcaataa agtcctttaa aacttctttt acctgaggtg caatattacg
     9121 tacgggaaaa tcatcaggaa gagatatgta attactgatg cttactggcg taaccatatt
     9181 tgccccatat agtcataagt catcggaaca tcactatata gggcatttga gctgcaagaa
     9241 ctatcaaaaa tagctcattg gtaaccctgt tttgccgagt caggcacatc cagtagttga
     9301 ataaatgcct caagcggacg cgttttcgca ccgcgacgcc agaccagcca ggttgttaac
     9361 caacgccatt gctcagctaa cggccacgct tcaacctggt gatgccccgg catactttcc
     9421 agcatagagc gtggaataag cgcaatgcct gctcctgcga ccacacaggc caacatcccg
     9481 tgatacgact ccatctcgtg aatagttccc ggagcggcac cgtcagcatg aaaccagctc
     9541 tcgaagtggc gacgatacga acaattggcg cggaaggcat aaatgttact gccgttaacc
     9601 tgactggcgc ggattactgg cgcatgacct tgtggcgtga cgatcatcag ctcctcgcgg
     9661 tataccggta tcccgtcaat ggcagtatgg ttaatgggcc catcaataaa tgccgcattc
     9721 agttttccct ccagcactcc ttccagcata gtgccggaag ggccggtgga aagtgaaaac
     9781 tgaattttgg gataacgatg gttgaattcc gccagcgtgg ccggaatgcg cactgctgcg
     9841 gtgctttcca gcgaaccaag agaaaataaa ccttgcggct catcgccagc gacaacgctc
     9901 cgcgcttcat ccactaacgc gagaatttgt tggctatagc gtaaaaagtt atgcccggca
     9961 ggagagagac gtaaacgctg attctcgcga ataaacagat ctacccccag ttctgtttcc
    10021 agctggcgta gacgggtagt gagattcgac ggtacgcgat gcacttttgc tgcagcctgg
    10081 gtaatgctgc cagcttcggc aaccgcgttg aacatctcca gttgggtcag atccataaca
    10141 ttctcgattc gtgaataagt ggcttaatat tattcatttt aaagcaagag taaatctgcg
    10201 tatcttcata ccatgactca taaaggagat accccgatga ccattactcc ggcaactcat
    10261 gcaatttcaa taaatcctgc cacgggtgaa caactttctg tgctgccgtg ggctggcgct
    10321 aacgatatcg aaaacgcact tcagctggcg gcagcaggct ttcgcgactg gcgcgagaca
    10381 aatatagatt atcgtgctga aaaactgcgt ggtatcggta aggctctgcg cgcccgtagc
    10441 gaagaaatgg cgcaaatgat cacccgtgaa atgggcaaac caatcaatca ggcgcgcgct
    10501 gaagtggcga aatcggcgaa tttgtgtgac tggtatgcag aacatggtcc ggcaatgctg
    10561 aaggcggaac ctacgctggt ggaaaatcag caggcagtta ttgagtatcg accgttgggg
    10621 acgattctgg cgattatgcc gtggaacttt ccgttatggc aggtgatgcg tggcgcggtt
    10681 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
    10741 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
    10801 ctgaatgccg acaacgacgg tgtcagtcaa atgattaaag attcgcgcat tgctgctgtc
    10861 acggtgaccg gaagtgttcg tgcgggagcg gctattggcg cacaggctgg agcggcactg
    10921 aaaaaatgcg tactggaact gggcggttcg gatccattta ttgtgcttaa cgatgccgat
    10981 ctggaactgg cggttaaagc ggcggtagcc ggacgttatc agaataccgg acaggtttgt
    11041 gcagcggcaa aacgctttat tatcgaagag ggaattgctt ctgcatttac cgaacgtttt
    11101 gtggcagctg cggcagcctt gaaaatgggc gatccccgtg atgaagagaa tgctctcgga
    11161 ccaatggctc gttttgattt acgtgatgag ctgcatcatc aggtggagaa aaccctggcg
    11221 cagggtgcgc gtttgttact gggcggggaa aagatggctg gggcaggtaa ctactatccg
    11281 ccaacggttc tggcgaatgt taccccagaa atgaccgcgt ttcgggaaga aatgtttggc
    11341 cctgttgcgg caatcaccgt tgcgaaagat gcagaacatg cgctggaact ggctaatgat
    11401 agtgagttcg gcctttcagc gaccattttt accaccgacg aaacacaggc cagacagatg
    11461 gcggcacgtc tggaatgcgg tggggtgttt atcaatggtt attgtgccag cgacgcgcga
    11521 gtggcctttg gtggcgtgaa aaagagtggc tttggtcgtg agctttccca tttcggctta
    11581 cacgaattct gtaatatcca gacggtgtgg aaagaccgga tctgacgcga catattcagc
    11641 tctgatatac tcgcaggtct tttcagacct gcggtccagg agtagaaagt ggcagtcgcc
    11701 atggataatg caattttaga aaacatcttg cggcaagtgc ggccgctcat tggtcagggt
    11761 aaagtcgcgg attatattcc ggcgctggct acagtagacg gttcccgatt gggcattgct
    11821 atctgtaccg ttgacggaca gctttttcag gccggagacg cgcaagaacg tttttccatt
    11881 cagtctattt ccaaagtgct gagtctcgtt gtcgccatgc gtcattactc cgaagaggaa
    11941 atctggcaac gcgtcggcaa agatccgtct ggatcaccgt tcaattcttt agtgcaactg
    12001 gaaatggagc agggtattcc gcgtaatccg tttattaatg ctggtgcgct ggtggtttgc
    12061 gatatgttgc aagggcgatt aagcgcacca cggcaacgta tgctggaagt cgtgcgcggc
    12121 ttaagcggtg tgtctgatat ttcatacgat acggtggtag cgcgttccga atttgaacat
    12181 tccgcgcgaa atgcggctat cgcctggctg atgaagtcgt ttggcaattt ccatcatgat
    12241 gtgacaaccg ttctgcaaaa ctactttcat tactgcgctc tgaaaatgag ctgtgtagag
    12301 ctggccagga cgtttgtctt tctggctaat caggggaaag ctattcatat tgatgaacct
    12361 gtggtgacgc caatgcaggc gcggcaaatt aacgcgctga tggcgaccag tggtatgtac
    12421 cagaacgcgg gggagtttgc ctggcgggtg gggctaccgg cgaaatctgg cgttggtggc
    12481 ggtattgtgg cgattgttcc gcatgaaatg gccatcgctg tctggagtcc ggaactggat
    12541 gatgcaggta actcgcttgc gggtattgcc gtccttgaac aattgacgaa acagttaggg
    12601 cgttcggttt attaatgcag tctctcgatc cactcttcgc gcgtttatcc cgttcaaaat
    12661 ttcgctctcg ctttcgtctg ggcatgaaag agcgtcagta ttgcctggag aaaggcgcgc
    12721 cagtcatcga acaacatgcg gcggattttg tcgctaaacg tcttgctccg gcattaccgg
    12781 ctaatgacgg taagcaaacc cccatgcgcg ggcatccggt gtttatcgct cagcacgcca
    12841 ccgcgacctg ctgtcgcggt tgtctggcta agtggcataa cattccgcag ggtgtttcgt
    12901 taagcgaaga acaacaacgt tatatcgtgg cggtaatcta tcactggctg gttgtacaga
    12961 tgaatcagcc atgagaattg cggaaaacga taaaatagca ttcccagggt acctttattt
    13021 gccgccgctg ttagcgaaca tgctatcctt tatgagaatt tacttattac gttgagataa
    13081 ttgccacccg caattgtatg gacagataat taatgcatgt acaccccatt tcaactttcc
    13141 gtttgttcca ggaaggtcat ctgctacgta atagcatcgc tatttttgcg ctaaccacgc
    13201 tgttctattt tattggtgca gagttacggc tggttcacga actttctctt ttctggccgc
    13261 tgaatggcgt aatggcgggg gtgtttgccc gctatgtctg gcttaatcga ctgcattact
    13321 atgcgatcag ttatgtggcg atgctggttt atgatgccat aaccaccgaa tggggactgg
    13381 tttcactggt tatcaatttc tccaatatga tgtttattgt taccgtcgcc ttactggtcg
    13441 tgcgggataa gcgtctgggg aaaaataagt atgagccagt aagtgcctta cggctattta
    13501 attactgtct gattgccgca ttattatgcg ctattgtcgg ggcgattggt tcggtcagta
    13561 ttgatagtct ggatttctgg cctttgcttg cggactggtt cagtgagcaa ttctcaacgg
    13621 gcgtgttgat cgtgccttgt atgctgacgt tggctattcc tggagtgctg ccgcgcttta
    13681 aagcagaaca gatgatgccc gccatcgcgc ttattgtgtc ggttattgcc tcggtagtca
    13741 ttggcggagc ggggagtctg gcgttcccgc tgcctgcatt aatctggtgt gcagtgcgct
    13801 atacgccgca ggtaacatgt ctgttgacct ttgtcaccgg tgcggtggaa atcgtactgg
    13861 tggcaaattc ggtgattgat atctcggtcg gttcgccgtt ctccattccg gaaatgttct
    13921 ccgcacgtct cggtattgcc acgatggcga tatgcccaat tatggtttct tttagcgtgg
    13981 cagcgatcaa ttcgctaatg aagcaagttg cgctgcgagc cgactttgat tttctgactc
    14041 aggtttactc acggtccggt ctttatgagg cgttgaaaag tccatcgctg aaacagacac
    14101 aacatctgac ggtcatgctg cttgatatcg actatttcaa aagcattaac gataactatg
    14161 gacatgaatg tggcgataaa gtgttaagcg tgtttgcccg gcatattcag aagattgtcg
    14221 gtgataaggg gctggtggcg cgcatgggcg gcgaagaatt tgctgttgca gtgccgtcgg
    14281 tgaatcctgt cgatggtctg ctaatggcgg aaaaaatccg taaaggcgtt gaactgcaac
    14341 cattcacctg gcaacaaaaa acgctttatc tcacggtaag tattggcgtc ggtagtggtc
    14401 gcgcatcgta ccgaacgctg accgatgatt ttaataaatt gatggtcgaa gccgatacgt
    14461 gtctgtatcg ctcgaagaaa gatgggcgca accgtaccag cactatgcgt tacggtgagg
    14521 aagttgtctg acatctttat tcattccgcg cgatttgaat gcaatgtgtt ctaccacttt
    14581 atgatccagg aaaaggcgta tttcctcgat taaaccatga tccgcgccac acttttctca
    14641 taaaactgct cattaatcat caggagttga tcaaaacagg ggttcggcgt attaaagttt
    14701 gctaacctgt cgtaccaatt caaacaggtt gtatgactaa tcagaaggga acccattgtg
    14761 aaaacactaa atcgtcgcga ttttcccggt gcacagtatc cagaacgtat cattcagttt
    14821 ggtgaaggta acttcctgcg cgcctttgtt gactggcaaa tcgatctcct gaatgagcac
    14881 accgatctga attctggtgt ggtcgttgtt cgtccgattg aaacttcatt cccaccgtca
    14941 ctgagcacgc aggatggtct gtataccacc attatccgtg gcctgaatga gaagggggaa
    15001 gcggtcagcg acgcgcgtct gattcgctcg gttaatcgtg aaatcagcgt ctacagtgaa
    15061 tacgatgaat tcctgaaact ggctcacaac ccggaaatgc gctttgtttt ctctaatacc
    15121 accgaagcgg gtatcagcta ccatgcgggc gataaattcg atgacgcgcc agcggtaagt
    15181 tatccggcaa aactgacccg tctgctgttc gaacgtttta gccatttcaa cggtgcgctg
    15241 gataaaggtt ggatcatcat cccgtgtgag ttgatcgact acaatggcga cgcgttgcgt
    15301 gaactggtgc tgcgctatgc gcaagagtgg gcactgccag aagcatttat tcaatggctg
    15361 gatcaggcta acagcttctg ttctacgctg gtggaccgta tcgttaccgg ttatccgcgc
    15421 gatgaagtgg ctaaactgga agaagagctg ggttatcacg atggttttct cgacaccgct
    15481 gaacactttt acctgtttgt gattcaggga ccgaaatcct tagcgactga actgcgtctg
    15541 gacaaatatc cgctcaacgt gctgattgtt gacgatatta aaccgtacaa agaacgcaaa
    15601 gtggcaatcc tcaacggtgc gcacaccgcg ctggtgccgg tggcgttcca ggcagggctg
    15661 gataccgtag gtgaagcgat gaacgatgct gaaatttgcg cattcgttga aaaagctatc
    15721 tacgaagaaa ttattccggt actggatttg cctcgtgatg aactggaatc tttcgccagt
    15781 gcagtcaccg ggcgtttccg taacccgtac attaagcatc agctgctgtc tatcgcgctc
    15841 aacggtatga ccaagttccg cactcgcatc ctgccgcagc tgctggcacg gcagaaggca
    15901 aaaggcacac ttccggcgcg ccttactttc gcattagcgg cattgattgc cttctatcgt
    15961 ggtgagcgta acggagaaac atatccggta caggacgatg cacactggct ggaacgttat
    16021 cagcaactct ggagccagca tcgtgaccgt gttatcggta cccaggaact ggtagcgatt
    16081 gtgttggcag aaaaagacca ctgggagcag gacctgacgc aagtgcctgg tctggtcgag
    16141 caggttgcta atgacctgga tgcgattctt gaaaaaggga tgcgcgaagc tgtacgtccg
    16201 ttgtgctaat aacaccgtta cttctgaacc cggtttcgac cgggtttctt atttcactat
    16261 attagttaca acatcctatg tgcctttatc attaacagat gccacatgca gcgcctgtta
    16321 atgttgtaag cttgtaggta atttgtaagt agcgtcttga gaatgtgatc gtaatcacgt
    16381 ttaatagggc gactttttca cgcctgaaga taaccatgat tgttcgtcca caacaacact
    16441 ggctgcgccg tatttttgtc tggcacggct cagtattatc caagatatca tcgcgcttac
    16501 tactcaattt tctcttttct atcgctgtta ttttcatgct gccctggtac acgcatctgg
    16561 gcatcaaatt caccctcgca ccgttcagca ttctcggtgt cgccatcgcc atttttcttg
    16621 gttttcgtaa taatgccggg tacgcccgtt acgttgaagc gcgaaaactt tgggggcagt
    16681 tgatgattgc ctcacggtcg ttactgcgcg aggtaaaaac gacattgccg gattcggcaa
    16741 gtgtaaggga gtttgcccgg ttgcaaatcg ccttcgccca ctgtttacgc atgacattac
    16801 gcaaacagcc acaggcggaa gtgctggctc attatctcaa gactgaagat cttcagcctg
    16861 tactggcttc gaactctcca gctaaccgta tcttgttaat aatgggggag tggttggcgg
    16921 ttcagcgccg caatgggcag ctttcagata tccagtttat tagcctcaac gatcggctta
    16981 atgatatttc agcggttctg gcaggctgtg agcgcattgc ctacacgcca attccctttg
    17041 cctacaccct gattttgcat cgtaccgttt atctgttttg tatcatgctg ccgttcgcgc
    17101 tggtcgtgga cctgcattac atgacgcctt ttatctctgt gctgatttcc tacactttta
    17161 tttcgctgga ttgtctggcg gaagaactgg aagatccgtt cggtactgaa aacaatgatt
    17221 taccgctgga tgccatctgc aacgctattg aaattgacct gttacagatg aacgatgaag
    17281 cagaaattcc agcaaaaatt cttcccgatc gtcattacca gctgacgtga taattactcc
    17341 gtacggcggg caacaataaa cagacgcgga aatgccagca gtatctgtcc gttctcttgc
    17401 agtggatact gctcttccag catctgatgg tagcgcgtaa gaaaaagctg ctgttcgctc
    17461 tcggtcagat cctgtaacca cggacgtaat ccagtggcgg tcacccaatc gataatcgcc
    17521 tggtgcgacg gcatctggtg atagtaagtg gttcgccaga tatcgacctc acatccggct
    17581 tcgctcaaaa tatcgtagta agcatgaacg ccagccaacg gctcacgccc gcgatccggg
    17641 tagttttgtt cccaggcaac ttcgcgcatg agcacatggg tcggctccag ccagttatct
    17701 ggcatctgta ctgccagcac gccctgcgga ttaagtaaag aaaccagatg aggaaacaat
    17761 tcgtagtggt cgggcagcca ttgcagtgag gcattagcaa aaatcagatc gagtgcctgt
    17821 tccggttgcc gtttcgttat ggtgaaccac cgcctgtacg gctttcatca tcgccaccgg
    17881 atggtcagac tggaaaatat tacgccccat atccacacca gaagcgccct gatcgatagc
    17941 ctgccagcac atttccagcg cctcgcgctc cggtaatttt ttaccgccag caataacaat
    18001 gggtaccgga catccggcaa caatccgttc aaaacctttt tcgacataat aggttttgat
    18061 aatttgcgcc cccatttcag cggcgattcg agtcgcgagc gagaaataac gctgatcgcg
    18121 caccatatct ttgcccacgc cagtcacggc catggtcggc attcccactt tcattccggc
    18181 atcaaccagc tgaataatat ttttgatcga ctgatgttca tattcgctgc cgatataaac
    18241 ctgcgccgcc accgcgcaac tgttcaggcg cacggcatca tccatcgata acgccacggc
    18301 ttcattactt aattccgcca gaatagagtt cgctcctgac gcccgcagta ccaccggctt
    18361 attggtcgct gggggaacta cgctgcgcaa aatgccgcgc gtacacatta atacatcggc
    18421 atgttcaaac agcggggcga tatttatatc aatgcgttca agtccggtag tcggcccctg
    18481 aaaataacca tggtcaaagg ccagcatcac ggttttaccc gttttcggat taaatatccg
    18541 cgataagcgt gactgcattc cccaatccag cgcaccgcaa cctttcagga taaaagggat
    18601 attttgttgc gattgatcgg tacgaaaatc tttaccatct ttaatatcgt ctaaatctgc
    18661 cataacttac tccggttaag taatgcacat cagaaatcgt atttgccgat attctctttg
    18721 ttgaatatca cgcgctccgg taacagtacg atgccattac catccgcttc gtagtcatag
    18781 ccctgaacgc tgtttggcga gacttccacc tgacctacgc cctggatatc cagcttgtcg
    18841 cccgttttca ttgatccttt tttcaataat gcatccgcga catacactga aattttgcct
    18901 tgctgaacca catcccacag gccaaattct ttcaccgtgc cgcgttccac atatggacgc
    18961 atcacgtttg gcgtactgaa tccgacaatc gcaactttgt catttttcag gttttctgcg
    19021 gcttgtgcgg cagcgggcag ggcgttggcg tcgggggcga taatggcgtc gagatcgcta
    19081 tacgctttta atattccttc tgcggtttgt aatgatttag tggcatcgtt atagccaaac
    19141 tgcgtagtga caatttccca gccaggatgc tctttggcga ttttcgcttt cgcttctttc
    19201 acccactggt tttggtccgt aacggtgggg cttgagtaga aaaacgcgac tttggctttg
    19261 tctttattca cctgacgcgc cgccatatcc accaacatac ctcccaactg ggcgggcgtt
    19321 ccctgattaa tgtagtaaga gcggcactcc ggtttagtat cagagtccca ggtcagcact
    19381 ctcacaccac gttgcatggc gcgtttcagt gccggacaca agccatcagg cgaaaccgca
    19441 gaaacgataa tggcgttata accttgattg acgaagttat taatcaactg tacctgacca
    19501 gaaacactgg gttctgtcgg cccgtcgtag gtcacatcaa cgcccagctc tttacccgct
    19561 tgttgtgcgc cgttgccacc gctggtaaaa aatcccacgc caaccagttt gggaataaat
    19621 gcaatacgct ctgcggcctg cacattcata gagattgcgg caatgccaag agcgctaagt
    19681 aaggcgattt tcttaaagcg atgaagtgtc atgaagatat cctttatggc aatgggttat
    19741 tggcccgacg cgccagccac tctttaattt gctggcgatg caggctaacg gaacgaccta
    19801 cgacaacgac gataagtagc gcaccggaaa gggcgctgga cacctgattt ggcactcctg
    19861 ccatttgcaa accttgctgc aaatatccca ctaataaaac cgcaatggcg gtgccgataa
    19921 tggaaccgga accaccataa atattggccc cgccaagcac cacggcggtg atggcgggca
    19981 tcagaaacga cgcaccgaga tcggaacgtg ctgaaccaaa atacgatacc agcagcacag
    20041 cggcgaccgc agacgccagc cccgtcatgg catagagcgc acataaggtg cggttaactg
    20101 gaatcgcgct ataaagcgcc acgcgcgggc tttgcccaat caaaaacaca ttacgtccgg
    20161 catgggtttt atgcagccag agccagaaaa cgaggagaca tatcaggaag ataatcagcg
    20221 gaacggggag tcccagcaca tccaggttag cgaaatctgt aaacgccatc gggaatccac
    20281 caataccttc gtaccccgtc gctccggcca taccggaaag cagcagagcg cttccggcaa
    20341 acagatacag cgtgccaagc gtaatcacca gcgggttaac tttggtatag ataattaatc
    20401 cggcgttgat cagcccgcac aatgcgccga gcagtaaggt caggagtatc gccagcggca
    20461 tcggcacacc actttgaaac agtacgccca atgcaatggc gcagaggccg atggtcgaac
    20521 caaacgaaat atcgatcccg ccactgacaa tcaccatcgt cagcggtagg gcgacaatgc
    20581 caatgcagat aaagtcactg gtgctgaaca gcaacatatt gagatctaac attcgcgggt
    20641 taattgcacc aaatgcgaca atctcaataa cgagcagtgc ggcaagagcc agttcccaac
    20701 cgtagcgaat acgcattatg cggcctcccg ttttttacct gacgaagcgg gtttaacgga
    20761 tggcggtggc gtcataaagc gggcatattt ttgccgccgt agattacgtt ccagcgcaca
    20821 acgcaggcgt ccatcaaaca ccagcaccgc cagcagcacg aggcccgcga taaaatcgtt
    20881 ccaccatgcc ggaatgcgca acagcaccag tacgctatcg atctgcgtca ggaaccatgc
    20941 gccgagtacc gcaccgatga tcgcaccgga accgccgagc aaactaatgc cgcccagcac
    21001 gcaggctgca attgctttca tctccagccc ggtaccggtc tggttgggga taaaaccaat
    21061 ctgcgaagca aacacaattc ccgccagtgc cgccatgcag ccgttcaacg aaaatgccac
    21121 aatgcgaatg gcttccgtac gaacacccag ttgacgagcg ccctgtaaat tatcgcccgt
    21181 ggcataaaaa ctgcgtccaa acgccgtctt tgccagcagc caggccataa atgccaccag
    21241 aattatcgtc aaccaaccaa tcgcggaaac gccaaacagc agcggtgcgg agagctgttt
    21301 cagttcggcg ggtaaccctt caatccattt accgccagtc cacagcaaca tgatgcctct
    21361 gtacaaccct aacgtgccaa gggtggcaac aatggcaggg atctttagcc acgcgaccag
    21421 gacaccgttg aaaaatcccg cgagcaaacc aagcagtaaa gtcgcgacac aggcaacagg
    21481 tagtgaatat cctgcgttca gtaacatccc caacagcacc gcgcacattc cggtaatcga
    21541 acccactgaa acatcaatat tgcgcgtaag cattaccagt gtcgcgccca ttgccagcag
    21601 gatcaggatt tgcgcgctgc tataaaccat ggtcagcgtt tgcacactta aatactgacg
    21661 gtcgagaaaa ccgggtaata caaacagcag caccaccgcc agcaatgccg tgatttcacg
    21721 gttgttctga ataaacttca gcatgatgcc tcctgacgct gactatcgcc gaaggcaacg
    21781 cgcataatag tctcgacatt aatatcgcgc ccggtcagtg cagagtgggt aatttcgccc
    21841 tgatgcatca catacacacg atctgtcatc agttcgatct cttccagatc agaggagata
    21901 agcagcacag ccacattctg tgcggagatg ctgcgcaaca gctggtagat atcattacgg
    21961 gccgagacat ccacgccgcg cgtcggctca tcgacaatca atgcttgcgg cgaagcttcc
    22021 aggcatttgg caatgaggat tttttgctgg ttgccaccgg ataatgtccg tgcagcttgt
    22081 tccggttggt taaatttaat attcagcgcc cgacgataac gttccagggt ggcattatct
    22141 ttcgcggttt tcgcccagaa tccacgaagg ttatgagtaa gggcgcagac gttccaggcc
    22201 agcgaagcat cgagattcag tccggatgac tggcgatctt ccggcagata aaccagaccg
    22261 cgcagtaaac gttctccagt ggataattta ttgatctctt taccattcag cataatgcgt
    22321 ccgctacgca aagtacgcag accatagaac gtctcggcca gttctgtgcg tccggccccc
    22381 accagcccag ccaggcccag aatttctccg gcattgagcg tcatgctgac attcctgaaa
    22441 ccttcgccgg tcagattttc cagtgtcagc accggcgttc cggcagcatg ttgtgggcgg
    22501 ttaccaggta actccagcca taattttggg ctggcagaga gcgatttttc ccgtaccact
    22561 ggcgtgatgg cctgaataat gtcgtcggta gacagttcgc tggttttgcc gcttaaggcg
    22621 atggttccgt cgcgcatcac gctaattcga tcggcaatct ggcgaatttc cggcagctta
    22681 tgcgagataa aaacaatacc cacgccagta gcaagcagct cttgcaagcg agtaaacaag
    22741 cgttcggttt ccgcaggggt aagcgaggcg gtaggttcat cgaggatcag aatccgcgag
    22801 tcgcgcatta gcccgcggag gatttccacc atttggcgat cggcgacatc cagcgatcct
    22861 gccagactat gcagatcaaa ctggcagccc agcgcctcca gcaagttctt cattttttgc
    22921 atggagagct gtttttttgc catcccaaac aggatgtttt cttttatcga caggcttggg
    22981 aaaagcagcg gttcctgggg aacgagataa atgcccagct gatgagcatg aactggcgtt
    23041 aatctggcgt agttgttgcc cccaatctcc agcgtaccgc tatcagcagg ggtaataccg
    23101 gcaataatct tcattaacgt cgatttaccg gcaccattgc cgccgagcag ggcgtggacc
    23161 tccccctgat gcaacgtaaa atcgatgcct ttcaggacat tgacccctga atactgttta
    23221 taaaccgagc gggcgcaaag tagcggtaac gcgcgggtat cactcgtttg catatttccc
    23281 ccgttcagtt ttgcaggtga gttttgaaca aatgtatttc tgcttttaat ttgttcataa
    23341 ccttaggtgg acattgcaca tatttccgac gaatagatca caatttatgc tattttgatt
    23401 ttcacggttg cgtttgttca tgctcgtaga gtcaaactgt ggctgccatc acagatataa
    23461 atgcgcaaga actgaacaat tgcattaaag atttaaatat gttcaaagtg aagaatgaat
    23521 tatgacaatc aacgattcgg caatttcaga acagggaatg tgtgaagaag aacaggtcgc
    23581 gcggatcgcg tggttttact atcacgacgg gctgacccag agcgagatca gcgatcgtct
    23641 cggcctgacc cgtttgaaag tgtcgcgctt gctggagaaa gggcatcagt ccggcattat
    23701 tcgcgtacag attaattctc gctttgaagg ctgtctggaa tatgaaactc aattacgtcg
    23761 tcagttttcg ctgcaacatg tccgggtgat ccctgggctt gcggatgctg atgtcggcgg
    23821 gcgactaggg ataggcgcgg cgcatatgtt gatgagttta cttcaaccac aacagatgct
    23881 ggcgattggt tttggcgagg caaccatgaa tacgctgcaa cgcttaagtg gttttatttc
    23941 gtcacagcaa attcgcctgg tcacgctctc cggtggcgtc ggttcttata tgacgggtat
    24001 cgggcagctt aacgcggcgt gcagtgtgaa tattattccg gctccgttgc gggcatcctc
    24061 cgctgacatt gcccgtacgc taaaaaatga aaattgcgtc aaagatgttc tgttagccgc
    24121 gcaagcagcg gatgtggcga ttgtcggcat tggggccgtc agtcaacagg acgacgcgac
    24181 gatcattcgc tccggttata tcagccaggg cgaacagtta atgattggtc gaaaaggggc
    24241 ggttggcgac attttaggct acttttttga tgcaaaaggt gacgttgtca cggatattaa
    24301 aatacataac gaactgattg gcttaccttt aagcgcgctg aagaccatac ccgtccgggt
    24361 tggcgtggca gggggagaaa ataaagccga agcaattgcc gctgcaatga aaggcggtta
    24421 tatcaacgca ctggttaccg atcaggatac agcagcggcg attttacgta gttaaatttg
    24481 catgacctta ttaccgggaa tgatggagaa cgtatagagg aacagacaga acgcataagc
    24541 cgaggataat ctaatggctc gactctttac cccttcagaa tcaaagtatt acctgttggc
    24601 gctggatgca ggcaccggaa gtattcgggc tgtgattttc gacctgaaag gcaatcaaat
    24661 agcagtggga caggcggagt ggcggcatct ggcagtaccg gacgttcctg gttctatgga
    24721 atttgatctc aacaaaaact ggcaactggc gtgtgagtgt atgcgccagg cgctgcacaa
    24781 cgccggcata gacccggagt atatcgctgc cgtttcggca tgttctatgc gtgaaggcat
    24841 tgttttatat aacaatgaag gaacaccgat ctgggcctgc gccaatgtgg atgccagagc
    24901 agcgcgcgaa gttagcgaac ttaaagaact gcacaacaat acctttgaaa acgaagttta
    24961 tcgcgcgacc ggacaaacac tggctttaag tgccatcccc agattacttt ggctggcgca
    25021 ccatcgttcc gatatttacc gtcaggcatc aaccatcacc atgatcagcg actggctggc
    25081 ctatatgctc agcggcgaac tggcggtgga tccctctaac gctggcacca cgggactttg
    25141 atctaaccac ccgtgactgg aaacctgcat tgctggatat ggctggcctg cgtgccgata
    25201 ttctttctcc tgtcaaagaa accggcacat tgctgggcgt ggtaagttca caagcggcgg
    25261 aactctgcgg tctgaaggcg ggcactccgg tggtcgttgg aggaggcgac gtgcagcttg
    25321 gttgccttgg gttaggcgtt gtgcgtccgg cacaaaccgc ggttcttggc ggcacattct
    25381 ggcagcaagt tgtaaattta gccgcgccgg tgacagaccc agaaatgaac gtgcgcgtta
    25441 atcctcatgt tattcctggc atggtacaag ctgaatctat aagctttttt accggactca
    25501 ccatgcgctg gttccgcgat gctttctgtg ccgaagaaaa actgattgcc gaacgtttag
    25561 gcatcgacac ctatacgctg ctggaagaga tggccagtcg ggtgccgcct gggtcgtggg
    25621 gcgtaatgcc gatcttctcc gacagaatgc gctttaaaac ctggtatcac gctgcgcctt
    25681 cctttattaa cttgtccatt gacccggata aatgtaacaa agcgacattg ttccgtgcgc
    25741 tggaagaaaa tgcggcgatt gtatcagcgt gtaacttgca gcaaattgct gatttctcga
    25801 atattcatcc cacctcgctg gtttttgctg gcggaggttc aaaagggaaa ttatggagtc
    25861 aaattctcgc tgatgtctcg ggattacccg tcaatattcc ggtagtcaaa gaagccaccg
    25921 cattaggatg tgccattgca gctggcgtcg gtgccggaat tttttcatca atggcagaaa
    25981 ccggagaacg cctggttcgc tgggaacgga cgcacacacc agacccggaa aagcatgaac
    26041 tttatcagga ttcacgcgat aagtggcagg cagtttatca ggatcagctg gggctggttg
    26101 atcatggact gacgacgtcg ttatggaaag cgcctgggtt atagtttcat tgacggccac
    26161 atcatgatga ccgtggctgt tagtttttga gtatagtttt tcttatttcc cttcttttag
    26221 agctggcttg ttaaatgcca gcttgcagat tcatcgtt
//
LOCUS       DAZFRJ010000052        26064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000052 DAZFRJ010000000
VERSION     DAZFRJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.052"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHKUN_001948"
     CDS             complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHKUN_001948"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO6660203.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHKUN_001949"
     CDS             complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHKUN_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO6660204.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHKUN_001950"
     CDS             complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHKUN_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO6660205.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHKUN_001951"
     CDS             complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHKUN_001951"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO6660206.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHKUN_001952"
     CDS             complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHKUN_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6660207.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHKUN_001953"
     CDS             complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHKUN_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO6660208.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHKUN_001954"
     CDS             complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHKUN_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO6660209.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHKUN_001955"
     CDS             complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHKUN_001955"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6660210.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHKUN_001956"
     CDS             complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHKUN_001956"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO6660211.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHKUN_001957"
     CDS             complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHKUN_001957"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO6660212.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHKUN_001958"
     CDS             complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHKUN_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO6660213.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHKUN_001959"
     CDS             complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHKUN_001959"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO6660214.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHKUN_001960"
     CDS             complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHKUN_001960"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO6660215.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHKUN_001961"
     CDS             complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHKUN_001961"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO6660216.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHKUN_001962"
     CDS             complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHKUN_001962"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO6660217.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHKUN_001963"
     CDS             complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHKUN_001963"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO6660218.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHKUN_001964"
     CDS             complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHKUN_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO6660219.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHKUN_001965"
     CDS             complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHKUN_001965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO6660220.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHKUN_001966"
     CDS             complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHKUN_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO6660221.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHKUN_001967"
     CDS             complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHKUN_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO6660222.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23548..23634)
                     /locus_tag="ACHKUN_001968"
     CDS             complement(23548..23634)
                     /locus_tag="ACHKUN_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO6660223.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHKUN_001969"
     CDS             complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHKUN_001969"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6660224.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHKUN_001970"
     CDS             complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHKUN_001970"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO6660225.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25983..26027
                     /locus_tag="ACHKUN_001971"
     CDS             25983..26027
                     /locus_tag="ACHKUN_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660226.1"
                     /translation="MKRNDKILHYEGLN"
BASE COUNT         6143 a         7496 c         6312 g         6113 t
ORIGIN      
        1 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac
       61 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
      121 aagaccgact agcgacatcc actcaccggg ttgcccttct ttaccccacg gctccccttc
      181 ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
      241 tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa gttcacgcac
      301 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
      361 cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct cgttgcgaat
      421 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
      481 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
      541 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc accggtttgc
      601 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc
      661 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
      721 cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc cagcatcgct
      781 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
      841 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
      901 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
      961 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
     1021 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
     1081 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
     1141 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
     1201 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
     1261 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
     1321 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
     1381 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
     1441 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
     1501 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
     1561 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
     1621 tctacggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc gagcacaata
     1681 tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata acctgcctga
     1741 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg cttaataccg
     1801 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
     1861 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
     1921 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
     1981 acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt atcagcagta
     2041 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
     2101 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
     2161 ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
     2221 tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc
     2281 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
     2341 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
     2401 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
     2461 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
     2521 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
     2581 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
     2641 gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
     2701 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accgacagtc
     2761 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
     2821 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
     2881 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
     2941 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
     3001 cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgt
     3061 gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc catctccggt
     3121 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
     3181 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
     3241 gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
     3301 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
     3361 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
     3421 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
     3481 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg
     3541 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg cttccagcaa
     3601 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
     3661 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat
     3721 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
     3781 tccactcacg ttattgccat cccgatcgta ttcaaacgta tttagcgcac aaatcttatc
     3841 attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag tgcttaagaa
     3901 aacagcgttc gcaccgtttt ttatagctct tataaaattt aaaaaaaaac gactggttca
     3961 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
     4021 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
     4081 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
     4141 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
     4201 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
     4261 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
     4321 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
     4381 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
     4441 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
     4501 tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg
     4561 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
     4621 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
     4681 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
     4741 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
     4801 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
     4861 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
     4921 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
     4981 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
     5041 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
     5101 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
     5161 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
     5221 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
     5281 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
     5341 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
     5401 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
     5461 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
     5521 tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
     5581 cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat cgttcgctgc
     5641 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
     5701 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
     5761 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
     5821 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
     5881 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
     5941 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
     6001 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
     6061 atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt caacagcatt
     6121 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
     6181 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
     6241 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
     6301 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
     6361 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
     6421 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
     6481 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
     6541 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
     6601 cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
     6661 cggacgacca cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
     6721 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
     6781 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
     6841 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
     6901 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
     6961 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
     7021 gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc ctaccggatt
     7081 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
     7141 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
     7201 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
     7261 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
     7321 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
     7381 acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
     7441 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
     7501 cgccttgatc attgttgttc tgcctgtgca tgattttgtt gctgagtaga ttcctctggc
     7561 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
     7621 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
     7681 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
     7741 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
     7801 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
     7861 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
     7921 gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
     7981 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
     8041 tctatttgcg tctggatgat gttgacatct tgggtcataa aggtacccgg ctcacccaat
     8101 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
     8161 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
     8221 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
     8281 ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata
     8341 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
     8401 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
     8461 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
     8521 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
     8581 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
     8641 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
     8701 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
     8761 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
     8821 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
     8881 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
     8941 ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
     9001 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
     9061 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
     9121 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
     9181 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
     9241 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
     9301 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
     9361 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
     9421 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
     9481 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
     9541 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
     9601 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
     9661 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
     9721 ccagcgggaa ttcaccgagg aaatcaaaac ggcgcccagt cccctggaag ctctccagcg
     9781 cacgtaaaat agcctcgtca tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
     9841 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
     9901 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
     9961 cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc
    10021 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa
    10081 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    10141 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    10201 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    10261 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    10321 ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    10381 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    10441 cgacaatttc cgggttatag gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    10501 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg
    10561 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    10621 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    10681 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    10741 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    10801 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    10861 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    10921 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    10981 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    11041 ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    11101 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccgcttcggc
    11161 atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga tagtgactga
    11221 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    11281 accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    11341 cggcagcgcc agtacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    11401 gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat tggttaagcc
    11461 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    11521 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    11581 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    11641 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    11701 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    11761 cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    11821 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    11881 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    11941 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    12001 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    12061 agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga catcgcgcga
    12121 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc cagttcttcg
    12181 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    12241 gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    12301 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    12361 atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat accgataatg
    12421 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    12481 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    12541 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    12601 agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    12661 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagcacg
    12721 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    12781 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    12841 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    12901 gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg
    12961 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ttttagcgcc
    13021 gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    13081 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    13141 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    13201 gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt tctgccactt
    13261 ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt
    13321 cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg ccgcccagca
    13381 acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta ctgccgacgt
    13441 tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc acttcaaagc
    13501 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag
    13561 ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc ccggaaagtt
    13621 tcatctcttt cacgttcagc actttctcgc ctttcactcg cagccaggtt tctccctgct
    13681 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatcagcac
    13741 cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc gcattttcgt
    13801 aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa cggatagcgg tccatatgat
    13861 cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct
    13921 ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc atcaacgctg
    13981 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    14041 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    14101 cttcgcggca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    14161 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    14221 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    14281 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    14341 ccacgcagga aagcccagtg aggcccaggc cgataatgac gacattttta ccctgataat
    14401 cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca tcagcgaaat
    14461 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    14521 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    14581 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    14641 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    14701 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    14761 cgtgcagaca atgaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    14821 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    14881 cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    14941 aatgacgaag taagccagca gaatgtagaa cagccccagc tgcggcatca catctttaaa
    15001 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc
    15061 gatacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca accctttggt
    15121 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    15181 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gtacggagat
    15241 cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    15301 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    15361 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    15421 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    15481 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta
    15541 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    15601 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    15661 tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    15721 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    15781 acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga accgacattg
    15841 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    15901 cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    15961 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    16021 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc
    16081 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    16141 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    16201 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    16261 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc agcttccggg
    16321 cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag ttcaataact
    16381 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    16441 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    16501 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    16561 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    16621 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    16681 tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg caacaaacag gcagcccggc
    16741 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    16801 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    16861 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    16921 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    16981 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    17041 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    17101 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    17161 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    17221 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    17281 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttta
    17341 acagatcagc cagcgggtag ccaagtgcca gcagtgtcgc cagcgcgagc agcaggttgc
    17401 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa cttgaactaa
    17461 agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt
    17521 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    17581 gccagcggcg gcccacttca tcgtcggcgt taacaatcgc ctgaccacaa tgatgttcag
    17641 aataaagcag ccatttcgcg gcttcgtagt gttccatatc accatgataa tcaaggtgat
    17701 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgctgta
    17761 ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgatcc accagccccg
    17821 ccagctcatg ctgaacatcg actgccgaac cggtggtatt ttctgtcggg atcactttcc
    17881 ccagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    17941 actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    18001 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    18061 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    18121 cacctttcgc ctctgcaata atggcagtca caccttgcgc tatcgcctgc gggatatatc
    18181 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    18241 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    18301 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    18361 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    18421 atgatggcac caaagaccgg cgctgaaacg gcgccgccgt agtatttacc cgcctgcgga
    18481 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    18541 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    18601 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    18661 acgctttcca tcatatgcac cacagtgcgg acaatggatt ccgggaagac acgttcaccg
    18721 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    18781 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    18841 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    18901 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    18961 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    19021 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    19081 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt
    19141 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    19201 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    19261 gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    19321 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    19381 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    19441 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    19501 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    19561 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    19621 agttttttga tgtagtccgc catgtcaggg ttcacctgac gcgccagata aataaagcgc
    19681 cctttcgggt tggcgttaat gcgggctgaa agctgatcca gcggaatatt gagcgcgtta
    19741 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    19801 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    19861 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    19921 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    19981 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    20041 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    20101 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    20161 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    20221 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    20281 cgggtatggt gcgccgtggt taccacagtc accgccgtca aaataatgca aatgaacagg
    20341 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    20401 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    20461 atgcattcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    20521 cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt
    20581 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    20641 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    20701 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    20761 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    20821 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    20881 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    20941 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    21001 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    21061 gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    21121 tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    21181 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    21241 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    21301 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    21361 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    21421 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    21481 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    21541 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    21601 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    21661 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg tagggggtaa
    21721 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    21781 agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    21841 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    21901 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    21961 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    22021 cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    22081 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    22141 ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    22201 agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca tctctgcatg
    22261 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    22321 tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga atagtcttat
    22381 ttactttaag gatattgatg tccattcccg tactctacgc gccagaggga aattcacctg
    22441 gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca ccgcggcgat agagattacg
    22501 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat
    22561 ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgccagca ccgggcactg
    22621 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    22681 accatcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    22741 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc
    22801 ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    22861 acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    22921 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    22981 ggcaccaacc acgctggtga aatgttcacg atcgagggcg cggtccagcg cgacaatcgg
    23041 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac
    23101 aataatggca tcaacctgac gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg
    23161 ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgctcaag
    23221 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    23281 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    23341 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    23401 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    23461 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    23521 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    23581 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
    23641 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    23701 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    23761 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
    23821 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    23881 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
    23941 ttgtgtaatt gcttttcgat ctgctcaagc actttttcat cgcccacggt ctggatggtc
    24001 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    24061 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    24121 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    24181 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    24241 acaccaggcg attattgcac acctgttcca gcgcctcgct aagtttgctt tccagctcat
    24301 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
    24361 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
    24421 tcaccatccc cagatagcgg ttattgagat tcaccaccag taccggcaac tcgtattgca
    24481 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
    24541 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    24601 tgccgaggcc accggaattg atccagcgac gcggtttgtc gaatggataa taaagtgcag
    24661 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc
    24721 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
    24781 actgacgagc gcgccactgt tcaatttgct gccaccagtc gcggatctca tccagtggtt
    24841 gatgggcgga ttcttgcgac aagagttcaa gcatttgttc gaggacctgg cgagcatccc
    24901 ccacaatcgg gatatccgca gtcacggttt tagaaatgga agtaggatca atatcgatat
    24961 gcagaacagt ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc
    25021 gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
    25081 cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc cggaaacgcc cccagcccca
    25141 tcaatgagga aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
    25201 agcccgccat gattgcccca ccgcctacgt agacaaccgg tttttttgcc gctaccagcg
    25261 tttgcagagc gcgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca
    25321 tactgaccga ctcaggccag acatagggta atttgttcgc cggattaaga atatctttcg
    25381 gtaaatcaac gaccactggt cctgggcgac cacttgccgc cagccagaaa gcctttttca
    25441 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
    25501 aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
    25561 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
    25621 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg
    25681 ccagaccatc ggccatatgc actgccgcct gctcatgacg aactaaaaca tgatcgatac
    25741 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
    25801 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    25861 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    25921 tgcttaagca attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa
    25981 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgataa taaatcggct
    26041 gaatcccaca acttacagca attt
//
LOCUS       DAZFRJ010000053        25708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000053 DAZFRJ010000000
VERSION     DAZFRJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.053"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..424
                     /gene="bfr"
                     /locus_tag="ACHKUN_001972"
     CDS             <1..424
                     /gene="bfr"
                     /locus_tag="ACHKUN_001972"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO6660227.1"
                     /translation="LVAINQYFLHARMFKNWGLMRLNDVEYHESIDEMKHADKYIERI
                     LFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLREAIAYADSVHDYVSRDMM
                     IEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(421..888)
                     /locus_tag="ACHKUN_001973"
     CDS             complement(421..888)
                     /locus_tag="ACHKUN_001973"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO6660228.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1267..1578
                     /gene="rpsJ"
                     /locus_tag="ACHKUN_001974"
     CDS             1267..1578
                     /gene="rpsJ"
                     /locus_tag="ACHKUN_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO6660229.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1611..2240
                     /gene="rplC"
                     /locus_tag="ACHKUN_001975"
     CDS             1611..2240
                     /gene="rplC"
                     /locus_tag="ACHKUN_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO6660230.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2251..2856
                     /gene="rplD"
                     /locus_tag="ACHKUN_001976"
     CDS             2251..2856
                     /gene="rplD"
                     /locus_tag="ACHKUN_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO6660231.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2853..3155
                     /gene="rplW"
                     /locus_tag="ACHKUN_001977"
     CDS             2853..3155
                     /gene="rplW"
                     /locus_tag="ACHKUN_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO6660232.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3173..3994
                     /gene="rplB"
                     /locus_tag="ACHKUN_001978"
     CDS             3173..3994
                     /gene="rplB"
                     /locus_tag="ACHKUN_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO6660233.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4011..4289
                     /gene="rpsS"
                     /locus_tag="ACHKUN_001979"
     CDS             4011..4289
                     /gene="rpsS"
                     /locus_tag="ACHKUN_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO6660234.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4304..4636
                     /gene="rplV"
                     /locus_tag="ACHKUN_001980"
     CDS             4304..4636
                     /gene="rplV"
                     /locus_tag="ACHKUN_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO6660235.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4654..5355
                     /gene="rpsC"
                     /locus_tag="ACHKUN_001981"
     CDS             4654..5355
                     /gene="rpsC"
                     /locus_tag="ACHKUN_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO6660236.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5368..5778
                     /gene="rplP"
                     /locus_tag="ACHKUN_001982"
     CDS             5368..5778
                     /gene="rplP"
                     /locus_tag="ACHKUN_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO6660237.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5778..5969
                     /gene="rpmC"
                     /locus_tag="ACHKUN_001983"
     CDS             5778..5969
                     /gene="rpmC"
                     /locus_tag="ACHKUN_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO6660238.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5969..6223
                     /gene="rpsQ"
                     /locus_tag="ACHKUN_001984"
     CDS             5969..6223
                     /gene="rpsQ"
                     /locus_tag="ACHKUN_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO6660239.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6388..6759
                     /gene="rplN"
                     /locus_tag="ACHKUN_001985"
     CDS             6388..6759
                     /gene="rplN"
                     /locus_tag="ACHKUN_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO6660240.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6770..7084
                     /gene="rplX"
                     /locus_tag="ACHKUN_001986"
     CDS             6770..7084
                     /gene="rplX"
                     /locus_tag="ACHKUN_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO6660241.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7099..7638
                     /gene="rplE"
                     /locus_tag="ACHKUN_001987"
     CDS             7099..7638
                     /gene="rplE"
                     /locus_tag="ACHKUN_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO6660242.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7653..7958
                     /gene="rpsN"
                     /locus_tag="ACHKUN_001988"
     CDS             7653..7958
                     /gene="rpsN"
                     /locus_tag="ACHKUN_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO6660243.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7992..8384
                     /gene="rpsH"
                     /locus_tag="ACHKUN_001989"
     CDS             7992..8384
                     /gene="rpsH"
                     /locus_tag="ACHKUN_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO6660244.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8397..8930
                     /gene="rplF"
                     /locus_tag="ACHKUN_001990"
     CDS             8397..8930
                     /gene="rplF"
                     /locus_tag="ACHKUN_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO6660245.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8940..9293
                     /gene="rplR"
                     /locus_tag="ACHKUN_001991"
     CDS             8940..9293
                     /gene="rplR"
                     /locus_tag="ACHKUN_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO6660246.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9308..9811
                     /gene="rpsE"
                     /locus_tag="ACHKUN_001992"
     CDS             9308..9811
                     /gene="rpsE"
                     /locus_tag="ACHKUN_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO6660247.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9815..9994
                     /gene="rpmD"
                     /locus_tag="ACHKUN_001993"
     CDS             9815..9994
                     /gene="rpmD"
                     /locus_tag="ACHKUN_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO6660248.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9998..10432
                     /gene="rplO"
                     /locus_tag="ACHKUN_001994"
     CDS             9998..10432
                     /gene="rplO"
                     /locus_tag="ACHKUN_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO6660249.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10440..11771
                     /gene="secY"
                     /locus_tag="ACHKUN_001995"
     CDS             10440..11771
                     /gene="secY"
                     /locus_tag="ACHKUN_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO6660250.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11803..11919
                     /gene="rpmJ"
                     /locus_tag="ACHKUN_001996"
     CDS             11803..11919
                     /gene="rpmJ"
                     /locus_tag="ACHKUN_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO6660251.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12066..12422
                     /gene="rpsM"
                     /locus_tag="ACHKUN_001997"
     CDS             12066..12422
                     /gene="rpsM"
                     /locus_tag="ACHKUN_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO6660252.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12439..12828
                     /gene="rpsK"
                     /locus_tag="ACHKUN_001998"
     CDS             12439..12828
                     /gene="rpsK"
                     /locus_tag="ACHKUN_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO6660253.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12862..13482
                     /gene="rpsD"
                     /locus_tag="ACHKUN_001999"
     CDS             12862..13482
                     /gene="rpsD"
                     /locus_tag="ACHKUN_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO6660254.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13508..14497
                     /gene="rpoA"
                     /locus_tag="ACHKUN_002000"
     CDS             13508..14497
                     /gene="rpoA"
                     /locus_tag="ACHKUN_002000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO6660255.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14538..14921
                     /gene="rplQ"
                     /locus_tag="ACHKUN_002001"
     CDS             14538..14921
                     /gene="rplQ"
                     /locus_tag="ACHKUN_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO6660256.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15028..15396
                     /locus_tag="ACHKUN_002002"
     CDS             15028..15396
                     /locus_tag="ACHKUN_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO6660257.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15407..15832
                     /gene="zntR"
                     /locus_tag="ACHKUN_002003"
     CDS             15407..15832
                     /gene="zntR"
                     /locus_tag="ACHKUN_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO6660258.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15888..16106
                     /gene="arfA"
                     /locus_tag="ACHKUN_002004"
     CDS             15888..16106
                     /gene="arfA"
                     /locus_tag="ACHKUN_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO6660259.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16103..16513)
                     /gene="mscL"
                     /locus_tag="ACHKUN_002005"
     CDS             complement(16103..16513)
                     /gene="mscL"
                     /locus_tag="ACHKUN_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO6660260.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16643..18019)
                     /gene="trkA"
                     /locus_tag="ACHKUN_002006"
     CDS             complement(16643..18019)
                     /gene="trkA"
                     /locus_tag="ACHKUN_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO6660261.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18041..19330)
                     /gene="rsmB"
                     /locus_tag="ACHKUN_002007"
     CDS             complement(18041..19330)
                     /gene="rsmB"
                     /locus_tag="ACHKUN_002007"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO6660262.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(19376..20323)
                     /gene="fmt"
                     /locus_tag="ACHKUN_002008"
     CDS             complement(19376..20323)
                     /gene="fmt"
                     /locus_tag="ACHKUN_002008"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO6660263.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20338..20847)
                     /gene="def"
                     /locus_tag="ACHKUN_002009"
     CDS             complement(20338..20847)
                     /gene="def"
                     /locus_tag="ACHKUN_002009"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO6660264.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20977..22101
                     /gene="dprA"
                     /locus_tag="ACHKUN_002010"
     CDS             20977..22101
                     /gene="dprA"
                     /locus_tag="ACHKUN_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO6660265.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22073..22547
                     /gene="smg"
                     /locus_tag="ACHKUN_002011"
                     /pseudo
     CDS             22073..22547
                     /gene="smg"
                     /locus_tag="ACHKUN_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
     gene            22576..23118
                     /locus_tag="ACHKUN_002012"
     CDS             22576..23118
                     /locus_tag="ACHKUN_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO6660266.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23123..23695
                     /gene="tsaC"
                     /locus_tag="ACHKUN_002013"
     CDS             23123..23695
                     /gene="tsaC"
                     /locus_tag="ACHKUN_002013"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO6660267.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23700..24518
                     /gene="aroE"
                     /locus_tag="ACHKUN_002014"
     CDS             23700..24518
                     /gene="aroE"
                     /locus_tag="ACHKUN_002014"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO6660268.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24515..24772
                     /locus_tag="ACHKUN_002015"
     CDS             24515..24772
                     /locus_tag="ACHKUN_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO6660269.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24748..25302)
                     /locus_tag="ACHKUN_002016"
     CDS             complement(24748..25302)
                     /locus_tag="ACHKUN_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO6660270.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6600 a         6124 c         6679 g         6305 t
ORIGIN      
        1 gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact ggggcctgat
       61 gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg ctgataaata
      121 tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag gcaaactggg
      181 gattggtgaa gatgtcgagg agatgctgca atccgacctg cgattagaac tggaaggcgc
      241 gaaggatctc cgtgaggcta tcgcgtatgc cgacagcgtt catgactatg ttagccgtga
      301 tatgatgatc gagatccttg ccgatgaaga aggtcatatt gactggctcg aaactgagct
      361 ggatctcatc ggtaaaatcg gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga
      421 ttaacggcct gctagcaaac tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg
      481 cccaaacggc agcgggtttt ttaatgactc ttttcctctc atgagcagac caacaactac
      541 ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg
      601 ccaggcacca agggcggcga gaaacttcac atcaccatag cctaatcctt ctttatgacg
      661 cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaataa tcgcccccca
      721 taacgcatcg gctaaaccgt caggattaca aacctgagag aacaaaagac ctgaccagag
      781 taacgggcag gtaaatctgt caggtaacaa gccatgcttt gcatcccata aaaaaagtag
      841 gacagataaa caagcgtaca aaatcagaaa gggaaggtta gcgtccataa aacatcctct
      901 gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagaa atcgctgatt
      961 tgttaacctg gttccggtga aaaacgctat cttcacaacc ttacaaattt cgtggaatac
     1021 agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag gctcagattt
     1081 gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc tcagttagcc
     1141 gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg ttacactctc
     1201 ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa ttggagctct
     1261 ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc
     1321 gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt
     1381 ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc gcacgtcaac
     1441 aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga catcgttgag
     1501 ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg tgtagacgtg
     1561 cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca atgattggtt
     1621 tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt tctatcccag
     1681 taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg gctaacgatg
     1741 gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg accaagcctg
     1801 aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg gaattccgcc
     1861 tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg tttgctgacg
     1921 ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt accgttaagc
     1981 gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac cgcgttccgg
     2041 gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa atggcaggtc
     2101 agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt gacgctgagc
     2161 gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta
     2221 aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga cgcgcagagc
     2281 gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct ggttcaccag
     2341 gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa gactcgtgct
     2401 gaagtaactg gttctggtaa aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct
     2461 ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag
     2521 gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag catcctgtcc
     2581 gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa
     2641 actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct gatcatcacc
     2701 ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc
     2761 gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt aatgactgct
     2821 gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg
     2881 tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa tccaacacca
     2941 tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct gtgcagaaac
     3001 tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc
     3061 acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag
     3121 gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata caatggcagt
     3181 tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga
     3241 gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg
     3301 taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc aggcttaccg
     3361 tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac gtcttgagta
     3421 cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg aacgccgtta
     3481 catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc
     3541 aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta ctgttcataa
     3601 cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta cttacgttca
     3661 gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa
     3721 agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc atatgctgcg
     3781 cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac
     3841 cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact ttggtaagca
     3901 cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca acaagcgtac
     3961 tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc atgccacgtt
     4021 ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag aaagcggtgg
     4081 aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca
     4141 tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta tttgtaaccg
     4201 acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg
     4261 ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa ctatcgctaa
     4321 acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg
     4381 taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag cggctgtact
     4441 ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg gcgctgacat
     4501 tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga agcgcattat
     4561 gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca tcactgtggt
     4621 tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca tcctaatggt
     4681 attcgcctgg gtattgtaaa accatggaac tctacctggt ttgcgaacac caaagaattc
     4741 gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga actggctaaa
     4801 gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt aaccattcac
     4861 actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag
     4921 gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct
     4981 gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg tcgcgttatg
     5041 ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt
     5101 aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga atggtaccgc
     5161 gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac ctctgaagcg
     5221 cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt
     5281 ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt
     5341 aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa
     5401 atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt cggcagcttc
     5461 ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt
     5521 gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa
     5581 ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat
     5641 tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag
     5701 ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac cacctttgta
     5761 actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcgtt gaagagctga
     5821 acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg
     5881 gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga
     5941 ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg
     6001 cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca
     6061 cccgatctac ggtaaattca tcaagcgtac gaccaaactg cacgtacatg acgagaacaa
     6121 cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa
     6181 atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta cactctctcg
     6241 atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc cttgaagcgg
     6301 tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc gggtctcagt
     6361 agtagttgac attagcggag cactaaaatg atccaagaac agactatgct gaacgtcgcc
     6421 gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc
     6481 tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc gcgtggtaag
     6541 gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc
     6601 ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa caacaacagc
     6661 gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg tagtgagaag
     6721 ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca tggcagcgaa
     6781 aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa
     6841 agttaagaat gtcctgtctt ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa
     6901 acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa aagaagccgc
     6961 tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg
     7021 ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg aaactatcaa
     7081 gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga agtagttaaa
     7141 aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt cgagaagatc
     7201 accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga taacgcagca
     7261 gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt
     7321 gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct gcgtggcgaa
     7381 cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat ccgtgacttc
     7441 cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg tgtccgtgag
     7501 cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg tttggatatt
     7561 accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc tgcctttgac
     7621 ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg aaagcacgcg
     7681 aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga
     7741 tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc aagctgcaga
     7801 ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc
     7861 cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc
     7921 gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg
     7981 agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt atccgtaacg
     8041 gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa gtggcaatcg
     8101 ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc gacaccaagc
     8161 ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa agcattcagc
     8221 gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg aaagttatgg
     8281 cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc
     8341 gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag gaaaaaatgt
     8401 ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc
     8461 aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac gatgctgttg
     8521 aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac gcagacggtt
     8581 gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt gttaccgaag
     8641 gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt aaaggcaatg
     8701 tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct gcgggtatca
     8761 ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag caggtgatcg
     8821 gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg
     8881 ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta
     8941 tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc
     9001 tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca caggtaattg
     9061 caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct atcgctgaac
     9121 aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac
     9181 gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc caatatcatg
     9241 gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc taaggtagag
     9301 gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt
     9361 aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag ctctgactgt
     9421 agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg aagttccagc
     9481 agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa
     9541 cggcactctg caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc
     9601 ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc
     9661 tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca acgtggttcg
     9721 tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg
     9781 taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta aaattactca
     9841 aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg gcctgggtct
     9901 gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg gtatgatcaa
     9961 cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata ctctgtctcc
    10021 ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg
    10081 taaaaccggt ggtcgtggtc acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg
    10141 cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc
    10201 tcgtaaagca gcgattacag ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg
    10261 tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga tcgagttcgc
    10321 gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc tgcgtgttac
    10381 taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga
    10441 tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc gagctgaaac
    10501 gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct tttattccga
    10561 tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga ggcaccatca
    10621 ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc tttgctctgg
    10681 ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggta gttcacccaa
    10741 cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc cagtacaccc
    10801 gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc ggtctgccga
    10861 atatgcctgg tatgcaaggc ctggtgatta acccaggctt tgcattctac ttcaccgctg
    10921 ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag attactgaac
    10981 gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc
    11041 cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc
    11101 tgttggttgc agtattagta tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc
    11161 aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac
    11221 agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca atcttcgctt
    11281 ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt actggttgga
    11341 actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat gtgttactct
    11401 atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc aacccgcgtg
    11461 aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt ccgggagagc
    11521 aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cctggttggt gcgctgtata
    11581 ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta ccgttctact
    11641 tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg gctcaagtgc
    11701 aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct
    11761 acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc
    11821 gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt
    11881 tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat atttttcttg
    11941 caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct gtgcgtttcc
    12001 atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa tagtaggagt
    12061 gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc gtaatcgcat
    12121 taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct gcagcgggta
    12181 tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg
    12241 aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc
    12301 gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg
    12361 gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat
    12421 aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa
    12481 caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat cgtgactatc
    12541 actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg tttccgtggt
    12601 tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg
    12661 aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc aggccgcgaa
    12721 tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac tgatgtgact
    12781 ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc
    12841 caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa gctgagccgt
    12901 cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa
    12961 attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg
    13021 cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg tcagttccgt
    13081 aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg
    13141 ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa
    13201 gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt taacatcgct
    13261 tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa gaagcagtct
    13321 cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacctg gctggaagtt
    13381 gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga tctgtctgcg
    13441 gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt accaaagaga
    13501 ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg atatcgagca
    13561 agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct ttggccatac
    13621 tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg cggtgaccga
    13681 ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc aggaagatat
    13741 cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca aagatgaagt
    13801 tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata tcacccacga
    13861 cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc cacctgaccg atgagaacgc
    13921 gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg cttctacccg
    13981 aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg catgctacag
    14041 ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc gtaccgacct
    14101 ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag aggcgattcg
    14161 tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac gtgatgtacg
    14221 tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc gccctgttga
    14281 cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta tccactatat
    14341 cggtgatctg gtacaacgta ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa
    14401 atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct
    14461 ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt taaggtttta
    14521 ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac cgcaacagca
    14581 gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat gaaatcatca
    14641 agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg attactcttg
    14701 ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga
    14761 tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca
    14821 ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct tacatcgagc
    14881 tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca acgtaaaaaa
    14941 acccgccccg gcgggttttt ttatacccgt agcatcccca cttatctaca atagctgtac
    15001 tctttttgtt catcccctgg agtatttatg tggttacttg accagtgggc agagcgtcat
    15061 atagcagaag cgcaagcgaa aggtgagttt gataacctgg caggtagcgg cgaaccattg
    15121 atactggatg atgattctca tgtgccaccg gaattacgtg cggggtatcg cttgctgaag
    15181 aatgccggtt gcttaccgcc agaacttgag cagcggaggg aagcaattca gcttctggat
    15241 atcctgagag gtatccgtca cgatgatccg caatatcaag aggttagccg tcgattgtca
    15301 ttactggaat tgaagctgcg acaagctgga ttgagtaccg attttttacg cggcgattat
    15361 gctgacaagt tgttggacaa aatcaacgat aactagtgga gtatgtatgt atcgcattgg
    15421 tgagctggca aaaatggcgg aagtaacacc tgacacgatt cgttattacg aaaaacagca
    15481 gatgatggag catgaagtgc gtactgaagg cgggtttcgc ctgtataccg aaagcgatct
    15541 ccagcgattg aaatttatcc gccatgccag acaactaggt ttcagtctgg agtcgatccg
    15601 cgagttgctg tcgatccgca tcgatcctga acaccatacc tgtcaggagt caaaaggcat
    15661 tgtgcaggaa agattgcagg aagtcgaagc acggatagcc gagttgcaga gtatgcagcg
    15721 ttccttgcaa cgccttaacg atgcctgttg tgggaccgcc catagcagtg tttattgttc
    15781 gattcttgaa gctcttgaac aaggggcgag tggcgttaag agtggctgtt gattttttgc
    15841 actgacagga ttacactcgc gccgtaaaat aaccaactgg agtttttatg agccgatatc
    15901 agcatactaa agggcagata aaggataatg cgatagaagc attactacat gatcccttat
    15961 tccgacagcg agtagagaaa aataagaaag ggaaaggcag ttacatgcga aaaggaaaac
    16021 atggcaatcg ggggaactgg gaggccagtg gcaagaaagt aaatcacttt tttaccactg
    16081 gtcttctgct ttcaggcgct tgttaagagc ggttattctg ctctttcagc aaatcacgaa
    16141 tttctgccag taatacttct tctttagttg gtgcaggtgc ggcagctggt tcttcttttt
    16201 tccgattcag tttgttgatt agcttaatcg ccataaagat ggcaaaggcc acaatcagaa
    16261 aatcaaagac gttttgaatg aagacaccgt aatgcatcac aacagcaggg atatccccct
    16321 gcgcatcgcg tagcgtgaca gcaaactgtt taaaatcgat cccgccaatt aataagccca
    16381 gaggaggcat gatgatatcg gcaaccagtg aagagacaat cttcccgaat gccgcaccga
    16441 taatgacacc caccgccaaa tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt
    16501 taataatgct catgttattc tccctatgaa gccgacaacc ataagtctaa caaatgttaa
    16561 gccattttcc tgccaggaat gaatttaatt aatccttgat aatcaaaggg aaataataag
    16621 gcgtcattag acgccttatt aattacaaga agaaagggct tggttggaag agtctttcga
    16681 cgtcggtaat aaacttttta tctgtgagga acataattac gtgatcgcct tgctcaatgc
    16741 gcagattgtc attggcaatc atcacgtcgt ttccacgtac caccgctcca ataatcgttc
    16801 ctggcggtag cttgatttcg tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac
    16861 cgtgagcaac ggcttcaata gcttctgcta cgccgcggcg caatgaggaa acaccaacaa
    16921 tatctgcttt tcgcacatgg ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg
    16981 caatatcgat cacactcccc tgaaccagat ccacataagc gcgacgctgg atcaatacca
    17041 tcaccttttt cgcacccata cgtttggcaa gcatggcgga catgatattg gcctcgtcat
    17101 cgttggtgac ggcaataaac agatcaactt gatcgatatg ttcttcggcc agcagttctt
    17161 gatccgacgc atcaccaaaa aagacgatcg tattctgcaa cttttccgcc agttcggcag
    17221 cgcgctgctg attacgttcg atgagtttaa cgctgtaatc tttttccaga cgacgcgcca
    17281 gccctgcacc gatattaccg ccaccaacca gcatgatccg cttatacggt ttttccagtc
    17341 gctgtaattc actcatcacc gcacggatat gctgtgaagc ggcaataaag aacacttcat
    17401 caccagcttc cacaatggtc gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg
    17461 ctgccacacg agtatcgata tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa
    17521 tcagcgggcc gccataatag gctttaacca cagcgaggct gactttaccc tcagcgaagt
    17581 tcaccacctg caatgcgccg ggatactcaa tcagtcggta aatattatcg ataaccaact
    17641 gctctggtgc gatcagatga tcaatcggta cagcatctga atgaaatagc ttatcggcat
    17701 cgcgcacgta gtctggtgag cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt
    17761 aggctacctg gcaggcaacc atattggttt catctgaact ggttacagca accagcatat
    17821 cggcgtcgtc ggcacctgcc tcccgcaata cgcgtggatg agagccatgc ccctgcacga
    17881 ctcgcaggtc aaatttatcc tgcaaggtcc gcagacgctc accgttggta tcgacaacag
    17941 tgatatcgtt gttctcgcca accaggtttt ccgccagtgt gccgccaacc tggccggcac
    18001 ccagaatgat aattttcatc agtcgcgacc cgttatctca tcactttttg attagcttag
    18061 cgtaaaagaa gccgtcgccc tcttcggtac caggcagatt ttgtttaccc ggttgctctg
    18121 gtgttcctgt ttcgcaaagt tcggcatcag cggtacgttg caaaaaggct ttaatctgca
    18181 ggctattctc ttccggtaac accgaacagg tggcatagac cagggttcca ccggatttta
    18241 aatgcggcca aatggcgtcg agaatttcag actgcaattg cgcgagttcc gggatatcgc
    18301 gatcgcgacg taaccattta atatccggat ggcgacgaat cacaccggtt gctgaacaag
    18361 gcgcatctaa taaaatgcga tcaaactgtt gctcgccaca ccattgggaa gggtagcggc
    18421 catcaccttg tttcacggtc gctttcatac caaggcgttt taaattgtcg taaacgcgag
    18481 agaggcgctg ttcgtcaata tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga
    18541 tatgcgttgt tttaccgccg ggggcggcac aaagatccag aatgtgttca ccgttttgtg
    18601 gcgcaagcca ggtcatgcaa ccttgtgctg atgcatcctg aacggtaacc catccgtctt
    18661 caaaaccagg tagcgcatga acaggtgcag gtgtttccag ccgtacagca tcagggtaat
    18721 ccgcatgcgg gaaacctttc attcctgctt catccaacaa tgcaagccag ctgtcgcggg
    18781 aatgatgcgt acgattaacg cgcagccaca ttggcggacg ctggttatta gcttcgacga
    18841 tggattgcca ctgctctgga tacgcttttt gcagacgctt cagcaaccag gaaggatgca
    18901 gataacgtgc atcactggca taaaacccgg ctaataactc ttcttgctga cgctggaact
    18961 ggcgtaatac accgttaatc agccccttaa gttgcgggcg cttaattgcg acagcgcctt
    19021 caaccgtttc agccagcgca gcatgaggtg gaatgcgggt ataaagcagt tgatacacac
    19081 caaccataat caggtaatgc acggtccgct gtttgccggt catcggacgg gccattaact
    19141 tattaatcag ccagtctaac tgtgaaagcg tacgcagtac gccaaagcac aactcttgaa
    19201 gaagtgcttt gtctttatcg gaaacttttt gctggagcgg tggcagaatg ttgcttaatg
    19261 attgcccttg ctcgacgatt tgttcaacgg cctgtgccgc catgctacgt aaattacgtt
    19321 gttttttcat aagtataaaa atgcccggta agaccgggct tagaagagtg gactatcaga
    19381 ccagacggtt gcccggaaca aaccattccc gacgagaatt caggaggtct tgcgcgctca
    19441 tcgctttctt acccgcaggt tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa
    19501 cctgaatgcc ttgtttattg gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat
    19561 caatgaccga cgctttccag actttaaccg gttgtccttc aatttccagc cagctcattg
    19621 gccatgggtt gaaagcgcga atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa
    19681 tacgcgcttc ttctttactc aacttctcgg cgtaagtgac aagagtttcg tcctgaactt
    19741 ctggtttcgc cgtgccgtct gctagttgtt tcaacgtggt gataagccct tgtgggccaa
    19801 gctctgccag cttgtcgtac agcgtaccac tggtatcttc tgcagtaatc gggcaggaga
    19861 gcttataaag catgtcaccg gtgtctaaac caacatccat ttgcataatg gtcacaccag
    19921 tttctgcatc acccgcccat agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca
    19981 gcagtgaacc atgaacgttg atacagccaa gacgcggcat ctccagcact gctttaggca
    20041 gaattaagcc ataggcgacg acgaccataa catcagcctg cagatcggcg accagttgct
    20101 ggctttcttg tggacgcagg gaaacaggtt gaaaaacggg cagacctttt tcctcagcca
    20161 gaactttaac cgggctgggc atcagttttt taccgcgtcc tgccggtcgg tctggctggg
    20221 tgaacacgcc aacgacgtta tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa
    20281 agtcaggtgt acccgcaaaa ataatacgta gtgattctga cacgttagtt cttatcctta
    20341 agcccgggct ttcagacgat ctagtttttc aactttctga cgaatacgtt gttgtttcag
    20401 cggtgacaga taatccataa acagtttgcc gaccaggtga tccatctcat gctgaataca
    20461 gatggctaac agcccctctg cttccagttc aaatggttta ccgtcgcggt caagggcgcg
    20521 aattttaact ttctctgcgc gcggcactaa agcacgttgt tcagggatcg acaggcaacc
    20581 ttcttcaatg cctgtttcgc cgcttttttc taaaagctcc ggattgatta acaccagccg
    20641 ttcgtcacgg ttttccgaaa catcaataac aatgatacgt tgatggatat caacctgggt
    20701 tgccgccagg ccaatacctt cttctgcgta catcgtctcg aacatatcat cgacgatacg
    20761 ctgtatttct gcattcactt cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg
    20821 aatatgtaac acttgcaaaa ctgacataaa tctccagaga tgtgttcagg agttagaaag
    20881 attatttctt ctattctaga caaatccccc tctgattgac agcatcactg accaatcgca
    20941 aagattgcta aggctgctta tggcagggag ataaggatgg tcgatacaga aatttggctg
    21001 cgtttaatga gtatcagcag cttgtacggc gatgatatgg tccgtatagc tcactggctg
    21061 gcaaaacagt cgcaaattga tgcggttgga ttgcagcaaa cagggcttac attgcggcag
    21121 gcacaacgct ttctttcatt tccgcgaaag agtatcgaaa gctcactttg ttggttggag
    21181 caacccaacc atcatttaat ccctgcggac agcgaatttt atcctcctca acttctggcg
    21241 acgacagatt accccggcgc actgtttgtt gaaggagaac tgcacgcgct gcattcattt
    21301 cagcttgccg tagtggggag tcgggcgcat tcatggtatg gcgagcgatg gggacggtta
    21361 ttttgcgaaa ctctggcgac gcgtggagtg acaattacga gtggactggc gcgtggaatc
    21421 gatggtgtgg cgcataaagc ggccttacag gtaaatggcg tcagcattgc tgtattgggg
    21481 aatggactta ataccattca tccccgccgc catgcccgac tggctgccag tctgcttgaa
    21541 catggcggag ctctcgtctc ggaatttccc ctcgatgttc caccccttgc ttacaatttc
    21601 ccacgaagaa atcgcattat cagtggtcta agtaaaggtg tactggtggt ggaagcggct
    21661 ttgcgcagtg gttcgctggt gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt
    21721 gccttgccag gtccaatagg gaatccggga agcgaagggc ctcactggtt aataaaacaa
    21781 ggtgcgattc ttgtgacgga accggaagaa attctggaaa acttgcaatt tggattgcac
    21841 tggttgccag acgcccctga aaattcattt tattcaccag atcagcaaga cgtggcattg
    21901 ccatttcctg agctcctggc taacgtagga gatgaggtaa cacctgttga cgtcgtcgct
    21961 gaacgtgccg gccaacctgt gccagaggta gttactcaac tactcgaact ggagttagca
    22021 ggatggatcg cagctgtacc cggcggctat gtccgattga ggagggcatg ccatgttcga
    22081 cgtactaatg tatttgtttg aaacctatat tcacacagaa gctgagttgc gtgtggatca
    22141 agacaaactt gaacaggatc ttaccgacgc aggatttgat cgagaagata tctacaatgc
    22201 cctgctttgg ctggaaaagc ttgcggatta tcaggaagga ctggcagaac cgatgcaact
    22261 ggcctctgat ccactctcca atgcgtattt atacccctga ggagtgtgaa agactggatg
    22321 ccagctgtcg tggttttctg cttttccttg agcaaattca ggtgcttaac cttgaaaccc
    22381 gtgaaatggt gatagagcga gtgctcgcgc tggataccgc agagttcgac ctggaagacc
    22441 tgaaatgggt aatcctgatg gtgttgttca atattccggg ctgcgaaaat gcgtaccagc
    22501 aaatggaaga attactcttt gaagtgaatg aaggtatgct gcattaatca tttctttaat
    22561 tcagcataag ttgttatggc gaaatcagca ctgttcacgg tgcgtaataa tgagtcctgc
    22621 ccaaagtgcg gggctgaact ggttattcga tccgggaaac acggtccgtt tcttggatgc
    22681 tcacagtatc cggcgtgtga ctacgtccgt cctctgaaat cttcagcgga tggacatatt
    22741 gtcaaagttc tggaggggca ggtttgcctt gcatgtggcg caaatctggt attacgccag
    22801 ggacgctttg gtatgtttat tggttgcagt aactaccctg aatgcgaaca taccgaactt
    22861 atcgataaac cggacgaaac agcaattaca tgcccccaat gccggacggg ccatctggtc
    22921 cagcgccgct cccgttatgg caaaacattt cactcctgtg atcgctaccc ggagtgtcaa
    22981 tttgccatta acttcaaacc catagctgga gaatgccctg agtgtcatta tccactactc
    23041 atcgaaaaga aaaccgcgca gggtgtaaaa cacttttgtg ccagtaaaca atgtggaaag
    23101 ccggtttcgg cggaataata acgtgaataa taacctgcaa ggagacgcta tcgcagctgc
    23161 gatagatgtc ctcaatgaag aacgtgtcat cgcctatcca acggaagccg ttttcggtgt
    23221 tgggtgcgat cctgatagcg aaacagcagt gatgagactg ttggagttaa aacagcgtcc
    23281 ggttgataag gggctgattt taatcgcggc aaattacgag cagcttaaac cctatattga
    23341 tgacaccatg ctgactgatg cgcagcgtga aaccattttt tcccgctggc caggtcctgt
    23401 cacctttgtc tttcccgcgc ctgcgacaac accgcgctgg ttgacgggcc gctttgattc
    23461 gcttgctgta cgagtcaccg accatccgtt ggtggttgct ttgtgccagg cttatggtaa
    23521 accgctggtt tctaccagtg ccaacttgag tggattgcca ccttgtcgaa cagtagacga
    23581 agttcgcgca caatttggcg cggcgttccc ggttgtgcct ggtgaaacgg gggggcgttt
    23641 aaatccttca gaaatccgcg atgccctgac gggtgaactg tttcgacagg ggtaacataa
    23701 tggaaaccta tgctgttttt ggtaatccga tagcccacag caaatcgcca ttcattcatc
    23761 agcaatttgc tcagcaactg aatattgaac atccctatgg gcgcgtgttg gcacccatca
    23821 atgatttcat caacacactg aacgctttct ttcgtgctgg tggtaaaggt gcgaatgtga
    23881 cggtgccttt taaagaagag gcttttgcca gagcggatga gcttactgaa cgggcagcgt
    23941 tggctggtgc tgttaatacc ctcaagcggt tagaagatgg acgcctgctg ggtgacaata
    24001 ccgatggtat aggtttgtta agcgatctgg aacgtctgtc ttttatccgt cctggtttac
    24061 gtattctgct catcggcgct ggtggagcat ctcgcggcgt actactgcca cttctttccc
    24121 tggactgtgc ggtgacaatc actaatcgga cggtatcccg cgcggaagag ttggctaaat
    24181 tgtttgcgca cactggcagt attcaggcgt tgggtatgga cgaactggaa ggtcatgagt
    24241 ttgatctcat tattaatgca acatccagtg gcatcagtgg tgatattccg gcgatcccgt
    24301 catcgctcat tcatccaggc atttattgct atgacatgtt ctatcagaaa ggaaaaactc
    24361 cttttctggc atggtgtgag cagcgaggct caaagcgtaa tgctgatggt ttaggaatgc
    24421 tggtggcaca ggcggctcat gcctttcttc tctggcacgg tgttctgcct gacgtagaac
    24481 cagttataaa gcaattgcag gaggaattgt ccgcgtgaat caggccatcc agtttccgga
    24541 cagagaagag tgggacgaga ataaaaaatg tgtatgtttt cccgctctcg tgaatggcat
    24601 gcaactgaca tgtgcgatct ctggcgagag tctggcgtat cgttttactg gagatacgcc
    24661 agaacagtgg ttagcgagtt ttcgtcagca tcgctgggat ctggaagaag aagcggaaaa
    24721 cttaattcag gaacaaagtg aagatgatca aggctgggtc tggttaccct gatccagata
    24781 ttcgtccttc catttcacgt aattattcgc ggaatagcgt aacccagcct tctcttcatc
    24841 acttaacggg cggatctgtt tgacggggct accgagatac agatatccgc tctccagccg
    24901 tttattttgt gggaccagac tacccgcacc aatcatcaca tcatcttcta ctattgcgcc
    24961 atcaagtaaa attgagccca tcccaaccaa aactcgattg ccaatggtgc agccgtggag
    25021 catcaccttg tgaccaacag tgacatcttc gccaatggtt aatgggttgc catctgggtt
    25081 gtacgaggat ttatgagtga catgcaacat actgccatcc tggatattgg tgcgtgctcc
    25141 gatctgtaca taatgtacat ctccacgaat cacaacgagc ggccagatcc ccacatcatc
    25201 agccagacga acgtcaccaa tcacgacact gctatcgtcg atcattacgc gctgaccgat
    25261 ttgtggaaaa agatcgcggt atgggcgtaa aacatcagac atacttacct cagcaataaa
    25321 tgatttacta atgactttgg gggcattatt ggccttgtgc aagtctttta gtatgcaaaa
    25381 aagcaccgtt ttgtgtgcga atgcagcaaa aagggtgaaa aaacaacaaa cagaaaaaaa
    25441 gatcaaaaaa gtacttgtgc aaaaaattgg gatccctata atgcgcctcc gttgagacga
    25501 caacgtgaaa cacttcacaa gatggtcgta acaacgaaga gaaaaaatcc tgaaattaag
    25561 ggttgactct gaaagaggaa agcgtaatat acgccacctc gcgacagtga gctgaaagcc
    25621 gcgtcgcaac tgctctttaa caatttatca gacaatctgt gtgggcactc gaagatacgg
    25681 attcttaacg tcgcaagacg aaaaatga
//
LOCUS       DAZFRJ010000054        25513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000054 DAZFRJ010000000
VERSION     DAZFRJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.054"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            306..725
                     /gene="umuD"
                     /locus_tag="ACHKUN_002017"
     CDS             306..725
                     /gene="umuD"
                     /locus_tag="ACHKUN_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6660271.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            725..1993
                     /gene="umuC"
                     /locus_tag="ACHKUN_002018"
     CDS             725..1993
                     /gene="umuC"
                     /locus_tag="ACHKUN_002018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO6660272.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(2039..2569)
                     /gene="dsbB"
                     /locus_tag="ACHKUN_002019"
     CDS             complement(2039..2569)
                     /gene="dsbB"
                     /locus_tag="ACHKUN_002019"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO6660273.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2715..4256)
                     /gene="nhaB"
                     /locus_tag="ACHKUN_002020"
     CDS             complement(2715..4256)
                     /gene="nhaB"
                     /locus_tag="ACHKUN_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO6660274.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4478..5197
                     /gene="fadR"
                     /locus_tag="ACHKUN_002021"
     CDS             4478..5197
                     /gene="fadR"
                     /locus_tag="ACHKUN_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO6660275.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5249..6781)
                     /gene="ycgB"
                     /locus_tag="ACHKUN_002022"
     CDS             complement(5249..6781)
                     /gene="ycgB"
                     /locus_tag="ACHKUN_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO6660276.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7111..8409
                     /gene="dadA"
                     /locus_tag="ACHKUN_002023"
     CDS             7111..8409
                     /gene="dadA"
                     /locus_tag="ACHKUN_002023"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO6660277.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVHPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8419..9489
                     /gene="dadX"
                     /locus_tag="ACHKUN_002024"
     CDS             8419..9489
                     /gene="dadX"
                     /locus_tag="ACHKUN_002024"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO6660278.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9875..11611)
                     /gene="cvrA"
                     /locus_tag="ACHKUN_002025"
     CDS             complement(9875..11611)
                     /gene="cvrA"
                     /locus_tag="ACHKUN_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO6660279.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11706..12620)
                     /gene="ldcA"
                     /locus_tag="ACHKUN_002026"
     CDS             complement(11706..12620)
                     /gene="ldcA"
                     /locus_tag="ACHKUN_002026"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO6660280.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12720..13331
                     /gene="emtA"
                     /locus_tag="ACHKUN_002027"
     CDS             12720..13331
                     /gene="emtA"
                     /locus_tag="ACHKUN_002027"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO6660281.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13333..14067)
                     /gene="ycgR"
                     /locus_tag="ACHKUN_002028"
     CDS             complement(13333..14067)
                     /gene="ycgR"
                     /locus_tag="ACHKUN_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO6660282.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14268..14522
                     /gene="ymgE"
                     /locus_tag="ACHKUN_002029"
     CDS             14268..14522
                     /gene="ymgE"
                     /locus_tag="ACHKUN_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6660283.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14700..15140
                     /gene="ycgY"
                     /locus_tag="ACHKUN_002030"
     CDS             14700..15140
                     /gene="ycgY"
                     /locus_tag="ACHKUN_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO6660284.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15219..16916)
                     /gene="treA"
                     /locus_tag="ACHKUN_002031"
     CDS             complement(15219..16916)
                     /gene="treA"
                     /locus_tag="ACHKUN_002031"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6660285.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17236..18654)
                     /gene="dhaM"
                     /locus_tag="ACHKUN_002032"
     CDS             complement(17236..18654)
                     /gene="dhaM"
                     /locus_tag="ACHKUN_002032"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO6660286.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18665..19297)
                     /gene="dhaL"
                     /locus_tag="ACHKUN_002033"
     CDS             complement(18665..19297)
                     /gene="dhaL"
                     /locus_tag="ACHKUN_002033"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO6660287.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19308..20378)
                     /gene="dhaK"
                     /locus_tag="ACHKUN_002034"
     CDS             complement(19308..20378)
                     /gene="dhaK"
                     /locus_tag="ACHKUN_002034"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO6660288.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20606..22525
                     /gene="dhaR"
                     /locus_tag="ACHKUN_002035"
     CDS             20606..22525
                     /gene="dhaR"
                     /locus_tag="ACHKUN_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO6660289.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22625..25510)
                     /gene="ycgV"
                     /locus_tag="ACHKUN_002036"
     CDS             complement(22625..25510)
                     /gene="ycgV"
                     /locus_tag="ACHKUN_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6660290.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         6295 a         6343 c         6589 g         6286 t
ORIGIN      
        1 acttcatcta attcatagtt agccgggcgg gatgcgtcaa tgtctttatt tctattaata
       61 tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc tattttttag
      121 ccgggtaata tttttcattt ctgctggatg agcgtagtcg ccagaaggcc gcgtgagcac
      181 aggataagag aacgaaaaat cagcagtcta tgcagcgaca aatattgata gcctgaatca
      241 gtattgatct gctggcaaga acagactact gtatataaaa acagtatagc ttcaggcaga
      301 ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt ccgctattta
      361 gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgttgaa cagcgcatcg
      421 atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa gcaagtggtg
      481 attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat agcgctatta
      541 ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg gtgaaaaaat
      601 tgcaactacg cccgacggta cagcttattc ccatgaacag tgcgtactcg cccattacca
      661 tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt aaggcgatgc
      721 gctgatgttt gctctctgtg atgtaaacgc gttttatgcc agctgtgaga cggtgtttcg
      781 ccctgattta tggggtaaac cggtggttgt gctatcgaat aatgacggtt gcgttatcgc
      841 ccgaaacgct gaggcaaagg cgctgggtgt taaaatgggc gatccctggt tcaaacaaaa
      901 agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc tttacgcaga
      961 catgagcaat cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg agatttacag
     1021 tattgatgag gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc tgactgattt
     1081 tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg gtgtggggat
     1141 cgcccagacc aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat ggcagcggca
     1201 gacggggggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa tgtctgctct
     1261 ccccgtggat gaagtctggg ggattggacg gcggatcagc aaaaaactgg acgcgatggg
     1321 gatcaaaacc gttcttgatt tggcggatac agatatccgg tttatccgta aacattttaa
     1381 tgtcgtgctc gaaagaacgg tgcgtgaact gcgcggcgaa ccctgtttgc aactggaaga
     1441 gtttgcaccg acgaagcagg aaattatctg ttcccgctcg tttggtgaac gcatcacgga
     1501 ttatccatcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg aaaaacttcg
     1561 cagcgagcat caatattgtc ggtttatctc cacatttatt aagacgtcac catttgcgct
     1621 caatgaacct tattacggca atagcgcgtc ggtaaaactg ctgacgccca ctcaggacag
     1681 cagggatatc attaacgctg ctacgcgatc tctggatgcc atctggcaag cgggccatcg
     1741 ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt cagggagtcg cgcagctcaa
     1801 tttattcgat gacaacgcac cgcgccccgg gagtgagcaa ttgatgacgg taatggatac
     1861 actgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga tccagcaaca
     1921 atggcagatg aagcgtgcca tgctttcacc acgttataca acgcgaagtt ctgatttact
     1981 gcgggtcaaa taaatatagc ggcaggaaaa aagcgctccc gcaggagcgc cgaatggatt
     2041 agcgaccgaa caggtcacgt tttttcgctt taaacggctg ggaaatcacc accagcaccg
     2101 cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc atacctaaaa
     2161 aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc acccatttat
     2221 ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaac ggcgaaggat
     2281 agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca ctatacaacc
     2341 agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca atcagcgcag
     2401 cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa ggtttcagta
     2461 acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta aacgccatca
     2521 acaaccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata atcatttccc
     2581 tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac caggaggcag
     2641 caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc atttatatgc
     2701 ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca ttcggttaca
     2761 ggggcaagcg taaactcgac gcagagcaag ccgacgagtg tcagtacgag ggtgtaaggc
     2821 agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag tgcagaggtc
     2881 agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cggagggcag attggtaccg
     2941 gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc actttccatt
     3001 gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga aatggatgac
     3061 agcagaccgt tgaaaatata gaacagcgac agctgagcat gttccgatgc ctgcaacaca
     3121 aactgaataa ttggcgaaaa cagttgttgg tcgataatca ccgcgactac cgagaaaaac
     3181 accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat agcatgttca
     3241 tcggtgacac cggtcaatga tgtcgccaga ataatgactg acaaaccaat caagccaact
     3301 tccgccagat gcaacgccag cgcagtcacc agccagacgc caataatcgc ctggacaatc
     3361 agacggattt tatcctgacg agtacgctgg tggcggcttt gatcgtcaaa ctgttgcaac
     3421 acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag cttctctacc
     3481 agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga catgcgcagg
     3541 aagaaatcgc caaaatgcca gccagccgct ttagcgatga tcaggttctg tggttcgccc
     3601 accatggtca tcacgccgcc taatgcagtg ccgacaccgg catgcatcat caggctacgc
     3661 agaaagccac ggaactgttc cagaaccact ttgtaatgct tgtcgatatg actatcgtct
     3721 tgcaggtcgg tatcttcagt acgggaagag gctacgcggt gataaatacc ataaaaaccg
     3781 actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc ggagaggaac
     3841 gccgccgcca cgcaaaaaga gagcgacagc agcattttgg agcgaatgct taacagcaaa
     3901 cgggtaaata tgaacagcaa cagctgtttc ataaaataga tacccgccac cataaacatc
     3961 aataacagca agacttcaag atttgccgcc acctcttcac ggacgtgttc cgcgctggtc
     4021 atgccgatga ataccgcttc gatagccaac agaccaccgg ggagcagcgg gtagcatttc
     4081 agggccatcg ccagagtgaa aataaattcc gctaccagca accagcccgc gacgaaaggg
     4141 ctgatgagga aaattaacgg gtttacgatt aagaaaatga tgagggcgag tttgtaccag
     4201 tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat ctccatgatg
     4261 gtttccctta ccttacaaat aatcaatgat gtttttatgt ttaaacgcaa agcttaacgg
     4321 tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga aaatgaagcc
     4381 ttgatccctt ttttcttctt tttgtctgct atcagcgtag ttagccctct ggtatgatga
     4441 gtccaacttt gttttgctgt gttatggaaa tctcactatg gtcattaagg cgcaaagccc
     4501 ggcgggtttc gcggaagagt acattattga aagtatctgg aataaccgct tccctcccgg
     4561 gactattttg cccgcagaac gtgaactttc agaattaatt ggcgtaacgc gtactacgtt
     4621 acgtgaagtg ttacagcgtc tggcacgaga tggctggttg accattcaac atggcaagcc
     4681 gacgaaggtg aataatttct gggaaacctc tggtttaaat atccttgaaa cactggcgcg
     4741 actggatcac gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc gtaccaatat
     4801 ttccactatt tttattcgca ccgcgtttcg tcagcatccc gataaagcgc aggaagtgct
     4861 ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg attacaacat
     4921 attccgcggc ctggcgtttg cttccggcaa cccgatttac ggtctgatcc ttaacgggat
     4981 gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc aatccggaag cgcgcagtct
     5041 ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc acgatcaggt
     5101 gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag atttggcacc ggatgcagaa
     5161 aaatctgccg ggtgatttag ccattcaggg gcgataatcc cttccgttta aagagcaaac
     5221 ccctcaaacg aggggttttt tgttgttttt acagatttcc cattcttggc gggcaacgtt
     5281 ccagcaactc gatgctgccg tcttcgtttt gctgttcgag catcacatca aatccccaca
     5341 ggcgatgcac atgcttcagc acttctttgc gcccccgatc cagcggtgcg cgattatgtg
     5401 gaatgtaacg cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag atctgaatat
     5461 tcggctccag attacttaag ttatattgcg acgataaccg gttacggatc tcccgataac
     5521 cttcttcatt atgaatagcg gaaatctcca gataattatg ccgatcatca tccagcacgg
     5581 tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa ctggctgata aaactctcat
     5641 ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg gcgatatccg
     5701 ggaaccagta tttgtcttct tccgttggcg actgacaaat ccgtttaata tcctggaaca
     5761 tggcgaaccc gagggcatac gggttgatgc cgctgtacca cgggctgtta taggggggct
     5821 ggaagaccac attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc gttactttcc
     5881 cttcatcata cagatggtta aggatggtat agtgccagaa ggtcgcccag ccttcgttca
     5941 tcacctgagt ctgtttttgc ggataaaaat actggctcac cttacgcaca atacgcagga
     6001 tttcacgctg ccatgattcc agcagtggcg catttttctc cataaaatag agcaggtttt
     6061 cttgtggttc ggacggatag cggcgcgctt cagcaaccgt tttctcttcc tcgcgcttcg
     6121 gcagggtacg ccagagcata ttgacctgac tttgtagata ctcttcgcga cttttctgcc
     6181 gggctttctc ttcttgcagt gagatttttt gcgggcgttt gtaccggtcc acgccgtagt
     6241 tcatcagcgc gtggcacgag tccagaagcc gttctacttc atcaacgcca taacgttctt
     6301 cgcactcggt aatatattta cgggcaaaaa tcagataatc gacaatcgaa ctggcgtcgg
     6361 tccaactgcg gaataagtaa ttgtttttaa agaaagagtt atgcccatag caggcgtgtg
     6421 ccatcaccag cgcctgcatg gtaatggtgt tctcttccat caggtaagcg atacacgggt
     6481 tagagttaat gacgatttca taggccagtc cttgctgacc gtgcttatac agccgttcag
     6541 tttcgataaa ctttttaccg aatgaccagt gcggatagtt aattggcatg ccgacgctgg
     6601 agtaggcatc catcatctgt tctgaggtta tcacttcaat ctggtgcggg taggtatcca
     6661 gccggtagag tttcgccacc cggtctatct ctgccagata aacatccagc aggtcgaacg
     6721 tccagtcggg tccatcgctc aaacgtgtgg tgtccttatt catagaatcg atcgtcgcca
     6781 tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc acctcatttc aagcatagaa
     6841 cacctgttaa aaaccgcgtc gccggagaat ttttttcttt gcgatttctt attatcagag
     6901 tgccactaat ccgcttctga acggaatttt atgctggata aaaagggcgt tcagcaggag
     6961 atactaaaga cgccatattg ccgcagagtc agggagatgt gagccagctc accataaaaa
     7021 agccgtatgt tgaataatat tttcaactga gttatcaaga tgtaattaga ttattattct
     7081 tttactgtat ctaccgttat cggagtggct atgcgagttg tcatactggg aagtggtgtg
     7141 gtaggcgttg ccagcgcctg gtacttaaat caggcaggac atgaggtcac cgtcattgat
     7201 cgggagccgg gggccgccct ggaaaccagc gccgcgaatg ccgggcaaat ctcccccgga
     7261 tatgctgcac cgtgggcggc accaggtgtg cctttaaaag cgatcaaatg gatgttccag
     7321 cgccatgcgc cgctggcggt ccgtctcgac ggtacgcagt ttcagttgaa atggatgtgg
     7381 caaatgttac gtaactgcga caccagccac tacatggaaa acaaagggcg gatggtgcgt
     7441 ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa tattcagtat
     7501 gaagggcgtc agggtgggac gctgcaactg ttccgtaccg aacaacagta tgaaaatgcg
     7561 acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct ggaatccagc
     7621 cgcctggcgg aagtggagcc cgcgctggca gaagtagcgc acaaactgac gggtggtctg
     7681 caactgccca atgatgaaac cggagactgc cagctgttta cccagaatct ggcgcggatg
     7741 gcagagcagg cgggggttaa attccgcttt aatacgcccg ttgaccaact gctttgcgac
     7801 ggcgagcaaa tctacggcgt gaagtgtggc gatgaagtga ttaaggccga tgcgtatgtg
     7861 atggcgtttg gttcttactc gacggcgatg ctcaaaggca ttgttgatat tccggtgcat
     7921 ccgctgaaag gctactcgct gactattcca attgcgcagg aagatggtgc gccagtatcc
     7981 actattcttg atgaaaccta caaaatcgcc attacccgtt tcgataaccg cattcgtgtt
     8041 ggcggaatgg cggaaattgt tggttttaac accgagctgt tgcaaccgcg tcgtgaaacg
     8101 ctggaaatgg tggttcgcga tctctatcca cgcggcggtc atgtcgagca ggcgactttc
     8161 tggacgggtt tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg tacacgcttt
     8221 aaaaatctgt ggctgaatac cggtcacggc acgctcggct ggacgatggc ttgcggttcc
     8281 ggtcagttgt taagcgatct gctctctggt cgcacgccag cgatcccata tgaggatcta
     8341 agcgtagcgc gctacagccg tggatttacg ccatcacgtc cgggccattt acatggcgca
     8401 cacagctaag gaaacgagat gacccgtccg atacaggcca gcctcgatct gcaggcatta
     8461 aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg cgcgcgtctg gtcggtggta
     8521 aaagcgaacg cttacgggca tggtattgag cgtatctgga gcgcgctcgg ggccaccgat
     8581 ggctttgcat tacttaacct ggaagaggca ataacgttac gtgagcgcgg ctggaagggg
     8641 ccgatcctga tgctggaagg atttttccat gctcaggatc tggagattta tgaccagcac
     8701 cgcctgacca cctgcgtaca cagcaactgg cagctcaaag cactgcaaaa tgcgcggcta
     8761 aaagcaccgt tggatattta tcttaaagtg aacagtggga tgaatcggtt gggcttccag
     8821 cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa tggcgaatgt tggcgagatg
     8881 accctgatgt cgcattttgc cgaagcggaa catcctgatg gaatttccag cgcgatggcg
     8941 cgtattgagc aggcggcgga agggctggag tgtcggcgtt cgttgtccaa ttcggcggcg
     9001 actctgtggc acccggaagc gcattttgac tgggttcggc ctggcattat tttgtatggc
     9061 gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg gattacgtcc ggtgatgacg
     9121 ctaagcagtg agattattgg tgtccagacg ctaaaagcgg gtgagcgtgt gggctacggc
     9181 ggtcgctata ctgcgcgcga tgaacagcga atcggcattg tcgccgcagg gtacgccgac
     9241 ggttatccgc gccacgcgcc taccggtacc cctgttttag tggacggcgt gcgcaccatg
     9301 acggtgggga ccgtctcgat ggatatgcta gcggtcgatt taacgccttg cccgcaggcg
     9361 gggattggta cgccggttga gctgtggggc aaggagatca aaattgatga tgtcgccgcc
     9421 gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg cgttacgcgt cccggttgtg
     9481 acagtgtaac ttgttgtaag ccggatcgga ggcagcgtct tctggttgcg aaaaaatcat
     9541 ccatccggct ggtcagcaac tgtagttgtt aatgtgacag agccattgcc catgatagtg
     9601 tccattaaaa gaatggacac tatttccccg gaacctgaac tcaccgcaca ggcgttctac
     9661 ataaaacgct tacgcttcat tgttgactcg aactcgactt cagataaatc acgctttacc
     9721 cttgatggag cctgtacata gatttgtgta attgcctgat tttgatatgt tcaattcaac
     9781 atcaaatgaa ggttaaatta tggacgacaa acaattgcag gctcaggctg cgttcagcaa
     9841 agcatcgcaa ccggcgatag atgcttcatt aaatttaaga ttcagcttcc tcttcagcca
     9901 cccgtacgcc aatcttcaac acttcattat cttctttctc ggccaccgtc cagatcatcc
     9961 cggcaaactc tacctggtca ccgacaaccg gtgccgcgcc taacaactgc tggacaattt
    10021 cacccagcgt ttgctgctta tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg
    10081 caacatcagc atatttggcg ctggcttcga gaatgaagtc accaaagaag cgttgatcca
    10141 gcgcgaccgg cggcgactgg ctgaacagtt taccgagcgc cggaagatcg cgttcccgac
    10201 caattacaca caacacatcg ccttcgcgca gtcgggtgct gccggtagga tgaagcaact
    10261 ggttatcacg aaacagtgcc gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg
    10321 ccgcgcccac gcaccattta tcggcactca attgataaac aaactgctcc cacggatttt
    10381 ccggatgaat atccaggcca acgcgtgaca ccggacgtcc cactggcgga accaccactt
    10441 tggctttttt agccgcccac gagagtgatg ttccctgcaa gagcagtgaa accagaacca
    10501 caaagaaggc gacattaaag aacagacgtg cattctccag ccccgccatc atcgggaaca
    10561 cagccaggat gattggcacc gcgccgcgta atcctaccca gctgataaac acgcgctcac
    10621 gcagattgaa accgcggaag gggagcaatc cagcaaatac cgaaagggga cgggcgaaga
    10681 atatcatcca tgccgacaaa atgagcgccg gaatggcaat aggcagcaga tcgcttgggt
    10741 taaccaacag ccccagcacc aggaacatgg cgatttgcgc cagccaggcg aggccgtcga
    10801 aattttgcag gatgccgtag cggttgcgaa tcggccgatt acccagcaga aaaccgcaca
    10861 gataaacagc cagaataccg ctgccttcca gcgccgtggt taaagcaaaa attaaaatac
    10921 cgccgcttaa tgccagcaat ggatataatc cggcgggcag ggcgatgcga ttaatcattt
    10981 gcagcagtaa ataaccacca ccaagtccga tgacaatccc gaggccaaat tgctgcagaa
    11041 tatcgaccac gaacatccaa ctgacactgc tttcatgttg ctggatcatc gcaatcaggg
    11101 taatcgtcag aaagaccgcc attggatcat tactgccgga ttcaatttcc agcgtcgagc
    11161 caacacgttc gttaagcccc ttaccaccca gcagagaaaa gaccgctgca gcatcggtag
    11221 agccgacgat agcgccgatt aataagcctt caatcaaatc aagattaaac agccacgccg
    11281 ccatcatgcc ggttaaacca gaggtgataa gcacgcccag cgtcgccagc gacagtgccg
    11341 gtcctaacgc cacacgaaag gaactggcct gagtgcgcat cccgccgtcg agcaaaataa
    11401 tcgccagtgc caggttactt accatgtagg cgaaggggta attatcaaac gggataccgc
    11461 cgacgccatc gactcctgcc agcatgccga tcgccagaaa aataaccaga ataggaatgc
    11521 caagacggga agaaaatgaa ctaagtaata tactgctggt gacaaggatg gatcctaaga
    11581 tgaaaaggct aattattgtt gtggcatcca acggtcggtt actcctgatt acgctgtctc
    11641 ttatataaac cctaccatat tagcggcaga gacagcgttt tacttagccc tgaagcgtga
    11701 tttttttaca ttttaagaac aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg
    11761 ttattcagaa tggcatgtgc gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa
    11821 tcgaggccgg taatgagtgg aatcgacagg cgggaacgca aaaacgcgta cactgactcc
    11881 aggttgtaac ctgcgtcata atcattgggc gtgctgccgc taaagctacc gagaataatc
    11941 gccttctgac gtggcaaaat tcccgcatga tagagctgca acagcatacg ttcgacgcgg
    12001 aaagggtgct cgttaatatc ttccagcacc agaataccgt tctcaatttt tggcatccac
    12061 ggtgtaccaa tcagtgaaat cagcatcgca agattgcctc cccacaacgt gccttcggtc
    12121 tgacatgtcg ggccttcccc ttgccattca atggtgaagg tttcattgcg taacgccagc
    12181 cagaaatggt gctcggtaaa ggcgttcagt tcatccgcgc caaagtttgc taccagcatg
    12241 gggccgctaa aggtgatgac attgccttgc gccagaagac cgcactgaat ggcggtaaaa
    12301 tcgctatgcc cgcatatgag caacgggtca tattgttggc gagcaaccag tgcctgccag
    12361 tcaatatctg ccagtaaacg actggcaccg taaccgccgc gtacagccag cacgatggtg
    12421 ttgggggtgg tcagtctggc aagggaattg agatcttcca ggcgctccgt ttccgtaccc
    12481 gcaaaacgct cacaacgacg ggcaataacc tcgacgttat tgacctgatg cccctcgtcg
    12541 gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa tgcagtaacc cgatggggca
    12601 attaagtgaa acagagacat ggcaattcct tgctgacaac agaaacgaaa tgtatatcat
    12661 gccgcttagg tgtgccgttg tcacctcaac ggcgattcca ggctataagg atagaagaag
    12721 tgaaattgag atggtttgcc tttttgattg tgttattagc gggttgttca tcaaagcatg
    12781 actatacgaa cccgccgtgg aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc
    12841 caataagcca gaaagccggt gcagcctggg gcgtcgatcc acaattgatc acggcgatta
    12901 tcgctatcga atcgggtggt aatccgaatg cggtgagtaa atcgaatgcc attggtttga
    12961 tgcagttaaa agcttcaacc tccggacgtg atgtttatcg ccgtatgggc tggagtggtg
    13021 agccgacgac cagcgagctg aaaaatccag agcgtaatat ttcaatgggg gcggcttacc
    13081 tgaatattct ggaaaccggc ccgctggcag gcattgaaga tccgaaggta ctgcaatatg
    13141 cgctggtggt gtcatacgct aacggggcag gtgcgctgct acggactttc tcgtcagatc
    13201 ggaaaaaggc gatcagcaaa atcaacgatt tggatgctga cgagttcctc gatcacgtag
    13261 cgcgaaatca tcctgcgccg caggctccgc gctatatcta caaacttgag caggcactgg
    13321 acgcgatgta aatcagtcgc gcactttgtc cgctttttcc cgggcttctc gctcgagaga
    13381 gaaaataatc cgctgtaatt gccgctccac cgtcgggctg acgttaagaa aacggaagct
    13441 cagacgggga gtggtgatgg tttcattctt gccatcaatc actttgcgct cgctgatgga
    13501 gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc atgttgactt caatctgagc
    13561 gaagcgcatg ccttcttgta attcggcagg ctttgctgtt tccagtaatg cgcccatgcc
    13621 gcctaacgac aaatcataca ggcggaaacg taacgtactg ttatccgcca gtttggtctg
    13681 gcaaaaataa ggcggatgga gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa
    13741 ccataaggtg ggaggcggta cggtgataaa tgccggaagc tgcaagtatt cactctgctg
    13801 tagttgttca acagtaaact cgactttcgc accctgagtt tcggcggtaa tggtaatgtg
    13861 ctgtgccttt agcacggcga tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt
    13921 atccggggtt attgccagta ttttgctgat cagctgcccg ccattccaac tgagacgcaa
    13981 aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg acggctaacg gattttgttt
    14041 caggaactgc tcatggtaat gactcacgcg gaaaaactcc tgactgacaa actgtctttg
    14101 gtttatcggt cacagttaac aaaacttaat acaaatgcgt gaatattttt tacatgttgt
    14161 tcttaaatca gccgacatac gcccagcatc gatcccgccc ttgcctatac ttagagcgtt
    14221 gacgtaagca tttcttgcgt ccgattcatc gaaacgaggg cctgaacatg ggaattattg
    14281 cctggattat ttttggcctg atagccggca ttatcgccaa gctaatcatg ccggggcgtg
    14341 atggtggtgg atttttcctg acctgtattc tcgggatagt cggtgcggtg gtcggcggct
    14401 ggctggcgac catgtttggc attggcggct ccatcagtgg ttttaatctg cacagcttcc
    14461 tggtggcggt ggtgggagct attctcgttc tgggcgtatt ccgcctcctg cgaagagaat
    14521 aagattttca taaggcggat agcgatacag atgccgctat ccgctttcac atcagaacgt
    14581 atactcgaca cctgctttac gggtggaaca aatcaatata gcacttagat gcccgataaa
    14641 gtcggttccc ggtaacaaca acagaatcct tttttaatta ttgtttcgtt gtttttggtg
    14701 tgattcagaa aataaatatt ttagacagta gccagtcagg cgttaaaaat ggggcggata
    14761 gtgaggcggt tttggctgcg cttattgaac atttagaatt gatcaatcct tctggcagat
    14821 taagccaaaa tacccgttcg gcaatgcttc agttacgcga agaggaatgg tctgaattct
    14881 ttttttggtt gctaaattct ctggagtgtc tggattatgt gattattaat ttagctccag
    14941 aaagcaaaaa aacgttgatg agtgaacacc gtaacaatat tcaggtagca atcgatgctt
    15001 tatataggca acgtcggtgt aaatcaccgg gtgatgaaag tgaaacactt acccgtagaa
    15061 atgacgctat cttcggcaac cacgtatggc aaacatttgc gcaatacttc ccgccaggac
    15121 ttgaaaaacc gtctgtgtag aaaacaccat agctatggtg ttatctgccg acaacgccat
    15181 ccggagaagc cgaatgacgt tgtcggagta agcgctggtt aaggtgtggg ttgtgcctct
    15241 ttggttgagg gttgcgtcgt tgctgactta acggtcggac gcgtcgccgg aacattgtca
    15301 cacggttgct ctttcgggca gatcaaatcc agcattttca gcgtcacgcc attggtccag
    15361 ccaaagccat cctgtaatgg atattcgcca ccgccgcccc ccgttccggt ggtgctgaca
    15421 tcatattttt ccaccagctt tttctcccgg tcataggtgt gctgaacatt ggtcaggaag
    15481 tgccagctaa tgtccatcgc cacctctttt tgcccgtagt tttgtaatcc ttctgtcgcg
    15541 acccactgta acggtgccca gccatttggc gcatcccatt gttgcccact tttcaccgac
    15601 gtggtgttca ggccgccggg ttgcagcaga tgtgttttcg tcgccgtcgc cattttgttg
    15661 gcgcgatctt tcgctgccgc attgacgtac agcgggaaca gggcggccgc ggttaactga
    15721 ttgcgcactt tatgactttt caggtcgtaa tcggcatacc agccttgttg atcgttccac
    15781 atgtattttt cgatcccttt ttgacgggca tttgccagcg tttcgtactg gtttgccatc
    15841 gcgttatctc cggcagcttt gctggcgcgg gcgaggattt tttccatttt aaacatcagg
    15901 ctgttcagat cgaccggtac gatgctggtg gtgcgtaagg tatttagttg atgcgggttg
    15961 tccatccagc gcgagctgaa atcccagcca gacgcagcgg cagagcgcag gtcgcggtaa
    16021 atttcagtgg caggtcgatt cggattgctt ttggcggtgg caatatcttc cacccatgac
    16081 tctggtcgtg gcgtatcgcg atcgtcccag tagcggttga gaagggtacc atcctgaagt
    16141 tttacaacgc gtttttcctg ttgtccggct tgcaggtttt caacaccgtc catccagtaa
    16201 gcatattctt tttgcatttg cggcaggtat tgcttcaacg cggcatcgcc ttcatgctgc
    16261 gccagtaact ctaccatcag ggcaaagaag ggcggttgcg agcggcttaa atagtaactg
    16321 cggttgccgt tgggaatatg accgtaagtg tctatttcat gagcaaaatt ggccaccata
    16381 tccgcgactt tatcccagtg accgctttcg gcaagtccta acatggtgaa gtaactgtcc
    16441 cagtaatata cctcgcgaaa acgtccgccc ggcacgacat aaggttccgg cagcggtaac
    16501 agagaatccc atttttcggt gttttcggta gaacgcgtta ataccggcca aagtccgtca
    16561 atatgttcgc gcagtgactg cccctctggc ggaacatatt tctcgccctc tttcggcagg
    16621 gtgaaattga cgttaacgaa atggcgcaga tcaaatccgc tctggttttg ctgcatccga
    16681 taatcagcaa ggatcatcag cggatcgctg ttcggcacgg catcggcaaa ggttttttgg
    16741 tccggaaaaa gtttggcgtt ttgcacatca ttaaacagcg gccctaataa aatatcaggc
    16801 ggctgtggtg ttaccggtgt ttcttctgcc tgcaccgata gcgcagcgaa acacaaaaag
    16861 atacaggctg gaattaacgc cattttttgc gggcgagaag gtgcggggga tttcatcaat
    16921 cattctcctt tggcgaaacc gaataaaacg gtattcaagt atcagaaaac cttagttcag
    16981 gatcgcacta gctgcatgat cgaaatctca ttctgcgaac tgtcaggcat tttagaaaag
    17041 ggaattaaag ggataaataa cggtgaaata gagtgatcaa ataacgtctg tttattagcc
    17101 agtcagcgac gttgggttcg cgattagcga agaaaaatca tcataaattg gtaaaaaata
    17161 taacaggtag ttaaaaaaca tcagtgctgg gtaaattgcc ggatgacatc agaacgatgc
    17221 catccgaacg gtggcttaac cctgacggtt gaaacgttgc gttttaacgt ccagcgttag
    17281 cgtttcttct ggttgtatcg catacagttt ctcaccctgc tggcaaatcc agccaatccc
    17341 cagttcacgg gcgattaggg cgctgtggga taccggactt ccggcgctaa ggcagatacc
    17401 ttttacaacc gccggatcca gttgcagtac tgtggaagga taaatgttct ccgccagtag
    17461 aatagttggc gagttaaact gcgggagttc ttctttcgtt tgggtcaggt ggaccagggt
    17521 gcgatgcaga agatcgtcca catcaatata gcgagcttgt agatattcat catccagttg
    17581 ctggtattgc tggctaagtt ctttaagaac ttgctgccag gcatattctg ccgtgcaatg
    17641 ttcatgctga aggagttcgc ttgccgccgc cagcagttcc ggatcatcta acagtgtatg
    17701 gtgaccagaa aagattgcgg caatatcgtc aagcccgctg gcttctgctt tcgctgttaa
    17761 cgtcatcaga tctaataacg tgaagtcaat agcctggcgt aatcgatctt gttcttcttc
    17821 cacggtcagg gttgattttg cctgtaccgt acataaaact ggttgataat aaaaggcttt
    17881 acccgaaaca ggcggaacgg gacgcagagt aggtggagcg acttcctccg tttcaccaaa
    17941 gttatcttca gccagctgac ggaaagcgat cagtgcctct tcagcttctg gccctttcgc
    18001 aatcaggcgc agcgtatcgt tatagcgaac ttgtagtaac gcaatctggt taatactctc
    18061 tggtgtgacg catttgccgt ttttttccag caacatatcg gcattaaatg tcgataaggt
    18121 ataaaccagc cgggaggccg gacgtacatg caggccgtta cggtttttta tgaccaccgc
    18181 cagagaacgg gcttcttcat cgtacgcagg acatgtgtca gagatttcag tgtcggagga
    18241 cggtaaaccc agttgttcac gtttggcttc cagcgcatgc atagcgtcaa agataacttt
    18301 gtcgatatcc gctcccgagg ccgcgctgac cgttgctgcc agtgtacctt cgaccaacgg
    18361 cgcagcacac aaacgtactt ttgcggcgat cttgggagcc agcaattcca gcgcagtttc
    18421 agcactcaat aatgcgctac ccatatccat catgaccagc acatggtcgg catcagcaac
    18481 agattcgatg gcctccatca ctttgacggc atcggtacca ataggatttt gtggatcgtc
    18541 aattcccgcg gcaatggcga ttttacaact atcactcatt aacatctgac gggctaattc
    18601 accgacacct tctcccagtc ggctgctatg tgaaactatg accaggttta ccatcaccaa
    18661 ttccttactc ttttgcggct aacgccaaca tttgcatcat aaacatcacc gaggtcgcgc
    18721 cgggatcctg gtgaccaata ctgcgttcac cgagataact ggcgcggcct ttgcgggctt
    18781 gcatcgtaat cgtactttgt gcagcggatt cggcgattct actggcagct tcgagcgcca
    18841 ccggaacaga gagattttgc tcgctggact gacgtaacga ttccaccacc ggcacccaca
    18901 catcacacat ggttttatcg ccaggttcgg ctttcccgcg actgattacg ccgtccgcgc
    18961 catcgcggaa catctgataa agctcttcca gtgtcaggct ttgccgtgcc tgggtcgcct
    19021 gtgcggcgcg gataaagaag gtaccgaaca gcggaccact ggcaccaccg acgctggaaa
    19081 gcagcgtcat accggtattc ttgagaatga aaccgatatc tttatctgcg atagcaggga
    19141 gtttttccac cactttgcta aagcctcggt tcatatttag cccgtggtca gcgtcgccaa
    19201 tttcgcgatc cagtccggta agatactcgc tctcggtgct gaaaatatcg ccacaacgag
    19261 tgagccagtt aacaatttga gttctgctca gtgacattgc tttctcctta tttaccccag
    19321 ttaagggccg gggtgtggac cggggcgtcc cagagtgcca gcgtttcgtc atcaactttc
    19381 agtaaggtga ttgagaaacc ggtcatatcc agtgaggtgc agtacgcgcc aattaaatta
    19441 cgttcgatag tcaatcccgc ttgctggcaa cgtgtggtca ggcggttata gacgccgtac
    19501 agctcagaaa gcggagttgc gccaagattg ttaaccagcg caatcacccg atcgccagac
    19561 tggagcggtt gtttggtttg ttgttcttcc tgccaactgc cttgttgata atcccagaaa
    19621 cgcaaagtgc gatggtatga gccattttcc agcagggtgt cgaacatttc atcgacggtt
    19681 tgatcaaggg aagagaagga gcggcggtca atacccggct caccatgaat gccgacgcca
    19741 aactccatct cattatccgc cagggtaaaa gaaggtttgc ccgcggcagg aacggtacag
    19801 gcaccgagag cgatacctat tgagtggcct tgattattca acttacgccc cagttccgca
    19861 caggcgtcca gtgagtcgcc acgctccgcc gctgcgccta cgagtttttc aattaatacg
    19921 gtgttggcaa cgccgcgtcg cccggcggta taaagactgt cttttaccgc aacgtcgtca
    19981 tcaatgacca cagtggtcac ttttacgccg ctatcgtgca gtaactcggt cgctgtttca
    20041 aagttaagaa tatcgccggt gtaatttttg ataatcaaca gtacaccttc gccgccatca
    20101 atttgcatgg agcattcaaa gattttatcg ggcgtcggtg aggtgaaaat ttcgcccgga
    20161 caggcccccg aaagcatccc ctgaccgata taaccacagt gcatcggctc gtgtccgctg
    20221 ccgccacccg acagcagggc gacttttcct gcaacagggg catcagctcg ggtgacatac
    20281 accggatcct gatgcagtgt cagcgatgga tgcgctttcg ccagtcctgc cagttgttcg
    20341 tccagtacgt cttgcacatc attgatcaat tttttcatta ttttgctcca gcaattacgg
    20401 tagggcatgg atgatgttca acgacacggc gaccactgac tgccgatgaa tccattgtgc
    20461 atcaggcaag ggaaaagaaa attagcccaa attatgtttc atagtgaaac atatgcttta
    20521 atgaatgttc catattgaaa cttttacgtg tattaatact taaaattgcg agccggaaca
    20581 ccttttgtca taagggatgc gggatatgag tggcgctttt aacaacgatg gtcggggcat
    20641 atctccctta attgcaacct cctgggagcg atgcaataag ctgatgaaac gggagacatg
    20701 gaacgtacca catcaggccc agggcgtgac atttgcttct atttatcggc gtaagaaagc
    20761 gatgctgacg ctcgggcagg ctgcgctgga agatgcctgg gaatatatgg caccgcgaga
    20821 gtgtgcgctg tttatcctcg atgaaaccgc ctgcattctc agccgtaatg gcgatccgca
    20881 aaccttgcag cagctaagtg cactgggatt caatgacggc acgtattgcg ccgagggaat
    20941 tattggtact tgtgcgctat cgttagcggc tatctctggt caggccgtga aaacgatggc
    21001 cgatcaacat ttcaaacagg cactctggaa ctgggccttt tgtgcaacgc cgttgtttga
    21061 cagcaagggc cgattgacgg gaacaatagc gctggcgtgt ccggttgagc aaactaccgc
    21121 agctgatttg ccgttgacgt tggcaatcgc ccgcgaggtc ggaaatttac tgctgacgga
    21181 cagtttgctc gctgaaacta accgtcattt aaatcaactt aatgccctgt tagaaagtat
    21241 ggatgatggc gtgattagct gggacgagca gggtaatttg caatttatta atgcccaggc
    21301 ggcgcgggtc ttgcgccttg acgcgacggc aagtcaggga agggcaatca ctgaactctt
    21361 aacgttaccc gccgtattgc aacaagcaat aaaacaggca catccgctca aacacgtaga
    21421 agcaaccttt gaaagtcagc atcagtttat tgatgcggtg ataaccctta aaccgataat
    21481 agaaacgcag ggaaccagct ttattttgtt gctccatcct gtggaacaga tgcggcagtt
    21541 gatgaccagt caattaggaa aagtcagcca taccttcgct catatgccac aggacgatcc
    21601 gcaaacccgc cgcttgattc attttggtcg ccaggcggcg cgcagtagct ttcctgtcct
    21661 gctttgtgga gaagagggcg tgggaaaggc actgctaagt caggcaattc ataatgaaag
    21721 cgagcgtgct gcaggtcctt atatcgccgt caattgtgag ttatatggtg atgcggcgct
    21781 ggcggaagaa tttattggtg gcgatcgcac ggacaatgaa aatggccgtc tgagtcggct
    21841 ggaactggca cacggcggca cgctgtttct tgaaaagatt gaatatctgg cggtggagtt
    21901 acagtctgct ttgcttcagg ttatcaagca gggtgttatc acgcgactgg atgcgcggcg
    21961 tttaatacca gttgatgtca aagtgattgc cacaacgacc gcggacctcg caatgctggt
    22021 ggaacaaaat cgttttagtc gccagctgta ttacgcgttg catgcatttg aaattaccat
    22081 cccgcctctg cgtatgcggc gtggcagcat tccggcgctg gtgaataaca aattacgcag
    22141 tcttgaaaaa cgcttctcta cgcggctgaa aattgatgac gatgccctcg ctcgcctggt
    22201 ttcctgtgca tggcctggca acgattttga actttacagc gtcatcgaga atcttgctct
    22261 gagtagtgat aatgggcgca ttcgcgtcag tgatttgccg gaacatctgt ttaccgagca
    22321 ggcaacagat gatgtcagcg ccacacgcct ttccaccagt ctgtcatttg cggaagttga
    22381 aaaagaggca attattaacg cagcccaggt cacaggcggt cgcattcagg aaatgtcggc
    22441 tttacttggg atcggccgca ctacgctgtg gcggaaaatg aagcaacatg gcattgatgc
    22501 agggcagttt aagcgccggg tatgaaagac agagacgatt tctgatacat cagagtgatc
    22561 tatatttcat tccggcgcac gctaacattt ttcagcatcg tttaagggct ggtctatccc
    22621 gcacttaaaa gctgaagcga tatcctccgt tgacttgctt ctgatcaaag aggttactct
    22681 gcgtgtaatc cgcttcgagg tagaatgtgt gctgtttgtt atactgtgca ctgactccca
    22741 cgccgttatt ccagccatta cctttgaaac tgtacttctc ccgggagttg ttcaacagat
    22801 agtctgtatc ccctgaaaac tcgcggatag ctccagtctt cacatacata ttcagctggc
    22861 tgttgcctgc ggtgatgtca tatcccagta tcatgctggc acgccccagc agcgattcgt
    22921 agtgattcag atgtatattg agaccattac tcgccttcat agccatctca ttctggtggc
    22981 tgtatgtgag ctgggtttgc ggctctatat agaacccata accagtaggg gtcaggttga
    23041 acctctgccc ggcttccagg gagatgctca atccattcgc agtgccgttg gcgttaacgc
    23101 cgttgttctg actgtccagt acgtgaaaac tatttttctg gcgcgatgct tttacaacga
    23161 gatcgctgta aaaaccgttg tgtgccatgt aactggcata cattcccatg tagtctgaac
    23221 gtgcggtacc gtcgcctccg ctatagtccg gcgatgcatg tgttgagcca atatacagac
    23281 cgacatacaa cggcattaca tcagagagac gtttatcccc accaaactgg ataccgctgt
    23341 aacccatgtc aaagccgctc agtttgccac tggcgaagga gtccaggctt cccccataac
    23401 tgcgcagcca gatattaccg tctttactct gatttcgcag gtcacccatt cgttgcatca
    23461 gcgtacggtt ttcaacatag tttaataagt aaccgacatt gagataattg ccgccagcat
    23521 ctgcggtcgt ggtgggttta ggtgtgggag tggggttagg cgtaggatcg ggttcaggcg
    23581 taggatcggg gttgactatg ggaggctgag cgggagccgg tgttggttca ggattaggag
    23641 tcggttccgg aactgtcccg gaagcgtaaa gctcccagtt agtgccattt ttacgcacat
    23701 cgtacagata tccccccaac tcaacctgag aagacgcgct gaacgaggcc gcaccgtcag
    23761 tggtttttac cactgtcaga acttcatttc ccgtagtggc ctcgctgccc tggttgcgga
    23821 tagccaatac gtgattacca gcactacttc cgctgatatt caataaatcc cctttattat
    23881 taacgccatt accctcgcca acaacatcag cacgcataat aaaggtactg ttaccgctca
    23941 ggttctctac gtttaatgtg gcaaatgtgc cggcagttga cccgtggctg gcaaaatcga
    24001 cagttgaatg gctcagcgcc agcgtgtcga gattagaatt acttgttacg ttccagacgc
    24061 tgttattcat tgcaacgtcc agtttcccgc cattgacatt atcgctgagg gaggaacctg
    24121 tccaaaccga ccctgggtgc atatctagat tgattagccc acctttggaa agaacgctac
    24181 cgttgataag catacgaccc gaggcgttga tgcggctggc ggcataacca tcgtcatgtt
    24241 gcgttgcaat ggccatctgg tcgggtgtac tcatatcaat gaccagatcg ctcgtgaggt
    24301 cgatctggct gttggtcatg gcataaattc ccctggctcc tgccgcgccg gtgatagcca
    24361 aactgtctcc ggttattcta ccgccgctga gcgcccacaa acccagcgcc agactgccat
    24421 tacgatcaac tgtaatatcg gtattttgca tgttgacaac agccgttgcc gtctgggccg
    24481 aggcaccata agaaccgccg gaaaagatgc tgtttcgttg cgctgccgtg ccagaaaaat
    24541 tgattgtcgc gtctgaacca ctggtgacga ggccaccgcc ctgcgcggaa gaaatatggc
    24601 tatctgcacc gatagtggtt gtaccaccac gaacttcgac gccattggcc gcggctccag
    24661 ttacatcaac agtgagtgca ttcgcgctaa cctggccaaa gctccagagg ccgtgtgcat
    24721 tatcgccatt ggttttaatg gaactgtttg ttccgagatc gacaacagag tttttctgta
    24781 cgtttatccc catggcgctg tagccctgaa catcgattgt caggtctgta gccgtaaaac
    24841 gcgcagcacc attggcgtta ttgccgttga gaccaccgat ataaacacct gtacttccat
    24901 cggtcgtgat tttacttccg cttccaagat cgacactggt gccatagtca ttgatggtta
    24961 ggcctatacc gttcgagttt tcaatggtga attgagtggc tgttagtgtt gagctatgcc
    25021 caataatgat gccgtcatcg ttactcttaa tggtgctgcc tgtgcctaag tcagcatggg
    25081 tatagtcacc aattaagtta atgccgatgg cagaggtttg accaacaaca tctactgtga
    25141 gtcggttagc tgtcagggag ctgtttttgc cctgaataat gattcctttt gcatatccag
    25201 aggcgtcgtt gacattgacg gtgacatcat tacccaggtt gatattgcca ggcgtgtttc
    25261 ctgctggggt cattacacca tagaggtttc ctgatggatc ggcggtatct cctgtaattc
    25321 tgtcaccttc attaatagta atattggtgc catattgact tcctgtatca gcagctatca
    25381 gcagggggga aaagataatt gcattcaaac caatagcgcc aaattttatc agttgaattg
    25441 agggcgaacg gatttttaat tcagcgagac gatctgcttt ggtattccca ttgtgttgtt
    25501 tgatgcccat acc
//
LOCUS       DAZFRJ010000055        25462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000055 DAZFRJ010000000
VERSION     DAZFRJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.055"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            198..545
                     /gene="grxD"
                     /locus_tag="ACHKUN_002037"
     CDS             198..545
                     /gene="grxD"
                     /locus_tag="ACHKUN_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO6660291.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(596..5212)
                     /locus_tag="ACHKUN_002038"
     CDS             complement(596..5212)
                     /locus_tag="ACHKUN_002038"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO6660292.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(5305..5952)
                     /gene="rnt"
                     /locus_tag="ACHKUN_002039"
     CDS             complement(5305..5952)
                     /gene="rnt"
                     /locus_tag="ACHKUN_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO6660293.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(6055..6462)
                     /gene="gloA"
                     /locus_tag="ACHKUN_002040"
     CDS             complement(6055..6462)
                     /gene="gloA"
                     /locus_tag="ACHKUN_002040"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO6660294.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6543..7640)
                     /gene="nemA"
                     /locus_tag="ACHKUN_002041"
     CDS             complement(6543..7640)
                     /gene="nemA"
                     /locus_tag="ACHKUN_002041"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO6660295.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7677..8276)
                     /gene="nemR"
                     /locus_tag="ACHKUN_002042"
     CDS             complement(7677..8276)
                     /gene="nemR"
                     /locus_tag="ACHKUN_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO6660296.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8379..8618
                     /locus_tag="ACHKUN_002043"
     CDS             8379..8618
                     /locus_tag="ACHKUN_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO6660297.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8667..9563
                     /locus_tag="ACHKUN_002044"
     CDS             8667..9563
                     /locus_tag="ACHKUN_002044"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO6660298.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9644..10165
                     /gene="sodC"
                     /locus_tag="ACHKUN_002045"
     CDS             9644..10165
                     /gene="sodC"
                     /locus_tag="ACHKUN_002045"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6660299.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10166..12178)
                     /locus_tag="ACHKUN_002046"
                     /pseudo
     CDS             complement(10166..12178)
                     /locus_tag="ACHKUN_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(12178..13035)
                     /locus_tag="ACHKUN_002047"
     CDS             complement(12178..13035)
                     /locus_tag="ACHKUN_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6660300.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(13038..13274)
                     /locus_tag="ACHKUN_002048"
     CDS             complement(13038..13274)
                     /locus_tag="ACHKUN_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO6660301.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13475..13909
                     /gene="slyA"
                     /locus_tag="ACHKUN_002049"
     CDS             13475..13909
                     /gene="slyA"
                     /locus_tag="ACHKUN_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO6660302.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13956..14423)
                     /gene="slyB"
                     /locus_tag="ACHKUN_002050"
     CDS             complement(13956..14423)
                     /gene="slyB"
                     /locus_tag="ACHKUN_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO6660303.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14697..15806
                     /gene="anmK"
                     /locus_tag="ACHKUN_002051"
     CDS             14697..15806
                     /gene="anmK"
                     /locus_tag="ACHKUN_002051"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO6660304.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15904..16233
                     /gene="mliC"
                     /locus_tag="ACHKUN_002052"
     CDS             15904..16233
                     /gene="mliC"
                     /locus_tag="ACHKUN_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO6660305.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            16292..16948
                     /gene="pdxH"
                     /locus_tag="ACHKUN_002053"
     CDS             16292..16948
                     /gene="pdxH"
                     /locus_tag="ACHKUN_002053"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO6660306.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            17077..18351
                     /gene="tyrS"
                     /locus_tag="ACHKUN_002054"
     CDS             17077..18351
                     /gene="tyrS"
                     /locus_tag="ACHKUN_002054"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO6660307.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18410..19273
                     /gene="pdxY"
                     /locus_tag="ACHKUN_002055"
     CDS             18410..19273
                     /gene="pdxY"
                     /locus_tag="ACHKUN_002055"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO6660308.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19317..19922)
                     /gene="gstA"
                     /locus_tag="ACHKUN_002056"
     CDS             complement(19317..19922)
                     /gene="gstA"
                     /locus_tag="ACHKUN_002056"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO6660309.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(20028..21530)
                     /gene="dtpA"
                     /locus_tag="ACHKUN_002057"
     CDS             complement(20028..21530)
                     /gene="dtpA"
                     /locus_tag="ACHKUN_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO6660310.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(22141..22776)
                     /gene="nth"
                     /locus_tag="ACHKUN_002058"
     CDS             complement(22141..22776)
                     /gene="nth"
                     /locus_tag="ACHKUN_002058"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO6660311.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22776..23471)
                     /gene="rsxE"
                     /locus_tag="ACHKUN_002059"
     CDS             complement(22776..23471)
                     /gene="rsxE"
                     /locus_tag="ACHKUN_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO6660312.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23475..24095)
                     /gene="rsxG"
                     /locus_tag="ACHKUN_002060"
     CDS             complement(23475..24095)
                     /gene="rsxG"
                     /locus_tag="ACHKUN_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO6660313.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(24099..25157)
                     /gene="rsxD"
                     /locus_tag="ACHKUN_002061"
     CDS             complement(24099..25157)
                     /gene="rsxD"
                     /locus_tag="ACHKUN_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO6660314.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(25158..>25462)
                     /locus_tag="ACHKUN_002062"
     CDS             complement(25158..>25462)
                     /locus_tag="ACHKUN_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660315.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         6321 a         6575 c         6643 g         5923 t
ORIGIN      
        1 tgcagcagtg aaagctaaaa atggttataa atacgccagt ttgccgagca tctgggaaga
       61 aacgaatatt taaaatgcaa caaatagagc aaagggataa aatagcaaaa gcgtgatgaa
      121 aatgttattt tccgatgccg cactgacccg ctacaatgtc agattactgc cgtaaagaag
      181 tttgaggaag caagacaatg agcaccacta tcgaaaaaat ccaacgccag attgctgaaa
      241 acccgatcct gctgtacatg aaaggttcac cgaaactgcc gagctgcggt ttctctgccc
      301 aggcagtcca ggcgcttgcc gcatgtggcg aacgttttgc ctatgttgat attctgcaga
      361 acccggacat tcgtgcggaa ctgcctaaat atgctaactg gccgaccttc ccgcaactgt
      421 gggttgacgg cgagctggta ggcggttgtg atatcgtgat cgaaatgtat cagcgtggcg
      481 aactgcagca gctgatcaaa gaaactgccg ctaaatacaa gtctgaagag ccggacgcgg
      541 aataatccgt cctgcaatac tgacggatgc gaacgggcat ccgtcagtcc tttctctatc
      601 cccaatccag cccttgtggc gaacttgaaa atcccgcctc acgcagcagc gtaaacatcg
      661 gcgtttgccg gaccggtaga tcattcactt ctgttagcgt aaagcgcagc cgtggttcgc
      721 gacgcagtgc ggtagtcagc gcgtggaaaa cctctggggc gagtaattcc tctttttcct
      781 gccagaccag catttttttg ccaccttgcg ccagatagag taacaatttg ccgccagaaa
      841 cgaccaccag cgcacccgcc cgacgcgttg gaaccagcgt tgccggatgt gcaggccagg
      901 gcagaagatt tccccacaca ttagccggat cgttggcaga gagcgccact ggtgtatagt
      961 ggcgcgtttg cgtggcttgt gtcgccagat cgcgcaatcg gtcaatagtc agacgttcag
     1021 cgaattgcgc gccacccaga ccttctacaa aacgacctcg cataattcgc ccggagtctt
     1081 ccatacttcg acaaagcgtt tgcatcgatg gaaacccgcc agggatattt tcggctatca
     1141 ccgcctgacg actgatgatg ccgtagcggt cgagcatatt ttccgccagc gccagcatcc
     1201 tttcggtatc gtttagtggc tccacctgca ataacgacca gcgtccagcc agatttggtg
     1261 tgttgtaaga taccagcggc gagacgggtt gcgcatagac aggacgtcca cgacgagccc
     1321 ggtgactgcg gcgagttgag gtgcgtgcgt tggaactgct gcgggtgagg gcgcgtaacg
     1381 gtgcccaaat gtcgctggtg atgacgcctt gccagaccag cgcccataac gcctcttgca
     1441 gggcagagag atcaaccgat tcgccgattt tgtcgcgtat ccgttggctg atttgttgtg
     1501 caaaccaggc tcctccgtca gccagaacag cgattatcgc ttgctgcagc gccgaacgat
     1561 tcgcctgatc cgcttcggcg ggagtgaacg attctgcggc atattcctgt agatgcagtg
     1621 ccaccaggcc gtcatcttca cccagctttt tttgtcccga ccagataacc gctccggttg
     1681 ccagtaattc atcgagcatt tctggcgaat agtcgcgtac gcgggcaggc aggatctggc
     1741 tttcccagag tgaggcgggt aaaccgactc cggcaagctg ttcgatcacc cgcatcacgc
     1801 catctacgcc ctcataaacg cctggcgatg ttgaggcaaa gagcgccggg ctaccatcgg
     1861 tggcgggtaa tacgccctga cgttccagca gcaatcgcgc ataggtcgtg gctgcaacgg
     1921 gacgcgtcgc ttctctggcg gcttgcagcg agcgcaaacg cagacgacga aatacttcat
     1981 cgctcaccca gatgtcttgt tgcagattca tcaccagacc ctgttcacgc agttgctgaa
     2041 gctgctcttc gacaatggca ataccgagac taaactcatg agccagttgt tcagccgtga
     2101 ccagagcatg agcccggaga tagcgcagaa agaggtcgcg aagcgggtaa ctgactctat
     2161 gtaaataaat ctctggcaat gactctggta gtcgtacgcc gagggcatca cgcagcctgg
     2221 cggcatcatc catacaggca agacgctcct gtccgctaat catcgctggg aagattcgtt
     2281 ttactgcaag aagattttcc agatacgacg caacctcttc actgctgcct gtatgccgtt
     2341 gcgccaggtc ttcaacggtc attggcccca gttcgcgcag caggtcgaac aatccttctt
     2401 cacctttcgc tcttctgcca ggagccagtc gttgcaactc ttcttccacc tggcgaatga
     2461 cctgcgggtc gagtaattcc cccggatcga cctgtccgag tagattgcgc agtaactcgc
     2521 tgtccagcga cagtacggat gcccggcgct ctgccagcgg ggcgtcgctc tggtacataa
     2581 attccgcgac atagccgaac aataaacttg tggcgaaagg cgagggcgta gtggtcgtta
     2641 catcggatat ttgaatttcg ccaccgttca ggcgacgcat caaacgttcc agtgcgggaa
     2701 gatcataaac atcttgcaga cattcgcgta gggtttcgag aatgaccgga aaatccggat
     2761 atccctgagc gatttccagc aactgactgg cgcgcagccg ttgttgccaa agcggggtgc
     2821 gatggcccgg agtgcgcccc ggcattaata atgcccgcgc ggtgcattcg cgaaaacggg
     2881 cggcgaaaag tgccgagctg cctaccgcct cgcggacaat ttgcagcaac ttttctggtt
     2941 caaacaaaaa aatcgcggcg tcgggcaatt taccatcagt gtcaggaatg cgcgcaacaa
     3001 tgccgtcatc actggcgacc acagacgcgt cagcgcccca tagggcatgt attcgcccgg
     3061 caatcgccag cgcccagggt tcatgcaccc gtcttccata gggagagtgc aaaataatac
     3121 gccagtcgcc aatttcatca cggcaccgtt cgaggaccag atggcgactc cccggaacga
     3181 tgcccgtcgc gttgcgttgc tcgtcaatca acccctgaat attggcgttc gtattttctt
     3241 ctgccagcca cgggggaata gtcccggaaa agaacgcgcc atccgccagt aaatgaagaa
     3301 aatcgccgat catctcgcct aattcagccg gacgtccgtt accttcacca cgccagaagg
     3361 ggagccgggc agaacgaccc ggagcaggag tcacaatcac ctgatcgcgg gtgatttgct
     3421 ggatccgcca tgaggtagcg ccgagagtga taatgtcgct cacccgcgac tcatatacca
     3481 tctcctcatc cagttcaccc acccgccgcg aaccggcctg ttcttcacct tcgggtaata
     3541 acacgctata catgccacga tccggaatgg tgccgccgct ggtaaccgcc aacaattgag
     3601 cgccaggtcg ggcggtcaat atcccggtct ccctgttcca gaccagtttg gggcgaaaag
     3661 cagaaaaatc gccagaggga tagcgcccgg aaagcatatc cagcgtggcg tcaaaaacac
     3721 gtcttggcag atctttccac ggtgcggcac ggcgtacgcg ggagtaccat tcgtctacct
     3781 gtaatgcatc catcgccgcg gcggcaacgg tttgctgcgc aaggacgtcg agtggattat
     3841 gcggcggtgt caggttttcc agcctgcctg cgaacataca ctctacaatg actgcggaat
     3901 cgactaagtc acgccgggta cgagggaaaa acagcccttt agatacaccg ccaacctgat
     3961 gtccggcgcg accaatgcgt tgtaacccac tggcaacaga aagcggcgtt gccacctgaa
     4021 tcaccagatc caccgcgccc atatcaatcc ccagttcgag actggaggtt gcgaccacgc
     4081 agcgtaattc acccgatttc agcgcctgtt cggtgattgc tcgttgttct ttagagacgg
     4141 agccgtggtg tgagcgggca ataaaaacgt cgctactttg tacatggtta gaggttgcgc
     4201 cggaggtcga ctcgaaatgg gccgtatcaa cggcgataga cggggaacgc tgtaagcgtg
     4261 cggcgtaaag ctcatttaat cgtgccgtca gtttttccgc cagcccacgt gaattagtaa
     4321 agacaatggt cgagcgatgg cgcaacactt catcaaggat acccgtttca atatatggcc
     4381 agatggagcc ttcccggccg gcatggctgt cttcgccggt gccgctggcg accgatgaga
     4441 catcatccat attggcgacc ggtacgacaa ttcgtatctg cggatggcgc attgcgggcg
     4501 ggttgactac cgtaaccggg cgatcgccac caagaaatgc tgccacatcg ctggctgagc
     4561 gcacagtggc agaaaggcca attcgctgtg ctgaggtgtg gagcagcgca tcgagccgct
     4621 ccagacttaa cgccagatgc gcaccacgtt tactgcccgc taccgcgtgg acttcatcaa
     4681 taattaccgt ttcgacgccg cgtagcgttt cgcgcgcgcg ggaggtcagc atcagataga
     4741 gtgattcggg tgtggtgatc agaatatccg gcggattacg ggtgagtttg ctgcattcct
     4801 gtgcaggcgt atcgccagta cggatcccta cgcgaagatt gacttccgtt tcgccgcgcc
     4861 gccgccgttc atcggcaata cccttcaacg ggagctgcaa gttgcgctga acgtcggtgc
     4921 ccagagcttt tatcggtgaa atatagagga tgcgtgaggt tttacgctta tgcgcctcgc
     4981 gggtatcttc gccgccttcg cggaagagcc gatcgagggc gtagagaaat gctgccagcg
     5041 ttttcccgga gccggtcggt gcaatcacca gcgcatgttc gcttcgcgcc gccacatgcc
     5101 aggtttgcga ctggacagcg gtcggctgtt taaaggcgcg aagaaaccag tcgcgggtcg
     5161 ccggtgaaaa cacgtccggc aggagcgatg aagggtctgg attatctgcc attaacgcta
     5221 ttccccgtga atatcatgct tgatgcgtgg aatagcctca ctatagatgc ctgaatcatt
     5281 gcatgtcatc aggcatcgac tcgattacac ctcttcggcg gcaggtagcg gccagcctcc
     5341 cagacgtttc cagcggttga cgatttcaca aaacagcaca gcagtgcgtt cggtgtcgta
     5401 cagcgcggag tgcgcctggg tgctgtcgaa gtccatgcca gcggtctggc aagcctttga
     5461 caatacggtt tgtccgagtg ccagcccggc cagtgcagca gtgtcaaaag tggcgaaagg
     5521 gtggaacggg ttacgtttca gtgaggcgcg ttctgcggcg gccatcataa agctgtgatc
     5581 aaaattggca ttgtgcgcca ccataatggc gcggttacag ccgctcgctt taataccttt
     5641 acgtacaact ttaaaaattt cgtgcagcgc ctcgtattcg ctgaccgcgc cgcgatcggg
     5701 atcgttcggg tcaatgccgt tgaaggcgag ggcctctggc tgcaaatttg cgccgacaaa
     5761 tggttcgacg tggaaatgta atgtggtgtc cggcatcagc cagccttgtt catccatttt
     5821 cagggtgatg gcggcaatct caagcagcgc atcggttttg gcgttaaatc cggctgtttc
     5881 aacatcgatc acaacaggat aaaaaccacg aaaacggtcg cacagaccgg taagttgagc
     5941 gttatcggac atctgattct cttaatacgg ataaattgca gcgcgcatta tgacaaatat
     6001 tacttgtcga tgcagtaaag atgcgggcgc gatgagttca cgcccggcag gagattagtt
     6061 gcccagaccg cgaccggcgt ctttctcttc gattaactca attttgtaac cgtccggatc
     6121 ttccacaaac gcgataaccg tagtaccgcc ttttaccgga cccgcttcac gggtcacgtt
     6181 acccccgttt tgacggattt tttcgcacgc ttcagcggcg ttatctacgc taagcgcgat
     6241 gtgaccataa gcagtgccga gttcgtattt atccacgccc cagttgtagg tcagttcaat
     6301 caccgcttct tcggtttccg ggccgtagcc aacaaacgcc agtgagtatt tgtattccgg
     6361 gttttcgctg gtacgcagca gtttcatgcc cagcactttg gtataaaaat cgatggagcg
     6421 ttgcagatcg ccaacgcgca gcatggtatg aagaagacgc atctttttat cctcaaaatg
     6481 gctaacagat tcaaaatgtt gttttagtat agcggcggct ttacgccgct cgcaatgttg
     6541 gattacaacg tcgggtaatc ggtatagcct tccgcgccgc caccgtagaa actttcggca
     6601 cgctgtgggt taagctcagc tttgcgctgc aagcgggcga ccagatccgg gttcgcaatc
     6661 cagtcacgac caaatgccac cgcatcaatt aaccctttgc cgatcagcgt ttcagctttt
     6721 tctactgtgt atgcacctgc gccgataatc ggaccgtgga aacgggcgcg tactttttcg
     6781 cggaacgcat cagtatacgg ttcacccccc gcccaatctg gttctgacat atgcagataa
     6841 gcaatgccgc gtttacccag ttgttcaatc agatacagtg catcggcttc ttcattcgcg
     6901 ccgttatccg tgttctggaa agtaccgatt ggcgaaacgc gaatgccaat gcgatcggca
     6961 ccccattctt caatcccggc atcgaccact tccagtacca gacgtgcgcg attttccacg
     7021 ctgccgccgt actgatcggt acgatggttt gaagaaggag aaaggaactg atgcagcaaa
     7081 taaccgtgag cagagtggag ctctaccaga tcaaaaccgg cttcacgcgc gttagcaatg
     7141 gcctgacgga aatcattgac gatacctgga atctcttcca gttcaagcgc acgcggcatg
     7201 gatgtttcaa cacggatcgc ctgaccattt tcatcgcgca gagaagtacg tgttcccgcg
     7261 ctaagtgctg aaggcgctac cggtgcctga ccgccaggtt gcaggctggc gtgagaaatg
     7321 cgtccggtgt gccacagctg cacggccata tgaccatttt cagcatgaac gccagcggtg
     7381 atttttttcc atgcggcaat ttgctccgga ctatggatgc caggcgcacc tgcatatcct
     7441 tttgcctggg cagaaatttg cgtggcttca ctaataatca aaccggcact ggcacgttgg
     7501 cgatagtatt ccgccatcaa cggggtagga atgtcacccg gttcaatact gcgcagacgc
     7561 gtcagcggtg ccataaaaat acggtttgcc gccgtgatcg cgcccacttt cagtggggaa
     7621 tacagttttt cagatgacat aaaggctccc ggtcgtctgg tgataaaaaa tgcctgctaa
     7681 acggcaggcg tcgcaataat gttttttaca tgggccagcg cgttttccag tggctcgaaa
     7741 ctgcgcgaaa ttttggcctg cagattcgca cccagccata gtgcgtaaag cacttgtgcc
     7801 tgttgtagcg gttcgccaca aaaggttaaa caatggttct cacggccatt ttccagcgcc
     7861 tgagagagca gggcgatcac gccgcgagca cctttatcca tcgcgctgcg catatcttct
     7921 gacagatcgc acacttcggc agagagtttt actgtcaggc aaccactgat ggttccatgt
     7981 tggcaaaact ggttcagtgt ttgctggtaa taagccagta tgcggtcgcg gtagttacct
     8041 tcgccggatt gcagcaactc agtcagtcgc tggtgatatg cggcgtaatg acgctcaagc
     8101 atggcaacgc caaacgcttc tttagagcga aagtagtgat agaaggaccc tttcggcact
     8161 tcagcggttt ttagtaattc gcttagcccc atcccggtga atccacgttg caggcaaagt
     8221 tgctcgcccg tcgccaggag atgttcgcga gtatcatgtt cggtgtgttt gttcatgcgt
     8281 tggagtgtag tagaccagtc ggtctagtgc aagcaaagtt gctactttgg acattaacag
     8341 cttcaataat ggtaataatc ttatctgagg agggcgaagt ggcggagcaa ttagagttct
     8401 ttcccgtcca gagcccgtgc cggggaattt gccagtctga cgaacgcggg ttttgtcgcg
     8461 gctgttttcg tagtcgtgat gagcgtttta actggaataa aatgagcgat ggcgaaaaac
     8521 aagaggtgct gcgcctttgt cggcagcgat taatgcgcaa attacgcgca aataagccag
     8581 cgtcatcaga cgaaccggag caaccatcac tcttttgaac ctaatcttgc gtatactcct
     8641 ctcatatctg ataagaggaa gtgattatgg ttcagcgtat tactattgcg ccgcaaggcc
     8701 cggagttttc ccgttttgtg atgggctact ggcgattgat ggactggaat atgtccgccc
     8761 gccagctggt cagttttatt gaagagcatc tggatctcgg cgtgaccacc gtggaccatg
     8821 ctgatattta tggtggctat cagtgcgaag cggcgtttgg cgaggcactg aaactggcac
     8881 ctcacctgcg tgaacggatg gaaatcgtca gtaaatgcgg tatcgcgacg accgcgcgtg
     8941 aagaaaacgt cattggtcat tacatcactg accgcgatca catcattaag agcgccgaac
     9001 agtcgctaat taatctcgcg accgatcatc tggatctgct gttaatccac cgaccagacc
     9061 cgttaatgga tgccgatgaa gtggcggacg cgttcaaaca tctgcatcag agcggcaaag
     9121 tgcgtcattt tggcgtatcg aactttacgc ctgcgcaatt tgccctgttg caatcacgtc
     9181 tgccgtttac ccttgccact aatcaggtgg aaatatcccc ggtgcatcag ccgttactgc
     9241 tggatggcac gctcgaccaa ctacaacaac tgcgtgttcg tccgatggcg tggtcctgcc
     9301 ttggtggtgg tcgtctgttt aatgatgatt atttccagca gctgcgtgat gaactggctg
     9361 tggtggcaga ggagttaaac gcgggctcga ttgaacaggt ggtttacgcc tgggtattac
     9421 gtttaccatc gcagccgctg ccaattatcg gttcaggtaa aattgagcgc gtacgggcag
     9481 ctgtcgaagc agaaacactg aaaatgaccc gtcaacaatg gtttcgtatc cgtaaagcgg
     9541 cactggggta cgacgtaccg taagcacttt taggaatagc cgccgttcaa aaatgtgtca
     9601 ctggtttaca cttattcagg aatgcacaat gaacggaggt cctatgaaac gttttagtct
     9661 ggctattctg gcgctggttg ttgcaaccgg cgcacaagct gccagtgaaa aagtcgagat
     9721 gaacctcgtc acgtcgcaag gggtagggca gtcaattggt agcgtcacca ttactgaaac
     9781 cgataaaggt ctggagtttt cgcccgatct gaaagcatta ccccccggtg aacatggctt
     9841 ccatattcat gccaaaggaa gctgccagcc agccaccaaa gatggcaaag ccagcgccgc
     9901 ggaatccgca ggcgggcatc ttgatccaca aaataccggt aaacatgaag ggccagaagg
     9961 tgccgggcat ttaggcgatc tgcctgcact ggtcgtcaat aatgacggca aagctaccga
    10021 tgccgtcatc gcgcctcgtc tgaaatcact ggatgaaatc aaagacaaag cgctgatggt
    10081 ccacgttggc ggcgataata tgtccgatca acctaaaccg ctgggcggtg gcggtgaacg
    10141 ctatgcctgt ggtgtaatta agtaattaag aggccagcgt accttgcggt ggtgcttgct
    10201 caagttgcga aagcgagcag tacagccgcc agacaattgc cgccagctca cgggcggcag
    10261 gttgatgatg acgggcaagg ctgtcgcaaa tccgctgtaa ttcttcaagt gtggccgcca
    10321 gtgatttttg ctgaacgcca cgctcactca tcactccccg caacagtgaa atacagttat
    10381 cccgtactcg cgataacgga tcggaacgcg attcccagtc gcgcaattgc cagacaacat
    10441 gagaacagtt cagcagcact acaccccagc gtaataacca acggcgagcc agcgcatcct
    10501 ggctgttact caactgactg acgtgatgat aagtgagcga ttcaaattcg ctttcactca
    10561 gtgttggatg gcggcttagc tgatcgacaa aatcccggcg cagcgcgcga atatggcggc
    10621 ggcttttacg agcatccgat cctggacgca gaatggcgaa cgctagccaa gccagcgcaa
    10681 cgccaacgat ttttgccaga ttatcgttaa gaaaatcagc aaaatcatac accgggggat
    10741 tagtgacagc gataaacgaa cccataaaaa caatcagttg cccccacagt gcggcaaatt
    10801 tgggcatctg caatttaaga agctgcattg tcgccagcag tggaaagaga aacagtaaaa
    10861 attgccacag atcgctaatc tggaccatca ggccaaattt gaccacaaag ctgaatagcg
    10921 aaagtaacac cagcgtgcgc atcagcagtg acaacgactt aaacggtgct gcgacggcgg
    10981 agtagagtac gcagctaatt gctgccagcg ttaatgcatt ggcaccggca tcccattgcg
    11041 atgcaatact ccatgcgcca atcatcatca acgtacaaaa tgtacgcagc ccgctccaca
    11101 tagcttcggc gttgtcggta tgacgagcca gagcggatgt ccgtgcgagt ctggcatgat
    11161 catctacacc gctttgcaga cgatgtaatt cctgactact ttgcagataa aggcggcaaa
    11221 aataacgtag tcgctgccag aaggcgacgt gccggtagtc ggcgacgttg gtcgggcgta
    11281 gcggggcgat aatacgtgcg acggtgtaag catctgttta cgaactggcg agcgccgtca
    11341 gcaattgttc gagaatttct cgcgtggcac ctggcggtga gggccagttg agcaacatac
    11401 gtcgcaggct ggagatgaca ctggtcatac gtaattgctg gtggagtagc gcattaaggc
    11461 gcgcgttttg ctggcgaaaa cgatagtggc tccagaaagc ctggatacgc agcaaattca
    11521 tggtcagtat ctgcccaatc accccctcat gtgctgcacg aatggcatcg tttgtttcag
    11581 gctgccagag taaactggca tgttccagta atcgggcgtg catgtttttc aatgcggtta
    11641 aaagggctgt agcatcggaa ctgctaggca ggatcatcat catcatgccg ccgcacaaaa
    11701 taccgactat cacctcgcaa acgcgcgcct gagcgatatc ccacagctgg ctggcctcag
    11761 taatattaac catcggaaag gcgatgatgg cagccgtgta gcccgccagt tgaaatgcat
    11821 acgcgacgtt attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca
    11881 tgctcaatag aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa
    11941 tggctcccag taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg
    12001 tgggaaagct aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat
    12061 aataggcaac cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt
    12121 gcgccagcgt ggccttgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta
    12181 ttgacctacg gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt
    12241 atccagcgca aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt
    12301 cacatccgga ataaggcttg agtcactttc aacgctttga tcataaatcg cccgaccgat
    12361 actggaaacg tgaccccgta acgttttgtt atcactatag agggtaattt gtgctggtgc
    12421 accttcgcgg atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg
    12481 gctgtcgaca agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt
    12541 gaggttagtc acccaaccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg
    12601 tgtctgtttc agcgtggcct gtgcggcatt aacactggcc tgcatcgctt taacattgag
    12661 gttagcggta tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt
    12721 cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt
    12781 cagctcggcg atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac
    12841 cagttggttg tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga
    12901 aaccttttct gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca
    12961 cagccaccat ccggcaagaa ccgcaactat cgctacaatg attgttgaga aatacttaat
    13021 tgttttaatt gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc
    13081 gcaaacagcg ataaatccat taacaaggga tgccagatgt caccggcgta gatccagcca
    13141 cgaagcaggc ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac
    13201 ggaggaaaat agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg
    13261 agcataaact tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa
    13321 tattctttaa gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca
    13381 aatgcagcaa tacatttgtt ttagcaatac aattgctgca cactattcta aaagccgcat
    13441 aatatcttag caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct
    13501 ggcacggttg gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt
    13561 aacacaaacc cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca
    13621 aattcaactg gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca
    13681 actggaagaa aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg
    13741 tattaaactg acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa
    13801 aacccgcgcg gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct
    13861 catcgcaaaa cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg
    13921 cgtgtggtca gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga
    13981 ctgccattgc tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc
    14041 tgtaccacca tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt
    14101 ttgttcattg cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca
    14161 gtagccagag aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca
    14221 gcaccgccaa ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt
    14281 acgttaacga tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa
    14341 acatcccctg acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa
    14401 acaaccaata cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag
    14461 catccctcat ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat
    14521 atgagcaaat cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt
    14581 attgatttga agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt
    14641 cacgaaaagt ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga
    14701 aatcgggccg ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt
    14761 tggcgacaat agatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg
    14821 tgtctctgaa acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt
    14881 ttggacagct tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta
    14941 aggaacaaaa cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct
    15001 ggcatgaacc gacgggcgtg gaaccacaca ctttacagat tggcgataac aatcaaattg
    15061 tggcacgcac cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag
    15121 gacaaggcgc accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc
    15181 gacgaatggt gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc
    15241 cggttggcgg ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc
    15301 aggccggtaa accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattctcc
    15361 cactgctgca aaatatgctc agcgacccat atttctcgca acctgcaccg aaaagcaccg
    15421 gacgcgaata ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg
    15481 atccccgtga tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag
    15541 ttttgttgag cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc
    15601 tactcatggc gcgtctggcg gcattactgc caggcacaga agtcaccacc accgatgccg
    15661 ttggcattag tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc
    15721 tggcgggatt accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg
    15781 gggctatttt ccctgctaac ccgtgatatc agagttaact gaatttttct taccgcgtct
    15841 gccgttacac ttggaacgtt agggagggcg tattgccctc cagaccagga aagtcttcgg
    15901 gaaatgtcta tgaaaaaact gttactgatc tgtttgccgg tgcttctcac cggctgtagc
    15961 gcgttcaatc aactggttga gcacatgcaa accgctacgc tggaatacca gtgtgatgaa
    16021 aaaccgttga cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat
    16081 caactactgc atctcaaaca gggcatttca gcgtctggtg cacgttacac tgacggaatc
    16141 tatgttttct ggtcgaaagg cgatgaagcg actgtctata aacgcgaccg catcgtattg
    16201 aataactgtc agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag
    16261 cgtcacccac tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat
    16321 cgcgcatctg cgccgtgaat acaccaaagg cgggttacgc cgtcgcgatc ttcccgccga
    16381 tccattaacc ctttttgaac gttggctctc tcaggcgtgt gaagccaaac tggcagaccc
    16441 taccgcgatg gtggtcgcta ccgtggatga acatgctcag ccttatcaac gcatcgtttt
    16501 actcaaacat tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc
    16561 acatcaaatt gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg
    16621 ccaggtgatg gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt
    16681 tcatagccgc ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat
    16741 ttctgcccgc ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg
    16801 cgaagtgcca ttgcccagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt
    16861 ctggcagggc ggtgagcatc gcctgcatga ccgctttttg taccagcgag aaaatgatgc
    16921 gtggaagatt gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt
    16981 actcctgatt ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc
    17041 ggcaaaggtg cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa
    17101 caattgcaag agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga
    17161 ctggcgcaag gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat
    17221 ttggggcatc ttgttccatt gttatgcctg aaacgcttcc agcaggcggg ccacaagccg
    17281 gttgcgctgg taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag
    17341 cgtaagctga acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt
    17401 gccccgttcc tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac
    17461 tggttcggca atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt
    17521 aaccagatga tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt
    17581 tcgttcactg agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa
    17641 cagtacggtg tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt
    17701 atcgacctga cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc
    17761 actaaagcag atggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg
    17821 aagaaaacca gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt
    17881 taccgcttcc tgaagttctt cacctttatg agcattgaag agatcaacgc cctggaagaa
    17941 gaagataaaa acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact
    18001 cgtctggttc acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc
    18061 agcggttctt tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta
    18121 ccgatggttg agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg
    18181 caaccttccc gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt
    18241 gaaaaacagt ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt
    18301 accttactgc gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag
    18361 tggaaagggg gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa
    18421 tattctcgct atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt
    18481 tcctatgcgc cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca
    18541 cacccaatac ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt
    18601 gcaaggcatt gccgccattg ataaattaca cacctgtgat gccgtgttaa gtggctatct
    18661 gggatcggcg gagcagggtg aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa
    18721 tccgcaggcg aaatattttt gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt
    18781 tgcaccgggt gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg atatcattgc
    18841 gccgaatctg gttgagctgg aaatactctg tgagcatgcg gtaaataacg tcgaagaagc
    18901 ggttctggca gcgcgcgaac tcattgcgca agggccacaa attgtgttgg ttaaacacct
    18961 ggcgcgagct ggctacagcc gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc
    19021 ctggcatatc agccgtccgc tggtggattt tggtatgcgc cagccggtag gtgttggtga
    19081 tgtgacgagc ggtttactgc tggtgaaact gcttcagggg gcaacgctgc aggaggcgct
    19141 ggaacatgtg accgctgcag tctacgaaat catggtgacc accaaagcaa tgcaggaata
    19201 tgagctgcaa gtggtggctg ctcaggatcg tattgccaaa ccagaacatt acttcagcgc
    19261 aacaaagctc tgaaatatct catttcggcc cacaacgacc tgtgggccga tggatattac
    19321 tttaagcctt ccgctgacag cgcgtcttgt acttccggac gttcagccat acgttgcata
    19381 aatgctgcaa tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga
    19441 accgtaaaca gataggcatc agcaattgta aatctttgcc cgcagatcca gtgctcatcc
    19501 ttcagtgcct cgttcacata ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg
    19561 tactcttccg gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg
    19621 atgtaattca gccattcgat ggttttatag cgggaaatac tgtttaccgg tgccagcaac
    19681 tggcggtcgg ggacgctgtc ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa
    19741 gtaccgtcat ccagcagcaa tgcaggcacc tgccccttag ggttaacggc aaagtaatcg
    19801 tcaccgtttt cgagacgttt tttcattaaa tccacactga cgagggtaaa atcctttccg
    19861 ctctcacgca gggtgatatg ggaagcgaga gagcaggcac ccggtttgta gaacaatttc
    19921 atcggtaact ccttttgtct gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa
    19981 agccgctaac gaaaagttag cggcttgtaa aagagtttcc ctgaatttta cgctacagca
    20041 gctttcgccg ctttgtctgc agcgtcatcc tgcgtcatac ggtgcagttt aggcgcggtc
    20101 agcagcatca gtactgcaat aatggcagta gcgacaccaa tctgcaagaa tacgcgacca
    20161 taaacttcca gtgacatcag cgggtcggta acgttatccg gcacagccat catacccgca
    20221 acataaccac caatcaggtt tgcaccggca gtggtcagga accagctacc cataatgaag
    20281 cccatcagac gctgcggaac gagttgtgca accattgcca gacccagacc agagatcatc
    20341 agttccccga tgctctgcag gccatagctt gcgaccagcc agcttacaga cacgatacca
    20401 gcgtcagacg cgaatttcgc acccagcggc agaatcagga acgcaccaga acacatcacc
    20461 atgccgattg caaacttggt tggcatcggc agggtatcgc ccatcttgtt atagatagcg
    20521 gccagaatcg gactaccgat gatgatccag aacgggttca gtgcctgata ctgctcaggt
    20581 tctacggcca gacccagaat ggagtgctca acgttacgaa tcgcaaagaa gttcagtgat
    20641 gtcggcatct ggctgtacag cacgaagaag atgattgctt cgagcatcag gatgaaggca
    20701 acgatcattt tacgacgcgc agcacctttc atcgcgaagg cttctttacc gaagataacc
    20761 acgataccga aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc
    20821 caggtggcga tagcgatcag tgccacaaca ccaataatgg ttagcagcag gttacggtag
    20881 ttgataggct cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg
    20941 aagttaacga tagtgatcag caggcctaca acgctcaatg caaacgcaac gctccagccg
    21001 tatttcgcgg ccagccacgg cgtagcaatc atagagaaga aagagccgat gttgacggac
    21061 atgtagtaca tggtgaatgc accgtccaga cgcgggtcgt ttttctcata gcatgtagaa
    21121 agcagagaag acgggttagc cttaaacagg ccgttaccga ccgcaatagc cgccataccc
    21181 atataaacaa taccggcgtc gtgaccagac caggcaacca gagcataacc aatcgccagc
    21241 acaatagcgc cgagcataat tacgcgttta gtacccagta ccttgtcacc taaccagccg
    21301 ccgatagcga ccagaccata aaccagggca ctaaaggaag agaaaagggt gattgaatcc
    21361 gcttcagaca tacccagttg tttaaccagg taaacagcca taattccttg taggccgtaa
    21421 taaccaaaac gttcccataa ctcaatcgag aagatgagat agaacgcctt cggttgtttg
    21481 aaagcgttca aactgacgct ttcagttggt ttttggtttg cagtggacac ttttacctct
    21541 ttttttacat cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg
    21601 caattgttat agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca
    21661 agtcctttgt aataatctgt tacatataac tgaggcgggg aattcaacca ttccgggaaa
    21721 tgttatccag cgttaaattt ttcaaaaaac gtaacggcag attttttcac tgcactgaga
    21781 ggggggtgat ggcttaacgg cgatatgttc tgctatttag cgtttttact agtgagatag
    21841 tccagtttct gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta
    21901 aatttaaatg attttaacct gatttttgca atgtttttac attgccacag catcgtgatc
    21961 ttgatcacga atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt
    22021 tatccgcaaa cggattacct gatgataacg ctaagcaatg gcatcattca cgttaaaaag
    22081 atgttttaaa agaggataaa gaaaggttat caatggggta atcggtgtta ccccttttct
    22141 tcagatgtca actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg
    22201 gggcttgcgg gcaatgcagg tataacgccc gtgcaggatc aaccaatggt ggcagtcgac
    22261 tttaaactct gccggaacca cttttagtag cttttcttct acctgttcga catttttccc
    22321 cggcgcaaat tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg
    22381 ccagccgaat gcagtgttta atacgacgtt ggctgtttta cgacctacgc cgggcagggc
    22441 ttcaagcgca gcacgatctt ccggaaccaa gccattatgc tgctccagta agatacggca
    22501 ggttttgatg atattttctg ctttgctgtt ataaagccca atcgttttga tataggtttt
    22561 aaccccttca acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt
    22621 cgccgtcgcc ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag
    22681 caattcaaaa ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag
    22741 gcgagtgagg atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt
    22801 ggcaatgcac gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg
    22861 tattttcctg ccagcatcag tcccaggcca ataaatgcac ctggaggcag catcgccagc
    22921 aggaaagggg agtcggtgtg gaaaatttcc acgcgtaata cttttgccca gctacctaac
    22981 agcgcatctg caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag tgaacccagc
    23041 acgaacatgg cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc
    23101 ggaccttttt tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc
    23161 agcggaataa aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc
    23221 tgtacagcgc tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct
    23281 ggcgtccagt gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc
    23341 gcaagtccta aacccagagc gttagtggca gtggacgtga ccgccaacag aggacaaagg
    23401 ccgagcaact ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta
    23461 atttcgctca cgctttattc tccacaggca ggaagttgag aaagttgtgc cggtaacgtc
    23521 tgagcgtaca atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg
    23581 ccggtgaact ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatctgca
    23641 ccactgattt ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta
    23701 tcgccaagcc ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta
    23761 aaatcggctc ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgcttcc
    23821 agaacggcgg ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta
    23881 cctaactctg gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg
    23941 gctggcagca cctgatcaaa taatgccttt tgttgcagac tggcctgttc agcaatcgtc
    24001 gttttggtca tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagcgct
    24061 aacgtaatgc cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag
    24121 acgcgcggac gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca
    24181 aaagccacgc cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgcc
    24241 agcgcgccga aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa
    24301 ataaagaatg cgccgagcat ggtcgcacca gacagcagat gaatttgcgg tgctgccagt
    24361 gtttctggtg agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg
    24421 aggggaatat gccagcgaat cgctttctgc catagcaacc acaggccgcc agccagccag
    24481 gcgagattta cccattgcca accagcgccc gccagaatac cgctgtagat cggatattgc
    24541 ataatctgtt caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc
    24601 gcctgactaa tgccatcaat acctaagcgt agtgtgttca tatcaccacc actggcggta
    24661 tgtccgctga aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca
    24721 tgtggcggta accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca
    24781 atcattgccg gattaaacgg gttttgcccc agaccgccat acaactgttt agcgatgatc
    24841 accgcaaaca ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc
    24901 gccagcaata agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc
    24961 ttgcgtagtt tgagtacgag agcttcggct aacagagcac taaccgatgc caacaggatc
    25021 tgaacgagag taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg
    25081 agcaacacca acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta
    25141 gctattctga ataccattta gtcctcgttt acaaccttct gctgggcggc ttttttggcc
    25201 tgagcgcggg caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt
    25261 tctgcgttag tctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg
    25321 acggcggctt tgcgcggatc aacctgttct tctggttccg cattagcctg ttgctgttcc
    25381 agtttgcgcg ctttggctcg ggcaatagcg gcttcgacgg cggctttgcg cggatcaacc
    25441 tgttcttctg gttccgcatt ag
//
LOCUS       DAZFRJ010000056        25252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000056 DAZFRJ010000000
VERSION     DAZFRJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.056"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            114..2150
                     /gene="yihQ"
                     /locus_tag="ACHKUN_002063"
     CDS             114..2150
                     /gene="yihQ"
                     /locus_tag="ACHKUN_002063"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO6660316.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            2196..3581
                     /locus_tag="ACHKUN_002064"
     CDS             2196..3581
                     /locus_tag="ACHKUN_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660317.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            3624..5027
                     /locus_tag="ACHKUN_002065"
     CDS             3624..5027
                     /locus_tag="ACHKUN_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660318.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5095..5787
                     /gene="ompL"
                     /locus_tag="ACHKUN_002066"
     CDS             5095..5787
                     /gene="ompL"
                     /locus_tag="ACHKUN_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO6660319.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(5878..7143)
                     /locus_tag="ACHKUN_002067"
     CDS             complement(5878..7143)
                     /locus_tag="ACHKUN_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660320.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(7245..8225)
                     /locus_tag="ACHKUN_002068"
     CDS             complement(7245..8225)
                     /locus_tag="ACHKUN_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO6660321.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(8233..8943)
                     /locus_tag="ACHKUN_002069"
     CDS             complement(8233..8943)
                     /locus_tag="ACHKUN_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6660322.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(9160..10983)
                     /gene="typA"
                     /locus_tag="ACHKUN_002070"
     CDS             complement(9160..10983)
                     /gene="typA"
                     /locus_tag="ACHKUN_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO6660323.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            11356..12765
                     /gene="glnA"
                     /locus_tag="ACHKUN_002071"
     CDS             11356..12765
                     /gene="glnA"
                     /locus_tag="ACHKUN_002071"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO6660324.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13084..14133
                     /gene="glnL"
                     /locus_tag="ACHKUN_002072"
     CDS             13084..14133
                     /gene="glnL"
                     /locus_tag="ACHKUN_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO6660325.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            14145..15554
                     /gene="glnG"
                     /locus_tag="ACHKUN_002073"
     CDS             14145..15554
                     /gene="glnG"
                     /locus_tag="ACHKUN_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO6660326.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            15666..15776
                     /locus_tag="ACHKUN_002074"
     CDS             15666..15776
                     /locus_tag="ACHKUN_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO6660327.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(15966..17339)
                     /gene="hemN"
                     /locus_tag="ACHKUN_002075"
     CDS             complement(15966..17339)
                     /gene="hemN"
                     /locus_tag="ACHKUN_002075"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO6660328.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(17528..18037)
                     /gene="yihI"
                     /locus_tag="ACHKUN_002076"
     CDS             complement(17528..18037)
                     /gene="yihI"
                     /locus_tag="ACHKUN_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO6660329.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(18156..18322)
                     /locus_tag="ACHKUN_002077"
                     /pseudo
     CDS             complement(18156..18322)
                     /locus_tag="ACHKUN_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18619..19251
                     /gene="yihA"
                     /locus_tag="ACHKUN_002078"
     CDS             18619..19251
                     /gene="yihA"
                     /locus_tag="ACHKUN_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO6660330.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(19633..22419)
                     /gene="polA"
                     /locus_tag="ACHKUN_002079"
     CDS             complement(19633..22419)
                     /gene="polA"
                     /locus_tag="ACHKUN_002079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO6660331.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            22783..23715
                     /locus_tag="ACHKUN_002080"
     CDS             22783..23715
                     /locus_tag="ACHKUN_002080"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6660332.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(23756..25186)
                     /locus_tag="ACHKUN_002081"
     CDS             complement(23756..25186)
                     /locus_tag="ACHKUN_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6660333.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         6141 a         6336 c         6110 g         6665 t
ORIGIN      
        1 aacagctcag cttctgtaaa gtttttatat atcagtttta tgagaatcat tttacataag
       61 cctgtaataa caggaaggca acaatctcgt tctcaactct atggagatta gttatggata
      121 cgccacgtcc acagttatta gattttcaat ttcatcagaa taacgacagt tttaccctac
      181 attttcaaca acgtcttatt ttaacccata gcaaagataa tccttgttta tggattggct
      241 caggtatagc ggatatcgat atgttccgcg gtaatttcag cattaaagat aaactacagg
      301 agaaaattgc gcttaccgac gccatcgtca gccagtcacc ggatggttgg ttaattcatt
      361 tcagccgtgg ttctgacatt agcgccacgc tgaatatctc tgccgacgat cagggccgtt
      421 tattgctgga actacaaaac gacaacctta accacaaccg tatctggctg cgccttgccg
      481 ctcaaccaga ggaccatatc tacggctgcg gcgaacagtt ttcctacttc gatctgcgtg
      541 gcaaaccgtt cccgctatgg accagtgaac aaggcgttgg tcgcaacaaa caaacctatg
      601 tcacctggca ggccgactgc aaagaaaatg cgggcggcga ctattactgg actttcttcc
      661 cacagcctac gtttgtcagc acgcagaagt attactgcca tgttgataac agttgctata
      721 tgaacttcga ctttagtgcc ccggaatacc atgaactggc gctgtgggaa gacaaagcaa
      781 cgctgcgttt tgaatgtgct gacacataca tttccctgct ggaaaaatta accgccctgc
      841 tgggacgcca gccagaactg cccgactgga tttatgacgg agtaacgctc ggcattcagg
      901 gcgggacgga agtgtgccag aagaaactgg acaccatgcg taacgcgggc gtgaaggtca
      961 acggcatctg ggcgcaggac tggtccggta ttcgtatgac ctcttttggc aaacgcgtga
     1021 tgtggaactg gaagtggaac agcgaaaact acccgcaact ggattcacgc attaagcagt
     1081 ggaatcagga gggcgtgcag ttcctggcct atatcaaccc gtatgttgcc agcgataatg
     1141 atctctgcga agaagcggca caacacggct atctggcaaa agatgcctct ggcggtgact
     1201 atctggtgga gtttggcgag ttttacggcg gcgttgtcga tctcactaat ccagaagcct
     1261 acgcctggtt caaggaagtg atcaaaaaga acatgattga actcggctgc ggcggctgga
     1321 tggctgactt cggcgagtat ctgcccaccg acacttacct gcataacggc atcagcgccg
     1381 aaattatgca taacgcctgg cccgcgctgt gggcgaaatg taactacgaa gcccttgaag
     1441 aaacgggcaa gctcggcgag atccttttct ttatgcgcgc cggttctacc ggtagccaga
     1501 aatactccac catgatgtgg gcgggcgacc agaacgtcga ctggagtctc gacgatggcc
     1561 tggcgtcggt tgtcccggcg gcgctgtcgc tggcaatgac cggacatggc ctgcaccaca
     1621 gcgacattgg cggttacacc accctgtttg agatgaagcg cagcaaagag ctgctgctgc
     1681 gctggtgcga tttcagcgcc ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg
     1741 gcgacaactg gcagtttgac ggcgacgcag aaactatcgc ccattttgcc cgtatgacct
     1801 ccgtcttcac caccctgaaa ccttacctga aagaggctgt cgcgctgaat gcgaagtccg
     1861 gcctgccggt tatgcgcccg ctgttcctgc attacgaaga cgatgcgcac acttacaccc
     1921 tgaaatatca gtacctgtta ggtcgcgaca ttctggtcgc tccggtgcat gaagaaggcc
     1981 gtagcgactg gacgctctat ctgccggagg ataactgggt ccacgcctgg acgggtgaag
     2041 cgttccgggg cggggaagtt accgttaatg cgcccatcgg caagccgccg gtcttttatc
     2101 gcgccgatag cgaatgggcg gcactgttcg cgtcgttaaa aagcatctaa tcacatccgc
     2161 ccgtggggaa actcatgggc aaagggagaa catcgatgag tcacatcaca acggaagatc
     2221 cagcaacttt acgcctgccc tttaaagaga aactctctta cggtattggc gacctggcct
     2281 ctaacatcct gctggatatc ggtacgcttt atcttttgaa gttttatacc gacgttctgg
     2341 ggctgccagg cacctatggc ggcattatct ttttgatttc aaaattcttt actgcgttta
     2401 ccgatatggg taccggcatt atgttggatt cccgacgcaa gatcggtcca aaaggtaagt
     2461 tccgtccttt tattctgtat gcgtcattcc cggtcacctt actggcgatc gccaactttg
     2521 tcggcacacc gtttgatgtc accggtaaaa cggtgatggc cactattctg tttatgcttt
     2581 acggactgtt tttcagcatg atgaactgct cctacggcgc aatggttccc gctatcacca
     2641 aaaaccccaa cgaacgcgcc tcactggcgg catggcgtca gggcggcgct acgctgggcc
     2701 tgctgctgtg cacggtggga ttcgtgccag tgatgaatct tatcgagggt aatcagcaac
     2761 ttggctatat cttcgccgcc acgctgtttt cactgttcgg cctgctgttt atgtggatct
     2821 gctactcagg cgtgaaagag cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc
     2881 ctggcctgtt gcaatctttc cgcgcaattg ccggtaaccg cccactgttc attctgtgta
     2941 ttgccaacct ctgcacctta ggggcgttta acgtcaagct cgccattcag gtctattaca
     3001 cccagtacgt acttaacgat cccatcctgt tgtcgtatat gggatttttc agcatgggct
     3061 gtattttcat cggcgtgttc ctgatgcctg gcgcagtcag acgttttggt aagaagaagg
     3121 tctatatcgg cggcctgctg atttgggtgc tgggcgatct gctcaactat ttcttcggcg
     3181 gcggctcggt cagctttgtg gcgttctcct gcctggcgtt cttcggctca gcgtttgtta
     3241 acagcctgaa ctgggcgctg gtttccgaca ccgtcgagta cggcgagtgg cgcaccggcg
     3301 tgcgttcgga aggcacggtc tacaccggct tcaccttctt tcgcaaagtg tctcaggcgc
     3361 tggctggttt cttccccggc tggatgctga cgcaaattgg ctatgtgccg aacgtcgcac
     3421 aggctgacca caccattgaa gggttacgcc agttgatctt catctaccca agcgcactgg
     3481 cggtagtcac cattgtggcg atgggttgct tctacagcct gaacgagaag atgtatgtcc
     3541 gcattgtgga agagatagaa gcccgtaaac gcacggcgta ataataataa acgacgccct
     3601 gcggggcgtt ataaggagtg attatgtctg accataatcc actgacatta aaactgaatc
     3661 tgcgggaaaa aatcgcctat ggtatgggcg acgtcggttc gaatttaatg ctctgcatcg
     3721 gtactctgta tctcctcaaa ttttataccg atgaattagg gatgcctgct tactatggcg
     3781 ggattatctt tctggtggcg aagtttttta ccgcgtttac cgatatgctc accggatttt
     3841 tactcgactc gcggaaaaat attgggccta aaggcaaatt tcgtcccttt attttatatg
     3901 cagcagttcc ggcggcgtta attgcaacgc tccagtttat cgccaccacc ttttgtttgc
     3961 cggttaaaac gacgattgcc accgcgctgt ttatgatgtt tggactttca tacagtctga
     4021 tgaactgctc gtatggtgcg atgatcccgg caattaccaa aaacccgaat gagcgcgcgc
     4081 agctcgcggc ttaccgccag ggtggtgcaa ccatagggct attgatttgt accgtggcgt
     4141 ttattccgtt gcagtcgctt ttttctgact caaccgtcgg ttatgcctgt gcggcactta
     4201 tgttctccat tggcggcttt atttttatga tgctgtgcta cagaggcgtc aaagagcatt
     4261 atgtggacac agcgccaacc ggacataaag ccagtattct caaatctttt tgcgcgatat
     4321 ttcgtaatcc accattgctg gttttatgca ttgctaacct gtgtactctg gcggcattta
     4381 atatcaaact ggcgattcag gtctattaca cccagtatgt gctgaatgat attaatttat
     4441 tgtcgtggat gggattcttc agtatgggat gcatcctcgt cggcgtatta ctggtgccag
     4501 taactgtaaa atgttttggt aaaaaacagg tttatttagc tggcatggtg ctatgggcgg
     4561 tgggtgatat actgaattat ttctggggaa gtaactcttt cactttcgtc atgttctctt
     4621 gtgtcgcctt ttttggcacg gcctttgtaa acagcctgaa ctgggcactg gtaccagata
     4681 ccgtagatta cggtgaatgg aaaaccggta ttcgtgccga aggttctgtt tataccggtt
     4741 ataccttctt tcgtaaaatt tctgccgcac ttgctggctt cttgccaggc attatgctga
     4801 cgcaaattgg ttatgttccc aacatcgcgc aaagcgatgc gacattacag ggtttgcgtc
     4861 agctcatttt tatctggcct tgtgcactgg caattattgc tgcattgacg atgggattct
     4921 tttacacact caatgaaaaa cggtttgcat tgattattga ggaaatcaac caacgcaaaa
     4981 ataaagagat ggcgacagaa gaaaaaacgg cttccgtaac gttataaatg tcaatagccg
     5041 ctatttccat cctggtgggt ggcggcctcc ctacgtttaa aaaatggact tattatgaaa
     5101 aagattaatg caataatttt attatcttct ctgacctctg cctcggtatt tgctggcgca
     5161 tacgtcgaaa acagggaggc atataatctt gcctccgatc agggcgaggt tatgctacgt
     5221 gtgggttata acttcgatat gggcgcgggt attatgttaa ccaataccta cacctttcag
     5281 cgagaagatg aactaaaaca tggctataat gaaattgaag gttggtatcc tttattcaaa
     5341 ccaaccgata aattaaccat ccagcccggt ggcttaatta atgataagag tatcggttcg
     5401 ggtggtgcag tgtatctgga cgtcaactat aaatttgtac catggtttaa tctgacagta
     5461 cgaaatcgct ataaccataa taactatagt tcaacagatt tgagcgggga actggataat
     5521 aatgacacct atgaaattgg cacctactgg aattttaaaa tcaccgataa attttcctat
     5581 acatttgagc cacattattt catacgcgta aatgacttta atagcagcaa tggaaaagat
     5641 catcactggg aaatcactaa taccttccgt tatcgcatta atgaacactg gctgccttat
     5701 ttcgaattac gttggttaga tcgtaatgtc gaaccgtacc accgcgagca aaaccagatt
     5761 cgtatcggga cgaagtattt cttctgatgt gaaactgccg gatgcggcgt aaacgcctta
     5821 tccggcctac ggttgatacg gttttcgttg gccggattaa gcacatccgg ccattaatca
     5881 agcttcagcc gccagcatct ctttcttacg ttggctaatt cgcttaacca gcattacgga
     5941 aaccaccgca ccacagaatg cgaagcaggc cataatgcca aacacgattt tgtagccaat
     6001 aatccccgga tttaaatcca gaatgtagcc atacagactg aagcagaaca tcgccggagc
     6061 gtaaccaata aagctaccca acgccatcgc cgcgcctgtt ttattttcag caatttttgc
     6121 ttcgccgata ggtgcaaaaa atacggctcg ctgtgtaaag actatcgcgc caaagcccag
     6181 cgtacatgcc atccctaaat agaccggcat actttcgtgc ggcagcataa tcaacagtac
     6241 gagcgcagcg gtactgatga taaaggtgta gcatagatat ttactcggcg atttcaggat
     6301 cttatctgaa atcatgccac caatcggtcc accaatcatt ttcagacagt attggttaat
     6361 gatgccgtaa gcccccacca gcgcaacggg caatagatag atgtttttca ggaatggaat
     6421 gaagaatgtc aggccacagt aaaccgcgta aacgaagaag acgttaaaag cgataagcca
     6481 gatggttttg tctctcagca ccgaggtcat actggtgttt tgcgatgctc cgtcttcttt
     6541 tttcacctca acggacggtg cctcttcttt gtcattcagg acaaagaaaa taataatgcc
     6601 tacggcaatc acaataaggg aatagaacca gatgcctgct ttaaaaccta ataagccact
     6661 gccaaaccag gtaaatactg ccaacgcaga aaatgccacc acggtatcga caatgccacg
     6721 ccctgtttcg aagaagccaa acaaccgacc ttgttgttcg ctattgccca atcgacttac
     6781 cgacttgagc aagaccggcc agttcatcat gtcgcaagta acgccaaata gcgcccagac
     6841 aaagaggatg ccccaatacc ccggcattgt cgtcaaataa acacccagta atcctgtcgc
     6901 aatgagtgag aatgacatgg tgtatctgcg cggtagttta tcggcaaaat aaatagacag
     6961 aaaaaagccc actgtggtga caaatgagtt taccgacata gcattaccaa tttgaccatt
     7021 ggtcaaatgg aaatattcct gcatcgggat ataaaacgca tctttcagcg acggtaattt
     7081 ataaattgta ccgccacaca atgttaatag actaaataac gcccatttct ttttcgtgag
     7141 cataacatcc cctaagcgat ttttaaaatc actgttttaa aggcaataag ataccgacca
     7201 ttcatatgga atggttttat taactgcgta tgagttaaaa cttattagtg gttcagaaga
     7261 tggtttgcct cttgtttgat ttgttgtagt acgttacgca catgatttat ctgattaatt
     7321 gcatcttctt tttctttacg taaccgcgca tccagtaaat gattttctgg caatggtgtt
     7381 tcactcatct ggcgataagg gatccacgga ttatcatctt cgtcttcaaa gcggaacgcc
     7441 ggcgcatagt aatcaacctc ttcttccagg ccatatgccg tcacctgcgg ctcatcatca
     7501 cccaggcata taaggtgcgt taataacgct ttgaacggca tatcaccctg acctgaaata
     7561 caggctttat gacccaggcc acccggttct ttaacgatca aggcatcttt gatatggacc
     7621 tgggtaatat gcggcgccat cgtttttaaa gcgtcgatgg gatgctcatt tgcattgatc
     7681 atgttcgcaa aatcaaacag taaggaaaga gattccatct cactttcttt gaccagcgac
     7741 accagctcat ggctttttaa atcttcatgc tgttcgatcg taaaagtcag gccgctgtcc
     7801 tgatacgttt cccgtacata ggcaatgtcg ttagcgatga tcgataatac gtcgcgcaaa
     7861 ttaccttcat aacgtggata aaaacgtacg gacgatgccc cagttttcaa cgcaatggcg
     7921 acggcttcgt caatagatgc cttatctgaa gcgctggttt caatatgaat atcaaggttc
     7981 agacggcggg ctttatcccc aaaggcagag agttctttat cgtccatatt accaagagaa
     8041 aaacgctcgc catccagaac atggattttg acgccacgta gattattttc acttgcgata
     8101 tcaagtaaat cagcaggcaa cactctttcc agccgcatat ttaaatgaaa ggcataagca
     8161 tgtaaataaa gaggaagagt gtcgacacgt tgtagaatct ttcttgcgtt atttattgtc
     8221 accataaaac acctacagat taatgatgag gtctacggcc tcaaatcgcc aatattcaat
     8281 atctatctgg acaatattgc cttgctggtc ggcgcgatgc ttttcaatta agatcgctgg
     8341 catacccgca gaaccgccaa gatacttgct gatatcgccc ggcattctga cgggtttgaa
     8401 aatcaatttt tttactaccg tctctttttg gtagcacttt tcccagactg cagaaaatga
     8461 gtggttttca agttgttcaa taaaacccgg agcaacttca ggattaatat atgtttcgtg
     8521 atagaaaact ttatgtcctt ccagcgcccc ccagccggtg atgcgataga gttcagttga
     8581 tggtgtcact gcaatcagtg gcgcgagcgt cttgggaata tcgctggtac ggtttttctc
     8641 ggtaaacccc caggaaggct ctcgtccttg ctcaattgcg gcccgctgaa agctcgccga
     8701 caactccgga ctgtaattaa atcgcggctg ggtcacgaac caccccttac gatccttacg
     8761 gaagattttg gattctgcct cgaggttcaa caacgcctgg cgcagcgtca tacgtttaat
     8821 gcccagcagt tctcccagct ctctttctga agggagtttt ccacccggcg tggtgatgcc
     8881 atctttcaac caccgatcca gtttctcttt tgcattttct acggtggatt gattctccgc
     8941 cattgtttaa tccttttggt ttaaaccaga tgaagcatct tacgcttacg ccaaaaaaga
     9001 aagcaattaa cggcgtatga ctttgcagat aattgtgaca aagctctcta ttgacgtaat
     9061 cccagactta taagggagat actcgcattg gtgtgcgctc acgacaggta aaaaaaaacc
     9121 tgccagcgat ggcaggtttt ttataactaa aaaacgaaat taatcgtctt tcggtgcgcg
     9181 gttggcgcgg cgacgatcgt tttccgtcag gtgacgttta cgaatacgga tagaggtcgg
     9241 agtcacttct accagttcgt catcatcgat gaactccaga gcttgttcca gagtcatgcg
     9301 gataggcgga accagaacaa cggcttcgtc agtaccggaa gcacgcatgt tggtcagttt
     9361 cttaccggtc aggcagttta ccgtcaggtc gttagagcgg ctatgaatac cgataatctg
     9421 accttcgtaa acttctgcac cgtgaccgag gaacagctta ccgcgatcct gcagaccgaa
     9481 cagcgcgaac gcgaccgctt taccctgacc gttagagatc agtacgccgt tctgacgctg
     9541 acccacttca cccggacgta cgtcgtcgta gtggctgaag gtggagtaca gcagaccagt
     9601 accggaagtc atggtcatga actcagaacg gaagccaatc agaccacggc ttgggatcac
     9661 gtagtcgaga cgtacgcggc ctttaccgtc tggattcatg tttttcaggt cgcctttacg
     9721 ttcgcccagc gcctgcatta cagaaccctg atgctgttct tcaacgtcca gagtcacgtt
     9781 ttcatacggc tcttgtttac gaccgtcgat ttcacggaag ataactttcg gacgggatac
     9841 cgccagttcg aaaccttcac gacgcatgtt ttcgatcaga acagacaggt gcagttcgcc
     9901 acgaccagaa acgcggaacg catcggcgtc ttcggtttct tctacgcgca gcgcaacgtt
     9961 gtgtaccagt tctttgttca gacgatccag gatctgacga gacgttacga acttaccttc
    10021 tttaccgcag aacggcgagg tgttaacgca gaagaacata gaaacggtcg gctcatcaac
    10081 ggagagtgcc ggcagcgctt caacgttttg cgtgtcgcaa acggtgtcag aaatgttcag
    10141 ttcgccaagg cccgtgatcg caacgatatc gccagcttcc gccagatcgg tttcgatacg
    10201 ttccagaccg aggtggccca gcactttacc gactttcgcg ttgcgggttt tgccttcgct
    10261 atcgatgata gtgacctgct ggttcggctt cactttaccg cgcttgatgc ggccaatgcc
    10321 gataacgcca acatagctgt tgtaatcgag ctgagaaatc tgcatctgga acggaccgtc
    10381 aaggtcaacg tccggcgcag gaacgtggtc aacaatcgcc tggtacagcg gggtcatgtc
    10441 ttccgccata tcttcgtggt ccagacccgc gataccgttc agcgcagaag cgtaaacgat
    10501 cgggaagtcc agctgctcgt cggtcgcgtc gaggttaacg aacagatcga atacctgatc
    10561 cacaacccaa tcaggacgcg cgccagggcg gtcaactttg ttgataacaa caatcggctt
    10621 caggccgtaa gcaaacgctt ttttggttac gaagcgcgtt tgcggcatcg ggccgtcaaa
    10681 tgcgtcaacc accagcagca ctgagtctac catggacatt acacgttcaa cttcaccacc
    10741 gaagtcggcg tgccccgggg tatcaacgat gttgatacgg taatcattcc atttgatagc
    10801 ggtgtttttc gcgaggatgg taatcccccg ctctttctcc aaatcgttgg agtccatcac
    10861 gcgctcttgg gtttcggcac gagagtcgaa cgtaccggat tgttggagca gcttgtctac
    10921 cagggtggtt ttaccatggt ctacgtgcgc gatgatggcg atattacgca atttttcgat
    10981 cacaactttg cctcaggcat tagaaatagc gcgttattgt acacggatta atcgcactac
    11041 aaaacaggat cacaaacatc ctccgcaaac aagtattgca gagtcccttt gtgatcgctt
    11101 tcacggagca taaaaagggt tatccaaagg tcattgcacc aacatggtgc ttaatgtttc
    11161 cattgaagca ctatattggt gcaacattca catcgtggtg cagccctttt gcacgatggt
    11221 gcgcatgata acgcctttta ggggcaattt aaaagttggc acagatttcg ctttatcttt
    11281 tttacggcga cacggccaaa ataattgcag atttcgttac cacgacgacc atgaccaatc
    11341 caggagagtt aaagtatgtc cgctgaacac gtactgacga tgctgaacga gcacgaagtg
    11401 aagtttgttg atttgcgctt caccgatact aaaggtaaag aacagcacgt cactatccct
    11461 gctcatcagg tgaatgctga attcttcgaa gaaggcaaaa tgtttgacgg ctcctcgatt
    11521 ggcggctgga aaggcattaa cgagtccgac atggtgctga tgccagacgc atccaccgca
    11581 gtgattgacc cgttcttcgc cgactccacc ctgattatcc gttgcgacat ccttgaacct
    11641 ggcaccctgc aaggctatga ccgtgacccg cgctccattg cgaagcgcgc cgaagattac
    11701 ctgcgttcca ctggcattgc cgacaccgta ctgttcgggc cagaacctga attcttcctg
    11761 ttcgatgaca tccgtttcgg atcatctatc tccggttccc acgttgctat cgacgatatc
    11821 gaaggcgcat ggaactcctc cacccaatac gaaggtggta acaaaggtca ccgtccggca
    11881 gtgaaaggcg gttacttccc ggttccaccg gtagactcgg ctcaggatat tcgttctgaa
    11941 atgtgtctgg tgatggaaca gatgggtctg gtggttgaag cccatcacca cgaagtagcg
    12001 actgctggtc agaacgaagt ggctacccgc ttcaatacca tgaccaaaaa agctgacgaa
    12061 attcagatct acaaatatgt tgtgcacaac gtagcgcacc gcttcggtaa aaccgcgacc
    12121 tttatgccaa aaccgatgtt cggtgataac ggctccggta tgcactgcca catgtctctg
    12181 tctaaaaacg gcgttaacct gttcgcaggc gacaaatacg caggtctgtc tgagcaggcg
    12241 ctgtactaca ttggcggcgt aatcaaacac gctaaagcga ttaacgccct ggcaaacccg
    12301 accaccaact cttataagcg tctggtcccg ggctatgaag caccggtaat gctggcttac
    12361 tctgcgcgta accgttctgc gtctatccgt attccggtgg tttcttctcc gaaagcacgt
    12421 cgtatcgaag tacgtttccc ggatccggca gctaacccgt acctgtgctt tgctgccctg
    12481 ctgatggccg gtcttgatgg tatcaagaac aagatccatc cgggcgaagc catggacaaa
    12541 aacctgtatg acctgccgcc agaagaagcg aaagagatcc cacaggttgc aggctctctg
    12601 gaagaagcac tgaacgaact ggatctggac cgcgagttcc tgaaagccgg tggcgtgttc
    12661 actgacgaag caattgatgc gtacatcgct ctgcgtcgcg aagaagatga ccgcgtgcgt
    12721 atgactccgc atccggtaga gtttgagctg tactacagcg tctaatagtt gaagttgtac
    12781 tacccggcgc aacaacgccg gaggttcaaa ccaggcccat ctggcgtaat tgttgcagcc
    12841 agtttgaaca cggacagcgc gcagaacccg gagcgtacac tggtacgtga ggagttcgag
    12901 cactgcccag gttcaaaatg gcaaataaaa tagcctgatg ggactggttt ttagttgccg
    12961 tggaaatttc agcccatctc tgcatgggct tttttctccg tcaattctct gatgcttcgc
    13021 gctttttatc cgtaaaaagc tataatgcac taaaatggtg caacctgttc aggagactgc
    13081 tttatggcaa caggcacgca gcccgatgct gggcagatcc tcaactcgct gattaacagt
    13141 attttgttaa tcgatgacaa cctggcgatc cattacgcca accctgccgc gcaacaactg
    13201 ctcgcccaaa gctcccgcaa attgtttggt acaccgttac cggaactgtt gagctacttc
    13261 tcattaaata tcgagctgat gcaagaaagt ctggaggcgg ggcaaggttt taccgataac
    13321 gaagtgacgc tggtcatcga cgggcgctcg catatccttt ctgtgacggc ccagcgtatg
    13381 ccggacggca tgatcctgct ggagatggct ccgatggata accagcgccg cttaagtcag
    13441 gaacagctac agcacgccca gcaggttgct gcccgtgatt tagtgcgcgg cctggcacat
    13501 gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc agctgctcag caaagcgtta
    13561 cctgacccat cactactcga atataccaaa gtgattatcg aacaggcgga ccggctgcga
    13621 aatctggtcg accgtctgtt ggggccgcag ctgcccggta cgcgcgttac cgaaagtatt
    13681 cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg aactgccgga caacgtgcgg
    13741 ttgattcgtg attacgatcc cagcctgccg gaactggcgc acgacccgga tcaaattgaa
    13801 caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc tggggccgga aggcggtgaa
    13861 atcattctgc gtacccgcac cgcgtttcaa ctgaccttac acggcgagcg ctaccggctg
    13921 gcggcgcgga ttgatgtgga agataacggg ccgggcattc cgcctcattt gcaggatacg
    13981 ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg ggcttggctt atccatcgct
    14041 cgtaatttga ttgatcagca ttcaggcaaa attgaattta ccagttggcc agggcatacc
    14101 gagttctcgg tttacctgcc tatcaggaaa taaaggtgac gtttatgcaa cgagggatag
    14161 tctgggtagt cgatgacgat agttccatcc gttgggtgct tgaacgtgcg ctcgctgggg
    14221 caggtttaac ctgtacgacg tttgagaacg gcgcagaagt gctggaggcg ctggcgagca
    14281 aaacgccgga tgtgctgctt tcagatatcc gtatgccggg aatggacggg ctggcgctgc
    14341 tcaagcagat taaacagcgc catccaatgc ttccggtcat cattatgacc gcacattccg
    14401 atctggatgc tgccgtcagc gcctatcaac aaggggcgtt tgattatctg cccaaaccgt
    14461 ttgatatcga cgaagcagtg gcgctggttg agcgcgctat cagtcattac caggaacagc
    14521 agcagccgcg taatgttcag cttaacggcc caacgaccga tatcatcggc gaagcgccag
    14581 ccatgcagga cgtgttccgt attatcggtc ggctttcgcg ttcttctatt agcgtgctga
    14641 ttaacggcga atccggcacc ggtaaagaac tggtcgctca tgccctgcat cgccacagtc
    14701 cgcgcgccaa agcgccgttt atcgcgctga atatggcagc tatcccaaaa gatttgatcg
    14761 aatcagaact gtttggccac gagaaaggcg cgtttactgg cgcgaatacc attcgtcagg
    14821 ggcgttttga acaggccgat ggcggtacat tattcctcga cgaaattggt gatatgccgc
    14881 tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg tcagttttac cgcgttggcg
    14941 gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc cactcaccag aatctcgaac
    15001 agcgagtgca ggaaggtaag ttccgtgagg atctgttcca ccgcctgaac gttatccgcg
    15061 ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc ccgtctggcg cgccattttt
    15121 tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt actgcatccg gaaaccgaag
    15181 ctgctctgac gcgtctggcg tggccaggca acgtgcgcca gctggaaaac acctgccgct
    15241 ggctaacggt gatggccgcc gggcaggaag tgttgattca ggatttgccc ggcgaactgt
    15301 ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc ggacagctgg gcgacgcttc
    15361 ttgcgcagtg ggcagacaga gcgctgcgtt ccggtcatca aaatctgctt tccgaagcgc
    15421 agccagagct ggagcggacg ttactgacga ccgcgttgcg acatacgcag gggcataaac
    15481 aggaagcggc gcggctactc ggctggggcc gcaacaccct gacgcgtaag ttaaaagagc
    15541 tggggatgga gtgattcaca gcttgtgtgt aaagattgat tattgagcgc aaattgctgg
    15601 tattttacgc tttactgttc cgataagttc agtatgatct tgcccggaaa acggggagag
    15661 tcattatgct ggaatcaata attaatctgg tatcgagtgg cgcagttgac agccacacac
    15721 cgcaaactgc cgttgctgcc gtgctgtgtg ccgcaatgat tgggctgttt agttgagttt
    15781 cagattgctg atgaagtgct ctttgttcat gccggatgcg gcgtgaacgc cttatccggc
    15841 ctacaaaagc aatattttca acaggttttg taggcctgat aagcgtagcg catcaggcaa
    15901 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
    15961 actatttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
    16021 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
    16081 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
    16141 aagtaatcag cgaagtgcaa atcccacagt ttctcaatag gggcgtaatc cagacggaag
    16201 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
    16261 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
    16321 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
    16381 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
    16441 gccaccgcca gctcgtcatc cggacgggca aagtgatcca taccgataaa ctgatagccc
    16501 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
    16561 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
    16621 aagacactca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
    16681 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
    16741 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
    16801 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
    16861 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
    16921 gcatcggcat tgaactggaa gttttcgcgc agcagcttca tcaggcggct gatttgcgct
    16981 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
    17041 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
    17101 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
    17161 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
    17221 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
    17281 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    17341 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    17401 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    17461 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    17521 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    17581 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    17641 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    17701 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    17761 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc acgcccaatg
    17821 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    17881 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    17941 gcttgcgatc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    18001 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    18061 tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    18121 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    18181 gccgccaaca caccctgttt ttcctttagc cggaacaatc cgtgttgatt ccatttccgt
    18241 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    18301 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    18361 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    18421 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    18481 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    18541 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    18601 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    18661 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    18721 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    18781 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    18841 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    18901 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    18961 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    19021 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    19081 cgtaaagcgc aattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    19141 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtggaca agctgcggca gaaactggat
    19201 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    19261 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    19321 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    19381 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    19441 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    19501 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    19561 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    19621 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    19681 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    19741 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    19801 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    19861 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    19921 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    19981 gccctgctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    20041 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    20101 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    20161 ttgctcgctg gtgacggttt ccagtggcaa actaaacact tctgccgccg ttgcccggtg
    20221 gatatctttt ccttccgcga atgcggtcag caagcctttg tcacgcgaaa gatgcgccat
    20281 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    20341 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    20401 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    20461 cccggttttc gggttgatca tcagcggcag cttgtcggtg taggtcgatt tcagcttcgc
    20521 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    20581 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    20641 tttttcaaag agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    20701 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    20761 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    20821 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatctgg
    20881 ccacattttc agatgcaact gcaaggtgac atcagcatct tcggcggcgt aacgtccggc
    20941 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    21001 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    21061 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagctc
    21121 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    21181 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgcgag agatttgatc
    21241 gggcgcgtca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    21301 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    21361 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    21421 gatggtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    21481 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    21541 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    21601 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacgtcggt
    21661 tttaatcgtc gccagctggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    21721 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    21781 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    21841 tacgccagga atgttatcgg aggagtcacc catcagcgcc aggaaatcga tgatcagttc
    21901 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    21961 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctgat
    22021 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    22081 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagggg
    22141 ttcgatttgt gctcgcagat cgttcggcat tggcgggcga tgtgatttgt aatgttcaaa
    22201 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    22261 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    22321 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    22381 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    22441 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    22501 gaaaatcttt tgtagatctt ctggatcgct cgcaaatcgt catgtggata actttgtgca
    22561 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tatttgtctt
    22621 tgtttttcat gcagttaaaa ataaagccat ccctaataat tgagataatg ggattttttc
    22681 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcgttt
    22741 tctgctaaaa tctgcgcctt gcgcttcttt agcgcactgt ctatggctaa ccttttgaat
    22801 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    22861 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    22921 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    22981 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    23041 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    23101 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    23161 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    23221 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtaaa
    23281 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    23341 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    23401 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    23461 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    23521 ttcgatatgt taagcggtaa actgacgcga attgtcgttc atgtggattt acagcctatt
    23581 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    23641 tggctgaact cactgtggca ggaaaaagac caacttctga cgtcgttgat gtcatcacag
    23701 cggcaggaca agtaaatttt gctcgcctct gaatgagcag aggcgagtga gtattttaat
    23761 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    23821 caactttacc cggtgtataa cgcagttgca gcgaggcggt attgttgtca atcgtgatta
    23881 actgaaacat ctgacctaag gtcatcatcc cgctgatttg tttatcagcc cgcttttgcg
    23941 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    24001 taagcggtaa cttcacatca aaattgagcg atttaatatc tttgtttgtg gatgatgaag
    24061 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    24121 tccattttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    24181 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    24241 cagcaagctg cttctgcatt gcaatgttat actggatgat aaattggcgc accgctgagg
    24301 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    24361 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    24421 ccagcatggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    24481 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    24541 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    24601 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    24661 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    24721 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    24781 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactaagttc acttttgccg
    24841 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    24901 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    24961 tatcgaatgt catttgcatt tgaaaacggg aattaaaaaa gcctttgtca aagtttttat
    25021 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    25081 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    25141 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    25201 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc gg
//
LOCUS       DAZFRJ010000057        25248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000057 DAZFRJ010000000
VERSION     DAZFRJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.057"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..842
                     /locus_tag="ACHKUN_002082"
     CDS             <1..842
                     /locus_tag="ACHKUN_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6660334.1"
                     /translation="ANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTF
                     AALGASWGIPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIV
                     TDGMPIMIKDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNK
                     EAAIYSGINVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLG
                     GTSLNGGYGTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKR
                     KRS"
     gene            864..1826
                     /locus_tag="ACHKUN_002083"
     CDS             864..1826
                     /locus_tag="ACHKUN_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6660335.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            1823..2869
                     /locus_tag="ACHKUN_002084"
     CDS             1823..2869
                     /locus_tag="ACHKUN_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO6660336.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(2961..4310)
                     /gene="accC"
                     /locus_tag="ACHKUN_002085"
     CDS             complement(2961..4310)
                     /gene="accC"
                     /locus_tag="ACHKUN_002085"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO6660337.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4321..4791)
                     /gene="accB"
                     /locus_tag="ACHKUN_002086"
     CDS             complement(4321..4791)
                     /gene="accB"
                     /locus_tag="ACHKUN_002086"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO6660338.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5657..6487)
                     /locus_tag="ACHKUN_002087"
     CDS             complement(5657..6487)
                     /locus_tag="ACHKUN_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660339.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(6524..7498)
                     /locus_tag="ACHKUN_002088"
     CDS             complement(6524..7498)
                     /locus_tag="ACHKUN_002088"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO6660340.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            7650..9590
                     /gene="csrD"
                     /locus_tag="ACHKUN_002089"
     CDS             7650..9590
                     /gene="csrD"
                     /locus_tag="ACHKUN_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO6660341.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9895..10938
                     /gene="mreB"
                     /locus_tag="ACHKUN_002090"
     CDS             9895..10938
                     /gene="mreB"
                     /locus_tag="ACHKUN_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO6660342.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            11004..12107
                     /gene="mreC"
                     /locus_tag="ACHKUN_002091"
     CDS             11004..12107
                     /gene="mreC"
                     /locus_tag="ACHKUN_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO6660343.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            12107..12595
                     /gene="mreD"
                     /locus_tag="ACHKUN_002092"
     CDS             12107..12595
                     /gene="mreD"
                     /locus_tag="ACHKUN_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO6660344.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            12604..13197
                     /gene="yhdE"
                     /locus_tag="ACHKUN_002093"
     CDS             12604..13197
                     /gene="yhdE"
                     /locus_tag="ACHKUN_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO6660345.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            13187..14656
                     /gene="rng"
                     /locus_tag="ACHKUN_002094"
     CDS             13187..14656
                     /gene="rng"
                     /locus_tag="ACHKUN_002094"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO6660346.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            14724..18524
                     /gene="yhdP"
                     /locus_tag="ACHKUN_002095"
     CDS             14724..18524
                     /gene="yhdP"
                     /locus_tag="ACHKUN_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO6660347.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            18680..20125
                     /gene="tldD"
                     /locus_tag="ACHKUN_002096"
     CDS             18680..20125
                     /gene="tldD"
                     /locus_tag="ACHKUN_002096"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO6660348.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(20253..21182)
                     /gene="aaeR"
                     /locus_tag="ACHKUN_002097"
     CDS             complement(20253..21182)
                     /gene="aaeR"
                     /locus_tag="ACHKUN_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO6660349.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            21365..21568
                     /gene="aaeX"
                     /locus_tag="ACHKUN_002098"
     CDS             21365..21568
                     /gene="aaeX"
                     /locus_tag="ACHKUN_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO6660350.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            21576..22508
                     /gene="aaeA"
                     /locus_tag="ACHKUN_002099"
     CDS             21576..22508
                     /gene="aaeA"
                     /locus_tag="ACHKUN_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO6660351.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            22514..24481
                     /gene="aaeB"
                     /locus_tag="ACHKUN_002100"
     CDS             22514..24481
                     /gene="aaeB"
                     /locus_tag="ACHKUN_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO6660352.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            24573..24845
                     /locus_tag="ACHKUN_002101"
     CDS             24573..24845
                     /locus_tag="ACHKUN_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO6660353.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24901..25164)
                     /gene="yhcN"
                     /locus_tag="ACHKUN_002102"
     CDS             complement(24901..25164)
                     /gene="yhcN"
                     /locus_tag="ACHKUN_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO6660354.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5952 a         6156 c         6858 g         6282 t
ORIGIN      
        1 ccgccaacaa cattactaat attattcttc aggtttcgat aattgcaatt accgcctatg
       61 gaatgactta tgtacttttg cttggcgata ttgatttatc ggtcggttca accattgcat
      121 tgattggcac atttgctgcg cttggggctt catggggaat tccatttatt ttattggtcc
      181 cattatccat tattgcagca ctggctctgg ggatgataaa tggtggcttg acggctatag
      241 cggggatccc gtcgtttatt gtcactgttg ctactatggg gatcttccgt ggtatcgctt
      301 atatagtgac cgatggtatg cccatcatga ttaaagatga tgcctttctg gcactgggga
      361 atggagaatt tctctatata cctattccaa tctggatatt aatcatctta ttgctgatta
      421 accacttcat tcttactaaa acaacctttg gacgaaaaat ttatatcact ggtggtaata
      481 aagaagcggc aatttattca gggataaatg tcacccgctt gaaaattaaa gtctttatga
      541 ttacagccgt gctggcaggt atcagcggga tgatcctcgc ctcacgccgc tattccgggc
      601 aaccgaattc cgcgttgagc tacgaactgg atgctattgc tgcggctgta ctgggaggca
      661 ccagcctgaa cggcggctat ggaaccgtgg tggggacggt gatcggcgca ttaactatcg
      721 gcgttattaa taatggcatg aacttaatga atgttcccta tttttaccag atggtggtta
      781 aaggattagt cattcttgta gccgtttatt ttgacgtaag aaataaaaga aaacgcagct
      841 aatcatttca aggaattaat catatgagta aggtcttcac aattggagaa attctggttg
      901 aaattatggc gagtaaaatc ggccagccat ttgatcaacc aggtatctgg aacggccctt
      961 atcccagtgg tgcgcccgct atttttatcg accaggtgac acgtcttggc gttccgtgcg
     1021 gcatcattag ctgtgtgggg aatgatggct ttggtgatat taatatacat cgtctggctg
     1081 ctgacggtgt ggatatcagg gggatttcag tcttacctct ggaagctacc gggagtgcct
     1141 ttgttactta tcataactct ggcgatcgcg attttatttt taatattaag aatgccgctt
     1201 gcggtaaatt atcggcgcag catgttgatg aaaatattct gaaggactgt acccattttc
     1261 atattatggg ctcttccctg ttttcgtttc atatggtaga cgcagtaaaa aaagcggtaa
     1321 ctatcgttaa agcaaatggt ggtgttattt ctttcgaccc taatattcgt aaagagatgc
     1381 tcgatattcc agaaatgcgt gacgccctcc actttgttct tgagttaacg gatatctata
     1441 tgcccagcga gggcgaagtg ttgctcttgt cgccacattc gacgccggag cgtgcaatcg
     1501 caggttttct tgaagaaggt gttaaagagg tgattgtgaa gcgcggcaat caaggtgcca
     1561 gttactactc cgcgaatgag cagtttcatg ttgagagcta tccggtggaa gaagttgacc
     1621 cgaccggtgc gggagactgc tttggtggcg cgtggattgc ctgccgccag ctagggtttg
     1681 acgcccatcg agcgctgcaa tatgccaatg cctgcggtgc gctggcggta accagacgtg
     1741 gcccaatgga gggaacgtct cgccttgcgg aaatcgaagc gttcatccag cgccatgaca
     1801 tgtccattcg ggaagcagca caatgaaaaa cgaaatttgg tcgtggattg gcgatctctc
     1861 gcaggttgcc ggaattaaac actatgagtt acgtagtggc agagcaaagg gtacagaagc
     1921 gtttgatgtc cgtactggtg cgggactggc atttacggtg gtaaaagatc gcgcgctgga
     1981 tattgcatgg gccagctaca aggacacggc gctttctttt atcaccccca acggcattgt
     2041 cgcccccgct ttttttgagt ctcagggcaa cggattttta cgtagctttt acgcggggtt
     2101 actgactacc tgcggactta gctacattgg cacgccttgt gaggatgaag gagaaacgct
     2161 ggggttacac gggcgtttgg ctgcgactcc agcagaagag gtggggtatc gcacagaacg
     2221 gaccgacgac ggcattgaat ttgtcatcaa tggcaaagta cgggaaacgc gactgttcgg
     2281 cgaaaatctg acgctggaaa ggaccattcg ctgccgttat ggcgaaaatg tgctgcgcat
     2341 tgaagacaag gtgactaatc atggtttcac ccgccagccg ctgcaaattc tttatcactt
     2401 taattacggc tggcctttat tatcgccaca ggcagaaatc ttgctgtcgg ctaaaagcgt
     2461 aacgccacga actccgcatg cggcggaagg gttaaccagt catctggaga tttgtacccc
     2521 acagcccggt tttgacgagc aggtttacta tctcacgttg aatagcgaca gtcaggggat
     2581 gagtaaagtt gctctggtga acgccgaact ggggtgggga atttacgaga aattcgacac
     2641 catgcagcta cccaacttta tccagtggaa aaatttaggc gcgggtgagt atgttatggg
     2701 actggaggtc agtaatagtt ttcctgacgg tcgtgataaa gaacgagcgc aggggcgtct
     2761 gccctttatt gagccagggg aaacaaagaa atactgcttt gagctgggaa ttgttgatgg
     2821 cgatgctgaa atgagcgcgt tgaaagcgga aattgcaggc taccgttagc taacggcaat
     2881 aaaaacgccg ggcagcgtta acaggcccgg cgttgattac gttctttgct aagcgcgatg
     2941 aaaacaccgc gcgtcacaaa ttatttttcc tgaagaccga gttttttctc cagatagtgg
     3001 atgttagtgc caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg
     3061 ttggttttga taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc
     3121 acgtcacggt tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt
     3181 acggtgtagc ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg
     3241 aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga
     3301 cattccaccg catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg
     3361 gcagcgatac gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg
     3421 tgttctacct gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg
     3481 aactcgaaag tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg
     3541 ccgatgtagc gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc
     3601 tggtggcggc gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg
     3661 tcagccagta cctgaatctc gacgtggcga ggattttcca ggtatttctc catgtaaacc
     3721 atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt
     3781 tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc cgccgccgga ggctttgata
     3841 atcaccggat aaccaatgcg tttagcaatg gcacggtttt tatccatatc gtcgcccaac
     3901 gggccgtcag aacccggtac gcaagggacg cccgcttttt tcatcgccgc gattgcggat
     3961 actttgtcgc ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag
     4021 cgttcaacct gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt
     4081 gctactgcgc cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt
     4141 actgacggag cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg
     4201 cgatccgcgc tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga
     4261 atacgcaatg caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg
     4321 ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat
     4381 tgctttcacg gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac
     4441 gatgcacagg gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc
     4501 cgggcttggg gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat
     4561 ttccgctgct gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg
     4621 agctggctgc tgcattattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc
     4681 aggagctgca cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat
     4741 gcctgattct tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt
     4801 ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg
     4861 ccccgacagc tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc
     4921 cccagagtgt caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg
     4981 caaaaaacaa aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc
     5041 gtcagggagg acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg
     5101 gtcttatcag ggaagataat caacagctaa catgtaaata accttcaaca ccgtgtaatt
     5161 tgcaacaagc cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt
     5221 aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc
     5281 acagaccaca aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc
     5341 ggttgctggg gattacccaa ttttcactgc attgtctgga tagatgttaa tgctaaagca
     5401 cgcaaaatta accgattgac aatgctcagc attaaatagg ttcgagtgat taatttgtag
     5461 tcaaacaaaa tcagattatt ccccccattt tgcagaaggt aaactgtttt gtcaggcggc
     5521 agtgcggcta tctgatgctt tcagaatttg ccacctgaca ataaaaaagc cgatttggta
     5581 gcaaaatcgg cttcggtgag tttgcagttt tcatcacata ctccgacaag ctttttcaag
     5641 catgacggag gttgcgttaa tgcaaggttt ctacccaggg ataaaagtga ccgttacggc
     5701 ggatgaatat ctgtgttgtc agaggatggt catcctcacg gaagcattgt tttgttagct
     5761 gtcgtaatgc actgagattt gctggctgat cattgtgggt gaaaaacagt tttcctctgg
     5821 agggaacggt cactaacagg tcgccgcatt caggatcggg tgattgcacc caaagatcgt
     5881 caatgagcat cagactgggc tcataagtac cccctgcgga gataacggca acttttccat
     5941 cgtttgcgcc tgtatcaacg atttcagccg taacttccgg caatatttgc ttcaaattac
     6001 tgatggcttg ttcgcggtca atggtcactt cgtagcgcat cagatagcgc atggtttcgc
     6061 taccatcttc agcaaacaag ataattaact catcgttaaa gtcttcccag gcaaaatcca
     6121 gcgaggcgtt gaggacctct ggatcagact catactggct aagctgttgg cgaacggtat
     6181 ccagccaggc tttttcttta atcaccggaa caatacagcg cccggtcggc agcatttctg
     6241 caagctgttt cagcatatct gcagccactt cttgcagcgg gtctggtgcg gcttgataac
     6301 gtttgtaggc atcatcaagg gtgatttcca ccgtttggtg acccgtatca atgcgcatca
     6361 atttgtggtc agtcgaaatc atgactttcc agtttggatg atgttgtaca acttcctgac
     6421 caaggtgttc gacgaaagct gtccggctaa ccgcttttcc cgttaaccga ttgaataatg
     6481 atgtcaaaat gagttccctc tcttattatt ccttgctaaa tggttagtta accttcacca
     6541 gcgtgcgacc ctggatctgg ttattaatga tggcctcggc aaagttcggt gcctctgaca
     6601 gagatatctc ttttgccgcc tgggtataga atgattccgg taaatcggcg accagtcgct
     6661 gccaggcttg tgcgcggcgt tctggtggcg tcattactga atccacccct tgcaaacgga
     6721 cattacgcag aataaatggc atgaccgtgg ttggcagagt aaaaccaccc gccagaccac
     6781 aggcagccac gcagccgccg taattcattt gcgccagcac ttttgccagc actttgtcgc
     6841 caacagtgtc aattgcccca gcccagactt gtttttccag aggacgggat tcggcaaact
     6901 catcacgagg gagaatacga ctggcaccta aacttttcag atattcatgg gtactttcgc
     6961 gaccggaaac ggcaacgacc tgataaccca acttatgcag cagcgccacg gcggtactgc
     7021 cgacgccacc actggcaccc gtcacgacaa tctccccgtc ctgcgggcga acaccggcat
     7081 cttccagcgc catcacacac agcatggcgg taaaaccggc agtaccgata atcattgctt
     7141 tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc acctttcact cgcgcctgct
     7201 ccgccagccc accccagtgg ttttcaccaa cgccccagcc agtgagtaac acctcctgac
     7261 cggcatgaaa acgcggatct tcgctggtgc gtacagttcc ggcaaaatcg atcccaggaa
     7321 tcatcggaaa attacggatg atttttccct taccggtaat cgccagcgca tctttatagt
     7381 tcaggctcga ccagtgaaca tcaaccgtga catcgccctc cggcaggcga ctttcgtcaa
     7441 gtgtctgtac cgatgcgaga gttttgccgt cctgctgttc taaaagtaac gcctgcataa
     7501 gtggtcctca tgtgcatgat ggattggaaa ataatttctg aagactatac tcgctaatgg
     7561 aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa tctgatttgc tagtatgccc
     7621 gcttcctcac tatcggagtt aacacaagga tgagattaac gacgaaattt tcggcctttg
     7681 ttacgctgct caccgggtta acaatttttg tgactttgct gggctgttcg ctaagtttct
     7741 acaacgcaat tcagtataag tttagtcatc gcgttcaggc ggtggcgacg gcgattgata
     7801 cccaccttgt gtcgaatgac ttcagcacat taaggccaca aattaccgaa ttaatgatgt
     7861 cggcagatat cgttcgtgta gacctgctcc atggtgataa acaggtttat accctggcca
     7921 gaaatggtag ttatcgtccg gttggctcca gcgatctgtt tcgcgaactg agtgttccgt
     7981 tgataaagca tccggggatg tcgctgcgtc tggtttatca ggatccgatg ggcaactatt
     8041 tccattcttt aatgactacc gcgccgctca cgggggcgat tggctttatc attgttatgc
     8101 tcttcctggc ggtacgctgg ttacaacggc aacttgccgg gcaagaattg ctggaaaccc
     8161 gggctactcg tatcttaaac ggtgagcgtg gctctaatgt gttgggaacc atctatgaat
     8221 ggccgcccag aaccagcagt gcgctagata cgctgcttcg tgaaattcag aacgcacgcg
     8281 aacaacacag ccgtcttgat acgctgatcc gctcttatgc cgcccaggac gtgaaaaccg
     8341 gcctcaataa ccgactcttt ttcgataatc agttagcaac gttactggaa gatcaggaga
     8401 aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc ggatttcaat atgttgagcg
     8461 atacttgggg gcacagccag gttgaagaac agttcttcac tctgacgaat ctgctgtcga
     8521 catttatgat gcgctaccct ggcgcactgc tggcgcgtta ccaccgcagt gattttgctg
     8581 cgctgttacc gcaccggacg ttaaaagagg cagagagcat cgccggtcag ttaatcaaag
     8641 ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga cgatatgatc cacattggta
     8701 tctgcgcctg gcgtagtggt caggataccg agcaggtaat ggaacatgca gagtctgcca
     8761 cgcgtaatgc gggattgcag ggcggcaata gctgggctat ttacgatgac tcgttgcctg
     8821 aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga gcaaatgctc agtcgcggcg
     8881 gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg tcaggttcat catcgcgaac
     8941 tcatgtgccg catcttcgat ggtaatgaag aggttagctc ggcggagtat atgccgatgg
     9001 tcttgcagtt tggcttatcg gaagagtatg accgtctgca aatcagccgt cttattccac
     9061 tattgcgtta ctggccagag gaaaatctgg cgattcaggt taccgttgag tcgctgattc
     9121 gcccgcgttt tcagcgttgg ctgcgcgata cgttaatgca atgtgaaaaa tcacaacgaa
     9181 aacgcataat tattgaactt gcagaggccg atgtaggtca acatatcagt cgtttacaac
     9241 ctgttattcg tttagtgaat gctttagggg tacgggtagc cgtcaaccag gctggtttga
     9301 cgctggtaag caccagttgg atcaaagaac ttaatgttga gttactcaag ctccatccgg
     9361 ggctggtcag aaacattgag aagcgaacgg agaaccagct gctggttcaa agcctggtgg
     9421 aagcctgctc cgggaccagc acccaagttt acgccaccgg tgtgcgttcg cgaagcgagt
     9481 ggcagaccct gattcagcgc ggtgttacag gcgggcaagg ggattttttc gcgtcctcac
     9541 agccacttga tactaacgtg aaaaaatatt cacaaagata ctcggtttaa cctgccgttt
     9601 aatctgtttt cacgtagaat aacgcgcgct gcgtctcatg ggagtgtgct tgtctgctcg
     9661 ccagattgtt gcagcacata tgcagatgaa tgaccttacg cggttgcaaa caggcgagga
     9721 atgctgctga tgcattaagc ctttctggac tcaggcagag atttgtaaca aaggaaacga
     9781 actgcactaa ttttcaccgt agcagatgat ttttgcgcct tgtcgctgct gcgtgtggtt
     9841 ggtaaagtaa gcggattttc ttttccgccc cagctttcag gattatccct tagtatgttg
     9901 aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg acctgggtac tgcgaatacc
     9961 ctcatttatg taaaaggaca aggcatcgta ttgaatgagc cttccgtggt ggccattcgt
    10021 caggatcgtg ccggttcacc gaaaagcgta gctgcagtag gtcatgacgc gaagcagatg
    10081 ctgggccgta cgccgggcaa tattgctgcc attcgcccaa tgaaagacgg cgttatcgcc
    10141 gacttcttcg tgactgaaaa aatgctccag cacttcatca aacaagtgca cagcaacagc
    10201 tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg ttggcgcgac ccaggttgaa
    10261 cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc gtgaagtctt cctgattgaa
    10321 gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt ctgaagcgac cggttctatg
    10381 gtggttgata tcggtggtgg taccactgaa gttgctgtta tctccttgaa cggcgtggtt
    10441 tactcctctt ccgtgcgcat tggtggtgat cgtttcgacg aagctatcat caactatgtg
    10501 cgtcgtaatt acggttctct gatcggtgaa gccaccgcag aacgtatcaa gcacgaaatc
    10561 ggttcggctt acccgggcga tgaagtccgt gaaatcgaag ttcgtggccg taacctggca
    10621 gaaggtgttc cacgcggttt taccctgaac tccaacgaaa tcctcgaagc actgcaggaa
    10681 ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg aacagtgccc gccggaactg
    10741 gcttccgaca tctccgagcg cggcatggtg ctcaccggtg gtggcgcact gctgcgtaac
    10801 cttgaccgtt tgttaatgga agaaaccggc attccagtcg ttgttgctga agacccgctg
    10861 acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga tcgacatgca cggcggcgac
    10921 ctgttcagcg aagagtaatc ggatgcaggc aggggaaatg tctgtttacc ctgcctggtc
    10981 tgatacgaga atacgcataa cttatgaagc caatttttag ccgtggcccg tcgctacaga
    11041 ttcgccttat tctggcggtg ctggtggcgc tcggcattat tattgccgac agccgcctgg
    11101 ggacgttcag tcaaatccgt acttatatgg ataccgccgt cagtcctttc tactttgttt
    11161 ccaatgctcc tcgtgaattg ctggatggcg tatcgcagac gctggcctcg cgtgaccaac
    11221 tagaacttga aaaccgggcg ttacgtcagg aactgttgct gaaaaacagt gaactgctga
    11281 tgcttggaca atacaaacag gagaacgcgc gtctgcgcga gctgctgggt tccccgctgc
    11341 gtcaggatga gcagaaaatg gtgactcagg ttatctccac ggttaacgat ccttatagcg
    11401 atcaagttgt tatcgataaa ggtagcgtta atggcgttta tgaaggccag ccggtcatca
    11461 gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa actgaccagt cgcgtgctgc
    11521 tgatttgtga tgcgacccac gcgctgccaa tccaggtgct gcgcaacgat atccgcgtaa
    11581 ttgcagccgg taacggttgt acggatgatt tgcagcttga gcatctgcca gcgaatacgg
    11641 atattcgtgt tggtgatgtg ctggtgacct ccggtctggg cggtcgtttc ccggaaggct
    11701 atccggtcgc ggttgtctct tccgtaaaac tcgataccca gcgcgcttat actgtgattc
    11761 aggcgcgtcc gactgcagga ctgcaacgtt tgcgttatct gctgctgctg tggggcgcgg
    11821 atcgtaacgg cgctaacccg atgacgccgg aagaggtgca tcgtgttgct aatgaacgtc
    11881 tgatgcagat gatgccgcag gtattgcctt cgccagacgc gatggggcca aagttacctg
    11941 aaccggcaac ggggatcgct cagccgactc cgcagcaacc gacgacagga aatgcagcta
    12001 ctgcgcctgc tgcgcccaca cagcctgctg ctaatcgctc tccacaaagg gctacgccgc
    12061 cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg gcaatagtgg cgagctatcg
    12121 tagccaggga cgttgggtaa tctggctctc tttcctcatt gcgctgttgc tgcaaatcat
    12181 gccctggccg gataacctga ttgttttccg gccaaactgg gtgttactca tcttgttgta
    12241 ttggatcctg gccttgcctc atcgcgtaaa tgtgggcaca ggttttgtga tgggtgccat
    12301 actggatctg atcagcggct cgacgcttgg cgtacgcgta ttggcgatga gcatcattgc
    12361 ttacctggtg gcgctgaaat accagctttt ccgcaacctc gcattatggc agcaggcgct
    12421 ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt ttctgggcag agtttttagt
    12481 gattaacgtc tctttcagac cggaagtgtt ctggagtagt gtagtcaatg gggtgctctg
    12541 gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag tttgcagtgc aataaaggtt
    12601 tctatgactt ctctgtattt agcttccggt tctccgcgtc gtcaggagtt acttgcgcaa
    12661 cttggcgtga cctttgaacg tattgttacg ggcattgagg agcagcgtca gccgcaggag
    12721 agcgcgcagc agtatgttgt gcgtctggcg cgcgagaaag cacaggcggg tgtcgcgcaa
    12781 acggcgcagg atctcccggt gctgggtgcg gatactatcg ttatcctgaa tggagaagtg
    12841 ctggagaaac cgcgcgacgc agagcatgcg gcgcagatgt tgcgcaaatt atcgggtcag
    12901 acccatcagg tgatgacggc agtggcgttg gccgacagcc agcacattct cgattgcctg
    12961 gtggtcaccg atgtgacttt cagaacgtta acagacgaag acatcgcggg ctatgtcgcc
    13021 agcggtgaac cgttagataa agcaggtgca tacggtattc aggggctggg tggctgtttt
    13081 gtcaggaaga taaatggcag ctatcacgcc gtagtcggct taccgctggt tgaaacgtat
    13141 gaattattaa gtaattttaa cgcactgcgt gagaaaaggg ataaacatga cggctgaatt
    13201 gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat attgatggcg gtattctgca
    13261 ggaaattcat attgaacgtg aggcgcgacg cggaatagta ggcaatatct acaagggtcg
    13321 tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta gatattgggc tggataaagc
    13381 cgcgtttctt catgcatccg atatcatgcc gcacaccgaa tgtgtggcgg gtgaagaaca
    13441 aaagcaattc acggtgcgcg acatctcgga actggttcgt caggggcaag atctgatggt
    13501 gcaggtggtg aaagatccgc ttggcactaa aggtgcgcgc ctgaccaccg atatcacgct
    13561 gccttctcgc tatctggtgt ttatgccagg ggcttctcac gttggggttt cccaacgtat
    13621 tgaaagcgaa tcagaacgtg aacgcctgaa aaaagtggtc gcagagtatt gcgacgagca
    13681 gggcgggttt atcatccgta ccgcagcgga aggggttggc gaggctgaac tggcctccga
    13741 tgccgcttat ctgaaacgcg tctggaccaa agttatggag cgtaaaaaac gcccgcagac
    13801 ccgttatcag ctgtacggcg aactggcgct ggcgcagcgt gttctgcgtg atttcgccga
    13861 cgccgaactg gaccgcattc gcgttgactc acgcctgact tacgaagcat tgctggagtt
    13921 tacctcggag tacattcccg agatgaccag caagctggag cattacaccg gacgccagcc
    13981 gattttcgat ctctttgatg tcgaaaacga aatccagcga gcgctggaac gcaaagtaga
    14041 actgaaatcc ggtggttatc tgattatcga ccagaccgaa gcgatgacca ccgtggacat
    14101 caataccgga gcgtttgtcg gtcatcgcaa tctggacgac accattttca ataccaatat
    14161 tgaagcgacg caggctatcg ctcgccagtt acggttgcgt aatctgggtg ggattatcat
    14221 tattgatttc atcgatatga ataatgaaga tcaccgccgc cgcgtgctgc actcgctgga
    14281 gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat ggtttttcgg cgctggggct
    14341 ggtggagatg acgcgtaaac gcacccgcga aagcattgag cacgtactgt gtaacgaatg
    14401 cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa acggtatgct atgaaatcat
    14461 gcgcgagatt gttcgtgtcc accatgctta cgactccgac cgtttcctgg tctatgcatc
    14521 tccggcagta gctgaagcct tgaaaggcga agagtcacac tcgctggcgg aagtggaaat
    14581 tttcgttggc aaacaggtta aagtacaaat tgaaccgctc tataaccagg agcagtttga
    14641 cgtcgtaatg atgtaaacag atgctgggcc gccatccggc aaagggtttt tgagtcacat
    14701 ttttagcaga caaggagtga cgggtgaggc gattgccggg gattttactg cttactggag
    14761 ccgcgctcgt tgtgatcgct gctctgctgg ttagcggcct gcgtattgct ttaccgcatc
    14821 ttgacgcctg gcgtccggaa atcctcaaca aaatagaatc cgcgactggc atgccggtag
    14881 aagccagtca gctctcagcc agctggcaga attttggccc gacgcttgaa gcacacgaca
    14941 tccgtgcaga actaaaagat ggcggcgaat tttcggttaa acgcgttact ctggcgctgg
    15001 atgtctggca gagcctgtta catatgcgct ggcagtttcg cgacctcact ttctggcagc
    15061 tgcgctttcg caccaacact cctatcacca gcggtggtag cgatgacagt ctggaagcca
    15121 gtcacatcag cgatctgttt cttcgtcaat ttgaccattt cgatctccgc gacagtgaag
    15181 tcagttttct gacgccatcc ggtcagcgcg ccgagctggc gatcccacaa ctcacctggc
    15241 tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag cctctccagc cttaccggac
    15301 agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga tgaggggttg ttaagcaatg
    15361 gtcgcgtctg gctacaggcg gatgacatcg acctgaagcc gtggctcggt aaatggatgc
    15421 aggacaatat tgctctggaa acggcacagt tctcccttga aggctggatg acgatcgaca
    15481 aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg cggtgccagc tggttgggcg
    15541 agaagcaaac gcatacgctg tcggtggata atctgaccgc gcatattacg cgtgaaaatc
    15601 cgggctggca gttctctatt cccgatacac ggatcacgat ggacggcaaa ccctggccga
    15661 gcggagcatt gacgctggcc tggataccgg aacaggacgt tggcggcaaa gacaataaac
    15721 gcagtgacga actccggatt cgcgccagta atctggagct ggcaggcctg gagggcatac
    15781 gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt ttggcgctcc acacaaccga
    15841 gcggcaagat taacactctg gcgctggata tcccgcttca ggcggcagac aagacccgtt
    15901 ttcaggcatc gtggagcgat ctggcctgga agcaatggaa attattaccg ggtgcggaac
    15961 acttctccgg gacgctttcc ggcagcgttg aaaatggttt gcttaccgcg tcgatgaagc
    16021 aggcaaagat gccttacgaa acggtattcc gtgcgccact ggaaatcgcc gacggccagg
    16081 caactataag ctggctgaac aatgacaaag gtttccagct ggatgggcgt aatattgacg
    16141 ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta cctgcaacct gctaacgatg
    16201 aaccctggct gggtattctg gctggcatca gtaccgatga tggttcacaa gcctggcgct
    16261 atttcccgga aaacttgatg ggtaaagacc tggttgatta cttaagtggc gcgattcagg
    16321 gcggtgaagc ggataacgcg acgctggttt atggtggcaa tccgcaactc ttcccctata
    16381 aacacaacga aggtcagttt gaagtgctgg tgccgctgcg caacgcgaag tttgccttcc
    16441 agccggactg gcctgcatta actaaccttg atattgaact ggactttatt aacgacggtt
    16501 tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg cgcgagtaat ctcaccgcag
    16561 tgatccctga ctactcgaaa gaaaaactgc tgattgacgc tgacattaaa ggtccgggta
    16621 aagccgttgg cccttacttt gatgagacac cgctgaaaga ttctctgggt gcgaccctgc
    16681 aagaactcca gctcgacggc gatgtgaatg ctcgcttaca tcttgatatc ccgctgaacg
    16741 gcgaactggt aaccgcgaaa ggtgaagtga cgctgcgtaa taacagtctg tttatcaaac
    16801 cactcgacag caccctgaaa aatttgagcg gtaaattcag ctttatcaat ggcgatctgc
    16861 aaagtgaacc actgacagca agctggttta atcagccgtt gaacgtggat ttttccacca
    16921 aagaaggggc aaaagcctac caggttgcgg tgaatctcaa cggtaactgg caaccggcga
    16981 aaaccagcgt tctgcctgaa gcggtgaacg aagcattgag tggcagcgtg gcgtgggatg
    17041 gtaaagtggg cattgatctg ccttatcatg ctggcgcgac ctataacgta gagctgaacg
    17101 gcgatctgaa taatgtgagt agtcacttac cttcaccgtt agccaaacct gcgggtgaac
    17161 cactagcggt aaacgttaag gttgatggca atctcaacag ctttgaatta accggacagg
    17221 ctggtgcgga taaccatttc aatagccgct ggttgcttgg tcaaaagctg acgctcgatc
    17281 gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt gccggaacaa agtggcgttg
    17341 aactcaatat gccgccgatg aatggtgccg agtggctggc cctgttccag aaaggcgctg
    17401 cggagagtgt cggtggtgca gcgagtttcc cacaacacat aacgttacgt acgcctatgt
    17461 tgtcactggg aaatcagcaa tggaataacc tgagtattgt ttcgcaaccg acggcaaatg
    17521 gcaccctggt tgaggcgcaa gggcgtgaaa tcaacgccac gctagcgatg cgtaataacg
    17581 cgccgtggct ggcgaatatc aaatatcttt attacaaccc gagcgtggcg aaaactcgtg
    17641 gtgattcaac gccgtcatca cctttcccga caacggagcg cattaacttc cgtggctggc
    17701 cggacgcaca aatacgatgc acagagtgct ggttctgggg gcaaaaattc ggtcgcattg
    17761 acagtgatat caccatttct ggcgatacgt taacgctgac caatggactg attgatactg
    17821 gtttctcgcg gcttactgcc gatggtgaat gggttaataa tccggagaat gaacgtacct
    17881 cgctgaaagg aaaactgcgc gggcagaaaa ttgatgccgc cgcagaattt tttggtgtca
    17941 cgacgcccat acgccagtcg tcatttaatg tggattacga tttacactgg cgcaaagcac
    18001 cctggcaacc agatgaggcg acgttgaatg gcatcattca tactcaactg ggtaaaggcg
    18061 aaattaccga aatcaatacc ggacatgccg ggcaattgct gcgcttattg agcgttgatg
    18121 ccctgatgcg taagctgcgt tttgatttca gagacacttt tggcgaaggg ttctattttg
    18181 actccattcg cagcaccgcg tggattaaag acggggttat gcacaccgac gacacgctgg
    18241 tggatggcct ggaggcggat atcgccatga aagggtcggt aaatctggta cgtcgcgacc
    18301 tgaatatgga agcggttgtc gcaccagaga tttctgcgac ggtgggcgtg gctgcggctt
    18361 ttgcggttaa ccccattgtt ggcgcggcag tatttgcagc cagtaaagtg ctggggccgc
    18421 tgtggagcaa agtctccatt ttgcgctatc acatttcggg tccgctggac gatccgcaaa
    18481 tcaacgaagt gttgcgccaa ccgcgtaaag aaaaagcgca atgatttgac gagggcgcgt
    18541 aattgcccca atctcatatg ataatcgttg ccaaaggcca acgagccgga acataactgt
    18601 aggccggata gggcgttcac gccgcatccg gcagccgtaa aaaatcctct actgcagtaa
    18661 ctaacgagta gcaaaaacga tgagtctgaa cctggtaagt gaacaattgc tagcggcgaa
    18721 cggcctgaaa catcaggact tgttcgcgat cctcggtcaa ctggccgaac gtcgccttga
    18781 ttatggcgat ctctattttc agtcgagcta tcacgaatcc tgggttttag aagaccgcat
    18841 tattaaagat ggttcttaca acatcgatca gggcgttggt gtgcgtgcaa tcagtggtga
    18901 aaaaaccgga tttgcttacg ctgaccaaat cagcctgctg gcgctggaac agagtgcgca
    18961 agcggcgcgc accatcgtcc gtgatagtgg tgatggcaaa gtacagacgc tgggcgcggt
    19021 agagcatagc ccgttgtata cctcggtaga tccgctgcaa agcatgagcc gtgaagagaa
    19081 gctggatatc ctgcgtcgcg tcgataaggt tgcccgcgaa gcggacaagc gcgtacaaga
    19141 agtgactgcc agcctcagcg gcgtttatga attaattctg gttgcggcca ccgacggcac
    19201 gctggcggcg gatgttcgtc cgctggtgcg tctttccgtg agcgttctgg tcgaagaaga
    19261 tggcaaacgc gaacgcggtg ccagtggcgg cggcggtcgt tttggttatg agttcttcct
    19321 tgccgatctt gacggcgaag tccgtgcgga tgcatgggca aaagaagcag tgcgtatggc
    19381 gctggtcaat ctttctgccg ttgctgcacc agcgggcacc atgccggtag tacttggcgc
    19441 aggttggccg ggcgtgctgt tgcatgaagc ggtaggtcac ggtctggaag gcgacttcaa
    19501 ctgccgcggc acttcagtat ttagtgggca ggtcggggag ctggtggctt cagaactgtg
    19561 taccgtggtt gatgacggca cgatggtcga tcgccgtggt tcggtggcga ttgatgacga
    19621 aggtacgcca ggccagtaca acgtgctgat tgagaacggc attctgaaag gctacatgca
    19681 ggataaactc aacgcgcgtt tgatggggat gacgccgact ggcaacggtc gccgtgaatc
    19741 ctacgcccat ctgcccatgc cgcgtatgac caacacctat atgctgccgg gtaaatcgac
    19801 cccgcaggaa attattgaat ccgttgagta cggtatctat gcaccgaact ttggtggcgg
    19861 tcaggtggat attacatccg gcaaattcgt tttctccact tcagaagcgt atctgattga
    19921 aaacggtaaa gtaacgaagc cggtgaaagg cgcgacgttg attggttccg gtatcgaaac
    19981 catgcagcag atttcgatgg ttggcaacga cctgaaactg gataacggcg tgggtgtctg
    20041 cggtaaagaa gggcaaagtt tgccggttgg cgtgggccag ccaacgctga aagttgataa
    20101 cctgactgtt ggcggtactg cgtaataatt tcattatttt cagaaggata attaatcttt
    20161 ctacgtgcac gaacggtccc ctcgctcctc tggggttagg gtgaggggaa cccgttggca
    20221 caggtttgta gaacgtaaca gtacaatatg aattacttct ctttcccgcg cccgtgcatc
    20281 tcctgaaaca atttaccgac ctcaacaaaa taatccgtca gcgagttgat cacgacctgt
    20341 accttcagcg gcagcttatc tttttcggta tataacgcat aaaccgggcg tggatctgac
    20401 tgataacgcg gcagcaggat ctccagctca ccgcgattga tctcgttgat cacccacatc
    20461 agcggcacgt aggcgatccc ggcacccgcc gttagccagc gcaccagcgt catcggatca
    20521 ttagtcacaa atctcccttg cgggatcagg cgagtcgaga tcccttccgg tgcaatcagt
    20581 tcaaattcat tgtcgggccg cacgctgtat tcaagccatg aatggctact taaatcggcg
    20641 ggtttttccg gtatgccgta ttgtgtgaga tagcttttcg cggcgcacac caccattggc
    20701 atcgcgccaa gacggcggga aaacaggctg gaatcctgca acgcgccgac gcggatcacc
    20761 acatccagac cgtcggcaat caggtcgggg gctggaattc cggtaaccag attgacgctc
    20821 aaacctgggt attctttcag cattttggct gtcagcccgg cgagaacatt ttgtgccata
    20881 gttgaagaac agccaatgcg tagcgtcccg atgggggtgt tattgaaggc atacagttgc
    20941 tcatgaacat cctgcacttc atgaagcata cgacggcagc cctggtagta aattctaccg
    21001 gcttcggtca ggccaatgct gcgtgtgcta cggtttaaca gctttacctg caactcatct
    21061 tccagttttg acaccgtctg actgatggac gaaacgctca tctgtagctg tctggcggcg
    21121 gcggtaaaag agccaaattc aactactttg gcaaacaccg acatgcgttt tagtcgttcc
    21181 attattcact ctgacttaaa agtgatttag atcacataat atagataaca gcataacagt
    21241 tacgctaata tattaaatat caatctacag caatgttgct ctcgcccggc tttccatgcc
    21301 cttctctgcg gcgacagatg ctgaaaataa taacgcctgc tctctctata caaccaaggt
    21361 caacatgagt ctgtttcccg ttatcgtggt gtttgggctg tccttcccac cgatattttt
    21421 tgaattgctt ttatcactgg cgattttctg gctggtgcgc cgggtacttg tgccaacagg
    21481 tatctacgac tttgtctggc atccggcgtt gttcaacacc gcgctctatt gctgcttgtt
    21541 ttatttgata tcgcgactgt tcgtttgagg ttgaagtgaa aacactaata agaaaattct
    21601 cccgtacggc catcacggtc gtattagtca ttctggcctt catcgcaatt tttaatgcct
    21661 gggtctatta caccgaatcc ccctggacgc gtgacgcgcg ctttagcgct gacgtcgttg
    21721 cgatcgcgcc ggacgtttct ggactcatta cccaggtgaa tgttcatgat aaccagctgg
    21781 tgaaaaaagg acagatactg ttcaccatcg accagccgcg ctatcaaaag gcgcttgagg
    21841 aagcgcaagc cgatgttgcc tattatcagg tactggcaca ggagaaacgc caggaggccg
    21901 gacgtcgtaa ccgtctcggt gtgcaggcga tgtctcgcga agagatcgac caggctaaca
    21961 acgtactaca aacggttctg catcagttag cgaaagcgca ggcgacccgc gatctggcaa
    22021 aactggatct tgaacgcacg gtgatccgcg cgccagcaga tggctgggtg accaacctca
    22081 acgtctatac cggtgagttt attactcgag gatcaacggc ggttgcgctg gtgaaacaga
    22141 actccttcta tgtactggcc tatatggaag aaactaagct ggaaggggtg cgtccggggt
    22201 atcgtgcaga gatcacgccg cttggcagta acaaagtgct gaaagggact gttgatagtg
    22261 ttgccgctgg ggtcaccaac gccagcagca cgcgtgacga caaagggatg gcgaccatcg
    22321 actctaacct tgaatgggtg cgtttggcgc aacgtgttcc ggttcgtatt cgtctcgaca
    22381 accagcaaga gaacatctgg cctgcgggca ccactgctac agtggtggtc actggcaaac
    22441 aagatcgcga cgaaagccag gattcgttct tccgtaaaat ggcccatcgc ctgcgtgagt
    22501 ttggttaatc acgatgggta ttttctccat tgctaaccaa catattcgct ttgcggtaaa
    22561 actggcgacc gccattgtac tggcgctgtt tgttggcttt cacttccagc tggaaacgcc
    22621 acgctgggcg gtactgacag cggcgattgt tgccgccggt ccggcctttg ctgcgggagg
    22681 tgaaccgtat tctggcgcta ttcgctatcg tggctttttg cgcatcatcg gcacatttat
    22741 tggctgtatt gccggactgg tgatcatcat tgcgatgatc cgcgcaccat tattgatgat
    22801 tctggtgtgc tgtatctggg ccggtttttg tacctggata tcctcgctgg tacgaataga
    22861 aaactcgtac gcgtgggggc tggccggtta taccgcgctg atcattgtga tcaccattca
    22921 gccggaacca ttgcttacgc cgcagtttgc cgtcgaacgt tgtagcgaga tcgttatcgg
    22981 tattgtgtgt gcgattatgg cggatttgct cttttctccg cgatcgatca aacaagaagt
    23041 ggatcgagag ctggaaagtt tgctggtcgc gcaatatcaa ttaatgcaac tctgtatcaa
    23101 gcatggcgat ggtgaagttg tcgataaagc ctggggcgac ctggtgcgac gcaccacggc
    23161 gctacaaagc atgcgcagca acctgaatat ggaatcttcc cgctgggcgc gggccaatcg
    23221 acgtttaaaa gcgatcaata cgctatcgct gacgctgatt acccaatcct gcgaaactta
    23281 tcttattcag aatacgcgcc cggaattgat cactgatact ttccgcgaat tttttgacac
    23341 gccggtagaa accgcgcagg acgtccacaa gcagctcaaa cgcctgcgga gagttatcgc
    23401 ctggaccggg gaacgggaaa cgcctgtcac catttatagc tgggtcgcgg cggcaacgcg
    23461 ttatcagctt ctcaagcgcg gcgttatcag taacacaaaa atcaacgcca ccgaagaaga
    23521 gatcctgcaa ggcgaaccgg aagtcaaagt agagtcagcc gaacgtcatc atgcgatggt
    23581 taacttctgg cgaaccacac tttcctgcat tctgggcacg cttttctggc tgtggacggg
    23641 ctggacttcc ggcagtggtg caatggtgat gattgcggtc gtgacgtcac tggcaatgcg
    23701 tttgccgaat ccacgcatgg tggcgatcga ctttatctac gggacgctgg ccgcgctgcc
    23761 gttagggctg ctctactttt tggtgattat ccctaatacc caacagagca tgttgctgct
    23821 gtgtattagc ctggcagtgc tgggattttt cctcggtata gaagtacaga aacggcgact
    23881 gggctcgatg ggggcgctgg ccagcaccat aaatattatc gtgctggata acccgatgac
    23941 tttccatttc agtcagtttc tcgacagcgc attagggcaa atcgtcggct gtgtgctcgc
    24001 gttcaccgtt attttgcttg tgcgggataa atcgcgcgac aggaccggac gtgtactgct
    24061 taatcagttt gtttctgccg ctgtttccgc gatgactacc aatgtggcac gtcgtaaaga
    24121 gaaccacctc ccggcacttt atcagcagct gtttttgctg atgaataagt tcccagggga
    24181 tttgccgaaa tttcgcctgg cgctgacgat gattatcgcg caccagcgcc tgcgtgatgc
    24241 gccgatcccg gttaacgagg atttatcggc gtttcaccga caaatgcgcc gcacagcaga
    24301 ccatgtgata tctgcccgta gcgatgataa acgtcgtcgg tactttggcc agttgctgga
    24361 agaactggaa atctaccagg aaaagctacg catctggcaa gcgccaccgc aggtgacgga
    24421 accggttcat cggctggcgg ggatgctcca taagtatcaa catgcgttga ccgatagtta
    24481 agtcaaaacc gacgccaaaa gcgtcggttt tttcatggct atacttagcg atgcacggca
    24541 gaactcgccg cgaaacgtga cggtggcaac agatgaatat ttataccttt gattttgatg
    24601 agattgagag tcaggaggat ttttatcgtg actttagcca aacctttggt ctggctaaag
    24661 ataaggtacg cgatctcgac tcactatggg atgtgttaat gaacgatgtc ctgccgctac
    24721 cacttgagat tgaatttgtt catctgggag agaaaacgcg tcgccgtttt ggcgcgttaa
    24781 tattgctgtt tgatgaggca gaggaagagc tggaagggca tttgcgtttt aatgttcgtc
    24841 attagcgcaa aaaaaagccc ccgaaccggg ggcaatatcg tcggacaaga cgatgagggt
    24901 ttatttgtac agttcagccg tagcgtgcca ggtgtcaccg ctacgagctt cagtaatctg
    24961 gtaggccgtt gcgcctttct cttccgcttt tttgttcagc atttcacgca tatccattgg
    25021 cgaagacgcc acaccactta cggatacggt cccgattgct tcacgatttt gtgcttgtgc
    25081 agcatcaatg gagtcggcag cgaatgcacc gaaagagaga acagaaagta cgcttaatgc
    25141 agctacagtg gttttgattt tcatgatttt tacctcgaca taatctttta gctgggcctt
    25201 tgtttcgtga cccatatcac aaaatcaagt atacactaat tattgaac
//
LOCUS       DAZFRJ010000058        23868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000058 DAZFRJ010000000
VERSION     DAZFRJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.058"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..1642
                     /locus_tag="ACHKUN_002103"
     CDS             62..1642
                     /locus_tag="ACHKUN_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO6660355.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            1642..2850
                     /locus_tag="ACHKUN_002104"
     CDS             1642..2850
                     /locus_tag="ACHKUN_002104"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO6660356.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            2819..3415
                     /locus_tag="ACHKUN_002105"
     CDS             2819..3415
                     /locus_tag="ACHKUN_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO6660357.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3487..4434
                     /locus_tag="ACHKUN_002106"
     CDS             3487..4434
                     /locus_tag="ACHKUN_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6660358.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(4514..4590)
                     /locus_tag="ACHKUN_002107"
     tRNA            complement(4514..4590)
                     /locus_tag="ACHKUN_002107"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4554..4556),aa:Met,seq:cat)
     gene            4799..5896
                     /gene="mltA"
                     /locus_tag="ACHKUN_002108"
     CDS             4799..5896
                     /gene="mltA"
                     /locus_tag="ACHKUN_002108"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO6660359.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            5973..6779
                     /gene="tcdA"
                     /locus_tag="ACHKUN_002109"
     CDS             5973..6779
                     /gene="tcdA"
                     /locus_tag="ACHKUN_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO6660360.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(6830..7273)
                     /gene="csdE"
                     /locus_tag="ACHKUN_002110"
     CDS             complement(6830..7273)
                     /gene="csdE"
                     /locus_tag="ACHKUN_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO6660361.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAIEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(7273..8478)
                     /gene="csdA"
                     /locus_tag="ACHKUN_002111"
     CDS             complement(7273..8478)
                     /gene="csdA"
                     /locus_tag="ACHKUN_002111"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO6660362.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            8670..8897
                     /gene="ygdI"
                     /locus_tag="ACHKUN_002112"
     CDS             8670..8897
                     /gene="ygdI"
                     /locus_tag="ACHKUN_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO6660363.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9248..10165
                     /gene="gcvA"
                     /locus_tag="ACHKUN_002113"
     CDS             9248..10165
                     /gene="gcvA"
                     /locus_tag="ACHKUN_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO6660364.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            10184..10579
                     /gene="ygdD"
                     /locus_tag="ACHKUN_002114"
     CDS             10184..10579
                     /gene="ygdD"
                     /locus_tag="ACHKUN_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO6660365.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            10572..11672
                     /gene="rlmM"
                     /locus_tag="ACHKUN_002115"
     CDS             10572..11672
                     /gene="rlmM"
                     /locus_tag="ACHKUN_002115"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO6660366.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(11716..12447)
                     /gene="fucR"
                     /locus_tag="ACHKUN_002116"
     CDS             complement(11716..12447)
                     /gene="fucR"
                     /locus_tag="ACHKUN_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO6660367.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12505..12927)
                     /gene="fucU"
                     /locus_tag="ACHKUN_002117"
     CDS             complement(12505..12927)
                     /gene="fucU"
                     /locus_tag="ACHKUN_002117"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO6660368.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(12929..14347)
                     /gene="fucK"
                     /locus_tag="ACHKUN_002118"
     CDS             complement(12929..14347)
                     /gene="fucK"
                     /locus_tag="ACHKUN_002118"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO6660369.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(14456..16231)
                     /gene="fucI"
                     /locus_tag="ACHKUN_002119"
     CDS             complement(14456..16231)
                     /gene="fucI"
                     /locus_tag="ACHKUN_002119"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO6660370.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(16264..17580)
                     /gene="fucP"
                     /locus_tag="ACHKUN_002120"
     CDS             complement(16264..17580)
                     /gene="fucP"
                     /locus_tag="ACHKUN_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO6660371.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            18127..18774
                     /gene="fucA"
                     /locus_tag="ACHKUN_002121"
     CDS             18127..18774
                     /gene="fucA"
                     /locus_tag="ACHKUN_002121"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6660372.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            18802..19950
                     /gene="fucO"
                     /locus_tag="ACHKUN_002122"
     CDS             18802..19950
                     /gene="fucO"
                     /locus_tag="ACHKUN_002122"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO6660373.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(20005..20760)
                     /gene="xni"
                     /locus_tag="ACHKUN_002123"
     CDS             complement(20005..20760)
                     /gene="xni"
                     /locus_tag="ACHKUN_002123"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO6660374.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(20872..22239)
                     /gene="sdaB"
                     /locus_tag="ACHKUN_002124"
     CDS             complement(20872..22239)
                     /gene="sdaB"
                     /locus_tag="ACHKUN_002124"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO6660375.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(22297..23586)
                     /gene="sdaC"
                     /locus_tag="ACHKUN_002125"
     CDS             complement(22297..23586)
                     /gene="sdaC"
                     /locus_tag="ACHKUN_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO6660376.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         5995 a         5626 c         6165 g         6082 t
ORIGIN      
        1 tctattcgca tcaaagtatt aatggactaa ttatacataa cattttcatg agataaccag
       61 tatgaaacaa aagaaagcct ggagtttttt tcagagcctg gggaaggcat ttatgtatcc
      121 catcgctctg ttaagtgtat gtggtatgat gttagggctg ggaagtggtt tagccagcga
      181 tgatatggga aaattcattc cagtgttggc tattccgtta attaaaacca tacttgattt
      241 tattgttagc cttggtttgt ttgccttcgt taatttacct gtattgtttg cgatagctat
      301 acctttggga ttattaaaag agaaagatga taaagcctat ggcgcttttt ctggcttaat
      361 tggctttatg gcgatgcatc ttggaacgaa cttttatctt aaacagcacg acttattggt
      421 agctgctgac caaatgtcga cacatgggca aaccatcata ctcgggatcc agtcctacaa
      481 taccagcgtg ttgggtggga ttgtggcagg gttattagtc gccagcatgt ataaaaagat
      541 cgttaactta cgtattcctg aatcattagg gttttatagt ggcccacgcc ttgtgcctat
      601 cattacactg attgtgatga gtggatttgg tctgttcatt ccttttatct ggccgccatt
      661 tttcaatctt ttcatgctca ttggtcactg gatttcaact tccggtcctg ttggttattt
      721 cttctatgct gttgccgaac gcgtgacgat accttttggc ttaaatcact tggtgacatc
      781 agttttccgc tttaccccaa tcggcggatc ggcagtgatt ggcggtgaag agtattacgg
      841 caccctgaac atgtttatgg catacgtcaa agagaatgcg gtcattccgc tggatttggc
      901 ggggaaaatg gagcagggca aactgatgat tcagtatggt ctggctggag ccgcgctggc
      961 gatgtatcgc actgctcatg ctcaaaacag aagggctatc aaagcattgc tgatttccgg
     1021 tgtgctcacg gtaattattg gcggcgtcag cgagccgatt gaatttctgt tcttatttgt
     1081 cagcccgctg ttgtttgtct tccatgcctt tatgaatgga ttggctaaca tggttctgcc
     1141 atatatgggc gtgaagatgg gctttactgg cgatctgatt caatttatta gctttggtgt
     1201 cttacgtggc acaagaacgg gatggccatt agcggtatgt gttgaagtgg cttatttctt
     1261 catttattac tttgtgttcc gttggaccat tctcaaattt aacctgatga ctgtaggccg
     1321 tgaagagtcc agtcctgtca cgctgaacgc tcacgaagaa actgcaatag ctaacatccc
     1381 agttcatgat aaatcagagt tgcaggcagc ggagcaaatg gttaaggctc tgggtggtaa
     1441 agaaaatatt aaatcactgg ataattgtgt aactcgtttg cgtttaacta tcgcggatat
     1501 gggattgata gatgaggctg caataaaaag ggccggagga attgcggttg tgaaacttga
     1561 tcaaaatacc ttacaagtca ttatcggtac aaaagtcatc gccctacgtc gggatatgga
     1621 taattacatg gggatatgtt gatggatatt tttaatacct caatttctcg taaaggaaca
     1681 tattgcacac aatgggactt ttgtgaagac agatttggtg taaaagatgt attaccattc
     1741 tctatttcag atatggatct tccgataccg gaagctatca tcagaacatt aaaaaaacgt
     1801 ctggaacacc cgattttagg gtacagtcgt tggcagcatg atgactatct gaattcgata
     1861 gtcaattggt atcatcaaca atataaaaca gagattcaac cagaatggat cacctatagc
     1921 ccaagcgtga tgtattcaat atctaaggca atagaaatat tgacattgaa tggagataat
     1981 attctcattt tcacgcctgt ttataacgca ttttttgatg tgattaagca taatggtcga
     2041 aacatgctaa caacctcttt gatgaaagaa aatgggagag gctacgttat caactggcaa
     2101 gactttgagc ttaaaattaa aagcgcgaag atggtgttat tatgtaatcc tcataacccg
     2161 acaggaacgg tctggtcgga agacgatctt cacagaatcg cagcatgctg tgcaaggcat
     2221 aatatttggt tatgttcaga tgaaatacat agtgattttg tttttaatca tagtttcacg
     2281 tcagcgctaa caataaaaga tgacaaagtg gtggtgttta attcgatttc gaaaacattt
     2341 aatgttcctg cgctaaccgg atcatatatg atttcaactg acgataattt taatcataag
     2401 ttcagaacga tatcgaggta tcgcgatttc gtaaactccc catcagtact gaatgtaatt
     2461 gcgaccattg ttgcctataa tgaatgtgaa gaatggttaa agtcgttaaa ggtacatatt
     2521 gcctctaaca ttcaatttac cttgcagtat ttaaatgaaa atattccgga attgatcgta
     2581 tggccagctg agggctgcta tttttcgtgg gttgattgct cggcccttgg ttatcccttt
     2641 gataaatttt accgtcgatt aattaacgaa gggagagtag gtattatggc ggggcatgtt
     2701 tatggtactg aaggagaggg ctatctgcgt cttaatgtgg cctgcagtcg agaaaaatta
     2761 tatatggggc ttgcgcgcct tgcgagtgta attaaaaata ttaatcagga aaaagaaaat
     2821 gaacaatacg gatcttatcc atcttattaa acgttttatg cgtaacgaac ataaagcagt
     2881 agaagaggtt attgattccc ccctttcgga agtcgcgaat ttaattaaaa tattacaatc
     2941 ttgccaggga aaagttgttt ttattggtgt tgggaaatct ggcattattg ccagaaaact
     3001 tgctgcaaca tttgccagca ccggaactcc ctcgtttttt gttcacggta cggaagcggt
     3061 acacggcgac cttgggatgg tggcgaaaga cgatgttgtt attcttattt caaacagtgg
     3121 tgagacggcg gaaatcctgg cgacactgcc tagtttgaaa aaaatgggta attatttgat
     3181 ctcttttacc cgcagtcatc actcatcgct tgcgataagt tgtgatttat ctgttgaaat
     3241 ccccgtcaaa agtgaggcgg ataatttagg tttagcaccg tcatgctcgt ctactgttgt
     3301 tctggttgtt ggtgatgcgg tggcgcttgc actctctgaa ttgaaaaaat ttactcgtgc
     3361 tgatttcggc ttatatcatc caggaggtgc actcggcatt aaagcaaatt cataatttca
     3421 aagtataaat acagtaaccg gtcacgccat cttttggggt ggcgttcatt gaagtgagtg
     3481 tcagctatga aaaatgttct ggtcaccgtc cccagctttt cagcacgctg tgtttccgcc
     3541 agcaagttgc taagagagaa taattttaat cttattatta agaataatgt ggagcatctg
     3601 cttaaatcag aatccactgc attacgagaa tctatttgcg cggtcatcgc cggaaaagat
     3661 tgttatcagg cggacacgct atcattgctg cctggtgtga aaataatatc gagattcggg
     3721 acggggatag ataatattga tcttcgtgca gcacaacaat ccggtattgt agtcaataat
     3781 gcggttggga ttaattcaaa tgctgtcgcg gaatttatca ttggacttat cttcgcgagc
     3841 atgagaaata ttccaggcag ctatcatgcg atgcaaaatg gctactgggg tgagtcgcat
     3901 ggctatgaat tacaaggcaa acggattggc atggttggct acggcaatat aggtaaaacc
     3961 ctggcaaaaa ggttatccgg ttttgatgtt gagcttttag cttatgacaa acaacccgat
     4021 tatcaggttg ctgacaaagt tggagttcag tttgtatcaa ttgaagatat ctttatgcag
     4081 tcgcatgtca tcattgttct tttgcctttt tctcctgagc tggagaactt cattagtcat
     4141 aaatatttgt cgatgatgcg caatggtgct ctaatcatca atgccgcaag gggaaagttg
     4201 ctggatgagg gcgctttact acaggtgatt gaagaacgaa atgtgtttgc ggcgctggat
     4261 gtgtttagct ctgaaccgtt ggcgcaattt agcccactgc tacatgccaa aaatatcatt
     4321 acaacgccgc atattgcggc cgccaccgtt gagtcatacc agcaaaccgg tacacacgtt
     4381 gcccagtcga taattgatta cttcgcagga agggagataa aaaacgtgtt gtgagttcag
     4441 cgaacgaagt ttttaaatac aaaaaaaacc gcaatttgtg gaacacattg cggtttatca
     4501 gcattctaag tattggttgc gggggccgga tttgaaccga cgaccttcgg gttatgagcc
     4561 cgacgagcta ccaggctgct ccaccccgcg tccgtggatg cgcactatac tccgctcgcg
     4621 ttttcatgca accttttttt taccccgatc acggatttag catgattttg aacaaaaaca
     4681 aaccactaaa gcctattttt tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt
     4741 aaacgcggcg gtttgttatc ttcgttgcgc cttatttttt aacctgaaga agagaacaat
     4801 gaaaggacgt tgggtaaagt accttcttat gggcacggtt gtggcaatgc ttgccgcctg
     4861 ctcttccaaa ccaaccgatc gcggacagca atataaagac gggaaattta cccagccttt
     4921 ctctctggtg aaccagccag atgccgttgg cgcgccgatt aacgccggtg attttgccga
     4981 gcaaattaac catatccgta attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta
     5041 taacgcggtg caagagtggc tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg
     5101 cattgatgcc tggcagatgg aaggtgccga caactatggt aacgtgcagt ttacaggcta
     5161 ttacacgccg gtaattcagg cgcgccatac ccgccagggc gagttccagt atcctattta
     5221 ccgtatgccg ccaaaacgtg gtcgtctgcc gtctcgtgcg gagatctacg caggggcgct
     5281 gagtgataaa tatattctcg cttacagcaa ctccctgatg gataacttca ttatggatgt
     5341 gcagggtagc gggtatatcg actttggtga tggcagtccg cttaactttt tcagctatgc
     5401 agggaaaaac ggtcatgcct atcgcagcat tggtaaggtg ctgatcgacc gtggcgaagt
     5461 gaaaaaagaa gatatgtcga tgcaggcgat tcgtcattgg ggcgaaacac acagtgaagc
     5521 cgaggttcgc gagctgctgg aacagaaccc gtctttcgtt ttctttaaac cgcaatcttt
     5581 tgcaccggtg aaaggggcaa gtgcggtgcc gctggttggc cgtgcttctg ttgcctccga
     5641 tcgttcaatt attccgccag gtactacctt gctggcagaa gtgccgctgc tggataataa
     5701 cggcaaattt aacggtcagt acgaattgcg tctgatggtg gcgctggatg tcggtggcgc
     5761 aatcaaaggc caacacttcg atatctatca agggatcggg ccggaagccg gacatcgcgc
     5821 aggttggtac aaccactatg gacgtgtctg ggtgctgaaa accgccccgg gcgcaggtaa
     5881 cgtctttagc ggctgatgtg ttattctgta taccgttttt acggatacga cagggtgagg
     5941 gataacctca ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt gatgcatggc
     6001 gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcgttgcaa ctgtttgctg
     6061 acgcgcatat ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg gaagcgctgg
     6121 cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc gtcaccaata
     6181 ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg gaagtcatgg
     6241 cggagcgtat tcgccagatt aacccggagt gccgtgtaac ggtggtggat gatttcgtga
     6301 cgccggataa cgtagcgcag tatatgagcg tggggtattc gtacgtgatt gatgccattg
     6361 atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa atcccgctgg
     6421 tcacgaccgg tggcgcgggt gggcagattg acccgacgca gattcaggtt accgatctgg
     6481 cgaaaacgat tcaggatccg ctggcggcga agttgcgcga gcgcctgaaa agcgattttg
     6541 gcgtagtgaa aaacagtaaa ggtaagctcg gcgtcgattg cgtgttttct actgaagcgc
     6601 tggtgtaccc gcagtcagac ggtactgtgt gtgcgatgaa agccacggca gaagggccga
     6661 agcggatgga ttgtgcatct ggatttggcg cggcaacgat ggtgaccgcc acctttgggt
     6721 ttgttgcggt ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt cagggttaaa
     6781 caatcaggtc ggatgcgacg cgaacgtctt atccgacctg gttctgtgct taaacctgct
     6841 tcgtcgcagc gataatcgcc tcgcttaacg cattcaaccc ctggctgcgt gaggcgctaa
     6901 gctgcccacg caatcccagc tcatcaaaca atgccagcgg cgactgtgcc tgcaattcgg
     6961 cggcggtttt cccctcaatg gcagtcaaca gcaccgccag caggccgcgc actatacgtc
     7021 cttcgctgtc gccaaagaag tgcattttac cgttttcagc cacggtatat cccagccaga
     7081 cgcggttttc gcatccggca atctctttag cctgcgcttt taactcgtct ggcaaagccg
     7141 gaagctgttt ccccagcatg atcaactggc gatatttatc ttcccattgc gtcaacggtg
     7201 cgaaggtatt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt ccggcgaatt
     7261 gcgggttcgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat tcaccagcgc
     7321 atccacatca ctctttgtat tatatggcgc aaaagaggcg cgcagtgtgc cggttacgcc
     7381 taattctgcc agtagcggct gagcgcaatg ttgcccggcc cgcaaggcaa taccgtactc
     7441 cgccagcagc gtcaccatat cgctgtgatg aacgccagca aaatcaaagg ccagcaggct
     7501 ggaatcctgg cagcggaatg aacggaaacc tggacgtttc gccagcgcat cttccgccag
     7561 tgttgctaag ctacggctcc agttttcggc ctggttgata tcgtaatctg ccagccattc
     7621 cagcgccgcg cttaatccta tgacaccagc cacatttggc gttcctgctt ccagtttcca
     7681 cggcgcagat tgagtcgtga agccgtcaaa actcacttcg tgaaccattt tgccgccgcc
     7741 cagccagggc gacatcgctt ccagcagttc tgatttgcca tacagcacgc cgatacctgt
     7801 cgggccatac agtttgtgac ctgaaaaagc atagaaatca atattcagtt gctgaacatc
     7861 cgcggggaaa tgcaccgccc cctgagcacc atcaaccatc accaccatcc cggctgaatg
     7921 agcaaaggta atcgctcgcg ccagatccgg gcaaccgcca gtaacgttcg acatctgacc
     7981 caacgccaga atccgactac ggggagtaat cagttctggc aacaaatcga catccggcag
     8041 tcgctgcgca ttaagcggca atttcaccac tttggctcca gtttgttggg cgaccatcag
     8101 ccaggggacg aggttggcgt ggtgttctgc cacgctgaca ataatctcat cgcccagttg
     8161 cagacgcgga cgcgcatagc attgtgcaac catgttgatg gattcagtgg tgccgcgcgt
     8221 ccagacgata gttttatcat ccggtgcatt cagtaattgc gccactttct ctcgtgcagc
     8281 ttcataacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga cgtttccggc
     8341 gctcaggctg taaaactgtc gggtggcttc aaccacggct tcaggtttaa gcgcggtcgc
     8401 ggcgctgtcg agatagacgc ccgcatcctg tagagcggga aactgggcgc gaaactgcgc
     8461 gggattaaaa acgttcatgg tactcctcgg cttgatgcac cgatcgtgac gcaaaattca
     8521 ctttgataca aggagagtgc taaccatcag aattatctgg atatcctggc gaagccgcgt
     8581 aagtaacata tgctgaatag tgttaggcgt taattttagc ctgttaatca atactattaa
     8641 gatttattaa tcgctttaag gagaagatga tgaaaaagac tgccgcaatt atttctgcct
     8701 gtatgctgac ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg cacaccaatg
     8761 acggacgtac catcgtctct gacggcaaac cacagactga taacgatacc ggtatgattt
     8821 cgtataaaga cgctaatggc aacaaacagc agatcaaccg tactgacgtg aaagagatgg
     8881 tcgaactgga tcagtaattc acgatcgcaa ggtaaaaaaa agcaccgcaa ttaggcggtg
     8941 ctacattaat cactatggac agacagggta aatgtacagg aagtgaaaaa aggtagcttt
     9001 gctaccatgg tctgaatcgc agaccaattg caaacacaac aacacaacat cacaaccgta
     9061 agccaaaagt tcaccagaac acgcattccg ataaaacttt tcgttccggc tcaggaagtg
     9121 ccgccactat aggtatttgc tggtagaagc tcaacggaca atttataatg gctcagatta
     9181 aaaaaactaa taggttacac agtgtgatct aattgttaaa ttcatttaac atcaaagttt
     9241 aaaagccatg tctaaacgat taccaccgct aaatgcctta cgagtttttg atgccgcagc
     9301 acgccattta agtttcactc gcgcagcaga agagcttttt gtgacccagg ccgcagtaag
     9361 tcatcaaatc aagtctcttg aggatttttt ggggctaaaa ctgttccgcc gccgtaatcg
     9421 ttcactcctg ctgaccgagg aagggcaaag ctatttcctc gatatcaaag agatattttc
     9481 gcaattaacc gaagcgacgc gtaaactcca ggcccgtagc gccaaggggg cgttgacggt
     9541 cagtttactc cccagtttcg ccattcattg gttggttccg cgactttcca gctttaattc
     9601 agcttatccg ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag ataagctggc
     9661 ggatgatgtt gatgtggcga tattttatgg tcggggcaac tggccggggc tacgggtgga
     9721 aaaactgtac gccgaatatt tattgccggt gtgttcgccg ctactgctga caggcgaaaa
     9781 acccttgaag accccggaag atctggctaa acatacgtta ttacatgatg cgtcacgccg
     9841 tgactggcag acatataccc gacagttggg gttaaatcat atcaacgttc agcaagggcc
     9901 aatttttagt catagcgcca tggtgctgca agcggctatt cacgggcagg gagtggcgct
     9961 ggcaaataac gtgatggcgc aatctgaaat tgaagccgga cgtcttgttt gcccgtttaa
    10021 tgatgttctg gtcagtaaaa acgcttttta tctggtttgt catgacagcc aggcagaact
    10081 gggtaaaata gccgcctttc gccaatggat cctggcgaaa gccgctgctg aacaagaaaa
    10141 attccgcttt cgttatgaac aataatttac gtagggtacg accatgacca gccgttttat
    10201 gctgattttc gccgccatta gcggcttcat ttttgtggct ctgggcgctt ttggcgcgca
    10261 tgtgttaagt aaaaccatgg gggccgttga gatgggctgg atccagaccg gcctcgaata
    10321 ccaggcgttt catacgctgg cgatcttagg tctggcggtg gcaatgcagc gtcgcatcag
    10381 tatctggttt tactggagta gcgttttcct cgcgttaggc acggtgctgt tcagcggcag
    10441 cctttattgc ctggcactgt cccatctgcg tttgtgggcg tttgtcactc cggttggcgg
    10501 cgtgagcttc cttgcgggct gggcgttaat gttagttggt gctatccgtt taaagcgcaa
    10561 gggcgtaagt catgaataag gttgtattgc tgtgccgtcc gggctttgaa aaagagtgcg
    10621 ccgcagaaat taccgataaa gccggccagc gggaaatttt cggttttgcc cgcgtgaaag
    10681 agaatgcggg ttatgtcatt tatgaatgtt atcaacctga tgatggcgat aagttaatcc
    10741 gtgagctgcc gttcagttca ttaatttttg cccgccagtg gtttgtggtg ggggaactcc
    10801 tgcagcattt gccgccagaa gatcgtatta cccccattgt cggcatgtta cagggcgtag
    10861 tagagaaggg cggtgaactg cgtgttgaag ttgccgatac caacgaaagc aaagagttgc
    10921 tgaaattctg ccgtaaattc accgtgccgc tacgcgctgc cttgcgcgat gccggggtgc
    10981 tggcgaacta tgaaacgccg aagcgtccgg ttgtgcatgt attcttcatt gcaccaggtt
    11041 gctgctatac cggttactca tacagcaaca ataattcgcc gttctatatg ggcattccgc
    11101 gcctgaaatt tccggcagat gcgccgagtc gttccacact caaactggaa gaggcatttc
    11161 atgtgtttat tcctgcggat gagtgggatg aacgcctggc gaacgggatg tgggcggtgg
    11221 atttgggcgc ttgcccaggc ggctggacct accaactggt gaagcgcaac atgtgggttt
    11281 attccgtcga caacggcccg atggcgcaaa gtctgatgga taccgggcag gtgacgtggc
    11341 tgcgggaaga cggtttcaaa ttccgtccga cacgcagcaa tatctcctgg atggtatgcg
    11401 atatggttga aaaaccggct aaagttgcgg cattgatggc gcagtggctg gttaatggct
    11461 ggtgccgtga aaccattttc aatctcaaac tgccgatgaa aaaacgctac gaagaagtat
    11521 cacacaatct ggcgtatatt caggcacagc ttgatgaaca tggcataaat gctcagattc
    11581 aggcgcggca gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc cgcatctggg
    11641 ctgcggtggg tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt cgtctccggc
    11701 ctgctaccct ttccgtcagg ctgttaccaa agaagttgca acctggcgct catcagaaat
    11761 aatgtgcgtt acctcatcca gatgcccgat gcgggcttcc cctgaacgat taaatttact
    11821 tttatcaatc aataacaacg attgcgcggc acgttttaat agcatcgatt tgtaatcagc
    11881 gttgatggca ttggagtccc acagtgcgcc gctgctatcg atcccttcac aagaaaaaat
    11941 aaacagatcg atttccagcg atttaagttg ggaaatcagc gagggattga cgtaacagcc
    12001 atatttgcgc tcaagtgtgc cgccggaact gatcagttga atgcgttcgc gtttaccgag
    12061 ttcatggcaa atcggatggc tattggtgaa gacctgaatg ttgatgtcag gcaactggcg
    12121 tgccagatac cagcaagttg aactggcgtc taaggctatc accatccctt cttcaatcca
    12181 cgcgagcgcc tcgcgcgcga tatctgcttt atgcgcataa tggcttttca gccggatgtg
    12241 aaagggatcg ccactgtctt gattttgacg gtggatatat ttagcgcgtc catgattgcg
    12301 cagaatttta ccctgcgtct gtaattcatt gagatcgcga cgaatggttt ctttactgac
    12361 ctttagctgt tcagagagag cttccgtggt caggctggta tggttcagca gcaggtcgac
    12421 tatcgcttgc tggcgtgccg ctttcatatt tttcacccca tcattcgata accgcccgcg
    12481 tcatgcgggc gtaccggtat gagattacgg tgttacccct ttttttaaaa gaatattccc
    12541 gtacttcgct cgttcgcctg tgataacgat cgcaaaggct ttttgcgccc gttcataaaa
    12601 cgcaaaacga ttgatgcgga tgatgtcagg acacggggct tgtagtgaaa gcgcattacg
    12661 gtaacgtcgt tctacttcag gatcgagagt gtcaccttct accgctgcca tcatcaccag
    12721 cggcggggca taactgtcca gctcaaataa cgggataatc gcctggagca agtcgctcac
    12781 caacaggcca tcagcgcgga tcacctgcgg tcccatcgaa tgggcgggaa agtgagcatc
    12841 ggaaaaaata atttcatctc catgtcccat ctctgccagc actttcaata gttcgggaga
    12901 aattaacggc gaaattgttt tcagcatttc acacttcctc tataaattca ggttcagttt
    12961 gcgggtagaa ataacggtac tgataatgaa tctgtgcacg ggcttcttcc gggctgttga
    13021 attcccctac gccataccaa ccgaacagcg cagctcctgc gacggtcgtt tcggcatcgt
    13081 cgagaacttt taccggaata tcaagcatat tggctttaat ctgattccac aatgtgttgc
    13141 gacttcctcc accgactaac aataattcag aggccttaaa gtgcccgatt ttttccagca
    13201 tctgtagatt gcgctgtaat tgcgcagtta acccttccag cgccgcgcga tagaaatgcc
    13261 cccgcgtggt attaagcgtc actccttgcc agccagcgtt ctgacacgac aataaatcac
    13321 actgcatttt tacgccatcc gcgccaggcg cgatcagacg agcttcttca atcaacattt
    13381 gccagggtgt ttcagccgtc cagaacagtt ttctcaccca ttccagcacg ccggatgcca
    13441 gccattgcat acctgggtta tacaaccctg cctggctatc cagttcgcag gtggaaccgg
    13501 cgtactgact taacagcgaa gtatcaacct gggcgctacg gaccattaaa atttcccatg
    13561 taccggaaga gagcacgggt tcattttggt cggcaccagc gccaaaaagg gcgaactggg
    13621 tatcgtgacc tgcggaaatc accggtatgc caacgggtaa gccgagcatt gctgcggcgc
    13681 tgttctgtag cgtaccaatc tgttcacccg cttccaccag acgagggaag agtcggcgtg
    13741 gaataccggt ggcttgtaaa atttgcggac tgaaatcgcg ttgctggata tccagcatct
    13801 ggctggttcc ggccatcgtg atatcagtag tgaattcgcc ggttaaacgg tggttaatca
    13861 gcgacgaaat aaagagccag gcgtgcgcgc gttccagcag ttgtggatga ttttctttca
    13921 accacaccaa cttatataac gtattgaaac taaaggctcc gacgccagaa atagcctgca
    13981 accgctgtgc ggagatcaac tgttcaatat tgtccataac cgctgctgtt cgcggacatt
    14041 tccagctaat gatcggatag agcagattgc cttgcttatc taccagagcg ccatccacac
    14101 caaaggtggt gacggcgata ccgcggatgt ggcattcagt cagttcacta ttgatttgcc
    14161 gacagcaatc agcaaagcgt tgcaaaatgg cgtctaaaga ccactggtgc caggtgttgt
    14221 tttccatcgc gatatcgctg gcattaggcg ttgaggcgcg ggcaacaatt tttccctgcc
    14281 gattaaccgc gatggccctg acattggtcg cgccacagtc gagtaccagg ataacttctt
    14341 gtttcataat cgctcctgca atatagccgg ataacattgc ttatccggct aaccactctt
    14401 gcttgttcag gcccggtagc cggagcgacc gggcatcaca tcagggagta atgtattaac
    14461 gcttgtacaa cgggccgtag ttctggcaag cgcggtaatc ctggccttca atatccatgc
    14521 cgtgcgcagc ccaggcagaa ggacgataca ctttggtctc ttcaacgttg tgcatacata
    14581 ccgggatacg cagcatggag gcgagagtga taaagtctgc gccaacgtgg ccgatggtca
    14641 gaaccccatg gttagcgccc cagttcgcca ttaccgagta cacatccgta aacggccctt
    14701 taccggtgag gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg cgtttgttga
    14761 ggatgtcatg cacatccttc ggcaattcca cgctccagcc ttccgcgatt tgcagtaccg
    14821 gtcccaggcc tttgatgatg ttgacacggg tcatggtgaa cgggacgccg ccttcggtaa
    14881 ggaagcggga agagtaaccg ccgccacgga agtattcgtg gatcgccggg caccattcgg
    14941 tagcggcgag gcaagcgtca gcctcttgct gagagatttc ccagtgcggc ttcatcgtcg
    15001 ggttaccttc gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa ccggagttga
    15061 tcaaatggat gatgccgtgt tctgccagtc catcgagttt atgccccgtt acacgctcaa
    15121 ttgcttctgg tgaccagtag gtacgcacat cggcaaatac ctgagcggtg ccggtgagct
    15181 ggtgacccat tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc acgacaaagg
    15241 gttcgcgcac gccattccag tcaaatgaac tgttaaggat cgcttcggcg gtgtcaccat
    15301 tgggatattg atcggtccag tgacgttgcc cctggaagcc cgcagcgatg gcgttgtagc
    15361 caagtgattc ttccacgcga ccaatatcgg ccagtttgct gttgccttgc atcatgtcgc
    15421 ggatacacat cgccatcagt aaactttcgc gcagaactgc gcggcgttgc tcggcattgc
    15481 gttgatactg tttgttattt tcatcttcgc catagcggaa gtttttatca gcccaggcca
    15541 gtgccatttc cagttcggct tcgtcgtaaa tcttctgatc gatacggcga cgcagttcgg
    15601 tcatatccac cgcctggact ttcattccca gccaggattc aaagaagttg tgatcaacaa
    15661 tggaaccggc gatacccatc gaaacgccgc ccagcgacag atagctttta cctttcatgc
    15721 tggcgacggc caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca tcggcaggaa
    15781 tcgatgtgtc atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc gggatgcctt
    15841 tctggctgtg agctgccaga gccgctgcca ggtaaacagc gccgggacgt tcagtgccgt
    15901 taaagcccca aatggctttc gggcgggttg gatccatgtc gatggtttca ctgccatagc
    15961 accagcaagg cgttaccgta atggtgaggc ctacattctg actgctgaat ttttcttcgc
    16021 aagcagcggc ttcagccata cccgcgatac aggtatcgga aatgacacac tcgacggcag
    16081 ctccgcaggc atggcgcagt ttctcggtca gcagtgcggc cgtagctttc gccatattca
    16141 ttgtttgttc ttcaagcgac tcacgaacac ccatgcgacg accgtcaata accgggcgga
    16201 taccaatttt cggtaagctg atttttttca ttacagattc ctcactttat tcggaaaata
    16261 tgttcagtta gttgccgttt gagagcggaa acgggcaaag ataaagatga ccgcgaagca
    16321 gagtgcgggg atcagttcag cagtggggat gttgcccgcc gcgtcactga caaaacccat
    16381 gaccggagtg acaataccgc cgccaatgat ggtcataacg atgaaggacg aaccgtattt
    16441 ggtgtcctgg ccgagattct taatgcccag cgagaagatt gttgggtact gaatcgacat
    16501 aaaggcgctg cataaagtca gggctattaa gcccacatga ccgccagcga aggctgagat
    16561 caggcacagt gccatagcga ttaatgcgta ggcggccagg actttgtgtg gtgcgaagcg
    16621 actgatgagc caggtaccgg tgaaacgacc aataaagaag cacaccatgg ttccggttaa
    16681 atagttagcg gcaaagcctg cagtcatacc tggaatttct tctacagcgt agcgaatcaa
    16741 atagctccag caggccgttt gtgcgccaac gtagcagaat tgcgctaata ctgcccagcg
    16801 ccagtggcga atacgcgcca ggcgagaaag cgatgcggag aacgatcctt gtttggcgtc
    16861 actgtgatta tcactctgca atgccgggaa tttcgtcagc atgatcagca gggcgaccag
    16921 taacacgata gccacgatga tcatataagg tgtctgtacc gataatacca ggctgtgttt
    16981 atacgcactc aattgctctg gagacatttt atcgagaacg tcttgcgatt gatgtggcac
    17041 gttagacaaa ataagacttt gcccaaagac aaccgcgata attgcgccaa acgagttaaa
    17101 tgtttgcgca agatttaagc ggaagtgacc actgctttcc ggccctaata ccgtaacaaa
    17161 agggtttgcg gcagtttcca gacaacctaa tccggctgca ataataaata ggccaactaa
    17221 aaataaggtg tagttcatta tttctgcagc gggccagaat aatgcagcgc ccaaggcata
    17281 taaaaataat ccggtaataa tccctgcttt ataactgagt tttttcatca atatcccagc
    17341 agggattggg ataatgaaat aaccaaagta aaaggccgat tggatcaggc cagcctggaa
    17401 atttgtcagc gtaaaagcct gctggaattg aggtaataaa atgtcgttaa ggttattggc
    17461 taccgcccaa agaaaaaaca gtgagcacag cagcgcgaat ggaataatgt aacttctgct
    17521 ttgccctgca tctttatcta ccgcacggta actctgcgtt tgtattgatg tgtttcccat
    17581 agcatcctct taggttcaga agcttaagca cgtaatcggt tatgttttca aaagtgcaaa
    17641 accagcgagt tcgggcttca tatcagtgaa tatttgcctg tctgaaatga actcatggat
    17701 tcattttcaa ataaaaaacg ctctgtaata tgacggcggt cacacttatt agctggaagg
    17761 tgactttatg tgactaccat cactttaata atcgaaattt catgcccgtt tcagaaaata
    17821 aatcataaaa aacacaaacc agatgaccgt attaattgtt tatgccgaaa acgggcattc
    17881 ctggcttgag tgaatagaaa aatgaccgtt ttgataaata tttttaagag cggtcatttt
    17941 taatggggtt ataaaaagag aacttgcagt tatttactgt gattagttga accaggtcac
    18001 aaaatataag cttgtgaatt tctttctggt taatcaggaa gaagatggtg ggtaattaaa
    18061 cggctaattc aatagtgtga aaggaacaac attattgccc tgttttgaat cagagagagg
    18121 tagctaatgg aacgaaataa acttgctcgt cagattattg acacttgcct ggaaatgacc
    18181 cgcctgggac tgaaccaggg gacagcgggg aacgtcagtg tacgttatca ggatgggatg
    18241 ctgattacgc ctacaggcat tccatatgaa aaactgacgg agtcgcatat tgtctttatt
    18301 gatggcaacg gtaaacatga ggaaggaaag ctcccctcaa gcgaatggcg tttccatatg
    18361 gcggcctatc aaagcagacc ggatgccaac gcggttgttc acaatcatgc cgttcattgc
    18421 acggcagttt ccattcttaa ccgatcgatc cccgctattc actacatgat tgcggcggct
    18481 ggcggtaatt ctattccttg cgcgccttat gcgacctttg gaacacgtga actttctgaa
    18541 catgttgcgc aggctctcaa aaatcgtaag gcaactttgt tacaacatca tgggcttatc
    18601 gcttgtgagg tgaatctgga aaaagcgtta tggctggcgc atgaagttga agtgctggcg
    18661 caactttacc tgacgaccct ggcgattacg gacccggtgc cagtgctgag cgatgaagag
    18721 attgccgtag tgctggagaa attcaaaacc tatgggttac gaattgaaga gtaatttcgt
    18781 aaagcaacaa ggagaaggat gatggctaac agaatgattc tgaacgaaac ggcatggttt
    18841 ggtcggggtg ctgttggggc tttaaccgat gaggtgaaac gccgtggtta tcagaaggcg
    18901 ctgatcgtca ccgataaaac gctggtgcaa tgcggcgtgg tggcgaaagt gaccgataag
    18961 atggatgctg cagggctggc atgggcgatt tacgacggcg tagtgcccaa cccaacaatt
    19021 actgtcgtca aagaagggct cggtgtattc cagaatagcg gcgcggatta cctgatcgct
    19081 attggtggtg gttctccaca ggatacttgt aaagcgattg gcattatcag caacaacccg
    19141 gagtttgccg atgtgcgtag cctggaagga ctttccccga ccaataaacc cagtgtaccg
    19201 attctggcaa tccccaccac agcaggcact gcggcagaag tgaccattaa ctacgtgatc
    19261 actgacgaag aaaaacggcg caagtttgtt tgcgttgatc cgcatgatat cccgcaggtg
    19321 gcgtttattg acgctgacat gatggatggt atgcctccag cgctgaaagc tgcgacgggt
    19381 gtcgatgcgc tcactcatgc tattgagggg tatattaccc gtggcgcgtg ggcgctaacc
    19441 gatgcactgc acattaaagc gattgaaatc attgctgggg cgctgcgagg atcggttgct
    19501 ggtgataagg atgccggaga agaaatggcg ctcgggcagt atgttgcggg tatgggcttc
    19561 tcgaatgttg ggttagggtt ggtgcatggt atggcgcatc cactgggcgc gttttataac
    19621 actccacacg gtgttgcaaa cgccatcctg ctaccgcatg tcatgcgcta taacgctgac
    19681 tttaccggtg agaagtaccg cgatatcgcg cgcgttatgg gcgtgaaagt ggaaggtatg
    19741 agcctggaag aggcgcgtaa tgccgctgtt gaagcggtgt ttgctctcaa ccgtgatgtc
    19801 ggtattccgc cacatttgcg tgatgttggg gtacgcaagg aagacattcc ggcactggcg
    19861 caggcggcac tggatgatgt ttgtaccggt ggcaacccgc gtgaagcaac gcttgaggat
    19921 attgtagagc tttaccatac cgcctggtaa atgcgctgat gtgataatgc cggatacgac
    19981 gtttgcggcg tatccggctc gccgttaccg taccaaccgc aattgctgta aattgccgtc
    20041 gatatgcaaa tcggtttgta agcgggcaat atcgcggcac agaaacgcca tctctttatg
    20101 ggtttctaat tttttgcgcc acttttcggc aaccgcatcc agattctcat atatcccttc
    20161 cagactctga aactcgacca gcagctgcgt ggcgcttttt ggtccgattc ccgcaacacc
    20221 cggtaccttt gaactgctga tccccgcaag tccccagtaa tcgggcaact gctgcggttg
    20281 aacgccaaat tctttatcga taaatggcgc atccagccaa cgtttctgga agtaatcacg
    20341 aatacgtaat gtcggtgaaa gtaactgaca gtagccttta tctgtcgaaa caatcgttgc
    20401 ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg gctaagtcat cggcttcgtt
    20461 gccgctggtt gaccagcacg ggacgccgcg ttgctcaaag gcggcgcgta atgcaggcat
    20521 ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct ttgtaatctg gtaaacgctg
    20581 atgacgccag ccgctactgc ggttttcatc atcaaaaacg gcgaccgcgt gggttggctg
    20641 gctgtgcata atgagctgat cgagcgcatg ctggcaggtt tcgacacagg gcgatccctg
    20701 aacggcatga atgcgacgaa taagattcag tgcatcgaca ataagcaaat gaacagccac
    20761 gataaccccc ttaaagatca ggcgataagg gtaacattcg atgccggaac aggctacggc
    20821 tggatgagaa atcgggaaga ggcctcgcaa aacgaggcct ctggagagcg attaatcgca
    20881 ggcaacgatc ttcattgcca ggccgccgcg agaggtttcg cggtacttgg cgttcatatc
    20941 tttacctgtt tcgtacatgg tttcgataac tttatcgagg cagacgcgcg gctcgctggt
    21001 acggcgtagc gccatacgtg cggcgttcac cgcttttact gccgcaatgg cgttacgctc
    21061 gatgcatggc acctggacct gtccggcgac cgggtcacac gtcagaccga ggttgtgctc
    21121 catggcgatt tccgccgcga tgcacacctg cgccgggctt gcacctaaca gttctgccag
    21181 accagccgcc gccattgagc acgccacacc aacttcaccc tggcagccga cttcagcacc
    21241 agaaattgac gcgttcatct tataaagaga accaatggca ctggctacca gcaggtaacg
    21301 agccagtgag ttagcgttca cttcgcggat aaacttgtcg tagtacgcca gcactgccgg
    21361 gataatcccg cacgcaccgt tagtcggcgc agtcaccacg cgtccgccag cagcgttctc
    21421 ttcgttcact gccaatgcaa acatgttgat ccagtcaaca accgccatcg ggtcagtggt
    21481 ggttttatcc tggctgacca gcatccggcg tagtgccgca gcacggcgtg gaacgcgcag
    21541 tttgccaggc aacacgcctt cggtggaaat accgcgctca ataccgccac gcatgacttc
    21601 ccatacgttc gccaggtgct gttccagctc ttctttgctg tgcagcgcca gctcattttt
    21661 catcatcagg ccagagagtg acagcccggt ttcctgacaa tgtttttgca gatcggctgc
    21721 tgaactgtac ggataaggaa cttcaaccgg tgcgctattc tgctggccaa aatgctcttc
    21781 atcaacgata aagccaccgc caatagagta gtaagtctgg ctgtaaacga ctttatcgcc
    21841 cgccagcgcg gtaatgcgca taccgttttc atgcagagaa aggttgtctg catggaagtt
    21901 catgcactga tcaaccggga actccacttc atgctgaccg tttgccagca tcaggcgacc
    21961 atgagtattc acatcctgaa taaaaccagg gatggaatcg atatccacgg tatccggcag
    22021 gttacccgcc aggcccataa taatggcgat atcagtgtgg tggcctttac cagtcagaga
    22081 gagcgagccg tacacgtcaa ccaccacgcg ggtcacgtct ttaagcaggt tacgggcaat
    22141 cagatcgtcg gtaaattgtt tacccgcttt cattggtcca acggtatgag aactggaagg
    22201 gccaatgccg attttgaaaa tatcgaatac gctaatcata ggaaatacat cgcgttaaaa
    22261 cggaggaagc gccgcccgaa agcggcgcga aaggacttag ctgaacagag agtagaagat
    22321 tgcggagatt gcaatcagac ccatcacgac aacgaatacg ttgctgatgt gaccgctgta
    22381 cttacgcatt gccggtactt tctgaattgc gtacatcggc atcaggaaca ggatcatcgc
    22441 gatgattggg ccgcccaggg tttcaatcat acccaggatg ctcgggttca gggtggcaac
    22501 aatccaggtc gttaccagca tgaacagcgc agtgatacgg ttcagcttgt tgatttcgat
    22561 agacttacct ttaccacgca gagatttaat caccataccg ttgaagcctt cacgtgcgcc
    22621 caggtagtgg ccgaggaagg atttggtgat agcgataatc gcgataatcg gagccatcca
    22681 cgcgataacc ggtgcgttaa agtggttagc taggtaagac agaatcgaga tgttctgctc
    22741 tttagccgca gccaggtctg ccggagtcag gctcagtacg cagctgaaga cgaagaacat
    22801 tacggtcagc accatcatga tgtgtgcgaa tgccaggatc ttcgagcatt tctgttctgc
    22861 catatcgccg tactcttcac gcttcgcaac ggcgaaagaa gagatgatcg gagagtggtt
    22921 gaacgagaac accattaccg gaattgccag ccacagggtc atccacagac cgtttccggt
    22981 tgcagatgca gtgtccagag acagcgtttc cagtgctgcg ccgttccact gcgggatcag
    23041 gtacagagcc agcagcatca gtacgccaac aaacgggaat accagaatac tcatcgcttt
    23101 aacgatcatc tgctcaccaa agcgaacgat ggtcatcata cccacgatca ggatcagcga
    23161 cagaatcgca cgcggcggtg gcgtcatacc cagctggtga gacatgaagc tttcaactgt
    23221 attggtgatt gccacgctat aaaccagcag gatcgggtag atagcgaaga agtagagcag
    23281 ggtaatcagt ttacctgcgc caataccaaa gtgttcttct acaacctcgg tgatgtcttc
    23341 gcccgggttt ttaccagaca gtacgaagcg agtcaggccg cggtgagcaa aaaacgtcat
    23401 cgggaacgca aggatagcca tgatgatcag cgggatcata ccaccaacac cggcgttgat
    23461 tggcaggaac agcacgcccg cgccgattgc cgtgccgtaa aggcccagca tccacatggt
    23521 gtctgtcttg cgccaggcac tacgagagtc tttcgacgca atcgtgctgg tttgagtcgt
    23581 ttccatctat ttctcctgga ggaatgcaaa taattcagct tttagccagt tcagcccggg
    23641 aaggtgcctg accgtaaaaa tgaaatctgt ctatcggcga tggcttaatc atttaccgcc
    23701 gtaataattt gagggcggaa agatacacac aagttatgta tctatcagtg atcttgatcc
    23761 caaatacaat aatctacttg gaagatagat tatcccaacg ctttactctg taaatcaaaa
    23821 gtaaaagaga atttaagggc aatatttgcc cattaaacga catctgac
//
LOCUS       DAZFRJ010000059        22830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000059 DAZFRJ010000000
VERSION     DAZFRJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.059"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..2134)
                     /locus_tag="ACHKUN_002126"
     CDS             complement(11..2134)
                     /locus_tag="ACHKUN_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO6660377.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            2256..2522
                     /locus_tag="ACHKUN_002127"
     CDS             2256..2522
                     /locus_tag="ACHKUN_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO6660378.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            2593..3078
                     /locus_tag="ACHKUN_002128"
     CDS             2593..3078
                     /locus_tag="ACHKUN_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660379.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(3093..4937)
                     /locus_tag="ACHKUN_002129"
     CDS             complement(3093..4937)
                     /locus_tag="ACHKUN_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO6660380.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(4937..6343)
                     /locus_tag="ACHKUN_002130"
     CDS             complement(4937..6343)
                     /locus_tag="ACHKUN_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO6660381.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(6353..7045)
                     /locus_tag="ACHKUN_002131"
     CDS             complement(6353..7045)
                     /locus_tag="ACHKUN_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO6660382.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(7048..7503)
                     /locus_tag="ACHKUN_002132"
     CDS             complement(7048..7503)
                     /locus_tag="ACHKUN_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO6660383.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(7503..8351)
                     /locus_tag="ACHKUN_002133"
     CDS             complement(7503..8351)
                     /locus_tag="ACHKUN_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO6660384.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(8351..9769)
                     /locus_tag="ACHKUN_002134"
     CDS             complement(8351..9769)
                     /locus_tag="ACHKUN_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO6660385.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            complement(9778..10260)
                     /locus_tag="ACHKUN_002135"
     CDS             complement(9778..10260)
                     /locus_tag="ACHKUN_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO6660386.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            complement(10235..10420)
                     /locus_tag="ACHKUN_002136"
     CDS             complement(10235..10420)
                     /locus_tag="ACHKUN_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660387.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            complement(10463..11734)
                     /locus_tag="ACHKUN_002137"
     CDS             complement(10463..11734)
                     /locus_tag="ACHKUN_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO6660388.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            complement(11746..12630)
                     /locus_tag="ACHKUN_002138"
     CDS             complement(11746..12630)
                     /locus_tag="ACHKUN_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO6660389.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKPAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            complement(12644..14770)
                     /locus_tag="ACHKUN_002139"
     CDS             complement(12644..14770)
                     /locus_tag="ACHKUN_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO6660390.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDLMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYGKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVGVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            complement(14773..16185)
                     /locus_tag="ACHKUN_002140"
     CDS             complement(14773..16185)
                     /locus_tag="ACHKUN_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO6660391.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            complement(16182..16607)
                     /locus_tag="ACHKUN_002141"
     CDS             complement(16182..16607)
                     /locus_tag="ACHKUN_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFO6660392.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            complement(16687..16929)
                     /locus_tag="ACHKUN_002142"
     CDS             complement(16687..16929)
                     /locus_tag="ACHKUN_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO6660393.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            complement(17033..17404)
                     /locus_tag="ACHKUN_002143"
     CDS             complement(17033..17404)
                     /locus_tag="ACHKUN_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO6660394.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(17688..18206)
                     /locus_tag="ACHKUN_002144"
     CDS             complement(17688..18206)
                     /locus_tag="ACHKUN_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6660395.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            complement(18412..18849)
                     /locus_tag="ACHKUN_002145"
     CDS             complement(18412..18849)
                     /locus_tag="ACHKUN_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6660396.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(18846..19322)
                     /locus_tag="ACHKUN_002146"
     CDS             complement(18846..19322)
                     /locus_tag="ACHKUN_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO6660397.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(19306..19629)
                     /locus_tag="ACHKUN_002147"
     CDS             complement(19306..19629)
                     /locus_tag="ACHKUN_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO6660398.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(20702..21325)
                     /locus_tag="ACHKUN_002148"
     CDS             complement(20702..21325)
                     /locus_tag="ACHKUN_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6660399.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(21322..21987)
                     /locus_tag="ACHKUN_002149"
     CDS             complement(21322..21987)
                     /locus_tag="ACHKUN_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO6660400.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQG
                     EGA"
     gene            complement(21965..22171)
                     /locus_tag="ACHKUN_002150"
     CDS             complement(21965..22171)
                     /locus_tag="ACHKUN_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO6660401.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            complement(22168..22773)
                     /locus_tag="ACHKUN_002151"
     CDS             complement(22168..22773)
                     /locus_tag="ACHKUN_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO6660402.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(22766..22813)
                     /locus_tag="ACHKUN_002152"
     CDS             complement(22766..22813)
                     /locus_tag="ACHKUN_002152"
                     /inference="COORDINATES: protein motif:HMM:NF017611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO6660403.1"
                     /translation="MSDPNSSMYEEEYDG"
BASE COUNT         5403 a         5693 c         5205 g         6529 t
ORIGIN      
        1 cataggtctt ttaaaagttt ccctctgata agaaagaata ggttcctggc ccacctggaa
       61 tattgcatgt ccatgccttt ggttgtcctg aagcaggtgc gctattgata acacgctcac
      121 ctatatccca gaaaccggat gtaggtactg acgaaagata aaatgttgtt ttttcaactt
      181 gcaacgcgtt tgatggcatt ccatatgcac atagagaagg tgagtttatg agtacatttc
      241 ctgaaccata atttcttata ccaacattta tagctgtatc agtttcgtat atatacacac
      301 cacaactaac atagctccat ttcccattac cagctaaagg tgctgtagag tctgtttgtc
      361 ctgcaccatt aattataaac ataagttttg acgctgatcc ctggtcattg acccacgcac
      421 caaaccatac aaatttccct tttagttctg gtgatttgtt gaggtctatc gttcgtcccc
      481 aaactctatc tcctgtcgta actaagaatg aataattacc agcatagatg ttttgagttt
      541 ttatcaatgt tcctgtagtt ccagatgccg cagtccagag caacatattt cgatcaagat
      601 agttagcagt taactctctg ttcgcaatat ctattaaatg cgcagtgtca gggttttgtg
      661 tcgccgggtc ataactacca acgtatccga tggagttcac tgtgttgttg gcaagatata
      721 gcctgtcagt aactttggac gaaagattat tgtggtacat tgctaataaa ttattcactt
      781 tcgatgcgtc taataactga ttattttcaa tcgtcaggtt tttcgcatag ttcgtgaaaa
      841 taaatccgtc ttggttaggt agcgcgctgg cgtagccaat cgcggtctgc tgattcccgc
      901 gaatagatac ggtatcatta atttttgaaa ggtcaggaat taatgtaaaa ttgttcgcga
      961 taaggtggat atccgcacgt actgtaatag actcaggtac actatacggg taaccaatct
     1021 caccgttgcg ttcccaatag ttagcatcaa ggttaaggcc tttaaccccg taagccaaaa
     1081 gtccgtagaa cttattttgc tctatattgt tgccattaac tctgacgctc ataccgccct
     1141 cagcgtaagc tggcgtaagt aaaactccac cggctccgtt accatagata cggctgttga
     1201 ttatattaat attattgcag taaccaccaa cattaatacc gttaccggcg ttttgaaaca
     1261 accggcaccc aatgatgtca atggagtaac cgttaactaa tattgcgttg ttattagtat
     1321 taaccacgtc aatattttct attttaatgt ggttggcttc agtaagactg atgccgttag
     1381 ctgcgtagcc atttccgtca agaccaaagt ccctgaactc gcagaacgta attaccccat
     1441 cttgttgcgt gattatcggt gccgctgtac ccgtagagaa aattctggac tgatatgcgc
     1501 cagcacctcg tatcatggtg acagtattct taatcgtaat accagggtca acaatgaacg
     1561 ccccctctgg tataacaatc tcagttggca acgtagcatc atcaatagcc ttctgaaatg
     1621 cagctttgtt ctgagcaccg gtgtttccct cagcaaaccc gtagtctgta agcgtcctca
     1681 ggtcgatagt tagctttttt gagagaatgg tcgatgtgct atcgacaaac tgtgggttat
     1741 gcatgactag agagtcgcca agtgagtgat cttcgctcag gagattgccc ttaaactgat
     1801 ctggatcata ctttagcaca ttcggaaaat agaactgctg cgcaccgtat gcatcataaa
     1861 cagccataga atggccttgc acagttacga acttggcaat ctgtccgtta tatacagggt
     1921 aaccagcagc gttaatgatg attggttgcg aaacaggaac gtgagaaccg tcttcgtttt
     1981 ccacataaac ctgaatctgg ttttctggat ttaccgggtc agtgtcaatt ttaccgatat
     2041 aaatttttcc attggctacg gctttaaaag agcgagacat agtgaagagt tgcgaaggca
     2101 tgctgaccac aacatttgcg gtgatatctg acatttcatt gctccagaag aatgatatga
     2161 tgcaaccatg atgtgattgc ataccgaaat ggtactattg agtatttatc cagtaggtta
     2221 cgatgccatt ccacccaact ggtgaggcat caaggatgta cagcaaatac gacgaggcgc
     2281 agtttcactt gagacttccg catgaactcc acgcgaaaat taaacagcgc gcgaagatga
     2341 ataacaggtc gctgaactca gagataattg cagcgattga agaatcgttg gctaaacaaa
     2401 gctctgcatc cgtttacatt gatgatgcag agcgtatggc agaacaacaa tctgatatgg
     2461 ttaagaaaat ggtttttgaa acgcttaaga ccatgtatag caataataaa aaggaaacat
     2521 agaaatctag tttccggcta aaatggcatt gccttcatga tatcctgtga aaaactaagg
     2581 agagttaacc atatgaaaaa atcactgtta attatcccgc ttctgctggt tggatgcgca
     2641 aaagtaagtg actatcaagc aagttgcgaa caacgctatc aaaagcttag cgatatggct
     2701 aattgccttg atgccagtgt gaagaacgac tcacgcatgg catcagcacc aacacctaag
     2761 ctgtatgtcc ttgctgcaaa gatgctcggg aaaggtgtcg atgaaggcaa gataagtgac
     2821 gcacaggcaa gacttgagct tcagaatctt tatgttcaat tacaaagcca agaacaagcc
     2881 caacaaatag cacaaagcca agcattccag caggctttat tgaattatca ggctgtaaac
     2941 acaatgcaag cgatcgagca aaaagcgcgc cagcctgtta taactcaacc ttacccaaca
     3001 cgcgttgaca catatacaaa ctgcaattca ggatttggaa acacggtaac atgcaacagt
     3061 agcagtaata tcaggtagtt acatcttatc ttttattgct gttctgctgt aatttggctt
     3121 gagagtatag ggcgtatcgc atttgcagca ttatttagcg ctctttcata ggctggtgtt
     3181 cctgctttgg tgtttgccaa acgtaagaga gcattccttg ctgctttgga ctcatacaag
     3241 cgcatcattg caccgaaacc agcctcaagc cccattgata cgccaagagt cgcagttgcg
     3301 ccaatcgtcc ttatcctgtt ggcttgcgat tgcccagtct gagttactac atttgcggtg
     3361 tctgaccttg ctgttttctg tagaacttca tgaagagaat caagctcttt catgtgcttt
     3421 ccagaaaaaa tagtgttgta aatttcacca cctgactgag atttcagctt attaacttca
     3481 gtgatgaact tggctggaga gtcaccggcc ttttccgcta ttttgctgac gtaagctgca
     3541 cgcatagcat ctttcccttt atcatccaat gcgctccaga ttcgtttcac gtcagatggt
     3601 tttctgctta atacaacagt atttataagt tcaggactgg cttcactgct tgccttgttg
     3661 agcttgttag caatgttttt attaagcacc ttattataaa cgtttgcata atcggaattt
     3721 gctttaaggt attttgctgc gtctgacgca ccgaggtttt ttgcaactgc gttacgaagg
     3781 tcttttgaca ttgcattctc taccatattg gtagctgctt ttgcctggtt ggggaagacc
     3841 atagcatctc cctgaacatt agatctaaat gctgttctgt gctgacgcaa gagatcaaac
     3901 gtaacatcca aatcagttgc agggtttgct aattcttcac gcaggttacg caaggatgta
     3961 agcaggcttt gattggctga agtcccaagc cgttcctgtc ttgcgatcgc tgtattcaga
     4021 gcattcatgg tatttgtagt atcaactgcg gcattaccca ttttattggt gacgtcattg
     4081 ataacagcgc cagcagcatc cttccgcccc cttaacgtgg tggtcagaga tttcaccaca
     4141 tcatcagggt tgtactcacc aaaacggtca aaataattgc ttaccagctt actacgcgtt
     4201 gcatattgct ctgctcgctt tgagcccgtc ccgagcaaag ccccctcggc atcctgagta
     4261 aggccgcgag tgaaagcatt tttcggcggg ataacatcag atgtcattgg tgtcacgccc
     4321 atcgattctg atgtggcaat tttcttcgcc acttctggcg caatatcacc ttttatagcc
     4381 gttattccac gccctattcc ctttgctgct gcggaaagaa caccctgagc ggcaaggtta
     4441 actccggcat ttttagctgc attttgtgcg aaatcgcctt tctgatttgc ggcctctgcc
     4501 agcgatccaa tagccatgct tcctgccgtt ccaactcctg gaactaaata cccaccaatt
     4561 gtttcaccgg cttgtgcgta tgggtctgtc ggtttgtcta ctggacgata aacatcatcc
     4621 aaaacttttg gcccaccaag cccctgactg attgcattaa tcagacttgc gccaccctgc
     4681 aatacgtcaa atggtatgtt taccagacca cgaccagcct gttctgcaat ttgccctgca
     4741 ctttgaccac cagtgagcca atcgccagct tgttgcatca atgatggttc gtcccgtgtt
     4801 ggtgcattat tggcctgatt aactgtttgt tgctgaacag cctgaccagc aaaatactca
     4861 tcaatggcgg tgccaatatc ttcggtgctc gtaccatcag gaaaggtaaa tgtcttaccg
     4921 tttgcagtta ctttcatcat tccaccgtaa attgaatgcc tgattttgag gtatatgatc
     4981 caacctgatt ccgtggttct cctgaaggtg tcgaatcttg tgctggcgct gcgtcagtat
     5041 tcattgacat ataccgctta acggcactcc ccaatgattc acctttttta acatccaacc
     5101 ccaatatctg accgccatta cgcgattgtc cagggttgcc attcgcgctc atccactcgg
     5161 ctttaaactc attaaactgc gcgtttcgtc gctcaaggtt tgccattgca tcaagccatc
     5221 ttgcgaccgt ctcagggtta tccatgtcag ttggcgcacc ctgtcgaacg atctcaacgt
     5281 ctttatccgt tgctgggccg ggaggtagga atttaagaac ctgactgtta acaagggcat
     5341 tttggcgaat gcgcaaatca cgcaatgttg tatcgcttcc ggtaagtttt gcgaacatgt
     5401 tctgtgcgtt accgaacaaa cctgtcgttg gtttttctgc tctgaactgt tgagcaagcg
     5461 cactcataga attggctgag tttgatgatg ctgtagcatt gtttacagcc gtctcgatgc
     5521 ctttttccat gtttactgac agcttaggtg cttcgctaat caactgctga gccttttcct
     5581 gcgcttgctg catcttaaac ccgaactctt gctgatccag agccaagcgt tgtgctgcga
     5641 tattgtgccc agtcattgct gactgatagg aaaggttttg ccctctcgcc tgaagtgctt
     5701 ctccagcctg attgctgcgg attgtctctg ccagtctgcc tcggtcaatt tcacgaccag
     5761 ccatcttgtc ctgaacattg aagtaatcaa ttggaccaag agcagccatt ccaaggtgat
     5821 caacaaactc accaaatcct gaagggttct gctgatacat ctgagcaacg ctgttagggt
     5881 caacaccgac gcgagtcagt tccttggcgt tgttttgcag ccatgattgc attgcttctg
     5941 gagacgatga cgcaaggcgt gcgccagccg ctaaggtgcc gatagaatta cgctggtctt
     6001 catcaatgaa tcccatgcct ttacgaacgg attcaatctg gtctggatat tgagtagcca
     6061 actgacgcaa agcaccgcga tcaccagacg cataagcatt agcgtacgcc tgctgaaatt
     6121 ctttctgccg ctgagcctgc ttttcctgct gaaacacccc tgcaatacct gaaaggcctt
     6181 gcaaagcagt cagcccaaca ttgttagcgc ctgaacgatc aatatcattg ttctgcctga
     6241 taagctgaag cgtattgccg atgtcattta cgctcggagc gtttgagttg acgccgccga
     6301 taccagccaa caatccgccg tttgttcctt gccaagtagc catgattacc ccttaaaaca
     6361 acgagccaag caatccgata ccagcaccaa tgccagcgcc ccaaggcgtt gatgttccca
     6421 aaaggctggc aagacctgca ccggcaatcg caccagatgt gccgccgcta attgcagtct
     6481 gaagacttga tggtttattg gcattagcag cggcaagtgc tgcgctttgc tgtgcaatgc
     6541 tgctcatgtt gttggcgtac gtctgcccgg cgtttgcctg accttgcagt gcaccaagcc
     6601 caacgtttgc cagattgttg taattgctca tctgatttga taaccaagac tgaccgagtg
     6661 tcggcgcgat cgtagccagt tgattgcttg tggctgtcga accaagtcca cccgtcgcct
     6721 ccgcagcagc aagactctgg taacgagcct gacctgcaag gtctttatac tgctgagagt
     6781 tgtaatactg attaagtgcc tgcccctgac cttctaaact ggaaagattc tgaagctggt
     6841 taacatactg ctccgcaagc ggcgtgaacg gagcaaggtt tttcatgatc gtctgccact
     6901 gctgattttg caggtctgcg gcatacttct gagcttctgc tgcatacttt gcgcttttat
     6961 cagagctgcc acctttcccg cctttttcag ggcaataagg ttcctcgccg cgcagttttc
     7021 tgcccagctt aaatgcatat aacatagcta tctcccgtga ttcaggaagt cgattagttc
     7081 ttcgcgtgtg gcgctgtaaa acgtcacgtc atccacgcct ttgaagtatt tcttgatggt
     7141 tcctacacgc ttaaggccaa tcattgcgca gtacatctga ccgtggcgga atttgcgcgc
     7201 agcgaacgat gtgacgcact gaacggtggt gttagtcaga atgtatcgcc agaacgccag
     7261 cccgatttcc ttgctgaatc cacgaacctc tggcaggtac atggcgtggc aatcgaatgt
     7321 aagcggctga atttcctgat agtaaacaat gccgccaaac tgaccgtaca cgttaacctc
     7381 aaagtaacgg cattcaggct tgtagtcgta tccatcaccg ttgttgcttc cggcaataat
     7441 gtcagggtga tttccgactg cttctatcag gtcgatgttt cgcgttggtt tgaactgaat
     7501 cattactgct ccgcgattat cttgatggtt gtggcagtaa acgccgcacc atttgactga
     7561 atggttaacg tgctgccatt tgtggcaaga aagccgtctt tatccacgct gaagaacgta
     7621 gctaacagga tgttatcggt cgttgtcgcc gagttgcgac tgcttaccag tgtgtcagga
     7681 acagagccgg aaaatgttag ttgcattgac ctgttggcgg ttccgctggg ccacgtcccg
     7741 acgatcgaca gcttgaagaa caaggttttg ttctcgttga acacaaccat cttgttgtta
     7801 acggtgtcga agaatggtgc caacgagcct gatgacggcg tgagcgtttt cagcaggcta
     7861 acaaggttgg tcggcgctgt cgggatggtt acagatacgc cagagtaaac aacctctgac
     7921 ttcttgcgag tagtggcata ctccagagca tcaatgcgcg attcatggtc tgaaacctgc
     7981 gattccagcg actgaactct ggtatcaagc gacgcaatat cgctttcatt ctgagcgatt
     8041 cgcgtttcat gttcctgaag agttgattct gcctggctga ttcgctcctc atgattaaca
     8101 agcgttgctt ccgcagcaga aattcgctgc tcatggtcag cgagaatcac atcctgctca
     8161 tcgttcctga cttgtgcgtc ataagcgccc tgtccggcct cgttggcctt gttagccaca
     8221 ttaccaacat cagtaccctg tgcgataacg taaagcagat acgactgcga gaagatattg
     8281 cgtggaagga ctgatgtgtc gagccgtgta gcctgaatga ttaccggcac attgagattc
     8341 gaatccgcca ttactcaatc cttatctgag cgccagacag agtgacaggc gacttagtga
     8401 taacgcgcag tttgaagcca acatttttcc tgatgcgccc tactcgcttc cacaaaacgc
     8461 gtttgtcgta aacgaacggt tcattctgct caatcatctg ctcacgcccg taatttatgc
     8521 cgtcagtggt tgcagagagg aacaggcggt cagcgtactg agcaacaccc gtcgatgatt
     8581 ccacctccag atcaaagcat ctggcgttat ccgctttgaa caacggagta aacagcaggt
     8641 gttcttgctg tagcccatac tggctgctga tatcgaactg caatttcccg gtaaccgatt
     8701 ccagcttatc gccgcacgtt atctgatttc cttcgtaaat gaagtcgata gcgcggtaca
     8761 catcgtcata caggcctgtt ttcaacacac accattgcgg accattggcg cttgaagatg
     8821 cgtcgtacac gaggacgtgg cgcggaaggt ggataatcag cagttcatgc gcatcaaatc
     8881 gcagcgattc catcacacca tcagccagtt catcagcagt gtaggagcga agaattttct
     8941 caatgctcgc gctggcgatt ggtgatacct gaccggagcc gatgatatac acagacggcg
     9001 cacctgttgc cggattgctg atgaacgcat acgaatcggc gaacggcgtt ttgcagtaag
     9061 tcccggcaat gcctttctgc accatcagcg atggctgtgc gacatacaaa gcggcaccaa
     9121 cggtggttgc ccccgtcagg gagaaatatt caatcgtcga tgaaccaaag cagacgatga
     9181 agtctcgcca tgttccgatg ccgatgatgc cgtcaggctg agactcggct cgatattgtg
     9241 cgctgtagcg gtcaggatgc gattcgtctt cagggtcagt gataaaccat gaatcagtgc
     9301 cgtcttttga ccacgcataa cgcccacgta aacgcgtaat gtcgcgaaca gaacctaact
     9361 catactgcat aaacccgctg tctgcaggcc agtttgagac ggttttaacc gtgccatcat
     9421 agcggtattc gaccagttgc ccgttaacgc ctaccgcctg tgatgttcga ccatgcgcca
     9481 ttgatacgcg accacttccg gcaacatcac cgacctcact ttcgccctta tacagtttgc
     9541 caccacacac gcgataaaca gcattctgtg ccatgttgta ctcgacgcct cgcgatacgc
     9601 cgttcacatc agaacgtttg gcaatgcccg ggaatgagcg aagatatccg ctgctgttca
     9661 ggatttcttt gggtgtagcc aacatattca ctggcagata gtcgatatag tcggcgtttc
     9721 taaagtcttt gccgacacct ttcataagcg gaagttgctg aataggcatt tattcaccta
     9781 tgcgtttggg atatcgccat caatcagagg gagatcgcct ggataatatc ggtcagatgt
     9841 gaacacgtca tatttattac cctgccctac aggaaaatct ccacgtcgtc gcattgaagg
     9901 aacaaccaga gtgtcggtca tcaaggcatc atatgagcgt tgggcgttac tgagaacttg
     9961 cggagttggt tcaaggctgt aatcagatag cattctcagc aataactgat agcctactgc
    10021 gtgtttgtat tttcttggaa gacctgactc atcatctggt aatggctgct catctccagt
    10081 tgcgaaagcg taaccaatgt cgccggggtt aatcatccac tcggacatca tatcttccag
    10141 atcatttaca ccatcttcaa ttgattgcgg ctcaacatca gtaagcgatg cattagaagc
    10201 aatagcaaac ttacgaagcg caaaaaggac gatctcaccc tttgtcagta ctgttgccat
    10261 tgtccgccgc cttacgacct cgcttactgg tcggtttcaa ttcatcaact gaggcaacaa
    10321 agcccaacct ttcgaaaaac tggaagtctt tttctgcgat aacggcctgt acatgcccgg
    10381 attcgttatc tgcggcaagg aatacactca tccgatccat attgtttcct taaaacataa
    10441 aaggggcgta agccccttgt tattacggat taccgaagaa ctgaccgccc atgtgtgggt
    10501 taaagcacac atatgcaggc agtaagtcga agcgcatttt ttgcacgttg gcatcgccat
    10561 ctgcgtattt atgtacgcgg atggagaaac cttcatatgt tgcaacagca gaatcaatac
    10621 tgtgcagttt cggcagtggg atagatccga gtccacagaa gaacttgtta tagaacaggt
    10681 ttggcttcat tgtctggcta gcagtgccta ctacagatac ggcatcgcct gcctctacct
    10741 gacgacttac agagttgtac tgcgggtttg tagtgtcata aatcggaaca ccagaaagcg
    10801 taaccgtcac atcgccactg ctgtctgaat tagcatcagc agtaaccgtt gcagtgaagc
    10861 taattggtgt ggctccgtta tacaacgcct gtttggtctg ctgttgcagc cagtaggtat
    10921 tggtgaattt gacctgatca ccagctttca gaaaacctgt aacgctggct gtcgctccgg
    10981 tcaatgttac agtgaactgg tatgagtctt taactgcgtt ataggtaaca gttggctgtg
    11041 ttttgactgt cagtgttccg ccaaatgccc cctgcgtacg agaggcaagc ccattagaca
    11101 tcagtgcgcg aatgccgcca aaattggttg ggatctgtgc gttctcccat gcagtacgaa
    11161 ccaattgatc tgaagcatgc aaaccagtct gcgcatcagc aagtcgctgt gcagaccatg
    11221 gatccattac agcatagttt tcaccttcat taacgccgag gtctttcagg aaagatgccg
    11281 tctgcgcaac atcagaccat ttggtgattg gagtattggg gctaccaagt gacaacgcac
    11341 cgttattcat catgaagtga gcaagctctg tttcaaggtc ggtaacgatt cgctggcgaa
    11401 ccggcgcgag aatttcttcc agctggttaa gcttgatcgc ttcctccagt tgctgatatt
    11461 caacagcaac agtgatgtag ttacctacac gccccgtagc tttacctgag atcaggttgt
    11521 ttttattttg ccctgaaata tcaccagtgg gagtacggag ggatgagaat tgatgcggac
    11581 gtttaaagct aacgctatcg ccagtgctgg agttgatttc acctgccagc aactgacggt
    11641 ctacggtttt cgccagaact aaatctgaca taaaacccgg aaggaatttt ttcagaacga
    11701 tttgactgac gttactgtcg agattgttag gcatttatct tttccttatt cgatttttgc
    11761 gccggggcat aatttgttga attcgtcttg tttcgcatca gcaccgccac cacgtacttc
    11821 cggctctggc ttgatggctt tctttggttt tggagcaagg cttacctgtt tgctaatctg
    11881 ccccaagagg aatgctgcgc gaattggatc tgtctcagcg gctacacgct ggcgtaattg
    11941 ctggctctta cctaagccat aggcgagtag ttcagagcct tcgtctgcac agtgaatgat
    12001 gatttcctgc tgaattggtg gtagctcact aagaacaatg gcttccattt cctgataatc
    12061 tttcacagga agtttggctg cccgttgttt atgcgcttct accctttgct ggaaacgctg
    12121 ctggtattcc tgttgctgac gtagtttttg ttgctgctgc tgttcgacac ggcctttttt
    12181 ctcatgccaa tcagtcaatg cctgttcaaa cgcctgttcg tcataatcac acgactcaag
    12241 agtcggtttt ggtggaatag cgtctggttg tggttgctga tgttccgcag gcttggctaa
    12301 tgcttcctca agctggcgtc gcaactcacg gttttctttc tgtgtttctt tgaagccttt
    12361 gcgaagatct ttcacccatt gcggtgcagg ttgcccgtca atgtgatcat tatcgtcagc
    12421 gttaagctga atttcttcat caccaatacg caaggcgtaa tcttctggtg tctcttcggt
    12481 tttttcagga tcagttgcca tctcttttcc gttgtcatcc tggctttcat tctcaggctg
    12541 tgactctgtt tggatgatgg tttcttctgc attttcctgt gtttcagaca ggtcaataac
    12601 ctgaccgtcg atgatcagtt cgttttccat tgattactcc tggttaactc ggcattaagt
    12661 ctgccggtga ctgtggtggt gactggaatt gctgttgttg tgactcggcg acatctttca
    12721 gaaggcgtat tgcctccatc actgctttgt catcgatgtt tctggcttga gccagtttat
    12781 agacagtgtt tgcctgactc tccatcgcat cctgctgggc agtaaatgct ttgatttgag
    12841 tttgagcagt ttcgttagtt gctttttgcg cttctgcctg cgctgctacc atttgcgcct
    12901 gagcgagaac catttcagga tttggctggc tttgtgctgc catttgcgcc tgttgaacaa
    12961 tctgctgctc tttctcattg cgtggttttg caataccaga tatcagcagt tggtttcggt
    13021 tgtactcttt gaagtcatca aggccttcgc catcgatatt gtccagaata ataccctgaa
    13081 ttgccgggcg cattgggtct gttggaagca tagagctaag gacatttgtc agtacagaaa
    13141 ccgttgcatc acgtcgtgct gtgtagcttg gtccaacatc aaccgtcaca tcgtatcgac
    13201 cgacagaaag gtcatttaac gcaacaacag cccctgtttg cctgtcaaca acctgtgcgc
    13261 tcaggaccgc gatatcatca cttccatctt cgttaacgat gcgcacttca cgctctgaac
    13321 cgtacacttc acgcgccatt gacagccata cttcaccagc gcgtttaaga cttttcgcca
    13381 tattgtccag atagataaac gaagccatat ctgctctgtt catcaagttg ttaaccgttt
    13441 cctgagcaat attacttggc atctgctgca tggcctgact gccgcctgta acctcctgaa
    13501 tatcagcact ggtttgctgt agtaatgcag ccaatgcctg attcataacc gcaggctgtg
    13561 tatatcctgc cggggtagct ccagcgataa tgttgccaga tttatctctc acttcgcgca
    13621 acggcaagaa cgctgggcgt ttcttgttgc gagcctccca gtgcttctca agtccacgaa
    13681 tttgctccat gccaactata gggatctgac cggggtcttg cgctgcagta tcagccagca
    13741 ttgatacctg aaggttatac aaacgctgtg gatccattgc ttttgcaata tgtccttcga
    13801 cacgctcaat gtcatcaatg aaccagcgtt ttccataaac cgggatgagg gggatatgct
    13861 caccaggaat acgtcgaggt ttctcaagga aaccatcacc atccactacg gatacataca
    13921 cacgacggcg cttcactgag cgccttgcca cttcctgaaa tccagctatt gccagttcat
    13981 cttcaatatc ttcaacctga tcactgtcgt atgttgcaat ctctccagtg attggatgtc
    14041 gataactgat gacgccaaca gactctttac gaacttcgta atacttcgct atgtaaataa
    14101 catcttcatc aaaccagtca tattcccaac tggtcataga cgttacatcc agagaagcag
    14161 gaggtttctt tccgtattca gcctcatatt tttcaggtga caacgaatac atgcagaacg
    14221 cccacaacgc gtcagatttg tcgtacttct tagcgtcagg gtcaaaccac acagagcgcg
    14281 acgggtcgta tattggttca atagcaatac gctgacgatc gtccatgagg tcgtattcat
    14341 tgaccagcat cgacgtcaaa cggaagcaac cgaaaccacc agtagcagcg tcgtcaaatg
    14401 cattatcgca agcctcaccg ccatcggttt cttcgtagtc agcacggaac agaccattta
    14461 atttattggc taactcttcg cttgcctctc tgtcaccagg acgaaactta acagtgattc
    14521 tgttattgcg gtattctgca atgatgcggt taagttcagt tgctacctta ttgatttcaa
    14581 acttaggata cttctcgaac tgctcatcaa gcttagttcc agccgccgtt gctccttccc
    14641 attgacctcc tgggacacga gcaaacctcg tagcttcaat gcacttttcg cgcacttcct
    14701 gctgtggaga ataggcgcgg tcaaacctga gcatgatccg ctcatgtttt ttctctaatg
    14761 tctctgccat gtttaccaac cggaggatga gggaacgtat atttctgttt cttcgcggac
    14821 caatgccggg caatgcatac acatcatcag cgcatcagcc aggttaggag atgggatacc
    14881 gagcttctgc ttcatttcga ccttagtcat aagctccagc ttcccgttat tattgaattt
    14941 gcgctgaatc tgcgtcagtt ctgcaaacag cttctccagc atcttctcgc ctatcgcttc
    15001 tttgtcgaag ctcagcatgt cgtcggggtc tgcatactca ccgtggacaa ccgcccgata
    15061 tgtcagatac agcctgtcag ccagcgcgta atagaattgc gctcgcttat tgcggaatac
    15121 atcgccaata gtgcgaacgt tgtcgccctg tacgacttca tcagcccatg ctccggcctg
    15181 ataaggccca tcttcatcga atggcgattc gctgcccttg aacatcgtgg cggtaatttt
    15241 cttgccggag aatgcttccg ttgtctgtct gcgtaggccc gcaccgacgc catcaccgtc
    15301 ccacaggtaa tggtcagcgc cgtcttcaat cgccagcgaa gtagcccagt cagcaccctc
    15361 gttgatgtcc atcagcagac cttcggcaat gcgcttaact accgaaccgt gacgcgatgc
    15421 ataaccttta gcatctggcc ctgtatctga tgggtcatgc gcagaaacaa ccgcaccttt
    15481 cgctttccac ccgagtttcg tgtgcgcatc ggttgcggct tcaagccatt cacgtttgat
    15541 gattgccata tcacttgcgc ttaccggctc accaagccag atgtgacgat acagtgtcgg
    15601 atttctgcgt ttacactctt ccatctccag acggaggact tcaggaaaat gcgggttgtc
    15661 ggtgtagttc accgtcagca ggcaaatatc atcgggagga tttacgacga atcgctgata
    15721 ggtatcgtcg aggatgtttt tcgggttgaa acttacccat atttcagaga acggcttacg
    15781 gatggttgga atcaggatat cccacgattc cttcgttacc gcttccgctt cttccaccca
    15841 gcagatatca atgccttcga gcgatttaat cttcgtcggg ttgtttttga tgccgtagaa
    15901 catgaattca gcattcgttc cgagatgacg aatcattgaa cgctgaattt caaactcagc
    15961 cgaataccct tcacgctcga tggtgtcttc aagcaacctg attaccgaat cgctgatact
    16021 gttttgcagc tcacgagcgc aaagaatacg cacaggctgc cgacgcgccg cttcaacaag
    16081 aagcctcgca attgcccatg acttaccgct acctcgaccg cctttggcga ctttgtagcg
    16141 atgagcctca atgaacggtt caaagatagg attaatcgag gtcattttcc gaatagagtg
    16201 ctcatcggtg atgtttcaat ctggattgcg ccgccgtctt tgccgacaag ctcgttagtt
    16261 accttgtcgc catacttacg gggattcatt cgggccagcg cccatttgcg ggtatcaacg
    16321 cgaagtcttg cctttgccac ctcggcggca tcagggatta cgtcgtcagc aatttcgaat
    16381 atctcttcga aaatagagtc ggcccgagtc tctgttgcct tcgcgtactg gtcacgaaac
    16441 tcctgatgtt cagccagcca gcgaaaaact gatgttttgc tcggcattcc tgggcgttcg
    16501 caaactttgc gcagactctc accggaggaa agcaatgcgc aaatgtcatt agccacctcc
    16561 ggcatataat cagaggggcg accacctttc tttttctcag tcgccatatt gattatttcc
    16621 cttctgcttg cttatcccat tcatcgcgga atttggatgg gttgtcgaaa ccttgagttg
    16681 ccatgtttac gctccggtag tgaacaggtc taacgcttcc ttcgatttac gcaccgcttc
    16741 gattgtgcgg gtcgtgatat ctgaattagc gccacctgac tggaagtgaa ttttgaatag
    16801 ctcaagcttc agctcgtcag tgccaatgaa ctgaaatgct tcctccgcgg ctgcgttctg
    16861 gttcatgacc agtttgtaaa tctctaactg gaatttctgt tcttcagtca tgggaataat
    16921 ctctgccatt gttggctccg tttatccgtt aaaagggata tcagttaagt tatcccgtgt
    16981 agggtataag ccattatcaa agccactcag taaggaatgg cttttgtaat aactactgtt
    17041 cgcttagctt ctgcttcagc aagtaacctt cgagcatcca gattttgttt acagcattct
    17101 gccgggcaat cttccgacca atttctgcat caaaattttc ctgacttgca caggcactct
    17161 ctccggtgac ggtgaagccg ttgcgcagca ccaggacgca gaacgtcagc agagaaagtg
    17221 attcgtgcga ctggtagttt acctctccgc cagtatgttt cgcttttatg gctttgccaa
    17281 aggcaccatc ctctgctgtg aaatatgcct cctgagcaat aattccttcg atatggtctg
    17341 gcgtaactcg cggtgccgtt ttgcctttct caacgatttc tttttcgatt tgctggtcgt
    17401 tcataattat gaccctgtag agtggttgct tgattaggat gtctttccat cagttcgcca
    17461 ccacaaagaa tcttttttgc cataaggctg gaggttcatc tttcagtggc tgccagtgtt
    17521 atttccccac ttactggctt gggttgtttc gcggtactgc cgtaactggt ggtgcacaga
    17581 tttagttaaa tctgttctcg cctgaactat cttttacata cccggattgt ggggatgtaa
    17641 atcacggttt cattatcaag cccacccgta gatgggcttt ggaatggtca ctttggcagt
    17701 ccggggatcg atatttgcgc ctgctgctca agccttttga ttcttgctat gagttgcggt
    17761 tttttgatcc tgccccagcg gttcagcaag cgtcctgaca tactggcaac atccttttcc
    17821 ttcatgaact ccagcattaa ctcgttgtgc tctctttggt atgagtgagc catctccatc
    17881 agcctgtcac gcatccaatt aaatgctttg ataaacgcct ctttgatggc ggcagctttt
    17941 ttgccggtaa acgacatgat gatgtacatc gcaccgtctt tggaaatttc atattcaaca
    18001 tactgattac ccttgtgttc ataggtaacc cgcgaaaagt tgctggttag aaattcatcc
    18061 gaacagtcta gcttttcgat tttctgaatg atgtggtgat gctgcttgtc gaagtaagct
    18121 gctaccttgc gggaggttgt gatcacgcga tcaccagaaa caaccaccat gtcccggaaa
    18181 tcgagattag ccaattgatg attcatagcg tctttacctt ttagaaagtg agcctgtctc
    18241 acagaaaagc cgccccgaga tggtcgccac catatacggc agttctcagg ctcaactttc
    18301 tgaaaggctc gggtgatgta atatgcgcgt gagatgcgct gtgaaattca gatgtaaaaa
    18361 aagccccgca tcgcgaggct cattaaatgg actttgtgat ttgcaaaaaa attatttcag
    18421 gcactgagtc ctgatgtact cctgcaggta gttaacctgc gcggttatcc tgtcgattcc
    18481 acttcggaga cggtaataat tgagttcagc atctgctgta agtcttgggc tttctccatc
    18541 gcccatgctg ctggctccgg tcgttgactt tgcacaggtg gcggcgactt gcaggcgctt
    18601 acgaccagca gaaacatcag cacgaaggct ttcgatagta gcgttagcat cagcaagctc
    18661 ctttgtgtat ctggcgtcga gttctgctac atcacgttga cgcttctgca tatcagcgat
    18721 gatggatgtg gctttatcgc gctgctcttt gtaggtcatg gcgttatcac ggtaatgatt
    18781 aacagcccat gacaggcaga cgatgatgca gataaccaga gcggagataa tcgcggtgac
    18841 tctgctcata cctcaatctc tctgaccgtt ccgcccgctt ctttgaattt tgcaatcagg
    18901 ctgtcagcct tatgctcgaa ctgaccataa ccagcgcccg gcagtgaagc ccagatattg
    18961 ctgcaacggt cgattgcctg acgaatatca ccgcgatcaa tcatcggcaa agcgccacgc
    19021 tctttaatct gctgcagcgc aacagcgtcc tggcttttgg gagagaagtc tttcaggcca
    19081 agctgcttac gataggcatc ccaccaacgg gaaagaagct ggtaacgtcc ggcggctgtt
    19141 gatttgagtt ttgggtttag cgtgacaagt ttgcgaggat gatcggagta atcagtgaat
    19201 agctctccgc caacaatgac gtcataacca tgatttctgg ttttctgccg tccattatct
    19261 gttccctctg accacgccag catatcgagg aacgccttgc gttgattatt gatttccacc
    19321 atcttctact ccggcttttt tagcagcgaa gcgtttgata agcgaaccaa tcgagtcagt
    19381 accgatgtag ccgatgaaca cgctcgttat ataagcgaga ttgctactta gtccggcgaa
    19441 gtcgagaagg tcacgaatga accaggcgat aatggcgcac atcgttgcgt cgattactgt
    19501 ttttgtaaac gcaccgccat tatatctgcc gcgaaggtac gccattgcaa acgcaaggat
    19561 tgccccgatg ccttgttcct ttgccgcgag aatggcggct aacaggtcat gtttttctgg
    19621 catcttcatg tcttaccccc agaaggggat ctgttcaaat taggaattat ggatatggtc
    19681 gcttgaacaa atccgggtta cggttgattt gtaacgggtt tgttcgtgac cgcattcatg
    19741 agcaaatcag gcgtggattg cgccaacaat acatgccgct catatcacga agcccagcca
    19801 ttgatgctgg gttttctttt ttaaagcgta ctagacaacc gtatccacag agtgtcagca
    19861 atgagttggt taggtctggt tcttggtgga agtacgcttt aaaaaatggg ctgatggtgt
    19921 agcccaaaat actgagtgaa tggtaaggat gaacaacggt tttgctctgg gtggatttgg
    19981 ctgtggtggc cggcgctgat ctccggcttg tatacaggca ccttgttctt ccgaagctct
    20041 cctgcgcgca tcagcctgcg cattcaccac accggaaaga gcactcagtt gtaccggcca
    20101 gttgtgccac taagaaatgc tttcgcagac cgttaagctc tttgccagtt ctttaatgct
    20161 cttacctgtt gtgtgcccat tattaatcac accgggccag tgcgccgaat ttgtttacaa
    20221 ggagtcggaa gaccttgctg acttacaggc tattacgccg ccatcagaac aacatcatcg
    20281 tttgcattta tctttgtggt cagtttctaa aaaaccgcaa agtcgccaac tctgacgaaa
    20341 actatcgttg tgctgccaca acgataagag cactcggtgc atttaagcca agccccataa
    20401 gggagaatgc tcttacctgt tacacagata taaaaaatcc cgaaaccgtt atgcaggctc
    20461 taactattac ctgcgaactg tttcgggatt gcattttgca gacctctcag cctgcgatgg
    20521 ttggagttcc agacgatacg tcgaagtgac caactgggcg gaatcggtag taagcgccgc
    20581 ctctttttat ctcaatacca caacgagcga attaacccat cgttgggtca aatttaccca
    20641 actttattca ataagtcaat atcatgccgt taatatgttg ccatccgtgg caatcatgct
    20701 gctaacgtgt gaccgcgttc aaaatgttgt ctgcgattga ctcttccttg tggcattgca
    20761 ccaccagagc gtcatacagc ggcttaacag tgcgtgacca ggtgggttga gtaaggtttg
    20821 ggattagcat cgttacagcg cgatatgcgg cacttgctgg catccttgaa tagccgacgc
    20881 ctttgcatct tccgcactct ttctcaacaa ctctccccca ctgctctgtt tttgctatat
    20941 caaccgcacg gcctgtaccg tgacaatctc tgcatcttgc gcccggcgtc gcggcactac
    21001 ggcaataatc cgcataagcg aatgttgcga gcacttgcag tacctttgcc ttagtatttc
    21061 cttcaagctt tgccacgcca cggtatttcc ccgatacctt gtgtgcaaat tgcatcagat
    21121 agttgatagc cttttgtttg tcgttctggc tgagttcatg cttaccgcag aatgcagcca
    21181 ttccgaatcc ggcttgtgat tgcgccatcc ccatagcagc catcacatca gtaccggaaa
    21241 gagagtcaga agccgtagcc cgtggtgagt cgctcatcat cgggcttttt ggcgaatgaa
    21301 atttagctac gctttcgagt ctcatgcgcc ttctccctgt acatgaatca atgtgaggtt
    21361 tccgcagaac actgcgccgg tatcgatata catctggttg gcaaacttga gtggtttcac
    21421 tgctggcgta tgaccaaaga tgaacatgtc cgctcctttg atttctttca cgatcccgtc
    21481 ttgtgagttg ctgattcgtt cgcggttcca aattaactgc tgatgatcaa ctggctttcc
    21541 aaactcgtat tcgtcacaag gataatcggc gtggcagatg acatattttt tatctttgct
    21601 caccagttcg atgattaacg gaagttcacc tgctttatgg gcaagagctt tagccagaat
    21661 ttctttgtcg taatcgagat taaagaacca gccaccgcca ttaagcagcc agtgattgac
    21721 gtttccacgc tctgataagc catcaatcat catttgctca tggtttccac gtacagctct
    21781 gaaccagggg aatgtgatta attccagaca ttcgacgttc tctgtaccgc gatcgaccaa
    21841 atcgccaacc gagataagca ggtctttttt ggtgtcgaat cctatcgtct ccagtttttt
    21901 catcaggttc gtgtagcatc cgtgcagatc gccaactacc caaatatttc ggtatttgct
    21961 gccatcaatt ctttcgtaat agcgcatctc tttcactcca tccgcgatga accataagaa
    22021 cgtcgttgac gatggcgtgc attttcccgt ctttatcatc aacgtatttt cttaccgtgc
    22081 cgcgactaca tttcagtctg cgtgctactt ctgtctggtt tccgtatgct tcaacgagca
    22141 tgtctggaat ggtttttact gagaacgtca tgcggcctca cttctgctat ttcgcaggtc
    22201 tttgagtttc tgttggtact ctgccttgat cgccttgcac tcttcgatag tccagcgatg
    22261 gcggctatgg tttgattcga tttcgtctac tgcttcctgc cctatgcggt taatcagttc
    22321 gacgcgatac ggaacgagat ttccgctttt gtgctggttg cacaccacgc attgcttgtg
    22381 aatattgcgt tcatcaaatc ggagttgagg tgccgcagca gttgtccggt aatgtccggc
    22441 atcccactga gcagacgtga gcgttccgca cgagatacat ggtaagtcgc ggtctctttc
    22501 tctgatgaag gcgtttacag cttgttgggc ttgtttaatc cagtaactgc ggggctttaa
    22561 ggcgagtttt cgaatcttaa gtttatcttt ctgtttctgc tcctctcgtc gtcgtttctt
    22621 ctctgctgct ttttccgctt tttcgcgttc tttacttcgt cgttcgagtg ctaattgagt
    22681 tccgtgttcc gggcagcacc accactgatt tgagaatgcc gggtgaaacc attccttgca
    22741 tattttgcat ttccttctcg ctggtttagc catcgtattc ttcctcgtac attgagctat
    22801 tcggatcgct catcagttct gcacagcagt
//
LOCUS       DAZFRJ010000060        22549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000060 DAZFRJ010000000
VERSION     DAZFRJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.060"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..354
                     /locus_tag="ACHKUN_002153"
     CDS             <1..354
                     /locus_tag="ACHKUN_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO6660404.1"
                     /translation="NGTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEP
                     LSAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMA
                     QMELVMKAAGFKEVK"
     gene            complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHKUN_002154"
     CDS             complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHKUN_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO6660405.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHKUN_002155"
                     /gene_synonym="yehT"
     CDS             complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHKUN_002155"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO6660406.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHKUN_002156"
     CDS             complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHKUN_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO6660407.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHKUN_002157"
     CDS             3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHKUN_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO6660408.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4325..4432
                     /gene="yohO"
                     /locus_tag="ACHKUN_002158"
     CDS             4325..4432
                     /gene="yohO"
                     /locus_tag="ACHKUN_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO6660409.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHKUN_002159"
     CDS             complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHKUN_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO6660410.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHKUN_002160"
     CDS             complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHKUN_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO6660411.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHKUN_002161"
     CDS             complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHKUN_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO6660412.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHKUN_002162"
     CDS             complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHKUN_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO6660413.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHKUN_002163"
     CDS             complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHKUN_002163"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO6660414.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10853..12568
                     /gene="dld"
                     /locus_tag="ACHKUN_002164"
     CDS             10853..12568
                     /gene="dld"
                     /locus_tag="ACHKUN_002164"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6660415.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHKUN_002165"
     CDS             complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHKUN_002165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO6660416.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHKUN_002166"
     CDS             complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHKUN_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO6660417.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14469..15047
                     /gene="yohD"
                     /locus_tag="ACHKUN_002167"
     CDS             14469..15047
                     /gene="yohD"
                     /locus_tag="ACHKUN_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6660418.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHKUN_002168"
     CDS             complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHKUN_002168"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6660419.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHKUN_002169"
     CDS             complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHKUN_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO6660420.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17651..17734
                     /gene="yohP"
                     /locus_tag="ACHKUN_002170"
     CDS             17651..17734
                     /gene="yohP"
                     /locus_tag="ACHKUN_002170"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO6660421.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18122..19072)
                     /gene="dusC"
                     /locus_tag="ACHKUN_002171"
     CDS             complement(18122..19072)
                     /gene="dusC"
                     /locus_tag="ACHKUN_002171"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO6660422.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19311..19709
                     /gene="yohJ"
                     /locus_tag="ACHKUN_002172"
     CDS             19311..19709
                     /gene="yohJ"
                     /locus_tag="ACHKUN_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6660423.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19706..20401
                     /gene="yohK"
                     /locus_tag="ACHKUN_002173"
     CDS             19706..20401
                     /gene="yohK"
                     /locus_tag="ACHKUN_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO6660424.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20531..21415
                     /gene="cdd"
                     /locus_tag="ACHKUN_002174"
     CDS             20531..21415
                     /gene="cdd"
                     /locus_tag="ACHKUN_002174"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO6660425.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21565..22284
                     /gene="sanA"
                     /locus_tag="ACHKUN_002175"
     CDS             21565..22284
                     /gene="sanA"
                     /locus_tag="ACHKUN_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO6660426.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22287..22526
                     /gene="yeiS"
                     /locus_tag="ACHKUN_002176"
     CDS             22287..22526
                     /gene="yeiS"
                     /locus_tag="ACHKUN_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO6660427.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
BASE COUNT         5384 a         6093 c         5656 g         5416 t
ORIGIN      
        1 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
       61 tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga cgctgccaag
      121 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
      181 tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa
      241 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
      301 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatcggtt
      361 ggcggtcatg ctctaaacat gaccgccaat tttttagcct tttttcacat gctggcgcgc
      421 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
      481 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
      541 gaggatagcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac
      601 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
      661 tttttcataa atcaggccat tgaggaatga cgacaaaaaa atgtccggac aacgctgaaa
      721 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
      781 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
      841 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
      901 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
      961 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
     1021 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
     1081 tttccagggt acgtaatgtc aattcggtaa agccctcttt cccttcgtgg ctggtaacgt
     1141 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
     1201 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
     1261 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
     1321 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt
     1381 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc cccaccattt
     1441 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
     1501 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
     1561 gctcctgcaa aaatacgcgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
     1621 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
     1681 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
     1741 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
     1801 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
     1861 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
     1921 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
     1981 gaagcgcgcc ttttcaattt gcagataagc gttcacatgt tcaatttcgt cggcgagagt
     2041 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
     2101 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
     2161 caaaaaatga ggattcacct gggcgtgaag cagtttgatt tctgactggg tgagcatcgc
     2221 tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa agcagttgcg caatcccctc
     2281 gcccagcgtg cggtttattg aactgaataa acggtttttg gcttcataca atttgatggt
     2341 gcccatcacg cgctgatttt cgccacgcaa cggaattacc agcgtcgacc ccagtttgca
     2401 ttgcggatgc aaagagcaac gataaggtac ttcgttgcca tcagcgtaga ccacttcacc
     2461 ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt ttgccgggta aatggtggtc
     2521 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
     2581 atccagctcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
     2641 tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagcagaagt
     2701 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
     2761 ggtattggtg accatcattg gtgcagcaat attactcacc agacgcaccg catcttcata
     2821 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
     2881 accggcggta atggggttaa agactttatc agtgcgcccg cgacggatca ggatgctgtg
     2941 taccaggccg ccgagcaatc cttcaacgat ggttgagatc atgcaactta gcgcggtcat
     3001 gccccccatc gaatatcgat gtaagccgcc ggtcagacca accagcccac cgacgaccgg
     3061 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
     3121 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
     3181 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
     3241 cattaaccac gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
     3301 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
     3361 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
     3421 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
     3481 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
     3541 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt
     3601 ggcagaggcg ttacgggttg ctgaaaccgc aacggacaga cggcggtcat cgactgttca
     3661 acgatgccga tattgaccgt atccgcgaga tcaaacgctg gatcgacaac ggcgtgcagg
     3721 tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga tgtgcagaac ggctggcgcg
     3781 atcagcaaga aacattactg acctacctgc aaagcggcaa tctgcatagc ctgcgaacgt
     3841 ggatcaaaga gcgcggtcag gattaccccg cccagacact caccacacat ctgtttattc
     3901 ctctgcgccg acgacttcaa tgccaacaac cgactctcca ggcgctgctg gcgatcctcg
     3961 acggcgtact gatcaactac atcgccattt gtctggcttc ggcacgtaaa aaacagggta
     4021 aagatgcgct ggtggttggc tggaatattc aggataccac ccgtctgtgg ctggagggct
     4081 ggattgccag tcaacaagga tggcgcattg atgtcctcgc ccactcgctc aatcaactac
     4141 gccctgaact attcgaaggt cgtacattgc tggtgtggtg cggtgaaaat cgaacctccg
     4201 cccaacagca gcaactcacc agttggcaag aacaaggcca tgatattttc ccactcggca
     4261 tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc tttcgttaac ataaggagtg
     4321 cattatgcgc atagctaaaa ttggggtcat cgccctgttc ctgtttatgg cgttcggcgg
     4381 aattggtggc gtcatgctcg caggttatac ctttattttg cgtgctggct aagcgcctgc
     4441 accagccttt caaacaggcg gtctgcgatg atcgccgcca gtgccaccag taacgccccc
     4501 tggatcacat acgcggtatt aaatccgcta agcccgatga tgatgggcgt acccagcgtg
     4561 ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt tgataatcac cgaagttcgc
     4621 acgcccgcca gaatcaccgg agccgccagc ggtagctcga ccttacgcag tcgctgacca
     4681 cgactcattc ccataccttt cgcaacttct gtcacgctgg catcgatcgc tcccagcccg
     4741 gcaagtgtcg cctgcaggac gggcagcaca ccgtaaagga tcaaggcgat aatcgctggt
     4801 tgcagaccaa agccgatcac cggaacggca atcgccagca ctgcgacagg cggaaaagtc
     4861 tgtccaacgg cggcaatagt ttccaccagt gggcgaaatt ccgcgcccca cgggcgagtg
     4921 acagcaattc cggcaccagt gccaatgatc accgcaaaca aactcgaaat tcccaccagc
     4981 cagaaatgag ccagtgccag agctgcaaaa ctttcttgct gataaacggg tcgtggcagt
     5041 tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca gccagaaaat cagtgccaca
     5101 aacaaagcaa tgagccagaa cagcggatcg cgcaacatct tcatacgctt acgcctccac
     5161 cagcagatcc tgaaaatgca gcgtaccgca aggctggccc tgcgtgttca ccaccggcag
     5221 cacctcgcaa ccccgcgcga caaacaggga gagcgcatcg cgtagcgtca tctcttctgc
     5281 cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa tccgccacac tacgtaacga
     5341 aagcagacgc acacccagtt cgctacgtcc aaaaaactgg cggacaaaat cattcgccgg
     5401 acgagtcagc atcgtcagcg gattgccctg ctgcaccact tcaccgtgat ccatcaatac
     5461 cagatgttca gccagccgta gcgcctcatc aatatcatga gtgaccagca caatagtgcg
     5521 ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac gcgccgcgcg ttaccgggtc
     5581 cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga tcggcagcca gtgcgcgcgc
     5641 cactcccaca cgttgctgct gaccaccgga aagctgatgc ggataacgct cacgcaaatt
     5701 tgactccagc cccagtagcg tcattaattc gtcgatacga tcgtcaatcc gtgcccgcga
     5761 ccatttttgt aattgcggca cggtagcgat gttttgcgcc acgctccagt gggggaatag
     5821 gccaatagat tgaatggcat agcccatccg gcggcgcaac tccagtactg gcagcgagcg
     5881 aatttcttct ccggcaaagc ggatctctcc gctgtcatgt tccaccaggc ggttaatcat
     5941 tttcaaggtg gtggatttgc cggagccaga tgtgccaatc agcaccgaaa aactcccttc
     6001 ctgaaaattg agattaagat cgttaacggc tttttgtgcg ccaaacagtt tgctgacatg
     6061 gctaaattca atcattacgt ttcaccttca gcagtgcgat aagcaaatcg aacagggcat
     6121 cgatcagcac cgccagaaca attaccggga tcacccccag caacactaaa tcaatggcgc
     6181 tgcttagcag cccctggaaa accagcgcac caaaaccgcc tgcgccgatt aacgccgcaa
     6241 tcaccgccat acctacagtt tgcaccatca ccacccgcag gctgcgcaga aataccggta
     6301 acgccagtgg taactgaaca tgcaggaatc gctgcgcccc gctcatgccc atcgctctgg
     6361 cgctctccag cacatcgcgc gggatctggt tcaaaccgac taccacgccg cgcaccagcg
     6421 gcagcaaggc atagagcacc agcgcaatca gtgcgggtgt cattccggtt cctgctatgc
     6481 cgagcttccc cagccacgga aaggccgtca ccagcgcggc aagcggcgca atcaacaggc
     6541 caaagagcgc caccgaaggc acggtctgaa tgacattgag cagagaaaaa attgccccct
     6601 gccgagcagt ggaaaagtag caccagatgc ccaacggcac accaatcact aacgcaggca
     6661 gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc gtcgtcaaac acatcctgac
     6721 ggttggcgta ttctttcatt agtgagagat cgttaagcgt gccggagtac agcaaccaca
     6781 gcggaataat ggcaatctgc atatgcaaca accagcgcca cagcggatgc gtggagattc
     6841 ggcggatggc atcgctacag gccagcaatg ccagcgccgc agccagccag aaaccactgc
     6901 cgaggctggt acgcgccagc gcactgccat tttgcgccag ttgtgtcgcc gcctttcccg
     6961 cgccccacac cagcaatacg aagacgaatt gcgccagaat gagtgcacaa atgctgcctt
     7021 ttttaccggg aataaaacag gccgtcagcc aggcgcaacc aacgcccacc agcatccaga
     7081 tcgtttgcgg ccacagttgc cagagatgac gcccttcacc cgagactaaa cgattaggcg
     7141 cgtagctgat aaacggcagc gctgctgcga ttgccgtcag caacagcagc agcgccagaa
     7201 caggattaat acgtagataa gtcacgggaa attacttcgc ccaccctttt tgtttcaggt
     7261 agtcggcagc cacttttttg gcatccagtc cttccactgc aatgctggca ttcagttgct
     7321 gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag ccactgtgcc atttgcggat
     7381 actccttcaa caccgattca cgcaccactg gtgcaggcgc atagataggt tgcacgcctt
     7441 gcggatcgct taaggtttgc agccccagcg ccgctaccga gccgtcagtg ccgtaagcca
     7501 ttgcagcatt aacgccagag gtttgctggg cagcggcttt gatcgtcacc gccgtgtcgc
     7561 caccagccag tgacagcaac tgatcctgac cgagcttaaa gccataggct ttttcaaacg
     7621 cgggtaacgc atcggcgcgt tcgataaact ctgccgaggc tgccagtttg aaggtgccgc
     7681 cctcttgcag ataacgactc aggtcggcaa gcgaagtgag tttatttttc tctgccacat
     7741 cctgacgcac ggcgatagtc caggtgttat ttgcaggcgc gggcgtcagc cagattaact
     7801 tgttgtgctc cgaatcgagc tttttgactt tctcgtaacc ttgctgcgcg tttttccacg
     7861 ctgcatcgtt ttcatcttta aagaagaaag cgccattgcc ggtatattcc ggatagatat
     7921 ccagttcacc ggaagtaatc gccccgcgca ccacaggagt cgttccgagt tgcactttat
     7981 tcaccgtcgg tacgccgtgg ctttccagca cctgcaaaat gatattgccg agtagcgcac
     8041 cttcggtatc gatttttgaa ccgactttaa cgggggaagc cgcctgtagc ggcaggctca
     8101 cggctgccaa cataaccagt gaacctgccc agagctttaa gagtggcatg atgctttcct
     8161 cattctttta ctgttgtttt cagcgaatta agagaaaagc atagttgata atggcggggt
     8221 tagcttgagc ggaaggattc agttgcagaa tcagataaat acttaagagg catattcggt
     8281 ccggcgtttc ctgccggatg cggcgcgagc gccttatccg gcctacaaag ggcgcaaacg
     8341 tcgcgccctt actaaagcat tacagcaact caaactcgcc tttcttaacg cgtgcggaat
     8401 cagtgccgat aaatacattg aacttgccag gctcggcgtc atatttcatc tgttgattcc
     8461 agaacttcag cgcctcaata tcgatcggga agctgacagt ctgagtttcg cccggtttca
     8521 gggtgatttt ctcaaagcct ttcagctgtt tcacagggcg actcatggaa gccgtcacat
     8581 cctgcaagta catctgcact accgtggccc cctcgcgctt accggtgttc gtcacctgca
     8641 cgctggcagt cactttgccg tcacgcttca tggtcggcgc agaaagtttc acatcagaga
     8701 cggtgaaagt ggtgtagctc agcccatagc cgaacggata caacgccccg ttagcttcat
     8761 caaaataacg cgaagtgtat ttgttcggct tgtcggcatt atacgggcga ccggtattca
     8821 gatggctgta gtacaccggg atctgcccga cagaacgcgg gaaggacatt ggcagcttgc
     8881 cggacgggtt gtaatcgcca aacaatacat cggcaattgc attaccgcct tcagtccccg
     8941 caaaccaggt ttccagaatc gcatcagcct gctgatcttc tttcaccagc gccagcggac
     9001 gcccgttcat cagcaccagc accagcggtt taccggtggc tttcagcgcc gcaatcaagt
     9061 cacgttggct ttgcggaata gtgatatcgg tccggctgga ggcttcgtgc gccatcccct
     9121 gtgcttcacc gactacagcc accaccacat cagattgttt cgccgtctgc accgcttcat
     9181 caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc ttcttcatac tgattcagga
     9241 aatcgataat gcctttgtca ctggtaacgt tcgccccttt ggcatacagc actttaccgt
     9301 tttcaccgac ggaattttta atcccggtca gtacggtcac ggattgatcg gcaacaccgg
     9361 ctgcggacca gctgcccatc acgtcacgtt tactgtccgc cagtggccca accaccgcaa
     9421 tggtggccga ttttttcagc ggtaacgttt cgagacggtt tttcagcaac accaggcttt
     9481 cgcgcgccac ttcacgcgct tctttacggt gcaggcggct ttcggcattg gtatccaccg
     9541 ggtcagactc tttcggcccc aaatggctgt atgggtcgtt aaacaatccc atatcatatt
     9601 taacgttcag tacatggcgg gcagcatcgt ccagctcttc catcgtcact ttgccggatt
     9661 tgatcaaccc aggcagatac ttcgagtaat actcgtcgct catactcatg ttgattccgg
     9721 atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc cgtgccatgt ttaatcagct
     9781 ctttgattgc accgtgatcg gaaacggtga tgcctttaaa gccccactgg tcgcgcagaa
     9841 catctttcag cagccaggaa tcggaggtgg ctggcgtgcc gttcagcgag ttcagcgcca
     9901 ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta cggcggcata taatcattaa
     9961 acaggcgctg cggactcata tcgacggtgt tgtactcttt accgccttct accgcgccgt
    10021 atgcggcaaa gtgtttgacg ctggtcatca ccgagtagcg atctgccggg cttttaccct
    10081 gcatcgcttc caccatggtt ttacccattg ttgaggtgag atacgtatct tcgccaaaac
    10141 cttcggaagc acgtccccag cgcggatcgc gcgagacatc gaccatcggt gcccaggtca
    10201 tattcaggcc atcatctgcc gcttcataag cagagacacg tccgaccgtt ttcactgcat
    10261 cgaggttaaa agacgaggcc agaccgaggc taatcgggaa caccgtgcgc tgaccgtgca
    10321 gcacgtcgta agcaaagaaa agaggaattt tcaggcggct taattccatc acctgatcct
    10381 gcatggcgcg gatatcctga cgggttacgg tgttgaaaat cgccccaacc tgaccgtctt
    10441 tgatcatctc gcggatcgcc tctttcgggt tatccgggcc gacgctgatt aagcgcagct
    10501 gaccaatttt ctcatcaact gtcattttct taagcagttc ggtgacgaac gcatcccgcg
    10561 cttcgggcgt taatggatgg ttgccgaata aatcatccgc cagtgctggc tgcagggcca
    10621 gactcaccgc gattcctact gaacatagcc atttcatatg gatttttctt cctctgttgc
    10681 ccgacgaggc agcgaaaatg gtgcaaaaac cgtagtttgc cataagcatg atggagagag
    10741 aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat tcactcgctg aattgtcata
    10801 caaggcgcta ttctagtttg tgatattttt tcgccaccac aaggagtgga aaatgtcttc
    10861 catgacaaca actgataata aagccttttt gaatgaactt gcccgtctgg tcggtcattc
    10921 acacctgctc accgatcccg caaaaacggc ccgctatcgc aagggcttcc gttctggtca
    10981 gggcgacgcg ctggctgtcg ttttccctgg ctcactactg gaattgtggc gggtgctgaa
    11041 agcctgtgtc accgccgaca aaattattct gatgcaggct gccaatacag gcctgaccga
    11101 aggatcgacg ccaaacggta acgactatga tcgcgatatc gtgatcatca gcaccctgcg
    11161 tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg ctggcctatc cgggcaccac
    11221 gctctattcg ctggaaaaag ccctcaaacc gctgggacgc gaaccgcact cggtgattgg
    11281 atcgtcgtgt ataggcgcat cggttatcgg cggtatttgt aacaactccg gcggctcgct
    11341 ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc gcgcgtataa atgaagacgg
    11401 caaactgacg ctggtgaacc atctggggat tgatctgggc gaaacgccgg agcagatcct
    11461 tagcaagctg gatgatgatc gcatcaaaga tgacgatgtg cgtcacgatg gtcgtcacgc
    11521 ccacgattat gactatgtcc accgcgttcg tgatattgaa gccgacacgc ccgcacgtta
    11581 taacgccgat ccggatcggt tatttgaatc ttctggttgc gccgggaagc tggccgtctt
    11641 tgcggtacgt ctagatacct tcgaagcgga aaaaaatcag caggtgtttt atatcggcac
    11701 caaccagccg gaagtgctga ccgaaatccg ccgtcatatt ctggctaact tcgaaaatct
    11761 gccggttgcc ggggaatata tgcaccggga tatctacgat attgcggaaa aatacggcaa
    11821 agacaccttc ctgatgattg ataagttagg caccgacaag atgccgttct tctttaatct
    11881 caagggacgc accgatgcga tgctggagaa agtgaaattc ttccgtccgc attttactga
    11941 ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat ttaccgccgc gcatgaaaaa
    12001 ctggcgcgat aaatacgagc atcatctgct gttaaaaatg gcgggcgatg gcgtgggcga
    12061 agccaaatcg tggttggtgg attatttcaa acaggccgaa ggcgatttct ttgtctgtac
    12121 gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc gctgcgggcg cagcaattcg
    12181 ttatcaggcg gtgcattccg atgaagtcga agacattctg gcgttggata tcgctctgcg
    12241 gcgtaacgac accgagtggt atgagcattt accgccggag atcgacagcc agctggtgca
    12301 caagctctat tacggccatt ttatgtgcta tgtcttccat caggattaca tagtgaaaaa
    12361 aggcgtggat gtgcatgcgt taaaagaaca gatgctggaa ctgctacagc agcgcggcgc
    12421 gcaataccct gccgagcata acgtcggtca tttgtataaa gcaccggaga cgttgcagaa
    12481 gttctatcgc gagaacgatc cgaccaacag catgaatccg gggatcggta aaaccagtaa
    12541 acggaaaaac tggcaggaag tggagtaaaa attacggatg gcagagtatc gccatccgaa
    12601 ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc tttttgtttt
    12661 ttatagctca acgctgctgc cggcacaggc atcactttac cggtttcaat ccaggtacgc
    12721 aggcggctgg catcggcaaa atgggtatat ttgccaaacg cgtccatcac taccagcgcc
    12781 accggtttat tattgataac cgtacgcatc accagacaat ggcccgccgc attggtaaag
    12841 ccggttttgg ttaactgaat attccagtta tcgcgataca ccagatgatt agtattgcgg
    12901 aacggcagcg tatacgtcgg gttagagaag gttgccatat cttcccgggt agtacttaac
    12961 tgcccgatca acggatactg tttgctggca atgagcagtt tggttaagtc acgggcagtt
    13021 gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc gcgtgttgtt cattccgagc
    13081 gatttcgctt tcgcattcat tgccttaata aaggctttgt aaccaccggg ataatggtgc
    13141 gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag catatcttta
    13201 cggctgattt cgctattcag tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg
    13261 ctgatatcca cttttagttt ttcatccagc ggcagtcgtg catccagcac aaccatcgcg
    13321 gtcattaatt tgctgataga cgcaatcgga cgcaccagat ccgggtggtt cgaatagatc
    13381 actttgttgg tattcagatc aacaatcatc gcgctaccgg aggcaatttc cggttgtgaa
    13441 gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg ccaaaggcac agccagcatc
    13501 agggccaggc taaataaaga aactcgaaat ttcggcatga tgagcattca gatagtggtt
    13561 cacgcgcacg ggttgcgcac cgccggagta aggatttact gaggctagcg acgccatcat
    13621 aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg aaatgctgca ttgctgacat
    13681 ttacgccagc aatgcaatgt caaagaactt tctagaacag gcgatacccg tagccccata
    13741 gtataacggt tagggcgagc agcacttcca gtaccagcac gccaatcgcc agcgtcgaac
    13801 tggagaagct aaggccttcc tctttgttga tattcaggaa gctcggaata ccaaggtaaa
    13861 gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag cgcacacaac cagaccagcg
    13921 gataaagcgc caccagaccg cttaaaaaca gcggagttgc aacgtagccc gcgaagacca
    13981 tgcagtgcgc aagtgacgga cgctgcgggt aattgcgcgc catccaccag atgacccgcc
    14041 ccatcaccgc aaccccagcg agcataacgc cataaaacag gacagccagc gccagtccgg
    14101 taaaccagga caacttcagg atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc
    14161 caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt
    14221 gagaaatcgt ttcgttttcg cgattaatca cctgcatttc acgatcggga tgggaaaaca
    14281 gtccccagac atggctcata ccgccccctt gttgtgagtt catgaacctg acagttcaag
    14341 tataagtcag cttgtgatta ttttttgttc gctcatgtaa tttgcaccgt ctgatgtacg
    14401 tcctttttca gcgtgaatga tttccagggt gtatgcttac aggcaaccaa agggagacag
    14461 actggcctat ggatctcaat acactcatct cacaatatgg ttatgccgcg ctggtgatcg
    14521 gtagcctggc ggaaggtgaa accgtgactt tgctgggagg cgttgcggcg catcaggggc
    14581 tattaaagtt cccgctggtg gtactttctg tggcgcttgg cggcatgatt ggcgaccagg
    14641 tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt acgccgtttc tcgaaacatc
    14701 aggataaaat tgagcgggcg cagaaactta tccaacgcca tccgtatctg tttgtcattg
    14761 gtacgcgctt tatgtatggc tttcgggtga ttggcccgac gctgattggt gccagccagc
    14821 tgccgccgaa aatctttctg ccgcttaata ttctcggcgc atttgcctgg gcgttgattt
    14881 ttaccactat cggttacgct ggtggtcagg tgattgcgcc gtggttgcac aatctcgatc
    14941 agcatttgaa gcactgggtc tggttgattc tggtcgtggt tctggtggta ggcgtgcgct
    15001 ggtggctgaa acgacgcggg aagaaaaagc cggataatca ggcgtaaaac cattgccctg
    15061 gataaggcgt tcgcgccgca tccgacatcc ggtgcaccat gcctgatgcg gcgcttgtgc
    15121 gtcttatcat gcctaccaaa attttaccca ttgaaatatc cacacacctc actgcgctat
    15181 tctgggttga actgcggatt cgccaacata aagccgccat ccactatcaa cgactgcccg
    15241 gtggtgtaat ttgcgccctc cgaacaaagc cacgccacca ggctggcaat ctcatacgtt
    15301 gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg gcttcacgtc gctatcatcc
    15361 atgccgttca ttggcgtagc gatcgcccca ggtgcgactg cgttcaccaa aatcttatgc
    15421 ctgaccaact ccagcgccat tgctttggtt aacccgccga gcgcatgttt agcggctgtg
    15481 taggcgctgg catccggcag cggcgtatgt tcatgtaccg acgtaatgtt gatgatgcga
    15541 ccaccctgcc cttgtttcac catctggcgg gccgcaattt gtgagcataa aaatgcacca
    15601 tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag ccatatcaag aaacggcgct
    15661 ttggtcattg cgcccgcatt attcaccagc acatcaatac gccccagccg ttgaatgagt
    15721 ttctccagcg cctgtgcacc ttctggcagt ttaccgagat ccagctgcac aatctccgca
    15781 cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg ccccttcttc atctgagtgc
    15841 caggtaatgc caatatcaaa cccctgctgc gccagtaata acgcgcactc tttgccgatc
    15901 cccgaatcgg aggcggtaat aatcgcaacc tgtgccatcg agttctccac ttaacgctga
    15961 ataaacgtta agtatagaag gcgcatatca tcagcgtttg taacccccgc ccaacgcacc
    16021 agtgagttga atggaagcat ccagccactg cccttgcaat aacaggccat tggcccgctc
    16081 acgcagcgcg gggattctgg cttcgctgac gcgggaacca gcaatgatgc ccgcgttaaa
    16141 gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc tcaatttgcg cctgatgctg
    16201 gtttttctcc gcaagtgtct gaacctgact ggctgcccgc gccacgtcat tcaccgcttc
    16261 aaccaccgct ttgttgtagc tggcgataga caagttgctt tcggcttttg cgatatcgag
    16321 attggcgtta agacgaccac tatcgaaaat gggtagcgtc aggcctgccg taacgcccat
    16381 ttgctgcgcg gaatgacgga acagatcgct taagtgcaac gcatcctgtt gcaggaaggc
    16441 catcaggttg atgtcaggat aaaatgccgc ttttgccgca tcaatggtgc ttagcgatga
    16501 ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg gccagtaagg agtaccccag
    16561 ttcatcagga agctggcttg ccactttcgg caacgcgacc ggtttaagct tcaatgactt
    16621 tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata attttcattt tccccgcgac
    16681 atcgttgagc tgctgccggg ttttgctggc attaatatcg gtttccacac cttcaactga
    16741 agaagtaatc ccgttctgat atagctggcg atcggtcgcg ataatggtgt tctgctcttt
    16801 ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt tgccactccc agtacaggcg
    16861 ggctacgctg ccagccagca attggcgggt ttgctcgcgt tccgccgccc gtgctttaac
    16921 cgtacccagg cgggcagtaa cctccgcccg attctttccc cagatatcga gatgccagcc
    16981 cgccgttaag ccaaaagttc cgttggtgta ccacggaccg gtcgtacctg cggccggatc
    17041 gttcagagca aacggcccca ttaagccttc tgccgacatt ttttgccgct ccatatccgc
    17101 agaaaagtcg atctgcggac catcctgagt agcaattgcc ttcgcctggg cttcagctag
    17161 ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc tgtagtgcat tgttaattaa
    17221 ggaagtgagt tgattatcgt gatactccag ccaccattgg ctgtctggcc aaccattttt
    17281 cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc gtttgctggc ttaacgcctg
    17341 gcgggtttca tgcataggcg cacacccggc cagcatcagt aacagcggaa aacaggcgat
    17401 ggcgggataa aaggaatcac gattcatggg ggaataatca ggtaagaaaa ggtgcgcgga
    17461 gattaccgtg tgttgcgata tattttttag tttcgcgtgg caatacatca gtagcaataa
    17521 aacgacatat ccagaaaaat atacactaag tgaatgatat cttccgattt atcttaatcg
    17581 tttatggata acggcaaagg gcttcgtttt ttcctatact tattcagcac tcacaaataa
    17641 aggaacgcca atgaaaatta tactctgggc tgtattgatt attttcctga ttgggctact
    17701 ggtggtgact ggcgtattta agatgatatt ttaaaaatca gatatggcat ataagttgtc
    17761 aggtcaggaa ctagatattt tgtgtacttg atttgcaaac agctccggtg ttggtggcgt
    17821 caaaatcgct gaagcgttcc ccgaaggccg gggcagccca catggatgtg ggctgagggc
    17881 gcgtcttaca aggacgttac ctcgcgcccg acccggtaga cgaaggggat aagctgaagg
    17941 taccgcgcag cggcgatttt gttcgccaga gcccgggggt gcagggggag gaggcgattg
    18001 tcctccccct gcgcgctcct tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc
    18061 gcaacaagat gatgaagctg aataagcgat tcattgtctc tgataaactg cgattccact
    18121 attatcgcag cttatcgata tcaattgcct gaatagccct tgcaatatca ggggaattat
    18181 tcaacacccg aacatgctga aataattccg ttgcttcatc gtattcttta cgcaaataac
    18241 tcaaccactg tttaatccgc gcaacgtgat ataacccggt atcgccctgc ttttccagac
    18301 gggtatattt ttgcagcaaa gcaaccacct ccggccacgg cattcgcggt tcgttatatt
    18361 ttactacccg gctcaggttg ggaatattga gcgccccgcg accaatcatc actgcgtcgc
    18421 agccgctgat cgcaatgcat tgttgcgcgc tctgccagtc ccagatttcg ccgttggcaa
    18481 tcaccggaat attcagccgc tggcgaatct cgccaatcgc ctgccagtca atatgctccg
    18541 cgcggtaacc ctgctctttt gtccgtccat gcaccaccag ctccgtagca ccagcctgtt
    18601 gaaccgcatc ggcgatttca aatttcttct caccgctgtc ccagcccaga cgcactttca
    18661 cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc ttttgcaccc tggtagatga
    18721 gttcaggatc tttgagtaac gtcgccccac caccgctacc gttgaccgtt ttcgacgggc
    18781 agccgcaatt gagatccacg ccccaggaac ctaattccac cgcacgggcg gcgttctctg
    18841 ccagccattg tgggaactga cccaacaact gcacgcgcac cagcgtaccg gatggtgtcc
    18901 ggctggcgtt ttgtagctca gggcaaatgc gatgaaaaac ttttaccggc agcagttgat
    18961 ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt
    19021 cacgcaccag agagtcaagc acaccctcca tcggagccag taacacacgc atatcttcac
    19081 ccgcaaaaaa atgaggcgct atgttagcgc ctatggtcaa cggattaaag gtctgcaaag
    19141 agggattgct gcaaactgta atccctctct atgcttgatg tatgcctgag ttcgttgcgc
    19201 attgcgtaat gccggggagc gaaaaaataa gcgctgattt cttaatgtga tcggtagcac
    19261 gttttacgaa ttaattgtat gatgaatcca tctcatctgg ggtgttgatt atgagtaaga
    19321 cactgaacat tatctggcaa tatttacgcg ctttcgtcct gatttatgcc tgcctgtatg
    19381 caggcatttt cattgcttcc ctgctaccgg taaccattcc gggcagcatc atcggtatgc
    19441 tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc aaaatgggtc aatccggggt
    19501 gctacgtact gattcgctat atggcgctat tgtttgtgcc gattggcgta ggcgtcatgc
    19561 aatattttga tttgctccgc gcacagtttg gcccggtagt ggtttcctgt gcaatcagta
    19621 cgctggtggt ttttctggtg gtgagctgga gttcgcaact ggtacacggt gaacgtaaag
    19681 tcgtaggtca gaaaggatca gaagaatgat ggcaaatatc tggtggtcat taccgctgac
    19741 gttgattgtc ttttttgccg cccgcaaact ggcggcacgg tataagtttc ccctgcttaa
    19801 cccgttgctg gtagcaatgg tggtgatcat tcctttttta atgctgaccg gcatctctta
    19861 cgacagctac tttaaaggta gcgaagtgct aaacgatctg ctgcaaccgg cggtcgtcgc
    19921 gctggcctat cctttatatg agcagctaca ccagatccgc gcgcgctgga aatcgatcat
    19981 caccatctgt tttatcggca gcgtggttgc gatggtgacg gggacttccg tggcattgtt
    20041 gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg aaatcagtca ccacgcctat
    20101 tgcaatggcg gttggcggca gtattggcgg tattccggca atcagcgcag tttgcgtgat
    20161 tttcgtcggc atcctcggcg cggtatttgg ccatacattg cttaatgcga tgcgtattcg
    20221 taccaaagct gcgcgcggtc tggcaatggg gactgcctcg cacgccctcg gtacggcgcg
    20281 ctgcgccgag ctggattatc aggaaggtgc atttagttcg ctagcgctgg tgttatgcgg
    20341 gataattact tcgctgatcg caccgttcct tttcccgatt attctggcag taatgggcta
    20401 aaatttgcga tgcgtcgcgc atttttgatg taagtttcac gcgttgcata attaatgaga
    20461 ttcagatcac atataaagcc ataacgggtt cgtaaactgt tatcccatta catgattatg
    20521 aggcaacgcc atgcatccac gttttcaaac cgcttttgcc caacttgcgg ataacttgca
    20581 atctgcactg gaacctattc tggcagacaa gtacttcccc gctttgttga ccggggagca
    20641 agtctcatcg ctgaagagcg caacggggct ggacgaagac gcgctggcat tcgcactact
    20701 tccgctggcg gcggcctgtg cgcgtacgcc attgtcgaat tttaatgttg gcgcaattgc
    20761 gcgcggtgtg agcggaacct ggtatttcgg tgccaatatg gaatttattg gtgcgacaat
    20821 gcagcaaacc gttcatgccg aacaaagcgc gatcagccac gcctggttga gtggtgaaaa
    20881 agcgcttgca gccatcaccg ttaactacac gccttgtggt cactgccgtc agtttatgaa
    20941 tgaactgaac agcggtctgg atctgcgtat tcatctgccg ggccgcgagg cacacgcgct
    21001 gcgtgactat ctgccggatg cctttgggcc gaaagatctg gagattaaaa cgctgctgat
    21061 ggacgagcag gatcacggct atgcgctgac tggtgatgcg ctttctcagg cggcgattgc
    21121 agcggcaaac cgttcgcaca tgccttacag taagtcgcca agcggtgtcg cgctggaatg
    21181 taaagacggt cgtattttca gtggcagcta cgctgaaaac gccgcattca acccgactct
    21241 gccaccgttg cagggagcgt taattctgtt gaatctcaag ggttatgatt acccggatat
    21301 ccagcgcgcg gttctggcag aaaaagccga tgcgccgttg attcagtggg atgccacctc
    21361 cgcaacgctg aaagctctcg gctgtcacag tatcgaccga gtgcttctcg cttaagcctg
    21421 gtgccggatg cggcgtgaac gccttgtccg gcttgccagc cctctcctgg tgtcgaaatt
    21481 cccggcaaac agtttgccgt ttcttgcgca aaaccagcgg gtaaagtagc ctgatggaaa
    21541 ttttccttag atcgagtctc ctgcatgtta aagcgcgtgt tcctcagcct gttagtcctg
    21601 atcggcttgc tgctgttgac tgtgctcggc ctcgatcgct ggatgagctg gaaaaccgcg
    21661 ccttatatct acgacgaatt gcaggatctc ccctaccgcc aggtcggtgt ggtgctcgga
    21721 acagcaaaat attatcgtac tggcgtaatt aatcagtatt atcgctaccg cattcaagga
    21781 gcgattaatg cctataacag cggtaaggta aattatctat tactgagcgg cgataacgca
    21841 ttgcaaagtt ataatgagcc gatgaccatg cgcaaagatt taatcgctgc tggtgtcgac
    21901 ccatcagata ttgttctcga ttacgcaggc tttcgtacgc tggattccat cgtgcgtaca
    21961 cgcaaagttt tcgatactaa tgatttcatt attatcaccc aacgtttcca ctgtgagcgg
    22021 gcattattta ttgcgctgca tatggggatt caggctcagt gttatgccgt accgtcaccg
    22081 aaagatatgc tgtcagtacg tattcgcgaa tttgccgccc gtttcggtgc gctggctgac
    22141 ctttatattt ttaaacgtga accgcgtttt ttagggccgc tggtccctat tccggctatg
    22201 caccaggtgc cggaagatgc gcaggggtat cccgccgtca cacccgaaca gttacttgaa
    22261 ttacaaaaga aacaaggaaa gtagttatgg atgtacagca gttttttgtc gttgccgttt
    22321 ttttccttat tccgatattt tgtttccgcg aagcatggaa aggctggcgc gcaggcgcga
    22381 ttgataaacg ggttaaaaat gcaccggaac cggtgtatgt ctggcgagca aaaaatcccg
    22441 gactcttttt cgcttatatg gtggcatata tcggcttcgg aattttatct atcggcatga
    22501 ttgtttatct tattttctat cgttaatatt ttctcttttc tgttaatta
//
LOCUS       DAZFRJ010000061        22365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000061 DAZFRJ010000000
VERSION     DAZFRJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.061"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHKUN_002177"
     CDS             complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHKUN_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO6660428.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHKUN_002178"
     CDS             complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHKUN_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO6660429.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            810..1187
                     /gene="ybaN"
                     /locus_tag="ACHKUN_002179"
     CDS             810..1187
                     /gene="ybaN"
                     /locus_tag="ACHKUN_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO6660430.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1340..1891
                     /gene="apt"
                     /locus_tag="ACHKUN_002180"
     CDS             1340..1891
                     /gene="apt"
                     /locus_tag="ACHKUN_002180"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO6660431.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHKUN_002181"
     CDS             2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHKUN_002181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO6660432.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3284..3348
                     /gene="dnaX"
                     /locus_tag="ACHKUN_002181"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHKUN_002182"
     CDS             4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHKUN_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO6660433.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4333..4938
                     /gene="recR"
                     /locus_tag="ACHKUN_002183"
     CDS             4333..4938
                     /gene="recR"
                     /locus_tag="ACHKUN_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO6660434.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5048..6922
                     /gene="htpG"
                     /locus_tag="ACHKUN_002184"
     CDS             5048..6922
                     /gene="htpG"
                     /locus_tag="ACHKUN_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO6660435.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVELEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7103..7747
                     /gene="adk"
                     /locus_tag="ACHKUN_002185"
     CDS             7103..7747
                     /gene="adk"
                     /locus_tag="ACHKUN_002185"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO6660436.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7983..8945
                     /gene="hemH"
                     /locus_tag="ACHKUN_002186"
     CDS             7983..8945
                     /gene="hemH"
                     /locus_tag="ACHKUN_002186"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO6660437.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHKUN_002187"
     CDS             complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHKUN_002187"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO6660438.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10053..11357
                     /gene="gsk"
                     /locus_tag="ACHKUN_002188"
     CDS             10053..11357
                     /gene="gsk"
                     /locus_tag="ACHKUN_002188"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO6660439.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHKUN_002189"
     CDS             complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHKUN_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO6660440.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHKUN_002190"
     CDS             complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHKUN_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO6660441.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14842..16494
                     /gene="ushA"
                     /locus_tag="ACHKUN_002191"
     CDS             14842..16494
                     /gene="ushA"
                     /locus_tag="ACHKUN_002191"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6660442.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHKUN_002192"
     CDS             complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHKUN_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO6660443.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHKUN_002193"
     CDS             complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHKUN_002193"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO6660444.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHKUN_002194"
     CDS             18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHKUN_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6660445.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHKUN_002195"
     CDS             complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHKUN_002195"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO6660446.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21367..22299
                     /gene="glsA"
                     /locus_tag="ACHKUN_002196"
     CDS             21367..22299
                     /gene="glsA"
                     /locus_tag="ACHKUN_002196"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO6660447.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         5497 a         5976 c         5908 g         4984 t
ORIGIN      
        1 acaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgc
       61 gcgttaattt cttttcataa tcgcgtttta caatatccag cgcgcgcagc acggtgcggg
      121 ctgggatctg attttcttcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
      181 gggcattttc cagtgacata ctctctccat tgctagcggg ttaagcgcgc tagcctgttt
      241 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
      301 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
      361 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
      421 cgggcaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
      481 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
      541 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcatc gagacaagcc
      601 tgcagtgttg tcgcacgggt ttgaaaaagg tgtctgtcaa aacgggcact tagcgtggca
      661 aattgcgcta ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
      721 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
      781 agccactctg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
      841 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
      901 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
      961 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
     1021 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
     1081 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
     1141 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
     1201 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
     1261 gtcaaaggtt aaacaactgt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
     1321 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
     1381 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
     1441 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
     1501 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
     1561 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
     1621 aaccatcagt gaaacttacg acctggaata cggcaccgat cagctggaga tccacgttga
     1681 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
     1741 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
     1801 cattatcaac ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
     1861 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
     1921 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
     1981 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggtcttagcc
     2041 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
     2101 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggtacccgt
     2161 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
     2221 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
     2281 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
     2341 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
     2401 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
     2461 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
     2521 tcgcgctgtt tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcaactt
     2581 gagcacatcc tcaacgaaga acatatcgct cacgagccgc gggcgttgca attgctggcg
     2641 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
     2701 ggtgacggcc aggtttcaac ccaggcagtc agtgcgatgc tgggtacgct tgacgacgat
     2761 caggcgctgt cgctggttga agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg
     2821 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
     2881 ctgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
     2941 gccattgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
     3001 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
     3061 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cacgtatgcc tctgcctgag
     3121 ccagaagtgc cacgccagtc ctttgcgccc gtcgcgccaa cggcagtaat gacgccaacc
     3181 caggtgccgc cgcaaccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
     3241 accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa
     3301 gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
     3361 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
     3421 aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcta ccactccagt gatgcagcaa
     3481 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
     3541 gaactggcgg cgaagctggc ggcagaagcc attgagcgcg acgcgtgggc ggcacaggtg
     3601 agtcaacttt cgctaccaaa actggtcgaa caggtggcct taaatgcctg gaaagaggag
     3661 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
     3721 gcacagcaaa aactggctga agcgttgagc acgttaaaag gttcaacggt tgaactgact
     3781 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
     3841 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
     3901 cgtcggttct tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
     3961 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
     4021 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
     4081 agagatcgcg cagctggaag tcaccggcga atctggcgca ggtctggtaa aagtgaccat
     4141 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
     4201 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
     4261 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
     4321 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
     4381 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
     4441 cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
     4501 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
     4561 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
     4621 cagacggggc agttttcagg ccgttatttt gtgttgatgg ggcacctgtc accgctggac
     4681 ggcatcggtc cggatgatat cggccttgat cgtctggaac agcgtctggc agaggaaaaa
     4741 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
     4801 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
     4861 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct tgccgggcgt
     4921 cataagattc gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc
     4981 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
     5041 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
     5101 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
     5161 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
     5221 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
     5281 ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
     5341 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca
     5401 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
     5461 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
     5521 cgaaggtgaa tataccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct
     5581 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
     5641 caaatactcc gaccatatcg cgctgccggt agagctcgaa aaacgcgaag agaaagacgg
     5701 cgaaaccgtt atctcctggg agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
     5761 gtcggaaatc accgatgaag agtacaaaga gttctacaaa cacatcgccc acgactttaa
     5821 tgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
     5881 gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
     5941 actgtacgtc cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct
     6001 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
     6061 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
     6121 aatgctggaa aaactggcga aagacgacgc ggacaaatac cagaccttct ggcaacagtt
     6181 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
     6241 actgctgcgt tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga
     6301 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta
     6361 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
     6421 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
     6481 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
     6541 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct
     6601 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
     6661 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
     6721 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
     6781 acgtgcggca gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga
     6841 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
     6901 gaaccagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
     6961 cttttcatca tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
     7021 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaattcgac
     7081 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
     7141 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
     7201 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
     7261 ggatgctggc aaattggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
     7321 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
     7381 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
     7441 cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta
     7501 tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact
     7561 gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca
     7621 gatgactgca ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata
     7681 cgcgaaagtt gacggcacca agccggttgc tgaagttcgc gctgatctgg aaaaaatcct
     7741 cggctaattc gaaagcgcgc acggacagtc ccctcgcccc ctcggggaga gggttagggt
     7801 gaggggaaca ggcccgcaca agcaaactta tcagcaatct caggccggat attcattcgg
     7861 ccttttacaa aaacctcaat ctgcgctatt cctttttctg atttgacctc tcacaacaat
     7921 tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg
     7981 taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca
     8041 cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata
     8101 cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg cgctcgccgc
     8161 gtgtggcgaa gatgtacgcc tcggtctgga tggaagacgg ctcgccgctg atggtttaca
     8221 gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgcccgta gcgctgggca
     8281 tgagctacgg ctcgccatca ctggaaagcg ccgtcgatga actcctggca gagcatgtag
     8341 atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc ggtgcggtat
     8401 gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttattc
     8461 gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta cgcgcttctt
     8521 ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt ccccagcgtt
     8581 atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc gaactggctt
     8641 ccgcactggg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg
     8701 aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag
     8761 gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga
     8821 ttgccgagca aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat gaatatattc
     8881 cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt gccgcgtatc
     8941 gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca
     9001 tcattcgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg aactcacagg
     9061 gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc agcggatcga
     9121 actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacagac
     9181 agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca
     9241 aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc acggaatccc
     9301 gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa tcgatctgtt
     9361 tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa
     9421 agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacaag
     9481 cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg gggtaatcaa
     9541 taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat
     9601 cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg
     9661 gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc atgtaagctc
     9721 tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg taatactgtc
     9781 gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga agagtattca
     9841 caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt tccggcttca
     9901 tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc
     9961 cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt
    10021 cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca
    10081 aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg
    10141 aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt gaagcgaaag
    10201 tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg
    10261 atgacgtagc cgaagcgctt tatcaggagc taaaacagaa aaacctgatt acccatcagt
    10321 ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg gacgaccgtt
    10381 cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc tatcgttacc
    10441 tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat ggcccgattg
    10501 gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agtccaggcc
    10561 acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga gcctcggcac
    10621 tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca
    10681 tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca
    10741 agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtttcta
    10801 tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg
    10861 catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct
    10921 tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc
    10981 cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc cacaaggatt
    11041 gccagaatcc gctgcgtgta tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa
    11101 tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat gacattaccg
    11161 ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc acctggttaa
    11221 cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga
    11281 accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt
    11341 cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat
    11401 ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg cctacatatt
    11461 tttcatattt tacatccggc aaccaccgtt taccccgtca ccacctcacc cgccggtggc
    11521 gtttccagca gttccagcat ggtacgggcg atttcacgct cgcccatcac tacctgattc
    11581 gcaccacgtt cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca
    11641 atatccggat ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata accgttggga
    11701 atcgtcagga tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc
    11761 gccgcattgc ccaatactgc gcggaccccg cgctctcgca gctcatcaac acgggttcgt
    11821 gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg
    11881 cctacacgac cgtaacccac cagtagcgca tggttgcaaa tatccactgg gatctgcttc
    11941 tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat
    12001 ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc ccctgccagt
    12061 actaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata
    12121 aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt acgttgggag
    12181 tgaccaaaca gtcgcaccag gaaaaatgcg gctaacgact taccaaacag aataatcgcc
    12241 agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg
    12301 acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca
    12361 cggtgactca gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag
    12421 gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac
    12481 agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca cggcaccaga
    12541 cggcgaccta ccagcatcat aatggcgata aatgcgatca ctttgccgat ggtgatcccc
    12601 atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgccactgcg
    12661 ggcagcaaca ccagcgtcag aaccattacc aggtcttcca caatcaacca accgatggcg
    12721 atttgcccac gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg
    12781 gtactggcgg tggaaagaca taaaccgaac acgataccgg tcattaacga ccagcccagc
    12841 acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcgccggga
    12901 atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac
    12961 atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag
    13021 cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata
    13081 cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg
    13141 gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt
    13201 ttggccggga aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttca
    13261 tgaataatta ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggatcgga
    13321 aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa
    13381 gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc
    13441 aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat actggtgtga
    13501 tcggcgataa gccccagaac tgccgctccc agacctccca taccaaaagc aaaaccgaaa
    13561 aagagtccag aaaccatacc gatacgtccc ggaagcagct cctgagcgta aaccagaatg
    13621 gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc
    13681 aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc
    13741 acatatttcc gcccaatttt atcccctaca ggcccgccga tcaccgtacc tgccgcaacg
    13801 gcaaacagga aggcaaacag atgaagctga gcattctgga tagataatcc gaatttttgc
    13861 atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg
    13921 aggattaaca gaatgctgac cgccagtacc actttgttgc gcggcagtgg attgataatc
    13981 gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg gctgatttgc
    14041 gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca
    14101 taaggcgcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa gttgccgccg
    14161 acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg ggctacgcga
    14221 gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgccgc cagcagaact
    14281 gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa gcacatgcca
    14341 attggcaacg accacggcat cggatattta tcggtccagt agccgaccac tggttgcagt
    14401 agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa
    14461 aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc
    14521 agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac
    14581 gcagccgcgc ccgccacagg ctggggttgt tcactcattg ccataggaaa gtcacttttt
    14641 cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta
    14701 tgcgtcgcct gaaggaagtc tcaacgccga atacagaatt tctaatctgg atgcagattt
    14761 atcttcaccg gacgcagact tgtctatgat gtcgcgtcat actatttttc aacacgttga
    14821 aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta
    14881 accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa aacctacaaa
    14941 attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa
    15001 tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa
    15061 ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtctgac
    15121 ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg
    15181 atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga aaagtgggcc
    15241 aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg cctgtttaaa
    15301 ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg tcattgggct gacaaccgat
    15361 gacacagcaa aaattggtaa cccggaatac ttcactgatg tcgaatttcg taagcccgcc
    15421 gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc
    15481 gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc accgggcgat
    15541 gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg
    15601 caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg
    15661 ccatgcaagc cagatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa
    15721 tacgtgggac gggctgattt tgagtttcgt aatggcaaaa tgaaaatggt taactaccag
    15781 ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg
    15841 ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag
    15901 aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc
    15961 gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct ggcagcccaa
    16021 atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc
    16081 gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg caatgtggtg
    16141 gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt cgcgcagatg
    16201 aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggc
    16261 agactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg
    16321 gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga taacaaaccg
    16381 ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc
    16441 tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa
    16501 agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt
    16561 ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc
    16621 gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg
    16681 ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac gctgcgcgac
    16741 catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc
    16801 cagttgaccg gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc
    16861 caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc
    16921 gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt
    16981 tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg
    17041 aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg
    17101 caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata
    17161 ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc
    17221 aacatttgcg gtaaattccc ttctccatac aggtgtagtg caccgaccgc gaccacatat
    17281 cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg atgcatcagc
    17341 acatcgtaca gcgactgact gaacgtattg ggcagcgtta tttcattatt ttgcggcggt
    17401 gcattcagcc accagctcat catttgttgc agcaaccgtg cattggtgtg ccagtgggtc
    17461 agtgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat ggcaatctgg
    17521 ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc ctgcaatagc
    17581 tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga
    17641 accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat gcccatctcc
    17701 tggctaatgt gctgcagatt ttggagttgc tcctcgctaa tgcgctcttc cagcgcctcg
    17761 caggcaggca aattggcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc
    17821 gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta
    17881 cccatatgaa tactgccaat cagatgaaaa tggcgattgc cgggaagggt gatatcgatg
    17941 gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac
    18001 agatccatac gacctccctt tgtgaaatat catgctagcg cgcggggagg gatggtgcaa
    18061 atggagttgg ggacttgttt aatgtgtttg tatgattcag tatattcttg tatcgctatt
    18121 cacagggaag caacagttaa aaactatgaa acaggcaaca agaaaaccga cgacacccgg
    18181 agatattctt ctctatgaat atctggtacc gctcgatttg aaaatcaatg agttagcaga
    18241 gttgctgcat gttcatcgta atagcgtcag tgcactgatc aataacaatc gtaaactcac
    18301 cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt tttggctaaa
    18361 cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt
    18421 gggacggatt gaaacagtgg ctgaatactt ggcacgccgt gaagagcgtg caaaaaaggt
    18481 cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttta cgccgcatcc
    18541 ggcagtcatg cgtcgatgcc aaatgtgcca ccctaaagca gcgcatccgc aatgacatac
    18601 ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg taatcgacga
    18661 gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtaa acggccacaa
    18721 aataccggcg gcgaccggaa taccgatact gttgtagata aaggcaccga gcaggttctg
    18781 cttcatgttg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacgc ccatcaggct
    18841 atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cccccatcgc
    18901 aataccgaca tccgcctggg ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc
    18961 cacctgacgt ccttcacttt gcaggcgttt aatcgcttcg gctttaccgt ccggcagcac
    19021 cccggcgatc acttcatcaa tcccggcttc tttggcgatc gcattggcgg tggttgggtt
    19081 atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca gcgccgccac
    19141 gctatcacta cgcaatggat cgcgtactgc cagcagggct accgctttcc cgtcaaccgc
    19201 cagcagcaca ggcgttgccc cttgcgatgc ctgagcagta atctccgctt cgatagcttt
    19261 ggtaccaacc tgttgctcat ttaacagcgc ctgattgccc agcaataacg catgaccttc
    19321 agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg
    19381 catatcacct gctttatcga ggatcgcccg tgccagcgga tggctggaac cttgctccag
    19441 tgccgccgcc agacgcaatg cctgcgcttc atcaacatca gcaaacgttt tcactgcgac
    19501 aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg tatcgagtgt
    19561 actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc
    19621 gacgccggaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac aggcgataat
    19681 cagtaccgtg gtggcaatca ccagggtata aacaatttgc ggtgccggac caaagaagta
    19741 ccagattgcc gcactgacca gcgcaatcac caccaccaca ggtacaaata cggctgagat
    19801 tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca ccatgcgaat
    19861 gatccgtgac agcgtggtat ggctgccaac cgcactggca cgaaacagca cgctaccgtc
    19921 ctgtaccact gtcccggcat ggacgctttc gccttcgcct ttttgctgcg ggattggttc
    19981 gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc catctaccgg
    20041 cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttccg ccagaggcac
    20101 gcttttttca ccttcgtcag taaccaggcg cgccgtcggt ggggttaaat cgagtaattt
    20161 ctccagcgcc ttagaagaac gctgacgtgc gcgtgcttcc agcatatggc cgagattgat
    20221 cagaccgata atcatcgcgc tggcttcgta ataaagatgt cgcgcttcca tcgggaacca
    20281 ctgcggccac aggttgacgc tcatcgaata gagccacgcc acgccagtac ccagcgccac
    20341 cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc ggtaaaaatg
    20401 gccgccggcg aaaaccatca ccgccagggt tatcaggccg ataaccaacc acaggctgcg
    20461 gttgtcagcg gtgaccatca tgttatcgcc aatcatcccc cagaccatca ccgggatacc
    20521 caccgccagt gcgacaattg cctgccagcg gaagcgcttc atcgtagcaa cggcggtttc
    20581 ctgctggcgc tcacggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt
    20641 ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc
    20701 cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg
    20761 ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc
    20821 ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga
    20881 ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat
    20941 ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag tgatagacac
    21001 atccgcttgt tccacatccg ggcgctgttc gagactttct ttcacgcgtt taacgcagtg
    21061 accgcaggac aggccgtcca gggtcaggtc gataatttgt gacataaaac actcctttaa
    21121 gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag gtcaagaaat
    21181 taataaacca ggcgagtaaa agtccgtaaa gattaaaaaa ttggctcaat ttgcatcagg
    21241 attagacatt tatctctttg ttttcctgta gttaagttgc gggtgctaag ttaaatcaag
    21301 atgcctgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa aaggagttaa
    21361 caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta cacccaattt
    21421 cactcactta acggcggaca aaatgccgac tacattccct ttctggcgaa tgtacctagt
    21481 caactggcag cagtggctat tgtgacctgc gatggcaaca tctatcgcgc gggtgatagt
    21541 gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct tgcgttagaa
    21601 gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg attgcccttt
    21661 aactcagtta tcgccttaga gttgcatggc ggcaaaccac tgtcgccact ggtaaatgct
    21721 ggcgctattg ccaccaccag cctgattaac gctgaaaata ttgaacaacg ctggcagcga
    21781 attttacata tccaacagca actggctggt gagcaggtag cgctctctga tgaagtcaac
    21841 cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct gtactccgcc
    21901 ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca gtgctccacg
    21961 ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat
    22021 ccgctgacgc ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg
    22081 atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg tttaccgggc
    22141 aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg aattgccgcg
    22201 ttctcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg
    22261 gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac acggaaggta
    22321 ataacggtaa caaacctctc ggtctatgga acgtcgtttc catcg
//
LOCUS       DAZFRJ010000062        22142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000062 DAZFRJ010000000
VERSION     DAZFRJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.062"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..944
                     /gene="ygcG"
                     /locus_tag="ACHKUN_002197"
     CDS             72..944
                     /gene="ygcG"
                     /locus_tag="ACHKUN_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6660448.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1004..2302)
                     /gene="eno"
                     /locus_tag="ACHKUN_002198"
     CDS             complement(1004..2302)
                     /gene="eno"
                     /locus_tag="ACHKUN_002198"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO6660449.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2390..4027)
                     /gene="pyrG"
                     /locus_tag="ACHKUN_002199"
     CDS             complement(2390..4027)
                     /gene="pyrG"
                     /locus_tag="ACHKUN_002199"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO6660450.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4255..5046)
                     /gene="mazG"
                     /locus_tag="ACHKUN_002200"
     CDS             complement(4255..5046)
                     /gene="mazG"
                     /locus_tag="ACHKUN_002200"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO6660451.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5117..5452)
                     /gene="mazF"
                     /locus_tag="ACHKUN_002201"
     CDS             complement(5117..5452)
                     /gene="mazF"
                     /locus_tag="ACHKUN_002201"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO6660452.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5452..5700)
                     /gene="mazE"
                     /locus_tag="ACHKUN_002202"
     CDS             complement(5452..5700)
                     /gene="mazE"
                     /locus_tag="ACHKUN_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO6660453.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5778..8012)
                     /gene="relA"
                     /locus_tag="ACHKUN_002203"
     CDS             complement(5778..8012)
                     /gene="relA"
                     /locus_tag="ACHKUN_002203"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO6660454.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8060..9361)
                     /gene="rlmD"
                     /locus_tag="ACHKUN_002204"
     CDS             complement(8060..9361)
                     /gene="rlmD"
                     /locus_tag="ACHKUN_002204"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO6660455.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9418..12174
                     /gene="barA"
                     /locus_tag="ACHKUN_002205"
     CDS             9418..12174
                     /gene="barA"
                     /locus_tag="ACHKUN_002205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO6660456.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12406..13746)
                     /gene="gudD"
                     /locus_tag="ACHKUN_002206"
     CDS             complement(12406..13746)
                     /gene="gudD"
                     /locus_tag="ACHKUN_002206"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO6660457.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13767..15107)
                     /gene="gudX"
                     /locus_tag="ACHKUN_002207"
     CDS             complement(13767..15107)
                     /gene="gudX"
                     /locus_tag="ACHKUN_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO6660458.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15109..16461)
                     /gene="gudP"
                     /locus_tag="ACHKUN_002208"
     CDS             complement(15109..16461)
                     /gene="gudP"
                     /locus_tag="ACHKUN_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO6660459.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16896..17345)
                     /gene="yqcA"
                     /locus_tag="ACHKUN_002209"
     CDS             complement(16896..17345)
                     /gene="yqcA"
                     /locus_tag="ACHKUN_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO6660460.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17363..18145)
                     /gene="truC"
                     /locus_tag="ACHKUN_002210"
     CDS             complement(17363..18145)
                     /gene="truC"
                     /locus_tag="ACHKUN_002210"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO6660461.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18145..18474)
                     /gene="yqcC"
                     /locus_tag="ACHKUN_002211"
     CDS             complement(18145..18474)
                     /gene="yqcC"
                     /locus_tag="ACHKUN_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO6660462.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19096..19641)
                     /gene="syd"
                     /locus_tag="ACHKUN_002212"
     CDS             complement(19096..19641)
                     /gene="syd"
                     /locus_tag="ACHKUN_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO6660463.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19709..20557
                     /gene="queF"
                     /locus_tag="ACHKUN_002213"
     CDS             19709..20557
                     /gene="queF"
                     /locus_tag="ACHKUN_002213"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO6660464.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20669..22033
                     /gene="ppnN"
                     /locus_tag="ACHKUN_002214"
     CDS             20669..22033
                     /gene="ppnN"
                     /locus_tag="ACHKUN_002214"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO6660465.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5434 a         5906 c         5251 g         5551 t
ORIGIN      
        1 taatgataac gaatctaaaa ttaccgatgc cgccgtcaat ttatttatcc agatttaatg
       61 agaaatttat aatgcgctat ttcatactaa tgttcacttt cgtgtgttcc tttgttgcag
      121 cccaaccaac tattgtccca caattacagc aacaagttac ggatcttacg agtagcttaa
      181 actcacaaga aaagaaagaa ctgacgcaca agttagaatc tattttcaat aatacccaag
      241 tgcaaattgc tgtattaatt gttcccacaa ccaaagacga gacaattgaa caatatgcta
      301 caagagtttt tgacaattgg cgtttaggag atgccaaacg taatgatggg atactgatca
      361 ttgttgcctg gtcagatcgt actgtccgca tccaggtagg ttatgggctg gaagaaaagg
      421 taaccgatgc tctggctgga gatatcatcc gtagcaacat gatacccgca tttaaacaac
      481 aaaaatttgc tcagggatta gagctagcta taaacgcttt gaataaccaa ctcacttcac
      541 aacaccaata tccgactaat ccttcagaaa gtgaatcagc gtcttccagt gatcattatt
      601 actttgctat tttttgggta tttgcagtga tgttcttccc tttctggttt tttcatcaag
      661 gcagtaattt ttgtcgcgca tgtaaaagtg gagtctatat ttcagcaatc tatcttttag
      721 atttattcct gttctccgat aaaatttttt ccattgctgt attttccttc ttttttactt
      781 tcaccatatt tatggtcttt acctgtttat gtgtacttca gaaaagagca tcaggtaaaa
      841 gttatcattc agacaacagc ggttccggtg gaggctcaag tggcggcgga tttagcggcg
      901 gtggcggttc ttctggaggt ggcggcgcat ccggccgctg gtaactcaac cataaaaaat
      961 gccagcccgg aggctggcat ttttaaatca gataaagtca gtcttatgcc tggcctttga
     1021 tctctttacg accgttgtac ggtgcttttt cgcccagagc ttcttcgata cgaatcagct
     1081 ggttgtattt agcaacacgg tcagaacggc tcatagaacc agttttgatc tggcctgcag
     1141 cagtaccaac agccaggtca gcgatggtag cgtcttcagt ttcgccagaa cggtgagaga
     1201 taactgcagt gtagccagca tctttcgcca tcttgattgc agccagagtt tcggtcagag
     1261 aaccgatctg gttgaatttg atcaggatgg agttagcgat acctttttcg ataccttctt
     1321 tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac cagctggatt ttgtcgccca
     1381 gaactttggt ctggtatgcg aaaccgtccc agtcagattc gtccagaccg tcttcgatag
     1441 aaacgatcgg gtactgtttg gtcagttctt ccaggaagtg agtgaattct tcagaggtga
     1501 acgctttgtt gccttcgcca gccagaacgt atttaccgtc tttgtagaat tcagaagctg
     1561 cgcagtccat cgccagagtg atgtctttgc ccagttcata accagcagct ttaacagctt
     1621 cagcgataac agccagagct tcagcgttgg aacccaggtt cggcgcatag ccaccttcgt
     1681 caccaactgc agtgttcatg cctttcgctt tcagaacttt tgccaggtga tggaaaactt
     1741 cagaacccat gcggatggct tctttcacag ttttcgcgcc aaccggctga atcatgaatt
     1801 cctggatatc aacgttgttg tcagcgtgct caccaccgtt gatgatgttc atcatcggaa
     1861 ccggcataga gtatttgccc ggagtaccgt tcagttcagc gatgtgctcg tacagcggca
     1921 tacctttagc agctgcagca gctttggcgt tagccagaga tacagccagg attgcgttcg
     1981 cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat catgatcttg tcaatgccag
     2041 cctgatcttt agcatctttg ccaatcagcg cctgagcgat cgggccgttt accgcagcaa
     2101 cagctttggt tacgccttta cccaggaaac gggatttgtc gccatcgcgc agttccagag
     2161 cttcacggga accagtagaa gcacctgacg gagcagctgc cataccgacg aaaccaccct
     2221 ccagatgtac ttcggcttca acagtcgggt taccacggga gtcgatgatt tcacgaccga
     2281 tgatttttac gattttggac attaggtttt cctcaagtca ctagttaaac tgaaactcca
     2341 gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa cttttttact tacttcgcct
     2401 gacgtttctg gaactcgctg gcggctttca caaagcctgc aaacagcggg tgaccatcac
     2461 gtggagtaga agtaaactcc ggatggaact ggcaagccac gaaccacggg tgattcggaa
     2521 cttcgatgat ctcgaccaac tgatcatccc cggaacggcc cgcaacgcgc agacctgcat
     2581 cttcaatctg tttcaacagc atgttgttga cttcgtaacg gtgacgatga cgctcaacaa
     2641 ttgtcggcgc attgtacagc tggcgaacca ggctatcgtc aaccaactgg cactgctgtg
     2701 cgccgagacg catggtaccg ccgagatcgc tcttctcgct acgaacttca acgttgccgt
     2761 tttcatcgcg ccactcggta atcagcgcca caaccgggta cttacagtct ggcacaaatt
     2821 ccgtagagtt ggcgttctcc atgttggcaa catggcgagc gtaatcaatt aacgccacct
     2881 gcatacccag gcaaatgccc agataaggaa tattgttctc acgcgcaaaa cgcgcggtcg
     2941 taatcatgcc ttctacgcca cgatagccga aaccgccagg tacgaggatt gcgtccagac
     3001 ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga atcgatcagt ttgatgttga
     3061 cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc gatcactgat ttataagcat
     3121 ccggcagttc aatgtacttg ccgaccatac cgatggtgac ttcacttacc gggttcgctt
     3181 cttcgaagat aacctgttcc cattcggaca gattcgcttc cgggcagttt aagctgaatc
     3241 gtttacaaat ataatcgtcc agcccctgag atttcaacag gcccggaatt ttatagatgg
     3301 aatcgacgtc tttcagagaa ataaccgctt tttccggaac attacagaac aatgcaatct
     3361 tcgcacgttc gttcgccgga acagcgcgat ctgaacgaca aatcaggatg tcaggctgga
     3421 taccgatgga gagcagctct tttacagagt gctgagtcgg tttggttttg acttcaccag
     3481 acgctgccat gtacggcacc agcgtcaggt gcataaacag agtgtgctca cggccaattt
     3541 caacagccat ctggcgaatc gcttcgagga acggcaagga ttcgatatca cctactgtac
     3601 cgccgatttc taccagtact acgtcatgac cttcgccacc ttccagcacg cgctctttga
     3661 ttgcgttagt gatgtgcgga ataacctgca cggttgcgcc gaggtagtca ccgcggcgtt
     3721 ctttacgcag aacgtcagag tagatacgac ccgtggtgaa gttgttgcgg cggctcattt
     3781 tggtacgaat gaaacgctcg tagtgcccca ggtccaggtc ggtttcagcg ccgtcttcag
     3841 taacgaacac ttccccgtgt tggattgggc tcatagtacc tggatcgacg ttgatgtacg
     3901 gatccagttt catgatggtc acattgaggc cacgggcttc aagaatggct gcgagggagg
     3961 ctgcggcaat gcctttaccc agagaggata cgaccccgcc ggtcacaaaa atatagttcg
     4021 ttgtcatgct gaacctgaga agttaggttg aaaagacgat ggaataacca ggacgggaaa
     4081 gcagtatacc cgaacatgac ctgtgccaca aactttcatt atccctcctc ttcgccagcg
     4141 cactattgaa atcaggagtg agaaaatagc ccctttgggg taaatgtttt tgacgcaaat
     4201 caagcgcttg tcatttaaaa aatgacacaa atggcgcttg accgcgtaat tcccttagag
     4261 atcaatttcc tgccgtttta cctgttgcca gacttcttcc attgtttcga ggtcaacacc
     4321 tgtcatttcc agtccacgcg cggcaacaat acgctccact tcgcgaaaac gacgctcaaa
     4381 tttttcgttc gctttttgca atgcgatttc cgcttttgtc cctaaatgac gagccagatt
     4441 gaccgtggca aacagcaggt cccccatttc ctcctccagt ttagcctggt cgacaacagc
     4501 ctgccgcgct tcgtacatca cctcgtcgat ctcttcgtag actttatcga ctaccggacc
     4561 aagcgtcgtc caatcgaagc caacgttggc gcaacgtttc tggatttttt gcgcacgcat
     4621 taaagccggt aaactacgag gaatatcgtc cagcgccgaa tgctgcgctt tctgcgcgcg
     4681 ctcttcggtt ttgatttgct cccaacgggc aagcacttca ctactgtttt cggcagaact
     4741 atcagcaaaa acatgcggat ggcgacgctc taatttatcg ctaatagcag cgcaaatatc
     4801 attaaagtca aagcgccctt cttcctgagc catttgcgcg taaaacacca cctggaatag
     4861 cagatcgccc agttcaccgc gaagatcgtc aaaatcttca cgggcgatgg cgtccagcac
     4921 ttcgtaggtt tcttcaaggg tgtaaggcgc aatggtggca aatgtctgct ctttatccca
     4981 cgggcagccg ttttccggat cgcgcaggcg ctgcataata gtgagcaaac ggtcgatttg
     5041 attcattgaa ttgtcctgaa aattgcgggt ctgtcaggtg gaaacctgtg accagaatag
     5101 aagtgagtta gtaacactac ccaatcagta cgttaatttt ggctttaatg agttgtaatt
     5161 cctctggggc aactgttcct ttcttcgttg ctcctcttgc ccgccaggcg atacttttta
     5221 cctgatcagc taacgctacg ccatcacgtt cctgaccgga taaaacaact tcgaacggat
     5281 atccttttga ttgcgttgta taaggaacac acagacacat acctgttttg ttgttgtaca
     5341 tgaaaggact caggacaaca gctggacgat gtccagcttg ctcgctacct tttgtcgggt
     5401 caaaatcaac ccaaatcaga tcgcccatat cgggtacgta tcggcttacc attaccagac
     5461 ttccttatct ttcggctctc cccagtcgat attctcgtgg aggttttccg gcgtgatgtc
     5521 gttgaccagt tcagcaagcg taaatacggg ctctttacgc actggctcaa taattaattt
     5581 gccatccacc aggtcaatct tcacttcatc atcaatattc agattgagcg cctgcattaa
     5641 cgtagccggg atccgcaccg ccggtgaatt tccccaacgc tttacgctac tgtggatcat
     5701 aaccctttcc tcaaaccgct atcatatgta gatacagtat atatcaatct acattgtaga
     5761 tacgagcaaa tttcggccta actcccgtgc aaccgacgcg cgtcgataac atccggcacc
     5821 tggttgagtt tacccagcac gcgccccagc acttgcaggt tgtaaatctc aatggtcatg
     5881 tcgatggtcg ccagttgctg tttggtgtcg ctacggctgg caacgccaag cacgttcacc
     5941 ttctcgttgg cgagaatggt cgtgatatca cgtaacaacc cactacgatc attagccacc
     6001 acgcggacca ccagcgaata tccggcggag tagctctcac cccataccgc gtcaacaatg
     6061 cgttctggcg catgggagcg cagttccgcc agttgttcgc aatcggcgcg gtgtactgaa
     6121 ataccgcgcc cctgggtaat gaagccgaca atctcatctc caggaatcgg ctggcagcag
     6181 cgcgcgatgt ggtgcatcag gttgccaaca ccttcgacca ccacgcgacc gttatcttta
     6241 ctgcggtttt gcggcgtgta gcttttttgc tgaagttgct tcagcgcggc ggcgtcctgc
     6301 tcttcggcac tcggcttatt aaattgcgat tgcaggaagt tcaccatctg attgagacgg
     6361 atatccccgc caccaatcgc cgccagcaac tcgtcgacat cattaaagtt gtaacgcggc
     6421 agcagatgtt tttctgcttc tttcaggctg atccccagat gttccagctc gtcgtcaagg
     6481 atttgccgcc cagccagaat gtttttgtca cggtcctgtt tacggaacca ggcgtgaatt
     6541 ttcgaacgcc cacggctggt tgtgacgtaa ccgaggtttg ggtttaacca gtcacggctg
     6601 gggttcggct gtttctgggt gataatttca atctggtcgc ccatctgcag ctggtaggtg
     6661 aacggcacaa tgcgcccgcc aatttttgcc ccgatgcagc ggtgtccgac atcactgtgg
     6721 atgtggtaag cgaagtccag cggcgttgat cccgcaggca aatcaacgac atcacctttc
     6781 ggcgtaaaga catacacccg gtcgtcaaag acctggctgc gtacttcgtc gagcatttcg
     6841 ccggaatcag ccatctcttc ctgccacgca atcagtttac gcagccaggc aatccggtct
     6901 tcatgtcccg aacgtgcgcc gccagctgcc gcgccctctt tatatttcca gtgcgcagca
     6961 acacccaact ctgcatcttc atgcatctgt ttggtgcgga tttggatctc aacggttttg
     7021 ccacctggcc ccagaaccac ggtatgaata gactgataac cgtttggttt cgggttagcg
     7081 acataatcgt caaactcatc cggcaggtgg cgatagtgag tgtgcactat ccccagtgcg
     7141 gcataacaat cctgtaaacg ctcggcgaca atacgtaccg cacgcacatc aaacagctca
     7201 tcgaaggcga ggttcttttt ctgcattttg cgccagatac tgtagatgtg tttcggacga
     7261 ccatacactt cagctttaac gccttcagct ttcatctcag cgcgcagatg gccgacaaac
     7321 tcttcgatat agtgttcgcg gtcgagacgg cgttcatgca gcagttttgc gatgcgtttg
     7381 tattcggtcg ggtggagata acggaagcag taatcttcca gctcccattt cagttgccca
     7441 atcccaagac ggttcgccaa cggcgcgtag atattggtgc actcttttgc cgccagtacg
     7501 cgttcatctt ccggcgcatc ttttacttcg cgcagatgag caatacgctc cgccagtttg
     7561 atgaccacgc agcgaaaatc atcgaccatc gccaataaca tccggcgaac gttatcgacc
     7621 tgttcggagg aaacagaatc agtgtgcgtc gctttcagct ggcggatcgc cgccatatca
     7681 cgcacgccgt gaataaggtt aacgaccgac ttaccgacgc tctcacgcag cacatcttcg
     7741 ctgactacgt tggcatcagc cagagggaac agcagcgccg cccgcagcgt gtcaatgtcc
     7801 atacttaatg tcgagaggat ctccaccatc tcaacaccac gccacaataa cagactggca
     7861 tccggatgcc cctgcgtctg ttgcagacaa tacgcccagg tttcggctaa gcactcacac
     7921 gacttctggc tggtaatacc cagacttgcg atccattttt ccggatcaaa ttcaccagcc
     7981 ttattgatat gtgcacttct taccgcaacc attgtcctct cctttaggga ccagacctgc
     8041 cgaaatcggc aaatcgtaac tatttaacgc gcgagaaaag taccatcgat tccagatgtc
     8101 ccgtgtgtgg gaacatatcc agcatcgcca gtcgcgcaat ggtatatcct gcttttaata
     8161 acgcttcgct atcccgagcc agcgttgcag ggttacagga tacataaact atacgaatag
     8221 gttccagttt tataatttgc tgcataacac ctgcggcacc tgctcgcgcc gggtccagca
     8281 acactttatc gaagccgttt ttcgcccacg gctgctttgt gacatcttct tcaagatttt
     8341 cgtgataaaa cgtcacattc tgtaaaccat taagacgcgc attctgctgg cctttttcca
     8401 ccagcgccgg aacaccttct acaccgacca cactggctgc ttgtttcgcc aatggcagtg
     8461 taaagttgcc cataccgcag aacagatcca gtacgcgatc ttcaggttgc acatccagcc
     8521 attccaacgc acgcgctacc attttttggt tcacacccgc attgacctga ataaaatcgc
     8581 gcgggctaaa agttaagcgc aacccgtttg agtcatacca gggcatctca ccagagacgg
     8641 tttcgagtat ctcactatcg ggggcgagat acagatccag gccttcagaa tgcgaaaagc
     8701 gttccagttt ttcgcgatct gccgaactta gcggtgcggt atggcgcaaa atcatcagcg
     8761 tgccgctggt tgcctgtacc agttcaacat gaccaagatg gcgcataact tgtaagctgc
     8821 ccaggcatgc cctgactttg ggcagcaatg cttcaagttg gggcactaaa atggggcatt
     8881 gtttaacgtc gacaatgtca ctggagcccg ctttgcgaaa ccccatctga agttgctgtg
     8941 ttttcggtaa gtagttcaga cttaaacgcg cgcggcggcg atagccccag ggaacatcgg
     9001 cgatcacttc agagacatcg tgtttcatta atcgggcgag tgccgcactt ttgcttcgct
     9061 gctgtaaatc cacgctggcg tgttgttgct gacagccgcc gcatacgcca aaatgaggac
     9121 agcgtggtgt ttcgcgttcc gggctatcgc ttaaccggcg tacgacttta gcgcgggcat
     9181 actgtttttt atcttcagta acagcaactt ccgcgttttc ctgcggcaat aatccgggga
     9241 taaataacgt tttgccgtta tgtcgcgcca cgccctgacc aaaagagtcg aggtcgttga
     9301 ctgaaacggt tatgatctga cgcgtcgtcg tgcgtcgttt tgcagagtag aattgcgcca
     9361 ttggcgagac tttctcaatt taacagtgtg accttaattg tcccataacg gaactccatg
     9421 accaactaca gcctgcgcgc acgcatgatg attctgatcc tggcaccgac cgtccttatt
     9481 ggtttattgc tgagtatctt tttcgtcgtg catcgctata acgacttgca gcgtcaactg
     9541 gaagatgccg gtgccagcat tattgagccg cttgcagttt ctactgaata tggcatgagc
     9601 ctgcaaaatc gcgaatctat cggtcagtta ataagcgtac tgcatcgtcg ccattccgat
     9661 attgttcgcg cgatttcggt ttatgatgaa aataaccgac tctttgtcac ctccaatttt
     9721 catcttgatc cctcatcaat gcagctcggc agcaacgtgc cgtttcctcg ccagctcact
     9781 gtcactcgtg acggcgatat tatgatcctc cgcacgccga ttatttctga gagttactcc
     9841 cccgacgaat cgcccagtag cgatgccaaa aatagtcaaa atatgttggg atatattgcg
     9901 ctggagctgg atcttaaatc ggttcgcttg cagcaatata aagagatctt tatttccagc
     9961 gtgatgatgc tgttttgtat cggtattgcg cttatttttg gctggcgctt aatgcgcgat
    10021 gtaaccggtc cgattcgcaa catggtgaat accgtcgacc gcatccgtcg cgggcaactc
    10081 gacagccggg tggaaggatt tatgctcggc gagctggata tgctgaaaaa cggtatcaac
    10141 tcgatggcaa tgtcgctggc tgcttatcac gaagagatgc agcacaatat cgaccaggcg
    10201 acgtccgatc tgcgtgaaac gctggagcag atggaaattc agaacgttga gttagatctg
    10261 gcgaaaaagc gcgcccagga agcggcgcgt attaaatccg agtttctggc aaatatgtca
    10321 cacgagctgc gtacaccact gaatggtgtt attggcttta cccgcctgac gctgaaaaca
    10381 gaattaacac caacgcagcg cgatcacctg aatacgattg aacgttcggc aaataatttg
    10441 ctggcaatta ttaatgatgt tctcgacttc tcgaaactgg aagcaggtaa gctgattctg
    10501 gaaagtattc cattcccact acgcagcacg ctggatgaag tcgttactct gctggcacat
    10561 tcttctcacg ataaagggtt ggaactgacg ctcaatatta aaagcgacgt gcctgataac
    10621 gtgatcggcg acccactgcg attacagcaa atcatcacta acctggtggg gaatgcaatt
    10681 aaattcaccg agaatggcaa cattgatatt ctggtagaaa aacgtgcgct gagtaatacc
    10741 aaagtgcaga ttgaagtgca gattcgggat accggcattg gtattcctga acgcgatcaa
    10801 tcgcgcttat tccaggcctt ccgacaggct gatgccagta tttcccgccg tcatggtggc
    10861 accggtctgg ggctggtgat tacacaaaaa ctggttaatg aaatgggcgg cgatatttcg
    10921 ttccatagcc agccgaatcg cggttcaact ttctggttcc acattaatct cgatctgaac
    10981 ccgaacatta ttatcgaagg gccatccacc cagtgcctcg ccggtaagcg cctggcctat
    11041 gtcgaaccaa actccgcagc agcgcaatgc acgctggata ttttaagtga aacgccgctg
    11101 gaagtggttt atagcccaac gttctccgcg ctgcctcccg cgcattacga catgatgttg
    11161 ttaggcatcg cggtgacctt ccgcgagccg ctaacaatgc aacatgagcg attagcgaaa
    11221 gcggtatcga tgaccgattt cctgatgctg gcacttcctt gccatgcaca agtcaatgca
    11281 gaaaaactca agcaagatgg tatcggcgcg tgtctgctga aaccattaac acctacgcgc
    11341 ctgttgcctg ccctgacgga attttgtcat cacaaacaaa acacgctttt gcctgtaacc
    11401 gatgaaagca agctggcaat gacagtcatg gcagttgatg acaaccccgc taacctgaaa
    11461 cttatcggcg cattgctgga agatatggtg caacatgtgg aactttgcga tagcgggcat
    11521 caggcggttg aacgggcgaa acagatgccg ttcgatttga tcttaatgga tattcaaatg
    11581 cctgacatgg atggcattcg ggcctgcgag ctcatccacc agctccccca tcagcaacaa
    11641 acgccggtta tcgcggtaac ggcgcatgca atggccgggc aaaaagagaa gctgcttggc
    11701 gcagggatga gcgattatct ggcgaaaccg attgaagaag agcgattgca taatttgttg
    11761 ttgcgctaca agcctggcag cggtatttcc tctcgcgtcg tgacgcccga agtcaacgaa
    11821 attgtggtga acccgaatgc gaccctcgac tggcaactgg cactacgcca ggcagcagga
    11881 aaaaccgatt tagcgcgcga tatgctgcaa atgttactcg atttcctgcc tgaagttcgc
    11941 aacaaagttg aggaacagct ggttggagaa aacccggaag gcctggttga tttgattcat
    12001 aaactgcatg gcagttgcgg ctatagcggt gtgccgcgta tgaagaatct ctgccaactg
    12061 atcgaacaac agctacgtag cggtactaaa gaagaagatt tggagccgga gctgctggaa
    12121 ctgttggacg agatggataa tgtagcgcgc gaagccagca aaattctcgg gtaacggacg
    12181 tcttgtcgag tagcaatcgg ctttttatga cgctggtttc agattggtga caaagtgcct
    12241 gtgtttatgc cggatgcggc gttaacgcct tatccggcct acaaaaccaa ttaaattcaa
    12301 tgaattaaaa aatgatgtag acctgataca tagcacaaca ggctattttg cgtttagcat
    12361 cagtctcaag ccggctccag atagagccgg ttttggtttt ctgtcttaac gcaccatgca
    12421 cgggcgcttg ttatcgaacg tccagccagg aatcagatac tgcattccca tcgcatcgtc
    12481 acgcgcgcca agcccgtgtt tctgatacag ctcatgggct ttcatcactt gatccatatc
    12541 gatttctaca cccagccccg gtttttccgg cacctgaacc agaccgcctt tgatctcaaa
    12601 cggttctttg gtcaggcgct gattaccttc ctgccagatc cagtgcgtat caatagcagt
    12661 aattttaccc ggtgcagcgg cggcaacatg ggtaaacatc gccagggaaa tatcgaagtg
    12721 gttgtttgag tgtgaacccc aggtcaggcc aaattcatgg cacatttgcg ccacacgtac
    12781 cgaaccttgc atcgtccaga aatgcggatc cgccagcggg atatcaacgg attgcaggga
    12841 gagcgtatgg cccatctgcc gccagtcggt ggcgatcata ttggttgcgg tcggcaggcc
    12901 agttgcgcga cggaactcag ccatcacttc acgcccggag aaaccttgct ccgcaccaca
    12961 cggatcttct gcataagcca gcgacccttt caggtattta ccaattttaa tcgcttcgtt
    13021 cagcgaccag gcaccgttag gatcgagcgt aatacgcgcc tgcgggaagc gttgcgccag
    13081 tgccacaata gactcggcct cttcttcccc ggccagtaca ccgcctttca gtttgaaatc
    13141 gttgaagcca tatttttcat acgccgcttc cgccaggcgc accaccgcat ccggcgtcat
    13201 cgcttcttca tgacgcaggc gataccagtc gcatgagtca tccggctggc tttgatacgg
    13261 cagcggtgtg gctttgcgat caccgacgaa gaacagataa ccgagcattt cgacttcgct
    13321 acgctgttga ccatcgccca gcagcgatgc cacgtttacc cccagatgct gccccagcag
    13381 atccagcatt gccgcttcta tcccggtaac tacatgaata gtggtacgca aatcaaatgt
    13441 ctgcaaaccg cgcccaccag catcacgatc ggcaaaagta ttacgcacca gcgtcagaac
    13501 gtttttgtat ttacccagcg ttttacctac caccagcgga atcgcatctt ccagcgtttt
    13561 acggattttc tcgccgccgg gaatttcccc tacgccagtg tgaccagaat tatctttgat
    13621 aatcacaata ttacgcgtaa agaacggtgc gtgtgcacca ctcagattca tcagcatact
    13681 gtcatgaccc gccaccggga tgacctgcat ttcagtaaca acaggcgtcg taaattgaga
    13741 actcatattt atgtcctttt tcagaatcaa tgacggccga aaacgggacg tttacggtca
    13801 aaggtccagc cggggatcag gtactgcatc ggacctgcgt cgttacgcgc accgccaggc
    13861 agacgtttat aggcctcatg tgccttttgt acctgttccc agtccagttc cacgcccaga
    13921 ccgggcgcat caggaacggc aatttttccg tttttaatct ccagcggatt tttggtcagg
    13981 cgacaatcgc cctcctgcca aatccagtgg gtatcgatag cggtaggatt acccggtgcc
    14041 gccgcgccca catgggtaaa catcgccaga gagatatcga aatggttatt agaatggcag
    14101 ccccaggtca gcccccagtc gtcgcaaagc tgcgccacac ggactgcacc ggaaagcgtc
    14161 cagaagtgcg gatcggcaag tggaatatct accgcattga gcatcaccgc atgacccatt
    14221 tcgcgccagt tggtggcgat catgttagtc gcgacgggca agccggtcgc ccgccgaaac
    14281 tccgccatca cttcacgacc ggagaaaccc tgttctgcgc cgcatggatc ttcggcatag
    14341 gtaagaacat cattcagccc tttgcataga gaaatggctt catcaagtag ccaggcaccg
    14401 ttgggatcaa cggtaatccg cgcatcaggg aagcgtttct tcaatgcccg aacagtgtcg
    14461 atttcttgct cgccaggtaa caccccgccc ttaagtttga aatctttaaa gccatagcga
    14521 tcctgtgagg cttccgccag acgcacaacg gcttcgctgt tcatcgcttt ctgatggcgc
    14581 aactgatacc actcatggtt gcccggcgta ttttccagat aaggaagatc ggttttggtc
    14641 cgatcaccga tataaaacag ataaccgagg acggtaatag tctcgcgttg cttgcctggc
    14701 cccaacagtt cgcagaccgg aacattcagc gccttaccta gcaggtcaag caaggcggct
    14761 tccagcgccg ccacggcatt aacgcgcaat tcaaaagtcc aggcaccttt gccgaaggta
    14821 tcaaaatcgg ctgcctgatt acctttatgc acctgctgaa ccactttatt caggcgcgca
    14881 acttcctggc ccagaaccat cggaatagca tcgaccagtg tttgataaat cacctctccg
    14941 cccggcgctt caccaatgcc ggtatgcccg gcgttatcgg tgagtaccac aatattgcgg
    15001 gtgaaatatg cgttatgtgc cccaccaata ttaagcaaca tgctatcgtg cccggcgacc
    15061 ggaatgactt tcatatcagt aataacagga ctggattgtg tcgtcatctt attgccccgc
    15121 gacaggtttc agctcgatac gcttgatatc gcccaccagc accaggtagc tcagtaccgc
    15181 gattaaggca tgaacaccaa cataaatcag cgccccatta aaggagccag tcgtgccaac
    15241 gatataacca attgcgattg gcgtgacgat gccagaaatg ttaccgaaca tgttgaacag
    15301 gccaccggaa agaccgctga tctcttttgg cgcggtatct gccattactg cccaacccag
    15361 cgccccgatg cccttaccga agaaggccag cgccataaag ccgatgatca tccactcaac
    15421 gttgacgtag ttgcagaaca ccatcaccat cgacagcaac atgcccatta cgatcggtgt
    15481 tttacgcgca atgttcagcg atcccgtgcg gcgcatcagc cagtcggaaa taatcccacc
    15541 cagcacaccg ccgataaaac cgcaaaccgc cggaacggaa gccacaaagc ccgctttcag
    15601 aatcgacatc ccacgcgcct gcaccagata aaccggaaac caggtaatga aaaagtaagt
    15661 cagggcgttg atacagtact gaccgatata aacgccgatc atcatccgtg acccgagcag
    15721 ttgtttgatc tgcccccact tcacgctgaa cggcacttta actttggtgt tttgctgatc
    15781 catattgatc agcgcgccac ccgcggcgat gtactccagc tctttctgat ttacccccgg
    15841 atgttgattt ggctcgtgga tgactttcaa ccagataaag ctgatgacaa tccccagacc
    15901 gcccataaag aagaagacgt gtgaccagcc cacttcatgc gtcagccagc ccataatcgg
    15961 cgcgaagatc accgttgcga agtattgagc ggagttaaaa atcgacaccg ccgttcccct
    16021 ttcctgcgcc ggaaaccagg ccgcaacaat gcgactgttg ccggggaaag atggcgcttc
    16081 agcaagcccg accaggaagc gcagcgtaaa cagggcaacg ataatgccga atccactaaa
    16141 gatatcgacg aagccttgca gcaaggtaaa catcgaccag ataaagatcg accagaagta
    16201 gacgcgtttt gaaccaaaac gatccagcaa ccaaccacca gggatctgcc cgataacata
    16261 agcccatgag aaggcagaga acacatagcc cattcccacg ggatcaaggc cgatatcttt
    16321 ggccatttcc gaaccagcaa tagaaagcgt agcgcggtcg ccgtagttga aggatgtgac
    16381 gataaacaac atcaccacta tccagtaacg agcattcgtg cgtttttcca cactgctcgc
    16441 agcctgactt aaagaactca ttgttgcact cctgaaaatt cgcgttagcc acgctcactc
    16501 tggactgcga catcgccagg aaatcagagg tgacgtaggg tgttttttgc cgtttttata
    16561 ggtcgttcgc cgaatacggc gcgtgtttat atctggcaat agcagtataa aaagcgcgcc
    16621 atagcggctc accgtgcaac aacacaacat taatgcgttc aatgaggccc gattttggca
    16681 ttagccctgg acggtggaat ccacttcacg gaaatgaaaa caagaacaag aaaggaaggg
    16741 taaaaacgaa gaaataaaga agagtatgaa atggatcgct tgactccagg caaacgccag
    16801 taaaaatccg cgctatgaag cagtttttac tggcatttgc ctgaaaagat tcgattcagc
    16861 accgctaaaa cgacatttac cgctcgctga acatatcagg acaacagcgt gccccactgt
    16921 tcgacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagt
    16981 atttcgccaa cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca
    17041 ttgcagaaat ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc
    17101 tggaagccca gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg
    17161 ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg
    17221 cttaattcag gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct
    17281 gcttcttccg ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc
    17341 gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac
    17401 tgaactcaac cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct
    17461 gcatccaggt gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg
    17521 tcaatgacaa ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc
    17581 tgcgattctg gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg
    17641 caagatgtcg gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt
    17701 agcgcgtggt cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat
    17761 aatgcgtcac tgctggctgc gagcctttat cttcgcgggc aaatttatca gcgattttgt
    17821 ccagttcttc caccagtgga taatccagaa ctgcttcttc catcaaccag ccgcgcacaa
    17881 tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg
    17941 cctcgctgga taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcgg
    18001 taaaaacgtg ctggccaatc tgatcacgca cggtttgcat gaccactact ttctcgtcgc
    18061 gatccagcca gctgcggtga accagccagc cggagggttt atttaccgca accagccatt
    18121 catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt
    18181 ttccagctca gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata
    18241 atagggtgca acggcaaaag cgcccggtaa cggctgtttg ttatctaaca aatcgtgcat
    18301 tcgcgggatc agcacccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg
    18361 ttgggtacta ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg
    18421 cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca
    18481 acctcaataa gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt
    18541 cacagcgctc ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc
    18601 ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct
    18661 cacaccaccc cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa
    18721 tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat
    18781 cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag
    18841 tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc
    18901 tttttcgcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa
    18961 agttaagaat taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt
    19021 aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca
    19081 gatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga
    19141 ggctaaatgc gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt
    19201 acacaccgag atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag
    19261 ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc
    19321 gtcttcactc caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc
    19381 cccggcaaac tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc
    19441 gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc
    19501 atcttcggta gttgaaataa tgcacggtga aggaacaccg tacagttcct cacttaacgg
    19561 ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc
    19621 ttgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt
    19681 tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc
    19741 gggcctgact cttggaaaat caaccgatta ccgggatacc tatgacgcca acctactgca
    19801 aggcgttcca cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt
    19861 tcacggtacg gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc
    19921 gcaggttgcc gtcggtcacg ttgaacttga ttacaccagc gcaaatctga ttgagtcgaa
    19981 gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt
    20041 gcgccagacg ctggagcgcg acttaagcac ttgcgcccag ggtaaggtta gcgtggcgtt
    20101 atatcgtctt gatgaactgg aaggccagcc gataggtcat ttcaatggca cctgcattga
    20161 tgaccaggat atcactatcg ataactatga attcactact gactatctgg agaatgccac
    20221 cagcggtgaa aaagtagtgg aagagacgct tgtcagccac ctgctgaaat caaactgcct
    20281 gatcacccat caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga
    20341 cagagaaaaa ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca
    20401 gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga aattaagcgt
    20461 ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga
    20521 ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct
    20581 ggattcacgc agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg
    20641 acgatattcg tcccataagg agtttttctt gattacacat attagcccgc ttggctccat
    20701 ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta
    20761 tcaactgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag
    20821 caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg
    20881 cgtaaagctg gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg
    20941 cgctttgcag gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat
    21001 ccataatacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct
    21061 ggtcttttcc atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt
    21121 ggtctgctgg ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg
    21181 aaaccagctg ggcctgcgtg agctgaatat ctgcaccggc tgtggtccgg gagcgatgga
    21241 agcgccgatg aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt
    21301 tattggtatg accgagccgt cgattatcgc cgctgaaccg cctaacccgc tggtcaacga
    21361 attgatcatc atgccagata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg
    21421 tatcattatc ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat
    21481 tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga
    21541 gagcgccgac tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc
    21601 gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa
    21661 atcgatgccg ctggtgaaag aaaatcgccg cgacactggt gacgcttaca gctttaactg
    21721 gtcaatgcgc attgcaccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc
    21781 taatctgaag ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc
    21841 gttctccggt attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt
    21901 tggtccttac aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca
    21961 gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga
    22021 aatctgcacg taatctcccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg
    22081 tcacttactc atcaactcat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga
    22141 ca
//
LOCUS       DAZFRJ010000063        21913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000063 DAZFRJ010000000
VERSION     DAZFRJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.063"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..670)
                     /locus_tag="ACHKUN_002215"
     CDS             complement(<1..670)
                     /locus_tag="ACHKUN_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO6660466.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHKUN_002216"
     CDS             complement(873..1274)
                     /gene="nikR"
                     /locus_tag="ACHKUN_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO6660467.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHKUN_002217"
     CDS             complement(1280..2086)
                     /gene="nikE"
                     /locus_tag="ACHKUN_002217"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO6660468.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHKUN_002218"
     CDS             complement(2083..2847)
                     /gene="nikD"
                     /locus_tag="ACHKUN_002218"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO6660469.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHKUN_002219"
     CDS             complement(2847..3680)
                     /gene="nikC"
                     /locus_tag="ACHKUN_002219"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO6660470.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHKUN_002220"
     CDS             complement(3677..4621)
                     /gene="nikB"
                     /locus_tag="ACHKUN_002220"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO6660471.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHKUN_002221"
     CDS             complement(4621..6195)
                     /gene="nikA"
                     /locus_tag="ACHKUN_002221"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6660472.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHKUN_002222"
     CDS             complement(6306..6893)
                     /gene="acpT"
                     /locus_tag="ACHKUN_002222"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO6660473.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6948..7997)
                     /gene="yhhT"
                     /locus_tag="ACHKUN_002223"
     CDS             complement(6948..7997)
                     /gene="yhhT"
                     /locus_tag="ACHKUN_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6660474.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8129..9346
                     /gene="yhhS"
                     /locus_tag="ACHKUN_002224"
     CDS             8129..9346
                     /gene="yhhS"
                     /locus_tag="ACHKUN_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660475.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHKUN_002225"
     CDS             complement(9350..9907)
                     /gene="dcrB"
                     /locus_tag="ACHKUN_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO6660476.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9980..10645)
                     /gene="yhhQ"
                     /locus_tag="ACHKUN_002226"
     CDS             complement(9980..10645)
                     /gene="yhhQ"
                     /locus_tag="ACHKUN_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO6660477.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10866..11111
                     /gene="tusA"
                     /locus_tag="ACHKUN_002227"
     CDS             10866..11111
                     /gene="tusA"
                     /locus_tag="ACHKUN_002227"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO6660478.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHKUN_002228"
     CDS             complement(11213..13411)
                     /gene="zntA"
                     /locus_tag="ACHKUN_002228"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO6660479.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13485..14111)
                     /gene="yhhN"
                     /locus_tag="ACHKUN_002229"
     CDS             complement(13485..14111)
                     /gene="yhhN"
                     /locus_tag="ACHKUN_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO6660480.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14252..14611
                     /gene="yhhM"
                     /locus_tag="ACHKUN_002230"
     CDS             14252..14611
                     /gene="yhhM"
                     /locus_tag="ACHKUN_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO6660481.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14614..14883)
                     /gene="yhhL"
                     /locus_tag="ACHKUN_002231"
     CDS             complement(14614..14883)
                     /gene="yhhL"
                     /locus_tag="ACHKUN_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO6660482.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHKUN_002232"
     CDS             complement(14873..15469)
                     /gene="rsmD"
                     /locus_tag="ACHKUN_002232"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO6660483.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHKUN_002233"
     CDS             15619..17115
                     /gene="ftsY"
                     /locus_tag="ACHKUN_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO6660484.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHKUN_002234"
     CDS             17118..17786
                     /gene="ftsE"
                     /locus_tag="ACHKUN_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO6660485.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHKUN_002235"
     CDS             17779..18837
                     /gene="ftsX"
                     /locus_tag="ACHKUN_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO6660486.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHKUN_002236"
     CDS             19082..19936
                     /gene="rpoH"
                     /locus_tag="ACHKUN_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO6660487.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20207..21310
                     /gene="livJ"
                     /locus_tag="ACHKUN_002237"
     CDS             20207..21310
                     /gene="livJ"
                     /locus_tag="ACHKUN_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO6660488.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHKUN_002238"
     CDS             complement(21509..21892)
                     /gene="panM"
                     /locus_tag="ACHKUN_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO6660489.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5180 a         5822 c         6082 g         4829 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagtaccc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggactcagg cgaagctctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacaccgc
      241 cgcgcaccag ccagagtgat tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga gaatgaacgg cagcgggccg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac ccagcagcgg attgaccgga tggccggacg
      541 tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaata cgtaccccgg
      601 ctgaaccctg aacaatgcta ccgccatact gcgtcatgtc accctgacgc gccgccggtt
      661 ttccgctcat cgtaaaatcc ttttctatta ttttaatgac agtatcttca tacgaaaaag
      721 cgaagaaaaa cttgtggtca caatgaattg aagattttta ggaatatatt tagaacgtta
      781 caaaagagaa ggttcggata gggtatttag agaggaatca ggtgtgctag tggccctttg
      841 ttgatcgttc accaaaggac ccaataatag actcaatctt ccttcggcaa gcactgcaaa
      901 tgcccgtgcc gcacgccgcg ctgggcgata acgtcatcgg caaaatgctg cacgtcgccc
      961 atatcacctt tcagcacggc gatttccaga cagtcgtcgt gattgatatg cacatgcagc
     1021 gtggcgacgg agagatcgtg gtgatggtgc tgggtggaga caatgcggct ggctaagtcg
     1081 cgtttttcgt gttcatacac ataggagagc accgcgaaac cttgcgtgcc gtgctgctga
     1141 gtggcctcct gggccagagc gctacgcaga atatcgcgga tagcttcgga acgattgtta
     1201 taaccacgac gctggctcag gctgtccagc gtctccagta aatcgtcatc aagcgtgatg
     1261 gtgactcgtt gcatttgcgt taaacctttt ctgtggtgcg acggcgcacg gggaatgcgg
     1321 gtaataccgc gttttgtagc acacgtccgg cgtcagagga aaaggttaat ttatctccca
     1381 ccacctgggt ttcgacgatt tgtccgttgt ccatcaccat cacccgctga cagaagcgtt
     1441 ccaccaggcg taagtcgtgg gtgatgaaca ggcaggcggt gccaaactgt tgttgtagct
     1501 ttttcagcag gcgaatgacg cccgcctgta acacgagatc aaggttagaa acggcttcat
     1561 ccagaatcag tagtttcggt tcgaccgcca gcgcgcgagc caggcagacg cgctggagct
     1621 ggccgccgct taactgcggt gggcgtttgt cgagaacgct gtcatcgaga tcgaccgcct
     1681 tcagcatttc gctggcgcgc gccagttgtt cggatttttt cagtgatagc aggtggcgca
     1741 tcggttcacg caggatctcg cgcacggttt tgcgcggatt cacggcgctg atggagtcct
     1801 gaaataccat ctgaatatcg cggcggaacg ctttacgctg ggcgcgattg agtttcgcca
     1861 gcggttcgcc acgccagcta atattcccct gcgcgggcga ttctaaaccc accagcaacc
     1921 gcgcgagggt acttttcccg cagccgctgc gccccagcag ggcgacggtt tcgccgcttt
     1981 tcagggtcag ggaaacgtta ttcagcaccg cctgatgttg atgttttccg ttaaatccac
     2041 cgtgcgcata gtgatgggaa aggccggaga tgttaagtaa agtcatgatg ccagctccat
     2101 accgtagagg gcgagatgag cggaaaccag gctgcgcgtc actgtatgtt tgggggcgtt
     2161 aaacagcgtt tctacatcgc cctgttcgac aatcttaccg tcagacatca ccgccacgtc
     2221 atccgccaga cgcgccacaa cgcccatatc atgggtcacc agcagcattc ccggcgcttg
     2281 tttttgcata atgctttcca gcagatcgag gatgcgcgcc tgtgctacca cgtcgaggtc
     2341 ggtggtcggt tcatcggcga tgataaacgg tgattcacac agcaccgcca tcgcaatcat
     2401 catgcgctgc aacatgccgc cgctcatctc gaacgggtac agcttcagca cgcgcgcggc
     2461 gttttccagc cccaccgctt ctatggcagc ggtaagcgta gcgtcatcgg cgggtttccc
     2521 taacgccagg caggtttccc gcgcgtgggt gtgcatggtg tgcagtggat taaaggcgct
     2581 gcgcgggttc tgcatgatgg tggcaatttt gatgccgcgc agggcgcaag gcgaaaccgg
     2641 tttgccatcg gctaaaattt cccccgccgt ctggcgaacg ccagcgggca aaatgcccag
     2701 cgtcgcggcg caggttaatg atttcccgct gccgctaccg ccgactaacg ccagcacgcg
     2761 cccgcgttgc agggttaacg atacaccgtg taccagcggc tgcgcggcct gtagcgcgat
     2821 attacgtagt tcaatctgtt gcggcattag tgtgcgtgct ccgtcaccag atgagggtcc
     2881 agatgatcgc gcagtgcgtc acccaccagg ttaaaggcca tcacgctgat aaacagcgcc
     2941 agccccggcc agaacatttg cagcggctgg gtccagatat actggcgcgc gtcgttaatc
     3001 atcacgcccc attcggcggt cggcgcggtc acaccgaggc caaggaaaga catccccgcg
     3061 acgtgcagca tcatatggcc gatatccagc gttgccagca ccagcagcga agggatcacc
     3121 gcgcctgcca gatgatcgac aaacacccgc acatggcccg caccggaaag ccgtgacgcc
     3181 agcacaaact cgcgttggcg tagtgaaatc accaggctgc gcaccatgcg tgcataccac
     3241 gcccagtgcg acagggcgat ggcgataatt acgttggtca gcccggtgcc gagcacgcca
     3301 accataaaga acgacagaat cgaggtcggg aaggtcataa acatatcggc gacgcgcatg
     3361 gtggcctgat caacgcgccc gccaatcaac ccggcgctgc cgccaataac cagccctaat
     3421 gtcagcacca gcagcaggca ggccattacc gaaccgagcg acacgcgggt cgctgtcatc
     3481 agccgcgaga aaatatcgcg acctaagtga tcggtgccca gccagtgctg cgcatccggc
     3541 gaaagcaggc gcgacggcaa atcaatcgcc tgggggtcat acggcagcca ccactggctg
     3601 gtgagcgcaa tcagcgccag cagggcgata atgatcagcg ccaggcgtac cgaccagcgg
     3661 gaagagagga aaaagttcac gcgtgcgctc cttcatgacg acgaatgcgc gggtccagcg
     3721 cggcgttgag caaatcgaca atcaaattac agaccacaaa aaccaccacc atcatcagcg
     3781 taaagcactg gatcaccgga tagtcacggt taaaaatcgc cgacaccgca tagcgcccga
     3841 cgcccggcca ggcaaagatg ttttcgataa tcatcgtccc gccaatcagt tcgccgatat
     3901 gcatccccac ggcggtgatc atcggcagcg aggcattgcg caggatgtgg cgacgttcgg
     3961 tctgtttgtc gttcaggccg cgcagacgcg cccaggtgac gtgacgctga ccggcgacgt
     4021 ccagcatact ggcgcgcagt aaacgcgcgt taatcgccag cgacataaag gcaatggaaa
     4081 ccgcaggcaa aatgatgtgc tgccagccgc cgtaacccat cgcgggtagc cattgcagat
     4141 acaccgaaaa cgccatcacc agtaaaaacg ccagccagaa gttaggcatc gacacgccaa
     4201 gaaacgcgat gaaacgtacg gcgaaatccg gcagacggtc gcgatggcgc gccgcccaga
     4261 taccgagcgg tacggaagtg agcagaatta ataccagcgc cgcacctgca agttccagcg
     4321 tggcgggcag gaagttcagc atatcgtcca gtaccgggcg ttggctggcg aatgagatac
     4381 caaagtcaag atgcagcgcc ttccacaacc aggtaccgta ctggacgtac agcggctgat
     4441 ccagcccgag catggtgcgg gtagaggcca gcatctccgg cgtcggcggc aggttagata
     4501 aacgcagata atcgagcgcc gggtcgccgg taccgaggcg cagcatcaga aaaatgatca
     4561 ccgaggcggc gagcaccatc gggatcagca gcagaaagcg gcgtaatacg taacgcaaca
     4621 ttaaggtttc accggtttaa tctgttcgaa cggaatttcg gtggcgatcg gcgcgtaggg
     4681 gatgttaccc agctccggtt ttgataccac catcattgag atgtaactga taggcagata
     4741 aaccgcctcg tcatgcagac gggtcagaat gtcgcgatac agcgcctgac gttgcgtttc
     4801 gtcatgggtc gccagcactt cgccgatctc tttatcaatc agcggtttgt cggctaatcc
     4861 ttgctgtgcc tggaagtcag cgtgtgacgg tacgcgcatt gaactgagga aggcgtgtgg
     4921 atcatatggc gcgccccagg tgcggtggaa aatcatgcca aaacgaccgt cgcgctgacg
     4981 agcatagata ctgctctctt cttcgccaat cagcgagaca tctgcgccaa tctggcgcat
     5041 atcagcctga atgatttccg ccatcgattt gcttaacgca tcggtgccga tgaacgacag
     5101 ttcaatgcgc agcggctgac catttttctc gcggatgtct ttgcccgcag gcagcgtcca
     5161 accggctttt tccagcaacg ctttcgcttt ctgcggatcg tactggcgcg gtttcaggcc
     5221 gaggttggcg tagggcacag aaggggcaaa cagggtgtcg gcgacctgct gggtgccata
     5281 caacgcgtta tcaatcagcg attttttgtt taccgcgtaa ttaagagctt cacgtactgc
     5341 cagctcgttg gtgggggctt tggcggtatt gagcgccagc atcacggttt cgatcggctg
     5401 tgacagttgg gtgtggtaag ccggattctg gctaaagcgg gcgaaggtat cgagcggtaa
     5461 taacccttcg tttccgtaca gcaggtcgat atcgccagtt tcaaacgcca ccgcgcgggt
     5521 agtcgggtcc gggatgacgt taaaggtgat ctttttaatc gctggctttt cgccccagta
     5581 gttttcgtta cggacgaaga catcgtactg attcagtttc gattcctgca aaatccacgg
     5641 tccggtgcca atcggcgctt taattccgtt catggtttca tggtttttaa actgcgaggg
     5701 agcgataaaa cggaaaggac ggggcagggc cagttcttgc aggaaaggat agtaggcgct
     5761 tttcagggta atttgcagct ctgttttact gagtgcttta acatcaacaa tctggtttgc
     5821 cagctccagc caggcgtgac gttgacggtt atcgagcact gcgcggaagt tttctgccgc
     5881 cgcctcggca tcgaacggtt caccgttgga gaatttcacg tcatcacgca gggtgaaggt
     5941 ccaggtttta ccatcttctg aatgagtcca gctttttgcc agccacggga tcaccgaacc
     6001 gtctgcctga tatttcacca atggttcata aaccatgctc tgggcgaaca tctggttagg
     6061 cgtgtaaagg tgcgggttta gtggcccgac attcaccggc caggcggtgg tgatttcatc
     6121 tggtgcagcg gcatggacga taaaagacgc acaagccagc agcgcaaata gagtgcggcg
     6181 gagtgtggag agcatggtaa ccccaatgga ttaaaataga tggcggaaat aagtatgacg
     6241 attttaagta ttcgtcatac tgattacctg ttctggatca atagatgggc agtcgggtgg
     6301 gcggatcagt taactgaatc gatccattgc acactgtcgg cggtgagcgt aaagggagta
     6361 ggggtgcaga tcgccaggct taaattttca agctgacaat ggctgactga cagcgaggag
     6421 tgataggtgc tgtctacgct gacgatttgc caggcgctgc cgccacgctg tttaacgatg
     6481 gcttctttgc gcgtccagat gcgccagaac atttccagtt gctgatcagg atgcacggcg
     6541 tccatctcag cgtgttcccc gaggctgaat acggcgttcg ccagccagtg ccagttggcg
     6601 cgcgggcgaa tcacttcgat atcgcagccg acttcgcctt catcactcaa cagcagggcg
     6661 atatcgtcac cgctatggct taagttgaac catagcggca tttccggcgc aaatgcaggt
     6721 ttgccttgtt cgccatagat gatctccggt agcggggaaa gcgtgtgcga aagcaatgca
     6781 cgccccgcca gccagcgttc gcgtcgtgga ccttgcggtg cttgctcgcg taaacccggt
     6841 ggcagtggag ctgcgcttaa ggtcgaaact ttccccagaa ctatccgata catatcaggg
     6901 ccaacgttta atggaaaatg aaagtgcgta tcgtatcact tgtcgcctca tcccggtaac
     6961 cgacttttcg gtctgcctgg ccccagtaaa atcgccagtt tgctaccgcc tttggtggtt
     7021 tccatccaga ttttacacac actggttaat ggcaccgaaa gtagcatccc taccgggccg
     7081 agcagccatc cccaaattaa caatgaaaga aataccacca tggtggacat ccccaggcga
     7141 tggcccatca tccgtggttc taaaatattg ccgatgacca tatggaccac taaaaacaat
     7201 gcgccgacca gaatacattc gtaaacacca ttaaacagca gcacctgaat cattggcggt
     7261 acggcggaaa ttaccgcgcc gatattgggc acgtagttga gcaaaaacgc cagtactgcc
     7321 cacatcagcg caaactgcac ccccatcagc tccagcccca gccagacgat gacacctgtc
     7381 cataaactga gtagcgtctt caatgcaaga tagtgcgaaa cgcctttaag tgcgcggtgt
     7441 aatcctgcga tgtgaatctg tgggttattc agcgcaaaac gcattttgta agggacgtgg
     7501 cgcacttcaa acagcataaa aactacggtc atcaccagca aaagcacgct cgccattgcc
     7561 ccggaaagcc cggtcattag cgctgtggtg aaggtaacca ctttttccga gtccatccgc
     7621 tgcagcattc gctccggcga catatgcaaa ttaagaaaag gcaacatctc ctgcaattta
     7681 aaaagtttgc gcgtcaactc cttattaaac ttcggcagca tagagataaa ttcgttaaac
     7741 gatgccgcca gtacgccgac cagcgcagtt agtgcgatca gcatcaccac caccacaatc
     7801 gtaatggcaa tggggcgttg tactccccga cgaataaacc aggtgacgag cgggttgagg
     7861 acgatggcaa aaaacagcgc cagtaacagc tgcacaatga tatctgccgc tgcgtgaatg
     7921 cccgcgagga tcactaccag cgaggccagc ttgagcagaa tgtgcatgcc cgttttatcg
     7981 ggttgagggg tttccattgg ggcttccttg tgactttttg tattaagtgt agcgggagtc
     8041 acgccagcaa tattctgatt cttactgctg attttcacgt cagcacgtgg taaaaatgaa
     8101 acactgttgt aaaaatgtgg tgatcctcat gcccgaaccc gtagccgaac ccgcgctaaa
     8161 cggattgcgc ctgaatttgc gcattgtctc cattgtcatg tttaacttcg ccagctacct
     8221 caccatcggg ttgccgctcg ctgtattacc gggctatgtc catgatgtga tgggctttag
     8281 cgctttctgg gcaggattgg ttatcagcct gcaatatttc gccaccttgc tgagccgtcc
     8341 tcatgccgga cgttacgccg atttgctggg ccccaaaaag attgtcgtct tcggtttatg
     8401 cggctgcttt ttgagcggtc tgggatatct gacggcagga ttaaccgcca gtctgcccgt
     8461 catcagcctg ttattacttt gcctgggacg cgtgatcctt gggattgggc aaagttttgc
     8521 cggaacggga tcgaccctgt ggggcgttgg cgtggttggc tcgctgcata tcgggcgagt
     8581 gatttcgtgg aacggcattg tcacttacgg ggcgatggcg atgggtgcgc cgttaggcgt
     8641 cgtgttttat cactggggcg gcttgcaggc gttagcgtta atcattatgg gcgtggcgct
     8701 ggtggccatt ttgttggcga tcccgcgtcc gacggtaaaa gccagtaaag gcaaaccgct
     8761 gccgtttcgc gcggtgcttg ggcgcgtctg gctgtacggt atggcgctgg cactggcttc
     8821 cgccggattt ggcgtcatcg ccacctttat cacgctgttt tatgacgcta aaggttggga
     8881 cggtgcggct ttcgcgctga cgctgtttag ctgtgcgttt gtcggtacgc gtttgttatt
     8941 ccctaacggc attaaccgta tcggtggctt aaacgtagcg atgatttgct ttagcgttga
     9001 gataatcggc ctgctactgg ttggcgtggc gactatgccg tggatggcga aaatcggcgt
     9061 cttactggcg ggggccgggt tttcgctggt gttcccggca ttgggtgtag tggcggtaaa
     9121 agcggttccg cagcaaaatc agggggcggc gctggcaact tacaccgtat ttatggattt
     9181 atcgcttggc gtgactggac cactggctgg gctggtgatg agctgggcgg gcgtaccggt
     9241 gatttatctg gcggcggcgg gactggtcac aatcgcgtta ttactgacgt ggcgattaaa
     9301 aaaacggcct ccggaacacg tccctgaggc cgcctcatca tcttaaaact tactgaataa
     9361 ccagcgtatt aatgatgttt tctgcggtgg tctgcgcttt ttgctgatcg tcagctggca
     9421 gagtaatttg catggtcagc agttgattac ccacgttacc cagaataacg gaagagtacg
     9481 ccgtctggcc tttcgcggag ataatactgt ctaactgctg cattttgtga cctttcagct
     9541 caatggcttt attggttacc acttgcagct gcggatcgcg gctacgttgc tgatcttcca
     9601 gacgcttcgc cagcaccgcc agatcttctt ttggatcatc gcccatgatg acgatgactg
     9661 ctttctgccc ggtggcgtcg gaccagacat gcatgttatt ggcctgcgtt cccagcttac
     9721 cgctctggtc ggtcatatcc gctggcagcg agaaacttaa cttgccatca agcaggttga
     9781 cgggattccc ggtagcgtta ctttccgcga ccgaaccctg cgccgtagcg ttagtgtctt
     9841 tatcatcaca ggccgcaagc cccataacca gcaggccaat tccgacatat ttaaccagat
     9901 tgcgcattga cttcttcctt tcgataaacg gccataacgg ctcattcatc catcttatca
     9961 caactctgat aacgaacctt taactcgcct gcaaagcgtt gatttcggat ttatctgcca
    10021 gtcttttcaa cagcatattg agtaatacgc catacattgg caggaagaaa acgatactga
    10081 ttaacacttt gaaacagtaa tcgaccagcg cgatttccat ccagtgttca gccataaagg
    10141 catccgggct acgccagaag gcaatgaaga aaaaggccag cgtgtcgctg acgttaccga
    10201 acagtgtgga cgctgtcggt gccagccacc agcggcgact ttgacgcagg cggttaaaaa
    10261 cgtgcacgtc gaggatttgc cccagcgcgt aggccatgaa actggcggtg gcgatacggg
    10321 cgacaaacag gttgaagtgg gcgagtgcgc cgaatccctg ccatgaaccc atatagaaca
    10381 gcgacgagat gacataggag attaataacg cagggatcat taccgcgaag ataatgcgtc
    10441 gggccagcgg tgcgccaaaa atacgcacgg tcaggtcggt agcaagaaaa ataaacggaa
    10501 agctaaacgc gccccaggtg gtatggaaac caaaaatgga gacgggaagc tgaaccagat
    10561 agttactgga ggtgatcacc agcagatgaa ataacgataa ccagaacaac gccttatagc
    10621 gttgagtttg cgagaaaacg ttcatattgt acctttttga ttaaccattg gggtgaggga
    10681 acccaatacg tacgacacgt ctgttacctt aacataataa cgtgcttacc cttttttcga
    10741 gccgccgcat gatactgctt tacgttgaca atgcaatggt taattttcac gcaatcgtta
    10801 acctggtttg cttacggact cacagggcgt aaactagcgc cgttttttta tgtgatgaga
    10861 agaaaatgac cgatctcttt tccagccctg accacacact cgacgcactt ggcctgcgct
    10921 gcccggaacc cgtaatgatg gtgcgcaaaa ctgtgcgcaa tatgcagccc ggcgaaacgt
    10981 tgctgattat cgccgacgat ccggccacta cccgcgatat tcctgggttt tgtaccttta
    11041 tggaacacga actggttgcg aaagagacgg atggactgcc ttatcgttat ttgattcgta
    11101 aaggcggttg atgggggctg attggcttcg atgccgcctt ttcccctcac cctaaccctc
    11161 tccccagagg ggcgagggga ccgatcgcgc tcaatgttgc gatcggtttg ccttatctcc
    11221 tgcgcaacaa tcttaacgca ttcgctgtca ccagcaccgt cgcacccgta tctgccagca
    11281 ccgccagcca tagtccggtc atccctaaca gcgtggtgac gaggaagatc cctttcagtc
    11341 ccagcgcaat ggtgatgttc tggcggatat tggcgtgagt ggcgcgtgcc agttcaatca
    11401 tttgcaccag gccgcgcagg tggttatggg ttaatgctgc atcggcggtt tccagcgcca
    11461 cgtctgtgcc gctgcccatt gcaatcccaa tggcggcagc tttcatcgct ggcgcgtcgt
    11521 taataccatc accgaccatc gccagcggcg catgttgatt cagcttggtc accgctttga
    11581 ctttctcttc cggcaatagg cccgctttaa actccagccc cagctccccg gcgattgccg
    11641 ccgctgcgcg tggattatcg ccggtgagga tcactccttt aactcccagc gcgttcagtt
    11701 cactgatggc agttgcagcg tcggcgcgca gggtatcctg caatgcaatg atacccagca
    11761 cgtcatcatt acgtactacc agcacaaccg tttgcccggc gctttccagt tcattaatca
    11821 aaccagcaaa tgcatcagcg ggatgtttcc cggcagcgca aatcaatacg cgctcaccgt
    11881 taacctgcgc ttcaatgcca gacccgacca gcgcccgctg tgattcggcg gtgggaatgg
    11941 cgagttcagc aacctgtgct tctcgcacga tggcctgcgc cagtggatgc gtcgcacctt
    12001 gctcgaccgc cgctgccagt gtcagcagtt cagattcact aatacccgtt gccggatgaa
    12061 tcgcggtaac gcgcggttta ccgacggtca gcgtaccggt tttatcaaac gccacctggg
    12121 taacacgacc tagttgttcc agcgccgctc cgcctttaat taacgcccca cgacgcgctg
    12181 ctgccgccag cccggaggta atcgccgcgg gcgtggagat aactaacgca cacgggcagc
    12241 caatcagcag cagcgtcagg cctttataaa tccactcttg ccagctggcg gcaaacagca
    12301 gcggtggcac cagcgtcacc agcagagcga cggccataat cgcgggcgta tagatacggc
    12361 tgaaacggtc gataaaccgc tcaatgggtg cgcgacgctc ttcggcttct tcgatcagtt
    12421 tcagaatccg gtcaatggcg ctggcacccg gttctgacag cacttccagc gttaccagac
    12481 ggtctacgct ggtggctcca gcagggactt tatcgcccgt tgcgcgctcc accggaatag
    12541 attcgccggt cagggcgctt tcatcaaaac tggcaaacgg tgagagcagt ttaccgtcgg
    12601 caggcaaacg tccacctgcg gcgacttcaa tcacatcgcc agggcgcagg ctgttaatcg
    12661 ccacctcttc ccgctcaccg ttacgcaggc gcgtggcggt ttctggtttc agcgccatta
    12721 acgcgctgac cccttgacgt gcgcggctgg cggcccagcc ttccagtcgt tcgccaatca
    12781 aaaacagcag caacaccatc gcagcttcag ccgttgcgcc aataaaaagc gcaccaatag
    12841 cggctacgct cattaaggtt tcaatggcga agtagctgcc ggatttgatc aaccgtaatg
    12901 cctgacgagc aatcgggtac agcccaacca gtgtggtcgc gataaacgcc agttgcccga
    12961 acggatgatt gaactgctcc agaccccagc tgattgccat catcacgatt agcgtaatca
    13021 gcggcagatt ctctttcagg cgtgatgctt gcggttcgtc ggcggactgt tcatcgcgca
    13081 gggagtagcc cgctttttgc accgcagatt caacctgggc acggatgtca ttgtcggcat
    13141 caaccaccag tttttcggtg gcgaacaaca cctgtacctg attcacgcct gcaagctggc
    13201 gcacggcatt ttctaccttg cgcgcacagg cggcgcagtc catgccgctg actttccagc
    13261 tatagcgggt gccggagacg ttttcagaga gagttggcgt gctggaacat gcgccgtcgc
    13321 agcaacagtc gttggcgttc tgtaccgtgg ttagcggttt gaacgcagca aattgagggg
    13381 ctttcttgcc gtgattgtca ggagtcgaca tggcatcctc cggttaagtt ttttctcatt
    13441 aaccgaagga tacactctgg agtcgactcc agagtcaagt tttatcagag atacagcgag
    13501 cggacgatca ggaagtgacc ggcaaagtag caggccgcag caatcgcgtt atccgcacgg
    13561 aagcgtcggc gatagtgact acccagccag acaaagttac tgataaacag caacgaagcg
    13621 ccgacaaacg cagagagcgc cggagcggtc ggacggaaga accacagttc acctgccagc
    13681 cacaccatca ccagcgtcat gccgataaac gtgcagatag gccaacgata ctcttccagc
    13741 cgcgtccaga taatcgccag taacagcgcg cccagaacca gcagcaccag tggcagaggc
    13801 cagaagaaag agagcgtcat ctgactggcg aaatagatgg tgtacagcag gtgcgagagg
    13861 aaaaacgcgc cgatggcgta catcagacgt tgacgtggca acagggttag cgcatcgccc
    13921 agcagtgagg cgcacagccc cgccagcacc agatagctaa tggcgtcgaa catcggcgct
    13981 tgccaggcca gtaacagcag gagaagaagg gttaatggtt taaataccca gcgttgccag
    14041 gttggcccac gatacgatgc atccacagat agccatgcgg aaagacagac agcgataaac
    14101 gaccaaagca tcttagttcc ttgaattcgt tattttttcg taagcggaat gcctacggtc
    14161 ttgcttccag tttagtggcg agggtggacg gatgacaaca ccataactgg cagggtatgc
    14221 ttaattccgc attttccgat gagggataaa gatgagcaaa ccacctcttt tctttattgt
    14281 tatcattggc ttaatcatcg tcgccgcatc gtttcgtttt atgcaacaac gacgggaaaa
    14341 agctgataat gatatggcac cactccagca aaagctggtg ttggtgagca acaagcggga
    14401 aaaaccgatt aacgatcgcc gttcgcgcca gcaggaagtg actccggcag gcaccagtat
    14461 gcgctatgag gcaagcttca aaccgcaaag cggaggaatg gagcagacgt ttcgcctcga
    14521 cgcccagcag taccacgccc tgacagtggg cgataaaggt acgctgagct ataaaggaac
    14581 gcgctttgtc agctttgtag gcgaacaata aggttatttt ttaactttaa atttcttctg
    14641 ccagaccagc agttcaaaca cgccgaaaag gaaaatccgt accttttcgg cggtggtcat
    14701 ctgcgggcca tctttcggta aagtggattt caatagcgcc agctgcatac catgcatcag
    14761 cacggtaaaa atcagcgcca cgttaacgaa aatattcagc ggacgtggga agggatgcac
    14821 caggttgagg attaaaaatc cccaaacaca gagcattaac aaacgaccaa tattaatcag
    14881 catcactttc tccttgtgct tcgcgttgat acagccgata agccacctga cccgccactt
    14941 tttcccgatg taatgaccag tttgccggaa cagtgggcag accgttttcg acttcgcttt
    15001 cgacataaat cagggcttcg tcagccagcc agccgttatc ttccagtaaa tttatcgtct
    15061 cttctaacaa gccacggcgg aacggtggat cgacaaacac gatattatgc ggtgtacctt
    15121 tttgcgccag gaatgacatc gcgttgctgt tcaccacgcg tgcattgcct gcttttagtg
    15181 tcgccagatt cttaattaac tgctgagaaa ccgcgcgatc catctcaatc aacgttgccc
    15241 ccgcagcata gcgcgacaac gcttccagcc ccagcgcgcc gcttccggcg aagcaatcca
    15301 gacattgggc gtcaacaatg accggagcca gccagttaaa caacgtttcg cgtacgcggt
    15361 cggtggtggg gcgcagacct gggctatcag gaaccgggag tttacggcct cgccactgcc
    15421 cgccaataat gcggatttgg ccgctgccgg aatgattcgg ttttttcatg ataaattgct
    15481 caatccgcca gataacacat acttgcaggc ggtgatgtga attaagttaa gtgatagact
    15541 atttcatcat ttttttagct gctatgtaca tagcgttaac gctgtgccat gaagcaacag
    15601 cgaggagtgt agtcgcaaat ggcgaaagaa aaaaaacgtg gctttttttc ctggctgggc
    15661 tttggtcaaa aagagcagac cccggaaaaa gagacagaag ttcagaatga acaaccggtt
    15721 gtagaagaaa tcgttcaggc gcaagagcct gtgaaggctt cggaacaagc cgttgaagag
    15781 cagccgcagg cgcatactga agccgaggcg gaaacttttg ctgccgacgt tgtggaagtc
    15841 actgaacagg ttgctgaaag tgaaaaagcg cagcctgaag cggaagtcgt tgcacagccg
    15901 gaaccggtcg cagaagaaac gccggagcca gtggctatcg aacgtgaaga gctgccgttg
    15961 ccagaagacg tcaacgccga agaggtttcg ccagaagagt ggcaggctga agcggaaacc
    16021 gtagagattg tcgaagcggc agaagaagaa gcggctaaag aagaaatcac cgacgaagag
    16081 ctggaagcac aggcgctggc tgccgaagcg gcagaagagg cggtgatggt agttcctccg
    16141 gcagaagaag agcagccggt ggaagaaatt gcccaggagc aagaaaaacc gaccaaagaa
    16201 ggtttcttcg cgcgcctgaa acgcagcctg ttaaaaacca aagaaaatct cggttccgga
    16261 tttatcagcc tgttccgcgg taaaaaaatc gacgatgatc tgtttgagga gctggaagaa
    16321 cagctgttga tcgccgatgt gggcgtggaa accacacgta aaattatcac caatctgacg
    16381 gaaggcgcat cccgcaagca gcttcgtgat gccgaggcgc tctatggcct gctgaaagaa
    16441 gagatgggcg agattctggc gaaagtcgat gagccgctga atgttgaagg taaaacgccg
    16501 ttcgtgatcc tgatggtggg cgtcaacggt gtgggtaaaa ctacgacgat tggtaagctg
    16561 gcacgtcagt ttgagcagca gggtaaatcg gtgatgctgg cggcgggcga tactttccgt
    16621 gcagcagcgg ttgaacagct tcaggtctgg ggtcagcgca acaatattcc ggtgattgcc
    16681 cagcataccg gtgcggattc cgcctctgtt atcttcgacg ccattcaggc agctaaagcg
    16741 cgtaatatcg acgtcctgat tgccgatacc gccggacgcc tgcagaacaa atcgcacctg
    16801 atggaagagt tgaagaaaat cgtccgcgtg atgaagaaac tcgacgttga agcgccgcat
    16861 gaagttatgc tgactattga tgccagtacc gggcagaatg cggtaagcca ggccaaactg
    16921 ttccacgaag ccgttggctt aaccggcatc acgctaacga aactggacgg cacggcgaaa
    16981 ggcggggtaa ttttctcggt ggctgaccag tttggtatcc ctatccgcta cattggtgtc
    17041 ggcgaacgta ttgaggattt gcgtccgttt aaggcggacg actttataga ggcacttttt
    17101 gcccgagagg attaacaatg attcgctttg aacatgtcag caaggcttat ctcggtggga
    17161 gacaggcgct gcagggcgtt acgttccata tgcagccggg tgagatggcg tttctgaccg
    17221 gtcattccgg cgcagggaaa agtaccctcc tgaagctgat ctgtgggatt gagcggccca
    17281 gcgccgggaa aatctggttt agcggccatg acatcacgcg tctgaaaaac cgtgaagttc
    17341 cgtttctgcg ccgccagatt ggcatgattt tccaggatca ccatctactg atggaccgta
    17401 ctgtctacga taacgtggcg atcccgctga ttatcgctgg tgccagcggt gacgatattc
    17461 gtcgccgggt gtcggcggca ctggataaag tcgggctact ggacaaagcg aagaacttcc
    17521 ctattcagct ttccggcggt gaacaacagc gtgttggcat tgcccgcgcg gtggtgaata
    17581 agcccgcggt actgctggcg gacgaaccga ccggtaacct ggacgacgcg ttgtcggaag
    17641 ggattctacg tctgtttgaa gagtttaacc gcgttggggt aaccgtattg atggcaacgc
    17701 acgacatcaa cctgatttca cggcgttcct atcgcatgct caccctgagc gatggtcact
    17761 tgcatggagg cgtgggccat gaataagcgc gatgcaatca atcatattcg gcagtttggc
    17821 gggcgtcttg atcgcttccg taaatcggtc ggcggctctg gcgacggcgg tcgtaacgcc
    17881 ccgaaacgcg cgaaatcctc gccaaaaccg gtaaatcgta aaaccaacgt tttcaacgaa
    17941 caggtgcgct atgccttcca cggcgcattg caggatctga aaagcaaacc gttcgccacg
    18001 tttttaacgg tgatggttat cgccatctcc ctgacgctgc ccagcgtctg ttatatggtg
    18061 tacaaaaacg ttaatcaggc ggcgacgcag tattatccgt caccgcaaat cactgtttac
    18121 ttgcaaaaaa cgctggacga tgacgccgct gcgggcgtgg tggcacagtt gcaggccgag
    18181 caaggcgtgg agaaagtgaa ctatctttct cgtgaagacg cactgggcga gttccgtaac
    18241 tggtctggtt ttggtggtgc gctggatatg ctggaagaaa acccgcttcc ggcagtggcg
    18301 gtggtgatcc cgaaactcga ttttcagggg acggaatcac tcaatacgct gcgtgatcgt
    18361 atcacgcaga ttaacggcat tgacgaagtg cggatggatg acagctggtt tgcccgtctg
    18421 gcggcgttga ccgggctggt cgggcgcgtt tcggcgatga tcggcgtgtt gatggtggcg
    18481 gcagtgttcc tcgtcatcgg caacagcgta cgtctgagca tctttgctcg ccgtgactcc
    18541 atcaacgtgc agaaactgat tggtgcgacg gatggtttca tccttcgccc gttcctgtat
    18601 ggtggcgcac tgctgggatt ttctggcgca ttgttgtcat taattttgtc agaaattctg
    18661 atgctgcgat tgtcatcggc ggttgcggaa gtggcacagg ttttcggaac gaagtttgat
    18721 atcaatggtt tatcgttcga tgaatgcctg ctattgctgc tggtatgctc gatgattggc
    18781 tgggtggcag cgtggcttgc cacggtacaa catttacgcc actttacgcc tgaataataa
    18841 aagcgtgtta tactctttcc ctgcaatggg ttccgtagca gggaaagaga tcccgttgtc
    18901 tcttcccggt atttcatctc tatgtcacat tttgtgcgta atttattcac aagcttgcat
    18961 tgaacttgtg gataaaatca cggtctgata aaacagtgaa tgataacctc gttgctctta
    19021 agctctggca cagttgttgc taccactgaa gcgccagaag atatcgattg agaggatttg
    19081 aatgactgac aaaatgcaaa gtttagcttt agccccagtt ggcaacctgg attcctacat
    19141 ccgggcagct aacgcgtggc cgatgttgtc ggctgacgag gagcgggcgc tggctgaaaa
    19201 gctgcattac catggcgatc tggaagcagc taaaacgctg atcctgtctc acctgcggtt
    19261 tgttgttcat attgctcgta attatgcggg ctatggcctg ccacaggcgg atttgattca
    19321 ggaaggtaac atcggcctga tgaaagcagt gcgccgtttc aacccggaag tgggtgtgcg
    19381 cctggtctcc ttcgccgttc actggatcaa agcagagatc cacgaatacg ttctgcgtaa
    19441 ctggcgtatc gtcaaagttg cgaccaccaa agcgcagcgc aaactgttct tcaacctgcg
    19501 taaaaccaag cagcgtctgg gctggtttaa ccaggatgaa gtcgaaatgg tggcccgtga
    19561 actgggcgta accagcaaag acgttcgtga gatggaatca cgtatggcgg cacaggacat
    19621 gacctttgac ctgtcttccg acgacgattc cgacagccag ccgatggcac cggtgctcta
    19681 tctgcaggat aaatcatcta actttgccga cggcatcgaa gatgataact gggaagagca
    19741 ggcggcaaac cgtctgaccg acgcgatgca gggtctggac gaacgcagcc aggacatcat
    19801 ccgtgcgcgc tggctggacg aagacaacaa gtccacgttg caggaactgg ctgaccgtta
    19861 cggtgtttcc gctgaacgtg tgcgccaact ggaaaagaac gcgatgaaaa aacttcgcgc
    19921 cgctattgaa gcgtaatttc cgctattaag cagagaaccc tggatgagag tccggggttt
    19981 ttgttttttg ggcctctaca ataatcaatt ccccctccgg caaaacgtca atccccacgc
    20041 agattgttaa taaactgtca aaatatctat tccaatatca taaaaatggg gtatgtttta
    20101 gcagagtatg ctgctaaagc acgggtagtc atgcataaaa cgaaataaag tgctgaatca
    20161 caacaccaca acacacgtaa taaccagaag aatggggatt ctcaggatga atataaaggg
    20221 taaagcgtta ctggcaggat gtatcgcgct ggcattcagc aatatggctc tggcagaaga
    20281 tattaaagtc gcggtcgtgg gcgcaatgtc cggtccggtt gcgcagtacg gtgaccagga
    20341 gtttaccggc gcagagcagg cggttgcgga tatcaacgct aaaggcggca ttaaaggcaa
    20401 caaactgcaa atcgtaaaat atgacgatgc ctgtgacccg aaacaggcgg ttgcggtggc
    20461 gaacaaagtc gttaacgacg gcattaaata tgtgattggt cacctctgtt cttcatcaac
    20521 gcagcctgcg tctgatatct acgaagacga aggcatttta atgatcaccc cagcggcaac
    20581 cgcgccggag ctgaccgccc gtggctatca gctgatcctg cgcaccaccg gcctggactc
    20641 cgaccagggg ccgacggcgg cgaaatatat tcttgagaaa gtgaaaccgc agcgtattgc
    20701 tatcgttcac gacaaacagc aatacggcga aggtctggca cgagcggtgc aggacggcct
    20761 gaagaaaggc aatgctaacg tggtgttctt tgatggtatc accgccgggg aaaaagattt
    20821 ctctacgctg gtggcgcgtc tgaagaaaga gaatatcgac ttcgtttact acggcggtta
    20881 ccacccggaa atggggcaaa tcctgcgtca ggcacgcgcg gcagggctga aaactcagtt
    20941 tatggggccg gaaggtgtgg ctaacgtttc gctgtctaac attgcgggcg aatcagcgga
    21001 agggctgctg gtgaccaagc cgaagaacta cgatcaggtt ccggcaaaca aacccattgt
    21061 tgacgcgatc aaagcgaaaa aacaggaccc aagcggcgca ttcgtttgga ccacctacgc
    21121 cgcgctgcaa tctttgcagg cgggcctgaa tcagtctgac gatccggctg agatcgccaa
    21181 atacctgaaa gcgaactccg tggataccgt aatgggaccg ctgacgtggg atgagaaagg
    21241 cgatctgaaa ggctttgagt tcggcgtatt tgactggcac gccaacggca cggcgaccga
    21301 tgcgaagtaa tcattaatcg gcaactttgg gttgccgcca aattgctaat atcgagtacg
    21361 ttgcttcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaagatcc aaagaaatca
    21421 gtaaattgca acacacattg taggcctgat aagcgtagcg catcaggcaa tttctcgttg
    21481 gtcatcactt ttgaaatcaa cttgacgatt aacgcttctc ccagccgccc tgttgcgccg
    21541 taaaccccag cgcctgcata aacgccgtca tcacaccgcg atcttccaag cctgcatccg
    21601 ccatccacca gcatgaaacg ccaggattgt tacgcaaaac ctcttccagc agatattgcc
    21661 ccacaccgcg acgtcgggtg acttcccgca cacgcaggga atccagtgct ccctcggtgc
    21721 cgcttaaggt tacccgtacg gcagcgagca ggcgctcgtt aaaacgcgcg gcgtagatac
    21781 ggtggttatc gtcaacctgt aaagaggaag gggaatactc cggccagatc ttttgcaggt
    21841 caatccggtc ctggtcgcta aagttttcta atcgaatgat ggtcagcttc atgggtaacc
    21901 cgtgtaaatc aca
//
LOCUS       DAZFRJ010000064        21871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000064 DAZFRJ010000000
VERSION     DAZFRJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.064"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..331
                     /gene="mokC"
                     /locus_tag="ACHKUN_002239"
     CDS             179..331
                     /gene="mokC"
                     /locus_tag="ACHKUN_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO6660490.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(435..1565)
                     /gene="dnaJ"
                     /locus_tag="ACHKUN_002240"
     CDS             complement(435..1565)
                     /gene="dnaJ"
                     /locus_tag="ACHKUN_002240"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO6660491.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1654..3570)
                     /gene="dnaK"
                     /locus_tag="ACHKUN_002241"
     CDS             complement(1654..3570)
                     /gene="dnaK"
                     /locus_tag="ACHKUN_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO6660492.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3947..4351
                     /gene="yaaI"
                     /locus_tag="ACHKUN_002242"
     CDS             3947..4351
                     /gene="yaaI"
                     /locus_tag="ACHKUN_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO6660493.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4377..5090
                     /gene="msyB"
                     /locus_tag="ACHKUN_002243"
     CDS             4377..5090
                     /gene="msyB"
                     /locus_tag="ACHKUN_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6660494.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELAELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5239..5805
                     /gene="satP"
                     /locus_tag="ACHKUN_002244"
     CDS             5239..5805
                     /gene="satP"
                     /locus_tag="ACHKUN_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO6660495.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5840..6427)
                     /gene="mog"
                     /locus_tag="ACHKUN_002245"
     CDS             complement(5840..6427)
                     /gene="mog"
                     /locus_tag="ACHKUN_002245"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO6660496.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6542..7495)
                     /gene="tal"
                     /locus_tag="ACHKUN_002246"
     CDS             complement(6542..7495)
                     /gene="tal"
                     /locus_tag="ACHKUN_002246"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6660497.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7774..9204
                     /gene="yaaJ"
                     /locus_tag="ACHKUN_002247"
     CDS             7774..9204
                     /gene="yaaJ"
                     /locus_tag="ACHKUN_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO6660498.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9274..10050
                     /gene="yaaA"
                     /locus_tag="ACHKUN_002248"
     CDS             9274..10050
                     /gene="yaaA"
                     /locus_tag="ACHKUN_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO6660499.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10203..10499)
                     /gene="yaaX"
                     /locus_tag="ACHKUN_002249"
     CDS             complement(10203..10499)
                     /gene="yaaX"
                     /locus_tag="ACHKUN_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO6660500.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10713..11999)
                     /gene="thrC"
                     /locus_tag="ACHKUN_002250"
     CDS             complement(10713..11999)
                     /gene="thrC"
                     /locus_tag="ACHKUN_002250"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO6660501.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12000..12932)
                     /gene="thrB"
                     /locus_tag="ACHKUN_002251"
     CDS             complement(12000..12932)
                     /gene="thrB"
                     /locus_tag="ACHKUN_002251"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO6660502.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12934..15396)
                     /gene="thrA"
                     /locus_tag="ACHKUN_002252"
     CDS             complement(12934..15396)
                     /gene="thrA"
                     /locus_tag="ACHKUN_002252"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO6660503.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15423..15542)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15478..15543)
                     /gene="thrL"
                     /locus_tag="ACHKUN_002253"
     CDS             complement(15478..15543)
                     /gene="thrL"
                     /locus_tag="ACHKUN_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO6660504.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(15757..16443)
                     /gene="yjtD"
                     /locus_tag="ACHKUN_002254"
     CDS             complement(15757..16443)
                     /gene="yjtD"
                     /locus_tag="ACHKUN_002254"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6660505.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16843..16983)
                     /gene="yjjY"
                     /locus_tag="ACHKUN_002255"
     CDS             complement(16843..16983)
                     /gene="yjjY"
                     /locus_tag="ACHKUN_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO6660506.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17079..17795
                     /gene="arcA"
                     /locus_tag="ACHKUN_002256"
     CDS             17079..17795
                     /gene="arcA"
                     /locus_tag="ACHKUN_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO6660507.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17855..19207)
                     /gene="creD"
                     /locus_tag="ACHKUN_002257"
     CDS             complement(17855..19207)
                     /gene="creD"
                     /locus_tag="ACHKUN_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO6660508.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(19265..20689)
                     /gene="creC"
                     /locus_tag="ACHKUN_002258"
     CDS             complement(19265..20689)
                     /gene="creC"
                     /locus_tag="ACHKUN_002258"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO6660509.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20689..21378)
                     /gene="creB"
                     /locus_tag="ACHKUN_002259"
     CDS             complement(20689..21378)
                     /gene="creB"
                     /locus_tag="ACHKUN_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO6660510.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21391..21864)
                     /gene="creA"
                     /locus_tag="ACHKUN_002260"
     CDS             complement(21391..21864)
                     /gene="creA"
                     /locus_tag="ACHKUN_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO6660511.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
BASE COUNT         5207 a         5858 c         5351 g         5455 t
ORIGIN      
        1 gggttaaaga gagcgatata atagcggcgg gtacttgagg ctatctgtct caggcattag
       61 ctgaacggca gatagagaaa agccccgagt gatattttac tatcaacccg aggcccccta
      121 tatgctgaac acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat
      181 gaagcagcat aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc
      241 ggcgctggta acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt
      301 tgctgttttc acggcttacg aatccgagta agtgcaacgg cggggagtga tccccgccac
      361 ttttcaggtg ctcgcatatc ttcaacgcac cctaatttta cccaagcctg cccacgggca
      421 ggcttttggg gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag
      481 ctctttgagc gcgggctgtt gtgctcaccg gttgggccac cgaagctttc ttgcagctct
      541 tgcagcagct gtttctgctt ctcgttcagg cctaccggtg tttcgacgac aacgcggcac
      601 agcaaatcac cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaaa
      661 agcttaccgg tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt
      721 acttcgattt cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatagagg
      781 ttgttgcctt cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg
      841 cctgccggtg cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca
      901 gtgtccaccc ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga
      961 ccatgacatt tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag
     1021 gtctgctgta cagcgaagaa tccctgacgc atctgcacct gaccagaacc atgacaggtc
     1081 ggacaggtct gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct
     1141 tccagagtcg gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc
     1201 tccatgttat agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca
     1261 aaaatatcgc cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaaccg
     1321 ccgccgccca taccaccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt
     1381 ttttgcgagt cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc
     1441 tctttgtcac cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt
     1501 ttgatttcac gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta
     1561 gccatctttt ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc
     1621 ccgtgtcagt ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa
     1681 ttcagcgtcg acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt
     1741 ctgctgctgg gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag
     1801 ctcctgcatt ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc
     1861 agtcagtgca gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc
     1921 aacctgctta cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc
     1981 ttcaaactta cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc
     2041 atcttcgttc agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt
     2101 atctttcgcg gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg
     2161 cggcatgccg cgcggtgccg ggttgatacc atccaggttg aactgaccca gagatttgtt
     2221 atcagccgca cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc
     2281 ttcagcggta gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag
     2341 cgtcgtcatc acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag
     2401 cagtacgtct ttcacgtcac cagtcagaac accaccctga acagcagcac cgattgctac
     2461 agcttcgtcc gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt
     2521 ctgaaccatt ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac
     2581 ggacaggcca gcgtcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc
     2641 ttcaaccagg ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt
     2701 cgcatctgca gtgatgtacg gcaggttaac gtcggtctgc tgagcggaag acagttcgat
     2761 tttcgctttt tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc
     2821 aatgccctga tctttcttga attcttcaac caggtagttg atcagacggc tgtcgaagtc
     2881 ttcaccaccc aggtgggtat caccgttggt agccagaact tcgaaggttt tttcgccgtc
     2941 aacttcgtcg atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc
     3001 gatagtacgg ttgccagtac ctttgtccag accgtaagcc agcgcagctg cggtcggttc
     3061 gttgatgata cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg
     3121 ctgagcatcg ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccaa
     3181 gtaatcttca gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc
     3241 cattttctgg cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa
     3301 cggcatgatg gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg
     3361 tttaatcgca aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc
     3421 aaccagagtt tcaccatcct gggtataggc aataatagaa ggcgtggtgc gatcgccttc
     3481 ggcgttctcc agcacgcgag gagtggtgcc atccataatc gctacacaag agttggtagt
     3541 acccaggtcg ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc
     3601 atgtggttgt gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt
     3661 ttttgcagac tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag
     3721 ggggagatgc aaaaattttt tgtgcttttg cgcagaaatt tcgcgatata gcaggacaag
     3781 acgtgaaact gacagcaatt ttattcatat aaagaatgaa tcgcagccaa gacattcatc
     3841 aaaaaattgt aataaaaaga aaacattacg cacctgaatc ttctctttat cagcagtaaa
     3901 ctagtgggta tccatccccc tacctctccc cactaagaga atccttatga aatccgtttt
     3961 tacgatttcc gccagcctgg cgattagtct gatgctgtgc tgcacggcgc aggcaaacga
     4021 ccataaaatc ctcggcgtca ttgcgatgcc acgtaacgaa accaacgatc tggcgctgaa
     4081 acttcctgtt tgccgaattg ttaaacgcat acaactctct gccgaccatg gcgatttaca
     4141 gttaagcggt gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat
     4201 tccttcagaa ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga
     4261 caataaacgc tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat
     4321 ggccacgctg aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga
     4381 atgttaatta cctgaatgat tcagatctgg attttctcca gcattgcagt gaggaacagt
     4441 tggcaaattt cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg
     4501 tactgatgcg caacgaactg tttaaatcta tggaagggca tcccgagcaa catcgccgca
     4561 actggcagct gattgctgga gaattacagc attttggtgg cgatagtatc gccaacaaac
     4621 tgcgcggaca cggtaaattg tatcgggcca ttttgctcga tgtttcaaaa cgattgaagc
     4681 tgaaagccga caaagagatg tctacgtttg agattgagca acagttactg gaacaatttc
     4741 tgcgtaacac ctggaagaat atggacgagg aacataaaca ggagtttctg cacgcggtcg
     4801 atgcgagggt gaatgagctg gcagagctgc tgccgctgct gatgaaagac aaattattgg
     4861 caaaaggcgt gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag
     4921 caatgagcgt acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtaggtg
     4981 cggcactaaa tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg
     5041 tactgcaaat cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac
     5101 cggggaaaat tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat
     5161 tatgccgcgc cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat
     5221 gatgattttt gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg
     5281 atgggcttcg gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg
     5341 gacggtatta ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgccggt
     5401 ctgctggagt acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct
     5461 ttctggctga cgttggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca
     5521 aatgcacagt tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc
     5581 ttcggcacgc tgaaaggcgc acgcgttctg caattcgttt tctttagcct gactgtgctg
     5641 tttgccctgc tggcgatcgg taacattgcc ggtaacgccg caatcatcca ctttgccggc
     5701 tggattggtc tgatctgtgg tgccagtgca atctatctgg cgatgggtga agtactgaac
     5761 gagcagtttg gtcgcaccgt tctgccgatt ggtgaatccc actaattgtt ttgagatccc
     5821 cccgctcggg gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg
     5881 cagcaaccac ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg
     5941 gtacgctggc aaaaataccg tgtaccacaa cgttacccgc agcgtccttc acaccttcca
     6001 gcgtctcttt aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa
     6061 tcacaccaac ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctt atctggcgca
     6121 tctgttcacc aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta
     6181 cgtcacgacg cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat
     6241 ccactagctc acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt
     6301 ccagttcaaa cggcgtggtt agcgccgatg tcagccattc ttccagcgca gggatgcctt
     6361 tatcctgata aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag
     6421 tattcatcaa gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg
     6481 gagtgacaga caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg
     6541 attacagcag atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga
     6601 taccttccgc cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg
     6661 actcagtgat acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga
     6721 tagccccttc gctctcagcc agctctttca gcagtgtagg tgcgatggta agacggtcgc
     6781 agcctgccag ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt
     6841 cataaccgtg ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt
     6901 ctgccggagc gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa
     6961 caaacggcga gatcaggaac acgcccgctt ccgcacaagc acgagcctga gcgaaggaga
     7021 acagcagggt caggttacag ttgatgcctt ctttttccag ctgttctgca gcacggatac
     7081 cctgccaggt agaagccagt ttgatcagaa tacgatcgtt gctaatacca gcatcgttgt
     7141 agagtttgat caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg
     7201 catcaacttc agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta
     7261 ctgccagttt gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg
     7321 cccaggcgac agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa
     7381 tgagagaagg gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc
     7441 cagtgtcggc cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag
     7501 tatttctctt taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga
     7561 cgcaacgtca gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt
     7621 ctgacactgc gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga
     7681 agttatcaag tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga
     7741 aagtcatcgc gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac
     7801 agcgtccttt ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact
     7861 tttcgcaccg gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat
     7921 agcattcatc cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg
     7981 gcgcgcgtgg gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacct
     8041 ggagccgtct tctggatgtg ggttgccgcg tttatcggca tggcgacctc gtttgccgaa
     8101 tgttccctcg cacagcttta taaagaacgt gatgccaatg ggcagtttcg tggcggaccg
     8161 gcatggtata tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt
     8221 ttgctcatcg cctacggcat aattttcagc ggaattcagg cgaacgccgt tgcgcgagcc
     8281 ctgagttttt cttttgattt tcccccgctg gtgacaggta ttattctcgc tgtctttgct
     8341 ctgctggcga tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg
     8401 ttgatggcga taatctgggt actgaccagc ctggtgattt gcgtaatgaa tatcgggcaa
     8461 cttccccacg tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc
     8521 ggcgcggcgg gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt
     8581 tccaatgagg cgggaatggg gtcgacgcca aacgcggcag cggcagcggc gtcctggcct
     8641 ccgcatccgg cagcgcaagg gattgtccag atgattggca tttttatcga caccctggtc
     8701 atctgtacgg caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg
     8761 ctggaaggta ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctggggtgct
     8821 gagtttgtta ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt
     8881 tacgccgaaa acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg
     8941 cggatctgca ccttcgcaac ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg
     9001 caactggcag atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg
     9061 ctctcgcctg tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg
     9121 cgcccggtgt ttgatccgtt gcgttatccg gatatcggcc gccagctttc tccggacgcg
     9181 tgggatgatg tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct
     9241 aaagtcggca taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa
     9301 acgcttgatt accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac
     9361 aattcccagc agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg
     9421 atgcgcatca gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca
     9481 gatttcacgc cggagaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc
     9541 ggcttgcagg ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga
     9601 atgctttccg gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg
     9661 gaaatgggga tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat
     9721 atcatcacca acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat
     9781 ctggcgtcag atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc
     9841 aagccggtgt tcctcgatga gaagaacggc aaatttaaga taatcagctt ctacgctaag
     9901 aaagcacgcg gtctgatgag tcgttttatt attgaaaatc ggctgaccaa accagagcaa
     9961 ctgactggtt ttaatagcga aggttacttc tttgatgaag cttcctccag caatggcgaa
    10021 ctggtgttta aacgctacga gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa
    10081 cgccttatcc ggcctacaaa agcatgcaga ttcaatatat tgcagaattc acgtaggcct
    10141 gataagacgc gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc
    10201 atttagcggt gatgtttgcc tggaccatga ccgccgtgat gatcatgatg agctttctta
    10261 tggtgacgcg gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa
    10321 tgttgtttcc accagccgtg gtcgcgccag tgaccgccat cccagtaata accgcgatta
    10381 tcacgatcgc ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgcgct
    10441 gccatgggag cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca
    10501 ggttactcct tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac
    10561 ggcggttgtt attctccgca atcctaatct ctaagttacc gcatttattg agaatttctc
    10621 ctttttccct gtcgtttctc tccatatttt cttcacaaaa aagccgggct gcataaaagc
    10681 aaacccggcc tgattgagat aatgaataga tgttactgat gattcatcat caatttacgc
    10741 aacgcagcaa aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc
    10801 agctcttttg gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc
    10861 gccggatgcg cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc
    10921 gcacgatacg ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc
    10981 attgtctgtt gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag
    11041 attttgcggc ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc
    11101 gcgttggata acgtcgcctg agtcgctttg ggggaccact gaccgtcgtg caggaaacgt
    11161 ggcacggtat cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgagtgac
    11221 ttcgccagca gacccgccgt caaatcgccg aagtttccgc ttggtaccga aacaaccagc
    11281 tggttgcgcg cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc
    11341 agcaaacggc tgatgttaat cgagttagct gagtttaacc ccagcgccac tttcagttct
    11401 tcatcatcaa acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca
    11461 acagtttcga tattgccgcc caatgtacag aacagttttt cttgcagtgg actgattttg
    11521 cctcgtggat agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact
    11581 gccgctccgg tatcaccgga ggtcgcggtc agaatggtca ctggcttatc acccgcaata
    11641 tgggtcagca tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg
    11701 tggaacaatt ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca
    11761 aacgccgcgc gcacgcgctc ttccaggatt tcctgcggga tttcatcacc aataaacgcc
    11821 gagaggatct tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc
    11881 aggctgaatt ccggcaggtc atgcggaaaa aacagcccct gatttttgcc caacccctgg
    11941 gttacggctt gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt
    12001 tagttttcca gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc
    12061 tgattttgca ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg
    12121 tcacacagag cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt
    12181 tccgcgaccg cctgccgcgc ctgccggaag cctggcagta atcgttcacg gtagggttca
    12241 gcgataacat ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga
    12301 atgaagcctg ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt
    12361 aaaatagccc gggcttctgc cgtcgagact ttaatccccg gatacgccag cacccacagc
    12421 cactcatcaa acccaggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc
    12481 ataccaccta gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct
    12541 tccagctcgc ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca
    12601 ttcatcgcca tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc
    12661 ggcatattct tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa
    12721 cgctcccagc actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag
    12781 cgcccgaggt tgttgagact gaatatctct gccgcctcaa ccgtaactac atctccgagc
    12841 aatgcaccat caacgggtgt caccgccgcc ccgagcacat caaacccgac gctcatattg
    12901 gcactggaag ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt
    12961 acgcagcaga tcggcaaaga caccagcagc ggtaacgtca ttgcccgcac catatccgcg
    13021 cagcaccaac ggcagcggct gataatagtg gctatagaag gccagggcgt tttcgccatt
    13081 tttcactttg aacagcggat cattaccatc cacttcggca atcttcacgc gacagacgcc
    13141 atcttcatca atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac
    13201 gcgcgcggca aagcgatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc
    13261 ctcggcgtta aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc
    13321 acgtcccgtt tcacgggcga gaatcaatag tttacgcgcc acatccatac cagaaagatc
    13381 atctcgcgga tccggttcgg tataacccat ttcccgtgcc agcgtggtcg cctcggagaa
    13441 actcatgcct tcgtctaact tgccgaagat ataagaaagc gatcctgaaa gaatgccgga
    13501 gaacttcatc aattcatcac cagcattgag cagattttgc aggttttcaa taaccggtaa
    13561 tccagcccca acgttggtgt catagaggaa tttccgccgc gatttttccg ccgcatgacg
    13621 caacagatgg tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg
    13681 gaaaccttcg cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc
    13741 aacaatgacc gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt
    13801 aaacggctct ttggcttgcg ccagttcttc ctgccagttt tccagattta ggccatgcac
    13861 attggtgagc agtgccttcg agttggcaac accgcagaca cgtaagtcga tatgtttatt
    13921 cttcagccag ctttgttgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac
    13981 gccaatcaca aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg
    14041 cacgccagtg gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc
    14101 ctgagcaata gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga
    14161 gatcccacgc aaggtacgca taccatcacc taccaccgag ataatggcca gccgttccgt
    14221 caccgccagc ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc
    14281 ccgttcagcc cgcacacagt cgctttgcgg aacgcagaaa ctgatactgt attcggaaga
    14341 tgattgcgta atcagcacca cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc
    14401 cgccatgccg accattcctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt
    14461 cagattggaa atgcccttga ccggtaattc gtcttcatca cgactggcac caatgagcgt
    14521 acctggtgct tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat
    14581 gggggtaatg gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc
    14641 ctggtaggac atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata
    14701 gaccccgtca acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac
    14761 cgccgcggag tagtcggaac cgttgcgtcc aagtaccacc agttcgcctt tttcattacc
    14821 ggcggtgaag cctgccatca gcaccatgtg atcagccgga atacgacttg ccgcaatacg
    14881 gcgggtggac tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagtagttt
    14941 ttcgaccgga tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat
    15001 ggcgatcgac attttctcgc cacggcaaat cagcgcagcg ttaatgctat ccgggcactg
    15061 ccctaacaaa ctaatgccat gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa
    15121 agttttcaat tgcgccagcg ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc
    15181 ggcaaaaata cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt
    15241 ttcaatcatc gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac
    15301 ctgcccctgc ctggcattgc tttccagaat atcggcaacc cgcagaaaac gttctgcatt
    15361 tgccactgat gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt
    15421 cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
    15481 gcccgcaccg ttacctgtgg taatggtgat ggtggtgata atggtggtgc taatgcgttt
    15541 catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta tattggttaa
    15601 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
    15661 aagctgctgt cagagactct ttttttaatc cacacagaga catattgccc attgcagtca
    15721 gaatgaaaag ctgaaaaata cttactaagg cgttttttat ttggtgatat ttttttcaat
    15781 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
    15841 ctgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca gcaatgccat
    15901 gactcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac ttttcgtcgg
    15961 ttgttgtatt aatgttgcta attgatagca atagaccatc actgcctgcc ccagattgag
    16021 cgaaggataa tccgccacca tcggcacacc agtaagaacg tcagccaacg ctaactcttc
    16081 gttagtcaac ccggaatctt cgcgaccaaa caccagcgcg gcatggctca tccatgaaga
    16141 tttttcctct aacagcggca ccagttcaac tggcgtggcg tagtaatgat atttcgcccg
    16201 actgcgcgca gtggtggcga cagtgaaatc gacatcgtgt aacgattcaa ccagtgtcgg
    16261 gaaaacttta atattatcaa taatatcacc agatccatgt gcgacccagc gagtggctgg
    16321 ctccagatgt gcctgactat cgacaatccg cagttcgcta aaccccatcg ttttcattgc
    16381 ccgcgccgct gccccaatat tttctgctct ggcgggtgcg accagaataa tcgttatacg
    16441 catattgcca ctcttcttga ccaaatgacc gcgaacaggg tgatcactgt caacttatta
    16501 cgcggtgcga atttacaaat ccttaacgta agtcgcagaa aaagcccttt acttagctta
    16561 aaaaaggcta aactatttcc tgactgtact aacggttgaa ttgttaaaaa atgctacata
    16621 tccttctgtt tacttaggat aattttataa aaaataaatc tcgacaattg gattcaccac
    16681 gtttattagt tgtatgatgc aactagttgg attattaaaa taatgtgacg aaagctagca
    16741 tttagatacg atgatttcat caaactgtta acgtgctaca attgaacttg atatatgtca
    16801 acgaagcgta gttttattgg gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat
    16861 ggttaggatg acagccgttt ttgacactgt cgggtcctga gggaaagtac ccacgaccaa
    16921 gctaatgatg ttgttgacgt tgatggaaag tgcatcaaga acgcaattac gtactttagt
    16981 catgttacgc cgatcatgtt aatttgcagc atgcatcagg caggtcaggg acttttgtac
    17041 ttcctgtttc gatttagttg gcaatttagg tagcaaacat gcagaccccg cacattctta
    17101 tcgttgaaga cgagttggta acacgcaaca cgttgaaaag tattttcgaa gcggaaggct
    17161 atgatgtttt cgaagcgaca gatggcgcgg aaatgcatca gatcctctct gaatatgaca
    17221 tcaacctggt gatcatggat atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg
    17281 aactgcgcga gcaggcgaat gttgcgttga tgttcctgac tggccgtgac aacgaagtcg
    17341 ataaaattct cggcctcgaa atcggtgcag atgactacat caccaaaccg ttcaacccgc
    17401 gtgaactgac gattcgtgca cgcaacctgc tgtcccgtac catgaatctg ggtactgtca
    17461 gcgaagaacg tcgtagcgtt gaaagctaca agttcaatgg ttgggaactg gacatcaaca
    17521 gccgttcgtt gatcggccct gatggcgagc agtacaagct gccgcgcagc gagttccgcg
    17581 ccatgcttca cttctgtgaa aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga
    17641 aaatgaccgg ccgtgagctg aaaccacacg accgtactgt agacgtgacg atccgccgta
    17701 ttcgtaaaca tttcgaatct acgccggata cgccggaaat catcgccacc atccacggtg
    17761 aaggttatcg cttctgcggt gatctggaag attaatcggc gttaccaccg tcaaaaaaaa
    17821 tggcgctttt tagcgccgtt tttatttttc aaccttattt ccagatacgt aattgatcgt
    17881 ccattgtaac ttctttactg gctttcattt tcggcagtga aaacgcatac cagtcgatat
    17941 tacgagtcac aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca
    18001 gcgcgctatc ggcagagttg agcagtcccc acatcacacc atccagcaac aacagcgcga
    18061 gggtaaacaa catgctgttg cgccagcctt tcaataccgc ctgcaaataa ataccgttca
    18121 ttaacgcccc aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa
    18181 gcgccagcaa gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg
    18241 ggtgtaaacg ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa
    18301 tcagcagaat ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg
    18361 tcactgcgac gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac
    18421 gctcaccgag attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc
    18481 taacttcccg tttggctggc aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg
    18541 ttaaggtcat ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta
    18601 aattcagggc catattcagc ttcaggttct gcttccgcca gtccccttcg ggtaaaggga
    18661 tatgcacgcc ctgcccgcct tgctctaacc cggtgccggg ttcaatggtc agcgccgttc
    18721 cgttaacttc aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa
    18781 taaagggctt gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga
    18841 aatcggcttt tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc
    18901 gttcttccac gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa
    18961 aacttcgttt ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag
    19021 gaatagcgat aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat
    19081 cttccacatc gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg
    19141 gaatcaacaa cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt
    19201 tcaacatagt cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt
    19261 gaagctatgt gaagtgacgg tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt
    19321 tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca
    19381 gaccgctgct tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg
    19441 acagcgcgta gtcaggaata ccactaccgg tatccagcac cttaagggcg acgtgttcct
    19501 gatccacttc ggcgcttagc gttatgcgac cgctctcggg ggtaaaatcg atggcgttat
    19561 ccagtaaatt ccccagcgcc tgctccagta acgccggttc agcagcaaca ttaacctcag
    19621 tgggcataac atgtagagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga
    19681 cgcggcgaaa taatgccgcc acatcaacaa cagtcagaac gacttcctga cgattctcca
    19741 gtcttgcctg gcgtagtaac gtttctacca atgcctgcat acgcgcattt tgcgtcagaa
    19801 tgttgtcggt aaaacgagcc accacttccg gtggcggacc ttcgcgtaaa atttccgccg
    19861 cgccacgaat cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct
    19921 caatatagtt tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcaagcttac
    19981 gcaactcgct actgccgagt tcaggaagag gaacgggctt attgtcagtg acggaatcgg
    20041 catagcgagt gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca
    20101 ccagtgcaat ccccagcaaa atggcgctgg cccataaaat tcgccgctcg ctacgcttaa
    20161 tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc
    20221 cgtccataat cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca
    20281 acgtgctgcg cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat
    20341 aatcctgccc aacggcttta tttgccgaat cgaacaatac tttaccctgc gaatcggtca
    20401 tatagacatg atattcattg cgcactttgt taatgccacc gatattggcg cgaaacgggc
    20461 gatgttgtag ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca
    20521 aatcgggacg cgccagctcc gccagcaacg ttgcggtatc gattaacgtc ccctccgttg
    20581 ctcttcgcac gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca
    20641 ccgccaccag taaaaaatag cccagcagca accgcatgcc gatacgcatt acaggcccct
    20701 caggctatat cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg
    20761 cagcttggca cgcaacgttt taatgtgggt atcaatggtg cgatcgtagg tgtcctgcgc
    20821 atcttcccac acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag
    20881 taacgtcttc agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct
    20941 gatctgcgcc gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcga
    21001 cgagaacttc ttcacccgac gcagtaaggt gcgcaccctt gcgcacactt cgcggggtga
    21061 aaacggttta gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc
    21121 ttcacttcgg gccgtcagga acagtacagg taacgccgga tggagcgcca gtaactggcg
    21181 gcataattca aagccgctga tatcaggcaa accaacatcg agaatcatga cgtcgggagc
    21241 ctgctggcga gctttatcca gcaccggcaa gcctcgctca aagacctcga cggcaaaacc
    21301 ttcctgctgc aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca
    21361 gaccgtttcc cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc
    21421 gctaatcgcg tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag
    21481 cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt
    21541 gcgttttttg aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc
    21601 aatcggcccg acttgctgac aagaaatagc cgcatcggag gtatcttccg ccagacccaa
    21661 tcccccttta atacccccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc
    21721 gggatcgtca aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt
    21781 gtcgaccgaa ccaatctcct ctgcatgagc caatggcccc agcattatca ggcttaaaga
    21841 caggatcaaa tgcttgtatt tcatattgtt a
//
LOCUS       DAZFRJ010000065        21638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000065 DAZFRJ010000000
VERSION     DAZFRJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.065"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..307
                     /gene="cybC"
                     /locus_tag="ACHKUN_002261"
     CDS             <1..307
                     /gene="cybC"
                     /locus_tag="ACHKUN_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6660512.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHKUN_002262"
     CDS             complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHKUN_002262"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO6660513.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHKUN_002263"
     CDS             complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHKUN_002263"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO6660514.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHKUN_002264"
     CDS             complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHKUN_002264"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO6660515.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHKUN_002265"
     CDS             complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHKUN_002265"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO6660516.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHKUN_002266"
                     /pseudo
     CDS             complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHKUN_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHKUN_002267"
     CDS             7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHKUN_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO6660517.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHKUN_002268"
     CDS             8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHKUN_002268"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO6660518.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHKUN_002269"
     CDS             complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHKUN_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO6660519.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHKUN_002270"
     CDS             complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHKUN_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO6660520.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHKUN_002271"
     CDS             complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHKUN_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO6660521.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHKUN_002272"
     CDS             13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHKUN_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO6660522.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHKUN_002273"
     CDS             complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHKUN_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO6660523.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            15792..17228
                     /gene="aspA"
                     /locus_tag="ACHKUN_002274"
     CDS             15792..17228
                     /gene="aspA"
                     /locus_tag="ACHKUN_002274"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO6660524.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHKUN_002275"
     CDS             17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHKUN_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO6660525.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18763..19101
                     /gene="cutA"
                     /locus_tag="ACHKUN_002276"
     CDS             18763..19101
                     /gene="cutA"
                     /locus_tag="ACHKUN_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO6660526.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHKUN_002277"
     CDS             19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHKUN_002277"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO6660527.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHKUN_002278"
     CDS             20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHKUN_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6660528.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21493..21568
                     /locus_tag="ACHKUN_002279"
     tRNA            21493..21568
                     /locus_tag="ACHKUN_002279"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21526..21528,aa:Phe,seq:gaa)
BASE COUNT         5046 a         5630 c         5578 g         5384 t
ORIGIN      
        1 caatatggaa accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca
       61 agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc
      121 gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt
      181 cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa
      241 agaagcgcag gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta
      301 tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa
      361 atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg
      421 cacaaatttg ccgtcgacca gtacgttaat caaatcaaca acctgcattt gcgcagcgtt
      481 gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg
      541 gatgcgtttt accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc
      601 ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa taatctggtc
      661 ttccattgct ttagtaaatg tctgaccgga atttacccgc catgtgcttt tgttatagca
      721 accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt
      781 gacgatgtcg acaggatagt actgatgata attcattgtt tcgcctggct tatcagtccg
      841 cgtataggat gcatccggta atcaacgcct gatgcgacgc tgtcgcgtct tatcagacct
      901 acaatcgttg ttgccatgta ggccggataa ggcgtttacg ccgcatccgg catttgctta
      961 acgtagagtg aagattaacc tatctgccca ttccccaaat gtttaacgcg gcgcttaact
     1021 tcttcctgct taccggcgtt aaatggacgt gcatccgggc tacctaaata tccgcacacg
     1081 cggcgagtca ccgacacacg ggaggcgtca tggttaccac atttcgggca agtgaagcct
     1141 ttgctggtgc actcgaactc accggtaaag ccacactcgt agcactcatc aatcggtgta
     1201 ttggtgccgt aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc
     1261 aggttgtgct gaatgtttgg atactcgccg tagcaaatga aaccaccgtt cgccagcggc
     1321 gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg
     1381 tggaaactgt tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagca
     1441 gtatcgagac ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa
     1501 ccataacccg tttcttcttt ccactgatcc actgcctggc gcagacgttc aacaatggcg
     1561 atacctttcg cgcgaagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta
     1621 atggtttcgt ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt
     1681 tcagaaacat catcgtcagc attcagacgt acgccacaag caccttccat atagaggatc
     1741 ggggccacgc gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt
     1801 gccagcacca gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc
     1861 agagcaatac gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc
     1921 tgctcgccgt tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta
     1981 aacgaaccgg tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg
     2041 cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc
     2101 agaccatcgc gaatcgcaaa caccagtttc gggaacaccg cagttttacg gtttttaccg
     2161 aggcctgcga tacggttacg caggatcgat tcctgaatca ggcgcgattc ccagctggtg
     2221 cccagaccaa aaccaaaggt tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt
     2281 acttcgtact ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagtttgcg
     2341 tagccttcgg cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg
     2401 gcggtaacaa acggcgccag cacttcatcg atacggttaa tggtggtgcc gccataaata
     2461 tggctggcga cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc
     2521 ggcggttcaa tctcggcgtt ccccatctta aagccctggg tcagcatgcc tttcaggtcg
     2581 atcaacatgc agttgaacat cgggaagaac ggcgagtaat cgagatcgtg atagtgaata
     2641 tcgccacgct catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatagtgt
     2701 ttagccacga tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg
     2761 gcgttttcgt tgagtaacga ggcgttggtc tgctcgacca gaccacggat ctcctggttc
     2821 aggcgaccgc gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga
     2881 gccagttgtt tgtatggacc cgacatcagc tggttttcaa ctgcggtctg gatctcgttg
     2941 atatccacct ggttgcggcc ctgcatctgc tcgctgacaa ccgcggcaac agtggcgcaa
     3001 taatcggcat catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc
     3061 tctgatttaa acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct
     3121 ccatattttt aagaacaggt tatccacaga aattgggaaa ggcattttcc ggttttttcg
     3181 tttcgttttc cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt
     3241 cgtggggagc tgtagtagca attatagtcg attaatacaa catattgggt tgggacgcat
     3301 tttaaagtct atatatagtg ctttgcatca aggatgtttt aacttttttt gatgtagctc
     3361 aaagtaaaaa gcagagcgta cggatgacgg gcgctacagc gttatgtaaa ttttttaatg
     3421 aatttgctgg ttgaaaaatc aacaaaaaca acatgctgac agacaaaacc ccggaatgac
     3481 cggcattacc ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc
     3541 cgtaaattca tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc
     3601 cagttgccgc gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg
     3661 accagcaagg tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc
     3721 agcagatcct ggtaattgcc ccatgtcagg atggcttctt gcttacgcag tgcgattaac
     3781 ttttggtagg tgtaaaacac cgaggagtca tcggccagcg cggcttctac gttgatttgt
     3841 tgatagttat cgcccaggcc aatccacggt tcgccagccg taaacccggc attatcgccg
     3901 ttgctccatt gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat
     3961 aactcgtcgg catcacgacc atcgttgcgc agctcggcaa acatattgag gctctccacg
     4021 tcgcgatagt cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccttgg
     4081 tagatatacg gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc
     4141 acgcggtatt caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac
     4201 cagaacaagg cattccatgc cacgttgtgc attccttgtt gccagtggcg gaacaatgtt
     4261 ttcaacgcca caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc
     4321 ttcaggtgat gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc
     4381 tggcaatgct caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta
     4441 aacacatcgc ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag
     4501 cgacgcccgt cgccatccag gtcttcaggg aaacgcgggt ctttggagat cagattcacc
     4561 acatccaggc gcaacccgtc gaccccacga tcggcccaga attcacagac ttttttcagc
     4621 tctgcgcgta ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc
     4681 aaatagtact gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc
     4741 cagttgttcg gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg
     4801 ctttctttgt tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc
     4861 atatcgagaa tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg
     4921 tccagcgtgc cgtagatggg atcaatcgcc gtatagttcg ctacgtcgta accgttatcg
     4981 acctggggag agatataaaa gggggttagc cagatggcat caacgcccag tttatgcaga
     5041 tagtccaggc gttggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga
     5101 aaactctttg gataaatctg gtagataacg ccgttttgcc accagtgggg aagattagtc
     5161 attacgttat tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat
     5221 atccagcgtg cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat
     5281 gatgatggcg ataaccattg ccagcgcaaa tacctgccag tagctcggtt gaatcgagag
     5341 aattcccggc aggccgccta cgccgatacc attcgccata acgccgttca ggccgcatag
     5401 taatcctgcc agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt
     5461 gatgccgtac attgcaggct cagtgacccc aagccaggcg gagatagcgg caggcacgga
     5521 gatctcgcgt tcattgtgct tgcggctgga aatgataatg cctatcacgg cggagccctg
     5581 agcgatattc gacagcgcaa tcagcggcca cactggcgtg ccacccatgc tttgaatcat
     5641 ctgcaaatca atagcaaggg tggtctggtg tacaccggtg atcaccagcg gggcgtacag
     5701 gaagccaaac aatgctgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc
     5761 aaaggcaacg ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa
     5821 caccgcgagg atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat
     5881 gcgtttaagg cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac
     5941 ctgcgcctga tagcctactt tggcgatgct gaacatgcca aagtcccaca cttccggcag
     6001 ctgctgcccg agcagataag cgttcatcag ctggggagaa accagtgtca cgccaagcac
     6061 gataccaagg atcggcgtgc cgcccatttt tttcaccgct gaccagcaaa taccgaccgg
     6121 caggtagaag aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga
     6181 agggtacatt tgcgccagcg tctgaccgtt gctcatgggc aaatcgccga tcacattgcg
     6241 aaaaccgagg atcaaaccgc cgctaatcaa cgcgggcagc agcgggaaga agatctccgc
     6301 gaaatgagag atcaactgct catgccattt catattctgc cgggcggctt tttttacctg
     6361 ctctttatca acctgcgcct gtccggttga cgcaatcagt gcttgatagt aatcacccac
     6421 attggtgcca atcaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg
     6481 gagttgctca atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg
     6541 agtaatacag tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg
     6601 atcgatatcc gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg
     6661 gttggctgca gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacacag
     6721 cgaagatcac aatttatcgt tcaggaaacg atcaggacag ggtggcgggg atgatgattt
     6781 gttgcggttc gctgcgcccg gttacctgtg cgatcaattg gcaggccgcc tggcgtccag
     6841 cttcggcgta accaggatct acggtgacga tctccggatg aaggaatttc attaacggcg
     6901 tattaccgac gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg
     6961 cgccaagtgc cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt
     7021 ttgctacgtt ctcatagcct tgcttcatag caagccctgg cagagcggca acgggatgca
     7081 gtttatgcgc tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt
     7141 cactgtgcgg cacgccgaga taactgatat tacgatgccc ctggtcatag agccgttgca
     7201 tcaggatctt gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac
     7261 gcgccagcag aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa
     7321 aaccgaacag cactacgccg tcgatattac gccgttttag gatccccaaa tgttcggcaa
     7381 ctaattgcgg ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaacg
     7441 ctggcagcat ggtttgaacg gcgagatttt ctgacaacga atccaggcgg gtaacaatga
     7501 tggcgaccac tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc
     7561 catgctgatt catcactgct tccacgcgct cgcgcgtgcg ctggctcacg ccgctttcgt
     7621 tattcagcac ccgggaaacc gtagatttcc ccacgccgct taagcgcgcg atgtctttga
     7681 tggtcagccg attttgcatc ctgttgtccc gtaacgtgtt gtttaattat ttgaggctaa
     7741 ctttacccgt gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca
     7801 ggtattatat cgccataaat gctacgaata ttattggatt ctccttatta tttgcggcgc
     7861 ttttttcact taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat
     7921 taaaactttt tcggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg
     7981 ttttaacgtc cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca
     8041 tttctgtact gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg
     8101 gctcattcgc catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca
     8161 gacagtgaat acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat
     8221 gcccgaagaa gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga
     8281 agtggaatct gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc
     8341 gtggtgggta catttatggg tctgctatcg caaccccttt aatattttac tcaccattct
     8401 cggcgctatt tcttacgcca cggaagattt atttgccgcg ggcgttatcg cgctaatggt
     8461 cgccatttct acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc
     8521 cctgaaagcg atggtcagca atactgcgac ggtgctgcgc gtaattaacg acaaaggcga
     8581 aaatggctgg ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc
     8641 ggcgggggat atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc
     8701 tcaggcgtcg ttaaccggtg agtctctgcc cgtagaaaaa gcagctacca ctcgccagcc
     8761 ggagcacagc aatccgctgg agtgcgacac cctgtgtttt atgggcacca ccgtggtgag
     8821 cggcacggca caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc
     8881 ggggcgtgtt agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt
     8941 cagtatgctg ctgattcgct ttatgctggt gatggctccg gtggtgctgt taatcaatgg
     9001 ttacaccaaa ggcgactggt gggaagcggc actgtttgcg ctttcggtcg cggtaggctt
     9061 aacgccggaa atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct
     9121 gtcgaaacag aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga
     9181 tattctgtgc actgataaaa ctggcaccct gacacaggac aaaattgtgc tggagaatca
     9241 taccgatatc tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca
     9301 ttatcagacc ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga
     9361 gtcagcgcgc tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga
     9421 gcgtcgccgg atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa
     9481 aggtgcattg caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt
     9541 gccgctcgat gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca
     9601 ggggctgcgc gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca
     9661 gcgggcggat gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa
     9721 agagacaact gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct
     9781 tactggcgac agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg
     9841 agaggtggtc attggtagtg atattgaaac gctatctgac gacgaactgg caaatctcgc
     9901 acagcgcacc acgctgtttg ctcgtctgac gccgatgcat aaagaacgca ttgtgacctt
     9961 actgaagcgc gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc
    10021 cttacgcgct gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc
    10081 ggctgatatc atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg
    10141 acgtcgcact ttcgccaaca tgctgaaata catcaaaatg acggcgagct ctaacttcgg
    10201 taatgtgttc agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt
    10261 acacttgctt attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa
    10321 cgtcgacgac gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt
    10381 tatgatcttc ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg
    10441 gtgggtattc catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt
    10501 ggtgggctta ctgtcgcaaa cgctgattgt gcatatgatc cgtacccgcc gtgtgccgtt
    10561 tattcagagc tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg
    10621 gatcgcattg ccgttttcac cgctggccag ttatctgcag ctgcaggcgc tgccgttaag
    10681 ctatttcccg tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa
    10741 agggttctat agccgtcgtt acggctggca gtaaagaata aactgggcac gatagccccg
    10801 attcagattg cagacaaagt gagcgttgtt catgccggat gcggcgtaaa tgccttatcc
    10861 ggcctacaaa ttcaatcaat tgcaggaatc acgtagatac tgataagcga agcgcatcag
    10921 gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact tataaacctg
    10981 accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg acgggctgct
    11041 gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat cgtttgctgc
    11101 gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc tttgcttacc
    11161 tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa tgcgtacact
    11221 ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta gcgcggcacc
    11281 gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg agctggacgc
    11341 cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga aaaaccccca
    11401 gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta catcatgccg
    11461 cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc atcatttttc
    11521 ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga agccgcgtac
    11581 tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat catgttgccg
    11641 tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac ggtgttagca
    11701 acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc ttccattgca
    11761 cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag gtcagccagt
    11821 ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc ttctaccgca
    11881 gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat ttcaacttcg
    11941 gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac gcgttcctgc
    12001 agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat ctgagcaaca
    12061 cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt gtctttgttg
    12121 atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag ctccataccg
    12181 atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag catagcttta
    12241 cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc acgcatggtg
    12301 ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat cagcagcggt
    12361 ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt agagattttc
    12421 ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc cggcttgttg
    12481 atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac gtccagttcg
    12541 tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac tttgtccatc
    12601 gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt accaacctga
    12661 gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc aactgcagcg
    12721 gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc agcaacagct
    12781 ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt accgtcgcct
    12841 gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat attttcgaac
    12901 ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat ggtcggtgca
    12961 ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac tttcactgca
    13021 tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc gaattttacg
    13081 tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt gcgaggatta
    13141 cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt cattgtcgat
    13201 cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa ctttcacatc
    13261 cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca gcacttcgcc
    13321 gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag atttagtttc
    13381 aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca ttgataactc
    13441 tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg tttcccggct
    13501 ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag aaaaaaaatt
    13561 ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc gcaatttctt
    13621 catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca gttgcccaaa
    13681 atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg gggctggccc
    13741 aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc gttcctgcgt
    13801 tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg attatcttag
    13861 tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc gcaggcggtg
    13921 tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc ggctggttgt
    13981 ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc gggttcggcc
    14041 aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt acgctggcgc
    14101 tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa accgttattg
    14161 ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc aaacctgcga
    14221 atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct gcgttatcag
    14281 tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg gaatttaaaa
    14341 atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca ggattagtct
    14401 actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa atggcggcgg
    14461 cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta tggattgcct
    14521 gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag agcttcgccc
    14581 gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc ctctcttctc
    14641 gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg agcacgttgg
    14701 tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat ggcatcttta
    14761 ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga cgttatcgac
    14821 tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc ggctggaaaa
    14881 gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa cgaaaaacgc
    14941 cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg atcgtcttta
    15001 tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa agtattaccg
    15061 ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa atgcggcatc
    15121 aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc gaggaagtcg
    15181 gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac acttttaatc
    15241 atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac gaaattctta
    15301 aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac cagcacgagg
    15361 gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc aatatagaca
    15421 taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa tagcagccgg
    15481 taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt ggtcattagc
    15541 aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg tgatctattt
    15601 cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat tacggtagaa
    15661 atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat cgtcggtcga
    15721 aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc agcttgaaaa
    15781 agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt accagggaag
    15841 ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac ttctatatca
    15901 gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg gttaaaaaag
    15961 ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg aatgccatca
    16021 ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc ccggtagacg
    16081 tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg ctggccaata
    16141 tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac ccgaacgacc
    16201 atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt atcgcagttt
    16261 actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc tttgaacgta
    16321 aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag gacgcagtac
    16381 cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa gaagtgaaaa
    16441 atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca gcaatcggta
    16501 ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg gctgaagtca
    16561 ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac tgcggcgctt
    16621 atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa atctgtaacg
    16681 acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac ctgccggaac
    16741 tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg gaagtggtta
    16801 accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca gcagaagcag
    16861 gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc gaatccgttc
    16921 acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc atcactgcta
    16981 acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact tacctgaacc
    17041 cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa accggtaaga
    17101 gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt gacgatattt
    17161 tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact gatgaaagcg
    17221 aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg tgcctttttt
    17281 cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa acaaggaagg
    17341 ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg ggcgccagat
    17401 tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt gccgctattg
    17461 gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg gttatcgccg
    17521 ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag acagaaaagc
    17581 tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc tatttcctga
    17641 ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc gctgaagttg
    17701 cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta tccgcgcaga
    17761 ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc gtgatggaag
    17821 gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc ctgctggcgg
    17881 ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct gacgatccga
    17941 tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag cagattgaaa
    18001 tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt ggcgtggtta
    18061 tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg atgaacacca
    18121 ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt atctgtaaag
    18181 tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc gcctgtattt
    18241 gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc gactggatca
    18301 aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc atcttcttct
    18361 ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg ccgatggccc
    18421 tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg tctggtctgt
    18481 tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac acgggtacaa
    18541 cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact ctgggtgttg
    18601 ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga ccaatcgcgg
    18661 ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc cgttgtatag
    18721 tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaagaaagt
    18781 tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac agcccaggat
    18841 ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct
    18901 acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta
    18961 aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat
    19021 caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg
    19081 ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt gccggattat
    19141 tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct tttgattttc
    19201 agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac tacctctacc
    19261 gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag
    19321 gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc
    19381 ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct
    19441 gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg
    19501 tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag cacaccgcgc
    19561 aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat
    19621 gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa cagcggctct
    19681 ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca ctgacttaca
    19741 cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc
    19801 catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca atgtttggct
    19861 tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg agcaatcgcc
    19921 aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt
    19981 caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca
    20041 tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc
    20101 taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca
    20161 aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg
    20221 gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt ggctgggcct
    20281 ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg
    20341 cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg
    20401 cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcagg
    20461 cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac tggtgcgtcg
    20521 cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag
    20581 acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg gcactgttaa
    20641 agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc
    20701 atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg
    20761 atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg
    20821 aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc
    20881 tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag
    20941 acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc
    21001 gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg gcacgttatc
    21061 aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt atcgctgcct
    21121 gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg
    21181 cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat ccggcgatgg
    21241 tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta
    21301 gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct
    21361 gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt tgccgcaatc
    21421 ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga ttacggttta
    21481 atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc
    21541 cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca cggaggtccg
    21601 tttttcgttt cagaacacca tgatttaagt gttcttcc
//
LOCUS       DAZFRJ010000066        21405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000066 DAZFRJ010000000
VERSION     DAZFRJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.066"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(422..1210)
                     /gene="phoH"
                     /locus_tag="ACHKUN_002280"
     CDS             complement(422..1210)
                     /gene="phoH"
                     /locus_tag="ACHKUN_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO6660529.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1829..3100)
                     /gene="efeB"
                     /locus_tag="ACHKUN_002281"
     CDS             complement(1829..3100)
                     /gene="efeB"
                     /locus_tag="ACHKUN_002281"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO6660530.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(3106..4233)
                     /gene="efeO"
                     /locus_tag="ACHKUN_002282"
     CDS             complement(3106..4233)
                     /gene="efeO"
                     /locus_tag="ACHKUN_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO6660531.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4291..5121)
                     /gene="efeU"
                     /locus_tag="ACHKUN_002283"
     CDS             complement(4291..5121)
                     /gene="efeU"
                     /locus_tag="ACHKUN_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO6660532.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5787..7295)
                     /gene="putP"
                     /locus_tag="ACHKUN_002284"
     CDS             complement(5787..7295)
                     /gene="putP"
                     /locus_tag="ACHKUN_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO6660533.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7454..7663)
                     /locus_tag="ACHKUN_002285"
     CDS             complement(7454..7663)
                     /locus_tag="ACHKUN_002285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660534.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7718..11680
                     /gene="putA"
                     /locus_tag="ACHKUN_002286"
     CDS             7718..11680
                     /gene="putA"
                     /locus_tag="ACHKUN_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6660535.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11720..12358)
                     /gene="rutR"
                     /locus_tag="ACHKUN_002287"
     CDS             complement(11720..12358)
                     /gene="rutR"
                     /locus_tag="ACHKUN_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO6660536.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            12646..13737
                     /gene="rutA"
                     /locus_tag="ACHKUN_002288"
     CDS             12646..13737
                     /gene="rutA"
                     /locus_tag="ACHKUN_002288"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO6660537.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            13737..14429
                     /gene="rutB"
                     /locus_tag="ACHKUN_002289"
     CDS             13737..14429
                     /gene="rutB"
                     /locus_tag="ACHKUN_002289"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO6660538.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            14441..14827
                     /gene="rutC"
                     /locus_tag="ACHKUN_002290"
     CDS             14441..14827
                     /gene="rutC"
                     /locus_tag="ACHKUN_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO6660539.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14835..15635
                     /gene="rutD"
                     /locus_tag="ACHKUN_002291"
     CDS             14835..15635
                     /gene="rutD"
                     /locus_tag="ACHKUN_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO6660540.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            15645..16235
                     /gene="rutE"
                     /locus_tag="ACHKUN_002292"
     CDS             15645..16235
                     /gene="rutE"
                     /locus_tag="ACHKUN_002292"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO6660541.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            16246..16740
                     /gene="rutF"
                     /locus_tag="ACHKUN_002293"
     CDS             16246..16740
                     /gene="rutF"
                     /locus_tag="ACHKUN_002293"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO6660542.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            16761..18089
                     /gene="rutG"
                     /locus_tag="ACHKUN_002294"
     CDS             16761..18089
                     /gene="rutG"
                     /locus_tag="ACHKUN_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO6660543.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(18172..18345)
                     /gene="ymdF"
                     /locus_tag="ACHKUN_002295"
     CDS             complement(18172..18345)
                     /gene="ymdF"
                     /locus_tag="ACHKUN_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6660544.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18718..19314
                     /gene="wrbA"
                     /locus_tag="ACHKUN_002296"
     CDS             18718..19314
                     /gene="wrbA"
                     /locus_tag="ACHKUN_002296"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6660545.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            19335..19562
                     /gene="yccJ"
                     /locus_tag="ACHKUN_002297"
     CDS             19335..19562
                     /gene="yccJ"
                     /locus_tag="ACHKUN_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO6660546.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(19600..20841)
                     /gene="agp"
                     /locus_tag="ACHKUN_002298"
     CDS             complement(19600..20841)
                     /gene="agp"
                     /locus_tag="ACHKUN_002298"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO6660547.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(21133..>21405)
                     /locus_tag="ACHKUN_002299"
     CDS             complement(21133..>21405)
                     /locus_tag="ACHKUN_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660548.1"
                     /translation="PEQKVNEVKNFLTALSDSICSLFIVNGNVASISLGNESFSTDDD
                     LEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQIDFLTRYAIY"
BASE COUNT         5064 a         5748 c         5526 g         5067 t
ORIGIN      
        1 aaaataacaa cacacaatat tagcaaagaa ttaattaaga ttaaccttac atttataata
       61 ttatccctaa agaataaatc agaggaatat attcggtgtt aaacagtcag tcaaagtata
      121 gcccattgtt gattaaacct cataagcaaa attggcacca gatctcctgt acaggttgaa
      181 tggccgaaaa aagataggca gatgctcatt tttttggctt ctggaagaaa aactctccgc
      241 gctggcagta ttacactggt ctgatgctat gacgaaaaat gcctggtaag agtatctaaa
      301 ccagactcca gttcagctta tcaacacgct caaagtcgcc cccctgaacg ctaacctgaa
      361 gtgaaaatct gttgaaataa aacaaagccc ggttcgcccg ggctctgcac cgataataca
      421 cttaactgta ggcatgcagc gtacgttggc aaagtgccga acgtacgcag tcctctttac
      481 cgaaccggac gatcccaacc atttcatctt cttcgaaacg ttccagcgcg tcacttaatc
      541 cggagcacac gccgcgaggc aaatcgcatt gcgtgatatc accgttgacg ataaccgtca
      601 cgttctcccc gaggcgggtt aaaaacattt tcatttgcgc ggcagtcaca ttctgcgcct
      661 cgtcaagaat gacgactgca ttttcaaagg tacgtccacg catataggcg aacggcgcaa
      721 tttccacctt ccctatttcc ggtcgcaggc agtactgcat aaaggaagcc cctaagcgcc
      781 ggaccagcac gtcgtagacc gggcgaaaat agggagcaaa cttttctgcg atatctccag
      841 gtaagaagcc aagatcttca tcagcttgca gaactggacg ggtgacgata atcctgtcga
      901 catccttatg tatcagggct tctgccgctt ttgctgcgct gatccaggtt tttccgcacc
      961 cggcttcgcc cgtggcgaat atcagctgct tactctcaat agccttcaga tagtgcaatt
     1021 gcgcttcatt tcgcgcgagg atgggcgaag tatcgcgact gtcgcgggcc ataccaatgg
     1081 cttctacgcc gcccatctgc acaagcgagg tgaccgattc ttcttcacgc tgcttatggc
     1141 tgcgcgaatc ccgtctcagc acacgttttg cctcgcgacg agctttgatc actgcttttt
     1201 gtcttcccat ggagagcacc ttgagttgtt tgtattcatc acacgcgccg ttggcagcgc
     1261 gattatgcgc acgaacatca gagggttggc ttccttgtaa gccatagttt gcttttggat
     1321 aaaatgccga aaaacggcta cgcacaccgt ttgcggcgtc ggtaacacga gtaaagagag
     1381 gaagtgagaa aaatacagag tgacgaagtt gctaccggag gagaaatctc cgcaagtggt
     1441 ggagcgtagc ttaacaagac attgtccaga acaggacgtt accatccgcg atctccatag
     1501 tgactgacta tcactgccgg gaacttccgc tgctacttaa taattacaac agatctcgca
     1561 ttcattgcaa caatatattc acttatattt aaccataaaa caccatttca gtgacattag
     1621 tttctactgg aaagatgaca gagtgatgac agatatgaaa aaagctgtgt gctttcagca
     1681 ggattcgcat caggcatctg cgcacgaacg ccggatgcgg cgtgaacgcc ttatccgcct
     1741 accgaacccg caccgattgt aggcataaga agacgcgcaa gcatcgcatc aggcatctgc
     1801 gcaagaacgc cttatccgtc tataaacatt aaacccgcaa taacgcgctt ccgagataat
     1861 cgttcgcgtc cttcactccc ggcagcgcaa aaaaataacc gccgccgata ggtttaacgt
     1921 attcctccag cgcttcgcca ttgagccttt tttgtactgt caggaagcct ttttccagat
     1981 cgtgttggta acagacaaac agtagcccca tatccagttg cccggagttg gtgacgccca
     2041 gtgaataact gtagccacga cgcagcatca ggctggactc actctccgcc gtgcggggat
     2101 tcgccagccg gatatggctg tccagcgcaa tcaccttccc ttccggatcg ctggcgtaat
     2161 cgggaacatc atgctcatgc tgcattccca gcggcgcacc ggtttgctta tcacgaccaa
     2221 aaatcgtctg ctgttctttc agcggagttc tgtcccaaaa ttccactcga aactgaatca
     2281 agcgtactgc ctgatagctg ccaccgattg tccacgctgg ctcctgctga tctgccgtta
     2341 cccacaccac tttttgcatc aacttatcat tctggctatc gggattggca gtgccgtctt
     2401 tgaaacccag caaattaatc ggcgtctctt tgcctttact acgcgccgcg tgatcggaaa
     2461 taaacccttc ccgcttccag cgcacgctga gcaaatccgg cgtatgtttg atgatatcgc
     2521 gcagtgcatg gatcaccgtg tcctgggtgt tggcgcaaat ttgtagcaac acatcaccat
     2581 gacataacgc cgcatccagc gagtcgttgg gaaaacgcgt catcttctgc aactttttcg
     2641 gcatctgtgg cgcaaggcca aagcgctcat caaacaatga gtgacccacc gataacgtga
     2701 tggtgagatt atcgggcgca atgtagccgc caagaatgcc ggaatcgagt ggtggcaggc
     2761 gcggatttgg cgtttctggt gccgctccgc cctgagtcag aaaagcaaaa cgctgagtca
     2821 acaagcgaaa caaccgttca agatcggctt tatcgctggc aagcacatca aacgccacca
     2881 gcatcattgc ggcctgttgt ggcgtcagga tccctgcctg atgctcacca taaaacggct
     2941 gtttctcatt gcgtgcatcc ggtgaaagcg tacccggcgc actttgcgtt ttttgtgcat
     3001 gagcgaccgg acaacttccc gccagcgcca gcgcaccaat gcctttcagt aaacgtcggc
     3061 gtgacggttc attcacgccg tttttatctt catactgcat aatgcttaat ccaatcccag
     3121 cacaccgcga agttgcgcca gatcttccgc cagcgcagta atcggtcctt tcagtgcatt
     3181 ccggtcggca tcggtcaatt tgtcgtaggt ttcaaaaccg tctttagtac ggtatttcgc
     3241 cagaatggta tcgacctttt taaagttggc atcgactttt gccaacagtt ccgggttggc
     3301 tttttgcagt tgtggacgca gcagatcgac aattttctgc gagccttcaa cgttagcctg
     3361 gaaatcccac agatcggtgt ggctgtagcg atcttcttca ccgctgattt tgctggctgc
     3421 cacttcctca atcagtccgg ctgcaccgcc gaccactttt gaaggtggga aagccagttc
     3481 actgatgcgt ttttgcaaat cgaccacatc ggtataaagc tggtcagcgt actgatccat
     3541 gcctttggtg gtgttgtcgc caaacaatgc tttttccaga cggtggaaac cggtgaattt
     3601 tggatcagcg gctttttgct cgtaatcatc ttcacgggcg tcaatgctgc catccagatc
     3661 ggagaacagt tcagcaatcg gttcaatgcg ctcatagtgc tggcgcgtcg gcgcatacag
     3721 tgctttcgct ttttcgatat cgcctgcttt aatcgcgtcg gtaaaggctt tggtgtcggt
     3781 caccagctgc gtggtttccg ccatgacata cgctttatat gcagtaattg caccaccaag
     3841 acttaacagc gcatcacttt gcgccgcatc cgccgttgcc tcacctttga cgatcaactt
     3901 ccctttcggg ttagtcagca gaccgcaggt catatcgtat tcgccaggct gtaaattcgc
     3961 cgtcattttc tggctaaaac caggggcgat attttcccgc tcttccacca ccatcacgcc
     4021 tttgaggatc tcccactcca gcgccttctg gctatggttc tgaataatga actgtgtttt
     4081 cccggcgtta accgtaatgg tcatcggttc gcactgctta tccgtcacgg tcactttgac
     4141 ctgcgtcaca tcagcggcgt tagccataaa agcagaagaa aacagcgcag ccacgctcaa
     4201 ctgcaatgcg ttacggcgga agttaatggt catgacgaga ctatcccttt aaagtatgtt
     4261 gtaactaagc aagagtttgc gtcgtatttg ttacgcggag cgagacgctg tcgcccctgc
     4321 gcgtggtggc agagcaaatg ccaccagcgc cgggatgaga taaataaacc agacggcgac
     4381 ttcgctgacg ctcggcgctt cctgatagcc aaaaatccct tccatcagcg tgccaaacag
     4441 cgagtgagtt gagagcaccg cactcatatc gaaggcgatt tcctgaaagt ggttccacaa
     4501 tccggcttca tgaaatgcgc gaatggcacc agctgccagc cctgcggcga cgaagagaat
     4561 aaacaggctg gtccatttaa aaaatgcacc aagattgagg cgaataccgc cccagtagag
     4621 caagaagcct agcaccacgg cagtagcaag accgagcatt gcacccagcg gcggccagat
     4681 cccgacatct tgttgaaatg ccgccagcag gaaaaagacc gactccagcc cttcccttgc
     4741 aacggcaaaa aagaccatca tcaccagcgc ccagccatga tgatttccac gctgcaatgc
     4801 gctatcgact gcctgctcca gttgtacttt gacgttgcgc gacactttgc gcatccagaa
     4861 aaccatccag gtaaggatca ccacggcgat caccgccacg ataccttcaa acagttcctg
     4921 ctctttttgc ggaaattcgc cggtggtttc gttaatgaag atccccaagc ccaggcacaa
     4981 cgcagcggca agcaacacgc caatccacat cacaccaatc catcggcctc gctgggtacg
     5041 cttaagatag ctggcaatca aactgacaat cagcgcggct tcaagtcctt cgcgcaacat
     5101 aatgagaaac ggaacaaaca tgccacccat ccttaaacgt ttttctgagt caatctatgc
     5161 aaagaaaagt aaatcgcagt gatagcgatt atcattacgg taaagaaaaa tacaagcgga
     5221 atgtttataa aatgatgttg aaatgtaaac aaagagaggt aatgccaggg gatataaacg
     5281 attattccag gtacaaagat gggattgttt acaacgagcc atgtgttctt gccggatgcc
     5341 gcgtaaacgc cgtatccagc cgacaaaacc gtcctgactc aatacatcaa aacttccatg
     5401 taaaactgat aagcgggtgt atcaagcaat ttcgtccatc ctcgattatc aaatctaact
     5461 ttttgcccgg aacataagaa cagcgcgggt gttggtggcg tcaaaatcgc tgaaacgttc
     5521 cccgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgtcttac aggacgttac
     5581 ctcgcgcccg acccggtagc cgaaggggat aagctgaagg taccacgcag cggcgatttt
     5641 gttcgccaga gcccgggggt gcagggggcc gcggcgattg gccgccccct gcgcgctccc
     5701 tgcaccattc aatttatatg gcgaggcaca ttaagggagc gcaacccgct gctgaagcca
     5761 aacaaaaccg ccgcaggcta agtcccttag ctttcctgca accgtgacgg cggagccgaa
     5821 tgatagtgcg catcggcctc ggcaaagcgt ttttgcatcg ccgctgacgg cgctttaccc
     5881 agcaaactaa acactacaat cccgatactg ccgaagatga agcccggaat aatttcgtag
     5941 agtcccagcc agccgaactg cttccagacg ataaccgtca gcgcaccgat gatcatcccc
     6001 gccagcgcac cgttgcgcgt catgcgtgac cacatcaccg agaacagcac caccggacca
     6061 aacgccgcgc caaagcctgc ccacgcgtag ctcactaagc ccagcacgcg gttttccggg
     6121 tttgccgcca gcgcaatcgc caccagcgcc accaccagca ccatcacacg tcctacccac
     6181 accagctctt tctggctggc ctgtttacgc agaaacgctt tgtataaatc ttcggtaatt
     6241 gcactggaac ataccagcag ctggcaactt aaggttgaca ttaccgccgc cagaatcgcc
     6301 gacagcagaa tcccggcaat ccacgggtta aacagaattt gcgccagttc gataaacacg
     6361 cgctcggcgt tctggtttac cgcgcctgct accgccgggt gctcattaaa gtaagcaatc
     6421 ccaaagaagc caacagccac cgcccctgcc aggcagagga tcatccaggt catactgata
     6481 cgacgcgcat ggacaatgct gtggtgagag tctgccgcca taaaacgcgc caggatgtgc
     6541 ggctgcccga agtaacccag cccccaaccc atcagtgaga taatggcgac aaagttcagc
     6601 cctttgagca tatcaacgtt ttcgatgctc ttttgtttga tcacttccag cgagtcacca
     6661 aagccaccga cactgataat gacgataacc ggcgtcagga tcagggcaaa aatcatcagg
     6721 ctggcctgta cagtgtcagt ccagctcacc gcgaggaaac cgccaataaa ggtgtaaagg
     6781 atcgtcgccg cagctcccgc ccacagagcc gtttcgtagc tcatgccaaa ggtactttca
     6841 aacagacgcg cgcctgccac aatgcccgaa gcgcaataaa tggtgaagaa cagcaaaatc
     6901 accagcgcgg agataatgcg caaaatgcgg cttttatctt caaagcgccc ggtgaaataa
     6961 tccggcaatg ttaaggcgtt attgttgtat tcggtatgca cacgcaaccg ccccgccacc
     7021 agcttccagt tgatccacgc gcctaatgtc aggccaatgg cgatccaact ttcggaaatc
     7081 ccggaaagaa aaaccgcgcc cggcaacccc attaacagcc agccgctcat atccgacgca
     7141 cccgccgata atgccgtcac gaatggccca aggctacgac cgcccagaat atagtcgtca
     7201 aagtttttcg ttgatcgcca ggcgataaac ccaatcaata tcatgccaaa gatatagaca
     7261 caaaatgtca ccaacatcgg tgtgctaata gccatctaaa gtctccaaaa aattattatc
     7321 ggcaatgtcg aaacttgccg ttatatctgc caccggaacg gggtaacaga gtttatgttt
     7381 taccagggcg accgtatcct gccggaagcg ctggttattc acaatcgatt taacacacca
     7441 tttacattaa attttagtgc tcagcgacac tatttttcat caggttgcac tctctcacat
     7501 tttttgcggt tgcacctttc aaaaatgtta actgccgcag agaaaaagtc tgagttattt
     7561 ttttaatccc tgtcatatcg atttctttta ttaacatttc attcattttt aagcttgcta
     7621 cgcatgtcac atttaacatg gttgcacaaa gttgcaacat catggatatt tcacgataac
     7681 gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca ccatgggggt
     7741 taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta tcgatcgcac
     7801 gccacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg aaaacagcga
     7861 tactctgccg gagctacctg cgctgctttc tggcgctgcc aatgagagcg atgaagcacc
     7921 gactccggca gaggaaccac accaaccatt cctcgacttt gccgagcaaa ttttgcccct
     7981 gtcggtttcc cgcgccgcga tcacagcggc ctatcgccgc ccggaaaccg aagcggtttc
     8041 tatgctgctg gaacaagccc gcctgccgca gccagttgct gaacaggcgc acaaactggc
     8101 gtatcaactg gccgataaac tgcgtaatca aaaaaacgcc agtggtcgcg caggtatggt
     8161 tcaggggtta ttgcaggagt tttcgttgtc atcgcaggaa ggcgtggcgc tgatgtgtct
     8221 ggcggaagcg ttgttgcgta ttcccgacaa agccacccgt gatgcgttaa ttcgcgacaa
     8281 aatcagcaac ggtaactggc aatcacacat tggtcgtagc ccgtctttgt ttgttaatgc
     8341 cgccacctgg gggctgctgt ttactggcaa actggtttcc acccataacg aagccagcct
     8401 ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa ccgctgatcc gcaaaggtgt
     8461 ggatatggcg atgcgcctga tgggcgagca gttcgtcact ggcgaaacca tcgcggaagc
     8521 gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg atatgcttgg
     8581 cgaagctgca ctgactgccg cagatgcaca agcgtatatg gtttcctatc agcaggcgat
     8641 tcacgccatt ggtaaagcgt ccaatggtcg tggcatctat gaaggtccgg gcatttcaat
     8701 caagctgtcg gcgctgcatc cgcgttacag ccgcgcccaa tatgaccggg taatggaaga
     8761 gctttaccca cgtctgaaat cactcaccct gctggcgcgt cagtacgata ttggtatcaa
     8821 cattgacgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg aaaaactctg
     8881 tttcgagcct gagctggcag gctggaacgg tatcggtttt gttattcagg cttatcaaaa
     8941 acgctgccca ctggtgattg attacctgat tgatctcgcc acccgcagcc gtcgccgtct
     9001 gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgtg cgcagatgga
     9061 cggccttgaa ggctatccgg tttatacccg caaggtgtat accgacgttt catatctcgc
     9121 ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg cgacgcacaa
     9181 cgcccatacg ctggcagcga tttatcaact ggcaggtcag aactactatc cgggtcagta
     9241 cgagttccag tgcctgcatg gtatgggcga gccactgtat gagcaggtca ccgggaaagt
     9301 tgccgacggc aaacttaacc gtccgtgtcg tatttatgct ccggtcggta cccatgaaac
     9361 gctgttggcg tatctggtgc gtcgcctgct ggaaaacggt gctaacacct cgtttgttaa
     9421 ccgtattgcc gacacctctt tgccactgga tgaactggtc gccgatccgg tcactgctgt
     9481 agaaaaactg gcgcaacagg aagggcaaac tggattaccg catccgaaaa ttcccctgcc
     9541 gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg ctaacgaaca
     9601 ccggctggcc tcgctctctt ctgccctgct caatagtgca ctgcaaaaat ggcaggcctt
     9661 gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta accctgcgga
     9721 accgaaagat attgtgggct ttgtgcgtga agccacgccg cgtgaggtag aacaggcgct
     9781 ggaaagtgcg gttaataacg cgccaatctg gtttgccact cctccggttg aacgcgcggc
     9841 gattttgcat cgcgctgccg tgctaatgga aagccagatg cagcaactga ttggtattct
     9901 ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgcg aagcggtcga
     9961 ttttctccac tactatgccg gacaggtgcg ggatgatttc gctaacgaaa cccaccgtcc
    10021 attagggcct gtggtgtgta tcagtccgtg gaacttcccg ctggctattt tcaccgggca
    10081 gattgctgcc gcactggcgg caggtaacag cgtgctggca aaaccggcag aacaaacgcc
    10141 gctgattgcc gcgcaaggga tcgccatttt gctggaagcg ggtgtaccgc caggcgtggt
    10201 gcagttgctg ccaggtcagg gggaaaccgt gggcgcgcaa ctgacgggtg atgatcgcgt
    10261 acgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc gcaatatcgc
    10321 cagccgcctg gacgctcagg gtcgccctat tccgctcatc gctgaaaccg gcggcatgaa
    10381 cgcgatgatt gtcgattctt cagcactgac cgaacaggtc gtcgtggatg tactggcctc
    10441 ggcgttcgac agtgcgggtc agcgttgttc ggcgctgcgc gtgctgtgcc tgcaagatga
    10501 gattgccgac cacacgttga aaatgctgcg cggcgcaatg gccgaatgcc ggatgggtaa
    10561 tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga aagccaatat
    10621 tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg cggtgcggga
    10681 aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga cgctgatcga
    10741 actggatgac tttgccgaat tgcaaaaaga ggtctttggt ccggtgctgc atgtggtgcg
    10801 ttacaaccgt aaccagctac cagagctgat cgagcagatt aacgcttccg gctatggtct
    10861 gacgcttggt gtccatacgc gtattgatga aaccatcgcc caggtcactg gctcggccca
    10921 tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggtgggtg tgcagccatt
    10981 cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct atctctaccg
    11041 tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg ctcgcgcgtc aggatgcaga
    11101 gtatccggtc gatgcacagt tgaaagccgc attgactcag ccgctaaatg cactgcggga
    11161 atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg agctggcgca
    11221 ggcaggaaca caacgattgc tgccggggcc gacgggtgaa cgcaacacct ggacgctgct
    11281 gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag gatgcgctga ctcagctcgc
    11341 cgccgtgctg gcggtgggca gccaggtact gtggccggat gacgcgctgc atcgtcagct
    11401 agtaaaggca ttgccatcgg cagtcagcga acgtattcaa ctggcgaaag cggaaaatat
    11461 aaccgctcaa ccctttgatg cggtgatctt ccacggtgat tcggatcagc ttcgcgcatt
    11521 gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg gtgcagggtt ttgcccgtgg
    11581 cgaaagcaat atccttctgg aacggctgta tatcgaacgt tcgctgagtg tgaataccgc
    11641 tgccgctggc ggtaacgcca gtttaatgac tataggttaa acaggtagcc ggaggatgtt
    11701 acaacctcct ccggcatctt taacgtggtc gaatcccctc aataataatc cgctgcacgt
    11761 tttcaaccgt ttgattgaaa aatatctcat cgcgcaacgt cgcgcctgtc accgcctcca
    11821 cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcatgaaa atcaaatgct
    11881 gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc gccgatttct
    11941 catcaattaa tgccttcaaa tcgcccgtca gttcatccat taacagcggc gcgcctgcca
    12001 gcatctccat acagaacagg cgcgaagcct gcggataatc gcgggagact tccagcttca
    12061 gacggatgta ctcttttatc gccgccagcg gagcgaaatc ttcacgaaac gcttttaacg
    12121 gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc tcttttgacg
    12181 gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcgatc tgctccagcc
    12241 ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga atcgcttttt
    12301 tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct tgcgtcatgc
    12361 gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc ctcgaccttc
    12421 tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc gtttagtcca
    12481 ctttttacca gatattttag acaacacaat gttaacacat taataacatt cactttttaa
    12541 aaactggcat ccgctttgca aacaagccat tacgcggctg aaaaggatat gcaggatgca
    12601 gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa aattggcgta
    12661 ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca gtacatgccg
    12721 acctttgaac tgaataaagc cattgtgcaa aaagcggagc actaccattt cgatttcgcc
    12781 ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga tcacaacctt
    12841 gagtcgttca ccttgatggc ggggctggcg gccgtcacct cgcgcattca gatttacgcc
    12901 actgctgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc aaccatcgac
    12961 tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa gcccgagtat
    13021 gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga ctatctcacc
    13081 gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga aaagcgattt taaaggcgat
    13141 tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga gtgtccccat gaaagtgatc
    13201 tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc agtatgccga tttcaacttc
    13261 tgtttcggca aaggcgtaaa tacgcccacg gctttcgccc cgaccgctgc gcggatgaaa
    13321 caggccgcag agcaaaccgg gcgcgacgtt ggttcttatg tgttgtttat ggtgattgcc
    13381 gatgaaaccg acgatgccgc tcgcgccaaa tgggaacact acaaagcggg cgcggatgaa
    13441 gaggcgttaa gctggctaac cgaacaaagt cagaaagata cccgctccgg tactgatacc
    13501 aacgtccgtc agatggccga tcccacttcg gcggtaaaca tcaatatggg gacgttagtc
    13561 ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg caagcgtgcc tggtgccgaa
    13621 ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg aaaccttcgg cgagcgcatt
    13681 caaccactga tgcagtgccg cgcccatcta cctgcgctga ctcaggaggt ggcatgatga
    13741 caaccttaac cgctcgaccg gaagccatta ccttcgatcc gcagcaaagt gcgctgatcg
    13801 tggtggatat gcaaaacgct tatgccacgc caggcggcta cttagatctc gccgggtttg
    13861 atgtctcaac cactcgcccg gtcattgcca acattcaaac cgccgtgacc gcagcgcgag
    13921 cggcagggat gctgatcatc tggtttcaaa atggctggga tgaacagtat gtcgaagctg
    13981 gcggccccgg ctcaccgaat tttcataaat cgaacgccct gaaaaccatg cgtaagcagc
    14041 cgcagctgca ggggaaattg ctggcgaaag gctcctggga ttatcaactg gtggatgaac
    14101 tggtgccgca gcctggcgat attgtgctgc cgaagccgcg ctacagcggt ttcttcaata
    14161 caccgctgga cagcattttg cgcagccgtg gaatacgcca tctggttttc accggtatcg
    14221 ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt ttttctggag tatttcggcg
    14281 tggtgctgga agacgcaaca caccaggcgg ggccggaatt tgcacagaaa gccgcgttgt
    14341 tcaatatcga aacctttttt ggctgggtca gcgacgtcga aacgttctgc gacgcgcttt
    14401 ctcccacatc ctttgctcgt atcgcttaag gagtttaacg atgccaaaat ccgtaattat
    14461 tcccgctggc agcagcgcac cgctggcccc cttcgttccc ggcacgctgg ctgatggcgt
    14521 ggtgtatgtc tccggtacgc tggcttttga tcaacataat aacgtgctgt ttgccgatga
    14581 cccaaaggcg caaacccgcc acgttctgga aattatccgc aaggtgatcg agacggcggg
    14641 tggcacgatg gcggatgtga ccttcaacag catctttatt accgactgga aaaattacgc
    14701 cgcgattaac gaaatctacg ccgagttttt tccgggtgat aaaccggcgc gattctgcat
    14761 tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc gccacaattg cgcatatcgc
    14821 caagtgaggc cgcgatgaaa ctttcactct cacctccccc ttatgctgat acgcccgtag
    14881 tggtgttgat ttcgggtctt ggcggtagcg gcagttactg gttaccgcaa ctggcggtgc
    14941 tggagcagga gtatcaggta gtctgttacg accagcgcgg caccggcaat aatcccgaca
    15001 cgctggcaga agattacagt atcgcccaga tggcagcgga actgcatcag gcgctggtag
    15061 ccgcagggat tgagcattac gcggtggtcg gccatgcgct cggtgcgctg gtgggaatgc
    15121 agctggcgct ggattatccc gcgtcggtaa ctgtgctgat cagcgttaac ggctggctac
    15181 gaataaacgc ccatacgcgc cgctgttttc aggttcgcga acgattactg tatagcggcg
    15241 gcgcgcaggc atgggtggaa gcgcagccgt tgttcctcta tcccgccgac tggatggcgg
    15301 cccgcgcacc tcgcctggag gcagaagacg cgctggcact ggcgcatttt cagggcaaaa
    15361 ataatttact gcgtcgactt aacgccctca aacgcgctga ctttagtcac catgcggatc
    15421 gcatccgctg cccggtgcaa atcatctgcg ccagtgatga tctgctggtg ccatcagcat
    15481 gttccagtga gcttcatgcc gccctgcccg atagccagaa aatggtgatg ccctatggcg
    15541 gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc tctgttactc aacgggcttg
    15601 ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat tgctatgaac gaagccgtta
    15661 gcccaggtgc gcttagcacc ttgttcaccg atgcccgcac tcacaacggc tggcgggaga
    15721 cacccgtcag cgatgagacg ttacgggaga tttatgccct gatgaaatgg gggccgacat
    15781 cagctaactg ttctccggca cggatcgtgt ttatccgcac ggcagaagga aaagaacgtc
    15841 tgcgcccggc actttccagc ggcaatctgc aaaaaaccct gaccgcgccc gtcaccgcta
    15901 tcgtcgcctg ggacagtgaa ttttatgaac ggttaccgca actgtttcct cacggcgatg
    15961 cccgcagttg gtttacctcc agcccacaac ttgccgaaga aacagcgttt cgcaacagtt
    16021 ccatgcaggc ggcctatctg atcgtcgcct gccgtgcgct gggactggat accggcccga
    16081 tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt tgcgggcagc acgctgaaga
    16141 gcaatctgct gattaatatc ggctatggcg atagcagcaa actttttgcg cgcctgccac
    16201 gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc aaaccatgaa cattgtcgat
    16261 caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg cagtcaatat catcaccacg
    16321 gatggtccag ccgggcgcgc cgggttcacc gccagcgcgg tctgcagcgt gaccgacacg
    16381 ccgcctacat tactggtgtg cctgaatcgt ggggcgtccg tctggccggt attcaatgaa
    16441 aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg agccgctttc aaatcttttt
    16501 ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc gctggcagac gggtgtgacc
    16561 ggatgcccgc aactggaaga ggctctggtt tcgtttgact gccgtatcag ccaggtggtg
    16621 agcgtcggca cccacgacat tctgttttgc gccatcgaag cgattcatcg tcacgccaca
    16681 ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc tgatgcgccc tgcttgttaa
    16741 cctcctaaag gagacagctg atggcaatgt tcggttttcc tcactggcag ttaaaatcga
    16801 cctctacaga gagcggcgtg gtcgcgccgt atgaacgatt gccgtttgct cagacggcag
    16861 taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac ggtgttaatg ccgattctga
    16921 tggggctgga tcccaatctt tccattttaa tgtcggggat cggcacgtta ctgtttttct
    16981 ttattaccgg cgggcgcgtc cccagttatc tcggttccag cgcggctttt gtcggcgtgg
    17041 tgattgccgc gacgggcttt aacggtcagg gcattaaccc gaatatcagc attgcgctgg
    17101 gcgggattat cgcctgtggg ctggtttata ccgtcattgg cctggtggtg atgaaaattg
    17161 gtacacgctg gattgaacga ctaatgccgc cggtggtgac gggtgcggtg gtgatggcga
    17221 ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc ggcttcggcc tttgatagct
    17281 ggatggcggt aatgacggtg ctgtgtattg ggctggtggc ggtgtttacc cgtggcatga
    17341 tccagcggtt attgattctc gtaggtttaa ttgtcgcctg tctgctgtat ggcgtgatga
    17401 ccaacctttt agggctggga aaagccgtcg attttactct cgtcagccat gctgcctggt
    17461 tcggtttgcc acatttttcc actcctgctt ttaatagtca ggcaatgatg ctgattgcgc
    17521 ccgtggcggt gattctggtg gcggaaaact taggtcatct caaggcggtc gccgggatga
    17581 ccggacgcaa tatggacccg tatatggggc gggcattcgt cggtgatggg ctggcgacga
    17641 tgctttctgg ttctgtcggt ggcagcggcg taaccaccta cgccgagaat atcggtgtaa
    17701 tggccgtgac aaaagtctac tcgacgctgg tgtttgtcgc tgctgccgtc atcgccatgc
    17761 tgttgggctt ttcaccgaaa tttggcgcgc tgattcatac cattccggcc gcggtgattg
    17821 gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc aggcgcaagg atatgggtac
    17881 aaaaccgtgt cgatttaagc cagaacggta atttgattat ggtcgcagta accttagtgc
    17941 tgggcgcggg tgattttgct ctcacgctgg gcggttttac gttgggaggg attggtacag
    18001 caacctttgg cgcgatttta ctcaatgcgt tgttaagcag aaagttggtt gacgttccac
    18061 cgccagaagt tgttcatcag gagccataaa cagaaaaccg ccgggattac cggcggtgat
    18121 gactttttga cgagcctaag aaaaaaatgc attcgtcatt gattagcccg gctagttgtc
    18181 gcttttgccg tgactgctct tgccaccttt tttacctgct tcagatgcgc gctgcgggtc
    18241 attcttgaaa ttacccccgc tgtgctgtcc acctttttta cctgcttctg atgctctttc
    18301 gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt gccattactg acctccgtct
    18361 gggtatagac atcatattgc atagaattaa cgcggcatat ggcggctgcc atattttcgc
    18421 tacgtgggac taagcttagt gaactgacgc gggcagaccc gcgtttggta atatcctgca
    18481 acagcttctt agcgagaata gatatcatct caaataatta tccataagcc gctcttatga
    18541 ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga caaaattacg tgcttgtgaa
    18601 agttgttata aatcaaataa gtggttgtga aatttgcact ctgaaaagga cgtcttatct
    18661 ttaaataagt ggtagcgaat cgctacggaa tagagataac acgaggagtg gttagaaatg
    18721 gctaaagttc tggtgcttta ttattccatg tacggacata ttgaaacgat ggcacgcgca
    18781 gtcgctgagg gtgcaagcaa agtcgatggc gcagaagttg tcgttaagcg tgtaccggaa
    18841 accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa cgcaaactgc accggttgca
    18901 accccgcaag aactggccga ttacgacgcc attatttttg gtacacctac ccgctttggc
    18961 aacatgtccg gtcaaatgcg taccttcctc gaccagacgg gcggcctgtg ggcttccggc
    19021 gcactatacg gaaaactggc gagcgtcttt agttccaccg gtactggcgg cggtcaggaa
    19081 caaactatta cttcaacctg gacgaccctt gcgcatcacg gcatggtaat tgtccccatt
    19141 ggctacgcag cgcaggaatt atttgacgtt tcacaggttc gcggcggtac gccgtacggc
    19201 gcaaccacca tcgcaggcgg tgacggctca cgccagccaa gccaggaaga actgtctatt
    19261 gctcgttatc aaggggaata tgtcgcaggt ctggcagtta aacttaacgg ctaatcttca
    19321 acaggaggat acgcatgcca actcaagaag cgaaagctca tcacgtcggt gaatgggcat
    19381 ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt cgaagtcgcc gggtacgacg
    19441 aaaaaatggc ggaaaagatt tgggaagaag gtagcgatga agtgttagtc aaagcctttg
    19501 ctaagaccga taaagattcg cttttttggg gcgaacagac catcgaacgt aaaaacgttt
    19561 aaccagcgac tcccccactt ctcgcggggg agttttctgt tatttcaccg cctcattcaa
    19621 cacgctatca aacttatcca ttgggcagaa accgttggca tctatcgggc aaccgcttaa
    19681 ttccagcgtc acacgctgag caggtgcctg cagggttaac gcatcggcat tacgtaactg
    19741 ttccgcactc tgatacacat attcaatttt catcaaatcg cgattggctt tgctgtcatg
    19801 ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt tcgttctggt catgcaactg
    19861 atacggtttg aaatccagcg ccgttaacag agaggcaatg ttggagtcgt gcccaaccaa
    19921 cactgtaatt ttcggtgcgc tggtgcgatc ggtgaccaga gctttgtcga tataactgac
    19981 cagcggtttc gcaacattgc gcgccacttc cggtgaggta aacaggctgt cctggtagcc
    20041 gtttttaagc ttcgacaaca ccttccactg ctggtcagat ttgatttctc cccaggccac
    20101 ctgatccatc ggaaaacctt cgtaatattg caaagtaaac gcatctacca gcgagttgcc
    20161 gactttcagc ggcccggaaa cacctggttc ttgttgatac ttggcgctaa aggtattttt
    20221 gccatccacc agcgaacact gttgtttctc tttacaggca ggggaatctt tatagttaac
    20281 gattttttcc agtagttggt agctgtcggt aagctggagt ttgctgagct ctttctccat
    20341 tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg gtgatcaccg ggttaaaggt
    20401 tgggtccata gtgcccattt tttcctggtg atgcacagga atatcacacc ccgggaatgc
    20461 gccggtaata aagaactgtg cggtcgcaac ggtacgttgc agactattgg cataggcata
    20521 aacggtgtcc ggcggcgggc attcccccga tttcaccatc ccctgctgtg ccagccattc
    20581 acgcatgtaa tggcccatat acacttcgag cacgccacct ttggtggtga gttgcccacc
    20641 ggggacgtcc cattcgggcc atttattcgg cgtagactgc tccagcacac tgccattgtt
    20701 cgccagcggc gcacgtaagt tatggcggct catcatgagc acttgctgta gctgatagcc
    20761 ttccggtacg gtttgtgcct gagcatttga agcgagtaaa actatccctg ccacagttgc
    20821 ggcgattagc gttttgttca ttcctggcac ctcttttgtt atcaatcact aaagagtgtg
    20881 acagaaatat gacatttttc cgggaacagg ttcgcaaaat ggcttgttga atgcctgttt
    20941 ttctatatac atctttaaaa acaggcaagg tttgacgcaa actatgcctg attagctccc
    21001 tgcctcgcgt acgccgctga tgattacacc tgtggctaaa cagaatatca gcggcgttac
    21061 taatacagag gcaatagtac caataataag agaagtgagg ctaataacaa tggtttttag
    21121 cacatgcctt tgtcagtaaa ttgcataacg agttaaaaaa tcaatttgcg cattcctttc
    21181 cttattgttt aactcgtaag cctgagcaaa aagttcagga gggaggtaaa cgtcataatg
    21241 attgccagtg tttattaaat acccatactc aaggtcgtca tctgttgaaa aggattcatt
    21301 ccctaaggag attgatgcca cattaccatt aacaataaat aatgagcaaa tgctatctga
    21361 aagagcagtg aggaaatttt tcacctcatt aaccttctgc tctgg
//
LOCUS       DAZFRJ010000067        20841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000067 DAZFRJ010000000
VERSION     DAZFRJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.067"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(163..1812)
                     /gene="actP"
                     /locus_tag="ACHKUN_002300"
     CDS             complement(163..1812)
                     /gene="actP"
                     /locus_tag="ACHKUN_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO6660549.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1809..2123)
                     /gene="yjcH"
                     /locus_tag="ACHKUN_002301"
     CDS             complement(1809..2123)
                     /gene="yjcH"
                     /locus_tag="ACHKUN_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO6660550.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2324..4282)
                     /gene="acs"
                     /locus_tag="ACHKUN_002302"
     CDS             complement(2324..4282)
                     /gene="acs"
                     /locus_tag="ACHKUN_002302"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO6660551.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4674..6110
                     /gene="nrfA"
                     /locus_tag="ACHKUN_002303"
     CDS             4674..6110
                     /gene="nrfA"
                     /locus_tag="ACHKUN_002303"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO6660552.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6155..6721
                     /gene="nrfB"
                     /locus_tag="ACHKUN_002304"
     CDS             6155..6721
                     /gene="nrfB"
                     /locus_tag="ACHKUN_002304"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO6660553.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6718..7389
                     /gene="nrfC"
                     /locus_tag="ACHKUN_002305"
     CDS             6718..7389
                     /gene="nrfC"
                     /locus_tag="ACHKUN_002305"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO6660554.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7386..8342
                     /gene="nrfD"
                     /locus_tag="ACHKUN_002306"
     CDS             7386..8342
                     /gene="nrfD"
                     /locus_tag="ACHKUN_002306"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO6660555.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8362..10080
                     /gene="nrfE"
                     /locus_tag="ACHKUN_002307"
     CDS             8362..10080
                     /gene="nrfE"
                     /locus_tag="ACHKUN_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO6660556.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10073..10456
                     /gene="nrfF"
                     /locus_tag="ACHKUN_002308"
     CDS             10073..10456
                     /gene="nrfF"
                     /locus_tag="ACHKUN_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO6660557.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10453..11049
                     /gene="nrfG"
                     /locus_tag="ACHKUN_002309"
     CDS             10453..11049
                     /gene="nrfG"
                     /locus_tag="ACHKUN_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO6660558.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11391..12704
                     /gene="gltP"
                     /locus_tag="ACHKUN_002310"
     CDS             11391..12704
                     /gene="gltP"
                     /locus_tag="ACHKUN_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO6660559.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12782..13471)
                     /gene="yjcO"
                     /locus_tag="ACHKUN_002311"
     CDS             complement(12782..13471)
                     /gene="yjcO"
                     /locus_tag="ACHKUN_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO6660560.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13565..15712)
                     /gene="fdhF"
                     /locus_tag="ACHKUN_002312"
     CDS             complement(13565..15712)
                     /gene="fdhF"
                     /locus_tag="ACHKUN_002312"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15293..15295),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO6660561.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15910..17376)
                     /gene="mdtP"
                     /locus_tag="ACHKUN_002313"
     CDS             complement(15910..17376)
                     /gene="mdtP"
                     /locus_tag="ACHKUN_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO6660562.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNATLAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17373..19424)
                     /gene="mdtO"
                     /locus_tag="ACHKUN_002314"
     CDS             complement(17373..19424)
                     /gene="mdtO"
                     /locus_tag="ACHKUN_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO6660563.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19424..20455)
                     /gene="mdtN"
                     /locus_tag="ACHKUN_002315"
     CDS             complement(19424..20455)
                     /gene="mdtN"
                     /locus_tag="ACHKUN_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO6660564.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20474..20749)
                     /gene="ytcA"
                     /locus_tag="ACHKUN_002316"
     CDS             complement(20474..20749)
                     /gene="ytcA"
                     /locus_tag="ACHKUN_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO6660565.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4704 a         5519 c         5706 g         4912 t
ORIGIN      
        1 ttaacaagaa agaaaaggca tattctctgc attatcattt tctacgactg tcaaaaatcg
       61 ctcatttttt aatgagttta tttgtttaat attatgggaa aaggtgatgc atgctggaga
      121 ggaagagtat tccccggtca ggcgaccggg gaaggggaga gattaatgtg cgcggccttg
      181 ctcaacgcca aagccggtct gggagcggat aaactgtgca cggaacagtt cacgctcgcg
      241 cgcaccttcc gctgagttat cggttgccga gaagaaccag atgccgagga atgccacgct
      301 gatagagaac agcgccgggt attcatacgg gaagatggct ttttcgtgac caaggatctg
      361 tacccaaatc gtcgggccga ggatcatcag taccactgcg gtaatcagcc ccagccagcc
      421 acccatcatc gcgccacgag tggtcagttt cgaccagtac atagaaagca gaatgatcgg
      481 gaagttacag ctcgctgcga tggcaaacgc caggcctacc atgaaggcga tgttctgatt
      541 ctcaaacagc acgccgagga taatggcaat cacacctaaa atcagcacgg tgattttcga
      601 tacccgcagt tcttcacgtt cggtcgcgcc ttttttgaag acgttagcgt acaggtcatg
      661 cgaaaccgct gatgcgcccg ccagcgtcag accagcaacc accgcaagaa tggtggcaaa
      721 agcaaccgct gaaataaaac cgaggaacag gttgccgccc actgcattcg ccaggtgaac
      781 ggccgccatg ttgttaccac caatcagatg gcccgccgcg tctttatatt ccggattcgc
      841 accaaccagc atgatcgcgc cgaagccgat aataaaggtc agaatataga agtagcccat
      901 aaacccggtg gcgtagaaca cgctcttacg tgcttcgcgg gcatcgctga ctgtaaagaa
      961 gcgcatcaga atgtgcggca agcccgccgt accaaacatc agtcccagac ccagagagag
     1021 cgcggagatc ggatctttca ccagcccgcc cggcttcatg atgtcgacac ctttcgggtg
     1081 taccgccatc gcttcactga acagattgtt gaagctaaag ccgacgtgtt tcatcaccat
     1141 aaaggccata aagctggcac cgaacagcaa cagcacggct ttgataatct gtacccaagt
     1201 ggtcgccagc atgccgccga acaggacgta catcatcatc agcacgccga ccagcaccac
     1261 cgcaatgtga tagttaaggc caaacagcag ctcgatcagt ttacctgcgc ccaccatctg
     1321 ggcgataagg taaagcgcca ccaccaccag agaaccacag gccgaaagaa tacgaatcgg
     1381 cccttgtttc agacggtaag aggccacatc ggcaaaggtg tagcgcccca ggttacgcag
     1441 acgttcggca atcaggaaca aaatgatcgg ccagcccacc aggaagccca gcgagtaaat
     1501 taagccgtca tagccggagg taaacaccag cgcggagatc cccaagaatg aggcggcgga
     1561 catatagtcc ccggcaatcg ccagcccgtt ctggaagcca gtgatattgc cgcctgcggt
     1621 gtagtagtcg ctacgagaac gtacgcgttt tgatgcccag taggtaatgc cgagcgtaaa
     1681 cacgacgaaa atcaggaaca taataatcgc ctgccagttc gttggctggc gctctacggc
     1741 cccgctaata gcatccgcgg cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag
     1801 aactcttttc atgatgcttg tacctcatgc aggacttcat tattaagacg gtcgaattcg
     1861 ccgttcgccc gccagatgta gataccggtg agaacaaagg agatcacaat cactccaaca
     1921 ccaattggaa taccgcgtgt gacgctggtg ttcggattca gcggcgtgcc cagccagccg
     1981 ggcgcgaagg cgatcagtaa aataaagccg atataaactg ccagcataat aatcgacagg
     2041 atggtggcaa accgttgccg tttttcgact aactccctga aatgcgcatt gtcttctatc
     2101 cgctgataaa tcgtaccatt catcacagat tctccagagg taatgtaggg attgttttaa
     2161 ttcccgctcc cttatgggag aaggttaacg ctcgggtaac ccttgccgaa tgtaggccgg
     2221 ataaggcgtt atacgccgca tccggcaatc aatgcctgat gcgacgctgt cgcgtcttat
     2281 caggcctaca aaccgttacc gactcgcatc cggcaattgt gggttacgat ggcatcgcga
     2341 tagcctgctt ctcttcaagc agcttctcga ctacgccagg atcggcaagc gtcgaggtat
     2401 cgcccaggtt gctggtatcg cccgccgcaa ttttgcgcag aatacggcgc ataattttgc
     2461 cggagcgggt tttaggcagg gagtcggtcc agtgcagcac gtctggcgtc gccagcgggc
     2521 caatctcttt acgcacccag ttgcggactt ctgcgtacag ttctggtgac ggttcctccc
     2581 cgtgattaag cgtgacgtag gcgtagatcg cctgaccttt aatattgtgc ggaataccta
     2641 ctacggcggc ttcggcaatc ttcggatgcg ccaccagcgc cgactcaatc tctgccgtcc
     2701 ccagacggtg accggagacg ttcagcacgt cgtccacacg cccggtgatc cagtaatagc
     2761 catcttcatc gcgacgcgcg ccgtcgccgc tgaaatacat atttttgaag gtggagaagt
     2821 aggtctgttc aaaacgttcg tgatcgccaa acagcgtacg cgcctgaccc ggccaggagt
     2881 cggtgatcac caggctgcct tcggtagcgc cttccagcgg gttaccttcg ttatcgacca
     2941 gcgccggttg cacgccgaag aacggacgtg ttgccgaacc ggctttcagc tcggtagcgc
     3001 caggcagcgg cgtgatcatg aaaccgccag tttcggtctg ccaccaggta tcgaccaccg
     3061 gacatttctc gttgccgatt tttttccagt accactccca cgcttccggg ttaattggct
     3121 cgcccacgga accgagaatg cgcagcgacg aacggtcggt accctcgatc gctttatcgc
     3181 cttccgccat cagcgcgcgg atcgccgtgg gtgcggtata gagaatattg acctgatgct
     3241 tgtccaccac ctgcgccata cgggcaggcg tcggccagtt gggtacgcct tcaaacatca
     3301 gcgtggtcgc accgcaggcc agcgggccgt acagcaagta actgtgtccg gtcacccagc
     3361 ccacatcggc ggtgcaccag tagatatcac ccggatgata atcaaagaca tatttaaagg
     3421 tcagcgccgc gtacaccaga taaccgccgg tagtatgcag cacacctttt ggcttaccgg
     3481 tagaaccgga ggtgtagaga ataaacagcg gatcttcggc gttcatctct tccgcctggt
     3541 gctgatcgct cgcttgctca accaggtcgt gccaccacag gtcgcgccct tcctgccagt
     3601 caattttccc gccagtacgc ttcagtacca ccacatgctc tacgctggtg acgttcgggt
     3661 ttttcagcgc gtcatcaacg tttttcttca gcggaatact gcgcccggca cgcacacctt
     3721 cgtcggaagt gatcaccagt cgtgagttgg aatcaataat gcgcccggca acggcttccg
     3781 gcgagaagcc gccgaaaatc accgaatgca ccgcgccaat gcgggcgcag gccagcatcg
     3841 caaccgcggc ttccggcacc atcggcatat aaatcgccac cacatcacct tttttaatgc
     3901 ccagctcgag cagggtattg gcgaagcggc acacgtcgcg gtgcagctct ttatagctga
     3961 tatgtttgct ctggctggcg tcgtcgcctt cccagatgat ggcggtgcga tcgccgtttt
     4021 cttgcagatg gcggtcaagg cagtttgccg ccagattcag cgtgccgtcc tcgtaccatt
     4081 taatggacac attaccgggg gcaaaggagg tatttttcac cttctggtac ggtttgatcc
     4141 agtcgagaat ttttccctgt tcgccccaga aggtatcagg tacgttaata gattgttgat
     4201 acatcgcctc gtactgctga gggtttatca ggcaacggtc tgcgatgttg gcaggaatgg
     4261 tgtgtttgtg aatttggctc atgcttttgt tctccttgta ggatgttaat aatatgtggc
     4321 ataagcgtta aatgtagggg tattggcagt tttgtttagt atttgggcga cagatcacgc
     4381 aaaagtagaa ttgtgcaaat aaacggcagg gtaatttttg aaggtcacca acaaaagttg
     4441 attaattctt tgaggaacat gcagttatgc atgctgttga aaaagaggaa gatactgact
     4501 aactctaaag tggtatttta catgcactta caattgatta aagacaacat tcacagtgtg
     4561 gttatatgtt acacataggg gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt
     4621 gaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4681 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4741 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4801 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4861 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4921 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4981 aaaccctgcg taccggtgcg ccgaaaaacg ctgaagatgg tccgctaccg atggcgtgct
     5041 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     5101 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     5161 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5221 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5281 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5341 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5401 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5461 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5521 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5581 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5641 ctgctttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5701 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5761 cggaagccga aatgaagccg attcaggacg atatccgtca tgcccagtgg cgttgggatc
     5821 tggcgatcgc ttcccacggt attcatatgc acgcaccgga agaaggtcta cggatgctcg
     5881 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5941 aaggcatcac ccatgaaatc cagatcccgg atatctcgac caaagagaaa gcccagcagg
     6001 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     6061 tcccgcagtg ggaagaacag gcacgtaaaa atggtctgtt aagccaataa ccccgttccg
     6121 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6181 tgccggggtg ctggcgtcgg gcttgttgtg gagcctgaac ggaattaccg ctacccctgc
     6241 ggcgcaggca tctgatgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6301 tctggactgt cataaaccag ataccgaagg tatgcatgga aaacatgctt ccgttatcaa
     6361 cccgaataac aaactgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6421 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg ttggagagca
     6481 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6541 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6601 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6661 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6721 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6781 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6841 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcggga agtgaacaag
     6901 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6961 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     7021 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     7081 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     7141 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7201 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7261 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7321 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7381 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7441 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7501 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7561 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7621 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7681 tgctgtttca gctctacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7741 atttgcttgc cctgcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7801 gcctgctgac gcccgttcat cgcggactgg aaacattgat gctggtgttg gcggtgttgt
     7861 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7921 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7981 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcag tttgtacacc
     8041 gtatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcattt tttgtcggtc
     8101 tggcgctggg cgatgacggt aaagtgcgtg cgctggtggc ggcattaggt ggcggtttct
     8161 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8221 aaccgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8281 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8341 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8401 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8461 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtt cgcgctcctg
     8521 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8581 gtcgcgcaac atagctatag cctgctgtcg tgggaactca agctggcagc ggtgtggggc
     8641 ggccatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8701 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgttagc cgttttatct
     8761 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8821 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8881 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8941 ctggcgagtt tactgcgcgg cgagtttgat ggtgcctgcg ccagaatttg ttggcgctgg
     9001 gcactacctg gctggagcgc attaacggcg gggatcatcc tcggttcctg gtgggcctat
     9061 tgcgaactgg gctggggcgg ctggtggttc tgggatccgg tggaaaacgc ctctttatta
     9121 ccctggcttt ctgccactgc gctgctgcac agtttgtccc tgacacgcca gcgggagatt
     9181 ttccgccact ggtcgctgtt gctggcgata gtaactctga tgctgtcgct actgggtacc
     9241 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcactgga taacgtccgc
     9301 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9361 tggcgagcgc gggacggtgg tgcggtggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9421 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9481 tatccgatga tttacggcct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9541 aaccgcgcga cgttaccgtt tggcctgttg atgctggtgg tgattgtgct ggcgacgttt
     9601 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9661 gccgcgggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9721 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9781 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9841 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9901 tcaattcgct ggaacggcat ccatgactgg tatgcggtca tgggtgaaaa aaccggagcg
     9961 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
    10021 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
    10081 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
    10141 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10201 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10261 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10321 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10381 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10441 gagggcaaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10501 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10561 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10621 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10681 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10741 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct ggcgacggtg
    10801 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10861 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10921 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10981 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    11041 tcagattaat catctggtga tgaagattat gttattgagt tgtataaaaa ataatcgaat
    11101 gaacatatgc caaaaataat caccaatcag tattattgca gattaaacaa ataaaaatct
    11161 ttccataaca aatggttatt cattaatcct gccataaact cgcgttatta tgcattaatg
    11221 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11281 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgccctggca gtatgtcacg
    11341 ttctcgcgtt tctgaacggg gaacggcgct ccattgagga agttattctg atgaaaagta
    11401 taaaattcag cctggcctgg cagattctgt ttgctatggt gctgggcatt ctcctgggaa
    11461 gctacctgca ctaccatagc gacagccgcg actggctggt cgtcaatttg ctctctccgg
    11521 cgggtgatat cttcatccat ctgatcaaaa tgattgttgt gccgattgtg atctccacgc
    11581 tggtggtggg tatcgcgggt gttggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa
    11641 ccattattta cttcgaggtg atcaccaccg tcgccatcat tttggggatc actctggcga
    11701 acgtcttcca gcccggtgcc ggggtggata tgtcgcagct ggcgaccgtc gatatctcga
    11761 aatatcagag cactacggaa gcggtacaaa gcagttccca cggcattatg ggcacgattt
    11821 tgtcgctggt gccgacgaac attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca
    11881 tcttcttctc ggtgctgttt ggtctggggc tttcctccct gcccgcgacg catcgtgaac
    11941 cgctggtgac cgtgttccgc tccatctctg aaaccatgtt taaagtgact cacatggtga
    12001 tgcgttatgc accggtgggt gtgtttgcgc tgattgcggt gacggtggct aactttggtt
    12061 tctcttctct gtggccactg gcgaaactgg tgctgctggt gcatttcgcc attctgttct
    12121 tcgcgctggt agtgctggga attgtggcgc gtctgtgcgg gttaagcgtc tggatcctga
    12181 ttcgtattct gaaagatgag ctgattctgg cgtactccac tgccagctct gaaagcgtac
    12241 tgccgcgaat tattgagaag atggaagcct acggagcacc ggcgtcgatc accagcttcg
    12301 tggtgccgac cggttactct tttaaccttg atggttcgac gctgtatcaa agtattgccg
    12361 ctattttcat cgctcagctg tatggcattg acctgtccat ctggcaggaa atcattctgg
    12421 tgctgacgct gatggtgacc tcgaaaggga ttgctggcgt gccgggcgtg tcgtttgtgg
    12481 tgctgctggc aacgctgggt agcgtaggta ttccgctgga aggcctggcg tttattgctg
    12541 gtgttgaccg tatcctcgac atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg
    12601 cggtgctggt gattgccaag tgggaacaca aatttgaccg caaaaaggcg ctggcttatg
    12661 agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca ataattgaag attgccgggg
    12721 ttatccaccc ggcaatgtgt ggatgcctga tgcgacgctg gtgcgtctta tcaggcctac
    12781 cttaaccgtt ggttaatttt tcaaactctt cacacccggt atcaaaccct tccatacagc
    12841 tcaggttcaa ccagtgcagc gccttttgtt tgttcttctc gataaaacct tcctcaccgt
    12901 ttaagaacat cattcccgcc cagtactcgg aataaccggt tcgggaaatt gcagagctgc
    12961 gtttgaaata ccaggttgcc ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa
    13021 tcaaaccaag cagcatttgg gcatcgacgg cggagtcatt ctccagatct tccgaggcgt
    13081 tttctaacag cgaaatcgcc tttggataat ccggtttacc cgcttgagta tttaccagaa
    13141 tatgcgccag cgttacttca ccttctttac tgcccgcttg cgccgctttc tctgcaagaa
    13201 cttttgcttg tggatagtcc agactgaccg gattggtgat tttgatctgc gccagcagcg
    13261 cgcaggcatc agcatcaccg ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca
    13321 aatcgccgga gctaaaccag ctgtcggcaa gaaaatattg tgcgcgtcgg tccccggcct
    13381 ctgctgcctt taaatactgg ctgccaggct cggagtcgtt cgcgtgggca aagaatgtca
    13441 aaaacaacat caatgcgata atttttttca ttttgataat acgattgggt agggagtaac
    13501 cagtataaag agttcgaatg gataaaaaaa cagcctccga aaggaggctg tagaaaggac
    13561 tgtattacgc cagtgccgct tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca
    13621 cgtactgctc ggcggcgcgc tgatcggcga tcggctcgac gcgcacggcg cagtatttgt
    13681 attccggcgt tttcgtaatc gggcttaagt tttcggtaac cagctcgtta caggcaccaa
    13741 tccaccactg gtaggtcatg taaatcgccc ctttgttcgg acgatcgctg acctgcgcac
    13801 gggtgataat tttgccttta cgcgagtgca cccaaaccaa tgcctcatct tcaataccca
    13861 gacgtttggc gtcttcggta ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca
    13921 gtgccgcaca gttaccggtc atcgaacggc aagagtagtg accaacttca cgcaccgttg
    13981 acagtaccat cgggtactcg tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga
    14041 agaactgtgc cagaccgttc ggggtatcaa acttctcttt aaacagataa gaagtcccct
    14101 gatcggcatc tgaagtatcg cggcaaggcc actgaatgaa gcccagttcg cccattttct
    14161 cgtaagtcgc accgtagaaa tccgggcaca gatgacgcaa ctcatcccag atctcctggg
    14221 tgttgttgta gtgcatcgga taacccatac gggtggcgat ttcactgatg atttgccagt
    14281 ccgttttcag atcccatttc ggttcaactg ctttgaagaa acgctggaag ccacggtcag
    14341 ccgcagtaaa cacgccttca tgctcgcccc acgacgttga cggcaaaatg acatccgccg
    14401 ccgacgcggt tttggtcata aagatgtcct gaacgataac cagttccaga tcttcaaagg
    14461 ctttacgtac tgccgacagc tccgcgtcag tttgcagcgg atcttcgccc ataatgtacg
    14521 cggcacgcac ttcgccatgc gctgcgcggt gcggcagctc gctgatgcga taaccggtat
    14581 gagcaggcag gctttccacg ccccaggctt tggcgaattt ctcgcggtta gccggatctt
    14641 tcacgtactg gtatcccgga tacgtatccg gcagcgcgcc catatcgcag gcaccctgca
    14701 cgttgttctg accacgaacc gggtttacac ccgcatgcgg cttaccgagg ttaccggtca
    14761 gcatcgcgag gctggtcaga gaacgcacgg tttccacgcc ctggtagaac tgggttacac
    14821 ccatgcccca caggatggcg gcgcttttcg cctgggcata catccgtgcc gcctgacgaa
    14881 tctcactggc gctgacgccg gtgatatctt caaccgactc cggcgtgtag ccttcaacga
    14941 ttttacgata ctcttcaaag ccttctgtac gggaagcgac gaacgctttg tcgtacagat
    15001 tttcttcaat aatgacatgg cccatcgcat tcaacagcgc gatgttcgag ccgtttttca
    15061 gtgcaatgtg catgtcagca atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa
    15121 ttttcgcccc gttacgttta gcgttaatta cgtgattcgc cacgattggg tgggaatccg
    15181 ccgggttgta cccgaaaacg aacactaaat cggtattatc aatttcgtta atagcattgc
    15241 tcattgcgcc attaccgacc gattggtgca gacctgcaac cgatgggccg tgtcagacac
    15301 gagcgcagca gtcaacgtta ttggtaccaa taacggcgcg cgcaaatttt tgcattacat
    15361 agttggtttc gttacccgta ccacgcgagg agccggtcgt ctggatggca tccggaccgt
    15421 acttctcttt gatggcgctc aggcgctcgg caacgtaatt cagtgcctca tcccaggaaa
    15481 caggttcgag tttgccgcca cgctggcgac ggatcatggg ggttttcagg cgcggggtca
    15541 ggatctgggt atcgttaatg aaatcccagc cataataacc cttcagacac agggtaccct
    15601 ggttggtttt cccctgcgct gcctccgccc ggacgatttt gccgttatcg acgaccaggt
    15661 tgattttgca acctgatgcg caataggggc aaaccgtgac gacttttttc atcggtctcg
    15721 ctccagttaa tcaaatcacg catacgcgct ctcgactaca gtatgcatct tttatgccac
    15781 attttatttg gggtcattcc ctgatattac gggcactatt tattcaaaac tctgacgaaa
    15841 aacaggctgt cgtcagtttt gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg
    15901 gcagacattt tattttttct cgacgacggg agccgcctga tacccgccgc ccagcgattt
    15961 catcaactga atgctttgga tcacccggcg gctgtccagc atcagtaatg acatctcttc
    16021 ggcaagtact ggcaaccggg cttcagtggc ctgtaagcgg ctggttaagc cgcgctgata
    16081 ggcggcctcg gcagcgcgct gggtgaagcg cgttgcttcc acgcgttcag cctgcatttc
    16141 tcgctcgtcg ttgagcgttt gcagacgcgt gccgttgacg gcaacgtcac gcaccgcgtt
    16201 cagtactgac tggttgtaac gttcaatcat catgttgctg gcggcgcgcg tgccttcgag
    16261 attggcattc aaccgtccac cgtcaaacag cggcaatttc agacccggga tgaagttgaa
    16321 ctggcgactg gtttttttga ataaggtatc caggtggatg gcatcaaggc cgaaaaacgc
    16381 tttgatatca aagctcgggt agaacagcgc ccgcgcggaa tccacctgat ctaatgacgc
    16441 ctgaacatac cagcgcatgg cttgcagatc cgggcgtctg gcgagcaact cataagagag
    16501 tgtcgccgga atgccggtct ggactcgtgg taatgccacc ggtttgatct ccggcatatc
    16561 gctggccccc gcgccaatca atgcacgcag agattctcgc gtttcggtga tttgcccttt
    16621 gacggcagca atttgtttat cgaccgccag aatctgtgcc cgcgcgccct ggaaaggcac
    16681 ttgtgcttcc agaccgtgcg ccactttact ctggtgcgct ttcaccgcgt aatcaatcac
    16741 atcgcgagtt tgttctaaca gatcgagcat ctgatagctg gcctgcatac tgtaataaag
    16801 ctgcgctacg cccgtggtca gcgatagctc tactgctgcg gtttctgcca gcgtggcatt
    16861 atgcgcgcca atggcggcgg caaccgctga gcgatgcaca ccccacaaat caagatccag
    16921 tccggcaaac aaagctactg tggcttccgt atagtacggc ccgtccatac ccagtgcggg
    16981 cgcatccatc gcataagggc ttaaaaagcc gttcgccgag acgcgttggc ggttgagcat
    17041 ccctaacgct gcgacctgta attgtgaacc ggcatctaac aaatcggcct gggactgcgc
    17101 tttttcttcc cgcagtttcg cttcggcgag ggtgtgtgaa ccacttagcg tccgttggag
    17161 cagcgcatcc agctgcgggt cattgagttg tttccaccac tgcgcctgcg gccagccgga
    17221 gctggcaaga tgaatatcgt cggccagttt gatttgttcc ggtttgagct gttgatgagg
    17281 tgcagaatcc ttacgtacca gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct
    17341 gcacaacagc agacgtgaaa gttgacgatt gatcattgcg tggctccttg tgcctgttcc
    17401 gtggcgggcg ttaatgccgg ggctgtccag tctggcaagc gggcgaaaag ctggcagacg
    17461 tgttgctcct gttttaataa ggtgggcagg atggcctgcg aggcgggtgt ctcttcaagc
    17521 gttatttgag gagaacggct gagtgcggtt gccagaccgg cagcgtattt ctccagggcg
    17581 tcggcaaact gagctgctct gtccggctgt agtgcgttat ccagcgcctg cgccgagttc
    17641 caggtggcat cccaggcgtg aagaatatcg cggccctgac gaataaccgt ttgcgaacgt
    17701 tcaatcagta aggcgcgttc ttcgctgtcc agttgacgct ccagcgccac gcgttggcac
    17761 atctcttcac aggcattaaa cgccgcatgc agaccgatac gaatttgcag ataagtgcgc
    17821 agagccgtga cttcctgctg gcgtgggatc cgcattactt tacttaacat acccagcgtg
    17881 ccagccagtt tttgcggcag tgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc
    17941 accgcggaca ccacggtacc aatgatgata cccagggcgc gatcgcgaat ttccaccagg
    18001 tcgtacactg ggccaaaaac gttttcgagc gtggcgagcg cgaaggtgac caccatctgt
    18061 gtgccgatat aagaagagcg ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc
    18121 ggtgccagca caaacagcaa ttcgacaata ttgtccagcc agggcatgac cagtagcgtg
    18181 aataacagcg ccagaatcgc gccgcaaaag gccccgccaa aacgcagcac catcttctgg
    18241 tacgacgaac cgacatttgg gttagcgacg atcacgcatg tcagcataca ggtgtgaatg
    18301 ccttcccagt ccacgccgct gtaaaaggtg taacagatca aacaggcgag cagcgttttt
    18361 accgcgtagc gcatatagtc tggattggta aaagcatcgg cggccattga tggcggtttg
    18421 gctgcgggcg ttggcggcgt attcgggtcc atctgaccca gttgtaacaa cgtctggcag
    18481 atattctcca ggttacattc ccgtgccgcc atcgcttcac tttcactgat ccgccagtcg
    18541 ctttgccagc actgaccttc agcaacggca tgctgcagct tattgatttc tgaggctaat
    18601 ttttgccgaa attcaataat tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc
    18661 agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt gccaccatgc gctttgagtt
    18721 cgccagttgg catcgtccgc gagacaaaag acattcagtt tttgcagcgc cagcgcctct
    18781 ctttcaatcc gcgtttcggg tagcggtgcg aggctgtccg tcaggtggct aatggcatca
    18841 tcaagccgat cattaagcgc ctgatgcatt tgcgaaatgg cacgactggg aaaccacagc
    18901 acgccgatta acgtcatcag caaggttgga taaaggccaa caacgataca ccacagcgtt
    18961 aagcgcacga ccacttccgg atagtcgagc atggcgggga aggtttgccc gtaaatggcg
    19021 acaatggcga ctgcgaaaaa gaccagtccc agccgatggg tgcgcatcaa aaacatacag
    19081 cccatcagga tcggcccggc gatgaacaat cggatcaacg gttcgccgta tgaccatttg
    19141 tagatcaaaa acaggctgcc gatctccagc atcgtggcga ccacaaataa gatcgcgaca
    19201 aatttggtgt aaaacgcgtt cgactgaata ccgtaaaaca gcactgccag cgataacgcc
    19261 acgaaaggga tctcaaaggt catcgagatc agaatcacca gcaggcagcc tacccagagt
    19321 tgcacggtct gcggcacgcg gcctggtcgc cgctcgctta actcttcatg aaagaacgcc
    19381 agcagcctga ccaccggtaa tggcagggag ttgagcgcgc tcattattgc ggctcaagat
    19441 tagcgactgc cgaagcgccg atgcggaaca tttccgggtc aggtttatcg accatgattt
    19501 tgaccggaaa acgctgggca acgcggaccc agttaataga gcgagaaact ttcggcaggc
    19561 cgcccagcac caggccgccg tcatccggta gcacgccgta gccaatcgaa tccactttac
    19621 cctcgaaggt tttgccgctg tcactcatca ggcgaatcgt tgcgggtgta cctgagcgaa
    19681 tatttttcag atcggtttcg cggaagttgg cgatcacgta ccagtgacga gtgtcgatta
    19741 gggtaaaaat agggcgcatg gcagaagcaa attgcccgac ggaggttttg agggaaatga
    19801 cccggccatc aaacggcgcg cgaacggtcg ccatttccag atgcagtttg gtcagggcaa
    19861 tatccgcttc gaccgccgca cgctgggcaa ctaatgcatc cacgccgctg acggcgctgg
    19921 cggctgactg cgcctgcaac aatacagcat taagatcggc ttctgcagcg cgctgcgcgg
    19981 tcctcgcacg gtcaacgtct tccgctgaga caaaaccttc tttcagtaat ggctcggtgc
    20041 ggcgtaatgt atctgtggcc tgtttcgcgg cggcacgggc tttttctacc gtggcgttaa
    20101 ccgagtcggc accaaactgt tgcgcgtcaa cgctacgctg ggtgagcata atttgcttat
    20161 ccagcgccgc gagggaggct tcagctttcg ccagattggc ttcgtacggg cgcgggtcaa
    20221 tgcggaacag caaatcgccc tgtttgactg cctggttgtc ggtgaccgcc agttctacaa
    20281 tgcgaccgct gacttccggc accacatcaa tggtatctgc tgacgcgtaa gcgtcattag
    20341 ttgatggcgc actgtctacg cgccagataa cgaaaacaag ggcaaccagc gccaacgcaa
    20401 ccactaacag agcagggaat ttactgcgag gagctttttt cggcgtactt tccatgagcg
    20461 cgtctcaaat agattagaaa aatgccagcc agaacagcat ggcgtagaga gcaaaaaggg
    20521 cggtataaat aattccgaca aatgccagat tgatatttgc ccgctgaata actcgcctcg
    20581 tgatgagcat caaaataagg ctggcaatgg cgcagaaaaa ccagctggga taataagcac
    20641 cgatcaccgg gattgccgga gataatgagc atcccgaaac catgaagacg ggaattatcc
    20701 aggctgtttt tttgagttgc atcgccatgc gagagagaac tgttggcata ttaaatacat
    20761 cctgtctgct gcgaacaata tctgtagtga atatcaatat ccattgaagg cgtcagtatg
    20821 gctattttct cgatgaataa a
//
LOCUS       DAZFRJ010000068        20715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000068 DAZFRJ010000000
VERSION     DAZFRJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.068"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(112..1161)
                     /gene="yahK"
                     /locus_tag="ACHKUN_002317"
     CDS             complement(112..1161)
                     /gene="yahK"
                     /locus_tag="ACHKUN_002317"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO6660566.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1248..2204)
                     /locus_tag="ACHKUN_002318"
     CDS             complement(1248..2204)
                     /locus_tag="ACHKUN_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6660567.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(2201..3172)
                     /locus_tag="ACHKUN_002319"
     CDS             complement(2201..3172)
                     /locus_tag="ACHKUN_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6660568.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(3165..4649)
                     /locus_tag="ACHKUN_002320"
     CDS             complement(3165..4649)
                     /locus_tag="ACHKUN_002320"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6660569.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(4698..5681)
                     /locus_tag="ACHKUN_002321"
     CDS             complement(4698..5681)
                     /locus_tag="ACHKUN_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6660570.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(5935..6345)
                     /locus_tag="ACHKUN_002322"
     CDS             complement(5935..6345)
                     /locus_tag="ACHKUN_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO6660571.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(6644..8026)
                     /gene="yahJ"
                     /locus_tag="ACHKUN_002323"
     CDS             complement(6644..8026)
                     /gene="yahJ"
                     /locus_tag="ACHKUN_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO6660572.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(8036..8986)
                     /gene="yahI"
                     /locus_tag="ACHKUN_002324"
     CDS             complement(8036..8986)
                     /gene="yahI"
                     /locus_tag="ACHKUN_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO6660573.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(9129..10547)
                     /gene="yahG"
                     /locus_tag="ACHKUN_002325"
     CDS             complement(9129..10547)
                     /gene="yahG"
                     /locus_tag="ACHKUN_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6660574.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNITIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(10547..12094)
                     /gene="fdrA"
                     /locus_tag="ACHKUN_002326"
     CDS             complement(10547..12094)
                     /gene="fdrA"
                     /locus_tag="ACHKUN_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6660575.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(12084..12947)
                     /gene="yahE"
                     /locus_tag="ACHKUN_002327"
     CDS             complement(12084..12947)
                     /gene="yahE"
                     /locus_tag="ACHKUN_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6660576.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(12987..13592)
                     /gene="yahD"
                     /locus_tag="ACHKUN_002328"
     CDS             complement(12987..13592)
                     /gene="yahD"
                     /locus_tag="ACHKUN_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO6660577.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            13850..14347
                     /gene="yahC"
                     /locus_tag="ACHKUN_002329"
     CDS             13850..14347
                     /gene="yahC"
                     /locus_tag="ACHKUN_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6660578.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            14439..15371
                     /gene="yahB"
                     /locus_tag="ACHKUN_002330"
     CDS             14439..15371
                     /gene="yahB"
                     /locus_tag="ACHKUN_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6660579.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(15413..16501)
                     /gene="pdeL"
                     /locus_tag="ACHKUN_002331"
     CDS             complement(15413..16501)
                     /gene="pdeL"
                     /locus_tag="ACHKUN_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6660580.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(16643..20626)
                     /gene="ehaA"
                     /locus_tag="ACHKUN_002332"
     CDS             complement(16643..20626)
                     /gene="ehaA"
                     /locus_tag="ACHKUN_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO6660581.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         4995 a         5677 c         4805 g         5238 t
ORIGIN      
        1 tttacgttat aggttttact gctactttga tattatttcg ctctccttaa ctaaaaagtt
       61 aagtggagag cgaagttaat atgttaaacc acagcgtatt tattaatttt ttcagtctgt
      121 tagtgtgcga ttatcgataa caaaacgata tttcacatca ccacgcagca ttcgctcata
      181 ggcttcatta atttgatcgg cccgaatcat ctctatatca gccacgatgc catgttcggc
      241 gcaaaaatcg agcatctcct gagtttctgg aatgccgcca atcatagaac cggctatcgc
      301 acggcgtttc atgatcaggt tgaaaacttc cggcgattta tgcggtgtcg caggcgcacc
      361 aaccagcgtc atggtgccat cacgcttcag caaggtggta aaatcgtcga gattatgtgg
      421 cgcagctact gtattcaaaa tgaaatcgaa actcttcaca tgagccgcca tctcatcggc
      481 attgcgtgag ttaacaactt catcggcccc cagggctttt gccgcttcgc gttttgcctc
      541 agaagtggta aatgccacca catgtgcccc catcgcgtgg gccagcttaa tccccatatg
      601 tcccaggccg ccgatgccga ccacgcccac ttttttaccc ggcccggcct gccagtgacg
      661 tagcggcgaa tacgtggtga tccctgcaca caacaaagga gccaccgccg ccagctgctc
      721 ttgcgggtga cgaatacgca gaacatatcg ctcatgaacg acgatctgtt gtgagtagcc
      781 gcccagagta tggcccggtt cgtccggcgt cggcgagtta taggtgccgg tcatgtgatc
      841 acagtagttt tccagcccgt cttcacactc ttcgcaatgt ttacaactgt cgacaatgca
      901 gccgacaccg accagatcgc ccggcgcatg tttttctacc tgatcaccaa cggctaccac
      961 acgccccaca atttcatgac ccggtacgca ggggtaaacc gtccccgccc actcggaacg
     1021 gacctggtgg agatcggaat ggcagacgcc acagtaagcg atttcgattt tgacatcatt
     1081 cggtcccggt tcacgccgtg tgatatccat cggttcaagt ggttgtttag cggaatatgc
     1141 accaacagct ttgatcttca ttgtgtttac tcctgattag ctatgtgtat ttggcgatgc
     1201 ctgatatgtc atcgcggcaa aacgcgtcca ttgaatatag ccaatattta aaccacccga
     1261 tccagcccca acatgccgcg aacctgaatg aaacagagca gcaacgttcc ccataaggcc
     1321 atcgtcaggt aggggcttat accgagaata ttgaagccgc tttcaataat ttgcagaaga
     1381 aacagcgcca gcaccatacc aattatccgc ccgctgccgc cgtccggatt aaccccgccg
     1441 agtactgccg cgaggattga caccagcaag tacgattcgc cataagaggc cttagccgag
     1501 ttgagtttcg acatcatcaa aaatgccgct accgcacaaa gcaaggcgga aatgacataa
     1561 acccaaatca gcacccgacg agtgttaatg ccggagtaaa gtgttgctcg ttcgttagac
     1621 ccaatcaggt aaatggcttt accgagcggc gttttctcca gcaatatcca cagccccagt
     1681 gccactgcgg caaacaacca catcggtagc ggaataccga accattgcgc atgattcaac
     1741 cacagcaccc atgagggata attggcaatg gcgctaccac ctgtcaccag aatatttatc
     1801 cctttgagca acgtcatcat cccaagcgtt gcaagaattg gcgacactcg aataccagca
     1861 atcaatacgc cgttacataa accgattatc accgccgcgc cagcgcccgc cagtaacgta
     1921 gcgacaaccg tagcaatacc cggtggatac tgagtcgcca cccacgccat caccagtgaa
     1981 caggcgttag cagtggctat aattgacaag ttaatgccac cgcaaagcat ggtcaccgcc
     2041 atagccaacg ccagaatgcc cagcacgggc atttgcgaag cgacggactg gaaattactt
     2101 atcgaccaga aaatctccgg catagtaatg ctgaacgcgg caacagttag caccagcagg
     2161 ccgatcaagt aaaactcaac gttattacgc cacgattttt tcatagcaga ctccggttct
     2221 gatgctgact ccacgccgtg gcactaatgc tggcaacgat gatgatgccg gtgatcaatg
     2281 tttgccagta agacgagact cccaataaat ttagcccgtt ttgcatcact gccaacaaca
     2341 ccacgccgag caaagtaccg gttaacgtgc cgcgcccgcc gagcaaacta gtgccgccaa
     2401 gcaccaccgc agccagtact gtcagctcat aacccagaag agaatcgggg gcgacagtca
     2461 tcaccgtcca cgactgcact acacccgcag cgccagacat caatcccata tagccgtaga
     2521 cgaaaagttg cagtttcagc acgctaaagc cgatgcgact ggctgattcg cggttcccgc
     2581 caagggcata aattttgcgc cctacactgg tgaaattcat gataaacgct gtcagcagca
     2641 ccaccgtaat catggcgatc agcggcagac caaggccata gtcatagcca tcagcatcgg
     2701 tgtacttgaa caacataacc ccctgctcaa accacggcgg aaagtcgtac agccacacac
     2761 ctttactcaa ccataacagc aggccataga aaatattcag cgtcgagata gtgatgatga
     2821 tagaaggcac ccgtaggcga ttaaccagta aggcgttaat taagccaagt aaaatgccga
     2881 tgcagcccgc cagtgcaaac gcgacagcga agttgccacc aattttttgc agcaacagca
     2941 ccatgccgta ttgcgcaatg atggtcattg ctggaaacga aatatcaatt ccaccggaaa
     3001 tcaacaccac aaacaaacca caggcgagaa tagtcaacat ggcatagtta ttggcgagat
     3061 cgtacaaatt accgaatgtc agaaactcgt cgctgcgcca ggcaaggcca acaaacagca
     3121 ccacaatcag taatgccagc cagaattcat ggcgtttttt cagttcagcc atttaccacc
     3181 tccgcaatcc tggcctgaga actgctgtca ggcaggaagc tatgggtgat ctgacctttt
     3241 tgcattacca aaatccggtg gctttgatac cacgcttctt caatttcatc gcagatcatc
     3301 aataccgcaa tgccatgagc cgccagatcg ctgataatgc cgtatattcc tgctttgttg
     3361 gcgatatcaa caccgactgt cgggctatcg agaatcaaca gtcgtggccc aatcgccagc
     3421 catttggcga tcgatactcg ctgcgcgtta ccaccagaaa gcgtattcac cggaagatga
     3481 ggatcagaga ctttgatggt caatgccttg attagcctgt ccaccaggtc acgaacttta
     3541 gtttcactca gaaagccgaa gcgatctttt accttgtgaa aaacagtgct aatgatgttg
     3601 tcttcaatag attgcgccat caccaaaccg cgcgacatgc gatcttccga aacataacca
     3661 ataccgtggc ggatagcgtc ctggttacta tgcagtgtaa ccggctggcc gttgatgagg
     3721 atctcgccag catccggtcg ggtcatacca aacagactca gacataattc cgtgcgcccc
     3781 gcaccaagca ggccaacaat cgataccact tcgcccgctt ccacccgcaa gttaatattt
     3841 agatattccc catgacgact taaattacgc acctcaagca ccggggtgct ggattttccc
     3901 tgccacaact ctcgcacctg atattcgaac tcctggccgg tcatcaggaa accgagttgt
     3961 tttgtggtca tctctgcagc cggaaaagtc ccgaccagct caccatcttt cagcactgaa
     4021 atacggtcgg aaacttccat cacttcttcc agacgatgac tgacgaagac cacgcagatc
     4081 ccgcgttccc gcaattgatg caccacctgt aataaccctt gcacttcctg atgggtcagc
     4141 gaagcggttg gttcatccat cacgatcaac cgtgcgtcct gcgccagtgc acggcaaatt
     4201 gctaccagct gacagcgggc aattgaaagc tcagaaacga gcgtatccag tggcaatgtg
     4261 acgttaatac tggtcattgc cgcctgcgcc acctcacgca gacgacggcg gttaacaaac
     4321 agaccgtggt gatagtgatt cacggcgata ttctcccaga cactcaggtt aggaaataaa
     4381 gagaggtcct gataaatcac ctgaatcccc tgcgccaccg aagcggcggg tgtcagcttt
     4441 gaccagtttt tcccaccaag cgtaatttcc gctccctcat ccgggcgata aacaccggac
     4501 ataattttaa tcaacgtcga tttaccacag ccgttttgcc cggcaaggca gtgcacttca
     4561 cccgacataa agtcgagaga aacatcgcgc agcgctcgcg tggcatgaaa cgttttgttg
     4621 atatgacgaa gggaaaggaa ggtttccata acatcccctg gcaataagaa tcggggcttt
     4681 cgccccgaag aaatggatta ataaagtgag tcgatattgt ctttgttgac cagtaatacc
     4741 ttatggaaac ggataattcg cttatccata tctacgtcgg ccttacccag attctgcatt
     4801 tctaatccag gtttgatctc ttcacccttc agcaacgtgc tggcgacggc tgcgagtgcg
     4861 taacctgcag aggccgaatc gtaagtaatc ccttcggtga tatcaccact tttaatcagt
     4921 gatgccgcct gtgaagggat catcatgcca tagactgcga ctttattttt cgcccgtttc
     4981 tctttcaccg cacgtcccgc gccaatcgga ccgtttgaac caaaggagac aaccgctttc
     5041 aagtcaggat aggttttcat caggtccagt gtggtacgac gtgagtcatc cacactctcg
     5101 gcaaccggca tgcggcgggt aacttcatgc atatccgggt aatgctcttt ctggtatttc
     5161 accagcaagt cagcccataa gttatgctgc ggcacggtca aactacccac gtaaatcaca
     5221 tagccgccct tgccccccat gcgtttcgcc atatgctcaa catattcagc ggcaaatttt
     5281 tcgttatcaa tgatttcgat atcccagtta gcacttggct gaccggggga ttcgttggtc
     5341 agaaccacaa ttccggcatc tcgcgctttt ttgaataccg gttccagcac gttggcatcg
     5401 tttggcacga tagtaattgc attaacctta cgggcgatta aatcctcaat aattttaact
     5461 tgttgcggag catcagtact tgaaggcccc acctgtgacg cattaacacc aaaggcgtta
     5521 cccgcctcaa ccacaccttc gcccatgcga ttaaaccacg gcataccatc gactttagag
     5581 atattcacca cgactttttc cgctgcctgg agcggcgcag aaattagcgc agcgcctaat
     5641 aacagcgaag acaccatatt gataacaaaa cgtttattca tcatttcagc ctcattgcag
     5701 ggtagtcagg aagtgttcgc ccaaattagc gcgctggaga gaataagaat tttcgaatat
     5761 ttttatgcag aataaacagc cctatttcag gcagctatta aaaatatggt tataaatatg
     5821 gacaaaacgg gcagcaacaa taagtgtgac attcatcacg taaaaaatag caaaataaac
     5881 aaaattagcg atattttatg tgtgtgagtt tatttcgcgt agataaatag ttatctacac
     5941 aacgtcaata agggcatatt cttcaaatat aacgtccatt tctggtgaat agcttccttc
     6001 tgcctggaaa aaacgacggt gttcattacg ccaatattca agactgaggt caccttcacc
     6061 ttccttacgg gcaagctcca cggtgacatc agaaaagcgc agtaaatgca ggccaatcac
     6121 tctgataaca catacaggct caccacggct attttctaca atcttatact caccaatcat
     6181 cggaaatgcg ttgtcttcaa tacatcccgc cagcgaacca caggaagctg ttttaacacc
     6241 agtcgcaatc aacgttgcga ggtgatcggc caacgctgct gaatcgccaa aagtacaaaa
     6301 ggaagcatgg ggatattttt gtgagaattt ttcttttatg gtcatggcaa tatttttgcg
     6361 attacggaaa aacaaatcac atcttaatta ctgttggtaa ccatcagcaa gagtatcatt
     6421 ggtttttcga tactgaatct tttttatccg aagcaggtca catttatggc ctgataaata
     6481 cctttgttaa aactcacttt tgtgtatttc tctcaaataa tcccctggaa attatgccgc
     6541 gagattataa agttcaggat cattcattag caaaccattc ccgccccact ccctggggct
     6601 taaatctctt acaattcacc gttgcaggcg tttcaaatgc gcatcaacct gccacactcc
     6661 cccacaccag ttgcccttta tggaacgttg cggttctcgg cgagatacgc gccaccgcct
     6721 cggcggaaca ggaggcgtcc accagcacaa agctggcatc atcctgcgct tttggccata
     6781 cacgctcgcc tttttcattc agcggcaata catcgccagt ggctagaaac agcgaacggg
     6841 agaggttctg ttcgttagga cgaatataga gctgcgcgta gagattggct ttttccagca
     6901 tgtcgcccag accataaggc gaccagtggt cgataacgct gtcagtgcca gtcatcactt
     6961 ttacgccttt gtcgtgcaac tgtttgagcg gcatatgcag cgtgccaatc ggcaccgtcg
     7021 aggcgataga aatttgctgc gccgccatcc gattcgccag cgcatcgacc tgttgttcgt
     7081 tgagcgtagc cagcgcaaag gcgtggctga tggtcagctt acctttcagt tgcggcgttt
     7141 tctctaccgt ttcaaccata taattgatgg ctgccacgcc cgacggagtg gtttcgtgca
     7201 ggtgaatatc gacacctttg tcgtagtcca gcgcaatctg aaacatggtg tcgagggatt
     7261 tttccatcgc gccatcgaca ctggtcgggt ccagcccgcc gacgtaatgc gcacccgcct
     7321 gcatcgcttc gcgcattaag ggttccgatt tcgacagcag caaaccgtgc tgcgggaagg
     7381 cgacgatttc acactcgaag cccgcctgac gtcgcgccag caccgcctgc aaattctgca
     7441 gatttttcag gccggaaacc ggttcgatat tgcagtggct gcgggcaatg gtggtgcctt
     7501 tcgactgcaa tagatcgatc agtttttctg cccgttcctg cgtgtacggt tgcagttccg
     7561 gcagcatttt ctgctcaagc ttgatcatgt cctggatagt ggtgcctgcc ggacgattga
     7621 gcgagcgcca cggcccgccg tagaaggttt tgtcgagatg aatatgcatg tcgcgagtgg
     7681 tgggtagcat caacttaccg ccagcgtcat agtgcggcag cgtggcgtcc ggatgctgct
     7741 tgttctcgcg cagggcgaca attttgccgt cctgaatctc cacggtctgg cgcgccgtgc
     7801 gggtctgcac cgccacaccg ttttcgtagt caaaaccggt ttccagcagc acgttatcga
     7861 gatagtaatg cgggtcagtg attttcatgg tgttgttcgc ttcgcagttt agggtgccag
     7921 ctaccgccgc atgggcgagc ggcacagagg taccaaacag cgcggcggcg gtgaccatct
     7981 taccgctctg gctcagaaat tcacggcggc tattgctttc tttcatccta cgtccttacg
     8041 ttttaataat atgggtgccc gtttcaccgc gcagcgccgc aggcaggcat tccggcgtgg
     8101 tgataatcac ttctttgccg ccctgctcca ggaacgtcag gctggcgatg atttttggca
     8161 acatgctgcc gggcggaaaa tgcccttcct gcatatagcg agtcatggtg gcaatatcca
     8221 cccgatccag cgcctgctgc tgcggtttac caaagtggat acagactttt tcgacgccgg
     8281 tggtgatcac cagaatgtcg gcgtgaattt cacgggccag cagcgcggta gagagatctt
     8341 tgtcaataac cgcgtccacg ctttggtaat cccccgcttc agtacgcact accggaattc
     8401 caccgccgcc cgcgccaatt acgacaaaac cttgttggat cagcgcctta atggcgggtg
     8461 cttcgacaat acgtttcggt tccggcgagg cgaccacgcg gcgatagccc cgtccggcat
     8521 cttcaacaaa acaccagtca gggtttgcct tttgtagttt gtcacgctgg ctttcactga
     8581 agaatgcacc aatgggcttg gtgggatggg caaaacctgg atcgttttta tccacttcca
     8641 cctgagttac cacggtgacg gcttttttct cgccgtgacg cgccagccgg ttattcagcg
     8701 cctgttggat cagatagccg atgccgccct gcgtatctgc cacacagttc gccagcggcg
     8761 ttaagggcag cccttcgcgc tcgtgggcaa tctccgcacg gcgtaaatcc agcccgacct
     8821 gcggcccgtt accgtgggtc agcacaatgt cgtaatcgga agccagcatt tccaacaccg
     8881 tatcggccac ggctttcacc gcctccgcct gatgctcaat cgactggctg gcgttatctt
     8941 tgataatgct gttgccacca atggcaacga ccacaagctc tttcatgttg ttatgtccgc
     9001 tacagagggt gattaggtta tgaccaacgc cagactgcct gatgcgctac gcttatcagg
     9061 cctacaattc tcgggccatc cgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     9121 catgtaggtc agacgccgta acgttcgcac cagccaagaa tggctttctc gaagcaggca
     9181 aaaggtggat gcacaatgcc cgcgccaatc atgccgacgc ccgcatcttt atgggcgatg
     9241 gcagtgttga tcaccggcag aatgccgctg ctacccacgc gggtgatgtc gattgccgac
     9301 ggcacgccca taaaaccgag cagcggaatg gtgatgttgg ggttttcacc gagagtgatt
     9361 tcacgcatct ggcgagagaa atcgatagcc tcttccaccg tgccgcccac cagcgcaacg
     9421 attgcaggcg cggtcgccat agcaaatccg ccaataccgt aggtttcagt gatggcgctg
     9481 tcgccgatat ccagccccga atcttccggc ttataaccgg caaacatcgg gccgatgacc
     9541 tgctgcgccg gaccggtaaa ccattgcccc ggtaatccgc tgacccgcag gccgaactcg
     9601 acgccgttac gcgccatggt ggtgaccacg gtgctgtatt cgatgccgtg cgccgcatcc
     9661 atcgccgctt tacacatcgc catccacgtc gggccggaga agtagtcgct gctagcgaca
     9721 aactcaaaca cttcgcgctg tttctccacg gaataacctg cctgaataat tcccggcgtc
     9781 agcgcctgaa tcagcaacgt cgtcccggcg ttattgcggt tatggcactc atcgcccata
     9841 tgcagcgcct gcgccagcat taagcgcaga tcaatctcgc cgataatttt catcgcgtcg
     9901 cgcagcattg gcccctgcac atcgcgcatc cagttgaggc ggtcgatcac gctctgatcg
     9961 ttggctccca tacgcaaaat cttcgccatc tgctcgctca tattggtgta agcgatgttg
    10021 ccgtaggttt tgttttttac gatgtgcata aacatcgagg cagaggtaac acccgccatc
    10081 gatcccacac agtcgtgttc gtggcacggc gagaaggtga tctccccgga ggccgccagt
    10141 tcagccgcct cgtcgagatc tttcgccagc ccttcgaaca ccaacgcccc ggtgaccgcg
    10201 cctttcatcg cgccgcacat tttttcccag gtgatcggcg gcccggcgtg aagaatggtt
    10261 ttcggcgtca tgcccggcac cacgttaatc gcctgatcaa aaccaatcag caccggatgc
    10321 gactggataa tgcgctccag cgcctgctgg ttagcggcgg cgattttgtc tgccagcggc
    10381 gaatcggcaa tgttatccag cgcctgcacc acctgcatat tgccctgccc cggcggcgtc
    10441 cagtcgagtt gagtcacttc tacatgctgc tttttcaggt catcgctaaa catggcgatg
    10501 ccgacgttaa taacgttcaa tggttggcta aacagtgact ggctcatcag gcttcctccc
    10561 ctttgcagat aaattcacgc gccagcaatc cggtattggt gctgctgctc gccagaatca
    10621 ctccggcatc aagcagcatc tggctttgtt gttctaacga tggcgtatca agatcggtac
    10681 ccagcacata ggcgagaatg atcaactcgc gtccttcggc agcggcgatc gctttcgctt
    10741 ctttgatcgc ctcgatggtg gagccgaccg gatcttcatg cgatccaaat ccgagcacaa
    10801 aatccatcac gatcaccgcc acttctggat cgcgcgcctc ttcgatcaag cgactgatgc
    10861 ggttggtggg gtcaatcatt ggatgcggct tgccgttcgt gaagtcgtca tcgccaaagt
    10921 cgaggaaggt gtgtttgatg ctgcggttga tatcttgcag gcggaattcc ggatcgggct
    10981 gaatgttgct gtagacatcg ccatgttttt ccatcaccgc gaacatggtt tcgtcgcaca
    11041 gcgtgccgcc gcagaacagg ccacgaatgt atttctgctg cggttgcaga cgcgcacgca
    11101 catcagcaat caacggctgg ttaagcgtat gcaggtcgag attttcctgt ttcacgccgg
    11161 agagcatcac cgcttttagc gccgcctctt tgctgccgcg ggcaaactgt agcccctgct
    11221 catccaccgg cgtttcaacg cgaccgagga agcagaccac caccggcttg cggcaggcac
    11281 gtgcgcgttc cagcactttg cgggccaccg caggcgcggg aggttttgaa atcagtgcga
    11341 taatttcagt ttgcggatcg ttttccagca tcccgatggc gtcgagcatc atcaggccgc
    11401 cgattttctc gctcaggtcg cgcccgccgg tgccaatcag ttgtgaaaca ccgccaccaa
    11461 attcatgaat gcggacgctc agctcctggc tgccggtgcc ggatgcgcca acaataccga
    11521 tattgccgcg acgcacggcg ttaccaaaac agagcgccgc gccgttaata atcgccgtgc
    11581 cgcagtccgg ccccatcatc agcagccctt tttcgtgggc caactgcttg agcgccagtt
    11641 catcttcaac tgagacgtta tcggaaaaca gcatcacgtt gagatcgttt tgcagcgcct
    11701 gacgcgcttc gcgagcggca aacagaccgt tgaccgaaat caccgccagg ttactttccg
    11761 ggatatgctt tttggcgctg gcaatagtgg cgtaacgcgc ctcgtgcgag ccgctttgcg
    11821 ctttggtgtt gaacagttct tcaatctcca ccagcaactg ctcgttgtcc gcacccgatt
    11881 taccattgat gacaatcatc aggtcgccgt ttttcgcctg ttccagctcc ggcgtcagca
    11941 gtcctaaatt tttcagtaca cctttgttca tttcggtcgc catcgccaca aatgcctgct
    12001 cgacgccgtc gagtttattt gcacgcgtgg agatagacat cagcgagaca gaatcaaaat
    12061 aggtattcgg tttaatgact attttaactg acataattcc ctccgtaggt ttggctcagc
    12121 gcacaaccat cggccatgcc atacagcatg tcgcagccgg aactggagcc aatatgttta
    12181 attttttcta ttgcctgagt tgcgttccca gggatgtcat aaataatgtt gtgaataaaa
    12241 cgatgaatat tttcccggca acgttgttgt aatgcggctt ccagcgttat agcacttaat
    12301 aatgtggtgt tatttctgcc gcgctgcata ccgagataaa attcctcttt gtatttttcc
    12361 gacggatgcc cgggaataaa taaaataagc gccagacctg ttaaataatc gtcggctgat
    12421 ggcgtaaggc caatccctaa cccaatcata ctggcgaccg ctgcggcgat attttgtttt
    12481 tcgtttactg cctgaataac tgcccgtcgt ttaatatgta gttctttatt taacgcctga
    12541 taaaacggat tatcgccgcg ataattaaac agggtttcat tttcccccag gtgctgatga
    12601 ataatgtcgt gccactgtag ccaggcgata aggtgaaaat tcacagcggt catttgcaag
    12661 gttggggaca gccaacgccg acaacgactc atctctatat gaagatgttg accaaccgta
    12721 ataccttgat cgtcaaactg aaccttatca ccatgccgga acagatcatc aaagtgagtg
    12781 agtgccaacc gacaactgtt tggcgcattg tcgtactctt cacaaagcag cgtcagcaac
    12841 tgctggcgag ccgggagtgc gatatttacc gctctggcaa agacctgttc aacctgtcct
    12901 tgcccccgct gcgccagaaa atccgcatcc gcagttagcg cccacacaca ccgtctttct
    12961 ggtgtgttga tagcaagcct cccggtttat gcacctgcgg caatcagtaa ctgcgcaatc
    13021 tcttcaaagc cctgttcccg cgccagttcc agtggcgttt tgccatattt atcggtcaga
    13081 tgcgggctgg caccgtgttc cagcaataac tgcacaatcg cctgctgttt aataccacca
    13141 tcattaagca caatcgcttc cagtagcggc gtccagccta catggttggt ctggttaacg
    13201 ttaatctccg tatgcgccaa aagctctttt acaatactta aatggccttt ttcacaggca
    13261 ggcgtcaggc cgacaccgcc aaaacgggtt acgcaattaa gatcgggttt agccggtaaa
    13321 ataattcgta gtagcgttaa atcatcgttc agacagctaa ttaaaaaagg atttaaacag
    13381 gtatgatctt gcttattaat atccgctccg gcatcaatta atgcctgaac gcaagcatat
    13441 tgctgatata aacttgccag cgtaattgcc gttttcccct gacgatcgca ggtattaata
    13501 tcgacaccca gcgcaaggca ggtttttact ttatcgatat caccctgttg tgcagccaat
    13561 aaatagtcgg caggtagatt ttttatactc attcacattc ccctggtttt tgtggctgtt
    13621 ttcgatgaat tgagaatagc aacgataatc tgataaaaga acccgcttaa atttaattcg
    13681 ctgtatttcg cctttttgtt gaatgatgtt caacaatctt ttcgcagtgt gcggtagtgc
    13741 aatatttgtt tctttgaccc gatacccttt cactcaatca ggttttattt ctctatagtc
    13801 tgcacatgta agagtagctt tcgtccatgt ctaaccagag gggaacaaga tgaacggact
    13861 cacggcaacg ggtgtcaccg tcggtatttg tgcagggctt tggcagttag tgtcttcaca
    13921 tgtcgggctc tctcagggat gggaattatt aggaaccatt ggcttcgtcg ccttttgcag
    13981 tttttatgcc gcaggcggcg gtaaatcggg gtttatcaga agtctggcgg ttaattattc
    14041 cgggatggtg tgggcatttt tcgccgcgct ggctgccggt tggctggcct ctgtaagtgg
    14101 actgtccgcg ttctgggcga gcgtaattac taccgtaccc ttctcggcgg tagtggtctg
    14161 gcaagggcgt ttctggttac tgtcctttat tcccggtggc tttctcggca tgacgctgtt
    14221 ttttgccagc gggatgaact ggacggtgac gttactgggt tttctggcgg gtaactgcgt
    14281 ggggataatt tcagaatatg gtggacaaaa attaagcgaa gcgacgacta aacgcgacgg
    14341 gtattaaaag ttattcaaaa atgattatct atctttaacc gatacctcta cagtcatagc
    14401 tggagaaatt catagtagat aatggcgaga atggtttaat gaactcaatt tttaccgaag
    14461 agaatctgtt ggcatttact accgctgcgc gttttggcag cttcagtaaa gccgcagaag
    14521 agttgggttt aaccacttcc gccattagct acaccatcaa gcgtatggag acggggctgg
    14581 atgtggtgct gttcactcgc agtacccgca gcattgagtt aacggagtcc gggcgttatt
    14641 ttttccgcaa agctaccgac ctgctgaatg atttttatgc catcaaacgc agtatcgata
    14701 ctatttcgca gggcatagaa gcgcgtgtgc gcatttgtat taatcagctg ctttatacgc
    14761 ccaaacacac cgcacgcttg ttacaggtgc tgaaaaagca gtttcctacc tgccagatta
    14821 ccgtcaccac cgaagtgtat aacggcgtct gggatgcgat tattaataat caggccaata
    14881 tcgccatcgg cgcacccgat acactactgg acggcggcgg tattgattac actgaaattg
    14941 gcgcgattcg ctgggctttt gctatcgccc cggatcaccc gttagccttt gtgccggaac
    15001 ccatcgccga aagccaactg cgtctctacc ctaatattat ggtggaggac accgcgcata
    15061 cgattaataa aaaagtgggc tggttgctgc acgggcagga gtcaattctg gtgccagatt
    15121 tcaacaccaa atgtcagtgc cagattttag gtgaaggaat tggttttttg ccggattaca
    15181 tggtccgtga ggcgatgacg caatccctgc tggttacccg acaaatccat aatccgcgcc
    15241 aggattcgcg catgttactg gcgactcagc attctgctac gggtcaggtg acgcagtgga
    15301 ttaaaaaaca atttgcgcca aatggcatat taacggggat ctatcaggat ttactgcatc
    15361 gggagaatta atcgctgcca acctcccgat gcgctgtgaa tagcggttta catcaaccac
    15421 ctgctttcat tacccattca gagataaatt tattacccgg tactggcgga gagtacaaat
    15481 agccctgcaa aaagtgaacg cctttaccga tcattaaatc cgcctgctcc tgggtttcta
    15541 ccccttccgc cacgatactc agaccaggct tacgcgctag ttcgacaata ttgtccacaa
    15601 tatgaccgga gatttcgtcg acactcgcca tttgcacaaa tgacttatcg atcttaataa
    15661 aatcgaccgg gaacgcctgc aagtaacgat aggtcgcata acccgtacca aagtcatcca
    15721 gcgcaaaggt aatgttgtgc tgatgaaggc tgtcaaatat cgctctggct tctggcgtta
    15781 ccggaatagg gttacgttcc gttagctcga gaaccagctt gattttatcg ttacctaatt
    15841 tcttaaccag gttcagacac tctttttcaa atcccgctgc caaaaaacaa cccgccgaga
    15901 cgttgatgcc aatatggaaa ttgtccggca gcaaatgttt taccggcatc agaatatccg
    15961 cagtctgttt catcagttgg cgggtcatta tgacaataag accggatgac tccgccagag
    16021 gaataaactg atccggtggg ataattcccg tttgtggatg ttcccagcgg acaagtacct
    16081 cacagcccgt cagtacccca gtctgcgcgc agaaaaccgg ttggatccac ggtttgaatt
    16141 cgtggttttc caacgccaga ctgatggctt caggcgtgac gatatggtga caacggttat
    16201 cagggatata aaaattattt ttatttcccg tgccactgat gacttgcgga tgatattgaa
    16261 aaaagatatc ccgccagaag gtaatatcac tctggatgcc tagtttttca tacagctgct
    16321 tcttttgatg tgaaattgtc tttgcactgc gatttctgta ctgtgagatt tctgtaaccg
    16381 acattccctc cattagcagc ttgagtaaaa gccgttcttt ttcggacacg ataccaggca
    16441 acgtcaaatt caccgttgcc ccgaactctt ccaaaaaaac actcaaatca tgcgaagcca
    16501 ttgaacacac cttgattaaa tcttgtgcgc cagctaaatc tgcgagtctt cactctgact
    16561 gataaagctg gctgcaagaa aatgacacag agagccgaat tatctcccgt tcggctctct
    16621 ctccttttac tcattattca gatcagaatt gccacttaat gccaaccatt gcagaggtgt
    16681 cattatatcc cctatcggca acctgaacgc ccacattccc ccagacattc aggttggcat
    16741 ttagctgtcc ttccaccccg gtttttatct cagcaatatt tcgggctcca tcctgagtga
    16801 cggacacgcc atccatactg gtgctgaaat ccttactgtt atgcagccag ttcacttcta
    16861 caaacggctg gaactcgcgg gatttaccgt catccatttt atggtggctc ttcagccagg
    16921 tttttacgcc aagtcgggtt tgaacattgc catcaccgtt gctatgaacg agggttccgt
    16981 tgctttcgcg gtgcttatcg gctttgactc ccatccaggt aacctgtgct tgcggctgaa
    17041 cataccattc attacgcgtt ccctggctgc cattaaattc agctaatttg tgtttgtatc
    17101 cagcttccag tgaagcggta aatccttttg atttatagga ttcactttgt aagtcatccc
    17161 ctttcactgt gttatcaaac cagctgtact gcgcccaact gtcgagatac gcgccattac
    17221 gcgactcgtc atcggcatac caggtggcat agaggccggt gctatatccg ttcacactcg
    17281 cttttgcacg ataaccggtt cgcgaggaaa tggttttgct gtcgctgttg ccatatcccg
    17341 ccatgacccc aacatgccag ctgtcgctgc cgttttggct ccactgcgcg acatcgcctc
    17401 ccagttgcag aacatagcga ttgctttggg ttttcagctg gccgctgcca tcacgccatt
    17461 tattatgacc accttcatgg cgcatccaca tagtggtttg tttctgctca cccgtcacca
    17521 tgtcggtata gtacgtatta cccagacgct catgcagacg cgtggtgaac attgtattcg
    17581 ctgctgcaag gttcgcaatg tagctacccg cctccggtcg caggtcatta tccacattca
    17641 gatccggacc cggcgtaggt gtcgggttag ggttcggctc tggattcggg tttggctccg
    17701 gattcggcat cgggtctggc tccggctgca gttcaggaga atcactgccg ctggtcagat
    17761 accagttacc actatttgcc ccttgtccac gcgcgagagt gtagtcatac gcccccgcaa
    17821 cgatacgacc agcctgaaca aattcgccct cagacttacc gtctacatgg ataagttcga
    17881 taccgttaag ggttttcgca cctgtaccgc cagcgttatt caccgtaacg gcagtcgtgc
    17941 cgctagtatc gccctcgaca accaatttat cggttactga gtcatcgcca ttcagtaccg
    18001 tattcattac tagttgaccg ttattaccgt ggtagttccc cttcaccact aacacattgc
    18061 cagcttgctg tcctgacatt ccggtcatca gcgtaccgtt gttggtcaga ttgccgccca
    18121 gggtgtaatt cacagacgca gccgctcttt tttcagtgct attattcaac gccagggtac
    18181 cgttgttctc aacgtttgcg ctacccaatc cctgcgcatg acttaccagg acattagccg
    18241 aattagcaat gttcacattc gccgtcaggt tgctgttatc gcccgcgatg tccagcgtgc
    18301 cgccctgaat attcagctct ccgctccccg ttaacgaacc ggttgaagtg cccccgttgg
    18361 taaccgtcag actgccgcca tttaagctca gcagtgaatc ggcgctgcca ttgagcgtgc
    18421 ccacggtctg gctgtgacca ttcatatcca gttcagtctc tgccgccaga cgcagttcat
    18481 gggtattacc caacacgtta tcgttattca acaaaagcgt cccactgcgc agatcggtaa
    18541 caccggtata gtcgttgtct ttgttagaaa gcgatacggt ctgacccttc tggctgctga
    18601 atgccaggtc accactgccg gtaatctttg cgctgagatc ggcggcattc tcgcttttac
    18661 cgttagagct cagcaccaga gcatcgctgt cggtagcgtg taaatcaagc tgggtcaggc
    18721 cgtaaccgat atacagaccg tcattgttta ccccactcgt cagacgatag tcgtaagtgc
    18781 cgacggccgc tttttgccca ttctgctgaa tatcacgttg ggcgctgctg gagaggattt
    18841 gcccattttt atcctgcaat tgcagattgc ccgcatcgcc cagaacttcc gcaccttgcg
    18901 catcaaccag cttaatggcg ctgttaccat catcgacttc agtgagcgat aacgcagagt
    18961 caatatcacg agatactgag cggacaacgt cactgtcaga aacctgaatc acaccctcgc
    19021 cgcgcaaatc cagcgtttta ctaacgttga ccgtcccttc tgtcatctgt gcacctgctg
    19081 tcagggcacc aaaatcaacg gttccgccgg agaatcccaa agtgctaatg gagtgagcgc
    19141 ccttacccac tgttacctgg cttccctgac ccgcagtgat cccgcctctc tgcagaaact
    19201 cagcattctt ttccaggtta aatgtcgtgt tctggaacgc cagtgaaccc tggaaccagt
    19261 ctgtataagc aaactcatcg aaggagaatt cgtgattccc catgtttatt tcaacaatac
    19321 ctctaccttg aagatcgtta tcaaataccc aatctttatt ggcattgatt tgcagtttgc
    19381 catcaacatc caggtccgca cggccgatgt tcttgtcttc agaaataacc gcgacagcac
    19441 cagaagcgac atcaatatat ccgctgaatg catagttatt gccggtcagt gccactgtcc
    19501 cactgtcgat acttattgta ccattgccgg aaatcttatt ggcaaaggtc atgctatcgt
    19561 cagccttcag gtgtaatttc ccgcggttgt tgatctctgc tgtacctata tttttggtga
    19621 tccctaaagc gccattttgc tcaattttga ctttaccact aaaggcagag ttatcaccgg
    19681 ttaagtaagt cgtgccattg agcgcattga tatagcccag gccctcgctg ccactgacaa
    19741 tatccgcgga aatagtcgcg tttttatccg tgtggtttaa ggtgattttc ttatcggatg
    19801 tctttgccca cacaaactca attttttcgg catcaataat ccctgcagcc ttcgcagcac
    19861 ttccttcctg tgcacctaac gctaactcag agttggtgct taaactaatt ttcggtgcgg
    19921 atattttacc tccatctgat acgactaatg ttgtatcaga acgttggtcg taataaccca
    19981 ggctaatatg ttcacgagca ttaacttcac caccgttatt aaccgacagg aactgattac
    20041 cgccatatcc gccgtaaacg cccataaaga aggtccctgc atttacctga ctaccttcgc
    20101 cgcttacatt cccctgcgta taagtcagac gactggtgcc cttaacatga attttgccgc
    20161 cattggagac attaaaatag ccttgatcat gtctcatgcc tgagtttgac tcatacaaat
    20221 catcagcata taactcgctg ccagcattgg agacattcag gaaccccgta cctttcagca
    20281 aaaagttaag cgggccattg attgttacct ttcctccggt aatatccaac ccaccaccag
    20341 aagcgagagt cagacgcgga cgggaagtat ctgtcgtatt gattaccaga ttggcattgt
    20401 cagtcaccgt aatacggtaa tcgatgctct ggttctcttt gccaaaatca atagttttat
    20461 cctggtttac gatgacatca tcggcgaata ccagagatga atataaactt atcgcgctaa
    20521 ttaaaacagc ccgggacttt tttcctttta ctctgctccc cagctcagag cagacgctcc
    20581 aacagttacg ggacctgttc cactttagcc gatatatttt attcatcaca atctccagca
    20641 atacatccaa ataaaaccaa gattgcataa acagcaacgc attaaatgcc attctggtta
    20701 ctgttcatcg catat
//
LOCUS       DAZFRJ010000069        20544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000069 DAZFRJ010000000
VERSION     DAZFRJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.069"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(47..1405)
                     /gene="plaP"
                     /locus_tag="ACHKUN_002333"
     CDS             complement(47..1405)
                     /gene="plaP"
                     /locus_tag="ACHKUN_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO6660582.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1395..1457)
                     /gene="yoeI"
                     /locus_tag="ACHKUN_002334"
     CDS             complement(1395..1457)
                     /gene="yoeI"
                     /locus_tag="ACHKUN_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO6660583.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1672..2601)
                     /gene="yeeY"
                     /locus_tag="ACHKUN_002335"
     CDS             complement(1672..2601)
                     /gene="yeeY"
                     /locus_tag="ACHKUN_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6660584.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2647..3471)
                     /gene="yeeZ"
                     /locus_tag="ACHKUN_002336"
     CDS             complement(2647..3471)
                     /gene="yeeZ"
                     /locus_tag="ACHKUN_002336"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6660585.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3554..3808)
                     /gene="yoeB"
                     /locus_tag="ACHKUN_002337"
     CDS             complement(3554..3808)
                     /gene="yoeB"
                     /locus_tag="ACHKUN_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO6660586.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3805..4056)
                     /gene="yefM"
                     /locus_tag="ACHKUN_002338"
     CDS             complement(3805..4056)
                     /gene="yefM"
                     /locus_tag="ACHKUN_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO6660587.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4339..4386
                     /gene="hisL"
                     /locus_tag="ACHKUN_002339"
     CDS             4339..4386
                     /gene="hisL"
                     /locus_tag="ACHKUN_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO6660588.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    4362..4485
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4532..5431
                     /gene="hisG"
                     /locus_tag="ACHKUN_002340"
     CDS             4532..5431
                     /gene="hisG"
                     /locus_tag="ACHKUN_002340"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO6660589.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5437..6741
                     /gene="hisD"
                     /locus_tag="ACHKUN_002341"
     CDS             5437..6741
                     /gene="hisD"
                     /locus_tag="ACHKUN_002341"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO6660590.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6738..7808
                     /gene="hisC"
                     /locus_tag="ACHKUN_002342"
     CDS             6738..7808
                     /gene="hisC"
                     /locus_tag="ACHKUN_002342"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO6660591.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7808..8875
                     /gene="hisB"
                     /locus_tag="ACHKUN_002343"
     CDS             7808..8875
                     /gene="hisB"
                     /locus_tag="ACHKUN_002343"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO6660592.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8875..9465
                     /gene="hisH"
                     /locus_tag="ACHKUN_002344"
     CDS             8875..9465
                     /gene="hisH"
                     /locus_tag="ACHKUN_002344"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO6660593.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9465..10202
                     /gene="hisA"
                     /locus_tag="ACHKUN_002345"
     CDS             9465..10202
                     /gene="hisA"
                     /locus_tag="ACHKUN_002345"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO6660594.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10184..10960
                     /gene="hisF"
                     /locus_tag="ACHKUN_002346"
     CDS             10184..10960
                     /gene="hisF"
                     /locus_tag="ACHKUN_002346"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO6660595.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10954..11565
                     /gene="hisIE"
                     /locus_tag="ACHKUN_002347"
     CDS             10954..11565
                     /gene="hisIE"
                     /locus_tag="ACHKUN_002347"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO6660596.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11662..12639)
                     /gene="wzzB"
                     /locus_tag="ACHKUN_002348"
     CDS             complement(11662..12639)
                     /gene="wzzB"
                     /locus_tag="ACHKUN_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO6660597.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12786..13952)
                     /gene="ugd"
                     /locus_tag="ACHKUN_002349"
     CDS             complement(12786..13952)
                     /gene="ugd"
                     /locus_tag="ACHKUN_002349"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO6660598.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14201..15607)
                     /gene="gndA"
                     /locus_tag="ACHKUN_002350"
     CDS             complement(14201..15607)
                     /gene="gndA"
                     /locus_tag="ACHKUN_002350"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO6660599.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15718..16125)
                     /locus_tag="ACHKUN_002351"
     CDS             complement(15718..16125)
                     /locus_tag="ACHKUN_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO6660600.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(16116..17324)
                     /locus_tag="ACHKUN_002352"
     CDS             complement(16116..17324)
                     /locus_tag="ACHKUN_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6660601.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(17324..18454)
                     /gene="wecB"
                     /locus_tag="ACHKUN_002353"
     CDS             complement(17324..18454)
                     /gene="wecB"
                     /locus_tag="ACHKUN_002353"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO6660602.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(18451..19557)
                     /locus_tag="ACHKUN_002354"
     CDS             complement(18451..19557)
                     /locus_tag="ACHKUN_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFO6660603.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(19559..>20544)
                     /locus_tag="ACHKUN_002355"
     CDS             complement(19559..>20544)
                     /locus_tag="ACHKUN_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFO6660604.1"
                     /translation="NAVLKRFLDTDITEIRIFSRDEKKQDDMRKKYNNSKLKFYIGDV
                     RDYNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVLGTENVLEAAIANGIKRV
                     VCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVICGTRYGNVMASRGSVIPL
                     FVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIFVQKAPAATIQTLA
                     IALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDMGDYYRVPPDLRDLNYGK
                     YVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRALRSGEDYELDS"
BASE COUNT         5461 a         5087 c         4817 g         5179 t
ORIGIN      
        1 ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt
       61 cgtactgcgg aaccggatta cggaaactct tggttacgca agccaggtaa atcagaccga
      121 tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
      181 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct
      241 tgttacgctt ctcacggatc cagaactgcg aaataaccga caggttaacg aaggtgaacg
      301 ccaccagcgc accaaagtta atcagcgccg tagccattac caggtcgaag ttgattgcca
      361 gcaaggcaat cgcgccaacc aggatgatgt tcattgccgg agtacgccat ttcgggtgta
      421 cataaccgaa gaagcttttc gggaacacac cgtcacgccc cattacgtac atcagacgcg
      481 ctacgcctgc atgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
      541 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
      601 gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
      661 agatcatgcc gccaatcagt gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
      721 ctttggtttc ttccgacagg ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga
      781 ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgagtgc
      841 tcgccagcgt acccgcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca
      901 ccgcgatcag cactacctgc aacacgacga ttacagtgtt gaagttcgct acggatttca
      961 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
     1021 ggatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
     1081 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg
     1141 atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt
     1201 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
     1261 catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg
     1321 caagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
     1381 tgtttggagt aacgttatgc gacatggtca ttctccttta cggtgataac cgtcgcgtaa
     1441 gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa
     1501 atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat
     1561 aaccgacgct aattaaacgt cggtttttag tcattttttg acagcggcgc attgtgccta
     1621 aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc tttaaatggc
     1681 tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc
     1741 gtcccgccac caaccactgg caagccccgc ggtctgttca ttccagccaa tccagacggg
     1801 ttcaaagagg gtttgggcga ccactttctt ctcgattaac gcaccactat cgagaaaacg
     1861 ttgcgccaga taacggggta aatagccgca gccgaggccg ctaatttgca gctccagctt
     1921 ggttttaaaa tcgaaaacgg taatagcttc ttgttcgtcg agaagttgtg aggccgtaga
     1981 agcgccggcc tgtgcagtgt cccccaccac aatcgcccga tatcgtttaa tgatacgacg
     2041 gtttaacggt tcttcttcca gggctaacgg atgatgaggc gcgaccgcaa agacttgttc
     2101 cagatcgccc aaccgagaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc
     2161 aacaataata tccgctcgcc cctgagtcag ggcatcccat gaaccaccga gtacaccatt
     2221 gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcctcaatga gtggcgcaag
     2281 cagagaaaaa gggaaggtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg
     2341 cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc gccctttctc
     2401 taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctgcgat caagcaattg
     2461 aatattaagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta
     2521 gagtttggcc gacgccgccg caaaactgcc ttctttttct aaggcatcga ggatcatcaa
     2581 cacatccagc agtggtttca tacacgcccc cttgcagtgt atgcggcgtc ctgctggcgt
     2641 gatcgtttac tccagcggca ttaccagcgg atcgggatac tggtattcaa atcccagttc
     2701 attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa
     2761 ctgcggaggt tccagcccca ataaacgggc catctgcgga tagaaaacat tacgcgcagg
     2821 gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt
     2881 gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggggc
     2941 ggtttttccg gcaaagaagc gtccgggatg acgtcccggt ccgaccaggc ccgcaagacg
     3001 cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac
     3061 tcgtccactg ttggtcaccg gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc
     3121 gccatagaca gatgtcgagc tggtaaaaat aatgcgggga atacgatggg ccagcgcgct
     3181 atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc
     3241 cggaagcgta atgaccagcg catcggcatc catcagggca tccagatcgt cagaatcgca
     3301 aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc
     3361 gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat
     3421 cgccagcggc atgcctaacc accctaaccc gacaattgcg acctttttca tccgttatct
     3481 cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta
     3541 tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa tcatcagtaa
     3601 ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct gacaaattat
     3661 gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta tctttgataa
     3721 gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga taatcatccc
     3781 atgattcctc agaccagatt agtttcactc aataatgtcc ttttccgttc ctttgcctga
     3841 tttcaggcta tcgattgagt ccatcaatct ccgggcgtta gcgggggaac gcagtagata
     3901 agccgtctct tccagcgagt tgtattcttc gagtgacatc agaacacaag cctctccatt
     3961 ctgacgagta atgaggatcg gggcatgatc ttcaacggct ttcatcattg ttgccgacaa
     4021 attctgacgc gcttcgctgt agctaattgt acgcatgtaa atctcctctt ttgtacagtt
     4081 cattgtacaa tgatgggtgt taattcacta tttattaatt agtttgtaga tcaagacatt
     4141 gccagcgaga cgaaaaatca gactttgctc tttttggtgc tgtcaagtta gaggacagtc
     4201 ctcttagccc cctccttttc ccgctcattc attaaacaaa tccatcgcca taaaatatat
     4261 aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt aaaagacatc agttgaataa
     4321 acattcacag agacttttat gacacgcgtt caatttaaac accatcatca ccatcatcct
     4381 gactagtctt tcaggcgatg tgtgctggaa gacattcaga tcttccagtg gtgcatgaac
     4441 gcatgagaaa gcccccggaa gatcaccttc cgggggcttt tttattgcgc ggttgttaac
     4501 ggttcagaca ggtttaaaga ggaataagaa aatgacagac aacactcgtt tacgcatagc
     4561 tatgcagaaa tccggccgtt taagtgatga ctcacgcgaa ttactggcgc gctgtggcat
     4621 taaaatcaat cttcacaccc agcgcctgat cgcgatggca gaaaacatgc cgattgatat
     4681 tctgcgcgtg cgtgacgatg acattcccgg tctggtaatg gacggcgtgg tagaccttgg
     4741 gattatcggc gaaaacgtgc tggaagaaga gctgcttaac cgccgcgccc agggtgaaga
     4801 tccgcgctac tttaccttgc gtcgtctgga tttcggcggc tgccgccttt cgctggcaac
     4861 gccggttgat gaagcctggg acggtccgct ctccttaaat ggtaaacgta tcgccacctc
     4921 ttatcctcac ctgctcaagc gttatctcga ccagaaaggt atctctttta aatcctgctt
     4981 actgaacggc tctgtcgaag ttgcgccgcg tgccggactg gcggatgcga tttgcgatct
     5041 ggtttccacc ggtgccacgc tggaagctaa cggcctgcgc gaagtcgaag ttatctaccg
     5101 ctcgaaagcc tgcctgatcc agcgcgatgg cgaaatggaa gaatccaaac agcaactgat
     5161 cgacaaactg ctgacccgta ttcagggtgt gatccaggcg cgcgaatcaa aatacatcat
     5221 gatgcacgca ccgaccgaac gtctggatga agtcatcgcc ctgctgccag gtgccgaacg
     5281 cccaaccatt ctgccgctgg cgggtgacca acaacgcgta gcgatgcaca tggtcagtag
     5341 cgaaaccctg ttctgggaaa ccatggaaaa actgaaagcg ctgggtgcca gttcaatcct
     5401 ggtcctgccg attgagaaga tgatggagtg atcgccatga gctttaacac aatcattgac
     5461 tggaatagct gtactgcgga gcaacaacgc cagctgttaa tgcgcccggc gatctccgct
     5521 tctgaaagca ttacccgcac cgttaacgat attctcgata acgtgaaagc acgcggcgat
     5581 gacgccctgc gggaatacag cgcgaagttt gataaaacca cggttaccgc gctgaaggtg
     5641 tctgctgagg agatcgccgc cgccagcgaa cgccttagcg acgagctaaa acaggcgatg
     5701 gcggtggcag taaaaaatat tgaaaccttc cacactgcgc aaaaactgcc gccggtagat
     5761 gttgaaacac agccaggcgt gcgttgccag caagtcacgc gcccggtagc ttcagttggg
     5821 ttgtatattc ctggcggctc cgccccactc ttctcaacgg tattaatgct ggcaactcct
     5881 gcgcgtattg cgggctgtaa aaaagtggtg ttgtgctcac cgccgccgat tgccgatgag
     5941 atcctttatg cggcgcagct gtgcggtgtg caggacgtgt ttaacgtcgg cggcgcacag
     6001 gccattgccg ctctggcgtt tggtacggaa tctgtgccga aagtggacaa aatcttcggg
     6061 ccgggtaacg cctttgtcac cgaagcgaaa cgtcaggtga gccagcgtca ggacggtgcg
     6121 gcgatcgata tgcccgcagg cccgtcggaa gtgctggtga ttgctgacag cggcgctacg
     6181 ccagatttcg tggcttctga tttgctttct caggctgaac acggcccgga ctcacaggtg
     6241 attttactga cgcccgctgc tgatatggcg cgtcgcgttg ccgaggccgt cgaacgccag
     6301 ttggcagaac tgccgcgtgc cgaaaccgcc cgccaggcac tgaacgccag ccgcctaatc
     6361 gtgaccaacg atttagcaca gtgcgtggag atctccaacc agtacggccc ggagcacctg
     6421 atcattcaga cccgcaacgc tcgcgatctg gtcgatagca tcaccagcgc cggttcggta
     6481 tttcttggtg actggtcacc ggaatcggca ggtgattacg cctccggcac caaccacgtt
     6541 ctgccgactt acggttacac cgccacctgt tccagcctcg ggctggcaga tttccagaag
     6601 cgcatgaccg tgcaggaact gtcgaaagaa ggcttttctg cgctggcttc aaccatagaa
     6661 acactggccg ccgccgagcg cctgaccgcc cataaaaatg ccgttacttt gcgtgttaac
     6721 gcccttaagg agcaagcatg agcaccgtga ctattaccga tttagcgcgt gaaaacgtcc
     6781 gcaacctgac gccgtatcag tcggcgcgtc gtctgggcgg taacggcgac gtctggctga
     6841 acgccaacga atatcccgca gccgtggagt ttcagcttac tcaacaaacg ctcaaccgct
     6901 acccggaatg ccagccgaaa gcggtgattg aaaattacgc gcaatatgca ggcgtaaaac
     6961 cggagcaggt gctggtcagc cgtggcgcgg acgaaggtat tgaactgctg attcgcgctt
     7021 tttgcgaacc gggtaaagac gccatcctct actgcccgcc aacgtacggc atgtacagcg
     7081 tcagcgccga aacgattggc gtcgagtgtc gcacagtgcc gacgcaggac aactggcaac
     7141 tggacttaca gggcatttcc gacaagctgg acggcgtaaa agtggtctat gtttgcagcc
     7201 ccaacaaccc gaccgggcaa ctgatcaatc cgcaggattt tcgcactctg ctggagctaa
     7261 cccgcggaaa agccattgtg gttgccgatg aagcctatat agagttttgt ccacaggcat
     7321 cgctggctgg ctggctggcg gaatatccgc acctggctat tttgcgcaca ctatcgaaag
     7381 cttttgctct ggcggggctt cgttgcggat ttacgctggc aaacgaagaa gtcatcaacc
     7441 tgctgatgaa agtgatcgcc ccctacccgc tctcgacgcc ggttgccgac attgcggccc
     7501 aggcgttaag cccgcagggg atcgtcgcca tgcgcgaacg ggtggcgcaa attattgcag
     7561 aacgcgaata cctgattgcc gcactgaaag agatcccctg cgtggagcag gtttttgact
     7621 ctgaaaccaa ctacattctg gcgcgcttta aagcctccag tgcagtgttt aaatctttgt
     7681 gggatcaggg cattatctta cgtgatcaga ataaacaacc ctctttaagc ggctgcctgc
     7741 gaattaccgt cggaacccgt gaagaaagcc agcgcgtcat tgacgcctta cgtgcggagc
     7801 aagtttaatg agtcagaagt atctttttat cgatcgcgat ggaaccctga ttagcgaacc
     7861 gccgagtgat tttcaggtgg accgttttga caaactcgcc tttgaaccgg gcgtgatccc
     7921 ggagctgctg aagttgcaaa aagcgggcta caaactggtg atgatcacta atcaggatgg
     7981 tctgggaaca caaagtttcc cgcaggcgga tttcgatggc ccgcacaacc tgatgatgca
     8041 gatcttcacc tcgcaaggcg tacagtttga tgaagtgctg atttgtccgc acctgcccgc
     8101 cgatgagtgc gactgccgta agccgaaagt aaaactggtg gagcgttatc tcgctgagca
     8161 agcgatggat cgtgccaata gttatgtgat tggcgatcgc gcgaccgaca ttcaactggc
     8221 ggaaaacatg ggtattaatg gtttacgcta cgaccgcgaa accctgaact ggccaatgat
     8281 tggcgagcaa ctcactaaac gagaccgtta cgcccatgta gtgcgtaaca ccaaagagac
     8341 gcaaattgac gtccaggtgt ggctggatcg cgaaggtgac agcaagatta acaccggcgt
     8401 tggcttcttt gatcacatgc tggatcagat cgccacccac ggcggtttcc gtatggaaat
     8461 caacgtcaaa ggcgacctct atatcgacga tcaccacaca gtcgaagata ccggcctggc
     8521 gctgggtgaa gcgttaaaaa ttgcccttgg cgacaaacgc ggtatttgcc gctttggttt
     8581 tgtgctaccg atggacgaat gccttgcccg ctgcgcgctg gatatctctg gtcgcccgca
     8641 cctggaatat aaagccgagt ttacctacca gcgcgtgggc gatctcagca ccgaaatgat
     8701 cgagcacttc ttccgttcgc tctcttacac catgggcgtg acgctacacc tgaaaaccaa
     8761 aggtaaaaac gatcatcacc gtgtagagag cctgttcaaa gcctttggtc gcaccctgcg
     8821 ccaggccatc cgcgtggaag gcgacaccct gccctcgtca aaaggagtgc tgtaatgaac
     8881 gtggtgatcc ttgataccgg ctgcgccaat ctgaactcgg tgaagtctgc cattgcgcgc
     8941 cacggttatg aacccaaagt cagccgtgac ccggacgtcg tgttgctggc cgataaactg
     9001 tttttacccg gcgtaggcac cgcacaagca gcgatggatc aggtgcgtga gcgcgaactg
     9061 tttgatctca tcaaagcctg tacccaaccg gtgctgggaa tctgcttagg gatgcaactg
     9121 ctggggcggc gcagcgaaga gagcaacggc gtcgacttgc tgggcatcat tgacgaagac
     9181 gtgccgaaaa tgaccgactt tgctctgcca ctgccacata tgggctggaa ccgcgtttat
     9241 ccgcaggcag gcaaccgcct gtttcaggga attgaagacg gcgcgtactt ttactttgtt
     9301 cacagttacg caatgccggt caatccgtgg accatcgccc agtgtaatta cggcgaaccg
     9361 ttcaccgcgg cggtacaaaa agataacttc tacggcgtgc agttccaccc ggagcgttct
     9421 ggtgccgctg gcgctaagtt gctgaaaaac ttcctggaga tgtgatgatt attccggcat
     9481 tagatttaat cgacggcact gtggtgcgtc tccatcaggg cgattacggc aaacagcgcg
     9541 attatggtaa cgacccgctg ccgcgattgc aggattacgc cgcgcagggt gcagaattgt
     9601 tgcatctggt ggatctgacc ggggcaaaag atccggctaa acgtcaaatc ccgctgatta
     9661 aaaccctggt ggcgggcgtt aacgtgccgg tgcaggttgg cggtggcgtg cgtagcgaag
     9721 aagacgtggc ggcgttactg gaagcaggcg ttgcgcgcgt ggtggttggc tccaccgcgg
     9781 tgaaatcacc agagatggtg aaaggctggt ttgaacgttt cggtgccgat gccttagtgc
     9841 tggcgctgga tgtccgtatt gacgagcaag gcaacaagca ggtggcagtc agcggctggc
     9901 aagagaactc gggcgtttca ctggaacaac tggtggaaag ctatctgccc gtcggcctga
     9961 aacatgtgct gtgtaccgat atctcgcgcg acggcacgct ggcaggttct aacgtctctt
    10021 tatatgaaga agtgtgcgcc agatatccgc aggtggcgtt tcagtcctcc ggcggtattg
    10081 gcgacattga tgatgtggcg gccctgcgtg gtactggcgt gcgcggcgta atagttggtc
    10141 gtgcattact ggaaggtaaa ttcaccgtga aggaggccat cgcatgctgg caaaacgcat
    10201 aataccgtgt ctcgacgtcc gtgatggtca ggtggtgaaa ggcgtacagt ttcgcaacca
    10261 tgaaatcatt ggcgatatcg tgccgctggc aaaacgctac gctgaagaag gtgcagacga
    10321 actggtgttc tacgatatca ccgcttccag cgatggccgt gtggtagata aaagctgggt
    10381 atctcgcgtg gcggaagtga tcgacattcc gttctgcgtg gcgggtggga ttaagtctct
    10441 ggaagatgcc gcgaaaattc tttcctttgg cgcggataaa atttccatca actcccctgc
    10501 gctggcagac ccaacattaa ttactcgcct ggcggatcgt tttggcgtgc agtgtattgt
    10561 ggtgggtatt gatacctggt acgacgctga aaccggtaaa tatcatgtga atcaatatac
    10621 cggcgatgaa agccgcaccc gcgtcactca gtgggaaaca ctcgactggg tacaggaagt
    10681 gcaaaaacgc ggtgccggag aaatcgtcct caacatgatg aatcaggacg gcgtgcgtaa
    10741 cggttacgac ctcgagcagc tgaaaaaagt gcgagaagtt tgccgcgtcc cgctgattgc
    10801 ctccggtggc gcgggcacca tggaacactt cctcgaagcc ttccgtgatg ccgacgttga
    10861 cggcgcgctg gcggcttccg tattccacaa acaaataatc aatattggtg aattaaaagc
    10921 gtacctggca acacagggcg tggagatcag gatatgttaa cagaacaaca acgtcgcgaa
    10981 ctggactggg aaaaaaccga cggactgatg ccggtgattg tgcaacacgc ggtatctggc
    11041 gaagtgctaa tgctgggcta tatgaacccg gaagccttag acaaaaccat cgaaagcggc
    11101 aaagtcactt tcttctcgcg cactaaacag cgactgtgga ccaaaggcga aacgtcgggc
    11161 aatttcctca acgtagtaaa tattgcgccg gactgcgaca acgacacgct actggtactg
    11221 gcgaatccca tcgggccaac ctgccacaaa ggcaccagca gctgcttcgg cgacaccgct
    11281 caccagtggc tgttcctgta tcaactggaa caattgctcg ccgaacgcaa atctgccgat
    11341 ccggaaacct cttacacagc caaactgtat gccagcggca ccaaacgcat tgcacagaaa
    11401 gtgggcgaag agggcgtgga aaccgcacta gcagcaacgg tacatgaccg ctttgaactc
    11461 accaacgagg cgtctgactt gatgtatcac ctgttggtgt tgttgcagga tcaggatctg
    11521 gatttaacaa cggtgattga gaacctgcgt aaacggcatc agtgagttgc ggggtaagcg
    11581 gatgcgatgt tgttgtcgca tccggcaaaa aaaacgggca aggtgtcacc accctgcccg
    11641 ttttctttaa aaccaaaaag attacttcgc gttgtaatta cgcagagcgt tacgccccag
    11701 cacaatcccc gcgccaacca tgccgcccag cagcaccgcc agaatcaaag tgattgcctt
    11761 tttcgggcta tcgcgacgaa taggtaacgt cggtttcatc acatagcggt aagcatgaat
    11821 atcaagatca tcaactttta agctttcgat atccagcagg ttttgacgtg tctgatagta
    11881 gtttggtgag aacaccaacg gacgagtcgc ttcatgctta atcatcgact ccagcgcttc
    11941 gctccccaaa aggaacaacg tatcttgtgt cacatcttca gtctgctgaa cctgtggctt
    12001 tgtcacctgc gcctgattcg catactgcaa cgcttcctga atctgacgga tacgcagatc
    12061 tttctgctcc tgcgcaacta cttcctgagt tttcagagaa tcctgcaagt ttttcatacg
    12121 cagcgcgatg ttgtctttca ggtcttttcc cagttcttca tttacctggt cgtcaacttg
    12181 ctggatatac ttcgccagct gcttctgagc accttcaggt gtttcaccaa cataagacac
    12241 cgctaaaggt aatgactgat ttttgacggt aggctcaatg gtcagctttt ctgcttcttc
    12301 ctggttatcc agcgtttccg ctaatgctga gaatgcagtg ctgtaacgtc cgataagtga
    12361 agcctgaatg tcggagactt tcggtacagc cgatccgtag atcacattca tcgcgttggt
    12421 ataaccagcg atttgcccca catcaggttg agtaacgata gctgtggaag tccacttttc
    12481 tttggcaacg gccagatacc ctatagccag ggcaatagca acaatgacag agatgataat
    12541 tgtcatcttg ccacgccaca actgcaccag taaatcaatc aaatcaatct gttccgggtc
    12601 atggttttgc ccagaaacat tattattttc tactctcatc attaccctaa cggacgaaat
    12661 aattttaagt cgccatagtt tagcgataat cgccatgaat gctataggat aaatgataaa
    12721 aataatgaat tattacaaag ggaacataag agaatcaggg cgaaaatcgc cctgataaca
    12781 ggatgttagt cactgccaaa gagatcgcgg gtgtagactt tgtctgccac atccttaagc
    12841 tcttctgcca tacggttgga gataattacg tcggcttgtt gtttgaaagt ggcgagatca
    12901 cgttccagcc gagagttgaa gaatgagtct tctttcatca ctggctcgta gatgattact
    12961 tcaacacctt tcgccttgat acgcttcata atcccctgaa tagaagacgc acggaagtta
    13021 tctgaaccgc tcttcataat caggcgataa atacccacca catgcggttt gcgtgacaaa
    13081 atggcatcgg caataaaatc tttacgcgtg cggtttgcat cgacaattgc cgagatcagg
    13141 ttattcggca cagactgata gttcgccagt aactgcttgg tatctttcgg cagacaataa
    13201 ccaccataac caaacgacgg attgttgtaa tggttgccaa tacgcggatc gagacaaacg
    13261 ccttcgataa tctggcgagt attcagacct aaactttctg cgtagctatc cagttcatta
    13321 aagtacgcta cgcgcatcgc cagataggta ttagcgaaaa gtttaatcgc ttctgcttcg
    13381 gttgagtcgg taaacagggt cgggatattt tgcttaatcg cgccttcctg taacagagca
    13441 gcaaaacgtt ctgcgcgttc tgaacgctca ccgatgacaa tacgtgaagg atggagatta
    13501 tcgtaaaggg ctttaccctc acggagaaat tccggagaga aaataatatt tttagtacga
    13561 tatttcttat gcatcgctgc ggtaaagcca acgggtaccg ttgatttgat gaccataacc
    13621 gcataaggat ttatctctac tacgtcctta attactgatt ctacactcga tgtattgaaa
    13681 taattagtgt taggatcata gtcggttgga gtggcgatga tgacataatc agcatcccgg
    13741 taggcttcat ttttatctaa tgtggcatta aagtgtattt tatctgattg caaaaactgc
    13801 tgaatttcct tatcaacaat aggagatatc cgatcattca gcatagcaac gcgtgacggt
    13861 aaaatatcta atgccacaac ctcatgattt tgtgcgatta gaagcccgtt tgacaagcct
    13921 acatagccgg taccggaaat ggtgattttc atttcatgct ctcagaatta acttaactgt
    13981 gaatcatgat gtttttagca tcctgataag agctaaaagt tttaacgcta cgttgttcga
    14041 tggtcaacgc aacgaaaaac aattcagatt ttctttataa gaatagctga tatttattaa
    14101 gttaatatta agcagtgaaa tttagtctat aagataattg gcaaaaaaaa agcccggcgt
    14161 aatatacgcc gggcctcaat ttattgttgg ttaaatcaga ttaatccagc cattcagtat
    14221 ggaacacacc ttctttatca atgcgcttat aagtatgcgc accaaaatag tcacgctgag
    14281 cctggatcag gttcgcaggc agaacagcgg cacggtagct gtcataatag gcaaccgcag
    14341 cggcgaaggt cggaaccggg ataccgttct gtactgcata agcaacgaca tcacgcagcg
    14401 cctgctggta gtcatcggca atttgcttga agtacggagc caacagcagg ttagcgatct
    14461 gaggattttc ggcataagca tcagttattt tctgcaggaa ctgcgcacgg atgatgcagc
    14521 cagcacggaa aatcttcgcg atttcgccgt agttcagatc ccagttgtac tcttcagacg
    14581 ctgcgcgcag ctgagagaag ccctgagcgt aagaaacgat tttgcccaga tacagcgcac
    14641 ggcgaacttt ttcgatgaac tcagccttgt cgccagctgg ctgcgcttgc gggccagaga
    14701 gaactttaga tgcggcaaca cgctgatctt tcagagaaga gatataacgt gcaaacacag
    14761 actcggtaat cagcgacagc ggttcaccga gatccagcgc gctctggctg gtccatttac
    14821 cggtaccttt gttagccgct tcatccagga tcacatcaac caggtagtta ccgtcttcat
    14881 cttttttggt gaagatgtct ttggtgatgt cgatcaggta gctactcagt tcaccgttat
    14941 tccactcggt aaaggtctgt gccagttctt cgttagagag attcaggccg cctttcagga
    15001 gagaataggc ttcagcaatc agttgcatat caccgtattc aataccgttg tgaaccatct
    15061 tcacatagtg acccgcgcca tcagcaccaa tataggtaac gcacggttcg ccatcttcag
    15121 caacggcggc gattttagtc aggatcggtg caaccagttc ataggcttct ttctgcccac
    15181 caggcataat ggaaggacct ttcagtgcgc cttcttcacc accggagaca ccggtaccaa
    15241 tgaagttaaa accttctgca gaaagctcac gattacgacg aatggtgtcc tggaagaagg
    15301 tattaccacc atcaatgatg atgtcacctt tatcgaggta tggcttaagg gaatcaatag
    15361 cagcatccgt gcctgcacct gctttcacca ttaacaggat gcgacgaggc gtttccagag
    15421 attcaacaaa ctctttcacc gtatagtaag gaaccagttt cttgcctgga ttttcggcaa
    15481 tcacttcttc cgtcttttca cgggaacggt tgaaaataga gacggtataa ccacggcttt
    15541 cgatgttgag cgcaaggttg cgccccatca ctgccatacc gactacgccg atctgttgct
    15601 ttgacattgt ttactcctgt caggggtggt cacgcggggt tacgcggctt gaagaagcta
    15661 agatggtaac ccagcatgta tagaataaga tagaagaata aacggtggtg tatcgcccta
    15721 taaccactta gaaaaagcct tagctttcag tgggtcaaat ggcccctcct ttatctctaa
    15781 cataagggct tttggtgaca ggcatttgac actatgtata tctcctgggg aaaattccac
    15841 gacacttatt cccgtaccgt ctccaacaac aaactttttt tggagctcac cattttgctc
    15901 atacaaacaa acttctaatt gcccttgcat gacgacaaac atttcccact gatgcggtaa
    15961 ctcatgccaa tggggttcaa catagctgtc gcgtacaaat gcaataagta aacgttgaac
    16021 cttctcctga tgagatgcgt gcaataaata atgagcgcgc aaacgttcag aatttgctgc
    16081 agtgcgaaaa agttcatcca taatattgtc atcaactaaa cgcattctcc tgcctccagt
    16141 cggacttcga tagtatgtgc cgccgattca accgaaaatt gagactttaa caacacattg
    16201 gcgttctgac ctaactgttt tcttaaaact gaatcactaa gaagcaattg tgcagattca
    16261 aacagtttat catcttcacc attaacatga atgaacccag ccctgtgctt attaattaca
    16321 tccattagat cattaccgcc attcacactc ccaaggatcg ggattgattg aaccatatac
    16381 cctagtaatt ttccggggaa attatgtgaa gaatggcgag acgaaaggga gaacaggcca
    16441 acatcaactt cagataaaat taatttaaac tcttcctggt tcactgaagg tagatgagtg
    16501 aaattagtta aattccattc tgcagaaaga gatttaatca ggtcaacttc atcaccctgc
    16561 cctataaaca ggaaatgagc atcatgatga cgcatcatat tacgcgcaag gcgcattaag
    16621 tttgccatat cctgagcatg cccaatatta ccgccataga aaaaaatgat tttatctttt
    16681 agagcgtatt tttgacgaag tgaatgataa tcatcgccgg cagacacagg agtcattgag
    16741 gcccaattac gtaaaacttc acacggataa cctttattgg cctgacgaaa tatctcaaga
    16801 ttcttatcag acattaaccc aatccagtca gcctgttgat atgatttttt ttcaaaatac
    16861 ctgaaatatt tttcaattgg cgagccggct tttatcatac cagcatcaat gacccactgt
    16921 gggaacatat cccttaggac cagatagctt gggcactggc atcgctcttt aattttttta
    16981 accaagcctc cccaaaaaat agagggggaa taataaacga taccatcaaa tgtatcatgt
    17041 tggatgagat gctttaatgc gtgccatgca cgaaaagata aaagagtttc atttatggca
    17101 cgtttagcct tacctatatc ctttaaaggt ccacttttga aacgccaaac ctttatacca
    17161 tcagtcatgc taacagaata gattgcctgt aatgtgttgt caggcgtaat tacagttaca
    17221 tcatggcctc tgctcagcaa ttccaggcct aactcatgaa acattttagc cccaactcgt
    17281 gtgctatggg gcaaataatc atcaatgatt aatgcaagtt tcattagtat tgcttccaga
    17341 caacccgttt aacgtagtca gtatatgaat ggataatacg cacaacttta tctgaaacat
    17401 ttggcatact atagtcactg acctgacgaa gtaagcgtac ttctccacga ggctgtgttg
    17461 caataatttc taatgcctgt aacacacggg cagactcaag accaaccatc attacagccc
    17521 cttcttcgaa gccttcaggg cgttcgtgcg cttctcgtat attgagcgca gggaagttca
    17581 taatggagga ctcctctgta atagtcccgc tatccgataa aacagcacgt gcattttttt
    17641 gcagatggtt gtaatcgtga aatcctaatg gcttaagtag caagatattt ttatgaaatt
    17701 gaataccgtt ttcgttgatg cggttacgag tgcgcggatg agtagaaaca actaccggga
    17761 tatcatattt ttcagccacg gtattaagta tatttgccag tttcgcaagc tgtttagggg
    17821 tatcaacatt ttcttctctg tgggcactta ccacaaagaa attcccaggt gttaaattca
    17881 ggcgagaaag tacatcggaa ccatcaatct gcgacatata atgagtgagt acttcaaaca
    17941 ttgggctacc ggttttaata atcctatcgg ctggtacacc ttcagccaaa agatattcac
    18001 gcgcgatatc actatatgtc atattgatat cagcggtgtg gtcaactatt tttctgttag
    18061 tttcttccgg tacgcgttga tcaaaacaac gattcccagc ctccatatgg aaaatcggaa
    18121 ttcttcgacg ctttgctggt attgctgaaa tacaggagtt agtatcgcca agaactaaca
    18181 tggcttctgg tttttcctgt tcaaagacct catcaacttt aatgataact tgtccaatag
    18241 tctctgctgc atttttacct gcggcattaa gaaaataatc aggtttgcga acacccaaat
    18301 ccttgaaaaa aacctcattc aattcataat cgtaattttg cccagtatga acaataaggt
    18361 gatcacaata ttcatctaat tttgcaagga cacgcgagag tcgaataatt tctggacgag
    18421 tcccaacaac cgacatgact ttcaattttt tcacgataaa actctcgcta tagtatctgg
    18481 ttcagaacga ttaaatattt catttgccca aagcataaca actagctcat ccgagccatt
    18541 attagtaatg tcatgcgtcc atcccggaac tgtttcaaca attttaaaat catctgatga
    18601 aacattaagt tcatatcgtt cacccgtgac aatattttca aatttgaaac aagcacttcc
    18661 tcggatgata ataaatttct catttttgga atgatgataa tgcccccccc gagtaattcc
    18721 tggatgcgca gtaaaaaacg aaaactggcc cgcgtttttc gttttcaata cttcacagaa
    18781 tacccctctg tcatcactat aagaaggaac cgtatacgca aactgtttag gagaaaggta
    18841 acttaaccat gttgagtaca atgctcgagt gaatccatta cctacatctt cgatgattaa
    18901 tgtttggcga ctttctttaa atcgataaat taattgtgcc acctcaccaa cagtaacaga
    18961 ataaattgga ccaaatgtcc tgtaaccagt ttcgttcgct ccttctaata gctttaatat
    19021 gtcagaacaa aagtcatcta tatacaccaa atttacaact gctgaaggat cattaattgt
    19081 aatagcttca tcatttgcaa tgcgatggca gaaagttgct ataaaggagt tataatttgg
    19141 ttgacaccac ttaccgaata cattcggcaa gcgataaata taatatttag cgtttgtcgt
    19201 ttccccatac tgctgaatga ttttttccgc aactgcttta ctctttccat aagcgttatc
    19261 acattcagct tggatggatg aactaagcat gataggagta tctttattat tttttttcag
    19321 aatatcaaca atctgttgag ttagatcgca attcccctct tcaaactcac tctccttcac
    19381 aggacgattt actccagcaa gatgaaaaat gaaatctgcc tgcttaagtc cctgctctaa
    19441 atccgtcaaa gaagagttac gatctatcgc aataagttcg ttatatccag cttccttaag
    19501 gcggaaaacc aaatttcgac cgataaaccc tgcagcgcca gtaactaaaa ttttcatatt
    19561 atgaatccaa ctcataatct tcaccagaac gaagtgcccg gataaaagga agttttagca
    19621 gtaattcttt cattccctca acatctaatc tctcagtatt atgagaatta taatcttcca
    19681 cttccgagat acgacggtca ccatgttcca catattttcc atagttcaaa tcgcggagat
    19741 ctggtggaac acgataataa tcacccatat cttccgctgc aatcatttcc tctcggctca
    19801 ataacgcttc gtaaagtttt tccccgtgtc gagttccaat aatattgatt ggatgctcat
    19861 gggcatttag caattcctta agtgcaatgg ctaatgtttg aattgttgcc gcaggtgctt
    19921 tctgaacgaa aatgtcacca ttatttccat gttcgaaagc ataaaggacc agatcgacag
    19981 catcctcaag cgtcatcatg aaacgagtca tattgggatc ggttatggtc aatggtttac
    20041 cagctttgat tagatcaaca aataatggga tgaccgatcc acgtgaagcc attacatttc
    20101 cataacgagt tccacagata actgtttttg aactgtcaag attacgtgat tttgcaacaa
    20161 taactttttc catcattgcc ttagatatgc ccattgcatt gataggataa acggctttat
    20221 cggtactcaa gcacaccacg cgtttaatcc cattcgcaat agcagcctcc agaacatttt
    20281 ccgtacccag aacatttgtc ttaaccgcct ccatagggtg aaattcacat gaaggaactt
    20341 gtttaagggc tgctgcatga tacagaaaat cggcaccacg cgttgcattt agaacggaat
    20401 tatagtctcg cacatcacct atataaaatt ttaattttga gttattatat tttttccgca
    20461 tatcatcttg ttttttttca tccctgctaa atattcgtat ttcagtaata tctgtatcta
    20521 gaaaacgctt aagtacggca ttac
//
LOCUS       DAZFRJ010000070        20403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000070 DAZFRJ010000000
VERSION     DAZFRJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.070"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(495..1691)
                     /gene="hemY"
                     /locus_tag="ACHKUN_002356"
     CDS             complement(495..1691)
                     /gene="hemY"
                     /locus_tag="ACHKUN_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO6660605.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1694..2899)
                     /gene="hemX"
                     /locus_tag="ACHKUN_002357"
     CDS             complement(1694..2899)
                     /gene="hemX"
                     /locus_tag="ACHKUN_002357"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO6660606.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2921..3661)
                     /gene="hemD"
                     /locus_tag="ACHKUN_002358"
     CDS             complement(2921..3661)
                     /gene="hemD"
                     /locus_tag="ACHKUN_002358"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO6660607.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3658..4599)
                     /gene="hemC"
                     /locus_tag="ACHKUN_002359"
     CDS             complement(3658..4599)
                     /gene="hemC"
                     /locus_tag="ACHKUN_002359"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO6660608.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4986..7532
                     /gene="cyaA"
                     /locus_tag="ACHKUN_002360"
     CDS             4986..7532
                     /gene="cyaA"
                     /locus_tag="ACHKUN_002360"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO6660609.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(7572..7892)
                     /gene="cyaY"
                     /locus_tag="ACHKUN_002361"
     CDS             complement(7572..7892)
                     /gene="cyaY"
                     /locus_tag="ACHKUN_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO6660610.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7940..9265)
                     /locus_tag="ACHKUN_002362"
     CDS             complement(7940..9265)
                     /locus_tag="ACHKUN_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO6660611.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9262..10770)
                     /locus_tag="ACHKUN_002363"
     CDS             complement(9262..10770)
                     /locus_tag="ACHKUN_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO6660612.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11108..11662)
                     /locus_tag="ACHKUN_002364"
     CDS             complement(11108..11662)
                     /locus_tag="ACHKUN_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO6660613.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            12012..12215
                     /gene="yifL"
                     /locus_tag="ACHKUN_002365"
     CDS             12012..12215
                     /gene="yifL"
                     /locus_tag="ACHKUN_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6660614.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12252..13076
                     /gene="dapF"
                     /locus_tag="ACHKUN_002366"
     CDS             12252..13076
                     /gene="dapF"
                     /locus_tag="ACHKUN_002366"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO6660615.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            13073..13780
                     /gene="yigA"
                     /locus_tag="ACHKUN_002367"
     CDS             13073..13780
                     /gene="yigA"
                     /locus_tag="ACHKUN_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO6660616.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13777..14673
                     /gene="xerC"
                     /locus_tag="ACHKUN_002368"
     CDS             13777..14673
                     /gene="xerC"
                     /locus_tag="ACHKUN_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO6660617.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14673..15389
                     /gene="yigB"
                     /locus_tag="ACHKUN_002369"
     CDS             14673..15389
                     /gene="yigB"
                     /locus_tag="ACHKUN_002369"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO6660618.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15473..17635
                     /gene="uvrD"
                     /locus_tag="ACHKUN_002370"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15473..17635
                     /gene="uvrD"
                     /locus_tag="ACHKUN_002370"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO6660619.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17782..18546)
                     /gene="yigE"
                     /locus_tag="ACHKUN_002371"
     CDS             complement(17782..18546)
                     /gene="yigE"
                     /locus_tag="ACHKUN_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO6660620.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18708..18764
                     /gene="ysgD"
                     /locus_tag="ACHKUN_002372"
     CDS             18708..18764
                     /gene="ysgD"
                     /locus_tag="ACHKUN_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO6660621.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18916..19866
                     /gene="corA"
                     /locus_tag="ACHKUN_002373"
     CDS             18916..19866
                     /gene="corA"
                     /locus_tag="ACHKUN_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO6660622.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19908..20400)
                     /locus_tag="ACHKUN_002374"
                     /pseudo
     CDS             complement(19908..20400)
                     /locus_tag="ACHKUN_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
BASE COUNT         4785 a         5240 c         5280 g         5098 t
ORIGIN      
        1 gtaaggcagg tttattatat cgcgttgatt attgatgctt tttttagttt taacggcaat
       61 taatatatgt gttattaatt gaatgaattt tatcattcat aataagtatg tgtaggatca
      121 agctcaggtt aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
      181 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacgg
      241 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      301 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      361 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      421 ctccgtaata agagcaggcg tttttttatg catcagaaag gtcccggagg tgcttgcctc
      481 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      541 cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      601 taatcgtagg cgtccggacg ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc
      661 cattctccgt gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg
      721 acgtttttga tctgctggcg cagcactttt tccagctgtt ccggattgtt ggttttcagt
      781 cgaggaatcg gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt
      841 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      901 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta
      961 ccgttatcgg cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
     1021 cgatgttctt catcaccaac atgggctttc gccattgatg ggatgatatc cagcagcgaa
     1081 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1141 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1201 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctctgct
     1261 gcgcgttcca gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca
     1321 gccagtagat agttcaccac cggttgttcc gcgtgatcgg catttttcgc catcagcttt
     1381 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgg
     1441 gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt gtgcgcctgt gcggaagatc
     1501 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcc
     1561 aggcccgtga cgctggtttc gatattgtag ttgtcagtct ggatcagcac gtaaccctga
     1621 tgaccggcaa tcatcgggcc aaccactatc cccgcaatca gcaaaacaaa gagtaataac
     1681 acttttagca tggttattct ccttgcggcg cggctgccgg agtatcacct tgcggtgcag
     1741 gtgcaggtgc aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt
     1801 gtgccagcag gttacgcacg cgggtctgca tcagtttttc cagcatcgcc tggctttgca
     1861 gggtttccgg aagatccatc gagatatttt gctggcttaa ctggtccacc tcgtcgagga
     1921 acgctttggt ggtggcatca tcagtatcgt agtaagctcg tacccaggtg gagacgttct
     1981 ccagcgcctg gcgataagtc tcttcctggt gacgcggtac agcttgtgct gcgaccagca
     2041 ggcgagagcg aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg
     2101 cggtgtcatc acgacggcga atcgtaatga agttgtccat aaagttctgc cagctttttt
     2161 gcagattgat acgccattcg ctgatggaac tggaaagctc ttcaccgtcg gaatccatcg
     2221 gcgaaccatc gctgtcatta tcggccagac gcaggttatc tacctgattt gaaagctgat
     2281 taagcttaag gatgatgccg tcataatcca cctgcgaaac tgcagaaagg ctggcgatat
     2341 catcggtaat tgcccgacga acggtaatca gactcgggtc attcatatcc gccaggctgg
     2401 catctgcact tttcagcaac gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc
     2461 gtccggcgag tttcaccaga aaatcggcct gagccagcag ccaggtttta gcatcgctgc
     2521 cggaaatggt ggcgaccttt tgttggactt catccaactg ttttgccagc gtttcttgct
     2581 gacgattcgc ctgcttaagc tgtgcagctt gttgcttaat aatgccttcc agctcggctt
     2641 tttggctctc ctgggctttt tgcaatgccg tcagttggtt agccagggca tcgctggtgg
     2701 cggtctgatt gacggcctgt tgtttacccc agccatacaa accgacaccc gccgccagag
     2761 caatagcgat agccaccgcg ctgagaatca atgcagtatt gttcttactc tttttttctg
     2821 ttgcgacagg ttgtgacgtg gtgtccacgg cctccctggt ctcttcaacc acggcggagg
     2881 ttttttcttg ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa
     2941 gcgcatcgtt gtcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga
     3001 gtttcgccaa acgctcactg acgaccaata gtcgacagtg tagtaaccag tgctcacgat
     3061 accattgtgg gatcagcgac cagagttgct gcaacatttc accgctggta acaacgacca
     3121 tcgtcacctc gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc
     3181 atcgttgata acattcacaa aaagtgacct cagcaccgcg cgctgtcagg gtatccccaa
     3241 ttagctcacg accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta
     3301 attcaggtaa ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct
     3361 gtccgcttac ggtatgtagc gccagtgcgg tggtgcgtcc aatggcgaaa taatcaggta
     3421 gtcggggcca tttacgatct tgctgatgca gctgtgattg ggcaaaagca accgcgtgtt
     3481 gcgagagggc aaacaacaga tcgctctccc ccagcgcagc cagttgatca gcaagttgcg
     3541 gtaattgtcg acccggagaa aactcaatca gcggaaaatg ccaggccacc tgccccagtg
     3601 tgcgcagacg gctcactaac tcttctccag cgggagacgg gcgggtgacc aggatactca
     3661 tgccggggcg tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag
     3721 ctcttctgcc agcgaaatcc ccatttgttc ggcattttgc ggcgcaccgc ggcgttcacc
     3781 gcgaataatc tgcgaaccgt ccggcgcgcc gaccagcgca cgcagccaga tttcaccatc
     3841 aataagctcg gcgtagctac caattggcac ctgacatcca ccttcgagac gggtattcat
     3901 ggcgcgttct gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc
     3961 gcgagtgcgc gtatcatcaa ggcggcattc aataccgact gcaccttgtc ctaccgccgg
     4021 aagagaaatc tcgggtggta acgcggcgcg aatgcgggac tccagaccta aacgttttag
     4081 tccggctacg gcaagaatga tggcatcgta ttcgccgtta tctagtttgc tcaggcgagt
     4141 gccgacgttg ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca
     4201 ctggcgacgt aaactggacg tcccgacgat actgcctgcc ggtaacgcat ccagactgtc
     4261 ataattattg gacacaaagg catcgcgagg atcttcacgc tcacaaatag tgaccaaccc
     4321 cagaccttgc gggaattcaa ccggcacatc tttcattgaa tgtacggcga tatcggcgcg
     4381 attctccagg agcgcgactt ccagctcttt tacaaataag ccttttccgc ctactttcgc
     4441 cagcggcgta tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caaccaccag
     4501 gcccggatgg ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg
     4561 gctttggcgt gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat
     4621 ccgtggtcca tcctaacatc cttgccagag tgatgtcagt gttgtggtga aacgtagacg
     4681 cctgcgcaaa ccgtaaaatg aggtctggca gtggatcctg acaggcgttt cacgccgtcg
     4741 taataaggaa tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta
     4801 cggtcaatca gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact
     4861 aaaaaaacca ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg
     4921 ccgcgtgtta ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat
     4981 acgtcttgta cctctatatt gagactctga aacagagact ggatgccata aatcaattgc
     5041 gtgtggatcg cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc
     5101 cgacattgtt gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca
     5161 aaggcatttg cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac
     5221 tgtatcgtgg aatgtcagta caggacccac cgaaaggtga gcttccaatt actggtgtat
     5281 acaccatggg cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg
     5341 tctgtcatca atcctggctc gatagcgaag agcgccagtt actacaacgt aaatgtagtc
     5401 tgctggaaag ctgggccgcc tcgctgggtg tggaagtcag cttcttcctg attgatgaaa
     5461 accgcttccg tcataatgaa agcggcagcc tggggggcga agattgtggc tccacccagc
     5521 atatactgct gcttgacgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc
     5581 tgtggaatat ggtgccgtgc gacgaagaag agcattacga cgactatgtg atgacgcttt
     5641 acgcgcaggg cgtgctgacg ccaaatgaat ggctggatct cggtggctta agctcgcttt
     5701 ctgctgaaga gtactttggt gccagccttt ggcagctcta caagagtatc gattccccat
     5761 acaaagcggt actgaaaaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac
     5821 gtctgctggc gaaagatatc aaacagcgtt tgcacgacgg cgagattgta tcgtttggtc
     5881 tcgatccata ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt
     5941 ttacccgtct ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa aagctcagcc
     6001 gtgaacgcgc ctgcgtaggc tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg
     6061 gttgggacga agctcgtctg gcaatgctcg ataaccgcgc taactggaag attgatcagg
     6121 tgcgtgaggc gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgatcc
     6181 gctttgcgcg tcgcaataac cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga
     6241 cgcgtaagct gtatgccgcg tttgaagcat taccaggtaa agtgacgctg gtaaacccgc
     6301 agatttcacc cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccgggccgtg
     6361 ctaaccgttc aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc
     6421 atcagccgct ggaatataac cgttacctga acaaactggt ggcgtgggca tggtttaacg
     6481 gcctgctgac ctcgcgcacc cgtttgtata ttaaaggtaa cggcattgtc gatttgccta
     6541 agttgcagga gatggtcgcc gacgtgtcgc accatttccc gctgcgctta cctgcaccga
     6601 caccgaaggc gctctacagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg
     6661 aatatgaccc gacagcggcg ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg
     6721 atgtcttcag ctttggcgag aatcaaaatt gcctggtagg tagcgttgac ctgctgtacc
     6781 gcaactcgtg gaacgaagtg cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag
     6841 ccctgaaaac tattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag
     6901 tcttctgtta tagccagcat ctgcgcgggt taatccgtac tcgcgtgcag caactggttt
     6961 ctgagtgtat tgaactgcgc ctttccagca cccgccagga aaccgggcgt ttcaaggcgc
     7021 tgcgcgtttc tggtcaaacc tgggggttgt tcttcgaacg cctgaatgta tcggtacaaa
     7081 aactggaaaa cgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcttgt
     7141 cagtgcaggt tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgccagcg
     7201 aagggatcat ccagttcttc ttcgaagaaa cgcaagacga gaatggcttt aatatctaca
     7261 ttctcgacga aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc aaagaggagc
     7321 tggtacgtga cgtcagtcgc ttctactcgt catcgcacga tcgcttcacc tacggctcaa
     7381 gctttatcaa cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac
     7441 aggtgattcc gttccgcaca aaatctatcg gtaacatgcc gcccgccaat caggacaacg
     7501 atacgccgct attacagcag tatttttcgt gatgagcgtg ccggaaagcg aggcttatcc
     7561 ggcatgcaat cttagcggaa actgactgtt tcacccgctt gctgtgtcgc cgcctgttcc
     7621 agcaaatccc agaaggtttc gccgctgcga tcgcaaatcc attcatcgcc ttttaggtca
     7681 aaatggtagc cgccctgttt ggttgccagc cacacctggt gcagcggctc ctggcggtta
     7741 ataatgattt tgctgccgtt ttcaaaggta atggtcagta cgccgccgtt gatttcgcag
     7801 tcgatatcgc tgtcgccatc ccagtcgtcc aggcgttctt caatggtcag ccagagttga
     7861 tcagccaggc gatgaaattc actgtcgttc attgttgtat cctgtcttta agtgatggcg
     7921 gcagtatagc ggtatggggt cagggcttca aagtttgccc gtctgttgct gcgttccggc
     7981 atgagtcatc cgtcaacgga ataataatgt ctctgttgtg aagttcacgc acattctctt
     8041 ctctgacgtg tgttttcgcc gggcaggggg agggcgtaat ccttacctct gtatcggtcc
     8101 atgtataaac attgcgatcg tcataagcga agcgatcgtt tttagttagc ggaccgttaa
     8161 ctggaataat ttgcacgctc tttgaccgta attggcggat aaaataagca tgctgtttgc
     8221 cgctattaaa tgagcgatca atgaccaggt ctggcgtatc cagcgggatt atctcaagct
     8281 ggtcttcacc aaatgtcgat aattttgtta catacagccg atctttatat tgcgcaatat
     8341 tgccagtaat cagatggaat tgttcaggtt caatatctgg taggtctatc cacttgctcc
     8401 actgacggtc ggacgtgagc tgtttacgcc agcgaacgcg actttcccct acttcaaaag
     8461 agacgtcatc atctacgggt atcgctttgc cagacaattg cccaaaggga taacgatgca
     8521 tacccttatt gtcgcgaaaa accagcagtg aatgaggtat ccagcgaatt atctcaaatg
     8581 agtctgcatt cgccgtaagg tgaacgccat ttaaataaat attctgccca ctgcggataa
     8641 gcagatcatt ggaatacaaa tttctgttta cactcataag acgtggatta ataccccacc
     8701 atcttttttc taatacagta acattactgc tgctcccctg acgtcgaccg gacaaataga
     8761 gatttttact atccaccagc gtggtagagt taccttcgat agcttttagt gtcgccagat
     8821 caacacgtga cgcgccgcta ttactttcag tgcgctgtcc gtcaaaatag aggctatatt
     8881 tatcgaccgc gaagcggccc atagcctgaa agctggcaat atcgacagtg ggtgttcccg
     8941 gtggattgct aacgattttg ccacgccaga gaataacgcg gccgtctgtc acccagtggt
     9001 attcctcatt ttttatgctt atttcatcgg tgggtatata gccttccacc aactcccgct
     9061 ccccggatat atttgtcaca aggcgcaaat cggccctgct catctccgga aactgggcaa
     9121 tgattttctt atccccccaa taacctttac cgtaagacat aacaccgtga tcattgtaat
     9181 agtcgaaccc tatggcctgt acagcaggcg tcagaaatgt gtgtcccaac aggatgagtg
     9241 caactgtgat tatttgaccc ttcattttgt cgcctcattg cgcggggcag gaataaattc
     9301 gccgtcatca gtaatttttc cgtccagata tcccgttttg taatttacgc catagccata
     9361 tcccgaatgt tgttccttcc agattaagcg aacttgtttc ggatcagcgg tgcggtatcg
     9421 ccagagctgc gcgccatcaa agctgtaaat gtagcggtta tcgcgggcat accatctgcg
     9481 caaaaaggga tggtcattaa gatgggcttc gcgttcatcc ggagaacggt tatccagtaa
     9541 gatcagcggt ccgtcagatt caaacacatg taaaccggaa tggtatggcc acccttcggc
     9601 acgcgtaagt aaatagccgt ggcgtttccc ggcgttgaaa cgtttgttga taataagatt
     9661 gggatcatca agtttcacga tttccagctt gcggtcacca gactctgttt cgatgatggt
     9721 ataaagcgag tcctgatact gggcgacagc atcactgatg ggttgaaact gctccggatc
     9781 gagtccgggt aattcttcct gtttacaagc aggtccttta cgccagaaca ctttcttttc
     9841 cagcagagaa aaatccagac agtgctttgt taaatcttca tcccactcca gattttcctt
     9901 gtcgaggact ttacgctgca gcccgttttt atcacgccag gtcaagagtg agccgggcat
     9961 ccagcgcacg acggaaaagg tgtccgggtc ggcatcaagc tgttcgccgt tgatcaggat
    10021 tttggtccgt gtacgagcca gggtatccca ggggccaaat ggcatagcca cacagggatt
    10081 aaacgcggta gaaaactttc ctcgctgatc ccatgacttt tgtgccagca cacggaaact
    10141 atcgggatct gcgaggcgat gcccattgat atacaagaaa ttagcgtcac gtaatatcag
    10201 ccccagtaag tcaggatccc agggaggccg gaattccacc tgatgcagtg ttttgatatc
    10261 taagcgattt cccccgccat tgttatcggt gcgcttgcct tcgaaataga gactgtgttt
    10321 atctgcggca aaatcacccc acgccttgaa cgaggcgaca tcgacgggag gtttgtctgg
    10381 cgggttttgc acaacttcac ccgcgtagag gatatagcga ccatcgctat gccaggagta
    10441 atgaggatac caggcctgtg cgccttcata gaggtagtcg gttgtcagat cagtcagagg
    10501 aggtacttta tcccatttgt ctagtgttgt cgggtctatt aataaattgg gtaaacggcg
    10561 caaacttttg taattcacgt caggaagaga aagatttgtt tctttataag ggcgcaggta
    10621 taccacgcct tttccctgtt tctggtaaat attatgttca ggcggatcaa gccgacatcc
    10681 ccatgccgac gataaaaaca atgaacaaga caacaaacaa accacattgc gatagtgcat
    10741 aaagccatcc tggcgcgagg tgccgatcac gaaactacca gcagaacata attcctcgca
    10801 cgtaagcgta acttttgcgg caaatcgtca cttttcagaa tctataaagg ccatcgctat
    10861 tttctgatag ttgctggggt aaacttgagg ttttcattca cgatataaat acgagctact
    10921 gcgccatggt gtgactgatc acgttaaaat gtgccggatt gacgccggga agggtgtcaa
    10981 catgcgactc tgcccaccgt tacctacttt attgtctttc gcgtgttgta cttaggtccc
    11041 accaccggta tctaacattc cccacattat tacgctttct gccgaacgtg atttttatag
    11101 aaatctatca attcattttc cctgtaccat aaagtttatc tttcacatcc tgccgaacgc
    11161 gggtcttgta ccccccgttg tgaagatctt catacggaga aaaagaatct ttactccacc
    11221 ccagcagcac tttatcgcca ggcataaaat gcacatgcag aaaatcttct ccccattctc
    11281 ttttggggag aggtaacgtc atattccgtt tctccagacg cattccagca ttatactgtg
    11341 tacgagtagt acttaatgtc cagataacct ctgccgtatc cccactaaca tcaccacaac
    11401 aggttgttga accgccacca tttgcaaaag cattcgcgcc cgccacgccg ttaacactga
    11461 atcccagtat cgggcggtca atttcactgt ggatttccag aatcactcca cctcttggtc
    11521 ctccccatac tgcctgtcct atattccaca acacgaccag taccggaacg acaattaacg
    11581 tccgccatac ccagcgcccc caccgggcat aaccgcgcgt aagcttttgg tcaaattttt
    11641 ccagttgggt aaatagcccc atcagatttt attccctctt atcaccaggt tggcaaagtt
    11701 gtatgtcact ggcgactaag gtcagacaac aaaattgcat ggtgcgccac tcttttaaaa
    11761 gctccatgcc gacgataaga acaacgaaca agccaacaaa caaactacat tgcgatagtg
    11821 catagagcca tcctggcgcg aggtgccgat cacgaaacta ccagcaaaac ataaatcccc
    11881 acgagtaagc gttatactcg cagcatttcc tcacttttca gacttcataa agagtcgcta
    11941 aacgcttgct tttacgtctt ctcctgcgat gatagaaagc agaaagcgat gaactttaca
    12001 ggcaatccat aatgaaaaac gtgtttaagg cactcactgt attaattact ctcttcagcc
    12061 tgacgggctg cggtctgaaa ggtccgctct atttcccgcc tgcagataaa aacgcaccgc
    12121 cgccgaccaa accggtagag acgcaaacgc aatccacggt gccggataaa aacgatcgcg
    12181 ccactggcga tggtccatcc caggtgaatt actaaaagtc agtttctgta cccgcgtgat
    12241 tggagtaaat gatgcagttc tcgaaaatgc atggccttgg caacgatttt atggtcgtcg
    12301 acgcggtaac gcagaatgtc tttttttcac cggagctgat tcgtcgcctg gctgatcggc
    12361 acctgggggt agggtttgac caactgctgg tggttgagcc gccgtatgat cctgaactgg
    12421 attttcacta tcgcattttc aatgctgatg gcagtgaagt ggcgcagtgc ggcaacggtg
    12481 cgcgctgctt tgcccgtttt gtgcgtctga aaggactgac caataagcgt gatatccgcg
    12541 tcagcaccgc caacgggcgg atggttctga ccgtcaccga tgatgatctg gtccgcgtaa
    12601 atatgggcga acccaacttc gaaccttccg ccgtgccgtt tcgcgctaac aaagcggaaa
    12661 agacctatat tatgcgcgcc gccgagcaga caatcttatg cggcgtggtg tcgatgggaa
    12721 atccgcattg cgtgattcag gtcgatgatg tcgataccgc ggcggtagaa acgcttggtc
    12781 ctgttctgga aagccacgag cgttttccgg agcgcgccaa tatcggtttt atgcaagtgg
    12841 ttaagcgcga gcatattcgt ttacgcgttt atgagcgtgg ggcaggagaa acccaggcct
    12901 gcggcagcgg cgcgtgtgcg gcggttgcag tagggattca gcaaggtttg ctggccgaag
    12961 aagtacgcgt ggaactcccc ggcggtcgtc ttgatatcgc ctggaaaggt ccgggtcacc
    13021 cgttatatat gactggcccg gcggtacatg tctacgacgg atttattcat ctatgaagca
    13081 accaggggaa gaactgcagg aaacactcac ggagcttgat gaccgggcgg ttgtcgatta
    13141 tctgattaaa aatcctgagt tttttatccg taatgcgcgc gcagtagaag cgatacgtgt
    13201 gccgcatccg gtacgcggca ccgtttcgtt ggtcgagtgg cacatggccc gcgcacgtaa
    13261 tcatattcat gttctggaag agaacatggc gctgttgatg gaacaggcta tcgccaacga
    13321 aggcctgttt tatcgcctac tctacctgca gcgcagtctc accgccgcca gcagtctcga
    13381 cgatatgctg atgcgctttc accgctgggc gcgcgatctc ggcctggcag gtgcgagtct
    13441 gcgcctgttt ccggatcgct ggcgcttagg tgcgccgtcg aaccacactc atctggcatt
    13501 aagccgtcag tctttcgaac cgctgcgtat tcagcgtttg gggcaggaac agcactatct
    13561 tgggccgctt aacggaccag agctgctggt ggtgctaccg gaagcgaaag cggtgggatc
    13621 ggtggcgatg tcgatgctgg gaagcgatgc tgatttgggt gtcgtgctgt ttaccagtcg
    13681 cgatgccagt cactatcaac aagggcaagg aacgcagtta cttcatgaaa ttgcgctgat
    13741 gttgccggag cttctggagc gttggattga acgcgtatga ccgatttaca caccgatgta
    13801 gaacgctacc tacgttatct gagcgtggag cgccagctta gcccgataac cctgcttaac
    13861 taccagcgtc agcttgaggc gatcatcaat tttgccagcg aaaacggcct gcaaagctgg
    13921 cagcaatgtg atgtgacgat ggtgcgcaat tttgctgtac gcagtcgccg taaagggctg
    13981 ggagcagcaa gtctggcgtt acggctttct gcgctacgta gcttttttga ctggctggtc
    14041 agccagaacg aactcaaagc taacccggcg aaaggtgttt cggcaccgaa agcgccgcgt
    14101 catctgccga aaaacatcga cgtcgacgat atgaatcggc tgctggatat tgatatcaac
    14161 gatcccctcg ctgtacgcga ccgtgcaatg ctggaagtga tgtacggcgc gggtctgcgt
    14221 ctttctgagc tggtggggct ggatattaaa cacctcgacc tggagtctgg tgaagtgtgg
    14281 gttatgggga aaggcagcaa agagcgccgc ctgccgattg gtcgcaacgc tgtggcgtgg
    14341 attgagcact ggcttgattt gcgcgacctg tttggtagcg aagacgacgc gctttttctg
    14401 tcgaaactgg gcaagcgtat ctccgcgcgt aatgtgcaga aacgctttgc cgaatggggc
    14461 ataaaacaag ggctgaataa tcacgttcac ccacataaat tacgtcactc gttcgctacg
    14521 catatgctgg agtcgagcgg cgatcttcgt ggtgtgcagg agctactggg tcatgccaac
    14581 ctctccacca cgcaaatcta tactcatctt gattttcaac accttgcctc ggtgtacgat
    14641 gcggcgcatc cacgcgccaa acgggggaaa taatgcgttt ttaccggcct ttggggcgca
    14701 tctcggcgct cacctttgac ctggatgata ccctttacga taaccgtccg gtgattttgc
    14761 gcaccgagcg agaggcgctt acctttgtgc aaaattatca tccggcgctg cgcagcttcc
    14821 agaatgaaga tctgcaacgc ctgcgccagg cggtacggga agtggaaccc gagatttatc
    14881 acgacgtgac gcgctggcgt tttcgttcga ttgaacaagc gatgctcgac gccgggctga
    14941 gtgccgaaga agccagtgca ggcgcacacg cagcaatgat caactttgcc aaatggcgca
    15001 gccgaatcga cgtcccgcag caaactcacg acaccttaaa acagctggcg aagaaatggc
    15061 cgctggtggc gatcaccaac ggtaacgccc agccggaact gtttggtttg ggggattatt
    15121 ttgagtttgt gctgcgcgct ggcccgcacg ggcgctcaaa accgttcagc gatatgtact
    15181 ttctggctgc ggaaaaactc aacgtgccga tcggcgagat cttacatgtt ggggacgatc
    15241 tcaccactga cgtgggtggg gcaattcgca gcggaatgca ggcctgttgg atcagaccgg
    15301 aaaatggcga tctgatgcaa acctgggaca gccgtttact gccgcatctg gaaatttccc
    15361 ggttggcatc tctgacctcg ctgatataat cagcaaatct gtatatatac ccagcttttt
    15421 ggcggagggc gttgcgcttc tccgcccaac ctatttttac gcggcggtgc caatggacgt
    15481 ttcttacctg ctcgacagcc ttaatgacaa acagcgcgaa gcggtggccg cgccacgcag
    15541 caaccttctg gtgctggcgg gcgcgggcag tggtaagacg cgcgtactgg tgcatcgtat
    15601 cgcctggctg atgagcgtgg aaaactgctc gccatactcg attatggcgg tgacgtttac
    15661 caacaaagcg gcggcggaga tgcgtcatcg tatcgggcag ctgatgggca ccagccaggg
    15721 cggcatgtgg gtcggcacct tccacgggct ggcgcaccgt ctgctgcgtg cgcaccatat
    15781 ggacgccaat ctgccgcagg attttcagat cctcgacagc gaggaccagc tacgcctgct
    15841 caaacgccta atcaaggcga tgaacctcga cgagaagcag tggccgccgc gccaggcaat
    15901 gtggtacatc aacagccaga aagatgaagg cctgcgtcca catcatattc aaagctacgg
    15961 taatccggtg gagcagacct ggcagaaggt gtatcaggcg tatcaggaag cgtgtgaccg
    16021 cgcgggcctg gtggacttcg ccgagctgct gctacgtgct cacgagttgt ggcttaacaa
    16081 gccgcatatc cttcagcact atcgcgaacg gtttaccaat atcctggtgg acgaattcca
    16141 ggataccaac aacattcagt atgcgtggat ccgcctgctg gcgggcgata ccggcaaagt
    16201 gatgatcgtt ggcgatgacg atcagtcaat ctacggctgg cgtggagcgc aggtggagaa
    16261 tattcagcgt ttcctcaatg atttccccgg tgctgaaact atccgtctgg agcagaacta
    16321 ccgctctacc agcaatattc tgagcgccgc gaacgccctg attgaaaaca acaacgggcg
    16381 tttgggtaaa aaactgtgga ccgatggcgc ggacggtgag cctatttccc tctattgcgc
    16441 ttttaacgaa ctcgatgaag cgcgttttgt ggttaaccgc atcaaaacct ggcaggacaa
    16501 cggcggggcg cttgccgagt gcgccattct ctaccgcagt aacgcccagt cgcgtgtgct
    16561 ggaagaagca ctgttgcagg cgagtatgcc gtaccgtatt tacggcggga tgcgcttctt
    16621 cgaacgccag gaaatcaaag atgcgctctc gtatctgcgc ctgattgcca accgtaacga
    16681 cgacgcggcc tttgagcgtg tggtgaatac gccaacgcgg ggtattggtg accggacgct
    16741 ggacgtggta cgtcagacat cgcgcgatcg ccagttaaca ctctggcagg catgtcgtga
    16801 actgttgcag gaaaaagccc tcgccggacg tgctgccagc gccttacagc ggtttatgga
    16861 actgatcgac gccttagcgc aggaaactgc cgatatgccg ctgcatgtac agactgaccg
    16921 ggtaattaaa gactccggcc tgcgtaccat gtacgagcag gagaagggcg aaaaaggtca
    16981 gacgcgtatc gaaaacttag aggaactggt gacggcaacg cgccagttca gctacaacga
    17041 agaagacgaa gatttaatgc cgctgcaggc gttcctatcc catgcggcgc tggaagcagg
    17101 cgaagggcag gcggatacct ggcaagatgc ggtacaactg atgaccatgc actcggcgaa
    17161 aggtctggag ttcccgcagg tgtttatcgt cggtatggaa gagggcatgt tcccaagcca
    17221 gatgtcgctg gatgaaggcg ggcgtctgga agaagaacgc cgtctggcct acgttggcgt
    17281 aacccgcgcg atgcagaaac tgacgctgac ctacgcggaa actcgccgtc tgtatggcaa
    17341 agaggtttac catcgcccgt cgcgctttat tggcgagctg ccggaagagt gtgtggaaga
    17401 ggtgcgcctg cgcgccacgg taagccgccc ggtcagccat cagcggatgg gtacgccgat
    17461 ggtcgagaac gacagcggct acaagctcgg ccagcgcgta cgccacgcta agtttggtga
    17521 aggcaccatt gtcaatatgg aaggcagcgg tgagcatagc cgtttgcagg tggcatttca
    17581 gggccagggt attaaatggc tggtggcggc ttatgcccgg ctggagtcgg tgtaacgttg
    17641 ccggatgcgg tgctgcgcac cttacttggc ctaaaaaatc attcagattc aataaattgc
    17701 aacgtcatgt aggccggata gggcgtttac gccgcatccg gcatctgcgc catcttcagt
    17761 atctgacaca aaactatcgt tttaaccttt ccgctctacg gaaatcatgg tgacaaaagg
    17821 atagcgttgc cacgggattg ccccgccttt catatacata tgtgaaatcg tgccatcaag
    17881 ataaagcagc tgctcaacgt tcagtttcgc tttggcataa caggcaaaat cataaaaatt
    17941 tgtcgcctgc tggctcaaca aaaacacggc attcccatgt ttattaatcc caacaccgtt
    18001 acgaatttta cgtgaggcga cgttgggatg aatacgcgga ttaattacac tgttttccat
    18061 caacattggc cctgactgca ccgcaaactg aatctcttta ctggttttga aggcatccag
    18121 acgaacgatg ccgactttat ctcccgcgac ataaaacacg ccgccaggac ggataaagaa
    18181 attcccttca cctgaagcga gatttaacgc caccttctgc tgaccgtttt cgatgtacaa
    18241 accgagcggc gcatagcttt catcatagat gccgccgttc atcgccatct gcacctgacc
    18301 ctgactattc atatccgcca gcagagcatg taaagttccc cacgcttcgc cattggcttt
    18361 ttgccagtac attttcaccc gctctgtttg aggattaacg gtatacgcct gtacggtcag
    18421 cgtcggatct gagagtgcgc aatcatcagc ggcaacagca aacaagggaa gtaaggtgag
    18481 ggtgagaaaa atccgtttga gattcaaggt gatcattcct ttaccaatga gtagccgatg
    18541 cgccattata ggtcctggat gttggatttt tttatcctgt tagcgacctt gacgagtacc
    18601 aaaaagcgcg aagttcaact attgttctgt ggtgttctgt tgcgtgttga cggcaaaatt
    18661 ttgctggcgt aacatgcgcg cacgatcact ctaagaggac attcgccttg gacacaccca
    18721 gtagatactg gctcactatc ctgtcatcca ggatcaactc ctaaggctat ccctttttgc
    18781 tgatagcctt agcggttgtc agcgacctca atttttcccg tcgcgctgag tcaggctgtt
    18841 taatggtctg aaacccaatt tgtttctgtg tgcccaccga actgtccgat attttaagca
    18901 ttgggagtcc cggtcatgct gagcgcattt caactggaaa ataaccgact gacccggctg
    18961 gaagtcgaag agtcacaacc ccttgtaaat gcagtatgga ttgatcttgt cgaaccggac
    19021 gacgacgagc gactgcgcgt acaatctgaa cttggccaga gcctggcaac ccgcccggaa
    19081 ctggaagaca tcgaagcatc ggcacgtttc tttgaagacg acgacggcct gcatattcac
    19141 tccttcttct tctttgaaga tgcggaagat cacgccggta actccactgt ggcatttacc
    19201 atccgtgatg gtcgtctgtt tactctgcgt gagcgtgaac tgcccgcttt tcgtctgtat
    19261 cgtatgcgtg cccgtagcca gtcgatggta gacggtaacg cctacgagtt gctgctggat
    19321 ctgttcgaaa ccaaaatcga acagttggca gatgaaattg aaaatatcta tagcgacctg
    19381 gagcagttga gccgggtgat tatggaaggg catcagggcg atgagtacga cgaggcgctc
    19441 tccacgctgg cggaactgga agatatcggc tggaaagttc gcctgtgtct gatggatacc
    19501 cagcgcgcgc tcaacttcct ggtacgcaag gcgcgtttac cgggtgggca actggagcag
    19561 gcgcgtgaaa tcctgcgaga tatcgaatcc ctgctgccgc ataacgaatc cctgttccag
    19621 aaggtgaact tcctgatgca ggcggcgatg ggctttatca acatcgagca gaaccgcatc
    19681 atcaaaatct tctcggtggt atccgtggtg ttcctgccac cgacgctggt ggcttccagc
    19741 tacgggatga actttgaatt tatgccagag ttgaagtgga gtttcggcta cccaggcgcg
    19801 attatcttta tgatcctcgc gggcctggca ccgtatctgt actttaagcg gaagaactgg
    19861 ttgtaatatg agtgccggat aaagctattt ttttatccgg cttatttcta aaaattgtct
    19921 tttttaaagt catgcggatt gaaaactcgc tctattttat tttcttgtgt taattgatac
    19981 tcagtaaaat cgttaatacc aatatgagtg ttacgataag ggtattcatt aataaccatt
    20041 aagtaatttt tcacgatgca attatcattg ctcttaattg tcgggaaaca gtagagatct
    20101 tcatacggcc cttcgatata tttttttata gtcagtgaag ccattaccga tatcaatgac
    20161 ataccaggta tgccaactac cttctggtag gggatgttga tttttaattt cattctgatg
    20221 cttcagatac caatttattt tatcgctgtc agtccatggc agatgatcaa cgaccagaaa
    20281 gactgaattg ccagcatcag agtatgtata caaaatctgt acaggacgca atgtccataa
    20341 attatagatg cagcatagaa gaaataaaca taatataatt ttctggagtt tgcttttcat
    20401 aga
//
LOCUS       DAZFRJ010000071        19990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000071 DAZFRJ010000000
VERSION     DAZFRJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.071"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(166..912)
                     /gene="cutC"
                     /locus_tag="ACHKUN_002375"
     CDS             complement(166..912)
                     /gene="cutC"
                     /locus_tag="ACHKUN_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO6660623.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(926..1492)
                     /gene="yecM"
                     /locus_tag="ACHKUN_002376"
     CDS             complement(926..1492)
                     /gene="yecM"
                     /locus_tag="ACHKUN_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6660624.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1708..3441
                     /gene="argS"
                     /locus_tag="ACHKUN_002377"
     CDS             1708..3441
                     /gene="argS"
                     /locus_tag="ACHKUN_002377"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO6660625.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3483..3527)
                     /gene="yecV"
                     /locus_tag="ACHKUN_002378"
     CDS             complement(3483..3527)
                     /gene="yecV"
                     /locus_tag="ACHKUN_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO6660626.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3618..4106
                     /gene="yecT"
                     /locus_tag="ACHKUN_002379"
     CDS             3618..4106
                     /gene="yecT"
                     /locus_tag="ACHKUN_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO6660627.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4226..4618)
                     /gene="flhe"
                     /locus_tag="ACHKUN_002380"
     CDS             complement(4226..4618)
                     /gene="flhe"
                     /locus_tag="ACHKUN_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO6660628.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4618..6696)
                     /gene="flhA"
                     /locus_tag="ACHKUN_002381"
     CDS             complement(4618..6696)
                     /gene="flhA"
                     /locus_tag="ACHKUN_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO6660629.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6689..7837)
                     /gene="flhB"
                     /locus_tag="ACHKUN_002382"
     CDS             complement(6689..7837)
                     /gene="flhB"
                     /locus_tag="ACHKUN_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO6660630.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8039..8683)
                     /gene="cheZ"
                     /locus_tag="ACHKUN_002383"
     CDS             complement(8039..8683)
                     /gene="cheZ"
                     /locus_tag="ACHKUN_002383"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO6660631.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8694..9083)
                     /gene="cheY"
                     /locus_tag="ACHKUN_002384"
     CDS             complement(8694..9083)
                     /gene="cheY"
                     /locus_tag="ACHKUN_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO6660632.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9098..10147)
                     /gene="cheB"
                     /locus_tag="ACHKUN_002385"
     CDS             complement(9098..10147)
                     /gene="cheB"
                     /locus_tag="ACHKUN_002385"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO6660633.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10150..11010)
                     /gene="cheR"
                     /locus_tag="ACHKUN_002386"
     CDS             complement(10150..11010)
                     /gene="cheR"
                     /locus_tag="ACHKUN_002386"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO6660634.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRCFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11029..12633)
                     /gene="tap"
                     /locus_tag="ACHKUN_002387"
     CDS             complement(11029..12633)
                     /gene="tap"
                     /locus_tag="ACHKUN_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO6660635.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12679..14340)
                     /gene="tar"
                     /locus_tag="ACHKUN_002388"
     CDS             complement(12679..14340)
                     /gene="tar"
                     /locus_tag="ACHKUN_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO6660636.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14485..14988)
                     /gene="cheW"
                     /locus_tag="ACHKUN_002389"
     CDS             complement(14485..14988)
                     /gene="cheW"
                     /locus_tag="ACHKUN_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO6660637.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15009..16973)
                     /gene="cheA"
                     /locus_tag="ACHKUN_002390"
     CDS             complement(15009..16973)
                     /gene="cheA"
                     /locus_tag="ACHKUN_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO6660638.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16978..17904)
                     /gene="motB"
                     /locus_tag="ACHKUN_002391"
     CDS             complement(16978..17904)
                     /gene="motB"
                     /locus_tag="ACHKUN_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO6660639.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17901..18788)
                     /gene="motA"
                     /locus_tag="ACHKUN_002392"
     CDS             complement(17901..18788)
                     /gene="motA"
                     /locus_tag="ACHKUN_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6660640.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(18915..19493)
                     /gene="flhC"
                     /locus_tag="ACHKUN_002393"
     CDS             complement(18915..19493)
                     /gene="flhC"
                     /locus_tag="ACHKUN_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO6660641.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19496..19846)
                     /gene="flhD"
                     /locus_tag="ACHKUN_002394"
     CDS             complement(19496..19846)
                     /gene="flhD"
                     /locus_tag="ACHKUN_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO6660642.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4764 a         5519 c         4892 g         4815 t
ORIGIN      
        1 aaaaatatta ttagcacaac gctatgttca ctgtcacccg ctcaacagat cacggttatc
       61 gttcgttttt tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg
      121 gtacgagcaa gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg
      181 gcgttcaatg attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata
      241 ctcgtccgcg tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga
      301 ggcttgccac gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg
      361 caggttttct gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat
      421 aagttccata atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac
      481 ccgggcaatg cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc
      541 gaaggcgcga tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg
      601 tggcatatcg acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa
      661 ttcgcggacc gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa
      721 atcaccaccg cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga
      781 tttcagtacc cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac
      841 tctgtctgcg ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc
      901 cagtaatgcc atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa
      961 tggaccacgg atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg
     1021 gtaatcgctc atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga
     1081 gtccttcatc agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggca
     1141 gaacgatttc aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta
     1201 gctccacaat agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac
     1261 aaatcggtct gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc
     1321 gacgccagcg ttcagcagtg gcgttttgat ggcaacgcaa agaaatgtga tcggcagtca
     1381 gcggagtgat attcaacccc agacggaggg aaagttcatc taatgcgtgg gtaaatcgcg
     1441 gtaaatccga tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc
     1501 ttcgtctttc agtaaaagcg ttaatttacc ctgttgccct gcgccaacca accgctgatt
     1561 tcacgccgct tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga
     1621 aaacagccat ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg
     1681 ctcgcttcat caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc
     1741 cgtcaggcca tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca
     1801 gcaaaagttc agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg
     1861 ggtatggcac cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc
     1921 gccagcaaag ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc
     1981 ctggctgaac atgttcagca ggcgctggcg tccgatcgtc tcggtgtttc tacgccagaa
     2041 aaacagacca ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcatgtcggt
     2101 cacctgcgct ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac
     2161 aaagtgattc gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca
     2221 tggctggaaa agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt
     2281 ttctaccgcg atgcgaaaaa gcattacgac gaagatgaag agttcgccga gcgtgcacgt
     2341 aactacgtgg taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg
     2401 gtcgacatca ccatgacgca gaaccagatc acttacgatc gtctcaacgt gacgctgacc
     2461 cgtgatgacg tgatgggcga aagcctctac aacccaatgc tgccaggaat tgtggcggat
     2521 ctcaaagcca aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt ccttgatgag
     2581 tttaaaaaca aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat
     2641 ctctacacca ccactgatat cgcctgtgcg aaatatcgtt atgaatcact gcatgccgat
     2701 cgcgtgctgt attacatcga ctcccgtcag catcaacacc taatgcaggc atgggcgatc
     2761 gtccgtaaag caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg
     2821 atgctgggta aagacggcaa accgttcaaa acccgcgcgg gtggtacagt gaaactggcc
     2881 gatctgctgg atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa gaacccggat
     2941 atgccagctg acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat
     3001 gcggatctct ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg
     3061 tttgagggta ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt gtccgtgttc
     3121 cgtaaggcag aaattaacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat
     3181 cgtgaagcgc aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc
     3241 cgtgaaggca cgccgcatgt gatgtgtgct tacctgtacg atctggctgg tctgttctct
     3301 ggcttctacg agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt
     3361 ctgaaactgg cacaactgac agcgaagacg ctaaagctgg gtctggatac gctgggtatt
     3421 gagacagtag agcgtatgta atcgattttt cgtgagagta aagcctgatc agggttacct
     3481 gatcaggctt ttttattgcc atctaaatgt attctgaaaa tggacatgcc attgttttct
     3541 gactgttgga taagaggcca gaagcgtaat atccggcccc agggaaacga taacggttga
     3601 atttaaggaa taccgcagtg tttaaatttc ttgtattaac attaggcatt atctcttgcc
     3661 aggcttacgc agaagatacg gttatagtaa acgaccatga catttcagcc atcaaagatt
     3721 gttggcaaaa aaattcagat gatgatactg acattaacgt gatcaaatca tgcctgcgac
     3781 aagaatacaa tctcgtcgat gcgcaattaa ataaagccta tggtgaagct tatcgttata
     3841 tagaacaagt gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt aacttgctta
     3901 aaaaatcaca gcgagcctgg ctggatttta gggacaaaga atgtgaatta atcctttcaa
     3961 atgaggacgt tcaggattta agtgaccctt attctgaatc agaatggctc tcatgtatga
     4021 tcatacagac caatacgcga actcgccagt tgcagctata ccgtaactct gaagattttt
     4081 atccaagccc tttgacaaga ggataattca catctttttg gcatgttttg ttgcaagcta
     4141 ttcctgataa ataattgcaa caagacatcg agcctttttc actgagttat taaacatact
     4201 cgcgagcgcg taattttttt gtccttcagc gataattcac aatcacttca ttacgttgta
     4261 cctttagcgg tggaattaac cgaccaccgc ctggcacttc ccagataaat cgcaacggtt
     4321 ctgctgccgc tataccggaa aaggccacgg tggttccgct ctgcccctct aattcgacac
     4381 aacgagattg cgaacacaag cgaacccgca gtcctgaagg tgtcgggccg ataagctgat
     4441 aacgccacgc taccagcgtc atcaatcctg aagcaggttg tcgcgtagaa agaggcgcag
     4501 acgacatcga ctcaccgcga tgatttagcg taattcctac actacttgcc tgccacatcc
     4561 cctccccggc ggcttgcacc agcagcggaa acaataatat tgctaataag gttctcatta
     4621 tttgccgcca attgtcgccg tcatgcggat atgtcggtta tcagacagtt ccagattcga
     4681 caggaccact aactgcggca agctgcggcg caggaagcga gacaataatg gtcgcggcgc
     4741 gtggttcacc aacaatactg gcggcgcacc cagcatctcc tgacgggata gcgcttcctg
     4801 agtttgcgcc agtaaacgat ccgccagccc tggctccagt cctcccccgc cctgcagcgc
     4861 ctgtagtaac aaacgttcca gcggtgtatc gaggccaata acatggactt catctttgcc
     4921 aggaaaccac tgctgggtaa tcgcccgtcc caacgccacg cgcacgacgg cggttaattc
     4981 atgtggatcg ctttggatgg gcgcatgttc cgccagcgtt tcgagaatgg tgcgcatatc
     5041 gcgaatcggt actttttcat cgaggagatt ttgcagcact ttatgcagtg tggtgagcgt
     5101 gacgacgcca ggaacgagat cttccgtcag ctttggcatc tcctgggcga cgcgatccaa
     5161 cagctgttgc gcctcctgac gaccaaacag ctctgcggca tgctggctaa tgaggtggtt
     5221 aagatgcgtt gctaccaccg tgctggcctc aaccactgtg tacccctgaa tctgcgcctg
     5281 ttcttttagc gcactttcaa tccagatagc attcaggcca aatgccggat cgacggtcgc
     5341 ctcaccaggt aacgtcccgg cagcggttcc agggttaatc gccagccagc gccccggata
     5401 agcatcacca ctgccaatct ccacgccttt catcaaaatg cgatagcggg caggttgcag
     5461 ctccatattg tcgcgaatgt gcaccactgg cggcagaaat cccatctcct gggcaaattt
     5521 cttgcggata ctgcgtatac ggcccaacaa ctcaccatcc tgctggaaat cgaccatcgg
     5581 gatcagtcga taacccactt ccattcccag agaatcttcc agttgtacat cgttccacgt
     5641 cgcttcgaca acggtattat tctctgccat ttttaccggt ttgggttcga caggcgcttt
     5701 ttgttcgcgt ccgcgtatcc accaggccag ccccagcaat ccggcagtga acagcaaaaa
     5761 taccaggttc ggcattccag gcaccaggcc gagtaaaccg agcacggcgg cgcttaacaa
     5821 cataacgctt gggttactga aaagctgatt caccatctgc tcgccaacat cctgatcggt
     5881 gctgacacgc gtaacgatga cccccgcggc ggtagaaatc accagcgccg gaatttgtgc
     5941 caccagaccg tcgccaatgg tcaatagcgt ataactttcc gccgcgtgtc ccatgctcat
     6001 gccatgttgc agcacgccga ccagcaaccc gccgacaacg ttaatgacca tgatgaggat
     6061 cccggcgatg gcatcgccgc gaacaaactt acttgccccg tccattgagc cgtaaaaatc
     6121 ggcttcctga gtcacttcgg agcggcgttt tttcgcctca tcttcaccaa tcaatccagc
     6181 gttaaggtcg gcgtcaatcg ccatctgctt acccggcata ccatcgagaa caaagcgcgc
     6241 acccacttct gcgatacgcc cggcaccttt ggtaatgacc ataaagttga tgatcacgag
     6301 aatgacaaac actacgatac cgatagcgaa attgccacca acgaggaagt gaccgaatgc
     6361 ttcgaccacc ttcccagccg ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt
     6421 tgaagccacg ttaagtgcca gacgcaacaa cgtagtaaac aacagaatgg tcggaaacgc
     6481 agcaaactca agcgtgcgct gggtaaacat cgccaccagc aacaccatga tcgacaaggc
     6541 aatattgaag gtaaacaaca ggtcgagtat gaatgcgggc agtggcagca ccatcatcga
     6601 caagatcaac aggatcaaaa tcggtccggc aaggatctgc cattgtgtcg atttcaggtt
     6661 tgcgggcagg cgcagcatcg cggccagatt actcatgggt cggtttctcg ttaataaaat
     6721 ccagggcttc cggcaccgga agatgagtag gttgtacagg gcgctgtcca ccagccagac
     6781 gccagcgttt cagttgccag acccaggcca gcacttccgc caccgcggcg tacagttgac
     6841 ccgggatttg ttgaccaatc tccgcatgtc gatacagcgc tcgcgccagc ggcggcgctt
     6901 caagcgtcgg gacgttattt tcagcgccaa tttcacgaat gcgcagcgcg accagccctg
     6961 cacctttagc gaccactttc ggtgcgctca ttttgttttc gtcatactgc aacgctaccg
     7021 aatagtgggt cgggttattg acaatgacat ccgctttcgg cacatcggcc atcatccgac
     7081 gccgtgcagc agctcgctgc atctgacgga tccgcccttt aacatgaggg tcaccttcgc
     7141 tttgtttgaa ctcatcacga atatcctgcc gtgacatacg cagctttttc aggtggctga
     7201 atatttggaa aaagacgtca aatcccacca ttggaatgac accaagcacc accagcagtg
     7261 cgcatagccc taccaaatcc atcgcattac ccatggcggt aatcggagac tcggccatca
     7321 agcgcatcat ctgcggccag tgatgccaga gaaaaaaccc cgtcacgctg ccaaccagga
     7381 tggttttcaa aattgcttta agcaactccg cgccagtctg agccgagaac atccgtttaa
     7441 tgcccggtag cgggttgagt ttggaaaact tcggctgcaa ggatttgccg ctaaatacca
     7501 gccctcccag catgaccgga gaaatgagcg ccaccagcac cacgccgcta atcagcggca
     7561 gcagcgccag catggcttct ctgatcagca gaataatctg cccgaggatc agattcgggt
     7621 cattgataat actgtgatca aaatgcagcc cagcggagag catgcccgac aatcgacggg
     7681 ccagcgacac accgccaaac cagataacac taacgcccac cagcaaaatc agcagtgagg
     7741 tcagttcacg ggaacgcggg atttgcccct cttcccgcgc tttttctagt cggtgaggtg
     7801 tgggggcttc tgttttgtcg tcgctctcgt cagacacgtc gccaatcctg gaaagagtat
     7861 taagcgtgaa tgatgccaga gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg
     7921 cgggatttag ggcttttgct gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg
     7981 tcatcgcctt atcagaccgc ctgatatgac gtggtcacgc cacatcaggc aatacaaatc
     8041 aaaatccaag actatccaac aaatcgtcca cctgatcctg actggctacc acaccggctt
     8101 tgctggtatc gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact
     8161 cctgttccgg gatgttttcc aacagcacca tcagcaactg gcgttcgatc tcctgaatga
     8221 catccatcat ccgcttaatg acctgcccgg tgagatcctg aaaatcctgc gccatcatga
     8281 tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc
     8341 gtgtatctgt taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat
     8401 cccaacgttg ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg
     8461 cctcaacact gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca
     8521 aacgatcgcg cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca
     8581 gttcccgcaa actgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc
     8641 cagctgaatg ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg
     8701 cccagtttct caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc
     8761 accacatagc cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct
     8821 gcagtcacca ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc
     8881 aattccaggc catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct
     8941 gcctgcaact tattgagagc gtcgacgcca tcttccgctt cctcaacatt attgaatccc
     9001 agctctttca gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa
     9061 aatttaagtt ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg
     9121 cactaatttt tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac
     9181 cacccatatt gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg
     9241 caagggtcca tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca
     9301 taccggtcag gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca
     9361 acacatctac cgaaggccga tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt
     9421 aatttgcgcc actacgcgac agctccatat gccgatcgcc cggcgcaata taggcatgcc
     9481 ccggcaagac acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa
     9541 gtctgtcggc aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg
     9601 ggctggaaag cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag
     9661 caccaatcgc aatcagtttt tcagaactca acaacggacc cgccttcagc gttgtcggtg
     9721 ctgacaatgg cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa
     9781 tcatttcgct atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat
     9841 ctattgcccc cagctccagc gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag
     9901 aaaccatcac aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt
     9961 ccatccgcgg catttcaaca tccagcgtca gcacatcggg attgaatttc ttaatcaagt
    10021 cacgcgcgac cagcggatca ggcgcggtcg ccaccatttc catgtcgcta tggctgttga
    10081 tgatttctgt catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt
    10141 tgctcatcgt taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg
    10201 ctcaaggtgg ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag
    10261 cggaacaaag cagcgcaaaa tctcctgctg ggtattttga tcgaagtaga tcatgacgtt
    10321 acgacagaag atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gtagattcag
    10381 cggggcaaaa tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg
    10441 tcccgtccct cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc
    10501 atggcgataa ataccgcttc tggctttttc cagcacttcg gtgtcgatat cactggcaaa
    10561 cactttccag cgtccgggcg cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta
    10621 cggctcttcg ccggtcgaag ccgccgcgct ccatacgcga tactcgccag aacggcgacg
    10681 tgcgtgatcc gcgagcagag ggaaatggtg tgcctcacgg aaaaatgccg tcagattcgt
    10741 ggtcagcgaa ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt
    10801 cagataatga ccgaaatccg tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta
    10861 aaccatgtcg cgtttatggt cagccagaac gatcccggct cgttgataga tcaattgact
    10921 tatccgccga aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga
    10981 cgtttgccca cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac
    11041 tggcgcaatt tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa
    11101 taccgccaca cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg ttgccaccgc
    11161 cgcttcttct accagcgagg cgttctgctg agtcacctga tccatctggc tgacagcctg
    11221 tgcaacctgc tcaatccccc gctgttgttc ttccgacgca gaggcaattt ctcccataat
    11281 gtcgtccacg cgggtcaccg aactgacaat atcgatcatg gtcgcggcgg cgttattcac
    11341 cagtttcgaa ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt
    11401 tgctgcctgc gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc
    11461 acgcccctgc tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt
    11521 ctggaaagca attccgtcga taacgctgat aatgtcgcca attttttgcg agctggtggc
    11581 gatctcctgc atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt
    11641 cgcggcattt tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt
    11701 ggcggttaat tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt
    11761 acgacttgag agatcgttat tgcctgcgac aatctccgcg ataccaatgt gcatctcctg
    11821 gcttccctta cgcacatcac tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct
    11881 ggcaaaaatg gcggtgatct cattacgacc atataccgca atcggacgcg ccagattacc
    11941 cgcagcaatg ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa tcatcttgcg
    12001 cgtccaccac agcgcactgc tgatgtagat cgccgcaaca ataatcatgc tgataaacac
    12061 cagcgccgaa atctgatagt tacgctggct ttgcgcactg gcggcttcca gcacatggtt
    12121 gatctccagc tgccaggcct caaagttaac gtcaaacgca ttctgtgatt cctgcaccgg
    12181 cgcagtgagg aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc
    12241 gttgttgtgc cagcgggcaa aacttttctc cgtctctttt tgcaacgccc tgacgtgctc
    12301 gttgcccgca gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc
    12361 gcgcgccgtc gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt
    12421 gcccgctttg ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc
    12481 cgcacgttgc tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc
    12541 ccaaaaagac atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa
    12601 cagcgtggtc gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac
    12661 cgcgttagta aatactcgtc aaaatgtttc ccagtttgga gcttgttcag caattcgcag
    12721 tcgtggctgt gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt
    12781 gagtgggctg gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc
    12841 ttccagcgca gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac
    12901 gcgatccatt tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga
    12961 tgccgatgca atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt
    13021 cattgtttcc ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg agacggagtc
    13081 ttcaatgagg gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac
    13141 ttcacccgcc accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc
    13201 ggcattcagc gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc
    13261 ggcaattttc ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac
    13321 tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc
    13381 gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc
    13441 cagcgcggat gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc
    13501 acgggtaccg gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa
    13561 agagcgttgc atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat
    13621 ggtcagggta ttcgccaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc
    13681 cagcggagta agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac
    13741 caccagcgcg ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt
    13801 gacgatatcg cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat
    13861 tgcattttgc attccctggg ttggctgagc gaaataagct ccagtattgc cataatcaag
    13921 ataatcaatc agttcagtta acgctgtgtg atagttttta tatttttcat caatattacg
    13981 actggtagcg accatttcag gtaacggtgc catgcttttg aattttttat aatgagtcgc
    14041 ggcctgggcc aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg
    14101 attggaggaa tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag
    14161 cattaaatcc caggttgacg tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac
    14221 aaagctcttc tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa
    14281 cagtgcgaat acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat
    14341 aaggcacctt cctgaaaaca agttaatctc gttatcggca agaagggggg aaactttatt
    14401 gctgatgcca cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca
    14461 tttcaattga ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc
    14521 catctcttcg ctgttcagca gtttttcgat gttcaccaga atcaacatcc ggtcgcccag
    14581 tgcgcccagt ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg
    14641 aatttgctcc gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac
    14701 ccgctgtccg agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg
    14761 gctgaactta attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc
    14821 tttgataaac gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg
    14881 cactttcagg atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg
    14941 gcctgacggc tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt
    15001 ttactcattc aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg
    15061 gagacatcaa caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg
    15121 gggactttgc gatagttact ttcaaggttt ttaaccacaa cctggtgctg accaattaat
    15181 tgatccacca gcaaggcgta gcggcgaccg ccactttgta agatcaccac aattccctgg
    15241 gtggcttcgg ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga
    15301 tattcacccc gcacttccag cacccgctcg ccgccggcca gtggatgcag atcggcttca
    15361 cggggttgca gtgattccat aacagcattc agcggcagaa tgaaaacttc atccgcaacg
    15421 cgtacggaca tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg
    15481 ccagtaccct gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta
    15541 acgacgtcca tgccgacgcc gcgcccggag acgtcggtga cctgctctgc cgtggagaag
    15601 cctggtgcaa atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa
    15661 ccttgcgagg ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc
    15721 acttcaatgc aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg
    15781 tttttacctg cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt
    15841 accaggtggg tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa
    15901 ctgcccacca gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc
    15961 cggggatagc gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc
    16021 tgcaaatcac gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta
    16081 accgggtcca gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg
    16141 acgaggttaa ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg
    16201 ctgcgcgtcg ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta
    16261 agcactggtg gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg
    16321 gcttcaatca caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat
    16381 attgccgaga gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt
    16441 tcttcttcca gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc
    16501 gactgactgc gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc
    16561 actgcggatg gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata
    16621 taatcgaagc tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc
    16681 atgatgtcct tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct
    16741 cgtctggctt catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag
    16801 ccaaaagttc ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt
    16861 tcggcatctg gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt
    16921 tcgtccgctt catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca
    16981 cctcggttcg gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc
    17041 cagggcgctt actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc
    17101 ttgtttgttc agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc
    17161 gcttaagcgc atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc
    17221 tccgaccatc agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct
    17281 atatcctttc tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg
    17341 gttgggaata ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc
    17401 gacatcggca ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg
    17461 tagaccttcc tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc
    17521 ggactctatc aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg
    17581 ttttttcagc tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc
    17641 atcaccaccg ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc
    17701 agtcgccagt ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat
    17761 ggagatcagc cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc
    17821 aatcttccac gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat
    17881 aatcggatgc gcttgattct tcatgcttcc tcggttgtca tctgttgttg cggatttttc
    17941 accgcacgca catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt
    18001 ttgcgaccaa actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg
    18061 actttgacgc actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct
    18121 aatggggaaa taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt
    18181 gcgataagcg cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgacc
    18241 cccattacag ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg
    18301 ttcgccggga cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg
    18361 atttcgaagg tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca
    18421 agcatgacgc tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga
    18481 ttttcaatat cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga
    18541 taaagcagag ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc
    18601 aacggcagcg ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac
    18661 ccaatccctg caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt
    18721 ccaccggtca tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag
    18781 ataagcacga catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt
    18841 aacgcgcgat tggggcaaaa aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg
    18901 cctcaccgta tcagttaaac agcctgtact ctctgttcat ccagcagttg tgggataata
    18961 tcggcaggat tctgggaaag tttacgtctt tttactgccc gggatggcgg ttgacataag
    19021 ctgcaggcaa agctgccaac aggctggtga gcgtgggtaa taaaattgcc gccgcagcag
    19081 ttgcagctgg aaagttgcag taatccactt tcaacaaacc gcaccaatgt ccaggcacgg
    19141 gttaatgcca gcagtggtcc ttcttctgct tgtgggcact gttcaaggta taaacggtag
    19201 gctttgatca ccgcatcgac gccattacac aaaccggttt tcagtaaaaa ctgccatgca
    19261 ttacagaaca tcgaagcatg aacgttttgt tcccaggtca taaaccagtc ggttgagaat
    19321 ggcagcatgc ctttcggcgg tgggcttccg cgcagttctt tataaagttt tatcaggcgt
    19381 ccgcgactta actgtgtttc gctttccagc atctgcaaac gagcgcccag ggtgatcaat
    19441 tccattgcca gctgaatatc ccgcgcttcc tgaacaatgc ttttttcact catgatcagg
    19501 cccttttctt gcgcagcgct tcttcaggct gattaacatc attcagcaag cgtgttgaga
    19561 gcatgatgcc ggtatgaatt tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag
    19621 taatcgtctg gtggctgtca aaacggaagt gacaaaccag ttgattggtt tctgccagct
    19681 taaccatttg cggaagagtc agtgccgcta acgttgtcgc catttcttca tttatgccga
    19741 gacgaaacat agcggacgct ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg
    19801 acaagttgat gtcataaatg tgtttcagca actcggaggt atgcattatt cccacccaga
    19861 ataaccaact ttatttttat gcggtttcac cgcaccccgt gatgtcgccg ggaagccccg
    19921 gtaaaaaata attagcatta gaatagttgc gataagctgc aataagcaga accacctttt
    19981 tggtttaata
//
LOCUS       DAZFRJ010000072        19386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000072 DAZFRJ010000000
VERSION     DAZFRJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.072"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKUN_002395"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKUN_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO6660643.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKUN_002396"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKUN_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6660644.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKUN_002397"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKUN_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO6660645.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKUN_002398"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKUN_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO6660646.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKUN_002399"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKUN_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO6660647.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKUN_002400"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKUN_002400"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6660648.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKUN_002401"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKUN_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO6660649.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHKUN_002402"
     CDS             complement(7218..7283)
                     /locus_tag="ACHKUN_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660650.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKUN_002403"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKUN_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO6660651.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="ACHKUN_002404"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="ACHKUN_002404"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO6660652.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKUN_002405"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKUN_002405"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO6660653.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKUN_002406"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKUN_002406"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO6660654.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKUN_002407"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKUN_002407"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO6660655.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKUN_002408"
     CDS             11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKUN_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO6660656.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKUN_002409"
     CDS             complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKUN_002409"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO6660657.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHKUN_002410"
                     /pseudo
     CDS             15415..15660
                     /gene="mcbA"
                     /locus_tag="ACHKUN_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
     gene            15938..18220
                     /gene="fiu"
                     /locus_tag="ACHKUN_002411"
     CDS             15938..18220
                     /gene="fiu"
                     /locus_tag="ACHKUN_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO6660658.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHKUN_002412"
     CDS             18262..18939
                     /gene="ybiX"
                     /locus_tag="ACHKUN_002412"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO6660659.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHKUN_002413"
     CDS             19013..19279
                     /gene="ybiI"
                     /locus_tag="ACHKUN_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO6660660.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYSRRGSKDSQLR"
BASE COUNT         4759 a         5091 c         4785 g         4751 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtacctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattccgt ggtctggaat ttaccataac
     6841 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agagccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccatcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgctctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcataatat cgatttgaac
    11221 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg
    12601 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caatgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggcggag ctgcgtccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    13201 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgtttc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatatcatta
    13681 ccggggtgtt tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc
    13741 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    13801 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    13861 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctgatta
    13981 ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    14101 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcgaccccg gctgaaccgc cgccgccggg ggagccaacg ctttaacgtt
    14221 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    14281 tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca
    14341 gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca
    14401 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    14461 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    14521 ccaggttacg gcgtttacgc tcactacctg cccgcgctgc agcggcggaa tcgtggaatg
    14581 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaattg
    14641 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    14701 taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    14761 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    14821 tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    14881 tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg
    14941 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    15001 caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcctg agttcaggat
    15061 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    15121 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    15181 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    15241 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    15301 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca ttttcatcgc
    15361 ccaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    15421 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    15481 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    15541 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    15601 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    15661 ccatctacaa ataaccccac caacgtaact gcattcgcct ctggatgcag ttttcgcctg
    15721 ttttgtaatc aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc
    15781 gcaatgggtg atagaaaatc gcgccaagtg ataatgctta tcaaaattat tatcacttgc
    15841 acgaacacta tcacgagatt aacagtggca tcgcatccgc agagaggctt tctcgtggca
    15901 gtgaaaattt caacatataa aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc
    15961 tgccagacaa tttcattcgc tcacgttctt tgccggtctt tgtattggca tcacgcctgt
    16021 ggctcaggca ctcgccgccg aagggcaaac taacgcggat gacacgctgg ttgtcgaagc
    16081 atcaacgcct tcgctttatg cgccacaaca atctgccgat ccgaaattct cgcgtccggt
    16141 agcggatact acccgcacga tgacggtgat ttctgaacaa gtgattaaag atcagggcgc
    16201 aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg ggtgcgtttt ttgcgggtga
    16261 gaacggtaac tccaccactg gcgacgccat ttatatgcgc ggtgctgata cctctaacag
    16321 tatttatatt gatggcattc gcgatatcgg cagcgtctcg cgcgacacct tcaatactga
    16381 gcaggtcgaa gtgattaaag ggccttccgg caccgactac gggcgcagcg cgccgacggg
    16441 ctcgatcaat atgatcagca agcagccgcg caatgattcc ggcattgacg cctccgccag
    16501 tattggcagc gcctggttcc gccgcggcac gctggacgtc aatcaggtca ttggtgatac
    16561 cactgcggtg cgcctgaatg tgatgggcga aaaaacgcac gatgccggac gcgacaaagt
    16621 caaaaatgag cgttacggcg tcgccccttc tatcgctttt ggccttggta cagcgaatcg
    16681 tttgtatctt aattatctgc atgtcaccca gcacaacacg ccagacggcg gcattccgac
    16741 catcggtttg ccgggttatt ctgccccatc tgcgggaaca gcggctctga atcattccgg
    16801 aaaagttgat actcataact tttacggcac ggattccgat tacgacgatt cgaccaccga
    16861 caccgccacc atgcgttttg agcacgacat caacgataac accaccattc gcaatactac
    16921 ccgttggtcg cgcgtaaagc aggattacct gatgacggcg attatgggcg gggcgtcgaa
    16981 tattactcag cccaccagcg atgtgaatag ctggacctgg tcacgcacgg cgaataccaa
    17041 agatgtgagt aataaaattc tcaccaacca gaccaacctg acctcgacgt tctataccgg
    17101 ttctatcggt catgatgtca gtaccggcgt ggaatttacc cgtgaaacgc agactaacta
    17161 cggcgttaat ccggtgacgt tacccgcggt aaatatttat catcctgaca gcagcattca
    17221 tcccggcggc ctgacgcgca acggcgcaaa cgccaatggt cagacggata ccttcgcaat
    17281 ttacgccttc gatacgctgc aaatcacccg tgattttgag ctgaacggcg ggatccgtct
    17341 ggataattat catactgaat atgacagtgc caccgcctgc ggcggcagcg gacgcggtgc
    17401 catcacctgc ccaactggtg tggcaaaagg ttctccggtc accaccgtcg acaccgccaa
    17461 gtcgggcaat ctggtgaact ggaaagccgg ggcgctgtat cacctgacgg aaaacggtaa
    17521 tgtctatatt aactatgccg tttcccagca gcctccgggc ggcaacaact tcgcccttgc
    17581 gcagtctggc agcggtaaca gtgccaaccg caccgatttt aaaccacaaa aagccaacac
    17641 cagcgagatt ggcaccaaat ggcaggttct ggataaacgc ctgttgctca ccgccgcgct
    17701 attccgtact gatatcgaaa atgaagttga gcaaaatgat gacggaactt actcgcaata
    17761 cggtaagaaa cgtgtcgaag gctatgagat atccgtggcc gggaatatca cgcccgcgtg
    17821 gcaggtgatt ggcggctata cccagcaaaa agcaaccatc aaaaacggca aagatgttgc
    17881 ccaggatggt tcctcatcgc tgccgtatac cccggagcac gccttcacct tatggagcca
    17941 gtatcaggca acagatgata tctctgttgg cgcgggcgca cgctatatcg gcagtatgca
    18001 taaaggttca gacggcgcgg tgggaacgcc agcgtttacc gaaggttact gggtcgccga
    18061 tgccaaactg gggtatcggg ttaatcgcaa tctcgacttc cagctaaacg tttacaacct
    18121 gtttgatacc gattacgtcg cctcaatcaa caagagcggc taccgttatc acccgggcga
    18181 gccaagaacc ttcttgctca cagccaatat gcatttctga ttcagatgtg gggcgcaggc
    18241 cccacttttt ggagaacttg tatgatgtac cacattcccg gcgtgttatc gccacaggac
    18301 gtcgcccggt ttcgcgaaca actggaacaa gccgaatggg tggatggacg cgtcaccacc
    18361 ggcgcacaag gtgcgcaagt taagaacaat caacaggtcg acacccgcag cacgttatac
    18421 gccgccctgc aaaatgaggt gctgaacgcg gttaaccaac atgctttatt ctttgccgcg
    18481 gccttgccgc gtaccctttc cacgccgctg tttaatcgct atcagaacaa tgaaacctat
    18541 ggtttccatg tggatggcgc agtacgcagc catccacaaa acggctggat gcgtactgac
    18601 ctttctgcca cgctgttttt aagcgatcca caaagctacg acggcggcga actggtcgtt
    18661 aatgacacct tcggacaaca tcgggtaaaa ctcccggcag gcgatctcgt gttgtatccc
    18721 tccagcagcc tgcattgcgt gacacccgta acccgcggcg tacgagtggc atcatttatg
    18781 tggatccagt cgatgatccg tgatgataaa aagcgcgcca tgctgtttga actggacaac
    18841 aatattcagt cgctgaaaag ccgctacggc gaaagtgaag agatcctgtc gctgcttaat
    18901 ctttatcata atctgctgcg ggaatggtcg gagatctgat gcttgaactg cccaggttcg
    18961 tccctggatt agctacactt aactgtacaa gcattgatat ggggaggacg atatggcatc
    19021 cggttgggct aacgatgacg ccgtcaacga acagatcaac agtacaattg aagatgcgat
    19081 tgcccgcgct cggggtgaaa ttccgcgtgg cgaaagcctg gatgaatgtg aagagtgcgg
    19141 tgcccccatc ccgcaagccc gtcgggaagc cattcctggc gtgcgcttat gcattcattg
    19201 tcagcaggag aaaggtttac aaaaaccagc gtatacagga tatagtcgca gaggttcgaa
    19261 agacagccag ttacgttaac tacaactggc gagatgcata gcgagtataa tttgtatttt
    19321 gcgtggcatt ccgtgccttt aacgctgctg tgactgccac gcggcataaa acaagtccat
    19381 aagtac
//
LOCUS       DAZFRJ010000073        19218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000073 DAZFRJ010000000
VERSION     DAZFRJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.073"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(232..879)
                     /gene="ychE"
                     /locus_tag="ACHKUN_002414"
     CDS             complement(232..879)
                     /gene="ychE"
                     /locus_tag="ACHKUN_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO6660661.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(910..963)
                     /gene="ychE"
                     /locus_tag="ACHKUN_002415"
     CDS             complement(910..963)
                     /gene="ychE"
                     /locus_tag="ACHKUN_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO6660662.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1356..4031
                     /gene="adhE"
                     /locus_tag="ACHKUN_002416"
                     /gene_synonym="adhC"
     CDS             1356..4031
                     /gene="adhE"
                     /locus_tag="ACHKUN_002416"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO6660663.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4155..5051
                     /locus_tag="ACHKUN_002417"
                     /pseudo
     CDS             4155..5051
                     /locus_tag="ACHKUN_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5333..5950)
                     /gene="tdk"
                     /locus_tag="ACHKUN_002418"
     CDS             complement(5333..5950)
                     /gene="tdk"
                     /locus_tag="ACHKUN_002418"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO6660664.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6555..6968
                     /gene="hns"
                     /locus_tag="ACHKUN_002419"
     CDS             6555..6968
                     /gene="hns"
                     /locus_tag="ACHKUN_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO6660665.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7112..8020)
                     /gene="galU"
                     /locus_tag="ACHKUN_002420"
     CDS             complement(7112..8020)
                     /gene="galU"
                     /locus_tag="ACHKUN_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO6660666.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8222..9235)
                     /gene="rssB"
                     /locus_tag="ACHKUN_002421"
     CDS             complement(8222..9235)
                     /gene="rssB"
                     /locus_tag="ACHKUN_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO6660667.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9327..10232)
                     /gene="rssA"
                     /locus_tag="ACHKUN_002422"
     CDS             complement(9327..10232)
                     /gene="rssA"
                     /locus_tag="ACHKUN_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO6660668.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10345..10803
                     /gene="ychJ"
                     /locus_tag="ACHKUN_002423"
     CDS             10345..10803
                     /gene="ychJ"
                     /locus_tag="ACHKUN_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO6660669.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10853..11695
                     /gene="purU"
                     /locus_tag="ACHKUN_002424"
     CDS             10853..11695
                     /gene="purU"
                     /locus_tag="ACHKUN_002424"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO6660670.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11855..11939
                     /locus_tag="ACHKUN_002425"
     tRNA            11855..11939
                     /locus_tag="ACHKUN_002425"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11889..11891,aa:Tyr,seq:gta)
     gene            11974..12058
                     /locus_tag="ACHKUN_002426"
     tRNA            11974..12058
                     /locus_tag="ACHKUN_002426"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12008..12010,aa:Tyr,seq:gta)
     gene            12106..12237
                     /locus_tag="ACHKUN_002427"
     ncRNA           12106..12237
                     /ncRNA_class="other"
                     /locus_tag="ACHKUN_002427"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12421..13098)
                     /gene="narI"
                     /locus_tag="ACHKUN_002428"
     CDS             complement(12421..13098)
                     /gene="narI"
                     /locus_tag="ACHKUN_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6660671.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(13098..13808)
                     /gene="narJ"
                     /locus_tag="ACHKUN_002429"
     CDS             complement(13098..13808)
                     /gene="narJ"
                     /locus_tag="ACHKUN_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6660672.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13805..15343)
                     /gene="narH"
                     /locus_tag="ACHKUN_002430"
     CDS             complement(13805..15343)
                     /gene="narH"
                     /locus_tag="ACHKUN_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6660673.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15340..19083)
                     /gene="narG"
                     /locus_tag="ACHKUN_002431"
     CDS             complement(15340..19083)
                     /gene="narG"
                     /locus_tag="ACHKUN_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO6660674.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4850 a         5035 c         4602 g         4731 t
ORIGIN      
        1 gcagtggaat tactaatatc atttcaatta ttcttcagca agcccaggat tatgatgtgg
       61 cgaaaataac atgatggtga ggagaagcag ttaaggaaat ccttataact aactgtaatt
      121 tgagaagaat atttttacct attccttttg cttttgataa ctgcgcggga acaaagtaaa
      181 aataatgacg aagtaaacgg agcatggcag ctccgtttca tttgaaagga attaattaag
      241 caggccgggg aaaatcccct taataccagt aacgataaat tcaatcccca atgccatcag
      301 caatagcccc ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag
      361 ccacggtgcc atgcggaaca atccccagca acataaagcg aacaatgcaa tagccacaaa
      421 gaaaccaaac agatagctaa tgctgtgata acgcgtaccc cagacgatgg tagaactgat
      481 cgcccctggc cccgccatca acggcaacgc caatggcacc acaccaatgc tttcacgtac
      541 cgcggtttct gatttttctt gcttgttctg tttatcctcg ccaagcttgc cgctgatcat
      601 cgacatcgct atcgtcacca ccaggatgcc cccggcgata cggaacgaat caattgatat
      661 accaaaaagt tgcagaatcg tgtcgccgag aaaaagcgag atccacaaga taatggccac
      721 agacaggttg gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat aactggtcat
      781 gctgataaag acgggaataa tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa
      841 tttgaagtaa acgggaaaat caaaaaaggt ctgaatcacg gttagctccg aagcaaaagc
      901 cggataatgt tagccataaa taaggttgaa aagacgcgct gacaatacgc cttttgacag
      961 catttttcac ctcctaacta cttaaaattg ctatcattcg ttattgttat ctagttgtgc
     1021 aaaacatgct aatgtagcca ccaaatcata ctacaattta ttaactgtta gctataatgg
     1081 cgaaaagcga tgctgaaagg tgtcagcttt gcaaaaattt gatttggatc acgtaatcgg
     1141 tacccagaag tgagtaatct tgcttacgcc acctggaagt gacgcattag agataataac
     1201 tctaatgttt aaactctttt agtaaatcac agtgagtgtg agcgcgagta agcttttgat
     1261 tttcataggt taagcaaatc atcaccgcac tgactatact ctcgtattcg agcagatgat
     1321 ttactaaaaa agtttaacat tatcaggaga gcattatggc tgttactaat gtcgctgaac
     1381 ttaacgcact cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt ttcactcaag
     1441 agcaagtaga caaaatcttc cgcgccgccg ctctggctgc tgcagatgct cgaatcccac
     1501 tcgcgaaaat ggccgttgcc gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa
     1561 accactttgc ttctgaatat atctacaacg cctataaaga tgaaaaaacc tgtggtgttc
     1621 tgtctgaaga cgacactttt ggtaccatca ctatcgctga accaatcggt attatttgcg
     1681 gtatcgttcc gaccactaac ccgacttcaa ctgctatctt caaatcgctg atcagtctga
     1741 agacccgtaa cgccattatc ttctccccgc acccgcgtgc aaaagatgcc accaacaaag
     1801 cggctgatat cgttctgcag gctgctatcg ctgccggtgc tccgaaagat ctgatcggct
     1861 ggatcgatca accttctgtt gaactgtcta acgcactgat gcaccaccca gacatcaacc
     1921 tgatcctcgc gactggtggt ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag
     1981 ctatcggtgt aggcgcgggc aacactccag ttgttatcga tgaaactgct gatatcaaac
     2041 gtgcagttgc atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg
     2101 aacagtctgt tgttgttgtt gactctgttt atgacgctgt acgtgaacgt tttgcaaccc
     2161 acggcggcta tctgttgcag ggtaaagagc tgaaagctgt tcaggatgtt atcctgaaaa
     2221 acggtgcgct gaacgcggct atcgttggtc agccagccta taaaattgct gaactggcag
     2281 gcttctctgt accagaaaac accaagattc tgatcggtga agtgaccgtt gttgatgaaa
     2341 gcgaaccgtt cgcacatgaa aaactgtccc cgactctggc aatgtaccgc gctaaagatt
     2401 tcgaagacgc ggtagaaaaa gcagagaaac tggttgctat gggcggtatc ggtcatacct
     2461 cttgcctgta cactgaccag gataaccaac cggctcgcgt ttcttacttc ggtcagaaaa
     2521 tgaaaacggc tcgtatcctg attaacaccc cagcgtctca gggtggtatc ggtgacctgt
     2581 ataacttcaa actcgcacct tccctgactc tgggttgtgg ttcttggggt ggtaactcca
     2641 tctctgaaaa cgttggtccg aaacacctga tcaacaagaa aaccgttgct aagcgagctg
     2701 aaaacatgtt gtggcacaaa cttccgaaat ctatctactt ccgccgtggc tccctgccaa
     2761 tcgcgctgga tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg actgaccgct
     2821 tcctgttcaa caatggttat gctgatcaga tcacttccgt actgaaagca gcaggcgttg
     2881 aaactgaagt cttcttcgaa gtagaagcgg acccgaccct gagcatcgtt cgtaaaggtg
     2941 cagaactggc aaactccttc aaaccagacg tgattatcgc gctgggtggt ggttccccga
     3001 tggacgccgc gaagatcatg tgggttatgt acgaacatcc ggaaactcac ttcgaagagc
     3061 tggcgctgcg ctttatggat atccgtaaac gtatctacaa gttcccgaaa atgggcgtga
     3121 aagcgaaaat gatcgctgtc accaccactt ctggtacagg ttctgaagtc actccgtttg
     3181 cggttgtaac tgacgacgct actggtcaga aatatccgct ggcagactat gcgctgactc
     3241 cggatatggc gattgtcgac gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt
     3301 tcggtggtct ggacgcagta actcacgcca tggaagctta tgtttctgta ctggcatctg
     3361 agttctctga tggtcaggct ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt
     3421 cctaccacga agggtctaaa aatccggtag cgcgtgaacg tgttcacagt gcagcgacta
     3481 tcgcgggtat cgcgtttgcg aacgccttcc tgggtgtatg tcactcaatg gcgcacaaac
     3541 tgggttccca gttccatatt ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta
     3601 ttcgctacaa tgcgaacgac aacccgacca agcagactgc attcagccag tatgaccgtc
     3661 cgcaggctcg ccgtcgttat gctgaaattg ccgaccactt gggtctgagc gcaccgggcg
     3721 accgtactgc tgctaagatc gagaaactgc tggcatggct ggaaacgctg aaggctgaac
     3781 tgggtattcc gaaatctatc cgtgaagctg gcgttcagga agcagacttc ctggcgaacg
     3841 tggataaact gtctgaagat gcattcgatg accagtgcac cggcgctaac ccgcgttacc
     3901 cgctgatctc cgagctgaaa cagattctgc tggataccta ctacggtcgt gattatgtag
     3961 aaggtgaaac tgcagcgaag aaagaagctg ctccggctaa agctgagaaa aaagcgaaaa
     4021 aatccgctta atcagtagcg ctgtctggca acataaacgg ccccttctgg gcaatgccga
     4081 tcagttaagg attagttgac cgatccttaa actgaggcac tataacggct tccacaacag
     4141 ggagccgttt tcttatgcca cttcttaatg atctgctcga tttcagtgac catccgctta
     4201 tgcctccgcc ctctgcacaa ctatttgcag aacaccttcc caccgagtgg atacaacact
     4261 gcctgacgct ttctgctcat gcgaccgttc gccgccgtcg tttaccgggg gacatggtta
     4321 tctggatggt ggtgcaatga gccaattacc gatgttgttc gccgtctgaa cctgagcgcg
     4381 gatggcgaag cggggatgaa cctgctggcc cgcagcgctg tcacccaggc gcgtcagcgc
     4441 gtgggggccg ccccagtgga atggctcttc cgccagaccg cacagacagg ggcgcggaac
     4501 gttacctgaa ggatgactgg cacggcctgc aactttttgc cattgatggc gcacagttca
     4561 ggacacctga taaacccgag ctgcgtgaat attatgggtc tgccaacaca tccactaagc
     4621 ggcagaacgc ctacccggtt atgcgtctgg tggccttaat gaacctggga agtcacattc
     4681 tgctgaatgc cgtgaccgca ccttaccggc agagcgaaac tgtgctggcc cactccatgc
     4741 tcgccaccat cccggataac tctattacgt tgtttgataa acttttttac agcgaagacc
     4801 tgctgctgac gctgaaccag aagggttgta accgccactg gctactgcct gcgtggaaga
     4861 atatcgcatc agaaatgata gagctgggaa acacggcgtc aacagggacc atcccgaaac
     4921 gactggagca tcttcggggt gctctggaag tggtgttcat aacaaaacgc ccccgaccat
     4981 caaggccgag gtcggttaag atatcaaaaa cccgttatcc tgtgaaacat agtgccgctc
     5041 ctcttaagtg aacggcattg cccttctggg gccgtatttt ttgtttaccc aaagcaactt
     5101 ttccataaac cgacagcatt agccttcatc atatttgcga cgatgtataa cgcctaaaca
     5161 cagggatatt gtactttaca ggtcacaagt caacgtcggt gcttaagagc cctgtgaggc
     5221 gtatagcggc gttaaaaaac tgccgagaag ggtatatagc ccggaagaag tgcgtaaaac
     5281 gaactgacag gataaaagtg ccctgctcac cctgtcagta aagaaattct tattaatcgt
     5341 ggcgatgcct ttcctgaata gccgttaatg agccgacttg taacgcctct ttatagtgtt
     5401 tacggcatac agaaacgtat cgttcattac caccaattac cacctgctca ccttcgttat
     5461 aaggtctgcc tgcttgatca agacgcagca ccatgcttgc tttacggcca caaaaacaga
     5521 tggtttttaa ttcaaccagt ttgtcggacc atgccagtaa gtattggctg ccaataaata
     5581 attcacctcg aaaatcggta cgtaaaccat aacaaagtac gggtatatcg agttgatcga
     5641 caacctccga taattcatat acttgttgtc tggttaaaaa ctggcattca tcaaccagta
     5701 cgcaatgaat tgcctgctgt tcatgttccg cacgaatctc atcaaataat gatgaatttt
     5761 ggttaaataa ttttgcaggc gatgacaaac ctatacgcga actgactttc ccggcaccaa
     5821 agcgatcatc aatttctgcc gtatatacga cagtgcgcat gccgcgttcc tggtaattgt
     5881 atgaagattg caacaatgct gtagacttac ccgcattcat tgcggaatag tagaaatata
     5941 gctgtgccat cagccacagg ccctcaatga tatgaaataa agttggctgg agtttatcat
     6001 aattcgtcag tttttcagct caacagtatg cacagaagtg ggtatatgcg ttctccctta
     6061 cgaagccttg cataatcctt ctgagctatc attacaactg cctcgcttgt tataagcggg
     6121 taaatgactg ctggtaacta ttcacaatct ttaacctgtt gcgcaagtaa tagccctctg
     6181 ttgacctcca ggagatagtg caatactaag tccatgctct tattgcgact gttctacttt
     6241 tcatcattcg cttaataggg aattctcgta aacacaacta atacagaaga ctgaaaggtc
     6301 gtcagcctac gataatctcc ccataaaatg tgacatgaat caggaagttt taacctcacg
     6361 tgctgcgaaa tcatcggtgt aaatagggct atatgccgcg tcttttctgg ctaattttat
     6421 gaaaagatat ttattggcgg cacaaaataa agaacaattt tgaattcctt acattcctgg
     6481 ctattgcaca actgaattta aggctctatt attacctcaa caaaccaccc caatataagt
     6541 ttgagattac tacaatgagc gaagcactta aaattctgaa caacatccgt actcttcgtg
     6601 cgcaggcaag agaatgtaca cttgaaacgc tggaagaaat gctggaaaaa ttagaagttg
     6661 ttgttaacga acgtcgcgaa gaagaaagcg cggctgctgc tgaagttgaa gagcgcactc
     6721 gtaaactgca gcaatatcgc gaaatgctga tcgctgacgg tattgacccg aacgaactgc
     6781 tgaatagcct tgcttccgtt aaatctggca ccaaagctaa acgtgctcag cgtccggcaa
     6841 aatatagcta cgttgacgaa aacggcgaaa ctaaaacctg gactggccaa ggccgtactc
     6901 cagctgtaat caaaaaagca atggatgagc aaggtaaatc cctcgacgat ttcctgatca
     6961 agcaataatc ttttgtagat tgcacttgct taaaatcccg ccagcggcgg gattttttat
     7021 tgtccggttt aagacaattt aataagaata agctataaaa aaacggcgtc gattgctcaa
     7081 cgccgtttcg tggataacac cgatacggat gttacttctt aatgcccatc tcttcttcaa
     7141 gccaggcttt aaattccgtg ccaagggtgt tatgacgaat accgtattca acgaaggcct
     7201 gcatgtaacc taatttatta ccgcagtcat ggctcttccc tttcatatga taggcttcca
     7261 ccgtttcttt ttcaatcagc atatcaattg cgtcggtgag ctgaatttca tcaccagctc
     7321 ccggaggggt ttttgccagc aacggccaaa tatccgcgct aagtacgtaa cgacccacaa
     7381 tagcgagatt agacggcgca acatccgctt tcggtttttc taccacacca accatcggta
     7441 cgctttcacc cggcgctaat tcaacgcctt tgcaatccac aacgccatat gcggtcacat
     7501 cagcaaccgg ttcaaccatg atctggctat gacccgtttc atcaaagcgg cggatcatct
     7561 ctgccaggtt atcctgtgac aaatcggatt catattcatc cagaataaca tcaggcaaaa
     7621 taacagctac cggttcatca cccactaccg ggtgagcaca caataccgcg tgtcccaggc
     7681 ctttcgccag accctgacga acttgcataa tagtcacgtg cggtggacaa atagactgca
     7741 cttcatcaag cagttgacgt tttacacgtt tttccagcat tgcttccagt tcaaaactgg
     7801 tatcaaagtg gttttcaata gagtttttag atgagtgtgt aaccagcaca atttcagtaa
     7861 tgccagccgc aatacattca ttcacgacgt attgaattaa tggcttatcg acaagtggca
     7921 gcatctcttt cgggatggct ttcgtcgccg gcaacatcct ggttcctaat cccgcaacgg
     7981 ggataacggc ttttttgact ttcgtattaa tggcagccat ttaaattctc ctggactgtt
     8041 cgtgttttga acgtgttcat atttctgtat cgcatcccag tatatcagca ccccgaaggg
     8101 gctaagttct gaaaaggagg cgagttagca taattaagac aattacttat acgaatggcg
     8161 acgatagtac caccagccga cattagcagg taatgcaaat ttagcccgcg ttatcgtttg
     8221 ctcattctgc agacaacatc aagcgcagtc gaccaccggt tccccatatt tggcattgcc
     8281 aggcatcgca tcgctggctc aattgattca aataagcgtt acctaaagtg cctaacggaa
     8341 caccattact gatttgcacc tggtgttcgc cggtatttaa cgtcgcattc agacccgcag
     8401 aaaccagaat gagatttttc agttcgcgat gataatagcc aactaatagc ggaaactgcc
     8461 ccggcagatt ggcctgacga agtaaatggt ttacctgctt caataacgcg cccaactctg
     8521 gcaaccgttg attttggtgt gcaagctgtt cctgtaataa tccgttaaac aatgcgcgta
     8581 ataacaaggc agcaagtacg ccattatgtc cagctcgggt gacatcaagg caataaaatg
     8641 ccagatcgtt ttccgaaagt gcggcaatat caagcaccag gccgggtttg tccgcggcaa
     8701 ccaattgacg ataattaacc cggcaatggg aaatcacctg ctgaaccggc ggttgtagtt
     8761 cctgtaataa tttcgccgct gcggcagggt tatcaaccat tgcatcccag tcgcgaaaaa
     8821 gcctttcctc ttcctcaacg cgcgaattaa acatgctggg atagagacag gcaaaaacca
     8881 tctcgcgcaa gcgattcaaa tctttaactg gtttcagcaa aacatcttca acgcccagac
     8941 gtaacgcttt ggcaatatct gccatatttt cggtggcaga tatcaccaga accggtgtct
     9001 ggtcacctct gttgcgtata tgttccagca gtttaagccc gttcattcgt ggcatcgcga
     9061 tatcacatat catcaggtct ggagtgaaac ctcccagcaa ctcaagggca tccaccccat
     9121 cagccgccag taccgttgtc gctcccaatg aggaaaacca cgaatccaga agcgagcgaa
     9181 ataccggctc atcttcaaca atgagaatct gttttccgac caatggctgc gtcatgttct
     9241 ctcccctgac tggctttact caatagtggc atgctattgc cacctgcgcc tgtcagaatt
     9301 aacttaagtg tagataaaaa ttctggtcaa atgttggtgc gtaccaacgg caaaagttcg
     9361 tccattttct tttccactgc cagctgtccg gccgcaatgg cagcgtgcgc gcgatggaaa
     9421 tcaagcgtag atatttgcgg gcaaacaggt tgaatcagaa tatcgggcgg atcacctgcc
     9481 atgcggttcc ttttaaggcg gttctccagc acctggatag aagtggtcat aatctctgtt
     9541 gccgttggcg ctgtcaccgc ccgacgtgtc gttatgctgc ctaacctttc tttcagacgc
     9601 gcatgccacg gcagagaatc accattctcg ctattttctt cactgacatt aaaggagagc
     9661 aaatcttgtt gcatcaaatg agcatcgtgc tgcaggtcaa ccgctatcac aatatcagcc
     9721 cccaatgcac gcgtgaggga aataggaatt gggttaacga ctgctccatc aaccagccag
     9781 tagccgttat gtgcaacagg tgccatgagt cctggaatac tgcatgatgc gcgaatagca
     9841 agatggagat cgccttcagt aaaccataat tcacgtcccg tacttaaatt ggtggcaaca
     9901 gccgcaaagc gacgggaaca attttcgatc tctgtttccg gcattatttc gcggtattga
     9961 ttgaagacac gctcgccgcg cagtaaccca ccgcgctgcc aggagagatc catcaggcgt
    10021 aaaacatccc aataactgaa agaggtcacc caatcttcca gcgcagataa tcgatcgcat
    10081 gcataggcag cgcccaccag cgaaccaatt gaacatcctg caacgatatc aatttcaata
    10141 cccacttttt ttagcgcatt aataacgcca atatgcgacc aacctctcgc cgcgccagat
    10201 cccagcgcca gccctatttt tatctttctc attattcccg caagattccc ctggaatgca
    10261 atcgtagcca catcgctact gctcggttaa catagtgcta ctcaggcggt taatacgccg
    10321 ttattgtttc cagggagatt atttgtgtct cagctttgtc cctgtggtag tgctgtcgag
    10381 tatagcctat gttgccaccc ttatgtgtct ggtgaaaagg ttgcacctga tccagaacat
    10441 ctcatgcgtt cgcgttactg cgcttttgtg atgcaagacg cagattattt aataaagacc
    10501 tggcatccct cttgtggagc agcggcgtta cgtgccgaat tgatggccgg atttgcgcat
    10561 actgaatggt tgggattgac cgtatttgaa cattgttggc aggacgccga caatatcgga
    10621 tttgtcagtt ttgttgctcg atttaccgaa ggtggtaaaa caggtgcaat aattgaacgt
    10681 tcgcgatttt tgaaagaaaa cggtcaatgg tactatattg acggtacacg tccgcagttt
    10741 ggtcgcaacg atccctgccc ttgtggttca ggtaaaaaat ttaaaaagtg ctgcggccaa
    10801 taatggttga cggtacggtt tagcaaacac tctcaacaag gtttttccag caatgcattc
    10861 actccaacgt aaagttctgc gtactatttg tccggaccaa aaaggtctga tcgcacgtat
    10921 taccaatatt tgctacaagc acgagttaaa tatcgtacag aacaatgaat ttgttgatca
    10981 ccgtaccggg cgctttttta tgcgcacgga actggaaggg atttttaatg attccaccct
    11041 gctggcggat ctcgatagcg cattgccaga aggctccgtg cgtgagctga atcctgccgg
    11101 tcgtcgccgg atagtgattc tggtcactaa agaagcgcat tgccttggcg atttgttgat
    11161 gaaagccaat tacggcggcc tggatgtcga aatcgcggca gttattggta accacgatac
    11221 tttacgttct ctggttgagc gttttgatat tccgtttgag ctggtaagcc atgaagggtt
    11281 aagccgcaac gagcacgatc aaaagatggc ggacgccatt gatgcttatc aatctgacta
    11341 tgtggtgctg gcgaagtata tgcgggtatt aacgccggaa tttgtggcac gcttcccgaa
    11401 taaaatcatc aatattcacc attcattcct gcctgcgttt atcggcgcac gtccttatca
    11461 ccaggcctat gaacgtggtg tgaagattat tggcgcaacc gctcactatg tgaatgacaa
    11521 tctggacgaa ggcccaatca tcatgcagga cgttattcat gtcgatcata cctacacagc
    11581 tgaagatatg atgcgcgcag gtcgtgacgt cgagaaaaac gtcttaagtc gtgcgctata
    11641 caaagtactg gcacagcgcg tctttgttta cggtaatcgg acgattattc tttaatcgcc
    11701 agcaaaaata actggttacc tttaatccgt tacggatgaa aattacgcaa ccagttcatt
    11761 tttctcaacg taacacttta cagcggcgcg tcatttgata tgatgcgccc cgcttcccga
    11821 taagggagca ggccagtaaa aagcattacc ccgtggtggg gttcccgagc ggccaaaggg
    11881 agcagactgt aaatctgccg tcatcgactt cgaaggttcg aatccttccc ccaccaccat
    11941 ctcaagtatt acctcaaaat ttgaattacc cctggtgggg ttcccgagcg gccaaaggga
    12001 gcagactgta aatctgccgt catcgacttc gaaggttcga atccttcccc caccaccatc
    12061 acttttcaaa agtccgtaaa ctctcaaacg aatccgcaat taaatattct gcccaagcgg
    12121 ggaaggatga gaagcttcga ccaaggttcg acttgagcgc cagcgagaga gcgttgccgc
    12181 aggcaacgac ccgaagggcg aagcgcgcag cgttgagtaa tccttccccc accaccatca
    12241 cttccttaaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
    12301 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
    12361 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgc
    12421 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
    12481 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
    12541 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
    12601 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
    12661 gaacggaatg gtcaacaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
    12721 atccgctccg gtagtggttg cacgcacgcg tgggctgaac agacgacgct tcagcaacag
    12781 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
    12841 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcagca taccgaagaa
    12901 gtgaccaaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
    12961 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
    13021 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
    13081 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
    13141 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
    13201 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
    13261 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
    13321 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
    13381 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
    13441 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
    13501 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
    13561 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
    13621 ctgtactggg cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
    13681 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
    13741 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
    13801 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
    13861 tgaacagatt gaatttggta tctgaaccgt ggcagccatc accaaaggta aagccgcagc
    13921 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
    13981 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
    14041 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacggtttca gcacgtttgt
    14101 aatgacgcat cgccaacata cgtttcagtg cgcgcagtac cggtttggta tcaccggcgg
    14161 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaatgc
    14221 cgttgctacc cagctcaccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
    14281 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca
    14341 ttgccatttt ataaaccggc gactgctgtg cggcttcaat aacgctcagc ggaataccgt
    14401 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacc tccagctgac
    14461 gctggtaaag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
    14521 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
    14581 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
    14641 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
    14701 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
    14761 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
    14821 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
    14881 gtttgtcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
    14941 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
    15001 gcgcggtatg caggttctga tagtcaaaat cgaacggctc gtaataatcg tcgatccccg
    15061 gcagatgcgg gttagcgaag attttaccca gcaacatggc acggttaccc atgcgcggct
    15121 gcagtttgcc gttgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
    15181 cagtcgggaa gccctgaccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
    15241 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
    15301 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
    15361 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
    15421 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
    15481 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
    15541 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
    15601 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gagcaccgtt
    15661 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
    15721 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
    15781 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
    15841 gccattggat ttctggccca tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
    15901 aaccagcagg ctttcaccga aatcacgcat ccattggtga tcctgataca gctgctgacg
    15961 gccagagagc gtacgccacg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
    16021 aacgtgttca tcttcaaggc cagaccaggt cgggctggag atgattttgc gcggctgtgc
    16081 ctgaatatcg cggaagcgaa tcttctcgtc ttctttattc agcgccagat gcgtatggtc
    16141 acgaccggta aactcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
    16201 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
    16261 tttcgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
    16321 ggtgttccag gcaatccctt taccgccatt accgattttc tccatcagcg ggccgataga
    16381 ggtaaagcgt tcgtaagtcg ccggataatc gcgctctacg accataatgt gtggcgcggt
    16441 tttaccaggg atcaggtcgc actcgccttt tttccagtct ttcacatcca gcggctgcgc
    16501 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
    16561 atgaccgacg cacacttcgg agaatttctt cgcgatggct ttgtaaattt cccagtcgct
    16621 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
    16681 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
    16741 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
    16801 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
    16861 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
    16921 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
    16981 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
    17041 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
    17101 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
    17161 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
    17221 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
    17281 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
    17341 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
    17401 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
    17461 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
    17521 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
    17581 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
    17641 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
    17701 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
    17761 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
    17821 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
    17881 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
    17941 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
    18001 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
    18061 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
    18121 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
    18181 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
    18241 gacggcaaca gttttggtcc ctttgtaacg cacttcagta aagaagtgag catccggggt
    18301 acgcgtctgc ggtacgtttg acccccaggc gatgatgtag ctggagttgt accagtcagc
    18361 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaagtcgca
    18421 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
    18481 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
    18541 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccagga
    18601 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
    18661 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
    18721 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
    18781 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
    18841 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
    18901 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
    18961 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
    19021 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
    19081 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
    19141 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaagt gtgatgggga
    19201 tgaaaaataa agtaaatt
//
LOCUS       DAZFRJ010000074        19070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000074 DAZFRJ010000000
VERSION     DAZFRJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.074"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            200..505
                     /gene="gfcA"
                     /locus_tag="ACHKUN_002432"
     CDS             200..505
                     /gene="gfcA"
                     /locus_tag="ACHKUN_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO6660675.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            612..1256
                     /gene="gfcB"
                     /locus_tag="ACHKUN_002433"
     CDS             612..1256
                     /gene="gfcB"
                     /locus_tag="ACHKUN_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO6660676.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1253..1999
                     /gene="gfcC"
                     /locus_tag="ACHKUN_002434"
     CDS             1253..1999
                     /gene="gfcC"
                     /locus_tag="ACHKUN_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6660677.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1999..4095
                     /gene="gfcD"
                     /locus_tag="ACHKUN_002435"
     CDS             1999..4095
                     /gene="gfcD"
                     /locus_tag="ACHKUN_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6660678.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4141..5280
                     /gene="gfcE"
                     /locus_tag="ACHKUN_002436"
     CDS             4141..5280
                     /gene="gfcE"
                     /locus_tag="ACHKUN_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO6660679.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5268..5714
                     /gene="etp"
                     /locus_tag="ACHKUN_002437"
                     /gene_synonym="yccY"
     CDS             5268..5714
                     /gene="etp"
                     /locus_tag="ACHKUN_002437"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO6660680.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5734..7914
                     /gene="etk"
                     /locus_tag="ACHKUN_002438"
     CDS             5734..7914
                     /gene="etk"
                     /locus_tag="ACHKUN_002438"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO6660681.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8029..9327)
                     /gene="appA"
                     /locus_tag="ACHKUN_002439"
     CDS             complement(8029..9327)
                     /gene="appA"
                     /locus_tag="ACHKUN_002439"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO6660682.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9407..9499)
                     /gene="cbdX"
                     /locus_tag="ACHKUN_002440"
     CDS             complement(9407..9499)
                     /gene="cbdX"
                     /locus_tag="ACHKUN_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO6660683.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9512..10648)
                     /gene="appB"
                     /locus_tag="ACHKUN_002441"
     CDS             complement(9512..10648)
                     /gene="appB"
                     /locus_tag="ACHKUN_002441"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6660684.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10660..12205)
                     /gene="appC"
                     /locus_tag="ACHKUN_002442"
                     /pseudo
     CDS             complement(10660..12205)
                     /gene="appC"
                     /locus_tag="ACHKUN_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            complement(12339..13196)
                     /gene="hyaF"
                     /locus_tag="ACHKUN_002443"
     CDS             complement(12339..13196)
                     /gene="hyaF"
                     /locus_tag="ACHKUN_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO6660685.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13193..13591)
                     /gene="hyaE"
                     /locus_tag="ACHKUN_002444"
     CDS             complement(13193..13591)
                     /gene="hyaE"
                     /locus_tag="ACHKUN_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO6660686.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13588..14175)
                     /gene="hyaD"
                     /locus_tag="ACHKUN_002445"
     CDS             complement(13588..14175)
                     /gene="hyaD"
                     /locus_tag="ACHKUN_002445"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO6660687.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(14172..14879)
                     /gene="hyaC"
                     /locus_tag="ACHKUN_002446"
     CDS             complement(14172..14879)
                     /gene="hyaC"
                     /locus_tag="ACHKUN_002446"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO6660688.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14898..16691)
                     /gene="hyaB"
                     /locus_tag="ACHKUN_002447"
     CDS             complement(14898..16691)
                     /gene="hyaB"
                     /locus_tag="ACHKUN_002447"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO6660689.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16688..17806)
                     /gene="hyaA"
                     /locus_tag="ACHKUN_002448"
     CDS             complement(16688..17806)
                     /gene="hyaA"
                     /locus_tag="ACHKUN_002448"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO6660690.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18233..18320
                     /locus_tag="ACHKUN_002449"
     tRNA            18233..18320
                     /locus_tag="ACHKUN_002449"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18267..18269,aa:Ser,seq:tga)
     gene            18403..18807
                     /locus_tag="ACHKUN_002450"
                     /pseudo
     CDS             18403..18807
                     /locus_tag="ACHKUN_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
BASE COUNT         4702 a         5039 c         4917 g         4412 t
ORIGIN      
        1 tttatcttat gctcacaagt cgtatttcca gagggggata gttaacaatc ttatatttcc
       61 ctctgcgcgc ctttcctacc cgcagtatat agtgcaagcg gtatgaggaa agggagattt
      121 cacaaggatg ttatactcaa agtagacaat cctgatctca agcgtacgta ttgtccatgc
      181 gatgaataaa ggaaaagtta tgaaacacaa gctttctgca atccttatgg ccttcatgtt
      241 gacgacgcca gctgcgtttg ctgcccctga agcaacaaac gggaccgagg caacgacggg
      301 tactactggc accacaacga ccactaccgg cgcaaccacg actgctacta ccactggtgg
      361 cgtggctgct ggcgctgtcg gcacggcgac agttgtcggt gtggcaacgg ctgttggtgt
      421 ggcgacgttg gcagttgttg cagcaaatga cagtggcgat ggcggttctc ataacacctc
      481 aaccaccacg tccactacac gctaatactg ctttaacgcg tcgaaattaa agttaaatga
      541 tacccggttg cgatattcgt accgggcttt agagatttgc atttttttaa ttaacctttc
      601 aaggattaaa ggtgcgccct cttattttat cgattttcgc actatttctt gcgggatgta
      661 cgcacagcca gcaaagtatg gtcgatacat ttcgcgccag ccttttcgat aatcaggata
      721 tcaccgtagc ggatcagcag atccaggcgt tgccttattc cacgatgtat ttacgcctta
      781 atgaagggca acgaatcttt gtggtactgg gatatataga acaagaacaa agcaaatggt
      841 tatcccagga taacgccatg ctggttaccc acaatggacg tcttttaaaa accgtcaaac
      901 ttaataataa tctgctggaa gtgacaaatt ccgggcagga ccctctgcgt aacgcgctgg
      961 caataaaaga tggcagccgc tggacgcgcg atattctctg gagtgaagac aaccattttc
     1021 gctctgcgac cctgagttct actttttcct ttgctggatt agagacgctg aatattgcgg
     1081 gtcgcaatgt gctgtgtaat gtctggcagg aagaggtgac ttccacgcgg ccagaaaaac
     1141 agtggcaaaa cacattctgg gtcgattcgg ctactggcca ggttcgtcaa agtcggcaaa
     1201 tgttaggcgc aggggttatt cccgtagaaa tgacgtttct taaacccgca ccatgaataa
     1261 attacagtcg tatttcattg ccagcgtact ttacgtaatg acaccccatg cctttgcgca
     1321 aggaacggtg actatttatc tgcctggcga acaacagaca ctttccgttg gccccatgga
     1381 aaatgttgtc cagttggtga cacaaccgca actgcgcgat cgcctctggt ggcctggtgc
     1441 cttgctgacc gactctgcgg cgaaagctaa agcgcttaaa gactaccaac atgtcatggc
     1501 acagcttgct tcctgggaag cagaagctga cgacgatgtg gcggcaacga ttaaatccgt
     1561 gcgccagcag ttactcaatc tcaatatcac tggtcgctta ccggtcaaac tggaccctga
     1621 ttttgtacga gtggatgaga acagcaatcc cccgttggtc ggggactata cgctctatac
     1681 cgtacagcgt ccggtgacta ttactctgct gggtgcggtt tctggggccg ggcaattgcc
     1741 ctggcaggca ggccgtagcg ttaccgatta tctgcaagat cacccccgcc ttgcaggcgc
     1801 ggacaagaat aacgttatgg tgattacgcc tgagggtgag actgtcgtcg cccctgttgc
     1861 cctgtggaat aaaagacatg tggaaccacc gcctggcagc cagctttggt tgggcttttc
     1921 tgcgcatgta ctgcccgaaa aatatgctga tttaaatgac cagattgtgt ctgttttaac
     1981 gcaacgtgtt cctgactgat gaagaagaat tcttatcttt taagctgcct ggccattgcc
     2041 gtctccagtg cctgtcatgc tgaagtatta acctacccgg atccgctggg ttcgtcgcaa
     2101 tcagactttg gcggcacagg attgttgcag atgccaaatg cgcgcatcgc accggaaggt
     2161 gaattcagcg tcaactaccg ggataacgat caataccggt tctactccac ctccgtggcg
     2221 ctgttcccat ggctggaagg caccattcgt tatacggatg tgcgcacacg caaatatagc
     2281 cagtgggaag atttcagcgg cgatcagtca tacaaagaca aatcattcga ttttaaactt
     2341 cgcctgtggg aagaaggtta ctggctaccg caagtggcgt ttggtaaacg tgatattgct
     2401 ggtacgggtc tgtttgacgg tgagtatctg gtggccagca agcaagcggg gccatttgat
     2461 ttcaccctcg ggatggcatg gggctacgcc ggtaatgcgg gcaatattac caacccgttt
     2521 tgccgggtga gcgataaata ttgtcatcgc gcagagtctc acgatgcggg cgatatcagc
     2581 tttagcgata tctttcgtgg cccggcttcc atctttggcg gcattgagta tcaaacgccg
     2641 tggaatcccc tgcgtctgaa actcgaatac gatggcaaca attaccagaa tgatttcgct
     2701 ggcaaactgc ctcaggcaag ccatttcaac gtcggcgcag tttatcgcgc tgccagctgg
     2761 gcagatctca acctgagtta tgaacgcggt aacacgttga tgtttggctt cacgttacgg
     2821 accaatttca acgatctgcg ccctgccctg cgcgatacgc caaaaccggc atatcaacct
     2881 gcgcctgaat ctgaaggatt gcagtacacc acagtagcga accaacttac cgcgctgaag
     2941 tacaacgcag gttttgaagc accggaaatt cagctgcgcg ataagacgct gtatatgtct
     3001 ggtcaacaat acaaataccg tgattctcgc gaagcggtcg atcgtgccaa ccggattctg
     3061 gtgaataacc tgccgcaagg cgttgagaag attagcgtga cgcaaaagcg cgagcatatg
     3121 gcgatggtga ctaccgaaac cgacgtagcc agcctgcgca aacagctggc tggtacagcg
     3181 cctggtcaat cagagcaact tcaacaacaa cgtgttgaag cagaagatct ttctgccttt
     3241 ggtcggggct accgtattcg tgaagatcgc tttagctact ctttcaaccc aacactttca
     3301 cagtcgctgg gcggcccgga agatttctat atgttccagc tggggctgat gtccagtgcc
     3361 cgctactggt ttaccgacca cctgctgctt gatggcggta ttttcaccaa tatttacaac
     3421 aactacgaca agtttaagtc ttcgctgttg cccgcggact ctaccctgcc ccgcgtgcgc
     3481 acgcatatcc gtgattacgt tcgcaatgac gtttatctca acaacttgca ggcgaactac
     3541 tttgccgact taggcaatgg tttctatggc caggtgtatg gcggttatct ggaaacgatg
     3601 tacgccggtg tcggttccga gctgctttat cgcccgctag atgccagctg ggcgctgggt
     3661 gtggacgtta actacgtgaa gcaacgtgac tgggacaaca tgatgcgttt caccgattat
     3721 tccacgccaa caggtttcgt gacggcttac tggaacccgc cgacgctcaa tggcgtactg
     3781 atgaaactta gcgttgggca atatctggca aaagataaag gcgcaacgat cgacgtcgcc
     3841 aaacgctttg acagcggcgt ggcggtaggg gtatgggcgg caatcagtaa cgtatctaaa
     3901 gatgactacg gcgaaggcgg ctttagtaaa ggtttttata tctcgattcc attcgacttg
     3961 atgaccattg gacctaaccg caaccgcgcg gtggtttcgt ggacaccatt gacgcgtgat
     4021 ggtggacaaa tgctgtcacg caaataccag ctctatccaa tgacggcaga gcgagaagta
     4081 ccggttggac aataacctga cgtaattcat aacattacat tgactgataa atataaacca
     4141 atgaaaaaga acatttttaa gttttcggta ctgacattgg ctgttctgtc tttgacagca
     4201 tgtacccttg ttcctggtca gaatttatcg accagtaaca aggatgtcat tgaactacct
     4261 gacaatcagt atgacctgga caaaatggtc aatatctatc ccgtcacgcc aggattgatt
     4321 gatcagttgc gcgccaaacc gattatgtcg caggctaacc cggagctgga gcagcaaatc
     4381 gcaaactatg aatatcgtat cggcattggg gatgtgttga tggttacggt ctgggatcac
     4441 ccggaactaa ccacccccgc agggcaatac cgtagcgcca gtgataccgg caactgggtt
     4501 aacgccgatg gtgcgatttt ctacccctat attggtcgcc tgaaagtggc cgggaaaaca
     4561 cttacccagg tacgcaacga gattacagcg cgtctggatt cagtcattga aagcccgcaa
     4621 gtcgatgtca gcgtcgcggc gttccgctcg caaaaagcgt acgtcaccgg cgaagtcagc
     4681 aaatcgggtc agcagccgat taccaatatt cctctgacca tcatggatgc gattaacgcc
     4741 gcgggcggtc tgaccgcaga tgcagactgg cgcaacgtgg tgctcaccca gaatggcgta
     4801 aagaccaaag tgaacctcta tgcgctgatg cagcgtggcg acttgcgcca gaacaaactg
     4861 ctgcacccgg gcgatatttt atttattccg cgcaacgatg atctgaaagt cttcgtgatg
     4921 ggtgaagtgg gtaaacagag cacgctgaaa atggaccgca gcggtatgac cctggcggaa
     4981 gcgctgggta acgctgaggg tatgaatcag gatgtcgctg atgcgacggg gatttttgtt
     5041 attcgtgcaa cgcaaaacaa gcagaatggc aaaatcgcca atatttatca gctgaatgcg
     5101 aaggacgcat cggcaatgat actcggaacg gaattccagc ttgaacctta cgacattgtt
     5161 tatgtcacta ccgcgccgct ggctcgttgg aaccgtgtga tttctctgct ggtaccgacg
     5221 atttccgggg tgcatgacct gacagagact tcacggtgga ttcaaacatg gcccaactaa
     5281 aatttaactc aatcctggtg gtttgtaccg gcaacatttg ccgttcgccc attggcgagc
     5341 gcttgctgcg taaacgtctg ccgggcgtaa aagtcaaatc ggcaggtgtt catggtctgg
     5401 taaaacaccc tgcagatgcg acagcggcag atgttgccgc taatcatggc gtatctcttg
     5461 aaggacatgc cggacgtaag ctcactgctg agatggcacg aaattacgat ctgattctgg
     5521 caatggagtc ggaacatatt gctcaggtta ccgcaattgc tcccgaggtc cgcgggaaga
     5581 caatgctgtt tgggcaatgg ctggaacaga aagagatccc ggatccctat cgtaaaagtc
     5641 aggacgcatt tgaacatgtc tacggtatgt tggagcgcgc cagtcaggaa tgggcgaagc
     5701 gcctcagccg gtaatttgag tttataaaat acgatgacaa ctaaaaatat gaatacgcca
     5761 ccaggcagca ctcaggaaaa tgagatcgat ctgcttcgtc tggtcggcga gttatgggat
     5821 caccgtaagt ttattatcag cgtgaccgcg ttattcacgc tgatcgctgt cgcttactcg
     5881 ctgttaagca caccaattta tcaggcagat actctggtcc aggttgagca aaaacagggc
     5941 aacgccattc tcagcggcct gagcgatatg atccctaact catcgcccga gtctgcaccg
     6001 gagatccaac tgctgcaatc gcgcatgatt ctcggtaaaa ccattgctga actgaatctg
     6061 cgcgacatag ttgagcagaa gtattttccg attgtgggtc gcggctgggc gagattaacc
     6121 aaagaaaaac caggtgagct ggcgatcagc tggatgcata ttccacaact gaatggtcag
     6181 gatcagcaac tgacactcac ggttggggaa aacggccact atacactgga aggtgaagag
     6241 ttcaccgtca atggtatggt cggccagcgt ctggaaaaag atggcgttgc gctgactatc
     6301 gcggacatta aggccaaacc aggaacacag tttgtcctga gccagcgtac cgaactggaa
     6361 gcgattaacg cattgcagga aacctttacc gttagcgaac gcagtaaaga aagcgggatg
     6421 ctggaactta ccatgactgg tgatgatccc cagttgatta ctcgtattct gaacagcatc
     6481 gctaacaact atttgcaaca gaatatcgct cgccaggcgg cgcaggattc acaaagcctt
     6541 gaattcttac agcgccagtt acctgaagtg cgcagcgagc tggaccaggc ggaagaaaaa
     6601 ctcaacgttt atcgccagca gcgcgattcg gttgacctta acctggaagc caaagccgtt
     6661 cttgagcaga ttgtgaacgt tgataatcaa ctcaatgagc tgaccttccg cgaggcagag
     6721 atctcccagc tgtataagaa agatcaccca acttatcgtg cgctgctgga aaaacgccag
     6781 acgctggagc aagaacgcaa acgcctgaat aagcgggtat cggcaatgcc ttccacccaa
     6841 caggaagtgt tgcgtttaag tcgtgacgta gaagcgggcc gtgcggtata tctgcaatta
     6901 cttaaccgcc agcaggagtt gagtatttcg aaatccagtg ccattggtaa cgtgcggatt
     6961 atcgacccgg cagtcactca gccgcagcca gtgaaaccga aaaaagcgtt gaatgtggtg
     7021 cttggtttta ttcttggcct gtttatttct gtgggtgccg tgctggcgcg tgcgatgttg
     7081 cgtcgtggtg tagaagcccc ggaacaactg gaagagcacg gcatcagcgt ttatgccact
     7141 atcccaatgt ccgagtggct ggataaacgc acccgtctgc gtaagaaaaa tttattttct
     7201 aatcagcagc gccatcgtac taaaaatatc cccttcctgg cggtggataa cccggcggat
     7261 tctgctgtgg aagccgtacg tgcgctacga accagtctgc atttcgctat gatggagacg
     7321 gagaataaca ttctgatgat caccggtgcg acgccagaca gtggtaaaac gtttgtcagt
     7381 tcaactctgg cagcggtgat cgcccagtcc gatcaaaaag tgttatttat tgatgccgac
     7441 ttacgccgtg gttattcgca taacctgttt accgtgagta atgaacatgg cttgtcggaa
     7501 tatctggcag gtaaagatga gctcaacaaa gtgatccagc attttggcaa aggaggcttt
     7561 gatgtgatta ctcgcggtca ggtgccacct aacccgtctg aactgctgat gcgcgatcgg
     7621 atgcgtcaat tactggaatg ggcgaacgac cattacgatc tggtgattgt cgatacgccg
     7681 ccgatgctgg cggtgagtga tgccgcggtc gtggggcgtt ctgttggcac cagcctgctg
     7741 gttgcgcgtt ttggcttgaa caccgccaaa gaggtgagtt tgtcaatgca gcgtctggaa
     7801 caggcaggcg tcaatattaa aggcgctatc ctcaatggtg tgattaaacg cgccagcacc
     7861 gcttacagtt acggctataa ctattacggt tatagttact ccgagaaaga gtaaaactga
     7921 aagtggccgg atgcggcgtg aacgcctttt ccggtctacg cattagacag ttcttcgttt
     7981 gtcatcagcc tcagagcatt caggtaactg aatgctcttt tttatgcatt acaaactgca
     8041 cgccggtatg cgtgcttcat tcacgatttg cgtaaaacct gccaacgaac acatgccctg
     8101 cgcatttcgc tcttcacatc ctgccagggt cagtttcacc tctccgggcg gcgtatttaa
     8161 tgacagcggc gttttatcac gcatctgctg taaagtctgg aagaccagcg aaacctgaat
     8221 ccactggctg ttatcgctta gccgacgcca gcgttcaaac accagttcac cacctggcgg
     8281 cgtgttatcc ggctgaccgg gaagcgtcca gttgagctcc agtgcgccgc cgagatttgc
     8341 cagattagta tcgtgtccgg cgataaacag cactgaagtg ggtaatgtca caccatacgc
     8401 ctgtttttgc ggtggatggg gcgtcaacgc tgtcttgatc aaatctaata acggggtggc
     8461 gcggctgcgg gcaacctctg gcgtgcgttg tagcaaataa aattgcgcgt tatgcaaact
     8521 tagcaaggtg ttccactggt gtgaatcggt gatccttccc caccccggct ccggcattcc
     8581 ctgtgcttgt tgcaggagaa atatctccgt cagcattgat gcgaggctta ccgcaccggt
     8641 taatgagaca ttgtcggcgc tcaccttgag ttccgatggt aatgcctgcg ttaatgaaca
     8701 gctttcgtcc tgtttctcac gtttaaggca caagtttgat tgcggaaaat taagcacccg
     8761 ttccagttcg cgaaacgccg tttgccgatg cccggtaaag tcagcaattg accctcctgc
     8821 cctgctgagg atcgcgtcag tcacgttcgc attatccagt tggcaaacgc cagtttttag
     8881 aggattaaat aacggatcgg gactggacgt atctgcctgg gtatgtacgg ttattgcaca
     8941 gtcaggtgcc agcccggcgg cgaaggcttc gcctgtttta cgggtacgct cgtcgacatc
     9001 agcaataatc gcgacctgac cagactgcgg gcagcccttt ttcgccagca atccgtcggc
     9061 taccagacgc tggcgttggt aatgtccgag ataggcgatt agctcaccac cgcgcggtgt
     9121 cagccaaccc agttttaccg gccaggttgg ccatgcgtct ggggtgacat cctgcatcag
     9181 ttgcgtggcc ttggttggag cacgcacacc atgacgactg acaatcacca cactttccag
     9241 cttcagctcc ggctcactct gagcgaatgc agattgcggg gttaacggaa tcagaagaga
     9301 taaaaatggg attaagatcg ctttcatcga tatgtttccg cttttcatat ctgacattat
     9361 tgattgcctg atgcgatgct aatgcgccgc atcaggccta cgttcgttaa cttttcagac
     9421 gcgggtccag ccataccaac acaagggtgg agagcgaaca catcaacaaa atgccgacga
     9481 accaaagtaa ataccacatt gttcctgctc cttagtacaa ctcgttttcg ttacggcgga
     9541 gagtttctgt tgtcatgcgt ccccacattt tgtagtagct ccagagagtg tagagcaaca
     9601 caatgggcaa aaatatcagc acgattacca acataatgct cagcgtcagc tgactggaag
     9661 tgctgtccca caacgtcagg ctggagattg gactcacgct tgacggcatg acaaagggga
     9721 acagcgtgat gcctgccgtg aaaatcacgc caaattgcat caatgacgcc attaaaaatc
     9781 cccaacccgg gcgaccacga taaatcgcca tcaccgtcag caatgggcag aagaacccca
     9841 gcagcgggaa gatccacaaa acgggcgatt cgacgaaatt attcatccag gcactaggta
     9901 gcactgccac cagtttcatt aacggattgg aaggaccgtt agcatcctgg gcgagcagta
     9961 caaagccatc aataccgacc cacagccagt aacccgccag caaaaagcac agcatcacca
    10021 acagtgcagc gcgtttggtg gccagctgtg aacgcagatg aatcacacca acagttttca
    10081 gttgtaacca gacgccacct tgcaaaatca ccatcccaag gctgagcaat ccgcacaata
    10141 aagggaatgg cgtcagcagt tgccagaagc tgccgagata ctccacgcgt aattgcggtg
    10201 tgaaggcaaa cggcacgccg agcaacaagt tgccgaaggc gataccgaag actaccggcg
    10261 gcaccagact gccgatgacc agaccggcgt cccacatttt acgccagcgt gcatcggcga
    10321 tttttccgcg ataatcaaag gccagcgggc ggaagaacag tgagcacagc accaggatca
    10381 tcgccacata aaagccggaa aacgccgctg catacactct gggccaggcg gcaaataatg
    10441 ccccaccagc gaggatcaac cagacctggt tgccttccca gtgtgcacca acgctgttta
    10501 tcactatccg gcgttcatca tcattacgcg ccaccagcgg cagcagacag ccgatcccca
    10561 tgtcaaatcc gtcggagatc ataaagacca ccaggatcac gccaatcagc agccaccaga
    10621 tgaagcgcaa tgtttcataa tcaaacatga ttttctcctt tacccctgtt gctgcgtcgg
    10681 ttgttcactc tgcatcgcgc tcggccccag acgggcatat ttctgcatca ggtagacttc
    10741 ggcgattaag aacagggtgt aaagccctac gatcatgatc agtgagaaag ccagttgtcc
    10801 tgtggttaaa gcggagtgcg cggagtatgt cggtaagatg tcctgtatcg cccacggctg
    10861 acgaccaaac tcggtcataa accacccggc ttcaatcgca atccacggca acggcaaact
    10921 ccagagcgcc attttcagca cccagcgatg ctggtcgatt ttgccacgca gcgtctggac
    10981 aagcgcaatc agcatcacca gtagcagcag ggaaccacag cccaccatga tgcggaaact
    11041 ccagaatacc ggcgcaacct gaggtatcgc gccacgcatc gccgcctggt actgtgcggc
    11101 tgtgacatga ttcatatccg gcgcatagcg ggagagcaac atgccgtagc ccaggtcacc
    11161 ttccagttcc cggaatgcct gcaacacatg cggctcacga ttgccttgcg atatttcctg
    11221 cattaacagc caggccatac gtccgcgttg caagcgtggg taggtttcag ccatcaaatt
    11281 cttcagaccc ggcacgggtt tatctaatga gtgagtggcg aggatcccta gcagcgcggg
    11341 aattttgatg gcaaaggcgt tacgctcttg atcctgttcc ggccaggcaa ccacatggaa
    11401 cggtgcaggt gcaggttccg tttgccactc cccttccatc gccgccagtt ttaccggttg
    11461 tacttgcgcg acttcatacg cagaactgtc tccgagttgc agggtaccga taatcgccag
    11521 agtaccgaag acggaaccga tggcaaacga acgtaatgcg acatcgcgct cccgtccgcg
    11581 cagtaaatac caggcgctga tcgccataat aaacatggcc ccggtcacgt agcccgccat
    11641 tacggtgtgc acaaatttca cctggctgac cgggttaaag accagctcgc tgaagctggt
    11701 catctccata cgcagggtgt cgatatcaaa atgcgcaccg gtcgggtatt gcatccaacc
    11761 gttggcattc aatatccaca acgcagagag atttgaaccg aacgccacca gccacgtcac
    11821 cagcaggtgc tggtatttat tcagccgttg ccagccgaag aagaacagcc cgacaaaggt
    11881 ggattcgagg aagaaggcca ttaatgcttc catcgccagt ggtgcgccaa aaatatcgcc
    11941 cacatagttg gaatagaatg accagttagt accaaactga aactccatgg tcaggccggt
    12001 agccacgcca agagcaaaat tgataccgaa gagcttaccc cagaagcgcg tcatatcgcg
    12061 gtagattgtt ttgccggtga ccacgtaaat ggtttccatg atagccagca aaaaaatcag
    12121 ccccagggta aggggtacaa ataaaaagtg atacagcgca ggtcagagca aactgccagc
    12181 gcgataaatc aatgacatcc cacatgcgca ctcctgtagg cgaagagaga aaaataaccc
    12241 gataatggta gatctccctc tttatcctga aactctacga ccagaggtat tcgccgattg
    12301 cgatattcat aagactgaaa gcgatactta ccgtcttttt acgtcggtgc agcttccgcc
    12361 agccactgac atacctcgct aagccgctgc gcagagtcga ccaaatcttc tggcgctgcc
    12421 agcaccactt ccggtattgg gcagatttca taactttcca gtaacgggcc ttttaaggtg
    12481 tccgtacagc gtaaatgcca gacatggcgt aaccccgtgg cgttgatata gctctcgcca
    12541 tagccaatgg tacgaatctg aatatttccc ggcccacaga gacgtgagag aaaaagccga
    12601 tcagcctcgc tgatgggtaa ttgcgtcaga ttaatgctgt gcggctgcgc gtcggggtta
    12661 cgtacatgtg ccagtaactc atgcgccaac ggtaggccat tcatcaggcc atcaatgggc
    12721 ggcggtaaca gcgaatctgt cggcaagaga ttttgcgttg ccgcctgcca caacgccagc
    12781 ggcgcgcagc cagcctccag tttgtcctcc agcaactttt cgccgcgacg tctgcgcacc
    12841 cgccataatc cgcagaagat cgcctcctgt atttcacttt cactgtcgtc agcctgctga
    12901 atgcgcactg acacttcccc ttccccgaga agcgtattga taaagtgacg atcgtcggcg
    12961 ttgagattta acagatcgac tgccagcacc tcgcccaacg gcggttggcg ttcgcttagt
    13021 tgatgttgta actcgtttaa cagcgcaatc acctgcgggc tgtgagcaca ttgctccagg
    13081 gccgccatac tcggttcatc attcacctga caggtgatcg gcagtggatt catgctgaaa
    13141 ctgtcatcgt tcggttgcgt tcctggcccc agcagatgga aaaatgtttc gctcatgagg
    13201 cacgctcctg ctgcggttcg acaagcccgc gcatcaggtt tatcagttcc gcccacgggt
    13261 gaataccatt cagcacgccg cgatagtttc cgccggtaaa cactaaagtg gcaggaaagc
    13321 gaaagacgcc aaagcgatcg ccgatggctt cgctctgctc aaggtcagca atcgccacct
    13381 gccatgtata gtcgggaaac tcgcgcagta attcgccaat cattaccgga ttatcgctga
    13441 cctctggcgt gcgtttcggg tcactgctta ataacagcac gccgtctggc gcttgcgtaa
    13501 gccagtcgtc aagacgggat tcactgactg gcgtccagcc gcgcgccagc attcgttgcc
    13561 acaacgcatc aaatggcgtg tcgttgctca tccctgctcc tcctgtgtca tccggtactg
    13621 gcgcaagcga acgccttcgt aattttccat cgacagacag tcataattga gacagcgcga
    13681 ttcattagcc ggttgcggca caattcccca cgcagccagc tgcgccagcg ccgcctgttc
    13741 cgcagcgggc agttgctccc gtgccagttc gctcaggcta ccgccgtagt cgtcgagcat
    13801 tgcgggttgc agaccgacga gggcaatatg tgctggcaga tgtccgcgga tatccgccag
    13861 cgccaacact tcggagaaac tgttctgatg caggctcatt ttcttcgcgc tgagataagc
    13921 cggaatgcgt tctccggcat aggttcgcag cgttccaggt tccagcccgt agtcaatggc
    13981 atcgagaatc aacagatggc tggcgctttc gacatacccc agcaagttca gtccctgagt
    14041 accgccatcg acaatctcca catactcggg ccagtggtaa tgggcataca gccgttccgc
    14101 cacccgcacg ccgaagcctt catcggccca cagcaggttg cccagcccca tgaccaccac
    14161 gcgttgctcg ctcatgaacg ctccttgtta cttattttgc caaatttgtg gctacggtag
    14221 ccgttgacca tggtggagat caccgtgtcg tcggacatga tgtcttcacg cagcgccatg
    14281 tagacatgac cgatcacaaa cgcgccaatc agccacatcc ccagccgatg ccagctgtga
    14341 atgtccattg agttgccacc catccagtag aaaaattcca ccacataacg gaacggcgca
    14401 aaaatagcgt actggctgtg ttcgctatac agcgcaaaac cagtgatgat cataaagacc
    14461 gacatcagga aatagccgaa catcgccgcc tgggcgatgg gattatggcc tatatcggca
    14521 ctcggacgtt ttgccagaaa cagataccag cggatttcat accacacgcc ctgccaccag
    14581 cttttacgcc ataccggcac gataaacagc tcgcgggagt atcgattgcc aacaaaagcc
    14641 cagtagatcc gcatcagcaa aaccacggta aaaaccatcc cggcgctgaa gtgaattaac
    14701 ctgatgtagc ccatatagaa cagatacgtc gcctcgccgc tgacggaagg tagcggcttg
    14761 ccgataaagt acccggtgac catcaacacc gccatgcata acaccgtcaa ccagtgccag
    14821 atgcggactg gcgcttcaaa gacatagtgg ctgacaacgt tgtcgctttt ctgttgcatg
    14881 atgattctcc ttcgctgtta acgcacctgc acagagatca gctcgctacc gtcgtcgccc
    14941 agcacgtgtg ttgaacaggc gaggcacggg tcaaagctgt gcagagtacg caggatctcc
    15001 agcggttgct cggggatcgc cattttggtg ttcatcagcg ccgcttcata agcgccaatc
    15061 tgccctttgg gatcgcgcgg gctggcgttc caggtggtcg gcaccacgca ctggtagaga
    15121 tcaatcttgc catcgcgaat ggcggcccag tggcctaacg ccccgcgcgg cgcttcggta
    15181 aaaccgacac cacggcactc tgtcggccag gttgcaggtt cccatttttc cgtggaagca
    15241 gtggcgagat tgccgttttt caggttggtc atcagcttgt cgaagaaata ctgcaactta
    15301 cctgcggccc actgcgcttc gtgcgcgcgg cacaaaatgc ggcctaacgt tgactggata
    15361 ccggaaagcg gcaggttcaa cgccgacatc atgcgatcga ccgactcaac ggtcgcagca
    15421 tcgcctttgt gataagcgat taacgtgcgc gccagcggcc ccacttccat cgcgttaccg
    15481 cgccagcgtg gcgctttgat ccacgagtag cgttcctgtt cattcagctg ctgaatgttg
    15541 gtatcgctgc ctttgacatc gccggggttg taccacgggt cggtgatgcc atcgaacgga
    15601 tgacgcccga cctgatcgtt gggatatcga taccaggcgt ggtcgacaaa ctcctgcacc
    15661 tgctgcggat caaccaaatc cactggcagc acattgttga agtcgccgtt aatcaccgcg
    15721 ccgccaggca tcagcagact tttctcgcca aagtcgttgg caatatccgg gaatgcgccg
    15781 tagctgagaa cgcatttatc agaaagacca gtgccgattt cgctccacgg tttgttgaac
    15841 tgaccgatgg ctaaggcgtc ggggatcatc acgttgttaa tgaagtccgc cgtgcgggtg
    15901 ataattgact gcaccaggtt caggcgttcc atattgactg ccccgaccgc gccgctttcg
    15961 tcaatgttga tggcgcaagg catcccgccg acaatccagt ttggatgcgg gtttttaccg
    16021 ccaaagaccg cgtggatttt gacaatttca cgctggaaat cgagagcttc gagatagtgg
    16081 gcaaagccca tcaggttagc ttctggcggc agtttgtact gcgggtgccc ccagtagcca
    16141 ttgcggaaga tccccaactg cccgccttca acaaattttt tcaggcggtt ttgtacgtcg
    16201 aagaaatagc caggggatga tttcggccaa gaggagagac tttgcgccag ttcggaggtt
    16261 ttccgcgggt cggctttcag cgcatctaac acatcgatcc agtccatccc ggcaagctga
    16321 tagaagtgca ccagatgatc gtggcaccag agcgttgcca gcataatgtt gcggatgata
    16381 tttgcgttgt ccggcacttt aataccgata gcatcttcga tggcgtaaac cgaagccagg
    16441 gcgtgtacgc cagtacagac gccgcagata cgttcaacga acgcccacgc atcgcgcggg
    16501 tcgcgccctt gtaggatgat ctccagcccg cgaaacatgg tgccgcagga gacggcattg
    16561 gtgatcacat tctgatcgtt aatattcact tcgcagcgca tgtggccttc gatgcgcgta
    16621 atcgggtcga ccaccaggcg gcgtccggca ttattgatgg tgtatccctg agtttcgtac
    16681 tgagtgctca tgcctgttta tcctcattgc ctggctgatg ttcggtttct gtaggttgct
    16741 ggttatgacg tctgcgctgg tcaacggcgc tggcgactgc gtgcacacca acagccgctg
    16801 ccaccacgcc aagcgcggtt aaaccgacgg tatcggcggt ggaatgagta cccatttgcg
    16861 gaatatcgac cacgcggctg tagaacgaac cgcgatccca gaaaccattt tccgcacagc
    16921 ccaggcagcc gtgaccagac tggattggga aagaaacgcc atcattccag cgtgtggaag
    16981 aacaggcgtt ataggtggta ggccctttgc agcccatttt gtacaggcag taacctttgc
    17041 gggcagcgtc atcatcccaa ctctggacga actctccggc gtcgaagtgg gcgcggcgat
    17101 agcatttatc gtggattcgc tgaccataga acatcagcgg acggcccatt ctgtcgacat
    17161 ctggcaagcg atcaaaggtc accatgtaag taatgatggc gctcatcaca tccgggatcg
    17221 gcgggcagcc aggtactttg ataatgggtt tgtcggtgat gactttgtcg ataggcgttg
    17281 cctgcgtcgg attgggccgc gcggcctgca cgcagcccca ggacgcgcag gttccccagg
    17341 cgataatcgc gctggctccg gcagcggcac gtttgagttt ctcaataaac ggtcgaccgc
    17401 tgctgataca gaacatcccc tgctcgccca gcggcggatt accttctact gcgaggatat
    17461 atttgccatt gtattgcgtg atgatgtctt cgaagacttc ttccgcctgg gttccggcgg
    17521 cagccatcaa agtatcgtca taatcgaggg aaatcaggga aaggatgacg tccttcgcca
    17581 gtgggtgagc ggagcggata aaagattcgg tacagcaggt gcattccaga ccgtggatcc
    17641 ataccaccgg aatgcggggt ttgttctcca gcgcccaggc aatctttggt gccattcccg
    17701 cgcctaatcc cagcgacgtg gcagccagac tacaatattt gagaaagctg cgccgggtaa
    17761 cgccctgacg ccgcatggcc tggtaaaatg tttcctcgtt attcatatcg cacgtctctt
    17821 ctccttgcga caccggcagg acaccgggcg cttatggtcg gcaagcgacg tccctgtagc
    17881 aaggcaaacc atcgcttcac gtgagatgct gaaaacgaaa gctcatcctt ctgcactggc
    17941 acacgtcgcc agaaagtatt gttaataaag cgtagtgaaa cttttgcaca aaacaataca
    18001 aactgtgtgg atttatcttt tagcgataaa aatggaccta tttttctttt ggccgggcgg
    18061 tggggatgtt tagccggttg ctaaacgagt aaaagagaag gaattcatgg tgatgaaatg
    18121 atcaatctgc tgttatttca cgctatcaat gcacttttgg ctgtttttca ggcaaacaaa
    18181 caaattaggg gtttacacgc cgcatcggga tgtttatagt gcgcgtcatt ccggaagtgt
    18241 ggccgagcgg ttgaaggcac cggtcttgaa aaccggcgac ccgaaagggt tccagagttc
    18301 gaatctctgc gcttccgcca aataatcaag gggttaccaa atggtagccc ctttgttttt
    18361 tggctcttgg tataaacttg gtataccaat tggtatattt ttattcggat gagattatcg
    18421 gtcatgagcg gaacctcagg ttcctcgtct gatgccgctc ttgcaacacg ttacgctgcc
    18481 gagtattttt gtaaaacatg gactgctcca ggcctgaacc aggcagaagg atacaaagct
    18541 atctcagatc tgtcacatca ctattttcgt gcagaaggct ctagccctcc ccaatcaatg
    18601 gcgacaggta taaataaggt tcgttgtgat gcaggaatga aagagaaaca tgtaaactca
    18661 ctggatatat ttacacatag ttatccagaa atttatgctg gagtagcatt aacacttgca
    18721 ggagtatgca gtaaagataa acaggcgatg tataatatta cacaagaaac cgtccgtatc
    18781 cttcacgaaa ctgaaacaaa agattgaaca ttctcaataa agagctgcgc caagcagcac
    18841 atattctgca ttgcagtaag atatgcatgc ccagccccct gtaaacctat aaatatttca
    18901 aatcacattg atcatcttat agccatattt tttgtgacac cctgtttcag tgaacatgaa
    18961 gacactcacc ttgttcctga tattcattag catcatcctg attatttaac ttctctattc
    19021 agggaagccc gtctgataca aagcttctat ttctcgttaa atattctcca
//
LOCUS       DAZFRJ010000075        18814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000075 DAZFRJ010000000
VERSION     DAZFRJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.075"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            145..873
                     /locus_tag="ACHKUN_002451"
     CDS             145..873
                     /locus_tag="ACHKUN_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6660691.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            873..5723
                     /gene="ehaG"
                     /locus_tag="ACHKUN_002452"
     CDS             873..5723
                     /gene="ehaG"
                     /locus_tag="ACHKUN_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO6660692.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKTTNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITASTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6092..7747
                     /gene="lldP"
                     /locus_tag="ACHKUN_002453"
     CDS             6092..7747
                     /gene="lldP"
                     /locus_tag="ACHKUN_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6660693.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7747..8523
                     /gene="lldR"
                     /locus_tag="ACHKUN_002454"
     CDS             7747..8523
                     /gene="lldR"
                     /locus_tag="ACHKUN_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO6660694.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8520..9710
                     /gene="lldD"
                     /locus_tag="ACHKUN_002455"
     CDS             8520..9710
                     /gene="lldD"
                     /locus_tag="ACHKUN_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO6660695.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9896..10369
                     /gene="trmL"
                     /locus_tag="ACHKUN_002456"
     CDS             9896..10369
                     /gene="trmL"
                     /locus_tag="ACHKUN_002456"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO6660696.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10422..11243)
                     /gene="cysE"
                     /locus_tag="ACHKUN_002457"
     CDS             complement(10422..11243)
                     /gene="cysE"
                     /locus_tag="ACHKUN_002457"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO6660697.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11323..12342)
                     /gene="gpsA"
                     /locus_tag="ACHKUN_002458"
     CDS             complement(11323..12342)
                     /gene="gpsA"
                     /locus_tag="ACHKUN_002458"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO6660698.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12342..12809)
                     /gene="secB"
                     /locus_tag="ACHKUN_002459"
     CDS             complement(12342..12809)
                     /gene="secB"
                     /locus_tag="ACHKUN_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO6660699.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12872..13123)
                     /gene="grxC"
                     /locus_tag="ACHKUN_002460"
     CDS             complement(12872..13123)
                     /gene="grxC"
                     /locus_tag="ACHKUN_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO6660700.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13265..13696)
                     /locus_tag="ACHKUN_002461"
     CDS             complement(13265..13696)
                     /locus_tag="ACHKUN_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO6660701.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13941..15485
                     /gene="gpmM"
                     /locus_tag="ACHKUN_002462"
                     /gene_synonym="pgmI"
     CDS             13941..15485
                     /gene="gpmM"
                     /locus_tag="ACHKUN_002462"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO6660702.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15519..16778
                     /gene="envC"
                     /locus_tag="ACHKUN_002463"
     CDS             15519..16778
                     /gene="envC"
                     /locus_tag="ACHKUN_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO6660703.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16782..17741
                     /locus_tag="ACHKUN_002464"
     CDS             16782..17741
                     /locus_tag="ACHKUN_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO6660704.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17728..18762)
                     /gene="waaH"
                     /locus_tag="ACHKUN_002465"
     CDS             complement(17728..18762)
                     /gene="waaH"
                     /locus_tag="ACHKUN_002465"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO6660705.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         4741 a         4854 c         4871 g         4348 t
ORIGIN      
        1 gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc actgtcatta
       61 atgtaaagca acatagatca ccaataatac aatggacaac tctaccggca ttgatcgctg
      121 gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag tgctgtcact
      181 gtttcagttc tctgcgcatt aacgggatgt gactacattg aagaaggcaa gccagaaagt
      241 agtttattga aacaacaaga agaacataac aataaaattg atctacttga aaaacaacaa
      301 gcacagctaa agagtcaact ggaaactatt caaaaacaac aaactggcat aataagcagt
      361 acaaagactt taactcacgt aattaaatcc gttaaagatc aacaaaacac ttttattttt
      421 accgagttta acccagcaaa aacgaagtat ttcatcctga acaatggttc ggtggcttta
      481 gcgggtcgag tgttatccat tgacgccaca gagaatggca gtgttattca tatttcactg
      541 gtcaacttat taagtacacc tatctcaaat attggtttta atgcgacatg gggtggtgaa
      601 aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa cacatcaatg
      661 aaatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac cctgaagggt
      721 gtatcgccca ataacctggg atatctgaag ttagccatca acatggaaaa tattcagttc
      781 gacaatcttc cttctgctga aaatcggcag aaaagaagca aaaaaataaa tgcagttagt
      841 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
      901 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
      961 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1021 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1081 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1141 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1201 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1261 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1321 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1381 ccaaagcgga aggagcaaac gccattgccc tgggaaatgc cactaaagct actgagatta
     1441 tgagtattgc tcttggcgac accgccaatg cgtcaaaagc gtattcaatg gcgctgggag
     1501 caagtagcgt cgcatctgaa gaaaacgcaa ttgccctggg gcgtagcagt gtagctagcg
     1561 gtactgacag cctcgcattt ggcagacaat cacttgccag cgcagcgaac gctattgcga
     1621 taggtgctga gaccgaagcc gctgaaaatg caactgctat tggcaataat gcgaaggcaa
     1681 aagggactaa tagcatggca atggggttcg gaagccttgc cgataaagtc aatactatcg
     1741 cattaggaaa tggcagccag gctctggcag ataatgcaat cgccataggc cagggcaaca
     1801 aagctgatgg cgtggatgcc atcgctctgg gtaatggtag ccagtcgaga ggcttaaaca
     1861 ccattgcctt aggcacagcc agtaatgcaa ctggtgataa gagtcttgcg cttggtagta
     1921 atagcagtgc caacggtatt aactctgtcg cgctgggcgc agattccatt gcggatttag
     1981 acaataccgt ctctgtcggc aatagttcat taaaacgcaa gatcgttaat gtgaaaaatg
     2041 gcgcgatcaa gtctgacagt tacgatgcca ttaatggttc acagctttat gccattagcg
     2101 actcggtagc aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta
     2161 cagcaccaac ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg
     2221 tacttgatga aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg
     2281 gtactagtag cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct
     2341 ccagtaagga tgcggttaac ggttcccaac tgaaaactac caatgacgat gtcgaagcca
     2401 acaccgccaa tatcgctact aataccagca acattgccac gaatacggca aatattgcca
     2461 ccaataccac caatatcacc aacctgacgg attccgttgg tgaccttcag gctgatgccc
     2521 tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat accaccagca
     2581 aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct gttaacggct
     2641 ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc gccaataaca
     2701 cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg gtgactaatc
     2761 ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca gctcatggca
     2821 ccgagaccac cagcaaaatc accaacgtta aagatggcga cctgacgact ggcagcaccg
     2881 atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg aataccacca
     2941 atatcgccac taacaccacc aacatctcta atctgactga gacggtgact aatcttggtg
     3001 aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat ggcaacaata
     3061 ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt tccgatgcca
     3121 ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc ggcggcaatg
     3181 cttctgtgaa tactgacggt gtgtttaccg gtccaaccta caaaatcggt gaaacaaatt
     3241 attataacgt cggcgatgca ctggctgcga ttaactcctc atttagcacg tctctcggcg
     3301 atgctctgct ttgggatgcc accgcaggta aattcagtgc caaacacggt actaatggtg
     3361 acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc agactccagt tctgacgcag
     3421 taaacggctc acaactccac ggcgtgagca gttatgttgt tgatgcgctg gggggtggtg
     3481 ccgaagtcaa tgcagacggc accatcactg cgccgacgta caccattgct aatgctgatt
     3541 acgataatgt cggtgatgcc ctgaatgcta tcgataccac tcttgacgac gctctgctct
     3601 gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa gataaaactg
     3661 ccagtgtaat cactaacgtc gctaacggtg caatctctgc tgccagcagc gacgcgatta
     3721 acggctcaca actctatacc accaataagt acatcgctga tgcgctgggt ggtgacgcag
     3781 aagtcaacgc tgacggcacc atcaccgcat cgacttacac cattgcgaac gccgagtaca
     3841 acaacgtcgg tgacgccctg gatgcgcttg atgataacgc cttgctgtgg gatgagactg
     3901 ccaatggcgg tgctggagcc tacaatgcca gccatgacgg taaagccagc atcatcacta
     3961 atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc agtgaacggt tctcagttga
     4021 atgcgacgaa tatgatgatt gagcagaaca cccaaattat caatcagctc gctggtaaca
     4081 ccgacgcaac ctatatccaa gaaaacggtg cgggtattaa ctatgtgcgt actaacgacg
     4141 acggcttagc gttcaacgac gccagcgcac agggtgttgg cgctacagct ataggttata
     4201 actctgtcgc caaaggcgat agcagcgtag ctattggtca gggcagctac agcgacgttg
     4261 atacgggtat cgccctgggt agcagctctg tttccagccg agtgattgcc aaaggctccc
     4321 gtgacaccag cataacggaa aatggcgttg ttattggtta cgacaccacg gatggcgaac
     4381 tgctcggtgc attgtctatc ggtgatgacg gtaaatatcg tcaaatcatc aacgtagccg
     4441 atggttccga agcccatgac gccgttacgg ttcgtcaatt gcagaatgcg attggtgcgg
     4501 tcgcaaccac gccgactaaa tacttccacg ctaattcaac ggaagaagat tcactggcag
     4561 tgggaactga ctcgctggca atgggtgcga aaaccatcgt gaatggcgat aaaggtattg
     4621 gtatcggtta tggtgcctac gtggacgcga atgcacttaa cggcattgcc attggtagca
     4681 atgcgcaagt cattcatgtc aacagtattg cgataggtaa tggttctacg accactcgtg
     4741 gcgctcaaac caattatacc gcctacaaca tggacgcacc gcagaactct gtcggtgaat
     4801 tctcagtcgg tagtgcggat ggtcaacgtc agatcactaa cgtcgcagca ggttcggctg
     4861 ataccgatgc ggtcaacgtg ggtcagttga aagtaacgga tgcgcaggtt tcccagaata
     4921 cccagagcat tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata
     4981 tcgaaaacgg tattggcgac attgtcacct ccggtagcac caagtacttc aagaccaaca
     5041 ccgatggcgt agatgccaac gcccagggta aagatagcgt tgctattggt tctggttcca
     5101 ttgctgccgc tgacaacagc gtcgcactgg gtaccggttc cgttgcagag gaagaaaata
     5161 caatctctgt aggttcttct actaaccaac gccgtatcac caacgtagct gccggtgtta
     5221 atgccaccga tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc ggcgtgcgct
     5281 acgacaccaa agctgatggt tctatcgact atagcaatat caccctcggt ggtggcaacg
     5341 gcagtacgac tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact
     5401 acgcgcagtt gaagcaaagc gtgcaggaaa cgaagcaata caccgatcag cggatggttg
     5461 agatggataa caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa
     5521 tggcaatgac cggtctgccg caggcttata caccgggtgc cagcatggct tctattggtg
     5581 gcggtactta caacggtgaa tcggcagttg ctttaggtgt atcgatggtg agcgccaatg
     5641 gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac
     5701 tcggtgccgg tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca
     5761 aattgcctga tgcgttacgc gcatcaggca agataaggcg ctccgggtca acaatctaag
     5821 ggcaattctc tgatgaggat tgcccttttc tttaccagac atctcccccc tacaagaatt
     5881 ggccctacca attcttcgct tatctgacct ctggttcaca attccccaat taaaactcac
     5941 atcaatattg ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac
     6001 acaattggca gtgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta
     6061 cacgatgtgc gtggactcca ggagacctgc aatgaatctc tggcaacaaa actacgatcc
     6121 cgccgggaat atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt
     6181 tgcgctgatt aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg tagcaatcgc
     6241 ccttgccgtg gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcggtggt
     6301 ttatggcttc ttctatggtt tgtggcccat cgcgtggatc attattgcag cagtgttcgt
     6361 ctataagatc tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat
     6421 aacccctgac cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg ctttccttga
     6481 aggagccgca ggctttggcg caccggtagc aattaccgcc gcattgctgg tcggcctggg
     6541 ttttaaaccg ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc cagtggcatt
     6601 tggcgcgatg ggcattccaa tcctggttgc cggacaagta acaggcatcg acagttttga
     6661 gattggccag atggtggggc gtcagctacc gtttatgacc attatcgtgc tgttctggat
     6721 catggcgatt atggacggct ggcgcggtat caaagagacg tggcctgcgg tcgtggttgc
     6781 gggcggctcg tttgccatcg ctcagtacct tagctccaac ttcattgggc cggagctgcc
     6841 ggacattatc tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca
     6901 accagttcgc gtattccgct tcggtgactt gggggcgtca caggttgata tgacgctggc
     6961 ccacaccggt tacaccgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac
     7021 cgctaccgta acactgtgga gcatcccgcc gtttaaagcc ctgttcgcat cgggtggtgc
     7081 gctgtatgag tgggtgatca acattccggt gccgtacctc gataaactgg ttgcccgtat
     7141 gccaccagtg gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc
     7201 tgccaccggc accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa
     7261 accgtctgac gctatcagca ccttcggcag cacgctgaaa gaactggctc tacccatcta
     7321 ctccatcggc atggtgctgg cgttcgcctt tatctcgaac tattccggac tgtcatcaac
     7381 actggcactg gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg
     7441 ctggctgggg gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct
     7501 gcaagccacc gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac
     7561 caccggtggc gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc
     7621 ggtaggcctg gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat
     7681 cttcacctgt atggtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat
     7741 tccttaatga ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg
     7801 ctgattgatg aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg
     7861 atgcaactcg gcgtatcacg taattcacta cgcgaggcgc tagcaaaact ggtgagcgaa
     7921 ggcgtgctgc tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg
     7981 tcggagcaaa acatcgtcca gccgctaaaa acgctgatgg ccgatgatcc ggattacagt
     8041 ttcgatattc tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg
     8101 cgcgccacac ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa
     8161 gacccggata tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca
     8221 cataacatcg tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg
     8281 aagcatagcc gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa
     8341 catcaggctg tcattgacgc catttttgct ggtgatgccg acggggcgcg taaagcaatg
     8401 atggcgcacc ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc
     8461 cacgcacgga ttacccgcct gcccggtgag cataatgagc attcgaggga gaaaaacgca
     8521 tgattatttc cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc
     8581 tgttccacta tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag
     8641 atttgtcaga agtggcactg cgccagcgta ttctgaaaaa catgtccgac ttaagcctgg
     8701 aaacgacgct gtttaatgag aaattgtcga tgccggtagc actggcaccg gtgggtttgt
     8761 gtggcatgta tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggca
     8821 ttccgtttac tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca
     8881 agcgcccaat gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc
     8941 tggagcgagc aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac
     9001 cgggcgcacg ctaccgtgat gcgcattcag gtatgagcgg cccaaatgcg gcaatgcgcc
     9061 gctacttgca ggcggtgacg catccgcaat gggcgtggga tgtgggcctg aacggtcgtc
     9121 cacatgattt aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca
     9181 tcggctggct ggggaataac ttcgatccgt ccatctcatg gaaagacctt gagtggatcc
     9241 gcgatttctg ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg
     9301 atgcagtacg ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg
     9361 acggtgtact ctcttccgct cgtgcgttgc ccgctattgc agatgcggtg aaaggtgata
     9421 tcgccattct ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc
     9481 tcggtgccga caccgtactg ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc
     9541 aggcgggtgt agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg gcgatgacgc
     9601 tgactggcgc gaaatcgatc agtgaaatta cgcaagattc gctggtgcag gggctgggta
     9661 aagagttgcc tacggcactg gctccgatgg cgaaagggaa tgcggcatag tcgtttgcgc
     9721 cccctcaccc taaccctctc cctcagggag aggggaccgt tcggcgctgt atgtactccc
     9781 tcactctgaa acgacaccgc actctttttt tctccctcgc ccctccgggg tgaggggaaa
     9841 aggccgcact gctcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
     9901 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaatatca tccgtctttg
     9961 cgctaatacc ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct gggacgataa
    10021 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
    10081 ctttcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ctacgaaagg
    10141 tacgcctgct cacagcgccg taaactatca ggatggcgat tatctgatgt tcggcccgga
    10201 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
    10261 tccaatggtg ccggacagcc gcagcatgaa cctgtccaat gcggtgtcgg tagtggtgta
    10321 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
    10381 cggatagatg attacatcgc atccggcacg atcacagcac atcagatccc atccccatac
    10441 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
    10501 tctggtttac cgacaatacg agccggaaca ccagcggcgg tagtatgcgg cggcaccggt
    10561 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgaga
    10621 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
    10681 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
    10741 gccgtttcac caacgacgat gcccgtggcg tggtcgagca tgataccgcg accaattttt
    10801 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
    10861 agtgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
    10921 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
    10981 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
    11041 tcaaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
    11101 atgtagctca gggcactgcc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
    11161 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
    11221 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
    11281 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc
    11341 gtccttgcgt gcacgaccta gtaaagtcaa tgctgcctcg cgcgcgtttt ttccgcaata
    11401 taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc
    11461 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
    11521 ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc
    11581 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
    11641 acccagcgcc gcaccaagac gcgacatttc agccagccca cgggtgatca gcgccgtacg
    11701 cgcattcgca ccaaaaccga taccgtcaga catccccgcg ccaatggcaa tgacgttttt
    11761 caccgcgccg ccaagctgca cgccaatgaa atccgggttg ctgtaaacgc ggaaactttt
    11821 gccacagtgc agcaattgtt ggagatcatc ggcaaaggtc tggtcggtag aggccaacga
    11881 aatagccgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
    11941 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
    12001 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
    12061 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacggctggc
    12121 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
    12181 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
    12241 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
    12301 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
    12361 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc
    12421 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga
    12481 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
    12541 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
    12601 tcgcccaggg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
    12661 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
    12721 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
    12781 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
    12841 tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac
    12901 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
    12961 ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
    13021 cgatcggcaa ctcctgaaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
    13081 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
    13141 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
    13201 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
    13261 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
    13321 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
    13381 gcatgccaga accgtctacc acgataaccg gtttgtcttt gtgcttctca agctcaccaa
    13441 cattgttggc tttgatttca ctcggcaaca gattgataga acctgcgata tggcctttac
    13501 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
    13561 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
    13621 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
    13681 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
    13741 ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc
    13801 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
    13861 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
    13921 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
    13981 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
    14041 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
    14101 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
    14161 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
    14221 ttgctaatcc agtgctgact ggcgcggtag ataaagcgaa aaacgcaggt aaagcagtac
    14281 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
    14341 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    14401 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    14461 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    14521 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    14581 tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    14641 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    14701 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    14761 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    14821 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    14881 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    14941 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    15001 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    15061 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    15121 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    15181 cggttaaagc ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    15241 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    15301 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttatgttg
    15361 gtgataagaa cgtgaaagcg gttgaaggcg gcaaactttc tgacatcgcg ccgaccatgt
    15421 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    15481 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    15541 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    15601 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    15661 ccgcaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    15721 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    15781 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    15841 agcaaaaagc cgcccaggag cgcagcctcg ccgcgcaact ggatgccgca ttccgtcagg
    15901 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    15961 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    16021 aaacgcgtga agaagtcgcc atgcagcgtg ctgaactgga agagaaacaa agcgagcaac
    16081 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgagc
    16141 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    16201 aactgcgtgc caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa gccgcggcga
    16261 aagcgcgtgc tgaacgagaa gcgcgtgagg cccaggcggt tcgcgaccgc cagaaagaag
    16321 cgacgcgcaa aggcaccacc tataaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    16381 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc
    16441 atcgctatgg tgaacagcta cagggcgaac tacgctggaa aggaatggtt atcggtgcct
    16501 ctgaaggtac tgaagttaaa gcgattgccg acggtcgggt gattctggct gactggctgc
    16561 aaggttacgg tctggtggtg gtggttgagc acggtaaagg cgacatgagt ctttacggct
    16621 ataatcagag cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccaattgcac
    16681 tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg
    16741 gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    16801 acgttcttgc atttgccgct ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg
    16861 ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc
    16921 cctccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca
    16981 aagcgcataa cagcgggcac gaagtgttga ttcatctccc gatggcacca ctgagtaaac
    17041 agccgctgga gaaaaatacg ctgcgcccgg agatgagcag cgacgaaatt gagcgcatta
    17101 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac atgggtagca
    17161 agatgacctc taacctgttt ggtatgcaga aagtgatgcg ggcactggag cgttacaatc
    17221 tttacttcct cgacagcgta accatcggta atacccaggc gatgcgcgcc gcgcaaggta
    17281 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca
    17341 tccgtgtgca atttaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca
    17401 ttgggcatcc tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc
    17461 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacttcta
    17521 caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt
    17581 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    17641 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    17701 aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg
    17761 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    17821 gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac cggaagtaaa
    17881 tatctcggca atcatccgtt ggcgggtaag aatatccggc tctttgcgca ccgcatggca
    17941 aacacgcaat gcttcgtaag ttatttgctg atgaaattcc ggataaatcg taattttgtc
    18001 ggcataattt cgatttaatt tctccagcag gcgggtaatc ttaatatagt gacgttgata
    18061 attaaggttt ttattccctt gtctatgcaa ccgactcact gacgtattat gcagataata
    18121 tttatataat gattgctcgg tatatcgcgc acgcagcgcg ttaaacatga attctgttgt
    18181 ccagacaata tcctgatgat gtaatccggc aataaattta atgttatttt taacaataac
    18241 atcacggcga taaaccccca tccagacaac gtgagtccaa cggcgcgaag aaagccccat
    18301 ccgcagccag tccgggccgg ttaatacgcc ggttgagcga aggcgatcgc tggggatgga
    18361 ttgccaggtt tctcccgttt cacgaaaaca ccagtcagcg ttgcactgcg ccacgtcgag
    18421 gtcatcctct aacgccatgg tcatcagcgt ttcgtacatg gtgggataga cctcatcgtc
    18481 agcatcgaca aaagcgacat atttgcccgt cgccacctcg atcccacgat tacgcgctac
    18541 cgatgcgcca gcattcgcct gatgcaacaa acgaacgtgc ggatagtttt ctgcgtaatg
    18601 ctttgctatt tcaacagaat tatccgttga accatcgtta ataataatga tttccagagc
    18661 agtccaggtt tgcgtaatta aagattccat acaagtgcgg aaatcatcgc ccgcattata
    18721 taacggaata ataacactaa gtttattggt gctgttcatc ataaattacc gacgacctct
    18781 gaagttatcc tgacgtttta caggctttgt atta
//
LOCUS       DAZFRJ010000076        18638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000076 DAZFRJ010000000
VERSION     DAZFRJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.076"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHKUN_002466"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHKUN_002466"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO6660706.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /locus_tag="ACHKUN_002467"
     CDS             707..1135
                     /locus_tag="ACHKUN_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO6660707.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKUN_002468"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHKUN_002468"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO6660708.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKUN_002469"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHKUN_002469"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO6660709.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKUN_002470"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHKUN_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO6660710.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKUN_002471"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHKUN_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO6660711.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKUN_002472"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHKUN_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO6660712.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKUN_002473"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHKUN_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO6660713.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKUN_002474"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHKUN_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO6660714.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHKUN_002475"
     CDS             complement(8154..9338)
                     /locus_tag="ACHKUN_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660715.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKUN_002476"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHKUN_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO6660716.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKUN_002477"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHKUN_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO6660717.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKUN_002478"
     CDS             complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHKUN_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO6660718.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKUN_002479"
     CDS             complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHKUN_002479"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6660719.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKUN_002480"
     CDS             complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHKUN_002480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6660720.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKUN_002481"
     CDS             complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHKUN_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO6660721.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKUN_002482"
     CDS             complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHKUN_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO6660722.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17180..17509)
                     /locus_tag="ACHKUN_002483"
     CDS             complement(17180..17509)
                     /locus_tag="ACHKUN_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO6660723.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17661..18005
                     /locus_tag="ACHKUN_002484"
     CDS             17661..18005
                     /locus_tag="ACHKUN_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO6660724.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKUN_002485"
     CDS             complement(18042..18491)
                     /gene="alaE"
                     /locus_tag="ACHKUN_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO6660725.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4669 a         4728 c         4826 g         4415 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa taccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagagt gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agcagcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgcca gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tttgaacgct
    12781 cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt aattgccatg
    12841 caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa atagtcactt
    12901 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
    12961 attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc aaatcgagaa
    13021 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
    13081 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
    13141 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
    13201 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
    13261 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
    13321 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
    13381 gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
    13441 tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
    13501 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
    13561 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc ggtattggtc
    13621 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
    13681 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
    13741 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
    13801 acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
    13861 tccataaagc tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc
    13921 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
    13981 cgcattgtca gttgttgttc tacgacactt aatgtctgcc atgcaggtat atcgttcgac
    14041 agtggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
    14101 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
    14161 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
    14221 aggcgaatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
    14281 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacaaacc ctgatcgaca
    14341 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccgatttc gtaagcatcc
    14401 tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
    14461 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
    14521 ttgatataag agatagaacc ggttggcggc accgcctgga gattctggtt gtatatgccg
    14581 tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaataccg
    14641 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
    14701 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
    14761 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
    14821 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
    14881 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
    14941 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg tacggcagtc
    15001 tctaccgtgc gggcaaagtt gggggaatcc atggtgtggg caatattcaa cgaacctaaa
    15061 ttgcaggaaa tatcatggcc tgtacgggca tagtcgagat tctcgtcata ctctgaggcg
    15121 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
    15181 ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
    15241 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
    15301 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
    15361 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
    15421 gtgatatccg ggatcaccac gccaagcgac agtgttttaa tgcggatttt ttcgtcggca
    15481 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
    15541 gcaccagccc cctgacgcgc gccgagttgg ttgggatagg aaaatgcgtc ttccagcatt
    15601 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
    15661 tcccgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag ttgcagtgcg
    15721 gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag aaaacaggaa
    15781 accagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
    15841 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc ttgtgccagc
    15901 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
    15961 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
    16021 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
    16081 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
    16141 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
    16201 ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg
    16261 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
    16321 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
    16381 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
    16441 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
    16501 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
    16561 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
    16621 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
    16681 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
    16741 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
    16801 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
    16861 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac gagtgtaaat
    16921 agtaatgcgc atggttcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc
    16981 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaaccacac
    17041 aatatatagc aaatagtggt cgaaattacc ctggatatga gcgtgacggg gtagggggat
    17101 ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg cttcggggtc aaagacgcgt
    17161 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
    17221 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgcgc
    17281 agccaacggc atcgcgtgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
    17341 tttctttcag caatgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
    17401 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
    17461 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
    17521 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
    17581 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
    17641 gataataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
    17701 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
    17761 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
    17821 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
    17881 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
    17941 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt ggcgaagcca
    18001 gttaaagacg cgactggcga tgccagtcgc gaaaagaaga gtcaggcttt tacctgctgg
    18061 taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata aaccgccccc
    18121 atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg ccagtctgcg
    18181 cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata agccaggata
    18241 tcagccagat tttttatcca gcccgacggg ctaactttgc gtgccgcgcg cataaacaga
    18301 tcgcggtaca taccgtatgg ccatgcaatt aagatgttcg ccggaatcgc taccaatctg
    18361 gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca catgttcacg
    18421 acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa gcgtgactgc
    18481 ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgttaatg gagatgaaag
    18541 cagtggatct cattgccatc tgcattttcg catagcgtat atcgctggct atgactattc
    18601 aattagtgaa aaatttttat ttagtcagtt tttgtaga
//
LOCUS       DAZFRJ010000077        18579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000077 DAZFRJ010000000
VERSION     DAZFRJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.077"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..884
                     /locus_tag="ACHKUN_002486"
     CDS             <1..884
                     /locus_tag="ACHKUN_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO6660726.1"
                     /translation="PMPRHANPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYIKEVF
                     VSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASSYENDELTYTPGAD
                     DNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLERPVKL
                     MIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQVKATLLSSR
                     YLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDTADRLNYQ
                     KMAQVVDGVINLLYNSK"
     gene            987..1490
                     /locus_tag="ACHKUN_002487"
     CDS             987..1490
                     /locus_tag="ACHKUN_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO6660727.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1557..3014)
                     /gene="nuoN"
                     /locus_tag="ACHKUN_002488"
     CDS             complement(1557..3014)
                     /gene="nuoN"
                     /locus_tag="ACHKUN_002488"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO6660728.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3021..4550)
                     /gene="nuoM"
                     /locus_tag="ACHKUN_002489"
     CDS             complement(3021..4550)
                     /gene="nuoM"
                     /locus_tag="ACHKUN_002489"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO6660729.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFDISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4714..6555)
                     /gene="nuoL"
                     /locus_tag="ACHKUN_002490"
     CDS             complement(4714..6555)
                     /gene="nuoL"
                     /locus_tag="ACHKUN_002490"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO6660730.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6552..6854)
                     /gene="nuoK"
                     /locus_tag="ACHKUN_002491"
     CDS             complement(6552..6854)
                     /gene="nuoK"
                     /locus_tag="ACHKUN_002491"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO6660731.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6851..7405)
                     /gene="nuoJ"
                     /locus_tag="ACHKUN_002492"
     CDS             complement(6851..7405)
                     /gene="nuoJ"
                     /locus_tag="ACHKUN_002492"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO6660732.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7417..7959)
                     /gene="nuoI"
                     /locus_tag="ACHKUN_002493"
     CDS             complement(7417..7959)
                     /gene="nuoI"
                     /locus_tag="ACHKUN_002493"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO6660733.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7974..8951)
                     /gene="nuoH"
                     /locus_tag="ACHKUN_002494"
     CDS             complement(7974..8951)
                     /gene="nuoH"
                     /locus_tag="ACHKUN_002494"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO6660734.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8948..11674)
                     /gene="nuoG"
                     /locus_tag="ACHKUN_002495"
     CDS             complement(8948..11674)
                     /gene="nuoG"
                     /locus_tag="ACHKUN_002495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO6660735.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11727..13064)
                     /gene="nuoF"
                     /locus_tag="ACHKUN_002496"
     CDS             complement(11727..13064)
                     /gene="nuoF"
                     /locus_tag="ACHKUN_002496"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO6660736.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13061..13561)
                     /gene="nuoE"
                     /locus_tag="ACHKUN_002497"
     CDS             complement(13061..13561)
                     /gene="nuoE"
                     /locus_tag="ACHKUN_002497"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO6660737.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13564..15366)
                     /gene="nuoC"
                     /locus_tag="ACHKUN_002498"
     CDS             complement(13564..15366)
                     /gene="nuoC"
                     /locus_tag="ACHKUN_002498"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO6660738.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15460..16122)
                     /gene="nuoB"
                     /locus_tag="ACHKUN_002499"
     CDS             complement(15460..16122)
                     /gene="nuoB"
                     /locus_tag="ACHKUN_002499"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO6660739.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16138..16581)
                     /gene="nuoA"
                     /locus_tag="ACHKUN_002500"
     CDS             complement(16138..16581)
                     /gene="nuoA"
                     /locus_tag="ACHKUN_002500"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO6660740.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17212..18150)
                     /gene="lrhA"
                     /locus_tag="ACHKUN_002501"
     CDS             complement(17212..18150)
                     /gene="lrhA"
                     /locus_tag="ACHKUN_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO6660741.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4642 a         5154 c         4801 g         3982 t
ORIGIN      
        1 cgccaatgcc ccgacacgca aatcctgagc aattagaaaa aacagtgcgt tatcttacac
       61 aaactgtgca tccacgtagt gccgacaata ttgataatct gaataggtcc gcagagtata
      121 taaaagaggt cttcgtcagt agcggtgcca gaattacctc gcaggacgtc cccattacgg
      181 gtggccccta caaaaacatt gttgctgatt atggtcctgc cgatggaccg ctgattatta
      241 ttggtgcgca ctatgactct gccagcagtt atgaaaacga tgaattgacc tatacacctg
      301 gcgcggatga taacgccagc ggcgtggcag gattactcga actggcacgt ttgttacatc
      361 agcaagtacc gaaaacaggc gtgcagctgg tcgcctatgc gtcggaagaa ccgcccttct
      421 ttcgtagcga tgaaatgggg agcgcggtac atgcagcttc gcttgagcgt ccagtaaaat
      481 taatgatagc actggagatg attggctatt acgactctgc gcctggtagc caggattacc
      541 cttacccggc aatgtcctgg ctttatcccg atcggggaga ctttattgcc gtggtcggca
      601 gaatgcagga tatcaacgcc gttcgtcagg taaaagcgac attgttatca tcccggtatt
      661 tatctgttta ttctatgaat gccccagggt ttattcccgg tattgatttc tctgatcacc
      721 ttaattattg gcaacacgat attcccgccg taatgattac tgacaccgct ttttatcgta
      781 ataaacaata ccacttgccc ggtgataccg cagacagatt gaattatcag aaaatggctc
      841 aggtggtgga tggtgttata aatttgttat acaacagtaa ataataaaaa tttctcgagg
      901 cgggtccaga aaaagcgaca tgcacatttt attttccctc gtctgtttta agatgcgaaa
      961 catgaaattc aatggaggaa aaataaatgg gaatgattgg ttattttgca gaaattgact
     1021 cagaaaagat taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg
     1081 acacgctttc aggattacgc cgactggata ttgataaacg ctgggatttt ttacactttg
     1141 ggctgacagg cacctctgct tttgatcccg cgaagaatga tccgctgagt cgtgccgtgc
     1201 ttggtgaaca cagcctcgaa gatggcatag atggcttcct gggattgacc tggaatcagg
     1261 agttagccgc gacaatcgac cgactggaat cactggatcg aagtgagctg cgtaaacagt
     1321 tttccattaa gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt
     1381 tagaggggca actctttgct tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc
     1441 gacgtatgct gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcagg
     1501 gcaccaaaaa aaaccgccga acatgtcggc ggctttctga cttacaaact aacagattac
     1561 atcagcggca ttgctaaccg cacaatgcta atcagcggtt gtggccatac acccagcacc
     1621 agtaccaaca gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgat
     1681 ggtgcatcgc gacccggttg ttccggggcg tgaagataca ggctcaccgc cacgcgcagg
     1741 tagtagtaga ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt
     1801 gcctggacac cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata
     1861 ccggccagag acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac
     1921 agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg
     1981 ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc
     2041 gcttccatcg acatctcacc agtttgcagc gcaatcagcg ccaccagcag atagccgagg
     2101 tgagagatag atgagtaacc gagcagacgt ttgatgttgg tctggctcag cgccatcagg
     2161 ttaccgaaga tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg
     2221 tcacccaccg gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc
     2281 gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac
     2341 aggtggaacg gcaccagcga gagtttgaag ccgaggccaa caatcatcag tccgaaacct
     2401 gccagcaaca gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac
     2461 gacaggtcgc cagactgtgc atacaccagc gccataccaa acagcaggaa agaagacgct
     2521 gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg
     2581 taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa caaagacgcc
     2641 agatggttgg cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag
     2701 aactcatcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg
     2761 aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc
     2821 ggcgtaacgt ccatagcgcc cgcctggccg acaaaccaga gcgaaaccag cgccgcgtta
     2881 agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc
     2941 atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt
     3001 ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc
     3061 tggatattgc caatcgcgga gcgcgaggta tccagaatcg gctgcggata gaagcccagc
     3121 agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc
     3181 agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc
     3241 agcgaataaa cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca
     3301 acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc
     3361 ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac
     3421 agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accacaaaga
     3481 ataaacagac ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag
     3541 gccaactggc tgccggtgta gatagcaatc agcacgaagc ccatgtggga aaccgaggtg
     3601 taggcgatca gacgtttgat atcggtctgg gcgaaggcca tccacgcacc gtagaagatg
     3661 ccgataacac ccagccacat agcaattggc gcgaactctg ccgacgcgtt cgggaacagc
     3721 ggcagggaga aacgcagcaa accgtaagcg gcagttttca gcaagatccc cgcgaggtca
     3781 acggaaccgg cggtcggagc ctgggagtgc gcatccggca gccagccatg cagcggaacc
     3841 accggcattt tgactgcgaa ggcgatgaag aagcccagca ttaacaggta ttccacacca
     3901 ctggacattg gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg
     3961 tagtgaacaa aaaccagcgc caggatggca atcaacatca ccaggccact cgcctgggtg
     4021 taaatgaaga acttggttgc cgccgtgata cgcgttttac cgtcagaggc tttatgcccc
     4081 cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg
     4141 aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg
     4201 tggaagaagc cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg
     4261 agcagaccgg tcagcacgac catcagcagc gacagcccgt caatggcgag atgaatagag
     4321 atatcaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg
     4381 gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat
     4441 cccatggtga tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag
     4501 cacaggaagc cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt
     4561 attccttgta aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc
     4621 tcttcggact gggggtagtg tcggatgcac cgcgaaggcg gcttatccga cctacgttct
     4681 tatatgccca ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag
     4741 caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagatagc cgttctcact
     4801 taacagcaga cctttacctg caaagcggga aaggacagcc gggatgttca tcattgagtt
     4861 cagcggatcg cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc
     4921 atacagccag tcaaatcccc aggcgttgta ccaccaggtg cccagcagac ggcccggcgc
     4981 actgttggcg atggaggtca ccagagtacg tttacccagc cacagccagg ctgccagcag
     5041 aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc
     5101 cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa
     5161 ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg
     5221 agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga
     5281 ggtcataaac gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc
     5341 acccgcgagg atctcatcct tactgaagaa gcccgcagtg accagcggta gtgccgacag
     5401 tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc
     5461 catcttgaag atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa
     5521 cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg
     5581 cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg
     5641 tttgatgtcg gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac
     5701 aatacccacc agatgcagaa cttccggcgt caccaggaac aggccgtggg tacgggcgat
     5761 caggtagaca cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg
     5821 gcccgccatc gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc
     5881 accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc
     5941 tggtgccagt tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa
     6001 cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt tcattgccgc
     6061 tgcgccattc ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac
     6121 gccttcccag ccgaggtaca tcagcagcag gttatcggcg agaaccagaa ctaccatgct
     6181 ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta
     6241 ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag
     6301 cgacaggccg tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt
     6361 ccacagcggc tggctgtatg actgctcgcc gttagcgaag aaatcaacgc cgataaaggc
     6421 ggttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga
     6481 ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa
     6541 ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga
     6601 cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg
     6661 gcgagaatgt acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag
     6721 gccagcgcgg aggcgttaat catgatttcc agaccaatca gcataaacag cagattgcga
     6781 cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt
     6841 tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc
     6901 tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga
     6961 gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca
     7021 ctgctttagc actgattggc gtaccgtcga taccctgatc attaacacca aggatggcgt
     7081 aaacaatcac caccagcatg atggccgaca aaattgccgg accaatccac acctgcggtt
     7141 tcagccactg gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga
     7201 acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt
     7261 aagcgcccag tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca
     7321 gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc
     7381 cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc
     7441 gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc
     7501 catccggtag aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt
     7561 ttcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat
     7621 cgccgtggtc ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttgat
     7681 gcggaaaaat tccgggtacc agcgaccgtc tttggtttct gctttttgca gcgagataca
     7741 gccgaccggg caggctaccg cgcagaggtt acaggctacg caacgctctt cgccgtccgg
     7801 gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg
     7861 gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac
     7921 ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc
     7981 gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag
     8041 attttccagc cgaaggacat tacctggtca taacgcggac gcggtaacga cgcacgaatc
     8101 aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac
     8161 aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc
     8221 ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa
     8281 ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acataccgcc
     8341 acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggataacgtt ccacacatgc
     8401 gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc
     8461 agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc
     8521 agcaacgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga
     8581 cctgccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga
     8641 ctgactggca caatcgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg
     8701 gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc
     8761 atgtccgcaa ccagctggag cgaaccgccc cagccaacac ggttaggtcc gtaacggttc
     8821 tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccgca ggtgacaacc
     8881 accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat
     8941 atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag
     9001 caatgccgga catacccatc ggcaaaccca cctgccctgc cgtcagccct tcagcgattt
     9061 caaccggcag cgtgaccgtg ttgccatcgt aactaaagga gacgcgagcg cctgcgttca
     9121 cgcccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct
     9181 ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtaa tacggcgcga
     9241 tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac
     9301 cattttcgct ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca
     9361 cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg
     9421 gcacttgcga acggtgcgca gtcggctggt tgttaccttc catcgagaag gtgaacatgg
     9481 tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac
     9541 gaccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat
     9601 ccggcgcagc atctttgata cctgccagtt ccgggatttt cgccacaaca gcgtcaatca
     9661 catggtcgag ctgcgtccag tccacttcac ggctcagcag ggtgctgtgc agcgagtgta
     9721 accagcgcca gctttccagc atgacagttt tgctgtcgta ataagcagga tcgtaaacct
     9781 ggaagaaacg ttgggcgcgg ccttcgttgt tgatcaccgt accgtcgctt tcagcaaagc
     9841 tggcagcaga aagtaccaga tgggcgtttt ccataatcgc tgtgcgttga tgatcaacca
     9901 ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcggaa gcgtgacgat
     9961 gcagatcgtt ttccagcacc accaccgcgt cggcgcgtcc ggtttccagt tcggtcaacg
    10021 cttcttcaag cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa
    10081 tcatggtgat accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag
    10141 cctgaatcac ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac
    10201 ctgccagtgc ctgcacgatg acgtcgattt tgctttgcag ctcaggttcg ataccgtcaa
    10261 ccgctggcgc agagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt
    10321 caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgggtgtca tcaacgttgg
    10381 taacaaacag cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt
    10441 cagccacttt ctgtgctgcc gccatttcgc gcgctttacc tttcacagcc tgacgcactg
    10501 ccagcgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt
    10561 aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg
    10621 ccagttgcag acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca
    10681 ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca
    10741 ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg
    10801 taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatccttc aggttgacgt
    10861 aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac
    10921 ggttttcgat acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg
    10981 aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg
    11041 agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt
    11101 cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcaccgtaaa
    11161 cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga
    11221 tgcagcggtt catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt
    11281 tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc
    11341 agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga
    11401 ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg
    11461 aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt
    11521 gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt
    11581 aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct
    11641 cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcagtca
    11701 gagactgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc
    11761 ccattaatca aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg
    11821 aaatatttga tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt
    11881 ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctgacct
    11941 tcaccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc
    12001 gtacaccagc cgcaggattc acgggcgaaa aactcttcca ggttacgcac cagcgacacc
    12061 atgttgatct catggtcaac cgccatcgcc agcgccgtac ccagacggct tcctgcttta
    12121 ccgatacttt cgaattccat cggcaggtca agatgcgctt cggtcaggaa gtcggtgccc
    12181 gcgccgcctg gctgccaggc tttgaatttc agaccgtcac gcataccacc ggcgtaatct
    12241 tcgaggatct cgcgcgcggt agtaccaaac ggcagttccc acaggcccgg atttttcacc
    12301 cgaccggaga agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac
    12361 cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca
    12421 caggtcggtt taccccatgc gccggaggtc gccgggaatg gaggcttcga gcgtgggtta
    12481 gcacgacgtc cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct
    12541 gccccggtat ggacgaacag ttcgaagtca aaacctgttc ccataatatt tttgccaagc
    12601 agacctgctt cggtggcttc ggcaatggca cggcgtagat tcactgccgc ttcgatatat
    12661 tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata
    12721 ccttccacca gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc
    12781 tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc
    12841 aggctccact tcaggccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct
    12901 tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg
    12961 taaccatttt tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc
    13021 caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca
    13081 gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc atcatatttg
    13141 gccctttatc acagttcccc aggcagcagg ttggcagcag cgtaaagcga ccgtcaaacg
    13201 tcgtttgccc tggtttgatg ttcagttttt tctccagcgc cgcctgaata ccctggtaac
    13261 cgttgatgtg acagaccacg ctgtcgcaat aacggatcac atggcgacca accggttggc
    13321 ggaagatctg gctatagaac gttgccacgc cttcgacatc gctcgccgga atacccagca
    13381 catcggcgat cgcgtggatc gcgccgtccg gcacccagcc acgctgcttc tgaacgattt
    13441 tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg
    13501 cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca
    13561 taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc
    13621 agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt
    13681 gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat
    13741 ccccttggtc gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga
    13801 cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg
    13861 cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg
    13921 ctcaagaatg cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca
    13981 gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg
    14041 acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc
    14101 ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat
    14161 ggtattttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa
    14221 ctcacgcagc aggcgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa
    14281 ccacgccggg tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg
    14341 atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat
    14401 gtacagcagg tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac
    14461 gcgatccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt
    14521 aacgcagccg ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg
    14581 gcgctcgccc attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac
    14641 aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt
    14701 cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc
    14761 ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa
    14821 cggcgagaat tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt
    14881 ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc
    14941 ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg
    15001 caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat
    15061 cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg
    15121 ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa
    15181 atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt
    15241 cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc
    15301 atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt
    15361 caccattgca aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag
    15421 gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg
    15481 caggttggtt acggcaatac gttcaccgcg cttgcgttcg cgctctgatt gcatattggc
    15541 gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga
    15601 ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat
    15661 atacacatca accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat
    15721 accaccagag ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc
    15781 atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag
    15841 gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac
    15901 cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca
    15961 aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt
    16021 gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc
    16081 gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta
    16141 gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc
    16201 gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa
    16261 aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca
    16321 tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa
    16381 cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac
    16441 gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca
    16501 acacaggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc
    16561 agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt cactgctctt
    16621 cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaatcact
    16681 gtagaaattc ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg
    16741 tcaactttaa caaaagtttc ttcacattag tttacataat atcaacacca ttagcattta
    16801 atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt
    16861 ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct
    16921 ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattcc ataacaaaaa
    16981 cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga
    17041 ttatttgtgc gttcgggacg tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt
    17101 ctgcactatg cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc
    17161 agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat
    17221 atccctttca atcaacaatg aatcatcccc ttccggagca gacattgggc tgtattgcca
    17281 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    17341 cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact
    17401 cagcacacgc aggtccgggc tcatcatctc aaccggcctt gccgtcacgc caagaccggc
    17461 tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccatggaat
    17521 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa
    17581 tacaagaggg atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    17641 tggcgaggtt cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa
    17701 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    17761 cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    17821 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    17881 tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc
    17941 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    18001 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    18061 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    18121 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    18181 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    18241 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    18301 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata
    18361 tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat
    18421 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    18481 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    18541 aaccttttat ttataagggt cattgcgaat tatctgatg
//
LOCUS       DAZFRJ010000078        18530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000078 DAZFRJ010000000
VERSION     DAZFRJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.078"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(13..636)
                     /gene="rsmG"
                     /locus_tag="ACHKUN_002502"
     CDS             complement(13..636)
                     /gene="rsmG"
                     /locus_tag="ACHKUN_002502"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO6660742.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGH
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(700..2589)
                     /gene="mnmG"
                     /locus_tag="ACHKUN_002503"
     CDS             complement(700..2589)
                     /gene="mnmG"
                     /locus_tag="ACHKUN_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO6660743.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2967..3410)
                     /gene="mioC"
                     /locus_tag="ACHKUN_002504"
     CDS             complement(2967..3410)
                     /gene="mioC"
                     /locus_tag="ACHKUN_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO6660744.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3500..3958)
                     /gene="asnC"
                     /locus_tag="ACHKUN_002505"
     CDS             complement(3500..3958)
                     /gene="asnC"
                     /locus_tag="ACHKUN_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO6660745.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4110..5102
                     /gene="asnA"
                     /locus_tag="ACHKUN_002506"
     CDS             4110..5102
                     /gene="asnA"
                     /locus_tag="ACHKUN_002506"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO6660746.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5107..6558)
                     /gene="viaA"
                     /locus_tag="ACHKUN_002507"
     CDS             complement(5107..6558)
                     /gene="viaA"
                     /locus_tag="ACHKUN_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO6660747.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6552..8048)
                     /gene="ravA"
                     /locus_tag="ACHKUN_002508"
     CDS             complement(6552..8048)
                     /gene="ravA"
                     /locus_tag="ACHKUN_002508"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO6660748.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8271..10139
                     /gene="kup"
                     /locus_tag="ACHKUN_002509"
     CDS             8271..10139
                     /gene="kup"
                     /locus_tag="ACHKUN_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO6660749.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10306..10725
                     /gene="rbsD"
                     /locus_tag="ACHKUN_002510"
     CDS             10306..10725
                     /gene="rbsD"
                     /locus_tag="ACHKUN_002510"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO6660750.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10733..12238
                     /gene="rbsA"
                     /locus_tag="ACHKUN_002511"
     CDS             10733..12238
                     /gene="rbsA"
                     /locus_tag="ACHKUN_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO6660751.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12243..13208
                     /gene="rbsC"
                     /locus_tag="ACHKUN_002512"
     CDS             12243..13208
                     /gene="rbsC"
                     /locus_tag="ACHKUN_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO6660752.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13233..14123
                     /gene="rbsB"
                     /locus_tag="ACHKUN_002513"
     CDS             13233..14123
                     /gene="rbsB"
                     /locus_tag="ACHKUN_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO6660753.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14249..15178
                     /gene="rbsK"
                     /locus_tag="ACHKUN_002514"
     CDS             14249..15178
                     /gene="rbsK"
                     /locus_tag="ACHKUN_002514"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6660754.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15182..16174
                     /gene="rbsR"
                     /locus_tag="ACHKUN_002515"
     CDS             15182..16174
                     /gene="rbsR"
                     /locus_tag="ACHKUN_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO6660755.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16140..17567)
                     /gene="mdtD"
                     /locus_tag="ACHKUN_002516"
     CDS             complement(16140..17567)
                     /gene="mdtD"
                     /locus_tag="ACHKUN_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO6660756.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17590..18282)
                     /gene="yieP"
                     /locus_tag="ACHKUN_002517"
     CDS             complement(17590..18282)
                     /gene="yieP"
                     /locus_tag="ACHKUN_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO6660757.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4470 a         4593 c         4828 g         4639 t
ORIGIN      
        1 ttttgataaa aattaaattt tatttgcttt aatcaccacc agatgacgtt cgccatccaa
       61 ggctggaacc tgaagtttaa ccactgattc gacctgatat tcttcgggca acaaagcgat
      121 ttcatcttcc ggcatttgcc ctttcagcgc gtagaaatgg ccttgctcac caggaagatg
      181 gtggcaccag ctcaccatat cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa
      241 tggcggctct gaaggaaact cttctaccct gctctgtact ggttcaatat tctccagttt
      301 aagctcatgt tgcacctgac gaaggaaacg cacgcgttta ccaaggctat ccaacagagt
      361 gaaatgggct tcaggacgca cgatagagag tggaatgcct ggcagtcctg gtccggtgcc
      421 gacatcgata aaccgttcac cttgcagata cggtgccacc acaatgctat cgagaatatg
      481 gcgtaccagc atctcattag gatcgcggac cgaagtcagg ttgtacgctt tgttccattt
      541 atgcagcata ttcacgtagg caataagctg gtttttctgg tgatcggtaa gcgaaatacc
      601 tgcgtctttc agcagtaagg agagtttgtt gagcacggtg attacctgtt cttgatgcgt
      661 tgcctggtaa gcgggtgctt accaggcatt tttaatgcgt tatgcgctac gacgcagcat
      721 accctgtttt ttcagccaca ccagcagaat ggagatggcc gcaggcgtga cgccagaaat
      781 acgcgaagcc tggccgatag aggctggttt gtgatcgtta agtttggcga tcacttcgtt
      841 agaaagaccg gatacctggc ggtaatccag tgtcgcgggt agcagggtgt tctcgttacg
      901 cagctgcttt tcgatctcat cttgctggcg cgcgatataa ccttcgtatt taacctgaat
      961 ctcaacctgt tccgccgcct gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt
     1021 taatttttca taagtcattt ccggacgacg cagcagatct tcaccactgg cttcacggga
     1081 aagcggcgca gtcaggtgag cattcacttc ggctgcagct tccgccgacg gggttaccca
     1141 ggtcgatttc agacgctgac gctcacgctc gatattctca agtttctcgt taaagcgcgc
     1201 ccaacgttcg tcatccacca ggcccagttc acgaccgatt tcagtcaaac gcagatccgc
     1261 attatcttcg cgtagcatca gacgatattc tgcgcgcgaa gtaaacatac gatacggttc
     1321 tttggttcct aaagtgcaca ggtcatcaac tagtacgccg agatacgcct gagaacgtgc
     1381 cggagcccaa ccttctttgt cagcagacag acgggcagcg ttaagaccgg ccagcaaacc
     1441 ttgcgcagcg gcttcttcgt aaccggtagt gccgttaatc tgaccagcaa agaacagccc
     1501 ctggataaac ttgctctcca gcgtcggttt caggtcgcga ggatcgaaga agtcatactc
     1561 aatggcataa cccggacgca cgatcttcgc gttttccatc ccctgcatag agcggacgat
     1621 ttgcatctgc acatcgaacg gcaggctggt ggagataccg ttcggataaa tttcattaga
     1681 ggtcagtcct tccggttcaa ggaagatctg atgctgattt ctgtcggcga agcgcataac
     1741 tttgtcttcg atcgacgggc agtagcgtgg gccgacacct tcgatcaccc ctgcatacat
     1801 tgggctacga tcgaggttac tgcggatcac atcatgggtt ttctcgttgg tatgagtgat
     1861 ataacacggc acctgctggg gatgctggga cgcattgccc ataaacgaga ataccggcat
     1921 tgggttatcg ccatgctgtt gcgccagcac actaaagtcg atagttcgag catcaatacg
     1981 cggtggtgtc ccggttttca gacgaccaac gcgcagcggc agttcacgca aacggcgaga
     2041 aagcggaatg gacggcggat caccagcacg gccaccgctg taattatcca gaccgatatg
     2101 aattttaccg tcgaggaacg tcccaacggt gagcacgacg gctttggcac ggaacttcag
     2161 tcccatttgg gtaacagcac cgaccacgcg atcgttttcg acaataagat cttcaaccgc
     2221 ctgctggaag atcatcaggt tcggttggtt ctccagcgcc gtacgtaccg cctgacggta
     2281 gagcacacga tccgcctgag ctcgggtagc gcgaaccgcc ggtcctttgc ttgcgtttag
     2341 tatcctaaac tggatacccg cttgatcgat cgctttcgcc atcagaccgc cgagtgcatc
     2401 cacttctttt accagatgtc ccttcccaat accgccgatc gccgggttgc agctcatctg
     2461 ccccagagtg tcgatattgt gtgtcaaaag cagagtctgt tgacccatac gcgccgcggc
     2521 catcgcggcc tcggtgcctg catgaccccc gccaatgatg atgacgtcaa aaggatccgg
     2581 ataaaacatg gtgattgcct cgcataacgc ggtatgaaaa tggattgaag cccgggccgt
     2641 ggattctact caactttgtc ggcttgagaa agacctggga tcctgggtat taaaaagaag
     2701 atctatttat ttagagatct gttctattgt gatctcttat taggatcgca ctccctgtgg
     2761 ataacaagga tccggctttt aagatcaaca acctggaaag gatcattaac tgtgaatgat
     2821 cggtgatcct ggaccgtata agctgggatc agaatgaggg gttatacaca actcaaaaac
     2881 tgaacaaccg ttgttccttg gataactacc ggttgatcca agcttcctga cagagttatc
     2941 cacagtagat cgcacgatct gtatacttat ttgagtaaat taatccacga tcccagccat
     3001 tcttctgccg gatcttccgg aatgtcgtga tcaagaatgt tgatcttcag tgtttcgcct
     3061 gtctgttttg caccggaatt tttgagttct gcctcgagtt tatcgatagc cccacaaaag
     3121 gtgtcatatt cacgactacc aataccgatt gcgccaaagc ggaccgcaga aagatcgggc
     3181 ttctgttcct gcaatgcttc atagaaagga gaaaggttgt ccggaatatc tccggcaccg
     3241 tgggtggagc tgataatcag ccagatcccc gaggcagata aatcttccaa cagcggaccg
     3301 tgcagcgttt cggtggtaaa acccgcctct tccagctttt cagccaggtg ttctgctaca
     3361 tattcggcac cgccgagggt gctgccgctg ataagagtga tatctgccat aaaccgccac
     3421 ctttattaag agtggcgtat tgtacgctgt gaacgcgttg ggatctacct gtggaaaagt
     3481 atgggattaa aaaagccgat tatggcttga tggtacgcat gatcgggttt tgcaggacga
     3541 tcaatgtctc ggtggactga atttcatcaa ttgtttggat cttgttgata agtacatgct
     3601 ggagagcgtc gatcgaacgg cacatcactt ttataaagat gctgtagtgg ccggttgtgt
     3661 aataggcttc agtgacttca tcaaggcttt ccagctttgc cagcgcggaa gggtagtctt
     3721 tggcgctctt taatataatg ccgataaagc agcctacgtc ataaccgagc tgcttcgggc
     3781 tgacatcaat acgcgccccg gtaatgatcc ccgcctgctt cattttctct actcgaacgt
     3841 gaatcgtccc cggactgacg ccaaattgtt tcgccagttc ggcgtaagcg gtgcgcgcat
     3901 tgcccattaa tgcttccagg atgccacggt ccagattgtc gatcagataa ttttccatag
     3961 gattttctta tgcagattga tgattcattc tattttagcc ttctttttta atgaatcaaa
     4021 agtgtgttag gctttttatt gaatgattat tgcatgtgtg tcggtttttg ttgcttaatc
     4081 ataagcaaca ggacgcagga gtataaaaaa tgaaaaccgc ttacattgcc aaacaacgcc
     4141 aaattagctt cgtgaaatct cacttttctc gccaactgga agaacgtctg gggctgatcg
     4201 aagtccaggc gccgattctt agccgtgtgg gggatggcac gcaggataac ttgtcgggct
     4261 gtgaaaaagc ggtgcaggta aaagtgaaag ctctgcctga tgcccagttc gaagtggttc
     4321 attcactggc gaagtggaaa cgtcagacct tagggcaaca cgacttcagc gcgggcgaag
     4381 ggctgtacac gcacatgaaa gcccttcgcc ccgatgaaga gcgtctttct ccgttgcact
     4441 cggtctatgt tgaccagtgg gactgggaac gcgtaatggg cgacggtgag cgtcaattct
     4501 cgactctgaa aagcacggta gaggcgatct gggcgggaat taaagcaacc gaagctgcag
     4561 ttagcgaaga gtttggtttg gcaccgttcc tgccggatca gatccacttc gtacacagcc
     4621 aggagttact gtctcgttat ccggatctcg atgccaaagg gcgtgagcgg gcgattgcga
     4681 aagatcttgg cgcggtattc ctcgtcggga ttggcggcaa gctgagtgat ggtcatcgtc
     4741 acgacgtgcg cgcaccggat tatgatgact ggagcacccc gtcagagctg ggccatgcgg
     4801 gtctgaacgg cgatattctg gtatggaacc ctgtactgga agatgcgttt gagctttctt
     4861 ccatggggat ccgcgtggat gccgacacgt tgaagcatca gttggcgctg accggtgatg
     4921 aagatcgtct gcagctggaa tggcatcagg cgctgctgcg cggtgaaatg ccgcagacca
     4981 tcggcggcgg tatcggccag tctcgtttga ccatgctgct gctgcaactg ccgcatatcg
     5041 gccaggttca gtgtggagta tggccagctg ctgttcgcga gagcgtccct tctctgctgt
     5101 aataatttat cgccgccagc gtctgagcag gcggcttcgc atcccggtat caaagcgcca
     5161 gatatgatcg aaaatgcgca tgatgccggg tttgccgtgt gccgacatcg ccacggcatg
     5221 aaagcgatgc tgatgtaccc gctgcagctc tttcacttta ctcgtcacgt cgtcaggcaa
     5281 ccgctgagcg ataaaatcag aaatcaccac cgcatcggca tcaaaccatt ccctgctttg
     5341 caagcgttcc ataatggcgc gaaaacaact ggcaagatcg gtgccgccac gaaactgctg
     5401 gcttaaaaaa cggattgctt gttcgatgcc ttgtgggcct gaaagctcat aacggacgat
     5461 ctcggtggaa aatagcataa tatagcagcg ccggttttca gcgagagcaa tgcgcatcaa
     5521 ggccaggcag aacgctttcg cacactgttc attaaagccg cccattgagc cggaagtatc
     5581 cacacagaca ataaacggcc cgcgcggctg ttcatcgtaa tctttatgta ccaccggacg
     5641 ttcgatcact ttttcacgcc acgactcacc gtgcaggcga taggtgagca actgtttttc
     5701 caccagccga cggtaaaact catactccag ttccgttatc cctagtgtcg ccagttctgg
     5761 cggcagaaga cgtaaaatat catcgctttg ttgcagacca tcaacctgct caggaaccgt
     5821 cgccggttcg cgcaccatgg tgcggaaggt ttccatctgc gcatcgttgc gcggtattga
     5881 tttggcttcc cgagaacgcc ccagctgctc tgccaggcgt ttcagttccg gctgttcgtt
     5941 aagaaattca ccgtatttca caatcaactg atagtcgcca cgtttaagct gaccggcgct
     6001 catatcccac agacgaccag ctgcagtatt gttatctgcg agaatcggtt caagttgtcc
     6061 gctcagcgtc atgcgttcct gaacttcact caacagttgt tcgcgttctt cttctaatag
     6121 ctgttgattt aacgtcgttg cttgcacgat cagacttaaa cgccaacgct ggagaaaaag
     6181 cgtgtgtaac gctgaagtga tcgtgctgtt agcatcaacc aactgtcggg cttgttctgc
     6241 ccatggagaa ttcagacgat gcagtaagtc caggatctgt ggtagctgca caataaactg
     6301 tggcgtggag aggagctggc tttgctgata gcacatcacc tcttcggtga gttccggcgg
     6361 gactcgggca tctttcagcc gactgcgcag cgcttcacgc cagcggggaa catcatcagt
     6421 gattgccgcc ttcaggcgtg ggaatttttc aaagaagacc gccagctgcg gtgaggccag
     6481 cagtgcgatg atcatctctt cgatcaatcc ctcttcgctg acggccagca tcacattaag
     6541 cgtatccagc gttagcattg ttgtgcctgg cgaatctgtg caccgacatc ctgtaggctg
     6601 gcttcaatgc gacctaacca gtcgccaggg ataaacagac atttttgctg ttcgctgaat
     6661 aaagcgtgtt gcttgcgcca gtcgctttca agctcttcca gttgttgttt tatttcacca
     6721 ggcagacctt cagccgatga accgggaagt gccagcgtac tgccttgtaa acttacatcg
     6781 cgaacgacaa gatgttgggc gctatcaact tccagattca gtttctgggc aaagccgata
     6841 ccgttcagtt taccgcgaat ttcaccacct ttgctcagcc actgttccag cgcgctacgc
     6901 tcaaaggaga tatgaaccac ttccatatca tgcagtttta acggtttttg cagcagtaga
     6961 gtcagagtgg aagcagtaac gttaacagga agttgatact gctgacgacg gctgaaaatg
     7021 ccgcccagac gaattaccgt taaggctgtt ttatcgcttt gttgctgctg gagttgcagg
     7081 tgacgttgca caatcgcgcc aaggcgagtc aacatccctt gctgttgcca ggcgtgaccg
     7141 gtcatcaata catcaatttg ttgttgtatc aaattcaggc tttgcgcgtc ataccacagg
     7201 caatctttca gcaaaatgag atctaccggg gcaacagcac tgcgaccgct aaaaaaggcg
     7261 ctggcctgca ataatcggat cgcttttttc cagcgacgat ccgagacata aggcgcatcc
     7321 ggtaatttat ccagttgctg gcgcagcata aaaatcagct caaatacatg atcgggcagc
     7381 gtaatttcac caatctcttt ctgccagcgt tcatattctt catctgtgac ctgcaaggca
     7441 tcaggaaccg gattgtcgtt ttcatcctgt tgactggtca gcatggagcg gaaattcgct
     7501 ttatcctgca ctttatctaa ccacagacga atcagcatgc ggtcatataa cgcttccaga
     7561 ctgctgtctg cttccggcag ctcgttggag gccgccacca gcagacgcat cgggattttt
     7621 tctacgtgtg caccgttgcg gaactggcgc tcgttaatgg cggtgagcaa ggtattaaga
     7681 attgccgggc ccgctttcca gatctcatcc agaaagacaa tttctgcttc cggcaggtaa
     7741 ccgctggtta aacgttcata gcgcccttca tcttttagcg cctgaataga aaggggacca
     7801 aaaacttctt ccggcgtgga gaagcgggtc atcagatatt caaacgcgcg ggcattctga
     7861 aaggcgaatt ttaagcgccg ggcgatcaaa cttttggcaa tacctggcgg gccaaggagg
     7921 aacacacttt caccacttaa tgccgctaat aaacacaagc ggatggcgtg gctacgttca
     7981 taaagcccct tttccaacga actgctcagg cgggaaattc tttccgctaa taaatgaggg
     8041 tgagccataa tgaagtggcg tcctttcgtc aaaagttctg cgtaaattgc gagtatagac
     8101 gtttcttgct ggtggctaaa atagtctcaa agggggggta tttttctttg agccaggtta
     8161 atgtggccgc atttaggagt acgattttgc cgttaatcgt gcatactgtg cgcttttttg
     8221 tgggccaagg gactaagcac acatttcata tttcaacgaa agactagtct atgagcactg
     8281 ataataagca atcattgccc gcgattaccc tcgcggcgat tggagttgtc tacggcgata
     8341 ttggtaccag cccgttatat acacttcgtg aatgtttgtc cggccagttt ggttttggcg
     8401 ttgaacgcga taccgtgttt ggctttttat cgctgatctt ctggctgcta atctttgtgg
     8461 tttccattaa atatctcacc ttcgtgatgc gggcagataa cgccggtgag ggggggatcc
     8521 tgacgttgat gtcgcttgcc gggcgtaata cgtcggcgcg aaccacatca atgctggtga
     8581 ttatggggct aatcggcggc agctttttct atggtgaagt cgtcataaca cccgctattt
     8641 cggtgatgtc agccattgaa ggtctggaaa tcgtcgcccc gcagctggat acctggatag
     8701 ttcccctctc aattatcgtt ctcactttat tatttatgat tcaaaaacat ggcacggcta
     8761 tggtcggtaa gctgtttgcg ccgatcatgc tgacctggtt tttgattctg gcaggactgg
     8821 ggttacgtag cattattgct aacccggaag tgctgcatgc gctgaatcca atgtgggcgg
     8881 tgcatttctt ccttgaatac aaaacggttt cttttattgc attaggggca gtggtgttgt
     8941 cgattacggg ggtcgaggcg ctgtatgctg atatggggca ctttggtaag ttccctattc
     9001 gcctggcgtg gttcaccgtc gtattacctt ccttgaccct taattacttc ggtcagggag
     9061 cgctgttgtt aaagaacccg gaagcgatta agaacccgtt cttcctgttg gcaccggact
     9121 gggcgctgat cccgctgctg atcatcgccg cactggcgac ggtaattgcc tcgcaggcgg
     9181 ttatctctgg cgtcttctca ttgacgcgtc aggcggtacg tctgggatat ttgtcgccga
     9241 tgcgcattat tcacacctcc gaaatggagt cagggcaaat ctatattccg tttgtgaact
     9301 ggatgctcta tgtcgcggtc gtgattgtga ttgtcagctt tgagcactcc agcaacctgg
     9361 cggcggcgta cgggattgcg gtgaccggaa ccatggtgct gacgtctatt ctctcgacta
     9421 ccgtggcacg tcagaactgg cactggaata agtattttgt tgcgctgatc ctgattgctt
     9481 tcctttgtgt cgatattcca ttgttcaccg ctaacctcga taaactgctc tccggcggct
     9541 ggttgccatt gagcctcggt actgtgatgt ttatcgtgat gaccacctgg aagagcgagc
     9601 gtttccgctt gctgcggcgg atgcatgaac atggtaactc tctggaagcg atgattgctt
     9661 cgctggagaa atcaccgcct gttcgcgtgc ccgggaccgc ggtgtatatg tcgcgtgcaa
     9721 tcaacgtcat tccctttgcg ctgatgcata accttaaaca taacaaggta ttgcatgagc
     9781 gggtgattct gttaactctg cgtaccgaag acgcgccata tgtccataat gtccgtcggg
     9841 tacagattga acaactgtcg cccactttct ggcgcgtggt ggcaagttat ggttggcgag
     9901 aaacgccaaa cgtggaagaa gttttccacc gctgcggtct ggaaggatta agttgccgga
     9961 tgatggaaac ctccttcttt atgtcgcatg agtcgttgat cctcggcaaa cgcccgtggt
    10021 atttgcgtct gcgcggcaag ctgtacttgc tgctgcaacg taatgcgctg cgtgcaccag
    10081 atcaatttga aattccgcca aacagggtta tcgaactggg tactcaggtc gaaatctaac
    10141 gccagacgcc tcctttctcc ataagggggc gtttttgttt tcatggttaa tcaccatgta
    10201 aaacgtttcg aggttgatca catttccgta acgtcacgat ggttttccca actcagtcag
    10261 gattaaactg cgggtcagcg aaacgtttcg ctgatggaga aaaaaatgaa aaaaggcacc
    10321 gttcttaatt ctgatatttc atcggtgatc tcccgtctgg gacataccga tacgctggtg
    10381 gtgtgtgatg ctggtttacc tatccccaaa agtacaacgc gtatcgatat ggcattaacc
    10441 cagggtgtac cttcttttat gcaggtgctg ggcgtcgtca caaatgaaat gcaggtcgag
    10501 gctgccatta tcgcggaaga gatcaaacaa cataatccgc aactccacga aacgttgctc
    10561 actcaccttg agcagctgca aaaacaccag ggaaatacca ttgaaattcg ttacaccacg
    10621 catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg taattcgcag cggagaatgt
    10681 tctccgtatg cgaatatcat tctctgtgct ggcgtgacgt tctgaggccg tcatggaagc
    10741 attacttcag cttaaaggca tcgataaagc cttcccgggc gtaaaagccc tctcgggcgc
    10801 agcgttaaat gtctatccgg gccgcgtgat ggcgctggtg ggcgaaaacg gcgcgggtaa
    10861 atccaccatg atgaaagtgc ttactggtat ctatgctcgc gatgccggca cgcttttatg
    10921 gctggggaaa gaaacgacat ttaccgggcc gaaatcttcc caggaagccg ggattgggat
    10981 tatccatcag gaactgaacc tgatcccgca gttgaccatt gccgaaaaca ttttcctcgg
    11041 tcgtgagttt gttaatcgct ttggcaaaat tgactggaaa accatgtatg ccgaagcgga
    11101 taaattgctg gctaaactta acctgcgctt taaaagcgac aagctggtgg gcgatctttc
    11161 catcggtgac cagcaaatgg ttgaaatcgc caaagtgctg agctttgagt cgaaagtcat
    11221 cattatggat gaaccgaccg atgcgctgac cgataccgaa accgaatccc tgttccgcgt
    11281 catccgcgaa ctaaaatcgc aaggccgcgg tattgtctat atctcccacc gcatgaaaga
    11341 aatcttcgag atttgcgatg acgttaccgt ttttcgtgac gggcaattta ttgctgagcg
    11401 cgaagtggca tcactgaccg aagattcgct gattgagatg atggtgggcc gcaagctgga
    11461 agatcaatat ccacacctgg acaaagcgcc gggagatatc cgcctgaaag tcgataatct
    11521 ctgcggacct ggcgttaacg atgtctcttt tactttacgc aaaggcgaaa ttcttggcgt
    11581 ctctggtttg atgggcgcag gtcgtaccga actgatgaaa gtgctctacg gcgcactacc
    11641 gcgcaccagc ggttacgtca ccctggatgg gcatgaagtc gttacccgtt caccgcagga
    11701 tggcctggca aacggcattg tgtatatctc cgaagaccgt aaacgtgacg gtttagtgtt
    11761 gggcatgtca gtaaaagaga acatgtcgct gacagcgctg cgctacttca gccgcgctgg
    11821 cggcagtttg aagcatgccg atgaacagca ggctgtgagt gatttcattc gtctgtttaa
    11881 tgtgaaaaca ccgtcgatgg aacaggcaat tggtctgctt tccggtggca atcagcaaaa
    11941 agtggcgatt gcccgcggtc tgatgacacg ccccaaagtg ttgatccttg atgaacctac
    12001 ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa ctgattaacc agttcaaagc
    12061 cgatggcttg agcatcattc tggtgtcatc ggagatgcca gaagtattag gcatgagcga
    12121 tcgcatcatc gtcatgcatg aagggcatct cagcggggaa tttactcgtg agcaggccac
    12181 ccaggaagtg ttaatggctg ccgctgtggg caagcttaat cgcgtgaatc aggagtaaaa
    12241 aaatgacaac ccagactgtc tctggtcgcc gttatttcac gaaagcgtgg ctgatggagc
    12301 agaaatcgct tatcgctctg ctggtgctga tcgcgattgt ctcgacgtta agcccgaact
    12361 ttttcaccat caataactta ttcaatattc tccagcaaac ctcggtgaac gccattatgg
    12421 cggtcgggat gacgctggtg atcctgacgt cgggcatcga cttgtctgta ggttctctgt
    12481 tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat tgaagtcaat gcgctggtgg
    12541 ctgtcgctgc cgctctcgcg ttaggtgccg caattggtgc ggtaaccggg gtgattgtag
    12601 cgaaaggtcg cgttcaggcg tttatcgcta cgctggttat gatgctttta ctgcgcggcg
    12661 tgaccatggt ttataccaac ggtagcccag tgaataccgg ctttactgag aacgccgatc
    12721 tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc gacgccagtc tggatcatgg
    12781 ggattgtctt cctcgcggcc tggtacatgc tgcatcacac gcgtctgggg cgttacatct
    12841 acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg tatcaacgtc aataaaatca
    12901 aaatcatcgt ctattctctt tgtggtctgc tggcatcgct ggccgggatc attgaagtgg
    12961 cgcgtctctc ctccgcacaa cccacggcgg ggactggcta tgagctggat gctattgctg
    13021 cggtggttct gggcggtacg agtctggcgg gcggaaaagg tcgcattgtt gggacgttga
    13081 tcggcgcatt aattcttggc ttccttaata atggattgaa tttgttaggt gtttcctcct
    13141 attaccagat gatcgtcaaa gcggtggtga ttttgctggc ggtgctggta gacaacaaaa
    13201 agcagtaata acgactacag gacatcttga atatgaacat gaaaaaactg gctaccctgg
    13261 tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc gatggcaaaa gacaccatcg
    13321 cgctggtggt ctccacgctt aacaacccgt tctttgtatc gctgaaagat ggcgcgcaga
    13381 aagaggcgga taagcttggc tataacctgg tggtgctgga ctcccagaac aacccggcga
    13441 aagagctggc gaacgtgcag gacttaaccg ttcgcggcac caaaattctg ctgattaacc
    13501 cgaccgactc cgacgcagta ggtaatgctg tgaagatggc taaccaggcg aacatcccgg
    13561 ttatcactct tgaccgccag gcaacgaaag gtgaagtggt gagccacatt gcttctgata
    13621 acgtactggg tggcaaaatc gccggtgatt acatcgcgaa gaaagcgggt gaaggtgcca
    13681 aagttatcga gctgcaaggc attgctggta catccgcagc ccgtgaacgt ggcgaaggct
    13741 tccagcaggc cgttgctgct cacaagttta atgttcttgc cagccagcca gcagattttg
    13801 atcgcactaa aggtttgaac gtaatgcaga acctgttgac cgctcatccg gatgttcagg
    13861 ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct gcgcgcactg caaactgccg
    13921 gtaaatcgga tgtgatggtc gtcggatttg acggtacacc ggatggcgaa aaagcggtga
    13981 atgatggcaa actagcagcg actatcgctc agctacccga tcagattggc gcgaaaggcg
    14041 tcgaaaccgc agataaagtg ctgaaaggcg agaaagttca ggctaagtat ccggttgatc
    14101 tgaaactggt tgttaagcag tagttttaat caggttgtat gacctgatgg tgacataaat
    14161 acgtcatcga cagatgaacg tgtaatataa agaaaagcag ggcacgcgcc accctaacac
    14221 ggtggcgcat tttatggaca tcccgaatat gcaaaacgca ggcagcctcg ttgttcttgg
    14281 cagcattaat gctgaccaca ttcttaatct tcaatctttt cctactccag gcgaaaccgt
    14341 aaccggtaac cactatcagg ttgcatttgg cggcaaaggc gcgaatcagg ctgtggctgc
    14401 tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtacg ggtgatgaca gcattggtga
    14461 gagcgttcgc cagcagctcg ccactgataa cattgatatt actccggtca gcgtgatcaa
    14521 aggcgaatca acaggtgtgg cgctgatttt tgttaatggc gaaggtgaga atgtcatcgg
    14581 tattcatgcc ggcgctaatg ctgccctttc cccggcgctg gtggaagcgc aacgtgagcg
    14641 tattgccaac gcgtcagcat tattaatgca gctggaatca ccactcgaaa gtgtgatggc
    14701 agcggcgaaa atcgcccatc aaaataagac tatcgttgcg cttaacccgg ctccggctcg
    14761 cgaacttcct gacgaactgc tggcgctggt ggacattatt acgccaaacg aaacggaagc
    14821 agaaaagctc accggtattc gtgttgaaaa tgatgaagat gcagcgaagg cggcgcaggt
    14881 actgcatgaa aaaggtatcc gtactgtact gattacttta ggaagtcgtg gtgtatgggc
    14941 tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg gtgcaggctg tcgataccat
    15001 tgctgccgga gataccttta acggcgcgtt aatcacggcg ttgctggaag aaaaaccatt
    15061 gccagaggcg attcggtttg cccatgctgc cgctgcgatt gccgtaacac gtaaaggcgc
    15121 acaaccttcc gtaccgtggc gtgaagagat cgacgcattt ttagacaggc agaggtgacg
    15181 cttggctaca atgaaagatg ttgcccgcct ggcgggcgtt tctacctcaa cagtttctca
    15241 cgttatcaat aaagatcgct tcgtcagtga agcgattacc gccaaagttg aagcggcgat
    15301 taaagaactc aattacgcgc catcagccct ggcgcgtagc ctcaaactca atcaaacaca
    15361 taccattggc atgttgatca ctgccagtac caatcctttc tattcagaac tggtgcgtgg
    15421 cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc ctttgcaata ccgaaggaga
    15481 tgaacagcgg atgaatcgca atctggaaac gctgatgcaa aaacgcgttg atggcttgct
    15541 gttactgtgc accgaaacgc atcaaccttc gcgtgaaatc atgcaacgtt atccgacagt
    15601 gcctactgtg atgatggact gggctccgtt cgatggcgac agcgatctta ttcaggataa
    15661 ctcgttgctg ggcggagact tagcaacgca atatctgatc gataaaggtc atacccgtat
    15721 cgcctgtatt accggtccgc tggataaaac tccggcgcgc ctgcggttgg aaggttatcg
    15781 ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc tatgaagtca ctggtgattt
    15841 tgaatttaac ggcgggtttg acgccatgcg ccaactgtta tcacatccgc tgcgtcctca
    15901 ggccgtcttt accggaaatg acgctatggc tgttggcgtt taccaggcgt tatatcaggc
    15961 agagttacag gttccgcagg atatcgcggt gattggctat gacgatatcg aactggcaag
    16021 ctttatgacg ccaccattaa ccactatcca ccaaccgaaa gatgaactgg gggagctggc
    16081 gattgatgta ctcatccatc ggataaccca gccgaccctt cagcaacaac gattacaact
    16141 tactccgatt ctgatggaac gcggttcggc ttagatttac gctgtctttt gatcaaatta
    16201 ttaccatcgg ttgttttcag aagcatgaac attgctgctg aagcaacagt aataatgccc
    16261 atcgtgataa acgtatagtg gaattgttcg acagtcgttg tgccttccat cccttcataa
    16321 acgcgaagga cggccgcact tacagcaacg cctaaactaa tcgacagttg ctgcgtgacc
    16381 gccagaacac tgttaccgct gctggcgttg tcatcggtca gatcggcaag tgtgatggta
    16441 ttcatcgcgg taaattgcgt cgacatagcc atccctaata taaacaacgg caagatcagc
    16501 atccatatcg ccattaccgg tgattgcaaa gagaactgag cgatcattag cccaataatc
    16561 accgtgatcc ccactaacgt atggcgatag cccagacgac gtaagacttg ggtaaccatc
    16621 gattttgcaa taatggaacc taacgctgtc ggtgccatca tacagccggc aataaacgcc
    16681 tgataaccaa atcctacctg taacatcaat ggcataagga acggtacaca accggtcccc
    16741 agacgggttg caatattgcc tacgataccg atcgagaaag tgcgggtttt aaataaatct
    16801 aatgaaatta atgggtttgg cgtgtgtcgc gcatggagaa tatagagaag cagtaacccg
    16861 atgctggtga caattaccgt caaggcaatc cagctggcga caatcttttc cccgaatagc
    16921 tctattccgc ttgagaagag aacaaggctg aggccaaaca gcaaaaagcc agtgatatcg
    16981 aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc gcgcgtaaag aaggcccgca
    17041 atacctatgg ggatattgat taaaaatatc cagtgccagg ttgcccaggt gaccagcacg
    17101 ccgccaagaa cggggcctaa aattggcccc accagacccg gcatggcgac aaaattcaat
    17161 actggaagaa gttcattacg aggataagcg cgcagtaagg ccagccgagc aacaggcatc
    17221 atcattgcgc cgcctatccc ctgaataacc cggaagacaa ccagctgtgg tagcgaatta
    17281 gaaagtgcgc aggccagaga acccaatgtg aacagactca cggcaagggt aaaaatgcga
    17341 cgcgtaccga agcgatcggc tagccatccg cttaccggaa taagcatcgc caccgtcagc
    17401 gtataactga tgatggctga ttgcatcgcg agaggagaac gattaaggct atgagcgatt
    17461 gcgggtaagg cggtattcag aatagtggca tcaagtgcct gcatgaagaa ggccatcgcc
    17521 gcgatccacg gcaaacccgc catactgcgc ttctttttat cgctcattca atgtcctatt
    17581 atcgggttgt cacttatcag gtgagcgtag cagcgcctga caagctttaa atgccgcgtc
    17641 gccatcgctt tggacaatcg catcgacaat cgcctggtgc agatccagct ttatcactgt
    17701 gtcgctggta attgacgtga agtaagtgtg ataaaccgaa tggaatagcg aggcgaatga
    17761 ggtcaaaaac ggattggcgc tcatttcata gatatgctca tgccaggcca tatcgacttc
    17821 gatccagcgt tcacggcgaa agttctcttt taatgccgcc atttcggcca ttaacgtatt
    17881 gagatgcgcc ttctgttccg cggtgccaac cgttgcagcc agtaggcaag cttgcggctc
    17941 cagacagata cgcataacca gaaagtgatc gatgacctga tgaaagttct cttctgtcat
    18001 ccaccaggta agcaattcct gatcaagaaa attccagttt gattgtggca tgacccgggt
    18061 accaattcgc ggtcgcggta aaaccatccc ttttgccgtt aacgttttga ccgcttcgcg
    18121 taccgctgta cgactcactc caaattgctc gcccagctca atctcaccag gcaaaatggt
    18181 gccgggttca tattcacctt ttaagatgcg ttgcgccagc ttctcagcca gaacatacga
    18241 aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc attactaatc ttcctttttc
    18301 tttttattcc tccttagtat gccaccagga agtgtgatta cggttgcaaa aacggcaaat
    18361 tgcttgtttt atggcacatt aacggggctt ttgctgaaaa aatgcgcggt cagaaaatta
    18421 ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg
    18481 aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaattccg
//
LOCUS       DAZFRJ010000079        18509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000079 DAZFRJ010000000
VERSION     DAZFRJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.079"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..1308
                     /locus_tag="ACHKUN_002518"
     CDS             88..1308
                     /locus_tag="ACHKUN_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6660758.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1281..1910
                     /locus_tag="ACHKUN_002519"
     CDS             1281..1910
                     /locus_tag="ACHKUN_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO6660759.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1911..3071)
                     /gene="ybdL"
                     /locus_tag="ACHKUN_002520"
     CDS             complement(1911..3071)
                     /gene="ybdL"
                     /locus_tag="ACHKUN_002520"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO6660760.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3180..4268
                     /gene="hcxA"
                     /locus_tag="ACHKUN_002521"
     CDS             3180..4268
                     /gene="hcxA"
                     /locus_tag="ACHKUN_002521"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO6660761.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4278..4475)
                     /locus_tag="ACHKUN_002522"
     CDS             complement(4278..4475)
                     /locus_tag="ACHKUN_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6660762.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4657..6762)
                     /gene="cstA"
                     /locus_tag="ACHKUN_002523"
     CDS             complement(4657..6762)
                     /gene="cstA"
                     /locus_tag="ACHKUN_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO6660763.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6943..7356)
                     /gene="entH"
                     /locus_tag="ACHKUN_002524"
     CDS             complement(6943..7356)
                     /gene="entH"
                     /locus_tag="ACHKUN_002524"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO6660764.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7359..8105)
                     /gene="entA"
                     /locus_tag="ACHKUN_002525"
     CDS             complement(7359..8105)
                     /gene="entA"
                     /locus_tag="ACHKUN_002525"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO6660765.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8105..8962)
                     /gene="entB"
                     /locus_tag="ACHKUN_002526"
     CDS             complement(8105..8962)
                     /gene="entB"
                     /locus_tag="ACHKUN_002526"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO6660766.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8976..10586)
                     /gene="entE"
                     /locus_tag="ACHKUN_002527"
     CDS             complement(8976..10586)
                     /gene="entE"
                     /locus_tag="ACHKUN_002527"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO6660767.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10596..11771)
                     /gene="entC"
                     /locus_tag="ACHKUN_002528"
     CDS             complement(10596..11771)
                     /gene="entC"
                     /locus_tag="ACHKUN_002528"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO6660768.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12140..13096
                     /gene="fepB"
                     /locus_tag="ACHKUN_002529"
     CDS             12140..13096
                     /gene="fepB"
                     /locus_tag="ACHKUN_002529"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6660769.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13100..14350)
                     /gene="entS"
                     /locus_tag="ACHKUN_002530"
     CDS             complement(13100..14350)
                     /gene="entS"
                     /locus_tag="ACHKUN_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO6660770.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14461..15465
                     /gene="fepD"
                     /locus_tag="ACHKUN_002531"
     CDS             14461..15465
                     /gene="fepD"
                     /locus_tag="ACHKUN_002531"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO6660771.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15462..16454
                     /gene="fepG"
                     /locus_tag="ACHKUN_002532"
     CDS             15462..16454
                     /gene="fepG"
                     /locus_tag="ACHKUN_002532"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO6660772.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16451..17266
                     /gene="fepC"
                     /locus_tag="ACHKUN_002533"
     CDS             16451..17266
                     /gene="fepC"
                     /locus_tag="ACHKUN_002533"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6660773.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17263..18396)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUN_002534"
     CDS             complement(17263..18396)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKUN_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO6660774.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4351 a         5243 c         4784 g         4131 t
ORIGIN      
        1 ataaaacact ctgctttgca gtgtttactt tataattaac agacttatct cactgatcac
       61 cctgtaacgt tcagagagcg tcttcagatg tctatttata aaattcctct tccgctcaat
      121 attctggaag ctgcacgaga gcgtattacc tggacactca acaccctacc ccgcgtttgt
      181 gtctcatttt ctggcggcaa agactctggt ttaatgctgc atctgacagc cgaacttgcc
      241 cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact gggaggcgca attctcttgc
      301 accattaact atgttcagtc cctgcgcgag ttgtacaccg atgtcatcga agagttttac
      361 tgggttgcgc tcccgcttac gacgcaaaat tccctttcac aataccaacc cgaatggcag
      421 tgctgggaac ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat aaccgatcct
      481 gactttttct gcttttacca gccaggcatg accttcgaac aatttgtacg tgagtttgcc
      541 gaatggtttt cacaaaaacg tccggcggcg atgatgatcg gcatccgtgc ggatgagtcc
      601 tacaaccgtt ttgtcgccat cgccagttta aataaacaac gttttgccga cgataaacca
      661 tggactacgg ccgcaccagg cggtcatagc tggtacattt accccattta cgactggaaa
      721 gtggctgata tctggacctg gtatgcaaat catcaaaatc tctgcaaccc actgtataac
      781 ctaatgtatc aggcaggcgt tcctctgcgt catatgcgaa tttgcgaacc ttttggcccg
      841 gagcaacgac aaggattatg gctctatcac gttatcgaac cggatcgctg ggctgctatg
      901 tgcgcacgag tcagcggggt aaaaagtggc ggcatttacg ccggacatga taatcatttc
      961 tatgggcatc ggaaaatcct caagccagaa catttagact ggcaagaata tgcattattg
     1021 ctgctcaata gcatgccgga aaaaacagct gagcattacc gcaataaaat cgccatttat
     1081 ttgcactggt atcagaaaaa aggcatcgag gttccacaaa cccagcaagg ggacattggc
     1141 gcgaaagata tcccctcctg gcggcggata tgcaaagttt tactcaataa cgattactgg
     1201 tgtcgggcat tatcatttag tcctacgaaa gcgaagaact atcagcgtta taacgaacgg
     1261 ataaaaggaa aacgtcagga atgggggata ctatgcaaca acgattaacg caggatttaa
     1321 cgcaatttct cgccagcttg ccggaagatg atcgcatcaa agcgattaat gaaatccgca
     1381 tggcgatcca tcaggttagc ccctttcgtg aagagccagt ggattgcgtt ctgtgggtta
     1441 aaaacagcca acttatgcct aatgattaca acccaaacaa cgtggcacca cccgaaaaaa
     1501 aactgctgaa aaaatccatt gaaatagatg gttttaccca acccattgtg gtcacgcata
     1561 cggataaaaa tgccctggaa atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct
     1621 catcatcatt aaaattacgc ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc
     1681 gtaatcaacg catcgccgcg actattcgtc ataatcgcgc tcgtggacgc catcaaatca
     1741 ccgccatgtc ggaaatcgtc cgtgaactta gccagttggg atgggacgat aataaaattg
     1801 gcaaagagct aggaatggac agcgacgaag tgctgcgcct gaagcaaatt aacggcctgc
     1861 aagagctgtt tgccgaccgt caatattccc gcgcctggac ggttaaataa ctaaagctgg
     1921 cgcaggcgtt cagctgctgc cagcaacgtc gattccttct tggcaaaaca gagacgaatc
     1981 agtttatgtg ggaagggatc ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc
     2041 tcctgcgtca gccactggca aaactcaaca tcatccaggg tagaaacctc gctgtaatcc
     2101 accagcaaaa agtatgtacc ttcacacggt aaaatctcca gccggctttc atttaaagca
     2161 ttcaccagaa tatcgcgctt ctggcgataa aagtccggta acgcaagata atgctcaggt
     2221 tctgcacgta gcatatcagc aagcgccagc tgtgccgggg tattcaccga aaaggtcaga
     2281 tactgatgta ccttgcgaat ttcggcgctg atgggcgctg gcgcaacaca ataacccact
     2341 ttccagccgg tcatatgata ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc
     2401 agctgcggat gcgccagcac actggcatgg ccctgttgtg aaaagttgat gtgctcgtag
     2461 acttcatcgc taatgacaaa aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa
     2521 tcagcctgct gccagacagt tgcactgggg ttatgcggag tgttgaggat caccagtctg
     2581 gtgcgctcgc ttaataatgc ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc
     2641 agtgccatac gcttcactat tcccccagaa agcgcgatgg cgggggagta actgtcatag
     2701 ctgggatcaa aacaaatcac ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat
     2761 aacgcttccg tcgcccctgc cgttacggtg atatcgctat cggcatctgg ttgatagcca
     2821 tacaaacgtt ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc
     2881 gcgtattggt ttgccccctg ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga
     2941 ccatcaaaat caggaaagcc ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg
     3001 ctcatctggg tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg
     3061 ttatttgtca tttttattgt acctgacgaa gttgcgtgtt gaaggcacta taacacgatg
     3121 ctagtatttg gcaatcaaga cgtttagatg tctaaataaa acaataagga caacacaaca
     3181 tgcctcacaa tcctatccgc gtggtcgtcg gcccggctaa ctacttttca catccaggaa
     3241 gtttcaatca cctgcacgat tttttcactg atgaacaact ttctcgcgcg gtgtggatct
     3301 acggcaaacg cgccattgct gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag
     3361 gggcaaagca tattttgttt cgtggtcatt gcagcgaaag cgatgtacag caactggcgg
     3421 ctgagtccgg tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca
     3481 ccgcgaaagc cctcgcccgc cgtctcggtc tgccgtttgt tgccgttccg acgatcgccg
     3541 ccacctgcgc cgcctggaca ccgctctccg tctggtataa cgatgccgga caggcgctgc
     3601 attatgagat tttcgacgac gccaatttta tggtgctggt ggaaccggag attatcctca
     3661 atgcaccgca acaatatctg ctggcgggga tcggtgacac gctggcgaaa tggtatgaag
     3721 cggtggtgct ggctccgcaa ccagaaacgt tgccgctaac cgtgcgactg gggatcaata
     3781 atgcgcaggc cattcgcgac gtattgttaa acagtagcga acaggcgctg agcgatcagc
     3841 aaaatcaaca gttaacgcaa tcattttgcg atgtggtgga tgctattatt gctggtggtg
     3901 ggatggtcgg tggtctgggc gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata
     3961 acggtctgac tgtcctgccg caaaccgaga aatttctcca cggcaccaaa gtcgcttatg
     4021 gcattctggt acaaagcgcc ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg
     4081 cgtatcagcg ttttcatctg ccgaccacgc tggcggagct ggaagtggat atcaataatc
     4141 atgcggagat cgacaaagtg attacccaca ccctgcgtcc ggtggaatcc attcattacc
     4201 tgccagtcac gctgacacca gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta
     4261 aagcctgacg tgcaaattta gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc
     4321 tgccgctcac ggaaaaactc ttcgtaggtc atcggcgttt gatcgggatg gttaacccgc
     4381 atatgttcga catagttgtc gtaatcaggc ataccaatca tcagcttcgc cgcctgacct
     4441 aaatattttc cggcttttgc cagtgaatca aacatcttgg cctccgctaa ccatgttgct
     4501 gaaagagtgc cggatgcggc gtgaacgcct tatccggcct acaaatgcta tcaacgcgct
     4561 cggtcagtcc cctcgcccca tcggggagag ggttagggtg aggaggaacc ctcggcacta
     4621 tgccaacatt cgccaacatc ccccctcact ctgactttag tgtgcgcctt ttgcctgtgc
     4681 cacgatctcc tcgacatttt ccggcattgg ctcatacggc gtttctttcg ccgttggctt
     4741 cggatctttc aatgccgcca gtgccgtctt aatcgagaac agcgccagaa ccaccacgac
     4801 caccataaag aagatggtta acccggcatc cagacggttg ttgaacacca gttgcgccag
     4861 ctgtgactca gtatactgcg acggaatatt gccgctgtcg atcattgcct ggaacttatt
     4921 agcaatggcc aggaagccga ctttcgcatc cgggctaaac gctttctgcc agcctgcggt
     4981 cagggtacaa atcagcagcc aggccgttgg taccagcgcc acccaggcgt aacgttgacg
     5041 tttcatcttg aacaacacca cggcacagag catcagcgcc atccctgcca gcatctggtt
     5101 ggcaatacca aacagcggcc acagagtgtt aatgccgccc agcggatcga ccacgccctg
     5161 atggaggaag tagccccacg ccagtacgca cagcgctgtt gccagcaggt tagcaggcag
     5221 tgaatcggtc cgcttcaggc ccggagaaac cacgcccagc agatcctgca acataaagcg
     5281 cgcagcacgc gtacccgcat ctaccgccgt cagaataaac agagcttcaa acagaatggc
     5341 gaagtgatac cagaacgcca catccatcat accacccagc gcaccgtgca ggatgtaagc
     5401 catccccacc gccagcgtcg gcgcaccgcc cgcacgggaa atgatcgact gttcacccac
     5461 ttcgctggca atctggttga gcgtatctgg tgtaatgcta aagccccagc tactcaccac
     5521 ctgcgcggca gaagcgacta catccgccgt ccctgccgga gccagcaccg ccattgggct
     5581 gttcatggca aaatacacgc ccggatcgat gatacaggca gaaaccagcg ccataatcgc
     5641 cacgaaggat tccatcaaca tcccaccgta gccgataaag cacgcctgcc cttcgttcgc
     5701 cagcatcttc ggcgtggtgc cggaagagat cagcgcatgg aagccagaca ccgcaccaca
     5761 ggcgatggtg ataaacagga acgggaacag gttaccggtc cataccgggc cagtgccatc
     5821 gacaaatttg gtcagcgcag gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc
     5881 cagaccgacg atagtcccga ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag
     5941 caaccacacc ggcagcaccg ccgccacaaa accgtagccc accagcatcc aggtcagctg
     6001 cacgccggta aagtcaaagt acggtgccca ggtcggactt tctgccaccc agccgccaga
     6061 gataatggcg aaaatcagga acaccaggcc aatcactgac acttcgccaa tgcggcccgg
     6121 acgcagatag cgcaggtaga ttcccataaa aatcgccagc ggaatggtga acgcaacggt
     6181 atacgttccc cacgggctat gagtcagggc tttcaccacg atcatcgcca gtactgcaag
     6241 gataatgacc atgatcataa agcaggctac cagcgcaatc accccggcag ttggccccat
     6301 ctcttctttg accagctcac ccagcgatcg accgtcacgg cgcgtagaaa caaacagcac
     6361 catgaaatcc tgcaccgcac cagcgagaac caccccggcg agcagccaga tcatccctgg
     6421 caggtaaccc atttgcgccg ccagtaccgg cccaaccaac ggacctgctc cggcaatggc
     6481 cgcaaaatgg tgaccgaaca gcactttctt gtccgtcggc acatagtcca gcccgtcgtt
     6541 atggcgcacc gctggcgtca tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata
     6601 cagcccataa aaacggtaag cgatcagata gatacagacc gacgccacca caatccacag
     6661 cgcgttgatc tgttccccac gatttaaagc aatgtatccc agagcaaatg ctcccattac
     6721 agagagcact gtccagacga ggtatttccc tgatttgttc atagttgtta tccgtgtgcg
     6781 tgtccataga gatgttacat tttgtttcta taacatcttt gtaagattta acaacacggt
     6841 aaccatgtaa atcggagttg aaaccaatat ttaacttaac aatgttaact cgatcactcc
     6901 gttaaccgag ttttcctgca atctcattta attctgtcgg gctcatccca aaactgccgt
     6961 acccagccga caagtgcagc aacgccgccc ctgttcatcg aaaacgacga tttcccagct
     7021 ctgattttgc cgcccaagat gcagcggctg gcagacgccg cgtaccttgc cttcagacac
     7081 cgggcgatga tgggtggcgt taagttccgt acccaccaca cactgcccgt cacgggtcat
     7141 cataaatccg gccatcgatc ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag
     7201 caggccaaac ggctgatgag tacgggtatc aaccggcatt tcggcttcca gcacatcatc
     7261 tcccagacgg gtatacacaa ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt
     7321 cagttcgtcg agcgttaaat ggcgtttcca gatcatgctt atgcccccag cgttgagccg
     7381 ccatcgacca caatatcctg tagggtgata tggctggcga ggtcagaggc gaggaacaaa
     7441 atcgtgttgg cgatctcttg tggacgggcg attttcccca gcggaatgcc gagtttaaac
     7501 tgctcgccaa agccgcgaat acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt
     7561 tgcatatcgg tgtcggtgga gccaggcgaa accacattac agcgcacgcc gctacccgcc
     7621 agttccagcc cgacgctcaa cgccaggctt ttcagcgccg ctttcgatgc gccataagca
     7681 ctcataccaa tacgcggcgt gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc
     7741 cgctgacggc gaaactggtt catggtttgc tggaacaggt taaacgcacc gccgacgtta
     7801 accgcaaaag tctgctgcca gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa
     7861 attcccgccg cattgatcag cacgtccagt cgctccgttt cagctaacag tcgctgacat
     7921 acctgcgcga cctgcgcagc atcggcaaca tccatcactt cggtcgcaaa gggatattgc
     7981 tcctgagcga acgcttgatc aaaaccggta actttcgctc ccgcctcaac aaacgccagc
     8041 gccgtggcgt agccgatacc tttacctgcg ccggttaccc agacattttt accgctgaaa
     8101 tccattattt cacctcgcgg gagagtagct tccaccaggc gtcgatggtc gggtttttcg
     8161 ccagcatgac aaagtcgata tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca
     8221 tgcgcaccga atccagaccg tagtcgatca ggttgtcgtc atcgaacggt tcatcggact
     8281 cgtccagcaa cggcaggata acctcacgca gcgccgcttt gctggcgggg acaggtgctg
     8341 gcagtaattc ttcagtcatc accacccggc cagaccgccc ggccacatat ttcagcgaca
     8401 tcaaatgctc gtcacggctg aaatcggcca gcgcatccgc caccataaac ggtttaatat
     8461 cgcgcataaa tgcgtcggtt gcggtggtca tacagccaat gtgggcatat accccggtaa
     8521 taatcagctg gttacgtcca ctctctttga gcatttgctc cagcggagaa cgatgaaacg
     8581 cgctgtagcg ccacttcacc agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca
     8641 ccttttgctg ttccggcgaa cgggtcaggc ccggccccca catatcattc aacagcgccc
     8701 gatcttcatc gctctgctct ttcggctggg cggtgtaata aaccggaata ttgtgctgtt
     8761 tgcagtagtc gcgcagcgca gcaatattcg cgatcacctg ctccatcatc gggcagttct
     8821 cgccccagaa gctgacaaaa tagtcctgca tatcatggat taacaacgcg gcacgttgcg
     8881 gttcaaaggc ccagtcaact ttattcttag ggatatcgtg agactccggc agtgcgtaag
     8941 cctgtaattt tggaatagcc atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc
     9001 cactgacgta attgtttttt atcgactttc ccgaccgccg tcagcggaag tgaatctaca
     9061 cactccacgc gatccggtaa tttaaattcg gcaataccct gttcacgcag gaaacgacgc
     9121 acctgcaccg cgcgcagcgg ctcttttacc accagataag cgcagctttt ttcgcccatc
     9181 agctcatctt ccatgctcac cagtgcggcg tagatcaccg ccgggtggcg cagcagcagg
     9241 ttttcgatct cttcggcagc gatcttctca ccgccacggt tgatctgatc tttctcgcgc
     9301 ccctgcacgg tgatgtaccc ctctggatca atagagatca gatcgccgga acagtaaaaa
     9361 ccgttggcat caaaggcgct ggcattgtgc tgcgggcttt tgtaatagcc acggaaggtg
     9421 tacggaccgc gggtcatcag gcgtccaact tccccttgcg gcagtggatt tccttcggca
     9481 tcggcaaccc acacttcatc gtccggacac attgggtaac cctgggtatg gataattttc
     9541 tccgcgctat catcaagacg ggtgtagttc accagccctt ccgccatgcc aaacacctgc
     9601 tgcaactggc agccaatctc agcgggaata cgcgccgcaa gggtggcaga aagacgtgcg
     9661 ccgccgacct gtaacagttt cagcgaggca agctgcgccc ggctttcacc ttcggtcagc
     9721 gcctgcaacc acaggctgac tgccggtggc accagcgcgg tgacgttcac ctgatgtttt
     9781 tcaatcaatg ggaagcaaag cgtggcgctg ggatcggccg ccagaacaac cgttcctccg
     9841 gcaagaaaga cgcccagcga tcccggcgaa ctcatggcgt agttatgagc cgccgggatc
     9901 gcgcacaggt agcgtgtctg ttgtgtgaac tgacaaatct cgacgctacg acgcacgctg
     9961 tagtagtagt cgttatgagt gcgcgggatc agtttcggtg tgccggtggt gccgccggaa
    10021 agctggaaat aggccacttc atcagcaggt gatggcgtgg cagtaaaatc ctcagccgga
    10081 tggttaatcg catcctgcaa gttatgctca ccgctgtcgt tgtgcagttg caccacgcga
    10141 atggaggaat gttctgtgac gaaagtattg aggaaatcat ccccgctaaa cagcgcatgt
    10201 tggcgatcgg caatcagcaa tgcgggttca atctggctgg cataggcgtt cagttcacta
    10261 cgctgatggc tgaacagcgc cagcaccggc gcaacgccca gtttcagcag cgcgaaaaag
    10321 gtaatataca attcagcgac gttacccagt tgtaccagcg cggtttcacc aggtttaatg
    10381 ccctgacggc gtaaactaca cgcgaggtta tccgccgcct gattcagctc ccggtaactc
    10441 aactgtcgct cgccgtcgat aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg
    10501 tcggtcagcg gcaaatcctg ccagtagcct ttttcccgat agcgacgggc aaactcttcc
    10561 ggccagcggg tgaatggaat gctcatcctc gctccttaat gcaatccaaa aacgttcaac
    10621 atggtagaaa gtttgacgcc tgtttcgcgc cactcaccca acggtgacga cgcaggcaca
    10681 atccccgctc cggcaaacag acgcacctga ttttcccgca gcttcgcgca gcggatggtc
    10741 accacccatt cgccgttacc ttcgctgtca caccaaccca caatgccgcc aaacagttcg
    10801 cggtcgaatg gctccagttc agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc
    10861 agcgcggggg ttggatgcag cagacaggcc agagtcagtg cgttttcttg cgaattcgct
    10921 ttaccttcaa agggagttgc gagatgccac agcgtcggcg tggtaatcaa ttgtggagag
    10981 gagggaacgt gtaactcact actgcgttcg cgcagtacct ctttcatcgc ctgagtcacc
    11041 agttcatgtt catggcgatc tttttctgac gccagcagac gattacccgc ttcgcgatcg
    11101 agcacttcat ccggctgacg acgcgcggaa ccggctaacg gaatggagct aaaacgctcg
    11161 ccgtctttac gtagcagcag ttccgggctg gcccccagca ggacgccacc atcagccagc
    11221 gggacatgga agttgtaact aaccgggttt tgcgcaatca accgttccag caatacgcca
    11281 ctatcaatgg cggcgtcagt ggtgatatca atcaatcgtg acaacaccac tttgtcgacc
    11341 tgcggcgtgg cggtaagtgc ggcggcgcgg gcaaccatct gttcaaacgt ggtttgttca
    11401 ggaattgcct ggcgttccac cacgttcagc gactggctgc gggtaaaacg gcgggctgag
    11461 gtctgttttt cctggcggga gaacgactgc caggattcgg gaatatacag cgacgaaggc
    11521 tgacgtggat cgaagggaat cgccccgacc atcaccggat ttttgatgcc ctgcgctttg
    11581 gcatcggcaa acagcgcggc gagtttttgc tggaagggac tgtcgggcga atccccgttc
    11641 acagccggtt catcgaagcg ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac
    11701 ataaagaaaa agcgattggg cgcaagtgtt gccatggtct gctgtacttc ctcagccagt
    11761 gacgtatcca tatcatcctc cacaaaatga taaaggcttt aataatgatt atcatttata
    11821 ttttcggtcg ctaacctaaa agcaaacgcg cactatgtca actcttgagg taacgcaatc
    11881 tgcaactcgg ggttgcatct ggttgtggtt acaatgaaaa tgagaagcat tattgatgga
    11941 ttcgcataag cgcaatgtga tgtcctgcgc cgttctgccc cctctccctt ccagggtgag
    12001 ggctggggtg agggttaatg ttcgcaccag tgctggctgt tcccctcacc ctctccccaa
    12061 aggggcgagg ggacggattg tgcgctttgt cgaatttgtc attacgccca taaccttatt
    12121 aataacagga agctgatttg tgagactcgc cccgctctac cgcaacgccc ttctattaac
    12181 aggacttttg ctttcaggaa tagccgcagt tcaggccgct gactggccgc gtcagattac
    12241 tgacagccgt ggcacacata cactggaaag ccagccgcag cgtattgttt ccaccagcgt
    12301 caccctgacc ggctcactgc tggcgattga tgctccggtg atcgccagcg gcgcgaccac
    12361 gccgaataac cgcgtcgcgg atgaccaggg ctttttacgc cagtggagca aggtggcgaa
    12421 agaacgcaaa ctgcaacggc tctatatcgg cgaaccgagc accgaagccg ttgccgcgca
    12481 aatgccggat ctgattttaa ttagcgcaac cggcggggat tcggcgctgg cactgtatga
    12541 tcagctttcc accatcgccc cgacattaat catcaattac gacgacaaaa gctggcagtc
    12601 gctgttaacg cagcttggcg aaattaccgg gcatgagaaa caagcggcag agcggattgc
    12661 acagtttgat aagcaactgg cagcggcgaa agagcaaatc aaattaccgc cgcagccggt
    12721 cactgccatt gtctataccg ccgctgcaca cagtgccaat ctctggacgc cggaatcagc
    12781 acaagggcag atgctggaac aactcggctt tacgctggcg aagttgcccg cagggttaaa
    12841 cgccagccaa agccagggca aacgccatga catcattcag cttggtgggg aaaatctggc
    12901 tgctgggtta aatggcgagt cactattcct gttcgccggt gatcagaaag atgccgatgc
    12961 gatttatgcc aatccactgc tcgcgcacct tcccgcagta caaaacaagc aggtttatgc
    13021 gctgggaacc gagacgttcc gtctggatta ctacagcgcc acgcaagtgc tggaacgact
    13081 taaggcgctg ttttaagcat taactgtcag acgctgtcac ctgcggcggc gtctggcgaa
    13141 aacgtcgcaa ctccaccagc accagcaata acaacacgcc gataatcaac aaaccaaaac
    13201 cgcttgcgct tgcggaagca accggtgtca tcatcgcgcc caaaccaccc agcagcgccg
    13261 cgcctatggc atcgcccgtc acgttctgcg ccgtccacaa accgttaatc cgccctaaca
    13321 tcgcttccgg ggtttgcgtt tgcagcattg tatattgcag caacgaacta accgcgctca
    13381 gccagccgaa cagcgccaga caaaccacgc ctaaaatcca catcggcatc aggccaaaca
    13441 gaccaatggc gaggaacgat cccagcgtgg agagcagcat caataaccct ggtcgcgcac
    13501 tatgtgccag cttcccgctg gttaacgcac caatagccgc gccgagcggg atcgccgcgt
    13561 agagaaaacc aatctgtgcc gctgacatct gccagttgtc agccagcgcc ggatacagta
    13621 cccgcaccgc gctcgccatc gtcaataaac cacccagcag cgcaatcccg cccaccagcg
    13681 ggctggcaag cagaaaacga aatcctgcca gtaatgattt caacggatgc tcacgcggct
    13741 gcggtggcgg tggcaacgcc ggaaggctta acaacggtag caaggtaata aacgtgcccg
    13801 ccgccgccag cccgtagttc caggctacgc caccggtcgc cagcaataaa ccgccaatca
    13861 tgggcgaaat caccgacccc agacgcacgg tcaacatggt gatcgccccg gcctgcatta
    13921 agttttcacg ccctaccagt gctggtgtcg ccgccaatag cgccgtaacg ccaagcgatg
    13981 cgaaaaaacc atcccataaa ccaagtaaat agattgccag caatgacggc tccggcagca
    14041 gtgcattaag gcacagtcca atgaagccaa tgccacaggt gccgcgcgcc agcaaaatca
    14101 cttttttgcg ctcatagcga tccgccagca caccgccaac catcaggcca acaaacatcg
    14161 cgccgccggt cagcgtcacc gaaagcccca cctgccaggt agaatgcgtc atcatctgga
    14221 tctgcaccgg caccgcgacg ccgagcaaac ccagagacac aattgagata aaacgagcga
    14281 ggaatactgc gcgaaacgcc gggtgcgttt tcaacaggct gaggttaagc agccaggatt
    14341 gtttattcat tacaatgcct tgccatcaaa tattttaaat acctattctt aggctgcaca
    14401 tgctaacata tccaaataag atagataacg ataattaatt tcattatcat ggaagttcgt
    14461 atgtctggtt ctgttgccgt gacacgcgcc attgccgtgc ccggattgct gttattactg
    14521 attatcgcga cggcattaag cctgctcatt ggggcaaaat cactccccgc ttccgtagtg
    14581 ctggaggccc tctccggcac ctgccagagc gccgactgca ccatcgtgct cgacgcgcga
    14641 ctgccgcgca cccttgccgg tttacttgca ggcggcgcgc ttggccttgc cggggcgttg
    14701 atgcaaaccc tcacccgaaa tccacttgcc gaccccggct tgcttggcgt gaatgccgga
    14761 gccagctttg ccattgtgct gggtgcggcg ctgtttggtt actcatccgc gcaggaacaa
    14821 ctggcgatgg ccttcgccgg ggcgctggtg gcctcattga ttgttgcctt taccggcagt
    14881 cagggcggcg ggcagttaag tccggtgcgt ttaaccctgg cgggcgtggc gctggcggcg
    14941 gtgctggaag ggctgaccag cggcatcgcc ctgcttaatc ctgatgtcta cgatcaactg
    15001 cgtttctggc aagccggttc gctggatatt cgcaatctac ataccttaaa agtggtgctg
    15061 atcccggtgc tgatcgccgg agcaaccgcg ctattactga gccgcgcgct gaacagttta
    15121 agcctcggca gcgacaccgc gacggcgctg ggcagtcgcg tggcgcgcac acagttaatt
    15181 ggtctgctgg cgattaccgt gctttgtggt agtgcgacgg cggtagttgg cccgattgcc
    15241 tttatcggcc tgatgatgcc gcacatggcg cgctggctgg taggtgccga tcatcgctgg
    15301 tcgctgcccg tcacgctact tgctacccct gccctgctgc tgtttgccga tatcatcggg
    15361 cgggtgattg tgcccggcga actgcgcgtt tctgtggtca gtgcgtttat tggtgcaccg
    15421 gtgctgatct tcctcgtgcg acgtaaaacg cgaggtggcg catgatttac gtctctcgcc
    15481 gattaatcat cacctgtttg ctgttggttt ccgcctgtgt ggttgcaggt atctggggat
    15541 tacgcagcgg tgccgtcacg ctggaaacct cgcaggtatt cgccgcgctg atgggcgatg
    15601 cgccgcgcag tatgacgatg gtggtcaccg aatggcgttt accacgcgtg ctgatggcgc
    15661 tgttgattgg cgcggcactg ggcgtcagcg gcgcgatttt tcagtcgctg atgcgtaacc
    15721 cgctcggcag ccctgacgta atgggcttta acaccggggc gtggagcggc gtgctggtag
    15781 cgatggtgct gtttggtcag gacctgacgg ctatcgcgct ggcagcaatg gtgggcggca
    15841 ttatcacttc gctgttggtc tggctgctcg cctggcgtaa cggcatcgac acctttcggt
    15901 tgattattat cggtatcggc gttcgtgcca tgctggtggc ctttaatacc tggctgttgc
    15961 tgaaagcgtc tttagaaacg gcgctaacag cgggtttgtg gaatgcagga tcgctcaacg
    16021 gcctgacgtg ggcaaaaacc tcgccttccg cacccatcat tatattgatg ctcattgccg
    16081 ccgccttact ggtacgccgg atgcgcttgc tggaaatggg cgatgatacc gcgtgtgcgc
    16141 tgggcgtcag cgtcgaacgt tcgcgtctgt taatgatgct ggttgcagtg gtgcttaccg
    16201 ctgcggcaac cgcgcttgcc gggccgattt cctttattgc tttagtcgcg ccgcacattg
    16261 cccgacgcat tagcggcacc gctcgctggg ggctgaccca ggcggcgctg tgcggggcgc
    16321 tgttactgct ggcggccgat ctctgcgccc aacaactgtt tatgccgtat caacttccgg
    16381 ttggcgtcgt taccgtcagc ctcggcggta tttaccttat cgtcttgtta attcaggagt
    16441 ctcgcaaaaa atgaacgaat ctgtagcccg tttgcgcggc gaacagttaa ccctcggata
    16501 tggcaaatat accgttgcgg aaaatctgac tgtagaaata cctgatggtc acttcacggc
    16561 aattatcggg ccaaatggct gcggtaaatc cactttactg cgtactttaa gccgcctgat
    16621 gacgcctgct cacgggcatg tctggctgga tggcgagcac attcaacatt acgccagtaa
    16681 agaggttgca cgccggattg gtctgttggc gcaaaatgct accacgccag gcgatatcac
    16741 cgtgcaggag ctggtggcgc gtgggcgtta cccgcatcaa ccgctattta cccgctggcg
    16801 caaagaagat gaagaagcgg taacgaaagc gatgcaggcc acgggaataa ctcatctggc
    16861 agatcaaagc gtggataccc tttctggcgg acaacgccag cgcgcgtgga tcgcgatggt
    16921 gctggcccag gaaacggcaa ttatgctgct cgacgaaccc acgacctggc tggatatcag
    16981 tcatcagatt gatctactgg aactgttaag cgaactgaac cgcgagaaag gctataccct
    17041 ggcggcggtg ctgcacgatc ttaatcaggc ctgtcgttac gccagccatt tgattgcatt
    17101 gcgggaaggg aaaattgttg ctcagggagc gccgaaggag attgtcactg ctgaactgat
    17161 tgagcgcatt tatggtctgc gatgcatgat cattgacgat ccggtagccg gaacgccgct
    17221 tgtggtgccg ctcggacgaa cggcaccttc aaccgcaaat agttaaacta agtggtctgc
    17281 catcatggca tcctgttttc tggatgccat cgcatagcgc aataacacgc taccacaagc
    17341 caccatcccg ccgatcagcg cggaaaggat cacaataatc gccttacccg gaccgtcttt
    17401 tttcactggc aatgacgggc ttaactgata tttaaacggc gtaaaattca catcgttgat
    17461 atttgctttt gttaactgct caacaagata ctgacgatta cgtaattcac cgttcagttc
    17521 cgcaacgtca gtgaccgctt tttcaatttc cagtttgcgt tcaataccgt ctgcgccgag
    17581 agaaatagag aaatcggggt catctttaac ggcctgacca ttactgtata cgggcttttt
    17641 aattcccgcc gcgttggcaa tgtcgagtga ataattgagg cgctgaatgt ttgcatcaag
    17701 ttgatttttc gttttaatgc gatcctgagc cagtttttct ttttcaaact gggttttgat
    17761 ttccagttta ttacggacgt tttctagtga ctctttcacc accaacgcag agatataatc
    17821 gatatacccg ctcaaaacgg tctgcgcctc ttcactggtt ggcgcggtaa aacttagcgt
    17881 ccaggaggta tacagtgacg gttcatcttt tttcttactg gcattgtcat caaccgcttt
    17941 cattttttcg ctcaatgcaa caatagcgcg atgcaaatcc agttcatcga ttttcgcctc
    18001 ttttaattgg tccatcacat aaggtgatga acgcaggtac tcttccagca agctaaccga
    18061 ctgaaacttc ttgataaaca ggttaaatgc ttctgtacga tcaattttga tatccagatc
    18121 cagcactcga agcttagtga atgttttctc cagctcttgc cactgaacag gttctggagg
    18181 cgtgacaacc gccgcgctgg tccatttttg cggcaggatg aaagagatca gcaagcctgc
    18241 gcaggcaaac gcaaaaatga ccgccatgac cgtttttttg gcccgccata aaactgcgat
    18301 tagattaagt aaatcaattt cattattact gggcgacgcc agaggatact caggaaaatg
    18361 agcgtcactt ccctgtttaa tattcagtga tgacatgggg tataacctga aaatgagtcc
    18421 aataggggaa atctccacgg cgctcacttt accagttggt gataattatc cgaagctgaa
    18481 gtttgtaaat tccttccact gaatgcaac
//
LOCUS       DAZFRJ010000080        18301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000080 DAZFRJ010000000
VERSION     DAZFRJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.080"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..57
                     /gene="tnaC"
                     /locus_tag="ACHKUN_002535"
     CDS             <1..57
                     /gene="tnaC"
                     /locus_tag="ACHKUN_002535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO6660775.1"
                     /translation="CVTSKWFNIDNKIVDHRP"
     gene            278..1693
                     /gene="tnaA"
                     /locus_tag="ACHKUN_002536"
     CDS             278..1693
                     /gene="tnaA"
                     /locus_tag="ACHKUN_002536"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO6660776.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1784..3031
                     /gene="tnaB"
                     /locus_tag="ACHKUN_002537"
     CDS             1784..3031
                     /gene="tnaB"
                     /locus_tag="ACHKUN_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO6660777.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3163..4338
                     /gene="mdtL"
                     /locus_tag="ACHKUN_002538"
     CDS             3163..4338
                     /gene="mdtL"
                     /locus_tag="ACHKUN_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO6660778.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4313..5272
                     /gene="yidZ"
                     /locus_tag="ACHKUN_002539"
     CDS             4313..5272
                     /gene="yidZ"
                     /locus_tag="ACHKUN_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO6660779.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5429..6178
                     /gene="yieE"
                     /locus_tag="ACHKUN_002540"
     CDS             5429..6178
                     /gene="yieE"
                     /locus_tag="ACHKUN_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO6660780.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6200..6766
                     /gene="yieF"
                     /locus_tag="ACHKUN_002541"
                     /gene_synonym="chrR"
     CDS             6200..6766
                     /gene="yieF"
                     /locus_tag="ACHKUN_002541"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO6660781.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6820..8157)
                     /gene="adeP"
                     /locus_tag="ACHKUN_002542"
     CDS             complement(6820..8157)
                     /gene="adeP"
                     /locus_tag="ACHKUN_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO6660782.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8324..8989
                     /gene="yieH"
                     /locus_tag="ACHKUN_002543"
     CDS             8324..8989
                     /gene="yieH"
                     /locus_tag="ACHKUN_002543"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO6660783.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9056..9529
                     /locus_tag="ACHKUN_002544"
     CDS             9056..9529
                     /locus_tag="ACHKUN_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660784.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9578..10165
                     /gene="cbrC"
                     /locus_tag="ACHKUN_002545"
     CDS             9578..10165
                     /gene="cbrC"
                     /locus_tag="ACHKUN_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO6660785.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10227..10949)
                     /gene="yieK"
                     /locus_tag="ACHKUN_002546"
     CDS             complement(10227..10949)
                     /gene="yieK"
                     /locus_tag="ACHKUN_002546"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6660786.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(10964..12133)
                     /gene="yieL"
                     /locus_tag="ACHKUN_002547"
     CDS             complement(10964..12133)
                     /gene="yieL"
                     /locus_tag="ACHKUN_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6660787.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12160..13776)
                     /gene="bglH"
                     /locus_tag="ACHKUN_002548"
     CDS             complement(12160..13776)
                     /gene="bglH"
                     /locus_tag="ACHKUN_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO6660788.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13862..15256)
                     /gene="bglB"
                     /locus_tag="ACHKUN_002549"
     CDS             complement(13862..15256)
                     /gene="bglB"
                     /locus_tag="ACHKUN_002549"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO6660789.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15275..17152)
                     /gene="bglF"
                     /locus_tag="ACHKUN_002550"
     CDS             complement(15275..17152)
                     /gene="bglF"
                     /locus_tag="ACHKUN_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO6660790.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17286..18122)
                     /gene="bglG"
                     /locus_tag="ACHKUN_002551"
     CDS             complement(17286..18122)
                     /gene="bglG"
                     /locus_tag="ACHKUN_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO6660791.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4625 a         4563 c         4320 g         4793 t
ORIGIN      
        1 tgtgtgacct caaaatggtt caatatcgac aataagattg tcgatcaccg cccttaattt
       61 acccttctgt agccatcacc agagccaaac cgattagatt caatgtgaac gattagtttg
      121 ctatatctta attttgcctt ttgcaaaggc catctctcgt ttatttactt attttagtaa
      181 atgatggtgc ttgtatatat acatggcgaa ttaatggata ttgcagatgt aatattcaca
      241 gggatcactg taattaaaat aaatgaagga ttatgtaatg gaaaacttta aacatctccc
      301 tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt accactcgcg cttatcgtga
      361 agaggcaatt attaaatccg gtatgaaccc attcctgctg gatagcgaag atgtgtttat
      421 cgatttactg accgacagcg gcaccggggc ggtgacgcag agtatgcagg ccgcgatgat
      481 gcgcggcgac gaagcctaca gcggcagccg cagctactat gcgttagccg agtcagtgaa
      541 aaatatcttt ggttatcaat acaccattcc gactcaccag ggccgtggcg cagagcaaat
      601 ttatattccg gtactgatta aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat
      661 ggtggcattc tctaactatt tctttgatac cacgcagggc catagccaga ttaacggctg
      721 taccgtgcgt aacgtctata tcaaagaagc cttcgatact ggcgtgcgtt acgactttaa
      781 aggtaacttt gaccttgagg gattagaacg cggtattgaa gaagttggcc cgaataacgt
      841 gccgtatatc gttgcaacca tcaccagtaa ctctgcaggt ggtcagccgg tttcactggc
      901 aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat attccggtgg taatggactc
      961 cgcacgcttt gctgaaaacg cctatttcat caagcagcgt gaagcagaat acaaagactg
     1021 gaccatcgag cagatcaccc gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc
     1081 caagaaagat gcgatggtac cgatgggcgg cttgctgtgc atgaaagacg acagcttctt
     1141 tgatgtgtac accgagtgca gaaccctttg cgtggtgcag gaagggttcc cgacatatgg
     1201 cggcctggaa ggcggcgcga tggagcgtct ggcggtaggt ctgtatgacg gcatgagtct
     1261 cgactggctg gcttatcgta tcgcgcaggt acagtatctg gtcgatggtc tggaagagat
     1321 tggcgttgtc tgccagcagg cgggcggtca cgcggcattc gttgatgccg gtaaactgct
     1381 gccgcatatc ccggcagacc agttcccggc acaggcgctg gcgtgcgagc tgtataaagt
     1441 cgccggtatc cgtgcggtag aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg
     1501 taaacaactg ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg caacatatac
     1561 tcaaacacat atggacttca ttattgaagc ctttaaacat gtgaaagaga acgcggcgaa
     1621 tattaaagga ttaaccttta cctacgaacc aaaagtattg cgtcacttca ccgcaaaact
     1681 gaaagaagtt taattaatac tacagagtgg ctataaggat gttagccact ctcttaccct
     1741 acatcctcaa taacaaaaat agccttcctc taaaggtggc atcatgactg atcaagctga
     1801 aaaaaagcac tctgcatttt ggggtgttat ggttatagca ggtacagtaa ttggtggagg
     1861 tatgtttgct ttacctgttg atcttgccgg tgcctggttt ttctggggtg cctttatcct
     1921 tatcattgcc tggttttcaa tgcttcattc cgggttattg ttattagaag caaatttaaa
     1981 ttatcctgtc ggctccagtt ttaacaccat caccaaagat ttaatcggta acacctggaa
     2041 cattatcagc ggtattaccg ttgccttcgt tctctatatc ctcacttatg cctatatctc
     2101 tgctaatggt gcgatcatta gtgaaacgat atcaatgaat ttgggctatc acgctaatcc
     2161 acgtattgtc gggatctgca cagccatttt cgttgccagc gtattgtgga taagttcgtt
     2221 agccgccagt cgcattacct cattgttcct cgggctgaag attatctcct ttgtgatcgt
     2281 gtttggttct ttcttcttcc aggtcgatta ctccatcctg cgcgatgcca ccagcaccac
     2341 tgcgggaacg tcttacttcc cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt
     2401 tggtttccac ggcaatattc ccagcctgat tatttgctat ggaaaacgca aagataagtt
     2461 aatcaaaagc gtggtatttg gttcgctgct ggcgctggtg atttatctct tctggctcta
     2521 ttgcacgatg gggaatattc cgcgcgaaag ctttaaggcg ataatctcct caggcggcaa
     2581 cgttgattcg ctggtgaaat cgttcctcgg caccaaacag cacggcatta tcgagttttg
     2641 cctgctggtg ttctctaact tagctgttgc cagttcgttc tttggtgtca cgctggggtt
     2701 gttcgattat ctggcggacc tgtttaagat tgataactcc cacggcgggc gtttcaaaac
     2761 cgtgctgtta accttcctgc cacctgcgtt gttgtatctg atcttcccga acggctttat
     2821 ttacgggatc ggcggtgccg ggctgtgcgc caccatctgg gcggtcatta ttcccgcagt
     2881 gcttgcaatc aaagctcgca agaagtttcc caatcagatg ttcacggtct ggggcggcaa
     2941 tcttattccg gcgattgtca ttctctttgg tataaccgtg attttgtgct ggttcggcaa
     3001 cgtctttaac gtgttaccta aatttggcta aatccttcaa gaagccagcc attcgctggc
     3061 ttcttgcctc tcaggaaatc acttatgtcc aaatggcaac tcgcctgatt ctccttcacc
     3121 acgtatgctt tgcgtcacct tactatcagg acgctttagc ccatgtcccg ctttttaatt
     3181 tgtagttttg ccctggtttt actttatccc gccgggattg atatgtacct cgttggttta
     3241 ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt tgcatattgc gttctccgtt
     3301 tatctggcgg gcatggcagc tgcgatgtta tttgccggta aagtggccga tcgttcaggg
     3361 agaaagcctg tcgccatccc cggcgcggcg ctatttatta tcgcttcggt gttctgttca
     3421 ctggctgaaa ccagcacgtt atttcttgca gggcgctttc tacaggggtt gggcgcaggc
     3481 tgttgttacg tggtggcgtt cgccattttg cgcgacacgc tggatgatcg acgtcgggct
     3541 aaagtgctgt cgttactcaa cggtattacc tgcatcattc cggtgttagc gccggtgctc
     3601 ggacacctga ttatgcttaa attcccttgg cagagtctgt tctggacgat ggcaatgatg
     3661 ggcatcgcgg tactgatgtt atctttgttt attttaaaag aaacgcgccc agcgtccccc
     3721 gctgcttcgg acaaaccacg agaaaatagc gagtcgctgc ttaatcggtt tttcctcagc
     3781 cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca ctttcgtcaa tacatcgccg
     3841 gtattgctga tggaaatcat gggttttgag cgcggagaat acgccaccat tatggcgttg
     3901 accgctggcg tcagcatgac cgtttcattc tccacgccat ttgcgctggg aatttttaag
     3961 ccacgtacgt tgatgatcac ctcgcaggtg ttattccttg cggcggggat cacccttgcc
     4021 gtttcacctt cccatgcggt ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg
     4081 gtaggttttg gcgtagcgat gagtcaggcg ttaggaccat tttcattacg cgcgggcgta
     4141 gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt cactgtggat ttggctggca
     4201 gcggtggttg gtatcggcgc atggaatatg ctgatcggga ttctgattgc ctgtagcata
     4261 gtgagcctgt tactgattat gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa
     4321 atccatcacc acgcttgatc tcaatctgct gctctgtctg caactgctga tgcaggagcg
     4381 cagcgtgacc aaagcggcga agcggatgaa cgtgacccct tcagcggtga gtaagtcgct
     4441 ggcaaagtta agagcgtggt ttgacgaccc gctctttgtg aactcaccgc tggggctgtc
     4501 gcccacaccg ctaatggtca gcatggagca aaatctggcg gaatggatgc aaatgagcaa
     4561 tctgctgctg gataaaccgc accaccagac accgcgcggc ctgaagtttg agttggcggc
     4621 ggaatcgccg ctgatgatga tcatgcttaa tgcgctgtcg aaacggatct accaacgtta
     4681 cccgcaggcg accatcaaac tacgtaactg ggattacgat tccttagatg ccattactcg
     4741 tggtgaagtg gatattggtt tttccggtcg cgaaagccat cctcgctcgc gggagctgtt
     4801 aagctcgcta ccgttagcca ttgattatga agtgctgttt agtgatgtac cctgcgtctg
     4861 gttacgcaaa gatcatccgg cactgcatga aacgtggaat ctggacacct ttttacgtta
     4921 tccgcatatc agcatttgct gggaacagag cgacacctgg gcgctggaca atgtgttaca
     4981 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaatttgagc agtcactgtt
     5041 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
     5101 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
     5161 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
     5221 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
     5281 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
     5341 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttatattca
     5401 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
     5461 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
     5521 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
     5581 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
     5641 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
     5701 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
     5761 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
     5821 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt
     5881 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     5941 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     6001 cgtgctagtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     6061 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     6121 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     6181 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     6241 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     6301 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     6361 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     6421 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     6481 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     6541 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     6601 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     6661 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     6721 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     6781 agcaatcatg catggcgggt ttttaacgcg ctatcgattt tagtgagcgt cgataaagac
     6841 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggcttagat cacgcagacg
     6901 tccggtaccg attttcatca cgcagtagga gataaagccc agcgcgatac cttcggtaat
     6961 cgagaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     7021 ctgccagttc acgcgtgcca gactggaagt catcagcacg ccaacgtaaa tcagcgcacc
     7081 agctgcagcg tagcctggca ccatccccgc cagcggcgac agaaagataa ccagcaggaa
     7141 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     7201 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     7261 actgtcgaca tacagcgcct gcttcatgcg cgggaattta cccttctcat ccgccaggcc
     7321 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaggttga ccagcatgaa
     7381 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     7441 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     7501 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     7561 ggccagaata gcaatgatga agaagccgag gatacccaga agtacgctgt gagaagtcag
     7621 attaccgatg ctcaccagcg tttccgggtt agcgacaatc acacctgcgt ttttcagccc
     7681 catcatgcca atgaacagac cgataccgct ggtaataccc acacgcagac tcaccggaat
     7741 gttggcaatc atccagtagc gaacgcggaa aatcgtcagt aacagcagac ctatcgcgcc
     7801 ccagaagatt gcgcccatcc cgacctgcca cggcaagccc atcgcctgta caacgacaaa
     7861 agcgaagaac gcattcaggc ccatagcggg tgccagtgca actggcaggt tagcaaacag
     7921 tcccatcata atactgccga atgcagcgat cagacaggta gtgacgaaga cggcgctggt
     7981 atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag
     8041 gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt
     8101 aaacacgcgt tccagcatcc cctggccaga agcctgggtt gtgtgttgtt gactcattat
     8161 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatga gacaatatgg
     8221 acggctgaag ataacttttt ttcattgtat ttgcttatac ggcaacgatt gcgtcatgcg
     8281 aattggcatg acgaaaacgt taaactaacg ataaaggaaa gacatgtccc agatagaagc
     8341 ggtatttttc gactgcgacg gtacgctggt cgacagtgaa gtcatttgct cccgcgcata
     8401 tgtaacgatg tttcaggaat ttggtattac gctcgatcct gaagaggtat tcaaacgttt
     8461 caaaggtgta aaactgtacg aaattatcga tattgtttcc cttgaacatg gtgttacgtt
     8521 agcgaaaaca gaagctgaac acgtttaccg tgcagaagtc gctcggctgt tcgattcaga
     8581 actggaagcc atcgaagggg ctggagcgct cctgtcagcg atcactgcgc caatgtgtgt
     8641 ggtatctaac ggcccaaata acaaaatgca gcattctatg ggcaagctga atatgttgca
     8701 ctacttcccg gataaactgt tcagcggcta cgatattcag cgctggaagc cagacccggc
     8761 gttaatgttc catgcggcaa aagcgatgaa tgtaaatgta gaaaactgca ttctggttga
     8821 tgactcagtt gccggtgcgc aatctggtat cgacgcaggt atggaagtgt tctacttctg
     8881 cgccgacccg cacaataagc cgatcgttca cccgaaagtc accaccttta cccatctttc
     8941 gcagttaccg gaactgtgga aagcgcgtgg ttgggatatt acggcatagt tcttcacact
     9001 cccttcactt accccgctta aattggcgct caaaggtaag taaagggagt ttgatatgtc
     9061 tgtttcacgt cgggtaatac atcacggact ttattttgcg gttttaggac cgttaattgg
     9121 tgttctgttt cttgtcctct acatattctt cgcaaaagaa ccgctggttc ttttgcgaag
     9181 aatacaggta cttccattgt ttctcttatt gtcgattact acgggagtta ttcctgcatt
     9241 gttaaccggc gtgatggtcg cctgtctgcc ggaaaagatc gggtcacaga aaaattatcg
     9301 ttgtctggct ggtggcatag gtggcgtcgt tatcaccgag atctattgtg cagttattgt
     9361 acatattaag ggcatggctt cctcggagtt gtatgaaaac attctttctg gtgacagtct
     9421 cgttgtccgc atcattcctg cattgctggc aggtgtggtg atgagcagaa tcattacccg
     9481 tctgcccgga ttggatattt catgtcctga aacagactct ttaagttaag cgggatactt
     9541 tatctttggg ctactcaaaa gcagacagga tgttactatg actcaaaata tcaggccgtt
     9601 accccaattc aaatatcatc ccaagccact ggaaacaggc gcatttgaac aggataaaac
     9661 cgtagagtgc gattgctgtg aacaacagac gtcagtttat tactcgggtc ccttttattg
     9721 cgttgatgaa gttgaacatc tctgtccgtg gtgtattgcg gacggttctg ctgctgaaaa
     9781 gtttgcaggt agttttcagg atgatgccag catagaaggt gttgaatttg agtatgatga
     9841 agaggacgaa tttgccggta ttaagaacac atatcctgat gaaatgctga aagagttggt
     9901 tgaacgcacg ccaggttatc atggatggca acaggaattc tggcttgcgc attgtggcga
     9961 tttctgtgct tttatcggct atgtgggctg gaatgatata aaagatcgcc tcgatgaatt
    10021 tgccaacctt gaagaagatt gtgagaattt cggtattaga aattctgatc tagctaaatg
    10081 cctgcaaaag ggtggtgatt gtcagggtta tctcttccgc tgtctccact gcggcaagct
    10141 gagactgtgg ggtgattttt cgtagttatt taaataatga gaacaggccg gagcgtaatt
    10201 catacatccg gccttatttc ttcagcttaa tcgagcgcta attctgcggc agcggcttta
    10261 tcagcgatta ccatcagcga aggatgcagc tgcaacacgg aagctggaac atcttcggta
    10321 accggacctt gcagcacatt tttcagtgcc tgcgctttac ctgcgccact gacaattatc
    10381 agcaagtttt tcgccgccat gatgcttttc ggccccatcg tcacgtagct gtcaggcact
    10441 aacgaaaagt cgccgcccaa ctcaccatgc gccacaatat caaccatctc tccctggatc
    10501 gggaactcca cggtttgctc atggaaatgg gtcgtattcg gcaaattgcc acaaaaatga
    10561 ccatctgcac ctaatcccag caccaccaaa tccaggccgc cttcacgcgc cagtttctga
    10621 tcatgctcgc ggtagttatc aatagtgagc ttctggatat tctcttcttt gatccccgca
    10681 ggggtgaaaa acagattacg cagattggta atcgttacac cttctccctc tttgccgcga
    10741 aagggaattt catcgaaatt atagaaatag cagttatcat accagggctt acctttaacc
    10801 agggtggtga ggtattcata catgcctttg ggcgtgctac cggcggtaat tgccaggtta
    10861 acacgacgcg tcttcgacat ataaccaagc agatggtgtg ccgcgacacg gctcatttcc
    10921 tggtaatctt cggtaatgat taatttcatc ttagtgcctt attttattta aataatttct
    10981 gtacgaaggc tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt
    11041 cctggtaatc aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct
    11101 tgccggttac gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa
    11161 tagctgccgg atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa
    11221 caccactgaa tgtggccagc cagccaaagc tttccagatg attcattccg gaaaccagcg
    11281 cctggtaccc gccttgtgaa agccctgcca gcgcgcgacc atcggcatct ttacggacat
    11341 taaaacgctt gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag
    11401 cattcagcgg ataaaagact ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc
    11461 ccttcgcatc ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag
    11521 ccagcaggtt atccatgatt tgcgggatac gcccctgatc gatagcggaa cgtccggtat
    11581 caccaaaacc gtgatagaaa tagagcactg gcaaaggctc gcccatgccg gtgtaacctg
    11641 gcggggtcca gacatacatc tgacgttcag attgcaatgc gttggagtgg taagttatgg
    11701 cgatcaaatc accatgcgca acagaacgcg tatccagata actgcctggc accagaatca
    11761 tactggagtt aacctggcgc tgaggcttag tcattgcggt gcctgtatca atgctgcgta
    11821 caccatcaac attgaaaaaa tactcgtaca ggttgccttt caggataggt gtgcgccacg
    11881 accagactcc tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca
    11941 ccactacaga gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg
    12001 cattgacctg agtcacatac tgtttcaccg ggatagtggg tgccgggctg gctggcatat
    12061 ccgctgctat ggcccactga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa
    12121 tttttatatt catcgtaata ttcctttcat taagttccat taccaccaga tttcagcctg
    12181 ggcaccgaca gcgaactggt cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt
    12241 ttccagggct ttgatatacg tggcgtagaa acgaatttcc ggacgcgagg tcaacatact
    12301 ggtattgact ttaaaggtat ggaagagcgt ggttttataa ccagactcat taaatttatt
    12361 actattcgca tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata
    12421 ttggtcccaa atataggccg ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc
    12481 gcctgtttcg tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat
    12541 aaaatgatcg ccgatatagg cttcgccctg tgaagtcaga cgaactgctg ttccgccggt
    12601 gtgatcacca taataacgac cattaaaggt ggtaaatgga cttgcgccag cataacggcc
    12661 aaagttactg gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccttt
    12721 atcaaatttc tgcgttaaag atgtgccaaa catccaggta tctttccagt cataatagcc
    12781 gttattatcc tgattatctt tttccgatgc gctttcgttt gccgtgacat aacgaccact
    12841 caccattaag gtcgctttat cccataacgg gatatcttta tagcgtaaat caatagtatt
    12901 ggtattaatc tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac
    12961 cagcgcgata tcaattttac ccggaccgac tttccagttt tccagaccta cacccgctgc
    13021 ggcatcagta cgctgcgttt tccagtccag catctggatt tcaattttcg gcgcaccgtg
    13081 tttacccacc cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacgta
    13141 catatcggag aactgcataa agttctcgcc accggcgtta tcgccaaacc agccggtaga
    13201 gtactgctga ccaacgttac catccatcat cacaacggca tcaacccgtt tgccgttttc
    13261 gttgtagaca cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg
    13321 gcccagagaa ccaatcgccc atgatttagg tgaaccatga gaggcggtcc cccaaccaga
    13381 acggtaatag ccgttgtaac taaagccaat ttcatctttc acaaacttac tgaaatcttt
    13441 cagagtcatg gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttgac
    13501 cagtacagca tcaggttttg cggcactact ggtcgggaac ggattggcgg catacccttg
    13561 atcattcgta cttacgctac gattcaccgt cgctggcgta tattttttct tctcttcatc
    13621 tttatacttt ttgagttcgc tttgcgtttc tcttaacgct ttttctaata actcaaggcg
    13681 atgttccacc gttaatgatt cagccaatga ttgtgcagaa aaggctaacg gtgccattaa
    13741 taagatggca gaggtaataa gatttcgtct aaacataata tccctttatg gtgcaaagaa
    13801 agaattaacg tatcgcatcc agactgttct gaatgcgacg ataattaagg tgctttattg
    13861 gttatttttt taatgacagc ccccgcgttt tgatcacttc tgcataccat ccgaagcttt
    13921 tcttacgcgt tcttgtgagg cttccttcgc cattatcatc acgatccaca taaataaagc
    13981 cgtagcgctt agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg
    14041 tgtaccccat aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt
    14101 ttaaataggc aattcgataa tcgtcctgta tcgaaccatc cgcttcaacg ctgtcttttg
    14161 cgcctaatcc gttctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta
    14221 acagaacccg taatccaacc gaatcaattt gccaccccca ctctgaactt ttcagatgcg
    14281 gattggggat catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg
    14341 aaacacaacc agtcatgtaa taactaaaag agatgaaatc gacggtatgt tttaaatctt
    14401 ctgcgtcact ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat
    14461 agccgggata ctggccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt
    14521 ccatggcctg caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa
    14581 gcatattgcc gattttcgct tcggggagca ggctatgaca ggctttaact gcccgcgcac
    14641 tggcaaccag ttgatggtgg atagcctgat aaacttccgc ctcgccactc tcttctgcca
    14701 gccccacgcc cgtgaatggc tcgtgtaacg acatgttgat ttcattaaac gtcagccata
    14761 acgccacttt atgttggtag cgagtaaaga ccgtgcgggc gtaatgctcg aagtgatcga
    14821 tgaccgctcg attagcccaa ccgccgtagt ttttcaccag cccatatggc atttcgtaat
    14881 gggataacgt taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgatcgt
    14941 aaaacgctaa ccccgcttca ttcggttcga cttcgtcgcc ctgagggaaa attcgcgccc
    15001 aggcaatgga aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg
    15061 ggtaacggtg ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg
    15121 gttccatttt tcccattacg ccatgaggct gtaaatctga ggtcgagatc cctttgccat
    15181 cttcctgcca ggcaccttcc acctgattgg cagctgttgc gccaccccaa agaaatgttt
    15241 ctggaaatgc tttcataatt aactcctttt atcgttagcg aatgatggat aacagcggtt
    15301 cacctgcgct tatctgcgcc gtgccgtggg gtaatacgtc cgtaaaatca tcgctattac
    15361 tgattaatac cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag
    15421 aaatcagccg atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt
    15481 tgccgtccag ttttacggtg tcgataccga catgaatcag gagctccaca ccatcatctg
    15541 actcaatgcc aatggcgtgt aatgtggcga acaacgaagc aattcgaccc gcaaccggag
    15601 aacgcacttc accaaccgag ggcagaatgg cgataccttt acccaacagg ccactggcaa
    15661 acgtggtatc agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac
    15721 cgccctgctc aggtggtgta ataacctctg gtgttttctc ttgcggggca ccctgcgctg
    15781 gctgacgttt agcggtgatg aaatgaagca tcaccgtacc gacaaatgcg caaccgatgg
    15841 caatgacacc gccaataacg ctggcccaga cggtgaaatc aattcccgtt gacgggatgg
    15901 tttgcatgaa ggtgaaaata cttggcaaac caaaggagta cactttcgtt tgcgcgtagc
    15961 caatgatggt ggcccccaaa gccccactga tacaggcgat aacaaagggg tacttacgcg
    16021 gcagattaac gccatatacc gctggttcgg tgataccaaa caggcccgtc aacgccgctg
    16081 atcccgccac cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc
    16141 cgacctgcgc cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg
    16201 tgaagttatt gatacacagc ggcaccaggc cccagtgcag tccgaacatg acgaagattt
    16261 gccagaagcc gcccattacc gcgcctgcaa atgcaggaac cgcctgataa agccagagat
    16321 aaccggcggc aatcagttcg cttatccagg ttgatagcgg ccccaccagc agaaaggtga
    16381 cgggtgtgat aaccatcaga catagcaatg gtgtgaagaa atttttgatt gccgacggta
    16441 accacgcatt aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa
    16501 taaccgatga cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccagct
    16561 catccgcttt ttgcccgttc tcgaaagcag tcagaattaa tggatgcact aacgctccac
    16621 caatcaccat ggcagtaaat ggattaccgc cgaagcgttt ccccgcggtg tatcccagga
    16681 ttatcgggaa gaaccaaaac aaggcatcac tggcgctgaa taaaattaaa taagtaccac
    16741 tttgttcggt cgtccactga aaagtgagcg ccagagccag catacctttc aggatcccgg
    16801 ttgccgccat caaaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt
    16861 ttagcagatt acctttctca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg
    16921 ccacgctgtt gaccgccagg aagacatcgg ccacatggtt acctatgacc acctgaaact
    16981 gaccaccgct ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg
    17041 ctttgctttc atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa
    17101 tgttatctgc gcccccgact cctgcgacta tttttctggc taactccgtc ataacttgcc
    17161 ctctaccgct ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc tgacgtgaat
    17221 caagcaattt ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata
    17281 atatttcagt gttctttgcg cacgcgctct atatttatgg ctaaaaacat aatctctgcg
    17341 ggtgaaattt tacgttgata ctgcaaacca ataaaaatgg cgatccgttc cgcacattgc
    17401 catgcttgcg ggtaattttg ttttactgct tgttgtaatg attcatcact atcgttaatt
    17461 gaagcatgtt caagaatacg ccaggataaa aacttcagat gtgtaaccag tcgctgataa
    17521 ctcaagcttt cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg
    17581 cgcattaact gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc
    17641 agatgcatgg caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca
    17701 ataatggtta acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc
    17761 cacagcaacg ggttgggcag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa
    17821 tggtcagtta gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa
    17881 gagataatac gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt
    17941 aatcgcccgt tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta
    18001 attctttcgc cagcgcgttt ttgaaagcca attcctcgcc ccatgacgac tttttcccgc
    18061 tgttgatcat caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc
    18121 atagcaagga ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg
    18181 tcaggttttg cctgcgaatg cagtaacaat ccagtcattt attaatggtt tttataacga
    18241 acatccaggt tcggaaatta atttaattgc gtgcagagaa aatagcaatg cgctattgat
    18301 a
//
LOCUS       DAZFRJ010000081        18275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000081 DAZFRJ010000000
VERSION     DAZFRJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.081"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..236
                     /locus_tag="ACHKUN_002552"
     CDS             <1..236
                     /locus_tag="ACHKUN_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6660792.1"
                     /translation="FGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVG
                     LLHELCDKLKTRIKTAQQTQLHLADALTDAAIN"
     gene            457..798
                     /gene="symE"
                     /locus_tag="ACHKUN_002553"
     CDS             457..798
                     /gene="symE"
                     /locus_tag="ACHKUN_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO6660793.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(845..2008)
                     /locus_tag="ACHKUN_002554"
     CDS             complement(845..2008)
                     /locus_tag="ACHKUN_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO6660794.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(2056..2938)
                     /locus_tag="ACHKUN_002555"
                     /pseudo
     CDS             complement(2056..2938)
                     /locus_tag="ACHKUN_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(3035..3199)
                     /gene="yjiS"
                     /locus_tag="ACHKUN_002556"
     CDS             complement(3035..3199)
                     /gene="yjiS"
                     /locus_tag="ACHKUN_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO6660795.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3376..4788
                     /gene="yjiR"
                     /locus_tag="ACHKUN_002557"
     CDS             3376..4788
                     /gene="yjiR"
                     /locus_tag="ACHKUN_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6660796.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(5030..5974)
                     /locus_tag="ACHKUN_002558"
     CDS             complement(5030..5974)
                     /locus_tag="ACHKUN_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6660797.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            6441..7673
                     /gene="mdtM"
                     /locus_tag="ACHKUN_002559"
     CDS             6441..7673
                     /gene="mdtM"
                     /locus_tag="ACHKUN_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO6660798.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            7714..8994
                     /gene="yjiN"
                     /locus_tag="ACHKUN_002560"
     CDS             7714..8994
                     /gene="yjiN"
                     /locus_tag="ACHKUN_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO6660799.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            9110..10261
                     /gene="yjiM"
                     /locus_tag="ACHKUN_002561"
     CDS             9110..10261
                     /gene="yjiM"
                     /locus_tag="ACHKUN_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO6660800.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            10271..11038
                     /gene="yjiL"
                     /locus_tag="ACHKUN_002562"
     CDS             10271..11038
                     /gene="yjiL"
                     /locus_tag="ACHKUN_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO6660801.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            11035..11292
                     /gene="ytiA"
                     /locus_tag="ACHKUN_002563"
     CDS             11035..11292
                     /gene="ytiA"
                     /locus_tag="ACHKUN_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO6660802.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            11357..12217
                     /gene="yjiK"
                     /locus_tag="ACHKUN_002564"
                     /pseudo
     CDS             11357..12217
                     /gene="yjiK"
                     /locus_tag="ACHKUN_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            12285..13463
                     /gene="yjiJ"
                     /locus_tag="ACHKUN_002565"
     CDS             12285..13463
                     /gene="yjiJ"
                     /locus_tag="ACHKUN_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660803.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13476..14030)
                     /gene="kptA"
                     /locus_tag="ACHKUN_002566"
     CDS             complement(13476..14030)
                     /gene="kptA"
                     /locus_tag="ACHKUN_002566"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO6660804.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            14280..14963
                     /gene="yjiH"
                     /locus_tag="ACHKUN_002567"
     CDS             14280..14963
                     /gene="yjiH"
                     /locus_tag="ACHKUN_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO6660805.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14960..15421
                     /gene="yjiG"
                     /locus_tag="ACHKUN_002568"
     CDS             14960..15421
                     /gene="yjiG"
                     /locus_tag="ACHKUN_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO6660806.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15434..16606
                     /gene="iadA"
                     /locus_tag="ACHKUN_002569"
     CDS             15434..16606
                     /gene="iadA"
                     /locus_tag="ACHKUN_002569"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6660807.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16671..17583
                     /gene="hypT"
                     /locus_tag="ACHKUN_002570"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             16671..17583
                     /gene="hypT"
                     /locus_tag="ACHKUN_002570"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(17576..17968)
                     /gene="iraD"
                     /locus_tag="ACHKUN_002571"
     CDS             complement(17576..17968)
                     /gene="iraD"
                     /locus_tag="ACHKUN_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO6660808.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(17965..18048)
                     /gene="idlP"
                     /locus_tag="ACHKUN_002572"
     CDS             complement(17965..18048)
                     /gene="idlP"
                     /locus_tag="ACHKUN_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO6660809.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(18164..>18275)
                     /gene="ytiD"
                     /locus_tag="ACHKUN_002573"
     CDS             complement(18164..>18275)
                     /gene="ytiD"
                     /locus_tag="ACHKUN_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO6660810.1"
                     /translation="PELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         4305 a         4626 c         4534 g         4810 t
ORIGIN      
        1 catttgggcg ctcacagatt taccgctttg ataatggctc agcacaaccc aacctatcag
       61 caaatagtgt aatgttgtat tcattcccat gcccaccatt aactgaacaa tatagaatat
      121 tttcacaagt aggtttactt catgaactat gcgataagct taaaactcgt ataaaaacag
      181 cccaacaaac ccaactgcac cttgcagacg cactcactga cgcggcgata aactaatttt
      241 ctcgccctca aaaaaccaat aaagcggcta aatggccgct ttattcacat caccaaaaat
      301 tatatttcac gctaattttc atcttatttt cattaatttt tagctggact ttccctgtat
      361 ttactgatga tatatccagg tatttagcgc ggtgcggatg tgcgccaaca cacccgcacc
      421 gctaatcaca atcactatta tcggagaata gcagttatga ctgacacgca ttctattgca
      481 caaccgttcg aagcagaagt ctccccggca aataaccgtc aattaaccgt gagttatgcg
      541 agtcgctacc cggattacag ccgtattccc gccatcaccc tgaaaggtca gtggctggaa
      601 gccgccggtt ttgccaccgg cacggcggta gatgtcaaag taatggaagg ctgtattgtc
      661 ctcactgccc aaccacccac cgccgaagag agcgaactga tgcagtcgct gcgccaggtg
      721 tgcaagctgt cggcgcgtaa acaaaagcag gtgcaggatt ttattggggt aattaccggt
      781 aaacagaaag tcgcctgata aattgacccg gtagcgccag tgctaccggg tattattcgc
      841 cgcgttatac caacgcgctc tccatctgtt tttcaaatgc ctgacgaatt aatcccataa
      901 tgtaaggaag ttgtgcgagg tcgctgaaac caatttctac atcgccatta ccccagcgcc
      961 caacatttgt cacatctttg gcaatacctt tcggatcgac cagttcatga aactgcatat
     1021 taagcgacaa ccgcagtcgg cttttttgcg gtacaacatc aacaaaattc gtttcagctt
     1081 taaacgcaat atagagcttc aatacttcct gcgtgatccc tgcgtccagg cgcataactt
     1141 catcgcgaag atgatcgaat aacacccggc tatgcgaacc atcagcaaga aaaggataat
     1201 cactcaggct gtaactggtt ttgttttcag gctgcccccg ataaacagct aaaacctctt
     1261 cagaaagaga aggcgcttgc cagacctgaa ccgcgagatc ggccagacga tcggcacggg
     1321 cgcgaatggc ggcttcatcc catttttcgc actgcccaag accgatattc aaatagagcg
     1381 ggctatgttt aaagccgtct tcaatatcgc gtttttccgc gaaaggtctg tcactgtagc
     1441 gggagttata gcgcgttaaa gtgagattcc ccaacgtatg taacagttct ttatgcacac
     1501 gctgccagtc gcttcccagc tcttcacgcc atttagccga cagattttcg ttctgcggca
     1561 taatgtgctc aatcgtaaac tcttccacgc gctctctgcg tttatcgttt tccagtcttc
     1621 gtaaccagta gctgcggctg cggaagttat agagatcgcg aacttttaat tcccgtttaa
     1681 attcatcatc gttggggaaa cgacgatatg aaggcaggtt cataaaatgt acctgaatac
     1741 tttccagata tttttcttta ttaatgacct tataaaaagt ggcaaacgtc ttgttcaaag
     1801 aattcgtcgg aattgcacat actgcacggc ggaaaacata agattcaatt aaacgaatta
     1861 tgctcaggaa atcttcgtga gacaaatcgt catttttata gtcatgataa agcgccagta
     1921 agaaaggata cgccacatca acctttaact cgcgcaaatc ctgaaaagcc gtagcaagcg
     1981 atttgtcact ttcttttccc aatgccattg cacagtaata ctccgcgtag atatgaatat
     2041 cttcaaccag ccgatctacg cctttttcag caacactctg gctgcgggca tggagtttaa
     2101 atgcctcata gacttcatct gtccgaggga tctcccccgt ttttaccgtc agataatgac
     2161 gcataaaact gtcaaaatat tcgctgtaac cttgctgacc aaaggcgact tccattggac
     2221 gccagtgatc ttcatacaac cgggtttgat gctctggttc gaggcccatc agaataaaat
     2281 tgcgcaccag atcggcctga cttaacgcct taccggtgga gttcatactt tcaaaaatca
     2341 gttgcggatt atcctgacca cgattaagcg ccacatcgac aattaataac tttgctaacc
     2401 cacgacataa ggggatcaag tcatcaccga gtttggcaat ttgttcatca aagaaagtaa
     2461 agttttccat tattcgatgg gaatagtttt ctggcattgg tctttgtttt attaacgcca
     2521 gtaaactgtc tttatcagtc tcggtcagta gtaatttaaa acctttctcg ccggactcat
     2581 aaggattgag caaataataa ttacgcaatt tcattgctga aaagccatca aaaacttcgt
     2641 cttcgccaag atggcgcgat aaagcctcaa tcagcaacat tgcggtcgtc agccgttgtt
     2701 gaccatcaat gaccagtaac ggagaaatac cagaaacctg atacaacccc tgctcaatat
     2761 aaacaaccga accgataaaa tgcgctgata tatcgtcacg ctttccggca cgaatgatgt
     2821 cgtcccaaag ttgccgacat tgttgttctg tccatgaata agtccgttga taaatgggaa
     2881 taacaaactg ctgcgaacgt tttaaaaaat cgagtaggcg ggcttctgta gctttcattc
     2941 tgacgtcttt ccgtgaagcg atgatttcgc aataataacc acttcctttt ggaggttata
     3001 gagaaatcct gtttatccgc gatgaattgc cccctcactc cacatcctcc ctgcgtaacc
     3061 caatatctct caaccgctca tcactcatct gctgtaacac tcgtcgggtc tgcatttgcc
     3121 gccgccaacg cctcaccgcc tgccagagtt gcacaagtct gataaacggc gttttggctt
     3181 tgttttcgtg aaattccatc tgctgcctcc tcatctttca gaggcttcat catccacctt
     3241 ctggcagttc acaatacaga tgcaaatatt agttttattt aacatacaga caggttaaaa
     3301 cagcatctgc atccggtttt ttagcctcat ctgtaccggt ttttctttct gtatggtcca
     3361 aataaggaat acagcatgac gcgttatcaa catctggcga ctctacttgc cgagcggatt
     3421 gagcaagggc tgtatcgtca tggggagaaa ttgccgtcgg tgcgtagctt aagtcgggag
     3481 cacggcgtca gcatcagcac cgtgcagcag gcgtatcaga cgctggagac gatgaagctc
     3541 ataaccccac agccgcgttc gggttatttt gtcgcacaac gtaaagccca gccgccagtg
     3601 ccgccgatga cgcgtccggt gcagcgcccg gtggaaatta cccagtggga tcaggtgctg
     3661 gatatgctgg tggcgcatag cgacagttcc attgttccgt taagcaaaag cacgccggat
     3721 gtcgaaacgc ccagcctgaa accgctctgg cgggagctaa gccgggtggt gcagcataat
     3781 ctgcaaaccg tgctcggtta tgacttgtta gccggtcagc gggtattgag agagcagatt
     3841 gcccgcctga tgctcgacag cggctcggtg gtcaccgccg atgacatcat tatcaccagc
     3901 ggctgccata actcgatgtc gctggcgtta atggcggtgt gtaaaccggg cgatattgtc
     3961 gcggtcgaat ctccctgtta ttacggttcg atgcagatgc tgcgcggcat gggcgtgaaa
     4021 gtgattgaaa tcccaaccga tccagaaaca ggcatcagcg ttgaagcgct ggaactggcg
     4081 ctggaacagt ggccgattaa aggcatcatt ctggtgccaa actgtaataa tccgctggga
     4141 tttattatgc cggacgcacg caaacgggcc gttctctctc tcgctcagcg tcatgatatt
     4201 gtgatttttg aagatgatgt ctacggcgaa ctggcaacgg agtatccgcg cccgcggacc
     4261 atccattcat gggatatcga cgggcgagtg ctgttgtgca gctcgttcag taaaagtatt
     4321 gctccaggcc tgcgcgtggg ttggggcgca ccggggcgtt atcacgataa actgatgcat
     4381 atgaaatacg ccatcagcag ctttaatgtg ccgtccacgc aaatggcggc ggcaacgttt
     4441 gtgctggaag gtcactatca tcgccatatc cggcggatgc ggcagaacta tcagcgcaat
     4501 ttggcgcttt atacctgctg gatacgggaa tattttccct gcgaaatctg tattacgcgc
     4561 ccgaaaggcg gatttttact gtggatagaa ttgcctgaac aggtcgatat ggtctgcgtt
     4621 gcgcggcagc tgtgccgcat gaatatccag gtggcagcag gctcgatttt ctcggcttcc
     4681 ggcaaatacc gtaattgtct acgcatcaac tgcgctttgc cgctcagcga aacctatcgc
     4741 gaagcactaa agcaaattgg cgaggccgtg tatcgggcaa tggaataaat tcgaattagt
     4801 ggcgaagcac ctgatttcct ctcacgaatc gcctgtaact cgttgtatct ttgcagggtc
     4861 atcgccacgt tccagcatgg cttaggcaaa gtgtgcttcg ttcataccgg atgcggcgtg
     4921 aacgccttat ccggcctaca aaccgcgcaa attcaataga ttgcagagga aatgtaggtc
     4981 tgataagcgt agcgcatcag gcagctttgc gtttgtcatc cgtctctgat tagtattcga
     5041 tccatggatc gcgcttgatt tcaggcaagg gctgctctaa tgcctgcatt tgctctgtcg
     5101 aaagtccggt aatcttttgt atccattcag gatctgcccc attctgcaac aacaatctaa
     5161 ggaggcgttg ttccccttct tctttcccct tttccattcc caacagccat ccatcattat
     5221 aaattcgctc tgcaatcgtc attattcgct ccctgtgttg tggcattcga cgggtaagtt
     5281 cgctgataaa cttcttaaaa cgcgcttcat cgccagtcag taaaatgtaa tttaacagcg
     5341 cagttatctg gctgtcatta gcacactctg taaccagtaa tactaccagt tggtcgataa
     5401 gccccatcag atcgcgctgg cgaatatgct tttggatcaa ctccaacagg gcgactctgc
     5461 gatgctgcac aatctcgtcg tctggcacga cagtgatatc caccagcggg aacgcggcgg
     5521 tataaagttt ccgtgcggta gttgggtcgg caaattcgtc cagccagcac agggaccagg
     5581 gataaggact acggctaccg tgataaaata gcatcggtat aaccagcggt agcgcccgac
     5641 gtttatcatg ctcgatatgg cgctgcatca ccgccatgga atagcgcatc aggcgaaagg
     5701 ccatatgaat atcctcacgg ctctgatgtt caatcaccac ataaatatag ccatcgcctt
     5761 cgcgggtatt taccgaccac agaatatcgg agtgtagcgc ccgcaatttt tcatcgacaa
     5821 agctggcgga ttccagtttt aagctgtcga gatcgcacag ttcacgtaaa tctttgggta
     5881 aatagatctc cataaaatcc cgcgcggtgt cagggtgggt gagaaaggat ttaaataatg
     5941 cgtcatgcgg cgtgctggtt gtgaagtttg tcatggcgat ccgtcacctg ttgataagat
     6001 ggcccgactt tagcgactaa caaaaccatt cacacctggc aatttcccgt tccccgaggc
     6061 gctttccagg ctatttttgt tcctctgcaa gcgatacatt tttccggaaa caggctcgcg
     6121 ttttacattt cctgtacccg gtagatattc ccctttccac cgcgctcttt tgattaatcg
     6181 caaattttaa ttacacttaa ggtgtatatt ttctatgcaa accatcaatt caagaggtgt
     6241 aatgtgctga tgactatttg taatcgttat acattctgac ccgaactcag aaggtatttc
     6301 tctgtctgta taagcacagg cagtgtggtt gattacatga aattcagtac atttgtcctt
     6361 ctcgcctacc tttcgtcatt accgacggta ttgaatttcg ttttccccgt tggggttctc
     6421 cggacaagga gttgtttgtt atgccacgtt tttttgcccg ccatgccgcc acgctgtttt
     6481 tcccgatggc gttgattttg tatgattttg ccgcgtacct gtcgacggac ctgattcagc
     6541 cggggatcat taatgtggtc cgtgatttta atgccgatgt cagtctggcc ccggcagccg
     6601 tcagtctcta tctcgcgggc ggtatggcgt tacagtggct gctggggccg ctttccgaca
     6661 ggattggccg caggccggtg ctgattaccg gggcgctaat atttaccctt gcctgcgctg
     6721 cgacaatgtt cacaacgtct atgacacagt ttcttatcgc gcgtgcaatt cagggcacca
     6781 gtatctgttt tattgctacc gttggttatg tcacggtgca ggaggcgttc ggacagacaa
     6841 aagggatcaa gttgatggcg attatcacct ccatcgtact gattgcgccg attattggcc
     6901 cgctttccgg cgcagctctg atgcactttg tgcactggaa agtccttttt gccatcattg
     6961 cggttatggg ttttatctca tttgttggct tactgctggc gatgccagag acggtgaagc
     7021 gcggcgcggt tccatttagc gccaaaagcg tcttgcgcga ttttcgtaat gtcttttgca
     7081 atcggctgtt cctctttggc gcagcaacca tctctttaag ctatatccca atgatgagtt
     7141 gggtggctgt ctcgccggtg atcctgattg acgcaggcgg cttaacaact tcgcagttcg
     7201 cctggacaca ggttccggtg ttcggcgcgg tgattgtcgc gaatgccatc gtggcgcgtt
     7261 tcgttaaaga tccgaccgaa ccgcgcttta tctggcgtgc cgtacccatt caactggtcg
     7321 gcctcgcact gttgattatc ggcaatctgc tgtcaccgca cgtctggctg tggtcggtgc
     7381 tgggcaccag tctgtatgct ttcgggattg gtttaatctt cccgacctta ttccgcttta
     7441 cacttttttc caataactta ccgaaaggga ccgtctccgc atcgctgaat atggtgatcc
     7501 tgatggtgat gtctgtctcg gtcgaaatcg gccgctggct gtggtttaac ggcggtcgct
     7561 tgccgtttca tctgttagcc gtcgtagcgg gagttatcgt cgttttcacc ctggcggggt
     7621 tgctcaatcg cgtgcaccaa catcaggctg ccgagctggc ggaggagcgg tgatttttgc
     7681 gcgatcctgc cgtcaggctc tattcttaac gttatgaata aactcattga actcagacgc
     7741 gccaaaatgt tggcgctctc tttactgctt atcgccgctg ctacctttgt cgttacgctg
     7801 tttttgccac ccaatttttg ggtgagcggc gtgaaggcaa ttgctgaagc ggcgatggtc
     7861 ggcgcgctgg cggactggtt tgcggtggtg gcgctgtttc gccgcgtgcc aattccgatc
     7921 atttctcgtc atacggcgat tatcccgcgt aataaagacc ggattggcga aaatctcggt
     7981 cagttcgtgc aggaaaaatt tctcgatacc cagtcgctgg tggcattgat tcgacgtcac
     8041 gaaccggcgt tgctaattgg taactggttt agccagccag aaaacgcccg ccgcgttggt
     8101 cagcatctgt tgcagatcat gagtggtttt cttgaactga ccgatgatgc acgtattcag
     8161 cgcctgctta agcgcgcggt ccatcgggcg attgataagg tcgatctttc cggaaccagt
     8221 gcgttgatgc tggagagtat gaccaaaaac gatcgtcatc aggtgctgct cgatacgctg
     8281 atcgcacagt tgattgctct tctccagcgc gataaatcgc gcaagtttat cgcccaacaa
     8341 attgttcgct ggctggagag tgagcatcca ctgaaagcca aaattctccc caccgaatgg
     8401 ctgggcgaac atagcgcgga gttggtttct gacgcggtga attctttgct tgatgatatc
     8461 agccgcgatc gtgcgcatca gatccgccat gcatttgatc gcgccacttt tgccctgatc
     8521 gacaagttga aaaacgatcc ggaaatggca gcgcgagctg atgccgtaaa aagctatctg
     8581 aaagaggatg aagcctttaa ccgctatctc agtgaattgt ggggggattt acgggaatgg
     8641 ctgaaagcgg atatcaacag tgaagattct cgtgtgaaag aacgtatcgc gcgggcgggt
     8701 caatggtttg gcgaaacgtt aattgccgat gatgccttgc gggcgtcgtt aaatggtcat
     8761 ctggaacaag ccgcgcaccg cgtcgcacct gagttttccg cattcctgac gcgccacatc
     8821 agcgatacgg taaaaagctg ggatgcccgg gatatgtcgc ggcaaataga gttaaatatc
     8881 ggcaaagatc tgcagtttat ccgtgtcaac ggtacgctgg ttggcggttg tattgggcta
     8941 attttatatt tgctgtcaca gctcccggcc ttgttccccc tcggcaattt ttagaaatcc
     9001 atgaaaaact aagtggctaa ccgcgagaga gatcaaataa accacactaa aagaatggta
     9061 ataatgtggc ctcttttcat tatctcccgt ggtacgggga aggaaaatca tgtcacttgt
     9121 caccgatcta cccgccattt tcgatcagtt ctctgaagct cgccagaaag gctttctcac
     9181 cgtcatggat ctcaaggagc gcggcattcc gctggttggc acttactgca cttttatgcc
     9241 gcaagagatc ccgatggcag ccggtgcggt tgtggtttcg ctctgttcca cctctgatga
     9301 aaccattgaa gaagcggaga aagatctgcc gcgcaacctc tgcccgctga ttaaaagcag
     9361 ctacggcttc ggtaaaaccg ataaatgccc ctacttctac ttttctgatc tggtggtcgg
     9421 tgaaaccacc tgcgacggca aaaagaaaat gtatgaatac atggcggagt ttaagcctgt
     9481 tcatgtgatg cagctgccaa atagcgttaa agacgatgcc tcgcgtgcgt tatggaaagc
     9541 cgagatgctg cgcttacaaa aagcggtgga agaacgtttt gggcacgaaa ttagcgaaga
     9601 tgctctgcgc gatgccattg ctctgaaaaa ccgcgaacgt cgcgcactgg ctaattttta
     9661 tcatcttggg cagttaaatc ctccggcgct tagcggcagc gacattctga aagtggttta
     9721 cggcgcaacc ttccggttcg ataaagaggc gttgatcaat gaactggacg cgatgaccgc
     9781 ccgcgttcgt cagcagtggg aagaaggcca gcgactggac ccgcgtccac gcattttaat
     9841 caccggctgc ccgattggcg gcgcagcaga aaaagtggtg cgcgcgattg aagagaatgg
     9901 cggctgggtt gtcggttatg aaaactgcac cggggcgaaa gcgaccgagc aatgcgtggc
     9961 agaaacgggc gatgtctacg acgcgctggc ggataaatat ctggcgattg gctgctcctg
    10021 tgtttcgccg aacgatcagc gcctgcaaat gctcagccag atggtggaag aatatcaggt
    10081 cgatggcgta gttgatgtga ttttgcaggc gtgccatacc tacgcggtgg aatcgctggc
    10141 gattaaacgt catgtgcgtc agcagcacaa cattccttat atcgctattg aaacagacta
    10201 ctccacctca gatgtcgggc agctcagtac ccgtgtcgcg gcctttattg agatgctgta
    10261 aggagtggca gtgacatatt cgattggcat tgattctggt tctaccgcca ccaaagggat
    10321 cttactggca gacggcgtga ttacgcgccg tttcctcgtt ccaacaccct ttcgtccggc
    10381 aacagcaatt actgaagcct gggaaactct gcgcgaaggg ttagagacaa cgccgtttct
    10441 gacgctcacc ggctatggac ggcaactggt ggattttgcc gataaacaag tgacggaaat
    10501 ctcctgtcat gggctgggcg cacggtttct tgcgccagca acgcgcgcgg taatcgacat
    10561 cggtggtcag gacagcaaag tgattcagct tgatgatgac ggtaacctgt gcgatttcct
    10621 gatgaatgac aaatgcgcgg cgggcaccgg gcgtttcctg gaggtgatct cgcgcacgct
    10681 tggcaccagc gtcgagcaac tcgacagcat taccgaaaat gtcacgccgc acgccatcac
    10741 gagtatgtgc acagtgtttg ctgaatcaga agcgatcagc ctgcgctcag cgggcgtcgc
    10801 gccagaagcg attctcgccg gagtgattaa cgcgatggcg cggaggagtg ccaatttcat
    10861 tgctcgtctc tcctgtgaag cgccgattct gtttactggt ggcgttagtc attgccagac
    10921 gtttgcccgg atgctggaat ctcatctggg aatgccggta aatacccatc ctgatgcgca
    10981 atttgctggc gcaattggcg cggcggtaat tggtcaacga gtgaggacac gccgatgaaa
    11041 gagtttttat ttttatttca ctccacagtc ggcgtcatac aaacccgcaa agcgttgcag
    11101 gcagcgggca tgacttttcg cgtcagcgat attccccgtg atttacgcgg cggctgcggg
    11161 ttatgcattt ggctgacctg tccccccggc gaggaaatgc aatgggtgat ccctgggctg
    11221 actgagtcaa tttattgtca gcaggatggt gtctggcgct gcattgcaca ttaccgggtt
    11281 tctccgcgtt aaggattcat taaggtcgct acgtatactt ctcgatatct tattgatttg
    11341 ccttaaaggg attaccgtga caaaaagtat ttcgttaagt aaacgcatct ccgttattgt
    11401 gattcttttt gccattgtgg cagtgtgtac tttcttcgtg caatcttgcg cccgtaaaag
    11461 caatcatgca gcataatttc agaattacca cgccaccatt gacggcaaag aaattgcagg
    11521 cataacgaat aatatctctt cgctgacctg gtcggcacaa agcaatactc tttttagcac
    11581 tatcaacaaa cctgccgcca ttgttgaaat gaccaccaat ggtgatttta tccgcacaat
    11641 tcctctggat tttgttaagg atctcgagac gatcgaatac attggcgata atcagtttgt
    11701 cattagcgat gagcgcgatt acgccattta tgtgatttca ctgaccccaa actcggaagt
    11761 aaaaatcctc aaaaaaataa aaatcccgct gcaagactcc ccaaccaatt gcggttttga
    11821 agggttggct tattctcgcc aggatcacac tttctggttt tttaaagaga aaaatccgat
    11881 tgaggtctac aaagttaacg ggctgttaag tagcaatgaa ttacacatca gcaaagacga
    11941 ggcactacaa cggcaattta ctttagatga tgtgtccggc gcagaattta accagcaaaa
    12001 aaatacgctt ctggtgctgt ctcatgaatc tcgcgcactc caggaagtaa ccctggttgg
    12061 cgaggttatc ggtgaaatat cactaaccaa aggtagtcga ggtttgtcgc ataacatcaa
    12121 acaggccgaa gggattgcga tggatgcctc tggtaatatt tatatcgtta gcgaaccaaa
    12181 tcgtttttat cgctttaccc ctaaatcatc gcattaacag gctggcatat ttcacctttt
    12241 tgttattaac ttgttaacaa caaaaatgac atacaaggtt aaacatgcct tcgtcctcgc
    12301 atcccgtaga acgcttttct ttcagcaccg cgcttttcgg gatgctggtt ctgaccttag
    12361 gtatgggatt aggtcgcttc ctttatacgc ctatgttgcc cgtcatgatg gcggaagggg
    12421 cgttttcatt tagccagctc tcgtggattg ccagcggcaa ctatgcgggg tatctggctg
    12481 gcagtctgct attttcgttt ggcgcatttc accagccgtc gcgcctgcgc ccatttctgt
    12541 tggtttctgc cctggcgagc gggttgttga tcctcgcaat ggcatggttg ccgccattta
    12601 ttctggtgtt attgattcgc ttcctggcgg gtgtcgccag cgccggtatg ctgatttttg
    12661 gttcgacgct gattatgcag cacacgcgcc atccttttgt gctggcagct ttgttttctg
    12721 gcgttggcat tggcattgca ctgggcaacg aatatgttct ggcaggcctg cattttgcct
    12781 tatcctcgca aacgttatgg caaggcgccg gggcgctttc tggcatgatg ctgattgcac
    12841 ttacgctttt aatgccctcg aaaaaacacg ccatcgcacc aatgccattg gcaaaaacgg
    12901 agcaacagat aatgagctgg tggttactgg ctattctgta cggcctggcg ggatttggtt
    12961 atatcatcgt cgcgacttac ctgccgctca tggcgaaaga tgcaggctca ccattgttaa
    13021 ccgcccatct ctggacgtta gtcggcttat caattgtgcc aggctgcttt ggctggctat
    13081 gggcagcaaa acgttggggg gctttgcctt gcctgacggc gaatttgctg gtgcaggcta
    13141 tcagtgtgct gcttactctc gccagcgact ctcctctctt gcttatcatt agtagtatcg
    13201 gttttggcgg tacctttatg ggcacgacat ctctggtgat gactatcgcc cgccaactga
    13261 gcgttccggg aaatctcaat cttttaggct ttgtgacgct catttacggc attggacaaa
    13321 tcctcggccc ggcgctgacc agtatgctcg gcaatggaac atcggcgctt gccggcgcca
    13381 ctctctgcgg cgcggcggcg ttgtttattg ctgcgcttat cagcacagta caattattta
    13441 agctgcaagt ggtcacttca tgaggagtgt ttcccttacc actcaagaaa ttcgaccgca
    13501 accgttgatg tcagccagac cccgttttcc gtttgccaga aagggatgcc tcgtttcgcc
    13561 atttcctgtg ctttgacggt taagataacc ggcgatccgt gtcttgctcc cactttgcgt
    13621 gccgttgctt catcagcaga gagatgtacg tagtgacgct cacctgcaat cagcccctgt
    13681 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt aaagaaactg tggcggtgtt
    13741 ttttcggcaa aggaaatagc gacctgcgaa gtcaaatgcc cctgaaccgc gcggatgcac
    13801 ctaccatcac tggaataact aaaacgcttt ttatcactgg tggcgaccac ggtatccagc
    13861 aatgtacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt atcgatatcc
    13921 gcccatcctt cacggtcaag tacaatacca atcgcctccg gtttgtgtcg gaggacaaaa
    13981 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat ctcgttttcg
    14041 tccctggatt ttctgctttt atttactgat gtcattgtca ctgggtttat gaattttctg
    14101 caactcaatc ctgcgcagag ccttttccac acagcgtaat ttttccgcta tgcaaaaaac
    14161 gcatagccag aaacatactt gcattttccc catacccaca tggccgataa ggtttttcag
    14221 ctccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
    14281 tgacacaaca aggggatgct gtagccgggg atctggcgac agaaaaagtg ggcattaaag
    14341 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc gggacagaca
    14401 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
    14461 cgaacggagc caccacctca tttcgcgggg cgggtggcgc gggggctaaa gatggctttc
    14521 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
    14581 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
    14641 tgggcattcc agggatctgc tcgctggcgc tgatcgctaa cctgcaaaac actgatgccg
    14701 ccgccggtat gaccaaagag ctggcgcagg aaggggaaat taccgaacgc gataaagtga
    14761 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
    14821 tcgcggtgtt cgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
    14881 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
    14941 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
    15001 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
    15061 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
    15121 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
    15181 cgcattaatg agtatgggtg gcgcagtcgg tgttgccgcc agtctggcaa ccgctggcgc
    15241 attaaccggt cacgatgtca ccgttttact gcccgcgatg tatttaatgg gcaacccggt
    15301 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
    15361 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
    15421 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
    15481 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
    15541 cgccgttgcc agcaatatcc cttctgacat tgtaccggac tgcacggttg tcgatctcag
    15601 cgggcagatc ctctttcctg gttttattga tcaacacgtc catttgattg gtggtggcgg
    15661 tgaagcaggt cccacgacgc gcacgccaga agtggcgcta agtcgcctga cggaagcggg
    15721 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
    15781 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
    15841 ttatcatgtc ccttcccgca ccattacagg ttccgtggaa aaagacgtgg cgattatcga
    15901 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
    15961 tcacctggct aatatggcgg cggaatcccg cgttggcggt ttgctcggcg gtaaacctgg
    16021 cgtcaccgtg ttccacatgg gcgacagcaa aaaggcgtta cagcctgtct atgacctgct
    16081 ggaaaactgc gatgtgccga tcagcaagtt gctgccgacc cacgttaacc gcaacgttcc
    16141 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc actatcgata tcaccagcag
    16201 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
    16261 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
    16321 agggaattta acccatatcg gtgttgccgg ttttgaaacg ttgctggaaa ccgtgcaggt
    16381 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
    16441 agccggtttc cttaacctga ccgggaaagg cgaaattctg ccaggcaatg atgctgactt
    16501 gctggtcatg acgccagaac tgcgcattga gcaggtatac gctcgcggca aactgatggt
    16561 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
    16621 cccgaatgga cgtagaatgc ccgcaagggc ggctgacaga gtaaaacgta atggatgact
    16681 gtggtgcgat tttgcataat attgaaacca aatggcttta tgattttctg accctggaaa
    16741 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
    16801 gccgcatccg tgcgctggaa caggcgattg gtgttgaatt gtttaaccgc caggtaacgc
    16861 cgctgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
    16921 agttagagag caatctggca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
    16981 agatagccgc tgcacactct ctttccctcg ggctgttacc gtccattatc agccagatgc
    17041 cgccgctctt tacctgggca attgaagcta ttgatgtcga tgaagcggtc gataaactgc
    17101 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
    17161 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
    17221 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
    17281 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
    17341 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
    17401 ttgcctggct gccggaatac gctatccaac aagaaattcg cagcgggcag ctcgttgtgc
    17461 tgaatcggga cgaactggtg atcccgatcc aggatctacg cataccggat gaatactcgc
    17521 atgaatcccg ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc
    17581 tgacattctc cagcatcgca ctacaccaac cgccattatc ccagaccaga tcaaacgtta
    17641 gcggttcacc gtcgtggtac agcattatct caaggctgat cgccagagtg tggcagtacg
    17701 cttctttaaa attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac
    17761 gcagctgacg gcaataccag ctctggagat gtttgtcgct ctggttatgc acttgccact
    17821 cgggcatgcc tgaaacaagt aatgacggta atgacgaatg aggagaatta aaaagagtga
    17881 ggagatcgct gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta
    17941 aaacagcctg acgtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc
    18001 ggcttgccct gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt
    18061 ttgttcatta catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa
    18121 ccttacttgc ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa
    18181 tcatcaaact caaaaaaaaa ccgctagtat attctgaata ttccgaatag ttacgtagat
    18241 gaaaatactt gtcaccaccg ctacttaatt ccggt
//
LOCUS       DAZFRJ010000082        18169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000082 DAZFRJ010000000
VERSION     DAZFRJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.082"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..740)
                     /gene="aroD"
                     /locus_tag="ACHKUN_002574"
     CDS             complement(<1..740)
                     /gene="aroD"
                     /locus_tag="ACHKUN_002574"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO6660811.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLT"
     gene            complement(771..1637)
                     /gene="ydiB"
                     /locus_tag="ACHKUN_002575"
     CDS             complement(771..1637)
                     /gene="ydiB"
                     /locus_tag="ACHKUN_002575"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO6660812.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1649..2914)
                     /locus_tag="ACHKUN_002576"
     CDS             complement(1649..2914)
                     /locus_tag="ACHKUN_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660813.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3141..4355)
                     /locus_tag="ACHKUN_002577"
     CDS             complement(3141..4355)
                     /locus_tag="ACHKUN_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660814.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4455..4811)
                     /locus_tag="ACHKUN_002578"
     CDS             complement(4455..4811)
                     /locus_tag="ACHKUN_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO6660815.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4947..5054)
                     /gene="rprA"
                     /locus_tag="ACHKUN_002579"
     ncRNA           complement(4947..5054)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKUN_002579"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5240..6352)
                     /gene="ydiK"
                     /locus_tag="ACHKUN_002580"
     CDS             complement(5240..6352)
                     /gene="ydiK"
                     /locus_tag="ACHKUN_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO6660816.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6741..9797
                     /locus_tag="ACHKUN_002581"
     CDS             6741..9797
                     /locus_tag="ACHKUN_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO6660817.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9794..10204
                     /gene="menI"
                     /locus_tag="ACHKUN_002582"
     CDS             9794..10204
                     /gene="menI"
                     /locus_tag="ACHKUN_002582"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO6660818.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10304..10492
                     /locus_tag="ACHKUN_002583"
     CDS             10304..10492
                     /locus_tag="ACHKUN_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO6660819.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11040..11408
                     /gene="sufA"
                     /locus_tag="ACHKUN_002584"
     CDS             11040..11408
                     /gene="sufA"
                     /locus_tag="ACHKUN_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO6660820.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11417..12904
                     /gene="sufB"
                     /locus_tag="ACHKUN_002585"
     CDS             11417..12904
                     /gene="sufB"
                     /locus_tag="ACHKUN_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO6660821.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12914..13660
                     /gene="sufC"
                     /locus_tag="ACHKUN_002586"
     CDS             12914..13660
                     /gene="sufC"
                     /locus_tag="ACHKUN_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO6660822.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13635..14906
                     /gene="sufD"
                     /locus_tag="ACHKUN_002587"
     CDS             13635..14906
                     /gene="sufD"
                     /locus_tag="ACHKUN_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO6660823.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14903..16123
                     /gene="sufS"
                     /locus_tag="ACHKUN_002588"
     CDS             14903..16123
                     /gene="sufS"
                     /locus_tag="ACHKUN_002588"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO6660824.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16136..16552
                     /gene="sufE"
                     /locus_tag="ACHKUN_002589"
     CDS             16136..16552
                     /gene="sufE"
                     /locus_tag="ACHKUN_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO6660825.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16701..17705
                     /gene="ldtE"
                     /locus_tag="ACHKUN_002590"
     CDS             16701..17705
                     /gene="ldtE"
                     /locus_tag="ACHKUN_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO6660826.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17768..18004)
                     /gene="lpp"
                     /locus_tag="ACHKUN_002591"
     CDS             complement(17768..18004)
                     /gene="lpp"
                     /locus_tag="ACHKUN_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO6660827.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4758 a         4398 c         4679 g         4334 t
ORIGIN      
        1 gttaataccg tgcgtaaatc atttaccgag atttgccctg gtgcagaggc tttttttacc
       61 gcaccaaaag ttgccgccga gccaaatact tcaccagcca gacgagaaat tacgccagtt
      121 tttgccatcg acatcgtgat gattggacga tcggcatact gctcctgcat ctccagggtc
      181 gcggtaagca acgtcagcac atcgctggta ctttgcggca tcagcgcaat cttaggaata
      241 tcggcgtcga aggactgcat tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt
      301 ttatggaagt catggttgga catgacaact ttcacatcat gcgcgtgggc gtaggcgacg
      361 gtttctttaa cctgatcatc gccggtaaat aactccagat cgatcatatc aaccaggccg
      421 ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg tggaaatcgc ctgctcgccg
      481 ccttctttgg cactgcggaa ggtaaacagc agcggttttt ctggcatggt ttcacggaga
      541 atttttgccg ccgccatgac agactccaca ttggagaggt cggcaaagtg gtccacacgc
      601 cattccagaa tatcaaagtc cgcttcacga taggcgagag cttcggattt cacgcgggcg
      661 atatctttcg ccatcagcga gacgatgatt ttaggtgcgc ccgtaccaat gacgagatct
      721 tttacagtta cggttttcat tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg
      781 aaccccatga cctgtttaac atattccaga gggaaatctt tgccagtcca taacgtgaac
      841 tgttcagccc cttgccacaa caacatgccg tatccatcaa tcgttttgca accagcttgt
      901 tgcgcctgct gcaataactt cgtcatatgc gggttataca cgcattcagt gaccagcagt
      961 cccggatgta acagactgat atcattaact aatgattcat tctcaagggg tttcataccc
     1021 acttttgtgc cattggttaa aatgtcggcg gaagccaggg cttcagcaaa ggcttgctga
     1081 tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt cgttaacccg ctgcgcgaag
     1141 gcgagggctt tatcgaagaa ctcatcccga cggttaaaga gtttaatttc ttttaaacct
     1201 tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc cggcccctaa cagcaccatt
     1261 gttttgcctt tgatatcaaa accgctctct ttaatggcgc gaatatggcc cgtgccgtcg
     1321 gtgttatagc cgcgcaggta gccatcatca ttaacgatgg tgttgatggc acccaccagt
     1381 ttggctgctg gcgttaattc atcaacatat tcacacgcca gttgtttgtt cggcattgat
     1441 ataccagttc cgcgcatttt gagggctttt aatccttcaa ttgctccggg aaaactatcg
     1501 ttatccactt caaaggccat ataggtaaat ggcaatcccg ctttttctaa ggctttattc
     1561 tgcatttcag gcgataaact gtggcggata ggataggcca tcagcccaat caattcgtat
     1621 tttgcggtaa catccataat taactccttt aacctctatg cttaattgtg tttaaccggg
     1681 tgctaaattt acgttcgcca aaccgcacat cattctcagg aataataaaa acgcggtaat
     1741 agcggataaa aacaataatt gcggtaatca gggccagcag cgcgaaagta aaatcgagaa
     1801 caatgatata ttgaaggccg atgttcgaca gataaccggt aatcagcgga ataacaaagt
     1861 tagccagtcc acccatcatc atataaatac tggtgacttt ggctttgctt tttgggaaaa
     1921 actctgacat gaccgaaaca ccgagctgta aaatgccgcc agctgctgag aaaccgataa
     1981 caaaggcgcc ggcattacac accagtggag aagggtacag gtagataatg gctgctgtta
     2041 ttgttgccag tgcagagtta aatacattag cccagatggg ccggaccatt tttttcagta
     2101 gtgcggcaaa aataaagaca cagaccaacg agcccatact gtaataagag atggttttta
     2161 atgcctcagc ttctgacata ccagcaaaag ccatcgcata tttgggcatc cacaccacaa
     2221 tcacataaaa ggtcgagaat gcggctacac cgaacagtac cgatgaaaca ccttccagcc
     2281 agactaacgg tttgctgttc atttgcggta attcattagc tacgctggcg tccaccaact
     2341 ggctggggaa tttgcttttc aacagcatca gcgtgatcag tacaaacaga atacccggaa
     2401 taatcagccc atagccgtac cagatattat tgagcaacat atagctcacc agcattgggt
     2461 agaacatttg cccaaatgac accatcgctt taaccagtat gaccgccgaa ccagaggctt
     2521 tcggaaagca ttccatgagc gcggggtagc cacccgtatc cagcgctgag ttagcgatac
     2581 ctacgcacac tgccagaccg taggcgagag ttaaattcgg gcaagcggga ataccaaaga
     2641 agaatagcag atacattatt actgccatta atatcaccgc ccgacgacca aacttatcgg
     2701 agatcacacc gaagaataaa atactgatca atcgccccaa accaataccg gaaattaagt
     2761 aggcaatgcc cgcgttgtca gtggaaaact tttccgccag agatgacatg ttttgggcaa
     2821 gcgtaataac actaatgccg tgcaggaagt agctgaagta aatacaaaga acagccagga
     2881 taaatggcgt gctgaaagcc ttattttgag acattttcac agctccttgc tgaaagataa
     2941 tttcgttacc gtacgtattg ttcttcgacg gtttacataa ttactatact gaagatgatt
     3001 ttccctgggt acatggcata aagtttatgt ttccaggaat attgcatgtc atagtttgtt
     3061 tttgatcgcg attttcctta gttagcgcag aaagagttat ttatccgtaa gatcttgcat
     3121 gacctgaaaa gaatattgca ttacccaccc ggagcgacat tttcctttag cgagtgatgc
     3181 cgcgtttttt tgcggctgcg taagccgata aacagtgcca gtaaaaaacc gatggcagcg
     3241 atggcggtat cgaaccacat aatatcggca atacttcttt gggacagatg agccgtaatc
     3301 aacggaatag taaaggtcgc aatactgccc gcactgtaat agatccctgt agctttacct
     3361 ttagcgtaag ggaaacgttc agccattaac gtcaggccaa tttgcacaac acctccagca
     3421 gaggtaaaac caatgacaaa agcaaatatt atcaccacat aaaatgtggg atgcaggcag
     3481 acggtaaaca gagcaataaa tgagataaag gtgtacagca tcagtaatgt tgttgggcga
     3541 acggtattac gaatgagtgg agcggtaata aatacacaaa gcagcgaatc cacggtgtag
     3601 atactgagta gtttaatcga catagtgtat gacatgcctg caacaaattg tccgtactgt
     3661 gccagccact ggctaaccag ataaaacgtt gccattgaga tatagccata taaggtataa
     3721 ctggctaaat cgataattga acagcgatgt tccgtagagc tggtggtttt ctttatgaca
     3781 ggtaagcgat gacccggatg gggtgggaac gtacaacggt ataaaaacag agcgttaata
     3841 aacataatgc ctgcagcaat cataaaggac caaccgaacc acagttcagc ccacactaac
     3901 aggctaatga ttagcggtaa taaaaattgt ccgctggaaa caaatgcttt aattaaaata
     3961 ttggctgtcc caggtgagcg tggaaaagct tccatcaaac tgggataagt gcctgcatcg
     4021 agaaaactgt ttgccattcc tgccagaaag ccaaaaacat aagcgataat gatgttattg
     4081 gtgtgcagga tgccaaaaaa gaaggccata tagcagcaca tcccgagcat gataaaaggg
     4141 cggcgaccaa agcgatcgga taataatcct gcaaaaagca agacacttaa tcgaccaatg
     4201 cccagcgatg agataactat cgagacaccc gcggcattag tctgccaaag tgtctccagc
     4261 gaggccatat tcaggctcat caaaatgacg cccataccat gcactaggta attaaaatac
     4321 aacccaagtg cggtagggta atagggattt ttcataggtt ctacttagca aaggtgcttt
     4381 aaaaagtgta gcgatgttgt gtcagtatcc gttcattatt gttgtaacaa cgacaattcc
     4441 tttttgtgga gaaattaaca aagccgctcc agatcgtgtg catataattc agcggctacc
     4501 gattgataga tcttccagtc aataaaattg ccatcagggt agacttgttg aaacgcggtt
     4561 aagtcgggga aaaaacgcat ggtgttgttg ccgatgcggt tatttatttt ctctatgata
     4621 gcatgaagat gtttttttcg ttgctgacgc atagccaaca gttgctcgac tacacgatca
     4681 ggaatggcgc atttgccatt ttcccactga cgccagcttt cactgtcacc ggtctgggca
     4741 atccaggtcg cgcattcatc aatagtcatg gcaaaaatat ggcgaagtgc ctggagttca
     4801 taagcgttca tcgtgtaatg gctgcatgaa ataaacaata aaatataaat aacattatca
     4861 gcctgctgac ggcttgaaga gagtcacagt atcttgtgca acattattgg aaagagtgag
     4921 gggcgaggta gcgaagcgga aaaatgttaa aaaaaagccc atcgtgggag atgggcaaag
     4981 actacacaca gcaattcgtt gtttcactca ggggatttcc atgcttataa atcaatatgt
     5041 tgatttataa ccgtaagcta atagtaggca tacggacttt ttgcgtcgat cagattcgtc
     5101 tcattagttt aaaaactgta aagaatttgc gacaggtact accagctgaa tggcggtaag
     5161 caggaaaata tggggaagag caagcaaata tcaattagcg ggggttaaca atcaggcggc
     5221 agagccgcct gatgagaaat tacttattcg gtttctcgat ttcgccaagt tcttcgagaa
     5281 tttcttccgg ttggtccgtc ggcggcggga cttcttccac ccacgcggca aacagacgcc
     5341 aggaaacggc taacagaacc ggaccaataa acagaccgat catcccgaaa gcaatcaaac
     5401 caccaataac accagagagg atcaggatca gcggtaaatc ggcacccatg cgaattaaca
     5461 ttgggcggat gacgttatcc agcgtgccaa ccacaccgct ccacactaac aataccgttc
     5521 cccaggtggt atcgccagtc cagtagagcc agataatcgc cggaatcagt accggcaacg
     5581 ggccaagctg gacaaggcag gagaggatca ttaacaccgt tagcaacgtt gcataaggta
     5641 cgccggatac ggcgaggccg ataccgccaa gcactgcctg tactaacgcc gtcaccacca
     5701 cacccagcgc caccgcgcgg atagcctgtg ccgccagcag tacggcggca tctccgcgaa
     5761 cgcctgccag acgggttgca aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt
     5821 acagcagggc actgaagaga agcatcagcg cacaatgcac cataaagcgc ccgatatgcg
     5881 ccgcctgccc aacgaaccag gtggtggtgg tgccaatata agggcggact ttcgccatga
     5941 tcgccgtccc ccccatatcc agcaagttgt gccagcctgc atacagcttc gcgccaatca
     6001 ccggaatggt attaagccac gccagatcgg gtaacgtcat gtcgccgctg gaaatggctt
     6061 taattagcgg gccgctgccg tcgacgatac tgttaaccag caaagcaata gggatgataa
     6121 acaccatcac taataacagc gtcatcacca gaacggcgag agagcggcgg ccaaacatga
     6181 tcttttgcaa acgtaacaat accggccagg tggcgataac caccgtaccg gcccatgcaa
     6241 agccgagaat aaagggttga acaatccaca gacatgccac aatcatgatg gctaaaaaca
     6301 gcaccgaaag cagaatttgt gcgacatccc tgggctgacg aacatttacc ataactactt
     6361 ttcacctttt ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat
     6421 aatgatgagc aatttcagcg gtttttaaca ggccgattct gcccttaatt ctgatgggcg
     6481 tgttagaaaa aaatgtggta caacaactca ggcaacacgc aaacgattta ctcgtcgtat
     6541 ttcaagttgc atgtgctgcg gctacgttcg ttcaccccag tcacttacta ttgtaagctc
     6601 ctggagttca ctcgcttgcc gccttcctgc aactcgaaat ccatagagta aataaacatc
     6661 acctttcagt gcgctattgc agccgcctga aagaatagtg tatcgaccta catcacagac
     6721 cgcaggaaag ggtcaatata atgattccac agatttccca ggcacccggc gtcgttcaac
     6781 tggtgcttaa ttttttgcaa gagctggagc aacaaggttt taccggcgat acggcgacaa
     6841 gttatgccga tcgtctgaca atgtcgaccg acaacagtat ttaccaactt ctccccgatg
     6901 cggtggtatt tccgcgttca accgcagatg tggcgctgat cgcccgtctt gccgcgcagg
     6961 aacgctattc atcgctgatc tttacccccc gtggcggcgg caccggcact aacggtcagg
     7021 cgctcaacca ggggattatt gttgatatgt cccgccatat gaaccgcatc atcgaaatta
     7081 accctgaaga gggctgggtg cgcgttgagg ccggggtgat aaaagatcaa ctcaatcagt
     7141 acctgaaacc gttcggctac ttttttgcgc cggaactttc gaccagcaac cgggcaacgc
     7201 tcggtgggat gatcaatacc gatgcttctg gtcagggatc gctggtctat ggcaaaacgt
     7261 cggatcacgt acttggcgta cgtgcggtgt tgttgggggg cgatattctc gatacgcaac
     7321 ctttacccgt cgaactggcg gaaacgctgg gtaaatccaa taccactatc gggcgaattt
     7381 ataacacggt ttatcaacgt tgccgtcagc aacgccagtt aatcatcgac aacttcccca
     7441 aacttaaccg ctttcttacc ggttacgatc tgcgtcatgt ctttaacgat gagatgaccg
     7501 agttcgacct gacgcgcatt ctgacgggtt cagaagggac gctggccttt attaccgaag
     7561 cgcggctgga tattacgccc ttgcctaaag tgcgccgtct ggtgaacgtc aaatatgact
     7621 cttttgactc cgcgctgcgt aacgcgccgt ttatggttga ggcgcgggcg ctttcggtag
     7681 agacggtgga ctcaaaagtg ctgaatctgg cgcgggaaga tattgtctgg cattccgtca
     7741 gcgagttgat taccgatgtg cctgacaaag agatgctcgg gctgaacatt gtggagtttg
     7801 ctggtgatga tgaggcgctg attgacgagc gagttaatgc tctctgtgtg cggcttgatg
     7861 agctgattgc cagccaacag gcaggtgtga ttggctggca ggtgtgccgc gagctggcgg
     7921 gtgttgaacg tatctatgcg atgcgtaaaa aagccgttgg tctgcttggc aatgccaaag
     7981 gtgccgctaa gccaattccg tttgctgagg atacctgcgt accgccggaa catctggccg
     8041 attatattgc tgaatttcgc gcgctgctcg acagccacgg cttaagctac ggtatgttcg
     8101 gtcacgtcga cgcaggtgtc ttgcacgtcc gtccagcgct ggatatgtgc gatcctcaac
     8161 aagagatttt gatgaagcaa atctctgatg acgtagtggc gctgactgcg aaatacggtg
     8221 gtttgttgtg gggcgagcac ggcaaaggtt ttcgcgctga atacagcccg gcgtttttcg
     8281 gtgaggaact ttttgcagaa ctgcgcaaag tgaaagcggc atttgacccg cataaccgac
     8341 tcaacccagg gaagatttgc ccgccagaag gtctcgatgc gccgatgatg aaagtggacg
     8401 cggtgaagcg cggtacattc gatcggcaga tccccattgc ggtacgccag cagtggcgcg
     8461 gtgcgatgga gtgtaacggc aacggtttat gcttcaactt tgatgcccgt agtccgatgt
     8521 gtccgtcgat gaagatcacc cagaaccgga ttcattcacc gaaagggcgc gcaacgctgg
     8581 tgcgtgaatg gctgcgtttg ttggcggatc gcggcgttga tccactcaaa ctggaacaag
     8641 aactgcctga atccggcgtc agtttgcgga cgttaattgc ccgcacgcgc aatagctggc
     8701 atgcgaataa aggcgaatat gacttctcac acgaagtcaa agaggcgatg tcgggctgtc
     8761 tggcctgtaa agcgtgttcg acccagtgcc ccatcaaaat tgatgtgccg gagtttcgct
     8821 ctcgttttct gcagctctat cacacccgtt atttacgccc gctgcgcgac cacctcgtcg
     8881 ctacggtcga gagctacgcg ccgctgatgg cacgcgcgcc gaaaaccttt aacttcttca
     8941 ttaaccagcc gctggtgcgc aaactctcgg aaaaacatat cggcatggtt gatttgccgc
     9001 tgctgtcggt cccctcgcta caacaacaaa tggtggggca tcgctcggca aacatgacgc
     9061 tggaacagct tgaatctctc aatgcagagc agaaagcgcg cacagtgttg gtggtgcagg
     9121 acccctttac cagctattac gatgcgcaag tggtggcgga ttttgtccgt ctggtcgaaa
     9181 aattaggttt ccagcctgtg ttactgccat tttcgccaaa tggcaaagcc cagcatatta
     9241 aaggttttct taatcgtttt gcgaagacgg cgaaaaagac ggcggatttc ctcaaccgta
     9301 tggcgaagct gggtatgcca atggtgggcg tcgatccggc gctggtactt tgttatcgcg
     9361 atgaatataa actggcgctg ggcgaggaac gtggcgagtt taacgtctta ctggcgaatg
     9421 aatggctggc aagcgcactt gagtcacagc cagtggctac agtcagcggt gaatcatggt
     9481 atttctttgg tcactgtacc gaagttaccg ccttgccggg tgcgccagca caatgggccg
     9541 cgatatttgc ccgttttggc gcgaaactgg aaaatgtcag cgtgggttgc tgcggcatgg
     9601 cagggactta cggacatgaa gcgaaaaacc ataaaaattc gctcgggatc tatgagttat
     9661 cctggcatca ggctatgcag cgactgccgc gtaaccgctg tctggcgacc ggatattcct
     9721 gccgtagcca ggtaaaacgg gttgaaggca cgggggtacg ccatcctgtg caggctttac
     9781 tggagattat taaatgatat ggaaacggaa aatcaccctg gaagcactga atgctatggg
     9841 tgaaggaaac atggtggggt tcctggatat tcgctttgaa catattggtg atgacaccct
     9901 tgaagcgaca atgccagtag actcgcggac aaagcagcct ttcgggttgc tgcatggagg
     9961 agcatccgtg gtactggccg aaagtatcgg ttccgttgcc ggttatttat gtaccgaagg
    10021 tgagcaaaaa gtggttggtc tggaaatcaa tgctaaccac gtccgctcgg cacgagaagg
    10081 gcgggtgcgc ggggtatgca aaccgttgca tctcggttcg cgtcaccagg tctggcagat
    10141 tgaaatcttc gatgagaaag ggcgtttgtg ctgttcgtca cgattgacga ccgccatttt
    10201 gtgacgttgt caccagaaaa gtgtgacgca tattgccttt ggtcattatc tgcacagtaa
    10261 acggtggtat actgtgcagg tcttgtctaa aagcgaggat gctatgtcta ctcaacttga
    10321 tcccacccag ctggccattg aatttttacg ccgtgaccag agcaatcttt cacctgccca
    10381 atatctcaaa cgtctgaaac aactagaact tgaatttgct gacctgctta ctctctcttc
    10441 tgctgaatta aaagaagaaa tctactttgc ctggcgtctg ggcgtgcatt gatcctgact
    10501 taatgccgca agttcgccaa atcgacctgg attaacctcc gtccaggttc gatgaccttc
    10561 ttaatactgc ttatctaccg cctgatattg ttcaaaaata atactaatcg cagtttgtgt
    10621 taaaacggcg ggttagcttt atgagattat tcttatcgcc ccttcaagag ctaagccact
    10681 gagagtgccg gagataagcg ccggatgggg tagaaaccct taagcctgtg ttgcacagac
    10741 ttaagggttt tcttatttct gaacgctgca cttttttacc tatctcttaa atagtcattt
    10801 tcaatacaac tttttctttt tcatcacagc cttaatgctc cttagataat aaccattatc
    10861 taacaatgag atacctaatt cttagcgtgc ctgttaaccc acacatcagg gtctatgctt
    10921 attaaaagca ggcaaaaagc atgttttacg gtaaagcccc tgcgtttgct gggttgtact
    10981 gataatcatt atcactaaca tgctgttata cgctgaaagc gatgaagtga ggtaaatcga
    11041 tggacatgca ttcaggaacc tttaacccac aagatttcgc ctggcaaggc ttaacgctga
    11101 cacccgcagc ggcgatacac atccgtgagc tggtggcaaa gcagccgggt atggtcggcg
    11161 tgcgcttagg cgtgaagcaa acgggctgcg cgggctttgg ctatgtgctc gacagtgtta
    11221 gcgagccgga caaagacgat ctgctgtttg aacacgacgg cgcgaagctg tttgtcccgc
    11281 tgcaagcgat gccgtttatt gatggcacgg aagtcgattt cgttcgtgaa ggacttaatc
    11341 agatattcaa atttcacaac cctaaagccc agaatgaatg tggctgtggc gaaagctttg
    11401 gggtataggc ggtactatgt ctcgtaatac tgaagcaact gacgatgtca aaacctggac
    11461 cggcggcccg ctgaattata aagaaggatt cttcacccag ttagccaccg atgagctggc
    11521 aaaggggata aacgaagagg tggtgcgcgc aatttcggcg aagcgtaatg agccggagtg
    11581 gatgctgaag tttcgtctaa acgcctatcg cgcatggctg gagatggaag aaccgcactg
    11641 gttgaaagcg cactacgaca agctgaatta tcaggattac agctactact cagcaccatc
    11701 gtgcggtaat tgtgacgaca cttgcgcgtc tgaacctggc gcggtgcagc aaactggcgc
    11761 gaacgccttt ttaagtaaag aggtggaggc ggcgtttgag cagttgggcg ttcccgtgcg
    11821 ggaaggcaaa gaggtggcgg tggatgccat tttcgactca gtttcggttg ccactactta
    11881 tcgcgaaaaa ctggcggagc agggaattat tttctgttcc tttggtgagg cgatccacga
    11941 tcacccggaa ctggtgcgta aatatctcgg caccgtggtg ccggggaatg acaacttctt
    12001 tgccgcgctt aatgcggcgg tagcctctga cggtacgttt atttatgtgc ctaaaggcgt
    12061 gcgctgcccg atggaacttt ccacctattt tcgcattaac gcggaaaaaa ccgggcagtt
    12121 tgagcgcacc attctggtgg ccgacgaaga cagctacgtc agctacattg aaggctgttc
    12181 cgctccggtg cgtgacagct atcagttaca cgcggcagtg gtggaagtca tcatccataa
    12241 aaacgccgag gtgaaatatt ccacggtaca aaactggttc cctggcgata acaacaccgg
    12301 cggtattctc aacttcgtca ccaagcgtgc tttgtgcgaa ggcgaaaaca gcaaaatgtc
    12361 atggacgcaa tcagaaaccg ggtccgcgat tacgtggaaa tatcccagct gcattttgcg
    12421 cggcgataac tccattggtg agttttactc agtggcgctg accagcggtc atcagcaagc
    12481 ggataccggc accaagatga tccacatcgg taaaaacacc aaatcgacca ttatctcgaa
    12541 agggatctct gccggacata gtcagaacag ttatcgcggc ttagtgaaaa tcatgccgac
    12601 ggcaaccaat gcgcgcaatt tcactcagtg cgactcaatg ctgattggcg ctaattgtgg
    12661 ggcgcatacc ttcccgtatg tcgaatgtcg caataacagc gcacaactgg agcacgaggc
    12721 aacgacatca cgtattggtg aagatcaact gttttactgc ctgcaacgcg ggatcagcga
    12781 agaagacgcc atctcgatga ttgttaacgg tttctgcaaa gacgtgttct cagaactgcc
    12841 gctggaattt gccgttgaag cacaaaaact cctcgccatc agtcttgaac acagcgtcgg
    12901 ataaggaata aacatgttaa gtattaaaga tttacacgtc agcgtggaag ataaagctat
    12961 cctgcgtgga ttaagcctcg ccgttcgtcc cggcgaagtt cacgccatta tggggccaaa
    13021 cggttcgggc aaaagtacct tatcggcaac gcttgccggg cgcgaagatt atgaagtgac
    13081 gggcggcacg gttgagttca aaggcaaaga tttgcttgca ctgtcgccgg aagatcgtgc
    13141 aggcgaaggc atctttatgg ccttccagta tccggtggag attccaggcg tcagtaacca
    13201 gtttttcctg caaacggcac ttaatgcagt gcgcagctat cgcggccagg aaacgctcga
    13261 ccgctttgat tttcaggatt tgatggaaga gaaaatcgct ctcctgaaga tgccggaaga
    13321 tttattaacc cgttcggtaa acgttggttt ttccggcggc gagaaaaagc gcaacgatat
    13381 tttgcaaatg gcggtgctgg aaccggagtt atgcattctt gatgagtcgg actccgggct
    13441 ggatattgac gcattaaaag tggtcgccga tggcgtgaac tcgctgcgtg atggcaagcg
    13501 ctcattcatc attgttacgc actaccaacg cattctcgac tacatcaagc ctgattacgt
    13561 ccatgtgcta tatcggggac gaattgtgaa atccggcgat ttcacgttgg tcaaacaact
    13621 ggaggagcag ggttatggct ggcttaccga acagcagtaa cgcgctgcaa cagtggcatc
    13681 acttgtttga agctgaaggg acaaaacgct caccgcaagc acagcagcat ttacaacaat
    13741 tgctgcgtac cggactgccg acacgtaaac atgaaaactg gaaatatacg ccgctggaag
    13801 ggctgaccaa tagccagttt gtcagcattg cgggagagat atccccacag cagcgagatg
    13861 ccttagcgtt aacgttagac gctgtgcggc tggtatttgt cgatggacgt tacgtgtcag
    13921 cactgagcga tgcgactgaa ggcagcgggt atgaagtgag cattaacgac gaccgtcagg
    13981 gggtacccga cgctattcag gcggaagtgt ttctgcattt gacggaaagc ctggcacaaa
    14041 gcgtgacgca tatcgccgtg aagcgcggtc aacggccggc aaagccattg ctgttaatgc
    14101 atattaccca gggcgtggca ggtgaagagg tcaacactgc ccattaccga catcatctgg
    14161 atctggcgga aggtgccgaa gcaacggtga tcgaacattt tgtcagcctt aatgatgctc
    14221 gtcacttcac cggcgcacgg ttcactatca atgtcgcagc gaacgcccac ttgcagcata
    14281 tcaagctggc gtttgaaaac ccggtcagtc accactttgc ccataacgat ttgttgctgg
    14341 ctgacgatgc caccgcattt agccatagtt tcctgctggg tggcgcagtg ttacgacaca
    14401 acaccagtac gcaactcaat ggcgaaaaca gcacgctgcg gatcaatagc ctggcgatgc
    14461 cggtgaaaaa cgaggtgtgt gatacccgta cctggctgga acacaataaa ggtttttgta
    14521 acagccgaca gttgcacaaa actatcgtca gcgacaaagg ccgcgcggta tttaacggtt
    14581 tgatcaacgt cgcgcagcac gccatcaaaa cggatggtca gatgaccaac aataatctgc
    14641 tgatgggcaa actggcagaa gtggatacga aaccgcagct ggaaatctat gcagatgatg
    14701 tgaaatgcag ccacggcgcg acggtggggc gtattgatga tgaacagatg ttctatctgc
    14761 gctcgcgcgg gatcaatcag caggatgccc agcagatgat catttacgcc tttgctgccg
    14821 aactgacgga agcactgcgt gatgaggggc ttaaacagca ggtgctggcc cgaatcggtc
    14881 aacggctgcc aggaggtgca agatgacttt ttccgtcgac aaagtgcggg ccgactttcc
    14941 ggtgctttct cgtgaggtaa acggtttgcc gctggcttat ctcgacagcg ccgccagtgc
    15001 gcagaaaccg agccaggtga ttgacgccga ggccgagttt tatcgtcatg gctacgcggc
    15061 ggtgcatcgc ggtattcata ccttaagcgc ccaagcgacc gagaaaatgg agaacgtgcg
    15121 caagcgggca tcgctgttta ttaatgcccg ttcggcggaa gagctggtgt tcgtccgcgg
    15181 cacgacggaa gggatcaatc tggtcgccaa tagctggggt aacagcaatg tgcgggcggg
    15241 cgataacatc atcatcagtc agatggagca ccacgctaac attgttccct ggcagatgct
    15301 ttgcgcacgc gttggcgcag agctgcgtgt gatcccgctc aatcccgacg gtacgttgca
    15361 actggagacg ctgcctacgc tgtttgatga gaaaactcgc ctgctggcaa ttactcatgt
    15421 ctccaacgtg cttggcacag aaaatccact ggcggaaatg atcacgcttg cgcaccagca
    15481 tggcgcaaaa gtgctggtgg atggcgctca ggcggtgatg catcatccgg tggatgttca
    15541 ggcgctggat tgcgactttt acgtgttctc cgggcataaa ctgtatggcc ccaccggaat
    15601 tggcattctt tatgtgaaag aagccttgtt gcaggagatg ccgccgtggg aagggggcgg
    15661 ttctatgatc gccaccgtca gcctgagtga aggcacaacc tggaccaaag caccttggcg
    15721 gtttgaagcc ggtacaccca ataccggggg catcattggt cttggcgcgg cgctggagta
    15781 tgtttcggcg ctggggctta ataacatagc cgagtatgaa cagaatctga tgcactacgc
    15841 gctatcacag ctggaatctg taccggatct cactctctat ggtccgcaga acaggcttgg
    15901 cgttattgct tttaatcttg gtaaacacca cgcctatgat gttggcagtt ttctcgataa
    15961 ctacggcatt gctgtgcgta ccggacatca ctgcgctatg ccattaatgg cctattacaa
    16021 cgtccctgct atgtgtcggg cgtcgctggc catgtataac acccatgaag aagtggatcg
    16081 tctggtgacc ggactgcaac gtattcaccg tctgctggga taacagggag gcactatggc
    16141 tttattgccg gataaagaaa aattgctgcg taatttttta cgctgcgcca attgggaaga
    16201 gaaatatctc tacattattg agctgggcca gcgtctgcca gaattacgcg acgaagacag
    16261 aagcccacaa aatagcattc agggctgcca gagtcaggtg tggattgtca tgcgccagaa
    16321 tgcgcaggga attattgaat tgcagggcga cagcgatgcg gcgattgtga aagggcttat
    16381 tgcggtcgtc tttattctct acgatcagat gacgccgcag gatattgtca atttcgatgt
    16441 gcgtccgtgg tttgaaaaaa tggcgctcac ccaacatctc accccatctc gttcacaagg
    16501 tctggaagcg atgattcgcg caattcgcgc caaagccgct gcacttagct aaactaaagg
    16561 gacagctttc atccggttgg ctcacattgc cggatggtca atgtttacac tgacctgcac
    16621 aggagtgttt cggtttcagg tgaacataag cgccatccga cgtttcagcg tgagtctccg
    16681 gcaaatacag gaggtttaca atgaaacgcg cgtctttgct tacactcacg cttatcggcg
    16741 cttttagcgc catccaggct gcctgggcgg ttgattatcc gctaccacca accggaagcc
    16801 gactggttgg gcaaaatcaa acgtatacgg tgcaggaagg ggataaaaac cttcaggcca
    16861 tcgcccgacg ttttgatact gcggcaatgt tgatccttga agccaataac actatcgccc
    16921 cggtgccaaa acccggtacg acgataacta ttccttcgca actgttatta cctgatgcgc
    16981 cgcgtcaggg gattatcgtt aaccttgcag agctgcgcct ttattattat ccgccgggag
    17041 aaaatatcgt gcaggtttat ccgataggta ttggcttgca ggggctggaa acgccggtga
    17101 tggaaacgcg tgtggggcag aaaatcccta acccaacctg gacgcctact gcaggcattc
    17161 gtcaacgttc gctggagcgt ggtattaaat taccgccagt cgttcctgcc ggaccaaata
    17221 acccgctagg acgttacgca ctgcgcctcg cgcatggtaa tggcgaatac ctcattcatg
    17281 gcaccagtgc gccggacagc gtcggtttgc gcgtcagttc agggtgtatt cgcatgaatg
    17341 caccggatat taaagccttg ttctccagcg tgcggacggg aacgccggtg aaagtgatca
    17401 acgaacctgt gaaatattcc gtggagccta acgggatgcg ttatgttgaa gtacatcgac
    17461 cactatcggc agaagaacag cagaacgttc agacaatgcc atacacactg ccagcaggct
    17521 ttacgcaatt taaagacaat aaggctgtag atcagaagtt agtcgataaa gcgttgtatc
    17581 gtcgggcagg gtatccggtt tcggtgagca gtggagcaac tcccgcagcc agcaatgcgc
    17641 cttcagtaga gtcagcgcag aatggtgaac cagagcaagg gaatatgtta cgcgcgacgc
    17701 agtagcggta aacggcagac aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca
    17761 ggtactatta cttgcggtat ttagtagcca tgttgtccag acgctggtta gcacgagctg
    17821 cgtcatcttt agcagcctga acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt
    17881 caactttagc gttcagagtc tgaacgtcag aagacagctg atcgatttta gcgttgctgg
    17941 agcaacctgc cagcagagta gaacccagga ttaccgcgcc cagtaccagt ttagtagctt
    18001 tcattattaa taccctctag attgagttaa tctccatgta gcgttacaag tattacacaa
    18061 agttttttat gttgagaata tttttttgat gggaaggcac ttatttttga tcgttcgctc
    18121 aaagaagcat cgaacgctgg gttttagata aaaaaattga gagaaaaca
//
LOCUS       DAZFRJ010000083        18117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000083 DAZFRJ010000000
VERSION     DAZFRJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.083"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(78..884)
                     /gene="trpA"
                     /locus_tag="ACHKUN_002592"
     CDS             complement(78..884)
                     /gene="trpA"
                     /locus_tag="ACHKUN_002592"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO6660828.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(884..2077)
                     /gene="trpB"
                     /locus_tag="ACHKUN_002593"
     CDS             complement(884..2077)
                     /gene="trpB"
                     /locus_tag="ACHKUN_002593"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO6660829.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2089..3450)
                     /gene="trpCF"
                     /locus_tag="ACHKUN_002594"
     CDS             complement(2089..3450)
                     /gene="trpCF"
                     /locus_tag="ACHKUN_002594"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO6660830.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3451..5046)
                     /gene="trpD"
                     /locus_tag="ACHKUN_002595"
     CDS             complement(3451..5046)
                     /gene="trpD"
                     /locus_tag="ACHKUN_002595"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO6660831.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5046..6608)
                     /gene="trpE"
                     /locus_tag="ACHKUN_002596"
     CDS             complement(5046..6608)
                     /gene="trpE"
                     /locus_tag="ACHKUN_002596"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO6660832.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6630..6723)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6700..6744)
                     /gene="trpL"
                     /locus_tag="ACHKUN_002597"
     CDS             complement(6700..6744)
                     /gene="trpL"
                     /locus_tag="ACHKUN_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO6660833.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6882..7763
                     /gene="rnm"
                     /locus_tag="ACHKUN_002598"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6882..7763
                     /gene="rnm"
                     /locus_tag="ACHKUN_002598"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO6660834.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7760..8380
                     /gene="yciO"
                     /locus_tag="ACHKUN_002599"
     CDS             7760..8380
                     /gene="yciO"
                     /locus_tag="ACHKUN_002599"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO6660835.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            8481..9353
                     /gene="rluB"
                     /locus_tag="ACHKUN_002600"
     CDS             8481..9353
                     /gene="rluB"
                     /locus_tag="ACHKUN_002600"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO6660836.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9393..9983)
                     /gene="cobO"
                     /locus_tag="ACHKUN_002601"
     CDS             complement(9393..9983)
                     /gene="cobO"
                     /locus_tag="ACHKUN_002601"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO6660837.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(9980..10738)
                     /gene="yciK"
                     /locus_tag="ACHKUN_002602"
     CDS             complement(9980..10738)
                     /gene="yciK"
                     /locus_tag="ACHKUN_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO6660838.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10958..12007
                     /gene="sohB"
                     /locus_tag="ACHKUN_002603"
     CDS             10958..12007
                     /gene="sohB"
                     /locus_tag="ACHKUN_002603"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO6660839.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12043..12294)
                     /gene="yciN"
                     /locus_tag="ACHKUN_002604"
     CDS             complement(12043..12294)
                     /gene="yciN"
                     /locus_tag="ACHKUN_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO6660840.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12674..15271
                     /gene="topA"
                     /locus_tag="ACHKUN_002605"
     CDS             12674..15271
                     /gene="topA"
                     /locus_tag="ACHKUN_002605"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO6660841.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15481..16455
                     /gene="cysB"
                     /locus_tag="ACHKUN_002606"
     CDS             15481..16455
                     /gene="cysB"
                     /locus_tag="ACHKUN_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO6660842.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            16774..16914
                     /gene="ymiA"
                     /locus_tag="ACHKUN_002607"
     CDS             16774..16914
                     /gene="ymiA"
                     /locus_tag="ACHKUN_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO6660843.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            16917..17084
                     /gene="yciX"
                     /locus_tag="ACHKUN_002608"
     CDS             16917..17084
                     /gene="yciX"
                     /locus_tag="ACHKUN_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO6660844.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            17198..17293
                     /gene="ymiC"
                     /locus_tag="ACHKUN_002609"
     CDS             17198..17293
                     /gene="ymiC"
                     /locus_tag="ACHKUN_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO6660845.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            17457..>18117
                     /locus_tag="ACHKUN_002610"
     CDS             17457..>18117
                     /locus_tag="ACHKUN_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO6660846.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
BASE COUNT         4443 a         4555 c         4759 g         4360 t
ORIGIN      
        1 tattaaatga atttaggatt tttccggctt cattaaagaa agttaaaatg ccgccagcgg
       61 aactggcggc tgtgggatta actgcgcgtc gccgctttca tcggttgtac aaaaactttc
      121 agtgccgcca gcattttctc tggctcatta atatgttgct cgatgatttt aacaatggcc
      181 gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg cttttacctg atccggggcg
      241 gaaataccaa atccctgcaa tggaggtgca gcgttgtact ctttcagctt cgcaaccaga
      301 tgattgaggg gtaacgcggc gcggttttct gcgccggtca cgcctgctcg tgacagcaaa
      361 taggtgtaac cacgaccgta agaggctatc tggcgcagca ggtcgtcatc ggcatttggc
      421 gggcagatga agataggtgc gacattatga cgcaacgcgg cctggcggaa gggcgcggac
      481 tcttcaactg gcacatcggc aaccagcacc gaatcgacgc cgactttttc gcactgggca
      541 taaaactcat caatgccttt gttaaacacc agattggcat acatcaacag gccaatggga
      601 atggtcgggt gtttctggcg aatcagtgcc agcatttcaa aacattgtgc cggagtcaca
      661 cctgccgcaa aggcgcgcag agtggcgttt tgaatcgtcg ggccatccgc cagtgggtcg
      721 gagaagggga tacctaactc cagcgcgtca gcaccggctt caattagcgt atcgataatt
      781 ttcaatgact gctcaatgcc cggatcaccg agcgtgacga aaggaacgaa tgcgccttct
      841 ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt ccatcagatt tcccctcgtg
      901 ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc gcgaccggaa aggttaacca
      961 ccagtagctg ctctttatcc gggttttcgc gcatcatttt caacgcatgg gccagggcgt
     1021 gggaggattc cagcgccggg atgatccctt cgtgcaggca cagcgttttg aaggcttcaa
     1081 gggcttcatc atcggtaata gacacgtaat cagcgcgtcc agtgctgtta agatacgcgt
     1141 gttgtgggcc gacagacggg aaatccagtc cggcggagat ggagtaagat tcttcaatct
     1201 gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa atagataccc acgcgaccat
     1261 gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg accacctggc tccacaccaa
     1321 tcaggccgac gttggtttca ttgatgaaat cagcaaacat gccgatggca ttcgaaccgc
     1381 cgccaacaca ggcgataacg gcatccggca ggcgaccttc tctttccaga atctgcgctt
     1441 tggtttcttc gccaatcatc cgctgaaact cacgcacaat ggtcggataa ggatgcgggc
     1501 cagctgcggt gcccagcata tagtgcgcgg tttcgtaact accggaccag tcgcgcagcg
     1561 cctcgttaca ggcatctttc agcgtcgcgg aaccgctatg caccgggatc acttccgcac
     1621 ccattaagcg catacgaaaa acgttaggcg actggcgttc aacgtctttg gcacccatat
     1681 aaatacggca tttcaggccg agcagggcgc tggcaagggc cgacgccacg ccatgctgac
     1741 cggcaccggt ttcggcgatg atttcggttt tacccatccg cttcgccagc aacgcctgcc
     1801 ccagcacctg gttagtttta tgcgcgccgc cgtgcagcaa atcttcacgc ttgagataca
     1861 gcgtagtgtt cgtcccggct gtaatgttct ggcatttggt cagcgcggtt ggacgcccgg
     1921 catagttttt cagcaggtcg ttgaactgag cctgaaattc aggatctttt tgcgcactga
     1981 caaaagcttc ttccagctgg cgcagagcag gcatcaggat ttgtggcacg tacatgccgc
     2041 caaactcacc aaaatagggg ttaagtaatg ttgtcattgt tcctttcctt aatatgcgcg
     2101 cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg atgcccggtt gcgactctac
     2161 agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc gcttccacgc agttatctgc
     2221 gcctaagccc cccgccagca gaacgttgcc aagcgattga ccatttaata gtgaccagtc
     2281 aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca tatttatcaa cgtgctgaaa
     2341 ctcgcgggcg ggcagggttt caccgacgct taatgctttc cagatggcaa catgtgctgg
     2401 cagagcttca cgcagcgtat cgatatacag ctgttcttca ttaccatgca gttgcactgc
     2461 cgccagcgat aacaccttag ctttgtccac cacatcggca atatcgtgat tgcggaacac
     2521 gccaacatac tgcaacggtg ccgcagccat cacttcctgc gcctgttcaa cgttgacgca
     2581 acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc gcgcccgcgt cataagctgc
     2641 tttagcatct tgcccacgcg tcaggccaca tactttattc tcacccagca acacccggcg
     2701 cacggcggcg tgcaaatcgt catgggccat caacgccgaa ccaatcagaa aaccgttagc
     2761 gaagtggctt aactcgcgca cctgagcgta agtattgatg ccggattcgc tgattaccgt
     2821 cacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg ttgagatcaa tcgacaaatc
     2881 acgcagatcg cggttgttga tgccaacgac ctttgccccc aatgcaatgg cacgctccag
     2941 ttcctcttca ttactgactt cggtcagcac acccatctcc agactgtggg cgacggcggc
     3001 aagctggcga tattgttcgt catccagtac tgaaagcatt aataagcagg catcagcctg
     3061 gtaatagcgc gccagataga tctggtaagg atcgataatg aagtctttac ataaaatcgg
     3121 ctgcggggcg atttggctga cgatggggag gaaatcaaag ctcccctgaa aatatttctc
     3181 atcagtcagc accgaaattg ccgaagcgta atgtttataa atggcggcaa tgcgtgctgg
     3241 atcgaaatca tcacggatca cgccttttga cggcgacgat ttcttgcact ccagaataaa
     3301 cgccgtgcgc gcaccctgta gcgcatcata aaaatgtcgc gtgctcggct gaacctcatt
     3361 ctgaaaactg gccagcggtt gctgctgttt gcgggtttct acccaaatcg ccttgtctgc
     3421 gacgattttc gctaaaacgg tttgcatcat ttaccctcgt gccgccagtg cggtaactct
     3481 gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt tgcgcattgg cttgcagatc
     3541 ttcatggcca tgcaggcgca ttaacatggc gacgttcgca gcgacggctg cttcatgggc
     3601 ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca cggttttctt ccggtgttcc
     3661 gcctgccagt tgctcctggt ggtagggagt caggccaaaa tcttcagcgg tcaattgata
     3721 gctcttaatt tcgccgtcat gcagctcggc aacgattgtc ggcgcgtgta atgaaacttc
     3781 atccatcccg ccgctgtgca ccaccgccgc gcgttgatac cccagcacgc gcaaggtttc
     3841 ggcaatcggc agcaccagtt ccggactata aacaccaatt aacgccagcg gcggatgcgc
     3901 cgggttaatc aatggcccca gcacattgaa cagggtgcgg gttttcagtt gctggcgaac
     3961 cggcatcgcg tggcggaatc cggtgtgata cttcggcgca aagaggaaac atacacctaa
     4021 ctcatccagc gcctggcgcg atttatcggc gttcatatca agattaatac cgaacgccgc
     4081 cagcagatcg gacgaaccag atttactgga gacgctacgg ttgccgtgtt tcgccacttt
     4141 cagcccacag gccgcggcga caaacgcact ggcggtagaa atattgatac tgttgctgcc
     4201 gtcaccgcca gtaccgacga tatcagcaaa cagataatcc gggcgcggga acggcgcggc
     4261 gttttccagt agcgcggttg ctgctccggc gatctcgttc gggtgctcac cgcgaatttt
     4321 catgctcacc agcgccgccg ccagttgttc cggcttcagc tcgccacgca ccaccgctga
     4381 aaacagctgg tggctttctt gttggctaag cgtctgcgcc tgatacagtt tttccagaat
     4441 cggttgcagc gtgttggctg gctctagttt ctgctgcgcc caggccagcg tttgttccag
     4501 caggcgagcg ccctgggtgg tgagaatgga ttccggatgg aactggaatc cacaaacgcg
     4561 atccgcatcg tgacgtactg ccatcaccat gccattaaaa tgggcgttga tggttaaacc
     4621 ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc accggcagcg ggtttgttaa
     4681 tccggcaaac atcgcctgac cgtcatgttc aatgctcgac gctttaccgt gaagaatttc
     4741 gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt gcctgatgtc cgaggcaaat
     4801 gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt tccggcatac aaccggcttc
     4861 gctcggcaca ccggggccag gagaaagcat cagtaccgga ttgctcatcg tcgccaggcg
     4921 ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc accacattat gcccattgct
     4981 gcgcaactga tctgccaggt tgtacgtaaa agagtcgata ttatcgagca gcagaatgtc
     5041 agccatcaga aagtctcctg tgcatgatgc gcggtggcaa tagcgcgcag tacagcgcgg
     5101 gctttattac gggtttcgtc ggcttccgac tgcggaacag aatcaaggac tacgccagca
     5161 ccggcttgca cggtggcgat accgttttcc accagcgccg agcggatcac aatgcaggtg
     5221 tcgagatcgc catgcgcggt gaaataacct accgcgccgc cgtagctgcc gcggcgacga
     5281 ccttccgcct cggcaattaa ctgcatagcg cgtactttcg gcgcaccact taacgttccc
     5341 atattcatac aggcgcggta agcgtgcagg gcgtcgagat cgtggcgcag ctcaccgacc
     5401 acgcgggaga ccaagtgcat cacgtaagag taacggtcaa ctttggtgag gtcggcgacg
     5461 tagcggctgc cgggggtgca aatgcgtgcc agatcattac gggcgagatc caccagcatc
     5521 agatgttcag aaagctcttt atgatcggta cgcatttcca gttcgatgcg gctgtcgagg
     5581 tctctgtcca gtgaaccatc ggcacgacga ccgcgcggtc gtgttccggc aatcgggtag
     5641 atctcaatct ggcggctggt ggcgtcatac ttgagcgaac tttccggcga cgcgccaaac
     5701 agggtgaaat cattatcctg cataaaaaac atgtacgggc tgggattact ctttttcagc
     5761 acgtaatagg ccgccagcgg tgacgggcag ggcagagaga aacggcgaga tggcaccacc
     5821 tggaaaattt ctccggcgcg aatcgctttt tgcaacaaac gcactacgcc accgaactct
     5881 tcatcgctct ggttacattc acaacgcata tgcggcacgg aaaccaccgg cagcggtggc
     5941 gcggtttcgg tcagttgctg gcgaagatcg ttcaggcgag cagtgagacg ttgtttttct
     6001 tcttcattcg gagcaaacag gctggcctga atgcgggtgc tttttttctg atggtcaatc
     6061 accatcagcg tttcagcgag ataaaaacag aaatcaggac agttattttc cgctgacaat
     6121 tgcggtaaat cttcaaaccc cgccacaagg tcataagaga acaggccgcc gaagaacatt
     6181 gcttctcgtt cttccttcgg tacattcagc atattctgca ataagcggaa agcgtcaaaa
     6241 accgaaaggg agcataagcg agcgtcttca tccagcagtg gactgacagg ggggaagcgc
     6301 agcacacggc agtttggtaa ttgttcattt tccatacccg caggcagggc gttatccagt
     6361 agtgtcagca gggcttcgcc gttgccggaa agtgcctgga ttgtgacagt gtcacctaaa
     6421 gctgtaatgc gcagcgcact gtctaccagc agcaggcttt ttaaatcatc tttgctgtcg
     6481 atatctgcgg attccagcag cagcgttgcc ggacgatccc cacacaactg gtgaaaaagc
     6541 gcggtgggat tgtcgcgata agcgccttcg caggttagca gttcgagagt cggtttttgt
     6601 gtttgcattg ttattctcta attttgttca aaaaaaagcc cgctcattag gcgggctggg
     6661 tatctgattg ctttacgcat ggtgaataca ctgcccgttt caggaagtgc gccaccaacc
     6721 tttcagtacg aaaattgctt tcattgtcga tacccttttt acgtgaactt gcgtactagt
     6781 taactagttc gatgattaat tgtcaatagc tcatttcaga atatttgcca gaaccgttat
     6841 gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg
     6901 cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag
     6961 cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca
     7021 ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg gcgttgaatc
     7081 ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat attgtcgggc
     7141 tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag acagaacggc
     7201 gcaatcagcg ggcgcagctg attgccgaaa gacttgaaaa agcgcaaata cctggggcgc
     7261 tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat tttgcgcgtt
     7321 tccttgttga gtgcggcaaa gccagttcaa tggcggatgt ctttaaaaag tatctggcgc
     7381 gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct attgatgtca
     7441 ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat cttagtgcta
     7501 aatggctgaa aagattggtt gcgcattttg ccgaacacca cggtgacgcg atggaagtcg
     7561 cgcagtgcca gcaatcgccc aatgaacgta cccagctggc ggcccttgcg cgtcagcatc
     7621 atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc gaactgggtc
     7681 gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa cagccgcaga
     7741 acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata acccacagca
     7801 acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg tttatccaac
     7861 tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgcgatgg agcgtatttg
     7921 tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga
     7981 actctcgacc tattcatttg ttgataacgt tgcgtttcgt ttaatgaaaa acaacacgcc
     8041 gggcaactat accttcatct tgaaggggac gaaggaagtg ccacgccgtc tgttgcagga
     8101 aaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg cgttgcttga
     8161 agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg aatttaccga
     8221 atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga ttattcacgg
     8281 cggctatctc gggcagaaac cgacaacggt tattgatctt accgatgata agccggtcgt
     8341 ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact ctgtatacta
     8401 cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg cctgtgaagg
     8461 cgacactaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc cgcgctggcc
     8521 acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt gttgatggca
     8581 aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt cgtatcgatg
     8641 gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg gcctattaca
     8701 aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg gtgtttgacc
     8761 gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac gttaacacct
     8821 gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg catccaagcc
     8881 gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc
     8941 gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa accatcaagt
     9001 tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa ggccgtaacc
     9061 gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg atccgtgttc
     9121 gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg gagctggacc
     9181 tcgcccagac taactatctg cgcgaactgg tggagctacc gccagaaacc agctctaaag
     9241 tcgctgtaga aaaagaccgt cgtcgcatga aggcgaatca gattcgtcgt gcggtgaaac
     9301 gtcacagtca ggtgagcggc ggtcgtcgtt ctggcggacg taataacggt taatcaaagt
     9361 atgccggacg tcatatccgg catttttaca gattagtaat caatccctat ctgcgctttt
     9421 acaccggcat caaacgcatg tttgacgggg cgtaattcac ttaccgtatc tgccagttca
     9481 agaatatccc gatgacaacc acgacccgtg ataatcaccg tctgttgatg tggacgttca
     9541 tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat atacgtcagt
     9601 tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc atgttgccag
     9661 acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt aaagcccgtt
     9721 gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc attaggccag
     9781 gtgcctttaa taaactgcac gacacctact ttttttccgt gaccaactgc gcgtgttgcc
     9841 gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat gataataccg
     9901 cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg ctgctgacgc
     9961 tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt tgggcgtcaa
    10021 aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag agcggcatga
    10081 tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctg gcacgcattg
    10141 cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga tattcatcgg
    10201 ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca ccccagttag
    10261 ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg tccgatttga
    10321 gtaataaagg aagcagcgcc tgggtgagca aaaaggtggc attaacgttg acctgcatga
    10381 cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg agcaatccgg
    10441 cattatgcaa aacaccatcc agacgcggat aattaacggt aatgcgctgt gccagttgtt
    10501 ggcaatcttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc tgacgtccag
    10561 tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg cccaacagaa
    10621 tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acggccaatc ccatcgctgg
    10681 ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg taatgcatgg
    10741 ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt tggggcttta
    10801 tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa aatggatact
    10861 ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc tgttgtaaac
    10921 tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg
    10981 tttgtttttg gcgaaaatcg ttaccgttgt gctagcgatt gcggcgattg ccgccattat
    11041 tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca acaatctcag
    11101 cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggact cacatcagca
    11161 aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc
    11221 gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg tgctggattt
    11281 taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa cggcggtact
    11341 cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg gtggcatggt
    11401 gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa acattccttt
    11461 aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct gtgtggcgga
    11521 caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg cgcaaatgcc
    11581 caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca ctgccgggca
    11641 gtataagcgt actctgacct tgctgggtga aaataccgaa gaagggcggg agaaattccg
    11701 cgaagagttg aacgaaacgc atcagttgtt taaagatttt gtgaagcgta tgcgtccgtc
    11761 tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg cggtagagaa
    11821 aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga tggaaggccg
    11881 tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat tcaccggcag
    11941 cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc aaaagccatt
    12001 gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa ccagatgata
    12061 tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg tgcttttggc
    12121 tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca cgccattacg
    12181 ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac gaatgatttt
    12241 gttggcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt tcattattta
    12301 ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat ataccacgct
    12361 ggtggcaaga gcgccttact ggtaactttg gattttgcat gctaataaag ttgcgtatcg
    12421 gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg cttgacattc
    12481 gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga cctgttaact cagtcacctg
    12541 aattttcgtg aacagagtca cgacaagggg ttgatatccg cagagaacga gtccatatcg
    12601 gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt
    12661 aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa aagccaaaac
    12721 gatcaacaag tatctgggta gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga
    12781 tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca ccaagacggc
    12841 taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg
    12901 gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg tctctgaact
    12961 gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg accgcgaagg
    13021 ggaagccatt gcatggcacc tgcgggaagt gattgggggt gatgatgcgc gctatagccg
    13081 agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaata aaccgggtga
    13141 gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg
    13201 gtatatggtt tcgccgctgc tatggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt
    13261 gcagtcggtg gcggttcgcc tggtggtcga gcgtgagcgt gaaattaaag cgttcgtgcc
    13321 ggaagagttc tgggaagtcg atgccagcac gaccacgcca tctggtgaag cgttggcgtt
    13381 acaggtgact catcagaacg acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc
    13441 tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac
    13501 aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg ccagcacccg
    13561 tcttggattt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg aagcaggcta
    13621 tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa atatggttcg
    13681 cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga atcagtacgc
    13741 cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg tcaatgtgat
    13801 ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagt taatctggcg
    13861 tcagttcgta gcctgccaga tgaccccagc gaaatatgac tccacgacgc tgaccgttgg
    13921 tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgttttgatg gctggacaaa
    13981 agtgatgcct gcgttgcgta aaggcgatga agatcgcatc ttaccagcag ttaataaagg
    14041 cgatgctctg acgctcgttg aacttacacc agcccagcac tttaccaagc cgccagcccg
    14101 tttcagtgaa gcatcgctgg ttaaagagct ggaaaaacgt ggtatcggtc gtccgtctac
    14161 ttatgcgtcg atcatttcga ccattcagga tcgtggctac gtgcgagtag aaaatcgtcg
    14221 tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa atttccgcga
    14281 gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg tggcaaatca
    14341 cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc agcagttaga
    14401 taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg ttctgaccag
    14461 cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagca ccggggtatt
    14521 ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca ccattaacct
    14581 ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa ccaacgcgct
    14641 gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc tcatcgatcc
    14701 gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg agatcgaaga
    14761 gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa aatgtggctc
    14821 tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca acgaagagtg
    14881 taaaaacaca cgtaagattt tacgtaacgg cgaagtggca ccaccgaaag aagatccggt
    14941 gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc gtgacggtgc
    15001 tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc gtgcgccact
    15061 ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt atctggccga
    15121 tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc gtaaaaccaa
    15181 acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag cattttatgt
    15241 tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctgtcaggtt tttataaaca
    15301 aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca cctatacact
    15361 aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa atcgtattag
    15421 tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg atggtttaac
    15481 atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct gaatgtctca
    15541 tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt cagaatgctg
    15601 gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac gcaggtaacg
    15661 ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt cgatgccata
    15721 aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat cgccaccacg
    15781 catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga gcgttatccc
    15841 cgcgtttctt tgcatatgca ccagggctcg ccgacacaaa ttgctgatgc cgtctctaaa
    15901 ggcaatgctg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga tttagtgatg
    15961 ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc gctggcaggc
    16021 aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata taccttcggc
    16081 tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac gccgcgtatc
    16141 gtcttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg gctgggggta
    16201 ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt gcgtgttgat
    16261 gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag tactttcttg
    16321 cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg tgatgtcgtt
    16381 gatgcggctg tcgcattgcg ctctaatgaa gaaattgagg tcatgtttaa agatataaaa
    16441 ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg aaggtgccgg
    16501 aatatattct cttttgttac ttatttctat accgtgcaca atatttttat ccctcatatt
    16561 tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaac tccagttttc
    16621 aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg taaattcgct
    16681 ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt acaccaatcg
    16741 ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc ttctggaaat
    16801 caggaacccc gccgcgatcc tgaattaaaa cgtaaagcct ggctggcggt ttttcttggt
    16861 tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg ataactatgg
    16921 tcggtcagga gcaactggag tcgtcaccat tatgccagca tagtgacaat gaaacagaaa
    16981 cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg cagcagcaag
    17041 cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca tcattcttat
    17101 tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca gcttgagtta
    17161 tctcaacaca aaataataac cgttaagggt gtagcctatg atcaacacaa atatgaaata
    17221 ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac tcctctggat
    17281 cattactcac tgatccttga ccccgctgcg gcggggttgt catttgcttt gccacaaggt
    17341 ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt gtgttatctt
    17401 taatattcac cctgaagaga atcagggctt cgcaaccctg tcattaagga ggagctatgt
    17461 cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa acttaccact
    17521 actacagcct gccgcttgct gctaaatcac tgggcgatat cacccgtcta cccaagtcac
    17581 tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg taactcggtt accgaagagg
    17641 atatccacgc gctggcagga tggctgaaaa atgcccatgc tgaccgtgaa attgcctacc
    17701 gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt gatctggcgg
    17761 caatgcgcga agcggttaaa cgcctcggcg gcgatactgc aaaggttaac ccgctctcac
    17821 cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat gatgaggcat
    17881 ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg ttcctgaaat
    17941 ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc aggcacaggc atttgccatc
    18001 aggttaacct cgaatatctc ggcaaagcag tgtggagtga attgcaggac ggtgaatgga
    18061 ttgcttatcc ggatacactc gttggtactg actcgcacac cactatgatc aacggcc
//
LOCUS       DAZFRJ010000084        18044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000084 DAZFRJ010000000
VERSION     DAZFRJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.084"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(264..1265)
                     /locus_tag="ACHKUN_002611"
     CDS             complement(264..1265)
                     /locus_tag="ACHKUN_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6660847.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1372..1668
                     /locus_tag="ACHKUN_002612"
     CDS             1372..1668
                     /locus_tag="ACHKUN_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6660848.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1802..2227)
                     /gene="arsC"
                     /locus_tag="ACHKUN_002613"
     CDS             complement(1802..2227)
                     /gene="arsC"
                     /locus_tag="ACHKUN_002613"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO6660849.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2240..3529)
                     /gene="arsB"
                     /locus_tag="ACHKUN_002614"
     CDS             complement(2240..3529)
                     /gene="arsB"
                     /locus_tag="ACHKUN_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO6660850.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3583..3936)
                     /gene="arsR"
                     /locus_tag="ACHKUN_002615"
     CDS             complement(3583..3936)
                     /gene="arsR"
                     /locus_tag="ACHKUN_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO6660851.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4676..4759
                     /gene="dinQ"
                     /locus_tag="ACHKUN_002616"
     CDS             4676..4759
                     /gene="dinQ"
                     /locus_tag="ACHKUN_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO6660852.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4813..6165)
                     /gene="gorA"
                     /locus_tag="ACHKUN_002617"
     CDS             complement(4813..6165)
                     /gene="gorA"
                     /locus_tag="ACHKUN_002617"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO6660853.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6237..7079)
                     /gene="rlmJ"
                     /locus_tag="ACHKUN_002618"
     CDS             complement(6237..7079)
                     /gene="rlmJ"
                     /locus_tag="ACHKUN_002618"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO6660854.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7282..9324
                     /gene="prlC"
                     /locus_tag="ACHKUN_002619"
     CDS             7282..9324
                     /gene="prlC"
                     /locus_tag="ACHKUN_002619"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO6660855.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9332..10084
                     /gene="rsmJ"
                     /locus_tag="ACHKUN_002620"
     CDS             9332..10084
                     /gene="rsmJ"
                     /locus_tag="ACHKUN_002620"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO6660856.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10133..11602)
                     /gene="dtpB"
                     /locus_tag="ACHKUN_002621"
     CDS             complement(10133..11602)
                     /gene="dtpB"
                     /locus_tag="ACHKUN_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO6660857.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11768..11821
                     /locus_tag="ACHKUN_002622"
     CDS             11768..11821
                     /locus_tag="ACHKUN_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660858.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11920..12354)
                     /gene="uspA"
                     /locus_tag="ACHKUN_002623"
     CDS             complement(11920..12354)
                     /gene="uspA"
                     /locus_tag="ACHKUN_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO6660859.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12745..13080
                     /gene="uspB"
                     /locus_tag="ACHKUN_002624"
     CDS             12745..13080
                     /gene="uspB"
                     /locus_tag="ACHKUN_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO6660860.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13325..14824)
                     /gene="pitA"
                     /locus_tag="ACHKUN_002625"
     CDS             complement(13325..14824)
                     /gene="pitA"
                     /locus_tag="ACHKUN_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO6660861.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15056..16258
                     /gene="yhiN"
                     /locus_tag="ACHKUN_002626"
     CDS             15056..16258
                     /gene="yhiN"
                     /locus_tag="ACHKUN_002626"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO6660862.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16574..17626)
                     /gene="yhiM"
                     /locus_tag="ACHKUN_002627"
     CDS             complement(16574..17626)
                     /gene="yhiM"
                     /locus_tag="ACHKUN_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO6660863.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4714 a         4511 c         4599 g         4220 t
ORIGIN      
        1 aaatgtgttg tattagcgac tgaaatctgc cttttaaaaa ggaggagggt actgagacaa
       61 agagtcattg cttctccgat aaaatgtcag agaagcatga ttacgtggtt attgtctgtt
      121 gatgacttgt tgaccgagcc gtcatcattc tcaatgcatt agttaaatca tttgtgattt
      181 ttaaggatta tcctgtctat gattttctac ttagaaggat atggcactaa ctatgcatct
      241 tatagcttac tgctggcatc tatttaaaat agcaattgag tcaaatagcc catcaagata
      301 gccataccta atataaccgt caggaatgca gctatcatcg gcattctgaa cattgattta
      361 agtaatatca cttccgtcag gcttgcgccg gcactgccga tgattaatgc cattaatgct
      421 cccagaccca ttccttttgt catcagaaca gatgccagag ggataactgc ctcagcccgg
      481 atatacagcg gaataccgac aacggcgctt aatgggatgg cgaagggatt atctgctcct
      541 gcatgagcgg caatccacgc tgagggaata aagccataaa taaaagatcc tattaatact
      601 cctaacaaaa gatatggcag tacatcttta aactgttgca acgcatcttt ggtggctagt
      661 tttatgggat taatacttac tacagctttg gtactgcaac aggtaactac ggggttctca
      721 atggctttag ccgctggact acaacaactc acttttatgg gcgtatatgt cgttcccggc
      781 gaagtttttg ctggagcaca acaatttgct gatgagcttt tactggcaat gatatgacgt
      841 tcaaacccca gagaatccag gataatactg gcaagaacgg agacgccggc tgcgataatc
      901 gcgtacaaca aggtgacttt ccaaccaaag gtcatccaca ttagtccgac gataatggga
      961 ttaagtaatg gtgaaacaaa taaaaaagtg agagtcggac caaacccggc tttcgctgac
     1021 aacaatccac gtaacatggg tattgtcgag caactacaga acggggtaac ggctcccaac
     1081 agagcagcca ggagatagcc tctccctttt ctggccccca tcatctgctg gattttatgg
     1141 tctggcacct tttgtcttat caggctgaca ccggcactaa tcactataaa cagcaacgaa
     1201 agctcgacgg cgagaaacac aaacatctct gcggcatctt gcagcatcgc aagccaacta
     1261 ctcatacgta acctcattgg ataaatctat atttccagaa taatcgaaat gaaattcaga
     1321 tcaagatatt tctggtatta tcgaaatatc aaccagacag aggggtgtca tatgcagttg
     1381 gaagaagtcg ctaaagcgct gaaagagcta ggtcacccga cccgtttgtt catatttaaa
     1441 catcttgtca aagccgggga acaagggttg ccggtcggtg agttgcaaaa gcagcttggg
     1501 atacctggct ccacactcag ccaccatata tcagcactgg tatcggtagg actggtcaaa
     1561 caaaaccgtg agagtcggac gctgatgtgt gtttcgcaat acgcaatgct ggaagcgata
     1621 attgaattct tacgcgagga gtgctgcgta aatagcaaaa ttgcctgatg cgctacgctc
     1681 tacatatttc atgccatata ttgaatttgc acggttttgt aggtcggata agacgcgtta
     1741 gcgtcgcatc cgacataaac aaagcgcact ttaccaacta tcggcagtgg atatcgccgc
     1801 tttatttcag gcgcttaccc gcttcatcaa ccactttctc gccatcttcc ttggagaatg
     1861 cgcctttttg cgcatctggc agaatttcca gcaccacttc tgaagggcgg cacaggcgag
     1921 ttcccagcgg cgtcaccaca atcgggcgat taatcagaat cgggtgctga agcataaagt
     1981 cgattaaccg atcgtcagta aatttatctt ccgcaaggcc cagctcctca tacggttcga
     2041 cgtttttacg cagcagcgcg cgtacggaaa tccccatatc ggcaatgagt ttgaccagtt
     2101 catcgcgcgt tggcggagtt tccagataat ggataatggt cggttctgtg ccgctgttgc
     2161 ggatcatctc cagcgtatta cgcgacgtgc cgcaggccgg gttgtgataa atggtaatgt
     2221 tgctcatatc agtatctcat tacaaagtga aagagagacg tagcgccagc gcagccagcg
     2281 tcacaaacag cacaggcagg gtcatgataa tccctgtacg gaagtaatat ccccagctga
     2341 tagtcatatt cttctgcgaa agtacgtgca gccagagtag cgtagccagg ctaccaattg
     2401 gggtaatttt cggtcccaaa tcgcagccaa tcacattggc ataaaccatc gcttctttga
     2461 taacgccaga tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat
     2521 tgttcataat agaagagagg aaggcggtga ggaatccggt gccgagcgtc gcggcccaca
     2581 ggccgttatc cgccagcacg ttgagtacgc cagaaagata ttccgttaat ccggcattgc
     2641 gcaggccata aaccaccaga tacatgccga gcgagaagat gacaatctgc cagggggcac
     2701 cgcgcaggac tttacccgta ttaatcgcat gaccgcgttt agcgacgaca aataatatca
     2761 gcgcgcccac agctgcaatg gcgctcaccg gaatgccgag cggttccagg acgaaaaatc
     2821 ccaccagcag aagcagtaaa acaacccagc cagttttgaa cgtagcagga tctttgatcg
     2881 cttctgcggg agatttcagc agcgccatat cgtagttctg cggaatatct ttgcgaaaat
     2941 agagatgtaa catcaccagc gtggcaacaa tcgcggcgat atccaccggc accatcaccg
     3001 aggcgtattc gcgaaagccg aggccaaaga aatcagcgga aacgatattc accaggttgg
     3061 agacaataag cggcaggctg gcggtatcgg caatgaatcc ggccgccatc acgaacgcca
     3121 gcgtagtgcc tttactgaac cctaaagcca gcagcatggc gatgacaatc ggtgtcaaaa
     3181 taagcgccgc gccatcattg gcaaacaggg cggcaacggc agcaccgagc aggacaatcc
     3241 aggtaaacag caagcgacca cgaccattac cccagcgtga gacgtgcagc gccgcccatt
     3301 caaaaaagcc ggactcatcc agcagcaggc tgatgataat gacggcgata aacgcagccg
     3361 tcgcgttcca gacgatattc cacaccaccg gaatatcacc cggatggacc acgcccgtaa
     3421 ctaacgccag tactgcgccg agcgttgcac tccagccgat gcctaaacct ttcggctgcc
     3481 agataaccaa tacgatggtc aggacaaaga tagcgcctgc cagtaacata atgcctcccg
     3541 gataaaacac atctgaaaat tcatatgtgt ttagctaaat ttttaactgc aaatgttctt
     3601 actgtccccg gaacagtttt gtcgagccag gttgcggaca atcgcctgaa ccttttcctg
     3661 ttcacatcgc caggcctcat caataatttt cgccgcccat gctggaatat gcggtgataa
     3721 gcggtaatga acccacttac cttgcttgcg gtccagcaat agcccgcttt cacgcagcaa
     3781 tgccaggtgg cgggagatct tgggctgcga ctggtcgaga gcagtgcaga gatcgcagac
     3841 gcataactct cccagttcgc tgagcagtaa aacgatgccc agacgggttt catcagcaag
     3901 aattttgaac aattggatgg gtaacagaaa tgacatattg cgctcctgat tgttgcaggt
     3961 agtgtctctc ttcgaagcgg ataagtcaaa aacatatatg acttaacgaa tgtgtaagtg
     4021 cagaggaagg taataggtgt gaattttgag ttggctattc atttgaaagg aggtctgaat
     4081 caggacgtaa aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc
     4141 tctggctgga gtgagagagt tcttatctaa cagctcaata ctattagcca gtggctaaca
     4201 ttgcaagaat tagcgatttc ttcagctggc ggagggggca cttcggtata tatcgcaatg
     4261 acgatttata tactttcact gggtcatcgt catattaagc cttgtccgaa tccggatgta
     4321 aaaaaaccga ctttgcgtcg gttttttact ttccagccct gagttggtgg cactggctgg
     4381 agtgagaatg ccattatcta acgcatcaat gctaatacca tactgaaact attgcaagga
     4441 catgctggtt ttataacctg aatgtactgt atgattatcc agttagctct gaggcatttt
     4501 cactctggta atgcgcataa acgctttcaa agtcctggtc agaagtacgg gtggtgccgt
     4561 taactgatgc tctggccgga gtgagaatgc cattatctaa caatagggca tgcgccgtga
     4621 caggcagtgg atgagtaagc ggatgcattc tcactccatc gcatggagaa aacgggtgat
     4681 tgataaagca atcatcgttc ttggggcgtt aattgcgctg ctggaactga tccgctttct
     4741 gcttcagctt ctgaactgat agcggaaacg taattaaggg ctaagagcac actactctta
     4801 gccctttaac atttaacgca ttgtcacgaa ctcttctgcc gccgttgggt gaatggcgac
     4861 ggtattgtcg aagtcttttt tggttgcacc catcttcagc gccaccgcga agccctgcaa
     4921 catttcgtcc ataccaaagc caatgccgtg aataccgaca atcttctctt ccgatccaac
     4981 gcacaccagc ttcatgcggc acggctggcg gtgagtggtg acggcggtat acatcgcggt
     5041 gaaagaggat ttatacactt tcacctgatc gtcgccatac tgctcgcgcg cctgcggttc
     5101 cgttaaacca acagtaccaa tcggcggatg gctgaagacc acggtcggaa tgttgctgta
     5161 atccagatgc tcatccggct tgttattaaa caggcgttca gagagacggc gacccgctgc
     5221 aactgccacc ggtgtcagct ccactgcacc cgtgttatcg cccaccgcgt aaataccttc
     5281 aatattggtg ttttgatatt tatcgacgac gatatagcct ttttcgttag ttttaacgcc
     5341 agcggcttcc aggttgatgt tgtcattggc aggctcgcga ccaatcgccc aaatcaggca
     5401 atccaccgtt tcactgcgac catcttccag ctccagcgtc aggctaccat cggcattttt
     5461 cactaccgct ttcgggatgg cgttggtgtg cagctgcggg ccttcggcgt tcatcacttc
     5521 gaccagcgtt tcggaaatca tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa
     5581 cagatgcgtt ttcgcgccga ggccgttaat cacgcccgcc agctcaacgg cgatgtaacc
     5641 cgcgccaaca accgccacgc gctctggcaa agcaggaagg gcgaagaagc catcagaatc
     5701 aataccgtat tccacgcccg gaatatccgg gtggctcgga cgaccgcctg tggcgatcag
     5761 aatatgatcg gccgtgatgg tttcgccgtt tacctccagc gttttggcat caacgaagcg
     5821 ggcaaagcct ttgattacat caacgttatt tttaccgagc acgttttcat aggaagtatg
     5881 aatacggtcg atataggcgg tacggctggc gatcaacgtt tcccagttga atttattgat
     5941 agtggtatca aaaccataat ccgggccgta catatggatc gcttcacgga tttgcgccgc
     6001 gtgccacatc acttttttcg gcacacagcc aacatttacg caggtgccgc ccagctcttt
     6061 ggcttcaatc agcgcacatt tctggccgta catagccgcg cggttgatgg aggcgatacc
     6121 gccgctgccg ccgccgatgg cgatgtaatc atagtgttta gtcatgacaa agtgtcctta
     6181 tcgttgatta ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg ctgcaattac
     6241 tccggcacga tccagcttac ggtggcgtgc ccggtgcctg ccggaaccag tttgctgtgc
     6301 agccacggca gcacgttatt catctgctgt tccagtttcc acggcgggtt aatcacaatc
     6361 atgccggaag cggtcatgcc acggcgatcg ctgtctggca gtaccgccag ttcaatttgc
     6421 agaattttgc gaataccggt cgcttccaga tcgtggatca tgcgcttaat ttgctgacgc
     6481 agcaccaccg gataccacag tgcgtaagta ccagtggcga aacgtttgta accttctgct
     6541 atcccgctga ccaccgcctg atagtcggtt ttcatttcat acggcgggtc gataaggatt
     6601 aaaccacggc gggaaaccgg cggcagtttg gccttaagct gctggaaacc atcggctttt
     6661 tcgacgcgcg cacggctgtc tttctgaaat tcagaacgca gcaacgggta atcgctcggg
     6721 tgcagttcgg tcagttgcag gctgtcttgt tcgcgcagta gctggcgagc aatcaatggc
     6781 gaaccggggt agtaacgcaa ctgaccgcta cggttgaagt gttttaccac attgatgtac
     6841 gcctccagtt ctgcgggcaa atcgtcctgc tgccagatac gggcgatgcc ttcgagatat
     6901 tcgccggtac gctcggcatg ttcgctgcct aactgataac gcccggcccc tgcgtgggtg
     6961 tcgagataga gaaacggttt atctttctct ttcagcgact cgatgatcag gctctgaacg
     7021 gtatgtttaa ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata actgagcatg
     7081 ggtaaaggtg ttccggtaag taaaaatcgg cccgtattcg gggcgcacaa aagcgtatca
     7141 ggacagtata ccgaaaagag gccgccgccg cgaaagcgta acgtttctca ttgaaattca
     7201 ctacacttaa ccccatgcta cacacattat gtaaagcgcc tgttgagcgc ttccttaacc
     7261 tctttaacca ggactgcgcg aatgacgaat ccgttactga ctccctttga attgcctccg
     7321 ttttctaaaa ttctcccgga acatgtcgtt ccagccgtga ctaaggcgct gaacgactgc
     7381 cgcgaaaatg tggagcgcgt agtagcgcaa ggagcaccgt acacctggga aaatctctgc
     7441 cagccgttgg cggaagtgga cgatgtgctg gggcgtatct tctccccggt cagccacctg
     7501 aactcggtga aaaatagccc ggaactgcgt gaagcgtacg aacaaaccct gccgctgctg
     7561 tcggaataca gcacctgggt agggcaacat gaagggctgt ataaagcgta tcgcgacctg
     7621 cgcgatggcg atcattacgc cacgctgaac acggctcaga aaaaagcggt tgataacgca
     7681 ctgcgcgact tcgaactctc tggcatcggt ctgccaatag agaaacaaca acgctacggt
     7741 gagattgcca ctcgtctttc cgagctgggc aaccagtaca gcaacaacgt cctcgatgcg
     7801 acgatgggct ggaccaaact cgttaccgac gaagcggagc tggcggggat gcctgaaagc
     7861 gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg
     7921 ctggatatcc caagctacct gccggtaatg acctactgcg acaaccaggc cttgcgtgaa
     7981 gagatgtatc gcgcttacag cacccgcgcc tccgatcaag gcccgaacgc cggtaaatgg
     8041 gataacagca aggtgatgga agagatcctc gctctgcgtc acgaactggc gcaactgctg
     8101 ggctttgaaa actacgcctt taaatccctt gccactaaaa tggcagaaaa cccgcagcag
     8161 gtgctggatt tcttaaccga tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg
     8221 gcacaactgc gcgccttcgc caaagcggaa tttggcgtcg atgagttgca gccgtgggat
     8281 atcgcgtact acagcgaaaa acaaaaacag cacctctaca gcatcagcga cgagcagctg
     8341 cgtccgtact tcccagaaaa caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt
     8401 tacggcatca ccgctaaaga gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc
     8461 ttcgaactgt atgacgaaaa taacgaactg cgcggcagct tctacctcga tctgtatgcc
     8521 cgtgaaaaca agcgcggcgg ggcgtggatg gatgactgcg taggccagat gcgtaaagct
     8581 gatggttcgc tgcaaaaacc ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat
     8641 ggtaaaccgg cgctgttcac tcacgaggaa gtgatcaccc tgttccacga gttcggtcac
     8701 ggcctgcacc atatgctgac ccgcatcgaa accgctggtg tttccggtat cagcggtgtg
     8761 ccgtgggatg cggtcgaact gccgagtcag tttatggaaa actggtgctg ggagccggag
     8821 gcgctggcgt ttatctccgg tcactatgaa accggcgaac cgctgccgaa agagttgctg
     8881 gataaaatgc tggcggcgaa gaactaccag gcggcgctgt ttattctgcg tcagctggag
     8941 ttcggcctgt ttgatttccg ccttcatgcc gagttccgcc cggatcaggg ggcaaaaatc
     9001 ctcgaaactc tggcagaaat caagaaactg gttgccgtgg tgccatctcc gtcctggggc
     9061 cgtttcccgc acgctttcag ccatattttc gccggtggtt atgcggcagg ttactacagc
     9121 tacctgtggg ctgacgtact ggcggcagat gctttctcgc gctttgagga agagggcatt
     9181 ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc tgagccgtgg cggttcagaa
     9241 gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg
     9301 gagcattacg gcattaaggg ctgatcattc agtgaaaatc tgcttaattg atgaaacagg
     9361 cgccggagac ggcgccttat ctgttctggc ggcccgctgg gggctggagc acgatgaaga
     9421 caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc gtgatgagcc
     9481 aaaacttggc ggcatatttg ttgattttgt tggcggagcg atggcgcacc gacgcaaatt
     9541 cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg attatttgcc
     9601 ggatgtggtg gacgccaccg cagggctggg gcgcgatgcc tttgtgctgg cttcagtcgg
     9661 ttgccgcgtg cggatgctgg agcgtaatcc agtcgttgcc gcattgctcg acgacggcct
     9721 ggcgcgtggt tatgcggatg cggaaatcgg cggctggttg caggagcgtt tgcagttaat
     9781 tcacgcctcc agcctgacgg cgctaaccga tattacccca cgcccgcagg tggtctatct
     9841 cgacccgatg ttcccgcata agcagaaaag cgcgctggtg aaaaaagaga tgcgtgtctt
     9901 tcagtcgctg gtggggccgg atcttgatgc cgatggattg ctggagcctg cacgcttgct
     9961 ggcgaccaaa cgcgtggtag taaagcgccc ggactacgcg ccaccgctag cgaatgtcgc
    10021 cacgccaaac gcggttgtga ctaaagggca tcgctttgat atctatgcag gtacgcccgt
    10081 gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat aattaatggc
    10141 tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc actactgcaa
    10201 cgcccagcgt gaccagacca atcttaccaa acacgttggt atagacgggc aacgtctcaa
    10261 gcggatcggt aatgttgtcc ggcaccgcgg taaatgttgc cacatagccg cccagcaaga
    10321 acgcggcagc ctgcgtcagg aaccacatcc cgagaataaa gcccatcaaa tgctgcggca
    10381 ccagggcagc aatcatcgcc aggccaaggg cgctaataaa cagttcacct aagctctgga
    10441 ataagtacac cagcacgata aaccatggcg atgtcagccc ttgtgcatcc gcaaaccaca
    10501 ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat aaacatgccg agagtaaatt
    10561 tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg cctgccagta
    10621 ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc gggttgatgg
    10681 aaaaaccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc gatgttggca
    10741 tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag gcgacaaaca
    10801 ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg atggtgacga
    10861 cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc agccatgcac
    10921 atacgaagat catcaccacg ctgccaagta atacgtacaa cagtttgctg aagctcatcg
    10981 gcttgaagtc gggttcagaa ccaatgtctt tcaccattcc acgacaggcg atgtaaacca
    11041 gtaatgcgat aattaacccc gccccgcaca ggttgtaggt gactgaataa ccgaatctat
    11101 cagcgatcac aggggccagc gataacgcta tcaacgagcc gatattgatc gacatataga
    11161 acagggtgaa tgcgccatca agccgcggat ctttcggcgg atagcacttc gaaagcaagc
    11221 tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg gcgatgaaaa
    11281 tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc agcacaagtg
    11341 ctccgagaac aatggtgcgt ttggtcccca gcaggtggtc gccgacatag ccgccaatgg
    11401 aaatgaggcc atagaccagc gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg
    11461 agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga
    11521 atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc
    11581 ccatgggtgt tgttgtattc atatttatta accttccaat accatcctga aaagtatgaa
    11641 cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa tattgatgcg
    11701 gtgaatagat taatacacta ttgcaacaga aaataaccag ctgatcacac taatgatgga
    11761 aaatatgatg gtgttaaatg ttgtattggc aggagatttt gattgcgtac gcagatcgta
    11821 ggccagataa ggggtttacg ctgcatcagg taacaaggcg cgccaaaaag caaaacgccc
    11881 gcatatgcgg gcgttcagga acacgtcgtg gagggatgat tattcttctt cgtcgcgcag
    11941 cggaacaatc agcatatcaa cgtgaacggt gttgatcagc tgacgtgcgg aagacatcag
    12001 tttgctccag aagtcctggt ggtgaccaca aaccaccaga tccatatcgt atttcttgat
    12061 tgcatcgacc agaacctggc ccaggtcgcc gctgccgctc agggtttcag tgattgggta
    12121 gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc tcttcagaga tgcgtttctg
    12181 catatcaccc agattcacat caataagccc ggtgtatagg tcagagtagt ttacatctac
    12241 gtggatcaga gaaactttcg cattgtaggg gcgagccata gagactgctt tctctaccag
    12301 aactttgctt tccggggaga gatcgactgc gatgagaatg tgtttataag ccatagtgtt
    12361 actccttcca taaagttgtc gatgactggc cagctagcgt ttcttgtgcc gttacgtcac
    12421 ccgcgtcctg cgaactacgt gcgcggggct aacccagccg taaagattca gtgttaagac
    12481 catccttacc ttatagcgac ccggatgatc cgtcaatccg ccttgcttac ccgttagata
    12541 aacaaatttt cgacatgaat atattgatag tggttaacct tctggaaaaa aaacaacctg
    12601 atctcctaca ctatctatag agccgctcgg atgttggtca ccacaaaagc ggtttctcca
    12661 ggacctggtg ttaactgatt ggcagcgtat cgggagcggt agccccgggg tggaactccg
    12721 cgggcaggtc gccggggagg aggtatgata agcaccgtcg cattattttg ggctttatgt
    12781 gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac tacgcgcgtt gttagtggta
    12841 ctgcgtaact gcgatccatt gctctatcaa tatgttgatg gagggggctt ttttacctca
    12901 catggccaac ccaacaaaca ggtgcgtctc gtttggtata tctatgccca acgttatcgc
    12961 gatcatcacg atgatgaatt tattcgccgc tgtgagcggg tgcgtcggca gtttattctg
    13021 accagcgcat tgtgtggtct ggtggtggtc agcctgattg cattgatgat ttggcattaa
    13081 gcacttcagc aataataacg cataaaaaaa gcgggccagt aaactgaccc gctcgagact
    13141 gattacaaac gcaaaattgc ctgatgcgct acgcttatca ggatctacat aatctctgca
    13201 atctattgaa tttgcatgtt tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
    13261 aacaaagcgc actatgtcac aatctgaagc gggccagctg acccgctcgt tttggtgtgt
    13321 acgattacag gaactgcaag gagagccagt acagtccgcc ggaaagcagt accgcagccg
    13381 gaagggtaaa cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc
    13441 catctaccac catcgtcccc gcgacagaag aggagagtac gtgagtagtg gaaaccggca
    13501 tcccggtata actcgccagg ccgatagaca ctgccgccgt catctgggcc gacatcccct
    13561 gagcgtaggt catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacggcgcc
    13621 agccaatcat cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggtg
    13681 catactcgat ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag
    13741 cactcacacc aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc
    13801 ggcgcatctg gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtagtca
    13861 acatgccttt caggcggttg agcgcgttaa tggtattcga cggatggcaa tggaactccg
    13921 cgggttgcgt tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt
    13981 tgagcagcgc aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac
    14041 gggtgatttc gtagccagtg gcattcatgt tcaccacgaa gcctgctggt gcgacgccaa
    14101 tcaataccaa cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg
    14161 ccacgccgat agcggaaagg atcagcgcaa tacgcgtcca gaacggcggc tttttcttgc
    14221 cgtctttctt ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc
    14281 tccagtagcg acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg
    14341 aaacgatcag agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg
    14401 acgtcccggt catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat
    14461 gagagctgga tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca
    14521 gcaacataga gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg
    14581 tcggcagcat atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa
    14641 agttgaacac cgctgccata accacggcga gctgagaacg catcgcgcgg gtatagataa
    14701 cggttgccac ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa
    14761 aagccagtgc aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta
    14821 gcataggacg ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg
    14881 gcgcgggcaa agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa
    14941 aaaataatta tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg
    15001 cgaccactga ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga
    15061 aaggtttgat gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc
    15121 aggtcaggca ggacgccggg ttctgctgat cgataatggt aaaaaaccag ggcgcaaaat
    15181 ccttatgtct ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta
    15241 tctgagccag aatccgcatt tttgtaagtc tgcactcgcg cgttttaccc agtgggattt
    15301 cattgatctg gtcaataaac acggcatcgc ctggcacgag aaaacgttag gacaactctt
    15361 ctgcgatgac tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa
    15421 tgtaaccttc agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac
    15481 gcttgaactg aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct
    15541 gtcaatgccg gggctgggcg cgtcgccgtt tggttataag attgccgaac aatttggcct
    15601 caacgtgctg ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga
    15661 agagttacag gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcat
    15721 cgttttccgt gagaacttac tcttcaccca tcgcggcttg tctggaccgg cggtgttgca
    15781 gatttccagc tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga
    15841 tctcgaaacc ttcctgaatg agcagcgtaa cgcacatccg aatcagagcc tgaaaaacac
    15901 actggcggtt catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc
    15961 ggatgtttcg ctaaaacagc tcaacgtgcg tgaccaacag gcactgatta gcacattgac
    16021 cgactggcgc gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg
    16081 cggcgtggac accaacgaac tctcttcacg gacgatggaa gcgcgcaaag tgcctgggct
    16141 gtacttcatc ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg
    16201 ggcgtggtcg agtgcgtggg cttgtgcgca ggatttgatt gcagcgaagt cgtcctgacc
    16261 tgttacctaa agatgaaaat tattagagcc gcattaaaaa tgagctaatt ttgatagtgg
    16321 ctatcttgtg attattttct aatgagcccg tgaactgaaa ccctccaggc ttaatataag
    16381 gtggaaggaa aggtgattga aaactcaccc agtggccaga tctttataaa atatgaacag
    16441 gttgaaaaaa acagtaactt tgttgttttt ttatccttaa ccactatgca ttaatgctgc
    16501 gttatttcat gatgcctaag aaaaaccagc gttacgcaaa tggtcaacgc tggttttatc
    16561 cggtacgttg caattatttt ttagcagaac ctgcttctaa tattgaaacg attgagaaca
    16621 acgtaaagca taccgctcct aaaccgacca aaacccgcga aggaataaaa tatcccatgt
    16681 cggtctgcgc catttccgca agaaacgatg ccaggaaaag gcaaaacagg caggtgaaga
    16741 cgggaatcat cggtattctg ttggctaacg aacaggtacg tctccatacc agtgccagta
    16801 gccagacttt tgagaatatg ctgtaacaga tcattccgag gcaaataaga ataatgccgg
    16861 gagccagtcg ggcgcttgca tcggaactga ctaagacgta tatcccctgc agtaccgtga
    16921 ttgagccgag aaaaataacc caataacacc acagccagtg ttctttagtt gaaaacgtat
    16981 tacgtgtttg atggacaatt gtggcaacaa ggccaattag acaggcacag attgcggtta
    17041 acccaagcaa tacgtgaccc gcgacataat gcggagttat gtcagcacta cgtaacagcg
    17101 taatagacca aatgaaaccg agcagggtga gtaaaacggg aacggcaatg aggcaattac
    17161 cgattaatga agaataagcc tgtaccggcg ttccatcgct cttgctcccc gctgcatttt
    17221 tgggaatgag cagaaagtga ccggatgacg ctgccaccgt cgatacacag gcggcaatca
    17281 taccaacgcc gaaaataaca tggccggcga caaactcgtc tgccatcaca tcattgcctg
    17341 ctaacagtgc ccaaccccat atcatggtga taattgaccc cgcatagcca ataatgggga
    17401 acaatgtatt gtaaaacgta ttaacgccgc gcgtgagctg cgaaatgata ataaatgcgg
    17461 tagtgaataa tgccaggcat atggctgcca gagaaatcag cacatgaccc gcaacgaaat
    17521 actcactttg cccagaactt tccagcacaa agccacctaa cgcgatacaa attaagccca
    17581 taattaaagg gattaatttg aaaagccacc cgatatatat gttcacttta aactcctcag
    17641 gtatccctgc taatattgaa atgtgcatta atgataattt cagcctgtta atataatgac
    17701 gagatatcat taaagacaaa tgcatagcat aactatagcg ggatttactt tgcagagcgc
    17761 tacttttcag cgttcaaaag gccatggtaa ataaatattt atcctaagat taattcttat
    17821 tctgttgttt tttacaatta tcttccaggg agggattctt tttcattatt tggtaaaaga
    17881 ccggatggta tcggtgctta aaatcagcga acatataagt attattgttt tatatcgtcg
    17941 aaaatatgtc tttcagacat gtattattta ttttgttact aacaagtcaa aaatatcgca
    18001 ggttatttat gaaagcaatt gacaatatat ttaagtacgt ccct
//
LOCUS       DAZFRJ010000085        17543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000085 DAZFRJ010000000
VERSION     DAZFRJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.085"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(156..1019)
                     /locus_tag="ACHKUN_002628"
     CDS             complement(156..1019)
                     /locus_tag="ACHKUN_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO6660864.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(991..2541)
                     /locus_tag="ACHKUN_002629"
     CDS             complement(991..2541)
                     /locus_tag="ACHKUN_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO6660865.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2799..3578
                     /gene="deoC"
                     /locus_tag="ACHKUN_002630"
     CDS             2799..3578
                     /gene="deoC"
                     /locus_tag="ACHKUN_002630"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO6660866.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3705..5027
                     /gene="deoA"
                     /locus_tag="ACHKUN_002631"
     CDS             3705..5027
                     /gene="deoA"
                     /locus_tag="ACHKUN_002631"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO6660867.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5079..6302
                     /gene="deoB"
                     /locus_tag="ACHKUN_002632"
     CDS             5079..6302
                     /gene="deoB"
                     /locus_tag="ACHKUN_002632"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6660868.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6382..7101
                     /gene="deoD"
                     /locus_tag="ACHKUN_002633"
     CDS             6382..7101
                     /gene="deoD"
                     /locus_tag="ACHKUN_002633"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO6660869.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(7262..7525)
                     /locus_tag="ACHKUN_002634"
     CDS             complement(7262..7525)
                     /locus_tag="ACHKUN_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6660870.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(7557..8573)
                     /gene="lplA"
                     /locus_tag="ACHKUN_002635"
     CDS             complement(7557..8573)
                     /gene="lplA"
                     /locus_tag="ACHKUN_002635"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO6660871.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(8601..9245)
                     /locus_tag="ACHKUN_002636"
     CDS             complement(8601..9245)
                     /locus_tag="ACHKUN_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO6660872.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            9351..10319
                     /gene="serB"
                     /locus_tag="ACHKUN_002637"
     CDS             9351..10319
                     /gene="serB"
                     /locus_tag="ACHKUN_002637"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO6660873.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            10368..11750
                     /gene="radA"
                     /locus_tag="ACHKUN_002638"
     CDS             10368..11750
                     /gene="radA"
                     /locus_tag="ACHKUN_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO6660874.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            11771..13003
                     /gene="nadR"
                     /locus_tag="ACHKUN_002639"
     CDS             11771..13003
                     /gene="nadR"
                     /locus_tag="ACHKUN_002639"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO6660875.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13310..14977)
                     /gene="ettA"
                     /locus_tag="ACHKUN_002640"
     CDS             complement(13310..14977)
                     /gene="ettA"
                     /locus_tag="ACHKUN_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO6660876.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            15188..17125
                     /gene="sltY"
                     /locus_tag="ACHKUN_002641"
     CDS             15188..17125
                     /gene="sltY"
                     /locus_tag="ACHKUN_002641"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO6660877.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            17215..17541
                     /gene="trpR"
                     /locus_tag="ACHKUN_002642"
     CDS             17215..17541
                     /gene="trpR"
                     /locus_tag="ACHKUN_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO6660878.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         4017 a         4574 c         4892 g         4060 t
ORIGIN      
        1 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
       61 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
      121 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
      181 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
      241 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc gttcaggcgt
      301 accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg ttgcaaataa
      361 tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg ctctgccagc
      421 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg atgacattcg
      481 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
      541 ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg cagttgcgga
      601 tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt ggcttcgccg
      661 ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct aagcacctca
      721 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta
      781 tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc
      841 tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat cgtccacgga
      901 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
      961 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     1021 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     1081 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     1141 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat
     1201 tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt
     1261 ttcgtcgagg gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac
     1321 agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg cgcccggcgt
     1381 ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt tttgccagcc
     1441 atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac
     1501 ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct cacacagcaa
     1561 gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat
     1621 cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc
     1681 acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa
     1741 gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt
     1801 gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt
     1861 tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat
     1921 gttcttcgcc acttccagca gcaggtcatc aggggtgatt tctggatcgt agataaaggt
     1981 caggttcggt gaaacctgct tcaactctgc atctgcacgt aatatcgcac gggtgatggg
     2041 cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta
     2101 acgccagaaa cgttttattc gaatatcgat ctcgtcttgt gttagaattc taacatacgg
     2161 ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg
     2221 gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc
     2281 cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt
     2341 gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc
     2401 ctcggcaggc agctgcggat aaggcagatt gttttctgct tccagtgcca gaaaatggcg
     2461 cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc
     2521 attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta
     2581 agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt
     2641 gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc
     2701 ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg
     2761 caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca
     2821 gcctgcgtgc actgaaatta atggacctga ccaccctgaa tgacgacgac accgacgaga
     2881 aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc gctatctgta
     2941 tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc accccggaaa
     3001 tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa atcgcgctgg
     3061 cagaaacccg tgcggcaatc gcctacggtg cagatgaagt tgacgttgtg ttcccgtacc
     3121 gcgcgctgat ggcgggcaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg
     3181 cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg
     3241 aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac ttcatcaaaa
     3301 cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag
     3361 tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accggcgggc ggcgtgcgta
     3421 ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgtttggt gctgactggg
     3481 ctgatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ctgaaagcgc
     3541 tgggtcacgg cgacggtaag agcgccagca gctactaagt aagatgcttt acgcctgatg
     3601 cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc
     3661 atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt ctcgcacaag
     3721 aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt cgtttcttta
     3781 tcaacggtat tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca
     3841 ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt
     3901 caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacact
     3961 cgaccggcgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gcagcctgcg
     4021 gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt acgctcgaca
     4081 aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta
     4141 ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg gcggataaac
     4201 gtttctacgc gacccgtgat attaccgcaa ccgtggactc tatcccgctg atcactgcct
     4261 cgatcctggc gaagaaactg gcggaaggtc tggatgcgct ggtgatggac gtgaaagtgg
     4321 gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg
     4381 gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg aatcaggtac
     4441 tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg
     4501 agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgctgatct
     4561 ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg gtgctggaca
     4621 acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccgaccgact
     4681 tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg
     4741 ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa
     4801 tgggcggtgg acgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata
     4861 tggcgcgtct gggcgaccag gtagacggtc agcgtccgct ggcggttatc cacgcgaaag
     4921 acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata
     4981 aagcaccgga aagcacacca actgtctatc gtcgtatcag tgaataacgg tatactgatc
     5041 tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg
     5101 tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg
     5161 ctgacaccct gggtcatatc gcagaagcct gtgccaaagg cgaagctgat aacggtcgta
     5221 aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca cacgaaggtt
     5281 ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc gcgtacgcat
     5341 gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atcgccggtg
     5401 tcccggttct gtttgagtgg ggatatttct ctgatcacga aaacagcttc ccgcaagagc
     5461 tgctggataa actggtcgaa cgcgctaatc tgccgggtta cctcggtaac tgccactctt
     5521 ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt
     5581 tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc tttggcctgg
     5641 ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca
     5701 tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc cagcgtaccg
     5761 gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg
     5821 atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc cgacatctac gccaactgcg
     5881 gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag
     5941 agatgaaaga agcgggtgat aacactatcg tcttcaccaa cttcgttgac ttcgactctt
     6001 cttggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg ttcgaccgcc
     6061 gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc actgctgacc
     6121 acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg
     6181 tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc gcggatatcg
     6241 gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct
     6301 gataatgggt gcggcttgat gccctcaccc cggccctctc ccacagggag agggtgaaaa
     6361 catgtagata aggataaaac aatggctacc ccacacatta atgcagaaat gggcgatttc
     6421 gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcaa agtatattgc tgaaactttc
     6481 cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac cggtacttac
     6541 aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg ctccatctac
     6601 accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg ttcctgtggc
     6661 gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc ctgcaccgat
     6721 tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc tgactttgac
     6781 atggtgcgta acgcggtaga cgcggctaaa gcactgggcg ttgatgctcg cgtgggtaac
     6841 ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt gatggaaaaa
     6901 tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtggc tgcagagttt
     6961 ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca cgagcagacc
     7021 actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact ggaatccgtt
     7081 ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gaacgtcgtg ttaagccgga
     7141 gcaggagact gctccggctt tttagtttct attcatcaat agcaactgat ttatagattt
     7201 actattattt ttcatatgga tagaaaataa aatccattca agtatctatt cacgaatcta
     7261 ttcatttatg ttcagccgtc atccaggaag ggcatatcag caactctggt ggacaaccat
     7321 aaatttctgc cattttgctt ttgatgtttg agctaagccg ttccgtcttt tcgatctgtt
     7381 ttagagaatt aatcgagata cctaacattt tacttacttc agcaagcgat aatttatgcc
     7441 tgatacgcac ggcggctagc caggaaatac gctggcttaa cattaaattg ataatctccc
     7501 gctcctggtt ttccagatcg atcatattcc cttcctttgg tattttaatc agcgagttac
     7561 cttaccgccc ccgctatcca cgtcgacaac tcccgtagct ctttttcctg gtccgggaag
     7621 tcaaccaaca gcgcttcgca ctcttgttgc agcatatccg cgcggtacag gcagccttgc
     7681 agtcgcccgg cgagggcttc cagcggcgcg gggttgaggc tgtcggtaaa cacctgggcg
     7741 cgggtgatat ggcctttttc aacgtcgaaa tgcagttcca cgccgcccca gctaaagcgt
     7801 tcatccagca gatgcgagaa tgccggagcc tgaccgaagt tccattccca gctactctga
     7861 cgggcaaagg tttcggcgaa gtttggcaag tctggcgttt tgtccgggga gatgatttcc
     7921 gcttctacac gctcgccata atgggcgaaa aaggccttgg ttatgacctc gcaaacctgc
     7981 tcatgggtga tccccggcag cagctcggtg aggttggtca cgcgggaacg cactgaggta
     8041 atgcctttcg ccgccagttt ctttttatcc ggattgagat agtttgccag gcggctaagg
     8101 tcggcattga gcagcaaagt gccgtggtgg aagccacgat ctttggtttc gcgataggcc
     8161 gagcctgaga ctttgcggtc gccttcgact gttttcacca ccagatcgtt acgcccggac
     8221 gcttcggcgc tgacgccgag cgcgttcagc gcattgagca caatcgacgt agagatggtt
     8281 ttatcgtact ccggcttgcc agccataaag gtaaagcagg tattgccgag atcgtggaac
     8341 accgcgccgc caccgctact gcgccgcgcc aggcggacgt tatcttcttc catccgccgg
     8401 gtattacact ctttccacgg gttctgcgcg cgaccaatca ctaccgtgtc ggcattgcgc
     8461 cagagaaaca gaacgcgctg cgtggcgggc atttggcgaa aaatacactc ttccaccgcc
     8521 aggttaaacc acgggtcgta agagtcagag atgagcaggc gtaatgtgga cataacgatt
     8581 tcctttcttg tagtaatggg tcactctttt ttctcgcttt cttcctcttc cggcaccggt
     8641 ttgctggcgg ttaacaggaa gggcgattgc tgccagcggg tgcgtttacc ctgtagcagc
     8701 gtgcgggtca gcactacgcc gattgccagt gagagcagca acatcaggcg taaaatgtta
     8761 gtggtgttat ccacctgttg ggcttcggtg gcgagcgtat gggtgtcgag tgtcaggcgc
     8821 agatagccga gcggtccgtt tttacccgca attggctcga caatctgctg gttaaaatag
     8881 ccgcctgctt ttttaccgtc gagcgccagc cggtcgcgca cttcgacgct ttcgcctgaa
     8941 cgggcgataa gatcgccttg ttcgtcatac acacccgcgt cgaggatgcg gctttcatcc
     9001 gttaactgat cgaggatcgc ctgaatgcgt ttttcatctg gtgagtcggt acgcatcagc
     9061 ggtgcaacgt tcagcgtcac ctgacgcgcc agggtgcggg ccagttcttc cagctgtgga
     9121 ttacgctgtc gctggtggtt ttgactaaac catgacgccc cctgcatcag tgccactaac
     9181 aaggcaagac agaacaggac aatcactgcc cgatgcagcc ggaatttcag ttttgtgcga
     9241 gccatcttcc accctttgaa aatttgagac ttaatgttgc cagaagcaat ggatacaagg
     9301 tagcctcatg cgttattttc cctgcttcga acgattttac aggagcctta atgcctaaca
     9361 ttacctggtg cgacctgcct gaagatgtct ctttatggcc gggtctgcct ctttcattaa
     9421 gtggtgatga agtgatgcca ctggattacc acgcaggtcg tagcggctgg ctgctgtatg
     9481 gtcgtgggct ggataaacaa cgcctgaccc aataccagag caaactgggc gcggcgatgg
     9541 tgattgttgc cgcctggtgc gtggaagatt atcaggtgat tcgtctggca ggttcactca
     9601 ccgcgcgcgc tacgcgcctg gcccatgaag cgcaactgga tgtcgcgccg ctggggaaaa
     9661 tcccgcacct gcgcacgccg ggtttgctgg tgatggacat ggattccacc gccatccaga
     9721 ttgaatgtat tgatgaaatt gccaaactgg ccggaacggg cgagatggtg gcggaggtaa
     9781 ccgaacgggc gatgcgcggc gaactcgatt ttaccgccag cctgcgcagc cgcgtggcga
     9841 cgctgaaagg cgctgatgcc aatattctgc aacaggtgcg tgaaaatctg ccgctgatgc
     9901 caggcttaac gcagctggtg ctcaagctgg aaacgctggg ctggaaagtg gcgattgcct
     9961 ccggcggctt tactttcttt gctgaatacc tgcgcgacaa gctgcgcctg accgccgtgg
    10021 tagccaatga actggagatc atggacggta aatttaccgg caatgtgatc ggcgacatcg
    10081 ttgacgcgca gtacaaagcg aaaactctga cccgcctcgc gcaggagtat gaaatcccgc
    10141 tggcgcagac cgtggcgatt ggcgatggag ccaatgacct gccgatgatc aaagcggcag
    10201 ggctggggat tgcctaccat gccaagccaa aagtgaatga aaagacggaa gtcaccatcc
    10261 gtcacgctga cctgatgggg gtattctgca tcctctctgg cagcctgaat cagaagtaat
    10321 tgcctgcccg tcatcctgcg ggcggcacag cattaacgag gtacaccgtg gcaaaagctc
    10381 caaaacgcgc ctttgtttgt aatgaatgcg gggccgatta tccgcgctgg caggggcagt
    10441 gcagtgcctg tcatgcctgg aacaccatca ccgaggtgcg tcttgctgcg tcgccaacgg
    10501 tggcgcgtaa cgagcgtctc agtggctatg ccggtagcgc cggggtggca aaagtccaga
    10561 aactctccga catcagcctt gaagagctgc cgcgtttttc caccggattt aaagagttcg
    10621 accgcgtatt aggcggcggc gtggtaccgg gaagtgccat tctgattggc ggtaacccag
    10681 gcgcggggaa atccacgctg ttgctgcaaa cgctgtgcaa actggcccag cagatgaaaa
    10741 cgctgtatgt caccggcgaa gagtcgctgc aacaggtggc aatgcgcgct catcgccttg
    10801 gcctgccgac tgacaatctc aatatgttgt cggaaaccag catcgagcag atctgcctga
    10861 ttgctgaaga agagcaaccg aagctgatgg taattgactc catccaggtg atgcatatgg
    10921 cggatgtaca gtcatcgcct ggtagcgtgg cgcaggtgcg tgaaacggcg gcttatttga
    10981 cacgcttcgc caaaacgcgc ggtgtggcga ttgtcatggt tggacacgta accaaagatg
    11041 gctcgctggc gggccctaaa gtgctggaac actgtatcga ctgttcggtg cttttggatg
    11101 gcgatgccga ctcccgtttt cgcaccttgc gcagccataa aaaccgcttc ggcgcggtga
    11161 atgagctggg cgtcttcgcg atgaccgaac aggggctgcg tgaagtcagc aacccttcgg
    11221 caattttctt aagtcgcgga gatgaagtga cctccggcag ctccgtgatg gtggtgtggg
    11281 aaggaacgcg tccgttgctg gtggagattc aggcgctggt cgatcactcg atgatggcga
    11341 atccgcgccg cgtggcggta ggtctggagc aaaaccgtct ggcaatcctg ctggctgtct
    11401 tgcaccgtca cggtggtctg caaatggccg atcaggatgt atttgtgaac gtggtcggtg
    11461 gtgtgaaggt aaccgaaacc agtgccgact tagcgttact gctggcgatg gtttccagcc
    11521 tgcgtgacag accgctaccg caggatctgg tggtgtttgg tgaagtcggg ctggcagggg
    11581 agatccgccc ggtgcccagc ggtcaggagc gaatctctga agcggcgaaa cacggttttc
    11641 gccgggcgat tgttccggcg gctaacgtgc cgaaaaaagc gccggaaggg atgcagattt
    11701 ttggcgttaa aaaactctcc gacgcgctta gcgtgttcga cgacttataa tgagagatac
    11761 ggagggagat atgtcgtcat ttgattacct gaaaactgcc atcaagcaac agggctgcac
    11821 gttacagcag gtagctgatg ccagcggtat gaccaaaggg tatttaagcc agttactgaa
    11881 tgccaaaatt aaaagcccca gcgcgcaaaa gctggaggcg ttgcaccgtt ttttggggct
    11941 tgaatttccc cggcagaaga aaaccattgg tgtcgtattc ggtaagttct acccgctgca
    12001 taccggacat atctacctta tccagcgcgc ctgtagccag gttgacgagc tgcatatcat
    12061 tatgggtttt gacgataccc gcgaccgcgc gttgtttgaa gacagcgcca tgtcgcagca
    12121 gcccacggtg ccggatcgtc tgcgctggtt attgcaaact tttaaatatc agaaaaatat
    12181 tcgcattcat gctttcaacg aagagggcat ggagccgtat ccgcatggct gggatgtgtg
    12241 gagcaacggc atcaaaaagt ttatggctga aaaggggatc cagccggacc tgatctacac
    12301 ctcggaagaa gccgatgcgc cacagtatat ggaacatctg gggatcgaga cggtactggt
    12361 cgatccgaaa cgtaccttta tgagtatcag cggtgcgcag atccgcgaaa acccgttccg
    12421 ctactgggaa tatattccta cggaagtgaa gccgttcttt gtgcgtaccg tggcgatcct
    12481 tggcggcgag tcgagcggta aatccaccct ggtaaacaaa cttgccaata tcttcaatac
    12541 caccagtgcg tgggaatatg gtcgcgatta tgtcttttca cacctcggcg gtgatgagat
    12601 cgcattgcag tattccgact acgataaaat cgcgctgggc cacgcacaat acattgattt
    12661 tgcagtgaaa tacgccaata aagtggcgtt tattgacact gattttgtca ctacccaggc
    12721 gttctgcaaa aagtacgaag ggcgtgagca tccgttcgtg caggcgttga ttgatgaata
    12781 ccgtttcgat ctggtgatcc tgctggagaa caacacgccg tgggtggcgg atggtttacg
    12841 cagcctcggc agttcggtgg atcgcaaaga gttccagaac ttgctggtgg agatgctgga
    12901 agagaacaat atcgaattcg tgcgggttga agaggacgat tatgacagcc gtttcctgcg
    12961 ctgcgtggag ctggtgcggg agatgatggg ggagcagaga taaccgcgat gaaacggctc
    13021 aaaggcgagg tataaaataa gttttttgat gagttgaaaa cctttctgga agaggctgtc
    13081 gagattaaac aaggtttgaa aaaacctgca cgggtgaccc gctacgaaat tgcggatact
    13141 aaggctgttg taggccggaa aaggcgttca cgccgtatcc ggcattcggt gctcaatgcc
    13201 tgatgcgacg cttaccgcgt cttatcaggc ctggagattc acaacgtatt gaaatttctt
    13261 ggtttgtagg ccggataagg cgttcacgcc gcatccggca ttttacgcat tacttcgcaa
    13321 tacgcttgta cttgatacgc ttcggctcca gtgcgtctgc gcccagcgtg cgtttcttgt
    13381 actcttcgta ctcagtaaag ttaccttcga agaactcaac tttaccttca tcctggtagt
    13441 ccaggatgtg cgtggcgata cggtcgagga accaacggtc gtgcgagata accatcgcac
    13501 agcccgggaa ctccagcagg gcgttttcca gcgcgcgcag ggtttcgata tccaggtcgt
    13561 tggttggttc gtcgagcagc agcatgttgc cgccaacctg cagcagcttc gccagatgca
    13621 gacgaccgcg ctcaccaccg gaaagttcac caacgcgttt accctgatca acccctttaa
    13681 agttaaagcg gccaacgtag gcgcggcttg gcatctcggt gttgccgatc ttcataatat
    13741 ccagcccgcc ggaaacttct tcccaaacgg ttttgctgtt atccattgag tcacggaact
    13801 gatcaaccga tgccagtttc accgtttcac ctaaagtgat ggtgccgctg tccggctgtt
    13861 cctgaccaga gatcatacgg aacagggtcg atttacccgc gccgttcgga ccgatgatcc
    13921 cgacgattgc ccctttcggg atcgagaagc tcaggtcatc aatcagcagg cgatcaccgt
    13981 aggatttacg caggttgctg acttccagca ctttatcgcc cagacgcggt ccaggtggaa
    14041 taaacagttc gttggtttcg ttacgtttct gatattcggt gctgttcagc tcttcaaagc
    14101 gtgccagacg tgctttacct ttcgactggc ggcctttagt tccctgacgt acccactcca
    14161 gctctttctc gatcgactta cgacgcgccg cttcttgtga agcttcctgc gccaggcgct
    14221 gatctttctg ctccagccag gaggagtagt tgccttccca cggaatacct tcaccgcggt
    14281 caagttcgag gatccagcct gcaacgttat cgaggaagta acggtcgtgg gtaatcgcca
    14341 ccacggtgcc ctcgaagtcg tgcaggaagc gttccagcca ggccacggat tcggcatcca
    14401 ggtggttggt cggttcgtcg agcagcagca tgtctggttt ttccagcagc aggcggcaca
    14461 acgctacgcg acgacgctca ccaccggaga ggttagcgat tttcgcgtcc cagtccggca
    14521 gacgtagcgc atccgccgca cgctccagct gtacgttcag gttatgaccg tcgtgagcct
    14581 gaatgatctc ttccagacgg ccttgttcag cggccagctt gtcaaaatcg gcatccggat
    14641 cggcgtacag cgcatacact tcatccaggc gtttcagggc gttaaccact tcagaaaccg
    14701 cttcttcaat ggactcacgc acggtgtgtt ccgggttcag ctgcggttcc tgcggcaggt
    14761 aaccaatctt gatgtctggc tgcggacgcg cttcaccttc gatgtcttta tcaatgcccg
    14821 ccataatgcg cagcagggta gacttacccg cgccgttcag acccaggaca ccaatttttg
    14881 ccccagggaa gaaactcaga gagatgtttt tcaaaatatg acgtttcggc ggaacaactt
    14941 tgccgacacg atgcatggta taaacgaatt gagccacgtt ggacttcgcc tctatgttta
    15001 tcgtgatgat gagttttcaa aggcgaagtg tagccttttt ccccgcctaa tcccagttgg
    15061 tcgatcacct tcgcagtaaa agtaaaaaag tgtccgtaac gtggcgtaaa cggcaatgac
    15121 tggttagcat aaatctatta cgcggcatga cgctgcattg atgtatttac acttagagga
    15181 tgcgcttgtg gaaaaagcca aacaagttac ctggcggctg ttggctgccg gtgtctgtct
    15241 gctgacggtc agcagcgtgg cgcgagccga ctcactggat gagcagcgta gccgttacgc
    15301 gcaaatcaag caggcctggg ataatcgaca aatggatgtg gtcgaacaaa tgatgcctgg
    15361 actgaaggat tatccgcttt atccctacct ggaataccgc cagatcaccg atgatctgat
    15421 gaatcaaccg gcggtgacgg tcactaactt tgttcgcgct aaccccacgc ttcctcccgc
    15481 tcgcacgctg caatctcgtt tcgtcaatga actggcgcgg cgtgaagact ggcgtggctt
    15541 gttagccttt agcccggaaa agcccggaac taccgaagcg caatgtaatt actactatgc
    15601 gaaatggaac accgggcaga gtgaagaagc ctggcaaggg gcgaaagagc tgtggctaac
    15661 cggcaagagc cagcctaacg cctgtgacaa gttatttagc gtctggcgtg cgtcaggtaa
    15721 acaagatccg ctggcttatt tagagcgtat ccgtctggcg atgaaagcgg gtaacactgg
    15781 cctggtaaca gtgctggcag ggcagatgcc tgccgattac cagactatcg cctcggcaat
    15841 catttcactg gcgaacaacc ctaatacggt actgaccttc gcgcgtacaa ccggagcgac
    15901 cgattttacc cgtcaaatgg cggcggtggc gtttgccagt gtggcgcggc aggatgctga
    15961 aaatgcacgg ctgatgatcc cgtcgcttgc ccaggcgcag cagcttaatg aagatcatat
    16021 tcaggagctg cgcgatatcg tcgcctggcg tttgatgggc aacgatgtca ccgacgagca
    16081 ggcgaaatgg cgcgatgacg ccattatgcg ctcgcaatcc acgtcgttga ttgaacgccg
    16141 tgtgcgaatg gcacttggca ccggcgatcg tcgcggcctg aatacctggc tggcgcgtct
    16201 gccgatggaa gcaaaagaga aagatgaatg gcgttactgg caggcggatt tattgctgga
    16261 acgcggacgt gaagctgaag cgaaagagat tttgcatcaa ctcatgcaac agcgtggttt
    16321 ctacccgatg gttgcagcac aacgcatcgg agaagagtat gagctgaaga ttgataaagc
    16381 gccgcagaat gttgacagcg ccctgactca ggggccggag atggcgcgcg tgcgcgagtt
    16441 gatgtactgg aatctcgata acaccgcgcg tagcgagtgg gccaatctgg tgaagagcaa
    16501 gtcaaaaaca gagcaggctc aactggctcg gtatgctttc aataaccaat ggtgggatct
    16561 tagcgttcag gcaacgatcg ccgggaagct ttgggatcat ctggaagagc gattcccgct
    16621 ggcttacaac gatcttttca aacgttacac cagtgggaaa gagatcccgc aaagctatgc
    16681 gatggcgatt gcccgtcagg agagcgcctg gaatccgaaa gtgaaatcac cggtaggggc
    16741 cagcggcctg atgcagatta tgcctggtac agcgacccat acggtgaaga tgttctctat
    16801 tcctggttat agcagccctg ggcaattgct ggatccggaa acgaatatca acattggcac
    16861 cagttacctg caatatgttt atcagcagtt tggcaataac cgtattttct cctcagcagc
    16921 ttataacgcc ggacctggac gggtgcgaac ctggcttggc aacagcgccg ggcgtatcga
    16981 cgctgtggca tttgtcgaga gtattccgtt ctccgagacg cgcggttatg tgaagaacgt
    17041 gctggcttat gacgcttact accgctattt catgggggat aaaccgacgt tgatgagcac
    17101 cacggaatgg ggacgtcgtt actgatccgc acgtttatga tatgctatcg tactctttag
    17161 cgagtacaac cgggggaggc attttgcttc ccccgctaac aatggcgaca tattatggcc
    17221 caacaatcac cctattcagc agcgatggca gaacagcgtc accaggagtg gttacgtttt
    17281 gtcgacctgc ttaagaatgc ctaccaaaac gatctccatt taccgttgtt aaacctgatg
    17341 ctgacgccag atgagcgcga agcgttgggg actcgcgtgc gtattgtcga agagctgttg
    17401 cgcggcgaaa tgagccagcg tgagttaaaa aatgaactcg gcgcgggcat cgcgacgatt
    17461 acgcgtggat ctaacagcct gaaagccgcg cccgttgagc tgcgccagtg gctggaagag
    17521 gtgttgctga aaagcgattg att
//
LOCUS       DAZFRJ010000086        17387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000086 DAZFRJ010000000
VERSION     DAZFRJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.086"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..1099
                     /gene="allD"
                     /locus_tag="ACHKUN_002643"
     CDS             50..1099
                     /gene="allD"
                     /locus_tag="ACHKUN_002643"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO6660879.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1121..2356
                     /gene="allC"
                     /locus_tag="ACHKUN_002644"
     CDS             1121..2356
                     /gene="allC"
                     /locus_tag="ACHKUN_002644"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO6660880.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2367..3152
                     /gene="allE"
                     /locus_tag="ACHKUN_002645"
     CDS             2367..3152
                     /gene="allE"
                     /locus_tag="ACHKUN_002645"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO6660881.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3281..4426)
                     /gene="glxK"
                     /locus_tag="ACHKUN_002646"
     CDS             complement(3281..4426)
                     /gene="glxK"
                     /locus_tag="ACHKUN_002646"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO6660882.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4448..5748)
                     /gene="ybbY"
                     /locus_tag="ACHKUN_002647"
                     /pseudo
     CDS             complement(4448..5748)
                     /gene="ybbY"
                     /locus_tag="ACHKUN_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(5805..7166)
                     /gene="allB"
                     /locus_tag="ACHKUN_002648"
     CDS             complement(5805..7166)
                     /gene="allB"
                     /locus_tag="ACHKUN_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO6660883.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7226..8680)
                     /gene="ybbW"
                     /locus_tag="ACHKUN_002649"
     CDS             complement(7226..8680)
                     /gene="ybbW"
                     /locus_tag="ACHKUN_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO6660884.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(8849..9727)
                     /gene="glxR"
                     /locus_tag="ACHKUN_002650"
     CDS             complement(8849..9727)
                     /gene="glxR"
                     /locus_tag="ACHKUN_002650"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6660885.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9827..10603)
                     /gene="hyi"
                     /locus_tag="ACHKUN_002651"
     CDS             complement(9827..10603)
                     /gene="hyi"
                     /locus_tag="ACHKUN_002651"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO6660886.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(10616..12397)
                     /gene="gcl"
                     /locus_tag="ACHKUN_002652"
     CDS             complement(10616..12397)
                     /gene="gcl"
                     /locus_tag="ACHKUN_002652"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO6660887.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(12487..13302)
                     /gene="allR"
                     /locus_tag="ACHKUN_002653"
     CDS             complement(12487..13302)
                     /gene="allR"
                     /locus_tag="ACHKUN_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO6660888.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(13380..13862)
                     /gene="allA"
                     /locus_tag="ACHKUN_002654"
     CDS             complement(13380..13862)
                     /gene="allA"
                     /locus_tag="ACHKUN_002654"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO6660889.1"
                     /translation="MKLQVLLLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            14092..15018
                     /gene="allS"
                     /locus_tag="ACHKUN_002655"
     CDS             14092..15018
                     /gene="allS"
                     /locus_tag="ACHKUN_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO6660890.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            15087..16181
                     /gene="mnmH"
                     /locus_tag="ACHKUN_002656"
     CDS             15087..16181
                     /gene="mnmH"
                     /locus_tag="ACHKUN_002656"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO6660891.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            16297..16662
                     /gene="ylbG"
                     /locus_tag="ACHKUN_002657"
                     /pseudo
     CDS             16297..16662
                     /gene="ylbG"
                     /locus_tag="ACHKUN_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17183..>17387)
                     /locus_tag="ACHKUN_002658"
     CDS             complement(17183..>17387)
                     /locus_tag="ACHKUN_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660892.1"
                     /translation="KTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFV
                     GEINVTHYFITNIGAGLPDACAE"
BASE COUNT         4552 a         4553 c         4148 g         4134 t
ORIGIN      
        1 gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa ggaagacaga tgaaaatcag
       61 tcgggaaaca ctccaccagc taattgagaa taaactctgc caggctgggt taaaacgtga
      121 gcacgctgca accgtggctg aagtattggt ttacgccgat gccagaggga tccactctca
      181 tggcgcggtg cgcgtggaat actacgcgga acgcatttca aaaggcggca ccaaccgcga
      241 accggagttt cgtcttgagg aaaccgggcc gtgctcggca attttacatg ccgacaatgc
      301 cgccggacag gtcgcggcga aaatgggtat ggaacatgcc atcaaaaccg cccagcaaaa
      361 tggcgttgcg gtggtcggta tcagccggat gggtcacagc ggcgcaatct cttattttgt
      421 gcagcaggca gcccgcgccg gattaattgg tatttcgatg tgccagtccg atccaatggt
      481 ggtgccgttt ggcggcgcgg aaatttacta cggtactaac ccactggcct ttgccgcgcc
      541 gggagaaggc gacgagatcc ttacctttga tatggcgact accgtacagg catggggaaa
      601 agtgctcgac gcccgctcgc gtaatatgtc tatcccggat acctgggcgg tcgataaaaa
      661 cggtgcacca acaaccgatc cgttcgcggt acatgctctg ctccccgccg ccgggccaaa
      721 agggtatggc ctgatgatga tgattgacgt cctctcaggc gtcttactcg gcttaccgtt
      781 cgggcgacag gttagttcga tgtatgacga tttacacgcc gggcgtaatt tggggcaatt
      841 acatatcgtt attaacccga actttttctt ctccagcaaa ttatttcgtc aacatcttag
      901 ccagaccatg cgcgaattaa atgccattac ccccgcgccc ggttttaatc aggtttatta
      961 tcccggacag gatcaggata ttaaacaacg caaagccgcc gtcgaaggca tcgaaattgt
     1021 tgatgatatt taccagtatt taatttccga cgcgctttat aacacgtcat acgaaacgaa
     1081 aaatcccttt gcgcaataat tattgagaca ggactttctt atgattacac attttcgtca
     1141 agccatagaa gaaacgctgc cctggctttc ctcttttggc gctgacccaa cgggtggtat
     1201 gacccgttta ctttattcgc cggaatggct ggaaactcag cagcaattta aaaaaagaat
     1261 ggcagcaagc gggctggaaa cacgtttcga tgaagtgggg aatttatatg gtcgcctgag
     1321 tggcaccgaa tatccacaag aagtggttct gagcggttcg catatcgata ccgtggttaa
     1381 cggcggtaac ctcgacgggc aattcggcgc gctggcggcg tggctggcaa ttgactggct
     1441 gaaaacgcaa tacggcgcgc cgctacgtac ggtcgaagtg gtggcgatgg cagaagaaga
     1501 aggcagccgc ttcccgtatg tcttctgggg cagtaaaaat atctttgggc tggcgaatcc
     1561 tgacgacgtg cggaatatct gtgatgccaa aggaaatagt tttgtcgatg cgatgaaggc
     1621 ttgcggattt actcttccga acgccccact aactccgcgt caggatatta aagcctttgt
     1681 tgaactgcat attgaacagg gctgtgtgct ggaaagtaat gggcaatcaa ttggcgtggt
     1741 gaatgcaatt gtcgggcagc gtcgttatac ggtaacgctg aacggcgaat caaaccatgc
     1801 aggcaccacg ccgatgggtt atcgtcgtga tacagtttac gctttcagtc gcatttgcca
     1861 tcagtcggtc gaaaaagcga aaaggatggg cgatccgctg gttctgacct ttggcaaagt
     1921 agagccgcgc ccgaatacgg taaatgtggt gccgggtaaa accacgttca ccattgattg
     1981 tcgtcatacc gacgctgccg tgctgcgcga tttcacccaa cagttagaaa acgacatgcg
     2041 ggcgatttgc gatgaaatgg acattggtat tgatatcgat ttatggatgg acgaagaacc
     2101 cgtgccgatg aataaggagc tggtcgccac cctgacagaa ttgtgtgaaa gagaaaaact
     2161 gaattaccgg gtgatgcaca gtggtgccgg gcacgacgcg caaattttcg cgcctcgcgt
     2221 accaacctgc atgattttca ttcccagcat caacgggatc agccataacc cggcggaacg
     2281 caccaatatt accgaccttg ccgaaggggt caaaacgttg gcactcatgc tttatcaact
     2341 tgcctggcag aaataaggag tcataaatgg gatatttaaa taacgtcacc ggttaccgcg
     2401 atgatttact ggctaaccgt gcgattgtta aacacggtaa tttcgcactg ttaaccccgg
     2461 atggtctggt aaaaaatatt attccgggct ttgaaaattg tgacgcgaca atcctctcca
     2521 cgccaaagct gggtgcctct tttgttgatt atctggtcac actgcatcaa aacggtggca
     2581 accaacaggg cttcggtggc gaaggcattg aaacgttcct gtatgtgatc tctggaaata
     2641 tcactgccaa agccgaaggc aaaacatttg ccttaagcga aggtggctat ctttattgcc
     2701 caccaggctc cttaatgacg tttgttaacg cccaggccga agacagccaa atctttttat
     2761 ataagcgccg ctatgttccg gtagaaggcc atgcaccgtg gctggtttct ggtaatgcca
     2821 gcgaactgga acgcattcac tatgaaggta tggatgatgt gatcctgctg gatttcctgc
     2881 ccaaagagtt aggttttgat atgaacatgc atatcctctc ttttgcgcca ggtgccagcc
     2941 acggttatat cgaaacacac gttcaggaac atggtgccta tattctttcc ggtcaggggg
     3001 tttataacct cgacaataac tggatcccgg tgaaaaaagg cgattacatc tttatgggcg
     3061 cttattcttt acaggctggt tatggcgtag ggcgtggtga agcgttcagc tatatttact
     3121 cgaaagattg caaccgcgac gtagagattt aagtcatacc actcgcctga ttatttttag
     3181 ctatgttgga ttttgccacg gtttataccg tggctttttt tgtagtaaat tcaggtaaaa
     3241 aaagatgcga aataaatcgc atccgcttta aaggtaaggg tcagttttta attccctgac
     3301 ctattttaat ggcgcaggca atatttcgcg cgctgttgaa gagattggtt tcaccgctgg
     3361 cgagcacttc ggctaaaggt gccagacgag gcaaaatgct gaataccgcg tcaatgccgt
     3421 actggtgcac cacttccacg ccatcaccca atacgccagc aatcccaatc accgggacat
     3481 taaactgctt cgccaccgac gcaacgccca gcggcgcttt accgcctgcc gtttgcgagt
     3541 caatgcgccc ttccccggtg atcaccagtg ctgcgccctg caccgcctgc tccaggttga
     3601 ccgcccgcag tacaatctca atacccggtt taatatccgc attgaggaat accgccgccg
     3661 caatacccat accgcccgca gcgccgccgc ccgccatctg gcagacatta atttcagttt
     3721 gctgttgtaa aacacgggcg taattttgca gcccctgttc aagttcttcg accatctccg
     3781 gcgttgcccc tttttgcggg ccaaacaccg ccgccgcgcc gcgtgcccct accagcgggt
     3841 tatcaacgtc acatgctact tcaatatggc aattcgccag acgcggatcg cattcatcca
     3901 tctcaatgct tgccacgcgc gctaaattac cgccgttagc cgccagcacc tggccgtcgg
     3961 catcaaggaa acgcacacca agcgcctgag ccatgcccat accgccgtcg accgtcgcac
     4021 tgccgccaat gccgagaata atatggcgaa tgccgttatc cagcgcatga cgaattaact
     4081 cccccgtacc aaaactggag gccagcaacg gattacgctt ttcaggcgcg accagcatca
     4141 ggccacttgc tgccgccatc tcaatcaccg ccgttttccc gtcgccggta aggccataaa
     4201 aagcattcac tgtttcgccc atcggcccgc agacttcaag cgtcacgatg ttgccgcccg
     4261 tcgcggcgac catcgcatcc accgtccctt cgccgccatc cgctatcggc aaacagatat
     4321 agttcgcatc gggaaagagg gtcgaaaacc cggctttaat tgcctgacaa catttttctg
     4381 cacttaagct ctctttaaaa gagtctggcg caatgacaat cttcattatc aatccttttt
     4441 caacaaatta ttcgatgcgt tcccacggaa taagattatc cataagaaca gccagtaaaa
     4501 ttccgaccag taagccgtta ctgagcagag gacgcagcgt taatggaagg tcttgcagat
     4561 acacaggcgg taatgccatt aaaaatatgc cgacaaataa cggcaatgcg agtcgataaa
     4621 tattgcgagc ggtaaaggtt atttgctggc taaacaccag cgcggaaaag agtaaaggca
     4681 gataagaaac cagcatgacc gcgctactca cgggtaaagg gatactgcaa aacagtcgtg
     4741 tgagtgccgg aaccagcgcc accagcaggc aaataacgct gccataaata aacgaacgcc
     4801 gcgtgtaatc gcctgtctgg gttaataaac caatggatga aacaaacggt gaaaatggaa
     4861 ttaccgccag cggtacggtt atcagcgtca taaatccggt cgccacaaag ctgcgacgat
     4921 aacgcgtatt cccgcgccct gctgcggata aaaaacatcc gtgccccgaa tcgcaccgta
     4981 ggtattgctg atattcacca gacctgtaat caccgccgtc agaataattc ccggagacaa
     5041 agcacctcca ctgccgagcg ggaaccactg ccagtgcaac tcaccggaga gcgagtgcga
     5101 agaaggaaag caaaagtacc acaacaacca gccggttatg gtgccgacca gcaggccata
     5161 acgggcaaaa cgttgcggca ggaagataat catccccagt accaggcaca tcaccgccac
     5221 cgagagcgcg aacggcggta actgaatttt aaaatttggg tcggctatgc caaacggcag
     5281 cccgagcata cctttgaaaa agatagtggt cagctgcgcg cccagcatca acataaacaa
     5341 gaccatcacc gacggcgtaa ataaccgtgc caggcgatgg cctaatccgc taaaaccaat
     5401 caacatcgtc agcacgccgg agagcgcaat ccccaccgcc aggctggtag cgatatcgtt
     5461 gatcggtgtc ccgcgcgatg cttcgccaag ggtgatagta aggatggttc cccaccacag
     5521 gccacccggc ccttccataa tagcgcgacg atgaccgcag aacgcctgag cgaagcaggc
     5581 cagtgcggta gcaagaaaag catattgcgt gagcgtaagc aggctacttt gcggcaactg
     5641 aaaagcagaa agtagcgtag gaggaaccac aaccgtgttg caaaaaataa agaaaaacca
     5701 ctgaaatcct gataacaggc tttcgcggct cactgcaaaa ttgaacataa acacacctta
     5761 accggagaat gcccgccgca aggacgggca ttgcaggggc cagattactg ctgatgttta
     5821 aggataaatt gacctttcgg cgcaacaggg aagccctgtt caatgtcgta aatcacatca
     5881 ccacgtaaga tggttttcgt gatacgcgcg ccaatggtac ggccaacata cgggctgact
     5941 ttgtggcgat attccagatc gtcattggta agaacatagc tgctattcgg ctgaatgaag
     6001 acgaagtcgg catcttttcc tggggcgata cggccttttt gctgcagacc gaaaatatct
     6061 gctgcgttag tcgccattaa tttgccgaac attggcagag acattccgcg tttctgtacc
     6121 gcttcatcga acatcacgtc catacagttt tgcagaccgg caataccgcc ccatgcttcc
     6181 atgatgttgc cggctttcat ttccggaggg catggtgagt ggtcggaaac caggcagtcg
     6241 atttcaccgt taaacagttt ttcccacatg cctttctggt tttccagatc gcggatcggc
     6301 ggtgaacact tcgccagagt gccgatttct tcgaactgat cggtatccag tacaaagtaa
     6361 tgcgggcagg attcacaagt aacgtcctga ccttcctgac gtgcacgagt cacttcttca
     6421 acgccttccg ggctgctgat atggcaaacg tgcagacggc aaccggcaac tttcgccaga
     6481 tacagcacgc ggcgaatcgc ttccacttcg gtaaataccg gacgcgaagc cacatagtca
     6541 tgggcagtta cgcgaccttc acgtttcgct tcttcgccca gtgcgtcaca gatcagcgcg
     6601 ttttcgcagt gcaccagcac cggctgcccc agttcgccca gcttctgcgc acctttgaag
     6661 aactgccagt cgtttacatc acggaagtcg ttgtcgatac cgcgatcgcc acaggtcgca
     6721 acgaagcatt tgaagccgac aacgcccact tcatccagct catgcagacg gtcgatgttg
     6781 taagacacca ggccaccgag ttgtgccgca tcgatagtca gcttgccttt agcggcatcg
     6841 aacttcagtt caattgaagc gcggtcaacc gttgcaggca gctggttgag cggcatttcg
     6901 atcatggtgg tgataccacc ttttgccgct gcgcgagtac cggtttcata accttcccag
     6961 tggctacgac ccggttcaga aatatgggtg tgcgcatcaa ccatgcccgg cgaaaccacc
     7021 agaccagacg catccataac ttcttttgca tcgcccagat cctgaccgat agcagcaatt
     7081 ttcccgcctt taacggcgat atctacaacg cgagcttcgt tttctaaaat aacggtgccg
     7141 tttttaatga ttaaatcaaa agacataaca aactccttgt aaaatacaga ttggttttta
     7201 actcaatgtt caatatcggg attaattaac ctatggtttt ttgctctcct gttttttctg
     7261 ctgttgtacg tttctttaat aaggcgtagg ccgcaaaggc gacgatgacg ccgacaaacc
     7321 atgaaacacg cgataacggt tccataaagt gaataaactt accgccaaga gataaaataa
     7381 cggccaccag agttactgaa aacgcagtga ggttaaaacc gttatcgtaa tatttataat
     7441 cgccaggtgc ggtatacagt tcatcaagat taatttgtcc gcgcatcacc acaaaataat
     7501 gcgccatcat gacaccaatt accggaccaa gcattccgcc gataatatcg aggaaaagat
     7561 aaatgctgtc ctgattttcc attaatttcc acgggcagat cagcaagctg ataatactgg
     7621 caatcagtac gccgttttta taggtcagtt ttgtcggtgc aatggcggca atctgataac
     7681 cggctggaat aatattaccg gtcgcgttag tggagatagt tgtcatcaga atgaccagta
     7741 ccgcaaagaa cgaggcgaac aggctgtccc aacgctgaac aatatccagc acgttccagg
     7801 tatcagcgcc gtagtgaata ctggctccgg caataataca taccccggcg accgcaaaca
     7861 gaatataggc cacaactaaa cccagcgttt gccccagcgc ctgctcacga aacgagtggg
     7921 cgttttgcgt aaagtcggat gcgctcaccg ccggtgccgc ccagaccgca actaccgcgt
     7981 taatcaccac caggaacagg aagccaccgt tttctgcttt ctgaataccg ctcggaatgt
     8041 agtcaaagat tggaccgatc ccgaccagtg aaatcgccca aatcgccata ccgccgaaaa
     8101 cgatatagat gcacgggtta agaatggcag tgaatttatt taaaactttg ccaccgccaa
     8161 aacctatacc aacgttgacc agccagaaga ttaagaaagt aattaagccc ggtagagaaa
     8221 ggcctaacag agtgaaatca ccaccgagag ttaaaaatcc cggccagatt ttgccaatca
     8281 gaatcaagca ggccagtgac cccgcgtaac attgcaaacc aaaccacatg atggcggcaa
     8341 ttccgcccct taataatccg ggaaacagtg caccacgtac accgtaagaa gcacgcagga
     8401 tcatggcaaa aggcacaccg tatttactgc ccgcagcacc gtttaatacc attaccgcgg
     8461 caatgaaaaa ggcgctgagg ataattgcca gcataatact aaaggtagac aagccgagaa
     8521 taaaaaagcc gccgaccatc acataattgg gaacgttatg aaccgaaccc atccataagg
     8581 taaaatagtt aaatgttttc caggtccgct ggctttgcgt tttcggcaat agatcttcgc
     8641 tatagccgcg ttgctggaat agttttctct gatgttccat aaatccctct taatttcacg
     8701 tatcgaataa cgttgtttta atctagctat gaggaacaaa taaaataaaa atattatttt
     8761 taatactgaa gcaacatctt tggctgcttt cttttttaat attgctggcc gattcatttt
     8821 ctgatatcgg ccatttttat tgcgggtatc aggccagttt atggttagcc attaattcca
     8881 gcgcctgcac taacgcagag tgatccaact ggctgccacc gtttgccgcg caggtattaa
     8941 ataactcctg gcaggtcgca gtgtttggca ggttcagcgc tagcgctttc gcactttgca
     9001 gtgccaggtt gagatctttc tggtgcagcg caattttgaa acctggatta aaagtgcgtt
     9061 taatcatgcg ctcaccgtga acttccagaa tacgtgagga agcaaagccg cccatcagcg
     9121 cctggcgcac acgtaccggg tccgcaccgg cttttgaagc aaatagcagg gcttcagaaa
     9181 ccgcttcaat attgagcgcc acaataatct gatttgccac tttgcaggtt tgaccatcgc
     9241 cgttaccgcc cacgagggtg atatttttac cgagcagttc aaacagcggt ttaacacgtt
     9301 caaataccgc ttcatcaccg ccaaccataa tcgacaacgt cccttcacgc gcaccgattt
     9361 caccgccgga gactggcgca tcgagataat cgccgcccag ttcattcacc tgacgagcga
     9421 aacgcttagt ttcaatcggg gaaatggagc tcatatcaac aatggttttg cccttcagcg
     9481 aggctttggt acaaccattt tcaccgaaca gaacttcttc aacctgtggt gtatctggca
     9541 ccataataaa aatgatgtcc gatgcttccg ttacctggcg cgcagtttca acactgacgg
     9601 cacccagtga cagtaattca tcagcaaccg gaccaatggt tgtgacatgc agttgatgac
     9661 cggcacgcgc cagattaatg gccatcggtg tacccataat gcctaagcca ataaatccca
     9721 gtttcataat taacctcttt taaatttcgc ttttcctgaa ttcagacaac acgatcccct
     9781 gccaacttag cggcctaaaa aagcattgcc tgaacagcgt tacgttttaa cggtacggat
     9841 ccatccagcg taaaccggct tccgtggtgg tttggggtgt atattcacac ccaacccaac
     9901 cgttgtagtc agaattttcg attaccttaa agagataatc ataattaatt tctccggtcc
     9961 ccggttcgcc gcgatgcgga ttatcagcaa tttgcaggtg accaatttta tcggcccact
    10021 gagtcatggt gttggttaat tcaccttcca tccgctgcat gtgataaatg tcatactgaa
    10081 ttttcaaatt gcagcaacca acatcatcaa tcaatttcag cgcctgccga gttccggtga
    10141 gatggaaacc aggaatatca aaatggttaa taggttcaat cagtaataaa atatcttctt
    10201 tcatcagcat attcgcggca taacgcaggt tttctacaag cgttgcgtga atctgttcac
    10261 tgctgaaacc agccggcgtt ttaccgacca gacagttaat ttttttatta cccagcgcac
    10321 gggcataacg aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac
    10381 aggcaatacc gcgctccccc gccgcccagt cacctgccgg taaattgtgc agcgtatgtt
    10441 cgagtttatt actcgccagc acctgtttta attcttcaat gtcgtagtca taaggaaaca
    10501 taaattcaac gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat
    10561 attcaccaaa taacatcgat aaattagcag agaaacgtaa cattatttat ctcccttatt
    10621 catagtgcat gaagcaggtt tcagtcggtg cgtccgctgc gttatcggcg atatcttcaa
    10681 attccatgac gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcaagaataa
    10741 cttccacgac taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg
    10801 gcgcaatatc ttccggtttg aagacccgaa tagctttaca acctaaacct tccgctactt
    10861 ttacgtggtc gacgccgtaa ccgttcactt cactggagtt aatattttcg aacgcgagct
    10921 gtacgcagta atccatgtca aaggcgcgtt gtgactgacg aatcagcccc agataagcgt
    10981 tattgaccag tacatggatg tacggaatgt tgaactgtgc gccaacggct aattcttcaa
    11041 tcaggaactg gaagtcgaag tcaccggaaa tcgccaccac attccgttct ggatcagcag
    11101 cacacacgcc cagcgccgcc ggaatggtcc agcccagcgg gcctgcctga ccacagttga
    11161 tccagtgacg gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacaggccaa
    11221 tggtggtgac ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct
    11281 gcggtttcac cggcacgttg tcgaagtggg ttttgcgcag caaagtgcgt ttgcgctgct
    11341 ggcagtcggc gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg
    11401 ccacttcaac cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac
    11461 acagcacgcg accaatttgc gtcggctcaa tatcgatatg aacgattttg cgcccttcgg
    11521 tgtatttctc taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca
    11581 tgtcggacgc cagcagcgtt gcgttaccgt aacgatgcgc ggtttgcaga cccaccatcc
    11641 cggccatcag ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg
    11701 gaacgctggt cagttcagca aactgttgta acagtgcagc tgcgtcagca ttaattaccc
    11761 cgcccccggc aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga
    11821 tctgcatacg gctggcagca ggtttgtaga ccggcagcgg ttcgtacata tcaggatcaa
    11881 actcgatttc cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac
    11941 cagaacgcat caggtgaaat gcctgttgca acacacgagg caccagcgcc gcttcacgta
    12001 ctgtaaccgc cattttactt accggcttgg cgatagcttc gatatcaaca gcctggaaat
    12061 cttctttatg cagacgggcg cgcggagcct ggccagtaat acacagaata ggaatggaat
    12121 cagcagaagc agaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca
    12181 gacatacgcc gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaagcac
    12241 cttccacatg acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga
    12301 acggattgat tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca
    12361 catacattgc cgcgtcaacg gctctcattt ttgccatttt attccaacct ctattaattg
    12421 gaaaaatttt ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga
    12481 cagacactat ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct
    12541 gaccagctca ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat
    12601 ggagatagcg gcaacaacgc taccgacatc atcgtaaatt gctgaagcta tgcaattcag
    12661 acctacaaca tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc
    12721 cttcagcaag gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg
    12781 cagaatgatg ctcatcaact cctcttccgc cagcggataa agcaacgcct tgcccgcacc
    12841 ggaagcatgc agcggcagac gactgcccag tggcgcacac atcctgacca tcgatttaca
    12901 ctctaactga ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc
    12961 gccggaaagt aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt
    13021 atggatgtac gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaactg
    13081 actgtcctga tagacaaaat ccgctgcctg taaaaccttc agcaagcgaa aggtcgtaga
    13141 gagcggcaaa tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc
    13201 cagatattgc agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg
    13261 ctccgcctgt cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac
    13321 ctacaagtga gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt
    13381 cacgcaaaac agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg
    13441 cgatcgatgg tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg
    13501 ttacggtggt aattcactcc ctgttcgccg ttggtaataa acgcccgcag ggttgacagg
    13561 tctggtttgt cgtcacctaa cgccacgacc accacaaaca cctcaccttt catcgggata
    13621 aaggcctgag tacccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc
    13681 ggttgcgcgc ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc
    13741 aaatcgtggt aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt
    13801 tcgattacgt cgccataagc actaaaggct tcctgactta acagtaatac ctgaagtttc
    13861 atctttttgt ccttgtaaca taattccgtt caaccaccgc cgaccggtca gcttggtttc
    13921 aataacaggc aatctaaaac cttaagagaa caacatcaat acaaaagttg gaatattttt
    13981 ccaaaactag atttagtcca caaattgaac agggttatca tgtaaaaata ctgataaatc
    14041 agaggataag aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat
    14101 ccagaaacct tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca
    14161 gaacgattat gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat
    14221 accggagtag cgctgttttt ccgcacgact cgcagcgtga cgttgacagc ggctggcgag
    14281 catctacttt cccaggccag agactggctg agctggctgg aaagtatgcc aagcgagctg
    14341 caacaggtga atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctac
    14401 aacccccagg ccgtcgccca gttgctggcg tggctgaatg aacgttaccc ctttacccag
    14461 tttcacatct cccgacaaat ctatatgggc gtctgggatt cgctattata cgaaggtttt
    14521 tcactggcta tcggcgtcac gggaaccgag gcgctggcaa atacctttag tcttgatccc
    14581 ttaggatcgg tgcaatggcg ctttgtcatg gcggcggatc atccgctggc gaacgttgaa
    14641 gagccgctaa cagaagcgca gttgcggcgc tttccggcgg tcaatattga agacagcgcc
    14701 cgcaccttaa ccaaacgcgt cgcctggcga ttgcctgggc aaaaagagat tattgttcct
    14761 gatatggaaa cgaaaatcgc cgcccatctt gcgggcgttg gcattggttt tttgccaaaa
    14821 tcgctttgcc agtcaatgat cgataatcaa caactagtta gccgggtaat cccaacgatg
    14881 cgccctcctt cgccattgag tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa
    14941 gatattgtga ccttgtttac ccagcgcagg ccggaaatca gcggattttt agaaattttc
    15001 ggcaacccac gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca
    15061 agctgatttg aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg
    15121 ccctgctgat tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag
    15181 gcgcaatgcc cgccgctatc aatctgccgt taatgaataa cgatgaacgc gccgccgttg
    15241 gcacctgcta taaacagcaa ggctcagacg cagcgctggc gctggggcat aaactggtgg
    15301 cgggtgaaat tcgtcagcag cgcatggacg cctggcgggc agcgtgcctg caaaatccgc
    15361 aaggtattct ctgctgcgcc cgtggcggtc agcgctcaca tattgtgcaa agctggttgt
    15421 atgcagcggg gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg
    15481 cgattcaggc gactattgaa ctggcacaaa aaccgatagt gctgattggc ggttgtactg
    15541 gcagcggtaa aacgctgtta gtgcagcaac agccgaacgg tgttgatctg gaagggctgg
    15601 cgcgccatcg cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt
    15661 ttgaaaacct gctggctgcc gaaatgctga aaaccgacgc ccgtcaggat ttgcgcctgt
    15721 gggtgctgga agacgaaagc cggatgatcg gttcgaatca cctgccggaa tgcctgcgcg
    15781 agcgaatgac tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgagc
    15841 gcctgaacga agagtatttc ttgcgtatgc atcatgattt tacccacgct tatggcgacg
    15901 aacagggctg gcaggaatat tgcgaatacc tgcatcacgg actttcggcg attaagcgtc
    15961 ggctggggtt acagcgctat aacgaactcg ctgcccagct ggatacggcg ctgacaacgc
    16021 aactcaccac cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttaaagaat
    16081 attacgaccc aatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg
    16141 gtgagtgggc ggaagtggcg gaatgggtta aggcgcggta atgagggacg tatttgtatt
    16201 agtcgcgact tgatctgaca catggccttg aaaggttgag agttaccggt tttgatatga
    16261 gtgtcgaatc cttatccaaa acaagaggta actctcatgc ttcatactgc caatcccgtc
    16321 atcaaacaca aagccggttt gctcaatctg gctgaagaac tcagcaacgt atcaaaagcc
    16381 tgtaaaatca tgggcgtttc gcgtgatacg ttttatcgtt accgcgaact ggtcgctgaa
    16441 ggcggtgtgg atgcgcagat taatcgtagt cgccgcgctc ctaaccttaa gaaccgtacc
    16501 gatgaggcaa ctgaacaggc tgttgttgat tacgccgtcg ctttcccgac acatggtcag
    16561 caccgggcca gcaacgagct gcgtaaacag ggggttttta tctccgacag tggtgtccgt
    16621 tctgtctggt tactccataa ccttgagaat ctcaaaagac gatactgaac aaattttatc
    16681 aggtgacgtt ccgtaaaaag ttgtatggag ttctcgatgc agtcggcgcg actatcagta
    16741 cccccgctga accctggact atcgggccgc cgtactgggg catgtcaccc tggcgtgccg
    16801 ctggttttcc gctaatggtt tatttcctct ttctttcccg gcaagaattt attttaatcc
    16861 ggaaggatta tttattttca gatagggact cacattaaac acgtaacatt ttaattaatg
    16921 catggctgaa tatgtattac acaatgtcag atatcgtaaa agaccagtgc aattctatgt
    16981 aaactctctg actgttcatt ttatttgttg tttcagggtc ggaactcacc ctcaataaac
    17041 aactgaatac gagtagatat tcatcgtctg agctatatgg ctttacacaa tagccgacat
    17101 gtcttttctc agtcaggaat tttacttttc ctcactacga agttgacgac ttcgatatgg
    17161 gatagactct taattcaagc aattactctg cacaagcatc aggcaatcca gctccaatat
    17221 ttgttataaa ataatgcgta acattaattt caccaacaaa aaaaaggttt acaccatccc
    17281 cttgccctgg tatcttacca tatttacacc acactttttt tgtatctgga tcattctttg
    17341 atacgatttt cgccacttgt tccctggccc tcactgtcaa tgttttc
//
LOCUS       DAZFRJ010000087        17279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000087 DAZFRJ010000000
VERSION     DAZFRJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.087"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..785
                     /locus_tag="ACHKUN_002659"
     CDS             <1..785
                     /locus_tag="ACHKUN_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6660893.1"
                     /translation="TTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDHNCDG
                     RRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKYNKAH
                     AGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPN
                     CWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIY
                     SGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(856..1305)
                     /gene="rplI"
                     /locus_tag="ACHKUN_002660"
     CDS             complement(856..1305)
                     /gene="rplI"
                     /locus_tag="ACHKUN_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO6660894.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1347..1574)
                     /gene="rpsR"
                     /locus_tag="ACHKUN_002661"
     CDS             complement(1347..1574)
                     /gene="rpsR"
                     /locus_tag="ACHKUN_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO6660895.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1579..1893)
                     /gene="priB"
                     /locus_tag="ACHKUN_002662"
     CDS             complement(1579..1893)
                     /gene="priB"
                     /locus_tag="ACHKUN_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO6660896.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1900..2295)
                     /gene="rpsF"
                     /locus_tag="ACHKUN_002663"
     CDS             complement(1900..2295)
                     /gene="rpsF"
                     /locus_tag="ACHKUN_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO6660897.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2622..2897
                     /gene="yjfY"
                     /locus_tag="ACHKUN_002664"
     CDS             2622..2897
                     /gene="yjfY"
                     /locus_tag="ACHKUN_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO6660898.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3026..3712)
                     /gene="ulaF"
                     /locus_tag="ACHKUN_002665"
     CDS             complement(3026..3712)
                     /gene="ulaF"
                     /locus_tag="ACHKUN_002665"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO6660899.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3712..4566)
                     /gene="ulaE"
                     /locus_tag="ACHKUN_002666"
     CDS             complement(3712..4566)
                     /gene="ulaE"
                     /locus_tag="ACHKUN_002666"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO6660900.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4576..5226)
                     /gene="ulaD"
                     /locus_tag="ACHKUN_002667"
     CDS             complement(4576..5226)
                     /gene="ulaD"
                     /locus_tag="ACHKUN_002667"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6660901.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5240..5704)
                     /gene="ulaC"
                     /locus_tag="ACHKUN_002668"
     CDS             complement(5240..5704)
                     /gene="ulaC"
                     /locus_tag="ACHKUN_002668"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO6660902.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5714..6019)
                     /gene="ulaB"
                     /locus_tag="ACHKUN_002669"
     CDS             complement(5714..6019)
                     /gene="ulaB"
                     /locus_tag="ACHKUN_002669"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO6660903.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6035..7432)
                     /gene="ulaA"
                     /locus_tag="ACHKUN_002670"
     CDS             complement(6035..7432)
                     /gene="ulaA"
                     /locus_tag="ACHKUN_002670"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6660904.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7787..8851
                     /gene="ulaG"
                     /locus_tag="ACHKUN_002671"
     CDS             7787..8851
                     /gene="ulaG"
                     /locus_tag="ACHKUN_002671"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO6660905.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8959..9714
                     /gene="ulaR"
                     /locus_tag="ACHKUN_002672"
     CDS             8959..9714
                     /gene="ulaR"
                     /locus_tag="ACHKUN_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO6660906.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9711..10460)
                     /gene="yjfP"
                     /locus_tag="ACHKUN_002673"
     CDS             complement(9711..10460)
                     /gene="yjfP"
                     /locus_tag="ACHKUN_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6660907.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10642..10971
                     /gene="bsmA"
                     /locus_tag="ACHKUN_002674"
     CDS             10642..10971
                     /gene="bsmA"
                     /locus_tag="ACHKUN_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO6660908.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11120..11395
                     /gene="yjfN"
                     /locus_tag="ACHKUN_002675"
     CDS             11120..11395
                     /gene="yjfN"
                     /locus_tag="ACHKUN_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO6660909.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11512..13137)
                     /locus_tag="ACHKUN_002676"
     CDS             complement(11512..13137)
                     /locus_tag="ACHKUN_002676"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO6660910.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13221..14384)
                     /locus_tag="ACHKUN_002677"
     CDS             complement(13221..14384)
                     /locus_tag="ACHKUN_002677"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6660911.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14387..15025)
                     /locus_tag="ACHKUN_002678"
     CDS             complement(14387..15025)
                     /locus_tag="ACHKUN_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO6660912.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15035..15433)
                     /locus_tag="ACHKUN_002679"
     CDS             complement(15035..15433)
                     /locus_tag="ACHKUN_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO6660913.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15451..16110)
                     /locus_tag="ACHKUN_002680"
     CDS             complement(15451..16110)
                     /locus_tag="ACHKUN_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO6660914.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16161..16859)
                     /locus_tag="ACHKUN_002681"
     CDS             complement(16161..16859)
                     /locus_tag="ACHKUN_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO6660915.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16878..17279)
                     /locus_tag="ACHKUN_002682"
     CDS             complement(16878..17279)
                     /locus_tag="ACHKUN_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO6660916.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         4396 a         4614 c         4129 g         4140 t
ORIGIN      
        1 caacgactat ttattcattt cctgcatatt caccccattt ttcgtccacc gacaaaccag
       61 taaaattaaa atttcatcaa tatgcacgtg ctacattact gccaaatcgt ggaagagatc
      121 ataattgcga tggcagacgt actgttgaga tccacaaact agatctctct gactggcaag
      181 cattcaataa gttagcaacg aagtgtaatg cttacgatgg agtaacaatg aatggtgata
      241 attcttttgg atggaaccat aaagctacat tagataatat tcacgcccag aaatacaata
      301 aagctcacgc gggtgcgagg ttaaccgcag aactgaaata tttattgcaa gatgtcgaat
      361 ccttcgaacc caacagtaaa tacacaatac acgaagtggt tttgggacct ggttatggta
      421 ctccagacta taccggccaa acgataggat acgttgtaac ccttcctgcc caaatgccta
      481 attgctggtc atctgaacta ccaacaatcg atctttatat cgaccaactt cgcactgtta
      541 caggcgtttc taatgccctc ggattcatca ttgctgcatt gctaaacgcg tattctgatt
      601 taccccatga tttgaaaatt ggtctacgtt ccttatcaaa ttcagcagca atctactcgg
      661 gtttaggttt cgagcgagtg ccgcaggacc gagacataag gagtgatcgt atgcatctaa
      721 cgccagcaaa ccatcctgac ttatggacac aagagaatgg tgaatggatc tacttgcgaa
      781 actaatacct gacgaaaaat agaaagcaaa acgccgacca atggtcggcg tttttacgtc
      841 tcgttgaata acgaattatt cagctactac gtttacgatc actttcgcga atacttcgct
      901 gtgaacctgg aagctcactt cgtgttcgcc agtggtacgc agaacgccgt tcggcagacg
      961 aacttcgctc ttagccactt caacgccagc tgcagttaca gcgtcagcga tgtcgcgagt
     1021 accgatggaa ccgaacagtt taccttcgtc gccagcttta gacgcgatgg taacagtttc
     1081 cagtgcattg attttctcag cgcgagcatt agcagctgcc agaacttcag ccagtttagc
     1141 ttccagttca gcgcgacgtg cttcgaagaa ttcaatgttt ttcttggtag ctggaacagc
     1201 tttaccctgc ggtaccagga agttacgagc atagcccgct ttaacgttta cctgatcacc
     1261 caggctaccc aggtttgcta ctttatcaag cagaataact tgcattacct tatcctctca
     1321 aagtcgtatt aatggaccgt gaccgattac tgatggcgat cagtgtacgg cagcagggac
     1381 aggtagcgag cgcgtttgat agcgcgagcc agctgacgct ggtattttgc acgggtaccg
     1441 gtgatacggc ttgggacaat cttaccgctt tcggtgatgt agtttttcag cgtagcgata
     1501 tctttatagt cgatctcttg aacgccttcc gcggtgaaac ggcagaactt gcgacgacgg
     1561 aaataacgtg ccatatggct agtctccaga atctatcaat tcaatctgct cggcatgcaa
     1621 aaccattttg ctcagtccgt tctttgcctt gtggcatgaa atgaacccct gaacggttat
     1681 gcgactgccg accgttatac tgtgagtaat ggcctggttt tcgtgtccgc taacaataac
     1741 gggcatttga caccatgcct gccggtgaaa gccggcttcc tcctgcacag aacgatgctc
     1801 aagcacgaac tggcagtgag gaattcctga tggactgacc tttcgaaggg gagtcctgca
     1861 cacggtgccg gacaacacca gacggttggt catcagaaat tactcttcag aatccccagc
     1921 ttcagcatca tcagcggttt cgtttgcgaa atcatcgcga cgctcacggc gctcgtcttt
     1981 cgctttaacc atcggagatg cttcggtaac agcgtgcttg gtacgcataa ccatgctgcg
     2041 gataacggca tcgttgaagc ggaaggtagt ttccagctca tcgatcactt cctgcggagc
     2101 ttcaacgttc atcagaacgt agtgtgcttt gtgcagtttg ttgatcgggt aagccagctg
     2161 acggcggccc cagtcttcca gacggtggat cttgccttct gcaccagtga tggcagcagt
     2221 gtagcgctcg atcatgcccg gaacctgttc gctctggtca ggatggacca taaaaacgat
     2281 ttcgtaatga cgcatcgaat tgctccttac ggattattca gcctcctgtc tgggtcagcc
     2341 gaatcccggg gaggcaagga acgtgttaaa ggtcggctga aaaatgacgc gttatagtac
     2401 ttgtatgccc cgctaaactc aaggccagcc gacaaataaa tctcatcttt cgctcaaaag
     2461 aactaactcc ctgatttgaa gttcatcaca ttccgtcaca gtgttttttc gaacaactgc
     2521 atcagaaatg gtcaccatca acttctttat cattgattaa aattaaatca gaagatcatc
     2581 acagaggaga gcacacaaag tcagccgttg aggaggtatc tatgttcagt cgtgttttag
     2641 cccttctggc tgtgcttttg ctaagtgcaa atacatgggc agccattgaa atcaataacc
     2701 accaggccag aaatatggac gatgtgcaaa gcttaggcgt gatttatatc aatcataatt
     2761 tcgccactga aagtgaagca cgtcaggcat taaatgaaga aacagatgcg cagggcgcaa
     2821 cgtactacca cgtaattctg atgcgggaac cggggagtaa cggcaatatg cacgccagcg
     2881 cggatattta tcgctagcac caggtataac caacgaaaca ttgccatagt ttgctttgcc
     2941 cccttcgcag ggggcttttt ttgaaaaacg gctgcggaat tagaccagtt atctcccgaa
     3001 gaaggaaatt tccgcagcgc gtgttttact tctgcccgta ataagcgtta ggaccatgct
     3061 tacgcatgaa gtgtttattc atcaggaagc tgtcgatgtg attgagttgt ggattaatac
     3121 tgcgcgcaat ccacgccatt ttcgccactt cttccatcac taccgcgtta tgcaccgcgt
     3181 cgtgggcatc tttcccccag gcgaacggtc cgtgctgata caccacaatt cccggcgtat
     3241 gcagcggctc ggcgttgccc agcgtttcga taatcacttt gccagtgttc agttcatact
     3301 caccctgcac ctcttcttcg cttaatccac gcgtacacgg gatgtcacca aagaagtagt
     3361 cagcgtgcgt ggtgcctaac gccgggatcg ccagccccgc ctgcgcccat gcggtagcat
     3421 gagtggagtg ggtatggaca atgccgccaa gcgacggata acgacggtag agttcaagat
     3481 gcgtcgcggt gtcggaagat gggcgatact ccccttccac caccttaccg ctcatatcga
     3541 ccaccaccat atcgtctgct ttcatggttt cgtaggcgac gccgctgggc ttgatcacca
     3601 ccagcccgcg ttcgcggtcg atagcgctga cgttgcccca ggtaaaggtc accagcccgt
     3661 agcgcggcag atccatgttg gcttcaaata cctgctgttt tagcttttgc attatgccgc
     3721 ctccaccatg cccgctttcg ccatgcgcgc tttcacccaa tcacgcgctt tcgccacttc
     3781 tgccgccggg tcttccgccg tttcgctcca catctcaatc aggtacggcc cgcaatagcc
     3841 actctgtttg agcgtttcga aacaacgttc gaaatccact acaccttcgc caaacggcac
     3901 gtttttgaag acgccaggtt tggtgtcttt cacatgtacc gcgacgatat gcccgattcc
     3961 ggcctgcaac tccatctgca catcgttgtc ccacgccgac aggttgccga tatccgggta
     4021 gagctggaac cacgggttgt tgagatagtg cgcgtaaccc agcgccttgc tgatggagtt
     4081 catcaacgga taatccatga tctccatcgc cagcgtcacc tgcgcgcggc tcgccatctc
     4141 aacgctctct ttcaggccgt cacggaaacg acgacgcgtt tcgttattgg cttcctgata
     4201 gtaaacgtca tagcccgcca gctggatcac gcgaatgccg acgtcctggg cgaactggat
     4261 agctttacgc ataatctcca gcccctgcgc ccgcaccgcg tcatcttcac tgcccagcgg
     4321 gaaacgacga tgagcagaaa ggcacatgga cggcacgcgc acgccggttt caacaatcgc
     4381 attcaccagc gccagacgct gctcgcggct ccagtcgagg cgcgacaggc gctcgtccgt
     4441 ttcatctacc gacatctcga caaaatcgaa gcccaacgtt tttgccagtt gcaggcgttc
     4501 cagccagcac tccccggcgg ggagcgcttt ttcatagatg ccaagcggga tttgtttgga
     4561 caacatatcc gctccttagc cccacagttc ggcgatggaa cgtttgaact gacgtgcagc
     4621 ttcaaccgga gaagcggcat cacggatgct gcgaccagcg ataaacacat gaatcggaat
     4681 acctttgaac agcggcagat cttccagcgc caggccaccg gtgacggtga ctttgaagcc
     4741 catatcggaa agacgtttga tcgcggtaat gtccgcttcg ccccacgcta cgcctgcggc
     4801 ctgcgcgtca cggctgcggt gataaaccac ctgctgaatg cctgcgtcac gccactgctg
     4861 cgcctgttcc caggtccagt agccggtcaa ttcgatctgc acgtcgccgt taaactcttt
     4921 tgctacgtcc agcgcgcctt tggcggtgtt gatatccgca cagcaaatta ccgtcaccca
     4981 gtcagcgttg gcttcgaagc acatacgcga aaggattttg cccgcatcag caattttagc
     5041 gtctgccagt acgattttgt gcgggtagag cgctttcagg tcacgaaccg cacgcacgcc
     5101 ttcgcccacg cacagaatgg tacccacttc gataatgtcg acttcttcgg caatcaggcg
     5161 agtggtttcg taggcgctat ccatagtctg gttgtccagc gcgacttgca acatcggtaa
     5221 tgacatcttc aattccttct taagctgccg cgttggtgcg gtcaatgaga tccagtactt
     5281 cctgctcggt acggcaggca cgtaaacggt cgaaattttc ttcatcttca aacaggttga
     5341 cgatctgcat gatgcccact tcctggtgcg tgttggcatc gaccgccgcc atggtgatga
     5401 ggatatccac cgggtcgtta tcttcgtggt tgaactccag cggctttttc agcgtcacca
     5461 gcgcaaaacc ggttttctta acgccctctt ccgggcgacc gtgcggcatt gccaggcctg
     5521 gtgcgagcac aaagtacgga ccgtgctgtt caaccgcatc cagaatcgcc tggtagtaac
     5581 gcggctcgac aacatccgcc gccaccagca ggtcaacgcc gatttttact gcgtcctgcc
     5641 atgtctcagc ttcagcctgc aggcggatgg atttattctc cgccagcgaa tcacgtaatt
     5701 tcatggcgcg tccttacttc acatcctgcg ggaaatgcgc tttgatcact tccagcagtt
     5761 tcgggccaaa gtcggcagga gagagcatgt tgcgcacgcc aaccacgtat ttgttgccgg
     5821 tcacggtgat ttcgcccgca atgtgcgtag aagcgatgat gatatccgcg ccgctcaact
     5881 cgcttttgta ttcgccaacc gcgcagctat ttaccgtgtg gtcaatgttt gattgggtta
     5941 aaaactggtc cactttcatc ttcatgatca tggaactgcc ttgtccgttg ccacacacag
     6001 ccagaatacg tacggtcata atcaaaactc cttattaagc agactgttct gccagttgtt
     6061 tttctgcatc ttcttctgcg cgcagtgcgc ggccagcgaa gaacatataa gccagtgcaa
     6121 ttacaatgat gacggccata aaggcgatac cgatggagaa gaagccctgc atcatcggcg
     6181 gtgccagaat cgaccagtcc gccatgccca tccaggcgct cataccggtg agtttcaccg
     6241 cccagacgca gccaaagatt tcaatcatcc ccatcaccag acatatcttc agcgccgcac
     6301 gccagccgcc gaagtggtta gcgaacacgc cgatggtggc gttggagaag aacatcggga
     6361 taaagccggg aataatcagg atcgaggaac cgcaggcgac caggatgcca accgcaatca
     6421 gctgaccgat ggtgccccac ataaagcccc aaaccacggc gttcggcgcg aagctataga
     6481 tagccgcaca gtcaatcgcc agaaccgcac cagggatcag gcgctgggag ataccgttaa
     6541 atgcttcaga gagttccgcc acaaacatgc gcacgccctg agtgatgatg aagatcgcca
     6601 ccgcgaagga gaaaccagtt tgcaggatgt acaccgtcca gttcactttg cctgccatcg
     6661 cctgcacggt gtcgataccg aaggagagca gaatggcacc aaagaagatg gtcatcacaa
     6721 tcgccgtgga gacgatgttg tcgtggaaaa tattcagcca gcctggcagt ttgaggtctt
     6781 caacgctttc ctcttttttg ccgaggaacg gcgcaacttt ataggcaatc catgatgcaa
     6841 actgttgctg gtgaccgatg gagaaaccgc agccgtccgt cacttcctga gtcggcttgt
     6901 acatcatgtt ggaggtaatg ccccagtaga gcgaaaccag aatcgccgtg cagataatgg
     6961 ttgtccacat ggagtagccg aaaataaaca acgtaacggc aatcagcccc gcctgctgga
     7021 acatgatata accagtcagc atgatggtac gaataccggt aatgcgacgc agcagcacgt
     7081 aacagatgtt cagcgccagc gctaacaaca ccgcgtaacc tacccagcta taggcatcgc
     7141 ccatgcggtc gatggttgcc atcatcgacg cgtaagtatc ggaaattgcg ccgttaatgc
     7201 cgtagacttc ggacattttc gccaccaccg gtttgaaggt gctggtgagg atgccggacc
     7261 ctgcctgcaa caacatgaaa ccaattatgg ttttaatcgt gcctttaata ataacgctga
     7321 cacttttgcg cagtaggatg tagcccagac aggtcacaat acccagcaac aacggggcat
     7381 tggtcatgac ctggttaaaa aacacggtaa agatgttgta gaggatctcc ataacgatct
     7441 cctgaagaag aggtaaccgg gtattccgca cacccggaat gttgtgcact cactctaatt
     7501 ttcaaaagta atcacaacaa gattatttat gattaaatgt gacgcgaccc gcaaataatt
     7561 ccacacggat agagtgggat tcacacaacg aaacaattat ttatttaaat tcatttagtt
     7621 aatttaacta cacattccat taaatctttc cacactcaaa atcaacaaca acttccctgg
     7681 caaaaagtca aaaaatggtc attgccttcc tgaaaaaata gtgtcaggat taatcaaaac
     7741 caatcacata ttgattcgaa ttgaactatc atgaaggtaa atggcgatga gtaaagtgaa
     7801 aagtatcacc cgagaatcct ggatcctgag cactttcccg gagtggggta gctggctgaa
     7861 tgaagaaatt gaacaagagc aggtcgcccc tggcacattt gcgatgtggt ggctaggctg
     7921 caccgggatc tggttgaaat cagaaggtgg caccaacgtt tgcgttgatt tctggtgcgg
     7981 cactggcaaa caaagtcacg gtaacccgtt aatgaaacag ggtcaccaga tgcagcgcat
     8041 ggctggcgtg aaaaaactgc agccaaacct acgcaccacc ccgtttgttc ttgatccgtt
     8101 tgcgattcgc cagatcgacg cggtactggc gactcacgat cacaacgatc atatcgacgt
     8161 taacgtcgct gctgccgtga tgcagaactg tgctgatgac gtaccattca ttggaccgaa
     8221 aacctgcgtt gatttgtgga ttggctgggg cgtaccgaaa gagcgttgca tcgtggtcaa
     8281 accgggcgat gttgtaaaag taaaagacat tgaaattcat gcgcttgacg ctttcgaccg
     8341 tactgcactg atcaccctgc ctgccgatca aaaagcggct ggcgtactgc cggacggcat
     8401 ggacgatcgc gcggtgaact atctgttcaa aacgcctggc ggctccctgt atcacagcgg
     8461 cgactcccac tactctaact attatgcgaa gcacggtaac gaacatcaga tcgatgtggc
     8521 gttaggatcg tatggtgaga acccgcgcgg tattaccgac aaaatgacca gcgccgatat
     8581 gctgcgtatg ggtgaagccc tgaatgcgaa agtggttatc ccgttccacc acgacatctg
     8641 gtcaaacttc caggccgatc cgcaagagat ccgcgtgctg tgggagatga aaaaagatcg
     8701 tctgaagtat ggcttcaagc cgtttatctg gcaggttggc ggcaaattta cctggccgct
     8761 ggataaagac aacttcgagt accactatcc gcgcggtttc gatgattgct tcactattga
     8821 accggatctg ccgttcaagt cattcctgta atctgtcgtt tataccggat agcgtggaga
     8881 agttcgcgct atccggcgta gtatttcctg gctatttcaa atatcatcta aaaaaatcaa
     8941 attttatcgg aatagctcat gactgaagca caaagacatc aaatcctcct ggaaatgctc
     9001 gcacaattgg gctttgtgac cgttgagaaa gtcgttgagc gtctgggaat ttcgcctgcc
     9061 actgcgcgac gcgatatcaa taaacttgac gaaagcggca aactgaaaaa agtgcgcaat
     9121 ggcgcagaag ctattaccca gcagcgcccg cgctggacgc cgatgaatct gcatcaggcg
     9181 cagaatcacg atgaaaaagt acgtatcgct aaagcagcct cgcagctggt taatccgggc
     9241 gaaagcgtag tcatcaactg cggctccacc gcgtttctgc ttgggcggga aatgtgtggc
     9301 aagccagtgc aaatcatcac taattatcta ccgctggcaa attacctgat cgatcaagaa
     9361 catgacagcg tgatcattat gggtggacag tacaacaaaa gtcagtccat cactttaagc
     9421 ccgcagggca gcgaaaacag tctctatgcc gggcactgga tgtttaccag cggaaaaggg
     9481 ctgaccgcag aagggttgta taaaaccgat atgctgacag caatggcaga gcagaagatg
     9541 ctgagcgtgg tagggaaact ggtggtactg gttgatagca gtaagattgg cgaacgcgcg
     9601 ggaatgcttt ttagccgtgc cgatcaaatc gatatgctga tcaccggcaa aaatgctaac
     9661 ccggaaatcc tgcaacaact ggaagcgcaa ggggtcagca ttctgcgtgt ttaaagatgc
     9721 tggcggaaaa atgtcacggc agcatctaac gcctcaggcg taatgcggtg acgcacgcct
     9781 ggctgccatg aacaggttag cagtttatcc cgtcccgtct cgcttaaggc ctgctgcaaa
     9841 cgtagtgatt cgtcggcagg cacaacgtca tcgtcgaggc catgccacag cagtagaggt
     9901 ctgtccccaa gttgttccag gtggtttgtc gcttcccact ctgccagcgg cgcgataatg
     9961 ttattgaatt cattctgctg tgctgccgtt tcaggtatca gcggtggaaa cagtgaacgg
    10021 gcgagcgatg taaaatagcc cgatcccatc atgctggcgg tacatttcac cgtggggtgg
    10081 cgagtggtaa ttcccagtgc cgtcatcgcg cccatcgacg cgccaccgac tgccagacgg
    10141 tcatcaagca gccagttttc ttcggctatt gccgcacgta aagtagtgaa ttcctgcata
    10201 ctttgtagca agatttgcca gaattgattt aaccgccgcg cttcgtcgct actaaaacgg
    10261 ctaccgtgat cgggcgcatc cggcatgatc acccgcaaac cagcctgcgc cagcgcaacg
    10321 gcaaaatagc tatacaccag gctggatgaa gtaaagccgt gataaaaaat tacgcacggt
    10381 aacggggtat ctttttgccc gacaggataa gcatgaagaa cgggaatatc tgccagctcg
    10441 cgtgattcta tttcaatcat ctgtctctcc tttttgtctg gtatgacatg aagaaatcct
    10501 tagcacaagt ttctgctggc ggtattagtt cataatgttg agatgtgggt tacgctttcg
    10561 tcacgttttc attcgaaaat cgcgtaccag gtaacaattc gggaacattc ccccaaagtt
    10621 aaattaataa ggcactacac tatggttagc aggaaacgta atagcgttat ttaccggttt
    10681 gccagtttat tattggtgtt gatgttaagt gcctgtagcg ctctgcaagg tacgccacag
    10741 ccagcaccgc cagtaacgaa ccatccgcaa gagattcgcc gcgaccagac gcaagggtta
    10801 cagcgaatag gtagcgtaag cacgatggtt cggggttctc cggatgacgc attagcagaa
    10861 atcagagcaa aagctgtcgc tgcaaaagct gattattacg ttgtcgtcat ggtggacgaa
    10921 accatcgtga caggacagtg gtattcacaa gccattttgt accgtaaata aatcagacaa
    10981 cttacatagt cagctttaca ttgctttgcg tccctatgcg tttacctgtg cacaataaat
    11041 taccggctgg aagcctgggc gtttattacc gcgatggaat gccctgcaac atgtggggaa
    11101 acgaaaaatg gagctgacga tgaaacaatt acttgcctca ccctcgctgc aattagtgac
    11161 ttatcctgcg agcgccacgg cacagtctgc agaattcgcc agtgctgact gcgtgacggg
    11221 cctgaatgaa ataggtcaga tctcagttag caatatttca ggggatccgc aggacgtgga
    11281 acgtatcgtg gcgttaaagg ccgatgaaca aggtgcgtca tggtaccgca ttattaccat
    11341 gtacgaagac cagcagcctg ataactggcg cgtacaggcg atactgtatg catgagtatt
    11401 atatacagca aatgaacaat gccggatgcg gcgcgagcgc cttgtccggc ctacaatgtt
    11461 cgcatatttc attgagttaa tcgaaaccag cgtcgcatca gtcgtatacg cttacacaca
    11521 cactcccccc gtcgcccgca gcaataaatc attctggatc tgctctgaca accgtacgcc
    11581 gccgcgcgta tctaacatca cctgacacca cgcctgcgcc attggcggag aggcatattt
    11641 caacatttgc gcaccgcagc ccagcaggaa tagctgatga gtaatctctc gccccagttc
    11701 ttcagctggc ttgcgcagct gctgctgtaa acgacgaacc gcgcgatcaa aatagcgatc
    11761 ctgccctttc acttccacaa acgcttccga caataagtcg tatacgcccg cttgcttatt
    11821 gagaacgcgc agcacatcca ggcacataat attgccggaa ccttcccaaa tactgtttac
    11881 cggcatctcc cggtaaagcc gcggcagctc gctctcctcg caataaccaa tgccgcccag
    11941 cacctccatc gcttcggcca caaacggcat acctcttttg cagatcacaa attttgccgc
    12001 aggcgtaaat aaacgcgccc acagggcttc tttggcatcg gcacgccggt cccacgctcg
    12061 cgcaagacga aacagcaacg ccgtttgccc ttcaagctga agtgccatgc gacttaagac
    12121 atgacgcata aggggctgtt ggatcaatgg attaccaaaa acatggcgtt gatgtgcatg
    12181 ataaatcgcc agcgaaaatg cacggcgcat catggcatgg ctacccaggg cgcaatcaaa
    12241 acgcgtcatc ccacccattt tcaggatcag acgaattcct tccccttcct gccccaacaa
    12301 ccagccaatg gcatcctgaa actccacttc gcagctggcg ttagagcgat tacccagctt
    12361 atctttcagc cgctcgaggc gaatcgcgtt gcgttgcccg tcaggcaaaa agcgcggcac
    12421 aaaaaagcag gacaaaccgc ccgtagtctg cgccagcacc agatgcgcat cgctttgcgg
    12481 caccgagaaa aaccatttat gccccaccag ccgataagag ccatcttcca ggcgctctgc
    12541 gcgggtggtg ttgctcatga catcggaacc gccctgcttt tccgtcattc ccatgccaat
    12601 caacaaaccg cgtttttgcc cacctggcaa taagtgagaa tcgtagcgat cgctcagcag
    12661 cggcgtggtc cagtcctgaa acggcgtggg taacatctgt aacaacaatg gcgtggcggc
    12721 aaaggtcatc gttattggac ataacgaccc agcctcaacc tgcgcatgta acataaaacg
    12781 cgccgcgcgc gccacaaatg cgccggagcg agcgtcttct tcccaggcaa gattgtgcac
    12841 ccgattggta cataacgcct gcatcagcag gtgccaggcg gggtgaaaac gcacatcgtc
    12901 cagacggcgt ccttgcgcat cgtagcgcaa taattcaggc ggattcacat tcgccagccg
    12961 ccccagttca agagattcag ccgttcctaa ctgctgacca atactggcta gaaaatcgct
    13021 atcccagcca gcaccttcac gcgttaccgc ttcacagagc gcgccatcag acaggtataa
    13081 gttgctgtta tttaatggta taggttgatt aaaaacggtg tgagtttgcc agtgcactgt
    13141 gtctccctcc atcaatggca gtcaccctta gtatggtcac tgccatggat tcctgacaga
    13201 aagagattgc taaaacattc ttatccagca atgtaatgcg gaacgaaacg tgaggtgtct
    13261 ttggtgatca gcgtgttatc ttcacggatc cccattccgc acgcttcatc atcgacaatc
    13321 cagctaccga tgagtgtgta gctatcgcca aatcgcggca gaggttgaaa cgcctggtag
    13381 atcatcggtt catcggcgta atcaccatca gcgtggtcaa cgacgttatt ctgaccgtcg
    13441 aaaatggtga cgtttccgcc ttcgcgcgag tagatcggtt tacgcacata gctttcgcca
    13501 gcggcaatct gcggtttttc gccctcaaac caggatgcaa gaaggttagg atgaccaggg
    13561 aagaagcgcc aaagcagcgg cattagccct ttattactca agatactttt ccataacggc
    13621 tccacccatt gctcgcgacg cttgcgcagc agcggaccgt tatcgtcacg catcatccac
    13681 tccagcggat acagcttaaa tgcacgctgg atgacattat catcaagatc ggtcagtaca
    13741 ccgccgacgc ccaaaccgag atcttcaatg tagataaacc gcgactcctg ccctgcctgc
    13801 tgggcgcagt cctgcaagta cagcacggta gtcctgtctt catcggtgtc ctgacagcag
    13861 cagaaataaa acggctcccg gctataaagc tcgctaaagc gtgaaatcag gcgttcctga
    13921 atggcattgt actgatcggc atcacgcgga ataatgccgc tgcgccgggc atcctccagc
    13981 cacaaccact ggaaataagc cgactcatac aacgaggtcg gcgtatcggc gttgtactcc
    14041 agcagcttca ccggcgcatt gccacaccag gcaaaatcca tccggccata cagcgaagga
    14101 tcgcgtgcgc gccagctttc ggcgatcaca tcccagtaca acggcgggat cgccagttgc
    14161 gtcaggatct cttcgtcttt aaccgcgcga tcaaccacct caaggcacat ctgatgcagt
    14221 tccgcagtcg gtttttcgat ctgctcttca atctggcgca gagtaaaacg gtaagcccga
    14281 ctctcgtccc aatagatttc attgtcgatg atatgaaagt cgaaaccgtt gttagcggcg
    14341 atctggtcca gatcccgtcg cacaggaacg ttgtgtctca gcatgattag cctccccaat
    14401 gcccacgggc gctggaagaa cgaccatagc cgccgcgcga aacggttgac gcttttttgg
    14461 tggtaaagcc gcctgaagag taagactctt ttttgccaga accggagcgc caggagtaat
    14521 caccggaagt gttgcgccag acggggcggg aagcaaatga ggatccgccg ctgttataga
    14581 cgaacggctc atcgcgatct ttacgaataa cccggcttaa caaaaatcca gaaacgaccg
    14641 ggatccaact ctgttcaaca ttgtcgtagt agcaattttc gtacttagac tgacaattct
    14701 gctgagtcat attcttcgga acatcggcat aaaatgccgc tttggcgttg ttccagccac
    14761 gcgcgcaaat gtcggcatta ttaccgtcat caatacaatc ctgcactgtc gaataaaacg
    14821 ttccgtcgcc gtcgttatcg acatcgccac tatcgccaca acctttcaat acaaaaaaag
    14881 cggcaccgcc cattagagcc aacgttaagt atttttgtgc gtagcgattg cgacgcggtt
    14941 caaaaggatt attcggtcta ccaatgcgac tgatcgctcc gtgaccaatt ttactgttat
    15001 ttcttgattt gcgttttctg gccatgatcg tccattacca tgtcatacaa gctgcgttaa
    15061 aaataccgcc agccagcgcg gcggttccca taaacattcc tgctgcggta ttgtgattaa
    15121 taattttttc gcttaatgcg ggcatataaa gtctgacacc agcaaaaacg agtaactgaa
    15181 tcaccagtgc aatcccgccc caggcgaaat agtctggaat actcaccgca ttgattgccg
    15241 cactggataa tgggataacg taacctaaca atgtaccact gaacgccagt gaagccgcgg
    15301 tattattgtt tttgattaac tgccattcgt tgtgcggtgt aattttagaa tagataaaaa
    15361 gaaaaataat caccatagcc acgccaataa aaaaataggc gctaaaggca agaagtgaat
    15421 ccagtatatg cattgtaaac gtccttgtaa ttaaccaata atatgaagtg atgtcagcgg
    15481 gatatcgaca cccaacgcgc gcgaaaataa ccactctggc tcgccgagat cgttaaaaga
    15541 ttcctcacca tttaacagca aatattcgta agtatcttcg gttacttgcc gctggtagcc
    15601 catggtaaaa ttatggacct cccatttggc atggttttga ttttctactt tttccagcat
    15661 gtagacgggt tcgatatttc ccggttcttc gctgttaaaa aatcgctgcc agcgtttttc
    15721 ctgccagttt aagatcattg cccccatcac tttggcgttg atcgcctcgc gccagtgact
    15781 ctctttactg ataccgtagc tctcttcata gacaaagagc ttgatatcat caatgtcatc
    15841 aatatcttcg ccgccggtgg tattgatttg cagaaattca tcgcctgaag tgtagtagcg
    15901 gaaaatctga ctaccgccgc ccagatcgat gtggctgaca gcggctaccg taaattcctc
    15961 acccggcagc gcaatcagca attcatcttc cagcaaacga aacgctaacg tgtcgagcgt
    16021 aaagccacta ttgagatgaa gtcccagcgg accgcgagcg atagcaggct tattatcctt
    16081 gccaaacaga cgctggaaaa aaccagacat attttatcct taaaatagtt gccagccttt
    16141 tagcgaggct ggcaaaaaaa ttactcgccc tgttggcgtt gcagtcttgc taatacatcc
    16201 tgggcgctac ttttattgct accgccaatt ccggcttccg ccagcttttc atcaagatcg
    16261 cgaccgtctg cgactttttc caactgtgca gcagcatcca ggcgagcctg acgttcggcc
    16321 tgacgcgttt gcaggcgttt taaggattct gccgctgtcg aaacgctgga agatgcgcca
    16381 acggtagagg ttgttacagc ctgttgtgca cgctgcatgg cttcagtggc tttaacgact
    16441 tccatttgct gctcaaactg agcgatacgc tgcgctgtcg ctgtaactgc tttttccacg
    16501 ccatcacggg agacttccag attcgacaac acttgctctt cagcggtaat gagattctca
    16561 aggcgcgcga tttcttcagc aacttcgtta atcaacgacg gattaacgtt cttgcttaac
    16621 gcttccagcg cacgggcttc cagactggct ttgcgctcac gtaaatcttt cagcttatcg
    16681 tgacttaatt tcacccgcgc cagcagatca acgcgagatt ttccggcttt atcgagttca
    16741 gctttagcgt cacgaatatg ctgttccagc atgcgcacgc cctgggtttc ttcaatggat
    16801 tcttccgcct gggagataaa cgatttcccc agcgtaaata aactttttaa aattcccata
    16861 aatacccgtc cttgtaatta gtgtgaatat tcttctgtaa tttcagccag atccaatgcg
    16921 ttatctacca gcgaggttat ctcaagcagg atatcttcaa gagaagattt aagcgataac
    16981 gccccgaaaa caatgtaata ctcttcctgt tgcacgctgg agattcctac cgatgacaac
    17041 ggcatcatct tctgatttct taataagaag gtattaaatt catctggatt gctgatacta
    17101 cttaccggac aaataaaggt ttcgataatc atttggcggg aagtaaaaag aatattgatt
    17161 tgcaaatcgc cataatcatt cattttaata attaatgcgc tctcgctatt tgtcacatca
    17221 atattttgtt caggtacagt ttgcagcgcc gtcgccagcg ccaacggatt ccatgtcat
//
LOCUS       DAZFRJ010000088        17220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000088 DAZFRJ010000000
VERSION     DAZFRJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.088"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..327)
                     /locus_tag="ACHKUN_002683"
     CDS             complement(22..327)
                     /locus_tag="ACHKUN_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6660917.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            524..724
                     /locus_tag="ACHKUN_002684"
     CDS             524..724
                     /locus_tag="ACHKUN_002684"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6660918.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPAGCVMVFAARIRQGVGL"
     gene            721..951
                     /locus_tag="ACHKUN_002685"
     CDS             721..951
                     /locus_tag="ACHKUN_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660919.1"
                     /translation="MSQEITLQQAVERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLIDEQERRTLISNEKKCGGVHVQ"
     gene            941..1390
                     /locus_tag="ACHKUN_002686"
     CDS             941..1390
                     /locus_tag="ACHKUN_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660920.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQKEEREEYTLFAL
                     ECIRYTATRSLNDAKNILVADCENGGGVMRDDRFNSLKQEFSGVPDDAADALSSMPEL
                     IRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1383..1616
                     /locus_tag="ACHKUN_002687"
     CDS             1383..1616
                     /locus_tag="ACHKUN_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660921.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDILHDRYKEQHTGE"
     gene            1622..1921
                     /locus_tag="ACHKUN_002688"
     CDS             1622..1921
                     /locus_tag="ACHKUN_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6660922.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVTIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            1918..3318
                     /locus_tag="ACHKUN_002689"
     CDS             1918..3318
                     /locus_tag="ACHKUN_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO6660923.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            3519..3770
                     /locus_tag="ACHKUN_002690"
     CDS             3519..3770
                     /locus_tag="ACHKUN_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660924.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            3767..4072
                     /locus_tag="ACHKUN_002691"
     CDS             3767..4072
                     /locus_tag="ACHKUN_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660925.1"
                     /translation="MSEPKFGEKLYKHNGLITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            4082..4492
                     /locus_tag="ACHKUN_002692"
     CDS             4082..4492
                     /locus_tag="ACHKUN_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6660926.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGDDDPYGDDIPF"
     gene            4503..4775
                     /locus_tag="ACHKUN_002693"
     CDS             4503..4775
                     /locus_tag="ACHKUN_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6660927.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPDNYGDLKKAVDRTYAEMGMDGAGDEIWRNYQDS"
     gene            4732..4860
                     /locus_tag="ACHKUN_002694"
     CDS             4732..4860
                     /locus_tag="ACHKUN_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6660928.1"
                     /translation="MVLVMKYGEITRTANHTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            4901..5125
                     /locus_tag="ACHKUN_002695"
     CDS             4901..5125
                     /locus_tag="ACHKUN_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660929.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            5417..6574
                     /locus_tag="ACHKUN_002696"
     CDS             5417..6574
                     /locus_tag="ACHKUN_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6660930.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGATFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            6614..7186
                     /locus_tag="ACHKUN_002697"
     CDS             6614..7186
                     /locus_tag="ACHKUN_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6660931.1"
                     /translation="MGEKIMKSMEIRSSEITTSGSNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            7188..8399
                     /locus_tag="ACHKUN_002698"
     CDS             7188..8399
                     /locus_tag="ACHKUN_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6660932.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNMEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            8396..8734
                     /locus_tag="ACHKUN_002699"
     CDS             8396..8734
                     /locus_tag="ACHKUN_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6660933.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWLDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            8731..9024
                     /locus_tag="ACHKUN_002700"
     CDS             8731..9024
                     /locus_tag="ACHKUN_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6660934.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            9024..9464
                     /locus_tag="ACHKUN_002701"
     CDS             9024..9464
                     /locus_tag="ACHKUN_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO6660935.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREDEERAANRNNWMYDADE"
     gene            9448..9630
                     /locus_tag="ACHKUN_002702"
     CDS             9448..9630
                     /locus_tag="ACHKUN_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660936.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            9753..10109
                     /locus_tag="ACHKUN_002703"
     CDS             9753..10109
                     /locus_tag="ACHKUN_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO6660937.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            10093..11754
                     /locus_tag="ACHKUN_002704"
     CDS             10093..11754
                     /locus_tag="ACHKUN_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6660938.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            11768..12049
                     /locus_tag="ACHKUN_002705"
     CDS             11768..12049
                     /locus_tag="ACHKUN_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660939.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRALELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(12808..12951)
                     /locus_tag="ACHKUN_002706"
                     /pseudo
     CDS             complement(12808..12951)
                     /locus_tag="ACHKUN_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            13363..13728
                     /gene="mdtJ"
                     /locus_tag="ACHKUN_002707"
     CDS             13363..13728
                     /gene="mdtJ"
                     /locus_tag="ACHKUN_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO6660940.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            13715..14044
                     /gene="mdtI"
                     /locus_tag="ACHKUN_002708"
     CDS             13715..14044
                     /gene="mdtI"
                     /locus_tag="ACHKUN_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO6660941.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(14083..14904)
                     /gene="ydgD"
                     /locus_tag="ACHKUN_002709"
     CDS             complement(14083..14904)
                     /gene="ydgD"
                     /locus_tag="ACHKUN_002709"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO6660942.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(15004..15087)
                     /locus_tag="ACHKUN_002710"
     CDS             complement(15004..15087)
                     /locus_tag="ACHKUN_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660943.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(15180..15488)
                     /gene="asr"
                     /locus_tag="ACHKUN_002711"
     CDS             complement(15180..15488)
                     /gene="asr"
                     /locus_tag="ACHKUN_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO6660944.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            15759..15863
                     /locus_tag="ACHKUN_002712"
     CDS             15759..15863
                     /locus_tag="ACHKUN_002712"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO6660945.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(15912..17165)
                     /gene="ynfM"
                     /locus_tag="ACHKUN_002713"
     CDS             complement(15912..17165)
                     /gene="ynfM"
                     /locus_tag="ACHKUN_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6660946.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
BASE COUNT         4629 a         4081 c         4667 g         3843 t
ORIGIN      
        1 acaccacggc gctgatggtg attactctgg ttctttagtc ttgcggcgct ggagttcttc
       61 acgtgcgaca gtgacgagct gcccgatctc ctcggcggct ttgattccga gtttttccac
      121 ctgcgccagt gcattgagcg aagaaatcag gaggttttct ccgcttcctt ctgcctggcg
      181 gcgggcgatt tcaccgcgca tggcggttac tatgaatccg gcgttgcttt caccgtccag
      241 ttttacggat tccatgcctt caataacatc atgcgggatc cgagctgtca gtgattgtga
      301 ttttgcgttt tttgaacctg tagccatctg taatcctctc aatgaaagtg taagacaata
      361 tacacataaa aagtcttaca taaaagcatt gacatgtaag ccacctataa ataaagttac
      421 ttacaccttg ttaatgcaag gtgcagaaac gacgaaaccc cgcactgtag gagcagtaac
      481 ggggcttcta accaccaacg ataacgagag tatcgaggta gctatgagaa atcataccac
      541 acacccgcaa gggcgggact cgcacaacct gaataaatac atttggcgtt ttatcgccct
      601 gagcactgca caaccgcgcg tgattaccat tgaggccacc agcgaacagg aagcacgcct
      661 gcaatccccg gctggctgcg tgatggtatt cgctgcccgt attcgtcagg gggtaggctt
      721 atgagccagg aaatcacact acaacaggca gtagaacgcg cccaccaaat cgaagttatt
      781 tgcgcactgg cagaggatta cccaggcatg atgaccgaca gcgaatcagg ggcaatcatc
      841 ggcttactta aacgccttag cggtgaggtc tgcgtattcc tgatcgatga acaggaaaga
      901 agaacgctta tttctaacga aaaaaaatgc ggaggggtac atgtgcaata aaaccacacc
      961 ggacgcagcc gccgccgcac tcactacgct gatgcacgcg cttattgata ttgaatgcac
     1021 agcagagctt gcacaaaagg aagaaaggga agaatacaca ctattcgccc tggaatgtat
     1081 ccgatacacc gcaacgcggt cgctgaatga cgctaaaaat attcttgttg ctgattgtga
     1141 aaatgggggg ggggttatgc gtgatgatcg ttttaattcc ctgaaacagg aattttccgg
     1201 cgttcctgat gatgcggctg atgcgctttc gtcaatgcca gaacttatta gagcggcttt
     1261 tttcttactt tccacgagag aatataaatc aacggggctt gatgtactga atatcgccgc
     1321 cgattatgcg gaatatgtgg cagaggcgcg ttatagaaga aaatttcctg aggatgtaag
     1381 ccatgcgtga tatttaccac caaacaatag accgcgcatt tcttgcactt tctcacagtg
     1441 aaaacatgct ggaaatattg cgcatatggc ttgaaacact tggcgacaat gaacgcgaca
     1501 aacaaaaatc aagaattgcc acggcattaa taacgcttct tgatcctgta ataatggaac
     1561 tgcaagaaat agatatattg cacgacagat ataaagaaca gcacaccgga gaataaaaat
     1621 aatgaaactt aaatattctg gcttaactgc cagtggcaac actcacccta aatttacgcg
     1681 cggcgatatt taccgcgacc agtacggcgg caccgtaacg attaaaggtg tagaagagcg
     1741 acgcattacc taccgccgtg aaggctacga atatgactgc gtgatgcctg tttatcagtt
     1801 ccggcgtgat ttttctctgg tacaggcagc gccccgcagt aaaccaacca gcagggagaa
     1861 agcacgcgcc aatattcagg aaataaaaaa gatgcttaac gtattcaggg gcaagaaatg
     1921 aaaagcgcac cgaacttaaa aaaacagcct tacgacaaga tgaccgaagt catcattttt
     1981 gcgggtagtg atgcgtgggc acatgcgaaa cagtggcagg aacaggacgg gcgactggct
     2041 ggcgataatg tgcctcccgt tgtgctggct gatgatcaac tggatgaact ggcagacctg
     2101 agaatcatcg acgaggggcg ctattgtgtc cggctgtaca aggcaggcca catcaggcca
     2161 tcaaatatta atgccattgc gcacaagctg gcggcggcgg gtgtaactga tgcgaattat
     2221 taccccgaag ggatgcacag ccatatgcgg gagaactggc gcgaatacct ggaacgggtg
     2281 cgtgggaaag agccggcgga agaaaaaaac caccagcgaa aaaccacgct accgatgagc
     2341 gttggatcta ccggatacga cacgcaactg gattacgtgg ttaaggggat tattccggcg
     2401 gtatcgctat gcagcatata cggggctagc gggtcctata aatcattcct tgccggatcg
     2461 tgggcgtgcc atgttgccac tggtcgccag tggggaggcc gcagggttgc gcatggtgcg
     2521 gttctctatg tggttggtga aggcggtata ggtgttccgc gtcgtgtaaa agcctgggag
     2581 gttgtgcacg atgagcaggt gaaaaatctg tatctggtaa accgccccat ctttccggct
     2641 gccccgcttg atgttgatga aatggttatc gctgcccgtc aggtggagcg ggaaacgggt
     2701 aaacctgtac gcatgattat tctggatacg ctggcgcgtt gctttggtgg gaatgatgaa
     2761 aatgattccc gtgatatggg ggcgtttatc cgtggttgtg acgaactgaa acgacgcaca
     2821 ggggccacgg tgctggtggt tcaccattcc ggcaaggatg agacgaaagg cgcgcgcggt
     2881 tccagtgcat ttcgtgcttc gctggatgct gaataccgga tacgcaggga ggacgcagga
     2941 agcgaagcgc tggttatctc atgcaccaaa atgaaggacg cggaggaact caaagaagcc
     3001 gcatatgact tacgcgtggt ggagcttttt accgacgctg acggtgaatt aatcacgtcg
     3061 ctggtggtgg tggatgatcc gcgccctcct gttgaactgg agcgcatcga ggaggcaggg
     3121 aacaagacgg aaaaccatac cgcgctatgg gggtgcatcc gttcacgcac acagaacggc
     3181 gacaagtgca cgatcccgct gttacgtgat gacatgaaaa agctggggta tgaaatgaaa
     3241 aacttccggc gctggctgta caagctggaa aaagatgggg ttattcgtat cgatggggat
     3301 gatgtagcgc cgctataaaa gtgaggagca aaagcgaggt ggatagaaag agggccaaaa
     3361 ttagcctgct ctccctcact tttcgacctg tatacatcct caaaagtgag gggtaaaaaa
     3421 atacttatga aacacacaca tagaaaaacc gaaaatccca gctgcgacga agtgagacgc
     3481 ttgaaaaagt gaggcgaaaa agtgagaggt tgcgagaaat gacccaaaaa cgcagagaca
     3541 gaacagagcc aaaatataaa gcgttagaca tgactgagca caccttaaag gtggcaatca
     3601 gaacgataga ccgccacacg cgggaaggat acgcgaagga acatcccgac ctgataagcg
     3661 cattcatgac cacggcggcg gcaaactttg ccacgctgac agaacgggag attgccgaag
     3721 cggaacaggt aacaaccatc aacgttaaaa ccggagaggt ggaatcatga gcgaaccaaa
     3781 atttggtgaa aaactttaca agcacaacgg actgatcacg attctgcaaa taagcgcagc
     3841 tacaccaggc tggtgggttg aaacagacga aggatcctca cccgtggcat catgggcgct
     3901 ttgtgtgctt agttaccctg accgcgatgt gtatcaggat attctgcctg tgatatccac
     3961 tgacaaaggc atgaaacccg tagacatcaa aaacatgggt tttcagtgcg taatgctcac
     4021 tgaaaaaatg atggaagaga tgaaaaagaa caacgccgga tcgcttcact gaggtggcaa
     4081 tatgacagca caaattgcag cttacgggcg cctggtggac gacccgctgg taaaacagag
     4141 cagcaagggc acaccgatga ctctggcgcg tatggcggta tcgttgccat gcagccaggc
     4201 acatgacgga caggcgacgt tatggttatc ggttatcgca tttggcaaac aggccgattt
     4261 cctggctaaa catcaaaaag gcgatgttgc cagcgtatcc ggcacgatgc aggtaagcca
     4321 gtggaccgga cagaacgggg aaacacggca ggggtatcag gttattgcag acagcgtaat
     4381 cagtgcccgt gcggcacgtc ctggcgggaa cagacgcaaa accacaggca cacagggtaa
     4441 tcagccacca gcgggagacg atgaccccta cggtgatgat attccgttct gagggggtga
     4501 cgatggtaca tgaccgcata gcggaggaac tcgaggcaaa aggcttttac cggagggcgt
     4561 cggcgcgatg gggtgaagtc atgctgctgg tggagacaga caaggaacgg catcaggtta
     4621 cgatgcgacg gctggaatgt tccaggaagg cacagaggcc accggagccg ccggataatt
     4681 acggagacct gaaaaaggcg gtcgatcgca cttatgccga aatgggtatg gatggtgctg
     4741 gtgatgaaat atggcgaaat taccaggaca gctaatcaca cagccggaga aatccggctt
     4801 tttttgcacc agttgaaacg gtatggcgca ttaccgggtt ttcgtcacgg tcaggcatag
     4861 ttactatctg aaacaaacag acacaacaga ggaaaaaaca atgccgatga aatttgatga
     4921 gatattaaaa cagcgtgata aataccatgc tgacaacatg gagacgatga gcatcaatga
     4981 ttaccgcgca ttcctggaga cgggcgcact gattgaaaag gatcagcatg gttttgtgag
     5041 atgtgctctg tccggtgaaa tgctggcggt aaatcctgaa cagatagatg cattgataga
     5101 atttctgaaa gggatcaggg actgagcacg catacagccg gagcaatccg gctttttgtc
     5161 attttttgta aattatttgt tcgcggttgt tccacgttgt tcactgacag gatcggcata
     5221 ttttacccga actgaatcat gattattctc gcccgtggtg ccaggacgct ggggccactt
     5281 ttccgcctgt taatgttctc gccaatattc attaccaggc gggaaaacga tcggtgcgat
     5341 tgctgatttc cttatgaaaa acggttgagt ttttgccgcg tcctggagtt ccttacttaa
     5401 ccccaggact ttttttatgc cgagaataat cgaattacgc cagcagaaaa ccgccattaa
     5461 aaatcagatg cgcgacatgc tggagaacgc ggaaaaagaa aaccgcagtc ttaacgatgc
     5521 tgagggcgca acatttgacg aattacgcgc taaagctgaa tccctcgata aagacatttc
     5581 ccgccttgaa gccattgcag acgaagagcg cagcaagcca ggtaaaagca gtcagaccac
     5641 tgaccccgca gaactccgca actacattct gacaggtgaa acccgcgcat taagtacagg
     5701 cgttcccgct gatggtggtt ataccgttat ccctgaactg aacaccgaaa tcatgcgaat
     5761 gctggcggat gaatccacca tgcgccgcat ctgtaccgtg aagaaaatca gcagcaacga
     5821 gtttaagcag cttgtttccg ctggcggtgc gaccgttaac cacggtgaag agggtaaggc
     5881 acgcgaacag accagcaccc cgcagattaa cgaggtgagc attaagctgt atccggtcta
     5941 tgcgtacccg cgcaccacac aggaaatcgt ggatttttcc gatgtggaca tcctttcatg
     6001 gctgacgggt gagattggcg acaccttcac ggaaaccgaa gaaagcgatc tggttgtggg
     6061 cgatggtgac aaaaaagcaa aaggcttttt atccgtaccc cgtgcagaga agaacgacaa
     6121 agagcgtgat tttggtacgt tacaggtaat taaaccttcc gaatctctgg cgtggacatc
     6181 tgcggacccg ctgatcgacc tgaaatttgc attacgtaaa aaataccgca aaaacgcggt
     6241 ctgggtggtt aactccacga cggcggcaaa actccagaag gtgaagaacg cgaacggtga
     6301 ttatatctgg cgcgaccgtt tacaggcggg tgatcctgat acgttgctgg gccttccggt
     6361 cgaatatctg gagtttatgc ctgataacgt tattgccctg ggtgacttca aacgcggtta
     6421 ctacattgtt gatcacgaaa caggtgttcg caccagaccg gacaacctca cagagccggg
     6481 cttcatcaaa attttcacgc agaaatattt aggcggtggc gtggtggatt cgaacgcgat
     6541 caagattctg gaactgccac aggacgacga ttaacagcat acagaagggg cttaaaagcc
     6601 cctttagtgt tttatgggtg aaaaaattat gaagagtatg gaaatccggt catcggaaat
     6661 caccaccagc ggcagcaaca cgcttacagg ctacgttgtt cgctgggata agctttcaga
     6721 actgttatgg ggtgagtttt acgaaaaatt ccagcggggg gcgtttactg aatggcttgc
     6781 ggcgggtaat gacgttcgcg gcctgtatga gcatgaccac agtatgttac tggggcgcac
     6841 ccgttccggc acgctgaaac tggaagagga tgaaacaggg ttacgctttg aactgacccc
     6901 accggatacc agtacagggc gcgacgttat cgaactggtt aagcgtggtg atatatccgg
     6961 catgagcttt ggctttcgtt cccgtaagga tgtatgggat accacaacag atccatgcgt
     7021 gcgcaccgtg ctggttgcgg aactgtacga aattaccgtc acatccgtac cggcttaccc
     7081 tgattccggc gtggagctgg cccgccgctc cctgtacgag cagcaccccg aaaaaatgcc
     7141 gcgtgcggat aatcgccgct ggtgggcgga tttagcgggg gtgtgatatg tggcctttca
     7201 gaagaaaaaa agagcagcgc agcatgacgc ttgatgagtt tatggcgctg gctggcacat
     7261 cgaacacggg ggcgggtgag tacgtatcat cggggacagc ggaatcactg cccgccgtca
     7321 tgaacgccgt gacggtcatc tctgaggcgg tggctaccat gccgtgttac ctgtacctgg
     7381 tacgcaatga gaaggggaag gaggcccgcg agtggcttga ttctcatccg gtcgatcaca
     7441 tcctcaacga gcgcccgaac gcgtggcaga ctccctacca gtttaagcga atgatgatcc
     7501 gccactgcct gttaaacggt aatgcttacg cggtgattca gtgggggcgt gatggttttc
     7561 cggcggcttt gcatccttac ccgccgcagt cggtgaacat ggagcagaca ggagaacaca
     7621 actggcgcta ctgcatcact gacgcttaca ccggaaacac ccgcaactat ttaccgtggg
     7681 aggttctcca ccttcgttac tccacggatg acggttttat ggggcgctca cctgtaacca
     7741 tctgccgcga atcgctgggg cttgggctgg cccaacaacg ccacggcgcg agcgtgatgc
     7801 gtgatggcat gatggcggca ggggttatca cgtcaggcga atggctggac ggcgtgaagg
     7861 gcaaacaggc attagccgca ctggaacgct acaaaggggc cagaaacgcc ggaaaaacgc
     7921 ccatccttga agggggtatg agctatcagc agctgggcat gagtaatcag gatgctgaat
     7981 ggctggcctc ccgtcgcttc accattgaag acatcgccag aatgttcaac gtctcgccga
     8041 tttttttgca ggaatacagc aacagcacct acagcaattt cagcgaggca agccgcgcat
     8101 ttctcaccat gacgatgcgc ccgtggctgg cgaactttga gcagcagata aaaaacgccc
     8161 tgctggtggc ctcgcctgta cctggtatcc ggtatcaggt ggagtttgac agcgcggacc
     8221 tgttacgggc cacacctggc gaacgctttg ccacctatga gcgcggcatc aaatccggcg
     8281 ttatgtgtcc gaacgaagcc cgcgaacgtg aaggactgtc accgcgtgat ggtggtgatg
     8341 agttcagcca ggcatggaaa caggaagtaa aaatcagcga gggagaaaaa ccggaatgaa
     8401 catagggcga ctgcgtgaca gggtaacgat tcagaccctg aaacagacca gagccatgac
     8461 gggtgaaata ctcgaaacgt ggctggacgg tcacacactc tgggcaagcg tgaacatgat
     8521 cagcagcaag gaggccattt catcaggtgc agagctggcg actggaactg taaggatatg
     8581 gatacgctac cggaaggaca tcaacgccac cagtcggata aaggtcaata cggggccgct
     8641 ggcggggcgt gtactgaata tcatcgggca accgctgcca gatgccgcca ggacacgcct
     8701 tgaaattctt tgtcgtgagg gcgcggaaaa atgacagaac ttatcaccct ggaagaagtg
     8761 aaactccatt gccgcatcga tggcgacgat gaagaccagt taatcagcgg atacattgcc
     8821 gcatcgcttg aggcgtgcca gatacacata ggcaggcgct ttgatgacgg gctggagttc
     8881 acgccagcca taaagattgg ctgcatgatg tttatcgctc acctgtatga aaatcgccag
     8941 attgtcgcgg ataacgcaaa aacgcgcgta cccatgacga taggcgcact ctggacggct
     9001 taccgtgatg tgggggtgta ctgatgccgt ggcaaccatt aagacgatgt acagaaccag
     9061 gctgtaacag gcgcgttaag tccggtaagt gtgaggagca caggcgggcg gcatggcgtg
     9121 cagaggatgc cagacgagga caccgccgcg cgcgtgggta ctccagacag tgggacaaat
     9181 accgcgccat gtatctgagt aaaaacccgt tatgcgtgcg atgccttgag aaggggatat
     9241 atacgcccgc cgtggtggtg gatcacatta tccccatcga tggcggcgat gatgttctct
     9301 tctggcccga atggaaccat cagccattgt gtcaggcgtg tcataaccag aaaacgaaat
     9361 ggcttgatcc ggcaacaaaa agcaagcgtg ccgcaggtgg atttcgtgaa gatgaagaac
     9421 gggccgctaa ccgcaataac tggatgtatg acgctgatga atgagcggga acaaaaccgc
     9481 cttatccgtg gacttataag gcagcgtgac gcatggaaga cacaggaaac agggcataaa
     9541 gataaagcgt caggacgcgc agaacgcatc acagcgaagc gattaaccga ccgtgaccgc
     9601 gaggttatgg aatgtttccg caatcgctga tgaggctgta tggcggggtg gggggcgttt
     9661 tcaggacaaa cccgaccccg ccgggcaccg aacgcctccc caaattttta tgcacgggaa
     9721 ttttttgaaa aataatctga cgaaaaataa gcatggcaag accaccgaaa gcccccgcct
     9781 acctggatga catcgccgtg aagcagtggc gggaaaaatc gcggcagctt gcggaacggg
     9841 gagacctgac ccccgccgac tggagcaatc tggaactgta ttgcgtcaat tactcgatat
     9901 accgcaaagc cgtcgcagac cttgcggcgc gcgggttcag cattgttaac agtcagggcg
     9961 gcgagagcag aaaccccgca ttaagcgcga aatccgacgc tgaaagagtg atgataaaaa
    10021 tggcctcctt gcttggtttt gacccgataa gccgccgtaa aaatccaccg gaaacagaag
    10081 aagaggacga gcttgaccgc ctggaataag tacgcagaag acgtaaaaac gggcaaaatt
    10141 ccggcctgta aacggctgaa acaggccgtt aaacggtact tttcggatct taaaagcccc
    10201 ctttacacgt tcgatcgtga ggttgtggag cggtttattg ccttttccag ggtgtgcccg
    10261 cacgtaaaag gaccgatgcg tggcagaccc attgagctgg agccgtggca gcagttcgcc
    10321 tttgcgtgca tcctcggctt taaggttaag gccaccggac ggcgcaaata cacgagcgcc
    10381 tttatcgaag tgccgcgcaa aaatgcgaaa tcaacgaccg ccgcgattct ggctaactgg
    10441 tttctgatta tggagaacgg tcagcaggat atttacaccg cagcggtgag ccgtgaccag
    10501 gcgcggatcg tctttgatga tgcgcgtcag atgtgccttt tatcccgacc gttacgcagg
    10561 cgggtgaata ttcaggcgca taaggtgata cacccgaaaa gcaacagcct gttaaagccg
    10621 ctggcagcaa aagcggcaac catcgagggc acaaacccca gcctttccat cgtggacgaa
    10681 taccacctac accccgataa cggcgtttat tctgcgcttg agctggggat gggcgcacgt
    10741 cccgaagggc tgttatttgc cattaccaca tcaggcagta acgtcgtttc agcctgtaag
    10801 cagcactatg attattgctg ccagatcctg gacggcgaag aggtcaacga ttcaattttt
    10861 gtactgattt acgaactgga cgacgaaaac gaggttgatg atccggcgat gtggataaaa
    10921 gcgaacccta atattgatgt ttccgtggat cgtgaaaaac tggcctcaac catccagaaa
    10981 gcgcggggta ttccgtcgca gtgggtggag atgatgacaa agcgatttaa tatctggtgt
    11041 caggggacta ccccgtggat gggtaacggc gcatgggctg agtgtgccgg aacgttcacg
    11101 gaggaagatt tacacggcca ggagtgttac gcggggctgg acttatcatc aaccagcgat
    11161 atttccagcg tgtgctatgc ctttccggtc ggtaaaacca ttatgctggt ttcccgtcac
    11221 tatctgccgg aatttcagct acagaacccc gccaataaaa accgcgccat ctatcgtcag
    11281 tgggctaaag caggctggat acgcacaacg cctggcgact gcattgatta cgaccggatc
    11341 agagatgaca tcatgcagga cgcggagaaa tttaatatca ggctggtggg ctttgatacg
    11401 tggaacgcca cgcatctcag aacgcaatta cagggggcag gttttgaggt ggagccgttc
    11461 ccgcaaacct acctcagatt cagtccggcg gcgaaatcgt tcgaagtttt tgttaaccgc
    11521 agggtgattg tgcatcgtgg cgatccggtg ttgtcctggt cgatgagtaa cgtcgtgatg
    11581 cagagtgacg cgaacgccaa tatcaagccg aacaagaaaa aatcaccgaa caagatagac
    11641 ccgagcgtgg cggcgctgat ggcgtttggt acattccagg cagagcatga ggattttgct
    11701 tttgatataa gcgacagcca ccgccagaaa ctggaagaat ttagcggggt gtaatgaggt
    11761 cagcagcatg acagaagccg aaataacggg attaatccgc cgcgttgccg gaatcagcca
    11821 gcaggctgac gaacaggcca cgcagccgga cagcgtgaca gccgaaaact atgcgcgtgt
    11881 tgttgctgag gtgatgcgcc gtgatggtat agaacttaat ggcgtggata tgcgcaacat
    11941 acgaaccaga gctcttgagt tgctggccta ccgtcgccgc gtggagatgt atcgggagaa
    12001 ggagaaaata acgtaccact ggaagaagcc ggagcggttg cggcggtaac tggttgatat
    12061 tcccgacgga ccaaaaatgg gtggctacat ccctcgcttc tgaggactgt taacaagctg
    12121 cctgaaagac agtcacaaac tacgaaattt tcgcagttta attaatgatc agcttaatcg
    12181 ctaacccgct gatattttcg gaaacctcaa tttgaggaag tcggcgcggt aactcgctga
    12241 actttaagca aagtgcaaaa ttgcgcttgc tgaataatca ttatgattac gcagatgatt
    12301 aaggaatgac cgaaggcgga aattcgcctg tggttaatgg gggagttgca gatctgcaac
    12361 tcgaccatga aactacggaa actacccgta gtttgggtag taagagtaac acccagattt
    12421 tgggtcttac tcgtgatacc caaatttggg gtatcagtgg caaccataac gactttcgtt
    12481 acggttgatg cttttacccc attgggggaa aggtattacg ataatcataa cacctaccga
    12541 acaggtaaag cccaccagcc tgattaacgt ttaaccggaa aaaaattccg gtaggtgggg
    12601 atccccatat cgacattaac gcccctcatg aattggtgcg cttccccctg gaaagatgat
    12661 ccgcctgtat atttcttgtg tctatttgtt ccacgttgtt tcatacagtg caccgaacgg
    12721 tgtagtcact ggtgtagtca ctttgcgatt tttggcactt tttgcggtga aaattattta
    12781 attaaatcaa cgtgttatgt gaaataagtt atatataaca gcaataaata atgcgagaat
    12841 aaacggcacg atgatctcgg cggcaaaacg gataccgcag agaataatga ccagcattcc
    12901 caacataatg acgattttta ggccattgag cgtgatgatc ggctttgcca tgcttgtccc
    12961 cgttttttct tcctttgcta ataataatgt ctttttcgct ccgtcatcat aacgaaaatc
    13021 aaaccgctca ggtaaagaag tgaaaatatt ttgagttaat tcttaagcta tgatacaaat
    13081 caggcgtgtt caactaccga ggacaattat catccgcgat gacgagaagc aacactgcgg
    13141 ataattgtaa tattatggac aatttgttca ggacgttatt ttcaacattt acccatcttc
    13201 gtacctcatc cactatcttg ctcgtaggtg agcagcactg gcgcaacgcg ctttagtcct
    13261 ttcttctgcc taagcttgcc ctatgcgcgg ggcttgacca ctttactttg gtttcgctga
    13321 attaagcgaa aattaaaata attctcttgc aggagaagga caatgtatat ttattggatt
    13381 ttattaggcc tggctattgc tacagaaatt accggtacgc tgtcaatgaa atgggcgagc
    13441 gtcagtgagg gaaatggcgg ctttatttta atgctggtga tgatttctct gtcgtatata
    13501 tttctctctt tcgccgttaa aaagatcgcc ttaggcgtag cttatgcgct gtgggaaggt
    13561 atcggtattt tatttattac cttgtttagc gttttgttat tcgacgaaag tttatcgctg
    13621 atgaaaattg ccgggttaac caccctggtc gccgggattg tgttgatcaa atcaggtacc
    13681 cgtaaagcgc gtaaacctga actggaagtg aaccatggcg cagtttgaat gggttcacgc
    13741 cgcctggctg gcattggcaa tcgtgctgga aatcgttgct aacgtctttt tgaaattttc
    13801 tgacggcttt cgtcgcaaaa tatttggctt gctctccctg gcggcggtgc tggctgcctt
    13861 tagtgcgctt tctcaggccg taaaagggat cgacttgtct gtcgcttatg cattgtgggg
    13921 cgggtttggt attgccgcca cgttagccgc aggttggatc ttgtttggtc aacggttaaa
    13981 tcgtaaaggc tggattggcc tggtcttgct gttggctgga atgatcatgg tgaaacttgc
    14041 ctgatgaaca cgctgcccgc actgtcgggc agcgtttgaa cattattttt gcgacagttg
    14101 atccagcttg tcgcgaaaac cggtaacaga aatggcccgg ttatcggcgc gccagcgatc
    14161 tttcgcggca ggagccgaac tttgcacccc aattaattgc cagccgtcat cggtatgcaa
    14221 catcaaaggc gaaccgctgt cacccggcaa ggtatcgcac tgatgtgaca tcaccgacgt
    14281 ttgcgcccag ccagtcactt cacagttttg atgactgtat aacgtatcga gatgatcttc
    14341 aggatatccc gcctgagtca ctttacgacc agccgatttt aatgcggcag taagcgcggc
    14401 tttatctccc tcaaataacg gcaacggtgt aatgccagaa gggggattac gcagcacaat
    14461 caagccgaag tcccatggtg cagctgcggg aggaacaatc caaccatccc catctgcttt
    14521 taaccgcttt cccagtgtcg gatcaacgcg gccttctatg tcgtggatgt catagcgcca
    14581 aagaccttta tttgacacaa aacgcagcgc taccgcctta tccgctttac ccttaggcgg
    14641 cgtcaataaa cagtgtcctg ccgttaatgc cagattgggt gcaatcagcg tcgccgtaca
    14701 taaattgccg ctggccgttt ccagttgccc aaccgcatcc cacggtgatt gggtcgtgtc
    14761 attcactggc acacgatcat catgaccaaa aaacagggtg ctgacctcat cgtttgccga
    14821 tttggcaacg tctggtttat ctgcaaacac aaaagcagac gtcaaactaa ttgcacccaa
    14881 cactacagca atggttgtac gcatatcaca ctctggtggg taattatgat tattaaaagc
    14941 aaaccctcat aaatactata gacgggacgg gatgaaagtg ggagtaaaat cagataacta
    15001 cagtcaggaa agataaaaac gggcggcaat cagtgcgcat aaaataagga tgataagaat
    15061 gaactcaaag cgataacggc ccaccatact accctccgct aaaggcggtg ctaaacgtta
    15121 cctgcttagc accgtaattt tcaggcgcga gggggcgcgc cagcattact gttgaaaact
    15181 tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat ggctgtgttt cccggcgtgt
    15241 ttcttcgctg cctgcgcttt ttgttcaggg gctttctgtt cagctttcgt atttttatga
    15301 tgctttttag ccgcctgcgc tttctgggca ggtgctgctt tatgctgttt tttatgatgt
    15361 gtcgttttcg ccggcgctgc tttggtggtc gtcgcagtcg gagcaggtgt ggtcgcagtc
    15421 tctgcagcaa aggcggcaga agacagaccc atagcagcgg caacaaccag agctaatact
    15481 tttttcattg tcataccctc aatttgtttt ttcatttaac cccactgcgg ggccgttgaa
    15541 ataactatat ccctgggaat ggcagacttc cgtgagtggt tggtttcagc gtgtaacgat
    15601 atgtacaaac gctgaataaa ttacagcgtt gataataacg tattgtgact taagggaaat
    15661 ttagctacca ataatagtag tcttgatcgg gttaactatt ttactactta caaagcggta
    15721 gaataactgc gcatcagtaa taaatgacac acagcaaaat gaatccgttt atttgggtac
    15781 ttataatcct gatgacgcta gacgcgctgc gggaattggc tggcgcttcg tctattttag
    15841 gatggctatt aacgttggtt tgagcaagca ataagtccgg acgtgtagtt accgcagtcc
    15901 gggcttattt ttcaggcgtg cagacgacga tgcaaacgcg tcccgaccag cagagcaatg
    15961 accagcatca gagcaataaa tgcgccgacg ccgttccagc catagttatg ccagaaaaca
    16021 ccacccagcg tcccggcaat actcgacccc agatagtaac tgaacagata cagcgaggag
    16081 gcctggcctt tagcgcgttt tgcgcggggg ccaatccagc tgctggcaac tgagtgggct
    16141 gcgaagaatc ctgctgagaa gagtaacatt ccggcaaaga tcagccacag cgagctgaat
    16201 aaggtcatca gtaaaccaaa cagcataacc cccgtcgaaa acaacatcac cggaccgcgc
    16261 ccgtagcggg tggtcatggt tccggctttg ggtgagctcc atgtaccggt caaataagcc
    16321 agcgataata agccaaccac ggcctgactg acatgccagg gggagagcat caaccgatat
    16381 ccgatgtaat taaacagcgt gacgaacgac cccatcagca aaaagccttc tgcgaacaat
    16441 aacggtaatc cccggtcacg ccagtgcaga cgaaagttga taaacaacgt tttaggtcgc
    16501 agcgaagtcg ggcgaaaatg gcgtgattca gggaggattt tccagaacat caacgccgag
    16561 gctagcgcga aacaaccgat tgccgccaga gcaattcgcc agttgaaaaa gtccgtgaag
    16621 acaccactaa ttaagcgtcc gctcatgccg ccaattgagt tgccgctgat atacaacccc
    16681 atcgaaaagg ccacgaaact ggggtggatt tcctcgctaa gataagtcat gccaacagct
    16741 gccacgccac ttaacgaaag cccaatcaag gcgcgcataa tcaaaatgcc gtgccagctg
    16801 gtcatcattg tcgaaagtaa cgtacaaatg gaggccaaca gtagcgccgt gaccatcact
    16861 ggtttgcgac caatggcatc ggataggggg ccagtaaaca gcaaaccaat agccaacatc
    16921 gccgtggaaa tggacagtga aatactactg ttcgcgggcg ttaagccaaa ctcctgcgaa
    16981 agcaccggaa ggataggctg cacacaatag agaagtgcaa atgttgccag tccggcagag
    17041 aacagcgcca gggtgacgcg cataaattgc ggcgtaccgc gtttaataaa ttgatttggc
    17101 tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc catcaacagt tgtagtacgg
    17161 ctcacttgaa atccttgcta aatatgcctg tggatcaggc ttatacatag gttaggaaaa
//
LOCUS       DAZFRJ010000089        17200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000089 DAZFRJ010000000
VERSION     DAZFRJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.089"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..586
                     /locus_tag="ACHKUN_002714"
     CDS             2..586
                     /locus_tag="ACHKUN_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6660947.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            666..1361
                     /locus_tag="ACHKUN_002715"
     CDS             666..1361
                     /locus_tag="ACHKUN_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6660948.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1391..3907
                     /locus_tag="ACHKUN_002716"
     CDS             1391..3907
                     /locus_tag="ACHKUN_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6660949.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3918..5009
                     /locus_tag="ACHKUN_002717"
     CDS             3918..5009
                     /locus_tag="ACHKUN_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6660950.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5052..5912)
                     /gene="rsmI"
                     /locus_tag="ACHKUN_002718"
     CDS             complement(5052..5912)
                     /gene="rsmI"
                     /locus_tag="ACHKUN_002718"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO6660951.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5976..8012
                     /gene="lpoA"
                     /locus_tag="ACHKUN_002719"
     CDS             5976..8012
                     /gene="lpoA"
                     /locus_tag="ACHKUN_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO6660952.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7970..8365
                     /locus_tag="ACHKUN_002720"
     CDS             7970..8365
                     /locus_tag="ACHKUN_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO6660953.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8385..8975
                     /gene="diaA"
                     /locus_tag="ACHKUN_002721"
     CDS             8385..8975
                     /gene="diaA"
                     /locus_tag="ACHKUN_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO6660954.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8985..9560
                     /gene="dolP"
                     /locus_tag="ACHKUN_002722"
     CDS             8985..9560
                     /gene="dolP"
                     /locus_tag="ACHKUN_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO6660955.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9674..10714)
                     /locus_tag="ACHKUN_002723"
     CDS             complement(9674..10714)
                     /locus_tag="ACHKUN_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6660956.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10787..11422)
                     /locus_tag="ACHKUN_002724"
     CDS             complement(10787..11422)
                     /locus_tag="ACHKUN_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6660957.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11550..12068
                     /gene="yhbO"
                     /locus_tag="ACHKUN_002725"
     CDS             11550..12068
                     /gene="yhbO"
                     /locus_tag="ACHKUN_002725"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO6660958.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12048..12491)
                     /locus_tag="ACHKUN_002726"
     CDS             complement(12048..12491)
                     /locus_tag="ACHKUN_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO6660959.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12542..12844
                     /gene="yhbQ"
                     /locus_tag="ACHKUN_002727"
     CDS             12542..12844
                     /gene="yhbQ"
                     /locus_tag="ACHKUN_002727"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO6660960.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12831..13334)
                     /locus_tag="ACHKUN_002728"
     CDS             complement(12831..13334)
                     /locus_tag="ACHKUN_002728"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6660961.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13328..13852)
                     /locus_tag="ACHKUN_002729"
     CDS             complement(13328..13852)
                     /locus_tag="ACHKUN_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO6660962.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14061..15056
                     /locus_tag="ACHKUN_002730"
     CDS             14061..15056
                     /locus_tag="ACHKUN_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO6660963.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15065..15943
                     /locus_tag="ACHKUN_002731"
     CDS             15065..15943
                     /locus_tag="ACHKUN_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO6660964.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16149..17155
                     /locus_tag="ACHKUN_002732"
                     /pseudo
     CDS             16149..17155
                     /locus_tag="ACHKUN_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
BASE COUNT         4334 a         4560 c         4352 g         3954 t
ORIGIN      
        1 aatgaataaa gttacaaaaa cagctattgt gggcttactt gcgcttttcg caggtaatgc
       61 cgctgcaacc gatggtgaaa tagtttttga tggcgagatt ttaaaatccg cttgtgaaat
      121 caatgactct gataagaaaa ttgaagttgc tcttggtcac tataatgccg aacagtttcg
      181 ttctgttggc gatcgtagcc caaaaattcc attcactatt cctttagtta actgcccagt
      241 gactgggtgg gagcacgata acggcaacgt tgaagcgtct ttccgtttat ggctggaaac
      301 gcgggataat ggcaccgtcc ctaacttccc taatctggca aaagtgggct cttttgctgg
      361 cacagcggca acaggtgtgg gtatccgtat tgacgatgcg gaaagcggaa accttatgcc
      421 actgaatgct atggggaatg ataacacggt ttatcagatc ccagcggact ccaatggtat
      481 tgtcaatgtt gacctcatcg cttactacgt atcaaccgtt gaagcatcag aaatcacccc
      541 aggggaagca gacgctgtcg ttaacgtaac gctggattac cgttaatacg ttacggcgtt
      601 atctgacctt tcagataacg cccttttcct tcctctttct cgttgtatca ggttgaaaaa
      661 taactatgtc aaaacgaaca ttcgcggtga taataacctt gttgtgtagc ttctgtatcg
      721 gccaggcgct tgcagggggt atcgttttac agcgaacgcg agtgatctat gatgccagcc
      781 gcaaagaggc tgcgttacct gtcgcaaaca aaggcgcaga aacgccttat ttactgcaat
      841 catgggtaga taatatagat ggtacaagcc gtgccccatt tattataacc ccaccgctat
      901 ttcgtcttga agccggcgat gactcatcac tgcgaattat taaaacagct gataacctgc
      961 ctgaaaataa agagtcgctg ttctacatta atgttcgtgc cattccagca aagaaaaaat
     1021 cagatgatgt taatgctaac gagttgacgc tggtatttaa aacacggatc aaaatgtttt
     1081 atcgccccgc acacctgaag ggacgggtaa acgatgcgtg gacatcactg gaatttaaac
     1141 gtagtgacca ttcactcaat atatataacc caactgaata ttacgtcgta tttgccggac
     1201 tggcagtcga taaaaccgat ctcacaagca aaattgaata tatcgcgccc ggagaacata
     1261 aacagttacc acttcctgca tctggcggaa agaacgtgaa atgggctgca atcaatgatt
     1321 atggcggcag ttccgggaca gaaactcgtc cactgcaata acaaatataa aaaacacagg
     1381 tcatcaggga atgccacaac gacaccacca gggacataaa cgcacaccga aacagttggc
     1441 gctcatcatc aaacgctgtt tgccgatggt gctcactggc agcggcatgc tttgcactac
     1501 cgctaacgcc gaagagtatt atttcgaccc cattatgctg gaaactacaa aaagtggtat
     1561 gcaaacaact gatctgtcac gtttttcaaa aaaatacgca caactaccag gaacttatca
     1621 ggttgatatc tggctgaata aaaagaaggt ttcacagaaa aaaattacat ttaccgccaa
     1681 tgcagagcaa cttctgcagc cacagtttac ggtagaacaa ctacgtgagc tgggtattaa
     1741 ggtggatgaa atcccggcgc tggctgaaaa agatgacgat agcgtgatca actcgcttga
     1801 acaaatcatt cccggtacag ctgctgaatt tgatttcaat catcagcgac ttaatttgag
     1861 cattccccaa attgcactgt accgtgatgc aagaggttac gtctcccctt ctcgttggga
     1921 cgatggtata ccaacgctgt ttaccaacta ctcgtttaca ggttctgata accgttaccg
     1981 ccagggcaat cgtagccaac gacagtacct gaatatgcaa aatggtgcta attttggccc
     2041 ctggcgatta cgtaactatt ctacgtggac acgcaacgat caggcgtcaa gctggaacac
     2101 tatcagtagt tatttacaac gtgatatcaa ggcgttgaag tctcagttgc ttctgggaga
     2161 aagcgccacc agcggcagta ttttttccag ctacaccttt actggcgtgc aactcgcttc
     2221 cgacgataat atgttgccaa acagccagcg cggatttgcc ccaacggtac gcggtatcgc
     2281 aaacagtagt gcaatcgtga ctatcaggca aaatggttat gtgatctatc aaagcaacgt
     2341 gccagcgggt gcctttgaaa ttaacgatct ctacccctct tccaacagcg gcgatttaga
     2401 agtcacgatt gaagaaagtg acggtacgca acgtcgcttt atccagcctt attcttcatt
     2461 acccatgatg cagcgacctg ggcatctaaa atatagcgcg accgctggac gctatcgcgc
     2521 tgatgcaaac agtgatagca aggaacccga atttgctgaa gccacggcaa tatatggttt
     2581 gaataatact tttacgctgt atggcggcct gctcggttct gaagattatt atgcgctggg
     2641 gatcggtatc ggcggcacac ttggcgcact gggcgcgttg tcgatggata tcaacagagc
     2701 tgacacccaa ttcgataacc ggcactcttt tcatggctat caatggcgta cgcagtacat
     2761 caaagatatc ccggaaacca acaccaatat cgctgttagc tactatcgct ataccaacga
     2821 tggctatttt agttttgatg aagccaatac ccgtaattgg gactataaca gtcgccaaaa
     2881 aagtgaaatt caattcaaca tcagccagac aatatttgat ggggtaagtc tgtatgcctc
     2941 cggttcgcag caagactatt ggggcaataa cgataaaaac aggaatatct cagttggggt
     3001 ttccggtcag caatggggaa ttggttacag cctgaattat caatacagtc gctacacgga
     3061 tcaaaataat gaccgcgcac tctctttgaa tctcagtatt ccgttagaac gctggttacc
     3121 gcgtagccgg gtttcctatc agatgaccag ccagaaagat cgcccaaccc aacatgaaat
     3181 gcgtcttgat ggctcactgc tggatgatgg tcgcctgagc tatagcctgg aacaaaatct
     3241 ggatgacgat aacaaccata acagtagcct gaacgccagt taccgttcac cttatggcac
     3301 cttcagtgcc ggatacagct acggtaatga cagcagccaa tacaattacg gcgttaccgg
     3361 cggcgtggtt atccatcctc atggcgtgac gctctcgcaa tatctgggca acgcttttgc
     3421 gcttatcgat gctaatgggg cttctggcgt gaggatacaa aactatccgg ggattgctac
     3481 tgatcccttt ggctatgcag tggttcctta tctcacgact taccaggaaa accgtctctc
     3541 ggtagatact acgcagctgc ccgataacgt cgatcttgag caaacaacac agtttgtggt
     3601 gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact
     3661 tgttacagtc agcgatcgca acggtaaacc gttgcccttt ggcgctcttg ccagcaacga
     3721 tgagacgggg caacaaagta tcgtcgatga gggcggcata ctatatctct ctgggatatc
     3781 gagtaaatca caaagctgga ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt
     3841 tgcttttagt acaccggatt cagaaccaac aacctctgta ttacaaggca cggcgcagtg
     3901 ccattaagga taaaaaaatg aaaagagcgc ctcttataac aggacttttg ttgatatcca
     3961 catcctgcgc ttatgcctcc tcaggagggt gtggagctga cagcactagc ggtgcgacaa
     4021 attacagcag tgtggttgat gatgttacgg tgaaccagac agataacgtg acaggacggg
     4081 agtttacctc tgccacgcta agtagcacta actggcaata cgcctgtacc tgctctgcgg
     4141 gtaaggcagt taaacttgtc tatatggtca gccctgtact taccaccact ggacatcaag
     4201 caggatatta caaactcaat gatagtctgg atattaaaac cacattacag gcgaatgaca
     4261 ttccaggact cgtgactgac cagaccgttt ctgttaacac ccgattcaca cagataaaaa
     4321 gcaacaccgt atattctgct gcaacccaaa cgggtgtttg ccagggtgac acgtctcgtt
     4381 atggacccgt taatattggt gcaaatacca cctttaccct gtatgtcacc aagccatttc
     4441 tcggctcgat gaccattccg aaaacggata ttgccgtcat taaaggcgca tgggtcgatg
     4501 gaatgggaag cccgtctaca ggtgacttcc atgatttagt caagttatcg attcagggaa
     4561 atctcaccgc cccacagtcg tgcaaaatta atcagggcga tgttattaag gttaattttg
     4621 gattcatcaa tggtcagaag tttaccaccc gcaatgccat gccagacggt tttactccag
     4681 tagactttga tatcacttat gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa
     4741 tgcgcatcga cggtacaact ggggtagtag accagtacaa cctggtcgcc agacgaagaa
     4801 gttcagacaa tgcgcccgat gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa
     4861 atattccttt tcagaacggt atccttcccg ttgatccttc cgggcatggc accatcaaca
     4921 tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga aacaggaaaa tttcagggca
     4981 ccgccaccat taccgtcatc gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg
     5041 cttcggcact cttacccctg ctgctccagc gcatacttat acagcgcatt tttcttcacg
     5101 ccgtgaattt ctgccgccag cgccgccgct tttttcaacg gcagttctgc ctgtagcagc
     5161 gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct gtgctttatg accttcgata
     5221 atcagcacca tttcgccttt gcgacggttt tcatcttctt ttacccacgc cagcagctcg
     5281 ccaacgggcg cgccgtgaat ggtttcccag gttttggtca gctcacgcgc cagaaccacg
     5341 tagcgggatt cgcctaatac cgcaacgata tcttccaggc tatctaacag acggtgggtg
     5401 gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg
     5461 ccttttgatt tggcaggtaa aaagccttcg taacagaaac ggtcagaggg taaacccgcg
     5521 gcgcttaacg cagtgatagc agcacacggc ccgggtagcg gcaccacgca aatccccgct
     5581 tcacggcagg tacgcaccag atggtagcca ggatcgttaa ttagcggcgt tccggcatcg
     5641 gaaaccagcg caatgttctg cccttcttgc agcttcgcca gcagcgtttc ggctttttgt
     5701 tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat
     5761 aaaccggtgt gacgagtatc ctcggcggca atcagatcaa cggcctgtaa tacctctaac
     5821 gcacgctggg tgatatccgc cagattgccg attggcgtcg gtacaatgta aagctggccc
     5881 tgagaattat ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat
     5941 tgagcattgc gtaaaaaaat atcactggat acattatggt accctcaaca ttttctcgtt
     6001 tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc ggttgtggca
     6061 cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct gattctgcct
     6121 tttatcttca gcagatgcag caaagctctg atgataccag gatcaactgg caattactcg
     6181 ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac
     6241 taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg gtagagatta
     6301 aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg
     6361 atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc
     6421 gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctc ggcgcgaaag
     6481 aaaaacagca gaatattgat gccacctggc aggcgctctc ctccatgact caggaacagg
     6541 cgaatacgct ggtgatcaac gccgacgaaa atattctgca aggctggctg gatctgcagc
     6601 gcgtctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc gccgactggc
     6661 aaaaacgtta tccgaacaat ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa
     6721 aagcgtttaa accagcctcg accaacaaaa tcgccctgct gttgccgctg aatggccagg
     6781 cagcggtatt tggtcgcact attcagcaag gctttgaagc ggcgaaaaat atcggcactc
     6841 agccagtggc ggctcaggta gctgccgcac ctgccgcaga cgtagctgaa caacctcagc
     6901 cgcaaaccgt ggatggcgtt gccagcccgg cacaagcctc ggttagcgat ctgaccggtg
     6961 aacagcctgc ggcacagcca gcgcctgtaa gcaccccggc agcaaccacc acagcggtaa
     7021 gcgcgccagc gaatccatcc gcagaactga aaatctacga tacctcatca caaccactta
     7081 gccagatctt aagccaggtt cagcaggatg gcgcgagtat tgtggttggt ccgttgctga
     7141 aaaataacgt tgaagagttg cttaagagca acaccccgct gaacgtgctg gcactgaacc
     7201 agccggagaa tatcgaaaac cgcgtcaata tttgttactt cgcgctttca ccggaagacg
     7261 aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg ctggtgctga
     7321 tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt tgcgcaggag tggcagaaac
     7381 tgggcggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta cgcgcgggtg
     7441 ttaacggcgg ttctggtatc gctttaacgg gtaccccgat tactcccaga gcgacaaccg
     7501 actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat gaccagttca
     7561 ccaataatgg cggtcgtgtc gatgcggtgt acattgtggc aacgccgggt gaaatcgctt
     7621 ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca
     7681 gctcccgcag tgcgcaaggg accgctggcc cggatttccg actggagatg gaaggcttgc
     7741 agtacagcga aatcccgatg ctggcaggcg gtaatctacc gttaatgcag caggcactca
     7801 gcgcggtgaa taacgattat tcactggctc gcatgtatgc gatgggcgtc gatgcctggt
     7861 cgctggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc aacggtaata
     7921 ccggaagcct gacggcaaac ccggattgcg tgattaacag gaagttatca tggctacagt
     7981 accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg atgcgtggga
     8041 agtacaagcg cgtcgctggc tggaaggcaa aggactgcga tttgtcgccg ctaacgtgaa
     8101 cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc cggaccaccg tttttgtcga
     8161 ggtacgctat cgccgctctg cgctttatgg cggcgcggca gccagtgtga cccgcagcaa
     8221 acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg ggagttttga
     8281 tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg agtggattaa
     8341 ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa gaaagaatta
     8401 aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg cttccggatg
     8461 ccatctcccg tgcagccatg acgctggttc agtctctgct caatggcaac aaaatcctct
     8521 gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc atgatcaacc
     8581 gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat aatgttgtct
     8641 taacggcgat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc
     8701 tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc cgcgatattg
     8761 taaaagcggt agaagctgcc gttacgcgtg atatgactat tgtggcattg accggctatg
     8821 acggcggcga acttgcaggt ttgttagggc cacaagatgt ggagatccgc attccttcgc
     8881 atcgtagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg tgcgatctga
     8941 tcgataacac gcttttccct caccaggatg tttaaggaga atacatgaag gcattatcgc
     9001 caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg
     9061 gtaccgctgc tgtgggtacc aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg
     9121 acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga
     9181 aagaagcccg tattaatgtc acggcgtatc agggcaaagt gctgctggtt gggcagtcac
     9241 caaacgctga actttcggct cgcgccaaac agattgctat gggcgtagac ggtgccaacg
     9301 aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagcg tctaacgata
     9361 cgtggatcac caccaaagtg cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca
     9421 acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctggtg actgaacgtg
     9481 aagcgaaagc ggcggcagat attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag
     9541 cgtttacgtt tattaaatag cagccccttg taatgcctga tgcgacgctt gccgcgtctt
     9601 atcaggccta caacgatgca aaccgtaggt cggataaggc gtttacgccg catccgacat
     9661 gttttccctc aaatcagaac aacagcgcca gcccgccgac aatcacacca gacactgcca
     9721 ccatcgcccc tgtcagccat aaggctttcg ctgggaacgc tttgcgcagc ataatcagtg
     9781 acggcaaact caccgccggg agcgtcatca acaatgccag cgccggagca gttcccatac
     9841 ctgccagcat catcgtttgt acaatcggaa tttctgctgc cgtgggaatg acaaacaagc
     9901 atcctgctac cgccatcgcc atcacccaca tcaggctgtt atcgacagca ccatcggcat
     9961 gggggaataa ccagacgcga gcggcaccca acaccagtac tgcaaggatg taaaccggga
    10021 tcgtactcca gaaaagcgtc catagcgccc tgccccagcg gctaaaaaaa ccgccctgtg
    10081 cttccggtat gtcaatttcg accggtgcct gcgtttgcgg tgtttcacgc acccattttt
    10141 gcaccagcgt cgcaatcagc aacaccatca ccagcccggc caccagacga atcgccgcaa
    10201 aaccccagcc gaggacaaac cccataaaca ccagcgtcgc cgggtttaac accggattgc
    10261 ccatccagaa tgccagcgca ccgcccatcg acacctgttg gcgacgcatt cccgccgcga
    10321 ccggagccgc acagcaggta cacatcatgc ccggcagcga aaacagcgtt cccagcagcg
    10381 tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg atcagcacct
    10441 gaatcaacga accgaggatc acccccagca ctgccgcttt ccataccgcg aggaaataga
    10501 gcatcgcgta atccaacgcc gcctgccacg gattagcatc cgcctgcgca aggatagatt
    10561 taccgatact gtgggtttcg gcagcagtaa acgctttgcc gtagtaaggt tgccatttca
    10621 cataccagag accggcaatg acaacgagaa agaaaagcgc gggtttccac cactgaatgg
    10681 gcgttgccgc ctgagatgaa gactgaccag tcatagcatt ccccaggaat aagttatgat
    10741 aattgagcgc gtgaatatta cgctcactat caattcttgg gaataattat tcagctcttt
    10801 tgcgtaattc tgaagagctt aaaatcgtca caccttcatg cccgggccgc atcgcctctg
    10861 ccagcatgac ccgcgccacg tctctcgcat caatggattt ccagttaccg ggtaataaac
    10921 ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg cccagtaaca
    10981 tcgacgggcg agcaatggtc aatttcggcc agttctgggc gattaatgct tcttccatct
    11041 cccctttgac gcggttatag aaaaacggcg agtgggcatt ggcacccata gcactgacca
    11101 ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc ggtatccact accagcgtgt
    11161 aatcggcatg aataaacgcc tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac
    11221 aaaacacaat gtcgatggga tcggtgacct gcgccagcgc gtcagtcagt tgcggatcat
    11281 ggggattaaa caccccgggc atatcgccca acggacgtcg cgtcggcgcg gctatggcgt
    11341 taactttcgg ttcgttaatc aacatccgca gcaggtgacc gcccaccagc cccgttgcgc
    11401 ctgtaatcag tacctgactc atcttcgctc ctttacagaa ttgtctgcct tgcgctccac
    11461 ggctcatgca ccaggcttaa tagaccatga ggtaattatc cccgattgtg ggaaattcgc
    11521 ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa aattgccgtt ttaatcactg
    11581 atgaatttga agattcagaa tttacttcac ccgcagacga gttccgtaaa gccggacacg
    11641 aagtgattac cattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca
    11701 gcgtgaccat cgataaatcc atcgatgaag tgacgccagc ggagtttgat gccctgctgc
    11761 taccgggcgg ccattcaccg gattatctgc gtggcgacaa ccgttttgtc acctttaccc
    11821 gtgattttgt gaatagcggc aaacctgttt tcgccatctg tcacggtccg cagctgctga
    11881 tcagcgccga tgtgattcgg gggcgcaaac tgaccgcggt taaaccgatc attattgatg
    11941 ttaaaaacgc gggcgcggaa ttttacgatc aggaagtcgt ggtcgataaa gatcagctgg
    12001 ttaccagccg gacaccggac gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg
    12061 gtgcctgagc cgcgtagcca aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat
    12121 ttgatttcat cgagccggat ttcccacacc ggggccgaca gcattctggc aaccggaaag
    12181 cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac cttccagcct gcggatctca
    12241 cctttaaact gcacgccgcg aattaacgct accgttttcg gctgaccgtt taccgttccg
    12301 gcaaccgcag cctgcggccc actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg
    12361 tagaaggcga ctttctgcgc atcaaacaga taaaaagcat tagcgcacca gagttcccct
    12421 tcctgctgca cacaccaggt gacaacatgt tgttttgcca accaacggct gatggtgatg
    12481 agtgtttcca ttgctgttct cttttatact gtgggcctga ccttaacatg cgacgcagac
    12541 gatgacaccc tggtttcttt acttgatccg taccgccgac aataagcttt ataccgggat
    12601 caccacggat gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg cgaaagcact
    12661 gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca gtgggcgatc gttcgctggc
    12721 gttacgggcg gaatatcgcg ttaagcaact gaccaaacgg caaaaagagc gtctggtagc
    12781 ggaaggcgca gggtttgcag agctgttaag cagtctgcaa accccggaga ttaaaagcga
    12841 ttgaaatgct cgtgatactc aaccagacca gttacgccat tcaacgcgtc atccgctaaa
    12901 cgatgtacct ggaaggcgct ttcagtgcct ggccagcggc aacgcaggtc gtgatgtgcc
    12961 gccagttcaa agccaaaacg gctgtacagc gccggatcgc ccagcgtcac cactgcggca
    13021 tagccgaact cattaagcga atcgagtcct tcatagacca gttggcgtgc cagcccctgt
    13081 ccacggtatt tttcatcaac cgccaacggt gccatgccga cccattgcag gtcttcgccc
    13141 tgcacatcaa ccggactaaa tgccacatag ccaatgacct gaccttcgtc atctgtcgcc
    13201 accagcccca gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt cgcttccgca
    13261 tcactttcga atgagcgacg tagcagggca tcaatacccg gcgcatcaat gggaatttct
    13321 actcgaatta gcatggttca cctaccgatg tctgtttggt ttcaggcgcg gttttcattc
    13381 ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg taaggctttc ggcatttgct
    13441 ccagttcaat ggcgtccatc aggtttttca catacagccc cagctccgta tcgccttcaa
    13501 tcaccagccg acgctggaag aagagcgtat ccggatcttg tttacgcgcc gcaatcatca
    13561 gcagatcgct ggcgtcggca ctaaaactca catcagcttg cgcgttctgg ctgaccacca
    13621 gtttgccatt caccaccgag gtaaaccatt gcaggtcaat atcacgcaca tgaatactta
    13681 accagcggcc ttcaagaaac tccagctcgc catcatccag cgcctgacgg aattgccagc
    13741 ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt accggtacac
    13801 tcaacagaga tggccccaaa tgcacaatac gggaacgcag tttatccaac acgagtttta
    13861 ctccctgttt caacaatcat cctattttgc catatcagaa aaataacata gcggtataaa
    13921 tcaacaattc catatgaaat tgctgctacc accaatacaa ctttaactgc cttaaatcaa
    13981 aaattgtcgc agcaaggtta actaaaatcc cagttcgtta acatttttgc gttttgatag
    14041 cgcaaccttc aggaaaaatt atggagctgc tctgccctgc cggaaatctc ccggcgctta
    14101 aggcggccat cgaaaacggc gcagatgctg tttatatcgg gctaaaagat gataccaatg
    14161 cccgtcactt cgccggcctt aactttaccg agaaaaaatt gcaggaagcg gtgagttttg
    14221 tccatcaaca tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat ccggacggtt
    14281 acgcccgttg gcagcgtgcc gtggatatgg cggcgcagct gggtgccgac gcgctgatcc
    14341 tcgccgacct cgccatgcta gagtacgccg ccgagcgtta cccgcatatt gagcgtcatg
    14401 tgtcagtgca ggcttcggcg accaatgaag aggcgattaa cttttatcat cgccattttg
    14461 acgttgctcg cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa caactggcac
    14521 gggtcacacc tgtaccactg gaagtctttg ctttcggcag cctgtgcatt atgtcggaag
    14581 gtcgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc caacaccgta ggcgcgtgtt
    14641 ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg
    14701 aagtgctgat cgaccgttat caggacggcg aaaacgcagg ttatccgacg ctatgtaaag
    14761 ggcgttatct ggtggacggc gagcgctatc acgcgctgga agaaccaacc agtctcaata
    14821 ccctggaact gctgccggag ttaatggcgg cgaatattgc ttcggtgaaa attgaaggcc
    14881 gccagcgtag cccggcgtat gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc
    14941 gttgtaaggc cgatccgcaa aacttcatac cgcaaagcgc gtggatggag acgctcgggt
    15001 cgatgtccga aggcacgcag accacccttg gcgcgtatca ccgtaaatgg cagtgagaag
    15061 agcaatgaaa tattccttag ggccagtgct gtggtactgg ccaaaagaga cgctggaaga
    15121 tttttatcag caggccgcca ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg
    15181 cagcaagcgc cgggcaacca aagttggcga ctggctggag atggcaaaat cgctcgccgg
    15241 aagtggcaag cagattgtgc tctccacgct ggcgctggtg caggcatcct ctgaactggg
    15301 cgaactgaaa cgctatgttg agaacggtga gtttctgatt gaagccagcg atctcggcgt
    15361 ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta
    15421 caacgcggtg acgctgaaaa tattgctcaa acagggcatg atgcgctggt gtatgccggt
    15481 ggagctttcc cgcgactggc tggtgaatct gcttaatcag tgcgatgagc tgggcattcg
    15541 caaccagttt gaagtggaag tcctgagcta cggtcatcta ccgctggcct actccgcccg
    15601 ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct gctgcattaa
    15661 gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg
    15721 cattcagacc atgagcggct acgtttacaa cctcggtaac gagctggcat ccatgcaggg
    15781 gctggttgat gtggtccgcc tgtcaccgca gggtactgac actttcgcga tgctcgacgc
    15841 cttccgcgct aatgaaaatg gcgctgcgcc actaccgttg acggcaaaca gcgactgtaa
    15901 cggctactgg cggcggctgg ccgggctgga gctgcaagcc taagtaaata tgctcgtcat
    15961 acttcaagtt gcatgtgctg catctgcatt cgttcacccc agtcacttac ttatgtaagc
    16021 tcctggggat tcactctctt gtcgccttcc tgcaactcga attatttaga gcacagctca
    16081 ctttgttaac aactttaact actctttaat gcagtattaa agattaatcg gtaacaaagt
    16141 gagctgttat gactgataaa accattgcgt tttcgctact cgatctggcc cccattcccg
    16201 aaggttcttc agcgcgagaa gcattctccc actctctcga tctcgcccgt ctggctgaaa
    16261 agcgcggcta tcatcgctac tggctggcag aacaccacaa tatgactggc attgccagtg
    16321 ctgccacgtc ggtattgatt ggctatctgg cggcgaatac cacacgctgc atctggggtc
    16381 tggcggcgtg atgttgccta accactcacc gttggtcatt gccgaacagt tcggcacgct
    16441 caatacactc tatccggggc gaatcgattt ggggctgggt cgtgcgccag gtagtgacca
    16501 acggacaatg atggcgctgc gtcgccatat gagcggcgat attgataatt tcccccgcga
    16561 tgtcgcggag ctggtggact ggtttgacgc ccgcgatccc aatccgcatg tgcgcccggt
    16621 accaggctat ggcgagaaaa tccccgtgtg gttgttaggc tccagccttt acagcgcgca
    16681 actggcggcg cagcttggtc tgccgttcgc gtttgcctca cacttcgcgc cggatatgtt
    16741 gttccaggcg ctgcatcttt atcgcagcaa cttcaaaccg tcggcacgac tggaaaaacc
    16801 atacgcgatg gtgtgcatca atattatcgc cgccgatagc aaccgcgacg ctgagtttct
    16861 gtttacctca atgcagcaag cctttgtgaa gttgcgccgt ggcgaaaccg ggcaactgcc
    16921 gccaccgatt caaaatatgg atcagttctg gtcgccgtcc gaacagtatg gtgtgcagca
    16981 ggcgctgagt atgtcgttgg taggtgataa agcgaaagtg cgtcatggct tgcagtcgat
    17041 cctgcgcgaa accgacgccg atgagattat ggtcaacggg cagattttcg accaccaggc
    17101 gcggctgcat tcgtttgagc tggcgatgga tgttaaggaa gagttgttgg gatagtgtgt
    17161 cttaacgcgg gaagccttat ccgagctggc aacgctgtcc
//
LOCUS       DAZFRJ010000090        17153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000090 DAZFRJ010000000
VERSION     DAZFRJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.090"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..323
                     /gene="ybiJ"
                     /locus_tag="ACHKUN_002733"
     CDS             63..323
                     /gene="ybiJ"
                     /locus_tag="ACHKUN_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO6660965.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(555..1037)
                     /gene="ybiA"
                     /locus_tag="ACHKUN_002734"
     CDS             complement(555..1037)
                     /gene="ybiA"
                     /locus_tag="ACHKUN_002734"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO6660966.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            1157..3307
                     /gene="dinG"
                     /locus_tag="ACHKUN_002735"
     CDS             1157..3307
                     /gene="dinG"
                     /locus_tag="ACHKUN_002735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO6660967.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3335..4297
                     /gene="ybiB"
                     /locus_tag="ACHKUN_002736"
     CDS             3335..4297
                     /gene="ybiB"
                     /locus_tag="ACHKUN_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO6660968.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNTAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4438..5523
                     /gene="hcxB"
                     /locus_tag="ACHKUN_002737"
     CDS             4438..5523
                     /gene="hcxB"
                     /locus_tag="ACHKUN_002737"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO6660969.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5590..5649
                     /gene="ybiE"
                     /locus_tag="ACHKUN_002738"
     CDS             5590..5649
                     /gene="ybiE"
                     /locus_tag="ACHKUN_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO6660970.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5754..7118)
                     /gene="rhlE"
                     /locus_tag="ACHKUN_002739"
     CDS             complement(5754..7118)
                     /gene="rhlE"
                     /locus_tag="ACHKUN_002739"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO6660971.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            7347..8018
                     /gene="cecR"
                     /locus_tag="ACHKUN_002740"
     CDS             7347..8018
                     /gene="cecR"
                     /locus_tag="ACHKUN_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO6660972.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            8021..9016
                     /gene="hlyD"
                     /locus_tag="ACHKUN_002741"
     CDS             8021..9016
                     /gene="hlyD"
                     /locus_tag="ACHKUN_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO6660973.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            9009..10745
                     /gene="ybhF"
                     /locus_tag="ACHKUN_002742"
     CDS             9009..10745
                     /gene="ybhF"
                     /locus_tag="ACHKUN_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6660974.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10738..11871
                     /gene="ybhS"
                     /locus_tag="ACHKUN_002743"
     CDS             10738..11871
                     /gene="ybhS"
                     /locus_tag="ACHKUN_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6660975.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11882..12988
                     /gene="ybhR"
                     /locus_tag="ACHKUN_002744"
     CDS             11882..12988
                     /gene="ybhR"
                     /locus_tag="ACHKUN_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6660976.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12950..13360)
                     /gene="ybhQ"
                     /locus_tag="ACHKUN_002745"
     CDS             complement(12950..13360)
                     /gene="ybhQ"
                     /locus_tag="ACHKUN_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO6660977.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13493..14254
                     /gene="ybhP"
                     /locus_tag="ACHKUN_002746"
     CDS             13493..14254
                     /gene="ybhP"
                     /locus_tag="ACHKUN_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6660978.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            14251..15492
                     /gene="clsB"
                     /locus_tag="ACHKUN_002747"
     CDS             14251..15492
                     /gene="clsB"
                     /locus_tag="ACHKUN_002747"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO6660979.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15492..16448
                     /gene="ybhN"
                     /locus_tag="ACHKUN_002748"
     CDS             15492..16448
                     /gene="ybhN"
                     /locus_tag="ACHKUN_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO6660980.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16484..17143)
                     /gene="ybhM"
                     /locus_tag="ACHKUN_002749"
                     /pseudo
     CDS             complement(16484..17143)
                     /gene="ybhM"
                     /locus_tag="ACHKUN_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
BASE COUNT         4006 a         4483 c         4694 g         3970 t
ORIGIN      
        1 gatactcatc acatcgacga aacaacgtca cttatacaaa aatcacctgc gagagattaa
       61 ttatgaaaac tatcaatact gttgttgctg ctatggctct ttcaactctg tcatttggcg
      121 tgtttgccgc ggaaccggta acggcatccc aggcacagaa catgaataaa atcggcgtgg
      181 tttctgccga tggcgcatcc accctcgatg ccctggaagc gaaactggct gagaaagccg
      241 cggcagccgg tgccagtgga tacagcatca cttccgccac caacaacaat aaattaagcg
      301 gtactgcggt aatttataag taattcgtag gacggataag gcgtttacgc cgcatccggc
      361 aacagatgcc tgatgcgacg ctaccgcgtc ttatcagtat ctacataatc tctgcaatct
      421 attgaatttg catgtttttg taggccggat aaggcgttta cgccgcatcc ggcatttcac
      481 aacaagcact tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt taccgcccat
      541 aagccaccga gacgttactt ctctatagcc aattgttcgc gcaactccat taaaaggtag
      601 cccagtcgat tcttgccctt accattacca ccgtctcccc agtaagcatc gttttccgta
      661 tgctcaacca gttttgcggg cgcggttgcc agcaagagcg cacgcagttc tggatgctgt
      721 tcgaatttag cgcgaagtgc ttttcgcatc acctgttctt tgaccgactc ccagttttta
      781 cgcagaggct tagaacgatc gcgccccatg cgtgcggcga ccatagggga agaaacccga
      841 cggatctctt cgcggtattt ttcatcaagg aatttttgtg cctgaaaata gtgttctgag
      901 gtgggccagg ttttcccgtc aaccttgatg ggccaggcgg caaagttaga aaaatcaccg
      961 tagtcatcgc tggtgctgta gaaatttatg atggtgtctt gcatgacgtg ttggatcctt
     1021 tgtgctcgaa cgggcattaa accgcattat gttggtggtt attgcgagcc gctttccaga
     1081 aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct gtttatacag
     1141 tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg ccgcctggta
     1201 taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc ggcagatgat
     1261 tgcggacgtc gccaaaacgc tggccggaga agaagggcga catctggcga ttgaagcccc
     1321 caccggcgtt gggaaaacgc tctcctattt gatccccggc atcgccattg cccgcgaaga
     1381 gcaaaaaacg ctggtggtga gtaccgccaa cgtggcattg caggatcaga tttacagcaa
     1441 agatttaccg ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg cttttgggcg
     1501 tgggcgctac gtttgcccgc gtaatctgac ggcgctcgcc agcactgagc ccacgcaaca
     1561 ggatctgctg gcgtttcttg acgacgaact gacgccaaat aatcaggaag agcaaaaacg
     1621 ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg gatggtctgc gcgatcatac
     1681 tgatatcgct atagatgacg atctctggcg tcgtttaagt accgacaaag ccagctgcct
     1741 caaccgcaac tgttactact atcgtgaatg cccgtttttt gttgctcgtc gggagattca
     1801 ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg aaagcgaagc
     1861 cgtactacct gacccgaaaa atctgctgct ggtgctggac gaaggtcatc acctgccaga
     1921 tgtggcccgg gatgcgctgg agatgagtgc cgaaatcacc gcgccctggt atcgactaca
     1981 gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc cgaagaccat
     2041 cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt atgagcttat
     2101 cgcctcgtta aacaacattc tcaatctcta catgcctgcc gggcaggagg cagagcaccg
     2161 ttttgcgatg ggcgaactgc ctgatgaagt gctggagatc tgccagcggc tggcaaaact
     2221 caccgagatg ctgcgtggtc tggcggagtt atttcttaac gatttaagtg agaaaaccgg
     2281 cagccacgat attgtgcgtc tgcatcggtt gattttgcag atgaaccgcg cgttggggat
     2341 gttcgaggcg caaagcaaac tctggcggct ggcgtctctg gcgcaatctt ccggtgcgcc
     2401 ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctgcatctct ggtttcactg
     2461 cgtgggaata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta ttccgcacat
     2521 tattgtcacc tccgcaacct tgcgttcgct gaacagtttt tcgcgtttgc aggagatgag
     2581 tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct ttaaccactg
     2641 cgaacagggc aaaattgtta ttccccggat gcgctttgag ccttccatcg acaacgaaga
     2701 gcagcatatt gccgaaatgg cggccttttt ccgtaagcag gtggagagca aaaaacatct
     2761 cggtatgttg gtactgtttg ccagcggacg ggcgatgcag cgctttctcg actatgtgac
     2821 ggatttacgc ctgatgctgc tggtgcaggg cgatcagccg cgttatcgct tagttgaact
     2881 gcaccgcaaa cgcgttgcca acggtgagcg tagtgtgctg gtgggcttac agtcatttgc
     2941 cgaagggctt gatttgaaag gtgatctgct cagccaggtg catatccaca aaatcgcttt
     3001 tccgcccatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa gcctcaaccg
     3061 ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc agcaggttgg
     3121 gcgactgatt cgaagccacg gttgctgggg cgaagtggtt atctacgaca aacgcttgct
     3181 gaccaaaaat tatggcaagc gactactgga tgcattaccg gtatttccga tagagcaacc
     3241 ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac gccgtcggcg
     3301 gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa tcattaaaga
     3361 gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc gcggtctgta
     3421 tgcccatatg ctcaatggtg aagtccctga ccttgagttg ggcggcgtat tgattgcgct
     3481 gcgtatcaaa ggggaagggg aagcagagat gctcggcttt tacgaagcca tgcaaaatca
     3541 caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg attgtcatcc ccagttacaa
     3601 cggcgcgcgt aaacaggcca acctgacgcc gttgttggcg attctcctgc acaaactcgg
     3661 ttttcctgtg gtggttcacg gggttagcga agatccaacc cgcgtgctga ctgaaaccat
     3721 ttttgaattg atgggtatta cgccaacgtt gcacggcgga caggcgcagg cgaagctcga
     3781 cgaacatcaa ccggtgttta tgccagtcgg cgcgttttgc ccgccgctgg aaaaacaact
     3841 ggcgatgcgc tggcggatgg gcgtgcgtaa cactgcacat accctggcga aactggcaac
     3901 gccatttgcc gaaggtgagg cgctgcgtct ttccagcgtt tcgcatccgg aatacattgg
     3961 acgcgtcgcg aagttcttta gcgatatcgg cgggcgggcg ctactaatgc atggtactga
     4021 aggtgaagtg tatgctaatc cgcagcgctg cccgcagatc aatctcattg accgtgaagg
     4081 gatgcgggtg ctgtatgaaa aacaggacac tgctggtagc gagttactgc cacaagcaaa
     4141 agatccggaa accacggcgc agtggattga gcgttgcctt gctggcagcg aaccgattcc
     4201 cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag cggcaactat
     4261 cagcgacggc ctggcgcgcg ttaatcaggc attttaattc ttttctcagc cggatgagcc
     4321 atgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat tgaccttccc
     4381 acgcccggcg tgcagcataa aaatacaaca aacacataac ataaacagga gttaaccatg
     4441 gaaagtggtc atcgctttga tgctcagacg ctgcacagtt ttattcaggc tgtatttcgt
     4501 cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat cgcggcaaac
     4561 ctggcagggc atgattcaca tggtattggc atgatcccaa gctatgtacg ctcctggagt
     4621 caggggcacc tgcaaattaa ccatcatgcc aaaaccgtta aagagtcggg ggcggcggtc
     4681 acgctcgatg gcgatcgcgc atttggtcag gtcgcggcac atgaagcgat ggcgctgggg
     4741 attgagaaag cgcataagca cggtattgcc gccgtggcgc tacataactc gcatcatatc
     4801 ggccgtatcg gttactgggc ggagcagtgt gcagcggcgg ggtttgtctc tatccacttt
     4861 gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag ccgctttggc
     4921 accaatccgt tctgtgtggt tttccctcgt aaagataatt tcccgctgtt gcttgattac
     4981 gccaccagcg ccattgcatt tggtaaaact cgcgtcgcct ggcataaagg cgtccccgtg
     5041 ccgccaggtt gcctgattga cgttaacggc gtgccgacga ccaatccggc ggtaatgcag
     5101 gagtcgccgt tgggggcgct gttgaccttt gccgaacata aaggctacgc ccttgcagcg
     5161 atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa cgacgcatca ggaaacgtta
     5221 caaaccagtc ccgatgccat tcttaactgc atgaccacta tcatcatcaa cccggaactc
     5281 ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt gaaagcctcg
     5341 ccgcatgatg atgataagcc gattttgcta ccgggcgagt gggaagtgaa cacgcgtcgc
     5401 gaacggcaga agcaggggat tccactggat gcgggaagct ggcaggccat ttgtgatgca
     5461 gcgcggcaga ttggtatgcc ggaagagacg ttgcaggctt tctgtcagca gttagccagc
     5521 taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga tcaatgaggg
     5581 ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg acgtagcgta
     5641 aacgcctgat ccgtccaacg gttcggattt tgtagatact gataagcgta gcgcatcagg
     5701 cagttttgcg tttgttcatc agcctgatgc cgggcatagc ccggcataaa agattactgc
     5761 gcagcggtag gtttacgcgg acggcggcga cgttgttgtt cgcctgccgg tttggcatcg
     5821 ccgaggcggc gagacggttt ttctgcaggt ttcgcgcttg cagattttgc gccaccttcc
     5881 ccacggcgtg gttgctgttg accgcgacca ccaccttgcc cacgaccgcc gtcgccacgt
     5941 tgctggcgac cgttctggat cggttcggct ttgattgacg ggtccggctc atagcccgga
     6001 atcgcaatgc gcgggatctc ttttttcagc agtttttcga tatcacgcag cagtttgtgt
     6061 tcatcaacac acaccagcga caacgcttca ccggtagcag ccgcacgacc ggtacgcccg
     6121 atacggtgga cataatcttc cggtacgttt ggcagttcat agttgacgac gtgcggcaac
     6181 tcttcgatat ccagaccgcg cgcagcgata tcggtcgcca ccagtacgcg aatgtcaccc
     6241 gatttaaaat cagccagcgc acgggtacgc gcaccttgcg atttattccc gtggatcgcc
     6301 gcactacgga tgccatcttt attgagctgc tcagccagat ggttagcgcc gtgtttggta
     6361 cgggtaaaca ccagcacctg ctgccagttc cctttaccaa tcatgtgcga cagcaattcg
     6421 cgtttgcgtt tcttatcaac aaagtgaacg tgctgggtca cctgatcaga cgcggtattg
     6481 cggcgtgcca cttcgatttc cagcgggttg tgcaacagtt tttccgccag ggctttaata
     6541 tcgtcagaga aggtcgcaga gaacagcagg ttctggcgct tcgctggcag ttttgttaac
     6601 acgcgacgga tatcgtggat aaagcccatg tcgagcatgc ggtcagcttc atcgaggacg
     6661 aggatttcaa cctgatccag cttcactgca ttttgatgtt ccaggtccag caaacgtccc
     6721 ggtgttgcca ccagaacatc aacgccgccg cgcagcttca tcatttgcgg gttaatgctg
     6781 acaccgccaa acaccaccag cgaacgaatg ttcaaatatt tgctgtaatc acggacgttt
     6841 tcgccaatct gcgcagccag ttcacgggtc ggggtaagaa tgagcgcacg taccggacga
     6901 cgccctttgg cgtgcggctg gcgagtgatc aggtgttgca acagcggcag cgtaaagccc
     6961 gctgttttac cggtaccggt ctgggcgcta gccatcaggt cgcggccttc cagcaccgca
     7021 gggatcgcct gctgctgaat aggggtgggt tcacggtaac cctgctcggc aacggcgcgc
     7081 aggatatcag ggcttaaacc caaagaatcg aaagacataa ctactccgaa ccgccccgac
     7141 cgttaccggt gtagttttca gggagatact gaaaaggaaa atgacaaaaa ccacaatgtc
     7201 atgagggagc ggagtgtagc agtttttgtg acgcagcgca taaattatcc ctgtgactgg
     7261 cgtagctaaa attcttagcc atactggaca actcccatat ggagtcataa tcttaatcaa
     7321 tcatttgatt aagaatggac tgtgcgatga ataatcctgc catgacaatc aagggtgaac
     7381 aggcgaaaaa acagctgatt gctgccgcac tggcgcagtt tggtgaatat ggaatgaacg
     7441 ccaccacccg cgagatagcc gcccaggccg ggcagaatat cgctgccatc acctactact
     7501 tcggttcgaa agaagatttg tacctcgcct gcgcccagtg gattgccgat tttattggcg
     7561 agcagttccg tccgcacgcc gaagaggccg aacgcttgtt cgcacaacca cagcctgatc
     7621 gcgcagccat ccgtgaactg atccttcgcg cctgcaggaa catgattaag ctgctcaccc
     7681 aggatgacac cgtcaacctc agcaagttta tctcccgtga gcagctctct cccacggcag
     7741 cctatcacct ggtgcacgaa caggtgatta gcccgctaca cagccacctg acacgcctga
     7801 ttgccgcctg gaccggctgc gacgccaacg atacccgcat gatcctccat actcatgcgt
     7861 tgattggtga gattctggcg ttccgtcttg gcaaagaaac aattctgtta cgtaccggct
     7921 ggaccgcgtt cgatgaagaa aagaccgaac tgatcaacca gacggtgacc tgtcatatcg
     7981 acctgatttt gcaaggatta tcgcaaagga gtttgtagtg atgaaaaaac ctgtcgtgat
     8041 cggactggcg gtagtggtac ttgccgccgt ggttgccgga ggctactggt ggtatcaaag
     8101 ccgccaggat aacggcctga cgctgtatgg caacgtggat attcgtacgg tgaatcttag
     8161 tttccgtgtt ggggggcgcg ttgaatcgct ggcggtggac gaaggtgatg ctatcaaagc
     8221 gggccaggtg ctgggcgaac tggatcacaa gccgtatgag attgccctga tgcaggcgaa
     8281 agcgggtgtt tcggtggcac aggcgcagta tgacctgatg cttgccgggt atcgcgatga
     8341 agaaatcgct caggccgccg cagcggtgaa acaggcgcaa gccgcctatg actatgcgca
     8401 gaacttctat aaccgccagc aagggttgtg gaaaagccgc accatttcgg caaacgacct
     8461 ggaaaatgcc cgctcctcgc gcgaccaggc gcaggcaacg ctgaaatcag cacaggataa
     8521 attgcgtcag taccgttccg gtaaccgtga acaggacatc gcccaggcga aagccagcct
     8581 cgaacaggcg caggcgcaac tggcgcaggc ggagttgaat ttgcaggact caacgttgat
     8641 agccccgtct gatggcacgc tgttaacgcg cgcggtggag ccaggcacgg tcctcaatga
     8701 aggtggcacg gtgtttaccg tttcactaac gcgtccggtg tgggtgcgcg cttatgttga
     8761 tgaacggaat cttgaccagg cccagccggg gcgcaaagtg ctgctttata ccgatggtcg
     8821 cccggacaag ccgtatcacg ggcagattgg tttcgtttcg ccgactgctg aatttacccc
     8881 gaaaaccgtc gaaacaccgg atctgcgtac cgacctcgtc tatcgcctgc gtattgtggt
     8941 gaccgacgcc gatgatgcgt tacgccaggg aatgccggtg acggtgcaat tcggtaacga
     9001 ggcaggacat gaatgatgcc gttatcacgc tgaacggcct ggaaaaacgc tttccgggca
     9061 tggacaagcc cgccgtcgcg ccgctcgatt gtaccattca cgccggttat gtgacggggc
     9121 tggtggggcc ggatggtgca ggtaaaacca cgctaatgcg gatgctggcg ggattactga
     9181 aacccgacag cggcaatgcc acggtgattg gctttgatcc aatcaaaaac gacggcgcgc
     9241 tgcacgccgt gctcggctat atgccgcaga aatttggtct gtatgaagat ctcacggtga
     9301 tggagaacct caatctgtac gcggatttgc gcagcgtcac cggcgaggca cgtaagcaaa
     9361 cttttgctcg cctgctggag tttacgtctc ttgggccgtt taccggacgc ctggcgggca
     9421 agctctccgg tgggatgaaa caaaaactcg gtctggcctg taccctggtg ggcgaaccga
     9481 aagtgttgct gctcgatgaa cccggcgtcg gcgttgaccc tatctcacgg cgcgaactgt
     9541 ggcagatggt gcatgagctg gcaggcgaag ggatgttaat cctctggagt acctcgtatc
     9601 tcgacgaagc cgagcagtgc cgtgacgtgt tactgatgaa cgaaggcgag ttgctgtatc
     9661 agggggaacc aaaagccctg acacaaacca tggccggacg cagttttctg atgaccagtc
     9721 cacacgaggg caaccgcaaa ctattgcaac gcgccttgaa actgccacag gtcagcgacg
     9781 gcatgattca ggggaaatcg gtacgtctga tcctcaaaaa agaggccaca ccagacgata
     9841 ttcgccatgc cgacgggatg ccggaaatca acatcaacga aactacgccg cgttttgaag
     9901 atgcatttat tgatttgctg ggtggtgccg gaacctcaga atcgccgctg ggcgcaatat
     9961 tacatacggt agaaggcaca cccggcgaga cggtgatcga agcgaaagaa ctgaccaaga
    10021 aatttggtga ttttgccgcc accgatcacg tcaactttgc cgttaaacgt ggggagattt
    10081 ttggtttgct ggggccaaac ggcgcgggta aatcgaccac ctttaagatg atgtgcggtt
    10141 tgctggtgcc gacttccggc caggcgctgg tgctggggat ggatctgaaa gagagttctg
    10201 gtaaagcgcg ccagcatctc ggctatatgg cgcaaaagtt ttcgctctac ggtaacctga
    10261 cggtcgaaca gaatttacgc tttttctctg gtgtgtatgg cttacgcggt cgggcgcaga
    10321 acgaaaaaat ctcccgtatg agcgaggcgt tcggcctgaa aagtatcgcc tcccacgcca
    10381 ccgatgaact gccattaggt tttaaacagc ggctggcact ggcctgctcg ctgatgcatg
    10441 aaccggacat tctgtttctc gacgaaccga cgtccggcgt tgatccgatt actcgccgcg
    10501 aattctggct acatatcaac agcatggtag agaaaggcgt cacggtgatg gtcaccaccc
    10561 actttatgga tgaagcagaa tattgcgacc gcatcggcct ggtgtaccgt gggaaattaa
    10621 ttgccagcgg cacgccggat gatttgaaag cgcagtcggc caacgacgag caacccgatc
    10681 ccaccatgga gcaagccttt attcagttga tccacgactg ggataaggag catagcaatg
    10741 agtaacccga tcctgtcctg gcgtcgcgta cgggcgctgt gcgttaaaga gacgcggcag
    10801 atcgttcgcg atccgagtag ctggctgatt gcggtagtga tcccgctgct actgctgttt
    10861 atttttggtt acggcattaa cctcgactcc agcaagctgc gggtcgggat tttactggaa
    10921 cagcgtagcg aagcggcgct ggatttcacc cacaccatga ccggttcgcc ctacatcgac
    10981 gccaccatca gcgataaccg tcaggaactg atcgccaaaa tgcaggcggg gaaaattcgc
    11041 ggtctggtgg ttattccggt ggattttgcg gaacagatgg agcgcgccaa cgccaccgca
    11101 ccgattcagg tgatcaccga cggcagtgag ccgaataccg ctaactttgt acaggggtat
    11161 gtcgaaggga tctggcagat ctggcaaatg cagcgagcgg aggacaacgg gcagactttt
    11221 gaaccgctta ttgatgtaca aacccgctac tggtttaacc cggcggcgat tagccagcac
    11281 ttcattatcc ccggtgcggt gaccattatc atgacggtca tcggcgcgat tctcacctcg
    11341 ctggtggtgg cgcgagaatg ggaacgcggc accatggagg ctctgctctc tacggagatt
    11401 acccgcacgg aactgctgct gtgtaagctg atcccttatt actttctcgg gatgctggcg
    11461 atgttgctgt gtatgctggt gtcagtgttt attctcggcg tgccgtatcg cgggtcgctg
    11521 ctgattctgt tttttatctc cagcctgttt ttactcagta ccctggggat ggggctgctg
    11581 atttccacga ttacccgcaa ccagttcaat gccgctcagg tcgccctgaa cgccgctttt
    11641 ctgccgtcga ttatgctttc cggctttatt tttcagatcg acagtatgcc cgcggtgatc
    11701 cgcgcggtga cgtacattat tcccgctcgt tatttcgtca gcaccctgca aagcctgttc
    11761 ctcgccggga atattccagt ggtgctggtg gtaaacgtgc tgtttttgat cgcttcggcg
    11821 gtgatgttta tcggcctgac gtggctgaaa accaaacgtc ggctggatta gggagaagag
    11881 catgtttcat cgcttatgga cgttaatccg caaagagttg cagtcgctgc tgcgcgaacc
    11941 gcaaacccgc gcgattctga ttttacctgt gcttattcag gtgattctgt tcccgttcgc
    12001 cgccacgctg gaagtgacta acgccaccat cgccatctac gatgaagata acggcgagca
    12061 ttcggtggag ctgacccaac gttttgcccg cgccagcgcc tttactcatg tgctgctgct
    12121 gaaaagccca caggagatcc gcccaaccat cgacacacaa aaggcgttac tactggtgcg
    12181 tttcccggct gacttctcgc gcaaactgga taccttccag accgcgccgt tgcagttgat
    12241 cctcgacggg cgtaactcca acagtgcgca aattgccgcc aactacctgc aacagatcgt
    12301 caaaaattat cagcaggagc tgctggaagg aaaaccgaaa cctaacaaca gcgagctggt
    12361 ggtacgcaac tggtataacc cgaatcttga ctacaaatgg tttgtggtgc cgtcgctgat
    12421 cgccatgatc accactatcg gcgtaatgat cgtcacttca ctttccgtcg cccgcgaacg
    12481 tgaacaaggt acgctcgatc agctactggt ttcgccgctc accacctggc agatcttcat
    12541 cggcaaagcc gtaccggcgt taattgtcgc caccttccag gccaccattg tgctggcgat
    12601 tggtatctgg gcgtatcaaa tccccttcgc cggatcgctg gcgctgttct actttacgat
    12661 ggtgatttac ggtttatcgc tggtgggatt cggtctgttg atttcatcac tctgctcgac
    12721 acaacagcag gcgtttatcg gcgtgtttgt ttttatgatg cccgccattc tcctttccgg
    12781 ttacgtttct ccggtggaaa acatgccagt atggctgcaa aacctgacgt ggattaaccc
    12841 tattcgccac tttacggaca ttaccaagca gatttatttg aaggatgcga gtctggatat
    12901 tgtgtggaat agtttgtggc cgctactggt gataacggcc acgacagggt cagcggcgta
    12961 cgcgatgttt agacgtaagg tgatgtaact tcttatcttt cgccagcaaa gacactaccg
    13021 ccgggccggc aaggattgcc agccctgcaa ttgccagcaa aaagttgttt tccagtacac
    13081 gggacaacag ccacagcaca atcagcgctg cgccaaaata ccaggtcgtg gtcagctctt
    13141 ccagcacgtc tgccacctca cgccagcgtt gctcggggtg gcgctgaaac accaacatca
    13201 tcactaccgt gacacaatac agtgcgcata atcccacgcc gacgtgaacc agagtcttac
    13261 tcatccagcc caccaccagc agcgccacta ccagtaaatg caacataatc tgccagcaac
    13321 ttagaccagt tgcgacacga acacgttgtt gccacttcat ggcttctctc ctgaagaatt
    13381 tatctctact tactcagagt accgaacttt acggaaaatt ccgtaagacc gcaccttttt
    13441 gtgacgccga ctacactatt tcaatgagat aagaggcaaa gacaggaaaa ttatgcccga
    13501 tcaaacacaa caattttcgt tcaaggtgct caccatcaac attcacaaag gttttaccgc
    13561 gtttaaccga cgcttcattt tgccggaact tcgcgacgcg gtgcgtaccg tcagcgccga
    13621 tattgtttgc ctgcaggaag tgatgggcgc gcacgaagtt catccgctgc atgtggaaaa
    13681 ctggcccgat acctcgcact acgagtttct cgccgacacg atgtggagcg attttgccta
    13741 cggtcgcaat gccgtatacc cgaaagggca tcacggcaac gccgtactgt cgcgttatcc
    13801 cattgaacat tatgagaatc gcgatgtttc ggtcgatggt gcggaaaagc gcggcgtgct
    13861 ctactgccgc attgtgccac cgatgaccgg aaaagcgatt catgtgatgt gcgtacatct
    13921 gggcctgcgt gaggcgcacc gtcaggcgca gcttgcgatg ctcgccgaat gggtgaatga
    13981 gctaccggac ggcgaaccgg tattggtggc gggtgatttc aatgactggc ggcaaaaagc
    14041 taatcatccg ttaaaagtgc aggccggact ggatgagatt tttacccgcg cccacggacg
    14101 cccggcgcgc acgtttccgg tgcaatttcc tctactacga ctggacagga tctacgtcaa
    14161 aaatgccagc gccagcgcgc caaccgcgtt gccgctgcgg acatggcgac acctttctga
    14221 tcatgcccct ttaagtgcgg agattcattt atgaaatgta gctggcgcga aggcaataag
    14281 atccagttgc tggaaaacgg cgagcaatat tatcccgcgg tgtttaaggc gattggcgag
    14341 gcacaagaac gcatcattct tgaaacgttt atctggtttg aggatgacgt cggcaaacag
    14401 ctgcatgcgg cactactggc agcagcgcaa cgcggggtta aagcggaagt cttgctggat
    14461 ggctacggtt cgccggatct cagcgatgag tttgtcaatg aactgacggc agctggcgtg
    14521 gtatttcgct actacgatcc ccgccctcgc ctttttggta tgcgcaccaa tgtgtttcgc
    14581 cggatgcatc gcaaaattgt ggtgatcgac gcgcgtatcg cctttattgg cgggctgaat
    14641 tactccgccg agcatatgtc cagctacggt ccagaggcta aacaggatta cgcggtacgc
    14701 cttgaagggc cgattgtcga agatattctc cagtttgagc tggaaaacct gcctggacag
    14761 agcgcggcac gacgctggtg gcgacgtcat cacaaagcgg aagagaaccg ccagccggga
    14821 gaagcgcagg tattgctggt ctggcgcgat aacgaagaac atcgcgatga tattgaacgt
    14881 cactatctga aaatgctcac tcaggcgcgg cgagaagtca ttatcgccaa cgcctacttc
    14941 ttccccggct atcgattttt acacgccttg cgtaaagcgg cacggcgcgg ggtgcggatc
    15001 aaactgatca ttcagggcga accggatatg ccgattgtca gagtcggtgc gcgcttgctg
    15061 tataactatc tggttaaagg cggcgttcag gtatttgagt accgccgccg cccgctccac
    15121 ggcaaagtgg cattgatgga cgatcactgg gcgacagtag ggtccagtaa tctcgatccg
    15181 ctcagtttgt cactgaatct cgaagcaaat gtcatcatcc acgatcgtca ttttaaccag
    15241 acgctgcgcg ataatctgaa cggcattatt gccgcagatt gtcagcaggt cgatgagacc
    15301 atgctgccga aacgcacctg gtggaacctg accaaaagcg tgctggcgtt ccacttttta
    15361 cgccacttcc cggcgctggt tggctggctt ccggcacaca caccacgtct ggcgcaggtt
    15421 gatccgcccg cacaaccgac aatggaaacg caggatcggg tagaaactga aaacacgggg
    15481 gtaaaaccct gatgagtaaa tcacacccgc gctggcgctt agcaaagaag atcctcacct
    15541 ggctgttttt tatcgcggtg attgtgttgc tggtggtcta cgccaaaaaa gtggactggg
    15601 aagaggtctg gaaggtcatc cgcgactaca atcgcgttgc gctgcttagt gcggtcgggc
    15661 tggtggtcgt cagctatctg atttacggct gctatgacct gctcgcccgc ttttactgcg
    15721 gtcacaaact ggcgaagcgc caggtgatgc tggtgtcgtt tatctgctac gccttcaacc
    15781 tgacgctcag tacctgggtc ggcggcattg gtatgcgcta tcgtttgtac tcccgactag
    15841 ggttaccggg cagcactatt acgcggattt tctcgctcag tattaccacc aactggctgg
    15901 gctatatttt actggcaggg attatcttta ccgcaggcgt ggtggagttg ccggatcact
    15961 ggtatgtcga tcaaactacg ctgcgtattc tcggcattgg cttactgatg attatcgcgg
    16021 tttatttgtg gttttgcgct ttcgcgaagc accgccatat gaccatcaaa gggcaaaagc
    16081 tggtgctgcc ttcatggaaa ttcgccctcg ctcagatgct gatttccagc gttaactgga
    16141 tggtaatggg ggcgattatc tggctgttac ttggtcaaag cgtgaactat ttctttgtac
    16201 tgggcgtgtt actggttagt agtattgctg gcgtcatcgt gcatattccg gtggggatcg
    16261 gtgtgctgga agcggtgttt atcgcactac tggctgggga gcatacctcg aaagggagca
    16321 ttatcgccgc cctgctcgct tatcgcgtcc tgtattactt tatcccgctg ctgctggcgc
    16381 tgatctgtta cctgctactg gaaagccagg cgaagaagct acgggcgaaa aatgaagcgg
    16441 cgatgtgaag tttagttaag ttctttagta tctgcattta cgactaatga aaaaaacgcg
    16501 tatgcctttg ccagacaagc gttatagcta tcgcggcaag tatcccaaag aagccgataa
    16561 aatagtaata gagtgttaat gcacctgtaa cagcgcaacg aaccggactc cacaactctt
    16621 cactatggca tttccccgcc aattcaagga gcttatttcg tccatgcgag ataatgcctg
    16681 accataacac aatcattaag caacttatta cccaaacggg tcgttcactc attaatgcgg
    16741 cattcatgat gatcaccagg gccaaccctg tcaacgtcat cataatgata aaacggtgag
    16801 aaccaggatc aacattaaat aataaaccgg ccagcatact gattaaaaac atcgcgcccg
    16861 cagtgctgaa cacgacgata atggcaggta caggagctaa acttgcaacg acaatcgcca
    16921 ccaaagagag taaaaacaga tatattacgc ctgcgatgac ggcatttatc ttgttgatat
    16981 ccttacttaa gccatacata caaactaata ataaccctat ggcgacatac attgttggtg
    17041 aatcatattt ggttgccgta atttcgagat acttagctcc ctaggccagt cctgcggttg
    17101 ccaccaaacc taaagcggta atgagccata taccgtttaa tatcctggtc tcg
//
LOCUS       DAZFRJ010000091        17123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000091 DAZFRJ010000000
VERSION     DAZFRJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.091"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            107..1246
                     /gene="arnB"
                     /locus_tag="ACHKUN_002750"
     CDS             107..1246
                     /gene="arnB"
                     /locus_tag="ACHKUN_002750"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO6660981.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1250..2218
                     /gene="arnC"
                     /locus_tag="ACHKUN_002751"
     CDS             1250..2218
                     /gene="arnC"
                     /locus_tag="ACHKUN_002751"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO6660982.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2218..4200
                     /gene="arnA"
                     /locus_tag="ACHKUN_002752"
     CDS             2218..4200
                     /gene="arnA"
                     /locus_tag="ACHKUN_002752"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO6660983.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4197..5087
                     /gene="arnD"
                     /locus_tag="ACHKUN_002753"
     CDS             4197..5087
                     /gene="arnD"
                     /locus_tag="ACHKUN_002753"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO6660984.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5087..6739
                     /gene="arnT"
                     /locus_tag="ACHKUN_002754"
     CDS             5087..6739
                     /gene="arnT"
                     /locus_tag="ACHKUN_002754"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO6660985.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6736..7071
                     /gene="arnE"
                     /locus_tag="ACHKUN_002755"
     CDS             6736..7071
                     /gene="arnE"
                     /locus_tag="ACHKUN_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO6660986.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7071..7457
                     /gene="arnF"
                     /locus_tag="ACHKUN_002756"
     CDS             7071..7457
                     /gene="arnF"
                     /locus_tag="ACHKUN_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO6660987.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7451..7717)
                     /gene="pmrD"
                     /locus_tag="ACHKUN_002757"
     CDS             complement(7451..7717)
                     /gene="pmrD"
                     /locus_tag="ACHKUN_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO6660988.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7827..9182)
                     /gene="menE"
                     /locus_tag="ACHKUN_002758"
     CDS             complement(7827..9182)
                     /gene="menE"
                     /locus_tag="ACHKUN_002758"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO6660989.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9179..10141)
                     /gene="menC"
                     /locus_tag="ACHKUN_002759"
     CDS             complement(9179..10141)
                     /gene="menC"
                     /locus_tag="ACHKUN_002759"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO6660990.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETREEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10141..10998)
                     /gene="menB"
                     /locus_tag="ACHKUN_002760"
     CDS             complement(10141..10998)
                     /gene="menB"
                     /locus_tag="ACHKUN_002760"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO6660991.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11013..11771)
                     /gene="menH"
                     /locus_tag="ACHKUN_002761"
     CDS             complement(11013..11771)
                     /gene="menH"
                     /locus_tag="ACHKUN_002761"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO6660992.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11768..13438)
                     /gene="menD"
                     /locus_tag="ACHKUN_002762"
     CDS             complement(11768..13438)
                     /gene="menD"
                     /locus_tag="ACHKUN_002762"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO6660993.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13527..14822)
                     /gene="menF"
                     /locus_tag="ACHKUN_002763"
     CDS             complement(13527..14822)
                     /gene="menF"
                     /locus_tag="ACHKUN_002763"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO6660994.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14901..15206)
                     /gene="elaB"
                     /locus_tag="ACHKUN_002764"
     CDS             complement(14901..15206)
                     /gene="elaB"
                     /locus_tag="ACHKUN_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO6660995.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15261..15722)
                     /locus_tag="ACHKUN_002765"
     CDS             complement(15261..15722)
                     /locus_tag="ACHKUN_002765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6660996.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15787..16704
                     /gene="rbn"
                     /locus_tag="ACHKUN_002766"
     CDS             15787..16704
                     /gene="rbn"
                     /locus_tag="ACHKUN_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO6660997.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16892..>17123
                     /locus_tag="ACHKUN_002767"
     CDS             16892..>17123
                     /locus_tag="ACHKUN_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO6660998.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTN"
BASE COUNT         3985 a         4629 c         4358 g         4151 t
ORIGIN      
        1 aagttaatat tctatcctta aaatttcgct ccaaatggca aaatatacac aacactcttt
       61 atagcaaata taagtggaca ggtattcaat ggcggaagga aaagcaatgt cagaattttt
      121 gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag aggttctcga
      181 atccggttgg atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt
      241 gacgggaaat cagcatgcca tcgcggtcag ttcagccacc gccggaatgc atatcacgct
      301 aatggcgttg gaaattggca agggcgatga agtgattacg ccttccctga cctgggtttc
      361 aaccctcaat atgatttcct tgttgggtgc aacgccggta atggtggatg tcgaccgcga
      421 tacgccgatg gtcacgcctg aagcgatcga gtcagccata acgccacgca ctaaagccat
      481 cattccggtg cattatgccg gtgcgccagc agatattgac gccattcgcg ccattggcga
      541 acgttacggc atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg
      601 gcgacatatt ggcgcaaaag gtaccactat tttttcattt catgccatta aaaatattac
      661 ctgtgcggaa ggtggcctga ttgtaactga taatgaaaac cttgcccgcc agctacggat
      721 gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc
      781 gcaggctgaa gtcttaacac cgggctataa gtacaatctg accgatatta acgccgcaat
      841 tgccctgaca cagttagcaa aattagagca cctcaacacc cgtcggcgcg aaattgccca
      901 gcaatatcag caagcactgg cagctctccc ctttcagcca ttaagccttc ccgcctggcc
      961 gcacgttcac gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag
     1021 tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt tacatttccg
     1081 cgccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt taccgaatac
     1141 cgaatggaat agcgaacgca tctgttcttt gccgctgttc ccggatatga ctaccgccga
     1201 tgccgaccgt gtcatcacag cccttcagca actcgcagga caataagcca tgtttgaaat
     1261 ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg aaagcttacc
     1321 ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt atgagatcct
     1381 gctgattgat gacggcagta gcgataattc cgcgcatatg ctggtcgaag cctcacaagc
     1441 ggagaacagc catattgtgt ctattttgct taaccgcaat tacgggcaac attcagcgat
     1501 tatggcggga ttcagtcacg ttaccggcga cttaattatt acccttgatg ccgatctcca
     1561 gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt
     1621 agggactgta cgccagaacc gccaggacag ctggtttcgt aaaacagctt cgaagatgat
     1681 taaccggctt attcagcgca ccaccggcaa agcgatgggt gactacggtt gtatgctgcg
     1741 cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgta gcacctttat
     1801 cccgattctg gcgaatatct tcgcccgccg tgccattgaa attccagtac atcattccga
     1861 gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga tgtacgacct
     1921 ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcggca gcattattgc
     1981 gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct tcggaccaca
     2041 atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta ttggcgctca
     2101 gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg atgtccgcgc
     2161 ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa atgaataatg
     2221 aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc tctgctggct
     2281 gccggttacg aaattagcgc catttttacc cataccgata atcccggtga aaaagccttt
     2341 tatggttcgg tggctcgtct ggcagcggaa agaggcattc cggtttatgc gccggatgac
     2401 gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt cgccagatgt gattttctct
     2461 ttttattatc gccatcttat ttgcgacgaa attttgcagc tcgctcccgc aggtgcattt
     2521 aatctgcatg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg
     2581 gtcaacggtg aaacggaaac tggcgttaca ttgcaccgaa tggtgaaacg tgccgatgcc
     2641 ggggccattg tggcgcaact gcgcattgcc attgcgccag acgatatcgc tattacgctg
     2701 catcataaat tgtgccatgc cgcgcgccag ctactggaac agacattacc cgccattaaa
     2761 cacggtaata ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga
     2821 acgccggatg acagtttcct tgaatggcat aaaccggcat ccgtactgca caacatggta
     2881 cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca gaaattcacc
     2941 gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac agccggggag cgtgatttct
     3001 gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg
     3061 ggcgacggca ttactatgca gggctcgcaa ttagcgcaga cgctgggcct ggtgcaaggt
     3121 tcacgcttga atagccagcc tgcctgcacc gcccgacgcc gtacccgggt actcatcctc
     3181 ggggtgaatg gctttattgg caaccatctg acagaacgcc tgctgcgcga agatcattat
     3241 gaagtttacg gtctggatat tggcagtgat gcgataagcc gttttctgaa tcatccgcat
     3301 tttcactttg ttgaaggcga tatcagtatt cattccgaat ggattgagta tcacgtcaaa
     3361 aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc cgattgaata tacccgcaac
     3421 ccgctgcgcg tatttgaact cgattttgaa gagaatctgc gcattatccg ctactgcgtg
     3481 aagtaccgta agcgaatcat cttcccgtca acttcagaag tttatgggat gtgtagcgat
     3541 aaatacttcg atgaggacca ttctaattta atcgtcggcc cggtgaataa accacgctgg
     3601 atttattcgg tatcaaaaca attacttgat cgggtgatct gggcctatgg cgaaaaagag
     3661 ggtttacagt tcaccctctt ccgcccgttt aactggatgg gaccgcgact ggataacctt
     3721 aatgcggcac gaatcggcag ctcccgcgct attacgcaac tcattctcaa tctggtagaa
     3781 ggttcaccga ttaagctgat tgatggcgga aaacaaaaac gctgctttac tgatattcgc
     3841 gatggtatcg aggcgttata ccgcattatc gaaaatgcgg gaaatcgctg cgacggtgaa
     3901 attatcaaca ttggcaatcc tgagaacgaa gcgagcattg aggaactggg cgagatgctg
     3961 ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc gggctttcgc
     4021 gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg
     4081 agcatccgca atgcccaccg ctgcctggac tgggagccga aaattgatat gcaggaaacc
     4141 atcgacgaaa cgctggattt cttcctgcgc accgttgatc ttacggataa accatcatga
     4201 ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc
     4261 gtctgctgga aatcttgagt aagcataata ttcaggccag catttttttc agcgtcggcc
     4321 cggacaatat gggccgccat ctctggcgac tggtgaagcc acagtttttg tggaagatgc
     4381 tgcgctcaaa tgcggcatcg ctttatggct gggatatttt actggcaggt acggcctggc
     4441 caggtaaaga gattggtcat gccaatgccg atatcattcg tgaagcggct aaacatcacg
     4501 aagtcggcct gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt aactgggatc
     4561 ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac tctggaagag attatcggtc
     4621 aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa
     4681 aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg ttccgtccat
     4741 tgctcgaatc aggaaaccct ggcactgcgc aaattccggt gaccttaccc acctgggatg
     4801 aagtgattgg tcgggatgtg aaagcagaag attttaacgg ttggttactc aaccgcatcc
     4861 tgcgagataa aggcacgccg gtttatacca ttcatgcaga agttgaaggc tgcgcttatc
     4921 agcataattt tgtggatctc ctcaaacgcg cagctcagga aggagtgaca ttttgccctt
     4981 taagcgaact gttatcagag acgttgccgc tcggacaagt tgttcgcgga aatattgccg
     5041 gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg
     5101 ttaccttatc ggcctcttcg cgtttattgc ctgctattac ctgttaccga tcagcacgcg
     5161 tctgctctgg caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa tgctggcatc
     5221 cggcgactgg attgtgcccc atctgttagg gctacgttat ttcgaaaaac ccattgccgg
     5281 atactggatt aacagcattg ggcaatggct atttggcgcg aataactttg gtgtgcgggc
     5341 aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg acctggttta ctctgcgctt
     5401 atggcgcgat aaacgtctgg ctctactcgc cacagtaatt tatctctcat tgtttattgt
     5461 ctatgccatc ggcacttatg ccgtgctcga tccgtttatt gcattctggc tggtggcggg
     5521 aatgtgcagc ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt
     5581 tttactgctg ggaatcacct gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc
     5641 cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga aagatctctt
     5701 tatttacggc tggctggcgg ttatcagttg cgtactgacg gttctccctt ggggactggc
     5761 gatagcgcag cgggagcctg acttctggca ctattttttc tgggttgagc atattcaacg
     5821 ctttgcactg gatgatgccc aacatagagc tccgttctgg tactacgtgc cggtcatcat
     5881 tgccggtagc ctgccgtggc tgggattact ccccggtgca ctgtacacag gctggaaaaa
     5941 ccgcaagcat tccgcaaccg tctatttgtt gagctggacg ataatgccgc tgctgttttt
     6001 ctccgtcgct aaaggtaaat tgcccaccta tattctttcc tgctttgcat ctctggcaat
     6061 gctgatggcg cattacgctt tgctggcagc aaaaaataat cctctggcgc tgcggattaa
     6121 tggctggatt aacatcgctt ttggcgtcac tggcattatt gccacatttg tggtctcccc
     6181 gtggggacca atgaacacgc cggtgtggca aaccttcgag agctataaag tcttttgtgc
     6241 ctggtcgatt ttttcgctat gggcattttt cggctggtac accttaacaa acgtcgaaaa
     6301 gacctggcct tttgccgcgc tttgcccgct ggggctggcg ttgctggtag gattttcaat
     6361 tcctgacaga gttatggaag gaaaacatcc gcaatttttt gtcgagatga cacaagaatc
     6421 acttcagcca agccgctata ttcttactga tagcgtcggt gttgccgcag gtctggcatg
     6481 gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca ggtgagttga aatacggcct
     6541 taattatccg gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa actggcttaa
     6601 tcaacatcgt caggagggga ttattactct cgtgctttcg gttgaccgcg atgaagatat
     6661 caacagtctc gccattccgc ccgcagatgc catcgatcgt caggagcgtc tggtgctgat
     6721 tcagtatcgt cccaaatgat ctggctaaca ttagtctttg ccagcttgct tagcgttgcc
     6781 gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat
     6841 atcgtgctgt ggctgggact ggcgctggct tgtcttggtc ttgccatggt gctctggctg
     6901 ctggtcttgc agaacgtacc ggtaggcatt gcctacccga tgttaagtct gaattttgtc
     6961 tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt
     7021 ggggtggcgt tcattattgg tggcattgtg atcctcggga gtacggtgta atgggcctga
     7081 tatggggatt attcagcgtc attattgcct cagttgcgca attaagcctg ggttttgcgg
     7141 caagtcatct gccgccgatg acgcacttat gggattttat cgcggctctg ttagcttttg
     7201 gcctggatgc ccggatactg ttactcgggc tgctgggtta tctgctttcc gttttttgtt
     7261 ggtataaaac gctacataaa cttgcgctaa gcaaagccta tgccttgctc agtatgagtt
     7321 atgtgctggt atggattgct tcaatggtac ttcccggctg ggaagggact ttttcgctga
     7381 aagcactact gggagtagcc tgtattatga gcgggttgat gctgattttc ctgcccacga
     7441 caaaacaacg ttactgagtt ttccctgccg ctgtacactg ccgttcccac tcatcggggc
     7501 tataactgct tgccgagagg actttcaccg tatgcagctt ttcacgatta atacaataaa
     7561 gcgcattctg caaaggcgag agtaaatccc ccactttcac ggcgaagtca ctcttcactt
     7621 cggcgatcat ttttatcgcg ccgccagcat cgcagagcat aagtacatgt ctgttatctt
     7681 gtttattgca gcacgatttt ttgaccagcc attccattgc attatcctgt ttgctaagag
     7741 ttttcacatc aatttacaga ttaacagata aactctttat caacggcaat ggaatggcag
     7801 aaatgagaag tgaaacggag ggttaattat tgctgacgtt gcacccactc ttttagcgcc
     7861 tgacgtgaaa ttttaatacc gccgtttttc agctccggcg gtaaagtcag ccagcgcacc
     7921 ggttgttgaa aacgagccag cttatctttc acccattcgc caagatcaac ggtttgctgg
     7981 tcatactcca ccaccgctac cggccgatga ccaaactcct tgtcggcaac gggaacgata
     8041 aacacctgca aaaccgcagg atgtgcagca ataacacgct cgacttcttc cggctgaata
     8101 ccctctccgc cactgaagaa tagattgtct aaacgtccga caatggtcag cttgccatta
     8161 tgcatctcac cgcgatcgcg cgtagcgtac cagccttcgt cattaaccag tgaaaccagt
     8221 tgcccgttac gccagtaacc ttctgccata ctggcagccc gcagccacac ttcattatta
     8281 acgattttca cttcccgacc cggcagcggc gaaccaacgt ctgccaggcc gtcggcttct
     8341 ttcgcacaca ccgtggaggc aaactcggtc agaccatagc cgcaaaagca acgaatcccc
     8401 tgctcgcgcg cctgttccgt caactcgacc gggatagccg cgccgccaag taacaccgct
     8461 ttcagggaaa cggaactgcg gttaaccagc aaacgccaga gttgtgttgg caccagtgaa
     8521 gcatgagtac agcctgccag catttgctcc aatggctgtt tatcacgcac cgtcatccgc
     8581 gcaccagcgt ataaccagcg ccacataatt ccctgaccgg agacgtgaaa cagcggtaaa
     8641 gagagcaacc agtcatcgtg atcgccaaac ggaatcaacg ataacacccc ttcggcactg
     8701 gcaagatggg cctgataagt atggacagcg gctttcggca acccggtaga acccgaggtc
     8761 aacgtcattg agcacagacg cgtcggctgc cacgtagcgg catgtgcgcc ttcaacccgc
     8821 tgaatgtgca gcgacgttaa cgccggaaac gtgttttccc catccggcac cagcgcaaat
     8881 tgcagcgtca gattgggcag caattcttca agcaacggtt gcggcagctg agggttcacg
     8941 ggcaacaccc gcgccccgca ttgcagtaac gccagccagg cgagcagcgt ttgcggcgta
     9001 ttccacgccc gcaacatcac gccgctgccc tcaacaacgc cctgcgccgc aaagcctgag
     9061 gctaattcat cgacgcgagc acaaagctcg cgccagttaa gttgctcgtc attaagacgt
     9121 aaggcgatgg cttctccccg cacttgccgc cagtgacgcc acggccagtc agagaagatc
     9181 ataacaaccg ctccagtgca tcaacttcca cgacaggcag cgtgctaccc ggccagcgac
     9241 gtacctgctg cgcctgcatc agatccagcg tgtccagccc tggaatggtg tccggcgtta
     9301 accaggcggc aatccgcgcc agttgcgtta agcctaagct cgattcaatg gaagaactga
     9361 tcaccgccgt cagccccagc gcgtgcgccg cctgtacctg ctcgcgtact ttctccagac
     9421 tgcccgtgag cgtgggtttg ataactaccg cgcgcacgcc ctcttcagcc acaaaggcaa
     9481 aatccggctc gcgcaagctt tcatcccagg caatggcaat gccggtttca cgggcaaacg
     9541 ctcgcgaatc atcgcgggtt ttgcacggct cttcgagaaa cgcgatgcgg tggcgataat
     9601 ccgggttaac atatttggca aactgctggc ctttcagcgg tgtccaggcg cgatttgcgt
     9661 caagacgcaa atgcagatcc ggaattgcct ccagcaacag attaaccacc atgccgtcgc
     9721 gcacggcttc gtacaatccc actttgacct tcgccacttt ctcgcctggc atatcagcaa
     9781 gtttgaggat cagatcgtca ggatcgccat tacacagtgg tgccgcacgg taattggccg
     9841 cctgcgggag cgtatctgcc agctccgcca atgcacagct tacgccaaag gcaacggaag
     9901 gcatctgcgg tagctcgcaa tcgcctgcca gccagttatt tacccaggca agcagcacgc
     9961 tttgcgcctc ttccctggtt tcctggctga agcccggcag tggggagatc tccccccacc
    10021 cttcgcgctc gccctcacgc aggcaaacgt acagcccgtc gcgggttttt aaccgcctgt
    10081 cgcgcagaac cacccccgcg tccatgggga tctgccagcg gtatacctgc gcgctacgca
    10141 ttacggattc cgtttaaatt tgctgaaatc aggctgacgt ttctggttga aagcgttgcg
    10201 accttcctga ccttcttccg tcatgtagaa cagcatggtg gcgttgcccg ccagctcctg
    10261 cagccccgcc tgcccgtcac agtcggcgtt cagcgcagct ttcaggcagc gcagcgccat
    10321 cgggctgttt tgcagcattt cgcggcacca acggacggtt tctttttcca gatccgccag
    10381 cggtaccacg gtgttgacaa ggcccatatc cagcgcctgt tttgcgtcgt actgacggca
    10441 caggaaccag atttctcgcg cttttttctg cccgacgata cgagccatgt aggaagcgcc
    10501 ccagccgccg tcgaaggaac cgactttcgg gccagtctga ccgaagatgg cattatctgc
    10561 tgcgatagtc aggtcgcaca tcatgtgcag aacgtgacca ccgccgatgg agtagccagc
    10621 caccatcgcg acaaccggtt tcggacaggt acggatctga cgctggaagt ccagcacgtt
    10681 caagtgatgt acgccggaat catctttata gccgccgtaa tcaccacgca ctttctggtc
    10741 accaccggag cagaacgctt tatcgcctgc gccagtcaga atgatcacgc cgatattgtc
    10801 gtcataacgc gcatctgcca gtgcctggat catctctttt accgtcagag gacggaaggc
    10861 attgcgcacc tgcggacgat taatggtgat ttttgcgata ccgtcggtgg atttttcata
    10921 acgaatgtcc tcgaaacctt cggagcagtc gtgccattca accggtgcgt aaagcattgc
    10981 ttcatcagga taaatcatat tgtgtccttt agtcagaaac gcaagatctg cgccagactt
    11041 gcgattacgc cagcgggatt ttcccgatgc gcgttatgtc cggcgcgagg aatgacatgg
    11101 cagtcggcag ccagttccgc cgccagggcg cggaatttgc tgtcacgttc accacataaa
    11161 taataaaacg caaatgtgcg ggcgctaagg ttagcacgta aatcaggctg gacggcgaga
    11221 gaagtcgcct ccagcatggc ggcaagggtt gcgccattat tgttgctgcg cagcgccacc
    11281 agctcccggc gttgatcgtc attgagcgag gcaaaaacag gctgttgata ccagtcggca
    11341 aataccgccg ttaacggttc tgtgcaaaaa cgctgcgccc attggcgatc ggaacgctga
    11401 cgttccgcac gttgttcagc attttgcagc cccggatgcc cgccttcgac aataacccca
    11461 caaagccccg ccagcccctg gcaagccgcc atcatcgcca cccgtccgcc aagcgagtac
    11521 cccaccagcc agaagtcaag gatgttgtaa ctaaccaagg ttttacgcag taagtcggtg
    11581 acatcatcaa atccatcgac gctaatcgcc gccgaaccac catgacctgg gagatcaaca
    11641 tacaaccgtg agtagtcggc aaacgcctcg cccacttctt gccattcgtg gcaatcgccg
    11701 gaaaaaccgt gcaaaaacac cagccagggt aaacctggtt ttccgtgttt tgcctgcgcg
    11761 tgcaggatca taaatggctt acctgcgcca gaagttgctg gagcgtttgc gcaccatcgg
    11821 tgtcgttaac caccatttca atcaccgtgg tggttggcgt gcgccaggcg tcggcaaatg
    11881 ccgtttcaag ttcctgccag ttttgcggac gatgatattt cagctcgaac atcgcggcgg
    11941 cgtgctcaaa atggacgttt tgcggcatca gatagaaacg ctcacgctcg ctttgcggcg
    12001 ttggcaacag cgagaaaatt tgcccgccgt tgttgttcac cacaattaat accagcggcg
    12061 cagaaacctg acgcaataac gccagcgcgt tgagatcgta aagtgcggag agatcgccca
    12121 caatcgccag cgtcggtttg ccgcttgccc gctgaacgcc tgcggcggtc gaaagcagtc
    12181 cgtcgatacc gctggcccct cggttgctgt acaccgggta acctgccgga agttgcgaaa
    12241 gcgcatcaat cagacgtacc accaggctgt taccaacaaa caattgcccc tgttcaggca
    12301 gatagtcgct gatgcgatgc gccagttgcg cttcgccaaa cgcatcacgg cgggcaataa
    12361 ccgcctgcat tgcctgttcc gccaggcgcg ggatttcaac gcaccagggc tggcgttttt
    12421 ctgccggatg cagctccagc cagtcggcaa tattggcaat taagcgacgt ccgcgatggt
    12481 gtgccggatc aagtcgccct tcaatgtcat caacaatcca gtactcttcg ggttcacagc
    12541 ttgcctgcca ttgcaggagc cgtttccccg tcaggctgct tcccagttgc accacaattt
    12601 gcgcctgctg cagctcgctg gtcgctttgg cattgcctaa ccaaagatcg gcacacggca
    12661 gcggctgccc ggtttgtgac agcacatcgc caatcagcgg ccagccaaga gtttgcgccc
    12721 acagggcaac ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc acgccgcgct
    12781 tttgtcgcca gaagaaccag tcgcgctgtt tttcactttc cagacgaggc gcttcacgca
    12841 gccacggttt gtcgtcctgc caccagtcac ccagacgctg ttgccagcta agcccggtat
    12901 cgtccatttc gccatacagc ggttcagcaa acgggcagtt gatatggact ccccccgcat
    12961 gaagcgtacc gagagcgtgg tcgatggtag aaaccagcca acgtgcgggg atatcctggg
    13021 tcgggcgcgg caatgaaata ctgtgcgtgg ggtgagaggc gaacattccc ggctggcgaa
    13081 ttgcctgatt cgcgccgcag tcaattagct ccggcgggcg atcggcggtt aagagaatca
    13141 gtttttctcc ggttaacccg gcttcaatca gtgccggata gagatttgcc accgccgtgc
    13201 cggaggtcac aatcaccgcc accggctgct tgctgacttt cgccagcccc agcgccagat
    13261 gccccagtcc acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc tccgccgccg
    13321 ctaacgttaa cggtgtagaa cgcgagcctg gggcgataca gatgtgtctg acgccgtgac
    13381 gcgttaatgc ttccagaatg accgccgccc agcgtcggtt aaatgcgctt actgacatga
    13441 gtttgtccgg tatcaatatt gcggctaagt ataaggagct acaaaaatag attattgata
    13501 tgaatcggta atgatgcgac tcattactat tccatttgta ataaagtacg cagccctgcc
    13561 gctttgttgt cgatttcctg ccactcttgc tcggggtcgg aaccacggac aatgcccgcg
    13621 ccagcatata gccgcacgac attgccgctg atttttgctg aacgcaggga aacgcagaat
    13681 tcgctttgtt gtaatgagag atagcccgca gaaccggcgt accattctcg ggtgaacggt
    13741 tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta agccagcaac tgctgccgtt
    13801 ggctgcaact gatgtaaaca gatcacatca tccgctttgt tgagtgaagt ccagatacag
    13861 cggcgaagat gctgcacttt acgcagacgc agtacctgcg gcggtaaaac atccagcgtc
    13921 tgggtatcgg cctgtaatcg ttggcagata tcttccacca ccagcatgtt ctcgcgctgg
    13981 tttttatcat ccgccatcag ccactctcct aactgctgcg cctgcttatc atcaggatta
    14041 tttgctactg ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg ccgccataac
    14101 cgttccggtg aagagccaag aaaagcattt tcgccatcaa aggccatgta aaaatggtag
    14161 caattcagat tcagtcgacg actggcagcc atcatcgccg ccgcgttgac cggacttgcg
    14221 aaatgcaggt cagttgcccg agcgagcacc actttgtcga gctcaccttc ggcgatggtt
    14281 ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt ccggccagtg ttgttctcgc
    14341 gtggtggtta aatgtaaccc aggcaagggc ttgatactca ccagtgtggc gataaattct
    14401 tttgcctgaa tcgcatcgtg ctgaagggag ctttcgctga atagcgtcag ccgcagcgtg
    14461 gctttaccgc cacagcgtcg ccattccagg cggggtaaaa gtaaattgcc ctgcgacggg
    14521 tcaaaagcat tcagccccca aatgcgtaag tcggcgtgtt ccgggtgctg gcgaagaaaa
    14581 cgttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacggc tgcttcttca
    14641 tcaccattac gttgttgcca gtagaattgc gggtacgttt gctgactggc cagccagctc
    14701 aaggcatcaa aagcgtcttt gagagggaaa ggaatatcga taacccgaat gccgggtgtc
    14761 gccggaatct cttgcgacaa atggcgcagt agattttcca gcgccgtagt aagtgattgc
    14821 acgcggacct ctccctgtta aaagcctcac attatacggg gtactacaaa aaaaatgcag
    14881 taccccggtg tagggaggtt ttaacggcgt gccagcaaca gtcctagtac cagcccaacg
    14941 gccgcgccca caccaattcc ttgccagggt ttttcgtgga cgtagtcatc agcacgataa
    15001 actgcctgct tcgcccgata gtaataactg tctgaagcct ggctaacccg ttttttcaca
    15061 tcatccagcg ctttttctgc acgcgctttc agctcaacat atttctgatc ggcgggatcg
    15121 ccagaggagc ggagcacctc ttccagtgtt tcactaagca gcgtcaggtc gtcatcgata
    15181 cgtgtatcac caaactgatt agacattctc gttctccatt tgcgtaaaac ctgtcactaa
    15241 ctatagacaa gggttgttga ttacgcctga attacctcgc gtgccatccc aatgtgtggg
    15301 ataccatctt cttcatagac ctccgtcaca gggataaaac cgaaactctg gtagaagttt
    15361 tgcagatgcg cctgcgcccc gaggtaaaca ggcttatcag gccagtgatg cgtacatgtt
    15421 tcgagtgttt tactcatcag ctgctgcccc actttttcgc cacgcaacgc ttcactgaca
    15481 atcacccgac ctataacgac cggctcaaga tcatcatcac ttttcagaat cctcgcatat
    15541 gccaccagtt catcattttt ccaaccgaga atatggcgat tatcccccgt caaatcatca
    15601 ccatcgatat cctgataagg gcaattctgc tcgacaacaa acaccgcgca acgcagttgt
    15661 aataaggcat aaagctggga aaccgataat tcagagtgat gcagatcttg ccattcaatc
    15721 ataatgctct ccttttagat gtatgcctta tactagccgg aatatttttt gaaacgtgat
    15781 gaactcatgg aattaatttt tttaggtact tcggccggtg tgccaacccg cacgcgcaat
    15841 gtcacggcaa tattgcttaa cctgcaacat ccgacccaga gcggtctttg gttgtttgac
    15901 tgcggtgaag gcacccagca tcagctactg cataccgcct ttaaccctgg aaaactggac
    15961 aagattttta tcagtcacct tcatggcgat catctttttg gtttacccgg cttgctgtgc
    16021 agtcgttcta tgtcaggcat tatccaaccc ttaacgattt atggtccgca aggtatccgt
    16081 gaatttgtgg aaaccgcgct gcggattagc ggctcatgga ccgattatcc gctggaaatt
    16141 gtcgaaattg gcgctggcga aattctcgat gatggcctgc gcaaagtaac cgcttatccg
    16201 ctggaacacc cactggaatg ttatggctat cgtattgaag aacatgataa accgggtgca
    16261 ttaaatgccc aggcattaaa agctgctggc gtgccgcctg gcccactgtt tcaggaatta
    16321 aaagcgggca aaacaatcac gctggaagat ggaaggcaga ttaacggcgc agattactta
    16381 gctgctccag tgccaggtaa agcgctcgct attttcggcg ataccggccc ctgcgatgcc
    16441 gcacttgacc tggctaaagg tgtcgatgtc atggtgcacg aagcgacgct ggatataacc
    16501 atggaagcca aagccaatag tcgcggccat agctctacac gccaggctgc gacactagcc
    16561 cgtgaggctg gagtcggtaa gctaatcatt acccacgtca gctcgcgcta tgatgacaaa
    16621 ggttgtcagc acctgttacg tgaatgcagg tcaattttcc cggcgactga actagcgaat
    16681 gatttcaccg tgtttaacgt ttaactctgc ttattattaa aaacagggcg aaacttgccc
    16741 tgttatcgca acccgcgcta catatatcca cccacacggg catattgagc gatttttgat
    16801 atttattcat ctccttacgc actatattca gagcaataag ttgcctcttt ttatcttcat
    16861 tcagcacaac ctttgcaact aaaaggagcc catgatggtt acagttgtca gcaattattg
    16921 tcaattatct caaacccaac tcagtcagac atttgcagaa aaatttactg tgaccgagga
    16981 attactgcag tctttaaaaa aaacagcgtt atccggagat gaagaaagta ttgagttact
    17041 gcataatatt gcgttaggtt atgatgaatt tggaaaaaaa gctgaagata ttctttacca
    17101 tattgttaga aacccaacaa atg
//
LOCUS       DAZFRJ010000092        17026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000092 DAZFRJ010000000
VERSION     DAZFRJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.092"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..234
                     /locus_tag="ACHKUN_002768"
     CDS             <1..234
                     /locus_tag="ACHKUN_002768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6660999.1"
                     /translation="SFIMISVFTTSPFRQDRPKFHAYTICVLAIDPFLTLRVVFPAYR
                     NTFVVRKVCKGKRLPCDFAGAEVRVWSEMEWQQ"
     gene            222..1247
                     /gene="purR"
                     /locus_tag="ACHKUN_002769"
     CDS             222..1247
                     /gene="purR"
                     /locus_tag="ACHKUN_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO6661000.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(1244..2176)
                     /gene="punR"
                     /locus_tag="ACHKUN_002770"
     CDS             complement(1244..2176)
                     /gene="punR"
                     /locus_tag="ACHKUN_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO6661001.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            2289..3500
                     /gene="punC"
                     /locus_tag="ACHKUN_002771"
     CDS             2289..3500
                     /gene="punC"
                     /locus_tag="ACHKUN_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO6661002.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            3791..4939
                     /gene="cfa"
                     /locus_tag="ACHKUN_002772"
     CDS             3791..4939
                     /gene="cfa"
                     /locus_tag="ACHKUN_002772"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO6661003.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(4979..5620)
                     /gene="ribE"
                     /locus_tag="ACHKUN_002773"
     CDS             complement(4979..5620)
                     /gene="ribE"
                     /locus_tag="ACHKUN_002773"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO6661004.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            5835..7208
                     /gene="mdtK"
                     /locus_tag="ACHKUN_002774"
     CDS             5835..7208
                     /gene="mdtK"
                     /locus_tag="ACHKUN_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO6661005.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            complement(7249..8505)
                     /locus_tag="ACHKUN_002775"
     CDS             complement(7249..8505)
                     /locus_tag="ACHKUN_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6661006.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEERA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            8813..8889
                     /locus_tag="ACHKUN_002776"
     tRNA            8813..8889
                     /locus_tag="ACHKUN_002776"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8847..8849,aa:Val,seq:gac)
     gene            8894..8970
                     /locus_tag="ACHKUN_002777"
     tRNA            8894..8970
                     /locus_tag="ACHKUN_002777"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8928..8930,aa:Val,seq:gac)
     gene            9078..9383
                     /locus_tag="ACHKUN_002778"
     CDS             9078..9383
                     /locus_tag="ACHKUN_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO6661007.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            9509..11113
                     /locus_tag="ACHKUN_002779"
     CDS             9509..11113
                     /locus_tag="ACHKUN_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO6661008.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(11125..11937)
                     /gene="ydhT"
                     /locus_tag="ACHKUN_002780"
     CDS             complement(11125..11937)
                     /gene="ydhT"
                     /locus_tag="ACHKUN_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO6661009.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(11941..12726)
                     /gene="phsC"
                     /locus_tag="ACHKUN_002781"
     CDS             complement(11941..12726)
                     /gene="phsC"
                     /locus_tag="ACHKUN_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO6661010.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(12723..13391)
                     /locus_tag="ACHKUN_002782"
     CDS             complement(12723..13391)
                     /locus_tag="ACHKUN_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6661011.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(13455..14093)
                     /locus_tag="ACHKUN_002783"
     CDS             complement(13455..14093)
                     /locus_tag="ACHKUN_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO6661012.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(14106..16208)
                     /locus_tag="ACHKUN_002784"
     CDS             complement(14106..16208)
                     /locus_tag="ACHKUN_002784"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO6661013.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(16229..16855)
                     /locus_tag="ACHKUN_002785"
     CDS             complement(16229..16855)
                     /locus_tag="ACHKUN_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO6661014.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         4110 a         4434 c         4022 g         4460 t
ORIGIN      
        1 tcatttataa tgataagtgt ttttaccact tccccttttc gtcaagatcg gccaaaattc
       61 cacgcttaca ctatttgcgt actggccatt gaccccttcc tgacgctccg tgtcgttttt
      121 ccggcgtacc gcaacacttt tgttgtgcgt aaggtgtgta aaggcaaacg tttaccttgc
      181 gattttgcag gagctgaagt tagggtctgg agtgaaatgg aatggcaaca ataaaagatg
      241 tagcgaaacg agcaaacgtt tccactacaa ctgtgtcaca cgtaatcaac aaaacacgtt
      301 tcgtcgctga agaaacgcgc aacgccgtgt gggcagcgat taaagaatta cactactccc
      361 ctagcgcggt ggcgcgtagc ctgaaggtta accacaccaa gtctatcggt ttgctggcga
      421 ccagcagcga agcggcctat tttgccgaga tcattgaagc ggttgaaaaa aattgcttcc
      481 agaaaggtta caccctgatt ctgggtaatg cgtggaacaa tcttgagaaa cagcgggctt
      541 atctgtcgat gatggcgcaa aaacgcgtcg atggtctgct ggtgatgtgt tctgagtacc
      601 cagagccgtt gctggcgatg ctggaagagt atcgccatat cccaatggtg gtgatggact
      661 ggggtgaagc aaaagctgac ttcaccgatg cggtcattga taacgcgttc gaaggcggct
      721 acatggccgg gcgttatctg attgaacgcg gtcaccgcga aatcggcgtc atccccggcc
      781 cgctggaacg taacaccggc gcaggccgcc ttgccggttt tatgaaggcg atggaagaag
      841 cgatgatcaa ggtgccggaa agctggattg ttcagggtga ctttgaaccc gaatctggtt
      901 atcgcgccat gcagcaaata ctgtcgcagc cgcatcgccc tactgccgtc ttctgtggtg
      961 gcgatatcat ggcaatgggc gcactttgtg ctgctgatga aatgggtctg cgcgtcccgc
     1021 aggatgtttc gctgatcggt tatgataacg tgcgcaacgc ccgctatttt acgccggcgc
     1081 tgaccacgat ccaccagcca aaagattcgc tgggtgaaac agcgttcaac atgctgttgg
     1141 atcgtatcgt caacaaacgt gaagaaccgc agtccattga agtgcatccg cgcttgattg
     1201 aacgccgctc cgtggctgac ggcccgttcc gcgactatcg tcgttaatca cccgttgcgg
     1261 gagtctcttc cggctcccgc agccactcct tattcagcgt ctcactatcg ccgagatact
     1321 caagcagcca ggttaacgca ggcgacatat cattttgctg ccatgtcaga caacatgccg
     1381 aatccggaaa ggggttttcc agttctaatg ccacccactt cccctcatta agccacggtt
     1441 tggcgaaatg tgttggcacc atccctatgc ataatcctgc cgagatacag gttgccgatg
     1501 attcccagtc aggcacgaca actctttttt ggttatccag caaccaggta atacgtttag
     1561 gtagcgttcg cgaggtgtct tcgcgcacca acgacggcca gttgcgcaac gtatcatcgc
     1621 tgaacgggcc atccatcaac gccagcgggt ggtggctggc aacaacgcaa ctccagctta
     1681 gcatccccat atcccggaag gcataacgac cgcctaccgg aatcgcgcgt gttgcgccaa
     1741 tcgccagttc cacgcgccca tcggaaagcg catcccagac accgttaaac acttcctgaa
     1801 agaccagaag ttcgacgtcg tcaaaatggc gataaaaatc aacgatcatc tgccgtgtac
     1861 gttctggcct gacaatatta tccactgcga tagctaactg accgcgccag ccgttcgcta
     1921 tctgctgaca ttgctggcgg gtgatctgca tttttttgac aacagagcgc ccttctttga
     1981 gaaaccacgc tccagcagcg gtcagctcca catcacggtg ccgtcgttca aagagcggta
     2041 ccgccagcca ctcttccagc tgacgcacgg tatagctgac cgcagaagga acgcgatgca
     2101 gctcctgtgc cgcagcgcta aaactaccat tacgcgctac cgcatcaaca acttcgagtg
     2161 aatattctga ccacatagtc tgcctgcaaa atttttgaaa ccagtcatca aatattaccg
     2221 tttcacaaca ctaatttcac tccctacact ttgcggcggt gtttaattga gagatttaga
     2281 gaatatacat gcaacctggg aaaagatttt tagtctggct ggcgggtttg agcgtactcg
     2341 gttttctggc aaccgatatg tatctgcctg ctttcgccgc catacaggcc gacctgcaaa
     2401 cgcctgcgtc tgctgtcagt gccagcctta gtctgttcct tgccggtttt gccgcagccc
     2461 agcttctgtg ggggccactc tccgaccgtt atggtcgtaa accggtgtta ttaatcggcc
     2521 tgacaatttt tgcgttaggt agtctgggga tgctgtgggt agaaaacgcc gctacgctgc
     2581 tggtattgcg ttttgtacag gctgtggggg tctgcgccgc ggcggttatc tggcaagcat
     2641 tagtgacaga ttattatcct tcacagaaag tcaaccgtat ttttgcgacc atcatgccgc
     2701 tggtgggtct gtctccggca ctggctcccc tgttaggaag ctggctgctg gtccattttt
     2761 cctggcaggc gattttcgcc accctgtttg ccattaccgt ggtgctgatt ctgcctattt
     2821 tctggctcaa acccacgacg aaggcccgta acaatagtca ggatggtctg acctttaccg
     2881 acctgctacg ttctaaaacc tatcgcggca acgtgctgat atatgcagcc tgttcagcca
     2941 gtttttttgc atggctgacc ggttcaccgt tcatccttag tgaaatgggc tacagcccgg
     3001 cagttattgg tttaagttat gtcccgcaaa ctatcgcgtt tctgattggt ggttatggct
     3061 gtcgcgccgc actgcagaaa tggcaaggca agcagttatt accgtggttg ctggtgctgt
     3121 ttgctgtcag cgtcattgcg acctgggctg cgggcttcat tagccatgtg tcgctggtcg
     3181 aaatcctgat cccattctgt gtgatggcga ttgccaatgg cgcgatctac cctattgttg
     3241 tcgcccaggc gctgcgtccc ttcccacatg caaccggtcg cgccgcagcg ttgcagaaca
     3301 ctctgcaact gggtctgtgc ttcctcgcaa gtctggtagt ttcctggctg atcagtatca
     3361 gcacgccatt gctcaccacc accagcgtga tgttatcaac agtagtgctg gtcgcgctgg
     3421 gttacatgat gcaacgttgt gaagaagttg gctgccagaa tcatggcaat gccgaagtcg
     3481 ctcatagcga atcacactga tctatatcga tatacttata cttaggctgc taacaaaatt
     3541 ttgttgtatg attgaaatta gcggcctata ctaatttcga gttgttaaag ctacgataaa
     3601 tattatgttt ttacggggac aggatcgttc ccgactcact atggatggtc atttcggcaa
     3661 gggttcctcc tttccctctg ttctacgtcg gattatagac tcgcggtttt ttctgcgaga
     3721 tttctcacaa agcccaaaaa gcgtctacgc tgttttaagg ttctgatcac cgaccagtga
     3781 tggagaaact atgagttcat cgtgtataga agaagtcagt gtaccggatg acaactggta
     3841 ccgtatcgcc aacgaattac ttagccgtgc cggtatagcc attaacggtt ctgccccggc
     3901 ggatattcgt gtgaaaaacc ccgatttttt taaacgcgtt ctgcaagaag gctctttggg
     3961 gttaggcgaa agttatatgg atggctggtg ggaatgtgac cgactggata tgttttttag
     4021 caaagtctta cgcgcaggtc tcgagaacca actcccccat catttcaaag acacgctgcg
     4081 tattgcaggc gcccgtctct tcaatctgca gagtaaaaaa cgtgcctgga tagtcggcaa
     4141 agagcattac gatttgggta atgacttgtt cagtcgcatg cttgatccct tcatgcaata
     4201 ttcctgcgct tactggaaag atgccgataa tctggaatct gcccagcagg cgaagctcaa
     4261 aatgatttgt gaaaaattgc agttaaaacc agggatgcgc gtactggata ttggctgcgg
     4321 ctggggcgga ctggcgcact acatggcatc taactatgac gtaagcgtgg tcggcgtcac
     4381 catttctgcc gaacagcaaa aaatggctca ggaacgctgt gaaggcctgg atgtcaccat
     4441 tttgctgcaa gattatcgtg acctgaacga ccagtttgat cgtattgttt ctgtggggat
     4501 gttcgagcac gtcggaccga aaaattacga tacctatttt gcggtggtgg atcgtaattt
     4561 gaaaccggaa ggtatattcc tgctccatac tatcggttcg aaaaaaaccg atctgaatgt
     4621 tgatccctgg attaataaat atatttttcc gaacggttgc ctgccctctg tacgccagat
     4681 tgctcagtcc agcgaacccc actttgtgat ggaagactgg cataacttcg gtgctgatta
     4741 cgatactacg ttgatggcgt ggtatgaacg attcctcgcc gcatggccag aaattgcaga
     4801 taactacagt gaacgcttta aacgaatgtt tacctattat ctgaatgctt gtgcaggtgc
     4861 tttccgcgcc cgtgatattc agctctggca ggtggtgttc tcacgcggtg ttgaaaacgg
     4921 ccttcgagtc gctcgctaaa ggctattcta tcgccccctc ttcgggggcg agttcagatc
     4981 aggcttctgt acctggttga ttcatggcat tttctcgtgc cgccagcaca cgttctaccg
     5041 tatctaccac tgcctgagtt tgtggatcaa tttcaatgtt gacgcgtgcg ccgagttttt
     5101 tcttcccaag agtcgtgcgt tccagtgttt ccggaattaa atggacgcaa aaacgcgttg
     5161 gcgtgacttc gccgacggtc aggctaatac cgtcgatgcc aataaatcct ttgtacagaa
     5221 tatatttcat caactgacta tcctggactt taaaccagat ctggcgatta ttttctgagg
     5281 ttaatatttt cgccacttca gcagtggtca taatatgacc tgacattaag tgtccgccaa
     5341 tttcatcact gaatttcgcc gcacgctcaa cgtttaccca atcccccact tttaaatcgc
     5401 caagattggt aatgcgtaac gtttctttca tcaggtcaaa actgacatgg ttgccgttaa
     5461 tttccgtcac ggtcaggcag caaccgttat gcgccacgga cgcaccggtt tccaggccgt
     5521 ccagcatgtg gtcgggtaac tccaccacat gcgtacgaaa atttggtttc tcgtcaatcg
     5581 acaccagttt tgcggtgccc tgtacaatcc ccgtaaacat acttacaact cctgaaatca
     5641 gttaagacat tctgttcagc acaatagcag gtggcaaacg cccttaccag tgaaggggta
     5701 agaatgacta ttttttcact ggagaattaa taaatcctcg ctacaataga ctgaatttcc
     5761 cctgcttctt ctttttgctg cccattcagg cggcttttta gtctctcata taactacaaa
     5821 taaaaggtgt tcacgtgcag aagtatatca gtgaagcgcg tctgttatta gcattagcaa
     5881 tcccggtgat tctcgcgcaa atcgcccaaa ctgcgatggg ttttgtcgat accgtgatgg
     5941 cgggcggcta tagtgccacc gacatggcgg cggtcgctat cggtacttct atctggcttc
     6001 cggcgatcct ctttggtcac ggactgctgc tggcattaac gccggttatc gcgcaattaa
     6061 atggttccgg tcgacgtgag cgcattgcgc atcaggtgcg acaaggtttc tggctggcag
     6121 gttttgtttc cgttctcatt atgctggtgc tgtggaatgc aggttacatt atccgctcca
     6181 tggaaaacat cgatccggct ctggcggaca aagccgtggg ttatctgcgt gcgttgttgt
     6241 ggggcgcgcc gggctatctg ttcttccagg ttgcccgtaa ccagtgtgaa ggtctggcaa
     6301 aaaccaagcc gggtatggta atgggcttta tcggcctgct ggtgaacatt ccggtgaact
     6361 atatctttat ttatggtcat ttcggtatgc ctgagctcgg tggcgttggt tgtggcgtgg
     6421 ctactgcggc ggtgtactgg gttatgttcc ttgccatggt ttcttacatt aaacgcgccc
     6481 gctccatgcg cgatattcgt aacgaaaaag gcaccgcgaa acccgatcct gcggttatga
     6541 aacgactgat tcaactcggt ttgccgattg cgctggcact gttctttgaa gtgacactgt
     6601 ttgctgtcgt ggctctgtta gtgtctccgc tcggtattgt tgatgtcgca ggacaccaga
     6661 ttgccctgaa ctttagttca ctaatgttcg tgcttcccat gtcgctggcg gcagcggtaa
     6721 ctatccgcgt aggttatcgt ctgggtcagg gctcaacgct ggatgcgcaa accgctgcgc
     6781 ggaccgggct tatggtgggt gtctgtatgg caaccctgac ggccatattc acggtttcac
     6841 tgcgggagca aatcgccctg ttgtacaacg acaatcccga ggttgtaacg ctggctgcgc
     6901 atttgatgtt gctggcggcg gtatatcaga tttctgactc gatccaggtg attggcagtg
     6961 ggattttgcg tggttataaa gatacgcgtt ccattttcta tattaccttt acggcttact
     7021 gggtgctggg cttgccaagc ggctatattc tggcactgac cgatctggtc gttgaaccta
     7081 tggggccagc aggcttctgg ataggcttta ttattggcct gacgtcggca gccattatga
     7141 tgatgctgcg tatgcggttc ctgcaacgtc tgccgtcagc catcattctg caacgagcgt
     7201 cccgctaata aagacaaggc gcaaccttca cgggttgcgc ctgtgttttt acgcaggctg
     7261 gagcgttgcg ccaatcccgt cttcgtctgg ctgtagtttc agtgcgttac acagagtttg
     7321 ccgactggca tctgttatct caacgcgtat atccccggta acttcccctt tcacattgat
     7381 gaagtcaggt gagatttctt tcgcactggt ttgcaggcaa atcgctccct ctccgcgcag
     7441 gcgtttcact ttcagttgca catgcgttgc cgcaccagtg atcagccaca gttttccgcc
     7501 attcagattg atgttaccaa cactaaaagt gacactgtca ccttttaccg tactgccgtc
     7561 gagaatcaac acaccgccct gctgtacagt aatcgttgat tgttgcagat ctgcaccata
     7621 gccaataatc attgaagcgc cgtccagtaa tgcaacactc ccggtcagtt caaccggtgc
     7681 gcgctcttcc tctacgacaa aattttcgtc cgcgctggtg ggtgacaaca ggatcactgt
     7741 tccaccttgt attaccgcat tttccagcgt accggcatgc acttcagccc gaccacccac
     7801 attcacggac aggttttgcg tcttaccgga actgtagagc tgaaggccat ccgtccccag
     7861 acgataaatg gatccattct caacaatagt gtcagagtct tcaccaccga gattattcat
     7921 ggtgccacca gcaagcaacg tatcactggc tttcccggcc agcataaaaa cgccgccctg
     7981 attaatttca caattgctgg cttccccacc tggatacacc aacaattcac caccttcatc
     8041 taccacgaca gctgaggttg tcccattgac caacaggccg ccctcttgat cgagaatgat
     8101 tttttccgcg cgatgtcctt ccagtacacg caggttaccg ccattttcca gcaacaaacc
     8161 gcaggcataa ccctgatcaa cgctgaattc accttcggga tggcggccat taaccgtagc
     8221 gagcgtagag agactaattg cgcctccctc ggactgaaca atatctgtgg caatggcacc
     8281 gtcgctgacc gatagcgtgc cattgttacc gtactgtgtg catttggcga tcccaccggt
     8341 tgccactttc aaccagccag cccccgtgac cacggtattt tcggctcttc cgccagcttc
     8401 aacaatcaac tcgccttcag taagttgcgt cgcgccaatg acctcgccgg tcttaacaat
     8461 ttgcacattc ccgtcgctca tcaagttttt cgcatcagat cccatgattt attcctttgc
     8521 tgcatctgtg tgcctttatt gctacctaag tgtaaaggct acggaggatt tatccacgac
     8581 agatttgaga tggtggcaaa caactctgtt taaactctga tacacgaatt attgggttgt
     8641 atcagatgta aatgcgatcc tgaataaaaa tcgcccttgc aaatcaacaa aatatagcca
     8701 atttgcttaa aagctctgca aacggtcgaa atagcgcaga aaattacgtt ttgcctcttg
     8761 ccaccttccc actctgccgc taatattcgt ccccgttgtc acctacaacg ttgcgttcat
     8821 agctcagttg gttagagcac caccttgaca tggtgggggt cgttggttcg agtccaattg
     8881 aacgcaccat cctgcgtccg tagctcagtt ggttagagca ccaccttgac atggtggggg
     8941 tcggtggttc gagtccactc ggacgcacca gattttctta atctggtctt ctcctttttt
     9001 cctctgtttc ttctctgtat ccaatacgtt aaaagattta cactgtcttc gtatgcgtta
     9061 tcagaaggag aattgctatg gcaactttgt tacaacttca ttttgctttt aatggcccat
     9121 ttggcgacgc aatggctgag cagcttaaac cacttgctga gtcgattaat caggaacctg
     9181 gttttctgtg gaaggtatgg acagaaagtg aaaagaacca cgaagccggt gggatctatc
     9241 tttttactga tgaaaaaagc gcccttgcct atctggaaaa acatactgcc cgacttaaaa
     9301 atctcggcgt tgaggaagtt gtcgccaaag ttttcgatgt caatgagcca ctgagtcaaa
     9361 tcaatcaggc aaaactcgcc tgacagaatt taatcaaggg cggttagcgc ccttttcata
     9421 cctgtctgaa atttctcaaa ttctaaaaat ctcaaccaaa cttatctgat aacactaaat
     9481 tcgaaagaat gcgtacaggt aagtaacaat gaaaaaaatt gctattgtgg gtgccgggcc
     9541 tacggggatc tacaccttat tctcgcttct acagcaacaa actccacttt ctatttctat
     9601 cttcgagcag gctgacgagg ccggtgtcgg gatgccatac agtgatgagg aaaactcaaa
     9661 aatgatgctg gcaaatattg ccagtattga aataccgccg attaattgta cgtatctcga
     9721 atggctacaa aagcaagaag ccagccatct ccagcgttat ggcgttaaaa aagaaacctt
     9781 gcacgatcgt cagtttttac cgcgaattct gctgggcgaa tatttccgcg atcaattttt
     9841 acgattagta gaccaggcac gacagcaaaa atttgcagtg gctgtttatg aatcatgcca
     9901 ggttaccgat ctgcaaatta caaatgctgg cgtcatgctc gctacaaatc aggatttacc
     9961 cagcgagacg tttgatttag cggtgattgc cacgggtcac gtctggcctg atgaagaaga
    10021 agcaaccaga acgtattttc ccagtccgtg gtcaggcctg atggaagcaa aggtcgatgc
    10081 gtgtaacgtg ggtattatgg gaacatcctt gagcggactg gatgcggcaa tggcagtggc
    10141 tattcagcat ggttcgttca ttgaagatga taaacaacac gtcgttttta accgcgataa
    10201 cgcaagtgaa aagctaaata ttacgttgat gtcgcgcacg ggtattttac ccgaagccga
    10261 tttctattgc cctattccct acgagccctt acacatcgtc accgatcagg cattaaatgc
    10321 tgagattcaa aaaggtgaag agggcctttt ggatcgggta tttagattga tagtagagga
    10381 aatcaagttt gctgatccag actggagcca acgcatagcc ttagagagcc tgaatgtcga
    10441 ttcctttgct caagcctggt ttgccgagcg caaacaacgc gacccatttg actgggcaga
    10501 aaaaaatctc caggaagtcg aacgcaataa acgagaaaaa catactgttc cctggcgtta
    10561 tgtcattctg cgcctgcatg aagccgtaca ggaaattgtt cctcatctga atgaacacga
    10621 ccataaacgg ttcagtaaag gccttgccag ggttttcatc gataattatg cggcaatccc
    10681 ttcagagtct attcgtcgcc tacttgcctt acgtgaagcg ggaatcattc atattctcgc
    10741 cctcggtgaa gactacaaaa tggaaattaa tgagtcgcgc accgtcctga aaacggaaga
    10801 caacagctac tcgtttgacg tttttattga tgcccgcgga cagcgtccgc ttaaagtgaa
    10861 agatattcct ttccctgggc tacgcgagca attacagaaa acaggggatg aaatccctga
    10921 tattggcgaa gattatacgt tacagcaacc cgaagatatt cgtggacgcg tagcgttcgg
    10981 cgcgttgccc tggttgatgc acgaccagcc tttcgttcag ggacttacgg catgtgcaga
    11041 aattggtgag gcgatggctc gggcggtcgt gaagcctgca tcccgtgctc gtcggcgtct
    11101 ttcgtttgat taaaggtaaa gctatcagca ctcaacaaac tgatgctgtt cattaagacg
    11161 atagcggact ccggttcgaa tattgttttc accttcaatg gcgacggcga agcgaactct
    11221 ttcgccgtca tgatatgcca gtgcggcgct tccccctggc ccgagaataa ttgagtcgac
    11281 cacgcccgtg ctcactaccg ttgaattttc accgctggca atgatgtgta cattgtcgcc
    11341 gctgttggca attctggcgt ttgcgccaaa gctggcgatt tgcaccaggt caccattact
    11401 ggcgacatga caccgttcac ccaatgtaca aacgcgtact cgcattccgg tattcgcaat
    11461 ccgactacta tctccagcgc tgcttatccg cgaagaatta ccggttacag caacccgtgc
    11521 gcgttcaccg gagctgccaa tgtgactgtt aaagccgaca cttccaatcc gcacgctata
    11581 acccatactg gcaatttgcg cggcataccc agcggttgcg attcgggcgt tatcctcaca
    11641 acagccgatg cgagcatgat cgtcgctgcg ggcaaactga ctgcaaaatg gtatctctcc
    11701 ggggtctgtg gccagttttt gcatagagga aacttcctga tgcgcgaagt tttcatccgc
    11761 cagccatttt ttccacgcat actcaaccag actctccgcc cagtccgtat atccttcctc
    11821 aatcagtgca tgatgaatat cggcataact accgccgcgt gggaaatggc gcaggaacca
    11881 gcgatacatc accgctccga tacgccattc tcgcaggtct gctcgtgtaa tgatcattcg
    11941 ttagtgccgg tgatagccat cgaccatgct tttaaaggtt tcgtgtggcg tacgccccgt
    12001 ggtgcaaaga taaagatgac cgaagataaa aaagaggctt ataaatgcca gagcaaaatg
    12061 cgcctgcaat aaccagtatc ttacgccagg aaacacatct cccacggctt gcggatagag
    12121 acacagcagc cccgtcaata gtaacaacgg cagcaatcca tacataacac caacataggc
    12181 gacctgctgt aagggattaa atttagactg ggttgttgcc gggaaaggat gttcttcccc
    12241 ctgcataatg ccaaacaaat aaaatcgcgt ttgttttgcc gctcgctcca gccacccctg
    12301 acgacgaatg cgatagtggt gaccattacc cccaacggca ttgatcagca caaagccgag
    12361 ccagcatgcg agtaacaaaa atccgcaaac ttcatgcacc gcaaccagac ttttaaccgc
    12421 agttgcgccc accagcgcaa agtgatttat cagcccactt gccagcaaca atacaaagag
    12481 taacgcattc gaccagtgcc ataatctgac cgctttgcag taaagataaa tcttctcacc
    12541 ataatcggtg actgatttct tcacgccacg agcacgaagc aaggcatgca gccccagcac
    12601 caaccacatc ccaaccagca atactccggc aatgatcaac cacaccggcc aaaattcggg
    12661 agtgaactgt ggcacatagt tcgctaactg gctctgaaac tgttcagcat gttgcgacgg
    12721 gttcatacat tttccttttt aatcaaatgt tgaccgaacc ggcgatacag atgcggtttc
    12781 ccggcaccag gtagctgata ttgatagtat ttattttgct gcaaccacgc ctgaatttct
    12841 gggctatctt cgcgaccaaa gatcaatgcg tgttccgggc aggcgctgac gcaaatgggc
    12901 ggaaagcctt ttgccagtcg ggactcagcg cagaaatcac atttatctgc cactttagtg
    12961 acagggttaa ggtagcgaac ctggtacgga caagcgccaa tgcaataact gcagccgatg
    13021 cactgcgatt tttctacccg cacgatcccc tgctcatcac gccacgacgc ccctgtcggg
    13081 catacgtcga tgcacggagc atcttcgcag tgctgacatg actggcgaaa aaagtgatac
    13141 tgcgtttcgt tgtcgttatc ggtgactgga atgtgtgcta tcgataaacg gcttccctga
    13201 gcggggacgt ggttagtttt acggcatgcg cgggcgcaaa tattacagcc attgcaccgt
    13261 gactcgtcat gaatcatggc ataccgaact tccttttctt gcctcgtgtt cgctaacaga
    13321 gaagacgcag aaccagtaaa aaagattact gttcccatcc ccagaacaaa tttgcgtcga
    13381 gtgaaggaca tctgattatc ccctaatgca gtggctgcaa ctgtccaccc tatccatgac
    13441 atctccctga aatttcaggg gttctgaaac tgctctacct cgaaccattc gcataagcca
    13501 taagccttgc cataggtcat aaatcgctcg ctaaacaagt acaacaccgc cgtcgatgac
    13561 tccacctgcc gtatatcggc atattcaggt gcaacatcca tcgcggcgat cgcggcttca
    13621 atctgcgctt cctggaagta atatggcgct tgcatcagca tggcgacttt gtaaggacga
    13681 ggatatgtct ggcactcaaa acgaacggcg tgtgcaatgg cgcgacaaat gtcctgctcc
    13741 acaacttgca aagacatcgc cgcgtagttt tcactcatgg cctgcgttga gtaatagtaa
    13801 tcatcctggc ttcccgtcat acaagcgata tccgcgtagt ctccgttctg cttaatgctt
    13861 tccagcaaaa ctgacagctc ctcttctgca accgaatagg gtggcgagag aaacacggcg
    13921 cgcgccacca gctgcccagc cgcagaatgc tggcgaatga actctgccag gatttcagcc
    13981 ggttctggtt cggttaccgt atcaacgggg tgacgcatcc cctgtaacca ttgccgcttc
    14041 gcctcgtcaa cttcgctgac tttcgctaac ggcaattcat cccgatggtt catctcaccc
    14101 attccttaca caggcagtag attgtgtgct gccagatcgg cggcaatatc ttccagcccc
    14161 agacgctgca atgtttcgcg ggtcggacaa ccaagctgtg ggtcccagcc catttctttg
    14221 tagaacatcg tgagcgaagc atgcatatcg tcacgatcca ttttgtcagt accttcagta
    14281 aagaccggga tctgcggatc cttgtcgaat acccaggaac agataagatc gtgttcatta
    14341 cgcatatctt tggtttgcat cagttttacc gtgtaggcac gatgcaacgt aaagatacgc
    14401 tctgcagcta aatctaattt ttcctgagtc atctcttcgc cggtgatcgc tttgaagaat
    14461 ttggcttcaa gcgccagatc gccccggtaa ttacggcttt tcagcggcga aacggtcatt
    14521 ggccagaccc agttgcacag ggtgacggcg ttatgcaaac agacccgcaa cagcgaccac
    14581 tttgcatatt taatttttgc gtcattgatt ggcgtgtagt ttttggtttc atcgtaggca
    14641 tcttcagaac caaaaagctc tttcgccact tcacgctgca gtttcaatgg caaacccgaa
    14701 ccaataaaat tgatatgggt atgcgtcatg cagtcacggt tgaacataca gttaacaatg
    14761 gaaccgacct gcgctgacgc ttcattggca tggtgaaccg gatagccaaa cggcgaccag
    14821 agtttatttt ttgcgtagcc ccagtactct tcacccaaat tccagcgttc tgcgatggca
    14881 tatgaaccat cagccaggtg actcagctca cccacacgat gcgccagacg gtagtaaaaa
    14941 tctttaatga aattaacgtc acccgcttcc agttgatccc agtgaatttc cgcatactct
    15001 tcagctggca gaacacgctt gaacacccct ttgctgtaac aataagtaaa gtcgcgatgc
    15061 agctgcccgt agttacacca taggccgtag tcatcgaaca aattcagacc aaccaggtta
    15121 ccaatcacac gaccatcgtc tttatcttca aaatctttcg gcccgttcgg gaagatggtg
    15181 gtatggacaa agttagctac acaagtgtta ccacctgtgc tgggcacgcc aaactctttc
    15241 acccgaggaa tattcatttg ggtcatacaa cggatcgggc aagagtggca gccactcatt
    15301 tttactgtgt atttctctgc cgccggacct aagtcaaaaa cggatttata ggtacgaaag
    15361 ccgaccgtgt tctgattgcc tggcggaatt tcccccgttt caatcggacc accttcagcc
    15421 gcgccccaaa acagcccttt acgtgctgtc caacgtgact tgggatctga atactctgcc
    15481 cacgattgcg gcgtacttgg cacgacatgg ttgttattcg caccaataag ttcagtcatc
    15541 atgtaatcgt tgagacgctt catctcctga cgatcggcaa tgttgacccc tttcgtccct
    15601 tcaaccgcaa tcgctttcag gtttttcgaa cccataattg cgccagttcc cgcaccgccg
    15661 ctgtggttac ggctattcaa catgccagag agaggaacaa ggttttcccc agcctgacca
    15721 atagccgcca cacaggtttc cggactggtc aatcgacaaa tttcttccgt cgttgcgcgc
    15781 gtcccttttc cccataagaa atcggctttt tccaggctaa ctttgtcatc tttaatcttc
    15841 agccataccg gtgatttcgc cttcccttcg ataataatga cgtcgtatcc agcgaatttc
    15901 atttgcgctg caaaaaagcc acccatatgg gcatcgacaa ctaaatttcc tttggtaaaa
    15961 gtagaaagtg aggtgatatt tacgcgagaa ctacaggggg caccagatcc agttaatggg
    16021 ccggtagcaa agactaattt attcgcttca tcgaaaggtt tcgtgcctgg cggtacttcg
    16081 tcatacataa ttttgtagcc gaagcccatg ccaccgacaa aacttttaaa cttactggaa
    16141 tcttcgaggg taatatttcc tgtcgtgaga ttgactctta atatattacc tgtccaaccg
    16201 ttagccatga ttttttcctt tgcaagattc acacagtaat atctttccac tcgataattt
    16261 ttaacgcccc tgttgggcag gcgtttgcgc attcaccgca taacacacat ttcgaggatt
    16321 ttttactttc ggtatttacg gtggccatca tccacgggca tgccgtagta caggcgctac
    16381 agccaataca gcgtttatga tcgacggtaa tacagccttc tttctgctgc caggtaatcg
    16441 caccaatcgg gcagacgttc atgcattgcg gctctttgca ttgacggcag gtgtccgcgg
    16501 tatagttgag atcgccatac aggccgccgc cagagccaac cccgttgtcg ccaaagaaat
    16561 aattgcggtg gattttgata cgggagaaga atgtccctac tgagccatcg ttgaagttgg
    16621 tacaagagat ttcacagcgg tggcaacccg tgcatcgcgc tcgctgggtc accagcaccc
    16681 ctttgggtgt atttatcaac cctactgtgc cgctatcaat atcttcctgt ttgcaaccca
    16741 aaagtgacag taacgcaggg gcaattgtta gaccagcaag gccttttcct gatatacgca
    16801 agaattctaa tcgcgttaag ccaatatcta atagtggacg atcaaccggg ttcatttatt
    16861 tcgcatcctc ttcgcaaaca cagtgctgac aattcagcag cctgatatta agcattcaac
    16921 taatacgctg ctgactctgt ccccaacacc agatattttc cctaacccga tagtgtatga
    16981 gaaaagattt aacgccgata aactaatcct ttatggttag gagaaa
//
LOCUS       DAZFRJ010000093        16998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000093 DAZFRJ010000000
VERSION     DAZFRJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.093"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            111..713
                     /gene="ais"
                     /locus_tag="ACHKUN_002786"
     CDS             111..713
                     /gene="ais"
                     /locus_tag="ACHKUN_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO6661015.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(752..1177)
                     /gene="nudI"
                     /locus_tag="ACHKUN_002787"
     CDS             complement(752..1177)
                     /gene="nudI"
                     /locus_tag="ACHKUN_002787"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO6661016.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1456..1998
                     /gene="yfaZ"
                     /locus_tag="ACHKUN_002788"
     CDS             1456..1998
                     /gene="yfaZ"
                     /locus_tag="ACHKUN_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO6661017.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2098..3300
                     /gene="yfaY"
                     /locus_tag="ACHKUN_002789"
     CDS             2098..3300
                     /gene="yfaY"
                     /locus_tag="ACHKUN_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO6661018.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3364..3825
                     /locus_tag="ACHKUN_002790"
                     /pseudo
     CDS             3364..3825
                     /locus_tag="ACHKUN_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3866..4768)
                     /gene="rpnE"
                     /locus_tag="ACHKUN_002791"
     CDS             complement(3866..4768)
                     /gene="rpnE"
                     /locus_tag="ACHKUN_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO6661019.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4962..6152)
                     /gene="glpC"
                     /locus_tag="ACHKUN_002792"
     CDS             complement(4962..6152)
                     /gene="glpC"
                     /locus_tag="ACHKUN_002792"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO6661020.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6149..7408)
                     /gene="glpB"
                     /locus_tag="ACHKUN_002793"
     CDS             complement(6149..7408)
                     /gene="glpB"
                     /locus_tag="ACHKUN_002793"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO6661021.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7398..9026)
                     /gene="glpA"
                     /locus_tag="ACHKUN_002794"
     CDS             complement(7398..9026)
                     /gene="glpA"
                     /locus_tag="ACHKUN_002794"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO6661022.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9299..10657
                     /gene="glpT"
                     /locus_tag="ACHKUN_002795"
     CDS             9299..10657
                     /gene="glpT"
                     /locus_tag="ACHKUN_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO6661023.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10662..11738
                     /gene="glpQ"
                     /locus_tag="ACHKUN_002796"
     CDS             10662..11738
                     /gene="glpQ"
                     /locus_tag="ACHKUN_002796"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6661024.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11780..12019)
                     /locus_tag="ACHKUN_002797"
                     /pseudo
     CDS             complement(11780..12019)
                     /locus_tag="ACHKUN_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12017..12121
                     /locus_tag="ACHKUN_002798"
                     /pseudo
     CDS             12017..12121
                     /locus_tag="ACHKUN_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12201..12851
                     /gene="inaA"
                     /locus_tag="ACHKUN_002799"
     CDS             12201..12851
                     /gene="inaA"
                     /locus_tag="ACHKUN_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO6661025.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12905..13159)
                     /gene="yfaE"
                     /locus_tag="ACHKUN_002800"
     CDS             complement(12905..13159)
                     /gene="yfaE"
                     /locus_tag="ACHKUN_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO6661026.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13159..14289)
                     /gene="nrdB"
                     /locus_tag="ACHKUN_002801"
     CDS             complement(13159..14289)
                     /gene="nrdB"
                     /locus_tag="ACHKUN_002801"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6661027.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14378..16663)
                     /gene="nrdA"
                     /locus_tag="ACHKUN_002802"
     CDS             complement(14378..16663)
                     /gene="nrdA"
                     /locus_tag="ACHKUN_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6661028.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16791..16934
                     /gene="ypaB"
                     /locus_tag="ACHKUN_002803"
     CDS             16791..16934
                     /gene="ypaB"
                     /locus_tag="ACHKUN_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO6661029.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4003 a         4169 c         4553 g         4273 t
ORIGIN      
        1 tgccgacatc gtaggatttt taatattaca ccaactgcta attatcgcca gaaatgtcgc
       61 tcaattttac ggtaattgtc tggttgcgct tgtctatagg tggagtttac gtgttagctt
      121 tttgccgctc atcattgaag tcaaaaaaat atttcatcat tttactggcg ctcgctgcaa
      181 ttgccggact gggtactcat gccgcctgga gtagcaatgg tttgccacgt atcgacaata
      241 aaacattggc cagactggca cagcagcacc cggttgtcgt tttgtttcgc catgctgaac
      301 gttgcgatcg ttcaaccaat caatgcttgt cagataaaac aggtattacg gttaaaggta
      361 cccaggatgc ccgtgaactg ggcaacgctt ttagcgctga catccctgat ttcgatcttt
      421 attccagtaa taccgtccgg accattcagt cggcgacctg gttttccgcg ggtaaaaagc
      481 tgacggtaga taaacgactt cttcagtgcg gtaatgagat ttatagtgca attaaggact
      541 tacaaagcaa agcgcctgat aaaaatatcg ttattttcac ccataatcat tgcctgacat
      601 atattgctaa aaataagcgt gatgcgacat ttaaacctga ttatctggat ggtttagtca
      661 tgcatgtgga aaaaggcaaa gtttatctgg atggggaatt cgttaaccac taaattttca
      721 atctgacagc cagtgatggc tgtcatcatt gttaaagaag acctttcaaa cgtaacgttt
      781 ttcgggtggc aacatttaaa tcataatgca ccagatcttc aggttttacc cacgcgtagt
      841 cctgaaactc ttcgtttatt ttcacttctc ggttggcaga aacgcagtca aaaatcaggt
      901 aaatcatata aatctcttcc ttgcgaccat ctgcatacgt cttggtgcga atatcatcgc
      961 tgaaggtcca cggcgtgatt tctgtcaaaa gcagctgttc tcccagttct tcgcgaattt
     1021 cgcggcgtag tgcctcttca attcgttcgc cagactccac gccgccaccc gaaagcgccc
     1081 attgaccggg gaaaacgccg cgatcgtcgg ccattttaca cagcaaataa gcaccatcat
     1141 tttgaatcaa agggcataca atagtccgtt gtcgcatagg ggtattcctt ataaataaaa
     1201 agaaaccatg agaatacggt aactcagaaa tatgtgaaag tgacagtaat ctcttttgca
     1261 atgataactg cacttattat tatttttgtg tgtatcggta aagtgtgttt attttttcgg
     1321 cgtttaatta tttaagaagt atttagttac aataagtgac tagatccctt ttttcgctca
     1381 ttgaatcctc tgtgccaggc agtacaatcc cgcagctttg ggaattttga ttaattgctt
     1441 gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc tggccggaat gctgttggtt
     1501 tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg gtaaagaata caccaatatt
     1561 ggtgtcggtt ttgggactga atcgacgggc ctggctttaa gcggtaactg gacacataac
     1621 gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga atctgcctct cgggccgtta
     1681 atggcgaccg ttggcggaaa aggcgtttac accaacccga attacggcga tgaaggttat
     1741 gccgcagcgg taggaggtgg tttgcagtgg aaaattggca acagcttccg tttgtttggc
     1801 gagtattact actctccgga ttcgctctcc agcggtattc aaagttatga ggaagcgaat
     1861 gctggcgcgc gttacaccat tatgcgtcca gtcagtattg aggcgggtta tcgctacctg
     1921 aatctgtcgg gtaaagacgg taaccgcgac aacgccgtgg ctgacggccc gtatgttggg
     1981 gttaacgcca gtttctgatc ccttccggcg cggcattctg tcgcgccatt cagtttctta
     2041 ctacgcctta tctccctttg cgttatagtg attcgataac aaaagcagga gataaccatg
     2101 ttaaaagtgg aaatgctatc caccggggat gaagtgttac acgggcaaat cgttgacact
     2161 aacgctgcct ggctggccga ttttttcttt catcaggggc tgccattatc tcgccgcaat
     2221 acggtggggg ataaccttga tgacttagtc accattcttc gcgaacgtag tcagcacgcc
     2281 gatgtgctga tcgttaacgg cgggctggga ccgaccagcg atgatttaag cgcactcgcc
     2341 gctgcgacag caaaaggtga aggcatggtg ctgcatgaag cctggctcaa agagatggaa
     2401 cgctatttcc acgaacgtgg acgagtaatg gcaccgagca accgtaaaca agcggagctg
     2461 cctgccagtg ctgaatttat caataacccg gtaggcaccg cctgtggttt tgccgtgcag
     2521 cttaatcgtt gcctgatgtt ctttactccc ggcgtaccgt cagaatttaa ggtgatggtc
     2581 gagcacgaaa tcctgccgcg tctgcgcgag cgtttttctt taccacagcc gccggtttgt
     2641 ctgcgtttaa ctacctttgg tcgttcggaa agcgatctgg cacaaagcct ggacactcta
     2701 caactgccgc cgggcgtaac aatgggctat cgctcttcaa tgccgattat cgaactgaaa
     2761 ctcaccggac cggcaagcga gcaacaggcg atggaaaaac tgtggctgga cgttaaacgt
     2821 gttgccggac agagcgtgat tttcgaaggc accgaaggac tgcccgcgca gatcagtcgc
     2881 gaattgcaaa accgccagtt cagcctgacg ttgagcgagc aattcaccgg tggtttattg
     2941 gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt tccttcacag
     3001 gaagaaaccc tggcgcaaac tgcgcactgg attacagaac ggcgggccaa ccattttgcc
     3061 gggctggcac tggctgtttc gggtttcgag aacgaacatc tcaactttgc gctagccaca
     3121 ccagacggca ctttcgctct gcgtgtgcgt ttcagcacta cgcgctacag cctggctatc
     3181 cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc gccgttggtt aaacggccag
     3241 gatatcgcca gtgagcatgg ctggattgag gttattgagt ccatgacctt atctgtctga
     3301 ttccgccact ctgtgccggt ttactgcacc ggcacattcc ccctccggca aaacttgtcc
     3361 aaccctccct acggtgagcg tggtgttggg gccagtgtgg cacgggctgc gcgctgggga
     3421 cgcattgaga attacatggc gcaagtcaac gattcgcttt gtctgttagt gcaggtggaa
     3481 agtaaaacgg cactggataa cctggacgag atcctcgacg tcgaagggat tgatggcgtg
     3541 tttattggac cagcggatct ttctgcgtcg ttgggctacc cggataacgc cgggcacccg
     3601 gaagtgcagc gaattattga aaccagcatt cggcggatcc gtgctgcggg taaagcggct
     3661 ggttttctgg ctgtggctcc tgatatggcg cagcaatgcc tggcgtgggg agcgaacttt
     3721 gtcgctgttg gcgttgacac gatgctctac agcgatgccc tggatcaacg actggcgatg
     3781 tttaaatcag gcaaaaatgg gccacgcata aaaggtagtt attgatatca aaggcccatg
     3841 gggatcggct gtgggcctgt gttaattagt ggttattcgc tgccagatcg gcttcgctta
     3901 gctgggtggc cgcgagcacc tggtcacgat caatgccctg ttccagcatg cgcaaagcaa
     3961 ttttaatggc ttgttcatgc ataccttcct gccagccaat ttgatgccct tcctgccgta
     4021 gccgttcagc aatagtcatt aatctctcct tttgtagtgg tgaacgttcg gcaatctcct
     4081 caataaaacg ggtgaaacgg gaggtatcgc cgcattgcag cagataatta aacagtgttt
     4141 gtagctggct gtcattagtg aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatgc
     4201 cgattaaatc gcggtcgcga atatgctttt gaatcagttc cagcagagct atacgccgat
     4261 gttgcatgat ctcatcgtca ggtacgatgg tgatatccac caacggaaaa gcttcggtgt
     4321 acaactgccg ggcaagttgc ggatcgtcaa actcatccag ccagttaagt gagtacgggt
     4381 agggcgatgt ttcgccatga taaaacagca atggcaccac cagcggaact ctgtcatagc
     4441 ctttatccag gtgacgctgc atggcggcag tggcatagcg cattagccga aaagccatat
     4501 tcttttctgc agagctttga tgttcaatca cgcagtagat atagccgtca ccgtcgctgg
     4561 tttccacgga ccacaaaaca tccgagtagt aagcgcgtaa acttttttca ataaaactag
     4621 tgggttccag gcgtaaggtt tgcaggttgc aaagcttgcg cagtggttct ggtaaatgta
     4681 tttcgagaaa atcccgtgcg gtttcgggtg tgaacataaa ggttttaaac accgcatcat
     4741 gcggggagga tgttgttgat tcggtcatgg ttttccgtca cctgatattt atgatgacgc
     4801 gacattacgt ctatcccggg cctttgacat ctgtcagttt cagcctttgc gagacatttt
     4861 cagaggaatt ttcaaagcgt tgcaacgcat acatggtgcg tggaatcgcg ctcgcaaaaa
     4921 tgcccgataa gcaatgtgct taccgggcat caaaaggtcg gttaagccag cgcctgggcc
     4981 agtagcgtaa tcggatgttc gcagcgaagg ctcgtggaca tctcaatctg ccatttgcag
     5041 gtttcgcagt cggtgatgac cagatccgcg ccgctctctt ctatctggcg gaacagtggc
     5101 gcaccgatgg cttgtgaggt ggggtagttc tcttttttga aaccgtaagt acccgcaata
     5161 ccgcagcact gggaatccag caccgttaac tcaagccccg ggattttacg caacagctcc
     5221 agggtgtaga gcgtccagcc cattttttcc atatggcacg gagtgtgata aaccactttc
     5281 agcggcagcg gtttcagcgg taacgttttg ccttcgtcca gcttgcgcca cagccagcgg
     5341 gttgccagtt cgatatgatc gcgcaagcct ttgttgtcga cattcagcac ttccgggtat
     5401 tcgtcgcgca gggcaaatgt acaggttgag gaggtggcaa tcaccggaat gccttttact
     5461 cccacagctt cgcggatcga ctctacattc gtaattgcct gtttgcgtgc tttatcggta
     5521 aagccgttgg cgattagcgg tacgccgcag catttttctt tgctgagcag ttgtacaccg
     5581 gtacccattg cgttgagcac tttaattaaa tctttaccta actgcggatg gttgtagtta
     5641 acgaagcagc cgtgaaagaa agcgacctgg tctttatatt gtgcttgctg agccgctatg
     5701 ctgcgatacc agcgacgaaa cgtaccgaag gagtatttcg gtagcgtgcg gcgatgatcg
     5761 atttttaacg ccgcatcaag cagctgccgc accggtttca gcgaggtagc ggtgttgacg
     5821 attggtgcga acggcgtgga aacgctaccc atcaggtcgg tatgactcaa cacaaaatta
     5881 cgcagcgacg ggcgcgtggt gtcatatttt gcccgcgcgc gctggataat atcgccaatc
     5941 ttcacatcgg acgggcaggc gacttcacaa cgtttgcagt tgatgcaata tttcagcgcc
     6001 tcgtcataca gtgcgccatc tttcaaacgc agacgctcgc catccggccc ggcttgtttt
     6061 ggccctggat aaccgggatt cacccggctc accgggcagg cggtggtgca gacggtgcac
     6121 ttaatgcagt tttcgaagct ggtgtcgttc attgttggcc tcctgcgcgt tgggcaatct
     6181 gttgagcggc atgtaaagca ctgacggcac aaacaccgcc gccgcatccc tgggcgatgg
     6241 gatcaaatcc gcccagcacc gaaccgatgg caaacaggtt ctcaatggtt tgccctgcct
     6301 gcgacgggcg tagcgtctga tcagtggtta caccgaactg ctgccacggt tgcggcgcaa
     6361 aaaaatcgcc cttataccat tcaccccgcg tggcggtttg tagcacatca aggccgagaa
     6421 tcggctcgcg aatgccgtta cgttccgcta ccagtccgcc actaaagaaa ctgccgctgg
     6481 cgagaaccgc gaaacgtgga cgtagcggaa tatcggcgtg attgcgggtc cagatttcat
     6541 tcactacgcc atttttacag gtcacttttt tcacttcatc gcccggcatc cacacgccac
     6601 cctggcgcac aaactggcgc tgtaactggt tttgcagacg aatgcccagc acggaaggcg
     6661 gcagcgttgg caaaagcatc agtgaacaag gtagtttttc attcaaccaa cgccacagtt
     6721 tgtcatcggc caggccgaag caggcgggca tcaggatcat ttcgcaggta ttggcgacag
     6781 gaataagcgc atcaagtaac agcggccagt tttcttcatt atcaaggaaa cgggcgatat
     6841 tcaccgcgcg aaattcggtg gcgttattgc gcaggacatc cagttccggc agctctattt
     6901 ctgcggtttc aaccgaaaga tccagttcgc gcaacgaagc cgccgcaaga tgcgcctgga
     6961 aatccatcag cccgcttatc cccactacac atatcttctt cgcgggtagc ggccagacgg
     7021 gaacttctgg cgaacttaac caggttgcac gcaaagtgcc gagcggggta acgcgctgat
     7081 gggccagttc cacgctgccc tgcaattgcg caccgctttc agcgatcaat gcctgcgcct
     7141 ggcaagcgag atcgagcacg cgttgtggtt cgagaagact gtaaggatgg gctggtgcct
     7201 gctgacgcaa agattccagt ccactgtgaa tgtctgtcac cggttgacca tcaggcagat
     7261 ggctcagcaa atccagcgaa ccggatgaga aatgcagcgc gctttgacca cgcgtgacaa
     7321 tggcacagcg caggccgtgt ttttgcagtt gcaggccaca gagtaatccg gcgaggccgc
     7381 cgcccataat gacagtatca aagcgcatct ttctgctcct tctccagacc acacaatccc
     7441 tgataaaccc agcgggtaaa ttcgctttcg cgcagtgcat ctccccaggc gatgggttgc
     7501 acgcctttcc agcgttcgtt aaggaaggtg gaaagttgct cgatagattg cgcggacgtc
     7561 gtgacgttaa aacgttgcag cagtccggca gcgcggcagg cgcagagttc gccctggcag
     7621 gtacccatcc ccacacgggt acgacggcgt aaatccagca ggctattaac gtttaaattt
     7681 tctaccgcgt actgaacttc acccgcagtt accgcttcgc actcacatac caggctacga
     7741 tgcagacggc cttcgctcag ccaggcaggc gtgcgatcgc catgacgata aaccgcagaa
     7801 ccgcgcagcg gggcaggcag ggagatgact ttacgcaagg taacttcagc gggttcttgt
     7861 gaaccaggca gtgccagatc ggcagtcgta caggggcgcg tgttgcccag tttgcggcat
     7921 accgcgtcgg tagcccattc agccatcagc cgataggtca tcagtttgcc accggtgatg
     7981 gtgataaatc cgtccagacc atcgcgttca gcatggtcga gcagcacgat gccacggctg
     8041 acgttacgtc cgctcgggtc gtcatcgctg gcaaccagcg ggcgcacgcc agaataggcc
     8101 cgcaaaatgc gcgttttcgc catcacgggg gccagttttt ccccttcacg cagcagaata
     8161 tcaacctctt ctgccgtcac tcgattatcg tcaatctcgt tgtaatcaat acgtaaagag
     8221 gtggtgccaa tcagcgaaat ggtatcgcca ggcaccagaa tatcggcgtc ggaaggttta
     8281 cggcagcggt tgatcacatg ctggttaatg cggtgatcca tgatcagcag cgatcctttc
     8341 gccgggaaca tgcgaatgcg cagatcggca tattcggcaa tgtgttgccc ccagatccca
     8401 gcggcattaa ccacgacagg tgcatgaagg gcctgagttt cgccggtgag atggttacgt
     8461 acacgaacac cgcacaccgt cgcgccttca cgaatcagcc ccgtgacttc atgagcggta
     8521 aggataacgg caccgtgttc tttggcatcc agcatgtttg ctgcggtcag acgaaatgga
     8581 tcaacggtgc catccggaac tttcaccgcg ccaatcagtg ccgggttaac ggcaggttcg
     8641 ataatgcgcg cttgctgcgg gtctatagct tctgcgctga tccctgcttc ttcgcaggcg
     8701 cgaataaaag tggcctggaa tgagaggtca tcttccggca gggtgataaa caggccgttg
     8761 gttggttcaa cgcagtgacg tgcaatgcgt ttcaggatct ggttttcact aatgcattcg
     8821 cgggccgatt ccgcatcggt taccgcatag cgcgcaccgc tgtgcagcag gccgtggtta
     8881 cgcccggtgg caccggttgc gatgtcgtgg cgctcaacca aaatcacgcg cagcccgcgc
     8941 agggcacagt cgcgggcaat cccggctccc gttgcgccgc caccaatgat aatcacgtca
     9001 cttgattgcg agtcgcgagt tttcattgtt atccctctga agttcgtttt ttaccattta
     9061 gccatagtaa aaacatgaat tgtttgattt cgcgcatatt cgctcataat tcgaaagtga
     9121 aacgtgattt catgcgtcat tctgaacatt ttgtaaatct tatttaataa tgtgtgcggc
     9181 aattcacatt taatttctga atgttttctt aacatcgcgg caactaaaga aacggcaggt
     9241 tctcccactg aatcagtctg ttaatcataa ataagaccac gggccacgga ggctttcaat
     9301 gttgagtatt tttaaaccag cgccacacaa agcgcgctta cctgctgcgg agatcgatcc
     9361 gacttatcgt cgattgcgct ggcaaatttt cctggggata ttctttggct atgcggctta
     9421 ctatttggtt cgtaagaact ttgcgcttgc tatgccttat ctggtcgagc agggattctc
     9481 tcgcggtgat ttaggtttcg cgctttcggg gatctcgatc gcttatggat tttcgaaatt
     9541 cataatgggt tcggtatcgg atcgctcgaa tccgcgcgtt ttcctgcccg caggtttgat
     9601 tctggcggcg gcagtgatgt tgtttatggg ctttgtgcca tgggcgacgt cgagcattgc
     9661 ggtgatgttt gtactgttgt tcctctgcgg ttggttccag gggatggggt ggccgccgtg
     9721 tggtcgtact atggtgcact ggtggtcgca gaaagaacgt ggcggcattg tgtcagtgtg
     9781 gaactgtgcg cacaacgtcg gtggtggtat tccgccgctg ctgttcctgc tggggatggc
     9841 ctggttcaat gactggcatg cggcgctcta tatgcctgct ttctgcgcca ttctggtggc
     9901 attattcgcc tttgcgatga tgcgcgatac cccgcaatcc tgtggcttgc cgccgatcga
     9961 agagtacaaa aatgattatc cggacgacta taacgaaaaa gcggaacagg agctgaccgc
    10021 gaagcaaatc ttcatgcagt acgtgctgcc gaacaaactg ctgtggtata tcgccatcgc
    10081 caacgtgttc gtttatctgc tgcgttacgg catcctcgac tggtcaccga cttatctgaa
    10141 agaggttaag catttcgcgc tggataaatc ctcctgggcc tacttccttt atgaatatgc
    10201 aggtattccg ggcactctgc tgtgcggctg gatgtcggat aaagtcttcc gtggcaaccg
    10261 tggggcaacc ggcgttttct ttatgacact ggtgaccatc gcgactatcg tttactggat
    10321 gaacccggca ggtaacccaa ccgtcgatat gatttgtatg attgttatcg gcttcctgat
    10381 ctacggtcct gtgatgctga tcggtctgca tgcgctggaa ctggcaccga aaaaagcggc
    10441 aggtacggca gcgggcttta ccgggctgtt tggttacctg ggcggttcgg tggcggcgag
    10501 cgcgattgtt ggctacaccg tggacttctt cggctgggat ggcggcttta tggtaatgat
    10561 tggcggcagc attctggcgg ttatcttgtt gattgttgtg atgattggcg aaaaacgtcg
    10621 ccatgaacaa ttactgcaaa aacgcaacgg aggctaatgg catgaaattg acgctgaaaa
    10681 accttagcat ggcgatcatg atgagcacta tagtcatggg aagcagtgca atggcggcgg
    10741 acagcaacga aaaaatagtc atcgcccatc gcggtgccag tggatatttg ccggagcata
    10801 cgctgccagc aaaagcgatg gcgtatgcgc agggagcgga ttatctggaa caggatttgg
    10861 tgatgaccaa agacgaccat ctggttgttc tgcatgacca ttatctcgat cgtgttactg
    10921 atgttgccga tcgtttcccg gaccgggcgc gcaaagacgg tcgttactac gcgatagatt
    10981 tcacgctgga tgaaattaag tcgctgaaat ttaccgaagg tttcgatatt gaaaacggta
    11041 aaaaagtgca gacttatccg gggcgtttcc caatgggtaa gtccgacttc cgggtgcaca
    11101 cctttgaaga agagattgaa tttgttcagg ggttaaatca ctctaccggg aaaaatatcg
    11161 gtatttatcc tgaaatcaaa gcgccgtggt tccatcatca ggaagggaag gatattgcgg
    11221 caaaaacgct ggaagtgctg aagaaatatg gttacaccgg taaggacgac aaagtttatt
    11281 tgcaatgttt tgatgctgat gagctgaagc gtattaagaa tgagctggaa cctaaaatgg
    11341 gcatggatct caatctggta cagttgattg cctataccga ctggaatgaa acgcagcaga
    11401 aacagccgga tggaagctgg gttaattaca actacgactg gatgtttaag ccgggtgcta
    11461 tgaaacaggt ggcggaatat gccgacggaa tcgggccgga ttaccatatg ttgattgagg
    11521 aaacatcgca gccgggtaat atcaaactca ctggcatggt gcaagatgct cagcagaata
    11581 aactggtagt gcatccttat accgtgcggt cagataaact gcctgaatac actactgatg
    11641 tgaatcagtt atatgatgct ctgtataaca aagcgggtgt aaatgggctg tttactgatt
    11701 tccctgataa ggcagtaaaa ttccttaata aagagtaatg ctactgcagg atggcgttca
    11761 tgcgccatcc tgtctcacat caaatttttc cattcaccac gagttgttcc gtacacgttt
    11821 gtagtaaaaa cagtggactg ccttctaccg gcttttcttt agccaacaac gaaatctgtc
    11881 gatagaaggt tggttcgtga ggaacggaac acaattcacc tgcaatgctt ttcagcgtta
    11941 actctggttg taaggcgatg cctaatccct gtcgaataaa cttcatcgct gtatccgact
    12001 gattaaattc acattttatc tactgggcgc actattagtg gtagtggctg gtggaatcta
    12061 tgcgctgcaa atgaagggta aaagcggtgt cacgctagta gtggcaaaag aaattcatta
    12121 atacgacacg tttcattaag attttcctca ggtaaccacc tatagtcatt gtcgttaata
    12181 aaaatgacag gcgatagggt atggcagttt cagcaaagta cgacgaattt aatcactggt
    12241 gggcaacaga gggcgactgg gttgaagagc ctaactatcg ccgtaatggt atgagcgggg
    12301 tgcaatgcgt cgagcgcaac ggcaaaaagc tgtatgtaaa gcgcatgacg catcatctgt
    12361 ttcattccgt acgttatccg ttcggccgac caacgattgt ccgtgaggtt gcggtaatta
    12421 aagaactgga acgggccggt gtcatcgtgc cgaagatcgt ttttggtgaa gcggtgaaaa
    12481 ttgagggtga atggcgagcg ttgctggtga ctgaagatat ggcggggttc atcagcattg
    12541 ctgactggta tgcccagcat gcagtatcgc cttattctga cgaagtacgg caagccatgt
    12601 tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcat taatcgtcag catggctgtt
    12661 gttatgttcg ccatatctat gtgaaaacag aaggtaatgc agaagctggt tttcttgacc
    12721 tggagaaaag tcgtcgccgc ttgcgtcgcg ataaagcgat taatcatgac ttccgccagc
    12781 tggaaaaata tctggagcca atccccaaag cggactggga gcaggtgaaa gcgtattact
    12841 acgccatgta atcgttgtag gccagataag acgcataagc gttgtatctg gcactacaac
    12901 caattcacat ctcgatttca atatcgcctt ttgcccggca acaacagggc aaaatttccc
    12961 ccggctgaat aaaggctaac ggttcggcaa tccagtcaac ctgacctgca accagacggg
    13021 tgcgacagga gccgcagtaa ccttcgcgac actgatactc aaccgccaca ttgtgggatt
    13081 ccagcgccgc cagaagggaa gggtgttcat cctggcacag cagttgtgtg ccagtgatgc
    13141 gcagggtaac gcgggccatc agagttggaa gttactcaaa tcgtcggtgt ccacttccgc
    13201 gtcaatctgc ccgaccagat aagaactgac ttccacttcc tgcggagcaa cctgcacgtt
    13261 atcagacacc agccaggtgt tgatccatgg gatcgggttg gagcgcgtct ggaacggcag
    13321 atccaaaccg actgcctgca tacggatatt ggtgatgtat tcaacgtact ggcagagaat
    13381 gtctttattc agaccaatca tcgaaccgtc gcggaacaga taatccgccc agtctttctc
    13441 ctgttgagct gcctgaacaa acaggtcata gcactcctgc ttacactctt cggcaatttc
    13501 cgccatctca ggatcgtccg cgccgctgcg cagcagattc agcatatgct gggtgccggt
    13561 caggtgcagg gcttcgtcgc gggcaatcag gcgaataatt ttggcgttgc cttccatcaa
    13621 ttcgcgttct gcaaatgcga aggaacaagc aaagctgacg tagaaacgaa tcgcttccag
    13681 cgcgttaacg ctcatcaggc agagatacag ttttttcttc agctcgcgca ggctaacggt
    13741 cacagtttta ccgttaacgg tgtgggtacc ttcgcccagc agatgccagt agctggtcat
    13801 ttcgatcagc tcatcgtaat agctggagat cccttccgca cgtttctgga tctgctcgtt
    13861 ggtgacgata tcgtcaaaca caacagacgg atcgttaacg atattacgaa tgatatgagt
    13921 ataggaacgg gaatgaatcg tttctgagaa cgcccaggtt tcgacccagg tctccagttc
    13981 cgggatcgag atcagcggca gcagtgccac gttcgggcta cggccctgaa tggaatccag
    14041 cagcgtctga tatttcaggt tgctgataaa gatgtgtttt tcgtgttcag gcagtgcctg
    14101 gtaatcgata cggtcgcgag aaacgtcaac ttcttccgga cgccagaaga aagagagctg
    14161 cttttcgatc agcttttcga agatgtcata tttttgctga tcgtagcgag ccacgttgac
    14221 cggctgacca aagaacatcg gttctttgag ctgatcattt ttcgtctgtg aaaaggtggt
    14281 atatgccatg agtgtgtcct gtggttttat tacccctcac cctaaccctc tcccgcaagg
    14341 ggagagggga cggtttggtg caaaggctgg gggattatca gatcttacat gcgccgcttt
    14401 cgcagccatc gtcctggatt gacggtacca gatcgtcctg cgcgtcttca gcgccgtcac
    14461 gggtgttctg ataatacagc gttttgaccc cgaatttgta ggcggtgagc aggtctttca
    14521 gcaactgctg catcggcact tttcctgacg ggaaacgtga cggatcgtag ttggtgttag
    14581 cagagatcga ctgatcgata aatttctgca tgatacctac cagttgcagg tagccatcgt
    14641 tacccggcat ttcccacagc agctcatagg cgtcgtgcag gtgctcgtag tccggcacca
    14701 cctggcgcaa aataccgtct ttcgacgctt tgatgctgac gtaaccgcgc ggcggttcaa
    14761 taccgttagt ggcgttagag atctgtgaag aagtctctga cggcatcagg gcagaaagcg
    14821 tggagttacg caggccgtgc gttttgattg attcacgcaa tgcttcccag tcgtaatgca
    14881 gcggctcatt agcgatggta tccagatctt tcttataggt atcgatcggc aggatccctt
    14941 tcgcgtaagt ggtttcgtta aaccacgggc acgcgccttg ctcttttgcc agctcgttag
    15001 acgctttcag caggtaatac tgaatcgctt cgaaggtttt atgcgtcagg ttgttggcgc
    15061 tgccgtcgga gtaacgcaca ccgtgcttcg ccaggtagta agcgaagttg atcacaccaa
    15121 tacccagcgt acgacgaccc atcgctccac gtttggcggc cgggatcggg taatcctgat
    15181 aatccagcag cgcgtcaagt gcacgaaccg ccagaattgc cagctcttcc agttcatcca
    15241 ggctattaat tgcgcccagg ttgaaagcag acagcgtaca cagcgcgatt tcaccgttct
    15301 cgtcgttgac gtcgttcagc ggtttggtcg gcagggctat ctccaggcac aggttagact
    15361 gacgcactgg cgcgatggcc ggatcaaacg ggctatgggt attgcagtgg tcaacgttct
    15421 gaatatagat acgaccggta gacgcacgtt cctgcatcat cagcgagaac agctcaacgg
    15481 ctttcacacg ctgcttgcgg atgctgtcgt ctttctcata tttggtatac aggcgttcaa
    15541 actcttcctg atcggcgaag aatgcgtcgt acagccccgg tacgtcggac gggctgaaca
    15601 gggtgatatc ttcacctttc agcaggcggg tatacatcag tttgttgatc tgtaccccgt
    15661 agtccatatg gcgcacgcgg ttgccttcca caccacggtt gtttttcaac accagcaggc
    15721 tttccacttc cagatgccac atcgggtaga acagcgttgc cgcaccgccg cgcacgccgc
    15781 cctgagagca ggatttcacc gctgtctgga aatgtttgta gaacggaata cagccggtat
    15841 ggaacgcttc accaccgcga atcgggctac ccagcgcacg aatacgcccg gcgttgatgc
    15901 cgatcccggc acgctgggaa acgtatttca caatcgcgct ggaggtagcg ttgatggaat
    15961 ccagactgtc accgcactcg atcagtacgc aggagctgaa ctgacgagtc ggggtacgca
    16021 cgccggacat gattggcgtc ggcagcgaaa ttttaaatgt ggaaaccgcg tcgtaaaaac
    16081 gcttcacata ttgcaggcgc gtttcacgcg ggtagttcga gaacaggcac gcggcaacca
    16141 gaatataaag gaactgggcg ctttcgtaga tttcgccggt cacgcggttc tgtaccagat
    16201 atttgccttc cagctgctta acggcagcat aagagaaggt catatcacgg tcgtgatcga
    16261 taaacgtgtc catctgcttg aactcttctt ccgtgtagtc ttccagcaga tgattatcgt
    16321 atttgcccat ctcgaccatt ttcaccacgt ggtcgtacag cgcaggcggc tcaaactggc
    16381 cgtaggcttt tttacgcagg tggaagatcg ccaggcgcgc ggcgagatac tgataatccg
    16441 gcgcatcacg ggagatcagg tctgcggccg ccttgataat ggtttcgtgg atgtcagagg
    16501 tcttgatacc gtcataaaac tgaatgtggg agcgcagctc gacctgggaa atcgaaacgt
    16561 tatgcagtcc ttctgccgcc caatccagaa cgcgatggat tttgtcgaga ttgatgcgct
    16621 ctgtgctacc gtcgcgcttt gtcaccagca gattctgatt catgtatgtc gtacctgttt
    16681 ttggaattct ttccgcaata gtttcatgca gactacctgt agtacgggaa gctgcttaga
    16741 acactatata tagggggtat gtttgggatt cactgcaaga tagtgtgaaa atggccctct
    16801 tgcaagtgca taactttgtg gataactcag gaaggaaaaa gtggctttcg cgcaccttag
    16861 gtcagacaag gtgtccggga aagtcaatgg gaagagaaaa atttgttaaa aataactgtt
    16921 cggcgatttc ttgaacggca gaataggttg gataaattga tatagatggt ttattaatct
    16981 ttttgctaat gattgcaa
//
LOCUS       DAZFRJ010000094        16796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000094 DAZFRJ010000000
VERSION     DAZFRJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.094"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(81..2072)
                     /gene="tkt"
                     /locus_tag="ACHKUN_002804"
     CDS             complement(81..2072)
                     /gene="tkt"
                     /locus_tag="ACHKUN_002804"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6661030.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2350..3108
                     /gene="loiP"
                     /locus_tag="ACHKUN_002805"
                     /gene_synonym="yggG"
     CDS             2350..3108
                     /gene="loiP"
                     /locus_tag="ACHKUN_002805"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO6661031.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3164..3907)
                     /locus_tag="ACHKUN_002806"
     CDS             complement(3164..3907)
                     /locus_tag="ACHKUN_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6661032.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3894..5003)
                     /gene="dgaE"
                     /locus_tag="ACHKUN_002807"
     CDS             complement(3894..5003)
                     /gene="dgaE"
                     /locus_tag="ACHKUN_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO6661033.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5007..5867)
                     /locus_tag="ACHKUN_002808"
     CDS             complement(5007..5867)
                     /locus_tag="ACHKUN_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO6661034.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5864..6613)
                     /locus_tag="ACHKUN_002809"
     CDS             complement(5864..6613)
                     /locus_tag="ACHKUN_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO6661035.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6639..7124)
                     /locus_tag="ACHKUN_002810"
     CDS             complement(6639..7124)
                     /locus_tag="ACHKUN_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO6661036.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7135..7563)
                     /locus_tag="ACHKUN_002811"
     CDS             complement(7135..7563)
                     /locus_tag="ACHKUN_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6661037.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7682..10480)
                     /gene="dagR"
                     /locus_tag="ACHKUN_002812"
     CDS             complement(7682..10480)
                     /gene="dagR"
                     /locus_tag="ACHKUN_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO6661038.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10739..11659)
                     /gene="speB"
                     /locus_tag="ACHKUN_002813"
     CDS             complement(10739..11659)
                     /gene="speB"
                     /locus_tag="ACHKUN_002813"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO6661039.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11795..12526)
                     /locus_tag="ACHKUN_002814"
     CDS             complement(11795..12526)
                     /locus_tag="ACHKUN_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661040.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(12672..14648)
                     /gene="speA"
                     /locus_tag="ACHKUN_002815"
     CDS             complement(12672..14648)
                     /gene="speA"
                     /locus_tag="ACHKUN_002815"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO6661041.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14657..14788)
                     /gene="yqgB"
                     /locus_tag="ACHKUN_002816"
     CDS             complement(14657..14788)
                     /gene="yqgB"
                     /locus_tag="ACHKUN_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO6661042.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(14882..15025)
                     /gene="yqgG"
                     /locus_tag="ACHKUN_002817"
     CDS             complement(14882..15025)
                     /gene="yqgG"
                     /locus_tag="ACHKUN_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO6661043.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(15136..15387)
                     /gene="yqgD"
                     /locus_tag="ACHKUN_002818"
     CDS             complement(15136..15387)
                     /gene="yqgD"
                     /locus_tag="ACHKUN_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO6661044.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            15443..16597
                     /gene="metK"
                     /locus_tag="ACHKUN_002819"
     CDS             15443..16597
                     /gene="metK"
                     /locus_tag="ACHKUN_002819"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO6661045.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         4106 a         4652 c         4208 g         3830 t
ORIGIN      
        1 tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg ccgaagcgac
       61 ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacaac gttatcaaca
      121 gtgaagccga actcttcaaa cagcagctct gccggagcag attcaccgaa ggtggtcata
      181 ccgacgatag caccgttcag gccaacatac ttgtaccagt agtcagcaat acccgcttct
      241 acagcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc agcatcctgc
      301 ttgtcaaatg cgtcggtaga cggcatggac accacgcgcg ctttcacgcc ttcggcagtc
      361 agtttttcgt aggcagcaac agccagttca acttctgaac cggtagcgat gaaaatcagt
      421 tccggctgac cggcgcagtc tttcagcaca taaccaccgc gcgcgatgtt tgccagttgc
      481 tcttcagttc gttcctgctg cgccaggttc tgacgggaga ggatcagtgc ggtcgggccg
      541 tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc acacggacgc
      601 catgtagaca tgttcggggt tacgcgcaga gaagcgacct gctctaccgg ctggtgagtc
      661 gggccatctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg acgctgtttc
      721 atcagcgcag ccatacgtac ggcgttacgt gcgtattcca cgaacatcag gaaggtggag
      781 gtgtacggca ggaaaccacc gtgcagggag ataccgttag caatcgcggt cataccgaac
      841 tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc tttagaacca
      901 gaccacaggg tcaggttaga cggtgccagg tcagcagaac cgccgaggaa ttccggcaac
      961 agcggaccga aagcttcgat agcattctga gacgctttac ggctggcgat tttcgccggg
     1021 ttagcctgca gtttagcaat gaactctttt gctttcgcgt cgaagtcaga cggcatttcg
     1081 cctttcatac ggcgggtaaa ttcagcggct tcctgcggat aagctttcgc gtaagcagcg
     1141 aatttctcgt tccatgcgga ttctttcgcc tggcctgctt ctttcgcatc ccactgagca
     1201 tagatttcag acgggatttc gaacggtgca tatttccagc ccagctgttc gcgggtcagg
     1261 gcaatttcag cgtcgcccag cggcgcaccg tgggagtcgt gagtaccggc tttgttcggg
     1321 gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt cactgcgcgc
     1381 gcttcttcta ctgcgcgttt gatggatgcc gcgtcatgac cgtcgatgtc gcgaataacg
     1441 tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc ttcaacgtga
     1501 ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag cttcagcgta
     1561 cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc gcccatgaag
     1621 gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc cgccagcgtt
     1681 ttttctgcaa tcgccatacc tactgcgttg gcaataccct gccccagcgg accggtggtg
     1741 gtttccacac cagcggtgta acccacttcc gggtggcccg gagttttaga gtgcagctga
     1801 cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag caggctgtag
     1861 atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc ccaggacgga
     1921 ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc agccataccc
     1981 ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct cagcgcacga
     2041 atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat gatgaagggc
     2101 acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt tgccaacaat
     2161 ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag aaatctctgg
     2221 atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc ttatactgag
     2281 actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac cttgtgcgga
     2341 agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac ggtactgact
     2401 ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc ttttcaggct
     2461 tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca ggagatggac
     2521 agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac aactattgcc
     2581 aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta tatggcgaag
     2641 gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg gctgatggat
     2701 atgatgacgg ataacgaagt cgaagcggtg atcggtcacg aaatggggca cgtggcgtta
     2761 ggccatgtga aaaaaggaat gcaggtggca cttggtacaa atgccgtgcg agtagctgcg
     2821 gcctctgcgg gcgggattgt cggaagttta tctcaatcac aacttggtga tctgggcgag
     2881 aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga ttattcttac
     2941 gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt tgaaaaactg
     3001 gcaaaactgg aagaaggtcg ccaaagctca atgtttgacg accatcctgc atccgccgaa
     3061 cgcgcccagc atattcgcga tcgtatgagc gcggatgggg ttaagtaagt cagacatacc
     3121 gatctacaac ataattgtag gtcggtaacg cctgaatccc cgatcagagt agtgatttca
     3181 ctttatttag caaaacggct acatcatccg gtcgggtata tcccgtatca gggtctataa
     3241 tcgagctgta aacatgtggc ataatgcgcg gcacccccgc ctccagacaa gttttcagaa
     3301 tcacactgaa gttatccaaa tcaatcccgc cagtcggctc aatcagcgtc ataccattgc
     3361 gcgccgccgt ggtcgccagc gcgtatagct ccggaagcga cctctcgccg cccatcggaa
     3421 agaacttcgc cgcgtgtgcg cccatatcta gcatcattcg caccgccgta tcgcaggaga
     3481 cgcgggcagg cgtaccctgg ctgctgctaa cgccggtgga aatcagcact tcgcccggcg
     3541 tccccgtcgg gctaaccagc gcgttgacat gggtctgctc accgcccgta gccgcaagcg
     3601 cgcctgccgc gaaaccacaa ccggtaaagg tctggttaac gtgtgctgga tgaatctgcg
     3661 ccgcaatcat cgcagctttg taatactgcg ccgggtcacc cgcccccaga cctactgaaa
     3721 tggacggtat ttccgccatc caacgtttaa cttcctggat cccctcatcc accgaggaga
     3781 actgcgcgga aagcacgcca atcactgcgt ggccttcggc ggcgtcaaag atttcacggg
     3841 cattggcgat atctttcgcc agcacattaa ttgccacgcg attacgatag aaattaatct
     3901 tttgcatgat ctgcaatctc cttcagtcgc gccacgataa gtgccatttc cccttccgcc
     3961 accgtacgcg gatcaaggct aaaaacgccc tggtggaggt tataccgccg tgcataaatc
     4021 gcgatatcgc caccacgcag ttgcgcttcg acgacgcgag cgtcaactcc caattcctgc
     4081 gcatttactc gaatacgaat acgccagatt gcacgcccgg cttcatcctg ctcaatatca
     4141 gccgttaacc cgtgaatcgc agaaatcgct tcaaccactg gctggagctg ttccgcggtg
     4201 atgactgcct gcccctggtg ataattctcc agcgcgtaaa ccagcccaac catgttctcc
     4261 ttgccgattt tcatcgccct ggcaatgccg tgatgctgcg ctttgcaggc tgcaatccag
     4321 atttttttac cagtgataaa cccggaggtc ggcgcgttaa aggcttttgc gccgctgtag
     4381 accaccatat ccgctccgct caccacccag gcacgtaaat cctcttcggc ggcggcatcg
     4441 acaatcagcg gcaggttgta catctgcgcg acatgtgcga agtcctcgat gttcaacatt
     4501 cccttctgca cgcagtgatg cgacttcaca tacagcagcg cggcggtcgc gtcggtaatg
     4561 gcactctcca gctgccagag agccgccagg ttacttgaac ccacctccac aacgcgcccg
     4621 ccacctaagc ggatcgcgct gaggatcgat gcaccgtaat cgacgttatg gccgcgcagc
     4681 atcaccactt cattggccat gccggagcta tccggcatga gcgccacccg ggccttgtcg
     4741 ccgcgtgtaa tggcggcggc gacagcgatg gcgatccctg ctgaggcaca ggaggtaacg
     4801 tagctatcct ccgccccggt aaagcgagag accagctcgc cggttttgtc caccagctgg
     4861 tcaatctcca cgaaagagga tgccgcgcgc gcggtggcct gcatcacctc cggcgcgacg
     4921 ctggagacgc cgagaatcgt catcttgccg caggcgttaa tcacctgttt cagtcccagt
     4981 tgttgataga tattctgcgt catggcttac agcactccca gcagcgagca gaccacgctc
     5041 aacgccacaa tcgacagcag aatcgtggtg taacgcggac cctttttcac caaatagaag
     5101 tagatggcga acactgccgc cagcggcagc aggccgggcg cgatggagtc cagaatctgc
     5161 tgtaccacca cttcagagcc tttcagtgcg ctaattttta aaggtgaggt tatcttgaca
     5221 aagctcgcgg atagcgcgcc catcatgata agccccagta cgttagcgcc gtagataagc
     5281 tccttgatgc gccctccctg cagcagactg ataatcgaat cgcgccccag cgtatagccc
     5341 ttgtgcacca tgccgtagct gattgccagc gtaatcgccg ggtagagaat cagcggcaaa
     5401 ataccgccca tcgcgctgcc attggcggcg aacgggataa aaatagcaat caacagcggc
     5461 atcaccgccg cccagatgat ggagtcgccc atcccggcca gcggccccat caggccggtt
     5521 ttaatcccgg tgatagaggc atcgttaatc gcctcgccgc gggtcttttg ctcttccatg
     5581 gcgatggaga tcccctggat caccgcgcca aacgtctgct ctgagttgaa gaagttcaga
     5641 tggcgtttta acgcctccac ctgctcctcc ttttgcggat agagcttttt gataatcggc
     5701 gtcatcgagg cgcagaaaat caggctctgt aaacgctcat aggagctgga aacttccgcg
     5761 cccagccagt agataaacca cgctttggtg atatccgctt tggtcagcgc cccggactcc
     5821 cgcgcgcgct caaccagttc atgctgcatt acgtcgtcgc tcattatgcc gctccttcat
     5881 ttttcgccag gcctttaatc aggaaggcca cgcaggtgcc gaaaatcgcc atcgccatga
     5941 tgtccacctt gaggtacagc accgcgaaga atccgccgat aaaccacggc agcaggctct
     6001 ttttaccaat caccataatg gtgatggcga agcccagcgc cgggagaatc ccgcccatga
     6061 tttcaaacga gtgggtcagc cagtgcggca tcagcttgag gaaactttcc actacgttct
     6121 ggccgaagta gttggcggcg aacaccactg ggaaacgtag taccagcccc agcagcgccg
     6181 ggtagagaaa ggcgcagcgc ataatggctg acatattggc cgtttccgcg tgtttatcgg
     6241 ccatatgcac ccaggccgca tttagcgtgc gacgcaactg gtcgaggaat acgccgataa
     6301 cgccaaatgg aatcgccagt gcaatcgcca ggttcgggtc catattggcc ttaacggcga
     6361 taggaatggc gatacaggcc gccagcgccg ggtctgacgg cacattgccg cctggcgttg
     6421 aggtcacacc cagatagacc aactgcatcc ccgcgccgat aatcatcgcc gcaggcatat
     6481 tgccgagcag caggccgaca aagaccgcca ccaccactgg ctgtaacagc atggcggaga
     6541 aggtatagcc aaggcgcaac cgggcaaacc agtaatacag ccccatcagg cttgcaaaaa
     6601 gtaaggtatc cataatttgt tatcccatgt tcagagggtc agaatttttt caggatatcg
     6661 tccagcgact gcggtttatc ttccgggatg gtctggaaaa cagccttaat gccgcgactt
     6721 ttcaaatcgt taagaatgcc aacgtctttt tcatccagcg ttatgttctg gaacaccgct
     6781 ttacggtttg gcccgccgcc taaaccgcca acctgaatat cagccacgtc aaagccctgc
     6841 tgcaccgctt cctgaatcgc cgccagcgac gggaacagca ccagcacgtt gccgtcgccc
     6901 agctggttct ctttccacgc ggccgcgaag ctctggttgc taaagcagtc cactttgata
     6961 ttcggcggcg cggccatcag gtagatattt ttcataaacg gatcggcgtc cagctcgtcg
     7021 ctgaccaccg caatacggtt ggcctgagac tgccctaccc acttcgtcac cacctgtccg
     7081 tggataagac ggctgtcaat gcgacataac acgatgtttg ccatacgatt ttccttactg
     7141 ttgtaattga cgaatatggg agacgacgtc caggcaactg ttgcgcccag cttccaccag
     7201 atcggccacg caggcggtca gtgggccatg ttcgcgcttg tccagcgcct ccagcagcag
     7261 cgaggcatta agcccggaga tcaccgccac cggataatcg gcgctcagcc gcgccgccac
     7321 gttagaggtg gtgccgccga taaaatcggt cacgataagc gaaccttccg gcatggtttt
     7381 caccacggct tcgacgcgct ggtaaaactc gcccagcgta tcgacgggca tcagcgcgat
     7441 ttccgtcacg ccttcgattt cgcccatcac catgcgcaga ctgttgcaga gctgcagccc
     7501 ccagccgccg tgagttagca gcagaatttc aggtaacgga tgaataggta cagttgtggt
     7561 caaaatagcc tcctttgctc cagtgtttac atttcatcag gagcaaagga cgtgccagaa
     7621 tttgtgtcac gcacagagga aatgagattg gatggggcgt taagccccgt aaaccagggg
     7681 atcagctgta gagcagttcg tagatataaa aatattctgc gtccgataaa cggatagcgt
     7741 aagcctcttc aatcggcaaa aaggaggatt tgatgacgct aaaggcacga gcgtccagat
     7801 caagccggtt ttctagcgcc atctgtaacg gtttgcggtt aatcacgata cgctcgacca
     7861 tgcagcagca gtggatcaga aaacgtagtg tcacctggcg gctcggtttc agggagagcg
     7921 tgttggttaa gtagttcaga acatcctcga tctctttcag aattcgctgc gggttgagca
     7981 ccgaaatatg gttgatgatg ctctccatgg tcagcgcgct gataaagcgc atggcgctgc
     8041 gctccatctc cagtcggcgc tcgctgcttg agagatccgg cgtcagcagg ctgagcacca
     8101 gctccggccc ttgctcggaa aacagttcct ccagcgagat aaacggtata tccggcagtc
     8161 ccggctggaa ggtgccgacg atccctgcca gccgttcact ggcattaagc gcctgctgaa
     8221 tacgctccag gctgcgcacc tcgttatagt cgagaatcac catacgcgtg tcctgcgaca
     8281 tcagctcgcc aaagctctcc tccagcacct ttttgatctt ttccgccgtc cccattccgg
     8341 ttatacatga gataaccagc actttgccac cgctctcttg ctgcggcgtg cagagccgac
     8401 aagggatatt tttactctgc ataagcacag ccaattgcga tagatcgcca gtttcgtagc
     8461 ttaaatccag cccaacctcc agcaggctgg tcagagtgat atttggcaac aacagaacgt
     8521 caatctggaa cagcttgctg atggtgctgc caaaatggac cagcgatccg atatccacca
     8581 tcagtatcag ccgctgccag caccggtcct gaatcatccg ggtcagggtt tccagggtgt
     8641 catgcaccga ctgctcaaat ggcatatcaa ttgcgtagaa cagatcgcgc tccagcaccc
     8701 ggttcacata ttgcgcctgg ctcgttgcgg tggtcgcacc gtgagcgatt agaatcacgc
     8761 cgcaatccgg gctggtgtcg atgcgctggc ggtagtggcg acactctttc aggaacaggc
     8821 acagccagac cacctccgtc gccgggcact gaatatgcaa caactcattg attttgcggc
     8881 acagaagcgt ggcattgtca tactcatctt tacagcgatc cagaatcagg ctggatgaat
     8941 agagttgagg tataagccct cgccgcacat agccaatcag cgcaagaaag tgtttacgta
     9001 acgggttcgc caggttttct ggcaatgtaa atcctaatac ctgctccacg taaccgatca
     9061 gcagcgtcac acgctcctca atttgatcgc catagcgcgg tggatgcgcc gcactatggc
     9121 ggctataaag accatattca aaaatcgaac tgagtttgtt tttcaggata gccagcgttt
     9181 ctgcgggcgg cacgttactg ttgcgcagat tgacatattc acgagtcagg aaactgtaaa
     9241 acaggtcgct ctcttctatt tccgcgctgg tcgccaacga attttttaac gcgggcaacg
     9301 tacgcgcatc cacgttcaga cgatctttac cgccaaacaa cgcatcgacc agcagacgct
     9361 gctctggcgt ggtattaaac gacatctccg ccagtcgttt atctaactct agcgtatcat
     9421 tacgctcggt cattcccgac gcccacgcct gcgcgcacaa gaactgaata tcacttttaa
     9481 gctggccgat attgccttcc aggggtttac ttaacaacca atgcaagagc gttttatcaa
     9541 ggcttatggt acgttcaatt ttacgacttt cacgctgaaa gaagcttaca atcaattcga
     9601 gctgctcctc gaccgaacgc tggcgaatac ccggtaagtc aatacatacc tggatacgcc
     9661 gctggaaggt acgcaatagt gccgagttga ccgactctgt agtggcgcaa ataaggcgca
     9721 ccgaaattga atatgttggc ccacttgaac caagtgggcg gtattcacct ttatcgagaa
     9781 tagaaaagag cttctcttgt ccttcgtacg ggaggcgatg cacttcatcc agaagtaaat
     9841 aacctccgtc agcttgctcc accaatcccg ttttattctc acttgccccg gtaaatgccc
     9901 ccttatggtg accaaacaga tgtgaggaaa gcaactccgg attatgagca tattcggcac
     9961 agttaaagta aaccaacgga ggtggcgaac ctatggtgtg cttgcaagcg aagcaatgca
    10021 ttaactcagc aaagaatgtt ttgccaacac cagatgcacc ggttaacagc acatgtagtc
    10081 cgtggggata aagcaccgct gcgcggcctt tttctaccgc atcacggaga ctacggtcgt
    10141 atccgataag tccggaaaac ggatcatcat ctgtatattt ttctttcttt ggcaataaac
    10201 tagccactga atgaactatg cattcgcaat catccagttt tcgattaatg agcaattcta
    10261 gtgcgtgacg atgtagaaaa aaaacaggac gtccctgact ttttaccacc aggccttcat
    10321 tccataattg attaagatct ttactaaccg agttgcgtgc cagcccaagg ttaaaaccaa
    10381 tcgcctcagc agtaaatgct ttctccttcg ctaaatcaga cagttccagc ccatacgtca
    10441 gccgctccag ctccttgtga ataacatcaa tccgtctcat ttctttaccg cttaaaaaac
    10501 aatagttgtt cagaacatac actatttgta aacgtaagaa tgagaactat cgggaagaat
    10561 taatgttcct tgtggcaatc tggtttctga atattcgtgt tttaaatatt acttatggtc
    10621 tgaaacaacc aggcaggtga acagagctaa gaaaaagtag cgtagaaata taaaaacata
    10681 tatcgcactg atgaatgttt cccggcatga ctatgcacac cgggaaatat ttagtaaatt
    10741 actcgccctt tttcgccgcc tgaatataca gcatctccag cgccagcgtc gccgcagcca
    10801 gagcagtgat ttccgactga tcgtatgccg gagccacttc cactacgtcc atcccaacga
    10861 tgttgagatc tttcaggccg cgtaccagtt taatggcgcg atcggaggtc agaccgccaa
    10921 ttaccggcgt accggttccc ggcgcaaatg caggatccag gcagtcgata tcaaaggtca
    10981 ggtaaaccgg catatcaccc acaatttgtt tcacctgggc gataacgtca tccacgctgc
    11041 gatcgttcac ctggcaggcg tccagcacgg taaagccgtt gtctttatca aattcggtac
    11101 gaataccaat ctgcacggaa tgattcggat cgatcagacc ttctttcggc gcggtataga
    11161 acatggtgcc gtggtcaaat tcacaaccgt tcgcgtaggt atcggtgtgg gcgtcaaagt
    11221 gtaccagcgc cattttgccg aaatgcttcg catgagcacg cagcagcggc agcgtaacaa
    11281 agtggtcacc accgaaggag agcatacgct taccggcagc cagcagcttc tcggcgtgcg
    11341 cctgcagctt ctcgctcatc tcacgggcat cgccgaaagc atataccaga tcaccgcagt
    11401 ccacgacgtt cagacgctca cgcatgtcga aattccacgg gaagcggttg tgttcccagg
    11461 ccagattcgt cgaaacctga cggatagctg ccggaccgtg gcgtcccccc gcacgaccag
    11521 aagtggccat atcgaacggc acaccagtaa tcacccagtc agcatcgctg tcatacggct
    11581 ggaagttcat cggcaggcgt aaaaaaccaa aggcattgga aaccagtgag ttatcgtatt
    11641 gatgacctaa ggtgctcatg gatggactcc tattacaaag tcgatataaa aaaacccttc
    11701 cgcgtcgtta ggcccgacga ggaagggttg gatttgcgaa acacaagttt gtgctaatta
    11761 tcgccttaaa tgggcgaggc atcaagcgag gttgctattt ccgacacggt ggcggcgcat
    11821 agccattgtt atctgccgga aaggcttgtc cagagcacgg cggcagcgcg ttttgcgcct
    11881 ggccttgtaa ttcaaaggcg gcaaagccta ttacaccggt attacgcaca tcgccttgcg
    11941 ccgaattggc tgcgtaggaa tcagcaacgt tggcgaactg aaaagccgct tcggtatgtt
    12001 tatctttgcg gaatccttta atcgctaaag aatctccggc attcacaata tagccgttgt
    12061 tattcagcga accttgcttg ccgttcagca catccaggcc atcaacagta gcaacgatct
    12121 cgtagttggt gttacgactg taattacgga catataattg ataattcatt ccgactctgg
    12181 catggacaat atagcgtggt tcattattgg atgaatcata aacacgcgta ataggtagag
    12241 aattaaaatt accatcgcga atagcatatt ccagttcgtt aatacgaatg ctataaacgc
    12301 ggtcatagcc ggaaggaaca ttagtcgaat aactaataac gatcatctct gccggatcgc
    12361 gccaggctcg ctcagcagat acgccctgaa ccgatgagtg cacatcccca ccccaggcag
    12421 tgccgacatt gcccgatgca ctgctattat actgcgcatt actggaacaa ccggccagca
    12481 gcacgattaa tgccaccaac gcgctacgta ctttccattt tttcataacc gttccctctg
    12541 catttaatta atgcagcgat tgtagggaga ttatttaata agaaaagcag cgagattatt
    12601 tttaagaata tataaaaaga aagcgggccg atttacgtaa ataaatcgac ccgccagcgg
    12661 aataatctta attattcatc ttcaagatag gtataaccgt acaaacctgc ctcgaactct
    12721 tcaaggaact gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac
    12781 tgggttaaca gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg
    12841 ccttcgtcag acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaaccgct
    12901 tcggtatcac cgaacaggtt gtgcatattg ccaaggatct cctgatatgc gccgaccata
    12961 aagaaaccga gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca
    13021 ataccatcac catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc
    13081 agcactgcgc gacgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac
    13141 tggtcgatcc cccatgcatc cggcattgac tggaacagcg agaaattgac gtacattttg
    13201 tccgccatac gttcctgcag ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc
    13261 agctgctttt gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct
    13321 tgcaggctaa aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctggctg
    13381 tcgtgtaacc attcacgcag agaacggcga gttcccggtt cgtgcatttc ctgccaggtt
    13441 tcccacatgc tttgcagcgc gcgcggcgca tcttctgcag gcgcggtcgg caccgtgtat
    13501 tcgttacgtt ccacgccgat gatattagat accagcacgg tgtgatgcgc agtcaccgca
    13561 cgacccgatt cggtgattac cgtcggatgc ggcagaccgt tttcttcaca cgcatcacca
    13621 atcgcccaga tgatgttgtt ggcatattca ttgaggccgt agttcaccga acagtcggac
    13681 tgcgaacggg tgccttcata gtccacgccc agaccgccgc cgacgtcgaa gcactgaata
    13741 ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcctgtcgcg
    13801 atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt gcagcagttg caggctgtcg
    13861 agacgcccgg cttcacgcag ggtttcaaca agttgcagta cctgagtcgc agccaggccg
    13921 aacttcgatt tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt
    13981 gcacgcacgc ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg
    14041 atttctgaca tcttctcaat gaccaggtag accttgtggc ccatcttctc gccaattaat
    14101 gccaggcgga tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg
    14161 ccagcatgtg ccagcaccgc catcaactct gctttcgagc ctgcttccag tcccagcggt
    14221 tcgcccgaat gaatcaggga ctcaatcacg cggcggtgct ggttaacttt gatcggataa
    14281 acaaggaagt aatcgccgtt atagccgtag gattcccgcg cacgtttgaa cgcggcgtta
    14341 atggaacgca aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga
    14401 ccctgtgctt cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc
    14461 gggtccgggc acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac
    14521 caggcaatat tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc
    14581 tgcatggagc gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg
    14641 tcagacatag cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag
    14701 cctggaactg cgataacaac ccataagcac atggattttc cagcagtgta tgctgacgct
    14761 ccgactgcgc aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg
    14821 tgacagttcg gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc
    14881 ctcagtaact gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc
    14941 aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca
    15001 ggttaagcag cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac
    15061 gaacccacaa gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg
    15121 tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg
    15181 caaaagataa atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa
    15241 cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc
    15301 catccagatg ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacttg
    15361 cagagtcgtg gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa
    15421 ttctctttag gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga
    15481 agggcatcct gacaaaattg ctgaccaaat ttctgatgcc gttttagacg cgatcctcga
    15541 acaggatccg aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt
    15601 tggcggcgaa atcaccacta gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt
    15661 tcgcgaaatt ggctatgtac attccgacat gggctttgac gctaactcct gtgcggttct
    15721 gagcgctatc ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct
    15781 ggaacagggc gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt
    15841 gctgatgcca gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg
    15901 taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta
    15961 tgacgacggc aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga
    16021 gatcgaccag aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc
    16081 cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat
    16141 cggtggcccg atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg
    16201 cggcatggca cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg
    16261 ttccgctgcg tacgcagcac gttatgttgc aaaaaacatc gttgctgctg gcctggccga
    16321 tcgttgtgaa attcaggttt cctacgcaat cggcgtggct gaaccgacct ccatcatggt
    16381 agaaactttc ggtaccgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt
    16441 cttcgacctg cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa
    16501 agaaaccgca gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa
    16561 agcgcagctg ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag
    16621 gccagcctcg cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc
    16681 atccggcaaa aagctgcccg cacaaaaaca tcattcttcc tcaacacgtt ttatcgcaaa
    16741 ttatcatcat tactgaaacc gattacacca ataacaccag ccaaatattt gtaata
//
LOCUS       DAZFRJ010000095        16621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000095 DAZFRJ010000000
VERSION     DAZFRJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.095"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..461
                     /locus_tag="ACHKUN_002820"
     CDS             <1..461
                     /locus_tag="ACHKUN_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6661046.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRG
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            830..3676
                     /locus_tag="ACHKUN_002821"
     CDS             830..3676
                     /locus_tag="ACHKUN_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO6661047.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGGYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            4004..4822
                     /locus_tag="ACHKUN_002822"
     CDS             4004..4822
                     /locus_tag="ACHKUN_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6661048.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4914..5399
                     /locus_tag="ACHKUN_002823"
     CDS             4914..5399
                     /locus_tag="ACHKUN_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6661049.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            5415..5891
                     /locus_tag="ACHKUN_002824"
     CDS             5415..5891
                     /locus_tag="ACHKUN_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6661050.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            5954..6175
                     /locus_tag="ACHKUN_002825"
     CDS             5954..6175
                     /locus_tag="ACHKUN_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6661051.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6249..6656
                     /locus_tag="ACHKUN_002826"
     CDS             6249..6656
                     /locus_tag="ACHKUN_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6661052.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(7197..8444)
                     /locus_tag="ACHKUN_002827"
     CDS             complement(7197..8444)
                     /locus_tag="ACHKUN_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO6661053.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8516..9430)
                     /locus_tag="ACHKUN_002828"
     CDS             complement(8516..9430)
                     /locus_tag="ACHKUN_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6661054.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9646..11079
                     /gene="cscA"
                     /locus_tag="ACHKUN_002829"
     CDS             9646..11079
                     /gene="cscA"
                     /locus_tag="ACHKUN_002829"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6661055.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(11087..12082)
                     /locus_tag="ACHKUN_002830"
     CDS             complement(11087..12082)
                     /locus_tag="ACHKUN_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6661056.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            12325..12543
                     /locus_tag="ACHKUN_002831"
                     /pseudo
     CDS             12325..12543
                     /locus_tag="ACHKUN_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHKUN_002832"
     CDS             12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHKUN_002832"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO6661057.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHKUN_002833"
     CDS             complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHKUN_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO6661058.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15535..>16621)
                     /gene="emrK"
                     /locus_tag="ACHKUN_002834"
     CDS             complement(15535..>16621)
                     /gene="emrK"
                     /locus_tag="ACHKUN_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO6661059.1"
                     /translation="AFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNH
                     KDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQ
                     YQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPL
                     NRQPQVVEAADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQ
                     MWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNAT
                     GNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPA
                     YTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         4346 a         4243 c         4111 g         3921 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg ggccgtccgt gactcggtgg
      421 tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgccccc ccctcatacc cacctctttc
      541 tcttgcgtga cttccctgct gttaatagaa taaaacgatc gataaaacag gtatcgatcg
      601 ttactatcga tcgtttatag tggttaatgg tctgcgaaaa ggcagctgaa tctcttcatc
      661 atgcagaacg gaattgcaca caacggactg atacttctct ggcggatgag agaggggaaa
      721 catttatgtc cgggaaaaac cactttatct gaccatgctg ttttgtacct gccggtatcc
      781 acttttgtgg gtaccggctt ttttattcac cctctgtaag gaaaagctga tgaaacgaca
      841 tctgaacacc agctacaggc tggtatggaa tcacattacg ggcaccctgg tggtggcctc
      901 cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg gttgcgctgt ctcttgctgc
      961 tgtcacatca gtcccggcac tggctgctga cacggttgta caggcgggag aaaccgtgaa
     1021 cggcggaaca ctgacaaatc atgacaacca gattgtcttc ggtacggcca acggaatgac
     1081 catcagtacc ggtctggagt atgggccgga taacgaggcc aataccggcg gacaatggat
     1141 acaaaatggc ggtatcgcca acaacactac tgtcaccggt ggtggtcttc agagagtgaa
     1201 tgccggagga agcgtttcag acacggttat cagtgccgga ggcggacaga gccttcaggg
     1261 gcaggcagtg aacaccactc tgaacggcgg tgagcagtgg gtacatgaag gcgggattgc
     1321 aacggttacc gtcattaatg agaagggctg gcaggccgtc aaatccggcg caatggcaac
     1381 cgacacggtt gtgaataccg gcgcggaagg gggaccggat gcagaaaatg gtgataccgg
     1441 gcagaacgtc tacggagatg ccgtacgcac caccatcaat aaaaatggtc gtcagattgt
     1501 ggctgctgaa ggaacggcaa ataccactgt ggtttatgcc ggcggcgacc agactgtaca
     1561 tggtcacgca ctggatacca cgctgaatgg ggggtaccag tatgtgcaca acggcggtac
     1621 ggcgtctggc actgttgtaa acagtgacgg ctggcagatt atcaaggaag gtggtctggc
     1681 ggatttcacc accgttaacc agaaaggcaa actgcaggtg aacgccggtg gtaccgccac
     1741 acatgtcacc ctgaaacagg gtggagcact ggtcaccagt actgcggcaa cggttcttgg
     1801 cagcaaccgt ctgggcaatt tcacggtgga aaacggtaag gctgacggcg ttgttctgga
     1861 gtccggcggt cgcctggatg tactggaagg ccattcagcg cagaaaacac gggtggatga
     1921 cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca gatgtcacca tgacatccgg
     1981 tggtgccctg atagcagaca gtggtgccac tgttgagggg accaatgcca gcggtaagtt
     2041 cagtattgat ggcatatccg gtcaggccag cggcctgcta ctggaaaatg gcggcagctt
     2101 tacggttaat gccgggggac aggcaggcaa caccactgtc ggacatcgtg gaacactgac
     2161 gctggctgcc gggggaagtc tgagtggcag aacacagctc agtaaaggcg ccagtatggt
     2221 actgaatggt gatgtggtca gtaccggcga tattgttaac gcaggagaga ttcactttga
     2281 taatcagacg acacaggatg ccgtgctgag ccgtgctgtt gcaaaaggcg actccccggt
     2341 aacgttccat aaactgacca ccaccaacct cactggtcag ggcggcacca tcaatatgcg
     2401 tgttcgcctt gatggcagta atacctcaga ccagctggtg attaatggtg gtcaggcaac
     2461 cggcaaaacc tggcttgcgt tcacaaatgt cggaaacagt aacctcgggg tggcaaccac
     2521 cggacagggt atccgggttg tggatgcaca gaatggtgcc accacagaag aaggtgcgtt
     2581 tgccctgagc cgcccgcttc aggccggcgc ctttaactac accctgaacc gtgacagcga
     2641 tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct gaagtccccc tgtatgcctc
     2701 catgctgaca caggcaatgg actatgaccg gattctggct ggctcccgca gccatcagac
     2761 cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt cagggcggtc atctcgggca
     2821 cgataacaac ggcggtattg cccgtggggc cacgccggaa agcagcggca gctatggctt
     2881 cgtccgtctg gagggtgacc tgctcagaac agaggttgcc ggtatgtctc tgacgacagg
     2941 ggggtatggt gctgcaggcc attcttccgt tgatgttaag gatgatgacg gctcccgcgc
     3001 cggcacggtc cgggatgatg ccggcagtct gggcggatac ctgaatctgg tacacacatc
     3061 ctccggcctg tgggctgaca ttgtggcaca gggaacccgc cacagcatga aagcatcatc
     3121 ggacaataac gacttccgcg cccggggctg gggctggctg ggctcactgg aaaccggtct
     3181 gcccttcagt atcactgaca atctgatgct ggagccacaa ctgcagtaca cctggcaggg
     3241 actctccctg gatgacggcc aggataacgc cggttatgtg aagttcgggc atggcagtgc
     3301 acaacatgtg cgtgccggct tccgtctggg cagccacaac gatatgaact ttggtaaagg
     3361 cacctcatcc cgtgacaccc tgcgcgacag tgccaaacac agtgtgcgtg aattgccggt
     3421 gaactggtgg gtacagcctt ctgttatccg caccttcagc tcccggggag acatgagcat
     3481 gggtacagcc gcagccggca gtaacatgac gttctcaccg tcccggaatg gcacgtcact
     3541 ggacctgcag gccggactgg aagcccgtgt ccgggaaaat atcaccctgg gcgttcaggc
     3601 cggttatgcc cacagtgtca gcggcagcag cgctgaaggt tataacggtc aggccacact
     3661 gaacgtgact ttctgataat caggtcactt cccttcaggc cccggtcatt atgaccgggg
     3721 ccttttactt tcagggatca acagatatgc cgggttgtgc atcacgttta ttgcccgaag
     3781 acccttttac tgccaaatgg catctgaatt gatcacagtc tggccagtta ttacccgatc
     3841 ccgatgggga tattgggtga tctccttcca tgaaaatacg cagctcacca gtggagggct
     3901 tctcagacaa taaacgtcat tcatttcatc tgtcaggccg cgtcttctcc gggagacgcg
     3961 gctttttcat ttataccgct aactcattca taaggagcaa ataatgcgat tagcaagtcg
     4021 ttttggtcat gtaaaccaga tacgccgtga ccgcccgctg acacatgaag aactgatgca
     4081 ctatgtgccc agtatttttg gggaagaccg gcatacctcc cgcagtgaac gctatgcgta
     4141 cattcccacc atcaccgtac tagaaagcct gcagcgggag ggctttcagc ccttcttcgc
     4201 ctaccagatc cgcgtgcgcg acccgggccg ccggggatac accaaacaca tgctgcgtct
     4261 gcgccgtgac ggggagataa acggacaaca tgtccctgaa atcatcctgc tgaactccca
     4321 tgacggtact tccagctacc agatgctgcc gggttacttc aggttcgtct gccagaacgg
     4381 gtgcgtctgt ggccagtctc tgggggaagt gcgtgttcca caccggggaa atgtggtgga
     4441 gaaagttatc gaaggggctt acgaggtggt gggcgtgttt gaccggatag aggagaagcg
     4501 tgatgccatg cagtcgctgg tcctgccgcc accgtcacgc caggcgctgg cacaggcggc
     4561 actgacttac cgttatggtg acgaacatca gcccgtcacc accgccgaca ttctgacgcc
     4621 acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca tccaggagaa
     4681 tatgctgaaa ggcgggattt ccggtcgcag tgccagagga aaacgtatcc atacccgtac
     4741 cattcacagc atcgacaccg acattaagct caaccgggcg ttgtgggtga tggcagaaac
     4801 actgctggag agcatgcgct gatgcctttt ccctgaaagc gcagtcctga ctcacttccg
     4861 tccctgcctc cggacattct tccattcatt tactttttat aaggaataat ctcatgacaa
     4921 ccgttacgca taattccacc acaccttctg tttccgtaac cgctgcatca gggaataacc
     4981 cacctcagct ggttgctact ctcgtccctg atgaacagcg catcagcttc tggccgcagc
     5041 attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
     5101 gtctgtgcga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     5161 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     5221 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg tattgccgcc tgtctgatga
     5281 cgtacagtca tcatgcctgt cgtatggaga attatgccat gacggtccat tattaccggt
     5341 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     5401 tggagagaag cacaatgcaa caactttcct ttctgcccgg agagatgacg cccggcgagc
     5461 gcagcctcat tcaacgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     5521 ccttcacctc cacccgtgca gcacgggaat ggctgattct gaacatggcg ggactggagc
     5581 gtgaagaatt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     5641 tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt aaacgcgccc
     5701 tgtaccacaa tgccgctgcc gtggtactgg cacacaatca cccgtccggt gaagtcacac
     5761 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     5821 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     5881 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     5941 gaggtatccc attatgaaaa ttatcacccg tggtgaagcc atgcgtattc accaacaaca
     6001 tccgacatcc cgtctttttc cgttctgtac cggtaaatac cgctggcacg gcagtgctga
     6061 agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg
     6121 ccgtaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgagtggg
     6181 aattctgatg agcagaatta tcgccactac cggaccattc ttagccgatt ttctgtaagg
     6241 attttatcgt gtcagacaca ctccacggga caacacttcc cgacgacaat cacgaccgcc
     6301 cctggtgggg gctgccctgc accgtgacgc cctgtttcgg ggcacgtctg gtgcaggagg
     6361 gtaaccggtt gcattacctt gccgaccgcg ccggtatcag aggccggttc agcgatgcgg
     6421 atgcatacca tctggaccag gcctttccac tgctgatgaa acaactggaa ctcatgctca
     6481 ccagcggtga actgaatccc cgccatcagc ataccatcac gctgtatgca aaagggctga
     6541 cctgtgaagc tgatactctc ggcagttgta gtttcgttta tatggctgtt tatgctaccc
     6601 gattctcaaa tcaattaccg aaagcttctg ttcatcaact aactcattac ttttaatgat
     6661 ggggcccttt ctggggccca agaaattaac aacgctaaca aacaacataa ataacaataa
     6721 attaaataaa aaatttcatt cctgcagggg acaccagata cccttcaaac gaaatctacc
     6781 ttcaccccgt aaaagatagg tttggcagca cacttgcctt atatctactc atttttactg
     6841 caacaggttg aaatctcagc actgtcagaa agcgctgatg actaaacagc cctgggccgg
     6901 gcgatgtaac catcacacag aatcctgata gcgaaatatg gcgtgactcg atacttcact
     6961 ccgcaatgca ttccttgatg aattcgcagg accgtgatac acgggacagg tcgctgaatg
     7021 acgacaatgt cctggaaatc agcgaaccgc gcatctgaag tacatttgag cgactgtacc
     7081 agaacatgaa tgaggcgttt ggattaggtg attattagca gggctaagca ctttagtatt
     7141 attattttcc ggttgaggga tagggagata tcgacaacaa ccggaaaagt ttacgtctat
     7201 attgctgaag gtacaggcgt ttccataact atttgctcgc gttttttact caggaagaaa
     7261 atgccaaata gcaacatcag gcagacaata cccgaaattg cgaagaaaac tgtctggtag
     7321 cctgcgtggt caaagagtat cccagtcggc gttgaaagca gcacaatccc aagcgaactg
     7381 gcaatttgaa aaccaatcag aaagatcgtc gacgacaggc gcttatcaaa atttgccacg
     7441 ctgtatttga agacggatat gacacaaagt ggaacctcaa tagcatgtaa cagcttcact
     7501 aatgaaataa tccaggggtt aacgaacagc gcgcaggaaa ggatacgcaa cgccataatc
     7561 acaacaccga taagtaatgc attttttggc cctacccgat tcacaaagaa aggaataatc
     7621 gccatgcata gcgcttcgag taccacctgg aatgagttga gataaccata caggcgcgtt
     7681 cctacatcgt gtgattcgaa taaacctgca taaaagacag gaaaaagttg ttgatcaaaa
     7741 atgttataga aagaccacgt ccccacaata aatatgacga aaacccagaa gtttcgatcc
     7801 ttgaaaactg cgataaaatc ctcttttttt acccctcccg catccgccgc tacgcactgg
     7861 tgatccttat ctttaaaaca catgttgatc atcataaata cagcgccaaa tagcgagacc
     7921 aaccagaagt tgatatgggg actgatacta aaaaatatgc cggcaaagaa cgcgccaata
     7981 gcatagccaa aagatcccca ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc
     8041 attttttcgg tgaagctgtc aagcaaaccg catcccgcca gataccccag gccaaaaaag
     8101 agcgccccca gaattagacc tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa
     8161 atcataaacg gtccggtcaa gaccaggatg aaactcatac accagatgag cggtttcttc
     8221 agaccgagtt tatcctgaac gatgccgtag aacatcataa atagaatgct ggtaaactgg
     8281 ttgaccgaat aaagtgtacc taattccgtc cctgtcaacc ctagatgtcc tttcagccaa
     8341 atagcgtata acgaccacca cagcgaccag gaaataaaaa agagaaatga gtaactggat
     8401 gcaaaacgat agtacgcatt tctgaatgga atattcagtg ccataattac ctacctgtcg
     8461 ttaaaaaatt cacgtcctat ttagagataa gagcgacttc gccgtttact tctcactatt
     8521 ccagttcttg tcgacatggc agcgctgtca ttgccccttt cgccgttact gcaagcgctc
     8581 cgcaacgttg agcgagatcg ataattcgtc gcatttctct ctcatctgta gataatcccg
     8641 tagaggacag acctgtgagt aacccggcaa cgaacgcatc tcccgccccc gtgctatcga
     8701 cacaattcac agacattcca gcaaaatggt gaacttgtcc tcgataacag accaccaccc
     8761 cttctgcacc tttagtcacc aacagcatgg cgatctcata ctcttttgcc agggcgcata
     8821 tatcctgatc gttctgtgtt tttccactga taagtcgcca ttcttcttcc gagagcttga
     8881 cgacatccgc cagttgtagc gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc
     8941 atagatcttc acgaatatta ggatcgaagc tgacaaaacc tccggcatgc cggatcgccg
     9001 tcatcgcagt aaatgcgctg gtacgcgaag gctcggcaga caacgcaatt gaacagagat
     9061 gtaaccattc gccatgtcgc cagcagggca agtctgtcgt ctctaaaaaa agatcggcac
     9121 tggggcggac cataaacgta aatgaacgtt ccccttgatc gttcagatcg acaagcaccg
     9181 tggatgtccg gtgccattca tcttgcttca gatacgtgat atcgactccc tcagttagca
     9241 gcgttctttg cattaacgcc ccaaaaggat catcaccgac ccgacctata aatccactta
     9301 ttccgcctaa tctggcgatt cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca
     9361 gtaggcgccc gtctgattct ggcaagagat ctacgaccgc atcccctaaa acccatactt
     9421 tggctgacat ttttttccct taaattcatc tgagttacgc atagtgataa acctctttct
     9481 cgcaaaatcg tcatggattt actaaaacat gcatattcga tcacaaaacg tcatagttaa
     9541 cgttaacatt tgtgatattc atcgcattta tgaaagtaag ggactatatt tttataaaag
     9601 ttaacgttaa caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga
     9661 ttgcatgcgg cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat
     9721 ccccattttc acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg
     9781 tttaacgatc gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca
     9841 atgcactggg gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg
     9901 ctagcgccag gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac
     9961 aatggtgtcc tctcacttat ctacaccgga cacgtctggc tcgatggtgc aggtaatgac
    10021 gatgcaattc gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa
    10081 cagggtgtga tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg
    10141 cgtgaagccg acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag
    10201 atcctgcttt atcgcggcag ttcgttgcgt gaatggacct tcgatcgcgt actggcccac
    10261 gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag
    10321 cattatctga tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc
    10381 tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg
    10441 cattttactg aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag
    10501 gatggtcggc gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa
    10561 cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa
    10621 cttctacaac gcccggtaca cgaagctgag tcgttacgcc agcagcatca atctgtctct
    10681 ccccgcacaa tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag
    10741 ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga
    10801 atgcggctgt atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac
    10861 gagaatttag acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg
    10921 atatttatcg atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt
    10981 agtcgaatct atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg
    11041 gctgtgctgc aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac
    11101 ggatcaacag cgggcaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat
    11161 gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta
    11221 aaaacagatg cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga
    11281 ttcgtacccc cttcgccaga agaacctgat aagccacaaa ggctgcgcga tcgttaccac
    11341 atatcagaac atcaaaatct ggtttgcccg atttgaagtg gtcattgagt aaacttgcga
    11401 gatcggtgta gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc
    11461 gtccagcatc acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac
    11521 tttccggtag ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg
    11581 attcatattg tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca
    11641 cacagttcgc caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca
    11701 gccccattgt agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc
    11761 gggcagcgtc atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg
    11821 cggtttgctc aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg
    11881 ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat
    11941 caggaacata attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca
    12001 cagattctgc attatgcatc acccgggaga ctgtcatcat cgacactccc gctaggcgtg
    12061 cgacatcctt taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga
    12121 aacagacgtc actggcgaga tccaacgata ttctcaacgc cctatcaccc gacacggcaa
    12181 tacaataaaa aataacaata attgccggat aattgtactc aattccgcct ctgttctcgc
    12241 caacgagtaa ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag
    12301 aagaaaatat aaatatggat cattgtgctg ccgctgtatc ccgacatcag cccggaaatt
    12361 attgcgattg ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc
    12421 tggctagtga agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca
    12481 gcgacattta tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc
    12541 taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc
    12601 cgttggtaaa ggatctggtt gctcttaaag aaaccacctg gtttaatcct ggcacgacct
    12661 cattggctga aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg
    12721 cgcgcttatc ccgttttgca ccctatctgg caaaagcatt tcctgaaact gctgccactg
    12781 gggggattat tgaatcagaa ctggttgcca ttccagctat gcaaaaacgg ctggaaaaag
    12841 aatatcagca accgatcagc gggcaactgt tactgaaaaa agatagccat ttgcccattt
    12901 ccggctccat aaaagcacgc ggcgggattt atgaagtcct ggcacacgca gaaaaactgg
    12961 ctctggaagc ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt
    13021 ttaaacagtt ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat
    13081 caatcggcat tatgagcgcc cgcattggct ttaagatgac agttcatatg tctgctgatg
    13141 cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc
    13201 aagattatgg tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt
    13261 tctttattga tgacgaaaat tcccgcacgt tgttccttgg gtattccgtc gctggccagc
    13321 gtcttaaagc gcaatttgcc cagcaaggtc gtatcgtcga tgctgataac cctctgtttg
    13381 tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcgga cttaaactgg
    13441 cgtttggcga tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt
    13501 taggcgtcca tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc
    13561 ttaccgcagc ggatggcctt gcagttggtc gcgcatcagg ctttgtcggg cgggcaatgg
    13621 agcgtctgct ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct
    13681 ggctggcgca ggaagaaggt attcgtcttg aaccttcagc actggcgggt atggccggac
    13741 ctcagcgcgt gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaat
    13801 tgcgtaatgc cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga
    13861 tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa cgcagcatcg caatcctttc
    13921 cctgggtgag cgatgctgcc gatggcgcag acttaagatc cccgctctta cccgctataa
    13981 ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
    14041 ccaaaaccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
    14101 tagaaagtga ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac
    14161 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg
    14221 ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg
    14281 acgtaccaac tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa
    14341 atttattatc tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa
    14401 cggcgaatcc ctggaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
    14461 atgtcacaga acgccagtaa tagcaaaccg catacatcaa aaaactaaat gtcactaaca
    14521 accgcatgtc tattttgttg ccataacgtc ctatcaaagg tgaaattaat agtggcatga
    14581 tgccgatggg cgcataagca agtccggccc atatcgcatt ataccccatc gtttcctgga
    14641 gtaactgcgg cataaggacg atcgctccag agtaaaataa atacgcgcat gtgatactca
    14701 caataccaat ggtgaagtta cgggacttaa acaaactgag atcaagaatc gggttctctg
    14761 aggtcgactc ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta
    14821 ttattgtact cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc
    14881 caccaacacc gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagttt
    14941 cagtttctct tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga
    15001 cattgattaa aaatatccaa ccccagctaa agttatcgca aatataaccg cccaatatcg
    15061 gcccacatat cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt
    15121 tttctggtgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
    15181 ttaatccctg aacgactcta aaaaatatca gcacatcaag attggtcgat aaactacaca
    15241 ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa taaccttaat tcgcctattc
    15301 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga
    15361 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
    15421 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtaa
    15481 cgcaccataa cgtcccaccg gttaatggtg ccggagtcga tttagtgatt gccattaaag
    15541 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
    15601 taaagcctta ctggtataag ccggcatgga cgtcacggtt gaagccagat caggcatctc
    15661 agcaatgtct tcgttcttcg tatcaatagt tgcagtcatc gataaaccaa tgcgtaaggg
    15721 gtgttccatg agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt
    15781 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
    15841 attgatccct gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat
    15901 attgactgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
    15961 ccacatttga cgtgccggta cgacagccat taacgattgt ccgggactca ctgtttcgcc
    16021 gacctgaaca ctccgctggg caatatagcc agtaaccgga ctccttatat ccgtacgttt
    16081 aagcgccaac caggcttctt tagttgcatc cgccgcttcc acgacttgtg gctgacggtt
    16141 taatggtgtg ttcattacta aagctttatt cgctttataa gcctggatag cggcattcag
    16201 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac
    16261 cccctgcttc gctaacggta ctcgacggtt ataatcttct aaagattgtt gatactgaat
    16321 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg
    16381 ccgaacaata tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt
    16441 atccagtgaa actaaaatgt caccttgtcg aacgtagttc gtatctttat gattaacgac
    16501 agtgacacta cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc
    16561 atctgtacta atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc
    16621 a
//
LOCUS       DAZFRJ010000096        16268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000096 DAZFRJ010000000
VERSION     DAZFRJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.096"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..397
                     /locus_tag="ACHKUN_002835"
     CDS             128..397
                     /locus_tag="ACHKUN_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6661060.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            366..1484
                     /locus_tag="ACHKUN_002836"
     CDS             366..1484
                     /locus_tag="ACHKUN_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6661061.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1651..2445
                     /gene="potC"
                     /locus_tag="ACHKUN_002837"
     CDS             1651..2445
                     /gene="potC"
                     /locus_tag="ACHKUN_002837"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO6661062.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2442..3488
                     /gene="potD"
                     /locus_tag="ACHKUN_002838"
     CDS             2442..3488
                     /gene="potD"
                     /locus_tag="ACHKUN_002838"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO6661063.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3546..4007
                     /locus_tag="ACHKUN_002839"
     CDS             3546..4007
                     /locus_tag="ACHKUN_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO6661064.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4004..4792
                     /locus_tag="ACHKUN_002840"
     CDS             4004..4792
                     /locus_tag="ACHKUN_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6661065.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(4931..5752)
                     /gene="cobB"
                     /locus_tag="ACHKUN_002841"
     CDS             complement(4931..5752)
                     /gene="cobB"
                     /locus_tag="ACHKUN_002841"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO6661066.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5768..6679)
                     /gene="nagK"
                     /locus_tag="ACHKUN_002842"
     CDS             complement(5768..6679)
                     /gene="nagK"
                     /locus_tag="ACHKUN_002842"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO6661067.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6708..7952)
                     /gene="lolE"
                     /locus_tag="ACHKUN_002843"
     CDS             complement(6708..7952)
                     /gene="lolE"
                     /locus_tag="ACHKUN_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO6661068.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(7952..8653)
                     /gene="lolD"
                     /locus_tag="ACHKUN_002844"
     CDS             complement(7952..8653)
                     /gene="lolD"
                     /locus_tag="ACHKUN_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO6661069.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8646..9845)
                     /gene="lolC"
                     /locus_tag="ACHKUN_002845"
     CDS             complement(8646..9845)
                     /gene="lolC"
                     /locus_tag="ACHKUN_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO6661070.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10107..11180
                     /locus_tag="ACHKUN_002846"
     CDS             10107..11180
                     /locus_tag="ACHKUN_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO6661071.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11308..14754
                     /gene="mfd"
                     /locus_tag="ACHKUN_002847"
     CDS             11308..14754
                     /gene="mfd"
                     /locus_tag="ACHKUN_002847"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO6661072.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            14898..15860
                     /gene="ldtC"
                     /locus_tag="ACHKUN_002848"
     CDS             14898..15860
                     /gene="ldtC"
                     /locus_tag="ACHKUN_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO6661073.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGSSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(15943..16200)
                     /gene="bhsA"
                     /locus_tag="ACHKUN_002849"
                     /gene_synonym="ycfR"
     CDS             complement(15943..16200)
                     /gene="bhsA"
                     /locus_tag="ACHKUN_002849"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO6661074.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4116 a         4183 c         4085 g         3884 t
ORIGIN      
        1 cgtcaccagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgaa tcaggctgat
       61 ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc gttccggagt
      121 gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa aacggttcag
      181 tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca tcgttccaag
      241 accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg ttgggcctgg
      301 tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga aggagatttt
      361 aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt tacgaaaagt
      421 ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact ggaaaatttc
      481 acggactcgg aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc aatcagcgaa
      541 tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg tcagcaacaa
      601 aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata caggacacgc
      661 gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaaacccga atggctgcat
      721 acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga gactttgcat
      781 taatactgga tgagtggctg gataaagaca tggtcagcac agcgagagta aatcgtggat
      841 tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct ggatggaatc
      901 ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc accatggaag
      961 actggcaaaa aatttacaat gcaacgcctg aaaaacactt tatccgtaac gcaatgcttc
     1021 ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt tcagatgtgt
     1081 ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg ttaccgctta
     1141 cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg tgccgagaca
     1201 gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccgaag ccgatgagta
     1261 aagacaacct gagcgactac tttgccaaag cacgggatct ggctgggata attccaccag
     1321 caggaaaaac tccgccaaca tttcatgaac aacgctctct atcagaacgg ctgtaccgtg
     1381 cacagggtat tgatacaaaa acattactag gacataaagt ccaggcaacc accgatcgct
     1441 ataacgatac tcgaggtcag gaatgggtta agttggttat ttgataaaca ccatatggaa
     1501 tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggcctagtt ttggggaaac
     1561 gttttggaga gattttggag aaaggaaaaa attgaataaa ttcaaaatcc tgaaagtgtc
     1621 ccgccttatg aataaaaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt
     1681 atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt
     1741 aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg
     1801 atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg
     1861 gcgacatttg ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt
     1921 cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc agatatcgtg
     1981 atggcgattt ctctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg
     2041 ctgctgttct cgcatatcac cttctgcctg ccgtttgtgg tggtgacggt gtattcgcgc
     2101 ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagtgaattt
     2161 accattctgc gaaaaatcat tctgccactg gcaatgcctg cggtggcggc gggctgggtg
     2221 ttaagcttta ccctgtcgat ggacgatgtg gtagtttctt cgttcgtcac cggaccaagt
     2281 tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt
     2341 aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt
     2401 attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc
     2461 acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca
     2521 acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca
     2581 ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg
     2641 ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg
     2701 tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag ttaacgaact
     2761 tcagcaatct cgatccagac atgctcaaca agccgtttga cccgaataac gattactcca
     2821 ttccgtatat ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat
     2881 ctgtcaccag ctgggccgat ctgtggaagc ctgagtacaa aggcagcctg ctgttgaccg
     2941 acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca
     3001 ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg
     3061 cagcgtttaa ctccgataac ccagctaacc cgtacatgga aggcgaagtt aacctcggca
     3121 tgatctggaa cggttctgct ttcgttgcac ggcaggcggg tacgccaatt gacgtggtgt
     3181 ggccgaaaga aggcggcatt ttctggatgg acagcctggc gatcccggca aatgccaaaa
     3241 acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg
     3301 ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagcccgg
     3361 aagtggcgaa cgataaaacg ctctacccgg atgctgaaac cattaaaaat ggtgaatggc
     3421 agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag
     3481 gacgttaatt cagcaagcgg gcggtggcat taccgcccgt tttttattta gcaaggatgt
     3541 ggctaatgag tcaggacagt aaagtgttat cccgcgcttt tctcggtatc ggtctgatat
     3601 tgctcgttat ttctgcaatc atcttttatt accattttac ttttaccaaa agcgcggttc
     3661 atacagaagg aatcattgtt gatgccgttt ggtacaacaa tcactcaaac gacgtcgatg
     3721 ataatggttc ctggtatcca gtggttgcct ttcgccctac gccagattac acgctaattt
     3781 tcaattcgaa tatcggtagt gatttttatg aagatagcga aggtgacaga gtcaatgtct
     3841 attatccgcc agggcatcca gaacaagccg aaataaacaa tccctgggtt aattttttta
     3901 aatggggctt tgttggcata gcaggcatta ttttcggtag tgtcggtctg ataatcacgc
     3961 tcccctccac caaaaaatct cgcaggaaac ggaagtcctg cccatgaaaa aattcattat
     4021 actgctcagt ttactgatac tcctgcctct ggtagcaacc agtaaaccat tgattccaat
     4081 aatgaagaca ctttttacgg atgttaccgg tactgtaccg gatgctgaag agatcgcacg
     4141 taaagccgag ctttttcgcc aacaaacggg aattgcaccg tttattgtga ttttacccga
     4201 cattaataat gaagccagtc tcagacaaaa tggtaaagcg atgctggcgc atgcgtcatc
     4261 ttcattgagt aatgtaaaag gaagtgttct gttactattt actacccgcg aaccacggtt
     4321 aattatgatc accaacggcc aggttgaaag tggtctggac gataaacatc tcggccttct
     4381 gatagaaaat cacacgctgg cttatttaaa tgcagatctc tggtatcagg gaatcaataa
     4441 tgcattggct gttctacaag cacagatatt aaaacaaccg acgccgccac tgacgtatta
     4501 tccgcatcca ggacaacagc atgaaaatgc tccccccggc agcaccaaca cattaggttt
     4561 tatcgcctgg gctgcgacgt ttatcttatt ttcctggatc ttttgctaca cgactcgttt
     4621 tatttatgcg ttaaaatttg cagtggcgat gactattgcc aatatgggtt atcaggcttt
     4681 atgtttgtat atcgataata gctttgcgat taccagaata tcgccactct gggcagggct
     4741 aattggtgtc tgtacgttta ttgccgcatt gttattgacg agtaaacgat aaaagaggcc
     4801 ggaaaagtgc gcgtgtatta tgccggatgg ggcgcgaacg ccttatccgg cccacaaaac
     4861 ccgcaaattc aattaattgc gtccccttgt aggcctgata agcgtagcgc atcaggcaat
     4921 gcttcctgct ttataatccc ttcagcaact tttcaacaaa ctcaggcacc acctggcttg
     4981 ccgggccgta atatttctcg gcaaattcat taccgacctg acttggttcg agattcagtt
     5041 ccacggtgtg cgcgccatgc agtttcgctt cgtgaacaaa cccagccgcc ggataaacat
     5101 gcccggaagt accaatggca atgaaaatat cagccatcga caacgccata taaatttcat
     5161 ccatgccgag tggcatttcg ccaaaccaaa cgacgtgcgg gcgcaagggt gccgggaact
     5221 ggcaacaatg gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac
     5281 tttgcgaaca acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg
     5341 cgcgttcatg caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagggcgt
     5401 cttgcagttt agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct
     5461 gtcgacgacg ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg
     5521 gcgttgccac atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac
     5581 gaatacctga ttccgcagaa atccctgccc ctgtcagtac gagtactctt ggtttttcca
     5641 ttgcttccgg caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc
     5701 ggcgtttatt tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct
     5761 ctgttgttta atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt
     5821 ggcgcgcgcg ttcaatgcgc ggaacacgag ctacaggtaa gagatgacga ggcagcctgt
     5881 ccgccagttg cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt
     5941 cagggtcaac aatggtcagg atatttccca gacaaaccgc taataaatcc agataacgct
     6001 caacgtgcgc ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct
     6061 gcaacggttg atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat
     6121 tttcaatgca gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca
     6181 tggttaacgc atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac
     6241 cggtaatcgg tttgccgttg aaaatcagcc cgccgccaac gccggtgccg agaatcaacc
     6301 ccatcaccag tggatattga gtaaattcat catcccaggc ttctgaaagg gcaaaacagt
     6361 tggcatcgtt atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg
     6421 gtttaccgct ggcagcgggg acattggcgg catacagcgt accatcttct gtttccggca
     6481 ttcccggaat accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta
     6541 ccagctcaca cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct
     6601 tttcccactg caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa
     6661 tatcaaaccc gtaatacatt gccgctcctt gttttaatgt actgccttta ctggccgcta
     6721 aggactcgcg cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga
     6781 ctcagcaaca atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc
     6841 aggaaatcaa taaaatagat atcgctggag aggaactgat gaccgatcaa cttttcaatc
     6901 cactcaataa tcggggtaag ttgcagtgaa accaccacac cgataatcac cccacacagg
     6961 ctgccgaaca gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct
     7021 ttcgccccca gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact
     7081 aaggtggaga cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata
     7141 atggcgcgga tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta
     7201 atataaacat agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa
     7261 acatccgtca ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg
     7321 gcatccgcca gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata
     7381 ccggcaacgt gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg
     7441 gggatcataa tcgacaccca atccccctgc ttcactttca gcgcatccgc cacgcctttg
     7501 ccgatgataa tttgctgttc gcccgcttta aaattgcgcc acgcatcgcc ctggacaaac
     7561 gagggtaatg cgctcagacg ctgttcctgt tgcgggttaa cgcccttcac ctggattgcg
     7621 cgaagattcg cgccactttc caccagcccg gtgaaattga tatacggcgc ggcagcggca
     7681 atacccggca ctttttgcac gtgatccagt gcttcctgcc agttagtcca cggctgatcc
     7741 accgcctcga tttcaccatg cggcaccacc gccagaatac ggttgttcag ttcgcgttca
     7801 aagccgttca tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg
     7861 gtagaaataa cggagatcag cgacaccatg ccgccgcgcc gccgtccgcg actaaaacgc
     7921 aggccaatta ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg
     7981 ccgtcagacg accatcacgc atttctaact ggcggctcat acgtttcgcc agttgcaggt
     8041 cgtgagtaac caccaggaag gcggtgccct gcaagcgatt caattcccca agcaactgga
     8101 agatgctgtc ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccaggc
     8161 gcgggttatt caccagcgca cgggcaatcg ccacacgctg gcgttcgccg ccagaaagtt
     8221 cagatgggcg gtgattcgca cgatgatcca gccccaccgc ttttaacatc tcaagtgcac
     8281 ggctgttgat ttcagcgggc tttttcttgc caatcagcag cggcatagcc acgttttcca
     8341 gggcggtaaa atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc
     8401 gcagctctgc tttcgccgcc gaagacagtt tgctcatcgg ttgaccgtta aagatcacat
     8461 cgccggaggt tggcgtatcc agcccgccca acaagtgcag caaggtgctt ttaccggaac
     8521 cagagctacc gacgatcgcc atcatttcac cttcgccgac gctgaaactg acattgtgca
     8581 gcacatcggt ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca
     8641 ggatcttatt cataacgtaa agcctcagcg ggttgagtgg cggcagcgcg ccatgaaggg
     8701 taaagcgtag acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa
     8761 ggttcgatag cgaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga
     8821 ttatttaact ggctggcaag cagtgcgcca agcgccgcac cgaggatcgc accgataatc
     8881 ccggcgctgg ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc
     8941 gtttgcagga tcgcgacttc gccctgcttc tccattacca tcagccctag tgaggtaata
     9001 atgttaaacg ccgcaacggc gacaatcagg ctcagcagca agcccatcat atttttttcc
     9061 atgcgtacgg cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct
     9121 tcaggcagtt tttgctgact taaagagtca actttcagcg gctcatccag ccacaaacgc
     9181 cagccggtaa tattgcctgc cggataacgc atcagacgcg aggcatcctc aatattcacc
     9241 agcatttcat agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg
     9301 cgctggcttg gaatacgccc catcggcgtg aactggctgg cagatggtac catcacgcgg
     9361 atttgatcac cgcgattaac gcctagctgt gaggcaagtt gttcgccgag gatgacatta
     9421 tatttccccg gctcgaggtc agtttgtttc acattgacca gatacggtgt aagtggatct
     9481 ttttgcgccg ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt
     9541 accacatcac cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt
     9601 tccgggagtt gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc
     9661 aggccaagga tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg
     9721 accagcgcca tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg
     9781 aagcgatccg ctgcacgccc acgcatgtaa cgcaggccaa taaatagagc gacaggttgg
     9841 tacatgaaat ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg
     9901 ggtaaacgtc tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa
     9961 tcggctaaat tgccatgttt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca
    10021 tccaatacag acagatcccc attacacctc gggtcataac ccgcaggatc aaccgcatgt
    10081 aaattaaaaa agagcgtgtt gtcctgatga aacaaaaaga gctatggatt aaccagatca
    10141 aagggttatg tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcatc
    10201 tgaccacttt tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc
    10261 tttaccttgc cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc
    10321 gctatatcga cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct
    10381 gggtgctggc gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg aaccagtggc
    10441 tggcacctga gcgcgattta agcaatgcct ccaatgccgc ttatgccgat tccaccggtg
    10501 agttcctgca cgggatgatc accgccagca ccagcttgtg gtatctgtat gcgttaattg
    10561 tctatttcgt ggtatgtaaa atttttagcc gtctggcgct gccactattc gccttgtttg
    10621 tactgctgag tgtggcggtt aatttcgttc ccacgccgtg gtggggaatg aacagtgtga
    10681 tccgcaattt gctttattac agccttggcg catggtttgg cgcaacaata atgacctgtg
    10741 ttaaagaggt gccgttgcgc cgccatctgc tgatggcttc tttgcttacc gttctggcgg
    10801 tcggtgcctg gttgtttact atctcgctgc tgttgtcgct ggtatcgatt gtggtgatca
    10861 tgaagctgtt ttatcaatac gagcaacgat tcggtatgcg ctccaccagc ctgctgaatg
    10921 tgattggctc caacaccatt gctatctaca ccacccatcg cattctggtt gaaatattca
    10981 gcttaactct gcttgcgcaa atgaacgcag cacgctggtc gccgcaagtc gaactgacac
    11041 tcctgctggt ttatcccttt gtcagtttgt tcatctgtac tgttgcgggc ttgctggtaa
    11101 gaaaactttc acagcgtgca ttcagcgatc tgttgttctc cccgccttca ctgcccgctg
    11161 ccgtcagtta ctctcgttaa accatcgccc cagccggggc gattcaattt gctaaaccat
    11221 gtcgattacg gataatgaac agcattgctt atcagagata tgcccccata tgttgaggca
    11281 tatcctaacg agaatctgac aaccgttatg cctgaacaat atcgttatac gctgcccgtc
    11341 aaagcgggtg agcagcgtct gctgggcgag ttaaccggcg cagcctgtgc aacgctggta
    11401 gcggaaattg ccgaacgtca cgccggtccg gtggtactca ttgcaccaga tatgcaaaat
    11461 gctctgcgtt tgcatgatga aatcagccag ttcaccgatc agatggtgat gaatctggcg
    11521 gactgggaaa ctcttcccta cgacagtttt tcgcctcatc aggacattat ctcctcgcgc
    11581 ctttccaccc tttaccagct accgacgatg cagcgtggcg tactgattgt tccggtgaat
    11641 acgcttatgc agcgcgtttg cccacacagt tttctccacg gtcatgcgct ggtgatgaaa
    11701 aaaggtcagc gcctgtcacg agatgcatta cgaacccaac tggacagcgc cggttatcgc
    11761 catgttgacc aggtgatgga gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc
    11821 ttcccgatgg ggagtgagct gccttatcgt cttgatttct ttgatgatga aatcgacagc
    11881 ctgcgggtgt ttgacgtcga cagccagcgc acgctggagg aagtagaagc gatcaatctg
    11941 ctgcccgcgc acgaatttcc gaccgataaa gcggcaattg aactgttccg cagccagtgg
    12001 cgcgatacct tcgaagtgaa gcgcgatccg gaacatattt accagcaagt gagtaaaggc
    12061 acattacctg ccgggatcga gtactggcag ccattgttct tcagcgaacc actgccgccg
    12121 ctgttcagtt atttccctgc caataccttg ctcgtgaata ctggcgatct ggaaaccagt
    12181 gccgaacgtt tccaggctga cacgctggcg cgttttgaga atcgcggcgt cgatccgatg
    12241 cgcccgctgt tgccaccaca atcgctctgg ctgcgggtgg acgagctctt ctcagagctg
    12301 aaaaactggc cccgggtgca gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc
    12361 aatttaggtt tccagaaact gccagacctg gccgttcagg cacaacaaaa agcgccgctg
    12421 gatgcgctgc gtaagttcct cgagactttc gacggtccgg tggtgttctc ggtagaaagt
    12481 gaaggtcgcc gtgaagcgct gggtgaactg ctcgcacgaa ttaaaattgc tccgcaacgc
    12541 attatgcgtc ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg cgctgccgaa
    12601 catggttttg tcgataaggt gcgtaatctg gcgctgatct gcgaaagcga tctgctcggt
    12661 gaacgtgttg cccgccgtcg tcaggattct cgccgcacca tcaaccccga tacactgatc
    12721 cgtaaccttg cggagctgca tattggtcag ccggtggtcc atctggagca cggtgtcggg
    12781 cgctatgccg gaatgaccac gctcgaagcg ggcggcatta ctggcgagta tttgatgctc
    12841 acctatgcca acgacgccaa actgtatgtt ccggtgtcgt cactgcatct gattagccgt
    12901 tacgcaggtg gcgcggaaga aaacgccccg ctgcataaac ttggcggcga tgcgtggtca
    12961 cgcgcgcggc agaaagcggc ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc
    13021 tacgcgcaac gcgccgccaa agagggcttc gcgtttaaac acgatcgtga gcagtatcag
    13081 ttgttctgcg acagcttccc gtttgaaacc acgccggatc aggcgcaggc cattaatgcg
    13141 gtacttagcg acatgtgtca gccgctggca atggatcgtc tggtgtgcgg cgatgttggc
    13201 tttggtaaaa cagaagtggc gatgcgcgcc gctttcctgg cagtagataa ccacaagcag
    13261 gtggcggtgt tggtgcctac cacccttctc gcgcagcagc attacgacaa cttccgcgac
    13321 cgtttcgcca actggccggt acgtatcgaa atgatctccc gtttccgcag cgccaaagag
    13381 cagacgcaaa tccttgcgga agtggcggaa gggaaaatcg atattctgat cggtacgcac
    13441 aaactgctgc aaagtgacgt caagtttaaa gatttaggcc tgctgattgt cgatgaagaa
    13501 caccgcttcg gggtgcgtca taaagagcgt attaaagcga tgcgcgcgaa cgtggatatt
    13561 ctgacgctta ctgcaacgcc gatcccacgt acgctgaata tggcaatgag cggaatgcgt
    13621 gacctgtcga ttatcgccac gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt
    13681 gagtatgaca gcctggtggt ccgggaggcg atcctgcgtg aaattttgcg cggaggacag
    13741 gtttattatc tctacaatga tgtggaaaac attcagaaag ccgccgaacg gctggcagaa
    13801 ctggtgccag aagcgcggat cgccatcggt cacgggcaga tgcgcgagcg cgaactggaa
    13861 cgggtgatga atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac aaccattatc
    13921 gaaaccggga tcgacatccc gacagccaac accattatca ttgaacgcgc ggatcacttc
    13981 ggtctggcgc agctgcacca gttacgcggt cgcgtcggac gttcacatca tcaggcatat
    14041 gcatggttgc tgacgccgca tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa
    14101 gcgattgcct cgctggaaga tctcggggca ggttttgcgc tggcaacgca cgatctggag
    14161 attcgcggcg cgggtgaact gcttggcgaa gaacaaagcg gctcaatgga aaccatcggt
    14221 ttctcgctgt atatggagtt gctggaaaac gccgtcgatg cactgaaagc cggacgcgag
    14281 ccgtcgctgg aagatctcac cagccagcaa acagaagtcg agctgcggat gccgtcgcta
    14341 ttgccagatg atttcattcc tgacgtgaat acgcgtttgt cgttctataa acgtattgcc
    14401 agcgccaaaa cggaaaacga actggaagag atcaaagtcg agcttatcga tcgcttcggc
    14461 ctgctgccgg atccggcgcg taccctgctg gatgttgccc gtctgcgcca gcaagcgcag
    14521 aaactgggga tcaggaagct ggaaggtaat gagaaaggcg gggtgatcga atttgccgag
    14581 aagaatcacg ttaatccggc ctggttgatt ggtttgctgc aaaaacagcc gcagcattac
    14641 cgccttgatg gtccgacgcg cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt
    14701 atcgaatggg tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc ttaatggtaa
    14761 atcgggggcg tttctgcgcc cccatctgca acatttacaa attctttgca cttccctgca
    14821 ctatccgaca ctgtcaccat ccataattca ggcttatctg tttattacaa taaccttata
    14881 tttattatgg atttttggtg atgatcaaaa cgcgtttttc tcgctggcta acgtttttta
    14941 cgttcgccgc tgccgtggcg ctggcgctac cggcaaaagc caacacctgg ccgctgccgc
    15001 cagcgggcag tcgtctggtt ggcgaaaaca aatttcatgt ggtggaaaat gacggtagtt
    15061 ctctggaagc catcgccaaa aaatataacg tcggctttct cgctctgtta caggctaacc
    15121 ctggcgttga tccttacgta ccgcgcgcgg gtagcgtgtt aacgatcccg ttgcaaaccc
    15181 tacttccaga tgcgccgcgc gaaggcattg tgatcaatat tgcggagctg cgtctctatt
    15241 actacccgcc gggtaaaaat tcggtaaccg tgtatccaat aggtattggt cagttaggtg
    15301 gtgacacgct gacaccgaca atggtgacca ccgtttcaga caaacgtgca aacccaacct
    15361 ggacgccaac ggcaaacatc cgcgcccgtt ataaagcaca gggaattgag ttgcctgcgg
    15421 tagtgccggc tggaccggat aacccaatgg gccatcatgc gattcgtctg gcggcctatg
    15481 gcggcgttta tttgcttcac ggtacgaacg ccgatttcgg cattggcatg cgggtaagtt
    15541 ctggctgtat tcgtctgcgg gatgacgata tcaaaacact ctttagccag gtcaccccag
    15601 gcaccaaagt gaatatcatc aacactccga taaaagtctc tgccgaacca aacggtgcgc
    15661 gtctggttga agtacatcag ccgctgtcag agaagattga tgacgatccg cagctgctgc
    15721 caattacgct gaatagcgca atgcaatcat ttaaagatgc agcacaaact gacgctgaag
    15781 tgatgcaaca tgtgatggat gtccgttccg ggatgccggt ggatgtccgc cgtcatcaag
    15841 tgagcccaca aactctgtaa atcctggata aaaaaaatcc ccgcgacacg gcatgtcgcg
    15901 gggaaaaaat cagcagtagc aggcattaat gagggttaat gcttatttat aaattactgc
    15961 tgttccatgg agggtattcg gaccggttac agaagtaata cggaaagatt ttgcgcccat
    16021 ctcatccgct ttttgcgcca gctgctcttc cagcgatccc agatttgtcc ccgcgttagc
    16081 actgattgta ccgacttttt gttggccttc tggcgttgac tgaacttcga cagccgcaaa
    16141 gctggcaaat gacatggagc ttaaaatcgc cgcagcgatg agggttttta cgtttttcat
    16201 aatagtggcc ttatgcagat gaatgacgtc gcaagcaatt acttaacgat cgataactaa
    16261 atgataga
//
LOCUS       DAZFRJ010000097        16194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000097 DAZFRJ010000000
VERSION     DAZFRJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.097"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(211..552)
                     /locus_tag="ACHKUN_002850"
     CDS             complement(211..552)
                     /locus_tag="ACHKUN_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO6661075.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(565..1437)
                     /gene="copD"
                     /locus_tag="ACHKUN_002851"
     CDS             complement(565..1437)
                     /gene="copD"
                     /locus_tag="ACHKUN_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO6661076.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(1441..1815)
                     /gene="yobA"
                     /locus_tag="ACHKUN_002852"
     CDS             complement(1441..1815)
                     /gene="yobA"
                     /locus_tag="ACHKUN_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO6661077.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            1954..2184
                     /gene="holE"
                     /locus_tag="ACHKUN_002853"
     CDS             1954..2184
                     /gene="holE"
                     /locus_tag="ACHKUN_002853"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO6661078.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2286..2942
                     /gene="yobB"
                     /locus_tag="ACHKUN_002854"
     CDS             2286..2942
                     /gene="yobB"
                     /locus_tag="ACHKUN_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO6661079.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            2966..3628
                     /gene="exoX"
                     /locus_tag="ACHKUN_002855"
     CDS             2966..3628
                     /gene="exoX"
                     /locus_tag="ACHKUN_002855"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO6661080.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3625..5685)
                     /gene="ptrB"
                     /locus_tag="ACHKUN_002856"
     CDS             complement(3625..5685)
                     /gene="ptrB"
                     /locus_tag="ACHKUN_002856"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO6661081.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCIDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(5894..6553)
                     /locus_tag="ACHKUN_002857"
     CDS             complement(5894..6553)
                     /locus_tag="ACHKUN_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO6661082.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(6880..7236)
                     /gene="yebF"
                     /locus_tag="ACHKUN_002858"
     CDS             complement(6880..7236)
                     /gene="yebF"
                     /locus_tag="ACHKUN_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO6661083.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(7303..7593)
                     /gene="yebG"
                     /locus_tag="ACHKUN_002859"
     CDS             complement(7303..7593)
                     /gene="yebG"
                     /locus_tag="ACHKUN_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO6661084.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDSADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            7727..8905
                     /gene="purT"
                     /locus_tag="ACHKUN_002860"
     CDS             7727..8905
                     /gene="purT"
                     /locus_tag="ACHKUN_002860"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO6661085.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(8961..9602)
                     /gene="kdgA"
                     /locus_tag="ACHKUN_002861"
                     /gene_synonym="eda"
     CDS             complement(8961..9602)
                     /gene="kdgA"
                     /locus_tag="ACHKUN_002861"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6661086.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(9639..11450)
                     /gene="edd"
                     /locus_tag="ACHKUN_002862"
     CDS             complement(9639..11450)
                     /gene="edd"
                     /locus_tag="ACHKUN_002862"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO6661087.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(11685..13160)
                     /gene="zwf"
                     /locus_tag="ACHKUN_002863"
     CDS             complement(11685..13160)
                     /gene="zwf"
                     /locus_tag="ACHKUN_002863"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO6661088.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            13498..14367
                     /gene="hexR"
                     /locus_tag="ACHKUN_002864"
                     /gene_synonym="yebK"
     CDS             13498..14367
                     /gene="hexR"
                     /locus_tag="ACHKUN_002864"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO6661089.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14495..15937
                     /gene="pyk"
                     /locus_tag="ACHKUN_002865"
     CDS             14495..15937
                     /gene="pyk"
                     /locus_tag="ACHKUN_002865"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO6661090.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(15997..>16194)
                     /locus_tag="ACHKUN_002866"
     CDS             complement(15997..>16194)
                     /locus_tag="ACHKUN_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HFO6661091.1"
                     /translation="YITINDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYL
                     DLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         3978 a         4172 c         3999 g         4045 t
ORIGIN      
        1 ggagagagcc gtgcgctaaa agttggcatt aatgcaggct tagttgcctt gccctttaag
       61 aatagatgac gacgccaggt tttccagttt gcgtgcaaaa tggtcaataa aaagcgtggt
      121 ggtcatcagc tgaaatgtta aaaaccgccc gttctggtga aagaactgag gcggtttttt
      181 tattggaaat caaaaggcta ttttaggtaa ttaacagagt ttttcagctc gttctataaa
      241 cggtgccaga ctcatttttt cgccgggatt gttaggatca tcaatctgaa tcaccgaaat
      301 gggttgggct ttagtcttcc cactggcaac ttccttttgt gcgatatcgt ttaaaggata
      361 ctgcacgagg gtacttgggt taatgacata caaagcatta cccggtctgc aagtcagcat
      421 cacctcttcg cgattaaacg cccatttgtc tttacccact tcaaaacgac tgacggtaat
      481 cacctgcggt gcagccagcg ccgctgcaga actggtgagt aacagaaacg tcagaatact
      541 ttttttcatc attttcgtaa tccatcaaaa aggttcgaga gtcgcaagca ggctgatgat
      601 caccagcact accgcaccta tcgcccattc gagtttagtc atccactcaa accagggtgc
      661 cgcacgatct tcatcttgcc tcatgcgcgg tacaagaaca taacgattag ccagcgcaat
      721 gaccaccata atcatcacca ggatggcttt gagcaataag agttggcccc agtaagtcgt
      781 aagcgtgggg ggaaatccgg taatcaacaa tgcattaagc acgccgcttg ccagtacgcc
      841 gatcaccgca aaatgcccgc accaggaaaa acgcatcagc gcctgaatgg cctggtgtcg
      901 ccaacggcct ttgatgagtt gcatacacca gagcaccggc agtaatcccc caaaccacgc
      961 cgccgcacaa atcaggtgaa tcgcgtgatt agtctgatgg attttcgccg ttaccccttc
     1021 attcagtgtc gcatgcccga ttcccgccag cagaataaat tgcgcggtag tgagcataaa
     1081 cagcaaacgt ggcatattcc gcggttgcat aagggcgacg atcaacgtta cgagggcgag
     1141 aacaatttgc cataaccaga cgccaccaaa ctgcgtttgc aaaacagcct gccaaatgtt
     1201 tggcgaaaat acatctgccc atcccgttcc catcaacccg ccctgaacgg cgagcattgc
     1261 agtggcactg atcaaactcc agacggcagc gtgctgttgt aaacgcagaa aacgcttcgc
     1321 cagcaagcga cgaatcgtta agggggccag ccaggcaccg tacattgcga agccaaagac
     1381 cagcatcagc gaggtgaaat ggataaatcg cagcgcgatc caggtaaacg ccagcatgat
     1441 ttatttcacg ctaaaggtgt agtgtccttt cgttttatgc ccgtccaccg aaacaacatg
     1501 ccagtcaacg atgtaggttc cgggtttcag cgaatcggcc agtggaacaa tcagttgctt
     1561 ttggtcctgc tcatttcgct tcgccggtaa tgttttaata ttttcgtttt ttggccccgt
     1621 gatttttgca ccactgaatc ctgtttcaac accttccgag aagtttaaag tgattgcctg
     1681 cggtgcagct gtcacttgcg cgtttgccgc aggatactga tgcgttaaat gcgcatgtgc
     1741 ccagacggaa ggtgtcacca acgaagtggt caggattgcc agcgcgtagc gaagggagcg
     1801 tgcagttgaa gtcatattat ctattccttt ttgtaataac ttttttacag agcataacct
     1861 tgtctaatgt ctgagtcgag gatcatcaat tccggcttgc catcctggct cactcttagt
     1921 aacttttgcc cgcgaatgat gaggagatta agaatgctga agaatctggc taaactggat
     1981 caaacagaaa tggataaagt gaatgtcgat ttggcggcgg ccggggtggc atttaaagaa
     2041 cgctacaata tgccggtgat cgctgaagcg gttgaacgtg aacagcctga acatttgcgc
     2101 agctggtttc gcgagcggct tattgcccac cgtttggctt cggtcaatct gtcacgttta
     2161 ccttacgagc ccaaacttaa ataaaactta tacagagtta cactttctta cataacgcct
     2221 gctaaattat gagtattttc taaaccgcac ccataatttg cagtcatttt gaaaaggaag
     2281 tcattatgtc gttctggaaa gttgcagcag cgcaatatga gcctcgtaaa acctcactca
     2341 ccgaacaggt agctcatcat ctggagtttg tgagggccgc cgccaggcaa caatgtcagc
     2401 tgttagtttt tccatcgctt tctttactgg ggtgtgatta ttcgcgacgt gcccttcctg
     2461 ccccacccga tctctcactg ttagacccgc tttgttatgc cgcaacgacc tggcgaatga
     2521 ccatcattgc cggccttccc gttgaatata acgatcgctt tattcgtgga attgcggtgt
     2581 tcgcaccgtg gcgaaaaacg cctgggatct accatcagag tcatggtgca tgtctgggtc
     2641 gtcgttccag aaccatcacg gtggtcgatg aacaaccgca aggtatggat atggacccga
     2701 cctgttcact gtttactaca gggcaatgtc tgggagaacc cgacctgctg gcgtcggccc
     2761 gtcgcttaca atttttttca catcagtact cgattgccgt gctgatggca aatgcccgtg
     2821 gtaacagtgc actatgggat gaatatggtc gtctcatcgt tcgcgccgat cgtggttcat
     2881 tattgttagt cggtcagcgt tcatcacagg gttggcaagg cgatatcatt ccattacgct
     2941 aggctttttc ggcctggagc atgccatgtt gcgcattatc gatacagaaa cctgcggttt
     3001 gcagggaggg atcgttgaga ttgcctctgt tgatgtcatt gacggaaaaa tcgtcaaccc
     3061 catgagccac ctggtgcgcc ccgatcgtcc tattagtcca caagcgatgg cgattcatcg
     3121 catcaccgaa gccatggtcg ccgataaacc gtggattgaa gatgtgatcc cacactatta
     3181 cggtagtgaa tggtatgtcg cgcataacgc cagctttgac cgccgcgtac tgcctgagat
     3241 gcccggtgag tggatttgca ctatgaaact ggcccgtcgt ttgtggcctg ggatcaagta
     3301 cagcaatatg gcgttatata aaacacgcaa gctcaatgta cagacgccgc cgggcctgca
     3361 tcatcaccgc gcgttgtatg actgttatat caccgccgcg ttgcttatcg atattatgaa
     3421 cacctccggc tggacggcag aacagatggc cgatatcacc ggacgtccgt cgttgatgac
     3481 gaccttcacc tttggcaaat accgtggcaa agcggtttcc gacgttgccg aacgcgatcc
     3541 gggctatctg cgctggttat ttaataacct ggacagcatg agcccggagc tgcgtttaac
     3601 actgaaacat tatctggaaa atacttagtc cgcaggcgta gcgggtaatg ttccctgcgc
     3661 cagcgcgacc agaaaagcat attccatcgc tacgccttcg tacgatttaa agcgaccaga
     3721 tttaccgcca tggcctgagt ccatgtcgat acagagcaat aaaagatggt catcggtttt
     3781 cagctcgcgc aatttagcga cccattttgc cggttcccaa tattgcacct gagaatcgtg
     3841 caaaccggtc gttaccagta aatgcggata agcctgtgcg gtgacgttgt catacgggct
     3901 gtagcttttc atgtactcgt aatattgcgg atcctgcgga ttaccccact cttcaaactc
     3961 accagtggta agaggaattg attcatcaag catcgttgtt acaacatcaa caaacggtac
     4021 ctgggcgata acgccgtgga ataattccgg gcgttgatta attgcaacgc ccatcaacat
     4081 gccccccgca ctcccgccca tcgcataaca aagcgaagga gagccatagc ccagttttaa
     4141 caatgcatcg caggcatcaa gataatcatt aaacgtattt ttcttcttca gaaattttcc
     4201 gtcttcgtac cattgttgcc ccagctcacc accgccgcga acatggacaa tggcgtagac
     4261 aaagccacga tctaacaaac tcaagcggct aaaactgaaa tcggcatcaa tacttgcgcc
     4321 gtaagaacca tagccataca ccagcaacgg gttgtgtcct ttgcgaaaat gtttgcgatg
     4381 gtagaccaac gaaaccggaa cttcgacgcc atcacgggcg actatccaca ggtgttcact
     4441 gcggtaattc gccgcatcaa aaccgggcac ttctgtttgt tttaatacac gacgctcacc
     4501 ggtatccata tccagttcaa acaaagtgtc tggtgtggtc atggaagaat aaccataacg
     4561 caatcgcgcg gtttcaggtt ctggattgta ggcaatccag gtcacatagg ctggatcatc
     4621 aaaggcaata ccaatgactt cccgggtctt gcggttaatt tggcgcaaac tggttaaccc
     4681 gcgctgacgc tcttcaacca ccagccagtc ggtaaacagc gtaaaccctt ccagcatgat
     4741 gttttcgcgt ggcggaatta actcttccca ctgttgctca tcacgcatac gggtacggta
     4801 taagccaaag tttttgccgt ggcggttgga acgtagataa aaacgatgct ggtagtgatc
     4861 aaggctgtat tcgtgatctt tgcggcgcgg cagaaaaaca aacggctcgg catcggccat
     4921 ttccgcgtcc agcaggcgaa cttcactggt ggtggcgctg gccaaatgaa tgactacata
     4981 gtgcttcgag gtcgttttat gcaggctgac gtaataggta tcgtcttttt cttcgtagat
     5041 cagtttatct tgcgatgctg gcgtaccgat ggcgtgacgc cagacctgat aaggcagcag
     5101 cgtcaccgga tgcttgcgaa catagtagaa agtccaggag tcatttgccc agacaaagct
     5161 gggttcaacg ttatccagca gttccgggta ccagttacca gtttccagat tacgaaaacg
     5221 aatgccgtac tggcgtcggg aaagaaaatc ttctgccagc gccataatgg tgttatcggg
     5281 cgtaatcgcc attccgccca tcgaataaaa ctcactatga gctgcgcgct tattggcatc
     5341 gagcaatgtt tcccactcat cccactcttc actgaatgcc gattgacgct ggtagatagc
     5401 atattcacag cctggttcat aaatatgccg atagcggtag ccatttttga tgtagggcgc
     5461 agaaacttct cgttgcggaa tgcggtcgat gatttccttt aagatgcgat cctgcaaggc
     5521 ttgttgtgag gccatcaccc gatgaccgta actattttct tgttgcaggt agtccaggac
     5581 ttctggctga gaacgcgtat cgtcccgcag ccagtagtaa ttatcgatgc gcgtatcgcc
     5641 atgaagcgtc atggcgtggg gaatgcgggc ggcttttggt agcatgttat tgttctttct
     5701 ggttgaaaca tcttataagg gtggcaaaac tcaccgggga tgcaagcgaa acaggggagt
     5761 cattgcttag atgatgacag gtaatggcgc ggatatcgaa tgttatgcag acagagaaaa
     5821 tcagcctgtt caaatggctg tgcgattctg gatagcccga aatagtcaac ttcaggctat
     5881 ccagagagcg gaattattcc gccaaagtgc gtttttgctg ttcgagatcg cgttcaatgc
     5941 cgtcacgaac atcctggggg attttcagcg cgtcacccag tgcattcagg taactgcgtt
     6001 ccataaaatg gtcaatatca atagccgcgc aactcaggaa atagatttcc agcgcctctt
     6061 cttcattgcg gaccccggta gccaggcgtt gtggatccag cggttgttcg attgcctgct
     6121 caatgagtac acgcccctgc tcttccacgc cggcttcacg caattgctgg tcgattgccg
     6181 cacgttcttt ggcatcaata tgaccatcac ttttagcggc aaagaccagc gcaaggatca
     6241 aacgttctgt acgctcatcc agcggcgtac tttgcgcgcc aaactgcggt tcgtcctgat
     6301 gcgccgcgcg aattttatct ttgtatttat tccacagcac cgtacccgct accgctccgc
     6361 cgccaaccag taacgcgttg gtgccatatt tcgtaagaag tttacgtgct gatttatttg
     6421 cgaccagcag cccagccagt ccgcctaatg cgcctggcac taacagtttg cccaatccct
     6481 gatccgcaga cgaggaggta gaagaactgc tttgcccaag cagggattgc agttgattta
     6541 accagttagc catatttgct cctcaataac catttttagt gcctgtagca tagcggatga
     6601 ggatgtcaga aaatgtatat cgagacaaaa gatgcgcaaa taagcggggc ggtaggccgt
     6661 tattcaaaga aattcgcgcc aggtaaggta tttactgtat atcaggtgat aagggtattt
     6721 taggtgaaag tagtttgtcg ttttgagtgg cattgtttga tgctgattgc ccgatgcgac
     6781 gctgttgcgt cttctcaggc ctacgattgt ggtgcgttct ggggcggaca aggcactcgc
     6841 gccgcatccg ccaacggtca atcgaattat cagaagccat taacgccgct gatattccgc
     6901 cattcccgct ttgcagtcca cgctgacctg gtaatgaata tcggcacttt taccacgcac
     6961 ggttagcggt actgaccatt tatcatcttt accctgaatg tcctgcaaac tgacccaagc
     7021 cacgggatcg gcctgaccga caattttttg atcatctgcc caacgcgcca cgcgattttg
     7081 ttgataatca cgttttacgc tcgcggcaat tccggcagca tccagacctt cacactttgg
     7141 gaaagtgacc gacttgctgg tttcattatt ggcagcgaaa actgatgcgc aggcagaaac
     7201 caacaacagc cctaaaaacg cccctctttt tttcatgttt ttctccatag cacaatgatt
     7261 caggagaaag catggtacaa attgtcagga gcgcaagttg cttcaggccg cgtgtgaggc
     7321 atcttcctct tcggattcag caccgacgac ctgtggggac ggtaatttgc cggttttcag
     7381 aatggtgctc agcacatctt tttgttctgc cagccatagc gaaagggctt cacgttgctc
     7441 gtcttccatt tgcactggag aatttgtcag ccaggtgtca agcagatccg ccgaatcaag
     7501 cattttatca taagcatcgg cttccttttt gctggtaaac gacattttct cttcgccctc
     7561 acgaatgact acgtatttaa cttcaaccgc catttgcagc ctctcataat aactgtgatt
     7621 ttatacagta tatttctttt cggttgagaa atcaacatca gcaataaaga cacacgcaaa
     7681 cgttttcgtt tatactgcgc gcggaattaa tcaggggata ttcgttatga cgttattagg
     7741 cactgcgctg cgtccggcag caactcgcgt gatgttatta ggctccggtg aactgggtaa
     7801 agaagtggca atcgagtgtc agcgtctcgg cgtagaggtg attgccgtcg atcgctatgc
     7861 cgacgcacca gccatgcatg tcgcgcatcg ctcccatgtc attaatatgc ttgatggtga
     7921 tgcattacgc cgtgtggttg aactggaaaa accacattat atcgtgccgg agatcgaagc
     7981 tattgccacc gatatgctga tccaacttga agaggaagga ctgaatgttg tcccctgcgc
     8041 tcgcgcaacg aaattaacga tgaatcgcga gggtatccgt cgcctggcgg cagaacagct
     8101 gcagctgccc acttccactt atcgttttgc cgatagcgaa agccttttcc gcgaggcggt
     8161 tgccgccatt ggctatccct gcattgtaaa accggtgatg agctcttccg gcaaggggca
     8221 gacgtttatt cgctctgcag agcaacttgc tcaggcatgg gaatacgctc agcaaggcgg
     8281 tcgcgccgga gcgggccgcg taattgttga aggcgtcgtt aagtttgact tcgaaattac
     8341 cctgctaacc gtcagcgcgg tggatggcgt ccatttctgt gcaccagtag gtcatcgcca
     8401 ggaagatggc gactaccgtg aatcctggca accacagcaa atgagcccgc ttgcccttga
     8461 acgtgcgcag gagattgccc gtaaagtggt gctggcactg ggcggttatg gtctgtttgg
     8521 tgtcgagctg tttgtctgtg gtgatgaggt gattttcagt gaggtctccc ctcgtccaca
     8581 tgataccggg atggtgacgt taatttctca agatctctca gagtttgccc tgcatgtacg
     8641 tgccttcctc ggacttccgg ttggcgggat ccgccagtat ggtcctgcag cttctgccgt
     8701 tatcctgcca caactgacca gtcagaatgt cacgtttgat aatgtgcaga atgccgtagg
     8761 cgcaggtttg cagattcgtt tatttggtaa gccggcaatt aatggcagcc gtcgtctggg
     8821 ggtggcactg gctactgcag agagtgttgt tgacgccatt aaacgcgcga agcacgccgc
     8881 cggacaggta aaagtacagg gttaaacccg gcaaaaaaac gctacaaaaa tgcccgatcc
     8941 gcgatcgggc attttgactt ttacagctta gcgccttcta cagcttcacg cgccagctta
     9001 gtaatacggt cgtaatcgcc cgcttccagc gcatctgccg gaaccagcca ggaaccaccg
     9061 atgcacagca cgcttttcag cgccaggtag tcacggtagt tagccggaga aataccacct
     9121 gtcgggcaga aacggacctg ggagaacgga cccgcgatcg cctgcagggc tttcacgccg
     9181 ccgttagctt cagccgggaa gaatttgaac tctttcaaac cgtagtccat acccagcatc
     9241 agttcggaaa cagtgctgat ccccggaatc agaggaatag tcccttcggt agcagctttc
     9301 agcagcggct cggtcagacc cgggctaatt gcgaactgtg cacccgcttc ggtgacttct
     9361 gccagctgct gtggattcag caccgtaccg gcacccacaa tcgcttcagg cacttctttg
     9421 gcgatagcac ggatagcgtc aactgcacac tcggtacgca gagtcacttc cagaacgcgc
     9481 accccaccag caaccaacgc ttttgccatc ggcactgcgt gttccagttt ttttaccacg
     9541 ataaccggta caaccgggcc ggtggtcagg attgattctg cacttgtttt ccagtttttc
     9601 atcagagttt tctctcgcct gattacaaat ttgtcgtctt aaaaagtgat acaggttgcg
     9661 ccctgttcgg caccggacag tttttcacgc aaggcgctga ataattcacg tcctgtcccc
     9721 acgcgtgacg cgctcaggtc aggaatgtgc ggttcgcgag cagccagttc cgcttcgtct
     9781 accagcagcg tcagttcgcc tgtctgtcca ttcacacgaa tgatgtcccc gtcgcgcact
     9841 tttgccagca gcccgccatc gtaggcttct ggtgttacgt ggatagctga cggcacttta
     9901 cctgaagcgc cggagagtcg tccatcggta actaacgcaa ttttgaaaca ccggtccaat
     9961 aatacaccaa gtggcggcat gagtttatgt aattctggca ttccgttcgc ttttggcccc
    10021 tgatgacgga caacaacgac acaatcgcgg tccagcaaac ccgcttcaaa ggccggcata
    10081 acgtcatgct ggctttcaaa aacaaccgct ggcgcttcaa tcacctggtt ctcaaccggc
    10141 acggcagagg ttttcataac cgcacggccc aggttaccgc ttaacacttt tgtcccacca
    10201 tgatgagaga aaggttgttc gaaggaagcg atcacattgc tgtcgagtga tttttccgcc
    10261 ccttcccgcc agtccagttc accattattc agccatggtt caagggtata acgagacaga
    10321 ccaaagcctg ccaccgtatt gacatcttca tgcagcaggc ctgctttgag cagttcacgc
    10381 accagaaccg gtacgccacc tgccgcctgg aagtggttaa tatcggccgg accgttcggg
    10441 tagagacgtg ccatcagcgg tacaacatca gaaaggtcag agaagtcatc ccagttaatc
    10501 tgaataccgg ccgcgcgcgc catcgccacc aggtgcatgg tgtggttagt ggaaccaccg
    10561 gtcgccagca gtgcaacgat accgttcacc accactttct catcgatcat cttaccgatc
    10621 ggcatccatt cattaccatt accggtcatg cgtgtaacct gacgcgcagc ggcagcggtc
    10681 aaagcatcgc gcagcggaga atccggatga acaaaagaag agcctggcaa ctgcatcccc
    10741 ataaattcca ccaccatctg gttggtgttg gcagtaccgt agaaagtaca tgttcccggc
    10801 gcatggtaag acgcggcttc tgactccagt aaggccatgc ggtccacttt accttcggca
    10861 taaagctggc gaatacgcac tttttcttta tttggcaaac cgcttgccat cggtccagac
    10921 ggcacaaaca ccgcaggcaa atgaccaaac gacagggctg ccatcgtcag acccgggaca
    10981 atcttgtcgc acacaccgag gaacagagca ccatcaaaca tgttatggga cagcccgacc
    11041 gccgcagaca tcgctatcac ttcgcggctt agcagcgaca attccattcc atcctgcccc
    11101 tgggtgacac catcacacat cgccggaaca ccgcccgcaa cctgaccaac cgcattcgct
    11161 tcatgcaggg ctttacgaat gatttctgga tagtgttcat aaggctggtg cgcggagagc
    11221 atgtcgttat aggaggtgat gatggcgata ttgttacgca acatgctttt caaagaggct
    11281 ttgtcttctg gctggcaggc agcgaaaccg tgtgccaggt taccgcatgc caactgcgaa
    11341 cgatgaacgg tcgaagtttt cgcttgttct atccgggcga gataagcaga gcgagtctcg
    11401 cgcgaacgtt caatgattcg atttgttacg cgtaacaatt gtggattcat aaaggctcct
    11461 gaaattgagt tgtcagagca ggatgattca caacgcgttt cattcagagg atttatgact
    11521 gaaacgcctg taaccggagc tcatagggca aaaacgtttc agtcagtgta ataaaaaaag
    11581 cctcgtgggt gaatccgcac gaggcctgaa agtgtaaaaa ttgttctaca atctgtgcaa
    11641 gatcatgtta ccggtaaaat aaccataaag gataagcgca gatattactc aaactcattc
    11701 caggaacgac catcacgggt aatcatcgcc accgaggcaa cgggtcccca ggttccggcc
    11761 tgatacggtt tcggcgcatc attgtccatc gcccacgcct cagtaatgga gtctacccat
    11821 ttccaggctt cttccacttc gtcgcgacga acaaacagtg cctgaatacc acgcatggtt
    11881 tccagcagca aacgttcata ggcatccgcc agatgcgtct gattaaaggt ttctgaatag
    11941 ctcagatcca gcttggtgat ttgcaggtta tgtttgtggt caaggccagg aactttattc
    12001 agtacctgga tatccacgcc ttcatcaggt tgcagacgga tagtcagttt attctgcggc
    12061 agatcctgcc acgattcttt aaacagattc agttcaggtg ttttgaaata gaccacgact
    12121 tcagaacatt tggtcggcag acgtttacca gtacgcaggt agaatggcac accggcccag
    12181 cgccagttat caatgtcgac gcggatcgct acgaaggttt ctgtattgct gctcttgttc
    12241 gcgccctctt cttccagata tcccggcact tttttgccct gggcgaagcc cgcagtatat
    12301 tgcccgcgta cggttttttc gcgtacgttg gagcggtcga tgcggcgcag agacttcagt
    12361 actttcactt tttcatcgcg gatgctgtct gcgctcaggt cagacggcgg agacatcgca
    12421 atcatgcaga gaatttgcag caggtggttt tggatcatat cgcgcatctg accggcttta
    12481 tcaaaatagc cccagcgccc ttcgatcccc acttcttctg ccacggtaat ctcaacatga
    12541 tcaatggtgc gattgtccca gttattcaca aacagggagt tagcaaaacg cagcgccaac
    12601 aggttcagca ccgtttcttt accaagatag tggtcgatac ggtaaacctg gcactcctcg
    12661 aagtattcgc caacctgatc attgatttcc tgcgaggtcg ccagcgacgt ccccagcggt
    12721 ttctccatga ctacgcgtgc cggtttagca ttcagtttag cctcgccaag ccctttgcaa
    12781 attgcgccaa aagtgctggg cggcatggca aagtagttaa tggtgatacg atttttttga
    12841 tccagcatcg cgccgagacg gctgaatgca gcagtgtcat tgacatcgag attacaaaaa
    12901 tccagacgtg cactcagggt gtcccataaa ccttcatcaa tggtttcttt catgaaagtt
    12961 tcgagcgcct cgcggacaac tttggtatat gccgctttat cccagtcagc acgccctacg
    13021 ccgataatcc gggtgtccgg gttgagctga ccggcttttt ccagttgata cagggaaggc
    13081 agcaatttac gacgcgcaag gtcgcctttc gcgccgaaaa tgaccaggtc acaggcctgg
    13141 gctgtttgcg ttaccgccat gtcattctcc ttaagttaac taacccggta cttaagccag
    13201 ggtatacttg taattttctt acggtgcact gtactgcttt tacgagcttg cgaaaactgt
    13261 aaacgcttat ccacccgtgc gattacggta aaagcgcgca aagtgcggca aaactgataa
    13321 aaaaatcatc gttttccatt gggtaaaaat ctgacactga tcatgttatg aaaaaaaata
    13381 acaacttttt tatctgcttt tgtcattaac acagcacaca ggcgtaatat ctgacaaaac
    13441 tacatttcga tttctttcag tgcggaaatc gtcattaccc gtgagtttct ttacatcatg
    13501 aatatgctgg aaaaaatcca gtctcagctg gaacatttga gcaaatcaga gcgcaaagtt
    13561 gccgaggtca ttctggcttc gcccgataac gcgatccatt cgagtattgc tgctctggcg
    13621 ctggaagcca atgttagcga accgacggtg aatcgtttct gtcgcagcat ggacacgcgc
    13681 ggttttcctg attttaaact tcatctggca caaagtctgg cgaatggcac tccctatgtt
    13741 aatcgcaatg tcaatgaaga tgacagcgtt gaatcataca cagggaaaat atttgagtcc
    13801 gcaatggcaa cgcttgatca tgtccgtcat tcactggata aatctgccat caaccgcgcc
    13861 gtcgacttgc tcactcaggc aaaaaaaatc gcctttttcg gattaggctc ttcagccgcc
    13921 gttgcccacg atgcgatgaa taagttcttt cgttttaatg ttccggtggt gtactccgat
    13981 gatatcgtgc tgcaacgcat gagttgtatg aattgtagcg acggagacgt ggtggtgctg
    14041 atttctcaca ctggaagaac aaaaaatctg gtcgagctgg cgcagctggc tcgcgaaaac
    14101 gacgccatgg tgattgccct cacctctgcg ggtaccccgc tcgcccggga agcaacgctg
    14161 gcaattaccc tcgacgtacc ggaagatact gacatttata tgcccatggt ttctcgactt
    14221 gcacagctga ccgtgataga tgtgctggcg acaggattta ctttgcgacg cggtgcaaaa
    14281 ttcagagata acttgaagcg ggtcaaggaa gcgctgaagg aatcgcgttt tgataagcag
    14341 ttacttaatt taagtgacga tcgctaaaaa cgactgtcac tgtcctaatc ttatacgaca
    14401 tccgaatgag attaatttat cgccatcgcg gcgttatttc attcggattt catgttcaag
    14461 caacacctgg ttgtttcagt caacggagta ttacatgtcc agaaggcttc gcagaacaaa
    14521 aatcgttacc acgttaggcc cagcaacaga tcgcgataat aatcttgaaa aagttatcgc
    14581 ggcgggtgcc aacgttgtac gtatgaactt ttctcacggc tcgcctgaag atcacaaaat
    14641 gcgcgcggat aaagttcgtg agattgccgc aaaactgggg cgtcatgtgg ctattctggg
    14701 tgacctccag gggcccaaaa tccgtgtatc cacctttaaa gaaggcaaag ttttcctcaa
    14761 tattggggat aaattcctgc tcgacgccaa cctgggtaaa ggtgaaggcg acaaagaaaa
    14821 agtcggtatc gactacaaag gcctgcctgc tgacgttgtg cctggcgaca tcctgctgct
    14881 ggacgatggt cgcgttcagt taaaagtact ggaagttcag ggcatgaaag tgttcaccga
    14941 agtgaccgtc ggtggtcccc tctccaacaa taaaggtatc aacaaacttg gcggcggttt
    15001 gtcggctgaa gcgctgaccg aaaaagacaa agcagacatt aagactgcgg cgttgattgg
    15061 cgtagattac ctggctgtct ccttcccacg ctgcggcgaa gatctgaact atgcccgtcg
    15121 cctggcacgc gatgcaggat gtgatgcgaa aattgttgcc aaggttgaac gtgcggaagc
    15181 cgtttgcagc caggatgcaa tggatgacat catcctcgcc tctgacgtgg taatggttgc
    15241 acgcggcgac ctcggtgtgg aaattggcga cccggaactg gtcggcattc agaaagcgtt
    15301 gatccgtcgt gcgcgtcagc ttaaccgcgc ggtaatcacg gcgacccaga tgatggagtc
    15361 aatgattact aacccgatgc cgacgcgtgc agaagtcatg gacgtagcaa acgccgttct
    15421 ggatggtact gacgctgtga tgctgtctgc agaaactgcc gctgggcagt atccgtcaga
    15481 aactgttgca gccatggcgc gcgtttgcct gggtgcggaa aaaatcccga gcatcaacgt
    15541 ttctaaacac cgtctggacg ttcagttcga caatgtggaa gaagctattg ccatgtcagc
    15601 aatgtacgca gctaaccacc tgaaaggcgt tacggcgatt attaccatga ccgaatcggg
    15661 tcgtaccgcg ctgatgacct cccgtatcag ctctggtcta ccaattttcg ccatgtcgcg
    15721 ccatgaacgt acgctgaacc tgactgctct ctatcgtggc gttacgccgg tgcactttga
    15781 tagcgctaat gacggcgtag cagctgccag cgaagcggtt aatctgctac gcgataaagg
    15841 ttacttgatg tctggtgacc tggtgattgt cacccagggc gacgtgatga gtaccgtggg
    15901 ttctactaat accacgcgta ttttaacggt agagtaatct cacctgcccc ggccgctgat
    15961 gaccggggta tttagcaaca cataaattag gactactcat tcaatccaat cataatcaca
    16021 gccccctgta aaatgaatac agggatagcc tgaatggaga tctaaatagc ttgcaagacg
    16081 tcgcataccc gcttcctctg caacactata tccaataact gccatcggta agccactatc
    16141 tttggagtat tgtgtctgca cccatgtttt aattgaatcg ttaatcgtaa tata
//
LOCUS       DAZFRJ010000098        16077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000098 DAZFRJ010000000
VERSION     DAZFRJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.098"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..155)
                     /gene="yojO"
                     /locus_tag="ACHKUN_002867"
     CDS             complement(<1..155)
                     /gene="yojO"
                     /locus_tag="ACHKUN_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO6661092.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSL"
     gene            264..758
                     /gene="napF"
                     /locus_tag="ACHKUN_002868"
     CDS             264..758
                     /gene="napF"
                     /locus_tag="ACHKUN_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO6661093.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            748..1011
                     /gene="napD"
                     /locus_tag="ACHKUN_002869"
     CDS             748..1011
                     /gene="napD"
                     /locus_tag="ACHKUN_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO6661094.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1008..3494
                     /gene="napA"
                     /locus_tag="ACHKUN_002870"
     CDS             1008..3494
                     /gene="napA"
                     /locus_tag="ACHKUN_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO6661095.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3501..4196
                     /gene="napG"
                     /locus_tag="ACHKUN_002871"
     CDS             3501..4196
                     /gene="napG"
                     /locus_tag="ACHKUN_002871"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO6661096.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4183..5046
                     /gene="napH"
                     /locus_tag="ACHKUN_002872"
     CDS             4183..5046
                     /gene="napH"
                     /locus_tag="ACHKUN_002872"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO6661097.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5043..5492
                     /gene="napB"
                     /locus_tag="ACHKUN_002873"
     CDS             5043..5492
                     /gene="napB"
                     /locus_tag="ACHKUN_002873"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO6661098.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5502..6104
                     /gene="napC"
                     /locus_tag="ACHKUN_002874"
     CDS             5502..6104
                     /gene="napC"
                     /locus_tag="ACHKUN_002874"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO6661099.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6117..6740
                     /gene="ccmA"
                     /locus_tag="ACHKUN_002875"
     CDS             6117..6740
                     /gene="ccmA"
                     /locus_tag="ACHKUN_002875"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO6661100.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6737..7399
                     /gene="ccmB"
                     /locus_tag="ACHKUN_002876"
     CDS             6737..7399
                     /gene="ccmB"
                     /locus_tag="ACHKUN_002876"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO6661101.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7441..8178
                     /gene="ccmC"
                     /locus_tag="ACHKUN_002877"
     CDS             7441..8178
                     /gene="ccmC"
                     /locus_tag="ACHKUN_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO6661102.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8175..8384
                     /gene="ccmD"
                     /locus_tag="ACHKUN_002878"
     CDS             8175..8384
                     /gene="ccmD"
                     /locus_tag="ACHKUN_002878"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO6661103.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8381..8860
                     /gene="ccmE"
                     /locus_tag="ACHKUN_002879"
     CDS             8381..8860
                     /gene="ccmE"
                     /locus_tag="ACHKUN_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO6661104.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8857..10800
                     /gene="ccmF"
                     /locus_tag="ACHKUN_002880"
     CDS             8857..10800
                     /gene="ccmF"
                     /locus_tag="ACHKUN_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO6661105.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10797..11354
                     /gene="dsbE"
                     /locus_tag="ACHKUN_002881"
     CDS             10797..11354
                     /gene="dsbE"
                     /locus_tag="ACHKUN_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO6661106.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11351..12403
                     /gene="ccmH"
                     /locus_tag="ACHKUN_002882"
     CDS             11351..12403
                     /gene="ccmH"
                     /locus_tag="ACHKUN_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO6661107.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(12438..13085)
                     /gene="narP"
                     /locus_tag="ACHKUN_002883"
     CDS             complement(12438..13085)
                     /gene="narP"
                     /locus_tag="ACHKUN_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO6661108.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13405..15996
                     /gene="yejO"
                     /locus_tag="ACHKUN_002884"
                     /pseudo
     CDS             13405..15996
                     /gene="yejO"
                     /locus_tag="ACHKUN_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3586 a         3948 c         4679 g         3864 t
ORIGIN      
        1 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
       61 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
      121 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
      181 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
      241 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
      301 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
      361 ttctgaccca ttgcacccgc tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
      421 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
      481 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
      541 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
      601 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
      661 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
      721 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
      781 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
      841 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
      901 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
      961 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     1021 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     1081 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     1141 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
     1201 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
     1261 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
     1321 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
     1381 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa aggaccggaa tcgatcggta
     1441 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgttcaaag
     1501 cgggcttccg ttcgaacaac atcgacccga acgcacgtca ctgtatggcg tcggcagtag
     1561 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
     1621 aggctgacgc gtttgtgctg tggggcgcta acatggcgga gatgcacccg atcctctggt
     1681 cacgcatcac taaccgtcgt ctctctaacc agaacgtcac cgtggcggtg ctttctacct
     1741 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
     1801 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
     1861 tcttcagcaa gcacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
     1921 cgacccatcc gctggaaaaa gcagcgaaga atccgggttc tgacgcctcc gaaccgatga
     1981 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaact gccgaaatga
     2041 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
     2101 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggccaaca
     2161 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
     2221 tctccctgac cgggcagcct tccgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
     2281 accgtctgcc tgcggacatg gtggtgacta acgagaaaca ccgtgatatt tgcgagaaga
     2341 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
     2401 accgtgcact gaaagacggc aagctgaatg tttactggac catgtgtacc aacaacatgc
     2461 aggccgggcc gaacattaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
     2521 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc agccgacttg atcctgccga
     2581 ccgcaatgtg ggtagagaaa gagggcgctt acggtaacgc cgaacgccgt actcagttct
     2641 ggcgtcagca ggtacaggcg ccaggcgaag cgaaatcgga tctctggcag ttagtccagt
     2701 tctcccgccg cttcaaaact gaagaagtat ggccggaaga gctgctggcg aagaaaccgg
     2761 aactgcgtgg caaaacgctg tacgaagttc tgtatgccac ccccgaagtg agcaaatacc
     2821 ctgtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
     2881 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctgg
     2941 caccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
     3001 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
     3061 agttctacgg taaaccggat ggcaaagcgg tgatcttcgc actgccgttc gaaccggcgg
     3121 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
     3181 ggcacaccgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
     3241 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
     3301 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
     3361 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactga
     3421 cgctggatgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
     3481 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
     3541 ttctgcgcga tgtcgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
     3601 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
     3661 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
     3721 acaccttaaa actggcgacg ctggcctctg gtctgtcggc gggcacgcca tattttgtcg
     3781 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccaagcg
     3841 gtgcgctgga tcgtgagatt gaatcgatcg acgacgcgcg gatggggctg gcggtactgg
     3901 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
     3961 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
     4021 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggttgcggt aagtgcgaaa
     4081 aagtatgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
     4141 agttaggtca ccattaccgc ttcggttggc tggaggggaa caatggcaaa tcgtaaacgt
     4201 gacgccgggc gcgaggcgct ggagaaaaaa ggctggtggc gcagtcaccg ttggctggtg
     4261 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc atggtttggt
     4321 gtgtggatct tgcacggcaa ctacagcagt agcctgttat tcgacaccgt tccgctgacc
     4381 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
     4441 accggtgcgg tgattatcac cgtgctctac gccctggcgg gaaagcgatt attttgcagc
     4501 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
     4561 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
     4621 ctgggatcgg ccttaactgg cacgctcatc tgggaatgga tcaaccccgt atctctgatg
     4681 gggcgcagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
     4741 tttgatctac tggtcgttga acacggctgg tgcgggtaca tttgtccggt tggcgcgctg
     4801 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccaccgatcg gcagaaatgt
     4861 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
     4921 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
     4981 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
     5041 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
     5101 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
     5161 ccgggacaca ggaaggggcc attcggatgc caaaagagca ggaacggatg ccgctgaact
     5221 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
     5281 ccaaccgctg cttgcagtgc cacggtgtcg aaagttatcg caccactggc gcgccgcgta
     5341 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
     5401 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
     5461 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
     5521 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
     5581 ggacgctgct gttgatcggt tttgttggcg gcatcgtctt ctggggtggc tttaacaccg
     5641 ggatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgcaacacgg
     5701 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
     5761 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
     5821 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
     5881 atcgtctgac gatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
     5941 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
     6001 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
     6061 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
     6121 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
     6181 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
     6241 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
     6301 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
     6361 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
     6421 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
     6481 aagatattcc tgtaaatcag ctctcggccg ggcaacaacg ccgcgtcgct ttagcgcgtc
     6541 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
     6601 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
     6661 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
     6721 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
     6781 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
     6841 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
     6901 ggttgctgca ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
     6961 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
     7021 gaaggtgatg gcgcactgga tggtaagcgg tctgccgtta ctcattcttt cgccactggt
     7081 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
     7141 gggaacgcct acgcttggct ttctcggtgc accgggcgtg gcgctgacag tgggacttaa
     7201 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
     7261 cgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
     7321 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
     7381 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
     7441 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
     7501 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
     7561 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
     7621 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
     7681 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
     7741 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
     7801 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctttt tttgtatgtg
     7861 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
     7921 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
     7981 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
     8041 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
     8101 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
     8161 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
     8221 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
     8281 tcgtgcactc ggtgatgcag catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
     8341 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
     8401 ccgcttgtgg attgcctgtg ctgttctggc agggttggca ctgactatcg gtctggtgct
     8461 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
     8521 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat
     8581 gccgggtagc gtgcagcgcg atcccaattc gctaaaagta actttcacca tttacgacgc
     8641 tgaaggttca gtagatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
     8701 gggcgttgtg gtgcagggcg aactggaaaa aggcaatcat atcctcgcaa aagaagtgct
     8761 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagccaatca
     8821 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggtaacggg
     8881 ctgctgtgtc tggcgctagg aattgcgctg ctgctgtccg tgtatccgct atggggcgta
     8941 gcgcgcggag atgcgcgcat gatggcgtct tcccgcttgt ttgcctggct gctgtttatg
     9001 tctgtggctg gcgcatttct ggtactggtc aatgccttcg tggtcaacga cttcaccgtc
     9061 acctatgttg ccagcaactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
     9121 tggggcgcgc atgaaggctc gctcctgctg tgggtgctgc tgatgagcgg ctggaccttt
     9181 gcggtagcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
     9241 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccgttctca
     9301 cgcacgttgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggatccg
     9361 gggctgatct tccatccgcc tctgctctat atggggtacg tggggttctc ggtggcgttt
     9421 gcttttgcca ttgcttcttt gttgtgcggg cgtctggaca gcacttatgc gcgttttact
     9481 cgtccgtgga cgctggcggc gtggatcttc ttgacgctcg gcattgtgct cggttccgca
     9541 tgggcctatt acgaactcgg ctggggcggc tggtggttct gggatccggt agaaaacgcc
     9601 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
     9661 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
     9721 ctggggactt tcctggtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcctctgat
     9781 ccggcacgcg gtatgtttat cctcgccttt atggtactgg tgattggtgg ttcgctgctg
     9841 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
     9901 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtattactg
     9961 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    10021 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctgct gcttggtgtc
    10081 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    10141 gccttcatct caacgctggt gctgtcgctg ctgctgccgt ggctgttcga aagcaaagtt
    10201 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgctgtgct ggcaattgcg
    10261 gaagctgcgc tgcgtatttc acgtggcacg aaaaccacct tcagttattg gggaatggtg
    10321 gcggctcacc tggggctggc agtgacaatt gttggtattg cctttagcca gaactatagc
    10381 gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc
    10441 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    10501 ggcgtaacgc gcgatggcaa gccggaaacg gtgctgtatg cggaaaaacg ttattacaac
    10561 actgccgggt cgatgatgac cgaagcggca attgacggcg gcatcacgcg tgacctgtac
    10621 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggccg tgcgtcttta ctacaaacca
    10681 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact actgtgtctg
    10741 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggccgtatga
    10801 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    10861 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    10921 gcaagcctgt gccgaagttt cgtctcgaat cactggacaa tccggggcag ttttatcagg
    10981 cggatgtgct gactcagggc aaaccagtac tgcttaacgt ctgggcgacc tggtgtccga
    11041 cctgccgtgc ggaacatcaa tatctgaatc agctttcagc gcagggcatt cgcgtggtcg
    11101 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    11161 cttacgcgct aagcctgttt gatggcgacg gcatgttagg gctggatctc ggtgtctatg
    11221 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    11281 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    11341 aggccgcaca atgaggtttt tattgggcgt gctgatgctg atgatctccg gctcagcgct
    11401 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    11461 cactgaagaa ctgcgctgcc cgaaatgcca gaataacagc attgccgatt ccaactcgat
    11521 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaagaa
    11581 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    11641 aacgccgctg accgtgctgc tgtgggtgct gccagtagtg gctattggca ttggcggttg
    11701 ggtcatttac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    11761 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    11821 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    11881 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagccga
    11941 tccgctcaac gaagaagaga tgtcgcgtct tgcgctgggg atgcgtactc aactgcaaaa
    12001 aaatccggga gatatagaag gctggattat gttgggccgc gttggcatgg cgctgggtaa
    12061 cgctagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagcga
    12121 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    12181 cggcggcgaa ctgctgcgcc agttggtgag aagcgaccac agcaatatcc gtgtgttaag
    12241 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    12301 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    12361 cgcgcaggcg atgcaacatt tgtcgccgca ggagaataaa taaaaagagg cccgatggtg
    12421 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggctgcc
    12481 acgcgtgagc ggacattgag tttgcgcagc agattgcgaa tatgtacttt tactgtctgc
    12541 tctgaaatat tcaacaccga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    12601 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    12661 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctcca
    12721 gcacgaattg cttccagcaa tacttccggg tcgctgtctt tcaacagata accgtctgcg
    12781 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatctg atacggtcag gataataatt
    12841 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    12901 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    12961 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    13021 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    13081 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    13141 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    13201 cttgtcgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    13261 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    13321 aaaactgact cccggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    13381 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    13441 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    13501 gagtatggtg agacagttga tggtgttgtc ctggaaaaag atatccagct ggtttatggg
    13561 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    13621 agtaataata ctgaaattaa cggagggtat cagtacattg aaatgaatgg cgccgcagaa
    13681 tactcagtat taaatgacgg ttatcaaatt gttcaaatgg gtggcgcggc aaaccagact
    13741 acgctcaata atggtgtgct acaggtttat ggcgcagcga atgataccac gattaaaggc
    13801 gggcgcttaa tcgttgaaaa agatgggggg gccgtctttg tcgctatcga aaagggagga
    13861 ctactggagg ttaaagaggg gggattagcg attgcggtag atcagaaagc aggcggtgct
    13921 attaaaacaa ccacgcgggc catggaggta ttcggaacaa accgtctcgg tcagttcgat
    13981 atcaagaatg gtattgctaa taatatgttg ttggaaaacg gcggaagttt gcgagttgaa
    14041 gaaaatgact tcgcttataa taccactgta gatagtggcg gcttactgga ggttatggat
    14101 ggcgggactg taactggcgt tgataaaaaa gcaggcggaa aattaattgt ctcaacgaat
    14161 gcgctggaag tgagtggtcc aaacagtaaa ggccaattta gtataaaaga tggtgtgtca
    14221 aaaaattatg aactggatga tggttccggg ctcattgtta tggaggacac gcaggccatt
    14281 gatactatcc ttgatgagca tgccactatg caatcgctgg gaaaggatac tggtacgaaa
    14341 gtgcaggcaa atgcggtata tgatctcggt cgatcatatc agaatggaag tatcacgtat
    14401 tcctcaaaag ccatctctga aaatatggtt atcaacaatg gccgcgctaa cgtctgggct
    14461 ggcacaatgg ttaacgtttc agtcagaggg aatgatggca ttcttgaggt catgaagccg
    14521 caaataaatt atgcacccgc aatgttggtg ggtaaggtag tggtttctga gggcgcttct
    14581 tttagaacgc atagtgccgt ggataccagc aaagcggacg tttcgctcga aaatagcgta
    14641 tggaccatca ttgccgatat cactacgacg aaccaaaaca ccctcctcaa cttagccaac
    14701 cttgcgatgt ctgacgcaaa tgtgattatg atggatgagc cagtgactcg ttcatcagtg
    14761 acggcaagtg cggaaaattt cattacgttg accaccaata ccctgtcggg aaacggcaat
    14821 ttttatatgc gtaccgatat ggctaatcat cagagcgatc agctcaacgt caccggtcag
    14881 gcaacaggtg atttcaaaat attcgtgacg gacaccggtg ccagcccggc agcaggagat
    14941 agccttacac tggtaacaac gggcggcggt gatgctgcat ttacgttggg caatgccgga
    15001 ggcgttgttg atatcggtac gtatgaatat accttgctgg ataatggcaa ccatagctgg
    15061 agtttggcag agaatcgcgc gcaaattacc ccttcaacca ctgatgtgct gaatatggcg
    15121 gcagcacaac cgctggtatt tgatgcagaa ctggacaccg tgcgtgagcg tcttggtagc
    15181 gtaaaaggcg ttaattacga tacggcgatg tggagttcgg caattaacac ccgcaacaac
    15241 gtgaccactg atgcgggcgc tggctttgag caaacattga ccgggctgac gctcggtatc
    15301 gatagccgtt tctcccgtga agaaagcagc acaattcgcg gcttgttctt tggttactct
    15361 cattctgata ttggttttga tcgcggcggc aaaggcaatg tcgatagcta taccctgggg
    15421 gcttatgccg gttgggagca tcagaacggt gcctatgttg atggagtggt gaaagttgac
    15481 cgttttgcca acaccatcca tggcaagatg agtaatgggg caacagcgtt tggcgattac
    15541 aatagtaacg gcgcgggtgc tcatgtcgag agcgggttcc gttgggttga cggattgtgg
    15601 agtgttagac cctatctggc ctttaccggc tttaccacag atggtcagga ctacacgtta
    15661 tcaaacggca tgcgcgcgga tgtgggaaat acccggatat tacgcgctga agcgggaacg
    15721 gcggtaagct atcacatgga cctgcaaaac ggtacgacgc tggaaccctg actgaaagcc
    15781 gccgtgcgtc aggaatacgc cgattctaac caggtgaaag ttaatgacga tggcaaattt
    15841 aataatgatg tggctggaac ccgtggcgtt tatcaggctg ggataaggtc atcgtttacc
    15901 ccgacgttaa gcggtcattt gtcagtcagc tatggcaatg gcgcaggggt agaatcgccg
    15961 tggaatactc aggcgggtgt ggtctggacg ttctgataac aaaaaataaa caggctgtga
    16021 tgtgtcacgg tctgtttatc gaattaattg cagatataaa aaaaaccaac cgtaaag
//
LOCUS       DAZFRJ010000099        15896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000099 DAZFRJ010000000
VERSION     DAZFRJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.099"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(248..1510)
                     /locus_tag="ACHKUN_002885"
     CDS             complement(248..1510)
                     /locus_tag="ACHKUN_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6661109.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
     gene            complement(1674..1758)
                     /locus_tag="ACHKUN_002886"
     tRNA            complement(1674..1758)
                     /locus_tag="ACHKUN_002886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1722..1724),aa:Leu,seq:caa)
     gene            1954..2973
                     /gene="ahr"
                     /locus_tag="ACHKUN_002887"
     CDS             1954..2973
                     /gene="ahr"
                     /locus_tag="ACHKUN_002887"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO6661110.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            3103..4605
                     /gene="yjgR"
                     /locus_tag="ACHKUN_002888"
     CDS             3103..4605
                     /gene="yjgR"
                     /locus_tag="ACHKUN_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO6661111.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4724..5806)
                     /gene="lptG"
                     /locus_tag="ACHKUN_002889"
     CDS             complement(4724..5806)
                     /gene="lptG"
                     /locus_tag="ACHKUN_002889"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6661112.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5806..6906)
                     /gene="lptF"
                     /locus_tag="ACHKUN_002890"
     CDS             complement(5806..6906)
                     /gene="lptF"
                     /locus_tag="ACHKUN_002890"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6661113.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6969..7019)
                     /gene="ytgA"
                     /locus_tag="ACHKUN_002891"
     CDS             complement(6969..7019)
                     /gene="ytgA"
                     /locus_tag="ACHKUN_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO6661114.1"
                     /translation="MSIIILATISTTQENK"
     gene            7173..8684
                     /gene="pepA"
                     /locus_tag="ACHKUN_002892"
     CDS             7173..8684
                     /gene="pepA"
                     /locus_tag="ACHKUN_002892"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO6661115.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9038..9481
                     /gene="holC"
                     /locus_tag="ACHKUN_002893"
     CDS             9038..9481
                     /gene="holC"
                     /locus_tag="ACHKUN_002893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO6661116.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9481..12336
                     /gene="valS"
                     /locus_tag="ACHKUN_002894"
     CDS             9481..12336
                     /gene="valS"
                     /locus_tag="ACHKUN_002894"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO6661117.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12390..13586)
                     /gene="yjgN"
                     /locus_tag="ACHKUN_002895"
                     /pseudo
     CDS             complement(12390..13586)
                     /gene="yjgN"
                     /locus_tag="ACHKUN_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            13779..14282
                     /gene="yjgM"
                     /locus_tag="ACHKUN_002896"
     CDS             13779..14282
                     /gene="yjgM"
                     /locus_tag="ACHKUN_002896"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6661118.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14328..14744)
                     /gene="rraB"
                     /locus_tag="ACHKUN_002897"
     CDS             complement(14328..14744)
                     /gene="rraB"
                     /locus_tag="ACHKUN_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO6661119.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14906..>15896
                     /gene="argF"
                     /locus_tag="ACHKUN_002898"
     CDS             14906..>15896
                     /gene="argF"
                     /locus_tag="ACHKUN_002898"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO6661120.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVA"
BASE COUNT         4073 a         4026 c         4111 g         3686 t
ORIGIN      
        1 atgataaaac cacaccgact aaaactgtaa tgaacagagt tagtcggctg gtttctataa
       61 caaaagccat accgtttgaa cactttttgt tttttcacta tcatctcaga atggccattg
      121 cacctcgctc taaaataatg ccccttggtt gaggttttcc cgcattttaa aagagcattt
      181 caatttatta ttatataatt ctctcacaca attaccaaat tagttaatgt ataatatttc
      241 agttacatta tttccccatg aatttagcaa attcataagg tgtaacagag ttatttcgat
      301 tggcctcaag aaaatcagcc caccattgaa ccattaatct acgttcgtct aaatgctcag
      361 aggtatgaat atatgctgca cgcacattgt tacgctctgt atgactcaac tgccgttcta
      421 tcgcgtcatc gctccataag cctgattcac caagcgcgcc acgcgccatc gtcctaaatc
      481 catgcccaca gacctcagtt ttcgtgtcat accccatcgc acgcaaggcg ctatttactg
      541 tgttctcact cataactttg gccgcgtcgt gatcccctgg aaaaagtaac tctttatcgc
      601 cactaatctg cttgagttgc tctaacaaaa ccatcgcctg tcgactaaga ggaacgatat
      661 gctcctcttt catcttcatg ccacgatacg agtaacgcac gcctttaatt tcttctcgct
      721 ttgcaggtat acgccaaaga gatttatcga agtcgaactc atcccatcgc gcgaaacgta
      781 actcactgga acgtacaaaa gttagtaagg aaagctcgac cgcgatcctt gtcattacac
      841 gaccacgata tgcagcaaga cgtgcaagaa actcagagaa gcggctagag ggtaaagctg
      901 gatagtgtcg tgctttggtt gtagataacg caccggccat gtcactggct ggattagagt
      961 cgatgtaatc gttctgtacg gcataacgca taatggccgt gacgcgctgt tgcaggcgct
     1021 gagcgacatc gtgtttgcca ctggcatcaa cttttttaat cggggctaac aggtggctgg
     1081 ttttaagctg ccgaatgtcg gacgaaccga tatgaggaaa aatataaagc tcaagatagc
     1141 gcagaatgcg tgatcgatgg tcttcactcc agcgcttatt actggcatgc cattcacgag
     1201 caattgtttc gaaagtgtat gcgcccgaat tctcagcctg agcttctttc tgctcggctt
     1261 ttgggtcaat gccctgcact aagagctttt tagcctcgtc acgctttgct cttgcctgag
     1321 caagtgtcac catgggccaa acaccaaaag cgaggcgatc ctcttttttg tcagaggggc
     1381 gtctgtattt catgcgccag tatttagaac ccttagccga aacctcgaga tacaaaccgc
     1441 cgccatcggc cattttgtag gttttctctt ttggctttgc tgtctcgacc tgtctggcgt
     1501 tgagcttcat ttgggggcac atttctaatc gaagttaaga tgcccccaat tatgccccca
     1561 atagcatccg gatttcaacg gactagctcg gacagctcag gacagaaaaa ccgctacagg
     1621 cattgatttt aaagggtttg ctggattttc tcggatggtc ttggaagtac taatggtgcc
     1681 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1741 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1801 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1861 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1921 acttcccaaa caacaccaga gaaggaccca aaaatgtcga tgataaaaag ctatgccgca
     1981 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     2041 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     2101 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgtg
     2161 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     2221 ggctggacgg cgcgtagctg tggtcactgc gacacctgta ttagcggtaa tcagatcaac
     2281 tgcgagcaag gtgcggtgcc gacgattatg aatcgcggtg gctttgccga gaagttgcgt
     2341 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2401 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2461 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2521 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2581 gcgatgggta ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2641 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2701 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2761 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2821 ccttatgagc tgcgtaagct gatgcgtttt gccgcccgca gcaaggttgc gccgaccacc
     2881 gaactgttcc cgatgtcgaa aattaacgac gccatccagc atgtacgcga tggtaaagcg
     2941 cgctaccgcg tggtcctgaa agccgacttc tgatcccttt gccgggagcc tgtcgcccgg
     3001 cacttaaatc tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcac
     3061 agccgttgcc tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta
     3121 attgcccgca cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg
     3181 ctgattactg gcgcaacggg gacgggtaaa actgtcacgc tgcaaaaact ggcggagtct
     3241 ctatcggaaa tcggcgtgcc ggtgtttatg gccgatgtga aaggcgatct gaccggtatc
     3301 gcgcaggcag gaacggcgtc ggaaaaactg ctcgcaaggc ttaaaaatat cggcgtaaat
     3361 gactggcaac cgcatgctaa tccggtggtg gtgtgggata tctttggcga gaaaggccat
     3421 ccggtgcggg cgacggtttc ggatctgggg ccgctgttgc tggcgcggct gttgaatctc
     3481 aacgatgtgc aatctggcgt gctgaatatc atcttccgaa ttgctgacga tcaggggctg
     3541 ttactgctcg actttaaaga tctgcgggcg attacccagt acatcggcga taacgccaaa
     3601 tctttccaga atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcggg
     3661 ctgttgtcgc tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc
     3721 aaagacttga tgcgcaccga tgccaacggt aaaggcgtta ttaatatcct cagcgccgag
     3781 aagctttatc agatgccgaa actgtacgcc gccagcctgc tgtggatgat ttcagagttg
     3841 tatgaacaat tgccggaagc gggcgatctg gagaagccaa aactggtgtt tttcttcgac
     3901 gaagcacatc tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg
     3961 ataaggctta ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat
     4021 attccggata atgtgctcgg ccagctcggt aatcgcgttc agcacgcttt gcgggctttt
     4081 acgccaaaag atcagaaagc ggtaaaagct gcggcgcaaa ccatgcgggc caatccggcg
     4141 tttgataccg aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc
     4201 gatgcaaaag gaagtccttc tgtggtggaa cgggcgatgg tgattgcgcc ttgttcgcgg
     4261 atgggaccgg tgacggaaga tgagcgcaat ggcttgatta accactcccc ggtgtatggc
     4321 aagtacgagg atgacgtgga ccgggaatcc gcctatgaga tgttgcaaaa aggcgtacag
     4381 gcgagtattg aacagcaaaa taatcctcca gcgaaaggga aagagatagc ggtagatgac
     4441 ggcattcttg gtggattgaa ggatattttg ttcggcacta ccggaccacg cggcgggaag
     4501 aaagatggtg tggtgcagac aatggccaaa agcgccgctc gccaggtgac gaatcagatt
     4561 gtacgcggga tgttggggag tttgctggga gggagaagaa ggtaagtcag gcgcgatctg
     4621 tccccctcac cctaaccctc tccccaaagg ggcgagggga ccgatcgaac tcgtatttga
     4681 ctttatcaac aatgtgaaag ggagctttcg ctcccttgct tggttacgat tttctcatta
     4741 acagccacag gctgattaag aagaagctgg cgcttggcaa cagtgcgccg ataatcggcg
     4801 ggatgccgta aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     4861 cgaagctgat accggtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     4921 tgaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     4981 acatattgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     5041 tgtgcaaacc gctgattgag agtgcatccg ggtccagcgc caccacgccc agtttgtctg
     5101 gcgtgaggtt ggttttccag gtgccgctca ccgtctgcga accggtgatc tgtttcggat
     5161 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     5221 tcgcagtagc ggcatagcgt acggattgca gacgacgatt ttcgttaaag gcataaatgc
     5281 tgatgccacc taactcttcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat
     5341 ctttcgccca taagccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     5401 ggtagttacg cgccatctgc tcgccctgcg gcgcgaccca ttcgccaatc gccatcgtca
     5461 gcaagaccag cggaatggcg gttttcatca ccgacagcgc cacctgcata cgggtaaaac
     5521 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     5581 ccccaagcag agccgccatc gggaagaaga tttgcacatc tttcggcacg ctcagcaagg
     5641 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc aactgatcga
     5701 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     5761 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     5821 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     5881 cgccaaagcc agataaatca ggttaacggt ccacatccac agcgtcgggt ccagcttacc
     5941 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     6001 ggctggcagc atcgacagta cgcgtccctg acgtgggtta accacgctca gcggtacgac
     6061 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     6121 agcacgatcg gtgtcagtgt tccacaatgt gcacatgtcc atctggtcgg tatcgttcgg
     6181 gtcgagcgcc accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg
     6241 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     6301 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     6361 attacctttt ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     6421 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     6481 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     6541 cggtcccgcc cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat
     6601 tgccgctttc accagaaccg ctttgctcag gccgcaggca tgcattaccg taatttcact
     6661 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     6721 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     6781 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     6841 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     6901 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     6961 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     7021 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     7081 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     7141 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     7201 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     7261 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgtcggg
     7321 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     7381 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     7441 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcaatg gaagcggtct
     7501 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     7561 tcgagacggc aaaagaaacg ctctacagtt tcgatcagct gaaaacgaac aagagcgaac
     7621 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     7681 gtgagcgcgc gatccagcac ggtctggcga ttgccgccgg gattaaagca gcaaaggatc
     7741 ttggcaatat gccgccgaat atctgtaacg ccgcttacct cgcttcacaa gcgcgccagc
     7801 tggctgacag ctacagcaag aatgttatca cccgcgttat cggcgaacag cagatgaaag
     7861 agctggggat gcattcctat ctggcggtcg gtcagggttc gcagaacgaa tcgctgatgt
     7921 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     7981 aaggtttaac cttcgactcc ggcggtatct cgatcaagcc ttcagaaggc atggatgaga
     8041 tgaagtacga tatgtgcggt gcggcagcgg tttacggtgt gatgcgtatg gtcgcggagc
     8101 tacaactgcc gattaacgtt atcggcgtgt tggcaggctg cgaaaacatg cctggcggac
     8161 gagcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga
     8221 atactgacgc tgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gagcgttttg
     8281 agccggaagc ggtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
     8341 atcacattac cggtctgatg gcgaaccata atccgctggc ccatgaactg attgccgcgt
     8401 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
     8461 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
     8521 caggttgctt cctgtcgcgc tttacccgta agtacaactg ggcgcacctg gatatcgccg
     8581 gaaccgcctg gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg
     8641 cacagttcct gttgaaccgc gctgggttta acggcgaaga gtaattgcgt caggcaaggc
     8701 tgttattgcc ggatgcggcg tgaacgcctt atccgaccta cacagcactg aactcgtagg
     8761 cctgataaga cgcgcaagcg tcgcatcagg caatgctgtt attgtcggat gcggcatgaa
     8821 cgccttatcc gacctacaca gcaccgaact cgtaggcctg ataagacaca acagcgtcgc
     8881 atcaggcaat gctgttattg tcggatgcgg cgtgagcgcc ttatccgacc tacacacagc
     8941 actgaactcg taggcctgat aagacacaac agcgtcgcat caggcgctgc ggtgtatacc
     9001 tgatgcgtat ttaaatccac cacaagaagc cccatttatg aaaaacgcga cgttctacct
     9061 tctggacaat gacaccaccg tcgatggctt aagcgccgtt gagcaactgg tgtgtgaaat
     9121 tgccgcagaa cgttggcgca gcggtaagcg cgtgctcatc gcctgtgaag atgaaaagca
     9181 ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca gaaagctttg ttccgcataa
     9241 tttagcggga gaaggaccgc gtggcggtgc gccggtggag atcgcctggc cgcaaaaacg
     9301 cagcagcagc ccgcgcgata tattgattag tctgcgaaca tgctttgcag attttgccac
     9361 cgctttcaca gaagtggtag acttcgttcc ttatgaagat tctctgaaac aactggcgcg
     9421 cgaacgctat aaagcctacc gcgtggctgg tttcaacctg aatacggcaa cctggaaata
     9481 atggaaaaga catataaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag
     9541 cagggctact ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat catgatcccg
     9601 ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct tccagcaaac catcatggat
     9661 accatgatcc gctatcagcg catgcagggc aaaaacaccc tgtggcaggt cggtactgac
     9721 cacgccggga tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga agaaggtaaa
     9781 acccgtcacg actacggccg cgaagctttc atcgacaaaa tctgggaatg gaaagcggaa
     9841 tccggcggca cgattacccg tcagatgcgc cgtctcggca actccgtcga ctgggagcgt
     9901 gaacgcttca cgatggacga aggcctgtcc aatgcggtga aagaagtttt cgttcgtctg
     9961 tataaagaag acctgattta ccgtggcaaa cgcctggtaa actgggaccc gaaactgcgc
    10021 accgctatct ctgacctgga agtggaaaac cgcgaatcga aaggttcgat gtggcacatc
    10081 cgctatccgc tggctgacgg tgcgaaaacc gcagacggta aagattacct ggtggttgcg
    10141 actacccgtc cggaaaccct gctgggcgat accggtgtag ccgttaaccc ggaagatccg
    10201 cgttacaaag acctgattgg caaatatgtc attctaccgc tggttaaccg tcgtattccg
    10261 atcgttggcg acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa gatcaccccg
    10321 gcgcacgact ttaacgacta tgaagtcggt aaacgtcacg ccctgccgat gatcaacatc
    10381 ctgacctttg acggcgatat ccgtgaaagc gcccaggtgt tcgataccaa aggtaacgaa
    10441 tctgacgttt attccagcga gatcccggca gaattccaga aactggagcg ttttgccgca
    10501 cgtaaagcag tcgttgctgc ggttgacgca ctcggcctgc tggaagaaat taaaccgcac
    10561 gacctgaccg ttccttacgg cgaccgtggc ggcgtagtta tcgaaccaat gctgaccgac
    10621 cagtggtacg tgcgtgccga tgtactggcg aaaccggcgg ttgaagcggt tgagaacggc
    10681 gacattcagt tcgtaccgaa gcagtacgaa aacatgtatt tctcctggat gcgcgatatt
    10741 caggactggt gtatctctcg tcagctgtgg tggggtcacc gtatcccggc atggtatgac
    10801 gaagcgggta atgtttatgt tggccgcaac gaagaagaag tgcgcaagga aaataacctc
    10861 ggtgccgacg ttgccctgcg tcaggacgaa gacgttctcg acacctggtt ctcttctgcg
    10921 ctgtggacct tctctaccct tggctggccg gaaaacaccg acgccctgcg tcagttccac
    10981 ccaaccagcg tgatggtatc cggcttcgac atcatcttct tctggattgc ccgcatgatc
    11041 atgatgacca tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc gttccacact
    11101 gtttacatga ccggtctgat tcgtgacgac gaaggccaga aaatgtccaa atccaagggt
    11161 aacgttatcg acccgctgga tatggttgac ggtatttcgc tgccagaact gctggaaaaa
    11221 cgtaccggca atatgatgca gccgcagctg gcggacaaaa tccgtaagcg caccgagaaa
    11281 cagttcccga acggcattga gccgcacggc accgatgccc tgcgcttcac cctggcggcg
    11341 ctggcgtcta ccggtcgtga catcaactgg gatatgaagc gcctggaagg ttaccgtaac
    11401 ttctgtaaca agctgtggaa cgccagccgc tttgtgctga tgaacacaga aggtcaggat
    11461 tgcggcttta acggcggcga aatgacactg tcgctggcgg accgctggat tctggcagag
    11521 ttcaaccaga ccatcaaagc gtaccgcgaa gcgctggaca gcttccgctt cgatatcgct
    11581 gcaggcattc tgtatgagtt cacctggaac cagttctgtg actggtatct ggagctgacc
    11641 aagccggtaa tgaacggtgg caccgaagcc gaactgcgcg gtactcgcca tacgctggtg
    11701 actgtactgg aaggtctgct gcgcctcgcg catccgatca ttccgttcat caccgaaacc
    11761 atctggcagc gtgtgaaagt actttgcggt atcactgccg acaccatcat gctgcagccg
    11821 ttcccgcagt acgatgcatc tcaggttgat gaagccgcac tggccgacac cgaatggctg
    11881 aaacaggcga tcgttgcggt acgtaacatc cgtgcagaaa tgaacatcgc gccgggcaaa
    11941 ccgctggagc tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac
    12001 cgtggcttcc tgcaaaccct ggcgcgtctg gaaagcatca ccgtgctgcc tgccgatgac
    12061 aaaggtccgg tttccgttac gaagatcatc gacggcgcag agctgctgat cccgatggct
    12121 ggcctcatca acaaagaaga tgagctggcg cgtctggcga aagaagtggc gaagatcgaa
    12181 ggcgaaatca gccgtatcga gaacaagctg gcgaatgaag gctttgtcgc ccgcgcaccg
    12241 gaagcggtca tcgcgaaaga gcgtgagaag ctggaaggct atgcggaagc gaaagcgaag
    12301 ctgattgaac agcaggctgt tatcgccgcg ctgtaatcaa caccgtgatg aaaacgaagg
    12361 ccggagaatg ctccggcctt ttttatctct tacagaaatg gtaaagaagg cataattccc
    12421 cgtgaaatac tggctaagaa gcctttatct ggttgttctt ctttattgat taaaggaagg
    12481 tcatccaaat cgcccagcaa atacgtattt tttgcctgcc agtcaatcat ccatattttc
    12541 agcagtggat aagccagacc gcccgtaatc ccggatatca ctaccaacgc acacatgcga
    12601 taaagcatac cgtggtaagt taaagttgag cgaaaacgaa tacgcccatc attcagtgac
    12661 aggttgctca taaaatgatt tcgcaacgac accgttaaat aactggtgct aacagcaatc
    12721 ccaaaataat agattaactg cgcgattatc attttacgtt gcatttccat aaattgctgt
    12781 aaattctcaa tatcatcatt tgcatacaca gaactatcat acgcatttaa tatttgatcg
    12841 aagataatat aaccagcaac agcgagaaaa ggtaataaag ctaaaaatgc gaggatggca
    12901 tattttatac aatacgcagt atctaatttc accttgaaac gatgtatacc gaaactggta
    12961 ttgctccaga gaaaactcat taccaggcta tataaagtac cattaaaaat accaatggaa
    13021 acaataccaa ctattgccat cagaaccaca gatataataa cactacttga actattggca
    13081 tgtagcatct ttgtcgagat aaagaaaaca gtccccatcc caatggccat taaaatcggc
    13141 aagaaaaagg ttactcacca gaaccctttc atagagcaat taaaactaaa tcttacgccg
    13201 ttgagactag tcatcaaggc ctgataacgc agtccttttg ctgccataaa tataagcaaa
    13261 accaacagtg acaaagtcaa aacacagcca ataagcggca tatctgctga cactgtcatt
    13321 aagattgcga aatagaaaaa aacaaaaaca agacaactaa caaaaacatt cccaccggta
    13381 atcccataag aaaatcgttg tccgttaacc tccatattag catagagata acgcttacat
    13441 ttcattaatg cccatggtaa atagatacct agcgttataa tcgttaacaa cacattcacc
    13501 acacaaataa gaaaatatct ctcacctgta ccatgaaaaa caaacgaatg ggacggaaca
    13561 tccatttcat taataacttg agccataaaa attccctgaa ttaaaaaatc cattgatata
    13621 agttatagat acagacagta aaatgttctg ccacgttaca atactcttat cagtaatagg
    13681 ctggcaaaaa ctttattgct ttataagatg cttgcgcacg taagaagaga gtggtattaa
    13741 acaaatgtat aattacttta tttttattgg gttaaactat gaataacatt gcgccgcaat
    13801 cacctgtaat gcgtcgcctg acgctgcagg ataatcctgc tattgcccgc gtcattcgtc
    13861 aggtatccgc cgaatacggt cttaccgctg ataaaggcta caccgtcgcc gatccgaatc
    13921 ttgacgagct gtatcaagtg tatagtcagc cgggtcatgc atattgggtg gttgagtacg
    13981 agggtgaagt ggtcggcggc ggtgggattg cgccattagc aggtagtgaa tcggacattt
    14041 gcgaactgca aaagatgtat tttcttccgg ctattcgcgg taaagggctg gcgaaaaaac
    14101 tggccttaat ggcgatggag caggcacgag agatgggctt caaacgctgc tatctggaaa
    14161 cgaccgcttt tttaaaggaa gccattgcgc tttatgagca tttgggcttt gagcatatcg
    14221 actatgcgct tggctgcacg ggccatgtcg attgtgaagt gcggatgcta cgcgaactct
    14281 aaaaaaatgc ccgctagtgt gtgctatcgg gcattttaat caattaatta gtggcgaacc
    14341 ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccgt cttcgccgtt gggatcttca
    14401 aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct ccgccagcgt catcagttgt
    14461 tcaacctggg catcgatcag atcggcattc aacgcgcact cgctgaggat gtcgcagcaa
    14521 atcacgatat caccatcttc aacttccagc tcttctggat cggtcacttc gtaaccgagt
    14581 ttaaacgctt caactgctgc tttttccagg gtttccagat cgtctgcgga aagatggtgt
    14641 tcgatggtat acagcgcgtc cggatcgctg ccatcttcca gtaattcttc aataatcaaa
    14701 cgtgtttctt cacgctgttc ttccagttgt tccgggtttg ccatggctca ttcctcttaa
    14761 tgtcctgccg atactcttat tgtcacacac cactgacatt gcctccacct ttgtgacaaa
    14821 gatttatgct ttagacttgc aaatgaataa tcatccatat aaattgaatt ttaattcatt
    14881 gaggtgttag ccacaggagg gatctatgtc cgggttttat cataagcatt tcctgaaatt
    14941 actcgatttc acgccagctg aactcaacag cctgctgcag ttagccgcga agctgaaagc
    15001 cgataagaaa agcggcaaag aagaagccaa actcactggt aaaaacatcg cgctcatctt
    15061 cgaaaaagac tcgacccgta cccgatgctc tttcgaagtt gccgcatatg accagggtgc
    15121 tcacgttact tatctcggcc caagcggcag tcagattggt cataaagagt cgattaaaga
    15181 cactgcccgc gtgctgggtc gcatgtatga cggtattcag tatcgcggct atggtcagga
    15241 gattgtcgaa acactggcgg aatacgctgg cgtgccggta tggaacggcc tgaccaatga
    15301 gtttcacccc acgcagttgc tggcggatct cctcaccatg caggagcatt tacccggtaa
    15361 agcattcaac gaaatgacgc tggtctatgc aggtgacgca cgtaacaaca tgggcaattc
    15421 gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt ctggtcgcgc cacaggcatg
    15481 ctggccggaa gctgcgctgg ttacggaatg ccgcgctctg gcacagcaaa atggcgggaa
    15541 tattacgctg acggaagatg tagctaaagg agttgaaggt gctgacttta tctataccga
    15601 tgtatgggtg tcgatgggtg aagcaaaaga gaaatgggct gaacgcattg cattgctgcg
    15661 tgattatcaa gtgaacagca agatgatgca gttgaccggt aacccggagg tcaaattcct
    15721 ccactgcctg cccgcgtttc atgacgacca aacgacgctt ggcaagaaaa tggcggaaga
    15781 gtttggccta catggcggaa tggaagtgac tgatgaggtc ttcgaatctg ccgccagcat
    15841 tgtatttgat caggcggaaa accgcatgca tactatcaaa gcggtgatgg tcgcga
//
LOCUS       DAZFRJ010000100        15824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000100 DAZFRJ010000000
VERSION     DAZFRJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.100"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(12..1244)
                     /gene="ycaQ"
                     /locus_tag="ACHKUN_002899"
     CDS             complement(12..1244)
                     /gene="ycaQ"
                     /locus_tag="ACHKUN_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO6661121.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(1281..2267)
                     /gene="lpxK"
                     /locus_tag="ACHKUN_002900"
     CDS             complement(1281..2267)
                     /gene="lpxK"
                     /locus_tag="ACHKUN_002900"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO6661122.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(2264..4012)
                     /gene="msbA"
                     /locus_tag="ACHKUN_002901"
     CDS             complement(2264..4012)
                     /gene="msbA"
                     /locus_tag="ACHKUN_002901"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO6661123.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(4049..6313)
                     /locus_tag="ACHKUN_002902"
     CDS             complement(4049..6313)
                     /locus_tag="ACHKUN_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO6661124.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(6520..6804)
                     /gene="ihfB"
                     /locus_tag="ACHKUN_002903"
     CDS             complement(6520..6804)
                     /gene="ihfB"
                     /locus_tag="ACHKUN_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO6661125.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(6964..8637)
                     /gene="rpsA"
                     /locus_tag="ACHKUN_002904"
     CDS             complement(6964..8637)
                     /gene="rpsA"
                     /locus_tag="ACHKUN_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO6661126.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(8748..9431)
                     /gene="cmk"
                     /locus_tag="ACHKUN_002905"
     CDS             complement(8748..9431)
                     /gene="cmk"
                     /locus_tag="ACHKUN_002905"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO6661127.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(9604..10368)
                     /gene="ycaL"
                     /locus_tag="ACHKUN_002906"
     CDS             complement(9604..10368)
                     /gene="ycaL"
                     /locus_tag="ACHKUN_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO6661128.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(10537..11820)
                     /gene="aroA"
                     /locus_tag="ACHKUN_002907"
     CDS             complement(10537..11820)
                     /gene="aroA"
                     /locus_tag="ACHKUN_002907"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO6661129.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(11891..12979)
                     /gene="serC"
                     /locus_tag="ACHKUN_002908"
     CDS             complement(11891..12979)
                     /gene="serC"
                     /locus_tag="ACHKUN_002908"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO6661130.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(13178..13870)
                     /locus_tag="ACHKUN_002909"
     CDS             complement(13178..13870)
                     /locus_tag="ACHKUN_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO6661131.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            14000..15760
                     /gene="ycaO"
                     /locus_tag="ACHKUN_002910"
     CDS             14000..15760
                     /gene="ycaO"
                     /locus_tag="ACHKUN_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO6661132.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         4017 a         4214 c         3743 g         3850 t
ORIGIN      
        1 atcataatca tttatgcgac ggggtctatt tcccagccag tgcggcaatc agtaaagaga
       61 ccttgcgggc agcgtcctaa tgtcacccgc gttgcctgct gccagctcgc gaaatcagta
      121 atcgcctgac gtaacccttt ttgcagcatt gtcgttggtt taatgccttc ctgtaaccac
      181 agagagataa cttcaaggat gcctgtctgg cgatgcattt tggcatccat tcgcccaact
      241 aattgcccac gatgtaataa tggcagaaca aaatagccat actggcgttt cggcgctggg
      301 gtatagcact ccagccggta gctaaaatca aaaagctgct ctgcgcgttt gcgatcccag
      361 acaacaggat caaaaggcga aagtaccgcg ctgtgcgttg cagtgagctt tccggccagc
      421 gcacgctcga gtagcggcag caaatcagca tgcagccaaa gattgcccaa tttttcaaca
      481 tgcacagcaa tgatttgctg ctgttcagcc ctcgcttcgc gccacgccgc cagtgccgga
      541 cgtttcagcc gatagtaatc tgccagccac tgttcgcgga atattcccag gctacgcgca
      601 ctgttatcca gcatgatgat ttctgcttct gtttgcgaaa cgagatcgcg ctcatcatcc
      661 cagtcaggca tgacacggtg ggttaaatca taaacgcgct ggaagttgcg ccgttcaatc
      721 accatcacct ttccggcagt aaataaacct tccagatgcc gtttatgcgg cttccattcc
      781 caccagccgc ttgcaccttt acgaggatgc tcaaaatcgg ctgaacgtac cggcccctta
      841 tcatgaatat gctgaattaa ctgtgcaatt tccgcctcat gttcctgcat ccaggcgtct
      901 ttgtatttcc agcccatttt ttcaggtgcc agcatgcggt ggcgaataag acgaaagtcg
      961 ctacgcggca taaagcaggc ttcatgcgcc cagtattcca ttaattcgcc acgcgccaga
     1021 gactcatcca gccactgggc aggataattt cccagacgac tgaaaagcac cagatatgga
     1081 ctacgggcaa caatattgat ggtatcgatt tgcagcaagg acatgcggga gatcgttgcc
     1141 ggaatatcct ccaacgacgc ttgacggcgg ggtttgttta acaggccttg tgcggcaagg
     1201 tgaagattac gcgcatcagc aagggagagg tgcggcagcg acattcatga ctccatcaat
     1261 cgaacgctgc cgcggcgtaa ctagttgcca gaagccagcg aggttagttg cgtaagcagt
     1321 ttcgctggtt catcacctga aagctgtgcg tctacaggca aataccacca attttcttct
     1381 gcaaaggccc ggcatttcac cgcatctttt tcagtcatta ccagcgtttg cccggcgctt
     1441 accaacgcac tgacatccgc atggttcaaa gactgatgat cggccagcgg tacacatttt
     1501 tccggttgta cgccacacat cttcagcgtg gcaaaaaagc gcggcggatg cccaatcccc
     1561 gccatcgcca ctacatgttc aagctgagca acgtcacaac gcgtaccggt acgtaaattc
     1621 accgcctgac ccggcagcag atgcatgggg atttcaccgc tgcgagggac accgccgttg
     1681 acgattaccg catcaaccga ctttaagcgc cccgctcgct cacgcattgg ccccgccggc
     1741 aaccaccagc cattgccaaa gcgacgcaca ccatcaataa cgacaatttc cacatcacgc
     1801 gccagacggt aatgctgtaa accgtcgtcg gttacgatga tctgcacatc agggtgttgc
     1861 gccagaatgg cttttaccgc atcagaacga acgggagaaa ccgcaacagg cgcatcagtg
     1921 cgttgataaa tcaacacagg ttcatcaccc gcctgtgctg ttgtggtatc tgccgacaat
     1981 aacagcggat aagattcagc cttaccacca tatccccgcg atacgacccc cacgcgaata
     2041 ccgcgctgtt gcaactgttc caccagccag acaacgaccg gggtttttcc gttgccgcct
     2101 gcggtgagat taccaaccac gacaaccggt acgggggcac gccaggcgcg cttcagtttt
     2161 agtttatagc aaagacggat cgcgccactt accaggccat acaaccagga gagtggcagc
     2221 aatagccgcc acaaagggga ttcaccagac cagatttttt cgatcattgg ccaaactgca
     2281 ttttgtgaag ttgcgcgtaa acgccgcggt gctcaagcaa atcgttatgc gtaccgcgtt
     2341 ccacaatgac accatcctcg acgaccacga tttcgtctgc cttttcaatg gtagacaagc
     2401 ggtgggcaat caccagagag gtacggtttt tctgcaactc atccagtgcc gcctgaatcg
     2461 cacgttcgga ttcggtatcc agagccgagg tagcttcatc cagaatcaga atcgggctat
     2521 cacgcagcaa ggctcgagcg atagcaatac gctgacgctg accgccagag agcagcacgc
     2581 cgttttcacc aatcactgta tcgagaccgt tatccatctt attgatgaag tccatggcgt
     2641 aggccatacg cgccgcttct tcaatttgct cacggctgta ctgttcagtc cgtgcgtaag
     2701 caatgttgtt agcaaccgta tcgttaaaca gatggacatt ctgcgacacc agagcaacct
     2761 ggttacgtaa cgacgccagg gtatactcgc gcagatcgtg accatccatc aggatttcgc
     2821 cttcatcaat atcgtaaaaa cgagtgatca ggctggcgat ggttgattta cctgaaccag
     2881 agcgtccaac cagagcaacc gtcttccctg ccggaatttt caggttgatg ttacgcaatg
     2941 caggtacgtc acgtcccgga taagtaaagg tgacattgcg gaattccacg tcgccagtcg
     3001 cacgctcgat cacgcgctta ccttcgtctt tctcctgctc actgtccaga atggtaaaca
     3061 gcgtctgaca agccgccata ccgcgctgga actgggcgtt aacgttggtc agtgatttca
     3121 gcggacgcat cagtgcaatc attgaagaga aaacaacggt aatcgtaccg gcagtcaggc
     3181 tatccatgac acttgggaag ctcgccgcat acagaacaaa cgccagcgcc aaagaggcga
     3241 tcagctgaat gatcggatca gagatggaag aggctgaaac cattttcatc ccctgaagac
     3301 gcattcggtt gctgacttta tcaaagcgtt tcgtttccac ttcctgacca ccgaaaatca
     3361 atacttcttt gtggcccttc agcatttgtt cggcgctggt ggtcacctgc cccatggtgt
     3421 tctgcatgtt tttactgatg ttgcgaaaac gcttcgatac tacgcgaatc gcaatcgaaa
     3481 caatcggtgc cagcacaatc aaaatgatcg acagttgcca actgtaatag aacatcatga
     3541 tgaacaggcc gatgattgac gcaccttcac gcacaaccgt tatcagtgcg ccggaagaag
     3601 aagaagcaac ctgttcggaa tcgtaggtga tacgtgacaa cagcgtcccc gttgactgtt
     3661 tgtcaaaaaa tgaaaccggc attcccatca tgtgaccaaa caggcggcga cgcatggtca
     3721 ttaccacctt tcctgatacc caggagatac agtagctgga gacatagctg gtgataccac
     3781 gtaaaatcat cagcccgatc accaccagcg gcatccacac cagcacggag cgatctgttt
     3841 taccaaagcc atcatcaaga agtggcttaa ggagcgataa catgaaggta tcgctggctg
     3901 cgttgaggat taacgctacg cccgccacga tcagacccgc tttaaaaggc gcaatggttg
     3961 gccacagtcg gcggaacgtc tgccacgtag agagatcttt gtcgttatgc attcaaaaaa
     4021 ccagcatttg ttgaaatagc cgcatattct acccgttatc cactggcacg ccaaaccact
     4081 gatgatacca acgaggtaaa atttgctctc tgaggctgct aatccgccag ccttgcgctg
     4141 aaaaattgac cgtcacttga ccctgatgtg gagtatcaag ccattgatat ccttgctgtt
     4201 gatagcgatg cttaacttta ttagagggca atcgccatgc gttatagcgc gatgccgatg
     4261 cgagtgccac ttttccattc actcgctgaa ttaatggcaa cgatgatgag gtattactgc
     4321 catggtgagg tacctgaagc aatgttgtct gcacttgctg ccagtaacgg cttagcatct
     4381 tttgttcagc ggggacttca atatcaccgg ttagaagaat gctattcgtc ccgtcatcaa
     4441 ccttaaccac acaggaatgg ttatttcctt tatcgttgct agcttgtaaa ggccagtgca
     4501 cgctgaaacg caatccttgc cattgccacg cttcgccacg cacacagggc tgatgatgtt
     4561 cccagtttaa cggacttctg atccataaca tcggccatgt gtgcaatatt gagtccagcc
     4621 ctccccggtg atccagatgt tcatgactca gaataacgcc ttccggttca agattatgcc
     4681 agtggagcca ggggataatc agttgttgcc cactatcccc ttcgggccag gccagtcctg
     4741 tgtcatagag aatcgctttg ccgtttctgg ctatcaccat tgccagccct tgcccgacat
     4801 caagcatgta cacctgccac tcgtcaggtc gaggtttttg ccacagcggc cagcacatca
     4861 gcaagcctac agccacacac attgctggca acgttcgcca ggcgtttaat cgccatacca
     4921 ccagtaagaa ccatggggaa aatgatagcc attgccaacg ttcagcaatg ttgatccacc
     4981 cttctggcaa actctttaac ccccagaaaa gtaaagccaa agaccggtcg gcaagaaacc
     5041 ataacccttg ctccaggatt aacggcccgc ttaaatgcac aaccatcgcg gcgaggatca
     5101 acggaaccgt gataaatgtc accaagggaa ttgctaatag atttgcaata aacgaggtca
     5161 gactaatgcc atgaaatatg acgatttgca caggcataag caggagtgtg attcccagtt
     5221 gcaaatgaat gagggaaaca actgcacgca ataccggcgg cagttgccac tcaggacagg
     5281 gaaaccactg gtaccaaaaa atcagtgccg cgaccgcagc ggcagagagc cataaacttt
     5341 gcgagagaat ggcaacagga tccatcagca aaattgccgc cagacaacat atccatacat
     5401 cccagccact ccactgtcgc ccacttaact taagcattcc ccacgtagca agcgccacca
     5461 cggtacgcaa tgcaggaggt tgcattcccg tcaaccaggc ataaaaagca gcacagcaga
     5521 ttccaccaat taatggcatt tgccagtgga tccagcgccc aggcagataa acttgcccac
     5581 cgcgaatgag tccggcagcc agcaacgccg caaaagcgat gtgcaatccc gatatcgcca
     5641 ttaaatgcgc cgttccagta tcacgcatga tatttttgat ttctttgggg acggataacc
     5701 gttcccccat acctaaacca agaataaccg cattccacat atagggttgc agcgttgttt
     5761 gtagtgacgc cagatactgt gcacgcaggc tacaattcgg ttcaatgaca cttgcctgca
     5821 gaaagcggcc ggtgagcggc tgatgctggg caatggcata acgctggcta tcaaagccgc
     5881 catcattaag ttggccatga actgcacgaa ctttgagttt cattgaccat tgttgtccag
     5941 cacaaaccgc ttgcggaaga tattcgccat acagcacaag gcctggcgca gggaatatac
     6001 gtcgaccttg tagatgagta atttgaccgt aatgagtggt catgccgtca gttgcagtga
     6061 tctcaacaat tgcatcctgc gtcgcgccag ttaaggtttc tcctgcccac aaaatttgct
     6121 ttgctgataa tatgccccaa acgaaaaaca gcaacgtcag cgcgatataa cggacggttt
     6181 taacagggat aaatgccagt acgcaggcga agagagtcag aaacgcaagg gttaatgtcc
     6241 caggcaattg gggcaaaatc agcaacggaa aaattccgca aattatgcat acgccgactg
     6301 tcgttatttt catgcacccc tccctgttta tggaaagtgt gcaactttgt tgagcggttg
     6361 tctggtggga attttgcgtt ccccgaggca cattcagaag aatttaacga aagggtgaaa
     6421 actgctcaaa actctgcgag gcagcttcca gttcctgatt agagaaatga aaaaagcacc
     6481 cgacaggtgc ttttctcgcg ttcaagtttg agtaaaaact taaccgtaaa tattggcgcg
     6541 atcgcgcagt tctttaccag gtttaaagtg aggaacgtat tttccttcca gttctacttt
     6601 atcgccagtc ttcggattac gtccggtacg tggtgcgcgg tagtgcaaag agaaactgcc
     6661 gaaaccgcgg atttcaatac gctcgccctg cgcaagagtc gaggccatat gctccagcat
     6721 ctcttttact gcatcttcaa ccgtcttggc gggaatgtgc gattgctggg tggcaagtct
     6781 ttctatcaat tctgacttgg tcatgattcc tccggttcct taaaggcaaa ttaagcggct
     6841 gctgttgctg cattgattaa gggcggccgt agccgccctt agtgcttgat tacaggacga
     6901 aacctgcaat ctgtcaagta aactcaacaa acttcggaat aaaaatcccg aagagtcaga
     6961 gaattactcg cctttagctg ctttgaaagc ttcagccatt gcgttgttgg agaagtttgc
     7021 atcttcctgt ttgttaacag ttgcgattgc atctttctcg tcagcttcgt ctttcgcacg
     7081 aacagacagg ctgattgcgc ggtttttacg atcaacgccg gtgaatttag cttcaacttc
     7141 gtcgccaacg ctcagaacca gggtagcgtc ttcaacgcgg tcacgggatg cttcagaagc
     7201 acgcaggtaa ccttcaacgc cgtcagccag ttctacggtt gcgcctttag cgtcaactgc
     7261 agttacttta ccggttacga tagcgccttt cttgttcaga gcaacccagt tgttgaacgg
     7321 atcttctgcg agctgtttaa cgcccaggga gatacgttca cgttctgcgt caacctgcag
     7381 aacaactgca gcgatttcgt cgcctttttt gtattcacga actgcttctt cgcctgcaac
     7441 gttccaggag atgtcagaca ggtgaaccag gccgtcgatg ccgccgtcca ggccgatgaa
     7501 gataccgaag tcagtgatag acttgatttt accttcaaca cggtcgccct tgttgtgggt
     7561 ttccgcgaac tgctgccacg ggttagcttt gcactgtttc agacccaggg agatacgacg
     7621 acgttcttcg tcgatatcca gaaccataac ttccactaca tcgccaacgt taacaacttt
     7681 ggacgggtgg atgtttttgt tggtccaatc catttcggaa acgtgtacca ggccttcaac
     7741 gccttcttcg atttcaacga agcagccgta gtcggtcagg ttggtcacgc gaccagtcag
     7801 tttggtacct tccggataac gtttagcgat agctacccac ggatcttcgc ccaactgttt
     7861 caggcccagg gatacacggg tacgttcgcg gtcgaacttc agcactttaa cagtgatttc
     7921 gtcgcccacg ttgacgattt cgctcggatg cttaacgcgt ttccaggcca tgtcagtgat
     7981 gtgcagcagg ccgtcaacgc cgcccagatc aacgaatgca ccgtagtcag tgaggttctt
     8041 aacgatacct ttaacttcca tgccttcctg caggttttcc agcagctgat cgcgctctgc
     8101 gctgttttcg gattcgataa cggcacgacg agaaacaaca acgttgttgc gcttctgatc
     8161 cagcttgatt actttaaatt caagctcttt gccttccagg tgcagagtgt cacgcaccgg
     8221 acgaacgtct accagagaac ctggcaggaa cgcacgaata ccgttcagct caacagtgaa
     8281 gccgccctta actttgccgt tgataacacc ggtaacagtt tcagcatctt cgtaagcttt
     8341 ttccagcgtg atccaggctt cgtgacgttt agctttctca cgggacagca gagtttcacc
     8401 gaagccgtct tctactgcgt ccagagcaac gtcaacttca tcacctacct ggatttccag
     8461 ctcgccctgg gcgtttttga actgctcagc cgggatggcg gactcagatt tcagaccagc
     8521 gtcaaccagt actacgtctt tgtcgatagc aacaacaacg ccacgaacga tagaacccgg
     8581 gcgggtttcg atttctttta aggactcttc aaagagttga gcaaaagatt cagtcatgtt
     8641 taatcttcag gtttatattt aacgtccacc tggctccatg ccggatgggg ttgtttaaca
     8701 tacccgctgt cattccattg caacgggggt actgcaaatt cggtcgctta tgcgagagcc
     8761 aatttctggc gcgcgtattg tagcgctttt tcaatcactt gctcaatgct taaggtggtg
     8821 gaatccaaca ctaaagcatc ggctgccgga accagtggcg ctaccgctcg gttacgatcg
     8881 cggtcgtcgc gttctttgat ctcggccaaa aggcgctcaa agttaacact aaagcccttc
     8941 tcctgcaact gtagcatgcg gcgatgcgca cgttcttccg aggaggcgtc aaggaaaatt
     9001 ttcactggcg catcagggaa taccaccgtt cccatgtcgc ggccatcggc aatcaggccg
     9061 ggtaattcgc gaaacgcgcg ttggcgacgc aataatgctt cacgaacgcg tgggaatgcc
     9121 gcgacttgtg aagctgcatt cgccacttcc tgagtacgaa tttcgccgct gacatcttcc
     9181 ccttcgagga tcacttccag attgccattg gttgacacaa aacgtacatc cagatgggat
     9241 gccagcggta ccagcgcatc ttctgacgca acatcaacat ggtgatgtaa tgccgccaat
     9301 gccagtacgc gataaattgc acccgaatcc agcagatgcc attgcaatgc ttccgccata
     9361 gccttacaca aggtgccttt ccctgcaccg cttgggccat caatggtaat aaccggggca
     9421 attgccgtca tctttatctc cttaaaacag gcgtaccgtt aacataaccg cgcgcattat
     9481 acgcgccgac gccacaaatc gttaacgttg tgtgcaaaaa ccagttttgt ctgtattcag
     9541 atggggattt attgcgcaat tatagcgggc tggcagaaga ccagcccgaa agatgacaat
     9601 gatttactta ccagaggcga tacgatcacg gatgtgttgc gcacgctctg ttgatggtgg
     9661 gtgagagtca aacatggact gggtgcgacc accatccaga ctagccagtt tttcaaagct
     9721 gccaaccagc ccctgggtgc tgatgccacg tttcttcaac agatcaaagg agaaatcatc
     9781 tgcttcggac tctttatcac gggagtattt agcattaata gcgccttctg cgatatcacc
     9841 caattgtgag cgggaaagct gggaagccac accgctggta gctgaaatgg catcgcgtgc
     9901 ggcaacgatc gcataagaag ctttcatttc agccagcgag tgacccaacg cgacgtggcc
     9961 cagttcatgg cccagaacac cttcaatttc gttgtcattc atcatgtcca tcaggccact
    10021 gtagacacgg acacagccgt tcgccatcgc ccatgcgttg acgtcgctgg tcatataaac
    10081 cttatagttg accggcgtgc cattaatatt gttacccaat gctttggcga tttttgccag
    10141 acgtttaccg tacttgctgg atttacttgc gacttgattg ccgctgtcca tttcggcaca
    10201 gccctgattg gcaatcgctt ttgcatcggc atcgcttaat gttgctgctt tataggcatt
    10261 taaaccggag ctgatagcca tattggtatc aataccgtgg gtattttgac accctgtcag
    10321 taatgctgca gaggtcgcaa tcgccagcag taatttagta ttcttcatgt gaatcatccg
    10381 ttgattaatc atatataaat attatgtatc aacaacttac attgacaagc gacgctacca
    10441 ttaatcaaaa tgactcacaa ggtccgaaaa aaaacgccga caatgtgccg acgttttgat
    10501 aaagaaagat ttggctattt attgcccgtt gttcattcag gctgcctggc taatccgcgc
    10561 caactgctcg aaataatccg gaaatgtttt ggccgtacat ttgggatcaa gaatcgtcac
    10621 tggtgtatct gacaacgcca ccagcgagaa acacatcgcc atccggtgat cattgtacgt
    10681 cgcgatctcg gcaaagttca gtttttccgg tggagtgata cgaatgtaat cgtgcccctc
    10741 ttctacttcc gcaccgactt tacgcagttc tgttgccatc gcaaacaggc gatcggtttc
    10801 tttaacacgc cagttataga tattgcgcag cgtggtggtg ccttttgcaa ataacgccgc
    10861 cgtggcaatg gtcatcgccg catcgggaat atggttcata tccatatcaa tagcgttcag
    10921 ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa atggtcgcgc ccattttttc
    10981 cagcacatca gcaaagcgaa tatcaccctg catactgtta cgtccaatac cggtcacttt
    11041 tacagtgccg cctttgattg ctgctgctgc cagaaagtaa gaagccgaag atgcatcgcc
    11101 ttcgaccaaa taagtacccg gagactgata agactgcccg ccttttacga caaattgttg
    11161 atagtgctga ttttcaattt caacaccaaa cgtcttcatc agattgagtg tgatgtcgat
    11221 ataaggttta gaaaccagat cgcctttaat acgaatcacc gtatcttccg gcgcaagagg
    11281 cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg gagccatcaa cgtcaacgtt
    11341 gccgccagta aagccgccct gtaaacgcaa cggcggataa ttttcttgtt ccaggtaagt
    11401 gatcttcgcc ccgcccaggc gcagcgcatc caccagatga ccaatcgggc gttctttcat
    11461 acgcggctca ccggtcagca caatatcatt gctacccaga caaagagctg ccgccagcgg
    11521 acgcattgcc gttccggcgt taccgaggaa caactccagg gcaccttctg cgtgtaatgg
    11581 accgccgtta ccgataattt cgcaacgcgt acgatcggct gaaagcgtat agcttacccc
    11641 taacgctgtt aatgcattca gcatatggcg cacgtcatcg ctatccagca gattggttaa
    11701 tactgttttg ccgtgtgcta atgccgccag caataaagcg cggttagaaa cgctcttgga
    11761 accgggcaga ttaatagtgc catcgacacg agcgatgggt tgtaacgtca gggattccat
    11821 gaactcaact ctctacaaca gaaataaaaa ccccacaggc tggctgtggg gattaagcaa
    11881 aatttcggca ttaaccgtga cggcgttcga actcaaccat gaagtctgtc agcgctttaa
    11941 cgccttccag cggcatggcg ttataaatag aagcgcgcat tccgccgacc acacggtgac
    12001 ctttcagtgc atgaaggcca gcagcaaaag actcttcaag gaacaatttg tcaagcgcac
    12061 tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt agctttcgcc acgtcattgc
    12121 ggtagaaatc gctgttatca atcaccccat atagcagttc tgctttttgc tgattgattt
    12181 tatccatttc agctacaccg ccgttcgctt tcagccattt aaagaccaga ccagatagat
    12241 accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt atcgttgagg atggaataat
    12301 ccagaatcga cggacacgcg atattcgctt tgcccagcaa atcttcacga acgatgacga
    12361 ttgtcaggcc agccgggccg atatttttct gcgcgccagc gtaaatcacg ccataacggc
    12421 tgacgtcaat cggacgggaa agaatggttg aagagaagtc ggcggcgacc accacatctt
    12481 tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat ggtttcattc gggcagtaat
    12541 gcatataagc agcattatca gagagttgcc attcacgcat tggcttaacc gcgcgcagac
    12601 catcaacagt cactttggcg tcaaagacat taggcgtgca gtattttttc gcttctttaa
    12661 tggcacttgc cgcccagtaa ccggcatcaa cataatctgc ggtggtttta tcaccgagaa
    12721 tattcagcgg taccgcagca aactgaccgc gaccaccgcc atggcagaat aataccttgt
    12781 agttggaggg gacattaaga agatcgcgaa aatccttctc ggcttcctct gcaacctgaa
    12841 tgaactcttt gccacggtga ctcacttcca tcaccgacgt accaagaccg ttccagtcgc
    12901 gcagttcctg ttgagcctgt ttaagcacct ctgccggtag cattgccgga ccagaactaa
    12961 aattgaagat ttgagccatt tcccctcacc acgttgcgtt gctattaacc cgctgtcaat
    13021 cgattgcgac cacaggtaat acattattcc tgtggctatc ggttttatca ttcagtgaca
    13081 cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg cttgcatcac acaaaagaat
    13141 ctctcggatt attagcaaaa agccgatgtt tttactgtca ggtcacccgt tttgcccgac
    13201 ttgccttcgt ccattctgga tttgcacagc gcggacataa ttgtttttcc cccgctttca
    13261 tatgaatgat ttcactacaa cgaatacagg catagtccgc cgactccggc accactttgt
    13321 aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg tttcaccttg tcatcttcaa
    13381 agaaatagac actgatttgc ccgttggttt cgagaatcgc cagacgtacc tgccccagct
    13441 gctccacgcc acgcaatcgt agctccataa agaactcaaa ttccgtcatg ttggagttat
    13501 tgagtttcga ccaggccagc tcgccatctt caataataac aaccggctta ccttccagta
    13561 gatcttccag tttttcactg tgcgccatca accacattac caggcggtat aacaacgcca
    13621 gagtgataaa gacgataagt accggcacca tcggcacatc atcataaaac gccacatctc
    13681 ccgccgctga acccagcgtg agaatgatta acacttcaaa cagcgacatc tgtcgcacac
    13741 cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac aaaggtataa agactacgta
    13801 gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa tgccatacgg tggagatcga
    13861 acgccttcat ttttatgctc ttatcgtggt ttatccgcta aaagcatagt caacagttca
    13921 tcggcatggc ggcggtgata gcgcctttga gataaaaccc gtattattgc gcgctttccg
    13981 tacgactaaa gtgattttca tgacgcaaac atttatcccc ggcaaagatg ccgctctgga
    14041 agattccatc gctcgcttcc agcaaaaact ttcagacctc ggctttcaga ttgaagaggc
    14101 ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat attcgcgaca aagagtgcgc
    14161 actgtgtttt accaacggaa aaggcgcaac caaaaaagcg gcgctggctt ctgcacttgg
    14221 tgaatatttc gagcgtctct caaccaacta cttttttgct gatttctggc tgggcgaaac
    14281 catcgccaac ggtccgttcg tgcattatcc caacgaaaaa tggttcccac tgaccgaaaa
    14341 tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc gcgttttatg atccggagaa
    14401 tgaactgacc ggcagcatgc tgattgacct acaatccggt aacgaagatc gtggtatttg
    14461 cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt tatattccga tgaatatcat
    14521 tggtaacctg tacgtctcca acggtatgtc cgcaggtaat acccgcaacg aagcacgcgt
    14581 tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac cgcattattg ctgaaagcat
    14641 cagcctgcca gagatcccgg cagacgtgct ggcgcgttac ccagcagtag ttgaagccat
    14701 cgaaacgctg gaagcggagg gtttcccaat cttcgcttat gacggttcgc ttggcggcca
    14761 gtatccggtg atttgcgtgg tactgttcaa tcctgctaac ggtacctgct ttgcctcttt
    14821 cggtgcgcat cctgattttg gcgtagcact ggaacgtacc gtgaccgagc tgctgcaagg
    14881 tcgtggcctg aaagatttgg atgtgtttac tccgccaacc ttcgatgatg aagaagtcgc
    14941 tgaacatacc aacctcgaaa cgcactttat cgattccagc ggtttaatct cctgggacct
    15001 gttcaagcag gatgccgatt atccgtttgt ggactggaat ttctccggca ccacggaaga
    15061 agagtttgct acgctgatgg ctatcttcaa caaagaagat aaagaagttt atattgccga
    15121 ttacgagcat ctgggcgttt atgcttgccg tattatcgtg cctggcatgt ccgatattta
    15181 tccggctgaa gatctgtggc tcgcgaataa cagtatgggc agccatttac gtgaaacgat
    15241 tctttcgcta ccaggcagcg agtgggaaaa agaagattac ctgaacctca tcgagcaact
    15301 ggatgaagaa ggttttgatg actttacccg cgtgcgtgag ctgttgggtc tggcgaccgg
    15361 gtcggataac ggttggtaca ccctgcgtat cggtgaatta aaagccatgc tggcgctggc
    15421 tggtggcgat ctggaacagg ctctggtctg gaccgaatgg acgatggagt ttaactcatc
    15481 agtatttagt ccggaacgcg ccaactatta tcgctgcctg caaacgttgt tattactggc
    15541 acaggaagaa gatcgccagc cgctgcaata tctgaatgcg tttgttcgca tgtacggcgc
    15601 agatgccgta gaagccgcca gtgcggcaat gagcggcgaa gcggcgtttt acggcctgca
    15661 accggtagat agcgatctgc acgcgtttgc tgcacatcag tcgttgttga aggcctacga
    15721 aaagctgcag cgcgccaaag cagcattctg ggcaaaataa aatcaaatag cctacgcaat
    15781 gtaggcttaa tgattagtct gagttatatt acggggcgtt tttt
//
LOCUS       DAZFRJ010000101        15758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000101 DAZFRJ010000000
VERSION     DAZFRJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.101"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            276..680
                     /gene="sdhC"
                     /locus_tag="ACHKUN_002911"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             276..680
                     /gene="sdhC"
                     /locus_tag="ACHKUN_002911"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6661133.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            674..1021
                     /gene="sdhD"
                     /locus_tag="ACHKUN_002912"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             674..1021
                     /gene="sdhD"
                     /locus_tag="ACHKUN_002912"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO6661134.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1021..2787
                     /gene="sdhA"
                     /locus_tag="ACHKUN_002913"
     CDS             1021..2787
                     /gene="sdhA"
                     /locus_tag="ACHKUN_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO6661135.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2803..3519
                     /gene="sdhB"
                     /locus_tag="ACHKUN_002914"
     CDS             2803..3519
                     /gene="sdhB"
                     /locus_tag="ACHKUN_002914"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO6661136.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3820..6621
                     /gene="sucA"
                     /locus_tag="ACHKUN_002915"
     CDS             3820..6621
                     /gene="sucA"
                     /locus_tag="ACHKUN_002915"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6661137.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6636..7853
                     /gene="odhB"
                     /locus_tag="ACHKUN_002916"
     CDS             6636..7853
                     /gene="odhB"
                     /locus_tag="ACHKUN_002916"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6661138.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7947..9113
                     /gene="sucC"
                     /locus_tag="ACHKUN_002917"
     CDS             7947..9113
                     /gene="sucC"
                     /locus_tag="ACHKUN_002917"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6661139.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9113..9982
                     /gene="sucD"
                     /locus_tag="ACHKUN_002918"
     CDS             9113..9982
                     /gene="sucD"
                     /locus_tag="ACHKUN_002918"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6661140.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10086..10808)
                     /gene="mngR"
                     /locus_tag="ACHKUN_002919"
     CDS             complement(10086..10808)
                     /gene="mngR"
                     /locus_tag="ACHKUN_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO6661141.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10977..12893
                     /gene="mngA"
                     /locus_tag="ACHKUN_002920"
     CDS             10977..12893
                     /gene="mngA"
                     /locus_tag="ACHKUN_002920"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO6661142.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            12911..15544
                     /gene="mngB"
                     /locus_tag="ACHKUN_002921"
     CDS             12911..15544
                     /gene="mngB"
                     /locus_tag="ACHKUN_002921"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO6661143.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            15569..>15758
                     /locus_tag="ACHKUN_002922"
     CDS             15569..>15758
                     /locus_tag="ACHKUN_002922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661144.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIF"
BASE COUNT         3766 a         3965 c         4348 g         3679 t
ORIGIN      
        1 aggataaaac ccgacaaact atatgtaggt taattgtaat gattttgtga acagcctata
       61 ctgccgccag gtctccggaa caccctgcaa tcccgagcca cccagcgttg taacgtgtcg
      121 ttttcgcatc tggaagcagt gttttgcatg acgcgcagtt atagaaagga cgctgtctga
      181 cccgcaagca gaccggagga aggaaatccc gacgtctcca ggtaacagaa agttaacctc
      241 tgtgcccgta gtccccaggg aataataaga acagcatgtg ggcgttattc atgataagaa
      301 atgtgaaaaa acaaagacct gttaatctgg acctacagac catccggttc cccatcacgg
      361 cgatagcgtc cattctccat cgcgtttccg gtgtgatcac ctttattgca gtgggtatcc
      421 tgctgtggct tctgggtacc agcctctctt cccctgaagg tttcgagcaa gcttccgcga
      481 ttatgggcag cttcttcgtc aaatttatca tgtggggcat ccttaccgct ctggcgtatc
      541 acgtcgtcgt aggtattcgc cacatgatga tggattttgg ctatctggaa gaaacattcg
      601 aagcgggtaa acgctccgcc aaaatctcct ttgttattac tgtcgtgctt tcacttctcg
      661 caggagtcct cgtatggtaa gcaacgcctc cgcattagga cgcaatggcg tacatgattt
      721 catcctcgtt cgcgctaccg ctatcgtcct gacgctctac atcatttata tggtcggttt
      781 tttcgccacc agtggcgaac tgacatatga agtctggatc ggtttcttcg cctctgcgtt
      841 caccaaagtg ttcaccctgc tggcgctgtt ttctatcttg attcatgcct ggatcggcat
      901 gtggcaggtg ttgaccgact acgttaaacc gctggcattg cgcctgatgc tgcaactggt
      961 gattgtcgtt gcactggtgg tttacgtgat ttatggattc gttgtggtgt ggggtgtgtg
     1021 atgaaattgc cagtcagaga atttgatgca gttgtgattg gtgccggtgg cgcaggtatg
     1081 cgcgcggcgc tgcaaatttc ccagagcggc cagacctgtg cgctgctctc taaagtcttc
     1141 ccgacccgtt cccataccgt ttctgcgcaa ggcggcatta ccgttgcgct gggtaatacc
     1201 catgaagata actgggaatg gcatatgtac gacaccgtaa aagggtcgga ctatatcggt
     1261 gaccaggacg cgattgaata tatgtgtaaa accgggccgg aagcgattct ggaactcgaa
     1321 cacatgggcc tgccgttctc gcgtcttgat gatggtcgta tctatcaacg tccgtttggc
     1381 ggtcagtcga aaaacttcgg cggcgagcag gcggcacgca ctgcggcggc ggctgaccgt
     1441 accggtcacg cactgttgca cacgctttat cagcagaacc tgaaaaacca caccaccatt
     1501 ttctccgagt ggtatgcgct ggatctggtg aaaaaccagg atggcgcggt ggtgggttgt
     1561 accgcactgt gcatcgaaac cggtgaagtg gtttatttca aagcccgcgc taccgtgctg
     1621 gcgactggcg gggcagggcg tatttatcag tccaccacta acgcccacat taacactggc
     1681 gacggtgtcg gcatggctat ccgcgcaggc gtaccggtgc aggatatgga aatgtggcaa
     1741 ttccacccga ccggcattgc cggtgcgggc gtactggtca ccgaaggttg ccgtggtgaa
     1801 ggcggttatc tgctgaacaa acatggcgaa cgctttatgg agcgttatgc gccgaacgcc
     1861 aaagacctgg cgggccgtga tgtggtggcg cgttccatca tgattgaaat ccgtgaaggt
     1921 cgcggctgtg atggtccgtg ggggccacac gcgaaactga aactcgatca cctgggtaaa
     1981 gaagttctcg aatcccgtct gccgggtatc ctggagcttt cccgtacctt cgctcacgtc
     2041 gatccggtga aagagccgat tccggttatc ccaacctgtc actacatgat gggcggtatt
     2101 ccgaccaaag ttaccggtca ggcgctgact gtgaatgaga aaggcgaaga tgtggttgtt
     2161 ccgggactgt ttgccgttgg tgaaatcgct tgtgtatcgg tacacggcgc taaccgtctg
     2221 ggcggcaact cgctgctgga cctggtggtc tttggtcgcg cggcaggtct gcatctgcaa
     2281 gagtctatcg ccgagcaggg cgcactgcgc gatgccagcg agtctgatgt tgaagtgtct
     2341 ctggatcgcc tgaaccgctg gaacaataat cgtaacggtg aagatccggt ggcgattcgt
     2401 aaagcgctgc aagaatgtat gcagcataac ttctcggtct tccgtgaagg tgatgcgatg
     2461 gcgaaagggc ttgagcagtt gaaagtgatc cgcgagcgtc tgaaaaatgc ccgtctggat
     2521 gacacttcca gcgagttcaa cacccagcgc gttgagtgcc tggaactgga taacctgatg
     2581 gaaacggcgt atgcaacggc tgtttctgcc aacttccgta ccgaaagccg tggcgcacat
     2641 agccgcttcg acttcccgga tcgtgatgat gaaaactggc tgtgccactc cctgtatctg
     2701 ccagagtcgg aatccatgac gcgccgaagc gtcaacatgg aaccgaaact gcgcccggca
     2761 ttcccgccga agattcgtac ttactaatgc ggagacagga aaatgagact cgagttttca
     2821 atttatcgct ataacccgga tgttgatgat gctccgcgta tgcaggatta caccctggaa
     2881 gcggaagaag gtcgcgacat gatgctgctg gatgcgctta tccagctgaa agagaaagat
     2941 cccagcctgt cgttccgccg ctcctgccgt gaaggtgtgt gcggttccga cggtctgaac
     3001 atgaacggca agaatggtct ggcctgtatt accccgattt cggcactcaa ccagccgggc
     3061 aagaagattg tgattcgccc gctgccaggt ttaccggtga tccgcgattt ggtggtagac
     3121 atgggacaat tctatgcgca atatgacaaa attaagcctt acctgttgaa taatggacaa
     3181 aatccgccag ctcgcgagca tttacagatg ccagagcagc gcgaaaaact cgacgggctg
     3241 tatgaatgta ttctctgcgc atgttgttca acctcttgtc cgtctttctg gtggaatccc
     3301 gataagttta tcggcccggc aggcttgtta gcggcatatc gtttcctgat cgatagccgt
     3361 gataccgaga ctgacagccg cctcgacggt ttgagcgatg cattcagcgt attccgctgt
     3421 cacagcatca tgaactgcgt cagtgtatgt ccgaaggggc tgaacccgac gcgcgccatc
     3481 ggccatatta agtcgatgtt gttgcaacgt aatgcgtaaa ccgtaggcct gataagacgc
     3541 gcaagcgtcg catcaggcaa ccagtgccgg atgcggcgtg aacgccttat ccggcctaca
     3601 agtcattacc cgtaggcctg ataagcgcag cgcatcaggc gtaacaaaga aatgcaggaa
     3661 atctttaaaa actgcccctg acactaagac agtttttaaa ggttccttcg cgagccacta
     3721 catagacaag agctcgcaag tgaaccccgg cacgcacatc actgtgcgtg gtagtatcca
     3781 cggcgaagta agcataaaaa agatgcttaa gggatcacga tgcagaacag cgctttgaaa
     3841 gcctggttgg actcttctta cctctctggc gcaaaccaga gctggataga acagctctat
     3901 gaagacttct taaccgatcc tgactcggtt gacgctaact ggcgttcgac gttccagcag
     3961 ttacctggta cgggagtcaa accggatcaa ttccactctc aaacgcgtga atatttccgc
     4021 cgcctggcga aagacgcttc acgttactct tcaacgatct ccgaccctga caccaatgtg
     4081 aagcaggtta aagtcctgca gctcattaac gcataccgct tccgtggtca ccagcatgcg
     4141 aatctcgatc cgctgggact gtggcagcaa gataaagtgg ccgatctgga tccgtctttc
     4201 cacgatctga ccgaagcaga cttccaggag accttcaacg tcggttcatt tgccagcggc
     4261 aaagaaacca tgaaactcgg cgagctgctg gaagccctca agcaaaccta ctgtggcccg
     4321 attggtgccg agtatatgca cattaccagc accgaagaaa aacgctggat ccaacagcgt
     4381 atcgagtctg gtcgcgcgac tttcaatagc gaagagaaaa aacgcttctt aagcgaactg
     4441 accgccgctg aaggccttga acgttacctc ggcgcaaaat tccctggcgc aaaacgcttc
     4501 tcgctggaag gcggtgacgc gttaatcccg atgcttaaag agatgatccg ccacgctggc
     4561 aacagcggca cccgcgaagt ggttcttggg atggcgcacc gtggtcgtct gaacgtgctg
     4621 gtgaacgtgc tgggtaaaaa accgcaagac ttgttcgacg agtttgccgg taaacataaa
     4681 gaacacctcg gcacgggtga cgtgaaatac cacatgggct tctcgtctga cttccagacc
     4741 gatggcggcc tggtgcacct ggcgctggcg tttaacccgt ctcaccttga gattgtaagc
     4801 ccggtagtta tcggttctgt tcgtgcccgt ctggacagac ttgatgagcc gagcagcaac
     4861 aaagtgctgc caatcaccat ccacggtgac gccgcagtga ccgggcaggg cgtggttcag
     4921 gaaactctga acatgtcgaa agcgcgtggt tatgaagttg gcggtacggt acgtatcgtt
     4981 atcaacaacc aggttggttt caccacctct aatccgctgg atgctcgttc tacaccgtac
     5041 tgtactgata tcggtaagat ggttcaggca ccgattttcc acgttaacgc ggatgatccg
     5101 gaagccgttg cctttgtgac ccgtctggcg ctcgatttcc gtaacacctt taaacgtgat
     5161 gtcttcatcg acctggtgtg ctaccgccgt cacggccaca acgaagccga cgagccgagc
     5221 gcaacccagc cgctgatgta tcagaaaatc aaaaaacatc cgacaccgcg caaaatctac
     5281 gctgacaagc tggagcagga aaaagtggcg acgctggaag atgccaccga gatggttaac
     5341 ctgtaccgcg atgcgctgga tgctggcgat tgcgtagtgg cagagtggcg tccgatgaac
     5401 atgcactctt tcacctggtc gccgtacctc aaccacgaat gggacgaaga gtacccgaac
     5461 aaagttgaga tgaagcgcct gcaggagctg gcgaaacgca tcagcacggt gccggaagca
     5521 gttgaaatgc agtctcgcgt tgccaagatt tatggcgatc gccaggcgat ggctgccggt
     5581 gagaaactgt tcgactgggg cggtgcggaa aacctcgctt acgccacgct ggttgacgaa
     5641 ggcattccgg ttcgcctgtc gggtgaagac tccggtcgcg gtaccttctt ccaccgccac
     5701 gcggtgatcc acaaccagtc taacggttcc acttacacgc cgttgcaaca catccataac
     5761 gggcagggcg cgttccgcgt ctgggactcc gtactgtctg aagaagcagt gctggcgttt
     5821 gaatacggtt atgccaccgc agaaccacgc actctgacca tctgggaagc gcagttcggt
     5881 gacttcgcca acggtgcgca ggtggttatc gaccagttca tctcttctgg cgaacagaaa
     5941 tggggccgga tgtgtggtct ggtgatgttg ctgccgcacg gttacgaagg gcaggggccg
     6001 gagcactcct ccgcgcgtct ggaacgttat ctgcaacttt gtgctgagca aaacatgcag
     6061 gtgtgcgtac cgtctacccc ggcacaggtt taccacatgc tgcgtcgtca ggcgctgcgc
     6121 gggatgcgtc gtccgctggt cgtgatgtcg ccgaaatccc tgctgcgtca tccgctggcg
     6181 gtttccagcc tcgaagaact ggcgaacggc accttcctgc cagccatcgg tgaaatcgac
     6241 gagcttgatc cgaagggcgt gaagcgcgta gtgatgtgtt ctggtaaggt ttattacgac
     6301 ctgctggaac agcgtcgtaa gaacaatcaa cacgatgtcg ccattgtgcg tatcgagcaa
     6361 ctctacccgt tcccgcataa agcgatgcag gaagtgttgc agcagtttgc tcacgtcaag
     6421 gattttgtct ggtgccagga agagccgctc aaccagggcg catggtactg cagccagcat
     6481 catttccgtg aagtgattcc gtttggggct tctctgcgtt atgcaggccg cccggcctcc
     6541 gcctctccgg cggtagggta tatgtccgtt caccagaaac agcaacaaga tctggttaat
     6601 gacgcgctga acgtcgaata aataaaggat acacaatgag tagcgtagat attctggtcc
     6661 ctgacctgcc tgaatccgta gccgatgcca ccgtcgcaac ctggcataaa aaacccggcg
     6721 acgcagtcgt acgtgatgaa gtgctggtag aaatcgaaac tgacaaagtg gtactggaag
     6781 taccggcatc agcagacggc attctggatg cggttctgga agatgaaggt acaacggtaa
     6841 cgtctcgtca gatccttggt cgcctgcgtg aaggcaacag cgccggtaaa gaaaccagcg
     6901 ccaaatctga agagaaagcg tccactccgg cgcaacgcca gcaggcatct ctggaagagc
     6961 aaaacaacga tgcgttaagc ccggcgatcc gtcgcctgct ggctgaacac aatctcgacg
     7021 ccagcgccat taaaggcacc ggtgtgggtg gtcgtctgac tcgtgaagat gtggaaaaac
     7081 atctggcgaa agccccggcg aaagagtctg ctccggcagc ggctgctccg gcggcgcaac
     7141 cggctctggc tgcacgtagt gaaaaacgtg tcccgatgac tcgcctgcgt aagcgtgtgg
     7201 cagagcgtct gctggaagcg aaaaactcca ccgccatgct gaccacgttc aacgaagtca
     7261 acatgaagcc gattatggat ctgcgtaagc agtacggtga agcgtttgaa aaacgccacg
     7321 gcatccgtct gggctttatg tccttctacg tgaaagcggt ggttgaagcc ctgaaacgtt
     7381 acccggaagt gaacgcgtct atcgacggcg atgacgtggt ttaccacaac tatttcgacg
     7441 tcagcatggc ggtttctacg ccgcgcggcc tggtgacacc ggtactgcgt gatgtcgata
     7501 ccctcggcat ggcagacatc gagaagaaaa tcaaagagct ggcagtcaaa ggccgtgacg
     7561 gcaagctgac cgttgaagat ctgaccggtg gtaacttcac catcaccaac ggtggtgtgt
     7621 tcggttccct gatgtctacg ccgatcatca acccgccgca gagcgcaatt ctgggtatgc
     7681 acgctatcaa agatcgtccg atggcggtga atggtcaggt tgagatcctg ccgatgatgt
     7741 acctggcgct gtcctacgat caccgtctga tcgatggtcg cgaatccgtg ggcttcctgg
     7801 taacgatcaa agagttgctg gaagatccga cgcgtctgct gctggacgtg tagtagttta
     7861 agcttcacct gcactgtaga ccgacatgga tagggtgtcg gtctacggtt taaaagataa
     7921 cgattactga aggatggaca gaacacatga acttacatga atatcaggca aaacaacttt
     7981 ttgcccgcta tggcttacca gcaccggtgg gttatgcctg tactactccg cgcgaagcag
     8041 aagaagccgc ttcaaaaatc ggtgccggcc cgtgggtagt gaaatgtcag gttcacgctg
     8101 gtggccgcgg taaagcgggc ggtgtgaaag ttgtaaacag caaagaagac atccgtgctt
     8161 ttgcagaaaa ctggctcggc aagcgtctgg taacgtatca aacagatgcc aatggccaac
     8221 cggttaacca gattctggtt gaagcagcga ccgatatcgc taaagagctg tatctcggtg
     8281 ccgttgtaga ccgtagttcc cgtcgtgtgg tctttatggc ctccaccgaa ggcggcgtgg
     8341 aaatcgaaaa agtggcggaa gaaactccgc acctgatcca caaagttgcg cttgatccgc
     8401 tgactggccc gatgccgtat cagggacgcg agctggcgtt caaactgggt ctggaaggta
     8461 aactggttca gcagttcacc aaaatcttca tgggcctggc gaccattttc ctggagcgcg
     8521 acctggcgtt gatcgaaatc aacccgctgg tcatcaccaa acagggcgat ctgatttgcc
     8581 tcgacggcaa actgggcgct gacggcaacg cactgttccg ccagcctgat ctgcgcgaaa
     8641 tgcgtgacca gtcgcaggaa gatccgcgtg aagcacaggc tgcacagtgg gaactgaact
     8701 acgttgcgct ggacggtaac atcggttgta tggttaacgg cgcaggtctg gcgatgggta
     8761 cgatggacat cgttaaactg cacggcggcg aaccggctaa cttccttgac gttggcggcg
     8821 gcgcaaccaa agaacgtgta accgaagcgt tcaaaatcat cctctctgac gacaaagtga
     8881 aagccgttct ggttaacatc ttcggcggta tcgttcgttg cgacctgatc gctgacggta
     8941 tcatcggcgc ggtagcagaa gtgggtgtta acgtaccggt cgtggtacgt ctggaaggta
     9001 acaacgccga actcggcgcg aagaaactgg ctgacagcgg cctgaatatt attgcagcaa
     9061 aaggtctgac ggatgcagct cagcaggttg ttgccgcagt ggaggggaaa taatgtccat
     9121 tttaattgat aaaaacacca aggttatctg ccagggcttt actggtagcc aggggacttt
     9181 ccactcagaa caggccattg catacggcac taaaatggtt ggcggcgtaa ccccaggtaa
     9241 aggcggtact acccacctcg gcctgccggt gttcaacacc gtgcgtgaag ctgttgctgc
     9301 tactggcgct accgcttctg ttatctacgt accagcaccg ttctgcaaag actccattct
     9361 ggaagctatt gatgcaggca tcaaactgat tatcaccatc actgaaggca tcccgacgct
     9421 ggatatgctg actgtgaaag tgaaactgga cgaagccggc gttcgtatga tcggcccgaa
     9481 ctgcccgggc gttatcactc cgggtgaatg caaaatcggt atccagccgg gtcacattca
     9541 caaaccgggt aaagtgggta tcgtttcccg ttccggtaca ctgacctatg aagcggttaa
     9601 acagaccacg gattacggtt tcggtcagtc gacctgcgtt ggtatcggcg gtgacccgat
     9661 ccctggctct aactttatcg acattctcga aatgttcgaa aaagatccgc agaccgaagc
     9721 gatcgtaatg atcggtgaga tcggcggtag cgctgaagaa gaagcggcag cgtacatcaa
     9781 agagcatgtt accaagccag ttgtgggtta catcgctggt gtgactgcgc cgaaaggcaa
     9841 acgtatgggc cacgcgggtg ccatcattgc cggtgggaaa gggactgcgg atgagaaatt
     9901 cgctgctctg gaagccgcag gcgtgaaaac cgttcgcagc ctggcggata tcggtgaagc
     9961 actgaaaact gttctgaaat aaatatctgt aataagaaat agccctcgcc gcttccctct
    10021 acaggaatgg cgaagggctg tcggtttcga catggttggc catcgtatga tggccttttt
    10081 tgtgcttatc gcgatgattt tcgttgcgct atcagggtaa atttatagtc atcggtatta
    10141 aaagcgttgc ggctatattc aaacacccga ccatcaacta aatatccacg cgatactttt
    10201 tcaagaatcg gctttgtctg gctgatatta agcagacggc tcatctcttc ggttggcatc
    10261 agaggaatga tttcctgttc gctacgatcg ataaccattt tcttcacttc ttcaataaag
    10321 tgatattttg aattttccat gacctgccag gtgagatccg ggaacaacgc aagcggcatc
    10381 caggtttctt ccagcgccat tggcttttgc ttgcgatagc gcacgcgctt cacatgccac
    10441 acacgatcct gcgcggtgat ttgtagctgt tgctgaagaa aatcgtcagc cggaatcact
    10501 tcaaatatca ggacttcact gtgtgtatcg acgtgacggt ccgacagttt ttcatcaaaa
    10561 ctggttaact gaaaaatatc gtaattgacc cgctcttctt tgacgtaagt cccgctgccc
    10621 tgaatgcttt cgaggatctg ttgctcgact aactggcgca aagcctgacg caccgtaacc
    10681 cggctgacgc caaactctgt ttgtaatgcc gattcggtag gtaacgcatc gccaggttta
    10741 agctcgccac gcgcaatttg ttcacgaatg cgatcggcaa tctgccggta taagggcttg
    10801 tgtcccattt ttagtatctc attaatacga atttagccat tatgcccgat aaattcatcc
    10861 tgtaaataat acaaatacaa tacaaataat ttcaatcaag tgaaattgat tacataatgg
    10921 tattgtttta tcgggcacac tggcgcgact ataaaaacga tcaagtgagg atcatgatga
    10981 atctgacgac tctgacccac cgcgatgcgt tgtgtctgaa tgcgcgcttt accagccgtg
    11041 aagaggccat ccacgcgttg actcaacgtc ttgctgctct ggggaaaatt tccagtactg
    11101 agcaatttct ggaagaagtg tatcgccgtg aaagccttgg cccgaccgcc ttaggtgaag
    11161 ggttggctgt gccgcatggc aaaactgctg cggtaaaaga agcggcgttt gcggtcgcca
    11221 cactcagcga gccgcttcag tgggaaggcg ttgatggccc ggaagcggtt gatttagtgg
    11281 tgctgctggc gattcccccc aatgaagcgg gtactacgca tatgcaactg ctgacagcgc
    11341 tgaccacgcg cctggcggat gatgagattc gggcgcgtat acagtcggcg acgacgcctg
    11401 atgagttgct ctcggcgctg gatgacaagg gaggcacgca accttctgcc tctttttcca
    11461 acgcgccaac tatcgtctgc gtaacggcct gtccggcggg tattgctcac acctatatgg
    11521 ctgcggaata tctggaaaaa gccggacgca aactcggcgt aaatgtttac gttgaaaaac
    11581 aaggcgctaa cggcattgaa gggcgtttaa cggcggatca actcaatagt gcaaccgcct
    11641 gtatttttgc ggctgaagtc gccatcaagg agagtgagcg ttttaatggc attcccgcgc
    11701 tttcagtgcc tgttgccgag ccgattcgcc atgcagaagc gttgatccaa caagcgctta
    11761 ccctcaagcg tagcgatgag acgcgtaccg tacagcaaga tacgcaaccg gtgaaaagtg
    11821 tcaaaacgga gctgaaacag gcactgttga gcggaatctc ttttgccgta ccgttgattg
    11881 tcgcgggggg cacggtgctg gcggtcgcgg tattactgtc gcaaatcttc gggctacaag
    11941 atctgtttaa tgaagaaaac tcctggctgt ggatgtaccg caagctgggc ggcgggctgc
    12001 tcggaatttt gatggtaccg gtgctcgcgg cctataccgc ctattctctg gcagataaac
    12061 cggcgttagc gccaggcttt gcggctggac ttgccgccaa catgatcggc tccgggtttc
    12121 tcggcgcggt cgttggcgga ttgatagccg gttacttgat gcgctgggtg aaaaatcact
    12181 tgcgtcttgg cagtaaattc aatggcttcc tgacttttta tctctacccg gtgctcggta
    12241 cgttgggagc gggcagtctg atgctgtttg tggtgggaga acctgtcgca tggatcaata
    12301 actcgcttac cgcctggctg aacggtctgt caggaagtaa cgcgctgttg ctgggtgcca
    12361 ttctcggttt tatgtgttcc tttgaccttg gagggccagt gaataaagcc gcttatgcat
    12421 tctgcctggg cgcaatggcg aacggcgttt acggcccgta tgccattttc gcctccgtca
    12481 aaatggtttc ggcatttacc gtaaccgctt ccacgatgct cgcaccgcgc ctgtttaaag
    12541 agtttgaaat tgagaccggg aaatccacct ggctgttagg gctggcaggt attaccgaag
    12601 gggcgatccc gatggcgatt gaagatccgc tgcgggttat tggttcgttt gtgctgggct
    12661 ctatggtaac gggcgctatt gtcggtgcga tgaatatcgg cctttcgaca cccggtgccg
    12721 gcattttctc gctcttttta cttcatgata atggcgcggg cggtgttatg gcggcaattg
    12781 gctggtttgg tgcggcattg gtgggggctg caatctcgac tgcaattctc ctgatgtggc
    12841 ggcgtcacgc ggttaagcat ggcaactatc tgactgatgg cgtaatgcca taaacaaaaa
    12901 ggaaacgacg atgaaagcgg tatctcgcgt tcacatcacc ccgcatatgc actgggatcg
    12961 ggagtggtat ttcaccaccg aagagtcacg cattctgctg gtcaataata tggaagagat
    13021 cctgtgccga ctggaacagg acaacgaata caaatattac gtactcgacg ggcaaacggc
    13081 gatcctcgaa gattatttcg cggtgaaacc ggaaaacaaa gaccgtgtga agaaacaggt
    13141 agaagccggc aagttgatta tcggcccctg gtatacccag accgatacca cgattgtttc
    13201 tgcggaatcc atcgtccgta atctgatgta cggaatgcgt gactgcctcg cgtttggcga
    13261 gccgatgaaa ataggttatt taccggattc ctttggcatg tccgggcagc ttccgcatat
    13321 ctacaatgga tttggcatta cccgcaccat gttctggcgt ggatgttctg agcgccacgg
    13381 tactgataaa accgagtttt tgtggcaaag cagtgacggt agcgaagtga cggcgcaagt
    13441 gttgccgctg ggctacgcca tcggtaagta cttacctgcc gacgaagacg gattacgcaa
    13501 gcgcctcgac agttattttg atgtgctgga aaaagcgtca gtaaccaaag aaattttatt
    13561 gccgaatggt catgaccaga tgccattgca gcaaaatatc ttcgaagtga tggataagct
    13621 acgtgagatc taccctcaac gtaagtttgt gatgagccgc tttgaagagg tatttgagaa
    13681 gatcgaagcg cagcgagata atctggcaac cctgaaaggg gaatttattg atggcaaata
    13741 tatgcgcgtg catcgcacca tcggttctac gcgtatggat atcaaaattg cccacgcgcg
    13801 tattgaaaat aagattgtta atctgctgga accgctggca acactggcct ggacgttggg
    13861 ttttgaatac caccacggct tgctggagaa aatgtggaaa gagatcttaa aaaatcatgc
    13921 ccacgacagt atcggttgtt gttgcagtga caaagttcat cgcgaaatcg tcgcccgctt
    13981 cgaactggct gaagacatgg cggataatct gattcgtttc tacatgcgca aaattgccga
    14041 caacatgccg cagagcgacg ccgacaaact cgtcctgttt aacctgatgc cctggccgcg
    14101 tgaagaagtg atcaacacca ctgtgcgcct gcgcgccagc cagtttaatt tgcgggacga
    14161 tcgcggccag cctgtgccgt attttattcg ccatgcccgt gagatcgatc caggcctaat
    14221 cgatcggcaa attgttcatt atggcaatta tgatcccttt atggagttcg acatccagat
    14281 caaccagatt gtcccttcta tgggctatcg cacgctttat attgaagcga atcagcctgg
    14341 caacgtgatt gcggcaaaaa gtgacgctga agggattctg gaaaatgctt tctggcagat
    14401 tgcgctcaat gaggatggtt ctctgcaact ggtagataaa gacagcggtg tgcgctatga
    14461 ccgggtattg caaattgaag aaagctctga tgatggtgat gaatatgact attcacccgc
    14521 aaaagaagag tgggtaatta ccgcggcgaa cgcgaaaccg caatgcgata ttattcatga
    14581 agcctggcag agcagggctg ttatccgtta tgaaatggcg gtgccgcgta atctgcaaga
    14641 acgcagcgca agacaatcca ctggcagagt aggggtggag atggttgtca ctctgagtca
    14701 taacagcaga cgtattgatg tggatatcaa ccttgataac caggctgacg atcatcgcct
    14761 tcgcgtcctg atccctacac cttttaacac cgacagtgtt ctggcagata cgcagtttgg
    14821 ttcgctaacg cgccccgtga acgacagtgc aatgaacaac tggcagcaag aaggctggaa
    14881 agaagcgccg gttccggtat ggaatatgct caactatgtt gccttacagg aagggcgtaa
    14941 cggcatggct gtctttagcg aagggctacg tgaatttgaa gtcatcggtg aagagaagaa
    15001 aacctttgcc attacgttgc tgcgtggcgt gggcttactg ggcaaagaag atctgctttt
    15061 aaggcctggg cggccttcgg gaattaaaat gccagtcccg gactcacaac tacgtggtct
    15121 gctttcttgt cgcctaagtt tattgagtta taccggtacg ccaaccgccg ctggtgtagc
    15181 tcagcaggcg cgagcatggc tgactccagt acagtgttac aacaaaatcc catgggatgc
    15241 gatgaagctc aacaaagccg gattcaacgt gccggaaagt tatagtttgt tgaaaatgcc
    15301 cccagtggga tgcctgataa gcgcacttaa gaaagctgaa gaccgacaag aagtgatttt
    15361 acggctgttt aatccggctg aatcagcaac ctgtgatgcg actgttgctt tcagtcgcga
    15421 ggtgatttct tgctcagaaa cgatgatgga tgaacacatt accaccgagg aaaatcaagg
    15481 ttcaaatcta tcggggcctt ttttacccgg ccagtcacgg acgttcagtt accggcttgc
    15541 ctgaatagca atcaaaccga agccacatat gcgcggccag attgttgaca aagggcgctt
    15601 tgttcatgcc ggatacggca tgaacgcttt attcggtcta caaaagcagg caaattcaat
    15661 atattgcaga gatgatgtag acactggcaa gcgtagcgca tcaggtaatt ttgcgtttat
    15721 cttcactctc aagccacgta tatgtggctt tattttta
//
LOCUS       DAZFRJ010000102        15652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000102 DAZFRJ010000000
VERSION     DAZFRJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.102"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(468..1559)
                     /gene="ychF"
                     /locus_tag="ACHKUN_002923"
     CDS             complement(468..1559)
                     /gene="ychF"
                     /locus_tag="ACHKUN_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO6661145.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1676..2260)
                     /gene="pth"
                     /locus_tag="ACHKUN_002924"
     CDS             complement(1676..2260)
                     /gene="pth"
                     /locus_tag="ACHKUN_002924"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO6661146.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2538..2816
                     /gene="ychH"
                     /locus_tag="ACHKUN_002925"
     CDS             2538..2816
                     /gene="ychH"
                     /locus_tag="ACHKUN_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO6661147.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2871..4550)
                     /gene="dauA"
                     /locus_tag="ACHKUN_002926"
     CDS             complement(2871..4550)
                     /gene="dauA"
                     /locus_tag="ACHKUN_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO6661148.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4675..5622)
                     /gene="prs"
                     /locus_tag="ACHKUN_002927"
     CDS             complement(4675..5622)
                     /gene="prs"
                     /locus_tag="ACHKUN_002927"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO6661149.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5773..6624)
                     /gene="ispE"
                     /locus_tag="ACHKUN_002928"
     CDS             complement(5773..6624)
                     /gene="ispE"
                     /locus_tag="ACHKUN_002928"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO6661150.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6624..7247)
                     /gene="lolB"
                     /locus_tag="ACHKUN_002929"
     CDS             complement(6624..7247)
                     /gene="lolB"
                     /locus_tag="ACHKUN_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO6661151.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7461..8717
                     /gene="hemA"
                     /locus_tag="ACHKUN_002930"
     CDS             7461..8717
                     /gene="hemA"
                     /locus_tag="ACHKUN_002930"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO6661152.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8759..9841
                     /gene="prfA"
                     /locus_tag="ACHKUN_002931"
     CDS             8759..9841
                     /gene="prfA"
                     /locus_tag="ACHKUN_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO6661153.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9841..10674
                     /gene="prmC"
                     /locus_tag="ACHKUN_002932"
     CDS             9841..10674
                     /gene="prmC"
                     /locus_tag="ACHKUN_002932"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6661154.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10671..11063
                     /gene="sirB2"
                     /locus_tag="ACHKUN_002933"
     CDS             10671..11063
                     /gene="sirB2"
                     /locus_tag="ACHKUN_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO6661155.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11067..11876
                     /gene="sirB1"
                     /locus_tag="ACHKUN_002934"
     CDS             11067..11876
                     /gene="sirB1"
                     /locus_tag="ACHKUN_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO6661156.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11912..12766
                     /gene="kdsA"
                     /locus_tag="ACHKUN_002935"
     CDS             11912..12766
                     /gene="kdsA"
                     /locus_tag="ACHKUN_002935"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO6661157.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12915..13022)
                     /locus_tag="ACHKUN_002936"
     CDS             complement(12915..13022)
                     /locus_tag="ACHKUN_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6661158.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13450..13557)
                     /locus_tag="ACHKUN_002937"
     CDS             complement(13450..13557)
                     /locus_tag="ACHKUN_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6661159.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
     gene            complement(13986..14093)
                     /locus_tag="ACHKUN_002938"
     CDS             complement(13986..14093)
                     /locus_tag="ACHKUN_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6661160.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(14498..15598)
                     /gene="chaA"
                     /locus_tag="ACHKUN_002939"
     CDS             complement(14498..15598)
                     /gene="chaA"
                     /locus_tag="ACHKUN_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO6661161.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3931 a         3957 c         3998 g         3766 t
ORIGIN      
        1 ccatttttgc attactaata agaaaaagca aaaaacatcg atattcattt ttggtcaatg
       61 ggttttctgg cctatcgttg ttattgtgct actggttatc attttcatct tgccaggatc
      121 gcacgtaatc acgttattcg gggactttat ctgcgggata ttacccttca ggtaagtcct
      181 tttctcgcca gcatattgga gcgtgatcaa tattgatcag ctgtgaaggg ggtgatgtgt
      241 agtggaccaa aacttacgtt atatcactac taatgagata tccgtcatct gacactgatg
      301 tctttaatta ttctcgtggt tcactgtagg caataagcac aaaagtgtag gatgttacaa
      361 gaatgattag gactcggtga aatgaaaaat ccacgcaatt gcgtggatta tatatacttt
      421 tgcgctcttc atgagattta gcgaaacctc atgagacaac aaattaatta gacgttgaaa
      481 aggaagttca tcacatcgcc atctttaacg atgtaatctt taccttctgc acgcattttg
      541 cctgcttctt tcgcgccttg ttcacctttg taagtgatga aatcttcaaa cgagatggtt
      601 tgtgcacgga taaagccttt ttcaaaatca gtatggattt tgcccgctgc ctgcggcgcg
      661 gttgctccaa ccggaatggt ccatgcacgc acttctttca ccccagcggt gaagtaagtt
      721 tgcaggttca gcagcttata accggcacgg atcacacggt tcaggcccgg ctcttccagc
      781 ccaagctcct gcataaactc gtcacgttct tcgtcgtcca gttcggcaat gtctgcttca
      841 acagcagcac aaaccggaac cacaacagaa ccttctttcg ccgcgatttc acgcacctgg
      901 tcaagatatg ggttgttttc aaaaccgtct tcgttgacgt tggcgatgta cattgttggt
      961 ttcagcgtca ggaagctcag gtaacgaata gccgctttct cttcagcgct taaatccagc
     1021 gcgcgcagca tacctgcatt ttccaactgg ggcaggcatt tttccaggac cgccagctca
     1081 gctttcgcgt ctttatcgcc acctttggct ttcttctgta cgcgatgaat cgcacgttcg
     1141 caggtgtcga ggtctgccag cgccagttcg gtgttgataa cttcaatatc gtcagccggg
     1201 ttaactttgc cggaaacgtg aatgatgttg tcattttcaa agcagcgaac aacgtgaccg
     1261 atcgcttcgg tttcacggat gttggtcagg aactggttac ccagaccttc gcctttcgat
     1321 gcgcctttta ccagaccggc gatatcgaca aattccatgg tcgtgggaag cgtacgttgc
     1381 ggttttacga tttcagccag ttgatctagg cgagggtcag gcattggtac gacgcctgtg
     1441 ttcggctcaa tggtgcagaa tggaaagttg gccgcttcaa taccggcttt ggtcagcgcg
     1501 ttgaacaggg tagatttccc gacgttgggc aaaccgacga taccgcattt gaatcccatg
     1561 atttaaatca ccttaatatc ttaataatca acctgttatt gctaacagat tgcagaaatg
     1621 gaaataactt tgcctattat acacggcact cggcaaaaat gccgcagaca acgacttatt
     1681 gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa gccatctgta aaccacattt
     1741 cagtacaacg cgccgcttcg tcgatggctt catcaattaa cttctgttca ctaacaggcg
     1801 gtttgcctaa cacaaaaccg acaactttat ttttatcgcc cggatgaccg attccgatgc
     1861 gtaaacggtg aaagttaggg ttattaccca atttactgat gatgtctttc agtccattgt
     1921 gaccaccatg gccaccgccc aatttaaatt tggcgacgcc aggaggcaga tccagttcgt
     1981 cgtgggccac cagaatttcg tccggattaa tgcggaaaaa actggccatc gccgcaacgg
     2041 ctttgccgct gagattcata aatgtagtcg ggactaacag gcggacatct tcgcctccaa
     2101 gagtgactcg cgaagtataa ccaaagaatt tagcctcttc gcgcagcgga gcgcgcaaac
     2161 gctctgccag taagtcaacg aaccaggcac cagcattatg tcgcgttgcg gcgtattcag
     2221 caccggggtt cgccaggccg acaatcaatt taatcgtcac gtttttttgt cctgagtgtg
     2281 tacataactg gcgcgtagtt tactggttgc ggccccgctt gacaaaaaac tgcgtatcaa
     2341 atgcagataa cgtaataatt gcatgagtgg actattagaa agtcaaggtg ttcaggcgtt
     2401 tatttgtaaa gttttgttga aataagggtt gtaattgtga tcacgcccgc acataaccca
     2461 cagggtgttg tctatacttt acacataagg aagaggggta ttccctgtta caacccagaa
     2521 agttccggag gtgacatatg aaacgcaaaa acgcttcgtt actcggtaac gtgctcatgg
     2581 ggttgggtct ggtggtaatg gtggtcggcg tggggtattc aatcctcaac cagttaccac
     2641 agtttaatat gccccagtat ttcgcacatg gtgcagtgct aagtattttc gtcggtgcca
     2701 ttctctggct ggcgggtgcc cgtgttggcg ggcatgaaca ggtgtgcgac cgttactggt
     2761 gggttcgcca ctatgacaaa cgttgccgcc gtagcgataa tcgccgtcat agctaacaaa
     2821 taatgcagtt tgctgaccag tcagtttgcg ctgactggtc aatctcgtac ttataaatcc
     2881 gccatcgccg cgcgacgatt cgggaagaac gccaggcgtc ccgggatcgg ttgaatgcca
     2941 gcgcgagcca tagtgcgcag tggctggaat tccacgttgc acacgcgcag ttcacatccc
     3001 tcgggcagac gcttcacaaa acgctggaac gcatcaagac caccagcatc aagtaccgga
     3061 accgcatccc acttcagaat cacaatccgt ttgccttcaa gacgtgactc caggtccgtg
     3121 aacaagcctt cagcagcagc aaaaaacagc gggccaataa cgcgcagaac caggacatcg
     3181 tctggaacat ctacgactac cggtgccagg cgagtcatac gtgcgatacg acgcataaac
     3241 agcagcgatg ccagcacgat ccccacgctg atggcaataa ccatatcaaa cagcacggtc
     3301 agcgacatgc acagcagcat gacaatgatg tcatctttcg gcgcatgacg cagcaagtcg
     3361 accactttgt gcgcttcact catgttccac gccaccatca acagcagggc agccatggcg
     3421 gaaagcggca gccaggagag cagcggtgcc agtaccagca gggcaagaat aaccagaata
     3481 gagtggatca ccgccgagat aggggaagtt gccccggcac ggacgttagc ggcagaacgc
     3541 gcgatggcag ctgtagcggt aatgccacca aagaacggag cgatgatatt ccccagtccc
     3601 tgtccaacca gttcgctatt cgccttgtgt ttcgtcccgg tcataccatc cagcaccacg
     3661 gcgcagagca gagattcgat tgcgccgagc attgccattg agaatgccgc aggcagcagt
     3721 gtgcgaatag aatcccaggt tagcgtgaat tctgaattag gcagatccca cggcagcacc
     3781 agttgcggca gcagttgcgg aataccgtta ccctgagaac catcggccag gacgtagtgg
     3841 aattgcgaac cgatggtagc aacatgtccg ccgagcaggt taacaatccc catcaccgcg
     3901 caaccagcca gcaaggccgg aaggtgaccg ggtaaacgaa tgcccagacg cggccagaaa
     3961 acaagaatac ctagcgtcac aatgccaatg gcagcatcac ccacattaat ggtcggcagc
     4021 gccataaata atgcgccgac tttttgtaga taatgttccg ggacatgggc catttgcaga
     4081 ccgagaaaat ctttaatctg catggtaccg atggtgatcc caatacccga ggtgaaacct
     4141 aaggtgacgg aaaccggaat atactcaatc aggcgaccaa agcgtgccag acccatcaga
     4201 atcaaaaaaa tccccgacag caaggtcgca accagcagtc ctgccagtcc aaactgttgc
     4261 gacacgggat agagaattac cacaaatgcc gcagtcggac cggaaacgct aaagcgtgac
     4321 ccacccgtca gagcaatgac aatccccgca acagctgcgg tatataaacc gtactggggt
     4381 gccacaccac taccaatagc caacgccatc gccagcggga tagcaataat cccgacggtt
     4441 atcccggcaa tcaggtcacg ggtaaaccgt gcggcagtat atttttcttt ccagcaagcg
     4501 tcgatcagag cgcggaaagg catcacatgt gaggaaaata ttttgttcac aataatgttt
     4561 catccgtgag cgcatcatct gtcaactaaa tggcaggtga aggaggcata ggtcatacaa
     4621 atggatatta cagacaaaaa aacccgccgc agcgggtctt tgagccgggt tcgattagtg
     4681 ttcgaacatg gcagagatcg attcttcgtt gctgatacga cgaatcgctt cggccagcat
     4741 acctgacagg gtcagagtac gcacgttcgg cagtgatttg atttcatcgc tcagcggaat
     4801 ggtatcgcag acaacgactt catcaattac agagttacgc aggttattcg ccgcgttgcc
     4861 agagaagatc gggtgagtcg cgtacgcaaa tacacgttta gcaccacgtt ctttcagagc
     4921 ttcagcagct ttacacagcg taccgccagt gtcgatcata tcatcgacca gtacgcagtc
     4981 acgacctgca acgtcaccga tgatatgcat cacctgggaa acgttcgcac gcggacgacg
     5041 tttgtcgatg attgccatat cggtatcgtt cagcagctta gcgatagcgc gggcacgcac
     5101 aacgccgccg atgtccggag aaaccacaat tgggttatcc agattcagct gcagcatgtc
     5161 ttccagcagg atcgggctac caaatacgtt atcaaccgga acgtcgaaga aaccctgaat
     5221 ctgttcagcg tgcagatcca ctgtcagcac acggtcaaca ccgacgctgg agaggaagtc
     5281 tgcaaccact ttcgcagtga ttggtacacg agcggaacgg acgcgacggt cctggcgcgc
     5341 atagccaaag taggggataa cagcggtgat acgacctgcg gaagcacgac gcagggcatc
     5401 aaccataacg actaattcca tcaggttgtc gttagtaggg gcacaagtgg actggatgat
     5461 gaaaatatca ccaccgcgta cattttcgtt gatttgtaca ctgacttcgc cgtcgctaaa
     5521 gcgacctaca gcggcgtcgc cgagtgaagt gtacaggcgg ttggcaatac gttgtgctag
     5581 ttccggggtg gcgttaccag caaaaagctt catatcaggc acgagaagaa cctcaggcat
     5641 gcgtccattg gtggaaagaa tctgccgaaa actgtgcggg ccaggcatga tcctttccag
     5701 gcggtgtatt aaagagcgcg atgcaacgtc tggaacaagg tgacgttgtc accgaaactc
     5761 agcttgcccg gcttaaagca tggctctgtg caatggggaa agattaacgc ctttcgccac
     5821 aaagccattg agccattccg gggcttgctc tagcacctgg cgggcttcag actctgtatc
     5881 aaattcagca aagacacagg cccctgtccc agtcaggcgc gacggggcgt attctaacag
     5941 ccaggaaagc accgcatcaa cctcgcgaaa acgttttctt gcgataacct cgcaatcatt
     6001 gctgaattca cattttagca acgtttctat tgaccttttt ggcgtattgc gcgggagttc
     6061 aggatcttta aaaatcaccg gagtcggaat acttacacca gggtgcgcca ccagatacca
     6121 cttctctggc ggatccaccg gcgttagtat ttcaccaacg ccttcggcaa acgaggcatg
     6181 cccccgaaca aagacaggaa catctgcgcc cagcgtcagc cccatttccg ccagctcatc
     6241 catgcttagc ccgcattgcc agagatgatt taatgccacc aggaccgtcg cggcattgga
     6301 tgaaccaccg ccgagaccgc cgcccatcgg caaacgcttg tcaatgctga tattcgcacc
     6361 gcttcccgtc ggaagacgcc cgctgtctgc cgcagttttc atcaacaatc gcgctgcgcg
     6421 aacgatcagg ttatcttcat gttccacgcc ttcaacgggc gttaacagac gaatatcccc
     6481 atcgtcacga agctcaatgc tgatggtgtc gccgtaatca agaaactgaa acagcgtttg
     6541 cagcgtgtgg taaccatccg cacgctgacc ggtaatgtat aaaaacagat taagttttgc
     6601 cggagagggc cactgtgtcc gcattatttc actatccagt tatccatttt taacttgatg
     6661 cgttgaccac cgtcggtgag ttccatattg gctggcatcg caggttgcgt tttggtgtca
     6721 taaccaccat aaacaacctt ccagtttttg ccattctggc tgtaggtaat ttcgctcagg
     6781 cggtactggt cgtccagttt gtagtcggtt gcatcacccg gtaaacctaa aatccactgg
     6841 cgcaagctgt tgagcggaat tggcattccg gtcaatttgc caatcatctc ttcggcgtca
     6901 tcggcggtat aacgctgacc tttattgtcg actaactgca cgttacccgg ttgagcattc
     6961 agctccagtt ccgtgctgcc caatgggtta gtgagcagca gacggtagcg atcctggccg
     7021 gtttgctgcc agaaaaagcg ggcgtacact ttttgttggt cagaaatata agcgaacgcg
     7081 ccgcgagtct gatactgatt aagattgcgc acgtcttgct gatgctgacg ccattgtggc
     7141 gaatccgggc ttttgccagg acctttgggc gtggtaacgg aacaggcagt gagcacaaga
     7201 gcagccagcg gtagcaggcg gataagacga aaatcgggca ggggcatagt gatgacaagt
     7261 ccttgagata cgttgcagtt ataaccctta atgctagcgt taccgtccgc tatcgtctat
     7321 gttcaagttg tcttaattgc cagaatctaa cggctttcgg caattactcc aaaagggggc
     7381 gctctctttt attgatctta cgcatcctgt atgatgcaag cagactaacc ctatcaacgt
     7441 tggtattatt tcccgcagac atgacccttt tagcactcgg tatcaaccat aaaacggcac
     7501 ctgtatcgct gcgagaacgt gtatcgtttt cgccggataa gctcgatcag gcgcttgaca
     7561 gcctgcttgc gcagccgatg gtgcagggcg gcgtggtgct gtcgacgtgc aaccgcacgg
     7621 aactttatct tagcgttgaa gagcaggata acctgcaaga ggcgttaatc cgctggcttt
     7681 gcgattatca caatcttaat gaagaagatc tgcgtaaaag cctctactgg catcaggata
     7741 acgacgcggt tagccattta atgcgtgttg ccagcggcct agattcactg gttcttgggg
     7801 agccgcagat cctcggtcag gttaaaaaag cgtttgccga ttcgcaaaaa ggtcatatga
     7861 aggccagcga actggaacgc atgttccaga aatctttctc tgtagcgaaa cgcgttcgca
     7921 ctgaaacaga tatcggtgcc agtgctgtgt ctgtcgcttt tgcggcttgt acgctggcgc
     7981 ggcagatctt tgaatcgctc tctacggtca cagtgttgct ggtaggcgcg ggcgaaacca
     8041 tcgagctggt agcgcgtcat ctgcgcgaac ataaagtaca gaagatgatt atcgccaacc
     8101 gcactcgcga acgtgcccaa attctggcag atgaagttgg cgcggaagtg attgccctga
     8161 gtgagatcga cgaacgtctg cgcgaagccg atatcatcat cagttccacc gccagcccgt
     8221 taccgattat cgggaaaggc atggtggagc gcgcattaaa aagccgtcgc aaccaaccaa
     8281 tgctgttggt ggatattgcc gttccgcgcg atgttgagcc ggaagttggc aaactggcga
     8341 atgcttatct ttatagtgtg gacgatctgc aaagcatcat ttcgcacaac ctggcgcagc
     8401 gtaaagccgc agcggttgag gcggaaacta ttgtcgctca ggaaaccagc gaatttatgg
     8461 cgtggctgcg agcacaaagc gccagcgaaa ccattcgcga gtatcgcagc caggcagagc
     8521 acgttcgcga tgagttaacc gccaaagcgt tagcggccct tgagcagggc ggcgacgcgc
     8581 aagccattat gcaggatctg gcatggaaac tgactaaccg cttgatccat gcgccaacga
     8641 aatcacttca acaggccgcc cgtgacgggg ataacgaacg cctgaatatt ctgcgcgaca
     8701 gcctcgggct ggagtagcag tacatcattt tcttttttta cagggtgcat ttacgcctat
     8761 gaagccttct atcgttgcca aactggaagc cctgcatgaa cgccatgaag aagttcaggc
     8821 gttgctgggt gacgcgcaaa ctatcgccga ccaggaacgt tttcgcgcat tatcacgcga
     8881 atatgcgcag ttaagtgatg tttcgcgctg ttttaccgac tggcaacagg ttcaggaaga
     8941 tatcgaaacc gcacagatga tgctcgatga tcctgaaatg cgtgagatgg cgcaggatga
     9001 actgcgcgaa gctaaagaaa aaagcgagca actggaacag caattacagg ttctgttact
     9061 gccaaaagat cctgatgacg aacgtaacgc cttcctcgaa gtccgagccg gaaccggcgg
     9121 cgacgaagcg gcgctgttcg caggcgatct gttccgtatg tacagccgtt acgccgaagc
     9181 ccgccgctgg cgggtagaaa tcatgagcgc cagcgagggt gaacatggtg gttataaaga
     9241 gatcatcgcc aaaattagcg gtgatggtgt gtatggtcgt ctgaaatttg aatctggcgg
     9301 tcatcgcgtg cagcgtgttc ctgctacgga atcgcagggt cgtattcata cttctgcttg
     9361 taccgttgcg gtaatgccag aactgcctga cgcagaactg ccggacatca acccagcaga
     9421 tttacgcatt gatactttcc gctcgtcagg ggcgggtggt cagcacgtta acaccaccga
     9481 ttcggcaatt cgtattactc acttgccgac cgggattgtt gttgaatgtc aggatgaacg
     9541 ttcacaacat aaaaacaaag cgaaggcgct ttcagtgctc ggtgctcgca tccacgctgc
     9601 tgaaatggca aaacgccaac aggccgaagc gtctacccgt cgtaacctgc tggggagtgg
     9661 cgatcgcagc gaccgtaacc gtacttacaa tttcccgcag gggcgcgtta ccgatcaccg
     9721 catcaacctg acgctctacc gcctggatga agtgatggaa ggtaagctgg atatgctgat
     9781 tgaaccgatt atccaggaac atcaggccga ccaactggcg gcgttgtccg agcaggaata
     9841 atggaatatc aacactggtt acgtgaagca ataagccaac ttcaggcgag cgaaagcccg
     9901 cggcgtgatg ctgaaatcct gctggagcat gttaccggca gagggcgtac ttttattctc
     9961 gcctttggtg aaacgcagct gactgacgaa caatgtcagc aacttgatgc gctactgaca
    10021 cgtcgtcgcg atggtgaacc cattgctcat ttaaccgggg tgcgagaatt ctggtcgttg
    10081 ccgttatttg tttcgccagc gaccttaatt ccgcgcccgg atacggagtg tctggtggag
    10141 caggcactgg cgcggttgcc tgaacaacct tgccgtattc ttgatctcgg gacgggtacc
    10201 ggggcgattg cgcttgcgct ggctagcgag cgcccggact gcgaaattat cgctgtagat
    10261 cgtatgcctg atgctgtctc cctggcacaa cgtaatgccc agcatctggc gatcaaaaat
    10321 atccacattc tgcaaagcga ctggtttagc gcgctagccg ggcagcagtt tgcgatgatt
    10381 gtcagcaatc cgccgtatat tgacgagcag gacccacatc ttcaacaagg cgatgtccgc
    10441 tttgagccgc tcactgcgct ggttgcggca gacagtggaa tggcagacat cgtgcatatc
    10501 atcgaacagt cgcgtaacgc gctggtatcc ggcggctttc tgcttctgga acatggctgg
    10561 cagcagggcg aagcggtgcg acaagcattt atcctcgcgg ggtatcatga cgtcgaaacc
    10621 tgccgtgact atggtgataa cgagcgcgta acgctcggcc gctattatca atgacaagtt
    10681 tttctacact gcttagtgtt catcttatta gtatcgcgct ttctgttggg ctattaacct
    10741 tacgtttctg gctacgttat cagaagcatc cacaggcatt tgctcgctgg acgcgcattg
    10801 tgccgccggt tgtcgatacg gtgttattgt taagcggcat tgcgttgatg gctaaagcgc
    10861 acatcctgcc attttccggg caggcacagt ggctgactga aaagctgttt ggagttatca
    10921 tttatatcgt tttgggtttt attgcactcg attatcgtcg tatgcacagt cagcaggcgc
    10981 gcattattgc cttcccgctg gcgttggtgg tgctgtacat catcattaaa ctcgccacca
    11041 caaaagtacc gttactgggg taagtcatga gatcgttagc tgatttcgaa tttaataaag
    11101 cgccattgtg cgaaggcatg atcctggctt gcgaagcaat ccgccgcgat tttccctcgc
    11161 aagatgttta cgacgaactg gagcgtctcg ttagtctggc gaaggaagaa atcagccagc
    11221 ttctgccttt agaagagcag ctggaaaaac tgatcgcgct gttttacggc gactggggat
    11281 ttaaagcctc acgcggcgtt tatcgtcttt ccgatgcatt atggctggac caggtgttaa
    11341 agaatcgaca gggcagtgcg gtatcattag gtgcggtttt attatgggtc gcgaatcgtc
    11401 tcgatttgcc gctgctgccg gtgattttcc ctacgcagct gatattgcgc attgaatgtc
    11461 cggatggcga aatttggctg attaatcctt ttaacggtga atcgttaagc gaacatatgc
    11521 tggacgtatg gttaaaggga aatatcagcc cgtcggcgga actgttttat gaagaccttg
    11581 atgaagctga taacattgag gtaatccgca aattgctgga tacactcaaa gcctcgttga
    11641 tggaagaaaa tcagatggag ctggcgttac gcaccagcga agctttatta caattcaacc
    11701 ctgaagatcc ctatgaaatt cgcgatcgcg ggttgattta tgtgcaactg gattgcgaac
    11761 acgttgcgtt gaacgattta agttatttcg ttgaacagtg tccggaagac ccgatcagcg
    11821 aaatgatccg tgcgcaaata aataacatcg cgcataaaca tattgtgctg cattaattaa
    11881 tcgacatttt actcaagatt aaggcgatcc tatgaaacaa aaagtggtta gcattggcga
    11941 catcaacgta gcaaatgacc tgccgttcgt actgtttggc ggtatgaacg tgctggaatc
    12001 tcgcgatctg gcgatgcgca tttgcgagca ctacgtaact gtgacccaga aactgggtat
    12061 cccttacgtg ttcaaagcct cttttgacaa agccaaccgc tcctccatcc actcttatcg
    12121 tggaccgggc ctggaagaag ggatgaaaat cttccaggag ttgaagcaga cttttggcgt
    12181 gaaaattatc accgacgttc acgaaccaag tcaggcacag cccgttgctg atgtcgtgga
    12241 tgtgattcag ttgccggcgt ttcttgctcg ccagactgac ctggttgaag ccatggcgaa
    12301 aaccggtgcg gtaattaacg tcaagaaacc acagtttgtc agcccgggac agatgggtaa
    12361 tatcgttgat aaattcaaag aaggcggcaa cgaaaaagtg attctttgcg atcgtggtgc
    12421 taacttcggc tatgacaacc tggttgtcga tatgctgggc ttcagcatta tgaagaaagt
    12481 gtctggtaac tcgccggtga ttttcgacgt gacccacgca ctgcaatgcc gcgatccgtt
    12541 tggcgcagct tccggtggtc gtcgtgctca ggtggctgag ctggcacgag ccggtatggc
    12601 ggtaggtctg gcggggctgt ttattgaagc gcatccggat ccggaacatg cgaaatgtga
    12661 tggtccatcc gcgctgccgc tggctaaact ggaaccgttc ctcaagcaga tgaaagcgat
    12721 tgatgatctg gtgaaaggtt tcgaagaact ggataccagc aagtaatctt ttttgcttga
    12781 aaaataaagt cttagcgttc tgcgttaaga cttttttcat gggtgccaga tacaaaaaag
    12841 gccgcaggct gttacccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga
    12901 cgcccgtttt caccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt
    12961 cccgccagga tcggtgctgc caggtcgtgc cagaaaatca tggcaaactg cgcgagcgtc
    13021 atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaattg ttctggttca
    13081 agattagccc ccgttctgtt gtcaggttaa acctctcaac gtgcgggggt tttctctttc
    13141 cagcaaccaa tgccaccagg gataaagccc ccgcaacatt gcgcctcacc ggataacgcc
    13201 ggcttgatgt ggatactacg tctcaattca tcttcacttc atccctgaaa tgtttgcaat
    13261 gaagagtgca ttccggtttt tcaacagctg ttacagtcgt ttcatgaggg ctctggatga
    13321 tgcttccagc tcgggttgcc aatgtttact ggcggaagag ataaagacaa aaaaggccgc
    13381 aggctgttac ccctgcggcc ggtttcgggc gcatattgcc atcacggcag cctgacgccc
    13441 gttttcgcct tacttccggt tacgccacca gccgacaatc gctgcggtaa taattcccgc
    13501 caggatcgat gctgccaggt cgtgccagaa aatcatggca aactgcgcga gcgtcatata
    13561 gccgccttgt tgtaatgaca acattttgcg gctattcttg aattgttgat ggttcaagat
    13621 tagcccccgt tctgttgtca ggttttacct ctcaacgtgc gggggttttc tctttccagc
    13681 aaccaatgcc accagggata aagcccccgc aacattgcgc ctcaccggat aacgccggct
    13741 tggtgtggat actacgactc aattcatctt cacttcatcc ctgaaatgtt tgcaatgaag
    13801 agtgcattcc ggtttttcaa cagctgttac agtcgtttca tgagtgctct ggatgatgct
    13861 tccagctcgg gttgccaata tttacttgtg gaagagataa agacaaaaat ggccgcaggc
    13921 tgttacccct gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt
    13981 tcaccttact tccggttacg ccaccagctg acaatcgctg cggtaataat tcccgccagg
    14041 atcggtgctg ccaggtcgtg ccagaaaatc atggcaaact gcgcgagcgt catatagccg
    14101 ccttgttgta atgacaacat tttgcggcta ttcttgaagt gtctggtttc aagattagcc
    14161 cccgttttgt tgtcaggttt tacctctcaa cgtgcggggg ttttctcttt ccagcaacca
    14221 atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc cggcttggtg
    14281 tggatactac gtcgcaattc atcttcactt catccctgaa atgtttgcaa taaagagtac
    14341 attccggctt ttcaacagct gttgcagtgg tttcgtgcag gttgtggtac aggctcgcaa
    14401 ttctgattaa cgagctgtcc aggtagtatg aagaaataca ataagcagga atttatctta
    14461 tctcccccga tttccgtcat cggaggagat ataactgtca ggcaaatatc gtcatcaaat
    14521 aggcggcaaa cagtgccaga tgcgctgcgc cattgagcac gttagtacgt ccggtggaga
    14581 aggagatatg gcacagcact aaagaggcca ccatcaccac catttctggc gcaccaagtg
    14641 caaactgtaa ttcgttaccc gtcataaagg caattagcgt gacgacaggt acggtaagcg
    14701 aaatggttgc taacactgaa ccaaagaaca gattcatcgc gcgctggacc tggttgttca
    14761 acactgcttt taacgcacct aaaccttccg gcgacagaat caacagagcc accaggaagc
    14821 cagtaaaggc gacaggggca ttcatgctgt cgagcaatgt ctccagcggg ctggcattca
    14881 ttttggtgac tgcaataacg gcaatcagat ggataatcaa ccagatagca tgccacacgc
    14941 tgctatgggc agacggttta ccgtgatgcg ggtcatcgtc atcactgtca tcttcgtgct
    15001 cgtagacaaa caaactttga tgcgttttgg tctggatcag caaaaatacg ccatacattg
    15061 ccgcagaaat taatgctacc agtaacgcct gaccggttga aaaattcgcc gcaggcagag
    15121 ccattggaaa taccagtacg attatcgcca gggggaacag cgcaattaaa tactgcttga
    15181 taccaaacag attcatatat tgggtggcaa acttacgacc gcccaacaat aatgaaaagc
    15241 caaccagccc accggtaaca atcataatga ttgaatagag cgtatcacgc attagcgttg
    15301 gcgcggcgtc gccggttgcc attaaagctg aaatcaaact gacttcaaga ataaccactg
    15361 aaaggctaag aataagcgaa ccgtaaggtt ctcccaggcg atgggctaat acgtccgcat
    15421 gacggacaac actaaaggcg ctacttaaaa taccaataag cgcaagaaga ttgatggcaa
    15481 tgaccactgg tagtgtctgg ctgcttcccc acaggaacag cactaccagc gccagaaccg
    15541 ggaaaataag cgaagtctcc ttgtggcggg tttttaccgc ctcttgagca tttgacatta
    15601 tggttatccc tttgcagatg aatttatcga aaatgtaaaa aataggtagg aa
//
LOCUS       DAZFRJ010000103        15377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000103 DAZFRJ010000000
VERSION     DAZFRJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.103"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..699
                     /gene="yceJ"
                     /locus_tag="ACHKUN_002940"
     CDS             133..699
                     /gene="yceJ"
                     /locus_tag="ACHKUN_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6661162.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            703..1278
                     /gene="yceI"
                     /locus_tag="ACHKUN_002941"
     CDS             703..1278
                     /gene="yceI"
                     /locus_tag="ACHKUN_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO6661163.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1320..2372)
                     /gene="trhO"
                     /locus_tag="ACHKUN_002942"
     CDS             complement(1320..2372)
                     /gene="trhO"
                     /locus_tag="ACHKUN_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO6661164.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2597..3517
                     /gene="lpxL"
                     /locus_tag="ACHKUN_002943"
     CDS             2597..3517
                     /gene="lpxL"
                     /locus_tag="ACHKUN_002943"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO6661165.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPQQYMWLHRRFKTRPEGVPSRY"
     gene            3689..4915
                     /gene="mdtG"
                     /locus_tag="ACHKUN_002944"
     CDS             3689..4915
                     /gene="mdtG"
                     /locus_tag="ACHKUN_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO6661166.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4998..5372
                     /gene="msyB"
                     /locus_tag="ACHKUN_002945"
     CDS             4998..5372
                     /gene="msyB"
                     /locus_tag="ACHKUN_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6661167.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5373..5600)
                     /gene="yceK"
                     /locus_tag="ACHKUN_002946"
     CDS             complement(5373..5600)
                     /gene="yceK"
                     /locus_tag="ACHKUN_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6661168.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5773..8316)
                     /gene="mdoH"
                     /locus_tag="ACHKUN_002947"
     CDS             complement(5773..8316)
                     /gene="mdoH"
                     /locus_tag="ACHKUN_002947"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO6661169.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8309..9844)
                     /gene="mdoG"
                     /locus_tag="ACHKUN_002948"
     CDS             complement(8309..9844)
                     /gene="mdoG"
                     /locus_tag="ACHKUN_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO6661170.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10238..11395
                     /gene="mdoC"
                     /locus_tag="ACHKUN_002949"
     CDS             10238..11395
                     /gene="mdoC"
                     /locus_tag="ACHKUN_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO6661171.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11403..12884)
                     /gene="clsC"
                     /locus_tag="ACHKUN_002950"
     CDS             complement(11403..12884)
                     /gene="clsC"
                     /locus_tag="ACHKUN_002950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO6661172.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12826..13359)
                     /gene="ymdB"
                     /locus_tag="ACHKUN_002951"
     CDS             complement(12826..13359)
                     /gene="ymdB"
                     /locus_tag="ACHKUN_002951"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO6661173.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13454..13765)
                     /gene="ymdA"
                     /locus_tag="ACHKUN_002952"
     CDS             complement(13454..13765)
                     /gene="ymdA"
                     /locus_tag="ACHKUN_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO6661174.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13886..14218)
                     /gene="csgC"
                     /locus_tag="ACHKUN_002953"
     CDS             complement(13886..14218)
                     /gene="csgC"
                     /locus_tag="ACHKUN_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO6661175.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14277..14732)
                     /gene="csgA"
                     /locus_tag="ACHKUN_002954"
     CDS             complement(14277..14732)
                     /gene="csgA"
                     /locus_tag="ACHKUN_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO6661176.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14773..15228)
                     /gene="csgB"
                     /locus_tag="ACHKUN_002955"
     CDS             complement(14773..15228)
                     /gene="csgB"
                     /locus_tag="ACHKUN_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO6661177.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3821 a         3947 c         3661 g         3948 t
ORIGIN      
        1 gaagattctt tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg
       61 ttatttttgt gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta
      121 ttggatgcac ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc
      181 ttccattggc taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc
      241 acgctcagtt attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt
      301 attttgttaa tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg
      361 ggtccgctgc cgagttattc gcccatgact cgtcttgccg caaaggctgg acatctcgca
      421 ttgtacctat tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt
      481 aaaccgatca gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca
      541 caggcagact ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtcc
      601 gttatgcacg gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag
      661 cgcatgctgg gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt
      721 ggtttaacct tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa
      781 attgacaaag aaggtcagca cgcctttgtt aacttccgca tccagcatct tggctatagc
      841 tggttatatg gcacctttaa agatttcgac ggtactttta cctttgacga aaaaaatccg
      901 gctgccgata aagtgaatgt gacaattaac accaccagcg tcgatactaa tcacgccgaa
      961 cgcgataaac atcttcgcag tgcagatttc ctcaataccg caaaatatcc acaggcaaca
     1021 ttcacctcca ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg
     1081 ctgaatggcg taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac
     1141 ccatggggtg gtaaacgtgc aggcttcgag gccgaaggca aaattaagct caaggacttc
     1201 aatatcaaga cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa
     1261 ggcgtacagc agaagtaagc gtgaaatgtt gccggatggc acgcatccgg caatgatatt
     1321 tattccgttg gatcaggaat gcccagtgtt gtattcagac gtccacgaga cttattaaag
     1381 atcttattgc cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg
     1441 ctttcttcgc agcaaatctc actacaacaa cctttgtatt tttccgcaca tactggacac
     1501 tgaataaaca gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg
     1561 ccgcactggt ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca
     1621 aaaacaaaat ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg
     1681 tattcaataa ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag
     1741 gcactggcct tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta
     1801 tgtgcctgca tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt
     1861 tcgagcgcgt tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat
     1921 agtgcatcgg gatcgtcaag catggcgttc acttccgccg cttgcagata ctcgccaaca
     1981 ttgctggcat caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc
     2041 atgcgcagta cccagaaaga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc
     2101 tctaaagccg ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt
     2161 acgctgattt gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc
     2221 gcggtaaaca gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac
     2281 ttgtaaaacg aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttagcgcg
     2341 tcgttggaaa tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag
     2401 taaaaatccg cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta
     2461 catttgcgca ttaaaaatta tttgttattt acaagtgcgg caatttcgcc cagtcttcag
     2521 ccacaaattt tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgaataggtt
     2581 gaaaaacagg attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg
     2641 ttattggtta acctggttgg gtattggcgt actttggtta gtcgtgcaat tgccctaccc
     2701 ggttatctac cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg
     2761 cgcaaaaatt gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg
     2821 ccgtaaaatg gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggcat
     2881 ggcgtggttc tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca
     2941 cattcgtgac gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac
     3001 actggagctg ggtgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc
     3061 gaacgataat ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc
     3121 gatgctcgac cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt
     3181 ctggtacgca ccggatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc
     3241 cgttgagcag gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg
     3301 tctggtgccc ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct
     3361 gccgccagag tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa
     3421 agtggtcgaa aaatgcatca tgatggcacc acagcagtat atgtggttac accgtcgctt
     3481 taaaacacgc ccggaaggcg ttccttcacg ctattaaatc tcccctgccg gatgcttcag
     3541 aatggcatcc ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat
     3601 tgcgccccct ggaagtcgag cgcataatta gtgtgcttat cttttcttct tatgttcacc
     3661 gcgcctggcg caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa
     3721 ctggaaacga aacctgatcg tcgcctggct aggctgtttt cttaccggtg ccgccttcag
     3781 tctggtaatg cccttcttac ccctctacgt tgagcagctt ggcgttaccg gtcactccgc
     3841 cctgaatatg tggtccggta ttgtcttcag cattacattt ttattttcgg ccatcgcctc
     3901 accgttttgg ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat tacgctctgc
     3961 cctcggcatg ggcatcgtga tggtgttgat gggactggca caaaatatct ggcagttttt
     4021 gatcctacgg gcgcttcttg ggttacttgg cggatttgtc cccaacgcta atgctcttat
     4081 cgccacacaa gtaccgcgta ataaaagcgg ctgggcgctg ggtacgctct ccacaggcgg
     4141 cgttagtggt gcgttgctcg gcccaatggc tggcggcctg ctcgccgata gctacggctt
     4201 acgtccggta ttctttatta ccgccagtgt gctcatactc tgctttttcg tcaccctgtt
     4261 ctgcatcaga gaaaaattcc agccggtcag caaaaaagag atgctgcaca tgcgggaagt
     4321 ggtgacatca cttaaaaacc cgaaactggt actcagcctg tttgtcacca cgttaatcat
     4381 ccaggtggcg acaggctcaa ttgcccccat tctgacgctg tatgtccgcg aactggcggg
     4441 taacgtcagt aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag gcgtggcggc
     4501 tctgctaagc gcaccacgac tcggcaaact tggcgatcga atcggacccg aaaagatcct
     4561 gattacagcg ctgatctttt ctgtactgct gttgatccca atgtcttacg ttcagacgcc
     4621 attgcaactt gggattttac gttttttgct cggtgccgcc gatggtgcac tactccccgc
     4681 cgtacagaca ctgttggttt acaactcgag caaccagatc gccgggcgta tcttcagcta
     4741 taaccaatcg tttcgtgata ttggcaacgt taccggacca ttgatgggag cagcgatttc
     4801 agcgaactac ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt tattcaacgc
     4861 agtctattca tggaacagtc tacgtcgtcg tcgaataccc caggtatcga actgattttt
     4921 cgcctttcat acttgcaaaa gcggagaatc agctatcctt ttccctgaaa cctcatcaac
     4981 tcaaagggag aatcgtgatg accatgtacg caacgcttga agaagccatt gacgctgcac
     5041 gcgaagaatt tcttgcagac aaccccggca tcgacgccga agatgcgaat gtgcaacagt
     5101 tcaatgccca aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt gagttttttg
     5161 ccgacgaagg ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc gcgcaaagtg
     5221 tttttgatgg cgactatgat gagatagaga tacgccagga gtggcaggaa gagaatacat
     5281 tacatgaatg ggacgagggg gaatttcagc ttgagccacc gctggatacc gaggaaggac
     5341 gcgcagcagc tgatgagtgg gatgaacgtt aatcactcat acgggccatg atgaatgtcg
     5401 atcggcagca gtaaagtatc aaaaaccaga gagaatggca gatcaaggat cgtgacataa
     5461 cgccaggcgg agtcacgcac atcccattgc acaccgggat aatattggtt gccatgcccc
     5521 tgccccggta tagtgcgact aataatgctg ccacagccac tcagcaaggt caccatgatg
     5581 ctcaccacaa ttaaacgcat ttgatacctc tttcgtaaaa aaataccggc ataacgccgg
     5641 tatttttatt ggctcgttgt ttttgccgga tgcggcgtga acgccttatc cgacccacaa
     5701 aatcttgcaa aatcaataaa ttgcaggaac gatgtaggcc tgataagcgt agcgcatcag
     5761 gcaactacgt ttttattgcg aagccgcatc cggtttacgc aatgccagtg gattgagctt
     5821 tatcccttcg taataactca cccatgaaga atatctctcc ggggaattcc agacgcggaa
     5881 atgcagacgg gccatcgtca ccggatcgct tagcagcacc aggcgacgat cgcgattcag
     5941 cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga tttccagcac
     6001 cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa atgacgggtt
     6061 aaacactgcg tgcataaagc catcatcaag ggagcgttga cgattcatct caaggaaccg
     6121 atcggtatca accagcacct gcggcggcga atactcttcc gggatcagga acagtttcca
     6181 gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa acggtgacag
     6241 gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca accacgccat
     6301 accaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga acgcttcacc
     6361 ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc agccaaggaa
     6421 cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca gcacggaaaa
     6481 cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt ctttcgttcc
     6541 tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca ccatggtcga
     6601 agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga acaactgccg
     6661 tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg cagtagagag
     6721 cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg tcaggaatac
     6781 cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca tcaggttacc
     6841 gtggcaccag cggcggtcac gttttagctc atcaagcaag ttaggcggca attcttcata
     6901 agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca tcaacgccgc
     6961 ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg gcagcggagc
     7021 cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc cccagtagtg
     7081 cgactcgcca agttgccaga agtgcaaacc ggctgtaaac agtggcccat acacgcgggt
     7141 cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg gcgacgactg
     7201 aatgatcccg gcgttcgggt tggcttccat caggcgcacc aggccgcata aacaatcacc
     7261 ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc cccagcgacg
     7321 gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc ggcgatagaa
     7381 aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt gctctgcgac
     7441 gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt tggcattccc
     7501 ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt tcacgtcttc
     7561 gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt catcgccaac
     7621 tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga agcccattaa
     7681 cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga tcaggatacc
     7741 ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct gaccaaccat
     7801 atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca tataccaggt
     7861 cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga tggtaccgac
     7921 ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca gatagcgcgg
     7981 cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg tacgccacgg
     8041 gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat cgcgcccttc
     8101 gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt ccagacgcgc
     8161 ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt cggcatccag
     8221 cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc ttgcggcgat
     8281 gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg tagctccagg
     8341 tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg gttttcttgg
     8401 catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg ttatagcgca
     8461 ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg accggggtat
     8521 cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg atagtaccgt
     8581 caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc gtttgttgca
     8641 cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg atggtgtatt
     8701 taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa gcgacgatgt
     8761 tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct ttaccccact
     8821 cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg agatcttcaa
     8881 agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttc tccatcgaga
     8941 agctgctgac cgctaaatgt ttcgggttat tcaacggacg ccagatccat tcgccattac
     9001 cggcatggat agacagaccg ttagagtcgt gcaactccgg acgatagtta tttgcaggcg
     9061 acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc agtttgccga
     9121 ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc cctggcataa
     9181 ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa atggttaaac
     9241 gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga aattcttcac
     9301 ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa acctgacctg
     9361 caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg tttttatctt
     9421 tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt accgtgtctt
     9481 tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt cgtttgactg
     9541 cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc tgatggtaga
     9601 actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga ttaaactgga
     9661 tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg cttttgggcg
     9721 cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca atactgaaag
     9781 cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa cgcattttca
     9841 tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg cttatatcga
     9901 tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt atcgttcagc
     9961 acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt gcttctccta
    10021 aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc aaccgtctta
    10081 cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac cttgaagcct
    10141 gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca taacccgaca
    10201 gggataagac gaaaaatccg agattagtta accatatatg aatccagtac ccgcgcaacg
    10261 tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc cttttcatat
    10321 ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccat cattgtggct
    10381 gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg ttatatccgg
    10441 ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga aagtacgtgt
    10501 cgaacgtgta ggtatcccga tgttaacagc catcccccta ctgacattac cgcaatttat
    10561 tatgctgcaa tatgtcaaag gaaaagcgga aagttggcct gggctgtcat tgtatgacaa
    10621 atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg tgttagtagt
    10681 catgacgacg ctgtgcgtat ggatatttaa gcgcatcaga aataatttag aaaattctga
    10741 taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat gcctcggcat
    10801 cggttatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc tgagtaatgg
    10861 catgttcaat tttattgtca tgcaaacgct gttttatttg ccgttcttta tcctcggcgc
    10921 actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc gtggctgtac
    10981 ccttgcagca gcattggcgt ttgtcgctta tttactcaac cagcgctatg gcagtggcga
    11041 tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt ggatggtgaa
    11101 tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg tgacttattt
    11161 tgttaacgca tcgctgttta tctatctggt tcaccacccg ttaacgctgt ttttcggcgc
    11221 atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc tgatatttgt
    11281 agtagggatt gcgataattc tgtatgaaat tcatttgcgc atcccgttac tgaagttttt
    11341 gttctctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc gttaagccac
    11401 atttacaata accattccac gggcaatatc gacgccagtc tgaccataac ccgcttccag
    11461 aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg atcaacccag
    11521 ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg gctctgaata
    11581 aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc catttcagta
    11641 ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt tttaccatcg
    11701 atgctaaagg ttttagcatg caggctggct ccggaattac cggttatgcc gcgatcgtgt
    11761 aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc atagcggagc
    11821 aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt agcggcaaga
    11881 gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag ttgcgccaca
    11941 cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact gggtgagccc
    12001 atgatatcga acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc ctcccctttc
    12061 gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt tccatcaacc
    12121 agatgattta taaacggact ggattccatt ttgcgtaagt aacgatgcgt catagcatcg
    12181 ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg gacatccagc
    12241 acttgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc atcggcaacg
    12301 tcctctacca cgggccctat tgccataaca tctaaatccg aaaaaagtgg ctcttcccct
    12361 gccccaaaat aggcatcacc aatatttcgc ccccctacca gcgtcaccac gccatcgaca
    12421 gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat ataaccaagc
    12481 ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg gggatggctg
    12541 tcaagcaagc gtaaaatgtc gtcaagtccg ggcgtattgt tgtcgtccag caacaaacgg
    12601 acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg acccgacata
    12661 tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgccatttc ggccagacga
    12721 taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca ctgaccggga
    12781 tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt catctccttg
    12841 ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga caaagtatac
    12901 ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg cgatttcagc
    12961 cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag ccactgacgt
    13021 atagctgttt gccgccacca gtcgtaggct attgagatag gcatcctgca aaagctggtc
    13081 ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgcactacgg ctttagcggg
    13141 aagagcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct gctgttgcct
    13201 gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg ccccatcgac
    13261 gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa cggccagttt
    13321 ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt acaccttctt
    13381 gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc agcaacgtgc
    13441 gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc tgtttatcaa
    13501 gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc attaacgctt
    13561 tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt tctacgggtg
    13621 atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa tggatcaccc
    13681 ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aagcaaactg ccgagcataa
    13741 gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt catccttgag
    13801 ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct ctccagattc
    13861 atcttatgct cgatatttca acaaattaag acttttctga agagggcggc cattgttgtg
    13921 ataaatgaag tgactgtcca tcagaaacag taacaactat tttcacccga tcttccgggg
    13981 aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat gagagggtct
    14041 tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat atttgtactc
    14101 tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca tccccttgct
    14161 gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac gcattcatat
    14221 tcttctcccg aaaaaaaaca gggcttgcgc cctgtttctg taatacaaat gatgtattag
    14281 tactgatgag cggtcgcgtt gttaccaaag ccaacctgag tcacgttgac ggaggagtta
    14341 gatgcagtct ggtcaactgc agcaccgttg ccaccaccga actgtttaac agtcatttca
    14401 gaattttttc cgttccactg atcaagagtg gcgctgttac cgaagccacg ttgggtcaga
    14461 tcgattgagc tgtcatctga gccctgacct acatctgcac cattaccacc gccatgctgg
    14521 gtaatagtca agtcagagtt acgggcatca gtttgcagag caagtgcaga gttaccgcca
    14581 ccgtactggt aaatgttcag ctctgaattt gggccgctat tattaccgcc accaccgtgg
    14641 ttaccgccgc cgccgtactg aggaacaaca cctgccagag cgctaccgga gaatacgatt
    14701 gctgcaattg ctgctacttt taaaagtttc atgtaaaacc cccatcggat tgatttaaaa
    14761 gtcgaatgga aattaacgtt gtgtcacgcg aatagccatt tgcgactgtc tctgcactac
    14821 aattgccgtt ttttgagtac catactgtgt aatatttgct ttattaccag aacctttctg
    14881 gataatcatc gcagtattac cataagcacc ttgcgaaata ctggcatcgt tggcactgcc
    14941 cgcctgatca atatatgcaa ggttataatc tcctgtctgg tcaatctttg cccggttgct
    15001 actaccttct tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta actgagcact
    15061 attattcgtc ccagcttgac caattatggc tgcctgatta aatgaagact tactcaattc
    15121 atttaccgcg aagttatatt ctgaattagc taaatcataa cctgctgcgg ctgcaatccc
    15181 aggcgcaccc agtattgtta acatcataaa taacaatttg tttttcatgt tgtcaccctg
    15241 gacctggtcg tacatttaag aaattaaatc atttcaactt ggttgttaac gcaacctgta
    15301 ttttgttaac gctgcgttac gatggaaagt atgtctgcgg aaatattttt aataactcac
    15361 ccgcgcgttg tattttt
//
LOCUS       DAZFRJ010000104        15320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000104 DAZFRJ010000000
VERSION     DAZFRJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.104"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(172..1083)
                     /gene="gsiD"
                     /locus_tag="ACHKUN_002956"
     CDS             complement(172..1083)
                     /gene="gsiD"
                     /locus_tag="ACHKUN_002956"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO6661178.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1086..2006)
                     /gene="gsiC"
                     /locus_tag="ACHKUN_002957"
     CDS             complement(1086..2006)
                     /gene="gsiC"
                     /locus_tag="ACHKUN_002957"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO6661179.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2024..3562)
                     /gene="gsiB"
                     /locus_tag="ACHKUN_002958"
     CDS             complement(2024..3562)
                     /gene="gsiB"
                     /locus_tag="ACHKUN_002958"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO6661180.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3582..5453)
                     /gene="gsiA"
                     /locus_tag="ACHKUN_002959"
     CDS             complement(3582..5453)
                     /gene="gsiA"
                     /locus_tag="ACHKUN_002959"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO6661181.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5440..6405)
                     /gene="iaaA"
                     /locus_tag="ACHKUN_002960"
     CDS             complement(5440..6405)
                     /gene="iaaA"
                     /locus_tag="ACHKUN_002960"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6661182.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6609..7844
                     /gene="moeA"
                     /locus_tag="ACHKUN_002961"
     CDS             6609..7844
                     /gene="moeA"
                     /locus_tag="ACHKUN_002961"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO6661183.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRHGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            7844..8593
                     /gene="moeB"
                     /locus_tag="ACHKUN_002962"
     CDS             7844..8593
                     /gene="moeB"
                     /locus_tag="ACHKUN_002962"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO6661184.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8669..9331)
                     /gene="fsa"
                     /locus_tag="ACHKUN_002963"
     CDS             complement(8669..9331)
                     /gene="fsa"
                     /locus_tag="ACHKUN_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6661185.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9462..10361
                     /gene="ybiY"
                     /locus_tag="ACHKUN_002964"
     CDS             9462..10361
                     /gene="ybiY"
                     /locus_tag="ACHKUN_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO6661186.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10367..12799
                     /gene="ybiW"
                     /locus_tag="ACHKUN_002965"
     CDS             10367..12799
                     /gene="ybiW"
                     /locus_tag="ACHKUN_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO6661187.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12945..13760
                     /gene="ybiV"
                     /locus_tag="ACHKUN_002966"
     CDS             12945..13760
                     /gene="ybiV"
                     /locus_tag="ACHKUN_002966"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO6661188.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13912..15177
                     /gene="ybiU"
                     /locus_tag="ACHKUN_002967"
     CDS             13912..15177
                     /gene="ybiU"
                     /locus_tag="ACHKUN_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO6661189.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVISPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3701 a         4240 c         3910 g         3469 t
ORIGIN      
        1 acatttaaca aaataactat ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg
       61 ctttttaacc aaattacccg attctacaaa atcagcagaa agttacaaca cttgcttagg
      121 gcaataattt cctgtgaggt gattaccctt tcaagcaata ttcaaacgta actatccttt
      181 aattttcgga tccagcgcat cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt
      241 cagaaaaata gccagggccg gaaaaacagc gacatgcggc gcgataacca tatccgctcg
      301 tgcctcattg agcattgctc cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa
      361 tgacaggctg gcggcagaga taatcgaggt gccaatgcgc atggtgaaaa acaccacgat
      421 agacgagacg gtcccaggca agatatgacg caataaaatg gtcatatcgc tggcaccaat
      481 actgcgtgct gactcaataa aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag
      541 tcgggcaaac gcggggatgg aaaaaatggc gactgcgata atcacgttag caatgccgct
      601 tcccaacacc gcaacaacag cgatcgccag taaaataccc gggaaggcaa acagcacatc
      661 gcaaatgcgc atgatcaacc gatcccacca gccttcataa tatccagcca acaagcccag
      721 caacgtcccg atcgccgcac cgataaacac ggcaaacacg cccgccgcca gcgagatttg
      781 cgcaccaacc aggacacggc tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg
      841 ctgcaaagaa ggtccgttat tcagattgtc ataatcaaaa taattttcgg cgtcataggg
      901 agcgatccag cgtgcaaaaa tggccaccac aatcaataaa ataacgaata atgcggcggt
      961 catcgccata tgctgacggc gaaatcgtcg ccagaattca tgccacggtg tacgcacctg
     1021 gtcaggtttg accagtggca tggcgtttaa caccgcctga cgtcgccagt taaatagtcg
     1081 catccttact tgtacctgat agccgggtta atggcggcgt aaagcacatc caccactaag
     1141 ttgataagaa taaattccag cgagaaaagc agaatttccg cctgaatcac cgggtaatcg
     1201 cgcatttcta cggagtcaac gagtaagcgt ccaagtccgg gccagttgaa aactttctcc
     1261 accacgatag aaccaccgag caaaaagcca aactgtaagc ccatcatggt cactaccggg
     1321 atcatcgcgt tacgtagccc gtgtttgagg acaacccagg tttcgctcac ccctttcgcc
     1381 ctcgcggtac gcatataatc ttcgcttaaa acatcgacaa acgacgcgcg ggtaaagcgc
     1441 gccatcacgg ctgccactgc cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa
     1501 ctgtctgctc ccacggtagg cagccagccc agttcaacgg agaatacctg aattaaaagc
     1561 atccccagcg caaatgccgg aaacgagatc cccgacaccg caatggtcat actcaatcga
     1621 tccggccaac gattacgcca gacggcggcg ataattcccg ccgccatacc aaatatcacc
     1681 gcccagacca tactggttat ggtcagccac agcgttggca taaagcggct ggcaatctca
     1741 tcggcaaccg gacgacgcga caccatcgac aggccaaaat ccccctgcac agcattgctg
     1801 atatagtgcc agaactggtg atacagcggc tgatccaacc ccagctgctg acgcaccagt
     1861 tctataacct gcgcatcagc ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc
     1921 atatgcacaa ataaaaacac cagcaccgag acgataaaca gcgtcggaat caaccccagt
     1981 aagcgtttga taacgtaatt aagcattcca ctccctgcgt tgcttattgc aaatccgcgt
     2041 cttcaaagct gaagccggtg tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg
     2101 ccgacaccag tttttctacc accagcggga tccacggcga ctcttgccag atgatatcct
     2161 gcgccgcctt atacaagcgg gtcttttccg ccggatcatt agttttcagt gcctgagcca
     2221 ggaagtcgtc cacctgttta ttgctgtaaa atgcggtatt aaacagcgtc ggtggccagt
     2281 tttgcgaggc gaacagtggc gatagcgccc agtccgcttc gccggttgaa gccgaccagc
     2341 cagtgtagaa catccgcacg ccgctctctt tttgcccttt accttcaact tctgccgccc
     2401 gctgtccggc atccatcgcg gtcacctggg ctttaatccc gacctgcgct aactgctgct
     2461 gggtaaattg cagcactttc tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac
     2521 tgaaaccgtt gggatatccc gcctctttca gtaattcgcg cgctttcact ggatcgtaag
     2581 gccacggttt ataactttgc gcgtaggcga tacttggcgg taccacacca gtagctggcg
     2641 ttgcatagcc cgcaaaggca actttcacca gcgccggacg gttaatggcg taattcagcg
     2701 cctcacggac cttcgggtta tcgaacggct tttgcgtcac gttcatactg atataacgct
     2761 gcataattga cggactggcc atcaactcga tatttttgtt tttctccagc agtgtggcct
     2821 gctcgtaggg aatggggaaa gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg
     2881 tgttgttatc cgccaccgga cgccaggtta tgctgtccag tttgggcaat cctggctgcc
     2941 agtaacccgc gaattttttc accttcacaa aatcggtctg attccaggta tccagttcat
     3001 acggtccggt tcccaccgga tgaaaaccaa tctccttgcc atatttttcc agcgctgccg
     3061 gtgaaatcat cgcggtcgcc ggatgggcaa gaatattaat aaacgctgag aacggctgtt
     3121 tgagggtaat ctttaccgtt gtcggatcga tcgcctccgt tttggcaata ttcttataca
     3181 ggttatagcg tttaagatga ttcgccggat cgctggcccg gtccagattc gcttttaccg
     3241 ccactgcgtt gaaatcggtg ccatcctgga atttaattcc ttcccgcaat ttcacgatgt
     3301 aagtaatgcc gtcatcggaa acggtataac tctccgccag cacgtttttc agtttcatct
     3361 ctttatccag accgaacagc ccctggtaaa acgatttcgc tacggcctga gataacgtgt
     3421 catttgcgtc atacggatcg agcgtggtga aattcgatcc taccgccacc accacatctt
     3481 tggcagcgaa tgcacaagat gccatcaacg ctgtcgcaat gcccagcgcc actaacccac
     3541 tacggtgtac agctcttgcc attttattct ccgcctgaat gttatctacg catgaatgcg
     3601 tattctgatt gtggttgtgc gacgtaatgc cccggcccga cgcattgcaa cgagacggct
     3661 gccacctctt cgccacgcag atgaatattg ctgggaagat cgtccgacag cagtacacgc
     3721 tgcggtcgtt gtcgggacgg ttcagcgacc ggaactgccg ccagtaattt acgcgtataa
     3781 ggatgctgcg ggttttcgaa gaccgcgcgc cgtggaccaa tttcaacaat ttgcccgaga
     3841 tacatcaccg ccacacgatg actaatccgc tctaccacgg ccatatcgtg ggagataaac
     3901 agatacgcaa tgccgaaatc acgctggaga tcgagcaaca agttgataat ctgcccgcga
     3961 atagaaacat ccagcgccga aacggcttcg tcggcaatga tcacttttgg attcaatgcc
     4021 aacgcgcgag caatgcagat gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc
     4081 caggcatgtt caggtaacag gcccacgcgc tccagcaacc acgcaacgcg tgcagccgcg
     4141 tctttacctg gcaataaacc gtgtacacgc agcggttcga taatcgaatc accgatggtc
     4201 tgacgtgggt ccagcgaagc gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat
     4261 gcctgaagtt tgccgggtga caaggtatcg attctctgac cgttaaagat aatttcgccg
     4321 ccctgcgatt cgaccaggcg cagcaacgcc cgcccggtag tggatttacc gctgccagac
     4381 tcgcccacca gcgatagcgt ttcgccaggc cagagatcaa aactaacttt ctcaacggca
     4441 tgcacttccc gcgttacgcg attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga
     4501 ttacgcactc gtaaaacagg ttcgccatcc accaccgttt tctgctcgat ggggggggcc
     4561 tgtttcgctg gatgttcaag cgatatcaac gggaaacgtc ggggataatc taaccctttc
     4621 atcgcaccaa gttgcggaac agcagctaac agcgcacggg tgtaaggatg ttgcggtgca
     4681 tgaaaaatct gttcgacggt acccgtttcc accgcctcgc cctgatacat caccagtacc
     4741 cgatcggcaa tctctgccac cacgcccata tcgtgagtga taaagataac gcccatcgac
     4801 atctcttttt gcaatacttt gattaattgc aggatctgcg cctgaatagt gacatccagc
     4861 gcggtggttg gctcatcggc aatcagcacc gccgggcggc atgacagcgc catcgcaatc
     4921 atcactcgct ggcgcatccc gccagagagt tgatgcggat aacgtgaaag aatggtttgt
     4981 gcctcaggaa tgcgtacctg atccagcatc cgcttcgcct cgaccatcgc ttcttcacga
     5041 ctggcgttct gatgcagacg aattgattcg gcaatctgtt cacccacagt aaataccggg
     5101 ttcagcgatg tcatcggctc ctgaaatatc atcgccatat ccgcaccgcg aacatggcgc
     5161 atttgtgcag cgctctgctc gctaagttca atcacttcgc gactgcgccg ctgcaacagc
     5221 attttatcgc actgtactaa accgcccgcc tgttccaaca ggcgcatcaa tgccaacgca
     5281 gtcactgact taccggagcc ggattcgcca acaattgcca gcgtctcacc gcgttgcaga
     5341 ctaaaagaga gattgcggac cgcagctatt ttctgctggt cctgcataaa ggcaatattc
     5401 agattttcaa ccgccagcac attaccggca tcaagttcat cactgtgtgg caacggtgtc
     5461 ccctttttca cggtagatac cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata
     5521 cattccttcg gtgttaaacg gtagcgcgac attcccttca tggtcgatag cgattaagcc
     5581 accgctaccg ccaagcgcag ggagtttttc cattactacc cgctcgcagg cttccgcgag
     5641 acttaatccg ccgtaatcca ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa
     5701 gacttcgccc gtgccggtac aagaaaccgc cacactggcg ttattggcgt agcatccggc
     5761 acccactaag ggactatcgc caactcgtcc gggtaattta ttggtcattc cgcctgtgga
     5821 cgtggctgcc gccaaattgc cgtctaaatc caacgccacg gcccccacgg tgcccatttt
     5881 ctgtttttca tccagtggcg caccgctatg gtcgaggacg gttgcccctt ccttgcgcgc
     5941 tgccagtagt tgttcataac gcaaaggcgt ggagaaaatc tccggcgaga cgcgctccat
     6001 gccacgagca aacgcaaaat tttctgcccc ttcgccaatc atcatcacat gcgggctttg
     6061 ctccatcacc agccgggcgg caagaaccgg attacgcaga tgactaacgc ccgccaccgc
     6121 accggctttc agggtgttac catccatcac acaggcgtcc agttcatggg tttcatcacg
     6181 cgtaaagaca gcgccaattc cggcgttaaa cagtggacac tcttccagca gacgcaccgc
     6241 ttccgtcacc acatccagcg cactttcgcc cgcttccaac attttctgcc cggtttcaac
     6301 aatggcagac aacgcctcaa tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct
     6361 aattgcacct gcgccaccat gaattgcaat gactgctttg cccatagtat tcgtccatta
     6421 agggtggctc gccacttttg ctataaatat cagaattttt atgaattact cattcaattt
     6481 ttgaatatag aaagatggat atggcatgta aaggcaggag ccaacgctac gcccgaggtg
     6541 gcatacagtc tgtggcgttt ttctgacata ataggcaaat tcgattttgc ctccgcagga
     6601 gtgttttcat ggaatttacc accggattga tgtcgctcga caccgcgctt aatgagatgc
     6661 tttctcgcgt caccccactg accgcccagg aaacgctgcc actggtacag tgttttggtc
     6721 gtattctggc gagcgatgtc gtttcgccgc ttgatgttcc ggggtttgat aactccgcaa
     6781 tggacggcta cgcggtgcgt ttagccgata ttgcctccag gcaaccgctg cccgttgccg
     6841 gtaaatcctt tgccggtcag ccgtatcatg gtgaatggcc tgcgggcacc tgcattcgta
     6901 ttatgaccgg tgcgccggtg ccggaaggct gcgaagcggt ggtgatgcag gagcagactg
     6961 aacaaacgga caatggcgtg cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc
     7021 gtcacggtga agatatctct gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta
     7081 ccgcagagct gccagtaatt gcttcactgg ggattgccga agttccggtg attcgtaaag
     7141 tgcgtgtagc gcttttttct accggtgatg aactccagtt gcccggtcag ccgctgggcg
     7201 acggccaaat ctacgacacc aaccgtctcg ccgtacacct gatgttagaa cagctgggtt
     7261 gcgaggtaat caacttaggg attatccgcg acgatcccca tgccctgcgc gccgcattta
     7321 ttgaagccga cagccaggcg gatgtagtga tcagttccgg cggtgtttca gtgggtgaag
     7381 cggattacac caaaacgatt cttgaagagc tgggggagat cgccttctgg aagctggcga
     7441 ttaaaccagg taaaccgttc gcgttcggta aactcagcaa tagctggttc tgcggcctgc
     7501 cgggcaaccc ggtttcagcg acgctgacct tctatcaact ggtacagcct ttgctggcaa
     7561 aactaagcgg caacaccgcc agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc
     7621 gcctgaaaaa aacgccagga cgtcttgatt tccagcgcgg cgtgctgcaa cgcaacgccg
     7681 acggtgaact tgaagtgacg accaccggac atcagggttc acatatattt agctccttta
     7741 gtctcggcaa ctgctttatc gtgctggaac gcgatcgcgg caatgtggaa gtgggcgaat
     7801 gggtggaagt agaaccgttt aacgcgttgt tcggaggcct gtaatgacgg aactcagcga
     7861 tcaggagatg ctgcgctaca accggcaaat cattctgcgc ggttttgatt ttgacggtca
     7921 ggaggcgcta aaagattctc gtgtgctggt agtcggcctc ggtggactcg gctgcgcagc
     7981 ttcgcagtat ctggcaagcg ccggtgtcgg caacctgacg ctgctcgact tcgactcggt
     8041 ttcgctctcg aatctgcaac gccagacgct gcacagtgat gctacggtcg ggcaaccgaa
     8101 ggtagaatcc gcccgtgacg ccatgacgcg gatcaaccca catatcgcga ttacgccagt
     8161 caatgcactg cttgatgacg ctgaacttgc tgcgatgatt gcaaaacacg atctggtact
     8221 cgactgtacg gataacgttg cggtgcgtaa tcaactgaac gctggctgtt ttgccgcgaa
     8281 ggtgccgctg gtttccggcg cggcaattcg tatggaaggg caaatcacgg tcttcactta
     8341 tcaggacggc gaaccgtgct atcgctgcct tagccgtttg tttggcgaga acgcattaac
     8401 ctgcgtagaa gcaggcgtga tggcaccgtt gatcggcgta attggttcgt tgcaggcaat
     8461 ggaagcgatc aaactgctgg caggttatgg caaaccggcc agcgggaaaa tcgtcatgta
     8521 cgatgcaatg acctgtcagt ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt
     8581 gtgcgggcag taattgccac ctccaggcca gataagacgc ggcactccgc cacgtcatca
     8641 gcaatttatg aggatgcaga acgtccggtt aaatcgacgt tctgccaaac gctcccagcc
     8701 agtcctgttc aaacttcgcc acagcggctt ccaccgccgg atagctaatc atctgttgtg
     8761 ccacatccag tggcagcgta attgattcac atcctgccag taagcagtcc agcgcctgac
     8821 gcggggtttt gaaactcgct gccagcactt tcgcctgcgg cgcatgcatt ttcaataact
     8881 ggtgtaagtc ggtcacagtc tgaatgccgc taccaccctg agcatcaata cgattaacgt
     8941 aaggcgcaac atattccgca cctgccagcg ccgacagcag cccttgtgct gcgccatata
     9001 ccgcggttcc cagcgttgga atcccttccg cttttaacat cttaatagct gccagcccct
     9061 cggcggtcac cggaactttc accacgatat ccgcaataat agaacgcaat ttacgtgcgt
     9121 cattcaccat cccttcagca gtggtagcca ttacctgggc aaacagacgc ccctgaccgc
     9181 ccatcgcttc atgaagttgc ggaagcacaa catccagcgg tttgttaccc gcggcgataa
     9241 tgcttgggtt agtggtcaca cccgccagcg gaaaaatacg tgacagcgcc ttcaccgcaa
     9301 caacgtctga agtatccaga tacagttcca taaccatcct caaaatgtac tttttgtctg
     9361 aatgtagact atcacgggaa aactctctga acagttgacg cacatcaatc taactttcat
     9421 tcgaaagcat tttaatgttt gaacgaaaca gagaggcaac tatgattttc aatattcagc
     9481 gctactcgac ccatgatggc cccggtatcc gcacggtcgt atttcttaaa ggctgttcgc
     9541 tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg cacgcaggat ctgctgtatg
     9601 acgcacgact gtgtctggaa ggctgcgagc tgtgcgctaa ggccgcgccg gaagtgattg
     9661 agcgcgcgct gaatggtttg cttattcatc gggaaaagtt aaccccggag catctgacgg
     9721 cgttaaccga ctgctgtccg acacaggcat taaccgtgtg tggtgaagtg aaaagcgttg
     9781 aggagatcat gacgaccgtt ctgcgcgata aaccgtttta tgatcgcagc ggcggcggtt
     9841 taacgctttc gggtggtgag ccctttatgc agccggaaat ggcgatggcg ctactgcaag
     9901 ccagccacga ggcaggcatt catactgcgg tagaaacctg tctgcatgtg ccgtggaaat
     9961 atatcgcccc ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa cacgttgccg
    10021 acgcgccgtt taaacagtgg accgacggta acgccgccag agtgctggat aacctgaaaa
    10081 aactcgccgc agcgggcaaa aaaatcatta tccgcgtgcc gctgattcag ggctttaatg
    10141 ccgacgaaac ctctgtaaaa gccattaccg attttgccgc cgacgagctg cacgttggcg
    10201 aaattcattt tctgccctac cacacgctgg gcatcaacaa atatcactta cttaatctgc
    10261 cctatgacgc cccggaaaaa ccgcttgatg cgccagaact gctcgacttt gcccagcagt
    10321 atgcctgcca gaaagggtta accgcgacct tacgaggata acaaccatga ccacactgaa
    10381 actggacacg ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa
    10441 accgccagtc tgtaccgagc gcgcgcagca ctataccgag atgtatcaac aacatctcga
    10501 taagccgatc ccggtacgtc gcgcgctggc actggcgcat cacctggcga atcgcaccat
    10561 ctggatcaaa cacgatgagt tgatcattgg caaccaggca agcgaagttc gcgccgcgcc
    10621 gatcttcccg gaatatactg tctcgtggat cgaaaaagag attgatgatc tggcagatcg
    10681 tcccggtgct ggctttgcgg tgagcgaaga gaacaaacgc gttctgcatg aagtgtgccc
    10741 gtggtggcgc ggtcagaccg tacaggatcg ctgctacggc atgtttaccg atgagcaaaa
    10801 aggtctgctg gcgaccggaa tcattaaagc ggaaggcaat atgacctccg gcgatgcgca
    10861 cctggcggtg aatttcccgc tgctgctgga aaaaggtctt gatggtctgc gtgagaaagt
    10921 agcggaacgt cgctcgcgca tcaatctgac ggtgctggaa gatttacatg gcgaacaatt
    10981 cctgaaagcg attgatatcg tgctggtggc agtcagtgaa cacattgaac gtttcgctgc
    11041 cctggcgcgt gaaatggctg ctaccgaaac tcgcgaaagc cgtcgcgatg aactgctgac
    11101 gatggcagaa aactgcgatc ttatcgccca ccagccgccg cagactttct ggcaggcgct
    11161 gcaactgtgt tacttcatcc aattgatttt gcagatcgaa tctaacggtc actcggtatc
    11221 gtttggtcgt atggaccagt atctctaccc gtactatcgc cgcgacgttg aactcaacca
    11281 gacgctggat cgcgaacacg ccatcgagat gctgcatagc tgctggctga aactgctgga
    11341 agtgaacaag atccgctccg gctcacactc aaaagcctct gcgggaagtc cgctgtatca
    11401 gaacgttacc attggcggac aaaatctggt tgatggtcaa ccaatggatg cagtgaatcc
    11461 cctctcttac gcgatcctcg aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag
    11521 cgtgcgttac catgcaggaa tgagtaatga tttcctcgac gcctgcgtac aggtgatccg
    11581 ttgcggcttc gggatgccgg cgttcaacaa cgacgaaatc gtgatcccgg aatttattaa
    11641 actcggtatt gaaccgcagg acgcttacga ctacgcagcg attggttgta tcgaaaccgc
    11701 cgtcggtggc aaatggggct atcgctgtac cggcatgagc tttatcaact tcgcccgcgt
    11761 gatgctggcg gcgctggaag gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca
    11821 agaaaaagcg ttgtcggcag gcaactttaa caatttcgat gaagtaatgg acgcgtggga
    11881 tacgcaaatc cgttattaca cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat
    11941 gctggaagag aacgtgcacg atattctctg ctcggcgctg gtggatgact gtattgagcg
    12001 agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg gtttctggcc tgcaggttgg
    12061 cattgccaac ctcggcaaca gcctggcggc agtgaagaaa ctggtatttg aacagggcgc
    12121 gattggtcag caacagctgg ctgccgcact ggcggatgac ttcgatggcc tgactcatga
    12181 gcaactgcgt cagcggctga ttaacggtgc gccgaagtac ggcaacgacg atgatactgt
    12241 cgatacgctg ctggctcgcg cttatcagac ctatatcgac gaactgaaac agtaccataa
    12301 tccgcgctac ggtcgtggtc cggttggcgg caactattac gcgggtacgt catctatctc
    12361 cgctaacgta ccgtttggcg cgcaaactat ggcaacgccg gacgggcgta aagcccacac
    12421 cccgctggca gaaggcgcta gtccggcctc tggtactgac catctcggcc ctactgcggt
    12481 cattggctcg gtgggtaaac tgcctacggc agcgattctc ggcggcgtgt tgctcaacca
    12541 gaaactgaat ccggcaacgc tggagaacga atctgacaag cagaagctga tgatcctgct
    12601 gcgcaccttc ttcgaggtgc ataaaggctg gcatattcag tacaacatcg tttcccgcga
    12661 aacgctgctg gaggcgaaaa aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc
    12721 gggttattcc gccttcttca ccgcgctctc tccagacgct caggacgata tcatcgctcg
    12781 tactgaacat atgctgtaat ctcttcggcc cggctgcggc gtcgccgggc taaatcattc
    12841 acatcatcaa tttcatcctt actttcattc gaaatataat ttgtgctctg cgtcacattg
    12901 ttattacact gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta
    12961 tcgtcacaga catggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt
    13021 ttatggcgca atatcaggaa ctgaaaaagc gcggcattga gttcgttgtc gccagcggta
    13081 atcagtatta ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg
    13141 cggaaaacgg cgcactggtt tacgaacatg gcaagcaact gttccacggc gaactgaccc
    13201 gacatgaatc gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg
    13261 cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga
    13321 tggcaaaaca ctaccatcgc ctgaaagctg taaaagatta tcaggagatt gacgacgtac
    13381 tgttcaagtt ttcgctcaac ctgccagatg aacaaatccc gttagtgatc gacaaactgc
    13441 acatagcgct cgatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacctga
    13501 ttattcccgg tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt
    13561 caccgcaaaa tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg
    13621 cgcgttattc ctttgcgatg ggcaatgctg cggaaaacat taaacaaatc gcccgttacg
    13681 ctaccgatga taataatcat gaaggcgcgc tgaatgtgat tcaggcggtg ctggataaca
    13741 catccccttt taacagctga cctctctacc ggagccgtgc gctccggttt tcccttcttt
    13801 ttacatcctg tacatcacat caaatcatta aacttgcatt aacttattta taacttatat
    13861 tgtcacatgc aaatcagaat ggtttcgaat gaaagttaat gagaggtggt tatggcttct
    13921 acttttacca gcgacacatt gcctgccgat cacaaagcag ctatccgtca gatgaagcac
    13981 gcgctgcggg cgcagcttgg cgacgtccag cagatcttta atcagctaag cgatgacatt
    14041 gccacgcgag tggctgaaat caacgcactc aaagcacagg gcgatgccgt ctggccggtg
    14101 ctgtcttatg ccgatatcaa agcaggtcat gttactgcag agcagcgcga acagattaaa
    14161 cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg aacaagcgct aggctgggat
    14221 cagtcgatgc tggactatct ggaccgcaac cgctttgacg aggtctacaa aggccccggc
    14281 gataatttct tcgggacgct cagcgcttca cgtcccgaga tttaccccat ctactggtcg
    14341 caggcgcaaa tgcaggcccg ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat
    14401 cgtctgtgga catttgaaag tgatggaaag caatggttta acccggatgt gagcgtcatc
    14461 tcccctgacc gtatccgccg ccgtccgccc ggaacgacct ccaaaggtct tggagcgcat
    14521 accgactccg gggcgctgga acgctggctg cttccagcgt atcagcgcgt tttcgccaac
    14581 gtctttaatg gcaatctggc gcaatatgat ccctggcatg cggcacatcg tacggaagtt
    14641 gaagagtaca cggtggacaa caccaccaaa tgttccgtgt ttcggacatt ccagggctgg
    14701 acagcgctct ctgatatgct gcctggtcag gggctgctgc acgttgtgcc cattcctgaa
    14761 gccatggcgt acgtactgtt acgtccgctg cttgatgatg tgccggagga tgaactgtgc
    14821 ggcgtagcgc ccggaagagt gttgccggta tcagagcaat ggcatccact gttaattgag
    14881 gcgttaacca gcattccaaa actcgaggcc ggagactccg tctggtggca ctgcgacgtc
    14941 atccattccg ttgcccccgt tgaaaatcaa cagggttggg gcaacgtgat gtacattcct
    15001 gcggcaccga tgtgcgagaa aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa
    15061 aaaggcgcat cgccgggcga cttcccgcgc gaggactatg aaacaaactg ggaaggacgc
    15121 tttacgcttg ccgacctcaa cattcacggt aagcgagcgt tgggcatgga tgtttgagag
    15181 ttaatggaag tgaattgatg tgggattaag aaaatcgatt cgacgttact cagattgctg
    15241 acaagtgcgc tttcttaatg cctgatgcgc tacgcttatc tggcctacaa agtcctgcag
    15301 attcaatata ttacaggtac
//
LOCUS       DAZFRJ010000105        15313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000105 DAZFRJ010000000
VERSION     DAZFRJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.105"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(247..1071)
                     /gene="iclR"
                     /locus_tag="ACHKUN_002968"
     CDS             complement(247..1071)
                     /gene="iclR"
                     /locus_tag="ACHKUN_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO6661190.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1271..4954
                     /gene="metH"
                     /locus_tag="ACHKUN_002969"
     CDS             1271..4954
                     /gene="metH"
                     /locus_tag="ACHKUN_002969"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO6661191.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5174..6805
                     /locus_tag="ACHKUN_002970"
     CDS             5174..6805
                     /locus_tag="ACHKUN_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO6661192.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6896..7585)
                     /gene="pepE"
                     /locus_tag="ACHKUN_002971"
     CDS             complement(6896..7585)
                     /gene="pepE"
                     /locus_tag="ACHKUN_002971"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO6661193.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7968..9197)
                     /gene="sorE"
                     /locus_tag="ACHKUN_002972"
     CDS             complement(7968..9197)
                     /gene="sorE"
                     /locus_tag="ACHKUN_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO6661194.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9249..10072)
                     /locus_tag="ACHKUN_002973"
                     /pseudo
     CDS             complement(9249..10072)
                     /locus_tag="ACHKUN_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10083..10880)
                     /locus_tag="ACHKUN_002974"
     CDS             complement(10083..10880)
                     /locus_tag="ACHKUN_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO6661195.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(10946..11440)
                     /locus_tag="ACHKUN_002975"
     CDS             complement(10946..11440)
                     /locus_tag="ACHKUN_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO6661196.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11440..11847)
                     /locus_tag="ACHKUN_002976"
     CDS             complement(11440..11847)
                     /locus_tag="ACHKUN_002976"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO6661197.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11857..12663)
                     /locus_tag="ACHKUN_002977"
     CDS             complement(11857..12663)
                     /locus_tag="ACHKUN_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6661198.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12733..13680)
                     /locus_tag="ACHKUN_002978"
     CDS             complement(12733..13680)
                     /locus_tag="ACHKUN_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO6661199.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14028..14900
                     /gene="rluF"
                     /locus_tag="ACHKUN_002979"
     CDS             14028..14900
                     /gene="rluF"
                     /locus_tag="ACHKUN_002979"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO6661200.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14901..15173)
                     /locus_tag="ACHKUN_002980"
     CDS             complement(14901..15173)
                     /locus_tag="ACHKUN_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO6661201.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3645 a         4002 c         4019 g         3647 t
ORIGIN      
        1 acaactaaag aaatatcata aatcgctcaa tctcataatg cagccgtaaa agtttcggtg
       61 gaatgagatc ttgcgatttt gttaatgatt aagtagtttg atgtttaaaa ataatgctga
      121 gttaataaaa gtgtgtgaag tgtatgacat taagttattt tggcgtcaat gcgattaaca
      181 gacaccctta ttctattgcc actcaggtat gatgggcaga atattgcctc tgcccgccag
      241 aaaaagtcag cgcattccac cgtacgccag cgtcacttcc ttcgccgctt taatcaccat
      301 cgcgccaaac tcggtcacgc ggtcatcggt aatacgtgaa atcggtccgg aaattgaaat
      361 tgcggcaaac ggttcgcggt gctcatcgaa aatacacgct gcaaggcaac gtagccccag
      421 tgcatgttcc tcatcgtcaa atgaataacc ccgtttgcgc gtttgggcga gatcttcttt
      481 taaatgcaca ggagacacca gcgttgcgtg ggtataggca tgtaaccctt tgcggtgcag
      541 cagcttcgtc acctgttctt cgctcagttg ggctaaaaag gctttacccg caccggaagc
      601 gtgcatcggc aatttaccgc cgataggcgc ggacattcgc atcagatgcg tacactgtac
      661 ctggtcgata ataatcgctt cgtgatcgct ttgatcaagc accgccatat tgaccgtttc
      721 gccagactct tccattaaat tgcgcaggat agggtgaaca atcgctaaca aattacggct
      781 ctggagaaag ctgctgccga ccataaaggc atgtgcgccg attgcccaat gtcccagttc
      841 gccgacctga cgcacgaaac cctgctgttg catcgtggtt agcaggcggt gggtcgtgga
      901 attgggtaac ccggcttgtt gtgccagctc tgtgagtgcc acactgccat tggattcggc
      961 aatccactcc agtaatttca ggccacgcgt taaagactga acctgtccag tcgctggtgc
     1021 ggtggcaacg gcgggttttc tgccgcgttt cgcgggaatg ggtgcgacca tgacagcctc
     1081 ctttttctgt atcgtggaaa tcattttcat ttttattgtt agctaatgca atagttgctg
     1141 aactgatccg atgagttaat gttgaacaaa tctcatgttg cgtggtggtc gcttttacca
     1201 cagatgcgtt tatgccagta tggtttgttg aatttttatt aaatctgggt tgagcgtgtc
     1261 gggagcaagt gtgagcagca aagtggaaca actgcgtgcg cagttaaatg aacgtattct
     1321 ggtgctggac ggcggtatgg gcaccatgat ccagagctat cgactgaacg aagccgattt
     1381 tcgtggtgaa cgctttgccg actggccatg cgatctcaaa ggcaacaacg acctgctggt
     1441 gctcagcaaa ccggaagtga tcgccgctat ccacaacgcc tactttgaag cgggcgcgga
     1501 tatcatcgaa accaacacct tcaactccac gaccattgcg atggcggatt accagatgga
     1561 atccctgtcg gcggaaatca actttgcggc ggcgaaactg gcgcgcgcct gtgctgacga
     1621 gtggaccgcg cgcacgccgg agaaaccgcg ctacgttgcc ggtgttctcg gcccaaccaa
     1681 ccgcacggcg tctatttctc cggacgtcaa cgatccggca ttccgcaaca tcacttttga
     1741 ccagctggtg gcggcctatt gcgaatccac caaagcgctg gtggaaggtg gtgcggatct
     1801 gatcctgatt gaaaccgttt tcgacaccct taacgccaaa gcggcggttt ttgcggtgaa
     1861 aacggagttt gaagcgctgg gcgttgagct gccgattatg atctccggca ccatcaccga
     1921 cgcctccggg cgcacgctct ccgggcagac caccgaagca ttttacaact cattgcgcca
     1981 cgccgaagct ctgacctttg gcctgaactg tgcgctgggg cccgatgaac tgcgccagta
     2041 cgtgcaggag ctgtcacgga ttgcggaatg ctacgtcacc gcgcacccga acgccgggct
     2101 acccaacgcc tttggtgagt acgatctcga cgccgacacg atggcaaaac agatacgtga
     2161 atgggcgcaa gcgggttttc tcaatatcgt cggcggctgc tgtggcacca cgccacaaca
     2221 tattgcagcg atgagtcgtg cagtagaagg attagcgccg cgcaaactgc cggaaatccc
     2281 cgtagcctgc cgtttgtccg gcctggagcc gctgaacatt ggcgaagata gcctgtttgt
     2341 gaacgtgggt gaacgcacca acgtcaccgg ttccgctaag ttcaagcgcc tgatcaaaga
     2401 agagaaatac agcgaggcac tggatgtggc ccgccagcag gtggaaaacg gtgcgcagat
     2461 tatcgatatc aacatggatg aagggatgct cgacgccgaa gcggcgatgg tgcgttttct
     2521 caatctgatt gccggtgaac cggatatcgc tcgcgtgccg attatgatcg actcctcaaa
     2581 atgggatgtc attgaaaaag gtctgaagtg tatccagggc aaaggcattg ttaactctat
     2641 ctcgatgaaa gagggcgtcg acgcctttat ccatcacgcg aagctcttgc gtcgttacgg
     2701 tgcggcagtg gtggtaatgg cctttgacga acagggacag gccgataccc gcgcacggaa
     2761 aatcgagatt tgccgtcggg cgtacaaaat cctcaccgaa gaggttggct tcccgccaga
     2821 agatatcatc ttcgacccga acatcttcgc ggttgccacc ggcattgaag agcacaacaa
     2881 ctacgcgcag gactttatcg gcgcgtgtga agacatcaaa cgcgaactgc cgcacgcgct
     2941 aatttccggc ggcgtatcta acgtttcttt ctcgttccgt ggcaacgatc cggtgcgcga
     3001 agccattcac gcagtgttcc tctactacgc tattcgcaat ggcatggata tggggatcgt
     3061 caatgccggg cagctggcga tttacgacga cctgcccgct gaactgcgcg acgcggtgga
     3121 agatgtgatt cttaatcgtc gcgatgatgg cactgagcgt ttactggagc ttgccgagaa
     3181 atatcgcggc agcaaaaccg acgacaccgc taacgcccag caggcggagt ggcgctcgtg
     3241 ggaagtgaat aaacgtctgg aatactcgct ggtcaaaggc attaccgagt ttatcgagca
     3301 ggataccgaa gaagcccgcc agcaggctac gcgcccgatt gaagtgattg aaggcccgtt
     3361 gatggacggc atgaacgtgg tcggcgatct gtttggcgaa gggaaaatgt tcctgccaca
     3421 ggtggtcaaa tcggcgcgcg tcatgaaaca ggcggtggcg tatctcgaac cgtttattga
     3481 agccagcaaa gagcagggca aaaccaacgg caagatggtg atcgccaccg tgaagggcga
     3541 cgtccacgac atcggtaaaa atatcgttgg tgtggtgctg caatgtaaca actacgaaat
     3601 tgtcgatctc ggcgttatgg tgcctgcgga aaaaattctc cgtaccgcta aagaagtgaa
     3661 tgctgatctg attggacttt cggggcttat cacgccgtcg ctggacgaga tggttaacgt
     3721 ggcgaaagag atggagcgtc agggcttcac catcccgcta ttgattggag gcgcgacgac
     3781 ctcaaaagcg cacacggcgg tgaaaattga gcagaactac agcggcccga cggtgtatgt
     3841 acagaacgca tcgcgcaccg ttggtgtggt ggcggcgctg ctttccgata ctcagcgtga
     3901 tgattttgtc gctcgtaccc gcaaggagta cgaaaccgta cgtattcagc acgggcgtaa
     3961 gaaaccgcgc acaccaccgg tcacgctgga agcggcgcgc gataacgatt tcgcttttga
     4021 ctggcaggct tacacgccgc cggtggcgca ccgtctcggc gtgcaggaag ttgaagccag
     4081 catcgaaact ctgcgtaatt acatcgactg gacaccgttc tttatgacct ggtcgctggc
     4141 cgggaagtat ccgcgcattc tggaagatga agtggtgggc gttgaggcgc agcggctgtt
     4201 taaagacgcc aacgacatgc tggataaatt aagcgccgag aaaacgctga atccgcgtgg
     4261 cgtggtgggc ctgttcccgg caaaccgtgt gggcgatgac attgaaatct accgtgacga
     4321 aacgcgcacc catgtgatca acgtcagcca ccatctgcgt caacagaccg aaaaaacggg
     4381 attcgctaac tactgtctcg ctgacttcgt tgcgccgaag ctttctggta aagcagatta
     4441 catcggcgca tttgccgtga ctggcgggct ggaagaggac gcactggctg atgcctttga
     4501 agcgcagcac gatgattaca acaaaatcat ggtgaaagcg cttgccgacc gtttagccga
     4561 agcctttgcg gagtatctcc atgagcgtgt gcgtaaagtc tactggggct atgcgccgaa
     4621 cgagaacctc agcaacgaag aactgatccg cgaaaactac cagggcatcc gtccggcacc
     4681 gggctacccg gcctgcccgg aacatacgga aaaagccacc atctgggagc tgctggaagt
     4741 ggaaaaacat accggcatga aactcacaga atctttcgcc atgtggcccg gtgcatcggt
     4801 ttcgggttgg tatttcagcc acccggacag caagtactac gcggtggcgc aaattcagcg
     4861 cgatcaggtt gaagactatg cccgccgtaa aggtatgagc gtctccgatg ttgagcgctg
     4921 gctggcaccg aatctggggt atgacgcgga ctgattcaca aatctgtcac ttttccttac
     4981 aacaaacagg gcgctcaatg agtgccctgt ctctttatta atatgaaaca cttatactgg
     5041 aaacaggctg gaataatctt agccggaaaa acgaatgtca aaaggcacag taaagatgcc
     5101 agtcagcgtg tttccggtcc tttggtcatt caattgcttc aggaacatat aacgataagg
     5161 agaacctgaa ctcgtgttaa cgctgcttca cctgctttct gccgtcgccc tgctggtctg
     5221 ggggactcat attgttcgaa ccggcgtaat gcgcgtcttc ggcgcgcgtt tgcgtactgt
     5281 ccttagccgg agcgtcgaaa aaaagccgct cgccttttgc gcggggatcg gcgttaccgc
     5341 actggtgcag agcagtaatg ccaccaccat gctggtgacc tcgtttgtcg ctcaggatct
     5401 ggtagccctc gcaccggctc tggtcattgt gctgggggca gatgtcggga cggcgctaat
     5461 ggcgcgtatt ctcaccttcg acttatcctg gctgtcaccg ttacttattt ttatcggcgt
     5521 gatttttttc ctcggacgca aacagtcacg cgccgggcaa ctgggccgcg tcggtattgg
     5581 tcttgggctg attttactgg cgctggagtt gattgtgcag gccgtaacgc cgatcaccca
     5641 ggcaaacggc gttcaggtga tctttgcctc gctgaccggc gatattctgc tggatgcgct
     5701 gattggcgcg atgttcgcca ttatcagcta ctccagcctt gctgctgtac tgctgactgc
     5761 gactctgacc gccgcaggca ttatctcctt ccccgtggcg ctctgtctgg tgattggtgc
     5821 taacctcggt tccggcctgc tggcgatgct caacaacagt gccgccaatg ccgcagcccg
     5881 ccgtgtcgcg ctgggtagtc tgctgtttaa gctggtgggt agcctgatta tcctgccgtt
     5941 tgtccatttg ctggcagaga caatggggaa gctgtcattg ccaaaagcgg aactggtgat
     6001 ctatttccac gtcttctaca accttgtacg ttgcctggtc atgctgccat ttgttgaccc
     6061 gatggcacgg ttttgcaaaa cgattattcg cgatgaaccg gaactggata cccagctacg
     6121 gcctaaacat ctggatgtca gcgcgctgga tacgcccacg cttgctctgg cgaacgccgc
     6181 gcgcgaaacc ctgcgcattg gcgacgccat ggaacagatg atggaagggc tgaataaagt
     6241 gatgcacggc gagccacggc aggagaaaga gctgcgtaag ctggcagatg atatcaacgt
     6301 tctctatacc gccattaagc tgtatctggc gcggatgcca aaagaggaac tggcagaaga
     6361 agagtcgcgt cgctgggcgg agattattga aatgtcgctc aaccttgaac aggcttccga
     6421 tatcgtcgag cgcatgggca gcgaaattgc cgataaatcg ctggcagcac ggcgggcatt
     6481 ttcgcttgat gggttgaagg aactggatgc gctctatgag caattgctca gtaatttaaa
     6541 gctggcaatg tcggtgttct tctctggcga tgtcaccagc gctcgtcgtt tgcgtcgcag
     6601 caaacatcgt tttcgcattc ttaatcgccg ctattcccac gcccacgtcg atcgcctgca
     6661 tcagcaaaac gtgcagagca ttgaaaccag ttcgctacat ttaggcttac tgggagatat
     6721 gcagcgcctg aactcgctgt tttgttcggt ggcttacagt gtgctggaac agccggatga
     6781 agatgaagga cgggacgagt attaatattg tgaatcctgt aggcctgata agacgcgtca
     6841 agcgtcgcat caggcactat gaacaagatg ccggatcatg catccggcaa tttacttaaa
     6901 aacggtgacc agcttctagc ggcaccgctt cttcaccagc cttaaacaca taagtggtgt
     6961 tcgggccacc cagcgtagcg tgacctttac tcactgtgat ccagttacct tccggtagac
     7021 caataatcgt cagttctggc gcgacgacca gcagttcgcg aatacgctgc tcacgggttt
     7081 cacctttatg gccttccggc agcgcgttgg tgaagtgcgg gttgatttgc agcgggaaca
     7141 gatttagcgc atcgaaacct tgcggatcga caatcggcat atcgttggtg gtacgaatgg
     7201 ttgggcaagc aaggttagcg cctgcgctcc agccaatata cagcgcacca cgtttaacca
     7261 cgtcagtaat tggtgccagc agcccgcgct cgcggcactg tttcagcaac tggaaagtat
     7321 tcccgccccc gacaatcacg atctcagcat tttcaatcgc ggcaacggga tcgacaacgc
     7381 tatgaatacc ggtgacagat acacccagcg gagcgagaac cgcagccgtt ttcgctgtgt
     7441 aatcatccca ggtctgcgtt acgccagcga aaggaataaa taccgctgaa cggcgaccct
     7501 gcaactgttc agcaatcagc ggcagcgcat gttccagcca ggctttaccc ggcagcgtcg
     7561 agttactcaa taaaagcagt tccattactt ctccagttag taagaaatca cgggaggcat
     7621 gctaccactg cgaatttaac cacttactga catggctcac gatgtcacgt attgcaccgg
     7681 gcgctgagac tgatgacaaa cacagcattg cctgatgcgc tacgcttacc aggcctacca
     7741 gataactgca atatattgaa ttttcgagat tatgtaggcc gaataaggct ttattccgca
     7801 tccggcataa acaatgtgta ctttgccacc aatttgagcg ccccttaagg cgctgatgtt
     7861 tttttatcct tctcgtgcac attgggccgc ttcagcaatc agccacacat caggtcgcga
     7921 agttgccgcg gcgaccagcc agcgacatgc agagttaagt agcgcgatta atcatgggaa
     7981 attgcctctg catgagtgag cagatattgc tccgcctcac cggaccagat cccctgatga
     8041 cgcgccagaa tcgccgccag tgcttgatcc tgaatctgcg tgagtgacgt cagcggcagg
     8101 tatttcccgg tatacaccag ctttttgccg ccgccgacgg caggcaattc aagcgtggtt
     8161 tcgcccgcgg cattcagccc aagaatatgt gttaccactt ttgcggcctg cacttttttc
     8221 tcttcaatca gtttgaccgc cgcgcgcatg tcgtcggtat tgccgcctga cgtgcccacg
     8281 tagtgggtaa atgcataatg cacatcgtag aaattaattg gcgcgctgaa atgtttatcc
     8341 tgcggtccgg cgaagaaatt caggcaacca tctgtcgcca gcaaggaaga ggcgagagtc
     8401 accagtcctt cattaggcac aaagacgaaa atatcatcga agccgtgacc gccactcagc
     8461 gccattagcg tatcaaatgc tgcatcggcg gcattgagat aatgaatcag tgtttgcggt
     8521 tctgatggat agtgcttgcg cgcataactc aatttatcgt tgtcggtatc ggtaatgacg
     8581 agcagcgacg ggttaacggg tccatgtagc gcataatcaa tcgccaacag tcccattggt
     8641 ccggtgccgc cgaggatcag catgcgccct tgcgggcgaa tccccatcgt gtggttataa
     8701 ctaccttcct gaagatgata gttggcgttg aacgcgccaa tcacgcagga aagcggttca
     8761 accagtgagc cttcaaaata ggtttcgccg tcgtatgcca gcaggcaatc ttgttccatg
     8821 acctcgttgg gaataaccac atgcgtggct tcgccgccca cccacgggaa ggagtagccg
     8881 gggcagtccg ggcggtcggg gagttgcagg ttggcctgaa tcacataacg ctgacccggc
     8941 tggaatttgt gctgccactt tttacccacg gccagaatat cgccgcaaaa ctcgtggccg
     9001 atgatgatgg ggttggtcgc cacatcgtcg ggtacttttt tatggttttc acccagattg
     9061 gcctctttcc aggaagagag gcacaggctg tcagtgacca ccgtcgcgag gatttcatcc
     9121 tcctgcattt caggaaggtc aaaggtttcc aggcgtaaat cacgtttacc ataaagacgc
     9181 agagctgttg ttttcataaa ccacctcatg ttgagcgatg gccccggcag tcgtgccggg
     9241 gcgaagaatc aggacaggaa gcccagcgca ttaccgataa tccctaagcc aaacagggcg
     9301 aaaatcagcc ataccgggtt aatttttttg ttgagcagac gaaccataag cagcgtcaga
     9361 ccgagcgcca gcaaaccagg gcaaagttgg tcgagaatgt tctgcacggt catggtgacg
     9421 gtggagccat cggcggcatg cgtttgtgaa accaccaacg gtacgttgat tgacgtccat
     9481 ttggtgacca gcacgcccat cacaaacagt ccgagaatcg acgcgccttc ggtgagtttt
     9541 tgcagcacat tcccgcccat atcgctgacg atattcaccc ctttgcgaaa gccgagctgt
     9601 aggccatacc acttcatcgc cagacgcacc gcattgaaaa taaagaaaaa aaaagcgggc
     9661 cgaggatgtt gccagaaagt gccagagatg cgccgagcgc agcggtaatc gggcgcagcg
     9721 ttccccaaac cagtggatcg ccaacccctg ccaatggtcc catcagaccg actttgatgc
     9781 cgttaatggt accgtcatca atttccgcgc cgttggcccg cgcctcttcc atggcggcag
     9841 tgacgccgat gaccgggcca catacggctg gcgtggtatt gaagaacacc aggtgtcgcc
     9901 tgagcgccgc aacctgatcc tcttttaatg ggtaaagtcg cttgatggcg gggatcatgt
     9961 cgtagcaaaa gcccagcccg tgaatacgtt caaagttaaa ggacgcctgt tgcaggttgg
    10021 agcgcagaaa catgctcacc agatcgctgc gtgtaatttt tctctgttcc atgatggctc
    10081 cgttaatcgt caagttggtc aagggcggtg gaggtggtgg cctggggttg aggttcagct
    10141 ttacgccact gtggatttag ctggatgtag accagggcca tgatcacgcc gacgccaccg
    10201 aacgccagca gactgagatc gaggtagcca cctgcgagga aaccgaggaa aaagaaaggc
    10261 atcaaatatt tcacgcccat catacgtagc accatggcgt aaccgaccac cacgataaaa
    10321 ccgccagcaa tttgcagacc gcgcgtcacg aattccggta tcgcgctcag catattgctc
    10381 accatatcgg cgctgacgaa cagtgagaca atcagtgccg gaatagcaac gcgcagcgcc
    10441 tgcacgccaa gcgcggagac atgcagaata tcgagagtgc gaaaccgcgc ttcttccgct
    10501 gctttatccg ccgcgtgctg gaacactacg gtgatggtac gggcaaacac agtcagcacc
    10561 tggcctgccg cagccacagg caacgcgatg gcgattccgg tggcgatgct ttgctgacca
    10621 acgataacca gaatggcgga gattatgctg gcgagtgcag aatccggaga ttgcgccgcg
    10681 ccgacgttca tccagccgag agctatcaat tccagcgtac caccgagtat tattccggtt
    10741 tttaaatcac cgagaattaa accaatcacc gtacaggcaa ttaacgggcg atgagtctga
    10801 aattcatcca gcacgctgcc cattccggca atacaggaaa aaagaaatat agcaatgatt
    10861 tgtagggtac ttatttccat gatttttcac cttttaagtg tcgtcatccc tgcctgttaa
    10921 agcatatgta cacaggcgct attatttaat ttgcgattaa ctgttcgttt attttgtcga
    10981 taatattgat tgagggatct gacgctacga cgcgtaaatc cagtatgacg ccaagattat
    11041 ttaactcatg aaatgcgtta atatcatcat catctaatga aaccgctttg gttaactgtt
    11101 ttttaccggg ccgccaggcc atgccgccaa tattcagggt gtctattttt accccctggc
    11161 gaaccatcgc taaggcatcc tgtgggcgag tgaaaagata aaaaacggtt tcgtcctgat
    11221 attgcgggtt gtgataaaca gcgacggctt tttcaatatt gaccacgtta accttcattc
    11281 ccggtggcgc agcctgacgc aataatgtcc ggcgaacttc atcgttataa acttcgtcat
    11341 tgcagataat aatgcgctgg gcgttagcca ctttcgacca gacggtagtg acctgaccgt
    11401 ggatcaggcg gtcatcaatg cgggcgagcg taatgttcat cagaaatcct cctctgccgt
    11461 tgccagttgt ttccagacaa cgcaggtttg ctttgcgatt tgcgtcaggt gttcacacag
    11521 ttcatcaaca ttcatgctgt cctggttatc aaccagttcg agcgccagcg gcagagaaag
    11581 gccgctgatt acccgcagac gaggattgcg catcgcaagc atcgctgccg cattccacgg
    11641 gctgccgcac tgtaaatcca cggcaatcag ccactcatct tgattgtgaa tgcttactaa
    11701 cttttccagc tttgctacga tgtctccggc gttctcgccg ggcacaaatt caacggcttc
    11761 aacattggcg ttgccgtaaa ccatctgaac cgattccagc atggcgcaag ctaatttgcc
    11821 gtgggcacaa aaaatggcgt tgaccataaa gcctccttag ccgcgcgttt tgccgcccgc
    11881 aatgttagtg gttactccgg tgatgtagct ggcgcgttca gacaacagat aacaaacaaa
    11941 atcagcgact tctgctaatc ttccggcgcg cccaatagga atggcgtttt tggtatagcc
    12001 ttcacgcagc tgctcgacgg tgatattgcg cgtccacgcc agcgcctctt catattccgg
    12061 cgtgcgtagc ccggtttttt ccagaatccc gggcgcgata ccgaccacac ggataccgtg
    12121 tttacccagc tctttcgacc aggagcgcgt gaagctatta agcgcggctt tggtagcggc
    12181 gtaacagctt tggccttctg agccttccag tccactttct gaggaaacat tcacaatcac
    12241 gccatcatgt tgtttgacca tctgtcgcgc caccgcctgc gacatcagaa aaacgccttt
    12301 ctggttgata ttgaccattt tttcgaatgc agcttcgttg agttcatact gcccggcagg
    12361 cgctttctca tcgaccagca gacgcgggaa attgaccccg gcgttattga ccagaccgtc
    12421 gatgcgacca aaacgctgga taatttccgc taccgtatga tttacctctt tggcgctgga
    12481 aatatcggtc ggccaaaact gatagccttt atgaccttca tattgaccat cgccaccgtg
    12541 aatatctacc atctgtacgt ttgcgccttg tgctaataat tcctctacaa tcgccagacc
    12601 aataccggat gcgccgccgg tgacgataat gattttatcc tgcaaattta accacgtttg
    12661 cataacagac tcctgatttt agataatagg gacccgccgt aatttttatt acggtaaaca
    12721 tcgggaagat ttttatttca gtaatagttc agctgtgctg ctattcgtta ccagacaatt
    12781 aatatatttt ccacgcagtg caccaataat tccgctgtat ttttcttcac tcatggcaat
    12841 gccaatggaa tatcgcgcct gctttaattt attcatttcg atagagagcg ttttttcgct
    12901 catattcgtt tcaaccattg cgccgtgaat atcaaaaaag cgcgagcaaa tatcgccagc
    12961 aacctggcgg gcattcaggt cgtcactctc ttcaccacca taaaacgcat gccagttagc
    13021 gccgtcgcga atggccggtg agccaattcc caccagggcg acatccagat tatcccagta
    13081 ggcagagatg gttttaaagt gctgagactg catgatccca ttacgaatta atgggttatc
    13141 cagcagagcc ggaaaatccg cgagatgcga ttcccctttc agcttcgctg ccgcgctgta
    13201 ggttaatgtg tttacatgat agcggctttc gagtttaccg gacggtccgc caataattgg
    13261 tacgcagatt aactgccgcg attgccccgc ctgcggcaag ttttcaacca gtgcgctcac
    13321 cgcgcgaccc caggaaaaac cgacaatatc gccaggctcc agcaagcgat ccagcagttg
    13381 cgcgccgtgt aaccccatca tcgccagttg agtctcttca tcctcatcat ttcccgacac
    13441 caccacaacg tctttcaggc caaacttttg cttcagttgc tgctccagcc agagattttc
    13501 gttgtagtca tagttgatgg cgatggtgac aattccctga tcgcggcctc gtttaagcaa
    13561 gcggctgatg gtggtgcggt aaatacgcag ttcgcgcgcg atttgcgcct gcgtcatatc
    13621 ctgttcgtaa tagagttggg caatcttcac aatcaaacgg atatcgtcac tgttttccat
    13681 tgccgcctcc tggttctgca catttgtgca aatacgcttt ctcaccgctt gatctgatat
    13741 aaccccatca tgagggcctt tcaaagcgat ttttcactct ttgcatgggg agaaatagcg
    13801 ctttctcttg cacatttgtt tctctgcgta gtagtcatca gcacaggtgt gcagcgattc
    13861 gtaatatgtt gattatcctt attttgccag gcaaatttgc atcgtacttc cggagacact
    13921 tttgtgataa aaggcgtggc agatcacatt taatttcgac gttgccgcat aaccctgttt
    13981 ttccccgcta agaggtacgg tatattccgc gttttacagg agaatttatg ctgcccgact
    14041 catcagtccg tttaaataaa tacatcagcg aaagcggaat ttgctcacgc cgcgaagcgg
    14101 atcgctatat cgagcaaggc aatgtgttcc ttaatggcaa gcgagccacc attggcgatc
    14161 aggtgaaacc cggcgacatt gtgaaagtaa acggtcagtt gattgagcct cgggaagccg
    14221 aagatttggt acttatcgcc ctgaacaagc ccgtcggtat tgtcagcacc accgaagatg
    14281 gcgagcgcga taacattgtc gatttcgtta accacagcaa acgcgtgttc ccgattggcc
    14341 gcctggataa agactcccag gggctgattt tcctcaccaa tcacggcgat ctggtgaata
    14401 agatcctccg tgcgggtaac gatcatgaga aagagtatct ggtgacggtc gataaaccga
    14461 ttaccgatga atttattcgt ggtatggggg cgggggtgcc gatcctcggg acggtgacga
    14521 aaaagtgcaa agtcaaaaaa gaagcgccgt ttgtcttccg cattaccctg gtgcaggggc
    14581 tgaaccgtca gatccggcgc atgtgcgagc atttcggcta tgaagtgaaa aagctggaac
    14641 gtacgcgcat tatgaacgtc agtttaagcg gcattccgct gggtgagtgg cgcgatttaa
    14701 ctgacgatga gttaatcgac ctgtttaaac tcattgaaaa ttcctcttcc gaggtaaaac
    14761 ctaaagcgaa ggctaaaccg aaaacagcgg gcatcaaacg tccagtcgtt aagatggaaa
    14821 aaacggcgga aaaaggcggt cgcccggcgt ccaacggtaa gcgttttacc tcgccggggc
    14881 gtaaaaagaa gggacgctaa tcagcgcctg ccgcgcgtcg acgtatttgc tgaccagcta
    14941 aatgacgctt caggatccgg tttataagcc tgctttttct tcaactggcg ggcttttttc
    15001 gcttcagcct cacgctcgac cattaattta tcgatgtact ctttcttaat gctgttggtt
    15061 tcggagttgg tcagtacgcg accatgcgca atccgcgccc gatccagcag tgttttaagt
    15121 tcacgctgct cgcgttcagt catctctttt tgggtaatac gggggagtgc catgatggtg
    15181 ccctcagtga gccagagagt tcagtgtacg ggaattcgtt tgcgagctga actgataata
    15241 ttcctttgtt ctcttcttaa atatttatct ttcttgataa ttgacgataa atacagatac
    15301 aaatttagcg aaa
//
LOCUS       DAZFRJ010000106        15300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000106 DAZFRJ010000000
VERSION     DAZFRJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.107"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1222)
                     /locus_tag="ACHKUN_002981"
                     /pseudo
     CDS             complement(1..1222)
                     /locus_tag="ACHKUN_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            1205..1903
                     /gene="tagH"
                     /locus_tag="ACHKUN_002982"
     CDS             1205..1903
                     /gene="tagH"
                     /locus_tag="ACHKUN_002982"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO6661202.1"
                     /translation="MSDPASNGGINTPFMDLPPIYASPGDRNDDVSAAEMAQRHLAVT
                     PLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLDLQQQRNSLSDKHLRPLEDNP
                     LRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVRHHEEANRAAIVESLRVLLDA
                     FSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEELASDRQQGFEMLFNEVYAQV
                     YDRVLREKQREPEA"
     gene            1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHKUN_002983"
     CDS             1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHKUN_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6661203.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            2427..3757
                     /gene="tssK"
                     /locus_tag="ACHKUN_002984"
                     /pseudo
     CDS             2427..3757
                     /gene="tssK"
                     /locus_tag="ACHKUN_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3762..4523
                     /gene="icmH"
                     /locus_tag="ACHKUN_002985"
     CDS             3762..4523
                     /gene="icmH"
                     /locus_tag="ACHKUN_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO6661204.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4532..7357
                     /gene="tssH"
                     /locus_tag="ACHKUN_002986"
                     /gene_synonym="clpV"
     CDS             4532..7357
                     /gene="tssH"
                     /locus_tag="ACHKUN_002986"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6661205.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESESESEQDNTGAEPADEAGSEQPE
                     ETAETVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEM
                     SVITDLTKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTET
                     VLQLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYS
                     VVLLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHAD
                     DPETMQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFG
                     AEVIIEPEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRL
                     SAVDGAFTADVEDAQNDESVTKDETVL"
     gene            7354..8097
                     /gene="vasI"
                     /locus_tag="ACHKUN_002987"
     CDS             7354..8097
                     /gene="vasI"
                     /locus_tag="ACHKUN_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO6661206.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            8102..9514
                     /gene="tssA"
                     /locus_tag="ACHKUN_002988"
                     /pseudo
     CDS             8102..9514
                     /gene="tssA"
                     /locus_tag="ACHKUN_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            9623..13057
                     /gene="tssM"
                     /locus_tag="ACHKUN_002989"
     CDS             9623..13057
                     /gene="tssM"
                     /locus_tag="ACHKUN_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO6661207.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            13068..14517
                     /locus_tag="ACHKUN_002990"
                     /pseudo
     CDS             13068..14517
                     /locus_tag="ACHKUN_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            14444..14926
                     /locus_tag="ACHKUN_002991"
     CDS             14444..14926
                     /locus_tag="ACHKUN_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6661208.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            14970..>15300
                     /locus_tag="ACHKUN_002992"
     CDS             14970..>15300
                     /locus_tag="ACHKUN_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661209.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKI"
BASE COUNT         3494 a         4299 c         4339 g         3168 t
ORIGIN      
        1 ccggtgcgag ggtgcgcacg ctctccagcc tgcccgtggc actgtagccg tattcccgcg
       61 tctgtcgcgg gccgctgatg cgcaccaggt cgccgttgtc attccacccg tagtcacggt
      121 catacaccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg
      181 tcagttcata tgcggcatta ctgcctgccc tgctgccgaa gctgcgcacc gtctcacggt
      241 gcagcctgtc ccgcgtatac tcgaccagcg gcgtcccgcc cagcttcatg cccgccaggt
      301 aaccgctgcc gtaggtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg
      361 ccagcccctg ttcgttgtag gcgtgtttcg tctcatgctg ccacagcagt tcccccgtct
      421 ccgggttctc caccgtctgc cgttcgcccg tcaggcggcc cttatcgtcg tacccgtagt
      481 ggaccgacac gcggtggcct tcactggtat ggctgagggt ggttaaccac ccgtgctcat
      541 catactgcca ctgctctgcc gggtcgccgt tcaccgtccg gtgcgtgatg cggtccgacg
      601 catcgtagtg ccagagggtg ataagcccct cgtcttcact ctgtgtgagt tttctggtca
      661 ggtcatagtg gtaacgttgc gtccggccgt caaaaccgcg ctgttcagtc agcctgtcaa
      721 ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcacg gtgatgcgcc
      781 cggcagcgtc ataccccatg ctgcgcgtca gaccgccttc cgtcacactc accggacgcc
      841 cgcgtttatc atatgcgatg gtgctgcgtt taccgtccgg tgataccgtt gctgtcaggt
      901 ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccttc tgactgacca
      961 gttgcccacg cgggttataa ctgctgtaag tgctgatgcc ttcctcccgg tgaacggcga
     1021 tttgttgacc gtaccggtcg tactcatacc gcgtcgtgta ccccgagcag tccgtaaagg
     1081 ccagcagttg accgtaacgg ctccatgcca tctgttttgt actgcccgtc gcgtcctgta
     1141 tccctgtcgg cagctcactc gccggccgct ttcaggcatt gcggctgtcc ggccatcggc
     1201 accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg atctgccgcc
     1261 catttatgcc agccccggcg atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg
     1321 ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc tcgcttacca tgagcaattc
     1381 cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg caataaaagg
     1441 tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc gcccgctgga
     1501 agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa tgtttgccga
     1561 aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt
     1621 ccgccaccac gaagaagcta accgggcagc gattgtggag tcgcttcgtg tcctgctgga
     1681 tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca gccatgaact
     1741 gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt acgaagaact
     1801 ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg cccaagtcta
     1861 tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa ggcgtttctg
     1921 gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag
     1981 gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc ggaaataacc
     2041 gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc agccgccgtg
     2101 gatgtgcaac tggtgtacct gactgacgac tcaaaattgc tggcagccga ctatgaccag
     2161 atagccagta cgccgctacc ggatgtgctg ggtaaaaaat atatcgacca ccaggatttc
     2221 aacctgttgc cggacaccat caaaaccctg ccgccggtaa agctggatga gaaaacgcag
     2281 tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa acaaatcgag
     2341 acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca aagcagcatc
     2401 gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg atgtggcagg
     2461 aagggatgct gatgcgtcca caccatttcc agcagcagca gcgttacaac gactacctgg
     2521 ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa ctcactctca
     2581 acaatgaact gctggcacag ggtaagatca tgattgacag cgcgtcaggc acactgcccg
     2641 acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg cacccgcaac
     2701 attttccgga cgagagaagc cgcaatatct acctcgctct gccggtcgcc agtgatgtga
     2761 gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc
     2821 gggatttgca ttcagaagaa ggtgacacgc gaacactgac gctgggacaa ctgacgccgc
     2881 gcatatgagc ggtgcagaag atatgagcgc ctatattacg ctcccgcttt gccgtatcag
     2941 cgatcgccat gccgatggct ccctgacgtt ggatgacgat tttatcccct cctgccagaa
     3001 tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg ccattggcgg
     3061 acgggcaagc gatctggcaa accgtattgg ctctccggcg cagagcggca tcgcggatgt
     3121 ggcggaattt atgatgttgc agttacttaa ccgtaaccag acccggttta cccatcgcgc
     3181 ccgccgatcc cagctccacc cggaagattt ctaccttgat cttgctgggt tgctgggtga
     3241 actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt atgatcatca
     3301 tgatctgacc aaaacattta aaacactgtt accggaagtc aaacgggcgc tgcataccgt
     3361 actgtcgcca agagcggtca atctgccgct gcatctgcgt gacggtatct ggcaggccga
     3421 tatccatgac acagaactgc tgcaatctgc cacctttgtg ctggccgtgg cggcaaacgt
     3481 gccggtcgat cagatccagc gtcagtttat ccagcagtcg aaaatttcct cgccggaaaa
     3541 aatccgcaat atggtcagtg tgcagatccc cggcattcca ttgcgcgcac tgatggtggc
     3601 tccccgccag cttccttacc attccgggtt cagctatttc gaactcgaca agagcggaca
     3661 ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtatccg gcagtttccc
     3721 ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag gggtcactgt
     3781 ctctgccacc atttaccgga tacgacgaaa aaagccagcg cgactatcat ctcgcgcttc
     3841 gtggcaacag cctgaatccg atgatcgacg cagccacgcc gctcctcggt atggtgatgc
     3901 gcctttcaac catgaacagc cagacgatgc cggagcatct ttttgctcag gtggtgacgg
     3961 atgttcaggc cgtggagcaa ctgcttcagg agcagggcta cgaaccgggc gtgattatct
     4021 cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac ggctggtcga
     4081 acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa gcctggggtg
     4141 gagagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt taccaggatc
     4201 tgctggaatt tctctggctc tgtttttctc tgggatttcg cgggcgctac aaagtggcag
     4261 cacaggatca gggggaattt gagcagatct accgccgcct gtaccacgtc ctgcataaac
     4321 tacgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag ggcgggcgtt
     4381 atcagctcat cagtcgcctg accattaaac atattttttg tggtggcgtt gtcgtgctgg
     4441 cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac atcctgcacc
     4501 agctaaacaa attacttcga taaggacgtt tatgatccag attgatcttc ccacgctggt
     4561 aaaacggctt aacctgttct cccgccaggc gctggagatg gccgcctctg aatgtatgag
     4621 ccagcaggca gcggaaatta ccgtcagcca tgtgcttatt cagatgctcg ccatgccacg
     4681 cagtgacctg cgggttatta cccgccaggg cgatattggc atggaagagt tgcgccaggc
     4741 gctgacggtg gagaactaca caactgcccg ttctgcggac agctacccgg cattttcccc
     4801 gatgctggtt gagtggctga aagagggctg gctgctggcg tcggctgaga tgcagcacag
     4861 cgaattgcgt ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc gttatatacc
     4921 gcctgctgcc gcccggctgt tgaccggcat taaccgtgac cgtctgcaac aggactttgt
     4981 gcaatggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta aaggcgcagg
     5041 cacactgacg gacgctgctg acaccctgct tgcccgctac gccaaaaaca tgaccgcaga
     5101 cgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg acctgatgat
     5161 cgacattctc tgccgccgcc gcaagaacaa cccggtggtg gtgggcgaag caggcgtggg
     5221 taaaagcgcc ttaatcgaag ggctggcgct gcgcatcgtg gcaggccatg tgccggacaa
     5281 gctgaaaaac accgatatca tgacccttga cttgggcgca ttgcaggccg gagcgtcggt
     5341 gaagggtgaa ttcgaaaaac gtttcaaagg gctgatggca gaggtcattt cctccccggt
     5401 gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca accagcaggg
     5461 cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc tgaaaaccat
     5521 cgccgccacc acctggagcg agtacaaaaa atacttcgaa aaagatgccg ccctgtcgcg
     5581 ccgcttccag ttggtgaagg tcagcgaacc caacgctgcc gaagccacca ttattctgcg
     5641 cggtctgtcg gcggtctatg aacagtctca cggcgtgctg attgatgatg acgccttgca
     5701 ggccgctgcg acattaagcg agcgttatct ctccgggcgt cagttaccgg acaaagcgat
     5761 tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcgtcgc cgccgaagca
     5821 aatctcggcg ctgaccactc tgagccacca gcaggaggcg gaaattcgcc agcttgagcg
     5881 cgagcttcgc atcggactgc gtaccgacac atcacggatg accgaggtgc tggtgcagta
     5941 tgatgaaacg ctgacggcgc tggatgaact ggaagcggcc tggcaccagc agcagacgct
     6001 ggtccgggag attattgcgc tgcgccagca gttactgggc gtggcagagg acgatgcggc
     6061 gccgttgccg gacgcagata ccgtggagga tacgcagcca gagtcagagt cagagtcaga
     6121 gtcagagtca gagtcagagt cagagtcaga gtcagagtca gagtcagagt cagagcagga
     6181 taataccggt gccgaaccgg ctgatgaagc tggcagcgaa cagccggaag agaccgctga
     6241 aacagtttcc ccggtacagc ggctggccca gctcactgcc gaactggacg ccctgcataa
     6301 cgaccggttg ctggtctccc cgcacgtcga taaaaaacag attgcggcgg tgattgccga
     6361 atggaccggc gtgccgctca accgcctatc gcagaatgaa atgtcggtca tcaccgacct
     6421 gacgaaatgg ctgggtgaca ccatcaaagg ccaggacctg gcgattgcca gcctgcataa
     6481 gcatctactg accgcacgcg ccgacctgcg tcgtccggga cgcccgctcg gtgcgttcct
     6541 gctggctggc cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg cagagctgct
     6601 ctacggcggt cgccagtacc tgaccaccat caatatgtcc gaattccagg agaaacacac
     6661 cgtctcgcgg ctgattggtt cgcctccggg ctacgttggc tacggtgaag gcggcgtact
     6721 gaccgaagcg attcgccaga aaccctactc ggtagtactg ctcgatgaag tggaaaaagc
     6781 gcacccggat gtgctcaacc tgttctacca ggcgttcgat aagggcgaaa tggcagacgg
     6841 tgaaggccgc ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca acctcggcta
     6901 ccaggtaata gtcgagcatg ccgatgaccc ggaaaccatg caggaagtac tgtatccggt
     6961 gctggccgac ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc cgtacctgcc
     7021 gctgtcgaaa gagacgctcg ccaccatcat tgccgggaaa ctggcccgcc tggataacgt
     7081 gctgcgcagc cgctttggcg cggaggtgat tatagaaccg gaagtgacgg acgaaatcat
     7141 gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca tcgacggcaa
     7201 tatgctgccg ccgctctccc tgctgctgtt gcagaaaatg gcggcgaata cggcggttgc
     7261 ccggattcgg ttgtcggcag tggacggcgc atttacggca gacgtggaag atgctcagaa
     7321 cgacgagtcc gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt tttccggcag
     7381 acaggatggt ttatcgcggg cctgatgacg ggcttacccg ccactgccgc gcccgcagaa
     7441 gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc ggatgcgaca
     7501 gccacgttac aggccatgca gtcctgtcgg cgggagtccg cggcgctgga gcggcttgac
     7561 tgctatgacc gcctgctggc accgctgtcc ccgtcagggt ttgacggggc gctggtcaaa
     7621 gccggttttg tgggcgaggc ctggacccgc gccacagagc aggaaaaacg ccgcgagggc
     7681 aacaccacag aactgctggt gacgcaggtg ccgggggagc gcccgacggt ggtgataacc
     7741 accccggcta tcggtcatgt tccgccacgc ccggtgctga tgttcagttg cgtggacaat
     7801 atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat cgccgtcact
     7861 ctgaatgccg acaaccgggc attgcgcagc cactggtttg tgcgcgagaa cggcaccctg
     7921 ctggagtcca gccgggggtt atccgggatt gatgaaatta agcaactgtt cggcgcaaaa
     7981 acgctgacgg tggataccgg cgcagacaat gccgccggaa agctgacttt taacattgac
     8041 gggctggcaa gggcgattgc tcccctgcgc gatgcctgcc actgggcggg agaataaaaa
     8101 tatggcaatg gacttacgcg atccgaatgt ctggatatcg cacctgctgg aaaacctgcc
     8161 ggaagagaaa ctggcatcgg cgctgaaaga tgacaacccg aactgggagt atatcgacgg
     8221 cgaaatcgtc aagctgggat cgctggccca cagtcaactg gatatcccgg aactccagcg
     8281 ccgtgggctg gtcattctgg cttcggagag taaggatttt cgtctgctgg cgcatctgct
     8341 gagaaccctg caacacgccg gtgatcctca tctcgcattg cgcctgctgg cgctgtatgt
     8401 ggagcattac tgggcggtgg ctgcgccgca gaatatggcg cataaaaaac gctttgccag
     8461 ccaggtcatt aaacgctttg aaacaggtat tgaagttttt tcacagaacg ccgccacagc
     8521 gcagcgcgat gcgctgtcag gggaactggc aaaactggcg caatgctggc agtcacataa
     8581 cgccccggaa ctggcacagg ctactgatga tctttttgcc ctgtaccagc gtgcgtttaa
     8641 tcgtgcggca ccggctcctg tccccactcc ggcggcctcc ggtagttcac cacagaccac
     8701 cgccacgtct gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa tagtcatcga
     8761 cagtcacgac gacaaagcct agcgcgacac gctgttaaaa gtggcggcca ttttatgtga
     8821 acgccagccg gattcaccgc agggctaccg tctgcgccgc catgcgcttt ggcagaacat
     8881 caccagtacg ccccaggcgg aaagcgatgg ccgtactcca ctggctgcgg tctccgccga
     8941 tatggtggca gattaccatg ctcagctcgg tagtgccgat atggcgctgt ggcagcaggt
     9001 ggagaaaagc gtactgctgg ctccttactg gctggatggt cactgccttt ctgcacagac
     9061 ggcgctgcgt ctgggttaca aacaggtggc agacgccatc cgcgatgagg tcatccgctt
     9121 ccttgagcgt ctgccccagc ttaccgggct gctgtttaat gaccacacac cgtttatcag
     9181 tgagcagacg aaacaatggc tggcagcttc gcccgacgcc aaagttgcac cggttgcgca
     9241 aatcggtgag gaatcgaagg cagccagagc ctgttttgct gagcagggtc tggaggcggc
     9301 gctgcgatat ctggacatgc tacccgaagg cgatccccgc gatcagtttc accgccagta
     9361 ccttgccgca cagttgacgg aagaggcggg gctggtacag cttgcgcagc agcagtaccg
     9421 gatgttgttc aggatgggat tgcagatgat ggtggcagac tgggagccgt ccctgcttga
     9481 gcagctagaa caaaaattca cggcagaaca ataaaagaga acaggagttt ttgtgttcag
     9541 attaccaaca ccccgactac tcagcggact caaatcagcc ctgcgaccgg cgatgccccg
     9601 gtttaaagtc tctgctttct ggctgctgat actggcgtgg atttttctgc tggtgtggat
     9661 ctggtggaaa ggcccgatgt ggacgctgta tgaagaacag tggctcaaac cactggcgaa
     9721 ccgctggctg gcaacggcgg cgtgggggat tattgccctg gtgtggctta ccgtccgggt
     9781 gatgaagcgc ctgcaacagc ttgaaaaaat gcaaaagcaa cagcgcgagg aagccgttga
     9841 tccgctcagc gtggaactga acgcccagca gcgttatctt gaccgctggc tgctgcgcct
     9901 gcaacgccat ctcgacaacc gccgtttcct gtggcagttg ccgtggtata tggtcatcgg
     9961 cccggcgggc agtggcaaaa cgacactgct gcgcgagggg tttccgtccg acattattta
    10021 tgccccggag ggcgcacgcg gcgcagaaca acgcctgtac ctcacgcccc atgtcggtaa
    10081 acaggcggtg atctttgata tcgacggcac actctgcgct cccgctgatg cggatatcct
    10141 gcatcgccgt ctgtgggaac atgccctcgg ctggctgaag gaaaagcgcg cacgccagcc
    10201 gctgaacggg attattctga cactcgattt acccgatctg ctgaccgcag acaaacgccg
    10261 ccgcgagcat ctgttacaga cgctgcgcag ccgcttacag gatatacgcc agcatcttca
    10321 ctgccagtta ccggtttacg tggtactgac ccggcttgat ttattacagg gttttgccgc
    10381 cctgttccag tcactcaaca gacaggatcg cgatgcgatt ctgggcgtca cgttcacccg
    10441 ccgcgcccat gaaaatgatg actggcgaac agagttgaat gctttctggc agacatgggt
    10501 ggatcgaatg aatctggcgt tgccggatct gatggtcgct cagactcaca cccgcgcgtc
    10561 tttattcagt ttttcccgcc agatgcaggg aagccgtgaa ccgctggtgt cactgcttga
    10621 gggtctgctt gatggcgaaa atatgaacgt gatgctgcgt ggtgtctatc tcacctcttc
    10681 gcttcagcgt gggcagatgg atgatatatt cacccagtct gccgcccgcc agtaccggct
    10741 gggcaataac ccactggcgt cctggcccct ggtggacacc gcgccttatt tcacccgcag
    10801 cctgttcccg caggcattac tcgcagagcc taatctggca acagagagcc gcgcctggct
    10861 gatacgttcc cgtcgccgcc tgacggtttt ctctgccaca ggcggcgtgg cagcactgct
    10921 gctcatcacc ggctggcatc actattacaa cggtaactat cagtccggca tcaccgtgct
    10981 taagcaggcc aaagccttta tggacgtgcc gcctccgcag ggggaagatg actttggcaa
    11041 cctgcaactg ccgctcctga acccggtacg cgatgccaca ctggcctatg gcgactgggg
    11101 cgaccgcagc cgtctggccg atatgggact gtaccaggga cgacgtatcg ggccttatgt
    11161 ggaacagacc tatctgcaac tgctggagca acgttacctg ccctcgctgt ttaacgggct
    11221 ggtcaaagcg atgaacgccg cgccgccgga gagtgaagaa aaactcgcgg tgctgcgcgt
    11281 aatgcgaatg ctggaggaca aaagcggacg taacaatgag gtggtgaagc agtatatggc
    11341 aaaacgctgg agcgaaaaat tccacggcca gcgcgatatc caggcacaac tgatgtccca
    11401 tcttgactac gcgctggctc atactgactg gcatgcagag cgtcaggcgg gcgacggtga
    11461 cgccatcagc cgctggacgc catatgacaa gcccgtggta tcagcacaga aagaactgag
    11521 caaactgcct gtctaccagc gggtttacca gagcctgaaa acgcgggcgc tgggcgttct
    11581 tcctgccgac ctcaatctgc gtgaccaggt agggccaacc tttgaccagg tgtttacatc
    11641 tgccgatgac aacaaactgg ttgttccaca gtttatcacc cgctacggcc tgcaaagcta
    11701 ttttgtaaaa cagcgcgatg aactggttga actgacagcg atggattcct gggtacttaa
    11761 cctcacccgc aatgtgaaat acagtgacgc cgaccgtgcg gaaatccagc accagttgac
    11821 cgagcagtat atcagcgact acaccgccac ctggcgggct gggatggaca atctgaatat
    11881 ccgcaatttt gagtccatcg gacaactgac cggggcgctg gagcaggtta tcagcggcga
    11941 ccagcctttg cagcgggcgc tgaccgtgct gcgtgacaac acacagccag gcgtcttttc
    12001 tgaaaaactc tctgccaaag aacgggagga agccctggca gagccggatt accagttact
    12061 cacccgcctc gggcatgaat tcgccccgga aaacagtacc ctggcagtac agaaagacaa
    12121 agaaagcacg atgcaggccg tgtatcagca actcaccgag ttgcaccgct acctgctggc
    12181 aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt tacggcttga
    12241 tcagaacagc agcgatccga tattcgccac ccgccagatg gcaaaaacgc tgcctgctcc
    12301 gctcaaccgc tgggttggca ggctggctga ccaggcctgg catgtggtga tggtggaggc
    12361 tgttcattat atggaagtgg actggcgcga cagcgtggtg aaaccgttta acgagcaact
    12421 ggcaaataac tatccgttta atccgcgttc tgcacaggat gcctcactgg atgccttcga
    12481 acgcttcttt aaaccggatg gcatactgga taccttctac cagcagaacc tgaagctgtt
    12541 tatcgataat gacctgagtc tggaggatgg cgataacaac gtcattattc gcgaagatat
    12601 tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca aacagaacgg
    12661 tctgggaaca tcctttgccg tggaaacggt atcgctttca ggcaataaac gccgcagtgt
    12721 actgaacctt gacggtcagt tagtcgatta cagccagggc cgtaactata ccgcccatct
    12781 ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca tcggcaccag
    12841 cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc gcctgttcgg
    12901 ggccggacaa ctgaccggag tacaggatgg caactttacc gtgcgcttta gcgtggacgg
    12961 tggcgcgatg acctaccgtg tgcataccga cacggaagat aacccgttca gcggtgggtt
    13021 gttcagccag tttggtctgt cagacacact gtactgagga ggcagggatg aatagtaacg
    13081 tactgacaca aactatcgtt accggcagtg acccgcgcgg gctgccggaa ttcagcgcca
    13141 tccgcgagga aataaacaaa gccagccacc cgtcacagcc tgagctgaac tggaaactgg
    13201 tggagtcgct ggcgctggcg atttttaaag ccaacggtgt ggatttacac accgccacct
    13261 actatacgct tgcccggaca cggacacagg gactggcggg attctgcgaa ggtgcggaac
    13321 tgctggcggc aatggtaagc cacgactggg ataagttctg gccgcagggc ggcccggcgc
    13381 gtactgaaat gctggactgg tttaactccc gaaccggcaa tattctgcgt cagcaaatct
    13441 cctttgcgga atccgaccta ccactgatct accgcacaga gcgggcattg cagcttatct
    13501 gcgataagct ccagcaggtg gaactgaagc gcgttccgcg cgtggagaat ctgctctatt
    13561 ttatgcagaa cacgcgtaaa cggcttggac cccagctgaa gagtaacact gagaacgccg
    13621 cacagaccac ggtcagaacg ctgatttatg ccccggaaac acaggcatct tccacaccag
    13681 aagcggtagt gcctcccctg cccggcctgc ctgagatgaa agtggaagtt cgcagtctga
    13741 cagagaatcc cccacaggcc agtgtgataa atcaaggcag tacggtaaga gggtttatcg
    13801 cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag gtctatccgg
    13861 tgcagcagca actgttacag gttaacgaca ccgctcaggg cgcagcaacg gtgtggatgg
    13921 cctcacctga actcgaaaac tatgagcgca ggctgcaaca acttcttgat acctccccgg
    13981 tacagccgct ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc tggccggaaa
    14041 gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc cgcgcacaga
    14101 gtagcccgca gttgcgtggc tggttgcaga cccgccagga cttacatgct tttgcagatc
    14161 tagtgatgca gcgcgagaaa gagggactaa ccctttccta tatcaaaaat gtcatctggc
    14221 aggcggagcg gggactgggg caggaaacac ccgttgagtc tctgttgacg cagtaccatg
    14281 atgcccgtgc gcagaagcag aatacagatg cgctggaaaa acaaattaat gagcgactcg
    14341 aaggcgtgtt aagccgctgg ctgctgctga agaataacgt catgccagag gcggcaaccg
    14401 gtaccacggc cgaaaaataa ttatttaatt aaaggagatt atcatggcaa atataagtta
    14461 tttatctttg agtggagaga cacaagggct aatttcagca ggttgctcaa cgcttgattc
    14521 tgtcggaaac aaagcgcagc cagaacataa agatcagatc atggtttatg cgttaatgca
    14581 ttccatctca agaagtcaaa atgtcaatca tcatgaatta attatcacca aaccggttga
    14641 taaatcctca ccattgctgg caaaagcact tagtgacaat gaaaaaatgg caatatgtga
    14701 atttatactc taccgaacca gtaaagcggg tatttaccag ccttattata aaataaatct
    14761 gtcaaaagcc cgaatatcaa gtattgattt tgtcacaccg catgctgtcc tggaaaaaga
    14821 actggaacca caggagagaa ttgcctttat ttatgaagat atttcctggg agcatacgtt
    14881 agccggtacg aatgcaatga gtaaatggca ggatcgtgta cagtaactta ctgtaataac
    14941 ataataaatt actattgcgg agagataaaa tggctggttt aaggaataac aacaacacac
    15001 agaatgcaca gtgggctgat tatgtgggtg acattcttcg gggggctcag ccgataaacc
    15061 agctagtacc tcagcaccct tatcttaacg acgtcccact cattgatgag ttaaggcatc
    15121 agaacactca tcatgttatt ctcttgacac ttgatgttgc aaaaaaaata ctcagcccaa
    15181 taacatcatt tgattacatt cattttatca ctacacatcc ttcaggtata aaagatacac
    15241 tcgcctggtt agttaacgcg ggtaaattaa tgactgagtt tgatgacaat gggaaaatta
//
LOCUS       DAZFRJ010000107        15230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000107 DAZFRJ010000000
VERSION     DAZFRJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.108"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(63..464)
                     /gene="crl"
                     /locus_tag="ACHKUN_002993"
     CDS             complement(63..464)
                     /gene="crl"
                     /locus_tag="ACHKUN_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6661210.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(522..1766)
                     /gene="frsA"
                     /locus_tag="ACHKUN_002994"
     CDS             complement(522..1766)
                     /gene="frsA"
                     /locus_tag="ACHKUN_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6661211.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(1858..2316)
                     /gene="gpt"
                     /locus_tag="ACHKUN_002995"
     CDS             complement(1858..2316)
                     /gene="gpt"
                     /locus_tag="ACHKUN_002995"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6661212.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2577..4034
                     /gene="pepD"
                     /locus_tag="ACHKUN_002996"
     CDS             2577..4034
                     /gene="pepD"
                     /locus_tag="ACHKUN_002996"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6661213.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4091..4705)
                     /gene="prfH"
                     /locus_tag="ACHKUN_002997"
     CDS             complement(4091..4705)
                     /gene="prfH"
                     /locus_tag="ACHKUN_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6661214.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(4702..5841)
                     /locus_tag="ACHKUN_002998"
     CDS             complement(4702..5841)
                     /locus_tag="ACHKUN_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6661215.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(6087..6539)
                     /locus_tag="ACHKUN_002999"
     CDS             complement(6087..6539)
                     /locus_tag="ACHKUN_002999"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6661216.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(6536..7591)
                     /gene="dinB"
                     /locus_tag="ACHKUN_003000"
     CDS             complement(6536..7591)
                     /gene="dinB"
                     /locus_tag="ACHKUN_003000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6661217.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7662..8585)
                     /gene="lafU"
                     /locus_tag="ACHKUN_003001"
     CDS             complement(7662..8585)
                     /gene="lafU"
                     /locus_tag="ACHKUN_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6661218.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(8588..9451)
                     /gene="motA"
                     /locus_tag="ACHKUN_003002"
     CDS             complement(8588..9451)
                     /gene="motA"
                     /locus_tag="ACHKUN_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6661219.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(9464..10180)
                     /locus_tag="ACHKUN_003003"
     CDS             complement(9464..10180)
                     /locus_tag="ACHKUN_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO6661220.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(10200..10667)
                     /locus_tag="ACHKUN_003004"
     CDS             complement(10200..10667)
                     /locus_tag="ACHKUN_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO6661221.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(10675..11736)
                     /locus_tag="ACHKUN_003005"
     CDS             complement(10675..11736)
                     /locus_tag="ACHKUN_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6661222.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(11733..12044)
                     /locus_tag="ACHKUN_003006"
     CDS             complement(11733..12044)
                     /locus_tag="ACHKUN_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO6661223.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(12049..12441)
                     /gene="fliS"
                     /locus_tag="ACHKUN_003007"
     CDS             complement(12049..12441)
                     /gene="fliS"
                     /locus_tag="ACHKUN_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6661224.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(12464..13780)
                     /gene="fliD"
                     /locus_tag="ACHKUN_003008"
     CDS             complement(12464..13780)
                     /gene="fliD"
                     /locus_tag="ACHKUN_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6661225.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(13987..14901)
                     /gene="lafA"
                     /locus_tag="ACHKUN_003009"
     CDS             complement(13987..14901)
                     /gene="lafA"
                     /locus_tag="ACHKUN_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO6661226.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
BASE COUNT         3488 a         4125 c         3791 g         3826 t
ORIGIN      
        1 gttggcatga cctatcagtt ttaatgatta ttgccggatg tgatgcatcc ggcacatttc
       61 actcacgccg ttaacttcac cggctcgtca cgaaaatcat ccgccggttc cagcttcaga
      121 ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact cacgcagggt gtgctccagt
      181 cgttcaacca cttcagtgtc ttttaccgga acactcttcc agtcgcctgc tttatcgaac
      241 agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc catccaccag
      301 ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc cagacaatcg
      361 aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag tgcggtaaat
      421 tttttgatca atctgctctt cgggtgtcca ctcggtaacg tcattgctat ctcctgttgt
      481 gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa gcgtttttcg
      541 atccagccgg tgatttcctg aagacctttg tcaaaattcc gatacaccgg gttaaatggg
      601 atctctaata atttaccgtc agcagatgat gaggtgatta agcgtgagtc ctcttccggg
      661 ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg acgccccagc
      721 aatccttgca cttttaatga atagcgattc agctccacgc gtaacgcgtc atcggaagca
      781 tcatgcatcc ccaaacgact cgccagaacg tcaagataca tttccggcac ctgttgctgg
      841 cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac cgctttcaga
      901 cgcggcgatt caagatatgc cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag
      961 gccgcgacgc gagtgtgatc cacccacggt acgttaggca gcgcctttaa gacgtgctga
     1021 tgcaacaggc tggagtcctg ggtgagcttc cattttgaag aaaagcccac cgatggcata
     1081 tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac gttcatacag gctgtaatag
     1141 tccgtctgca tcgcatccag accaccacac attaatactg tcgggaacgg gccatcgcct
     1201 ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgc
     1261 cgcatcgtgc ccggcagacg ctgggcggcc tcttcatagg cgcggtttga taaagcctgc
     1321 gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt gtacaacgta
     1381 gccgcatgca gccagtgtcg accactgagt tgcggatctt cttccgcaca ggctttttgt
     1441 tgccacacca tcgcctgggt ggcccactca taaatccagt tgccaccacg gtagccaatc
     1501 acagtatcgt ataaatcatc gtcggtacgt tcagcatcgc tcatcacaat acgtgcctgg
     1561 acgtcgagga tttcacgtgg gtcaataccg cgccagatcc acatcagacg gttaatcatg
     1621 cgataccagt gagggatggt tttaccatca agagccgact gcacaggcgg ttgtgcgccg
     1681 tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg
     1741 gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac tggaaactat
     1801 tgtaacctgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt gaaaagatta
     1861 gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt cgatccaggt
     1921 atcttgcggg atatcaacaa catagtcatc aaccagcgga cgaccagccg gttttgcgaa
     1981 gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaacagcag taccaccggt
     2041 atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac
     2101 tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat cgacatgacg
     2161 aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc ttacggcaat
     2221 aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg catggatctg
     2281 caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag cctgtttatc
     2341 tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga gatctggcgc
     2401 actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc tccctgacgc
     2461 gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga caacattttc
     2521 tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga cttaacgtgt
     2581 ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa atctgttcta
     2641 ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt tgggcaaaag
     2701 agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt aaacctgcta
     2761 ccgcaggtat ggaaaatcgt aaaccggtcg tcttacaggc ccacctcgat atggtgccgc
     2821 agaaaaataa cgacaccgtg catgacttca cgaaagatcc tatccagcct tatattgatg
     2881 gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcaga taacggcatt ggtatggcgt
     2941 ctgcgctggc ggttctggct gacgaaaacg tggttcacgg cccgctggaa gtgctgctga
     3001 ccatgaccga agaagccggt atggacggtg cgttcggctt acagagcaac tggttgcagg
     3061 ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg ggttgtgcgg
     3121 ggggtatcga cttcacctcc aacctgcatt tagatcgtga agcggttcca gctggttttg
     3181 aaaccttcaa gttaacctta aaaggtctga aaggcggtca ctccggcggt gaaatccacg
     3241 ttggcctggg taatgccaac aaactactgg tgcgcttcct ggcgggtcat gcggaagaac
     3301 tggatctgcg ccttatcgat ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag
     3361 cctttgcgac cattgctgtc gcagctgata aagtcgacgt cctgaaatct ctggtgaata
     3421 cctatcagga gatcctgaaa aacgagctgg cagagaaaga gaaaaatctg gccttgttgc
     3481 tggactctgt agcgaacgat aaagctgccc tgattgcgaa atctcgcgat acctttattc
     3541 gtctgctgaa cgccaccccg aacggtgtga tccgcaattc cgacgtggca aaaggtgtgg
     3601 tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga caataacgta gaaattcact
     3661 gcctgatccg ttcactgatc gacagcggta aagactacgt ggtgagcatg ctggattcgc
     3721 tgggtaaact agctggcgcg aaaaccgaag cgaaaggcgc atatcctggc tggcagccgg
     3781 acgctaattc tccggtgatg catctggtac gtgaaaccta tcagcgtctg ttcaacaaaa
     3841 caccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc
     3901 cggaaatgga catggtttct atcgggccaa ctatcaccgg tccacactct ccggatgagc
     3961 aagttcacat cgaaagcgta ggtcattact ggacgctgct gactgaactg ctgaaagaaa
     4021 ttccggcgaa gtaattattt gatttgctgc cggatggcgt ttaatcgcct tccggcagtt
     4081 tcatccttca ttatccttcg ataaaagcca tccctgtaaa tgtccgtctc gggttgccac
     4141 gttcaatctg gtgatggaac attcgccgca gcgatttcag caccgcgcta ttttcctgtt
     4201 gctgctgctc cagcttccag gcaatcagca atcgtgccag ccgcttgtta gcatgctgac
     4261 tacgctctga ctgaaccttc acgctaatac cggatgccaa atgcgtggcg cgtaccgccg
     4321 agtcggtttt attgacatgt tgaccgcccg gccccgacga acgcaacgtc tcataacgga
     4381 ttgcatccga ttgttcctgc tcatcagcgg taaaacgccc aatgcccaga aaccagtttt
     4441 tgcgcccatg atgaggccga tacggactcg gacaaatcca ctgaatagtg ccacaccagc
     4501 tttcgcttaa tgcccatgcg ttatcgccat ccagagaaat cagcgccgaa cgcagcgtgt
     4561 cagagtagtg gcccgtttct gtttccagca ccgttaccgc gacgtcttgt cgggcagctt
     4621 ctttaattag cctgtccagc gcctttttca ctgcgagaca acattcttcc ggcccctgcg
     4681 ctgaggagag ttgtagcaag atcatccttt tttccctcca ctgtttttga gcgttagcac
     4741 cggacgcagc cgcgcgacag gaataattaa accggcctgc accagacatt gcaccacgct
     4801 ttcagccgat ttataggctt gtggcgcttc ttcaaagatg agttgtttat cgcgacaaat
     4861 tacccgactg ccaagttcag tacgcgagag ttgcgtcgcc gtgtatttcg ctgccagacg
     4921 ccctttacac tcggtgcgcc cccatttacg ccctgcccca tgcgccagcg agtggagcgt
     4981 tttttcgttc gcaacaggct gtacaatcca ggagtaatca ccacgtgaac cgggaataat
     5041 caccagaccg ttgtcatccg gcgtggcacc tttacgatgc aaccagccct gttgatcgcc
     5101 gatttgacac gcgctaacaa agttatgcgc cacatccaga accggacttc ccgtggcctt
     5161 aacttgttgc attatgcgca aagcgatcat ctggcgatta atacacgcaa acgccagcgc
     5221 atcgtcatgt tccgcaatat accgtagcgc gtcgtcactg ccttgaggca aaccatgatg
     5281 cgagaacgag gcaatatgcc gctgtaaaat agactgcccc accccccgcg agccgctatg
     5341 aaccaacagt tgcagatgtt gtgcatccag acctgccagt gcaaacagtt cagcgttgat
     5401 gatttgatca acttgttgca gttcagcgaa atgattgccg ccaccgatag agccaagcga
     5461 gctgcgccac ggatgctgtg caaatgctga cggcaggttt tcctccagcc agctttcttc
     5521 agcaacgtca tccagatctg ataatcgctt ttcaaactta tcggcgttgt atttgcgagc
     5581 gagaatatct gtttgccata gcgccatacc gcagccgata tcattgccga ccagtgccgg
     5641 ataaaaacga ccaacagaga agaacgctgc gccaatcgga tagccgcgtc cgggatgtaa
     5701 atctggcatc cccactacgc gctgcatgtt gggtaaattt gccgtggtgt gtaattgttg
     5761 gatcgctaaa ctctcgatcc acaggtcatc agatgcgatg gtaaataccg catcggataa
     5821 gggacgaata taattgccca tataccattc cgtaaataga ctcgtcatac ttcaagttgc
     5881 atgtgttgcg acttgaatta ttgagagtaa caattaaaaa atcaggaaat ggcagacgaa
     5941 tccagggcaa gaaaaaaaga attaatcctg gaaaggtctg caaagagtca ttgataaagg
     6001 atttgtcatg atttacctcc ttgcagtttg tggaataaga aaacgcgcac atactaggct
     6061 tgcgttattt tcattgcaag ctggatttaa tgttgctgtt tataacgcat ataaaaatta
     6121 ataaaccact ctccccgaca ttcaacgcgt tgctgcttaa ctgtctgaaa accataacgt
     6181 tcaaaaaagg gttttgcggt tatgcttgcg tccaccgtaa gttcggattc agacttaatc
     6241 aaaggtttta acaaagcgct ggcaacccca cggcgggtgt attcagggtc aacaaataac
     6301 atatcgatat agtgttcaac gcaggtaata aagccgacca gttttgcatt aatgactgca
     6361 acccgcactt gtgatttcgc gagtttctcc ttccagcgag attcgtcaat ctgcgcccag
     6421 gcggcaattt gttgtggtga ataatgctga ctggcagtca tcgtaaccgc tctaatgaaa
     6481 atagcgcata gttgctgaaa atcgccaggc tgatagtctc ttatttgtat gttattcata
     6541 atcccagcac cagttgtctt tccatttgcg ggtcaagcaa cgtcacatgc agccccacca
     6601 gtcgcacacc gcgcccgccg cggcgttcat cccaggtttt acgcgcggtg gcgattaagt
     6661 cagctttatt cagccgcggc cagacgtgct cctgagtggt ttgctgaaaa tcatcaaact
     6721 ttaatttcac tccctgacgg gcgatcagta aatcaggttt tacctttgcc agacggcgtt
     6781 caagttccgg atacagccgc tcgataatcg cttcacattc agaccagtga tgaatatctt
     6841 ccgccatcgt gcgttccacg ccgacggatt ttcgtaaccg ttcgctgtta acgtcgcgct
     6901 cgtcaatccc ctgactacgc tcccacaaaa tgcggccaaa tttgccaaag cgtttaagca
     6961 gcatcaccag atcacacttt tgtacatcac cgcaggtccg cagccccatc gcttccagtt
     7021 ttgccgccga gactttgccg acgccgggga tttttgccag cggtaaggtt tgtaaaaatg
     7081 ccggaacttc tgccggcgta atcacaaact gaccgttggg tttattcatg tcggaggcga
     7141 ttttggcgag aaactttact ggtgccacgc ccgcagacgc tgtcagttgc agctcgctga
     7201 aaatcgtctg gcggatttcc tgggcgatga gggtcgcaga accgtggcaa tggacgctat
     7261 cggtgacgtc gagataagcc tcatccagtg acaacggttc aatacgcgag gtgtagcgcg
     7321 agaagatttc acggatatga tttgaggctt ctttgtaggc gtcaaagcgc cccggaagca
     7381 aggtgagatg cgggcataat ttgagcgcca tccctgtcgg catagcgcta cgtacgccaa
     7441 atttacgcgc gggataattg gcggtgctga tcaccccccg acgttcgcgg ctgccgccaa
     7501 tagcaatagg gatatcgcgc agggcgggat tgtcgcgcat ctccaccgct gcgaaaaagc
     7561 agtccatatc cacatgaatg attttacgca ttactcacct ctcaacactg gtaaagtata
     7621 cagtgatttc agggtttgag aaatgcgtaa agattcagca tttaacgcgt tcgctgcgaa
     7681 agcacctgct gcttatccag cttttgcacc agcggctgca ccactttatc cccatgctga
     7741 ccaaagtatt gatacagcgt atcggacgca cttttggtca gcaccataat ctcaatgcgc
     7801 cggttgcccg cgctttgcgg atttttggaa tccagcagca tctggtccgc cattgcgctt
     7861 acctgcatca ctttatcttc cggcattccg gcctcttcca gcacccgacg agccgaaagc
     7921 gcgcggtcac ccgaaaggtt ccagttgttg tagatattgt ttttgtaagc catcgcatcg
     7981 gtatgcccgg taataataat tttgttatcg agcgagtcga aaactggcgc gagctccacc
     8041 agcagcgttt taaagaacgg cataatctgc gcgctgccgc gttcgaacat attgcgattc
     8101 tggtcgtctt taatcagtac gcgtaatccc tggggaacaa tctccatctc cagattcgct
     8161 tccatatgcg catcacgggc aatggtgttg atactggttg ccagctcgcc aagctcggtg
     8221 gcggatttct tcttcagcag cgccgttttc tcattcacat cacgcgcttt tttctccgtc
     8281 tcttccggca ccgccacggt ggcaggtttt ggcggatgag acgggctgat tttatttaac
     8341 ggcgacagcc cgccaccgtt aaaaatcgac tgcccgtgca gcgcggcgat aatgctttca
     8401 cgctcggatt tactgacgct gttgacaatc cacagcgtca taaacagcgc catcatcgcc
     8461 agggtaaagt cggcgaacgc caccttccac gccccgccat gatgcccggc atgattcttt
     8521 ttgatctgcc gccggacgat ggtggttttt gccccacgct cgcggcggtt gcccgtcttt
     8581 ctcatgctta ttcttgctcc agcatcgcat tcacccacgc gtcgaggttg gcgaaggtcg
     8641 gtttggacgc cagatgcagc agcttacgcc cggcatccac cgccagcagc gtcggtttgc
     8701 cgcctgcctg cgccaccagc acagtgcgca cgcattccag caaagaatgt tctgcccgcg
     8761 cctgttgttc catggcgttg gcgagcgggt ccatcaggca gtagcagata aagacgccta
     8821 aaaacgtccc caccagcgct gccgccactt tcaggccgat cagggcaata gagccgtcaa
     8881 tcgactgcat ggtgatgatg atccccagca ctgctgcaca aataccaaat cccggcatcg
     8941 cttcggcggt acgttgcagc gagcgcgacg gcgtgagcag cgactcttcc gcagtgtcca
     9001 gttcctgatc gagaatgcct tccagttcat gggcgtcgat tttacccatc gccatcaggc
     9061 gaaagttatc ggcaataaag gtcaccagcc gcttctgggt taacaccagc gggtatttct
     9121 ggaaaatggt gctctcttcc ggttgctcaa tatgttgatc cagcatgcgc agaccaccgt
     9181 tttgcaccat ttcgagcaac tcatacaggc acattaatag ctgacgctga aactccggcc
     9241 cgagctgttt tttactgatc acacctttaa tttgatgggc aatctctttc agcacatgtt
     9301 tcgggttacc aataatcatc gccccgaaac cggcacccaa aataatgatc atctccgccg
     9361 gttgccagat agccactaat tgcccgcccg cttcaaaata accgccaaat acgcagccga
     9421 gcaccaccag taaacctaat attttttgca tgatttcttc actttaattc ggatagacaa
     9481 aatcacgaat tttctgcgcg atctgtttat tcagttgaca aattcgggcc tcggttagcc
     9541 ctaataccag cgcaatctct ttgagattca tctcatgctg gtaatacatg gagaggatca
     9601 gttgctcttt ttcgcttaat tgtgaaagcg cctgttgcag catttgctga gtgacaaact
     9661 gatcttccag ctcccggcca acaagcggaa tgcctggccc ttcgccgttc agcagttcgt
     9721 ccaggctggc gagcgtttcc gccccttcca gttgctgata ttcgagataa tcttcatgtg
     9781 agatcccttc cacggccagt tctgaccact ccggttcgtg ccccagtttt ttgcgcgttt
     9841 cgcgaatcag atctttcatc tgatgatatt tctgtcgcag ctggcgcggt cgccagtcga
     9901 gagcgcgcag ctcatccagg atcgcgccgc gaatacggtg cacggcatag cccgcaaaac
     9961 cttcgtccgg cagcccgtag cggcggatgg cattaagcag ccccattaac gccgtttgtt
    10021 ccatatcctg acgatcaata atgcaattac actgcggggc aagttgccgc accactttac
    10081 gcaccaacgg taaataggcc tgcaagtagt ggctctcctg ctgcggggtt aataccggcg
    10141 cggcagcaaa ttcttctgac tcgaatacat cctgtagcat gtttatatcc gcctggtgat
    10201 tactggaaga ccactttttt caccagcaaa tccttatacg gtgcgctcat tttgcgacgc
    10261 tgaagatctt tctttaacgc gtcagaaatg agtttgcgga tggcagatat tttcatgccg
    10321 cgcaaatctt caaatttcat ttccgtcagg cattcaaccg caatactttg atacagcggc
    10381 tcctgatgtt ttattttttc cgcttctttg tcactggcga ccaccatcac taattccgcc
    10441 gacaaataat ggtccgcgcc gttgttgtca tgcagcgtga caatggtttc cggcaacgtc
    10501 acaaacacgc ttttgctttc atccagggtt tccaccgttt ccgtctgcgc ggcggcagtc
    10561 ggttttcctt ttccggcata atgatgccag acaccccatc cagctccggc ccccaccacc
    10621 aacgccagaa cggcgctgac cacgcccgcc accacgattt tcttcatatt gcctttacac
    10681 tttgatcaaa acggattcgt tctgttgaaa ttgaggcgca tcatcaagct gtaaggcggc
    10741 actcacctct tcctgctgat ggcccgactg ttgttgttgc tgcgactgcc cttcggagga
    10801 aacctgtacg ttcacctcca taaaattttg cgccgtcaga tgctggcgga gatcgtcgct
    10861 aaactgctgt aacgcgcggc aaacttcact ttggttcgcg ccgatgtgca ccatcagttt
    10921 gccgttgtcg agttgtacgg cgatctcgag cttgccaagc gacggtggat cgagacggat
    10981 ggtggagatc tgctgttgtt tgttgagctg aaagtggatc tgatctttca aaagcgtcgt
    11041 cagtttttcg cccagttcgt ccatcgaaag cgtggctggt ttggcgacaa tctcctgatg
    11101 atcgctgagc agctccggcg tcttaacctg caacaccgca cgcgcaataa ccggttcact
    11161 gtccggcact ttttccgttt tacgctcgac ctgcgggcgc gcggtcacac gggttagttc
    11221 agatttcggg cgagctggct gtgttgagac gcgcggctgc gtcggcgtaa tggcatgtaa
    11281 atcttcacta gcgtaagtcg ccagtctggc ctgctgttcg ggcttcgcct gcggcaactg
    11341 actgatcagt tcctgcaact gcggcggcaa cggcgtgagt tcctgctgtt gcgtctggcg
    11401 ttgcggcgcg ttttgcacca ccgctttcgt caattgctgg ataaccggcg atgttgcgac
    11461 gtttcgcggc tgcggcgtgt cctgatgcgg cgcggcaggt tgcggaagta acagcgccat
    11521 tagcgcctgc atcgccaccg gatcgttctc ctgcgggcgc gtcgcctgtt gctgactggt
    11581 ttttgcgcta tgaacgcgct ccgccaccgc cgcgaccgcc attttcggca gggtaaacgc
    11641 gggcgcgttt tcaccgctca ccggcggtaa aatatccgcc ttcaacgaag cggtttccgc
    11701 aagcgttccg agcgtggcaa gcaatgtcgg attcatgact tctccgaaag taaattaaca
    11761 attgagtagg ccagtacgcc ttcctgatgc cgctgatgct gttccatacg cgctttcaac
    11821 acttctgcgc gctgctcgcg ccgtttaatc agggcgttaa acgcgtcata cagtcgcgct
    11881 ttcactgcat ccaactgcgc cgcatcggtt tccgccgccg cgtaatcgtg aaactggcgt
    11941 atcaactgct gatgcagtgc cggaaggcgt cgccagcgtt gcttgtcgag ggcttcattc
    12001 atcgcctcga ccagtgcgaa aagtttcctg cgttgttttt ccatcgtctt aacccagctt
    12061 gcccgaaagc ccttcccagc cttcgcgcag gttgcttaag atcagcatca cttcatcgat
    12121 tttctctgcc gacagttcgc cgctggcctc atacaatcgg taaacgcagt ggtcatagag
    12181 tcgcgaaagg ttgaccacca gctcgccgcc agtttcaaac tccagcgaac tggtgagcgc
    12241 attgagaatg tcgatgcatt tattgatgct ctgggctttc ttctcgaaac gccgcccttc
    12301 aatatggctt ttggcgcgtt ccagttcgtc catcaggcca gaaaacagca ccagcaccag
    12361 ttccagaggc gatgccgccg cagtacgcgc cgcgagatcg atctctttat attgcgaata
    12421 gccgtcctgt acttggtaca tctttaggga tattccttct gagttacgcg aatgccgcca
    12481 tgctggcttt catggtgtac acctcgacca gcgtgttggt gtactcctcg agataacggt
    12541 cgtagttggc gttataggta tcagtaagct gatcgccttc gtcctgaatt ttgctcatct
    12601 gatcgtcaat gttctgctgg cgcagggtga tgatgccgtt gctggagtcc gtgtaggtgt
    12661 taataaggtc gtccatctgc gccaccatgc tgttgtcgcc aacgaaaata gaggtcagac
    12721 cgtcaggatt tttcgccatt tcgtcgttga actggtcgga gtcaatctgt aagtggccgt
    12781 gagaatcgag ggtgatgcca tagtcaacaa tcgacacgcc gttgtagctg gcatgggcga
    12841 tgtcatcgag ctggttcgcc agtgaactga gtccggcgtc gcctgcaaat accccggcgc
    12901 tggtactgtc atcaccgtgg gtggttagcg aatcaaccgt atcaatcaag gtgttatagg
    12961 catcgacaaa ggtctgtact ttctcctggc tggcgctgga atcttcgctg atgttaaagg
    13021 tagtgagatc gctgtcagaa tcgctgactt cggtgaaagt catggtgaca ccgggaatca
    13081 catcatcaaa cgtattgctg ctgttggtga tctcaggccc cgtggcgctg ccaagatgaa
    13141 taatcgcgtc ctgagcggag gaaacatccg tggcaacggg cgcgctggtg ctatcgttgc
    13201 tggcatcatg ccccgttacg ctcaccgaaa acgcgctctg cgccccggtg ctatccgagg
    13261 tgagcataat cgttgtggtg ccgtcggttt ttaccagcgc cgccgacacg cccggattat
    13321 catcggagtc gttaatcgca ttcaccagtt cactggcgtc gataaaaccg tcgccgtcac
    13381 cattctgatc tgccgccgac agatcgatat ccatggtgct gtcgcccatc gtcagttcaa
    13441 aagtgccggt ggcggaaaag gcgtcgtcgc ccatactgaa cgtggtttgc tgcccctgtg
    13501 ccagttgctc aacgaaaaat gagtagcttc ccgcctgcgc ctgggaattg gcggaaacgc
    13561 tcgccgagtc attattgctg gtggcggcaa acgtcaccgg gccgtcggta tcgctgttca
    13621 gcgcgtcaac ggcgctctga aaatcgctca acgctgaact gagcgaatcc aggccgctgc
    13681 tttcagcatc cagctctgtt tgcttctcct gcaagtttgc cgcctgagtg gcgacatccg
    13741 catacgcgat ttcttgcgca atggttcttg ggttgatcat tgatgactcc ggataacgat
    13801 gtcaggttat tggcaaaagg cgtgccaata ttttttatgt gaataatcaa tagattaaaa
    13861 aacaggcgga aatggcgttt ccggtctggc ggaaaaaagc ctgccgcaga aagcacaaca
    13921 ccgcccgcag gcggtgttgt tcgtagtccg aataaggcgt ttacgccgca tccggcaagt
    13981 ccgccattac tgcagcaggc tggaaaccat gctggacatg ctgttggact gtttcagcat
    14041 ggtgatgcca gtctgcatca gcacctgctg tttggtcata ttggacgctt ccgtcgcgta
    14101 gtcggtatcc ataatgttac cgatagcgac ttcggtgttg tcctgcatac tggtcaggtt
    14161 gttggcggtg tcgttcagac ggttgataga cgcacccagc ttggactgga tctgcgaaac
    14221 gtcatccatc gcggtagaga tgctggtgat catctggtta gccgagccag acgcggtcag
    14281 ctcagtaccg ccagaaacac ctgcagtacc cgtggtgtct gcctgatccg cgctaaagga
    14341 gttgctgata gcagaaagat cggtcaccag cgtgttcagt tggctggaga tgttcacggt
    14401 catggtgtcg gaagattccg cgcccacctg gaaagttacc gcgctctgga acagaccgtc
    14461 agtaccggtg ttgctggtac cggaaacacc gaacaggttg gtgccgccgt aagtggtgtt
    14521 ttgcagcatg tcagacatct gctgacccag ttcgtcgtat tcgtcctgca tcgcctgcag
    14581 atcgtcgtcg ctgtaggtgc cgttcgctgc ctgagttgac aggtctttca tacgacccag
    14641 cacatcagac atttcatcga acatactatc ggcggtttgc agcatcgcgg ttgcgtcatt
    14701 gatgttgctc agcgccacgc ccataccgct ggactgtgct gtcagacggt tagcaatttg
    14761 tttgcctgcg gcgtcatcgg cagaagagtt aatccggtta cctgtcgcca ggcgttccat
    14821 agatgtagac aaagaagagc tgcttttgct gattgcatta acggcggcca ttgaggcgtt
    14881 attggtgtta atagataaca tgatactgct ccttgggatt aatcgttttc gtttcgatac
    14941 cctgtaaaaa cgacacctaa ggagcaaact taaaaccgcc gttaaaaaaa tttcactgcg
    15001 atttatctga tgccactttt agctaaaaaa agcagagttt aaattcagat aaaaagatta
    15061 ttagcaatat ctgccacatg gccaggtatc aaaatatgtc tttatcaaac gcttgatata
    15121 gttaagatta ccatcaatta acgcgttttc ttctcaatgt actgatgcca aaatttacat
    15181 tgcgttgcaa atagtacaca tttggctatt gtaccagtcc tgtcatttcg
//
LOCUS       DAZFRJ010000108        15192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000108 DAZFRJ010000000
VERSION     DAZFRJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.109"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            202..537
                     /locus_tag="ACHKUN_003010"
     CDS             202..537
                     /locus_tag="ACHKUN_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO6661227.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            697..1140
                     /gene="yjdN"
                     /locus_tag="ACHKUN_003011"
     CDS             697..1140
                     /gene="yjdN"
                     /locus_tag="ACHKUN_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO6661228.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1273..2061
                     /gene="phnC"
                     /locus_tag="ACHKUN_003012"
     CDS             1273..2061
                     /gene="phnC"
                     /locus_tag="ACHKUN_003012"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO6661229.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2086..3102
                     /gene="phnD"
                     /locus_tag="ACHKUN_003013"
     CDS             2086..3102
                     /gene="phnD"
                     /locus_tag="ACHKUN_003013"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6661230.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3208..3987
                     /gene="phnE"
                     /locus_tag="ACHKUN_003014"
     CDS             3208..3987
                     /gene="phnE"
                     /locus_tag="ACHKUN_003014"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO6661231.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4008..4733
                     /gene="phnF"
                     /locus_tag="ACHKUN_003015"
     CDS             4008..4733
                     /gene="phnF"
                     /locus_tag="ACHKUN_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO6661232.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4734..5186
                     /gene="phnG"
                     /locus_tag="ACHKUN_003016"
     CDS             4734..5186
                     /gene="phnG"
                     /locus_tag="ACHKUN_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO6661233.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5183..5767
                     /gene="phnH"
                     /locus_tag="ACHKUN_003017"
     CDS             5183..5767
                     /gene="phnH"
                     /locus_tag="ACHKUN_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO6661234.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5767..6831
                     /gene="phnI"
                     /locus_tag="ACHKUN_003018"
     CDS             5767..6831
                     /gene="phnI"
                     /locus_tag="ACHKUN_003018"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO6661235.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6824..7669
                     /gene="phnJ"
                     /locus_tag="ACHKUN_003019"
     CDS             6824..7669
                     /gene="phnJ"
                     /locus_tag="ACHKUN_003019"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO6661236.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7666..8424
                     /gene="phnK"
                     /locus_tag="ACHKUN_003020"
     CDS             7666..8424
                     /gene="phnK"
                     /locus_tag="ACHKUN_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO6661237.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMGEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8535..9215
                     /gene="phnL"
                     /locus_tag="ACHKUN_003021"
     CDS             8535..9215
                     /gene="phnL"
                     /locus_tag="ACHKUN_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO6661238.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9212..10348
                     /gene="phnM"
                     /locus_tag="ACHKUN_003022"
     CDS             9212..10348
                     /gene="phnM"
                     /locus_tag="ACHKUN_003022"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO6661239.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10348..10905
                     /gene="phnN"
                     /locus_tag="ACHKUN_003023"
     CDS             10348..10905
                     /gene="phnN"
                     /locus_tag="ACHKUN_003023"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO6661240.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10892..11326
                     /gene="phnO"
                     /locus_tag="ACHKUN_003024"
     CDS             10892..11326
                     /gene="phnO"
                     /locus_tag="ACHKUN_003024"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO6661241.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11328..12086
                     /gene="phnP"
                     /locus_tag="ACHKUN_003025"
     CDS             11328..12086
                     /gene="phnP"
                     /locus_tag="ACHKUN_003025"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO6661242.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12233..12562)
                     /gene="yjdP"
                     /locus_tag="ACHKUN_003026"
     CDS             complement(12233..12562)
                     /gene="yjdP"
                     /locus_tag="ACHKUN_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO6661243.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12631..12948)
                     /locus_tag="ACHKUN_003027"
     CDS             complement(12631..12948)
                     /locus_tag="ACHKUN_003027"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO6661244.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13201..15186
                     /gene="yjcS"
                     /locus_tag="ACHKUN_003028"
     CDS             13201..15186
                     /gene="yjcS"
                     /locus_tag="ACHKUN_003028"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO6661245.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3441 a         4497 c         4182 g         3072 t
ORIGIN      
        1 agagcattaa cattatctta aataataaat ggaggcaaaa agattatttt ctgtttgcgt
       61 ttcctttcaa atgaaaacga tcgtcgtcta aaatcagcag tacccccgac aaactcaggg
      121 attttgtgta taattgcggc ctttttcggc aatctgccgt tttttggcgc ttttgccctg
      181 ctgacttttg aggaaatcca catgtcatta ccacactgcc caaaatgcaa ctccgaatac
      241 acttacgaag ataacggcat gtacatctgc ccggaatgtg cctacgaatg gaacgacgca
      301 gaacctgcac aggaaagcga cgagctgatc gttaaagatg ctaacggcaa tctgctggct
      361 gacggcgaca gcgttaccat cattaaagat ctgaaggtga aaggtagctc ttcgatgctg
      421 aaaattggca ccaaagtgaa aaacatccgc ctggttgaag gcgaccataa catcgattgc
      481 aaaatcgacg gttttggtcc gatgaaactg aaatctgagt ttgtgaaaaa gaactgattg
      541 tattgtgatc ggtaagccgg ataaggcgct cgcgccgcat ccggcaacga tgccgactgc
      601 ctgatgcgac gctgaccgcg tcttatcagg cttccctctc atctgtataa atttcgaact
      661 acacttaact ggcttctctt aactgaggtc accatcatgt cgttaagtcc ctacctctct
      721 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
      781 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
      841 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaacgtgcg cattgccgga
      901 agcgacatca tgatgagcga tgctataccg tcaggaaaag ccagctactc cggctttacg
      961 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
     1021 aacggaaaaa tcgaaatggc ctggcaggaa actttctggg cgcatggctt tggcaaagtc
     1081 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
     1141 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
     1201 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
     1261 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
     1321 catcaggcgc tgcatgcggt tgatctgaac attcatcacg gtgaaatggt ggctctgctt
     1381 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
     1441 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
     1501 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
     1561 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
     1621 ttctggcgca cctgttttag ctggttcacc ggcgagcaga aacagcgcgc gttacaggcg
     1681 ctgacccgcg ttggcatggt gcattttgcc catcagcgcg tttccaccct ctccggcgga
     1741 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
     1801 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
     1861 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
     1921 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
     1981 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
     2041 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
     2101 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
     2161 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
     2221 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
     2281 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
     2341 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
     2401 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
     2461 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
     2521 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
     2581 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgctgg gcatgaaacc
     2641 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
     2701 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
     2761 ccgctgatcc caggcgatcc gatcgtctgg cgtaaaaacc tttccgaaac caccaaagac
     2821 aagatctacg acttctttat gaactacggc aaaacgccgg aagaaaaagc ggtgctggaa
     2881 cgcctgggct gggcgccgtt ccgcgcttcc agcgacctgc aactggtgcc gattcgccag
     2941 ctcgcgctgt ttaaagagat gcagggcgtg aaaagcaata aaggactgaa tgagcaggac
     3001 aagctggcaa aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
     3061 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aaatcgtagg tcggataaga
     3121 cgccccggcg tcgcatccga caatgtgcag gcgttgatgc cggatgcggt gcaagcacct
     3181 tatccggcct acagaccgga gccaaacatg caaaccatca ccatcgcccc acccaagcgc
     3241 agctggttct cgcttctgag ctgggccgtt gttctcgccg tgttggtcgt ctcgtggcag
     3301 ggcgcggaaa tggccccgct cacgctgatt aaagacggcg gcaacatggc gacgttcgcc
     3361 gccgacttct tcccgcccga tttcagccag tggcaggatt acctcaccga aatggccgtc
     3421 acgctgcaaa tcgccgtctg gggcacggcg ctggcggtgg ttctctccat ccccttcggc
     3481 ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc agcccgttcg ccgcctgatg
     3541 gacgcctgcc gcgtcattaa cgaaatggtc ttcgccatgc tgttcgtggt cgccgtcggc
     3601 ctcggcccgt tcgctggcgt gctggcgctg tttatccaca ccaccggcgt gctctccaaa
     3661 ctgctttccg aagcggtgga agcgattgaa cccggcccgg tggaaggcat tcgcgccacc
     3721 ggcgccaaca agctcgaaga gatcctctac ggcgtgctgc cgcaggtgat gccgctgctg
     3781 atctcctact ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt cgtcggcatg
     3841 gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga ttcgcggttt ccagttccaa
     3901 caaacctgcg ccctgatggt gcttatcatc gtcacggtca gcctgctgga tttcctctct
     3961 caacggttgc gtaagcactt tatctgataa gcgaggcatt gatatctatg cacttgtcta
     4021 cacatccgac cagctaccca acacgctatc aggagatagc cgcaaaactt gagcaggagc
     4081 ttcgtcaaca ctaccgctgc ggcgactatc ttcccgccga gcagcaactg gcggcgcgct
     4141 ttgaggtgaa ccgccacacc ctgcgccgcg ccatcgacca actggtggaa aaaggctggg
     4201 tacagcgccg tcagggcgtc ggcgtgctgg tgctgatgcg cccgttcgat tacccgctca
     4261 acgcccaggc gcgttttagc cagaacctgc tggaccaggg tagccatccc accagcgaaa
     4321 aactgctgtc ggtattgcgc cccgcgtccg gtcacgtcgc tgacgcgctg gggattaccg
     4381 agggcgagaa cgttatccat ttgcgcaccc tgcgtcgcgt caacggcgtc gcgctctgtt
     4441 taatcgacca ctactttgcg gacctcaccc tttggccgac gctgcaacgc ttcgacagcg
     4501 gttcgctgca cgattttctg cgcgagcaaa ccggaattgc gctgcgccgc agccagacgc
     4561 ggatcagcgc ccgtcgcgcc caggccaaag agtgccagcg tcttgaaatt ccgaatatgt
     4621 cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc ccggcggagt
     4681 actccgtcag cctgacgcgc gccgacatga ttgaattcac tatggagcac tgaatgcacg
     4741 cagataccgc gacccgccag cactggatgt ccgtactggc gcacagccaa ccggctgaac
     4801 tggcagcccg cctgaaagca ctgaacatca ccgccgacta tgaggttatc cgcgccgccg
     4861 aaaccggcct ggtgcagatt caggcgcgga tgggcggcac cggcgaacgc ttttttgccg
     4921 gcgacgccac gctcacccgc gccgccgtac gcctgactga cggcacgctc ggctacagct
     4981 gggtgctggg gcgcgacaaa cagcacgccg aacgctgcgc gctgattgac gcgctgatgc
     5041 agcaatctcg ctattttcaa aacttatcag aaacccttat tgccccgctg gacgctgacc
     5101 gtatggcacg cattgccgca cgccaggccg aagtgaacgc cagccgggtc gacttcttta
     5161 cgatggttcg cggagacaac gcatgaccct ggaaaccgct tttatgcttc ccgtgcagga
     5221 tgcccagcac agttttcgtc gcctgttaaa ggccatgagc gagccgggcg tgattgtcgc
     5281 cctgcatcag ctcaagcgcg gctggcagcc gctgaatatc gccaccacca gcgtgctgct
     5341 gacgctggcc gataacgaca cgccggtgtg gcttgccgcg ccattgagta acgatatcgt
     5401 cagccagagc ctgcgttttc ataccaacgc gccgctggtc agccagccgg aacaggcgac
     5461 cttcgcggtg acggatgagg cgatttccag cgaacagctt aacgcccttt ccaccggcac
     5521 cgccgttgcg ccggaagcgg gtgcaacgct gattttacag gtcgccagcc tgagcggcgg
     5581 gcgcatgtta cgtctcaccg gcgcgggcat tgccgaagaa cgaatgatcg ctccgcagct
     5641 gccggagtgc attctgcacg aactcaccga gcgcccgcac ccgttcccgc tcggcatcga
     5701 cctgatcctg acctgcggcg agcgcctgct ggctattccg cgaaccactc atgtggaggt
     5761 gtgctgatgt acgttgccgt gaaagggggc gagaaggcga tcgacgccgc tcacgccctg
     5821 caagagagcc gacgccgggg cgataccgat ttgcctgaac tgagcgtcgc ccagattgaa
     5881 cagcagctta atcttgcggt ggatcgcgtg atgaccgaag gcggcattgc cgaccgcgaa
     5941 ctggcggcgc tggcgctgaa acaggccagc ggcgataacg tcgaagcaat tttcctgctg
     6001 cgcgcctacc gcaccacgct ggcgaagctc gcggtaagcg agccgctcga caccacagag
     6061 atgcgtctcg aacggcgtat ttcggcggtt tataaagaca ttcccggcgg ccagctgctt
     6121 ggcccaacct acgactacac ccatcgcctg ctcgatttca ccctgctggc aaacggtgaa
     6181 gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt cgccgcacgt tttcagcctg
     6241 ctggcgcgtc aggggctggt gaagtttgaa gaagatagcg gcgcacagcc ggatgacatc
     6301 acccgcacgc cgccggttta cccctgctca cgctcctccc gcctgcaaca attaatgcgc
     6361 ggggacgaag gctatttgct ggcgctggcc tactccaccc agcgtggtta cgggcgcaat
     6421 cacccgttcg cgggcgagat ccgcagtggt tacatcgacg tgtcgattgt gccggaagag
     6481 ctgggatttg cggtaaacgt cggcgaacta ctgatgaccg agtgtgaaat ggtcaacggt
     6541 tttatcgacc cgccggatga gccgccgcac ttcacgcgcg gctacgggct ggtattcggc
     6601 atgagcgagc gcaaagcgat ggcaatggcg ctggtcgatc gtgcgttgca ggctccggaa
     6661 tacggcgagc acgcgacagg cccggcgcag gatgaagagt ttgtgctggc acatgccgac
     6721 aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cccactacgt cgatttccag
     6781 gccgaactgg agctactcaa acgtctgcaa caggagaaga accatggcta atctgagcgg
     6841 ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg ctatcttaaa
     6901 agcggtggcg atccccggtt atcaggtgcc gtttggcggg cgcgagatgc cgatgccata
     6961 cggctgggga accggcggca tacagctcac cgccagcgtg attggcgaaa gcgacgtgct
     7021 gaaggtgatt gaccagggtg cggatgacac caccaacgcc gtgtcgattc gcaacttctt
     7081 taagcgcgtg accggggtaa acaccactga acgtacggac gatgcgacgc ttatccagac
     7141 gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag atcattatct tccaggtgcc
     7201 aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg gaaacccgca ccatgcacgc
     7261 gctggaagag tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc gcttcggtca
     7321 tatcgccact acctatgcct atccggtgaa ggtgaacggg cgctacgtaa tggacccgtc
     7381 gccgatcccg aaattcgata acccaaaaat ggacatgatg cccgccctgc aactgttcgg
     7441 cgcggggcgc gagaagcgca tctatgcggt gccgccgttt acccgcgtgg aaagtctcga
     7501 tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca tctgcggatc
     7561 gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca tgtttgtctg
     7621 ctccgatacc gattattgcc gccaacagag cgaggcaaaa aaccaatgaa tcaaccgtta
     7681 ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag cgatgtctct
     7741 tttgatttat ggccggggga agtgctgggc attgtcgggg agtccggctc cgggaagacc
     7801 acgctgctga aatcaatctc cgcgcgcctg acgccgcagc agggggagat tcactatgag
     7861 agccgttcgc tgtacgcaat gggcgaggcc gaccgccgtc gcctgctgcg caccgaatgg
     7921 ggcgtagtgc atcagcatcc actcgacggc ctgcgccgtc aggtctcggc aggcggcaat
     7981 atcggcgagc ggctgatggc gaccggggca cgtcattacg gcgatattcg tgccaccgcg
     8041 cagaagtggc tggaagaggt ggaaattccc gccaaccgga tcgacgacct gccgaccacc
     8101 ttttccggcg gcatgcagca acgtttgcag atcgcccgca acctggtgac gcatccgaag
     8161 ctggtgttta tggacgagcc gaccggcgga ctggatgtgt cggtacaggc acgcctgctc
     8221 gacctgctgc gcggcctggt ggtggagctg aacctcgcgg tagtgattgt cacccacgat
     8281 ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg gcaagtggtg
     8341 gagagtgggt taaccgaccg cgtgctcgac gacccgcacc atccgtatac acaactgctg
     8401 gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc cttatccggc
     8461 ctacaaatgc gctccccgta ggtcggataa gacgcgtcag cgtcgcatcc gacacccgaa
     8521 ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa aaccttcatc ctgcaccagc
     8581 aaaacggcgt gcgcctgccc gtcttacaac gtgcctcgct caccgtcaac gcgggcgaat
     8641 gcgtggtgct ccacggccat tccggcagcg gcaaatcaac cttgctgcgc tcgctgtacg
     8701 ccaactatct gcccgacgaa gggcaaatcc agatcaaaca cggtgacgaa tgggtcgatc
     8761 tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa aaccaccgtc ggctgggtga
     8821 gccagtttct gcgcgtcatc ccgcgtattt cagcactgga agtggtgatg cagccgctgc
     8881 tcgataccgg cgttccgcgt gaagcctgcg ccgctaaagc cgcgcgtctg ctcacccgcc
     8941 tgaacgtgcc ggaacgcctg tggcacctgg cgccatcaac cttttccggt ggcgaacagc
     9001 agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc cattctgctg ctcgacgaac
     9061 ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt cgagctgatt cgcgaagcca
     9121 aaacccgtgg cgcggccatc gtcggcatct tccatgacga agccgtacgt aatgacgtcg
     9181 ccgaccgctt gcacccaatg ggagcctctg catgattatc aataacgtca aactggtgct
     9241 ggaaaacgaa gtggtacacg gttcgctgga ggtgcaggat ggcgaaatcc gcgcctttgc
     9301 cgaaagccag agccgcctgc cagaagcgat ggacggcgaa ggtggctggc tactacccgg
     9361 cctgattgag ctgcataccg acaacctcga taaattcttc accccgcgcc cgaaagtcga
     9421 ctggcccgcc cattcggcga tgagcagcca cgacgcgctg atggtggcaa gcggcatcac
     9481 caccgtactg gacgccgtgg cgattggcga cgtgcgcgac ggcggcgatc ggctggagaa
     9541 tctggagaag atgatcaacg ccatcgaaga gacgcaaaaa cgcggcgtca accgcgccga
     9601 gcaccgcctg cacctgcgct gcgaactgcc gcatcacacc acactgccgc tgtttgaaaa
     9661 actggttcag cgcgaaccgg tgacgctggt gtcgctgatg gaccactcac cgggccagcg
     9721 ccagttcgct aaccgcgaga agtatcgcga atattatcag ggcaaatact ccctcaccga
     9781 tgcgcagatg cagcagtacg aagaagagca attggcgctc gccgcacgct ggtcgcagcc
     9841 gaatcgcgaa tccatcgccg ccctgtgcca cgcgcgacaa attgcgctcg ccagccacga
     9901 tgacgccacg cacgcccacg ttgccgaatc ccaccaactt ggcagcgtga tcgccgaatt
     9961 tcccaccacg ttcgaagcgg cggaagcctc gcgtaagcat ggcatgaacg tgctgatggg
    10021 tgcgccaaat atcgtgcgtg gcggctcgca ctccggcaat gtggcggcca gtgaactggc
    10081 gcagcttggt ctgctggata tcctctcttc cgactactac cccgccagcc tgctggatgc
    10141 ggcgttccgc gtcgccgatg acgagagcaa ccgctttacg ctaccgcagg cggtgaggct
    10201 ggtgaccaaa aatccggcgc aggcgctgaa tcttcaggat cgcggggtga ttggcgaggg
    10261 taaacgcgct gacctggtgc tggcgcatcg ccagggcaat cacattcata tcgaccacgt
    10321 ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt aatggggccg
    10381 tccggctccg ggaaagacag cctgctggcg gaactccgcc tgcgggaaca aacgcagtta
    10441 ctggtggcgc atcgctacat cacgcgcgac gccagcgccg gaagtgaaaa ccatatcgcc
    10501 ctgagtgagc gggagttttt tacccgcgcg gggcaaaacc tgctggcctt aagctggcac
    10561 gctaacggtc tgtattatgg cgtcggcatc gagattgacc tctggctgca cgctggattc
    10621 gacgtagtcg tcaacggttc acgcgcgcat ttgccgcagg cacgggcgcg ctatcaatcg
    10681 gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc tgcgccagcg cctggaaaac
    10741 cgtggccgtg aaaacgccag tgaaattaac gcccggctgg cgcgcgccgc ccgctatact
    10801 ccacaggatt gccatacgct caacaatgac ggcagcctgc gccagtcggt cgacacactg
    10861 ctgacgctga ttcatcagaa ggagaaacac catgcctgct tgtgagcttc gcccggccac
    10921 gcagtacgat accgacgcgg tttacgcgct gatttgtgag ctaaaacagg cggagtttga
    10981 ccaccacgcg tttcgcgtgg gctttaacgc caacctgcgc gacccgaaca tgcgctacca
    11041 tctggcgctg ctggatggcg aagttgtcgg catgatcggc ctgcatttgc agtttcatct
    11101 gcatcatgtc aactggatcg gcgaaattca ggaactggtg gtgatgccgc aggcgcgcgg
    11161 cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa gaagcccgcc aggccggggc
    11221 cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac gcgcaccgtt tctatctgcg
    11281 agaaggctac gagcagagcc acttccgctt caccaaggcg ctgtaacatg agcctgaccc
    11341 tcacgctcac cggcactggc ggcgcacagg gcgttccggc atggggctgc gagtgcgcgg
    11401 cctgcgccag agcgcggcgc tcgccgcagt atcgccgcca gccatgcagc ggcgtagtga
    11461 agtttaacga cgcgatcacc ctgatcgacg ccgggcggca cgatctcacc gatcgctggt
    11521 cgcctggatc gttccagcag tttttgctga cgcactacca tatggatcac gtccaggggc
    11581 tatttccgct acgctggggc gtgggcgatg tgatcccggt ctacggcccg ccggatgaac
    11641 agggctgcga cgatctgttt aaacatccgg gcctgcttga tttcagccac acagtggaac
    11701 cgtttgtggt gtttgatttg caggggttgc aggtcacgcc gctgccgctt aaccactcaa
    11761 aactgacctt cggttatctg ctggaaacgg cacatagccg ggtggcgtgg ctgtctgaca
    11821 ccgctggcct gccggaaaaa acgctgaaat ttttactcaa taatcatccg caggtgatgg
    11881 tgattgattg cagtcacccg ccgcgcgcgg atgcaccgcg taatcactgt gatttaaata
    11941 ccgtgcttgc gctgaatcag gttatccgct cgccacaggt gattctgacc catatcagcc
    12001 accagtttga tgcgtggctg atggaaaacg cactaccgtc agggtttgag gtggggtttg
    12061 atgggatgga gattggggtg gcgtgatgag agggaatgtg cgcgctggcc ccctcaccct
    12121 aaccctctcc ccagaggggc gaggggaccg attgtgctca atattgaata ttgcgctcgt
    12181 tttctccctc tccccattgg ggtgaggggc gatgcctgct ccatacccaa cctcatcgcc
    12241 catactcatc ttccattctc cgctcttcat cctccagttg ccgacgctcc tgatcaagct
    12301 ggcgctggcg atcgtccagc tgcctgcggc gatcttcaaa ctggcggcgg cggtcgtcat
    12361 attgtctgcg ccgatcgtcg ctcacttcac gctgccagcc gtcgtcgcgc gaatcttcat
    12421 agtctcgccc acggtcaggg ttataagcgt cattaatcgc ctgctgaata ttaccaatag
    12481 tatcgtcgat aatatcagcc tgggccggaa cggtggacag cgtgagcagg gtgaataaaa
    12541 gaaatagcgg aaagcgtttc attagccaac ctcaaaaaga aactctatcc acattagtca
    12601 ttactcatcc atgcaagtag tggatgaatc tcaatttctc cgctgctcta ttgccgtaat
    12661 cgcctccacg cgttgttgat gacgaccgcc ttcgtactgt gcgcccagcc acgcatccac
    12721 aatcattttt gccagttcga ggccaaccac tcgtgaacca aaagccagtg caacttcact
    12781 ggcataatgc ggataatcag tgcgctctga cgacctggtg tctttatcaa tcacctcact
    12841 cattgaccat gaataaaagg ctggcacagc aacacagaat cctgcggtgc cagcgacatg
    12901 accgcccatt gctccggtac gttttttaaa aagaaacacc ggttgcattg ataaattgtg
    12961 ctcagtattt gccgaagtac aatatatata ataaatatat aaaacacctc atattattta
    13021 cagagataat aaattcgcgt aattttcccc cttcagcaca aatgcctaat aaaatggatc
    13081 gttccgctca cacttccact tattaaagtc gcaaattccc aattatgata acgccagctt
    13141 taaatgacgt attctgcatg gattacatat ttacgacaca aatgataaat ggagatttat
    13201 atgaataact ctcggttatt ccgtttgagc aggattgtta ttgcgttaac tgctgccagc
    13261 ggcatgatgg taaataccgc taacgcgaaa gaggaagcga aagccgccac tcaatatacc
    13321 caacaggtta atcagaatta cgccaaatca ttaccgttta gcgatcgtca ggattttgac
    13381 gacgctcagc gtggatttat cgctccgctg ctggatgaag gtattctgcg cgatgctaat
    13441 ggcaaaccat attatcgcgg ggaagattat aaatttgata tcaatgcgcc cgcgcccgaa
    13501 accgttaacc ctagcctgtg gcgtcagtcg cagttaaacg gtatttccgg cctgtttaaa
    13561 gtcaccgaca gaatgtacca ggttcgcggt caggatatct ccaacatcac ctttatcgaa
    13621 ggtgataccg ggattattgt catcgacccg ctggtgacgc caccgagcgc aaaagccgcc
    13681 cttgaccttt acttccaaaa tcgcccgcaa aaaccgattg ttgcggttat ttatacccac
    13741 agccataccg accactatgg cggcgtgaaa ggcattatct ctgaagctga tgtgaaatcc
    13801 ggtaaagtgc aggttatcgc ccctgctggc tttatggacg aagccatcag cgaaaacgta
    13861 ctggcgggta atattatgag ccgccgtgca ctttactcct acggcctgct gctggcgcat
    13921 aaccctcagg gtaacatcgg caatggtctt ggcgtaacgc tggcatcggg ctacccgagc
    13981 atcatcgcac cgaacaaaac catcaccaaa accggtgaga agatgattat cgacggcctg
    14041 gagtttgact tcctgatgac cccaggtagc gaagcaccag ccgaaatgca cttctatatt
    14101 ccggccctga aagcgctgtg taccgccgag aacgccacgc ataccctgca caacttctac
    14161 actctacgcg gcgcgaaaac ccgcgacacc agcaagtgga ccgagtatct gaacgaaacg
    14221 ctggatatgt ggggtaacga cgcggaagtc ctgtttatgc cgcacacctg gccggtctgg
    14281 ggcaataagc atatcaatga ttatattggt aaatatcgcg atactatcaa gtacattcac
    14341 gaccagaccc tgcacctggc gaaccagggc tacaccatga atgaaatcgg cgacatgatt
    14401 aaactgccgc ctgcacttgc caataactgg gccagccgtg gctattacgg ttctgtcagc
    14461 cacaacgccc gcgcggtgta taacttctat cttggctatt acgacggtaa cccggctaac
    14521 ctgcatccgt atggtcaggt ggagatgggt aaacgttacg tgcaggcgct gggcggttct
    14581 gcccgtgtca tcaacctggc gcaagaagcg aacaagcaag gtgattaccg ctggtcggca
    14641 gaactgctga aacaggtgat tgccgccaac ccgggtgacc aggtcgcgaa gaatctgcaa
    14701 gcgaataact ttgaacagct gggctatcag gccgagtccg ccacctggcg cggtttctac
    14761 ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt tcagccacgg caccaccggt
    14821 tccccggaca ccattcgcgg gatgtcggtc gaaatgctgt tcgactttat gtccgttcgc
    14881 ctcgatagcg cgaaagccgc gggtaaaaat atcagcctga acttcaatat gagcaatggc
    14941 gataacctca acctgacgct gaacgatagc gtgcttaact accgtaaaac actgcaatcc
    15001 caagctgacg cctctttcta catcagccgt gaagatctgc acgccgtgct gaccgggcaa
    15061 gccaaaatgg cggatctggt aaaagcgaag aaagccaaaa ttattggcaa tggcgcgaaa
    15121 ctggaagaaa ttatcgcctg tctggataat ttcgatttgt gggtgaatat cgtaacccca
    15181 aattaatgtc ac
//
LOCUS       DAZFRJ010000109        15184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000109 DAZFRJ010000000
VERSION     DAZFRJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.110"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            89..1066
                     /gene="ybeQ"
                     /locus_tag="ACHKUN_003029"
     CDS             89..1066
                     /gene="ybeQ"
                     /locus_tag="ACHKUN_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO6661246.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1136..1618)
                     /gene="ybeL"
                     /locus_tag="ACHKUN_003030"
     CDS             complement(1136..1618)
                     /gene="ybeL"
                     /locus_tag="ACHKUN_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO6661247.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1853..4435
                     /gene="leuS"
                     /locus_tag="ACHKUN_003031"
     CDS             1853..4435
                     /gene="leuS"
                     /locus_tag="ACHKUN_003031"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO6661248.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4450..5031
                     /gene="lptE"
                     /locus_tag="ACHKUN_003032"
     CDS             4450..5031
                     /gene="lptE"
                     /locus_tag="ACHKUN_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO6661249.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5031..6062
                     /gene="holA"
                     /locus_tag="ACHKUN_003033"
     CDS             5031..6062
                     /gene="holA"
                     /locus_tag="ACHKUN_003033"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO6661250.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6064..6705
                     /gene="nadD"
                     /locus_tag="ACHKUN_003034"
     CDS             6064..6705
                     /gene="nadD"
                     /locus_tag="ACHKUN_003034"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO6661251.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6729..7340
                     /gene="cobC"
                     /locus_tag="ACHKUN_003035"
     CDS             6729..7340
                     /gene="cobC"
                     /locus_tag="ACHKUN_003035"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO6661252.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7600..7917
                     /gene="rsfS"
                     /locus_tag="ACHKUN_003036"
     CDS             7600..7917
                     /gene="rsfS"
                     /locus_tag="ACHKUN_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO6661253.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7921..8388
                     /gene="rlmH"
                     /locus_tag="ACHKUN_003037"
     CDS             7921..8388
                     /gene="rlmH"
                     /locus_tag="ACHKUN_003037"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO6661254.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8419..10320
                     /gene="mrdA"
                     /locus_tag="ACHKUN_003038"
     CDS             8419..10320
                     /gene="mrdA"
                     /locus_tag="ACHKUN_003038"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO6661255.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10323..11435
                     /gene="mrdB"
                     /locus_tag="ACHKUN_003039"
                     /gene_synonym="rodA"
     CDS             10323..11435
                     /gene="mrdB"
                     /locus_tag="ACHKUN_003039"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO6661256.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11446..12534
                     /gene="rlpA"
                     /locus_tag="ACHKUN_003040"
     CDS             11446..12534
                     /gene="rlpA"
                     /locus_tag="ACHKUN_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO6661257.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12674..13885
                     /gene="dacA"
                     /locus_tag="ACHKUN_003041"
     CDS             12674..13885
                     /gene="dacA"
                     /locus_tag="ACHKUN_003041"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO6661258.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13996..14259
                     /gene="ybeD"
                     /locus_tag="ACHKUN_003042"
     CDS             13996..14259
                     /gene="ybeD"
                     /locus_tag="ACHKUN_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO6661259.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14360..15001
                     /gene="lipB"
                     /locus_tag="ACHKUN_003043"
     CDS             14360..15001
                     /gene="lipB"
                     /locus_tag="ACHKUN_003043"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO6661260.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3769 a         3851 c         3954 g         3610 t
ORIGIN      
        1 cgacataata cgcataaaat aaggcttacg caaaaatgga acacataaaa atataatatg
       61 ttttatctgc aaccataagg aaatgataat gattttcacg tcaagttgct gcgataattt
      121 atcaatagat gagattatcg aacgtgctga aaaaggcgat tgcgaggctc aatatattgt
      181 tgggttttat tataatcgcg atagcgcaat tgattctccg gacgacgaaa aagcctttta
      241 ctggctgaag ctggccgctg agcaaggtca ttgtgaagca cagtattcct tagggcaaaa
      301 gtataccgag gataaaagcc gtcataaaga taatgagcaa gccatcttct ggctgaaaaa
      361 agctgcccta caaggccata ctttcgcttc caacgccctt ggctggacac tggatcgtgg
      421 agaagccccc aattataaag aagcggttgt ctggtatcag atagccgcgg agagcggaat
      481 gtcttatgcg caaaataatc ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga
      541 ctatgcgctg gcatttttct ggtacaaaca agctgcatta caaggccata gtgacgcgca
      601 aaacaatctg gccgatcttt atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc
      661 cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc
      721 atgggattat aacgctggcg aaggggtgga ccaggactat aagcaagcga tgtactggta
      781 tctgaaggct gccgctcagg aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa
      841 acacggacaa ggcgttgaga aagattatca ggctgccttt gaatggttta cgaaagccgc
      901 tgaatgcaat gacgccactg cctggtataa cctggccatt atgtatcact acggagaagg
      961 aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt catccggaac
     1021 cagagatgtc agtcaggaaa ttcgtgagac tgaagattta ctgtagagat aaaaacaagg
     1081 gccggggaat ttcattcggc ccatattctc tttattgctg tgcgaaatca gactattacg
     1141 gctcaaacgg gcgtctctgg aactggtcat gaccacattt cgggcatagc gtcagcactt
     1201 ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccga
     1261 ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaact tcgcgccatt
     1321 caagctgcgt tttatcggtg atatctgcca gctcctgcca taagctttct ttaatcaccc
     1381 gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt
     1441 ccaggtcacg tcttacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg
     1501 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc
     1561 gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
     1621 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
     1681 taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc
     1741 gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa
     1801 ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
     1861 gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg agaagcgcac
     1921 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
     1981 tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg acgtgatcgc
     2041 ccgctaccag cgtatgctgg gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg
     2101 tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga
     2161 caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg actggagccg
     2221 cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct tcaccgagct
     2281 gtataaaaaa ggcctggtat ataagaagac ttctgcggtc aactggtgcc cgaacgacca
     2341 gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg ataccaaagt
     2401 tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg acgagctgct
     2461 caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc agcgtaactg
     2521 gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt aacgactatg acaacacgct
     2581 gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg cggtagctgc
     2641 gggtcatccg ctggcgcaga aagcggcgga aaataatcct gaactggcgg cctttattga
     2701 cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt
     2761 cgatactggc tttaaagctg ttcacccatt aacgggcgaa gaaattcccg tttgggcagc
     2821 aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg gtcacgacca
     2881 gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccagtta tcctggctgc
     2941 tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg tgctgttcaa
     3001 ctctggcgaa ttcaacggtc ttgaccatga agcggccttc aacgccatcg ccgataaact
     3061 gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc
     3121 tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg gtaccgtaat
     3181 gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga tggacggcat
     3241 taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg gtatgccagc
     3301 gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact atgcgcgcta
     3361 cacttgccca gagtacaaag aaggtatgct ggattccgaa gcggctaact actggctgcc
     3421 ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct acttccgctt
     3481 cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag cgaaacagtt
     3541 gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa acggcgaacg
     3601 taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa
     3661 agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa tgtccaagtc
     3721 gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg
     3781 tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg aatccggtgt
     3841 ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa
     3901 aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg
     3961 cgatgtgcat aaaacgatcg ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa
     4021 caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac caaccgatgg
     4081 cgagcaggat cgcgctctga tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc
     4141 gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga
     4201 caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt
     4261 ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtt ccggtggacg caacggaaga
     4321 acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg atggcgttac
     4381 cgtgcgtaaa gtgatttacg taccaggtaa actcctcaat ctggtcgttg gctaagcgcg
     4441 ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggctgtgtt aatcaccgcc
     4501 gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa ggtcatgatc
     4561 ctggactcag gcgatccgaa cgggccatta agccgtgcgg tgcgtaacca gttacgtctg
     4621 aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc cctgcgtttg
     4681 ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca aacagcagag
     4741 tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga tatctacccg
     4801 attagtgcca aagtcttccg ttcgttcttt gataacccgc aaatggcgtt agcgaaagat
     4861 aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt
     4921 aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac gtcgaccaca
     4981 acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg atgattcggt
     5041 tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
     5101 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg
     5161 cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga
     5221 tcttttcgtt atgccaggct atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt
     5281 taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc accggacttc
     5341 tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg
     5401 ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc
     5461 aggctcagct tccccgctgg gttgctgcgc gcgcaaaaca gctcaactta gaactggatg
     5521 acgctgcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg
     5581 cactggagcg tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac
     5641 aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct ttgttgatgg
     5701 gaaaaagtaa gcgcgcgttg catattcttc agcaactgcg tctggaaggc agcgaaccgg
     5761 ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg aaacgccagt
     5821 ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag aaccgccggg
     5881 gcatgatggg cgaggcgtta aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac
     5941 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc
     6001 tggaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt
     6061 gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca ctatggtcat
     6121 cttaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac aatcatccct
     6181 aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg taaacacatg
     6241 cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga gctaaagcgc
     6301 aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca agggccggac
     6361 gtgccgctgg cgtttattat tggtcaggat tcattgctga cctttccgac ctggtacgaa
     6421 tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg ttacccactt
     6481 gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca taacccggaa
     6541 gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg gtttaacatc
     6601 tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga tttattgccg
     6661 gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct gtcgttacgg
     6721 agggcatgat gcgactgtgg ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc
     6781 tttacagcgg tcatgcgccc acccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc
     6841 tgcatacact gctagatgat gtttcctttg acctggtttt atgcagtgaa ctggaacggg
     6901 cacagcatac cgcgcgacgg gttctcagtg accgccagct ccccgtgcaa atcatccctg
     6961 aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag
     7021 aagatgccga aaactatagc gcgtggtgca atgactggca gcatgctatc cccacgaacg
     7081 gtgaaggatt tcaggcattt tcgcaacgtg tggaaagatt tatcgcaagg cttagtgaat
     7141 atcagcacta tcagaatatt ttaatcgtca gccatcaggg cgtactgagt ctgttaattg
     7201 ctcgtctaat tggcatgcct gccgaatcta tgtggcattt tcgcgttgac caggggtgtt
     7261 ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca
     7321 tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga
     7381 cagccccggg caggctgata ttctccgcag ccagactttt tccgccagac acgactttgt
     7441 agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac
     7501 tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac
     7561 agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag
     7621 gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt
     7681 cagggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat
     7741 gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc
     7801 ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc
     7861 catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc
     7921 gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt
     7981 accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc
     8041 ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg
     8101 ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat
     8161 acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtcagtcta
     8221 ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg
     8281 tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg
     8341 taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata aaggagcttt
     8401 gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc
     8461 gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg
     8521 cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgattacca gacccgctct
     8581 aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt
     8641 aacggtatcc ctctggcact caaccgcact atctaccaga tagaaatgat gccggagaaa
     8701 gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct gaccgatgac
     8761 gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattcccgtg
     8821 aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg
     8881 ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac
     8941 gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc
     9001 aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga gcgttactat
     9061 gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt
     9121 gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg
     9181 gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggta
     9241 gtcaccgatc cgcgtacagg tggggtgctg gcactggttt ccacgcctag ttatgaccca
     9301 aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgctgaa cgacccgaat
     9361 acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ctgcgtctac agttaaaccc
     9421 tatgtggcgg tttcggcatt aagcgccggg gtgatcacgc gcaatacgac gctgtttgac
     9481 ccaggctggt ggcaactacc agggtcggaa aaacgttatc gtgactggaa aaaatggggc
     9541 cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag
     9601 gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt
     9661 cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg
     9721 aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggc
     9781 cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat
     9841 gacggtatcg tgaaggttcc gcatttgctg atgagcaccg ccgaagacgg caaacaggtg
     9901 ccatgggtac aaccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg
     9961 gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca taaatacttt
    10021 gccagcgcac cgtacaaaat tgcggcgaaa tctggtaccg ctcaggtctt cggtctgaaa
    10081 gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg
    10141 accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggc
    10201 ggcgcgggtc cggcggttgg tacgctgatg cgccagatcc tcgaccacat tatgctgggt
    10261 gataacaata ccgatctgcc tgcggaaaat cctgcggttg ccgcagcgga ggaccattaa
    10321 tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa
    10381 tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg agcgccagcg
    10441 gtcaggatat tggcatgatg gagcgtaaaa taggccaaat cgcgatgggt ctggtcatca
    10501 tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata
    10561 tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct aaaggcgctc
    10621 aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg
    10681 taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg ttgaagaaca
    10741 ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc
    10801 tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta
    10861 gctggcgtct gattggcgtc gcagtagtgc tggtggcggc gttcattcct attctgtggt
    10921 tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctggac ccggaatcag
    10981 acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat
    11041 tacgcggcaa aggctggctg catggcactc agtcacaact tgaatttcta cccgaacgcc
    11101 ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc attctgattc
    11161 tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa
    11221 ccacctttgg tcgcgtcatg gctggcggct taatgctgat attattcgtt tatgtcttcg
    11281 taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca
    11341 gttatggagg atcagcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc
    11401 acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg
    11461 ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa
    11521 cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg
    11581 gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt
    11641 aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc
    11701 tatgatgccg aaccaggcag taacctgacg gcctctggcg aagctttcga tccgacacag
    11761 ttgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct
    11821 aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cctacggcaa cgaccgcgta
    11881 atttcgcttt ctcgcgcagc agctgaccgt cttaacacgt caaacaacac caaagttcgt
    11941 atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc
    12001 acagtcgcca aacagactta cgccctgcct gcacctcccg atttaagcgg tggcgcgggg
    12061 gcaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaactc gacgctaaaa
    12121 agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc tccaacgacc
    12181 ttagcgcctg gtgtactgga aagcagcgaa ccgacacctg caccacagcc cgttgttaca
    12241 gctccgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa
    12301 agcgccagcg gcaactttat ggtgcaagtc ggggccgtta gcgatcaggc tcgtgcgcaa
    12361 cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc
    12421 gcggtctggc gtatccagct tggcccattc gccagtaaag ccgaagccag taccttgcag
    12481 caacgtttgc aaaccgaagc ccaattacag tcatttatta ccaccgcgca gtagcgtaaa
    12541 gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag
    12601 atgcctgccg gtagtgcatt tgctatagta gggcactttt tttaattcca tcacggatgt
    12661 cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc tggcgctcac
    12721 cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga atatcaaaac
    12781 tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac atcctgattg actataactc
    12841 cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc gatcctgcta gcctgaccaa
    12901 aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta aagaaactga
    12961 tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta aaggttcttc
    13021 gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc gcggtattaa
    13081 cctgcaatcg ggtaacgatg cttgcgtcgc catggccgat tttgccgctg gtagccagga
    13141 cgcttttgtt ggcttgatga acagctacgt taacgccctg ggcctgaaaa acacccactt
    13201 ccagacggta catggtctgg atgctgatgg tcagtacagc tccgcgcgag atatggcgct
    13261 gatcggccag gcattgatcc gtgacgtacc gaatgaatac tcgatctata aagaaaaaga
    13321 atttacgttt aacggtattc gccagctgaa ccgtaacggc ctgttatggg ataacagcct
    13381 gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaacc ttgttgcttc
    13441 tgcgactgaa ggccagatgc gcttgatttc tgcggtgatg ggcggacgta cttttaaagg
    13501 ccgtgaagcc gaaagtaaaa aactgctaac ctggggcttc cgtttcttcg aaaccgttaa
    13561 cccactgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg attctgatcg
    13621 cgcttcgtta ggggttgata aagacgtgta cctgaccatt ccacgtggtc gcatgaaaga
    13681 tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc aaaagaatca
    13741 ggtcgtcgga actatcaact tccagcttga tggcaaaacg atcgagcaac gcccgctggt
    13801 tgtgttgcaa gaaatcccgg aaggtaactt cttcggcaaa atcattgatt acattaaatt
    13861 aatgttccat cactggtttg gttaaaaatt gaacacttga aagtgtaatt tccgtcccca
    13921 tatactaagc atcagtaaaa aaactcccgc cttctggcgg gagttgctat ttaattacgt
    13981 tacgccggag ctgacatgaa aaccaaactt aacgaactgc ttgaattccc tactcctttt
    14041 acttacaaag ttatggggca ggcgttacct gagctggttg atcaggtggt tgaagtggta
    14101 cagcgccatg cgccaggtga ctacacccca acggtaaaac caagcagcaa aggcaactac
    14161 cactcggtat ctatcactat caacgccact catatcgagc aggttgaaac actgtatgaa
    14221 gaactgggca aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc gttacccggc
    14281 tcctggtcgg gtaactcccc tctcctcgct gtgatatact tttcccccac ttttactcat
    14341 tctccacgga gatgccgttt tgtatcagga taaaattctt gtccgccagc tcggtcttca
    14401 gccttacgag ccaatctccc aggctatgca tgaattcacc gatacccgcg atgatagtac
    14461 ccttgatgaa atctggctgg tcgagcacta tccggtattc acccaaggtc aggcaggaaa
    14521 agcggagcac attttaatgc cgggtgatat tccggtgatc cagagcgatc gcggtgggca
    14581 ggtgacttat cacgggccgg gacaacaggt gatgtatgtg ttgcttaacc tgaaacgccg
    14641 taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga ataccctggc
    14701 tgaactgggt atagaagcgc atcctcgggc tgacgcgcct ggtgtctatg tcggggaaaa
    14761 gaaaatttgc tcactgggtt tacgaattcg acgcggttgt tcattccacg gtctggcatt
    14821 aaacgtcaat atggatcttt caccattttt acgtattaat ccttgtgggt atgccggaat
    14881 ggaaatggct aaaatatcac aatggaaacc cgaagcgacg actaataata ttgctccacg
    14941 tttactggaa aatattttag cgctactaaa caatccggac ttcgaatata ttaccgctta
    15001 attccataca tcaatggccc aatttacact gggtcattac tcataatacc tgctaatgct
    15061 ttacatctcc ttcgcgtttg tatattttct ttatcacttg cagaaatgcg gtgccacgcc
    15121 ttgctccgca actcacccca ttgattaacc tattgaaata gttgattatg gtgagcaaaa
    15181 gcgt
//
LOCUS       DAZFRJ010000110        14998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000110 DAZFRJ010000000
VERSION     DAZFRJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.111"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..2356)
                     /gene="maeB"
                     /locus_tag="ACHKUN_003044"
     CDS             complement(77..2356)
                     /gene="maeB"
                     /locus_tag="ACHKUN_003044"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO6661261.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2645..3595
                     /gene="tal"
                     /locus_tag="ACHKUN_003045"
     CDS             2645..3595
                     /gene="tal"
                     /locus_tag="ACHKUN_003045"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6661262.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3615..5618
                     /gene="tkt"
                     /locus_tag="ACHKUN_003046"
     CDS             3615..5618
                     /gene="tkt"
                     /locus_tag="ACHKUN_003046"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6661263.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5714..6757)
                     /gene="ypfG"
                     /locus_tag="ACHKUN_003047"
     CDS             complement(5714..6757)
                     /gene="ypfG"
                     /locus_tag="ACHKUN_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO6661264.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6883..7458)
                     /gene="nudK"
                     /locus_tag="ACHKUN_003048"
     CDS             complement(6883..7458)
                     /gene="nudK"
                     /locus_tag="ACHKUN_003048"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO6661265.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7526..9505)
                     /gene="aegA"
                     /locus_tag="ACHKUN_003049"
     CDS             complement(7526..9505)
                     /gene="aegA"
                     /locus_tag="ACHKUN_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO6661266.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9711..11411
                     /gene="narQ"
                     /locus_tag="ACHKUN_003050"
     CDS             9711..11411
                     /gene="narQ"
                     /locus_tag="ACHKUN_003050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO6661267.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11575..14688
                     /gene="acrD"
                     /locus_tag="ACHKUN_003051"
     CDS             11575..14688
                     /gene="acrD"
                     /locus_tag="ACHKUN_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO6661268.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14787..14846)
                     /gene="ypfM"
                     /locus_tag="ACHKUN_003052"
     CDS             complement(14787..14846)
                     /gene="ypfM"
                     /locus_tag="ACHKUN_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO6661269.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3524 a         3982 c         3912 g         3580 t
ORIGIN      
        1 aataagcttt actaatatat tgcagccaaa ccgcttaccc tgaatattca gggtaagcgt
       61 gagagttaaa aaagaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca
      121 tgttgacgat acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt ttcgcaacgc
      181 ccatcagcac cggaccgaca gtcacacctt ccgagctgga aacgcgcagt aagttgtaac
      241 taatgcgggc agcttccatg ttcggcatca ccagaatatt ggcagaacct ttcaaagggc
      301 tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac
      361 catcaatcat cagatctggc gcacgatccc tgaccagttc cagcgcctga cgcattttgc
      421 tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga caacagagca acgcgcggct
      481 caataccaaa acgacggaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg
      541 cgtctggttc gtcattgacg taggtatcgg caataaaggt gttaccactc ggcagcagca
      601 gcgcgttcat tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca
      661 cgctaaaatg ttcatgataa tcgcccaccg taccgcagat cattgcatcg gcttccccac
      721 gctgaaccat gatcgcgccg atcactgtcg ggttactgat cagcgcccgc tgcgcctgtt
      781 cctgagtgac accgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc
      841 gcggatcgga ttcgttattg acgatctcaa aatcaacgcc cgctttgatc tgcaagccca
      901 gtttctgaat gcgcatttcg atcacgttcg gacgaccgat aaggatcggt ttcgccagtc
      961 ccagcgttac cagttcctga gtggcatgca gaacgcgcgc ctcttcccct tctggcagaa
     1021 caacgcgctt cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttggttt
     1081 tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg
     1141 ccacgcccga ctccatcgcg gctttagcga ccgcaggagc gatcttaacg atcaagcgcg
     1201 gatcaaacgg ttttggaatg atgtattccg gaccaaagct cagatcctga tcgccatacg
     1261 ctgaagccac cacttcgctc tgttcggcgt gggccagttc cgcaatcgcg cgtaccgccg
     1321 ccagtttcat ctcttcattg atagccgttg cgccaacatc cagcgcgcca cggaagataa
     1381 acgggaagca aaggacgttg ttgacctggt ttggatagtc agaacgaccg gtgcaaatga
     1441 tggcatccgg acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca
     1501 gcgccaggat cattggcgca cgagccattt tcttcaccat ttcctgggtc agcactttcg
     1561 ggccggaaca tccaaggaaa atatccgcgc cttcaatcac atcatcgagg gtacgtttgc
     1621 cgtcatccac caccgcatat gcggctttgg tttccgccat gtttggctca cgcccctgat
     1681 agataacgcc ttttgaatcg caaaccacga tgttatgttt ttgcagaccc agcgctacca
     1741 gcaggttcat acaggcgatt gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg
     1801 agatgttttt ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg atgattgccg
     1861 tgccgtgctg atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa
     1921 tatagaaaca ttctggcgct ttaatgtctt cgaggttgat tccgccgaag gtcggttcga
     1981 gcgcagcgac aacttcaata aatttgtccg ggtcgagttc atcaacttca atgtcaaata
     2041 catcaatccc ggcgaatttc ttaaacagaa cgcccttgcc ttccatcacc ggtttgccag
     2101 ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc gttagagatc accgccacca
     2161 ggttaccgcg cgcggtatat ttgtaggctt ttaacgggtc tttttcgatt tcaagacaag
     2221 gtgcggcaac gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg
     2281 ttggagaaac ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt
     2341 ttaactggtc atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg
     2401 caacagcccc tgcccacaac gtggcgcaca ttattaccct gccggagtct acagactttg
     2461 agcaagtcca aactctcacc attaatataa tgttttggta ataatcctat aacactgatg
     2521 ttacctgctt aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttagcg
     2581 aaatttctca gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt
     2641 tcctatgaac gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga
     2701 tattgagtcc attcgccatt atcatcccca ggatgccacc accaatcctt cgctgttact
     2761 caaagctgcc ggattatcac aatatgagca tttaatagac gatgctatcg cctggggtaa
     2821 aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac tggcagtcaa
     2881 tttcggtgct gaaattctca aaatcgtacc cggtcgcgtg tcaacagaag ttgatgcacg
     2941 cctctctttt gataaagaaa agagtattga aaaggcgcgc catctggtgg acttgtatca
     3001 gcaacaaggc gttgagaaat cacgcatttt gatcaaactg gcttcgacct gggaaggaat
     3061 tcgcgcggcc gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc
     3121 ttttgcccag gcgcgggcct gtgcagaagc gggcgttttt ctgatttcgc cgtttgtcgg
     3181 gcgtatttat gactggtatc aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga
     3241 tccgggcgtt aaatcggtgc gcaatatcta cgactactat aagcaacacc actatgaaac
     3301 cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga
     3361 tcgactgact atcgcaccga atttgctgaa ggagctgcag gaaaaagttt cgccagtggt
     3421 acgtaaatta atcccgcctt ctcagacgtt cccacgccca gcacccatga gcgaagcgga
     3481 gttccgttgg gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg
     3541 tctgttcgct gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag
     3601 ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg
     3661 atgcggtaca aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg
     3721 ccgaagtgct gtggaacgat tttcttaaac ataatcctac cgacccaacc tggtatgatc
     3781 gcgaccgctt tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctacatc
     3841 tgaccggtta cgacctgccg ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa
     3901 ccccaggcca cccggagatt ggctatacgc caggcgttga aaccaccacc ggcccgcttg
     3961 gacaaggttt ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt
     4021 ttaaccagcc agaccatgag atcgtcgatc acttcaccta tgtgtttatg ggcgacggct
     4081 gcctgatgga aggtatttcc cacgaagtct gttcgctggc aggcacgctg ggactgggca
     4141 agctgattgg tttttacgat cacaacggta tttccatcga cggtgaaaca gaaggctggt
     4201 ttaccgacga tacggcaaaa cgttttgaag cctatcactg gcatgtgatc catgaaatcg
     4261 acggtcacga tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc atgaaagata
     4321 agccgtcgct gattatctgc cgtacggtga ttggctttgg ttcgccgaat aaagcaggta
     4381 aggaagaggc gcacggcgca ccactggggg aagaagaagt ggcgctggca cggcaaaaac
     4441 tgggctggca ccatccgcca tttgagatcc ctaaaaagat ttatcacgcc tgggatgccc
     4501 gcgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc tataaaaagg
     4561 ctcatccgca actggcagaa gagtttaccc gtcggatgag cggtggttta ccgaaggact
     4621 gggagaaaac gactcagaaa tatatcaatg agttgcaggc gaatccggcg aaaatcgcca
     4681 cccgtaaggc ttcgcaaaat acgcttaacg cttacggacc gatgctaccg gagctgctcg
     4741 gaggttcagc ggatctggct cccagcaacc tcaccatctg gaaaggctct gtgtcgctga
     4801 aggaagatcc ggcgggcaac tacattcact acggggtgcg tgaatttggc atgaccgcta
     4861 tcgccaacgg cattgcgcac cacggcggct ttgtgccgta taccgcgacg ttcctgatgt
     4921 ttgttgaata cgcccgtaat gccgcgcgga tggcggcact gatgaaagca cggcagatta
     4981 tggtttatac ccacgattct attggcttag gggaagatgg tccgacgcac caggccgttg
     5041 agcaactggc cagtctgcgc ttaacgccga atttcagcac ctggcgaccg tgcgatcagg
     5101 tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca caacgggccg acagcactga
     5161 tcctctcaag gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg
     5221 ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc agatattatt ctgattgcca
     5281 ccggttcaga gatggaaatt accctgcaag cggcagagaa attagcagga gaaggtcgca
     5341 atgtacgcgt agtttccctg ccctcgaccg atattttcga cgcccaggat gaggaatatc
     5401 gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg
     5461 attactggta caagtatgtt ggtctgaaag gggtaattgt cgggatgacg ggttacgggg
     5521 aatctgctcc ggcggataag ctgttcccgt tctttggctt taccgccgag aatattgtgg
     5581 caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg gatgctgatt
     5641 gccggatgcg acgctgacgc gtcttatccg gcctacagcg tcttatccgg cctacatgtc
     5701 cccgcaattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc ccatgccagt
     5761 tatcgcaggt gggttctgcg gcataacgca ccaggcgaaa acgctgaccg tcaaagcgcc
     5821 agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc accagttcac
     5881 gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc tgaccgttgt
     5941 tgaatggcaa acgcaaccga accgggcgcg aggctagtgg ctttttacgc gacacaatcc
     6001 atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc agcgctttat
     6061 catcagtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag cagcgcagac
     6121 cattcatccg ccagtttcca taatccagca aatcgttgcg ttcttcgagt gagagtggcg
     6181 tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt acgctgagcg
     6241 gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt tgctgagcat
     6301 caataaacaa caacgccgct tttaagccac tcagagaaat ggtctgattg ccgtcgcgta
     6361 aggtgatcgc cttcccttcc tgaatcacct gcaaaaacgc ggtgatggtt gccgtatcat
     6421 cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaacgcc aacggctcac
     6481 catctaacag cagccgtggc gctatctctc cttcagacgc atccggcgac ttcaacccgc
     6541 cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc agggtcatca
     6601 ccagtccatt atgatcgccc gtattgcgcg ccacgcagaa gttttggtta ttacaggtga
     6661 cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac gtcggcaaca
     6721 aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc ccagaagttc
     6781 aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat cagatttgtc
     6841 ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca ttaaatgtga
     6901 cgtttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc cggttttgat
     6961 catctccagc gcctggctga acggcagctc gagcacttca atatcttcat cttcaacacc
     7021 gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt ggattagctc
     7081 agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc ccacttcata
     7141 gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt tatccagcag
     7201 cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg tagcgacacg
     7261 gaactgacga atcagaacca cgctcttttt cttcgcgttg tacaggagga tcgtcgcacc
     7321 attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt tgcgcgtgag
     7381 atcgtaggta atgttgtgca gggtgaaata gttatcggag agaattttgt ctttaatgag
     7441 ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattatc ttacgctgtg
     7501 aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc cccagccagt
     7561 caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc agatccgcac
     7621 cacgcacggc gtcgccaccg gcgaagattt tcggattggt ggtctggtaa cggtactggc
     7681 tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc gactccagcc
     7741 acggcatccc gtgtggattg aagccaaacg ccataatcac cgcatcggct ggcatgacaa
     7801 attcactgcc ctcaatcggt acaggtcgcc gacgcccctg ggcatccggc tcgccaagac
     7861 gcgtgcgcag gaaacgaatc ccgcagacgt gaccttgttc attcagctca agcgccaccg
     7921 gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcctcacc tctttcttcg
     7981 agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc gcgccgtggc
     8041 gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa
     8101 gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc acctgtttag
     8161 tgttggcaat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac
     8221 ccgcgttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa
     8281 gcgaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga atccccatcg
     8341 cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa gaaggaatgc
     8401 cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc gccacgccat
     8461 tgcgggtcag aacatccgca caggccagcc ctgccggacc tgccccgata atcgccaccc
     8521 gcttgtccac tttggtgaca tgacttaagt caggacgcca acctttcgcc aacgcctgat
     8581 ctgaaatata gcgttcaata ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac
     8641 aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt
     8701 tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc agttcaatcc
     8761 actgcggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc tcgccgcact
     8821 taaggcagcg cgaggcttcc cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat
     8881 caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg
     8941 cctgcatttg ttcgacttta ctcattaccg gcatttcttg cgccgcggta ctggcatgcc
     9001 acggttgatg ttcctgacgc gcggtgcgca agcggcgggg tttcgccatg ccggagagtg
     9061 cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcaacacag gcaggaccgt
     9121 tttcgcgccc cgcacaaagg tcgcatttat gcgccgtggc ttttactttt cctgccgcga
     9181 cgggtgtcag gacgatttgc atcgtaccaa aaggacaggc caccacgcag gatttacagc
     9241 caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt gcgccattag
     9301 ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca ctacgttgct
     9361 gttgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg acatgttgct
     9421 catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga cattgctgac
     9481 tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa aaccttattc
     9541 tttatatgag tgttgttatt acccgactta caggggaatc ggcaatgttc atgtgcccag
     9601 aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca ctgactaaat
     9661 tgtgtttcag ctaactggaa cattaatgat tttttgtgga gaagacgcgt gtgattgtta
     9721 aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg ctgtcgattc
     9781 tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct gaggctatca
     9841 atattgccgg atcgctacgt atgcagagtt accgcctggg ctacgacttg caaagtggca
     9901 gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca ccggtattaa
     9961 ccaatctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg catctgaatg
    10021 ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg tatcaggcca
    10081 atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag cactacgctg
    10141 aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt attttcacgc
    10201 tggtcttttt tactttgcgc cgtatacgcc atcaggtggt tgccccgctg aatcagctgg
    10261 ttaccgccag tcaacgtatt gaacacgggc agttcgactc gccgccgctg gataccagtc
    10321 tgccgaatga gcttggtctg cttgcaaaaa cctttaacca gatgtcgagc gagctgcata
    10381 aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca
    10441 agcgtcgtct ggaggtgttg tatcaatgtt cccaggcgct gaacaccagc cagattgatg
    10501 tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcggct gaatatctgg
    10561 agttaaatgt cggtgaaaac tggcggatta gcgaagggca accaaacccg gaattgccga
    10621 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata
    10681 gtcacgtttc atcatcagaa ccgctgctta acagtgtttc atcgatgctg ggacgcggtt
    10741 tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa gaacgtgcaa
    10801 ccatcgcccg cgaattgcac gactcgctgg ctcaggtact ttcttactta cgtatccagt
    10861 tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt atcatggccg
    10921 atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg actactttcc
    10981 gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg gatacgttac
    11041 aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg gcgctggatg
    11101 cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc
    11161 acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg
    11221 tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa ggtcattatg
    11281 gtctgaatat catgcgcgaa cgcgcggaac ggctgggtgg gacgctgact ttttcacaac
    11341 cttccggcgg cggcacgtta gtgagtatta gctttcgctc tgcggagggt gaggaaagtc
    11401 agttgatgta atgcctccta ctgaccaaag aatacttgca cttaaggttc agtataaaag
    11461 ggcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta
    11521 cagtgaagca agtcaagcct acaacgatac gcagaaacac gaggtcctct tttaatggcg
    11581 aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt gtgtctgaca
    11641 gggaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat
    11701 gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc
    11761 caggttatcg agcaaaatat gaccggcctc gataatctca tgtatatgtc atctcagagc
    11821 agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa
    11881 gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg
    11941 caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca atattctgac cattgctttc
    12001 gtctctaccg atggttcgat ggataaacag gatattgccg attatgttgc cagtaatatt
    12061 caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat
    12121 tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat
    12181 gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtaca
    12241 ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact gctgcaaaca
    12301 ccagaacagt tccgcgatat caccttgcgg gtgaatcaag acggctcaga ggtaaggctg
    12361 ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag ccgcttcaat
    12421 ggtaagccag cctccgggct gggggtaaaa ctggcctccg gcgcaaacga aatggcgaca
    12481 gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt acttcccgca cggactggaa
    12541 tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa
    12601 acgctgctgg aagctatcgc cctggttttc ctcgttatgt atctgttcct gcaaaacttc
    12661 cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta
    12721 ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt actggcgatc
    12781 ggtctgctgg tggatgacgc aatcgtggtg gtggaaaacg tcgaacgtat tatgagtgag
    12841 gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg
    12901 gtcgggattg cgatggttct ttcggcggta tttgtaccaa tggccttctt cggcggcacc
    12961 accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta
    13021 ctggtagcga tgatcctcac tccggctctg tgtgccacac tacttaagcc actgaaaaaa
    13081 ggtgagcatc acgggcaaaa aggctttttt gcctggttta accagatgtt taaccgcaac
    13141 gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg
    13201 atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc
    13261 ttaccgctgg aagaccgtgg catgtttact acctcggtac aattgcccag cggttcaacc
    13321 caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcac ccatgaaaaa
    13381 gacaacatca tgtcggtgtt tgccactgtt ggttctggcc ccgggggtaa cgggcaaaac
    13441 gtggcgcgaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc
    13501 tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga agctcgcgtt
    13561 atcgccagca gcccgccagc aattagcggt cttggtagtt ctgcaggttt tgatatggag
    13621 ttgcaggacc acgctggagc gggtcacgat gcgctgatgg cagcacgtaa tcagttgctg
    13681 gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgacgacagt
    13741 ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgctatcgac
    13801 gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat
    13861 cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat
    13921 gacattaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc
    13981 gcgacctcac gctgggaaac aggctcgccg cgtctggaac gctataacgg ttattctgcg
    14041 gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg
    14101 gaatcgttag tgaagcagtt gccaaacggc tttggtctgg agtggacggc gatgtcgtat
    14161 caggagcgac tttccggcgc gcaggctccg gcgctgtacg ccatttcctt gctggtggta
    14221 ttcctgtgtc tggcggcgtt gtatgaaagc tggtcggtgc cgttctcggt aatgctggtc
    14281 gtgccgctgg gggttatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac
    14341 gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg
    14401 atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga agcgacgctc
    14461 cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt tatcttcggc
    14521 gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact
    14581 ggcgtaatgg gcgggatgat ttcagctact attctggcta tttacttcgt gccgctgttc
    14641 tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag
    14701 gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc
    14761 ttgctgttta tactttgtct cgggaattac ttacgaagca taacttcgat aaagtctttc
    14821 cagttcccca gttcacgttc aatcataaca acctctctta taattatggg tattctacgg
    14881 aaacaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac ctccggggga
    14941 caaaccttat gtaaatacta tggtcctgcg gtgataattt gtatgtgata cacagcaa
//
LOCUS       DAZFRJ010000111        14976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000111 DAZFRJ010000000
VERSION     DAZFRJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.112"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..146)
                     /locus_tag="ACHKUN_003053"
     tRNA            complement(71..146)
                     /locus_tag="ACHKUN_003053"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Phe,seq:gaa)
     gene            complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKUN_003054"
     CDS             complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHKUN_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO6661270.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKUN_003055"
     CDS             1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHKUN_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO6661271.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1357..3492
                     /gene="speC"
                     /locus_tag="ACHKUN_003056"
     CDS             1357..3492
                     /gene="speC"
                     /locus_tag="ACHKUN_003056"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO6661272.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKUN_003057"
     CDS             complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHKUN_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO6661273.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKUN_003058"
     CDS             complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHKUN_003058"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO6661274.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKUN_003059"
     CDS             complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHKUN_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO6661275.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKUN_003060"
     CDS             complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHKUN_003060"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO6661276.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKUN_003061"
     CDS             7660..8379
                     /gene="trmB"
                     /locus_tag="ACHKUN_003061"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO6661277.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKUN_003062"
     CDS             8379..8705
                     /gene="yggL"
                     /locus_tag="ACHKUN_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO6661278.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKUN_003063"
     CDS             8889..9608
                     /gene="yggN"
                     /locus_tag="ACHKUN_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO6661279.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKUN_003064"
     CDS             9784..10830
                     /gene="ansB"
                     /locus_tag="ACHKUN_003064"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO6661280.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKUN_003065"
     CDS             10947..11954
                     /gene="yggM"
                     /locus_tag="ACHKUN_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO6661281.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQAFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12010..13311)
                     /locus_tag="ACHKUN_003066"
     CDS             complement(12010..13311)
                     /locus_tag="ACHKUN_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6661282.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13311..13814)
                     /locus_tag="ACHKUN_003067"
     CDS             complement(13311..13814)
                     /locus_tag="ACHKUN_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6661283.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13859..14845)
                     /locus_tag="ACHKUN_003068"
     CDS             complement(13859..14845)
                     /locus_tag="ACHKUN_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO6661284.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3973 a         3588 c         3784 g         3631 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
      121 tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc
      181 gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat
      241 tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat tgccagaagg
      301 agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt
      361 gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc
      421 ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta
      481 attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga
      541 aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag
      601 atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaagat
      661 tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc gactgctgta
      721 ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag cgtagctaaa
      781 accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc cagcgatgcc
      841 agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg gctgagcagt
      901 gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc gatgaccaca
      961 agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc cttgttgatg
     1021 gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg
     1081 aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc
     1141 ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc ggtgaagtta
     1201 acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg
     1261 gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt tccaccttgt
     1321 ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa tattgccgcc
     1381 agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt gggagatact
     1441 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     1501 gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     1561 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg gctggagctg
     1621 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     1681 cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca tggtgcgttt
     1741 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     1801 gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     1861 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtgctg
     1921 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg
     1981 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     2041 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     2101 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     2161 gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggaact
     2221 gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta cattctgttt
     2281 gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag ctcgccgctg
     2341 ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt gcacaaacag
     2401 caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
     2461 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
     2521 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
     2581 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
     2641 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
     2701 gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
     2761 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
     2821 ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt tggcgttccg
     2881 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
     2941 aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc acaactggtg
     3001 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
     3061 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
     3121 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
     3181 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
     3241 cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg
     3301 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
     3361 gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg attttcgccg
     3421 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
     3481 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
     3541 attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg
     3601 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
     3661 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
     3721 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
     3781 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
     3841 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
     3901 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
     3961 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
     4021 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
     4081 tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt tccgcgccca
     4141 gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
     4201 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
     4261 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
     4321 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
     4381 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
     4441 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
     4501 atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac agcgtgatag
     4561 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
     4621 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
     4681 tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc gtcagccaac
     4741 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
     4801 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
     4861 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
     4921 acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc ctgagcatcg
     4981 tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
     5041 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga taaacatcgc
     5101 ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa aagactcgca
     5161 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc gatgttgggt
     5221 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
     5281 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
     5341 ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc gcatcggaac
     5401 ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga atcagcgact
     5461 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
     5521 tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc agcccgttgc
     5581 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc
     5641 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
     5701 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcagca
     5761 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atagtttcga
     5821 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
     5881 tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg ttctcaatat
     5941 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
     6001 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
     6061 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc
     6121 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
     6181 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
     6241 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
     6301 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc ggggtacagc
     6361 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
     6421 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
     6481 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
     6541 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
     6601 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
     6661 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
     6721 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
     6781 tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
     6841 ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag cggacagagc
     6901 gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat cgcctgatta
     6961 aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa tttattctcg
     7021 acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
     7081 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc acctgcggtg
     7141 gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa tttaccgccg
     7201 tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc gtaatagcca
     7261 agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
     7321 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
     7381 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagcgtt
     7441 ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
     7501 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
     7561 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
     7621 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
     7681 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
     7741 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
     7801 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
     7861 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccctgag
     7921 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
     7981 catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
     8041 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
     8101 aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact ggtaaaaagc
     8161 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
     8221 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
     8281 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
     8341 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
     8401 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     8461 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     8521 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     8581 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     8641 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     8701 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     8761 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     8821 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     8881 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     8941 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     9001 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     9061 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     9121 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     9181 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     9241 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     9301 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     9361 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     9421 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     9481 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
     9541 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     9601 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     9661 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     9721 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     9781 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     9841 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     9901 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     9961 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    10021 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
    10081 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
    10141 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    10201 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt ggccgcagct
    10261 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
    10321 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
    10381 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    10441 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
    10501 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    10561 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
    10621 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
    10681 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    10741 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
    10801 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
    10861 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    10921 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
    10981 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
    11041 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
    11101 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    11161 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
    11221 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    11281 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    11341 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    11401 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
    11461 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    11521 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
    11581 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    11641 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    11701 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacaggct tttctgcaat
    11761 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa gggcaatatc
    11821 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
    11881 acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg cggcgcaatg
    11941 gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag ttatccggca
    12001 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
    12061 agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg gtttgattaa
    12121 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
    12181 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
    12241 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
    12301 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
    12361 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
    12421 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
    12481 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa ccagcaccga
    12541 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
    12601 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    12661 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
    12721 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    12781 aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    12841 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    12901 atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    12961 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    13021 tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
    13081 gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata gggcaaagtt
    13141 atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc agggaaacat
    13201 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
    13261 gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca ttaactggcc
    13321 tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa ggcataaaac
    13381 atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg tatttgcatc
    13441 gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc accagtcaca
    13501 atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag aagtaattgc
    13561 ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg cgcgccgacg
    13621 gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt
    13681 aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc
    13741 agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg
    13801 cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc ccggaatatt
    13861 attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt
    13921 cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac
    13981 cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt
    14041 gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct ggggttaatt
    14101 tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag
    14161 aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg
    14221 cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg
    14281 gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga acacgaattt
    14341 tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac
    14401 ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt
    14461 taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca
    14521 aattatagat agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca
    14581 tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc atctggctgc
    14641 tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct
    14701 cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca
    14761 ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg
    14821 agcgggtaaa tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg
    14881 atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt ctgacaactt
    14941 gtctatgttt atcacaactt gaaataaagt cagaaa
//
LOCUS       DAZFRJ010000112        14972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000112 DAZFRJ010000000
VERSION     DAZFRJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.113"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            167..823
                     /gene="pphA"
                     /locus_tag="ACHKUN_003069"
     CDS             167..823
                     /gene="pphA"
                     /locus_tag="ACHKUN_003069"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6661285.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(824..1015)
                     /gene="yebW"
                     /locus_tag="ACHKUN_003070"
     CDS             complement(824..1015)
                     /gene="yebW"
                     /locus_tag="ACHKUN_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO6661286.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1120..1356)
                     /gene="yebV"
                     /locus_tag="ACHKUN_003071"
     CDS             complement(1120..1356)
                     /gene="yebV"
                     /locus_tag="ACHKUN_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO6661287.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1474..2913)
                     /gene="rsmF"
                     /locus_tag="ACHKUN_003072"
     CDS             complement(1474..2913)
                     /gene="rsmF"
                     /locus_tag="ACHKUN_003072"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO6661288.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(2993..5626)
                     /gene="yebT"
                     /locus_tag="ACHKUN_003073"
     CDS             complement(2993..5626)
                     /gene="yebT"
                     /locus_tag="ACHKUN_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO6661289.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5595..6878)
                     /gene="yebS"
                     /locus_tag="ACHKUN_003074"
     CDS             complement(5595..6878)
                     /gene="yebS"
                     /locus_tag="ACHKUN_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO6661290.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7008..7505
                     /gene="msrC"
                     /locus_tag="ACHKUN_003075"
     CDS             7008..7505
                     /gene="msrC"
                     /locus_tag="ACHKUN_003075"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO6661291.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7602..8300
                     /gene="proQ"
                     /locus_tag="ACHKUN_003076"
     CDS             7602..8300
                     /gene="proQ"
                     /locus_tag="ACHKUN_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO6661292.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8320..10368
                     /gene="prc"
                     /locus_tag="ACHKUN_003077"
     CDS             8320..10368
                     /gene="prc"
                     /locus_tag="ACHKUN_003077"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO6661293.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            10560..11441
                     /gene="htpX"
                     /locus_tag="ACHKUN_003078"
     CDS             10560..11441
                     /gene="htpX"
                     /locus_tag="ACHKUN_003078"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO6661294.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11487..12860)
                     /gene="yebQ"
                     /locus_tag="ACHKUN_003079"
     CDS             complement(11487..12860)
                     /gene="yebQ"
                     /locus_tag="ACHKUN_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6661295.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLKPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            13037..13828
                     /gene="kdgR"
                     /locus_tag="ACHKUN_003080"
     CDS             13037..13828
                     /gene="kdgR"
                     /locus_tag="ACHKUN_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO6661296.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13971..14210)
                     /gene="yobH"
                     /locus_tag="ACHKUN_003081"
     CDS             complement(13971..14210)
                     /gene="yobH"
                     /locus_tag="ACHKUN_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO6661297.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14369..14512
                     /gene="mgrB"
                     /locus_tag="ACHKUN_003082"
     CDS             14369..14512
                     /gene="mgrB"
                     /locus_tag="ACHKUN_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO6661298.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14587..14874
                     /gene="yebO"
                     /locus_tag="ACHKUN_003083"
     CDS             14587..14874
                     /gene="yebO"
                     /locus_tag="ACHKUN_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO6661299.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         3920 a         3653 c         3844 g         3555 t
ORIGIN      
        1 acgaaaacag gaatcgtgtt cggtctcttt ttatctgtta aaagccagaa gcatttcctt
       61 cgctgacttt atagtcaacc ataacacaca ctctactgtc tgagtccagc gttttttaac
      121 attcttgtta agattatgtg atctttagcg cgggaggaaa atattgatga aacagcctgc
      181 gcccgtttat cagagaattg cgggtcatca atggcgacat atctggcttt ctggcgatat
      241 acacggttgt cttgagcagt tgcgccgcaa attatggcat tgtcgttttg atccgtggcg
      301 agatttactt atctcagtag gagacgttat cgatcgtggg ccgcaaagtt tacgttgtct
      361 gcagttactg gaacaacatt gggtttgtgc ggtaagaggc aatcatgaac agatggcgat
      421 ggatgcgctg gcatcccagc agatgtcttt gtggttgatg aatggcggcg actggtttat
      481 tgcgctggca gataatcaac agaaacaagc gaaaacggcg ctggaaaaat gtcagcattt
      541 gccctttatt cttgaagtac acagtcgtac cggcaaacat gttattgctc atgccgatta
      601 tccagatgat gtttatgaat ggcaaaagga cgttgatttg catcaggtct tgtggagccg
      661 ctcgcgatta ggtgaacgcc aaaaagggca gggaattaca ggtgctgatc atttctggtt
      721 tggtcataca ccgttgcgac atcgcgtgga tattggcaac ctgcattata ttgataccgg
      781 tgctgtcttt gggggcgaac tgactcttgt gcaattgcaa taattaaaaa tcaccatact
      841 cctgtgctgg tcgccagaaa ccatctataa aatcctcaat cggaaaacaa ccgccatggc
      901 ggatccgttg atcgctcata gaataaagac actgctgttc cgtgttgtag acatccacaa
      961 caatatcttc acaaccgcca tccaggtagc aaacaaaaag taccagcgcg aacatttcat
     1021 ccccgaagtg tggtgccgta ccgttaagtt taggagagat tttacaacgg gggaataacc
     1081 aggacaaata acccgccatg ccgacgggtt ctttttggat caggcaagac gcataatcca
     1141 ggcatcagat tgttgatcgt aacgggtacg gtaaaggaca gagtgttcgc catttagcag
     1201 ggcagggata ctggtttcag catcccaggc atcgagttgc atacataaat cggggtcaga
     1261 tttacaagga atggttaacg ttcgatcacc cggcgattca ccgtgtaatt cgccgtcgtc
     1321 gatttcaaaa gcgcctatgc gcacactggt tttcatcttc gtgctctcca tttagctgcc
     1381 gattgtggta cgaccagtcc gatcgcacca tttttcagcc tagttgaggc agccgaatgt
     1441 gccagtcaaa aagtgcgctt tttttgtgag ctgtcaggcg ttaccggtaa aaagtttccc
     1501 atcgcgcacc agttcacgcg gatagctgtt tttcaatcgc gaaccaatcc gtttggctaa
     1561 accaatcggt tgatgctgga aagtaaccaa tacatcatcc gccactggcg cggcttgcgg
     1621 gtaaacatcg cgcccgcgat accactcctc cgcttcctgc ggtgtcagct cgaatgcatt
     1681 catattatcg ggtgaggcaa gagcaataac cgcttcatgc tgccagcgat aacctttgtt
     1741 gtgcgtctcg gcaagtttaa tccccaaccg agaaaatcgg actttaccga tcagggcttc
     1801 aatgcccacc gggaacaacc acagttcttt gtcacgctgc cagaggcgca ggttttcatc
     1861 ccagtttaag ccaacacctg cagtcgcctg acgaatttgt ccagcttcgc gatctttcac
     1921 cgggctgaac ggaaaattac cgactttgta tttgggggcg ggtaaggcgg gaatcgcctg
     1981 agttttacgc agacgagcaa cgaagaagcc ttcgcagtcg taaatttgtg ggaaaacatg
     2041 caaaaagcct tcttcggtca gcgctttgtt tgcaccaggg aagagatcgc caagtggtaa
     2101 aaactctact gcgtcggggt aagtctcttt cagccacagg caaacggctt cgttttcttc
     2161 ctggtttaag gtacaggtcg agtaaaccag cgtaccacca ggacgtaatg catgaaaggc
     2221 gctgtcgata agctcccgtt gtgtagctgc gatttcctga ttgctttctg gtgaccagtt
     2281 ttttagcgca tcgggatctt tacgcaccac gccttcgcca gagcagggag cgtccagcaa
     2341 aatggcatcg aacatttctg gcactgccgc accaaacacg cggccatcaa aatgtgtgag
     2401 cgcaacatta ctgatgccac agcggctgat attggcatgt aacactttta cccgactggc
     2461 ggaaaactca ttggcaagga ttgccccttc gttattcatc cgcgcggcaa tttgcgtcgt
     2521 tttggagcct ggcgcagctg cgacatccat cacccgctgt ggtgcattat cgtcagcaaa
     2581 caaggcggca acgggcaaca ttgaactggc ttcctgaata taaaacaggc cgcttaaatg
     2641 ctcggcggta ctacccaatg gcaatgcatc ttcattgtcg cgttcaatcc agaaaccttc
     2701 ttcacaccac ggaattggcg taagcgtcca gccataagga gcggttaatt gcaggaaatc
     2761 agcaacggag attttcagcg tattaacgcg aatgctgcgg cgcaacgggc gctgacaggc
     2821 ggcaagaaaa tcatcaaatg agagcgtcga aggcatcgct tcgcgcattt gtgtcagaaa
     2881 ggcgtccggg aaataaacgg tgtgttgggc cacgagcatg taccactggc aaaaaaacag
     2941 gcgcgcagtt tagcataaac gctccggcgc aggcacgccg gagcagtggg cattatttgg
     3001 gaagcgcagt tccccattca cgccactctt tcggttcact ttcctgcaac aggaagtgtt
     3061 tgccttcctg ggctttcggt gccagtggcg tacccggagg cgtggcgaag gcgatgccgc
     3121 cacggataaa ctggttaaag gtgccggttt tcactacgcc gcccgtcaga ccaaagtcca
     3181 gactgtaacc cgatgccaac cagaagacgg aattgttacg caccaggtgt tgatagcgtt
     3241 tactgatgcg catcgcaatc atcacgcgat ctgacaatgt ccccagcgtc attcctgtaa
     3301 ccgtaccgac ttccagacca cggaacagca caggcgtacc aatacctaac gaaccggctt
     3361 ccggcgcttc aacaataatg cttaagccat ccaggtaacg cgaatcagta atggtggcct
     3421 cttgtaattc aaagtcgcgg cgaggattgc cccggcctgg ttcgacgttg atatacggct
     3481 ggaggatagt atctagatgc tcaacgcccg cggctgaaat ttgcggtgtg accaccgaga
     3541 agcgcgtacc gccgcgggcg aaggtctgga catactccgg atagagcacc gcctttgcct
     3601 gtacttcatt gcgcgcggta atcagatcca gcgtctggat ttgcccgata tcaataccga
     3661 gatagcgaat tggcatcccg accgccagtt ttccggcatc gaaagcgtga agcgtaatct
     3721 gcccaccaac cgcacgggcc gctgtttcgg aagcatacag aattcgtttg tcgcctttac
     3781 gctgactggc gctggcaccg ctgaggttat cgaagctaat ggctcccttt aatgctctgg
     3841 agagcgggga tgcctgtacg gtcaggccac taccattcag ctgaactttc gccccgcctt
     3901 ctgcccagaa cacgctattg ctggtcagaa ggttgcgata ctccggctta atatgcagat
     3961 cgatatcaaa cgcgttagct cgcggacgga cggtaataac ttcaccaact tcaaatttac
     4021 ggtagagcac taccgatcct gcctgcacat ccggcagcgt ctctgcactc aaactcacgg
     4081 ttgtggtggg taaatcgcta aggctgttct ccagcgcttt ttccagattg gcatacagtg
     4141 gatagctggc tttcatctcg cctttatcgc ccggcagaat acgtatcccg ccgttaatcc
     4201 attctgaggc gctggcaccg agaaactcaa cgccatccag ccccaccttc acgtcgacac
     4261 ggctgttgac gacaaatttg ctatcgcctt ttaccagttc tcgatgctga ggctcgatgg
     4321 cgacggtaaa ggtgacgcct ttgctggtga gtttacgatc gataacctgg cctacctgca
     4381 cgccgtgaag aatgagcggc tgaccagcat caataccgta actttccggc gcggtcaggg
     4441 tcagcgtcag aacatcaggt tcatgcagca gtgctttttc gcctggcaca acaacgaact
     4501 ctttgcgtgg ctcgccatcg ccgggtacca actcgaaggt tttgccagtc agcagggcgc
     4561 tgagattggc atcgctaagg gataatttcg gattacgtaa ttcgatgcgg gtattttcac
     4621 gaagcagggt aacgacgctg ggatcaactg tcatttcccc agtgacttta ccaccaggat
     4681 ttaaatccag tttagttagc tgtccgactt ccagcccctg atacattaac ggcgtcgagt
     4741 cggcggtcaa tccggcccca ctcggcagtt ccagttttat tattacgcca cgctggctgt
     4801 gggccagatc ttcatacaga ccaaaggtat cttccgcctc ggcaggtttc gactcttcag
     4861 gtgaatcgaa ggcaatcgca ccgttaacca gtgccgccag actttccagt ttcaccttcg
     4921 cgccactgac actgacgttg gcatcaacgc cggaaacgtt ccagaaacgg ctaccttttt
     4981 tcaccagatc ggtaaaacgc cgctcgatca gaacatcaat caccacgcct tgcttgttgg
     5041 gattgatggc atagtcgtag acttttccca ccgggatctt gcggaaatag accaatgaac
     5101 cgctgttcag cgaaccgaga tcgggagctt gcaggtggat catcagatcg ccgttatcca
     5161 gccgatactt cggttgggta tcgagtgcga caaagtgatc ctgctcttta cctttacccg
     5221 gcatcatgcc gatatagttc ccaccgacga gggcgtccag cccggagaca cctgccaacg
     5281 atgcttttgg cgtcaccagc cagaattgag tctcttcgcg cagcgcatct ttcatatcgg
     5341 acttgatgct gaccttgact tcaatcttac gaagatcgtc gctgaggctg atatcctgca
     5401 ctgttccgac ttcaacgccc tgataacgaa caggcgtacg gcccggaaca ataccatccg
     5461 ccgacataaa gtcgatggtg acggtattac cccggtcctg ataactgtcc caaatcagcc
     5521 aaccggcaat cattagcgcg atgaaaggca gcagccagaa aggtgagata cggcgtttat
     5581 ttttaatctg cgcttcagtc gtcgaagcgg gcgtttcctg actcatgtgc atcccaaagt
     5641 aagcggctgt ccagccattc cacagcaaga atagtcaaaa ttacggctgc gccaaaataa
     5701 aacgcagccg gtcccatagt aaaagcgagg atctgatcgc gattaatcag cgacatggtt
     5761 aaagatatga caaacaggtc gagcatcgac cagcgtccaa tccaggtcac catccgcagt
     5821 aacagaatgc gtgtgcgtaa accttgctgg catttaaaat gaatgctcaa cagtaaagtg
     5881 aacatgacga tcactttagt aaacggtacc agaatactgg cgataaacac gattcccgca
     5941 accgcaatgt tgctacttgc cagcgacata attccggaaa gaattgtatc ttcctgccgt
     6001 cctccgttca gataaatgat agaaataggc aataggttgg ctggtaacaa caaaacgatt
     6061 gacgctaaca gcgccgccca gcatttttgc agactatgac ggcgacgcag gcgtagcggg
     6121 atatggcaac gcgggcagcg accacgctga tcaggatagc cggtaaaatg gcacccaaga
     6181 cagacacgaa gtttctcgtc cctacgcgta gcggggcgct gcggataata tcgctcccac
     6241 agctcctcaa cattaagatg tgacaacgtc accgtcgtta aaatcaccaa cgccacaaaa
     6301 gagaacaagc cgacaccagc ctggatatgg gcataatcct gtacctttat agaagcaacg
     6361 ccaatgccga ccaggtagat atccagcatt acccactctt taagacgctc aagcatcagc
     6421 agtaccggac gtaaattcat tcccagtcgg ttaccaaacc ataaatatgc tatggaggac
     6481 accagaatga ggggggcacc aataacgcag aaaaagacca tcgcccccgt tatcgtatcg
     6541 ccctgtttgg tcatttgcca gatgccttgc atgacgttgg cgtcgatacg aatacctaac
     6601 agccagatat gcaacagcgg ttcgccccag gcaaacggca tcagcaacag catggtgaaa
     6661 gccattgccg ccaggcgcgt tagcgaccag tcgcgcccgt cgcgaatttt tgcctgacag
     6721 cgcggacaat aggcactctg atgagaattt atctcgggca ggctaaacag catgtcacat
     6781 tgcgggcaac gttggtaatc accacgcggc agttcctcgc cgattgccct cactgttatc
     6841 ttttttgtcg gcgtaatttg tggtgtgtta agagccatga cgtgccaaat gtaataagaa
     6901 ttaacattaa tattaaccca tgattggaat gatcttgagc attaacgcat ttaatgctta
     6961 ttttaatagg ctggcagttc caggtaagac aaccaagtga ttatataatg aacaaaacag
     7021 aattttacgc ggatttaaat cgcgacttta acgcgctgat ggcgggagaa accagttttc
     7081 tggcaacgct tgcgaacacc agtgcgttgt tatacgagcg tctcactgac gtaaactggg
     7141 caggttttta tttgcttgag gacgatacac tggtactagg accatttcag ggcaaaattg
     7201 cctgtgtccg gatacccgtc gggcgcgggg tgtgcggcac tgcggttgcc cgcaatcaag
     7261 tgcagcgtat cgaggatgtt cacgcgtttg acgggcatat tgcctgtgat gcggcgagta
     7321 attctgaaat tgttctgccg ctggtggtga aaaatcagat tattggtgtt cttgacatcg
     7381 atagtaccgt cttcggtcgc tttacagacg aggacgagca aggcttacgt cagcttgtgg
     7441 cacagcttga aaaagtgctt gcaacgacgg attacaaaaa attctttgcg agcgtcgcag
     7501 gataatcaac ggataacgta gcatttactg gtagcgtcat tataatgacg cctgttcatg
     7561 cctgcgcttg ttggctacgt ccgttgtaat caggaaattt catggaaaat caacctaagt
     7621 tgaatagcag taaagaagta atcgcgtttc tggccgaacg ttttccccac tgtttcagtg
     7681 cggaaggtga agcgcgtccg ctgaaaatcg gtatttttca ggatttggtc gatcgtgttg
     7741 ccggggaaat gaacctgagc aaaacgcaat tacgatccgc tttacgtctc tacacttcga
     7801 gctggcgtta tctttacggt gttaaacccg gcgcaacgcg tgtcgatctt gacggcaacc
     7861 catgcggtga gctggacgag caacatgtag agcatgctcg caagcagctt gaagaagcga
     7921 aagcgcgtgt tcaggcacag cgtgctgaac agcaagcgaa aaaacgcgaa gctgccgcag
     7981 ctgctggtga gaaagaagac gcaccgcgcc gcgaacgcaa gccacgtccg accacgccac
     8041 gccgcaaaga aggcgctgaa cgtaaacctc gtgcgcaaaa gccggtagag aaagcgccaa
     8101 aaacagtaaa agcacctcgc gaagaacagc acaccccagt ttctgacatt tcagctctga
     8161 ctgtcggaca agccctgaag gtgaaagcgg gtcaaaacgc gatggatgcc accgtattag
     8221 aaatcaccaa agacggcgtc cgcgtccagc tgaattcggg tatgtctttg attgtgcgcg
     8281 cagaacacct ggtgttctga aacggaggcc gggccaggca tgaacatgtt ttttaggctt
     8341 accgcgttag ctggcctgct tgcaatagca ggccagacct tcgctgtaga agatatcacg
     8401 cgtgctgatc aaattccggt attaaaggaa gagacgcagc atgcgacggt aagtgagcgc
     8461 gtaacgtcgc gcttcacccg ttctcattat cgccagttcg acctcgatca ggcattttcg
     8521 gccaaaatct ttgaccgcta cctgaatctg ctcgattaca gccacaacgt gctgctggca
     8581 agcgatgttg aacagttcgc gaaaaagaaa accgagttag gcgatgaact gcgttcaggc
     8641 aaactcgacg ttttctacga tctctacaat ctggcgcaaa agcgccgttt tgagcgttac
     8701 cagtacgctt tgtcggtact ggaaaagccg atggatttca ccggcaacga cacttataac
     8761 cttgaccgca gcaaagcgcc ctggccgaaa aacgaggctg agttgaacgc gctgtgggac
     8821 agtaaagtca aattcgacga gttaagcctg aagctggcag gaaaaacgga taaagaaatt
     8881 cgtgaaaccc tgacacgccg ctacaaattt gccattcgtc gtctggcgca aaccaacagc
     8941 gaagatgttt tctcgctggc aatgacggcg tttgcgcgtg aaatcgaccc gcataccaac
     9001 tatctttccc cgcgtaatac cgaacagttc aacactgaaa tgagtttgtc gctggaaggt
     9061 attggcgcag tgctgcaaat ggatgatgac tacaccgtta tcaattcgat ggtggcaggt
     9121 ggtccggcag cgaagagtaa agctatcagc gttggtgaca aaattgtcgg tgttggtcaa
     9181 acaggcaagc cgatggttga cgtgattggc tggcgtcttg atgatgtggt tgccttaatt
     9241 aaagggccga agggcagtaa agttcgtctg gaaattttac ctgctggtaa agggaccaag
     9301 acccgcactg taacattgac ccgtgaacgt attcgtctcg aagaccgcgc ggttaaaatg
     9361 tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg atattccggg cttctatgtg
     9421 ggtttgacag acgatgtcaa agttcaactg cagaaactgg aaaaacagaa tgtcagcagt
     9481 gtgatcatcg acctgcgtag caatggcggt ggggcgttga ccgaagcggt atcgctctca
     9541 ggtctgttta ttcctgcggg tcccattgtt caggtccgcg ataacaacgg caaggttcgt
     9601 gaagacagcg ataccgacgg gcaggtgttc tataaaggcc cgctggtggt actggttgac
     9661 cgtttcagtg cttcggcttc agaaatcttt gccgcggcaa tgcaggatta cggtcgtgcg
     9721 ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc agcaataccg ttcattgaac
     9781 cgtatttacg atcagatgtt acgtcctgaa tggccagcgc tgggttctgt gcagtacacg
     9841 atccagaaat tctatcgcgt taacggcggc agtacgcaac gtaaaggcgt aacgccagac
     9901 atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg agaaattcga agataacgcg
     9961 ctgccgtggg atagcattga tgccgcgact tatgtgaaat caggcgattt aacggccttt
    10021 gggccggagc tgctgaagga acataatgcg cgtatcgcga aagatcctga gttccagaac
    10081 attatgaagg atatcgcgcg cttcaacgct atgaaggaca agcgcaatat cgtttctctg
    10141 aactacgctg tgcgtgagaa agagaataat gaagatgatg cgacgcgtct ggcgcgtttg
    10201 aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga agctggatga tctaccgaaa
    10261 gattaccagg agccggatcc ttatctggat gagacggtga atatcgcact cgatctggcg
    10321 aagcttgaaa aagccagacc cgcggaacaa cccgatcccg tcaagtaata tcaatcaggc
    10381 acaagaaatt gtgcctgatt ttttaacagc ggcaagatgc cgtaaatcag atgctacaaa
    10441 atgtaaagtt gtgtctttct ggtgacttac gcactatcca gacttgaaaa tagtcgcgta
    10501 acccatacga tgtgggtatc gcatattgcg ttttgttaaa ctgaggtaaa aagaaaatta
    10561 tgatgcgaat cgcgctcttc ctgctaacga acctggccgt aatggtcgtt ttcgggctgg
    10621 tactgagcct gacagggata cagtcgagca gcgttcaggg gctgatgatc atggccttgc
    10681 tgttcggttt tggtggttcc ttcgtttcgc ttctgatgtc caaatggatg gcattacgat
    10741 ctgttggcgg ggaagtgatc gagcaaccgc gtaacgaaag ggaacgttgg ctggtcaata
    10801 ctgtagcaac ccaggctcgt caggcaggga tcgctatgcc gcaagtggct atctaccatg
    10861 cgccggacat caacgctttt gcaaccggtg cgcgccgtga tgcctctctg gttgctgtca
    10921 gcaccggttt gctgcagaac atgagcccgg atgaagccga ggcggtaatt gctcacgaaa
    10981 tcagtcacat cgccaatggt gatatggtta ccatgacgct gattcagggc gtggtgaaca
    11041 ccttcgttat ctttatttcc cgtattctgg cgcagcttgc cgcgggtttt atgggcggaa
    11101 atcgtgatga aggtgaagag agcaacggca acccgctgat ctactttgcg gttgcaacgg
    11161 ttctggaact ggtgtttggt attctggcga gcattatcac catgtggttc tcgcgtcatc
    11221 gtgaattcca tgctgatgcc ggttcggcaa aactggttgg tcgcgagaaa atgattgccg
    11281 cgctgcagcg cctgaaaacc agctatgaac cgcaagaagc aaccagcatg atggctttct
    11341 gcattaacgg taagtcgaaa tcgctcagtg agttgttcat gactcacccg ccgctggata
    11401 aacgaattga agctctgcgt acgggtgaat acctgaagta atatgaagta aatactaaaa
    11461 ggcgcgtcct gatcgacgcg ctttttttat gccctggatc gtggctgagt gatacgtaaa
    11521 ccactcacac aggcagcaat cactgccaga atagccgcag ccatcagcga aacgtgcgta
    11581 ccattatcac cgaactgatt tagcatcagc gccaccagcg ccgcgccact actctgaccc
    11641 agtagacgag ccgttcctaa catgccactg gccccaccgc tacgttcgcg cggagcggag
    11701 gtaataatgg tgtggttatt gggtgactgg aataagccaa atccagcgcc acataagatc
    11761 atcggccaga taatattgat atccgcaggt gacgcgggca gcagaaccag ggaaaaaagc
    11821 cccgcagcca tgatgaacaa ccctaaagca cccagcaatc ctgcatgtac gcgttcaatc
    11881 aaatagcctg ccagcggagc catcaccatc gttgctaacg gccacggttt cagaagtaaa
    11941 cctgtttcga cttcactacg cccgagcacg gtttgcaggt aaaagggcag ggaaaccatt
    12001 gccagcattt gtgcgcagaa agagcaaaca gatgtgcaaa tagaaagtga aaacagcggg
    12061 atacgcagta aatccaccgg tagcagcggt acgggaagag aaagctggcg gcgaataaag
    12121 aaaataccaa caacaaccat taccaccagt tccgcagcaa ttaacgtcag cgattgcccc
    12181 tgagcgaaac cactcaacgc agtgataagc aggccgaagg ttaacgcgtt catcacggcg
    12241 ctgggcaggt cgaaacgggg tttactggcg cgagaaccat tgggtggcag aaaacgcatc
    12301 gccagaagca gggcgataat acctaacggt acgttgatta aaaataacca tttccaggat
    12361 gcgatggaga ggattgctgc agcaattgtc ggcccggcag cagaagaaac ggcaacaata
    12421 aacgagttta tgcccatccc tctccccaga aaacgttgtg gatagatcag gcggataagt
    12481 gcggtattaa cgctcatcaa cgctgcaccg ccgaaacctt gtatgacacg cgcaagggtg
    12541 agcatttgca gcgaatcaga aagggcgcag aacagtgaag acaacagaaa aacgaccaga
    12601 ccgcatttat aaatacgtcg atagccaaac atatcgccca gaaacgaaaa cgagagcagg
    12661 gagatgacaa tggcgatttg ataggcgttc actacccaga tggatctggc tggcgtggca
    12721 tgaaggtccg tggcgattgt tggcagggcg acgttggcga ttgcgccgtc aaggacagcc
    12781 atcgaaatgc caatcacaat ggttaatatc gcaccgtatc gctggggcaa tggcaggccg
    12841 tcggcctgaa cttttggcat aggaatttta tatctttggt gaataatcaa caggggtatg
    12901 attctagcac tgatattcac ggtggatgtc gcagaattgt aactaaccgg caaaagatgg
    12961 attgcgacca ccctggtgcg aaattataat aaaaaacagt tctgattttt ataaaacact
    13021 cgcaatgagg tgataaatgg ctaacgcaga tctggataaa cagcctgatt ctgtatcttc
    13081 cgtgctaaaa gtttttggca ttttgcaggc gctgggtgaa gagcgcgaaa tagggataac
    13141 cgagctgtcg cagcgcgtca tgatgtcaaa aagcaccgtt tatcgctttt tacagaccat
    13201 gaaaacctta ggttatgtgg cgcaggaagg ggagtcggag aaatattccc tgaccctgaa
    13261 attgtttgaa ctgggcgctc gcgcgttaca aaacgtcgat ttaattcgta gcgcagatat
    13321 ccagatgcgt gagatctccc gcctgaccaa agaaactatc cacctcggcg cactggacga
    13381 agacagtatt gtttacattc acaaaattga ctctatgtac aatttgcgca tgtattcacg
    13441 gattgggcgt cgtaatccgc tgtacagcac cgcgattggt aaggtactgc tggcatggcg
    13501 cgatctcgat gaagtgaagc aaattcttga gggcgtggag tataaacgca gtaccgagcg
    13561 gaccatcacc agtacagaag cgttattacc cgttctggac caggtgcgcg agcaggggta
    13621 tggcgaagat aacgaagagc aggaagaagg gctgcgatgc attgcggtac cggtatttga
    13681 tcgctttggc gtggtcattg ccggtttgag catctccttc ccgacgttgc gtttctctga
    13741 agagcgttta caggaatatg tcgcaatgtt gcataccgca gcgcgcaaaa tttctgccca
    13801 aatgggttat cacgactatc cgttctgatg agagtaagaa cctgtcggaa tatcaaacag
    13861 acaggttctt tatttagcat gagaaaaata aagttgaagg tgcgttatat taaacgcgct
    13921 tgctataaga atattttact catgagtgag aatctggtta tttattaccc ttaaccatta
    13981 tcgaccacga tattgctttt acgtaacagt gggcaatctg ttatccccaa gaaaccactt
    14041 ttagtgtgca agtattgtac cgtgctggtg cctctggcag tcagataggt acattgcaaa
    14101 cctaatcctg cggcattctc tttgcttcca atcaaaacgc catatccgct gagtaataat
    14161 cctatccata ccagtgctat cagcataact gtgcgaatga tgaatcgcat tacaacctct
    14221 tctcttttta tgttcgctta atcgtagcgg caatatgcgc tgaagcaagc tactcattcc
    14281 gaaaaagcac gaatatcgac atagttaggc gctgtttaac taacgcatgc tagtttaatg
    14341 acataaggta ggtgaagcgg agattggagt gaaaaagttt cgatgggtcg ttctggttgt
    14401 cgtggtgttg gcttgcttgc tgctttgggc gcaggtattc aacatgatgt gcgatcagga
    14461 tgtacaattt ttcagcggaa tttgtgccat taaccagttt atcccgtggt gatatcattt
    14521 tttcagcact agattttctt cctctccagc agtggtagaa tagcgcctct actcagaggt
    14581 ggtgaaatga acgaagttgt aaattcaggc gtgatgaaca ttgcgtcttt ggttgtatcg
    14641 gtggtggttc ttcttatcgg gctcatcttg tggtttttta tcaatcgtgc cagttctcgg
    14701 actaacgaac agattgaact gcttgaggcg ttgctggatc agcaaaaacg tcaaaatgca
    14761 ctgttacgtc gtttgtgcga agcaaacgaa cctgagaaag cagataaaaa gaccgttgag
    14821 agtcaaaaat cggttgaaga cgaagatatt attcgtctgg tcgccgaacg ataatccttc
    14881 ctcaatcttg agtaaccccg tcgttgaagg gttactcaat tcatcctgat tatacgcact
    14941 gccatggtgc aactgcttca aattaaaact ct
//
LOCUS       DAZFRJ010000113        14958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000113 DAZFRJ010000000
VERSION     DAZFRJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.114"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..1352
                     /gene="hpaG"
                     /locus_tag="ACHKUN_003084"
     CDS             63..1352
                     /gene="hpaG"
                     /locus_tag="ACHKUN_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO6661300.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1349..2815
                     /gene="hpaE"
                     /locus_tag="ACHKUN_003085"
     CDS             1349..2815
                     /gene="hpaE"
                     /locus_tag="ACHKUN_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6661301.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2817..3668
                     /gene="hpaD"
                     /locus_tag="ACHKUN_003086"
     CDS             2817..3668
                     /gene="hpaD"
                     /locus_tag="ACHKUN_003086"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO6661302.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3678..4058
                     /locus_tag="ACHKUN_003087"
     CDS             3678..4058
                     /locus_tag="ACHKUN_003087"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO6661303.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4169..4972
                     /gene="hpaH"
                     /locus_tag="ACHKUN_003088"
     CDS             4169..4972
                     /gene="hpaH"
                     /locus_tag="ACHKUN_003088"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO6661304.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4983..5771
                     /gene="hpaI"
                     /locus_tag="ACHKUN_003089"
     CDS             4983..5771
                     /gene="hpaI"
                     /locus_tag="ACHKUN_003089"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO6661305.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5946..7322
                     /gene="hpaX"
                     /locus_tag="ACHKUN_003090"
     CDS             5946..7322
                     /gene="hpaX"
                     /locus_tag="ACHKUN_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO6661306.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7332..8222
                     /gene="hpaA"
                     /locus_tag="ACHKUN_003091"
     CDS             7332..8222
                     /gene="hpaA"
                     /locus_tag="ACHKUN_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO6661307.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8473..10035
                     /gene="hpaB"
                     /locus_tag="ACHKUN_003092"
     CDS             8473..10035
                     /gene="hpaB"
                     /locus_tag="ACHKUN_003092"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO6661308.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10053..10565
                     /locus_tag="ACHKUN_003093"
     CDS             10053..10565
                     /locus_tag="ACHKUN_003093"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO6661309.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10958..13108
                     /gene="btsT"
                     /locus_tag="ACHKUN_003094"
     CDS             10958..13108
                     /gene="btsT"
                     /locus_tag="ACHKUN_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO6661310.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13239..13442
                     /locus_tag="ACHKUN_003095"
     CDS             13239..13442
                     /locus_tag="ACHKUN_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6661311.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13453..14409
                     /gene="yjiA"
                     /locus_tag="ACHKUN_003096"
     CDS             13453..14409
                     /gene="yjiA"
                     /locus_tag="ACHKUN_003096"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6661312.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14496..14687)
                     /locus_tag="ACHKUN_003097"
     CDS             complement(14496..14687)
                     /locus_tag="ACHKUN_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO6661313.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14727..>14958)
                     /locus_tag="ACHKUN_003098"
     CDS             complement(14727..>14958)
                     /locus_tag="ACHKUN_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane domain protein"
                     /protein_id="HFO6661314.1"
                     /translation="STIDFEWRNQVKCGELNISRPDERYFGFNTDKYTVFYSNREGKW
                     GVNELKCKKGSDRRDAYSIENVSEYNVPGWLK"
BASE COUNT         3468 a         4103 c         4086 g         3301 t
ORIGIN      
        1 tcgggaataa aagcaattat taacatatta atgattaaga tgatcgaatc cgaggagtgg
       61 taatgaaagg cactatcttc gccgtagcgt tgaaccatcg cagccaactt gatgcatggc
      121 aggaagcgtt ccagcaatcc ccctacaaag ccccgcctaa aactgcggtc tggtttatta
      181 aaccgcgcaa tacggtgatt ggttgcggtg aaccgattcc ctttccacag ggtgaaaagg
      241 tactgagcgg tgcgactgtt gcgctgattg tgggaaaaac ggcgacgaaa gtacgtgaag
      301 aagatgcggc agagtacatc gccggatatg cgctggctaa cgacgtcagc ctgccggaag
      361 agagctttta ccgcccggca atcaaagcaa aatgtcgtga tggattctgc cccattggcg
      421 aaaccgtggc tctcagcaat gtcgataatc tgaccatcta taccgagatc aacgggcgtc
      481 ctgccgatca ctggaatacc gccgatttac aacgtaacgc cgcgcagttg ctgagcgccc
      541 tgagcgaatt tgccacactg aatccaggcg atgccattct gctcggcacg ccacaggcgc
      601 gcgtggaaat acagccaggc gatcgcgttc gtgttctcgc agaaggtttc ccgccgctgg
      661 aaaatccggt agtggacgaa cgtgaagtga ccacgcgcaa gagcttccca acgcagccac
      721 acccgcacgg cacgctgttt gccctcggcc tgaactacgc cgaccacgcc agcgaactgg
      781 aatttaagcc accggaagaa ccgctggtgt tcctgaaagc gccaaatacc ctcactggcg
      841 ataaccagac ctccgtgcgt ccaaacaata ttgaatacat gcactatgaa gcggagctgg
      901 tggtagttat tggcaaacag gcgcgtaacg tcagcgaagc cgatgccatg gattatgtcg
      961 cgggctacac cgtgtgtaac gactacgcca ttcgcgacta tctggaaaac tactaccgcc
     1021 ctaacctgcg ggtaaaaagc cgcgacggac tgacgccgat gctttcaacc atcgtgccga
     1081 aagaggcgat cccggacccg cataatctga cccttcgcac cttcgtcaac ggcgagttac
     1141 gccagcaagg caccaccgcc gatctgatct tcagcgtgcc cttcctgatc gcctacttaa
     1201 gcgaatttat gaccctgaat ccgggcgaca tgatcgccac cggcacacca aaaggcttat
     1261 ctgacgtggt gcctggcgat gaagtagtgg tggaagtaga aggcgtgggc tgcctggtga
     1321 accgaattgt gagtgaggaa acagcgaaat gaaaaaagta aatcattgga tcaacggcaa
     1381 aaatgttgca ggtaacgact acttccagac caccaatccg gcaacgggtg aagtgctggc
     1441 ggatgtggcc tctggcggtg aagcggagat caatcaggcg gtagcggcag cgaaagaggc
     1501 gttcccgaaa tgggccaatc tgccgatgaa agagcgtgcg cgcctgatgc gccgtctggg
     1561 cgatctgatc gaccagaacg tgccagagat cgccgcgatg gaaaccgcgg acaccggcct
     1621 gccgatccat cagaccaaaa atgtgttgat cccacgcgct tcccacaact ttgaattttt
     1681 cgcggaagtc tgccagcaga tgaacggcaa gacctatccg gttgacgaca agatgctcaa
     1741 ctacacgctg gtgcagccgg tgggcgtttg tgcgctggta tcgccgtgga acgtaccgtt
     1801 tatgaccgcc acatggaagg tcgcgccgtg tctggcgctg ggcaatactg cggtgctgaa
     1861 gatgtcagaa ctctccccgc tgaccgctga ccgcctgggt gagctggcgc tggaagccgg
     1921 tattccggcg ggcgtgctga acgtggtaca gggctacggc gcaaccgcag gggatgcgct
     1981 ggtccgtcat catgacgtgc gtgccgtgtc gttcaccggc ggtaccgcca ccgggcgcaa
     2041 catcatgaaa aacgccgggc tgaaaaaata ctccatggaa ctgggcggaa aatcgccagt
     2101 gctgattttt gaagatgccg atattgaacg cgcgctggac gccgccctgt tcaccatctt
     2161 ctcgatcaac ggcgagcgct gcaccgccgg ttcgcgcatc tttattcagc aaagcatcta
     2221 cccggaattc gttaaacgct ttgccgaacg cgccaaccgt ctgcgcgtgg gcgatccgaa
     2281 cgatccgaat acccaggttg gggcacttat cagccagcaa cactgggaaa aagtctccgg
     2341 ctatatccgt ctcggcattg aagaaggcgc aaccctgctg gcgggcggcc cggataaacc
     2401 gtctgacctg cctgcgcacc tgaaaggcgg caacttcctg cgcccaacgg tgctggcaga
     2461 cgtagataac cgtatgcgcg tcgcccagga agagattttc gggccggtcg cctgcctgct
     2521 gccgtttaaa gacgaagccg aaggcttacg cctggcaaac gacgtggagt acggcctcgc
     2581 gtcgtacatc tggacacagg atgtcagcaa agtgttacgc ctggcgcgtg gcattgaagc
     2641 aggcatggtg ttcgtcaata cccagaacgt ccgcgacctg cgccagccgt ttggcggcgt
     2701 aaaagcctcc ggcaccgggc gtgaaggcgg tgagtacagc ttcgaagtgt tcgcggaaat
     2761 gaagaacgtc tgcatttcca tgggcgacca tccaattccg aaatggggag tctgatatgg
     2821 gtaagttagc gttagccgcc aagatcacgc acgtaccgtc gatgtatctc tctgaactac
     2881 caggaaaaaa ccacggttgt cgccagggtg cgatcgacgg gcataaagag atcagcaagc
     2941 gttgccggga aatgggcgtc gataccatta tcgttttcga cacccactgg ctggtcaaca
     3001 gtgcttatca catcaactgt gcagaccatt ttgaaggcgt ctacaccagt aacgaactgc
     3061 cgcattttat tcgtgacatg acctacaact acgagggcaa cccggagttg gggcagctaa
     3121 ttgccgatga agccttaaag ctcggcgtgc gggcaaaagc gcacaatatt cccagcctga
     3181 aactggaata cggcacgctg gtgccgatgc gctacatgaa tgaagacaag cacttcaaag
     3241 tggtctccat ttcggctttc tgcacggttc acgattttgc cgacagccgc aagctgggcg
     3301 aagcgattct gaaagcgatc gaacagtacg acggcaccgt ggcggtcctt gccagcggtt
     3361 cgttatcgca ccgctttatt gacgatcagc gtgcggaaga agggatgaac agctacaccc
     3421 gcgagttcga ccgccagatg gacgagcgcg tggtgaagct gtggcgcgaa ggccagttca
     3481 aagagttttg caatatgctg ccggagtacg ccgactactg ctacggcgaa ggcaatatgc
     3541 acgacacggt gatgctgctg gggatgctcg gctgggataa atacgacggc aaggtggagt
     3601 ttatcaccga gctattccca agctctggca ccggtcaggt taacgctgtt ttcccgcttc
     3661 ccgcgtaagg acgttttatg ccgcacttta tcgttgaatg cagtgataac atccgcgaag
     3721 aagccgacct gccggggttg ttcgccaaag tgaatccgac gctggcagcc acgggtattt
     3781 ttccgctggc gggtattcgc agccgcgtgc attgggtcga tacctggcag atggccgacg
     3841 ggcagcatga ttatgccttc gtgcatatga cgttgaaaat cggcgcaggt cgcagcctgg
     3901 aaagccgcca gcaggcgggt gaaatgctgt ttgaactgat taaaacgcac ttcgccgccc
     3961 tgatggagag ccgcctgctg gcgttgtcgt ttgagattga agagctgcat ccgacgctga
     4021 attttaaaca aaacaacgtg cacgcattgt ttaagtagcg cgcagattgc ccggtggcgc
     4081 tgcgcttacc gggcctacaa aaccccaaac cgtacaccgt aggccggata aggcgcagcc
     4141 gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca ccctgatcgc
     4201 ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct cgctggatta
     4261 cccggagatc accatcgaag acgcttacgc ggtgcagcgt gaatgggttc gcctgaaaat
     4321 cgccgaaggt cgcacgctga aaggccacaa aatcggcctg acctcgaaag cgatgcaggc
     4381 aagctcgcag atcagcgaac cggattacgg cgcgctgctg gatgacatgt tcttccacga
     4441 tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg agctggcttt
     4501 cgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgtct acaacgccac
     4561 ggactacgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca tcgatccgga
     4621 aacccagcgc ccgcgcaaag ttttcgacac catttctgat aacgccgcca acgccggggt
     4681 gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga tctccgccct
     4741 gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggcgtgc tgaatcatcc
     4801 ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac aactggaagc
     4861 cgggcaaatc attctcggtg gttcgttcac ccgcccggtt ccggcgcgta agggcgacac
     4921 cttccacgtc gattacggca acatgggttc cattagctgc cgctttgttt aaggagagaa
     4981 cgatggaaaa cagctttaaa gcggcgctga aagcaggccg tccgcagatc ggattatggc
     5041 tggggctgag tagcagctac agcgcagagt tactggccgg agcaggattc gactggttgt
     5101 tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccag ctacaggcga
     5161 ttgcgcccta ccccagccag ccggtagtac gtccgtcgtg gaacgatccg gtgcaaatca
     5221 aacaactgct ggacgtcggc acacaaacct tgctggtgcc gatggtacaa aacgctgacg
     5281 aagcccgtga agcggtacgc gccacccgtt atccccccgc cggtattcgc ggtgtgggca
     5341 gtgcgctggc ccgagcctcg cgctggaatc gcattcctga ttacctgcaa aaagccaacg
     5401 atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac ttaccgcaga
     5461 ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg agcgccgata
     5521 tgggttatgc cggtaatcct cagcacccgg aagtacaggc cgcgattgag caggcgatcg
     5581 tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag caactggcaa
     5641 aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc accctgctcg
     5701 cccgcgccgc cgaagcgctg gcagcacggt ttggtgcgca ggctacagcg attaagcccg
     5761 acgtgtatta atgccggatg cgctgcgctt atccggccta cactcgcacc aaaccgtagg
     5821 ccggataagg cgtttacgcc gcatccggca aaaagccgta ccaaatcgcg gaccggataa
     5881 ggcgtttgtg cgcatccgcc ataaaccttg tcgtacccta caaaaatccc attagaggaa
     5941 gtaaaatgag cgacacctca cctgccatac cggagagtat cgatccggcg aatcagcata
     6001 aagcgctgac tgccggacaa caggcggtta ttaagaagct atttcgccgc ctgatcgtct
     6061 ttctgttcgt gctgtttatc ttctcgttcc ttgatcgcat caacatcggc tttgccggac
     6121 tcacgatggg acgcgacctc ggtctgagcg ccaccatgtt tggcctcgct accaccttgt
     6181 tctacgccgc ttacgtcatc ttcggcattc ccagcaacat tatgctgagt attgtcggtg
     6241 cacggcgctg gatcgccacc atcatggtgc tttggggcat cgcctctact gccaccatgt
     6301 ttgccactgg ccccaccagc ttatacgtac tgcgtatact ggttggcatt accgaagccg
     6361 gctttctgcc tggcattctg ctgtatttaa ccttctggtt tccggcctat ttccgcgccc
     6421 gtgccaacgc cttgtttatg gtagcaatgc cggtaacgac agcgttggga tcgatcgttt
     6481 ccggctacat tttgtcgctg gatggcgtaa tggcattaaa aggctggcag tggctgtttt
     6541 tgctggaagg cttcccgtcg gtattactcg gcgtcatggt gtggttctgg cttgatgact
     6601 caccggacaa agctaagtgg ctgacgaaag aagacaaaaa atgcctgcaa gagatgatgg
     6661 ataacgatcg tctgacgctg gttcagccag agggagccat cagccaccac gccatgcaac
     6721 aacgcagtat gtggcgggag atcttcactc cggtggtgat gatgtatacc ctggcgtatt
     6781 tctgcctgac caacacactt agtgcgatca gcatctggac accgcagatc ctgcaaagct
     6841 ttaatcaggg cagcagtaat atcaccatcg gcctgctggc cgccgtaccg cagatttgta
     6901 ccattctcgg gatgatctac tggagccgtc actcagatcg ccgccaggaa cgaaggcatc
     6961 acaccgccct tccttatttg ttcgctgccg ctggttggtt actggcttcg gcaactgatc
     7021 acaacatgat ccagatgctg gggatcatta tggcttcgac cggatcattc agcgcaatgg
     7081 cgattttctg gacaacaccg gatcagtcca tcagcctgcg ggcacgagcg atcggtattg
     7141 cggtgatcaa cgccactggc aacattggtt cagcgttaag tccgtttatg atcggctggt
     7201 tgaaagatct gaccggcagc tttaacagtg gattgtggtt tgttgccacg ctgctggtga
     7261 ttggtgcggg gattatctgg gcgattccaa tgcagtcctc ccgtccgcga gcgaccccgt
     7321 aaggaacgac gatgtgtgac cgtcagattg ccaatattga tatcagcaaa gagtacgatg
     7381 aaagcctggg cacggacgat gtgcattatc agtcgttcgc ccgcatggcg gccttttttg
     7441 gccgccatat gctgccacat cgccacgaac agtactttca gatgcatttc ctcaatagcg
     7501 gacagattga gctacagctt gacgatcatc gctactcggt ggaagcgccc ctgtttgtcc
     7561 tgacgccgcc gtcagtacct catgcgttta ttacggagtc tgatgctgac ggtcatgtat
     7621 tgacggtacg ggaagatctg atctggcccc tgctggaagt tctttatccg ggcactcggg
     7681 aaaccttcgg cctgccgggg atttgcctgt cactggcaga taaacccgac gaactggcgg
     7741 cgctggaaca ctattggcaa ctgatagagc gggaatcggt agaacaactg cctggtcggg
     7801 aacacaccct gacgttactg gcacaggcag tgttcaccct actgctgcgt aacgcaaaac
     7861 tcgacgacca tgccgccagc ggaatgcgcg gagaattaaa actgttccag cgttttcata
     7921 tgctgattga aagccatttt catcagcact ggacagtacc ggattacgct aacgaattgc
     7981 atatcaccga atcacgcctc actgacatct gccgccgctt tgccaaccgt ccgccaaaac
     8041 ggttgatttt cgacaggcag ctgcgagaag ccaagcggct gctgctgttt tctgataacg
     8101 ccgtgaacaa tattgcctgg caactcggtt ttaaggatcc ggcttatttt gcgcgctttt
     8161 ttaatcgctt agtcggttgc tcgcccagtg cttatcgcgc caaaaaagta cctgtgacgt
     8221 gaaaatccct tagccttaac gggaaaccag gcaccacctg ctattcccct ttctccgcct
     8281 ggagcaaggg gatttagtaa cgttaaaaat cgctaaaagc gacctcgatc acaaaaccgt
     8341 acccgatctg aaaagtacca gccattgtcc caaaagtgtc ttcccgtaat cgcccctgca
     8401 agcttcaatt gaataacaaa aacaacacac aaaattaaca atcgtattcc gattaatact
     8461 gtagaggtcg acatgaaacc agaagatttc cgcgccagta cccaacgtcc gttcaccggg
     8521 gaagagtatc tgaaaagcct gcaggatggt cgcgagatct atatctatgg cgagcgagtg
     8581 aaagacgtca ccactcatcc ggcatttcgt aatgcggcag cgtctgttgc ccaactgtac
     8641 gacgcgctac acaaaccgga gatgcaggac tctctgtgct ggaacaccga caccggtagc
     8701 ggcggctata cccataaatt cttccgcgtg gcgaaaagtg ccgacgacct gcgccagcaa
     8761 cgcgacgcca tcgctgagtg gtcacgcctg agctatggct ggatgggccg taccccagac
     8821 tacaaagccg ctttcggttg cgcactgggc gcgaatccgg gcttttacgg tcagttcgag
     8881 cagaacgccc gtaactggta tacccgtatt caggaaactg gcctctactt taaccacgcg
     8941 attgttaacc caccgatcga tcgtcatttg ccgaccgata aagtgaaaga cgtttacatc
     9001 aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt ggttgccacc
     9061 aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt gatgggcgaa
     9121 aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt gaaattaatc
     9181 tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccgtacga ctacccgctc
     9241 tccagccgct ttgatgagaa tgatgcgatt ctggtgatgg ataacgtgct gatcccatgg
     9301 gaaaacgtac tgatctaccg cgatttcgat cgctgccgtc gctggacgat ggaaggcggt
     9361 tttgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact agacttcatt
     9421 acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg tggtgtgcag
     9481 gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag tgactcgatg
     9541 tgttcagaag cgacaccatg ggtcaacggt gcttatttac cggatcatgc cgcactgcaa
     9601 acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat cgaacgcaac
     9661 gttaccagtg gcctgatcta tctcccttcc agtgcccgtg acctgaacaa tccgcagatc
     9721 gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt ccagcgcatc
     9781 aagatcctca aactgatgtg ggacgccatt ggcagcgagt ttggtggtcg tcacgaactg
     9841 tatgaaatca actactccgg tagccaggat gagattcgcc tgcagtgtct gcgccaggca
     9901 caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct gtcggaatac
     9961 gaccagaacg gctggactgt gccgcacctg cacaacaacg acgatatcaa catgctggat
    10021 aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa cgcctgcgct
    10081 ttcgtgacgc aatggccagc ctgtcggcag cggtaaatat tatcaccacc gagggcgacg
    10141 ccggacaatg cggaattacg gcaacggccg tctgctcgat cacggataca ccaccgtcgc
    10201 tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc aacggtaagt
    10261 tgtgcgtcaa cgtcctcaat catgagcagg agctgatggc ccgccacttc gcgggcatga
    10321 caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt ccgctggcac
    10381 agccggtgct gaaaggttcg ctggccagtc ttgaaggtga gatccgcgat gtgcaggcaa
    10441 ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt gcagaaggtc
    10501 acggacttat ctactttaaa cgccgtttcc atccggtgat gctggaaatg gaagctgcga
    10561 tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg atctcaatca
    10621 catatccacc cgatgaataa ttcttaccca accgctcacc gccattcctg accgcttaca
    10681 tccctaaaat aaccactcag ttatttacct tactttacgc gtgcgtaact ctggcaacat
    10741 cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag aacggcttcg
    10801 gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc aagtgagtga
    10861 atccccggga gcttataaca gtaagtgaca ggggtgaacg aacgcagctg ccgcacctgt
    10921 aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa aactattcaa
    10981 gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt
    11041 acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct
    11101 ggtggcgtat cgctactaca gtctgtatat cgcccagaag gtgatgaaac tcgaccccac
    11161 gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga ccaaccgtta
    11221 cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg ttggtccggt
    11281 tctcgccgcg cagatgggct acctgcccgg cacgctgtgg ctgctggcgg gggtagtgct
    11341 ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta acggcgcatc
    11401 tctcggtgag atgatcaaag aagagatggg accagtaccg gggactatcg cgctgtttgg
    11461 ctgtttctta atcatgatca tcatccttgc cgtcctggcg ctgattgtgg ttaaagccct
    11521 ggcagaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat
    11581 gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg taatcggtat
    11641 cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc cgtactgggg
    11701 tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct atgcgtttgt
    11761 ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg caaccttcct
    11821 gaaaatcggc gttatcgtcg gcctggcgtt gggtatcgtg gtgctgaacc cggaactgaa
    11881 aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag gcgctctgtt
    11941 cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg cgctgatctc
    12001 ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca ttggctacgg
    12061 cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt ccatcatcga
    12121 accgggtctt tacttcgcga tgaacacccc gcctgcgggc cttggcatca ccatgcctaa
    12181 cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga aagacgttac
    12241 cgcacacgcg gcggcgaccg tcagctcctg gggcttcgtg atttcgccag agcagatcct
    12301 gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccgac
    12361 actggcggta ggtatcgcgc acgtgttcca caaagtgctg ccgatggctg acatgggctt
    12421 ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg
    12481 tacccgttct ggccgcttta tgctgcagga cctgctgggt aacttcatcc cgttcctgaa
    12541 aaaaaccgac tctctggttg ctggtatcat cggtactgcg ggctgtgtgg gtctgtgggg
    12601 ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc tgtggccgct
    12661 gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca ccgttgtgct
    12721 gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg tatggctgct
    12781 tatctgcacc acctgggcgc tgggcctgaa actgttcagc accaacccgc agatggaagg
    12841 cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg acctgacggc
    12901 acagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg cgggcctgag
    12961 tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa cctggcttgc
    13021 ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa tcccggaagg
    13081 cggcgtgaag atctcttcgc accactaacc ggttgccgga tgcgttgcgc gtatccggcc
    13141 tacaaagtgt ctatcgtagg ccggataaag cgtttacgcc gcatccggca aatccctagc
    13201 ccggaccttc cgggctttac gcatatctgg aaggcaaaat gtttggtaac ttaggacagg
    13261 caaaaaaata tctcggccag gcggcgaaga tgttgattgg cattccagac tacgacaact
    13321 acgtcgagca tatgaagacc aaccatcccg acaagccgta catgagctat gaagaattct
    13381 tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt
    13441 aatggagaga gcatgaaccc gattgcagtt accctactca ccggtttttt aggcgcgggc
    13501 aaaaccaccc tgctgcgcca tattcttaac gaacaacacg gctacaagat tgccgtgatt
    13561 gaaaacgaat tcggcgaagt ctccgttgat gatcaactga ttggcgaccg cgccacgcag
    13621 atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct ggaagacgca
    13681 ctactcgacc tgctggataa tctcgacaag ggcaatattc agttcgaccg tctggtcatt
    13741 gaatgcaccg gcatggccga tcccggtccg attattcaga cctttttctc ccatgagatt
    13801 ttatgccagc gttacctgct ggacggcgtg attgcgctgg tggatgcggt acatgccgat
    13861 gagcagatga accagttcac catcgcccag tcccaggttg gctacgccga ccgtattctg
    13921 ctgaccaaaa ccgacgtcgc aggcgaagca gaaaaactgc gtgaacgcct ggcgcgcatc
    13981 aacgcccgtg caccggtcta caccgtcacc cacggcgaca tcgacctggg cctgctattc
    14041 aacaccaacg gttttatgct ggaagagaac gtcgtcagca ccaaaccgcg cttccacttt
    14101 atcgcggata aacaaaacga tatttcgtcg attgtggtgg aactggatta cccggtagat
    14161 atcagcgaag tttcccgggt gatggaaaac ctgctgctgg aatcggcaga taaactgctg
    14221 cgttacaaag ggatgctgtg gattgacggc gaacctaacc gcctgctatt ccagggcgtc
    14281 cagcgcctct acagcgccga ctgggaccgc ccgtggggcg atgaaaaacc gcatagcacg
    14341 atggtgttta tcgggattca gctgccggag gaagagattc gggcggcgtt tgcggggttg
    14401 aggaagtaat cgtgaaaggt tgagtgtaaa gcagctcaat gccccattta gcagtgaggg
    14461 gggaaacaga acgacgtgct cccctccgct aagttttatt gcgcgtgaat acctggatct
    14521 ggcaaaaaat tgccgcgttt ggcgtcagac catgcttcgc taccagaagc aggaatgtcg
    14581 ctgtaatcaa tgtcttcgtc ggatttatta accaaagcca tgagttcagc ttcttgctgc
    14641 acatcaagtg tgtagagtgt gccccgttta tgtttaacca tgctcattta tgaccagaac
    14701 ctttacgatt taattataaa gatgacttat ttcagccatc caggcacgtt atattctgag
    14761 acattctcaa tggaatatgc atctctcctg tcagaacctt tcttacactt caactcattg
    14821 accccccatt taccctctct attggaataa aatacagtat atttatcggt attaaacccg
    14881 aaatatcttt catccggacg tgaaatattc aactctccac atttaacctg attacgccat
    14941 tcaaaatcta tggtgctt
//
LOCUS       DAZFRJ010000114        14722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000114 DAZFRJ010000000
VERSION     DAZFRJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.115"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(41..193)
                     /gene="hokE"
                     /locus_tag="ACHKUN_003099"
     CDS             complement(41..193)
                     /gene="hokE"
                     /locus_tag="ACHKUN_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6661315.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            162..431
                     /locus_tag="ACHKUN_003100"
     CDS             162..431
                     /locus_tag="ACHKUN_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661316.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            635..1753
                     /locus_tag="ACHKUN_003101"
     CDS             635..1753
                     /locus_tag="ACHKUN_003101"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO6661317.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1819..2067
                     /locus_tag="ACHKUN_003102"
     CDS             1819..2067
                     /locus_tag="ACHKUN_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO6661318.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            2132..2500
                     /locus_tag="ACHKUN_003103"
     CDS             2132..2500
                     /locus_tag="ACHKUN_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6661319.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2594..3247
                     /gene="nfsB"
                     /locus_tag="ACHKUN_003104"
     CDS             2594..3247
                     /gene="nfsB"
                     /locus_tag="ACHKUN_003104"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO6661320.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3355..4602
                     /gene="ybdG"
                     /locus_tag="ACHKUN_003105"
     CDS             3355..4602
                     /gene="ybdG"
                     /locus_tag="ACHKUN_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO6661321.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4670..6046)
                     /gene="pheP"
                     /locus_tag="ACHKUN_003106"
     CDS             complement(4670..6046)
                     /gene="pheP"
                     /locus_tag="ACHKUN_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO6661322.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6148..9291)
                     /gene="cusA"
                     /locus_tag="ACHKUN_003107"
     CDS             complement(6148..9291)
                     /gene="cusA"
                     /locus_tag="ACHKUN_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO6661323.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9303..10526)
                     /gene="cusB"
                     /locus_tag="ACHKUN_003108"
     CDS             complement(9303..10526)
                     /gene="cusB"
                     /locus_tag="ACHKUN_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO6661324.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10542..10874)
                     /gene="cusF"
                     /locus_tag="ACHKUN_003109"
     CDS             complement(10542..10874)
                     /gene="cusF"
                     /locus_tag="ACHKUN_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO6661325.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11032..12405)
                     /gene="cusC"
                     /locus_tag="ACHKUN_003110"
     CDS             complement(11032..12405)
                     /gene="cusC"
                     /locus_tag="ACHKUN_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO6661326.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            12562..13245
                     /gene="cusR"
                     /locus_tag="ACHKUN_003111"
     CDS             12562..13245
                     /gene="cusR"
                     /locus_tag="ACHKUN_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO6661327.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13235..14683
                     /gene="cusS"
                     /locus_tag="ACHKUN_003112"
     CDS             13235..14683
                     /gene="cusS"
                     /locus_tag="ACHKUN_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO6661328.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3535 a         3983 c         3660 g         3544 t
ORIGIN      
        1 ctgacaaccg gatatgcggg gattgctccc cgcacaacgg ctacttcttc ggttcgtaag
       61 cgagaacagc cttaaactca atattacgtt cctttacggt gaactcacac agcgaatccc
      121 cgaccagaag cgtaaatccc agcaccgtta aacacagaac tatgactgcc gcaagggcat
      181 atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc caaattgttc
      241 aggtttgggt aggtagcccc agggttgata gaaatatcgt cctgggactt catctttcca
      301 gacctcagag taaacctgaa accggaaagc ttcaggcacc cgcagttatc ttacccttgc
      361 ttagcaaaat agctgtttat ttatacagta agtcctgttt cggacttctc actccccatt
      421 tgatcaactg aagctgaacc cgtttttttc tgcatgcctc aatcgtcaaa attcttcgcc
      481 accagccagc cgtgccataa cttgtcacat tgcaaaagta gccaaattgc ttcagtcacc
      541 atcaccaaaa aaagaaacat tccagaactt ccgcatctcg cccagccgct ccgacttcta
      601 tactgaatag aaaacgccaa cttaagagaa acctatgcca ttacccgatt ttcatgtttc
      661 tgaacctttt accctcggta ttgaactgga aatgcaggtg gttaatccgc cgggctatga
      721 cttaagccag gactcttcaa tgctgattga cgcggttaaa aatgagatca cggccggaga
      781 ggtaaagcac gatatcaccg aaagtatgct ggagctggcg acggatgttt gccgtgatat
      841 caaccaggct gccgggcagt tttcagcgat gcagaaagtc gtattgcagg cagccgcaga
      901 ccatcatctg gaaatttgcg gcggtggcac gcacccgttt cagaaatggc agcgtcagga
      961 ggtatgcgac aacgaacgct atcaacgaac gctggaaaac tttggctatc tcatccagca
     1021 ggcgaccgtt tttggtcagc atgtccatgt tggctgcgcc agtggcgatg acgccattta
     1081 tttgctgcac ggcctgtcac ggtttgtgcc gcacttcatc gccctttccg ccgcgtcgcc
     1141 gtatatgcag ggaacggata cgcgttttgc ctcctcacga ccgaatattt tttccgcctt
     1201 tcccgataat ggcccgatgc cgtgggtcag taactggcaa caatttgaag ccctgtttcg
     1261 ctgcctgagt tacaccacga tgatcgacag cattaaagat ctgcactggg atattcgccc
     1321 cagtcctcat tttggcacgg tggaggttcg ggtgatggat accccgttaa cccttagcca
     1381 cgcggtaaat atggcgggat taattcaggc caccgcccac tggttactga cggaacgccc
     1441 gtttaaacat caggagaaag attacctgct gtataaattc aaccgtttcc aggcctgccg
     1501 ctatgggctg gaaggcgtca ttaccgatcc gcacactggc gatcgtcgac cgctaacgga
     1561 agataccttg cgattgctgg aaaaaatcgc cccttccgca cataaaatgg gtgcatcgag
     1621 cgcgattgag gccctgcatc gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg
     1681 cgatttcgtc gccgatggtg gctcgctgat tgggctggtg aaaaagcatt gtgagatctg
     1741 ggccggtgac taagtaggga attgccttac agaagaatat ccctgacaat ggtgttttaa
     1801 cttacattta acaatgttat gaaacaccct ttagaaacct tgactaccgc agcaggcatt
     1861 ttgctgatgg ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg
     1921 gcacaaaaac tggtgaccat gttccatctg atggatctta gccagcttta cactttattg
     1981 ttttgtctgt ggtttttagt gctgggcgct attgagtatt ttgttctgcg ctttatctgg
     2041 caacgctggt tctcgctggc ggattaaacg tcgattatcg ccgtacttcg cggcatactt
     2101 tgcttattct ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc
     2161 gaaacgccta gcccttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt
     2221 cgatgttttt aaaattggcg gcaaaatttt tatgctgtcg tcggagctgc gcggcgtccc
     2281 ctttatcaat ctgaagtccg atccacaaaa atccctgtta aatcagcaga tatacccgag
     2341 cattaagcca gggtatcaca tgaataaaaa gcactggatt tcggtgtatc ccggcgagga
     2401 aatctccgaa gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg
     2461 tctggctaaa cgcgatcaaa aaagagtgcg tccaggctaa agcggaaatc tatagcgcat
     2521 ttttctcgct taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattactttc
     2581 acatggagtc tttatggata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt
     2641 tgatgccagt aaaaaactta ccccggaaca ggccgagcag atcaaaactc tcctgcaata
     2701 cagcccatcc agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg
     2761 taaagcgcgt gttgccaaat ccgctgccgg taattatgtg ttcaacgaac gtaaaatact
     2821 tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa
     2881 gctggttgtt gaccaggaag atgctgatgg ccgctttgcc acgccggaag cgaaagccgc
     2941 gaacgataaa ggtcgcaagt tcttcgctga tatgcaccgt aaagatctgc atgatgatgc
     3001 agagtggatg gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc
     3061 gctgggtctg gacgcggtac ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt
     3121 tggtctgaaa gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt
     3181 tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg caaaacatca ccttaaccga
     3241 agtgtaattc tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt
     3301 gcataaccct gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag
     3361 gatttaatat cccaggttga agatttagcg ggtattgaga tcgatcacac cacctcgatg
     3421 gtgatgattt tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg
     3481 gtggtactgc ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc
     3541 attacccaga ataaactctt ccaccgttta gcttttaccc tgcaggggat tatcgtcaat
     3601 attcaggcgg tattctggct gcaaaaaggc accgaagcgg cagatattct gactacctgc
     3661 gcgcagttgt ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgttatt
     3721 ttgaatctgg cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag
     3781 gggattaaac tgatcggcgc aatcctggtc ggcattttga tgatctcgct gctgattggt
     3841 cagtcgccag cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggtt
     3901 tttaaagatc cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg
     3961 aaactgggcg actggctgga gatgccgaaa tacggcgcgg atggcgcggt gatcgatatt
     4021 gggttaacca ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg
     4081 tctctggttt ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt
     4141 attaagcgca gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg
     4201 caacgtctga ataaagcgca cttgttaaaa ccttatttaa ccagccgcca tcaggaaatt
     4261 aatgagtgga atcgccagca aggttctacg gagtcgatat taaacctgcg ccgaatgacc
     4321 aatattggaa cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt
     4381 aaagatatga ccttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag
     4441 atctacgcgt ttactaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata
     4501 ttcgatcaca tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccaacc
     4561 ggcaatgata ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca
     4621 tcgaatggat gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac
     4681 gcagcgtttt aaatgccaca aacaggaata caatccacac cggcagcagg atcgctgaca
     4741 agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcaatgcaaa
     4801 gatagttgcc gaacggatag agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca
     4861 tcgctgcacg aaaacgcaga tgcgccagac agatcataat ccagttcaac agcagcgttg
     4921 ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaata
     4981 ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc
     5041 tgacgcgagt caaaaacttt ggcgcattac cctgcacaga aaggccaaac agcatgcggc
     5101 tgttagagta aaccccgctg ttatacactg acagcgatgc taccagaatg acgaagttca
     5161 gcgcagaagc taccacgttg ctgtcgagat tatggaaaat catcacaaac gggctactgt
     5221 tggatttcac ttccacccac ggatagagcg ccagtaaaac caccagtgaa ccgatgtaaa
     5281 acagcaggat gcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc
     5341 gcgcttcagc ggcagtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg
     5401 ccagcgacaa aatcagccca ttccagccgg tggcaaagaa accaccgtag cgccagaggt
     5461 tgtcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg ccaaagccga
     5521 tcataccgat gatcgccagc actttaatca gcgcaaacca gaactcggtt tcaccatata
     5581 agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc
     5641 acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca
     5701 ttcccaccag cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc
     5761 cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcttcg accaccatct
     5821 cgccaagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata
     5881 cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg
     5941 cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct
     6001 cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg
     6061 tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc
     6121 cacacggtta aacggggtat cctgctttta tttccgtacc cgatgtcggt gcagccacat
     6181 cagcttatac gccgccggga taataaacag cgacagcaaa ggtgcggtga tcatgccgcc
     6241 aatcatcggt gcggcaatcc ggctcatcac ctctgaacca gcccccgttc cccacagaat
     6301 cggcagcaga ccggcgataa tcaccgccac cgtcatcgct ttcgggcgca cgcgcaggac
     6361 cgcgccgtga tataacgcct catccagctt ctgctcgctg aatgtttgtg gattattcaa
     6421 cgacggctcg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc caaattcggc
     6481 ggcgaccccg gcgagggcga taaagccagt gcccgtcgcc acggaaagat gaaagcccat
     6541 ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga taatcagcaa
     6601 cgcttcgccc acccgacgga acgccagata caacagcacg aagataatca tcaacgtcat
     6661 cggcaccatg agtttaagct tatggttggc gcgctccagt agctcgaact gcccggagaa
     6721 tgccacgctg gtgcccggtt ttaactgcac tttttcagct atcgcttttt gcaaatcgtg
     6781 aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg tcgggcgcgc
     6841 attctcggtt ttcagcattg acggtccggt agagacttta atgtcggcca cgtctgccag
     6901 ggtgatttgc tgcttcatcg gcgtcaggat cggcaactgg cgcagtgcct gcggactatc
     6961 gcgccagctt tgcggataac gcagattaat tggataacgg gcaatccctt ccaccgtttc
     7021 gccaaccatc gccccgccca ccgcagaagt cacaaacaac tgcacatccg ccaccgtcat
     7081 accgtaacgt gcggcttttt cacggttaat ctcaacgttg atatagcgcc caccttccag
     7141 acgctcggct agagcagaag ccacgcctgg caccgttcgc gctacttctt caatttgctc
     7201 agccatcgcg tcgatatccg ccagcacagt gccggaaact ttaatgccga tggggctttt
     7261 aatgccggtt gagagcatat cgatacggtt acgaattggc ggcacccaca gattcgccag
     7321 ccccggcaga cgcacggtgt tatccagttc ctcaatgatt ttgtccattg tcatgcctgg
     7381 ccgccactgc tcctgcggct taagctggat ggtcgtttct accatctcca gcggagcaga
     7441 atcggtggcg gtttccgctt tcccggtttt gccaaatacc cgcgccactt caggtacgct
     7501 cataattagc ttgtcggttt tttgcagcat actcgccgcc tctgctgcgg aaatccccgg
     7561 cagcgtcgat ggcatataca acaggtcgcc ttcattgatc tgcggtaaaa attccccgcc
     7621 aactttattg agcggccaga gaaccgtcag caccgaaagc gctgccacca acagcgtggt
     7681 tttcggccag tgcagtactt tcagcaacag cggatgataa acacgaatca aaaagcgatt
     7741 gagcgggtta ctgctttccg gcggaatttt gccacggatc cagtaaccca tcaggatagg
     7801 gatcactacg atcgccagca gcgccgcacc cgccatcgca tacgttttgg tgaacgccag
     7861 cgggccaaac agacgccctt cctgcccttc cagggtgaag atcgggataa acgacaacgt
     7921 gataatcagc agactgataa atagcgccgg acccacttca acagatgcat cggtgatcac
     7981 ctgccagcgc gttttattat ccagcgtggc gtcaggatgc tggtgctgcc actcttccag
     8041 ccgtttatgt gcattttcga tcatgacgat agcggcatcg accatcgccc cgacggcaat
     8101 cgcaatgccg cccaacgaca taatattggc attcagtccc tggaagtgca tgacaataaa
     8161 agcaatacac aaccccagcg gcaacgaaat aatcgccacc agcgccgagc gcacatgcca
     8221 gagaaacagc gcacagacta ccgccaccac aataaactct tccagcaact tgccgctgag
     8281 gttgtcgatg gcgcggtcaa tgagctggct gcgatcgtat gttgtaacta tctccacgcc
     8341 ttccggcaga ctacttttca gcgtttccag tttgtccttc acggcggcga tcacttctcg
     8401 ggcgtttttg ccggatcgca ggatcaccac cccgcccgcc acttcgcctt cgccgtttag
     8461 ttcggcaatg ccccggcgca tctccgggcc aatctggacc ttcgcaacat cgcgcagata
     8521 aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg
     8581 cagatagccg ctggcgcgca ccatatattc cgcttccgcc agttcgatcg acgaaccgcc
     8641 cgcttcctgg tttgaagcat ccagcgcgct ttttacttcg gcgagactga tgccatactg
     8701 cgccaggcgc tggggatcga taaccacctg atactctttc accacaccgc ccaccgacgc
     8761 cacttccgca acgtcaggga tggttttcag ctcatatttg agaaaccagt cctgcaatga
     8821 gcgtaaatcg gccagatcgt gcttaccgct gcgatccacc agtgcatatt catagatcca
     8881 gccaacaccc gtggcatctg gtcccagctc ggcgctgact cccgcaggca gcttaccctg
     8941 tacctggttg aggtactcca gcacccgcga acgcgcccag tacggatcgg tgccatcttc
     9001 gaaaatgaca tacacataag agtcgccaaa ctgtgagaaa ccgcgcacag tcttcgcgcc
     9061 aggcaccgac aacatggtgg tggttagcgg ataagtcacc tgattttcaa cgatttgtgg
     9121 tgcctgaccg ggatagctgg ttttaataat aacctgcaca tcggagagat ccggcagcgc
     9181 atccactggc gtattaatga tggtccaggt gccccagatg ctcagaaaca acgcgcccat
     9241 cagcaccaaa aaacggttcg ccaccgagcg acgaataatc cattcaatca ttggttattc
     9301 cctcaatgcg catgggtagc actttcagag cgcatccgct ccagtgcgcc agaaatattg
     9361 gcttcggaat caatcaggaa caggccgctg gaaaccacct tttcaccttc cgccagacca
     9421 gagcgtaatg cggtgacgcc ttgcgatgcc tggaaaacag caacgcgttt cggtacaaag
     9481 cgcccgtcgg catcaacggt aatcacccgc tgttcgctgc cggtatcaat cagcgcttgt
     9541 gacggaatga gcagcatcgg ttcgctggcg gtgttgagtt gcaaccaggc gttcatgccc
     9601 ggttttagcg cctcgtcggc gttgtcgact tccagacgca gctgcagcgt gcgggtcgcg
     9661 gcatccacgc caggtagcag cgtccatttg cggatggtga gtgttttatc cggtcgcgcc
     9721 ggaacggtga gggtaaactg cgaggcatct ttcaccagcc aggcgatgga ctccgggatc
     9781 gcagcagtga cccacaccgg gtccataccc tgaattttcg cgaccacgtt atctttggcg
     9841 atattcattc ccgcgcgcag atcaaacgcg gtgatcacgc catcaatggg cgctttgagc
     9901 gtaaagcgag tctggatttt ttgcgtggcg atcaggcggc gaatatccgc ctccggcatt
     9961 cccgccagtc gcagccgctc aagaatgcct tcagtctggg tcgccgtacc gccggtttcg
    10021 cgcagcagta aatactcact ctgcgcttcc acccagtcag gaatggtcag gtcgagaagc
    10081 ggtgtgccct tttgtacttt atcgcccacg gtaagcggat acaccttgtc gataaacccg
    10141 gcagcgcgag cctgcacaat ggcatactga tactcgttgt aactgacatt cgccgggaaa
    10201 ctctgggcaa aagtcagcgg cccgcgcgtg acggtagccg ttttcacccc cagattctgc
    10261 gtctgagtcg ggtcaatgcg cacaccagac gcagaactct cttcatcggc atatttcggc
    10321 accagatcca tatccataaa cggcgattta cctggtttat cgaaccgcgt attggggtac
    10381 atcgggtcgt accagaataa gattttacgt tctgcggtcg acgttttttc tgcgggtggt
    10441 tcagcctttg caaaccaggt aaaacctgcc gcagaaataa taccgcccgc gatcatgctg
    10501 ccgataataa gcgcgatttt tttcatctca ttaaacctgg gttactggct gactttaata
    10561 tcctgtaata aagaaaggtt gccctgctgg acaaaattaa acgccacttt gtcgccagtt
    10621 ttaatttcac tcattttcgt ctgcggagtg atggtaaagc gcatggtcat ctccggccag
    10681 ttcacggcag caatcggatc gtgatggatg gtgatttttt tgctttccag atcaataccc
    10741 tttaccacgc cagtggcgct aataacctgc ggttgtactt cgctcatggt ttcatgatga
    10801 tgttcgttag cctgggcatt aaagccaata acggtaaaca gactgaacat tgcgacttgc
    10861 agtgcttttt tcatttattc tctcctggag ttaaaagtca tactctaaat aattcgagtt
    10921 gcaggaaggc gacaagcgag tgaatcccca ggagcttaca taagtaagtg actggggtga
    10981 gcgaacgcag acgcagcaca tgcaacttga agtatgacga gtatattgct gtcaaccgcc
    11041 acctaaggcg gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa
    11101 agtttgtcgg gttgcaaata aagaacgctc ggcatccagc acttccagat aacttactgc
    11161 gccgtgctga tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg
    11221 ctgctgggcg ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc
    11281 tttaaaggcg ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc
    11341 ggcgatatcc agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg
    11401 aataaaattc cacatcccgc tgctggcgtt aaataatgac gacagatcgc tgctggcggt
    11461 tgatattccg ctggtcagcc ttatagatgg gaaaaatgcc gcgcgtgcgg ccccaatatt
    11521 ggcattagcc gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat
    11581 ttgcgacgac aatcccgccg gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg
    11641 cgcttgcggc agcttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc
    11701 cagttccccc tgacgtttag cgatgtcgct gcgggtactt tcaatcaccc cgcgagcctg
    11761 ttccagcgcc agaacattac tgctacctgt caacagttgt ttttcgacaa acgcatatga
    11821 ctgctgataa ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ctagctgctg
    11881 attgaaataa ctttgcgcga cattagaaac cagcaaaata tgcaccgcgc gctgggcttc
    11941 ctcagtggct aaataatttt ggcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa
    12001 atcgagatca aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc
    12061 tttaagatta ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc
    12121 atcggtcaga cgatattgcg cccgcgtttc ctgcactttc agcgtcgcca tgcgcaaatc
    12181 ccggttattc accagcgcct cgctaatcag cgtctttacc tgattatcaa caaaaaaggt
    12241 gcgccagccc acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga
    12301 gaactgctgc ggcacgggca ttgccggacg ctgataatcc ggtgccagtg aacaaccggt
    12361 tagcgcaagg gccacacaaa atggcagaag tttacaagga gacataggct cataatttct
    12421 ggtgatttta taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg
    12481 acaaaattgt cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc
    12541 cgggcaacat gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga
    12601 gaatacttga ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg
    12661 ctgaatggct accatctggc gatgaccggt gattatgatc tgataatcct cgatattatg
    12721 ctgccggacg tgaacggctg ggatatcgtg cgcatgttgc gttccgccaa taaagggatg
    12781 ccgattctgt tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg
    12841 ggggcagatg actacctggt gaaaccattc gcttttgctg aactgctggc gcgggtgcgc
    12901 actctcctgc ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg
    12961 atggtcgatc tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt
    13021 aaagagttta ctctgctgga gtttttcctc cgccatcagg gcgaagtgct gccccgctcg
    13081 cttatcgcct cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgta
    13141 gcggtgaagc ggctgcgcgg caaaatcgac aacgactttg agccaaagct aattcagacc
    13201 gtgcgcggcg tgggttacat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc
    13261 gttttcgctg gcaacccgcc tgaccttttt tatcagcctg gccaccatcg cagcgttttt
    13321 cgcctttgca tggatcatga tccactcagt aaaagtgcat tttgccgagc aggatattaa
    13381 tgatttaaaa gaaattagcg ccacgcttga acgggtactt aatcatcctg acgagacgca
    13441 agcccgacgc ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatctc
    13501 cctggcagat agccacggca aaacggtgta tcactccccc ggtgcgcctg atatccgcga
    13561 gtttgcacgt gatgccatac ccgataaaga cgcgcggggt ggcgaggtgt atctcctttc
    13621 cggcccgacg attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg
    13681 gatgattaac ttgtcggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat
    13741 cgcgctttcg atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat
    13801 gaccgcatcg gtaatcagca tcctgatcgt ctttatcgta ctgttggcgg tacataaagg
    13861 tcacgcgccg atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga
    13921 cgttcgcctt gacccgcaga ccgtgcccat tgagctggaa cagctggtac tgtcgttcaa
    13981 ccatatgatc gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat
    14041 cgctcacgaa attcgcacgc cgattaccaa tctcatcacg caaacggaaa tcgccctcag
    14101 ccagtctcgc agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac
    14161 gcgaatggcg aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct
    14221 aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt
    14281 cgaggcgtta gcggaagatc gcggcgtgga gttgcgattt gttggcgacg aatgtcaggt
    14341 tgcgggcgat ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg
    14401 ttatacgccg cccggagagg caattgtagt gcgctgccag acggtcgatc accaggtgca
    14461 agttaccgtc gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga
    14521 ccgtttctac cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct
    14581 ggcgatagtg aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc
    14641 gcgggggaca aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc
    14701 agggcgtggt gcatcacctc ct
//
LOCUS       DAZFRJ010000115        14445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000115 DAZFRJ010000000
VERSION     DAZFRJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.116"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..386
                     /locus_tag="ACHKUN_003113"
     CDS             30..386
                     /locus_tag="ACHKUN_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6661329.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(437..1333)
                     /gene="yhaJ"
                     /locus_tag="ACHKUN_003114"
     CDS             complement(437..1333)
                     /gene="yhaJ"
                     /locus_tag="ACHKUN_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6661330.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1438..2139
                     /locus_tag="ACHKUN_003115"
     CDS             1438..2139
                     /locus_tag="ACHKUN_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO6661331.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2162..2326
                     /gene="yhaL"
                     /locus_tag="ACHKUN_003116"
     CDS             2162..2326
                     /gene="yhaL"
                     /locus_tag="ACHKUN_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO6661332.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2538..3848)
                     /gene="cyuA"
                     /locus_tag="ACHKUN_003117"
     CDS             complement(2538..3848)
                     /gene="cyuA"
                     /locus_tag="ACHKUN_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO6661333.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3876..5207)
                     /locus_tag="ACHKUN_003118"
     CDS             complement(3876..5207)
                     /locus_tag="ACHKUN_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6661334.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5482..6846)
                     /gene="tdcG"
                     /locus_tag="ACHKUN_003119"
     CDS             complement(5482..6846)
                     /gene="tdcG"
                     /locus_tag="ACHKUN_003119"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6661335.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6918..7307)
                     /locus_tag="ACHKUN_003120"
     CDS             complement(6918..7307)
                     /locus_tag="ACHKUN_003120"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO6661336.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7321..9615)
                     /gene="tdcE"
                     /locus_tag="ACHKUN_003121"
     CDS             complement(7321..9615)
                     /gene="tdcE"
                     /locus_tag="ACHKUN_003121"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO6661337.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9649..10857)
                     /gene="tdcD"
                     /locus_tag="ACHKUN_003122"
     CDS             complement(9649..10857)
                     /gene="tdcD"
                     /locus_tag="ACHKUN_003122"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO6661338.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGSSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10883..12214)
                     /gene="tdcC"
                     /locus_tag="ACHKUN_003123"
     CDS             complement(10883..12214)
                     /gene="tdcC"
                     /locus_tag="ACHKUN_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO6661339.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12236..13225)
                     /gene="tdcB"
                     /locus_tag="ACHKUN_003124"
     CDS             complement(12236..13225)
                     /gene="tdcB"
                     /locus_tag="ACHKUN_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO6661340.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13324..14262)
                     /gene="tdcA"
                     /locus_tag="ACHKUN_003125"
     CDS             complement(13324..14262)
                     /gene="tdcA"
                     /locus_tag="ACHKUN_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO6661341.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3747 a         3874 c         3369 g         3455 t
ORIGIN      
        1 tcgtcacttt taattaaagg gatgttttta tgcagtggta tttagccgta ttaaaaaatt
       61 atgttggttt ctctggtcgc gcacgtcgta aagagtactg gatgtttact ctgattaacg
      121 ccatcgtcgg cgctattatc aatgtcattc aattgatttt aggtttagag tttccattcc
      181 tttctctcat ttacctggca gcaacaatca ttccggtcat tgctctgtgt gttcgtcgtc
      241 tgcacgatac tgaccgttca ggtgcgtggg cgcttctcta tctggtaccg atcattggct
      301 ggttagttct gtttgtattt gcctgtttag aaggcaattc tggcagcaat cgttacggaa
      361 acgatcctaa gtttggttca aattaatctt agaattgggg cgacatttcg ccccttttta
      421 ttaataataa caaatattat tttccgctaa aaagtttggg aatttcccgc agacaccagg
      481 atttcgcttc ccccatactg tcacgacgcc aggccataat aatatcgatc tcgctggtcg
      541 attccgggct gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg
      601 gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca
      661 ccgttaagcg cggctgtttg tccagcagct gtacggtcaa caccgggcgc tcacgagcgg
      721 tatccgctac cgcaattcca cgatatttca cgcgcgtcac ttcagataac ggttccggct
      781 cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc
      841 gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataaca atatccgccc
      901 gcccctgctc cagtcgttcc cacgcccccg ccagcacttc ggtgatgatt gccagttggg
      961 tattggcttt tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg
     1021 cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagggcttcc gcatcggtag
     1081 tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat
     1141 tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca
     1201 gtttttgcat ggtgtagcta agtgcagaag gcacgcgtcc cagctcatcc gccgccgccg
     1261 caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc
     1321 tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc
     1381 taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg
     1441 attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt gcaggcccgg
     1501 tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc ctccctgcgt
     1561 gtgcttaacc aggaagtgct ggccccagat gccgcctttc agccgcgaac ctatcccaaa
     1621 gtcgatattt taaatgtgat tctggatgga gaagcagagt atcgcgacag cgaaggcaat
     1681 catgttcagg ccagcgccgg tgaggcgttg ctgctctcta cccagccggg tgtcagctac
     1741 agcgaacaca atctcagcaa agacaaaccg ttaacacgaa tgcagctttg gctggacgcc
     1801 tgcccgcagc gagagaatcc gctgattcaa aaactggcgc ttaatatggg caagcagcaa
     1861 ttaatcgcct cgccagaggg tacgatggga agcctgcaat tacgccagca agtgtggctg
     1921 caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg
     1981 tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg
     2041 ctgacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat
     2101 tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat
     2161 gatgagtaaa aaatcggcca aaaagcgcca gccagtgaag cccgtggtgg cgaaagaacc
     2221 tgctcgcacc gccaaaaatt ttggctatga agagatgttg agcgagctgg aagccatcgt
     2281 cgcggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg
     2341 atgcggcgaa aacgccttat ccggcctaca aaatccaaca acattaacga attaaacaac
     2401 ccgtaggccg gataagatgc gccagcatcg catccggcat caggtacaaa acacaacaac
     2461 atcaacaagt tgagcacccc gtaggccgga taaggtgcgc cagcatcgca tccggcacga
     2521 tacccaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat ctgccgatcc
     2581 gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg ctcaacatca
     2641 tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat taacaccgct
     2701 ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg
     2761 caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat ggtttcataa
     2821 cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt cgttgcggca
     2881 cacagcgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa atgagaaagc
     2941 atcagcgcac gcgccagccg ttcatcatcc gctccgaagt gttctgctac caccaccaca
     3001 ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcatagc cggaagcgta
     3061 gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga
     3121 tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc
     3181 cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga atcgagaata
     3241 aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc cagcgtcgtt
     3301 ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc ctgctgggta
     3361 aacaccacac cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg
     3421 acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat ttcattgcaa
     3481 ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc atcggcaatt
     3541 gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc acctaacgcc
     3601 cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt gacgcccaga
     3661 ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg accttccagt
     3721 tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc
     3781 ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt taaagtcgaa
     3841 tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag gaacggagaa
     3901 acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt gtatttgtgc
     3961 aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat gccgaaaatc
     4021 gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc gctccaggcc
     4081 agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat cttctcggca
     4141 ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa
     4201 tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct gaccactttc
     4261 acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc
     4321 tgctcatagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag
     4381 aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa tgctttatgc
     4441 cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc
     4501 tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc
     4561 agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac catcgacacg
     4621 cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat tttgaataac
     4681 aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag accataaaag
     4741 ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga agcactatcg
     4801 ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa atagagcgca
     4861 cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg gtaatcttta
     4921 cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata cattgccggg
     4981 taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac ctgcaccggg
     5041 agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca gccggtgtca
     5101 gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc acgacccgcc
     5161 ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat ttctcgctca
     5221 atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg cggtctacgt
     5281 ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag aaaatcttca
     5341 caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt aatcgcaaga
     5401 gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt gtgcacccaa
     5461 ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc ctccgcgtga
     5521 tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct cgatcacttt
     5581 atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc
     5641 tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg
     5701 atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc atacctgcgc
     5761 cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg ccacgccaat
     5821 ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat acagcgcgcc
     5881 aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg gacgacggaa
     5941 cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag tcggtgccgt
     6001 taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca tgttgatcca
     6061 gtcgatgaca ttcatcggat cgttagagat gttatcgctg gaaaccagct gacgacgcag
     6121 cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag tgttcatccc
     6181 gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt caatttccgc
     6241 tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca ggccgttgta
     6301 atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg acgtttcgac
     6361 atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga cagagtaata
     6421 ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc cgttctcatg
     6481 gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat caacaatatg
     6541 cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa atgcagggat
     6601 ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa tggcgacatc
     6661 cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca caatatggct
     6721 cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc cggcattcat
     6781 tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat cgaatgcact
     6841 aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga ccttgcgacc
     6901 gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc cagcttcaca
     6961 tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca
     7021 tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc ggtgatgaac
     7081 acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat cgctttgacg
     7141 ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc ggtctgcggg
     7201 caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc ctgaacataa
     7261 gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca tcctccggcg
     7321 tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc
     7381 gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt tttccgggtg
     7441 ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt gttgaccgcc
     7501 ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt tggttttacg
     7561 tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg agatcccatc
     7621 tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg cacctttgcg
     7681 gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc
     7741 cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca gaatcgactg
     7801 ggtagtgacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa agcgttcaac
     7861 caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc
     7921 gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca cgcgggcata
     7981 tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc atgccatagt
     8041 gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg
     8101 catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt ccatgaagtg
     8161 atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt
     8221 cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt agagcagcgt
     8281 tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca ccattgggct
     8341 gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt
     8401 ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt tgaaggcaat
     8461 cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg
     8521 cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt ccagccccat
     8581 cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat caaattccgg
     8641 tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca gttcctgtgc
     8701 ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca atgtagatat cgaggaacga
     8761 tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg ccagataagc
     8821 gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga tatcaaagcc
     8881 atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg ccagctcctc
     8941 acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact ggagatcggc
     9001 aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta cgcggcgata
     9061 gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca cgccagattt
     9121 acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat gggttttacg
     9181 cagatcggta aacagatatt caaattcact gtccatttca cggccatagg cgtggaatga
     9241 acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt
     9301 ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat gagcggtaat
     9361 tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat
     9421 accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg cgaggaaaga
     9481 ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat taatttcgtt
     9541 tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat cgctggtatc
     9601 aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt atgcaaattc
     9661 tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt
     9721 agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc gctcaccaca
     9781 ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag ccaaatgttc
     9841 catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga atataattcc
     9901 atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc ggtgaacaaa
     9961 ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt ccagaacacg
    10021 taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca ctacgcgttc
    10081 caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac caaagtcgac
    10141 atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca ttgaagtatc
    10201 aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat gcgccacaac
    10261 caggccggag tcatcttccg ccagattcag cagcgaatgt gcgcgctggg aaacatagcg
    10321 gtgcgacgag ccgtggaaac cataacggcg tacacctaac tcttcataat atttccacgg
    10381 caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat caaataccgc
    10441 cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta aattggcgta
    10501 attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt catcggtaat
    10561 aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa ttaaggccac
    10621 actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg caccttcgta
    10681 gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg cattttccga
    10741 gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat cgagtacgga
    10801 aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt cattcatctc
    10861 ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg atggtcagca
    10921 gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc agagacggcg
    10981 ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc ggtgcgccca
    11041 tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag gtggagccca
    11101 tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt ttgtcgcctt
    11161 tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga tagtgaccga
    11221 agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc agtgtaatcg
    11281 cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa gaaagcactg
    11341 gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac aggcagctaa
    11401 aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg atttgggaac
    11461 atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt ttagaaacca
    11521 cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa atccccagcc
    11581 agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt
    11641 cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc aggctggcga
    11701 taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca aaccagatga
    11761 cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc
    11821 cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca taaatccaca
    11881 gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca gttttaccaa
    11941 aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa agacacagac
    12001 gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac atcagaagaa
    12061 tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg gcgccgattg
    12121 ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac gattgttttg
    12181 tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga attaattaag
    12241 cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg ccggaaataa
    12301 tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat aatgcagcac
    12361 atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag gcaatcatac
    12421 tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca cgaacgattt
    12481 cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg ccggtagttc
    12541 ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg ttttcagact
    12601 gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca ataccagcaa
    12661 ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga tcttccataa
    12721 tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa ggtgggataa
    12781 aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg ttgaaattat
    12841 caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg gattttggcg
    12901 caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc
    12961 cttgcgcatg gttgcccgca gaacaggcca ctacaccttt acgtttttcc gcatcagtca
    13021 gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc tgcatatttt
    13081 caaacttcag gaatatttca cctttgcaac gttcactaaa atagtttgag cgaggcatgc
    13141 ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatgtca tcaatagcaa
    13201 ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa atatgtaggt
    13261 aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa taaaaacaaa
    13321 taactaacca acttctatta attgccttcg tctacaccca ttataagatg aatactcttt
    13381 ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg accataccac
    13441 ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt gatttgaacc
    13501 aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt
    13561 ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc catttctttg
    13621 taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca cccactgttc
    13681 gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccag
    13741 cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcact
    13801 taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag
    13861 ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat
    13921 ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc
    13981 ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg taatggattc
    14041 ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac
    14101 cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga cggccggttg
    14161 agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga cttcctgaaa
    14221 gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta
    14281 cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac
    14341 aaattaactt tcaatacttt ccatggtatc gttacgcaaa ttaaatacct ttaaatatca
    14401 acaagttaaa gtataaaaat cagcataaac cctgattttt gtcaa
//
LOCUS       DAZFRJ010000116        14408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000116 DAZFRJ010000000
VERSION     DAZFRJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.117"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..582
                     /locus_tag="ACHKUN_003126"
     CDS             16..582
                     /locus_tag="ACHKUN_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6661342.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            633..3092
                     /locus_tag="ACHKUN_003127"
     CDS             633..3092
                     /locus_tag="ACHKUN_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6661343.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3107..3838
                     /locus_tag="ACHKUN_003128"
     CDS             3107..3838
                     /locus_tag="ACHKUN_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6661344.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3835..4896
                     /locus_tag="ACHKUN_003129"
     CDS             3835..4896
                     /locus_tag="ACHKUN_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6661345.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5048..6094
                     /locus_tag="ACHKUN_003130"
     CDS             5048..6094
                     /locus_tag="ACHKUN_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO6661346.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHKUN_003131"
     CDS             complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHKUN_003131"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO6661347.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHKUN_003132"
     CDS             complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHKUN_003132"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO6661348.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHKUN_003133"
     CDS             complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHKUN_003133"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO6661349.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHKUN_003134"
     CDS             complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHKUN_003134"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO6661350.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHKUN_003135"
     CDS             complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHKUN_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO6661351.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHKUN_003136"
     CDS             10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHKUN_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO6661352.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHKUN_003137"
     CDS             complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHKUN_003137"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO6661353.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13199..13708)
                     /locus_tag="ACHKUN_003138"
     CDS             complement(13199..13708)
                     /locus_tag="ACHKUN_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO6661354.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13859..14408)
                     /locus_tag="ACHKUN_003139"
                     /pseudo
     CDS             complement(13859..14408)
                     /locus_tag="ACHKUN_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3468 a         3851 c         3505 g         3584 t
ORIGIN      
        1 ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
       61 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
      121 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
      181 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
      241 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
      301 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
      361 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
      421 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
      481 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
      541 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
      601 tatcgccgcc tttcataaaa tatttatcag gtatggacac agtgaatatt tatcgactct
      661 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta
      721 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      781 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
      841 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
      901 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
      961 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1021 taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
     1081 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
     1141 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
     1201 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat agcgatgata
     1261 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
     1321 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
     1381 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
     1441 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1501 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
     1561 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1621 aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
     1681 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1741 atgatgtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg
     1801 gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg cttggcgcgc
     1861 tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac gagcggggct
     1921 taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg atttccctcg
     1981 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt gatcacaaat
     2041 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
     2101 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2161 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
     2221 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
     2281 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2341 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2401 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga gcccaggtga
     2461 gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2521 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2581 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2641 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2701 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2761 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
     2821 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
     2881 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
     2941 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
     3001 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
     3061 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
     3121 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
     3181 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
     3241 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
     3301 cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacgca
     3361 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
     3421 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
     3481 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
     3541 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
     3601 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
     3661 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacatcgg
     3721 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
     3781 gacgacgctc aactacagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
     3841 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt
     3901 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
     3961 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt
     4021 aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat
     4081 gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac
     4141 aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt
     4201 gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac
     4261 aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct cgctcgcatg
     4321 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
     4381 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac tgcccaaaac
     4441 ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
     4501 tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt
     4561 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
     4621 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
     4681 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
     4741 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
     4801 aaaacctata cggctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
     4861 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
     4921 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
     4981 ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
     5041 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
     5101 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
     5161 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
     5221 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
     5281 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
     5341 gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc
     5401 tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
     5461 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac
     5521 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
     5581 agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg
     5641 catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     5701 gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg cgtatatggc
     5761 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
     5821 gctaccgcaa atcctgctgt cgatttttgc tctgttggct atttgtgcgg gtatggcgtg
     5881 ggggctgacc cggtttatgc atattgattt tatgactgcc tacctcgcca ccagccccgg
     5941 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
     6001 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg ggacctgcca ttgcacggtt
     6061 tatttcaacc tatgcgccga agcggtcggc ttagtgctgg cagccagggc accagtaaaa
     6121 cgggcgagat gacagcgtgg ttttctcaat gatgctgcca caacgttcgc acggttcgcc
     6181 atctcgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
     6241 cccccgcgta gcgtaagaaa atcgaggaat ctccagtaac gcgtgtgcga gtgcatccag
     6301 ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat
     6361 ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgctcgagca gtaatcctgc
     6421 aaactgacgg ttacgaaagc gcggcgacaa tacccgttct ttcaccacct ccggcgtcag
     6481 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
     6541 cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     6601 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     6661 gacgccgtaa agctgattat ggctgtagag cgttaattcg ttggaaaaat gagttaacaa
     6721 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
     6781 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     6841 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     6901 ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa tgtagctcgt
     6961 aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc
     7021 gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc
     7081 gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctggctgact tcgcggcacc
     7141 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
     7201 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat caatgccgga
     7261 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
     7321 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
     7381 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcaactgc
     7441 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aattgcaaca
     7501 ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat
     7561 ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     7621 tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta attgttcaaa
     7681 ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
     7741 aatcggctga ccgagcggaa tttgcgcaag atggtacata tcagcctcaa tgatgcaggc
     7801 aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg gctgcccgtt
     7861 atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc gatcggtggt
     7921 gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg agctaagctg
     7981 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     8041 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
     8101 atcacgcttc gctttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag
     8161 cccgccactc cccactttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
     8221 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
     8281 ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat ccagccgcgc
     8341 ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa cagttaactg
     8401 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagta ggttagcaat
     8461 gcgtaacgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     8521 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     8581 tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     8641 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     8701 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagtaag
     8761 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     8821 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     8881 tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca
     8941 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
     9001 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
     9061 accgtaatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
     9121 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
     9181 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
     9241 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
     9301 cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
     9361 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
     9421 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
     9481 ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
     9541 cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc
     9601 ccacggcacc aacggctcaa tttcgcccat gacagtgatc cccagtaacg ccgccagctg
     9661 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
     9721 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
     9781 tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc
     9841 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
     9901 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
     9961 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10021 ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac atattaagaa
    10081 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    10141 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    10201 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    10261 atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg
    10321 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    10381 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    10441 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    10501 ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg
    10561 tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt taaatccaat
    10621 gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    10681 tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac
    10741 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg tatgatcgcg
    10801 ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg cgttaataaa
    10861 aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct ggttctgctg
    10921 gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt atacgcctta
    10981 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    11041 cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc
    11101 tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg cttcgttaac
    11161 cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa tgccttcgca
    11221 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    11281 accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc
    11341 attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg
    11401 gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg
    11461 caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat ctacatgctg
    11521 ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct
    11581 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    11641 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    11701 gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag caccatacct
    11761 gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc
    11821 gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag catcacacct
    11881 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    11941 ggtaaccatc ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg ggttgtcggg
    12001 ttgaaaatac gaaaatacgg cgctgcatca gtacccgtag acgccgccca ctgccagcca
    12061 ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    12121 cgccagtcga tcaacaaatc tttcaccaga aaactggctg aaatcatccg tagccggtta
    12181 tgcatccagc cagtgctgtt aagctgacgc atggcggcat cgacaatcgg gtatcccgtt
    12241 ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac acgatccgtc
    12301 caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    12361 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    12421 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccagg
    12481 ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg ctcatattct
    12541 ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    12601 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgagggctct
    12661 atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc ttcccgcagc
    12721 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attaccggtc
    12781 ttcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    12841 agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg atagttataa
    12901 aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    12961 aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc tatttgtagc
    13021 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtcgcccac
    13081 tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgcg gcaggcggca
    13141 gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag atgggtagtc
    13201 ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc
    13261 ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa
    13321 gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc tggcgttctg
    13381 tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc
    13441 gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg ttgtaaaaat
    13501 catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg cggtactccg
    13561 gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc
    13621 gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata cggattaggg
    13681 tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca ggagtaagtg
    13741 tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca aatttacgaa
    13801 agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc cctttctatt
    13861 caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga cgggtttctt
    13921 ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta tttaattctt
    13981 tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct tttacagcga
    14041 ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg
    14101 catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttct gggatagctg
    14161 tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg tgcattgttg
    14221 agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa gagtgccgga
    14281 gcgtttttcc atcaattgca atgacgtctt tatcatttga tgaatggcaa tcacgcatcc
    14341 agttaataaa gcactcgtga aattttgcag gactgataca ggatacaact ctggcaatag
    14401 tatcgtga
//
LOCUS       DAZFRJ010000117        14354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000117 DAZFRJ010000000
VERSION     DAZFRJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.118"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            41..559
                     /gene="yfiR"
                     /locus_tag="ACHKUN_003140"
     CDS             41..559
                     /gene="yfiR"
                     /locus_tag="ACHKUN_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO6661355.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            549..1775
                     /gene="dgcN"
                     /locus_tag="ACHKUN_003141"
     CDS             549..1775
                     /gene="dgcN"
                     /locus_tag="ACHKUN_003141"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO6661356.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1791..2273
                     /gene="yfiB"
                     /locus_tag="ACHKUN_003142"
     CDS             1791..2273
                     /gene="yfiB"
                     /locus_tag="ACHKUN_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6661357.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2350..2697)
                     /gene="rplS"
                     /locus_tag="ACHKUN_003143"
     CDS             complement(2350..2697)
                     /gene="rplS"
                     /locus_tag="ACHKUN_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO6661358.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2739..3506)
                     /gene="trmD"
                     /locus_tag="ACHKUN_003144"
     CDS             complement(2739..3506)
                     /gene="trmD"
                     /locus_tag="ACHKUN_003144"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO6661359.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3537..4085)
                     /gene="rimM"
                     /locus_tag="ACHKUN_003145"
     CDS             complement(3537..4085)
                     /gene="rimM"
                     /locus_tag="ACHKUN_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO6661360.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4104..4352)
                     /gene="rpsP"
                     /locus_tag="ACHKUN_003146"
     CDS             complement(4104..4352)
                     /gene="rpsP"
                     /locus_tag="ACHKUN_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO6661361.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4601..5962)
                     /gene="ffh"
                     /locus_tag="ACHKUN_003147"
     CDS             complement(4601..5962)
                     /gene="ffh"
                     /locus_tag="ACHKUN_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO6661362.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6129..6920
                     /gene="ypjD"
                     /locus_tag="ACHKUN_003148"
     CDS             6129..6920
                     /gene="ypjD"
                     /locus_tag="ACHKUN_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO6661363.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6941..8227
                     /gene="yfjD"
                     /locus_tag="ACHKUN_003149"
     CDS             6941..8227
                     /gene="yfjD"
                     /locus_tag="ACHKUN_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO6661364.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8282..8875)
                     /gene="grpE"
                     /locus_tag="ACHKUN_003150"
     CDS             complement(8282..8875)
                     /gene="grpE"
                     /locus_tag="ACHKUN_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO6661365.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8998..9876
                     /gene="nadK"
                     /locus_tag="ACHKUN_003151"
     CDS             8998..9876
                     /gene="nadK"
                     /locus_tag="ACHKUN_003151"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO6661366.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9962..11623
                     /gene="recN"
                     /locus_tag="ACHKUN_003152"
     CDS             9962..11623
                     /gene="recN"
                     /locus_tag="ACHKUN_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO6661367.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11772..12113
                     /gene="bamE"
                     /locus_tag="ACHKUN_003153"
     CDS             11772..12113
                     /gene="bamE"
                     /locus_tag="ACHKUN_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6661368.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12175..12465)
                     /gene="yfjF"
                     /locus_tag="ACHKUN_003154"
     CDS             complement(12175..12465)
                     /gene="yfjF"
                     /locus_tag="ACHKUN_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO6661369.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12455..12892)
                     /gene="ratA"
                     /locus_tag="ACHKUN_003155"
     CDS             complement(12455..12892)
                     /gene="ratA"
                     /locus_tag="ACHKUN_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO6661370.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13063..13545
                     /gene="smpB"
                     /locus_tag="ACHKUN_003156"
     CDS             13063..13545
                     /gene="smpB"
                     /locus_tag="ACHKUN_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO6661371.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13760..14122
                     /gene="ssrA"
                     /locus_tag="ACHKUN_003157"
     tmRNA           13760..14122
                     /gene="ssrA"
                     /locus_tag="ACHKUN_003157"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3609 a         3808 c         3401 g         3536 t
ORIGIN      
        1 atatcgatgc atttcgagcg aagatggtgg ggatccctga atgcgttttt ctcaccgact
       61 gttcttactt ctgattctcc tgctgacggg agcaccaata ctcgctcagg agccttctga
      121 cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc agttacaccc gctggcctgc
      181 gctatcaggg ccaccaaagt tatgtatctt ttcatcttca cgttttagta ccgccctaca
      241 agagaatgcc gcgacatctt tgccttatct gcctgtcatc attcacactc aacaagaggc
      301 gatgatttca ggctgcaatg gtttttattt tggcaacgag tcaccgacat tccagatgga
      361 attaaccgaa caatatccgt caaaggcgtt attattaatt gccgaacaaa ataccgagtg
      421 cattattggt agcgcctttt gtctgatcat ccacaataat gacgtcagat ttgccgtaaa
      481 cctggatgcc ttatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa tgctcgcacg
      541 gaagaaaaat gatggataac gataattctc ttaataagcg ccccacgttt aaaagagcat
      601 tacgcaacat cagtatgacc agcatattta tcactatgat gctgatctgg ttgctgcttt
      661 ccgtgacctc ggtgctgacc ctgaaacagt acgcgcaaaa aaacctggca ctgacagcag
      721 caacaatgac ttacagtctg gaagcagctg tcgtttttgc cgatggccct gcagcaactg
      781 aaacactggc agcgctgggc cagcaagggc aattttcaac tgcagaagta cgtgataagc
      841 agcaaaatat tctggcgtcc tggcattaca cccgtaagga tccaggcgat actttcagca
      901 atttcataag ccactggctc ttccccgccc ccatcattca gccgattcgt cacaatggtg
      961 aaaccattgg cgaagtacgc ttaaccgctc gcgacagttc aatcagccat tttatctggt
     1021 tttcgctcgc cgtactgacc ggttgtattc tgctggcatc aggcatcgca attaccctca
     1081 cccgccattt gcacaatggc ctggtagaag cgctgaaaaa tatcaccgat gtcgtacatg
     1141 atgtgcgttc caaccgcaat ttttcccgac gagtttcgga agaacgtatc gctgagtttc
     1201 accgcttcgc tctcgacttc aacagtctgc tggatgaaat ggaagagtgg cagcttcgtt
     1261 tacaggccaa aaatgcgcag cttctgcgta ccgcgctaca tgacccatta accgggctgg
     1321 ctaaccgcgc agcgtttcgt agcggcatca atacgttgat gaacaattcc gatgcccgaa
     1381 aaacgtcggc gttactattt cttgatggcg ataatttcaa atatatcaat gatacctggg
     1441 gtcatgcgac gggcgataga gtcttgattg aaatcgcaaa acggttagct gaatttggcg
     1501 ggctgcgaca taaagcatac cgcctgggcg gcgatgaatt cgctatggtg ctctatgatg
     1561 tacagtcgga atctgaagtg cagcagatat gctcagcact gacacaaatc tttaatctcc
     1621 cgtttgatct tcataatggc catcagacca ccatgacatt aagcattggt tacgcgatga
     1681 ccattgagca cgcctctgcg aaaaaattac aagagcttgc cgatcacaat atgtatcagg
     1741 ccaaacacca gcgtgccgaa aagctggtga gataacaagg atatatcgat atgataaagc
     1801 acctggtagc acccctgatt ttcacctcac taatactgac tggctgccag tcccctcagg
     1861 gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta tggatttact gaatccgccg
     1921 gcgactggtc gctgggctta tcagatgcca ttctgttcgc aaaaaatgac tacaaattgc
     1981 tcccggaaag ccagcaacag atccaaacca tggcagccaa attggcctcg acagggctaa
     2041 cacatgcccg tatggatgga cacaccgata actatggtga agacagttac aacgaaggct
     2101 tatcattgaa acgggcgaat gtcgtggccg atgcatgggc tatgggtgga caaattccgc
     2161 gcagcaatct caccacacag ggtttaggaa aaaaatatcc catagccagt aacaagaccg
     2221 cccagggccg cgccgagaac cgccgcgtcg cagtggtgat tactacccct taaaacacca
     2281 gcaaacagat aaaaaaaggg ccagccaatt ggccagccct tcttaacagg atgtcgctta
     2341 agcgaaatct tagttaagac gctctttgat acgagcagcc ttaccagtac gctcacgcag
     2401 gtagtacagt ttagctttac gaacagcacc acgacgtttg acagaaatgc tgtcaactac
     2461 cggagagtga gtctggaaga cacgctcaac gccttcgccg ttggaaattt tacgaacagt
     2521 gaatgcagag tgcagaccgc ggttacgaat agcgataacc acgccctcga atgcctgcag
     2581 acgttttttg gaaccttcaa caacccatac tttcacttcc acggtatcac ccggacggaa
     2641 ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc ttaataatgt tgctcataat
     2701 ttaatctctt atcctgggta aactgatatc tcgggggctt acgccatccc atcatgttta
     2761 tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca accttgcttg ctcttcagtc
     2821 agagccaggt tttccagaag ttcaggtctt ctaagccagg tacggcccag cgactgtttc
     2881 aaacgccagc gacgtatctc ggcatggttg cccgacagta acactggcgg aacttccatc
     2941 ccttctaaca cctcaggccg cgtatagtgc gggcaatcca gcaatccttc agcaaaggaa
     3001 tcttccgttg ccgaggcttc atgtcccagt actcccggaa taaaccggga aacggagtca
     3061 atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt aatcgccgat tgaccattct
     3121 tcgtcaattt cggtttggat cacgcgctca tctatacctt cgtagcgacc gcacaccaga
     3181 atcaattttt gattcgttgc cagttcgctg acgcccgctt gatcaagctt gcgtccctgt
     3241 ggtgacagat aaatcacctt tgcgccttca cccgccgcgg cttttgctgc atgaatggcg
     3301 tcccgcaagg gttgcaccat cattaacatc cccggtccgc cgccgtaagg acgatcgtcc
     3361 acggtacggt gccggtcatg cgtgaagtcg cgaggactcc aactctggat gctcagcagg
     3421 ccatttttaa ctgcccggcc agttaccccg taatcggtaa ttgcgcggaa catttcagga
     3481 aacaggctaa ttatgccaat ccacatagcg ccgtctttta ccgtttatcc ggtggtttaa
     3541 aaaccaggat cccaatctac ttcgattgaa cgagtagtga gatcgacttt cttgataacc
     3601 tgcccatcga ggaacggtac gagacgttcc ttgataccaa acgcatcttt caggtttgcc
     3661 ttaatgacga gaacgtcatt agatccggtt tccatcatat cgacgacttt accgagatcg
     3721 tagccttcag tggttactac ctggcagccc atcaggtctt tccagtagta gtccccctct
     3781 tcaagctgag gcagctgcga tgaatccacg acaatttcac aattcgtcag caggttcgcc
     3841 gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt cctgattgtg gtgcttccag
     3901 ctttccagct ggacttgctg ccactgaccc gccttctgga taaaccaggg ctgatagtca
     3961 aaaatgcttt cggcgtcttc ggtggaagaa aacactctga gccacccacg aataccgtaa
     4021 gacgaaccca tttttcccaa aacgatggga tcaacaggtg cttgcgcggt gagttgtttg
     4081 ctcatcatga ccaccgtgac agattaagct gctttgttta cttctttgat cagcgcagca
     4141 acgcgatcag aaatagttgc gccctggcca acccagtgag cgatgcgatc cagatccagg
     4201 cgagtgcctt cttctttttc gctagcgatt gggttgaaga aaccaacgcg ctcgatgaag
     4261 cgaccgttgc gtgcattacg gctgtcagcg acaacaacct ggtagaacgg acgcttttta
     4321 gcgccgtgac gtgctaaacg aatagttacc ataacatcct cttgtgtgaa taaaacaacc
     4381 ggaccccatc gaggaacgga gtccggtgtc atattaaaag cccgaaaatt ttactcattt
     4441 ttgcgggaat tgcaatcaac agttgctaac tctgctgtaa aaggccgtcg gcggtgcagc
     4501 cagtttggtg ccggagtgcg cgcagtcacc ggagcgtaca cgcagtacgt gaggatgacg
     4561 agcacatccc ggtgccaaaa tggcaaacaa gccaggccga ttagcgacca gggaagcctg
     4621 ggggcatcat acccttcatg cttctcatca tcttcgccat tccgcctttc ttcattttct
     4681 tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttcacgtcc tgcacctgca
     4741 taccgcaacc ggcagcgata cgacgcttac gcgaaccttt gatgatttct ggcttagcgc
     4801 gctcttttat cgtcatcgag ttgatgatgg cttccatacg caccagcact ttatcgtcca
     4861 tctgcgactt aacattatcc gggatctgcc ccatgcccgg cagcttgccc atcaggctag
     4921 ccatgccgcc catatttttc atctggcgca gttgctcaag aaaatcgttg agatcgaagc
     4981 cgtcgccttt tttcagcttg ctggctaatt tctctgcctg cgcgcggtca actttgcttt
     5041 cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac gcgatgcggt
     5101 ccggatggaa cggctccagc gcctcagttt tctcgccaac accgagaaac ttgatcggtt
     5161 tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg tccactttgg
     5221 tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta ttggccgcat
     5281 cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc gacgcatgga
     5341 cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg gtatccacca
     5401 gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaacg atatctaccg
     5461 gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc agcgtctcaa
     5521 gctgtttgat tgccgccggg cgataaacgt cggcagaaac caccagcact ttcttcttgt
     5581 gcttctcgcg caggaactta ccgagtttac caacgctggt tgttttaccg gcaccttgca
     5641 ggcccgccat cagtacgacc gcaggcggtt gcgcagccag gttcagggtc tggttctctt
     5701 cgcccatcgc cgcaaccagt tcgttacgga ctattttgac gaactcctgc cccggcgtca
     5761 ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata aactcacgca
     5821 ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc agcgtatctt
     5881 ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg cgcgacaaac
     5941 gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg gccgcaatcg
     6001 cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt gttatgcaac
     6061 ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct caaactgtcg
     6121 acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc agtcttgcgc
     6181 tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct attatttctg
     6241 cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg cccgacggtg
     6301 atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg atgatctgta
     6361 cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc attgtctatg
     6421 cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac atcacccatc
     6481 tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc tatgccacgc
     6541 tgataattgc cgccctgtac gcgctacaac tggcgtggat tgattaccaa ctgaagaaca
     6601 agaagctggc gtttaaccaa gaaatgccgc cattgatgag tatcgagcgt aaaatgttcc
     6661 acatcacgca gattggcgtg gtgctactaa cgctcacgct ttgcactggc ctgttctaca
     6721 tgcacaactt atttagcatg gaaaatatcg acaaggccgt gctctctatc gtggcgtggt
     6781 ttgtctatat tgtgctgctg tggggacatt atcatgaagg atggcgtgga cgccgcgtcg
     6841 tctggtttaa cgttgcgggt gcagtcatcc tgacactggc ctacttcggc agccgaattg
     6901 tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca tttctactac
     6961 tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt ccgggtccga
     7021 aaccggaatg atgaccctca accgctatcg tctgcgacat atggcgaaac agggtaatcg
     7081 ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa gcctggtgtt
     7141 aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg ttgggatgcg
     7201 tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg tcgtactggt
     7261 ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag tcgcttatcc
     7321 gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct ggttgctgaa
     7381 tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg tggttagcgg
     7441 ctctttgagc aaagaagagt tgcgcactat cgtgcacgaa tcacgctcac aaatttcccg
     7501 tcgcaatcag gatatgctac tgtctgtgct cgatctggaa aagatgaccg ttgatgacat
     7561 catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga aatcgattct
     7621 gcgccaactc tcccactcac ctcacgggcg catcgtgctc taccgtgatt cgctggacga
     7681 cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga aaaaagagtt
     7741 caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgccgg aaggtacgcc
     7801 gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc tggtcgtcaa
     7861 cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag agattgtcgg
     7921 cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc aaaacgacgg
     7981 ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct ttaactggca
     8041 tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac tggaagagat
     8101 ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atagatattc tcgacgtaca
     8161 ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg agagtgtggc
     8221 ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac gaaaacagaa
     8281 attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga ccattcagcg
     8341 tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca gattccacca
     8401 ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg atcacttcaa
     8461 caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg ccttcaacca
     8521 tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc aggctatcaa
     8581 tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct ttttcaatat
     8641 ccagttcagt acgtcgacgc aggttttcca tttcggcttt tacacgcaaa atgccgtcac
     8701 gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt tcatcgcgcg
     8761 gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg tgctgatcca
     8821 tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta ctcatgaatt
     8881 tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga tcagtttcag
     8941 ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct cggaaaaatg
     9001 aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccccac tgcactgaca
     9061 acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat cgttgagcaa
     9121 caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc ggagattggg
     9181 caactagctg atctcgcggt agtcgttggt ggcgacggta atatgctggg cgcggcacgc
     9241 acactcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct gggtttcctg
     9301 actgaccttg accccgataa cgcccagcaa cagttagccg atgtgctgga aggccactac
     9361 atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg ccagaaacgc
     9421 atcagcaccg cgataaatga agtggtgctt catccaggca aagtggcgca tatgattgag
     9481 ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg actaattatt
     9541 tcgacgccaa caggctccac cgcctattcc ctctctgcag gcggtcctat tctgaccccc
     9601 tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc acgaccactg
     9661 gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa cgacctggaa
     9721 atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt cctgattcgt
     9781 cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt caacacatta
     9841 agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct ctttactgta
     9901 tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca ggaaaacgac
     9961 tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg agattgattt
    10021 tcatagcggc atgaccgtaa taactggcga gaccggcgcg ggtaaatcta ttgcaataga
    10081 tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta ccggcgctgc
    10141 tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggctc tgcgctggct
    10201 ggaagaaaat cagcttgaag acgggcatga atgtttgctt cgtcgagtga tcagcagcga
    10261 tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac tgcgcgaact
    10321 gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca ccaaacctga
    10381 gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctactgc aggaaatgac
    10441 cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc aacagttaag
    10501 tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac ttaacgaatt
    10561 taatccgcag cccggagagt ttgaacaaat cgacgaagag tacaaacgtc tggcgaacag
    10621 cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg gtgaagacgc
    10681 aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga ttggcatgga
    10741 cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga ttgctgaagc
    10801 cagcgatgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc gactatttga
    10861 acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg tcagccctga
    10921 ggcattgcca cagtattacc agtcgctact ggaagaacag cagcaactgg acgatcaggc
    10981 cgactcacaa gaaacgcttg cgctggcggt aacgaaacat catcagcagg cactggaaat
    11041 cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac agctgatcac
    11101 cgacagtatg catgcgctct caatgccgca tgggcagttt acgatcgatg ttaaatttga
    11161 cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa ccaccaaccc
    11221 aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt cccgcatcgc
    11281 actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga tttttgatga
    11341 agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc tgcgtcaact
    11401 cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg gatgtggtca
    11461 tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacgc atatgcaatc
    11521 cctgaataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca gtgaagtcac
    11581 acgtaataca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt tcctgcttca
    11641 cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt aaagtgatga
    11701 aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc ccgaaaagga
    11761 atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta ttgatgttga
    11821 ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac caggggaact
    11881 atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa caagttgcgt
    11941 acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg ttctatgtct
    12001 tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg acctttaaca
    12061 gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac taataataaa
    12121 gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg tctgttattt
    12181 attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa tgagagggcg
    12241 ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg gacggctgta
    12301 aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca agccactggc
    12361 gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc acgcgctgca ggtactgctt
    12421 ctcaggtagc gcataagcca cctcaacggc aattttacct ggcactgtaa acctctttcg
    12481 cacgaaccgt aaaagcctgg accatattag acgccagctc tttaaacacg cgaccaaagg
    12541 cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata cgacacgcct
    12601 cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc ccatccacca
    12661 gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac gttttgctga
    12721 tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac tccagaatcc
    12781 gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc actaactgat
    12841 acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc ataacaattt
    12901 ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa acgctatccc
    12961 ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca ggattgatat
    13021 ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa gaaaaaagca
    13081 cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga atactttatc
    13141 gaagaagagt tcgaagcggg acttgccctg caaggctggg aggttaaatc cctgcgcgca
    13201 ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc atttctgttt
    13261 ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga tcctacccgt
    13321 acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg cgtcaatcga
    13381 gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg caaagtgaaa
    13441 atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa agaacgcgaa
    13501 tggcaggtgg ataaagcacg tatcatgaaa aacgcccacc gttaaacctg cactccaatt
    13561 attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat cttattggct
    13621 atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat caggctacat
    13681 gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca taaatctggt
    13741 atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg aaacccaagg
    13801 tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc aaacgacgaa
    13861 aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc ctccgctctt
    13921 aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc tgcctggggt
    13981 tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg cagctggcaa
    14041 gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc ggacgcgggt
    14101 tcaactcccg ccagctccac cacttttgat aggactgcaa tcggacagcg gcaataaaaa
    14161 cagccactta cggactctga ccagacagag cgcagaccga gaaaagacaa aaaaatgcac
    14221 gtaaaatgca cgtgcacttt aaaagaaccc cagatctcac ggtctggttt ttttctgttt
    14281 gtaactaatg gtaacaaaaa cacatcacct cttggcgttc cttctccttg atactgttta
    14341 tccatacagt taaa
//
LOCUS       DAZFRJ010000118        14198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000118 DAZFRJ010000000
VERSION     DAZFRJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.119"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..1750
                     /gene="garD"
                     /locus_tag="ACHKUN_003158"
     CDS             179..1750
                     /gene="garD"
                     /locus_tag="ACHKUN_003158"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO6661372.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1899..2234
                     /gene="prlF"
                     /locus_tag="ACHKUN_003159"
     CDS             1899..2234
                     /gene="prlF"
                     /locus_tag="ACHKUN_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO6661373.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2234..2698
                     /gene="yhaV"
                     /locus_tag="ACHKUN_003160"
     CDS             2234..2698
                     /gene="yhaV"
                     /locus_tag="ACHKUN_003160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO6661374.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2753..3562)
                     /gene="agaR"
                     /locus_tag="ACHKUN_003161"
     CDS             complement(2753..3562)
                     /gene="agaR"
                     /locus_tag="ACHKUN_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO6661375.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3811..5091
                     /gene="kbaZ"
                     /locus_tag="ACHKUN_003162"
     CDS             3811..5091
                     /gene="kbaZ"
                     /locus_tag="ACHKUN_003162"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO6661376.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5114..5587
                     /gene="agaV"
                     /locus_tag="ACHKUN_003163"
     CDS             5114..5587
                     /gene="agaV"
                     /locus_tag="ACHKUN_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO6661377.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5598..6377
                     /gene="agaW"
                     /locus_tag="ACHKUN_003164"
     CDS             5598..6377
                     /gene="agaW"
                     /locus_tag="ACHKUN_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO6661378.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6367..7245
                     /gene="agaE"
                     /locus_tag="ACHKUN_003165"
     CDS             6367..7245
                     /gene="agaE"
                     /locus_tag="ACHKUN_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO6661379.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7263..7697
                     /gene="agaF"
                     /locus_tag="ACHKUN_003166"
     CDS             7263..7697
                     /gene="agaF"
                     /locus_tag="ACHKUN_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO6661380.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7694..8827
                     /gene="nagA"
                     /locus_tag="ACHKUN_003167"
     CDS             7694..8827
                     /gene="nagA"
                     /locus_tag="ACHKUN_003167"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6661381.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9178..10332
                     /locus_tag="ACHKUN_003168"
     CDS             9178..10332
                     /locus_tag="ACHKUN_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO6661382.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10345..11205
                     /gene="kbaY"
                     /locus_tag="ACHKUN_003169"
     CDS             10345..11205
                     /gene="kbaY"
                     /locus_tag="ACHKUN_003169"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO6661383.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11372..11848
                     /gene="agaB"
                     /locus_tag="ACHKUN_003170"
     CDS             11372..11848
                     /gene="agaB"
                     /locus_tag="ACHKUN_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO6661384.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11887..12690
                     /gene="agaC"
                     /locus_tag="ACHKUN_003171"
     CDS             11887..12690
                     /gene="agaC"
                     /locus_tag="ACHKUN_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO6661385.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12680..13471
                     /gene="agaD"
                     /locus_tag="ACHKUN_003172"
     CDS             12680..13471
                     /gene="agaD"
                     /locus_tag="ACHKUN_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO6661386.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13472..>14198
                     /locus_tag="ACHKUN_003173"
     CDS             13472..>14198
                     /locus_tag="ACHKUN_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO6661387.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLH"
BASE COUNT         3423 a         3609 c         3709 g         3457 t
ORIGIN      
        1 taaagcacag atttgcgctt taccttgccg ggcggcactg caatccctga aatgattgac
       61 attgatcaca tttctgcgtt taaactcctg acattcttat ttcacccaat gaagtcattt
      121 atttttaaat gagaccaggt cctcatttta ataacccctg gctggagaat atagtacaat
      181 ggccaacatc gaaatcagac aagaaacgcc aactgcgttt tatataaaag ttcacgacac
      241 agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc gttttccgga
      301 tggactggaa ttaattgaac atattcccca ggggcataaa gtcgcattgc tggacattcc
      361 ggctaatggt gaaattattc gttatggtga agtgattggt tacgccgtgc gtgcaatccc
      421 acgcggaagc tggctcgacg aatcaatggt tgtactaccg gaagcgccgc cgttacacac
      481 gctgccactg gcaaccaaag tcccggcccc cttaccgccg ctggaaggat acacctttga
      541 gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggca tcaccaccag
      601 cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa atcattgaac gcgatctgct
      661 accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg gctgtggcgt
      721 ggcgattaac gcaccggcgg cagttgtgcc tattcgtacc atccacaata tttcgctgaa
      781 ccctaacttt ggcggcgaag taatggtgat tggtctgggt tgtgaaaagt tgcagcctga
      841 gcgcctgctg gtcggcacgg atgatgtgca agctattcca gtagaaagcg ccagcattgt
      901 cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata ttttgcaggt
      961 cgccgaacgc catctacaaa aactgaatca acgtcagcga gaaacctgtc cggcttcaga
     1021 actggttgtc ggcatgcagt gcggtggcag cgatgccttt tctggcgtaa cggcaaaccc
     1081 ggcggttggc tatgcgtctg atctactggt gcgctgcggc gcaacggtga tgttctcaga
     1141 agtcacggaa gtgcgtgacg cgatccatct gctgacacca cgcgcagtga acgaagaggt
     1201 cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata tgggaaaaac
     1261 cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc ggtctggcaa acgtggtaga
     1321 gaaggcactc ggctccattg ctaaatcggg taaaagcgca attgttgaag tgctgtcgcc
     1381 cggtcaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca gcgattttgt
     1441 ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg
     1501 tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcgacccgca ccgagctggc
     1561 gaaccgctgg tttgatttaa tggatattaa cgcgggcact atcgccaccg gcgaagaaac
     1621 cattgaagag gtgggctgga agttgttcca ctttattctc gacatcgcca gcgggaagaa
     1681 gaaaaccttc tcggatcaat gggggctgca taaccagctg gcagtgttta acccggcacc
     1741 ggtgacctga tttcttttgg aacgtccctc gcaaaacatg gccttagtgc catgttttta
     1801 ttgtttaaag cccccacgtc cattaataat gcatttgcat taccttagtt caagcttata
     1861 atttgagcag aaaacaggct gtaaaaggac agtgaatcat gcccgctaat gctcgctctc
     1921 acgctgtact gaccactgaa tcaaaggtca cgatacgcgg acaaacaact atccccgcac
     1981 cagtgcgtga ggccttaaaa ctgaagccag gccaggacag cattcattac gaaattctgc
     2041 ctggtgggca agtctttatg tgccgactgg gagatgaaca ggaggatcat actatgaatg
     2101 catttttgcg ttttctggat gcagatatcc agaacaaccc gcaaaaaact cgtccattca
     2161 acattcaaca aggaaagaaa cttgtcgctg gcatggacgt caacattgat gatgagattg
     2221 gcgacgacga ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc
     2281 ctgttttcag gaaacctacg acgctttagt tgccgaagtc gagacattaa agggaaaaga
     2341 tcctgaaaat tatcagagaa aagccgccac aaagttattg gcggtagtcc ataaagtgat
     2401 tgaggagcat atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg
     2461 ctctgggaaa aataaagact ggtcacgggt aaaatttggt gctggtcgtt atcgtctctt
     2521 ctttcgttat agtgaaaaag agaaagtcat cattctggga tggatgaacg atgaaaacac
     2581 tctgcgcacc tacggtaaaa aaacagatgc ctataccgta ttcagcaaaa tgttaaaaag
     2641 aggacatcct cctgccgact gggaaaccct cacccgagaa acagaagaaa cccattgatg
     2701 gtgttcacaa tgcccgccga acgtattcgt gttgaccagc gggcaccctt tctcactccc
     2761 cgaccagaat cacttcaacc ccagcctttc gcagtccttc cagactatcc gcaggaatgc
     2821 cttcatcaac aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactggaac
     2881 gattgaattt actggaatcg gtgaccacga tgatccgttc cacaacttcg cacatccgac
     2941 ggtttaaacg ggcttcatct tcattatgtg tgctgacgcc gcgctccaga tcgatcgcat
     3001 ctacaccaag aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcgc
     3061 cgtaaaaaga ttgcgactga cggcgcaaat gcccgccggt catcagcagc tcaacgcctt
     3121 ccgcttccag caacgcatta gccacgttca taccgttggt catcgcaatt acgtcagtgt
     3181 gcttacgcat cagacgagca atctcaaaag tggtagtccc ggaatcgagg atcacccgat
     3241 gaccaggctg aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca
     3301 gcgcgctttt atcttccact gatggctcga ctgacggcgt cgtgctatcg cagatcaacg
     3361 cgccaccata ggcacgcaca gcgatcccct gcttttccag aaacgccaga tcattgcgga
     3421 tcgtcacagt agatacgcca tacaatgccg acagatcgtt aacctgcaca ctcccttgct
     3481 gtcgcagacg ctgaatgatc tgttctcgtc gctcgctggt gccagtcact cgcttctcac
     3541 ctgaagcgtc ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt
     3601 tgcctattaa cgcctttcta ttaagcaaat gcaagcccac cttgcccatt gacgcaagct
     3661 actctcgttt cagtgacttt cattatgttt cttttgtgaa tcagatcaga aaactattat
     3721 ctttcgtttt atttttatct caccatgacg cagtatcaac tgaaacaaaa cgaaagatta
     3781 atatcgcagt aatctgaact ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag
     3841 cacaaagcgg gcaaaacaaa tggaatttat gccgtttgtt ccgcacatcc gctggtgctg
     3901 gaggctgcaa tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc
     3961 aatcaggtag accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt
     4021 gtttgtcagc tcgccgactc gttgaatttc ccgcaggatg cgttgattct gggtggtgac
     4081 catctggggc caaaccgctg gcaaaacctg ccggccgctc aggcaatggc caatgccgat
     4141 gatttgatta aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga ttgcagcatg
     4201 tcctgtcatg acgatccgat tcccttaact gatgacatcg tggctgaacg cgccgcccgt
     4261 ctggcgaaag tggcggaaga aacctgtctt gaacactttg gcgaagccga tctggagtat
     4321 gtcattggta ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg
     4381 gtcaccacgc cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ctttgaaaag
     4441 caaggtttga atgccatctg gccacgcatc attgccctgg tggttcaacc cggcgtcgaa
     4501 ttcgatcaca ccaacgttat tgattatcag cccgccaaag cgagcgcctt aagccagatg
     4561 gtcgaaaact acgaaacgct gattttcgaa gcgcactcta ccgattatca aacgccgcaa
     4621 tcgctgcgcc agctggtgat tgaccacttt gccattctga aagttggccc agcgctgacc
     4681 ttcgccctgc gtgaagctct gttctctctg gcggcgattg aagaagaact ggtgccagcg
     4741 aaagcctgtt ctggtctgcg tcaggtgctg gaagacgtga tgctcgaccg cccggaatac
     4801 tggcaaagcc actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg ttatagctac
     4861 tcggatcgcg tgcgctatta ctggccggac agccagattg atgacgcttt cgctcatctg
     4921 gttcgtaatc tggcggattc accaattccg ctaccgctca tcagccagta tctgccgctg
     4981 caatacgtga aagttcgctc cggcgagctg caaccgacgc cacgggaact cattatcaac
     5041 catattcagg acatcctggc gcagtaccac acagcctgtg aaggccaata agcaaaacaa
     5101 agaggaacac gctatgccaa atattgtttt aagccggatt gatgaacgct tgattcacgg
     5161 tcaggtcggc gttcagtggg tcggatttgc gggggcaaat ctggtgctgg tagccaacga
     5221 tgaggttgcc gaagatccgg tacaacaaaa cctgatggaa atggtactgg cagaagggat
     5281 cgccgtacgt ttctggacgc tgcaaaaagt tatcgacaac attcatcgcg ccgccgatcg
     5341 acagaaaatc ctgctggttt gtaaaacacc cgccgatttc ctgacgctgg tgaaaggtgg
     5401 cgttccggtg aatcgcatta acgttggcaa tatgcactac gccaatggca aacaacaaat
     5461 cgccaaaacg gtttctgtgg atgcgggcga tatcgcagca tttaacgacc tgaaagccgc
     5521 tggggtggaa tgcttcgttc agggcgtccc gacagagcct gctgtagacc tctttaaatt
     5581 actttgaggg attcatcatg gaaatcagcc tgttgcaggc atttgcgttg ggcattatcg
     5641 cctttatcgc tggcctggat atgtttaacg gcctaacaca tatgcaccgc ccggtggtcc
     5701 tcggcccgtt ggtcgggctg gtacttggcg atctgcatac cggaatttta accggcggta
     5761 cgctggaact ggtgtggatg gggctggccc cgctggcggg cgcacagccg cctaacgtga
     5821 ttatcggtac tatcgtcggc acggcgtttg ccattactac tggcgtgaaa cccgatgtcg
     5881 cggtcggtgt cgccgtacct ttcgctgtcg cagtacagat ggggattacc ttcctgttct
     5941 cggtgatgtc tggcgtgatg tctcgctgcg accggatggc ggagaacgcc gatactcgcg
     6001 gcattgaacg cgtgaactat ctggcactgc tggcactcgg cactttctat tttctctgcg
     6061 ctttcctgcc tatctacttc ggcgcggaac atgcaaaaac catcattgat gtcctgccgc
     6121 aacgattaat cgacggcctc ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg
     6181 tgctgctgaa aatcatgatg aaaaacgtct acatccccta cttcatcctg ggctttgttg
     6241 ccgcagcctg gctcaagtta ccggtgctgg ctattgctgc cgctgcgctg gcgatggcgc
     6301 tgatcgacct gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag aaagaggaat
     6361 ttgaagatgg catctaatca aaccaccctg ccgaacgtca gtgaaaacga agaaacattg
     6421 ctgactggcg tcaatgaaaa cgtgtatgaa gatcagagca ttggcgcgga gctgacgaaa
     6481 aaagatatca accgcgtcgc ctggcgttcc atgctgttac aagcgtcttt taactacgaa
     6541 cgtatgcagg cttctggctg gttgtacggt ctgctgcctg ctctgaaaaa gatccacact
     6601 aataaacgcg acctggcgcg cgccatgaag gggcatatgg gtttcttcaa tacccatccg
     6661 tttctggtga catttgttat cggcattatc cttgcgatgg agcgttctaa gcaggacgtt
     6721 aacagtattc agagcaccaa aattgccgtc ggtgcgccgc tcggcgggat tggcgatgca
     6781 atgttctggc taacgctact gccgatttgt ggcgggattg gagccagtct ggcattgcaa
     6841 ggctccattc ttggcgcagt cgtctttatt ctgctgttca acgtggtaca cctggggctg
     6901 cgttttggtc tggcgcatta tgcttaccgc atgggcgtgg cggcgattcc actcattaaa
     6961 gcgaatacca aaaaagtcgg ccatgctgcg tctatcgttg ggatgacggt aatcggtgcg
     7021 ctggtggcaa cctatgttcg tttaagcacc acgctggaaa tcacagcggg cgacgcagtg
     7081 gttaagttac aggctgatgt tatcgacaaa ctgatgcctg ccttcttacc gttggtctac
     7141 accctgacca tgttttggtt ggtacgccgc ggctggagtc cgctgcgtct gattgcagtt
     7201 accgtggttc tcggcatcgt cggtaaattc tgccatttcc tttaaataca atgaggtatt
     7261 agatgttaag tattattttg acagggcatg gcgggtttgc cagcggcatg gaaaaagcaa
     7321 tgaagcaaat tctcggtgag caatcgcagt ttatcgccat cgattttccg gaaacctcaa
     7381 gcaccgcgct gcttacatcg cagcttgaag aggccatcgc ccaactggat tgtgaagacg
     7441 gcattgtttt tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg
     7501 cgctgcaaaa accgggctgt gaagtgatca ccggcaccaa tttgcaactg ctactggaga
     7561 tggtgctgga gcgcgaaggg ttaagcggtg aagaattccg tgtacaggcg ctggagtgtg
     7621 gacatcgtgg gctgaccagt ctggtggacg agttaggccg ctgccatgaa gaatgtccgg
     7681 tcgaggaagg gatatgacac acgttctgcg cgccagaagg ctgctgactg aagagggatg
     7741 gctcgatgac catcagttgc gtattgctga cggtgtcatc gcagcaatcg aaccgattcc
     7801 agtgggcgtg actgaacgcg atgcggaact gctctgcccc gcttacatcg acacccatgt
     7861 acacggtggt gcgggcgttg atgtaatgga tgacgcgccg gatgtactcg acaagctggc
     7921 aatgcacaag gcacgcgaag gtgtcggcag ttggttgccg actaccgtaa ccgcgccgct
     7981 taacaccatt catgcggcgc tgaaacgtat tgctcaacgt tgccaacgcg gcggacctgg
     8041 tgcgcaagtg ctggggagtt atctcgaagg accgtacttc acgccgcaga ataaaggcgc
     8101 gcatccgccg gaattgtttc gcgagcttga aattgccgag ctggatcaat tgattgccgt
     8161 ttctcagcac accttacgcg tggtagcgct ggcaccggaa aaagaggggg cattgcaggc
     8221 catccgccat cttaaacagc aaaatgtacg agtgatgctg gggcatagcg cggcgacctg
     8281 gcaacaaact cgcgccgcgt ttgatgctgg tgccgacggc ctggtgcatt gctataacgg
     8341 gatgacaggt ttacatcacc gcgaaccggg aatggttggc gcgggattaa cggacaagcg
     8401 cgcctggctg gaactgatag ccgatggtca tcatgtgcat ccggcggcga tgtcgctgtg
     8461 ttgttgctgt gcaaaagaga gaatcgtgct gatcaccgac gcgatgcagg cagccgggat
     8521 gccggatggt cgctatacgt tatgtggcga agaagtgcag atgcacggtg gcgttgtccg
     8581 taccgcgtcc ggtgggctgg cgggcagtac gctgtctgtt gatgcggcag tgcgcaacat
     8641 ggtcgagttg acgggcgtaa cgcctgcgga agccattcat atggcatcgc tgcatccggc
     8701 gcgaatgctg ggtgttgatg gtgttctggg atcgcttaaa ccgggcaaac gcgccagcat
     8761 cgttgcgctg gatagcgggc tgcatgtgca acaaatctgg attcagagtc aattagcttc
     8821 gttttgatat tttgctcctt tattgggcct tcacttcccc cgtaaggcct ttctttttct
     8881 ttcgttttga tctgtgcagc ggtgtcggat gcgacgctaa cgcgtcttat gcgacctacg
     8941 gtacgccgca tccagcaatc ggtgaacata gcgacgtcat tacgcaattc tttaatctcc
     9001 ccttgtcttt tctttcaatg atcacagatt tcgttttatt tcctttctct ccattgaact
     9061 ttcggtttct tttctataga ttttaatcaa cgaaagacat caccaagtga aatgaaacga
     9121 aaggcaagtg aaagcgacaa cgcccgacgt caagttcatc agactaagga ttgagttatg
     9181 ccagaaaatt acacccctgc tgccgccgca accggtacat ggactgaaga agagatccgc
     9241 catcagcctc gcgcatggat ccgttcactc accaacatcg acgcgctacg ttctgcgctc
     9301 aataacttcc ttgaaccgtt actgcgcaaa gagaatctgc ggatcatcct gaccggagcc
     9361 ggaacctcgg catttatcgg tgacatcatc gcgccgtggc tcgccagcca taccggtaaa
     9421 aacttcagcg ccgtaccgac caccgatctg gtcaccaatc cgatggacta cctgaaccca
     9481 gctcatccgc tgctgttgat ctccttcggt cgatccggca acagcccgga aagcgtcgct
     9541 gccgtggaac tggcaaatca atttgtacca gaatgctatc acctgccgat cacctgcaac
     9601 gaagcgggcg ctctttacca aaacgcgatc aacagcgata acgcgtttgc cctgctgatg
     9661 cccgcagaaa cgcacgatcg cggctttgcg atgaccagca gcattaccac catgatggcc
     9721 agctgcctcg cggttttcgc acctgagacg atcaacagcc aaaccttccg cgacgtggcg
     9781 gatcgttgcc aggcgatcct gacctcactg ggcgatttca gcgaaggtgt gtttggttac
     9841 gcaccgtgga aacggatcgt ttatctcggc agcggtggct tacagggcgc agcacgcgag
     9901 tcggcgctga aagtgctgga actgactgcg ggtaaactgg cggccttcta tgattccccg
     9961 accggattcc gtcatggccc gaaatcactg gtcgataacg aaacgctggt ggtggtattt
    10021 gtctcaagcc acccttacac ccgtcagtat gatcttgatc tgctggctga actccgccgt
    10081 gacaaccagg caatgcgcgt aatcgccatc gccgcggaaa gcaccgacat cgtcgctgcc
    10141 ggtccacata ttatcctgcc gccgtcacgt cactttatcg acgttgagca ggcattttgc
    10201 ttcctgatgt acgcccagac gtttgcactg atgcagtcgc tgcacatggg caatacgccg
    10261 gataccccat cagccagcgg caccgttaac cgcgtggtgc aaggcgtaat cattcatccg
    10321 tggcaggcat aagaggatcg cattatgagc attatctcca ctaaatatct gttacaggac
    10381 gcccaggcca atggctacgc ggtgcctgct tttaacattc ataacgccga gacgatccag
    10441 gcgatcctcg aagtgtgcag tgaaatgcga tcgccggtga tcctcgccgg aacgccgggg
    10501 acctttaaac atatcgcgct ggaagagatc tacgccctgt gcagcgccta ttccacaacc
    10561 tacaacatgc cactggcgct gcatctcgac caccacgaat cgctggatga tattcgccgt
    10621 aaagtccacg caggtgtgcg cagtgcaatg atcgacggca gccacttccc gtttgccgag
    10681 aacgtgaagc tggtgaaatc ggttgttgac ttctgccact cacaggattg cagcgtggaa
    10741 gcagaactgg gccgcctggg cggtgttgaa gatgacatga gcgttgacgc cgaaagtgca
    10801 ttcctgaccg atccacaaga agctaaacgc tttgtcgaac tgactggcgt cgacagcctg
    10861 gcggtagcga ttggtacggc gcacggctta tacagcaaaa cgccgaagat tgatttccag
    10921 cggctggcgg aaattcgtga agtggtggat gttcctctgg tgctacatgg tgccagcgat
    10981 gttccggatg aatttgtccg tcgcactatt gaacttggcg tcacaaaagt gaacgttgcc
    11041 acagaattaa aaatagcctt cgctggcgcg gttaaagcct ggtttgcgga aaatccgcag
    11101 ggtaatgatc ctcgttatta tatgcgcgtc ggaatggatg cgatgaaaga agttgtcaga
    11161 aataaaatta atgtctgtgg ttcagcgaat cgaatttcag cataatcatt agttttttat
    11221 gatttatccc aatgtacttc ccgattaata tcggggagtg ccttaatgga aaaggagatg
    11281 actaaacctt aataaataca tcagtacaat atcgcaacaa taatatatta aaaaaaatta
    11341 tattattcaa ctttatggtg aggattacac aatgagcagt ccaaatattc tcttaacccg
    11401 tattgataac cgtctggttc acggtcaggt tggcgtgacc tggacatcca ccatcggtgc
    11461 gaatctgctg gtagtcgtgg atgatgttgt cgctaacgat gatattcaac agaaattaat
    11521 gggtattacc gcagaaacct acggctttgg cattcgtttc tttactatcg aaaaaaccat
    11581 taacgtcatc ggcaaagctg caccgcatca gaagatcttc ctgatttgcc gtacgccaca
    11641 aacggtacgt aaattggtag aaggtggcat tgacctgaaa gatatcaacg tcggcaatat
    11701 gcatttctcg gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct
    11761 cgcggactta cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg
    11821 cgatcaaaaa gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta
    11881 ataaaaatgc atgaaataac cctacttcag ggattatccc tggcggcgtt agttttcgtt
    11941 ctggggattg atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc
    12001 ctaactggcg ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag
    12061 ttggctttcg ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg
    12121 ggtctgatga ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt
    12181 cttggcctgc cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc
    12241 tcattattta tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc
    12301 cggcttaact ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc
    12361 tgtacttacc tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg
    12421 acccacggct ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg
    12481 cgcgtaatgt tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc
    12541 tacatccagg tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat
    12601 gagtttttca atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat
    12661 gaagaagagg actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg
    12721 ctttcgttcg tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt
    12781 tacctgggcg atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag
    12841 cgcggcgatg aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct
    12901 gatggggtta ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct
    12961 caaagtggca ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt
    13021 gttgccgatt atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc
    13081 gattctattt ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt
    13141 cggttattca gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc
    13201 tcgctcggca accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca
    13261 tattaacgtg gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga
    13321 tttcttcgat aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta
    13381 cttcctgcgg gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc
    13441 tattgtttgt tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc
    13501 ctctttactt aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc
    13561 gttaagtgaa cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt
    13621 gatttgcctg gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat
    13681 ccaccagcag caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga
    13741 tctgccatta acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagct
    13801 gctggggctg cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga
    13861 gtgcgaacgg gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctagg
    13921 attggggaaa aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg
    13981 ccatatcagt caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg
    14041 ccccgtgact cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt
    14101 gttattggtg actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt
    14161 ctccaccgct atcccggctt cgtttttatg gctgcaca
//
LOCUS       DAZFRJ010000119        14137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000119 DAZFRJ010000000
VERSION     DAZFRJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.120"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..1314)
                     /gene="icd"
                     /locus_tag="ACHKUN_003174"
     CDS             complement(64..1314)
                     /gene="icd"
                     /locus_tag="ACHKUN_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO6661388.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1486..2139
                     /gene="rluE"
                     /locus_tag="ACHKUN_003175"
     CDS             1486..2139
                     /gene="rluE"
                     /locus_tag="ACHKUN_003175"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO6661389.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2149..2610
                     /gene="nudJ"
                     /locus_tag="ACHKUN_003176"
     CDS             2149..2610
                     /gene="nudJ"
                     /locus_tag="ACHKUN_003176"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO6661390.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2664..3770
                     /gene="mnmA"
                     /locus_tag="ACHKUN_003177"
                     /gene_synonym="trmU"
     CDS             2664..3770
                     /gene="mnmA"
                     /locus_tag="ACHKUN_003177"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO6661391.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3806..4447
                     /gene="hflD"
                     /locus_tag="ACHKUN_003178"
     CDS             3806..4447
                     /gene="hflD"
                     /locus_tag="ACHKUN_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO6661392.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4451..5821
                     /gene="purB"
                     /locus_tag="ACHKUN_003179"
     CDS             4451..5821
                     /gene="purB"
                     /locus_tag="ACHKUN_003179"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO6661393.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5989..6660
                     /gene="phoP"
                     /locus_tag="ACHKUN_003180"
     CDS             5989..6660
                     /gene="phoP"
                     /locus_tag="ACHKUN_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO6661394.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6660..8120
                     /gene="phoQ"
                     /locus_tag="ACHKUN_003181"
     CDS             6660..8120
                     /gene="phoQ"
                     /locus_tag="ACHKUN_003181"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO6661395.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8196..9317
                     /gene="roxA"
                     /locus_tag="ACHKUN_003182"
                     /gene_synonym="ycfD"
     CDS             8196..9317
                     /gene="roxA"
                     /locus_tag="ACHKUN_003182"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO6661396.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9463..10692)
                     /gene="pepT"
                     /locus_tag="ACHKUN_003183"
     CDS             complement(9463..10692)
                     /gene="pepT"
                     /locus_tag="ACHKUN_003183"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO6661397.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10942..12078
                     /gene="potA"
                     /locus_tag="ACHKUN_003184"
     CDS             10942..12078
                     /gene="potA"
                     /locus_tag="ACHKUN_003184"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO6661398.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12062..12925
                     /gene="potB"
                     /locus_tag="ACHKUN_003185"
     CDS             12062..12925
                     /gene="potB"
                     /locus_tag="ACHKUN_003185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO6661399.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(13158..13307)
                     /locus_tag="ACHKUN_003186"
                     /pseudo
     CDS             complement(13158..13307)
                     /locus_tag="ACHKUN_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            13431..13516
                     /locus_tag="ACHKUN_003187"
                     /pseudo
     CDS             13431..13516
                     /locus_tag="ACHKUN_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(13539..14129)
                     /gene="espM2"
                     /locus_tag="ACHKUN_003188"
     CDS             complement(13539..14129)
                     /gene="espM2"
                     /locus_tag="ACHKUN_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO6661400.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
BASE COUNT         3463 a         3584 c         3497 g         3593 t
ORIGIN      
        1 acctacataa ataaaacaac gggcgtgtta tacgcccgtt gcaatattaa acacatgtag
       61 tgattacatg ttcttgatga tcgcatcacc aaattctgaa catttcagca gtttagcgcc
      121 ttccatcaga cgctcgaagt cataggttac ggtcttcgca ttgattgcgc cttccatacc
      181 tttaacaatc aggtctgcgg cttcagtcca gcccatatgg cgtaacatca tctcagcgga
      241 gagaataata gagccagggt ttactttgtc ctgaccggcg tatttcggcg cagtaccgtg
      301 ggtggcttca aacagggcgc attcgtcacc gatgtttgct ccaggggcga taccgatacc
      361 gccaacctgc gctgccagag cgtcagaaat gtagtcaccg ttcaggttca tacaggcgat
      421 aacatcatat tcagccggac gcagcaggat ctgttgcagg aatgcatcag caatcacgtc
      481 tttaatgacg atctctttgc cggtgttcgg gtttttaact ttcagccacg ggccgccgtc
      541 gatcagttca ccgccaaact cttcacgcgc cagctggtag ccccagtctt taaacgcgcc
      601 ttcggtgaac ttcatgatgt tgcctttgtg caccagagtc acagagtcac gatcgttagc
      661 aattgcgtat tcgatcgctg cacgaaccag acgtttggtg ccttcttccg aacacggctt
      721 aataccgata ccacaatgtt ccgggaagcg aattttcttc acccccatct cttcacgcag
      781 gaatttaatc actttctcgg cgtcggcaga gtctgctttc cattcgatac ccgcataaat
      841 gtcttccgag ttttcacgga agataaccat atcggtcagt tcagggtgtt taaccgggct
      901 tggagtgcct tgatagtaac gtaccggacg caggcagatg tagagatcca gttcctggcg
      961 cagggcaacg ttcagagagc gaataccgcc accaacagga gtggtcagcg gacctttaat
     1021 ggcaacgcga tattcacgaa tcagatcaag agtttcagca ggcagccaga cgtcctgacc
     1081 ataaacctgt gtggattttt caccggtgta aatttccatc caggagattt tacgctcgcc
     1141 tttataggct ttctcgactg cagcgtcgac cactttcagc atggctgggg ttacatctac
     1201 accgattcca tcaccttcaa tgtaagggat aatcggattt tcaggaacgt tgagtttgcc
     1261 gttttgcagg gtgatcttct tgccttgtgc cggaacaact actttacttt ccattcacct
     1321 ctccttcgag cgctactggt ttgctcgtct gccaccacgt tgcatatgcg tttgcgtcct
     1381 gcgatacgga tgctttagag caattttttg ttaatgattt gtaattggct tgtcaatact
     1441 accctattgt ttgccgtcat gaaagatgcg cgttattagg ctataatgcg gcaattcata
     1501 atctctgaaa ataccatgca aaaaacttct tttagaaatc accaagttaa gcgattcagc
     1561 tcgcaacgtt ctaccaggcg taaacctgaa aaccagccca cgcgtgtgat cctgttcaat
     1621 aaaccctacg atgttcttcc gcagttcacc gatgaagccg gacgcaaaac attaaaagaa
     1681 ttcatcccgg ttcagggcgt ttatgcagca ggtcgtcttg accgcgatag cgaagggtta
     1741 ctggtgctga ccaataacgg cgcgttgcag gcgcgtttaa cccagccggg caaacgcacc
     1801 gggaaaatct attatgtgca ggtggaaggt attcccacac aagacgcact tgaagccttg
     1861 cgcaatggcg taaccttaaa tgatggccct accctgcccg caggcgcgga gctggttgac
     1921 gaacccgcgt ggttatggcc gcggaatcca ccaattcgtg aacgcaaaag tattcccacc
     1981 agctggctga agatcacctt atatgaagga cgtaatcgcc aggtgcgccg catgaccgcc
     2041 catgttggtt tccccacgct gcgactgatt cgctatgcga tgggagatta ctctttggat
     2101 aatcttgcca atggcgaatg gcgagaagtg acagattaag gatgcaaaat gtttaaaccg
     2161 cacgttaccg ttgcttgcgt ggtgcacgca gaaggcaaat ttttagtcgt tgaagagacg
     2221 attaatggta aagcgttatg gaaccaacct gccgggcatc tggaagccga tgaaacctta
     2281 gtggaagccg ccgcccgtga actgtgggaa gaaaccggca tcagcgcgca gccgcaacac
     2341 tttattcgta tgcatcagtg gattgcgcca gataaaacgc cgtttttgcg tttcctcttt
     2401 gccattgagc ttgagcaaat atgcccgacc cagcctcatg acagcgatat cgactgctgt
     2461 cgttgggtca gcgccgaaga aattttacag gcgtcaaatc ttcgttcgcc gctggtggcg
     2521 gaaagtatcc gttgttatca aagcgggcaa cgctatccgc tggagatgat tggcgatttt
     2581 aactggcctt ttacaaaggg tgtcatctaa aaggctgcgt gatagaatac gccgccttga
     2641 agttcaatgt cgtgagtgat ccaatgtctg aaaccgcaaa aaaagtaatc gtcggcatgt
     2701 ccggcggtgt cgattcctcc gtttctgcct ggctgttgca acaacaggga tatcaggtcg
     2761 aaggcctgtt tatgaagaac tgggaagaag acgacggtga ggaatattgc acagcggcag
     2821 cggatctggc tgatgcccag gctgtctgcg acaagctcgg cattgaactg cacaccgtta
     2881 actttgctgc cgagtactgg gacaacgtct tcgaactgtt ccttgccgaa tataaagccg
     2941 gtcgcacgcc gaatccggat attctgtgca acaaagagat caaatttaaa gccttcctcg
     3001 aatttgccgc cgaagattta ggtgccgatt atatcgctac cggtcattac gtacgtcggg
     3061 ccgatgtcga tggcaagagc cgcctgctgc gtggtctgga cagcaataaa gaccagagct
     3121 acttccttta tacgctcagc catgagcaga ttgcgcaaag cctgttcccg gtcggcgaac
     3181 tggaaaaacc gcaggtgcgt aagattgctg aagatcttgg tctggtcacc gcgaagaaaa
     3241 aagactctac cggcatttgc ttcattggcg aacgtaaatt ccgcgagttc ctgggccgtt
     3301 atctcccggc gcaaccgggc aaaatcatta ccgtcgatgg cgatgaaatt ggtgagcacc
     3361 aggggctgat gtatcacact ctcggtcagc gtaaaggtct gggtatcggt ggcaccaaag
     3421 aaggtaccga agaaccgtgg tatgtggtgg acaaagacgt cgaaaacaac attctggttg
     3481 tcgctcaggg ccatgaacac ccgcggctga tgtctgtcgg gttgattgcc cagcagttgc
     3541 actgggtcga tcgcgaacca ttcaccggca ctatgcgttg cacggtaaaa acccgctatc
     3601 gccagaccga tatcccttgc accgtcaagg cgctggacga tgatcgcatt gaagtgattt
     3661 tcgatgaacc ggttgccgcc gtgacgccgg gccagtctgc cgtcttctat aacggtgaag
     3721 tgtgcctcgg tggcggtatt attgagcagc gtctgccgct gccggtctga ttattatctt
     3781 tacttaaaac aaggaagcag tgaacgtggc aaagaattac tatgacatca ccctcgccct
     3841 ggccggtatt tgtcagtcgg cacgcctggt gcaacaactc gctcaccagg ggcattgtga
     3901 tgccgatgcg ctacacgtct cactcaacag tattattgat atgaacccca gctcgacgct
     3961 ggcggttttt ggcggtagcg aagccaacct gcgcgtcggg ctggaaaccc tgctcggcgt
     4021 gctcaatgcc agcagtcgcc agggcttaaa cgccgaatta acccgctaca cactcagctt
     4081 gatggtgctt gagcgcaaac tctcctcagc gaaaggcgcg ctcgacactc tgggcaaccg
     4141 gatcaacggc ctgcaacgcc agctcgaaca cttcgattta cagtccgaaa cgctgatgag
     4201 cgcgatggct gctatctatg ttgatgtgat cagcccgctt ggcccgcgca ttcaggtcac
     4261 cggttcccct gctgtactgc aaagcccaca agtgcaggcg aaagttcgcg caaccctgct
     4321 ggcaggcatt cgcgccgccg tgctctggca ccaggtcggc ggcggacgtc tgcaactgat
     4381 gttttctcgt aatcgcctga ccactcaggc aaaacaaatt cttgctcatt taaccccgga
     4441 gttgtgatct atggaattat cctcactgac cgccgtttcc cctgtcgatg gacgctacgg
     4501 cgataaagtc agcgcgctgc gcgggatttt cagcgaatat ggtttgctga aattccgtgt
     4561 acaagttgaa gtacgttggc tgcaaaaact ggccgcgcac gcagcgatca aggaagttcc
     4621 tgcttttgct gccgacgcaa tcggttacct tgatgcaatt gtcgccaatt tcagcgaaga
     4681 agatgccgca cgcatcaaaa ccatcgagcg taccactaac cacgacgtta aagcggttga
     4741 gtatttcctg aaagaaaaag tggcggagat cccggaactg cacgcggttt ctgaattcat
     4801 ccactttgcc tgtacttcgg aagatatcaa taacctctcc cacgcattaa tgctgaaaac
     4861 cgcgcgtgat gaagtgatcc tgccgtactg gcgtcaactg attgatggca ttaaagatct
     4921 cgccgttcag taccgcgata tcccgctgct gtctcgtacc cacggtcagc cagccacgcc
     4981 gtcaaccatc ggtaaagaga tggcaaacgt cgcctaccgt atggagcgcc agtaccgcca
     5041 gcttaatcag gtggagatcc tcggcaaaat caacggcgcg gtcggtaact ataacgccca
     5101 tatcgccgct tacccggaag ttgactggca tcagttcagc gaagagttcg tcacctcgct
     5161 gggtattcag tggaacccgt acaccaccca gatcgaaccg cacgactaca ttgccgaatt
     5221 gtttgattgc gttgcgcgct tcaacaccat tctgatcgac tttgaccgtg acgtctgggg
     5281 ttatatcgcc cttaaccact tcaaacagaa aaccattgct ggtgagattg gttcttccac
     5341 catgccgcat aaagttaacc cgattgactt cgaaaactcc gaagggaatc tgggcctttc
     5401 caacgcggta ttgcagcatc tggcaagcaa actgccggtt tcccgctggc agcgtgacct
     5461 gaccgactcc accgtgctgc gtaacctcgg cgtgggtatc ggttatgcgc tgattgcgta
     5521 tcaatccacc ctgaaaggcg tgagcaaact ggaagtaaac cgtgaccatc tgctggatga
     5581 gctggatcac aactgggaag tgctggctga gccaatccag acagttatgc gtcgctatgg
     5641 catcgaaaaa ccgtacgaga agctgaaaga gctgactcgc ggtaagcgcg ttgacgccga
     5701 aggcatgaag cagtttatcg atggtctggc gttgccagaa gaagagaaag cccgcctgaa
     5761 agcgatgacg ccggctaact atattggtcg agctatcacc atggttgatg agctgaaata
     5821 aactcgtatc agtgccggat ggcgatgctg tccggcctgt ttattaaaat tatccacttt
     5881 ttcttttttc actttacctc ccctccccgc tggtttattt aatgtttacc cccataaaca
     5941 cataatcgcg ttacactatt ttaataatta agacagggag aaataaaaat gcgcgtactg
     6001 gttgttgaag acaatgcgtt gttacgtcac caccttaaag ttcagattca ggatgctggt
     6061 catcaggtcg atgacgcaga agatgccaaa gaagccgatt attatctcaa tgaacattta
     6121 ccggatattg cgattgtcga tctcggattg cctgacgagg acggtctgtc actgattcgc
     6181 cgctggcgta gcaatgatgt ttcactgccg attctggtat taaccgcccg tgaaagctgg
     6241 caggacaaag tcgaagtatt aagtgccggt gctgatgatt acgtgactaa accgtttcat
     6301 attgaagagg tgatggcgcg aatgcaggca ttaatgcggc gtaatagcgg cctggcttca
     6361 caggtcattt cgctaccccc gtttcaggtt gatctctctc gccgtgaatt atctattaat
     6421 gacgaagtga tcaaactgac cgcgttcgaa tacaccatta tggaaacgtt gatacgcaat
     6481 aatggcaaag tggtgagcaa agattcgtta atgctccaac tctatccgga tgcggagctg
     6541 cgggaaagcc ataccattga tgtactgatg ggacgtctgc gcaaaaaaat tcaggcacaa
     6601 tatccgcaag aagtgattac taccgttcgc ggccagggct atttgttcga attgcgctga
     6661 tgaaaaaatt actgcgtctt tttttcccgc tctcactgcg ggtacgtttt ctgttggcaa
     6721 cggctgcggt agtactggtg ctttcgcttg cctacggaat ggtcgcgctg atcggttata
     6781 gcgtcagttt cgataaaact acgtttcggc tgttacgtgg cgagagcaat ctgttctata
     6841 cccttgcgaa gtgggaaaac aataagttgc atgtcgagtt acccgaaaat atcgacaagc
     6901 aaagccccac catgacgcta atttatgatg agaacgggca gcttttatgg gcgcaacgtg
     6961 acgtgccctg gctgatgaag atgatccagc ctgactggct gaaatcgaat ggttttcatg
     7021 aaattgaagc ggatgttaac gataccagcc tcttgctgag tggagatcat tcgatacagc
     7081 aacagttgca ggaagtgcgg gaagatgatg acgacgcgga gatgacccac tcggtggcag
     7141 taaacgtcta cccggcaaca tcgcggatgc caaaattaac cattgtggtg gtggatacca
     7201 ttccggtgga gctaaaaagt tcctatatgg tctggagctg gtttatctat gtgctctcag
     7261 ccaatctgct gttagtgatc ccgctgctgt gggtcgccgc ctggtggagt ttacgcccca
     7321 tcgaagccct ggcaaaagaa gtccgcgaac tggaagaaca taaccgcgaa ttgctcaatc
     7381 cagccacaac gcgagaactg accagtctgg tacgaaacct gaaccgattg ttaaaaagtg
     7441 aacgcgaacg ttacgacaaa taccgtacga cgctcaccga cctgacccat agtctgaaaa
     7501 cgccactggc ggtgctgcaa agtacgctgc gttctctgcg tagtgaaaag atgagcgtca
     7561 gtgatgctga gccggtaatg ctggagcaaa tcagccgcat ttcacagcaa attggctact
     7621 acctgcatcg tgccagtatg cgcggcggga cattgctcag ccgcgagctg catccggtcg
     7681 ccccactgct ggacaatctc acctcagcgc tgaacaaagt gtatcaacgc aaaggggtca
     7741 atatctctct cgatatttcg ccagagatca gctttgtcgg tgagcagaac gattttgtcg
     7801 aggtgatggg caacgtgctg gataatgcct gtaaatattg cctcgagttt gtcgaaattt
     7861 ctgcaaggca aaccgacgag catctctata ttgtggtcga ggatgatggc cccggtattc
     7921 cattaagcaa gcgagaggtc attttcgacc gtggtcaacg ggttgatact ttacgccctg
     7981 ggcaaggtgt agggctggcg gtagcccgcg aaatcaccga gcaatatgag ggtaaaatcg
     8041 tcgccggaga gagcatgctg ggcggtgcgc ggatggaggt gatttttggt cgccagcatt
     8101 ctgcgccgaa agatgaataa atatgtccat acttcacgca ttacgttaag catccgttat
     8161 aatcggttgc agataccagc ctgtggatgc ttaacatgga ataccaactc actcttaact
     8221 ggcccgattt tcttgaacgt cactggcaga aacgcccggt ggtgttaaaa cgcggcttta
     8281 ataattttat tgacccgatc tctccagacg agttggcggg tctggcgatg gaaagcgaag
     8341 ttgacagtcg actggtcagt caccaggatg gcaaatggca ggtcagccac ggcccgttcg
     8401 aaagctacga tcatctcggt gaaaccaact ggtcattact ggtacaggca gtgaaccact
     8461 ggcatgagcc gaccgccgcg ctgatgcgac cgttccgtga actaccggac tggcgtattg
     8521 atgatctgat gatctctttt tctgtaccgg gcggcggcgt cggcccgcat ctcgatcagt
     8581 acgacgtgtt tatcattcag ggtaccggtc gtcgtcgctg gcgagtgggc gaaaagctgc
     8641 aaatgaaaca gcactgtccg cacccggatc tgttacaggt cgatccgttc gaagccatca
     8701 tcgatgaaga gctggagcct ggcgatattc tttatattcc gccaggattc ccgcatgaag
     8761 gctacgcgct ggaaaatgcg atgaactatt ccgtgggttt tcgcgcgcca aatacgcggg
     8821 aattaattag cggatttgcc gattatgtgc tgcaacgtga actgggcggc aactactaca
     8881 gcgatccgga tgttccacct cgcgctcatc ctgcggacgt tctgccgcaa gagatggata
     8941 aactgcgtga gatgatgctc gaattgatca accagccgga acactttaag caatggtttg
     9001 gcgagtttat atcccagtca cgtcatgaac tggatatcgc gccgccggaa ccgccttatc
     9061 agccagatga aatctacgat gcgctgaaac aaggtgaagt gctggtgcgc ctgggtggtc
     9121 tgcgcgtatt gcgcattggc gacgacgtgt atgccaatgg tgagaagatc gattccccgc
     9181 accgtccggc actggatgca ctcgccagca acattgcgct gactgcggag aattttggcg
     9241 atgcgctgga agatccgtca ttcctcgcga tgctcgcggc gctggtcaat agcgggtatt
     9301 ggttcttcga agggtaagtt tggatttaag ccggatgcgg cgctgattgc gccttatcgt
     9361 gcctacatgc actgaacgcc tgtaggcacg ataagtatgt ggaccagtat caatataacg
     9421 gtttcatccc ggtaattggc agtccacata cgcatcgggc aatcacgact tccgttgcgc
     9481 cgttaactcg gcaatacgga caatcacctg caccgctttt tccatacctt ccagagtcac
     9541 aaactcatgc ttaccatgat agttgtaacc gccagtgaac aggttcgggc acggtaatcc
     9601 cataaacgac aactgcgcgc cgtcggtacc gccgcggatc ggtttcagtt ccggttcaat
     9661 atcgcagtca cgcatcgcct gctgggcgat atcgataata tgcggatgct caaccacttt
     9721 ctcgcgcata ttgtagtaac tgtcttcaat caccagttca atgtagcaat caggatgtaa
     9781 ccctttgccc acttttttgg cgatctccat cattttacgt ttacgcgctt caaactgttt
     9841 acggtcgaaa tcacggatga tgtagtgcat atcggctcgg tcaacggtgc ctttcatgct
     9901 cgccaggtga tagaaacctt catagccttc tgtcatttcc gggctttcat ccgccggaac
     9961 ttccgcatga atacgtgccg ccagcgacag cgcatttacc atcactcctt tcgccgtgcc
    10021 cggatgaaca ttgttaccga caattttgat attgaccgat gcggcgttga agttttcaaa
    10081 ctccagttcg cctacgccac caccgtcaac agtgtaagcc cagcgggcat cgaaggcatc
    10141 aacatcaaaa tgtttcgccc ctttgcccac ttcttcatcc ggggtaaagg cgacgcgaat
    10201 atcaccatgc ggaatgtttt tctgttgcaa taccgccagc gcggtcatga tttctgcaat
    10261 acctgctttg tcatcggcac ctaacaaggt tttaccatcg gtggtaatca acgtctgacc
    10321 cagtagctga tgcagcaccg ggaacataac cggtgataaa acttcatcgc cgatacccag
    10381 cgcaatatcg ccaccgcgat agttttcaac aatttgcgga ttcacatttt tgccgctgca
    10441 atccggtgag gtatccacat gagaaataaa gccaatcgcc gggatatcgc cagggacgtt
    10501 agccggtaac gtcgccatca aagtgccctt ctcacttaag gtcacattga taagccccat
    10561 ctcttcgagc tgctctttca gcagatgcaa taacttccat tggccttccg tgctgggaac
    10621 ctgtctcacc cctgcttttg attgggtatc cagagacacg tagttcaaaa atcgctcaag
    10681 tagtttatcc atgtagtcac cctcactttt tgtgacaaca ttattaagaa gcaagaaaag
    10741 acaaatattg cgtcaggtca cttttacccc tgcaagcggg aatatttatc agcattacct
    10801 taatgaatat aaagctaagc cagtaattca caacaaagat tggcgattca agcggtttgc
    10861 cgtagaatta ccgccctcat taagagtcac caaggtggtt aaccacaaac cccgcatcgg
    10921 taagccatcc gttgcgttta catgggacag agtaaaaaat tgaataaaca accgagttcg
    10981 ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct ttgatggtaa agaggtcatt
    11041 ccccagctgg atctgactat caacaatggc gagttcctca cgctgcttgg cccttctggc
    11101 tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg aaactgttga ttccggacgc
    11161 atcatgctgg ataacgagga catcacccac gttccggcgg aaaaccgcta tgtgaacact
    11221 gttttccaaa gctacgcact tttcccccac atgaccgtgt tcgaaaatgt ggcctttggg
    11281 ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc gcgtgatgga agccctgcgg
    11341 atggtgcaac tggaaacctt cgctcaacgt aaaccgcatc agctctctgg tggtcaacag
    11401 caacgcgtcg ccattgctcg cgcggtggtt aacaagcctc gtctgttgtt gctggatgag
    11461 tcgctctcag cgctggatta caaactgcgt aagcaaatgc agaacgagct gaaagcgtta
    11521 cagcgtaagc ttggtattac cttcgtcttt gtcactcacg atcaggaaga agcgctcacc
    11581 atgtcagaca ggattgtggt gatgcgcgag ggtcgtattg agcaagatgg tacaccgcgc
    11641 gaaatttacg aagagccgaa aaacctgttt gttgccggct tcattggcga aatcaatatg
    11701 tttaacgcca ctgtcatcga acgtctagac gagcagcgcg tacgcgccaa cgttgaaggc
    11761 cgcgaatgta atatctacgt taacttcgcc gttgaaccgg ggcaaaaact gcatgttctg
    11821 ctgcgcccgg aagacttacg tgttgaagag attaacgacg acaaccacgc tgaagggctg
    11881 attggttacg ttcgtgagcg taactacaaa ggcatgacgc tggagtcggt tgttgaactg
    11941 gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg aagacgatcc tgactttgac
    12001 cactctctcg accaaaaaat ggccattaat tgggtagaaa gctgggaggt cgtactggct
    12061 gatgaagaac acaagtaagt tccagaatgt agtgattgtc actattgtcg gttggcttgt
    12121 gttgtttgtc tttctgccca acctgatgat cattggcacc agctttttga cccgcgacga
    12181 cgccagtttc gtaaaaatgg tctttacgct ggataactac acgcgtctgc tcgatccgct
    12241 ctattttgaa gtgctattgc actcgctgaa tatggcgctg atcgccaccc tcgcctgcct
    12301 ggtgctgggc tacccgtttg cctggtttct ggcgaagctg ccacacaagg tgcgtccgct
    12361 gctgctgttt ctgctgattg ttcccttctg gaccaactca ttgattcgta tctacgggct
    12421 gaaaattttc ctcagcacca aaggctatct caacgagttt ttgctctggc tgggcgttat
    12481 cgacacacca atccgtatca tgttcacgcc cagtgcggtg attatcggtc tggtttacat
    12541 tctgctgccg tttatggtga tgccgctgta ctccagtatc gaaaaactgg ataagccgtt
    12601 gcttgaggcg gcgcgcgatc tcggtgccag caagttacag acttttatcc gtatcattat
    12661 tccactgacg atgccgggaa ttattgccgg atgtctgctg gtgatgctgc cagcgatggg
    12721 cctgttctat gtatccgacc tgatgggcgg tgcgaaaaac ctgctgatcg gtaacgtcat
    12781 caaggtacag ttcctcaata ttcgtgactg gccatttggt gcagccacca gcattacgct
    12841 gactatcgta atgggcctga tgttgctggt ttactggcgc gcttctcgtc tgctgaataa
    12901 gaaggtgaac gaattagatg attaatcctt tatattcaat ttattaggtt tgtttttgag
    12961 tgattttgtc gtttaaggta acggcgttat agatcctatc aaaatcaatc aattgcattc
    13021 tggttttgga gaaagatttt tcgagagctt cggtctttaa ctaaaactga ttgcgtataa
    13081 tcgcaatacc ccctccccga ctatctgaat ggtcggggag gcacttcaag cacaaaatat
    13141 catccctgct ccgttgatta ctctacaggt gaagtcggcc actcaatatc aggtgctgct
    13201 gatgtatcaa cacgattcaa caacacccga tactttttcc aggcttcccg caatgaagtc
    13261 tcttcctcaa ttacgatctc cagatctgca gcatcctgaa gtggggcgca ccacgcaaac
    13321 atcacaaaat attgtcgttg tgatggacct gtgcaatagt gaatcacgct acgtttctgt
    13381 gtacaacgta ctgtagtttt ctttacgatt tatagctgta ctggtgagcc atgagtaatc
    13441 tgaatccatg catggcgtgt ggtgcctgtt gtgcattttt ctgcgtctct tttactggtc
    13501 cgaagccgat gatgctgaag cagtcctctc ctctagcctc atccctgtat agcacgcgtc
    13561 aagtaagctg ggatatcatt tcgtagtttt acaatatcaa tatccgaatc gatatgtttc
    13621 tggtaaaact tgtccgcaag caagtttgct atggtatctg atgtacgatt ttttacatcc
    13681 cggctcatac catcacaaag agcatccatt ttcttgccaa aagaagcact ccccattatt
    13741 acatgattaa tagaactttg tgctgcctta acatcaaggg ttagttgatt ctcctggctc
    13801 acaagagaaa agactctttg cctctcttca gtggaaattt ttgcattttt ttgaaaacag
    13861 taattatcaa tctcctggtt gatcaccctt gagggatata ctatgcgttc atcttttatc
    13921 cacccatcaa tatctttagc actctgcctg ttcactgtat ccagtatatt tctgctaaat
    13981 ttgcgaacag aaaagctaat agtccgttct ccaattgaac attttacgac ctcatcattc
    14041 ttccctcgga aagagttcct aaaggagcct tctctatggc aacttatacc aaaagagctg
    14101 aaagacgtac ctgaagtatt catcggcatt ataattc
//
LOCUS       DAZFRJ010000120        13837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000120 DAZFRJ010000000
VERSION     DAZFRJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.121"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..109
                     /locus_tag="ACHKUN_003189"
                     /pseudo
     CDS             20..109
                     /locus_tag="ACHKUN_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            133..4647
                     /locus_tag="ACHKUN_003190"
     CDS             133..4647
                     /locus_tag="ACHKUN_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO6661401.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4648..6297
                     /gene="yfaQ"
                     /locus_tag="ACHKUN_003191"
     CDS             4648..6297
                     /gene="yfaQ"
                     /locus_tag="ACHKUN_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO6661402.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6302..7078
                     /gene="yfaP"
                     /locus_tag="ACHKUN_003192"
     CDS             6302..7078
                     /gene="yfaP"
                     /locus_tag="ACHKUN_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO6661403.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7353..10202
                     /gene="rcsC"
                     /locus_tag="ACHKUN_003193"
     CDS             7353..10202
                     /gene="rcsC"
                     /locus_tag="ACHKUN_003193"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO6661404.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10288..10938)
                     /gene="rcsB"
                     /locus_tag="ACHKUN_003194"
     CDS             complement(10288..10938)
                     /gene="rcsB"
                     /locus_tag="ACHKUN_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO6661405.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(10955..13627)
                     /gene="rcsD"
                     /locus_tag="ACHKUN_003195"
     CDS             complement(10955..13627)
                     /gene="rcsD"
                     /locus_tag="ACHKUN_003195"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO6661406.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         3317 a         3421 c         3724 g         3375 t
ORIGIN      
        1 atttttgtaa tttactctcc ttctgacatg gaaggacacc cgatggatac ccaacgattc
       61 caatcccaat ttcattggca tttatcgttt aaattttctg gcgcgatagt agcgtgcttg
      121 tccctgtctc ttgtgggaac agggcttgct aatgctgatg attcgcttcc ttccagtaac
      181 tatgcgccgc ccgccggggg aacattcttt ttgcttgctg acagcagttt tagcagcagt
      241 gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt atcggcgcta tcagatggaa
      301 gagtacggcg gcgtggacgt tcgcctgtat cgtattcctg acccgatggc atttttgcgc
      361 cagcagaaaa acctgcatcg cattgtggtg caaccgcaat atctgggcga cgggctgaac
      421 aatacgctga cctggctgtg ggataactgg tacggcaaat ctcgccgcgt aatgcagcgt
      481 actttctctt ctcagtcacg gcagaatgtg actcaggcat tacccgaatt acatctcggc
      541 aatgccatta ttaaaccttc ccgttatgta cagaacaacc agttttcccc gctgaaaaaa
      601 tatcccttgg tggaacagtt ccgttatcca ctatggcagg ctaaaccggt cgagccgcag
      661 caaggggtaa aactggaagg cgcatccagc aatttcatct cgccgcagcc gggtaacatt
      721 tatattcctc tcggccaaca agagccggga ctgtacctcg tcgaggcgat ggttggtggg
      781 tatcgggcga cgacggtggt gtttgtttcc gataccgtgg cgcttagcaa agtgtcaggc
      841 aacgagcttc tggtgtggac cgcgggtaaa aaacagggtg aagcgaagcc cggctcagag
      901 atcttgtgga ctgacggtct tggcgtgatg acccgcggtg tgaccgatga cagcggtacc
      961 ttgcagttac aacatatatc gccagaacgt tcatacattc tgggtaagga tgctgaaggc
     1021 ggtgtttttg tctccgagaa cttcttctac gaaagcgaaa tctacaacac ccgcttgtat
     1081 atttttaccg atcgcccgct atatcgcgca ggcgatcgtg tcgatgttaa agtgatgggc
     1141 cgcgagttcc acgatccgtt gcattcatcc cccatcgtca gcgccccggc gaagctttcg
     1201 gtgctggacg ctaacggcag tctgttgcaa accgtcaatg tcacgctgga tgcgcgcaat
     1261 ggcgggcagg gaagtttccg cctgccagaa aatgccgtag ccggaggtta tgagttacgt
     1321 cttgcttacc gcaatcaggt ctatagcagc agttttcgcg tggcaaacta catcaagcca
     1381 catttcgaga ttggtttagc tctcgacaaa aaagagttca aaactggcga agcggtcagc
     1441 ggcaaactgc aactgcttta cccggatggt gagccggtaa aagatgcccg cgtgcagtta
     1501 agtttgcgcg ctcagcaatt atcaatggtc ggtaacgatt tgcgttatgc cggacgtttt
     1561 cccgtgtcgc tggaaggcag cgaaacggtg tccgacgcca gcggtcatgt ggcgttaaat
     1621 ctccccgccg ccgataaacc gagccgctat ttgttaaccg tctccgccag tgacggcgcg
     1681 gcgtatcgcg tcaccaccac caaagagatc ctcattgaac gcggcctggc gcattactct
     1741 ttaagtaccg ccgcacaata cagtaatagc ggcgagtcgg ttgtgttccg ttatgccgcg
     1801 ctggaatctt caaaacaggt tcctgttacg tatgaatggt tgcgtctcga agaccgcacg
     1861 agccatagcg gagagctacc gtcaggcggc aaatccttta ccgtcaattt cgctaaacct
     1921 ggcaactaca atctgacatt acgcgataaa gacggcttaa ttctcgctgg gttaagtcat
     1981 gccgtcagcg gtaagggcag cacggcgcat actggtacgg tagatatcgt ggcggataaa
     2041 acgctgtacc agccaggcga aactgcgaag atgctgatta cctttccgga gccaattgat
     2101 gaagcattat tgacgctgga acgcgatcgc gtggaacagc agtcgctgct ttcgcatccg
     2161 gcaaactggc tgacgttaca acgtttaaac gatacccagt atgaagcccg ggttccagtg
     2221 agcaattcct ttgcgcctaa catcactttt tcggtgctgt atacccgtaa tggtcagtac
     2281 agttttcaga acgccgggat caaagttgcc gttcctcagc ttgatatccg ggtgaaaacg
     2341 gacaaaaccc attaccagcc tggtgaactg gtcaatgtcg aattaacctc gtcgctgaaa
     2401 ggtaaacctg tttctgcgca gctaacggta ggcgtggtcg atgaaatgat ctacgcgctg
     2461 caaccagaaa tcgcgccgaa tatcggcaaa tttttctatc cgctggggcg taacaatgtg
     2521 cgtaccagct ccagtctgtc gtttatcagc tacgaccagg cgctctccag cgagccggtt
     2581 gcgcctggcg caactaaccg cagcgagcgg cgagtaaaaa tgcttgaacg tccacggcgt
     2641 gaagaggtgg ataccgcggc atggatgccg tcactcacaa ccgataaaca aggcaaagcg
     2701 tatttcacgt tcctgatgcc tgattcgtta acccgctggc gtatcaccgc gcgtgggatg
     2761 aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc gttcggaaaa aaatctctac
     2821 atgaagtgga gtatgccaac ggtgtatcgc gtgggcgaca aaccggcggc aggactgttt
     2881 atcttcagtc agcaggataa cgaaccggtg gcgctggtga ctaaatttgc aggcgctgag
     2941 atgcgccaga cgctgacgct gcacaaaggg gcgaattata tttcgctgac gcagaacatt
     3001 cagcaatctg gcttgttaag tgcagaactg caacaaaatg ggcaagtgca ggacagcatt
     3061 agcacaaaac tgtcttttgt ggacaacagc tggcccgttg aacagcagaa aaatgtcatg
     3121 ctcggcggtg gcgagaacgc gctgatgttg cccgagcagg cgagtaatat ccggctacaa
     3181 agtagtgaaa cgccgcagga gattttccgc aacaatcttg atgcgttagt cgatggacca
     3241 tggggtggcg tgatcaacac cggtagccgt ctgatcccgc tcagtctcgc ctggcgttcg
     3301 cttgccgatc atcaaagtgc cgccgctaac gacattcgtc agatgattca ggataaccgt
     3361 ctgcggctga tgcaactggc ggggcccgga gcgcgcttta cctggtgggg tgaagatggc
     3421 aatggtgacg ccttccttac ggcatgggca tggtacgccg actggcaggc cagccaggcg
     3481 ctcggcgtaa cgcaacaacc ggaatactgg cagcatatgc tcgacagtta tgccgagcag
     3541 gcggataaca tgccgttatt gcatcgggcg ctggtgctgg cgtgggcaca ggagatgaat
     3601 ctgccgtgca aaacgttgtt gaaagggttg gatgaagcta tcgcccggcg cggaactaaa
     3661 gatgaagatt tctctgagga agacatccgc gatatcaatg acagcctgat cctcgataca
     3721 ccggaatctc cactggcaga tgcggtggca aacgtcttaa ccatgacgtt gctgaaaaaa
     3781 gcgcagttga agtccacggt gatgccacag gttcagcaat atgcgtggga taaagcggca
     3841 aacagcaatc agccgctggc gcacacggtt gtgctgctca atagcggtgg tgacgctacc
     3901 cagacggccg ctattttaag tggtttgacc gctgagcaat ccactattga gcgcgcgctg
     3961 gccatgaact ggctggcgaa atatatggcg acaatgcctc cagttgtttt gcctgcgcct
     4021 gcgggcgcat gggctaaaca taagttaact ggagggggcg aagactggcg ttgggttggt
     4081 cagggcgtgc cggacattct ctcttttggt gacgaattat cgccgcaaaa tgtgcaggtc
     4141 cgctggcgtg agccggcaaa aatggctcaa caaagtaaca ttccggtgac cgttgaacgc
     4201 cagttgtatc ggcttatccc tggtgaagaa gagatgagct ttattctgca accggtgacc
     4261 agcaatgaga ttgacagcga tgcgctgtat ctcgatgaaa ttacgcttac cagcgagcag
     4321 gatgcagttc tgcgctatgg tcaggtggaa gtaccgctcc cgccgggagc cgacgttgag
     4381 cgcacaacat ggggcatttc agtcaataaa cccaacgccg cgaaacagca ggggcaattg
     4441 ctggaaaaag cgcgtaatga aatgggcgaa ctggcctata tggtgccggt gaaagaactg
     4501 acgggaacgg tcactttccg ccatttgctg cgcttctcgc aaaaagggca attcgttctg
     4561 cctcctgctc gttatgtgcg ttcctatgca cctgcgcagc aaagtgttgc ggcagggagt
     4621 gaatggaccg ggatgcaggt gaaataagtg aactggcgca gaatcgtctg gctattggcg
     4681 ttggttactc tgccaacgct tgcggaggag acgccgttac aactggtgct gcgcggtgcg
     4741 cagcacgatc aactgtatca gctgtcgtca tcaggcgtga ccaaagtctc agcattacct
     4801 gactcgctca ccacgccgtt agggagtttg tggaaactgt atgtttacgc ctggctggag
     4861 gatacccatc aaccggagca gccctaccag tgtcgcggaa actccccgga agaggtttat
     4921 tgttgtcagg ctggggagag cataacgcgc gataccgcgc tggtacgttc ctgtgggctc
     4981 tattttgcgc cgcagcggtt gcatattggc gcagatgtgt gggggcaata ctggcaacag
     5041 cgtcaggcac ctgcgtggtt agcgtctctt acaacgctga agccagaaac ctcggtaacg
     5101 gttaaatcgc tgctcgattc attagcgacg ttacctgcgc aaaacaaagc tcaggaagtc
     5161 ttactggatg tggtgctgga cgaggcgaaa atcggtgtcg catccatgct cggtagtcgg
     5221 gtacgggtga aaacctggag ctggtttgcc gatgataaac aggaaatccg ccagggtggt
     5281 tttgctggct ggttaaccga tggtacaccg ctgtgggtta ctggcagtgg caccagcaaa
     5341 acggtactaa cccgttatgc gacggtgctt aatcgggttt tgccagtgcc gacgcaggtt
     5401 gccagtgggc aatgtgtaga ggtggagctg tttgcccgct atccgctgaa gaaaatcaca
     5461 gcggaaaaga gtacgacggc agtaaaaccc ggcgtactta atgggcgcta tcgtgtgacg
     5521 tttactaatg gtaaccacat tacgtttgtc agccacggtg agacgacctt actgagcgaa
     5581 aagggcaaac tgaaattgca gtcgcatctc gatcgtgaag agtacgttgc cagagttctg
     5641 gatcgcgagg cgaaaagtac gccccctgag gcagcgaaag ccatgaccgt ggcgattcgc
     5701 acttttttac agcaaaacgc caatcgggaa ggcgattgcc tgaccatccc cgacagcagc
     5761 gccacgcagc gtgtttctgc ttcgccagcg accacggggg cgcgaacgat gaccgcctgg
     5821 acgcaggatc tcatctatgc aggcgatccg gttcattatc atggcagtcg agctaccgaa
     5881 gggacacttt cctggcggca agccacggcc caggcggggc agggagagcg ttacgaccag
     5941 atactcgcgt ttgcttatcc cgacaacagc cttagccgct ggggagcgcc acgctcaacc
     6001 tgccagttat tacccaaagc gaaagcctgg ctggcgaaaa aaatgccgca gtggcggcgt
     6061 atattacaag gcgagacggg gtacaacgaa ccagacgtgt ttgcggtctg tcgtttagtc
     6121 tcaggtttcc cctataccga tcggcagcag aaacggctgt ttattcgtaa cttctttacg
     6181 cttcaggatc ggctggattt aacccatgag tatctgcacc tggcctttga tggttatccc
     6241 accgggcttg atgagaacta tatcgaaacg ctgacccgac aattattaat ggactaatgc
     6301 tatgcgaaag atttttcttc cgttgttact ggtggcactt tcgcctgtcg ctcacagtga
     6361 aggtgtgcag gaagtcgaga ttgacgcacc gctctctggc tggcatccgg cagagggcga
     6421 agatgccagt ttttcacaat cgatcaatta cccggcgtcg tccgtcaaca tggcagatga
     6481 tcaaaatatc tcagcgcaga tccgcggcaa aataaagaat tatgctgcgg cgggtaaagt
     6541 tcagcagggt cggctggtgg tcaacggtgc cagtatgccg cagagaattg aatccgatgg
     6601 ttcatttgca cgcccttata ttttcactga aggcagcaac agcgtgcagg tcatcagccc
     6661 ggatgggcaa agccggcaaa aaatgcagtt ttactcaacg ccgggtacag gaacgattcg
     6721 tgcacgttta cggctggttc tctcgtggga tacggacaat accgatctcg atctccatgt
     6781 tgtcacgccc gatggcgaac acgcctggta cggtaacacc gtgctgaaaa acagtggtgc
     6841 actggatatg gatgtcacga cggggtacgg acccgagatt ttcgccatgc cagcaccagt
     6901 tcacgggcgc tatcaggtgt atatcaacta ctatggtggg cgcagcgaaa cggaattgac
     6961 gaccgcccaa ctgacgctga tcaccgatga aggatcggtc aatgagaaac aggaaacatt
     7021 tattgtaccg atgcgtaatg ctggcgagct gacgctggtg aaaagttttg actggtgatc
     7081 catcccttca cacaacctgg ctctctcttt tttcagataa gactgctggg cgatctgccg
     7141 acgccaggat ataaccatta acaagcgtcg gcagattggt catttcttat ttttcgtact
     7201 gagaactgtc catctactcc ggctttcctg gttaaggcaa tggtttctaa gcgatacgca
     7261 ttattgcaat ggtgacagaa cattgcggta acacgctttt accgctacct taaccacact
     7321 ccatcggtca cctgaggcgg agcttcgccc ctttgaaata ccttgcttct tttcgtacaa
     7381 ccctgaaagc ctcgcgctac atgttcagag cattggcgtt agtgctctgg ctgttgattg
     7441 ctttttcatc cgttttttac atcgttaatg cgttacatca gcgagaatcg gaaattcgtc
     7501 aggaatttaa tctgagttcc gatcaggctc agcgctttat tcaacgcacc tctgatgtga
     7561 tgaaagagct gaagtacatc gccgaaaatc gcttatcggc agaaaacggt gtgctttccc
     7621 cgcgtggacg agaaacgcag gcggatgtgc ctgcgtttga accgctgttt gccgactccg
     7681 attgttccgc aatgagtaac acctggcgag gttctctgga gtcattggcg tggtttatgc
     7741 gctactggcg cgataatttt tctgcggctt acgatctcaa ccgggtattt ttaatcggca
     7801 gtgataacct ctgcatggcc aatttcggtc tgcgtgatat gccagtggaa cgcgataccg
     7861 cgttgaaagc gttgcatgaa cgcatcaata aatatcgaaa tgcaccacaa gatgatagcg
     7921 gcagtaacct ctactggatc agcgaaggtc cgcgccctgg cgtcgggtat ttttacgcgt
     7981 tgacgccagt ttatctggcg aaccggttgc aggcgctttt gggtgtcgag cagaccatcc
     8041 ggatggaaaa ctttttctta ccgggtacgt tgccgatggg ggttaccatt cttgatgaaa
     8101 atggtcatac cctgatttcg cttaccggac cagaaagtaa aattaagggc gatcctcgct
     8161 ggatgcagga acgctcctgg tttggctata cggaagggtt ccgggagctg gtgctgaaga
     8221 aaaatctgcc accctcatcg ctaagcatcg tgtattcggt gccggttgat aaggtgctgg
     8281 aacgcattcg catgttgatc cttaacgcaa ttttgctgaa tgtgcttgcc ggagctgcat
     8341 tgtttactct cgcacggatg tacgagcgac gtattttcat tccggcggaa agcgacgccc
     8401 tgcgactgga agaacatgag cagttcaatc gcaagattgt cgcctccgcg ccagtgggta
     8461 tctgcatttt gcgtaccgct gatggcgtca atattttaag taacgaactg gcgcatacct
     8521 atctcaatat gcttacgcat gaggaccgcc aacgactgac gcaaattatc tgtgggcagc
     8581 aggtcaattt tgttgatgtc ctgaccagca acaataccaa tctgcaaatc agcttcgtcc
     8641 attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct ggtggatgtt tcttcgcgcg
     8701 tgaagatgga agagtcgttg caggagatgg cacaagcagc ggaacaggcg agccagtcaa
     8761 aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac gccgctgtat ggcattatcg
     8821 gtaacctgga tctgttgcaa accaaagagt taccgaaagg cgtcgatcgg ctggtgacgg
     8881 caatgaacaa ctcttccagc ctgttgttga aaattatcag cgatattctc gatttctcga
     8941 agattgaatc ggaacagttg aagatcgaac cgcgtgagtt ttcaccgcgt gaagtgatga
     9001 accacatcac cgccaactat ttaccgctgg tggtacgcaa gcagttaggc ttgtactgct
     9061 ttattgaacc ggatgtgcca gtggccttaa atggcgaccc gatgcgttta cagcaggtca
     9121 tctccaacct gctgagtaac gccataaaat tcaccgatac cggctgtata gttttgcatg
     9181 ttcgcgctga tggcgattat ctctctatcc gtgttcgcga taccggcgtg ggcattccgg
     9241 cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt cggaacgggc gtacagcgta
     9301 acttccaggg gaccggtctg ggtctggcga tttgtgaaaa actgatcagc atgatggacg
     9361 gcgatatctc ggtagattca gaaccgggaa tgggcagcca gtttaccgtg cgtattccgt
     9421 tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg gttgagtggt aaacgctgct
     9481 ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga aaccagtttg cagcgcagcg
     9541 gcatcgtcgt tacaacatac gaagggcagg aaccgactcc cgaagatgtg ttaatcactg
     9601 acgaggtagt gagtaaaaaa tggcagggta gagcggtagt gaccttctgt cgtcgccata
     9661 ttggtattcc gctggagaaa gcgccagggg agtgggtaca cagtgtggct gctccgcatg
     9721 agctaccggc attgttggcg cgtatttatt tgatcgagat ggagagcgac gatcctgcta
     9781 acgctctgcc gtcgacggac aaagcggtca gcgataacga cgatatgatg attctggtcg
     9841 tggatgatca tccgattaac cgacgtctgc tggcagatca gttgggatcg ttgggctatc
     9901 aatgtaaaac cgcgaatgat ggcgtcgatg cgcttaatgt acttagcaag aatcatattg
     9961 atatcgtgct tagcgacgtc aacatgccaa atatggatgg ttaccgcttg acgcaacgca
    10021 ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac tgctaatgcg ttggctgaag
    10081 agaagcagcg gtgtctggag tccggtatgg acagctgcct gtcgaagccg gtaacgctgg
    10141 atgtgataaa acagacgctg acggtatatg ccgagagggt caggaaatcg cgggaatcgt
    10201 aggacggata aggcgtttac gccgcatccg gcattcagtg caaatgccag ataagacact
    10261 aacgcgtctt atctggccta caggtgatta gtctttatct gccggactta acgtcacaga
    10321 agagagataa ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt
    10381 cttctggcta ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag
    10441 gaagccttcc gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc
    10501 gtaaccacca gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt
    10561 ccctttctgc agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac
    10621 gatcccttcg atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatggt
    10681 cagaacaata atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc
    10741 atcgccgtac ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt
    10801 cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc
    10861 aatttgctca agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat
    10921 tacgttcata ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt
    10981 caatgtcgct gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca
    11041 gcgtttcaca taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac
    11101 gatgagccgt ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct
    11161 taacatcatc cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg
    11221 cattttcatc tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa
    11281 ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac
    11341 caggcccaat ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa
    11401 gattagacgg attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg
    11461 gtgtgataca ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg
    11521 ccgaagtaac atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa
    11581 cttccgggtc agcggcgagc attttgatat gcacagagta gcgtgtacca agcccatccc
    11641 gcgttttgat gtttaaatga ccgcccagtt tacgcgccag ttgatcgctc agccagaatg
    11701 ccagcgggtc cgccttgcca tagcgatcgt tttgggtctg gttgataaac gggaagtgca
    11761 aattatccat ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg aatgtcaggc
    11821 ggtcttcaga ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg
    11881 tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc
    11941 gcatatcgtg tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg
    12001 caggcaacac tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct
    12061 cacttttcca gctatcatcc gcaagcatgt tcgctaactg tatttcatca accagccgca
    12121 ccagcacatc tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc
    12181 tctccgccag ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca
    12241 tccggccctg ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca
    12301 gcacttcgcg atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc
    12361 ggaacatgcg gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct
    12421 gttccgccat ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt
    12481 tgttacttat gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca
    12541 gtgagactat ctcttcatta attgcccgta aaatgcgcaa ttcgttattg accgccgtgc
    12601 tgggtagact ttctgtactg cggctggaga aatggcggaa tgtggtatag ccaaataacg
    12661 ccagcgccag caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca
    12721 gcaataaggt gccataagga acctgccaga ccaagcgcat atcggtagag ttgagtgccg
    12781 aggagatttc aatcttcgta ctgttaaagt ggatactgac gctatccgtc ccttctttct
    12841 cattatcatt gtttcccgtt gccgtcgcgt ctggctcaag gcggaaactg tccagcggca
    12901 tacccggtgg gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc
    12961 ctggctggtt aaaggtagta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc
    13021 gcatgttaga aaagctttcg cgttcatcga gggcgttggc ctgttgcaac atctctgcac
    13081 gacgtgaatc aacaatgtcg ctgacggtcg attctttaaa tccggaggtg agatctttaa
    13141 ggggtagggt tgagatcagc accagactgt tatcctgacc attcaggtaa tacatcgacc
    13201 acggtacatt ttctgcgccc cacaatgtat ccagataagt ggacatccgc tgagtcatct
    13261 caagcgttga gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct
    13321 ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg
    13381 aggtcgtcgc ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc
    13441 gcttttggat agcgtgggta atgtcgacaa tctggtaact tttatctttc agccatgcgt
    13501 taacggcgct ttgtaccatt acacccatcg tcaccagtaa cacaatgatc aacagtaaaa
    13561 agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct
    13621 gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt
    13681 acagtgtgaa attccagact cttacctgat tgatgattct ctattctccc gtgatattta
    13741 tcatcctgca ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca
    13801 aaaagcccaa cgaaaataat tgcaaataaa caaagat
//
LOCUS       DAZFRJ010000121        13772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000121 DAZFRJ010000000
VERSION     DAZFRJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.122"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHKUN_003196"
     CDS             complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHKUN_003196"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO6661407.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(805..1353)
                     /gene="mepK"
                     /locus_tag="ACHKUN_003197"
     CDS             complement(805..1353)
                     /gene="mepK"
                     /locus_tag="ACHKUN_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO6661408.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHKUN_003198"
     CDS             complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHKUN_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6661409.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHKUN_003199"
     CDS             complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHKUN_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO6661410.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHKUN_003200"
     CDS             complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHKUN_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO6661411.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHKUN_003201"
     CDS             complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHKUN_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO6661412.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHKUN_003202"
     CDS             complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHKUN_003202"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO6661413.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11027..11806
                     /gene="elyC"
                     /locus_tag="ACHKUN_003203"
     CDS             11027..11806
                     /gene="elyC"
                     /locus_tag="ACHKUN_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO6661414.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11783..12676)
                     /gene="ycbJ"
                     /locus_tag="ACHKUN_003204"
     CDS             complement(11783..12676)
                     /gene="ycbJ"
                     /locus_tag="ACHKUN_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO6661415.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHKUN_003205"
     CDS             complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHKUN_003205"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6661416.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHKUN_003206"
     CDS             complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHKUN_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO6661417.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
BASE COUNT         3168 a         3840 c         3442 g         3322 t
ORIGIN      
        1 gtcccctgca atattttgaa tttgcacgat tttgtaggcc ggataaggcg ctcgtgccgc
       61 atccggcata aacaaagcgc actttgccag aaatccaggc ccgcattaaa agcgggcttt
      121 tttatgtgcc ttaccagacg ggcatttcgt cttgcaggaa gggattatgc aggcgttcat
      181 aaccaagtgt ggataatggt ccgtgacccg gaataaatgt cacgtcatcc cccagtggca
      241 gcaatttatc tttgattgaa gaaatcagtt gattatgatc gccacgcggg aagtcactgc
      301 gccctactcc gcctttgaaa ataacatcgc cagaaatcag cagctttgcc cgatcatcaa
      361 aaaacacgac atgacccggc gtatgcccag ggcaatgtaa cacctgtaaa gtcacattcc
      421 ctatgctgat ggtatcgcct tcgttcagcc aacgatctgg cgtcagcggc tggcactctt
      481 ccagaccaaa catacgactt tgcgcaggca agccttgcag ccagaactca tcttcttttt
      541 ccgggccaaa aaccggcacg ccgtaatgtt gcgccagttc cgccgctgcg ccaacgtggt
      601 ccagatgacc atgcgtcagc aggatctgca tcagtgtcag gccgctgtca tcaacttcct
      661 gtttgatttt ttccgcatcg ccgccaggat cgaccagtgc ggccagacgg gtttgttcac
      721 accagattaa tgaacagttc tgggagaatg cggtgaccgg aataatacga tagttcatac
      781 tgcccctgtt tcgttaagca attactacca gtgccgtgct ggcccggtat caatatgcac
      841 aaagttacta cgtggataat atcctacacc acctgcgcgc atagataacg cggctttgcg
      901 aatattgctt aacgcgatac cttcaatatg gaaatccatc gcctggcctt tagtgtgata
      961 gcttttcttc gctactccac ggctgcgggc gcgtagttca ttgttggtat caatagaacg
     1021 ataaccggaa atgagttgca ccggtttgcg cgtgcctaac aacccttgca ggcgatacaa
     1081 ctggtcgaat aatcctgggt cgatggactt tattttgttc gcgcggtaat cgcggaaaaa
     1141 atggttaagt tttgccaatt cttcctgaat atagcctctg ccatcgaaaa actccgcttt
     1201 gattgactct ccggtatgaa gattattgag tgtcaaaatg cgcgggcgtg gggtagagag
     1261 tgttgcaaac gcaggggtcg gcaggatggc ggcaccgagt gcaacgccac caagcgccag
     1321 caatttgcgg cgattagcgt cgaatttgtc catgataatc aagtctacag gtcaatgtta
     1381 tcgtttatat gcacttctgg cgcacaagag gcaccttaac cgcccgtaaa gcagacgtca
     1441 agatgactca accccagcag tgacgggggc tgcagagaat cgcccccaat catgctaatt
     1501 attacgacaa ctgatttccc cgaactactt catttacctg attaattgtt ccgctttcga
     1561 tacgatttgc gagctggatc gcgcaggcag atcataattg taaatatctg tacgatactg
     1621 ggtacgacca tctgcaccaa caaaggccgt caggtagtag agattcaccg gaatcgactg
     1681 ccgaatattg acgtaacgtg tatcaccctg cttcagcgca tcagaaatac gtttgtcatt
     1741 ccagcctgca tcctgcaaca gcatattcgc cagatcggaa gctttattca ctcgtacaca
     1801 gcctgagctc aatgcgcgtg tatcacgctt gaacagattg tggttcggcg tgtcatgcaa
     1861 ataaatggcc tctgaactcg gcatattgaa tttatagcgc cccagcgagt tccgtgggcc
     1921 tggagcctgc tggaagcgga acggtaaatt cgaggccgtg attgtagacc agtcaacctg
     1981 ccatgggtca atcgcttctc tgctgttcca gccgcgcatc accgtatagc catggctttc
     2041 gagatatccc ggatcgttgc gcacttttgg cagaatatct ttgcgtgcca gagttggagg
     2101 tacgttccac ggcgggttta ccactacgtt gttaagggca ctgctcatca tcggcgtttt
     2161 gcgatcgggg cgaccgacaa tgactcgcga atccagcacc tgattgccgt tctgatagta
     2221 gaccagcgaa taggccggaa tgttaaccat gatcccggta gaaagctctg ttggcagcaa
     2281 gcgcaatcgc tggatgttga gagccaacac gccagcacgc tgggcgggcg ttacgtttaa
     2341 ccagtcacgt gttgccgggc caatagcacc atctgccccc aatccttgcc atgcctgaaa
     2401 acgtttaacg gcttccacca gttcattatc gtaggcggca cgaacggcag gcgcaggttt
     2461 actacgagac gtcgtttgct tgtccatcgg cttagtttct gctgtttcaa cagtaacagc
     2521 ggatgggctg actaccgcgt cagttggcgt gtcatcgcca ggtagagtaa ttttcggccc
     2581 cccgtccaac atgcctgtgc gttgcaatat ttcgcgcaac gccggtacgt cgttactcca
     2641 ctgccctggg cgcaacgttg ctttgccggt cagttggggc cacggtttgg tgtcactgag
     2701 taaggccagt aacgattcat gcatcgccgc atattgcgga tgctgcggtg ccagtcctgc
     2761 aacaaacgta ggcaattgac ctttatccag cgccagctgc cattggttaa tcaccgagag
     2821 cggcggcgtt gcaagtgcat aaggtttact gctatatagc cagcgagtgc ctttgaccgg
     2881 aatatttgca atgaaatgga gatagcccat catcgcatca gagagcacta cgtcgcgtgc
     2941 catcccgtta acaccaggat cggtcagtaa ctctacccat ttattaaact gcggctggaa
     3001 accggcaatc gccacctctg ccagctgttg ctggaaggct ttaacagcat cacggttttc
     3061 ccacatgggt tgcatatcgc gtgcggcata caacagttga agctggttaa gataaaccgg
     3121 cttgtaacct gcgggtaatt gagattcgat ttgcgtgcgg gctttttctg ccgcaccttc
     3181 aggcaatggc tggatccccg ccattattgc cgtggcttgc gcctgcggca gtgcctcgcc
     3241 ctgttcactg acagccaccg ggctgtcgcc agggattact tcaggctcat cggcctgcgc
     3301 attgaacagt ggagcgaatg ttacggccag gcacaaactg attgccgaca gccgacgacc
     3361 acacatcata ttaagcaaca tcccttgccc cctgttttta tcatactctg aatagccagg
     3421 ctgttatttt acgacttgcc tgtacaacgc caatactcac gaaagtatga cgagcaagcc
     3481 tgttactcat caggcttatc ttcagtatat aaacgccgat acgtttttac ataacctaat
     3541 gtaaagaagt ttaaagaaaa gcgcgccgta atgtgccact tttttagtgc ggaaacggag
     3601 ttttcggaaa aagaaaaggc ggcattgctg ccgccttaat tttaactcgc ctgagaaggc
     3661 gcttcgtcac ttcctggaag cgtttcaggg agttgcggcg caaatcctcg caggccgacg
     3721 acgtgaacgt gttcggtatt ctggaagact ttacgcacca gtttataggt ggtgcctttc
     3781 tccgggctga tattttccgg cgctgcgatg atgagctgca tttgcaaacg ctcacacaat
     3841 tcaaacagcg tggcgataga acgagcatcc agtcgcgctg cttcatcgag gaacagcagg
     3901 cggcaaggag agatatcttt accgcgcagg cggcgagatt catcttccca gctttgtacc
     3961 accatcacca gaatcgacat accggtacca atcgcctcac cggtcgacaa tgcaccagac
     4021 tctgcgcgca accaaccatc ggaaccacgg ttaacctcaa cttccatttc cagatagttg
     4081 cggtaatcca gcagttcttc gccaatggtc tgcggcgtgc gctgtcccat atcaatctgc
     4141 ggattaagac gctgatacaa tttcgccaac gcttccgaga aggtcagacg gttgctgtta
     4201 aacagatcct gatgctgctc gtgctgttca gagagcacat ccagcagcat ggcgtgcgtt
     4261 tcacgcacgt tcacgttgag gcgcacgctg ttcacctgac caaacgatac gttctgcaac
     4321 ccctggttga gcatacggat acggttctgc tcgcgctgaa tggttttgcg aatgatgttc
     4381 gccacgctgc gggaactgat cgccagtttc tgttcacggg aggttaattc ttcggtcaga
     4441 cggctaagtt cgatctccat ctgttcgata gcttccaccg gatcatcggt acgaataata
     4501 tcctgacgaa tacgttcacg cagatgctga taaaccgcca cgaagaactg aattttacgc
     4561 tccggacgtt tcggatcttc cgacatgcgc agcacgtcgc gcagatgttc gttatccgcc
     4621 accgccagac gcagcgcacc caatgcctta tccgacatag agcgcaaatc atcagcggag
     4681 agataagcca gctcacgacg gtgtaagcgg cgctcaacgc cgttatcttt caccatgcgc
     4741 atcaccgcac accagcccgc tttggcggtc actacctgct cgcgcatctc aaagtaatcc
     4801 cgctccagct tgcgcagttt gcgggtcagg ttgtccatct ccgcttcgca gaaggtaagc
     4861 gctttttcca gttgattgcg gcgtgaacgg ttattgctca gttgcgcatg cagctcgtca
     4921 cggcgaatac gcgcccgctc ttctgccccg ctatcagcac gcacgccgat atcctgcaat
     4981 tcacgttgca gatcgttgag tagctctttt ttggtgtcgt aagaactttt cagcgaagcc
     5041 agcacctggt tgtactgact caactgcgct gcgtgaccac gcaacgcttc acgagcgcga
     5101 gtacgctccg cttccgcctg ttccagacgt tcacgcagct tttcgttgag atcgctatta
     5161 ccgctcagca tttctgccga gtcagaatag ctaaagtgcg cacgacgctg caccacttcc
     5221 gtcagggcaa acgcctgctg acgggcatcg cgctgcatct gctgagagta cgcgtaatct
     5281 tcttttagct gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat cggttccagt
     5341 ttcgccagtt gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc ttcatccaga
     5401 cgttcgcgga tttcatcgac gcgatccgcc aggctgtcat cagccaacag gttgagacgc
     5461 ggcagaatgc ggttcagcgc cgtaacgcct tctttcgcct gctcaaactg aatacgctgc
     5521 tgctggttat cattttcatg attactcaac gcccgctcca gttcgacgcg acggctgttc
     5581 agttgacgga tttctgcttc cgggtcagac tcaaacgcaa ccgccagatg actgccgata
     5641 aagcggctga acgcctgatg cagacgctga gttttctgta catcaaagga gagcgtggcg
     5701 aagcgttcgg aaagcacttc acgctcggca tggaggcttt caatacggct ttcacgcgca
     5761 gcacgaccaa acagcggcac ttccgggaaa cgtgaataac gccactgacg atcggcgatt
     5821 ttcaccacta ccgctttttc cagctcatca acgctgaaca cgctgtcatc gaatgactgc
     5881 ggatctcctt cgatcagata gagatcttcc gggcaatcgg tcaagccttc caggtgttca
     5941 gttatctgtg acagatctgg caccacgatg gcgtggcgtg acgggccata cagcgctgag
     6001 aagtacggcg catcttccag gctaacgtcg tcataaattt ctgacagcag cacaccacca
     6061 aaacgctccg ccagcgcgtt cagacgctga tcttcagagc cgccaggctg gcttaaacgt
     6121 tcgatctctt catcgacggc gtttttgcgc gcgcccactt catcgcgttc aacaatcgcc
     6181 tctcgctcac gctccagcaa ctgttgcaga tattcggtga cgtcctggct ggaggtaaac
     6241 tcttcgccgc actgttcgct caactggttg agactgtttt gcgctgccag ccaaaccggc
     6301 gcacgctgca tcaaactctg aatgcgagac tgcagctgtt cctgttcctg gcgcagtgcc
     6361 atgcgctctt cacgggcgtt agacacgcta tcggaaagag aggcaatgcg cgcttccagc
     6421 tcctgatgca gagcttccag ctcgtcaatg tcaaaattct tgccctgacg tttgcagaaa
     6481 tctgccagca gacgctcagc ttcttgctgt tcgcgcagac gctgttccag ttcgcttaag
     6541 cgcatccgca acggctgaac ctgctctgcc aggtgacgct gatcgacccc ttcgcgcaat
     6601 agttcgcgag cgacatccca cgcctcgtta cgcgccagcg ggccgttgat tgccaccacc
     6661 agctgataag cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat tttctgctca
     6721 agagagagca ttttttcagt cgcttccagc tctttcgcct ggaaggtttc cagccattcg
     6781 gcggcgctgt cggcggttaa gtccggcaga tggcacagtt ctttggcgcg attaagcgca
     6841 gcaatcgcct ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc ctgctggtag
     6901 ttagcaagct ggcttttcag ctcgtccact tccagttcgg cagcttccgc gcgagcctcg
     6961 ttctcttcct ggcgttcgat ggcttctgcc accacttcgt tttgctcttc cagacggatc
     7021 tgcagctcat cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg cagtgcggtt
     7081 tgcaccaggt tcaggtgatc gctggccgcc tgataatccg cttccagatc gccttcggca
     7141 ccgttgtgct ctgccagctc acgcgccata tcgacgtgtt tgtactgctc agccgccagt
     7201 tgctgacgcg aagtatgtaa ctcgcgacga aactccaggg ctttgtcgag atggacacgg
     7261 cgctcgttgg cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc gctgatcaga
     7321 tgcttaaaca ggtcacggtc cgactgggtg acacgaatcg cttccagcgt catgcggttt
     7381 tcacgcagcg ccgcttccat gtcctggaac gctttacgca cgccgctgtt ttccggcaac
     7441 aggtagtcgc gcagagaacg ggtaatggca ctggagatcc cgccatacag cgaagcttcg
     7501 atcagacgat agaatttgct acggtcagat gcggagcgca gacgacgcgc gatgatgccc
     7561 agatcgaaca tcagcgagtg gtaatcggta atggagttga actgtttaaa ctgcacgcct
     7621 tccatcgcct cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc ctggcgttcg
     7681 ttcagggttt cggtcaccag ctgtgtcggc tgcaccgaca tcggcagtcc ctgaatggca
     7741 aatggtttga tatccacttt acgatcgcgt ccggcaacct gttgcagacg cacaccgacc
     7801 accacgcgct ggtggcgcga gttaatggtg tcgagcatcg aataacagac acccgctttc
     7861 agcttaccgt gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc cgtagtgtta
     7921 cggaaatgca gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc catggtggtg
     7981 gatttacccg ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag gtcaaaagtt
     8041 cgggcaaaaa agccgttcca gttaatcagc gtcagtgagc gaaatttacc gcgttcaatc
     8101 attattcttc ctctccgcta tctggctgat tttcttcggt ttcatcgttg agttgcagat
     8161 gattttcaat cggcattgct tcgccatcac gaatcaggcg acgctgtgct tcacggggat
     8221 cgtcgccagc acgcacatcg gctccgaagc ggaacaccga ttcggtaatg cgaaacttgc
     8281 tgctgtcgtg gcccataaac cacaccatgc ctaaacgacg cagacggttg agcgaagaac
     8341 gtactttctc ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg ttgttcacca
     8401 gtttcagcag ttttgcttca tcggccaggg tgagcagttc gtcgtacagt tcctgctggg
     8461 tgaaaatccc ctcattcgcc agccgttccg ggctgagata gagataacag aggattttcc
     8521 cgaccatcat atccagttcc gacaagacgg aacgagggat cagcgtggtg gaacgtgggc
     8581 gtaaatagaa gaacccttct ggtgcgcgaa taagctcaac gttataacgc gcgtaaaact
     8641 cttccagata ttcctgaaaa tccatcagga atgcatgatt atccagttcg tcgaggccaa
     8701 tatggcggcc tgaacgtaag gcgctgtcca gcgccggaaa taacggattc gccagcgcct
     8761 gcgccagctt aaccggcatc acttgttcaa tatttgtcga tgacatgcgc ctgtaccttg
     8821 gctccgtaat cattaatcgg ctgccatttc gctggcagtc cggtgaaatc tgcttgcgct
     8881 acgccaagac gtaccgcctg atcaataaca atacgcgcaa cgtcaaagtg acgtgcacgc
     8941 ggatactgtg acagatattc gcgtaccacc agaccaagat ccagcggcac ttgtctggtt
     9001 ttgtacacgg caagttgttc ttcgatgatc gccgccagct gttcgcggat ctcgttaaac
     9061 tcttcgtatt ccagatcctc aggaagttcc ccagtcactt cttcatcgcg cagtgccatc
     9121 tcttcgtcac gcatatccag cagacgatcg gcattggcat aagttagcgc ccacggctca
     9181 tcaaaatagg tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt tttatccata
     9241 tcgatcgcgg tacgaataaa tttgtgtacg tggcggtcgt agccaatcca caagtcgatg
     9301 gattgctggc cccaactgat aatacgatcg agtttgctct gcagatcgaa caccagacga
     9361 tcgacgaaat gcagatcgtc atgggtcatc gtcgcatcct gaatgcgcaa cagattagcc
     9421 tgcaatttgt cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt cccggaagtt
     9481 tccgaaagca acaattcaca gctggaaatc gccgcccgcc agtctttgtt cagcaactgg
     9541 gcgatatcgt ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt caggtcgata
     9601 ctgtcgaaaa tttctgctac cgaatatttc agtggcgcat agacattacg gtgccagtga
     9661 aattcatcac cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc cgccacaatc
     9721 gacaactgca tagaaagacg cagcgtagaa aactcgcgct gacggatgta gtagtcagta
     9781 atgccgatgc cgagcggcgt cagacggtaa attgcgttcc cttccgcctg ctcgctggta
     9841 aagcggttca gcagacgttg acgcaccatg tcgttgatcg cgttattggc gcgcacgccg
     9901 atggtttcgc tggtttgctc aaacgcatca ctcacatggc ggaatgcatc caccagctcg
     9961 ccttcactca tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc cagcagaaaa
    10021 gagagtcggt ctaccggcag cgagatggag aagtcatttt ttctggccca ggcaaccagt
    10081 tcggggactg tctgggaaaa ttcactcata ctttatcctt gctctgcggt ttgcgcgcgg
    10141 tgacatgaat ataacgcccc agggtaatat acggttcctg acggcaataa cgcgtttcta
    10201 attcaagtaa tgcttcatag cagtcgcgct gctggtgctt ctcgcgcaga taatcatgaa
    10261 acacgcgaac gcctgtctta cccataattt gccaaccagc ttcttccagc cacagataaa
    10321 cctgcgccgg gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt ttcggcattc
    10381 ccgcctgcac gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa ccatgcgcat
    10441 tgtagaacat taacgacaac acgccgcctg gacgcaacac tgaccacagg gtctgcaata
    10501 cactgcgggg atcggccacc cactcgagca ccgcatggaa caatatcaga tcaacgggcg
    10561 tttccaaatg cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg ttgtcgctca
    10621 caccttttgc ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa agatcgcata
    10681 aaatgacctg atgcccacgc tcggccattt tgattgcggt ctgcccttct ccaccgccag
    10741 catccagcac acgcagtttt tgcgggccca tttccgccag cacgcgatcg agatcctgcc
    10801 acagaatagc ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg gaaaactttt
    10861 ccgcaatatc atcaaaattg cgatcctgca ttactttctc caccggttgg ctaagacgtt
    10921 ttctgataaa gacgctattt tgtcacagcc acggcgagaa tgaacccgtc tggtgtaata
    10981 tcgctggcga tcccttgcta tatggttaaa aaaggaacca gaaaggatgc tttttacact
    11041 gaaaaaagtg attggcaata tgttgctgcc acttccgctg atgttgttaa ttattggcgc
    11101 tggcctggcg ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt ttattagcat
    11161 tgggtggctg gcactcttgt tattgagttt gcaaccggta gccgaccgcc tgctgcgtcc
    11221 catcgaaagc acctatccga catggaataa ttcgcaaaaa gtggactata tcgtggtgct
    11281 cggagatggt tatacctgga acccgcagtg ggcaccgagc tctaatttaa tcaataacag
    11341 tctgccacgc ctgaatgaag gtattcgctt atggcgcgaa aatccgggat caaaactgat
    11401 cttcacggga ggcgtagcaa aaaccaatac ggtgagtaca gcggaagtag gtgccagagt
    11461 tgcgcaatcg ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc caaaagatac
    11521 cgaagaagaa gctgcggcag tgaaacaggc gattggtgac gcccctttcc tgctggtgac
    11581 ctccgcatca catctgccgc gggcgatgat cttttttcag caggaagggt taaatccgct
    11641 accagccccg gcgaatcagt tggccatcga ctcgccgctt aacccgtggg aacgggcaat
    11701 cccttctccg gtctggctga tgcatagcga tcgtgttggc tatgaaacgc ttggccgcat
    11761 ctggcagtgg ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt gatgccagat
    11821 cgaagttgcg ccgactaaat cgtccggtat taaccagttg cgcaacttca tcccataaca
    11881 catacaacca acgccgccag atgaacgact ccgccactgg cgcgcgttgc aggtaactcc
    11941 agagcaaatc ttccgccaga gaattatcca ttagtcggaa cagttcgtat tctcgcggtg
    12001 cccaaagcat tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc gaatctttca
    12061 gcatgctgcg taaacagaag ttaccgtgaa tcaacacaca attgtcgtta aagccttcaa
    12121 ataatgccgg gagacattcg cgagtgcgaa acaggatccg cttatcctgc atcgtcagac
    12181 cggtgttatt gaactgattg agcgtggtcc atagcacttc aacatgttgc aggtaccatg
    12241 agggccagaa attttcctga gtattgtcga ccgcgccgac gcaaccgcga ctgtcctgac
    12301 ggtgccaggc cagtaaggct tcaacgattt ggtctttgag ctgttcccag cgttctggtg
    12361 ttcgggctgg tgcctccacc gaaacaccac gcatccgctc cagtagcagg acatcagggc
    12421 cggggtgttc ttcatgcgtc atcacaccat aaatagtcgg catacggaca gtcccacttc
    12481 ttgccagcat ggtggttttc catgccagtt gtcgggcttt tccgggcgta ctaaagcttc
    12541 ttgccattaa cggcattggg tttccctggc tgtcatacaa agcccacaac gccgtatccg
    12601 ctttttcatt gacgcactca atacgactga gtttttcgcc cagtaaatgg cttaattcgg
    12661 cacgcagctg ttccattcag attaccctca taaaagctac tgctttcata atgagcgcca
    12721 atataaaaga tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga ggaaaatcgt
    12781 aacggtacga cactcctccc aaaattggct gaagtgtcgt gaagtgaaat tagcgcattt
    12841 cagcgcgaac gcgctcaaga tcttcagggg tatccacacc tgtgccagga acttcctgag
    12901 caacagcaac atggattttt tcgccgtacc acagaacacg aagctgctct aacatttcga
    12961 tgtgttctaa cggacttgcc tgccagttga cgtaacgacg gataaagcct gcacggtagc
    13021 cataaatacc aagatgacgc aggaagttat cgccaacggt ttcaaggcct tctgcaaaac
    13081 gatcacgatc ccaaggaatg gtggcgcgtg agaagtacag tgcataccct tcagcgtcga
    13141 gaaccacttt caccgcattc gggttaaacg cttcttccgc attgtggatt ggcaccgcca
    13201 gagtcgccat acccacctga cgctgagcga ggttatcagc aacctgacga atgattgtcg
    13261 cagggatcat cggttcatca ccctgcacat taacgatcac cgtgtcgtcg ctgaatgcgc
    13321 atttttcgac aacttccgcc agacgttctg ttcctgactg atgatcggcg cgcgtcatac
    13381 atacttcacc gccagcggct tcaacggcgc gggcaacatc ctcatgatcg gttgccacga
    13441 tgatgcgctc ggcacctgat tcacgcgcgc gttcaagaac atgaacaatc atgggtttgc
    13501 cgttaatatc aaccaatggt ttaccgggca gacgcgtgga cgcgtagcgc gcgggaataa
    13561 tgaccacaaa actcatgatt tactctcatc agcagtcagc acgcgggctt ccgtttccag
    13621 caacactgga atgccatcgc ggagcgggaa agccaggtta tccagtttgc aaataagctc
    13681 ttgtttttcc tggttatacc aaagttttcc gttgcaaacc gggcaggcaa tgatttcaag
    13741 caaacgatga tccatagttc ctccagatgg at
//
LOCUS       DAZFRJ010000122        13626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000122 DAZFRJ010000000
VERSION     DAZFRJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.123"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..329)
                     /gene="acpS"
                     /locus_tag="ACHKUN_003207"
     CDS             complement(<1..329)
                     /gene="acpS"
                     /locus_tag="ACHKUN_003207"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO6661418.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVT"
     gene            complement(329..1060)
                     /gene="pdxJ"
                     /locus_tag="ACHKUN_003208"
     CDS             complement(329..1060)
                     /gene="pdxJ"
                     /locus_tag="ACHKUN_003208"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO6661419.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1072..1800)
                     /gene="recO"
                     /locus_tag="ACHKUN_003209"
     CDS             complement(1072..1800)
                     /gene="recO"
                     /locus_tag="ACHKUN_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO6661420.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(1812..2717)
                     /gene="era"
                     /locus_tag="ACHKUN_003210"
     CDS             complement(1812..2717)
                     /gene="era"
                     /locus_tag="ACHKUN_003210"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO6661421.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(2714..3394)
                     /gene="rnc"
                     /locus_tag="ACHKUN_003211"
     CDS             complement(2714..3394)
                     /gene="rnc"
                     /locus_tag="ACHKUN_003211"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO6661422.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(3667..4641)
                     /gene="lepB"
                     /locus_tag="ACHKUN_003212"
     CDS             complement(3667..4641)
                     /gene="lepB"
                     /locus_tag="ACHKUN_003212"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO6661423.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(4657..6456)
                     /gene="lepA"
                     /locus_tag="ACHKUN_003213"
     CDS             complement(4657..6456)
                     /gene="lepA"
                     /locus_tag="ACHKUN_003213"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO6661424.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(6654..7133)
                     /gene="rseC"
                     /locus_tag="ACHKUN_003214"
     CDS             complement(6654..7133)
                     /gene="rseC"
                     /locus_tag="ACHKUN_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO6661425.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7130..8086)
                     /gene="rseB"
                     /locus_tag="ACHKUN_003215"
     CDS             complement(7130..8086)
                     /gene="rseB"
                     /locus_tag="ACHKUN_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO6661426.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8086..8736)
                     /gene="rseA"
                     /locus_tag="ACHKUN_003216"
     CDS             complement(8086..8736)
                     /gene="rseA"
                     /locus_tag="ACHKUN_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO6661427.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(8769..9344)
                     /gene="rpoE"
                     /locus_tag="ACHKUN_003217"
     CDS             complement(8769..9344)
                     /gene="rpoE"
                     /locus_tag="ACHKUN_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO6661428.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9341..9496)
                     /gene="rseD"
                     /locus_tag="ACHKUN_003218"
     CDS             complement(9341..9496)
                     /gene="rseD"
                     /locus_tag="ACHKUN_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO6661429.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            9752..11374
                     /gene="nadB"
                     /locus_tag="ACHKUN_003219"
     CDS             9752..11374
                     /gene="nadB"
                     /locus_tag="ACHKUN_003219"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO6661430.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11359..12096)
                     /gene="trmN"
                     /locus_tag="ACHKUN_003220"
     CDS             complement(11359..12096)
                     /gene="trmN"
                     /locus_tag="ACHKUN_003220"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO6661431.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12228..13562
                     /gene="srmB"
                     /locus_tag="ACHKUN_003221"
     CDS             12228..13562
                     /gene="srmB"
                     /locus_tag="ACHKUN_003221"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO6661432.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         3242 a         3690 c         3429 g         3265 t
ORIGIN      
        1 gttacatgca tatttacaac gccgagcttt tccgccagtt ttaatgcctc accccatagc
       61 cgtagccgtg gtttgccgag ctcatcattg aatacttcaa attgattaaa cgccagacca
      121 ttgcggatcc cggtgccaaa cgcttttgct gcggcttctt tcacagcaaa acgcttcgcc
      181 agaaaacgca ccggctggtg gtgcgttttc cagattgccc attcgttatc gcttaatacg
      241 cggcgtgcca ggcgctcacc ggatcgggcg atcactgctt ctatgcgagc gatctctacg
      301 atatccgtgc ctaaacctaa tattgccatt agccacgcgc ttccagcatc agacgcttca
      361 tttctgccac cgcatctttc agtccggtca tcactgcacg accaataatg gcatgaccga
      421 tattcagttc atgcatctca gggatggcgg caatagcttt cacgttgtga taggtcagac
      481 cgtgaccagc gttaactttc agaccgagac tagcggcaaa ggtcgcggct ttggcgatac
      541 gcgccagctc ttgcgcctgt tcggcgtcag ttttggcatc agcatagcaa ccggtgtgga
      601 tctcgataaa cggtgcgcca acctctgccg cagctttgat ctgctcttca tcggcgtcga
      661 taaagagaga aacctggatc ccggcatctg ccagacgttt gcaggcatcg cgcattttgt
      721 cacgctgccc tgcgacgtcc aggccgcctt cggttgttac ttcctgacgc ttttccggta
      781 ccagacagca aaaatgtggc ttcgtctcaa cggcgatcgc cagcatctct tcggtcaccg
      841 ccatctccag attcatgcgg gtatccagcg tctgacgcag gatgcgcaca tcacggtcag
      901 taatgtggcg gcgatcttca cgtaaatgca cggtaatgcc gtccgctccc gcctgctcag
      961 caataaacgc cgcctgcacc ggatccgggt aagcagtacc gcgtgcgtta cgcagcgttg
     1021 caatatggtc aatgttgacg cctaacagta attcagccat gacaatcctc atcattcata
     1081 atgtgttttc accgttcgct taggcataaa ctgccggaac agttccctgc tctttaatgg
     1141 tttaccgcca agatacggct taagcgccat gcgggtaaag cgtttcgcgg cgcgtagtgt
     1201 gtctgcgtca ggaaattccc gcgcgtttaa cgcttttaac tgccttccgg tgaacgtttt
     1261 attgtcgata acgacgcttg cgataaaccc tttttcttcg cgataacgat acgtcatggt
     1321 gtcatctacc ggctcgccgc tacccgcaca atgggtaaaa ttgacgccat agcccagatg
     1381 cccgagcagt gccagttcaa agcggcgcag cgcgggttct ggcgtaccag tggcacctgc
     1441 aagagactga atgcagtgca agtaatcgaa aaagagttca gagaagcgcg tctcgtattc
     1501 cagtacgcgg gagagaagtt cgttgatgta cagaccgctg taaagcgtga taccgcttaa
     1561 tggcagcgcc agcgagacag cttcagcact gcgcagcgtt ttgacttcgc cacgcccgcc
     1621 gaaacgtagc aagagagggg tgaaaggctg taatgcacct ttcagagtag agcgtttaga
     1681 gcgtgcgcct ttggcaacca gacgcacgcg acccgattcc tccgtgaaga cgtccagcat
     1741 caggctggtt tcgctccacg ggcgactatg caggacaaat gcgcgctgcc agccttccat
     1801 cggagtgact cttaaagatc gtcaacgtaa ccgagactgc gcagtgcgcg ttcgtcgtcg
     1861 gcccaaccgg atttcacttt tacccacagt tcaaggtgga caggcgcttc gaacatttcc
     1921 tgcatgtctt tacgcgcttc aatcccgatg gttttgatct tggccccttt gttgccaata
     1981 accatcttct tctgcccttc acgctcaacg agaatcaaac cattgatgtc ataaccaccg
     2041 cgttcgttag agacgaaacg ttcgatctcc acggtcacgg agtacggcag ttcagcgccg
     2101 aggaaacgca tcagtttttc gcggatgatt tcagacgcca taaaacgctg tgagcgatcg
     2161 gtgatgtaat cttccgggaa gtgatgggtc gcttcaggga gatgcttacg cacgattgct
     2221 gcgatagtgt cgacattcag cccggtttca gcagagatcg gcacgatatc gaggaagttc
     2281 atctgacttg ccaggaactg taagtgcggc agcagatcgg ctttttcctg cacgttatcc
     2341 actttgttca ccgcgaggat caccggcgct ttgccatcgc gcagtttgtt gagcaccatt
     2401 tcgtcgtccg gcgtccagcg ggtgccttca acgacaaaaa tcaccagctc aacgtcgcca
     2461 atagagctgc tcgccgcttt gttcatcagg cggttaatag cgcgtttttc ttccatatgc
     2521 aggccagggg tatcaacgta gatcgcctga tacgcgcctt cagtatggat ccccacaatg
     2581 cggtgacgag ttgtttgcgc tttgcgggag gtgatggaga tcttctgccc cagcagtttg
     2641 ttcaacaatg tggatttgcc aacgttcgga cgtccgacga tggcaataaa tccgcagtaa
     2701 cttttatcga tgctcattcc agctccagtt ttttcaacgc ctgttcggcg gcagcctgct
     2761 cagccttacg acggcttgaa cctgtgccaa ccaccggttc actcaggccg ctgacctggc
     2821 agtggatagt aaattcctga tcgtgcgctt cgccacgtac ctggactacc agataagtcg
     2881 gcagcggcag atggcgaccc tgcaaatatt cttgcaagcg cgttttcgga tctttttgtt
     2941 tatcgcctgg gctaatttcg tccaaacgag tttgatacca gttgaggatt aatttctcga
     3001 cggtttgaat atcactgtcg aggaatacac caccaattaa tgcttcgacg gtgtcggcga
     3061 gaattgactc acgacgaaat ccaccgcttt taagttcacc tggccctaaa cgtaagcact
     3121 cgcctaactc aaattcgcgc gccagttccg ccagcgtatt gccacggacc agcgtggcgc
     3181 gcatccggct catatcgcct tcatccacac gagggaaacg gtgataaagc gcattggcga
     3241 taacgtagct cagaatagag tcgcctaaaa attctaaacg ctcgttatgt ttgctgctgg
     3301 cactacgatg agttaatgcc tgctgcaaca gttcctgatg attaaaagtg tagcccagct
     3361 tccgttgaag ccgattaatt acgatggggt tcatgcgata ccaataaata aatgcgtcaa
     3421 caattcagca cacgaaacag acctgatata catggctctg ctaactgctt cgctgcagtt
     3481 tcagtttacc ggtatatggg gaccaacgct gtttcgtgtg ccgtggcaac ctggaggtgc
     3541 caaccttaaa cttcggggga atattctata cacaacgacg ggggatgtcg ttagccacgg
     3601 gagatttatc tcataaataa ttcacgttgt cgccataacg gcgacaacgt gaacgaagat
     3661 gggctattaa tggatgccgc caatgcgact taagcgcaca cctgtcggcc attcgccttc
     3721 ttgcttatcg aaactcatcc agatagccgt tgcccgaccg accagattcg cttcaggcac
     3781 aaaaccccag taacggctgt ccgcgctgtt gtcgcggttg tcgcccatca tgaagtattg
     3841 tcccggcgga acaatccagg ttgccagttg ttgccctggc tgctggtaat acatccccac
     3901 ctggtcctgc gcaatcggca ctgtcagaat tcggtgcgtc acatcaccca gtgtctcttt
     3961 acgctcggaa agacgaattc cattttcttt ggtttcgttt ttcggcactt caaagaatcc
     4021 gctggtcgct tccccaccat tacggcgtga gaaggtctga acgaaatcgc tcggttccac
     4081 gttggagtag gtgaccggta gcgcgttttc acacgcctgg ccggaactgc atcccggttg
     4141 aatcgtcagc tcttttgaga ccggatcgta agtgacttta tcgcccggta aacccaccgc
     4201 gcgcttgatg taatcaagct ttggatcttc cggatattta aagaccacga tatcgccgcg
     4261 tttcggatga ccggtttcga tcagcgtttt ctggtagata ggatctttaa tgccataagc
     4321 aaacttctct accagaataa aatcaccgat taacagagtc ggcatcatcg aacctgacgg
     4381 gatctggaac ggttcataaa taaacgaacg tacaatcaat acgatagcca gcaccggaaa
     4441 aacagaagca ccggtttcca gccagccagg cttcggcgca acctttttca gggttgcttt
     4501 atccagtgag tcaccggcag ccgcctgcgc cgctgcctga cgttcccgcc gtttaggtgc
     4561 gaaaaagaat ttatccacgc accataaaat gcccgtcacc agtgtggcaa tcaccagaat
     4621 cagggcaaac atattcgcca tgccaactcc taagggttat ttgttgtctt tgccaacgtg
     4681 cagaatggcg aggaacgctt cctgcggcag ctcgacgtta ccgatctgct tcatgcgttt
     4741 cttaccttct ttctgcttct gcagcagctt tttcttacgg ctgatatcgc cgccataaca
     4801 tttagccagt acgtttttac gtagctgttt cacggtggat cgcgcaataa tgtgcgtacc
     4861 aatcgctgcc tgaatggcga tatcaaactg ctggcgtggg atcagatctt tcatcttctc
     4921 caccaactcg cgaccgcggt tttgcgaatt atcacggtgg gtgatcagcg ccaacgcatc
     4981 aacacgttca ccgttgatta atacgtctac acgtaccatg tcggacgcct ggaagcgttt
     5041 gaagttgtaa tccagagacg cataaccacg cgaggtagat ttcaggcgat cgaagaagtc
     5101 gagcaccact tccgccatcg ggatctcgta cgtcagcgcc acctgattac cgtggtaaac
     5161 catattggtc tgcacgccgc gtttttctac gcacagcgta ataacgttgc cgagatatgc
     5221 ctgcggcagc agcatgtgac attcggcaat cggctcgcgc agttcgtaga tgttatttac
     5281 cgctggcagc ttggatggac tgtcgacgta aataacttct cttgacgtgg tttcaacttc
     5341 atacactacg gtcggcgcag tggtgatcag atccagatcg tattcacgtt ccagacgttc
     5401 ctggatgatc tccatgtgca gcaggccgag gaagccgcag cggaaaccaa agcccagcgc
     5461 gctggagctt tccggctcat agaacagtga agcatcgttc aggctgagtt tacccagcgc
     5521 gtcacggaag gcttcatagt cgtcggaact taccgggaac agaccggcgt atacctgcgg
     5581 tttgactttc ttaaagccag gcagcgcctt ttctgccgga ttacgcgcca gcgttaaggt
     5641 atcgccgact ggagcgccgt ggatatcttt aatcgcacat accagccagc ctacttcgcc
     5701 acatttcagt tcagtgcggt caacctgttt cggcgtgaag atgcccagac ggtcggcgtt
     5761 ataggtctgc ccggtactca tgactttcac tttgtcgccc ttacgcaggg tgccgttttt
     5821 aatacggata agtgaaacaa cgcccaggta gttgtcgaac catgagtcga taattagtgc
     5881 ctgcaacggg ccttccggat cgccttccgg cggcggaatg tcgcgcacca gacgttcgag
     5941 aacgtcctgc acgccaacgc cggttttcgc tgaacagcgc accgcgtcgg tggcgtcgat
     6001 gccgacgata tcttcaattt cttccgccac gcgttcagga tcggctgccg gcaggtcaat
     6061 cttgttcagt accggcacca cttcgagatc catttccatg gcggtgtagc agtttgccag
     6121 ggtttgcgct tctacgccct gcccggcgtc gaccaccagc aatgcacctt cacaggcagc
     6181 cagcgaacgg gaaacttcat aggagaagtc aacgtggccc ggggtgtcga taaagttcag
     6241 ctgataggtt tcgccgtcag acgctttgta gtccagcgtc acgctttgcg ctttgatggt
     6301 tatgccacgc tcacgctcaa gatccatgga atcgagaacc tgcgcctcca tttcacggtc
     6361 agacaggcca ccgcagatct ggataatacg gtcagacagc gtcgatttac cgtggtcaat
     6421 gtgagctatg atcgaaaagt tacgtatatt cttcataaag tatgattatt gtgccttaat
     6481 gcccggttaa ccaggctttt agaagtcgct gttctgagct taacgtctgt attaatagaa
     6541 acgccgcatt ctacactaca acattgaggc gaggaaatgt tcataccgta tggattgtgg
     6601 tatctggaaa cgtcctcgca tttgttatgc aaaatgcaac aaagccagtg aaatcactgg
     6661 ctcgcgtctt ccgaagatgt ttcaaatcgc acaaggccag gcggcaatgc cacgcttaag
     6721 atgatcggtt gccattctgc ccgggcagca aacttacgcg agtagccgcg cgcaatcagg
     6781 aacccgccaa taccgccgag gaccgcacca cataatgctg cgacgtcaga agcaaagagt
     6841 agctgaaata gcgaagcgat gaggaataat cccaccagcg gcgacatata aaccagtaat
     6901 gcggagctaa gcaggctgcc ttcggcgatc cccaattcca ctttttgccc cggcactaac
     6961 ggctcatcac agggtacgac aatggtatgc gtggtttgcg ggccaagttt atttaacacg
     7021 cggctaccgc aaccggcgcg tgaagcgcag ctgctgcatg aaactttaac atcacaactg
     7081 accagcgcct gcccgttttg ccaggagacg acggtagccc actctttgat cattgcgctg
     7141 ccccgaactt aatattctcg gcaatgcgtt tcgccgtttg cggcggcagt tcaccgacaa
     7201 tggtgatttc ggcgttatca cgtacgcttg tactgacggt tctgcgtccg gtgcgcaaca
     7261 tctgatcggt gctcgatggc gtagcgcggt taacgtttac cgagaagctg aataatccgt
     7321 cggaatagag acgtgattcg ataggcatgt tgtccatcgt cggtagcgga cgtcgactac
     7381 tggaaacttc gctaaaaccc tgtggcaacc aggttggcgt ccagctgaat ttagcttttt
     7441 cacctacagg aacagaaagc aacggcggca aatttgcctt cgccagcgtc tgcatactgc
     7501 tactgatatc ctgattgacg ttaaaagcaa tcacgcgaaa ttgttccagc gtttcaccat
     7561 cgcgatcaag gagatcaacc ctcatcggta atttcgattc gatgtccatc cacacgatgt
     7621 agctgtagcg tgtaccatct cgggcaacca cgcgaatgac ttcgcaaaga cgatcagcaa
     7681 tacgcgtgcg cccgacggag ataaagtcgt agtaaggaga aaggcgtttg aaatcggtat
     7741 agatgagcga tggcagagaa tcaacaatgt aatcgccatt aagcgtgaac ggttcaagtc
     7801 ccggttcaaa atagctgatt tcattgccac gctgtaccac ttcccggcgc gggccatcca
     7861 tttgcaacaa ttgtgcaaga ggacggttat cgaggcgtgc atgtcgataa cgcagagact
     7921 caacaccctg tttattgatg ctgatgaatg acagctcgta attcagtgac tgactggcca
     7981 ggttcatctg ctgtaataac gccccggacg cgggagtggc cgaggcgtta gcagagaata
     8041 acaggctacc tgtcactaat gacatggcaa accaaagttg cttcattact gcgattgcgt
     8101 tcctaaagtt tgaattcctg gcacctgtac agcggcttgc tgggtttgcg cctgctcaaa
     8161 ctgaagctgt tcagagtgga gtcggcgttg cagttcgtaa tcctgcaaca ttgcattaat
     8221 gcgacgacgc tgctcctgta cctgctgctg ttgaccattg tttgcggtcg cttcagaagg
     8281 tactcccagg cttaccgggc tggctttacc catcatcggc agtgtattaa ataccggcgt
     8341 ttcgggctgc tgggacgttt cagattgtcc attatagtgc tggacgccaa cgataactgc
     8401 aagcgatacg catgctgcta cgcccatttg ggtaagctgt gccgcccacg gacgtacttt
     8461 ctgccagaat ggcattttct gccattgatg cggcgcaggc tgggcttccg ggatcaatgt
     8521 cgccggttga cgtactggct cttcttcaat ggcggccatc acgcgtgaag agatatcgaa
     8581 atggagcacc tcgggagtat caccccgcat tgagtcacgg attaagtgat agctttccca
     8641 ggttttctgc atttctgggt tatgagccag ttcgttaagc agctcactat ccagcgtttc
     8701 gccatccatt aaagcggaaa gttgttcttt ctgcatgcct aataccctta tccagtatcc
     8761 cgctatcgtc aacgcctgat aagcggttga actttgttat caatagcttc cctcgctcgg
     8821 aagatacgtg aacgcaccgt acctaccgga caatccatga tagcggctat ctcttcatag
     8881 ctcaggccat ccagctcccg caaggttatt gccatgcgca aatcttccgg gagggactca
     8941 atagttcgga aaactatctg tctcagttct tctgacaaca ttaagttctc agggttcgaa
     9001 atttctttca acgcgccgcc actttcgaag ttttcagctt caatggcatc cacatcactg
     9061 gaaggtggac gacgcccctg agcaaccagg taatttttcg ctgtatttac agcaatccga
     9121 tacagccatg tataaaaagc gctatctccc cggaacgaat ccagcgcacg ataggcttta
     9181 ataaaagctt cttgtaccac atcgggaaca tcacccgacg gcacatagcg ggaaaccaga
     9241 ctcgccactt tatgctgata gcgcactacc agtaggttaa aggctttctg atctcccttc
     9301 tggacccgtt caaccaggac ctggtccgtt aactgctcgc tcatccgagg taaagtctcc
     9361 ccaaaccaaa tttccacgcg ctatcgaaac gccactccat tagctgcaat ttgagcaagc
     9421 aaagggttag agtgtctcgt ttttgtaaag ttccgtaacg catctgtttt tgtttgtcat
     9481 gctgtagacg gatcattatc tatcattata agtctacaga atctgaacat cgcattatct
     9541 gtgtagaaat gcccatttaa ctgcctgaag agtaacccaa cggccttttt atttcaccac
     9601 ctaatcctcc accagccagt aacttctctt tttctcgccg ccctgcgtca gcgtgtttag
     9661 ccactgtaac aaatattaaa atagcaggtg tttattcgca caacatgatg ctatgctgac
     9721 caaacggtgt ttagtaaatt aaacaaagaa aatgaatact ctcccagaac attcatgtga
     9781 cgtgttgatt atcggtagcg gcgcagccgg actttcactg gcgctacgcc tggctgacca
     9841 gcatcaggtc atcgttctaa gtaaaggccc ggtaacggaa ggttcaacat tttatgccca
     9901 gggcggtatt gccgccgtgt ttgatgaaac tgacagcatt gactcgcatg tggaagacac
     9961 attgattgcc ggggctggta tttgcgatcg ccatgcagtt gaatttgtcg ccagcaatgc
    10021 acgatcctgt gtgcaatggt taattgacca aggggttttg tttgataccc acgttcaacc
    10081 gaatggcgaa gaaagctacc atctgacccg tgaaggtgga catagtcacc gtcgtattct
    10141 tcatgccgcc gacgccaccg gtagagaagt acaatccacg ctggtgagca aggcgcagaa
    10201 ccatccgaat attcgtgtgc tggagcgcag caacgcagtt gatctgattg tttctgacaa
    10261 aattggcctc ccgggcacgc gacgggttgt tggcgcgtgg gtatggaacc gaaataaaga
    10321 aacggtggaa acctgccacg caaaagcagt ggtactggca accggcggtg cgtcgaaggt
    10381 ttatcagtac accaccaatc cggatatttc ttctggcgat ggcattgcta tggcgtggcg
    10441 cgcaggctgt cgggttgcca atctcgaatt taatcagttc caccctaccg cactgtatca
    10501 cccacaggca cgcaatttcc tgttaacgga agcactgcgc ggcgaaggcg cttatctcaa
    10561 gcgcccggat ggcacgcgtt ttatgcccga ttttgatgag cgcggcgaac tggccccgcg
    10621 cgatattgtc gcccgcgcca ttgaccatga aatgaaacgc ctcggcgcag attgtatgtt
    10681 cctcgacatc agccataagc ccgccgattt tattcgccag catttcccga tgatttatga
    10741 aaaattgctc gggctgggga ttgatctcac acaagaaccg gtaccgattg tgcctgctgc
    10801 acattatacc tgcggtggtg taatggttga tgatcatggg cgtacggacg tcgagggctt
    10861 gtatgccatt ggcgaggtga gttataccgg cttacacggc gctaaccgca tggcctcgaa
    10921 ttcattgctg gagtgtctgg tctatggctg gtcggcggca gaagatatca ccagacgtat
    10981 gcctgatgcc cacggcgtca gtacgttacc gccgtgggat gaaagccgcg ttgagaaccc
    11041 tgacgaacgg gtagtaattc agcataactg gcacgagcta cggctgttta tgtgggatta
    11101 cgttggcatt gtgcgcacaa cgaagcgcct ggaacgcgcc ctgcggcgga taaccatgct
    11161 ccaacaagaa atagacgaat attacgccca tttccgcgtc tcaaataatt tgctggagct
    11221 gcgtaatctg gtacaggttg ccgagttgat tgttcgctgc gcgatgatgc gcaaagaaag
    11281 ccgggggctg cattttacgc tggattaccc ggaactgctc acgcattccg gtccatcgat
    11341 cctttccccc ggcaatcatt acataaacag ataaaaagcc tgggtcagcg ccgtatacgc
    11401 ttcggaatag ttctggtctg gcccacgaat gactaagcga tcgctaaagc attctcccgc
    11461 ctgcggggag aatgccagca gcacccgatg cggcagtcgc gcttcgtttt ccgccacatc
    11521 cgtccgcaaa cgtaaatgcc agcccatgct taatgccagt tcagtaaaac cattaccaat
    11581 ctgctctggc agcaccacgc agaaaaatcc ctcttcggta atgcactccg ccgcacaggt
    11641 cagcagcgat gggtgatcaa gcgtagtggt atagcgagcc tgttcccgtt gaggtgtcgc
    11701 gcactctact ccctgctgat agtaaggtgg gttactgatg attaaatcga agcgtactgt
    11761 ctgctgtgtg atccactgct gaatatccgc cgtatggacg ttaatccgct ctgcccacgg
    11821 ggactggttg atattttcct gcgcctgcgc ggcagcttca ctttccagct caacggcatc
    11881 aattattacg ctgtcactgg ttcgctgcgc cagcatcagt gccagcaacc cgctacccgc
    11941 gccgatatca aggcaacgtt tcaccccagc caccggtgcc catgcgccca gcaaaatgcc
    12001 atccgttccc actttcatcg cacagcgatc gtgagcaaca aaaaactgtt taaaagtaaa
    12061 tccattacga cgaagcacgg atgtagactg tgacatgaaa ataaaacctt gcaggaaaaa
    12121 cggcgatagc accgggtgag aacaataccc gagaagcgat atccatacaa acagatgaag
    12181 attgcagccg taacgtctat aatcagcgcc ccacacagag gtagaacatg actgtaacga
    12241 ctttttccga acttgaactc gacgaaagcc tgctggaagc cctccaggat aaaggtttca
    12301 ctcgcccgac cgccattcag gctgccgcca ttccgcctgc gctcgatggc cgtgatgtac
    12361 tcggttctgc gccgacaggc accggtaaaa cggcggcgta tctgctgcca gcgttgcagc
    12421 acctgctcga tttcccgcgt aagaaatccg gtccgccgcg tattttgatc ctcaccccaa
    12481 ctcgcgagct ggcgatgcag gtggccgatc atgcccgcga actggcgaaa catacgcatc
    12541 tggatatcgc caccatcacc ggcggcgtag cctatatgaa ccacgcggaa gtgttcagcg
    12601 aaaatcagga catcgtggtc gccacgaccg gacgtctgct gcaatacata aaagaagaga
    12661 acttcgattg ccgcgcggtt gaaacgctga tcctcgacga agcagaccgt atgctggata
    12721 tgggcttcgc tcaggatatc gaacatattg ctggcgaaac gcgctggcgt aaacagaccc
    12781 tgctcttttc ggcaacgctg gaaggcgatg cgattcagga ctttgccgag cgtctgctgg
    12841 aagatccggt ggaggtttct gccaatccgt ccacccgtga gcgcaaaaaa attcatcagt
    12901 ggtattaccg cgccgatgac ctggaacata aaaccgcatt gctggtgcat ctgttaaaac
    12961 agccggaagc gacccgctca attgtgtttg tgcgtaagcg tgaacgtgtg catgagctgg
    13021 caaactggct gcgcgaagcg ggcatcaaca actgctatct cgaaggtgag atggtacaag
    13081 gcaagcgtaa cgaagcgatc aagcgtttga ccgaaggtcg cgtaaacgta ctggttgcaa
    13141 ccgatgttgc cgcgcgcggt atcgacattc ctgacgtcag ccacgtcttt aacttcgata
    13201 tgccgcgcag tggcgatact tatttgcacc gtatcggacg taccgcgcgc gccggtcgta
    13261 aaggcaccgc aatttcgctg gtggaagccc atgaccattt gctgctgggc aaggttggcc
    13321 gttacattga agagccgatt aaagctcgcg ttattgatga gttacgcccg aaaacgcgtg
    13381 cgccaagcga gaagcagacc ggcaagccat cgaagaaagt actggcaaaa cgcgccgaga
    13441 agaaaaaagc taaagagaaa gagaaaccgc gtgtgaaaaa acgccatcgc gacaccaaaa
    13501 atattggtaa gcgccgtaaa ccaagcggaa cgggcgtgcc accgcaaacg acagaagagt
    13561 aatctcaatg ccgggtttaa gcctggtatt aaaagtgctg gcaaacgcaa aactgcctga
    13621 tgcgct
//
LOCUS       DAZFRJ010000123        13437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000123 DAZFRJ010000000
VERSION     DAZFRJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.124"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..479
                     /locus_tag="ACHKUN_003222"
     CDS             <1..479
                     /locus_tag="ACHKUN_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO6661433.1"
                     /translation="DLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTN
                     TFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGI
                     SILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(633..2282)
                     /gene="yjcE"
                     /locus_tag="ACHKUN_003223"
     CDS             complement(633..2282)
                     /gene="yjcE"
                     /locus_tag="ACHKUN_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6661434.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2434..3783)
                     /gene="ghxP"
                     /locus_tag="ACHKUN_003224"
                     /gene_synonym="yjcD"
     CDS             complement(2434..3783)
                     /gene="ghxP"
                     /locus_tag="ACHKUN_003224"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO6661435.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4329..4793)
                     /gene="soxR"
                     /locus_tag="ACHKUN_003225"
     CDS             complement(4329..4793)
                     /gene="soxR"
                     /locus_tag="ACHKUN_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO6661436.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4879..5202
                     /gene="soxS"
                     /locus_tag="ACHKUN_003226"
     CDS             4879..5202
                     /gene="soxS"
                     /locus_tag="ACHKUN_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO6661437.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5205..6791)
                     /gene="pdeC"
                     /locus_tag="ACHKUN_003227"
     CDS             complement(5205..6791)
                     /gene="pdeC"
                     /locus_tag="ACHKUN_003227"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO6661438.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7221..7502
                     /gene="yjcB"
                     /locus_tag="ACHKUN_003228"
     CDS             7221..7502
                     /gene="yjcB"
                     /locus_tag="ACHKUN_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO6661439.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(7601..8137)
                     /gene="ssb1"
                     /locus_tag="ACHKUN_003229"
     CDS             complement(7601..8137)
                     /gene="ssb1"
                     /locus_tag="ACHKUN_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO6661440.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8391..11213
                     /gene="uvrA"
                     /locus_tag="ACHKUN_003230"
     CDS             8391..11213
                     /gene="uvrA"
                     /locus_tag="ACHKUN_003230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO6661441.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11248..11604)
                     /gene="yjbR"
                     /locus_tag="ACHKUN_003231"
     CDS             complement(11248..11604)
                     /gene="yjbR"
                     /locus_tag="ACHKUN_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6661442.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11608..12024)
                     /gene="yjbQ"
                     /locus_tag="ACHKUN_003232"
     CDS             complement(11608..12024)
                     /gene="yjbQ"
                     /locus_tag="ACHKUN_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO6661443.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12135..12848)
                     /gene="aphA"
                     /locus_tag="ACHKUN_003233"
     CDS             complement(12135..12848)
                     /gene="aphA"
                     /locus_tag="ACHKUN_003233"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO6661444.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            13250..>13437
                     /locus_tag="ACHKUN_003234"
     CDS             13250..>13437
                     /locus_tag="ACHKUN_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661445.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSSS"
BASE COUNT         3515 a         3488 c         3258 g         3176 t
ORIGIN      
        1 ccgaccttag tttctctaaa ctcgatttaa ttaactttga acatgctgat ctgtctcgcg
       61 tcaatctgaa caaagcaatc ctccagaata taaacttaat tgatagcaaa ctcttttgta
      121 cgtggctaac aaatacattc ctcgaaatgg ttatatgtac cggctctaat atggctaatg
      181 ttaattttaa taatgccaat ttaagcaact gccatttcaa ctgttctatt ttaacaaaag
      241 cctgtatgtt taatacccgt ctctatcggg ttaattttga tgaggctagc gtccagggaa
      301 tgggcatttc tattctccgt ggggaggaaa atatccccat tgatagtgat accctggtaa
      361 cactacagaa attctttgaa gaagattgta cctctcatac tggcatgtca caaactgagg
      421 ataatattaa tgcagtcgct atgaagatta ctgcagatat tatgcaacac gcagattgag
      481 gttaagtcga agaacagcca atgaattgac tttacaagag agataactta tttgttttag
      541 aatcatgaat gttagcccga gccaacctgt taaaggtgca aaagattgca cctttttcag
      601 attacctcgg gcctggttac gtcgctttcg gcttactgat tttcctcaat tagcaacgct
      661 tcaagcaaat cgagatcgtg cagtaatttt tgcagcgttt cgttgctgat ctcccgcgtg
      721 gcgcgcaggt ggtacagttc agcacgttca gaacgcaatg ccgccagacg gaagcgacgc
      781 tcaaggttct cttcctgcac ggaactttca acgtcgttac gtccatcggc gcgacgacgc
      841 aggttaccaa tgacgcgaga actgacctcc gtaagcagct ggttatcgat attctcttcg
      901 gtatccgccg ccagacgctc ctccattttc tggatcgcca caatcgccac ttctgccgtt
      961 gccgctcgcg caatacgttc ttctttcaat tgttgcgaat ggtccgcgac ttcaatgtgt
     1021 tgtagcagaa taggcaacat caccacgccg acaaacagcg agaagagaat gacgccagcc
     1081 gccaggaaca ccagctcata gcgcgccggg aagacgttac catccggcaa gagcagcggg
     1141 atagagagca caccggccag agtgatcgcc ccacgcaccc cggcgaaaga cgcgatcagg
     1201 atttctcgtg tggtccacga accaaactcc atcggcttct tcttcaggaa gcggttgctg
     1261 aactttttca tcgtccacag ccagccgaaa cggaccagca tcagcgccgc atatatcaga
     1321 ataatatcgg taaacagcat ccagatttcg acgttagggt cgatttctgc cgccatcagc
     1381 gacgtctcca gaatacccgg cagctgcaga cctaacagca ggaacaccat gccgttaaag
     1441 acaaattcca gcatcgccca ggtgctgttt gcgcgcaggc gcattgccag cggcgcacgg
     1501 cgcatcacac cggagcgggt gatggtcatc ccggcggcaa ccgcagcgag gatgccggaa
     1561 acgccaatat gttcggcaat cagataagaa gcgaatggca gcaagaacag cagaacgatc
     1621 tgcgtcgcgg gttcatcacc gccccagcgg ctgaggaatc gcagcgagcg accatacagc
     1681 cagctcacca caaagccggc gagaataccg ccaatggcta ctttcataaa ttcgaccgtc
     1741 gcaccgccaa cggtgaagat catcgtcccc atcgccactg ccacggcaaa cttcaacgac
     1801 accaggccgg aggcgtcatt catcaacgct tcgccctgca aaatacccat gatttttttc
     1861 gggatgcgcc cttcgccaac aatcccggag agcgccacag catcggtcgg agaaagcact
     1921 gccgccagcg caaaggcggg gatcagcgga atgcccggca ccacccagta aataaggaag
     1981 ccaatgccga ccacggtgac caccaccagc gccagcgcga ggccaaaaat ctctcgacca
     2041 tgttcgagaa attcacgggt cggcgttttc cagccatcag cgaacagcaa cggcgggata
     2101 aacaagacta aaaagagttc aggatcaaac tccacatgca aaccaaacgt cggccacgcc
     2161 agtagcgcac cgatggcgat ttgcataagc ggaagcggga tctgaaaggg catgacacga
     2221 gtgaccaccc cggacagcga gaccacgagg gtcattatca gtatggtgaa gaagatttcc
     2281 atgcgttccc tgtttgcgat gtttttttat gatctgagaa agacgttatg cctgaattct
     2341 cattttttca gaatatcgca aaagctcaca caatgtgcgg agaaaataaa aacgggcagc
     2401 cattgccacc cgttttcaga atccgatgaa agattagata gcccagccac ccgcatagaa
     2461 ggtcaccagc gccacggcga taacgaccgt accgatgttc aacttgcgcc attcgccgga
     2521 aaccagacga ccaatcacca gagtcgcgaa gccgatcatg atgcctgtta cgatgttaca
     2581 ggtcagcacg atgaataccg ccgtaaccag acccgccatc gcatcaacaa aatcagcaaa
     2641 gtcgattttc gccacgttgc tcagcatcag caggccaacg tacatcagcg ccggagccgt
     2701 tgcatacccc ggaacgaggt aagagagcgg agagaggaac agaatcagca ggaacagcac
     2761 gccaacggtg atagccgtca aaccggtttt accgcccgcc gccgtacccg ccgcagactc
     2821 gatgtatacc gctgccggag cagcacccac caggccagag aaaacgctgc tcatggagtc
     2881 agtggtcagt gctttcccac cgtcgatgat ctgcccatct ttatccagca ggttcgcctg
     2941 gccggcgacg gcacggatag ttccggtggc atcaaatact gccgtcatca ccagcgccag
     3001 aacgcttggc aggactacag gattcagcgc gcccataatg tccaggctgc caatcagtga
     3061 attgccgttt tcatcgctca atgaaggcat ggcgaaaacg ccggagaaat ggacgttagg
     3121 atcgaagatc aaaccgacaa ttgagatacc gataatggtc agcagaatgc caccagggac
     3181 tttcagtttt tccaggccga tgatcaccgc cagacctact agtgacataa tcaccgggaa
     3241 ggtcgcgaaa tcacccagcg caacgggcag accatcaagc gggtttttaa tcaccagacc
     3301 gacaccatta gcggcaatga gcagcaggaa cagaccgata ccgatccccg tgccgtgcgc
     3361 cacaccgtgc ggcaagttgc gcaaaatcca gctacggata cccgtggcag aaattaccgt
     3421 aaacagcacc cccatcagga acacggcacc cagcgcgaca ggtacgctaa tatgttgccc
     3481 cagcaccagg ctgaatgcgg taaacgccgt cagggagatg gcgcaaccaa tcgccaacgg
     3541 cagattagcc cacagaccca tcacgataga accgagtccg gcaaccagac aggttgcaac
     3601 gaaaactgcc gcaggcggga agcctgcttt acccaacata cctggaacga cgatgaccga
     3661 gtagaccatc gccagaaacg ttgttaaccc ggcaaccact tcctgacgga cagtgcttcc
     3721 acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg gtacgcgctg atggcgtaga
     3781 cataagaaaa catcccctga gagtttaatt ttcgtcaaga tgcgtggcgg acaatcccct
     3841 gccagcgaaa cgttatcttc ctgctccagg tttgcgacaa tttactgcgt cgcaaaaaag
     3901 caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa aagcaaacga ttatccggcg
     3961 atcatagcgg ctttttcaac cgaagtttgc tgcttttttc tcttttattg tcttgacgat
     4021 gaaaaaccag gcaattgatg cgcaaacgtt atcgtcggtg cgcaagattg cagcgttatc
     4081 gagccgtata ccgttcagga ttgcgataaa aaatcgtctg agatgacaaa aatattgcgg
     4141 ataaaaagta tacttttaca ctggcgaaga aatgccggtg acatacaatt aaagcatcaa
     4201 caccaaccgg aacctccacc acgtgctcga atggggtgtg ttgacgtcgg gggaaaccct
     4261 cctgtgtacc agcgggatag agagaaagac aaagaccgga aaacaaacta aagcgccctt
     4321 gtggcgcttt agttttgttc atcttccagc aagcgtgcgc cggtaccttc ttctcctaag
     4381 cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca accaatacat
     4441 ccgtccagtt cgtcacgcag cgctactaag gtatgaatgc gccgatccaa ctcttctcgc
     4501 cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc gggcaacacg
     4561 ccaaacgctt caccaatggt cgccagcgga atgccaatac gctgagcaat tttgataatt
     4621 gcaacatatc gcaacacatc acgtttatat cgccgctgat tgccgctgtt acggatactg
     4681 gtaatcaacc ctttactttc atagaaatgc agcgccgata ccgccacacc gctgcgtttc
     4741 gccacttcgc cgggggttag cagcgcttta atgcggggta atttcttttc cataaatcgc
     4801 tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac gaactgaaca
     4861 ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat cgcatggatt
     4921 gacgagcata ttgaccagcc gcttaacatt gatgtagtcg caaaaaaatc aggctattca
     4981 aagtggtact tgcaacgaat gttccgcacg gtgacgcatc agacgcttgg cgattacatt
     5041 cgccaacgtc gcctgttact ggccgccgtt gagttgcgca ccaccgagcg tccgattttt
     5101 gatatcgcaa tggacctggg ttatgtctcg cagcagacct tctcccgcgt tttccgtcgg
     5161 cagtttgatc gcactcccag cgattatcgc caccgcctgt agttttattg cccgcgcgtt
     5221 aactcccgcg cgggtaattg ctccatccat tgcataaaca cctgcggcgg catcgccttc
     5281 gcaaaaaaac atccctggca atagcgcacg ccgcgtttgc gcagccagtt aacctgctcc
     5341 tcagtttcga cgccttcagc gatcgttttt aaccccaggc tgtgcgccag ctcgatgatg
     5401 tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg tttcaacaaa cgatttatcg
     5461 attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta gccaatacca
     5521 aaatcatcaa ttgccacttc gtaaccagcc tggcggaaag ccagaataat cggcgtcatt
     5581 ttgtcaacat caagaaatgc atgttcagtc acttcaaatt taatttgctg cggacgcacc
     5641 gcgtattgct ctgttttctg attgattcgc gctatcaacc gtgacgtatg aaaatcggag
     5701 gccgacaggt taatagaaac atagcgatcg gcatgtgttg ccaagtacgc gcccagatca
     5761 cggaagacat tatcaataac ataatcagtt atctgttcta tcatcccctc cttttctgcc
     5821 agcggaataa actctgccgg attcattatt tgcccctgct caccaagcca acgtaacaac
     5881 gcttcagcgc cgatacattt ttctgttttg atatcgatta ttggctggta ataaagacaa
     5941 agttgatgtt tttcgagggc gcgttgcaat ttacgtttgg gagataaata gttttgtcga
     6001 atacgtagcc agagtaatag cagaacaaga ctccccaaaa taccggcggg caacgcaaaa
     6061 ataagatgat tataaaaatg ggttataaga cgttgatatg aagtcgcaac aatggctgca
     6121 attgggcgtt ttgtcgaata aactgtcgca tataaatagc catttctttg tacggttaaa
     6181 tccttcaaat gaatcagcgg agaaaacgtt gctgccgagg cctctttgct taacgagaaa
     6241 aaggttttcg tcaccgtatc atacacaccc cattgcaatg tcggatcatc agacatcact
     6301 tcactccaga agagagggtt gataaccgcc acataatttc cccgctgcat ataggtcatt
     6361 ttatagccag agaaaaaagg cgtatcgcgg taataataga tagaaacgtt aggttcacgc
     6421 ttataatcgg ccggtgcaat cgtatagccg tttacaggcg ctatcagcga tgagcataaa
     6481 aaatggttat cacgggcata gatcaattca ttaatataaa gatagccacg aataatattc
     6541 aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc agctgcatcg
     6601 ctcactaaat ctgcctggcg aatgaccagc tcggatttat ccagtgccag ttgagcaaag
     6661 gttcggagct gattattcac ttctgatttt gcccaaagga aggcaatcca tagcgaaaga
     6721 atgatgggaa agagaactaa aaagataatg cccggcaacg ccagtaattg gtgtcgtgca
     6781 cgatgactca ttgctatcaa tttcctgcct gtttataccc ggccattgca aggtgaaaac
     6841 cctgggtacc gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg
     6901 aaattgatac ctttctaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa
     6961 tttcctacag gaatgcagag gcggcgggaa gatatgccct taatgccact gaaaaaccac
     7021 ataatctgta cagcaataaa agtcacggcc taatatggct aacaaacaat caattgtgat
     7081 atagttcaca aaattaatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg
     7141 cgatagccag agtgtaccac gcctcccgtg aacaacgccg cgctgtccag ggcatcggct
     7201 cttttcagag gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc
     7261 aacttcttag tgccgtaatg atgtttctgg ccagcacact caacatccgt tttcgtcggt
     7321 ctgattatgt cgggcttgcg gtgatcagca gcggtctggg cgttgtttcc gcctgttggt
     7381 tcgcaatggg gttgcttggc atcacaatgg cggacatcac cgctatctgg cacaacatcg
     7441 agttggtgat gatagaagag atgaatcaga caccgccaca atggccaatg attttgacct
     7501 gatacgtaga agcctccata acggaggctt cttttttatg gctggcagat gctttaatca
     7561 tccaccttaa aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat
     7621 caaagtccat cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact
     7681 gcgcgccgcc gctgaactga ttgccaccct gcggctgctg tggctgaccc caaccgccct
     7741 gcggctgacc accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca
     7801 gcatctgcat ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg
     7861 attgatcggt ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac
     7921 gcagatattc gctcgccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag
     7981 tctgttcttt catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa
     8041 tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac
     8101 caacgagaat aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc
     8161 gttctgaata agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt
     8221 gacctcggtt ccgggaaaca aacctggcca gacattgtta cacaacactc cgggtaatgc
     8281 attccaatac tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc
     8341 cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga
     8401 tcgaagttcg gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg
     8461 acaagctcat tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca
     8521 ccttatatgc cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt
     8581 ttctgtcact gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct
     8641 caattgagca gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa
     8701 tccacgacta tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg
     8761 acgtcccgct ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg
     8821 aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca
     8881 aaacgctgga gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct
     8941 gcgatctttc cgatccgccg aaactggaac tgcaaaagaa acataccatt gaagtggtgg
     9001 ttgatcgctt caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg
     9061 cgctggagct ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag
     9121 agctgctgtt ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg
     9181 agccgcgact gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg
     9241 gcgtacagca atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg
     9301 gcggtgcgat ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc
     9361 tggcagatca ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc
     9421 ataaagtggt gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc
     9481 gtggcgatac ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc
     9541 gttataaaga gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc
     9601 gcccatgcgc cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg
     9661 agaatacgcc gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca
     9721 acaatctcaa actcgctggt caacgggcga agattgcgga aaaaatcctt aaagagatcg
     9781 gcgatcgttt gaaattcctc gttaacgtcg gcctgaatta cctgacgctt tcccgctcgg
     9841 cagaaacgct ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg
     9901 gcctggttgg cgttatgtac gtgctggacg agccatctat cggcctgcac cagcgcgata
     9961 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
    10021 tggagcacga cgaagacgca attcgcgccg ctgaccatgt gatcgacatt ggcccgggcg
    10081 caggtgttca cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc
    10141 cggagtcgtt gaccgggcag tacatgagcg gcaaacgcaa gattgaagtg ccgaagaaac
    10201 gcgttccggc gaatccggaa aaagtgctga agctgacagg cgcacgcggc aacaacctga
    10261 aggacgtgac gctgacgctg ccggtgggtc tgtttacctg catcaccggg gtttcaggtt
    10321 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
    10381 gggcgaccat cgccgaacca gcaccgtatc gcgatattca ggggctggag catttcgata
    10441 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct
    10501 ataccggcgt gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
    10561 gtggttatac gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc
    10621 agggcgacgg tgtgatcaaa gtagagatgc acttcctgcc ggatatctac gtgccgtgcg
    10681 atcagtgtaa aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa
    10741 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgccgtac
    10801 ctgcactggc gcgtaagctg caaacgttga tggacgttgg cctgacgtac attcgcctgg
    10861 ggcagtccgc aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc
    10921 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
    10981 acttcgccga tattcagcaa ctgctcgacg tactgcataa actgcgcgat cagggcaaca
    11041 ccattgtggt gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
    11101 tgggaccaga aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg
    11161 tcgcggagtg cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa
    11221 ggccgctttc tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc
    11281 cggtagtaaa ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc
    11341 cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggctagg
    11401 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
    11461 aacagctggg cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat
    11521 ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg cctggttttg ccatgcaata
    11581 ttgtagcaac tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg
    11641 cgatccgccg tggatgcgat gttcccccag ccagatgcct tgccaggtgc cggtctgaat
    11701 acgcccttta tgtaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga
    11761 aggcatatcg tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg
    11821 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
    11881 agaggcggag gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc
    11941 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
    12001 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
    12061 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcgtcac
    12121 gctctcccgc tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
    12181 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg
    12241 acatcgcgtg cggcggtaat atcattatca gaatcgccat aaaaaattcg gatattttta
    12301 tcctgcagcc attgcgattt tgtattttgc cctagtttat cgcccgcaaa gatcaccgga
    12361 ttcatgttgg tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
    12421 ttcgtcggac tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca
    12481 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccatt gttcattttt
    12541 tcccagaaca caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg
    12601 cgccagaagc ccggactgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt
    12661 ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aatgggtgcc
    12721 tgttcagcaa gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg
    12781 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc
    12841 ttgcgcatat ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg
    12901 caaaaaccat ctgaccataa cgccagctgc cggggaaagg aaggattatc tgcggttttt
    12961 gtgatgaaaa gcaggatgat tatatttaac ccagtaaata cgttcctgtc tttttttgta
    13021 taaaaaatgt ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc
    13081 ctcggcaaat gagcgaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa
    13141 tgatacagca ttacaaaaac aatcgaagtt tataaagatg atttctgatt gaccagcccc
    13201 ctaccctaca atggtgatta attttattac tcgcgatgga gatgatgata tgaatatatc
    13261 atatgtgaat agcaacaaaa cgacatcttt acctgttgag ctagatgcac taaataataa
    13321 agatatctct tatgccaaag atttttctta tgccaaagat ttctttttat acatagaaac
    13381 acagttaaaa attgcaaaag atttttgtag acctggagaa gaagtatcaa gttctat
//
LOCUS       DAZFRJ010000124        13284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000124 DAZFRJ010000000
VERSION     DAZFRJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.125"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..259
                     /locus_tag="ACHKUN_003235"
     CDS             <1..259
                     /locus_tag="ACHKUN_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6661446.1"
                     /translation="WLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFGQV
                     HYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            286..1980
                     /locus_tag="ACHKUN_003236"
     CDS             286..1980
                     /locus_tag="ACHKUN_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6661447.1"
                     /translation="MFNLPGWLFTVISLVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            2061..2303
                     /locus_tag="ACHKUN_003237"
     CDS             2061..2303
                     /locus_tag="ACHKUN_003237"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO6661448.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            2420..2755
                     /locus_tag="ACHKUN_003238"
     CDS             2420..2755
                     /locus_tag="ACHKUN_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661449.1"
                     /translation="MRTITTHEQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            2912..3979
                     /locus_tag="ACHKUN_003239"
     CDS             2912..3979
                     /locus_tag="ACHKUN_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO6661450.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNTLLTGQQLYGHMDWNRKILTVTTTATPPVDLTAPQGSQKTADTPRNPFRSTTGTA
                     ADQAQVPPAVSASRVQSTTGTSGTSAPRTTTPAPVVTKPVAPPQKVTPPRQWKMEKGT
                     TLKDGLARWTSSEKCSHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEK
                     PLYARTSTAQCLLRVTDRPQE"
     gene            3984..4421
                     /gene="pilM"
                     /locus_tag="ACHKUN_003240"
     CDS             3984..4421
                     /gene="pilM"
                     /locus_tag="ACHKUN_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO6661451.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            4452..6071
                     /locus_tag="ACHKUN_003241"
     CDS             4452..6071
                     /locus_tag="ACHKUN_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO6661452.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            6092..7387
                     /gene="pilO2"
                     /locus_tag="ACHKUN_003242"
     CDS             6092..7387
                     /gene="pilO2"
                     /locus_tag="ACHKUN_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO6661453.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPHYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSVTDENGQIIQPPWKEYDLQIQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            7377..7829
                     /gene="pilP"
                     /locus_tag="ACHKUN_003243"
     CDS             7377..7829
                     /gene="pilP"
                     /locus_tag="ACHKUN_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO6661454.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGGPAEAGRQQESISAVTTNTQASLSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            7914..9422
                     /locus_tag="ACHKUN_003244"
     CDS             7914..9422
                     /locus_tag="ACHKUN_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO6661455.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARIREATTIAGQENGMNPVQNKMLGYIRHAATLNAS
                     DLHITPGRDNTDFTYVEARVHGELEILDIIRKDEGVELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAEKFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSSHIPTFGMLGYHPKQE
                     QTIRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQHGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERRLVETFCNADAVYLRNHDGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            9424..10524
                     /locus_tag="ACHKUN_003245"
     CDS             9424..10524
                     /locus_tag="ACHKUN_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO6661456.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLAIIIRFSLSRYTGPGRRLL
                     DRLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            10598..11134
                     /locus_tag="ACHKUN_003246"
     CDS             10598..11134
                     /locus_tag="ACHKUN_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO6661457.1"
                     /translation="MNFRARLSSYFSLKKDKEEDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNMVD
                     PVTVCSSDKNNIKLETNS"
     gene            11188..11661
                     /locus_tag="ACHKUN_003247"
     CDS             11188..11661
                     /locus_tag="ACHKUN_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6661458.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            11680..12315
                     /locus_tag="ACHKUN_003248"
     CDS             11680..12315
                     /locus_tag="ACHKUN_003248"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO6661459.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDIAGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            12320..>13284
                     /gene="pilV"
                     /locus_tag="ACHKUN_003249"
     CDS             12320..>13284
                     /gene="pilV"
                     /locus_tag="ACHKUN_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO6661460.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHNGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTSGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSAGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCAPDGLVGRTSTGA"
BASE COUNT         3481 a         3604 c         3332 g         2867 t
ORIGIN      
        1 ctggttaccc cgtgatgaaa caacggcgct caccgaggag gcattcatcg ggtgtatggc
       61 caccataacc ggttatcacg cagtggcggg ttccccctgt gaaggtcgct tcacagaccg
      121 ctttgggcag gttcattacc tgttgctgga gccggaggcc gggaaagaat tcagacgagg
      181 agacaaagtg attattgtct gccgtctttc cggttcccgc tggctgggcg aactgaatcc
      241 ctggccggat gttctttaat cccgacatat acagaggttc tcactatgtt taatcttccg
      301 ggatggttgt ttaccgttat ttcgctggtc ctcaccctgt tcgttatcgg gctgatattt
      361 gcccgcctgt accgtcgggc atcggcagag caggcctttg tccgcacagg tctgggaggc
      421 caaaaagtgg taatgagtgg cggtgcaatt gttatgccca tttttcacga aacaattccc
      481 gtcaacatga acaccctgaa gctggaagtc agtcgtgcag cggctgaaag tctcattacc
      541 cgcgaccgca tgcgtgttga tgttgccgtt gccttttttc ttcgggtaaa accatcagcc
      601 gaaggaatat ccactgcagc ccagacgctg ggacaacgca cgctcacacc tgaagatctg
      661 cgtagtctgg tcgaggataa atttgttgat gccctgcggg ccacggcggc cagaatgagc
      721 atgcaggatt tacaggatgc ccgcgagaac tttgttcagg gtgtgcagaa caccgttgcg
      781 gaagacttat ccaaaaacgg tctggagctt gaaagtgtgt cgctgacaag tttcaaccag
      841 actgcacggg aacattttaa tcctgacaat gcctttgatg ctgaaggcct gacccttctg
      901 acccaggaaa cagaacgtcg tcgccgcgaa cgtaacgaag ttgagcagga tgttgaagtg
      961 gccatcaggg aaaaaaaccg ggatgcactc tcccgacgtc tggagattga acagcaggaa
     1021 gccttcatga ctctggagca acaacaacgg gtaaaaaccc gtacggcaga gcagagtgcc
     1081 agtattgctg ctattgaagc tgaacgacgt cgtgaagcag aatccgcgag aattctggct
     1141 gaacgtaaga tagaggaagc cgaaatcgaa cgacagcaga tagtgcgtac ccgccaggtc
     1201 gaagccgaac gggaagtggc catacgggaa attgagcaac agcaggcaac cgaaatcgcc
     1261 agccaggcca gagccattgc cgtggctgca aaatcggaag agcagtcaca ggcagaagcc
     1321 cgggcaagta aagcactggc tgaagccgta caggcccagc aggatgtgga aaccacccgc
     1381 cagactgcgg aggccgaccg taccaaacag gttgccctga ttgctgctgc tcaggaagct
     1441 gaaacgaaag cggttgaact gacggttcgc gcacaggctg aaaaagaagc agccgaactc
     1501 caggcagcag caattattga actggcggag gccacccgtc agaaaggtct ggcagaagca
     1561 gaagcacagc gcgcactgaa cgatgccatt aacgtgcttt cggatgagca gaccagcctc
     1621 cgcttccgtc ttgccctgct ggatgcatta cctgccgtca tagagaagtc cacagaacca
     1681 atgaaatcca ttgacggcat taaaatcatt caggtggatg gcctgaatgg cggtgttacc
     1741 cgtggtaaaa cagcaacaac cgacataagt ggcggaaatc tcgctgaaca ggcggtaacc
     1801 gccgctctgg cttaccgtac acaggctccg ctcatcgact ccctgctgaa ggaggtgggg
     1861 ttatccggtg aaggacttac agccctgaca cgtccactgg tcatgccagc cccggaaaag
     1921 gtcccggatg tcagcgaacc ggtaaccgct ccggataact cagaaccaca acaggtttaa
     1981 tccccgtgcc ggaatttttc cggcatcttt ttcacaaaga atgccctcac gggatattct
     2041 ggtttaaaag aggattgaaa atgtccgcag aaaaattcag agttgtcata tacaccaata
     2101 catgtgaaaa gaaagtcagt tatattcctg caacagaaga tataaacatc gctataatgc
     2161 catttcagac ggcagacaat ggcttaatta ttgtgtgtca gaactcccgt ataatacgac
     2221 gcaaagagcg tatacccgga agcagctgtg aatggaaaga tgtgacatta gcaccaaaat
     2281 ccagaaaccg cgttcagtta tagcctgaca gcctgctttt tatccctcag aaaaggggga
     2341 taaaaattaa atatttcaca accgagatat ttttcaggaa tgcagattat tttatgttat
     2401 gaccaacaga gaaacaggca tgagaacaat cacaacgcat gaacaattat taattaatgg
     2461 gagagtccac gaaagaatcg ccacacatat agttacagga gctcatgggt atgaaacgct
     2521 ttgcacaaat ggctataacg tcagatacaa caaggcgcag caactggttg aaagctgcga
     2581 aaaaatagct gaaggcaaac tacctgttac ctgtcctgtc tgtttcgcca tatggcagga
     2641 tgtacacagg ttcacccacg ttgactttga cacacaatcg gggaaaagtg attttattga
     2701 tacctgccat tcggagatca taaccggaaa tgtttcaata acaggcaaga aataaaataa
     2761 tgctccagtt ctgaaatgca attcactctt ataatccccc cacgaacatg aacgttcatt
     2821 atcaataacc cgatgaaatg atattcatct gaaggccagt tacccgccac ctgtcttata
     2881 ccgtaacccg taatataccg gattttattc tatgcaaaaa aaatcactta cactccttct
     2941 gccgtcatta ctcagcggct gtacggcatt taatgccacg cctgttcctg acaccacacc
     3001 tccggctctg gtgttcgtcg acggacaaat tcgcgaaagc gctgccatta tcgcccatac
     3061 gcaaaaacgt ctgcttccgc caccggtgac ccgccctgtg acgccgcccc gcccgacaac
     3121 aacagcgacg cccacaaccg cagtcagcag ccagagtacc gcactgcctc cccgcccgct
     3181 ccttcaggcc acacagctgc ccgcactggt tatgaccggt aaacccggtc agtcatccgt
     3241 cgtttattta cgtgaggcac gtcagcgctc cgtcagtgac tggatacgcc agttgctacc
     3301 acccggctgg cagttccggc aacagaacgc tgccataccg gcactgaaca aacgcctccc
     3361 cgtctggtcc gttaatgacc agtggcaccg ggcactgaat actctgctga ccgggcagca
     3421 actgtacgga catatggact ggaacaggaa aatactgact gtcacgacca ctgcaacacc
     3481 gcctgttgac ctcacggcac cgcagggttc acagaaaaca gcggacacac cacgtaatcc
     3541 gttcaggagc acaaccggaa cggctgcaga ccaggcacag gtaccacctg ccgtcagcgc
     3601 ttcccgggtt cagagcacaa cagggacatc cggtacatct gcgccccgca caaccacgcc
     3661 agccccggtg gtcacgaaac cggtagcacc accacagaag gtcacaccgc cacggcagtg
     3721 gaaaatggaa aaagggacca cgctgaagga cggactggcc cgctggacgt cgtccgaaaa
     3781 atgctcccac ggcacctgga cgctgcgctg ggaaacgccc gttaactaca gtatcgatgc
     3841 ccctctggtt ttcaccggca gtttccgcca ggcgctggaa cagctcttcg cactctacac
     3901 cccggtggaa aaaccgctgt atgcccggac aagcacagca caatgtctgc tgcgggtgac
     3961 agatcgtcca caggaataac cttatgggct ggttcatcgt gttcatcatg gtcatcacga
     4021 ccacaggcaa tttctggttc accagtcagc tctcccgcag cgaacaccgt catcaggccg
     4081 cagaaaacac acaacaggca gcaacgttca ttcgttatat gaacgccatc aatgattacc
     4141 tgcatcagca tccggaacga cggacagccg gagggcggct gacatccgct cagctcggga
     4201 ttcccgggac ggataccgtc agtcatatca tcagccagca acgtgttttt gtctgggcta
     4261 cagaaacccc gggcctgatg gccgcgctca gggaacagag taatgactct gctctgctcg
     4321 gcagggtgga aaacggccga ctgctggaca cagccggcag agcgctttcc gtcacacttc
     4381 cgtcagtgat ccccgatcac gtcatcctct ggatgaatta gtcacgaatc tgaataaatc
     4441 acggatctcc catgaataaa aaaattcttg ccattatgat tgccgccgct ctgttacagg
     4501 gctgtgccag ttacagacag cttgagagca caagaaagga agccgttgcg gaacaacaac
     4561 gggcacggga aatgatgtct gatctgcgaa cccggacgcc ggttgttaag gaatcctcac
     4621 gacaatggat caatccccgt ccggtcatgg ataaaaacac cggcaaaacc gttcccggtt
     4681 gtcccattgt cattcatacc cgtcagagca tcacccttca gcaggtggtt cagcggatca
     4741 cggaaagctg ccatattccg gtacggatca cacctgatgt actgacatac ctgaatacat
     4801 ccacaaccgg cagcacacaa caaatccagg gcgcattacc cccaccggat gcgagcggca
     4861 tggttcccct tgccgccatt ggcgcatctg acacgccacc atcccgtctg ccgggaaata
     4921 ccgcagcact gtctgaactg aatgcctccg gcctgacgac cctgctgaat gcggtctcct
     4981 cccgtctcgg tatatcctgg cgctatgacg gctcgcatat catcttctat tatctcgaga
     5041 cacagacctt ccccatcacc tatatggaca gtcaggtggc ctataacgcc actgtggtca
     5101 gcggcaccat gtcctcaaac ggctcatcag gaggcagtgc atcagatgcc atttccggtg
     5161 atgccagcaa tacccagacc accaccgtgg atatgaagtc tgcgctgtat caggatgtga
     5221 aaaatgcggt cacatcaatg ctgaccccgg gtatcggacg catgttcatg tccacgggct
     5281 ttatcaccgt cacagataat ccacaggttc tggaaacggt ccgggcattc attgaaaaac
     5341 gcaatgaaga gatgaaacgt caggtggtgc tgaatgtgga aattctgagt atccggaaaa
     5401 cccgcaatga gcaggccggt atcgactgga acgcagtctt cagcgacaga acgctgggac
     5461 tgagtctcgg cagcacattc accagtgcag ccagtgatgc catcacaggt ggtgtttcca
     5521 ttgtggacgg taaactcacc ggttcaaaag ccttcctgaa agcattgtct tcccaggggg
     5581 atgtcagtgt ggtgacccag aactctgccg tcaccaaaaa cctgacgccg gtgccaatgc
     5641 agatcgccaa tcagcaaagt tacatcgaga gtgtgacgac ggataccaca gccaatgtcg
     5701 gctcatccac ctccctgaat gcggccacca tcaccaccgg ttttaacatg acccttctgc
     5761 cgttcattct cccggactca cagacactgc agttgctcta ttccatgagt ctctccgaca
     5821 aaccggttat tgagaattat gagagcggtg gaagtaaagc ccagctgccg aatgtggatc
     5881 tgaaaaccat caatcagacc gtggatctga aatccgggca gacggtcatt atcagtggct
     5941 tccagcagtc aggacgtcgc agcggaaaac agggtgtagg cacgccggga ttcttcggtc
     6001 ttggcggtgg gatcaacagt gaaaacgacg acaccattct cgtggtgctg atcacgccca
     6061 atatcatctg atttgcacaa ggaggacgat cgtgacggat atttcaccga cagggcggca
     6121 ggtgtttccg ctgacggcag ggcgcaatgt gtttctggcc ggtctggaat ggaaaacact
     6181 gccctcacag taccggcatg cccgcgactt tgcccgggca cagaaagccg accttttcct
     6241 ggcctgtcag tatctcagta atgaagatgc cgatacccac accatggtag ccactgtcag
     6301 tcgccgcata ctgcccggaa aaccccgtca gtgcttctcc ctcgccctgc tgatcctgcc
     6361 actacttgaa catggcggct atgccatcac ggaactgacg ttacccggag aaacaccgca
     6421 ctatagcttt gtttccgttg tcgacggtgt tctcgtcagt gacctggtgg gaagcgggga
     6481 agaggttcgg gaagcccggg atacctttct ttccatcaac accgaaccga ctcagggctg
     6541 gatacactat gaaccagcgg cattttcagc aggcgctcag aatcagcacc tgccgctggc
     6601 ctccctgact ggctctggta aacatccggc tgccgcacgc ctgaatccgg tatcccgggg
     6661 accgcagttc atcactgtct ctctgatcat cgcccttctc ggcactgcat ggtacggctg
     6721 gcagtattat gaacgctggc agacagaaaa agccgcgcag gcagcggttg cccgacaggc
     6781 cacagaggaa cgaatcactc ccccctggcc cggactccct gaaacagacg cgttcatccg
     6841 gggatgtgct gatatatgga cttcactgcc actgtctgct gccggatggc gttattcgct
     6901 ggcagaatgt tcgactgacg gtggcagcgg caatcttcgc gcatcgtaca ccaatactgc
     6961 gggcgcgaca gtgactgact tcatccggcg catcagtgat atgacagaca gccgaccgtt
     7021 tattgtgatg ccggaaggaa agaccggcgg cattggcctg ccggtcacct tccggctgtc
     7081 tgaaaacgcc gtcacagttg atgcactacc ggaaaccgct gtcctgcagg aacgactgac
     7141 ctcgctggct cagtcaatgc aactgaaact ggactggcag gaagttaata acagcgtcac
     7201 tgatgagaac ggacagatca ttcagccccc atggaaagag tatgaccttc agatacagac
     7261 cacactccct gcacaccatc tggctgaacg cctgaaagag ccttctgtcc gttttatctc
     7321 tgtcacacgg caactggaga acggtcgttt ccactatcaa tttacaggta aatattatgc
     7381 ccgataaagc caaaaccctt ctgttttcag tcctgctgtt accggccacg gcaatggcca
     7441 caaccggaaa tgcagagcaa cagaacacac agcaaataag gattgaacag atacaggaag
     7501 agacagccct gtatgaagcc atgctggcaa gagagaagat ccgccatgaa ctgcgaacac
     7561 ttgagcaggg ggggcccgca gaggccggac gtcagcagga aagcatttcc gcagtcacaa
     7621 caaatacaca ggcatcacta tccccccgcc tgacagcaat attcggtgtt cagcctcagc
     7681 tgactgcccg gatagaactt ccggggggaa gcatctccga tgtgaaggcg ggccagaaca
     7741 tccccggaac cacttatcac gtggaaaaaa tcacgccgga cagtgtggtg ctggaacagt
     7801 ctggcaccag aaaaaccctg cgaccatgag accggccgcc gtaactggcg gctgtcaaaa
     7861 ataaaaggat gaaagacata ttttccgggc accgctgccc gtaaggaggc tttatgctgg
     7921 atccagcaga cctgccgccg gatgacattc tggattatgt ggccatcgac accgataacg
     7981 ccggacaact gcggatcagg gttgttgagg gaaaaaaaca tctgcgtgcc gtccaggaat
     8041 atctgacccg cctgcgcgca cgctatcagg gacgagtcgg agactttgaa ttcaccacac
     8101 tggatgtgat agcccggatc cgggaagcaa caacaatcgc cggacaggaa aacggcatga
     8161 atcctgtgca gaataaaatg ctcgggtata tccgacatgc cgccaccctt aatgcctccg
     8221 atcttcatat cactccgggc cgggacaaca cagacttcac ctatgtggag gcccgtgtac
     8281 atggggaact ggaaatcctc gacattatcc ggaaagatga gggagtggag cttctggggg
     8341 ccacctactc cgggatgact gacgtcatca agggaacaca atttgatcct ggtgttcccc
     8401 aggacgcccg actggcggaa aagtttctga aacttgcggg gttgtttggc gcacgttact
     8461 cccattatcc ctgcgtgggg ggcctttatg ctgttctgag gctgatcaag gacgacagca
     8521 gccatattcc gaccttcggt atgctgggct atcatccaaa gcaggagcag accatccgcc
     8581 gtattctgca acgaccggaa ggtatcatca ccctgtcagg gccgaccggc tccggaaaat
     8641 ccaccacgct gaggacggcg tctgcggcct atcttgaaca gcacggattc aacaacaccg
     8701 gtggtattct tttgccccgt cgtcgtctct tcaccattga atcaccgccg gaaggacgta
     8761 ttcccggcgc aatccagacc gccgtcatgg acagtgctca gggctgggtg gattccataa
     8821 aatcagccct gcggcttgat cctgacggca ttctgaacgg tgaaatccgc gatcacgatt
     8881 ccgccattac ggcaataaaa gccgccatga caggccatct gatgctgacc accatccatg
     8941 cgaacgaccc gattaacatc ctcgaacgcc ttgagatgga aggtgttcag gccaggatga
     9001 ttgcagaccc gcagttgttc atcggactgc tcagtcagcg tctggttcag ctgatatgtc
     9061 cccactgccg tcatccatgg aatgacgtgg catcccgtcg tactgaggat gaacgacgtc
     9121 tggtggaaac cttctgtaat gctgatgcgg tatatctgag aaaccatgac ggctgcccac
     9181 actgctggcg tggtgtcaca gggcgcacag tgattgcaga agtcatctct cctgatgcca
     9241 gattttttca gttgtaccgg gaaaaaggcc gcatcgaagc caaaacctac tggcacagag
     9301 agctgggcgg gatgacgcgt aatcagcatc tgctgggaaa aatcaacagc ggactggttg
     9361 accctctggc ggcccattac atatctccca ttgatgagga cagctatacg ctgctgcgct
     9421 gatatgaaat ttctggaaaa actcaacagt actgaactgg atctgtctgc cctgaacagc
     9481 tggacagcca gaaaaacctt cggtcttaag cgtgccgagt tctatgagtc cctcggcgga
     9541 atgatacgtg acggaacgcc gtcactgcgc gccctggagt ttatctgtaa tgtcgaaact
     9601 gactttggca cccggaaagg aacatccggc atgtactttc tggccagaga atgtattgcc
     9661 tccctgaaag ccagcggtat gctgtcgggg gccctgccca catgggttcc cccggaagaa
     9721 gcggcactga tcaggaacgg tgaagaacgc ggaaacatcg ccgaagccct gttccaggtc
     9781 gcaaaaaccg tgaaagcacg gcgggaaatg gtgagcagtc tcatcacact ctgtctttat
     9841 ccccttctgc tgctttcact ctgtgtggtg aatatgtaca acgcacatac ccgcatcatt
     9901 cccattgtca gcgcttttgc accggaggag aaatggtcat tccagatgaa agtccttgca
     9961 gccatgtcag agctggtcat cggctacggt ctgtggctca gtggtgccat catcacgctt
    10021 gccatcatca tccgcttctc actcagtcgc tataccggcc cgggacgcag acttcttgac
    10081 agactgccac catggtcgct ctatcagact tttcatggtg tgaattatct gttcagcata
    10141 tcgtcaatgc tgcagataaa tattccgctt tcacaggcgc tgctcagaat tgaaaaaagt
    10201 gcggagcata ataaatggtt aaaggagaga attcaggcaa taagaaaaaa tgtattatcc
    10261 ggtcagaatc tggccaccgc catgcgcaat tctgactatg attttccttc aaaaatatgc
    10321 atcaataaac ttctcttaaa ttctgaaagg gaagacagcg tatctgcaat gaatacctat
    10381 gccggacagt ggctggaaga tgcaaaaaag agcgtcagaa aaagtggatt aattatcacc
    10441 ggtatttcag gaggttttgt attctttttt attcttaaca tgattagcgc gatatacagt
    10501 atcagcgata tgctcaagca ataatacctg ccgtaacact aaaacgccct tcaatatact
    10561 tccattaacg taacaaccat taatacagga gtcatatatg aacttcagag cacgtctgtc
    10621 ttcttatttt tctttaaaaa aagataaaga agaagataaa ggtttaacat tattagaaat
    10681 tgccggcgca atgattatca tcgcaatagt tgtcgcgctc gcgatgagcc agatgtccag
    10741 cacaatggcc tccagtgata accagcgcga actgaaaaac ctgcagacaa ttgcgaccaa
    10801 aatgaagcaa tacaagttcc agggaagata taccggaacg gattatatca aaaccctgac
    10861 ggaaagcggt gcattgcctg ctgatatgat tgcaggaggc aacaaagcca aaaacgcatg
    10921 gggtggtgat gtcacgattg ctgccacagc agacaaatat ggctacatta tcaccagtaa
    10981 taatgttcct aaaacaaact gtgttgagct gattaacagc ctgcgcagtt cgtccatatt
    11041 taccaaaatt atgaacacag ccccgaacat ggtggatccc gttaccgtct gcagtagcga
    11101 taaaaacaat atcaagctgg aaaccaacag ctaatgccct gccacacagc actccctccg
    11161 gggtgctgtt tcccggagca cataatcatg aaatggtttt atctctcatc tcttttctta
    11221 ttctgtctcc cggcgactgc cgccgtaaac agtaacacac tggatagctg ctttattcag
    11281 gcaggaaaac gttactccat cgatcccgga ctactgaaag taattgcccg taaagaaagc
    11341 agcctgaatc cccgggccat caatcataac agaaatgcca gcggaaaaat tatcagcaca
    11401 gattatggcc tgatgcagat taattccaca catattcccg gactgaaaag aatgggaatt
    11461 atccgaagtg aaaatgaact gctcagtaat gtttgcctga atatacagac aggtgcatgg
    11521 atccttgccc ggcattttca gcagtgtggt gtgaactggg agtgtctcgg tagctacaac
    11581 gccggattta ataaaaataa cactcaccgc agaatgaaat atgcccggct gatttatgcc
    11641 gcatatatgc agggacgtta ataacggaga atacgagcca tgttcctcat catcttactc
    11701 gtgatctccg ctacgctgac catacagtta cttgccatca gactggccgg acattatctc
    11761 atgcaggtgc ataccctccg gcgtccacgt cacctctccc tgctctgtgc tgttctggca
    11821 ctcaccgcca ccattacgca catcatacgg gggacagaca ttgccggact ttgctttctg
    11881 ttgctgatgg ccggctgcat gacactgatg acactgactg atgcagcctc ctgccgtctt
    11941 ccccgtccct tcaccctcac attcctgatg ctgggtgtgg cgtttcgttt atggcagggc
    12001 gatgctgcta ttgcgcttgc ctgtgctctg ttctggtttc tcctgttatc tctcctgagg
    12061 cgctacacct cataccgcaa gggagtggag acgctgggac tgggggacgt gtttctgatt
    12121 gcaggtgtgg ccatgtggtc atctgccaac agcactccgt ggatcatcgg tattgccgcc
    12181 gccagcacaa tgatctgtat gtcgctgttc ccgcgcagta atactggggc cgctattccg
    12241 ttcggtccgt ttatctgtgc atcactctac attttcactt tttttcctgg tgccattttt
    12301 cacaccggag ggtaacacca tgcaaaaaga cgacgataaa ggttttgttc tgtttgaaat
    12361 tctggccgga ctgattgtga tcggtatcgc cacaccaatg atttattcag aaattgaaaa
    12421 ctggctgaat gagcagcttt accagagtgc tgcatatcat gcagatgcct acaataccgc
    12481 agccagaaac tatattgccg acaacaatgc ccgtctgcat aacggcagcc ttccggcaaa
    12541 tttcacggct gatgacctga ttcgtcaggg atatctgaaa caggggttta accattctcc
    12601 gtttggtcag agttatatca ccggtatccg ccgtaaccag acctccgggc gtctggaagc
    12661 cctgacctgc tcaactggag ggcagacaat caaagaagaa gggctgcgca gcgtggcggg
    12721 tcagttgccg gggctgggtg gatttatcag caaaaacgga acggcaacag gcgcattcgg
    12781 agcctggaca gataaaccgg gagactacgg acttacctgt tcggcaggtc acatcgccgt
    12841 cgtcatgagc ggtgatgacc tgcaggaaag tgaccgtctc taccgttttc aggtagcagg
    12901 tcgtcctgaa ctgaatcaga tgcataccgc catcaatatg ggcgggaata acataaacaa
    12961 caccggcaat atcaatggtc agagtgccac actgaaaggt gatattacga gtgaagacgg
    13021 ctggctaatc accagaaaca ataagggatg gatgaatatc acacatggtg gtggattcac
    13081 catgacagac agccagtgga tacgcgcggt taatggcaaa ggcataacaa ccaccgggga
    13141 aatcaaaggc ggaaaagtca gtggtggtac agtccggtct gacggtcgtc tttcaaccgg
    13201 ggagtattta cagcttgata aaacagctac ggcaggcaca aagtgtgcac cggacggact
    13261 tgtaggccgc accagtacag gggc
//
LOCUS       DAZFRJ010000125        13275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000125 DAZFRJ010000000
VERSION     DAZFRJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.126"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..189)
                     /locus_tag="ACHKUN_003250"
     CDS             complement(37..189)
                     /locus_tag="ACHKUN_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6661461.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHKUN_003251"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHKUN_003251"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6661462.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHKUN_003252"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHKUN_003252"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO6661463.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHKUN_003253"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHKUN_003253"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO6661464.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACHKUN_003254"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACHKUN_003254"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO6661465.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHKUN_003255"
     CDS             5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHKUN_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6661466.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHKUN_003256"
     CDS             6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHKUN_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6661467.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHKUN_003257"
     CDS             7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHKUN_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO6661468.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHKUN_003258"
     CDS             complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHKUN_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6661469.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHKUN_003259"
     CDS             complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHKUN_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO6661470.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHKUN_003260"
     CDS             complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHKUN_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6661471.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHKUN_003261"
     CDS             complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHKUN_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO6661472.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHKUN_003262"
                     /pseudo
     CDS             complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHKUN_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         3094 a         3618 c         3354 g         3209 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcacctac
      121 catcagcgtg aatcccagta ctgtacaaca cagtacgatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgtaagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      541 atgggtatcg cccaccgata aatatccttc gtcccataat gggtgatatt tcaggccatg
      601 tttttgcaga tactgatata tcgttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg
      721 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
      841 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
      961 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc cggcgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctctgcttcc gccatcgcca gcgggcaagt cgggaacgag acgcaggcca
     1681 tcgagttttc acgctgcggc gtgacggcat tcattaaccc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg
     2281 catgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
     2341 ggtaggtctg accaaggatc ggttcttcat cagtagtggc gactttttcg tggtcgagcc
     2401 agatctccgc atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact
     2461 cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
     2701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttgtcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt
     3241 cagcacgcca tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa
     3301 gtgcggatta ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
     3421 cgggttagcg ggcaggcgaa agttatcgtt atgttcgata aatacgcgga cttcgccctc
     3481 ttcttccacg cgatccgcga ggaagctgga ggcaccaccg gcacgggctc ggccttccac
     3541 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
     3601 ggcgatggaa tacaggcgcg gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc
     3661 cagttgcgcc ggagagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgcccg gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
     4081 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acatcggcat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
     4261 cttgctgtcg aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
     4321 aaacacggca aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcttccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
     4501 cagttttgct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
     4561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gctggcgctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtgggagtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac taaaagcggc gtttaaacca
     5281 acctacgagc gcctcgatca ccattatctc aatgatattc caggtctgga aaacccaacc
     5341 agcgaggttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgtaccgca ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctcccgca atttcatctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa gaagaaggtg
     5881 tcgaatgcat ccccggagcg acggtggatg cactgtatga agaaaacggc agggtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgtg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgagcgt cacgggttag tgactcgtcc tgctggtgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgctggaac catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcaggat ctgtgatgac ttacccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
     6241 cccttacgca aagtcgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
     6481 ctggcgcgca ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa
     6541 atctgtttgc gctttatcat cacaacgtag agcgcagcct gctatgggat gttctacaac
     6601 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
     6781 tcaggagtct gcgatgtctg tagcccataa tctctggcgc gttgctgatg cgccgcacat
     6841 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgcgtt gattatcacg gctgcctgga
     6961 gtgtggcacc tgctgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggcaatcga ctctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtga ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct
     7501 gggtgacaga gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct
     7621 ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
     7741 caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg
     8101 atttcctggt tatctgcctc accgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcac gggtaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgcg
     8221 ccggggacaa tgttcagttg ctgcatacca gagggcaacg tggcgttttt cactgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca
     8761 gttgcagaca ctgcatagct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caaaccatgt tcaatacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
     9481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
     9541 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct
    10381 cccagaccaa tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatggt cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg
    11161 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac acattttccg
    11461 tctgcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgtgcca
    11701 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc attttccagc acttcctggg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggaacc gtcgagcacc
    12001 accgagtttt ctacaacagt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagacttg ttctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc gagataaaga catagtaatt
    12301 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    12361 gattagcgca ctaaataacc accatcgacc actaataaat gcccattgac ataatttgaa
    12421 gccggacttg cgaggaatac agctgcgccc attaaatcct gagtatcgcc ccaacggttt
    12481 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta
    12541 atatctgttg cataatagcc aggggcaata ccatttacct gaatattata ttgacctagt
    12601 tcatcacaat aagctttggt gaatccggca agagcatgtt tagtggcaga atatgcaggt
    12661 gaccattgtc cacctaagta agagaacaat gaacagatat taataatttt gccgcttttc
    12721 tgcgggatca taatttttgc agcttcatag cttaactcga atgcggcggt caggttcaca
    12781 tcaatcatcg gatcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata
    12841 ccggcattgt ttaccagaat atcaactgta ccgaaacgct cacagcaagc agcgataatc
    12901 ttctgcggcg cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctgt
    12961 ttttcaatca tttcctttgt ttcgccgtta tctttgacga aactaggaat aaagatattt
    13021 gcgccagctt tggccaacgc catggcaaat gcctagccta aaccgctatt cccaccggta
    13081 acaattgcgg ttttaccttt cagtgagaaa aaatccattg agaacgcatt gagagattcg
    13141 attgacatag ttagctccaa tttccgtaag gcaaaaaaaa gaaaggtaac ttcccccgcg
    13201 agagggaagt tacctttctc attatctctg gtaactggag ataattttta gcataacggc
    13261 ataactgcta atgtg
//
LOCUS       DAZFRJ010000126        13088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000126 DAZFRJ010000000
VERSION     DAZFRJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.127"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..289)
                     /gene="cspH"
                     /locus_tag="ACHKUN_003263"
     CDS             complement(77..289)
                     /gene="cspH"
                     /locus_tag="ACHKUN_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO6661473.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            575..787
                     /gene="cspG"
                     /locus_tag="ACHKUN_003264"
     CDS             575..787
                     /gene="cspG"
                     /locus_tag="ACHKUN_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO6661474.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            798..986
                     /gene="ymcF"
                     /locus_tag="ACHKUN_003265"
     CDS             798..986
                     /gene="ymcF"
                     /locus_tag="ACHKUN_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6661475.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            961..1191
                     /gene="ymcE"
                     /locus_tag="ACHKUN_003266"
     CDS             961..1191
                     /gene="ymcE"
                     /locus_tag="ACHKUN_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO6661476.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            1181..1354
                     /gene="gnsA"
                     /locus_tag="ACHKUN_003267"
     CDS             1181..1354
                     /gene="gnsA"
                     /locus_tag="ACHKUN_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO6661477.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1402..2475)
                     /gene="yccM"
                     /locus_tag="ACHKUN_003268"
     CDS             complement(1402..2475)
                     /gene="yccM"
                     /locus_tag="ACHKUN_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6661478.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIAISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2547..5291)
                     /gene="torS"
                     /locus_tag="ACHKUN_003269"
     CDS             complement(2547..5291)
                     /gene="torS"
                     /locus_tag="ACHKUN_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO6661479.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            5374..6402
                     /gene="torT"
                     /locus_tag="ACHKUN_003270"
     CDS             5374..6402
                     /gene="torT"
                     /locus_tag="ACHKUN_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO6661480.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6375..7067)
                     /gene="torR"
                     /locus_tag="ACHKUN_003271"
     CDS             complement(6375..7067)
                     /gene="torR"
                     /locus_tag="ACHKUN_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO6661481.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7197..8369
                     /gene="torC"
                     /locus_tag="ACHKUN_003272"
     CDS             7197..8369
                     /gene="torC"
                     /locus_tag="ACHKUN_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO6661482.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8369..10915
                     /gene="torA"
                     /locus_tag="ACHKUN_003273"
     CDS             8369..10915
                     /gene="torA"
                     /locus_tag="ACHKUN_003273"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO6661483.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10912..11511
                     /gene="torD"
                     /locus_tag="ACHKUN_003274"
     CDS             10912..11511
                     /gene="torD"
                     /locus_tag="ACHKUN_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO6661484.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVGRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11604..11909)
                     /gene="cbpM"
                     /locus_tag="ACHKUN_003275"
     CDS             complement(11604..11909)
                     /gene="cbpM"
                     /locus_tag="ACHKUN_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO6661485.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(11909..12829)
                     /gene="cbpA"
                     /locus_tag="ACHKUN_003276"
     CDS             complement(11909..12829)
                     /gene="cbpA"
                     /locus_tag="ACHKUN_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO6661486.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3181 a         3317 c         3429 g         3161 t
ORIGIN      
        1 ctggtgttat aatgtatgat gttgactatt gcactattcc aataaaacca gtcagcttta
       61 aacaagcagc gtcatattaa gagagataaa catttgccgc tgttggtcct cgcaggccat
      121 ttacgcggca aaattccaca cgtaatcctg gtataagcac ttctgcgtcg cggggagtga
      181 atgcggaaat atggacctga acttctttac gaccgtcgga ggggataatg aatcctttgc
      241 cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt acgggacaaa caaattcctt
      301 actgaaaata ctgcgctgca ctatacgggg ttaataaaat aaagccagcg atatttaaga
      361 ccgccggacg gctaaaataa aatttgctta atctcaatta tcatgcgtta atagctgcgt
      421 cggtttgaaa gacagacagc atacaaagta gtttactaaa gcagttctca ttatcaggca
      481 ttatcccctt cttttgagtc tctctcctga acactaagta gtttctgtat taaagccctg
      541 tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct aataaaatga ctggtttagt
      601 aaaatggttt aacgcagata aaggttttgg ctttatcact cctgatgatg gcagcaaaga
      661 cgttttcgtc catttcactg ccatccagag caatgaattc cgcacgctga acgaaaatca
      721 gaaagttgaa ttttctattg agcaggggca acgtggcccc gcggcagcga acgttgttac
      781 gctctaaggt tgccgttatt actcaacatc tccatttccg ctgtccatgt tgtcatggtt
      841 cacagtaccg tacatcggca ttcgatgtga cggagcgaaa ccctttgggc gctaagtgta
      901 ttttttgtaa atcgacgatg atcacctttg ataacgtcgc gctgcaaata cgcacggacc
      961 atgcgccgct ggatttcaca aaataatatc aggctccctc gtggagcctt ttttatatct
     1021 gccttatttt tcttcaacgc tgtatgtata gtaagcgata acctgttgat tattgaatct
     1081 ttcggggaga tggcttataa catttcttac ctgaccaggg taccgggaac caacacctta
     1141 ctggcgtgtt gctgtctttc aagaccagaa gaggttaaca gtgaatattg aagagttaaa
     1201 aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa aaaatcgccg agctgaacaa
     1261 gaatacaggg aaagaagtct ctgaaattcg cttcaccgca cgagaaaaaa tgaccgggct
     1321 tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt gaacatcacc cgtgcgaggt
     1381 gatgttccgc ttgttgctaa tttagtgacc aatcattggc gcttgtggaa ttaagcgtcg
     1441 gtacaattca tccggtaccg ggctttgcca tactcccgca tacattgcgt aaccaatcac
     1501 cgcaaacata atccccagaa ccagtagcgt cattaaccag ccagacaacg caaaggcttt
     1561 tttatttgcc gcaggttttt gcagtgaaaa ggtcaatgtt gaggctaccg gacatgactc
     1621 tacgcaagtc atacagccgg tacattccac tgttcgtacc tgaattaatt tatcgaccgg
     1681 gatccgtgat gggcaatttt ttgcgcattt gccacagtcg atacaacttt cggcattgcg
     1741 acgaatctta aacggcgaca atagcgaaac cacgcccatc agcgcgccat atgggcaaag
     1801 ataacgacac caggcatggc gaataaacag gctggcaatc agcaaaacgg tcacgctgat
     1861 taatgtcgcg gtccccatat gacgaaagaa atcgagcatt ttaacgtcca tcaccacgct
     1921 gtagggcgac aacataaaat agtgaatcgc ctgagcgggc atcaataatg cgatatagag
     1981 aaaaaaactc aacagcaaat acttcacgcc gcgcagagga atatccagcc agcggggaag
     2041 gacacattgc cgaccaaaca gtttgttacc gagatcgccg attaattcag aaagcgtacc
     2101 aaccgggcat aaccatgagc aaaaggcctt tttgagtaat agactgatgg cgataaaagc
     2161 gaccaataac agcatcgcgg cggcgtggac ggacggtaac tgacctgttg taaggctata
     2221 tttcagattc atcagaccgg caatcggtag ccagccttcg atacctcccg gtctggcgac
     2281 aaatgtcgtg ctacttgccg tttcgtaata gcgcacccaa taccagaacg tgatggcaat
     2341 ataaatattc attgccaaca gtaataattg cgtcgcttta cgccaggtcg tggcattgcg
     2401 ccagtcattc cacggtaatt tgccgcccgt cgtgcctggc cgccgctgcc agcgggttct
     2461 ttttttctct gccatgattt tgccagtccg ttaagttgca taccaaatgc cactattcta
     2521 gttgttctta actggctgat attgattcaa atcgcgttca ggtctttctt attcaaccat
     2581 gcttccagat cggcaacact gcgtgtaatt tcttcgtgtg gaaggggggc agataatggc
     2641 tgctgctcca gttgtgcgca tagctggctg gcgatacgca ttcccaaact cgagcaactg
     2701 ctttttagct gatgcgcggc acgctttatt ttctcgctat cctgactggc gcgggcaatg
     2761 tcgatttcat cgagaagcgg cagggcatgt tgtgtaaata ataccagcca ttcgtggatc
     2821 ttctccgtcc ccattaactg agcatcttca ttgagttgcg atacatccag cgattgatcg
     2881 ttattgactt gcagttggag atagtgcgcc agtaactgac cgagcacttc acgcggcacc
     2941 ggtttaggga taatcccgcg gaataatgaa ctggtacgct ggcgcagcgt ttcgtcaatg
     3001 acatgggcgc taaagccaat caaaaccagc gacggatatt gctgtgccag ttgtcgggca
     3061 agcgtaatgc cgtcgacatc cggcagatca aaatccacca gtgcggcagc aaacggttcg
     3121 ctattttgca gtgtctctaa agcctgcgcg gcattgccaa cagcaacaac ctgcgcacca
     3181 ctggtgttca gcatctcaac ggtaattcgc tgggttagcg ggttatcttc aattaacagc
     3241 aaacgtaaac cgtcaagacg caccgcctga ttgactgttt ttggcacggg tgccgtggca
     3301 acacgtaacg gcaagcgtaa acaaaaacag cttccaacct ccggcgtgct ggtggcgctc
     3361 agttcgccgc ccatcgcctg ggccagacgg ctactgatag tcagccccag cccggtgccg
     3421 ccgcgtttgc cgcttacctg cacaaatggc tggaagattt ctgccagttt cgcgggatca
     3481 ataccgcagc cgctgtcttc cacttcgacc agccattgct cgccatcagt gcgactacgc
     3541 aggataatgt acccttcgtc agtaaaacgc agggcgttgc tcaacaggtt ggttataacc
     3601 tgacgaatac gtcgtggatc gcccattaac gcgcacggca tatcatcggc aattgccgtt
     3661 gccaggcgaa tcgggcgacc tttcacccgt ccgctcatta attgcagggt actttccagc
     3721 agcgggcgcg gttcaaaggg ctcatcgctg accgaaacat tcttgccacc tgcttcgata
     3781 gcggaataat cgagaatatc gttgaggatg gtcagcaacg attcaccaga atcagtaatt
     3841 gcccgcaaat catcacgctg ggcgttaagt gcggggttat ctgccagcag ttgagcagtg
     3901 ccgagaatac cgtacagcgg tgtgcggatc tcatggctca tcgccgccag aaacgccgat
     3961 tttgcctggc tggctttttc tgcttccgcc cgtgcctgtc ggtgttctat caccagttcc
     4021 tgcaattcag ctgtacgcgc tttgacctgc gccgccagct gttcgcggtg gcgattcagt
     4081 gcatgaacat tgctgcgaaa cgcatccatc agccgcccga tggtatccag ctcccgtacg
     4141 ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca gcagccgttg cagcgcctgc
     4201 gtttgttcgg caagtggacg cgtgactgag cgataaacca cgcgccagag gatcagaatc
     4261 agtgcgcaaa gagaaactat tcccagcaat aacaggctgt attgcccgcg tgcactcgct
     4321 ttttccagat gcgccagtcc gtgctgatta cgcagctcaa tggtatcgac cagctgactg
     4381 acttcgctac taaactgcgc gaactgggcg atgttatttt gggcgagagt ttgcaggtga
     4441 gtgctgattt cactgtcctg ctgatacagc gccagtagat cgctatattg gctaacggta
     4501 gttaacgttg ttgcgacctg cgcacgaact cccggatctt caatgcgtat ttgccgacgt
     4561 tgcagaattt tcaccgcatt attgagctgc ttttccagcg ttggtgcatt tttctggatc
     4621 tgctccagcc ccagattcat caccatttgc tgcacccgca gagcgctaag gcgcagttca
     4681 ttcatctggt taacatactc aagatcgata tcaatcagcc gatcgagtgc actttcagca
     4741 gcctgacgct gatcttgttc gatcaaatcg taaatcccgg cctgggtcgc tccagcggaa
     4801 gttgtcgcat tattcgcctg accttgcgcc aggcgtgcga tctcatcggc ggcagcgact
     4861 atctgctgac tgagttgccg ttgttgctgg cgtagttgca gacgccgccc caccagttcc
     4921 ccttgctgac gtaatgaacg ggagatctcc tgctcctgtt gttcaatagc ggtggtatca
     4981 aaaccttgtt cccgtaacgc ttgcagcaac gcattaatct tcaggctttg tgcggtgagc
     5041 attcgcccct gcgcctgcca catcttttcg ttatcggcac tggtcaggtt ctgcgcggcg
     5101 aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca tattcatggt aggaatcaat
     5161 gcctgagtgt tgtctttttc cacctggctg ataaagcgca ggttgtacca tcccaccagg
     5221 gtactggtca gggttaacag cgccatcagg gcaaagccca tccagagtct tcgggtcagg
     5281 gttaaattca cggtcggtgc acttaaggtg aaaaaggttg agtcgcaaag cggaatgcat
     5341 ctagcataaa gccttattat tgatgaggct atcatgcgcg tactgctatt tttacttctt
     5401 tcccttttca tgttatcggc attttcggct gataacctgt tgcgctggca tgatgcgcag
     5461 cattacacgg tgcaagcctc tacgccgctt aaagccaaac gcgcatggaa actgtgcgcg
     5521 ctttatccca gcctgaaaga ttcatactgg ttatcgttga actatggtat gcaggaggca
     5581 gctcgccgct atggtgtgga tttaaaagtg ctggaggcag gcggctacag ccagttggct
     5641 acccagcaag cacaaatcga ccagtgtaaa cagtggggcg cagaggccat tttgctcggt
     5701 agtagcacta cgtcatttcc cgacctgcaa aagcaggtag caaatctgcc ggtgatcgaa
     5761 ctggtaaatg ctcttgatga tcccctggtg aaaagccgcg ttggtgtacc ctggtttcag
     5821 atgggctatc aacctgggcg atatttggtg caatgggcgc acggtaaacc actgaacgtg
     5881 ctgttgatgc ccggacccga taatgccggg ggcagtaaag agatggtcga gggttttcgc
     5941 gcagccattg ccggaagccc ggtgcgtatt gttgatattg cgcttggtga taacgatatt
     6001 gaaatccagc gtaacctgtt gcaggagatg ctggaacgcc acccagaaat cgacgtcgtt
     6061 gccggaacgg ccattgcggc agaggcggca atgggtgaag ggcgtaacct gaaaacgccg
     6121 cttaccgtgg tgtcgtttta tctttcacat caggtgtatc gcgggctgaa gcggggaaga
     6181 gtgattatgg ctgccagcga tcaaatggtc tggcaggggg aactggcggt tgagcaggcc
     6241 atcaggcaat tacaggggca atcggtgtct gataatgtca gcccaccgat tttagttctg
     6301 acgccgaaaa atgccgaccg tgaacatatt cgccgctcgc tgtcaccagg gggatttcgt
     6361 ccggtctatt tttatcagca cacatcagcg gctaagaaat aaccttcacc atgttgcgtc
     6421 accagtaaat ccgcgctgag tttatgacgt aaacgacgaa ttaacacatc gacggtgcgc
     6481 aggtcagggt tttccacccg acgcgcagaa agcatacgta gcagacgttc acggctgaga
     6541 atttcgcccg gattcgtcac aaatgccacc aacatttcat actctgcgcg ggtcagttta
     6601 atcggctcgc catcccgctc cagcgtatgg cgcgacacat tcaggcaata accggcaaag
     6661 cgatagcagt tgtcctgagt gtgcggttga gcttgtcgcg cgaggtcgat tcgccagagc
     6721 agatttttca cccgtactac cagttcgcgc agttccagcg gtttggtgac gtaatcgtct
     6781 gcgcccattt ccagcccaac aatacggtca atccgatcgc tgcgtccggt aaccagaata
     6841 atccccaccg ttgagcgttc tcgcagggcg cgggttaaca tcaggccatt ttcatcgggt
     6901 aagttgatat ccagcagaat taaatctacc ggctgattct gcataatttc ccgcagcccg
     6961 gcaccgctcg ctgtaacgga aacggtatac ccctcctgag tgaagtaaga ttgtaatcgc
     7021 gcctgggtaa ccggctcatc ttcaacaata acaatgtgat gtggcatcag agggttttac
     7081 tcattctgtt catatctgtt catattctgc cgtaagccgt tcatcctgac cagtgccgct
     7141 gttcatattt gctcattaag atcgcttcac taaaccataa ttttacaggg gctattatgc
     7201 ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg
     7261 caattgggat tgtgattggc attgcgctga ttgtattgcc acacgttggg atcaaagtca
     7321 ccagcacaac cgaattttgt gtcagttgcc acagtatgca accggtgtat gaagaatata
     7381 aacagtcggt gcatttccag aacgcctccg gcgtgcgagc tgaatgccat gactgtcata
     7441 tcccgccgga tattccaggc atggtgaagc gcaaactgga agcgagcaat gatatctacc
     7501 agacctttat tgctcactcc attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac
     7561 ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgc cgctcctgcc
     7621 ataactacga tgcgatggat catgcgaagc agcatcctga agcggcacgt cagatgaagg
     7681 tggcagcgaa agataatcaa tcctgcatcg actgtcataa aggtattgcc caccagttac
     7741 cggatatgag tagcggcttc cgtaagcagt tcgatgagct gcgcgccagt gctaatgaca
     7801 gtggtgacac gctgtactct attgatatta agccaattta tgcggcgaaa ggcgataaag
     7861 aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact
     7921 ggctacaaat tgaaattacc ggctggacgg aaagcgccgg acgtcagcgt gtactcaccc
     7981 aattcccagg taaacgcatc tttgttgcct cgattcgtgg tgatgtgcag cagcaggtaa
     8041 aaacgctgga gaaaaccacc gttgccgaca ccaataccga gtggagcaag ttgcaggcca
     8101 ctgcgtggat gaagaaaggc gacatggtga acgatatcaa accgatctgg gcttatgcgg
     8161 attcgttgta caacggcacc tgtaaccagt gccacggtgc accggaaatc gcccactttg
     8221 acgctaacgg ttggatcggc acgctcaacg gcatgattgg ctttaccagt ctcgataaac
     8281 gtgaagaacg caccttgttg aaatatctgc aaatgaatgc gtctgacacc gcaggtaagg
     8341 ctcacggcga taagaaggaa gaaaaataat gaacaataac gatctctttc aggcatcacg
     8401 tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt
     8461 gttaacgccg cgccgtgcga ctgcggcgca agcggcgact gacgctgtca tctcgaaaga
     8521 gggcattctt accgggtcgc actggggggc tatccgcgcg acggtgaagg atggtcgctt
     8581 tgtggcggca aaaccgttcg aactggataa atatccgtcg aaaatgattg ccggattgcc
     8641 ggatcacgta cacaacgcgg cgcgtattcg ttatccgatg gtacgcgtgg actggctgcg
     8701 taagcgccat ctcagcgata cctcccagcg cggtgataac cgttttgtgc gcgtgagctg
     8761 ggatgaagcc ctcgacatgt tctatgaaga actggaacgc gtgcagaaaa ctcacgggcc
     8821 gagtgccttg ctgaccgcca gtggttggca atcgacgggg atgttccata acgcttcggg
     8881 gatgctggcg aaagctattg ccttgcatgg taatagcgtt ggtacgggcg gagattactc
     8941 taccggtgct gcgcaggtga tcctgccgcg cgtggttggc tcaatggaag tgtatgaaca
     9001 gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc attgtgctgt ggggctctga
     9061 tttgctgaaa aaccagcaag cgaactggtg gtgcccggat cacgatgttt atgaatatta
     9121 cgcgcagcta aaagcgaaag tcgccgccgg tgaaattgag gtcatcagca tcgatccggt
     9181 tgtcacatcc acccatgagt atctggggcg cgagcatgtg aagcacattg cggttaaccc
     9241 gcaaactgat gtgccgctgc aactggcgct ggcgtatacg ctgtacagtg aaaacctgta
     9301 cgacaaaaac ttcctcgcta actactgtgt gggttttgag cagttcctgc cgtacctgct
     9361 gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct gaaaaactga ccggcattga
     9421 tgccgaaacc attcgtgggc tggcgcggca gatggcggcg aacagaacgc aaattattgc
     9481 tggctggtgc gtgcagcgta tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct
     9541 ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggc tttggttttg gctggcacta
     9601 taacggcgca ggcacgccgg ggcgtaaagg cgttattctg agtggtttct ccggctctac
     9661 gtcgattccg cctgttcacg acaacagtga ttacaaaggc tacagcagca ccattccgat
     9721 tgcccgtttt atcgatgcga tcctcgaacc ggggaaagtg atcaactgga acggtaaatc
     9781 ggtaaaactg ccgccgctga aaatgtgtat ttttgccgga actaacccat tccatcgcca
     9841 tcagcagatc aaccgcatta ttgaaggctg gcgcaagctg gaaacggtta tcgccataga
     9901 taaccagtgg acctcaacct gccgctttgc cgatatcgtg ctgcctgcga ccacgcagtt
     9961 tgagcgtaac gatctcgacc agtacggcaa ccactccaac cgtggcatta tcgccatgaa
    10021 acaggtggtg ccgccgcagt tcgaggcgcg caacgacttt gatattttcc gcgagctgtg
    10081 ccgtcgcttt aatcgcgaag aagcctttac cgaagggctg gacgaaatgg gctggctgaa
    10141 acgcatctgg caggaaggtg tacaacaagg caaaggacgc ggcgttcatc tgccagcgtt
    10201 tgatgacttc tggaataaca aagagtacgt cgagtttgac catccgcaga tgtttgttcg
    10261 ccaccaggca ttccgcgaag atccggatct cgaaccgctg ggcacgccga gtggcctgat
    10321 tgagatctac tcgaaaacca tcgccgatat gaactacgac gattgtcagg ggcatccgat
    10381 gtggtttgag aaaatcgaac gctcccacgg tgggcccggc tcgcagaagt atccgttgca
    10441 tctgcaatct gtgcatccgg atttccgact tcactcgcag ttatgtgagt cggaaactct
    10501 gcgtcagcaa tatacggtag cgggtaaaga gccagtgttc attaacccgc aggatgccag
    10561 cgcgcgcggt attcgtaacg gtgatgtggt acgcgtcttt aacgctcgcg gtcaggtgtt
    10621 ggcaggggca gtagtttctg accgctatgc acccggcgtg gcacgaattc acgaaggggc
    10681 atggtacgat ccagataaag gcggcgagcc tggtgcgctg tgcaaatacg gtaaccccaa
    10741 cgtgctgacc atcgacatcg gtacttcgca gctggcgcag gcgaccagtg cgcacactac
    10801 gctggtggaa attgagaagt acaatggagc agtggagcag gtgacggcgt ttaacggccc
    10861 tgtggagatg gtggcgcagt gcgaatatgt tcccgcgtcg caggtgaaat catgaccacg
    10921 ctgacagcac aacagattgc ctgtgtttac gcctggctgg cgcagttgtt ctcccgtgaa
    10981 ctggacgatg aacaactgac gcaaatcgcc agtgcgcaga tggctgaatg gttttcgttg
    11041 ctgaaaagcg aaccgccgct ggctgcggcg gtgaatgagc tggaaaactg tattgccacg
    11101 ctgacagtac gtgacgatgc ccgtctggaa ctggccgctg acttttgcgg cctgtttctg
    11161 atgaccgaca aacaagcggc gctgccgtat gcatcggcct acaaacagga cgagcaagag
    11221 attaaacgct tgttagttga ggcagggatg gaaaccagcg gcaatttcaa cgaaccggca
    11281 gatcatctgg cgatctatct cgaattgctc agccatctgc atttttcgct gggagagggg
    11341 accgttcctg cgcgaagaat cgacagtttg cggcaaaaaa cactgacggc gctgtggcaa
    11401 tggttaccag agtttgttgg gcgttgtcgt cagtatgaca gctttggttt ttacgcggca
    11461 ctaagccagt tattgctggt gttagtggag agcgaccacc aaaacagata acgtcgtttg
    11521 tgcgcctgaa aagacgcgtt tagcgtcgca tcaggcatta tggggcagtt gccggatgcg
    11581 tcatcacatc cggcaaacac acatcacgga tgagttacaa accgggaaag tcgctggcgc
    11641 agcaggcggt tttcctgctt caggtgcgca atatcatcca ttaacgtcag cgccaccgcg
    11701 atcccaggcc agtccagagc cagttcatga cgcaggcgta ctgcgcgttg caccacgatg
    11761 gcggcatggt cgtcaaatac ccaggttgtt tcctggatct cacgcggttc aaccaccccc
    11821 aaaccgacaa tttcattcaa ctcctcttca gagatgccgg tatgcaggca aaattcggta
    11881 atagtaaaag tcaccgtaac attagccatt atgctttccc ccaatcttta cgtggatcaa
    11941 aagacgactg ggcgtctgcc agttgctgcc acagcgcggc agtgttttca tccggtttcg
    12001 gcggcatcac gattttcagt accgcataca gatcgccggt ctgttttttg ctcaccagac
    12061 ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc tggcgggata gtcagcaaaa
    12121 tgctttcttt cagtgttgga acggtgactt tagcacccag cgccgcttcc cacgggctaa
    12181 ccggcactac aatttccaga tcatggccga caatatcgaa cagcggatgt ggcgcaatat
    12241 gaatcaccag ccacaaatcg ccatttggac cgccgttttc gcccggcgtc ccctggcctt
    12301 tcagacggat gcgttgacca ttgccgacgc ccgccgggat cttgacattc agcgttttcg
    12361 gaatttcctg ttcgatcatg ccaaaggcgt tataaaccgg caggttatag ctgatggtac
    12421 gcttatgctc agtaagcgtt tcttcgagga ataccgccac ttcgatttca atatcgtggc
    12481 cgcgtgtggc ggggcgttga cggctctggc gggcatgctg accgaaaatt gacgagaaga
    12541 tatcgtcaaa atcttcggcg ttaaaactct gaccgtcgct atgatggaac tgacggttaa
    12601 attgcggatc gttgcgatgt tgccacatct gatcatactc agcgcgacgt tgttcatcac
    12661 ttaacacttc ccaggcttca gcgacctctt tgaagcgggc ttcggcatcc ggttctttgc
    12721 tgacatcagg atggtatttg cgggccagtc gacgataggc ggtcttgatt gtcttgagat
    12781 cgtccgtcgg tttcacgccc atgatggcgt aataatcctt taattccata gcgttatctc
    12841 gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa ctcctataag tgtagggtaa
    12901 tcctcaaaat ttcatatgcc aacacagaat atgttattga aatcatcgcg gagaggaggt
    12961 cgcctcaagg tggggtgctg aacatatttt aaacaggtga aaaagggtga gcgatttttg
    13021 atagtggaac caggcacttt aagtttaact ggggcgtcat tatttattaa attttatagg
    13081 cgctatat
//
LOCUS       DAZFRJ010000127        13046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000127 DAZFRJ010000000
VERSION     DAZFRJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.128"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..1214
                     /gene="livK"
                     /locus_tag="ACHKUN_003277"
     CDS             105..1214
                     /gene="livK"
                     /locus_tag="ACHKUN_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO6661487.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1262..2188
                     /gene="livH"
                     /locus_tag="ACHKUN_003278"
     CDS             1262..2188
                     /gene="livH"
                     /locus_tag="ACHKUN_003278"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO6661488.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2185..3462
                     /gene="livM"
                     /locus_tag="ACHKUN_003279"
     CDS             2185..3462
                     /gene="livM"
                     /locus_tag="ACHKUN_003279"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO6661489.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3459..4226
                     /gene="livG"
                     /locus_tag="ACHKUN_003280"
     CDS             3459..4226
                     /gene="livG"
                     /locus_tag="ACHKUN_003280"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO6661490.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4228..4941
                     /gene="livF"
                     /locus_tag="ACHKUN_003281"
     CDS             4228..4941
                     /gene="livF"
                     /locus_tag="ACHKUN_003281"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO6661491.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5337..6653
                     /gene="ugpB"
                     /locus_tag="ACHKUN_003282"
     CDS             5337..6653
                     /gene="ugpB"
                     /locus_tag="ACHKUN_003282"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO6661492.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6751..7638
                     /gene="ugpA"
                     /locus_tag="ACHKUN_003283"
     CDS             6751..7638
                     /gene="ugpA"
                     /locus_tag="ACHKUN_003283"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO6661493.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7635..8480
                     /gene="ugpE"
                     /locus_tag="ACHKUN_003284"
     CDS             7635..8480
                     /gene="ugpE"
                     /locus_tag="ACHKUN_003284"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO6661494.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8482..9552
                     /gene="ugpC"
                     /locus_tag="ACHKUN_003285"
     CDS             8482..9552
                     /gene="ugpC"
                     /locus_tag="ACHKUN_003285"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO6661495.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9549..10292
                     /gene="ugpQ"
                     /locus_tag="ACHKUN_003286"
     CDS             9549..10292
                     /gene="ugpQ"
                     /locus_tag="ACHKUN_003286"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6661496.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10279..10719)
                     /locus_tag="ACHKUN_003287"
     CDS             complement(10279..10719)
                     /locus_tag="ACHKUN_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO6661497.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10839..12581
                     /gene="ggt"
                     /locus_tag="ACHKUN_003288"
     CDS             10839..12581
                     /gene="ggt"
                     /locus_tag="ACHKUN_003288"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO6661498.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12619..12903)
                     /locus_tag="ACHKUN_003289"
     CDS             complement(12619..12903)
                     /locus_tag="ACHKUN_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO6661499.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2892 a         3325 c         3748 g         3081 t
ORIGIN      
        1 cttaatcaga caggcaaaaa cagtgcagta taaaaaaaga acagtctgat ttgttaacac
       61 ataaaaacaa agcaacacaa catcacgaat ggggattttt gactatgaaa cggaatgcga
      121 aaactatcat cgcagggatg attgcactgg caatttcaca caccgctatg gctgacgata
      181 ttaaagtcgc cgttgtcggc gcgatgtccg gcccgattgc ccagtggggc gatatggaat
      241 ttaacggcgc gcgtcaggcg attaaagaca ttaatgccaa agggggaatt aagggcgaca
      301 agctggttgg cgtggaatat gacgacgcct gcgacccgaa acaagccgtt gcggtcgcca
      361 acaaaatcgt taatgacggc attaaatacg ttattggtca tctgtgttct tcttctaccc
      421 agcctgcgtc agatatctat gaagacgaag gtattctgat gatctcgccg ggagcgacca
      481 acccggagct gacccaacgc ggttatcaac acattatgcg tactgccggg ctggactctt
      541 cccaggggcc aacggcggca aaatacattc ttgagacggt gaagccccag cgcatcgcca
      601 tcattcacga caaacaacaa tatggcgaag ggctggcacg ttcggtgcag gacgggctga
      661 aagctgctaa cgccaatgtt gttttcttcg acggcattac cgctggcgag aaagatttct
      721 ccgcgctgat cgcccgcctg aaaaaagaaa acatcgactt cgtttactac ggcggttact
      781 acccggaaat ggggcagatg ctgcgccagg cccgttccgt tggcctgaaa acccagttta
      841 tggggccgga aggtgtgggt aatgcgtcgc tgtcgaatat tgctggcgat gctgccgaag
      901 gcatgttggt caccatgcca aaacgctatg accaggatcc ggcaaaccag ggcatcgttg
      961 atgcgctgaa agcagacaag aaagatccgt ccgggcctta tgtctggatc acttacgcgg
     1021 cggtgcaatc tctggcaaca gcccttgagc gtaccggcag cgatgagccg ctggcgctgg
     1081 tgaaagattt aaaagctaac ggtgcaaaca ccgtgattgg gccgctgaac tgggatgaaa
     1141 aaggcgatct taagggattt gattttggtg tcttccagtg gcacgccgac ggttcatcca
     1201 cggcagccaa gtgatcatcc caccgcccgt aaaatgcggg cgggtttaga aaggttacct
     1261 tatgtctgag cagtttttgt atttcttgca gcagatgttt aacggcgtca cgctgggcag
     1321 tacctacgcg ctgatagcca tcggctacac catggtttac ggcattatcg gcatgataaa
     1381 cttcgcccac ggcgaggttt atatgattgg cagctacgtc tcatttatga tcatcgccgc
     1441 gctgatgatg atgggcattg ataccggctg gctgctggtg gcggcgggat tcgtcggcgc
     1501 aatcgtcatt gccagcgcct acggctggag tatcgaacgg gtggcctatc gcccggtgcg
     1561 taactctaag cgcctgattg cgctcatctc ggcaatcggt atgtccatct tcctgcaaaa
     1621 ctacgtcagc ctgaccgaag gttcgcgtga cgtggcgctg ccgagcctgt ttaacggtca
     1681 gtgggtggtg ggccatagcg aaaacttctc tgcctctatt actaccatgc aggcggtgat
     1741 ctggattgtc accttcctcg ccatgctggc gctgacgatt ttcattcgct attcccgtat
     1801 ggggcgcgcg tgtcgtgcct gcgcggaaga tctgaaaatg gcgagtctgc ttggcattaa
     1861 caccgaccgg gtgattgcgc tgacctttgt gattggcgcg gcgatggcgg cggtggcggg
     1921 tgtgctgctc ggtcagttct acggcgtaat taacccctac atcggcttta tggccgggat
     1981 gaaagccttt accgctgcgg tgctcggtgg gattggcagc attccagggg cgatgattgg
     2041 tgggctgatc ctggggattg cggaggcgct ctcttctgcc tatctgagta cggaatataa
     2101 ggatgtggtt tcatttgccc tgctgattct ggtgctgctg gtgatgccga ccggtattct
     2161 gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata ttgcaatggc gctgctctct
     2221 gccgcgatgt tttttgtcct ggcgggcgtc tttatgggcg tgcaactgga gctggatggc
     2281 accaaactgg tggtcgacac ggcttcagac atccgttggc agtgggtgtt tatcggcacg
     2341 gcggtggtct ttttcttcca gcttttgcga ccggctttcc agaaagggct aaaaagcgtt
     2401 tccggaccga agtttattct gcccgcgatt gatggctcca cggtgaagca gaaactattc
     2461 ctcgtggcgc tgctggtgct tgcggtggcg tggccgttta tggtgtcgcg tgggacggtg
     2521 gatatcgcca ccctgaccat gatctacatt atcctcggcc tcgggctgaa cgtggttgtt
     2581 ggtctttctg gtctgctggt gctggggtac ggcggctttt acgccatcgg cgcttacact
     2641 tttgcgctgc tcaatcacta ttacggcctg ggcttctgga cctgtctgcc gattgccggg
     2701 ttaatggcag cggcggccgg cttcctgctc ggcttcccgg tgcttcgctt gcgcggcgac
     2761 tatctggcga tcgttaccct cggtttcggc gaaattgtgc gcatattgct gctcaataac
     2821 accgaaatta ccggcggccc gaacggaatc agtcagatcc cgaaaccgac actcttcgga
     2881 ctcgagttca gccgtaccgc tcgtgaaggc ggctgggaca cgttcagtaa tttctttggc
     2941 ctgaaatacg atccctccga tcgtgtcatc ttcctctacc tggtggcgtt gctgctggtg
     3001 gtgctaagcc tgtttgtcat taaccgcctg ctgcggatgc cgctggggcg tgcgtgggaa
     3061 gcgttgcgtg aagacgagat cgcctgccgt tcgctgggct taagcccgcg tcgtatcaag
     3121 ctgactgcct ttaccataag tgccgcgttt gccggttttg ccggaacgct gtttgctgcg
     3181 cgccagggct ttgtcagccc ggaatccttc acctttgctg aatcggcgtt tgtactggcg
     3241 atagtggtgc tcggcggtat gggatcgcag tttgcggtga ttctggcggc aattttgctg
     3301 gtggtatcgc gcgaattgat gcgtgatttc aacgaataca gcatgttaat gctcggtggt
     3361 ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc tgcccatgac gcgcccgcaa
     3421 ctgaagctga aaaacggcgc agcgaaagga gagcaggcat gagtcagcca ttattatctg
     3481 ttaacggtct gatgatgcgc ttcggcggct tgctggcggt gaacaacgtc aatcttgaac
     3541 tgtacccgca ggagatcgtc tcgttaatcg gccctaacgg tgccggaaaa accacggttt
     3601 ttaactgcct gaccggattc tacaaaccca ccggcggcac tattttactg cgtgatcagc
     3661 atctggaagg tttaccgggg cagcaaattg cccgcatggg cgtggtgcgt accttccagc
     3721 atgtgcgtct gttccgtgaa atgacggtga ttgaaaacct gctggtggcg cagcatcaac
     3781 aactgaaaac cgggctgttc tctggcctgt tgaaaacgcc ttctttccgt cgcgcccaga
     3841 gcgaagcgct cgaccgcgcc gccacctggc ttgagcgcat tggtttgctg gaacacgcca
     3901 accgtcaggc gagtaacctg gcctatggtg accaacgccg tctggagatt gcccgctgca
     3961 tggtgacgca gccggagatt ttaatgctcg acgaaccggc ggcaggtctt aacccaaaag
     4021 aaaccaaaga gctggatgag ctgattgccg aactgcgcaa tcatcacaac accactatct
     4081 tgttgattga acacgatatg aagctggtga tggggatttc agaccgaatt tatgtggtca
     4141 atcaggggac gccactggca aacggtacgc cggagcaaat ccgtaataac ccggacgtga
     4201 tccgtgccta tttaggtgag gcataagatg gaaaaagtca tgttgtcctt tgacaaagtc
     4261 agcgcccact acggcaaaat ccaggcgctg catgaggtga gcctgcatat caatcagggc
     4321 gagattgtca ccctcattgg cgcgaacggg gcggggaaaa ccaccttgct cggcacgtta
     4381 tgcggcgatc cgcgcgctac cagcgggcga attgtgtttg atgataaaga cattaccgac
     4441 tggcagacgg cgaaaatcat gcgcgaagcg gtagcgattg tcccggaagg gcgtcgcgtc
     4501 ttctcgcgga tgacggtgga agagaacctg gcgatgggcg gcttttttgc cgaacgcgac
     4561 cagttccagg agcgcataaa gtgggtgtat gaactgtttc cacgtctgca tgagcgccgt
     4621 gttcagcggg caggcaccat gtccggcggt gagcaacaaa tgctggcgat tggtcgcgcg
     4681 ctgatgagca acccgcgttt gctgctactt gatgagccat cgctcggtct tgcgccgatt
     4741 atcatccagc aaattttcga caccatcgag cagctgcgcg agcaggggat gactatcttc
     4801 ctcgtcgagc agaacgccaa ccaggcgcta aagctggcgg atcgcggcta cgtgctggaa
     4861 aacggccatg tggtgctctc cgataccggc gacgcgctgc tggcgaacga agcagtgaga
     4921 agtgcgtatt taggcgggta ataacacgtt gattgataag gagtcaaaag actcctttga
     4981 gacaggtgac aaatgtaaaa ttgcctgatg cgctgcgctt atcaggccta ctgggtgagt
     5041 tgcaatatat tgaatttgca cgatcttgta ggccggataa ggcgtttacg ccgcatccgg
     5101 catgaaacga tgggtaatct gtagagtttg attcagacct tctattttcc gcttatccgt
     5161 gccccatctc ccattttccc tcacccacgc cgtcaccgcc ttgtcatctt tctgacacct
     5221 tactatctta caaatgtaac aaaaaagtta tttttctgta attcgagcat gtcatgttac
     5281 cccgcgagca taaaacgcgt gaattcgcgc attcggtaca acaagagaga taaacgatga
     5341 aaccgttaca ttatacagct tcagcactgg cgctcggact ggcgttaatg gggaatgcac
     5401 aggcagtgac gaccattccg ttctggcatt ctatggaagg ggaactgggt aaagaggtgg
     5461 attctctggc ccaacgtttt aacgccgaaa atccggatta caaaattgta ccgacctata
     5521 aaggcaacta cgaacagaat ttaagcgcgg ggattgccgc atttcgtacc ggcaacgctc
     5581 cggctatttt gcaggtttat gaagttggca ccgccaccat gatggcatcg aaagccatta
     5641 aaccggtata tgacgtgttt aaagaggcgg ggattcaatt cgatgagtcg cagtttgtgc
     5701 cgacggtttc cggttactac tccgacagca aaacgggcca cttactctcc cagccattca
     5761 acagctcgac ccccgttctc tattacaaca aagacgcctt caagaaagca ggattagacc
     5821 cggaacaacc gccgaaaacc tggcaggatc tggcggacta tgccgcgaaa ctgaaagcct
     5881 ccggtatgaa gtgcggctac gccagcggct ggcagggctg gatccaactg gaaaacttta
     5941 gcgcctggaa cggtctgccg tttgccagca aaaacaacgg ctttgacggc acggacgcgg
     6001 tgctggagtt caacaagccg gagcaggtga aacatatcgc catgctcgag gagatgaaca
     6061 agaagggcga cttcagctac gtcggtcgta aggatgaatc caccgagaag ttctataacg
     6121 gtgattgcgc gatgaccacc gcctcttccg gttctcttgc caacattcgc gagtacgcca
     6181 aatttaatta cggcgtaggc atgatgcctt acgacgccga tgcgaaagat gcgccacaaa
     6241 acgccattat cggcggagcc agcctgtggg tgatgcaggg taaagataaa gaaacgtata
     6301 ccggtgtggc gaagttcctc gatttcctcg cgaagccaga aaacgctgcc gagtggcatc
     6361 agaaaaccgg ttatctgcca attaccaaag cggcgtatga cctgacccgt gagcagggct
     6421 tttacgagaa aaacccaggt gcggataccg cgacgcgtca gatgctgaat aagccgccgt
     6481 tgccgttcac caaagggctg cgtctgggca acatgccgca gatccgcgtg attgtggatg
     6541 aagagctgga gagcgtgtgg accggtaaga agacaccaca gcaggcactg gataccgccg
     6601 ttgagcgtgg aaatcagttg ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt
     6661 aatgtcggat gcgtttcgct tatctgactt ggcatcgcgt gtaggccgga taagcgaagc
     6721 gcatccggca cagttcagga attaaccgta atgtcatcat cccgtccggt gttccgctcg
     6781 cgctggctgc cttatctgct ggtcgcgccg cagctcatca tcaccgttat cttttttatc
     6841 tggcctgcgg gcgaagcgtt gtggtactcg ctacaaagcg tcgatccgtt tggtttctcc
     6901 agccagtttg tcggcctgga taacttcgtc gcgctgtttc atgacagcta ctatctcgac
     6961 tccttctgga cgacgataaa attcagcacc tttgtcaccg tcagcggttt gctggtgtcg
     7021 ctgttcttcg cggcgctggt ggagtacatc gtgcgcggca gccgtttcta tcaaacctta
     7081 atgctgctgc cttacgccgt ggctcccgcc gttgccgccg tattgtggat cttcctgttt
     7141 aaccccggtc gcgggctgat cacccatttt ctcgcggagt tcggctacga ctggaaccac
     7201 gcgcaaaaca gcggtcaggc aatgtttctg gtggtgtttg cctcagtatg gaagcaaatc
     7261 agctacaact tcctgttctt ctatgccgcg ctgcaatcca ttccccgttc gttgatcgaa
     7321 gccgcagcca tcgacggtgc aggcccaatt cgccgcttct ttaagattgc gctgccgctt
     7381 atcgctccgg tgagtttctt cctgctggtg gtgaacctgg tgtatgcctt cttcgacacc
     7441 ttcccggtga tcgacgccgc cacgtccggc gggccggttc aggccaccac gacgctgatt
     7501 tataagatct accgtgaagg ctttaccgga ctggatctgg cttcgtctgc cgcgcagtcg
     7561 gtggtgttga tgttcctcgt catcgtgctg acggtggtgc agttccgcta tgttgaaagc
     7621 aaggtgcgtt accaatgatt gagaaccgtc cgtggctgac gatattcagc cataccatgc
     7681 tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt ggcgtttgtc gcggcgacgc
     7741 tggataaaca ggccgtctat gccgcgccga tgacgctcat ccccggcaca catctgctgg
     7801 aaaatatcca caacatctgg gttaacgggg taggcacgaa tagcgcgccg ttctggcgga
     7861 tgttgcttaa cagctttgtg atggcgttca gcattacgct cggcaaaatt accgtctcga
     7921 tgctctcggc atttgccatt gtctggtttc gttttccgct acgtaacctc ttcttctgga
     7981 tgatttttat caccctgatg ctgccggttg aagtacgtat cttcccgacg gtggaagtca
     8041 tcgccaacct gaagatgctc gacagctacg ccggtttaac gctgccgctg atggcctcgg
     8101 cgaccgccac gtttctgttt cgccagttct ttatgacgct gccggatgaa ctggtggaag
     8161 cggcgcggat cgacggcgcg tcaccaatgc gtttcttttg cgacatcgtt tttccgctct
     8221 ccaaaaccaa tctggcggcg ctgtttgtga tcacctttat ttacggctgg aaccagtatt
     8281 tgtggccgtt gttgattatt accgatgtgg atctcggcac caccgtggca gggatcaaag
     8341 ggatgatcgc tacaggcgaa ggcaccactg aatggaactc agtgatggcg gcgatgttgt
     8401 taacgcttat ccctccggtg gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc
     8461 tggtcgatag tgagaaataa gatggcagga ctgaaattac aggcagtaac caaaagctgg
     8521 gatggtaaaa cccaggtcat taaaccgctg acccttgatg tggcggatgg cgaatttatc
     8581 gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgctgc tgcgcatggt tgccgggctg
     8641 gagcgggtga cagaaggcga tatctggatc aacgaccagc gcgtgactga aatggagcca
     8701 aaagatcgcg ggattgcgat ggtgttccag aactacgcgc tttatccgca tatgagtgtc
     8761 gaagaaaaca tggcgtgggg gctgaaaatt cgcggcatgg gcaagcagca aattgccgag
     8821 cgcgttaaag aagcggcgcg cattctggag ctggacggtc tgctcaaacg tcgcccgcgc
     8881 gagctttccg gcggtcagcg ccagcgtgtg gcgatgggcc gcgcgattgt gcgcgatccg
     8941 gcggtgttcc tgtttgatga gccgctctct aacctcgatg ccaagctgcg cgtgcagatg
     9001 cgtcttgaac tgcaacagtt gcaccgtcgc ctgaaaacga cttcactcta cgttactcac
     9061 gatcaggttg aagcgatgac gctcgcccag cgagtaatgg tgatgaacgg cggcgttgcc
     9121 gaacagattg gcacaccagt tgaagtctac gaaaagcccg ccagcctgtt tgtagcgagt
     9181 tttatcggca gtccggcgat gaacctgctg acaggccgcg tgaataacga aggcacgcat
     9241 ttcgaactgg acggcggtat tgagctgccg ctaaacggtg gctaccgtca gtatgccggg
     9301 cgtaaaatga ctctcggcat tcgcccggaa catattgcgc taagctcgca ggcagaaggc
     9361 ggcgtaccgc tggtgatgga cacgctggag atcctcggcg cagataacct ggcgcacgga
     9421 cgctggggcg agcagaagct ggtggtgcga ctggcgcatc aggagcgccc gacggcaggc
     9481 agcacgctgt ggctgcatct ggcggaaaat cagctgcatc tttttgatgg tgaaacagga
     9541 caacgagtat gagtaactgg ccttatcccc gcatcgtcgc tcatcgtggc ggcggtaagc
     9601 tggccccgga aaacaccctg gcggcaatcg acgtcggggc aaaatacggt cataagatga
     9661 tcgaatttga cgcgaagtta tcgaaagatg gcgagatctt cctgctccat gacgacaatc
     9721 tcgaacgcac cagcaacggc tggggcgtcg cgggtgaact gaactggcag gatttactgc
     9781 gcgtggatgc gggcagttgg tacagcaaag cgtttaaagg tgagccgctg ccgttgcttt
     9841 cgcaggtggc ggaacgctgt cgcgaacacg ggatgatggc gaatatcgaa atcaaaccca
     9901 ccactggcac tggaccatta acgggcaaaa tggtggcgct ggcggcacgc caactgtggg
     9961 ccggtatgac gccgccgctg ctgtcatcgt ttgagattga tgctttagaa gctgcacagc
    10021 tggcggcacc ggaactgccg cgtggtttat tgcttgatga gtggcgcgac gactggcgcg
    10081 aactgaccgc gcggctgggc tgcgtctcta ttcatctcaa tcataagtta ctcgataaag
    10141 tgcgagtgat gcagttgaaa gacgccggac tgtggattct ggtttatacc gtcaataaac
    10201 cccagcgcgc ggcagagttg ctgcgctggg gcgtggattg catctgtacc gatgcgattg
    10261 acgtgattgg tccgaacttt acggcccaat agttttcaac ggaatgtcag gctgcggcgt
    10321 gctgggctga ttcagcatgt cgccgtttct cctctccggc agcatatgct gctgattcaa
    10381 cgaactatcc ggattacggt tgctgtttaa catcccgccg ttggtgttgg gcagcatttg
    10441 ctgccgggcg ggatttcgct cccccggctg tgactgcaac acccgctgag aattattgtt
    10501 tatctggttt tctaaatgct gctgttgcaa ctgagtttgc gttttcagtt gctgattcag
    10561 catccctttt tgctggattt gctgagtctg catctgggtt tgcatccgct gctggctggg
    10621 tatctgatac cccggctggt tagggttgtt cagagtatta atgggctgtg caaagccgac
    10681 aaacggcagg agtgccgtaa gaatcagaag ccgtttcatc gcgtatcctc ctctgaagat
    10741 atcctttaag tttactcgct tcccgacaaa acgatgatta attcagagtt atataccagg
    10801 cttagctggg gttgcccctt aatctctgga gaataacgat gataaaaccg acgtttttac
    10861 gccgggtggc cattgctgct ctgctctcag gaagttgttt tagcgccgcc gccgcgcctc
    10921 ctgcgccgcc cgtctcgtat ggtgtggagg aagatgtctt ccacccggta cgcgcgaaac
    10981 agggaatggt agcgtctgtg gacgccactg ccactcaggt gggggtggat attctcaagg
    11041 agggcgggaa tgccgttgat gccgccgtgg cggtgggcta cgcgctggcg gtaacgcatc
    11101 cgcaggcagg gaatctgggc ggtggtggtt ttatgctgat ccgctcgaaa aatggcaata
    11161 ccaccgctat cgatttccgc gaaatggcac ccgctaaagc gacccgcgat atgttccttg
    11221 atgaccaggg caacccggac agcaaaaaat cactcacttc gcatctggct tccggcacac
    11281 caggtacggt cgctggtttc tcgctggcgt tggataagta cggcactatg ccgttgaaca
    11341 aagtggtgca gcccgcgttc aaactggcac gcgatggttt tatcgttaac gatgctttgg
    11401 cagacgatct caaaacctac ggtagcgaag tgttgccgaa tcacgaaaac agcaaagcta
    11461 tcttctggaa agagggcgaa ccgctgaaaa agggcgacac gctggtgcag gcgaacctgg
    11521 caaagagcct ggagatgatt gctgaaaacg gcccggacga attctataaa ggcacgattg
    11581 cggaacagat cgcccaggag atgcagaaaa acggtggctt gatcactaaa gaagatttag
    11641 cggcctataa agcggtcgaa cgcactccga taagcggcga ttatcgcggg tatcaggttt
    11701 actccatgcc accgccatcc tccggcggga tccatatcgt acaaatcctc aatattctgg
    11761 aaaacttcga tatgaagaaa tacggctttg gcagcgccga tgcgatgcaa atcatggcag
    11821 aagcggagaa atacgcctac gccgaccgct cggaatatct tggcgacccg gattttgtca
    11881 aagtcccgtg gcaggcactg accaataaag cctatgccaa atccattgcc gatcaaattg
    11941 atatcaacaa agcgaagccg tccagtgaga ttcgccctgg caagcttgcg ccttatgaga
    12001 gtaatcaaac tacccattac tctgtagtgg ataaagacgg taacgcggtg gcggtgacct
    12061 atacgctgaa caccaccttc ggtacgggta ttgtcgcggg cgagagcggt attctgctta
    12121 ataaccagat ggatgatttc tccgccaaac cgggcgtacc gaacgtttac gggctggtgg
    12181 gcggtgatgc caacgccgtc gggccgaaca aacgcccgct gtcgtcgatg tcgccgacca
    12241 ttgtggtgaa agacggtaaa acctggctgg ttaccggtag cccaggcggt agccggatca
    12301 tcactacagt gctgcaaatg gtggtgaata gcatcgatta tggcatgaac gtcgccgaag
    12361 cgaccaatgc gccgcgtttc caccatcagt ggttgccgga cgagctgcgt gtcgaaaaag
    12421 ggtttagccc ggatacgctc aagctgctgg aagcaaaagg tcagaaagtg gcgctgaaag
    12481 aggcgatggg cagtacacaa agcattatgg ttgggccgga cggtgagttg tacggcgcat
    12541 ccgacccgcg ctcggtggat gatttaacgg cggggtacta aggttagcgg ccctcttcgt
    12601 gggaagaggg ctattttgtc agggcaagcc gaaggtagcc ttttttattt cgtaatcctg
    12661 tagatattct tccagcggct ttgccgttcc cagagaatga atttcaccac tgtctttatc
    12721 aataataaaa ggtgcgttac cagctaagcg cgcggcctca tctccagttt cgagaaattc
    12781 tcgtgcttcg aaacagaaat accagccctg gctaaagcgt ccatgtagag taatgacgac
    12841 cgggagatct gcatcatcaa ggtaatggtt cgctttcgcg aatgcgtcgt gataagtaat
    12901 cataattata ataaatatta ttgttgagtg ttatattatt attcctgtgt gatacattga
    12961 gcaaagacgc gttcatcttc gtcaaagata ttattaaatg ctggttttga aaaaagttca
    13021 tcggataaga tatagtcacg aaataa
//
LOCUS       DAZFRJ010000128        12948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000128 DAZFRJ010000000
VERSION     DAZFRJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.129"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            292..873
                     /gene="mdaB"
                     /locus_tag="ACHKUN_003290"
     CDS             292..873
                     /gene="mdaB"
                     /locus_tag="ACHKUN_003290"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO6661500.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            904..1218
                     /locus_tag="ACHKUN_003291"
     CDS             904..1218
                     /locus_tag="ACHKUN_003291"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO6661501.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1266..3158)
                     /gene="parE"
                     /locus_tag="ACHKUN_003292"
     CDS             complement(1266..3158)
                     /gene="parE"
                     /locus_tag="ACHKUN_003292"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO6661502.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3187..3768)
                     /gene="yqiA"
                     /locus_tag="ACHKUN_003293"
     CDS             complement(3187..3768)
                     /gene="yqiA"
                     /locus_tag="ACHKUN_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO6661503.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3768..4595)
                     /gene="cpdA"
                     /locus_tag="ACHKUN_003294"
     CDS             complement(3768..4595)
                     /gene="cpdA"
                     /locus_tag="ACHKUN_003294"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO6661504.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4620..5042)
                     /locus_tag="ACHKUN_003295"
     CDS             complement(4620..5042)
                     /locus_tag="ACHKUN_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO6661505.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5043..5672)
                     /gene="nudF"
                     /locus_tag="ACHKUN_003296"
     CDS             complement(5043..5672)
                     /gene="nudF"
                     /locus_tag="ACHKUN_003296"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO6661506.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5877..7358
                     /gene="tolC"
                     /locus_tag="ACHKUN_003297"
     CDS             5877..7358
                     /gene="tolC"
                     /locus_tag="ACHKUN_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO6661507.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7506..8177
                     /locus_tag="ACHKUN_003298"
     CDS             7506..8177
                     /locus_tag="ACHKUN_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO6661508.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8183..9343
                     /locus_tag="ACHKUN_003299"
     CDS             8183..9343
                     /locus_tag="ACHKUN_003299"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6661509.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9381..10169)
                     /gene="ygiD"
                     /locus_tag="ACHKUN_003300"
     CDS             complement(9381..10169)
                     /gene="ygiD"
                     /locus_tag="ACHKUN_003300"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO6661510.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10312..11085
                     /gene="zupT"
                     /locus_tag="ACHKUN_003301"
     CDS             10312..11085
                     /gene="zupT"
                     /locus_tag="ACHKUN_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO6661511.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11143..11313)
                     /gene="yqiD"
                     /locus_tag="ACHKUN_003302"
     CDS             complement(11143..11313)
                     /gene="yqiD"
                     /locus_tag="ACHKUN_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO6661512.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11574..12227)
                     /gene="ribB"
                     /locus_tag="ACHKUN_003303"
     CDS             complement(11574..12227)
                     /gene="ribB"
                     /locus_tag="ACHKUN_003303"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO6661513.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12331..12479)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12601..12891
                     /gene="ubiK"
                     /locus_tag="ACHKUN_003304"
     CDS             12601..12891
                     /gene="ubiK"
                     /locus_tag="ACHKUN_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO6661514.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3271 a         3460 c         3168 g         3049 t
ORIGIN      
        1 tataattatc aacatgacac gttgatattt tcatctttta cttattcaaa gccatatttt
       61 caatcccaaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta agaattatga
      121 ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg tgagtcagag
      181 caagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa tttcaccgta
      241 ccgctctgtg acgtactata gtcggcaaac gtctcacctt gaggttaaaa aatgagcaac
      301 atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc
      361 ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg catcgttcgc
      421 gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg
      481 atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa atatattgat
      541 gatgtattca ccgaaggtca tggaacgctg tatgccagcg atggccgtac ccgcaaagat
      601 ccgtcaaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat gctttctctg
      661 acctggaacg caccaatgga agccttcacc gaaaaagatc aattcttcca cggcgttggc
      721 gtcgacggtg tgtatctgcc gttccataaa gcaaaccaat tccttggcat ggaaccgctg
      781 ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa
      841 tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta gaaggagtta
      901 accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg
      961 gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacggc
     1021 tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct
     1081 atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct gcaaaccccg
     1141 cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa tatccgcatc
     1201 ctgcaacctg ggatttaaac ctgccttgtt tgcccggcca tcctgaccgg gcaatgttct
     1261 ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt gcggcgatct
     1321 tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca
     1381 tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt ttcacgcaat
     1441 tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt gcctttcttg
     1501 cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct
     1561 ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc
     1621 aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg cagaccatca
     1681 gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag atcgtcgctg
     1741 tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc cagcacttcg
     1801 tcggaagaaa cttcccaggt gttaaggatc ttacctttca gtggcatgat cgcctgatat
     1861 tcgcgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac aaggaacagt
     1921 tcggtacggt taagatcctg cgcggtacag tcagccagtt tgccaggcag tgctgggccg
     1981 ctggtcagct ttttgcgcac cactttttta gccgcacgca tacggcgctg ggcgctggaa
     2041 atcgccatct ccgccagcag ctccgccgcc tgaacgttct ggttcagcca caggataaag
     2101 gcatctttca ccacgcctga aacgaatgcc gcgcattgac gcgaagagag acgctctttg
     2161 gtctgcccgg caaactgcgg atcctgcatt ttcaccgaca gaacataggc gcagcgatcc
     2221 cagatatctt ccgccgacag cttcacgccg cgcggcagaa tgttgcggta ttcacagaac
     2281 tcacgcatcg cgtccaacag gccctgacgc agaccattaa catgggtacc gccctgcatc
     2341 gttgggataa ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt
     2401 agcgcccagt ccacagcttc agtatcgcca gcgaaattac cgataaacgg tttttctggc
     2461 agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac
     2521 cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt
     2581 actgctttgg ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctgtcaaag
     2641 aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg
     2701 acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata aacctgacca
     2761 tcgcggcgca cgttaacttc tacgcgcttc gacagggcgt taaccaccga aatccccacg
     2821 ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc tgcatgcaga
     2881 cggcaaagaa tcagttcaac cgccggtaca ccctcttccg ggtgaatatc caccggcatc
     2941 ccgcgcccat cgtcaataac ttctaacgac tggtcagcat gtaagattac atccacgcgt
     3001 tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg
     3061 ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg
     3121 agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt
     3181 tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa
     3241 gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag cgtagtacgc
     3301 caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc
     3361 cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc gtgactctag
     3421 cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcagttc
     3481 aaacgggcgc accgccgggt ttaccaccac tgcgggcagc ataaaacatt gtgacaacca
     3541 ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc
     3601 caggacaatg gattccagca gctctgccgc gtcggaagga tacggcggca actgcggaat
     3661 gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc
     3721 agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct
     3781 gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg
     3841 ccatcagcat gtaactcgag agtacgccag ccgggcgcga tggtgtccag cgtaaagttg
     3901 gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg gcgaccgttc
     3961 cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac gtgcggaaac
     4021 ttcgccagca cggtatccag ttcgcccgcg ttacgcaggc tgtgttgatc aagccaacta
     4081 caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc tggcgcatcg
     4141 gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg cggcacgcca
     4201 aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac ccgcttcgcc
     4261 ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc gtggttgcca
     4321 ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ccgataggcc
     4381 gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg
     4441 tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtatttac ccctaacagg
     4501 gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct gactctggcc
     4561 tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc
     4621 tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa
     4681 aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac
     4741 cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga
     4801 tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct
     4861 attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt
     4921 gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat
     4981 tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc
     5041 atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg
     5101 accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt
     5161 accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc gcttgcggtc
     5221 gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc cgggcttgcc
     5281 aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc aatcgcttcg
     5341 cgacgggcaa catcttccac actttcaccc tcttctatca tcccggcaac catctccagt
     5401 agccaggggg tttcgctggt gtcgtacgcg gcaatccgaa tctgctcaat cagcacaact
     5461 tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc
     5521 cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct ataaagatct
     5581 aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca
     5641 aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact aatagtgaat
     5701 taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt aaattgaggc
     5761 acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggattc tgctagaatc
     5821 cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga
     5881 agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg ttgagccagg
     5941 ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt
     6001 ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc
     6061 cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca
     6121 tcaactctaa cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga
     6181 aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc acatatcaga
     6241 ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta
     6301 ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa ttagatcaaa
     6361 ccacccaacg ttttaacgtg ggcctggtag cgataaccga cgtgcagaac gcccgcgcgc
     6421 agtacgatac cgtgctggcg aacgaagtga ccgcacgcaa taaccttgat aacgcggtag
     6481 agcagctgcg ccagatcacc ggtaactact atccagaact ggcagcgctg aatgtcgaaa
     6541 actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc gaaaaacgca
     6601 acctgtcgct gttacaggca cgcttgagcc aggacctggc gcgcgagcaa attcgccagg
     6661 cgcaggatgg tcacttaccg acgctggatt taacggcttc taccgggatt tctgacacct
     6721 cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgac agcaatatgg
     6781 gccagaacaa agtgggcctg agcttctcgc tgccgattta tcagggcgga atggttaact
     6841 cgcaggtgaa acaggcacag tacaactttg tcggtgccag cgagcaactg gaaagcgcgc
     6901 atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgca tctatcagta
     6961 gcattaacgc ctacaaacaa gccgtagttt ccgctcaaag ctcattagac gcgatggaag
     7021 cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcaacc accacgctgt
     7081 acaacgctaa gcaagagctg gcaaatgcgc gttataacta cctgattaat cagctgaata
     7141 ttaagtcagc cctgggtacg ttgaacgagc aggatctgct ggcactgaac aatgcgctga
     7201 gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta
     7261 ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca tccgcacgca
     7321 ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg ggcttcggcc
     7381 ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc
     7441 gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga
     7501 caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcgc
     7561 gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa
     7621 agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag
     7681 gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg
     7741 cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgccagc
     7801 aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag caatccagcg
     7861 ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg ggcggcggcg
     7921 cgggatttgc acagcagccg ctgttctcct cgaaaaaccc agccagcccg gcttacggta
     7981 aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc accatgaccg
     8041 taccgaagac agccatggca ccaaaaccgg cgaccaccac taccgttacc cgtggcggtt
     8101 ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaactggc acctcttctc
     8161 gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg
     8221 tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg
     8281 tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc
     8341 cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat
     8401 gaccaaattc cgcattccaa aacacacctg gagttttgtg cgccagtcat ggctgacgca
     8461 ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg aacctaaact
     8521 tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat
     8581 ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag
     8641 tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt
     8701 gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc agtatttgca
     8761 ggactgcgca acggaagctg aaattgctac tgagttcctc tacatcgatg atatcgggtt
     8821 aggtgaaaaa ggtcagttca cggatttaca ggatcaggta atttccaacc tgttcaagct
     8881 gtatccgtgg gaatttatgt tgcgtgagat gttttccacc aagctggagg atgcaggcgt
     8941 acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc taccgctact
     9001 gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca
     9061 tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc
     9121 gatcattgaa aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg aagaagggat
     9181 gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag
     9241 ctggctggtg aacgatcaac ccgccggaat tggcattcgt gaagaccgtg cattgatcac
     9301 ccaggatatg tctcggtttt atccacatat ttttgttgag taatcaatga tgccggatgg
     9361 cattcgccat ccggtaattg ttaacctatc tgcaccgaca gcatactcag gctgcccatt
     9421 tctataccat cgaccggaat ggtaattggc tcctgcccat cccacgcacc taagacatac
     9481 aacaacggca gatagtgctc cggcgttggg ttcgataacg cgccaccttc atggtcgagg
     9541 taattcacca gaggatgctg ctctaccgcc ccttgccacg tcagattcgc tttcacatac
     9601 tcattaaacg acatcgccca aggatatggt gaactatcac cgtgccactt cactgtacgc
     9661 aggttatgca ccacgttgcc actggcgacc aacattattc cttcatctcg cagcgctgcc
     9721 agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat actcaactgc
     9781 accatcggga tatcggcgtc aggatacatc ttaatcagca cgccccagga accgtggtca
     9841 aagccccagg cttctttatc cagcgtcacc gggaccggtg ctaacagctc aaccagatgc
     9901 tgtgccagca caggcgaacc cggagcagga taatgcgtat cgtacagcgc ctgcgggaag
     9961 ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt tccacgggta
    10021 aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat ccccaacttc
    10081 tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc gtgacctaaa
    10141 aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg tgtcattttg
    10201 atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg atgatgatca
    10261 tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc gatgtcagta
    10321 cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt
    10381 ctcgggcaaa aaccctcgaa ccgcttactg gcgttttcgc taggatttgc ggcggggatc
    10441 atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga aggaatgtcg
    10501 cctgtgttgg gttatggaat gtttatcttc ggtctgcttg gctattttgg cctggaccgc
    10561 atgttgccac atgcgcatcc gcaggattta atgcaaaaat cggtgcagcc gttgccaaaa
    10621 tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa cttcccggaa
    10681 gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg catcgcactg
    10741 gccgtcgcgt tgcacaatat tcctgaaggt ctggcggttg caggcccggt ttatgcagca
    10801 acgggttcta aacgcacggc aattctgtgg gcggggattt ctggattggc agaaattctt
    10861 ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt cccccgtggt catggcggca
    10921 atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt aatgccgctc
    10981 gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg aatgtcagtg
    11041 atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaagca acatcagaag
    11101 ttggccagcg catggacaac ttctgctagc ttcactaaag ccttattcta ccttttcggc
    11161 tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc
    11221 ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca caacaagagc
    11281 aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat ttaacaatac
    11341 taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata tggtcgcata
    11401 ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat attaatgaaa
    11461 ttttctcata gaaagagaag cggaattaac atcttgcata gcagggcaaa ataaaaaccg
    11521 aagccagcta caacttcggt ttcttgatta aggcagtaat taagcagcgg ttttcagctg
    11581 gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac gagcgccata
    11641 ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta
    11701 gtcaactcac acagcacacc agccggttta aagcctgcca gcgtcatcaa atcaatagtc
    11761 gcttcagtat gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag tgggaaaacg
    11821 tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc
    11881 gtaattcgat cagcggcaga aacaccggta gtcacacctt cagccgcttc aatggtcacg
    11941 gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag atcaagttgt
    12001 ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg aatggtcagc
    12061 gccatctgct caacggtcat ggtttctgcc gggaagatca tatcgccttc gttttcacgg
    12121 tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag tgcattttca
    12181 acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct
    12241 cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa
    12301 taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg
    12361 tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg
    12421 gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg
    12481 gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata attctggttt
    12541 atgacttacc cttatcgcac tacaatggca ctcaacacct atcagtacag ggaaactaca
    12601 atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa
    12661 ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagctcag
    12721 ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca agtcctgtta
    12781 cggactcgtg agaaactggc gctgcttgaa caaagaatga gcgaactgga aaatcgcagt
    12841 actgagatta aaaagcagcc agatccagag actctccccc caacgctgta aacatttgtc
    12901 tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgcttt
//
LOCUS       DAZFRJ010000129        12839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000129 DAZFRJ010000000
VERSION     DAZFRJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.130"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..752)
                     /locus_tag="ACHKUN_003305"
     CDS             complement(<1..752)
                     /locus_tag="ACHKUN_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6661515.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYL"
     gene            760..1261
                     /gene="tnpA"
                     /locus_tag="ACHKUN_003306"
                     /pseudo
     CDS             760..1261
                     /gene="tnpA"
                     /locus_tag="ACHKUN_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1279..1947)
                     /locus_tag="ACHKUN_003307"
     CDS             complement(1279..1947)
                     /locus_tag="ACHKUN_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO6661516.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2250..2843)
                     /gene="tehB"
                     /locus_tag="ACHKUN_003308"
     CDS             complement(2250..2843)
                     /gene="tehB"
                     /locus_tag="ACHKUN_003308"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO6661517.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2840..3832)
                     /gene="tehA"
                     /locus_tag="ACHKUN_003309"
     CDS             complement(2840..3832)
                     /gene="tehA"
                     /locus_tag="ACHKUN_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO6661518.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3956..4936
                     /gene="ydcK"
                     /locus_tag="ACHKUN_003310"
     CDS             3956..4936
                     /gene="ydcK"
                     /locus_tag="ACHKUN_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO6661519.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4931..5467)
                     /gene="rimL"
                     /locus_tag="ACHKUN_003311"
     CDS             complement(4931..5467)
                     /gene="rimL"
                     /locus_tag="ACHKUN_003311"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO6661520.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5530..5754)
                     /locus_tag="ACHKUN_003312"
     CDS             complement(5530..5754)
                     /locus_tag="ACHKUN_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO6661521.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5894..7549)
                     /gene="opgD"
                     /locus_tag="ACHKUN_003313"
                     /gene_synonym="mdoD"
     CDS             complement(5894..7549)
                     /gene="opgD"
                     /locus_tag="ACHKUN_003313"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO6661522.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7774..9117)
                     /locus_tag="ACHKUN_003314"
     CDS             complement(7774..9117)
                     /locus_tag="ACHKUN_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6661523.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            9334..10257
                     /locus_tag="ACHKUN_003315"
     CDS             9334..10257
                     /locus_tag="ACHKUN_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6661524.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(10295..11935)
                     /gene="trg"
                     /locus_tag="ACHKUN_003316"
     CDS             complement(10295..11935)
                     /gene="trg"
                     /locus_tag="ACHKUN_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO6661525.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            12334..12516
                     /locus_tag="ACHKUN_003317"
     CDS             12334..12516
                     /locus_tag="ACHKUN_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO6661526.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSRLGQARGFSPRI"
     gene            complement(12555..12728)
                     /locus_tag="ACHKUN_003318"
     CDS             complement(12555..12728)
                     /locus_tag="ACHKUN_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661527.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT         3288 a         3211 c         3000 g         3340 t
ORIGIN      
        1 tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat tttgcgtttc
       61 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
      121 agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
      181 gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgagccgga
      241 gtggatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
      301 gtgacatttt tcacaacacc cgtgacctga cggctattcc ggtagcgcat ccagcccagt
      361 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
      421 gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt
      481 ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta
      541 agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa
      601 gggatgtatt tattaccggc ctcatgattc tcattctgac gtgccagagc acgattgaaa
      661 acgaaacgac atgcgcctgc gaagcgcctc atttcacact cctgttgacc acctggtctt
      721 aactggaatt taaatgcctg taatcgtttc acaggtcaaa tgatactttg gtctgtgaat
      781 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
      841 tttgtcacca gataccgacg ccagattttt tgacgcgaca gaaaaactac gcacttactt
      901 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      961 cgtccatttg ttaattaact aacctcccaa actggcgata tccagtctgg taaacagcct
     1021 caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
     1081 ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat
     1141 atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcac
     1201 cttatatccc cgccctgaag gatggggttt tacggcacac cggataaaaa gcgccatcta
     1261 acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat cggtcgccat
     1321 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
     1381 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
     1441 ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca tttgcgtccc
     1501 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
     1561 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccagtgg
     1621 tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa ttttatccag
     1681 aactttctgc cctacctggg tagaaggttt tggcaccgga taataagtga ttgggttcca
     1741 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
     1801 acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
     1861 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
     1921 aactttcgca aatgatgtgg tacgcatgaa tagtttcctg agatgattat attacttttt
     1981 aagttatagc aaaaagcgcc gcttcgtgtt gtggcaaaaa aagtgttggt gaaaataaat
     2041 ttttcagccc cttttcagac gacatagtca atgccgaaaa aggggctaag tcaatcatca
     2101 gtagttaaat ttcagtaagt taagctgcta aaaaacttcg actactgtga ttaatttcac
     2161 atcccttcga taaaaaatat gcttaccgaa aagaagaaca gtaaatcatc tcagcgtggg
     2221 gaattttcac gcgccttatt cttagcgggt cattttttac gtgccagcat cgtggcgaaa
     2281 cgcagtttaa tacgattacc gttggcgtcg gtgcggtgca gctcgccgac gtcttcattg
     2341 tatttcaccc tctcccagcc ttcgtaatat cgacgtaatt ctccctcttt gaaggcaaac
     2401 gggaagccga cggtacatgg ataatcagcg gtatccatcg ccgccacaat caggttgtaa
     2461 ccaccaggtt tagtgcaacg ttgcatattg gcaatcagcc ctgggatggt tttagcctca
     2521 aggaacatca gtaccacagt cgaaagaata aaatcgtact gtctatcaaa tgtgaggtta
     2581 ttcagatcaa cgactcgggt gtgtaaatta tccagatttt caatggattt aatgcgctct
     2641 acattggcga tactcatggc atttttatcc catgcgtcaa catcataacc attggctgcc
     2701 aggtaaagac tgttacgacc attgccacag cccagatcca gcgttttacc cggtttaacc
     2761 actttcaccg cttccagtac ttcagagtgt gtgcgggtta attcatattt atcagtaaaa
     2821 tagttttcgt cacgaatgat cattctttgt cctctgcttt cattaaaacg gcgcgctcgg
     2881 ttctgactaa caattttccc tgcatcagaa gcgcaaaagt acggatgagc agtattgcaa
     2941 taataaaatt ggtaaagata aacagcggta ccgccagcgt atggaaaaat ccattttcgc
     3001 tgccactccc cagatgcaaa ccggtggttg ccagtgcaga tacgccgaac gagaaactcc
     3061 agaatgaagc attaaatggc tgggagagat accatggcat cagacgtagc ataaacagca
     3121 gttgcagcag tccataacca aaaagcattt tcgccagcgt atcaccctcg ccgccgttga
     3181 cgctcaacca ggcactacag gccaccagcg caggagcgag ctgaatgccg agtgatgtcc
     3241 gcagtgccgt gggtaattct cccgaactgc gcagacgctg caagatcaca ggttcaaggc
     3301 ttagccatga gaaaacgcct gcgcctaaaa acaccagacc ggcgtcggtg tagcccaacg
     3361 caccacaggc cattgcgctg ataaagttgt tggcaactgt cggcagatac agtccaggcg
     3421 tggtagcttc ttcagggtga gatccgcgcc ataatcccgc agtttgccag gcggcataag
     3481 ccaactgaac cacgacacca aaactgaaca ggcacaccgc cagtgggcga aaccacggaa
     3541 caaaaccaat cgccaccagc atcgtcgttg ccggaaacaa actcacaaaa ctgctcagca
     3601 ctggatggcg aacttccgcc agcacgctat gcggaaagcg tatgagtcgg gtaataaatg
     3661 cgctagttaa taatccccag atgatcatcg ccagaatcac cagcccatcc cctaaccagt
     3721 ggctgacctg ccaaacctgg ctggcatagc gccaggcaaa tcccatccct atcgtcccca
     3781 acacaatacc aaagtagcct gccggcaaat tgagcacttt atcgctctgc atcttcttac
     3841 tcattttgtt taatttataa agtatatttg aaatgcattt tatggaattt tctcgagcat
     3901 agccagagcc gcagaatttg ctacggttat agtaagtcac ctgtgggaaa acgctatgcg
     3961 caaatatcgt ctaagtgaag aacagcgagc ctttagttat caggaagatg gcactaaaaa
     4021 aaatgtgtta ttacggcaga ttatcgccat tagcgatttt aacgatgtga tagcaggaac
     4081 cgctggcggc tggatcgatc gcgaaacggt gttagcacag gaaggaaatt gctggattta
     4141 cgaccagaat gctattgcgt tcggcggaac ggtgatttcc ggtaatacgc gcatcaccgg
     4201 caccagcgta ctgtggggag aggtttacgc aacggataat gtctggatcg ataatagtga
     4261 aatcagccag ggtgcatata taagtgacgg tgtcaccatt catgactcac tggtttgcgg
     4321 gcaatgtaga atatttggtc acgccttaat taatcaacat tctatgatcg tcgcagcaca
     4381 aggcttaacg cctgaccatc aactcctgct acaaatttat gatcgcgcaa gggttagcgc
     4441 ctcgcgtatt gtccaccagg cacaaattta tggcgatgcc gtcatccggt atgcttttat
     4501 tgaacatcgt gccgaagttt ttgattttgc cagtattgaa ggtaatgaag agaacaacgt
     4561 ctggctatgc gattgcgcta aagtctacgg tcacgcgcag gtgaaagctg gcatagaaga
     4621 agatgccatt ccaacgattc attacagttc gcaggtggcg gaatatgcca ttgtggaagg
     4681 taattgtgtg ttaaaacatc atgtcttgat tggcggaaat gccgtggtac gtggtggacc
     4741 gattctgctc gacgagcacg ttgtaataca aggtgagagc cgtattacag gtgcggtgat
     4801 aattgaaaac catgtggagt taaccgacca cgccgtcgtt gaagcctttg atggcgacac
     4861 ggttcatgtc cgtggtccaa aagtcattaa cggcgaggag cgcattacgc gaacgccgct
     4921 ggcagggtta ttatgaatcg ataatacgcg cgtataagtt cacatcatca taggcatcat
     4981 tcaggaactc agcctgtttc aggcaacctt caaggataaa accattgcgc aaagcgacct
     5041 ggttgctttg cggattgtcc acccgacatt tgatcacgaa gcgtctaagt tcaccagact
     5101 gggcgtaatg atgaatcaat gcctgcagcg cctgagaaat gatcccctgc ccctgatgag
     5161 attcgtccag ccagtagcct atttcagcgg ttttattcag tggttcaata cgattaaacg
     5221 agataacgcc gataagttca tcttctttga aaatcatgaa cattttggca tagccgcgtt
     5281 gatgcaacat cacattaccc tgcaccgttt ttcgcgtgtc ctcttcactc tgaacgaact
     5341 gcggccagtt tagcgactgc tgtaaccagg ttttattttt acagattaac tgataaagag
     5401 gagtgacgtg attttctgca acagcatgta attcaagtga ttcgcttact tttatcgttt
     5461 cagtcatctt atctccagtc aatccatgca gttgttaaaa aagccgcctg gctggcggct
     5521 taggaaagtt tacacctctt tgacgctctc ctgctgcagg attttgagca tctcatcttt
     5581 taactgtagc ttttgttttt tcatgcggac cacttccgca ttgtaccctc gaccgtcgga
     5641 accttccttt ctggcaattt catgatcaag tttattgtgt ttatcgaaca aggacataaa
     5701 gcgaggattt tcgtttttca gacgggatat taaatctcga tattctggaa acatacgcac
     5761 tccctgttag tggctgacaa ggtgtatctg aaatacacac catcagatta ctcatttaac
     5821 ttagggaaaa atgcgagcgg gatcgtgttt aaccagacgg cacctcccga agaaggtgcc
     5881 gaagaaaaaa cgattaactc atcacgcggt cgtcaacata ctgacgttta tccggcgctg
     5941 gcgggaaata ctgatacagc catgtttcac tgatagcgtc gccctgacaa cgcagataca
     6001 tccgcatatc gaccggatca gtggagtccg aagtcggata ccagtcaaac tgaatacgat
     6061 aaccatcaat gggttcaata tagagaattt cgatttgctt cgcttcccca ctggaaagcg
     6121 taatcaccgg ctcaatgcct tttggtgcgg cagctttcag atcaccacca acgaaatcga
     6181 cggcaaaacg acgcgcccat ttttcgggat agtgttcacc tggtgcccaa ccttccggga
     6241 aaccgcccat gccggtacgc gtcgccataa cgcgcgctaa tgggcaatga acaggcggtt
     6301 gcgcactcca gtacagacga tactggaatg caaactcatc acccgccttt acagcttttt
     6361 ctggctgcca gaagcagaca atgttatcca gcgtttcgcc cgttgttggg atttccatca
     6421 ggccgatggt gcccttaccc cacttgttac gcggttccac ccacagactt gggcgtttgt
     6481 tataccagcc cataatgtcc tgataatggg agaaatcacg atccagttgc aataaaccaa
     6541 accctttcgg gttgttgtcg gtgtaagcat tgaactgcaa tttttgcgga ttattcagcg
     6601 gacggcaaat ccactcgccg ttgccccgcc acatggacag acgatcagag tcatgaattt
     6661 gcggatgaat ggtgtcgcac atccgacgtt cattagtacc gcagctgaac atactggtca
     6721 tcggcgcaat gcccagctgt ttaatgtctt tgcgcgcata caggtgattt tccacatcca
     6781 taatcacctg acttttctca caatggatag tgaacttata ggcaccagta atgctggcgc
     6841 tatcgagcaa cgcataaacg gtaaaggtag ttgcccccgg ttttaccgta tcaaaccaga
     6901 aggcggtaaa gtcggggaac tcttctttac tgtcggtgta agtgtcaatc gccaggccgc
     6961 gggccgacaa accgtattga tatgtgtcat caacggcgcg gaaataactc gctccgagga
     7021 atgatactac atcacggcgc gccagttcgg gggctttaaa cacgcgaaaa ccggcaaagc
     7081 cgagatcgct ttgcccttct aattgtttgg tatcaacacc tgcatcgttg tatttgaaca
     7141 actccgggcg aaagtgaatt tcacgcgcca gatgtgttgc tggatctaca gaaaacatac
     7201 gaacgcggcg acggaatccc attcccatat ggaagaactg agcgtccagt tgacggttct
     7261 caacgttatg ccagagcgat ttttcggcgt cgtattgaat actgttataa gcctgcggcg
     7321 tcattgtcgc cagcgtgtca ggtaacggac gaggcgcacc acgccacgct gtttgcgcta
     7381 agtcgtgcgc cattgactgt agaatggaga agtcaaaacg ctgggtttgc ccgtcggcaa
     7441 tatcagaatc tgccgcgaat gccgcctgag aaaaaagaga agcaatgccg ctggtaccgc
     7501 acacggcggc catagccatt gaacctttaa taaatcgtct acgatccata cctgaaagtg
     7561 agtccttctg gtgttgtgaa tggtcccgcg ctggtcgttt tcagcggcaa gaaagtgctt
     7621 atttatgacc gctcactcta gacaatattt attaataatc caattgttga actcagagaa
     7681 tatgcataaa tcggcaattt ttttatgtcg atgggatgag cctgattcgt ttgtaaaatc
     7741 aggcaaattt ttgcaaaggc ggatggtcag aatttaaagc aaaccacagc gacgtttact
     7801 gcgttcttcc gcttcctgat aaagctggaa ctcatccaaa accggacaac ccaacgcctg
     7861 ctcaaatgcc tcgcgactgg cattaccgtg attgcgtgcc tgcgtttcat tacccagatt
     7921 tgactggaag atccccgccg cgctaacggg caagaaatct tcataggtaa tgggttgcgc
     7981 cgcgacccaa ccacgttcaa ttaagggctg tggatcgtct ccgggatgaa tcgcctgacg
     8041 atgcgcctca cctgaaggcg tcagacggta ccggaaccat gccagccctt gctgacgcat
     8101 taaaaactca ctgtcaggaa aagtgcggaa ggtttcctgt aaatgcattt ggtgagtgag
     8161 attatcctgc ccggttccag cgttacgcag aagatcatca tacagttgtc gccctttcgg
     8221 cgttaatgcc acgccacgct gctcaatttc accaaagcgc gcggtatgcg tgccctgttt
     8281 ctgccccgca aacaacaccg tctcttccag tgctttaaag ctggtctggc gtagtaaaat
     8341 cggtacctcg cggcgcggcg gcccctcgat gagaattttg ggttcaattc cgcattcagg
     8401 catcatcgac tgcacccggt caatatccag cgtacgtggc gtcaggtggt tgatatggca
     8461 tccaggaaaa cagaccacat cagcaatcaa ccgatgttcg ttgtgcaagg cgcgataggt
     8521 ttcttcatct accgttgctg actggtgcca gcgaaacgtt tccagggctt cctgcacaaa
     8581 ctcctgtgcc tgtgtttcgt taaaaccgcc ctgctgctca tattcctcta acagttgtcg
     8641 acaacgtggg gtgaagatat cgcgctgacg cagaatctcc gccgctttct ggcgcaaaat
     8701 ttcgttctcg ataagctcaa ggcggagtaa ggaggtaaaa acgcggaagg gattacgcgc
     8761 cagagaagca tcatcaatgg gccgaaatgc tgtcgaatgt accggcaccc ctgcctgcga
     8821 gagatcgtaa tagctcaccg ggtacatccc cataatggca aacatccgcc gaagagtagc
     8881 aagctcttgt gcagtcccaa cgcgaatcgc cccatgacgt tcaacattca gtcgcgccag
     8941 ctcgtctgca tttaccattt tttcgtgcag ttgaggattg ttttccagca cagccagatt
     9001 cacatcagct accagttcca gcagcgtgcc atattgcgga acttcttgct ggtacatggc
     9061 tgacattgcc tgcgaaaact gttcccgaat ctcatccgcc gtgatgctgt tcgccatagt
     9121 gtctgcctcc agtggtcagt aatctggagt gtaggtaacc gcattcactc tggcgggaag
     9181 aatttacaaa ctgtgatctc gccgcgaaaa catcaatatt atccattttg ctgtaacaca
     9241 attgctttaa ttgttaataa tattttgcaa tcaagttatc ataatcaaac aacttcactt
     9301 gtcagcgaca ccgcttcgtt tttaacatcg cttatggaaa aaaatagtct gtttagtcag
     9361 cgcatccgtt tgcgccacct tcatacattc gtagctgtcg cacaacaagg aactttgggg
     9421 cgcgcggctg aaacccttaa tttgagtcaa cctgcgctct ctaagacatt gaatgaactg
     9481 gagcagctga ctggcgctcg cttgtttgag cgtggtcgtc agggggcgca acttacctta
     9541 cccggcgaac aatttttaac gcatgcagtc agagttcttg acgccatcaa cactgccgga
     9601 cagtcgcttc atcgtaaaga aggtcttaat aatgatgtcg tcagggttgg tgcactacct
     9661 actgcggcac tggggatatt accttcggtt ataggtcagt ttcatcagca acaaaaagag
     9721 acgaccttgc aagttgcgac aatgagtaac cctatgattc tggcgggttt aaaaaccggg
     9781 gaaatcgata tcggcattgg tcggatgtca gatcctgaac tgatgaccgg gcttaattac
     9841 gaactgctgt ttcttgaatc gttgaagctg gttgtccgcc ctaatcaccc gctacttcag
     9901 gagaacgtaa cgctaagccg ggtgctggaa tggccggtcg ttgtatcacc agaaggcact
     9961 gcgccacgcc agcattcaga tgcattagtg cagagccagg gctgtaaaat tccttcgggt
    10021 tgtatcgaaa cgctgtctgc ttcgctatct cgtcaactta cggttgaata cgattacgtg
    10081 tggtttgtcc cttctggcgc tgtaaaagac gacctgcgtc atgccacgct ggtggccctg
    10141 cctgttccgg gacatggtgc aggcgaaccg attggaatac tgacccgcgt agatgcgacg
    10201 ttctcttctg gttgccagtt gatgattaac gctattcgaa aatcaatgcc gttctgaaag
    10261 gtgaagggat ctgtcgatcc ctccttgaac attttcacac cgtagcgaaa ctaactggtt
    10321 cacccgctcc gcgaggttct gccgacacag aatgtttgtg cagacggaat acatccaccg
    10381 cctcagttaa tcgtgccgcc tgttcttcaa gggacaccgc cgccgctgag gcctcttcta
    10441 ccagagaagc attctgttgc gtcaccttat ccatttcaga aatcgcctgg ctaacctgcg
    10501 ttatgcctct actttgttca tccgaggcgg cggcgatttc ctgcatgata tgtgtgacac
    10561 tcgcgacggc atcaacaata gtgctcatgg ttttccctgc cgttgccacc tcatccgacc
    10621 ccaggtcaat taacctgact gattcactga tcaagccttc aatctctttc gccgcttggg
    10681 cgctgcggct tgcgagtgtc cgtacttcgc tggcgacaac ggcaaatcca cggccttgct
    10741 cacccgctcg cgcggcttca acggcagcat tcagtgccag aatattcgtc tggaaagcaa
    10801 tactgttgat gacggcggtg atctcggaaa ttttcttcga acttgtagag atagcgccca
    10861 tcgtttttac tacaccggaa accgtctgcc cgccatcgct ggctttaata gaagcctctt
    10921 gcgccagttt gctggcatga tgcgcgttat ccgcattctg tttcaccgtc gcagtgagtt
    10981 gctccatgct agcggcagtt tgttcgatag ccgccgcttg ttcttcggtg cgagatgaca
    11041 ggtccgcatt gccagctgaa atttcgctgg tgccacgata aatctcttcc gcaccctgtc
    11101 gaacagtccc tactgtcatc cccagtgaat gctgcatttg ctgtaaatga cgacttaagc
    11161 gaccgatttc attacgaccc gccggttcat cattcatcgt cagatcgcca ctggcgattt
    11221 tttcaatccg ttgtgcggca tgttgcagtg gacgaatgac gatccgacgt agcaccataa
    11281 atgttatcag cgtcatgacc agggcaagca caaacgcgcc aatcatgaac atcccaccca
    11341 ggcgggtgcg ctgatgggcc agttccgcca gttggttggc tctggtgcta cgtatcttaa
    11401 cggctttgtt caaaatatcg gtataagcat tatccagcgg tcggatctgc tcactttcat
    11461 gattgataat cgcttcaaac atgccatttt tggcatattt caacataggt tgcatacccg
    11521 tgatataagc ctgaaagcgt tgatttaatt cagtgtcgag ggcttcatca gcaggtgttt
    11581 tcaccggtcg attctgataa gcacgataac cttgctgcga ctgtttaatc tccgattcgg
    11641 cttgcgcaat atttcgcttc attgcttcca tttccgcaat acgacttgca gccccggcct
    11701 gaatcatatt gatccgggct gaacgcaaaa aatctgaact gttcgctaac ccggtccgaa
    11761 tctcaatctc cgcagtaaca tcacgctgat cgcgatcggc ctgccagagg aaatagccag
    11821 ccagagcaga acttaatgcg aatagaacta agataccgcc aagaatgcag gcaaataacg
    11881 gaaccagcct gatgtgatgc aaaaaaccta atcgatgtga gggagttgta ttcattaccg
    11941 ttgtctctcg tccaggttta ctcctgatag aaagtcatcg gcacttgacg gtaattactt
    12001 atgaccaaaa acctctttgc gtcacatttt tacaacaatt attgcagaga aaattaatgc
    12061 tattggaatg aagagagtaa cgaatggcta aactggctaa cccgaatgac gaaaaattct
    12121 ggaaaacagc acgcaatttc gtgaagtgat tattttcgtc cctaaggaaa cctcatagga
    12181 tgcctgcggg tgcctgagac tttctgtttt caggctaacc gggaatcaga aagacgaagg
    12241 ccccgaccga tatttctatc aaccgaggcc aacgaatgaa cctagcaatt cagatcttag
    12301 cctcttaccc gccatccggc aaggagaaag gctatgaagc acaaccctct ggtggtgtgt
    12361 ctgctcatta tctgcattac gattctgaca ttcacactcc tgacccgaca aacgctctac
    12421 gaactgcggt tccgggacgg tgataaggag gttgctgcgc tcatggcctg cacgtccagg
    12481 ttagggcaag cgcggggatt ttccccgcgc atctgactag cagattcatt ttctcatggc
    12541 acccctttta cacttcagtt agtgcatgtt tttttcgagg cactaataga accatcatta
    12601 cagacaaatt tgccgcctgc cgtacagtgt gagatccccc ctttcgaacc agaacagggt
    12661 ttgcgcccgg catcggcata aaaggaaaca agcgttccca taaacaaaat aagtgccagt
    12721 tttttcatat aacctccatg gtttatcgaa tcttatgttc gatttgaacg ttatacgctg
    12781 gtttattttg ataccgttga tgagatcaaa gatcgggtga attgattaaa aataagcca
//
LOCUS       DAZFRJ010000130        12656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000130 DAZFRJ010000000
VERSION     DAZFRJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.131"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..1570
                     /gene="caiT"
                     /locus_tag="ACHKUN_003319"
     CDS             56..1570
                     /gene="caiT"
                     /locus_tag="ACHKUN_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO6661528.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1601..2743
                     /gene="caiA"
                     /locus_tag="ACHKUN_003320"
     CDS             1601..2743
                     /gene="caiA"
                     /locus_tag="ACHKUN_003320"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO6661529.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2872..4089
                     /gene="caiB"
                     /locus_tag="ACHKUN_003321"
     CDS             2872..4089
                     /gene="caiB"
                     /locus_tag="ACHKUN_003321"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO6661530.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4163..5716
                     /gene="caiC"
                     /locus_tag="ACHKUN_003322"
     CDS             4163..5716
                     /gene="caiC"
                     /locus_tag="ACHKUN_003322"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO6661531.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5825..6610
                     /gene="caiD"
                     /locus_tag="ACHKUN_003323"
     CDS             5825..6610
                     /gene="caiD"
                     /locus_tag="ACHKUN_003323"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO6661532.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6616..7206
                     /gene="caiE"
                     /locus_tag="ACHKUN_003324"
     CDS             6616..7206
                     /gene="caiE"
                     /locus_tag="ACHKUN_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO6661533.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7292..7687)
                     /gene="caiF"
                     /locus_tag="ACHKUN_003325"
     CDS             complement(7292..7687)
                     /gene="caiF"
                     /locus_tag="ACHKUN_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO6661534.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7948..11169)
                     /gene="carB"
                     /locus_tag="ACHKUN_003326"
     CDS             complement(7948..11169)
                     /gene="carB"
                     /locus_tag="ACHKUN_003326"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO6661535.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11187..12335)
                     /gene="carA"
                     /locus_tag="ACHKUN_003327"
     CDS             complement(11187..12335)
                     /gene="carA"
                     /locus_tag="ACHKUN_003327"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO6661536.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2958 a         3293 c         3364 g         3041 t
ORIGIN      
        1 aataaaacaa acgggaacgc aaaataaata ttcgttttca cagtggaatt aactcatgaa
       61 gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc tttccgccgt taataatcgt
      121 cggcatactt tgttggctta cagtcagaga tctggatgca gcgaatgtcg ttattaatgc
      181 tgtattcagt tacgtcacca atgtatgggg atgggcattt gaatggtata tggtggtgat
      241 gcttttcggt tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga
      301 accacctgaa tttagtaccg ccagttggat ctttatgatg ttcgcctcct gtacgtctgc
      361 tgccgtactg ttctggggat cgattgagat ctactactac atctccaccc cgccgtttgg
      421 cttagaaccg aactcgacag gggcgaaaga gttggggctg gcttacagct tgttccactg
      481 gggacctctg ccgtgggcca cttacagctt cctttcagtc gccttcgctt acttcttctt
      541 tgtccgcaaa atggaagtga ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa
      601 acacgccaaa gggttgttcg gcactatcgt cgacaacttc tatctcgtcg ccttgatctt
      661 cgcgatgggt accagtctgg gccttgccac gccgctggtg accgagtgta tgcaatggtt
      721 gtttggcatt ccgcataccc tgcaactgga cgctatcatc attacctgct ggattatcct
      781 caacgccatt tgcgtcgctt gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg
      841 tagttacctg agcttcctga tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat
      901 catgaactac ttcaccgatt cggtggggat gttgctgatg tatctgccgc gcatgttgtt
      961 ctataccgat cccatcgcta aaggcggctt cccgcagggc tggaccgtgt tctactgggc
     1021 atggtgggtg atttatgcta tccagatgag tatcttcctc gcccgcatct cccgtggtcg
     1081 tactgtgcgt gaactgtgct tcggcatggt gctggggctg acagcgtcaa cctggatcct
     1141 gtggactgta ctcggtagta acactctgct gttgatagat aaaaacatca tcaacattcc
     1201 aaatctgatc gaacagtacg gtgtggcgcg cgccatcatt gaaacctggg ccgctctgcc
     1261 actcagcacc gccaccatgt ggggcttctt catcctctgc tttattgcca ccgttacgct
     1321 ggttaacgcc tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga
     1381 agaaccacct ctgctggtgc gtatcggttg gtcaattctg gttggcatta tcggtattgt
     1441 tctgctggcg ctcggcggcc tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc
     1501 gctgttcttc gtcaacatta tggtgacgct ctcctttatt aaagacgcga aacagaactg
     1561 gaaagattaa ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga
     1621 tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc
     1681 ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc
     1741 ggatatgggt atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt
     1801 tgttactctc gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtgct
     1861 gtaccagttg ccgggcgggt tcaacacctt cctgcgcgaa ggcacacaag agcagatcga
     1921 caagattatg gctttccgcg gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc
     1981 gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat
     2041 ttatcttaat ggtagtaagt gttttattac cagcagcgcc tacaccccgt acatcgtggt
     2101 gatggcgcgc gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat
     2161 gagcaaaccg ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg
     2221 ctgtgaaatc acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg
     2281 taacggcttt aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg tagccctcac
     2341 caactacggt acggcgatgt gcgcctttga agatgcggcg cgctacgcca atcagcgcgt
     2401 gcagtttggc gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc
     2461 gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg
     2521 caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga
     2581 agttgtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat
     2641 cagccgcttc tggcgtgatc tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca
     2701 gatcctgacg ctgggtcgcg cggtgctgaa gcaataccgc taagtccgtg cgctgcctga
     2761 tgcgacgcta atgcgtctta tcaggcctac gggagcacga atgtaggccg gataaagcgt
     2821 ttacgccgca tccggcagtc atgcgctaca taaatttttc aggagctaat aatggatcat
     2881 ctacccatgc cgaaattcgg gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa
     2941 atcgccgggc cgttcgccgg gcaaatgttc gcagaatggg gtgcggaagt tatctggatc
     3001 gagaacgtcg cctgggccga caccattcgc gttcaaccga actacccgca gctctcccgc
     3061 cgcaatttgc acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga agcgtttctg
     3121 aaattaatgg aaaccaccga tatcttcatc gaagccagta aaggtccggc ctttgcccgt
     3181 cgtggcatta ccgatgaagt actgtggcag cacaacccga aactggttat cgctcacctg
     3241 tccggttttg gtcagtacgg caccgaggag tacaccaatc ttccggccta taacaccatc
     3301 gcccaggcct tcagtggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc
     3361 ttcccgtata ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca
     3421 ctgcataaag tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg
     3481 atgctgcgta tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg
     3541 cgcatgagca aaggtaaaga tccctactac gccggttgcg gtctgtataa atgcgccgac
     3601 ggctacatcg tgatggaact ggtgggcatt acccaaattg aagagtgctt taaagatatt
     3661 ggcctcgcac atctgctcgg tacgccggaa atcccggaag gcactcagct tatccaccgt
     3721 atcgaatgcc cttacggccc actggtagaa gagaaactcg atgcctggct ggcggcacat
     3781 accatcgcag aagttaaaga acgctttgcc gaactgaata tcgcctgtgc caaagtactg
     3841 accgtaccgg aactggaaag caatccacag tatgttgccc gtgaatcaat cactcagtgg
     3901 caaacgatgg atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac
     3961 cccggacaaa tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa
     4021 aatatcggct acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt
     4081 gaggactaaa cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg
     4141 aacaaaaatg gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg
     4201 ggacgatctg gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg
     4261 agtcgttaac cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct
     4321 gttttatacg ctggggattc gcaaaggcga caaggttgca ctacatctcg acaactgccc
     4381 ggaatttatc ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa
     4441 cgcccgcctg ttacgcgagg aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct
     4501 ggtgaccagt gcgcaattct atcctatgta tcaacagatt cagcaggaag atgccactca
     4561 attgcggcac atttgcctga cagatgtggc acttcccgct gatgatggcg tgagttcgtt
     4621 tactcaactg aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tattgactga
     4681 cgatacggcg gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat
     4741 tacccattac aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga
     4801 cgatgacgtc tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc
     4861 gatggcggcg ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc
     4921 cttctgggga caggtgcaga agtaccgcgc caccattacc gaatgtattc cgatgatgat
     4981 ccgtacgttg atggtgcagc cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt
     5041 gatgttttat ctcaacttgt cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt
     5101 tcgcttgctg acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgccc
     5161 tggcgataaa cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg aagcggagat
     5221 ccgcgacgat cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct gtattaaagg
     5281 cgtaccaggg aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt
     5341 gctggaagcc gacggctggc tacataccgg cgataccgga tactgcgacg aagagggctt
     5401 tttttatttc gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg
     5461 cgtggagctg gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg
     5521 tattaaagat tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga
     5581 aacattgagc gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt
     5641 gccctcttat ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag
     5701 aaagaatctg aaataaatga aacagcaggg aactaccctg ccagctaata atcacgctat
     5761 aaaacaatat gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa
     5821 aggaatgagt gaatcattac atctgacccg caatggatca attctggaaa ttacccttga
     5881 tcgtccaaaa gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct
     5941 aaatttccgt gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaagtt
     6001 cttttccgcg ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt
     6061 tggcccgggt ggatttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc
     6121 agctgtgaac ggctatgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat
     6181 tgtttgtgcc gataacgcca gcttcgccct gccggaagcc aagctgggca tcgttcctga
     6241 cagcggcggc gtgctgcgtc taccgaagat cctgccgcct gccatcgtca atgaaatggt
     6301 gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt
     6361 ggttagccag gcggaactga tggataacgc ccgcgaactg gcgcagcagc tggttaacag
     6421 cgccccgctg gcgattgcgg cgctgaaaga gatcttccgc accaccagcg aaatgccggt
     6481 agaagaagcg tatcgctata ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca
     6541 ttcggaggat gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa
     6601 aggacgttaa caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg
     6661 acggcgtttg tccatcccag tgccgtcttg attggcgatg tgattgtggg agccggtgtc
     6721 tacatcggcc cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga
     6781 gccaatattc aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg
     6841 gaaaacggcc atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca
     6901 ttggtcggga tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt
     6961 gccgccatga gctttgtcaa agcaggcttt cacggcgaga aacgccagtt gttgatgggt
     7021 acgccagccc gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat
     7081 accaaagagt atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacacagcca
     7141 ctgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa
     7201 cggtgatctt gccggatgcg acctggttgc tggataagat gctacaacat cgcatctggc
     7261 aagcaggacg cccggtatca acgagataaa attaacgacg catactcttt gacaacatca
     7321 tctgccactt ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata cgcggcgtct
     7381 ttcttacgcc gactaattta tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat
     7441 cgatatcgac cacttttacc agtcgctggc actggcatcc ccgtccttcc agcttattag
     7501 ggatcatctt aacttcgcag cttatttctg cgacttccga cagaatataa gtcagggtat
     7561 taaccgcctt gctgtgttca atatcgaaat gaatagagat ctctcttgct atcacccacc
     7621 gattttcagc catcatccat tcggcgatta acaagtagag tggtttttca acatatcctt
     7681 cacacatacg aactccagtt tcacaaatta aaagtctggc tatgactcgt tttgaagcaa
     7741 ttaaacaagg ccatattaaa attgtggata tatcgatagt atgcaacttc accgtcgata
     7801 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
     7861 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
     7921 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
     7981 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
     8041 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
     8101 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
     8161 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
     8221 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
     8281 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
     8341 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
     8401 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
     8461 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
     8521 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
     8581 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
     8641 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
     8701 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
     8761 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     8821 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     8881 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     8941 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     9001 ccgaccgccg agaacgtaag acggacgtac caccagcggg tagccaatct ctttcgcctt
     9061 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     9121 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     9181 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     9241 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcta gcacatcttc
     9301 cagtgttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     9361 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagtgcca gcgaggcgtg
     9421 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     9481 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     9541 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     9601 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     9661 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     9721 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     9781 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     9841 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     9901 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     9961 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
    10021 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
    10081 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
    10141 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
    10201 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
    10261 gcgcgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
    10321 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
    10381 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
    10441 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
    10501 atcacgcacc acttccatct cgtactcttt ccagccaatc agcgactcat caatcagcaa
    10561 ctctttggtc ggagagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
    10621 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
    10681 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
    10741 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
    10801 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
    10861 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
    10921 cgggcgctct ttttcaataa tcttgcgtac cacttcccag tgaatcggct cgatgtaggt
    10981 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
    11041 ccggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
    11101 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
    11161 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
    11221 tcgataaagt ggtcgaacaa cggcgcggcg tcgtgtggac cagggctggc ttcagggtgc
    11281 ccctggaagc tgaacgccgg tttatcggtg cgatgaatgc cctgtaacgt accgtcgaac
    11341 agggatttat gcgtgacacg caggtttgct ggtaatgttg cttcgtccac cgcaaaaccg
    11401 tggttctggg cggtgatcat caccacgttt ttctcaacat ctttaaccgg atggttgccg
    11461 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
    11521 tgaccgaggc agatgccgaa gaccggaata tcggtttcga ggaatttctg gatggcggta
    11581 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat accgtctgga
    11641 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
    11701 ctgtccacca acatgcgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
    11761 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
    11821 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
    11881 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
    11941 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
    12001 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
    12061 aagttgctgg caatcagcgg caggtcgcga atcaccagac cttgtgcatg tacctgagaa
    12121 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
    12181 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
    12241 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
    12301 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
    12361 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
    12421 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
    12481 ggatttttta tattattttg tggctcatgc atgttttaca ggcattaagc ggcaaaaaga
    12541 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaagcga
    12601 gtagggaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa ccgaag
//
LOCUS       DAZFRJ010000131        12535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000131 DAZFRJ010000000
VERSION     DAZFRJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.132"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..347)
                     /locus_tag="ACHKUN_003328"
     CDS             complement(<1..347)
                     /locus_tag="ACHKUN_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6661537.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGER"
     gene            complement(344..778)
                     /gene="psiB"
                     /locus_tag="ACHKUN_003329"
     CDS             complement(344..778)
                     /gene="psiB"
                     /locus_tag="ACHKUN_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6661538.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(833..2791)
                     /locus_tag="ACHKUN_003330"
     CDS             complement(833..2791)
                     /locus_tag="ACHKUN_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6661539.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            complement(2850..3083)
                     /locus_tag="ACHKUN_003331"
     CDS             complement(2850..3083)
                     /locus_tag="ACHKUN_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO6661540.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            complement(3141..3668)
                     /locus_tag="ACHKUN_003332"
     CDS             complement(3141..3668)
                     /locus_tag="ACHKUN_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6661541.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            4038..4106
                     /locus_tag="ACHKUN_003333"
                     /pseudo
     CDS             4038..4106
                     /locus_tag="ACHKUN_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(4440..4631)
                     /locus_tag="ACHKUN_003334"
     CDS             complement(4440..4631)
                     /locus_tag="ACHKUN_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661542.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(4628..5050)
                     /locus_tag="ACHKUN_003335"
     CDS             complement(4628..5050)
                     /locus_tag="ACHKUN_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6661543.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(5097..5522)
                     /locus_tag="ACHKUN_003336"
     CDS             complement(5097..5522)
                     /locus_tag="ACHKUN_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6661544.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGNDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(5938..6708)
                     /locus_tag="ACHKUN_003337"
     CDS             complement(5938..6708)
                     /locus_tag="ACHKUN_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661545.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(6753..7220)
                     /locus_tag="ACHKUN_003338"
     CDS             complement(6753..7220)
                     /locus_tag="ACHKUN_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6661546.1"
                     /translation="MRALKIEKETRMYGTCETLCRELAAQYTENTPLMLVVWSPEEIQ
                     ALADGMEISLTGHEIRTVLARLEDIPEEQRIESGISSAAVMEIINNVSENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(7201..7422)
                     /locus_tag="ACHKUN_003339"
     CDS             complement(7201..7422)
                     /locus_tag="ACHKUN_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661547.1"
                     /translation="MNYAGHEKLRAEVADVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            complement(7423..8106)
                     /locus_tag="ACHKUN_003340"
     CDS             complement(7423..8106)
                     /locus_tag="ACHKUN_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6661548.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAAFQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(8182..8418)
                     /locus_tag="ACHKUN_003341"
     CDS             complement(8182..8418)
                     /locus_tag="ACHKUN_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661549.1"
                     /translation="MRRLTRRGINAACGQCPASGRARDGRFYRSRLAPVTVYASPSPF
                     SDERPSSRFRGITLPSKRRRLRYSTVGLTRYRTR"
     gene            8310..8540
                     /locus_tag="ACHKUN_003342"
     CDS             8310..8540
                     /locus_tag="ACHKUN_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFO6661550.1"
                     /translation="MSPVPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLSEKKRAVARPSPSYFIRHNAPPCRLSTQVR"
     gene            complement(8491..9393)
                     /locus_tag="ACHKUN_003343"
     CDS             complement(8491..9393)
                     /locus_tag="ACHKUN_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6661551.1"
                     /translation="MYFSAEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(9502..9771)
                     /locus_tag="ACHKUN_003344"
     CDS             complement(9502..9771)
                     /locus_tag="ACHKUN_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661552.1"
                     /translation="MTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDGRFYRSRLA
                     SVTVYASPSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRTR"
     gene            9942..10904
                     /gene="parM"
                     /locus_tag="ACHKUN_003345"
                     /gene_synonym="stbA"
     CDS             9942..10904
                     /gene="parM"
                     /locus_tag="ACHKUN_003345"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO6661553.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHSQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            10907..11257
                     /locus_tag="ACHKUN_003346"
     CDS             10907..11257
                     /locus_tag="ACHKUN_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6661554.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTITHPSSQQEQR
                     SDEETKKNAMKLIN"
     gene            11395..12447
                     /locus_tag="ACHKUN_003347"
     CDS             11395..12447
                     /locus_tag="ACHKUN_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661555.1"
                     /translation="MKDTITPLNYTKEAIKDLSVMEKGLRSNAPLTSYIMRQVIDMLD
                     SSVKFILPNCCDIIEPEEYRQTHFDLARLPYPVVTFEIPWFKDSVETQIGDFNISPSS
                     RRIALCWEARQSFEPIPGCNSILNTYIDGGVFILPVSWSDDLKIWILGVGGMFFPYNN
                     KLTKYEPDRTLPASRLVIDTLKENGVAKYNAAHFKAEPFITSMEFKDDLIKQVGSIER
                     LYAQIIMDTRDELQAFIQACSVLNCENVCPVTLSTKPERKFINGRKVQPPEKNKRPSY
                     TYKVLQLSETKVQSNHTGTGKSGGTKRMHLRRGHIRRKNNKLIWIRPAMINANSRAGI
                     VDKDYQINIRKEENKP"
BASE COUNT         2992 a         3580 c         3211 g         2752 t
ORIGIN      
        1 cgctcacctt cacgaaaagg aacggacggg aacagacgat gccccgcatc ggatggcagg
       61 ggaagaacac agacttcacc aaaactttca atcagtttat ccacggcagc cagccacagg
      121 agacggttgc catgaaattc ctccttcgtc agcgcaggag caaaaaagcg gagatcggtc
      181 agcgaaatcc ggcgactgcc attcaggcag cgaaagaaag cgctggcata aggccatgct
      241 gaaagtgtgc gtccctgacg acgacgttgt tcggtgacag ccacagcctg catggcggcc
      301 tgttgctctg cgctgagggg gatcagtgcc cgtgaacgtg cgctcatgcc tgacctcctt
      361 ctgccatcag gatatggatg atatcgttaa cactgtaccc ctgcgcatca agtgtcgcgg
      421 caagcgccag cgtgtgactg atagcgacag gcgtgaagat atgttgcacc tgaacgacgg
      481 cgaaaggctg tccaccacaa ttcaccagaa ccagcatcca gagctgagag acgtctcccg
      541 gcgaacataa tgccaggtgg aaacgttcgt gggctggcgt gaaacgaacc tggacgggga
      601 aaaagccccc gaactcactg ccatactcgc cattcatcat ccagttagcc ggtgcgtcca
      661 cttcacgcat cacctcgtga gtgagattac ggcgcatatc gcttcccgca gcacggatat
      721 cttcatattc ctgtgcgttc atggactgca gggcattcag ggtcagttca gttttcatca
      781 cggtatctcc cttgctgccg ccccgtctcc ggcggggcgg tgtggttgtt catcaggcgg
      841 catcagccag gttatcggtg cgttcagtgg catctgtctg tggatgtggt gcacacatcc
      901 agccaggcac ccagcggttg tctttcatat ggtgctctgc atgttcagcc gcatcacctt
      961 tcttcatctt ctccgcgtcc cgtgcggcac cggacagtcc tgcctcattc agggctgcga
     1021 taatctgcgg ctttttcagg tgtccgaaga agtttgcttt tgtcggctgc caccagtcgc
     1081 gcatgtgaaa gccgatggcg ctttccagcg aatccagcgg actgcgtgac gtgtgaccac
     1141 actcacgggt ctggacaccg ttaaggctgc atgcggtgca gaaactgagc agggataaca
     1201 gcacctcctg actgagtgac aggaatgtcg tcatgtcccg tgaccatccc tccggtaaca
     1261 gggcagcaag acgggctttt tctgccatca gcgccatgaa tgcggcacct tccttccccg
     1321 atggcgcatc gctggtcagc gaataatgtt cacattccag gctgatttgt gctggtttac
     1381 tgtacgcccc gctgccaaac acattcaggc agagcgtcca tgccagcagt gccagagatt
     1441 tgtccggctg ctgcatgagt gccgcctgga ctgccagcgt gcgttcggaa gacattttcg
     1501 tcagcagtgg cagactgatt tcctccactg acgctttctc ctgcatctgt tccgtgcggt
     1561 cagcgtcgtc agccacgtca tcctcactgc gcaactgcac accacgctgg acacacacgt
     1621 tgccataacg ccaggacacc accacaccgc ttccggcacg catctccggc gtccacgctc
     1681 tgaccttcgc catgcagcgc atcagcttca tttctgcttc cagcaggtcg gattcctcac
     1741 actgattttc cagcgcgtcg taacgcgtca tcagttcgtt caggcgttct tcttccgcct
     1801 ccgtcagcac cgcttccggc tccggcagac tgcggtatgt tccggcatcc tcacggcact
     1861 caccgacagg ctccatgcgt ccggcgcacc attcccagcc ttcggcttcc cgaaggtgtt
     1921 cagcgacagc ctggagtttt tccagcaggg cagcatcgag cgcaacacag tccacatagc
     1981 caccctcgtc gtcgctgaac aaatcggtgc gcagttcatc tggcgagaag gcatcagccc
     2041 ccacaaagcg gaatttagtg ttccccgcca ccgccacttc actttcggtc accagacgac
     2101 gaatggtctg tacatccggt tttccgcccc atcccgactg gcaggcggct tcaaacacct
     2161 gcacctgacg cgcggtgtcg ttctccagcg ccagcgcctg acagtgctct gtggtgatgc
     2221 ggtcttctgc cagcgcatcg aggatgacag gcgcaaggtc tgccagtttc agcattcgct
     2281 gaacgtggcg gggcgaatag cccagcaaat caccgatttg tgcaggcgtt ttgccttcct
     2341 gcgccatcgc acggaatccg gcaatctgtt cggcagggtg catatcccga cgatgaccgt
     2401 tttcagtcat tgatgcggca gtcgccagct cctgcggaat aaccttcacg cgtacaggcc
     2461 agtcagccgg aatgatgcca cgctctgcca gcatgttgag tgcagccagt cggcgaccac
     2521 ctgcggcgac accgtaacgg tcaccaggca gggtatgcac gaccagattc tgcagcagtc
     2581 cgacgccctt aatggactcc gccagttcgc tgacggactc cgcagaatac ggcaccgtgc
     2641 gcacattcag cggtgattta atcagcgagg ccagcggcac gctcacttcc tccgtctgcg
     2701 ccagcagggc agacaggaca gtttcctgcg tttttacaga tttacggctt gctttgcggg
     2761 tggtttctgg ttcacacttc gttacaggca taatcatccc cttataattg cttatgtcct
     2821 gccggacgtt cgcgcgtccg gcacctctct tacttaagaa tcccgaaatc cctgatgtac
     2881 tggttcatcc agtacccggc accgtcctca aaattccagg agcgccagat caacgtgcca
     2941 tcctgacgga caaccaggcg aaaatgcgtt ccctgatcat cctcgataaa gacgttccgg
     3001 tactgtgcgg caacggcttc cgcctgttcg cgggtaaagg agccgtccgg cagtccctga
     3061 agtattctga aatattcgct catcctcata tctccggtga tgccccgcac agggcggggc
     3121 ggttgtcaca gtcagcccga tcagaacggg atgtcgtctg aaaacccgta atcctccccc
     3181 tccggcggtt gcggctgagg ctctggctgc gcggccttac gtccacggcc tttcgttttc
     3241 gcgccacctt ttttcgtcgc gtcagcactc tgtggctgcc cattctgctg aggcttcggt
     3301 tgcgcctgag cgttctgctg tggtgcacgt cccagcatct gcacggtgcc cgtggtctta
     3361 acaagaattt cagtgacgta acgggtgata ccgttatctt cccagctacg ggtacggagt
     3421 tgcccctcga tgtagacctg cgcgcccttg cgcagatatt caccagccac ttccgcgagc
     3481 ttgccgaaca gcaccacgcg gtgccattcc gtctgctccc gcatctcccc cgtctgtttg
     3541 tcgcgccagc tttctgacgt ggccacctgc aggtttgcca ctgcgccccc gttggggatg
     3601 taacggactt ccggatcatt gcccagacgc ccgacgagga tgaccttgtt gataccacgt
     3661 gcactcataa gaacctcctg actgaaagat gtgagataaa ccggataccg gcgacgggac
     3721 gttcatgtgc cccttcagag gagcgaaagc tcctcctgcc gtccacttca caggggtttc
     3781 tccccgcagt ggacctttcc gatgggggcc cgtttaccgc ctcacggggc agctgtccgg
     3841 gggcggacgc aaaatttttt gccgggttca tggctaaaaa ttttgtggcc ggtgcatctc
     3901 accctggaca gatggaacgg gagggggtta cgtctgcccc ccggaaagtc cacagcgggg
     3961 aaacaggccg caggggggac ggcaggaggg aggactgctg cgccatcagg cgcagtttcc
     4021 tcacacaccg tcaggtgtgc agggagcgca ggtcacggcg cgacaggggc ggcgcgaccg
     4081 gaacggtcgc tgcaagcccg ggttttcacg catcggaccg tagcggcagc ccgaaaccgc
     4141 gccagcgggt tcggcggaga ctggcgggtg cgccagcagc tgcccgtcgg agtcgggcct
     4201 gacgaccgga tgccgccagg cagccgggag ccgaagtggg cgcaataaaa gacagccaga
     4261 aacggtgaca gtgtcacgga atatcactcc tgttccctgc atacagccat acactacctt
     4321 ctgaccggaa agccgggccg gcagcgccgc aggcgctgca ccccgcgttt ccggcaggcg
     4381 tcagcctgcc tggggttttc gcgcgggctg acaggcggcc tccaccgcct gcccctgtat
     4441 cacctgtttc tgctgccctg aaggatgtag tgaccgccca ggaaaaccgc ctgctgattc
     4501 gccacaaaat cgcagatttc atcaacaatc tgcgcggtgg tgagcggttt ctgccccatt
     4561 tcacgacgcc gcgcattaat cacggccctg taattacgca gaatttcgcg ggtttctgtg
     4621 ctgatattca tgacatcgtc tccccgtcca gcgcctgtaa cacaacgtcc attgcttcgc
     4681 gttcctccct gacgaatgcg tcaccgtcgc ccccgttctc actcccgacg gcatacaggc
     4741 gcttcatttc actgcctgcc agcgccatca ctttttccag catcaccgcc gggactgtca
     4801 catgacggct ggcgcgcttt tcttccatca gttcacgaac aacgtcgtga acaacgctga
     4861 catccgcacc atgctcaaag gcatcgtcca gtcgctgcat gacggtttcc agctcatcgt
     4921 ctgacaggtt ccagtcctgg gctatgtgac gcacatcccc acgggtcagc accacggcaa
     4981 aaacgcattc actgtccggc acgtctgtat ccagtacatc gcggatgatt tctttaacag
     5041 tacagtacat gttctcgtct cctttacagg ggcggcattg ccgccccgtc cgttaatcag
     5101 tcaagtgccg cgtaaatcgc gttgcattcg gggtgaaatt cgatgtggct ccacaactgc
     5161 gcatcgcgca tcctgtacag gtgtgtcaac cctgcatccc cgctgtcgtg gtacagccac
     5221 aactggcggt taatcacaag ggaagtcaaa atgatgcctg cggcatcagc actcacggta
     5281 cggtcaaacc agttttcacc gttgaccaga tgaaaacggt cgccatccgg catcatgtaa
     5341 ccgccaccgt ccggcaggcg gatgaagtgc cagaatccgc cttcatattc cggcatcatt
     5401 tttttcgcca gcgcgtatac agacgcttcg gcatacagaa aatcgttgcc aaacaggtaa
     5461 ggcagaaaag aaaggcggtc gcactcttca acgttcagag tgacagattt cgcatactgc
     5521 atgggatttc ctccgttgtt cacatattcc gcgacaccgg gcggtgccgt gcctccatct
     5581 ttgccgggcc tgcagggttg caagggcgca agggaagccg cagcctgacg cacacggctg
     5641 acgggagttg gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc
     5701 agacgggaag gagggaccgg gtgaccgcag cggttcacct taccttgcaa cccgcccgca
     5761 gaagcccggc tacgatggag gcacggcaca gcccgcacac gacgctgagg ccacataact
     5821 gaaaacccac aaggccatgc cccgcatggc cgggtaaagc ggagcctgcg gcgacatcct
     5881 ttgtgctctg tccggggacc ggcaggaaaa ggcggagatt tttccccgcc agcgtcatca
     5941 tttccggatt tttgccaggg catccgccat gctgcggatt tcggcccggg cttcccggac
     6001 aacttcccgc agaacaccac ggtcaaacca ccggcggggc ggttcatccg gtatacgcag
     6061 cacatcgcag gccagcccac tggacaggat atgctcaata caatcgctga ccagatacat
     6121 ttccaccgac gaaaaacgag cgccccggtt caggatggca tcgagcacca cgggcaggtt
     6181 ttcgtcgtct tcatcgagcc agcaggggtg atcgctttcc ttcacaacca gacgaaagcg
     6241 gcgcgtctgg cgggtcagca acgtctgacc ggtggtagtc aacgccgcac ggatacgccg
     6301 cgcatcttct gccacccggc aactgacact gcatactgca tcgcgcaccg catcaagaac
     6361 tgccctgatt tcgtcttcgg tcagccattc tccggcatac gggaaagaca cctgcgttcc
     6421 gtctgccgga agtaccggca gcgactgcca ctgactgccg gacagaacaa gttccgcccc
     6481 ggttccggtc agtttctgaa tggcagaggc caggagcggc ggcagtacgc cgccgtgacg
     6541 gttgataagt aacgtcgcca gccggggcgc atccagccgc acgtaaatgc tgatccagcc
     6601 cggatacttc gggttccgct ggtcaacatc cgtttcttcc ggccagccct gcgccagcaa
     6661 caggttgctg gcgtggcgat aaatcagtgc attcagcgtt tcattcataa ttcatgaact
     6721 ccctgtatcg ccgcccgaag gcggcgataa catcagtcgt tttcgtgtgt gttgtttttc
     6781 agcagggtgc gggcctgatt aaccacctcc tgacggcggg tgacgcattc cgggacggcg
     6841 aggccatgat cccgggcggc ccattcacgt ttccacaatg cctgctcagc ggtctgaatc
     6901 agggacgcca gcagttcagc agggacggtc acctggcggt tttcgctcac gttattgatg
     6961 atctccatca ccgcagcgga agaaataccg gactcaatcc gctgttcttc cgggatgtcc
     7021 tccaggcgtg ccaggacggt cctgatttca tgaccggtca gagaaatttc cataccgtca
     7081 gccagtgcct gaatctcttc cggggaccag acaaccagca tcagcggcgt gttctccgta
     7141 tactgcgctg ccagctcacg gcagagcgtt tcgcatgttc cgtacattct cgtctccttc
     7201 tcaatcttta aagcacgcat ccagttccag aatttcggta tatgcctcca tcaggaggcg
     7261 gtttgcgcga aacaacgtct gacgcaccag acgttccggc agggtgtcag cattaaagct
     7321 gtaccgccgc tccatctcat tcatggttgt acgcaggtcg cacatggcat tggccacatc
     7381 cgccacttcg gcgcgaagtt tttcgtgtcc ggcatagttc atttacgcag cctccggcat
     7441 aaaccagtca tcattcgcgg ccccctgctg catggcccgt tgtacggcgg caaggcgttg
     7501 ctgcccggca cggtgatact gctcaagcag ctcgataccg atataccggc gtccggactg
     7561 gaaggcggcc acgcaggttg agccgctgcc tgcaaacggg tccagcacaa ttgcgttcgg
     7621 gtgtgtgaag ctctcaatca gcggttgcag gctggtaacg ggcttttccg tcgggtgatg
     7681 acgattgcct gaatatttcc agcccagcac gtccggcagc gggttttgtg gcagacgtgg
     7741 acggcctttt gccaggatgt aggcacattc gtggcgatag cccacatatg cggccttcga
     7801 tgtgtagttt ttggtgaaga ccaggtgacc aacaacgctg aatcccgcat ttttccaggc
     7861 agacataaaa cgatcgacgc gattccagcc atagaagctc accattaacg cgtctttttt
     7921 gagtacgcgg tacatctcat tacaggccgg ttgcagccat tcatcggttt tatcgccggc
     7981 aatggtacgc ccctgacggt cacgaaaacc gacgaggtac ggcgggtcgg tgaggatgaa
     8041 atcaatggcg ttatcaggga tacgggccat aacatcgatg cagttaccga ggacaaaacg
     8101 ggacatacca aactccttct cttacagagg gcaccgggag ggcacccctg tggtgcccgt
     8161 tccccgctgc ctgtgggctg ttcaccgcgt ccggtaacgg gtcaggccga ccgtggaata
     8221 ccgcagccgt cgtctttttg acggcaaggt tatgccgcga aagcggcttg aagggcgttc
     8281 atcagaaaag ggcgacgggc tggcgtacac tgtcaccggt gccagccggg agcggtaaaa
     8341 gcgcccgtcc cgtgcccggc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     8401 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     8461 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca ccttgtcgac
     8521 tatccacgca ggtccggtga cttccggcag ctcatcgtca tctgtcgggg aagaccattc
     8581 cagttccccc cacagcacat caacgagctc tccgcgctca tacaactgcc agccacagaa
     8641 attgcagccc tgttcggcgt accagtgctc cagcgtgcag ccgaaacggc ggcttaattc
     8701 tgcaacaacg tcgctttccg gctgacacca cggcgtgtcg aaatctacct gaatgaagtt
     8761 ttccccctga ctgctgatat tcaggtcata cccacaaggc cacttcacgc cataccgttc
     8821 tgtataccag tgatatgcag aaggaacacc gttcagaaca ccgccgttaa agccattcac
     8881 ttcaatatcc aggcgggtcg gcaaaaccgt cagcaggtca aacggcatgg ttttttccgg
     8941 cagtacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccagatgtc
     9001 acaccagtca cctctttttg cggtgaaatg cacagtgata gtttccctga catcatccgg
     9061 taactcttcc cagcgacgct gtccagtacc gctctgcagc cagagttcat gcagcatcag
     9121 gcagttttgc tcatccagca gtacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     9181 gatattttcc ggtgatacag caccacgtcc ggcagccgtc agtccggggc acggttcaaa
     9241 ccacacatct tcagtggtct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     9301 ttcatttgtg gcgcggcgat aaaacggtgt gactgcaccg ctggccagtc gtttaatctc
     9361 agcaatctgt gccggttcgg cagaaaaata catacggtta ctgcaccagt ttggcatggg
     9421 aattctcctc tcacttctgc ttcagcagag agcaccggga gggcgcccct gtgcgcccgt
     9481 tccccgctgc ctgtgggctg ttcaccgcgt ccggtaacgg gtcaggccga ccgtggaata
     9541 ccgcagccgt cgtctttttg acggcaaggt tatgccgcga aagcggcttg aagggcgttc
     9601 atcagaaaag ggcgacgggc tggcgtacac tgtcaccgac gccagccggg agcggtaaaa
     9661 gcgcccgtcc cgtgcccggc cggacgcggg acactgacca caggcagcgt tcctgccgcg
     9721 ccgcgtcagc cggcgcagta aaaagaggag cccggcgaca tcttttgtta tccgccaaac
     9781 gaacccaaaa acaacccata aaaacccaac aaaacaccaa aaacaagaca aatagtcatt
     9841 gattgatggt tataatgggg taaacttgac aaacaaaccc acttaaaacc caaaacacac
     9901 ccaaacacac accaaaaaaa acaccataag gagttttata aatgttggta ttcattgatg
     9961 acggttcaac aaacatcaaa ctgcagtggc aggaaagcga cggaacaatt aaacagcaca
    10021 ttagcccgaa cagcttcaaa cgcgagtggg cagtctcttt tggtgataaa aaggtcttta
    10081 actacacact gaacggcgaa cagtattcat ttgatccaat cagcccggat gctgtagtca
    10141 caaccaatat cgcatggcaa tacagcgacg ttaatgtcgt tgcggtgcat cacgccttac
    10201 tgaccagtgg tctgccggta agcgaagtgg atattgtttg cacacttcct ctgacagagt
    10261 attacgacag gaataaccaa cccaatacgg aaaatattga gcgtaagaaa gcaaacttcc
    10321 ggaaaaaaat tacattaaat ggtggggata cattcacaat aaaagatgta aaagtcatgc
    10381 ctgaatctat accagcaggt tatgaagttc tacaagaact ggatgagtta gattctttat
    10441 taattataga cctcgggggc accacattgg atatttctca ggtaatgggg aaattatcag
    10501 ggatcagtaa aatatacgga gactcatctc ttggtgtatc tctggtcaca tcggcagtaa
    10561 aagatgccct ttctcttgcg agaacaaaag gaagtagcta tcttgctgac gatataatta
    10621 ttcacagaaa agataacaac tatctgaagc aacgaattaa tgatgagaac aaaatatcaa
    10681 tagtcaccga agcaatgaat gaagcacttc gtaaacttga gcaacgtgta ttaaatacgc
    10741 tcaatgaatt ttctggttat acacatgtta tggttatagg cggtggcgca gaattaatat
    10801 gcgatgcagt aaaaaaacac tctcagattc gtgatgaacg ttttttcaaa accaataact
    10861 ctcaatatga tttagttaac ggtatgtatc tcataggtaa ttaataatgg acaagcgcag
    10921 aaccattgcc ttcaaactaa atccagatgt aaatcaaaca gataaaattg tttgtgatac
    10981 actggacagt atcccgcaag gggaacgcag ccgccttaac cgggccgcac tgacggcagg
    11041 tctggcctta tacagacaag atcccagggc ccctttcctt ttatgtgaac tgctgacgaa
    11101 agaaaccaca ttttcagata tcgtgaatat attgagatcg ctatttccaa aagagatggc
    11161 tgattttaat tcttcaacaa tcactcaccc ctcttcacaa caggagcaaa gaagtgatga
    11221 agagacaaaa aaaaacgcga tgaagttaat aaactgatta aatttttatt gataccttta
    11281 cccccataaa cggggtaaag gtgtatcaac aaacctactg gctgagtatt tctggtggca
    11341 gaactacgta agtagccgta cctgtatagt aatcatatat ccagagaaaa cataatgaaa
    11401 gatacaatta ctccactaaa ttacactaag gaagccataa aagacctgtc tgtcatggaa
    11461 aaaggattaa ggtcaaatgc acctcttaca tcatatatta tgcgtcaggt cattgatatg
    11521 ctggacagta gtgttaagtt catcttaccg aactgctgcg atattattga accagaagaa
    11581 taccgccaga cacatttcga tttggcacga ttaccatatc cagttgtaac atttgaaata
    11641 ccatggttca aagacagtgt agaaacccaa ataggtgatt ttaatatatc accatcatcc
    11701 agaagaattg ccctgtgctg ggaggccaga cagagttttg aacccatccc gggatgtaat
    11761 tcaatcctta acacttatat tgatggcgga gtgtttatcc ttcctgtttc atggtctgat
    11821 gatttaaaaa tatggattct tggtgtcggt ggaatgtttt tcccatataa caacaaactc
    11881 actaaatacg aacctgacag gacattgcct gcatccaggc ttgtaatcga cacattaaaa
    11941 gagaatggag tagccaaata taatgcagca cacttcaaag cagagccttt tataacaagc
    12001 atggaattta aagatgattt gataaagcag gtgggaagta ttgaaagatt atatgcgcaa
    12061 attattatgg atactcgcga tgaactacag gctttcatac aggcatgtag cgtgttaaat
    12121 tgcgaaaatg tgtgtcctgt cacacttagc actaaaccag aaagaaaatt cataaatggt
    12181 cgtaaagttc agccacctga aaaaaacaag cgcccttcat atacttataa agtattgcaa
    12241 ctgtctgaaa ctaaggtgca aagtaatcat actggaacag gaaagtccgg tgggacgaaa
    12301 aggatgcact tacgcagagg acatattcgc aggaaaaata acaaactaat atggatcaga
    12361 ccagcaatga ttaatgcaaa cagtagagca ggcattgttg ataaggacta ccagataaat
    12421 ataagaaaag aagaaaacaa accttagtaa atattactgt gtaagtaaca atccgttgta
    12481 tcctgctata aacgttcctg attcacatag cagggtacag agtcatcttc gtaaa
//
LOCUS       DAZFRJ010000132        12460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000132 DAZFRJ010000000
VERSION     DAZFRJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.133"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(148..1572)
                     /gene="patD"
                     /locus_tag="ACHKUN_003348"
     CDS             complement(148..1572)
                     /gene="patD"
                     /locus_tag="ACHKUN_003348"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO6661556.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1594..2388)
                     /gene="ydcV"
                     /locus_tag="ACHKUN_003349"
     CDS             complement(1594..2388)
                     /gene="ydcV"
                     /locus_tag="ACHKUN_003349"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6661557.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2378..3319)
                     /gene="ydcU"
                     /locus_tag="ACHKUN_003350"
     CDS             complement(2378..3319)
                     /gene="ydcU"
                     /locus_tag="ACHKUN_003350"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6661558.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3320..4333)
                     /gene="ydcT"
                     /locus_tag="ACHKUN_003351"
     CDS             complement(3320..4333)
                     /gene="ydcT"
                     /locus_tag="ACHKUN_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6661559.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4351..5496)
                     /gene="ydcS"
                     /locus_tag="ACHKUN_003352"
     CDS             complement(4351..5496)
                     /gene="ydcS"
                     /locus_tag="ACHKUN_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO6661560.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5741..7146)
                     /gene="ydcR"
                     /locus_tag="ACHKUN_003353"
                     /pseudo
     CDS             complement(5741..7146)
                     /gene="ydcR"
                     /locus_tag="ACHKUN_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7225..7641)
                     /gene="hicB"
                     /locus_tag="ACHKUN_003354"
     CDS             complement(7225..7641)
                     /gene="hicB"
                     /locus_tag="ACHKUN_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO6661561.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7687..7863)
                     /gene="hicA"
                     /locus_tag="ACHKUN_003355"
     CDS             complement(7687..7863)
                     /gene="hicA"
                     /locus_tag="ACHKUN_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO6661562.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8085..8315
                     /gene="yncJ"
                     /locus_tag="ACHKUN_003356"
     CDS             8085..8315
                     /gene="yncJ"
                     /locus_tag="ACHKUN_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO6661563.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8407..10368)
                     /gene="rlhA"
                     /locus_tag="ACHKUN_003357"
     CDS             complement(8407..10368)
                     /gene="rlhA"
                     /locus_tag="ACHKUN_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO6661564.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10441..10977)
                     /gene="sutR"
                     /locus_tag="ACHKUN_003358"
     CDS             complement(10441..10977)
                     /gene="sutR"
                     /locus_tag="ACHKUN_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO6661565.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11030..12244
                     /gene="ydcO"
                     /locus_tag="ACHKUN_003359"
     CDS             11030..12244
                     /gene="ydcO"
                     /locus_tag="ACHKUN_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO6661566.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12284..>12460)
                     /locus_tag="ACHKUN_003360"
     CDS             complement(12284..>12460)
                     /locus_tag="ACHKUN_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6661567.1"
                     /translation="RLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3048 a         3345 c         3056 g         3011 t
ORIGIN      
        1 caactgtgac aggatgaaaa atcgccatca aacatttagc ctaattttta cgctagttat
       61 taatgtaatc attctaatac ttaaccgcca ttaaactcag aacctaaaac gatagtggcc
      121 gtcctataac agccactaac atctaactta atgtttaacc atgacgtggc ggacgacggt
      181 gtaatcctcc agcccataaa gtgacatatc cttgccgtaa ccagaaagtt tctgcccacc
      241 gtgcggcatt tcacttacca gcatgaaatg ggtattgacc caggtacaac catattgcag
      301 ccgtgcgctg acgcgatgcg ccctgcccac atctttcgtc cataccgaag atgcaagtcc
      361 gtactggctg tcattcgccc agttcaccac ctgttcttcg ttgtcgaagg gcgtaacact
      421 cactactgga ccaaatacct ctttttgcac gatggcatcg tcctgtaatg cgccagccag
      481 cagcgtcggc gcatagtaat agccattacc cttgcgcttt tcaccgccag tgatcacttt
      541 gatgtgccct gtcgctttcg cctcttctac tgccttgctg acgcgttcga gatgcgccag
      601 cgagcttaaa ggtccaagct ccgtagactc gtcatccggc gcaccagatt ttaacgttgc
      661 caccgcagca cccagttttt ccaccagcgt atcgtaaatg cctttttgcg cgtagatccg
      721 acaagccgca gtacaatcct gtccagcatt gtaatagcca aatgtacgta caccttcgac
      781 cactgcttca atatccgcat catcaaaaac aatcactggc gctttgccac caagttccat
      841 atgagtacgc ttaatggacg acgcggtatg gctgatgatg tgctcgccgg tggcgataga
      901 gcccgtcagc gacaccatcc gcactttggg atgaccggtc agcggatcac ccaccgtttt
      961 gcctctgcca aacagtacgt taatcacgcc tgccgggaag atatctttcg ccagctctgc
     1021 caacttcaac gcggtcagcg gggtaatttc tgatggttta agcactacgc agttccctgc
     1081 cgccagcgcc ggagccagtt tccacgctgc catcatcagc ggataattcc acggtgcgat
     1141 agaagccacg acccccaacg gatcgcgacg gatcatcgaa gtatgacctt caagatattc
     1201 acctgccgcc agaccattca gacagcgcgc cgcacccgcg aaaaagcgaa aaacatcgac
     1261 aatcgccggg atttcatcat tgaacgcact atgcagcggt ttgccacaat tacgggactc
     1321 cagttcggca aaaacctgac cattttcttc gataacatca gccagtttca gcagacattc
     1381 cgcacgcact ttcggcgtgg tttgccccca ttcggcaaat gctgcatctg ccgcgcgcac
     1441 agcagcatcg acctgctctg cggatgcctc ggcaatctcc agtaaaacgt cccccgttgc
     1501 cggattatag acaggctgtt tttccccttc gccgctaacc agttctccgt taatcagtaa
     1561 cttatgttgc atagcatatt cctgtatcag tggttattga ccattgtcgc cttcgcgggt
     1621 tagccaccag gcccccagga tcggcaaggt tgttaccaac ataaccagca gtgccaccac
     1681 gttagttacc ggtacatcac gcggtcgccc aagctgattg agcaaccaca acggtaacgt
     1741 tcgttcatga cctgccgtaa aagtcgtaac gatgatttca tcgaacgaca aggcaaacgc
     1801 cagcattcct cctgccagta gcgccgaact gagattcggc aacactacgt agcggaaggt
     1861 ttgccagcca ttggccccaa gatccattga cgcctcaacc agactccacg aggtgcggcg
     1921 aaaacgggcg atgacattgt taaacaccac cactacgcaa aaagtcgcat gaccgaccac
     1981 gatggtgaaa aatcccggct ccagattgat ggttttaaaa gcggttaata gcgccagacc
     2041 agtgacaatg cccggcagcg caatgggcag cagcagtaac agcgaaatgg cgtttttgcc
     2101 gaaaaagtct cgtcgccaca gcgcggcagc tgccagcgtc cctaacacca gcgccattaa
     2161 tgtcgccagc gccgccactt taagtgacag tgtcacggca tcaagaatat cactacgctg
     2221 tgctgccacg ctaaaccagc gcagcgtcag gccctgcggt ggaaaactaa acgccgcatc
     2281 ttcagtgtta aacgcataag cggcgataat caggatggga aaatgtagga aaaccacgcc
     2341 gccccaggcc gccagtttga ggaaaaacgg tgcgcgttca gagtgcatcg aacgctccca
     2401 gacgtttcac gaacgccagg tacagtgcga tgagaacaat cggcaccagg gtgaatgccg
     2461 ccgccatcgg catattgcca atcgccccct gctgggaata aaccatattg ccgataaaat
     2521 atcctggagg accaaccagc tgcgggacga taaaatcgcc cagtgtgagt gagaaggtaa
     2581 agatagagcc agcggcaata cccgggattg ccagcggcag caccacatag cgaaaggttt
     2641 gtcgtggacg tgcgccgaga tcagccgacg cctgcaacaa tgacggcggc aaacgctcaa
     2701 gcgccgcctg aacgggcagg atcatgaacg gcaaccagat atagagaaac accagaaagc
     2761 gccccagccc ggaagttgac agcgtatttc cgcccaccgc aggtaatgta aggaacgcag
     2821 tcagcagtgg ttccagccca agatgttgta aaaaccactg agccacgcca tcttttgcca
     2881 gcaataacgt ccaggcatag gctttaacaa tgtagctcgc ccacatcggc aacattaccg
     2941 caatataaaa aaaagctttc attttcccgc tggtatagcg cgccatatac cacgccattg
     3001 gaaaagccag aatggcgctg gcgatagtga ctgccacagc catagtcagc gtgcggagaa
     3061 tgatgtcgta attcgccgga ttaaacagcg cacggatatt cgccagcgtc agttccggcg
     3121 ttaccgacat ggtgaaatcg tcaaaagtat aaaatccctg ccataacagt gtcagcagcg
     3181 agccgaaata gacaatgcca aaccacatta gcgggccaag cagcagtaaa aacagcccca
     3241 gcctcggatt acgccagaag aaaccggaca ccttacccag acctggacgt gaaggtgatt
     3301 gcaatacatt catcgccatt cacctctcct caaccagcgg caccatcaca tcacgcgacc
     3361 aggaaaccat cacctgttgt ccgggcgtga gcgtggcagg cagttcttcg cctgtcatat
     3421 tggcctgact cacaagcagt ttttcaccgc cgttcaattt cagttcaaaa cgagttgccg
     3481 cgccctgata ttgcaccgcc tggatcgtgc cattggcctg cagttcacca ggggtgttga
     3541 ggcggatatg ttccggtcgc agggcgaagc ttcccgtcat gccacaaagt ttctctgcca
     3601 tcagtccatc aaaaacgttg gatgtcccga cgaatccggc aacaaacggc gtgcgcgggc
     3661 gcatatagag atcgcgcggg gaatcgacct gctcaatgcg tccattattg aaaaccgcca
     3721 cacgatcgga catcgataac gcttcgccct gatcgtgggt aacgaagata aaagtgatac
     3781 cgagagactg ttgcagtttt ttcagttcca gctgcatctg ctcacgcaat ttgagatcca
     3841 gtgcgccgag cggttcatcc aacagcaata cgcgcggttc attcaccaat gctctggcga
     3901 tagcaacccg ctggcgctga ccaccagaaa gttgtgacgg tttacgttga tgtacaaacc
     3961 ccaacgccac tttctccagc gcctcttgcg ccattgcgtg ccgctgcttt ttattcacgc
     4021 ctttgaccat cagcccatag gcgacattgt caagaatcga catatgcgga aatagcgcgt
     4081 agtcctgaaa gacagtattc acgtcccgct cccacggtgg cagattgctg gcgggtttac
     4141 caaagataga gatagccccg ccggaaagct gttcgaagcc agcaatcagg cgcaggcagg
     4201 tggttttgcc ggagccggac ggccccagca tagagaagaa ctcaccatct tttatcgcaa
     4261 tactgacgcc atctactgcg cgcacgtcac cgtacaaccg cgagacgttg tcaaactcca
     4321 ctgcgtacgt cataaagcac cccagcgaag ttagcgaccg cccataatgg caatgtaatc
     4381 ctgcgtccag cgactgtagg gaacaaactt gcccccttct gctataggcg ttttccagaa
     4441 ggcgattttg tcgaaatagt taaaaccgtt ggtttcacaa cctttttcgc ctaataacgg
     4501 actggcttta cacccttccg gcactaccgg taacgagcca aaccaggccg ccacatcgcc
     4561 ctgcactttt ggcgttaatg accagttcat ccatttgtag gcgcaaaccg gatgtttcgc
     4621 ttcgctatgc agcatggtgg tatcagccca accggtaaca ccctccttcg ggaaaacggt
     4681 agcaacaggc tggccttcgg ctttcagggc gttggcctga tagggccagg cactggaagc
     4741 aaccacaccc tcgtttttga aatcgctcat ttgcacggta gtgtcatgcc agtagcgatg
     4801 gatcaaactg tgttgagcgc gcagcacttt cagcaccgcc tggtactgtt cttcggttag
     4861 ttgatacgga tcgctgatgc ccaactgcgg ctgagtggct ttaacgaaca acgcagcgtc
     4921 cgcaatgtag atagggccat cataagcctg aacgcggcct ttattgctct tgccgtccgg
     4981 cagattttgc tcaacaaaaa ccacttgcca gctatccggc ggcgtcggga aggttttagt
     5041 gttgtacatc agcaggttcg gcccccattg gtaaggtgtg ccgtaaactt tgccgccaac
     5101 attaaaccag tcgcctttaa ccacgcgcgg atcgagcgtt ttccagttgg gaatcaaggc
     5161 ggtattaatc ggctgcacgc gtttgcccat aatcaaacgc agcgaggcat cgccggatgc
     5221 cgtaaccaga tcgtaacccc ctttggtcat cagactgacc atttcatcgg aagtcgcggc
     5281 ggttttcaca ttcaccgcgc agcctgtctc tttttcaaat tgcgttaccc agtcgtattg
     5341 tttatcagtt tgtccgcgtt cgatgtatcc cggccaggcg ataatatcca gtcgcccttc
     5401 cggtttatct aaattggtag gtggttcggc ggcgtgagcg gtcattattg tcatggtgag
     5461 cgcacacagg ctgctgcggg caaatgtctt gctcataagg tcttactcct gtcgtaatta
     5521 attgttcagc cgccgtgccg taaaaatatc tccttgttta accatagacg gcggcttgca
     5581 ggcacacctt cgcccctgag aaaatttaat caggttgtga gctaccgcgc attatgccgt
     5641 ctgtaaggtt atcttctgga gtatagacaa ggggttagga aatatgactg ctattcgaaa
     5701 ttcatattac ccaatctaag aggaattaaa ttaaacgcta ttacagccgt tcttgaataa
     5761 gtttgcctaa ttgttttacc gcctgttctt cacgttctcc ccactgccac gcggtattaa
     5821 aacgaaaaaa acgtgaccag ttttcaccgg tagaaaacat tttacccggc gcaatactga
     5881 tatgatgcgt cagtgccgcc aggcttaatt cgccggcatc taacggctcg gggagctcca
     5941 gccagagaaa ataaccgctg tcactatgat gaattttaac ttccgcaggc agataacgca
     6001 gcagtgcctg ccaggcacgt tgtttacgtt ccgcaagctg gcgacgcagg cgacgaagat
     6061 gggcgtcgta tcggcgcgtg gaaaggtaat ccaccagcgc aagctgcatc ggtgagctgg
     6121 tggaaagcgt actcatcaac tgcaagcgtt gaattttacg tgcatgtttt ccggcggcga
     6181 cccaaccaat acgaaaacca ggcaccagac atttcgaaaa cgaagagcaa tgcaaaacgc
     6241 catcgtggcg atcccacgct ttcgcaggca gcggtttttc ccgtccaaaa taaagttcgc
     6301 tgtaaacgtc atcttcaatc agcgttacgt tgtactgatt gagtaacgcc accagttgtg
     6361 ctttttttgc ggcgttaagg taaatccgag tggattctgg ctattagtca tcagccagca
     6421 cgctttcacc ggatactcct gcaacgccag ttccaacgcc tgaagatcta tcccttcttt
     6481 aacatccgtc gccaccgata acgctttcag ccgtagccgt tccagcgcct gcaacgcgcc
     6541 atagaaacaa ggattctcta ctatcaccca atcgcccggt tcagttaccg cttgcaaact
     6601 gaggtttaat gcctctaacg ccccggcagt aatgacaatt tcatcaggag aaatggtgat
     6661 gccctgtaag gcatagcgac gagcaatagc ctgacgcagt tctgcgtttc ctggcggtaa
     6721 gttttcaatc acgctcatcg ccgtggcggt tttgcttacc tgcgccagcg agcggtttag
     6781 ttgttggagg gggaaaagtc gcgggtcggg aaaggccgag gcaaacggaa cgaccgacgg
     6841 atcgcgactg gcctgcaaca tatcaaaaat ataagtgttg atatcgactg cttcatctcg
     6901 agtgactgga atgactggcg ctttcggcat ttttattgcc tgtggcgcaa cgtaataacc
     6961 cgactgcggt cgtgcgataa tatatccctg actttcgagc aactgatagg catggctgac
     7021 agtcataaag ctcatgcctg aaagcgccac ctggtcacgc aacgaaggca aacgatcgcc
     7081 gggttgccag atacccgacg caatctgctc gcgtaattgt tctgcaagct gctggtattt
     7141 tttcattatc gaatcgtaat tatgtgcaga tgattcggca gtctatatca gttgttaaaa
     7201 atgacaactt ttcgttaact gtaattaaac catcaccagc gataactctt tgccaagcgc
     7261 ctttgccgca agctggacgg cgtcgatttt tgtcgcatgg tgcaagttaa ataggcgagt
     7321 aatctcctgt ttaggtttgc caattcgcct ggctaactct tgctgagtga tttctgactg
     7381 taaaaaagca tttaacagca atactttaga ggcgacgctc aaaggtactt caataaagtg
     7441 atcatgacta tttaatggcg aaggtaacgg gataagctcg ttatcttcaa aataaaaatc
     7501 aaatgcggtc agtaaagcat cttttgccgc ttccatcgct tcagcgacag tttcgccctg
     7561 cgtcaacgct tcagggatat ccacaaaaga aaccatataa ccgccttccg gcgcgggtgt
     7621 aagagtgacg ggataacgca taatttcgac tgtccctcgc atgtcattaa cctttttaat
     7681 tggcgattaa ctcaaaccga gttgtttcag gattgcttta cgcaatggtt ctttaatctc
     7741 atcgcagggg tgacgcggca tgacactgcg cctcccatga aacctgagtt tcaaatggtt
     7801 gctgccattc gctacatcga cgccctgaga ttcgagccaa cgtctgaact cgctttgttt
     7861 cacatcgcct ccctgccgct gagtatatca acaaagtaaa cattagtgtt tacctccgtg
     7921 tgtaatttgc tcgaaaatag cactaaatcg ccgcaacggc agaatgccat gcaaaatcag
     7981 gaagaaagac tccagaattt ctttgcgcta atttgcaaaa ctgaataata acctcaagaa
     8041 acgatcggca tcatagcggc aaaaggtaag aaaaaggtgg caggatgttt acgaaggcgt
     8101 tatcggttgt cttattaacg tgtgcgctgt tctcaggaca actcatggca gggcacaaag
     8161 gacatgaatt tgtgtgggta aagaatgtgg atcatcagct gcgtcatgaa gcggacagcg
     8221 atgaattgcg tgctgtggcg gaagagtcgg cggaaggttt gcgcgagcat ttttactggc
     8281 aaaaatcgcg caaaccagaa gcgggacaac gttgagtcat ttgttgccgg atgcggcgag
     8341 acgccttacc cggcaggcaa tatcatgcag attcaatgag ttgtgagtaa ccagaaaact
     8401 ggcgttttac ccttttcgct taggcaatgt tttcagcagc tcatccggac ttacggatgc
     8461 cactacgctt cccggtaacg gcattttcag tatgtgattt ttgattttgc caatgacgtg
     8521 catctcgcat gggcggcaat caaactttag cgttaatact tcatcgccgt taaccagttg
     8581 cattggcgtc gccttccagc ttttgatatt gcctttcgcc tgtttcgggc acagattaaa
     8641 ggcaaagcgc agacaatgct tggtgatcat caccgggact tcgcccttct cttcatgtgc
     8701 ttcatacgcc gcgtcaatca gctgcacacc atagcgatga taaaattcac gcgctttctg
     8761 gttgtatacg ttcgcgaaga aactcagatg cgtttgcgga taaaccggcg caggatcagc
     8821 aaccggttta cggctgccgc gctggtaact ggcaagacgc gcagcatcca gcatgtcagc
     8881 agcttcacgg cggaactggt ttaacagact gtttggtaca aacagcgccc ccggcaaatt
     8941 aatttgcaca tcgcgggcgt aatagagggt ttgccccagt tttgccagac catccttcag
     9001 attgttcatt gctttttcgg cgttattggc ttcgtcgaac tgcccgtcca gcgtatgcgt
     9061 gatgctgaca ccctcttcac tggtgagggt cagaatcagt tgttcctgcc agccgcccag
     9121 ttcaatgtct accgccaccc gacgttcgct ggaggttttt gtcagtgcct gctgccagtt
     9181 atgatcaaga ttacggttta gtgggtgatg tggacgaatt ttgtgcaaat ctgctggcat
     9241 ttcattgggc cagacgcggt actgattttc tccggttttc tcgaccgtat tggcacgaaa
     9301 accgacgact tcacgtttaa tcatcacgtt caggccatca ccatttgcca gtggctcggt
     9361 gacggcaaca tcgagatgat ctttcgccac tttcaatact tcgcctaccg gcaggccgat
     9421 aaatttcggc gaatcgaatg cgccaatatc gcctttacgg gcattcacaa aataatctgt
     9481 gctaccacgg tggaaagtct tttccgtcga tggaacaaag aaatgttcag tacgacctga
     9541 tgaagcgcgc gccagatcgc cacgttcttc aataatggca tcaagcatct ggcgataatg
     9601 ggcggtgata ttcttcacgt agctcatatc tttgtaacgc ccttcaatct tgaaggagcg
     9661 tacgccagca tcaatcagcg cgccgaggtt ggcagtctga tcgttatctt tcatcgacag
     9721 cagatgtttt tcataggaaa ccacccgccc ctgatcgtct ttcaatgtgt atggcaaacg
     9781 gcacgcctgc gagcaatcac cacggttggc gctacgccct gtttgcgcat gagaaatgta
     9841 gcactgaccc gaataggcca cgcacagtgc cccatgaata aagaattcaa tggtcgcgtc
     9901 cgtggcctgg tgaatcgcgc ggatctgctc aagattcagc tctcgcgcca gcacaatctg
     9961 cgtgaagcca acatcagaga ggaacttcgc tttttctact gtacgaatgt cgcactgcgt
    10021 actggcgtgc agttcaatcg gcggaatatc aagttccaga atccccatat cctgaacaat
    10081 cagcgcatcg acaccggtct ggtagaggtc ggtaatcagc cgttgcgcgg gttccagctc
    10141 atcatcatgc aaaatggtgt taagcgtgac gaaaattttt gcaccataac gatgggcaaa
    10201 cggcaccagc tcggcaatat ctttcaggct attactggca ttatgacggg caccaaaacc
    10261 agggccgccg atataaacag catcggcacc gtgcaaaata gcttcgcggg caatggcggc
    10321 atcgcgtgcc gggcttaaca gttcaagtcg atgagaagat acggtcacat tcttatccag
    10381 tagctgaaaa atggcggcta ttttagccac aagcgcgacg ttgcgaaata gttttcccgc
    10441 ttaactgcgc gggtaatgga tgagggaatg aaaatgtacg gtttgctccc caccattacg
    10501 atagatatgc gtgacgtctg cagcaaatcg aacgccttcc ccacaattaa gggtctgcca
    10561 ttccccatca acgcacagat caagttgtcc atcaatgacg acgacatgtt cgattacccc
    10621 cttctcatgg ggcgtcgatt cgcttattgc accagatgcc atctgaatcg agaagtgttc
    10681 gaagcagagt tgtggatcat aaggaaacag cgaagtgatc accatcgcct gctgttgtgg
    10741 atcataaact gaaggcgtag cggactgcgg cggagaaata aatgttgaaa atggtacgtt
    10801 caacccggtg gcaattttcc ataatgtcgc gaccgtcgga ctggactcat tacgctcaat
    10861 ctgccctaac attgctttag aaacgcccgt cgcttctgcc agtcgcgaaa gactccagcc
    10921 gcgctgctgg cgtagttgtt taagtgtggt ggataaaaag cgagcgagat tttccatgcg
    10981 actcctccgg caaaacggaa gtttatcact tgtgcgttat aacggacaaa tgctacggtg
    11041 cctgtacgct ataacgcacg aggtgactat gcgtctgttt tctattcctc cacccacgct
    11101 actggcgggg tttctggcgg tattaattgg ctacgccagt tcagcggcaa taatctggca
    11161 agcagcgatt gtcgccggag ccaccactgc acaaatctct ggctggatga cggcgctggg
    11221 gctggcaatg ggcgtcagta cgctgactct gacattatgg tatcgcgtac ctgttctcac
    11281 cgcatggtca acgcctggcg cggctttgtt ggtcaccgga ttgcagggac taacacttaa
    11341 cgaagccatc ggcgttttta ttgtcaccaa cgcgttaata gtcctctgcg gcataacggg
    11401 actctttgct cgtctgatgc gcattattcc gcactcgctt gcggcggcaa tgcttgccgg
    11461 gattttatta cgctttggtt tacaggcgtt tgccagtctg gacggtcaat ttacgttgtg
    11521 tggaagtatg ttgctggtat ggctggcaac caaggccgtt gcgccgcgct atgcggtaat
    11581 tgccgcgatg attattggga tcgtgatcgt tatcgcgcaa ggtgacgttg tcacaactga
    11641 tgttgtcttt aaacccgttc tccccactta tattacccct gatttttcgt ttgctcacag
    11701 cctgagcgtt gcactccccc tttttctggt gacgatggca tcgcaaaacg caccgggtat
    11761 cgcagcaatg aaagcagccg ggtattcggc tcctgtttcg ccattaattg tatttactgg
    11821 attgctggca ctggtttttt cccctttcgg cgtttattcc gtcggtattg cggcaatcac
    11881 cgcggctatt tgccaaagcc cggaagcgca tccggataaa gatcaacgtt ggctggcagc
    11941 tgccgttgca ggcatttttt atttgatcgc aggtctgttt ggtagtgcta ttaccgggat
    12001 gatggctgcc ctgcccgtaa gttggatcca gatgctggca ggtctggcgc tgttaagtac
    12061 cattagcggc agtttgtatc aggcgctgca taatgagcgt gagcgcgacg cggcggtggt
    12121 ggcatttctg gtaacggcaa gtggattgac gctggtcggg attggttctg cgttctgggg
    12181 attaattgcc ggaggcgttt gttacgtggt gttgaattta atcgctgaca gaaaccgata
    12241 ttgacatcct ccacgccctg aaggacgagg atccctgcta cgttcaggct gtcgcctgaa
    12301 tcatttcggt gggttcctgc ttcaacgggc ggcctgactg caccatctct ccacaggcaa
    12361 gaacggcgtg ccccgccgct aaaatgttac gagcgccatt tacatcggcg ttcgctgtat
    12421 atccacatac ctggcatctg aattgacttt gtgacaggcg
//
LOCUS       DAZFRJ010000133        12352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000133 DAZFRJ010000000
VERSION     DAZFRJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.134"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..273)
                     /locus_tag="ACHKUN_003361"
     CDS             complement(<1..273)
                     /locus_tag="ACHKUN_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO6661568.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNR"
     gene            complement(536..1645)
                     /gene="apbC"
                     /locus_tag="ACHKUN_003362"
     CDS             complement(536..1645)
                     /gene="apbC"
                     /locus_tag="ACHKUN_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO6661569.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1777..3810
                     /gene="metG"
                     /locus_tag="ACHKUN_003363"
     CDS             1777..3810
                     /gene="metG"
                     /locus_tag="ACHKUN_003363"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO6661570.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3951..7745
                     /locus_tag="ACHKUN_003364"
                     /pseudo
     CDS             3951..7745
                     /locus_tag="ACHKUN_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7755..11387
                     /gene="yehI"
                     /locus_tag="ACHKUN_003365"
     CDS             7755..11387
                     /gene="yehI"
                     /locus_tag="ACHKUN_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO6661571.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11449..11766
                     /gene="yehK"
                     /locus_tag="ACHKUN_003366"
     CDS             11449..11766
                     /gene="yehK"
                     /locus_tag="ACHKUN_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO6661572.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3214 a         3156 c         3036 g         2946 t
ORIGIN      
        1 gcgatttaac acgattcgac gtttgctatc gctacctgca acaaccactt ctgtcgttac
       61 acctttgacg ggcgaagaaa acagatgacg ggtgtcaaca ttatacgtta actgaccaca
      121 atgctgttga ggttgaccat taaccatagc catactgcat gttgggggac tgatcctgcc
      181 attaatcgct aatgtggcag tctctgaaga gaatgcggga gaagccaatc cataggcaag
      241 aaaaataatt cctgacagcc aatacttatt cattgaacgt tatccctgta gtaaaggtta
      301 tgcctggata gaatgagtgc ataacaaact atagctgtac atccactaca cagccacgaa
      361 ggatgataat gaagcattgc ctgtatgatc aatcgacttt gtagagtttc ggacgaaggt
      421 ccgcagaata ttcgcagtat taaataagtc ttcattaatg cggcaaaaac taatacaccg
      481 catcaatgta acatctctgc ggtgtatgaa gaataaccag aatggttaat aggcgttaga
      541 ccgcgcggaa ggaaatctcg cctggaatga cttcaccctg ccagtagagc tgagctgcaa
      601 cgcggtcagc cagctggcgg tagatagcgg taaattcgct ctccggacgg ctgataaccg
      661 ttggcgtgcc tttatcgaga tcctcacgta aggagatgtg taacggcatc tgccccagca
      721 gctgtgtgtg gtatttttca gccagtttct cagcaccacc ggtaccgaaa atcggttcat
      781 ggtgaccaca gttactgcaa atatgcacac tcatgttctc gacaatacca agcaccggaa
      841 cctcgacttt ctcgaacatc acaatgcctt ttttcgcatc gatcaacgcg atgtcctgcg
      901 gggtggtgac cacgactgca ccagttactg gaatgttttg cgccagcgtc aattgaatgt
      961 caccggtgcc tggcggcata tcaagcacca gatagtcgag atccggccac aaggtttcct
     1021 gcaacatctg cattaacgcc ttgctcgcca tcggtccacg ccacaccatc gcattgtcgt
     1081 cagtgaccag ataaccaatt gaattggttg ccaggccatg agacataatc ggtgccatgt
     1141 gggtaccgtc cggcgacgtt ggacgctgat tttctgcgcc cagcatggtg ggaattgatg
     1201 ggccgtagat atcggcgtcc agaatgccga ctttcgcccc ttccgccgcc agtgccagcg
     1261 ccaggtttac cgccgtggag gatttaccca cgccgccttt accggaactg accgcaatga
     1321 tgttcttcac accattaata cccggctgat tcttcacgcg ttttaaggtg gcgatattgt
     1381 gcgacagttt ccagtcgata gctttcgccc cggtgatacg cagcagttcg gcgctacatt
     1441 gctcttttaa ctcttcaaac gcgctatgcc agacgaatgg cataaccagt tccacatgca
     1501 acgtgtcgtc catccaggcg acatggtgca acgctttcag cgtggtgagg ttatgcttca
     1561 gggttgggtg ctgaaaattg gccagtgtcc cggcgaccat tgcgcgcaag gcttctggtg
     1621 atttggcctg ggattgttcg ttcatcccga ctcctttttt gtatagataa accatcagct
     1681 gatagtttac ctgaagaata tagagaagta cttacttaac attttcccat ttggtactat
     1741 ctaacccctt ttcactatta agaagtaatg cctactatga ctcaagtcgc gaagaaaatt
     1801 ctggtgacgt gcgcactgcc gtacgctaac ggctcaatcc acctcggcca tatgctggag
     1861 cacatccagg ctgatgtctg ggtccgttac cagcgaatgc gcggccacga ggtcaacttc
     1921 atctgcgccg acgatgccca cggtacaccg atcatgctga aagctcagca gctcggtatc
     1981 accccggagc agatgattgg cgaaatgagt caggagcatc agactgattt cgcaggcttt
     2041 aacatcagct atgacaacta tcactcgacg cacagcgaag agaaccgcca gttgtcagaa
     2101 cttatctact ctcgcctgaa agaaaacggt tttattaaaa accgcaccat ctctcagctg
     2161 tacgatccgg aaaaaggcat gttcctgccg gaccgttttg tgaaaggcac ctgcccgaaa
     2221 tgtaaatccc cggatcaata cggcgataac tgcgaagtct gcggcgcgac ctacagcccg
     2281 actgaattga tcgagccgaa atcggtggtt tctggcgcta cgccggtaat gcgtgattct
     2341 gaacacttct tctttgatct gccctctttc agcgaaatgt tgcaggcatg gacccgcagc
     2401 ggtgcgttgc aggagcaggt ggcaaataaa atgcaggagt ggtttgaatc cggcctgcaa
     2461 cagtgggata tctcccgcga cgcgccatac tttggttttg aaattccgaa cgcgccgggc
     2521 aaatatttct acgtctggct ggacgcaccg attggctaca tgggttcttt caagaatctg
     2581 tgcgacaagc gcggcgacag cgtaagcttc gatgaatact ggaagaaaga ctccaccgcc
     2641 gagctgtacc acttcatcgg taaagatatt gtttacttcc acagcctgtt ctggcctgcc
     2701 atgctggaag gcagcaactt ccgcaagccg accaacctgt ttgttcatgg ctatgtgacg
     2761 gtgaacggcg caaagatgtc caagtctcgc ggcaccttta ttaaagccag cacctggctg
     2821 aatcattttg acgctgacag cctgcgttac tactacactg cgaaactctc ttcgcgcatt
     2881 gatgatatcg atctcaacct ggaggatttc gttcagcgtg tgaatgccga tatcgtgaac
     2941 aaagtggtta acctggcctc tcgtaatgcg ggctttatca acaagcgttt tgacggcgtg
     3001 ctggcaagcg aactggctga cccgcagttg tacaaaacct tcactgatgc cgctgaagtg
     3061 attggtgaag cgtgggaaag ccgcgaattt ggtaaagcag tgcgcgaaat catggcgctg
     3121 gctgatctgg ctaaccgcta tgtcgacgag caggctccgt gggtggtggc aaaacaggaa
     3181 ggccgcgatg ccgacctgca ggcaatttgc tcaatgggca taaacctgtt ccgcgtgctg
     3241 atgacttacc tgaagccggt actgccgaaa ctgaccgagc gcgcagaagc attcctcaat
     3301 acagaactga cctgggatgg tatccagcaa ccgctgctgg gccacaaagt gaatccgttc
     3361 aaagcgctgt ataaccgcat cgatatgaag caggttgaag cactggtgga agcgtctaaa
     3421 gaagaagtga aagcagctgc cgcgccagtt actggcccac tggcagatga tccgattcag
     3481 gaaaccatca cctttgacga cttcgcaaaa gttgacctgc gcgtggcgct gatcgaaaac
     3541 gcagagtttg ttgaaggttc tgacaaactg ctgcgcctga cactggatct cggcggtgaa
     3601 aaacgcaatg tcttctccgg tattcgttct gcctacccgg atccgcaggc actgatcggg
     3661 cgtcacacca ttatggtggc caacctggca ccgcgtaaaa tgcgctttgg tatctccgaa
     3721 ggcatggtaa tggctgctgg tcctggcggg aaagatattt tcctgctaag cccggatgcc
     3781 ggtgctaaac cgggtcatca ggtgaaataa tcccccttca aggcgctgca tcgacagcgc
     3841 cttttcttta taaattccta aagttgtttt cttgcgattt tgtctctctc taacccgcat
     3901 aaatactggt agcatcagca ttcaactgga taaaattaca gggatgcaga atgaaacact
     3961 ttatctatca ggacgaaaaa tcacataaat tctgggcagt tgagcaacag ggaaacgagt
     4021 tgcatatcag ttggggaaaa gttggcacca aagggcaaag ccagataaaa agtttttcag
     4081 atgctgcggc agcggcaaaa gcggagctta agctgattgc ggagaaggtg aagaaggggt
     4141 atgtggagca agcgaaggat aattctttgc aaccttccca aacggtaacg ggctctctca
     4201 aggtagcgga cttatccacc attattcagg aacaaccctc tttcgtagca gaaacccgtg
     4261 cgcctgacaa aaatacagat gctgttttac cgtggctggc gaaagatatt gctgtcgttt
     4321 ttccgcccga agttgtacac accacgttaa gtcatcgccg ctttcccgga gttcctgttc
     4381 agcaagcaga caaattgacc caattacgtc gcttagcctg tagtgtgtcg caacgggata
     4441 ataaaacagc cacatttgac ttcagcgcct gttctttaga atggcaaaac accgtcgccc
     4501 aggcgatcag tcagatcgac ggcctgaaaa caacacagct accatcacca gtaatggctg
     4561 tactcacggc acttgaaatg aaatgcacaa gatataaagt gcgtgaggat gttatggatc
     4621 agatcgtcca ggaaggcggt ctggaatatg ctactgatgt aataatacac cttcaacaga
     4681 ttgatattaa atgggattat gcgaataatg tcattattat tctgccgtct ggcattgcac
     4741 ctgactactt ggagcaatat tccagatttg aattacgcct acgtaaacat ttatcactgg
     4801 cggaagagtc tctctggcaa aaatgtgcac aaaaacttat tgccgcaatt ccacatattc
     4861 cagaatgacg gcaaccatta attgctttgt tattacctga aaaaccagaa attgcacatg
     4921 aaattgccca gcgtctactg gggcaaaaaa aattaccctc gcttgagtgg ttaaaaatag
     4981 tggcgactga tgagcacatt cttgcctcat tagaaaaata tcacgagcca tatgccattt
     5041 ttgatgatta ctattgtggt gcgatatggt cagccaccgt attacaggag caaggtgttg
     5101 cagccctgcc ccgatttgct ccctatgccg caagtgacta ctgcgccgat gtgttgcgtc
     5161 atatcaatca tccgttcgca ttaacactgc ttatacgtgt agccgggcat actaaacgct
     5221 gtcacgatcg gatgacgaaa gccattgctg cgttcccaca tgcagcaatg gcggcactga
     5281 cggaacttct tgggcaaaaa gaagagaaca gttggcgcat tatgctaatg acaatgctta
     5341 tctcacaacc agcactggca gaacaggtca ttccctggct ctcgacaccc gcagttgccg
     5401 tactgaaatc atgccagcaa caactgacac agccctcaaa ccatgccagc gccgatctac
     5461 tgccagccgt agtagtctcc cctccctggc tttcgaaaaa gaaaaaatcg ccgattccgg
     5521 tgctggattt agcgccatta ggcattgagc caatctgtta tctgacagaa gaaatcagta
     5581 atcaactttt ggcgaaatat atctggtatt caaaacacat cacggttagc catgaagaaa
     5641 gtactaccaa cctgttggca aggatgggtt ttcaacgacg gatcgctggt acatatatta
     5701 aagctcccga agcggtagtt gaggcatggc taaatgaaga ttattcaacc ttactaagtg
     5761 aatttaaggt gtttcattca cctaccgggc attattggca gttggggatt ttgacaacat
     5821 tgccgctgga gaaagcagta aaagcatgga atgcccttac cctatctcca cataccgata
     5881 ccgaatacgc catgttacat tttggactca aagggttacc tgggttagta aactcacttg
     5941 cacgctatcc acaagaagcc ttgcccatca cgaattactt cgcagcgagt gagctggctc
     6001 ctgccgtcgc ccgtgccttc aacaaactga aaacgctacg cgaaaacgcc cgtagctggc
     6061 tgttgaaata cccggaacat gcccttaccg gcctgctgcc tgcggcgctc ggcaaagccg
     6121 gtgaagcaca ggataacgcc cgcgctgcct tgcgtatgct taccgaaaac ggtcatcagc
     6181 cattactgca agaaatcgcc cgacgttata accagccgga agtaaccgat gcggtgaacg
     6241 ctctgcttgc gctcgatccc ttagataatc acccgacaaa aatccccact cttccggcct
     6301 tttatcagcc atcgctctgg acgcgcccgg tattaaaagc aaatgcccaa tcactgccag
     6361 atagcgccct cctccacctc ggtgaaatgc tccgcttccc tcaggaagag gctctgtatc
     6421 cgggattatt gcaggtgaaa gacgtctgtt ccgccgactc actggcggga tttgcctggg
     6481 atctgtttac cgcctggcag accgctggcg cgccgtcgaa agagagttgg gcgttcactg
     6541 cgttaggcgt tctcggtaac gatgacaccg cccgcaaact gacgccatta atacgcgcct
     6601 ggcctggtga atcccagcat aaacgcgcca ccgttgggtt ggatattctc gctgctatcg
     6661 gtagtgatat cgcccttatg cagcttaacg gcatcgccca gaaactgaaa ttcaaagcat
     6721 tacaggagcg ggcaaaagaa aaaattgccg acattgccga gagccgcgaa ctcacggtgg
     6781 cggagcttga agatcggtta gcaccggatc tcggtctgga tgataacggt tcgctgctgc
     6841 tggattttgg cccacggcag ttcaccgtca gctttgatga aaccttaaaa ccgtttgtgc
     6901 gtgatgtttc cggcagccgc ctgaaagacc tgcccaaacc gaacaaaagc gatgatgaat
     6961 cgcggtcgaa cgatgcggtt aaccgctaca aattgctgaa aaaagatgcg cgtaccgtcg
     7021 ccgcccagca ggtagcaagg ctggaatccg ccatgtgcct gcgccgccgc tggtcgccgg
     7081 aaaacttcca gctcttcctg gttgagcatc cgctggttcg ccacttaacc cgccgtctga
     7141 tttggggcgt ttatagcgcc gaaaaccagc tactggcttg ctttcgcgta gcagaagata
     7201 acagctacag caccgctgac gatgatcttt tcaccctgcc ggaaggcgat atctctatcg
     7261 gcattcctca cgttctggag atatcaccga cggatgccgc cgcctttggg cagctttttg
     7321 ccgactatga actgttaccg ccgttccgcc agctcgaccg taacagttac gccctgacag
     7381 aaaccgagcg caatgccagc gaattgaccc gctgggcagg cagaaaatgc ccgagcggtc
     7441 gggtcatggg actggcgaat aaaggttggg tgcgtggtga accgcaggat ggtggctgga
     7501 tcggctggat gatcaaacct ctgggccgct ggtcgttaat catggaaatc gatgaaggct
     7561 ttgcagttgg tatgtcgcca gccgaactta gcgccgagca actcttaagc aagctgtggc
     7621 tatgggaagg caaagcagaa agctatggct gggggagtaa ttcaacacag gaagcgcagt
     7681 tctccgtact cgatgccatc accgccagcg agctaattaa cgatattgaa gccctgtttg
     7741 aataaggaaa gagcatggac aaggaattac cgtggctggc ggataacgcc caactggaac
     7801 tgaaatataa aaaaggcaaa acgccgctca gtcatcgtcg ctggccgggc gaaccagtgt
     7861 ccgttatcac tggaagtctc atccagacat tgggagatga attactacaa caagcaggcc
     7921 agaaggaaaa tatcacctgg aattatgata aatgctcact ggagtggcag tccgccatcc
     7981 agcaggcaat caatttgact ggcgagcata aaccgtcaat cccagccctt accatggcgg
     8041 ctctgatctg tatcgcgcaa aatgacagcc agcagttact tgatgaaatc gtgcaacaag
     8101 aggggctgga atatgcgacg gatgtagtga tagcgcgtca gtgcattgcg cgacgttatg
     8161 agagtgactc actggtcgtc actttgcagt atcaggacga ggactatggc tacggttatg
     8221 gctccgcgac ctataacgat ttcgatctcc gtttgcgtaa gcatctgtcg ctggcagagg
     8281 aaagttgctg gcagcgttgt gccgataaac tcattgccgc actgccagga atacccaaaa
     8341 ttcgccgtcc ttttattgcg ctgatcctcc cggaaaaacc tgaaatagcc aatgaactcg
     8401 ccagtcttga aagctctcgg tcaagtctcc attcaaagga gtggttaaag gtcgttgcta
     8461 ctgataatac ggcggtaaaa aaactcgaac gatactgggg cctggacgta tttagcgatc
     8521 gcgaagccag ctacatgtca caagaaaacc gcttcggcta cgcggcctgc gcctcccttt
     8581 tgcgcgaaca aggactggca gccgttccgc gcctggcaat gtatgcccat aaagaagatt
     8641 gcggcagtct gctggtacaa attaaccatc cgcaagtcat ccgcaccttg ctactggtgg
     8701 ctgataaaaa caaacccagc ctgcaacgtg tggctaaata cagtaaaaac ttcccccacg
     8761 cgacgctcgc cgcactggca gaactgctgg cgttaaaaga accaccagcc cgcccaggtt
     8821 atccaatcat cgaagacaaa aagctgcctg cacagcaaaa agcacgcgat gaatactggc
     8881 gtacgctgtt acaaacgctg atggcatcgc agccacaact ggcagcagaa gtgatgccgt
     8941 ggttaagtac tcaagccagg gcagtgctga atagttattt atcggcaccg ccaaaaccgg
     9001 ttattgatag caccgataac agcagtctgc ctgaaatgct cgtttcacca ccgtggcgta
     9061 gtaagaaaaa aatgacagct ccacgtcttg atttggcacc gctcgaatta actccgcaaa
     9121 tttactggca accaggcgaa caagagaggc ttgccgccac tgagtctgcc cgttatttca
     9181 gcacggaatc tcttgcggaa cgcatggaac aaaaaagtgg acgagttgta ttacaggaac
     9241 tgggttttgg ggatgatgta tggctgtttc tgaattatat actccccgga aaactggatg
     9301 ctgcacgcaa ttcactcatt gttcagtggc attactacca ggggcgggtt gaagagatcc
     9361 tgaatggctg gaactccccg caagcacaat tagcagaaca ggcgctccgc agcggtcaca
     9421 tagaagcgtt aattaacata tgggaaaatg acaacttctc acgttatcgt ccggaaaaga
     9481 gtgtctggaa cctgtattta ttggcacagt tgccgcgtga gatggctttg accttctggc
     9541 tgcgtatcat tgagaaaaag catctgttcg cgggtgagga ctattttctc agtatcctcg
     9601 gattggatgc gctaccaggt ctgctgttgg ctttttcaca tcgtccaaaa gaaacatttc
     9661 cgttaatttt aaattttggc gcaacagaac tggcgctgcc tgttgcccgc gtctggcgac
     9721 gtttcgcggc gcagcgtgat ctggctcgcc agtggatttt acaatggccg gaacatacgg
     9781 ctagtgcact tatccctctt gtctttacca aacccagcga taatagcgaa gccgcattac
     9841 ttgccctgcg tttactgtac gaacagggac atggcgaatt gctacaaacc gtggcaaacc
     9901 gctggcagcg tacagatgta tggtctgccc tggagcagtt gcttaaacag ggtccaatgg
     9961 acatttaccc ggcacgcatt ccaaaagccc ctgatttctg gcatccggca atgtggtcca
    10021 ggcctcgact tatcactaat aatcagcctg ttaccggtga cgctctggaa attatcggcg
    10081 aaatgctgcg ctttacccag gggggacgtt tttatagcgg gctggaacaa ctgaaaacgt
    10141 tctgccagcc acaaacgctg gcagcttttg cctgggatct cttcactgcg tggcaacaag
    10201 ctggtgcccc cgcaaaagac aactgggcat ttctggcgtt aagtctcttt ggtgacgaaa
    10261 gcacggcacg ggatctgacg acacagatcc tcgcctggcc acaagaaggc aaatctgccc
    10321 gtgctgtcag cggcctgaac atccttaccc tgatgaataa tgatatggcg ctgatacagc
    10381 tgcatcatat atcgcaacgg gctaaatccc gccccttacg tgataacgcg gcggaatttc
    10441 ttcaggtagt cgcagaaaat cgcgggctaa gccaggaaga gctagcggac agattagtcc
    10501 caaccctggg ccttgatgat ccgcaggcgt tgagttttga ttttggtccc cggcagttta
    10561 ccgttcgctt cgatgaaaac ctcaacccgg ttatctttaa tcagcaaaac gttcgccaga
    10621 aaagcgttcc ccggttgcgc gccgatgacg atcaactgaa agcgcccgag gcactggccc
    10681 gactaaaagg gctaaaaaaa gatgctactc aggtgagcaa aaacctgctc ccgcgtcttg
    10741 aagctgccct acgtaccacc cgacgctggt cgctggcaga ttttcattct ctgtttgtta
    10801 atcatccctt tacccgtctg gttacccagc gattaatatg gggggtttat ccggcaaatg
    10861 aaccgcgttg tttactcaaa gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
    10921 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
    10981 tgacagtaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
    11041 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
    11101 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca gcttatggga atgcgataca
    11161 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
    11221 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
    11281 tccgttctct tcgagtgcac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
    11341 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaaa cacagcgggc
    11401 agtgatgcaa ctgcccgtta tcaacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
    11461 aaagagctgg ttgctattta cgattatgag atccctgtac ctgaaaatcc gttttccttc
    11521 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctatcg actggagcga
    11581 ttccggctac gtccaacatt tcatcaacgt gatcgagaag atactgaccc gctaataaat
    11641 gatgcgttga tttatataag agatgagtgt attgatgagc ggaaattacg aggtgaatca
    11701 cctgaaactg taatagcaat ttttaatcgt gaactacaga atatattcaa ccaagaaata
    11761 gaataatata ctctagataa ttaaagtcgc agcacatgca acttgaagta tgacgagtat
    11821 agctttctta aagggggaaa cggtattccc ccttagatgc gtttcaattt gcccgttact
    11881 tcaacaaatc aatcaaccgt aacagagcca ctaataactc cagtacggca atgagcacgg
    11941 ttaacttttg tgtcagcgtg tccatccgct ctccttatta aggagcgcaa caccagcacc
    12001 ttacccttac actgcctgtt gatactggtt tacgtatgtt ggtacgaaac gttgctctcc
    12061 ggatcagggc accgcctgcg gcaccacccg tccggcagcc aggacttaaa attggtccga
    12121 tccggcacaa cctcccaacg ggcaggatta taaatctcac tcactgaatt gcaatggagt
    12181 gtggatatgg ttataattta ttcgttggta cgaaatgttg cacaatcagc aagttagcgt
    12241 cactgtagta aataaaagaa cctgcctcac cagcaggttt ttttattttc tgcgatctac
    12301 tttccagata ttttttgcca atagcccaac ataaatattc acatcccggt ca
//
LOCUS       DAZFRJ010000134        12305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000134 DAZFRJ010000000
VERSION     DAZFRJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.135"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..729
                     /gene="ybjI"
                     /locus_tag="ACHKUN_003367"
     CDS             <1..729
                     /gene="ybjI"
                     /locus_tag="ACHKUN_003367"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO6661573.1"
                     /translation="YQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVV
                     SEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYH
                     RLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGNGSIDLIIPG
                     VHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRA
                     GSNNREGVLDVIDKVLKHEAPFDQ"
     gene            815..1099
                     /gene="ybjH"
                     /locus_tag="ACHKUN_003368"
     CDS             815..1099
                     /gene="ybjH"
                     /locus_tag="ACHKUN_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO6661574.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHKUN_003369"
     CDS             complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHKUN_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO6661575.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHKUN_003370"
     CDS             2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHKUN_003370"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6661576.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            3311..4069
                     /gene="deoR"
                     /locus_tag="ACHKUN_003371"
     CDS             3311..4069
                     /gene="deoR"
                     /locus_tag="ACHKUN_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO6661577.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHKUN_003372"
     CDS             complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHKUN_003372"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6661578.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            5565..6191
                     /gene="gstB"
                     /locus_tag="ACHKUN_003373"
     CDS             5565..6191
                     /gene="gstB"
                     /locus_tag="ACHKUN_003373"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO6661579.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHKUN_003374"
     CDS             complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHKUN_003374"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO6661580.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHKUN_003375"
     CDS             complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHKUN_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO6661581.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            8010..9335
                     /gene="rimO"
                     /locus_tag="ACHKUN_003376"
     CDS             8010..9335
                     /gene="rimO"
                     /locus_tag="ACHKUN_003376"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO6661582.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(9382..10710)
                     /locus_tag="ACHKUN_003377"
     CDS             complement(9382..10710)
                     /locus_tag="ACHKUN_003377"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6661583.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(10718..>12305)
                     /locus_tag="ACHKUN_003378"
     CDS             complement(10718..>12305)
                     /locus_tag="ACHKUN_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO6661584.1"
                     /translation="YIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVL
                     LVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEM
                     KDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVD
                     ERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGY
                     RNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWI
                     ARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIG
                     FINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENG
                     LKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDS
                     IKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGY
                     LYQKPMPFSAWDKSGKLVKE"
BASE COUNT         3214 a         3119 c         2864 g         3108 t
ORIGIN      
        1 tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat
       61 cagttgatct ctttcttccc tgaaattgcg aatgaaattg cctttgtggc tgaaaacggc
      121 ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt
      181 gctactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa
      241 aatagcgcct atacactcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
      301 tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatttt ctttaagttt
      361 ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc
      421 ggcgatatta tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc
      481 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
      541 gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt
      601 tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg cgcaggctcc
      661 aataaccgtg aaggcgtact ggatgtgatc gataaagttc ttaaacacga agcgccattt
      721 gaccaataaa aacggcagca cgcccggcaa cacaatgcct gaatgaaggc tgcataattc
      781 gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt
      841 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
      901 gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag
      961 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
     1021 gcatccttat gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
     1081 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt
     1141 tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
     1201 cacaaaattc cgttgacaag gttgaagaga ttaaacagtc cgttgccccc gttcagccag
     1261 gcatgtttgc tgatttcaat accgacggta aagatcagca tttgcagcat tcccatcgcc
     1321 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
     1381 ttcgccagac caatgccgaa agcataaata cttaacccgg cggtcatcca taaatacgcg
     1441 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
     1501 cccataatta tcagcgaacg tacggtgcgg cgcgaggtca aacgcgctaa caacaagtta
     1561 cccgcaatta acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac
     1621 tgctcgccgg taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta
     1681 acgaatccca gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag
     1741 tcacgaccga gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg
     1801 gcttgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaagac aaacatccct
     1861 tcccagggca gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc
     1921 agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc
     1981 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc
     2041 aacagagtga attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac
     2101 cacaccaccc ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg
     2161 agccattgta aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca
     2221 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg
     2281 atataggttg aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca
     2341 agcctggcac cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc
     2401 agagtttaat aaaagcacag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa
     2461 gcacattctt acattattgc gagtatagct acgctttctt taagttttat ttaacctatg
     2521 cccgttacaa ttacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta
     2581 cccagtttaa cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac
     2641 tttgcgtcgg gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc
     2701 gacgccagac tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat
     2761 taccgtggta ccgctgctgg ccgcggtgct ttggttgtgg gggcttacag cacaacggca
     2821 actggtgata aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg
     2881 acatcttttt ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca
     2941 tgcggcggat gactcattcc caagcgacca cggtacggtg attttcacct ttgcactggc
     3001 atttttatgc tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat
     3061 tgcctggtcg cgcgtttatc ttggcgtgca ctggccgctg gatatgctcg gtggattgct
     3121 ggctggtatg attggctgcc ttagcgctca gattatctgg caagcgatgg ggcataaact
     3181 ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg
     3241 ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt
     3301 cagagggatt atggaaacac gtcgcgaaga gcgcatcggg cagctgctgc aagaattaaa
     3361 acgcagcgat aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac
     3421 gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt
     3481 tctggaaccg cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga
     3541 agaaaaacgc cgggcggcaa aaatggctgc gacactggta gaacccgatc agactctctt
     3601 ttttgactgt ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt
     3661 taccgccgtt tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg
     3721 cgcatttctg tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt
     3781 tcagcaaacg ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca
     3841 tgtcagtaaa ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat
     3901 gtcgatggcg caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc
     3961 ggcgcgcatg ggtgacctga aacgctttga tattgtggtg agtgattgtt gcccggaaga
     4021 tgagtatgtg aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac
     4081 cggatgacgt ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac
     4141 tgatggaatt tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca
     4201 ttttccatca cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg
     4261 gtcccgacaa cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag
     4321 ctcgccttca ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc
     4381 ccaagattca cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca
     4441 ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac
     4501 tcattaaaac ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg
     4561 ccctgggtag ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg
     4621 ccatcaacat tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg
     4681 ttgaaggtga actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat
     4741 gctttaccca gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg
     4801 tgcaccgtct ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa
     4861 ccaatgaatg actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta
     4921 ccggactgga taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg
     4981 aggaacatta ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg
     5041 accgtcacca tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag
     5101 ctggtcatga ttttagtcag gctcgcggga tccagtttct catccgcgtt gccttctgcc
     5161 agcactttac cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct
     5221 tcaacggttt gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc
     5281 gctgcaagac cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt
     5341 caaaaaaaat acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa
     5401 cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc
     5461 tgcttctggt gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg
     5521 gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg
     5581 gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac
     5641 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
     5701 cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt
     5761 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgtctgtgga
     5821 tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca aaccagacgc
     5881 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
     5941 atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg
     6001 cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta
     6061 tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc
     6121 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
     6181 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
     6241 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
     6301 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
     6361 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga
     6421 aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
     6481 cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg
     6541 ccagcggcca gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg
     6601 ggccatgttc attcagccac agtgcattac tccacggatt cattgccatt ccttgcggat
     6661 tacgaatgcc ataagaccag atctcggcgc gcacaccgga ttcctttata aaaggattat
     6721 catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat
     6781 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
     6841 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcggaaga
     6901 cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
     6961 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag
     7021 caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg
     7081 gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
     7141 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt
     7201 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag
     7261 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
     7321 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
     7381 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
     7441 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
     7501 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
     7561 taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa
     7621 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
     7681 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc
     7741 aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact
     7801 tcgatcctcc tctttccagc cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa
     7861 tgctgtccag gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg
     7921 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
     7981 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
     8041 gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac
     8101 tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca
     8161 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
     8221 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
     8281 gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg
     8341 agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag
     8401 aacaaggtgt gaaactgacg ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta
     8461 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga
     8521 ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg
     8581 tgatctcgca ggatacttct gcctatggcg ttgatgttaa acatcgtact ggcttccaca
     8641 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
     8701 tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga
     8761 tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
     8821 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
     8881 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg
     8941 agacagaaga agatttccag atgctactcg acttcctgaa agaagcgcgt ctggatcgcg
     9001 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
     9061 ttccggaaga agttaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt
     9121 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
     9181 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
     9241 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg
     9301 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
     9361 tgtccggcct gcgaataatg attactgcca gctaatctga gccctgccgt ttttcttact
     9421 cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac attcatttga
     9481 atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat gaacgatttc
     9541 ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg caaattcgtc
     9601 accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa ggattttcac
     9661 cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa
     9721 tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc
     9781 gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat
     9841 actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc
     9901 aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca ctctgcgtac
     9961 cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa
    10021 tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa
    10081 ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cataatattc
    10141 atcaccggtc accggtttac cgttcttaaa tagagagaac atcaggttct cgctgtccat
    10201 cgccagcgat tttgacagta aagaaaaacg ctccagacca agcaaaaata tcagactacg
    10261 attgtgagtt accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc
    10321 tgtatttttg acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc
    10381 gaactccttt acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa
    10441 ataagagggg gaaaccgagt cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc
    10501 cgtgggattt agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt
    10561 tgccaccgca aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac
    10621 catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac
    10681 tgtaagtacg aacttattga ttctggacat acgtaaacta ctcttttact aattttccac
    10741 ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac
    10801 atcctaattt aattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg
    10861 ggatccggtt atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga
    10921 aaatatcgcc ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac
    10981 gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac
    11041 gcagttgttc aatattgaga taaaaatgtt cattaattcg ctcgatcacg gtttcagtga
    11101 tctcaacaca gaggctgttc gccttcaggc cattttcttt catgatggtg gtaaaacgct
    11161 catgaaagtg cggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat
    11221 ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct
    11281 gataacccag ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg
    11341 aaatgatccc ccgctccgca gaaacccaac ggcacaacgc ctccgcgccc agaatggcgc
    11401 gggtgttaat atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat
    11461 ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac
    11521 agaaaataaa ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt
    11581 ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc
    11641 gcagattgcg gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa
    11701 atgtctgtac attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat
    11761 cgcagcattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
    11821 gttcatcaac accaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca
    11881 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt
    11941 cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca
    12001 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca
    12061 gagcctcgat ctgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca
    12121 tttcagaaaa ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga
    12181 taataatgaa cgcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc
    12241 caggataata aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa
    12301 tatac
//
LOCUS       DAZFRJ010000135        12146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000135 DAZFRJ010000000
VERSION     DAZFRJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.136"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(48..950)
                     /gene="yihR"
                     /locus_tag="ACHKUN_003379"
     CDS             complement(48..950)
                     /gene="yihR"
                     /locus_tag="ACHKUN_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO6661585.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1088..2329)
                     /gene="yihS"
                     /locus_tag="ACHKUN_003380"
     CDS             complement(1088..2329)
                     /gene="yihS"
                     /locus_tag="ACHKUN_003380"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO6661586.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2346..3224)
                     /gene="yihT"
                     /locus_tag="ACHKUN_003381"
     CDS             complement(2346..3224)
                     /gene="yihT"
                     /locus_tag="ACHKUN_003381"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO6661587.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3248..4144)
                     /gene="yihU"
                     /locus_tag="ACHKUN_003382"
     CDS             complement(3248..4144)
                     /gene="yihU"
                     /locus_tag="ACHKUN_003382"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO6661588.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4312..5208
                     /gene="yihV"
                     /locus_tag="ACHKUN_003383"
     CDS             4312..5208
                     /gene="yihV"
                     /locus_tag="ACHKUN_003383"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO6661589.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5242..6027
                     /gene="csqR"
                     /locus_tag="ACHKUN_003384"
     CDS             5242..6027
                     /gene="csqR"
                     /locus_tag="ACHKUN_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6661590.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6126..6725
                     /gene="yihX"
                     /locus_tag="ACHKUN_003385"
     CDS             6126..6725
                     /gene="yihX"
                     /locus_tag="ACHKUN_003385"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO6661591.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6719..7591
                     /gene="yihY"
                     /locus_tag="ACHKUN_003386"
     CDS             6719..7591
                     /gene="yihY"
                     /locus_tag="ACHKUN_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO6661592.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7588..8025
                     /gene="dtd"
                     /locus_tag="ACHKUN_003387"
     CDS             7588..8025
                     /gene="dtd"
                     /locus_tag="ACHKUN_003387"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO6661593.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8070..9011
                     /gene="fabY"
                     /locus_tag="ACHKUN_003388"
                     /gene_synonym="yiiD"
     CDS             8070..9011
                     /gene="fabY"
                     /locus_tag="ACHKUN_003388"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO6661594.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9075..9983)
                     /locus_tag="ACHKUN_003389"
     CDS             complement(9075..9983)
                     /locus_tag="ACHKUN_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6661595.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            10212..10523
                     /locus_tag="ACHKUN_003390"
     CDS             10212..10523
                     /locus_tag="ACHKUN_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6661596.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            10524..10814
                     /gene="nadS"
                     /locus_tag="ACHKUN_003391"
     CDS             10524..10814
                     /gene="nadS"
                     /locus_tag="ACHKUN_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6661597.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            11419..11637
                     /gene="yiiE"
                     /locus_tag="ACHKUN_003392"
     CDS             11419..11637
                     /gene="yiiE"
                     /locus_tag="ACHKUN_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6661598.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            11856..12098
                     /gene="yiiF"
                     /locus_tag="ACHKUN_003393"
     CDS             11856..12098
                     /gene="yiiF"
                     /locus_tag="ACHKUN_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO6661599.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT         2980 a         2938 c         3202 g         3026 t
ORIGIN      
        1 agcgagtgaa atgttcttca atgattttga ctgctaatca atacaagtta ttttctctgt
       61 ccgtaaatgt taaaaaacag gcggtgcggt tttcctggtt ccagtaataa taagtcgata
      121 cctgaattaa aggcgttagg cgggcaactc ataggttcaa ctgccagtcc ctgacgctgt
      181 aatttttcac cgctataaat ttgtacccag ggttgatcgc aacaaaggct aaccgaaagc
      241 gcctgttgtg gatgggtaat ggttatttcc cacagatcat tagcggtttt aaaggtgtgg
      301 tcgatttttg tcgcggctat tgttttcgcc tgcgtgaaat ttaaatccag ctcatcaacg
      361 tggtgaagcg ttgtcggatt tacgtgttca tcaacggcat aaacctggtt tgccggaagc
      421 tggaacagat attcatcaac agatgttaag ttgcaggtta aataagtatg aatacccacg
      481 ccataaggcg ctgcgacagt gccgatattc tggcttgcga tctcaacgga aagacccgtg
      541 tgtgcgttga gcgaataaac aacttgtgaa gccagcataa acggatagcc gtaactgggc
      601 ggtagaaaag ctgtcagggt aacgctggtg gcggttaatt cactaatttg ccagtctcgc
      661 caggcaagca gcccgtgaat agcggctttt gaactatgtt cattaatggg taactgatat
      721 tcctgcccct gatagcggta acagccgttg gcgatccggt tcggccacgg aataagcact
      781 ttacccagat gcgccagcgg catctcttcg ggtttgtgcg ggatgactaa atgacaaccc
      841 tgaaaggtta attcagccag accagcaccg acggtcacaa tggtcgcgtg ataatccccc
      901 gcggcaagtg aaacggtctg tccactgcaa tgcatattgg tgatttgcat acaaggcacc
      961 ttaattaacg acatacagcc tcccgtgagg ctgatgtcaa acccaaaaac tgcaccggac
     1021 ggtcccctcg cccctttggg gagagggtta gggtgagggg aaaacccgca ccctattttc
     1081 agatcagtta tttcgcatta atatccagca gacccgctgc aaccgctgga gccatgccgg
     1141 gcgctaacgg gatacgcggg atcaccaggc aatgcagcag gtgataaata tcctgtttgc
     1201 cgtcccagac tttggtggtg accttattgt ccgcatccag ctcctgccac caggaaccat
     1261 tttcatagtc catcaggtac ttaatgcagt actcccacca tgtttgatac caggtttcat
     1321 actggcgatc gccagtgacg gtgtagagcg cgtaggccgt acccattgct tcgacgatag
     1381 gccaacgtac acgttcgcgg accaccggtt ttccttccca gtcaacggta taaacaatcc
     1441 cgtccgcgcc atcgggtgcc caggcatcgc gcacggtggc gttaaacaga cctttggcat
     1501 cttctagcag ccatgctggt ggttgttcgc aacgggcttc cagggcagca tggatgtgca
     1561 gcattaaacg gccccattcg atccagtggc ctggtgtacc tccgaacgcg cggaagcgat
     1621 gcgccgggtt atctttgttg taatccggca gcggattcca ctgggtatcg aagtgttcgt
     1681 taacgcgata atgattattt ctggcgacgt cgtggataat cacggaagcc acgcgaatcg
     1741 cgcgatccag ccattttttg tcgtgagtga cgtcataaac aatcaagaaa gcttccaccg
     1801 cgtgcatatt ggcattgccg ccgcggtact cttcggtttt gctgaaggct tcgtcccagg
     1861 attccaggca catctgctct tcttcgctcc agaaatattt ctcgataatt tcaatggtgt
     1921 aatcgagcag cttgcgcgct tccgggtgac ccgttgtgac ggcgctggcg gcacccagca
     1981 gagcaaagaa gtgttgatag ccctgtttgg aggcatccac cacgccctcg tcatttacgc
     2041 aggcatacca gccgccatat tttttatcac gcagtgcgcc gttcatggct ttgataccgt
     2101 gatcaaccaa cgagtaagcg ccaggtcgac ccatcgccgc agcaacggaa taaacgtgta
     2161 acatacgagc ggtgatccac agatgggtgc ccatctcttc tttgatttgc cctttattgc
     2221 ctaaccagcc aaaaccagtc ggcactactg aatttttacc aaaatcaaag atgcggtcgg
     2281 tttcctgttc cagccaacgg ttgtggctta gggtgttaaa ccatttcatt ccttttatcc
     2341 tcatcttagc ggcgtttagc catcatttcg tcgacgatat cgccaagttg ttgtaatttc
     2401 ggtgcgcaaa cgtcacgcag catcagctcg ttgtctggta aaccgacgac cgatgcccag
     2461 actgcacggc ctgccaggaa tcccgatgcg cccgccgtca ttgccacgcg tacggcacgc
     2521 gggaacagtt tttcgtcgac accggaagag aggatcaccc atggcatatt gatatggtca
     2581 ttcagacgtt gtgaagcaca gagaagctct tgttgcggac ctttgccata aagcggcatt
     2641 tcaactttgt agaggtcagc gccactgtcg cccagctctt tggcggcatc gatgatcgct
     2701 tgttcgcgat cgaatttatc gccacgacgc ggtgggcgga cgactggctc aatgatgctt
     2761 accagaccgt gtgagtggca cagttcgttg aactctttca ccatatccag acgttgctgc
     2821 gcatcttcat cactacgcca cagcaccagc agttttaagg ctttgccacc gtcttgtttg
     2881 atctgtagcg gattgatttt gcggtcaata accacgctat cgaccggaat accgttgcca
     2941 ggaatgaact catcggcggc gacaatcatg gcgcaacttt tggcaatcgc gttttgctca
     3001 accacctggc ggtagcagaa ttgttgatct accagaatcg ccgaggcata aggcgagagg
     3061 gcctttgcag cgttaacttt gaaatcagtt aaaacgctat cggctaccgg agcgggtgcc
     3121 ccagccgcgg caaacatcat gcgcatggct tcgcgctgat cgaccgccag catggcaaaa
     3181 ccgcccgatg cgcgcgtaat gtcgttgatg gtgtacttat tcattcattt attcctttat
     3241 ccagtcgtta catttttact ttggcagtca tcccggcact gacacggacc tgttccagaa
     3301 tggcggacca gtcctggcga ccgcgacccg ctgcgcgcgc ctggctataa acctcccgtg
     3361 aggcggcccc cagcggcatt ggcacatgca gctggttggc gacatcaagg gcgatgccaa
     3421 gatccttatg ggcaagatcg atcatgaagg cgggagaaag atctccgctg aggactttgt
     3481 ttggccagga agtggtgaag tgacctttac cggcggcggt accgctcatc actttgacgg
     3541 caacatcgaa gggaagattc agggcttcgc acaaaacggc ggcttctgcc gaaagcgcat
     3601 tgagcgcgat gctcatgtag ttgttgatga gcttaacgcg gatccccatg cccggaccgc
     3661 cagcgttgat caactcactg cccatcgcca tcaggatcgg tgtggcacgt tcaacttgtt
     3721 cagcggtgcc gccagccagc agtaacagag taccggtaat ggcatttgca gaagtacggc
     3781 ctaccggaac atccatcatg ctgaagcctt tggcttgcat atcggcaatc aatttatcgg
     3841 tttgcagcgg atggatggtg gacatatcaa tgaccagcgc atcggtagat aagccttcgc
     3901 aaacgccgtt ttcaccgaac aacacgttgc gcaccagatc gccattcggc aacatggtaa
     3961 tgataaattc ggcatcttta gcggcctgcg ccgggttggc ggcgggagtc gcacctttgt
     4021 ctaccagatg ccgcacagct tcggcgttca catcaaagac gcgaagttga tgcccttgct
     4081 gcaataaatt gctcgccatt ggcgaaccca tttgtcctaa accgataaac gcgattgctg
     4141 ccataaccct ctcctgaata cagtgatgtc actttttgtc atttatgaca tgctttgctt
     4201 gtctgttttt gatcgtattt gtaatttatc gtcaaaaaat tgacagccgt cactttttaa
     4261 acaattggtg aaattaaaat gaacgcatcc caaaatgttt aaggaatgac catgattcgt
     4321 gttgcttgtg taggtataac cgtgatggat cgcatctatt acgtggaagg gttaccgacg
     4381 gagagcggta aatacgtggc gagaaattat acggaagttg gtggcgggcc agcggcgacg
     4441 gcagcggttg cggcggcaag gctgggggcg caggtcgatt ttattggtcg cgtaggtgat
     4501 gacgacacgg gcaacagcct gctggcagag ctggaatcct ggggcgttaa cacccgttac
     4561 accaaacggt ataaccaggc gaaatcttcg caatccgcca tcatggtgga taccaaaggc
     4621 gagcggataa tcattaacta ccccagcccg gatctgctgc ctgacgcaga gtggttggag
     4681 gaaattgatt tctctcagtg ggatgttgtg ctggcagatg tacgctggca cgacggcgct
     4741 aaaaaagcct tcaccctggc ccgtcaggcg ggtgtgatga ccgttctgga cggggacatt
     4801 acgccgcagg atatcagtga gctggtggca ttaagcgatc acgcggcctt ttcagaaccg
     4861 ggtctggcgc gcttaacggg cgtgaaagag atggccagtg cgctaaaaca ggcacaaacg
     4921 ctcacaaatg gtcatgtcta tgtgacccag ggtagcgcag gctgcgactg gctggaaaat
     4981 ggtgggcgtc agcatcaacc ggccttcaaa gttgatgtgg tagataccac aggtgcgggt
     5041 gatgtttttc acggcgcttt ggcggtggcg ctggcaacaa gtggggattt agcggagtca
     5101 gtccgcttcg ccagcggtgt agcggcgtta aaatgcacac gtcccggtgg acgagccggg
     5161 atccctgact gtgatcaaac ccgatctttt ttgtcacttt ttgtataaaa tgccagggtg
     5221 atggtttttc gaggaatttt catgagcctt accgaactaa ccggtaaccc gcggcacgac
     5281 caactcctca tgctgatcgc cgagcgtggg tatatgaata ttgatgagct ggcaaatctg
     5341 ctggatgtct ccacgcagac ggtccgccgg gatattcgta aattaagcga gcaaggcctg
     5401 attacgcgcc atcacggtgg cgcgggtcgg gcttccagcg tcgttaatac ggcgttcgag
     5461 cagcgtgagg tttcgcaaac cgaggaaaaa aaagcgattg ccgaagcggt ggcagactat
     5521 attcctgatg gttcaacaat atttatcacc attggtacga ctgttgagca tgttgcccgg
     5581 gcgttactta accataatca tttgcggata atcaccaaca gcctgcgtgt ggcgcatatt
     5641 ctttaccaca acccgcgctt tgaagtgatg gtgcccggcg gtacgttgcg ctctcataat
     5701 agcgggatca ttggcccttc agcggcgtcc tttgtggctg attttcgtgc tgattatctg
     5761 gtaacaagcg ttggggcgat tgagagcgat ggcgcgttga tggagtttga tgtaaacgaa
     5821 gctaacgtgg tgaaaacgat gatggcgcac gcgagaaata ttctgctggt cgccgatcac
     5881 actaagtatc atgcttcggc ggcggttgaa attggtaacg tggcacaggt cactgcgctc
     5941 tttaccgacg agctgccgcc cgctgcgcta aaatcacgct tacaagacag ccaaattgaa
     6001 atcattcttc cccaggaaga cgcgtagatt tttgtgaccg ttaacctggc ttcatacctt
     6061 gccacatagc caaacccatc ctttcccgct acagttaatt tcttgtggcg cgaaaggagg
     6121 caaaaatgct ctatatcttt gatttaggta atgtgattgt cgatatcgac tttaaccgtg
     6181 tgctgggagc ctggagcgat ttaacgcgtg ttccactggc tacgcttaag aagagtttcc
     6241 acatggggga ggcgtttcat cagcatgagc gtggggaaat tagcgacgaa gcgttcgcag
     6301 aggcgctgtg tcatgagatg gccctgccgc taagctacga gcagttctct cacggctggc
     6361 aggcggtgtt tgttgcgctg cgcccggaag tgatcgccat catgcataaa ctgcgtgagc
     6421 aggggcatcg cgtggtggtg ctttccaata ctaaccgcct gcataccacc ttctggccgg
     6481 aagaataccc ggaaattcgt gatgctgctg accatatcta tctgtcgcaa gatctgggga
     6541 tgcgcaaacc tgaagcacga atttaccagc atgttttgca ggcggaaggt ttttcaccca
     6601 gcgatacggt ctttttcgac gataacgccg ataatataga aggagccaat cagctgggca
     6661 ttaccagtat tctggtgaaa gataaaacca ccatcccgga ctatttcgcg aaggtgttat
     6721 gctaaaaacc attcaggaca aagccaggca ccgtacccgt ccactatggg cctggctaaa
     6781 actactctgg caacgtattg atgaggacaa catgacaacc ctggcaggta accttgccta
     6841 tgtgtcgttg ctctcattag tgccgctggt tgccgttgtt tttgcgcttt tcgccgcttt
     6901 tcccatgttt tccgacgtca gcattcagtt acgtcacttt atttttgcca actttctgcc
     6961 tgctactggc gatgttattc agcggtatat cgaacaattt gttgccaatt ccaacaagat
     7021 gaccgccgtt ggggcgtgcg ggctgatcgt caccgcgtta ttgctgatgt actccatcga
     7081 tagcgcgttg aataccatct ggcgcagtaa acgagcgcga ccaaaaattt actcgttcgc
     7141 cgtgtactgg atgattttaa cgctggggcc gctgctggca ggggccagtc tggcgatcag
     7201 ttcctatttg ctctccctgc gctgggcgag cgatctcaat actgtcatcg ataacgtgct
     7261 gcgtattttt ccgctgctgt tgtcgtggat ctccttctgg ttgctgtaca gcattgttcc
     7321 taccatccgc gtacctaacc gcgacgcgat tgtcggcgcg tttgtcgccg cgctcctgtt
     7381 cgaagcagga aagaaaggtt tcgcgcttta tatcaccatg ttcccgtcat atcagctcat
     7441 ttacggcgtg ttggcggtga tccccattct ctttgtttgg gtctactgga catggtgtat
     7501 cgtcttgctt ggcgcggaaa ttactgtcac tctcggggaa taccgcaaac taaaacaagc
     7561 agctgaacaa gaagaagacg acgaaccatg attgcattaa ttcaacgcgt aacccgtgcc
     7621 agcgtcaccg tggagggaga agtgacgggc gaaattggcg cgggactttt ggtgttattg
     7681 ggtgtcgaaa aggatgacga cgaacagaaa gcaaaccgtc tgtgcgagcg tgtgctcggc
     7741 taccgcatct ttagcgatgc cgaaggcaag atgaatctca acgtgcaaca ggcgggcggc
     7801 agtgtactgg tggtttccca gtttaccctc gccgcagata ccgaacgggg gatgcgccca
     7861 agtttctcta aaggcgcatc accggatcgc gcagaggcgt tatatgatta tttcgtcgaa
     7921 cgctgccgcc agcaagagat gaacacgcaa acaggacgct tcgctgccga tatgcaggta
     7981 tcgctggtca atgatggccc cgtgacattc tggttgcagg tatgagccag cttccagggt
     8041 tgtcacggga aacaagagag agtatcgcta tgtatcacct tcgggttcca caaacagaag
     8101 aagagttaga gcgttactat cagtttcgct gggaaatgtt gcgtaagccc ctgcatcaac
     8161 caaaaggttc ggaacgcgac gcgtgggatg cgatggcgca tcaccagatg gtcgtcgacg
     8221 agcagggtaa tctggtggcg gtaggccgat tgtatattaa tgccgacaat gaagcgtcca
     8281 ttcgctttat ggccgttcac ccggacgtgc aggacaaagg gttaggaacg ctgatggcga
     8341 tgaccctgga gtcggtggcg cgtcaggaag gcgttaagcg cgtgacctgt agcgcccgtg
     8401 aagacgcggt ggagtttttc gccaagctgg ggtttgttaa tcagggagaa atcaccacgc
     8461 caaccaccac gccgattcgc cattttttga tgattaagcc cgtcgccact ctggatgata
     8521 ttttgcatcg cggcgactgg tgcgcgcagc tgcaacaggc gtggtacgaa catatcccgc
     8581 ttagtgaaaa aatgggcgtg cgtattcaac aatataccgg gcaaaaattt atcaccacca
     8641 tgccggaaac cggtaatcag aatccgcacc atacgctgtt tgccgggagt ttattctcac
     8701 tggcaacgct caccggttgg gggcttatct ggctgatgct gcgcgaacgc catctcggcg
     8761 gaacgattat tctggcggat gcgcatatcc gctacagcaa gccgattagc ggtaaacctc
     8821 atgcggtagc cgaccttggt gccttaagcg gcgatctcga ccgtctggcg cgcggacgaa
     8881 aagcacgggt gcagatgcaa gttgaaatct ttggcgacga gacgccgggt gcagtgtttg
     8941 aaggcacgta tatcgttctg cccgcgaagc catttggccc gtatgaagag ggcgggaacg
     9001 aagaagagta ggggcgtttt acttgactga aaaggttttc aatctgaaaa ccttttcagg
     9061 gcccaggtcc gaagttacct tcccgctctc caggcaacat cccgtagtgc cgatcgcgcg
     9121 gcctcactga taccacctaa ctgaaaaaat ccgtggataa cgccaaggta gcgttggcag
     9181 gtgcattgca ctccttgccc tgtcatgcgg tgatacaacg cttcaccttc atcgcaaagt
     9241 gggtcgtatt cagcggtaat gatatgtaca ggtggcaagc cattgaaatc atcgcgccaa
     9301 agagggctgg cttcaggatg cctgcgttca atgcctggca gatacatctc aaagccgctc
     9361 agtaaagtgt cgcgtgtgat gatgtaatcg tgaccattgc gtatgtagct ttcgaaatgc
     9421 gcagtcgcat caagcatagg ataaattaag atgagttgtg ccggttgcca ctcgccagca
     9481 cgctttaatc gcaaagcagt taccagcgcc agatgtccgc ccgcactgtc accggcaaga
     9541 gttatacgtt gtttatcaac gccgagtcgt tcggcgtgtt gccagactat ttctgcacct
     9601 cgttgcgcat catcatgggc agcggggaaa gtatgttccg gtgccagccg atactggacg
     9661 gcaataaccc gacattgccc gtaataggct aactggcgca gctggttgtc gtgagttgcg
     9721 aacccgccgc taacaaaaca gccaccgtag taataaatta tcgtcgggag taatgtcggg
     9781 gcattcagcg gcgacactat tcgtaaggtc atcccttcaa gttcgatagt ctgaatatct
     9841 acccgcgttt ctgtctcacc cgcaaggaca gcgctggcga tatagccctc cctgcgctgc
     9901 gcaatacttt gttgacgcga tgaggggcga cccgcagcga taaacttttc gactaactct
     9961 gcaattcctt tttccagtgc catagtagaa cttattttgc tgtatgaatg tacagtctta
    10021 tacacccgtt tttgctggat ggatacaggg aaattcacgg gcgtgaaaaa tctggaaagc
    10081 gcctcgcaaa tctaaagaca aacccggcag agacgtggtt ttgctggagc actatcagta
    10141 agctagtggt ttcaagggta cgtcaatgac gtacaaaaag gattgttact ctaagccaat
    10201 ggcgtataat tatgctcttc atcgaaacgg aaatttttac tgaagatgtt caaaaactgc
    10261 tgaccgacga tgaattcagt cgttttcagt ttttcctggc cttgaatcct gactatggtg
    10321 aggtaatacc tgaaactggt gggttaagaa aggttcgatg ggtgtcagga ggaaagggga
    10381 aacgtgctgg tgtcagggtt atttactttc atcaggttaa acattatgag atcagattac
    10441 tgcttattta tcgtaagggt attaaagatg atctgtctcc ccaggaaaaa gcaatgctcc
    10501 ggttgttaaa tacgaggtgg taaatggata aggcactttt tgagcggttg actcacagca
    10561 tggcccagat gaatgaaatt attgaaggaa cgcgacagcc ttcgcggact tttgaagttg
    10621 atgctatgaa aatcaaagaa atacgccgcg cttcagggtt gtcacaatct aaatttgccg
    10681 atcttatttc tgtaagcgta gacacgttgc gtaactggga gcaggggcga cgttcaccga
    10741 ccgggcctgc aaaggctttg ctgcgagcga ttgccaatga tccgcaacat gtgctgcaag
    10801 cacttaaccg ttagaggcat ggttatgcca tacagaaagc actagcattc agaactcctg
    10861 tgaaagcggc atagtggtcg cctgcccagg agttttgcta tgacccgtaa tcgtgctgaa
    10921 cgtcgccacc ctatgatgcg attaaaaaaa ctcatcgtcg cgctgatgat tgacgtcaat
    10981 gctatcggat gataccgggg ggttccggaa ggaattcctt gtttcccgaa ctgttaccaa
    11041 caaaattata acgatgaaca agtaaagacc ggttggtggc agtactgacc ggtctgtgat
    11101 ggatgattaa tttcacctta aatccgggtg ctgttaggat gctgacattt ttcgggtaca
    11161 ccattcagat tatgcaaata aagatccaat caaaaaataa acatccctga atataatata
    11221 ttcatagcat ccataactca tcctgggcga taactaaacc atttttgagt aataaatgaa
    11281 acaactgcaa cactcttatt atttctggaa aacgcctcgc aaaaacaaaa caacatgcaa
    11341 aaatccgctt tttttaaatg attttccggc gtaattttcg cacatacatt aatgagtaat
    11401 ataggtaagg tggtggtaat ggcgatgaat acggtttttc ttcatttatc agaagaggca
    11461 attaaacggc tgaacaaact tcggggctgg cgtaaggttt cacgatctgc aattttacgc
    11521 gaagcggttg agcaatatct ggagcggcag caatttccgg tgcgtaaagc aaagggaggc
    11581 aggcaaaagg gggaggtagt tggtgttgat gatcagtgta aggagcataa ggaatgaaaa
    11641 aaatttttca ttcttgaata taaaaaaaca gatgccctta ttctggtatt aatacaaggc
    11701 tgttttactt gaacttataa taactgcaac tgttacatcg tatctggaaa acgcctcgca
    11761 aaaataaaaa atgatgcgta aatgagcttt ctttaaagat tcggcaaggg tacttttctc
    11821 tccatagatt caaaaatgat attggcgaga tatttatgaa ctctctggca ggtatagata
    11881 tgggcagaat tttactcaat ttatcgaatg aggtgattaa gcaacttgat gatctcgagg
    11941 tgcagcgtaa tcttcctcgt gcggacctat taagggaagc ggtagatcaa tacctgataa
    12001 atcaatcgca aacagcaaga accagtgttc ctggcatctg gcaagggtgt gaggaagatg
    12061 gtgtcgaata tcagcgtaag ctgcgcgagg aatggtaatg ggaaaaaatg atgtcaatca
    12121 gattgctgac aacgtgcgcg ttgttc
//
LOCUS       DAZFRJ010000136        11740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000136 DAZFRJ010000000
VERSION     DAZFRJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.137"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..193
                     /locus_tag="ACHKUN_003394"
     CDS             <1..193
                     /locus_tag="ACHKUN_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661600.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHLHT
                     CSPHQKFILKLLNNHFPAN"
     gene            310..1608
                     /gene="kgtP"
                     /locus_tag="ACHKUN_003395"
     CDS             310..1608
                     /gene="kgtP"
                     /locus_tag="ACHKUN_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO6661601.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHKUN_003396"
     CDS             complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHKUN_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO6661602.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHKUN_003397"
     CDS             complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHKUN_003397"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO6661603.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHKUN_003398"
     CDS             complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHKUN_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO6661604.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHKUN_003399"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHKUN_003399"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO6661605.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHKUN_003400"
     CDS             complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHKUN_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO6661606.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHKUN_003401"
     CDS             7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHKUN_003401"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6661607.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHKUN_003402"
     CDS             complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHKUN_003402"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO6661608.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9607..9990
                     /gene="grcA"
                     /locus_tag="ACHKUN_003403"
     CDS             9607..9990
                     /gene="grcA"
                     /locus_tag="ACHKUN_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO6661609.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHKUN_003404"
     CDS             complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHKUN_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO6661610.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHKUN_003405"
     CDS             10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHKUN_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6661611.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2921 a         2932 c         3036 g         2851 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgcctg atgcgacgct
       61 gcgcgtctta tactcccaca tatgccagat tcagcagcga atacggcttc cccaacttgc
      121 ccacctccat acgtgttctc ctcaccagaa atttatcctt aagctcctca ataaccattt
      181 tcctgctaac taaattcatg gttaaggttg cataatgata cgcaacaaat gtataacatt
      241 ttctttacca aaagaaataa acaaaagcga ccgacaaaag catcggatta cggcaggaga
      301 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgataca
      361 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      421 ttctatgtct actcgttctg ttcactctac ttcgcccaca ttttcttccc ttccgggaac
      481 acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct gatgcgccca
      541 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg
      601 ttatcggtgt gtatgatgtg tttcggatcg ctggttatcg cctgcctccc aggttatgaa
      661 actataggta cgtgggctcc ggcattattg cttctcgctc gtttatttca gggattatcc
      721 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      781 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact gctagcccta
      841 ctggttgtcg tggttttaca acacaccatg gaagacgctg cactcagaga gtggggatgg
      901 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcaa
      961 ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1021 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1081 tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1141 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1201 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1261 ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc ctcgccttat
     1321 gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac atcaatcagt
     1381 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggcctgtca
     1441 tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt gtcgctgaaa
     1501 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1561 ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg acgggtcagt
     1621 tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc cttccagctc
     1681 cagccgctcc cttcggggcg gctatcccaa agctcttttg acgcccccaa actgacagag
     1741 aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg tgaatattca
     1801 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1861 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga
     1921 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     1981 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag tttacgaacc
     2041 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2101 tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg tggatcgtgg
     2161 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2221 atcaacatcc acttatcatc aacccacatc ccttttaggt gataggtgtt gtcgtcatct
     2281 ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2341 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2401 tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2461 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtatggcgta
     2521 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt gttcaacaga
     2581 ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc ggcatcgccc
     2641 tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc gttcttgatt
     2701 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2761 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat aagatgataa
     2821 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     2881 taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacgg
     2941 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcgg
     3001 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3061 tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc ttcatacaac
     3121 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3181 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3241 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3301 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3361 gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc cgattaagtg
     3421 gtcaacattt ccagtacctt actcatgatt cctcgcgttg ggcaagattt agcgtaagcc
     3481 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gctagcgcca
     3541 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg tgatctcgcg
     3601 tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg agatccgagc
     3661 gaaccagcac agcaaattcg gcatcgatgt tatcaggatc ggaaatcgca cgcgtgacgc
     3721 cgaggatctc ttccgtttga tcaatacgtc gtaccgctac aaacgccatt tcccgatcgt
     3781 agtcgatctg tgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3841 agcggtaata aagatcttct ttggtgactc gcgaaatgaa ttgctgaagt tgtggctcat
     3901 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccattctt
     3961 ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct tcaaacggcg
     4021 agatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4081 tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc accagaagct
     4141 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg tgcacgaatc tttttacttt
     4201 tgatcccctg aataaccaga tagcgggcca ggttcatgtt cagcggcggt aacgcgacga
     4261 cggcttgatc ttcaggacgc cactccacac cgccttcgcc cagcatgatc aacggcccga
     4321 aaaccggatc gtgctcaacc acaacccgca actcctgagc accagcacgg ttagccatac
     4381 tttgcaccaa caggccgtgg acccgtgcct gcggccaggc catttttacg cgatcgaaaa
     4441 tggcgtttgc cgcttgctgg acttcattgg ctgtacgcag gtagagcatg acgccctgaa
     4501 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4561 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtgggg agcgtgttca
     4621 tgccatatgc ttgcaggata ggctgaactt catgggtatc gagtgacgtt gctccttcgg
     4681 caatcgcctg ttgcaacaga agatgcgctt ctgcggtatt ggaggtcaga ttgctgggca
     4741 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4801 cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg aataatcgtc
     4861 gtgcctcttg cgaggaatgt tcgccacacc agttcgtcag cagagaaaca tacttgctgc
     4921 ggggatgatg ctttaccgct tcaattaata cttgcgcgct ttctgttgcg ggagcagcgg
     4981 cgctgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc agaatgtcca
     5041 gcgttttaat atagtgctca ctgctggcgt catcgcgcag atcgagcggg ttagagattg
     5101 ccacatgtcc tggcagtgca tcgcgcagtt tctggcaggt ttcttcgctt agtgttgcca
     5161 gcttgccatt gcgtgaccat aaggcatcaa gcgccagcgc ggcaggcgca gcaccgttgc
     5221 tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctaagggtt tccaccgccg
     5281 aaaacagttc gtgggtgtcc tgtacccgca acaaaccggc acgctgaata gccgcatccc
     5341 acgccggatc cattcctgcc gtggtgttca acagacgttg agccgccggg ctacgtccgc
     5401 ttttaatcac cagaatcggt ttgttgcgag aggcactacg ggccgccgaa acaaagcgtc
     5461 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgtgcca
     5521 gatagtcaag caattcatca acgtcgatat ccaggctgtc gccgagcgca ataaagtagg
     5581 aaaagcccat cttacgctgt tgcgcccagt cgaggatggt gttggagacg gcagccgatt
     5641 gcgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5701 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5761 gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata atgcaggttt
     5821 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     5881 aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     5941 acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6001 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgacgcgcca attaccgcta
     6061 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6121 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6181 gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa ccctgctgcc
     6241 gccccggtat cgctggccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6301 cctgcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc gacggaaatg
     6361 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6421 ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat cacttcccgt
     6481 tgctcatcag catacgaagc gggaaagacc accattggct gatagtccgg gttttgcacc
     6541 aactccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6601 aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6661 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6721 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6781 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacac cgttttcggt cataagagag
     6841 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     6901 attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg ccattaagca
     6961 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7021 gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7081 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7141 cccagaagtc gatcaccaca ggtagatcat ccttcagcaa tttgtcgagt gtttcaccgg
     7201 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7261 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7321 taactaacct cgggatgagt aaggagactt atcggcaacg acgccggtat gtttctaata
     7381 tgttacatat tatcggggac attgttccag acaacaatct gttttattca ataggtatga
     7441 tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7501 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7561 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7621 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7681 cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc gtggcgcacg
     7741 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7801 aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7861 ggcgaaaatg catgtcaggc acttttccag agccgtccgg aagcgattgt tcgcgcctgg
     7921 tttatccaga gtgtaacgcc gcgttttaaa gaagccttgc gctggatggc agcaaaccgc
     7981 aaagcgtacc atgtggtgga tgaagcggaa ttgacaaaag cgtcaggcac ggaacatcac
     8041 ggcggcgttt gcttcttgat caaaaagcgt aacggtacaa ccgtgcagca gtgggtaagc
     8101 caggcaggcg cgcaggattg cgttctggca ctggaaaacg aatctaaccc gcataacctg
     8161 ggtggcatga tgcgcagctg cgcgcacttc ggcgtgaaag gtgtcgtggt gcaggatgcg
     8221 gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8281 ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc aggttacacc
     8341 gtagtgacca cttccagcga gcagggtaaa ccgctgttca aaactagtct gccagcgaaa
     8401 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8461 gatctgcgcg tgaagattga cggtacgggc aacgttgccg ggctgaatat ctctgtcgca
     8521 accggcgttt tgcttggtga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8581 gcaactctgc catccggcat ttccccgcaa atttactcac tctctgccgg taatactggc
     8641 atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag cacaaaatgg
     8701 ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc tttcagtaca
     8761 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgtgatcc ccacaacaaa
     8821 aacaccacgc cttcgcggtg ctggttaatc aggctgatga ctttatcagt aaacgtctcc
     8881 caaccgaggc tggcgtggga atgcgcctga cctgcgcgaa ccgtcaacac agtattgagc
     8941 agcagaacgc cctgacgcgc ccagctttcg agatagccat gattagggcg ggtgaagccc
     9001 ggaatagtat tttccagctc tttatacata ttcaataatg acggaggaat ggcaatgccg
     9061 ggacgaacgg aaaatgccag accatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9121 agaatcacca ctttaacgtc acccaactcg gtaaagcgga acgcgttaaa gacatctttt
     9181 tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9241 agaaaataag gttgttgctt ctcttcagcc agcacgtcat gccaggttaa ttcgttagcc
     9301 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9361 aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg taaaatttca
     9421 cacccgcata agttgatgta aaacaataaa atccatttac gaccacaatc gatgtgtggt
     9481 ttttattgat ttaaatcaaa gaatcaaggg tggttgagga gtatatatac actcaagcaa
     9541 caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt gccggggagg
     9601 catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct gctgaactct
     9661 ttctggctgc tggacagcga aaaaggcgaa gcacgttgca tcgttgcaaa agcaggttat
     9721 gcagaagatg aagtggttgc agtaagcaaa ctgggtgaca ttgaataccg tgaagttcca
     9781 gtagaagtga aaccagaagt tcgcgttgaa ggtggtcaac acctgaacgt taacgttctg
     9841 cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca gctgaccatc
     9901 cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca gcgcgacgtt
     9961 atcgctcgta cctttactga aagcctgtaa ggcttaagta gcaagttact aagcggaaga
    10021 gggcgacctc ttccgctttt tttcgttaat agaaaatgcg taccgcgcaa tagaccagca
    10081 acagggcaag cacgatattt aactggcgac catactggcg aaacaatcgc tgaaacagat
    10141 gccccgccag cgcccagcac acattgccaa acgtcccaat catcgccagc aaaacgctga
    10201 cgccaactac ccagcttagc gcctgtgttt gcggcagaac aaacgtcgac agtgccgtaa
    10261 caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc caaaagctga
    10321 ttggttttgc ctgaagtccg tcttcctttg ttgggctggt ggcgattttc cacgccagcc
    10381 agacaatata tgccgccccc gcccaactca aaaggtgtac cgctgccggg tcaatcactg
    10441 ccagtgaaaa tgaaatgccc gcacacagta acatcacaat caaaaatccc agactcatcc
    10501 ctgccagcac gcgggtactt tgacgaaatc catgcgacgt agcagagcta agggcgagaa
    10561 tattgttcgg tcctggcgtc atagcggtaa tcagggtgta agtccaaaaa gcacttaaaa
    10621 gggtcggtgt cacttgttat ctcctctgtt ttggtagaca gtacccgaca cggaacattt
    10681 aaaaaaatga atgtttttat attctagatt cgatattttc gataggtttg gggttatgga
    10741 tctgcgccgt tttattacgc ttaaaaccgt ggtggaagag ggttcctttt tgcgagcttc
    10801 gcaaaaattg tgctgtacac aatcgacggt gacttttcat attcagcagc ttgagcagga
    10861 gttttcagtc cagttatttg agaaaattgg ccgacggatg tgccttaccc gcgaaggaaa
    10921 aaagttgctg ccgcacattt atgaacttac ccgagtgatg gatacgcttc gtgaggctgc
    10981 caaaaaggag tcagatccgg acggggaact acgcgttgtt tcgggcgaaa cgttgctctc
    11041 ttaccgaatg ccgcaggtgt tgcagcgttt tcggcaacgc gcaccaaaag tgcgtttatc
    11101 gttgcagtcg ctgaactgtt acgtgatccg tgatgcgcta ctgaatgatg aggccgatgt
    11161 cggcgtcttt tatcgcgtag ggaatgatga tgccctgaac cgacgagagt tgggtgaaca
    11221 atcgctggtg ctggtggctt caccgcaaat tgcagatgtc gattttactg agccgggaag
    11281 acataacgcc agtagcttta ttatcaacga accgcaatgt gtcttccggc agatatttga
    11341 gagcacgctg cgtcagcggc ggatcacggt ggaaaacacc attgagctgt taagcattga
    11401 aagtatcaaa cgttgtgtcg cggcgaatat cggtgtcagt tacttgcccc gctttgcggt
    11461 ggaaaaagag cttgagagcg gagaattaat tgaattgcct tttggcgaac agtcgcagac
    11521 cattacggcc atgtgcgcac accatgctgg aaaagcggtt agcccggcga tgcacacttt
    11581 tattcagtgt gttgaagaga gttttgtggc tggataaaaa taatgtcggg attaaacccg
    11641 gcatttaact tgtcgacaaa gtgcgcttta ttcatgccgg atgcggcgtg aacgccttat
    11701 ccggcctaca agatcgtgca aattcaatat attgcatgag
//
LOCUS       DAZFRJ010000137        11578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000137 DAZFRJ010000000
VERSION     DAZFRJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.138"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            41..2466
                     /gene="ynfE"
                     /locus_tag="ACHKUN_003406"
                     /pseudo
     CDS             41..2466
                     /gene="ynfE"
                     /locus_tag="ACHKUN_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            2527..4950
                     /gene="ynfF"
                     /locus_tag="ACHKUN_003407"
                     /pseudo
     CDS             2527..4950
                     /gene="ynfF"
                     /locus_tag="ACHKUN_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
     gene            4961..5578
                     /gene="ynfG"
                     /locus_tag="ACHKUN_003408"
     CDS             4961..5578
                     /gene="ynfG"
                     /locus_tag="ACHKUN_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO6661612.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5580..6434
                     /gene="ynfH"
                     /locus_tag="ACHKUN_003409"
     CDS             5580..6434
                     /gene="ynfH"
                     /locus_tag="ACHKUN_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO6661613.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6477..7091
                     /gene="dmsD"
                     /locus_tag="ACHKUN_003410"
     CDS             6477..7091
                     /gene="dmsD"
                     /locus_tag="ACHKUN_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO6661614.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7250..8542
                     /gene="clcB"
                     /locus_tag="ACHKUN_003411"
     CDS             7250..8542
                     /gene="clcB"
                     /locus_tag="ACHKUN_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO6661615.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8495..9190)
                     /gene="bioD"
                     /locus_tag="ACHKUN_003412"
     CDS             complement(8495..9190)
                     /gene="bioD"
                     /locus_tag="ACHKUN_003412"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6661616.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9315..10535)
                     /gene="mlc"
                     /locus_tag="ACHKUN_003413"
                     /gene_synonym="dgsA"
     CDS             complement(9315..10535)
                     /gene="mlc"
                     /locus_tag="ACHKUN_003413"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO6661617.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10670..11563)
                     /gene="ynfL"
                     /locus_tag="ACHKUN_003414"
     CDS             complement(10670..11563)
                     /gene="ynfL"
                     /locus_tag="ACHKUN_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6661618.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
BASE COUNT         2821 a         2999 c         2958 g         2800 t
ORIGIN      
        1 tataaacatt tatataacga taaagaacag ggagtgagtt atgtccaaaa atgaccgaat
       61 ggtgggcatc agccgcagaa cactcgttaa atcaaccgcg ataggttctc tggcgctggc
      121 tgcaggcggt ttttctttac cgttcaccct gcgcagtgca gcggcaacgg tacaacaggc
      181 cagcgaaaaa gtgatctggg gtgcctgttc cgtcaactgt ggtagccgct gtgcacttcg
      241 tctacatgtt aaagataatg aagtgacctg ggtggaaact gacaataccg gcagcgatga
      301 gtacggcaac catcaggtac gcgcctgttt gcgcggtcgc tccatccgcc ggcgtattaa
      361 tcatcccgat cgcttgaatt acccaatgaa acgcgtgggt acacgcggtg aaggcaaatt
      421 cgaacggatt agctgggatg aagccctgga tactatcgcc agtagcctga aaaaaaccgt
      481 cgaacaatat ggcaatgagg ctgtatatat tcagtactct tcggggattg ttggcggcaa
      541 tatgacccgc tcttcgccat cagcctcggc ggtcaaacgc ctgatgaact gctacggcgg
      601 ttcactcaat cagtatggct cctacagcac cgcgcaaatt tcctgtgcca tgccctacac
      661 ctacggcagt aatgatggca acagcaccac ggatattgaa aacagcaagc tggtcgtgat
      721 gtttggtaac aacccggcag aaacccgcat gagcggcggt ggcatcactt atcttcttga
      781 aaaagcgcgc gagaaatcga acgccaaaat gattgttatc gatccgcgat ataccgatac
      841 ggctgctggt cgtgaagatg aatggctccc tattcgcccg ggcaccgatg ccgcgctggt
      901 tgcgggtatt gcctgggtat tgattaacga aaatctcgtt gatcaacctt ttctcgataa
      961 atactgcgtc ggctatgacg aaaaaacctt acccgcagat gcacccaaaa atggtcacta
     1021 taaagcctat attcttggtg aaggtgatga taacacagcg aaaacgccgc agtgggcttc
     1081 gcaaattacc ggtatcccgg tagaccgtat catcaaactg gcgcgtgaaa ttggcacagc
     1141 aaaacccgct tatatctgcc agggctgggg gccgcaacgc caggcaaacg gcgaactgac
     1201 tgcacgcgct attgctatgt tacctatttt gacgggcaat gtcggcatca gcggcggaaa
     1261 tagtggcgcg cgtgaatcga cctataccat taccatagaa cgcctgccgg tgctggataa
     1321 tccggtcaaa acgtcaatct cctgcttcag ctggacagat gctatcgatc atggtccgca
     1381 aatgacggca atccgcgacg gcgtccgcgg caaagataaa ctggatgtgc ccattaagtt
     1441 catctggaac tacgcgggaa ataccctcgt taatcagcat tctgacatca acaaaacgca
     1501 tgaaattctg caggacgaat cgaaatgcga aatgattgtg atcatcgaaa actttatgac
     1561 ctcatcggca aaatatgccg acattctgct gccagacctg atgaccgttg agcaggaaga
     1621 tattattcct aacgactacg ccggtaacat gggatatctc attttcctcc agcctgtcac
     1681 cagcgaaaaa ttcgaacgca aaccgattta ctggatcctg agtgaagtcg cgaaacgtct
     1741 tggacctgac gtctatcaaa agttcacaga aggtcgcacg caggaacaat ggttacaaca
     1801 tctgtacgcc aaaatgcttg ccaaagatcc ggcgttaccg tcttacgacg aactgaaaaa
     1861 atgggtatct ataagcgtaa agatcccaat ggccattttg tcgcctacaa agcatttcgt
     1921 gacgaccccg aggcaaatcc acttaaaacg ccttccggta agattgaaat ttattccagc
     1981 aggctggcgg aaattgcccg tacctgggaa ctggaaaaag atgaagtgat aagcccattg
     2041 ccagtttatg cctcaacctt tgaaggctgg aactcccctg aacgtagaac cttcccactg
     2101 caactgtttg gtttccatta caaatcccgt actcactcga cctacggcaa tattgatctc
     2161 ctgaaggctg cctgccgtca ggaggtgtgg atcaacccta tagatgcgca gaaacgtggg
     2221 attgccaacg gcgatatggt gcgggtgttt aaccatcgtg gcgaagttcg gctaccagcc
     2281 aaagtaacac cacgtattct ccctggagtt agcgctatgg gccagggagc ctggcacgag
     2341 gccaatatgt ctggcgataa aatcgaccat ggcggctgtg tgaatacgct aaccactctg
     2401 cgcccttcac cactggcgaa gggaaacccg cagcacacta atctggtcga gatcgaaaaa
     2461 atataaccca cgacaaccat aaattctggc atgacatttt gtttgaaaag caataagtga
     2521 gtaatgatga aaatccatac cacagaggcg ctcatgaagg ctgaaatcag ccgtagaagt
     2581 ctgatgaaaa cctccgcact tggcagtctt gcgctggcaa gcagtgcttt cactctgcca
     2641 ttttcccaaa tggtccgggc ggcagaggct ccggtagaag agaaagcggt ctggagttcc
     2701 tgcaccgtta actgcgggag ccgctgtctg ttacgtttgc atgtgaaaga tgacaccgtg
     2761 tactgggtgg agtctgatac gacaggtgac gacgtctacg gtaaccatca ggttcgagcg
     2821 tgtttacgcg ggcgctctat ccgccgacgg atgaatcatc ctgataggtt gaaatatccc
     2881 atgaagcgcg tcggcaagcg cggtgaaggt taatttgaac ggataagttg ggacgaagcc
     2941 ctggatacca tcagtgataa tcttcggcgg atcctgaaag attacggcaa tgaggctgta
     3001 catgtcctgt acggaacagg cgtagatggc ggaaacatca ccaactcaaa cgtcccgtac
     3061 cgtctgatga actcttgcgg tggttttctc agtcgctatg gcagctacag taccgcacag
     3121 atcagtgccg caatgagtta tatgttcggt gccaatgatg gcaacagccc ggatgatatc
     3181 gccaatacga aactggtcgt tatgttcgga aataacccgg cagaaacgcg gatgagcggc
     3241 ggtggtgtca cttactacgt cgagcaagcc cgcgaacgtt caaacgcacg catgatcgtc
     3301 atcgatccac gttataacga cactgctgcc gggcgtgaag atgaatggct gcccattcgc
     3361 cctggcaccg atggcgcact ggcctgtgcg attgcctggg tactgattac tgaaaacatg
     3421 gtcgatcagc catttctcga caaatattgt gttggttacg atgaaaaaac gctgcccgcc
     3481 aacgcaccac gtaacgcgca ttataaagcc tatattctgg gcgaagggcc tgacggcata
     3541 gctaaaacgc cggaatgggc agcaaaaatc accagcatcc cggcagaaaa aattatccag
     3601 ttggcacgag agatcggttc agcaaaacct gcttatattt gtcagggttg ggggccacaa
     3661 cgacattcca acggcgagca aacgtcccgc gctattgcca tgctttccgt tctcaccggt
     3721 aacgtcggca taaacggcgg caactcaggt gtacgcgaag gtagctggga tctgggggta
     3781 gaatggttcc cgatgcttga gaatcctgtt aaaacgcaga tttccgtctt tacatggaca
     3841 gatgccatcg accatggtac ggaaatgacc gcgacccgtg atggtgttcg tggaaaagaa
     3901 aaactggatg tccccatcaa gtttttatgg tgctacgcca gtaacacatt gatcaatcaa
     3961 catggcgaca tcaatcacac ccatgaggtg cttcaggatg acagcaagtg cgagatgatt
     4021 gttggcattg accacttcat gacggcctcg gctaagtatt gcgatatcct gttgcccgac
     4081 ctgatgccaa cagagcaaga agaccttatc tctcatgaat ctgcagggaa tatgggctat
     4141 gtgatcctcg cccaacccgc aacctcagca aaatttgaac gcaaacccat ctactggatg
     4201 ctgagtgaag tcgccaaacg cttaggacca gacgtttatc aaacctttac tgaaggtcgc
     4261 agtcagcatg aatgggtcaa atatctccat gcgaaaacga aggaacgtaa ccctgagatg
     4321 cccgactacg aggagatgaa aacgaccggg atctttaaga aaaaatgccc ggaagaacac
     4381 tacgtcgctt tccgcgcatt ccgtgaagat ccacaggcaa acccgttgaa aacaccttcg
     4441 gggaaaatcg aaatttattc tgaacgactg gcgaagattg cagatacctg ggaattgaaa
     4501 aaagatgaaa ttattcatcc ccttcctgcg tataccccag gttttgatgg ctgggacgat
     4561 cccctgcgga aaacctatcc actgcagtta acgggcttcc attacaaagc gcgtacccac
     4621 tccagctacg gcaatattga tgtgttacag caggcctgcc cacaagaggt gtggatcaac
     4681 cccattgatg ctcaggcacg cggtatccgt catggcgata ccgtgcgggt atttaacaat
     4741 aatggagaaa tgctgattgc cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc
     4801 atcgggcaag gtgcgtggct taaagcggat atgtttggtg accgggtcga tcacggcggc
     4861 agtatcaata ttctgacctc tcaccgccct tcaccgctgg caaagggaaa cccgtcgcac
     4921 agcaatcttg tccagatcga aaaggtttaa ggagtagccc atgaccacac aatatggatt
     4981 ttttatcgat tccagccgct gtactggctg taaaacctgc gaactggcgt gcaaagattt
     5041 taaagatctt ggcccggaag tcagtttccg ccgtatttat gaatacgctg gcggcgactg
     5101 gcaggaagat aatggcgtct ggcaccagaa cgtgttcgct tattatctct ccatctcctg
     5161 taaccattgc gatgaccccg catgtacaaa agtctgcccc agcggcgcaa tgcataagcg
     5221 tgaagatggc tttgtggtgg ttgatgaaga tgtttgtatc ggctgccgct actgccacat
     5281 ggcttgtccg tacggcgcac cacagtacaa tgctgaaaaa gggcacatga cgaagtgcga
     5341 tggttgttat tcgcgcgtcg ccgaggggaa acaacccata tgcgtcgaat cctgcccgct
     5401 gcgtgcactt gagttcggtc caattgaaga gctgcgccag aagcacggca ctctggctgc
     5461 cgtcgcgcca ctgccgcgcg cgcatttcac aaaacccaat atcgttatca aacctaacgc
     5521 caacagccgc ccgaccggcg ataccactgg ttatctggct aatccggagg aagtgtaaaa
     5581 tgggaaatgg atggcatgaa tggccactgg ttatctttac cgtattgggt caatgcgtag
     5641 taggcgcgct gattgttagc ggaattggct ggttcgccgc caaaaatgac gcagaccgtc
     5701 agcgtattgt ccgtggcatg tttttcctct ggctgttgat gggcatcggg tttattgcct
     5761 ctgtcatgca cctgggatcg ccactgcggg cgtttaactc gctgaatcga atcggagctt
     5821 ctggtctgag taacgagatt gctgccggtt ccatattttt tgccgttggc ggactatggt
     5881 ggctggtggc ggtcatcggt aaaatgccgc aagcgttggg gaaactctgg ttacttgtca
     5941 gcatggcgct cggcgtcatt ttcgtctgga tgatgacctg cgtgtatcaa atcgacactg
     6001 tgccaacctg gcataacggt tacactacgc tggctttctt cctgacggta ttgttgagcg
     6061 gtccaatact tgctgccgca attctgcggg cagcccgcgt tacatttaat accacaccat
     6121 ttgccatcat tagcgttctg gcgctgatcg cttgtgccgg ggtgattgtg ctgcaaggtt
     6181 tgtctctggc gtctattcac tcatccgtgc aacaagccag cgcactggta ccagattacg
     6241 cctccttaca ggtctggcgt gtggtattgt tatgcgcagg tcttggatgc tggctttgcc
     6301 cgctcattcg tcgccgtgaa cctcacgttg ccggacttat tctggggcta atcctcatac
     6361 ttggcggtga gatgatcggg cgtgtgctct tttatggcct gcacatgaca gtaggaatgg
     6421 ctattgcagg ttaacaaatt ggtgcggggc aacccgcaca tttcaggatg ttaggaatga
     6481 cccatttttc acagcaagat aatttttctg tcgcggcgcg cgtgttgggc gcgctgtttt
     6541 attacgctcc agagagtgcg gaagccgcac ctctggttgc ggtactcacc agtgacggct
     6601 gggaaactca gtggcctcta ccagaagcgt cattagcgcc gctggtgacg gcttttcaga
     6661 cacagtgtga agagactcac gcccaggcct ggcagcgttt gtttgtcggc ccgtgggcac
     6721 tgccgtctcc gccgtggggg tcggtctggc tggatcgcga atctgtgctg tttggcgatt
     6781 caacattggc acttcgtcag tggatgcgcg agaaaggcat tcagtttgaa atgaagcaaa
     6841 acgaaccgga agatcatttt ggttcactgc tgttgatggc tgcgtggttg gcggagaatg
     6901 gtcgccagac agaatgcgaa gaattgctgg catggcacct ttttccgtgg tcaacacgtt
     6961 ttcttgatgt ttttatcgaa aaagcagaac accctttcta ccgtgcactg ggtgaactgg
     7021 ctcgcttaac gctggcgcaa tggcaatcac aactgttaat tcctgtcgcg gttaaaccgc
     7081 tgtttcgata ggatcaccgt aatattgccg gatggtgacg attacgcgca atccggcaat
     7141 aataggttac agtgtcacgt ttttttatct cttaaagcac gcactgcttt tgcggctggc
     7201 ctcttttgcc gcaaaatagt cgcccgtgtt tcattgccca tttctgctca tgcatcatct
     7261 acacatctat ccggatctgc gcactatgtt ccgccgtctg cttatcgcaa cagtcgtcgg
     7321 tattctcgcg gcctttgccg ttgccgggtt tcgtcatgcg atgctgctac tggagtggtt
     7381 gttcctcaat aatgactccg gcagtctggt caatgcagcg acaaaccttt ccccctggcg
     7441 acggttgcta actccggcgc tcggcggact ggcggcgggt ttgttgctga tgggctggca
     7501 gaaatttacc caacaacgcc ctcatgcgcc gaccgattac atggaagcgt tgcaaaccga
     7561 tggacagttc gattacgcag caagcctggt taaatcgctt gcctctctgc tggtagtaac
     7621 cagcggcagt gcaattggtc gcgaaggtgc gatgattctt ttagctgccc ttgccgcctc
     7681 ctgttttgcc caacgtttta cgccacgcca ggagtggaaa ttatggatcg cctgtggggc
     7741 cgcggcggga atggctgcgg cctatcgtgc cccgcttgct ggcagtttat ttatagccga
     7801 agtgctgttt ggcactatga tgttggcctc tctcggcccg gtgattattt ccgccgtcgt
     7861 ggcattgctg gttagcaatc tgattaatca tagcgacgcg ttactctaca acgtacaact
     7921 ctcagtgacg gttcaggctc gtgactatgc gctgattatc agtacaggtg tgctggcagg
     7981 tctgtgcgga ccactgttgt taacgttaat gaacgcctgt catcgtggat ttgtgagtct
     8041 caaacttgcg ccgccctggc aactggcact aggcgggttg atcgtgggtc tgctttccct
     8101 gttcacacct gcagtgtggg gcaacggcta tagcaccgta caatcctttt taaccgcccc
     8161 accactgtta atgatcattg ccgggatctt cctttgtaaa ctgtgtgccg tgctggcgag
     8221 tagcggttcc ggcgcacccg gtggtgtctt tacaccgacg ctatttatcg gtcttgccat
     8281 tggcatgttg tatggtcgta gcctgggatt atggttccct gatggcgaag aaattacgct
     8341 tttactcgga ttgaccggga tggcgacact gttggcggca accacgcacg cgccgattat
     8401 gtcgacgttg atgatatgtg aaatgaccgg ggagtatcag ctactccccg gtttattgat
     8461 tgcctgcgtc attgcgtcgg taatttcgcg gacgttacac cgtgactcta tctaccgcca
     8521 gcacactgcg cagcatagct aagcggatgt attgccccag ttctcgctgt tcggcgcgcg
     8581 gcagataagg cagttcacca atgagcggtg ccggaagttt tttacccagc acatcaatga
     8641 tttccgcata atgcgccagt cctgggttga ttcggttagc cacccagcca atgagcggca
     8701 gcccgtcgtt ggcaatcgcc tgagctgtta gcagtgcatg gttaatgcaa ccttcctgaa
     8761 taccgacaac catcaacacc ggcaactgtt cctgcaccac ccattcagag agtggacgca
     8821 aatcattcat cagactgcgc cagccgccag tcccttctac cacgacatga tcgactttat
     8881 cggtcaggtt tgccaggccg tttgaaatga gggtgtaatt gattgggcaa ctgtgcgcca
     8941 cgctactttc ttcttcgctt aacgcgatag gattaactgc ttcataaggc agttcgatgg
     9001 ttgaaacact ctgcaacacc agggcatctt tattacgcag cccttcgggt gtctctttgc
     9061 tccccttcgc tacgggttta tatcccgcaa ccgtttttcc ctgggaggct aacgcttgta
     9121 gcaatgcgcg ggaaaccacc gttttcccta cagaagtgtc tgtaccggta ataaagaaac
     9181 gcttcagcat cactaactcc accgttatgc ttcacaaata taaaccagga aaataattaa
     9241 ccttgaaagt ctaagttatg ctttcctggc ccaaattgag atagcgcaaa ttttggtaga
     9301 acagttaaaa aatgttaacc ctgcaacaga cgaatcaaca aagaaccgtt atacatcgcg
     9361 tcttttacca gtgcagcgcc tgccatcgtg ccctggttag aaaactgagt actctcaacg
     9421 ctgatgtgct gactatacgc aggaagggcc tgctgacgga tgctgtctga gatgaccggg
     9481 aagaggatat ctgccgcttt acttaacggt gagccaatca gtattttttg tgggttaaat
     9541 aaattcacca tgatggcaag aatgcgccct acatgcgcgc ccaccccggt aatgatgtct
     9601 tttgccagca gatcgccgcg caatgccgcc tgacacaatg agtccacggt taacggttgt
     9661 ccatgtaaca tcgagctcat ggattgatta agacgcagct gtgccagctc aagaatactg
     9721 tccacactgg cgatggtttc gaggcagccg tgattcccgc aataacagcg tttcccatac
     9781 gggtcgacct gtgtgtggcc tatttccacg agactactgc tgcctgcgtg tagcagatga
     9841 ccatcggtaa tgacgcccgc ccccacgttg tgatcgataa ccacctgaat cacatcgcgc
     9901 gccccgcgtg aggcaccaaa caaggcctct gccatcgtcc atgcgctgat atcatgctga
     9961 atataaaccg gtacgccggt atgctgctcc agcgcctcgc cgagcggcat ctcttttaca
    10021 tcctcgtaga acggcatgcg atgtacaata ccattttccg tatcaataat tcccggcaag
    10081 gttatggcaa tcgaagttag acgctcaagt tttttctggt ggcgaataaa aaactgatcg
    10141 atatgggaaa tgatacgatc cagcaatggc gagtcatctt ttaacgccag ttcctgcgac
    10201 tcttccacca ccagtttgct gctcagatcg cgcagagcaa ggaaaatctc cccgcgacta
    10261 atgcgcagag aaagatagtg ccaggcttca gtttcaacca ccagccccac cgccggacgg
    10321 ccacggttcc ccgcttcttt gatttccagc tcttgcacca ggtgtgcttc gagcatctca
    10381 cggacaattt tagtgatact ggcaggagcc agctgcgcca gacgggaaag atcgatacgc
    10441 gagactggac caagctgatc aatcaggcga taaaccgcgc ccgcgttggt ctgctttatt
    10501 tgatcaatgt gcccaggctg gttttcagca accaccgcat actccctata ttttcgcgct
    10561 ccgaaataat ctgtaggcta tggtgaagca cttcaatacg tgtcgtcaaa tttttactta
    10621 ggcatgtgat taacagcaca tttttcgggc tttttcgctg aaatttccct cacctgagag
    10681 cattcagcag atgaatacga aagttacgcg cagccggact ttgttcatga tgtttcggcc
    10741 agaccaacca catttcagaa accgcatcct cttcagcaat cggcacccag cgcatttcgt
    10801 tgagctgaac acgtttaaat gaagcaggca aaattgaaac acccagaccg gcggaaacca
    10861 gaccgatgat ggtcattgcc tcgcccacct cctgagtgat gacgggcgtc aaatggtaac
    10921 gtcgcatcag cccgagaata tcgtcataca gccctgtccc gacgtgcgga tcaaaaaaga
    10981 cgaagggttc tttcgccagt tcagccagcg ttacattcgg gttatttgcc aggggatgat
    11041 cgtgcgggat catcgccata agcggttcat ggacgatgac tgcgtgttca agcgtctccg
    11101 gtaacgctgt gtaacgcagc aatcccatat ccagcgttcc ttcaatgagc ggagcgattt
    11161 gctcacgagt gttcatttcg cgggtttgta aatggacatc gggataatca cggcgaaaca
    11221 gcgataacgt atcggacacc gcccgaataa aaggagcgga cgaagtaaaa ccaatgcgca
    11281 actcccccgc ttccccctga tgcagtcttt cagcgcgagc ggcagcgtca tctaccatag
    11341 acaggatttg ccgactatct gcaagaaact gttttcctgc tgccgtcagc aatacactgc
    11401 gattagttcg tgccagcagt cgggcaccaa tttgttgctc cagtgcctga atctgctgac
    11461 ttagcggtgg ttgcgaaata ttcaggcggg cagcggcgcg cccgaaatgc agctcttccg
    11521 caacagcaac aaagtaacgc agatgacgaa gttcaatatt catatttaaa acatctta
//
LOCUS       DAZFRJ010000138        11466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000138 DAZFRJ010000000
VERSION     DAZFRJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.139"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..747
                     /gene="yijF"
                     /locus_tag="ACHKUN_003415"
     CDS             130..747
                     /gene="yijF"
                     /locus_tag="ACHKUN_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO6661619.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKUN_003416"
     CDS             complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHKUN_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO6661620.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKUN_003417"
     CDS             complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHKUN_003417"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO6661621.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKUN_003418"
     CDS             complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHKUN_003418"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO6661622.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5248..5382
                     /locus_tag="ACHKUN_003419"
     CDS             5248..5382
                     /locus_tag="ACHKUN_003419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661623.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKUN_003420"
     CDS             complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHKUN_003420"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO6661624.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKUN_003421"
     CDS             complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHKUN_003421"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO6661625.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKUN_003422"
     CDS             9393..9710
                     /gene="metJ"
                     /locus_tag="ACHKUN_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO6661626.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKUN_003423"
     CDS             9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHKUN_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO6661627.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10647..11322
                     /locus_tag="ACHKUN_003424"
                     /pseudo
     CDS             10647..11322
                     /locus_tag="ACHKUN_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2812 a         3194 c         2879 g         2581 t
ORIGIN      
        1 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
       61 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
      121 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
      181 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
      241 gccatcgcag acggtgccag acagcaaatt ggcagcacgc tattttacga tccggcgtat
      301 atgcagctta cctatcctgg cggtgatgtc ccgcaagaac gcggggtatg ttccgatgtg
      361 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggtgca cgaagatatg
      421 gcgaagaatt ttgccgagta cccgcaaaaa tggcagttaa agcgcccgga cagcaacatc
      481 gatcaccgtc gggtgcctaa tctggaaacc tggtttaccc gccacgataa aacacgcccc
      541 atcagcaaga accccagtga ctatcaagcg ggcgatattg tctcctggcg actggataac
      601 ggactggcac atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccgctggtg
      661 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
      721 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
      781 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
      841 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
      901 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
      961 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
     1021 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
     1081 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgacaa taaatccacg
     1141 cgcggatgac gggcatacag acgtttagca acaatcgcgc tcgcccccca actgacgccg
     1201 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
     1261 accaaaaata agccgaaagc ggcaatcagt atcgcgaaat attgcccacg tcgcaggcgt
     1321 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gcttaggatc
     1381 gccaccttcc ccgctccgcc gctgaccagc gcccactgcg ccaggccaac catcccgcag
     1441 gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg tcgggcgcat tccgcgacca
     1501 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
     1561 tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa tccagctata actccagata
     1621 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc gacagacatg
     1681 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
     1741 tgcctgaaag gcagtcgctg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
     1801 atcactttca cccatgccgc cacgaagtct ttaacaaact tctcgtgagc atcgctgctg
     1861 gcgtaaactt cagccaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
     1921 ctggcagtgt atttcacttc gccggtttcg cggtcacggc cttcaaacag ctctttcgat
     1981 tcgtcggtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
     2041 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc catcgaagtt ggcacccagt
     2101 acacgcatcc cgcccaccag cgcggtcatt tccggcgcgg tcagggtcag ttgctgggct
     2161 ttgtcgatca gcagtgactc ggtggtggaa acatccagac gagcgcgata gttacggaaa
     2221 ccgtcagcaa ttggctccag cagctcgaac atctcaatgt cagtctgatc ctgacgcgca
     2281 tcaacgcggc ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
     2341 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
     2401 ttctggattt tctccaaaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
     2461 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
     2521 aaggtagaag cagaagccca ggccaccgat accagctcac taacagacag accagaatcc
     2581 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
     2641 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
     2701 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
     2761 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
     2821 aggtcggtca ccagcatcgt cggtttacgt ttcttcgacg gatcaaacgg atccgggata
     2881 atttccggtg cgtctaccgc ttcgaactgg attgcgccag ccgggctgcg ggtctgtacc
     2941 cactcatact tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
     3001 cagactactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
     3061 gcccaaccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
     3121 gtcggaccgg caccgtgggt tttacccagc gtatgaccac ccgcaataag cgccacggtt
     3181 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
     3241 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat ctcggttgca
     3301 cccagcggtg ctttcgccag cgcttccgga tgacggtggg tcagccaggc tttttcatca
     3361 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
     3421 gtacggaagc cggagttttc cagcgccacg ttacccgcga ggataaacag gtcggcccag
     3481 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcgagg
     3541 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
     3601 ccgcgtccat cgattgaacg gtaagtcccc gcgccgtgcc aggccatacg aataaacaga
     3661 ccggcgtagc tgccccagtc ggcaggccac cacggctgag attctgtcag cagggctttc
     3721 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
     3781 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
     3841 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
     3901 aacgggcatt tgccagtggc tgtcgtgtta tgaatatcgt ctgacgtgct catcaatgtg
     3961 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
     4021 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtagatcaaa
     4081 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaactgctt tataaaaaag
     4141 ggttttagat tcctattctt caaggaagga gggcgaaaac aaagctgaca accttatttt
     4201 tacgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cacagtgtga
     4261 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
     4321 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
     4381 taaaatcttc accatatcca tggcaatatt cgcgcccacc agcttgcgcg tttcagcatc
     4441 gtcatccagc ccgtcaaaca tttgtgccat ccacgccgga atacgcacgt tggtcatatc
     4501 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa atccccggaa taatttccac
     4561 atcaatgccc gccgatacgc agcggtcacg aaaacgcagg tagctttcga catcaaagaa
     4621 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcaggt tgagcaaatc
     4681 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
     4741 atctgccact tcttttaaca gcgtcaccag gtcagaagca tacatttccg gcttaccact
     4801 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
     4861 gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
     4921 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
     4981 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
     5041 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
     5101 gttaatctgc ccctggactt ctgccaggct ctgattcagg gcatcccgct ggctggcgtg
     5161 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
     5221 tatccattta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
     5281 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
     5341 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
     5401 gcttcttgcc ggattcgtcg taaacgcttc atccggccta atcctatgat aattcaatat
     5461 atttcattag aaaccataga cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
     5521 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
     5581 cccagcgcca ggcccacgga tcaccagcgg attatcgcgg taccagcggc tttcgatggc
     5641 aaagacgtta tcgcatggca gcagtgatgc cagcggatgg tcttcacgca ccgcttccac
     5701 gcccacacgc gctttgccat tggcatcgaa acgcgccacg tagcgcagca ccagccccat
     5761 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
     5821 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
     5881 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
     5941 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
     6001 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
     6061 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
     6121 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
     6181 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
     6241 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
     6301 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
     6361 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
     6421 gtccagcccg tcatagctca acaggctgcg gcggctgtcc accacacctg ccagcacaaa
     6481 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
     6541 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
     6601 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
     6661 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
     6721 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
     6781 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
     6841 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
     6901 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
     6961 tttgaaatcc tgactgacgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
     7021 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
     7081 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
     7141 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
     7201 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
     7261 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
     7321 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
     7381 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
     7441 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
     7501 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
     7561 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
     7621 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcgg cttcttcagc
     7681 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
     7741 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
     7801 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
     7861 gacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagctgacg
     7921 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
     7981 ccattttcca agtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
     8041 agcgtctcgg aaatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
     8101 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
     8161 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
     8221 attgcgccaa agcctttttg ctgacgcgcg gcaatttcat gcccctgatt ttccggcagc
     8281 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
     8341 gcgttgcgct gcgccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
     8401 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg cccaccaggc aagttcagtg
     8461 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
     8521 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
     8581 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
     8641 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
     8701 ttgggttttt ctgccagtgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
     8761 acgcgatagc aaccgcgttt cgccaggctg tcgaacaggc gatagctacc gccgtagcag
     8821 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcattac caggtgaatc
     8881 gcggacatgc cggtattagt aagtactgca ccagcaccgc cttccagttc tgccagcgca
     8941 cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc gcgcggttca
     9001 ttaaatccgg taaagttata tgtgctggaa agatggatcg gtgggacaac gcaaccatac
     9061 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
     9121 tgaagttccc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
     9181 gacgtcaata catctggaca tctaaacttc tttgcgtata gattgagcaa atcccaaata
     9241 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
     9301 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
     9361 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
     9421 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
     9481 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
     9541 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
     9601 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
     9661 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcggaaatg
     9721 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
     9781 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9841 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
     9901 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccgcaaa
     9961 ctggcgacat catctttcaa atctctcgct catcgcaaag taaagcgatc caactggcaa
    10021 cccattccga ttacagccac accggtatgc tggtgatgcg taacaaaaag ccctacattt
    10081 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggattgcc catggtgaaa
    10141 agggcaaata cgtcgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    10201 tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    10261 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    10321 gcgtgggcga acagcagaag ttgaaagagt ttgatctcag caacccgctg gtccaggcga
    10381 agctcaaaga acgctacggt aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    10441 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    10501 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    10561 cctttccgca agctgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    10621 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    10681 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    10741 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    10801 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    10861 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    10921 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc aaacttagtc agcaatggag
    10981 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    11041 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgcgggc
    11101 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacgctaga
    11161 gagttaccga aggagaagca tctgactggt aagattttta ctcagcacat tgagcgtaac
    11221 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgt
    11281 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    11341 tggagtcatc acccttctga taagctttcc ttggatgatt accagactga atacacagcc
    11401 aaagattttt acctcattct gctctttttt ctcaaatgac atatattata ttttacatca
    11461 tattca
//
LOCUS       DAZFRJ010000139        11459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000139 DAZFRJ010000000
VERSION     DAZFRJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.140"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..791
                     /gene="nanC"
                     /locus_tag="ACHKUN_003425"
     CDS             75..791
                     /gene="nanC"
                     /locus_tag="ACHKUN_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO6661628.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            811..1917
                     /gene="nanM"
                     /locus_tag="ACHKUN_003426"
     CDS             811..1917
                     /gene="nanM"
                     /locus_tag="ACHKUN_003426"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO6661629.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1982..2962
                     /gene="nanS"
                     /locus_tag="ACHKUN_003427"
     CDS             1982..2962
                     /gene="nanS"
                     /locus_tag="ACHKUN_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6661630.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(2970..3620)
                     /locus_tag="ACHKUN_003428"
     CDS             complement(2970..3620)
                     /locus_tag="ACHKUN_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6661631.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            3757..3900
                     /locus_tag="ACHKUN_003429"
                     /pseudo
     CDS             3757..3900
                     /locus_tag="ACHKUN_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4564..4692)
                     /locus_tag="ACHKUN_003430"
                     /pseudo
     CDS             complement(4564..4692)
                     /locus_tag="ACHKUN_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4668..4817)
                     /locus_tag="ACHKUN_003431"
                     /pseudo
     CDS             complement(4668..4817)
                     /locus_tag="ACHKUN_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(4902..5099)
                     /locus_tag="ACHKUN_003432"
     CDS             complement(4902..5099)
                     /locus_tag="ACHKUN_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6661632.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(5111..5599)
                     /locus_tag="ACHKUN_003433"
     CDS             complement(5111..5599)
                     /locus_tag="ACHKUN_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6661633.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(5596..5970)
                     /locus_tag="ACHKUN_003434"
     CDS             complement(5596..5970)
                     /locus_tag="ACHKUN_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO6661634.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(6060..6428)
                     /gene="cbeA"
                     /locus_tag="ACHKUN_003435"
     CDS             complement(6060..6428)
                     /gene="cbeA"
                     /locus_tag="ACHKUN_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO6661635.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(6591..6812)
                     /gene="yeeT"
                     /locus_tag="ACHKUN_003436"
     CDS             complement(6591..6812)
                     /gene="yeeT"
                     /locus_tag="ACHKUN_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6661636.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6875..7351)
                     /gene="yeeS"
                     /locus_tag="ACHKUN_003437"
     CDS             complement(6875..7351)
                     /gene="yeeS"
                     /locus_tag="ACHKUN_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6661637.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(7367..7852)
                     /locus_tag="ACHKUN_003438"
     CDS             complement(7367..7852)
                     /locus_tag="ACHKUN_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6661638.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(7907..8725)
                     /locus_tag="ACHKUN_003439"
     CDS             complement(7907..8725)
                     /locus_tag="ACHKUN_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6661639.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRVLWVMAETLLESLR"
     gene            complement(8825..9058)
                     /locus_tag="ACHKUN_003440"
     CDS             complement(8825..9058)
                     /locus_tag="ACHKUN_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6661640.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(9137..9592)
                     /locus_tag="ACHKUN_003441"
     CDS             complement(9137..9592)
                     /locus_tag="ACHKUN_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO6661641.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(9668..>11459)
                     /locus_tag="ACHKUN_003442"
     CDS             complement(9668..>11459)
                     /locus_tag="ACHKUN_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661642.1"
                     /translation="GELREFELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKT
                     LFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHA
                     PENHKPDPEWYCTSLCRETETLCQEIYERPYNSFISDATANGLILMKLPETWSTNEKM
                     FASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAAR
                     SVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASR
                     LIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRD
                     AALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTG
                     AGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLAVASSGVAGGA
                     VGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
BASE COUNT         2922 a         2770 c         2900 g         2867 t
ORIGIN      
        1 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtttcctaca
       61 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
      121 cgtcaccatt aatttctcaa gctgcgacac tggacgtacg tggtggatat cgtagtggaa
      181 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
      241 gtatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
      301 atgatgttca ggttgatgtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc
      361 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
      421 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
      481 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
      541 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
      601 aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc
      661 tacaatacca tatgacgccg gatattacgc catacataga atatgactac cttgaccgtc
      721 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
      781 ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc
      841 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
      901 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg
      961 gcatggtaca agctggatac acaggccaaa gataaaaaat ggaaagcgtt agctgcattc
     1021 cctggtggac caagagatca agcaacctcg gcatttattg atggcaatct gtatgtgttt
     1081 ggcggcattg gcaaaaacag cgagggcttg actcaggtat ttaatgacgt acacaaatac
     1141 aaccccaaaa ccaatagttg ggttaaattg atgtcgcacg caccgatggg catggcgggt
     1201 catgtaactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     1261 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
     1321 atcaacgctc actattttga caaaaaagca gaagattatt ttttcaataa gtttctgttg
     1381 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
     1441 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     1501 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     1561 aataagcttg ctcccgtctc atcaccagat ggcgtagctg gcggttttgc ggggataagc
     1621 aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag
     1681 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     1741 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     1801 tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg
     1861 gtgacggatt cagttttgat ctctgtgaag gataataaag ttacagtaca aaactaacgc
     1921 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac gcgaaattta
     1981 tatgaacgca ataatatcgc ccgattatta ctatattctt accattgctg gtcagtctaa
     2041 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcatcccag
     2101 aatcaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa
     2161 cgacatgatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     2221 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatatagc
     2281 acggaaatta ctgcccttta ttcctgataa tgcaggggtt ctcatcgttc cgtgttgccg
     2341 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     2401 ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag tcagcagaac
     2461 gcgagccgca ctggcaaaaa atccgcataa caaattcctc ggcgtatgct ggatgcaagg
     2521 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
     2581 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     2641 accgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc atgcgtatga
     2701 agttatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     2761 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     2821 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     2881 tcatttcagc acggcagccc gtcgggggat tatttctgac aagtttgtag aagcaatttt
     2941 gcagttttgg cgcgaaaggt gagcgttcat taatttgatt tattcgcatc catcagcgct
     3001 tttaactgct ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg gcaattaggg
     3061 tacacaggca gcaaatcatt aactggatcc agctggtaat cctctttaat ggctgacagc
     3121 ggcaccaggt gatgcacatg gataaagcct tcagcgatat caccatagat cttcaccaga
     3181 tcaacaccgc atacgttgca ctggcatcca tgatgttcga ccgctttatc tcgggccttt
     3241 ttatcgcgtt catagcggtt tactgtcacc tttatcgccg caccttcaag gtatgtctcc
     3301 tgcgacggca actcatccgg gaacagaacg tcggctatgg gagccgcagc ggctttatcc
     3361 agatgaatat gaaatccctg ctctatgagt agcttgacac atttagattt tattccgccg
     3421 gtgaaatccg caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat gccagcaatc
     3481 gccttcgagg ggtaacgtcg gccatgatat tcaatctcat acaaccgcgc aggctggaac
     3541 tgatgagttt ctcccgcatc ccataaatgg atagcttcga tgatataccg ccgcggaaga
     3601 tgatcaggga gtttattcat ggagaatgtc cttatacgtt ctcagaacag gcagcaaaaa
     3661 aacaaatgag atcaaggtaa tatatcttca cttgcaggct tgaacagcag aaaatttgcg
     3721 agcatgatcg caattatttc actgccaggg atatcaatag cttccagtaa atcgctacag
     3781 caggcaattg ccaatataaa aatctggcac aagggtgaac agcgcgcgcc gcataagcca
     3841 ttgttattgc tatacgtatt agcgggatac ctgaatggac atccgcgcct tttcgattat
     3901 tgtagtggaa cagtctacga ggatgcagtt cgaagtgagt aagtaaaggt tgcgggaaac
     3961 gatcaatgcc gccatacaat gattttttag gatcaacaat gagtaataaa aactacgaaa
     4021 gtcaccgcaa agcgattgtt agtaaaggta taccacccgc tctattaaat aggctcacca
     4081 attcggatgt tcaggtgatc aataccttct taacacgagt gagcaaactg gaactatctc
     4141 aacaagagaa agactggatc ataaaaatca tctctatggt ttagacaatt tcagccttgt
     4201 agcctttcaa gggttttgtg tgggtacaaa cattatcgaa tcggggtata gccgcagctg
     4261 tagctgtatc cacatcagta agttgatgta ggttgactta ggttgagcga aaaatagcga
     4321 aaagccttgt gtggcgcgga tttcagtcat aaaaaaacgt ccgttgatgt ctattgatgt
     4381 tccgatggtg cgaaggtgcg aaggtgcgaa ggccggactc aaacatgtga cttaacctca
     4441 tgatttaaaa ttgttaataa taaacaatat catcttatac caacacagat accaacacga
     4501 aaaatcactg gttttcggca tcgaaccagg acaatcagct cctgattgag ataccgagct
     4561 ctgttactcg tcatcttcct cgacaggctt cattgcatca agtagtactc gcaccatcgc
     4621 gatagaacca atccggataa aagcatcttc tccatctata tctgttaact gatatcgctc
     4681 acttgatgcc ggataattgt gtcgatttgc agcagtgaac tctttcgtac agccagaatg
     4741 cggcggatgc tggttgagca gataacgtcc gtattctcgt tgaccacttc cggacggtag
     4801 gctgtgatat cagacataat cgatattcct ttttgcagat aaatgaaaat gccagccctg
     4861 ttcaggctgg cgtcagtgga atgaagtctg gtgagtaaga ctcaccggtt ctggtgctga
     4921 agctgcagtt ggcgcagggt acggatatcc tctctggcct gttcgataca gggcaacagt
     4981 gctgctgaat ccgttttatc ctcattgttg tcaaagataa ttcccgattc gcagtcgata
     5041 ttgtcctgca gccacgcaat cagaatatcc agtgcggttt ccgtggttaa tgatttcatg
     5101 ttgttcctgg ttaccagttg aaagtgggta aacccggcag gcattcagcg ctggcatcca
     5161 gatgaatgag actgacgcca taacgtcggg taagtgtgat aaccagacga cggaacgttt
     5221 ttgacagccc caggcgttta agccgcagaa ccggatatga ccacgcatcg gtacgtaaca
     5281 gatatccgct acctgtgaaa tgaatccatt ctgattcacc aacgtcactg gtctgatgcg
     5341 acagcgagta cagccaggcg ttatcctttt ccgtgatatg tgcggtactg cagcgtatgc
     5401 cggcaagggt tgcaaacggt ggtagtgtgc aggttgactg ttggtcggat tcctccacca
     5461 cgcggagtga ataaccgttt tcagcgacct tgttaatcag ttcagtgaga ttaacaccat
     5521 cgacgtcaac gacaattcgc cccatgttca gtgcctgcac attaattctg tcggcttcca
     5581 gcatcaggga aagtttcatc gtttctccgg atgcttaccc agggtaatgt tatttaccgt
     5641 tctgtaattg tcgcgggtca tcaggccggt cgccctgcga gcccggagga tatcaatact
     5701 gtttattaac tgagagcggg gacaagtgct gaatcccggc tggtcggtac gcaccagcgc
     5761 gtatttttcc acgagaaaat tcaccgcatc acacagtgaa atgccagctt caatatgctg
     5821 ctcaatcaca cgttcatcgg cgaacggtgt gtcattcagt gtaaggccgt aatgctggcc
     5881 cagtagtcgg gagagcagag tctgccagat ggtgacggga gacgggcagc tcgatgcctc
     5941 ccgtacgtgt gtatcgggta atgttttcat actgaaggtt ttcctgatat gcagagatga
     6001 aaaatggaaa agtggcgtgg tgaaagcacc aggccggagc agaaggttat tccggtggtt
     6061 tactttttcg tttcgggcgt cggataaaca gccagataaa cgtaaccacc gctaccgaga
     6121 gtgtcagctt tgcaggtcag accttttgca tacagcgtga cggtatgctg atggcgggga
     6181 ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg aaaggcctgg
     6241 tccaggtggt acgcatctgc atcgctgaac aggcctctga taccggcgcg gtcggcaagg
     6301 taatgcaggc ggttaccctc ctgcaccaga cgggcgccga aacagggcgt cactgtgcag
     6361 ggtagtcccc accagggatg gtcgtcattg tcatcgggat gcgttgtccc ggaaagtgtg
     6421 tctgacacga taaaatcctc acagaaaatc ggtgaatgat agtttaacga tgacgggtca
     6481 gccggcgcgc agaccggctt cggtaaaggt gtattcgttg atgagcagga cctcatccac
     6541 agcggcgtct gagctcagcc agtcgtactc gttttccggg tgacggaaca tcagttcagg
     6601 gtgacgctca tcagccggac atacggacca aaactgtcct tacggcgttc tgcaaacacg
     6661 gccagcacac cgggaatatc ctgcacttca cgaccggtat acgcttcagc actgccgtgc
     6721 cagcggtatt tgccggtaca gaacggaaaa agacgggatg ccggatgctg acggtgaata
     6781 cgcatggctt cgccacgggt gatgattttc atagtgggat acctctgaag acagaagata
     6841 aaagtgaaaa caggtgtgat gtggttgtga cgggttaaag cagaccatgt tcggcaaagg
     6901 agaaaacctg gctgccaccg actatcagat ggtccggcac ccggatatcc accaggccca
     6961 gtgcctgtac cagacgttcc gtgataaggc ggtctgcctt gctgggtgtg acttcaccgg
     7021 acgggtgatt gtgtgccagc accacagcag cggcattgtg atacagggcg cgtttaatca
     7081 cttcacgggg atggacttcc gtgcggttga tggtgccggt gaagagggtt tcaccggcaa
     7141 tcagctgatt ctggttgttc agatacagca cccggaactc ttcacgctcc agtcccgcca
     7201 tgttcagaat cagccattct cgtgccgcac gggtggaggt gaaggccacg ccgggttcat
     7261 gaagatggcg gtccagggtt ttcagggccc gttgaatgag gctgcgctcg ccgggcgtca
     7321 tctctccggg cagaaaggaa agctgttgca ttgtacttct ctccattcag tcgatgatgc
     7381 gcataatggc gctgcattcc ggatgctgca gggcgtaatc ccgcagccgg taataatgga
     7441 ccgtcatggc ataacactcc gtacgacagg catgatgact gtacgtcatc agacaggcag
     7501 caatgccggc agcttccgga ctcatttcag cgcggttacc gttcatggca ttgaacagta
     7561 cccatgtttc gtcatcatcg tcatccggtt cgggtgccat aaatgccccg ccgttgttca
     7621 gggtgtacag attccagata ccaccgcagt agtcttcaca cagacggtcc atccagccga
     7681 agacacgggg ctccagggtc acccactgtg gaatgaggcc aaagtgctgc ggccagaagc
     7741 tgatgcgctg ttcatcaggg acgagggtgg caaccaactg aggctggtca ttccctgatg
     7801 cagcggttac ggaaacagaa gatgtggtgg aattatgtga aatggttttc atgaaatgac
     7861 tccttaaagg acaaataaat gcgtggtttt cagggtaatg gctgaatcag cgcaggctct
     7921 ccagcagggt ttccgccatc acccacaata cgcggttgag cttaatgtca gtgtcaatgc
     7981 tgtgaatggc acgggtatgg atacgttttc ctttagcgct gcgacctgaa attccgcctt
     8041 tcagcatatt ctcctgaatg gtctgataag cactccacag gtccttaccg taatcctccc
     8101 ggcgtcgtgg cgtcagaatg tcggcggtgg tgacgggctg atgttcgtca ccataacggt
     8161 aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg cggcagaatc agcgactgca
     8221 tggcatcacg cttttcctca atccggtcaa acacgcccac cacctcgtaa gccccttcaa
     8281 tgactctgtc caccacattt ccccggtgcg gaacacgcac ttcccccaga gactgaccac
     8341 agacgcatcc gttctggcag acgaacctga agtaacccgg cagcatctgg tagctggagg
     8401 taccgtcatg agagttgagc agaataattt cagggacatg ttctccgttt atctctccgg
     8461 cccgccgcag acgcagcatg tgttttgtgt atccccggcg gcccgggtcg cgcacacggg
     8521 tctggcaggc gaagaatggc tgaaagcctt cccgctgcag gctttccagt acggtgatgg
     8581 tggggatgta cgtatagttc tggcttcggg acgtgtgttt atcttccccg aaaatccccg
     8641 gcacatagtg catcagttct tcgtgtgtca gcggacggtc acggcgtatc tggttcgcat
     8701 aaccaaaacg actggctagt cgcataattt gctccttatc ggtggttaag atttactggt
     8761 gtaataaatg aaaaagccac gtctcccgga gaagacgcgg cctgatgggt gaaatgaatg
     8821 acgtttattg tctgagaaga ccgtcactgg cgagccgcgt attaagcacc tttccggcat
     8881 cgggttcaga attccggcag cgccagcgtc gctgaccttc cgcgttgcgg ataacccgac
     8941 ggaaatgcat tcccgggtca tcctggataa acacattgcg gtaagcggtt atgacagcaa
     9001 ccgcctgccc gtatgagaaa gatccttcag ccaggacata ctctgtgtaa cccggcatat
     9061 ctgttttctc ctgataaata aaaagcctct gccacgcgtt gtgacagagg ctgaagcatg
     9121 aaactgactt cagggatcag ttaacgtttt ttccggaaac ggtaatcagc agtggatggt
     9181 agtcctgggg attgaaaacc gataacggca gactgacacg atggccgtta ctttcttcag
     9241 ttgctttgat gatttcggtt gtggcgacat tttccacgca ctccgtttcc agaaatgcgt
     9301 ctgtggttcg cgtggcatta ctgtcaccaa aagcttccgt ttccattttt ctggtcacca
     9361 gcgtctgacc atatttgtct ttgagttgca gagtgatggt gagggggcca aatccttcat
     9421 cgtttccgcc attatccagc cggaactggt aagcacaaat atttcccggg agccatatcg
     9481 tatctgtatt gcgtatactg atgtaacgtt gatcctgtgc ccggagtggg gcagaccacg
     9541 ttaaccccag aatgaaggcg gtaatcatgc aggttttgaa caggtgaatc atggtattta
     9601 cctctctgag tcatgacgat gacactgaca aatcaggtga taaagcgtaa aaggcgcaga
     9661 atagccgtta tgccggtaac tccgggggta atgcttcttc cagtcggtta accatattgc
     9721 cgagatggga tgcatcatat tccatgacgg ggcgttgcct gatgatactg accaccagtg
     9781 gtttgattaa catattggtc gcggcccgtt gttgtatacc ggcggcgaaa atgatctcca
     9841 gagcattctg atcgataaca cgggccagat tctcgttcag attttcaatc tcatgtccgg
     9901 tcagcaggaa actgccggca agccatgtca cctgctcctg aatcattgcc agaattttga
     9961 cgcgatcttc ttcaaccagc gctccggcaa tttttcctga tacagcggag gcaatcattc
    10021 cccccagaac accgccaacc acacgccctg tcagtgcacc aaatggtccc agtggactga
    10081 acagtattcc gcccgccact gacccgacag caccacctgc cacaccggaa gatgccacgg
    10141 caagattcct gatgaactgc gtaccggaga tccgtccccg caacattttg atcatgtcag
    10201 ggccggttgt gacagctacc agtgccagag atgtcaccag cgtacccttc atcactttgt
    10261 tcagggcact gatatttcct gcaccggtcc ccttctgaag agccagtcga acagtcgggc
    10321 tggcagtcga aaaatcaatg tgcttcagca taccctgaac aacactgagc cggtggagtt
    10381 gctgggttgt gacgtagaca gccagtgtgc gggtgaaggt ttttcctgcc tggacagcag
    10441 ctgtctgcag ggcagcatcg cgatcgccgg tgctgagcca gaagaccgat gccgtcaggg
    10501 caaaactgat ccctccggcc gccagactga caaccgaacc ctcggcaata tcataagtga
    10561 ccgattcgat ggtcccgaac cgggtgatat tacgggcctg agtataagtc agatgtcccc
    10621 gacgaatcag ccgggatgct tctgcgggat cggttacacc gggtacttta ccttcgcgga
    10681 tcttattcct catggtttca acagcgccgg catactgatc acgggggact tccagttgca
    10741 tggggccatt atttcccatg taacgatact gtccgttttg tccgtcgaat gccgcaccga
    10801 cgctacgggc tgcagttgaa caatatttcg tctggatttc tgttccgctg accagtctgt
    10861 ctgctccatt tctggcatta ttatcaccga ggatccgtgc gtttttcaga cggactctgt
    10921 cgacaatatg gtttccccgt tcagcggcaa acccatgtcc ctgccctccg gaagcaaaca
    10981 ttttctcatt tgtactccag gtttccggca gtttcatgag aatcagacca ttcgccgttg
    11041 catcggaaat aaagctgttg taagggcgtt cataaatttc ctgacacagt gtttctgttt
    11101 cgcgacaaag agaggtgcag taccattccg gatccggctt atgattttcc ggggcatgat
    11161 gtacttctgt cagcccgcag gaggcacaga tacgaagctc atccacgtga tggaatgata
    11221 caaaacggcg accttctgaa aagggatacc agtcaccgcc gtccaggcaa cgatcgagaa
    11281 aatcgtcatt cgcttccggt gataaattca ggcggttccg gaagagcgtt ttcagttcat
    11341 caggtgtgaa tgacaggttc tctttcaact gttcgttaaa ccctgccatg tttctttcat
    11401 accaggcatt gttcagtaac cagtttccgt ctgccagttc aaattccctc agctctccc
//
LOCUS       DAZFRJ010000140        11432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000140 DAZFRJ010000000
VERSION     DAZFRJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.141"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            267..1133
                     /gene="folD"
                     /locus_tag="ACHKUN_003443"
     CDS             267..1133
                     /gene="folD"
                     /locus_tag="ACHKUN_003443"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO6661643.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1135..1347
                     /gene="ybcJ"
                     /locus_tag="ACHKUN_003444"
     CDS             1135..1347
                     /gene="ybcJ"
                     /locus_tag="ACHKUN_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO6661644.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVEVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1455..1976
                     /locus_tag="ACHKUN_003445"
     CDS             1455..1976
                     /locus_tag="ACHKUN_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6661645.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2012..3397)
                     /gene="cysS"
                     /locus_tag="ACHKUN_003446"
     CDS             complement(2012..3397)
                     /gene="cysS"
                     /locus_tag="ACHKUN_003446"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO6661646.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3571..4065
                     /gene="ppiB"
                     /locus_tag="ACHKUN_003447"
     CDS             3571..4065
                     /gene="ppiB"
                     /locus_tag="ACHKUN_003447"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO6661647.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4068..4790
                     /gene="lpxH"
                     /locus_tag="ACHKUN_003448"
     CDS             4068..4790
                     /gene="lpxH"
                     /locus_tag="ACHKUN_003448"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO6661648.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4908..5417
                     /gene="purE"
                     /locus_tag="ACHKUN_003449"
     CDS             4908..5417
                     /gene="purE"
                     /locus_tag="ACHKUN_003449"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO6661649.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5414..6481
                     /gene="purK"
                     /locus_tag="ACHKUN_003450"
     CDS             5414..6481
                     /gene="purK"
                     /locus_tag="ACHKUN_003450"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO6661650.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6618..7511)
                     /locus_tag="ACHKUN_003451"
     CDS             complement(6618..7511)
                     /locus_tag="ACHKUN_003451"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6661651.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7508..8323)
                     /locus_tag="ACHKUN_003452"
     CDS             complement(7508..8323)
                     /locus_tag="ACHKUN_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6661652.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8334..9593)
                     /locus_tag="ACHKUN_003453"
     CDS             complement(8334..9593)
                     /locus_tag="ACHKUN_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6661653.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9603..11270)
                     /locus_tag="ACHKUN_003454"
     CDS             complement(9603..11270)
                     /locus_tag="ACHKUN_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6661654.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2543 a         3252 c         3025 g         2612 t
ORIGIN      
        1 aggctgaaaa ttctttttat attgtcaggt atttcttaaa ttatcttaat ccttaaacaa
       61 ggaaataaat cagttccaga tttacaacgc catcatggac gaaaaatgaa gctttcaatc
      121 tcagcgacct cagcgacggt gcgcctcacc ttcgcaagag gtcgcttcac gcgataaatc
      181 tgaaacgaaa cctgacagcg cgccccgctt ctgacaaaat aggcgcatcc ccttcgacct
      241 acgtaacaga tggaatcctc tctctgatgg cagcaaagat tattgacggt aaaacgattg
      301 cgcagcaggt gcgctctgaa gttgctcaaa aagttcaggc gcgtattgca gccggactgc
      361 gggcaccagg actggccgtt gtgctggtgg gtagtaaccc tgcatcgcaa atttatgtcg
      421 caagcaaacg caaggcttgt gaagaagtcg ggttcgtctc ccgctcttat gacctcccgg
      481 aaaccaccag cgaagcggag ctgttggagc ttatcgatgc gctgaatgcc gacaacacca
      541 tcgatggcat tctggttcaa ctgccgttac cggcgggtat tgataacgtc aaagtgctgg
      601 aacgtattca tccggacaaa gacgtggacg gtttccatcc ttataacgtc ggtcgtctgt
      661 gccagcgcgc gccgcgtctg cgtccctgca ccccgcgcgg tatcgtcacg ctgcttgagc
      721 gttacaacat tgataccttc ggcctcaacg ccgtggtgat tggcgcatcg aatatcgtcg
      781 gtcgtccaat gagcatggaa ctgttgctcg caggttgcac cactaccgtg actcaccgtt
      841 tcactaaaaa tctgcgtcac cacgtagaaa atgccgatct gttgatcgtt gccgtcggca
      901 agccgggctt tattcctggt gactggatca aagaaggcgc aattgtgatt gatgtcggca
      961 tcaaccgtct ggaaaatggc aaagttgtgg gcgacgtcgt gtttgaagac gcggctaaac
     1021 gcgcctcata cattacgcct gttccgggcg gcgttggccc gatgacggtt gccacgctga
     1081 ttgaaaacac gctacaggcg tgcgttgaat atcatgatcc acaggatgag taacatggcg
     1141 acattttctt taggtaaaca tccgcacgtt gagctgtgcg acttgctgaa actggaaggc
     1201 tggagcgaaa gcggcgcgca ggcgaaaatc gcgattgccg aaggccaggt ggaagtcgac
     1261 ggtgcggttg aaacgcgcaa acgctgcaaa atcgtcgccg gtcagacagt gagttttgca
     1321 ggtcacagcg tacaggttgt tgcctgatcc ctttcccggc ctgtatcaac aggccggttc
     1381 tcttttcaga cttttgccct ctacgccccg ttcatccatg ctgatagcat cgacattttg
     1441 ccgaagcgac catcatgcca accgttatta cgcacgccgc cgttccccta tgcattggtt
     1501 tagggttggg ttcgaaagtc atccctccgc gtctgttatt tgccggaatc atcctggcga
     1561 tgctgcccga cgccgacgta ttgtcgttta aatttggcgt tgcttacggc aatgtttttg
     1621 gtcatcgcga gtttacccat tcgctggtgt ttgcgtttgt tgtcccgctg ttatgtgtgc
     1681 tgattggacg ccgatggttc agggctgggc tgattcgctg ctggctgttt ttaaccgtct
     1741 cgttgctatc gcacagcttg ctggattcgg taaccactgg cggtaagggc gttggctggc
     1801 tgtggccgtg gtcagatgaa cgctttttcg ctccctggca ggtgattaaa gtcgcgccgt
     1861 ttgcattgtc tcgttacacc acgccgtacg ggcatcaggt gattatttct gaattgatgt
     1921 gggtgtggtt gccggggatg gtgcttatgg gaatgttgtg gtggcgcagg cgataaccgg
     1981 atgcgaaaac tcgcatccgg caacagcgca attacttacg acgccaggtg gtcccttgcg
     2041 ggccatcttc cagcacgatc cccatctcgt taagacggtc acgcgccgca tctgccgccg
     2101 cccagtcttt cgctttacgg gcatccagac gctgttgaat taacgcttca atctcagcca
     2161 cttcgctgtc gtctgcctgc gcaccgcttt gcaggaacgc ttccggttct tgctccagca
     2221 ggcccaatac agcggaaagt ttacgcaggt gagatgccat tgcattcgcc gctgccatat
     2281 cttctgcttt cagacggttc acttcacgcg ccatatcaaa cagcacggaa taggcttccg
     2341 gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc ttcaaacgct tcgccaccgg
     2401 caggcgcaac ggttttatct gtgccgcgca gcgcaatgta gaggcgctcc agcgccgcac
     2461 gcgcctgctt caggttctct tcgctgtagt tcagttggct gcgatagtgg cccgacatca
     2521 ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac atcgcgcacg gtaaagaagt
     2581 tacccagcga tttggacatc ttctcgcggt caaccatcac catccccgag tgcatccagt
     2641 agttcacata ctggccatca tgggcacagg tggactgcgc gatttcgttc tcgtggtgcg
     2701 ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg gttacccagc tgcttgcagt
     2761 tcattgccga acattcaatg tgccagcctg gacggcccgc gccccacgga gacggccagc
     2821 tcggttcgcc ctctttcgac atcttccaca gaacgaagtc catcgggttg cgtttgtcgt
     2881 cgaccacgtc aacgcgcgca cctgcctgca gctggtcgag atcctgacgc gacagcacgc
     2941 catatgtcgg atcggtcggg acgtcgaaca tcacgtcgcc gttgtccgcc acataagcgt
     3001 gacctttggc gatcagttgt tcagtgagtt caataatttc tgcgatatgg tgcgtcgcgc
     3061 gcggctccat atccgggcgc agaatgttca aagcatcaaa atctttgtgc atttcggcga
     3121 tcatgcgatc caccagcgcc acaaagcttt cgccattttc attggcgcgt ttgatgattt
     3181 tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt atagccgagg aaacgcagat
     3241 agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg accgatatga cagagatcgt
     3301 aaacggtgat tccacacaca tacatgccga cttccccggc gtgaataggc ttaaattcct
     3361 ctttttggcg tgtcagagta ttgaagattt ttagcatcga agattccgtt tagacatgtg
     3421 tgggtaattg agttgcgtat aatacccata tttcccgcgc gaatcagcat acattgcgtg
     3481 atgatccgat cgtgcggtta tgctataaca ccaccctata tatgacccga actgggttga
     3541 agcaccaatc aaacggaaca ggatgcaaaa atggttactt tccacaccaa tcacggcgat
     3601 attgtcatca aaacttttga cgataaagca cctgaaacag ttaaaaactt cctggactac
     3661 tgccgcgaag gtttttacaa caacaccatt ttccaccgtg ttatcaacgg ctttatgatt
     3721 cagggcggcg gttttgaacc gggcatgaaa caaaaagcca ccaaagaacc gatcaaaaac
     3781 gaagccaaca acggtctgaa aaatacccgt ggtacgctgg caatggcccg tactcaggct
     3841 ccgcactctg caactgcaca gttcttcatc aacgtggttg ataacgactt cctgaacttc
     3901 tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg ctgaagtggt tgaaggcatg
     3961 gacgtggtag acaaaatcaa aggtgttgca accggtcgta gcggtatgca ccaggacgtg
     4021 ccaaaagaag acgttatcat tgaaagcgtg accgttagcg agtaatcgtg gcgacactct
     4081 ttattgcaga tcttcatctc tgcgtggaag aaccggcgat caccgccggt tttctgcgtt
     4141 ttttagcggg ggaagcccgc aaggccgacg cgctgtatat tcttggcgat ctgtttgaag
     4201 catggattgg cgacgacgat cccaacccac tccatcgcca gatggcggca gcgatcaaag
     4261 cggtgtccga ttccggcgtt ccctgttatt tcattcatgg caaccgtgat tttctgctcg
     4321 gcaaacgctt tgcccgtgaa agcggcatga cattattgcc ggaagaaaag gtgctcgaac
     4381 tttatggtcg ccgggtgttg attatgcatg gcgacacgct gtgcaccgat gacgcgggtt
     4441 atcaggcttt tcgcgccaaa gtccacaaac cctggctgca aatgctattc ctcgccctgc
     4501 cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgagc gaacagtaaa gaagccaaca
     4561 gcagtaaatc gctggcgatc atggacgtta accaaaacgc ggtagtcagt gcgatggaaa
     4621 aacatcaggt gcaatggctg atccacggac atacccatcg cccggcggta catgaactta
     4681 tcgccaacca gcaacctgct tttcgcgtgg tactcggtgc ctggcatacg gaaggttcga
     4741 tggtgaaagt cacggcggat gacgttgagc tgattcattt tccgttttaa aaaacccgca
     4801 actttgctga tttcacagcc acgcaaccgt tttccttgct ctctttccgt gctattctct
     4861 gtgccctcta aagccgagag ttgtgcacca caggagtttt aagacgcatg tcttcccgca
     4921 ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag cgactgggct accatgcagt
     4981 tcgccgccga aatcttcgaa atcctgaatg tcccgcacca cgttgaagtg gtttctgctc
     5041 accgcacccc cgataagctg ttcagcttcg ccgaaagcgc cgaagagaac ggttatcagg
     5101 tgattattgc gggcgcaggc ggcgcagcgc acctgcctgg tatgattgcc gccaaaacgc
     5161 tggtgccagt gctgggcgtg ccagtacaga gcgccgcact gagcggtgtc gatagcctct
     5221 actccatcgt acaaatgccg cgcggcattc cggtgggtac gctggcgatt ggtaaagctg
     5281 gcgcggcaaa cgcggcgtta ctggcagcac aaattcttgc gactcatgat aaagaactgc
     5341 accagcgtct gaatgactgg cgcaaagccc agaccgacga agtgctggaa aacccggacc
     5401 cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac gggcagttag gccgtatgct
     5461 gcgtcaggca ggtgaaccgt taggcattgc tgtctggccg gtcgggctgg acgctgaacc
     5521 ggcggcggtg ccttttcaac aaagcgtgat taccgctgag atcgaacgct ggccggaaac
     5581 cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg aaccgcgatg tgttcccgat
     5641 tattgccgac cgtctgactc agaagcagct tttcgataag ctccacctgc cgaccgcacc
     5701 gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg tttgagcgtt taggtgaact
     5761 ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc ggtcaatggc gtttacgtgc
     5821 caatgaaacc gaacagttac cggcagagtg ttacggcgaa tgtattgtcg agcagggcat
     5881 taacttctct ggtgaagtgt cgctggttgg cgcacgcgga tttgatggca gcaccgtgtt
     5941 ttatccgctg acgcataacc tgcatcagga cggtattttg cgcaccagcg tcgcttttcc
     6001 gcaggccaat gcgcagcagc aagcgcaagc cgaagagatg ctgtcggcga ttatgcagga
     6061 gctgggctat gtgggcgtga tggcgatgga gtgttttgtc accccgcaag gtctgctgat
     6121 caacgaactg gcaccgcgtg tgcataacag cggtcactgg acacaaaacg gtgccagcat
     6181 cagccagttt gagctgcatc tgcgggcgat taccgatctg ccgttaccgc aaccggtagt
     6241 gaatagtccg tcggtgatga tcaacctgat tggtagcgat gtgaattatg actggctgaa
     6301 actgccgctg gtgcatctgc actggtacga caaagaagtc cgtccggggc gtaaagtggg
     6361 gcatctgaat ttgaccgaca gcgacacatc gcgtctgacc gcgacgctgg aagccttgat
     6421 cccgctgctg ccgccggagt atgccagcgg cgtgatgtgg gcgcagagta agttcagtta
     6481 agcaggtgct catttgattg ccggatgcgg cgtaaacgcc ttatccggct taccgctccg
     6541 gtacccattg taggcctgat aagacgcgtc aagcgtcgca tcaggcatgg tgcaccgatt
     6601 gccggatgct ctcatcacta cagcgaaata caggtccccg cttcgcccgc cagcgtctct
     6661 tcaattcgcg ataacgcccc aatccacgcg ggtttaccac ggcttctgac ataaccactt
     6721 accgccgtta ccttcggccc catcgaacca tcggctttgg taaatggcgc taactcatcc
     6781 ggtgtggcat ggcgaatggc gcgttgctgc ggcgttcccc agttttcata aaccgcgtca
     6841 gcatcggtga ggatcaccag tccatcggca ttaatctgct cggcgagcaa cgcagcggcg
     6901 agatctttat caatcaccgc ttcactccct gccccatctt ccgtcacagg cacaccgccg
     6961 ccgccactgc aaatcaccac atgcccctct ttgagcaaca actcgatggc ttcgctgtcg
     7021 agaattttac gcggttgcgg agacgccacc acccggcgca aatatttacc atcacgtttc
     7081 atctgccagc cgtaagccgc ttccagtgcc ttttgttctt ctggctgata aaccggacca
     7141 ataaatttct ctggctgcaa aaacgccgga tcatcaggcg aaacctcaat gcgcgtcagc
     7201 accgtcgtga caggcggcat ctgcggctgt gcgctcaaac tctgcgccag catatagcca
     7261 atcatcccct ggctttccgc caccagcaca tccagcggat acggatctac ctctttccac
     7321 gccagattct gcaatgccag cagccccacc tgcggcccat tgccgtgaac aatcgccagc
     7381 cgataagaac gggccaggcg cgccagcgcg ggtacagcac tggcgatatt gcgatattga
     7441 ttttctgccg tcagcgcctc accgcgctgg agtaaggcgt tgcccccaag agctacaacc
     7501 agtgttttca tggttttcct tgtaataatt gttggccaag ccagaacccc agcagcgtat
     7561 ctgcccctga cgtatgcccc agcgccagca gcgaatcaat cgcaacagcg gtacgcttcg
     7621 ggcaactcag agcatggaca aagtgcagga gtggcgaggc gaaatatcct tgcgcggcat
     7681 aacgtaaata actgacgctc accgctgtgg taacgagttg aagattgtcg gaacaggcaa
     7741 aaaacgggcg accggcgcgc gcatctaaag cgccataata ccaggccgcc agcagcattc
     7801 cgctcaacgt gtcatcatga cttggcgtta atccggggcc tttacccagc cagtgccgcc
     7861 agtcggtctt aacgccattg agcgcggcct gaaaacagtg acgaaactgg cgtaattcag
     7921 cgggcagcgg atcgcttgcc gccatcgcca gtggtccgaa aagtccggtt tcctccgcgc
     7981 gttgcatcca tgcagctgca agcggttgag gatgcgaagg cggcgtaata cgcagcaaac
     8041 aataacgctg tggctgttta accgtgaaac gcccgaggcg aatcccttga gccacaacct
     8101 gtggtcgttc attgccgcat aatccaccgc ataacgcatc gaattgcgcg cggcgaagca
     8161 cccatcctcc ggggccgaaa ccactaccct gacgatgcag cgttaacagt tcgccgcttt
     8221 ccgtcatcag gttaatcgcc cgtcgccaca ctcccgctaa ccgccaggac tggcgataat
     8281 tcggtgcact acggctggca agcagaggat ggatgatcgt cattgcgcgt tcctcaacca
     8341 atccccatgc tttcagccag tgcttccagc gcctgttcaa aacacgccag cggtgcccgc
     8401 acagtgcctg cgccaatctg cccgatcccc gcctctttat gggcgatacc ggtattgatg
     8461 agtggcgtaa tgccggtttc taccacgcga cgaatatcca gccccaggca cgcgccctgg
     8521 aaatcccagc cggggatctg caactgcata ttgcgctcaa ggtaaatttc cgccatctct
     8581 tcagataccg ctctggccgc ttccatgcca cccgcaccga caaagcgcgt tacgccaggc
     8641 gctgcgatca ttgccgcacc tccgatacca aaggtttcgg taatcgcgct atcgcccata
     8701 tccgggttcg cctgctcctg cgagaagccg gtgaaaaaca gaccttgcgg agtgtttaca
     8761 ggcgcagtaa accagcgttc gcccagcccg cttacccgaa tcccgaacat attgccgttg
     8821 cgggtcattg ccgtgacgat gctgcctgcg cggatcatcg cgccagcatc catcgccgcc
     8881 ttgcagtaag ccatcgcgag gttgaggaag aactgatcgg tcacgctgag gaaatccatc
     8941 acttcggcga tgtgctgttt atcatgatcg aggcgagcaa tttgtggggc cagcgcacgc
     9001 attaacagtg cagaggaagc aatattgcgt tgatggaact catcgcccat cgtaatgccc
     9061 tgcgccatca tcgccgtgag atcgataccg cgctccatgc gccccagcgc cgcgcttaat
     9121 actggcatta acacatcgcg catccagcga tggcgagtca ggacatcttc gccgtaagcg
     9181 ccaaaacgca tcactttgcc gataccttcg ttgaggttgc agtacgcccg gttgccgtcg
     9241 gtcacgttct cgaccaccag catcggcata ctggcagaag taataccgcc cattggaccg
     9301 acggcattca catggtgaca aggaatgaag ttcacttccc cctgctccag tattgccagc
     9361 gcctgcgctt catctttcgc ccagccttcg aacagacatg cgcccacgca cgcccctttc
     9421 atgggtccgg tcatctcctg ccagcgcatt ggcggcccgg cgtgaagcag tgttttgccc
     9481 tcgtttagtt cgctgataag tgaagaagcc ggttgcacat ccagccagtg tggacgagca
     9541 cgacgaattt gttcgataac cgcagcattg gcttgcgcca ctgatgtaaa catgggaacc
     9601 ccttattgca aacgttctaa taaacgagcc agttttttat tgccgccggc gactggcgac
     9661 cattggtaat gcacaaccgg tttgctggcg ctttgtagct ccagcgcaaa gctgcgtaat
     9721 ccgatgttaa tcacggcgac gttttccagt aatgacggtg tgtgttgctg tgtggcagac
     9781 gagagcggat gaattaacgc tgccgccagc aaggtggctt ccggtagcga actcactacc
     9841 gcgatccccg catcttccag cgtggcgatt tgctgcgagc ggcattgcgg gtcacgttcg
     9901 gtacctgtca ccgtggcaat ggcgtacagc ggttggctgt ctgaacgcgc ggcacaggct
     9961 ttttgccagg cgctcaccag cgaggcagca ggatcggcgg tcgcaccgaa gccaatcacg
    10021 acatcaagca gcaacacgcg cacttgcggt ttagcgccga gatcggcaat taactggttg
    10081 cgtaaggctg ggtcgatcat cggatgggga cgcccgacgg tgtagaaatc atcgcccaga
    10141 tcaatgatct ggtgaccatc ggcgtccagc atcatgccgt gatgatgggt atcgtcggct
    10201 tccacgccaa ggtgcccggc aagtaatccc gccgcttcgg cagcaagcgt accgccggta
    10261 tacaaaccgc aaataaatcc gctgctgaca ggcgctattg cgttacgtcg cgccgtgacg
    10321 cgtgaaagca ggcaagccag gcgtgcggcc tcatccagcg aggaggcaaa ccagacattc
    10381 tcgtcgcggg ccaccgccgg ggtataacct aaaaacagcg ccaccgtcgg tttgccggtt
    10441 gctttcatgg cattaacaat tttcagacgc acagcttcgg caggtggttt tgaaacaaat
    10501 gccagcactt cgcttttctc gtctgcactg agcatttcca gcgctgttag cgcactgatg
    10561 ccgcccactt cacggctgag gtcgcgcccg ccaaggccaa tcgcgtgagt aattccctcc
    10621 cctgccagcg caatctgcga acacagctcc tgaatcccgg taccggaagc gccaatgacg
    10681 ccaatattgc cttctggcat cacgttagca aaagccagcg gtgtgccggc aatcatcgac
    10741 gtaccgcagt ccggccccat caccagcaag cctttttccc gcgcgcgggt tttaagttgg
    10801 atttcatctt ccagcgtgac gttatcggag aacatcatca cgttgaggtt gcgatccagc
    10861 gcctggtttg tcagctccgc cgcatactcg ccagccactg aaatcagcgc cagattggca
    10921 tcgggtaatt tctgacaggc gctgtcccag cgacgcacct gcgtcaacgc ctggctgctg
    10981 cctgacccct gcgccagttg ttttagcgcc tcttcaagct gctgcataat cgcctgcgcg
    11041 atccccgcat ccgccgcttc gctacgaatt gccacgcaaa tatcgttcgg cgtggcgtta
    11101 ttaaaatcgt catgccagaa acctgtggta tctaataacg ctttattggc gggcgtaccc
    11161 atcattacgg aaacatcatc aacattttct gattcgctga gttttcgtga aataatcatt
    11221 aaactgaccg aatcctgaaa acaccctttt ttaataaagg cgtggatcat aacaactcct
    11281 taaaaattgc acccatggca attgcacgag cagtgttaat gtggcgatta cggtatcgat
    11341 gacaacgagt aaataaggtg atgaatatca cacagggatt attaacaaaa gaattcaaat
    11401 aaccgccagg ctattacgca ttatttttta at
//
LOCUS       DAZFRJ010000141        11393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000141 DAZFRJ010000000
VERSION     DAZFRJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.142"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(124..897)
                     /gene="uxuR"
                     /locus_tag="ACHKUN_003455"
     CDS             complement(124..897)
                     /gene="uxuR"
                     /locus_tag="ACHKUN_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO6661655.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(1112..2572)
                     /gene="uxuB"
                     /locus_tag="ACHKUN_003456"
     CDS             complement(1112..2572)
                     /gene="uxuB"
                     /locus_tag="ACHKUN_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO6661656.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2653..3837)
                     /gene="uxuA"
                     /locus_tag="ACHKUN_003457"
     CDS             complement(2653..3837)
                     /gene="uxuA"
                     /locus_tag="ACHKUN_003457"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO6661657.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4289..4381
                     /locus_tag="ACHKUN_003458"
                     /pseudo
     CDS             4289..4381
                     /locus_tag="ACHKUN_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4624..5526)
                     /gene="fimH"
                     /locus_tag="ACHKUN_003459"
     CDS             complement(4624..5526)
                     /gene="fimH"
                     /locus_tag="ACHKUN_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6661658.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(5546..6049)
                     /gene="fimG"
                     /locus_tag="ACHKUN_003460"
     CDS             complement(5546..6049)
                     /gene="fimG"
                     /locus_tag="ACHKUN_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO6661659.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(6062..6592)
                     /gene="fimF"
                     /locus_tag="ACHKUN_003461"
     CDS             complement(6062..6592)
                     /gene="fimF"
                     /locus_tag="ACHKUN_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO6661660.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6602..9238)
                     /gene="fimD"
                     /locus_tag="ACHKUN_003462"
     CDS             complement(6602..9238)
                     /gene="fimD"
                     /locus_tag="ACHKUN_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO6661661.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(9305..10030)
                     /gene="fimC"
                     /locus_tag="ACHKUN_003463"
     CDS             complement(9305..10030)
                     /gene="fimC"
                     /locus_tag="ACHKUN_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6661662.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10067..10606)
                     /gene="fimI"
                     /locus_tag="ACHKUN_003464"
     CDS             complement(10067..10606)
                     /gene="fimI"
                     /locus_tag="ACHKUN_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6661663.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(10671..11219)
                     /gene="fimA"
                     /locus_tag="ACHKUN_003465"
     CDS             complement(10671..11219)
                     /gene="fimA"
                     /locus_tag="ACHKUN_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6661664.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         2745 a         3031 c         2742 g         2875 t
ORIGIN      
        1 tactaatatt caggttatac agatatttaa acaataccct gcaatttaca aagcaatact
       61 cattgcgact tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat
      121 aagtcagacg tcgactttat ccagcggcca tgaatcaaac agatagccgt caaaataaat
      181 atcgtcaacg ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat
      241 tgccagcttc gccgctcggg catctttttt gattaacgcg gcgaggatct gtttgtgatc
      301 gcccaaccac tctttgcgat acaggctgtc atccagatgg ctgtgcaact ggatccacat
      361 tggattgttt tcccgccact gccaggactg acggaataac tccaccagca tgctgttatg
      421 cgttgcttcg gcaatagcga gatggaactg catgtcaccg ctttcgctgc tgcccggcgc
      481 actggaagcc agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc
      541 gcgggtagcc tgcaaagcgg caaactcggc gatgttgctc tccaataact gccgcgcctg
      601 taacagctca aaaggaccgg catcgttgca gacgttggca tccggactgt ctgtgttctg
      661 gctgcctgag ttatcaagaa catagatacc ggcaccccgg cgtacttcca ccagcccttt
      721 gatctccagc atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc
      781 aatttcacgc tccggcggca gccgttcgcc aggattgtac ggcgtcttta tgatcagatc
      841 gcggatcatc gccccgactt cctggtaagg tctttgcgca gaggtggcag atttcatggt
      901 gttgtgtccg ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc
      961 cggcctacaa cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg
     1021 aagttggcac gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat
     1081 tgaccagtaa gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac
     1141 cgcgttcgca cagctgctga tatgccgccg tcactgcgcc aacaaagtcg gcattctgcg
     1201 gcagatcatc ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct
     1261 gatactgcgc gttgaccttc tggaactccg ccagcatcgg gtcgaccacg tcaatggcat
     1321 taccctgctc atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt
     1381 gacgccagct gccgccgttt tgcaggtgca gacgcaccgg gtccagcaga cgctgcggta
     1441 acttctggct gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga
     1501 aacgctcgat cagcagcgtc gcataggcgt tcaggtccgt accttccggc atcgacagcg
     1561 ttggcgcttg ttcctgcatc atcaaggaga aggccgcttt gcgataatcc gggttagtca
     1621 tggtgtcggc aatggtttca tagccgccga ggtaaccgag gtatgccagg aaagagtggc
     1681 tgccgttcag catacgcagc ttcatcattt cgaacggcac aacgtctgca acgaactgtg
     1741 cgcccacttt atcccagtcc gggcgaccgt taacgaagtt atcttcaatc acccactgac
     1801 ggaacggttc gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct
     1861 gcaaggtttc tggcgtcgcc gccggaacga tgcggtcaac catggtgcac gggaaggtga
     1921 cgttttcttc aatccatgcc gccagctgcg ggtcacgcgc ctgagccagt cccagtaccg
     1981 cgaccttcgc cacatggccg ttttcacgca cgttatcgca ggacatcacc gtaaacgctt
     2041 tcaacccttt ttcacgacgc agccgcaggg cttcgacgat gtaaccaatc gcggacttcg
     2101 gcgcagtcgg gttttccaga tcgtgcttga tcagtgggtt attgagatcc agctgaccgc
     2161 ttgccgcatc agcgcagtag cctttttccg tgactgtcag agagacaatc gccgtctgcg
     2221 gacgcgccat cgcattgaga ataccttcgc agccgtcgat ttccggatgc agcgcttctt
     2281 tcatcgaacc gataattttc agctcggtgc tttccgcgcc tttttccgcc acggtgtaca
     2341 gcagttgctg ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa
     2401 cttcgcagat accccagtcg ctgtcggtgc tttccagcag gtgatgggta tacagcgcct
     2461 ggtgcgcgcg gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat
     2521 gctcccatga cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact
     2581 ccagaatccg tcttcagaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca
     2641 tgccggtggg tattaacggc taaagaaagc gcgctggatc gccagttcga caccgcgaac
     2701 ttcggccagg cctttcagac gaccaattgc ggagtaacct gggttggttt tcttcttcag
     2761 gtcgtccagc atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc
     2821 cgctttacga cggtgttctt cttcaacaat cgctttcacc acttcgtaca tatcaacgtc
     2881 accgttcagg tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg
     2941 cagatgggtg aagtaaatac gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc
     3001 agcacgcacg ccgtaggaac cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt
     3061 atcaaccatc cactgcatat cttcaatggt ggaaacaatg cgcggcaggc caaggatcgg
     3121 gcgcggcgga tcgtccgggt gaaccgccat acgcacgcca acttcttcag caactggaat
     3181 aatcgctttc aggaagacgg caaagttttc gcgcagcttc gctttatcga tatctttgta
     3241 cagctccagg tgcttacgga actggtcgag ggtataccct tcttccgcgc ccggcagacc
     3301 ggcaatgatg ttacgcgtca gacgcgcttt gtcttcgtcg ctcatagtgg cgaagcgaac
     3361 agcagcctga gcaatttctt cttcggtgta atccgcttcc gcgcctggac gtttcaggat
     3421 atgcatttcg aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg
     3481 cagcacgtat tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac
     3541 ggtgcgaatg ccgcactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg
     3601 ctcatagttg ccagtgtggg ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga
     3661 ccacaccagg cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca
     3721 tacttcgccg ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg
     3781 gacatcagct aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat
     3841 ttttcctctt tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta
     3901 ggtatccgga tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata
     3961 agttctacct tttggtaaga catgtcaacc cgaattctga aattggttaa ccacatcaca
     4021 agaatttcac tctcatggtt gttgctgcaa attgcagcaa agggtaatcg atggttattg
     4081 atcagaggat gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca
     4141 aaataacaac ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctctgg
     4201 gtggtattcg gcattggcct gatgctggta ctgacaaaga ccgcaggttc agacacattc
     4261 gctgcattag caatgcctct tagataattt tgacctgtca gtaaaagaca cgttgaaaac
     4321 ctggggtctg ctggagctgg tcaactccgt ggttgggctg attattagca aggtagcgta
     4381 aaagaacacc agagcctgcc aatggcaggc tctgactgat gacaaagtct aaaaatgtgc
     4441 ccggacagtc ccctcgcccc tccggggaga gggttagggt gaggggaaca gaccagcaaa
     4501 gtgcgctttg tttatgccag atgcgacgct gacgcgtctt atctggccta caaagggcta
     4561 acgtgcaggt ttttagcttc aggtaatatt gcgtaactgc attagcaatg ccctgtgatt
     4621 tctttattga taaacaaaag tcacgccaat aatcgattgc acattccctg cagtcacctg
     4681 ccctccggta cgtgcgtaat ttgccgttaa tcccagactt accgccgaag tccctactgc
     4741 tcctaacgat accgtgttat tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc
     4801 gacgccctgc gctggtgaaa acgacgcggt attggtgaaa atcgagttgc ccgcatctac
     4861 ggttgtgccg gagaggtaat accccagatt ttggcttttc gcacaataaa cggtaagagg
     4921 aattggcact gaaccagggt agtccggcag agtaacggtg acatcacgag cagaaacatc
     4981 gcagccgcca gtaggcacca ccacatcatt attggcgtaa atattccaca caaactgaaa
     5041 atcatcgctg ttatagttgt tggtctgtcg caaaataagc acggcaatta atgagccagc
     5101 tttaatcgcc accccgcccg cactgctcac aggcgtcaaa taaagcgcca ccggccacgg
     5161 cttatccgtt ctcgaattat aaacaacgcg cggcgtttcg ctggtggtag gaaatggatc
     5221 gctactgcca ctatatttta cggtcccgga aaaattagat aacacgctgc cataagccga
     5281 gcctcgttgc agtgtgacat agtctgtaat ggtttccgga taatcgttat ggcaaaagat
     5341 ttgcgtcgaa agatccacga ccaggttttg ccccacattc acggcgggcg caaggtttac
     5401 ataaacattg gcgctgccac cgccaatagg gatagcggta ccattggcgg ttttacaggc
     5461 gaatgaccag gcatttaccg accagcccat cagcagtaca gcaaacaggg taataactcg
     5521 tttcattaca atcatctctt cgggttcagc tgtaggtata ggtgatgcta atcactgcct
     5581 gaatggttcc ctgagtggct ccgccattta ccgtcaatgc tctgacctgt aacgggaagt
     5641 gcgctgattg tgaggaatca tccacctgaa ctgttttggt tgcgccagta ttcaatgtgt
     5701 tgccactgtc atcctgtagc tctaactgga tgttttgcgc ggtcccctgg tttttataat
     5761 atccggtact gtcggctgcc ccgctgaagc tggcggtgac cctcgacgtt cccaccggac
     5821 aattagtcaa ctcaagcgca acatcatgcc aggccgatgc cgccccggca gacatcagac
     5881 tgaaagaata aagatcgccg agatcaaccg tggcattggt ggtggaaacc gtacacggtt
     5941 tggcgacgac cttaccgttc accgtgatgg tgacatcggc tgcctgtatc gtcgcacttg
     6001 cgagcgccaa tattgccgcc aataaatacc cacgtttgcg ccatttcatg agcatctcca
     6061 gttactgata ttcaagagtg aaggtagccg tggcattgat atgccccgca gtgacaggca
     6121 cctgtgtcgc cattagccgg gcgtaaaaat tcaacgtatt tggtttaccc ggcgtcaggg
     6181 tcgtccacga aatcgcggac gatggagcat taaggggtat ttgattttgc tgctcattca
     6241 gaagctgtat tcccagtccc gaagccgctg acaccgtatt ttcaagtgca agcagattgg
     6301 cattgtggct atctgcaacg ccggtaaacc caacttttac ggcagaaacg gcattaccac
     6361 agggtgacag caaaatacga aatggaacaa caggagtcgt cgcgccaatg ttgttaaatt
     6421 gcttcgccgc gttttccatc agatcaacag taaaattggt tgattcagcg gccacactac
     6481 agccgttatc tctgacatag ccgcggatag taatcgtgct atccgcagcc aaagcgtgac
     6541 tcaccgccag ccacaaaaaa gcgcacagaa gataaaaagg tttgtttctc atcacgcccc
     6601 cttaacgaca ttcagctgat agctgggtta ataactgctg ctgactctct ggtggcagtt
     6661 gataattggc gacacagtga gcattttcct cttctcccca tttcacctga acttttcccg
     6721 ctaaaggcat tccgctgagg taaacctgac cattatccgc aacaatgccg ctactctggc
     6781 tactctctga tgtcaccatc gccccaaacg gcagcggctt attattgtgg gttagcgtca
     6841 tgagcagttt tatcccaacg cgcgctttaa actctgctcg cacgatcgcc ccacgagtgg
     6901 gaacaacgtt agcgaccgcg ttatctaaat cgacgttatc agccagggta ttggtatcca
     6961 gcgccactct gttttcccta tattcagtgg cataaggcag cacggcataa ccgcgccagt
     7021 cggtacgcac ccccgtctgg ttttcgactt ttgcatcttt tgcgccaggc gctttaacaa
     7081 gcaccaccgt atcgtttaac ggctgcccca gcgttacgcc attggcatga gccaataccc
     7141 caccgctgac tccgtaatag agctgcttaa tatcatcgct atggctgtaa ccgatattgg
     7201 cattgccgta accaccgcga taattcagcg tggcgtagcc tgtgcttccg ctattaccat
     7261 cgcctccccc ggcatagccg gtttgcacgc tatagctgag gttgttgtct tccagcaacg
     7321 taccgtatac accagccaga ttggtcatcc gaccgttgag atcgtgtgac atgctgtagc
     7381 tggcactggc atgtcgccac tgagatttac tgtcagaacg cagccagtgg ctgaaaggaa
     7441 tattgacgtt aagcgctaac atctgatcac gtcctttttg ccaggcgttt ttcgtcaggc
     7501 tatagctgag cgtccagttg atatcttcga acgcagtatt taatccagcc tggaattgct
     7561 catcgacatt actcgttccc caataagttt gatggctacc actcaaatac agtgttgatg
     7621 tgcgcccgag ttgctgagta acggtgagct gtaatttccc gcgtttgtta taagcgaggt
     7681 tgtaatagtc ggtgaatttc ggcttaacct gaataactcc atcctgtgtt tcgatgttgt
     7741 agccattcat tcgactgtat gttgtatcag cgaaattaaa atatccgctg gtcgaataac
     7801 ggtaacccac taactgaata ttcgtgcctg attcattgag cgatttgtta tagagaaaac
     7861 gcaccgattg tccgtcatgc tgactgtcat cgggaagtgt ggaattagcc tgcgtcatat
     7921 ccacagacag agcgcccagt gcccccatgt ttttcccgat accgaaatta aaagcacgat
     7981 aacgatccgc cagttgcgtt ccaccatata ttgtccagcc agccggaagg ccgtggagta
     8041 atgtactctg gaaaaagcgg ggtttttcct gctgcgcatt tccactacgg tattctcctg
     8101 ccgtaatgga ataacgagta tgcccttcac gttgcaaaag cgggactgac gaatagggta
     8161 cggtaaaaat ctgcgtgctg ccgtcagcct ctttgatcgt tacctgcaag tcaccactat
     8221 tacctgcggc atagatatcg ttgatggtaa aaggccccgg tggcaccgta ctattataaa
     8281 tgtcataccc attttgttta atagtgacct gtgcagtacc acgagcaata ccgtggatca
     8341 ccggggcaaa tcctctttgg ctatcgggta acatattgtc atctgaggcc aattgtgcgc
     8401 cgcgaaagtt aataccatcg aaaatatcgc cctgagtata accatcaccc agcgtcagcc
     8461 gggaacgtaa cggtattatg tctcgctcaa gccaggtatt gatatgctgc catttatttt
     8521 tgctacctga tgatctgtcg ctactgttat aactccaggt ggtattgtcg cgtaaacgcc
     8581 acgcaccaat atttaaccca ctctgtaggt ttaaatatgc ataatggctg ttacccccaa
     8641 tccgattctg tacactattt ccgctgaaat tataattgag caatccggca ttaataccgg
     8701 gatcccataa ctcaggagga atataaccac gcgcgcgatt actcataaat gcctgaggga
     8761 tcgtcaggtt cagtcgctgc tgaccaacat ccagatgcgc agtagcgtcc tggaccattg
     8821 tggttaatgg cacacaggca tcatccgcca gcagattcat accggcgaca gaagccgtat
     8881 tcagccccat actggcgagt tgcgcgcgtg tcaggcaggg aacaatccct tgttcactgt
     8941 cgcccgtatt aaatgtgaca tcacgcgttg ccatataacc attattcaaa tagatatcga
     9001 cgcgatacgt ccctggcggt aattcttgcc cattttcaaa acgcgataaa tcggccacag
     9061 cctggggatc atccgctaaa aaacgcgggt taaaatagag ttcggcagat gacaaaggtg
     9121 cctgtgcggc aaaagcacag gcaacaaaga gccggacaaa aaaaccagcc aaacgatgct
     9181 tacgaatatg caagcattgt gtgtttcgct ggtaaagtct taaattcaga tatgacatta
     9241 ttcctccggt ttaaagtcac ccggcagtca attcttttta ttcaggcgaa aaattccccc
     9301 tgcgttattc cattacgccc gtcattttgg gggtaagtgc accataatca tttattgttc
     9361 ggtaagtaat attgcttccc gcatcagaag gcaatttaac cgtgctttcg cccattggag
     9421 gcaccaaggc attttcaaga acccgggttc cggcattcaa ctctgttacc gtcaggtaat
     9481 agggtgtcgg gttaatcagc gtcagagaat tcgcgctacg acgaaatctt aatttttctg
     9541 cggcctgatc gggtggcaac gctaatttag ccgggcgata gtacagttta atgcggctga
     9601 taattgcgag ctgtagcgta ttctcagtca attttgattt atccattgac ggaatcgctt
     9661 taacattcat ccagaataaa ctttcccgat cctgtggcaa ttggttattt gttgcatcaa
     9721 gaatacgcaa ggtattctct ttttttccct tcatcgcaaa cagaggaggc gtcacgataa
     9781 aacgaccatc ctttacacca tcggcatttt ccacccatga ttgaattaaa taggtactat
     9841 tttcatcatt atttgtcacg gcaagttgca cttgtttttg ccctgccgga taaattacgc
     9901 gagtcgcacc taaggccact cccgcttcag cgcgtccggc aaccatcatt gccataaaca
     9961 tcaggatacc tgccagcaag cagaatgtta tttcctgcga tttccttaca ttgacgtttt
    10021 tattactcac tgtcctgttc ctgttatcac attatctgct gaacaattac tgataggtta
    10081 aagagaacca ggcctgggca ttggcgatgc cgccagtaac ccgacgcccg gtagcacgat
    10141 atttggcgat gaaatgtagc gaagtagagc ctgagtaaag ccgtttccag tttgctggag
    10201 gacgattaat cggtacgagg tttccttcat catcaaacaa cgctacgcca atattggtgg
    10261 ctatccctgg cccctctccc acggaaagca catccggatt tttaccatcc gcgacaccgt
    10321 gaaacgccac ccccacacgt tcactcacca ccgtgctaca ttcccgtaaa tgaataacaa
    10381 aaggcaccgg tgcgctatct tcccctgccg catgaaaccg gttactgctg atttgcccca
    10441 tattgaccgt catttgttta tcaccggctt caatccggca agtttccgca ataatgacgc
    10501 cctgaaattg catatttccg ccgggcagcg tggtattcca tttatttccg gccagagcaa
    10561 acattggcag caacgacgct aacagaaata atctcttcct tttcatcgtt atttttatcg
    10621 cacaagggtg ggcatccctg cccgtaatga cgtccttgaa cctgggtagg ttattgatac
    10681 tgaaccttga aggttgcatc cgcattagct gcacccgggg ttgcctcgcc gattgcataa
    10741 taacgcgcct ggaacggaat ggtgttggta ccgttattca gggttgtttg tgcactaaat
    10801 gtcgcaccgt ccagcgtcag agcattgcct gttctgtcca ggatctgcac gccaacgttt
    10861 gttgcactac ctgcagcaga actctgcaga gccagtacat cagtacgcgt agcatcaatt
    10921 gccgtaccta agaaggcaac agcggctttt gtggcaacag tggtatcgca atcattcaac
    10981 tgaatgttaa aaccaacggc agagctggtt gctccagcct gcttcaggct agcggtacga
    11041 acctgtccca actgaacggt ttgatcaaca gagcctgcat caactgcgca agcggcgtta
    11101 acaacttccc ctttaaagtg aacggtccca ccatttaccg tcgtagtatc ggccagagcc
    11161 gctgcggaac tgagggacag agccgacaga acaacgattg ccagagtttt aattttcatg
    11221 ctgctttcct ttaaaaaaac tatttctaaa tcgacatggg cagtcgttct gtacactttg
    11281 ttttgtcaac gagtttgact gccaacactg cacagttttc ccccaaaaga tgaaacattt
    11341 ggggccattt tgactcatag aggaaagcat cgcgcacaaa ctttttcagt tta
//
LOCUS       DAZFRJ010000142        11362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000142 DAZFRJ010000000
VERSION     DAZFRJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.143"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            243..1631
                     /gene="narU"
                     /locus_tag="ACHKUN_003466"
     CDS             243..1631
                     /gene="narU"
                     /locus_tag="ACHKUN_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO6661665.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1713..5453
                     /gene="narZ"
                     /locus_tag="ACHKUN_003467"
     CDS             1713..5453
                     /gene="narZ"
                     /locus_tag="ACHKUN_003467"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO6661666.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5450..6994
                     /gene="narH"
                     /locus_tag="ACHKUN_003468"
     CDS             5450..6994
                     /gene="narH"
                     /locus_tag="ACHKUN_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6661667.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6994..7689
                     /gene="narW"
                     /locus_tag="ACHKUN_003469"
     CDS             6994..7689
                     /gene="narW"
                     /locus_tag="ACHKUN_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6661668.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7686..8366
                     /gene="narI"
                     /locus_tag="ACHKUN_003470"
     CDS             7686..8366
                     /gene="narI"
                     /locus_tag="ACHKUN_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6661669.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8445..9338
                     /locus_tag="ACHKUN_003471"
     CDS             8445..9338
                     /locus_tag="ACHKUN_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO6661670.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9434..10279)
                     /gene="nhoA"
                     /locus_tag="ACHKUN_003472"
     CDS             complement(9434..10279)
                     /gene="nhoA"
                     /locus_tag="ACHKUN_003472"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO6661671.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10452..11021
                     /locus_tag="ACHKUN_003473"
     CDS             10452..11021
                     /locus_tag="ACHKUN_003473"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO6661672.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11025..11252)
                     /gene="pptA"
                     /locus_tag="ACHKUN_003474"
                     /pseudo
     CDS             complement(11025..11252)
                     /gene="pptA"
                     /locus_tag="ACHKUN_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2675 a         2956 c         3010 g         2721 t
ORIGIN      
        1 gaagggttga aacatgacat acgttcaaag attaaccccc gtttgaaggt tagcttgtta
       61 gttacattta gtaacacata tctggctact taatccccat tctgcccccg cggttacgct
      121 ttcttatcta ttctttcaat cagcacttca cccaattcat ccttaattct aataactggc
      181 tattgcgcct tacctcctgt tttgtctttt ttcattacca atgtgtgcat gtgaggaaca
      241 atatggcact gcaaaatgag aaaaatagtc gttatctttt acgcgactgg aaaccagaaa
      301 atccggcctt ctgggaaaat aaaggaaaac atattgctcg aagaaacctc tggatatcag
      361 tcagttgtct acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt accgttaatc
      421 tcaataaaat cggttttaat tttactaccg atcaactctt tttattaacc gcattaccct
      481 ccgtttctgg cgcattattg cgtgttccct actcctttat ggtgcctata ttcggtggac
      541 gccgatggac ggtttttagt actgcaatcc tgattattcc ttgcgtctgg ctcggaattg
      601 ccgtgcaaaa tccgaatact ccttttggga tatttatcgt tatcgctttg ctatgcggtt
      661 ttgcaggtgc aaactttgct tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca
      721 aacaagggag cgctcttggg attaatggcg gattaggaaa cttaggtgta agtgtaatgc
      781 agctggttgc accgctggtc atttttgtac ctgtatttgc ctttctcggc gtcaatggcg
      841 taccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc
      901 tactggcgat tgccacgatc gccgcatggt cagggatgaa tgatatcgcc agttcacgcg
      961 cctcaattgc cgaccagctc cctgtcttac aacgcctgca tctctggctg ctgagcctgc
     1021 tttaccttgc caccttcggt tcgtttatcg gtttttctgc gggttttgcc atgctggcaa
     1081 aaacccagtt cccggatgtg aatattctgc gcctggcgtt ctttggccca tttatcggtg
     1141 ccatcgcgcg gtcggttggt ggtgctattt ccgataagtt cggcggcgtg cgggtgacgt
     1201 tgatcaactt tatttttatg gcgattttca gtgccctgct gttccttacc ttaccgggca
     1261 caggctccgg taatttcatc gccttttacg ccgtatttat ggggctgttt ctgactgcgg
     1321 gtctgggaag tggttctact ttccagatga tcgccgtcat ctttcgccag ataaccattt
     1381 atcgggtaaa gatgaaaggc ggtagtgatg agcaagctca gaaagaagcc gtcaccgaaa
     1441 cggcggcggc tctgggcttt atctcagcca ttggcgcagt gggcggcttt tttattccgc
     1501 aggcgtttgg catgtcgctc aatatgaccg gctctccggt cggcgcgatg aaagtgtttt
     1561 taatcttcta catcgtttgt gtgctgctga cctggctggt ttatggtcgg cggaagttca
     1621 gccaaaaata agagatcgat gcctgatgta caaccgtgtc gcatccgaca aggcatcaga
     1681 tcccaattca ttcctggagc aggagtcatg tcatgagtaa acttttggat cgctttcgct
     1741 acttcaaaca aaagggcgaa acctttgccg atggtcacgg acaggtgatg catagcaacc
     1801 gcgactggga ggacagctat cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc
     1861 acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga
     1921 cctgggaaat ccaacagacc gactacccgc gcactcgccc tgacctgccc aatcatgaac
     1981 ctcgcggctg cccgcgtggc gcaagttact cctggtatct ttacagcgct aaccgcctga
     2041 aatacccgct cattcgtaaa cgactgattg aactgtggcg cgaagccctc aagcaacaca
     2101 gcgatccggt actggcgtgg gcatcgatta tgaacgatcc gcaaaagtgc ctgagctaca
     2161 aacaagtgcg tgggcgcggc gggtttatcc gctccaactg gcaggaacta aaccagctga
     2221 ttgccgccgc taacgtctgg accatcaaaa cctacggccc ggatcgcgtt gccggtttct
     2281 cgccgatccc ggcgatgtcg atggtttctt acgccgccgg aacgcgttat ctgtcgctgc
     2341 ttggcggcac ctgtttaagt ttctacgact ggtattgcga cctgccgccc gcctcgccga
     2401 tgacctgggg cgagcaaacc gacgtaccgg aatctgccga ctggtataac tccagctaca
     2461 tcatcgcctg ggggtctaac gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg
     2521 aagtacgcta caaaggcact aaaaccatcg ccattacccc tgactactct gaagtggcca
     2581 aattgtgcga ccagtggctg gcaccgaaac aaggcactga tagcgccctg gcgatggcaa
     2641 tgggccatgt gattttaaaa gagtttcatc tcgataatcc cagcgactac tttatcaact
     2701 actgccgccg ctacagcgac atgccgatgc tggtaatgct ggagcctcgc gacgatggta
     2761 gctacgttcc cgggcgcatg atccgcgcat ctgacctggt ggatggactg ggcgaaagca
     2821 acaatccgca gtggaaaacc gtagcagtta ataccgcagg tgaattggta gtgccgaacg
     2881 gttcgattgg tttccgctgg ggagaaaaag gcaaatggaa tctggaatcc attgccgccg
     2941 gtacggaaac cgaattgtcg ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg
     3001 ccttccccta ctttggcggc attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg
     3061 tgctggtgcg ccaattgccc gttaaaaacc tgacgttagt cgatggcaac acctgtccgg
     3121 tggtcagcgt ttatgatttg gtactggcga attacggcct cgatcgcggg ctggaagatg
     3181 aaaacagtgc gaaagattac gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa
     3241 ttaccggcgt gccgcgccag tatattgaaa ccatcgcccg tgaatttgcc gataccgccc
     3301 ataaaacgca tgggcgctcg atgattatcc tcggcgcagg tgttaaccac tggtatcaca
     3361 tggacatgaa ctaccgcggg atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa
     3421 gcggtggcgg ctgggcacac tatgtcggcc aggaaaaact gcgcccacaa accggctggt
     3481 tgccgctggc ctttgcgctc gactggaacc gaccaccacg ccagatgaac agtacctcgt
     3541 ttttctacaa tcattccagc caatggcgct atgaaaaagt ctctgctcag gagttacttt
     3601 caccgctcgc cgatgccagt aagtacagcg gtcatctgat tgatttcaac gttcgcgccg
     3661 aacgtatggg ctggctacct tctgcgccgc agctggggcg taacccgctc gggattaaag
     3721 ctgaagccga caaggccgga ttatccccca cagaatttac cgcccaggcg ctgaaatcgg
     3781 gcgatttacg tatggcctgc gaacaaccag atagcggcag caatcatccg cgtaatttgt
     3841 ttgtctggcg ctctaacctg cttggctcct ccggcaaagg ccacgagtat atgcagaagt
     3901 atctgctggg gaccgaaagc gggattcagg gcgaggaact cggtgccagc gacgggatca
     3961 aaccggaaga agtcgagtgg caaactgcgg cgattgaagg caagctcgac ctgctggtga
     4021 cgctcgactt ccgcatgtcc agtacctgcc tgttctccga tatcgttctg cccaccgcca
     4081 cctggtacga aaaagacgat atgaacacct cggatatgca tccgtttatt catccgcttt
     4141 ctgcggcggt cgatccggcg tgggaatcac gcagcgactg ggaaatctac aaaggtatcg
     4201 ccaaagcatt ttcgcaagtg tgcgtgggtc atcttggcaa agaaaccgac gtggtattac
     4261 aacccctact gcatgactct ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc
     4321 gcaaaggcga atgcgatctg attccaggca aaaccgcgcc gaatattgtg gcggtggagc
     4381 gcgactaccc tgctacgtat gaacgcttta cctcgctcgg gccattgatg gacaaacttg
     4441 gcaatggcgg taaagggatt tcgtggaata cgcaggatga aatcgatttc ctcggcaaac
     4501 tcaattacac caagcgtgat ggcccggcac aggggcgtcc gctgattgac accgccattg
     4561 acgcttcaga agtgattctg gcactggcac cggaaaccaa cggtcatgtt gcagtcaaag
     4621 cgtggcaggc gctgggcgag atcaccgggc gcgaacatac ccatctggcg ctgcacaaag
     4681 aggacgagaa gattcgcttt cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc
     4741 ccacctggtc cggtctggaa agcgatcacg tatcctataa cgcgggatac accaacgttc
     4801 atgagttaat tccgtggcgc acgctgtcgg gacgccagca gctctatcag gatcatccgt
     4861 ggatgcgtgc ttttggtgaa agcctggtgg cttatcgccc gcctatcgac acccgtagcg
     4921 tcagtgagat gcgccagata ccgccaaacg gcttcccgga aaaagcactt aacttcctga
     4981 cgccgcacca gaaatggggc attcactcaa cctacagtga aaacctgcta atgctgacgc
     5041 tctctcgcgg tggaccgatt gtctggatca gcgaaacaga tgcccgagaa ctgaccattg
     5101 tcgataacga ctgggtggaa gtattcaacg ccaacggcgc gctgactgcc cgcgcggtgg
     5161 tcagccaacg tgtaccgccg ggcatgacca tgatgtatca cgcccaggaa cgcattatga
     5221 atattcctgg ttcggaagta actggcatgc gcggcggcat tcataactcg gtcacccgcg
     5281 tttgcccgaa accaacgcat atgattggcg gttacgcgca gctggcctgg ggctttaact
     5341 actacggcac cgtcggatcg aaccgcgatg agttcatcat gatccgcaag atgaagaacg
     5401 ttaactggct ggatgatgaa ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatccg
     5461 ttcacaagtc ggcatggtgc ttaacctcga caaatgtatc ggctgccata cctgttcggt
     5521 gacctgtaaa aacgtctgga ccgggcgcga aggcatggag tacgcatggt ttaacaacgt
     5581 cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag agtggcaagg
     5641 cggctgggtc cgcgatgtga atggcaagat ccgtccgcgt ctgggcagca agatgggcgt
     5701 aataaccaaa atcttcgcca acccggtggt gccgcagatt gatgattact acgaaccttt
     5761 caccttcgac tacgaacatt tgcatagcgc accggaaggc aaacatatcc ctactgctcg
     5821 cccgcgttca ctgattgacg gcaaacggat ggacaaagtg atctggggcc caaactggga
     5881 agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg aggccatgca
     5941 aaaggagatg tacgggcagt ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga
     6001 acactgcctc aatcccagtt gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga
     6061 agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt tgtgcattag
     6121 cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag aaaaatgtat
     6181 cttctgttat ccgcgaattg agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg
     6241 tcgcatccgg tatctgggcg tgctgcttta cgacgccgac cgcattgagg aagcggcgag
     6301 caccgagcgc gaagttgacc tctatgaacg tcagtgcgaa gtattcctcg atccacacga
     6361 tccctcagtg atcgaggaag ccctgaaaca aggtattcca caaaacgtga ttgaagctgc
     6421 ccagcgttcg cctgtctaca aaatggcgat ggactggaaa ctggcgctac cgctgcaccc
     6481 tgaatatcgc accctgccaa tggtctggta cgttcctccg ctgtcgccga ttcagtccta
     6541 cgcagatgcg ggcggtttgc cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg
     6601 tattccggtg caatatctcg ccaatatgtt gagtgccggc gataccggtc cggtactgcg
     6661 ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt
     6721 tactgatact cgtgccatcg acgaagtagg cctgagcgtc gcccaggtcg aagagatgta
     6781 tcgttacctc gccattgcca actatgaaga tcgttttgtc atcccgacga gccatcggga
     6841 aatggcgggc gatgccttcg cagaacgcaa cggctgcggt tttacctttg gcgacggttg
     6901 ccacggttcg gacagtaaat tcaacctgtt caacagtagc cgtatcgatg ccatcaacat
     6961 caccgaagtg cgcgacaaag cggagggcga ataatgcaga tcctcaaagt gatcggcctg
     7021 ttgatggagt atccggacga gctgttgtgg gaatgcaagg aggacgcgct ggcgttgatc
     7081 cgcagcgacg cgccgatgct tacggatttc acccacaacc tgcttaacgc gccgctgctg
     7141 gataaacagg ccgaatggtg cgaagtgttt gaccgtggac gtaccacatc gctgctgctg
     7201 ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg caatggtgga cctgctggcg
     7261 gagtatgaaa aggtcggcct gcagctggat tgtcgggaac tgcccgatta tctaccgctg
     7321 tatctggagt atttaagcgt actgcctgac gatcaggcga aagaaggatt gctcaatgtt
     7381 gcgccgatcc tcgccctgct tggcggtcgc ttaaaacaac gcgaggcacc gtggtacgcg
     7441 ttgtttgatg ctctgctgca actggcggga agctcccttt caagtgacag tgtcacaaaa
     7501 caagtaaaca gcgaagagcg cgatgacacc cgccaggcgc ttgatgcagt gtgggaagag
     7561 gaacaggtga agtttattga agataacgcc acggcgtgtg acagctcgcc gcttaatcaa
     7621 tatcagcgac gctttagcca ggatgtcgcg ccgcaatatg tcgacatcag tgcgggaggt
     7681 gggaaatgat tcagtatctg aacgtctttt tttacgatat ctacccgtac atttgtgcga
     7741 cggtgttttt cctcggcagc tggctgcgct acgactacgg gcagtacacc tggcgcgcct
     7801 cctcaagtca aatgctcgat aaacgcgaga tggtgatatg gtcgaatctg ttccatatcg
     7861 gcattttggg gattttcttc gggcacttgt tcggcatgtt aacgccgcac tggatgtacg
     7921 cgtggttttt gccagtggca gcgaaacaac taatggcgat ggtgctcggc ggtatttgcg
     7981 gtgtgttgac attgattggc ggcgcaggac tgctgtggcg caggctgaca aatcagcgcg
     8041 tgcgtgcaac ttccactacg ccggatatta tcatcatgag cattttgctg atccagtgtc
     8101 tgctggggct aagtaccata ccgttttcag cacagtatcc tgatggtagc gaaatgatga
     8161 agctggtggg ttgggcgcaa agcattgtca ctttccgtgg tggatcgtca gaaatgctta
     8221 acggcgtagc gttcgttttc cgcctgcatc tggtattggg aatgaccatc ttcctgctct
     8281 tcccgttcac cagattggtg catgtgtgga gcgcgccgtt tgagtacttt actcgtcggt
     8341 atcagattgt gcgttcgcgg cgataatttc accctggccg gatagccctt cgctctccgg
     8401 ccacttgcag gctatgatag ttctccagtc acaggagaac gttaatgaaa ccgcaggtgt
     8461 accacgtcga tgcttttacc tctcaaccat ttcgcggcaa ttctgccggt gttgttttcc
     8521 ccgccgataa tcttagcgaa gcgcaaatgc agcttatcgc ccgcgagtta ggccattcgg
     8581 aaactgcttt tctgctgcac agcgacgaca gcgatgtgcg catccgctac tttacgccaa
     8641 cggttgaagt gccgatttgc ggtcacgcga cagtagctgc gcactatgta cgtgcgaagg
     8701 tgttaggttt aggaaattgc acgatctggc aaacatcgct ggcaggaaaa catcgcgtga
     8761 ctatcgaaaa gcacaacgat gattatcgta tttcgctgga acaaggtacg ccgggctttg
     8821 agccaccgct ggaaggtgaa acacgtgagg cgattatcaa cgcgcttcat ctcactgaag
     8881 acgatatttt gccgggcttg ccaattcagg tcgcaacaac aggacactca aaagtgatga
     8941 tcccgttgaa accggaagtg gatatcgacg ccctttcgcc tgatcttgct gcgctgagcg
     9001 ctatcagcaa acagattggc tgcaatggtt tcttcccgtt ccagatccgc ccaggcaaaa
     9061 acgaaaccga tggtcgtatg ttctcgcctg cgattggtat tgtggaagat ccggtgactg
     9121 gcaatgccaa tggcccgatg ggtgcatggt tggtacatca caacgtattg ccccacgatg
     9181 gcaacgtgtt atgtgttaaa ggccatcagg ggcgcgcatt ggggcgtgac ggcatgattg
     9241 aagtgacggt gacaattcgt gataaccaac cggaaaaagt caccatttca ggcacagcgg
     9301 tgattttgtt tcatgccgaa tgggcaattg aactctgaaa ccgttgcctg atgcgcttcg
     9361 cctgtcaggt ctacaaccga gctaatgtat gccggataag gcgtttacgc cgcatccggc
     9421 aacctgacat aaattatttt cccgcctccg ggtgcgtatc aaacgccgcc atcaccagcg
     9481 ctaactcatc cacggtaaag ccatgtttcg catcatccac gcccagacca aactgttctt
     9541 gcatcacagc atataatgac gccacatccg gtagatttcg ctgctccacc gcgtgcccat
     9601 tttcataatg ggtaaaatga aaattggtca gcgttagctt gccaccgtcc ggcaaatggc
     9661 ggcacatcag caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac cagaaattgc
     9721 ccatcacata atcgctttgt tgctgctcgc agagatcaaa acggtacatc gactgccagt
     9781 gctgatgatg attaaactgc aacacccaat catcaccctc ctgcaacaac cgataatctc
     9841 cgtgtggcgt ggtctgcacg agatcggaaa ctaaacgaat cggcgcggtt agcgtctgcc
     9901 caccgaaacc gacatcagca atccattttt cctcttccag ttccaccaac agcaaacgat
     9961 gggtgcgcgg cggtaatgct ggcggatttg ataacactac gcgccctaac aagctgcgaa
    10021 cgttaaactc cagctcgcgt aacacccgct caaacacgcc attctgctca aaacagtaac
    10081 cgccacgacg ggctatcacc agtttctctt ccagcgattg atcatcaagc tgtatttccc
    10141 tcggcagcaa aacgtcgagg ttttcaaacg gaatagtgca attgtgtttc aggtgcaatg
    10201 cacgaagcgt atcaatattg accgcagcag ctcccgacca gttaatacgg gcaaaatagt
    10261 gattcagaat gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt ataacctgga
    10321 atagacgccg caccggctat tttgtgcgat cgcggacatg catcacgttt gcaaatcgcc
    10381 aatcccgtta atttcgttac ttattgcgtt acggactacg cttaaaaaaa ccttcttcgc
    10441 gaggcgatac catgagccga ttcatcccca tagaattaca ccatgccagc cgtctgttaa
    10501 atcatggccc aactgtcatg atcaccagtt tcgatgagca atcccagcgg cgtaacatta
    10561 tggcagcagc ctggtcaatg ccggtggagt ttgaaccgcc acgcgtggcg attgtggtag
    10621 ataaatcgac atggaccagg gagttgattg aacgtaacgg taaatttggc atcgttatcc
    10681 cgggcgttgc agcaactaac tggacgtggg cagtgggaag cgtgtcgggt cgtgaagaag
    10741 ataaatttaa ttgctatggc attccggtgg tgaaaggtcc ggtgcttggt ttgccacttg
    10801 ttgaagagaa atgcctggcg tggatggagt gccgcctgct acctgcgact tctgcgcaac
    10861 aaaaatacga cacgctgttt ggtgaggtgg tatcagcagc ggcagacgca cgggtatttg
    10921 tcgaaggccg ctggcagttt gatgatgata agctcaatac gttgcatcat ttaggtgctg
    10981 gcacgtttgt taccagcggc aagcgagtta cggcgggtta agcattacat gctgtaaccg
    11041 ggtttcttta tcaaagcctc catttggggc gcgatttcgg catcccagat agcttgccaa
    11101 gattctggtt gaatctgctg tagagcaatg cttatcgagc tatctttact gttcagatgg
    11161 cgaataataa cgtcggtaat atctgcagca agtgctgctt tttattgttc gtccagttca
    11221 cgcggaaaac atttaatgtc gatgtgcggc atgattgtgc tccttttgaa agatgtcagt
    11281 aaaattaaca cacattgtga atgtcgtgtg tccggtaatg cgtttttacg acaataatgc
    11341 cagggcaaga ttacgaaagc ct
//
LOCUS       DAZFRJ010000143        11295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000143 DAZFRJ010000000
VERSION     DAZFRJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.144"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..1900
                     /locus_tag="ACHKUN_003475"
     CDS             122..1900
                     /locus_tag="ACHKUN_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO6661673.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1940..2419)
                     /gene="xdhC"
                     /locus_tag="ACHKUN_003476"
     CDS             complement(1940..2419)
                     /gene="xdhC"
                     /locus_tag="ACHKUN_003476"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO6661674.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2416..3294)
                     /gene="xdhB"
                     /locus_tag="ACHKUN_003477"
     CDS             complement(2416..3294)
                     /gene="xdhB"
                     /locus_tag="ACHKUN_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO6661675.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3305..5602)
                     /gene="xdhA"
                     /locus_tag="ACHKUN_003478"
     CDS             complement(3305..5602)
                     /gene="xdhA"
                     /locus_tag="ACHKUN_003478"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO6661676.1"
                     /translation="MEAREATATGESCMRVNAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6017..6772
                     /gene="actS"
                     /locus_tag="ACHKUN_003479"
     CDS             6017..6772
                     /gene="actS"
                     /locus_tag="ACHKUN_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO6661677.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6851..6924
                     /locus_tag="ACHKUN_003480"
     tRNA            6851..6924
                     /locus_tag="ACHKUN_003480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6883..6885,aa:Gly,seq:ccc)
     gene            7059..8666
                     /locus_tag="ACHKUN_003481"
     CDS             7059..8666
                     /locus_tag="ACHKUN_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6661678.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            8659..9318
                     /locus_tag="ACHKUN_003482"
     CDS             8659..9318
                     /locus_tag="ACHKUN_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO6661679.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            10227..10955
                     /locus_tag="ACHKUN_003483"
     CDS             10227..10955
                     /locus_tag="ACHKUN_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6661680.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            10957..11157
                     /locus_tag="ACHKUN_003484"
     CDS             10957..11157
                     /locus_tag="ACHKUN_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661681.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
BASE COUNT         2865 a         2765 c         2864 g         2801 t
ORIGIN      
        1 aaaatgataa aaatcttatg tttttatcaa agccaatttc gcctgagtta atatcatcat
       61 gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg aaggtaaagc
      121 tatggagctt gctactacgc agtcagtatt gatgcaaatt caaccgacaa ttcagcgttt
      181 tgccagaatg cttgccagcg ttttgcagct tgaggttgag atcgttgatg aaaacttgtg
      241 tcgcgtcgcc ggaacgggcg cgtatgggaa gtttcttggt cgccagttga gcggcaactc
      301 acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac agtctcgctt
      361 cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc cgtgaaaaag catttctggg
      421 tacgcctgtc attttacagg atcgttgtgt tggggtgata agtttgattg ccgttaccca
      481 cgagcaacaa gagcatatca gtgataattt acgcgaattt tctgattatg ttcgccatat
      541 atccaccatt tttgtttcga aacttctgga ggatcagggg ccaggagata acatcagtaa
      601 aatattcgcg accatgatcg ataatatgga tcagggcgta ttagttgttg atgatgaaag
      661 tcgggttcag tttgttaatc agactgcctt aaaaacactt ggtgttgtac aaaataatat
      721 tattgggaaa cctatccgtt tcagaccatt aacatttgag agtaatttta ctcatggaca
      781 tatgcagcat attgtttcgt gggacgataa aagtgaatta atcattggtc aattgcataa
      841 cattcagggc cgacaattat ttttaatggc atttcaccaa tcgcatacca gtttttctgt
      901 agcaaatgca cctgatgaac cacatattga acaattggtt ggcgagtgcc gtgttatgcg
      961 gcaattaaaa cgactcatta gccgtattgc acccagccca tccagcgtta tggtggttgg
     1021 tgaaagcggc acgggtaaag aagtcgtcgc ccgagcaatc cataagttga gcggaagacg
     1081 gaataaaccc tttattgcta tcaactgtgc cgcgattccg gagcagcttc tggaaagcga
     1141 actgttcggt tatgttaaag gcgcatttac tggcgcttct gccaacggta aaacagggtt
     1201 gattcaggcg gcgaatacgg gcacgctgtt tctcgatgaa ataggtgata tgccattaat
     1261 gttgcaggct aaattactgc gcgctattga ggcgcgtgaa attctaccga ttggtgccag
     1321 tagcccaata caagtcgaca ttcgcatcat ttctgcaact aatcagaatt tggcccagtt
     1381 cattgccgaa ggtaaattcc gcgaagatct cttctaccga cttaatgtta tcccgataac
     1441 tctgccaccg ctgcgtgaac gtcaggaaga tattgaacta ttggtgcatt actttttaca
     1501 tctgcatacc cgtcgtctgg gatcggttta tcctggcatt gctcccgatg tcgtcgaaat
     1561 attgcgtaag catcgttggc ccggaaacct gcgcgagtta agcaatttga tggaatatct
     1621 ggttaacgta gttccttcag gtgaagttat cgacagcacg ctattgccgc caaatctgct
     1681 gaataatggc acaacggagc aaagtgatgt aacagaggtc agtgaggcgc acctgtcact
     1741 cgatgatgcg ggcggcacgg cgctggagga gatggaaaag caaatgatcc gcgaggcgct
     1801 ttcacgtcat aacagcaaga agcaagttgc tgatgaactg ggcatcggca ttgctacgct
     1861 ctatcgcaag attaagaaat atgagttgtt aaacacataa cctcagtgaa tccggcctga
     1921 attcaggccg gatatccttt tacttcgttt tctcgcaatc cagaactgta tttacaatca
     1981 tctgataccc cgtgcagcga caaagatttc ccgccagtcc gcgacgaatt tccgtaatgg
     2041 ttaatggctt ctcgcgtggt ttcgccagca ttgccgtggt agccataatc aggccaggcg
     2101 tacaaaaccc gcactgcact gcgccggatt tcgcataagc ctgctgaaca tgagaaagtt
     2161 ttccgccttt cgcttcacct tccagcgtgc ggatctcttt tccttcagcc caggcggcaa
     2221 ggtataagca actgtctatt gctgtgccgt cgaccaacac cgtacaggca ccacattcac
     2281 ccacgcagca cccttgtttg acacttagca gtccttgttc gcggagtaat tccgagagcg
     2341 gcgtgcctgg tgcggcgtga agctgaaaag gcatcccgtt aatggtgcat tcgatggtaa
     2401 ttgtttcgct gtgattcatt gcaattttcc ccccgccgcg gcgacggctt cgctaatcac
     2461 ttttttggtc atcgtctgga tgagatgcag acgaaactct ttactggccc gccatgaaga
     2521 acgcggggcg acatcttgca ggacagattc gctgatagct tccagcgttt gcaggtttaa
     2581 tggcgcattt tgtgcagtct gttcggcatg ttggcagcga atcggcgttg gcgcggcaac
     2641 accaaatgcc aggcgtaatt cgctgaaatt gccgttatcc agtcggcaat gtgcggcgca
     2701 gccaatcgtt gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg cgctgcccgc
     2761 gtgttctttc ggctgtggcg gaaaatgaaa ggcgacgagg atttcgtcat gctcaagaga
     2821 cactttgccc ggcccggtgt gaaagccatt aatcgggacg aaacgaacac cgcgcgggga
     2881 gtggatctcc agtttcgcgt cataaattag cgttggcgtg gcagaatctg cgctggtggc
     2941 accgttgcaa atatttccac cgtaggtagc gacgttacgg atctgcggtc cagcaatgga
     3001 cgtggccgca gcacataacg ccgggagatg acgttgagtt ataggatctt ctattagctg
     3061 ggtaaatgtc gttgcagagc cgatacgtag cgagccatct tccgccagcg taattccccg
     3121 cagctccgcc agattatgaa tatcaacaat atggcgataa cggtcattgt gatggtggag
     3181 ctgaatcagt acgtcagtgc caccggcgag cagtttggcc tgcgggttgt cagccagcag
     3241 gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa acatgatgtt
     3301 atcctcaaat caatcctgcc agatggaact cttcatataa ccgttttggc gtcagcggca
     3361 gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt acgaatagca gcggcaacag
     3421 gaattattgg tggctcaccc agtgacttat gtccgtatgc ggattgcggc tcattgattt
     3481 cgacgaacgc gctttccagt tgtggcagat ccggcatggt cggcattttg taatccagca
     3541 gattggggtt acggaccacg ccgcttttag catcgatgat catctcttca aatagcgccc
     3601 agccaatgcc cattcccatt ccgccgtgta cctgaccttc tgccagcagt ggattaagaa
     3661 tatgccctga atcatgaacg ttgaggatgc ggttgatggt gactttgcac agcgcaatat
     3721 cgaccgtcag atcaacaaag gtacagccaa acgccggtgg gttagtggtg gttttgatgg
     3781 agctttcagc agagagctgc ccgccgcgtt cagggtggta gaaagcgtcc atcgccaaat
     3841 cttttaacga cattaacggc tcttccggtc gttcaaccag cacgatatgg ccttttatca
     3901 gggtcagatt catcgctgac tgatgtagca tgactgcggc gtgagcgatg attttctctt
     3961 ttaataatag tgccgcactg cgcagcgcag gcgcggcaac atagctctgg cgtgaggcaa
     4021 atgcgccggg atcgaacggc gtaacgtcgg tatcttgagt tgaaataacg cgaacgtcgc
     4081 tgaccggaac ccccacggtt tctgccacca tttgcgagaa gacggtgtcg gcaccctgac
     4141 cgatttccgt cgcgccgctt tgcacgttga tggttccatc ctgattcatc agaaggcgcg
     4201 cgcctgctat ttctacgccg acaggccagg tgttagaggt gtagctaaaa caggcgacgc
     4261 caacgccgcg gcgcaaattg ccttgctggt tctggcattc tgcacggcgt ttttcccatt
     4321 caaagatttt ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa atacgtttgc
     4381 ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg taaacgaatt tcaacaggat
     4441 caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc aacggcaaat acgacttgtg
     4501 gcgcgccata accacgcatc gcaccagccg aggggaggtt ggtatagcag gtctttgaac
     4561 tgtaagcgta ggcacaacga ggataaaggt aagcgacttt attccccccc gcagaagcga
     4621 tggagtgccc gtgagatgca taagcgccgg tgttagacag aacatccaga ctataacctt
     4681 tcaatgttcc gtcgcggttc acgcccattt gcccgtcaat ggtaaaagcg tggcgggtac
     4741 gggttgcgag gaaacactct tcacggctaa gggaaacttt caccggaatg ccgccaagct
     4801 tgctggtcag gaatgccgcc attggctctt ccagtacatc ctgtttatta ccaaaaccgc
     4861 caccgacaaa cggtttgatg actcgtacgc atgaccaggg aatatccagc gcctgaccaa
     4921 ccacgcggcg aacaatgtgc gggatctggg tgctggaaac gatggtaatt cgcgagtcat
     4981 cctccatcca cgccagcgat gttacgcttt ccatgtgaca atgttgaata acgggggtct
     5041 gatagtgccc ctgtacctgg tagtcggcgg catcgattgt ttgttggaca ttacccgtcg
     5101 acatcgtgct ttgtttcagt aaattgccac cgttatggat tggtgcagcg tcttctgcca
     5161 gcgccgcttc tggcgtggtg ataacgggta attcttgcca ctcaatgctg accaattgcg
     5221 ccgctttttc tgccgtgagt tcatcgcggg ccacgacgat ggcaacggcg tcaccatgat
     5281 gacgaacatg gcgagttagc agtgcgcgat cggcggtatc gcgcttgttt tcgtcaagtg
     5341 tccaggcatg cccagcagta gcaaatggaa tatcaggcac atcttcccag gtaaaaatcg
     5401 ccagtacgcc cggcaaactt ctggcttgtt catcattaat acttacggca taaccatgtg
     5461 cgatagggct acgtacatat ttcgcataac acatgcccgc cataacataa tcgtcagtat
     5521 atcgtgcccg cccggtgacc ttagcaatgg cattgacgcg catgcatgat tcacccgtag
     5581 cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg aacatttggt tcttatggca
     5641 aagatttagt ctaagcaggc aagatttacg ccagaaacga cgataagcgt taggggaggt
     5701 aggaagtgtg atgacgttaa aaaaacacag atatgatacg aaacaggact ctgaataggg
     5761 gcagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaattg ggattattac
     5821 gcatcaggat gggaaatagg gtaaaaagtc aggaagttcg atacctctac agcgaatatt
     5881 ttgctgatga tacttccctt tgccaaatga aattaacctc tttctctatt accacgtttt
     5941 tccagaagca agagattggg tatcaagagg ctggctgcta tgataaggcg tcttgttttt
     6001 taagcgagga aagattttga gtgcgggacg cctgaataaa aaatctctgg gtatcgtgat
     6061 gttgttatcg gttggactgc ttttggcggg ctgttcgggt agcaaatcat ccgatacagg
     6121 aacgtattcc ggctccgttt acaccgtgaa acggggggat acgctatatc gtatttcgcg
     6181 caccacggga accagcgtaa aagaactggc gcgactgaac ggcatttccc ccccttacac
     6241 cattgaagtt ggtcagaaac taaaactggg tggggcgaaa agtagcagta gtacacgtaa
     6301 atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag cggtaccgaa
     6361 atcttcctgg ccgccagtag ggcaacgttg ttggttatgg ccaacgacag ggaaagttat
     6421 catgccgtat tcgacagcag atggcggcaa taaagggatt gatatctcag ctccacgggg
     6481 tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc agctgcgtgg
     6541 ctacggtaat ctcatcatga ttaaacacag tgaagattac attacggctt acgcccataa
     6601 tgacacgatg ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa tcgccactat
     6661 ggggagcacg gatgcggcat ctgttcgcct gcatttccag attcgttacc gtgcaacggc
     6721 aattgatccg ctacgttact tgccgccgca gggcagcaag ccaaaatgct gatggcgaat
     6781 taatcagcag tcagcagcgc ggcacttgct aaggagagcg taaggtttat aatgccttac
     6841 gcatctcgaa gcgggcgtag ttcaatggta gaacaagagc ttcccaagct ctatacgagg
     6901 gttcgattcc cttcgcccgc tccatgacca ttcttaaagt ctactaactt actgaatcct
     6961 aagggatacg gcttaaagcc ttccgatagt tttactcact gtaaccaaca ctgtgactaa
     7021 ctttgttgaa gtagtgagca gacctaaggt ctcagtacat gctcaattca agaacctacc
     7081 tttatcagcg taacggtgtc ttttacatcc gtctcaggat gaagaccact ggtcgcctga
     7141 ccgcttcgct tccttctcac aaccggtata aactagcgtc agtatcacta cggactaagg
     7201 acagacgtac cgctatggct cactcacggc acatcaagtc agcacttaaa gcaatacacg
     7261 cagacaaccc tagcgcatca tatgaggaac tacgggagca tctgaagacc attgttgagt
     7321 gggaacttgg tgtcagccgt gatgacctga acgatccgga gtcctaccag ctttacgttg
     7381 accagtacga cgacatcaag tccaaccttc gggaagctgt agcgactgag cgtctgactg
     7441 tagaccagca tcgctatatc aatgacgtta tcggtgtact taaagcctgt caggatagac
     7501 tcaagggcga taactctggc ctgttgtctt accttgagcc tgagactggc agtctaagac
     7561 cgtccgtctc cctatctgta tcatcggagc ctgaagtccc tgaacctaaa gcactgaccc
     7621 tcgcgtctct cattgagcag tacgagcagg agaacgccca gaactggaag ccagcgaccc
     7681 taagcgaaaa ccgagcgtca cactctacgc taatcgagat attcgactat ctggatattc
     7741 aggatgtcgg caaggcgacc cgtgcagata tgcttagggt ccgtgaagtc ctccaacagc
     7801 taccgaagaa ccgcaagcaa cgctttaagt ctatgccgct gtctgacctg ctgaaccggg
     7861 agtctaagac tgactgtctt gatgtcgtta ccatcaacaa taaatatctt atcaagatgg
     7921 ctgctgtctt taagtgggcg gtacgtaacg acctgatagc taagaacctg accgaaggtc
     7981 ttgagctgaa agtcccgcag cgtaaagcct ccgacgctcg tgatgctttc agtcctgagc
     8041 aggtagggca actactggtc gccgctaaag cgtactctca gaagaccgca ggtaagccat
     8101 accattatta cgtcaccgct ctggctgcga ttacaggtgc gagacttaac gaggtggccc
     8161 agcttcaggt caaagacgtc agggctactg aggccgggac cgtatatatc catatcaacg
     8221 aagacgatag cagcctaccg ggtaaaagta ttaagaacgc tcatagcgac cgctgtgtcc
     8281 cgctggtcga tggcgcttat ggttttgtat tggctgactt tatgtcgctt gtggaagacc
     8341 gtagaaagac tgcgggtgat aatgctatgg tattcgatgg cctcaagctg atgaagaatg
     8401 gctacggtga gcaggtgagc aaatggttta acagaacgtt gctgcctaaa gtccttgctg
     8461 accgtagcgg cttagccttt cactcattca ggcacactgt tgcgacccag ttaaaacaac
     8521 atggggtcga gttagcgtat gctcaggcga ttatgggtca tagctcaggg tctataactt
     8581 acgaccgtta cgctaaagag gtcgaggtgg atagattggt taatgtgatg gcaggtgttt
     8641 ataaggagac tggattaaat ggctgagtat tatccggcgg tgtttgaagc agaggctttt
     8701 aactgtcctc actgtggtgt atatgcccgt cagttctggc gtaggatgta tggaagtgcg
     8761 cgtgagttgg cgcaaaggac aaccgagttc cgaatgtcaa catgctctca ttgcggtgat
     8821 gatgcttatt ggtatgacgg caatatgatt attccggcgg ctggcaatgt tgaactacca
     8881 aatccagata tgccagataa ctgcaagtcc gactatatgg aagcccgaag catcattaat
     8941 ctgtctccta aaggggccgc tgcactcctt cgtctttgcc ttcagaagct aatggtccac
     9001 ttaggcgagc cgggagaaaa cattaataaa gacatccggt cactcgtcca gaaaggacta
     9061 cctgtacgca tccagcaggc ggcagatatc tgtcgtattg tcggcaatca ggcggtccat
     9121 cccggagaga ttagtcttga tgatgacccg cagttggcac acggtctctt taagctgctg
     9181 aacattattg tcgatgaccg aatcacgaga ccgaaagaaa ttgaggctat gttccagagt
     9241 atgcctgaag gtccacgtca aggtattgag aatcaggaca gacaggcgag ggagcagcag
     9301 caggccgcta acgaatgaaa cccacactat aggggaaggg gggggactaa actatcacta
     9361 taggggagag accctaagtc cccccgggct taatacagcc accgacccac acattcatta
     9421 agtctcaggc ttccggtagc ggaccgattg gaaaccatcc gcaataagcc tagtgttaaa
     9481 tcaccttggc ctgagaccat tccctttaac gtagcaatct ctctcctttg acaggcggag
     9541 aacatcagac cgcagcggaa gtatccgtct atgcgaccat cggtgtgcga gattgtgaag
     9601 aaccaagaca tcattgctga ggtctgccat ccatttgtgg acaggcggaa actcaaaaag
     9661 gttagaccag tggtgtctga ggttggagct ttgagcctga ccagcaggct tgaggagacc
     9721 atgagttcat cttgttatgg attgtgttat cacaattcca ataagaagaa ctttaagtcc
     9781 agcctccagt cctgtcagcc cgaagtatac tacggtcgct atgatgtggt tctccatact
     9841 tcgggtctaa ctggaagtac tgaacacttt cgcgtcaggt tctccgaagt tcattcctgt
     9901 ttgactaaac tatcactata gggaccatat ctcgcctctg acctcctgag agacggcaag
     9961 cgagaaaccg tggtccacgc tagcttgcta gccaccagcc tctggtcagc ctttgtgttc
    10021 tttggtctcg tgacttaagt ctatcaccac acacgctcgg acatgctgtc gaaccttcat
    10081 ctctatggtc atcgcagacc actaacccca attacctcaa gtcattctgt ttgcgtcctg
    10141 ttgcctgctg gtctcccttt acgtgggggc ttgagtaggt cagtgggggt gcgacttcag
    10201 ggtgcaaaca aaaggagtac acgtaaatgt ctcaacaaga aatcagcgtc attaacctcg
    10261 accagcttgt ctccatgacc tcagttgaaa ttgctgagct gacaggcaag gagcaccgaa
    10321 atgtcctacg tgatattcgc aatatggctg aggagcttaa cgcgctcaaa actgagcagt
    10381 gctcaaaact gagcagtcat atcggggtga ctgaagatgt ctatctaaac gcgcagggga
    10441 aacagcagcc tctgtaccgt ctcgaccgta agcatacttt cattctggtc gctggctatt
    10501 ccgttcacct ccgcgccaag tgttacgacc atattcagac gttagagcgt cgagtccttc
    10561 aactggaaga ccagaagaag cgagcagcca ttcagtccgc taaccgccga ggcgtgactt
    10621 ggggcgacta ctgcaaaacc tatggtctac ccgctcagaa gctaatgacc gcacttcttc
    10681 agcatcgtgg tctctttcgt aagaacccaa tcagtaacga gtggtctgta aatccaaagt
    10741 attccgactg cttccgtatc attaagccgt cagaccagaa gttctcagca ggtggttata
    10801 actttcgctt caacgctaag ggattggagg tcttcggtaa gcctgcgata gtcgataaac
    10861 tgcgaggcat ccttattgcc ttcactggca ccgaccagca gaagcaagag catctcctga
    10921 agcaagccca atccggcaag ctggagggac tctaagatga ttgacccgcg cacacctatt
    10981 ggtcgtgcaa ctctccgcta ccgtggtctg cctacccgtc acctcctgtc cctgctgaga
    11041 cttggagtcg ataaccctga tcgcccttac tacagtcgag acgagctgat agcgatgctg
    11101 gtcgaccgtg atttgaataa tcagttacgt cgagcctttg cgaaactgga gtcttaacat
    11161 ctccggaaat ttccagacct taaccaacct tatttaaaac cttttgagat gaccgatgcg
    11221 gggagacccg taggcattac tgtgtctgcg ataccgatag tctcaactct caagaggagt
    11281 aattactatg tctag
//
LOCUS       DAZFRJ010000144        11227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000144 DAZFRJ010000000
VERSION     DAZFRJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.145"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(73..1902)
                     /gene="glmS"
                     /locus_tag="ACHKUN_003485"
     CDS             complement(73..1902)
                     /gene="glmS"
                     /locus_tag="ACHKUN_003485"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO6661682.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2064..3434)
                     /gene="glmU"
                     /locus_tag="ACHKUN_003486"
     CDS             complement(2064..3434)
                     /gene="glmU"
                     /locus_tag="ACHKUN_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO6661683.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3785..4204)
                     /gene="atpC"
                     /locus_tag="ACHKUN_003487"
     CDS             complement(3785..4204)
                     /gene="atpC"
                     /locus_tag="ACHKUN_003487"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO6661684.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4225..5607)
                     /gene="atpD"
                     /locus_tag="ACHKUN_003488"
     CDS             complement(4225..5607)
                     /gene="atpD"
                     /locus_tag="ACHKUN_003488"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO6661685.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5634..6497)
                     /gene="atpG"
                     /locus_tag="ACHKUN_003489"
     CDS             complement(5634..6497)
                     /gene="atpG"
                     /locus_tag="ACHKUN_003489"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO6661686.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6548..8089)
                     /gene="atpA"
                     /locus_tag="ACHKUN_003490"
     CDS             complement(6548..8089)
                     /gene="atpA"
                     /locus_tag="ACHKUN_003490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO6661687.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLVDVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8102..8635)
                     /gene="atpH"
                     /locus_tag="ACHKUN_003491"
     CDS             complement(8102..8635)
                     /gene="atpH"
                     /locus_tag="ACHKUN_003491"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO6661688.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8650..9120)
                     /gene="atpF"
                     /locus_tag="ACHKUN_003492"
     CDS             complement(8650..9120)
                     /gene="atpF"
                     /locus_tag="ACHKUN_003492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO6661689.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9182..9421)
                     /gene="atpE"
                     /locus_tag="ACHKUN_003493"
     CDS             complement(9182..9421)
                     /gene="atpE"
                     /locus_tag="ACHKUN_003493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO6661690.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9468..10283)
                     /gene="atpB"
                     /locus_tag="ACHKUN_003494"
     CDS             complement(9468..10283)
                     /gene="atpB"
                     /locus_tag="ACHKUN_003494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO6661691.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10292..10672)
                     /gene="atpI"
                     /locus_tag="ACHKUN_003495"
     CDS             complement(10292..10672)
                     /gene="atpI"
                     /locus_tag="ACHKUN_003495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO6661692.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2699 a         3048 c         2761 g         2719 t
ORIGIN      
        1 tagatgaatt tattgatgtc agttataaaa aagggccacc ggtcggcagc ccttttttat
       61 ctatgatagt gattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtcaacgtc
      121 ggtgcctttg atcagcgcga catggtaagc cagcagctgc agcggaacgg tgtagaagat
      181 cggtgcaatc acctcttcca catgcggcat ctcgatgatg tgcatgttat cgctacttac
      241 aaaacccgca tcctgatcgg cgaagacata caactgaccg ccacgcgcgc gaacttcttc
      301 aatgttggat ttcagttttt ccaacagttc gttgttcggt gcaacgacga taaccggcat
      361 atccgcgtca atcagcgcca gcgggccgtg tttcagctcg cctgcagcgt aggcttcagc
      421 gtgaatgtaa gagatctctt tcagcttcaa tgcgccttcc agcgcgattg ggtactgatc
      481 gccacggccc aggaacagcg cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat
      541 gcgtttgtcc tgagacagca tctgctcaat acggctcggc agcgcctgca gaccatgcac
      601 aatgtcatgt tcaatggagg catccagacc tttcaggcga gacagcttcg ccaccagcat
      661 caacagcaca gttaactgag tggtgaatgc tttagtggat gccacgccga tttccgtacc
      721 cgcgttggtc atcagcgcca gatcggattc gcgcaccaga gaagaacccg gaacgttaca
      781 gattgccagc gaaccaaggt aacccagctc tttcgacaga cgcaggcccg ccagggtatc
      841 cgcggtttcg ccagactgtg acaaggtgat catcaggctg ttacgacgca cggcagattt
      901 gcgatagcgg aattcagagg cgatttcgac gtcgcacgga atacctgcca gcgattcaaa
      961 ccagtaacgg gaaaccatac cggagttata agaagtacca caggcgagga tctgaatatg
     1021 ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc
     1081 gtggctgatg cgcccggtaa gggtgttttt gatcgcgttc ggctgttcgt agatctcttt
     1141 ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca tattgcagat tggattcgat
     1201 atcctgacgt tttacttccg cgccagtttt atcgaagatg tttaccgaac ggcgagtaat
     1261 ttccgcaata tcgccctctt caaggaagat aaagcgacgg gtcaccggca gcagcgccag
     1321 ctggtcagaa gcgataaagt tttcgcccat acccagacca atcaccagcg gactaccaga
     1381 acgtgccgcc agcagggtat ccgggtgacg ggagtccatg atcactgtac cgtacgcacc
     1441 acgcagttgc gggatagcac gcagaacagc ctcacgcaga gtaccgccct gtttcagctc
     1501 ccagttcacc agatgggcaa ttacttcggt gtctgtttca gaaacgaagg tatagccacg
     1561 cgcttttaac tcttcgcgca gcggttcatg gttttcgatg atgccgttat gcaccaccac
     1621 aatgtgttca gaaacatgcg gatgcgcatt cacttctgaa ggttcaccgt gggtcgccca
     1681 gcgagtatga gcaatacccg taccgccatg cagaggatgt tcttccgctg cctgagccag
     1741 catctggact ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat caacaacggc
     1801 cagaccggca gagtcatatc cgcggtattc cagacgacgt aaaccttcaa gaaggatttc
     1861 tgctacatca cgttgcgcga tcgcgccaac aattccacac atagtttttg attccgattt
     1921 atatcgttgt cggtcaacct gtatgcccgt tttttcgggc gccccgagcc ttgtagagag
     1981 tggggttatt tttatagtta ctgcttgtgg gagggagatt atgttatctc ctcatcccat
     2041 gtgaccggat tacccggcca gaatcacttt ttctttaccg gacgacgcca gccttctttc
     2101 tgagtctgcg gcacacggct gatagctaat gcattttcgc cgacattacg cgtcacagtt
     2161 gtacccgcag caatggtcgc gcctttgcct actgttaccg gggccaccag ctgagtgtcg
     2221 gaaccgacaa acacatcgtc gccgataatg gtcttaaatt tattcgcacc atcgtagttg
     2281 caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa
     2341 gtcagatgac cagctttcga gcctttaccc agacgcgctt ttttcatctc aacaaagtta
     2401 ccgacgtgtg caccttccag caactcagca ccaggacgca gtcgggcaaa cgggccaata
     2461 gtacaggccg ctgccagatt cgcgtcttcc acaacggtat acggactgat ttcgcaatca
     2521 tcaccaatca cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga
     2581 gtcacgttgc cctcgatgat aacgttagta tcaatttcaa catcgcgccc gtgagtaagc
     2641 gtaccgcgca aatcaaaacg cgccggatcg cgcagcataa cgcctgctaa cagcagtttt
     2701 tcagcctgtt cggactggta aacgcgctcc agacgggaga gttgcaggcg gttattcaca
     2761 ccttctactt cgcttaaacg ttgcggatga acggcgacga tttcacgccc ttcctgatac
     2821 gccagcgcaa taatgtcggt gatgtagtat tcgccctgag cattattgtt ggtcagcttc
     2881 gccagccagc gtttcatatc tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga
     2941 atctgacgct gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac tttgccattt
     3001 tcacgggtga tacgtccata accggtcgga tcatccagtt tcaccgtcag cagaccaatg
     3061 ccaccctgcg gtttagcatc acgcaggcgc tggagtgttt cgacagagat cagcggcacg
     3121 tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt
     3181 gcatgacccg tacccagctg ctctgcctga agcacccagt taaggttgtc gtctttcagc
     3241 gcctgtttaa gcagatcgcc gccgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat
     3301 tcattcgcag catcaatgac atgctgaacc atcgctttcc cggcaagggt atgcagcact
     3361 ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata
     3421 gcattattca acatacgcgt cctgactgta atttgagaac gaatttaaac cgcttcacct
     3481 tgaaaaaact acattttttt catcgtgaaa tggacagagg ataaattgtt caataacgga
     3541 ttatccccgc tgacgaaagt gccattttcg accatcgtca cgccttttgt ctgcagaaaa
     3601 ttaaggcagt accttcattt aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg
     3661 aataaacggg aaaacagggg tgttttatat cagcaggatc tatgtgaacg ctcttcagga
     3721 cggatcacgc gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc ttttcaagcc
     3781 ggtgttacat cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc gctttggcca
     3841 gttccgcaga cgcctgagcg taatctacgt cgccgtgaga gctgctaatg tgctcttcag
     3901 ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt
     3961 cggccagaac ggtcacgttg ccaggctgca cttcaagaat gccgccagac agatagataa
     4021 actcttcgtg accgtgctgt ttcacgatgc gaatcatacc aggcttaatg gcggtgagca
     4081 gcggtgcgtg gccagggtag atccccagtt caccttcgct acccgttacc tggattttct
     4141 cgaccagacc agagaacatt tgttgctctg cgctgacgac gtccaggtgg taagtcattg
     4201 ccatatcacc ctccgattaa ggcgttaaag ttttttggct ttttccacag cttcttcgat
     4261 ggaaccgacc atgtagaacg cctgctccgg caggtgatcg tattcgcctt ccatgatgcc
     4321 tttaaagcca cggatggtgt ctttcaggga gacgtattta cccggagaac cggtgaatac
     4381 ttctgccacg aagaacggct gggacaggaa gcgctggatc ttacgagcac gcgctaccac
     4441 cagtttgtct tcttcagaca gttcatccat acccaggatg gcgatgatgt ctttcagttc
     4501 ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac
     4561 caccagcggg tccagctgac ggctggtgga gtccagcggg tcaacggccg ggtagatacc
     4621 cagagacgcg atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc
     4681 cggagacgga tcagtcaagt catccgcagg tacgtatacc gcctgtacgg aggtgataga
     4741 accagttttg gtggaggtga tacgttcctg cagaacgccc atctcttccg ccagggtcgg
     4801 ctgataacct accgctgaag gcatacggcc cagcagtgcg gatacttccg taccggccag
     4861 ggtgtaacga tagatgttgt caacgaacag cagaacgtca cgaccttcgt cacggaattt
     4921 ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg gctcgttcat
     4981 ctggccatac accagggata ctttgtcgat aacgttggag tcggtcattt cgtggtagaa
     5041 gtcgttaccc tcacgagtac gttcacctac gcccgcaaac acagagtaac cggagtgctc
     5101 gatcgcgatg ttacgaatga gctccatcat gtttacggtt ttacctacac ccgcaccacc
     5161 gaacagacca actttaccgc ccttagcgaa cggacacatc aggtcgataa ctttgatacc
     5221 ggtttccagc agttcctgag agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat
     5281 cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg accggttcac ccagtacgtt
     5341 catgatacgg cccagagtcg ctttacctac cgggacttcg atcgggtgtt cgaggtcttt
     5401 tacatccaga ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac gcacgatacc
     5461 gccgccgagc tgctgctgaa cttccagcac cagacgctca ttaccatttt gcacctcaag
     5521 agcatcgtac acgcgcggta cggcatcctg agggaattcg acgtcaacta cggcgccgat
     5581 tacctggaca atctttccag tagccatctt aaatcctcta cgaaataacc tgtttaaacc
     5641 gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg acgagctttg
     5701 ttgtatacca actgcagctc tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc
     5761 gccaccatac gggcggcctg ctcgctggcc aggttttcaa ccacgccctg ataaacctga
     5821 gattcgacat aacgacgcag cagggtatcc agcaacgcct tcggatcggg ttcgtacagg
     5881 taatcccagg atttatgttt cagatcatca tcatctgatg ccggtaacgg cagcagctgg
     5941 ctgatggtcg gaacctgaga catggtgtta ataaatttgt tgctgacaat gtaaagcttg
     6001 tccagacggc cttcgtcgta ggcctgcaac atcactttta ccggaccgat cagttcggac
     6061 agggaagggt tatcccccat gccggtgacc tgggcaacaa cattgccgcc cacggagttg
     6121 aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc attgaacgcc tttgtcggtc
     6181 caggtcttca tttccgccag cagttttttg aacaggttaa tgttcaaacc accgcacaaa
     6241 ccacggtcgg tcgacaccac caggtagccc acgcgtttaa cgtcgcgttc ttccaggtat
     6301 gggtgcttat attccagatt accgctcgca aggtgaccaa tcactttgcg catggtatct
     6361 gcataaggac ggctggccgc catgcgatcc tgcgatttac gcattttgga agcggcgacc
     6421 atctccatcg ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt actacgtatc
     6481 tcttttgcgc cggccatgag cttctcctca atgccttgcg gcctgcccta aggcaagccg
     6541 ccagacgtta ccaggattgg gttgctttga aggaatcgag gatgcctttc agcttgcctt
     6601 cgatttcgtc gttgtagcca ccggtctggt tgatctcttg catcaacgga gcgtgatcac
     6661 ggtcgacgta agccagcaga gcggcttcga agctgccgat tttcgacagt tcaacatcca
     6721 ccaggtaacc acgttctgct gcgaacagaa ccagagactg ttgcgctacg gacatcggcg
     6781 catactgttt ctgtttcagc agttcggtca ctttctgacc gtggtcaagc tgcttacgtg
     6841 ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc cagttcacga tactgtgcca
     6901 gagcggtacg gataccaccg gacagttttt tcatgatctt ggtctgtgct gcaccaccaa
     6961 cacgggatac ggaaataccc gggttaaccg caggacgaat accggcgttg aacaggttgg
     7021 tttccaggaa gatctgacca tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa
     7081 cgtcacccgc ctgagtttca ataatcggca gtgcggtcag agaaccggtt ttccctttca
     7141 cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac acgcgcagca cgctccagca
     7201 gacgagagtg gaggtagaat acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga
     7261 gcagcaggga gatctgacgg taagcaacag cctgtttaga caggtcatcg taaatgatca
     7321 gcgcatcttc accgcggtca cggaagtatt cgcccattgc gcaaccggca tacggagcca
     7381 gatattgcag tgcagcggat tcagacgcgg ttgctaccac aacgatggtg ttagccagtg
     7441 cgccgtgctc ttccagttta cgtaccacgt tagaaatggt ggacgctttc tggccgatag
     7501 cgacatagat acatttgata ccggaatcgc gctggttgat gatggcatcg atagccagtg
     7561 cggttttacc ggtctgacgg tcaccgatga tcaattcacg ctgaccacga ccaattggga
     7621 tcatggagtc aacggcttta taaccggtct gtaccggctg atctacggac tgacgttcga
     7681 taacgcccgg agcgattgct tctacagcag agaagccgtc gtgatccagc ggacctttac
     7741 cgtcgattgg tgcacccaga gtgttaacca cacggcccag caggccacgg ccaaccggaa
     7801 cttccaggat acggccagta cacttaactt tcatgccttc ggcaaggtca gcgtacggac
     7861 ccataacaac cgcacctaca gagtcgcgct cgaggttcag tgcgatagcg taacggttac
     7921 ccggcaggga gatcatttca ccctgcatac aatcggccag gccgtgaatg cggataacac
     7981 cgtcacttac agaaacaata gtaccttcgt tgtgagcttc actcacaaca ttgaactgag
     8041 caatgcgctg cttgatcagt tcgctgattt cggtggaatt cagttgcatg ctccagtccc
     8101 cttaagactg caagacgtct gcaaggcgct caagacgacc gcgtacgctg ccatcaatga
     8161 ccatatcacc cgctcggatg ataacgcctg ccattacaga cttatcgatt ttgcaattca
     8221 gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg agctgttgtt
     8281 cactcagtgc ggcagcggaa atgacgtcta cctcagcggt agcctcactc acggcacgaa
     8341 ggtgaataaa ctgctccaga acatccggga gcgcgttaag acgaccattt tcagccatta
     8401 cccgaatcag gttctgaccg ttttcgtcca gttgctcacc acaaactgcg ataaacgact
     8461 cggcgagcgt ttctggcgca agcgcgccag agagaagctc tgccatttgt tcgtttttgg
     8521 ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc tacactttgg tgttcgacgg
     8581 caaagtcaaa agctgctttg gcgtaggggc gagctaccgt aataaattca gacatcagcc
     8641 cctccctcct tacagttcag cgacaagttt atccacgatg tcgctgttag cagcttcatc
     8701 cacggaacgt tcgatgatct tctcggcgcc agcaacagcc aggatagcaa cttgcttacg
     8761 cagctcttca cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct gggccacgat
     8821 tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt
     8881 gttcgcctgc tcgatgatta cctgggcttc cgctttcgct tttttcagct ggtcggtcgc
     8941 gctggccttt gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc
     9001 aatttctttt tgacgttttt cgatggctgc cattaatggc ggccatacgt acttcatgca
     9061 gaacagaacg aacaggacaa acgcgatggc ctggccgagg attgttgcgt taagattcac
     9121 agcacaatgc ctctatttag ttaacgttct gatattgctc tttaaataaa agcaacgctt
     9181 actacgcgac agcgaacatc acgtacagac ccagacctac agcgatcatc gggatagcat
     9241 ccaccagacc cataacgata aagaactgag tacgcagcag aggaatcaga tcaggttgac
     9301 gcgctgcgcc ttccaggaat ttacccccga ggatgccgat accgatcgca gcaccgattg
     9361 ccgccagacc catcatcaca gcggcagcca tgtacagcag atccatattc aggttttcca
     9421 tgacagtctc cagtttgttt cagttaaaac gtagtagtgt tggtaaatta atgttcttca
     9481 gacgccatcg acagatagac gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg
     9541 atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc actgtgacca ccacggcaac
     9601 agaccagcaa tcagaatgaa aatcagctca ccggcataca tgttaccgaa cagtcgcaaa
     9661 ccaagtgaaa ctggtttgga cagcaggctt accccttcaa ggattaagtt gacaggaatg
     9721 aacgcccagt gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc
     9781 attttgatgc tgtagaacag aatcaggata aatacgccca atgccataga cagcgttacg
     9841 ttcacgtccg cagacggaac cacacgcagt gcaggcagac ccagtacatg ttcagcaatg
     9901 tacggcagca ggtcgatagg cagtaaatcc atcaggttca tcaggaatac ccagacgaag
     9961 atcgtcaggg ccagcggagc aatcagcttg cttttgccat ggtacatgtc tttcacgcta
    10021 ccattaacaa agccgatcac cagctcaatc gcggtctgaa acttacctgg cacaccgctg
    10081 gtcgcctttt tggctacgct acggaataaa accaggaaca acagacccag caccaccgag
    10141 aagaacatgg agtcaatatt gattgtccag aaggtggctg gggggttttg tggatccacc
    10201 agcgagaatg tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc
    10261 ggcgtcatat tttctgaagc catgatgcct tttacccttt gttgttaatt acagccggtg
    10321 ccagtatctg aaccaccagc accaaaaccc acgtaacgat cagcggcaag aataccgcct
    10381 ttaaaaccgc caacgccacc accagtaaca ccaacatcgc cagaactttg aaagcttcgc
    10441 caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt atgcgcctgg tgacgccagg
    10501 caaatatcat aaacaaaacg ttaggcagaa agactgccag gcccccgctt attgcagaga
    10561 cgccccagaa ggggtctttg aggctgaaca gcaatccact tgctatcacc accagtaact
    10621 gaacgagcag aagcttccga gcaacgtttc gactcacgag cgacacagac atcacgtttt
    10681 tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa aactttcttt aaggcttaga
    10741 gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc cgtgatttca aacaataagt
    10801 agccaaaagg tgaataaatg tttaaatatt tttccagtgc atacaattgc gacttttctg
    10861 ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta aacactggtg ataaagcgtg
    10921 cttcagatca catattgcgc atgttcgcgc acagcatatt tatttacttg gcaaatgatg
    10981 cctttgcaag tttatgatat ttcagtctaa aaacagatac tgttttaata aatgacattt
    11041 acacaacaaa aaccacccat tgacattttt aataatgttt taacagccaa tgatggttct
    11101 tagcgccgat ttttagcaga ctgatatttt cactaatgac ttattttctg cttaccaaaa
    11161 aaagccacgt tatcttgttg atgcaaaaga gtgaacgtgg cgttaaatgt aaccagttat
    11221 atcagta
//
LOCUS       DAZFRJ010000145        11199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000145 DAZFRJ010000000
VERSION     DAZFRJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.146"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..523)
                     /locus_tag="ACHKUN_003496"
     CDS             complement(<1..523)
                     /locus_tag="ACHKUN_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6661693.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTN"
     gene            879..1454
                     /gene="ssuE"
                     /locus_tag="ACHKUN_003497"
     CDS             879..1454
                     /gene="ssuE"
                     /locus_tag="ACHKUN_003497"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO6661694.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            1447..2406
                     /gene="ssuA"
                     /locus_tag="ACHKUN_003498"
     CDS             1447..2406
                     /gene="ssuA"
                     /locus_tag="ACHKUN_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO6661695.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2403..3548
                     /gene="ssuD"
                     /locus_tag="ACHKUN_003499"
     CDS             2403..3548
                     /gene="ssuD"
                     /locus_tag="ACHKUN_003499"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO6661696.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            3559..4350
                     /gene="ssuC"
                     /locus_tag="ACHKUN_003500"
     CDS             3559..4350
                     /gene="ssuC"
                     /locus_tag="ACHKUN_003500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO6661697.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4347..5114
                     /gene="ssuB"
                     /locus_tag="ACHKUN_003501"
     CDS             4347..5114
                     /gene="ssuB"
                     /locus_tag="ACHKUN_003501"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6661698.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(5321..7933)
                     /gene="pepN"
                     /locus_tag="ACHKUN_003502"
     CDS             complement(5321..7933)
                     /gene="pepN"
                     /locus_tag="ACHKUN_003502"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO6661699.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8199..9401
                     /gene="pncB"
                     /locus_tag="ACHKUN_003503"
     CDS             8199..9401
                     /gene="pncB"
                     /locus_tag="ACHKUN_003503"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO6661700.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9570..10970
                     /gene="asnS"
                     /locus_tag="ACHKUN_003504"
     CDS             9570..10970
                     /gene="asnS"
                     /locus_tag="ACHKUN_003504"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO6661701.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2590 a         2859 c         2932 g         2818 t
ORIGIN      
        1 aatttgttac cgcattcaca ttaccagaag tcgctttccc cgtagcgata taatcaactt
       61 taaaggtcac tgtttgctca gcagaatccg ctaactgaat attcgacgtt tcggtaccgg
      121 gagttaccga tgtagaacca tctggcagat acatttgtag agcaacattc gtcgctgcat
      181 cggtggtagc ctggttggca aatgctgtag gttgcacgcc gtcagccgta ccactaaagg
      241 taaacgtcgc atttttagtt acagtaacat cacagccttc taaggcaatt ggtacatcag
      301 ttttaacgcc agagactgct ccattagttt gcaacttggt ggcgctgaca tttggcaatg
      361 tgactactga atctttcttc gcagctacca gggtacaagc tggagcaata actttaccat
      421 taaatgttac tttgccatcc gtgatagcat tatcatcagc tgccataacg ctggacgttg
      481 cacataccac agttataaac gccgtcaata cacttttttt catcgtcatc atcctgatct
      541 cttcgtaaat atgataatgc ctttaaatga cattagactt aataataaca ataattgacc
      601 gccatttaaa tcaagcatac taaatccaat aagtgacaaa tctttaattt atagatttca
      661 tgaatattta aatattaact gaacgagtaa ttcattgatt caacatctaa tgactatcca
      721 acaaataaat atgcgtgata tataaagccc tttctttagt ttattttcag aaaaagatac
      781 acaaaattta tatttggcat tttcttgtct ctccgacaga ctgaccgaat tgttatcaat
      841 gttaacaaaa aaagaacaat tggttataag gagagagtat gcgtgtcatc accctggcgg
      901 gtagtcctcg ctttccttct cgctccagct ccttgctgga atatgcgcgg gaaaaactaa
      961 attgcctgga tgtagaggtt tatcactgga atctgcaaaa cttcgccccg gaagatctgc
     1021 tttacgctcg tttcgatagt ccggcactca agaccttcac cgaacagctg caacaggctg
     1081 atggactgat agtcgctaca cctgtgtata aagccgccta ttccggcgcg ctgaaaaccc
     1141 tgctcgacct gctgccagag cgcgctttgc aaggcaaagt ggtgctaccg ctggcgacgg
     1201 gcggtacggt ggctcatctg ctggcggtcg attatgccct taagccagtt ttaagtgcga
     1261 tgaaagccca ggagatcctg cacggcgtgt ttgccgatga ctcacaagtt attgattacc
     1321 atcacaaacc ccagttcacg ccaaatctgc aaacccgtct tgataccgcg ctagaaactt
     1381 tctggcaggc attgcaccgc cgcgatgttc aggttcctga ccttctgtct ctgcgaggta
     1441 atgtccatgc gtaacatcat taaactggcg ctggcgggat tgcttagcgt ctctactttt
     1501 gcggttgctg cagaatcctc gcctgaagcg ttacgtatag gctatcagaa aggcagtatt
     1561 ggtatggtat tggcaaaaag ccaccagtta ctggaaaaac gctatccgca atcaaaaatc
     1621 tcctgggtgg agttccccgc gggtccgcaa atgttggaag cgttaaacgt tggcagtatt
     1681 gatctcggca gtaccgggga tattccgcca atctttgccc aggctgccgg ggctgatttg
     1741 gtatatgtgg gcgtcgagcc accgaagccc aaagccgaag tgattctggt ggcagaaaac
     1801 agcccgatca aaaccgtagc cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc
     1861 agttcacaca accttttact gcgtgcactg cgtcaggccg gacttaagtt taccgatatc
     1921 caacccactt acctgacgcc cgctgatgcc cgcgccgcgt tccagcaagg taacgttgac
     1981 gcctgggcta tctgggatcc ctactactct gctgcattat tacagggcgc cgtgcgggtg
     2041 ctgaaagacg gcaccgatct caatcaaact ggatcgtttt atctggcagc tcgtccctat
     2101 gcagaaaaaa acggcgcttt tattcagggc gtactcgcaa cctttagtga ggccgatgcg
     2161 ttaacccgca gccagcgcga gcaaagcatc gctttactgg caaaaacgat gggcttaccg
     2221 gcaccggtga ttgcctctta cttagatcat cgccctccta ccaccatcaa accggttaac
     2281 gccaaggttg ccgccttaca gcagcaaacg gcagatctgt tttatgaaaa tcgtctggtg
     2341 ccgaaaaaag tcgatattcg ccagcgcatc tggcagccca ctcaactgga aggaaaacaa
     2401 ttatgagtct gaatatgttc tggtttttac cgacccatgg tgacgggcat tatcttggaa
     2461 cggaagaagg ttcacgcccg gttgatcacg gctatctgca acaaattgcg caagcggcgg
     2521 atcgtctggg ctataccggt gtgctgatcc caacggggcg atcgtgtgaa gatgcgtggc
     2581 tggtggcggc gtcgatgatc ccggtgacgc agcggctgaa gtttcttgtc gccctgcgcc
     2641 ccagcgtaac ctcacctacc gttgccgctc gccaggccgc cacgcttgac cgtctctcaa
     2701 atggtcgtgc gttgtttaat ctggtcacag gcagcgatcc acaagagctg gcaggcgacg
     2761 gcgtgttcct tgatcatagc gagcgctacg aagcctcggc ggaatttacc caggtctggc
     2821 ggcgcttatt gctgggggaa accgtcaatt tcaacggtaa acatattcat gtgcgcggag
     2881 caaaactgct cttcccgccg attcaacagc cttatcctcc gctttacttt ggcggatcgt
     2941 cagacgttgc ccaggagctg gcggcagaac aagttgatct ctacctcacc tggggcgaac
     3001 cgccagaact agtaaaagag aagatcgaac aagtgcgggc gaaagctgcc gcgcatggac
     3061 gcaaaattcg tttcggtatt cgtctgcatg tgattgttcg tgaaactaac gacgaagcgt
     3121 ggcaggccgc cgagcggtta atctcgcatc ttgatgatga aactatcgcc aaagcacagg
     3181 ccgcattcgc ccggacggat tccgtaggac aacagcgaat ggcggcgtta cataacggca
     3241 agcgcgacaa tctggagatc agccccaatt tatgggcggg cgttggctta gtgcgcggcg
     3301 gtgccggaac ggcgctggtg ggcgatggtc ctacggtcgc tgcgcgaatc aacgaatacg
     3361 ccgcgcttgg catcgacagc tttgtgcttt cgggctatcc gcatctggaa gaagcgtatc
     3421 gggtcggcga gttgctgttc ccacatctgg atgtcgccat tccggaaatt ccccagccgc
     3481 agccgctgaa tccgcaaggc gaagcggtgg cgaatgattt tatcccccgt aaagtcgcgc
     3541 aaagctaagg aagaaataat ggcaacgcca gtgaagaagt ggttattgcg cgttgccccc
     3601 tggtttttac cggtgggcat cgtggcggtg tggcaactgg cctcctcagt tggctggctg
     3661 tcgacgcgta ttttgccctc accggaaggg gtggtgacgg cgttctggac gctctccgcc
     3721 agcggcgaac tgtggcagca tctggcgatc agttcctggc gggcgctgat tggcttttca
     3781 attggcggat cgctggggtt gattttggga ctgattagcg ggctatctcg ctggggagaa
     3841 cggctgctgg atacctcaat tcagatgttg cgcaacgtac cgcatctggc gctgatcccg
     3901 ctggtgattt tgtggtttgg cattgatgaa tccgcaaaaa tctttctggt ggcgctcggc
     3961 acgctattcc ccatttatat caacacctgg catggcatcc gtaatatcga tcgcggtctg
     4021 gtggaaatgg cgcgtagcta tggattatcc ggcataccgc tgtttatcca tgtgatcctg
     4081 cctggtgccc tgccctcaat tatggtcggc gtgcgttttg cgttgggcct gatgtggctg
     4141 acgctgattg ttgccgaaac catttctgcc aattcaggca ttggttatct ggcgatgaat
     4201 gcgcgggagt ttttgcaaac ggacgtggtg gtagtcgcta ttattcttta cgccctgctc
     4261 ggcaaacttg ctgacgtcag cgcgcagctg ttagagcgcc tctggttgcg ctggaacccg
     4321 gcttatcatt tgaaggaggc cacggtatga atactgctcg tctgaaccag ggcacaccat
     4381 tgttgctcaa tgcagtaagc aaacattacg cggaaaatat cgtcctgaac caactggatt
     4441 tacatattcc ggcaggtcag tttgtggcgg tggtgggccg cagcggtggt ggcaaaagta
     4501 ccctgctgcg cctgctggca ggtctggaaa cgccaaccgc aggcgatgtg ttagcgggca
     4561 ccacaccgct ggctgagatt caagaagaca cgcgaatgat gtttcaggat gcgcgtctgc
     4621 tgccgtggaa atcggtgatt gataacgttg ggttaggcct taaaggccag tggcgcgatg
     4681 ccgcgcgtcg ggcgttggct gcagtaggac tggagaatcg cgccggggaa tggcctgccg
     4741 cactttctgg cgggcagaaa cagcgtgtgg cgctggcaag agcgttgatt catcgaccgg
     4801 gtttattgtt gcttgatgaa ccgctcgggg cgctggacgc cttaacgcga ctcgagatgc
     4861 aggatttgat tgtgtcactt tggcagcagc acggctttac cgtactgttg gtgacgcatg
     4921 atgtgagcga agcggtagca atggctgacc gggtgttgtt aattgaagag ggaaaaattg
     4981 gtctggattt gacagtggat atcccccgtc cgcgtcgatt agggtcggtg cgattagcgg
     5041 agctggaagc ggaagtattg caacgggtaa tgcggcgtgg gcattctgaa caactgattc
     5101 ggcgtcatgg ttaatatgcc ggatgtggcg cacaaaacca gcattttcaa cggactagta
     5161 ggcctgataa gacacgtcaa gcatcgcatc aggcagttgg gcacggctgc cggatgcggc
     5221 gtgaacgcct tatccagcct acaaaaccag caatttcaac ggagttgtag gcctgataag
     5281 acgcgtcaag cgtcgcatca ggcattttgg ctcaaaagta tcaagccagt gctttggtga
     5341 tcttctcgta cagatcgcca gagagatttt ccagcccttt caactgttcc agcgccgcgc
     5401 gcattttctc ctgacgtttg gcatcataac gtttcaggcg aatcagcggt tcaatcagac
     5461 gtgaagctac ctgcgggtta cggctgttga ggtcggtaag catttccacc aggaactggt
     5521 aaccgctgcc atcttcggca tggaacgctg ccgggttgct gcccgcaaac gcaccaatca
     5581 acgaacgaat acggttcggg ttgctcatgg taaatgagcg atgctgcaac agaccacgca
     5641 ccgtctccag cacattcgcc gccgggctgg tggcttgcag gataaaccat ttatccatca
     5701 ccagaccgtc ctgatgccac ttatcgtcgt actcctgcat cagcgcgtca cggcaaggca
     5761 gctgtgcagc aaccgccgca gaaagcgctg ccagcgcatc ggtcatattg tttgcttcat
     5821 ggtattgctt gctcaccagc acgtcagcca gatgcgtttc accaaaagca aggaagcgca
     5881 ggcaggcatt acgcagagtg cgttttgcaa tatcttcatg ctcaacacgg tactcgctct
     5941 ggtaattcgc gttgtaaata gccagcagct catccgccag ttcagtcgcc agagtacgag
     6001 tgagtgcttc gcgtacttcg gcaatagcaa tcgggtcgat gatatcgaac agttcagcca
     6061 tttcattgac agaaggcagc gtcaggattt ctgccgccag cgccggatca atcttctcat
     6121 cgagcagcac cgcgcggaaa gcgtcagcca catgtaccgg cagagacagc ggctgccctt
     6181 gctgatgacg ggcgacgttc agcttgatgt aggttgccag caaactttgc gccgcatccc
     6241 aacgggagaa atcattacgc gcatgacgca tcaggaaggt cagttgctga tcgctccact
     6301 tatattccag tttcactggc gcagagaatt cgcacagcag cgcaggcacc ggctggaagt
     6361 agacattatc aaaaacaaag gtctgttccg cctgggtgac gttcagcacg gaattcaccg
     6421 gatgaccgcc tttctgcaac gggatcactt tgccttcgtt gtcatacaat tcgatggcaa
     6481 atggaatatg cagcggctgt ttttctgcct gatccggcgt ggctggcgtg cgctggctga
     6541 tggtcagggt gtactgctcg gtttccggat tgtagtcgtc tttgacggtc acaatcggcg
     6601 tgccggactg gctgtaccaa cggcggaaat gggagagatc gacattcgac gcatcttcca
     6661 tcgcctgcac aaagtcgtcg caggttgccg cgctgccatc atggcgctcg aaatagagtt
     6721 gcatcccttt ctggaagttt tcttcgccca gcagggtgtg gatcatgcga atcacttccg
     6781 cgcccttctc gtaaacggtc agggtgtaga agttgttcat ctcaatgacc atatccgggc
     6841 ggatcgggtg cgccatcggg ctggcgtctt ccgcaaactg caatccgcgc atggtgcgca
     6901 cattattgat acggtttacc gcgcgggaac caaggtcaga gctgaattcc tgatcgcgga
     6961 agacggttaa accttctttc aggctgagct ggaaccagtc gcggcaggtc actcggttac
     7021 cggtccagtt atggaaatat tcatggccga taacgcgttc aatatcgaga taatctttgt
     7081 cggtggcggt gtcggtgcgg gccaacacat atttggagtt aaagatattc agacccttat
     7141 tctccattgc gcccatattg aagaaatcca ccgcgacgat catatagatg tcgaggtcat
     7201 actccaggcc aaagcgttct tcatcccatt tcatggagtt tttcagcgag gtcatcgccc
     7261 acggcgcgcg atcaaggttg ccgcgatcga cgtacagctc cagtgctact tcgcgaccag
     7321 aacgcgtggt aaaggtgtcg cgcagtacat caaagtcgcc tgccaccagc gcaaacaggt
     7381 agcacggttt cgggaacggg tcctgccact gtacccaatg gcgtccgttt tccagttcac
     7441 cttgcgcaac gcggttaccg ttggaaagca ggaagggata tttggtttta tcggcaataa
     7501 ttttggtggt aaaacgcgcc agcacgtccg ggcggtcgag ataatacgta atatggcgga
     7561 aaccttcggc ttcacactgg gtgcaaagcg catcgcctga ctgataaagc ccttccagcg
     7621 ccgtattcgc cgccgggctt atttcattaa tgatcttaag cgtaaaacgc tccggcaaat
     7681 tactgatgac cagtgcgccc tcttcttctt tccaggcggt ccacggctca tcattaatat
     7741 gaacagaaac cagtttgagg tcttcgccgt tgagacgaag cggagcatct gatgcaccat
     7801 gacggacagc ctggctgacc gcggtaacga ccgtcttttg cgcgtcgagg tcaaaggtca
     7861 agtcaatatc agtaatctgg taatccggcg cacgataatc gtgacggtat ttggcttgtg
     7921 gctgttgagt cataaataac ctttagcatc ttttatagag tctggtgttc agtctattcc
     7981 tgttgcgtaa atcgcgctat gcagaatctt catcttttca ggtacaaacg cctttattgc
     8041 tacattttta taacatacac cgcgtaatgc catcgaccag aaaggtggca tatggtgtga
     8101 tcggggttca ataaattgcg aaacaaggta tactccagca gttcctgaag atgtttattg
     8161 tactaaacgc tcctgtacga ggacgctact gcgcacctat gacacaattc gcttctcctg
     8221 ttctgcactc gttgctggat acagatgctt ataagttgca tatgcagcaa gccgtgtttc
     8281 atcactatta cgatgtgcat gtcgcggcgg agtttcgttg ccgaggtgac gatctgctgg
     8341 gtatttatgc cgatgctatt cgtgaacaga ttcaggcgat gcagcacctg cgcctgcagg
     8401 atgatgaata tcagtggctc tctgccctgc ctttctttaa ggccgactat cttaactggt
     8461 tacgcgagtt ccgctttaac ccggaacaag tcaccgtatc caacgataat ggcaagctgg
     8521 atattcgttt aagcggcccg tggcgtgaag tcatcctctg ggaagttcct ttgctggcgg
     8581 ttatcagtga aatggtacat cgctatcgct caccgcaggc cgacgttgcg caagccctcg
     8641 acacgctgga aagcaaatta gtcgacttct cggcgttaac cgccggcctt gatatgtcgc
     8701 gcttccatct tatggatttt ggcacccgtc gccgtttttc tcgcgaagta caagaaacca
     8761 tcgttaagcg tctgcaacag gaatcctggt tcgtgggcac cagcaactac gatctggcgc
     8821 gtcggctttc cctcacgccg atgggaacac aggcacacga atggttccag gcacatcagc
     8881 aaatcagccc ggatctagcc aacagccagc gagctgcact tgctgcctgg ctggaagaat
     8941 atcccgacca acttggcatt gcattaaccg actgcatcac tatggatgct ttcctgcgtg
     9001 atttcggtgt cgagttcgcc agtcgctatc agggcctgcg tcatgactct ggcgacccgg
     9061 ttgaatgggg tgaaaaagcc attgcacatt atgaaaagct gggaattgat ccacagagta
     9121 aaactctggt tttctctgac aatctggatt tacgcaaagc ggttgagcta taccgccact
     9181 tctcttcccg cgtgcaatta agttttggta ttgggactcg cctgacctgc gatatccccc
     9241 aggtaaaacc cctgaatatt gtgattaagt tggttgagtg taacggtaaa ccggtggcga
     9301 aactttctga cagccctggc aaaactatct gccacgataa agcgtttgtt cgggcgctgc
     9361 gcaaagcgtt cgaccttccg catattaaaa aagccagtta atatcatcag ggagctaatc
     9421 ggctcccttt ttttaccttt aattccgaaa tctttcgctg catttgcgaa ttctgcttgt
     9481 ctgattgcag atgccaggta acataggtat ccccccattt acgggtggac aagtgtttat
     9541 tttttccgac tattaacaga gagaatatta tgagcgttgt gcctgtagcc gacgtactcc
     9601 agggccgcgt agccgttgac agcgaagtca ccgtacgcgg atgggtacgt acccgccgag
     9661 attcaaaagc tggcatctcc ttcctcgccg tttatgacgg ttcctgcttt gatcctgtac
     9721 aggctgtcat caataattct ctgcccaatt acaatgaaga cgtcctgcgt ctgaccaccg
     9781 gctgctcggt cattgtgacg ggtaaagtcg tggcgtcgcc gggccagggg caacaatttg
     9841 aaattcaggc cagcaaggtt gaagttgctg gttgggttga agatccagac acttacccga
     9901 tggcggcaaa acgccacagc attgagtatc tgcgtgaagt cgctcacctg cgtccgcgca
     9961 caaacctgat tggtgccgtc gcgcgcgttc gtcatacgct ggcgcaggcg ctgcatcgct
    10021 tctttaacga gcagggattc ttctgggttt caacgccact gattaccgca tctgataccg
    10081 aaggtgcagg cgaaatgttc cgcgtttcta cgctggatct ggaaaacctg ccgcgtaacg
    10141 atcagggcaa agtggatttc gacaaagact tctttggtaa agagtctttc ctgaccgtat
    10201 ctggccagtt gaacggcgaa acctacgctt gcgcattatc caaaatttat accttcggcc
    10261 cgactttccg tgctgaaaac tccaacacca gccgtcacct ggcggaattc tggatgctgg
    10321 agccggaagt ggcgtttgct aacctgaacg atatcgcggg tctggctgaa gccatgctga
    10381 aatatgtctt caaagcggtt ctcgaagaac gcgcagacga catgaaattc ttcgctgaac
    10441 gcgtagataa agatgccgtt tcacgtctgg aacgcttcat tgaggccgat ttcgcgcagg
    10501 tggattacac cgacgcagtg accattctcg aaaactgcgg caggaagttt gaaaacccgg
    10561 tttactgggg cgtcgatctc tcttctgagc atgagcgtta tctggcggaa gaacacttta
    10621 aagcaccggt agtggttaaa aactatccga aagatattaa agcgttctat atgcgcctca
    10681 acgaagacgg taaaaccgtt gcggctatgg acgttctggc tccgggcatc ggtgagatca
    10741 ttggtggctc ccagcgtgaa gaacgtctgg acgttctgga cgagcgtatg ctggaaatgg
    10801 gcctgaataa agaagattac tggtggtatc gcgatctgcg tcgctacggt actgttccgc
    10861 attcaggttt cggtcttggt tttgaacgcc tgattgctta cgtaactggc gtgcaaaacg
    10921 tgcgtgatgt gattccgttc ccacgtactc cgcgcaacgc cagcttctga ttttcttcct
    10981 caaagagcca gcgcctgctg gctctttttt atcctttctg tcaagttatc tgtttgttaa
    11041 gtcaagcaat ctatttgcaa ccccgccata acgatcatcc tgttacggaa tattacattg
    11101 caacatttac gtacaaaaaa taacccgcat tcttattgcg gattagtttt ttcttagctg
    11161 atagcacaat tttcatacta ttttttggca ttctggatg
//
LOCUS       DAZFRJ010000146        11149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000146 DAZFRJ010000000
VERSION     DAZFRJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.148"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..74
                     /locus_tag="ACHKUN_003505"
     CDS             <1..74
                     /locus_tag="ACHKUN_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6661702.1"
                     /translation="MDRITLPSTDTARGKGKWKIITA"
     gene            53..157
                     /gene="speFL"
                     /locus_tag="ACHKUN_003506"
     CDS             53..157
                     /gene="speFL"
                     /locus_tag="ACHKUN_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO6661703.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            468..2666
                     /gene="speF"
                     /locus_tag="ACHKUN_003507"
     CDS             468..2666
                     /gene="speF"
                     /locus_tag="ACHKUN_003507"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO6661704.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2663..3982
                     /gene="potE"
                     /locus_tag="ACHKUN_003508"
     CDS             2663..3982
                     /gene="potE"
                     /locus_tag="ACHKUN_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO6661705.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4038..5678)
                     /gene="pgm"
                     /locus_tag="ACHKUN_003509"
     CDS             complement(4038..5678)
                     /gene="pgm"
                     /locus_tag="ACHKUN_003509"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO6661706.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5704..6249)
                     /gene="seqA"
                     /locus_tag="ACHKUN_003510"
     CDS             complement(5704..6249)
                     /gene="seqA"
                     /locus_tag="ACHKUN_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO6661707.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6434..7198
                     /gene="ybfF"
                     /locus_tag="ACHKUN_003511"
     CDS             6434..7198
                     /gene="ybfF"
                     /locus_tag="ACHKUN_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6661708.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7338..7631
                     /gene="ybfE"
                     /locus_tag="ACHKUN_003512"
     CDS             7338..7631
                     /gene="ybfE"
                     /locus_tag="ACHKUN_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO6661709.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7771..8301
                     /gene="fldA"
                     /locus_tag="ACHKUN_003513"
     CDS             7771..8301
                     /gene="fldA"
                     /locus_tag="ACHKUN_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO6661710.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8511..8597
                     /gene="uof"
                     /locus_tag="ACHKUN_003514"
     CDS             8511..8597
                     /gene="uof"
                     /locus_tag="ACHKUN_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO6661711.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8590..9036
                     /gene="fur"
                     /locus_tag="ACHKUN_003515"
     CDS             8590..9036
                     /gene="fur"
                     /locus_tag="ACHKUN_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO6661712.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9120..9446)
                     /gene="chiQ"
                     /locus_tag="ACHKUN_003516"
     CDS             complement(9120..9446)
                     /gene="chiQ"
                     /locus_tag="ACHKUN_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO6661713.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9496..10902)
                     /gene="chiP"
                     /locus_tag="ACHKUN_003517"
     CDS             complement(9496..10902)
                     /gene="chiP"
                     /locus_tag="ACHKUN_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO6661714.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2700 a         2752 c         2792 g         2905 t
ORIGIN      
        1 tcatggatag aataactcta ccttcaactg acacagcaag aggtaaaggt aaatggaaaa
       61 taataaccgc ttaatgcctc atataaggcg gacaacccat atcatgaagt ttgcccatcg
      121 taatagtttt gactttcatt tctttaatgc ccgctagtct tctgactaaa gggcacccca
      181 acgtacaggt ctccctgact ttaagcatta caggttaata cctgtattcc tcggtgctca
      241 tatactgcta acccttttaa actctaaata attcgagtcg cagcacttgc aacttgaggt
      301 atgacgagta tagccagtta ccgggctggt ctgggttatt gcatctgcaa aaagcaaact
      361 actgatttat ttatcagcgg tggagctttg ctttttttcc ggcgtgatcg atttctcctt
      421 tgagaaattg aggacctgct attacctgaa ataaagagat gaacaaaatg tcagaattaa
      481 aaattgccgt tagtcgttct tgcccggatt gtttttccac tcatcgtgca tgcgtgaata
      541 tagacgaaag taattatatt gacgttgccg ccattatttt atcagtcagt gatgttgaac
      601 gtggaaaact cgatgaaata gacgctactg gctatgacat tcctgttttt attgcaacgg
      661 aaaatgaaga acgtgtaccc gcagaatatc tgccacgtat ttctggtgtc ttcgaacatt
      721 gcgaatcacg taaagagttt tatggacgtc agttagaaac cgctgccagc cattatgaaa
      781 cgcaactgcg cccgcccttc ttccgcgcac tggtcgatta tgttaatcag ggcaatagtg
      841 cttttgactg ccctggtcat caggggggtg agtttttccg tcgccatccg gcaggcaatc
      901 agttcgtgga atactttggc gaaatgctct tccgctctga cttgtgtaat gctgacgtag
      961 caatgggcga tctgctgatt catgaaggtg cgccatgtat tgcacaacag catgcggcta
     1021 aagtctttaa tgccgataaa acctatttcg ttttaaacgg tacttcatct tctaataaag
     1081 tggtcttaaa cgcattgctg acgccgggcg atctggtgtt gtttgaccgc aataaccata
     1141 aatctaacca ccacggtgcg ttgctacagg ctggcgcgac accggtttat ctggaaaccg
     1201 cacgtaaccc gtatggcttt atcggtggca ttgatgcgca ctgttttgaa gaaagttacc
     1261 tgcgtgagct gatcgcggaa gtcgcaccgc agcgggcaaa agaggctcgt cctttccgcc
     1321 tcgctgtgat tcagttaggc acctacgacg gtacgattta taacgcccgc caggtggtgg
     1381 ataaaattgg tcatctgtgt gactacattc tgtttgactc cgcatgggtt ggctatgagc
     1441 aatttatccc gatgatggct gactgttcgc cgctgttgct ggatcttaat gagaacgatc
     1501 cgggtattct ggttacgcaa tccgtacata aacagcaggc tggtttttct caaacttcac
     1561 aaatccataa aaaagacagc cacatcaaag ggcaacagcg ttatgtaccg cacaaacgca
     1621 tgaacaacgc ctttatgatg catgcctcca ccagcccgtt ctatccgctg tttgccgcac
     1681 tggatatcaa cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg atggattgtg
     1741 tggtaaatgg cattaatgcc cgcaagctga tcctcgataa ctgtcagcat attcgtccgt
     1801 tcgtacctga actggtggat ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg
     1861 ttgatctgcg cttcttccag tttgtaccag gtgagcactg gcactctttt gaaggctatg
     1921 cagagaatca atactttgtc gatccatgca aactgttgct gacaacccca ggtattgatg
     1981 cacgtaacgg cgaatatgaa gcgttcggtg tacccgcgac gattcttgct aacttcctgc
     2041 gcgaaaatgg cgtagtgccg gaaaaatgcg atcttaactc catcctcttc ctgctgactc
     2101 cggcagaaga tatggccaag cttcagcaac ttgttgccct gctggtacgc ttcgaaaaac
     2161 tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa cagcatgaag
     2221 agcgctacgc cggttatacc ctgcgtcagt tgtgtcaaga aatgcatgat ttgtatgccc
     2281 gtcacaacgt gaaacaactg caaaaagaga tgttccgtaa ggaacacttc ccacgcgtaa
     2341 gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag ctggttcgtc
     2401 tgccggatgc agaaggccgt atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc
     2461 tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg
     2521 aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag
     2581 aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca
     2641 ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca
     2701 gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa
     2761 gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg taggctcaat
     2821 ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gcggtatggg
     2881 cggttatgcg gagtatgctt tcggtaaatc cggtaacttt atggcgaact atacctacgg
     2941 cgtctcgctg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga
     3001 actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg
     3061 gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac
     3121 cgtgtggggg gtcattattc cggtggtagg cctgtgcatt atcggctggt tctggtttag
     3181 cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcagtagg
     3241 ttcttccatc gctatgacgc tgtgggcttt tctcggtctg gagtctgcgt gtgcgaatac
     3301 tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg
     3361 tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc ctaatatgga
     3421 gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttca caccagaagt
     3481 gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca
     3541 gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt
     3601 ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca
     3661 gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt
     3721 taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat
     3781 tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt
     3841 tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag ccatgctgta
     3901 cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac cacgctttga
     3961 actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgccct
     4021 ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct
     4081 ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat cttatatgcg
     4141 tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg
     4201 ccgatagaag caccattacc cggtgccgcc gtcagacgtg cggtgatcgg gtcacctgcc
     4261 agggtgctgg cgctcaccat ttccggagac agcttagaca acgccgcttt ttgtgcggaa
     4321 gtcgcagcgg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc cagttcgttg
     4381 tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacata
     4441 atgatgccgt ctttatcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagccccg
     4501 gcgctctctt cgccgccaaa gccgaaactg ccgtcgaaca gaccatcaac aaaccatttg
     4561 aatcctaccg ggacttctac cagtttacgg cccaaggcgt tgaccacacg gtcgatcatc
     4621 gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg acgatgctgg
     4681 aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg
     4741 acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc
     4801 agtgccagca ggcccgccat cgcacactcg gaggagcagt ccatacggat cgtgccgtct
     4861 ttatcaaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat ggtcaggttg
     4921 aggttgtaat actcgccaat acgcttccag tattcgatac cggaaccgcc cagcggatca
     4981 acgccaagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagacct
     5041 tccacaaacg gctgcaccag atcctgctct ttcacatgac cggatgccat cgcttcgtcg
     5101 agagagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc cctgtcttcc
     5161 accactttgg tgacgttggt atcagccggg ccaccatttg gcgggttgta cttgatacca
     5221 ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag cgggccacct
     5281 tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa tccattgttt
     5341 tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga aatgaacgca
     5401 ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat gccatttttc
     5461 gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg
     5521 cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct
     5581 gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc
     5641 tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac
     5701 gttttagata gttccgcgaa ccttctcaat caattccgcc gggaattgca tcgactgcat
     5761 gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg
     5821 cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc
     5881 tgccgcaaag taaacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg
     5941 ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg
     6001 ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt
     6061 tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg
     6121 agcagcaggc tgtgatgagg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct
     6181 ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat
     6241 cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccag gagtatatga
     6301 ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt
     6361 atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa
     6421 aaagaacttt gccatgaaat tgaatatccg cgcgcaaact gcacaaaacc agcacaataa
     6481 ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg gcgtactggc
     6541 tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc
     6601 accgagagat ccggtaatga attacccggc gatggcgcag gatcttgttg atactctgga
     6661 tgcacagcag atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat
     6721 ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg atatcgcacc
     6781 ggtcgactat cacgtacgcc gtcatgatga gattttcgcg gctatcaacg cggtcagtga
     6841 atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc ttaatgaaga
     6901 aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc
     6961 ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca
     7021 ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt accgtgatga
     7081 tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg gtcactgggt
     7141 ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca atgattaacg
     7201 caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg gcgtgactca
     7261 tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg attgtttccc
     7321 ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg
     7381 cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gaacagctgc
     7441 gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac
     7501 tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggagtcg cgcaatatga
     7561 gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg
     7621 gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt
     7681 ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat
     7741 ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc
     7801 gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt
     7861 gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta tgacattctg
     7921 ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc
     7981 ccgactctcg aagagattga tttcaacggc aaactggttg cgctgtttgg ttgtggtgac
     8041 caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga catcattgaa
     8101 ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt cgaagcatca
     8161 aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga ccgtcagccg
     8221 gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga gttgcatctc
     8281 gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata
     8341 gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctgtacaa
     8401 tgtcccggtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg tttttattaa
     8461 caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca
     8521 ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta gtaacaggac
     8581 agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt
     8641 ccacgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa
     8701 gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc
     8761 gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga aggcggtaaa
     8821 tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct cgactgcggc
     8881 aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat tgccgcaaaa
     8941 catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat
     9001 tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa
     9061 cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac agctctcact
     9121 tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt caccgcctga
     9181 tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg tacactttcc
     9241 tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag
     9301 gcttcaattt tttctggcga accttgtgcg gtgttgatac aggcgctgta tgcctctttc
     9361 agacgactgt cttcctgtgg cgcggttgat tgcgcacaag ctaccagccc cgatgccatt
     9421 atggcgatga gaatcagttt tttcatagca aattcctcaa acgtgcggca ggcacaaaac
     9481 ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg
     9541 ctcatcctgg aagatgttac cgtaaccgcc gccccagctt gggatgtcag agtggttgtc
     9601 gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta
     9661 gaccgcatcc aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc
     9721 cggattgctc tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat
     9781 ggcgaagcct ggaagattcc agtttttcag gtcatacatc gcaccgaaga agaccgcttt
     9841 ttcgccgttg gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga
     9901 ggaagcgtag gttggagtca tacgttgcag gaagtatccc tgctgaccgt cagccttaac
     9961 ccaggtgcct tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc
    10021 ttgcagccag gcggtgccgt cataaaggtc gttgacgctg cgatcgtcaa ctttatcgcg
    10081 cgtaccatag aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct
    10141 ggctttggca aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac
    10201 gaagttattt ttgaagtcgt atttcgcacc gatggagtgc aggtaatcaa ctttggtggt
    10261 tttatcgttc tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt
    10321 ccacatgtag gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc
    10381 cgccccctga taagtacctg gcataaagct ccagtgcggc gctaacagcg tttgaccagt
    10441 tggctgaatg taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt
    10501 atacaggctt ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt
    10561 tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt
    10621 aaacgcagca atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt
    10681 ggcattccag gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt
    10741 acggtcgcgt tcacgctgcc agtaatatat accgccggtt aaggttgaat cgtcgatgaa
    10801 tccttcggcg cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcgat
    10861 agccagcgcc agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac
    10921 gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa aaattaaaaa agtagcgttg
    10981 agaaaagtct cgctgactac aggaacagac tatttttcgc gacgcgaatt atcaatctct
    11041 ttctgtaacc agaatgtaag aacatgagca accgcacata ttttgatgat ttttgttaca
    11101 aggcttaagt ttgcggttga aaggcttgca gaagaataaa agcgtgaat
//
LOCUS       DAZFRJ010000147        11022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000147 DAZFRJ010000000
VERSION     DAZFRJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.149"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            398..1738
                     /gene="dcuB"
                     /locus_tag="ACHKUN_003518"
     CDS             398..1738
                     /gene="dcuB"
                     /locus_tag="ACHKUN_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO6661715.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1816..3462
                     /gene="fumB"
                     /locus_tag="ACHKUN_003519"
     CDS             1816..3462
                     /gene="fumB"
                     /locus_tag="ACHKUN_003519"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6661716.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3585..4214
                     /gene="yjdF"
                     /locus_tag="ACHKUN_003520"
     CDS             3585..4214
                     /gene="yjdF"
                     /locus_tag="ACHKUN_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO6661717.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4353..5774)
                     /gene="melB"
                     /locus_tag="ACHKUN_003521"
     CDS             complement(4353..5774)
                     /gene="melB"
                     /locus_tag="ACHKUN_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO6661718.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5877..7232)
                     /gene="melA"
                     /locus_tag="ACHKUN_003522"
     CDS             complement(5877..7232)
                     /gene="melA"
                     /locus_tag="ACHKUN_003522"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO6661719.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7515..8423
                     /gene="melR"
                     /locus_tag="ACHKUN_003523"
     CDS             7515..8423
                     /gene="melR"
                     /locus_tag="ACHKUN_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO6661720.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRAMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8622..10889
                     /gene="adiA"
                     /locus_tag="ACHKUN_003524"
     CDS             8622..10889
                     /gene="adiA"
                     /locus_tag="ACHKUN_003524"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO6661721.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2840 a         2912 c         2686 g         2584 t
ORIGIN      
        1 acaaaagtta attaactatt atgtcacccg cattatgtgt atttttaccc acaaatgggt
       61 agatcagatt aatctttaaa cctaatgata tctgccctga gaacaaaaaa tagaccgata
      121 aatatcaata agataacagc aaacaaaaca ttaacatctg cgcagtacaa actataaacc
      181 catcgccaga gagtttttct ctctgaaaaa gccgcttatc acagtgcata aatttgccgc
      241 tgctttaatc agccaatatt tactgtgagg tatttgctaa agccggtaac gaccaaacgg
      301 atatttagtc aggctctgaa aacagttcat acaaaacaga acgtgactgt gatctattca
      361 gcaaaaattt aaataggatt atcgcgaggg ttcacacatg ttatttacta tccaacttat
      421 cataatactg atatgtctgt tttatggtgc cagaaagggt ggtatcgcgc tgggtttatt
      481 aggcggtatc ggtctggtca ttctggtctt cgtcttccac cttcagccag gtaaaccacc
      541 ggttgatgtc atgctggtta tcattgcggt ggtggcggca tcggcgacct tgcaagcttc
      601 gggcggtctt gatgtcatgc tgcaaattgc cgagaagctg ctgcgccgca acccgaaata
      661 tgtctcaatt gtcgcgccgt ttgtgacctg tacactgacc attctttgcg gtacgggtca
      721 tgtggtttac accattctgc cgatcatcta cgacgtcgcc attaagaaca acatccgtcc
      781 ggaacgtccg atggcggcaa gttctatcgg tgcacagatg gggattatcg ccagtccggt
      841 gtcggttgcg gtcgtgtctc tggttgcaat gctgggtaat gtcacctttg atggtcgcca
      901 tcttgagttc ctcgacctgc tggcaatcac cattccatcg acgttaatcg gtatcctggc
      961 gatcggtatc ttcagctggt tccgcggtaa agatctggat aaagacgaag agttccagaa
     1021 attcatctcc gtaccggaaa accgtgagta tgtttacggt gataccgcga cgctgctcga
     1081 taaaaaactg ccgaaaagca actggctggc aatgtggatt ttcctcggtg caatcgctgt
     1141 agtcgccctt cttggtgctg attcggacct gcgtccatcc ttcggcggca aaccgctgtc
     1201 gatggtactg gttattcaga tgtttatgct gctgaccggg gcgctgatta ttatcctgac
     1261 caaaaccaat cccgcgtcta tctcaaaaaa cgaagtcttc cgttccggta tgatcgccat
     1321 cgtggcggtg tacggtatcg catggatggc agaaaccatg ttcggtgcgc atatgtctga
     1381 aattcagggc gtactgggtg aaatggtgaa agagtatccg tgggcgtatg ccattgttct
     1441 gctgctggtt tccaagtttg taaactctca ggctgcggcg ctggcggcga ttgttccggt
     1501 cgcgctggcg atcggcgttg atccggcata catcgtggct tcagcaccgg cttgctacgg
     1561 ttattacatc ctgccgactt atccgagcga tctggcagcg attcagtttg accgttccgg
     1621 caccacccac atcggtcgct tcgtcatcaa ccacagtttt attctgccgg ggttgattgg
     1681 tgtgagcgta tcgtgcgtct tcggctggat cttcgccgcg atgtacgggt tcttataaat
     1741 gcactttgcg tgccgcccgt gactacgcgg cacgccattt tcgaataaca aatacagagt
     1801 tacaggctgg aagctatgtc aaacaaaccc tttatctacc aggcaccttt cccgatgggg
     1861 aaagacaata ccgaatacta tctactcact tccgattacg ttagcgttgc cgacttcgac
     1921 ggcgaaacca tcctgaaagt ggaaccagaa gccctgaccc tactggcgca gcaagccttt
     1981 cacgacgctt ctttcatgct ccgtccggca caccagaaac aggttgcggc tattcttcac
     2041 gatccagaag ccagcgaaaa cgacaagtac gtggcgctgc aattcttaag aaactccgaa
     2101 atcgccgcca aaggcgtgct gccgacctgc caggataccg gcaccgcgat catcgtcggt
     2161 aaaaaaggcc agcgcgtgtg gaccggtggc ggtgatgaag aagcgctgtc gaaaggcgtc
     2221 tataacacct atatcgaaga taacctgcgc tattcacaga atgcggcgct ggacatgtac
     2281 aaagaggtca acaccggcac taacctgcct gcgcaaatcg acctgtacgc ggtagatggc
     2341 gatgagtaca aattcctttg cgttgcgaaa ggcggcggct ctgccaacaa aacgtatctc
     2401 taccaggaaa ccaaagccct gctgactccc ggcaaactga aaaacttcct cgtcgagaaa
     2461 atgcgtaccc tcggtactgc agcctgcccg ccgtaccata tcgcgtttgt gattggcggt
     2521 acgtctgcgg aaaccaacct gaaaaccgtc aagttagcaa gcgctcacta ttacgatgaa
     2581 ctgccgacgg aagggaacga acatggtcag gcgttccgcg atgtccagct ggaacaggaa
     2641 ctgctggaag aggcccagaa actcggtctt ggcgcgcagt ttggcggtaa atacttcgcg
     2701 cacgacattc gcgttatccg tctgccacgt cacggcgcat cctgcccggt cggcatgggc
     2761 gtctcctgct ccgctgaccg taacattaaa gcgaaaatca accgcgaagg tatctggatc
     2821 gaaaaactgg aacacaaccc aggccagtac attccacaag aactgcgcca ggccggtgaa
     2881 ggcgaagcgg tgaaagttga ccttaaccgc ccgatgaaag agatcctcgc ccagctttcg
     2941 caatacccgg tatccactcg tttgtcgctc accggcacca ttatcgtggg ccgagatatt
     3001 gcacacgcca agctgaaaga gctgattgac gccggtaaag aacttccgca gtacatcaaa
     3061 gatcacccga tctactacgc gggtccggcg aaaacccctg ccggttatcc atcaggttca
     3121 cttggcccaa ccaccgcagg ccgtatggac tcctacgtgg atctgctgca atcccacggc
     3181 ggcagcatga tcatgctggc gaaaggtaac cgcagtcagc aggttaccga cgcgtgtcat
     3241 aaacacggcg gcttctacct cggtagcatc ggcggtccgg cggcggtact ggcgcagcag
     3301 agcatcaagc atctggagtg cgtcgcttat ccggagctgg gtatggaagc tatctggaaa
     3361 atcgaagtag aagatttccc ggcgtttatc ctggtcgatg acaaaggtaa cgacttcttc
     3421 cagcaaatcg tcaacaaaca gtgcgcgaac tgcactaagt aacctcttcg gcccagcgcc
     3481 tggcagcatg ctgccaggtg atccccctgg gccacctctt ttgcgattgt aatttcacgc
     3541 ttgctggtga atagtcagta ttttccctga cttgcgaact caccatgacc cgtacactca
     3601 agccgttaat tcttaacacc ggcgcactgg cgctaacgtt aatcctgatt tataccggca
     3661 tttcggccca tgacaaactc acctggctga tggaagtgac accggtgatt attgtcgtgc
     3721 cgctactgct tgccaccgct aaacgttatc cgttaacgcc gctgctctat acgctcattt
     3781 tctttcacgc catcatcctg atggtcggcg ggcaatacac ctacgcgaaa gtccccatcg
     3841 gttttgaggt gcaggaatgg ctggggttga gccgtaatcc gtatgacaag ctggggcatt
     3901 ttttccaggg gctggtgcct gcactggtgg cacgagaaat tctcgtacgc gggatgtacg
     3961 tgcgcggacg taaaatggtg gcgttcctgg tctgctgcgt ggcgctggcg ataagcgcga
     4021 tgtatgaatt aatcgagtgg tgggcggcgc tggcaatggg ccagggagcg gatgattttc
     4081 tcggcaccca gggcgaccag tgggacacgc aatccgatat gttctgcgcg ctgcttggcg
     4141 cattaacgac ggtgatattc ctcgctcgct ttcattgccg ccagctacgg cgctttggct
     4201 tgataaccgg ataacaacgt gcgctttgtt gatgccggat gcggcgtgaa ttccttatcc
     4261 ggtctacaaa atttgcaaat ccaaagcatt acattaagac gtggcctgat aaacatatcg
     4321 catcaggcca ttttcgttag ctattgtcca cgtcaggctt tcacatcact cactgcaccg
     4381 accggaatat cagcatgaac aggctcgggc ggtactttgc gatatttatc cagcagatga
     4441 atctggatcc tgcgcagcgt gtcgccattg aggcgataga aacggaagta gagaatcagc
     4501 gttaccatga aaaacagagt tggtagagca atcataataa actgcatacc taatagggct
     4561 tgcgtagact gttcaacatt cggtacatag ccaatcatcc ctaacacaac cgcaatgaaa
     4621 aaagccgcaa aggctgaacc gcccttcacc accatagtct gcacggagta agcgatactt
     4681 tcacagcgta cgtgcagttt atattcaccg taatcaacgg tatctgccac catgatgacc
     4741 tgtaataccc agaaaagcgc cgttcccaca ttcagcaaaa tacccgcaat cacaatgagg
     4801 acgacgttgt gatagctcat taatgccatt aacaggagaa caccacagct taacaccgga
     4861 agaatagatg ctccggccca taaaatgcgt cgggataatg atttaactaa gcgtgggaag
     4921 aatactaacg tcaccaggtt agcagctccc gcatacgaca gataataggg gaacaaatcc
     4981 gcatcaccga taacatatga gaaataatag atagcaaatc cggtaataat gttgctggct
     5041 acattataag caagagccat acccaagagg catgaaagct gatcgttttt ataaattagc
     5101 gcaacgatgg ctttaagtgt cagatggctt ccttcagcag acggttgatt gtctgacgaa
     5161 aagacttcat gcacattgcg cagagtgatg atggttgaaa caataaaaaa ggcgattagt
     5221 accagagtga acatctgaaa gccaaatccc cgatcgccac cgccgacata attaacaaat
     5281 ggtagcgtca cacctgccgt aacaaagcct gccagactgg caaaaaaacg cggataagga
     5341 accagttgtt cgcgctcacg tttatcgagc gtgatggttg gaaccagcga ccagaaggga
     5401 atatccataa tggtgtaagt catgccccag aggatgtagg tcacgcaaac aaagacaatc
     5461 tgagtagtac cttcaaacag atgcgcacta aagaggagaa ataagattac agagtttgcc
     5521 aacgtaccga tcaggatcca gggtttgaac ttaccccatc gcgatcgcgt agcatttaca
     5581 atccatccca taatcggatc gttaatagca tcccagatcc tcgccaccag aaacaaagta
     5641 ccgaccaaac ccacagacag cccaacgaca tcggtgtagt aatacatgag gtacatatac
     5701 acaatgccga tcgcaaaatc cttcccgaac gctccaaatc cataactgag ttttgtagtc
     5761 attgaaatgc tcatagggta tcgggtcgct gttaccaacg cccgccttct gttattggta
     5821 tcgtcagagg tgtgcgccgg aataaatccc ccggcgcagt agcgtttagt cgcgttttaa
     5881 cggtgcaacc agcctggcag ccagtcgccg tgggcggcaa tcaggtcgtc aacaagagca
     5941 tatatttcat caatgcccag cacggcagca gtatgcgggt ccatcatcgc ggcgtggtaa
     6001 acacggtcgc gattttccgt aagaatagct tcggtcagca gcgtctgtac gttgatgttg
     6061 gtttgcatca gggcggccag atgcgaaggt agcgtaccga ctttggtcgg ctgaatgcca
     6121 ttagcatcaa ccagacaggc tacttccacg caacatcctt gtggcaggtt atcaatcaaa
     6181 ccatcgttac ggacgttgcc gtaaatcaca ctcggctcgc cagtccagat agcgttcatg
     6241 attgtgctgg catattcccg tgacggttta atatcaatcc gggaggcgtt tttatactcc
     6301 tccagctctt tatgccagtt cgccagctgc tcgacgcagc gtttcgggta ctcatccagc
     6361 ggtactttat aacgctcaat caaatcctca cgacctggct taataaacca cggtgtgtac
     6421 tcagcaaaat gttctgacga ttccgtgacg aagtagccca gctttttgaa catttcgtag
     6481 cgcacaatat tctggcagcg agtattgcca tgaatattcg gcttcggtgc ctgccctgct
     6541 tcataagccg ccagcagttc cgggtagaga ttcacataac tgccgtcggc ggttttgcgc
     6601 tccagctcca ggtaaaacgc catatggttg atacctgcgc aacggtaacg cagcgtagct
     6661 gggtcgatat tgaggtcacg cgccagctct tccgccgttc cctgcaccga atggcacagc
     6721 ccgacctgtt tgatatgcgg atagcgggca tacatcgccc aggtattcat cgccattggg
     6781 ttaacatagt tgagcatggt ggcatcgggg cagacttccg tcatgtcctc gcaaatttgc
     6841 cacagatgcg gaatggtacg tagcgcgcgc ataataccgc ccggccccaa cgtatcggca
     6901 atggtttgtt ccagaccatg ccgcttacag acctcgaaat cagtcaccgt gcaaggttca
     6961 taaccgccaa tctgaaatgc caccacgaca aaatcggcat catgtaaggc ttctttctgt
     7021 tgggtgtggc aggtgatttt gccgctggcc cctgctgaat ccataagctt acgcaccaca
     7081 atatgcgact cttccaggcg ggtgggatca atgtccatca gggcaatatg cgccgttttc
     7141 agcgcctcgc gatggaacac atcaccaaga atatttttaa cgaaaatcgt cgaaccagcg
     7201 ccgataaatg taattttggg tgcagtcatc atgcagatct cctggcttgc ttgaataact
     7261 tcatcatggc aggcgaatct gcagaaaacc tccggcttcc aggttgagca ttcccataaa
     7321 ctcagatcct gcgtgaagca gcagtaaatc tgagtttatg gtaataagtt acctgaaaag
     7381 cagagttttc ggcctcatga tgctcggacg ctcccctcgc ctctctggga agagggtgaa
     7441 aaccgtgctc ccactcgcag tcatcctccc tcactcctgc cataattctg atattccagg
     7501 aaagagagcc atccatgaat acagatacgt ttatgtgcag cagcgacgaa aaacagaccc
     7561 gcagcccgct gtcgctgtat tcggaatacc agcggatgga gattgagttt cgcgcaccgc
     7621 atatcatgcc caccagccac tggcatggtc aggtcgaagt gaatgtgcct ttcgatggcg
     7681 atgtggaata cctgatcaac aatgaaaaag tgaatatcaa tcagggtcat atcacgctgt
     7741 tctgggcctg tacaccgcac caactaacag ataccggaac ctgtaagagc atggcgattt
     7801 ttaatctgcc gatgcatctg tttctctcct ggccgctgga taaagacctg attaaccacg
     7861 tcactcacgg catggtgatc aaatcactgg ccacacagca acttagcccg tttgaagtgc
     7921 gccgctggca gcaggaatta aacagtccga acgagcaaat tcgccagctc gccattgatg
     7981 aaattggcct gatgctcaag cgatttagcc tctctggctg ggaaccgatt ctggtcaata
     8041 aaacctcgcg cacacacaaa aacagcgtct cgcgccatgc gcaattttat gttagccaga
     8101 tgctgggctt tattgccgaa aactatgatc aggcgctgac catcaacgat gtggctgagc
     8161 acgtcaaact taacgccaac tatgcaatgg ggatattcca gcgggccatg caattgacga
     8221 tgaaacagta cattaccgcg atgcgcatca accacgttcg cgcgttactg agcgataccg
     8281 ataaaagtat tctcgatatt gccctgacgg caggctttcg ttcaagtagt cgtttttaca
     8341 gcacgttcgg caaatatgtc ggcatgtcgc cgcaacaata ccgcaaactt agccaacagc
     8401 gccgccagac gtttcccggc taaagacgat atcagtatca gccaaaaaaa tagtttagcc
     8461 gtcgataata agcagtgaaa ataatttaaa ataatcacat aacgtattgt ttataaaact
     8521 aaatatttca aaaatgtttg tttttcacgc gctttacagc ccgaaaaggc cggaagatac
     8581 ttgcccgcaa cgaagattcc ttcataaccg ggtaagcaat gatgaaagta ttaattgttg
     8641 aaagcgagtt tctccatcaa gacacctggg tcggtaacgc cgttgagcgt ctggcagatg
     8701 ctttaagcca gcaaaatgtt accgtgatta aatccacctc ctttgatgat ggttttgcca
     8761 ttctctcttc aaacgaagcc attgactgcc tgatgttcag ctatcaaatg gaacatccgg
     8821 acgaacatca aaacgtcaga caattgatcg gtaagcttca tgagcgccaa caaaacgtgc
     8881 cggtcttcct gttgggcgat cgggaaaaag ccctcgccgc aatggaccgg gatctgctgg
     8941 agcttgtcga tgaattcgcc tggattctgg aagataccgc cgactttatc gccggacgcg
     9001 ccgttgccgc gatgacccgc taccgccagc agctgttgcc gccactgttc agcgcgctga
     9061 tgaaatatag tgacatccat gaatattcct gggcagcgcc aggccaccag ggcggcgttg
     9121 gttttaccaa aacacccgcc ggacgtttct accatgacta ctatggtgaa aatctgttcc
     9181 gcaccgacat gggcatcgaa cgaacttccc tcggttcttt gcttgaccat actggcgcat
     9241 ttggcgaaag cgaaaaatat gccgcacgcg tatttggtgc cgatcgctcc tggtcggtag
     9301 tcgtcggtac ttccggctct aaccgcacca tcatgcaggc ttgcatgacc gataacgatg
     9361 tcgtggtcgt tgaccgtaat tgccataaat ccatcgaaca aggcttgatg ctgacaggcg
     9421 cgaaaccggt ctatatggtg ccaagccgca accgctacgg cattatcggg ccaatctatc
     9481 cgcaggaaat gcaacctgaa accttgcaga agaaaatcag tgaaagcccg ctgaccaaag
     9541 acaaagccgg gcaaaaaccg tcttactgcg tggtgaccaa ctgcacctat gacggcgtgt
     9601 gttataacgc taaagaagcg caggatctgc tggaaaaaac ctccgatcgt ctgcactttg
     9661 acgaagcctg gtacggctat gcacgtttca acccgatcta tgccgatcac tatgccatgc
     9721 gcggcgaacc tggcgatcac aacggtccta ccgttttcgc cacccactcc acccacaaac
     9781 tgctgaatgc gctgtcacag gcttcttata ttcatgtacg tgaaggtcgt ggggcgatta
     9841 acttctcccg cttcaaccag gcctacatga tgcatgccac cacctccccg ctgtatgcca
     9901 tctgcgcatc caacgacgtg gcggtgtcga tgatggacgg caacagcggc ctgtcactga
     9961 cacaggaagt gattgacgaa gcggttgatt tccgtcaggc gatggcgcgg ctatataaag
    10021 agttcaccgc tgacggtagc tggttcttca aaccgtggaa caaagaagtc gtcaccgacc
    10081 cacaaaccgg caaaacctat gactttgctg acgcaccaac caaactgctg accaccgttc
    10141 aggactgctg ggtaatgcat ccgggcgaaa gctggcacgg cttcaaagat attccggata
    10201 actggagtat gctcgaccca attaaagtca gcatccttgc tccgggaatg ggtgaagatg
    10261 gtgaactgga agaaaccggt gttccggcgg cgctggtcac tgcctggctt ggtcgccacg
    10321 gcattgtgcc tacccgcacc actgacttcc aaattatgtt cctgttctct atgggcgtaa
    10381 cccgtgggaa atgggggact ctggttaaca ccctttgttc cttcaaacgc cactatgacg
    10441 ccaacacacc gctggcgcag gtgatgccgg aacttgttga acaatatcct gacacttacg
    10501 cgaacatggg gattcacgat ctgggtgaca ccatgtttgc ctggctgaaa gaaaacaacc
    10561 ctggcgcacg gttgaacgaa gcctattccg gcctgccgat ggcggaaatc accccgcgtg
    10621 aagcgtacaa cgcgattgtc gacaacaatg tcgaactggt atccattgaa aatctgccag
    10681 gacgcatcgc ggcaaactca gttatcccgt atccgccagg aatcccgatg ctgctgtctg
    10741 gtgaaaactt cggcgataaa aacagtccgc aagtaagtta tttacgctcg ctgcaatcct
    10801 gggaccacca tttccctgga tttgaacacg aaactgaagg gactgaaatt attgacggta
    10861 tttaccacgt tatgtgcgtg aaagcgtaac cactattccg ctgaaggcgt aattgtttaa
    10921 ataacattac gccgcctggc cttaggcctt ttgagtatgg caacgttttc ataaaaattg
    10981 ctgcaaacaa aaatgtcata ctttttgcgc ggccccaccc cg
//
LOCUS       DAZFRJ010000148        10980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000148 DAZFRJ010000000
VERSION     DAZFRJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.150"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..413
                     /locus_tag="ACHKUN_003525"
     CDS             <1..413
                     /locus_tag="ACHKUN_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661722.1"
                     /translation="ISNDNEIESVQNAVDNGTDSSRSHFERALQLMTDREQPDYRNSI
                     KESISAIESLCRKITGNDKGTLGACLKAIEEKGYIHSAMKGAFSQLYGYTSDQGGIRH
                     ALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
     gene            complement(462..1034)
                     /locus_tag="ACHKUN_003526"
     CDS             complement(462..1034)
                     /locus_tag="ACHKUN_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661723.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            1135..2305
                     /locus_tag="ACHKUN_003527"
                     /pseudo
     CDS             1135..2305
                     /locus_tag="ACHKUN_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2388..2645
                     /locus_tag="ACHKUN_003528"
     CDS             2388..2645
                     /locus_tag="ACHKUN_003528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661724.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(3203..3970)
                     /gene="fecE"
                     /locus_tag="ACHKUN_003529"
     CDS             complement(3203..3970)
                     /gene="fecE"
                     /locus_tag="ACHKUN_003529"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO6661725.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(3971..4927)
                     /gene="fecD"
                     /locus_tag="ACHKUN_003530"
     CDS             complement(3971..4927)
                     /gene="fecD"
                     /locus_tag="ACHKUN_003530"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO6661726.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(4924..5922)
                     /gene="fecC"
                     /locus_tag="ACHKUN_003531"
     CDS             complement(4924..5922)
                     /gene="fecC"
                     /locus_tag="ACHKUN_003531"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO6661727.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(5919..6821)
                     /gene="fecB"
                     /locus_tag="ACHKUN_003532"
     CDS             complement(5919..6821)
                     /gene="fecB"
                     /locus_tag="ACHKUN_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO6661728.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(6866..9190)
                     /gene="fecA"
                     /locus_tag="ACHKUN_003533"
     CDS             complement(6866..9190)
                     /gene="fecA"
                     /locus_tag="ACHKUN_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO6661729.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9277..10230)
                     /gene="fecR"
                     /locus_tag="ACHKUN_003534"
     CDS             complement(9277..10230)
                     /gene="fecR"
                     /locus_tag="ACHKUN_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO6661730.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10227..10748)
                     /gene="fecI"
                     /locus_tag="ACHKUN_003535"
     CDS             complement(10227..10748)
                     /gene="fecI"
                     /locus_tag="ACHKUN_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO6661731.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            10885..10953
                     /gene="ykiE"
                     /locus_tag="ACHKUN_003536"
     CDS             10885..10953
                     /gene="ykiE"
                     /locus_tag="ACHKUN_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO6661732.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
BASE COUNT         2536 a         2926 c         3016 g         2502 t
ORIGIN      
        1 ctatttcaaa tgataacgaa attgaaagtg ttcagaacgc agttgataat ggcactgatt
       61 catcgcgtag ccatttcgaa agggcgttac aactaatgac ggatagagag cagccagact
      121 acagaaactc gattaaagag tctatctctg caattgaatc tttatgcagg aaaatcacgg
      181 gcaatgataa aggaaccttg ggggcttgtc ttaaagctat agaggaaaaa ggatatatac
      241 actctgcaat gaaaggcgct tttagtcagc tctatggtta tacaagtgat cagggtggaa
      301 tacgtcatgc gctaacagaa gaagatgtaa atcctacctt agctgaagct aaatttatgt
      361 tagtcacttg cagtgcgttc agtaattatc tgttgtcaaa aataagtgat taattacagg
      421 tttatagtta cgagtgggtt agttacccac tccctttaaa attattcacc agactctaat
      481 cttttttctc ttttctcatc tctccttttt attgccaaat cgtgttgctc ttgcagatac
      541 gtgcgttctt cgtttctatc gcttgaagta tatacgtctg cttttacttt tttagctcct
      601 tgtgcaatag gtttccaatc ctctcttata cctttaattt gttctgcaat ttgcgtcaat
      661 gggcttctcg ctcctagata acttattcct tcaaatgtcg ccatgtcata cacatattca
      721 tttgtttctc tatcgtttga tttaggtttg cttgcccagc ttatattaat attataaatt
      781 tgatctttga tttgttcata cttacagaga ttgctggtaa gtgattgccc attttttaac
      841 acgcttacct tactaaacgg aatctcactt ttttctctat gctccgcatt tactagtgga
      901 ggatcaaaca caacctcaac attataggca ggtgcgttcc ctgtattagc aatgttaata
      961 tcaaagtaaa aagcggccca agggtttggc tcaagtgtta caactatatg gggttgagct
     1021 gatgcgtcaa ccattctttt tgtctcgtct gctctgaagt ggtgaagtgg tttactgaat
     1081 ttggccacct gaacagaggt gatatgctca cctcagaaca acacaggtgc tccaatgaaa
     1141 aaaagaaatt ttagcgcaga gtttaaacgc gaatccgctc aactggttgt tgaccagaca
     1201 tacacggtgg cagatgccgc caaagctatg gatgttggcc tttccacaat gacaagatgg
     1261 gtcaaacaac tgcgtgatga gcgtcagggc aaaacaccaa aagcctctcc gataacacca
     1321 gaacaaatcg aaatacgtaa gctgaggaaa aagctacaac gcattgaaat ggagaatgaa
     1381 atattaaaaa aggctacaac cgcgctcttg atgtcagact ccctgaacag ttctcgataa
     1441 tcaggaaact cagagcgcat tatcctgtgg tcacactctg ccatgtgttc ggggttcatc
     1501 gcagcagcta cagatactgg aaaaaccgtc ctgaaaaacc agacggcaga cgggctgtat
     1561 tacgcagcca ggtacttgaa ctgcatggca tcagccacgg ctctgccgga gcaagaagca
     1621 tcgccacaat ggcaacccgg agaggctacc agatggggca ctggcttgct ggcagactca
     1681 tgaaagagct ggggctggtc agttgccagc agccgactca ccggtataag cgtggcggtc
     1741 atgagcacgt tgctatcccg aatcatcttg agcgacagtt cgccgtaacg gaaccaaatc
     1801 aggtgtggtg cggtgatgtg acctatatct ggacgggtaa gcgctgggcg tacctcgccg
     1861 ttgttctcga cctgttcgca agaaaaccag tgggctgggc catgtcgttc tcgccggaca
     1921 gcaggcttac catgaaagca ctggaaatgg catgggaaac ccgtggtaag cccgtcgggg
     1981 tgatgttcca ggcgatcaag gcagtcatta tacgagcagg cagttccggc agttactgtg
     2041 gcgataccgg atcaggcaga gtatgagtcg gcgtggaaac tgctgggata acagcccaat
     2101 ggagcgcttc ttcaggagtc tgaagaacga atgggtgcca gcgacgggct atgtaagctt
     2161 cagcgatgca gctcacgcaa taacggacta tatcgttgga tattacagcg cactaagacc
     2221 gcacgaatat aatggtgggt taccaccaaa cgaatcagaa aaccgatact ggaaaaactc
     2281 taacgcggag gccagtttta gttgaccaca acagactacc tgaagggagc cgcggtcgcc
     2341 tagcagttgc agtagcagga gatcatccag ccgcagtaca ggtcacgatg actctggtta
     2401 atgataccgg ctttgacccc gtattttccg gctctatcgc tgaatcatgg cgtcagcagc
     2461 cgtgcacacc atcctattgt tgtgactggg aggctgccac catgcttcgc gctttccctc
     2521 tggcgaaaaa gggagaagga cgggcccgtc tgccttcact ttatgccagc ttcggtaagc
     2581 tgggtgagac accgactcat aaagatatca ttgataacaa tcgatccatc aactggcctg
     2641 tataacgtgg ctgccggtga ttaagaaagc tgcacctacc taagtagtag caaacgcaca
     2701 ctttttagaa aaatcgatgg tcagaaactg gattagcaat tccgttccag ggttgctttt
     2761 gatttacgtt ggcgtctgat cattgattta tcctcaaaag cccaacctca ttggtaatga
     2821 accagctccg tgaatgtccg ctctggcaca gagcgaaatt ttttgatctc cccccctgaa
     2881 atctaaactt agtcatgtca cgtttttggg tttctaaaat tttaacttcg cgtttttcgt
     2941 tgccgtaagg gttatacaga aatgtccgtt aagcagagtt caaaattgat tgccgtgatc
     3001 acgactggtt tgaaagccgc gcccaagcct gtacagctct ggtttgcgtt gattatgaac
     3061 ctgtcagcct aaagcaagcg gatggacgat gagtattggt aatctttcag agtccggaaa
     3121 agttcagccc cagtctgaac aggcttgctg gcgccagtcc agtttcattc agtcgtggtt
     3181 tggttcttac ggcctgtgca atctacctca ttaggcacat cggcctgcca gataccggct
     3241 cggggtgtat ttccgcttcc acgctgaata ctgttctcag caatcctggg gtcatcacct
     3301 cttctggtgt gccttgcgcc ataacatgtc cgtttgccat taccaccagt tgatcgcagt
     3361 accggctagc ctgattaagg tcgtgcagca cagcgaccac cgttttcccc tgagtccgga
     3421 gttcgcccat caaccgcatc aggtccacct ggtgattgat atcaagatag gtggttggct
     3481 catcaagtaa tacaacgggc gtattctggg ccaggaccat cgccagaaat gcgcgctggc
     3541 gctgaccgcc ggaaagctcg gttaaccgac gaacggcaag atgattgatc cgggtctggt
     3601 tcatggcgac attaactcgt gcattgtctt cagcggagag acgcccccag agtgacagcc
     3661 agggattacg accatacgaa accagctcct ggactgtgat cccctctggc gttaaatggt
     3721 gctgaggcag cagcgaaagc ctgcgggcca actggcgcga tgagagcata tttatgggat
     3781 tatcgccgag aaatacggtg ccagactgcg gcattaaaag ccgcgaaaaa cagtttaaca
     3841 gcgtcgattt cccgcaaccg ttaggaccga tcagggcggt gatcttcccc gttggcagtg
     3901 agagtgaaac gtcgttaagt accttgtctg tcccgtaact gaccgtcaga ttttcagttc
     3961 gtaaagtcat ttatcgcatt ctcacaagca accagacaaa ccacggcgca ccgataatgg
     4021 cggtcagcac gccaaccggg agctccagtg ggggatgaat aattctcgcc agcagatcgg
     4081 caaccaccaa cagcaacgca cctgtcaggg ctgaaacagg cagcagtctg cggtgacgtc
     4141 caccggtgat gctacgcatc atatgcggca ccacgagacc aataaagcta atcgggccgc
     4201 aggcggccac gccggtagat gtcatggcga cagctagtaa caaagcccag aatcgggtat
     4261 ggggcaccga cacaccgagc gtggtggcgc gcgcatcgcc gagtgcaagg aggtcgagat
     4321 cgcggcaaaa actcaggctc agcggcagaa ataaaatcat cagcgggatg gcaatcttca
     4381 caaagctcca gtcacggccc cataagctgc cggtcagcca cagcagggcg ttgttcacat
     4441 cctgtgggcg cgagagcatc agataatccg tcaggctggc ccagcatgca gaaagcgcca
     4501 cgccggtgag cgccagcttc atcggctggt gggtctttgc cagcatcttc agtaatatca
     4561 accccgccat gccgcccgca aaggccagca gcggcagcac catcacgggc agtgacggca
     4621 taagaagtag agcccccaca gaggccaggc tggcggcatg gttaacgccg agaatatccg
     4681 gtgatgccag agggttgcgc acaatcccct gtatcagcac gcccgccacg gcgagggctg
     4741 caccgacaaa cagtgccagc agcaagcgcg gcagtcggta ctccatcaat acataataat
     4801 gctcgcgtcc ggcctgccag tcggtcagca gcgcgcgcca cggcacgggg atcactccca
     4861 tatggagtga taacagcgca cagcccgcca gggcaagggt gatgaaaata accagcgcaa
     4921 ttttcatcct cgcctcctca caagccagac aaagcaaggg ctgccaatca gcgccagcac
     4981 tgcgcctgcg ggcagatctc cggggaaggc cagcgcgcgt gcgagtacat ctgccagcag
     5041 catcagcgtg gcccccagca gcatgctcac tggcagtacg ttgcgctgat cgaagcctgc
     5101 ccagaagcgc gccagatgtg gcaccagcag accgataaac gccaccggac ctgccacgct
     5161 gacgcacgca ccaaccagaa gcagcactaa catattgatg accaaacgta gcctcgtcag
     5221 gttcactccc agcgtatggg cggtgctgtc gctgaggttg agcaggttca gttgattcgc
     5281 cagcagcaac acgacaggga ctgcagtgac caccaccggc aagagctgcc agacatcctg
     5341 ccagcgggcg tgggacactc cgcctgccag ccagtaaaag atgccgtaag catgatcttc
     5401 ggccagcagc agggtgatgc gggtaaggcc catacaaaag gccgacagcg cgatactcgc
     5461 gaggatcagt ttgtttctgt catgggtatg acgaaatccg cctcctgcgg tcatgaccag
     5521 cagccagctc acgccgcccc cgcatgccgc gatgaacgac agagaatagc ctgcaatcgg
     5581 cgtcggactc agcgcgctgg taagcgccat agccagcgcc gcgccgctgt taatgccgag
     5641 cagtgaagga gaggccattg ggttgtgggt cagggtttgc agcagcgtgc ccgcgagcgc
     5701 caggcttgcg ccgatcagaa cggcgaccag gcttcgtggc aaacgaaggt tttgcaccag
     5761 cgcttctggt agcgttggcg tgtgtccagg cagcagggcg cgggttgcat ctgctccgga
     5821 aacaggaatg gccgagtagc aaaacagact cagccagaaa ataataataa gtgctgcaac
     5881 gggaagcccc cacagcagca ccgggtgttt tatcgcggtc atttcacaac ggtaagcggc
     5941 tgatggtgga agatttttac cgtgtcagcg gcaatacgct ctgcagcaaa aataccgcgc
     6001 atccgcgccc aggtgttact gtcgaccgaa gcaacctgct gcttctgcgc ggcggttaac
     6061 atctgccaga gcggatcttg ttgccagcgt ttaacaatgc tctcttcgcg atagtgggca
     6121 accagcagcc aggcaggatt gaccgccagc agttgctcca ggccgatgga cggcatggac
     6181 gcacccgcca tcgcagcggg aacgttcagc cccagagagg ccagcacgct gccggtccag
     6241 gtctcctgag tatgcaggtt gaattgctgt tcgcgtgatg tgccaaaggc cacgcgtgtc
     6301 cctttgggaa gctggctggc ccactgcgcc atcctctctt tatgttgttc caggcgtgcc
     6361 tgcatctctc gctttttacc caccatttcg ccgatgatag ccgcagattg caaattttca
     6421 gcgtaggttt cgttgcggga cttaagcagc agtaccggcg cgatttgctg caaggcgatg
     6481 taaacccccg catggcgact gctgtcggca atgatcaggt ctggtttcag agcggcaatg
     6541 gcttccaggc tcggctgcgc gcgcgttccg acggactgcc acggtttcag gtgcgcacgc
     6601 acttcgggca ggatgcgttt tgcatcgtta tcgtcggcaa taccgatcgg gctgacgtcc
     6661 acggcggcca gcgcatcggc gaacgagagt tccagcacca caatccgttg tggcgttttt
     6721 tcgagtgtaa acgtgccgtg ttcgtcctga accgtggcgg caaaggcgtg gctgatcacc
     6781 agcagcaggc ctgcaaaaac aaaacggata aatgccaaca taatcacatt ccagctaaaa
     6841 gcccggcaag ccgggcgtta acacatcaga acttcaacga cccctgcata tacagcgtgc
     6901 gcggctggcc tgcatagatg cctttgttgt tgtcgtcata agagcggatg aagtagtcct
     6961 ggtcgaagat gtttttcaca ccgaatgcca ggttcagatc tgccatctgc gggccaaaat
     7021 catacgccac gcgtgcgccc cagagcatga agccgggaat gcggccggta ctgccgtcgg
     7081 cgctctcttt caccgtattg gcgttatccg caaactggct ggactggaaa tcgctgttca
     7141 gattgaacgt ccagtttcct ggcttgtagt ccacgcccag cgtgccttta tgtttcgggg
     7201 agaatggtac cagattgccg taggtgtcgc ctttctcgcg gatttccgcg ttcacatacg
     7261 catagctggc gtagatggaa acgttatcaa gcgttggcgt tagcgtaccc agatcgtaac
     7321 gtgcctgcgt ttccagcccg gtatggcgcg ttttgccacg tgcagtgacg gtgtcgttgg
     7381 tctggttgga gtcgtactga ttgttaaagt taatcaggaa cagccccatt tccgccgtca
     7441 gcgcgccgtc gtcgtagcgg gtaccgagtt cccaggttcg cgctttttcc ggttccacat
     7501 tgccgctttg cacagccttg ccaatctggc tgtactgtac ggtgccgaac gagccttcag
     7561 tgtttgcata aagattccag ctgtcagtca ggtgatagag cacgttcaac gccggaagcg
     7621 gtgcgttata gctcacttct tcgtgcgtgc ctgtgatggc gttgttctgg tatgactcga
     7681 tatgttcgaa acgcataccc ggcgtgatgg tccagttgcc gatgtcgatt ttgtcatcca
     7741 gataccaggc gtgcgcctcg gtgccggaac gcgtatcgcg gtcgtaaggg cttgagccgg
     7801 acggcaactg cccgctgctg gtggcggtgt agtaacgcat ttcatgcgtt gattcattca
     7861 aatagcgata gcccacgccc acttcgtgcg cggaagggcc gatcataaag atctggctgt
     7921 agcgtggctc aataccgcgc acccagtagt tacgcggcga gagggtgatg cgtttgcctt
     7981 gctccaggta gccgctgcgc agggtttggg tgtagaaccc ctgaatgttg aatttatgct
     8041 ggctgtctgg ctggaactgg tagcccaggc tcgccagctt gcgacgaccc cagaagcggt
     8101 catacgggcg ggtggattgc cagcgatcgg cgtcgtaatc cgcgcgagac aggccaccgg
     8161 gcatgtcggc ttcaccatcg taatattgca gcaggctgtt gaaggtgtgc acctcatccg
     8221 gcgcatattt gcttttcagc atcaggtcgt cgatgcgggt ggcgctgtgc tcgcgccagt
     8281 cactgccgcg cgtgccggag tagagcagcg cggtgccaaa accgttgtcc gctgtgccgc
     8341 ccaccatcag gttgtgcgtc tctttcgggt tgttttgtga agaggttggg ctgagctgac
     8401 cttccacgcc cgcctcgata ccaaagtcct gcggaatggc acgggtaaca aagttcacca
     8461 cgccgcccac gctctgcggt ccgtaacgca ccgcaccacc accgcgtacc acgtcaatgg
     8521 catccatgtt gccgagcgaa acgggagcca gtgaaagctg cggctgaccg taaggggcga
     8581 aggggacggg gatgccgtcc atcaggacgg tcgagcggct ggcgaggcgc gggctcaggc
     8641 cccggatgcc aaagttcatc gccaggtcgt ggctgccggt gccgttgttt tccggcgcgc
     8701 tgacgccagg gatgcggtta agtacctcac gcatggtggt tgcgccggtt ttggcgaaat
     8761 cctcacggcg gatcacgtca cgcgcgccag catgttcaaa tacgtcgttt tcacgcgcat
     8821 cacccagcca gtcgccgacc acggtcaggg catcttcttt tggtgcgggc gcgggctcca
     8881 gcgtccagct gttatttccc agcggtttta cctgcagtcc gctgccgtcc agcagttgtt
     8941 gcaggccgct ctcgacgtcg taatcgccgt gcaggccgtt gctctgcttg ccgcgcgtca
     9001 ggctggcgtc aaccgagagg gtaaatccgc tgtgtgcggc atactgattg agcgctttat
     9061 cgagcgatcc cggtgcgata ttaacctgtg cagcaaaagc ggaaaacgag agaccggcca
     9121 gcggcagcag gctcaggcga atggcgttaa ccaaaggtgt tgttttacga aaaacgcgta
     9181 acggcgtcat accttcccca tcatcatttt tgttgtgttc agctatgagt cgaacgagaa
     9241 gggtaaaaag gacaatcgaa ataagaatta ttttccttac agtggtgaaa tgtttatcca
     9301 gtaccgcgta atagactgaa ttttaacggg aagcgtttgc gcgataacgt tcaggatcgc
     9361 atcggtattt ttcagcggga acgtcccgct caggcgcagc ccggcaacgg cgggatcgca
     9421 gcgcagcacg ccgttgcggt aacgggttag cgtggctatc acctcaccca gcggtttatc
     9481 gctgaagctc aggatgtcct tcgtccagct tgtactctcg tcatccagcg gtttcactgc
     9541 gccaaactca gaggcgctga actgcaggct ttcaccagcg ttcacgatgc gtttttgcgc
     9601 gggggcactg gcgagaagca cttccacagc gtgctgctgc acgtcaagct gcgtgaaatt
     9661 atcctgctgg cggacggtaa attctgtccc taaagcagtg agctggccct gacgggtcag
     9721 gacacggaag gggcgttgca gggcatcttt cgcggtagta atggcgattt caccgtacca
     9781 gagccggacg gtgcgctgat gcgcatcaaa acgcacatcc gccgcgcttt gggtattcag
     9841 cgtgagcagg gagccatctt ccagttgctg acggctgact gtgcctttgg cggtgcggta
     9901 atctgcccgc agaccttcgc cggtttccga ctgccagagc tgccagcctc cgccagcgcc
     9961 gagcaacagc agtaatcctt tcatcacgtg acggcgggtg aggcgggtat cgtgcaacgc
    10021 cctgctggct acgtcgccag gcacaccgcc aagctggttg cgcaggtttt caacctgctg
    10081 ccaggcccac tggttatcct gatcctgttc ataccactgt tgccagcgcg cttcctgttg
    10141 tgggctgacg cgctcgccgc ttagcacggc ataccagtgg gaagctgaac gcagcgcctg
    10201 acggcgggaa tcggttaaca aaggattcat aacccatact ccagacggaa cagcaggcag
    10261 tgctcgacgg ctttcgccac gtattttttc acggagctga tggaaacacc gagtttgtgc
    10321 gcaatctcgc tgtatgtcag accatccagt tgcgaaagca gaaacgcttc acgtgttttg
    10381 ccgtttagcc cgtccagcat gctgtcgagg agttgtaggg tctcgagttg gctttcgcgt
    10441 tcctcaggtg aaggcgctcc cccctccggc ataagcgcca gcatctccag atacgctttt
    10501 tccagcgcgt ttcggcgaaa caggtccacc atcacgcgtt tggcgatagt gcagaggaag
    10561 gagcgaggat cgcggatcgt cgagagcgtt tcgctgacca ttacccgcaa aaaagtgtcc
    10621 tgggcaatgt catctgcatc aaaagcagac tggagtttgc gcgtcagcca gcttttcaac
    10681 cagccgtgat gtgtgccata aagcgactcg aacgttaagg aagctgtggt agtggcgcgg
    10741 tcagacatgc ggagtgcatc aaaagttaat tatcacgtag tcatattaat atgagaatgg
    10801 ttatcattac aattggaaat aaaattgttt ccaatagaca tttttaacat gttgtttttc
    10861 taagtgttat aaggtaggta taaaatggga tggagcctct gcttctggca tgtgtcggtc
    10921 agaatgactc atgatgtggt ctgctattat tgacatcctc actgccctaa aggatgggga
//
LOCUS       DAZFRJ010000149        10900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000149 DAZFRJ010000000
VERSION     DAZFRJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.151"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..859
                     /gene="adiY"
                     /locus_tag="ACHKUN_003537"
     CDS             98..859
                     /gene="adiY"
                     /locus_tag="ACHKUN_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO6661733.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            996..2333
                     /gene="adiC"
                     /locus_tag="ACHKUN_003538"
     CDS             996..2333
                     /gene="adiC"
                     /locus_tag="ACHKUN_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO6661734.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2437..4080
                     /gene="eptA"
                     /locus_tag="ACHKUN_003539"
     CDS             2437..4080
                     /gene="eptA"
                     /locus_tag="ACHKUN_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO6661735.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4077..4745
                     /gene="basR"
                     /locus_tag="ACHKUN_003540"
     CDS             4077..4745
                     /gene="basR"
                     /locus_tag="ACHKUN_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO6661736.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4746..5846
                     /gene="pmrB"
                     /locus_tag="ACHKUN_003541"
     CDS             4746..5846
                     /gene="pmrB"
                     /locus_tag="ACHKUN_003541"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO6661737.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5822..5911)
                     /gene="pmrR"
                     /locus_tag="ACHKUN_003542"
     CDS             complement(5822..5911)
                     /gene="pmrR"
                     /locus_tag="ACHKUN_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO6661738.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6023..7525)
                     /gene="proP"
                     /locus_tag="ACHKUN_003543"
     CDS             complement(6023..7525)
                     /gene="proP"
                     /locus_tag="ACHKUN_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO6661739.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7789..8667)
                     /gene="rdcB"
                     /locus_tag="ACHKUN_003544"
     CDS             complement(7789..8667)
                     /gene="rdcB"
                     /locus_tag="ACHKUN_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO6661740.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQEGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8664..10892)
                     /gene="crfC"
                     /locus_tag="ACHKUN_003545"
     CDS             complement(8664..10892)
                     /gene="crfC"
                     /locus_tag="ACHKUN_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO6661741.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2723 a         2845 c         2694 g         2638 t
ORIGIN      
        1 tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc
       61 gagaactggt cttttatttg cactcaggag tacatgtatg aggatttgca gcgaccaacc
      121 ttgtattgtt ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat
      181 tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc
      241 ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg
      301 gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact
      361 caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc
      421 cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg
      481 ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag
      541 tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt
      601 agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac
      661 cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat
      721 ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt
      781 tatttctgtc tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag
      841 agaacgcact gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag
      901 cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt
      961 attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca
     1021 aagtgggctt aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt
     1081 ttctgttacc tgcaaacctg gcctctactg gcgggattgc catttatgga tggttggtga
     1141 cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgatccaa
     1201 gtcctggtgg ttcttacgcg tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa
     1261 ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg
     1321 taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct
     1381 gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc
     1441 gtgtgcaggc cgttgccacc gtgctggcgc tgatccccat cgtcgggatt gccgtatttg
     1501 gctggttctg gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca
     1561 ccttcggtgc aattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa
     1621 gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca
     1681 ttggtggggt attgattgcc gccgtttgct atgtactttc taccaccgcg attatgggga
     1741 tgatccctaa tgccgcactg cgcgtttctg cttcgccatt cggtgatgcc gcacggatgg
     1801 cgttgggtga caccgccggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt
     1861 cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac
     1921 tgttcccacc gatttttgcc cgtgtaaata aagcgggcac gccagtggcg gggctgatta
     1981 tcgtcggtat tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag
     2041 agttcggtct ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct
     2101 gtgcggcgtt actgctgctc ggtcacggtc actttggtaa agcacgcccg gcatatctgg
     2161 ctgttactac cattgccttc ctctactgca tttgggccgt ggtggggtcc ggggcgaaag
     2221 aggttatgtg gtcatttgtc accctgatgg taatcaccgc catgtatgcg ctgaattaca
     2281 accggctgca taaaaacccg tatcccttag atgtaccaat aagcaaagat taattcccct
     2341 taatccagca aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc
     2401 gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga
     2461 ccctctttga atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg
     2521 aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta
     2581 ctggttttct tgtcgatgcc ggtcgtcgct ttcagcgtga ttaatattgt cctgacacta
     2641 agctctttct tatggcttaa tcgaccactg gcctgcctgt ttattctggt tggcgcggct
     2701 gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt
     2761 attgatacca ctccggcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg
     2821 ggattcagcg gcgtgcttgc tgcgctgatt gcctgctgga taaaaatcaa acctgccacc
     2881 tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt actactgatt
     2941 ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag
     3001 ctggtgaaat ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat
     3061 cagcgactgg caaatctacc gctggtgcga attggtgaag acgcccaccg caacccgtta
     3121 atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg
     3181 gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc gaaagataac
     3241 gtggtctatt tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc
     3301 atgttctcgg atatgccgcg tgagcattac aaagaagagc tggcacagca ccaggaaggc
     3361 gtgctggata tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc
     3421 tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctggt
     3481 cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc
     3541 aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc
     3601 tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag
     3661 atccagacct gtagcaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc
     3721 gactatattg ttgataaagc gattaatctg ctgaaagaac atcaggataa atttaccacc
     3781 agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac
     3841 ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccgatgct gctgtggctg
     3901 tcggaggatt atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa
     3961 acgcaacact attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag
     4021 acgaagtatt accaggctgc ggatgatatt ctgcaaactt gcaggagagt gagtgaatga
     4081 aaattctgat tgttgaagac gatacgctgt tattgcaggg attgattctg gcggcgcaaa
     4141 ctgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg
     4201 ccggtcatta cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt
     4261 ttctcgcccg tatccggcag aaaaaataca ccctgccagt actgatcctc accgctcgcg
     4321 atacgctgac cgacaaaatc gctgggctgg atgtcggtgc cgatgactat ctggtgaagc
     4381 cttttgcgct ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc
     4441 agggcgaaag tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat
     4501 ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa
     4561 tgctcaaagc aggcagtccg gtgcatcggg aaattctcta caacgacatc tataactggg
     4621 acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg
     4681 gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa
     4741 actaattgaa tctgatgcat tttctgcgcc gaccaatatc gctgcgccaa cggctgatat
     4801 tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg
     4861 aaagtaccga gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc
     4921 gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta
     4981 tggtcagcct gacgctattt atctgctatc aggcggtgcg ccgcatcacc cgcccgctgg
     5041 cggagctgca aaaagagctg gaagcgcgca ctgccgacaa cttaacgccc attgccattc
     5101 acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt aaacgatctg gtcagtcgcc
     5161 tgaccagcac gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa
     5221 cgccactggc gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg
     5281 tagcaccgtt agtggcacga cttgatcaga tgatggagag cgtctcccag ctgctgcaac
     5341 tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag
     5401 atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc
     5461 tgctattgcc agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga
     5521 tgttattgcg aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta
     5581 tgattaagct gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta
     5641 ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg
     5701 gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt
     5761 ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag
     5821 atcagaacgt ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg
     5881 tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga
     5941 gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc
     6001 acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg
     6061 tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat
     6121 tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg
     6181 cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca
     6241 ccgccactac catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc
     6301 aggccgccag tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca
     6361 gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgcccg
     6421 taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt
     6481 tactgttaat cagaataaac gccgggatcg ccaacacaaa cagggcaaca ctaccaagta
     6541 gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca
     6601 gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt
     6661 tatgcgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa
     6721 ttaccagacc aatacatgtc aacaggctgc gccagtgttt ggtggcaatc tctttaaacg
     6781 agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcgacatgct
     6841 gctggaacgc cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg
     6901 gcagagcaat aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa
     6961 tggtcgaaat taacaccact acgccagcac ccagcacaaa cccggcaata gaaccgaagt
     7021 ccagccagct gcccataaag ccacgtttac ggtcaggtga gtattccgca acaaatatcg
     7081 acgccccggt atattcaccg ccgaccgaga aaccttgtgc catcttacag atcaacagca
     7141 gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac
     7201 tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca
     7261 acataccaaa gaagagcccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa
     7321 gtgcagcaac catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt
     7381 aagcaacaaa accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg
     7441 cggtaatggc tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaattg
     7501 gttttacttt tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac
     7561 gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct
     7621 gtaacaagcg tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt
     7681 tgacgcctga attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc
     7741 aacttcacac atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt
     7801 tacagagaag atttcctcga gcattgcccc cgccagacgc tgcgctgaac agagtcgtgg
     7861 catccccagc gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc
     7921 tgccgtggtc cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac
     7981 ggtcgaagag gcaaaccact gatggtaata gtgacgcgtg gtcggggccg cattgatact
     8041 ctctgccaga tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg ccaccgcttg
     8101 cgggcttccc agacgacagg catcgccaaa tgccccccaa cgcaactctt cgagaacgat
     8161 aaaacagcgc ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat
     8221 ttgctcgctc tgatcacctt cctgcaccag taggcctcgt tgcgttaacg ccttttcctt
     8281 ccagcggcaa cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg
     8341 gaaagcagag tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg
     8401 cgtcagtgcc agatgaccgg gatccagcat cgacgccagt ttttccacca atgtcagacg
     8461 caggctaagc ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacgcgcgc
     8521 aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg gaaggcgggc
     8581 atcatcgccc tgcgccagat ccactaaaaa gcggggataa accgactcca gcacgcgacc
     8641 ggggccgtca agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca
     8701 tcgcgtaaca gctgggcgtc ggtgtaaatc cagccgtgag tcatcacagt ccgctgcaat
     8761 tgctgctgca tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt
     8821 aaacgcgtct gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct
     8881 gccgtaacag attcatcgac ctgctctgcc agaactttag ccatttgttg gcaataaaga
     8941 tcgacttctg cgcttaaagc tcgttgcaac acactgtaat caaccgtctc tgtcggggat
     9001 ttctcatttc cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca
     9061 acgcctaaac gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac
     9121 agctggcgag tgttgaagtg gggaactgcg ccgtgaaaaa caggaaaatg aaacccagga
     9181 cgaaaccctg actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg
     9241 gttaacaccc ggcaaagggt ggattccagc tcggcaaaac gcagcgtaaa gtcgcgactg
     9301 atggtgttct gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc
     9361 tccgaatcat cctgaaacaa cgcggctaac tgtgcattca ccgcatcttg ctgttgacgc
     9421 agaaagtggt tggcggaggc caaggccagc tcgatttcat gtttaatctc gccgctcacc
     9481 tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc
     9541 aattgttcac aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc
     9601 tgcgcgtaat tcaacagttt atgcgcggca gatcgcagag catacaacga ggcgttagcg
     9661 taagcggcat gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga
     9721 tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg
     9781 agcgcggcat gggcaaaatc ttccacccag cgttgttgct ctggcggtgg taacttaccg
     9841 ttgttggcta actcatggcg cgcccgattc gccaggtagc cccacatcga cgacaccgga
     9901 aatatctgct gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc
     9961 tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc
    10021 ggcaccgatt gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc
    10081 agttgcgtat aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc
    10141 attttttgca gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac
    10201 tgaccgggat aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata
    10261 tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc
    10321 actaaatcat tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt
    10381 tcgaaagcga cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc
    10441 agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa tttttgaatt
    10501 aaacggtcga ttggcgcgac atgtgaaaaa tgcagtaccg gttctttttg cccgggcgta
    10561 tggcgaataa gcgtcggcag cgcagtcatt gggcgattac gattaggcaa aacctccgta
    10621 ccaacaatgg cattaatggt ggttgatttc cctgctttca tggtaccgac aatcgccagc
    10681 accatttcca gtcgggaaat tttacgcaac tcattattca gcatcgcgtg acgttcggcg
    10741 atattaggct gactccaggg taaagccagt tgtggcgcat cgtctccggg tacagagaga
    10801 ggcatttttt ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc
    10861 tgacttaact catacagggt ctgtgtgtac atagaaaatt
//
LOCUS       DAZFRJ010000150        10754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000150 DAZFRJ010000000
VERSION     DAZFRJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.152"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            91..804
                     /gene="yghS"
                     /locus_tag="ACHKUN_003546"
     CDS             91..804
                     /gene="yghS"
                     /locus_tag="ACHKUN_003546"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6661742.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            836..1594
                     /locus_tag="ACHKUN_003547"
     CDS             836..1594
                     /locus_tag="ACHKUN_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661743.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1640..2989
                     /locus_tag="ACHKUN_003548"
     CDS             1640..2989
                     /locus_tag="ACHKUN_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO6661744.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2989..3813
                     /locus_tag="ACHKUN_003549"
     CDS             2989..3813
                     /locus_tag="ACHKUN_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661745.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3825..4385
                     /locus_tag="ACHKUN_003550"
     CDS             3825..4385
                     /locus_tag="ACHKUN_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6661746.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4416..5486
                     /gene="lptF"
                     /locus_tag="ACHKUN_003551"
     CDS             4416..5486
                     /gene="lptF"
                     /locus_tag="ACHKUN_003551"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6661747.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5483..6562
                     /gene="lptG"
                     /locus_tag="ACHKUN_003552"
     CDS             5483..6562
                     /gene="lptG"
                     /locus_tag="ACHKUN_003552"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6661748.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6598..7770)
                     /locus_tag="ACHKUN_003553"
     CDS             complement(6598..7770)
                     /locus_tag="ACHKUN_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6661749.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQINAIIQAFATLKQ"
     gene            complement(7770..8018)
                     /locus_tag="ACHKUN_003554"
     CDS             complement(7770..8018)
                     /locus_tag="ACHKUN_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6661750.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8050..8964)
                     /locus_tag="ACHKUN_003555"
     CDS             complement(8050..8964)
                     /locus_tag="ACHKUN_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6661751.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8961..10649)
                     /locus_tag="ACHKUN_003556"
     CDS             complement(8961..10649)
                     /locus_tag="ACHKUN_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO6661752.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         2591 a         2676 c         2815 g         2672 t
ORIGIN      
        1 cacaattaac cattgtcaca ataaggttga acggatattc tgagcacgga ccacgtaata
       61 ttcaatacat tatttactgc cgggtttttc gtgaatcaac gtaatatgtc aataattaat
      121 tccacgccag tgcgtgttat tgccattgta ggatgtgatg gttcaggtaa atcgaccctc
      181 acggcaagcc tggtaaatga actggcagca agaatgccaa cagaacgcat ttatctcggg
      241 caatcgtccg ggcgaattgg cgaatggatt tcacagctcc ctgttattgg cgcacctttt
      301 gggcgttatc tgcgaagtaa agcggcacat gtgcacgaaa agccctcaac accgcctggc
      361 aatattactg cgctggttat ctatctgctt tcctgctggc gggcgtacaa gtttcgcaaa
      421 atgttgtgta aaagccagca aggctttctg ctcatcaccg accgctaccc gcaggttgaa
      481 gtgccgggtt ttcgctttga tggcccgcaa ttggcaaaaa ccacgggcgg taacggttgg
      541 ataaaaatgt taaggcagcg cgagctgaag ctgtaccaat ggatggcatc ttatttgccc
      601 gtattgttga ttcgtcttgg cattgatgaa caaaccgcgt ttgcgcgtaa acctgaccac
      661 caactggcag cgttacagga aaaaatcgcc gttacgccgc aactgacatt caatggcgca
      721 aagatccttg aactggatgg gcgacacccc gccgatgaaa ttctgcaagc gtcactacgc
      781 gcaattcacg ccgcgctttc ctgatcacca aaatggatag gggatgattt aacgaatgga
      841 tgcactacaa actcaaactg ttaatagtac aaccgcgccg cagcccaact acattccggg
      901 gctgattgcg gtggtcgggt gtgatggcac cggtaaatcc acactgacca ccgacctggt
      961 gaaatcgctg caacaacact ggcaaaccga gcggcgctat ctggggctgc tctccggcga
     1021 agacggcgac aaaatcaaac gattgccgtt ggttggcgtc tggctggaac ggcgactggc
     1081 ggccaaatcc tcgaaaaccc aaagcatgaa aaccaaatct ccggcgctat gggcggcggt
     1141 gattatgtac tgcttctcgc tgcgaagaat ggcgaatcta cgcaaggttc agcgactggc
     1201 gcaaagtggc gttctggtgg tcagcgatcg cttcccgcag gctgaaattt cgggctttta
     1261 ttatgatgga ccggggattg gcgtcgaacg tgcgaccggg aaaatcagca tgtttctggc
     1321 gcagcgcgaa cggcgtttat accaacaaat ggcgcaatat cgcccggaat taattattcg
     1381 cctgggcatt gatattgaga ctgccatctc ccgcaagcct gaccatgact atgccgagct
     1441 gcaggacaaa atcggcgtca tgtcgacgat tggctataac ggcacaaaaa ttcttgagat
     1501 tgattccaga gcgccctaca gcgaagtgct ggaacaggca cagaaggcgg tttctctggt
     1561 tgccatcgtt tctgaccgcc gaagtttaac ttaaggctgg cgggatactt tttactatca
     1621 acgataaggt caggctgaat tggcgggttt taacatcaaa cattggtttg cagatggcgc
     1681 gtttcgcacc attattcgca atagcgcctg gttaggctcc agtaatgtcg tgagcgcctt
     1741 gctgggtctg ttggcgctct cgtgtgccgg taaagggatg acgcccgcca tgtttggcgt
     1801 actggtgatt gtgcaatcgt acgccaagtc gatcagcgat tttattaagt ttcagacatg
     1861 gcaactggtg gttcagtacg gaacgccagc cttgaccaac aataatccgc agcaatttcg
     1921 caatgtcgtc tcattttcct tctcgctgga tatcgtcagc ggcgcggtgg cgattgtcgg
     1981 cggcattgcc ttactgccat tcctttccca ttcattaggt ctggatgacc aaagcttttg
     2041 gctggcggcg ctgtattgca cgctcattcc ttcaatggct tcctccacgc cgaccggcat
     2101 tctgcgtgcg gtagatcgct tcgatttaat tgctgtacag caggcgacga aaccttttct
     2161 gcgcgcagcg gggagcgtcg tagcctggta ttttgacttt ggttttgcgg gttttgttat
     2221 tgcctggtac gtgtcgaatc tggttggcgg caccatgtac tggtggtttg cagcgcgcga
     2281 attacgccgc agaaatatcc ataatgcctt caaattgaat ctgtttgagt ctgcccgaca
     2341 catcaaaggc gcgtggagtt ttgtctggtc aaccaacatt gcccactcca tctggtcggc
     2401 gcgtaactcg tgcagcacag tgctggtggg gatcgtgtta ggacccgctg ccgccgggtt
     2461 atttaaaatc gccatgacat tcttcgacgc cgccggaacg ccagcgggtt tgctgggtaa
     2521 aagtttttac ccagaggtga tgcgtttaga tccgcgcacc accagaccgt ggttgttagg
     2581 tgtgaagtcc ggtttactgg cgggcggaat cggtattctg gtggcgctcg cggtgtttat
     2641 cgtcggcaag ccgctcattt cgctggtgtt tggcgttaag tatctcgaag cgtatgacct
     2701 gattcaggtg atgctgggcg caattgtgat ttcgatgctg ggcttccctc aggagtcatt
     2761 gctattgatg gcgggaaaac agcgcgcttt tctcgtggcg caaaccatcg cctcaatcgg
     2821 ctacatcgta ttgctgttca tgttctgtca tctgtttggt gtgctgggag cagcgtttgc
     2881 ttacttcggt gggcaatgtc tggacgtggc gctctcgtta attccaacct taaaggcatt
     2941 ttttcagcgc cattccttgc tttataacgc agccggagag aaatcctgat gatgagaaaa
     3001 tatttcccgc ttgaggcgag cgagcgtcta tttgttgcga ttgaagaaga tgatgtcgtt
     3061 gatgcacagg ttagtttgcc gccgactatt gcactaagtt gtaccaccga aatcattcat
     3121 gataattacg cgttatgcct gcaattctgg ctcaatggcg ttgatcgtca ggaattactg
     3181 cgtttagttc gcaaacaagc caaaggcgat gagctgactg ctgatgagcg caaacaattt
     3241 aaatacatgc gagcgcgcta caagcacctg cgttttgcgc aacggctgta tttaaagaag
     3301 catcaggccg gatttttatt cggcaaaacg accgtttttc tggggcgttt tcaggacggc
     3361 tttcgtaacg gtaagaaaaa tattgtgtcg tattacggca acctgttacg gatttattta
     3421 agctctccgg tctggtcgtt ggtgaactat tcattacgcc atagccagtt agaaagcgta
     3481 agcagtttta ttgcctatcg gcaaaagcaa atgcataccc tgaaagagat tattgccaaa
     3541 ccgcgactaa ccggcaggga atttcatgat gtgcgaaaaa tcatcagcca acaggtttct
     3601 tattacgaca ccttaagatc gcttgatcct gaaaataaag aggcgttaca gatttcccgt
     3661 ttcctcgccg ccattaatgg cctgatggga gataaacacg acgacatggt ggcggatgat
     3721 atggaaaacc gccagtcgta tgatgcaccg ttggcgctgg atagcgatat tcgtcagcgc
     3781 ttagagctgc tgatttcgcg ttttccgcta tagccggagc aatgatgtca tctcgtttga
     3841 ttattgcgtt gattatcatg ctcttagccc cgggtgtgca ggcacacaat ttcgtcactg
     3901 gcaaaacggt tacccctgtt tatatccagg aaggcggcga gctgcttctg aatagcgacg
     3961 acgaaattca ctaccaaaag tggaacagca cacaattagc aggaaaagtc cgcattattc
     4021 agtatatcgc cgggcgtaag tcggcgaaga aaaagaactc actgttaatt aaagcggttg
     4081 aagcggcaaa ctttccgcag gatcgttttc agcccaccac cattgtgaac accgatgatg
     4141 ccatctttgg tactggctat tttgttgttg gcaaaatcga aaaaaataaa cgccgctatc
     4201 cgtgggcgca attcattatt gacggcaatg gacagggacg cgttgcctgg cgcttgccgg
     4261 agcagagttc aaccattctg gtgctgaata aagccgggca aattcagtgg gcaaaggacg
     4321 gcagcctgac gccggaagag gtagatcatg tcattgcgct ggcgcagaaa ctgatcaatg
     4381 aataaaaaat tcaaaggatt gagggcgtta gtgagatgaa gctggttgaa cactacatca
     4441 tgcgtggtac gcgccgtctg gtgctgatta tcgtcggttt tctgatcttt attttcgcca
     4501 gctactccgc acagcgttat ctgaccgaag cggcaaatgg gacattagct cttgatgttg
     4561 tgctggatat cgttttctac aaagtgctga ttgcactgga gatgttgtta cctgttggtc
     4621 tgtatgtgtc agttggcgtg acgctagggc agatgtatac cgactcggaa attaccgcta
     4681 tctctgcggc ggggggcagt ccgggacgct tgtacaaagc cgttctttat ctggcgatac
     4741 cgctaagtat ttttgtcacc cttctgtcga tgtatggtcg tccgtgggct tatacgcaga
     4801 tttatcaact ggagcagcag tcgcagtcgg agctggatgt tcgccagttg cgggcaaaga
     4861 aatttaacac taacgataac ggacgaatga tcctttcgca gacggttgat caggataata
     4921 atcgcctgac tgacgcgctg atttatactt ctactgccaa tcgaacccgc attttccgcg
     4981 cccgttcggt tgatgtggtt gacccatcac ccgagaaacc gaccgttatg ttgcacaacg
     5041 gcactgccta tcttctcgat catcaggggc gtgacgacaa cgaacagatc taccgtaatc
     5101 tgcaattaca tctgaatccg ctggatcaaa gccctaacgt caaacgcaaa gcaaaatcgg
     5161 tcacggagct ggcgcgctcc gcctttcctg ccgatcatgc cgaactgcaa tggcgacaaa
     5221 gccgtggcct gacagcattg ttgatggcgc tgctggccat ttcattaagt cgggtaaaac
     5281 cgcggcaagg gcgattttca acattattgc cactgacgtt gctgtttatt gccatttttt
     5341 atggcggcga cgtctgccgt acgctggtgg ctaacggcgc gattcccctc attcctggtt
     5401 tgtggttagt acccggactc atgctaatgg gcctgctgat gctggtcgca cgcgacttct
     5461 ctttgctgca gaaattttcc cgatgaatgt tttcagtcgc tatttaatcc gtcatctctt
     5521 tctcggtttt gccgccgccg cagggctatt gctgccgctt tttaccacct tcaacctgat
     5581 taacgaactg gatgatgtca gcccgggcgg ttatcgctgg actcaggcgg tgctggtggt
     5641 gctaatgacc ttaccgcgca cactggtcga actttcgccg tttatcgcgt tattgggtgg
     5701 tattgttgga ctggggcagt tatcgaaaaa cagtgagctt accgccattc gcagcacggg
     5761 gttttctatc ttccgtattg cactggtggt gctggttgcc gggatattgt ggactgtttc
     5821 gttaggcgcg atagatgagt gggtggcgtc gccattgcag caacaggcgt tgcaaatcaa
     5881 atcgaccgcc accgcgttgg gggaggacga tgacattacc ggcaatatgc tgtgggccag
     5941 gcgtggtaat gaatttgtga cggtgaaatc gctgaacgag cagggccagc cggtgggcgt
     6001 ggagatattt cattatcgcg acgatctctc gcttgaatcc tacatttatg cacgcagtgc
     6061 caccattaaa gacgacaaaa cgtgggtcct gcatggtgtg aatcataaaa aatggctgaa
     6121 tggcaaagaa acgctggaaa cattagataa tcttgcctgg caatcggcct tcaccagtat
     6181 gaatcttgaa gagttatcaa tgccggggaa tactttttct gtccgtcagc ttaatcatta
     6241 catccattat ttgcaggaaa ccgggcaacc cagcagcgaa taccgccttg cactgtggga
     6301 aaaactgggg caacccatcc tgaccctggc gatgattttg ctggctgtgc cgtttacctt
     6361 tactgccccg cgttcgcctg ggatgggtag ccgtctcgct gtaggcgtca tcgtgggctt
     6421 actcacctgg atcagctatc aaatcatggt taatctggga ttgctatttg cgttaagcgc
     6481 acctgttacc gcgctcggtt taccggtagc gtttgtgttg gtggcgttga gcctggtgta
     6541 ttggtatgac agacaacatt aaacccgccg tggcgcaaac accacggcgg gctcacatta
     6601 ctgcttcaga gtagcaaatg cctgaatgat ggcattgatt tgctcacgac tgtgggcagc
     6661 atttacgcta cagcgcagca gcgtgatgcc tgcaggcgcg gcaggcggca ggataagatt
     6721 cacatacacg cccagcgaga taagctcgcg ccagatgcgt aaaccttcct ctttcgaacc
     6781 gattattact ggcactaccg ggctaatatg tgaacttaac tcataaccca gtttttgcag
     6841 accgtcatac agatggttgg cgttatccat cagcttttgc cgcagctctg gttgtgtggc
     6901 aatagttctt aacgacgagc gcacggtcgc aatgcaggac ggagaaggag aggcggtaaa
     6961 aatatacggg cggctgctgt agcgcagcac ttccatcgct tccgaaccga cagcaaaacc
     7021 gccaatcgag gccagacttt tactgaaagt accaacgata atatccacat catcttcaac
     7081 gccgacagct tcagccagtc cgcgccccgt tgcgcctaac acgccaaacg aatgcgcttc
     7141 atcgacaatc aaataaccac cgagacgacg tttgatatca acaatttccg ccagcggcgc
     7201 aacgtcaccg agcatgctgt agatgccctc aacgatgata atcgcctctt tcgcccgttc
     7261 accgaggcga accatgcgcc gttccagatc tttagcatcg ttatggcgga aacgaataat
     7321 ttccgcgcca ccgagagaac aggcgtcata aatactggcg tggctgtcgg catccagtaa
     7381 caccacggcg ttatggtcgg caagtgcgct gatgacgcct aaattagcgg tataaccggt
     7441 tgaaaaaacg atagcggtag ggcgattgaa aaactcggca atctctttct ctaacgccag
     7501 gtgcggcgca taactgccgt ttgccatacg agaaccggtg gtgccggtgc cttgggccgc
     7561 taacgctgcc tgaccttcgc tgatggcatc atgattaaag gttaaaccga gataattatt
     7621 ggtcccggcc agaatcactt ttttattgcc gatccggcct tcggtggcgg aatacacttc
     7681 atcaatacag gtgccaaacg gtgttaaccc gttgtcacta aattgcagtc gctcgccagc
     7741 aaggcgtgca tatttatcgt atagccccat tatttattct ccagccatgg aagtagagtt
     7801 tccattaatt gcgcaggggt tttgacatcc agcagaatat taataggaat ggaaatatcg
     7861 aatctgtctt ccagcatcat taagagatcc atcactttga tcgactccag gccaagatcg
     7921 ttgacgagat cgctgtcggg cttaatttcc acaccgtttt cgaccaaatc ctgtaaacag
     7981 gaaaggatgt aatccattac tatttcacga tttaccatag aaacgttaac acacctttaa
     8041 acaagacaat caaaatagcc cgttgcgtaa tgcgtgttcc aggctaatcc cgggaaacca
     8101 attaatatct tcagaaatac ggttattact tgccgaccag tcggcgtggg ttaattcacg
     8161 aattttcgag cgggtcagca taggttcttt acctgccagg cgactcaacg cggtactgat
     8221 atccgcaagg caggtgagta ccggcagagg aataccaacc attcgcacgg aaccgcaacg
     8281 gacgttggcg gcaagttgct gtatgcgttg ccagtcatag ccgccagcga cgctatcgca
     8341 taattcatag gtttgcgtct gtacagtttc ggcgcttaac cactgaccca ctgcttgcgc
     8401 aaaatcggtg acgtgcagga aagagagctg tgtatctggt gcaccaagtc gtggcagcag
     8461 gccgcgcagc atccagtcaa acagcggttt taactcttta tcgccgggac catacacggc
     8521 tgtcgggcga aaaacgccca gcgtaatttc gtcagccatt gcagtcagcc gttgttcggc
     8581 gatgtgtttg gaatttgcgt accaggagag ctcgggatgg cgcgccgcca gcgaagagat
     8641 aaacagaaaa cgttggcaaa agcagctctc ttttgctgct tgcatcaggc gcaggctgcc
     8701 gtcaacgtta cagcgggtga aaatctcttc tttgtgcccg cgcacttgcc cggcgcaatg
     8761 gaccactgcg ctggctccgg caaccagctt gctaagtgaa tgagtatctt ccagcgaacc
     8821 gcgcacccag gtgagattat cattgacgtg agcgcgggca gtacgcgtca atgcgcgaac
     8881 gtgaaagccg cgggcgagca ggttatcgat aatatattta ccgataaacc cggtagcgcc
     8941 cgtcaccgcg acagtttgat tcatgccagg gattcctgca cattaagact ggcagcataa
     9001 gccttctgat aacgtttttt cgcttccgca cgggcaggct tgccggagga cgttcgggga
     9061 atactgtggg gcggcaacag atcgatagcc gcggtcacgc caaattcgct ttggatccgt
     9121 gccgccagcg cgtggataag ctgcccgcga cgttcttcgt cgctgatccg acactggatc
     9181 tgcaaaatga ttttttcctg ggcggtaaca aaagcaatcg catcgccaga atgaatttcc
     9241 ggttcttgtt ccgcaatata ttcaatatcc tgcggccaga tattacggcc acgaataata
     9301 atcagatctt taatgcgtcc ggtgacatac agataaccgt ccagcagata accgaggtcg
     9361 ccggtgtcta accagcccgt cgcggcaatc tcgtcttgcg aaacctggtc gccaaagtaa
     9421 ccgctcatca ggctggggcc ggagatgcaa atatggccta ccacacgttc cgcgaccggc
     9481 atacctgctt cattgcggat ttcaatgcca tgttccggca acgctttgcc gcagttgacg
     9541 aaagtcgata cggcgcgtgt ctctgcaccc ggcgcgacgg ctttaccctg atattcgagg
     9601 atgtcgcgat ccacttcgtt aaccacaacc ccggaggctt catcagagaa gctgacagcc
     9661 agcgcatttt ctgccagtcc gtagcacggc atgaaagttt tattgtcaaa gttaacctga
     9721 cggaaacatt cagcgaattg atggagttgt tctgcggaga tgggttctgc accaatacca
     9781 gcgacgcgcc agcaggaaag atccagttca gcgagatctt tttcattcac gcggcgctgg
     9841 cacaattcat agccaaacgg cggcgcaacg gaaacggtgc cgcgattttt actgatcaat
     9901 ttaagccatt gcagaggacg catggcaaaa tcctgagtgc gcaaataatc tactgaaagc
     9961 tgcgtggcga cggggggcag gagaaagccg accagtccca tatcatggta gaaaggcagc
    10021 caggagacgc agcggtcgcc agggcgtaat ttaatgccgt cgtggcttat agcacgtaga
    10081 ttagccatca cttcgcgatg ggtgataatg acgccacggg gaaaacgggt gctgccggag
    10141 gtgtactgga ggtaggcgat atcgttcgga actggacgct ggagcgcaac atcggcttcc
    10201 ggtaatgcct taaaccaggc gtggcttaaa acatgtaatt cggggttgtt atgcgtcgcg
    10261 gcattgacca gtggcaacca ctcatcacca gtgataatgg ctgcgggctg gcaacttgcc
    10321 agtaaaccct ggagtttggc gctccaggaa tcccgctgac cgacgcccat tggaatcgcc
    10381 aacgggacgg cgactaagcc agcatactga caggcaaaaa aagcctctac aaacccgcta
    10441 ctcgtttcgg caatcagcgc cacgcgttcg ccttttttca ggttcagcga taacaaccgc
    10501 ttcgcaccag cttcggcacg cgtttttaat gtctgatatt ccagttgatc ttcaagttgg
    10561 caacgtctgt cataaaaatt cattccggcg ctactcagag cggcgtagtc caaagcatca
    10621 accagcgttg gaaaatcggc atagcgcata ggtagggaat gcgtaaagat tttattagac
    10681 atatacacca cgaaaaaatt ggatatcaaa aataaaatga caataatgcg gtggattata
    10741 taggcataaa agat
//
LOCUS       DAZFRJ010000151        10536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000151 DAZFRJ010000000
VERSION     DAZFRJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.153"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..1466
                     /gene="dcuC"
                     /locus_tag="ACHKUN_003557"
     CDS             99..1466
                     /gene="dcuC"
                     /locus_tag="ACHKUN_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6661753.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1509..2006)
                     /gene="sspB"
                     /locus_tag="ACHKUN_003558"
     CDS             complement(1509..2006)
                     /gene="sspB"
                     /locus_tag="ACHKUN_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO6661754.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2012..2650)
                     /gene="sspA"
                     /locus_tag="ACHKUN_003559"
     CDS             complement(2012..2650)
                     /gene="sspA"
                     /locus_tag="ACHKUN_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO6661755.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3045..3437)
                     /gene="rpsI"
                     /locus_tag="ACHKUN_003560"
     CDS             complement(3045..3437)
                     /gene="rpsI"
                     /locus_tag="ACHKUN_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO6661756.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3453..3881)
                     /gene="rplM"
                     /locus_tag="ACHKUN_003561"
     CDS             complement(3453..3881)
                     /gene="rplM"
                     /locus_tag="ACHKUN_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO6661757.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4100..5227)
                     /gene="zapE"
                     /locus_tag="ACHKUN_003562"
     CDS             complement(4100..5227)
                     /gene="zapE"
                     /locus_tag="ACHKUN_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO6661758.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5421..5819
                     /gene="zapG"
                     /locus_tag="ACHKUN_003563"
     CDS             5421..5819
                     /gene="zapG"
                     /locus_tag="ACHKUN_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO6661759.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5973..7340
                     /gene="degQ"
                     /locus_tag="ACHKUN_003564"
     CDS             5973..7340
                     /gene="degQ"
                     /locus_tag="ACHKUN_003564"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO6661760.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7430..8497
                     /gene="degS"
                     /locus_tag="ACHKUN_003565"
     CDS             7430..8497
                     /gene="degS"
                     /locus_tag="ACHKUN_003565"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO6661761.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8560..9498)
                     /gene="mdh"
                     /locus_tag="ACHKUN_003566"
     CDS             complement(8560..9498)
                     /gene="mdh"
                     /locus_tag="ACHKUN_003566"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6661762.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9933..10403
                     /gene="argR"
                     /locus_tag="ACHKUN_003567"
     CDS             9933..10403
                     /gene="argR"
                     /locus_tag="ACHKUN_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO6661763.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2581 a         2679 c         2630 g         2646 t
ORIGIN      
        1 catccgcacc ataaatagac ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt
       61 atgggattga ccgttttctt ttgacacgga gttcaacaat gttcggcata attatatctg
      121 tcatcgtatt aattacgatg ggctatttga tcctgaaaaa ctacaaacct caggtggtgc
      181 tggctgccgc aggtatcttc ctgatgatgt gcggtgtctg gttagggttc ggtggtgtac
      241 tcgatcccgc caaaagcagc ggctacttga tcgtcgatat ttataatgaa atcctgcgca
      301 tgctgtccaa ccgcattgcc ggattggggc tgtcgattat ggcagtgggc ggttatgccc
      361 gctacatgga gcgcacagga gccagtcgcg cgatggtgag cttgctaagc cgcccgttaa
      421 aactcattcg ctcgccgtat attattctgt cggcaactta cgtcatcggc caaatcatgg
      481 cgcagtttat taccagcgcc tccggcctgg gtatgttgct gatggtcacc ttatttccga
      541 cgctggtgag tctgggagta agtcgcctct ctgcggtggc ggttatcgca accacgatgt
      601 ccattgagtg ggggattctg gaaacgaact ccatttttgc agcccaggtc gcgggaatga
      661 aaattgccac ttacttcttc cactaccagc ttccggtcgc ctcttgcgtc attatctcgg
      721 tggcgatctc ccactttttc gtgcaacgcg cttttgacaa aaaagataaa aatatcaatc
      781 acgaacaggc agagctaaaa gctctcgata atgtcccgcc gctctattac gccattttac
      841 ctgtgatgcc gttaatcttg atgctcggct cgctgttcct cgcccacatc gggctgatgc
      901 agtcagaact gcatctggtg gcggtgatgt tactgagttt gacggtgacg atgtttgttg
      961 agttcttccg caagcataac ttgcgcgaaa caatggacga tgtgcaggcg ttttttgacg
     1021 gcatgggtac gcagtttgcc aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga
     1081 aaggcttaac gacgattggc actgttgatg cggttatcag gggtgcggag cattctggtc
     1141 tgggcggtat tggcgtgatg attattatgg cgctagtcat tgccatttgt gccattgtga
     1201 tgggctctgg caatgcgccg tttatgtcat ttgccagtct tattccgaat atcgcagccg
     1261 gactacatgt accagcggtt gtaatgatta tgccgatgca ttttgccacg acgctagcgc
     1321 gcgcggtttc gccgattact gcggtggtgg tcgttacgtc aggaattgca ggcgtttcgc
     1381 cttttgcggt ggtgaagcga acagcgatcc ccatggcagt cggtttcgtg gtgaatatga
     1441 ttgccacaat cacgctattt tattaagcca ttaaaagaca aaacaggcca ccagggcctg
     1501 tttttgtatt acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt
     1561 tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata
     1621 acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc ttcatcgtag
     1681 gcagcttcag gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg
     1741 gcagccagcg gcacagaaac ctgacgcgga atgccgccaa agcgcgcgtt aaagcgtacc
     1801 tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc
     1861 ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc caccaccaag
     1921 tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga
     1981 cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt
     2041 ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt
     2101 tcagctcttt cgcacccggg ccgctgaact cgatgcccag ttgcggcaga cgccacagca
     2161 gcggagcaag atagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac
     2221 cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatctgctt
     2281 cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat
     2341 gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg
     2401 ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat
     2461 ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt
     2521 ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct
     2581 gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag
     2641 cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg
     2701 gccagtcaga agttgtgtta cccagtaagg aacgactctc tttgttcgaa aatcaaacaa
     2761 aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg
     2821 acctttgtgg tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata
     2881 gttgacttag tcccttattg gcgatgtggt ttttgttttg cctgtctgtc agacggtagc
     2941 aaaaagcaac tttccaattt ttacgctgat tcagaattta accataaaaa aacccgccga
     3001 agcgggtttt ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac
     3061 tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca
     3121 cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga
     3181 gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg
     3241 gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc
     3301 atgcgagcag tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta
     3361 ccgttgcccg gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc agtgccgtag
     3421 tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt
     3481 gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc
     3541 agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga
     3601 gcaatcatct cttcaaaggt cgcttgtttg ataccaccga tgtggccggt gtggtgatag
     3661 tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg
     3721 atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga
     3781 cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag
     3841 tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa
     3901 tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc
     3961 cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg ggaaaaaaac
     4021 tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt
     4081 ttgtgatttg tgacaggttt taacccgcca aatgctcgcg cttcagatac tcttcgcttt
     4141 gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga tcgccctgat
     4201 aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact
     4261 catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta
     4321 catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca tgctgactgc
     4381 gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg acgcccattg
     4441 tcgccaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc gcccccgcca
     4501 gcgccaacca tagtttatcc atctgcgccc gggtttcatc gttgagtggc gaaagccaca
     4561 gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg ttcattacat
     4621 cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc aggccatttc
     4681 gataaagttc atccggcgga atatttgacg tcgctaccag ggtaatgccg cgggcgaaca
     4741 gggctttcat cagaccgcca agtagcatgg cgtcggtaat atcagaaaca aaaaattcgt
     4801 caaaacagag cacgtcagtt tcggctttaa agcgatcggc aataatttcc agcggatcgg
     4861 tctgcccctg taaggcagtt agctcttcgt gcacccgcag cataaaacgg tgaaagtgca
     4921 ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc caggttttcc
     4981 cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt gtgtcttcgc
     5041 gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcc
     5101 tgctattgat gagttcttga taaataattt ccaggcggct gacggcctct ttttgaacgt
     5161 cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc
     5221 tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga
     5281 aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg tggaattaac
     5341 ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca
     5401 aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca
     5461 tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt
     5521 tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta
     5581 gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt
     5641 atcagcacat ggcgaaaagc tccagcagcc tgctgccgga actgtctgct gaagcaaacc
     5701 cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc
     5761 ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat
     5821 ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa
     5881 tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct
     5941 tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaacccag ctgttgagtg
     6001 cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc
     6061 caggccaggt tgccgatcag gcccctctcc ccagtctggc tccaatgctg gaaaaagtgc
     6121 ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg
     6181 aagaattcaa aaagtttttt ggtgatgatt taccggatca acctgcacaa cccttcgaag
     6241 gtttaggttc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg
     6301 tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa
     6361 aactgattgg tagcgatgac cagagcgata tcgccctgtt acaaattcaa aacccgagca
     6421 aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtag
     6481 cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt tccgcattag
     6541 gccgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat gcttccatta
     6601 accgcggtaa ctccggcggt gcactattaa accttaacgg tgagttaatt ggcatcaaca
     6661 ctgcaatcct tgcgcctggc ggcgggagcg tcgggattgg atttgccatc cccagtaata
     6721 tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc ggtttgttag
     6781 gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc
     6841 gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcaaaagcg ggcgtcaaag
     6901 cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct
     6961 ctcgtatcgc gactacagag ccgggcacga aagtgaagct tggcctgctg cgtaacggca
     7021 aaccactgga agtagaagtg acgcttgata ccagcacctc ttcgtcggcc agcgctgaaa
     7081 tgatcacgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca
     7141 aaggtattaa gatcgatgaa gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa
     7201 aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta
     7261 aagtgctggc ggcaaaaccg gccatcatcg ccctgcaaat tgtacgcggt aatgaaagca
     7321 tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt gatgtccggt
     7381 taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca tgtttgtgaa
     7441 gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc
     7501 ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg atgagacgcc
     7561 tgccagttat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg
     7621 tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat ccggtgtaat
     7681 catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg ccgatcagat
     7741 catcgtcgcc ttacaggatg gacgtgtatt tgaagcattg ctggtgggat ctgactctct
     7801 aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca ttccgatcaa
     7861 tgcacgtcgc gtaccgcaca ttggcgacgt ggtactggcg atcggtaacc cgtacaacct
     7921 cgggcagacc attacccagg ggattattag tgctacgggt cgaatcggtc tgaacccgac
     7981 cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact ctggcggcgc
     8041 gctggtgaac tcgctgggag aactgatggg cattaacacg ctgtcgtttg ataagagtaa
     8101 cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag caaccaaaat
     8161 tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggta tcggcggacg
     8221 cgagatcgca ccactgcacg cgcagggcgg tggtatagat caactgcaag ggatcgtggt
     8281 taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca acgatctgat
     8341 tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc aggtggcaga
     8401 aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc agttaacgct
     8461 gcaggtcacc attcaggaat atccggcaac caattaagtc gagtgctcaa aacaaaaaac
     8521 cggagtctgt gctccggttt tttattattc gctaattaat tacttattaa cgaactcttc
     8581 gcccagggcg atatctttct tcagcgtatc cagcatacct tccagtgcgc tctgttcaaa
     8641 tgcgctcagg gtaccgatag atttacgctc ttccacgccg tttttaccca gcagcagcgg
     8701 ttgagagaag aaacgggcgt actgaccgtc gccttcaacg taggcacatt cgacaacgcc
     8761 ttgttcgccc tgcagtgcac gaaccagaga cagaccaaaa cgtgcagctg cctggcccat
     8821 agacagggtt gcagacccac caccggcttt cgcttcaacc acttcagtac ctgcgttctg
     8881 gatacgtttg gtcagatcag ccacttcctg ctcggtaaaa ctaacgccag gaacctgtga
     8941 cagcagcggc agaatggtaa caccagagtg accgccaata accggcactt caacttcgcc
     9001 tggctgtttg cctttcagtt ccgcaacaaa agtgttggaa cgaatgatat ccagcgtggt
     9061 aacgccgaac agtttgtttt tgtcataaac accggctttt ttcagcactt cagcagcaat
     9121 cgcaactgtg gtgttaaccg ggttagtgat aataccaatg cacgctttcg ggcaggtttt
     9181 cgcaacttgc tgtaccaggt ttttcacgat gccggcgtta acgttaaaca ggtcggaacg
     9241 atccataccc ggtttacgcg ctacacctgc agagataaga acgacatctg cgccttccag
     9301 cgccggagtc gcatcttcac cagaaaaacc tttgattttc acagcagtag ggatatggct
     9361 cagatcgaca gccacaccgg gagtcactgg agcgatatca tacagagaga gttctgaacc
     9421 tgaaggcagt tgggttttta acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc
     9481 gaggactgcg actttcatcc taaactcctt attatattga taaactaaga tatgttgctc
     9541 cgctgccgcg accttaatcc acaaaattgc cgtttacaat gaccacatct caagaatgtg
     9601 tagtcacgca agtttagcgt ttatgcattt aattgccgta atcaggaacc taacttacgt
     9661 aattaacagt cgcttatgca ttagcgcaac attccaacag gtggtgacaa tataccctac
     9721 cgttcagcca aaacaacatc aatttgataa caattaattt acttttaaac agaatttgca
     9781 tgccgtgacg caggcatgtt tctcaataac gaaatttgat aaaatcccgc tctttcataa
     9841 cattatttca gccttcttca gggctgactg tttgcataaa aattcatctg tatgcacaat
     9901 aatgttgtat caaccaccat atcgggtgac ttatgcgaag ctcggctaag caagaagaac
     9961 tagttaaagc atttaaagca ttacttaaag aagagaaatt tagctcccag ggcgaaatcg
    10021 tcgccgcgtt gcaggagcaa ggctttgaca atattaatca gtctaaagtc tcgcggatgt
    10081 tgaccaagtt tggtgctgta cgtacacgca atgccaaaat ggaaatggtt tactgcctgc
    10141 cagctgaact gggtgtacca actacctcca gtccattgaa gaatctggtg ctggatatcg
    10201 actacaacga tgcagttgtc gtgattcata ccagccctgg cgcggcgcag ttaattgctc
    10261 gcctgctgga ctcactgggc aaagcagaag gtattctggg caccatcgct ggcgatgaca
    10321 ccatctttac cacccctgct aacggtttca ccgtcaaaga cctgtacgaa gcaattttag
    10381 agctgttcga ccaggagctt taatctctgc cccgtcgctt ctggcgtcgg ggaaaatgtt
    10441 gcttatccct ctcattcccc ttctttctcc tgcgatacat ttaacgaata gtgcgtttta
    10501 ccgcgacaca tcattcacaa aatgaataca tacggt
//
LOCUS       DAZFRJ010000152        10389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000152 DAZFRJ010000000
VERSION     DAZFRJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.154"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            100..1242
                     /gene="yghO"
                     /locus_tag="ACHKUN_003568"
     CDS             100..1242
                     /gene="yghO"
                     /locus_tag="ACHKUN_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO6661764.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1249..2013)
                     /gene="glcC"
                     /locus_tag="ACHKUN_003569"
     CDS             complement(1249..2013)
                     /gene="glcC"
                     /locus_tag="ACHKUN_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO6661765.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2264..3763
                     /gene="glcD"
                     /locus_tag="ACHKUN_003570"
     CDS             2264..3763
                     /gene="glcD"
                     /locus_tag="ACHKUN_003570"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO6661766.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3763..4815
                     /gene="glcE"
                     /locus_tag="ACHKUN_003571"
     CDS             3763..4815
                     /gene="glcE"
                     /locus_tag="ACHKUN_003571"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO6661767.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4826..6049
                     /gene="glcF"
                     /locus_tag="ACHKUN_003572"
     CDS             4826..6049
                     /gene="glcF"
                     /locus_tag="ACHKUN_003572"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO6661768.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6054..6458
                     /gene="glcG"
                     /locus_tag="ACHKUN_003573"
     CDS             6054..6458
                     /gene="glcG"
                     /locus_tag="ACHKUN_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO6661769.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6480..8651
                     /gene="glcB"
                     /locus_tag="ACHKUN_003574"
     CDS             6480..8651
                     /gene="glcB"
                     /locus_tag="ACHKUN_003574"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO6661770.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8879..9124
                     /gene="traT"
                     /locus_tag="ACHKUN_003575"
                     /pseudo
     CDS             8879..9124
                     /gene="traT"
                     /locus_tag="ACHKUN_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9157..>10389)
                     /gene="cptA"
                     /locus_tag="ACHKUN_003576"
     CDS             complement(9157..>10389)
                     /gene="cptA"
                     /locus_tag="ACHKUN_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6661771.1"
                     /translation="NTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNR
                     LLEENNALPALKNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTE
                     QNMFVFNNVVSSRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQ
                     QTMTARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIH
                     LLGTHIKYKYRYPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKT
                     FKATAPDGFLFYFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRH
                     HTFSEYINRKYSLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNAL
                     TDYDTLPYAELRVNQASN"
BASE COUNT         2534 a         2554 c         2872 g         2429 t
ORIGIN      
        1 tttattcttt gtttgtattc attgggttga ttattaaggt gtcgttcgtt gctgttgcaa
       61 cctgtttttc accgttaatt atttgttatc cacagagata tggaatgcga tctgctgatg
      121 attaaaattg aaaaagtaat taataaaaat gaccttaagg catttatcgc ttttccgtca
      181 tcactttatc ccgacgatcc aaactggata ccccctttat tcatagaacg caacgagcat
      241 ttgtctgcga aaaatccggg gacggaccat atcatctggc aggcgtgggt ggcaaaaaaa
      301 gcggggcaga tagttgggcg tattaccgcg caaatagata ccttgcaccg tgagcgttac
      361 ggtgaagata ccggtcattt cggcatgatt gacgccattg atgatccgca ggtttttgcc
      421 gcgttgtttg gcgcagcgga agcgtggttg aagtcacaag gtgcaagtaa gatcagcggt
      481 cctttcagcc tgaatatcaa tcaggaaagc ggattactga ttgaaggttt tgacacacca
      541 ccctgtgcga tgatgccaca cggcaaaccg tggtatgccg cgcatattga acaactgggt
      601 tatcacaaag gcattgattt actggcgtgg tggatgcagc gaaccgatct cactttctct
      661 ccggcgctaa aaaaactgat ggatcaggtg cgcaaaaagg tgaccattcg ctgcatcaat
      721 cgtcagcggt ttgccgaaga aatgcagatc ctgcgtgaga ttttcaactc tggctggcag
      781 cacaactggg gatttgtgcc gtttaccgaa catgaattcg cgaccatggg cgatcaactt
      841 aaatatctgg tgccggacga tatgatctat atcgctgaga ttgattctgc accctgcgcg
      901 tttattgtcg gcttgccgaa catcaacgag gcgattgccg atctgaacgg atcgctcttt
      961 cccttcggct gggcaaaatt gctgtggcgt ttgaaagtca gcggtgtgcg aactgcgcga
     1021 gtaccgctga tgggcgtgcg cgacgagtat cagttcagcc gcatcggccc ggtgattgcg
     1081 ctgttattga ttgaagcctt acgcgatccg tttgcccgcc ggaagatcga tgcgctggag
     1141 atgtcatgga tcctcgaaac caatacaggc atgaataaca tgctggaacg cattggggcg
     1201 gagccgtaca agcgttaccg attgtatgaa aagcagattt aataccagct aactcaggtt
     1261 catctccagc ggcaccgccg agcgaatcaa atggtggcct tccagctcga tatcgtggag
     1321 attctttttc acggtccgca catgatcgcg tgctgcgcgc tgggcgacgt gcggcagccg
     1381 ctgcaacacc gcgttgtaga tcctcgcatg ctggcgatcg atctgctttt tttgctgtgg
     1441 tcgatggtag agattattta cgctggcaaa cactgaatta aacatcagat cggtcaatga
     1501 ttgcagcgta aacaccagca cctgattgtg agaagcctga caaatggcga gatggaaagc
     1561 gtgatccaac tgcgcatgtt cgatcagcga aatctctttg ttgttctcac tggcggcgag
     1621 cattttttca taacagcggg ttatcacaac aaaatcagcc tgcgttccca gcgttgccgc
     1681 cagccttgcc gattcgccct ccagtaatgc gcgaacgtcg agcagatcgt acagcgttcg
     1741 cggctgcgta ctgaacagat ggatcagcgg actggtgtcc tgcacccgat taagccgtgc
     1801 gacacgagaa tcgcgaccct gcgccgtttc aataatcccg cgcccgcgca gcacggtcag
     1861 cccttcacgc agtgcggagc gtgagaagcc gagcttttca cacagtcgac gttccgaggg
     1921 aagtggctga ccgaccttca gtacgccgtc gataattaac cgttcgatac tctctgcaac
     1981 cacttcgcaa atagggcgac gttcatcttt cattcccgga cctcgtgcac aggtaggacc
     2041 aatttttctg ttcttaactc gcaaaacacg cacatcacgt aagtgattat gttacatcaa
     2101 tttaacattg agttaaccaa ggcaaggtca cagagctgga aaaaaaatgg tctgaccggt
     2161 agctaaagag atagacgaaa acgaaaagcc cgcttaataa ctgttcacag aagcagcgcg
     2221 caaaaatcag ctgccacaca acacaacaaa gcgaagccta ctcatgagca tcttgtacga
     2281 agagcgtctt gatggcgctt tacccgatgt cgaccgcaca tcggtactga tggcactgcg
     2341 tgagcatgtc cctggacttg agatcctgca taccgatgag gagatcattc cttacgagtg
     2401 tgacgggttg agcgcgtatc gcacgcgtcc attactggtt gttctgccta agcaaatgga
     2461 acaggtgaca gcgattcttg ctgtctgcca tcgcctgcgt gtaccggtgg tgacccgtgg
     2521 tgcaggcacc gggctttctg gtggcgcgct gccgctggaa aaaggtgtgt tgttggtgat
     2581 ggcgcgcttt aaagagatcc tcgacattaa ccccgttggt cgccgcgcgc gcgtgcagcc
     2641 aggcgtgcgt aacctggcga tctcccaggc cgttgcaccg cataatctct actacgcacc
     2701 ggacccttcc tcacaaatcg cctgttccat tggcggcaac gtggcagaaa atgccggcgg
     2761 cgtccactgc ctgaaatatg gtctgaccgt acataacctg ctgaaaattg aagtgcaaac
     2821 gctggacggc gaggcgctga cgctgggatc ggacgcgctg gattcacctg gttttgacct
     2881 gctggcgctg ttcaccggat cggaaggtat gctcggcgtg accaccgaag tgacggtaaa
     2941 actgctgccg aagccgcccg tggcgcgggt tctgttagcc agctttgact cggtagaaaa
     3001 agccggactt gcagttggtg acatcatcgc caatggcatt atccccggcg ggctggagat
     3061 gatggataac ctgtcgatcc gcgcggcgga agattttatt catgccggtt atcccgtcga
     3121 cgccgaagcg attttgttat gcgagctgga cggcgtggag tctgacgtac aggaagactg
     3181 cgagcgggtt aacgacatct tgttgaaagc gggcgcgact gacgtccgtc tggcacagga
     3241 cgaagcagag cgcgtacgtt tctgggccgg tcgcaaaaat gcgttcccgg cggtaggacg
     3301 tatctccccg gattactact gcatggatgg caccatcccg cgtcgcgccc tgcctggcgt
     3361 actggaaggc attgcccgtt tatcgcagca atatgattta cgtgttgcca acgtctttca
     3421 tgccggagat ggcaacatgc acccgttaat ccttttcgat gccaacgaac ccggtgaatt
     3481 tgcccgcgcg gaagagctgg gtgggaagat cctcgaactc tgcgttgaag ttggcggcag
     3541 catcagtggg gaacatggca tcgggcgaga aaaaatcaat caaatgtgcg cccagttcaa
     3601 cagcgatgaa atcacgacct tccatgcggt caaggcggcg tttgaccccg atggtttgct
     3661 gaaccctggg aaaaacattc ccacgctaca ccgctgtgct gaatttggtg ccatgcatgt
     3721 gcatcacggt catttacctt tccctgaact ggagcgtttc tgatgctacg cgagtgtgat
     3781 tacagccagg cgctgctgga gcaggtgaat caggcaatta gcgataaaac gccgctggtg
     3841 attcagggca gcaatagcaa agccttttta ggtcgccctg tcaccgggca aacgctggat
     3901 gtgcgttgtc atcgcggcat tgttaattac gacccgaccg agctggtgat aaccgcgcgt
     3961 gccggaacgc cgctggtggc gattgaagcg gcgctggaga gcgccgggca aatgctcccc
     4021 tgcgagccgc cgcattatgg tgaagaagcc acctggggcg ggatggtcgc ctgcgggctg
     4081 gcggggccgc gtcgcccgtg gagcggttcg gtccgcgatt ttgtcctcgg cacgcgcatc
     4141 attaccggcg ctggaaaaca tctgcgtttt ggtggcgaag tgatgaaaaa cgttgccgga
     4201 tacgatctct cacggttaat ggccggaagc tacggttgtc ttggcgtgct cactgaaatc
     4261 tcgatgaaag tgttaccgcg accgcgcgcc tccctgagcc tgcgtcggga aatcagcctg
     4321 caagaagcca ttaacgaaat cgcccagtgg caactccagc cgctacccat tagtggctta
     4381 tgttacttcg acaatgcgtt gtggatccgc cttgagggcg gcgaaggatc ggtaaaagcg
     4441 gcgcgtgaac tgctgggggg cgaagaggtt gcgggtcagt tctggcagca attgcgtgaa
     4501 caacaactgc cgttcttctc gttaccaggt accttatggc gcatttcatt acccagcgat
     4561 gcgccgatga tggatttacc cggcgagcaa ctgatcgact ggggcggagc gttacgctgg
     4621 ctgaaatcga cagccgagga caatcaaatc catcgcatcg cccgcaacgc tggcggtcat
     4681 gcgacccgct ttagtgccgg agatggtggc tttgccccgc tatcggctcc tttattccgc
     4741 tatcaccagc agcttaaaca gcagctcgac ccttgcggcg tgtttaaccc cggtcgcatg
     4801 tacgcggaac tttgaggagc aggctatgca aacccaatta actgaagaga tgcggcagaa
     4861 cgcgcgcgcg ctggaagccg acagcatcct gcgcgcctgt gttcactgcg gattttgtac
     4921 cgcaacctgc ccaacctatc agcttctggg cgatgaactg gacgggccgc gcgggcgcat
     4981 ctatctgatt aaacaggtgc tggaaggcaa cgaagtcacg cttaaaacac aggagcatct
     5041 cgatcgctgc ctcacttgcc gcaattgtga aaccacctgt ccttctggtg tgcgctatca
     5101 caatttgctg gatatcgggc gtgatattgt cgagcagaaa gtgaaacgcc cactgccgga
     5161 gcgaatgctg cgcgaaggat tgcgccaggt agtgccgcgt ccggcggtct tccgtgcgct
     5221 gacgcaggta gggctggtgc tgcgaccgtt tttaccggaa caggtcagag caaaactgcc
     5281 tgctgaaacg gtgaaagcta aaccgcgtcc gccgctgcgc cataagcgtc gggttttaat
     5341 gctggaaggc tgcgcccagc ctacgctttc gcccaacacc aacgcggcaa ctgcgcgagt
     5401 gctggatcgt ctggggatca gcgtcatgtc agctaacgaa gcaggctgtt gtggcgcggt
     5461 ggactatcat cttaatgcgc aggagaaagg gctggcacgg gcgcgcaata atattgatgc
     5521 ctggtggccc gcgattgaag caggtgccga ggcaattttg caaaccgcca gcggttgcgg
     5581 cgcgtttgtc aaagagtacg ggcagatgct gaaaaacgat gcgttatatg ccgataaagc
     5641 gcgtcaagtc agtgaactgg cggtcgattt agtcgagctt ctgcgcgagg aaccgctgga
     5701 aaaactggca gttcgcggcg ataaaaaact ggccttccac tgcccgtgta ccctacaaca
     5761 tgcgcaaaag ctgaacggcg aagtggaaaa agtgttgctt cgtcttggat ttaccttaac
     5821 ggacgttccc gacagccatc tgtgctgcgg ttcagcggga acatatgcgt taacgcatcc
     5881 cgatctggca cgccagctgc gggataacaa aatgaatgcg ctggaaagcg gcaaaccgga
     5941 aatgatcgtc accgccaaca ttggttgcca gacgcatctg gcgagcgccg gtcgtacctc
     6001 agtgcgtcac tggattgaaa ttgtagaaca agcccttgaa aaggaataaa aaaatgaaaa
     6061 ctaaagtcat tcttagccag caaatggcga gtgcaattat tgccgcaggt caggaagagg
     6121 cgcagaaaaa taactggtcg gtttccattg ctgttgccga tgacggcggt catctgctgg
     6181 cgttaagtcg catggacgat tgcgcgccga ttgcggctta catctcccag gagaaagcgc
     6241 gtaccgccgc gctggggcgt cgtgaaacta agggctatga agagatggtg aacaacggac
     6301 gtaccgcgtt cgtgactgcg ccgttactga cgtcgctgga aggcggcgta ccggttgttg
     6361 tggatgggca aattattggt gccgtgggcg tttctggttt aaccggagca caggatgcgc
     6421 aggtcgcgaa agcggcagca gcggtgttgg cgaaataagc gaaaacgagg agagaaacaa
     6481 tgagtcaaac cataacccag ggccgtttac gcattgacgc caattttaaa cgttttgtgg
     6541 atgaagaagt tttaccggga acagggctgg acgctgcggc gttctggcgc aattttgatg
     6601 agatcgttca cgatctggca ccagaaaatc gtcagttgct ggcagaacgc gatcgcattc
     6661 aggcagcgct tgatgagtgg catcgcagca atccggggcc ggtaaaagat aaagcggcct
     6721 ataaatcttt cctgcgtgaa ctgggctacc tggtgccgca accggagtgc gtgacggtgg
     6781 aaaccacggg cattgacagc gaaatcacca gtcaggcagg gccacagctg gtggttccgg
     6841 caatgaacgc ccgctacgcg ctgaacgcgg cgaacgctcg ctggggctca ctgtacgatg
     6901 cgttatacgg cagcgacatc atcccgcagg aaggggcgat ggtcagcggc tacgatccgc
     6961 agcgcggtga gcaggttatc gcctgggttc ggcgtttcct cgatgaatct ctaccgctgg
     7021 aaaacggcag ctatcaggat gtggtggcgt ttcaggtggt cgataaacaa ttacgcatcc
     7081 agttgaaaaa tggtaaagaa accacgttac gtaccccggc gcagtttgtc ggttaccgtg
     7141 gcgatgccgc tgcgccgacc tgcattttgc tgaaaaataa cggcctgcat attgaactgc
     7201 aaattgatgc caacggacgg attgggaaag acgatgcggc gcacatcaac gatgttatcg
     7261 tcgaagctgc aatcagtacc attctcgact gcgaagattc ggtcgcggcg gttgatgcgg
     7321 aagataaaat cctgctgtac cgcaatctgc tgggcctgat gcaggggact ctccaagaga
     7381 aaatggagaa aaacggtcga caaatcgtgc gtaaactgaa tgacgatcgt cagtacaccg
     7441 ccgccgatgg ctctgaaatt tctctgcacg gacgctcgct gctgtttatc cgcaacgtgg
     7501 gtcatttgat gaccattcct gtgatttggg acagcgaagg caatgaaatc ccggaaggca
     7561 ttcttgatgg cgtcatgact ggcgcgattg ccctctatga tttaaaagtg cagaaaaact
     7621 cgcgcactgg cagcgtctat attgtgaaac cgaaaatgca cggtccgcag gaagtggcgt
     7681 tcgccaacaa actgtttacc cgcattgaga caatgctcgg tatggcaccg aataccctga
     7741 aaatgggcat tatggatgaa gaacgtcgga cctcgctgaa cttgcgtagc tgtatcgctc
     7801 aggcacgcaa ccgcgtggcg ttcatcaata ccggtttcct cgaccgtacc ggcgatgaaa
     7861 tgcattcggt gatggaagct ggcccgatgc tgcgtaaaaa tcagatgaaa tcgacgccgt
     7921 ggatcaaagc ctacgagcgt aataacgtgc tttccggcct gttctgtggg ctgcgcggta
     7981 aagcgcaaat cggtaaaggc atgtgggcaa tgccggacct gatggcagac atgtacagcc
     8041 agaagggcga ccaactgcgt gccggggcaa acacagcctg ggttccgtcg ccaactgccg
     8101 ctacgctcca tgcgatgcac taccaccaaa ccaacgtaca gagcgtgcaa gccaacattg
     8161 cccagactga gttcaatgct gaatttgaac cgctgctgga cgatctgctg actattccgg
     8221 ttgctgaaaa cgctaactgg tcggcgcaag agatccaaca agagctggat aacaacgtgc
     8281 aggggattct ggggtacgtg gtgcgctggg tggagcaggg gattggttgt tcaaaagtgc
     8341 cggatattca caatgtggcg ctgatggaag accgcgcaac gctgcgtatc tccagccagc
     8401 atatcgccaa ctggttacgt cacggtattc tgaccaaaga acaggtgcag gcgtcgctgg
     8461 agaatatggc gaaagtggtt gatcagcaaa acgctgacga tccggcttat cgtccgatgg
     8521 cggggaattt cgctaactcg tgtgctttta aagctgccag cgatttaatc ttcctcggcg
     8581 tgaaacagcc aaacggctat accgaaccgt tattacacgc ctggcgttta cgcgaaaaag
     8641 aaagtcatta atagcgtcaa aaaaagcccc ggcaagtttt attgtcgggg ctatgatagt
     8701 aactgcggta gtacatttat cccggaaata accggagcga ttatcagggt ttaacaatat
     8761 taaatacccg tagcgcatta ctagcgggcc tataacaaca atatggacat cttaacgata
     8821 ctctgcgcct gcgtgtgcag aacagaaacc cttacccgat atacttgtta tgccagaggt
     8881 agcaattgcg gcaaccgatc tgactggcat ggatgcagat gcaatggtgg aagatataaa
     8941 ttacacgata attacagatg ttcagattac cgaacgaact aaaacctatg tcagagcaga
     9001 taatgctacc acttttcggc agggaacatc aggcaccgaa attcaggcta gcacagaaac
     9061 cagcaaccag gttaatctga aatttgaagg ggctaaacct cttctggaaa tacaactgaa
     9121 ataaatatcc tccggctatg ccggaggata tttattttaa tttgaagcct gatttacacg
     9181 cagctctgcg tagggcaggg tatcatagtc agttaacgca tttttcttat aggggtttcc
     9241 tatccagcga gtgatttctt tgaaatgctg gtttaatatg gaacgctctg gctcatagtt
     9301 atcataacgt aaaccagcca aatccgacca ggtatggata aaatcagcca gactgtattt
     9361 acggttaatg tactcagaaa atgtatgatg tcgattaagt agccattttt cagaggccca
     9421 gagaataaat ggaatattgt acataggtcg ggttggggtg tcttcgttac gtcccagcat
     9481 tttatgtggt ggcgtatcga atacttcctc tccatggtca gaaaaataga aaagaaaacc
     9541 atcaggggct gttgctttga aagttttgat caacgttgca acaacatggt cattaaatat
     9601 attagcatta tcgtaatcgt tatataattc cagttcatta tcagtaagtc cttctggtgc
     9661 atgttccctg tccccatcga atactccctg accttcagga taacggtatt tatatttgat
     9721 gtgtgttccc aacaagtgaa taataacaaa ttttttggga gctggatctg ccagtacttc
     9781 actaaacgga gccagtacgt tagtatcgta ctcacgggca ctttgcgttc tctgctgatt
     9841 catgtagtac tgtttatctg tttgccgaga gaagactgtc agcattgtgt tgcgtgcagt
     9901 catcgtttgt tgattggtga tccagaatgt tttgtaaccg gcctgtttca ttatattcat
     9961 cagggaaggt tgtgtcaggt agagttcggg ctgctgttca tcagcgaaag tcaatacctg
    10021 ctgtagtact tcgatggtat atggtcttga ggagacaaca ttattaaaca caaacatatt
    10081 ttgttccgtt ttgtataatt catctagtcc gggggttgtt tttctgggat atccatacag
    10141 actcattctc ccacgctggg ttgattctcc tatcaccaga accagtgttc gtggggtatc
    10201 tccggaaata tcgttaaagt ttttcagagc ggggagcgca ttattttcct ccagcaaacg
    10261 gttcagagaa ttaagctgga gacggtactg ataatatccg gtaataaatt gccagggggc
    10321 agcgggctcc atacgggatg ccaggcgtcc cagagtattg tcaatagtcc tgtttttaac
    10381 aacagtatt
//
LOCUS       DAZFRJ010000153        10359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000153 DAZFRJ010000000
VERSION     DAZFRJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.155"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKUN_003577"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKUN_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO6661772.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKUN_003578"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKUN_003578"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO6661773.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKUN_003579"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKUN_003579"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO6661774.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKUN_003580"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKUN_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO6661775.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKUN_003581"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKUN_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO6661776.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKUN_003582"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKUN_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO6661777.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKUN_003583"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKUN_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO6661778.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKUN_003584"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKUN_003584"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO6661779.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKUN_003585"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKUN_003585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO6661780.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHKUN_003586"
     CDS             9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHKUN_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO6661781.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
BASE COUNT         2662 a         2738 c         2428 g         2531 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa agcatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa ccagctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ctattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaatta
//
LOCUS       DAZFRJ010000154        10261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000154 DAZFRJ010000000
VERSION     DAZFRJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.156"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1192
                     /gene="ybaT"
                     /locus_tag="ACHKUN_003587"
     CDS             <1..1192
                     /gene="ybaT"
                     /locus_tag="ACHKUN_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6661782.1"
                     /translation="LGQAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGII
                     DFFRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYAL
                     GIIAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGA
                     FFSCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDV
                     SALELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSE
                     RELPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAI
                     RLRHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKR
                     NKTV"
     gene            1223..1320
                     /locus_tag="ACHKUN_003588"
                     /pseudo
     CDS             1223..1320
                     /locus_tag="ACHKUN_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1317..1724
                     /gene="cueR"
                     /locus_tag="ACHKUN_003589"
     CDS             1317..1724
                     /gene="cueR"
                     /locus_tag="ACHKUN_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO6661783.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1725..2183)
                     /gene="ybbJ"
                     /locus_tag="ACHKUN_003590"
     CDS             complement(1725..2183)
                     /gene="ybbJ"
                     /locus_tag="ACHKUN_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO6661784.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2180..3097)
                     /gene="qmcA"
                     /locus_tag="ACHKUN_003591"
     CDS             complement(2180..3097)
                     /gene="qmcA"
                     /locus_tag="ACHKUN_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6661785.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3243..3920
                     /gene="fetA"
                     /locus_tag="ACHKUN_003592"
     CDS             3243..3920
                     /gene="fetA"
                     /locus_tag="ACHKUN_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO6661786.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3907..4686
                     /gene="fetB"
                     /locus_tag="ACHKUN_003593"
     CDS             3907..4686
                     /gene="fetB"
                     /locus_tag="ACHKUN_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO6661787.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4749..5603)
                     /gene="cnoX"
                     /locus_tag="ACHKUN_003594"
     CDS             complement(4749..5603)
                     /gene="cnoX"
                     /locus_tag="ACHKUN_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO6661788.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5664..6473)
                     /gene="ybbO"
                     /locus_tag="ACHKUN_003595"
     CDS             complement(5664..6473)
                     /gene="ybbO"
                     /locus_tag="ACHKUN_003595"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6661789.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6463..7086)
                     /gene="tesA"
                     /locus_tag="ACHKUN_003596"
     CDS             complement(6463..7086)
                     /gene="tesA"
                     /locus_tag="ACHKUN_003596"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO6661790.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7057..7743
                     /gene="ybbA"
                     /locus_tag="ACHKUN_003597"
     CDS             7057..7743
                     /gene="ybbA"
                     /locus_tag="ACHKUN_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO6661791.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7740..10154
                     /gene="ybbP"
                     /locus_tag="ACHKUN_003598"
     CDS             7740..10154
                     /gene="ybbP"
                     /locus_tag="ACHKUN_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO6661792.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2248 a         2640 c         2865 g         2508 t
ORIGIN      
        1 gctggggcag gctgcattgc taatggaagc ctcgacctgg gtcgcctttg cttttggcgg
       61 tattgtggcg atgttttccg gttatgccta tgcgcgtctg ggggcgagct atcccagcaa
      121 tggcggcatt atcgacttct ttcgtcgcgg attaggcaac ggcgtctttt cgctggcgct
      181 ctcgttactg tacctgttga cgctggcggt gagcatcgcc atggtcgccc gtgcttttgg
      241 cgcttatgcc gtgcagtttt tgcatgaagg cagccaggag gagcacctta ttttgctcta
      301 cgcgttgggg atcattgcgg tgatgacgct tttcaactcc ttaagcaacc atgcggtagg
      361 gcggctggaa gtgatcctcg tcggcattaa aatgatgatc ctgttattgc tgattattgc
      421 cggtgtctgg tcgctgcaac cggcgcatat ttccgtctct gcgcccccca gctccggtgc
      481 gttcttctcc tgtatcggga taaccttcct tgcctatgcg ggctttggca tgatggcgaa
      541 cgcggcggat aaagtgaaag atccgcaggt cattatgccg cgggcgtttc tggtggcgat
      601 tggcgttacc acgttgcttt atatctcgct ggcactggtt ttgcttagcg atgtatcggc
      661 attagagtta gaaaaatatg ccgataccgc cgtagcgcag gctgcttctc cgctgctcgg
      721 acatgtgggt tatgtgatcg tcgtcatcgg cgctttactg gcgacggctt cagccattaa
      781 cgcgaacctg ttcgccgtgt ttaacatcat ggacaacatg ggcagcgaac gcgaactgcc
      841 gaagctaatg aataaatccc tgtggcggca gagtacctgg ggcaacatca ttgtcgtggt
      901 gttgattatg ctgatgacgg cggcactaaa tttaggctca ctcgccagcg ttgccagcgc
      961 cacctttttg atttgctatc tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat
     1021 tcacgcctcg ttgccgattc ttatcgttgg tacgttggtg atgttgttgg tgatcgttgg
     1081 ctttatctac agtctgtggt cccagggtag ccgtgcgttg atatggatta ttggctcact
     1141 cttactcagc cttattgtgg caatggtcat gaagcgcaat aaaaccgtat aacatctctc
     1201 tgtgcgcagt acttcctgta ttattgtggt ggcggtcgat attcgcactg gcaaaaaaac
     1261 gtgcttgaat atctgttgaa accctttaac aaagcacagg aggcgttgcg cgaacgatga
     1321 acatcagcga tgtagcaaaa attaccggcc tgaccagcaa agccattcgc ttctatgaag
     1381 agaaggggct ggtgacgccg ccgatgcgca gcgaaaacgg ttatcgcacc tacacgcagc
     1441 agcatctcaa cgaactgacc ttactgcgcc aggcacggca ggtgggcttt aacctggaag
     1501 agagcggcga gctggtgaat ctgtttaacg acccgcagcg gcacagcgcc gacgtcaaac
     1561 ggcgcacgct ggagaaggtg gcggagatcg aacgacacat tgaggagctg caatccatgc
     1621 gcgaccagct gctggcactg gcgaatgcct gccctggcga tgacagcgcc gactgcccga
     1681 ttatcgaaaa tctctccggc tgctgtcatc atcgggcagg gtgattaaga cgagacggct
     1741 cggatatgta gggttatccc ttctatcgca atgacttcaa catgcgtacc tgcaccgaga
     1801 tcctcgctgg cgctgacagg ccatgaactg tcaccgacgc gcatatgacc gcgcccgttg
     1861 accagcggag attccagcac aaaacgtcgg ccaatcagct gctgcccgcg ctggtttaaa
     1921 tgactatcgc tgtgcttttg ttcgcgcacc cgccgcgaca accatttcca ccacagccag
     1981 gcggcgagca gcgtcaggat ggcaaacatt accccttgcc actcccaacc cagcggcacc
     2041 agccagacca ccaggccggt aatcaccgct gccacgccgc tccacaacaa ataaccattt
     2101 ccgcccagca tctcggctgc cagcagcaaa ccgccgagac tgagccagaa aatatgtgga
     2161 tgaacgacca ttaactccat catggctgag tccgtttgtt ggcgctgtct ttcaccagct
     2221 cggcaatccc ggcaatcgac cccatcaggc tgctggcctc taatggcatc atcactactt
     2281 tgctgttact ggaggaaccg atctgctgta acgcttcggt gtatttctgc gctacgaagt
     2341 agttcaccgc ctgaatatca ccggaggcga tagcttcaga caccattttg gtggcgcggg
     2401 cttctgcttc ggcggaacgt tcacgcgctt cagcctgtaa aaacgccgac tgacgttcgc
     2461 cttccgcttt caggatttgc gactgttttt caccttcggc tttgaggatt tccgcctgac
     2521 ggatcccttc cgcttcaaga atgtaagcgc gtttggtacg ttccgctttc atctgcgcgt
     2581 tcattgaaga gataagctct gccggtgggc gcacgtcgcg aatttcaata cgggtgactt
     2641 taatccccca cgggttggtg gcttcatcga caatacgcag caggcgtgag ttgatgctgt
     2701 cgcgctgaga gagcatttcg tcaagttcca ttgaacccaa cacggtacgg atgttagtca
     2761 tggtcaggtt gatgatcgcc agctccagat tgctgacttc ataagccgcg cgcggcgcgt
     2821 caatcacctg aataaaacag actgcgtcga tggtaacgtt ggcgttatct ttcgagataa
     2881 cttcctggga agggatatcg agcacttgct ccatcatatt gatcttgcga ccaatgcgat
     2941 ccataaacgg caccaccaga ctgagccccg gctgtaacgt tttggtatag cgaccaaaac
     3001 gttctaccgt ccattgatag ccctgcggca cgattttgac gcccgcgccg acaatgacca
     3061 gcgcgacaaa aatgagaatc gggataaaga taagcatcgg aaaaacctcc tgttgtaccg
     3121 tccataatca gcaaaattgc tgcttgatta aacaaattat acctgattac tgaaagagag
     3181 ttccccctta ttcctgcgaa ggataaactg tttttagtaa aaatcagaaa aagggaacag
     3241 tgatgcagga aaatagtcct ttacttcagc tacaaaacgt aggatatctg gcgggtgatg
     3301 cgaagattct taataacatc aatttttcgc tgcgtgctgg cgaatttaag ttaattaccg
     3361 gtccttccgg ttgtggcaaa agtacgctgc taaaaatagt tgcttcattg atcagcccaa
     3421 ccaacggaac gttactgttt gaaggtgagg atgtcagcac actaaagcca gaaatctacc
     3481 gccagcaagt ctcttactgc gcccagacgc cgacgctgtt tggcgatacg gtatacgata
     3541 atctgatctt tccctggcag atccgtaacc agcagcctga cccggccatt tttctcgatt
     3601 ttctcgaacg cttcgctttg ccggacagca ttttgacgaa aaatatcgcc gagctatctg
     3661 gtggtgaaaa acaacgcatc tcactgattc gtaacctgca atttatgccg aaggttttat
     3721 tgctggatga aatcaccagt gcgctggatg aaagtaataa acataacgtt aatgagatga
     3781 tccatcgtta tgtgcgcgag caaaatattg ccgtgctgtg ggtgacacac gataaagacg
     3841 aaattaatca tgcggataaa gtgattacac tgcaaccgca tgccggagaa atgcaggaag
     3901 cacgctatga actcgcataa tattactaac gaatcattgg cactggcatt aatgctggtg
     3961 gtggtggcaa tcttaattag ccataaagaa aaactggcgc tggagaaaga tattttctgg
     4021 agcgtcgggc gagcgataat tcagctgatt attgtcggct atgtgctgaa gtatattttc
     4081 agcgtggatg atgccagcct gacattattg atggtgttat ttatctgctt taatgcggcg
     4141 tggaacgcgc aaaaacgcag taaatatatt gctaaagctt ttatctcatc gtttattgcc
     4201 attacggtcg gggcgggaat taccctggcg gtgctgattc tctctgggtc gattgaattt
     4261 atcccgatgc aggtgatccc tatcgccggg atgattgccg gtaacgccat ggtagcggtg
     4321 gggttgtgtt acaacaattt agggcaacgg gtcattagcg aacagcaaca gattcaggag
     4381 aaactgagtc ttggtgcgac gccgaagcag gcttcagcga tattgattcg cgacagtatt
     4441 cgcgcggctt taattccgac ggtcgattca gcaaaaacgg ttggcttagt gagtttacct
     4501 ggaatgatgt ccgggctgat atttgccggg attgatccgg tgaaggcgat taaatatcag
     4561 attatggtga cctttatgct gctctcaacc gccagcttgt cgaccattat tgcctgctat
     4621 ttaacctatc gtaagtttta taattcgcgc caccagttgg tggtgacgca attgaagaag
     4681 aaatgatgcg atgccggatg cgcagcatcg catccggcgt tgtggttcat gtgccgatca
     4741 acggaatatc aatacaacaa tgcatacagc tggcggcgat acttcgacgc cagtgcatca
     4801 cccgtaccca gcgcagcgag gatctcctga aacgttttac gcgtctgacc gtctgcggcg
     4861 gtgagatctt tacgcagatg cccgaacagc aactccagcg cctcttcatt gcgcccaacc
     4921 tggtgcagtt gcagagccag ttgcgtcgcc agtgcggcat cttctggatt ctccgccacc
     4981 tgctgttgca actgttgaat ttccggcgta tcagccgcct gcttcagcag ttcgatttgc
     5041 gccaccagcc cctggtagcg ggtgtcctga tcctgcaacg gaatggtttt cagcaccgtt
     5101 tccgcatctt cagaacggtt cagcgcaatc agcgtttctg ccagcagcag gccgatctcc
     5161 ccgttctgat tcgacaactg ccaggcgtct ttcagcaatg gcagggcatc ggtgtaattg
     5221 ccttcctgca tcagttgcat cgcctgctgc gctttcagct cttcttcgcg cggcagcact
     5281 ttatccagca gggcgcggat cgcctcttcc ggttgcggcc cctggaagcc atctaccggt
     5341 tgcccgttct ggaacagata cacggtcgga atcgcgcgca gaccaaactg ggcggcaatc
     5401 atctgctccg cgtcgcagtc cagcttcgcc agaataaatt gcccgttgta ctgcgccgcg
     5461 aggctttcca ggatcggggt taactgcaaa cagtgctggc tacgttcaga ccaaaaatag
     5521 aacagcaccg gagtggtcat cgactgttca agaacctgtt gcaggttaga ttcgttaatg
     5581 ttgacaatat tttctacgga catggagtcg ctctctgttg tcgatatttt ctttgacatg
     5641 ggggcttaag cgcgcgcttc aactcacccc tgcaatattt tgtccatcac gcgccccggc
     5701 agcaggcgct tgagcaccat tactgcccag gtcaccagcg ttaccggata gcgcatcttc
     5761 ggcttctcgc taataaaagc atggcgtact ttgtccacca ccgcttccgg tcccaacgta
     5821 aagcgggcgg cgatgccggg attttcgact ggtttatcac tttgcgtctg gttgacgttg
     5881 tcggtgaagc gagtacgaat gggaccgggt tcgatcaggc tgactttaat tccgctgtgg
     5941 cgcagctcca tgcgcagtgc gtctgaccac gcctccagcg catatttact ggccgcgtaa
     6001 gcgccacgac ccggcgtgga gattaatccc atcaccgatg atgtcatcac aatacgccct
     6061 tcaccgtgcg gtaacatcgc gggtaacagg cgcatggtga gctggtgtgc gccgaaaaag
     6121 ttggcggaaa actgctgttc catctgcgca cggctgatgg tggaaagggg gccatacatg
     6181 ccgaatccgg cattgttaaa gatcccatac agacaattat cggtcagggc gatcacctcg
     6241 tctgctgcgc gatcaacact ttctggtgaa tccagatcga tcaacacgcc ggtaaatccc
     6301 atgttgttca tacgctcaac atcatccggt tttcggcaac ctgccagcac atgaaaaccc
     6361 tggcgtttta attcgagcgc gctttccagg ccaattccac tggaacatcc ggtaattaag
     6421 accgattttt gcataacttt acctgtcagg atctccgttg ctttatgagt catgatttac
     6481 taaaggctgc aactgcttcg ccatccagtc ggcaataaac ggctgggcgt cgcggttggg
     6541 atgaataccg tcatcctgca tccattgtgg cttgaggtag acctcttcca taaaaaaggg
     6601 cagcagcgga acatcaaact ctttggcgag tttggggtaa atggcgctaa aggcttcatt
     6661 ataacggcga ccatagtttg caggcagacg tatttgcatt aacaatggtt cagcgttggc
     6721 ggctttgaca tcctgcaaaa tctggcgcag cgtttgctcg gtttgctgtg gctgaaaacc
     6781 acgcaaaccg tcattgccgc ccagttcaac cagcacccag cgcggctgat gctgtttcag
     6841 cagagccgga aggcgcgcca gtccttgttg cgaggtgtcg ccgctgatgc tggcattaac
     6901 taccgacgtt ttactctgcc acttatcatt caacaaggca ggccaggccg cgctggcaga
     6961 cattcgatac ccggcgctca ggctatcacc cagaatcaat aacgtgtccg ctgcggcggc
     7021 acggaaggtt aacaggacca ggaacaggaa gggcaaatgc cagcggaaaa cattgttgaa
     7081 gttcatcatc ttaagaagtc cgtcggtcag ggggagcatg aactctccat cctcaccgga
     7141 gttgagctgg ttgtcaaacg tggcgagacc atcgcactgg tgggcgaatc gggatcgggt
     7201 aagtcaacct tgctggcgat cctcgccggg cttgatgacg gcagcagtgg cgaagtgagt
     7261 ctggtgggac aaccgctaca taatatggac gaagaagcgc gggcaaagtt gcgcgcgaag
     7321 cacgtcggct ttgtttttca gtcatttatg ttaattccta cccttaacgc gctggaaaat
     7381 gtcgagcttc cggctctgct gcgcggtgag agtagcgcgg aaagtcgtaa cggggcgaaa
     7441 gcgttgctcg aacagttagg gctgggtaaa cgtctggatc atcttccggc acagctttcc
     7501 ggcggtgaac agcaacgagt ggcgctggca cgagccttta atggtcgacc tgatgtgctg
     7561 tttgccgacg aacccaccgg caaccttgac cgccagacgg gcgataaaat tgccgacctg
     7621 ctgttttccc tcaaccgtga acatggcacc acgttgatta tggtgactca cgacctgcaa
     7681 ctggcggcac gctgcgaccg ctgcttacgg ctggtgaacg ggcagttgca ggaggaagca
     7741 tgattgcacg ttggttctgg cgcgaatggc gctcgccgtc gctattaatt gtctggctgg
     7801 cgctaagcct ggcggtggcc tgcgtgctgg cgctgggcaa tatcagcgat cgcatggaga
     7861 agggcttaag ccagcaaagc cgtgagttta tggcgggcga tcgggcgttg cgcagttcgc
     7921 gcgaagtgcc gcaagcatgg ctggaggaag cgcaaaagcg cggcctgaaa gtcggcaagc
     7981 agctgacttt cgccaccatg acatttgcag gcgacacacc gcagctggcg aacgtcaaag
     8041 cggtggatga tatctacccg atgtatggcg atctgcaaac taatccccct ggcctgaaac
     8101 cgcaggcggg cagcgtattg ctggccccac gcctgatggc actgcttaac ctgaaaacgg
     8161 gcgacaccat tgacgtgggc gatgccacct tgcggattgc cggagaagtg attcaggaac
     8221 cggattccgg ttttaacccc ttccagatgg ctccgcgtct gatgatgaat ctggcggatg
     8281 tcgataaaac cggagccgtg cagccgggga gtcgggtcac ctggcgttat aaattcggcg
     8341 gcaacgagaa ccagctcgac ggctatgaga aatggttgtt acctcagctt aaacccgaac
     8401 aacgctggta cggtctggaa caggacgaag gcgcgctggg gcgatcgatg gaacgctcgc
     8461 aacagttcct gctgctttcg gcgcttctga ccttgctgct ggcagtggca gcggtggcgg
     8521 tagcgatgaa tcattactgt cgcagtcgct acgatctggt ggcgatcctc aaaacgctgg
     8581 gggcagggcg agcgcaactg cgtaagctaa tcgtcggtca gtggttgatg gtgctggcgc
     8641 tttcagccgt taccggtggg gcgataggcc tgttgttcga aaacgtgttg atggtgctgc
     8701 tcaagcccgt tctgcctgct gcactaccgc cagccagcct ctggccgtgg ctgtgggcgc
     8761 tgggtaccat gacggtcatc tcgctgctgg tggggctacg accgtaccgc ctgttgctgg
     8821 caacgcagcc tttacgcgta ttacgtaatg atgtggtagc gaacgtctgg ccgctgaagt
     8881 tttatctgcc gattgtcagt gtggtggttg tgctgctgct cgccggatta atgggtggca
     8941 gcatgctgct ttgggcggtg ctggcgggcg cggtagtact ggctttgctg tgcggtgtgc
     9001 tgggctggat gctgctgaat gtacttcgcc gcatgacgct gaaatcgcta cctctgcgcc
     9061 tggcggttag ccgcctgtta cgtcagccgt ggtcaacatt aagccagctt tcggcatttt
     9121 cgctctcctt tatgctgctg gcgctgttgc tggtattgcg tggcgatctg cttgaccgct
     9181 ggcagcagca gctacctcca gaaagcccga actacttttt aattaacatt gccacagaac
     9241 aggtagaacc gctaaaagcg ttcctcgcgg aacatcagat agtcccggaa tcattttatc
     9301 cggtagtgcg ggcgcggctg acggcgatta acgataagcc gacagaaggt aatgaagatg
     9361 aggcgcttaa ccgcgaactc aatcttacct ggcaaaatac gcgacccgat cataatccga
     9421 ttgtcgccgg taactggccg ccaaaagccg atgaagtgtc gatggaagag gggctggcga
     9481 aacgcttaaa cgttgccctc ggtgataccg tgacttttat gggcgatacc caggagttcc
     9541 gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag tctgcggcct aatttctatt
     9601 ttattttccc tgaaggggca ttagacgggc aaccgcagag ctggcttacc agtttccgct
     9661 gggagaatgg caacggcatg ttgacacaac tcaaccgcca gttcccgacc attagcctgt
     9721 tagatattgg cgcgatttta aaacaggtcg gtcaggtgct ggagcaggta agtcgggcgc
     9781 tggaggtgat ggtggtgctg gtcaccgcct gcggtatgtt gctgttgctg gcacaggtgc
     9841 aggtgggaat gcgccagcgt catcaggagc tggtggtatg gcgcacactc ggtgcgggga
     9901 aaaaactgct gcgcacgaca ttgtggtgtg agttcgccat gcttgggttt gtttccggcc
     9961 tggtggccgc aattggtgcg gaaacggcac tggcagtgtt gcaggcgaaa gtgtttgatt
    10021 tcccgtggga gccagactgg cgattgtgga ttgttctgcc gtgcagcggt gcgctgctgc
    10081 tgtcgctttt cggcggctgg ctgggtgcgc gactggttaa gggtaaggcg ctgttcaggc
    10141 agtttgcggg gtgatgagag taaataaggg gcgttctggt tgaatggaac gccttgttta
    10201 aattaattct acaatatgtt tcattgatga tgttattgaa ttggtgattt cctatctttc
    10261 t
//
LOCUS       DAZFRJ010000155        10200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000155 DAZFRJ010000000
VERSION     DAZFRJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.157"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            97..1491
                     /gene="galP"
                     /locus_tag="ACHKUN_003599"
     CDS             97..1491
                     /gene="galP"
                     /locus_tag="ACHKUN_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO6661793.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1568..2065
                     /locus_tag="ACHKUN_003600"
     CDS             1568..2065
                     /locus_tag="ACHKUN_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO6661794.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2160..2867
                     /gene="endA"
                     /locus_tag="ACHKUN_003601"
     CDS             2160..2867
                     /gene="endA"
                     /locus_tag="ACHKUN_003601"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO6661795.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2947..3678
                     /gene="rsmE"
                     /locus_tag="ACHKUN_003602"
     CDS             2947..3678
                     /gene="rsmE"
                     /locus_tag="ACHKUN_003602"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO6661796.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3691..4641
                     /gene="gshB"
                     /locus_tag="ACHKUN_003603"
     CDS             3691..4641
                     /gene="gshB"
                     /locus_tag="ACHKUN_003603"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO6661797.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4750..5313
                     /locus_tag="ACHKUN_003604"
     CDS             4750..5313
                     /locus_tag="ACHKUN_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO6661798.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5313..5729
                     /gene="ruvX"
                     /locus_tag="ACHKUN_003605"
     CDS             5313..5729
                     /gene="ruvX"
                     /locus_tag="ACHKUN_003605"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO6661799.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5905..6885)
                     /locus_tag="ACHKUN_003606"
     CDS             complement(5905..6885)
                     /locus_tag="ACHKUN_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO6661800.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6903..7607
                     /gene="yggS"
                     /locus_tag="ACHKUN_003607"
     CDS             6903..7607
                     /gene="yggS"
                     /locus_tag="ACHKUN_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO6661801.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7625..8191
                     /gene="yggT"
                     /locus_tag="ACHKUN_003608"
     CDS             7625..8191
                     /gene="yggT"
                     /locus_tag="ACHKUN_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO6661802.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8188..8478
                     /gene="yggU"
                     /locus_tag="ACHKUN_003609"
     CDS             8188..8478
                     /gene="yggU"
                     /locus_tag="ACHKUN_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO6661803.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8486..9079
                     /locus_tag="ACHKUN_003610"
     CDS             8486..9079
                     /locus_tag="ACHKUN_003610"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO6661804.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9072..>10200
                     /gene="hemW"
                     /locus_tag="ACHKUN_003611"
     CDS             9072..>10200
                     /gene="hemW"
                     /locus_tag="ACHKUN_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO6661805.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFL"
BASE COUNT         2386 a         2703 c         2707 g         2404 t
ORIGIN      
        1 gtaagcgatt acactgatgt gatttgcttc acatcttttt acgtcgtact cacctatctt
       61 aattcacaat aaaaaataac catattggag ggcatcatgc ctgacgctaa aaaacagggg
      121 cggtcaaaca aggcaatgac ttttttcgtc tgcttccttg ccgctctggc gggattactc
      181 tttggcctgg atatcggtgt aattgctggc gcactgccgt ttattgcaga tgaattccag
      241 attacttcgc acacgcaaga atgggtcgta agctccatga tgttcggtgc ggcagtcggt
      301 gcggtgggca gcggctggct ctcctttaaa ctcgggcgca aaaagagcct gatgatcggc
      361 gcaattttgt ttgttgccgg ttcgctgttc tctgcggctg cgccaaacgt tgaagtactg
      421 attctttccc gcgttctgct ggggctggcg gtgggtgtgg cctcttatac cgcaccactg
      481 tacctgtcag aaattgcgcc ggaaaaaatt cgcggcagta tgatctcgat gtatcagttg
      541 atgatcacta tcgggatcct cggtgcttat ctttctgata ccgccttcag ctacaccggt
      601 gcatggcgct ggatgctggg tgtgattatc atcccggcaa ttttgctgct gattggtgtc
      661 ttcttcctgc cagacagccc acgttggttt gccgccaagc gccgttttgt tgatgccgaa
      721 cgcgtgctgc tacgcctgcg tgacaccagc gcggaagcga aacgcgaact ggatgaaatc
      781 cgtgaaagtt tgcaggttaa acagagtggc tgggcgctgt ttaaagagaa cagcaacttc
      841 cgccgcgcgg tgttccttgg cgtactgttg caggtaatgc agcaattcac cgggatgaac
      901 gtcatcatgt attacgcgcc gaaaatcttc gaactggcgg gttataccaa cactaccgag
      961 caaatgtggg ggaccgtgat tgtcggcctg accaacgtac ttgccacctt tatcgcaatc
     1021 ggccttgttg accgctgggg acgtaaacca acgctaacgc tgggcttcct ggtgatggct
     1081 gctggcatgg gcgtactcgg tacaatgatg catatcggta ttcactctcc gtcagcgcag
     1141 tatttcgcca tcgccatgct gctgatgttt attgtcggtt ttgccatgag tgccggtccg
     1201 ctgatttggg tactgtgctc cgaaattcag ccgctgaaag gccgcgattt tggcatcacc
     1261 tgctccaccg ccaccaactg gattgccaac atgatcgttg gcgcaacgtt cctgaccatg
     1321 ctcaacacgc tgggtaacgc caacaccttc tgggtgtatg cggctctgaa cgtactgttt
     1381 atcctgctga cattgtggct ggtaccggaa accaaacacg tttcgctgga acatattgaa
     1441 cgtaatctga tgaaaggtcg taaactgcgc gaaatcggcg ctcacgatta atctcctcag
     1501 gcttcctccc atcgcggagg aagccacctc ttgcagtcat cttttcttcg ctctatcctc
     1561 tgccgctatg aaaacatccc gtctccctat cgccatccag caggccgtta tgcgccgcct
     1621 gcgggaaaaa ctcgcccagg ccaacctgaa gctagggcgt aactacccgg agccaaaact
     1681 ctcttacacc cagcgcggaa cctccgccgg aacagcctgg ctggaaagct atgaaatccg
     1741 cctcaatccc gttttgctgt tggaaaacag tgaagctttt attgaagaag tggtaccgca
     1801 cgaactggca catttgctgg tatggaaaca tttcggccgc gtagcgccac atggcaaaga
     1861 gtggaagtgg atgatggaaa gcgtgctggg cgttcccgcc cgtcgtacgc atcagttcga
     1921 actgcaatcc gtgcgtcgca acaccttccc ctaccgctgc aagtgccagg agcatcagct
     1981 taccgtacgc cgccataatc gcgtagttcg tggcgaggcc gtctatcgct gtgttcactg
     2041 cggtgaacag ctggttgcga aataaccatc tgaactatca ggaactttcc tgatctggct
     2101 gattgcatac caaaacaact ttcgctacgt tgctggctcg ttttaacacg gagtaagtga
     2161 tgtaccgtta tttgtctatt gctgcggtgg tactgagcgc agcattttcc ggcccggcgt
     2221 tggccgaagg tatcaatagt ttttctcagg cgaaagccgc ggcggtaaaa gtccacgctg
     2281 acgcgcccgg tacgttttat tgcggatgta aaattaactg gcagggcaaa aaaggcgttg
     2341 ttgatctgca atcgtgcggc tatcaggtgc gcaaaaatga aaaccgagcc agccgcgtag
     2401 agtgggaaca cgtcgttccc gcctggcagt tcggtcacca gcgccagtgc tggcaggacg
     2461 gtggacgtaa aaactgcgct aaagatccgg tctatcgcaa gatggaaagc gatatgcata
     2521 acctgcaacc gtcagtcggc gaggtgaatg gcgatcgcgg caactttatg tacagccagt
     2581 ggaatggcgg cgaaggccag tacggtcaat gcgccatgaa ggtcgatttc aaagaaaaag
     2641 ctgccgaacc accagcgcgt gcacgcggtg ccattgcacg cacctacttc tatatgcgcg
     2701 accaatacaa cctgacactc tctcgccagc aaacgcagct gttcaacgca tggaacaaga
     2761 tgtatccggt taccgactgg gagtgcgagc gcgatgaacg catcgcgaag gtgcaaggca
     2821 atcataatcc gtatgtgcaa cgcgcttgcc aggcgcgaaa gagctaacct acactagcgg
     2881 gactcttttt gttaacccct accccacgcg tacaaccgcg tggggagacg acgcggattt
     2941 ttaactatgc gtatcccccg catttatcat cctgaaccac tgaccagcca ttctcacatc
     3001 gcgctttgcg aagatgccgc caaccatatc gggcgcgtac tgcgcatggg gccggggcag
     3061 gcgttgcaat tgtttgacgg tagcaaccag gtctttgacg ccgaaattac cagcgccagc
     3121 aaaaaaagcg tggaagtgaa ggtgctggaa ggcaagatcg acgatcgcga atctccgctg
     3181 catattcacc tcggtcaggt gatgtcgcgt ggtgaaaaaa tggaatttac gatccagaaa
     3241 tcgatcgaac tcggtgtaag cctcattacg ccactttttt ctgagcgctg cggcgttaaa
     3301 ctggatagtg aacgtctgaa caagaagctc cagcagtggc agaagattgc aattgctgcc
     3361 tgtgagcagt gtggtcgtaa ccgggtgccg gaaatccgcc cagcgatgga tctggaagcc
     3421 tggtgtgcag agcaggatga aggactgaaa ctgaatcttc acccgcgcgc cagtaacagc
     3481 atcaatacgt tgccgttacc ggttgaacgc gtccgcctgc tgattggccc ggaaggcggt
     3541 ttatcggcag atgaaattgc catgactgcc cgctatcaat ttactgatat cctgttggga
     3601 cctcgcgttt tgcgtacaga gacaactgcg ctcaccgcca ttaccgcgct acaagtacga
     3661 tttggcgatt tgggctaacg gagaagaata atgatcaagc tcggcatcgt gatggacccc
     3721 atcgcaaaca tcaacatcaa gaaagattcc agtttcgcta tgttgctgga agcacagcgt
     3781 cgtggttacg aacttcacta tatggagatg ggcgatctgt atctgatcaa tggcgaagcc
     3841 cgcgcccata cccgcacgct gaacgtgaag cagaactacg aagagtggtt ttcgttcgtc
     3901 ggtgaacagg atctgccgct ggccgatctc gatgtgatcc tgatgcgtaa agacccgccg
     3961 tttgataccg agtttatcta cgcgacctat attctggaac gtgccgaaga gaaagggacg
     4021 ctgatcgtta acaagccgca gagcctgcgc gactgtaacg agaaactgtt taccgcctgg
     4081 ttctctgact taacgccaga aacgctggtt acgcgcaata aagcgcagct aaaagcgttc
     4141 tgggagaaac acagcgacat cattcttaag ccgctggacg gtatgggcgg cgcgtcgatt
     4201 ttccgcgtga aagaaggcga tccaaacctc ggcgtgattg ccgaaaccct gactgagcat
     4261 ggcactcgct actgcatggc gcaaaattac ctgccagcca ttaaagatgg cgacaaacgc
     4321 gtgctggtgg tggatggcga gccggtaccg tactgcctgg cgcgtattcc gcaggggggc
     4381 gaaacccgtg gcaatctggc tgccggtggt cgcggtgaac ctcgtccgct gacggaaagt
     4441 gactggaaaa tcgcccgtca gatcgggccg acgttgaaag aaaaagggct gatttttgtt
     4501 ggtctggata tcatcggcga ccgtctgact gaaattaacg tcaccagccc aacctgtatt
     4561 cgtgagattg aagcagagtt tccggtgtcg atcaccggaa tgttaatgga tgccatcgaa
     4621 gcacgtttac agcagcagta acccacctta gcgagaagga tctcgttgag actctgagtg
     4681 acagcgccct tctttccacg catactgggc gctgttgctt ttttgaacca ggaaacagaa
     4741 cctctgacaa tgaatttaca gcatcacttt cttattgcca tgcctgctct ccaggatccg
     4801 attttccgtc gttccgtggt ctacatttgc gaacataata ccaatggtgc aatggggatc
     4861 atcgtcaaca agccgctgga aaatctcaaa attgaaggga ttctggaaaa gctgaagatc
     4921 acgccggagc cgcgtgatga atcaatccgt ctggataaac cggttatgct cggcggtccg
     4981 ctggctgaag atcgcgggtt tattttgcat actccgccct ccaattttgc ttccagcatt
     5041 cgcatttcag acaacacagt gatgaccact tcccgcgatg tgctggaaac gctcggcacc
     5101 gataaacaac cgtctgacgt attggtggct ctgggttatg cctcctggga aaaaggtcaa
     5161 ctggaacaag aaattctcga taacgcgtgg ctaacggccc cggcggatct gaatattctg
     5221 ttcaaaacgc cgattgccga ccgctggcgc gaggcggcaa aactgattgg tgtggatatt
     5281 ctcaccatgc ctggtgtggc agggcacgcc tgatgagtgg aaccttactc gccttcgact
     5341 tcggcaccaa aagcattggc gtagcggtcg gccaacgcat taccggcact gctcgccctt
     5401 tgcctgcaat taaagcacag gacggtacgc cggactggaa cctcatcgaa cgtttactga
     5461 aagagtggca gccggacgaa atcatcgtcg gtttgccgct gaatatggac ggcaccgagc
     5521 aaccattgac tgccagagcg cgtaaatttg ccaaccgtat tcatggtcgt ttcggtgttg
     5581 aagtaaagct ccatgacgaa cgtcttagca ccgtggaagc ccgttccggt ctgtttgaac
     5641 agggcggcta tcgggcgctc aacaaaggca aagtcgactc tgcctctgcg gttattattc
     5701 tcgaaagcta tttcgagcag ggatattaag gcgatctaaa agcctggcgg atagtcgtta
     5761 ccgccatctt gccggatgcg gcgtgaacgc cttatccagc acacatctgg cagcggctgt
     5821 aggtctgata agacgcgtta gcgtcgcatc agacatttat tgcctttata ggccctataa
     5881 acttgcgcat cgggcatggc aacgtcacaa acgcccttcc cccacccgct gctgataact
     5941 ctgctgaaac gttaacatcc ccacctgctg cccggtttga ataacatgcg gtaactggtg
     6001 ggttttccct tcgcgaatca aattccccac cgcgggagtg ttaatcaata attcaaacag
     6061 cgccacgcgt ccttcctgtt tatccacttc cagcttttgt gacaacacgg cccgtaaact
     6121 acctgccagt tgattacgta cgggatcttt ttcctgcgcc ggaaatgaat ccaccagtcg
     6181 ctcaactgcc tgcgcggcac cacgcgtatg taatgttgcc agcaccaaat gcccggtttc
     6241 tgccgccgtc agtgccagac ggattgtctc gctgtcacgc agctctccga gcaaaatcac
     6301 atcaggatct tcccgcaatg cggcccgcaa tcccgatgcg aacgtcatac agtgcaaacc
     6361 aatttcccgc tgctggatca aacatcgctg gctggcatag atatattcaa caggatcttc
     6421 cagcgtcaga atatgcgcat cggcatgttg atagagatag ccaaccatcg ccgctagcgt
     6481 ggtagatttg ccgctccccg tcgcccccgt caccagaatc aggccattct cgctcttgag
     6541 taattccggc aataccgttg gtgcgccaag ctgttcgagc tgtggacagt gcgaaggtaa
     6601 caaccgtaat gccagagaaa taccttgccg ttgcgcgaac gcactgccac gcaaccgctg
     6661 gttttccgcc agcgacacgg caaaatccag ctggccattt tccagcaata ttgcccgctg
     6721 atcgtcatcc agccactccc gcagtagctc ttcgacgtcc ggcgcatcaa acggcgcagc
     6781 ttccattagc ccgcgaatgc gccatcgtgc ggaccaggcg ctgcacaggt gtagatccga
     6841 gacgttatgc tttacactaa gggccacaat ttcttccata ttcatactaa gatcctcgga
     6901 aaatgaacga tattgcgcat aacctggcac aggtccggga caaaatctca gcggctgcaa
     6961 cgcgttgcgg ccgttctcca gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg
     7021 cgagcgccat cgcagaagcc attgatgccg ggcagcgtca atttggtgaa aactacgttc
     7081 aggaaggggt agataaaatt cgccactttc aggaactggg cgtaacagga ttagaatggc
     7141 attttattgg cccgttgcag tctaataaaa gccgcctggt ggcagagcat ttcgactggt
     7201 gtcataccat cgaccgtttg cgcatcgcta cccgcctcaa cgatcagcgc ccggcagaac
     7261 ttccccctct taacgtactg attcaaatta atattagtga tgaaaacagt aagtccggga
     7321 ttcaactggc agaactggac gagctggcag ctgcggtcgc tgaactaccg cgtttacgtc
     7381 tgcgcgggct gatggcaatc cctgcacctg agtcagaata tgtaaggcag tttgaagttg
     7441 cacgccaaat ggctgtagca tttgccggac tgaaaacgcg ctacccgcat atcgacacgc
     7501 tctctctggg aatgtcggac gatatggaag ccgccattgc ggcaggtagc acgatggttc
     7561 gtatcggcac tgcaattttt ggtgcgcgtg attactctaa aaaataagga attaaaggaa
     7621 cgccatgaat acgttgactt tcctgctttc aacggtcatt gagctgtata ccatggtgct
     7681 gttattacgc atctggatgc agtgggctca ttgtgatttt tacaacccct tctcacagtt
     7741 tgtagtgaag gtaacgcagc caattatcgg gccactgcgc cgcgttattc cggcaatggg
     7801 ccccattgac agcgcctcgc tgctggttgc ctatattctc agttttatca aagccatcgt
     7861 gctgtttaaa gtggtgacct tcctgccaat catctggatt gccggtttac tgattctgct
     7921 gaaaaccatc ggcctgctga ttttctgggt cctgctggtg atggcgatta tgagctgggt
     7981 aagccagggt cgtagcccga ttgaatacgt gctgattcag ctggccgatc cgctgctgcg
     8041 cccgattcgc cgcctgctac cggcaatggg tgggattgat ttctcgccga tgatcctcgt
     8101 tctgctgctg tatgtcatca atatgggtgt cgcagaagta ttacaggcaa ccggaaacat
     8161 gctgctgccg gggctgtgga tggcgttatg agtgccgtaa cagttaatga tgacggtctg
     8221 gttttacggc tctatattca gccgaaagcc agccgtgatt ctattgtcgg tttacatggc
     8281 gacgaagtta aagtcgccat taccgcgccg ccggttgacg gtcaggccaa cagtcatctg
     8341 gtgaagtttc tcggtaagca attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc
     8401 gaacttggcc gccacaaaca aattaaaatc attaatccgc aacaaatccc gccagaaatc
     8461 gcggcgttaa ttaattaggt atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg
     8521 taaagtgcgt gagctggcgt cgctgcttag cgacttcggt cttgatatcg tggcccaaac
     8581 agacctcggc gtagattccg ctgaagaaac cggcctgacc tttatcgaaa acgcgattct
     8641 gaaagcgcgc catgcggcaa aagtgaccgg tttaccggca attgccgacg actctggtct
     8701 ggcggtagat gtgcttggcg gcgcgccggg catttactcc gcgcgttatt ccggtgaaga
     8761 cgcgaccgat ctaaagaatc tgcaaaaact gctggaaaca ctgaaagacg taccggatga
     8821 ccaacgtcag gcgcgtttcc actgcgtgct ggtatatctg cgtcacgcgg aagatccgac
     8881 tccgctggta tgccacggta gctggccggg cgtgattact cgtgaaccag cgggcactgg
     8941 tggctttggt tatgatccaa tcttcttcgt accttccgaa gggaaaaccg ccgccgaact
     9001 gacccacgaa gaaaagagcg ccatttccca ccgtggtcag gcattgaaac tgctgctgga
     9061 cgctttacgt aatggttaaa ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg
     9121 tgcagaaatg cccgtactgc gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg
     9181 acgattacgt tcagcatctg ctttgcgatc tggacaacga tgtggcttac gctcagggcc
     9241 gtgaagtaaa gacaatcttt attggcggtg gtacgccgag cctgctttcc ggcccggcga
     9301 tgcaaacgct gctggacggc gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta
     9361 ctatggaagc gaaccctggt acggtagaag ccgatcgctt tgtcgattat cagcgtgctg
     9421 gtgtgaaccg catctctatt ggtgtgcaga gttttagcga agacaagctg aaacgacttg
     9481 ggcgtattca tggcccgcaa gaagcgaaac gcgcggcaaa gctggcgagc gggctggggc
     9541 tgcgtagctt taacctcgat ttgatgcatg gactaccgga tcaatcactg gaagaggcgc
     9601 ttggcgatct acgccaggcc attgaactga atccgccgca tctttcctgg tatcaactga
     9661 ccatcgaacc taatacgctg tttggttcac gcccgccggt actgccggac gacgacgcgc
     9721 tatgggatat attcgaacag gggcatcagt tattaaccgc agcgggttat cagcaatatg
     9781 aaacttccgc ttacgccaaa tccagttatc agtgccagca caatctcaac tactggcgct
     9841 ttggcgacta catcggtatt ggctgcggcg cgcacggcaa agtgaccttc ccggatgggc
     9901 gcattctgcg taccactaaa acgcgtcatc cgcgtggttt tatgcaagga aggtatctgg
     9961 aaagccagcg tgatgtcgaa gccgcagata agccgtttga gttctttatg aatcgcttcc
    10021 gtctgctgga ggccgcgccg cgcgtggagt ttattgcgta taccgggctt tgcgaagatg
    10081 tgattcgccc acagttagac gaggcgattg cccagggtta tctcaccgaa tgtgcggatt
    10141 actggcagat aacggaacat ggtaaattat tcttaaactc acttcttgaa ttattcctcg
//
LOCUS       DAZFRJ010000156        10136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000156 DAZFRJ010000000
VERSION     DAZFRJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.158"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACHKUN_003612"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACHKUN_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO6661806.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHKUN_003613"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHKUN_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO6661807.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHKUN_003614"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHKUN_003614"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO6661808.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACHKUN_003615"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACHKUN_003615"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO6661809.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHKUN_003616"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHKUN_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO6661810.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACHKUN_003617"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACHKUN_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO6661811.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..7248
                     /gene="hofC"
                     /locus_tag="ACHKUN_003618"
     CDS             6046..7248
                     /gene="hofC"
                     /locus_tag="ACHKUN_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO6661812.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHKUN_003619"
     CDS             complement(7283..8326)
                     /gene="guaC"
                     /locus_tag="ACHKUN_003619"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO6661813.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8482..8526)
                     /locus_tag="ACHKUN_003620"
     CDS             complement(8482..8526)
                     /locus_tag="ACHKUN_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661814.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8551..9171
                     /gene="coaE"
                     /locus_tag="ACHKUN_003621"
     CDS             8551..9171
                     /gene="coaE"
                     /locus_tag="ACHKUN_003621"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO6661815.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9171..9914
                     /gene="zapD"
                     /locus_tag="ACHKUN_003622"
     CDS             9171..9914
                     /gene="zapD"
                     /locus_tag="ACHKUN_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO6661816.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9924..10121
                     /gene="yacG"
                     /locus_tag="ACHKUN_003623"
     CDS             9924..10121
                     /gene="yacG"
                     /locus_tag="ACHKUN_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO6661817.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2489 a         2668 c         2596 g         2383 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagcgggt
       61 gcattcgctg cctcatacca ttattcttga tctgacggaa gtctttttgt aacaattcaa
      121 acttctttga tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca accaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac cgggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggctgagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa atccccacct
      601 gggtttctgc cgccgtattc tttgtggtga ttaacgccat caacctgacc aacgttaaag
      661 tgtttggcga gatggagttc tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga
      721 tcatcttcgg cggctggctg ctattcagtg gcaacggcgg cccgcaggcg accgttagca
      781 acctgtggga tcagggtggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg
      841 cgatgatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtata ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgtgg tgtaccagta aataccattc tggtgtctgc actggtaacg gcgttgtgcg
     1261 tactgattaa ctaccttgcc ccagagtccg ctttcggact gttaatggcg ctggtggtat
     1321 ctgcactggt aatcaactgg gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc cgtacagggt
     1621 gagggcaata atctttacac caacgccccg taaatcgtca gtagtgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgccc tcggcgtctc caccttatcg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaacacctg atactgcgaa
     1801 gtatggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc
     1861 aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg aagcacggca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctgatatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagtagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc acacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt tttagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtagccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgatatta cggcaaaact tttaccggaa aattctcgct ctcatgccac
     3301 ggtgatcacc cgcgagaatg gcgtcttttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 tgccaatcaa tccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattgctg gaaggcacca acacgcagtt gttggatacg cgcaaaacct tacccggcct
     3601 gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc
     3721 ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgtga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaaac acgtacaagc actcgacctt tcaatgcgtt ttcgctaata
     4021 gttgacagat ttatccgctc catcgcgggc ggataatctc cccttcccca actttcttcg
     4081 taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc ggaatctgct
     4141 ttcccgtttc gttttttgcc cctcgcctgt gctgatggcg tttgccaaag tagcgccaac
     4201 taaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccgc tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacaccgggt tgggataacg
     4561 caaacggcgt caccggctgg acgcgcaact gcaatattca aagtgacagc gcattgcatc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac tgccctgtgt ctgcgttatc atggagtctt gctggatgcc agcgaagagg
     4741 tggttcatgt tgcggtagtc gatgcacctt cgcatgagct actggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctaactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgagccag gcactggatg tcaacacgct tggaatgcag ccgttacaac
     5281 tggcggactt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 caggcagcgg caaaacggtc acgctttata gtgccctgca aacgctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccgga ctcacctttc agggcgtgtt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacagcagag atcgctatta
     5581 aagcggcgca aactggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgcaaac tttgcccaca ttgtcgccgg cagcaagggg
     5761 agcccatcca cattccagac aatgtatggc catcgccgct gccccactgg caggcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc cttatttgaa gttctgccca
     5881 taacgccggt cattcgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcacgaca ggcgggtatg cgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgtg ctttgctgct catggcacta cagcaacaga tggttacccc acttagcctg
     6181 aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctactcaa agccgggtta acgctttctg aagggctggc actgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa
     6361 cagggcattg ctttttccaa tgccttatta ccctggtcag aggtatttcc gccactctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag tgaaatcagc gttacgttat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggagctggc tgctggtgtt gttcggcttt
     6721 ctgctggcga tagccaataa gttgctgatg cgccgaccga cctggcttat agtgcggcag
     6781 aaattgctgt tacgcatccc gattatgggt tcactgatgc ggggacaaaa actcacgcag
     6841 atttttacga ttctggcgct gacacaaagt gcaggcatta cttttttgca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatcagta acggtcatcc catctggctg gcgctaaaaa ataccgggga gtttagcccg
     7021 ctctgtttgc aattagtgag aacaggagag gcatccggct cgctggacct catgttagac
     7081 aacctcgccc atcatcatcg ggataacaca atggcgctgg cggataacct cgcagcctta
     7141 ctggaaccgg cgttgttgat cataacggga ggaattatcg gtacgctggt ggtggcgatg
     7201 tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg ctggcgtgtt
     7261 gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct tgttcctgca
     7321 cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
     7381 aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
     7441 gcagcttaac ggttttacct tctgctgcgc gatattccgc aacgccgcca acgtgacgtt
     7501 tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7561 cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
     7621 caccgccgcc aaaggctttt gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga
     7681 tcattccgcc cagaccgtgc gccgcatcgg cacattcgat taccgcagaa agctgcggat
     7741 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7801 cgatatcagc accggagagg ataagctcct cacacatttc accagtcact acgttgccag
     7861 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7921 cggaataacc attcgccaca tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7981 tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc acatgtttca
     8041 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8101 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtacctacgg
     8161 tgtccatatt tgcggcgata attggcacgc cggaccagct ctgacctgaa tgtttgaagg
     8221 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8281 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8341 ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg cactaatcaa
     8401 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8461 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgca ctcattttta acgttttgat
     8521 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8581 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8641 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8701 gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg ccgggccttg
     8761 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
     8821 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactgtgg
     8881 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8941 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt aactcgcgag
     9001 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc cgtggcagat
     9061 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9121 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9181 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9241 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9301 tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9361 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cctggcgtgg
     9421 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9481 ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc
     9541 gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9601 atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc ctgaacccgc
     9661 tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggctccc ttccgtaaac
     9721 aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9781 atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc cgttttgcca
     9841 ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg gatttcgaac
     9901 tggcctgttg ctaaggagtg aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9961 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
    10021 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10081 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct gatgcc
//
LOCUS       DAZFRJ010000157        10051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000157 DAZFRJ010000000
VERSION     DAZFRJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.159"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            431..1408
                     /gene="glaH"
                     /locus_tag="ACHKUN_003624"
     CDS             431..1408
                     /gene="glaH"
                     /locus_tag="ACHKUN_003624"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO6661818.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1428..2696
                     /gene="lhgO"
                     /locus_tag="ACHKUN_003625"
     CDS             1428..2696
                     /gene="lhgO"
                     /locus_tag="ACHKUN_003625"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO6661819.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2719..4167
                     /gene="gabD"
                     /locus_tag="ACHKUN_003626"
     CDS             2719..4167
                     /gene="gabD"
                     /locus_tag="ACHKUN_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6661820.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4181..5461
                     /gene="gabT"
                     /locus_tag="ACHKUN_003627"
     CDS             4181..5461
                     /gene="gabT"
                     /locus_tag="ACHKUN_003627"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO6661821.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5772..7172
                     /gene="gabP"
                     /locus_tag="ACHKUN_003628"
     CDS             5772..7172
                     /gene="gabP"
                     /locus_tag="ACHKUN_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO6661822.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7193..7855
                     /gene="csiR"
                     /locus_tag="ACHKUN_003629"
     CDS             7193..7855
                     /gene="csiR"
                     /locus_tag="ACHKUN_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO6661823.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7856..8305)
                     /gene="kbp"
                     /locus_tag="ACHKUN_003630"
     CDS             complement(7856..8305)
                     /gene="kbp"
                     /locus_tag="ACHKUN_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO6661824.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8389..8547)
                     /gene="yqaE"
                     /locus_tag="ACHKUN_003631"
     CDS             complement(8389..8547)
                     /gene="yqaE"
                     /locus_tag="ACHKUN_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6661825.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8730..9029
                     /gene="ygaV"
                     /locus_tag="ACHKUN_003632"
     CDS             8730..9029
                     /gene="ygaV"
                     /locus_tag="ACHKUN_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6661826.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9039..9563
                     /gene="ygaP"
                     /locus_tag="ACHKUN_003633"
     CDS             9039..9563
                     /gene="ygaP"
                     /locus_tag="ACHKUN_003633"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO6661827.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9610..10014)
                     /gene="stpA"
                     /locus_tag="ACHKUN_003634"
     CDS             complement(9610..10014)
                     /gene="stpA"
                     /locus_tag="ACHKUN_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO6661828.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2321 a         2580 c         2732 g         2418 t
ORIGIN      
        1 cgcttagagg cggaactcta aatatcaata ttcctgggcg tagcgctctt cttttaggga
       61 aaacaggaga accgccgaac tatctctatt tataatttaa tgttatatct gccccgataa
      121 aacggggcag ataatatgtt tagtttacta acggtcattt tgcagtgaag ccatttactg
      181 ttttttatcg accagataat ctgttctcta atgttaactc cccctaacct gttgctttag
      241 ttattcattt cctgtctcac tttgccttaa taccctacgt taaatgttac taatttgttg
      301 cttttgatca caataagaaa acaatatgtc gcttttgtgc gcatttttca gaaatgtaga
      361 tatttttaga ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg
      421 atcgcttctg atgaatgcac tgaccgccgt acacaataac gctgtcgatt caggccagga
      481 ctatagcgga ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt
      541 caccgaacag acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct
      601 ggagtacaaa tcgtttctgc gttttcgggt aggcaaaatt cttgacgatc tgtgtgcgaa
      661 tcagctgcaa ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat
      721 caatgcggtg ggtatcgatg atgtggcgca ggcagatgag atggtgaagc tggcaacggc
      781 ggtagcgcat ctgattggtc gctcaaattt cgatgcgatg agcggtcagt attacgcgcg
      841 tttcgtggtg aaaaatgtcg ataactcaga cagctatctg cgtcagccgc accgcgtgat
      901 ggaactgcac aacgacggca cgtatgtcga agagatcacc gattacgtgc tgatgatgaa
      961 aatcgatgag caaaacatgc agggtggaaa ttcgttgctg ctgcatctcg atgactggga
     1021 acatctggac cactatttcc gccatccact ggcgcgtcgc ccgatgcgct ttgccgcgcc
     1081 gccgagcaaa aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg
     1141 tcgcccggtg atgcgctata tcgaccagtt cgtccagcca aaagattttg aagaaggcgt
     1201 gtggttgagc gagctttccg atgccattga aaccagcaaa ggcattcttt ctgtacccgt
     1261 tcccgttggc aaattcctgt tgataaacaa cctgttctgg ctgcacggtc gcgaccgctt
     1321 tactccgcac ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc
     1381 cactcaccac taccagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg
     1441 tgattattgg cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtttatc
     1501 cggatgcccg cattgcgttg ctggaaaaag agtccggccc ggcctgtcac cagacgggcc
     1561 acaacagcgg cgtgatccat gccggggtct attacacgcc cggcagcctg aaggcgcagt
     1621 tttgcctggc gggaaaccgc gccaccaaag ccttttgcga tcaaaacgac attcgctatg
     1681 acaactgcgg caagatgctg gtcgccacgt ccgatctcga aatggaacgg atgcgcgcgt
     1741 tgtgggaacg cacggcggcg aacggtatcg agcgcgagtg gttaaacgcc gaggaactgc
     1801 gcgagcgcga accgaatatc accgggctcg gcggcatttt tgtgccgtcc agcggcattg
     1861 tcagctatcg cgaagtcaca gcggcaatgg caaaaatctt ccaggccaga ggcggtgaga
     1921 ttatttataa cgccgaagtc agcgccctca gtgagcataa aaacggcgtg gtcgtccgaa
     1981 cccgtcaggg cggcgaatat gaagcatcga cgctgattag ctgttccggg ctgatggctg
     2041 accggctggt gaaaatgctc ggcctcgaac cgggttttat tatctgcccg ttccgtggcg
     2101 agtatttccg ccttgcgccg gagcataacc agattgttaa ccatctgatt taccccattc
     2161 ccgacccggc aatgccattt ttgggcgtgc atctcacccg aatgatcgat ggcagcgtca
     2221 ccgtcgggcc aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag cgcgacttct
     2281 catttagcga cacgctggag attttgggct cgtcggggat tcgccgggtg ctgcaaaacc
     2341 atctacgctc aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcgcc
     2401 tggtgcaaaa gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg
     2461 tgcgggcgca ggcagtatcg ccggacggca agctgattga cgattttctg tttgtcacca
     2521 ccccacgcac gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta
     2581 ttggtgcgca tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac
     2641 gcacgctgcg agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt
     2701 tgaaaacagg acgtaacaat gaaacttaac gacagtaact tattccgcca gcaggcgttg
     2761 attaacgggg aatggctgga cgccaacaat ggtgaagtca tcgacgtcac caatccggcg
     2821 aacggcgaca agctgggtag cgtgccgaaa atgggcgctg atgaaacccg cgccgctatc
     2881 gacgccgcca accgcgccct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccaac
     2941 attctgcgca actggttcaa tttgatgatg gagcatcagg acgatttagc gcgcctgatg
     3001 accctcgaac agggcaaacc actggccgaa gcgaaaggtg aaatcagcta cgccgcctct
     3061 tttattgagt ggtttgccga agaaggcaaa cgcatttatg gcgacaccat tcccggtcat
     3121 caggccgata aacgcctgat tgttatcaag cagccgattg gcgttaccgc agctatcacg
     3181 ccgtggaact tcccggcggc gatgattacc cgtaaagccg gtccggcgct ggcggcaggc
     3241 tgcaccatgg tgctgaagcc cgccagtcag acaccgttct ctgcgctggc gctggcggag
     3301 ctggcgattc gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg ttcggcaggc
     3361 gcggtcggta atgaactgac cagcaacccg atggtacgca aactgtcgtt taccggttcg
     3421 accgaaattg gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg
     3481 gagctgggcg gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga taaagccgtg
     3541 gaaggcgcgc tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt
     3601 ttatacgtgc aggacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc
     3661 aaactgcaca tcggcgacgg gctggataaa ggcgtcacca tcgggccgct gatcgatgaa
     3721 aaagcggtag caaaagtgga agagcatatt gccgatgcgc tggagaaagg tgcgcgtgtg
     3781 gtttgcggcg gtaaagcgga tgaacgcggc ggcaacttct tccagccgac cattctggtg
     3841 gacgtgccgg ccaacgccaa agtgtcgaaa gaagagacgt tcggccccct cgccccgctg
     3901 ttccgcttta aagatgaagc cgatgtgatt gcgcaagcca atgacaccga gtttggcctt
     3961 gccgcctatt tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcgctggag
     4021 tacggcatcg tcggcatcaa taccggcatt atttccaatg aagtggcccc gttcggcggc
     4081 atcaaagcct cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa
     4141 atcaaatata tgtgcatcgg tctttaactg gagaatgcga atgaacagca ataaagagtt
     4201 aatgcagcgc cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc
     4261 tgaccgcgcg gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc
     4321 gggcgggatt gcggtgctca ataccgggca cctgcatccg aaggtggtgg ccgcggtgga
     4381 agcgcagttg aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct
     4441 ggagctgtgc gagattatga atcagaaggt gccgggcgat ttcgccaaga aaacgctgct
     4501 ggttacgacc ggttccgaag cggtggaaaa cgcggtaaaa atcgcccgcg ccgccaccaa
     4561 acgtagcggc accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc
     4621 gctgaccggc aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta
     4681 tcgcgcgctt tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat
     4741 ccaccggatc ttcaaaaatg atgccgcgcc ggaagatatc gccgccatcg tgattgagcc
     4801 ggttcagggc gaaggcggtt tctacgccgc gtcgccagcc tttatgcagc gtttacgcgc
     4861 gctgtgtgac gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg
     4921 taccggcacg ctgtttgcga tggagcagat gggcgttgcg ccggatctca ctacctttgc
     4981 gaaatcgatc gcgggcggct tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga
     5041 tgccgtcgct ccaggcggtc tgggcggcac ctatgccggt aacccgattg cctgcgtggc
     5101 ggcgctggaa gtgttgaagg tgtttgagca ggaaaatctg ctgcaaaaag ccaacgatct
     5161 ggggcagaag ctaaaagacg gattgttggc gatagccgaa aaacacccgg agatcggcga
     5221 cgtacgcggg ctgggagcga tgattgccat cgagctgttt gaagatggcg atcacagcaa
     5281 accggacgca aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg gcctgattct
     5341 tctctcctgc ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga
     5401 cgctcagatc cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagcagta
     5461 gctatgtccg tttgtaagag ttaaacgttg ctaagggaat gataacgatg cagtaaaaaa
     5521 taataacgat gaggcgatcg acaaagttaa aaacgactgc ggactgtccc ctcgctcctc
     5581 tggggagagg gttagggtga ggggtaatca cgcggctaca ccctcacccc ggccctctcc
     5641 ctaagggaga gggagaaaac tctcccgctt tcgtttttaa gattttgttg accgtctcaa
     5701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     5761 gaggattcag gatggggcaa tcatcgcaac cacatgagtt aggcggcggg ctgaagtcac
     5821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     5881 gcgtcgccat cgccgaagcg ggcccggcgg tattactggc ctatctgttc gccggattac
     5941 tggtggttat gattatgcgg atgttggcgg aaatggcagt tgccacgccc gataccggtt
     6001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggttatacc atcggctggc
     6061 tgtactggtg gttttgggta ctggttatcc cgctggaagc caacatcgcc gctatgatcc
     6121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     6181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     6241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     6301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     6361 ccaacggctt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     6421 cagaaattgt caccattgcc gccgcggaat ccgacacgcc ggaaaaacat attgtccgcg
     6481 ctaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtag
     6541 tggcgttaat accgtggaat atgccggggc tgaaagccgt tggttcttat cgctcggttc
     6601 tggaattgct caatattccc catgcgaaat taatcatgga ctgcgtgata ttactttccg
     6661 taaccagctg tctgaactcg gcgctgtata ccgcgtcaag gatgctctac tccttaagcc
     6721 gtcgcggtga tgcgcccgcg ctaatgggca aaatcaaccg cagtaaaacc ccgtatgtgg
     6781 cggtgttact ctccaccgga gcggcatttt taacggtggt ggtgaactat tacgcacctg
     6841 cgaaagtgtt taaattcctg atagacagct ccggcgctat cgccctgctg gtttatttag
     6901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     6961 gcttgcgcat gtggctttat ccctggctca cctggctggt catcggcttt attacctttg
     7021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
     7081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
     7141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
     7201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
     7261 ggatgaaaaa ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt
     7321 gcgggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
     7381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
     7441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
     7501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
     7561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
     7621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gctatcgatt
     7681 tatctggctg cggcgaacgg tgctttcggt agaaatgctg gaggataaac acgatcagca
     7741 ccagaccctg actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg
     7801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
     7861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
     7921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
     7981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
     8041 ttttcacctg atcatcgaca ctggcaatac cggaaatatt cccaaccgca acaaggattt
     8101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
     8161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
     8221 tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat
     8281 ctttcacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
     8341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
     8401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
     8461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat
     8521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
     8581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta
     8641 cgcttttact ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt
     8701 tacttaattt agaaaatact taaataaata tgactgaact cgcgcaatta caggccagtg
     8761 ccgaacaggc agcggcctta ttgaaagcaa tgagccatcc taaacgattg ctgattctgt
     8821 gcatgcttag cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga
     8881 gtgcctctgc gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc
     8941 aacgggatgc ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg
     9001 ccaccctgaa aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc
     9061 cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg
     9121 atgaatatct tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac
     9181 agtcaggtct tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta
     9241 aacgcaccag taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt
     9301 tactcgaaga tgggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat
     9361 ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg
     9421 gcgttgtact gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg
     9481 ccgggttact gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga
     9541 tgccgtggaa ccaacgaact tgagaagcga cgccggacgc gccctagcag cgacatccgg
     9601 cctcagtaat tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat
     9661 tggcttcggt gtacggccct gaccggtcca ggttttagtt tcaccgttaa catcggtgaa
     9721 tttatatttc gccggacgcg gctggcgttt tttaccagcg cgtggtgcag cagcagagct
     9781 attacctaat aactcttccg ggttaattcc gtcagccttc atcagctcca gccaggtgct
     9841 aattttttcc tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc
     9901 tttagtgaca accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg
     9961 agccatcgca cggagagtgc gaatgttatt taaactttgt aacattacgg acataataaa
    10021 gaaaaccttt taacgccaaa acaaaaaagt a
//
LOCUS       DAZFRJ010000158         9994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000158 DAZFRJ010000000
VERSION     DAZFRJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.160"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            167..376
                     /locus_tag="ACHKUN_003635"
     CDS             167..376
                     /locus_tag="ACHKUN_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661829.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            493..2814
                     /locus_tag="ACHKUN_003636"
     CDS             493..2814
                     /locus_tag="ACHKUN_003636"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6661830.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(2871..3206)
                     /gene="ymgD"
                     /locus_tag="ACHKUN_003637"
     CDS             complement(2871..3206)
                     /gene="ymgD"
                     /locus_tag="ACHKUN_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO6661831.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3210..3554)
                     /gene="ymgG"
                     /locus_tag="ACHKUN_003638"
     CDS             complement(3210..3554)
                     /gene="ymgG"
                     /locus_tag="ACHKUN_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO6661832.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3556..3729)
                     /gene="ymgI"
                     /locus_tag="ACHKUN_003639"
     CDS             complement(3556..3729)
                     /gene="ymgI"
                     /locus_tag="ACHKUN_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO6661833.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3830..4015
                     /gene="ymgJ"
                     /locus_tag="ACHKUN_003640"
     CDS             3830..4015
                     /gene="ymgJ"
                     /locus_tag="ACHKUN_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO6661834.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3976..4095
                     /locus_tag="ACHKUN_003641"
     CDS             3976..4095
                     /locus_tag="ACHKUN_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO6661835.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            4130..4474
                     /locus_tag="ACHKUN_003642"
                     /pseudo
     CDS             4130..4474
                     /locus_tag="ACHKUN_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4846..5112)
                     /gene="minE"
                     /locus_tag="ACHKUN_003643"
     CDS             complement(4846..5112)
                     /gene="minE"
                     /locus_tag="ACHKUN_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO6661836.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(5116..5928)
                     /gene="minD"
                     /locus_tag="ACHKUN_003644"
     CDS             complement(5116..5928)
                     /gene="minD"
                     /locus_tag="ACHKUN_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO6661837.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5952..6647)
                     /gene="minC"
                     /locus_tag="ACHKUN_003645"
     CDS             complement(5952..6647)
                     /gene="minC"
                     /locus_tag="ACHKUN_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO6661838.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7167..7535
                     /gene="ycgJ"
                     /locus_tag="ACHKUN_003646"
     CDS             7167..7535
                     /gene="ycgJ"
                     /locus_tag="ACHKUN_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO6661839.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(7638..8039)
                     /gene="pliG"
                     /locus_tag="ACHKUN_003647"
     CDS             complement(7638..8039)
                     /gene="pliG"
                     /locus_tag="ACHKUN_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO6661840.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8110..8268
                     /locus_tag="ACHKUN_003648"
     CDS             8110..8268
                     /locus_tag="ACHKUN_003648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661841.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            8280..8573
                     /locus_tag="ACHKUN_003649"
     CDS             8280..8573
                     /locus_tag="ACHKUN_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO6661842.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            8645..9304
                     /gene="ycgM"
                     /locus_tag="ACHKUN_003650"
     CDS             8645..9304
                     /gene="ycgM"
                     /locus_tag="ACHKUN_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO6661843.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            9396..9842
                     /gene="ycgN"
                     /locus_tag="ACHKUN_003651"
     CDS             9396..9842
                     /gene="ycgN"
                     /locus_tag="ACHKUN_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO6661844.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         2654 a         2184 c         2418 g         2738 t
ORIGIN      
        1 tgaaaaatga taatgccgac ttatttatca tttatatatt gtcgctgttt atctatttaa
       61 attttttgtg cttttgtttt tgtctttacc ctccgttttg gtaatctgaa cgcataggtt
      121 atttgaccag gaatgtttgt taatccaatg aatggatggt taatctatga aactgaaaaa
      181 actctccggg tttagtttgg gacttattgc tctggcggtg ggtaatgcat atgctgaaca
      241 gtttattcag cacgatgaat ataaacatgc tgtggcaata aaagatggaa aagtaaaaga
      301 tattgataat gcgctagtta taacaacggg taatggaagt tacgggtatc cgttgcagga
      361 gagggtagca agttaaccat taacaatggc gtcattgtaa caacgggcgg tttatatcct
      421 tctgacaata gtcctctaag cacagcatta acagctagcg caatcgtaag tgaatttggc
      481 ggtgctgtta ttttgcaggg taaaaacacg attgtcataa cgggggatta ctcaattggt
      541 ttgctaagtc aaacgagtgg aaacctgaat actgacacca taataagagt caactctgac
      601 ggttcggtta ccccctcttt ttctgacgga gatgatacat ttattgttac tgcgggtaat
      661 catgcagtcg gcgttcttgc gtgtgcatct cccggaagcg cgcgtgcgtg tgtatcttct
      721 cttgatgaag aaagtactac cgatacagga agtaatgaaa ataatgccat agcaaaactg
      781 gatatggcaa aaggggagat cacaacccac ggaacagaaa gttatgccgc gtacgctaat
      841 ggcactgtcg tcaaggctgg tgacacgctt gattatacca atgccagcgt tacattaacc
      901 gatgttgata tcaccacgca tggggacaat gctcatgcca tcgctgcccg ccaggggaca
      961 gtttcattta accagggaga aatttataca acgggtcctg acgccgcgac tgctaaaatt
     1021 tataatggcg gcacggtgac gctgaaaaat acatctgcag tcgcgcatca ggggtctgga
     1081 attgtgctgg agtcttccat aaatggtcag gaagcaacgg tagatatttt atctggtagc
     1141 tcactagggt cagcaaatga aatcctctac cataaaaatg agacgagtaa cgtgaccatt
     1201 acggatagtg aggtatcatc ggctgcagat gtttttatca ataatattaa aggtcatttg
     1261 accgtcgatg caactaattc aaaaataacg ggttcagcta atatttcaac agatgttaac
     1321 actcatacct atctgtcgct ttcagataat agtacctggg atattaaagc tgactcaacg
     1381 gtgagcaacc tcacggttga taatagtacg gtctatattt cccgggcaga tggacgggat
     1441 gtcgaaccaa caagattaac aataactgaa aattacgttg gtaataatgg cgtattgcat
     1501 ctcagaactg aattgggtga tgataattca gctacggata aagtcgttat taatggaaat
     1561 acctctggaa caacccgagt taaagttacc aatgcaggag gcagcggagc ttacacgtta
     1621 aatgggatag agattatcag cgttgagggg gaatcaaatg gggaatttat taaggattcg
     1681 aggattttcg ccggtgccta cgaatattca ttaacccgag gtaataccga agcgaccaat
     1741 aaaaactggt atctgactaa cttccaggca acgagcggcg gtgaaacaaa ctccggagga
     1801 agttcagcgc ctactgttgc gcctaccccc gtcctgcgcc tcgaagctgg aagttacgtc
     1861 gccaacctgg cagccgctaa cactcttttt gttatgcgtc tgaacgaccg tgcgggtgaa
     1921 atgcgctaca tcgatcctgt aactgaacag gagcgttcaa gccgactttg gctacgtcaa
     1981 attggcgggc ataatgcctg gcgtgacagc aacggacagt tgagaacgac ctcgcatcgc
     2041 tacgtctcgc agttaggggc cgagctgtta accggtggtt ttaccgacag tgacagttgg
     2101 cgtttgggag tgatggctgg ttatgcccgc gactacaact caactcattc cagcgtgtcg
     2161 gattatcgtt cgaaagggag tgtcagaggc tattgcgcag ggctgtatgc cacttggttt
     2221 gccgatgaca tcagtaaaaa aggcgcatac attgacgcct gggcgcaata tagctggttt
     2281 aaaaactcgg tgaaagggga tgaattagcc tatgaatcct atagtgcgaa aggcgcaacc
     2341 gtctcgctgg aagcgggtta cggctttgcc ctgaataaat cctttggtct ggaagcggcg
     2401 aaatacacgt ggatattcca gccacaggca caggctatct ggatgggcgt cgatcataat
     2461 gcgcacaccg aagccaatgg ctcacgtatt gagaatgacg caaataacaa catccagacc
     2521 cgacttggtt tccgcacctt tattcgtact caggagaaaa acagcggtcc gcacggtgac
     2581 gactttgaac cttttgttga aatgaactgg atccataaca gtaaagattt tgctgtctca
     2641 atgaacggtg tgaaagtcga acaagatggg gcgagtaatt tgggggaaat taaacttggc
     2701 gtaaatggca acctgaatcc agcggccagc gtctggggga atgtgggcgt gcagctgggt
     2761 gataatggct acaatgacac cgcagtgatg gtgggcctga aatataagtt ctgatcccgc
     2821 cgttagctaa aaaaccgcgt cgtattcatc gacgcggtac atatgaaata ttattttttg
     2881 ccgatagcac gcatggtgtc atcaattgcc gtgatcaaca gcatttgcgg gtctttagcg
     2941 caaacctgat tcagtttttc taccactttg gcgctcagtt ccggagattg ctcaattttt
     3001 aaatcacggc tggcaacgct ggcattaccc attaccgcaa caatttctgc aacctgtgcg
     3061 ctgtcagttt ttgccatttc gttggcttct gcgcaagtaa tataggtttc tgacggcaaa
     3121 ccgtttttaa tattgtagtc ctgcgcccag gtcattggtg cgaaaacaaa caggcccgcc
     3181 agtaaagcaa attttttcat catcattcct tatttcattt tacccagaat tgcaccaccc
     3241 gtaccgccaa tcacggcacc tttaatcgcc ccttcgaggc cattgccggt cagaacgcca
     3301 gtgacagcac caacggcggc acctactttt gcacctttac gcgcattttt accgtcgcgg
     3361 cctttttctg ttactgcacc aacaccagcg ccaacagctg cacctttcag tacgccatta
     3421 acaccattgc cagtaagtaa accaacgcct gcgcctagca atgcaccttt cgtggtgcga
     3481 ttcatatccg ccatcgctgg cgtggagcag aacaatgctg agataagccc gaaggcaagt
     3541 atttttttct tcaacttaga tgtccggtat taagtaagtt gcacacacaa taatttcgtc
     3601 ttcaattaag atctgcttaa ctaaagaacg ctcgctatta ttcagataat tcaaaatgag
     3661 cgtggctgtg atgataggaa ttatgttttt tacgtgaatg agaataatct taaatgagga
     3721 ataactcatt gattgacaat atttttattc aagaagtgtc attgactgtt aacgcaatgt
     3781 tgtaaaggta agataatctg atttatcaat attattgtgt gatttttatg tgagcagaag
     3841 atattcatca gcaacgatta cattagtcat tttattttgc cgacggcctc attgtcgaaa
     3901 gataagcgta cgacagtatt atctgaaaag agtgattttt tatccaacta cacttcagcg
     3961 cactgcgtgt aaaaaatgcc tctttcttat gcgggatatc atcatttcat catgatgtct
     4021 ttggtgagcg gtgaacacaa tacacctgcg ctgtctcttc aggatgaatc ccctggtctg
     4081 gtgcctgcgg gctgatgttg cagcagagct taggtcactt agacgctact atcatttatc
     4141 caatggcatg gaatcgaaat cagtcgatac ccgcagtata tatcgtgaac tgggtgcaac
     4201 gctgagttac aacatgcgtc tggggaacgg tatggaaatt gaaccctgcc tgaaggcggc
     4261 tgtgagcaaa gaatttgtcg atgataatcg ggtgaaagtg aatagtgacg gtaattttgt
     4321 caatgatttg tcgggcagac gtggaatata ccaggcaggt attaaagcct cattcagcag
     4381 tacgttaagc gggcatttcg gggtggggta tagccatggt gccggtgtgg aatccccgtg
     4441 gaatgcggtg gctggtgtga actggtcgtt ctgaccatca acgattaaac tgcgcttcgg
     4501 cgcagttttc gtttacaggg ggctaaaagg gaaaattctg gggcaaaaaa agcccgccag
     4561 ttacggcggg aaacctcatc ctatgggaga acaatgaata atgaaattgc ggggttatca
     4621 tcccccagtg tatccacact aacaatgagg ttatttactc aaccaggcat aaacattttg
     4681 ttctgtgcgt gggaacagcc ttaaggtgta aagggggagg tggaaatagc aatgaggagt
     4741 atcagcaaga atactcgccg ctttaccaca acgtggatga gagggatgaa aaactcaagg
     4801 cagaaatgac tctgccttga agataaatgc gtttttacag cgggcttatt tcagctcttc
     4861 tgcttccggt aaggtcacgt tcagctcaag aatagaaata tcgccatctt tttgctcaag
     4921 ctgtacggtt accatctcag gatcaatttg tacgtattta caaatgacct caagaatatc
     4981 tttacgcaac tgcggcagat aatgcggttc tgcatcgctg cgacggcgtt cagcaacaat
     5041 aatctgcagc cgttcttttg caatgttggc tgtgtttttc ttccgcgaga gaaagaaatc
     5101 gagtaatgcc ataacttatc ctccgaacaa gcgtttgagg aagcctttct tctcttcttc
     5161 aatgaagcgg aaaggacgtt cttctcccaa cagacgttct acggtatctg cgtaggcttt
     5221 acccgcatcg gcgttaatgt cgagaatgac cggttcaccc tggttagagg cgcgcaatac
     5281 tgattgatcc tctgggatca cgccgacgag tttgatgcgc aggatctcca gcacatcttc
     5341 catgctcagc atgtcacctc tgcttacgcg gcctgggtta tagcgcgtta acagcaggtg
     5401 ctctttaata ggctcttcgc cattttctgc gcggcgtgat ttcgacgcca gaatgcctaa
     5461 aatacggtca gagtcgcgta ctgaggagac ttccgggttg gtggtaataa tggcttcgtc
     5521 tgcaaaatag agtgccatta acgcaccggt ttcaatccct gccggggagt cacaaacgat
     5581 aaattcaaaa tccatcgctt tcagatcatc aagaactttg gcgacccctt cacgggtgag
     5641 ggcatcttta tcgcgtgttt gcgatgccgg aagaatatag agattttcag tacgcttatc
     5701 tttaattaac gcctgattta gcgttgcatc gccctgaatg acgttgacga aatcgtaaac
     5761 gacccggcgt tcacaaccca taatcaggtc gagattacgc aggccgatat caaaatctat
     5821 cacgacagtt ttctttccct tctgggccaa accagtggcg atggccgcgc tggaggttgt
     5881 cttaccaaca ccccctttgc ccgaagtaac aacaataatg cgtgccatag aaattccttg
     5941 ttaaaaaggg atcaatttaa cggttgaacg gtcaaagcgt tttcgactaa ctgcagtcgc
     6001 gccgctttgc cataaaattc tgctgggatt tgatcactca gccagtattc acctgcgatg
     6061 gacaccagtt ccgccatcag gttcgtacaa aatatttgcg tttcccggtc accacttgcc
     6121 cctgccagcg cacgaccgcg catcatgcca tagacatgaa tgttaccatc ggcaatcaat
     6181 tcggccccag cgctaacgtg gcttgtaaca atcagatcac attgtggagc ataaatacgc
     6241 tgaccggaac gcaccggggt atctattaaa cgcgtttttg tgaccggcgt tgtattttgc
     6301 gctggagcct gcggtgcggg agctggacgt ggcgcttttt cctttccttc cgtcaggata
     6361 ggcagcccca ttttttcaat ttcggcttta agttgcgcat ctttgcagcc acttacgcca
     6421 ataacccgca aaccggttgc cgaaaccgcc ttatgcatcg ctgaccagtt taccgggtct
     6481 tccagtgcac tgacgttgag tacaacgggg gcatgtttta aaaatgcggg ggcctgagcg
     6541 attttgtctt ccagcgcctg atggataacc ttaggttctg cctcatgcag atgaaccaca
     6601 gataaagtga agctactgcc tttaagctcg attggcgtgt ttgacatcct ggccttactc
     6661 aattagctat taatcatcgc cagcgcgcga tgatgttccg aagactataa ggcatgttat
     6721 agtctggatt atattgaggc aagtcaccct cccatttatt cagagtaaaa gtctattctg
     6781 tgataaatgg cgctgattca tagcttaaaa aatacccttg tcaatcaacc cattgccgtc
     6841 gtacttttga ttcttcttat ttacgcttcc tttttccgca ccctaatcgt gacagcatct
     6901 ccagacctgt ttaaatatcc agtattgagg caagtgtatt ttttcaccac gccaggcttt
     6961 cttaactttc cattttttgc ggcatcatta aagaacagat acccgttatc ttaatatccg
     7021 ccacagaatt gctctatcat agaaaccgca gaagacgtct gatacttctg tcaggtcatc
     7081 gtaactcgtc caggtcagcg gttttgatta cggattgatg acgaaatgag cataagtgtt
     7141 gtacggttgg attatcagga tgtgctatga atatgatgcg tattatttat atcggattgt
     7201 caggtgtggg gatgatgttc tcatcaatgg cgtctggaca tgacgctagc ggacttcaat
     7261 ctccggcgtg cggcgttgtt tgcgacccgt atatttgtgt gaactcagat ggcatttccc
     7321 cagagttaac aaggaaatat ctcggcgaaa aagccgctga aaacttacaa tcattacaag
     7381 gctacgatcc cagcgaattt acattcgcta acggtgtatt ttgcgatgtt aaagaaaaat
     7441 tatgtcgtga tgatcgttat tttggtgtag atggtaagcg gagtggaaaa atcaatcaaa
     7501 ccaccacaaa gatgttattt atgtgtcgtg agtgatgtct tgtttacatt atgttctatt
     7561 cgtcattaac ctcttgagcg acagagagga gtggggcgtt aacgtttatg acggggagag
     7621 tccccgtcgg ttgacattta cttaatttga atatcgacgt tatatttttt agttttgttt
     7681 ttacgggcgt cgtttcgggt ttgcaacact cgcagctcat atttgccaga agcagggagt
     7741 gagtattgtc cgtgactatc gagttctggg gaatatctgg ataaatcaac tgaatcatcg
     7801 attcctggcc cgaagaggta agtatcagcg ccttcattag agatactcac atgcactttc
     7861 tgaccttttt tggcatagaa ggtatatgta tcgtaatcgt atccctttat ttcgccggag
     7921 tactgagcgc tgctatggcc ttttctgaac tcaacattga cattcttacc cgcagcaaag
     7981 ctcgtggagg ttagcagtgc aaacagcaat acagccttac tgatgctctt gattttcatt
     8041 ggtagtctcc tgttcagaat gagtacgatg aactgttata atataacaat ccctaacggg
     8101 agaattccag tggctttcct gcgttcggta aaatcccggc gcggcatttt tgccgggggg
     8161 attacttata aaggggcatg gactcaacaa accgtagaaa tctacagctg gctggcttgt
     8221 ggcgcgggtt tcattgatgt tagcttatgc caaaacctgg catcttaaag agtaaaagta
     8281 tgttttgtgt gatttatcga agcagcaagc gtgaccagac ctatttatat gtcgaaaaaa
     8341 aagacgattt ttcgcgtgtt cctgaggaac tgatgaaagg ttttggtcag cctcagttag
     8401 cgatgattct gccgttggat gggcggaaga aactggtcaa tgctgatatt gaaaaggtta
     8461 aactggcatt aaccgagcaa ggttactatt tgcagttacc gccaccaccc gaagatttgc
     8521 tgaagcaaca tctttccgtc atggggcaga aaacagacga cactaacaaa taaccgatat
     8581 ccggcggtgg cattatcttt gtcggcgcgg gttttcatat ccacgataag gtgaggggaa
     8641 cgttatgtat caacatcaca actggcaagg tgcgctgctg gattatccgg tgagtaaagt
     8701 agtctgtgtt ggcagtaact atgccaaaca tattaaagag atgggcagcg cagtgcccga
     8761 agagccagtg ctgtttatta aaccagaaac ggcactgtgc gatctgcggc agccattggc
     8821 gatcccatcc gatttcggtt cagttcatca tgaagtcgaa ctggcggtgt tgattggcgc
     8881 gacgctgcgt caggctacgg aagagcatgt ccgcaaagcc attgccggtt atggcgtggc
     8941 gctcgatctg acattgcgtg atgttcaggg aaaaatgaag aaagccgggc agccgtggga
     9001 aaaggctaaa gcgtttgata actcttgtcc gctttccggg tttattcccg cggcggaatt
     9061 taccggcgac ccgcaaaata caacgctggg cctgagcgta aacggcgagc aacgtcagca
     9121 aggtacgact gcggacatga tccataaaat tgttccgctg atcgcttata tgagcaagtt
     9181 ttttaccctc aaggccggtg acgttgtgct gacaggcacg cctgatggcg tcggcccgtt
     9241 gcaaagcggt gatgagctga cagtcacttt cgatgggcat tctttggcaa ctcgcgtttt
     9301 gtaatacttt ttgccgcctg aaagcggcgg caaaacttgc atcgctgtgc cagactggtt
     9361 ataaggtgcg ttttaacgta atggcggaac acctgatgag cgatgtacct ttctggcaaa
     9421 gtaaaaccct ggacgaaatg agcgatgcgg aatgggagtc gttgtgtgat ggttgcggtc
     9481 agtgttgcct gcataaactg atggatgaag acaccgacga aatctacttc actaacgtcg
     9541 cctgtcgcca gctcaatatt aaaacctgtc aatgtcggaa ctacgaacgt cgtttcgagt
     9601 ttgaacccga ctgcattaaa ttaacccgtg aaaatctgcc aacattcgaa tggctgccaa
     9661 tgacctgcgc ttatcggttg ctggcggaag gtaaagattt acctgcgtgg catccgctac
     9721 ttacgggttc gaaagcggca atgcatggtg aacgtatctc tgtgcgtcat atcgcagtga
     9781 aagaatcaga agtgattgac tggcaggatc atatcttaaa taaacctgac tgggcgcagt
     9841 gataaattta atagatgttg atgggggctt tcttaatata cgtctggatg ctcgccattt
     9901 cgtagccctg tttttgaaag cgctctgtca taatgagagt tacccgatac cttattatca
     9961 acaggtaact ctcaatcttt aaaaaacgtc tgtg
//
LOCUS       DAZFRJ010000159         9973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000159 DAZFRJ010000000
VERSION     DAZFRJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.161"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..173
                     /locus_tag="ACHKUN_003652"
     CDS             3..173
                     /locus_tag="ACHKUN_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661845.1"
                     /translation="MVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLTPPCIYA
                     IYKGLSFTKDKK"
     gene            170..997
                     /locus_tag="ACHKUN_003653"
     CDS             170..997
                     /locus_tag="ACHKUN_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6661846.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            1038..1409
                     /locus_tag="ACHKUN_003654"
     CDS             1038..1409
                     /locus_tag="ACHKUN_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO6661847.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            1441..1545
                     /locus_tag="ACHKUN_003655"
                     /pseudo
     CDS             1441..1545
                     /locus_tag="ACHKUN_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            1601..1855
                     /gene="xisR"
                     /locus_tag="ACHKUN_003656"
     CDS             1601..1855
                     /gene="xisR"
                     /locus_tag="ACHKUN_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6661848.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            1889..3175
                     /locus_tag="ACHKUN_003657"
     CDS             1889..3175
                     /locus_tag="ACHKUN_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6661849.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            3159..3956
                     /gene="yecE"
                     /locus_tag="ACHKUN_003658"
     CDS             3159..3956
                     /gene="yecE"
                     /locus_tag="ACHKUN_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO6661850.1"
                     /translation="MEPDSEIISLNISCFSGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            4009..4404
                     /gene="yecN"
                     /locus_tag="ACHKUN_003659"
     CDS             4009..4404
                     /gene="yecN"
                     /locus_tag="ACHKUN_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO6661851.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            4445..5188
                     /gene="cmoA"
                     /locus_tag="ACHKUN_003660"
     CDS             4445..5188
                     /gene="cmoA"
                     /locus_tag="ACHKUN_003660"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO6661852.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5185..6156
                     /gene="cmoB"
                     /locus_tag="ACHKUN_003661"
     CDS             5185..6156
                     /gene="cmoB"
                     /locus_tag="ACHKUN_003661"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO6661853.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(6321..8750)
                     /gene="torZ"
                     /locus_tag="ACHKUN_003662"
     CDS             complement(6321..8750)
                     /gene="torZ"
                     /locus_tag="ACHKUN_003662"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO6661854.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIRVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(8775..9875)
                     /gene="torY"
                     /locus_tag="ACHKUN_003663"
     CDS             complement(8775..9875)
                     /gene="torY"
                     /locus_tag="ACHKUN_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO6661855.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2502 a         2521 c         2374 g         2576 t
ORIGIN      
        1 caatagtaat tttactggca attatcgcat gcatttacgg attactcgtt ccgttcctta
       61 tatccatgaa ggatacgata gcagttactt ctggctttgc actggcgttt ctgaccccgc
      121 cctgcattta tgccatttac aagggtcttt ctttcactaa ggataaaaaa tgaaaaaaat
      181 tatttttgct ttagccattg ttctgccgac cattggcctt gtcggctgcg atcgcgttga
      241 acccggtaat gttggcatca aagtaaataa actgggcgac gataaaggcg tcggtgaggt
      301 ggtcggtgtt ggtcgctact ggactggctg gaatactgaa gtttacatct tcccaacctt
      361 caaacaaatg aagacctacg atgagccgtt cagcttccag atgagtgacg gcacaaccat
      421 cggctatcac atcggtgttg cctacaaagt tgatccatcc aaagttacca cggtgtttca
      481 gacctaccgc aaaggcgtgg atgacattac cgccactgac ctgcgccaga agatcgccga
      541 cgcactcaat cgactggcca gcaaaatgac caccgacaaa tttatcgacg gcggcaagtc
      601 tgaactactg gatgccgcac ttaaagacat tcaggaagag atgaccccca tcggcgttca
      661 ggtaatgagc ctctcttatg taggtaaacc ggaatacccg ccaaccgtta tcgacagcat
      721 taacgccaaa gtcacggcaa accagaaaac cctgcagcgc gaacaggaag tcaagcaacg
      781 tgaagcggag gccaacatgc tgcgtgcaga agctgccgga caggctgatg ccattcgaac
      841 aaaagccctg gcagaagccg atgccattcg tttacgcggc gaagctctgc gccagaaccc
      901 tggcgtcatg gagctggaag ccatcaacaa gtggaacggt acactgccgc aatacatgac
      961 cagtggtgcc aatacaccat ttatccagat taaataactt acacgcccgg caaaccgccg
     1021 ggttaatgga aaatcagatg aacaaccaga atactcaacc gcaaataatg aactatgacc
     1081 cgaatctgac gtcgtgcgga cgcatggcaa aacaaaccgt tcgattaact ttcggactat
     1141 gggaataccg cgaaacattc gaagttactg tcggcggcaa tctgaccgga ctggatgtta
     1201 tcaattgcgc tattgaaagc ctgtacgcaa cgctgcctta tgaagaagtc ctggatgagc
     1261 gcacagggaa aacggatatc atggccacca ttaatattgg cgaactgata tgccaggatg
     1321 aagacctgtc cggggaactc tggcttgccg ggatgcttat ctcagcagaa attatcagca
     1381 ttgaacccgc tacaaacata cggctctgaa gttctcacta ttcagagagc aggagaaaaa
     1441 atgttcgctc tgattaatca gggacaactg tataccgaca gtgccggtta cccggtaaaa
     1501 attgttcgct gcataaacaa caccgtgttg tacagaagat gtgaagcgaa tgtttgaagt
     1561 gtggtgccac tgccgtggat gaaagtttta tgaggttggc atgcagacaa tcatctatca
     1621 gataaccccc agcaaatggt gtacggagag agtcctcatt gcatcaacag ggctaaagcc
     1681 tggcaccatc gagcgagcca gaagaaagtc atggatgcag ggaaaagaat accgccatta
     1741 cgctgtagaa ggtgatccgg ggcattacag tgaatgcctg tacaacatcg aagaaattat
     1801 gcgatggatc gaaaaccaga aacaaccagg tgccaaaaat gcaagttccg gttaacctgt
     1861 taatgctcct ggacgtctgg gaggttttat gagtaacgca tcatacccga caggcgttga
     1921 aaaccatgga ggatcactcc gtatatggtt tcactataat ggcaaacgtg tcagagaaaa
     1981 cctcggtgtt cctgacaccg ccaaaaaccg gaagatcgct ggtgagcttc gcacttctgt
     2041 ttgttttgca atcagaatgg ggagtttcga ctacgccgcg cagttcccta attcccctaa
     2101 cctgaaacac tttggtctgg gaaaaagaga gataaccgtt aaggcacttt cggaaaaatg
     2161 gttggacctt aagaaaattg agataggaag taatgcattc agtcggtatc aatccgtggt
     2221 gagaaacatg cttcctcgca taggggaaaa acgtcttgct tcgtcggtaa caaaggaaga
     2281 tttactgttt atcaggaaag atttattgac cgggtatcat aatctttcta acggaaaaac
     2341 aacgccgatt aaagggaggt cagtagttac ggttaattac tacatgacga caattgcagg
     2401 aatgtttcaa tttgcggctg ataacggcta tatcgtgtca aacccattta acggcctgac
     2461 accattaaag agatccagaa cagaaccaga tccgctcaca cgtgacgaat ttattcgttt
     2521 tattgatgcc tgtcaccatc aacaaacaaa aaacctgtgg tccttagcag tatacacagg
     2581 cattcgtcac ggtgagctaa tatctctcgc ctgggaggat attgatttaa aagctaaaac
     2641 aatgactatc cgtcgtaatt atacaaaact cggggagttc actctaccaa aaacagaagc
     2701 gggaactgat cgtgttattc atcttgttca accagctgtt gatgccctga aaagccaggc
     2761 tgaaatgaca agacttggcc ctcagtatca aattgacgtc aagcttcggg agttcggtcg
     2821 caccgcacgc catgaatgca cgtttgtttt taatccgcaa ctggtaaaaa aatgccagca
     2881 agtcggtcac cactataaag cagattccat cagagattcc tgggcatctg cattaaggcg
     2941 agcaggactg cggcacagaa aagcctatca gtctaggcat acttatgcct gctgggcatt
     3001 atcggcagga gcgaatccaa gcttcatagc aaaccaaatg ggccatgcaa atgcacaaat
     3061 ggtattcaac gtttacggag catggatgaa agataacaat atcgggcaaa tagaactact
     3121 caataagcag ttgacggaga gtgtcccata catgccccat agagccagac tctgaaataa
     3181 tatctttaaa tatcagttgt tttagtggta gttgccgtca cattaactgc gtggagggca
     3241 acaccacgct ttacgccctg ccgaaacccg aggttgtcct gcgctggcgt gaacagacca
     3301 cagatgactt ccgcttctgt tttaagtttc cggcgaccat ttcgcatcag gcagcattac
     3361 gacattgcga tgatttagtg actgaatttt tgacccgcat gtcaccgttg gctccgcgca
     3421 ttgggcaata ctggctgcaa ctgcctgcca cattcggccc acgggagctg cctgcgcttt
     3481 ggcattttct cgattctctc cctggcgaat ttaattatgg tgtggaagtc cgccatccac
     3541 agtttttcgc caaaggggaa gaggaacaaa cgcttaatcg cggtttacat cagcgcggcg
     3601 ttaatcgggt gattttagac agccgcccgg ttcatgcagc atgtccacac agtgaagcta
     3661 ttcgcgacgc tcaacgaaaa aaacctaaag ttccggtaca tgctgtactg acggcgacaa
     3721 atccgctgat ccgttttatc ggtagtgatg atatgacgca aaaccgggaa ttatttcagg
     3781 tctggttaca gaaattagcg cagtggcatc agaccactac gccttatctt tttttacata
     3841 cgccagacat tgcccaggcc ccggaactgg tacataccct gtgggaagac ttacgtaaaa
     3901 cgcttccaga gatcggagca gttccggcta ttccacagca atcttctctt ttctgaattt
     3961 gccacctatc atagacaggt gccatcggcc attttaaagg gagtttgtat ggtaagcgcg
     4021 ctatatgccg ttttaagtgc gttgttatta atgaagttct cttttgatgt cgttcgcttg
     4081 cgtttgcagt accgcgttgc ctatggtgac ggtggtttta gcgaactgca aagcgctatt
     4141 cgcattcatg gtaacgcggt ggaatatatt cccatcgcga ttgtgctgat gctgtttatg
     4201 gaaatgaatg gcgcagaaac ctggatggtg catatttgcg gcatcgtttt gcttgctggt
     4261 cgtctgatgc attattacgg ttttcatcac cgtctgtttc gctggcgacg ttccggcatg
     4321 agcgccacct ggtgtgcgct gttgctgatg gtgctggcga atctttggta tatgccctgg
     4381 gagttggttt tctccctgcg ttagcgcaca atacgccact ttctttttcc cggattttta
     4441 cgttatgtct caccgcgaca cgctattttc tgcccctatc gccagactgg gcgactggac
     4501 ctttgatgaa cgggtagctg aagtcttccc ggatatgatc cagcgttccg ttcccggcta
     4561 ttccaatatt atttccatga ttggtatgtt agccgagcgc ttcgttcaac ctggtacgca
     4621 ggtttacgat ctgggttgtt ctctgggcgc ggcgacgctc tcggtgcgtc gcaacattca
     4681 tcatgataat tgcaaaatta ttgccatcga caactccccg gcgatgattg aacgctgccg
     4741 tcgtcatatt gacgcctata aagcccctac gccagtagac gtcattgaag gtgatattcg
     4801 cgatatcgcc attgaaaacg catcgatggt ggtgctgaat tttaccctgc aattcctgga
     4861 accttccgag cgccaggcat tactggataa aatttatcaa ggactgaacc cgggcggcgc
     4921 gctggtgctt tcggaaaaat tcagtttcga agatgccaaa gttggtgaac tgttgttcaa
     4981 catgcaccac gactttaaac gtgccaacgg ttacagcgaa ctggagatca gccagaagcg
     5041 cagcatgctg gaaaacgtga tgctgaccga ttccgtggaa acccataaag cacgcctgca
     5101 taaagccggt tttgagcata gcgagctgtg gttccagtgc tttaactttg gttcactggt
     5161 ggcattaaaa gcagaggacg ctgcatgatc gactttggta acttttattc tctgattgcc
     5221 aaaaatcatc tttcacactg gctcgaaacg ctgcccgcgc agattgctaa ctggcagcgc
     5281 gagcagcagc acgggctgtt taagcagtgg tccaacgcgg tggaatttct gcctgaaatt
     5341 aaaccgtatc gtctggattt attgcatagc gtaaccgccg aaagcgaaga gccactgagc
     5401 accgggcaaa ttaaacgcat tgaaacgctt atgcggaacc tgatgccatg gcgcaaaggg
     5461 ccgttctcac tgtatggcgt caacatcgat accgaatggc gttccgactg gaaatgggat
     5521 cgtgttctgc cccatctttc tgatttaacc ggacgcacca ttctggatgt cggctgcggc
     5581 agcggttatc acatgtggcg catgattggc gcaggcgcgc acctcgcggt ggggatcgac
     5641 cctacgcaac tgtttctgtg ccagtttgaa gcggtgcgta aactactggg taacgatcag
     5701 cgcgcacatt tgttaccgtt aggtattgaa cagcttccgg cactgaaagc ctttgatacc
     5761 gtcttttcga tgggcgtgct ttaccatcgc cgttcgccgc tggagcatct ctggcagttg
     5821 aaagatcaac tggtgaatga gggtgaactg gtgctggaaa cgctggttat tgatggcgat
     5881 gagaacacgg tgctggtacc aggcgatcgt tacgcgcaaa tgcgtaatgt ctatttcatc
     5941 ccttccgcgc tggcgctgaa aaactggctg aagaagtgtg gttttgtaga tatccgcgtt
     6001 gtggatgtgt gcgttaccac tactgaagag cagcgacgca ctgaatggat ggttaccgaa
     6061 tctctgtccg atttcctcga cccacatgat ccgagtaaga cagttgaagg ttatcctgcg
     6121 cctaaacgcg cggtgctgat tgcgcgcaag ccgtaaaggt ctggcattac tgccggatgc
     6181 ggcttgaatg ccttatccgg cctacaaacc cttgctaatt caatctattg cagggactat
     6241 gtaggcctga taagcatagc gcatcaggca gttttacgtt tgcataacct cagcgcccgt
     6301 ttccgggcgc tattcacttc ttacgcctga acagcaggct tatcaaatgc cgttaacttc
     6361 ggtgcgttac ccgtatattt ttcgatatac accagcgcag agttaccggc acaggcattt
     6421 gccagctgcg agctggggat atccgccgtt aacacgttcg cactgccgtt tttacacaag
     6481 ccgttttcca gatctggcca tgcaccttca tgcacgcata ccacgccttt tttgatcccg
     6541 tcagtcacca ccgcgcctgt caaaatctga ccacgtttgt tccagacacg caccagatcg
     6601 ccattcgcaa taccaaatcg agcagcatct tcggtgtgaa tagtgatcgg ttcacgatcg
     6661 gcaaccgcat attttttacg cagttccgca tagttaagct gactatgtaa acggtgtgcc
     6721 ggatgtgcgg tcagaagctg caactgcttc tcgtcggcgg tacccttcca ctcatcaggc
     6781 gcaagccagg ttggatgtgc cgggcaatcc ttatagccaa atttttccag cgttttggag
     6841 taaatctcaa ttttgccgct tggcgtaccc agcgcatttt tcaccggatc ggcgcggaaa
     6901 tcagcataac gaacgtactg ttcgttcttc tcgctgcggc gcatttcgat cagtttattt
     6961 tgctgccaga aggcattaaa catcggcata gtcacgcgtt gcgcacgagc acctttctga
     7021 gcggcatcat agaaaaattt cagccacgcc atttcatctt taccttcggt atagatctct
     7081 tttccgccag gtttcagtaa ttcagcaaga tcggcaaata cgtcaaaatc gttacgcgct
     7141 tcaaattgcg gagcaacagc ctgcttcatc ggcacaatat gctggttgct gtaatcaccg
     7201 gtcatcgtca agtcattgcg ctcaaacgat gtggtgatcg gtaatacgat atctgcatgt
     7261 ttagcagctg cggtccagta gcattcagaa acgacgatca tctccggttt ctgccatgct
     7321 ttgatcagac gattagtatc ctggtgatgg gtaaagttac cgccacccgc ccaccagatc
     7381 attttgatat tcggatacgt ttgttcttta ccgttatgct gatatttgcc accaggattt
     7441 tccagcgcat cgacgatacg ggcaacacga atcgccgtca ttccgccatc atcagcggcc
     7501 tcgctggcct gtccggcaat cgccgcagac atttccggca gcacgccgcc aacacgcgtc
     7561 ggattgccac cgttggagta atgataagag aaaccaaaac cgccgcccgg tgtaccaatc
     7621 tgccctaaca tcgccgccag cgtcaccagc atccagtgtt tctgttcacc atattgctgg
     7681 cgctgaattc cccagccagc catcagcatt gtgcgattcg ccgccatcag ttcagcgagt
     7741 ttgactatct gcgcttccgg tacgccagta atttccgccg cccaggccgc gctcttcgga
     7801 gtattatcgc tcttacctgt cagatactct tcaaactgcg gatacccggt ggtgtatttt
     7861 tcaaggaaca ctttatcgtg tttaccttgt gtcatcaggg tatgcgcaat ccctaacatc
     7921 agtgccacgt cggtgcccat attcggcgcg atccaggtgg cattatcacc aaagaactcg
     7981 atggtttcgg agcggatagg atcaatggca atcactggtt tgccagattt tttcagctga
     8041 tggaagtatt ccagcccttg ctcatcggta ctgctccagg caatttttaa ggtattcagc
     8101 gggttcatcc cccacagcac cacagcctgg ctgttttcca gaatcagcgg ccaggaggtc
     8161 tgctgttcat acacctctac agaaccgacc acatgcggca tgatcacctg tgctgccccg
     8221 gttgaataat cgccgctatg cccggaataa ccgcccgcca ggttcatata acgttgcagt
     8281 aaggtttgcg ctttatgcaa cacgccagaa gagcgccagc cgtatgatcc ggcaaaaata
     8341 gcagatggtc cgttagcttt gcgaatgcga tcgtgttgct catgaatcag ttttaatgct
     8401 tgctcccagc tcacctgtac ataagtatct tcgccacgac ctttcgccgg ttgcagtgga
     8461 ttatcgagat agctttttct taccatcgga tgctgaatac gtgccgtggt gtgtacctga
     8521 tccgccgccg tagactgtaa ggagttcggt atggttttcg ccagcgcgcc tgttgaagaa
     8581 acaatcttgc cgtccttcac ttctacgttc atcgctcccc aacgtcccgc ggtgaggatt
     8641 ttaccgccct tctcttctgc ccatgcgggg agcggtgctg ccgatgtcac caccagcgct
     8701 ccagcggcaa taccgctgtg tttaataaat tcacgtcttg ttaatgtcat aacttcctcc
     8761 ctgatcaacg aggatcactg tttctcggta atatctttgg cgttgtactg gaaataccgc
     8821 gttaaaatgt ccagttcgtt ttcgctcatg ctggttcgcg cacccattcc tttggcaatg
     8881 gacggccatg cattgacggt gtaatggtca gcggcaatag gcgcatggca accagcgcaa
     8941 taggtatcgt caagtttttc agcgtattgc catagcggtt tacggtccgc taatgcggga
     9001 tcggtaagcg caccctgtaa agacgcctga cgccattgat tgccgtattc gtcagcctgc
     9061 cattccccgt ttaccgtgag cgccttgata ccttcttcac ttaatgtggc tagcgccagc
     9121 cgttgacctg ccgccaggta gagcgtgttt tcactgccct gcatttgata accctgcaac
     9181 agaacgatcg gctgtttgcc actggcatca acgacggtga gatcggtacc aggattcacg
     9241 gtagccagct cgcctatgcg agaagttttg aaaggataaa tatgtgcgcc attagtcact
     9301 gaagtagcgg cctgactttc cagctcatgc gccgcgttgt catccatttt tatttctggc
     9361 ggaaaatggg caatgccttt atgacaatcg atacaggttt cgccgccctt ttgtgctttg
     9421 ttatgcattt tctgcgcaga ttcactttgc gaggcaatat ccatggcatc aaaagaatgg
     9481 caactacggc acgttgcaga gtcagttgct tttaattctt tccatactgt ttcggccatt
     9541 tcctggcgat gagtttcgaa cttatcgtca ctgtctattt tgccgctaac aaattcatga
     9601 taaatatctt tagatgcttt taatttagca aataaataat ccatccctga ctttggaata
     9661 tggcaatctg cacattccgc acgtatccct ttctggttcg aaaagtggac agttccctga
     9721 tattcctcaa aaggtttact catcgagtgg caggaaaggc aaaatgctgt atccgacgtt
     9781 ttatgtaagg ctttttgcgc cagcaataac ccgccgccac caaccacaac cgctatcagc
     9841 aaaaacaata acccaatgcg ttttttccct cgcatagatt cctctaaata aattccagaa
     9901 caccgaaatc attgtataat taaatacaca acgaattaac cgcttgcata actcggcaca
     9961 acactgcctg aaa
//
LOCUS       DAZFRJ010000160         9923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000160 DAZFRJ010000000
VERSION     DAZFRJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.162"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..564
                     /gene="osmC"
                     /locus_tag="ACHKUN_003664"
     CDS             133..564
                     /gene="osmC"
                     /locus_tag="ACHKUN_003664"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO6661856.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(620..1546)
                     /locus_tag="ACHKUN_003665"
     CDS             complement(620..1546)
                     /locus_tag="ACHKUN_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO6661857.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(1539..2525)
                     /locus_tag="ACHKUN_003666"
     CDS             complement(1539..2525)
                     /locus_tag="ACHKUN_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6661858.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2522..3418)
                     /gene="ddpC"
                     /locus_tag="ACHKUN_003667"
     CDS             complement(2522..3418)
                     /gene="ddpC"
                     /locus_tag="ACHKUN_003667"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO6661859.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3415..4437)
                     /locus_tag="ACHKUN_003668"
     CDS             complement(3415..4437)
                     /locus_tag="ACHKUN_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6661860.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4439..5989)
                     /locus_tag="ACHKUN_003669"
     CDS             complement(4439..5989)
                     /locus_tag="ACHKUN_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6661861.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(6003..6584)
                     /gene="ddpX"
                     /locus_tag="ACHKUN_003670"
     CDS             complement(6003..6584)
                     /gene="ddpX"
                     /locus_tag="ACHKUN_003670"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO6661862.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(6842..9241)
                     /gene="dosP"
                     /locus_tag="ACHKUN_003671"
                     /gene_synonym="pdeO"
     CDS             complement(6842..9241)
                     /gene="dosP"
                     /locus_tag="ACHKUN_003671"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO6661863.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(9266..>9923)
                     /gene="dosC"
                     /locus_tag="ACHKUN_003672"
     CDS             complement(9266..>9923)
                     /gene="dosC"
                     /locus_tag="ACHKUN_003672"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO6661864.1"
                     /translation="QDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLREL
                     FEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTW
                     GHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVE
                     KTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL
                     "
BASE COUNT         2442 a         2583 c         2502 g         2396 t
ORIGIN      
        1 tctattgtgg aaatttaatt taagtgcaga agtaatattt ctgccggatt tttattcgga
       61 atatcctgct tatcctcgtg ctgtttctca cgtagtctat aatttccttt ttaagcccac
      121 aggagagcaa caatgacaat ccataagaaa ggtcaggcac actgggaagg cgatatcaaa
      181 cgcgggaagg gaacagtatc caccgagagt ggtgtgctga accaacagcc gtatggattt
      241 aacacgcgtt ttgaaggcga aaaaggaact aaccctgaag aactgattgg cgcagcgcat
      301 gccgcatgtt tctcaatggc gctttcatta atgctggggg aagcgggatt tacgccaaca
      361 tcgattgata ccaccgccga tgtgtcgctg gataaagtgg atgccggttt tgcgattacg
      421 aaaatcgcac tgaagagtga agttgcggtg ccgggtattg atgcctctac ctttgacggc
      481 atcatccaga aagcaaaagc aggatgcccg gtttctcagg tactgaaagc ggaaattacg
      541 cttgattacc agttgaaatc gtaaagcatt gccggatgac gcgtcaggcg cgtgaatgcc
      601 tgatgcgttg ttagcatctc tacagcgccc gccagcaccg gatctcacgt ccgtcctccc
      661 ttattgttaa tgattgccgg acttcacatc cgtgggttgc cagagggcaa cgttcgtaga
      721 aaaaacagcc ttgcggcaac gtgcggtttc ccggcagatc cgttttacgt aatgcccatt
      781 cttcctccag cggtttatca atggcgggga gggaatccag caataatcgg gtatatggat
      841 gtgccggaac agtcaacacc tgttgcgctt cccctagttc cacaatctgc ccgagataca
      901 tcaccgccac ccgatcgctc atatgacgta tcaccgagac attgtgtgaa atcagcacat
      961 aagtcagccc gtgattttcc tgtagcgtta ccagtaaatt gaggatctgc gcctgcacgg
     1021 agatatccag cgccgaggtt ggctcatcaa gcacaatcac gtcaggctgc gaagagagtg
     1081 ctctggcaat ggcgatgcgt tgccgctgcc cgccggagaa cgcatgaggc aggcggtcga
     1141 gatactccgg acgaataccc acctgcacag ccagctcctc tgccaacgct cgccgctgtt
     1201 gttcactact acgcttagct atccagagcg gttctgtgat gatgcgccac accggtaagc
     1261 gcggattgag cgaagagagc gggtcctgaa acaccatctg cataatgcgt tgtgagccag
     1321 aacggatgta ctgcccgtgg ctcggttgca gcatgcccat taaaagctgt gcgagggtgc
     1381 ttttgccgca gcctgactcg ccgacgatcc ctaaggtttc accacggcgg atctgtaaat
     1441 caataccatt aatggcatga acatgttccg tagttttacc aagccagttt ttacgggccg
     1501 ggaaattgat atggacgtcg cgtaacgtta ataacgtgtc agacacttat gacctcctgc
     1561 tgcggatacc aacaggcgca gcgctggttg ttgtcaccac acgctgtcag cgccgggacg
     1621 ttttcacact gtgcacctgc cgcatagcaa cgatcgcgaa aagcgcagcc ttcaggcaaa
     1681 tgggtgaggt ttggtaccgt ccctggaatg gcgggtaata gctggcgtgg tactccatgt
     1741 tctggtgcgc attggagcaa accaatggta tacggatgcc ggggatgatg gataacatct
     1801 gccgtcacgc cgctttcaat tacgcttccg gcatacatca cgtaaacgct atcgcacagt
     1861 tgcgacacca cggccatatc atggctgatg aacagaaccg cagttccact ggcgcgggct
     1921 ttatgtttaa gcaaacgcag tacctgcaac tgcaccgtga cgtccagcgc cgtagtcggt
     1981 tcgtcggcaa taatcaattg cggctcgcag gagaatgcca gcgcaatcat tacccgctgg
     2041 cgcataccac ctgaaagctc aaacggatag cgcgacataa cttccacggc atccgggatt
     2101 tgcatctctt ccagcagggc aatcgcttta gctctggctt cccgacgact tattggttga
     2161 tgatggcgga tcacgtccat catctgaaga cctattcgaa gtgtcggatt gagggcggtc
     2221 atcggttcct gaaagatcat cgccactcgt gcgccgcgcc actgacgaag ctgtttttcc
     2281 cgagcgttaa gaacatcgtc tcccagcagt gaaatctgcc cccgatgtac gcaataactg
     2341 cccgtctgta gcagacgcat aatcagcatt gcggtgactg atttacctga gccggattct
     2401 cccaccagac cgacaatttc gccgcggtta atctgcaagg acacattgtt gagcgcgtga
     2461 acatcaccgt taaaaccggg gaaactcaaa tgcagctgtt gaatgtccag aacgggttgg
     2521 gtcatgactg ctttcctcct gctttcgggt caagcagatc gcgaatacca tcaccaaaga
     2581 gattaaaccc aacggcggta agcaaaatcg ctgcccccgg aaatgcgcaa taccaccact
     2641 gatcgagtac atagttgcga ccattcgcta ccatcgcgcc ccattcagca ctcggttgtt
     2701 gagcacccag gccaataaat cccaacgtgg cggccattaa aatcgcgcta ccgatatcca
     2761 gcgatgcctg cacgatcagc ggcggcaggg agttacgtaa aatatgccag ctgatcagat
     2821 gccaacgcga cgcaccaaag gttttcgccg cctgaacata ggtatactgg cgtacaacta
     2881 atgcttgccc ccgcgccagg cgcacataaa agggaattcg cacaatagca agcgccagca
     2941 tggcgttaaa taaactcggc ccgagagcgg cagccagtgc cattgtcagt accaacgaag
     3001 gaatcgacag cataatgtcc atgatgcgca taatgatggc gtctgcgcgt ccgccaagca
     3061 caccggatag acatccgagt agcgaaccaa tcatacccgc aatggcaacc acgactaatc
     3121 cggcgagaat tgattgctga ctgccgacca gtacgcggct gaacagatcg cgtcccactt
     3181 catcggtgcc aaaccagtgc gccgcagaag gcggcaaaag gcgggcggtt aaatcaatgg
     3241 cgttgggatc atgcgtcgcc agccacggtg aaaaaatcat catcaatagc attaatacaa
     3301 tgatgactgc gccggtcacg gtgagcggac tgcctttcag catccatacc agttttgcac
     3361 cgttaaatcg cgtttgtttt tgtgggcgta cggcggacgt ttcctcgctt agcatcattc
     3421 accacctcca cgtccgatac gcggatcaat ccacaaatag agcaaatcca ccaccaggtt
     3481 gaccagcaca taagcaaatg aaaccacgac ggcaaagccc atcactgccg ggaagtcgag
     3541 cgcctgtatt gatgttacta cccatgcacc cattccgggc caggcaaaaa cggtttcggt
     3601 gagcactgcg ccatacaaca aatcgcccag cgccaaaccc aatacggtaa tcgatgggat
     3661 caacgcattg ggtagcgcat aacataaaac gatataccag ccgggcaagc cgctggcccg
     3721 ggcggtacga atgtagtctt cactcaattg ttccagcatc gctgagcgga tctggcgagc
     3781 gacaattccc aggtgaacga acgccagcgt taatgccggt aagatgagat gttgcaacgc
     3841 attgaagaag acttcaccgt tgccttcaag cagcgcatcg agcagataaa agccggtaac
     3901 gtgcgttggt ggatccagcc agtcatcaag ccttccgccg ccgggaagaa tttgcagatg
     3961 accataaaac agcacaatga cgcccagccc aagccagaac gcaggtgtgg agattccggt
     4021 aatggccatt attcgcacca gatgatccag ccagcgattt cgccagactg cagagagtat
     4081 gcccagcggg atgccaatca ggagtgccag cagcagggca ccaaaagcca gctccagcgt
     4141 cgccgggaaa aatatacgca actcttccag caccggacgc ccggtacgaa tggatgttcc
     4201 caggtcacca tgaaacaggt cgctgacgta gcggtaaaac tgtacataca gtggctggtc
     4261 cagtcccagt tgctggcgaa tattttccac gatagcatcg ctggcgcggt cacccgccag
     4321 taaccgcgcc ggatcgccag ggatcagatg cgagataata aacgtaatca cgcagacgcc
     4381 cgccaccacg agcaccagcc cccagcagcg ttggcgtaaa atactccaga aggtcacgtt
     4441 atttactcat ggtattgata ttgaagacct gttccagcat gggattgaac acaaagcctt
     4501 tcacctcttt gttcatcgcc agttggtagt ttttctggaa caggtacaca taagcagcgt
     4561 catcaatgac gattttctgt gcctgctggt agtcccgggt acgctgcgtc tggtcggtgg
     4621 tcgcaagcgc attgcgcagt aacttatcga cctcactgtt ttcatagaac gagcggttac
     4681 ccggcagacc ttttttgtct gactcaaacc agtaattcat aaacatatac gggtcggcaa
     4741 aatccggact ccagttgcca atcgcaatgt cgtaatcacc tttacccact ctgtcgcgca
     4801 tggtggcgtt cgccagcttt tccagcttca cattgatacc cagcttgttg agactggatt
     4861 gtgtcgccaa agcaataggc tcccagttcg gatcgttatc ggagtagaga aacgtcaggc
     4921 tggtgggttt gctcgtcact ttatcccatt cggctttggc tttcgtttcg tcatggttgt
     4981 attgcattgc cgtcgcatcg tagccccaca tgccttccgg aatcgggccg cgcatctgtt
     5041 ttccgttacc actcagaatg ccgttaacca tgccctgata atcggtagac caggaaatgg
     5101 cccgacgtag atccgcctga ttaagaggcg ctttgctgtt attcagatac agataggtaa
     5161 cgcgcagtga cggatactct gccacattga ctttattttc ctgcttcagg gcgttgagtt
     5221 gatccaccgg cagcgcatcg gcaatgtcaa tgtcgccacg ggagagctgc aggcgacggg
     5281 aggcactttc accgataatt tttaccgata ctcgcttaaa gttcggttta ttgccggggt
     5341 aatgcggatt tggcaccaga actaattgct gacctttttg ccagcttttc agcataaatg
     5401 gtccggaacc ggcggtattt tgcgcgagga agccgcgagc atcatccgct gcatgttcct
     5461 ttaagaccgc cggattgata atggatgcac cgtcattcgc cagcgtgtag aggaacggtg
     5521 cgaatggttg gctaagggta aacttcaccg tatgttcgtc gggagcatca atctttaaat
     5581 ctttgggaaa tgcttctgct ggcccctggc cgatttttag tagccgctca aaagaaagtt
     5641 ttactgcttc ggcagtgaca ggtgtgccat cggcaaattt agcattatct ttcagggtga
     5701 acgtccactc tttttgatcg tcagacgctt tccagctact tgccagatcg ccttcaacgt
     5761 cggttgagcc tttatcaccg tccgttttgt actgaaccag ccgctgataa gacgggtagg
     5821 tcaccgtcca gtcgttatta tctattgtta ccgccgggtc gagggtttgt ggatcggcgg
     5881 ccttaccaat caccagcata tctttcggta cggcggcgtg cgaaaccggg aaatttgtgg
     5941 caaggacgag cgcgagcaat gtgggacgaa acgatatcga tctcttcatg acagtttcct
     6001 gattaactga cgtgctgtgt gccaggggat ataaaacaag agaattgatc agcgagcaga
     6061 gggtaactcg ctgcctgggg taattcgaag tgccaccatt cgctggagat accgacaaaa
     6121 ccgccaccag tcattatcgc attcagcaac agccgattgc gctgagcggc gggcgggacg
     6181 gaagggtgat aggcatggga gcgctcgtgc atttcatcga accctgcgcc catatcgagg
     6241 atgttcccgt gctcatcacg aagcgtcagg tcgatcgccg tgccacggct gtgattagaa
     6301 ccgaccgtca catcaacaac atattgcggg tctgggcagg cttgccacaa catcgcctgt
     6361 gcttgttgtg ggcgatacgc atcgtaaatc accagttgta agcctgacag ctgggcgata
     6421 ctgatacttt tcgccagcgc ggtaatcgca tccttgtgta gcagacaacg cgcttgctga
     6481 taaatagctt tacctgtgat gttatcagcg caggcgtatt tcaattcgat ctccagatca
     6541 gggaagatta cggctaaatc aaccagttcg gtggtatccg acataaacgg ttcctgtata
     6601 agacaaaaat tgctgcgctt tccgcttatg cagatctcat gccatgccgg gataagcgcc
     6661 agaatgctgg cttaaagtta tattttttaa cttttgatca acatttgtgc agcgtagtgc
     6721 agttttggtg caagagggga agttaaggaa ggaatctccc ggaatcgtag ctgaaatcac
     6781 agtatttaag tgacagtgtc acgttaaatg aaaacccgcg agtgcgggcg agaggaattt
     6841 gtcagatttt cagcggtaac acgctgctca tccagcctgg aatttcttcg gcgggtaggg
     6901 ggcgggaaaa gaaatatccc tgaataacgc gacagtggat cttgcgtagc atctcaaatt
     6961 gctctttggt ttcgacgcct tccgccacga cggttaaatt gaggctttgc ccaatgctgg
     7021 taatggcttc aagtaaggca aggatgcgtt tttcggtcag acaacgatcg acaaaacttt
     7081 tgtcaatttt gatttccgtt accggaagac tgactaagcg ggataatccg gaaaagcccg
     7141 taccaaaatc atctaccgat aagcccacgc ccatatcacg caggatctga atgcgcttaa
     7201 agatttcggt atcgtgttcc atcatcatgc tttccgtgat ttctaccgtc agctggtggc
     7261 cgtcaatacc ccaggcttgc attgcatcag acacctgatt aggcagctga ttactgcgaa
     7321 agtgcagcgc cgacaagttc acggataacg ccgggatatg aatattctgg ctacgccatt
     7381 ctgctaactg acggcaagct tccgcgatga cccagcgccc aatattttcg atttcaccaa
     7441 tctcttctgc gagaggaata aaccgtgaag ggggtacatg accatgctgg ggatcgtgcc
     7501 agcgagcaag ggcttcgatg ccgtacagtt cacccgtttc tgcgaagatt tgcggctggt
     7561 aaaccagttt cagttggtta ttgctaatcg cttctttcag cgctgcgcct aaaaccaaac
     7621 gctcttttac catttcgttc atcgccgggc tgaagaactg ccagccgtta ccgccattct
     7681 tgcgaatata atccattgca ttgtgagcag tggagagtag gtaatcgcgg tttttaccca
     7741 cgtcgtagct gatgccaata ctcaaggtaa gcgggaatgg tttatcgtca atcattatcg
     7801 gcttgctgac cacattccgt agctcatcgg cgatctgggt aatgttactg acgtcgtttt
     7861 ccaggctcac gaggacaaac tgcgtacctt cgatacgaca gagatactga tccggtttga
     7921 gtttttcacg aaagcgattg accacttcca gcaatgcctg atcggcccac gcatagccaa
     7981 ggctatcaat cacatcctga atatggtcaa caccgatgag atacaccacc ggagagacgg
     8041 ctttgtcgac caggtcatcg aggtaattgt gcaggttatt gcgatttggc agaccggtca
     8101 tcggatcaaa ttggatgagt tgttcaatat gctggcggct tttttcctgt tccagcgcca
     8161 gcgcggccat atgctgactg atatctgcca cgcgttcgat aaaggcgctg gtttctgctc
     8221 ctgacgaggt tttaatttgc aggatccccg caggcgcgcc atcacgctga cgaatggtcg
     8281 ctgaccagct ttgcgcattt tgaacttctg caccgtgcga agatgacgcc cagtgtatcg
     8341 gcatcccgtt gcgcagtgcg aacagcgaaa catgcgattc gttgagaaca gattcgatgt
     8401 tacgacaaat gatttccccc atttcatgaa atggcgggct gctgcacatg gcggcgagaa
     8461 tattgccttc aagctggcgg atttgccgtt cttcggtgat atccgagaaa gtcattacca
     8521 ggttctgcag atgcgcgagc acgtcataaa ccgggctgat agaggcttta atccagattt
     8581 tttcaccggt gcgcgtcaac agcagaaatt cgtcctgatc gcgggcggtt ttccatagca
     8641 actgttgtaa acgaatgcgg ttatcggcag ggaattcagg aatgttcagg agtgtatcgg
     8701 gctgcatacc gctggcttcg ctaatgcagt aaccaaacat ttcggtaaat gcgcgattgc
     8761 actgcacaat atggcgttcc ggatcgagga caatcaccgg tcggtcgaga tggtcaacgg
     8821 caataatcaa ttgtcgagtc tgttcttttt gcgccatttc tacgctggca tcccgtacca
     8881 gcgccaggta ataaactttc ccctcggcgc tcactttcga tagcgcaaaa cgggtccaga
     8941 ttttactgcc gtcttttttc tccagctgca gctcccgact catcccctcg acacgcgctt
     9001 taccgccttc acggttatga cgaatgtatt caggatgcgc aggacgcaaa tcccgcggaa
     9061 tcagcatatc aatgttattg ccaatgactt cttcacgttt gtacccccag agcttctctg
     9121 cggcggggtt gaaaaacatc acttcatcat tttcgttaat taacaccgca cccatcatat
     9181 tttgctcaag ggcggggaaa aaaatgccat cggcggcatt atccgcatcg gttagcttca
     9241 tgattacctc tgcatcctgg cgcatctaaa gactggcttt ccagagttca acacggtttc
     9301 tacctcgtct tttggcgata tacagagctt catcggctat ttgaatgagg cgctcatagt
     9361 caggatgacc attaaacatg gcggcaccga tggaaagtga gagggcaata tcctcgccgt
     9421 ttgcggcttt cagtttggtt ttctccaccc gactgcgaat acgttctgcg gtacgtaacg
     9481 tttcgttttc agaagcttca gtcaaaacaa tgataaattc atcgcccccg tagcgaaaaa
     9541 cataatcact actgcggacg ttgtcataaa aggcctgaga gactttacgc agaatttcat
     9601 cgccagtgtt atggccccac gtatcgttga tctctttgaa tttatcaacg tcaataatca
     9661 gcactgacag tggtgtaccg gtccggttgg catgggcaat ttcgcgtttg aagatagtcg
     9721 gcaggaaacg gcggttaagt aatttcgtca gtacatccat accgacttcg tggcgcgata
     9781 cttcttcaaa caattcacgc agcaaggtaa taatttgcga tacagtattt cttatctgta
     9841 ataaaaattt cacccgcaga cttctgttat tcaaattcct ggtgttacgc atggtttgat
     9901 tgaatatacc gtcgaaatcc tga
//
LOCUS       DAZFRJ010000161         9871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000161 DAZFRJ010000000
VERSION     DAZFRJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.163"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..609)
                     /gene="yncG"
                     /locus_tag="ACHKUN_003673"
     CDS             complement(<1..609)
                     /gene="yncG"
                     /locus_tag="ACHKUN_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO6661865.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNE"
     gene            875..2374
                     /gene="ansP"
                     /locus_tag="ACHKUN_003674"
     CDS             875..2374
                     /gene="ansP"
                     /locus_tag="ACHKUN_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO6661866.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2489..3550)
                     /gene="yncE"
                     /locus_tag="ACHKUN_003675"
     CDS             complement(2489..3550)
                     /gene="yncE"
                     /locus_tag="ACHKUN_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO6661867.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3792..5894
                     /gene="pqqU"
                     /locus_tag="ACHKUN_003676"
     CDS             3792..5894
                     /gene="pqqU"
                     /locus_tag="ACHKUN_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO6661868.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5930..6595)
                     /gene="mcbR"
                     /locus_tag="ACHKUN_003677"
     CDS             complement(5930..6595)
                     /gene="mcbR"
                     /locus_tag="ACHKUN_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO6661869.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6793..7830)
                     /gene="curA"
                     /locus_tag="ACHKUN_003678"
     CDS             complement(6793..7830)
                     /gene="curA"
                     /locus_tag="ACHKUN_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO6661870.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8011..8529
                     /gene="mddA"
                     /locus_tag="ACHKUN_003679"
     CDS             8011..8529
                     /gene="mddA"
                     /locus_tag="ACHKUN_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO6661871.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8526..8975
                     /gene="ydcZ"
                     /locus_tag="ACHKUN_003680"
     CDS             8526..8975
                     /gene="ydcZ"
                     /locus_tag="ACHKUN_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6661872.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8976..9209)
                     /gene="ydcY"
                     /locus_tag="ACHKUN_003681"
     CDS             complement(8976..9209)
                     /gene="ydcY"
                     /locus_tag="ACHKUN_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO6661873.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9295..9468)
                     /gene="ortT"
                     /locus_tag="ACHKUN_003682"
     CDS             complement(9295..9468)
                     /gene="ortT"
                     /locus_tag="ACHKUN_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO6661874.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9663..9758
                     /gene="yncL"
                     /locus_tag="ACHKUN_003683"
     CDS             9663..9758
                     /gene="yncL"
                     /locus_tag="ACHKUN_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO6661875.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2363 a         2418 c         2425 g         2665 t
ORIGIN      
        1 ttcattcctt tttaatactt cttgtaattt cggtaactga catacagcat cagcgatcgc
       61 gctgatattc tgcgcattat cctgaaacca ttcatgcccc ggcccccatg tgcgcatggt
      121 gcagagataa cagtccacca gcgttagttg ctcaccaaag gcataaggtt cagcggtcaa
      181 ttgtgaattc agccagatat aaagtgattt gcgatattca attacattct tctttaactg
      241 ttcaggtgcg tcagatgccc aacgctcagg gtaatcggcg aaagtgaatg ttggatagac
      301 attagcaacc agccagacca atagccgttg aaactgctga cgttcggcgc gcccaaccgg
      361 tggcgcaagg tccgggcgac gatcgagaac cataaatgca atcgccgctg tctccgtcat
      421 aatctcgtca ttttccagcg ccagtgtcgg aacctgacac aacgggttca gggttttcaa
      481 taactcgcgt gaagctccct cgtggtcaaa accactgaca tcaacgaact gataaggaat
      541 atcagccagt gttagcatca gctcactgat tgtagagccc catccgggta cgccatacac
      601 cttaatcata tgccctcaca atcttttaaa gattaagtgt aggacagtac acaggttaac
      661 cagcaaaaag atttgtcatt ttttcgtcag ttgtattatt ccccttcagg attgtaaggg
      721 gatgttttgc atcgtttatc gataacgggt aggatgctgc gccgatagtt ttatacccat
      781 aaagaataat ggtgataact atcatcgcca ggatgaataa acattgttca tggcaactta
      841 tatgactttt tcattaaagc aatcagggag agcaatgagt aaacacgaca ccgacacttc
      901 agatcaacac gccgcgaaac gccgctggct taatgcccac gaagaggggt atcacaaagc
      961 gatgggcaat cgccaggtac agatgatcgc cattggcggc gcgattggca ccggcttgtt
     1021 tttaggtgca ggagcccgac tgcaaatggc gggtccagca ttggcactgg tttatttaat
     1081 ttgtggcttg ttttcgtttt ttattctgcg tgcattgggt gagctggtgc tacaccgccc
     1141 ttccagtggc agttttgtct cttatgcccg tgagtttttg ggtgagaaag ccgcttatgt
     1201 tgctggctgg atgtacttca tcaactgggc gatgacgggg attgttgata ttaccgccgt
     1261 cgctctgtat atgcattact ggggtgcgtt tggcggcgtg ccgcagtggg tctttgcgct
     1321 cgctgcactt accatcgttg gcaccatgaa tatgatcggt gtgaaatggt ttgcggagat
     1381 ggagttctgg tttgcgctta ttaaagtgct cgccattgtg acctttttgg tcgtgggtac
     1441 agtgttcctc ggtagtggtc agccgctgga tggcaacacc actggctttc atttaattac
     1501 cgataatggc ggcttcttcc cccacggttt gctgcctgct ctggtgttga ttcagggcgt
     1561 agtgtttgct tttgcctcca ttgaaatggt gggtacagct gccggagaat gtaaagatcc
     1621 gcagaccatg gtgcctaaag cgattaacag cgtgatttgg cgtattggcc tgttttacgt
     1681 cggctccgtg gtgttgctgg ttatgctatt gccgtggagc gcgtatcagg cggggcaaag
     1741 tccgttcgtg acgtttttct ctaaactggg tgtgccatat atcggcagca ttatgaacat
     1801 tgtggtgctg accgctgccc tctccagcct gaactcaggt ctgtactgca ccggacgtat
     1861 tctgcgctca atggcgatgg gcggttccgc accgagtttt atggcgaaaa tgagtcgtca
     1921 gcatgtgccg tatgccggga ttctggcgac actagttgtg tatgtcgtcg gcgtattcct
     1981 caactatctg gtgccgtcgc gcgtatttga gattgtgttg aacttcgcgt cgctgggaat
     2041 catcgcttca tgggcgttta tcatcgtgtg ccagatgcgc ctgcgtaaag caattaaaca
     2101 aggcaaagca gcggatgtca gttttaaact gcctggcgcg cccttcactt cctggctgac
     2161 attactgttt ttactgagtg tcctggtgct gatggcgttc gattacccga acgggactta
     2221 tactatcgcg gcgctgccga ttatcggtat tctgctggtt ataggctggt ttggtgtgcg
     2281 caaacgcgtt gctgaaattc acagcactgc gccagtcgtc gaggaagatg aagaaaaaca
     2341 ggaaattgtg tttaagcctg aaacggcgag ctaattgcaa aattgcccgg ctacgtgccg
     2401 ggcaaattta gattactgac gaaggtgtgc cgcatgcggc gtgataaaag tcaggcaagt
     2461 ctctccgttt tcaccctcgc cacatctatt acagcgcaat acgaatcaca tcgtccggct
     2521 gggtagcttc ctgctgttta gtggattttt gtttcacact gacatacagc gttttgccat
     2581 cggcagacag cgccaggctg tttggatgag tcggcgtatc gaacgttttc accactttat
     2641 agcttttcgc gtcaatcaca ctgactttac ctgcctgacg atgcgttacg taggcttcat
     2701 tacgcgctgg gttaaacagc acagccagtg attctggtgc cgcaaccttc gccagaatat
     2761 tgccattacg ggtatcgacc actaacactt ctgcggcttt agaatcggtg ataaatgcac
     2821 gctgcctggc ggtatcaagg ctaatgttga taaagaagtg ctctttgccg tcatccagca
     2881 gctttttacg gctgaggatt ttattgtcgg cggtgtcgat ggtaatcaat tcgccgtcag
     2941 cgttagtggt gtaaagacgt ttgcctttgc tatccagcgc cagaccggta ctcattttac
     3001 cggtgttctg gatggcggtt ttcagtttga tattctcgcc atcaacgacc caaatcacgc
     3061 tctctttacc aataccactg atgtaaacgg tgttcgtggc atcatcagct accagctcac
     3121 gcggttgcag cgggcgcacc tcttccgtgc gcttacgatc atccagcacc agacggcctt
     3181 tcacctcgcc cgttttggca tctatcgccg tgaccgcgct gtttacggtg ttaccaaacc
     3241 acaacgtctg agtcgtgtta ttgatggtgg caccaaacgg cttgagatcg ttatggatcg
     3301 cctgcgtcac ttccagagtg accggatcaa gacgataaac cacgccacct ttatccagtt
     3361 tgcggctttg cgaagtggcg agccacagtg cgttttcttg ctggctataa gccatttcgt
     3421 aggcaccttt acctaccgct ttacgcagca tttcttctgc ggcctgcgta ctgaatgatg
     3481 aagcaacaag caatgaacct aacagtaatg aaccacgcag gcgcgatgaa aacagatgac
     3541 gtaaatgcat gacgactccc tttgatgaac cgatattttt gctgcttcac aatgcttcgc
     3601 taagcgtaat catcgttacg ctcttgttat aaatgggaat ggtaatcatt attttctcaa
     3661 atgtggagca tttcttgccg ttacgactac aattttctca attacttaac gtatcgtgcc
     3721 gtttcgtttt ccataaattt acaatttcat taccataacg gcgttttccc attcttaacc
     3781 tggctttttt catgaagatt ttttccgtcc gacagaccgt tttgcccgca ctgcttgtcc
     3841 tttcccccgt tgtttttgcc gctgatgaac agactatgat tgtcagtgcc gcaccgcagg
     3901 tggtttcaga actggatacc ccagcagcag taagcgtggt ggatggcgag gagatgcgcc
     3961 tggcaacacc gcgcattaac ttgtccgaat cactgaccgg cgtgcctggt ttgcaggtac
     4021 aaaaccggca gaactatgcg caagatttac agctgtcgat tcgcggattt ggctcccgct
     4081 ccacttacgg tattcgcggt attcgcctgt atgtggacgg tattcccgcc accatgcccg
     4141 acgggcaagg gcaaacatcc aacatcgatt taagcagtgt gcaaaatgtg gaagtgctgc
     4201 gtggcccctt ctctgccctg tatggcaacg cgtctggtgg ggtaatgaat gtcaccaccc
     4261 agaccggaca acagccacca accattgaag ccagtagtta ctacggcagt tttggcagct
     4321 ggcgctatgg gctgaaagca acgggcgcaa cgggagacgg cacacagcct ggcgatgtcg
     4381 attacaccgt ctcaaccacg cgttttacga cccacggcta tcgtgaccat agtggcgcac
     4441 agaaaaattt agctaatgcc aaactgggcg tacgcattga tgaagccagc aaattaagtc
     4501 tgattttcaa tagtgtggat atcaaagcag atgacccagg tgggctaacc aaagcagaat
     4561 ggaaggctaa tccacaacaa gcgcctcgtg cagaacagta cgacacgcga aaaaccatca
     4621 agcaaactca ggctgggttg cgctatgagc gtagcctgag cgcgcaagat gatatgagtg
     4681 tgatgatgta tgccggagag cgagaaacga cccagtacca gtcaatcccg atggcccccc
     4741 aacttaaccc gtcacatgcg ggcggcgtga ttaccctgca acgccattac caggggatag
     4801 acagccgctg gacacaccgt ggtgaactgg gcgttccggt cacgttcact accggcctga
     4861 actacgaaaa catgagtgaa aaccgcaagg gctacaataa cttccgcctg aatagcggca
     4921 tgccggagta cgggcaaaaa ggtgagttgc gtcgcgacga acgcaatctg atgtggaaca
     4981 tcgatcccta tttacagacg cagtggcagc tgagcgaaaa actgtcgctg gatgctggcg
     5041 tgcgctacag ctccgtgtgg tttgattcca acgaccatta cgttactccg ggtaacggcg
     5101 atgacagcgg tgatgccagt tatcataaat ggctacctgc cggttcgtta aaatatgcaa
     5161 tgaccgatgc ctggaatatc tatctggcag ccgggcgagg ttttgaaacg ccgacgatta
     5221 atgagctgtc ttatcgtgct gatgggcaaa gcggtatgaa ctttggttta aaaccatcca
     5281 ccaacgatac aattgagatc ggcagtaaaa cgcgtattgg tgatgggctg cttagtctcg
     5341 cattgtttca gaccgacact gatgatgaaa ttgttgtcga tagcagtagc ggtgggcgta
     5401 cgacttacaa aaatgccgga aagacccgtc gtcaaggcgc tgaactggca tgggatcaac
     5461 gtttcgcagg agattttcgc gtaaacgcgt cctggacctg gcttgatgcg acctatcgca
     5521 gcaatgtttg caatgaacag gattgtaacg gtaatcggat gccagggatc gcccgtaata
     5581 tgggctttgc gtcgataggt tatgtaccgg aagatggttg gtatgcaggc acggaagcgc
     5641 gttatatggg cgatattatg gcagatgatg aaaatacggc aaaagcgccg tcttatactc
     5701 tcgtcggctt attcaccggg tataaataca attaccacaa tttaactgtg gatttatttg
     5761 gtcgtgtcga taatttattc gataaagaat acgttggttc tgtcattgtc aatgagtcaa
     5821 acgggcgata ttacgaacct tcgcccggac gaaattatgg tgtcggcatg aatattgcgt
     5881 ggagatttga gtaattacct tgatgcccgg tatttgccgg gcatttactt taacgattgt
     5941 attgctggta taaaatagca atattttgtt gcataatttc agcaagacaa tgtctgcatc
     6001 cctctttgtc ttttgctttt aacgcagcga gtaacagatg atagttttct atatgctccc
     6061 gtaattccgc tggattaatc gcttcataga gataatgtaa accaggcccc atcctgaccc
     6121 acagttgctc aatcatctca cacaggatgg gcatatttga gcgatgataa atcgctaagc
     6181 gaaatagcct gtttacatta atgatttgtt ccatgtcgcc cttttcttgc gcctgctgta
     6241 atttctcgag caattcctgg agttccgcca ggtcttgcgg ggtgaggttt tcaacagcca
     6301 gagcaactgc cattaattcc agttcgtagc ggatccgatt gatttcgtct aattgacgtt
     6361 tcctcacttc cggaactgta aatgcttgtg caggtgcgac agaaagcgca ttcaccgaaa
     6421 ccagacgtaa taatgcttca cgcacaggtg taatactcat acctaattgc tccgccagat
     6481 ttttagtaat caggcgtgcg ccaggtttta atgcgccaat actaagctga tgtttcaggt
     6541 cgttctcaac ctgcagagtc aaactgacat gtttcatttt tcccattcca ggcattttaa
     6601 ttccttcgcg tgttttctgt ccaaccctgc ctgcacggca gaaggagttc acctggtgta
     6661 ttaaagtgaa taatcataaa ccacccacct ttgttgttat tagcagcaga acaatgacgt
     6721 ggttagttaa ttaaagctga aaataagctt tttgcctgga tttttatggc ggcttacgcc
     6781 gccgttgaaa ctttaatcat cacccgcgac gcggatcacc actttgccga agtttttacc
     6841 cttcagcagg ccgataaacg tctgtggcgc attctctaaa ccatcagtaa tatcttcgtg
     6901 gtagtggatt ttatcctctt tcacccattg ccccatctcc ttctgaaact catggatgcg
     6961 gtgaccataa tcctgagcga taataaaccc ttgcaagcga atacgttttt tcagcaccgt
     7021 tgccatcaac aggggtaaac gatccggacc gggtggtagc tctgtagcgt tatagctgct
     7081 cactaagcca caaacgggaa tgcgcgcaga tgtattaagt aatggtagca ctgcatcaaa
     7141 taccttaccg cccacgtttt cataatagat atcaatacct tttgggcacg ctttcaccag
     7201 ttgttcggca aaatcatccg cgtggtgatc aagacaaaca tcgaagccca gcacctcgat
     7261 agcatggcgg catttttccg cgccaccggc aacccccacc accctgcaac ctttaagttt
     7321 gccgatttgc cccaccgttg ctcccacagg acctgtcgcc gcagctacca ccaacgtttc
     7381 gccctcttta ggctgaccga tatccagcag ccccatataa gcggtaaagc ctggcatccc
     7441 cagcacaccc agcgaccacg atggattttg cggatgatcg ccaagtttca ccagatcatc
     7501 accactggat atgtcatagt cctgccatcc actgtagccc agcacccagt cgccaggctg
     7561 ataatcagga tgattcgact ccacaacacg gctcaccgtg ccgccgacca tcacgccgcc
     7621 aatatcaaca ggcggtgaat aagatggctc atcgctcata cgtccacgca tatacgggtc
     7681 cagggacaaa taaactgtgc gcagtaacac ctgtccttca cccggtgtgg cgacatcatc
     7741 ttcttcaaga cggaaattct ccggaaccgg tgcgccatgt ggacgcgagg ccagaaccca
     7801 acgtcgatta cgctgctttt gttgcctcat aatgctctcc tgtgcttttg gcatgaatcc
     7861 atcaatagta gcaggctcac gggattggtt ttgtttgcag ccgtcacatc ccctctgcaa
     7921 cacgcaataa tttcatcgag gaatccgttg cttcgggctg ttttcgatag tattgataac
     7981 atcatcatca ttattttatc gaggccagac atgtccatcc gttttgcccg caaagccgat
     8041 tgtgctgcca ttgcggaaat ttataaccac gccgtgttgt atacggcggc tatctggaat
     8101 gaccaaacgg ttgatgcaga taaccgcatt gcctggtttg aagcgcggac catagcaggt
     8161 tatccggtgc tggtgagtga agaagatggc gtagtgacgg gatatgcctc gtttggcgac
     8221 tggcgtagtt tcgatggttt tcgccatacc gtggaacatt cggtttatgt ccatcccgat
     8281 catcagggca aaggtctggg gcgtaaattg ttaagccgat tgattgatga agcgcgggat
     8341 tgcgggaagc atgtcatggt cgccgggatc gaatcgcaaa atcaggcctc gctgcatctg
     8401 caccagtcgc tgggatttgt cgtcaccgca caaatgccgc aggtgggcac taaatttggg
     8461 cgctggctgg atctgacatt tatgcagttg caactcgacg aacgcactga accggacgcg
     8521 attggatgaa tcagtcgctc acccttgctt ttttgattgc cgccggaatt ggtctggtag
     8581 tccagaacac gctgatggta cgcatcaccc agacctcctc taccattctc atcgccatgt
     8641 tgctgaactc actggtgggg attgtgctct ttgtttctat tttatggttt aaacaaggca
     8701 tggcggggtt tggcgaactg gtgtccagcg tgcgctggtg gacacttata cccggcttgc
     8761 tgggatcgtt ttttgtcttc gccagtatca gcggatatca gaatgtggga gcggcgacaa
     8821 ctattgcagt actggtcgcc agccagctaa ttggcggtct gatgctggat atattccgca
     8881 gtcatggcgt accactacgg gcgttgttcg gcccaatttg tggcgcaatt ttacttgtgg
     8941 ttggcgcctg gttggtagcc agacgctcat tttaatcaga ggatggtgcc gcctttggtt
     9001 aactgttcgt ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg agcaatagcg
     9061 gtgcgcaaac gctgctgctg ggtgtagcga tcttcccgac ttaactccgc gtcatcgctc
     9121 agggcgatta ataattcgtt catatgggca atgacgctct cttcaatcgc ggcatccacg
     9181 cgcgcgatga cttcatccag atgcgacata ctttctcctt aaccacttgt gaaatttatc
     9241 gattcatcca aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta agatttaaaa
     9301 cagtgaaaac aacagcgcca ccgggaaact catcggccat gttgccccca ccagaaaggc
     9361 gcttaagaaa cggatgcgtt tcttatcgtg agaaagaaac caggtgataa gaaatgcgat
     9421 tgctgccata accgcataaa aaacaagcat atgttgatag agagacatgt acgggcgtcc
     9481 agccagatga ataaaacgcg cgcaaatatg cgtcattttt tgacgtgcat caagtttttt
     9541 caccagtttg agaaaaaatc atcaaacagc aacttgccct tatccatctt cagaatgagt
     9601 attattgcgg attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa
     9661 aaatgaatgt ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt
     9721 ttactcttat ctctatgaga tttggctggt ttatttgatg tagtgatttt gcaagcagca
     9781 cgtactgtca gagaaatcaa ctctctgaca gtacgcaaga tcttctcata ctctccaggt
     9841 ttacaacatt atccttaata tttacttctt t
//
LOCUS       DAZFRJ010000162         9865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000162 DAZFRJ010000000
VERSION     DAZFRJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.164"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHKUN_003684"
     CDS             complement(<1..153)
                     /locus_tag="ACHKUN_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661876.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNY"
     gene            231..1325
                     /gene="ddlA"
                     /locus_tag="ACHKUN_003685"
     CDS             231..1325
                     /gene="ddlA"
                     /locus_tag="ACHKUN_003685"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6661877.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1349..1561)
                     /gene="yaiZ"
                     /locus_tag="ACHKUN_003686"
     CDS             complement(1349..1561)
                     /gene="yaiZ"
                     /locus_tag="ACHKUN_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO6661878.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1821..2129
                     /gene="yaiY"
                     /locus_tag="ACHKUN_003687"
     CDS             1821..2129
                     /gene="yaiY"
                     /locus_tag="ACHKUN_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO6661879.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2188..3282)
                     /gene="yaiW"
                     /locus_tag="ACHKUN_003688"
     CDS             complement(2188..3282)
                     /gene="yaiW"
                     /locus_tag="ACHKUN_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO6661880.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3295..4515)
                     /gene="sbmA"
                     /locus_tag="ACHKUN_003689"
     CDS             complement(3295..4515)
                     /gene="sbmA"
                     /locus_tag="ACHKUN_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO6661881.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4867..6024
                     /gene="ampH"
                     /locus_tag="ACHKUN_003690"
     CDS             4867..6024
                     /gene="ampH"
                     /locus_tag="ACHKUN_003690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO6661882.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6025..6648)
                     /gene="iprA"
                     /locus_tag="ACHKUN_003691"
     CDS             complement(6025..6648)
                     /gene="iprA"
                     /locus_tag="ACHKUN_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO6661883.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6736..9433)
                     /locus_tag="ACHKUN_003692"
                     /pseudo
     CDS             complement(6736..9433)
                     /locus_tag="ACHKUN_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9463..>9865)
                     /locus_tag="ACHKUN_003693"
     CDS             complement(9463..>9865)
                     /locus_tag="ACHKUN_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6661884.1"
                     /translation="SRAVIGWSMSPRMTAQLACDALQMALWWRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
BASE COUNT         2565 a         2479 c         2316 g         2505 t
ORIGIN      
        1 ataatttaga gaaaaaaact caccgcatgg ccatttaagc caggaataaa ttagcttccc
       61 gttcatcatt tttgtgatct tgcgcacatc tttgagataa cgcactttct tcaccagtac
      121 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
      181 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
      241 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
      301 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
      361 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
      421 ctgcccatat tgcgttacgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
      481 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
      541 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
      601 atttaccgtt cgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtta
      661 ccaaacgtct gctgcgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
      721 ccaatcgtca caacattagt tttgccgaag tggagtctaa actggggtta ccactgtttg
      781 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
      841 acgcaattgc cgtcgatctg gcgttcgaat tcgatcataa agtgatcgtt gagcaaggga
      901 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caacccgcaa gccagcacct
      961 gtggcgagat cgtactcacc agcgacttct atgcctacga caccaagtac attgacgaag
     1021 atggcgcgaa agtggtagtt ccggcagcca ttgcgccaga aatcaacgat aagatccggg
     1081 cgattgccgt tcaggcttat caaacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     1141 ttttaacccc agaaaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     1201 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     1261 cacgtttgat tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa
     1321 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     1381 gacgaataat cagccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     1441 aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata
     1501 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     1561 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     1621 tagacaacat tttcgtaatt gttaataaat ttaacttaag gttgccattt tgtgactcac
     1681 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     1741 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     1801 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     1861 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     1921 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     1981 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     2041 ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat
     2101 ggctgcactg gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc
     2161 cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     2221 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     2281 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     2341 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     2401 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     2461 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     2521 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     2581 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     2641 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     2701 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     2761 accggtgcgc acaggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     2821 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     2881 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     2941 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     3001 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt
     3061 ttccagcgtc ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc
     3121 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     3181 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     3241 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     3301 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     3361 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     3421 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     3481 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcaa cctgcaaata
     3541 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     3601 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     3661 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     3721 cagtttgatc cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc
     3781 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     3841 cgttaccagc accggcagaa aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     3901 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
     3961 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     4021 gcggaacaca tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     4081 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     4141 cgaacttagc gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac
     4201 cccgacttcc accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca
     4261 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     4321 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     4381 gatctgcccg gaagcgccgg tgatacgcgc cacccagtca cccccaccgg cttgccagaa
     4441 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa
     4501 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     4561 taaccgcatg accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     4621 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     4681 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     4741 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     4801 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     4861 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     4921 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
     4981 gccgatcata ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt
     5041 aaccagcgcg tctttcgcag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
     5101 ctggattcgg tcgtgcgtat tgcttccctc accaagctaa tgaccagtga aatgctggtg
     5161 aaattgctcg accaggggac cgtgaagtta aacgatccgc taagtaaata tgctccgcca
     5221 ggcgcacgcg tgccaaccta caatgggacg cccattacgc tggtcaatct ggcaacccat
     5281 accagcgcgc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     5341 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     5401 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg ctgatgcact ggcgaatgcg
     5461 tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa
     5521 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc agagcgcggt
     5581 gccagtccat gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     5641 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     5701 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     5761 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggtcccgaaa
     5821 gagggtcgtc cggggattat tcagaagaca ggaggtggtg gcggtttcat tacctatatg
     5881 gcgatgatcc cgcagaagaa tattggcgcg tttgtggttg tcactcgttc gccgttgacg
     5941 cgctttaaaa atatgagcga tggcatcaat gacctggtga ccgagctaag cgggaataaa
     6001 ccgctggtta tccccgcgtc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     6061 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     6121 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
     6181 caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt
     6241 atgcccaatg agctgcacgt cgcgtaattc cagaattcta ttttgccagg ctaaccagta
     6301 aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag
     6361 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
     6421 accatcagcc agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc
     6481 tctacgcaga gagataactc cctctaaaat aacgaaagta tcttcgttat tgacatcact
     6541 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
     6601 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
     6661 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagaatttct
     6721 tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc
     6781 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
     6841 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
     6901 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
     6961 agtcagagcc tggtcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
     7021 acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
     7081 accagactgg aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag
     7141 tttgaagtcg taaccggctt tcaaaccgaa gccccaagcg tcggagttgg tgctaccgtc
     7201 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
     7261 gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg
     7321 gctgtcttga tccacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc
     7381 cgcaccgacg atccacttag cgttgttacc gtcaattttg gtatcaacac cgaccatgat
     7441 gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa
     7501 gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt
     7561 accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc
     7621 catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc
     7681 agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt
     7741 gttgacgtcg tttacgtaga taatttcttt atctttgtaa tcagcgatag cgccagcgcc
     7801 tgttgcgttg tcgatacgaa ctttgtagtt accggttgca gcaacaacgt tgtcaacaga
     7861 gctgttatcc agttcgtcgc cgttgttcat gtcaccgtta ccgttgatag tcagatgacc
     7921 atcagagttc atagcaacaa taccgtagcc gtagttagac ttggaagtat cccaggtacg
     7981 gtcgttcacc aaatcagcgt tcagaacata atcgctgctg tggatatcga atacaccaga
     8041 cactacgttg ccgttatggt cagcgatgtt gccgctggtc agttccaaag tatcagtctg
     8101 gaacggatta tcatcgaact ggtctacatt aatatccagt aaaccaccgt tagtcacagt
     8161 tattgtattt gctaacagcg acggagtgta ataaccatta tagatacttg aattgtcatc
     8221 actagacaac gctacagtac tgtagctatc aactgtcaga tgatcagttc caacataacc
     8281 ttcatctcca atgttcaaag aagaaccatt ggtcagagcg atgctatcag aaatcaagct
     8341 agattcagac acattaacct gagagccatt attcacagct aaagtatcaa tgtttgaagc
     8401 gttaacggta ttccattcag aaccattatt cagagtgacg ttaaacacac cgctctggaa
     8461 tacttgatca tcaatcgtac gaccccaagc atttgcttct gttacaacgc ccggatacag
     8521 gctattacct gtaacatcat accagtcagt cgaagcagta gattcatcat caacatttgc
     8581 tgatgtagta gctgagccta cccacttaga accattatcc agagtgatat tcagttcatc
     8641 aacattaagt gagtcatcag cccagccacc attggtgtcc aagtacgcca tcggcattac
     8701 tgtcatgacc ttgaggatag aagtttgcgt tgaaagtact tgcgaaaaca acatcaccca
     8761 gcagagtaga attgctgaaa taaacattgt ttttcatgga gtaatttgaa gccgcactat
     8821 cactgaaata cagagctaca tcattagctt cgccgttacc agagtaatca ctaggttccg
     8881 cagagttacc atagaaacca ctgtcttcca gagtagttac agaacctgaa gtcactgtgg
     8941 aatcggtaac tgagatattg ttatcccaag tatgagattc agtagtgtaa gtattgttta
     9001 cggtattacc ctgagtcagg ctgataccag caacgcggga gttgttagaa atattgatgt
     9061 tactttcgac atcaagattc actgccagcc caaggccaac accaccgttt gcaaacaaac
     9121 cggcgtattc ttcaccttca ccagctgggg tatggaaggt ttcgttagta tatttggtaa
     9181 cgtcaccgtc cagatatgaa tcagtgaagt ataaaccttc ataatcgtca tcaattgtag
     9241 aattagcaat atttaaagtg aatacatcac catcatgcca attatcatca attacacgat
     9301 cagtaccgta ttcagagaac tgatagacat aatgaccatc actgctttca cccataggca
     9361 gcattgaagt gattgaacca tgaatcaggg agttgctgat gttcaactgg tttgttgttc
     9421 catcagtaaa gttgatccta cccacgtaat atggacacag gcctaagcga ggttcttgtt
     9481 ttcaaattgt tccggactga ggccgccaca caaactgtgc cgccgccacc gattgtaatc
     9541 acattcgata taattaaata ccgttgcccg cattatttcc cggctgataa agtgttctcc
     9601 atggatacat tccactttca gcgaatgaaa gaagctttcc acgcaggcat tatcgtagca
     9661 gcaacctttt gcgctcatac ttccacgcag attatgccgc ttcagttgcg cctgataatc
     9721 tgctgaacag tactggcctc cacggtccgt gtgaacgata acgttccggg gcctcttacg
     9781 ccaccacagc gccatctgca gggcatcgca ggccagttgc gccgtcatgc gtggcgacat
     9841 tgaccagcca ataacggcac gtgac
//
LOCUS       DAZFRJ010000163         9851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000163 DAZFRJ010000000
VERSION     DAZFRJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.165"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..344
                     /gene="bdm"
                     /locus_tag="ACHKUN_003694"
     CDS             129..344
                     /gene="bdm"
                     /locus_tag="ACHKUN_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO6661885.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            446..583
                     /gene="sra"
                     /locus_tag="ACHKUN_003695"
     CDS             446..583
                     /gene="sra"
                     /locus_tag="ACHKUN_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO6661886.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            740..2437
                     /gene="maeA"
                     /locus_tag="ACHKUN_003696"
     CDS             740..2437
                     /gene="maeA"
                     /locus_tag="ACHKUN_003696"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6661887.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2571..3581
                     /gene="adhP"
                     /locus_tag="ACHKUN_003697"
     CDS             2571..3581
                     /gene="adhP"
                     /locus_tag="ACHKUN_003697"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO6661888.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3728..4012
                     /locus_tag="ACHKUN_003698"
     CDS             3728..4012
                     /locus_tag="ACHKUN_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6661889.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4063..4716)
                     /gene="fdnI"
                     /locus_tag="ACHKUN_003699"
     CDS             complement(4063..4716)
                     /gene="fdnI"
                     /locus_tag="ACHKUN_003699"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO6661890.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4709..5593)
                     /gene="fdnH"
                     /locus_tag="ACHKUN_003700"
     CDS             complement(4709..5593)
                     /gene="fdnH"
                     /locus_tag="ACHKUN_003700"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO6661891.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5606..8653)
                     /gene="fdnG"
                     /locus_tag="ACHKUN_003701"
     CDS             complement(5606..8653)
                     /gene="fdnG"
                     /locus_tag="ACHKUN_003701"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8066..8068),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6661892.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8885..9766
                     /gene="yddG"
                     /locus_tag="ACHKUN_003702"
     CDS             8885..9766
                     /gene="yddG"
                     /locus_tag="ACHKUN_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO6661893.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2394 a         2582 c         2488 g         2387 t
ORIGIN      
        1 aaaatataaa aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
       61 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
      121 gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
      181 atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca
      241 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
      301 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgatc
      361 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
      421 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
      481 actggaccat aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt
      541 ggtaaataat ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
      601 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctctata
      661 cttggggccg acgccctggc ggtaaagcaa agacgataaa agcgtgccag ggatggatat
      721 tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc
      781 ttacgctggc cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat
      841 ggaagaacgc cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga
      901 agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca
      961 catctacctg cgtaacatcc aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa
     1021 tcatcttgat gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg
     1081 tttttctgag atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accggcacaa
     1141 tatggacgat attctgcaaa acgtgccgaa ccataatatt aaagtgattg tggtgactga
     1201 cggtgaacgt attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg
     1261 taaactgtcg ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt
     1321 gctggatgtc ggaacgaaca accaacagct gcttaacgat ccgctgtata tgggctggcg
     1381 taatccacgt atcactgacg atgaatacta tgaattcgtt gatgaattta tccaggctgt
     1441 gaaacaacgc tggccggacg tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat
     1501 gccgttactt aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac
     1561 tgcggcagta acagtcggca cactgatcgc agccagccgc gcggcaggtg gtcagttaag
     1621 cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat
     1681 catcgcccag acccagcgcg aaggattaag cgaggaagca gcgcggcaga aagtctttat
     1741 ggtcgatcgc tttggcctgc tgacggacaa gatgccgaac ctgctacctt tccagaccaa
     1801 actggtgcag aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgttgtcact
     1861 gctggatgtg gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg
     1921 gctgtttacg gaagagatca ttcgtgagat gcataaacac tgtccgcgtc cgatcgtgat
     1981 gccgctgtct aacccgacgt cacgcgtgga agccacaccg caggacatta tcgcctggac
     2041 cgaaggtaac gcgctggtcg ccactggcag cccgtttaat ccagtggtat ggaaagataa
     2101 aatctaccct atcgcccagt gtaacaacgc ctttattttc ccaggcatcg ggctgggtgt
     2161 tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg atgtcagcaa gtgaaacgct
     2221 tgctcagtat tcgccgctgg tgctgaacgg cgaaggtctg gtactaccgg aactgaaaga
     2281 tattcagaaa gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg
     2341 cgtggcggtg aaaacgtctg ccgaagctct gcaacaagcc attgacgata atttctggca
     2401 agccgaatac cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct
     2461 accgggctat ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa
     2521 tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg
     2581 cagttgttac gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac
     2641 atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta
     2701 agaatggcga ttttggtgac aaaactgggg taattctggg ccatgaaggc atcggtgtgg
     2761 tggcagaagt gggtccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt
     2821 ggttctacga aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc
     2881 gttcagttaa aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg
     2941 tcgccgatta cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta
     3001 cctgtgcggg ggtcaccacc tacaaagccg ttaaactgtc aaaaattcgt cccggtcagt
     3061 ggattgctat ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg
     3121 tctttaacgc caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg
     3181 aaatgggtgc agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg
     3241 agaaaaccgg tggcgctcac gctgcggtgg taacagcagt tgctaaagct gcgtttaact
     3301 cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt
     3361 ctatgagcct ggatatccca cgccttgtgc tggatggcat tgaagtggtc ggttcgctgg
     3421 tcggcacgcg ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc
     3481 cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag
     3541 gcaaaatccg tggccgtatg gtgattgatt tccgccgtta agaggccttt gctgcgagtg
     3601 ccagtcctgg tgtcgcagca tcgcactctc tccgacatta aatggctaaa tggattattg
     3661 ttccttccta cgcaacaata atgatagtgt tcctgatgaa ggaacactac tgagagtaaa
     3721 gacactgatg aaaatggcca atcatccccg cccgggggac attattcagg aatcactgga
     3781 cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgctc cctcaacggc
     3841 aagccgattg ctgaccggaa aagcagcttt gacaccagaa atggcgatta aactttccgt
     3901 ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga gtctggcaga
     3961 ggcagaaaaa acggtggatg tgtcgcgact acgccgtctg gttacgcaat aaaaaaatgg
     4021 cgcttcttaa agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
     4081 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
     4141 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc
     4201 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
     4261 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
     4321 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
     4381 cccggcgttg tacttaccga catccgccac tttatgctca ttgcctttca atacttcgac
     4441 aatgttcaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
     4501 aaacataaac atcagtgcga cgaaaatcgc aatgccgaaa aacgggtgca aaatgcgtcc
     4561 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
     4621 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
     4681 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
     4741 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa
     4801 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
     4861 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
     4921 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg
     4981 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
     5041 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
     5101 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
     5161 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
     5221 tcccgttagc gtactgaatg attgcaccag cagacgggca cgccttcagg cagccgggat
     5281 cttcacagtg catacagccg tctttacgga tcagccactc cagcttgccg ttctgttcgg
     5341 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
     5401 ccccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
     5461 aggctttaca gccgatacag gtggaaacgt cgataagttt tgcgacttct gctttgtaat
     5521 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atgtcctgcg
     5581 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
     5641 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac
     5701 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
     5761 gaccatttac attcagtggt ttcagacgac gcgttaccac agccaccgcg cggataaagc
     5821 cacgcttgct tgagacagtg acacgatcgc cattattaat gcctttcgcc gccgccagcg
     5881 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaat gcgtgcttgg
     5941 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
     6001 ttttacccat ccgcagtgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt
     6061 tcggatgcag cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg
     6121 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa
     6181 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc aggaatatcg ttacccgtcc
     6241 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
     6301 ccgccgaagc acggttgtac agcacgcgac ggttgagcgg ccacgcccag gcccatccca
     6361 gcgtattccc cagaccggac gggtcggagt tatcgcggtt agccatctgg ttgccctgct
     6421 ctgtccagct accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat
     6481 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga
     6541 gatcttccag cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
     6601 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcggcct
     6661 ggtacagctc gcgcaggtga tggtagatac ccgccagaat ttcaccgtcg ttacgcgctt
     6721 cgcccggcgc atcctgacct ttccagtgcc actgcagcca gcgaccggag ttagcaatag
     6781 aaccatcttc ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
     6841 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggttgaggtt tcagtcacca
     6901 gcggatcgat aaccaccatg tacttcagct tgcttaggca gctcaccact ttgtttttgt
     6961 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
     7021 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
     7081 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
     7141 agaacttcgg atagttgctc cagtagttca cctgaccagc cagcgtcgct ttcggcgtgt
     7201 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
     7261 gcaggctggt agagagcaga cctaagtcag tcagaccctg aatgttggag tgaccacgca
     7321 atgcattcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
     7381 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
     7441 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
     7501 cttttggcgt accacagatg ttttcaacaa cgtccggcgt gtagcgggaa acgtgcgctt
     7561 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt
     7621 tttcatcgaa ctgatagttc caggacgatt tatcgtattg ccgtttttca gcgtcgtagc
     7681 cgctgaacag accgtcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt
     7741 aatgcttaac gtattcggcg ttgattttgt cgttttcgat caggtagcgc aaaacgccag
     7801 acaggaacgt aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg
     7861 tacgcgtaaa acgaggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
     7921 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
     7981 cgttagcgtt tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag
     8041 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
     8101 cgagggagcg ggcaaatttc tgtgtcagca tcccggtttc gttgctggca ccggaggcac
     8161 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
     8221 agttagcgtc acggtcagct ttcatcagct ttgcaatacg ggagaatgct tcttcccagc
     8281 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
     8341 cgctgttgac gtaatccagc aaaccggccc cttttgggca cagcgcacca cggcttaccg
     8401 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac
     8461 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
     8521 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
     8581 ccaatgccgc taccgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc
     8641 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
     8701 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
     8761 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
     8821 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
     8881 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
     8941 gatggtagga ttgattcgcg gtgtcagcga ggggctcggc ccggtcggcg gcgcagctgc
     9001 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
     9061 gattccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
     9121 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
     9181 ctatctgtgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
     9241 ctggctgatt gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg
     9301 tgagaatggg ttacattatg atgaaatcat caataatatc accaccagcc cattgagtta
     9361 tttcctggcg tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata
     9421 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt
     9481 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
     9541 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatactgca
     9601 tggcaatgtc accataatgg cggtaggctc gtattttacg cccgtacttt cctcagcgct
     9661 tgcagccgtg ctgctcagcg ccccgctgtc gttctcattc tggcaaggcg cgctaatggt
     9721 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt
     9781 ctacggcccg gcagttaatc agataaattc cgacaatatt aaaaaataaa tttcaaatac
     9841 cagtaattca c
//
LOCUS       DAZFRJ010000164         9771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000164 DAZFRJ010000000
VERSION     DAZFRJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.166"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..553)
                     /locus_tag="ACHKUN_003703"
     CDS             complement(<1..553)
                     /locus_tag="ACHKUN_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO6661894.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKL"
     gene            complement(550..2238)
                     /locus_tag="ACHKUN_003704"
     CDS             complement(550..2238)
                     /locus_tag="ACHKUN_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO6661895.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2387..5014)
                     /gene="gyrA"
                     /locus_tag="ACHKUN_003705"
     CDS             complement(2387..5014)
                     /gene="gyrA"
                     /locus_tag="ACHKUN_003705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO6661896.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5161..5883
                     /gene="ubiG"
                     /locus_tag="ACHKUN_003706"
     CDS             5161..5883
                     /gene="ubiG"
                     /locus_tag="ACHKUN_003706"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO6661897.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(6012..9746)
                     /gene="yfaL"
                     /locus_tag="ACHKUN_003707"
     CDS             complement(6012..9746)
                     /gene="yfaL"
                     /locus_tag="ACHKUN_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO6661898.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2269 a         2723 c         2365 g         2414 t
ORIGIN      
        1 acaacttcca tgtcataagt tgttgcagac tgactgcgcg cattccgttg tcagttttcg
       61 tggcgcttcc ggtgtggtag atgacgtaac gccccatcca gaccattaag tgctgggcat
      121 cgccctgatc gaaaaaaatc atatcgccag gcagcgcctg gtttatgtcc tggccaataa
      181 actggctgtt gtactgaatc aaattaatcg cggtcacgta ggggccggtt ttcccgttcc
      241 cctgattcca gttttgcgcc agttgacgct gtccaggtgt tagcgtcatc tctggcggca
      301 aatactggct agagaaaccg ttacttttta gccatttact atcgtgaact ttcagcgtct
      361 cgttcgccgc aaatcgcacc aggcccgcac aatcctgctg ataccagcgt ggacttggcc
      421 cctggcggag ctgttcttgt gcaatgcgta caaaccaggc gcgaaacagc ccggattgtt
      481 cgacattcag catttcgcta tgggcaacaa cacaacacag ccagcaaatc agcgccagca
      541 gcccgtgcct catagcggct gccaggttat gggtagccac tgccaggcgg caccgggttc
      601 catctgggcc agcttcatga cataacgcgg ttgttgagat aaagcgtcca gcttcggcat
      661 taataaagtt tgtgcggcgt tataaaaaac cggttcgaga ttcttcggca gactggtcaa
      721 cgtttcgtta cgcagcagtt tcgccacgcc ttgtggattg atatagagcg gaacgatgcc
      781 atcagtgggt attacatcca ccattgccgg gcggttttta ttcagtgttt gcagggcgtt
      841 attaaccagc gtgtcatcga gggagaaaag cagcgttttg ttttgcatcg ccagcgacac
      901 gcggaaaaaa taatccgaca ttaattgatc gggttgcgcc gcctgcgctt tcggatactg
      961 accgtatcgg gaactcactt cgcgacgcca gatttgcgct tcgccctgct gagtctggct
     1021 tatcggcaaa acaccttctg gcgctttgct ttcgtgcgca ccaatatttt gcgtaaacag
     1081 tttccctggc aattgcgcct gttcggcagt gccatcaaac tgaccgacaa acagcggggt
     1141 ttgcaatttt gagtctgcat accagcacag ccccgcggca ccgtctaacg ccccattcaa
     1201 cttgtcgttt tcctgactga tgtgcgaaag catctcttcg gcaataccgt gtgaatacgg
     1261 caccaccaca cagaagctgg ccccggcagg catactgttc cagaccggcg taaaatcgaa
     1321 actggtatct acgctggcgg attcatcatt taacgccaca aaactgtgcc agccgtcgtt
     1381 acccatttcg aagcgtacgc cagcaaaaga aggcattaac cgctggtagc caaaacccag
     1441 caacctggcg ctgaccacga tgcgctggcg tactggcgtt ttttcagcag tacgctcttc
     1501 caggccaaag cttgcttgcc agcgtttttt gccgctcaac aaatcacctg cgatcgccgt
     1561 ggcttcggta tcctgctgat catctttaaa caacatatcc gtgctggaaa acaccagcat
     1621 cttgtcctga taagtcgcga acatcagggc gttattgccg ttatagcgca actgataaac
     1681 aggaacagtt tcactattta tcttgatgct actgatttcc gttttgctta actggctgtc
     1741 gctggtagcg gcaaacaaca atggctccag cagtttgctt aaaccactgc gctggatcag
     1801 caccatataa tgtgaaagat ggccttgttt atcgtgccac agcgctgcct gcgcgggctg
     1861 atctaagagt gacgaaaaga gcttatcttt cagcgtgaga tcgtgttcat agacaatacg
     1921 acgaatgctg ccttcaatgc ccagacgatc ggcatgattc tgataataga aaacgaaatc
     1981 ttcgctcaga acatcgtgga gaaacggaat ggtgaggaga tctttgggaa gctggctcag
     2041 agagtcgctg tcgagaaaga ggtccggctc attgagatcg atttgcagat tgttgtgcac
     2101 caccagcggc gacaacgttt tttctggccc actgccagca tattgcaacg cccagacgcc
     2161 agcggaaagc agtgctattg cgccaaaacc tacaagacca tagaaccgcc agcctttcgc
     2221 ctttttttca ccactcattg ccacattcct tgtgtatagc cagccatttt ttacgggcac
     2281 agccaaactt taccgtgccc taatacgaca aaagcccaga ctttgcagcc tggacttttc
     2341 aattcaaaca agggagatag ctcccttttg gcatgaagaa gtaaaattat tcttcttctg
     2401 gctcgtcgtc aacgtccact tccggagcga tttcatcgtc cccttccgcg gcactgccat
     2461 cgatggtatc cagatcttcc tcgtcaaccg gttcagcaac acgttgcaga cccactacgt
     2521 tttcatcttc cgcagtacgg atgaggatca cgccctgggt gttacggcct acgatgctga
     2581 tttccgaaac gcgagtacgt accagcgtac cggcatcggt gatcatcatg atctggtcgc
     2641 agtcatctac ctgtaccgcg ccaaccacta aaccattacg ttcagtaacc ttaatggaga
     2701 taaccccttt cgtcgcacgc gacttggttg ggtattccgc cactgcggta cgtttaccgt
     2761 aaccgttttg cgttgcggtg aggattgcgc catcgccacg aggcacgatc agagagacga
     2821 ctttatcgcc ttcacctaag cgaataccgc gaacaccggt ggtgttgcag cccatcgcac
     2881 ggacagaaga ctctttaaag cgcaccactt taccttcagc ggagaacagc attacttcgt
     2941 cttcgccgct ggtcaggtca acgccgatca gctcatcgcc gtcaaccagt ttgatcgcca
     3001 ctttaccggc ggtacgcaga cggttgaact cggtgaggac agttttcttc acggtaccgt
     3061 tagcggtcgc catgaagact ttcacgcctt cttcaaactc ggtcactggc aggatcgcag
     3121 tgatacgttc gtcctgctcc agcggcagca ggttgacgat cggacgaccg cgcgcgccac
     3181 gagtggcttc cggcaactga taaactttca tcgaatagac gcgaccacgg ctggagaagc
     3241 acagaatatg gtcgtgagtg ttcgccacca gcagtcggtc gataaagtct tcttctttaa
     3301 tacgtgcggc agatttacct ttcccgccac gacgctgcgc ttcgtattca gaaagcggct
     3361 gatacttaac gtagccctgg tgagagagcg tcactaccac atcttcctga gtgatcagat
     3421 cttccaggtt gatgtctgcg ctgttggcgg tgatttcagt acgacgtttg tcaccgaact
     3481 gttcacgaac cagctccagc tcttcacgga tcacttccat caaacgatcg gcgctaccaa
     3541 gaatacgcaa cagttccgcg atctgatcca gcagctcttt gtattcgtcg agcagttttt
     3601 cgtgctcaag gccggtcagt ttctgcaaac gcagatccag aatcgcctga gcttgctgtt
     3661 cggtcaggta gtacagacca tcacgcacgc cgaactctgg ctccagccat tccggacgcg
     3721 cagcatcgtc gccagcacgt tcgagcatcg cggcaacgtt gcccagctgc cacggattag
     3781 caaccagcgc agttttcgct tctgcaggcg tcggcgcatg acggatcagt tcgatgatcg
     3841 ggtcgatgtt cgccagcgcc acggctaatg cttcaaggat atgagcacga tcgcgagctt
     3901 tacgcagttc gaaaatagta cgacgggtca ccacttcacg gcggtgacga acaaacgccg
     3961 cgatgatgtc tttcaggttc atgatcttcg gctgaccatg gtgcaatgcc accatgttga
     4021 taccgaaaga aacctgcaac tgggtctggg agtagaggtt gttgagcaca acttcaccga
     4081 ccgcatcgcg tttcacttca atcacgatgc gcataccgtc tttgtcagac tcgtcacgca
     4141 gcgcgctgat gccttccacg cgtttttctt ttaccagttc cgcaatcttc tcgatcaggc
     4201 gcgctttgtt tacctgatac ggaatttcgt ggacgataat ggtttcacga ccggttttgg
     4261 cgtcaacttc cacttctgcg cgagcgcgga tatacacctt gccgcgaccg gtacggtaag
     4321 cttcttcaat accgcgacga ccgttaatga ttgccgccgt cgggaagtcc ggccccggga
     4381 tgtgttccat cagcccttca atgctgatgt cttcatcatc gatatacgcc agacaaccgt
     4441 tgatgacttc cgtcaggttg tgcggcggga tgttggttgc catacctacg gcgataccgg
     4501 aagaaccgtt caccagcagg ttaggaattt tggttggcat gacgtcggga attttttccg
     4561 tgccgtcata gttatcaacg aaatcgaccg tctctttttc gagatcggcc atcagttcat
     4621 gggcaatttt cgccagacgg atttccgtat aacgcattgc cgccgcagag tcgccgtcga
     4681 tggaaccgaa gttaccctga ccgtctacca gcatgtaacg cagcgagaat ggctgcgcca
     4741 tacggacgat cgtgtcataa accgccgagt caccatgggg atggtattta ccgattacgt
     4801 caccaacgac acgggcagat tttttatagg ctttgttcca gtcattgcct agtacgttca
     4861 tggcgtaaag tacgcgacgg tgtaccggct tcaggccatc tcggacatct ggcagcgcac
     4921 ggccaacaat gaccgacatc gcataatcca gataggagct cttcagctct tcctcaatgt
     4981 tgaccggtgt aatttctctc gcaaggtcgc tcatctaacc gctatccctc tactgtatcc
     5041 cggattcaaa ggtcgcaaat tataacacag ccgcgcagtt tgaggtaaac ctatacgctt
     5101 tattcacatc caatgcctga tatactcgtt tgtcttgcca attacggagt agaagtgcca
     5161 atgaatgccg aaaaatcgcc ggaaaaccat aacgtagacc acgaagagat cgctaaattt
     5221 gaagccgtcg cctcccgctg gtgggatctt gaaggtgagt tcaaaccgtt gcaccgcatt
     5281 aacccgctgc gtctgggcta tattgccgag cgtgctggcg gtttatttgg caaaaaggtg
     5341 ctcgatgtcg gttgtggcgg cggcattctg gccgagagta tggcgcgcga aggcgcgacg
     5401 gtgaccggtc tggatatggg ctttgagcca ttgcaggtgg caaaactgca cgcgctggaa
     5461 agcggcattc aggtggatta tgtgcaggaa accgtggaag agcacgcggc aaaacatgcc
     5521 gggcagtatg atgtggtgac ctgcatggag atgctggagc acgtccccga tccgcagtca
     5581 gtggtcagag cctgtgcgca actggtgaaa ccaggcggcg atgtcttttt ctcgacgctt
     5641 aaccgcaacg gcaagtcatg gctgatggcg gtggttggcg cggaatatat tttgcgcatg
     5701 gtgcccaaag gcacgcatga tgtgaagaag tttattaaac cggcagaact gcttggctgg
     5761 gtagatcaga caagccttaa agagcgacat ataaccgggc tacattacaa cccgctcact
     5821 aatactttta aactcggccc cggcgtggat gtgaactata tgctgcacac gcagaataag
     5881 tgaggttgtt gtttggccga gccaatgcct gatgcgacgc ttgccgcgtc ttatcagtat
     5941 ctacaaatgc tccccgtagg ccggataagg cgtttacgcc gcatccggca accgtgccga
     6001 ctagacagtg attaccattt caccgtcatc gacaaaaacc ctgccgtctg ggcaaaatca
     6061 tcactccctt tctgccacgc cacgctgccg cgcagggaca ctcgctgact gatattgccc
     6121 gtgactccca cttttatttc accccgtcgc ttcaccgcat cgtcactgat agtgctgcca
     6181 tcttcttcaa tttccgtcgc atggggatcg tgataataat tcagatctaa tgttggtgtg
     6241 acaccaaccg cggtacgcca ttcgctgtgt aaacccagcc gcgtctgaat atcatccccc
     6301 tgcgattgtg acacgcgcgc atggttagcg gcggtaaaat catcctgctg cacgccctga
     6361 taaatcacct gcgcctgcgg ttcaatcacc acaccacgcc ccggtaacca ctgatacccg
     6421 gcttccagcg aggcgataat ccccgacgag tggtaatgat ctgtgccatc gtcttgttcg
     6481 gaaacatcat tgttaaacca cgcgtattgc agccagctat ccagccaggc cccctgcttc
     6541 tgattaccgt gctgatacca gcttgaggtc agcccaacgg cataaccgtg gttctggtta
     6601 tcggcgcgag ttccggtcat attcgagcgg ctctcgccct gattatcact gtagccacca
     6661 accgccccaa gcatccactc gccatcatcg ccccagcgcc cgctaaacag gtcaccgcta
     6721 agctgcaccg tagaagtgtc ttcatgctga gccagttgcc ctgccgccgt gtaatgataa
     6781 cgtccgccga taacacgtaa attcagcgtc tgaccattgc cacctgcatg atcgcgtcgc
     6841 tccatcataa acgcctgatt tgccgcccgc agattattga gataaccgcc aactttggca
     6901 ttcagcaccg gctggtaagc ggggattgga tcgggtgttg ggtcaggatc gggtgttggg
     6961 tcagggtctg gatcaggtgg caatggtggc gttacttctt gcgatcgcag ataccagtcg
     7021 ttgttatctt ccaccagcgt gtagtcatat gctcccatat tgacgtagcc gctgcctgcc
     7081 agactgaact tcgcattgtt ttgaaactgc gtgggatcgg ctgcgaaatc aaccacttta
     7141 atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac agttgtgttg
     7201 ccagcagtat taccgttcaa caccaattga tcgcttaccg agtcatcgcc gtttaattcg
     7261 ctatcgagca gtagcgtacc gccgccggta taatcaccgt ttaccgtcag cgtatcgcca
     7321 gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagattgcc attaaccgtc
     7381 acgtcggaaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc acctgtcaac
     7441 gtcacgccat cggtggcatt caccagcgtc ccatcgccgt taaacagtga ggcattaagg
     7501 gcgacagaag tatcctgccc ggtcaggcgc aaaaccgtac catcgctgat gtcgatagtg
     7561 ccgctggaag tagcatcaat agattgaata tcctgatccg cgccagtaac gaacgtcgcc
     7621 ccatcaccga cccacagtga cgaagcataa ggaaggatat ccgcgacatc gcctttcagg
     7681 gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag ctccagcgta
     7741 cccgcccccg ttttagtcaa tgtgctaccc tcatcctgat gctgcccgct gctgtcagcc
     7801 atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc gtcggcgcgc
     7861 atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt ggcagtaatc
     7921 tccatcacgc tttcttgttg cttatcggta aagataacct ggcggttata gtgggtatct
     7981 ccaagctgtg agttgtcgct cacctccagc ataccggagg cgatttgtgt tgtgcccaaa
     8041 taactattat tgtttgccag acttacctgc ccgtcgcccg catcgaccac cacgtagacg
     8101 ccatctttcc cgtcaccgcc gatatgcata tcgtttccac ctgcaagatc taccacatcc
     8161 tgactgccga taaccgttcc gctgctgccg ctgacttcaa ggccatcgtt gtatgatgtg
     8221 ccactctgcc aggtggagaa atcagagaga tcgagcacac cgccgtttaa cacgatcgac
     8281 tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga aagcaccgca
     8341 ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccccga cagcacgtag
     8401 ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tggtgccagc aaaactgcct
     8461 tgattaacag taacattgcc accagcatcg atatttaaag tgccattctg aaagcccgtc
     8521 aatgagtgca caaaggtttg ttgggtggag ccaacgttta gttctgcctg attctggtac
     8581 ttatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg gcaatgcgta
     8641 tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt ttcaatctgc
     8701 atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga acctgttttg
     8761 gtgatcaacc cggtaccagc aatagagtca acagctccgt cattctctgt attgccaata
     8821 accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag atacataaag
     8881 ccacccgccg cagtggaagg accatctcca tagcctgctg cgctgttgtt ctcatccact
     8941 aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag atagggagcc
     9001 gtcgcgctat tggtatatat cgccccgcca taaccttcgg cagtgttatt tgtaaaggcg
     9061 gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt atcggtaaca
     9121 tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg gttgttatca
     9181 aaaataacat cacttaaata aacgtcatta ttaatggtat aaattgcgcc acctttgcca
     9241 tcattagcga tgttattgcg aaacatggcg ttagtgacac gtaaatcgac gacaccagta
     9301 tcattagtac cagaagaata gattgcgcca ccatagccgc ctgcgacgtt accagaaaaa
     9361 ataacatcag taagattcag cgttgagttt tctttagcaa atatggcccc gccgttatta
     9421 tattctcctg taacggtgtt attggcaaac agagtcattc cagtttcatt ttctggtaat
     9481 agtgaaaatt ccgctccttg ttgcaaaaat acggctcctc cgctggcgtt attggtcata
     9541 tccgaaaaaa ccagccattg cccatcggcg acactccagt cctgcgtaat gcctgaaagg
     9601 ctttgcctgc tggcgtgaca actcgctttg atatcatatc cctggcataa atcaatggcc
     9661 gccgcgaaac cgttagcaga gaaaagagat gcaatcattg acgggagtag agataaatac
     9721 tccttgcgta gaaagataat ccgcatattc atctaaccat cattttatat a
//
LOCUS       DAZFRJ010000165         9756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000165 DAZFRJ010000000
VERSION     DAZFRJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.167"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..1067)
                     /gene="ompT"
                     /locus_tag="ACHKUN_003708"
     CDS             complement(114..1067)
                     /gene="ompT"
                     /locus_tag="ACHKUN_003708"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO6661899.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            complement(1580..2341)
                     /gene="envY"
                     /locus_tag="ACHKUN_003709"
     CDS             complement(1580..2341)
                     /gene="envY"
                     /locus_tag="ACHKUN_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO6661900.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(2524..3414)
                     /locus_tag="ACHKUN_003710"
     CDS             complement(2524..3414)
                     /locus_tag="ACHKUN_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO6661901.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(3415..6387)
                     /gene="nfrA"
                     /locus_tag="ACHKUN_003711"
     CDS             complement(3415..6387)
                     /gene="nfrA"
                     /locus_tag="ACHKUN_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO6661902.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(6374..8611)
                     /gene="nrfB"
                     /locus_tag="ACHKUN_003712"
     CDS             complement(6374..8611)
                     /gene="nrfB"
                     /locus_tag="ACHKUN_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO6661903.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(8880..>9756)
                     /locus_tag="ACHKUN_003713"
     CDS             complement(8880..>9756)
                     /locus_tag="ACHKUN_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO6661904.1"
                     /translation="ECFINWMRDCHSSNDKDVIAIDGKTLRHSYDKSRRRGAIHVISA
                     FSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGG
                     DYLFAVKGNQGRLNKAFEGKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDEL
                     IDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHV
                     ENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAM
                     DRNYLASVLAGSGLS"
BASE COUNT         2310 a         2427 c         2430 g         2589 t
ORIGIN      
        1 gttatgacat aggttaaatg cttacaaaat tagtggatat tgcctacttg taactgtaaa
       61 caaattcccc ggggttatac aataccaccg gggagaaaat ctggttaact tcgttaaaag
      121 gtgtacttaa gaccagcagt agtgatgaag ttatagtttt ctatgcctgc accatttttg
      181 ctgtagtctg aagtgttatc attgtgatca taaagtgaag tattaccttt tttattcgta
      241 acccgattcc atgcgccttc aacataaact tttgcgttag gtgttacgta ataacctgca
      301 ttgactgcaa cagaatagta attttggtct ttgactttac tgcgataagt gattcttttt
      361 cctgggtcat agtgctcatc gttatcagat gcttccaccc agccgctgta tttaaatgtg
      421 ccacctagct caaaatcttc ataacgataa cttccagtca agccaatgta gggcatttta
      481 aaacgttgtt tgtagccgat tgctctttct ccattcggga aggagccgat atcatctctg
      541 aatccctcct cagaactgta gatataggaa ccacctctgg ctgtaaagct ataacggctt
      601 tcctgatatc cggccatgag tcccaggcgg taattgggtt cgttgaggag ccagcctttg
      661 atattcagat caaattcgtt ggcataattg agttgtgtat cagggtgcct actttcatct
      721 gtccaggttc cggggttact ggaatccatc cagtcctgat cgaccatatt gccacctcgg
      781 ctgccgagag tagtccagcc cgcagcccca atagatatct ggggcatcaa atcccaatta
      841 attgcacctt taataattgc agcgttattg aatttccagt cgagttgact gacttttcgg
      901 cctccttctt cagctagata aacgcgctct tttgtttttc cgctcagagt tccaagacta
      961 atgtccgcat ttatgttgtc aggagtaaac gatatagtct cggtagaagc aaaagagctg
     1021 atcgcaatag gggttgtcag aactattccc agaagtttcg cccgcataaa agttctccat
     1081 tcaatcgttt taatgattga atatgtattt tttatatcta acttaatgag tcaatcacat
     1141 attgctccac tgtttatatt ttgtttagta ttgaatgatt atcacaatgc gctatctgtt
     1201 tttggtttaa ttatctgtta ttgttttatg ttgcggtttt actgtgtggt tttttatgct
     1261 tttgtggtgt ttttatctat ttaagcgcca tgcctttaaa ggcatataag cgaaaatagc
     1321 atgaggttta tcctcaattg ctatgttttt tagtacaaaa aagagggaca aaactgagac
     1381 acataaggcc tcacaatggc ttgcaaggct ttacatgttt tgaggtagtg ggacgtgtga
     1441 gcgcagagat ggcgcggtaa tttgttgact taaaatgtcg ttctaggaac ttctaagtcg
     1501 tgggccgcag gttcgaatcc tgcagggcgc gccatttctt cttcatttat gcccgtctta
     1561 tccgtttccg ctttgccctt caccacatca ctttttgtcg ctgtttggcg agataattca
     1621 acggtgtcag gccgtaaaac gccttaaaaa cagagataaa gtacgacgtg ctgctatagc
     1681 cacataattg cgccacctga gtgatatttt tgttatccat caataacatc tgtacggcgt
     1741 aacgcatacg acactctgtg acaatctggc tatagctggt attttcgttt tttaatttct
     1801 ttttgagtag gctggggctt aaacatagcg aactggcgac aattcgcaga ttccagtaat
     1861 gctgaatatc gctttgaata atgcggcaga cgctgtcgcg gacgctgctg cgtaagatat
     1921 acatcagtag ggctataaac cgcgattgct caagaaagtt agacaatacg gtaaaaagta
     1981 atgcgcgcgt caactccgct tcgcagggtt tgctgggttg ctgcttgctg tggttggcgg
     2041 ctagccggaa cacttccggt gtacggctat gacaagcgat aaccggggtt gccagccgcg
     2101 accagggctt tacacaggtt aagttcttat ttaaaaacaa cagataatcg ttgatgatgt
     2161 tacggttgat gtgggggatt aatacgtgat ttaacgttga gagttcaatg acgttattgt
     2221 tgcacgcgaa ggcggccagg tagtttttcg gaagggtaaa cgtagcatgg ttattgacgc
     2281 ttacctctac ctctttttcg gtcaggatca ccacgcaagg ttcactgctg ctcaattgca
     2341 tttcgcactc ctcagatatc agaaactccg ctcaaaggat ctatgcttcc tgcatgagtg
     2401 atcggcccgt tcgccgataa cgatcttctt tctttagcac gctttttagc aattaatctt
     2461 gatggaattc tgacgagagc gaaagaggta agccaggtcg tacccgactt acctggagga
     2521 gatttaatac tcgagaatgc cgtgcgcgac gggcaaatag cgcagagaga aatagagagt
     2581 gtctttaccg caagaggaac gtttcgctaa cagcgaggcg atctctgcat ccggtttcgg
     2641 tttcgctgta aaggttttgc ctttgccggc gacaaacagt tcataaacaa tgccgctggc
     2701 aggggcggga ctttggcaat cggcgctggc ctgtagataa cgttcacgct gttcagcggt
     2761 aagtttatcc acgccgctgc catcctgtac ccagacatta acgccggttg ctttaacttg
     2821 ttccagcagt tggcgatagc catcgggcga catgtttccg gcgaaaaaac tactgatata
     2881 aaccggtttt gctgaaacat cgctaatcag ccgctgcgcg ttgtttaacc atgttagcaa
     2941 aggctgacgg gcagcttcgc tgcgccagtt caggtcgtca atttccgcgc tgatgtacca
     3001 gccatccggc gttacgccag gcgcggcgct ccataatctg gcttgctgga gatcggcagc
     3061 cagcaggcga ttaagatagc tttccagcgc tgcggacgac tgtttctggt gcataaaaaa
     3121 ttccggatcg gcgttcagcc cgacaatcag cttcaggcca gcctgttgcg cagctgcggc
     3181 acgcttaaac aataacgcgc gctgttctgg ctgggtgaat gcatcgccgt aacgggtcca
     3241 ttgcaaaacc agggtatcga agccttgcaa acgtaactga ctcatcagcc cctgccactg
     3301 ggtatcggta acctgactat ctcggttttg tggttgccag ataatacctt tcatcgcaaa
     3361 ggaaaaaggg ctgaccaaaa gcagtgtcag caatacgaaa atgaacttac gcatttacca
     3421 gtgcactcca atggtgagaa acgcgttgtt gcgctctccg ttacgttgat taatcgcctt
     3481 aaaggtatgt tgatactcga cgccgagact gactttgtgc ggccaggcgt cgtagtgcgt
     3541 ctcgccggtc cagatattcc agcggacccc gactccgcca agctgcgcgc cctgagtgcc
     3601 tttatcacga tagccgttgt cctgaacgtg agcgtaaggc tcaatagtct gtccgttagc
     3661 taccttctga tgccagctga cgcgataatc tgccgtccac gcctgaatat cctggcggat
     3721 atattgcgcc gcatcgaggt acaggttttg ggcaaaccag cctgaaccgt tcgggtgcca
     3781 ttcgtcgctg tatttgccgc cattaaagaa tgaggcgctg gcgcgcagca tggtatcgga
     3841 tgcgccattt tggccgttca gcggcaactg ctgttcgacg gcgaggaaaa agatctgatc
     3901 gcgcagcggc ttccagcgca gaccggtgcc ggacatcgga tttttcaccg gcatcatcac
     3961 cccgttttct ccggtatcgg caaagacgcg gctataaact gagagcaggt cgccttccag
     4021 cagcatattg cgtccgatgc ggtactcggc ttccagttgc ccgtagctac gatagctttt
     4081 ccctggcgct gcgccgccga cattattgtt agcggtgctc attgccccgg aacgcaagcc
     4141 gatagaagaa tcgaaactga acgtccagcg gcgaccgacc tcctcatgca aacggcggaa
     4201 attgaagcgt tgctgatttt gctctggggt cagtggggtt atcagcgcct gattatcaat
     4261 gtcatcaatc accagccggg cgtagtgctg cgtcgcaggc atgtcatcca gacgctggtt
     4321 cacgtaggcc agttgtcgga tcagtgccgg atcgtccgga agccctttat gcgcctgttc
     4381 gagcatttcc cgcgactgtg cgatatcacc gctatcccac aaggcgtaac caagcgctgc
     4441 ctggatgttg ctattattcg gttccagttc cagcgcggcg cgcaaatcac tcaccgccgc
     4501 cgggacatta tgacgttggc gataaattgt cgcccgcgca acgtaagcgt tggcagaagg
     4561 cgcaatattg attgagcgcg tgagatcgct cagtgcgagt tccggctgac caggaatgta
     4621 acgttgcgca tgcagccacc agtagagggc attgtttccc agtccacgtt gttctgcctg
     4681 ttgtagccag cgatcgcgag ctgcaccatt tcctgccgcc tgggcggtat tggcagcagc
     4741 aagcagatcc tcattgctca tgtcgtgaag actgattttc tgccaggccg ccagtgcggt
     4801 ggcgtagtcc tcaacctgat acgcctgata ggctaccgca cgatgttgcc aggcgttcgg
     4861 ttgtcgttgt tcggcctgaa gccatgcata caaggccaca ccgggtagcg tgtcccgata
     4921 acactttgcc agacggttcc aggcggcggc atcgtaggaa ggcgacatat cgcccagcaa
     4981 gcgaactatt gccgggcaat tatctgcaat acccggcaac tggctttgcc actgacgttg
     5041 cgccgccagc ggcaagggtt ttgataaaat cgccaccttc gctggagttg ccaggtaagg
     5101 atgactttcc agcagagacg ccagtcgcgc cattaaagtc tggctgacac gcgcatcgcc
     5161 ctggaaagga tagcgttgca gcaataaatc ggcagcttcg cgtgactgct cgttctgcat
     5221 cagttgccag gtcagttgat ctaggcgggt aagatttgcc ggttcttgct gatacagcaa
     5281 tcgtgccaga cgcagagatt cagccttgtt acgggtcgcc acgctgacag cataacgctc
     5341 ctcaagcatt tcattggcgg ggagggtggc gagcagtttt tgcgctgcgt cgtactgacc
     5401 ttcttttaac agcaccggta gcgtcgcgcc aacaacatac tggcggttat cggcaaactg
     5461 caccgtataa ttcgccaatg cctgaacggg gttggcgctg tatttagata acagatagag
     5521 ccaacttttc tcttgtgcgt ccgtggtaaa cagcggctta ttttcaatga gataatgctg
     5581 gaggcgtgct ttttcgccac gataagccag cgcggtcgcg taagtaatat atgactgagg
     5641 atcggtgaag atcccctgtg attgcagtgc caggatccga tcgtccagct gcccggcaag
     5701 aagcacgtca aaccactgac ggcgttctgc cgcgcttaat gtgttctgct ggcgtgcttc
     5761 attgtatagc gtatctgcct gggaccattg tttcaggtag attgcccgtt gcagcagatc
     5821 ggttcgcagc gtttttcctt ccggcgatgc agcaaacgtc gcatcgttca gttgcgctct
     5881 ggcgacaggt aactgtgcca gccgcagggc attctgcccg acttcactgc gacaacgcag
     5941 ggtcggcgca gcatcgcacg ctttttgctg ggcaagcagt tcttcaacag tcgtaacgct
     6001 tttcacttca actggaatag ccgccagact gcgctcaagt cgggcatctc ctgggtgacg
     6061 tttcagttga tcctccagca acagccgcgc ccggtcatca tgaccaaaat ggcgataggc
     6121 ttccgcaagg tataaagtca gcggaatatt atccggcacc tgctggtgta tatattcaaa
     6181 ttcgcggatg gcggttgctt cgtcgttatt tttctgtgcc ttcagcgcct tatcgagacg
     6241 gggataaata acaaaatggc gataatcgct cagccccagc tcttctgcgc tggtgccgat
     6301 attgtctgcg agtgcgctgg tactcaataa agacgtcagc agtaaaccag accatccgat
     6361 gacgcgatta aggttattct ccttcatttt cggactccag ttgcgcaacc tgttctgtgt
     6421 ttaaacctgc tttgagtaat agtgattgca tcgaaacttg taattcgcgt tgaattgtca
     6481 ggacgcgatc caacgtttcc tggctgataa cgccttcggt gaccaaaaac ttgccgagcg
     6541 gcagagaact gcgttcatgg cgcaataaca acacgttaat tgctgaacga ttaatatgac
     6601 cgagcgtggt cagtatttcg gcgaacagga actgatgcgg cacatattgc cgccagattt
     6661 caccggtctg ctgttccgtg agccactgat gctgaaccgc attgtacaac attgcccgcg
     6721 gatcgtgacc gcgtcggcgt gcataccagt gacgtaaccc cgtgacaatt tgtccccgca
     6781 gaacaatgac gtaacgcact ttgcgtccga ctttacgcgt cagggccgcc agcgaaacag
     6841 ggtcaatacc atcttcactg ccgacaatta actcatcatt ttccagacgc agcggcagta
     6901 ctgcataatg cagcgctacg gaggctggca tttcggcaat cagcgaggac gggatctgcc
     6961 aggcatcgat ggattcccac gccacgccgt tttgctctgc cagcgcctgt gccagctgct
     7021 cggcgctaat cagcccctgc atcagcattg aaccgcccag gcgtagacct tcgacgcgat
     7081 tacgcagtgc tgtatcgagt tgttcttcag tgatgacctg attttccagc agaatttggc
     7141 ctaacgggcg caacgagcgg gtatcgccag tcacgctggg gaagtcatgc gttgttttat
     7201 cccacgcgac gcgacgtgga tcgccgtgtt gaagtacctg ttttagcgcg cgccagttgg
     7261 ccatgaagtt aatcaggttg ccccagaaaa gacgcaggac agatagcagc ccctgcgtca
     7321 gaccgtagta gccagtgacg aaaatcaccc gctgcacgat gcggttaaac atcaagccaa
     7381 agtttagcca cagcagggtc attaaccatg cgctgccgct aaaaatagaa aggaaatgcc
     7441 aggcatcggg ccacaaactt tcatacgcca gcaacagcaa aagctggagc atcaccagca
     7501 tcgcgaggaa gctgacaaag ttactgattg cccctttgcg gtcgcgccag agaaagtagt
     7561 tcagcgtcag gctggaggtc catttgtggg ttttaaagcc ctggaaaaca atgccgatga
     7621 tccagcgaga tttttgtcga actgcagtcg aaaaggtatc ggggaaatat tcgcgcacgc
     7681 agatcatgtt tgacgtccgt gcgtgctgta aaaatttacg ctgctcgcgt tctttggctt
     7741 cgtccaccac cggaaaacgg acaaaaattt ccgtcatacc tttctctttc aggcggaaac
     7801 caatgtcgta atcttcagtc agactctgca cgtcgaaagc aataccgtca ccgtcagcta
     7861 acagagcggt cacggcgcgg cggctgaaac aggtgccgac gcctgcgctg ggcacttgtc
     7921 cggcgagggc ttcacgcacc ggaacatctt tgccatgcag ctctgaaaac tcatcaatgt
     7981 aagtcatgct ggtgaagtgc gtccattcgc gttcgaacgg atacaccggg atctgaatca
     8041 gatctttacg ctcgaccaga tagttgaaca gacgcaattc catcggtgaa atcacatctt
     8101 cggcgtcatg cagaataaaa ccagcaaaag cgaaattggc gctacgttca aactgggtga
     8161 tggcgtccag cacgttgttc agacagtcgg ctttgctggt gggaccagga cgcgcgcaga
     8221 ctaccttatg cacgttcgga aagcgagcgc acacttcgtc aacatcacgt tgagtatcgg
     8281 ggtcgttggg gtaggtgcca acaaagatat gatagttttc gtagtcgagc gtggtcgccg
     8341 ccagctcggc catattgccg atgacgcccg tttcattcca cgccggaacc ataatcgcta
     8401 acggtttttc atctggttta tacagttcgc ggtaactcat tcgcgggtag cggcgataaa
     8461 cactcaactt gcgtttaatg cggcgtaccc agtagacgac atcaataaaa aaatcgtcca
     8521 gcccgctgat gaacatgatg accgctaacg ttatcgcgat tacttttaag ccgtagagcc
     8581 aggttgcaaa aacatcaaga agccagtcca cacaaaaacc ttacattaac gctggttatg
     8641 tttagggtgg cgtatattaa ggttttttat gaattgtgac agctttttac cattaatagg
     8701 tatgactatt gcggcacgtt atttttacac tggttataaa agttgccgtt tgcgtagaat
     8761 gtagacaaaa gcatggtgtc aaggagggga ggttaacgtc atagtgaaaa ggagcggtgg
     8821 caccgagcag gtcataatgt agtggaagtg cctggaggag gtgagcaggc agggcaagat
     8881 tacgaaagcc cgctccccgc aaggactgac gccagatagt ttctgtccat ggctgctttt
     8941 cgcatcttac gtcttaaccc tgccttgaat accttatcat tcgttaaaat attaatagcg
     9001 atgtgccgta tccctgaaaa taattctgcg gcgtttcctc ttcttatttt gcagtcgtct
     9061 tcattcatta ccacgtccag acgccagtgc agcttattct ccacgtgcca gtggtttcgg
     9121 attgctgtgg cgaacttctc tgcggttaaa tcagcagaac tgatataata tctgaccgtc
     9181 atttcgggct ctttcttttg ttctgctatt attgaccgaa aggagactgc cacgcataat
     9241 ttcttcagac ctttccattc aaacgtgaaa tcaataagtt catcagggac atcgcaaaca
     9301 atatgaagac ggatttcttc tctgccgtga ctcttttcac tgattgcgta actgtcatgc
     9361 tctggattat ttaattcttt cagcggaaat tttccctcaa aggctttatt tagccgcccc
     9421 tggtttcctt ttaccgcgaa taaataatca cctccctgtt tttgtatctt ctctgcaata
     9481 tctttctggc aacccatcgc atcagttgtg atgatttttc ctttaatatc cagcatgtta
     9541 agaagttctg ggatagctgt aatctcatta gatttctcat ccgtcttgat ctgtccgatg
     9601 accagactgt gcattgttga gaacgcacta atgacatgaa tcgctcccct gcggcgactc
     9661 ttgtcataag agtgccggag cgtttttcca tcaattgcaa tgacgtcttt atcatttgat
     9721 gaatggcagt cacgcatcca gttaataaag cactcg
//
LOCUS       DAZFRJ010000166         9533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000166 DAZFRJ010000000
VERSION     DAZFRJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.168"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..506)
                     /gene="mocA"
                     /locus_tag="ACHKUN_003714"
     CDS             complement(<1..506)
                     /gene="mocA"
                     /locus_tag="ACHKUN_003714"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO6661905.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIE"
     gene            609..1379
                     /gene="yqeC"
                     /locus_tag="ACHKUN_003715"
     CDS             609..1379
                     /gene="yqeC"
                     /locus_tag="ACHKUN_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO6661906.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1427..3052
                     /gene="yqeB"
                     /locus_tag="ACHKUN_003716"
     CDS             1427..3052
                     /gene="yqeB"
                     /locus_tag="ACHKUN_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO6661907.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3093..4025)
                     /gene="arcC"
                     /locus_tag="ACHKUN_003717"
     CDS             complement(3093..4025)
                     /gene="arcC"
                     /locus_tag="ACHKUN_003717"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6661908.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALLDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4073..5458)
                     /gene="hyuA"
                     /locus_tag="ACHKUN_003718"
     CDS             complement(4073..5458)
                     /gene="hyuA"
                     /locus_tag="ACHKUN_003718"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO6661909.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5511..6722)
                     /gene="ygeY"
                     /locus_tag="ACHKUN_003719"
     CDS             complement(5511..6722)
                     /gene="ygeY"
                     /locus_tag="ACHKUN_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO6661910.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6780..7976)
                     /gene="dpaL"
                     /locus_tag="ACHKUN_003720"
     CDS             complement(6780..7976)
                     /gene="dpaL"
                     /locus_tag="ACHKUN_003720"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO6661911.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8034..9221)
                     /gene="ygeW"
                     /locus_tag="ACHKUN_003721"
     CDS             complement(8034..9221)
                     /gene="ygeW"
                     /locus_tag="ACHKUN_003721"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO6661912.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2217 a         2327 c         2508 g         2481 t
ORIGIN      
        1 ttttctatct cgacggaata atgagctccc ataaggagtg cctgacgcat attggtgaca
       61 ttgggtcgtt ggattgcctg catcaagcat ggttttgaca ctaaaatcgg atggccgggg
      121 atgccattat ggagtggcag tattgcgcca tcatttcgta acgaccagat ttttctaaaa
      181 atatcgatgg tgagggttgg catgtcaccg tgggtgagaa aacaatgttc tgtttgcact
      241 gcgggtactg cggcctttac tgatgttagt aaaccctgcg cataatctgg gttgtggata
      301 atagtgatat tgctctggtt cgcataacgt tcgtgcagtt cattaccacg atagccggtg
      361 actaaaataa ttcggctaca aaactgcaac gcatttttga tacttgtatc aagaattgtc
      421 ccctgttgcc agggtaacat cattttccat tgccccattc ttgatgataa tccggcagcg
      481 gtaattatac agtcgatggc tgacatagtg gttccattat ttataggggc taataacgcc
      541 tgataattgc gttacttcct gatggattaa gttggggata atttatccca gagaggtcat
      601 aaagactcgt gaaaagtata gttgacccat cggcattagt cattgattta ggtgcgcaga
      661 aacgtcccac tgtgatttct gttgttggtg ctggggggaa aaccagcttg cttttttggc
      721 tggcagaact gctccaggct agcggcaggc gtgtgttaat cacgacgacg acacatatgt
      781 ttatgccaac gtctcactgg cccgtggttt tctgtcgtga tcccgccatg cttcctcatg
      841 cgtctcttac atcccccatt tcattttgtt ttcacagctg gaaagcgaac cagggaaaag
      901 tgcagggatt tacgccagaa gcgattgatg cactggtgca acgaccagag tgtgacgtaa
      961 ttctcattga agcagatggc tcgcgtggaa tgccgttaaa agcgcctgat gagcacgaac
     1021 cttgcatacc caaaagcagt tgctgcgtga ttgctgtgat gggaggacat actttgggcg
     1081 cgaaagtgag cacagaaaat gtccatcgct ggtcgcagtt tgctgatatt accgggttaa
     1141 cacctgatgc aaccttgcaa ctgagcgatc tcgttgcgct ggttcgccac cctcaggggg
     1201 cgtttaaaaa cgtaccgcaa ggttgtcggc gagtctggtt cattaaccgt ttttctcaat
     1261 gtgagaatgc gattgcacaa agcgagctcc ttcaaccgct gcaacaacac gacgtagagg
     1321 caatctggct gggcgatata caagaacatc ctgcaatcgc gcgcagattt gtgaattagc
     1381 ggcccgggta ttccgggtta ctgaaaggat atccattgag gttggtatga atattttcac
     1441 agaggctgca aaactcgaag agcaaaattg tccgtttgcg atggcgcaaa ttgttgatag
     1501 ccgaggctcg actccccgcc attctgcaca aatgttagtg cgcgccgatg gttctatcgt
     1561 cggtacaatt ggtggcggaa tggttgagcg gaaggtgatt gaagagtcac ttcaggcatt
     1621 gcaggaacgt aagccgcgat tattccatgg acgtatggct cgtaacggtg cggatgctgt
     1681 cgggtcagat tgtggaggtg caatgtcagt atttatcagc gtccatggta tgcgtccacg
     1741 tctggtgttg attggcgcgg ggcatgtcaa ccgggcgata gcccagagtg cggcgctatt
     1801 aggatttgat attgccgttg ccgatattta tcgcgaaagc ctcaatcctg aactattccc
     1861 tccatcaacc acgcttctcc atgctgagtc gtttggtgca gcagtggaag cactggatat
     1921 tcgccctgat aattttgtcc tgattgccac gaataatcag gatcgtgaag ccctcgacaa
     1981 actcattgaa cagcccattg catggttggg gttgctggca agtcgccgca aggttcagct
     2041 tttcctgcgt caattgcgtg agaaaggcgt ggctgaagaa catattgccc gtttacatgc
     2101 gcccgttggt tacaacatag gtgcggaaac gccgcaggag atcgccatca gcgtgctggc
     2161 agaaatatta caggtgaaaa ataacgcgcc gggtgggctg atgatgaaac cttctcatcc
     2221 ttccggacac cagctggtgg tgattcgcgg tgcgggagat atcgccagtg gtgtggcgct
     2281 acgtctgtat catgcgggtt ttaaagtgat catgctggaa gtggaaaaac cgacagtgat
     2341 tcgttgtacc gtggcgtttg cccaggccgt gttcgatggc gaaatgacgg tcgaaggcgt
     2401 tactgctcgc ctggcaacca gtactgcgga agcgatgaaa cttaccgaac gcggattcat
     2461 ccctgtgatg gcagatcccg cctgttcatt gcttgatgaa ctgaaaccgc tttgcgtggt
     2521 ggacgctatt ctggcgaaac agaatttggg aacgcgggca gatatggcac cagtaacaat
     2581 cgcgcttggg ccgggcttta ctgcagggaa ggattgtcat gcggtaattg aaacaaatcg
     2641 cgggcactgg ctcggtcagg tgatttactc tggttgtgcg caggagaata ccggtgttcc
     2701 tggcaatatt atggggcata ccacccgacg ggtgatccgt gctcctgctg caggcattat
     2761 gcgatccaac gtgaaattag gcgatctggt gaaagagggc gatgtgattg cctggattgg
     2821 tgagcatgaa attaaagcac cgttgacggg gatggtgcgt ggcttgttga acgacggcct
     2881 ggcagtggtc ggtggtttta aaattggtga tatcgatcct cgtggtgaaa cggctgattt
     2941 caccagcgtt tctgataaag cccgggcgat tggcggcggc gtacttgagg cgttaatgat
     3001 gttgatgcat cagggcgtga aagcgacaaa agaagtgctg gaagtggctt aaatgaaaat
     3061 ggccggatgg tattacccac ccggccttaa aattactgac aaatcaccgt tccggttttg
     3121 ccttcaatcc cctctttcgc tttgctcaat acggtaatca gtgcttcacg acctgcgcga
     3181 gagcgagcaa acgaggcggc agcttccact ttaggcagca tagaaccttt cgcaaaatga
     3241 ccttcttcga taaagcgttc agcatcactt aatgacaggc gatcgagcca ctgttcattc
     3301 tctttaccga agttaatggc cactttttct accgcagtca gaatgatcag catatcggca
     3361 tcaatcattt ctgctaaacg ggcgctggcc cagtctttat cgataaccgc gctggcacca
     3421 cgcagatggt taccttcacg aataacagga ataccaccac cgccaacggt gatcaccacc
     3481 tggcctgcat ctagcagagc tttaaccgtt tctttttcaa taatatcaac cggttttggc
     3541 gaggcaacaa cacggcgata gccgcgacca gcatcttctt tcagagtgta accctgtttt
     3601 gtcagttgct ctgcttcttg ctcggtaaag aacgaaccga tcggcttggt cgggttgagg
     3661 aatgccggat cgttagcgtc gacttcgacc tgagtaacca gcgttgctac cggtttatta
     3721 atgccccgag aaagcagttc ttctcttaag gcgttttgta gatcgtaacc aatataaccc
     3781 tggcttaagg caacgcagac agacatcggc agcattggcg agtgcgcttc agtttttgca
     3841 gcagcttcaa aggcctggtt aatcatgccc acctgcggcc cgttaccatg agtgacgata
     3901 acttcatgtc cttgggcaat taaatcaaca attgcctgag aagtaatttt taccgctttc
     3961 atttgtccgg ccaggtcatc gcccagcgca ttcccgccca gggcgagaac aattttctta
     4021 ctcatttttc ctcactcatc attcttgatt gtgtatagat ccctgacagt ggttagagca
     4081 cgggagggac aaacggtttg cgtcgcagga aacgacctcg cccggctttg cctgtaaagg
     4141 tgccgtcaca gaaaatcgtt tcaccacggg ataaggttct gacaatcgcg ccctgacagg
     4201 taaaaccctc ccatggcgag tagtcggcgt tgtcgtggag atggcgatgc tgaatttgtt
     4261 ggctctgacg tgggtcgata atcaccacgt cgccatcgga accgggcgct aataatcctt
     4321 tttgcggcca caggccaaac agcctggcgg gcattgcgct ggttaattca acaaagcgtt
     4381 ccggtgttat acgtcccgtc atcacgccac tggaaaacag taactgcatg cggttttcca
     4441 caccgggtaa gccatttggg cagcgactga aatcgccttt agaaatttgc aggcgttgag
     4501 ccatcgagaa ggtgcagtga tcggtcgcca ccacgtcaat cgcaccatcg ctgatgccac
     4561 accacagttt gtcctgctcg cgtacgttac gcagcggtgg gctaagaatg aacttcatgc
     4621 cgtcttctgt atcgtaactg cgttcgtcca acaggagata ttgtgggcag gtttcaaccc
     4681 agactggctg gtgattcgca cgggcaagac gcagataatc cagacctaag ccgttagaca
     4741 ggtgcacgat atagagcggg gcgttaccgg caatttgtgc caggttaatc atgcgggcga
     4801 tggcttccgc ttcgcattcc agagggcgac tcaaggcatg atagcgcggc gcggttaacc
     4861 ctgcggcgat aaattccgcc cgcttgctgg cgatagccgc atcattttcc gggtgcacgg
     4921 tggtcagcgc gccggattca tgcagacggc gtaatgcctg caaaacctcg tcatcgttga
     4981 gtttgtattg ataggttaaa tagagtttaa aactgctcag tccttcctcg accatcatcg
     5041 ggatttcgtc gaggattgcg tgattaatgt gctggatcac accgtgaaag ctgtaatcga
     5101 tgaccgcttt atgggcggca taaccacgat aaacctccag ttgatggcgt aaccgacagc
     5161 cgtttgggcc aaatcccata tggtcaataa tggttgttgt accgccacac gcagctgcgc
     5221 gggtaccggt aaaaaaatca tcacaactgc gcgcgatgcc gacatcaata ttgaaatgcg
     5281 tatggacatc cacgccgcca gggaaaacgt aacagccagt ggcatcaatt tcttcatacg
     5341 ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat cccgctttca atcagcaaat
     5401 cctgtttggc ttgtccatct gcgttaacga cagtgccgtt tttgatcaat acgcgcatag
     5461 caaactccaa aaaccctccg gcgaaccgga ggggaataag tgaagagtaa ttactctgtt
     5521 gccagccagc ttaacgggat tgcagcgtac atcgcggcac aggtcaccag gtgagatttc
     5581 caggtttttt cgttaggtgc atgcgcttca ggttctttac ccgggccaaa gccgatcact
     5641 ggaatgccgt gacggcccat gatagatacg ccgttagttg agaaggtcca cttatcaaca
     5701 accggcgctt tgccaaacag cccttcgtag gcattcacca gtgctttaac ggtgaagtga
     5761 tcttcttcca ctttccaggt cgggaagtag cattcggttg ggtaaaccag gccagtccag
     5821 gacggacggt cgtagttgta catagaaaca accgcgttag ctttctgtac tgcaggcagg
     5881 gcgcggattt cgtccagcgc gccttcccag gtttcgcccc aggtcagacg gcggtcaata
     5941 gagacggcgc agctatctgc tacagcgcaa cggcttgggg atgtgaagaa gatttcagaa
     6001 acggtgaggg tgcctttgcc gaggaattcg tcataaccca gacgttggga gagttcttgt
     6061 aattcgccaa gaatcggacc cattttgaaa atggcgttgt caccgcgttc tggcgcagaa
     6121 ccgtggcagc taacaccctg aacatcaata cgaatttcca tacgaccgcg ctgaccacgg
     6181 tatatctggc agtcggttgg ttcggtactg accacaaatt ccgggcgaat gccggattgt
     6241 tcaataatgt actgccagca cagaccgtcg cagtcttctt cctgcacagt accggtaacc
     6301 agcagggtat attcatcttc cagaccgagg tctttaatga ttttaccggc ataaaccata
     6361 gatgccatgc cgccttcctg gtcggaagta ccgcgaccac cgattagctc atcagtttcc
     6421 atgccttcgt acggatcgaa gtcccagttt ttgatgttgc caatgccgac ggtatcgata
     6481 tgagcgtcca ttgccaccag acgcgggccg tggccgatat aaccgagaac gttgcccatc
     6541 gggtcgattt caactttatc gaagccgact ttttccatct cttctttaat acgatgtact
     6601 acgcgttttt cgtcgcagct ttcactggga atagcaacca tgtcgcgcag gaagcgagtc
     6661 atgtccgcct ggtaatcttt tgctttttca agaatcagtt tgaatggaat attcttagcc
     6721 attatttttc tccagatttt ttactccccg ttttgcaggg agtagttcca atagagtgat
     6781 taaggtgcta cagcgtgttt gccttcccag acaacttcgc ggtagtgctt cacgtcggtg
     6841 tcgccttcag tgctgataac cagcaccacg gcatctttgt tcagcgccag tttttccatc
     6901 aggctttgac gttgcgggtg ataatgaacc gctgcgagaa cgcccaaacc gacagcgccg
     6961 gattcaccgg agatgatgcg cgggtcgttg ccgtacggat tacccagcac gcgcatacct
     7021 aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg cgcagttacg taggatttcc
     7081 cagcccagcg ggttaggttc gccacaggcc aggcctgcca tgatggtggc catatcaccg
     7141 ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa tacagtcagc tttgtcaggt
     7201 tcaacaataa tgctgtgcag attttgcggg ctatagacgt cgaccagata acccagcaca
     7261 ccaccggcca ttgctccgac accggcttgc agcagaacgt gcgtcggggt tacgcccatt
     7321 tcacgcattt gctcgacggc ttcatctgcc agggttgcgt agccttgcat gatccaggtt
     7381 gggattttgg tgtaaccttc ccatgccgtg tcctgtacca cttcccagcc gtgctgctgc
     7441 gcgtgttgca tggtcaggcg aacggtatcg tcatagttca tatctgtgac gatgcactcg
     7501 gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag aacctttcgg catgtaaatc
     7561 accgcattct gtccgagttg ctgcgctgcc cacgccacac cgcgcccgtg gttgccgtcg
     7621 gtggtggtcg cgaaagtcat tttttcgccg atggcatttt tcaggtgctc aaatgacagc
     7681 gtttcgatat caagatgata tttttcacac aataattgag cgatagcgta cgcaccgccg
     7741 agcattttga aggcgttcag accgaatcgt tttgattcgt ctttgacaag aattttcttc
     7801 accccaaaaa ggtttgcgag atcgtccagc gcacaaagcg gtgttgggcg ataaccagct
     7861 attttctggt ggaactggcg cgccagtttg gcctggcttt gcgaaaagag cggtgatgtt
     7921 tcgccgttga aaaatttgtt atcggcgata tcaatcttca atgagaaaac ggacatatcc
     7981 tatccttttg gcctctggaa atagagggcc gaccagctga tcggcccgag gggttatttc
     8041 acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg cacctggttt ggcgtattta
     8101 cgggacagga tcatcgcggc gatgatataa ggcttccagc tggcttcttt gtaggtagcg
     8161 atacggtatt tttcgaatac gccttcagtc acttcccctt ctttgcagga tacgccgctg
     8221 atatcagctg gcaggcagtg catgtacagg gcttcgccat cacgggtcag ttccatcatc
     8281 tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg ccagacactg tttttccagt
     8341 gcttttaagc cttcgtgatc gttagcgcgc agcaattcgg tacgctgttc catcactttg
     8401 taaggtgccc atgacttcgg ataaacgatg tctgcatctt tgaacgcttc ttccatgctg
     8461 gtgacctgac ggaagctacc gccagatgct ttagcattgt ttttcgccac ttcaaccaca
     8521 tccgggatca ggtcgtagcc ttccggatgg gccagggtga catccatacc gaagcgagtc
     8581 atcagaccga tgatgccttg tggtacagag agcggtttgc catagcttgg agagtaggcc
     8641 caggtcatgg cgattttttt acctttcagg ttttccagtg aaccaaagtg ttcacgtaac
     8701 cacgcgaggt cagccattga ctgagtcggg tggtcaatat cgcattgcag gttcactaaa
     8761 gccggacgct gtggcagtac accctgcttg taaccgtcat caagtgcagc gccaacttca
     8821 cgcatatagg cgttgcctgc gcccagatac atatcgtcgc gaataccaat agcgtcggcg
     8881 cagaaggaga tcatattggc ggtttcacgc acggtttcgc cgtgagcgat ttgtgatttg
     8941 ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa gcgcggaagc ataagagaag
     9001 cgggtacggg tggagttgtc gcggaatacg gaaataccta atccactatt aaagactttg
     9061 gttgagatgt tttcagcacg cagtgctttt aatgctgcgg caacgtccag tacttgtttc
     9121 agttcatctg gcgtctgttc ccacgttaac aaaaaatctt tctcgtgaag gtgagaggtc
     9181 agcgaattga tatccttaat cagctcatta acagttttca tcataaagtc ccggtagtgg
     9241 aatgacaggc aaagtggttt atttcactgt gacttgccaa agtgattggt gttttcacag
     9301 ctatacctgg gactaacaat aagtgtgcca gaattatcca cggggctgca aaagactaat
     9361 ttgacggata atgtgtgggg ttgatcacaa attgatgcca gcttccggtt gtttgtaaaa
     9421 tacgaaaacg tttggctttt tttatcgcgt tatttatttg ctgctaagta tcagaaataa
     9481 gataaaggga caggctatct ctcttcagtt tgataaactc accgccgtaa ttc
//
LOCUS       DAZFRJ010000167         9459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000167 DAZFRJ010000000
VERSION     DAZFRJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.169"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..286
                     /gene="ghoS"
                     /locus_tag="ACHKUN_003722"
     CDS             <1..286
                     /gene="ghoS"
                     /locus_tag="ACHKUN_003722"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6661913.1"
                     /translation="NKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPR
                     QLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            314..487
                     /gene="ghoT"
                     /locus_tag="ACHKUN_003723"
     CDS             314..487
                     /gene="ghoT"
                     /locus_tag="ACHKUN_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO6661914.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(606..2123)
                     /gene="lysS"
                     /locus_tag="ACHKUN_003724"
     CDS             complement(606..2123)
                     /gene="lysS"
                     /locus_tag="ACHKUN_003724"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6661915.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2360..3817)
                     /gene="dtpC"
                     /locus_tag="ACHKUN_003725"
     CDS             complement(2360..3817)
                     /gene="dtpC"
                     /locus_tag="ACHKUN_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO6661916.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(3876..6023)
                     /gene="cadA"
                     /locus_tag="ACHKUN_003726"
     CDS             complement(3876..6023)
                     /gene="cadA"
                     /locus_tag="ACHKUN_003726"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO6661917.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(6103..7437)
                     /gene="cadB"
                     /locus_tag="ACHKUN_003727"
     CDS             complement(6103..7437)
                     /gene="cadB"
                     /locus_tag="ACHKUN_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO6661918.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7803..9341)
                     /gene="cadC"
                     /locus_tag="ACHKUN_003728"
     CDS             complement(7803..9341)
                     /gene="cadC"
                     /locus_tag="ACHKUN_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO6661919.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         2665 a         2190 c         2139 g         2465 t
ORIGIN      
        1 aaacaagttc aatacttatg ttgtttcttt tgattatcca tcatcttatt cctcagtgtt
       61 cttaaggtta agatcattga tgtatgatat gaatttctct tctatcgtgg ctgatgaata
      121 tgggatacca cgacaattga atgaaaattc cttcgcaata acgacatcgt tagccgcaag
      181 tgaaatcgaa gatttaatca ggcttaaatg cttagactta ccggatattg attttgacct
      241 caacattatg acagttgatg actatttccg tcagttttac aagtagccag gaaggataag
      301 aagaacataa aaaatggcac tattctctaa aatattaatt ttttatgtga ttggtgtgaa
      361 catatccttt gtcattatct ggtttatctc acatgagaaa acacatattc gtttacttag
      421 tgcattcctg gtcggaataa cctggccaat gagtctgcct gtggcattac ttttttctct
      481 cttttaggag ctgtagttga gtggacccgc tggttatcgt gaaacgaagc gtctctcctc
      541 aaaactggat ttgcggaacg acgaacgaag cagtcgcctg actgcttcgt tcattaaagt
      601 gaaatttatt tctgtgggcg catcgccggg aagagaataa cgtcgcgaat agtatgactg
      661 tttgtaaaca gcataatcat tcggtcgata ccaataccca gaccagcggt tggcggcaga
      721 ccatattcca gcgcagtcac gtaatcttcg tcatagaaca tggcttcgtc gtcacctgca
      781 gctttagcgt taacctgttc ctggaaacgt tcagcctgat cttctgcgtc gtttaattcg
      841 ctaaaaccat taccgatttc acgaccaccg atgaagaatt caaaacggtc ggtgatttct
      901 gggttaacat cattacggcg tgccagcggt gacacttctg ccggatattc cgtaataaag
      961 gttggctgaa tcagatgtgc ttctgccact tcatcaaaga tctccgtgac aatacgtccc
     1021 agcccccagc ttttctctac cgtaataccg atagattcag ctaatgcttt agcagcatca
     1081 aaattatcca ggtcggccat atcggtttct ggacgatatt ttttgattgc ttcgcgcatg
     1141 gtgagttttt caaacggttt gccgaaatca aacacatgct cgccataagt gactttagtg
     1201 gtacccagaa cctcttgtgc cagggtgcgg aacagggact ctgtcagttc aatcaaatcg
     1261 tggtaatccg catacgccat gtagagttcc atcattgtga actcaggatt atggcgaaca
     1321 gaaatacctt cattacggaa gttacggttg atttcgaata cccgttcaaa accgcctaca
     1381 accagacgtt tcagatacag ctccggcgcg atacgcaggt acatatctaa atccagagcg
     1441 ttatgatggg taataaacgg gcgagcagat gccccacctg gaattacctg catcatcggg
     1501 gtttctactt ccataaagcc gcgcgcgacc atgaactggc ggatagcggc cagaattttt
     1561 gaacggacaa caaacgtttg acgggattta tcgttagcga tgaggtccag ataacgttga
     1621 cgataacgga cttcctgatc ctgcagacca tggaatttat ctggtaaagg acgtagtgct
     1681 tttgtcagca ggcgcaactc agtacagtga atggaaagct caccggtttg cgtcttaaac
     1741 agcgtaccgc gggcaccgat aatgtcaccc agatcccatt ttttaaactg atcgttataa
     1801 acaccttctg gcaggctatc tcttgcaacg tacagttgaa tacggccacc gacatcctgc
     1861 aacgttacaa aggaggcttt ccccatgata cgacgggtca tcattcggcc agcaaccgag
     1921 acttcaatgt ttaaggattc cagttcctgg ttatccttcg catcaaactc ttcgtgcagc
     1981 tggtcagagg tatggtcgcg gcgaaaatca ttgggaaacg ccacaccttg ctgacgtagt
     2041 gccgccagtt tttcgcggcg atttctcagt tcatcgttaa aatcaatagc ctcattggct
     2101 ccccgtgttt cttgttcaga cattttggtt cctctaaatc cagctttcaa tttagcgtag
     2161 ataaagagac agatcggtac actgtattat ctgcctcgac tattaataac tcagtttatc
     2221 ttatcgaaac taacgcttac ctcaagttga tatcactaat aaataaccat caaaatggtt
     2281 taaatgactt attaatctag atttgctagt gttataaact gaaagttaat ttattcataa
     2341 ccaattgttt ttacgacagt taatcgttgc tctcctgtat catattcgtc ggcgtgctaa
     2401 acagaaaacg ggtagcaaag gccaatacga cgatgatcgc gacacaagcc aacgtccact
     2461 ctcccatctg agaaaagaat cgctggtaag ctgcaattgc cataccgcta atttgcgact
     2521 ctgtcgtctg ctgtgccacg acgcctgcca gccagttggc gaccgcgcct gttgccagca
     2581 tataaatacc ggttaatacg ccagacattt tcagacgcgt gatttgcgca atcgccaccg
     2641 ggtcaataaa gagttcggca aagcccatta gcgccagccc ggatatcatc acggccattg
     2701 acgcttgacc gccagccgct gcatgtcggg catcaaatgc caacaacata aagccacaag
     2761 ccatcagcag taagccaaag gcaaacttca gccagacgcg caatgttgag ttgccgcggc
     2821 tttctggcga cgccagccag gccagtacaa ccccagcgag catcactgca attgcattca
     2881 ccgactggaa tagtgctgta ggtacttcaa tattgaatgc ctgacgattc acaaagcgat
     2941 cgataaacaa gctgatggta ctgccgccct gttgtgccag tacccagaac aacgtcccga
     3001 caaacatcaa caatacaatt tgccaaagag cacggcggtg ttcggggaat ttgatcatca
     3061 tgcgggcaat gatttgtgcg gcaatgaggc aaacgatcgc cagcaaatat ccggaccagt
     3121 cgttctccag cagcagggta aaaaatactg gggctaaaca gagcatcacc actaaccagc
     3181 tccatactgg taaggcaaat ttcacgctag tgagcgcttt tttatccata ctacgcgtgg
     3241 actggaaatg acgatgcccg cttaagaaaa tcaacaaacc gataaacatg ccgccacccg
     3301 caagggcaaa gccaacatgc catccatacc actgagcagc caggccgcag gcgattgggg
     3361 cggcgataga accgatattg cccgcagcat acagcagcga aaaaccgcca tcacgtcgat
     3421 gatcgttctc gtcgtagagc tcgccaagca aacagctgat gtttgatttg aataaaccgt
     3481 agccacaaat aatgattgcc agcgccagat acaggctaaa ggttgaattt gtatcaatgc
     3541 ccagcaccac atggccaagg gtcattaaca gcgcgccggc aatcactgca gtgcggttgc
     3601 cgagcaggcg gtcggcaagc cagccgccga gaataggggt aacgtaaacc agagaagcat
     3661 atgcgctgaa caggctgatg gcatggttat catcaaaacc aagctgatgg gtgagataga
     3721 gaatgagtaa ggcacgcatg ccgtaaaaac tgaagtactc ccagatttgg atcgccacga
     3781 tatagtatat cgcgcgcggc tgtgagggtg ttttcatgtg ttctccttat gagcaaaaaa
     3841 gggaagtggc aagccacttc ccttgtacga gctaattatt ttttgctttc ttctttcaat
     3901 accttaacgg tatagcggcc atcagcctga cggtatgcac cgtgaatatc ggtttcaaag
     3961 cccggatagt gagcgccgat ttcacacagc atctgcagga actccagaac cggacggctt
     4021 tcttcggtga tcatttcacc cggcattacc agaggaactc ccggcggata cggaaggatc
     4081 atattggcgt taatacgacc taccatttcg tcgaggtaaa cttcttcggt cataccgtgc
     4141 agctctttct ggaatgcagc atacggagtc attaccatcg tcggcagcac ttcaaatgcg
     4201 cggtacatca gatccggcag attgtggtga acaatcagtt tgtggatatt ctgagccagt
     4261 tcctgaatac gcatgttttc atagaattca ggatcttcac gatacagaga cggcagcatg
     4321 tttttcacac gcaggttcag gtcgaacgca cgtttgaagt cagtcagagc acgcagcagg
     4381 ctcagtgctt tggtcttatc gataccgatg ctgaacagga acagcaggtt atacggaccg
     4441 gttttctcaa caacgatgcc atgttcgtcg aggtatttcg ccacgatgct ggccggaata
     4501 ccaaagtcgc tcatggtgcc gtctttttcc atccccggag tcagcagggt gactttgatc
     4561 gggtcaagat acatgtgctc gttatcgatg tttttgaagc cgtgccaggt gctgtcagaa
     4621 cgcagcggcc agcattcagt cgtatcgata tgatccggct gccatacatc aaagaaccag
     4681 ccatcagatt ccgttctcag acgtttgatc tctttacgga atttgatcgc acgttcaatg
     4741 gaaccgttga tcagacgttt acctgcattg cctttcatca tcgccgcagc ggtttcagtg
     4801 gacgccacga taccgtagtg cggagaagtg gtggtgtgca tcatgtaggc ttcgttaaag
     4861 gtttcttcgt ttacgtcacc tttaacgtgg atcatggaag cctgagagaa cgccgccagc
     4921 agtttgtgag tggactgggt ttcgtaaatc actttccctt ctacacggcc accgctcata
     4981 ccgcatttac cttcgtaaat cggtgagaag ttggtgtaag gcacccacgc ggagtcaaag
     5041 tggatggatt tcacatccag tgttttcttg atgaagtcgg tgttgtacag cagaccatca
     5101 taggtagagt tggtaattac agcatgtacc ggccaggttg cgtttggtgt ttctttcacg
     5161 cgcttagcaa tggtagcgtg ctggaattca ctctgtggga taccaccaag aataccgtaa
     5221 gcgttacggg tcgggcggaa atagattggc gtaacatcgc tcatcatcat caggtgggtc
     5281 agcgatttgt ggcagttacg gtcaatcaga atggtgctgc ctgccggagc agagtacatg
     5341 ccaacaattt tgttcgcagt ggaagtaccg ttggtcacca tgtagctgcg gtctgcgtta
     5401 aagacgcgag cgatatactg ttctgcttct ttgtgtggac cactgtgatc cagcagagaa
     5461 cccagttcag atactgaaat ggaaatatca gatttcatgg tattcggacc aaagaaatca
     5521 tagaacaggc tacctaccgg gcttttctgg aatgcagtac cgcccatgtg accaggagta
     5581 cagaaagtat atttaccttc acgaacatat ttaaacagtg ctttagtcag cggaggcaga
     5641 atagtgttga tatattcgtc agtggtctgc ttgatcttat tagcaatatc ttcagcagca
     5701 cccagcgcat attcaaagaa gctaatctgt aaacgcaggt cattcaggct tacatcgaga
     5761 gtggaatacg tattagcgaa cgcgtacaac ggcaggttct cgttcatttt gctaatttct
     5821 tcgcacagct cgagattata tttatcccag tcaaaaataa cgccgcacag acgcgcattg
     5881 ttttcgatca gttttaataa gtcgtcacgg tcgttcgggt aaacaatctg gaagttcaga
     5941 cgttcaagcg cgcgatgaag ttcacggatg ggttcttctt taaaataaac ccccatgtga
     6001 ttcaatattg caataacgtt catagtcata tctccaggta aaaaaggccc ctcccaacac
     6061 atgggacaaa atgaaaggag gagcctcgga aaatactttt aattaatgtg cgttagacgc
     6121 ggtgtggtta tccattgagt ggctctggcg ctcgtgcatt ttgcgagcgt agaacatcag
     6181 gataatcagg ctgacgatga aggtacctgc cagctcgaag gagcttgcgc ccatcagcgc
     6241 gatgaagcag aacacacaac ccagtacaga gcagatcagg ctgacaaagt tgcggatgtt
     6301 aacgccttca aaacgaatca ggtcaacgca agagtagaag tacggcagca tagtcagcag
     6361 tactgcgata ccggtcagtt caccgaacag gtcagatgct ttaccaccgg cagagttcat
     6421 cagagtgatg aggatcatca gggcagtcat tttcactgca gccagcagca gacctttttt
     6481 cggaataccg ttgctgtcga cttcaccata aactttcggg aagttaccgt cgttagcggc
     6541 acgtacacct gcctggccta ccaacatcat ccaggagccc agagaagtca ggcacgcaaa
     6601 ggcggtgaat gcagaaacca gcggcgcagc ccagttaccg aggatggttg aagcactgat
     6661 tgcaaacgga gcaccggaag ctgccattac agaagatgga tacataccgg aaagcacctg
     6721 agtcgcagcg atgtaaacaa tacctgctaa accagtaccc agcatggttg ccagcggaac
     6781 ggtacgtttc gggtttttaa ccataccagt acttactgct gcggattcaa cacccacgaa
     6841 ggcccacagg cagagcagaa tacttttaat gatcgcatga ccatcagtgg tatccgcagt
     6901 attccagtta gctgcataag ttgccgcatc aaaccaatgc cagccaacaa tagcggtcat
     6961 tactaccgga ataagaacca gcaccagacc aatagtggtt aaacggctta cccaagtacc
     7021 gccgagcata tttacaaagg taaataccca gacgatagca atacaggcga tacccgccgg
     7081 aacaggatca tttaatactg ggaagaaggt ggaaagataa gataccgcgg taataccaat
     7141 cgccaggtta ccaatccagt tagcatggta ataaagaaca cctgtctgaa aaccaaatgc
     7201 aggggaaatt tctccggcat aagcaattgg gccaccttgt tgcgggtttt ttgttgccag
     7261 tcgggcatat acatacgcca gcgacattgc accaataata gagataatcc aaccccagat
     7321 agcaatacca ccgatacttg ctaggttcgc aggtaataat gcaataccgc tccccatcat
     7381 attaccggca acaacaccgg tacaggcaaa tagcccgatc ttcttggcag aactcatgct
     7441 cttctcctaa tttcattttt gaatttggag tccgggtcat gatgtataac tatttcctga
     7501 ccagaccaaa ctggcgctaa gaatactcac gaaaaaagga ttaatcctaa agattaggtg
     7561 aaataacaca aaagtttctg taagtgagaa cttgaggttt tttattaaca catcaggatc
     7621 gcaagttgat atcatgaaaa gataaacatt caatgtttac aatggattgc gggatattct
     7681 ctggttaaat ttatgtaata aaaattatgc ggcaaataaa ttgccgcaac atattatacc
     7741 aacagaaaca tacaaaaact caacaacaaa tattcccgag cataaatcaa cccgggagtt
     7801 acttattctg aagcgagaaa tttgtcgaga taaggtacaa cataaggaac agaagtctgg
     7861 aatataccat tttcaatcca gtaaagggtg tttgcccctg ggcgtaaatt aaaggcggtg
     7921 agatatgcat cagccgcttc ccggttcatc cccttcattt cataaacctt gccaagcaac
     7981 acataattta gccaggacat ttcaagatca atgccagtat ttatcgcctg gtaagactca
     8041 tctgttttac cttttaccag agcactgacc gcttttattt gatatataat ggacaggtta
     8101 ttcaattccg gcagtgtaac aatgttgtct atttctgtgt tcagtgctgc taattgtttt
     8161 tcatctaaag gatgttgaga atggcgcacg atatcaacta atgctttttc tgctctcgcg
     8221 taggtaaatt ctggggatga ttgaacaatc tcacctaata attcactggc acgattcaat
     8281 gatttatcat cgccatgcag taaataatca tgtgcctgat aaaaattagt taataacgca
     8341 ccacgatgag gcaatatttg ctggagcgtc tcctgcattc gttgtggcca cggttggttt
     8401 aacgcttttg ataaactctc cagtaaatca ttttgaatcg ccagctgatt accgttagtg
     8461 atgacataac gtttatccag catggttgaa ccatctgcat tgtctaccaa ttttatcgac
     8521 ataaagcatt gttgagcacg gtattggcgc tgattaacaa acgcaataga taatgtttta
     8581 ccggaactgc tcggttcatc aatgttgtag ttgattttgt catgcaccat aaaggtggag
     8641 aaggtgttaa gtgatgtcgc caccaaatca cccacgccta tcgcgtaaga gagctgatac
     8701 ggggaactcc agctgttaca acttttattt accatattaa tgtcaatatc gcgtggattg
     8761 agcaaaatac gcgatttgct cataggaaga cgtgtatcaa gagttgaaaa cgctaccagt
     8821 gctacacaga tacctaacga caacaggaaa aaaaaccata cccaaaaggt agtgaatcgt
     8881 ttgcttttaa ctggggattg ttcaggtggc gttgcggtgt tttgaatgtt aagactgtgg
     8941 gagggagaat ctgtggcagg aaccgcctct ggtatagggg gaggcgaaga tagcattatt
     9001 tcctctccct cttcttcgct gtaccagata accggcacca ttaatttata gccgcgcttt
     9061 ggtacagtag cgatatagac aggactatct tcatcattat cttttaatga cttacgtagt
     9121 tctgagatac tctgcgtcac aacatgattg gtgacaatac ttctcttcca gacattatcg
     9181 ataagttcat ccctgctaag tacttcgcca ctgtgttgag caaagaaaac cagaagatcg
     9241 attaatctcg gctcaagggt aagttgacgc ccattgcggc taatttggtt tatggacgga
     9301 gtaacaagcc attcgccaac gcgaactaca ggttgttgca taatagaaac tcattcgaaa
     9361 agggaatgat gcaatgataa ttgccacaac ctatttttac catctataga tgggtttatt
     9421 tacatattat tggtgaatgc aagacgttat ttttaccag
//
LOCUS       DAZFRJ010000168         9442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000168 DAZFRJ010000000
VERSION     DAZFRJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.170"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..1601)
                     /gene="yeeO"
                     /locus_tag="ACHKUN_003729"
     CDS             complement(114..1601)
                     /gene="yeeO"
                     /locus_tag="ACHKUN_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6661920.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1762..1837
                     /locus_tag="ACHKUN_003730"
     tRNA            1762..1837
                     /locus_tag="ACHKUN_003730"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1795..1797,aa:Asn,seq:gtt)
     gene            complement(1875..2825)
                     /gene="cbl"
                     /locus_tag="ACHKUN_003731"
                     /pseudo
     CDS             complement(1875..2825)
                     /gene="cbl"
                     /locus_tag="ACHKUN_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(2927..3844)
                     /gene="nac"
                     /locus_tag="ACHKUN_003732"
     CDS             complement(2927..3844)
                     /gene="nac"
                     /locus_tag="ACHKUN_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO6661921.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4170..4245
                     /locus_tag="ACHKUN_003733"
     tRNA            4170..4245
                     /locus_tag="ACHKUN_003733"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4203..4205,aa:Asn,seq:gtt)
     gene            complement(4289..5236)
                     /gene="ldtA"
                     /locus_tag="ACHKUN_003734"
     CDS             complement(4289..5236)
                     /gene="ldtA"
                     /locus_tag="ACHKUN_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6661922.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(5298..6377)
                     /gene="cobT"
                     /locus_tag="ACHKUN_003735"
     CDS             complement(5298..6377)
                     /gene="cobT"
                     /locus_tag="ACHKUN_003735"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO6661923.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCAAHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6389..7132)
                     /gene="cobS"
                     /locus_tag="ACHKUN_003736"
     CDS             complement(6389..7132)
                     /gene="cobS"
                     /locus_tag="ACHKUN_003736"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO6661924.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7129..7674)
                     /gene="cobU"
                     /locus_tag="ACHKUN_003737"
     CDS             complement(7129..7674)
                     /gene="cobU"
                     /locus_tag="ACHKUN_003737"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO6661925.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7826..7999)
                     /locus_tag="ACHKUN_003738"
                     /pseudo
     CDS             complement(7826..7999)
                     /locus_tag="ACHKUN_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7978..8166)
                     /locus_tag="ACHKUN_003739"
                     /pseudo
     CDS             complement(7978..8166)
                     /locus_tag="ACHKUN_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(9163..9442)
                     /locus_tag="ACHKUN_003740"
                     /pseudo
     CDS             complement(9163..9442)
                     /locus_tag="ACHKUN_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2596 a         2529 c         2015 g         2302 t
ORIGIN      
        1 gaactacaga ggaatcgtga gaacgaggcg aatattagcg atgcccaccc acaatgtcaa
       61 agcctgtttt ttaaatttga aatcgtttgc tgaaataatc tgcattttgt tgtttattcc
      121 gacacaactg gctttttttc acacttttgc ggttcgggtc gagggtattt ccatagccaa
      181 cgtccagtaa ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt
      241 cccatccaga caccaaccac accccagcca agcatgatgc ccagcacata accgacgaca
      301 acccggcaac cccacatgct caacatagaa acccacatag cgtaacgggc gtcacgagcc
      361 cccttaaatc cagcgggtag cacccatgag gcggaccaaa taggcataaa taaagcattt
      421 agccaaatca gaatcacaac gacatgttta acctgtggat cctgggtgta aaacgatgcc
      481 ataaccccgg caaagggagc cgttagccag gcgatggccg ttaatccaag agtggacagc
      541 cagaagacat gccgcaactg aatctctgct tgcgctatct gccctacccc taaccttcgg
      601 cctgtaatga tcgtagaagc agagccgagc gcacttccgg gtaagttgat aagagccgca
      661 attgaaaacg cgataaaatt tccggcaata acactggtcc ccatcccggc aacgaacatt
      721 tgggttaata accgaccact ggtaaataac actgattcga cactcgctgg aataccaatc
      781 cccatgactt cccagataat gctaaaattc agcggtttaa aatagctctt taacgaaatc
      841 cttagcgcag gattaaaacc aatcgccagc acccacaaaa ttgcaactgc gccaatataa
      901 cgagaaatgg ttaaacccaa cccagccccg acaaatccca gtcccggcca ggagaaaagg
      961 ccgtaaatca atatgccgct aataataata ttaagaatat tcaggctacc gttaatcagt
     1021 agcggtattt tcgtattccc tgcaccacga agtgccccgc taccaatcaa agtgatggca
     1081 actgctggat aactgagtac cgtcagctcc agataagtca acgccagtgc tttaacttct
     1141 gtcgtggcat cacccgcgac gaaatcaata atttgttcgc caaaatgatg aataagcgtt
     1201 gccaacagta cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc caccctcgct
     1261 cgtcgtcgat cccgcttacc gagactaaat gccacaacga cagtagtacc aagatcgata
     1321 gcagcaaaaa aagccataat gaccatattg aagctgtccg ccaatcccac gccggccatc
     1381 gcatcttttc ccagccagct gaccagaaaa gtgctcagga cacccatcaa caggacacag
     1441 gcattctcca tgaagatagg aacagcaagc ggggttatct cgcgccagaa caacactttg
     1501 tagctcttgc gtttagcgtg ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag
     1561 atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa
     1621 tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca aactgttgat
     1681 agaaacggca aacagttggg aaatttaaaa atcgggtttg acaaaagatt tttcgccgtt
     1741 aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     1801 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat ttctgaaaag cccgctttta
     1861 tagcgggctt tttgctatat ctgataatca atttcctctt cactgttctc catcacctgg
     1921 cgcttgatat cctcaactga cagtcctgca ttacaaagtt ccagaaagcg ccagacatag
     1981 ttacgttgaa gttgccctcg cttcagtccc aaccatacag tattggcatc gaaaagatgc
     2041 cgcgtatcca gacggattaa attctcttcc tcttgttcgc cgctggattg ttcggcaact
     2101 aatccaatcc ccagcccaag agcaacatag gttttaatga catcagaatc ctgcgcactt
     2161 aatacaatat ctgccagcaa acctttgcgg gcaaatgcgt catcaatacg tgagcacccc
     2221 gtaatcccct atcggtaagt gattaacggc cacttcgcta ttgattccag cgtcaacggt
     2281 gtaatttgcg tcaagggatg atcgagtgga acaagcaaac tatggtgcca acgaaaccac
     2341 gggaaggcga cgagatgcgg gtcattactc aaacgctcgc tggcgatacc aatatcagct
     2401 tcgccatttt gcaacaacgt cgcaatttcc tgtggcgtac cctggattag ctccagccga
     2461 acctccggga aaagttcgcg aaaagcttta atgacctcag gcaagctata acgtgcctga
     2521 gtatgcgtcg ttgcaatagt gagaacgcca gacgtatcgt tggtaaacag gtctgcaagc
     2581 cgacgaacat tactggcttc attcagaata cgttctgcaa tgaccagtaa tgctttgccc
     2641 ggttcagtca tacccagcag tcgcttacct cgtcgaacaa atatttcgat gccaagttca
     2701 tcccccagtt cccgaatatg acggctgacg ccggactgtg aggtaaaaag catattcgca
     2761 acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa
     2821 ttcacggtaa actccgggca gctcagattt cccgttattg ttaaagtcta atgcccggca
     2881 taacaaataa taaaaacccg catcttattc catcccgata taacgcttag ctcaccaatt
     2941 gccactgcct tttttccatc actggagaac taatcactga cattaacaac tctttcactg
     3001 cctgtgcctg tggcgataag ttcgctctgg cgggtaaatt taatgacaaa gagagactca
     3061 tggaaggcgt ggtaatgcgt gacatccacc catttactgc gccacataac gaacgcgcgg
     3121 ccgattcggg taatactgca acgcccatgc cgctggcaat cgctgcggta agcgtggcaa
     3181 tagactcaat ttcaccaatg acttttgccg tgagccgccg tagggaaaaa gcctcatcaa
     3241 cacgaagtct aacagcactg taatcactgg ggagaaagag gttcatttgc gcaatagcat
     3301 taacatcaac gctttgcccc gggcaatctt gagttcctac caggaaaaga tcttctttca
     3361 gcaaagcctg actggatacc ccagccacag gggaatgctc ataaatcacc gccatatcga
     3421 gttggtgatt tatcagtttt tcgttaagca ctgcaccgct attttcatga agatagataa
     3481 cgatctccgg gaattcagcg cgaaccgctt gtaataaggg catggtgata gatgacgcag
     3541 cggttcctgg cgcaaagcca atcgagactt gccccgataa tgcctgacca acgttatgca
     3601 ccgccagttg ggcctgctca cactgacgta aaatggcccg cgcatgggta tagagaattt
     3661 ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat caaaagttgt tgatttaact
     3721 caccttccag tgtggcaacc tgctggctga gcgctggttg tgcgatatgc aatacttcag
     3781 cagcctgagt caggctacca atatctacaa tttttacgaa gtatttcagg cgtctgaagt
     3841 tcatgttgcc tccggttttt gagaatcggc ccaagtgccg ccattactta caaccagact
     3901 gcaagatgct tgccagtttt attttggcgt tgatgtacaa gctaaccaac tgtcaaataa
     3961 gagattatga tagattcgtc attggctctt ttcaccagca gtcgcgttcc cctgccctat
     4021 aaaaggaggg tatgcaccac gatggttcat taccaataag attgaaatct catcactttg
     4081 ttgaaattgg cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     4141 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     4201 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     4261 gctttctagc aggatttttg ctttctaatt accaacgctc ataaaacatc tgtcttgaac
     4321 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattca
     4381 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     4441 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccagt
     4501 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgttc
     4561 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     4621 gacttacccg aagtccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     4681 caatataaat cgcgtacagc cccatgggat tatcgggccc cgcaggaaca aatgcgggca
     4741 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     4801 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     4861 cgataccaat aggaaagact tccacagtat tactgtctgg cgggtagtaa taaagacgca
     4921 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     4981 gaatggtgag ttgcgagcca gacttcggca agaaaacatc agcgcccgga ttcgcttcca
     5041 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     5101 gatcaggaac agttacagta agcgactgcc ccaccaaacg gctaccctct ggaggtaatg
     5161 gataagttac cgccaggcta gtatggctgg caaaaagcag agcaaatgag caaagaatat
     5221 ttacacgacg catcatgtcc ctttcctatg tcgcgaaagc tatccgttaa gtatagcttt
     5281 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     5341 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     5401 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     5461 agccccaaat gcgacagcgc tatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     5521 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     5581 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     5641 ccgaccaaat caaatccccc cacttttgcc agaacatcaa cgccatcctg cggatttggt
     5701 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     5761 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     5821 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     5881 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     5941 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     6001 agcccgggga taggctcagc actatcaata ccaacatcaa ctacgtggac gttagcgccc
     6061 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     6121 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg agcggcacac
     6181 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcaaccccgg cattcccgcc
     6241 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtca
     6301 atatgccgtt gtgcacgcga catagcggca ggatcgatag cagggatcgt attcagtaaa
     6361 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     6421 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     6481 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     6541 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     6601 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgggcataa cgatgacgat
     6661 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     6721 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     6781 tcaatgccag accgccatgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     6841 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtct aggtggaatc
     6901 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     6961 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     7021 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac ctgcgacgcg
     7081 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttactc atttgatttt
     7141 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
     7201 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     7261 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
     7321 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     7381 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attcaagcaa
     7441 cacaacctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     7501 cactgtgcgc cagtgctccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     7561 atcatcaagg atttgcgagg tagcgatata cagaacctgt gaagagtccc caataagcgc
     7621 ctctgcgtgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     7681 tcctgattca aatgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     7741 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     7801 caatccatta cgaaataaga tggcattacg gaatagccag actttctcgt gttaacgtcc
     7861 gacaaaatgc gtcattgaca gcggtttctg tggattgaca catttcattc agataatgaa
     7921 ttaatgctac tgccggaaca accagcaaac gggcattgtg ttctgaaatc caggctattg
     7981 attcaacgtc agcgacagat ctgcgctgta aaagaacttg taaaacaacg taatagaatt
     8041 atccggaatg gtggcgacta tgcactagaa caccacaatt tcgctctctc ggtaaggcac
     8101 tgtcacagca ttcaacagaa tgtgacttgc cagatttatt agcgccacca gatgtttaac
     8161 cgggtaacca cgacccagtc ggggatatat tccatcttct cgctgacatc atcaagaata
     8221 aaaaggttta tcacactcag agcaacgttc ttcagcaggc gggataattc ttcaacattt
     8281 agaagatgtg ttattaaagg catacaaact ggacgtattg tattttcttt tggtggttga
     8341 cctaaggtag cagtttatcc tgatgcgctg agatttctgt aatatctacg tcaacattct
     8401 cctcgaatag aaaatgcagc cccggcaagt gattcacatt ttttaacaaa acgttgttag
     8461 cgtgccattt tcagaagctc tccaagaaag cgaattcttt tctcaaattc tgcattctca
     8521 gacacagctt tctactccat ggtaatggcc caaatacgaa gttgctcagg atcattagtg
     8581 gtgctgagaa gttaaataac catgcggagt accactcgtc atcacgtatt tatatatcat
     8641 gaagctattg ttgtgttttg tacatcagta gatatattct gatatactcc ttttgctaga
     8701 cataaccttt cacctgcttg caaagcttct gtgttctgac attgccaaat tgttgcaatt
     8761 ctgtatccag ccttctttca gtcatagctt cgggccgcga taagactcac tgatctgacc
     8821 ctgattcctc ttgcagactt tatagaccaa ttaaaatgca gtttctgcag gtcaacgtct
     8881 gaccatcatt gtcatcactc tggccattag agtaaccttc tgcattcatc cttttgtaaa
     8941 aagtttatgt cagtatcagc aattaaccgg acctgatact gatatgagtc ttaccgcata
     9001 tacggtcaat ttcagcaatt aattacatta tccacgccaa agtgtttgtc atcgcaatga
     9061 tggtaccttc tttcagacac cattttttca actccgtttt ccacggaccg cactcttatg
     9121 tcaagagtgc ggtgatccta cccacgtaat atggacacag gcctaagcga ggttctggtt
     9181 ttcaaattgt tccggactga ggccgccaca ccaactgtgc cgccgccacc gattgtaatc
     9241 acattcgata taattaaaca ccgttgcccg cattatttcc cggctgataa agtgttctcc
     9301 atggatacat tccactttca gcgaatgaaa gaagctttcc acgcagatta tgccgcttca
     9361 gttgcgcctg ataatctgct gaacagtact ggcctccacg gtccgtgtga acgataacgt
     9421 tccggggcct cttacgccgc ca
//
LOCUS       DAZFRJ010000169         9300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000169 DAZFRJ010000000
VERSION     DAZFRJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.171"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..257)
                     /locus_tag="ACHKUN_003741"
     CDS             complement(<1..257)
                     /locus_tag="ACHKUN_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661926.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINKFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINLATSP"
     gene            781..2709
                     /gene="thrS"
                     /locus_tag="ACHKUN_003742"
     CDS             781..2709
                     /gene="thrS"
                     /locus_tag="ACHKUN_003742"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO6661927.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2713..3255
                     /gene="infC"
                     /locus_tag="ACHKUN_003743"
     CDS             2713..3255
                     /gene="infC"
                     /locus_tag="ACHKUN_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO6661928.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3352..3549
                     /gene="rpmI"
                     /locus_tag="ACHKUN_003744"
     CDS             3352..3549
                     /gene="rpmI"
                     /locus_tag="ACHKUN_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO6661929.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3602..3958
                     /gene="rplT"
                     /locus_tag="ACHKUN_003745"
     CDS             3602..3958
                     /gene="rplT"
                     /locus_tag="ACHKUN_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO6661930.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4075..4199
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4081..4125
                     /gene="pheM"
                     /locus_tag="ACHKUN_003746"
     CDS             4081..4125
                     /gene="pheM"
                     /locus_tag="ACHKUN_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO6661931.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4409..5392
                     /gene="pheS"
                     /locus_tag="ACHKUN_003747"
     CDS             4409..5392
                     /gene="pheS"
                     /locus_tag="ACHKUN_003747"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO6661932.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5407..7794
                     /gene="pheT"
                     /locus_tag="ACHKUN_003748"
     CDS             5407..7794
                     /gene="pheT"
                     /locus_tag="ACHKUN_003748"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO6661933.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7799..8098
                     /gene="ihfA"
                     /locus_tag="ACHKUN_003749"
     CDS             7799..8098
                     /gene="ihfA"
                     /locus_tag="ACHKUN_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO6661934.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(8400..8540)
                     /locus_tag="ACHKUN_003750"
     CDS             complement(8400..8540)
                     /locus_tag="ACHKUN_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6661935.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(8731..8991)
                     /locus_tag="ACHKUN_003751"
     CDS             complement(8731..8991)
                     /locus_tag="ACHKUN_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6661936.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASAVHSSEGIPWHELPASHRGNNQMSLPLPQN"
BASE COUNT         2411 a         2134 c         2444 g         2311 t
ORIGIN      
        1 atgggtgaag tggcaaggtt aatggtaaaa agggcagtgg aataacaatt ttgtgatatt
       61 tttcgacagt ttgcggtaaa ttcctgagta acacagtatg acagcatcga acttaagtca
      121 gaggtatcaa gtagggtgat gagctggcgc gcaaatttat taataggctc tgatgaaggt
      181 gttggtatgt cgctatcgat gtgcaatcta tgaggagact tataatgagt tctgataatg
      241 atatcgtttt gactcataat ctattcgcct tatatatatc tggaggtttt gaggatgatg
      301 attaattatt atcgccagaa cgaaatgtgc acgaaaaatt gttataagaa ttgtgtcact
      361 tttatcattg tgatatgacc acttcgtcat taatctcatg agcgtttaaa atactaccgc
      421 caaatcacgc aacgaacttt gctggattca gtttatgctg ctgtaaatcc gctcgcagta
      481 aacctttcag acgcaccgtg atgttatcag tggttcttta agcgttttgc tggtgtactc
      541 actacaaacg aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc tccctttttt
      601 tagtgttaac atcgctcaac cgggtggaag gtgagaggtt aatgtctttc atttgtgtac
      661 ctgattgcga accaatttag catttgttgg ctagatggtt tcgcaatgaa ctgttaataa
      721 acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata aggatataaa
      781 atgcctgtta taactcttcc tgatggcagc caacgccatt acgaccacgc tgtaagcccc
      841 atggatgttg cgctggacat tggtccaggt ctggcgaaag cctgtatcgc agggcgcgtt
      901 aatggcgaac tggttgatgc ttgcgatctg attgaaaacg acgcacaact gtccatcatt
      961 accgccaaag acgaagacgg tctggagatc attcgtcact cctgtgcgca cctgttaggg
     1021 cacgcgatta aacaactttg gccgcatacc aaaatggcaa tcggcccggt tattgacaac
     1081 ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt cgaagcactc
     1141 gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa gaaagtcagc
     1201 tggcacgaag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt ctccattctt
     1261 gacgaaaaca tcgcccatga tgacaagcca ggtctgtact tccatgaaga atatgtcgat
     1321 atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc atcatttcaa actaatgaaa
     1381 acggcagggg cttactggcg tggcgacagc aacaacaaaa tgttgcaacg tatttacggt
     1441 acggcgtggg cagacaaaaa agcgcttaac gcttacctgc agcgcctgga agaagccgcg
     1501 aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca ggaagaagcg
     1561 ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact agaagtgttt
     1621 gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt catgatggac
     1681 cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca aagatgcaat gttcaccacg
     1741 tcttctgaga accgtgaata ctgcattaag ccgatgaact gcccgggtca cgtacaaatt
     1801 ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga gtttggtagc
     1861 tgccaccgta atgagccgtc aggttcgctg catggcctga tgcgcgtgcg tggatttacc
     1921 caggatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt taacggatgt
     1981 atccgtttag tctatgatat gtacagcact tttggcttcg agaagatcgt cgtcaaactc
     2041 tccactcgtc ctgaaaaacg tattggcagt gatgaaatgt gggatcgtgc tgaggcggac
     2101 ctggcggttg cgctggaaga aaacaacatc ccgtttgaat atcaactggg tgaaggcgct
     2161 ttctacggtc cgaaaattga atttaccctg tatgactgcc tcgatcgtgc atggcagtgc
     2221 ggtacagtac agctggactt ctctttgcct tctcgtctga gcgcttctta tgtaggcgaa
     2281 gacaatgaac gtaaagtacc ggtaatgatt caccgcgcaa ttctgggatc gatggaacgt
     2341 ttcatcggta tcctgaccga agaattcgct ggtttcttcc cgacctggct tgcgccagtt
     2401 caggttgtta tcatgaatat taccgattca cagtctgaat acgttaacga attgacgcaa
     2461 aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa gattggcttt
     2521 aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg tgataaagag
     2581 gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta aagacctggg aagcatggac
     2641 gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca gccgcagtct taaacaattg
     2701 gaggaataag gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa ccgtatcaat
     2761 ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagca gcttggtatt
     2821 gtgagtctga gagaagctct ggagaaagca gaagaagccg gagtagactt agtcgagatc
     2881 agccctaacg ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt cctctatgaa
     2941 aagagcaagt cttctaagga acagaagaaa aagcaaaaag ttatccaggt taaggaaatt
     3001 aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa aactccgcag cctgattcgc
     3061 tttctcgaag agggtgataa agccaaaatc acgctgcgtt tccgcggtcg tgagatggcg
     3121 caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag acgatttgca agaactggca
     3181 gtggtcgaat ccttcccaac gaagatcgaa ggccgtcaga tgatcatggt gctcgctcct
     3241 aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg agccttcggg cttcacaggc
     3301 tttgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa aatgccaaaa
     3361 attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg tggttttaag
     3421 cacaagcacg ctaacctgcg tcacattctg accaaaaaag cgaccaaacg taaacgtcac
     3481 ctgcgtccga aagccatggt ttccaaaggc gatctgggcc tggtaatcgc gtgcctgccg
     3541 tacgcataag ccgttaacgt ttttaacttt ttaattagaa tatagataca ggagagcaca
     3601 tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa ttttgaaaca
     3661 agctaaaggc tactacggtg cgcgttctcg cgtataccgc gttgccttcc aggctgttat
     3721 caaagctggt cagtatgctt accgtgaccg tcgtcaacgt aagcgtcagt tccgtcaact
     3781 gtggattgcg cgtatcaacg cagcagcacg tcagaacggt atttcttaca gcaaattcat
     3841 caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg atatcgcagt
     3901 attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg aaagcagctc tggcataagc
     3961 cagttgaaag agggagctag tctccctctt ttcgtttcaa cgccatcaaa acattgactt
     4021 ttatcgccgt agccttttca ataaaggtct tttgaagagt accaaaaggt aacgcaagca
     4081 atgaatgctg ctattttccg cttctttttt tactttagca cctgaatcca ggaggctagc
     4141 gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc cagtggaggc tttttttgta
     4201 tgcgcgtttt gaaaaataaa aagcctgtct catcaggtgt aattgtcgca gccagtctgg
     4261 acacggccag cgcggagaaa ccggagcgta tttaagtacg tgagaatttc gagcactgcc
     4321 cgggaccaaa atggcaagta aaatagcctg atgggatagg ctctaagtcc aacgaaccag
     4381 tgtcaccact gacacaatga ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc
     4441 gaaggcggcc attagccagg cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata
     4501 tttgggtaaa aaagggcact taacccttca gatgacgacc ctgcgtgagc tgccgccaga
     4561 agagcgtccg gcagctggtg cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct
     4621 gaatgcgcgt aaagcggaac tggaaagcgc tgcactgaat gcgcgtctgg cggcggaaac
     4681 gattgatgtc tctctgccag gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg
     4741 tactatcgac cgtatcgaaa gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc
     4801 ggaaatcgaa gacgattatc ataacttcga tgctctgaac attcctggtc accacccggc
     4861 gcgcgctgac cacgacactt tctggtttga cactacccgc ctgctgcgta cccagacctc
     4921 tggcgtacag atccgcacca tgaaagccca gcagccaccg attcgtatca ttgcgcctgg
     4981 ccgcgtttat cgtaacgact acgaccagac tcacacgccg atgttccatc agatggaagg
     5041 tctgattgtt gataccaaca tcagctttac caacctgaaa ggcactctgc acgatttcct
     5101 gcgtaacttc tttgaggaag atttgcagat tcgcttccgt ccttcctact tcccgttcac
     5161 cgagccttct gcagaagttg acgtcatggg taaaaacggt aaatggctgg aagtgctggg
     5221 ttgcgggatg gtgcatccga acgtgctgcg taacgttggc atcgacccgg aagtttactc
     5281 tggtttcgcc ttcgggatgg ggatggagcg tctgactatg ttgcgttacg gcgtcaccga
     5341 cctgcgttca ttcttcgaaa atgatctgcg tttcctcaaa cagtttaaat aaggcaggaa
     5401 tagattatga aattcagtga actgtggtta cgcgaatggg tgaacccggc gattgatagc
     5461 gatgcgctgg cgaatcaaat cactatggcg ggcctggaag ttgacggtgt agaaccggtt
     5521 gccggtagct tccacggcgt ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac
     5581 gctgacaaac tgcgtgtgac aaaagtgaat gttggcggcg atcgcctgct ggacatcgtc
     5641 tgcggtgcgc caaactgccg tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt
     5701 ctgccgggtg atttcaaaat taaagcggcg aaactgcgtg gtgaaccgtc tgaagggatg
     5761 ctgtgctcct tttctgagct gggaatttct gacgatcata gcggcattat cgagctgcct
     5821 gcggatgcgc cgattggcac cgacatccgt gaatacctga aacttgatga caacaccatc
     5881 gaaatcagcg tgacgccaaa ccgtgccgac tgtttaggta tcattggtgt tgcgcgtgac
     5941 gttgccgtgc tgaatcagct gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc
     6001 atcgacgaca cgctgccgat tacagtcgaa gcgccggaag cctgcccgcg ttatcttggc
     6061 cgtgtggtaa aaggcattaa cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg
     6121 cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg tcaccaacta tgtactgctc
     6181 gaactgggcc agccgatgca cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg
     6241 cggatggcga aagagggcga aacgttggta ctgcttgatg gcactgaagc gaagctgaat
     6301 gctgacactc tggtcatcgc cgaccacaac aaggcgctgg cgatgggcgg catctttggt
     6361 ggcgaacact ctggcgtgaa tgacgaaacg caaaacgtct tgctggaatg cgctttcttc
     6421 agcccgctgt ctatcaccgg tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac
     6481 cgttatgagc gtggcgttga tccggcactg cagcacaaag cgatggaacg tgcgacccgt
     6541 ctgctgatcg acatctgcgg tggtgaggct ggtccggtaa ttgatatcac caacgaagca
     6601 acgctgccga agcgtgcaac catcacttta cgtcgtagca aactggatcg cctgatcggc
     6661 catcatattg cggatgagca ggtaactgac attctgcgtc gtctcggctg cgaagtgacc
     6721 gaaggcaaag acgagtggca ggcagttgcg ccgagctggc gtttcgatat ggagattgaa
     6781 gaagatctgg tcgaagaagt cgcgcgtgtt tacggctaca acaacatccc ggatgagccg
     6841 gtacaggcaa gcctgattat gggtactcac cgtgaagctg acctgtcgct caagcgcgtg
     6901 aaaacgctgc tcaacgacaa aggctatcag gaagtgatca cctacagctt cgttgatccg
     6961 aaagtgcagc agatgatcca tccaggcgtt gaagccttac tgctgccaag cccgatctct
     7021 gttgaaatgt cagcaatgcg tctttctctg tggaccggcc tgctggcaac cgtggtgtac
     7081 aaccagaacc gtcagcagaa ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca
     7141 gatactcagg caccgttggg cattcgtcag gatctgatgt tagccggtgt gatttgcggt
     7201 aaccgttacg aagagcactg gaacctggca aaagagaccg ttgatttcta tgatttgaaa
     7261 ggcgatcttg aatccgttct cgacctgacc ggtaaactga atgaggttga gttccgtgca
     7321 gaagcgaatc cggcactgca tccggggcaa tccgcagcga tttatctgaa aggtgaacgt
     7381 attggttttg ttggggttgt tcatcctgaa ctggaacgta aactggatct taacggtcgc
     7441 actctggtgt tcgaactgga gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc
     7501 gagatttctc gcttcccggc gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt
     7561 cccgcagcgg atattttatc cgaatgtaag aaagttggcg taaatcaggt agttggcgta
     7621 aacttatttg acgtgtaccg cggtaagggt gttgcggagg ggtataagag cctcgccata
     7681 agcctgatcc tgcaagatac cagccgtaca ctcgaagaag aggagattgc cgctaccgtc
     7741 gccaaatgtg tagaggcatt aaaagagcga ttccaggcat cattgaggga ttgaacctat
     7801 ggcgcttaca aaagctgaaa tgtcagaata tctgtttgat aagcttgggc ttagcaagcg
     7861 ggatgccaaa gaactggttg aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg
     7921 cgaacaggtg aaactctctg gttttggtaa cttcgatctg cgtgataaga atcaacgccc
     7981 gggacgtaac ccgaaaacgg gcgaggatat tcccattaca gcacggcgcg tggtgacctt
     8041 cagacccggg cagaagttaa aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc
     8101 tgatctaact aaaaaggccg ctctgcggcc ttttttcttt caataaccaa ttgatttata
     8161 atgttttata tctcttcgtt ccacgatgtg tccacattta gtggggggag gggagtaagc
     8221 ccgctacatt gtgggctttg tcttatagtc tgatcactgt ttcaagtcgg tgaggacacc
     8281 aaaaagaaaa cccgcagttt ttatgctgcg ggtttgtttc atgtctgtga gatagagtgc
     8341 cttatcgacc ttaccctggc aaccgattga cgggggattg ctcccccgtc gcggtttcct
     8401 tactgcttac actgtaagaa cgccgcaaac tccgctcccc agaagctcat ccgtatttcg
     8461 cacagtgaac cgtgcagcat ccagatgatg aggattgccg tcacgcagaa cgtgatggcc
     8521 gtaagcgatt tttgcgacat agcacttgct ccttttccgg agaggcgcta acctttcact
     8581 tgtcaaggta atgcggttag ggcctcggtt aaacagagat gttttccggg gcctttccac
     8641 atctggcctt cgggtattcc ctccgaccat cagccgaaag gcacccgcgc gtaatctatc
     8701 gcttttttgt taatccggca attctgcctg ttagttctga ggtaaaggca aactcatctg
     8761 attgtttccc ctgtgtgaag ctggcagctc atgccacggg ataccttctg aagagtgaac
     8821 ggcggaggcg tgttttgatg tgaatttatg gaaggcttcc agtgttgaga agcatacgcc
     8881 gcattccagg ttgttacact ggtaatactt ttgccgcacg gtgtttgaat cattttccgg
     8941 acgacttgtg cggatacggg cagatgcgcc acaaagcgga caacggaaca tagagacctc
     9001 ccttaacgtg gtgcttctgt tattttaagt tctactagct ccgattccaa ctgactaaac
     9061 cgctttgagt tgaggggctg acattcttat tattcgctta tcaaagaata gctagtcaaa
     9121 cctgatacat taaaatcagc acgttatttt gattgtgtga tatcataaca ttatgttatt
     9181 aatgcgttaa attgggatat ctaattcaga ttgtgaggta tttatgggaa agaatagtca
     9241 acgtaggcga gataataaaa ttaagcgaga aaatgagaaa aaaagaaact tagcggcaaa
//
LOCUS       DAZFRJ010000170         9175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000170 DAZFRJ010000000
VERSION     DAZFRJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.172"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            224..1855
                     /gene="oppA"
                     /locus_tag="ACHKUN_003752"
     CDS             224..1855
                     /gene="oppA"
                     /locus_tag="ACHKUN_003752"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO6661937.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1941..2861
                     /gene="oppB"
                     /locus_tag="ACHKUN_003753"
     CDS             1941..2861
                     /gene="oppB"
                     /locus_tag="ACHKUN_003753"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO6661938.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2876..3784
                     /gene="oppC"
                     /locus_tag="ACHKUN_003754"
     CDS             2876..3784
                     /gene="oppC"
                     /locus_tag="ACHKUN_003754"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO6661939.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3796..4809
                     /gene="oppD"
                     /locus_tag="ACHKUN_003755"
     CDS             3796..4809
                     /gene="oppD"
                     /locus_tag="ACHKUN_003755"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO6661940.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4806..5810
                     /gene="oppF"
                     /locus_tag="ACHKUN_003756"
     CDS             4806..5810
                     /gene="oppF"
                     /locus_tag="ACHKUN_003756"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO6661941.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5863..6192)
                     /locus_tag="ACHKUN_003757"
     CDS             complement(5863..6192)
                     /locus_tag="ACHKUN_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO6661942.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6227..7687)
                     /gene="cls"
                     /locus_tag="ACHKUN_003758"
     CDS             complement(6227..7687)
                     /gene="cls"
                     /locus_tag="ACHKUN_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO6661943.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7830..8003
                     /locus_tag="ACHKUN_003759"
     CDS             7830..8003
                     /locus_tag="ACHKUN_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO6661944.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8058..>9175)
                     /gene="kch"
                     /locus_tag="ACHKUN_003760"
     CDS             complement(8058..>9175)
                     /gene="kch"
                     /locus_tag="ACHKUN_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO6661945.1"
                     /translation="DIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWA
                     ISIILLLIALIYTLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFT
                     TLLFYSTYGALYLSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVI
                     ISGITVFATSMTSIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQ
                     RGQNVTVISNLPEDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDND
                     ADNAFVVLSAKDMSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGE
                     EINNDMLVSMLLNSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2368 a         2170 c         2301 g         2336 t
ORIGIN      
        1 taaagagcgt cgcgaaagaa taatgtgtct cgacagggga gacacagtac gaatcgacat
       61 aaggtgatcg tctgaatcac cagaataaat aaagtcggtg atagtaatac gtaacgataa
      121 agtaacctga cagcagaaag tctccgagcc tgtgcagggt cccaatccgg gattacacat
      181 gctggttaat accagtaatt ataatgaggg agtccaaaaa acaatgacca acatcaccaa
      241 gagaagttta gtagcagctg gcgttctggc tgcgctaatg gcagggaatg tcgcgctggc
      301 agctgatgta cccgcaggcg tcacactggc ggaaaaacaa acactggtac gtaacaatgg
      361 ttcagaagtt cagtcattag atccgcacaa aattgaaggt gttccggagt ctaatatcag
      421 ccgagacctg tttgaaggct tactggtcag cgatcttgac ggtcatccag cacctggcgt
      481 cgctgaatcc tgggataata aagacgcgaa agtctggacc ttccatttgc gtaaagatgc
      541 gaaatggtct gatggcacgc cagtcacagc acaagacttt gtgtatagct ggcaacgttc
      601 tgttgatccg aacactgctt ctccgtatgc cagttatctg caatatgggc atatcgccgg
      661 tattgatgaa attcttgaag ggaaaaaacc gattaccgat ctcggcgtga aagctattga
      721 tgatcacaca ttagaagtca ccttaagtga acccgttccg tacttctata aattacttgt
      781 tcacccatca acttcaccgg tgccaaaagc cgctatcgag aaattcggcg aaaaatggac
      841 ccagcctggt aatatcgtca ccaacggtgc ctatacctta aaagattggg tcgtaaacga
      901 acgaatcgtt cttgaacgca gcccgaccta ctggaacaac gcgaaaaccg ttattaacca
      961 ggtaacctat ttgcctattg cttctgaagt taccgatgtc aaccgctacc gtagtggtga
     1021 aatcgacatg acttataaca acatgccgat cgaattgttc cagaagctga aaaaagagat
     1081 cccggacgaa gttcacgttg atccatacct gtgcacttac tattacgaaa ttaacaacca
     1141 gaaaccgcca ttcaacgatg tgcgtgtgcg taccgcactg aaactaggta tggaccgcga
     1201 tatcattgtt aataaagtga aagcgcaggg cgacatgccc gcctatggtt acactccacc
     1261 gtatactgat ggcgcaaaat tgactcagcc ggaatggttt ggctggagcc aggaaaaacg
     1321 taacgaagaa gcgaaaaaac tgctggctga agcgggttat accgcagaca aaccgttgac
     1381 catcaacctg ttgtataaca cctccgatct gcataaaaag ctggcgattg ctgcctcttc
     1441 attgtggaag aaaaacattg gtgtaaacgt caaactggtt aaccaggagt ggaaaacgtt
     1501 cctcgacacc cgtcaccagg gtacttttga tgtggcccgt gcaggctggt gtgctgacta
     1561 caacgaacca acttccttcc tgaacaccat gctttcgaac agctcgatga ataccgcgca
     1621 ttataagagc ccggcctttg acagcattat ggcggaaacg ctgaaagtga ctgacgaggc
     1681 gcagcgcaca gctctgtaca ctaaagcaga acaacagctg gataaggatt cggccattgt
     1741 tcctgtttat tactacgtga atgcgcgtct ggtgaaaccg tgggttggtg gctataccgg
     1801 caaagatccg ctggataata cctatacccg gaatatgtac attgtgaagc actaatggca
     1861 atacgtgggg caggagtgtc ctgctccacg gtgtctgatt tttatcgcat tacagaaggc
     1921 acaggccaga aggtagggca atgttaaaat ttattctacg tcgctgtctg gaagcgattc
     1981 cgacgctatt tattcttatt actatttcgt tctttatgat gcgcctcgcg ccgggaagcc
     2041 cttttaccgg cgaacgtact ttaccgccag aagtgatggc caatatcgaa gagaaatatc
     2101 atcttaatga tccgatcatg acacagtatt tcagctacct gaaacaactg gcgcacggtg
     2161 atttcggtcc atcgtttaaa tataaagatt attcggtcaa tgacttggtt gcatccagtt
     2221 ttcccgtttc tgccaaactg ggagccgcag catttttcct tgcggtaata ctgggggtta
     2281 gtgctggcgt tattgccgca ttaaaacaaa acaccaaatg ggactatacc gtgatggggc
     2341 tggcaatgac cggggttgtt atccccagtt ttgtggttgc accattatta gtcatgatat
     2401 ttgcgatcat tttgcattgg ctgccgggcg gtggctggaa tggtggggcg cttaaattca
     2461 tgatattgcc gatggtggcg ttgtcactcg cttatatcgc cagtattgcg cgtattaccc
     2521 gtggctctat gattgaagta ttacactcca attttattcg tactgcccgg gcgaaagggt
     2581 tacctatgcg gcggatcatt ttacgtcacg cattaaaacc tgctctgtta cccgtgctct
     2641 cttatatggg ccccgcattt gtcggcatta ttaccggttc tatggttatc gaaaccattt
     2701 atggtttgcc ggggattgga caactgttcg ttaatggcgc attgaaccgt gactattcct
     2761 tagtgttaag cctgaccatc ctggttggtg ctttaaccat tttgtttaat gccattgtcg
     2821 atgtgctata tgcggttatc gacccgaaaa tccgttactg atactggagc tcgcgatgat
     2881 gttaagtaag aaaaacagcg agacgctgga aaatttcagt gaaaagctgg aggtcgaagg
     2941 gcgcagcttg tggcaggacg cacgtcgacg ttttatgcat aaccgtgcgg cggttgccag
     3001 tctgatagtg ctggtgctga tcgcgttatt tgtaatcctg gcaccgatgc tttcccagtt
     3061 tgcctatgac gatactgact gggcgatgat gtccagcgcc ccggatatgg agtccggtca
     3121 ctactttggt actgactcat ccggtcgcga cctgcttgtg cgcgttgcga ttggcgggcg
     3181 tatctcactc atggtcggtg ttgctgcggc actggtggct gtggtcgtcg ggacacttta
     3241 tggttcgctt tccggttatc tgggcggtaa agtggattcg gtaatgatgc gtctgctgga
     3301 aatcctcaac tccttcccat tcatgttctt cgtcattttg ctggtgacct ttttcggtca
     3361 aaacatcctg ctgattttcg tggcgatagg catggtttcc tggctggata tggctcgtat
     3421 tgtgcgtggg caaaccctga gtctgaagcg caaagagttt attgaggcgg cacaagttgg
     3481 cggtgtatcg acatcgggca ttgttattcg ccacattgtg ccgaatgtac tcggtgtggt
     3541 ggtggtctac gcatcacttc tggtgcccag catgatcctc tttgaatctt tccttagctt
     3601 cctggggttg ggtacgcaag agccgttaag cagctggggt gcattgctga gtgatggcgc
     3661 gaactcgatg gaagtctctc catggttatt gttgttccca gcgggattcc tcgtggtgac
     3721 gctgttttgt ttcaacttta tcggcgatgg cttgcgtgat gccctcgacc cgaaagatcg
     3781 ttaaggagtg cagccatgag cgtaattgaa actgcaactg tgccgctcgc acaacaacag
     3841 gctgacgcac tgctgaacgt gaaagatttg cgtgtcacct ttagtacccc ggacggcgac
     3901 gtcacggcgg tcaatgattt gaatttttcc ctacgtgccg gagaaacgct gggtattgta
     3961 ggtgagtctg gttcgggtaa atcgcaaact gcatttgcgt tgatgggcct gttggctgcc
     4021 aacggacgta ttggcggatc ggcaaccttc aatgggcgtg aaatcctcaa tttgccagag
     4081 catgaactca ataaactgcg cgctgaacaa atctcaatga tttttcagga cccaatgact
     4141 tcgttgaatc cctatatgcg cgtcggtgag cagttaatgg aagtgctgat gctgcataag
     4201 aacatgagca aagctgaggc gtttgaagag tcggtgcgga tgctcgatgc ggtaaaaatg
     4261 ccggaagcgc gtaaacgcat gaaaatgtac ccgcacgaat tttctggcgg catgcgtcag
     4321 cgagtcatga ttgcgatggc attgctatgt cgacctaagc tactgattgc ggatgaaccc
     4381 actactgcgc tggacgtcac cgtacaggcg cagatcatga cgctattaaa tgaactgaag
     4441 cgggaattta ataccgccat cattatgatc acccacgatc ttggcgtggt ggcggggatc
     4501 tgtgacaaag tgctggtaat gtacgccggg cgcacgatgg aatatggcaa tgcgcgcgat
     4561 gtcttttatc aacccgttca tccttattct atcggtttgc tcaacgcggt gccgcgtctt
     4621 gatgcggaag gtgaaacaat gttgaccatc ccgggtaatc cgccaaactt gctgcgatta
     4681 ccgaaaggtt gcccgttcca gccacgttgt ccacatgcga tggaaatttg tagtagcgca
     4741 ccgccgctgg aagagtttac gcctggccgt ctgcgtgctt gctttaaacc ggtggaggaa
     4801 ctgttatgaa tgctgtaact gaaggaagaa aagtcctcct tgaaatcgcc gatcttaaag
     4861 tgcactttga aatcaaagat ggcaaacagt ggttctggca accgccgaaa acgctcaaag
     4921 ccgtcgatgg tgtcactctt cgcctgtatg aaggggaaac attaggtgtg gtaggggaat
     4981 cgggatgcgg taagtccacc tttgctcgcg ccatcatcgg tttggtcaag gcgaccgacg
     5041 gtcatgttgc ctggttaggt aaagagttgc tgggcatgaa gcccgatgaa tggcgtgccg
     5101 ttcgcagtga tattcagatg attttccagg atccgttggc atcgctaaac ccgcgtatga
     5161 ccatcggcga gatcatcgct gaaccactgc gtacttatca tccgaaaatg tcacgccagg
     5221 aagttcgcga gcgcgtgaag gcgatgatgc tgaaagtcgg gttattgcct aacctgatta
     5281 accgctatcc gcatgagttc tccggtgggc agtgccagcg tatcgggatt gctcgtgctc
     5341 ttattcttga accgaagctg attatctgcg atgagccggt ttcggcgctg gacgtgtcaa
     5401 ttcaggcgca ggtggtcaac ctgctccagc agctgcaacg tgagatggga ttgtcattaa
     5461 tttttatcgc tcatgacctg gccgtggtaa aacacatttc cgatcgtgtg ttggtgatgt
     5521 atctcggcca tgcggtagaa ctggggacct atgatgaggt ctaccacaat ccactacatc
     5581 cttacaccag ggcattgatg tcggcagtcc ccatacctga tccggatctg gagaagaaca
     5641 aaaccatcca gttactggaa ggggaattac cgtcgccgat caacccgcct tccggttgtg
     5701 ttttccgtac ccgttgcccg attgccggtc cggagtgcgc caaaacacgt cctgttctgg
     5761 aggggagttt cagacacgcc gtttcttgcc tgaaagtcga tccgctttaa acacaataag
     5821 ggctgacagt tgtcagccct ttttcacgct aaaagcgatt atttattccc gccagatgat
     5881 atggcaaagt ttgtgatctt tttcgcggca taacagaatg cgggcgaaaa catcgttgat
     5941 ttcaccatct tcactgtccg ccagaccaat caccacttcg gcaaaaaagt ctgggttcaa
     6001 atcgaaatca acatgttcct gccagtcttc cgccggatcg aataactccg cgccgccgcg
     6061 ctcttcaaac tgaagattga acagcagaac atcagctgga tcgagattgt cggcagccag
     6121 ttcgagaaaa atatcgtagg cctgctcaag cgtttcatct tcagtcaggc gattgtttag
     6181 atccatatcc atagtcacta cctgtttaac ctctgttggc gacgttttac agcaacggac
     6241 tgaagaagta aaacagtcgc tcggcgacac gttgccataa tggacgtttt agccataaac
     6301 gggcatcgag cagacgtgaa cgcgaaatat aatcgtcctg aacggcggca aggtctgcac
     6361 caaaaccttt atcgtcgatt gccagggtaa tctcgaaatt cagccacaga ctccgcatat
     6421 caaggttaac ggtgccaacc agacttagtt cgccatcgac cagcacgctc ttggtatgca
     6481 gtaacccgcc ttcaaactga taaattttaa ccccagcagc aagcagttcc gtaaagaatg
     6541 cgcgactggc ccagccgacc agcatcgagt cattttttcg cggaaggata atactgacat
     6601 ccaccccgcg ctgcgccgcc gtgcaaatcg catgaagtaa atcatcgctt ggcacaaagt
     6661 agggcgtggt catgatcaaa tattcacgcg ccgaataagc cgcagtcaat aatgcctggt
     6721 gaatgagatc ttccggaaag ccggggccag aagcaattgt gtgaatggtg tgaccgctgg
     6781 cctgttcaaa cggcataata ttgacatctg gtggtggcgg cagaatacgt tttccggttt
     6841 caatctccca gtcgcaggaa taaataatcc ccatcgcggt ggcgatgggg ccttccatac
     6901 gcgccatcag atcaatccat tgccctacgc ccgcatcttg tttgaagtag cgaggatcga
     6961 ccatattcat gctgccggtg tacgcgatgt aattatcgat catgatcatc ttgcgatgtt
     7021 ggcgcaggtc catacggcgt aaaaacacac gcatcagatt gacctttaag gcttcgacca
     7081 cttcaatacc ggcattacgc attagctcgg gccacgggct gcggaaaaaa gccacactcc
     7141 cggcggagtc gagcatcaat cggcaatgaa taccacgccg tgcagccgcc attaatgatt
     7201 cagccacctg gtccgccatg ccgccgggct gccagatata aaacaccatc tcaatattat
     7261 ggcgcgcgag ctggatgtcg cggattaacg cctgcatcac atcatctgac tcggtcatca
     7321 gttgtagctg attccctttg accccagcga tcccctgacg acgctcgcaa agcttgaata
     7381 atggcgcagc gacactgcta ttttcttcgg cgaagatatg cttacaggct ttaaggtcgt
     7441 taagccattt tgcggtggaa ggccacatcg ctctggcgcg ctcagcgcgg cgtttgccta
     7501 aatggagctc gccaacggca agataggcaa taattccgac taacggcaga atgtaaataa
     7561 tcaacagcca ggccatcgcg gagggaactg cgcgtcgttt cattagaatg cgtaaagtta
     7621 cgcctgcaat gagcaaccag tatcccagaa tggccaacca actcaccaac gtataaacgg
     7681 ttgtcataga ttagaaatcc ttttgaaagc gcattgttat gagtttacgc atatggattc
     7741 atctggcaaa taaaaacgcg ggaaaagcac tggtcagcgt cgggggtggg ggtataatga
     7801 ccattctgtt attgcataga gtagttaaca tgaagcggag tagaacggaa gtggggcgct
     7861 ggcgcatgca gcgtcaggct agccgacgta aatcgcgttg gcttgagggg caatcgcgcc
     7921 gaaatatgcg tatccacagc atcaggaagt gcattctaaa caaacagcgt aactcgttat
     7981 tgtttgcgat ctacaatatc taaatatact gggcaccatt gcggtgccca ataagtttac
     8041 tctttcgtct ttaaactcta tttttgcact gattctttac tgtcagcttt cgtttcttgt
     8101 tcatcgttat cgctgaaaat gccatgaccg gagttcaaca acattgaaac gagcatatca
     8161 ttattaatct cttcaccatt taaaactcgc gccagaattt cgctgccaaa cagttgcggt
     8221 gagagaatga tatccggatg taccatctta atcttattca ggtttttgct atcactgacg
     8281 gcgagaactg ttttgacatc actgctcata tcttttgccg agagtacaac aaacgcgtta
     8341 tctgcatcgt tatcactcag cgccagaatg gcccggcaac gatcgattcc cgctttcttt
     8401 aatactgaac tgtcattact gtcaccgggg ataacatcag cgttatcgcc taagcgttgc
     8461 tcaagttgct tgatatcatc ttcaggcaag ttgctgataa ccgttacgtt ttgtccgcgt
     8521 tgattcagtt gcaggatcgt attgatggcg agaatcgaat gtccgcaaac aataaaatga
     8581 tctttacgat gcattgtatg attgtttcct tttacaagtt tgttgaatcc cccgcggata
     8641 agcggaccaa aaattgaggt catagatgtg gcaaaaacgg taatgccgga aataatgacc
     8701 gaaatagtga acaatcgtgc tgattcagaa acagggacaa tatcgccata gccgacggtt
     8761 gacatggttt ctatcgaaaa atagaacgcg gtcatcaaac tttctattcg cggattaaaa
     8821 ccttcgctta aataaagcgc accgtaggtt gagtaaaaca gtaatgtcgt gaaactaata
     8881 aatgcaaaaa ttgtcccggc tgcggcgcta ctgtgggaga agtctttgcg cagtatcagc
     8941 aaaaacacca gcgtaaaaat gcaaaatcca atactaaatt tcagccaggg ataaaaatgc
     9001 agggtgtaaa ttagcgctat caacaacaaa atgatactga ttgcccatgc gagctttgcc
     9061 cggaacagta agccaatgga gttcaggaca agaaagacgc cgagcataaa tagtggggcg
     9121 tttgccagca aggagagatc caactctgaa aatgctttga tatggaaaat atcaa
//
LOCUS       DAZFRJ010000171         9136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000171 DAZFRJ010000000
VERSION     DAZFRJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.173"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            305..457
                     /gene="hokE"
                     /locus_tag="ACHKUN_003761"
     CDS             305..457
                     /gene="hokE"
                     /locus_tag="ACHKUN_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6661946.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(579..1199)
                     /gene="entD"
                     /locus_tag="ACHKUN_003762"
     CDS             complement(579..1199)
                     /gene="entD"
                     /locus_tag="ACHKUN_003762"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO6661947.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1236..1334)
                     /locus_tag="ACHKUN_003763"
                     /pseudo
     CDS             complement(1236..1334)
                     /locus_tag="ACHKUN_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="ACHKUN_003764"
     CDS             complement(1374..3614)
                     /gene="fepA"
                     /locus_tag="ACHKUN_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO6661948.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3857..5058
                     /gene="fes"
                     /locus_tag="ACHKUN_003765"
                     /pseudo
     CDS             3857..5058
                     /gene="fes"
                     /locus_tag="ACHKUN_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            5061..5279
                     /gene="ybdZ"
                     /locus_tag="ACHKUN_003766"
     CDS             5061..5279
                     /gene="ybdZ"
                     /locus_tag="ACHKUN_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO6661949.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            5276..>9136
                     /gene="entF"
                     /locus_tag="ACHKUN_003767"
     CDS             5276..>9136
                     /gene="entF"
                     /locus_tag="ACHKUN_003767"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO6661950.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPII"
BASE COUNT         1971 a         2426 c         2535 g         2204 t
ORIGIN      
        1 atggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgatttcg
       61 ttttaagagc aatgatgagt tttgccgaca gaagtccgaa acaggattca ctgtataaat
      121 aaacagatat tttgttgagg aagggtaaga taacggcggg tgcctgaagc tttccggttt
      181 caggttaact ctgaggtctg gaaagatgaa gccccaggag atatttctat caatcctggg
      241 gctgccactc cgagcctcaa caactcggat ggtagtccct tcttcgcatg gaggcaatat
      301 aaacatgctg acgaaatatg cccttgtggc agtaatagtg ctgtgtttga cggtactggg
      361 atttacgctt ctggtcggag actcgctgtg tgagttcacc gtaaaggaac gtaatattga
      421 gtttaaggct gttctcgctt acgaaccgaa gaagtagccg ttgtgcgggg agcaatcccc
      481 gcatatccgg ttgtcaggtc agagtggtaa ggcacctgct ttacactttc gcccgcggtc
      541 agtgatggct gcgggcgaat cgtaccagat gttgtcaatt aatcgtgttg gcacagagtt
      601 atgactattt tttcttttat ctgccagtgc acagcaaaca tctcattctc acgatgaatg
      661 atgacctgct gtttattcca gctaattatc tgatagtcca gaaaacctgc atcagtttgg
      721 atctcacttg ccttaaatgc gctctctttg gcggaaaatg ccagtgtcag cgccagagaa
      781 aaggctaaac cgcagtctgc gagtcgctcg tgttctgctg gtgtaataat gttgtctgtc
      841 aattcggtcg ctgtttgcgc agaaaaaatt tcttcgatat caacgccaat cggttgacgg
      901 gaaaccacgg ccaatgccgt agccccgcag tggctaatac tgccgtatac ctccgcaggc
      961 cacaccggtt ggcgtagctc gccgattgcg ggcacacatt tatagccata ttcccgcagc
     1021 gcataaattg cagcgatccg cccggctaaa tgttcggctt tgcgcttccg tccagcgtgt
     1081 tgcagttgcg cgtaatgcgg cagccagagt aaatcctgct cacaaaaatt cgccggatcg
     1141 aactcaacaa aatgcagcgt atgtccggca aaggggaggg aggtatgcgt agttttcatg
     1201 tcgaccatat tagagactga tgacaaacgc taaactgcct gatgcgctac gcttatcagg
     1261 cctacatggt cctgcaatat attgaattgg caagattttt gtaggccgga taaggcgttc
     1321 acgccgcatc cggcatgaac gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag
     1381 tgagtgttta cgctcatgta ccacgtacgt cccggctcgt tataggtata cgccccggca
     1441 cccgcgatat agttggcccc tgccagatcg cccgtggtct gggcattacc cgcacgccac
     1501 aaacgtttgt cgaacaggtt gtccacgccg ccggtcagac tgacattctt cgtcacatcc
     1561 caggtcgcgc tcagacccac aatgctgtaa ggactgattt ctttggtttc cgggccaacc
     1621 gctggctgac ctttgtagtt gtattttttc ggctgctgct taccgtacca ggtgaaggtc
     1681 gtttgcatcg acaaatcttc ccgcgcctgc cagctcagcg ttgagttcaa cgtgtactcc
     1741 gggatgatcg acaaacggtc gcccgtggtt ttgttttcac tcttcagcat ataagtgatg
     1801 ttattggtcc acatcaccgt ttcgctaact ggtacgttta acgatccttc cagaccttca
     1861 accactgctt tcggcacgtt atcccactga tagagatcgg tgccgactgc gttttgccct
     1921 acagccacat agcccgcttc aatcttattg cgataatcgt tacggaacca ggtgacgccc
     1981 gccagccagc cgtcgcgttt gaactccaga ccaatctctt tgttgatgct ggtttctgct
     2041 ttcaggtcat cgttaccttg cagatagcag ccgcccgcgc tggcatagca gccctgacct
     2101 ttactgtaga gaatgtagtt cgggttagtc tggtacaggc tcggtgcttt ataagcacgg
     2161 gcaatgccca ttttgagcgt gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg
     2221 ctccagttat tgccgacaat actgtgatga tcgaaacgca gccccggcgt tacgatggtg
     2281 ctgtcagtca gctccatgtt gttttcggca aacagcgaga aaatttctgc ttttgaatac
     2341 gggctacggt cggtggcact cataccatca atagcgccgc cggtgttcgc cccggtcagc
     2401 gcctgggtat tggaactcaa gtccttcatc cgttgctgat tccactccgt acccagcgtc
     2461 agcgtctggt taacgaggat atcaatcggc aggttaactt cgctgtgcag catcacgtca
     2521 tcgagatcga tatcgacgaa atcctgtgtc gctttttcgt taaatttccc ttcggtaccg
     2581 cccgccagac cttccggaat acgcgagtta cgggtgtgtt catactgcac ccagttgctg
     2641 gtggtcacgc cgttatccca gccaccgttc caggtcagcg cgtagttctg gcgatacagg
     2701 cggttggttt catcgccata tttcgagcga gtgtaagcgt cagagttggt gttctgggta
     2761 tcgcccgcat acaggttacc ctggcggctg taacccgctt ccagttccag cgattgcaat
     2821 ggtgcgaagt cccagcgcac cacgccgtta atatctttat tgatcacccc ttcgcgcccg
     2881 gctggtaacg tcgtggcata cgttccggca cgcgcggact gatggccctg gttgatatcc
     2941 cacgcgtcag cctgggtttt gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc
     3001 agcggaccgg tcaggctgaa gttggtgcgt ttagtggctc cttcctcttt atgttctggt
     3061 gcattgaaat aagcgtccca ggagccgtgc cactcaccgc tgcctttttt ggtaatgatg
     3121 ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag ctgccggacc acgcagaact
     3181 tcaatacgtt caatcatttc aggtggcacc caggaggtat caccacgggt atcgcgctcg
     3241 ccacgccagc cctgacgcac cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc
     3301 agcgtgtttt ccggacccat accgcgaata tcaatctgtc ggttattccc acgctgacca
     3361 ctggtggagt taccggtcag gttaacgcct ggcatggtac ggatgatctc cgacacatcg
     3421 cgggcaacgg ggtttttgcg gatttcatct gcggtgatgg tcgaaacgcc aggcgcctgt
     3481 aagttctgct cggcggcggt aacgacaatg gtatcgtcat gtgaaacagg agtatcggtc
     3541 ggctcttgtg cctgcgctac cccataaatc cccagattga ccaacaaggc cagggaatga
     3601 atcttcttgt tcattgtttt attcctgcat ttttgccacg aattgcaact gtcgggcatg
     3661 gtcgtcctca acacgacgca tcccgctacc gcgaaaacct ttgatcctga aagacacgca
     3721 gtgcagttgg ttaataaatg tccgcgcttc ccacggcgcg ccattacgct attgcaaatg
     3781 caaatagtta tcaataatat tatcaatata tttctgcaat caatgaaaaa ttgcacagta
     3841 aacatggggt tatggtgtga cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa
     3901 acatggcccg gaatggcagc gtctgaatga cgaaatgttt gaggtcactt tctggtggcg
     3961 tgatccccaa ggttctgaag aatactcgac gataaagcgc gtatggatct acatcactgg
     4021 tgtgaccgat catcatcaga acagccagcc ccagtcgatg cagcgaattg caggtactga
     4081 tgtctggcag tggacgacac aactcaatgc caactggcgc ggcagctact gctttattcc
     4141 caccgaacgc gatgacattt tttctgcacc atcccccgat cgcctcgaat tacgtgaagg
     4201 ctggcgaaaa ctattacccc aggcaatagc cgatccgctg aatccacaaa gctggaaagg
     4261 cgggcgaggg cacgctgttt ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg
     4321 ggattgtccg caagcgccag aaatagcagc caaagaaatt atctggaaaa gtgaacggtt
     4381 gaaaaattca cgtcgtgtat ggatttttac caccggcgat gcaacagcag aagaacgtcc
     4441 gctggcagtt ttgctcgatg gcgaattttg ggcgcaaagt atgcccgtct ggcctgtgct
     4501 gacttcgctg acccatcgtc agcaacttcc tcccgccgtg tatgtgttga tcgacgctat
     4561 cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg gatttctggc tggcagtaca
     4621 gcaagagtta ttacccctgg tgaaagctat tgcccctttt agcgatcgcg ccgatcgcac
     4681 cgtggtcgcc gggcagagtt ttggtgggct gtccgcgctg tatgccggac tgcactggcc
     4741 tgaacgcttt ggctgtgtat taagccagtc aggatcgtac tggtggccgc atcggggcgg
     4801 gcagcaagag ggcgtgttac ttgaaaagct taaagctggt gaagttagcg ccgaaggtct
     4861 gcgcattgtg ctggaagcgg gtattcgcga gccgatgatt atgcgggcca atcaggcgct
     4921 gtatgcgcaa ttacacccca taaaagaatc cattttctgg cgtcaggttg acggcggaca
     4981 tgatgcgctt tgttggcgcg gtggcttgat gcaggggcta atcgacctct ggcaaccact
     5041 ttccatgaca ggagttgaat atggcattca gtaatccctt cgatgatcca cagggagcgt
     5101 tttacatttt gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa tgcgccttac
     5161 cggcaggctg ggacattgtg tgtcagccgc agtcacaggc gtcctgccag cagtggctgg
     5221 aagtccactg gcgtactctg acaccggcca atttcaccca gttgcaggag gcacaatgag
     5281 ccagcattta cctttggtcg ccgcacagcc cggcatctgg atggcagaaa aactgtcgga
     5341 tttaccctcc gcctggagcg tggcgcatta cgttgagtta actggagatg ttgatgcgcc
     5401 attactggcc cgcgcggtgg ttgtcggact agcgcaagca gatacgttgc ggatgcgttt
     5461 tacggaagat aacggcgaag tctggcagtg ggtcgatgat gcgctgatat tcgaactgcc
     5521 agaaattatc gacctgcgaa ccaatattga tccgcacggt actgcgcagg cattaatgct
     5581 ggcggatttg caacaagatt tgcgcgtcga tagcggtaaa ccactggtct ttcatcagct
     5641 gatacatgtt gcggataacc gctggtactg gtatcagcgt tatcaccatt tgctggtcga
     5701 tggcttcagt ttcccggcca ttactcgcca gatcgccaat atttactgca catggctgcg
     5761 tggcgaacca acgcctgctt cgccgtttac gcctttcgct gatgtagtgg aagagtacca
     5821 gcaataccgc gaaagcgaag cctggcagcg tgatgcggca ttctgggcag aacagcgtcg
     5881 tcaactgccg ccgcccgcgt cactttctcc ggcaccttta gcggggcgca gcgcttcggc
     5941 agatattctg cgcctgaaac tggaatttac cgacggggaa ttccgccagc tggctacgca
     6001 actttcaggt gtgcagcgta ccgatttagc ccttgcgctg gcagccttgt ggctggggcg
     6061 attgtgcaat cgcatggact atgccgccgg atttatcttt atgcgtcgac tgggctcggc
     6121 ggcgctgacg gctagcggac ccgtgctcaa cgttttaccg ttgggtattc acattgcggc
     6181 gcaagaaacg ctgccggaac tggcaacccg actggcagca caactgaaaa aaatgcgtcg
     6241 tcatcaacgt tacgatgccg aacaaattgt ccgtgacagc gggcgagcgg caggagagga
     6301 accgctgttc ggtccggtac tcaatatcaa ggtatttgat taccaactgg atattcctgg
     6361 tgttcagacg caaacccata ccctggcaac cggtccggtt aatgaccttg aactggccct
     6421 gttcccggat gaacacggtg atttgagtat tgagatcctc gccaataaac agcgttacga
     6481 tgagccaacg ttaatccagc atgctgaacg cctgaaaatg ctgattgccc agttcgccgc
     6541 ggatccggcg ctgttgtgcg gcgatgtcga tattatgctg ccaggtgagt atgcgcagct
     6601 ggcgcagatc aacgccactc aggttgagat tccagaaacc acgctgagcg cgctggtggc
     6661 agaacaagcg gcaaaaacac cggatgctcc ggcgctggca gatgcgcgtt acctgttcag
     6721 ctatcgggaa atgcgcgagc aggtggtggc gctggcgaat ctgctgcgtg agcgcggcgt
     6781 taaaccaggg gacagcgtgg cggtggcact accgcgctcg gtctttttga ccctggcact
     6841 ccatgcaata gttgaagctg gcgcggcctg gctaccgctg gataccggct atccggacga
     6901 tcgcctgaaa atgatgctgg aagatgcgcg tccgtcgctg ttaattacca ccgacgatca
     6961 actgccgcgc tttagcgatg ttcccaattt aacaagcctt tgctataacg ccccgcttac
     7021 cccccagggc agtgcgccgc tgcaactttc acaaccgcac cacacggctt atatcatctt
     7081 tacctctggc tccaccggca ggccgaaagg ggtaatggtc gggcagacgg ctatcgttaa
     7141 ccgcctgtta tggatgcaaa atcactaccc gcttacaggc gaagatgtcg ttgcccaaaa
     7201 aacgccgtgc agttttgatg tctcggtgtg ggagtttttc tggccgttta tcgcaggggc
     7261 aaaactggtg atggctgaac cggaagcgca ccgtgacccg ctcgctatgc agcaattctt
     7321 tgccgaatat ggcgtaacga ccacgcactt tgtgccgtcg atgctggcgg catttgttgc
     7381 atcgctgacg ccgcaaaccg ctcgccagaa ttgcgcgacg ttgaaacagg ttttctgtag
     7441 tggtgaggcc ttaccggctg atttatgccg cgaatggcaa cagttaaccg gcgcgccgtt
     7501 gcataacctg tatggcccga cggaagcggc ggtagatgtc agctggtatc cggcttttgg
     7561 cgaggaactg gcacaggtgc gcggcagcag tgtgccgatt ggttatccgg tatggaatac
     7621 gggtctgcgt attcttgatg cgatgatgca tccggtgccg ccgggtgtgg cgggtgatct
     7681 ctatctcacc ggcatccaac tggcgcaggg gtatcttgga cgacccgatc tgaccgccag
     7741 ccgttttatt gccgatcctt ttgccccagg tgaacggatg tatcgtaccg gagacgtcgc
     7801 ccgctggctg gataacggcg cggtggagta cctcgggcgc agtgacgatc agctaaaaat
     7861 tcgcgggcag cgtatcgaac tgggcgaaat cgatcgcgtg atgcaggcgc tgccggatgt
     7921 cgaacaggca gttacccacg cctgtgtgat taaccaggcg gcagccaccg gtggtgatgc
     7981 gcgtcagttg gtgggctatc tggtgtcgca atcgggactg ccgttggata ccagcgcatt
     8041 acaggcacag cttcgcgaaa cattgccgcc gcatatggtg ccggtcgttc tgctgcaact
     8101 tccgcagttg ccgcttagcg ccaacggcaa gctggatcgc aaagccttac cgttgcctga
     8161 actgaagaca caagcgtcgg gacgtgcacc gaaagcgggc agtgaaacga ttatcgccgc
     8221 ggcattcgcg tcgttgctgg gttgtgacgt gcaggatgcc gatgctgatt tctttgcgct
     8281 gggcggtcat tcgctactgg caatgaaact agcagcgcag ttaagtcgac agtttgctcg
     8341 tcaggtgacg ccggggcagg tgatggtcgc gtcaaccgtc gccaaactgg caacgattat
     8401 tgatggtgaa gaggacagct cccggcgcat gggattcgaa accattctgc cgttgcgtga
     8461 aggtaatggc ccgacgctgt tttgtttcca tccggcgtcc ggttttgcct ggcagtttag
     8521 cgtgctctcg cgttatctcg atccacaatg gtcgattatt ggcattcagt cgccgcgccc
     8581 tcatggaccc atgcagacag cgacaaacct ggatgaagtc tgcgaagcgc atctggcaac
     8641 gttacttgag caacaaccgc acggccctta ttacctgctg gggtattccc ttggcggtac
     8701 gctggcgcag ggtattgcgg cgcgactgcg tgcccgtggc gaacaggtgg catttcttgg
     8761 tttgctggat acctggccgc cagagacgca aaactggcag gaaaaagagg ctaatggtct
     8821 ggacccggaa gtgctggcgg agattaaccg cgaacgcgag gccttcctgg cggcacagca
     8881 gggaagtact tcaacggagt tgtttaccac cattgaaggc aactacgctg atgctgtgcg
     8941 cttgttaacc accgcccata gcgttccgtt tgatggaaaa gcaacgttat ttgttgctga
     9001 gcgcacactt caggaaggta tgagtcccga acgtgcctgg tcgccgtgga ttgcggagct
     9061 ggatatctat cgtcaggatt gtgcgcatgt ggatattatc tctccagggg cgtttgtaaa
     9121 aattgggccg attatt
//
LOCUS       DAZFRJ010000172         8958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000172 DAZFRJ010000000
VERSION     DAZFRJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.174"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1079
                     /locus_tag="ACHKUN_003768"
     CDS             <1..1079
                     /locus_tag="ACHKUN_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6661951.1"
                     /translation="YLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLA
                     LFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTA
                     TWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYT
                     IWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLS
                     RPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVF
                     GLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLV
                     MTEADKALREAKSEGGNKVIIHHI"
     gene            1260..2750
                     /gene="dgcJ"
                     /locus_tag="ACHKUN_003769"
     CDS             1260..2750
                     /gene="dgcJ"
                     /locus_tag="ACHKUN_003769"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO6661952.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2793..3296
                     /gene="yeaK"
                     /locus_tag="ACHKUN_003770"
     CDS             2793..3296
                     /gene="yeaK"
                     /locus_tag="ACHKUN_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO6661953.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3297..3401)
                     /gene="yoaI"
                     /locus_tag="ACHKUN_003771"
     CDS             complement(3297..3401)
                     /gene="yoaI"
                     /locus_tag="ACHKUN_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO6661954.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3571..4017
                     /gene="yeaL"
                     /locus_tag="ACHKUN_003772"
     CDS             3571..4017
                     /gene="yeaL"
                     /locus_tag="ACHKUN_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO6661955.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3974..4795)
                     /gene="nimR"
                     /locus_tag="ACHKUN_003773"
     CDS             complement(3974..4795)
                     /gene="nimR"
                     /locus_tag="ACHKUN_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO6661956.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4892..6073
                     /gene="nimT"
                     /locus_tag="ACHKUN_003774"
     CDS             4892..6073
                     /gene="nimT"
                     /locus_tag="ACHKUN_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO6661957.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRTLHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6128..6475
                     /gene="yeaO"
                     /locus_tag="ACHKUN_003775"
     CDS             6128..6475
                     /gene="yeaO"
                     /locus_tag="ACHKUN_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO6661958.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6497..6751)
                     /gene="yoaF"
                     /locus_tag="ACHKUN_003776"
     CDS             complement(6497..6751)
                     /gene="yoaF"
                     /locus_tag="ACHKUN_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO6661959.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6934..7959
                     /gene="dgcP"
                     /locus_tag="ACHKUN_003777"
     CDS             6934..7959
                     /gene="dgcP"
                     /locus_tag="ACHKUN_003777"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO6661960.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            7992..8090
                     /gene="yoaK"
                     /locus_tag="ACHKUN_003778"
     CDS             7992..8090
                     /gene="yoaK"
                     /locus_tag="ACHKUN_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO6661961.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8093..8167
                     /gene="yoaJ"
                     /locus_tag="ACHKUN_003779"
     CDS             8093..8167
                     /gene="yoaJ"
                     /locus_tag="ACHKUN_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO6661962.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8226..8474)
                     /gene="yeaQ"
                     /locus_tag="ACHKUN_003780"
     CDS             complement(8226..8474)
                     /gene="yeaQ"
                     /locus_tag="ACHKUN_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6661963.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8702..>8958)
                     /locus_tag="ACHKUN_003781"
     CDS             complement(8702..>8958)
                     /locus_tag="ACHKUN_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6661964.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2215 a         2168 c         2110 g         2465 t
ORIGIN      
        1 tctatttagt tgaaaccatt gtaattatcc ataaaccaat taacggcagt acactgatcc
       61 agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt tttatttgtt
      121 taacctcgct ggcactcttt tgttatggaa aagacaacat ccttgacaac aataagaaaa
      181 aaacgggaat cctgttgctg gcgctgattc cttttttagt ttttcccctt ctggcacaca
      241 atctgagcag ttataacgct gactattctt tgtatgtcgt cgactactgt ccggacaacc
      301 atactgcgac ctggggaatc aactatacaa aaatattggt ttgtctgtgg gcatttttac
      361 tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta atagcattat
      421 tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag tataattaca
      481 ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt gtgtcttttc
      541 tgatttataa catttttcag gaactgcaac tctccagcaa actggcagtt catgatgtgc
      601 tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta ttgtcgcgac
      661 ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa cgcatcaatg
      721 cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat attatccagc
      781 aaagcatccg ccctgatgat attttagcgc gactggaggg tgaggtgttt ggcttgctat
      841 ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt aaaaatgtcg
      901 aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc atcagtattg
      961 gcacggtttt ttcaacgggt gacacgcgta atatctcgct tgtcatgacg gaagcagata
     1021 aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat catatttaag
     1081 tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt attactatta
     1141 cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc cctgtgccag
     1201 gccgcaatcg aactttatct ggttttctcg tttcactaac cgaaggagtg ccatttatca
     1261 tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt gtgctgacat
     1321 cttccttcct tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc tatctgcgct
     1381 atatcgtgca gaaagcagac tcctcctttc tttatgataa gtatcagaat cagagtattg
     1441 ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca gaacagcggc
     1501 gcgccatctg cgaggctttt gagtctgcca ataataccca tggcttaaac ctgactgccc
     1561 ataaataccc gggcttacgc ggcacactac aaaccgcatc cactgactgc gacacaattg
     1621 tggaagctgc agcactatta cccgcttttg atcaggcagt ggaaggcaac cgccaccagg
     1681 atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat ctcgatctca
     1741 atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg atgaataact
     1801 ggtccttcct gcagtcagga agtattggca tcgatcgcaa agatattgaa aaggtattta
     1861 ccgggcgtac cgtattgtcg agcatttacc aggatcagcg tactaaacag aacgtgatga
     1921 gtttgctgac gccggtatat gtcgcagggc agctaaaagg gattgtgctg ctggatatta
     1981 acaaaaacaa tttgcggaat atcttttaca ctcatgaccg ccctctcctc tggcgttttc
     2041 tcaatgtcac gctaaccgat accgattcgg ggcgcgacat tatcatcaac cagagcgaag
     2101 ataatctgtt ccagtacgtc agttacgtcc atgacttacc gggcggcatt cgtgtctcgt
     2161 tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg ttctggattt
     2221 tgacggcgtt aattttgctg aatatggtcc ggatgcactt ccgtttatac caaaatgtgt
     2281 cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt ttaacccctg
     2341 aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg tttattgcaa
     2401 ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag ggggatttag
     2461 cgattacgtt attagctcag gcgattaaac aatcgattcg taaaagtgat tatgccatcc
     2521 gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa attgcagcac
     2581 aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa gaaatcggct
     2641 tctcttccgg tatttacgcg atgaaagaaa acgatacgtt acatgatgcg tataaagctt
     2701 ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga taaccttctg
     2761 tggttgtttg cttgtaatct caggagcgtg aaatgactga aatggctaaa ggaagcgtga
     2821 cgcatcagcg gttaatcgct ttattatcac aagaaggtgc taacttccgc gtcgttaccc
     2881 atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct ctaggccagg
     2941 gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat gttctggcga
     3001 tcctcgctgc cgatcaacag gccgatctga gccaactcgc cagccatata ggcggattac
     3061 gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac aggctgtgtc ttcggcgcga
     3121 tccccccttt cagcttccat ccaaaactca aactggttgc cgaccctcta ctcttcgaac
     3181 gatttgatga aatcgccttc aatgcaggga tgctggataa atccgttatt ttgaaaacca
     3241 cggattattt gcgcattgcg caaccagaac tgctcaattt ccgccgcact gcgtaactag
     3301 cctgtccgtt caataagcag aacggacaaa accagcacaa cagcaatggc aaaaaacgat
     3361 gacgtgataa tcagtgtctc gacgaacatt tgatcgttca tagcatgccc ccgggagata
     3421 gctgttttca cgttattgtt agcgtgcaca aatggcagtt tgatgacagt tcgcctgatt
     3481 ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt aaagtaagca
     3541 ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct gatcctgctc
     3601 gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc aattctggtg
     3661 ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga aaaacagggg
     3721 ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcgc cagcgggacg
     3781 ctaccgccct caaccttgat tcactcgttt ctgaactgga aatcgctggt ggcaattgca
     3841 gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga ccttaatggg cagccagccg
     3901 caactggtcg ccgggctgct ggtaggcact gttttaggcg tggcgctgtt tcgcggcgta
     3961 ccggtcggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa acagtagtta
     4021 atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata gtaataaagg
     4081 ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg cctttcacta
     4141 atccctgaag tgccataatc agctgtaact gctgacgcca ctggcgaaaa ctcaaccccg
     4201 tctcttttac tatcagtcgc gcaagattgc gttcactcat cgcaaaaaag ccagcccatt
     4261 gccctaatga cccccactcg acgggccctt tcgccatcat ctccaccatc gtgcggattt
     4321 taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg agttcatcaa
     4381 acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc gtcctgtttg
     4441 ccagggttaa aatcaattcc cggcataagg gcgatatttt caatgtgcaa catgttgtcg
     4501 gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta gctgtcactt
     4561 gattgctatg ttccaccccg cccggtatcc aaaccgcata ttgcggcggc accatccaca
     4621 aagcattttc caccgtacag gtaattgcac catgtaatgc tagtatcagc tgtcctttgc
     4681 ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa aatgcgaccg
     4741 cggcttcatg gtgtcggtcg ggttcatagc cattgagatt caacctgtgc atcattttgt
     4801 ccgaatttag cgataatttg tcattttagc ttgattcaac ataacaataa aaacggtaag
     4861 gtacagcctc gtttgtaaca atgagaagca tatgacctgt tcaacttcat taagcggcaa
     4921 aaacaggatt gtccttatcg ctggcattct gatgattgcc actacattac gcgtcacctt
     4981 caccggcgca gcaccgttac tcgatacgat tcgttccgct tactcgctga cgacagcgca
     5041 aaccggctta ttaaccaccc tgccattatt ggcctttgcg ctaatctcac ctttggctgc
     5101 cccggtggcg cgacgttttg gtatggaacg tagcctgttt gccgcgttac ttttgatctg
     5161 tgcaggtatc gcaattcgtt ctctcccttc gccttactta ttatttggcg gtacagcggt
     5221 cattggcggt gggattgcat taggcaatgt cttactgcca ggattaatta aacgcgattt
     5281 ccctcattcc gtcgccagac ttaccggcgc atattccctg acaatgggag ctgcagcggc
     5341 actgggatcg gctatggtcg tgccgctggc tttgaacggt tttggctggc aaggcgcgtt
     5401 gttcatgctg atgtgttttc ctctgctggc tcttttttta tggctgccac agtggcgaag
     5461 ccaacaacat gcaaatttga gtacctcgcg caccttacat actcggggta tctggcgttc
     5521 gccgcttgcc tggcaggtca cattgtttct tgggatcaac tcactggtct attacgtgat
     5581 tattggctgg cttccggcga tcctcatcag tcgcggctat agcgaagcac aggcgggttc
     5641 actgcatggt ttgctgcaac tagccacagc agctcccggt ttgctgatcc ctcttttctt
     5701 acatcatgtg aaagatcagc gtggtattgc agcgttcgtt gccttgatgt gcgcagtggg
     5761 cgctgttggg ctctgcttta tgccagcgca cgcgatcacc tggactctgc ttttcggttt
     5821 tggttccggc gcaacaatga tactggggtt gacgttcatt ggtcttcggg caagttctgc
     5881 gcatcaggcg gcggcactct cggggatggc acaatccgtc gggtatttgc tggcagcctg
     5941 tgggccgccg ctgatgggta aaatacacga tgctaacggt gactggtccg taccactgat
     6001 gggtgttgcc atactttcac tactgatggc gattttcgga ctttgcgccg ggagagacaa
     6061 agaaattcgc taatatccgg tgctatagtg acgtgacaaa tcatgcgtga aagggagaac
     6121 aaacacgatg aatattcagt gcaaacgcgt ttatgatccg gctgaacaga gcgatggtta
     6181 tcgcgtactg gtcgaccgcc tctggccgcg cggtatcaaa aaaaccgatt tagcccttga
     6241 tgagtgggat aaagaaatca cgccgtcaac ggaactgcgc aaagcctttc acggcgaagt
     6301 cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac acgagcaaga
     6361 aggaaagcgg ctggcggaca tcgccaaaaa acagccgctg accctgctct acgcagcaaa
     6421 aaacaccacg cagaaccatg cgctggtgct ggccgactgg ctacgtagct tgtgatttta
     6481 gtacagcatc cggcggttat ttttcaccag ccggatggtc ccgtcgccac aatgcccatt
     6541 catcaatcgt ttcaccgccc ggtaatttgc aattgttgct gaccccttgc gctgtctgca
     6601 ctggaatgag cgtcccgccc ttctgctggc aatagaccga cgccggattt gccataccaa
     6661 tctgcggcgg tttaggtgct tctggctgag aagaggttga acaaccagcc aggaccagca
     6721 agcaaggcaa agcaaaactg ataattttca tttattgatc tcacacattt atctacgctt
     6781 agagtatcgc ggtatcgttt tgttttgcag cactattttt attacattca ctcaaaacat
     6841 attacgtctt gtttcatctt tgttgatgat gttttatcat gcctgcaaag attaaataat
     6901 cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag atcagattat cgcccgcgtc
     6961 tcgcaatccc ttgccaaaga acagtcacta gaaagtctgg tccgacagct tctggagatg
     7021 ctggaaatgg tcactgatat ggaatcaacc tacctgacca aagtggatgt cgaagcgcgc
     7081 ctgcagcata ttatgtttgc ccgtaacagc cagaaaatgc acatcccgga gaattttacc
     7141 gtctcgtggg attactcgtt atgcaaacgc gctattgatg aaaactgctt tttcagcgat
     7201 gaagtccctg accgttgggg cgactgtatt gcggcacgca atcttggaat caccacattt
     7261 ctgagtacac caattcactt accggatgga tcattctatg gcacgctttg cgccgccagc
     7321 agtgagaagc gccagtggag tgaacgcgcg gaacaggttt tacagttatt cgccggactg
     7381 attgcacaat atattcaaaa agaggcgttg gttgaacagc tgcgcgaagc caatgccgcg
     7441 ctgattgcgc aatcgtatac cgactcgtta accgggctac cgaatcggcg ggcgattttt
     7501 gaaaatctga cgacgctgtt ttctctcgcc cggcatctta accataagat aatgatcgcg
     7561 tttatcgatc tggataactt caaattaatc aatgatcgtt ttggtcataa tagtggcgat
     7621 ctgtttctca ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg cgaagttatt
     7681 ggtcgtctcg gcggtgatga gtttttggtt gtttcactaa ataacgagaa tgcggatatt
     7741 tcgtcgctgc gagaacgtat tcaacagcaa atacgtggag aatatcactt aggtgatgtt
     7801 gatttgtatt atcctggtgc cagtcttggc attgtagaag tcgatcctga aacgaccgat
     7861 gcagacagtg ccctgcatgc tgccgatatc gcgatgtatc aggagaaaaa acacaaacag
     7921 aaaacacctt ttgtcgcgca ttcagcgcca cattcctgag gcgtattcac atccttttga
     7981 ttggtgataa catgcgaatc ggtattattt ttccggttgt aatctttatt acagcggtcg
     8041 tatttttagc atggtttttt attggcggct atgctgcccc gggagcataa agatgaaaaa
     8101 aacaacgatt attatgatgg gtgtggcgat tattgtcgta ctcggcactg agctgggatg
     8161 gtggtaacgt cacctctaaa aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt
     8221 aagcgttaac ttttaatctt cctgtagata aatagcacga caatcgcacc aataacggca
     8281 accacgaagc tgccaaaatt gaagccatcg actttaccaa agccaaacag cgtgctgatc
     8341 catccgccaa ctacggcacc gactatcccc agcaggatag tcataaagaa tccaccacca
     8401 tctttacctg gcatgatcca cttcgccaga ataccggcaa taagcccaaa aataatccat
     8461 gacagaatgc ccattgtttc ctcacttatc tgttttgcat tagcgggtta gtcgctgata
     8521 aaaagcatag cacaacatcg ggagggcaag atttgtgacg agcatcacgg aggatttttt
     8581 tgcggatggc acagaaattg cgccatcaac gatcagtgat aattaccaac cacaaacatc
     8641 atgttcgttt tccgtgtctt gacatcctcc acgccctgaa tgacgaggat ccctgctacg
     8701 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgggcgg cctgactgca
     8761 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccgttta
     8821 catcggcgtt cgctgtatat ccacatacct ggcatctgaa ttgactttgt gacaggcgat
     8881 tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag
     8941 caagcacctg accgccac
//
LOCUS       DAZFRJ010000173         8863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000173 DAZFRJ010000000
VERSION     DAZFRJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.175"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..2339
                     /gene="katP"
                     /locus_tag="ACHKUN_003782"
     CDS             129..2339
                     /gene="katP"
                     /locus_tag="ACHKUN_003782"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO6661965.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2383..2772
                     /locus_tag="ACHKUN_003783"
     CDS             2383..2772
                     /locus_tag="ACHKUN_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6661966.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            2886..3653
                     /locus_tag="ACHKUN_003784"
                     /pseudo
     CDS             2886..3653
                     /locus_tag="ACHKUN_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3166..3276
                     /locus_tag="ACHKUN_003784"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(3733..4128)
                     /locus_tag="ACHKUN_003785"
     CDS             complement(3733..4128)
                     /locus_tag="ACHKUN_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6661967.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(4128..5087)
                     /locus_tag="ACHKUN_003786"
     CDS             complement(4128..5087)
                     /locus_tag="ACHKUN_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO6661968.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5360..6262
                     /locus_tag="ACHKUN_003787"
     CDS             5360..6262
                     /locus_tag="ACHKUN_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6661969.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            6259..6570
                     /locus_tag="ACHKUN_003788"
     CDS             6259..6570
                     /locus_tag="ACHKUN_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661970.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            6646..7329
                     /locus_tag="ACHKUN_003789"
     CDS             6646..7329
                     /locus_tag="ACHKUN_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6661971.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            7329..7547
                     /locus_tag="ACHKUN_003790"
     CDS             7329..7547
                     /locus_tag="ACHKUN_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661972.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            7507..7993
                     /locus_tag="ACHKUN_003791"
                     /pseudo
     CDS             7507..7993
                     /locus_tag="ACHKUN_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8038..8277
                     /locus_tag="ACHKUN_003792"
                     /pseudo
     CDS             8038..8277
                     /locus_tag="ACHKUN_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKUN_003793"
                     /pseudo
     CDS             8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKUN_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            8517..>8863
                     /locus_tag="ACHKUN_003794"
     CDS             8517..>8863
                     /locus_tag="ACHKUN_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6661973.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGER"
BASE COUNT         2178 a         2044 c         2330 g         2311 t
ORIGIN      
        1 tgcggcttgg aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat
       61 cctgttgcat caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag
      121 ctattcgtat gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct
      181 tttctaccgc tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac
      241 tggatttaac tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg
      301 attatgccac cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt
      361 tgctgacaac ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta
      421 ttcgtatggc ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca
      481 gtggtggtca gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata
      541 aagcccgtcg attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag
      601 acctgatggt cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat
      661 ttgctggcgg aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca
      721 agcctcttgc agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc
      781 agatgggact tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt
      841 ccgcgaaaga tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg
      901 ccctgatcgc gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat
      961 gtattggcgc agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata
     1021 aatgtggtac aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga
     1081 catcgccaac ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc
     1141 tgcacaagag tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc
     1201 aggacgcgca tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc
     1261 ttaaagttga tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt
     1321 ttgagcaggc attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag
     1381 cccgatatct tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg
     1441 cggcggatta tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg
     1501 atttgggtat ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc
     1561 gtgtgactga ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta
     1621 actgggaagt taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac
     1681 agcgtgaatt taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg
     1741 ttctttcggg taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga
     1801 ttccctttac tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca
     1861 gtgtactgga gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata
     1921 tatcgccggt tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa
     1981 tgacggcatt actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg
     2041 gagtgtttac cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata
     2101 tgtcaacacg atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa
     2161 cgggagcatt aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
     2221 tacgtgcggt ggcagaagta tatgcctcgg atgatgcgag aaataagttt attcatgatt
     2281 ttgttaaatc gtggaataaa gttatgaata gcgatcggtt tgatttaaac aataaatgat
     2341 aaaactgatg agggagggca cctccctcat gaaggagaaa ttatgcagaa aagcaataaa
     2401 gttataatca gctgtggtat ggcattatat tttttgtgtg gggggattac agtggcagaa
     2461 gaacttgatg aaagcatgca agttcttgca aggaatatga gtgtacttca gtcatcaatg
     2521 gataaaaagg agcttcttga agctcttgac agaatggatg atgctgtaga tgagtcaatg
     2581 aagcgtcttc ctgagagcat ttcaccggct gatgaagaag ggaagaatga ctatataaaa
     2641 gaacttcaaa agcttgaaaa agagattatg ctggcgaaac aaaaagtctt atcgggacag
     2701 ttatcagata tacccgattc ggtaaacaag atgaataaaa tcagagttga ggggcatgat
     2761 aaattcagat aaatcagttg gttgattcct gcgggacgaa tacatacgtc aggaacataa
     2821 ctttatggca gtgaaccgcc ccgggaatcc tggagactaa acttcctgag aaagaggtaa
     2881 acaggatgac taaaaatact cgtttttccc ccgaagtccg tcaacgggcg attcatatgg
     2941 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttgttcc attgcccaaa
     3001 gattggctgt acaccggaga ctctgcgtgt ctggggacgc cagcatgagc gggataccgg
     3061 gagcggtgat ggtgggctca ccagcgctga acgtcagcgt ctgaaagagc tggaacgtga
     3121 aaaccgtgaa ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa
     3181 ggccgagttc gaccgcctct ggaaaaaata atgccactgc tgctgcgtga gcagtacggg
     3241 gtcggaccgg tatgcagtga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa
     3301 cagcggtatc atccttataa acgtagtgcc tgtgcacagc gcgatgactg gctgaagaaa
     3361 gagatactgc gcttatacga tgagaatcat caggtatacg gtggcgtaaa gtctgacgtc
     3421 agttgttaca ggaaggtatc agggtggcca gatgtatagt ggcgcgcctc atggcggtta
     3481 tgggacttgc cggtgttctc cggggtaaaa aggtccgtac taccgtcagc cggaaagccg
     3541 tttccgcagg cgaccgcgta aaccgtcagt tcgtggcaga acgtcctgac cagctgtggg
     3601 tggctgattt tacttacgtc agcacatggc agggcttcgt ctgtgtggcg ttcgatttag
     3661 ggcactaaaa ccgctgctgg tccctcataa taaaagaaga gcaggtaata gacttaacct
     3721 gctcttcttt tattattttt ttaacataga aagattgcgt ctggtttgtt cttcgaaatc
     3781 atcttttgaa gtgatgctct ctgatttgtt ttctgaagat gcccctatga tgttttttaa
     3841 cacacttata tcaattgatg tagatgtgta tcctgttgtt tgctctataa gtttcaccag
     3901 attttctgca gtgattttct cactgaaaaa accactgatt agcgttaaca gtcgggggtc
     3961 aacagaataa agagctgctc cgcaaataag agattgtctg tagaagtctc cccgcatttg
     4021 tatcgggaga gagtctattg tttgttgtgt caggttatcc tgcggataaa gtgtcgggtg
     4081 caggtatgcc acaactttct ttcttttttt cggatcactg ctttccatta cgcatgcacc
     4141 attgctttca ccaatgcaaa ctgaggttca tccaccttgt tcactttcag attcgggaaa
     4201 tattgcttta tgtgagggta aataagctcg gctcctccgc cagtgagata gattcggttc
     4261 attcctgaga atgttgaaat ttcctgcctg atgctttctg ccagagattt cacttccctg
     4321 tctattacat gagtaatgtt ttttatttca gctacatcat ttattaatgt ctggagatat
     4381 tcattatcat gtctgttttt tattaactca tcagcaataa aataattaca tggtgtggag
     4441 gctttcgtca gcgcattcat gacggtatga gtgatgcggg atgtcccgat ttctgaatac
     4501 ccacggattt cagaaatgcc ttcaaatgca ccgagaatta aaccacaatc cagagtggtt
     4561 cctccaagat cgataatcag gctgcgttca agtttgttta ctttatcttt ttttagtgat
     4621 tcaaatgcag ctggcaaaga ttcaggcatt acattaactt ttttaatttt aaatgtttcc
     4681 cctttattca gagatatctc acggagaaca tttgcctttt ttctttctat tctttcttca
     4741 tttggttgat tgtcattatc aaagaattcg gttacaggta atgtcactgt cagttctaca
     4801 tcctgtggct gaaggccgga tgtcagcagt gcatgatgta tggcaagcaa attggtggtg
     4861 ctgtattgat aactgacatg tgttgtacct atgacagcag tactgcccaa atcataggtg
     4921 tatttttttc catccacgac gtaattgtaa actttgccgg cattaaacag tcccgggttc
     4981 cagccttctt taaagctgtt tcctgacaga ctggttctta cctcgccatc ttcaagccag
     5041 gccagtttta cgttagttga accatcatcg catgatactt ttagcatttt ttcttgcctc
     5101 gttttgagtt tttatttatc tcaaattgag catcctttat ctagatgcat ctttttgagt
     5161 tttttgttgt tccttattgt gtctcatttt gggttcatgt caagcgatga ggctctttta
     5221 gagtctcttt gcattcttgt tgtgactcag tttgagtctc tatcgatttc tcattttgcg
     5281 tttcgaactg agacggaaac tacttaacca ggccgcatgt gagaggagaa ttcccatgcc
     5341 aaactggtgc tcaaatcgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa
     5401 acgactggcc agcggtgccg tcacaccgct ttatcgccgc gccacaaatg aaggtattca
     5461 gctgtttctg gccggaagtg ccggacttct gcagaccact gaagatgtgc ggtttgaacc
     5521 atgccccgga ctgacggctg ccgggcgtgg cgttgtatcg ccggagaata tcgcgttcac
     5581 ccgctggctg acatacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct
     5641 gcatgaactc tggctgcaga gcggtactgg acagcgtcgc tgggaaggat taccggacga
     5701 tgtcagggat accatcaccg cacttttcac cgcaaaaaga ggtgactggt gtggcttctg
     5761 gagtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtac tgccggaaaa
     5821 aaccatgccg tttgacctgc tgacggttct gccgacccgc ctggatgttg aagtgaatgg
     5881 ctttaacggt ggtgttctga acggtgttcc ttctgcatat cactggtata cggaacagta
     5941 tggcgtgaag tggcctgtgg ggtatgaggt gaatatcagt agtcagggag acaacttcat
     6001 tcaggttgat ttcgacacgc cgtggtatca gccggaaagc gacgttattg cagaattaag
     6061 ccgccgtttc agctgcacac tggagcactg gtatgccgaa cagggctgca atttctgtgg
     6121 ctggcagcga tatgagcgtg gcgagcttgt tgatgtgctg tggggggagc tggaatggtc
     6181 acatccgaca gatgatgacg agttgccgga agtcaccgga cctgcgtgga tagtcgacaa
     6241 ggtggcgcat tatggcggat gaagtatgac ggagacgggc gggcaactgc ccgtttcttt
     6301 tccggtaaag gatgtcgccg tgctcctctt tttactgcgc cggctgacgc ggcgcggcat
     6361 aaacgctgtc tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc
     6421 tcccggctgg cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct
     6481 tcaagccgct ttcgcggcat agccttgccg tcaaaaagac gaaggctgcg gtattccacg
     6541 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggt aacgggcacc
     6601 acaggggtgc cctcccggtg ccctctgtaa gagaaggagt tttgtatgtc ccgttttgtc
     6661 ctcggtaact gcatcgatgt tatggcccgt attcctgata acgccattga tttcatactg
     6721 actgacccgc cgtacctcgt cggtttccgt gaccgttccg ggcgcaccat tgccggtgac
     6781 gttaatgatg actggctgca acctgccagc aatgagatgt accgcgtact caaaaaagac
     6841 gcgctgatgg tcagcttcta cggctggcac cgtattgacc gctttatggc ggtctggata
     6901 cgcgccggat tcagcgtggc agggcacctc gtgttcacca aaaactacac atccaaatct
     6961 gcgtatgtcg cgtaccgcca cgaatgcgct tacatcctgg caaagggcag tccggcccgg
     7021 ccccgtaacc cgctgccgga tgtgctgggc tggaaatatt cgggcaaccg ccatcacccg
     7081 acggaaaagc ccgttaccag cctgcaaccg ctgattgaga gcttcacaca cccgaactca
     7141 attgtgctgg acccgtttgc aggcagcggt tcaacctgcg tggccgccct tcagtccgga
     7201 cgccggtata ttggtatcga gctgcttgaa cagtatcacc gtgccggaca gcaacgcctt
     7261 gccgccgtgc aacgggccat gcagcagggg gccgcaaatg ataacaggtt tgaaccggag
     7321 gcagcatgat gaattacagc ggatttgaag atatgcgtga acaggtggct gaactggcca
     7381 acaccatgtg cgacctgcgc acaaccatga atgagatgga gcagcggtac agcgatgcag
     7441 acaccctgcc ggaacgtctg gtgcgtcaga cgctctttcg tgccagccac ctgtttatga
     7501 aggcatataa cgaaatactg gaactggatt actgcttcaa agattgaggg gaaagagtat
     7561 gtacggaaca tgcgaaacgc tctgccgggc gctggcagca aagtatccgg gagacacgcc
     7621 gttgatgctg gttatctggt ccccggaaga gattcaggcc ctcgctgacg gaatggatat
     7681 ttccctggcc gatcatgaaa tcaggaccat cctggcgcgc ctggaggaca tcccggaaga
     7741 ccagcggact gagtccggta tttcttcctc cgcggcgatg gagattatca gcaacgtgag
     7801 cgaaaaccgc caggtgaccg tccctgctga actgctggcg tccctgattc agaccgctga
     7861 acaggcattg tggaagcgtg aatgggccgc ccgtgattac ggcctcgccg tcccggaatg
     7921 cgttacccgc cgtcaggcgg tggttaatca ggcccgcatc ctgctgaaaa acaacacgca
     7981 cgaaaacgac tgatgttatc gccgcctccg ggcggcgatt cagggagacc atgaatcatg
     8041 aataaaacgc tgaatgcact gatttgtcgt catgcccgcg acctgctgct ggcgcagggc
     8101 tggccggaag agacggatgt tgaccagcgt aacctgaagt atccgggctg gatcagcatt
     8161 tacgtgcggc tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cgatggcgta
     8221 ctgccgccgc acctggcctc cgccattcag aaactgaccg gaaccggggc ggaacttggt
     8281 ttcacgccag cccacgaacg tttccacctg gcattatgtt cgccgggaga cgtctctcag
     8341 ctctggatgc tggttctggt gaattgtggt ggacagcctt tcgccgtcgt tcaggtgcaa
     8401 catatcttca cgcctgtcgc tatcagtcac acgctggcgc ttgccgcgac acttgatgcg
     8461 cagggataca gtgttaacga catcatccat atcctgatgg cagaaggagg tcaggcatga
     8521 gcgcacgttc acgggcactg attcccctca gcgcagaaca acaggccgcc atgcaggctg
     8581 tggctgtcac agaacaacgt cgtcgtcagg gacgcacact ttcagcatgg ccttatgcca
     8641 gcgctttctt tcgctgcctg aatggcagtc gccggatttc gctgaccgat ctccgctttt
     8701 ttgcccctgc gctgacgaag gaggaatttc atggcaaccg tctcctgtgg ctggctgccg
     8761 tggataaact gattgaaagt tttggtgaag tctgtgttct tcccctgcca tccgatgcgg
     8821 ggcatcgtct gttcccgtcc gttccttttc gtgaaggtga gcg
//
LOCUS       DAZFRJ010000174         8854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000174 DAZFRJ010000000
VERSION     DAZFRJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.176"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKUN_003795"
     CDS             complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKUN_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO6661974.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKUN_003796"
     CDS             complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKUN_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO6661975.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQVKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKUN_003797"
     CDS             4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKUN_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO6661976.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKUN_003798"
     CDS             5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKUN_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO6661977.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         2133 a         2270 c         2312 g         2139 t
ORIGIN      
        1 tcacgccgca tccggcatga acaacgcgca ctttgtcagt aatctcgggg caactaaatg
       61 tcgccccctt tggttattac cctaccgctg gcgtcgggtc atcgcctttg tagtcgcgtt
      121 tgacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc tggttaaagg
      181 caatgttgat gtcgttttca cggcacagct gatcgatagt acggttcagc tcatcgacag
      241 tacgactacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc aacgtgctgg
      301 caccaaacgc cgtaaagaag acttccggca ttggttcgtg catcaccctt gggtgctcag
      361 tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag gccacgccga
      421 gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa
      481 caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac
      541 gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta tcgccaatac
      601 gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa atttcttgta
      661 aaccaaaacc aagacctacg gataatgctg cggccagcca ctggagttta tcccaagaga
      721 cgcccagcga tccgaacacc gtcatcgcac caacggcaat aatgatgtag ttaaggatgg
      781 tagtaatggc atacgacgcg ccctgacgca tattcagtcg cgagagcacc agcacttcca
      841 gtaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata atcgcaaaca
      901 acagactgcc catggtgacg tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat
      961 gccagagcgt gatgctgtcg agatagctga acacggtgat caaatcggac caaattgccc
     1021 agaacatgac accgaacagc gcaaacatca gcaacatggt aatacgcagc gtctgctggt
     1081 taacctgttc cagtgcaatg gtgggttctt ccggcggttc ggcaccttct gcgccctctt
     1141 tcaccagatt ctgccgacgc gccaacgcac gacgccaggc gatacgccgc gccgctacgc
     1201 ttaagccgcg caataccgtc tggtagagca agttccagat gatcaccaga taaacggttt
     1261 caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggca gtcagcacca
     1321 tcaatgcaat cgggataatc gacagcacgg taatggtgac cagtcgcatg gtgtgcgact
     1381 ctttatcacg ccagctttcg cggcacattg gccataccag gaaggcaatc agcagcaggt
     1441 tgaagaaaat catcgcctgc cccagcacat catccatcag atgcagcggg gaaagttctg
     1501 ccaccacaga ccagaaatgg ataggcagca acgcgagact gatgcggaca atttgccgac
     1561 gccagtggct ggtctgctgc tccggcatgc cgaagtgacg cacggcaacg ccatttttct
     1621 ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt ttactgaacg
     1681 accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca accgcgagaa
     1741 taatcaggca caccggcagc gcgcggatca ggtcgataag gatcgctttt ggtgtgttga
     1801 gctggctatc gttacgcagg gaacccacag cggaagccag tttctgttga tacgctttca
     1861 gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa ccggcgagga
     1921 aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc atcgacttaa
     1981 attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt ggacggttac
     2041 tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgat acgctcatta
     2101 actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc aactgtttgt
     2161 tgagttgatc cagcaattcg cgacgcatat caaccacttg caataacgca tcgtgaactt
     2221 cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca tcgctctgga
     2281 agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt
     2341 tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg taaaggatac
     2401 gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg cgttccgatt
     2461 gcagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac tgattaccgt
     2521 tttcagtcgc ggtaatcaga cgctgactta actgctggtt aatttccagt tcctgtttca
     2581 ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc tgcgccgttt
     2641 tttcagttaa agtcaggcgc ttgctgttta ccgcttcttg caacagctgc aactggtgct
     2701 ccagacgggc gctgttcgcc gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga
     2761 cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc aacgcctgct
     2821 gagcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca tcagtcccat
     2881 ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc
     2941 gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga tcgttttgtg
     3001 cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg
     3061 tactcagaat tttgcgcgtt tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg
     3121 cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgtcgtagc tgaactgtct
     3181 cttcttttat gcgatcgatt ttatcgaggg tggctaatgt atctgtcaga tcctgctgca
     3241 ccagtttgtc ctgagcagaa agatcttttt gtttatttag tgagtcaagt tgcgcctgca
     3301 ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc
     3361 ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt
     3421 aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa
     3481 gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga aaatgtccag
     3541 gaaaaatcct ggagtcagat tcagggttat tcgttagtgg caggattacg aagcgtgggg
     3601 cacaggagat acatctccag taagatggca acgtaatcgc gggcttcttt tttaagatca
     3661 aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc gcgcataata
     3721 attgctgcgc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat acaatgtttt
     3781 aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc ctgttgcaca
     3841 acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa taatcgacgc
     3901 cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa tactgagagt
     3961 ggatcgccag ggaattttac ctgatactca agctctagtt caccaatatt ggattctgac
     4021 agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta gattgcaccg
     4081 cgcgtaacgc cagctgcttt tgcaatctca cccagcgagg tggatgatac cccctgctgt
     4141 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
     4201 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
     4261 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac ttttgaccat
     4321 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggtttacgc
     4381 ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt gacgacaaac
     4441 aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc aaaactgaac
     4501 ctctgcagat cacaaccgag cttccgggtc gcaccagtgc ctaccggatc gcagaagttc
     4561 gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc gacatcgaag
     4621 ctggtgtctc tctctatcag attgatcctg cgacctatca ggcgacatac gacagtgcga
     4681 aaggtgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt
     4741 atcagaaact gctcggtact cagtacatca gtaagcaaga gtacgatcag gctctggctg
     4801 atgcgcaaca ggcgaatgct gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga
     4861 tcaatctggc ttacaccaaa gtcacctctc cgattagcgg tcgcattggt aagtcgaacg
     4921 tgacggaagg cgcattggta cagaacggtc aggcgactgc gctggcaacc gtgcagcaac
     4981 ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg
     5041 aactggcgaa tggcacgctg aaacaagaga atggcaaagc caaagtgtcg ctgatcacca
     5101 gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt accgttgatc
     5161 agaccactgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac actctgctgc
     5221 cgggtatgtt cgtgcgcgca cgtctggaag aagggcttaa tccaaacgct attttagtcc
     5281 cgcaacaggg cgtaacccgt acgccgcgtg gtgatgccac cgtactggta gttggcgcgg
     5341 atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat aagtggctgg
     5401 tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc
     5461 ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa gccgcaagcg
     5521 gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga catgcctaat
     5581 ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg
     5641 gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc gccggcagta
     5701 acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac ggtgacacag
     5761 gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc taacagtgac
     5821 tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc ggatatcgcg
     5881 caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca agaagttcag
     5941 cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt cggcgttatc
     6001 aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc gaatatgaaa
     6061 gatgccatca gccgtacgtc gggcgtgggt gatgttcagt tgttcggttc acagtacgcg
     6121 atgcgtatct ggatgaaccc gaatgagctg aacaaattcc agctaacgcc ggttgatgtc
     6181 attaccgcca tcaaagcgca gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg
     6241 ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct gacctctact
     6301 gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt
     6361 gatgtggcga agattgagct gggtggtgag aactacgaca tcatcgcaga gtttaacggc
     6421 caaccggctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct ggataccgct
     6481 gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt
     6541 gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg
     6601 ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca gaacttccgc
     6661 gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt
     6721 gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct cgccatcggc
     6781 ttgttggtgg atgacgctat cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa
     6841 ggtttgccgc caaaagaagc tacccgtaag tcgatggggc agattcaggg cgctctggtc
     6901 ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg cggttctact
     6961 ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct gtcggtactg
     7021 gtagcgttga tcctgactcc ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc
     7081 gatcacgggg aaggtaaaaa aggcttcttc ggctggttta accgcatgtt cgagaagagc
     7141 acgcaccact acaccgacag cgtaggcggt attctgcgca gtacggggcg ttacctggtg
     7201 ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc aagctccttc
     7261 ttgccagatg aggaccaggg cgtatttatg accatggttc agctgccagc aggtgcaacg
     7321 caggaacgta cgcagaaagt gctcaatgag gtaacgcatt actatctgac caaagaaaag
     7381 aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg tggtcagaat
     7441 accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggcga agaaaacaaa
     7501 gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt
     7561 ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt tgactttgag
     7621 ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa ccagttgctt
     7681 gcagaagcag cgaagcaccc tgatatgttg accagcgtac gtccaaacgg tctggaagat
     7741 accccgcagt ttaagattga tatcgaccgg gaaaaagcgc aggcgctggg tgtttctatc
     7801 aatgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa cgactttatc
     7861 gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg tatgctgccg
     7921 gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc gttctcggca
     7981 ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa tggcctgcca
     8041 tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg
     8101 atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac ggggatgtcc
     8161 tatcaggaac gcctctccgg caaccaggca ccttcactgt acgcgatttc gttgattgtc
     8221 gtattcctgt gtctggcggc gctgtacgag agctggtcga ttccgttctc cgttatgctg
     8281 gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat
     8341 gacgtttact tccaggtagg cctgctcaca accattgggt tgtcggcgaa gaacgcgata
     8401 cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct gattgaagcg
     8461 acgcttgatg cagtgcggat gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc
     8521 ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca gaacgcagta
     8581 ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt cttcgttccg
     8641 gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat cgagcacagc
     8701 catactgtcg atcatcattg atacaacgtg taatcactaa ggccgcgcaa gcggcctttt
     8761 ttatgcataa cctacgaaca ttaaggagta attgaaccac caactcagga tctcatacga
     8821 aaaccagtat taaccacgga taaaattcat aaaa
//
LOCUS       DAZFRJ010000175         8730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000175 DAZFRJ010000000
VERSION     DAZFRJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.177"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..643)
                     /locus_tag="ACHKUN_003799"
     CDS             complement(<1..643)
                     /locus_tag="ACHKUN_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6661978.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQ"
     gene            800..1033
                     /locus_tag="ACHKUN_003800"
     CDS             800..1033
                     /locus_tag="ACHKUN_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6661979.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            1115..1822
                     /locus_tag="ACHKUN_003801"
     CDS             1115..1822
                     /locus_tag="ACHKUN_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6661980.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            1931..2593
                     /locus_tag="ACHKUN_003802"
     CDS             1931..2593
                     /locus_tag="ACHKUN_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO6661981.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            2590..2823
                     /locus_tag="ACHKUN_003803"
     CDS             2590..2823
                     /locus_tag="ACHKUN_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661982.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            2810..3718
                     /locus_tag="ACHKUN_003804"
     CDS             2810..3718
                     /locus_tag="ACHKUN_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6661983.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            3729..4607
                     /locus_tag="ACHKUN_003805"
     CDS             3729..4607
                     /locus_tag="ACHKUN_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6661984.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            4604..6004
                     /locus_tag="ACHKUN_003806"
     CDS             4604..6004
                     /locus_tag="ACHKUN_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6661985.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            6001..6258
                     /locus_tag="ACHKUN_003807"
     CDS             6001..6258
                     /locus_tag="ACHKUN_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6661986.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            6310..7299
                     /locus_tag="ACHKUN_003808"
     CDS             6310..7299
                     /locus_tag="ACHKUN_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6661987.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            7318..7698
                     /locus_tag="ACHKUN_003809"
     CDS             7318..7698
                     /locus_tag="ACHKUN_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6661988.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            7914..8111
                     /locus_tag="ACHKUN_003810"
     CDS             7914..8111
                     /locus_tag="ACHKUN_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6661989.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            8263..>8730
                     /locus_tag="ACHKUN_003811"
     CDS             8263..>8730
                     /locus_tag="ACHKUN_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6661990.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQ"
BASE COUNT         2183 a         2042 c         2370 g         2135 t
ORIGIN      
        1 actggatata gagcagcgcc attccctctt tttcgatgct ccatggctga tagttgggat
       61 tgtctgataa gaccatgatc ttgcttccaa ttttttgaag tcgtttgacg taacattcgc
      121 catcaaagca aaatgcataa atgccatcgc cgtcaaagta agttactgtt ttatctaaaa
      181 acagtaagtc tcccggggag attgttgggg ccatgctgtc ccctctggcg ttaccgattt
      241 ctatgttctt gaatgctctg ttaccgacca ggcggcgggc atattcagga tcaagttcta
      301 ttgagcgtac tacatcaatt agatctcccc gtacatgagt tccgtcgccg caactgaatt
      361 caacatccag tacattgaac acaacgctgt cttctcttgt atggtgtttt tcagctgaag
      421 agaatgtcga tgaggcatct tcacccaaaa accaggattg tggataacca ctaattactg
      481 atagctgtgc aagtttatcg cttctgggaa acgtctttcc tgtagtccag tattgtactg
      541 attgcgcgct tacacccagc ttacgggcta gttcagcctg actccagcct ttcgttttca
      601 gcatcattgc tatccgattt tctgtgcttt ttacattctt catggtgagt tcctgtcgaa
      661 gctttataca cataaatatt aacttgattt tagtgtattc gatccttttg aaacttgcat
      721 gttaatttat tcttgatgta ttcttgattt gtaaagttaa tgttggagct gcgctgtgat
      781 aaaactgaat gattacgaca tgttgcgtaa attgatccct caaaacgcca tagcgagata
      841 catcggtgtt acgccacaag cggtgaatct gtggttttct aaaaactccg ttccttctcg
      901 ttttgtttta cgggtgtgtg aattggttga atggaaggtt actccgcatg ggttaagacc
      961 tgatctatac ccttatcccg aagatggaat tcctgattcg ttacgaaaat caaacggtat
     1021 taccagagat taaagactga ctgtcacaag cagtaattca cgatgaatac gttttggcgt
     1081 gagttctgaa atgtcatcct caaggagcaa tcagatgaac accgcaattt ttaacgataa
     1141 agcatccatg accagcgttg agattgcaga gctggtgggc agccaacaca aagatgttaa
     1201 acgcagcatt gaaagactca tggggaaggg agttatccga agtgcgccaa tggcgaattt
     1261 cgaaataatc aacaacttag gattgaaaag aaatgtagct gcttacgtat tcgaaggtga
     1321 acaaggcaaa cgcgacagta tcattgttgt cgcgcagatt tgccctgaat tcaccgcccg
     1381 cttggtagac cgctggcgtg aactggaaga acagatccgt aagccaatga gcgaaatcga
     1441 aatggttgcc gcgatggctc ttgaagccgt tcgccaacag aaacggatca ctcaggtgga
     1501 agaaaaagtc atccacgttg ctgaaacagt tgagcaaatt aaaaagggca ctattcgtga
     1561 gggctatgcc ggatatcgcc aactgaaagc aaaaaccggt ttgtcagatg ataaatgctg
     1621 caatctggtg aacgcctatc aaattcctac agacacacat gagttcatga cgccggacgg
     1681 attgttgtca cgtcgcgcaa ttgttgctgt ggaaccgttt atggctgctt tttatcgggt
     1741 tatggaggaa gcagaaccgc gagggactcg ctggtatcac ccgaaaatgg ggttatttca
     1801 ggttattggt tggcagcggt gaaaaaaagc cgggagtaac ccggctcact caacatcaat
     1861 aacggggagc tgtttcgcat aaaacggctc cgaaacatcc aagaacagtt ctaaagatat
     1921 cagcagctat atgatcattt caagaccaaa tattgattct gcaatttcgg gacgttacac
     1981 tgtctccgca ccttataaag cgggtgccgg gggtcgcagc ccggaattgc tcgaggcgat
     2041 atacgacgcg ccagcgtctt ttttatcgtc catgctcgcg cacgccagaa ttatggtggg
     2101 ctgggcaggg gagccgaaag gctcgccggt ttccttgagc gccggtactg cgaaccctgt
     2161 tcagttcacc acccatgagt ttcgcagctc cggtggtgga agtttttcac tgctcaagga
     2221 ggctgccatc atggctacag tcccaacttc cccatacctg aaaattgaag ttgtcaacgg
     2281 caaggccgtt attttctccc tgcatgttgc ctgccacttt aagcgcatgc accagaacat
     2341 cgtcgacaaa atcgagtatc tgaactgctc acgcgagttt tttacccgca atttcatacc
     2401 gggtacttat cacatctacg gtgactccct gcgtggttat tacatcaccc ttgatggtct
     2461 gatgatgctt cagcttgggt taagtctacg cacaatgcgg tactacgaga gctgcattga
     2521 agcattccat gaggcagaaa tcagcctcag tcacaccgct ttccgccgta atcaatggga
     2581 ggtgcgccca tgattcgccg cgtcgttaat tccctgtatc accgatacaa ccgttgcccc
     2641 cgtgtggggc agtggttcac caccagcaac ggccacgttc tgcgggtttg cctggtcaat
     2701 acagaaagcc agatggttgt ctgccaggtt cagggacgta ctcataccct gagttatccg
     2761 ctggtggctt ttcagtccgg gaaaatgttt aagcgtctgg gaggtggcta tgcgtccgtc
     2821 tgatcttctg ctcgattttg ggcatccggt tgcttattac cctgggctcg ttaaatacat
     2881 gggaagtccg cacgctgtta ttttctttgg tcagattttt tactggcagg ataaagcaca
     2941 tgcagcggaa ggcgtacata aaacgcgtga agagatacaa cacgaaactg gacttacatt
     3001 tgaacaacag gctgtagcgc gtaagcatct tgtgtccaga ggcattttgg ttgaaaccaa
     3061 caagcgtctt gagcacaaaa tgttctaccg tatagattgt gagcgcctta atgaaattat
     3121 caatgaaaac aatcagtttt cccgaaatgg ggaaacccgt tttcgggaaa ctgtaaaacc
     3181 caatttcgcg gaggagggaa agccttcacc gcggacacgg gaaaccactc gccgcggaga
     3241 agggaaaacc aatttcgatc ttacagagaa tacaacagag attacttcag agaatactac
     3301 agagagtaaa aacactattg gcgcatccgc tgacgcgtct gcaccagcgc gttctgcccg
     3361 acaggaatat tcaccggaat ttgaacaggc ctggcaggaa tatcccaaac gtgctggtgg
     3421 taattccaag tcagcagcct tcaaagcctg gaaagcccga atcagggaag gtgtgacacc
     3481 agaaaccatg ctcaacggtg tgaaacgcta tgctgcctgg gtgcgtgtct caggaaatac
     3541 cggcacccag ttcgtgaagc aggcgtcgac gttctttgga cccgatcgtc acttcgaaga
     3601 tttctggcaa cagccagcag ctcccggagg tgggcgacag cgacaggtcg atgtcctggc
     3661 tggcctggga gccatgtctg acaaattcgg taaatccagt gacaaattga cattctgagg
     3721 tgacagcgat gatgacgttt aacctgcgtg aacaacaaac aagactacag gcgcggatgg
     3781 atgagttacg ggcagagatt gcatttgctc agaagggcga aaaaccatgg ccttatcgtt
     3841 cctgctggat gcgtgaaggg cgcgggtgtt gcgaaaaaca tggcgaatac cacacacata
     3901 ttctggtgtg gagcgatcgt aatggcgagg acagagaaaa aatttcatgc tgccctgact
     3961 gcttgatagc tgaggccaac gatttgacca tggagctgtc gtccctcaag gcggaagaac
     4021 tgactgataa cgccggaatt gctctgcgtt ttcgggactg cgagtttgat aattatctgg
     4081 aggttaatcc tgacgcagcc agaaatcttg cggcctgtcg ccgctatgcg gagaactggc
     4141 cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgcggt accgggaaaa
     4201 atcatctggc ggtatcaatg gcaaaacaca tcatccgtaa ctatctggcc agtgtggaga
     4261 tcaccgacgt gatgcgcctt acccgtgctg tgaaaaactg ctggcggaat gacagtgaaa
     4321 aaacagcgga tgacgtcatt gagcattatg cgtcactgga tttgctgatc atcgacgaag
     4381 tcggcgttca gtttggcagt gcggctgaaa tggccatttt gcaggaaatt atcaatgccc
     4441 ggtatgaggg tattttgcca actatcttga tcagcaacct ttcaccggaa gaattgtggg
     4501 cgttcatcag tccccggatt gccgacagga tcaccaatgg cgggcgcaac tggttgtcgt
     4561 ttaactggcc cagctaccgt tctcgtatcg gaggtgttgc cgcatgacca gccagaacac
     4621 cccggcatgg cgtaacgatg acctggaagg cgctgtcatt ggcgcgtttt ttctgcgtgg
     4681 ggctgatccg gaagtgatgg atattctggc cacattgtcg gcggacgttt tttctgcacg
     4741 acagtaccgg gatatttacg cgggaatttg cagacaggca cgtgtatccg gcgtcattga
     4801 ccccgtactg ctgtgcaatg agatgccgga acttgccccg gtgattaccg acaccggacg
     4861 caaaacctgg gtgaagtctt cactggagca ctatgtcgca gcgttgcggc gtaatgccgc
     4921 actgcgcgat gcagaaaaaa cactgactga agcattacag aatttacggg atgcgcatac
     4981 ctgtgaagca gccgaggatg ccctgaagga tgcgcagaac atgatggcct cactgtcgac
     5041 cggaaagggc gtcattcagc cggttcacat tgatgatgtc cttccggaag tggtcgaccg
     5101 tgttgaatgc cgcaatcagg gactggagaa atccagggcg ctgatgaccg gtattgatga
     5161 actggacgca aaaacgggcg gtatggagcc aggcgacctg gtattcattg cggcccgtcc
     5221 ttcgatgggg aaaaccgaac ttgcgctgga tatcatcgac aaggtgactg agcaggggca
     5281 tggcgtgctt ctgttcacca tggagatggc gaacattcag attggtgaac gtatggtgtc
     5341 tgctgccggg ggaatgccgg tatcccgtct taagtctgtt gcccgttttg aagatgaaga
     5401 ctgggcgcgt ttctcacagg gcgtgggacg aatgacgggg cgtaatatct ggatggtgga
     5461 ccaggcaaac ctgaccattg atgagatatg tgcaaccacg aggcaccacc ggatgaaaca
     5521 cccggaaacg gcgctggtgg tggtcgatta cctcggcctg attaaaaccc gcagcacggg
     5581 gcgtcacgac cttgctgtgg gggaaatctc aaagggactt aaaagcctgg caaaatccgg
     5641 cggttttccg ctgattgctc tgagccagct ctcccgtggc gtggaatcca gacccaataa
     5701 acgccccatg aactcggacc tgaaaaactc aggggaaata gaggcggatg cagacatcat
     5761 tctgatgctt tacagggatg aggtgtacaa cccggagaca caggcgagag gcatagctga
     5821 aatcaacatc acgaaacagc gtaatggcac gctgggtacc atttaccggc gttttcataa
     5881 cgggcatttt ctgcctgtgg accaggagag tgcccgggtt ctttccactc ccatgacgcc
     5941 gggcaatccg cgcagataca gcaataaccg catgtcgggt agtaaaacgg agcgtttatt
     6001 ttgaacaaca gaaccaccac tgtttcaccg gaacaacttc gtcggcaggc gcaggagatg
     6061 cttcgttgtg ctgaacagat ggaaaaaacg agcgtggcaa aagatacgct ccgcaagcag
     6121 cttactccgg cgcttcgtga tctgctgcag gcaaaacatc gcacacaaaa ggcggtggat
     6181 gagctggtgg attgcgtggc ggaactggaa ggccaggtaa gccagtttga aacgctggtg
     6241 aaagagttta ctgcgtgatg gctgaatttt ttctcctgcg ttcatgcaat accgttcgct
     6301 gaggtgaccg tgagagcact gctgacccct gaaattgccc cgcgtatggg gatcgtcttg
     6361 ttcagacccg gttcagagct gatgcccctg tttatgcagg ggcgtgtcct gctggagcct
     6421 gagccggaac gttattcatc ttttgccagt ggtgccgttc cggcagcatc acaaccgctg
     6481 gcggatgatc ctgccgttcg ggccgtgttc cgccatgagg cggtgatccg tcgtgctggt
     6541 ggcgtggaat gccttgagag ctggttactt cgtgaaaaag gctgccagtg gcctcattcc
     6601 ggatggcaca gcgagaacat gaccacaatg cgacacgcgc cgggcgcaat ccgtctgtgc
     6661 tggcactgcg ataacctgct tcgcgatcag ttcacggaac gtctggagtc aatggcaacg
     6721 gataactgtg cccgctgggt gttgtccgta gtccgtcgtg atctcggttt tgatgacagt
     6781 cacgttgtga caatgcctga actgtgctgg tggctggttc gtaatgacct ggcggatgcc
     6841 ttaccggaaa gtgcagcccg taaggccctg agattaccga agcctgtttt gccgtctgtc
     6901 acccgggaga gtgaccttgt gccttcggtt cctgccacca gcattatcca ggataaagcg
     6961 aaaaaggtgc tggcgctgaa agtggatccg gagtcgccgg agtcttttat gttacgcccc
     7021 aaacgtcgtc gctgggttaa tgaaaagtac acgcgctggg ttaagacaca gccttgcgcg
     7081 tgttgtggta agccagccga cgatccacat cacctgattg gccatggtca gggtggaatg
     7141 ggtacaaaag cgcatgacct ttttgtgttg cctttgtgca gaaaacacca tgacgaactg
     7201 catgcggata ccgtggcatt tgaagagaag tatggttccc aactggagct gatatttcgt
     7261 tttatcgatc gcgcactggc gattggtgtg ctgtcctgat ttttgtggag aaagttgatg
     7321 cgtgatattc agatggttct tgaacgctgg ggggcatggg cggcaagtgg taacaccggg
     7381 gtggactatt ctccgatcgc tgccggattc aaaggccttt taccatctac cactaaacca
     7441 cgtccagcct gctgcgatga tgacggactt atcattgaaa actgtcttgc acgtctgaag
     7501 cagaaaaaac ctgaggagta ttcgcttctc attgctcatt atttgttgcg aatatcaaaa
     7561 agacagatag ccaggacgag aaagaaaagc gaaaaagcaa tacgaattga gatgcagata
     7621 gccgaagggt ttattgacgg atgtttgtct gtgctggggg taagactgga gatggacgac
     7681 tggctgctaa aaaagtaaaa aatgattagt gcggtccgca aaaagtatgt cagtatgtta
     7741 agagtggtta ctttgccaca cagcttaaat ccgccgtcag gcgggttttt tatgcctgaa
     7801 atcggaccag tacgttaaac gcgctggtgg cggtgaatac cggtctttca gcttgctggc
     7861 tttttcgaca agagttattg gtgtgtcacg ttaaccggaa aagggaaaaa gacatgctga
     7921 aacagcagga tatgacagaa accgccagag tggtgtttaa tgaattaagt gccaccgcac
     7981 cggcgacagt cggggagatt gcacagaata cgtacctttc acgcgaacgc tgccagctaa
     8041 tactgaccca gcttgttatg gcgggtctgg cagactatca gtttggttgt tacagacgcc
     8101 ttcagtcctg aaggcttttt tatttgtggt aaatgggcgg ctggtgggtg ttaggggcac
     8161 tcaccagcca tctgctcatg cgttggggtc acaagcaaac ctcaggccca tctgctttgc
     8221 gcaaaagcgg tatgagccta tcagagacag gcttaatgat ccatgtttaa tactgtaaaa
     8281 atatccagtt gtgagttaat caacgccgac tgcctggaat ttatccggtc gttacccgaa
     8341 aattctgttg acctgatagt cacggacccg ccgtacttta aagtgaagcc tgagggctgg
     8401 gataaccagt ggacgggtga tgaggattac ctgaaatggc tggaccagtg tctggcgcag
     8461 ttctggcggg tgctgaaacc tgccggaagt ctttacctgt tctgtggcca tcgcctggca
     8521 tctgacattg aaatcatgat gcgtgaacgc ttcagtgtgc tgaaccatat tatctgggcg
     8581 aagccgtccg gacgctggaa cgggtgcaac aaggaaagcc tgagggcgta tttccccgcc
     8641 acagagcgca ttctgttcgc ggaacattat caggggccgt atcgtccgaa agatgccggg
     8701 tatgaggcga agggcagggc actgaaacag
//
LOCUS       DAZFRJ010000176         8711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000176 DAZFRJ010000000
VERSION     DAZFRJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.178"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(350..613)
                     /gene="rpsT"
                     /locus_tag="ACHKUN_003812"
     CDS             complement(350..613)
                     /gene="rpsT"
                     /locus_tag="ACHKUN_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO6661991.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            716..934
                     /locus_tag="ACHKUN_003813"
     CDS             716..934
                     /locus_tag="ACHKUN_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO6661992.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            942..1883
                     /gene="ribF"
                     /locus_tag="ACHKUN_003814"
     CDS             942..1883
                     /gene="ribF"
                     /locus_tag="ACHKUN_003814"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO6661993.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1926..4742
                     /gene="ileS"
                     /locus_tag="ACHKUN_003815"
     CDS             1926..4742
                     /gene="ileS"
                     /locus_tag="ACHKUN_003815"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO6661994.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4742..5236
                     /gene="lspA"
                     /locus_tag="ACHKUN_003816"
     CDS             4742..5236
                     /gene="lspA"
                     /locus_tag="ACHKUN_003816"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO6661995.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHKUN_003817"
     CDS             5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHKUN_003817"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6661996.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5775..6725
                     /gene="ispH"
                     /locus_tag="ACHKUN_003818"
                     /gene_synonym="lytB"
     CDS             5775..6725
                     /gene="ispH"
                     /locus_tag="ACHKUN_003818"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO6661997.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6791..7705
                     /gene="rihC"
                     /locus_tag="ACHKUN_003819"
     CDS             6791..7705
                     /gene="rihC"
                     /locus_tag="ACHKUN_003819"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO6661998.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7872..8693
                     /gene="dapB"
                     /locus_tag="ACHKUN_003820"
     CDS             7872..8693
                     /gene="dapB"
                     /locus_tag="ACHKUN_003820"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO6661999.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1987 a         2158 c         2478 g         2088 t
ORIGIN      
        1 tttacaagat ctaataagga gatatttatg aatggttaaa gtgtatagga atttaattca
       61 aaaatagagt ggtcttaagt ttaatacctc aatgtgtatc acaatatcca cattctttgt
      121 aggggagtct ggagattgag tagatattct tgttcagaat gtatcagccg atggttctac
      181 gattcttaag ccacgaagag ttcagaaagt gtaacggcat gtctgcaaaa gccagcatct
      241 tttgactatc tggcaaccgg tagtgtgttc tctcacgcat cataaaagca gcaggcataa
      301 aaaaacccgc ttgcgcgggc tttttcacaa agcttcagca aattggcgat taagccagtt
      361 tgttgatctg tgcagtcagg ttagccttat gacgtgcagc tttgtttttg tggatcagac
      421 ctttagcagc ctgacggtcc acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag
      481 ctttgtcgcc agcttcgata gctgcgtata ctttcttgat gaaagtacgc atcatagagc
      541 gacggcttgc gttgtgctta cgagcctttt cagactgaat ggcgcgcttc ttagctgatt
      601 tgatattagc caaggtccaa ctcccaaatg tgttctatat ggacaattca aaggccgagg
      661 aatatgccct tttagccttc ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg
      721 ccggcactcg ttacgtagtg atggcgcagg attctaccag cttgcagggt gtgaatacag
      781 cttttccgcg ataaaaattg cagcaggcgg tcactttctt cccgtgattt gcgccatggc
      841 aatgaaaagc cacttctttc tgatttcggt actcaatcgc cggttaacct tgaccgctgt
      901 acaaggtata ctcggacgat tttcactgtt ttgagccaga catgaagctg atacgcggca
      961 tacataatct cagccaggcc ccgcaagaag ggtgtgtgct gactattggt aatttcgacg
     1021 gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca ggaagaaggg cgcaagcgca
     1081 acttaccggt gatggtgatg ctttttgaac ctcaaccact ggaactgttt gctaccgata
     1141 aagccccggc acggctgacc cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg
     1201 ttgattacgt gctgtgcgtg cgtttcgaca ggcgtttcgc ggcgttaacc gcgcaaaatt
     1261 tcatcagcga tcttctggtg aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt
     1321 tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt acagaaagct ggcatggaat
     1381 acggcttcga tatcaccagt acgcaaactt tttgcgaagg tggcgtgcgt atcagcagca
     1441 ccgctgtgcg tcaggcgctg gcggatgaca atctggctct ggcagaaagt ttactggggc
     1501 acccgtttgc catctccggg cgtgtagtcc acggtgatga attagggcgc actataggtt
     1561 tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg
     1621 tagaagtgct gggcctcggt gaaaagccgt tacccggcgt ggcaaacatt ggaacacgcc
     1681 caacggttgc cggtattcgc cagcagctgg aagtgcattt gttaggtgtt gcaatggacc
     1741 tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg
     1801 cgtcgctgga cgaactgaaa gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt
     1861 ttgggctaac aaaaccggct taagcctgtt atgtaatcaa accgaaacac ggaaccgaga
     1921 atctgatgag tgactataaa tcaaccctga atttgccgga aacagggttc ccgatgcgtg
     1981 gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg gactgatgat gatctgtacg
     2041 gcatcatccg tgcggctaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt
     2101 atgcgaatgg cagcattcat attggtcact cggttaacaa gattctgaaa gacattatcg
     2161 tgaagtccaa agggctttcc ggttatgact cgccgtatgt gcctggctgg gactgccacg
     2221 gtctgccgat cgagctgaaa gtagagcaag aatacggtaa gccgggtgag aaattcaccg
     2281 ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca
     2341 aagactttat ccgtctgggc gtgctgggcg actggtcgca cccgtacctg accatggact
     2401 tcaaaactga agccaacatc atccgcgcgc tgggcaaaat catcggcaac ggtcacctgc
     2461 acaaaggcgc gaagccagtt cactggtgcg ttgactgccg ttctgcgctg gcggaagcgg
     2521 aagttgagta ttacgacaaa acttctccgt ccatcgacgt tgcttttcag gcggtcgatc
     2581 aggatgcact gaaagcaaaa tttgccgtaa gcaacgttaa cggcccaatc tcgctggtga
     2641 tctggaccac tacgccgtgg actctgcctg cgaaccgcgc aatctctatt gcacctgatt
     2701 tcgactatgc gctggtgcag atcgacggtc aggccgtgat tctggcgaaa gatctggttg
     2761 aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg
     2821 cggagcttga gttgctgcgc tttacccatc cgtttatggg cttcgacgtt ccggcaatcc
     2881 tcggcgatca cgttaccctg gatgccggta ccggtgccgt tcacaccgcg cctggccacg
     2941 gcccggacga ctatgtgatc ggtcagaaat acggcctgga aaccgctaac ccggttggcc
     3001 cggacggcac ttatctgccg ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag
     3061 cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa
     3121 tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacgc
     3181 cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc gcagtcactg aaagagatca
     3241 aaggcgtgca gtggatcccg gactggggcc aggcgcgtat cgagtcgatg gttgctaacc
     3301 gtcctgactg gtgtatctcc cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc
     3361 acaaagacac ggaagagctg catccgcgta ctctcgaact aatggaagaa gtggcaaaac
     3421 gcgttgaagt tgacggcatc caggcgtggt gggatcttga tgcgaaagag atcctcggcg
     3481 atgaagctga tcagtacgtg aaagtgccgg acacattgga tgtatggttt gactccggat
     3541 ctacccactc ttctgttgtt gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt
     3601 atctggaagg ttctgaccag caccgtggtt ggttcatgtc ttccctaatg atctccaccg
     3661 cgatgaaagg caaagcgccg tatcgtcagg tactgaccca cggctttacc gtggatggtc
     3721 agggtcgcaa gatgtctaaa tccatcggca ataccgtttc gccgcaggat gtgatgaaca
     3781 aactgggcgc ggatattctg cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg
     3841 ccgtttctga cgagatcctg aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg
     3901 cgcgcttcct gctggcaaac ctgaacggtt ttgatccagc aaaagatatg gtgaaaccgg
     3961 aagagatggt ggtactggat cgctgggccg taggttgtgc gaaagcggca caggaagaca
     4021 tcctcaaggc gtacgaagca tacgatttcc acgaagtggt acagcgtctg atgcgcttct
     4081 gctccgttga gatgggttcc ttctacctcg acatcatcaa agaccgtcag tacaccgcca
     4141 aagcggacag cgtggcgcgt cgtagctgcc agactgcgct gtatcacatc gctgaagcgc
     4201 tggtgcgctg gatggcacca atcctctcct tcaccgctga tgaagtgtgg ggctatctgc
     4261 cgggcgaacg tgaaaaatac gtctttaccg gtgagtggta cgaaggcctg tttggtctgg
     4321 cagacagtga agcgatgaac gatgcgttct gggacgagct gttgaaagtg cgtggcgaag
     4381 tgaacaaagt cattgagcaa gcgcgtgccg ataagaaagt gggtggctcg ctggaagcgg
     4441 cagtaacctt gtatgcagaa ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat
     4501 tacgatttgt cctgttgacc tccggcgcta ccgttgcaga ctataatgac gcacctgctg
     4561 atgctcagca gagcgaagta ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg
     4621 agaagtgccc acgctgctgg cactacaccc aggatgtcgg caaggtggcg gaacacgcag
     4681 aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct
     4741 gatgagtcaa tcgatctgtt caacagggct acgctggctg tggctggtgg tagtcgtgct
     4801 gattatcgat ctgggcagca aatacctgat cctccagaac tttgctctgg gggatacggt
     4861 cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt
     4921 ccttgccgat agcggcggct ggcagcgttg gttctttgcc ggtattgcga ttggtattag
     4981 cgtgatcctg gcagtgatga tgtatcgctc gaaggccacg cagaagctaa acaatatcgc
     5041 ttatgcgctg attattggcg gcgcgctggg caacctgttc gaccgcctgt ggcacggctt
     5101 cgttgtcgat atgatcgact tctacgtcgg cgactggcac ttcgccacct tcaaccttgc
     5161 cgatactgcc atttgtgtcg gtgcggcact gattgtgctg gaaggttttt tgccttctaa
     5221 agcgaaaaaa caataataaa ccctgccgga taaggcctac agattgctgc gaaatcgtag
     5281 gccgcatccg gcaaaaatcc tcaaaatata agagcaaacc tgcatgtctg aatctgtaca
     5341 gagcaatagc gccgtcctgg tgcacttcac gctgaaactc gatgatggca ccaccgctga
     5401 gtctacccgc aacaacggta aaccggcgct gttccgcctg ggcgatgctt ctctttctga
     5461 agggctggag caacacctgt tggggctgaa agtgggcgat aaaaccacct tctcgctgga
     5521 gcccgatgcg gcgtttggcg tgccgtcacc ggacctgatt cagtacttct cccgccgtga
     5581 atttatggat gcaggcgagc cagaaattgg cgcaattatg ctttttaccg caatggatgg
     5641 cagtgagatg cctggcgtga tccgcgaaat taacggcgac tccattaccg ttgatttcaa
     5701 ccatccgctg gccgggcaga ccgttcattt tgatattgaa gtgctggaaa tcgatccggc
     5761 actggaggcg taacatgcag atcctgttgg ccaacccgcg tggtttttgt gccggggtag
     5821 accgcgctat cagcattgtt gaaaacgcgc tggccattta cggcgcaccg atatatgtcc
     5881 gtcacgaagt ggtacataac cgctatgtgg tcgatagctt gcgtgagcgt ggggctatct
     5941 ttattgagca gattagcgaa gtaccggacg gcgcgatcct gattttctcc gcacacggtg
     6001 tttctcaggc ggtacgtaac gaagcaaaaa gtcgcgattt gacggtattt gacgcgacct
     6061 gtccgctggt gaccaaagtg catatggaag tcgcccgcgc cagtcgccgt ggcgaagaat
     6121 ctattctcat cggtcacgcc gggcacccgg aagtggaagg gacaatgggc cagtacagta
     6181 acccggaagg gggaatgtat ctggtcgaat cgccggacga tgtgtggaaa ctgacggtca
     6241 aaaacgaaga gaagctctcc tttatgaccc agaccacgct gtcggtggat gacacgtctg
     6301 atgtgatcga cgcgctgcgt aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca
     6361 tctgctacgc cacgactaac cgtcaggaag cggtacgcgc cctggcagaa caggcggaag
     6421 ttgtgttggt ggtcggttcg aaaaactcct ccaactccaa ccgtctggcg gagctggccc
     6481 agcgtatggg caaacgcgcg tttttgattg acgatgcgaa agatatccag gaagagtggg
     6541 tgaaagaggt taaatgcgtc ggcgtgactg cgggcgcatc ggctccggat attctggtgc
     6601 agaatgtggt ggcacgtttg cagcagctgg gcggtggtga agccattccg ctggaaggcc
     6661 gtgaagaaaa cattgttttc gaagtgccga aagagctgcg tgtcgatatt cgtgaagtcg
     6721 attaagtcat tagcagccta agttatgcga aaatgccggt cttgttaccg gcatttttta
     6781 tggagaaaac atgcgtttac ctatcttcct cgatactgac cccggcattg acgatgccgt
     6841 cgccattgcc gccgcgattt ttgcacccga actcgacctg caactgatga ccaccgtcgc
     6901 gggtaatgtc tcggttgaga aaactacccg caatgccctg caactgctgc atttctggaa
     6961 tgcggagatt ccgctcgccc aaggggccgc tgtgccgctg gtacgcgcac cgcgtgatgc
     7021 ggcatctgtg cacggcgaat cgggaatggc tggctacgac tttgttgagc acaaccgaaa
     7081 gccgctcggg ataccggcgt ttctggcgat tcgggatgcc ctgatgcgtg caccagagcc
     7141 tgttaccctg gtggccatcg gcccgttaac caatattgcg ctgttacttt cacaatgccc
     7201 ggaatgcaag ccgtatattc gccgtctggt gatcatgggt ggttctgccg gacgcggcaa
     7261 ctgtacgcca aacgccgagt ttaatattgc tgccgatcca gaagctgctg cctgtgtctt
     7321 ccgcagtggt attgaaatcg tcatgtgcgg tttggatgtc accaatcagg caatattaac
     7381 tcctgactat ctctctacac tgccgcagtt aaaccgtacc gggaaaatgc ttcacgccct
     7441 gtttagccac taccgtagcg gcagtatgca aagcggcttg cgaatgcacg atctctgcgc
     7501 catcgcctgg ctggtgcgcc cggacctgtt cactctcaaa ccctgttttg tggcagtgga
     7561 aactcagggc gaatttacct caggcacgac ggtggttgat atcgacggtt gcctgggcaa
     7621 gccagccaat gtacaggtgg cattggatct ggatgtgaaa ggcttccagc agtgggtggc
     7681 tgaggtgctg gctctggcgt cgtaacctgt cacatgttat tggcatgcag tcattcatcg
     7741 actcatgcct ttcactgata tccctccctg tttatcatta atttctaatt atcagcgttt
     7801 ttggctggcg gcgtagcgat gcgctggtta ctctgaaaac ggtctatgca aattaacaaa
     7861 agagaatagc tatgcatgat gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta
     7921 tgggccgcca gttgattcag gcggcgctgg cattagaagg cgtgcagctg ggcgcggcgc
     7981 tggagcgcga aggatcttct ttactgggca gcgacgccgg tgagctggct ggtgcaggga
     8041 aaacaggcgt taccgtgcaa agcagcctcg atgcggtaaa agatgatttt gatgtgttta
     8101 tcgattttac ccgtccggaa ggtacgctga accatctcgc tttttgtcgc cagcatggca
     8161 aagggatggt gatcggcact acggggtttg acgaagccgg taaacaggcg attcgtgacg
     8221 ccgccgccga tattgcgatt gtctttgccg ccaattttag cgttggcgtt aacgtcatgc
     8281 ttaagctgct ggagaaagca gccaaagtga tgggtgacta caccgatatc gaaattattg
     8341 aagcacatca tagacataaa gttgatgcgc cgtcaggcac cgcactggca atgggagagg
     8401 cgatcgccca cgcccttgat aaagatctga aagattgcgc ggtctacagt cgtgaaggcc
     8461 acaccggtga acgtgtgcct ggcaccattg gttttgccac cgtgcgtgca ggtgacatcg
     8521 ttggcgaaca taccgcgatg tttgccgata ttggcgagcg tctggagatc acccataagg
     8581 cgtccagccg catgacattt gctaacggcg cggtaagatc ggctttgtgg ttgagtggta
     8641 aggaaagtgg tctttttgat atgcgagatg tgcttgatct caatagtttg taaccacaaa
     8701 atatttgtta t
//
LOCUS       DAZFRJ010000177         8615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000177 DAZFRJ010000000
VERSION     DAZFRJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.179"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(251..1303)
                     /gene="fbaB"
                     /locus_tag="ACHKUN_003821"
     CDS             complement(251..1303)
                     /gene="fbaB"
                     /locus_tag="ACHKUN_003821"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO6662000.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1560..2837
                     /locus_tag="ACHKUN_003822"
     CDS             1560..2837
                     /locus_tag="ACHKUN_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO6662001.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2834..3838
                     /locus_tag="ACHKUN_003823"
     CDS             2834..3838
                     /locus_tag="ACHKUN_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6662002.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3835..4800
                     /locus_tag="ACHKUN_003824"
     CDS             3835..4800
                     /locus_tag="ACHKUN_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6662003.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4774..5520)
                     /locus_tag="ACHKUN_003825"
     CDS             complement(4774..5520)
                     /locus_tag="ACHKUN_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6662004.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5572..6390)
                     /locus_tag="ACHKUN_003826"
     CDS             complement(5572..6390)
                     /locus_tag="ACHKUN_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO6662005.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(6455..7255)
                     /gene="thiD"
                     /locus_tag="ACHKUN_003827"
     CDS             complement(6455..7255)
                     /gene="thiD"
                     /locus_tag="ACHKUN_003827"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO6662006.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7252..8040)
                     /gene="thiM"
                     /locus_tag="ACHKUN_003828"
     CDS             complement(7252..8040)
                     /gene="thiM"
                     /locus_tag="ACHKUN_003828"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO6662007.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8075..8171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8263..8535)
                     /gene="rcnR"
                     /locus_tag="ACHKUN_003829"
     CDS             complement(8263..8535)
                     /gene="rcnR"
                     /locus_tag="ACHKUN_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO6662008.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         1962 a         2243 c         2252 g         2158 t
ORIGIN      
        1 tgtcgagata acgatcacaa aagcgacaat gtgaaaattg ttcgaggagt aaaaggcaaa
       61 aaaaaacggc ctcccgatag ggaagccgta gcaaagtgcg cgtgttttta tgccggatgc
      121 ggtgtaaaca tacgtggcag tgctatgtag gcatgataag acgcttccgc gtcgcatcag
      181 gcataggttg ccggatgcag tgtaaacgcc ttatccgtcc tacggggtgg tgctgttcgt
      241 atgaggcgta tcaggcaata gtaattttgc tatcgagata aacgtcctgc acggcattaa
      301 tcagtttcac gccgtcagcc atcgatttct tgaacgcctt acgtccaaga atcagcccca
      361 ttccgccagc gcgtttgttg ataaccgcag tacgcactgc atcgctgagg tcagtttcac
      421 cgcccgcagc accgccggag tttatcaacc cagcccgacc catatagcag ttagctaact
      481 gataacgcac cagatcaatc gggttttcgc tggtcaattt gctgtaaaca cgatcgtcgg
      541 tgtaaccgta attaattgct ttatagccgc cgttattttc cgccattttt tgtttgacga
      601 tatctgcacc gatggttgcc gccagatggt ttgcctgacc ggtcaggtcg gcggaaacat
      661 ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa ataggcccac agcactgtca
      721 ccatacccag ctcgtgcgca cgttcaaaag ccgcagaaat ttcttcaatc tggcgacgtg
      781 actcttccga gccaaaatag atagtcgcac caaccgcaac cgcgcccatg ttgaacgcct
      841 gctccacgct ggcatacagc gtttgatcgt aggtattcgg gtaacttagc gtctcgttgt
      901 gattaagttt gacgaggaat ggaatgcgat gcgcataacg ccgcgatacc gacgccagca
      961 cgccgtaagt tgacgccaca cagttacagc ccgcttcgat cgccagttca acaatgtttt
     1021 tcgggtcaaa gtagagcggg ttagcagcaa atgaagctcc ggcagagtgc tcaacgccct
     1081 ggtcaaccgg cagaatagaa agatatcctg tgccagccag acgcccggtg ttgtacaacg
     1141 tctgcatatt acgtaacacc gctggcgggc gattattgtc aatcattacg cggtctacgt
     1201 agtcatgtcc ggggagataa agctggtcag aaggaattgt catacaacgg tgctgtaaaa
     1261 ggttgtcggc gtctttgcca agcaactgcg caatatctgt cattactatg ctcccgtaaa
     1321 ttccgattgg atatcggcta tggattgtcc tggcccgcct tttgcgggca atcataatcc
     1381 tggtcgttac gggcgagttt ttccatcttt tggatgtttt ttcagcgttt tctgttggct
     1441 cgattcatca gaaaaaatgt tagcgcggtc aagttttcac cgcaaaggta tttaaaaggt
     1501 attattaagt ggtattgtca tcgcgtacct tacattacct gtcatgaagg aattaaaaga
     1561 tgaaaacaac agcaaagctg tcgttcatga tgtttgttga atggtttatc tggggcgcgt
     1621 ggtttgtgcc attgtggttg tggttaagta aaagcggttt tagtgccgga gaaattggct
     1681 ggtcgtatgc ctgcacagcc attgcggcga tcctgtcgcc aattctggtt ggctccatca
     1741 ctgaccgctt tttctcggcg caaaaagtgc tggcggtatt gatgttcgca ggcgcgctgc
     1801 tgatgtattt cgctgcgcaa cagaccactt ttgccgggtt cttcccgtta ctgctggcct
     1861 actcgctaac ctatatgccg accattgcgc tgactaacag tatcgctttt gccaacgtgc
     1921 cggatgtgga gcgtgatttc ccgcgcattc gtgtgatggg cactatcggc tggattgcct
     1981 ctggtctggc atgtggtttc ttgccgcaaa tgctggggta tgccgatatc tcaccgacta
     2041 acatcccgct gctgattacc gccggaagtt ctgctctgct cggtgtgttt gcgtttttcc
     2101 tgcccgacac gccgccaaaa agcaccggca aaatggacat taaagtcatg ctcggcctgg
     2161 atgcgctgat cctgctgcgc gataaaaact tcctcgtctt tttcttctgt tcattcctgt
     2221 ttgcgatgcc actggcgttc tattacatct ttgccaacgg ttatctgacc gaagttggca
     2281 tgaaaaacgc caccggctgg atgacgctcg gccagttctc tgaaatcttc tttatgctgg
     2341 cattgccgtt tttcactaaa cgctttggta tcaaaaaggt attattgctt ggtctggtca
     2401 ccgctgcgat ccgctatggc ttctttattt acggtagtgc ggatgaatat ttcacctacg
     2461 cgttactgtt cctcggtatt ttgcttcacg gcgtaagtta cgatttttac tacgttaccg
     2521 cttacatcta tgtcgataaa aaagcccccg tgcatatgcg taccgccgcg caggggctga
     2581 tcacgctctg ctgccagggc ttcggcagtt tgctcggcta tcgtcttggc ggtgtgatga
     2641 tggaaaagat gttcgcttat ccggaaccgg taaacgggct gactttcaac tggtccggga
     2701 tgtggacttt cggcgcggtg atgattgcca ttatcgccgt gctgttcatg atttttttcc
     2761 gcgaatccga caacgaaatt acggctatca aggtcgatga tcgcgatatt gcgttgacac
     2821 aaggggaagt taaatgaaaa cagaacgtat tctcggtgct ctttatgggc aggcgttagg
     2881 ggatgcgatg gggatgccct ccgagctttg gccacgcagc cgcgttaaag cacactttgg
     2941 ctggattgac cgttttctgc ctggaccaaa ggagaataac gcggcctgtt attttaaccg
     3001 cgccgaattc accgacgata cctcgatggc gctgtgtctg gcggatgcgt tactggaacg
     3061 tgaaggcaag atcgatccgg atctgattgg gcgtaatatt ctcgactggg cgctgcgttt
     3121 cgacgccttt aacaaaaacg tactaggtcc gacctcgaag attgcgctta acgccattcg
     3181 cgacggtaaa cccgttgctg aactggaaaa caacggcgtg accaacggcg cagcgatgcg
     3241 cgtctcgcca ttaggttgtt tgcttccggc gcgtgatgtt gattccttta ttgatgatgt
     3301 ggcgctggcg tcgagcccga cccataaatc cgatctggca gttgcaggtg cggtagtcat
     3361 cgcatgggcg atttctcgtg ccattgacgg agaaagctgg tcagcgattg ttgattccct
     3421 gccttcaatt gcgcgacatg cacagcaaaa acgcatcact accttcagcg cctcactggc
     3481 agcacgtctg gagattgcgc tgaaaattgt gcgcaatgcc gacggcaccg aatccgccag
     3541 cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg ttccgtgcgc
     3601 cattgcgctg gttgaactgg cacaaaccga cccgaatcgc tgcgccgtcc tgtgcgctaa
     3661 ccttggcggc gacacagaca ccatcggcgc tatggcgacg gcgatttgcg gcgcgttgca
     3721 tggcgttaac gctatcgatc ctgcattaaa ggcggaactg gatgcggtaa atcagcttga
     3781 tttcaaccgc tatgccacag cgctggcgaa atatcgtcaa caacgggagg cggtatgagc
     3841 ggcgctcgat tacacacgct gctgcctgaa ttaacctcgc gtcagccggt gatggtcgtc
     3901 ggcgcggcgg tcattgatgt gatcgccgac gcttatgccc tcccctggcg tgggtgcgat
     3961 atcgaactga agcagcagag cgttaacgtt ggcggctgcg cactgaatat tgccgtggcg
     4021 ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc tcggtcaggg cgtgtgggcg
     4081 gagattattc gcaaccggat ggcgaaagag gggttaatca gcctgatcga taacgccgaa
     4141 ggcgataacg gctggtgtct ggcgctggtt gagccggatg gcgaacgcac ttttatgtca
     4201 ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg cgcgattaac cgttgcgcct
     4261 ggcagcctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga attgttagtg
     4321 gaatggctgg aaaagctgca agacgtgacg ccgtttatcg attttggccc acgtatcggc
     4381 gatatcccgg acgcattact ggcgcggatc atggcctgtc gacctttagt atcactcaat
     4441 cgtcaagagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat aacaacactt
     4501 ggcaagcaat ggcaggagaa atttgccgcg ccgttgatcg ttcgtctcga taaagagggt
     4561 gcatggtatt tcagcaacga cgcttctggc tgcattccgg catttccaac gcaagttgta
     4621 gacaccattg gggcgggcga cagtcatgca ggtggcgtac ttgccgggct ggcctctggt
     4681 ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgtgggt tgtcgggcat
     4741 cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacaa aaacgtatag
     4801 atcgctgcga cagtggctaa tgctgtactc aataggccgt tgctgttggt caagcgccac
     4861 ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact gaaactcggc
     4921 atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt gggtaaatgt gctggctgcg
     4981 gaaatagtca taaagtgaaa tcccgatggc atcaacatca tgaattaaat gcgccggaac
     5041 ccacgattcc tcaatcgata ccgcttcttc atcgacatag cgaatacgct taagcaaaaa
     5101 gacatcactt cctgcttcga ccgccagctg ctgcgcgact tcttcgggac atttcacgac
     5161 acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct gctgagaaaa
     5221 acctcgcgct tctttcaacg agtattcgaa gatgttgttg atctgcgtgc cgtaaccgcg
     5281 cgaacgcgtc accacaccct cttcttccag cgcctgcatc gccttgcgca ccgtaatgcg
     5341 cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc catgctccag
     5401 caccccgctg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa gcggcgtgtt
     5461 atccgccgct aaaatacgct cgttcagttg ggcaatcagc tgggtatgcg cttgttccat
     5521 ttgtcttttt cctgccgggg agtctggtgg gagtatatca ctgcttctta tttaaggcgt
     5581 ttctttaatc ccatcaacga atgcctgcaa ctcctccacc gtgccatgaa atacattaaa
     5641 atccaccgga ccattgatgc catctacctg tccacggtcg gaatgctgcc agaagcgcca
     5701 ggccatgccg tcattgtccg gacgacgttg ataatagtga gccacccacc acggatattc
     5761 attgaaatag cccgccagat tggtgtgata aaaaacggct cctgagtaaa taatcggctt
     5821 ttttcccgta cttttttcga ccatttttag ccactgactt actcgtttgc gtaattcttt
     5881 tgccgataat tttccccgtt cttctacgtc cagtaccgca gggaaatcgc cttgtgagaa
     5941 atccaccgtt tgcagaaata atcgtgcctg aaccggcgct gctaccgacg gggagaaata
     6001 atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac gcgaaaaata
     6061 cgggtccacc agcttttcgc cttccgtcgc cttaataaaa gcaaactgta agcggatacc
     6121 gttgtcgcgc atttttgcca cccgctgcca gtcgatccgc tcctgccagc gagaaacatc
     6181 tattccgtga atggtgtaac tggcaggaat gcgaatagcg aaagatttaa ccgggcgata
     6241 accgtagaaa tggatgtagt ctgtaatctg aactgctgtc gaatagaaaa aattcaccgt
     6301 ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata gagaggtgag
     6361 tttttttcga ctggtaattc ttagctgcat tggtttcatc cctgaatgtc agtgccaaag
     6421 gctgacaata accaaagcag ctatagtacg gtgctcacca ccaggcgtgg aagtggtgaa
     6481 ccggaccaat accgtgacca acttccagcg tgtcggcctg ggctaatgct gatgaaagcc
     6541 agctttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgt agtgcagcca
     6601 acgccgcaga gagcgtacaa ccagtgccgt gggtgttttt ggtcataatg cgcggtgcgg
     6661 taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg gctttgctca tcatccagat
     6721 gaccaccttt cattagcact gcgccacagc ccatcgccaa cagcgatcgc ccttgttcca
     6781 gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcggcagct tcgggcaagt
     6841 ttggcgttat taatgaaacc tgtggcaata atcgactgcg cagcgtagca accgccgaag
     6901 gtgaaagcag cgggtcgccg ctttttgcca gcataacggt gtcgagtacc acgttttgga
     6961 tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcggtttcc gccaacatac
     7021 cgattttagt ggtgtcgatt cgcacatcgc tgaacaccga atcgagctgg gcggcgacaa
     7081 aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc accagtgcag
     7141 taataactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg gcctgaatcc
     7201 ccgcaccacc actgggatca gtaccggcaa tcgtcagagc gttaattcgt ttcatgcctg
     7261 cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt ggaacaaaac tgcctggccc
     7321 ctcgcttctg gcgactgcgc gctctccggc ttgtttcatc cagtgacagg cagatgcgac
     7381 attttccagc gtatcgcctg gtaacgcaca gcaggcagcg acaaccgccg ataatgcaca
     7441 gccagttcct accactttgg tcattaacgg atcgccaccg tgaatgccaa cggcgcgatg
     7501 gccatcggta acataatcca tctcgccagt gaccacgacg attgcgccag tttcccgtgc
     7561 cagtgtttgt gcagcgggta tcgcgttagc tgcggcgtca gtggtatcca ctccccgtcc
     7621 accattagca atgccagcta atgccatgat ttccgaagca ttaccacgta tggcggttgg
     7681 cttatgagac agaagttcca gacaaaaacg gcggcgataa tcgagtgcac caaccgctac
     7741 tggatcaagc gtccagggtg tttgagagct ttttgcttgc tcaacggcgg cacacatcga
     7801 ctgtgcgcgt agctgcgtca gcgtgccaac gttaatcagc aacgcactgg caatagccgc
     7861 aaactgactg gcctcttcgg tttcgataac catcgctggc gatgcaccga gcgccagcaa
     7921 ggtattggca gtaaaggttt gcaccacatc gttggtcatg cagtgcacaa gaggggaatg
     7981 ttggtgaaaa aggtgtaacg cgtgcgcaga ttgcgctgaa ctcagcaggt cgacttgcat
     8041 agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac ttccctacgc
     8101 tggcattatc cagatcaggt gatacgggta tttctcagcc ttcacgcaga agggcacccc
     8161 gagtcgtttg gttgcgatga taaatcgcag aggaggatgg taatgtccag cgcacgcgtt
     8221 gtaaacgagg aaaaacggca gttttacaat cgcgttaata aattatttga tatatgaatc
     8281 cagcaccttc agaacgacat ccagatcttc ttcacgtttt agctcatccc cctggtgaac
     8341 gatgtgttcc gtcagatgac ctttaatcac ttcccgcatc agaccgttta ccgcgccacg
     8401 gatagcagca atctgttgta aaactgcagc gcattcgtgc ggttcgtcga gcattttctt
     8461 gagcgccacg acctggccct gaatcttact ggcacgcgct ttcagtttct gtttatcacg
     8521 gattgtatga gacatggcaa cacctggtta acaagaatat gaaaaatcat agcactatta
     8581 atctactggg gggtagtatc aggtactggg gggga
//
LOCUS       DAZFRJ010000178         8396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000178 DAZFRJ010000000
VERSION     DAZFRJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.180"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHKUN_003830"
     CDS             complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHKUN_003830"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO6662009.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHKUN_003831"
     CDS             complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHKUN_003831"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO6662010.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHKUN_003832"
     CDS             complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHKUN_003832"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO6662011.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4352..5467
                     /gene="malK"
                     /locus_tag="ACHKUN_003833"
     CDS             4352..5467
                     /gene="malK"
                     /locus_tag="ACHKUN_003833"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO6662012.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5539..6879
                     /gene="lamB"
                     /locus_tag="ACHKUN_003834"
     CDS             5539..6879
                     /gene="lamB"
                     /locus_tag="ACHKUN_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO6662013.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7122..8042
                     /gene="malM"
                     /locus_tag="ACHKUN_003835"
     CDS             7122..8042
                     /gene="malM"
                     /locus_tag="ACHKUN_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO6662014.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8271..>8396
                     /locus_tag="ACHKUN_003836"
     CDS             8271..>8396
                     /locus_tag="ACHKUN_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662015.1"
                     /translation="MISHILNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVPGLNR"
BASE COUNT         2075 a         2178 c         2176 g         1967 t
ORIGIN      
        1 aaagagaatt atcgtaagtg ggaaaacaaa taacgtaaaa ataataagct ctatgatggg
       61 aatattaacc ggcgaacgat tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc
      121 gccgaataac agtcacaagt tgggataacg taagttgagg gtgcagcggc ataacattgg
      181 cagaacaaca tctttaacct ttcacaccac ctgccgtcag gccgttgacc agccagcgtt
      241 gagccagcaa gaagacgatg gtgatcggta atgcagacat cacggcagcg gcggcaaagt
      301 caccccacag gtagttttgc gggttgaggt attgctgcat ccccacggcc agggtgtagc
      361 tgtttacgtc acgcagtaac agcgacgcga ccggaacttc agtaatggca gcgataaacg
      421 acaagatgaa taccaccgcc agaatcggta ctgacagcgg caacaggaca aggcggaagg
      481 cctgccacgg tgtcgcacca tccagcgcag ccgcttcttc cagcgaacta tcgatggttt
      541 cgaaatagcc tttgatggtc cagacatgca gcgcaatccc acccagatac gcgaaaatta
      601 cgccgccgtg agtattcagg ccaatgaatg gaatgtactc acccagacga tcaaacaacg
      661 catacaacgc gaccagtgaa agtactgccg ggaacatctg gaaaatcagc attcctttca
      721 gcagcgtcgc tttgcctgga aagcgcatac gggcgaaagc gtaggcgcag gtggtagaga
      781 gcgccacaat gccaatcgcg gaaatcccgg cgacctttac cgagttccac agccacagca
      841 gtaccgggaa tggcggtggc gtaatgcgac catcagcctg ttcaacgcta aaacctaacg
      901 ccagtttcca gtgatcccag gagatttgct ccgggatcag gctaccggtc gcgaagtttc
      961 cctgacgcag cgagatagcg acgaccatca gcagcgggaa cataatcgcc gcgataaaaa
     1021 gtagcagtag caggtgagta ataaacaaac gtgctttttg cgatttcggt tggaccattg
     1081 ccatttttgt tatctccctt aatcaaactt cattcgcgtg gctttcaggt tcactatcgc
     1141 cagcgcaccc accagcagga agatcagcgt ggcaattgct gctgccagac cgaagtcctg
     1201 acccccgccg ccttcaaaag cgatgcggta ggtgtagtta acaagcaggt cggtataacc
     1261 ggctggcgtg gtcgtgccaa gacgatccgg gccgccgttg gttaacagtt gaatcagcac
     1321 gaagttgtta aagttaaagg cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag
     1381 cagcggcagc gtaatcttaa agaagttctg gaacggacct gcgccatcca ttgctgaggc
     1441 ttcatacaaa tcgtccggaa tcgctttcag caagcccatg cagaggatca tcatgtacgg
     1501 ataacccagc caggtattga cgataatcag catcgtgcgg gcggtggtcg gatcgctgaa
     1561 ccaggcgggc ttcacgccaa acagcgcgct caacatcatg ttgatttcac cgaagctctg
     1621 gttaaacaac cctttgaaaa tcaagattga aatgaacgat ggcaccgcgt agggcagaat
     1681 cagcaggacg cgatagaccg ctttgccgcg caacgcttcc cactgcacca gacacgccag
     1741 aaccatgccg accgccaccg ttaaaaagac agtgatcagc gagaacacca cggtccagac
     1801 gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggtg aagacgcggg taaagttttt
     1861 ccagccagta gtcacggtgt aaccggggct tagcttttca tcgccccagt tgccgtcggc
     1921 ggtaatggac tggtaaaagc caatttggtt attcggacga tatttcacgc cgctctgatt
     1981 attcgtcaac gtgccgtcac cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg
     2041 cagggagctc atcatcactt tgttgccatc cggcagaatg gcggtaatgt cactcagcgc
     2101 ctgacgattc tgggtaatca cgcgcagatt cgcgcgttcg ccttcgggct gggtggtcgt
     2161 ttctttcagt tgcagttttt gctcgccgcc aaatttaaaa gcgtcggaga ggtaattttt
     2221 gccggtttcg ccgtcgctga gggctagttg ccactcatcg cccgccgggt aaagaccaaa
     2281 attataggtt ttgcctgctt gccaggagcg atctagcaac acttcctgcg cacgttcaaa
     2341 agtcagctgg ttagtgctgc tgtagttggt gaaggcaatg gcgatggtgc agaccagagg
     2401 gaagaggacg aataatccca ttccagccat tcccgggtaa acatagcgcc aggcgtaggc
     2461 tttacgattg gcgaaaatat acagccccgc tgaactcaat atcagcgtgg taatggcgaa
     2521 caggtattcc ccttgtgcgt acattaaaac aacaaggtaa cccaccagca ggccgagcag
     2581 acctaacact gaccatttca gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc
     2641 catggggttc ttcctcattc caggacggat aaggctttca cgccttatcc gacaacaact
     2701 gcctgatgcg acgctgacgc gtcttatcag gcctacatac gtttcggttt tgtaggccgg
     2761 acaaggcgtt cacgccgcat ccggcatttc acagcattac ttggtgatac gagtctgcgc
     2821 gtctttcagg gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg
     2881 cacggcatac cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc
     2941 gttttccatg gtggctgcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag
     3001 cgctacggca cccagcggtt tgtctttatt aaccgcttcc agaccttcgt cagtcagcag
     3061 atagttttcg aggaactctt tcgccagctc tttgttcgga ctggcggcgt taataccggc
     3121 gctcagcacg ccaacgaacg gtttagatgg ttgacccttg aaggtcggca gtaccgttac
     3181 accataattc actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat
     3241 cgctgtttcg cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat
     3301 gtgtttgttt ttaatcaggt caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc
     3361 cacgcccacg tctttaatgt cgtatttgcc attttcatac ttgaacgcat aacccccgtc
     3421 agcagcaatc agcggccagg tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt
     3481 acctttcgct ttcagttctt tatccagcgc cgggatctct tcccaggttt ttggtgggtt
     3541 cggcagcaga tctttgttat aaatcagcga taacgcttca acagcgatcg ggtaagcaat
     3601 cagcttgccg ttgtaacgta cggcatccca ggtaaacgga tacagcttgt cctggaacgc
     3661 tttgtccggg gtgatttcag ccaacaggcc agattgagcg tagccaccaa aacggtcgtg
     3721 cgcccagaag ataatgtcag ggccatcgcc agttgccgca acctgaggga atttctcttc
     3781 cagtttatcc ggatgctcaa cggtgacttt aattccggta tctttctcga atttcttacc
     3841 gacttcagcg agaccgttat agcctttatc gccgttaatc cagattacca gtttaccttc
     3901 ttcgattttg gcgagagccg aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag
     3961 gatgcgtgca cctgttttta ttttcataat ctatggtcct tgttggtgaa gtgctcgtga
     4021 aaacacctaa acggactcta gtttctttat acggcaacct ctttccatcc tccttgcccc
     4081 tacgccccac cgttgctttg tgtgatctct gttacagaat tggcggtaat gtggagatgc
     4141 gcacataaaa tcgccacgat ttttgcaagc aacatcacga aattccttac atgacctcgg
     4201 tttagttcac agaagccgtg ttctcatcct cccgcctcct cccccataaa aaagccaggg
     4261 ggtggaggat ttaagccatc tcctgatgac gcatagtcag cccatcatga atgttgctgt
     4321 cgatgacagg ttgttacaaa gggagaaggg catggcgagc gtacagctgc aaaatgtaac
     4381 gaaagcctgg ggcgaggtcg tggtatcgaa agatatcaat ctcgatatcc atgaaggtga
     4441 attcgtggtg tttgtcggac cgtctggctg cggtaaatcg actttactgc gcatgattgc
     4501 cgggcttgag acgatcacca gcggcgacct gttcatcggt gagaaacgga tgaatgacac
     4561 tccgccagca gaacgcggcg ttggtatggt gtttcagtct tacgcgctct atccccacct
     4621 gtcagtagca gaaaacatgt catttggcct gaaactggct ggcgcaaaaa aagaggtgat
     4681 taaccaacgc gttaaccagg tggcggaagt gctgcaactg gcgcatttgc tggatcgcaa
     4741 accgaaagcg ctctctggcg gtcagcgtca gcgtgtggcg attggccgta cgctggtggc
     4801 cgagccaagc gtatttttgc tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt
     4861 gcaaatgcgt atcgaaatct cccgtctgca taaacgcctg ggccgcacaa tgatttacgt
     4921 cacccacgat caggtcgaag cgatgacgct ggccgacaaa atcgtggtgc tggacgcagg
     4981 tcgcgtggcg caggttggga aaccgctgga gctgtaccac tatccggcag accgttttgt
     5041 cgccggattt atcggttcgc caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc
     5101 aatcgatcaa gtgcaggtgg agctgccgat gccaaatcgt cagcaagtct ggctgccagt
     5161 tgaaagccgt gatgtccagg ttggagccaa tatgtcgctg ggtattcgcc cggaacatct
     5221 actgccgagt gatatcgctg acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact
     5281 cggcaacgaa acccaaatcc atatccagat cccttccatt cgtcaaaacc tggtgtaccg
     5341 ccagaacgac gtggtgttgg tagaagaagg tgccacattc gctatcggcc tgccgccaga
     5401 gcgttgccat ctgttccgtg aggatggcac tgcatgtcgt cgactgcata aggagccggg
     5461 cgtttaagca ccccacaaaa cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc
     5521 aatgactcag gagatagaat gatgattact ctgcgcaaac ttcctctggc ggttgccgtc
     5581 gcagcgggcg taatgtctgc tcaggcaatg gctgttgatt tccacggcta tgcacgttcc
     5641 ggtattggct ggacaggtag cggcggtgaa caacagtgtt tccagactac cggtgctcaa
     5701 agtaaatacc gtcttggcaa cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa
     5761 gtgtggaaag agggcgataa gagcttctat ttcgacacta acgtggccta ttccgtcgcg
     5821 caacagaatg actgggaagc taccgatccg gccttccgtg aagcaaacgt gcagggtaaa
     5881 aacctgatcg aatggctgcc aggttccacc atctgggcag gtaagcgctt ctaccaacgt
     5941 catgacgttc atatgatcga cttctactac tgggatattt ctggtcctgg tgccggtctg
     6001 gaaaacatcg atgttggctt cggtaaactc tctctggcag caacccgctc ctctgaagct
     6061 ggtggttctt cctcttttgc cagcaacaat atttatgact ataccaacga aaccgcgaac
     6121 gacgttttcg atgtgcgttt agcgcagatg gaaatcaacc cgggcggcac attagaactg
     6181 ggtgtcgact acggtcgtgc caacctgcgt gataactatc gtctggttga tggcgcatcg
     6241 aaagacggct ggttattcac tgctgaacat actcagagtg tcctgaaggg ctttaacaag
     6301 tttgttgttc agtacgctac tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt
     6361 tctggcgtcg cgtttgataa cgaaaaattt gcctacaata tcaacaacaa cggtcacatg
     6421 ctgcgtatcc tcgaccacgg tgcgatctcc atgggcgaca actgggacat gatgtacgtg
     6481 ggtatgtacc aggatatcaa ctgggataac gacaacggca ccaagtggtg gaccgttggt
     6541 attcgcccga tgtacaagtg gacgccaatc atgagcaccg tgatggaaat cggctacgac
     6601 aacgtcgaat cccagcgcac cggcgacaag aacaatcagt acaaaattac cctcgcacaa
     6661 caatggcagg ctggcgacag catctggtca cgcccggcta ttcgtgtctt cgcaacctac
     6721 gccaagtggg atgagaaatg gggttatgac tacaacggcg atagcaaggt taacccgaac
     6781 tacggcaaag ccgttcctgc tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac
     6841 gagtggacct tcggtgccca gatggaaatc tggtggtaat agcaaaacct gggccggata
     6901 aggcgtttac gccgcattcg gcaaccaacg cctgatgcga cgcttgcgcg tcttatcagg
     6961 ccgacaacgg ctgtcaaatg taggccggat aaggcgttta cgccgcatcc ggcataaaaa
     7021 caggttgtca ttatctgaaa ggggcgaaag cccctctgat tatcgggttt agcgcgctat
     7081 tgcctggcta ccgctgagct ccagattttg aggtgaaaac aatgaaaatg aataaaagtc
     7141 tcatcgccct ctgtttatca gcagggttac tggcaagcgc gcctggaatc agccttgccg
     7201 atgttaacta cgtaccgcaa aacaccagcg acgcgccagc cattccatct gctgcgctgc
     7261 aacaactcac ctggacaccg gtcgatcaat ctaaaaccca gaccacccaa ctggcgaccg
     7321 gcggccaaca actgaacgtt cccggcatca gtggtccggt tgctgcgtac agcgtcccgg
     7381 caaacattgg cgaactgacc ctgacgctga ccagcgaagt gaacaaacaa accagcgttt
     7441 ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc atcagccttc ttccccagca
     7501 gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga tcggctggaa ggcgttatgc
     7561 gcctgacacc ggcgttgggg cagcaaaaac tttatgttct ggtctttacc acggaaaaag
     7621 atctccagca gacgacccaa ctgctcgacc cggctaaagc ctatgccaag ggcgtcggta
     7681 actcgatccc ggatatcccc gatccggttg ctcgtcatac caccgatggc ttactgaaac
     7741 tgaaagtgaa aacgaactcc agctccagcg tgttggtagg acctttattt ggttcttccg
     7801 ctccagctcc ggttacggta ggtaacacgg cggcaccagc tgtggctgca cccgctccgg
     7861 caccggtgaa gaaaagcgag ccgatgctca acgacacgga aagttatttt aataccgcga
     7921 tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt aaaactgctt gatgaagctg
     7981 aacgcctggg atcgacatct gcccgttcca cctttatcag cagtgtaaaa ggcaaggggt
     8041 aattacgccc cacagtgctg attttgcaac aactggtgcg tctcctggcg cacctttttt
     8101 tatgcttcct tcctgggata tgagcgattt tttatagtaa ctcacttctt cttcactaag
     8161 aatatccatt atctcaatgc cttatcagag attcttttcc tttcgccggt agtgtctgga
     8221 cattcaggct acttttccag gttattttat ttctgttatg cagaggtttt atgataagtc
     8281 atatcctaaa ttctggcggc aataactctt tgatgaaaca tgatgtggtg caaggaaata
     8341 atatagtaga tcttgattta ctacgtaatt tcaatggggt gccaggttta aacaga
//
LOCUS       DAZFRJ010000179         8365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000179 DAZFRJ010000000
VERSION     DAZFRJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.181"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(95..301)
                     /locus_tag="ACHKUN_003837"
     CDS             complement(95..301)
                     /locus_tag="ACHKUN_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO6662016.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            614..703
                     /gene="kdpF"
                     /locus_tag="ACHKUN_003838"
     CDS             614..703
                     /gene="kdpF"
                     /locus_tag="ACHKUN_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO6662017.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            703..2376
                     /gene="kdpA"
                     /locus_tag="ACHKUN_003839"
     CDS             703..2376
                     /gene="kdpA"
                     /locus_tag="ACHKUN_003839"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO6662018.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2399..4447
                     /gene="kdpB"
                     /locus_tag="ACHKUN_003840"
     CDS             2399..4447
                     /gene="kdpB"
                     /locus_tag="ACHKUN_003840"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO6662019.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4456..5028
                     /gene="kdpC"
                     /locus_tag="ACHKUN_003841"
     CDS             4456..5028
                     /gene="kdpC"
                     /locus_tag="ACHKUN_003841"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO6662020.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5021..7705
                     /gene="kdpD"
                     /locus_tag="ACHKUN_003842"
     CDS             5021..7705
                     /gene="kdpD"
                     /locus_tag="ACHKUN_003842"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO6662021.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7702..>8365
                     /gene="kdpE"
                     /locus_tag="ACHKUN_003843"
     CDS             7702..>8365
                     /gene="kdpE"
                     /locus_tag="ACHKUN_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO6662022.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGY"
BASE COUNT         1740 a         2176 c         2441 g         2008 t
ORIGIN      
        1 taacgatgtc taaatattcc tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc
       61 gctggcggtt ttcaatgtat tcaaaactca gcaatcaata aaaatcacca gttgcctttt
      121 cggcctgatc catccacacc ggtttatcgc tggttttcga ccagacgcga tgcaggtagc
      181 tgtaaaagcg ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg
      241 caaccgcata agtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca
      301 tacttttctc ccccttttgt atctacccgg tgaatggtac cggaaaaatg aatttgttta
      361 tctgatgaaa atagtaccgc cttttgtgta attttactac tcatccgacc acttattttt
      421 gcttattgat ggtttattta cattcatcct gtaattaagt tacacaaaag ttaaattaat
      481 actaaacatt agttaaatca tggcttttgc catttttata ctttttttac accccgcccg
      541 cagatttttg cgaaatcttt gcagccagaa ttctaccctt ccggtatcac ttttaggcca
      601 ctggaggtgc actgtgagtg caggcgtgat aaccggcgta ttgctggtgt ttttattact
      661 gggttatctg gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc
      721 ttactgatcg ccacgttttt actggtgtta atggtgctgg cgcgtccttt aggcagcggg
      781 ctggcgcggc tgattaatga cattcctctt ccaggtacag cgggcgttga acgtatactc
      841 tttcgtttac ctggcgtctc tgaccatgag atgaactgga agcaatatct ttgtgccatt
      901 ctcggcctga acatgctggg gctggcggtg ctgtttttta tgttgctcga tcagcactat
      961 ctgccgctta atccacagca gttgccgggg ctgtcgtggg atctggcgct gaatgccgcc
     1021 gtcagctttg tcacgaatac caactggcaa tcttacagcg gtgaaaccac gttgagctat
     1081 ttcagccaga tggctggctt aacggtgcaa aactttcttt ctgccgccag cgggattgcg
     1141 gtgatttttg ccctcatccg tgcgtttacc cgccagagca tgagcacact cgggaatgcc
     1201 tgggtcgatc tgctacgcat cacgttatgg gtgctcgtcc ctgtggcgtt gttgatcgca
     1261 ctgtttttta ttcaacaagg tgcgctgcaa aactttcagc cttatcaggc tgtgaatacc
     1321 gttgaaggag cgcaacagct gttacccatg gggcctgtag cttctcagga agcgatcaag
     1381 atgctcggta ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac
     1441 ccaaccgcac tgaccaactt cgtgcagatg ctggcgatct tcttgatccc aacggcgctg
     1501 tgctttgcct ttggtgaagt gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg
     1561 atgtcagtga tttttgtcat ctgcgtaggc gtggtgatgt gggcagaagt tcagggtaat
     1621 cctcatctgc tggcgctcgg cgcagacagc agcatcaata tggaaggtaa agagagccgt
     1681 ttcggcgtac tggtcagtag cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg
     1741 gtgattgcga tgcatgattc gtttaccgct ctcggtggca tggtgccgat gtggctgatg
     1801 caaattggtg aagtggtgtt cggcagtgtc ggttctggtc tttacggcat gatgctgttt
     1861 gtcctgctgg cggtgtttat tgccgggctg atgattggtc gtacaccgga atatctgggt
     1921 aaaaaaatcg acgtacgcga gatgaaactg actgcactgg cgattctggt taccccgacg
     1981 ctggtgctga tgggcgcggc gctggcaatg atgaccgacg ccggacgtag cgccatgctc
     2041 aaccctggcc cgcatggttt tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac
     2101 aacggcagcg cctttgccgg attaagcgcc aactccccgt tctggaactg tttactggcg
     2161 ttctgcatgt ttgtcggtcg cttcggggtg attatcccgg tgatggcaat tgccggttcg
     2221 ctggtgagta aaaagagcca acccgccagc tccggcacgc tgccaacgca cggcccgctg
     2281 tttgttggcc tgttaatcgg caccgtgttg ctggttggcg cactgacctt tatccctgcc
     2341 ctggcgcttg gtccggtggc ggaatatctc tcctgatgat attgagtgag cactgaatat
     2401 gagtcgtaaa caactggcgc tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc
     2461 ggtgaaaaaa ttaaacccgc aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat
     2521 cggcagtctg ctgaccacct gtattagcat cgcgatggca agcggtgcga tgcctggcaa
     2581 tgcgctgttt agcgcggcaa ttagcggctg gctgtgggtc accgtactgt tcgctaattt
     2641 cgccgaggcg ctggcagaag gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa
     2701 aaaaactgcc tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt
     2761 tcctgccgat caactgcgta aaggtgacat cgtgctggta gaagctggcg atattatccc
     2821 ctgcgatggt gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga
     2881 atcggcaccg gtgatccgtg aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg
     2941 tattctttct gactggctgg tgattgagtg tagcgttaac cccggtgaga catttctgga
     3001 tcggatgatc gcgatggtgg aaggcgcaca gcgacgcaaa acaccgaacg agattgccct
     3061 gactatcctg ctgattgctc tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc
     3121 gttttccgcg aggggcggta atgccgtgag tgtaacggta ctggtggcgc tgctggtctg
     3181 tctgatccca accactattg gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg
     3241 gatgctgggc gcgaatgtga ttgccaccag cggacgtgca gttgaagcgg caggtgacgt
     3301 tgacgttctg ctactggata aaaccggcac catcacactc ggtaaccgtc aggcgtcgga
     3361 gtttatccct gcgcagggcg tggatgaaaa aacgctggct gacgccgcac aactggcttc
     3421 gctggctgat gaaacgccgg aaggccgcag tattgtgatc ctcgccaagc aacgttttaa
     3481 cctgcgcgag cgcgatgtgc agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag
     3541 ccggatgagc gggatcaaca tcgacaaccg catgatccgt aaaggttctg tcgatgccat
     3601 tcgccgccat gttgaggcta acggcggtca cttccctacc gatgttgatc aaaaagtcga
     3661 tcaggttgcg cgtcaggggg ccacgccgct ggtggtggtg gaaggttccc gtgtgctggg
     3721 cgttattgcg ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg
     3781 caaaatgggc attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgccat
     3841 tgctgcggaa gcgggcgtcg atgattttct cgccgaagcg acaccggagg ccaagctggc
     3901 attgattcgt cagtatcagg cggaaggccg tttggtggcg atgaccggcg acggcaccaa
     3961 cgatgctcca gcgctggcgc aggcagatgt cgcggtggcg atgaactccg gtactcaggc
     4021 ggcgaaagaa gcgggcaata tggtcgatct cgactctaac ccgaccaagt tgatcgaggt
     4081 ggtacatatt ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc
     4141 caacgatgtg gcgaaatact tcgccattat tccggcggcg ttcgcggcaa cgtatccgca
     4201 gttaaatgcg ctgaacatca tgcgccttca ttcgcccgac tccgccattc tcagtgcggt
     4261 cattttcaac gccttgatta tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta
     4321 taaaccgctt accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg
     4381 gctgctggtg ccgtttattg gtatcaaagt cattgattta ctgctgaccg tttgcggtct
     4441 ggtgtgaggt ttaccatgcg tggcttacgt ccggcattat cgacatttat ctttctgtta
     4501 ttgattactg gcggcgttta cccgctgctg accaccgtgc tggggcaatg gtggtttccc
     4561 tggcaggcca acggttcgtt gattcgtgaa ggtgatacgg tgcggggttc ggcattaatc
     4621 gggcagaatt ttaccggcaa cggctatttt cagggtcgcc cgtcggcaac ggcagaaatg
     4681 ccctataatc cacaggcttc tggcgggagc aatctggcgg tcagtaaccc tgagctggat
     4741 aaacaaatag ccgcacgcgt tgctgcatta cgggccgcta acccggatgc cagtacgaac
     4801 gttccggttg aactggtgac ggcatcggca agcgggctgg acaataatat caccccgcaa
     4861 gcggcggtct ggcaaatccc acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc
     4921 acgcaactga tcgcaaaata cagccaacaa ccgctggtga aatatatcgg ccagccggtc
     4981 gtcaacattg ttgaactcaa tctggcgctg gataaacttg atgaataacg aacccttacg
     5041 tcccgacccc gatcgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt
     5101 tttcttcggt gcctgtgcag gcgtcgggaa gacctgggcg atgctggcag aagcccagcg
     5161 actgcgggcg caagggctgg atattgtggt tggcgtggta gaaacccatg ggcggaaaga
     5221 taccgccgcc atgcttgaag ggctggctgt tctgccgccc aaacgccagg cgtaccgtgg
     5281 gcggcatatc agcgagtttg atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt
     5341 aatggacgaa ctggcgcaca gtaatgcgcc aggttctcgt catcctaaac gctggcagga
     5401 tatcgaagaa ctgctggaag ctggcattga tgttttcaca accgtcaacg ttcagcatct
     5461 ggaaagtctg aatgatgtgg tcagcggcgt caccggaatt caggtacggg aaaccgtgcc
     5521 cgatcctttt ttcgatgccg ccgacgacgt agtgctggtg gacttgcccc cggacgatct
     5581 gcgccagcgg ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga
     5641 acattttttc cgcaaaggca atctgatcgc cctgcgtgaa ctggcactgc gccgtactgc
     5701 cgatcgcgta gatgagcaaa tgcgcgcctg gcgggggcat cctggtgaag agaaagtgtg
     5761 gcacacgcgc gacgcgatcc ttttatgcat cgggcataac accggcagtg agaaactggt
     5821 ccgcgcggcg gcgcggttgg cctcgcgcct gggtagcgtc tggcacgcgg tgtatgttga
     5881 aacccctgcc ctgcatcgct taccggaaaa aaaacgtcgg gcaattctca gcgccttacg
     5941 tctggcgcag gagctgggcg cggagacggc tacacttact gatccagcgg aagaaaaagc
     6001 agtagtgcgt tatgcccgtg aacataatct cggcaagatt attctcggtc gcccggcctc
     6061 gtgccgctgg tggcgtcggg aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct
     6121 cgatcaggtg ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga
     6181 tagccgctct tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc
     6241 gttatgcgcc gttatcactt taattgccat gcagtggctg atggcgtttg atgccgccaa
     6301 cctggtgatg ctgtatctgc ttggcgtggt ggtggtggcg ctattttatg gacgctggcc
     6361 ttcggtggtt gctaccgtca ttaatgtggt gagtttcgat ctctttttta tcgccccacg
     6421 cggcacgctc gctgtctctg atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt
     6481 cgggctggtg atcgggaacc ttactgcggg tgtgcgttat caggcgcggg tagcccgtta
     6541 ccgcgagcaa cgcacacggc acttatatga aatgtcgaaa gctctggcgg tgggccgcag
     6601 tccgcaggac atcgccgcca ccagcgaaca atttattgcc tccacgtttc atgcccgcag
     6661 tcaggtgttg ttgcccgatg acaacggtaa attgcagccg ttaacacatc cgcaaggaat
     6721 gacgccatgg gacgatgcca tcgcgcagtg gagttttgat aaaggcctgc ctgcgggtgc
     6781 gggcaccgac acgttacccg gcgtgccgta ccagattttg ccgctaaaaa gcggcgagaa
     6841 aacctacgga ctggtggtgg tggaaccggg gaatctgcgt cagttgatga tcccggagca
     6901 gcagcgcctg ctagagacgt ttacgctgtt agtcgccaat gcgtttgagc ggctgacgct
     6961 gaccgccagt gaagaacagg cgcggatggc aagcgaacgt gaacagatcc gcaacgccct
     7021 gctggcggcg ctttcgcatg atttacgcac gccgcttacg gtgctgtttg gtcaggcaga
     7081 aatcttaacg ctcgatctgg caagcgaagg atcgccccac gcccgccagg ccagcgagat
     7141 tcgtcagcat gtgctgaaca ctacccgact ggtgaataat ctactggata tggcgcgaat
     7201 tcagtccggc ggctttaatt tgaagaaaga gtggttaacg ctggaagaag tagtcggcag
     7261 cgctctgcaa atgctggaac cgggtttatc gtcgcccatc aatctttctc tgccagaacc
     7321 gctgacctta atccacgttg acgggccact ctttgaacgg gtgctgatta atctgctgga
     7381 gaacgcggtg aaatatgcgg gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg
     7441 cgaaaatcta caactggatg tctgggataa cggccccggt cttccgccag gccaggagca
     7501 gacgatattt gataagtttg ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct
     7561 tggactggca atttgtcggg cgatagtgga tgtacacggg ggcactatta ccgcgttcaa
     7621 ccgaccggaa ggtggtgcct gttttcgtgt tacacttccc cagcaaaccg ctcctgaact
     7681 tgaagacttt catgaggata tgtgacaaac gttctgattg ttgaagatga acaggctatt
     7741 cgtcgctttc tgcgcacggc gctggagggc gacgggatgc gcgtctatga ggccgaaacg
     7801 ctgcaacgcg gcttgctgga agcggctacc cgtaagccag atttgattat tctcgatctc
     7861 ggcctgcccg atggtgatgg gattgagttt atccgcgacc tgcgccagtg gagcgcggtg
     7921 ccggtgattg tgctttccgc acgcagcgaa gagagcgaca aaatcgccgc gctggatgcc
     7981 ggagcggatg attatctgag taagccgttt ggcattggcg aattgcaggc ccgtctgcgc
     8041 gtcgctttac gccgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccgat
     8101 gttaccgtcg atttagccgc ccgcgtgatt caccggggtg aggaagaggt gcatctcacg
     8161 ccgattgagt tccgcctgct ggcggtgctg ctaaataatg ccgggaaagt gctcacccag
     8221 cgccagttac tcaatcaggt gtgggggcca aacgcggtcg aacacagtca ctatttgcgt
     8281 atttatatgg gacatctgcg acaaaaactg gaacaggatc ccgctcgccc ccgccatttc
     8341 attactgaaa ccggtattgg gtatc
//
LOCUS       DAZFRJ010000180         8229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000180 DAZFRJ010000000
VERSION     DAZFRJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.182"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..183)
                     /locus_tag="ACHKUN_003844"
                     /pseudo
     CDS             complement(1..183)
                     /locus_tag="ACHKUN_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            333..1001
                     /locus_tag="ACHKUN_003845"
     CDS             333..1001
                     /locus_tag="ACHKUN_003845"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6662023.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(946..1083)
                     /locus_tag="ACHKUN_003846"
     CDS             complement(946..1083)
                     /locus_tag="ACHKUN_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO6662024.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1056..1640)
                     /locus_tag="ACHKUN_003847"
     CDS             complement(1056..1640)
                     /locus_tag="ACHKUN_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6662025.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1640..4600)
                     /locus_tag="ACHKUN_003848"
     CDS             complement(1640..4600)
                     /locus_tag="ACHKUN_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662026.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAETPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTSSAGAHSHVDGRRFNTSTFKDTYQYGSTAVGSNSYQVQGAVGLGI
                     GTMANTNSAGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
     gene            complement(4665..5264)
                     /locus_tag="ACHKUN_003849"
     CDS             complement(4665..5264)
                     /locus_tag="ACHKUN_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6662027.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(5335..>8229)
                     /locus_tag="ACHKUN_003850"
     CDS             complement(5335..>8229)
                     /locus_tag="ACHKUN_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6662028.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFT
                     QLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGK
                     SAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDA
                     NDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNET
                     PMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGRVNANAG
                     TLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDD
                     HNFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPG
                     SYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT         1761 a         2323 c         2023 g         2122 t
ORIGIN      
        1 aatgactgtt actgtaacat gagttacaaa cagcttcata cccagagcta ctgatgtatc
       61 aactcgattc aacaataccc gatatttatt ccatgcctcc agcaacgatc tttcttcctc
      121 cgttgcgatt tccagatcta cagcatcctg cagtggcgca atatactcac tgaattcctg
      181 gatgtagaac tgtgtggtga cggtcttcca gccattcggc tcctgttgta tcgaagcata
      241 ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca
      301 taattgattt aattcacaaa taaaactata acatggtgaa attaatgaaa aaaaacacag
      361 atgatggggc taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg
      421 gagtatcaaa tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc
      481 tggaacattt aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc
      541 acgtacctga gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg
      601 catctacaag ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac
      661 cttatcccgc ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact
      721 gcctgcctgg aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa
      781 ctgacgatgg aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct
      841 tcggtcaaaa actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt
      901 ccgaagaagg cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc
      961 aaggtactgt agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt
     1021 cttgcttaag acgtgctgcg gcataatccc aatgattact ccctgacagg gttcgtaggc
     1081 cactcaatat caggtgcagt tgatgtatca acacggttca gcaacacccg atactttttc
     1141 caggcttcca gcaatgaggt ttcttcctcc gttgcgattt ccagatctac agcatcctga
     1201 agtggcgcaa tatgctcact ggctacctgc atcaggctgt tttttatttc ttccgcctct
     1261 cggatccgga acagtttttc tgcttctgca tctttcaccc aggctgtgcc gttccacttc
     1321 tgaaactccc cttccggcga taaccaggta acattttccg gtaacggacc gagttcagaa
     1381 ataaataacg cgtcccctga cgctacgtca taaaccgttt taccccgatg gtcttcaacg
     1441 agatgccacg atgcctcatc actgttgaaa acagccacaa agccagcagg aatatctggt
     1501 ggtgcaatat cggtactgtt tgctggcaga cctgtatgag gcggaatata tgcatcacct
     1561 tcaccaataa attcattagt tccggccagc agattataaa tttttatggt ccgtgcttgt
     1621 tcactcattc tgaatgccat tatgcaagcc tcacaatata gttaaatgcg atgtttttga
     1681 cggtgttttc cgcgttacca gcagcgttaa cggtgatggt gtgtccatgt gagccaatcg
     1741 caacggagtg cgtatgagca ccaataccga cagtatgtgc gtgtgcacct gcgcttgcag
     1801 cagtgccgga cagtgagtgg gtatgcgcgc cagcagagtt cgtattcgcc atcgtgccaa
     1861 tgcccagacc aaccgcccct tgtacctggt atgagttact tccgacagct gtagaaccat
     1921 actgataggt gtctttaaaa gtgcttgtat taaacctgcg accatcaacg tgcgagtgtg
     1981 ctccggccga gcttgtagag ccgctcagac tgtgcgtatg agccccggtg ttattcgtgg
     2041 atttagtgcc gtaatcaaac gacgatgtgg ttttcgtccc caaatccgta ctggatgcgc
     2101 tggcgctgtg ggtgtgcgat ttaatgccgt cctgttcctg agacaatacg gcacgaccac
     2161 tggcgggttt gcccttaatc gtccagccac gcatatcagg gatcacgcct gacggataag
     2221 cggctgcaag tttcgggtat gcagatttgt caaaagtctg cccctgcatc agggcatagc
     2281 cagacggaac ggtatctgat ggccacggga ttggtgcgcc aggcggataa aactgctctg
     2341 atggcgtata gagtgaataa actgtaccgt ccgttaaccc ttccggctta ttagcagaat
     2401 atgctggtga cgtatgaatc gtcacgctgg cattactggt ataatcccat tgaatattta
     2461 caccagtcgc ataatttccg attgcaacgt aaatatcgta agtatcacca gatgtattga
     2521 cccaggcaaa atttgtaaac cctgtcgatg tgcgctgcca taaagcacca gtaatcccct
     2581 tcggattacc attacctgca cgcaaaacaa gttcagatat acctgcctgt tgaggtgacc
     2641 ccacgttaaa tcccgcgcca ccaatcaacg taattgaaac aacagaactc gcctgtggca
     2701 tggttaccgt tgctaatttg aaccaaccag caccaccgct gaatgacatt gttgttgagt
     2761 taagcgtacc aatatctttc ggcgtcagtg ttatatccgc tgaaagcgcc ttaccattca
     2821 ccttacgggc agaaggtacc cgaccattcg cattatcatt agctgctttc actgctttcg
     2881 gtgtctcggc aagcgtttca gatgcgctgt tggttgcact actgagctgg acaattcctt
     2941 tttgtgctgt cgtagcgtcc tgagcggtat atttcccgtt agcaaggtca taggctgcct
     3001 ttaccgactt tggcgttgcc gccagcattt cagatgtgct gttggtcgcg ctgctgagct
     3061 gaacgatccc tttctgtgct gtcgttgcat cctgtgcggt gtatttcccg ttagccaggt
     3121 catacgcggc cttaacggct tttggcgttg ccgccagtga ctcggaagtg ctgttagtcg
     3181 cactgctgag ctgtactatc ccctttttcg tcgtgctcgc atcctcaagc gccacggcgg
     3241 atgcaatatc ctctgcccgt ttagctgctg tctcggcgcg cgttgccgcg gattccgccg
     3301 tacttttgct ctgagctgcc gccgtcgcac tgccagctgc ctctgtcgcc ttcgtggatg
     3361 ccgtcgtggc gctgctcttc gctgctgacg cctgtctggt cgcctcatct tttgaagcag
     3421 acgccgatga tgccgatgac gccgccgaac tggctgacga tgcggcagcc gtttttgagg
     3481 attctgcgct ggtttccgac gctttcgcgt tcgtttcgga tgtcttcgct gcggaagcag
     3541 aactcgctgc tgcgctggcc tgttcagtgg cttcgccagc cttcgttgtg gctgttgaag
     3601 cggatgatgc ggcgctttct gccgattttc cggcggcggt ggcactggct gaggcctgcc
     3661 cggcacttgt tgacgcggca ctggcagacg acgcagccgc tgtttttgag cctgccgcag
     3721 ctgaggcgct ctgtcccgct gccgtctcag aagacttagc gttcgtctcg gacgtttttg
     3781 ccgccttcgc ggaatttcct gccgccgttg ccgaggaagc ggcactactg gcgcttgatg
     3841 atgcgttcgt ttctgatgat tttgccgctt cttttgaagc cgacgcatcc cgggctgagg
     3901 tggcagcttc tgacgctttc gtggtcgcgg tggatgcaga agtggctgct gattgttgtg
     3961 acgctgccgc attcgtttct gacgttttcg ccgcagcggc actggtggcc gccgcgcttt
     4021 ttgaggactc tgcagcggca gcactttttg atgcttcagt ggcctttgtt gatgccgttc
     4081 ctgcgctgga agacgctgac tgagccgacg acgcggcctg tccggctgac gtgctggctg
     4141 cgcgtgctga gcctgcagca tcagtcgcat gggttgccgc ctcacgggct gatgtgctgg
     4201 catcgctggc tgacttcttc gcggctgccg tgttctgtgc caccgcggac gcgttacgcg
     4261 ccacctcttc caccatcagt tcaaaacggc gcagtgcctc aggacgggca tcatcctccg
     4321 tcatggcacc gagaaaatca ttcagcgtac cgggtcggga atcttcatac acggtgatgg
     4381 tcccggcatg tgacggcggg aagccttcca ccaacagaat aacgctgtac tgaccgtact
     4441 caacgtccat gctgtaacgc ccggcttcat ccggattttc tgaggccagc gtgttcacca
     4501 ccaccgtggt actgttacgt tttgctttca gctggattgt gcagttctgt accggttttc
     4561 ctgtgccgtc tttcagtaca cctgaaatct ttactgccat attcacccca caaaaaagcc
     4621 cgcctgaacc ggcgggctgt cataacactg tgttacctgg ctaatcagaa tttataaccg
     4681 acacccacga tgaaaccgtc agtgcgccag tcgccactgc cggagccttc ataagcaata
     4741 tcaatggcca cggattcggt cgggttaaac tgcacgccag ctccccacgc cagagacgtg
     4801 ttgctgtggc gaccgtcatc acttccggtc agcacgtcgt gcgttttccc cttgttgtca
     4861 gttacgcgga gataatcccc ggagaaagtc gacacacggc tgtaagacac acccgccatc
     4921 gcatacgcgc tgaaccattc attcacgcgc acagacggcc ccgccatcac gctgaaccag
     4981 cggttacgca cggaatcttc atgccagcgg atatcgctgt aacgggtaag ctggcgattc
     5041 ttgtctcctg catagctgaa tgacgtcacc agccccagtg tgtccgtaaa ctcataacgg
     5101 tatttcacgt taatcccgtt cagatcatcg ctgccgggaa cgttcgtcga ggcatgaaga
     5161 taccccgcgc tcagcgtgga ctgatgttca gatgcccatg cagacgcacc ggatacggcc
     5221 agacaaatag ctgcggacaa aatggctgca taaagtttac gcataattac ctctcgcttt
     5281 tctgcaataa aaaaggcgtc attcctgacg ccctttattg gggttataaa tatttcaacg
     5341 aatactgata ccggaagccg cttttttggt cacaatcacc gtacagtcgg tgatattccc
     5401 tgccccctga ttgcctttct ggaaaatctt aaactccaga gtgacgctac caccaccact
     5461 aggcatatca ataactgcac tgtaactacc gggaatggcc cctttagttt ctctggatgc
     5521 gattaataca ccgtttttgc gaacttcaaa accataaccc gtgtatcttg tacctcccgg
     5581 gttattacca cttcccggat cgctatacgc tattccgtta aagataatgg gcggaataat
     5641 gatttgacgg tcaaagttat gatcatcgct gatggtgact gtaaccgtcc cgtttggtgt
     5701 ttccgtatta ccccacgtac cagcctgttt cgggaatgat ttggatacag ctttaacgaa
     5761 gtcacctctg acctgagtcg cctccagcat gcccttaatc gtacagtttt catttaccgt
     5821 gacattgttg agcgtcccgg cgttcgcatt cactctgcca ctgatatccg catttttagc
     5881 ggtcagcttt ccgtctgatg tcagggaaaa taccggagga ctgccaccgc tggtaatggt
     5941 gggggctgtc aggcgcttca ggaacacgtc gttcatgaat atctggttgc cctgcgccac
     6001 aaacatcggc gtttcattcc cgtttgccgg gtcaataaac gcgatacggt tagcggcaac
     6061 cagaaactgg ctcagcttgc cttcctccgt gtcctccatg ctgaggccaa tacccgcgac
     6121 ataatgtttg ccgtctttgg tctgctcaat tttgacgccc cacatggcat tccatttatc
     6181 gttagcgtcc ttccactctt tcgaaaactc atccagtctg ctggcgttat cctccgtcaa
     6241 atcaactttt tccagcagct ctttaccgag atgggattcg gttatcttgc ctttgaaaaa
     6301 atccaggtaa ccttccgcat catcgctcgc ccgaccgacg ccctccacga atgccgattt
     6361 gccaacggtg ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat
     6421 actggcggct atccagtaca gcgccgtacc aagatagcgg gctgtggttt caacctgcct
     6481 gatatcggta atccgcgttt ccgagaacca gaactcaaac tgtaccgtcg ggtcataaac
     6541 ggcaagatgc ggcgtggcgg ttatctgaaa atagcccggc gtcagctcaa tcctcgacgg
     6601 tgctgccggt gcggcaatcc ggaacgatac cgacgccgga tcgccctgct gcccccacgc
     6661 atttaccgcc cggactgtca gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt
     6721 ggtttccgtc gtccgggccg tgctgaccag ccgctcactg ccgtcatccg ctgttacggt
     6781 cagacggagc aggaagctca cgcccttcac caccttcggc gtgtcccagc gcgccagcac
     6841 ctgatattcc ccgctgtctg cggtgacttc ggcggtcagg tgctgcaccg ctggcggcgt
     6901 gacaccattc accgtgccgc tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc
     6961 ttctttttcc ggtacatgct gcacggcagt gatggcatac gtgccgtcat cgttatcacg
     7021 gatactcaca cagcggaaca ggcgctgacg caacgtcggc agcttcagcc cccacacact
     7081 gtattctgca acgccgtcag gaacccggtt cactttcacc ttaagtccgt cggtgacgga
     7141 ctgaacctcc acgctgaccg gattaccact tccgtcaacc aggcttatca gcgtggtacc
     7201 ggaggatggc agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc
     7261 cagcacgcga ccaccggtgc tgataccggc atagtcatca tcgaagattt caataacatc
     7321 gcccggtaca tggcgaagcc cttcagcacc cacgctgaaa tccacggtct gcgtctccag
     7381 cagttccgtt ttaatcagcc acagcccggc gcggtgtgcc tgcccccggc tggtacagcc
     7441 aaaggcatcc atcttcgtga cattacgacc gtaacgggca atggcctgcg tatcttcaac
     7501 aagctctgtc gccgtctccc agccgttgtt cgggtcaatc cagttcacct caacggcatt
     7561 atggcggtcc ttcagggcgc tgaagctgta gcggaacggc gcgccatcat ccggcatcac
     7621 cacattactg cggttatagg tccacacctt atctgatggt cggtcctgca cgaacgtcag
     7681 cgtctgcccg ttccataccg gcatacagcg catcgccgag cagaaatcac tgagcacatc
     7741 ccacgccttg cgctgtgtgg tcaggtatgc attacaggtg atgcgcggct ccgtgccgcc
     7801 aaagccgtcc ggtactgact ggtcgcagta ctggccgatg acatacagcg cccatttgtc
     7861 cacatccgcc gcaccaagac gtttccccat gccgtagcgt ggatgggtca gcatatccca
     7921 cagacaccag gccatgttgt tgctgtatgc cggtttaaac gttccgtccc agataccgct
     7981 gtattgtcgc gtctgcgggt tatagttcga cggcacctgc agaatgcgcc cgcgaagatg
     8041 ataattacga ctcacctgct ggctgccgaa ctgctccaag tccacctgta cgccgaccag
     8101 tgccgtgttc gggtagcact gtttcacatc gatgatttcg gtgtatgacg accagagcgt
     8161 tttgttctgc agctggtctg tggtgctgtc cggcgtcatc ctgcgcatcc ggatattaaa
     8221 cgggcgcgg
//
LOCUS       DAZFRJ010000181         8170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000181 DAZFRJ010000000
VERSION     DAZFRJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.183"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(383..1414)
                     /gene="rsmC"
                     /locus_tag="ACHKUN_003851"
     CDS             complement(383..1414)
                     /gene="rsmC"
                     /locus_tag="ACHKUN_003851"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO6662029.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1517..1930
                     /gene="holD"
                     /locus_tag="ACHKUN_003852"
     CDS             1517..1930
                     /gene="holD"
                     /locus_tag="ACHKUN_003852"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO6662030.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            1899..2345
                     /gene="rimI"
                     /locus_tag="ACHKUN_003853"
     CDS             1899..2345
                     /gene="rimI"
                     /locus_tag="ACHKUN_003853"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6662031.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2360..3037
                     /gene="yjjG"
                     /locus_tag="ACHKUN_003854"
     CDS             2360..3037
                     /gene="yjjG"
                     /locus_tag="ACHKUN_003854"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO6662032.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3128..4717
                     /gene="prfC"
                     /locus_tag="ACHKUN_003855"
     CDS             3128..4717
                     /gene="prfC"
                     /locus_tag="ACHKUN_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO6662033.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5110..5715
                     /gene="osmY"
                     /locus_tag="ACHKUN_003856"
     CDS             5110..5715
                     /gene="osmY"
                     /locus_tag="ACHKUN_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO6662034.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5842..6003
                     /locus_tag="ACHKUN_003857"
     CDS             5842..6003
                     /locus_tag="ACHKUN_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO6662035.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6125..7198
                     /locus_tag="ACHKUN_003858"
     CDS             6125..7198
                     /locus_tag="ACHKUN_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO6662036.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7195..7977
                     /locus_tag="ACHKUN_003859"
     CDS             7195..7977
                     /locus_tag="ACHKUN_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6662037.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1944 a         2126 c         2215 g         1885 t
ORIGIN      
        1 cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt
       61 ctaccaattc cgccaccttc gcataccatc aattcttaaa aagaattgct accacggagg
      121 cgcattctag tggttttcag cttttcgtca atagttaatt atcgccggag gtgtaattgc
      181 tggaaaaatg tccatcagga aactaacgtg caggttttgt aggtcggata aggcgttcac
      241 gccgcatccg acacggtatt tgggtgcaga tgccagatgc gacgcgggcg cgtcttatct
      301 ggcctacgaa gggctaacgt gcaggttttg taggttgtat aaggcgagca cgccgcatcc
      361 gacaaggctt gcggcgggat acttaacctt tcttcgcctg gcgggtcata atggcgcgat
      421 acaccttgaa gcgcccggtt tgcgcgataa cttcatggaa gccaaatgtc tcatccagca
      481 cgtccgggta aggcaggaag gcgttcgcta caattcgcag ctcgccgccg ctattaagat
      541 gacgcaccgc gccgcgaatc agcgtttgcg ccgcatccag gctggtttgc atcccatcgt
      601 ggaacggcgg gttggagatg atcatatcaa aacgaccttt cacctcggaa aagacgttgc
      661 tggcaaagac ttcaccttca acaccgttgg ccgcaagcgt cgcacggctg gcttctaccg
      721 ccggggcaga gacatcgcac aaggtgagac gaattttcgg cgaatggcgc gcaaaggcaa
      781 ctgaaagcac acccgcgcca cagccgacat ccagcacttt acctttcgtg tgcggggtta
      841 acgtcgagag cagcaactgg ctaccgacat ccagaccgtc gcggctaaac acgccaggca
      901 gcgttttgac cgtcaggcca tcgacgctgt attcgcccca gaatttatcc gcatcaaata
      961 ctggctgttt ttccagacga ccaaaataga ggccacagcg acgagcgctg tcgactttat
     1021 tcaacggcgc ataatctgcc agcatctgct cggcgctgcg cacgccgctg cggttctcgc
     1081 caacgacaaa aatatccgtc cccaccggca gcagagaaag taaattcatc aactggaact
     1141 gggcttccgg tttgttcttc ggccagtagt aaatcagcgt atcgcaatct gcgacgtcat
     1201 ccgccttggc gaccagacta aaacgggcgt tatcccccat ctggcggctt aatacctgcc
     1261 agtggtggaa ttgctgggta tgagcacggc tggccgcggt atctaaacgc gcgggcaggt
     1321 catcctgtaa gtctccggca aacagaatac ggctttgttc gaaatcatca ctgtgacgca
     1381 gcaagacttc acttgccggg gtaaatgcag acatggaatg ctcctcaatt gatactggcg
     1441 gcgattatag ccatatgttg gcgcggtatc gacgaatttg ctatatttgc gcccctgaca
     1501 acaggagcga ttcgctatga catcccgacg agactggcag ttacagcaac tgggcattac
     1561 ccagtggtcg ctgcgtcgcc ctggcgcgtt gcagggggaa attgccattg cgatcccggc
     1621 acacgtccgt ctggtgatgg tggcaaacga tcttcccgcc ctgactgatc ctttagtgag
     1681 cgatgttctg cgcgcattaa ccgtcagccc cgatcaagtg ctgcaactga cgccagaaaa
     1741 aatcgcgatg ctgccgcaag gcagtcgctg caacagctgg cggttgggta ctgacgaacc
     1801 gctatcactg gaaggcgctc aggtggcatc accggcgctc accgatttac gggcaaaccc
     1861 aacggcacgc gccgcgttat ggcaacaaat ttgcacatat gaacacgatt tcttccctcg
     1921 aaacgactga tttaccagcg gcttaccaca ttgaacaacg cgcccacgcc tttccgtgga
     1981 gtgaaaaaac ctttgccagc aaccagggcg agcgttatct caactttcag ttaacgcaaa
     2041 acggcaaaat ggcggcgttt gcgattacgc aagtggtgct ggatgaagct acattgttca
     2101 atattgcggt cgatcctgac tatcagcgtc agggattggg aagggcgctg ctggaacatc
     2161 tgatcgacga actggaaaaa cgcggcgtgg cgacgctctg gctggaagtc cgtgcctcaa
     2221 atgctgccgc cattgccctg tacgaaagtt taggctttaa cgaggcgacg attcgccgca
     2281 attactaccc caccacggac ggtcgcgaag acgccatcat catggcgttg ccaatcagta
     2341 tgtaagacaa ggtggaataa tgaagtggga ctggattttc tttgatgccg atgaaacgct
     2401 gtttaccttt gactcattca ccggcctgca gcggatgttt cttgattaca gcgtcacctt
     2461 taccgctgaa gattttcagg actatcaggc cgttaacaag ccactgtggg tggattatca
     2521 aaacggcgcg atcacttcat tacagcttca gcacgggcgt ttcgagagct gggccgaacg
     2581 gctgaaagtt gaagcaggct tgcttaacga tgcctttatt aatgcgatgg cggaaatctg
     2641 cacgccgctg ccgggcgcgg tttctctgct taacgccatt cgtggcaacg ccaaaatcgg
     2701 catcatcacc aacggcttta gcgccttgca gcaagtgcgt ctggaacgca cgggcctgcg
     2761 tgattacttc gatttgctgg tgatttccga agaagttggc gttgctaaac cgaataagaa
     2821 aattttcgat tatgcgctgg aacaggcggg caatcctgac cgttcacgcg tgctgatggt
     2881 tggcgacact gccgagtccg atattctcgg tggcatcaac gccgggcttg cgacctgctg
     2941 gctgaatgcg cacaatcgcg agcaaccaga aggcatcgcg cccacctgga ccgtttcttc
     3001 gttgcacgaa ctggagcagc tcctgtgtaa acactgattg cctccccccc gttgatgggt
     3061 aaaatagccg caatttttcg ttttcaacaa gcgcggcgcg atgccgctta ctcaagaaga
     3121 aagaattatg acgttgtctc cttatttgca agaggtggct aagcgccgca cttttgccat
     3181 tatttctcac ccggacgccg gtaagactac catcaccgag aaggtgctgc tgttcggaca
     3241 ggccattcag accgccggta cagtaaaagg ccgtggttcc aaccagcacg ctaagtcgga
     3301 ctggatggag atggaaaagc agcgtgggat ctccattact acgtctgtga tgcagtttcc
     3361 gtatcacgat tgcctggtta acctgctaga caccccgggg cacgaagact tctcggaaga
     3421 tacctatcgt accctgacgg cggtggactg ctgcctgatg gttatcgacg ccgcaaaagg
     3481 tgttgaagat cgtacccgta agctgatgga agttacccgt ctgcgcgaca cgccgatcct
     3541 cacctttatg aacaaacttg accgtgatat ccgcgacccg atggaactgc tcgatgaagt
     3601 tgagaacgag ctgaaaatcg gctgcgcacc gatcacctgg ccgattggct gcggcaagct
     3661 gtttaaaggc gtttaccacc tttataaaga cgaaacctat ctctatcaga gcggtaaagg
     3721 tcacaccatt caggaagtcc gcattgttaa agggctgaat aacccggatc tcgatgctgc
     3781 ggttggtgaa gatctggcgc agcagctgcg tgacgaactg gaactggtga aaggcgcgtc
     3841 taacgagttc gacaaagaat tgttccttgc gggcgaaatc actccggtat tcttcggtac
     3901 tgcgctgggt aacttcggtg tcgatcatat gctggatggc ctggtggagt gggctcctgc
     3961 gccgatgccg cgtcagactg atacccgtac cgtagaagcg agcgaagaca aatttaccgg
     4021 cttcgtattt aaaattcagg ccaacatgga cccgaaacac cgcgaccgcg tggcgtttat
     4081 gcgcgtggta tccggtaaat atgaaaaagg catgaagctg cgccaggtgc gcaccgcgaa
     4141 agacgtggtg atctctgacg cgctgacctt tatggcgggt gaccgttcgc acgttgaaga
     4201 agcgtatcct ggcgatatcc tcggcctgca caaccacggc accattcaga tcggcgacac
     4261 ctttacccag ggtgagatga tgaagttcac cggtattccg aacttcgcac cagaactgtt
     4321 ccgtcgtatc cgcctgaaag atccgctgaa gcaaaaacag ctgctcaaag ggctggtaca
     4381 gctttccgaa gagggcgcgg tgcaggtgtt ccgtccgatc tccaacaacg atctgatcgt
     4441 tggtgcagtt ggtgtgctgc agtttgatgt agtggtatcg cgcctgaaga gcgaatacaa
     4501 cgttgaagca gtatatgaat cagtcaacgt tgccaccgcc cgctgggtag aatgtgcaga
     4561 cgcgaagaaa ttcgaagagt tcaagcgtaa gaacgaaagc caactggcgc ttgatggcgg
     4621 cgataacctc gcttacatcg ctaccagcat ggtcaacctg cgcctggcgc aggaacgtta
     4681 tccggacgtt cagttccacc agacccgcga gcattaattc ttgcctccag ggcgcggtag
     4741 ccgctgcgcc ctgtcaattt cccttcctta ttagccgctt acggaatgtt cttaaaacat
     4801 tcacttttgc ttatgttttc gctgatatcc cgagcggttt caaaattgtg atctatattt
     4861 aacaaagtaa tgacatttct gacggcgtta aataccgttc aatgcgtaga tatcagtatc
     4921 taaagccgtc gattgtcatt ctaccgatat taataactga ttcagaggct gtaatggtcg
     4981 ttattcatca cccatcgctt ttgtgatggc gaccattgac ttctgtagag ggtgaagtct
     5041 ctccctattc agcaatgcaa cctcgtgttg ccaggctcaa attacgagca aacatacagg
     5101 aataaatcga tgactatgac aagactgaag atttcgaaaa ctctgctggc tgtaatgttg
     5161 acctctgccg tcgcgaccgg ctctgcctac gcggaaaaca acgcacagac taccaatgaa
     5221 agcgcagggc aaaaagtcga tagctctatg aataaagtcg gtaatttcat ggatgacagc
     5281 gccatcaccg cgaaagtgaa ggcggcgctg gtggatcatg acaacatcaa gagcaccgat
     5341 atctctgtca aaaccgatca aaaagtcgtg accctgagcg gtttcgttga aagccaggct
     5401 caggccgaag aggcagtgaa agtggcgaaa ggcgttgaag gggtgacctc tgtcagcgac
     5461 aaactgcacg ttcgcgacgc taaagaaggc tctgtgaagg gctacgcggg tgacaccgcc
     5521 accaccagtg aaatcaaagc caaactgctg gcggacgata tcgtcccttc ccgtcatgtg
     5581 aaagttgaaa ccaccgacgg cgtggttcag ctctccggta ccgtcgattc ccaggcacag
     5641 agcgaccgtg ctgaaagtat cgccaaagcg gtagatggtg tgaaaagcgt taaaaatgat
     5701 ctgaaaacta agtaattcgt cgtaattcgt cctcccgaac tttgtcggga ggcgcgatgt
     5761 gcaccacact aaaaatatcg cattgagtga ttttcacgct catatcaagc ggtgacatta
     5821 actatggtaa aggagacgct tatgtttcgt tggggcatca tatttctggt tatcgcgtta
     5881 atcgccgccg cacttgggtt tggtggtctg gccggtaccg ctgcaggcgc agctaaaatt
     5941 gtctttgtcg tcgggattat tctgttcctg gtgagtttgt tcatgggccg aaaacgaccc
     6001 tagatttcag accattaaga aaattttatc caaagccagt ccagcggact ggcttttgcg
     6061 gttttagcga ataataaatt gcgttacttt gtcattcttt cacaacaaaa acaggaaggc
     6121 agaggtgggg cagcgaatac ctgtaacgct tggtaatatt gcgccgttgt cgctaaggcc
     6181 gttccagcct ggacgaatag ctctggtgtg cgaaggcggc ggacagcgtg gaattttcac
     6241 ggctggcgtg ctggatgagt ttatgcgcgc gcagtttaat cctttcgatc tttatctcgg
     6301 cacatctgcc ggggcgcaga acctctcggc gtttatctgc aatcagcccg gttacgcgcg
     6361 caaagtcatc atgcgctata ccacaaaacg cgaatttttc gatccattgc gctttgtccg
     6421 tggaggaaat cttatcgatc tcgactggct ggtggaggcc accgcaagcc agatgccgtt
     6481 gcaaatggat accgccgcgc ggttgtttga cagcggcaaa tcgttttata tgtgcgcctg
     6541 tcgtcaggat gactacgcgc ctaattactt tttaccaacc aaacaaaact ggctggatgt
     6601 gattcgcgcc tccagtgcga tacctggctt ttatcgtagc ggagtgtcgc tggaaggcat
     6661 taactacctg gatggcggga tcagtgatgc gattccggtt aaagaggcgg caaggcaggg
     6721 cgctaaaacg ttggtcgtca tccgcactgt gccgtcacaa atgtactaca cgccgcagtg
     6781 gttcaaacgc atggaacgct ggctgggtga cagtagcctg cagccgctgg tcaatctggt
     6841 gcagcatcat gaaaccagct atcgtgacat tcagcaattt attgagaaac caccgggcaa
     6901 gctgcggata ttcgaaattt atccaccgaa accattacat agtatcgcgc ttggcagtcg
     6961 gattccggcg ctgcgtgaag actataaact tgggcgttta tgcggtcgtt atttcctcgc
     7021 cacggttggc aagctattaa ctgaaaaagc gccgcttacc cgccatctgg tgccagtggt
     7081 gacgccggaa tcgatcgtta ttccgcctgc gccagtcgcc aacgatacgc tggttgccga
     7141 agtgagcgat gctccgcagg cgaacgaccc gacatttaac aatgaggatc tggcttgatt
     7201 tgccgtttta tcgataccca ctgccatttt gatttcccgc cgtttagtgg cgatgaagag
     7261 gccagcctgc aacgcgcggc acaagcgggc gtaggcaaga tcattgttcc ggcgacagag
     7321 gcggaaaatt ttgcccgcgt gcaggcgtta gcggaaaaat atcaaccgct gtatgccgca
     7381 ttggggttgc atccggggat gttggaaaaa catagcgatg tgtctcttga tcagctacag
     7441 caggcgctgg aaaggcgtcc ggcgaaggtg gtggcggtgg gggagatcgg tctggatctc
     7501 ttcggcgacg atccgcaatt tgagaggcag cagtggttac tcgacgaaca gctgaaactg
     7561 gcgaaacgct acgatctgcc ggtgatcctt cattcccggc gtacacacga caaactggcg
     7621 atgcatctta aacgtcacga tttaccgtgc actggcgtgg ttcacggttt ttccggcagc
     7681 ctgcaacagg ccgagcggtt tgtacagctg ggctacaaaa tcggcgtagg cggtactatc
     7741 acctatccac gcgccagtaa aacccgcgat gtcatcgcaa aattaccgct ggcatcgttg
     7801 ttgctggaaa ccgacgcgcc ggatatgccg ctcaacggct ttcaggggca gcctaaccga
     7861 ccggaacagg ctgtgcgggt gtttgacgtg ctttgcgagt tgcgcccgga accagaagat
     7921 gagattgccg aagtgttgct taataatacg tatgcgttgt ttagcgttag tgggtagttt
     7981 acccttcgta ggcagcatcc ggcagtcggc gcataatgcc tgatgcgacg cttgtcgcgt
     8041 cttatcatgc ctacaggttt gtgccgaacc gtaggccgga taaggcgttc acgccgcatc
     8101 cggcagttgg cgcacaatgc ctgatgcgac gcttgtcgcg tcttatcatg cctacaggtt
     8161 tgtgccgaac
//
LOCUS       DAZFRJ010000182         8169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000182 DAZFRJ010000000
VERSION     DAZFRJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.184"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..527
                     /gene="ftnA"
                     /locus_tag="ACHKUN_003860"
     CDS             30..527
                     /gene="ftnA"
                     /locus_tag="ACHKUN_003860"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO6662038.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(565..804)
                     /gene="yecH"
                     /locus_tag="ACHKUN_003861"
     CDS             complement(565..804)
                     /gene="yecH"
                     /locus_tag="ACHKUN_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO6662039.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            995..2206
                     /gene="tyrP"
                     /locus_tag="ACHKUN_003862"
     CDS             995..2206
                     /gene="tyrP"
                     /locus_tag="ACHKUN_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO6662040.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2268..2933)
                     /gene="yecA"
                     /locus_tag="ACHKUN_003863"
     CDS             complement(2268..2933)
                     /gene="yecA"
                     /locus_tag="ACHKUN_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO6662041.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3129..3215)
                     /locus_tag="ACHKUN_003864"
     tRNA            complement(3129..3215)
                     /locus_tag="ACHKUN_003864"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3179..3181),aa:Leu,seq:taa)
     gene            complement(3228..3301)
                     /locus_tag="ACHKUN_003865"
     tRNA            complement(3228..3301)
                     /locus_tag="ACHKUN_003865"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3267..3269),aa:Cys,seq:gca)
     gene            complement(3356..3431)
                     /locus_tag="ACHKUN_003866"
     tRNA            complement(3356..3431)
                     /locus_tag="ACHKUN_003866"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3396..3398),aa:Gly,seq:gcc)
     gene            complement(3583..4131)
                     /gene="pgsA"
                     /locus_tag="ACHKUN_003867"
     CDS             complement(3583..4131)
                     /gene="pgsA"
                     /locus_tag="ACHKUN_003867"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO6662042.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4188..6020)
                     /gene="uvrC"
                     /locus_tag="ACHKUN_003868"
     CDS             complement(4188..6020)
                     /gene="uvrC"
                     /locus_tag="ACHKUN_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO6662043.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6017..6673)
                     /gene="uvrY"
                     /locus_tag="ACHKUN_003869"
     CDS             complement(6017..6673)
                     /gene="uvrY"
                     /locus_tag="ACHKUN_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO6662044.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6815..6931
                     /locus_tag="ACHKUN_003870"
     CDS             6815..6931
                     /locus_tag="ACHKUN_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6662045.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            6969..7145
                     /gene="yecU"
                     /locus_tag="ACHKUN_003871"
     CDS             6969..7145
                     /gene="yecU"
                     /locus_tag="ACHKUN_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO6662046.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            7132..7356
                     /gene="yecF"
                     /locus_tag="ACHKUN_003872"
     CDS             7132..7356
                     /gene="yecF"
                     /locus_tag="ACHKUN_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO6662047.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7424..8146)
                     /gene="sdiA"
                     /locus_tag="ACHKUN_003873"
     CDS             complement(7424..8146)
                     /gene="sdiA"
                     /locus_tag="ACHKUN_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO6662048.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2024 a         2095 c         1916 g         2134 t
ORIGIN      
        1 aacaaatata acctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta
       61 atgagcagat gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt
      121 gcagctatca taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga
      181 tgacgcatat gcagcgtctg tttgattacc tgactgatac cggcaattta ccgcgtatta
      241 ataccgttga atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct
      301 ataaacacga acaattaatc acgcagaaaa ttaacgaact ggctcatgct gcaatgacca
      361 atcaggacta cccaacattt aatttcctgc aatggtatgt ttctgagcag catgaagaag
      421 agaaactgtt caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc
      481 tgtattttat cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctcaggcgg
      541 caggttttct gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa
      601 accttcttct gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc
      661 agtcatccct tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat
      721 tgctgcttcc agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac
      781 ttcatgaccg tgaatagagt ccatcgtccc tcctcaaaaa aagcctagcg tagcgattgc
      841 cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt tttattgtac atttatattt
      901 acaccatatg taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat
      961 atcactcata aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt
     1021 ttttatcgtg gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc
     1081 cggtgttggt tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta
     1141 cacggcgcta ttactgctgg aggtgtacca gcatgttccg gcagataccg gtcttggcac
     1201 gctggcaaaa cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt
     1261 cttaatgtat gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag
     1321 catcagcgac tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt
     1381 tgttgccggt ggcgtggttt gtgttggaac atcgctggtc gatttattta accgttttct
     1441 gttcagcgcc aaaattattt ttctagtggt gatgctggta ttgctgctac cgcatattca
     1501 caaagtgaat cttttaaccc tgccgttgca acaggggctg gctctgtcag caatcccggt
     1561 gatttttacc tcatttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg
     1621 caacgttcgt aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata
     1681 tattttctgg caggtggcaa cgcttggcag cattgattca acaaccttta tgggattgct
     1741 ggctaatcat gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc
     1801 gcatgttgag ctggcagtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg
     1861 cgttgcgtta ggcttatttg attatctggc tgatttattt cagcgttcaa ataccgttgg
     1921 tggacggttg caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta
     1981 tccacgagga tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt
     2041 gattatccct tcgctgttga cctggcaaag cagaaagcac aatcctcagg cgggttaccg
     2101 ggtgaaaggt ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg
     2161 cgtgcaattt ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa
     2221 attccttatt gggtgccaga attaacgctg acacccaatt tgacctctta atgcaggcag
     2281 cactgcttaa atttcttacc actaccgcac gggcaaggat cgttacgccc cggtttctct
     2341 tctgctttga tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt
     2401 agatcaagcg ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt
     2461 ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt
     2521 tttaacgaat cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag
     2581 caccactcct caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc
     2641 tcaaactgct ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc
     2701 agattcataa agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca
     2761 ccccacacgg caaccagcca ctgttccggt tcaatctctt gcggagaact caacaccgcc
     2821 gtcaataaac cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc
     2881 agaatatcgt ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt
     2941 tccttgtgga tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact
     3001 ttttgagtgg ctttgctggt gattaaaaat tagggagggt gtaacgacaa gttgcaggca
     3061 caaaaaaacc acccgaaggt ggtttcacga cactgcttat tgctttgatt ttattcttat
     3121 ctttcccatg gtacccggag cgggacttga acccgcacag cgcgaacgcc gagggatttt
     3181 aaatcccttg tgtctaccga ttccaccatc cgggctcggg aagaaagtgg aggcgcgttc
     3241 cggagtcgaa ccggactaga cggatttgca atccgctaca taaccgcttt gttaacgcgc
     3301 caaattcttc aggcctttca gccagacatc cgcttgacgc cgatgtcttt taaactggag
     3361 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
     3421 gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat
     3481 cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt
     3541 ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac gatcactgat caagcaaatc
     3601 tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa
     3661 gaaaagtgca ataccggcgt actcaaccca aatgttcgga cgccacagca gccaggccaa
     3721 cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg
     3781 tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat
     3841 cgttgccgcc ggtagggtca cccaccagct gtgataatgc tcggttacca gcaccatggc
     3901 gatagccacg agaactttat ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg
     3961 gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat
     4021 gagcgcggcg gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg
     4081 gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg
     4141 taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac
     4201 gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctcg
     4261 acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccgacgt
     4321 tttggcccga cgccttcaat ggtttccagg gagctggtat ttttgacctt cgcccgtttt
     4381 ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc
     4441 agcgcgggag aatctggcgg caaactgaat ccctcacctt ccggctcaaa gaacagtgtt
     4501 tccagcccag ccttacgatc tactcctttg gcaacgccaa gtagcagtgg atgattttta
     4561 tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg
     4621 ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga
     4681 cgcagcacct gattcatcgc cgcataatca tcgcccggcg tgatgcctgt aatgttatag
     4741 cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt
     4801 tgttcgccca tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc
     4861 aacacgctgg caagcgcggt cagtcgctgg tgaacggtag attgctgcga aagtttgctg
     4921 gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat aacgcgccct atcgccgcga
     4981 ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc
     5041 gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg
     5101 ccttgtaaat agaactggcc tacgaaggtt tctaccacct cgctcagttc cgtaccgcca
     5161 ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg
     5221 tggacacaag ccatgcccgc atcgaacgcc acaccaataa cgtcgaggtc gtcgccggta
     5281 ttggaaacga attgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt
     5341 gcagcttctt caaactccag attctggctg gcagtttcca tacgactaat gagttgcgta
     5401 agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat
     5461 tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctggtattgc
     5521 agacacggac gcgagcgatt gcgataaaca ctattttcgc actggcgaat ggggaaaatc
     5581 ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat
     5641 tcacctttgg catgcttcgc accacgatgc atcgccagac gcgggtgggt atcgccactc
     5701 aggaagataa aaggataaga tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag
     5761 agtttgatgt agttgtgttc cagcaacagc gcttcggttt ctgtatgagt aaccgttaca
     5821 tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg
     5881 aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca
     5941 ccagcatcgt acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt
     6001 gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa
     6061 ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc
     6121 ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct
     6181 ggcccttggt gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc
     6241 tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgatg tcagaagcaa
     6301 tgtaacgctg ccctgaatag acagaacgaa tcgcactcac gacttcctgc ggagccgcgc
     6361 ctttgctgag gtagcccgca gcaccggcct gcatgacttt cgctggtaaa gggttttctg
     6421 tatggacggt aagcatgatg attttgacat cagctgtgga acgcgcgatt ttacgcgtcg
     6481 cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg
     6541 cccgacacca cttaacggcg tcttcaccgc acgatgcctc accgacgact tttataccct
     6601 ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta
     6661 gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt
     6721 cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt
     6781 tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt
     6841 accggatcgt ttcacagtaa gccctgcgac gaaatgtgac aaaaattacc tttattcagc
     6901 aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa
     6961 aaacattcat gataaaaata tttattggtc attatattaa cgcatttaat agcaccgttg
     7021 acgatacgct caaaaaacaa ccactgctat ttttagcaaa gcttatggta tactccgccg
     7081 ccttaacatt attcaccgca aattttcatt gcaacatgac gaggaaaata aatgagtacg
     7141 cctgattttt ctactgccga gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa
     7201 gcgatgctga cgctgatgct gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta
     7261 aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt attttccaaa
     7321 acggttaagc aaattaaaca agcctaccgt cagtaatata aaaccggctg atagcgtgcc
     7381 tttcagccgg tttttgcatc tggcacgcag gacagaaaag agatcaaatt aagccagtag
     7441 cggccgcgta acaggcaacc tgggtcttat tcggtgcatt gatttttttc tgcatgtttt
     7501 tctgatggaa attgaccgta ttctcagaga ttgacaaaat catcgctatc tctgctgatg
     7561 tcttcccttc cgccgtccac ctcagaattt ctttttcgcg cttgctgaaa ttcatctctg
     7621 gcgtcatcac tatttcatca tttaaacgca tcagagccat cagactttcg cgcaccagta
     7681 actgcatttt taattgcagt tcatcactaa gaatgggtat ttcgcgcgcg ctgcaacggg
     7741 aaaaggacaa aaagcccagc gcccggtttg gcagcattaa atactgagtg acaccgcggc
     7801 gtaaaccatg tgcgcgcgcg gcttcccata acggctgtgc ttcgttgaat aagtcatcat
     7861 tccacattag atggccctga ctaaagtttt cagggttcag caccggatca attgcgagaa
     7921 agttttttgc ctgataataa ctaacccacg cctcagggta attggtgtag aaagccactt
     7981 taggtcgcgt gaatggtacc gggtggcgaa cacataacga atagtaatcg tactccagct
     8041 gctgagcctg aagctctatt tcatggtaga cctcttctgc ggcctccatc ctctgaaaac
     8101 gcaacagcat cgtgcgacgc cagctgaaaa aatccgtatc ctgcatagta aaccgcaacg
     8161 cccctgaga
//
LOCUS       DAZFRJ010000183         8134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000183 DAZFRJ010000000
VERSION     DAZFRJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.185"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..905)
                     /locus_tag="ACHKUN_003874"
                     /pseudo
     CDS             complement(1..905)
                     /locus_tag="ACHKUN_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1202..1804
                     /locus_tag="ACHKUN_003875"
     CDS             1202..1804
                     /locus_tag="ACHKUN_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFO6662049.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            1801..2403
                     /locus_tag="ACHKUN_003876"
     CDS             1801..2403
                     /locus_tag="ACHKUN_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFO6662050.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            2415..6056
                     /gene="brxC"
                     /locus_tag="ACHKUN_003877"
     CDS             2415..6056
                     /gene="brxC"
                     /locus_tag="ACHKUN_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFO6662051.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLESSRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKADILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWINMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELCKKQEAQLRAANEAKRAADS
                     EQTYVVVEKTVIQPVPKKTHLVNVASEMRNAAGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            6102..>8134
                     /gene="pglX"
                     /locus_tag="ACHKUN_003878"
     CDS             6102..>8134
                     /gene="pglX"
                     /locus_tag="ACHKUN_003878"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFO6662052.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLEHGFRMLSHPETPTAFEVLDHVPEVAEALLPENKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHVMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDNIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENTALTQTNSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQEGDFQQKSAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGSKYQSLSVKKHLKDNFTGYDKDLFSAFIVRNLELSKQGAQLGFMTPFVWMFI
                     TTYEELRKKLIEQEIITSLVQLEYSGFDGATVPICTFTLQKGHINHFTGSYIRLSDFR
                     GASNQGPKTLEAIQHHNCGWFYEAQPDNFKKIPGTPIAYWLGQNINIFDNDKIA"
BASE COUNT         2268 a         1905 c         1909 g         2052 t
ORIGIN      
        1 cattgattag aaatggcgac aatttcacca gaaggaagtg taatcaatgc atcttcgttg
       61 atatgatagc gactcttttg cttctctggt atctgatcga taagtataac cgtacgtttt
      121 ttaaagtctt cacttaatcc atttattaaa tcattataac ttgcagggtt aaattgtttt
      181 atacaatcgc gtaataactc aagcgctaac ttacgtttat taagtttttt atctttgcat
      241 atataccacg tatagccctt ctcttttttc tgactgctaa ttagctgctc atcacgtttt
      301 tcacgaaact taacttgata ttcttccaat tcaggaaccg gtatgatttg ctctgcattg
      361 atcagaacat catcattaaa tctataaggc gtcagacgaa cacatcgaat gtctacccct
      421 ttatctctta accaaatcgc agtggtagtc agttccttgc taaaatccga tgaagccaaa
      481 acgattctga tatctttacc aaaatcaaca agctcagttt catccagctc aacaaattca
      541 agtatttttt ctttcgcatt ttttctttcg catcggcatc gcggttttgt tttttcaaat
      601 acgtctgaaa atactcacat gctttcgcaa aactcattgt gtaaatcatt gccgcatatc
      661 gtagtgcctg taactccata tgcgtccccg tttcatcacg ttttaattcg ataaccacta
      721 tatttgcctg tttatcgatt gccaataaat caattcgccg atggctatct tcccagtcag
      781 aaatcaccag acaatcagga gcaataatat ctatattttt tgattgcttc ctgtaatcct
      841 tccagagtaa aggtcgtttt ttctaaagca tgtggttctt tatttgcgat attgtacagc
      901 accacgttca tcttcctttt ttaactgatt ttacagagac tttaatacag ttaaaatttt
      961 atttcctgag ctgtaatcga ttaagttgat gcatttaatg ggaatgatat agggtcattt
     1021 ccagtctcac ttatagaaat ggctaaagca tgactctcgc caaaaccgtt tatgtgttgt
     1081 acataacgcg atcatccctc tcacaaattg ccttttctca tggcatctcg cccggtcccc
     1141 cattacaatc actttttgtt ttttgcgagc tgcattccag tcttcagagg gtttttcgat
     1201 gattaaaaat gacaaggcat ggataggaga cttgctgggc ggaccgctca tgagcaggga
     1261 aagccgcgtc attgccgaac tgttgctaac cgatcccgat gaacagacat ggcaagagca
     1321 aattgttggc cacaacattt tacaagcctc ttctcctaac accgcaaaac gttacgcggc
     1381 aacaatcagg cttcgcctga acacgctgga taaaagcgcg tggacattga ttgccgaagg
     1441 tagtgaacgg gaacgccaac aacttctgtt tgtggctctg atgctacatt cgccggtagt
     1501 taaggatttt ctggctgaag tggtgaacga tctgcgcagg cagttcaagg aaaagttgcc
     1561 tggcaatagc tggaacgaat ttgtgaatag ccaggttcgc ctacatccgg tactcgccag
     1621 ctactcagat tcatctattg caaaaatggg aaacaatctg gtgaaggcgc ttgctgaagc
     1681 gggttatgtg gatacgcccc gcagacgtaa cctgcaggca gtttaccttt taccggaaac
     1741 tcaggcagtg ttacagcgcc tgggacaaca ggacttgata tctattctgg agggaaaacg
     1801 gtgatagatc ccgttcttga atatcgcctg tctcaaatcc agagtcgcat taacgaagat
     1861 cgcttcctca aaaataacgg ctccggaaat gaaattggtt tttggatctt tgattatccc
     1921 gcgcagtgcg aactgcaggt acgggagcat ttgaaatatc tgctccggca tctggaaaag
     1981 gaccataaat ttgcctgtct gaatgtcttc caaatcatca tcgatatgct caatgaacgc
     2041 ggccttttcg agcgcgtctg ccagcaggaa gtcaaagtgg gtactgagac gctgaaaaag
     2101 cagcttgctg gtccgttaaa tcagaaaaag atcgctgatt ttatagcgaa aaaagtcgat
     2161 ctggctgccc aggattttgt cattcttacc ggcatgggca acgcctggcc attagtacgc
     2221 ggtcatgaac tgatgagtgc cttgcaggat gtcatggggt tcaccccact gctgatgttt
     2281 tatcctggca cctacagcgg gtacaacctt tccccgctca cagacaccgg ttcacaaaat
     2341 tattatcgcg ctttcagact ggtaccagat acgggacccg cagcaacatt gaatcctcaa
     2401 tgaagagcat aacaatgaat attgaacaga tttttgaaaa acctctaaaa cgaaatataa
     2461 acggggtagt caaagcagag caaaccgatg atgccagcgc gtacatcgag ttagatgaat
     2521 atgtcatcac ccgcgaactg gaaaaccatc ttcgccattt cttcgaatcc tatgttcctg
     2581 ccactggccc ggaacggatc cgtatggaaa acaagatcgg cgtatgggtt tcaggcttct
     2641 tcggttcagg taaatcgcac tttattaaga ttctttctta tcttttatct aaccgcaaag
     2701 ttacacataa cggtacggaa cgtaatgctt actccttctt tgaagataaa atcaaagatg
     2761 cattattcct tgccgatatt aacaaagcgg tgcattaccc gactgaagtc attctgttca
     2821 atattgattc gcgtgccaac gtagatgaca aagaagatgc cattcttaaa gtcttcctga
     2881 aagttttcaa cgaacgcatt ggatactgcg ctgattttcc gcatattgcc catcttgagc
     2941 gcgagctgga taaacgcggt cagtatgaaa cctttaaagc cgcgtttgcc gatatcaatg
     3001 gctcgcgctg ggaagacgag cgcgacgctt actacttcat cagcgatgac atggcacaag
     3061 cattaagcca ggccacgcag cagagtcttg aatcctcccg ccaatgggtg gaacaactcg
     3121 acaaaaactt cccgctggat atcaataatt tttgccagtg ggtaaaagag tggctggatg
     3181 acaatggtaa gaacatcctc tttatggtgg atgaagtcgg tcagttcatt ggcaaaaata
     3241 cgcaaatgat gctgaagctg cagactatta ctgaaaacct tggggtaatt tgcggtggcc
     3301 gcgcatgggt tatcgtgact tcgcaggccg atatcaacgc ggcaatcggt ggtatgagca
     3361 gtcgcgacgg acaggacttc tccaagatcc aggggcgctt ctctacacgc ctgcaacttt
     3421 ccagctctaa cacatcagaa gttatccaga aacgtttgtt ggtaaagact gacgaagcaa
     3481 aagcggcact ggcaaaagtg tggcaagaga aagccgatat cctgcgtaac cagctggctt
     3541 ttgaccctac aacaactact gcactacgtc cttttaccag cgaagaagag ttcgttgaca
     3601 actacccgtt tgtcccgtgg cactatcaga ttctgcaaaa agtgtttgaa tctattcgaa
     3661 cgaaaggtgc agcgggtaaa caattggcca tgggtgagcg ttctcagctg gaggcattcc
     3721 agacggcggc gcagcaaatc tcagcgcaag ggctggattc tctggtgcct ttctggcgct
     3781 tctatgccgc cattgagagc ttcctggaac ctgccgttag ccgcaccatc actcaggctt
     3841 gccagaatgg cattcttgat gaattcgatg gcaacctgct taaaacgctg ttcctgatcc
     3901 gctatgtgga aacgctgaaa agcaccctgg ataacctggt cacattgtct atcgatagga
     3961 tcgatgccga taaagttgag ttgcgccgcc gggtcgaaaa aagtctgaac acgcttgaac
     4021 gcctgatgct cattgcgcgc gttgaagata aatatgtgtt cctgaccaac gaagaaaaag
     4081 agatcgaaaa cgagatccgt aacgttgatg tcgatttctc tgcgatcaac aaaaaactgg
     4141 catcgatcat ctttgatgac attctgaaaa gccgtaaata tcgttatccg gctaacaagc
     4201 aagactttga tatcagtcgc ttcctgaacg gacacccatt agacggcgca gtgcttaacg
     4261 atctggtagt gaagatcctg acccctaaag atccgaccta ttcgttctat aacagcgatg
     4321 cgacctgtcg cccttatacg tcagaaggcg acggctgtat tttgattcgt ctgcccgaag
     4381 agggccgtac ctggagcgat attgatttag tcgtccagac tgaaaagttc ctcaaagata
     4441 acgccgggca acgtccggaa caggcaaccc tgctctcaga aaaagcgcgt gaaaacagca
     4501 accgggaaaa attactccgt gttcagttgg aatcactact tgcagaagca gatgtctggg
     4561 cgattggcga gcgcttaccg aaaaaatctt ccacaccatc gaacattgtc gatgaagcct
     4621 gccgttacgt gattgaaaac accttcggca agctgaagat gctgcggcct tttaacggcg
     4681 acatctcccg tgaaattcat gcattactga cggttgagaa cgacaccgaa ctggatctcg
     4741 gtaacctcga agagtccaac cccgacgcca tgcgcgaggt agaaacctgg atcaacatga
     4801 atatcgaata caataaacct gtgtatttac gcgatattct gaaccatttc gcgcgtcgcc
     4861 cttatggctg gcccgaagac gaagtgaaac tgctggtagc ccgtctggcc tgcaaaggta
     4921 agttcagctt tagccagcaa aacaacaacg tcgagcgaaa acaggcgtgg gagttattta
     4981 ataacagccg ccgccatagc gaattgcgtc tgcataaagt tcgccgtcat gatgaagcgc
     5041 aggtgcgtaa agccgcgcaa accatggctg acatcgctca gcagccgttt aacgaacggg
     5101 aagagccggc gctggttgaa catattcgtc aggtatttga agagtggaag caagagctga
     5161 acgtattccg cgccaaggca gagggcggaa acaatccggg gaaaaacgag attgaatccg
     5221 gtctgcgcct gcttaatgcc attcttaatg agaaagaaga ttttgccctg atcgaaaaag
     5281 tctcatcgct gaaagatgaa cttctggatt tcagtgaaga ccgtgaagat ttggtcgact
     5341 tctaccgtaa gcaattcgcc acctggcaaa aactgggtgc tgcgctgaat ggcagcttta
     5401 aatctaaccg cagcgcgctg gaaaaagacg ccgcagcggt taaagcgctg ggtgagctgg
     5461 aaagcatctg gcaaatgccg gaaccttata agcatctcaa tcgcatcacg ccgttgattg
     5521 aacaggtcca gaacgtcaac catcagttag tcgaacagca ccgccagcac gccctcgaac
     5581 gcattgacgc ccgcattgag gaaagccgtc aacgcttgct ggaagcgcac gccacgtcag
     5641 aactgcaaaa cagcgttctg ctgccgatgc aaaaagccag aaaacgcgct gaggtcagcc
     5701 agtcgattcc ggaaattttg gcggaacagc aagagacaaa agcgctgcaa atggatgcag
     5761 ataaaaagat taatctgtgg atcgatgagc tgtgtaaaaa gcaagaagca caactccggg
     5821 cagcaaatga agctaaacgc gctgccgact cagaacagac ttatgttgtg gtggaaaaaa
     5881 ccgttatcca accggtaccg aaaaaaacgc atctggtgaa tgtcgccagt gagatgcgta
     5941 acgccgccgg tggtgaggtt ctggaaacca ccgaacaggt ggaaaaggcg ctcgacacgt
     6001 tacgcacaac gctgctggcc gccattaaag caggcgatcg aattcgcctt cagtaactcc
     6061 catttcaggg cagcactctg ctgccctttg caggattttc tatgaatact aataacatta
     6121 aaaaatatgc cccacaggcc cgtaacgatt tccgcgatgc ggtgattcag aagctaacga
     6181 cgcttgggat cgctgcagat aaaaaaggca atttgcagat tgccgaggcc gaaaccattg
     6241 gcgagaccgt gcgttacggt cagtttgatt acccgttatc gacccttccc cgccgcgaac
     6301 ggctggtaaa acgcgcccgt gagcagggtt ttgaggtgct ggttgagcac tgcgcctaca
     6361 cctggtttaa ccgcttatgt gcaattcgct atatggagct acacggttat cttgagcacg
     6421 gcttccgtat gttgtcccac ccggagacgc cgaccgcgtt tgaggtgctg gatcatgtgc
     6481 cggaagtggc agaagccctg ctgccggaaa ataaggcgca gctggttgaa atgaagcttt
     6541 ccggtaatca ggacgaagcc ctgtaccgcg aactgctgct ggggcagtgc cacgccctgc
     6601 accacgtgat gccgttcctg tttgaagcgg tagatgacga agcggaactg ctgttgccgg
     6661 ataacctgac ccgtaccgac tctattctgc gtgggctggt tgataatatt cctgaagaag
     6721 actgggagca ggtagaggtt atcggctggc tgtatcagtt ctatatttcg gaaaagaaag
     6781 atgctgtgat tggcaaagtg gtgaagagcg aagatattcc tgccgccacc cagctgttta
     6841 cgccaaactg gattgtgcag tatctggtac agaactccgt tggccgccag tggttgcaga
     6901 cctacccgga ctcgccgctg aaagacaaaa tggagtacta cattgagcct gcggagcaaa
     6961 cgccggaagt gcaggcacag ctggcggcga ttaccccagc cagcattgaa cccgaaagta
     7021 ttaaagtgct cgacccggcc tgcggttccg gtcatatttt gattgaagcc tataatgtgc
     7081 tgaaaaatat ctacgaagag cgtggttatc gcgggcgtga tattccacaa ctgattctgg
     7141 aaaataatat ttttggtctt gatatcgacg accgcgcagc ccaactttcc ggttttgcat
     7201 tattaatgat ggcgcgtcag gatgaccgca gaatatttac ccgcgatgta cgtctgaata
     7261 ttgtctcttt gcaggaaagc ctgcatctgg atatcgccaa actctggcag caactgaatt
     7321 tccaccagca ggtacaaacc ggcagtatgg gggatatgtt tgctgaaaat accgcgttaa
     7381 cccaaactaa cagcgcagaa tatcagctgc tgatgcgcac gttgaaacgc tttgtgaatg
     7441 caaaaacgct gggatcgctg attcaggtgc cgcaggaaga agaagcggaa ctgaaagtat
     7501 ttctggacgc gctgtatcgc ctggaacagg aaggcgattt ccagcagaag tcggcggcga
     7561 aagcgtttat tccgtttatt cagcaggcgt ggattctggc acagcgatat gatgcggtgg
     7621 tggcgaatcc gccgtatatg ggaagcaaat accaatcatt aagtgtaaaa aaacatctta
     7681 aagataattt tactggttat gataaggatt tattttcagc ctttatagtc agaaacttag
     7741 aactttcaaa acaaggggct caacttggat tcatgacgcc atttgtctgg atgtttatca
     7801 ctacgtacga agaattaaga aaaaaactga tagagcaaga aattattaca tcgcttgtgc
     7861 agcttgaata ttcaggcttt gacggtgcaa ctgtgccaat ttgcacattc acattacaga
     7921 aaggtcatat taaccatttt actggtagct atatccgcct ctctgatttt cgtggtgcat
     7981 caaaccaagg tcccaaaact cttgaagcga tacaacacca taattgtgga tggttttatg
     8041 aagcgcagcc agacaatttt aaaaaaatac caggaacacc aatagcctat tggctaggcc
     8101 agaatataaa tattttcgat aatgacaaaa tagc
//
LOCUS       DAZFRJ010000184         8132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000184 DAZFRJ010000000
VERSION     DAZFRJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.186"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..279
                     /locus_tag="ACHKUN_003879"
     CDS             <1..279
                     /locus_tag="ACHKUN_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662053.1"
                     /translation="NELTRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNST
                     CIYKSRHKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            502..999
                     /gene="ubiC"
                     /locus_tag="ACHKUN_003880"
     CDS             502..999
                     /gene="ubiC"
                     /locus_tag="ACHKUN_003880"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO6662054.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1012..1884
                     /gene="ubiA"
                     /locus_tag="ACHKUN_003881"
     CDS             1012..1884
                     /gene="ubiA"
                     /locus_tag="ACHKUN_003881"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO6662055.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2039..4462)
                     /gene="plsB"
                     /locus_tag="ACHKUN_003882"
     CDS             complement(2039..4462)
                     /gene="plsB"
                     /locus_tag="ACHKUN_003882"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO6662056.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4633..5001
                     /gene="dgkA"
                     /locus_tag="ACHKUN_003883"
     CDS             4633..5001
                     /gene="dgkA"
                     /locus_tag="ACHKUN_003883"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO6662057.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5111..5719
                     /gene="lexA"
                     /locus_tag="ACHKUN_003884"
     CDS             5111..5719
                     /gene="lexA"
                     /locus_tag="ACHKUN_003884"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO6662058.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5792..7117
                     /gene="dinF"
                     /locus_tag="ACHKUN_003885"
     CDS             5792..7117
                     /gene="dinF"
                     /locus_tag="ACHKUN_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO6662059.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7233..7442
                     /locus_tag="ACHKUN_003886"
     CDS             7233..7442
                     /locus_tag="ACHKUN_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO6662060.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7484..7999)
                     /gene="zur"
                     /locus_tag="ACHKUN_003887"
     CDS             complement(7484..7999)
                     /gene="zur"
                     /locus_tag="ACHKUN_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO6662061.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1827 a         1990 c         2238 g         2077 t
ORIGIN      
        1 aatgaattaa cacgaaagga taatatcttt atcctgttct cctctgccga tgatgggcca
       61 gttatggtgg taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat
      121 tggaattcca cgtgtatcta taaatccaga cataagatgt tgcctattaa tcttactcag
      181 gaaacacttt ttagctccaa atctcatggt aaatatgcgc ttttcccaat ttttactgcg
      241 agttggcgag ctactcgtat aaagaatata ggtatttaag taaaggaaaa catcaccgtt
      301 cctggcatca tgggcggtga tgcccctacg gttgccctcg ccagcacggg catcggcaaa
      361 gcgtaaggtt caacagcggt ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta
      421 gatcacttaa tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg
      481 ttatgtaacg gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc
      541 tattttaaag agatccctgc cctggatccg caactgctcg actggctgtt gctggaggat
      601 tccatgacaa aacgttttga acagcaggga aaaacggtaa gcgtgacgat gatccgcgaa
      661 gggtttgtcg agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt
      721 tactggttac gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc
      781 gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg
      841 ccgttaggac gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc
      901 cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa accgctgttg
      961 ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg
     1021 agtctgacgc agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt
     1081 ggcgcgttac tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt
     1141 cctcagctct ggatcctggc ggtatttgtc gcgggtgtct ggctgatgcg cgctgctgga
     1201 tgtgtggtga atgattatgc tgaccgaaag tttgatggtc atgttaagcg cacggcgaac
     1261 cgaccacttc ccagcggcgc ggtaacagag aaagaggcgc gcgcactgtt tgtagtgctg
     1321 gtactgattt cgtttttact ggtactgacg ctgaatacga tgaccattct gttgtcgatt
     1381 gccgctctgg cgttggcgtg ggtgtacccg tttatgaagc ggtataccca tctcccgcaa
     1441 gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg cttttgccgc tgtgagtgag
     1501 tcggtgccat tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac
     1561 gacacgcagt atgcgatggt tgaccgcgat gatgatgtga agattggcat taaatccacg
     1621 gcaatcctgt tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg
     1681 gcactgatgg cgatcatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt
     1741 gtggtggctg gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa
     1801 gcctgcttta aagcatttat gaataataac tatgttggtc tggtactatt tttagggctg
     1861 gcaatgagtt actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt
     1921 ctgggttgcc tgatgcgcgg cgcttctcag gcctacacaa cacatcgcaa tttattgaat
     1981 ttgcagatta tggaaggccg gataaggcgt tttcgccgca tccggcaatt ctctctgatt
     2041 acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg
     2101 ccagcaactg ataaaccttc atcgtttctg ccggttcggc atcgccgcta tcgctgatat
     2161 acccttcatc acgcagtgtc agcaccagag aactgaacac cgccttgtcg aagaactccg
     2221 gcgcgttgat gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca
     2281 gcgtaccgcg gttgatcgac gggttggcac tcaacaacca gaaggtgatg gcataacgtt
     2341 gcagcgtttc gcgcgcgcct gcggcgagca gctgtagcgt gcgagaatgc accgggttga
     2401 tatgcaactc atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg
     2461 catcaataac gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca
     2521 ttgggtaaag cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg
     2581 tgacaattgc cgccatcagc gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt
     2641 aggtcatcag caccgcttgc tcgcgtggca gaatgatgat gtcgccgata gtgtctttct
     2701 cgacttcaaa cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag
     2761 gaacggtaga gtccgtggag taaggtacgt tgcgcatcag atccaggtag cagttgagtt
     2821 gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga tgccagtagc gcagtacagc
     2881 acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa
     2941 tattattgac cgtcggcgtt aaccaggccg gacgcaccgc ttcgatggga tcgatagatt
     3001 cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaagt
     3061 tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc
     3121 tctctttctc tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt
     3181 gctcataacc gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg
     3241 cctgaatggt catcgacagc gtaccggttt tcggatccag caaacgcccc gtgcgggaac
     3301 gaccgccttc cacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact
     3361 cacggaaaac ggtggaataa agtttattgc ctttaaaagt acggcgaata aagaacgccc
     3421 ccagacggcg gaaaatcggc ccggccggcc agaagttcag gttgatcccg gcggcgatat
     3481 gcggcggcac cagcccctgg tggtacagca cgtaagaaag tagcaggtag tccatgtgac
     3541 tgcggtggca aggcacatac accagctcat gaccgtcgtg ggccagctgg cgaacgcgct
     3601 cggcgttatg gacgttgatg ccctgataaa gtcggttcca ggtgaagccc agaatgcggt
     3661 cagtcaggcg gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg
     3721 cgttctgctg cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt
     3781 tggcaatggc gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg
     3841 ggcccacagc agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct
     3901 gagcgatagt tttatctgtg ccgtgttcat cggccatgcg gcgcagagaa actgacggcg
     3961 agaaacgcac aaaactgtcg cgaccgagcc acagtacggc gaaaaatttt tgtacgccgt
     4021 taagcatacg cagcggcggg tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca
     4081 tcaccgatac tggcaccatc tgcacatcca gatttgggtt gctgcggtgc aaatcaagat
     4141 agtcgtggaa cagcttaata gactcttctt tcggcgtgta ataggtgaac acacgcggcc
     4201 cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg
     4261 ggtcaggcaa gtcatgagcc agacactggg cgcgcaacgt cagcaaatct gctttcgagt
     4321 tgtacggtaa aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat
     4381 ctgccggaat agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt
     4441 aaattcgtgg ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc
     4501 ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg
     4561 ttacgcaaac ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa
     4621 aaggttcttt caatggccaa taataccact ggattcaccc gaattatcaa agctgctggc
     4681 tattcctgga aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc
     4741 gtagcggtat tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc aattacccgc
     4801 gtgctgctta tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc
     4861 gaagcagtgg ttgaccgaat tggctctgaa taccatgagc tttccggacg cgcaaaagat
     4921 atgggatccg ccgcggtgct gattgccatt atcgtcgccg tgattacctg gtgcatcctg
     4981 ttatggtcgc attttggata acccttccag atttcgataa atctctggtt tattgtgcag
     5041 tttatggttc caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc
     5101 agggggcgga atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga
     5161 tcacatcagc cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt
     5221 ccgttcccca aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga
     5281 aattgtttcc ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc
     5341 gctggtaggt cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca
     5401 ttatcaggtc gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg
     5461 gatgtcgatg aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca
     5521 ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg
     5581 cctgaaaaaa cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat
     5641 tgtcgttgac cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg
     5701 caacggcgac tggctgtaac atatctctga gaccgcgatg ccgccaggcg tcgcggtttg
     5761 tttttcatct ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc
     5821 actctggcat ctcgccttac ccatgatttt ctccaatatc accgttccgt tgctgggact
     5881 ggtcgatacg gcggtaattg gtcatcttga tagcccggtt tatttgggcg gcgtggcggt
     5941 tggtgcaacg gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac
     6001 cgggctgact gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt
     6061 gcaaccgttg ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacaccgat
     6121 tatcgatctg gcgctgcata ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg
     6181 ctttcttgaa atccgctggt taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg
     6241 ttggttactc ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat
     6301 cctcaacatt gtgctggatg tctggctggt gatggggctg catatgaacg tgcagggcgc
     6361 ggcgctggcg acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg
     6421 taaaatcctc aaactacgcg gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa
     6481 cttccgtcgc ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact
     6541 ctgtttcggc gcgatcaccg tacttggtgc gcgactgggg agtgacattg tcgctgttaa
     6601 cgcggttctg atgacgctac tcacctttac cgcctatgcg cttgatggtt ttgcctacgc
     6661 ggttgaagcg cactccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt
     6721 atggcgggca gcgtgccgcc agtcggggat tgtagcgtta ctgttttcgg tggtttattt
     6781 gctggcaggg gaacacatca ttgcgttgct gacgtcgtta acccagattc agcagctggc
     6841 tgaccgctat cttatctggc aggtgatttt gccgttggtt ggcgtctggt gttatctgct
     6901 ggacggcatg tttataggcg caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc
     6961 cgccgcaggt tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg
     7021 gctggcatta accgtctttc tggcgttgcg cgggctttct ctggcggcta tctggcggcg
     7081 tcactggcgc aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata
     7141 atcctaagcc aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact
     7201 ctcattcaca acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact
     7261 ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga
     7321 cgatcattga aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc
     7381 agaaagatca ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt
     7441 aattaatccc tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt
     7501 tcacctgcac agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca
     7561 cgcatgccgc acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt
     7621 ttgccgccag cgtatgcata atgtcttcca cgccttctgc acactcttct ttcaccgcgc
     7681 cgcagcgatc gcaaataaac atggctgacg tatgggtggg ctggtcgaac agatgacaga
     7741 gcacataact gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca
     7801 gcgcgcgata aaccgttggc ggcttggctt gcggttcagc ttcgcgcagt aaatcaagca
     7861 gatcataagc gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct
     7921 gtggggtcag gcgcacatta cgctgcgcgc agattttttc agcctgcgct aataactcct
     7981 gcgttgtggt cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac
     8041 catgttccag gaaaaacacc gagaaccctt attgttgccg taatgttgat tttctgtttt
     8101 gtaggtaagg tgttgtgttg ccttgtcgta cc
//
LOCUS       DAZFRJ010000185         8101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000185 DAZFRJ010000000
VERSION     DAZFRJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.187"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            551..1834
                     /gene="ydfJ"
                     /locus_tag="ACHKUN_003888"
                     /pseudo
     CDS             551..1834
                     /gene="ydfJ"
                     /locus_tag="ACHKUN_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1923..3383
                     /locus_tag="ACHKUN_003889"
     CDS             1923..3383
                     /locus_tag="ACHKUN_003889"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6662062.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3419..3622)
                     /gene="ydfZ"
                     /locus_tag="ACHKUN_003890"
     CDS             complement(3419..3622)
                     /gene="ydfZ"
                     /locus_tag="ACHKUN_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO6662063.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3800..4486)
                     /gene="rspR"
                     /locus_tag="ACHKUN_003891"
                     /gene_synonym="ydfH"
     CDS             complement(3800..4486)
                     /gene="rspR"
                     /locus_tag="ACHKUN_003891"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO6662064.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4575..5321)
                     /gene="ydfG"
                     /locus_tag="ACHKUN_003892"
     CDS             complement(4575..5321)
                     /gene="ydfG"
                     /locus_tag="ACHKUN_003892"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO6662065.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5458..7503
                     /gene="dcp"
                     /locus_tag="ACHKUN_003893"
     CDS             5458..7503
                     /gene="dcp"
                     /locus_tag="ACHKUN_003893"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO6662066.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7548..8066)
                     /locus_tag="ACHKUN_003894"
     CDS             complement(7548..8066)
                     /locus_tag="ACHKUN_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO6662067.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2045 a         1847 c         2066 g         2143 t
ORIGIN      
        1 ttgggtgttc ttaaagttcg tagataaaca ttgtcgtttc aggtatacag gaatgctaac
       61 aggtggcagc aaaaatcagg cggtttatgg cgcaagctga agcggcaact gcaaactatc
      121 ttatgcagag actctacacg gattgggttt aaaagtatac atagataaca gtttttatct
      181 gaaaaagaaa aatatcaagg tgatatagcc tatatgcctt tgatgtggag gaatgaatgt
      241 gatgggagtg atgtatctga atagttgaaa aaccgcagac acaccttatg caagaacgtg
      301 ctgcggttgg ctggtaaatt tttcgatagt gcgagtattg aatgatttcc agccgttatt
      361 gattttacgc gtaaatccat gaaaaaacta cttatctgtt ggggagtttt ttggggcata
      421 tatgggacag aaataggccc cagatagaca ctgagatcga acttaggatg cttttaaaaa
      481 aatgcaacta tctgaaaaaa cctagaaaac gccaaggaaa ccacaggatg ggaaaaaaca
      541 cctgtgaatt atggatttcc agttatattc gctcggcgca gcgttagtgt ttcatgaaat
      601 attttttcct gaatcatcaa cggcaatggc gttaattttg gcaatgggaa cctacggtgc
      661 aggttatgtg gcgcgtattg tcggagcatt tattttcggc aaaatgggcg acagaatagg
      721 gcgtaaaaaa gtgctcttta ttaccatcac catgatgggg atctgtacca ccttaattgg
      781 tgtgttaccg acctatgcac agattggtgt ttttgccccc atcttgctgg tgacgctgcg
      841 tattattcag gggttgggtg caggtgcgga aatttccggt gccggtacga tgctggcgga
      901 atatgcgcca aaaggtaagc gcggaattat ctcctcattt gtagctatgg gaactaactg
      961 cggaaccttg agcgcaacgg caatctgggc ctttatgttc ttcattctca gtaaagagga
     1021 actgcttgcg tggggatggc gtataccgtt cctggcgagc gttgtcgtga tggtctttgc
     1081 tatctggttg cgtatgaatc tgaaagaaag cccggtcttt gagaaggtta acgacagcaa
     1141 ccaacccaca gcaaaacctg cacctgctgg tagcatgttc cagagcaaat ccttctggct
     1201 ggcaacaggg ctgcgttttg gtcaggcggg taactccggg ttaattcaga ctttccttgc
     1261 aggctattta gtgcagacgt tattgtttaa caaagcaatt ccaacagatg cattgatgat
     1321 tagttcgatt ctcggcttta tgaccattcc gttccttggt tggttatccg ataaaattgg
     1381 tcgccggatc ccgtatatta ttatgaatac ctccgcgatt gtgctggcat ggccaatgct
     1441 ttctatcatt gtagataaaa gctatgcccc gagcaccatt atggttgcac tgattgtgat
     1501 tcataactgt gcggtgctgg gattatttgc tctggaaaac attaccatgg cagaaatgtt
     1561 cggctgtaaa aaccgcttta cccggatggc tatttctaaa gaaattggtg gtcttatcgc
     1621 ttccggtttt ggtcctatcc tggcgggtat tttctgcacc atgacggaat cctggtatcc
     1681 gatcgccatt atgatcatgg catattcagt aattggttta atctctgcgc tgaaaatgcc
     1741 agaggtgaaa gaccgtgatt taagtgcgct ggaagacgct gcggaagatc aaccgcgtgt
     1801 tgtaagagct gcgcaacctt ccagaagtct gtaaatcctc aatcccttct cttaccggag
     1861 aggggatttt tattcatata acaaaacata tagcttgcca tatttatatt taaggaattg
     1921 ttatgggaaa taatttgtta tcagcaaaag cgacactccc tgtttatgat cgtaataacc
     1981 tggccccaag aattattcat ttaggctttg gtgcatttca ccgtgcgcat cagggcgtgt
     2041 atgccgatat tcttgctaca gaacatttca gtgactgggg atattatgag gtcaacttaa
     2101 tcggcggcga acagcaaatt gccgatttac aacagcaaga taatctttat accgttgcgg
     2161 aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt cgttaaaaaa gccttgcacg
     2221 tacagatgga tggcttagaa accgtgttgg ctgcgatgtg tgaaccgcaa atcgcgattg
     2281 tctctctgac aatcaccgaa aaagggtatt tccactctcc ggcgatcgga cagttaatgc
     2341 tcgatcaccc gatggtcgct gccgacgtgc aaaatcccca ccagccgaaa acagcaacag
     2401 gagtgattgt tgaggcgctg gcccgccgta aagcggcagg acttcccgca tttaccgtca
     2461 tgtcatgtga caacatgcca gaaaacggtc atgttatgcg tgacgttgtc acttcatacg
     2521 cgaaagctgt tgatgtaaaa ctggcacaat ggatcgaaga taacgtgact ttcccatcaa
     2581 caatggtgga ccgtattgtg cccgcagtga cagaggatac gctggcgaaa atcgaacaac
     2641 ttaccggtgt gggcgatcct gcgggcgttg cctgtgaacc tttccgccag tgggtaatag
     2701 aagataactt tgttgccgga cgtccagaat gggaaaaagc gggagccgaa ctggttagtg
     2761 atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa cggcagtcat tcattcctgg
     2821 cgtatttggg ttatcttgct ggatatcagc acattaatga ctgcatggaa gatgaacatt
     2881 atcgttatgc ggcgtatggc ttgatgttgc aggaacaagc gccgacgctg aaagtgcagg
     2941 gcgttgattt gcaagattac gctaaccgat taattgcacg ctatagcaac ccggcgttac
     3001 gtcatcgaac ctggcagatt gcgatggatg gtagccagaa attgccacag cggatgttgg
     3061 attctgttcg ctggcatctg gcgcatgaca gcaagttcga tctgctggcg ctgggcgtcg
     3121 cgggttggat gcgttatgtc ggtggtgttg atgaacaggg aaatctgata gaaatcagtg
     3181 acccactgtt acctgttatt cagaaggctg tacaaagtag tgccgaaggg aaagcgcgcg
     3241 tccagtcatt gctggcgatt aaggcaatct ttggtgatga tttgccagac aatagcttgt
     3301 ttactgcaaa agtgacggaa gcgtacttgt ctttattagc gcatggcgcg aaagcgaccg
     3361 tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca tcggcggcat tcacacactt
     3421 agttcatgcc gagacgaatc aggtccagag gtgccagttt ctcttcacaa ccttcaacaa
     3481 ctacggtttt tccgcggcgg atttgctcag cggtcagacc ttcagtatca atcgacagga
     3541 tcttgccagt gtgaccggtg ccgctaacca taacacggct gccagtggtg attgcgttac
     3601 ggttacgatc gtaggtcgtc ataggtgttt tctcctttct gatttacagt cgaagcccac
     3661 gtaacgggcg caatataaat actcctgtcg agtgaaatat tttttgtttt tgatcaagct
     3721 cacatctttt ttgatgcatg catgagccgt aaggcggggg gaaaagcctc tcatcccgga
     3781 tgggatgaga gtaaatgaat tactcttcgc taaaccagtc gctgttttcc tggcggatct
     3841 ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat tgcacgttct accgcatcgc
     3901 catcacgttt ttgcagggca gagaaaatat caagatgctg gcgtaacagc atttctggtg
     3961 gagaaacatg gtcgaaactc atatagcgca cgcgatcaac ggtcgctttc aggttctcaa
     4021 tggtatccca cgccagttga cagtcggcaa tctgcgtcag gagttgatgg aagttgtcat
     4081 caagttcaaa aaaatcatcc agttgcttgc gctcaatggc aatgcgttgc tggtgaagat
     4141 tttgttccag ttggtagcac tggctttcgg taatcatgct cgccgcccga cgcgccaccg
     4201 cgcactcaat ggcctgacgg ataaaactgc cgtttcgcac ctgggccatg gaaattttgt
     4261 tgacgtagct gccacgctgc ggacgaattt gaatcaggcc gttttccgcc agtttaataa
     4321 aggcttcacg aaccggctgg cgtgacacat tgaaacgaac agaaacttct ttttccgaca
     4381 acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg acgaagaata cgataaatct
     4441 gctgattgac aggctgtgtg ggattaagtt gcgtttcgac ggtcattcgt tcttgcttgt
     4501 gagtgagtta actgcatgag tctaccactt ttttgtgaca agcaataacc cggcagttac
     4561 gccgggtata aaaattactg acggtggaca ttcagtccgg catagctttg ggtaaccggc
     4621 atcatttcca gggtattgat attgacgtga gcaggcagcg ttgacaccca ccagacggct
     4681 tcgctgacat cttctggcgt caatgcaacg gtattttgat aggttttttc cgctttaccg
     4741 tcatcgcctt taaagcggac attggaaaac tcggtgccac ccaccagacc cggttcgatg
     4801 tcggtgacgc gcaccgccgt accatgcagg tccgtacgca gattcaggct aaactgacga
     4861 acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat aaggccagct acctgccgtt
     4921 gagccaatgt taataatatg accatgatta cgttcaacca tacccggtaa gacggcgcgc
     4981 gtcatatata ccaggccttt gttgttggta tcaatcatcg tttcccagtc ttcaacgctg
     5041 gctttatgcg caggctccat gcccaacgcc aggccggcat tatttaccag gatatcaata
     5101 ttgcaccact cggcagggag cgatgccagc atctcttcaa tagcggcgcg gttgcgaacg
     5161 tccagttggg cgatatacag attatctccc agttcgtctt ttaactcctg caaccgctcc
     5221 tggcgacggc cagtggcgat aactttatgc ccttgttgaa taaaacgacg agtaatgcat
     5281 tcaccaaaac ctgccgttgc tccagttact aaaacgatca tctcactgtt cctcaacgct
     5341 tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg gtaatactgg tttgcaagat
     5401 tgcactccgt tatcagtaag cctacgctat ggaacacctc gcgttcagga gaagaaaatg
     5461 acaacaatga atcctttcct tgtgcaaagc acactgccgt atctggctcc tcattttgat
     5521 caaattgcca atcatcacta tcgcccggca ttcgatgagg gaatgcagca aaagcgggca
     5581 gaaattgctg ccatcgcgct taacccgcaa acgcctgatt tcaacaatac tattctggca
     5641 ctggaacaaa gcggagaatt acttacccgc gttaccagcg tcttttttgc gatgactgcg
     5701 gcgcatacca atgatgaatt acagcgtctt gatgaacagt tttccgctga actggcggaa
     5761 ctggctaatg atatctatct gaacggtgaa ttattcgcgc gggtagatgc tgtctggcag
     5821 cgccgtgaat ccctggggct tgatagtgaa tccatccgcc tggtggaggt gattcatcaa
     5881 cgttttgtcc ttgccggagc caaacttgcg caagctgata aagcaaaatt aaaagtactg
     5941 aatacagaag ctgcgacact gaccagtcag tttaaccagc ggttactggc agcaaataaa
     6001 tccggcggtc tggttgtgaa cgatatcgcg cagctggcag gaatgagtga gcaagagatt
     6061 gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata acaaatggct gattccgctg
     6121 ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg atcgtgcgac gcgtgaaaaa
     6181 ctgtttattg cgggctggac gcgagcggaa aaaaatgatg gcaatgatac ccgcgctatc
     6241 attcaacgtc tggtggagat ccgtgcacaa caggcgacat tacttggttt tcctcattat
     6301 gccgcatgga aaatcgccga tcagatggca aaaacacctg aagcagcgct caactttatg
     6361 cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg aattagcctc catacaggcg
     6421 gttattgata agcagcaggg cgggtttagc gcgcagccgt gggactgggc attttatgcc
     6481 gaacaggtac ggcgggagaa atttgatctt gatgaggcgc agctcaagcc atattttgaa
     6541 ttaaacacgg tgttgaatga aggtgtattc tggaccgcga atcagctctt cggtattaag
     6601 tttgtcgaac gttttgatat tcctgtctac catcctgacg ttcgtgtgtg ggaaattttt
     6661 gatcataatg gcgtgggact ggcgttattt tacggtgatt tcttcgcccg tgattcaaaa
     6721 agcggcggtg catggatggg caattttgtt gagcaatcaa cgcttaatga aacgcattcg
     6781 gtaatttata acgtttgcaa ttatcagaaa cccgctgccg gtgagcctgc gttgttactc
     6841 tgggatgatg tcataacctt attccatgaa tttggtcata cgctgcacgg cctttttgcc
     6901 cgccagcgtt atgccacgct ttccggcacc aacacgccgc gtgattttgt cgaatttccg
     6961 tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat tcgctcgcta cgcccggcat
     7021 tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga aaatgcgtaa tgccagcctg
     7081 ttcaacaaag ggtatgagat gagcgaactg cttagcgccg cacttctcga tatgcgctgg
     7141 cattgcctgg aagaaaacga agcaatgcag gatgtcgatg attttgaatt gcgggcgctg
     7201 gtggcggaaa atatggatct tcctgctata ccgccacgct atcgcagcag ttatttcgcc
     7261 catatttttg gtggcggata tgccgcaggt tattacgctt atctgtggac acaaatgttg
     7321 gccgatgatg gttatcagtg gtttgttgag cagggcggat taacgcgtga aaatgggctg
     7381 cgttttcgcg aggcgatcct ttccagaggt aacagcgaag atctggaacg cctgtatcga
     7441 caatggcgcg gtaaggctcc tcagattatg ccgatgctgc aacatcgtgg cttgaacata
     7501 taaacaccac caatggctgt cctggagata tcgccaggcc agcctggtta tagcagtaat
     7561 tgcagcagct gggcgagggc aaacctcacc gctaaagcta ataccgtttc gcagtcgcca
     7621 gcaaaatgca taaccgcagt ataggtctgg cctttaatat gccacgcgaa ccagacggta
     7681 cccgctggcg taccatcttc accgccctcc ggtccgccgt agccggtaat ggcaatactt
     7741 acatcagcat ccgcacgctc tatggctccg gttgccattt ctgctgccac tttctcactc
     7801 accgcagaat atcgttcaag agattgctgg cttacgctga ggattttcat ctttgcctga
     7861 tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg tatcttcagc tgcacacagg
     7921 gcgctagcca acttaccgcc ggtgcatgat tcggcggtag ttaagcgtaa ttgatccgcg
     7981 ataagtcttt gactcaacgc ggatgtcagg ttttcaatag tgtcggtatc tgcgagttgt
     8041 acaattttat ctttattaat gttcactgtt ttttgtccac gctattatat gtaaaggtga
     8101 t
//
LOCUS       DAZFRJ010000186         8069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000186 DAZFRJ010000000
VERSION     DAZFRJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.188"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..869
                     /locus_tag="ACHKUN_003895"
     CDS             39..869
                     /locus_tag="ACHKUN_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6662068.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            901..1836
                     /locus_tag="ACHKUN_003896"
     CDS             901..1836
                     /locus_tag="ACHKUN_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6662069.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1929..2171
                     /gene="yhdT"
                     /locus_tag="ACHKUN_003897"
     CDS             1929..2171
                     /gene="yhdT"
                     /locus_tag="ACHKUN_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO6662070.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2161..3612
                     /gene="panF"
                     /locus_tag="ACHKUN_003898"
     CDS             2161..3612
                     /gene="panF"
                     /locus_tag="ACHKUN_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO6662071.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3624..4505
                     /gene="prmA"
                     /locus_tag="ACHKUN_003899"
     CDS             3624..4505
                     /gene="prmA"
                     /locus_tag="ACHKUN_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO6662072.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4834..5799
                     /gene="dusB"
                     /locus_tag="ACHKUN_003900"
     CDS             4834..5799
                     /gene="dusB"
                     /locus_tag="ACHKUN_003900"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO6662073.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5825..6121
                     /gene="fis"
                     /locus_tag="ACHKUN_003901"
     CDS             5825..6121
                     /gene="fis"
                     /locus_tag="ACHKUN_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO6662074.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6207..7091
                     /gene="yhdJ"
                     /locus_tag="ACHKUN_003902"
     CDS             6207..7091
                     /gene="yhdJ"
                     /locus_tag="ACHKUN_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6662075.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7175..7354
                     /gene="yhdU"
                     /locus_tag="ACHKUN_003903"
     CDS             7175..7354
                     /gene="yhdU"
                     /locus_tag="ACHKUN_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO6662076.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7357..8019)
                     /gene="acrS"
                     /locus_tag="ACHKUN_003904"
     CDS             complement(7357..8019)
                     /gene="acrS"
                     /locus_tag="ACHKUN_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO6662077.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         1987 a         1959 c         2038 g         2085 t
ORIGIN      
        1 aaaatacaag gggctatagt gttacgcatt aaaggccaat gaagagtgtt aacgtgccga
       61 gaaaatcgtc taacctactg aaacgcagat taaaaattgc agaaattgtt gccgcccaag
      121 gtgaaatcaa agttgatgac ttatctgcgc aacttggtgt ctctggcgtg acgatccgcg
      181 gcgatctcag ttatctggag caacagggat atttaaagcg atcatttggc ggtgctattg
      241 cgacaccact ctcctctgaa ccagaagcga tacaaccgat cgcgccattg tctctggcac
      301 aacaaatgga aatagcacga cattgcgcac ggttaattcg cgatcgtgac acgctatttc
      361 ttggacatgg gacaatctgc cgtaagatca ttccattgct cagcgaagtg aaaaaactgc
      421 gtctgatcac caacgatccc gaacatgcgc tgctggcaaa tcagtttatt gatggcgaaa
      481 taatactggc tggcagtgaa atgctgcgcc ccgacaccgc actggccgga aaaccgcttg
      541 aacaggcact tcagcatttc accattacac atagcattct ggaaatcagc catctggatg
      601 ctgatggtac tttaaatatc aacgttccca gactggccgc tgcctggcag ctctgtttcg
      661 atcatgccca gaataagacg gttatcgttg ccgccgatcc tgcgccatca acggctgcta
      721 tcgggcattt gcatcaggca gaaaatgtga tcgtcagccg cagcattaac gaacaatatc
      781 agcaacaatt gcagtcggct gatttcgtta tcctttatac cagtaatgag tgctttacct
      841 ggtccaatcg cgaaaggctt caggaatgag aggggttttg tgacaataac tatctgcgct
      901 atgggcgaat tgctggccga gtttttgtcc cgcaacccac atcaaaaatt cactcagcct
      961 ggggagttta tcgggccatt tcccagcggt gcgccagcaa tttttgctgc tcaggtggca
     1021 aaactgtccc atcgggccat cttcttcgga tgtgttggta atgatgattt tggccgactc
     1081 attatagagc gcctccgtca tgaaggtgtc attaccgatg ggatccatgt tatgaacaat
     1141 gccgtcacag gtacggcgtt cgtgagttat caaaatcccc agcagcgcga tttcgtcttt
     1201 aatatcccta acagcgcctg cggcttgttt actgctgagc acattgataa ggatctgcta
     1261 aaacagtgta accatctcca tattgtgggc tcatcgttgt tctcatttcg catgatcgat
     1321 gtcatgcgta aagcaataac gacgatcaaa tcggctggcg gcaccgtttc tttcgatccc
     1381 aatattcgca aagagatgct gagcattcct gaaatggcgc aggctctcga ttatttgatt
     1441 gaatatacgg atattttcat ccccagcgaa agcgaactcc ctttcttcgc gcgtcacaaa
     1501 aatctgtcag aagaacagat tgttagcgat cttctccacg gcggcgtaaa acatgtggcg
     1561 ataaaacgcg cccagcgtgg ggccagctat tacaagctta aaaacggtac attacacgcc
     1621 cagcatgtta caggccacga tatcgaaatt atcgatccaa cgggtgcagg cgactgcttt
     1681 ggcgcaacgt ttatcactct tttcttatcc ggtttcccgg cacataaggc actgcaatat
     1741 gcaaatgcca gcggcgcgct cgccgtaatg cggcaaggtc cgatggaagg gatatcctca
     1801 ctggctgaca ttgaagactt tttgcagcag cactgaacgg caattttctg tcagattatt
     1861 ttccctgcct gaagagttgc cgtagaattg cccgtttctc gtatcccccc atgaggagga
     1921 acccttccgt ggacactcgt tttgttcagg cccataaaga ggcgcgctgg gcgctggggc
     1981 tgaccctttt gtatctggca gtttggttag tagccgctta cttacctggc gttgcccccg
     2041 gttttaccgg ctttccgcgc tggtttgaga tggcctgcat cctgacgccg ctgctgttta
     2101 ttggactgtg ctgggcgatg gtgaaattta tctatcgcga tatcccactg gaggatgacg
     2161 atgcagcttg aagtaattct accgctggtc gcctatctgg tggtggtgtt cggtatctcg
     2221 gtttatgcga tgcgtaaacg gagcaccggc accttcctta atgagtattt cctcggcagc
     2281 cgctctatgg gcggtattgt gctggcgatg acgctcaccg cgacctatat cagtgccagt
     2341 tcgtttatcg gcgggccagg agctgcttat aaatacgggc tgggctgggt attgctggcg
     2401 atgattcagc ttcctgcagt ctggctttca ctcggtattc tcggcaagaa gtttgcgatt
     2461 cttgcgcgcc gctacaatgc agtgacgctg aacgatatgc tgtttgcccg ctaccagagt
     2521 cgtcttctgg tgtggctggc gagtttgagt ttgctggttg cgttcgttgg tgcgatgacc
     2581 gtgcagttta tcggcggtgc gcgcctgctg gaaaccgcgg cgggtattcc ttatgaaacc
     2641 gggctgctga tttttggtat cagcattgcg ttatataccg cctttggtgg ctttcgcgcc
     2701 agcgtgctga acgacaccat gcaagggctt gtgatgctga ttggcaccgt tgtgctgctt
     2761 attggcgtgg tacatgccgc tggcggctta agcaacgcag tacagacctt gcaaaccatc
     2821 gatccgcaac tggttacgcc acaaggcgct gacgatattc tgtcgcctgc ctttatgacg
     2881 tcgttctggg tactggtgtg ctttggcgtg attggcctgc cgcatactgc ggtgcgctgt
     2941 atctcttata aagacagcaa agcagtacac cgggggatca tcatcggtac gattgtagtc
     3001 gcaatcctga tgttcggtat gcacctggcc ggagcgttag gtcgtgcagt gatccccgat
     3061 ctcaccgtac cggatctggt gatcccgacg ttaatggtaa aagtgctgcc accgtttgct
     3121 gccggaatct tcctggctgc acctatggct gcgatcatgt cgacgatcaa tgcccagttg
     3181 ctgcaaagtt ccgctacgat cattaaagat ctctatctga atatccgtcc ggatcaaatg
     3241 caaaacgaga cgcgtctgaa gcggatgtcg gcggtaatta cgttagttct cggcgcgttg
     3301 ctgctgcttg ccgcctggaa gccgccagaa atgatcatct ggctgaattt gctggccttc
     3361 ggtgggctgg aagccgtttt cctgtggccg ctggtgctgg gtctttactg ggaacgcgcc
     3421 aacgccaaag gcgcgctaag tgcgatgatc gttggcggcg tgctgtatgc cgtactcgcg
     3481 acgctgaata ttcagtacct gggcttccac cctattgtgc cctcgttact actaagtttg
     3541 ctggctttcc tggtcggaaa ccgtttcggt acatccgtcc cgcaagctac cgttttgact
     3601 actgataaat aaagagtttt gccatgcctt ggatccaact gaaactgaac accaccggcg
     3661 cgaacgcgga agatcttagc gatgcgctga tggaagcggg tgccgtttct atcacttttc
     3721 aggataccca cgatacgcca gtatttgagc cgctgccggg cgaaacgcgc ctgtggggcg
     3781 acaccgatgt gattggtctg ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc
     3841 tggaaaacca tccgctgctc ggcgcaggct tcgcgcataa aatcgaacaa ctagaagata
     3901 aagactggga gcgcgaatgg atggataatt tccacccgat gcgctttggt gaacgactgt
     3961 ggatctgccc tagctggcgt gatgtgccgg acgaaaacgc cgtcaacgtg atgttagatc
     4021 cagggctggc gtttggtacg ggtacccatc caaccacctc tctgtgcctg caatggctcg
     4081 acagcctcga tttaaccggt aaaacagtca tcgactttgg ctgtggttcc ggcattctgg
     4141 cgatcgcggc gctgaaactg ggtgcagcaa aagccattgg tattgatatc gatccgcagg
     4201 cgattcaggc cagccgcgac aacgccgaac gtaatggcgt ttctgaccgt ctggaactct
     4261 acttaccgaa agatcagcca gaagaaatga aagccgacgt ggtggtcgct aacatccttg
     4321 caggcccatt acgtgaactg gcaccgttaa tcagcgtcct gccggtttca ggcggtttgc
     4381 tgggcctttc cggtattctg gcaagccagg cagagagcgt ttgtgaagct tatgccgata
     4441 gcttcgcact ggacccggtc gtggaaaaag aagagtggtg ccgtattacc ggtcgtaaga
     4501 attaaccttc gcatcgccgt agggtgacgc ggggcgaagt gcgagcaagc tcacaaaagg
     4561 cacgtaaatt tgccggttat ttacgcaaat ttgcgtgcca aaattttcat tcataaagaa
     4621 aaattgagaa cttactcaaa tttctttgag tgtaaatttt agtcactatt ttctaatatg
     4681 atgattttta tgagtaatta tcgcaccacg ctcattttaa atgcaattct ttgatccatc
     4741 tcagaggatt ggtcaaagtt tggcctttca tctcgtgcaa aaaatgcgta atatacgccg
     4801 ccttgcagtc acagtatggt catttcttaa ctcatgcgca tcggacaata tcagctcaga
     4861 aatcgcctga tcgcagcgcc catggctggc attacagaca gaccttttcg gacgttgtgc
     4921 tacgagatgg gagccggatt gacagtatcc gagatgatgt cttctaaccc acaggtttgg
     4981 gaaagcgaca aatctcgttt acggatggtg cacattgatg aacccggtat tcgcaccgtg
     5041 caaattgctg gtagcgatcc gaaagaaatg gcagatgcag cacgtattaa cgtggaaagc
     5101 ggtgcccaga ttattgatat caatatgggt tgcccggcta aaaaagtgaa tcgcaagctc
     5161 gcaggttcag ccctcttgca gtacccggat gtcgttaaat cgatccttac cgaggtcgtc
     5221 aatgcagtgg acgttcctgt taccctgaag attcgcaccg gctgggcacc ggaacaccgt
     5281 aactgcgaag agattgccca actggctgaa gactgtggca ttcaggctct gaccattcat
     5341 ggccgtacac gcgcctgttt gttcaatgga gaagctgagt acgacagtat tcgggcagtt
     5401 aagcagaaag tttccattcc ggttatcgcg aatggcgaca ttactgaccc gcttaaagcc
     5461 agagctgtgc tcgactatac aggggcggat gccctgatga taggccgcgc agctcaggga
     5521 agaccctgga tctttcggga aatccagcat tatctggaca ctggggagtt gctgcccccg
     5581 ctgcctttgg cagaggttaa gcgcttgctt tgcgcgcacg ttcgggaact gcatgacttt
     5641 tatggtccgg caaaagggta ccgaattgca cgtaaacacg tttcctggta tctccaggaa
     5701 cacgctccaa atgaccagtt tcggcgcaca ttcaacgcca ttgaggatgc cagcgaacag
     5761 ctggaggcgt tggaggcata cttcgaaaat tttgcgtaaa cagaaataaa gagctgacag
     5821 aactatgttc gaacaacgcg taaattctga cgtactgacc gtttctaccg ttaactctca
     5881 ggatcaggta acccaaaaac ccctgcgtga ctcggttaaa caggcactga agaactattt
     5941 tgctcaactg aatggtcagg atgtgaatga cctctatgag ctggtactgg ctgaagtaga
     6001 acagcccctg ttggacatgg tgatgcaata cacccgtggt aaccagaccc gtgctgcgct
     6061 gatgatgggc atcaaccgtg gtacgctgcg taaaaaattg aaaaaatacg gcatgaacta
     6121 attcaggtta gctaaatgct tgattaaaaa ggcgctactc ggcatgggga agcgcctttt
     6181 ttataggtgt cacaaaggga gtgaccatga gaacaggatg tgaaccgacc cggtttggta
     6241 atgaagctaa gaccattatt cacggtgatg cgcttgccga acttaaaaag atccccgccg
     6301 aaagtgtcga tctgatcttt gccgacccac catataacat cggtaaaaat tttgatggtc
     6361 tgatcgaagc ctggaaagaa gatctgttta tcgactggct gtttgaagtg attgcagagt
     6421 gccaccgcgt tctgaaaaag cagggcagca tgtacatcat gaacagcacg gaaaacatgc
     6481 cctttatcga tctccagtgc cgcaagcttt ttaccatcaa aagtcgcatc gtctggtcat
     6541 atgacagttc tggagtacag gcgaaaaaac actacggctc catgtacgaa cccatcctga
     6601 tgatggtgaa agacgcaaag aactacacat tcaacggtga tgctattctg gtagaagcca
     6661 aaaccggatc gcagcgcgcg ttgatcgatt atcgcaaaaa tcctccacag ccatacaatc
     6721 atcaaaaagt accgggtaac gtctgggatt ttccgcgcgt gcgttatttg atggatgaat
     6781 atgagaacca cccgacgcaa aaaccggaag ccttactgaa acgcattatt ctcgcctctt
     6841 ccaacccagg cgatatcgtt ctcgacccgt ttgccggtag ctttactacc ggtgccgtag
     6901 ccgtctccag cgggcgaaaa ttcattggta ttgagatcaa cagcgagtac atcaaaatgg
     6961 ggcttcgacg gctggatgtc gcgtcgcatt actctgcgga agaactggcg aaagtgaaaa
     7021 aaagaaagac cggcaacctg tcaaaacgaa gccggttaag cgaagttgac cccgatctca
     7081 ttgcaaagta aaggaagtgt aagcctggtt ttcagattat tcatttcgtg tatatttcct
     7141 gccagacttg gttaaacata cacaggctca ggtaatgatt cgcaagtatt ggtggctcgt
     7201 cgttttcgct gtcttcgttt ttctgtttga tactttactg atgcagtgga ttgaactgct
     7261 ggcaacagaa acagacaaat gccgcaatat gaactcagtt aatccactaa aactggttaa
     7321 ctgtgacgaa ctgaattttc aggacagaat gtgaatttac atgacactta attcattcgt
     7381 ttgatgaatt aatttcgtta tgttttcatc tggcatgaac attcttaata cgttatcgac
     7441 cagagcgggg gcttgtttat aaagatcata acccgccata ttcattaacc agttttgaac
     7501 aattccgctg aaggcaccat taataataat catcacaaca tctaaatcga ggttatttgc
     7561 tacacaacct tgttgctgac acgcctgcaa tacttcgcgg agagtctgcg gattgaagcc
     7621 catcttttcg cgtatcactc cctcggccag catctcatca ttaaattcac atttgtgata
     7681 taagattttc agcaacgcct gctggcgggg aattttggca atatattgca agccgacaat
     7741 caatttttca cgcaattgtt gaaacgggtc atgctctaat ccagccgtca agtgttcctg
     7801 gattaactcc cgcaatgaag gctgttgcaa ccacatctca ttaaacagtt gagtcttgtt
     7861 ttcgaagtgc cagtagatag cgccacgcgt aacgttagca gcgtcggcaa tgtcgttgag
     7921 tgtcgtcttg cttacgccat gctgcgcaaa ctgggcgatg gcagtttcaa tcagttcttg
     7981 ccgggtcttc agagcttcgg ctttggttct ttttgccatg attaattatt caggaaataa
     8041 atatattcga cacagagtga gaaaatagc
//
LOCUS       DAZFRJ010000187         7917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000187 DAZFRJ010000000
VERSION     DAZFRJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.189"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..855
                     /locus_tag="ACHKUN_003905"
     CDS             <1..855
                     /locus_tag="ACHKUN_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFO6662078.1"
                     /translation="SEITLVSNPYYKYGDSTTNNETRLLSDSIADVLSYSIGCMMGRY
                     SLDREGLVYAHEGNKGFAELAAEGAYKTFPADNDGILPLMDDEWFEDDVTSRVKEFVR
                     TVWGEEHLQENLEFIAESLCLYAIKPKKGESALETIRRYLSTQFWKDHMKMYKKRPIY
                     WLFSSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANIDRLNDQLDEASGGE
                     ATRLKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVNYGKFGDLLADVKAI
                     TGNAPEAI"
     gene            1032..3629
                     /gene="pglZ"
                     /locus_tag="ACHKUN_003906"
     CDS             1032..3629
                     /gene="pglZ"
                     /locus_tag="ACHKUN_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFO6662079.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLENVTLI
                     NMTHASQLAVKKRIEMDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDETSLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKRHGLDPVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADRQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQAALRPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEMVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVADTAGVSDNSEFLIPKGIQRFHFSGGAHFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNSTMEKRVREVTLKLIGASFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            3640..5724
                     /gene="brxL"
                     /locus_tag="ACHKUN_003907"
     CDS             3640..5724
                     /gene="brxL"
                     /locus_tag="ACHKUN_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFO6662080.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPTAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            complement(5989..6414)
                     /locus_tag="ACHKUN_003908"
     CDS             complement(5989..6414)
                     /locus_tag="ACHKUN_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6662081.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRAVCTKYVYSYQPDLFQDCGSAST"
     gene            complement(6411..6794)
                     /locus_tag="ACHKUN_003909"
     CDS             complement(6411..6794)
                     /locus_tag="ACHKUN_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6662082.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(7166..7765)
                     /locus_tag="ACHKUN_003910"
     CDS             complement(7166..7765)
                     /locus_tag="ACHKUN_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6662083.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT         2038 a         1992 c         2024 g         1863 t
ORIGIN      
        1 tcagaaataa ctttagtcag caacccatac tacaagtacg gtgattcaac gactaataat
       61 gaaactcgtt tgttatcgga ttcaatagca gatgtattaa gttattccat cggctgcatg
      121 atgggtcgct actccctcga tcgcgaaggc ctcgtctacg cccatgaagg caacaaaggc
      181 tttgccgaac ttgccgctga aggtgcgtac aaaaccttcc cggcagacaa tgacggcatc
      241 ctgccgctga tggatgacga gtggtttgag gacgacgtca cctctcgcgt caaagagttt
      301 gtccgcaccg tctggggcga agagcacctg caggaaaacc tcgaatttat cgccgaaagt
      361 ctctgtttat acgcgatcaa gccgaaaaaa ggcgaatctg cgctggagac cattcgtcgc
      421 tatctttcca cacagttctg gaaagatcat atgaagatgt ataaaaagcg cccaatctac
      481 tggctattca gctccggtaa agagaaagcc ttcgagtgcc tggtgtatct gcatcgctat
      541 aacgacgcca cgctgtcgag aatgcgtacc gaatatgtgg tgccgctgct ggcacgttat
      601 caggccaata tcgatcgcct gaacgatcaa cttgatgaag cttctggcgg tgaagccaca
      661 cgcctgaaac gcgaacgcga cagcctgatc aaaaaattta gcgaactgcg tagctatgac
      721 gaccgcctgc gtcactatgc tgatatgaga atcagtattg atctcgacga tggcgttaag
      781 gttaactacg gcaagtttgg cgatctgctg gcagatgtca aagccatcac cggcaatgcc
      841 ccagaggcga tctaaaccag acggcgcgtt cccctactgc cgggttctgc ccggtggcaa
      901 ataccaccgg gaaacgcgcc gctgctggca tttctccacc tcacttcatg ataaaatgcg
      961 ccaccgtgtc aaaatctcct tttcgcgttt tggcgctttc ttattcatcg taacaacatg
     1021 ggattgtgaa cttgcaaaat caggacttta ttgctggcct taaagctaaa tttgccgaac
     1081 atcgcatcgt tttctggcac gatcccgata aacgttttat tgaggaactg gaacagctca
     1141 agcttgaaaa cgtcacgcta atcaacatga cccacgcgtc acagctggcg gtgaaaaaac
     1201 gcatcgagat ggatgagcca gaacagcagt tcctgctgtg gttcccccat gatgcgccgc
     1261 ctcatgaaca agactggctg ctggatatcc gcctttacag cagcgaattc catgccgatt
     1321 ttgccgccat caccctgaac acgctgggca ttccccagct tggcctgcgc gagcatattc
     1381 agcgacgcaa ggccttcttc agcactaaac gtacgcaggc gctgaaaaac ctggtgacgg
     1441 aacaggaaga tgaaacctcg ctggataaga aaatgattgc ggtgatcgct ggcgcaaaga
     1501 ccgcgaaaac cgaagacatt ttgtttaacc tgattaccca gtacgttaac caacaaacag
     1561 aagacgacag cgaactggaa aacacgcagg cgatgttgaa acgccacggt ctggacccgg
     1621 tattgtggga aatgctcaac cacgaaatgg gctaccaggc agaggagcca tcgctggaaa
     1681 acctgctcct gaaactgttt tgtaccgatc tctctgccca ggccgacaga cagcagcgcg
     1741 cctggctgga aaaaaatgtc ctgctgacgc catccggcag agcatccgcc ctggcattta
     1801 tggtgacctg gcgtgccgat cgtcgctata aagaggctta tgactactgc gctcagcaaa
     1861 tgcaggcggc cctgcgcccg gaagatcatt accgactcag ttcgccgtat gatttgcacg
     1921 aatgcgaaac caccctcagc atcgaacaaa ccattattca tgcgctggta acgcaactgc
     1981 tggaagagag caccacgctc gatcgggaag cctttaaaaa actgctctct gaacgccaga
     2041 gcaaatactg gtgccagaca caaccagagt attacgccat ctatgacgcg ctgcgccagg
     2101 ctgagcgttt gctgaatctg cgcaatcgcc acatcgacgg tttccactat caggacagcg
     2161 ccaccttctg gaaagcctac tgcgaagaac tgttccgctt cgaccaggct tatcgcctgt
     2221 ttaatgaata tgccctgctg gttcacagca aaggggcgat gatcctcaag agcctggatg
     2281 attatatcga ggcactctac agcaactggt atctggcaga attaagccgt aactggaatg
     2341 aagtgctgga agcggaaaat cgtatgcagg cgtggcaaat tcctggcgtg ccgcgtcagc
     2401 agaacttctt caatgagatg gtgaagccgc agttccaaaa tccgcaaatc aaacgcgtgt
     2461 tcgtgataat ttccgatgcc ctgcgttatg aagtggcgga ggagctgggg aatcaaatca
     2521 ataccgagaa acgctttacc gcagaactgc gatcgcagct cggcgtgctc cctagctaca
     2581 cccaactggg aatggcggcg ttgctgcccc atgaacaact ttgctatcaa cccggtaacg
     2641 gcgacatcgt ttatgctgat gggctgtcga cctcgggtat tcctaaccgc gataccattc
     2701 tgaagaacta taagggaatg gcgataaaat cgaaggacct tctggagtta aaaaaccagg
     2761 aagggcgaga ccttattcgc gattacgaag tagtgtatat ctggcataat acgattgatg
     2821 ccactggcga cacggcatcc acggaagata aaaccttcga agcgtgccgc acggcggtga
     2881 ctgaactgaa agatttagtc accaaggtaa tcaaccgtct ccacggcaca cgcatttttg
     2941 ttacggcgga tcacggtttc ctgttccagc aacaggcgct ttcggttcag gataaaacca
     3001 ctctgcaaat taagccggaa aacaccatca agaaccacaa acgctttatt atcggccacc
     3061 agcttcccgc cgatgatttt tgctggaaag ggaaagtggc ggataccgca ggcgtgagcg
     3121 acaacagcga gttccttatt ccgaaaggga tccagcgctt ccatttctct ggcggcgcgc
     3181 acttcgttca tggcggcacc atgttgcagg aggtttgcgt tccggtattg cagataaaag
     3241 ccctgcaaaa aaccgccgca gaaaaacagc cgcagcgccg cccggtggat attgtcgctt
     3301 accatccgat gattaagctg gtgaacaata tcgataaagt gagcctgttg cagacgcatc
     3361 cggtgggcga actttatgaa ccgcgtatcc tgaacattta cattgtcgac aacgccaaca
     3421 atgtggtctc gggcaaagag cgcatcagct ttgacagcga taacagcacc atggaaaaac
     3481 gcgtacgcga agttacgctg aagctgattg gcgctagctt caaccgtcgc aatgagtact
     3541 ggttgatact ggaagacgca caaacggaaa cggggtatca gaagtacccg gtcattatcg
     3601 atctggcgtt ccaggatgat ttcttctaag tgaggcgata tgcaaaccca tcatgattta
     3661 cctgtttcag gcgtatccgc aggggaaatt gcctccgagg gttacgatct ggacgccctg
     3721 ctgaaccagc attttgctgg tcgcgtggtg cgtaaagatc tcaccaagca actcaaggaa
     3781 ggggcaaacg tcccggtgta tgtgcttgag tatctgctcg gcatgtactg cgcctctgac
     3841 gatgacgacg tggtcgagca agggttgcaa aacgttaagc gtattctggc tgataactat
     3901 gtgcgcccgg atgaagcgga gaaagtgaag tcgctgatcc gcgagcgtgg ttcgtacaaa
     3961 atcatcgata aagtgtcggt gaaactgaac cagaaaaaag acgtttacga agcccagctt
     4021 tctaacctcg gcatcaaaga cgcgctggtg ccctcgcaga tggttaaaga caacgagaag
     4081 ctactgacgg gcggtatctg gtgcatgatt accgtcaact atttctttga agaagggcag
     4141 aagacctcac ccttctcatt gatgacgctc aagcctatcc agatgccgaa tatggatatg
     4201 gaagaagtgt tcgatgcgcg taaacacttt aaccgtgacc agtggatcga tgtgctgctg
     4261 cgctcggtgg gtatggagcc cgccaatatt gagcaacgca ccaaatggca ccttatcacc
     4321 cgtatgatcc cgttcgtgga gaacaactat aacgtttgcg agctggggcc gcgtggcacc
     4381 ggtaaaagcc atgtgtataa agagtgttct cctaactccc tgttagtttc cggcgggcaa
     4441 acgaccgttg ccaacttgtt ctacaacatg gccagtcgcc agatcggcct ggttggcatg
     4501 tgggatgtgg tagcgttcga cgaagtcgcg gggatcactt tcaaagataa agacggcgtg
     4561 caaatcatga aagattacat ggcgtcagga tctttctctc gcggcagaga ttcgattgaa
     4621 ggtaaagcgt cgatggtttt cgtcggcaac atcaatcaaa gcgtagagac tctcgttaaa
     4681 accagccatt tgctggcacc atttccgact gcgatgattg atacagcatt tttcgaccgc
     4741 tttcatgcct atattcccgg ttgggaaatc cccaaaatgc gcccggaatt ctttaccaac
     4801 cgttacgggc tgattacgga ttatctcgct gaatatatgc gcgaaatgcg caaacgcagt
     4861 ttctctgatg cgattgataa attctttaag ctgggtaaca acctcaacca gcgtgacgtt
     4921 attgccgttc gacgtaccgt gtcggggttg ttaaaactca tgcatcccga tggcgcgtac
     4981 agcaaagaag atgtgcgagt ctgcctgacc tatgcgatgg aagttcgccg ccgcgtgaaa
     5041 gagcaactta aaaaactggg cggtctggag ttcttcgatg tgaactttag ctacatcgac
     5101 aacgaaacgc tggaagagtt ttttgtgagc gtaccggaac agggcggcag cgaacttatt
     5161 cctgccggaa tgccaaagcc gggtgttgtg catctggtca ctcaggcaga aagcggcatg
     5221 accgggctgt atcgttttga aacacagatg actgccggta atggtaagca tagtgtatcg
     5281 ggtctgggtt caaatacctc cgcgaaagaa gctatccgcg tcggtttcga ttacttcaaa
     5341 ggcaatttga atcgggtaag cgcggccgcg aaattctccg atcatgaata tcaccttcat
     5401 gtcgttgaac tgcataatac tggcccaagc accgcaacca gtcttgctgc gcttatcgct
     5461 ttatgttcga tattgctggc aaaaccggtg caggaacaga tggtggtgtt gggcagtatg
     5521 acgcttggtg gggtaattaa cccggtgcag gatcttgccg ccagtttgca gctcgccttc
     5581 gacagcggtg caaaacgggt tctgttgccg atgtcctcgg ctatggatat tccaacggtt
     5641 ccggcagagt tatttaccaa gtttcaggtg agtttttact cagacccggt tgatgctgtt
     5701 tataaggcgc tgggtgtgaa ttaacgtagt aactatttta atgaactgga gaggacaatg
     5761 gctcaagaac gttctgtaat taaattcatc agaccttgcc tctccattca tgaatataaa
     5821 aatacttaca tctgagtatt gttggctgtg tttgaataaa aagagagctg ttcaaataag
     5881 cctgtataac tgttctttac aaaaaatgga ggagctatca gctaattcaa cactggaaga
     5941 tgttcgtcga gttggattga acgccctgcg cagaccaggc atttcggatt aagtgctcgc
     6001 acttccacag tcctggaaca agtctggctg gtagctgtaa acatactttg tacataccgc
     6061 tctggaatag atccccgtgc cacaggcttc acagaacttt ttcccgggaa aatgctgccc
     6121 gcacatcaca caatgccact ccagcacgac cggtaatggc gataaaaaca tcgccatatc
     6181 ctcaatgtaa gggtgggact tctccggatt cagcaccaca cagtccgcct tctgttgctc
     6241 actcagggca tgtaaatcat gctcaaacca cgccccctga gcatctgtct gcagaatcaa
     6301 ccggccatgg caggaaaggc agaaacactg ccggatattt ctgctgagcg cagagtcggc
     6361 actgataatg tgctcacccg gcgtgatccc cagccccgtt tttataccgt tcattctgct
     6421 cccccctcct cactgaagcg cccggtctgg cagtgtgtgc agtactgctc cccataataa
     6481 tcgtggtgac attgcctgca gtgccagctg gctttacgta ctacgggtaa ggcatccggg
     6541 acgaattgct gcagacgttt aatcaaccgt atttctctgc gttccggcct gacataaggg
     6601 cactcatgac cgtgctccgt cagcccttcg tcagcgtgtt caaaccaggg ccgttcagtg
     6661 tcgtattgcg ggtggtatct gagtgcactg ccgcaaaggt ggcaggtgta gctatcgtaa
     6721 ggtgcagtct gtgcggtacg ggcaccggtc agacgtccgt tgccatcaag tgcgagaaaa
     6781 gattttgcgt acatagtata tgttcctctg cgccagacga cacgcaggca acagccgtcc
     6841 cggtacgggc agcgtggtca gggtgtgaat ggcggttcag ttaaaatctg gactggagtt
     6901 tgggtaaagc aggctggttg ctgttacagc actgtggttg tcacacaatg gcccactgac
     6961 gtaaaaaaca gtcagtaagc agagatggag attgggggat gtgctgtatc ggttgccgga
     7021 aggaatggta aagaatgtgt catggttaaa tctccgtatg gttcatacgg aatgaccacg
     7081 ccgtaaaaaa tgcgcagtgc cgctgaagtg gccgccggaa ccgacagcaa agattagcgc
     7141 cagcccgtac ttgtcgttca ggggtttaca ccagagcaca tatccccggc agggggctgt
     7201 ccagtcgtgg tcgaccccgg ttactttttt caggaagccc gctgcatttc aggatgatgc
     7261 cacattcttt ctgttcccgt ttggccaggt agctgatggc atagcgtaaa tcattcaccg
     7321 ctttgtgatc tgaatagtga atgacgtggt taatgtttac cggataattt ttattaaacc
     7381 ggcaccagtg gtagtaacca ttaccctcgg taacccgcct ccagatatct cccatcaatc
     7441 tggataccag atacgaactc ctgtggctct ggccgttcag atagcccaca aaatgaatgt
     7501 gaagcccttt acgttctgta tattccatca cccaggcata cccgaccagt ttctctccca
     7561 cctcgttaat cagttgcagc atctccgcgc acatggcgta cctgcaggca catctgtagc
     7621 tgagggtgtt ttttcggtaa gcaaaatcca ttctgaacgg taacagtttc gaatagaccc
     7681 cgaataatga atccagatgc tggttaaagt gctccaggag ctggggagcc atttcgtaag
     7741 cgggattggt gttataagtt tgcatgtttc ttcctcgtgt tctgtaacac aggaagaagc
     7801 gttgccgtaa tttttgaaga agatgttctc aggtagtagg gtgaacgtgt ttcctccggt
     7861 aacgacttcc acgggtatca gaaccaggca aagaacagca tacgtaacag ccataca
//
LOCUS       DAZFRJ010000188         7905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000188 DAZFRJ010000000
VERSION     DAZFRJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.190"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..633)
                     /locus_tag="ACHKUN_003911"
     CDS             complement(<1..633)
                     /locus_tag="ACHKUN_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO6662084.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTV"
     gene            724..924
                     /locus_tag="ACHKUN_003912"
     CDS             724..924
                     /locus_tag="ACHKUN_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO6662085.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            968..1519
                     /gene="ymfL"
                     /locus_tag="ACHKUN_003913"
     CDS             968..1519
                     /gene="ymfL"
                     /locus_tag="ACHKUN_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO6662086.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            1665..1844
                     /locus_tag="ACHKUN_003914"
     CDS             1665..1844
                     /locus_tag="ACHKUN_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6662087.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            1774..2667
                     /locus_tag="ACHKUN_003915"
     CDS             1774..2667
                     /locus_tag="ACHKUN_003915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6662088.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGRG
                     NPEFTKAHNRALAVFLCHEQHYAQIMVGRAGPTSVGPGSLVTGISTPVRLTTNKVVES
                     LGGELLKITKEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTL
                     ECSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFN
                     QMEKQLSKLAVPSDVAHNASVLYSYISSIHQVWLQQLYPMLEKAESPLAVSLYDRIND
                     AAFLARLIHSSLNSSEVRGRK"
     gene            2664..2888
                     /locus_tag="ACHKUN_003916"
     CDS             2664..2888
                     /locus_tag="ACHKUN_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662089.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            2885..3703
                     /locus_tag="ACHKUN_003917"
     CDS             2885..3703
                     /locus_tag="ACHKUN_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662090.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARRDDPYFKASYDNVDYSQIPAGFRG"
     gene            3700..4194
                     /locus_tag="ACHKUN_003918"
     CDS             3700..4194
                     /locus_tag="ACHKUN_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6662091.1"
                     /translation="MIMSLLNDVQKFIEAHPGCASGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRQTEVLQESEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            4194..4847
                     /locus_tag="ACHKUN_003919"
     CDS             4194..4847
                     /locus_tag="ACHKUN_003919"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO6662092.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASSMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            4844..5170
                     /locus_tag="ACHKUN_003920"
     CDS             4844..5170
                     /locus_tag="ACHKUN_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO6662093.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            5167..5556
                     /locus_tag="ACHKUN_003921"
     CDS             5167..5556
                     /locus_tag="ACHKUN_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6662094.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            5576..6385
                     /locus_tag="ACHKUN_003922"
     CDS             5576..6385
                     /locus_tag="ACHKUN_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO6662095.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            6393..7382
                     /locus_tag="ACHKUN_003923"
     CDS             6393..7382
                     /locus_tag="ACHKUN_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6662096.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKVLRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            7400..7741
                     /locus_tag="ACHKUN_003924"
     CDS             7400..7741
                     /locus_tag="ACHKUN_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6662097.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
BASE COUNT         1976 a         1829 c         2167 g         1933 t
ORIGIN      
        1 tactgtacca attatagagc aattaccgtt aatcttaatg taaggctcag gccagtttgg
       61 gtttaatgct ttgagataac gctgtgttcc atcttctatc aatcgcttga aggtggtttc
      121 gcctgtatcg tgcatcaatg caataacgtc gtcaccgtgg caggcaggga cttcgggatc
      181 aacaaaaatc atgtctcccg ggcggtactc atcaatcatt gaatcaccaa tcacccgcaa
      241 gatataagtc atttcgccac agggtacagg gcagggataa gtttctgctg tgctcaaatc
      301 aacctcagaa tagccaactt ctttccatgc tccggcctgt acccatgata tgacagggac
      361 taacgttatt tgtttgttag tgattgaaac atcaggtttt tttgtgatgt tcgtggtctg
      421 gtgttcttga tcaagccatc cgacaggcag gtcgaaacat ttttcgatgt gccgtgccat
      481 gctgtcaccg atatttttag tagcaccatc tcccataaac ctgctggtct gggttggctc
      541 gcgatcaatc atggtggcaa aggaagaatt cccgccaaca ccatctctca gttttctggc
      601 gttagaccgc cggatgtcat ggactgtttt cataaagaaa ttaaaacctt tgtaccgata
      661 aggtacaagt atcttgaagg ttcatattaa tcatgtaata tgtatattgg aggtacatat
      721 tgtatgaaag cgtattggga ctctttaacc aaagaacagc agggcgagtt ggccggaaaa
      781 gttggctcaa caccaggcta cttacggctg gttttcaatg gttataaaaa agccagtttt
      841 gtgctggcta aaaaacttga gcaatgcacg tcaggtgcaa ttacgaaatc tgacttaaga
      901 ccggatatct atccgaaaga ttaacagaac accttcaatt tttaaccaca gaacgatgag
      961 gctaaccgtg ggtaagtatc actggaaagt agaaaaacag cctgagtggt acgtgaaagc
     1021 tgtcagaaaa actatcgcag cgttgccggg tggttacgct gaagcagctg actggctgga
     1081 tgtaacagag aacgcattat ttaaccgcct tcgtgccgat ggcgatcaga ttttcccgct
     1141 gggatgggca atggttttac agcgcgcggc tggcactcac tacattgcgg atgctgtcgc
     1201 acagtctgct ggtggggtgt tcgtatcgct tcctgaaatt gaggaagtag agaacgccga
     1261 tataaaccag cgcctgctgg aagtcatcga acagatcggg aattactcaa agcagattcg
     1321 ttcggcaatc gaagatgggg tcgtggagcc acacgagcag acagcaatta atgatgagtt
     1381 gtatctgtca atttcgaagc tccaggagca tgcagcactg gtctacaaaa tcttttgcgc
     1441 tccagaaaag agtgacgccc gcgagtgtgc agctccgggc gtcgtggcgt tttgtgtctg
     1501 tggagaaact aacgcatgaa cagtttaacg gcaaataacc gtttgtcgca acagctggtg
     1561 gtcagtgtcg ctgaacacct gttgttacgg catgaatgca gattaccaaa tcacctggct
     1621 gtaagtaacc acagagaact ttacctgact gtggggggcg agttgtgcag gaacttaacc
     1681 gctggtttcg tgacggaaga gggctttatg tccatgttat tcgttgggag ccagaaacac
     1741 agcgcgttat ctatcttcgc aaagactacc cgcatgagtg ctttagtcct ttgtggaaat
     1801 tcaggcgtga ttttgttgag tgtgaaggac caccagcaca ttgattctgc cattccggga
     1861 cgttacactg ttcaggcacc ttataaagcg ggtgccgggc gtggaaaccc ggaattcacc
     1921 aaagcgcaca accgcgctct tgcggttttt ttgtgtcatg agcagcatta cgcccaaatt
     1981 atggtggggc gtgcagggcc aacttcggtt gggccgggtt ctttggtgac cggtatttcc
     2041 acccctgtac gtctcaccac caataaggtc gtggaaagcc ttggtggtga gttattaaaa
     2101 atcaccaaag aggctgccat catggctacg atcccaaccc tcactcaacc cgaaattgcc
     2161 atcgttgatg gtcaggctgt tacttcatcc ctggctgttg ccaacttctt ctccaaacgt
     2221 catgacgatg ttctgaaaaa gatccgcacg cttgaatgct ccgcatcatt cactgcccgc
     2281 aatttttcgg tgagtgatta caccgattgc acaggccgca aactaccttg ctatcaaata
     2341 acccgcgacg gctttgcgtt tcttgctatg ggtttcacgg gtaaacgtgc tgcccagttc
     2401 aaagaggcat acatcaatgc ctttaaccag atggagaaac agctttcaaa gctcgctgta
     2461 ccgagtgacg ttgcacataa cgccagcgtt ctctattcct acatttcatc aattcatcag
     2521 gtctggttgc agcagcttta tcccatgctg gaaaaagctg aatcaccgct ggctgtaagt
     2581 ctgtatgacc gaattaacga tgcggcattt cttgcccgtc ttattcattc gtcgctgaac
     2641 tcttcagagg taagggggcg caaatgatcc gaaatatttt caaacggttt accaatcaaa
     2701 ctttccgttg tcctcgcccg ggtcagtggt acaccacgcc tgcagggcat gttctacgtg
     2761 ttagcctggt tgaccgtgaa tgtcagaagg tgatttgtga accgctgggt cgtaattacc
     2821 gcgtcagtat gccgctcata gcctttcgct ccggaaaaaa catgaagcat ctcggaggtg
     2881 cagcatgagt atggagctga tggttaaagc gatgaaaatt cgagtgggta atccattgcg
     2941 aaaactggtt ctgatcaagc tggctgataa tgccagcgat cagggtgagt gctggcccag
     3001 ctaccagcat attgctgacc agtgcgagat tagcaaacgt tctgtgatga atcatattgc
     3061 ggccctttgt gagtccgggc tggtaaaaaa agtcacccgg aaaggtgaaa aaggtaactc
     3121 aagtaatatc tatctccttc atctggatgg tgcaggagat tcactagggg gtagtgcaaa
     3181 taattcacta tctggtgcag caaattcacc aggtagtgca ggagttgcac cagggggtag
     3241 tgcaggagat tcacccagaa ccagtcactc ttttgaacca gtcaaagaac cagtcaatga
     3301 accaatagct gttggtgcat ctgctgatga gtctgtgcga gttcgttcaa accgaccgga
     3361 atactctccg gagtttgagc aggcatggct ggcatacccc aaacgtgctg gtggcaattc
     3421 aaaatcagca gccttcaaag cctggaaagc ccgtttgaac gagggggtaa accccgaaac
     3481 catgctggaa ggtgtgaaac gctacgcggg ctgggtatct gcgatgggca atagcggcac
     3541 acaatttgtg aaacaggctg tcacgttctt tggtccggat cgtcatttcg aagaatcctg
     3601 ggaagttcct gcggtatctg cagccagacg cgatgacccg tacttcaaag ccagttacga
     3661 caacgtggac tacagccaga tcccggcagg attcaggggg tgatcatgag tctgttaaat
     3721 gacgttcaga aattcattga agcccatccg gggtgtgctt ccggagacat tgcggatgct
     3781 tttgcaggtt actcacgaca gcgcgttctg cagtcagcaa gcaagttacg tcagagtggg
     3841 cgtgtggctc accgttgtga aggggatact cgcagacatt tcccgcgcca gactgaggtt
     3901 ttgcaggaat cggaaccgca accagttcgt gaaaccagac ctgtgcgcaa tttctatgtc
     3961 ggcactaacg acccccgggt gattttgtgc ctgacccgcc aggctgaaga actggagtcc
     4021 aggggcttat accgtcgtgc tgcaacggtg tggatggcgg cattccgtga aagccactcc
     4081 cagccagaac gaaacaattt tctggcgcgt cgtgagcggt gcttacggaa aagcagcaag
     4141 cgcgctgcat cgggtgaaga gtggtatctg tcagggaatt acgtgggggc ttaatgagta
     4201 ataaatattg ccaggcgctg gtggaactgc ggaacaaacc agcccatgaa ctgaaggaag
     4261 tgggcgatca gtggcgcacg ccggacaaca ttttctgggg aattaacacc ctgtttggcc
     4321 cgtttgttct ggatctgttt actgacggtg ataacgccaa atgtgccgcg tattacacgg
     4381 cggaagataa cgcgctggcg catgactggt cagaacgtct tgcggagctt aaaggtgctg
     4441 cctttggtaa tcccccatac agccgcgcca gtcagcatga ggggcaatac atcaccggca
     4501 tgcgttacat catgaaacat gccagttcca tgcgtgataa gggcgggcgc tatgttttcc
     4561 tgatcaaagc tgccaccagc gaagtgtggt ggccggaaga tgcggaccat attgctttta
     4621 ttcgcgggcg tattggtttt gaactgcctg cctggtttat cccgaaggac gagaagcagg
     4681 tgccgacagg agcttttttc gctggtgcta ttgctgtttt tgacaagacc tggaagggac
     4741 cggcaatcag ctacatcggg cgcgatgaac ttgaggcatg tggtgaggcg tttctggcgc
     4801 aggttcgcca gcaggcggaa aaactggtca gggagatggc ggcatgacga cgttaactca
     4861 atgccagcag caggtgctgg atatgctgat ttcttatcag aaagaacgtg gcttcccgcc
     4921 aaccaatcag gaggtggcaa ccatgctggg ataccgttcg gtgaatgcag cggtggagca
     4981 tcttcgcgca ctggagaaaa aaggcgtcat cacgataaag cgtggtgtgg cccgggggat
     5041 cacgcttcat accgcggtga aggacgacga cagcgaggcg gtcgggatta tccgctcact
     5101 gcttgccggt gaggaaaacg caaggctgcg tgcaactcac tggttacatg agagaggcct
     5161 gaaagtatga agctgatcct gcctttcccg cccagcgtga acacgtactg gcgacacccc
     5221 aacaaagggg catttgctgg taagagcctg ataagcgagg cggggcgaaa atttcagagc
     5281 gcggcgtgcg cagcaatagt tgagcagtta cgtcgtctgc cgaaaccaac gtcggcacct
     5341 gcttcagtgg agatcgtgtt gtttcctccg gataaccgga tccgcgatct ggacaactat
     5401 aacaaggcgc tgtttgacgc cctgacccac gcgggtgtgt gggaagacga cagccaggtg
     5461 aaaagaatgc tggtggagtg gggaccggtt atcccgaaag ggaaggtcga gatcactatc
     5521 agtaagtatg agaaaccggc gggtgcagcc gcctgattaa gaggagaaac gaagtatgaa
     5581 taatctgatg gtcattgatg gtattgaagt tcgtcgtgat gcttatgggc gttacagcct
     5641 gaacgatctg cacagggcag ccgggggaga acaaaaaaaa cgcccgaaat actggctctc
     5701 caataagcaa acctgtgaat tgattgaaca acttttcacc gagggtggaa ttccgcctct
     5761 ggaaaagaat caaccagtta gcgttattaa tggcggaaat aaccagggga cgtatgtctg
     5821 caaagaactg gtgtatgcct atgcaatgtg gatcagcccg tcattccatc tgaaggtgat
     5881 ccgtactttc gatatggtaa ccagcgcatc ggaaaaatta tccgggcagg ctgctgacaa
     5941 gatgcaggct ggcgtgattc tgctggactt tatgcgccgg gagttaaacc tgtctaactc
     6001 ttcagtgctt ggtgcctgtc agaaactcca ggaggctgtt ggcttaccga atctggcacc
     6061 gcgctatgcc attgatgctc ctgctgacgc gcctgatggc tcaagccgtc ccacgctgtc
     6121 actgagtgca ctgctgaagc agtatggtat ccgcctgaca gctaatcagg catatcacca
     6181 gatggtgaag ctggggatcg tcgagcagcg cgaacgatac agccgtaccg cgattaacaa
     6241 catcaaaaaa ttctggtcgc tgacagcgaa aggctgcatg ttcggcaaga acatcaccag
     6301 tcccgcaaat ccgcgcgaga cgcagccgca tttcttcgaa tcccgattcc ctgagctgtt
     6361 aaagctgctc gataccgttc attgaggtga ccgtgagagc actactgacc cctgaaattg
     6421 ccccgcgtat ggggatcgta ttgttcaggc caggttcaga gctgatgccc ctgtttatgc
     6481 aggggcgtgt cctgctggag cctgagccgg aacgttattc atctttcgcc agtggtgccg
     6541 ttccggcggc atcacaaccg ctggcggatg atcctgccgt tcgggccgtg ttccgcaatg
     6601 aggcagtgat ccgtcgtgct ggtggcgtgg aatgtcttga aagctggtta cttcgtgaaa
     6661 aaggctgcca gtggcctcat tccgactggc acagcgagaa catgaccaca atgcgacacg
     6721 cgccgggcgc aatccgtctg tgctggcact gcgataacca gctgcgcgat cagttcacgg
     6781 aacggctgga atcaatggca acggataact gtgcccgctg ggtgttgtct gtcgtgcgtc
     6841 gggatctcgg ttttgatgac agtcacgttg tgacaatgcc ggaactgtgc tggtggctga
     6901 ttcgtaatga tctggcggat gccttaccgg aaagtgcagc ccgtaaggta ctgagattac
     6961 cgaagcctgt tgtgccgtct gtcacccggg aaagtgacct tgtgccttcg gttcctgcca
     7021 ccagcatcat ccaggataaa gcgaaaaagg tgctggcgct gaaagtggat ccggagtcgc
     7081 cggagtcttt tatgttacgc ccaaaacgtc gccgctgggt taatgaaaag tacacgcgat
     7141 gggttaagac gcagccgtgc gcatgttgtg gaaaacctgc tgatgatccc caccacctga
     7201 taggtcacgg tcagggtgga atggctacaa aagcgcatga cctctttgtg ttgcctttgt
     7261 gcagaaagca tcacgacgag ctgcatgcgg ataccgtggc atttgaagag aagtatggct
     7321 cccagctgga gctgatattt cgttttatcg atcgtgcact ggcaattggc gtgctggcct
     7381 gattttgtgg agaaagttga tgcgtgatat gtatgaagta ttggaccgct ggggtgcatg
     7441 ggctgcagca gataacagtg gtgtggactg gcagcctgtt gctgcagggt ttaaaggttt
     7501 actacctcat ggaaagaaaa cacgccaaca atgcgatgat gatgaaggaa tcatgattga
     7561 cagctgtgtt gcgcgattgc ggaaatataa gcccgaagag tatgagttgg ttattgctca
     7621 ttttgttatc ggcatctcac taagaactat tgcaaagaag cagaagtgtt ctgatgggac
     7681 aataagaaaa gagttgcaaa cagctcttgg ttttctggat ggtgtgcttt caatgttgta
     7741 atattagggg gaattacccc cctttttttc tctgttgctt taataaaatt ttaatatttt
     7801 gtctgattat gatgaggcaa tgtaataaaa gaaataccgt tagtattgca agccatacgc
     7861 aaaataagca tgcatataaa ttagttgaaa gtccaatagt gaatt
//
LOCUS       DAZFRJ010000189         7831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000189 DAZFRJ010000000
VERSION     DAZFRJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.191"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..543
                     /locus_tag="ACHKUN_003925"
     CDS             19..543
                     /locus_tag="ACHKUN_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO6662098.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            627..1313
                     /gene="lpfB"
                     /locus_tag="ACHKUN_003926"
     CDS             627..1313
                     /gene="lpfB"
                     /locus_tag="ACHKUN_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO6662099.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1337..3868
                     /gene="lpfC"
                     /locus_tag="ACHKUN_003927"
     CDS             1337..3868
                     /gene="lpfC"
                     /locus_tag="ACHKUN_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO6662100.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3879..4934
                     /gene="lpfD"
                     /locus_tag="ACHKUN_003928"
     CDS             3879..4934
                     /gene="lpfD"
                     /locus_tag="ACHKUN_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO6662101.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4940..5464
                     /gene="lpfE"
                     /locus_tag="ACHKUN_003929"
     CDS             4940..5464
                     /gene="lpfE"
                     /locus_tag="ACHKUN_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO6662102.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5717..7408
                     /gene="eptB"
                     /locus_tag="ACHKUN_003930"
     CDS             5717..7408
                     /gene="eptB"
                     /locus_tag="ACHKUN_003930"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO6662103.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7500..7576
                     /locus_tag="ACHKUN_003931"
     tRNA            7500..7576
                     /locus_tag="ACHKUN_003931"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7534..7536,aa:Pro,seq:cgg)
     gene            7611..7744
                     /locus_tag="ACHKUN_003932"
     ncRNA           7611..7744
                     /ncRNA_class="other"
                     /locus_tag="ACHKUN_003932"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2037 a         1803 c         1988 g         2003 t
ORIGIN      
        1 atataaatgg agttataaat gaaaaaggtt ctgtttgcct tatctgcgct tgcgctgacc
       61 tctacttccg tattcgcagc tgatgcgggc gacggtactg ttaaattcac cggtgagatt
      121 gttgacgccg cttgtgttgt gtctccagac actcaaaaac aagaagttgt tctgggtcag
      181 gtgaacaagt ctgtatttac tgctatgggt gataaatcca cagcaacgcc gttcaaaatc
      241 aaactggaaa actgcgatat ctccactttc aaaaatgtgg aaatcagctt taacggcgtt
      301 ggcgatgcag acaacagcaa actggtttct gtaagcactg aaccaggtgc cgcaactggc
      361 gtaggtatcg gtatttatga taacaccaat accctggttg acctgaatac tggtaaatct
      421 gctactgtcc tgaaagaagg tcagaccgtt ctgtatttca ccgctaacta tgtctctacc
      481 aaagatgcag taaccacggg ttacggtaac gctgaagtcg acttcaacct gacttacaac
      541 taatcgatcc tgacggaacg atcatctctc aatggcaaca gagactgtgt tgccattatt
      601 cacaccaatg actcagggac cgaatcatga aacgtattag ctttgctgtg ctggcacttt
      661 taatgaccgc acaacagtgt tttgctggtg gcgtagcctt aagcacgacg cgtgttattt
      721 atgatggcgg caagaaagaa gcgtcgctga ccgttgaaaa ccataataaa aatgaagagt
      781 ttttgatcca gtcatggatt gatgatgtga atggcaataa aaaaacaccg tttattatta
      841 caccgccatt attcaaactt gatcctgata aaaataacgt cttacgtatc gtcaatattg
      901 aagatacctt acctaaagat cgtgaatccg tttactggat taatgtaaag gccattcctg
      961 cgaaaagtgc tgaaaatgaa aataaaaacg tcttacagat agccgttcgc acccgtttga
     1021 agttgttcta tcgtccaaaa ggtttgccgg gaagcaacct cgaggccatg aaacagttac
     1081 gtttccagcg tcaaaataac caaattgtgg ttaataacga ctccgcttat aacctgacct
     1141 ttaatcagtt ttccattaat ggacaaaaga ttgaacgggc gggaatggtt ctcgcgaaag
     1201 gaaagttaaa cattaacctt ccagccggta caggcaacgc gcacgaagtg aaatacagca
     1261 ttattaatga tttcggtgtt gctggcgaaa tgctgacgaa gccgattaac tgatcctttt
     1321 acaaggcgct tttactatgc attggatgca tttacctctc tatcactaca gagcgcattt
     1381 ttccttctct ctgctggcat taacgattgc cagctcgtta cctgcgtatg gcggaaaatt
     1441 taaccctaaa tttctcgaaa acgttcaggg tatcgatcaa cacatcgact tgtcggtata
     1501 cgactcacct gtcggccagc aaatccccgg taaataccgg gtttcggtgt ttgtaaacga
     1561 agaaaaaatg gcttcccgga cgctggattt tagtaccgcc agcgaagcga aacgcaaagc
     1621 cagcggtgag tcgcttatgc cttgccttag ccgggtgcaa ctggaagaaa tgggcgtgcg
     1681 cgttgacagc tttccggcgc tgaagatgtc accaccagaa gcctgcgtag cgtttgatga
     1741 aatcattccc caggccacca gccgctttga ttttaatact cagacgctgc atctttcctt
     1801 tccacaggcg gcaatgatga tgaccgctcg cggcacggtt gatccttctc gctgggacga
     1861 aggaatacct gcgctgttgc tggattacag ctttagtggc agcaatggac gcaatgaagg
     1921 tacaggttca tcttcagatt cgaccagtga cagttattac ctgaacctgc gcagcggttt
     1981 gaacgttggt ccgtggcgtt tacgtaataa cagtatctgg aaccgtaccg atggcaaaaa
     2041 ccaatgggat aacgttggta catcgttaaa ccgggcaatt attccactta aatcacagat
     2101 aactctgggt gataccgcca cgcctggcga gatttttgat agcgtacaaa tgcgtggggc
     2161 gttactggca tccgacgatg aaatgttgcc ggacagccag cgtggctttg ctccggtagt
     2221 gcgcggcatc gccaaaagca acgctgaagt gagtatcgaa cagaacggct atgtcattta
     2281 tcgcactttt gttcagccgg gtgcatttga gattaacgat ctctacgcaa cctccggcag
     2341 cggcgacctg acggtgatca ttaaagaagc cgacggaagt gaacaacgtt ttatccagcc
     2401 gttttcggcg gtggcgattt tccagcgtga agggtatctg aaatacagtc tggctgccgg
     2461 tgaatatcgt gctggtaatt acgacagcga taaaccacat ttcgggcagt ttaccgccat
     2521 gtacggcttg ccgtggggta tgaccgccta cggtggtgca ttgttgtcgg cagattacaa
     2581 cgctttagct ctgggcctgg ggaaaaactt cggtaccatt ggcgcagtat cggtggatgt
     2641 cactcaggcg aaaagtcagt tacgcaacaa tgaaaaagag gaaggccagt cttatcgctt
     2701 cctctattcg aaaagttttg agggtggcac tgacctgcgt ctgttgggct acaaatactc
     2761 aaccagcggc tactacacct tccaggaagc aactgatgtt cgcagtgacg ccgacagtga
     2821 ttaccgccgt tatcacaagc gcagccagat acagggcaat attacccagc aactgggaga
     2881 ctacggctcg gtttatttca atatgacgca gcaggattac tggaacgttg acggtaagga
     2941 aaactcctta agcgccggtt atcacggtca cattggtcgg gtgaattaca gcgtggccta
     3001 tacctggacg agaagtccgg aatgggaaga agacgatcgc ttgtggtcat tcagtgtttc
     3061 catccctctg gggggcgcgt ggagcagtta tcgcatgact accgaccaga atggcaagac
     3121 gtcgcaacag gcctctgtta gcggcacgtt actggaagat cgtaacctga gctataacgt
     3181 tcagcagggt tacaccagta atggcgtagg ctacagtggc agcgtcaaca tggggtatat
     3241 gggtggctct ggtaacattg acgtaggcta taactattct aaagataacc aacaggttaa
     3301 ctatggcgtg cgcggcggtg tcatcgttca cagtgaaggt ataacgcttt cgcagccttt
     3361 aggcgaaagt ctggcgattg tttctgcacc gggcgcgcgt ggtggacatg tggttaacag
     3421 cagcggcgta gaagttgact ggatgggcaa cgccgtggtg ccttatctca caccatatcg
     3481 tgaaaccatt gtggaattgc gtagcgacac gttggggcaa aacgttgaac tacaggaagc
     3541 gttccagaaa gtcgtgccta ctcgtggtgc agttgtgcgt tcccgctttg atacccgcgt
     3601 cggttaccgt gtgctgatga gcttaaaaca ggcgaatggc aacgcagtac cgtttggtgc
     3661 aaccgcagcg ttaattgatg agagcaaacc tgccagcagc attgtgggtg aagaaggaca
     3721 gctttatatc agcgggatgc cagaagaggg cgaattgcag gtgagctggg ggaacgagca
     3781 ggcgcaacgt tgccgtgtgc cattccgctt gccagagaac aaggacaatg cggctatcgt
     3841 gatggttaat gcggtatgcg agaaataagg atatttagat gaaaattaaa aatttgttgg
     3901 caggtatggt gctgttaggg acgagcactt ttgctggaaa tatatgggct gcggattggg
     3961 gaccatgtca gacatcaaat ggggtggccc atgagtatag ttttgatttc gtacagacta
     4021 tccaggtacc ttcagaaaat aaggctggga aaatattaac tcagcccttt gctttaggta
     4081 caaaatattc cgcttattgt gaatgcccgg atcccattcc agataatgga gttgtgactt
     4141 attttaaagg tgtaacgctt ctgcctgagc ctggaactgt agatgggtac tataaattca
     4201 ataattacat tgatattctc accaaaatat atgtctacac acaattagat atcccggtgc
     4261 cttttaccga tcgaagcaat ggcacggcac agaaagaatg tacgccctat actgctaaca
     4321 attgggggac aggtggtaaa ggaagtatca gtatttatat ttcacatcct tttgttgggc
     4381 aaatgattat tcctaaaaca cgagttgcgt ccctctttgg gacaaaaaag aaaggtgttt
     4441 acaatgatag tcagcccatg gccaatgtca gtatttccgg gagtattaca gttactcaag
     4501 gctgtgaact tgccgctggc accgtactgg atattccatt cggagaatat caggcccatg
     4561 attttaaagg gcgtgccgga caaccaccac aaaatgttca gaaggtacaa aaagaattaa
     4621 gctttgactg caataatatt tcagatggcg tcaaaattta tctcagtatt gatgcaacac
     4681 caaatactgc ctatcccagc gcaattgatt taggcaacgc cgatgttggt gcagtcattg
     4741 aggacggtaa aggtaatatt cttaagccga atgacagtaa tagcttatta gaaatgaacc
     4801 caggctcgct gtatgaagat gtcaaacgca aagcgacaac cacaattacc gcttatcccg
     4861 ttagcactac cggaaaatta cccgctgccg gggattatag cggtattgct accatacatg
     4921 tcgaactgga gtaacagtca tgaagcgaac aatacttgcg ttatgcctgc tgcctctttc
     4981 cttttcgtca ttcgcggcaa ccacggattt agggccgaaa ggaacattga aattcaccct
     5041 taaaatcagc cagggtgctt gcgaactggt gaaagacagc gttgaggttg acatgggcac
     5101 cgcggttctc aaaaagccgg taagaatcgg aacggaaatt aatcccactc ctttttccat
     5161 cggcctgaaa aactgtagcg aagtggtcag agcctacgtg actatggacg gtacacccaa
     5221 tgcagacgac tcaaatcttt ttgcgctgga tgcgggtggc gctacaggcg ttgggctgaa
     5281 aatcaaaacc gctgctggag tacaacagtt cccggaaaat acaacgccga caccggttga
     5341 gtttgcaatt aacggtgatg gcgcacatca actgaactat atcgccagct acgtgcctat
     5401 tcgcgccgat gcaactattg gtcgcgcaga tgcaacggtc gactttactg tacagtacga
     5461 ataatttcac atcttaaggg ccagttaact ggcccttttt actgcttttt gtaacgctaa
     5521 agtctctttt caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca
     5581 ctttttcgct ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc
     5641 tccgcacttt tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg
     5701 tccagggttt gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt
     5761 gcttgcaatc tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag
     5821 ctatgcgcac gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac
     5881 cgtactggtg accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg
     5941 tattctggcg tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct
     6001 taatgtggtc attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc
     6061 aaaagaagtt gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct
     6121 tatccttatc tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg
     6181 gcagcgtatt cgcagcctgg cggtcgtcgt actggcgggt attatggttt gggcaccgat
     6241 tcgtttgctg gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag
     6301 ttatggcggt gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct
     6361 gtatgcctgg gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa
     6421 gaaattcact tatcaggcac cgcaaaacgt tgatgacact tatgtcgtgt ttatcatcgg
     6481 tgaaaccacg cgttgggacc atatgggcat tttcggctat gagcgtaata ccacgccgaa
     6541 actggcccag gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa
     6601 actctcactg cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac
     6661 attaaaagaa cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta
     6721 cgctatgcag agcgaaatgt ggttctacag caacacgatg gcggacaaca ttgcttatcg
     6781 tgagcagatt ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt
     6841 agacgaaatg cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca
     6901 taccaaaggt tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa
     6961 gccggaatgt attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga
     7021 caactcggtg acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa
     7081 gaaagcgatt gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct
     7141 gcacggcacg ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg
     7201 gatgtcagat aaatatctgg aaaatccggc caatgctcag gcatttgcgc agctgaaaaa
     7261 agaagccgac atgaaagtgc cacgccgtca tgtagagctg tacgacacca tcatgggttg
     7321 tcttggctat acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc
     7381 gcagacaaaa gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg
     7441 cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc
     7501 ggtgattggc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat
     7561 cctctatcac cgaccaaatt cgaaaagcct gctcagcgag caggcttttt tgcatttgaa
     7621 gcccatgagg atgagaacct ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac
     7681 gttgcgccag caacggcccg cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg
     7741 accaaattcg aaaagcctgc tcaacgagca ggcttttttg catttgtagc tcatgaggat
     7801 gagaacctcc gggggcgggg aggttcgact c
//
LOCUS       DAZFRJ010000190         7711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000190 DAZFRJ010000000
VERSION     DAZFRJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.192"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..5169
                     /locus_tag="ACHKUN_003933"
                     /pseudo
     CDS             1..5169
                     /locus_tag="ACHKUN_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5392..5658
                     /gene="hipB"
                     /locus_tag="ACHKUN_003934"
     CDS             5392..5658
                     /gene="hipB"
                     /locus_tag="ACHKUN_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO6662104.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5658..6980
                     /gene="hipA"
                     /locus_tag="ACHKUN_003935"
     CDS             5658..6980
                     /gene="hipA"
                     /locus_tag="ACHKUN_003935"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO6662105.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7292..7500
                     /locus_tag="ACHKUN_003936"
                     /pseudo
     CDS             7292..7500
                     /locus_tag="ACHKUN_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2114 a         1645 c         2116 g         1836 t
ORIGIN      
        1 tctatcgcgt gatatggaat tgcactctac aggtatttca ggcctgctcg gaattaactc
       61 gcagggcagg taaaacatcg acggttaatt tgcgtaaatc ctctggactg acaacgaaat
      121 tcagtagatt gacgctgggt gttttgctgg cactaagcgg ttcagcgtct ggtgcaagtc
      181 tggaagttga taatgatcag attaccaata ttgatactga tgttgcttat gatgcctacc
      241 tggttggctg gtatggcact ggagtgctta atattttggc tggcggtaat gcctccttaa
      301 ccactattac taccagcgtc attggcgcta atgaggactc agagggcacc gttaatgttt
      361 tgggtggcac ctggcgattg tatgatagcg gaaataatgc aaggccttta aatgtgggtc
      421 aatccggaac ggggacgctg aatattaaac agaagggtca cgtcgatgga ggctatttaa
      481 gattaggttc ttcgacagga ggcgtcggga cggtcaatgt tgagggagag gactctgttc
      541 tgacgaccga attattcgaa atagggagtt acggtacggg ctcattaaat attacggata
      601 agggttacgt cacgagttca atcgtcgcca ttttaggcta tcaggcgggc agtaatggcc
      661 aggttgtcgt tgaaaagggt ggcgagtggc taataaaaaa taacgattcc tcaattgaat
      721 ttcaaattgg taatcaagga actggggagg cgactattcg cgagggtgga ctgattacgg
      781 ctgaaaatac gattatcggt ggcaatgcca ccggtgtcgg aaccctgaat gtgcaggatc
      841 aagactctgt catcacggta cgcagactct ataatggata tttcggtaat ggcacactca
      901 atatttccaa taatggacta attaataaca aagaatattc attagtgggc gttcaggacg
      961 gttcccacgg tgtcgtcaac gtgaccgata aagggcattg gaatttcctc ggaacgggcg
     1021 aagctttccg ctatatctat atcggtgatg ctggcgacgg tgaacttaat gtctcgcgtg
     1081 aaggtaaagt agattcggga attatcactg cggggatgaa agaaacaggc acaggcaaca
     1141 ttactgttaa ggataagaac tccgttatca ctaatctcgg aactaatctt ggttatgacg
     1201 gccacggcga aatgaatatc agtaatgaag ggcttgttgt cagcaacgga ggaagctcac
     1261 tcggttatgg agaaaccggc gtcgggaatg tcagcatcac cacggggggg atgtgggagg
     1321 tcaataagaa tgtctatacc accattggtg ttgcgggcgt cggaaacctc aatattagcg
     1381 atggcggtaa gttcgtatcg caaaatatta cttttttggg cgataaagca agcggtatcg
     1441 gcacactgaa cctgatggat gcgacatcat cgttcgatac tgtgggtatc aatgtcggta
     1501 attttggtag cggtatcgta aatgtcagta atggtgccac ccttaattca acgggctatg
     1561 gatttatcgg aggaaatgcc tccggtaagg ggatagttaa tatttcaacg gacagtctct
     1621 ggaatttaaa gacatcatct actaacgcac aattgctaca ggtcggtgta ttaggcaagg
     1681 gtgaactgaa tattaccacc ggaggtatag ttaaagcgcg tgatacacag atagctctca
     1741 atgacaaaag taagggcgac gtgagggtgg atgggcagaa ctctcttctt gaaacattca
     1801 atatgtacgt agggacatct ggtacgggta cgttaaccct gacgaatagc ggcacgctga
     1861 atgtcgaagg tggagaagtt tacttaggtg tttttgaacc tgctgtagga acgctaaaca
     1921 ttggtgcagc tcacggtgag gcggcggcag atgccgggta tataaccaac gcgacgaaag
     1981 tagagtttgg ttctggcgaa ggtgtttttg tctttaatca tactaataac agtgatgccg
     2041 gctaccaggt cgatatgctg attacaggtg acgataaaga cggaaaagtg atccatgatg
     2101 caggccatac ggtgttcaat gcagggaata cttatagcgg taaaacgctg gtcaatgacg
     2161 gcctcctgac cattgcgtct catacggcag atggggtaac gggcatgggt tcgagtgaag
     2221 tcaccattgc aagccccggt acgctcgaca ttctcgcatc aacgaacagt gcaggagatt
     2281 acacgctgac caatgcgctc aaaggcgatg gcttgatgcg agtgcagctg tcatcctacg
     2341 acaagatgtt tggctttacc catgcaacag ggactgaatt cgccggtgtt gcccaactga
     2401 aagacagtac cttcactctg gaacgcgaca acaccgctgc gcttactcac gcgatgttgc
     2461 agtctgacag tgaaaatacc acatcggtaa aagtgggaga gcaatccatt ggtggactgg
     2521 ccatgaatgg cggtacgctc attttcgata cggatattcc tgctgcgacg cttgcagagg
     2581 gatatatcag cgtcgatacg ctggttgtcg gcgcgggtga ctacacctgg aaaggccgta
     2641 actatcaggt aaacgggacg ggcgacgtgc ctaaaccgtg gaatgatccc atggcgaata
     2701 accctctgac gacgctcaat ttgctggaac acgatgatag ccatgtcggc gttcaactgg
     2761 tgaaggcgca aacggttatt gggtcgggtg gctcattaac gttacgtgat ttacagggcg
     2821 acgaggtgga agcggacaaa acgttacaca ttgcgcaaaa cggaacggtg gtcgccgagg
     2881 gtgattatgg attccgcctc acgaccgcac caggtgatgg tttgtacgtt aactatgggc
     2941 tgaaagcgct gaacatccat ggtgggcaaa agctgacgtt agccgaacat ggcggagcct
     3001 atggcgcaac ggccgatatg tcggcaaaaa tcggtggtga aggggatctg gcaatcaata
     3061 cggtgcgaca ggtttcgctt tccaacggtc agaacgacta tcagggggca acctacgttc
     3121 agatggggac attacgtacc gatgcggatg gtgcacttgg caacacccgg gaactgaaca
     3181 tcagcaacgc ggccatcgtc gatcttaatg gatcgacgca gacggtagag acattcaccg
     3241 ggcagatggg ttcgactgtt ttgttcaaag agggggcgct gacggtaaat aaaggtggga
     3301 tcagtcaggg tgaactgaca ggtggcggaa acctgaatgt tacaggggga acgctggcta
     3361 tcgaggggct taatgcacgc tacaatgcgt taaccagcat tagcccaaat gcggaagtca
     3421 gcctcgataa tacgcagggg ttaggcagag gaaatattgc caataacggt ctgttaacgc
     3481 taaaaaacgt gactggcgaa ctgcgcaata gcataagcgg gaagggtatc gtgagcgcaa
     3541 ccgccaggac agatgtagag ctggatggcg ataatagccg ctttgtgggg caattcaaca
     3601 ttgatacagg cagcgcgctc agcgtcaacg agcagaaaaa cctgggtgat gcttccgtta
     3661 tcaataatgg cctgctcacc atctccactg agcgtagctg ggcgatgacg cacagtatca
     3721 gcggtagcgg tgatgtgaca aaactgggta ccgggatcct gactcttaac aacgattccg
     3781 cggcgtatca gggtacgacg gatatcgtgg ggggggggga aattgctttc ggttccgact
     3841 ctgccattaa tatggcaagt caacacatta atatccataa cagcggtgtg atgtcgggaa
     3901 atgtcaccac tgcaggtgat gtgaacgtta tgcctggggg gacactgcgt gtcgctaaaa
     3961 ccacaatcgg cggcaacctg gagaatggcg gcacggttca aatgaatagc gaagggggga
     4021 aaccggggaa tgtactgacc gttaacggca actataccgg aaacaatggc ctgatgacgt
     4081 tcaacgcgac gctgggcggc gataattcgc ccaccgataa aatgaacgtg aaaggcgata
     4141 cccaagggaa cactcgcgtt cgggttgata acattggcgg cgtcggtgca caaacggtca
     4201 acggtattga actcattgag gttggcggta attctgcagg taacttcgcg ctgaccaccg
     4261 gaactgtcga agctggggct tacgtctaca cgctggctaa agggaagggg aatgacgaga
     4321 aaaactggta tctgaccagt aaatgggacg gcgtaacgcc agcggataca cccgatccca
     4381 tcaataatcc ccctgttgtg gatccggaag gcccatcagt ttatcgcccg gaggccggaa
     4441 gctatatcag caacattgcc gcagccaact cgctgtttag ccatcgttta cacgaccgtc
     4501 tgggtgagcc gcagtatata gattcactgc attctcaggg gtcggcaagc agtatgtgga
     4561 tgcgtcatgt cggagggcac gaacgttcaa gggccggtga cggtcagcta attactcagg
     4621 ctaaccgcta tgtattgcag ctaggcggcg atttggcgca gtggagtagc aacgcgcagg
     4681 atcgctggca tgacggcctc tgttgagggt gggtatacct ttgaagcggg aacatttaga
     4741 ggcagcgaag ggacgctgaa tacctggtac gtccagccac aggcgcaaat cacctggatg
     4801 ggtgtgaaag attctgacca taatcggaaa gacggaacac gcattgaaac ggaaggcgac
     4861 ggaaacgtgc aaacgcgact tggggtgaaa acctacctga atagccatca caagcgtgac
     4921 gatggtaaac agcgtgagtt ccagccttac attgaagcga actggatcaa caatagcaaa
     4981 gtctacgccg tgaagatgaa tggtcaaacc gtaagccgtg atggtgcgcg taatctcggt
     5041 gaagtacgta ccggggttga ggcgaaagta aataacaacc ttagcctgtg ggggaatgtc
     5101 ggtgtgcaac taggtgataa aggctatagc gatactcagg gcatgctggg agtgaaatat
     5161 agctggtaaa ccgtataagc cgcatgtcga gatggcatgc ggcttaatat tgccgacttc
     5221 aaacggcgca tcagcgcctt ttttaaatcc tcctttttat ccgcgatcgc ggatatcgca
     5281 gcgtttatcc cgtagagcgg ataagacgtg tttccaaatt gacttatcct cactaaagga
     5341 taaaactgat aatatcctct tatgcggata aatttgctgt ggacgtatga catgatgagc
     5401 tttcagaaga tctatagccc aacgcaattg gcgaatgcaa tgaaactggt tcgccagcaa
     5461 aatggctgga cgcagagcga gctggcgaaa aaaattggca ttaagcaggc gacaatttcc
     5521 aatttcgaaa acaaccctga caataccacg ctcacgacat tttttaagat tttacagtcg
     5581 cttgaactct caatgacact atgcgacacg aaaaatgcct cgccagaatc aacagaacag
     5641 caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag cgggtaggcg
     5701 agttaacgaa gttagccaac ggcgcgcaca cctttaagta tgcaccggag tggttagcca
     5761 gccgttatgc cagaccgttg tcactttcgc tgccattgca gagggggaat atcacctcag
     5821 atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta cgtgaccgga
     5881 tcgttaaacg ttatcatgct aaatccagac aaccgtttga tttattgtca gaaatagggc
     5941 gagacagcgt tggtgccgta acgttattac ccgaaaatga aaccataacg cgcccgataa
     6001 tggcatggga aaagcttact gaagccaggc ttgaagaagt attaacggct tataaagcag
     6061 atatccctct gggcatgatt agagaagaaa atgactttcg catctcggtt gctggcgcgc
     6121 aggagaagac agcgctgctt agaataggca atgactggtg cattccgaaa ggaataacgc
     6181 cgacgacgca catcattaaa ttaccgattg gcgaaatcag acagcccaat gcgacgctcg
     6241 atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa gaacttgggt
     6301 tgaatgttcc ggacgcagaa atcattaaag cgggaaatgt gcgcgcgtta gcggtcgaac
     6361 gttttgacag gcgttggaat actgagcgaa cggttttact tcgcttgcca caggaggata
     6421 tgtgtcagac attcggttta ccttcatctg tgaaatatga atcagatgga ggcccaggca
     6481 tcgcgcggat catggcgttt ttgatggggt ccagcgaggc gcttcgcgat cgttatgatt
     6541 ttatgaaatt ccaggtcttc cagtggttga ttggcgcaac ggatggccat gcaaaaaact
     6601 tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac gacatcattt
     6661 cagcatttcc ggtccttggc ggtacgggaa tacacatcag cgatctcaaa ctggcaatgg
     6721 ggcttaacgc atccaaaggc aaaaaaacgg caatcgataa aatttatccg cgacattttt
     6781 tggcgacagc aaaggtgctg agattcccgg aagtgcagat gcatgaaatc ctaagtgact
     6841 ttgccagaat gattccggca gcactggata acgtgaagac ttcattaccg acagattttc
     6901 cggagaacgt ggtgacggca gttgaaacca atgtgttgag gttgcatgga cggttaagcc
     6961 gagaatacgg tagtaagtga gatatggggt ttggtcgttg ttaatgagca cgacaatcat
     7021 gaccgccaac gactggattc ttccttggtt tatactggtg cgcataatgt atattatgtt
     7081 aaattatgtc tgagtgatgc gaagtgcttt tctggtagtc gttattcgtt cagggttcaa
     7141 gaacttaagc ctctcctctt tcataattgg cctgtgcatg gagaaatagc ctggttattc
     7201 cctgccccat gctaagagcg cagtgtctgt cgcatagaca ctgcatacga aaacaccaga
     7261 gggtgctgtt acttatttaa ggacggatca agcgggaatg agtccactcc ggtatcagaa
     7321 atggctacgt ctcaatgaag tcaggcgacc gatgctgaat gaacattacg atgtcaccac
     7381 agcagcctat gctgtcggtt acgaaagcta tcccatttca gtcgggaata ttcgcggatg
     7441 tttggagagt cacccaagag agatattacc aggttaagaa agtctgtcgg tcagctttaa
     7501 taaaccctgc acttatctgt ttagagcaat tcggtgttag tttcagcaag caaacattaa
     7561 ccatagctaa tgatttatag ccatattaac cattggtagc ccttgacctt tcctgacact
     7621 tcccttgttc gcaataatat tctgtttagt cacggtttat aaataacatg cataatgaaa
     7681 tcgtcatgtt atttagcgac ataaaatgtt t
//
LOCUS       DAZFRJ010000191         7621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000191 DAZFRJ010000000
VERSION     DAZFRJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.193"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..272
                     /locus_tag="ACHKUN_003937"
     CDS             <1..272
                     /locus_tag="ACHKUN_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO6662106.1"
                     /translation="MKPTAVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQKY
                     GHHGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
     gene            534..2156
                     /gene="yhiJ"
                     /locus_tag="ACHKUN_003938"
     CDS             534..2156
                     /gene="yhiJ"
                     /locus_tag="ACHKUN_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO6662107.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2346..2459
                     /gene="yhiY"
                     /locus_tag="ACHKUN_003939"
     CDS             2346..2459
                     /gene="yhiY"
                     /locus_tag="ACHKUN_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO6662108.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2522..3589
                     /gene="yhiI"
                     /locus_tag="ACHKUN_003940"
     CDS             2522..3589
                     /gene="yhiI"
                     /locus_tag="ACHKUN_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6662109.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3586..6321
                     /gene="rbbA"
                     /locus_tag="ACHKUN_003941"
     CDS             3586..6321
                     /gene="rbbA"
                     /locus_tag="ACHKUN_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO6662110.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6321..7445
                     /gene="yhhJ"
                     /locus_tag="ACHKUN_003942"
     CDS             6321..7445
                     /gene="yhhJ"
                     /locus_tag="ACHKUN_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6662111.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1895 a         1798 c         2033 g         1895 t
ORIGIN      
        1 ttatgaaacc cacagctgta tatgctgatt atattaataa cactactgat ttcagaccaa
       61 aacctgaacg tatcttcata tgtggagaga cactcaaatg tgaagatccc agcatataca
      121 ttagacaaaa atatggccat catggggtcg gggtagatca gaatcaacaa tttgataatg
      181 gtgttatggg gctaaattcc acgaagaaag tacttgataa aaataacaaa ataatttatt
      241 cctcagggct tagttgtttt caatctcatt aaaactccat gaatacggtg gtatgccttt
      301 ttaatcctgc cagtatttca taggttttct taatcgattg tagaggtatg tactgatgac
      361 gcctctacaa tctttaggga ctttctctgc tatgagataa gaaatttcat attatgcttt
      421 aaggctgaat gatttgtgca aaatataatt atttttccat cgaacatgag gtgtatgtca
      481 taagattaaa tgttaactat gcattcctcg cggtgaatta tatgaggtaa aagatgaaaa
      541 ttggaactgt ggcaggcact aacgacagta caacgacaat cgcaacaaat gatatggtgc
      601 aagagcatgt tactaacttc actaaagaac tgtttgggta tattgcaaat ggtataggtg
      661 atgatatatc aagtattgcc aggacaatgc tcggtgaagt tgtggaaaaa attgatgatt
      721 ggcaaataga acgtttccaa caatctattc gagatgacaa aatttcattt accatacaaa
      781 cgaatcattc agaaaaatat agtatgctat cgggcatgcg cgctcatatt ctacgtaggg
      841 ataataatta tcagtttatt gtgacaatta atagtataaa ttacggctgc tatcttgata
      901 ataccgatat taattggtgc agcattgttt atttacttaa taatatgaca gtaaatgatg
      961 atgctaatga tgttgcagtt actgagtcgt acaaacccat ctggaactgg aaaatatcgc
     1021 aatacaatgt atttgatatt aaattcgaaa caatgataaa accccagttc gctgacagaa
     1081 cctatttctc caattgttca cctgttgatc ccacaagtac cagaccaacc tattttggcg
     1141 ataccgatgg ctcagtcggt gcagttttgt acgcgctttt tgcaacggga catttgggaa
     1201 tcatggctga aggagaaaat ttccttagcc agttgcttaa tatagaagat gaagttctca
     1261 acgtattgct aagggagaac tttaatgagc aacttgacac taatgttaat ataataatca
     1321 gtatcctgaa cagaagagac attgttttag aaagtcttca gccttatttg gttattaata
     1381 aagacgcagt cacgccatgt acgtttctgg gcgaccagac tggcgatcga tttagtaata
     1441 tctgtggcga taaatttatc atcgatttat taaaacgcat aatgagtatc aatgaaaacg
     1501 ttcatgtatt agctggaaat catgaaacga attgcaatgg taactatatg cagaacttca
     1561 cgcgcatgaa accacttgat gaggatacct acgctgggat aaaagattat cctgtatgtt
     1621 tctatggccc caaatacaaa ataatggcaa atcatcatgg gataactttt gatgatcagc
     1681 gaaaacgtta cattatagga ccgataacag tctctagtga tgaaatgacc aatgctctcg
     1741 atccggtcga attagcggca attatcaata aaaagcacca tgctgtaatt aatggcaaaa
     1801 agtttaaaaa tagccgggct atctcttgtc gatcctttaa tcgttatttt tctgtttcga
     1861 cagattacag acctaaactt gaagcactat tagcatgctc acaaatgtta ggtataaatc
     1921 aagttgttgc acataacggt aatggtggaa gagaatgtat aggtgaaacg gggacagttt
     1981 taggtcttaa cgcccgtgac agtaaacatg ctggaagaat gttcagcatg cataattgcc
     2041 aaattaaccc tggtgcaggg cctgagataa ccactccctg gaagtcttac cagcatgaaa
     2101 aaaacagaaa tggacttatg cctttgatta gaaggcgcac aatgttacag ctctgaacca
     2161 tagacaacat gaatattaat gttactatct ggttgagact gttgataaaa cgtaaaaagg
     2221 aatgctctgt tcctgcaact gaacaggcgt gaaacgcgat ccaacaggat ggataccgtt
     2281 ttgtcagcca gttttataga tcacacttat gagtatgcgt gtttcattta cggccaggaa
     2341 tgctgatgat gacaacatta ctacccgttt tcactaagcc ttccccgttg gcgctcaatg
     2401 ctctgcgcgc tggtcgtatt tgtcgtttcc ttcttatccc ggatggaaga atccgctgaa
     2461 cgtcgttcgg cggcagttgt aacacgcgtc gtttgcttac agggattatg gcgggccgat
     2521 tatggataag agtaagcgcc atctggcgtg gtgggttgtc ggggcactgg cggtggctgc
     2581 cgtcgtggcg tggtggctgt tgcgcccggc gggtgtgccg gaaggctttg ctgtcagcaa
     2641 tgggcgcatt gaagcgacgg aagtggatat cgccagcaaa attgccgggc gtatcgacac
     2701 cattctggtg aaagaaggcc agtttgttcg cgaaggtgaa gtgctggcga agatggatac
     2761 tcgcgtgttg caggaacagc gactggaagc catcgcgcaa atcaaagagg cacaaagcgc
     2821 cgttgccgcc gcgcaggctt tgctggagca acgacaaagc gaaagtcgcg ccgcgcagtc
     2881 gctggttaat caacgccagg cagaactgga ctccgtagca aaacgtcata tgcgttcccg
     2941 ttcactggcc caacgagggg ctatttctgc gcaacagctg gatgacgacc gcgccgccgc
     3001 tgagagcgcc cgagctgcgc tggaatcggc aaaagctcag gtttctgctt ctaaagccgc
     3061 tatagaggcg gcacgtacca atatcattca ggcgcaaacc cgcgtcgaag cggcacaagc
     3121 cactgaacgg cgcattgccg cagatatcga tgacagcgaa ctgaaagccc cgcgtgacgg
     3181 acgcgtgcag tatcgggttg ccgagccagg ggaagtgctg gcggcaggcg gtcgggtgct
     3241 gaatatggtc gatctcagcg acgtctatat gaccttcttc ctgccaaccg aacaggcggg
     3301 cacgctgaaa ctgggtggtg aagcccggct gatcctcgat gccgcgccag atctgcgtat
     3361 tccggcgacc atcagttttg tcgccagtgt cgcccagttc acgccaaaaa ccgtcgaaac
     3421 cagcgatgaa cggctgaaac tgatgttccg cgtcaaagcg cgtatcccac cggaattact
     3481 ccagcagcat ctggaatatg tcaaaaccgg tttaccgggc gtagcgtggg tgcgggtgaa
     3541 tgaagaactt ccgtggcctg acgacctcgt ggtgaggttg ccgcaatgac gcatctggaa
     3601 ctggttcccg tcccgcctgt cgcgcaactg gcgggcgtga gccagcatta tggaaaaacc
     3661 gttgcgctga acaatattac tctcgatatt ccggcccgct gtatggtcgg gctgattggt
     3721 ccggatggcg tcgggaagtc gagcttattg tcgttgattt ccggtgcccg cgtcattgag
     3781 cagggcaatg tgatggtgct gggcggcgat atgcgcgacc cgaagcatcg ccgcgacgtc
     3841 tgcccgcgca tcgcctggat gccgcagggg ctgggcaaaa atctctacca caccttgtcg
     3901 gtgtatgaaa acgtcgattt tttcgctcgc ctgttcggtc acgacaaagc ggagcgggaa
     3961 gtgcgaatta atgagctgct gaccagcacc gggttagcac cgtttcgcga tcgtccggca
     4021 gggaaactct ccggcgggat gaagcaaaaa ctggggctgt gctgtgcgtt aatccacgac
     4081 ccggaactgt tgattcttga tgagccaaca acgggggttg actcgctctc ccgcgcccag
     4141 ttctgggatc tgatcgacag tattcgccag cggcagagca atatgagcgt gctggttgcc
     4201 accgcctata tggaagaggc cgaacgcttc gactggttgg tggcgatgaa tgccggagaa
     4261 gtgctggcaa ccggcagcgc cgaagagttg cggcagcaaa cgcaaagcgc cacgctggaa
     4321 gaagcattta taaatctgtt accgcaagca caacgccagg cgcatcaggc ggtagtgatc
     4381 ccaccgtatc aacctgaaaa cgcagagatt gccatcgaag cgcgcgatct gaccatgcgt
     4441 tttggttcct tcgttgccgt tgatcacgtc aatttccgca ttccacgcgg ggagattttt
     4501 ggattccttg gttcgaacgg ctgcggtaaa tccaccacca tgaaaatgct taccggactg
     4561 ctgcccgcca gcgaaggtga ggcgtggctg ttcgggcaac cggttgatcc aaaagatatc
     4621 gatacccgcc gtcgggtagg ctatatgtcg caggcgtttt cgctctataa cgaactcacc
     4681 gtgcggcaaa accttgagtt acatgcccgt ttgtttcaca tcccggaagc ggaaattccc
     4741 gcgcgagtgg ctgaaatgag cgagcgtttt aagctcaacg acgttgaaga tgttctgccg
     4801 gagtcattgc cgctcggcat tcgccagcgg ctttcgctgg cggtggcggt gattcatcgc
     4861 ccggagatgt taatcctcga tgaacctact tctggcgtcg atccggtggc gagggatatg
     4921 ttctggcagt tgatggtcga tctctcgcgc caggacaaag tgactatctt catctccacc
     4981 cactttatga acgaagcgga acgttgcgac cgcatctcac tgatgcacgc cggaaaagtg
     5041 cttgccagcg gtacaccgca ggaactggtt gagaaacgcg gagccgccag tctggaagag
     5101 gcatttatcg cctatttgca ggaagcggca gggcagagca acgaagccga agcgccgccc
     5161 gtggtacacg acatcaccca cgcgccgcgt cagggattta gcctgcgccg tctgtttagc
     5221 tacagccgcc gcgaagcact ggaactgcgc cgcgatccgg tccgttcgac gctggcgctg
     5281 atgggaacgg tgatcctgat gctgataatg ggttacggca tcagtatgga tgtggaaaac
     5341 ctgcgctttg cggtgctcga ccgcgaccag accgtcagta gccaggcgtg gacgctcaat
     5401 ctctccggtt cccgttactt tatcgaacag ccgccgctca ccagttatga cgagcttgat
     5461 cgtcggatgc gtgcgggcga tatcacggtg gcaattgaga tccctcccaa tttcgggcgc
     5521 gatatcgcgc gtggtacgcc tgtggaactc ggcgtctgga ttgacggagc gatgcccagt
     5581 cgtgccgaaa cggtaaaagg ttacgtgcag gcaatgcacc agagctggtt acaggatgtg
     5641 gcgagccgac aatccacacc cgccagccaa agcgggctga tgaacattga gacgcgttat
     5701 cgctataacc cggacgtaaa aagcctgcca gcgattgttc cggcggtgat cccgcttctg
     5761 ctgatgatga tcccgtcaat gctaagcgcc cttagcgtgg tgcgggaaaa agagcttggg
     5821 tcgattatca acctttacgt gacccccacc acgcgtagtg aatttttgct tggtaaacag
     5881 ttgccataca tcgcgctggg gatgctgaac tttttcctgc tctgcggcct gtcggtgttt
     5941 gtgtttggcg taccgcataa aggcagtttc ctgacgctca ccctggcggc gctgctgtat
     6001 atcatcattg ccaccggaat ggggctgctg atctccacct ttatgaaaag ccagattgcc
     6061 gccattttcg gaacggcgat tatcacgttg atcccggcga cacagttttc cgggatgatc
     6121 gatccggtag cttcgctgga agggcctgga cgttggatcg gcgaggttta cccgaccagt
     6181 cattttctga ctatcgcccg cgggacgttc tcgaaagcgc tggatctgac tgatttgtgg
     6241 caacttttta tcccgttgct gatagccatc ccgctggtga tgggcttaag tatcctgctg
     6301 ctgaaaaaac aggagggatg atgcgccatt tacgcaatat ttttaatctg ggtatcaaag
     6361 agttgcgcag tctgctcggt gataaagcga tgctgacgct gattgtcttc tcgtttacgg
     6421 tgtcggtgta ttcgtcagcg accgttacgc caggatcgtt gaacctcgcg ccgatcgcca
     6481 ttgccgatat ggatcaatcg cagttatcga accggatcgt taacagcttc tatcgtccgt
     6541 ggtttttgcc accggagatg atcaccgccg atgagatgga tgccggactg gacgccggac
     6601 gctatacctt cgcgataaat attccgccta attttcagcg tgatgtcctc gccggacgcc
     6661 agccagatat tcaggtgaac gtcgatgcca cgcgcatgag ccaggcattt accggcaatg
     6721 ggtatatcca gaatattatc aacggtgaag tgaacagctt tgtcgcgcgc taccgtgata
     6781 acagcgaacc gttggtatcg ctggaaaccc ggatgcgctt taacccgaac cttgatcccg
     6841 cgtggtttgg cggggtgatg gcaatcatca acaacattac catgctggcg attgtattga
     6901 ccggatcggc gctgatccgc gagcgtgaac acggcacggt ggaacacttg ttggtgatgc
     6961 cgataacgcc gtttgagatc atgatggcaa aggtctggtc gatggggctg gtggtgctgg
     7021 tggtgtcggg attatcactg gtgctgatgg tgaaaggcgt gctgggtgta ccgattgaag
     7081 gctcgatccc gctgtttatg ctgggcgtgg cgctcagtct gtttgccacc acgtcaatcg
     7141 gcatttttat ggggacgata gcgcgttcaa tgccgcaact ggggctgctg gtgattctgg
     7201 tgctgctgcc gctgcaaatg ctttccggtg gatccacgcc gcgcgaaagt atgccgcaga
     7261 tggtgcagga cattatgctg accatgccga cgacacactt tgttagcctc gcgcaggcca
     7321 tcctctaccg gggtgccgga ttcgaaatcg tctggccgca gttcctgacg ctgatggcaa
     7381 ttggcggcgc gtttttcacc attgcactgc tgcgattcag gaagacgatt gggacaatgg
     7441 cgtaaataca attgcccgat gcgtaagctt atagggacta cgcatcggtg caatttattg
     7501 aattttagtt gtttttaggc cggataaggc gtttatgaac tgacccccaa aacctggaca
     7561 gtttcagtta accggctttt tgatactcag tcctgtattc agggcaagat tacgaaagcc
     7621 c
//
LOCUS       DAZFRJ010000192         7582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000192 DAZFRJ010000000
VERSION     DAZFRJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.194"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            399..1406
                     /locus_tag="ACHKUN_003943"
                     /pseudo
     CDS             399..1406
                     /locus_tag="ACHKUN_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2026..3535
                     /locus_tag="ACHKUN_003944"
                     /pseudo
     CDS             2026..3535
                     /locus_tag="ACHKUN_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3586..3700)
                     /locus_tag="ACHKUN_003945"
                     /pseudo
     CDS             complement(3586..3700)
                     /locus_tag="ACHKUN_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3787..4263
                     /locus_tag="ACHKUN_003946"
     CDS             3787..4263
                     /locus_tag="ACHKUN_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6662112.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            4244..5396
                     /locus_tag="ACHKUN_003947"
                     /pseudo
     CDS             4244..5396
                     /locus_tag="ACHKUN_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            5459..6908
                     /locus_tag="ACHKUN_003948"
                     /pseudo
     CDS             5459..6908
                     /locus_tag="ACHKUN_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6975..7467)
                     /locus_tag="ACHKUN_003949"
                     /pseudo
     CDS             complement(6975..7467)
                     /locus_tag="ACHKUN_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2003 a         1814 c         2029 g         1736 t
ORIGIN      
        1 aggcagccta gcggctgcgg cttgtcatgg cctgaaatta ccgttataaa aacaaacaat
       61 gtcattgtct ttcaggtagt tatatggcac gttcagctaa accccgtaaa cgaaaactcg
      121 cacctcaaag aagcaaactt ccccgctatg ttgtaaaact tcacgaagat gatttttttg
      181 acgaagaaga agtagagatt ctgcgcttcg ataattttga cgatactgtt gagtgttgcg
      241 cagacctgaa tattccgttc tttgtggatg ccggaaacaa aaagctggtc ttctggtttg
      301 tacgtgttga tgacgaaggg catccggaaa tcgcccgctg tacagagcgg gagtttacga
      361 ccattcttgc cggtatcagc gccggcggca tgtactgccc ggactgttgc aatacaaaga
      421 aggtctgttt tcggtgtaaa aaacgctcct gcccacactg cggggtgaaa gcgggtgcac
      481 agtggataca gtatctgctg agtctggtcc ccgacggccc gtggcagcaa ttgtattcac
      541 gcttccctgc cagtactggt ccctggtgtt ccacaaccgg tggttactgg cagagatgag
      601 ccgcatagca gcagatgtga agccggggat attcacggta atccacacat ggggccggga
      661 ccagcagtgt catccacata tccacatatc catttatcca tttatcgaca actgccggcg
      721 gtgtgacatc acatcacacc tggaaaaacc ttcattttta cgcccgtaag gtgatgagta
      781 tgtagcgata ccggataaca cggttactgt cacggaaata cccggaacta ttgataccgg
      841 atgagctggc tgctgaagga agcagtaaac gggaatggaa tcgtttcctg gacagtcatt
      901 accgacgggg caggaatgtc aacgtatcaa aggtgatgga taacgccaca catgtggcgg
      961 tgtactttgg ctcttacctg aaaaagccgc cggtgccaat gagccgtctg gagcactatg
     1021 ccggtcagga tgaaatcgga ctgcgttaca acagtcaccg cacacgacgt gaagaatacc
     1081 tattgatgag tggtgatgcg tttatggaaa ggttctcctg gcatgtggcg gataaggggt
     1141 tccgtatggt gaggttctac gggttcctta gtccggcgaa acgccggttg ctggaagaat
     1201 tagtgtacgt aataacagag acggtgataa aaacggcgat gcaaatcagg tggagaggga
     1261 tgtatcagcg gttactgaag gttgacccgc tgaaatgcat cctgtgcgaa agtcagatgc
     1321 ggtttacggg gctgaagcgg ggttaccgtc tggcagagct ggttatgatg catgagccgc
     1381 tggcccgaat gcagtattgc ggctgagagc cgcagagggg aagttgcgtc cattttgagg
     1441 gaaatggagc aaaaaaacgt caccgatacc ctgtatcaat caatgtcagc aatataagcg
     1501 ctgatatata cccgatgagg ttccggatgt gttgaataaa tgttcgtttt ttgttttctt
     1561 aatctttccg ggggataaat tcctgtcgat gaacatatca aaatcaggaa attacacgaa
     1621 tctatgtaca ggctctctgg gaacacttta cgctttgaca cagagggcta aataatgagc
     1681 gattcagatg ccatttttgc atacagagga ttgacagcat tcccatgcta attgttaagg
     1741 tttaaattga agtatatcta tctgtcattt ttgcaaatga catgcggtag cataacgttc
     1801 cgaggtctct tatgcagaca tacgtggcga cattaaccac tcccagatac agccagcctt
     1861 catccgtacg taagtacgtg atgtctcgcg cccacttctg gctctggcta ctggcgtaaa
     1921 aatcctgctc ctgtcacgaa cggtgcaaaa gtggttcttc cgcaacctgg gtggagaaaa
     1981 tggatgaaac tttatggtga acaaggatct aagctgtttt tagttatggg taacgcaatg
     2041 cactctctta aaacacttct acagttacct tgcggatggc gatgtagtcg acaaattatt
     2101 aactctgacg gtatacccct ccatctccgc ggaaaacgca aaacagcaca atgtcctgaa
     2161 tgctttaagc gtagcgactc tgttcatagt tgtcgtcggc gccggataca gcatctaccc
     2221 tgctcagggc agacgctatg gcttgtattt tccgtccgcc acaggtacag ccgtaacccg
     2281 gcttgttcac gaaaaatttt tgccgagtcg tttgttccct tcggtggttc acatctgcag
     2341 tcttcacagg cgttacaaaa tttacagcgt caactgggat taatcgccgg aggtgaggcc
     2401 ggaaaacggg cagcaacggc agtgggtctc cgttgcagtg ctgatactct ccttcgcagg
     2461 gttatcaata ccccggagac gaaacagtca ggcgcgcctc atgtcggtat tgatgagtgg
     2521 gcgtggcatc ggggccaccg ttacggtacg ttaatcgtca atcttgatac tcaccgtccc
     2581 ctcgtcctgc ttcccggccg tgatcagcgt acgctggcga cctggttcag aaaatatccg
     2641 gaaatacaga ttgtctcgcg tgatcgcagt ggggtctgtg cgacagcagc acgtgaaggt
     2701 gtaccacagg ccagacaggt ggtcgatgcc acctgctaaa aaatattggc gatgcgcttg
     2761 aacgaatgat gtacagacat atacctctga tacgtcttgt tgccagcgag ttgtcaccaa
     2821 agaaaccacc tgagccagaa ctgtctgagc ctgcagcatc gctccgtcgt ccggaacgcc
     2881 taaaacaaca aacccgcaaa aaacggcatc agcgttggac agaggttatg gcccagcata
     2941 acaagggatg tagtttcagg gaaatatccc gtattacagg cctgtcgcgt gtgacagtca
     3001 gtcgctgggt gcgttcagga acattccctg aaatgtcaac ccgacctcca aagcgagggc
     3061 ttctggaccc atggagggag tggttaaaag agcaacgaga aagtggtaat tataacgcca
     3121 gccggatatg gcgggaaatg gtggcccggg taagccgctg gttgatgccc tggagaataa
     3181 ccagggatga agaaaattat gcttcccgat ttattagtct gatgtgtgaa aagaacctga
     3241 gctgaaaata gcgcagcaac tggcactcga gttctaccgt attctgaaaa cccaaaataa
     3301 atcacagcta agtagttggt tcactcgagt ccacgaaagc ggttcagcag aatttcggcg
     3361 agtggcggcg gggatggaag ctgatgctgc agctatatgt gaggcaatca gtagtcgctg
     3421 gagtaatgtt gttgtcgaag gtcatgtaaa tcgcctgaag atgctgaaac gccagatgta
     3481 tggtcgagca ggatttgaac tactcaggca gagggtaatg agtccactgg catgaaaatc
     3541 tccacccagg ttgcggaaga ggcacttttc atccgtcgtt gacagctcca gcagattttc
     3601 tgacacaggc aggctgtgtg cggtaagggc tgaacttccg ggaagccttt gccctcagtc
     3661 cgtgacggca caggcttgcc atcacggttt ttacgttaaa agtaagcgta aaccgaccgc
     3721 agtatgtagc cattagacaa gaattggtaa cttagacgcc catctgacac agacggacat
     3781 ccaagtatgg aattacagga ctggcgaaaa gaacctcgta aaaagtattc gaatgaattc
     3841 aaacttcgta tggttgaact ggcatcacaa cccggtgctt gtatggccca gattgcacga
     3901 gaaaatggcg tcaatgatga tgttattttc aaatggctca gactctggca aaacgaaggg
     3961 cgtatatcac ggcgtcttcc ggtgacaacc acttctgacg caggcgttga attattacct
     4021 gtagagataa cgcctgatga gccgaaagaa ccgatggctg ctcttactcc atccttatcc
     4081 actcagacta ctgtcagtgc cagctcctgc aaagtggagt tccgtcacgg taacatgacg
     4141 ctggaaaatc cttcaccaga gctgctcact gtgaagtggc acactgaatt tggccacctg
     4201 aacagaggtg atatgctcac ctcagaacaa cacaggtgct ctaatgaaaa aaagaaattt
     4261 tagcgcagag tttaaacgcg aatccgctca actggttgtt gaccagaaat acacggtggc
     4321 agatgccgcc aaagctatgg atgttggcct ttctacaatg acaagatggg tcaaacaact
     4381 gcgtgatgag cgtcagggca aaacaccaaa agcctctccg ataacaccag aacaaatcga
     4441 aatacgtaag ctgaggaaaa agctacaacg cattgaaatg gagaatgaaa tattaaaaaa
     4501 ggctaccgcg ctcttgatgt cagactccct gaacagttct cgataatcgg gaaactcaga
     4561 gcgcattatc ctgtggtcac actctgccat gtgttcgggg ttcatcgcag cagctacaga
     4621 tactggaaaa accgtcctga aaaaccagac ggcagacggg ctgtattacg cagtcaggta
     4681 cttgagctac atggcatcag ccacggttcg gccggagcaa gaagcatcgc cacaatggca
     4741 acccggagag gctaccagat gggacgctgg cttgctggca ggctcatgaa agagctgggg
     4801 ctggtcagct gtcagcagcc gactcaccgg tataaacgtg gtggtcatga acatgttgct
     4861 atccctaact accttgaaag gcagttcgcc gtgaccgagc caaatcaggt gtggtgcggt
     4921 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
     4981 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
     5041 aaagcgcggg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
     5101 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
     5161 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
     5221 aggagtctga agagaacgaa tggatgccgg tggtgggtta cgtaagcttc agcgaggcag
     5281 ctcacgccat aacggactat atcgttggat attacagcgc actaagaccg cacgaatata
     5341 acggtgggtt acccccaaac gaatcggaaa atcgatactg gaaaaactct aactaggtgg
     5401 ccagtttttg ttgaccactt cagaaagcca gttccgtcag aaagaccagc aactaagtct
     5461 ggttgaagag acggaggcct tcctgtgctc tgcactggca cgtgccgaag aaaagatcga
     5521 agaagatgaa cgggaaatag aacatctgca gactcagata gaaaaactgc gccggatgct
     5581 gttcggtacc cgttctgaaa aactgcgtcg tgaagttgaa caggctgagg ccctgctgaa
     5641 acaacgcgaa caggacagcg atcgttacag tgggcgggaa gacgctccac aggttccccg
     5701 ccagttgaga cagccgcgcc atcgtcgtcc gttaccggca caccttcccc atgcaataca
     5761 ccgtctggag tctgaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctgga
     5821 ggaagtcagt gcagaacaac tggaactggt gagcagcgcc ctgaaagtga tccgcactgt
     5881 tcgggtaaaa aagcctgtac aaaatgtgac tgcatcgttg aagcaccggc gccgtcccgc
     5941 ccgatagagc gtggtatcgc gggccccgga ttacttgccc gcgtgttaac gggaaaatac
     6001 tgcgaacagc tgccactgta tcgtcagagt gaaatctttg cccgacaggg tgtcgaactg
     6061 agccgtgcat tactctccaa ctgggttgac gcgtgctgcc agttaatgac tccgctgaat
     6121 gatgccctgt accgttatgt gatgaatacc cgcaagcttc acactgacga cacaccggta
     6181 aaggtactgg cactgggcct gaaaagacga aaacagggcg catctggacg tatgtccgga
     6241 atgatcgcaa tgcgggttcg tcatctcctc cggcggtctg gttcgcgtac tcaccgaacc
     6301 ggcaggggaa acacccggag caacacctcc gccccctgca ggcggatgcg ttcaacggtt
     6361 acgatcggct gttcagtgca gaacgtgaag atggtgcgct gacagaagtt gcgtgctgag
     6421 cccatgtccg gagaaaaatc cacgatgtat acatcagcag caaaagtgcg acggcagaag
     6481 aagccctgaa gcgcatcagt gaactgtacg ccatcgagga tgaaatacgg ggattaccag
     6541 agtcagagcg tcttgaagtc aggcagcagc gaagcaaagc gttactgacg tcgctgcatg
     6601 aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagaccaggc gaagcgttca
     6661 gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt ctggcggagg
     6721 cggacaataa cacagcggaa agagcgcttc gtgccgtctg tctcgggaaa aagaattaca
     6781 tgttcttcgg tagcgatcac ggcggcgagc gtggagcact gctgtacggg ctgatcggca
     6841 cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg agcgtactac
     6901 cggaatagcc ctccaaccgt gttgacgaac tcctgccatg gaacgtagta ctcaccaata
     6961 aataagcgtc aatacggtgc tccgtagcgc tgttttgacc ggataaaagc cgcgagtacc
     7021 acgctgtcgc agtgctgacg gaactgctga catgtactta tccttgtccg ccgctgacac
     7081 cacatatacc agtcactgcg gccttcctgg agcacatggc acattgcctt gatgttgaac
     7141 acagcctgat gtttttcaat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg
     7201 cggccttttg gaggatggtc agctcttcat cccgttccgc cagctggcgt ttgagacgtg
     7261 caacatcggt agacatctcc agctcacgtt cagaagaacg tctactgatt ttgttgttga
     7321 ctacccagtt gtagactgtg attcatacag gctgagctca cgggctgcag cagcaatacc
     7381 tatactgttg tgaattgctg gcgctgcttc cctctttttt tcattggtat attgaccact
     7441 gacgaatggg gtagtcacat cagatatgtt atagaaggag actcatctat ttttattcag
     7501 catattgagt gcatgagaat attaatcaaa catctgaccc gtagggctat ctgtttcccg
     7561 cactctgtaa atttcataaa aa
//
LOCUS       DAZFRJ010000193         7519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000193 DAZFRJ010000000
VERSION     DAZFRJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.195"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            212..4465
                     /gene="fdeC"
                     /locus_tag="ACHKUN_003950"
     CDS             212..4465
                     /gene="fdeC"
                     /locus_tag="ACHKUN_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO6662113.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWHDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(4586..5443)
                     /locus_tag="ACHKUN_003951"
     CDS             complement(4586..5443)
                     /locus_tag="ACHKUN_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662114.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5692..6561
                     /locus_tag="ACHKUN_003952"
     CDS             5692..6561
                     /locus_tag="ACHKUN_003952"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6662115.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6721..7314)
                     /gene="rclC"
                     /locus_tag="ACHKUN_003953"
     CDS             complement(6721..7314)
                     /gene="rclC"
                     /locus_tag="ACHKUN_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO6662116.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7326..7517)
                     /gene="rclB"
                     /locus_tag="ACHKUN_003954"
     CDS             complement(7326..7517)
                     /gene="rclB"
                     /locus_tag="ACHKUN_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO6662117.1"
                     /translation="MAFSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSW
                     KVTSMRIDNNSTATAVLYK"
BASE COUNT         2052 a         1679 c         1928 g         1860 t
ORIGIN      
        1 cccccataaa taaaaatata atcccgcgcc caaccacctg atgagtggct acaggcactg
       61 gatatattag gtggcggtgc actttcttac ataaaggtat ttccttttct gcggaaaagg
      121 aaatcgggaa atccccggtt tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt
      181 tcgttaattc gttcatcagg aaattatctc aatgtcacat tataaaacag gtcataaaca
      241 accacgattt cgttattcag ttctgacccg ctgcgtggcg tgggcaaata tctctgttca
      301 ggttcttttt ccactcgctg tcacctttac cccagtaatg gcggcacgtg cgcagcatgc
      361 ggttcagcca cggttgagca tgggaaatac tacggtaact gctgataata acgtggagaa
      421 aaatgtcgcg tcgtttgccg caaatgccgg gacattttta agcagtcagc cagatagcga
      481 tgcgacacgt aactttatta ccggaatggc cacagctaaa gctaaccagg aaatacagga
      541 gtggctcggg aaatatggta ctgcgcgcgt caaactgaat gtcgataaag atttctcgct
      601 gaaggattct tcgctggaaa tgctttatcc gatttatgat acgccaacaa atatgttgtt
      661 cactcaggga gcaatacatc gtaccgacga tcgtactcag tcaaatattg gttttggctg
      721 gcgtcatttt tcaggaaatg actggatggc gggggtgaac acctttatcg accatgattt
      781 atcccgtagt catacccgca ttggtgttgg tgcggaatac tggcacgatt atctgaaact
      841 gagcgccaat ggttatatcc gggcttctgg ctggaaaaaa tcgccggatg ttgaggatta
      901 tcaggaacgc ccggcgaatg gctgggatat tcgtgctgag ggctatttac ccgcctggcc
      961 gcagcttggc gcaagcctga tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg
     1021 taaagataag cgccagaaag acccgcatgc tatttctgcc gaggtgacct atacgccagt
     1081 gcctcttctg acactgagcg ccgggcataa gcagggcaag agtggtgaga atgacactcg
     1141 ctttggcctg gaagttaatt atcggattgg cgaacctctg gcgaaacaac tcgatacaga
     1201 cagcattcgc gagcgtcggg tactggcagg cagccgctat gacctggttg agcgtaataa
     1261 caacatcgtt cttgagtacc gcaaatctga agtgatccgt attgctctgc ctgaacgtat
     1321 tgaaggtaag ggtggtcaga cactttccct ggggcttgtg gtcagcaaag caactcacgg
     1381 actgaaaaat gtgcagtggg aagcgccgtc attactggct gagggtggca aaattaccgg
     1441 tcagggtagt cagtggcaag taacgctccc ggcttatcgt ccaggcaaag acaattatta
     1501 tgcgatttct gcggttgcct acgataacaa aggcaatgcc tcaaaacgcg tgcagacaga
     1561 ggtggtcatt accggagcag gtatgagcgc cgatcgcacg gcgttaacgc ttgacggtca
     1621 gagccgtatt caaatgcttg ctaacggtaa tgagcaaaga ccgctggtgc tgtctctgcg
     1681 cgacgccgag gggcagccag tcacgggcat gaaagatcag atcaagactg aactagcctt
     1741 caaaccggct ggaaatattg tgactcgttc cctaaaggcc actaaatcac aggcaaagcc
     1801 aacactgggt gagttcaccg aaactgaagc aggggtgtat cagtctgtct ttactaccgg
     1861 aacgcagtca ggtgaggcaa cgattactgt tagcgttgat ggcatgagca aaaccgtcac
     1921 tgcagaactg cgggccacga tgatggatgt ggcaaactcc accctgagcg ctaacgagcc
     1981 gtcaggtgat gtggttgctg atggtcagca agcctatacg ttgacgttga ctgcggtgga
     2041 ctccgagggt aatccggtga cgggagaagc cagccgcttg cgatttgttc cgcaagacac
     2101 taatggtgta accgttggtg ccatttcgga aataaaacca ggcgtttaca gcgccacggt
     2161 ttcttcaacc cgtgccggaa acgttgttgt gcgtgctttc agcgagcagt atcagctggg
     2221 cacattacaa caaacgctga agtttgttgc cgggccgctt gatgcagcac attcgtccat
     2281 cacactgaat cctgataaac cggtggttgg cggtacagtt acggcaatct ggacggcaaa
     2341 agatgctaat gacaaccctg taactggtct caatccggat gcaccgtcat tatcgggcgc
     2401 agctgctgta ggttctacgg catcaggctg gacggataat ggcgacggga cctggactgc
     2461 gcagatttct ctcggcacta cggcgggtga attagaggtt atgccgaagc taaatggaca
     2521 ggatgcggca gcgaatgcgg ctaaagtaac cgtggtggct gatgcgttat cttcaggcca
     2581 gtcgacagtg tctgtagccg cagatcgcgt aaaagcaggc gaaagcacaa ccgtgacgct
     2641 tattgcaaaa gatgcacatg gcaacgctat cagtggtctt tccctgtcgg caagcctgac
     2701 gggtgctgcg tctgaagggg cgactgtttc tggttggacc gaaaaaggtg atggttccta
     2761 tgtcgctacg ctgacaacag gtggaaagac gggtgagctt ctcgtcatgc cgctattcaa
     2821 cggccagcca gcagccaccg aagccgcgca gttgactgtc attgcggggg agatgtcatc
     2881 agcgaactct acgcttgttg ctgacaataa ggctccgacc gtcaaaacga cgacgaaact
     2941 caccttcacc gtgaaggatg cgtacgggaa ccttgtcacc gggctgaagc cagatgcacc
     3001 gcagtttagt ggtgccgcca gcacggggac agagcgacct tcaacaggag actggacaga
     3061 aacaagtaat ggggtctacg tggcgacctt aacgctggga tctgccgcgg gccagttgtc
     3121 tgtgatgccg cgagtgaacg gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc
     3181 tggtgacgca tctaaggctg agattcgtga tatgacggtg aaggttgata accagctggc
     3241 taatggacaa tcgactaacc aggtaaccct gaccgttgtg gacacctatg gtaacccgtt
     3301 gcagggacaa aatgtgacgc tgactctgcc gaaaggtgtg accagcaaga cggggaatac
     3361 ggtaacaacc gatgcggcag gtaaagccga cattgagctg atgtcaacgg ttgccgggga
     3421 acacagcatc acggcctcag tgaataatgc tcagaagacg gtcacggtga aattcaacgc
     3481 ggatgccagc accggtcagg caaacctgca ggtagacacc gctgctcaaa aagtggcaaa
     3541 cggcaaagat gcctttacgc tgacggcgaa cgttgaggat aaaaatggta accctgttcc
     3601 agggagcctg gtgaccttta atctgccccg gggtgtcaag ccgcttacag gcgataatgt
     3661 ctgggtgaaa gccaacgatg aggggaaagc cgagttgcag gtggtttcag tgactgccgg
     3721 aacgtatgag atcacggcat cggcagggaa tagccagcct tcgaatacgc agactataac
     3781 gtttgtagcc gataaggcta ccgcaaccgt ctccggtatt gaggtgatgg gcaactatgc
     3841 gctggcggac ggcaaagcca aacaaacgta taaagttacg gtgactgatg ccaataacaa
     3901 tttggtgaaa gatagcgaag tgacgctgac tgccagcccg gcttcgttaa acctggaacc
     3961 gaatggcact gcgacaacga atgagcaagg gcaggctatt ttcaccgcta ccactactgt
     4021 tgcggcgaca tacacactca aggcgcaagt gagtcagacc aacggtcagg tatcaacgaa
     4081 aactgccgaa tctaaattcg ttgcggatga taaaaacgcg gtactcaccg catcatctga
     4141 tatgcaatct ctggtggcgg atgggaaatc gactgcgaag ctggaggtga cactgatgtc
     4201 ggcaaacaac cccgttggcg ggaatatgtg ggtcgacatt cagacgccgg aaggggtgac
     4261 ggaaaaggat tatcagttcc tgccgtcgaa aaatgaccat ttcgtgagcg gaaaaatcac
     4321 gcgtaaattt agtaccagca agcctggtgt ctatacgttc acatttaacg ccctgaccta
     4381 tggcgggtac gaaatgaagc cagtgacggt gaccattacc gcggtggatg ccgatacggc
     4441 aaaggacgag gaggcgatga aataatttta agaatatgaa agtaacattc taataaacaa
     4501 taatggcgtt gacatcttca acgccattat ttattgaatt tcctgacagt agtctcgtta
     4561 taaattcatt tagatatagc gaaaactaaa ccggacgaac ggggataaaa atttcgcaaa
     4621 caatgttatt gctatcaatt tcgacgaaat tttcattgta ataaaaccgc tcaatatcac
     4681 tgccgttcac tctggctaac cccctggcgg gtaattccat cagatagagg tttcgcgccc
     4741 attcagggta gtgctcccac tcaccttcat agcggaaaga ggcatacagt cctcggttaa
     4801 ttgtccactc tttcggcatc tctgaatcgg tttgtttgca taccgtaccg gcaaaagttt
     4861 caaccgtgag caaatcgacc gattttgttg acggttcaaa cgtggagacg aaatagatat
     4921 ccgtcttaaa attattcata catctggcta aatgatgacg tcgcagtttg acttcttcat
     4981 ccggtgattt attcgacact tcaatatcat atttaaatga attgccaaaa ataggtgtct
     5041 caggaaagtt gactaatcta cactcggtct tttgctgttg acgtattaaa aaagaaggga
     5101 agatatttgc gagatcccaa taatcacggt ggcggtatac acggggagag cagccgaata
     5161 actttttaaa ttcgcggctg aatgactgct gtgaatcaaa atgcaaagag agtgcaatat
     5221 caagcataga tttcgcggtg agccggacaa gaatggcggc acgacaaagc cttcgtttgc
     5281 gaatgtattc tcccaaaggc acatgcatga aatttcggaa cagaagctgg atgttgcgtc
     5341 tgctgtagcc agatttctgt gcgatatctt cgattgaaat agggtgctca agattgcact
     5401 caatccactc caggagctgc tgtagaatct tctgcctgat catcacattc tccattgatt
     5461 cttcgtcata tcgcacattt tcgttatgcc ctgggcccta agtgtatcaa aaacagcaag
     5521 ggcgaaggca aaagcactgg tagcgtctgc ttcgttgtcc ccgaaaatag agaggtttca
     5581 gtcctgcatt attaatgaat tttttgcata agtgatatca aaatccacgt actaatttga
     5641 ggttacgttt taacgtagac tcattgttca tgcctaatgg agggactgac agtggaattt
     5701 tccgtattaa gtaacaatct gaaaatgccg atgatgggat ttggtgtttt tcaggttacc
     5761 gataaaaacg tgtgcaaaca gtcagtgttg aacgctatcc gcacaggtta tcgactcatc
     5821 gacactgcag cggtatacgg taatgaagat gccgtcggtg aagccgttcg tgaagctata
     5881 tctgaaggtt tatgtacccg agatgagtta tttataacgt ctaaattgtg ggtgcaggat
     5941 atgttgaatc aggatacggc agcagcaggt attgaagcat cattaaaaaa atcgggacta
     6001 gagtacttcg acctctattt attgcaccag gctatgcgcg attatttcag tgcgtggcgt
     6061 gcactcgaag atgcctatga agagggcaaa ttaaaagcaa ttggggtttc caatttctat
     6121 cctcatgttc tggcgaactt ttgtgaaacg gtaagagtta aaccgatggt caaccaggtc
     6181 gagttgcatc cctattttgc ccaaccagag gcgctggcaa ccatgaagta ttataacgtg
     6241 cagcctgaag catgggctcc gttaggtggt ggacgacata aaccctttga aaataatctg
     6301 cttcagagta ttgcagatgc ccatcaaaaa tcgatttctc aagtcattct gcgttggaat
     6361 attcaacggg gagtggtcgt tattccgaaa tcgacacatc aacagcgtat cgaagaaaat
     6421 tttgctatct gggatttctc actgacagag aaagaaatgg cacaaattag ttcgcttgat
     6481 ttgggttatg ttggggagtc ggtaaaacat tttaatcctg aatttgttcg tggttgtctt
     6541 gctgtaaaaa tacatgattg atattaatca tatattttac ctgagacgac aagaatcttt
     6601 taacagggga gtgatattga tcttcactct gtcatatctc cggtaatatg gcgtcaggct
     6661 tcatcatcag aaagggggcc tgacgcctga aaaagtgaac aacagacagt gttcggatta
     6721 tcaatattca gtttttaacg ttgaactgga ttcattactg cgttgtttaa gaatttcccg
     6781 cgccgaatct gccattatca tgacagcacc tgccagcatc agagtatctt tcaataccag
     6841 gcgaccagca ccggataaat aagggaaacc atgatgagcg tcacccaatg cgggtaccca
     6901 tgcctccggg gtggtgatta aaaatgagag tgttaccagc ggcgtggtaa atgccatcag
     6961 tcctcccaat aaacctaacc agcgattgac aggattagcc aaaaccagca gagcaataat
     7021 cacctccacg acgccaagac cgttggaaaa accataggta ttattggccg tttgccatgc
     7081 ccttgcttct ggtttgtatt cgccttcgtg agtcagatac tgtttatagt cttccgggtg
     7141 ttcataaaag aacgacatta gtggactgtt tgcgacgaat ggtgtaatgc tgtctgcctc
     7201 gtaagggaca aactttaata acccaatcca cataaaaaca attgcaatac tcagacgaat
     7261 caatgtcagg ccaattttat ctccccgact taacaggtgc aggtattttt ccattgtgaa
     7321 catccttatt tatacaatac tgctgttgcg gttgaattat tatcaattcg catcgatgtg
     7381 actttccagg atgaagcatt atattttctg gctatatcag caatggtatc ctccagaact
     7441 gtcggtgatg ctgatactga cgagacgctg atatgtttca gaattatttt atcatctgca
     7501 ttggtggaaa atgccataa
//
LOCUS       DAZFRJ010000194         7483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000194 DAZFRJ010000000
VERSION     DAZFRJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.196"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..328
                     /gene="chaB"
                     /locus_tag="ACHKUN_003955"
     CDS             98..328
                     /gene="chaB"
                     /locus_tag="ACHKUN_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO6662118.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            486..1181
                     /gene="chaC"
                     /locus_tag="ACHKUN_003956"
     CDS             486..1181
                     /gene="chaC"
                     /locus_tag="ACHKUN_003956"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO6662119.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1225..1578)
                     /gene="ychN"
                     /locus_tag="ACHKUN_003957"
     CDS             complement(1225..1578)
                     /gene="ychN"
                     /locus_tag="ACHKUN_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO6662120.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1763..3157
                     /gene="ychO"
                     /locus_tag="ACHKUN_003958"
                     /gene_synonym="iatA"
     CDS             1763..3157
                     /gene="ychO"
                     /locus_tag="ACHKUN_003958"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO6662121.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3158..3808)
                     /gene="narL"
                     /locus_tag="ACHKUN_003959"
     CDS             complement(3158..3808)
                     /gene="narL"
                     /locus_tag="ACHKUN_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO6662122.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3801..5597)
                     /gene="narX"
                     /locus_tag="ACHKUN_003960"
     CDS             complement(3801..5597)
                     /gene="narX"
                     /locus_tag="ACHKUN_003960"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO6662123.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5936..7327
                     /gene="narK"
                     /locus_tag="ACHKUN_003961"
     CDS             5936..7327
                     /gene="narK"
                     /locus_tag="ACHKUN_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO6662124.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         1738 a         1843 c         1969 g         1933 t
ORIGIN      
        1 tttcttaaga acagtctgac atgaaaattc ttatgttttg gcaagagtag atattgttga
       61 ccacacttaa tgttcaactt tgtaaaagga gtcaacgatg ccgtataaaa cgaaaagcga
      121 tctgccggaa agcgtaaagc acgttctacc gtctcatgcc caggatatct ataaagaagc
      181 gttcaacagc gcatgggatc aatataaaga taaagacgat cggcgtgatg acgccagtcg
      241 cgaagaaacc gcgcataaag tggcctgggc tgctgtgaag catgaatatg ccaaagggga
      301 tgatgataaa tggcataaaa aatcgtaaaa ccggtagctt agttaaagct attcgtgcgg
      361 tgttgccttg caagtggtcc gtggattgca tattgtcccg ttagtggttt caaaatgagc
      421 agtaaaaatg tccggaagac accaaaaagt tgtcgcaggg aagtatgcag tggcggaggt
      481 gtaaggtgat aacgcgtgat ttcttgatga atgccgattg taaaacggca tttggtgcca
      541 ttgaagaatc actcttatgg tcagcagagc aacgggcggc ttcgctggcg gcgacgctgg
      601 cttgtcgacc tgatgaggga ccggtgtgga tcttcggtta tggatcgttg atgtggaatc
      661 cggcactgga gtttaccgag tcgtgcaccg gtacactggt tggatggcat cgcgcgtttt
      721 gcctgcgcct gaccgccggg cgcgggactg cgcaccagcc gggacggatg cttgcactga
      781 aagagggcgg acgcactaca ggcgtcgcct atcgactgcc agaagagacg ctggagcagg
      841 aactaaccct gttgtggaag cgcgagatga ttaccggctg ttatctgcca acctggtgtc
      901 agcttgatct tgatgatgga tgcacagtaa acgccattgt gtttattatg gacccgcgac
      961 atccagaata tgaatctgac actcgcgctc aggtcatcgc gccgttgatt gcggcggcga
     1021 gcggtccgct gggaaccaac gcacaatacc tgttttcact ggaacaggag cttatcaaac
     1081 tgggaatgca ggacgatggg ctgaatgatt tgctggtatc ggtaaaaaaa ctgctggcgg
     1141 agaattatcc ggatggtgtg ttacggccgg gattcgcctg agtaaacttc cctcatagtg
     1201 gggcgtcaga cgccccctca aacattaaaa tgtgagcact ttatcggctg acagcgtcca
     1261 ttgcgccagt tccacaagag taccgatttc caccccatca atcagaggaa gtgtactaat
     1321 cccgcgcccg tcggtacagg ttttgcacaa tttcaccggt acattctgag cggtaaggat
     1381 ctccagcatt tgctgaatgt tgtagccttc ccctggtttt tgcccgcgca acccggctgt
     1441 gaccgcatca gacatcagga acagacgcag atccagattg ctctcctgct ctcgtaacgc
     1501 aatggctagc cgcaagctgt taaacaagga ttcgctcccg taaggtgcgc cattggcaac
     1561 gatcacgatt ttttgcatta tttactcctg tattcaggga attagacatt catcttctat
     1621 cttactgctt ctgcagcgtc tgaccaatcg gtcacatttt taaggatttt cctgaaagcg
     1681 cgagaaaata cgacaaaagt tgccagtaat cgttattctt taaggctatg gtttttcatt
     1741 attaccggaa gttaccgacg ttttgagccg tttcgttcct cgcattatcc cgttttattt
     1801 actcttgctt gtagcaggcg gtacagctaa cgcacaatct accttcgagc aaaaagcggc
     1861 aaatcccttt gataataaca atgatggtct gccggattta ggcatggctc ccgaaaatca
     1921 tgatggggaa aaacactttg ctgaaattgt gaaagatttc ggcgaaacca gtatgaatga
     1981 taacgggctg gatactggcg agcaggcaaa agctttcgca ttgggaaaag tccgcgacgc
     2041 gcttagtcaa caggttaatc agcacgtaga gtcctggcta tcaccgtggg gaaatgccag
     2101 tgttgacgtc aaagtggata acgaaggaca tttcaccggc agtcgtggaa gctggtttgt
     2161 gccgttacaa gataatgatc gttatctcac ctggagccag cttggtctta ctcagcagga
     2221 tgatgggttg gtgagcaatg tgggcgttgg gcaacgctgg gcgcgcggca actggctggt
     2281 gggttataac actttttatg acaacttgct ggacgaaaat cttcagcgag cgggctttgg
     2341 tgccgaagcg tggggcgaat atttgcgact atcggcaaac ttttatcagc cgtttgctgc
     2401 atggcatgaa cagacagcca cgcaggaaca acggatggcg cgcgggtatg acctgacagc
     2461 ccggatgcgc atgccgttct atcaacacct caataccagt gtcagcgtag aacagtattt
     2521 tggtgatcgt gttgatttgt ttaactctgg tacgggttat cacaatcccg tcgcgttgag
     2581 tctgggatta aattacaccc ctgtgccatt agtcactgtg acggcccagc ataaacaggg
     2641 tgaaagtggc gagaatcaaa ataacctcgg gctgaatctt aattaccgct ttggtgtacc
     2701 gctcaaaaaa caactttctg cgggtgaggt tgccgaaagt cagtcgttac gtggtagtcg
     2761 ctatgacaat ccgcagcgaa ataatctacc gactcttgag taccgacagc gaaaaacgtt
     2821 aacggtgttt ctggcgacac cgccgtggga tctaaaacct ggcgaaacag tgccgctgaa
     2881 attacaaatc cgcagtcgtt acggtattcg gcaactgatt tggcagggcg atacgcagat
     2941 attaagtttg acgccgggcg cacaagccaa cagcgcggag ggctggacgc tgatcatgcc
     3001 tgactggcag aacggggaag gggcaagcaa tcactggcga ttgtcggtgg tggtggaaga
     3061 taaccagggg cagcgtgtct cctccaatga gatcacgcta acgcttgtcg aaccgttcga
     3121 tgcattgtca aacgacgaac tgcgctggga accgtaatca gaaaatgcgc tcctgatgca
     3181 cccataccgc tgcttccacg cgagacttga gcttcatttt cttcagcatg tgcttgacgt
     3241 gcacttttac tgtgctttcg gtgatatcca ggcggcgggc aatcatcttg ttcggcaaac
     3301 cctgggcaat cagcttgaga atatcgcgct cgcgtggggt taactggtta acatcgcgct
     3361 cagtagtggc acggttagcg cgcaagctgg cggccagaac aggcgttaat gcttcgctta
     3421 ataccatttc gccagcagct gcctgatgca atgctttcag cagatcttcc ggttccatat
     3481 cttttaacag ataaccatcc gcgccgcgtt tcagtgcggt gaccacatct tcttcatggt
     3541 tagagacgct gaataccaca atgcgccctg agagggactt ttcgcgcagt ttatccagcg
     3601 tttccagacc gttcatgccg ggcatattga gatctaacag gatcagatcg ggatcaagag
     3661 actccgccag ttcaataccc tgttcgccat tactcgcttc gccaaccacg gtgatatctg
     3721 gtgccatact gataagctgt tttacgccag ttcgcagcat cgggtgatcg tcaatcagca
     3781 ggatagtagc cggttcctga ttactcatgg gtatctcctt ggacgtctgt gaaagttttt
     3841 tcgggaataa aggtgaccac cacttcggtg ccacctgatt cacgacggcg gacgcggcaa
     3901 tcgcctcgta aactttgcgc acggtcgcgc attattatca tgccgtagtg attgctgcgg
     3961 atggcatttt caggcacgcc gcagccgtta tcctggacgg tcagtttgac ctgattatcg
     4021 ttttgcgcca ccgtcaccac gacttcactc gcttgcgagt gtttgagggc gttacttaat
     4081 gcctcacggg caatttgcaa caagtggatt gcctgatgcg aaggcaccag gcgaggcggc
     4141 aattgataat ccagcttcac cgggaagcca aatttggcgc tgtactcttc gcaactcgcc
     4201 tccagcgccg gacgtaatcc aggctcggtg agctgcaagc ggaatgtggt gagcaattca
     4261 cgcaactgcg cccaggatgc attcagttcg ttacggatct gacttaacag ttcgcggctg
     4321 ctttctggca gcgcatcgcc ctgcatctgt aaacaactca cctgcatctt catgcaagag
     4381 agagattggg caatagaatc atgcagttcg cgcgcaatgg tggcacgctc ttccatcacg
     4441 atcaactgtt gctgacgttc ctgatggcga tccagcgcca gcgtggcggt gagttgttca
     4501 accagggtat ccaccagttg ttgttgatca tggctaagat gacgcccctg cggcagggtc
     4561 gccagcaaaa taccgtactg cgtatgagag tcagccagcc gccacttcag ggttgtgccg
     4621 cgatcaccaa cgggtaatac gccgcgcggg cagagctggc agcctttatc atcacaagtc
     4681 atatctggtt ggcaggtaaa ctcctgatga ttctcttcat catcagtatc ataaacccgc
     4741 agttcgatat cacgtagcag ggttaaattc tgtaagccgt tgagtacagg tgacaggcgt
     4801 tcacacagcg gggcttggga atgcaaacgg cggttagcct gccataaaaa agagaggatc
     4861 tgatttttat gctccagccc ggcggttttc tcctgaaccc gctgctcaag tacggcataa
     4921 ctttcggcca gttctgcaga catattgttc aacgcagttc caagcatcgc catttcgttg
     4981 cgcccgctga tgtttgcgcg ttgggtaaaa tcgcgatgac tgacggcact cgccattgcc
     5041 agcagttgcc gccacggttg tagcagtcgc gcccgcaacc agataatagt gaacaccagt
     5101 aaaagtgcca taaataccgc cattacccga tggaccagta ccactgtctc gatgcgcatt
     5161 tccgtggtgc ggtcaaaacc agataccagt tgatcaagcc cggcaacaaa ctggctgaca
     5221 tccgctgaca ccgtttctcg gttttgtgca cgcatcaacg cagggatcag ttcattacgc
     5281 cagtaatctt gtaaaccctg taattgcgcc agttgtccgt ctcgttctgc tgctcgagtc
     5341 aactcggcgc taaatgccgt ttgttccatc tctttaatta agggcttgtc tttctcgctt
     5401 aatggcactg ctgccaacag acggtaactt tgcatgcgca gcgatcccgc tttgttgatc
     5461 gcatgggcgc tgccctgaac gccttgcacc agccagccag aaaccgccat ccctgccagt
     5521 ccaatagcag tagaaagcaa cacaataagc gcaacctgat taaccagggt gagcggagag
     5581 agacaacgtt taagcatgta aacctcttcc ttcaggcttt aaatgagcaa taaccttaat
     5641 gaatgtgacg atacattctg gaatggcagt attctcggct attggctgaa gtatacccat
     5701 acctggaaag agttactcct tatttgccgt gtggttagtc gctttacatc gttaagggta
     5761 gggattctac agcaccgtga aaaatctcat aatttttatg aaatcactgt actcactatg
     5821 ggtaatgata aatatcaatg atagataaag ttatcttatc gtttgattta catcaaattg
     5881 cctttagcta cagacactaa ggtggcagac atcgaaacga gtatcagagg tgtctatgag
     5941 tcactcatcc gcccccgaaa gggctactgg agctgtcatt acagattggc gaccggaaga
     6001 tcctgcgttc tggcaacaac gcggtcaacg tattgccagc cgcaacctgt ggatttccgt
     6061 tccctgtctg ctgctggcgt tttgcgtatg gatgttgttc agcgctgttg cggtgaacct
     6121 accgaaagtc ggctttaatt ttacgaccga tcagctattt atgttgactg cgctgccttc
     6181 ggtttctggc gcgttattac gtgttccata ctcctttatg gttcctatct tcggtggtcg
     6241 tcgctggacg gcgttcagca ccggtattct gattattcct tgcgtctggc tgggttttgc
     6301 cgtgcaggat acctccacgc cttatagcgt cttcatcatc atctctctgc tatgcggctt
     6361 tgctggcgcg aacttcgcct ccagtatggc aaacatcagc ttcttctttc cgaaacagaa
     6421 gcagggtggc gcgctgggtc tgaatggtgg tctgggcaac atgggcgtca gcgtcatgca
     6481 gttggttgct ccgctggtgg tatcactgtc gattttcgca gtatttggta gccagggcgt
     6541 caaacagccg gatgggactg agctgtatct ggcgaatgcg tcctggatat gggtgccgtt
     6601 ccttgccatc ttcactattg cggcgtggtt tggcatgaac gatcttgcta cctcgaaagc
     6661 ctccatcaag gagcagttgc cggtactcaa acggggtcat ctgtggatta tgagcctgct
     6721 gtatctggca accttcggct ccttcatcgg cttctccgcg ggctttgcaa tgctgtcaaa
     6781 aacgcagttc ccggatgttc agattctgca atacgctttc ttcgggccgt ttattggtgc
     6841 gctggcgcgt tctgcaggtg gtgcattatc tgaccgtctg ggcggaactc gtgtcacgct
     6901 ggtgaacttt attctgatgg cgattttcag cggcctgctg ttcctgacct taccgactga
     6961 cgggcagggc ggaagcttca tggcgttctt cgcggtcttc ctggcgctgt tcctgacagc
     7021 tgggctgggt agtggttcca ctttccagat gatttcagtg atcttccgta aactgacaat
     7081 ggatcgcgtg aaagcagaag ggggttctga cgaacgtgcg atgcgtgaag cggcaaccga
     7141 cacggcagcg gcgctgggtt tcatctctgc gattggcgct attggtggct tctttatccc
     7201 gaaagcgttt ggtagctcgc tggcattaac gggttcgcca gtcggcgcaa tgaaggtatt
     7261 tttgattttc tatatcgcct gcgtagtgat tacctgggcg gtatatggtc ggcattctaa
     7321 aaaataaacc gttactcgtc atacttcggg ttacatgtgc tgcggctgcg ttcattcacc
     7381 ccagtcactt actttagtaa gctcctggga ttcattcact tgccgccttc ctgtaaaccg
     7441 aattatatag agtaaaatat ttgattatcc tttgcgcggc atg
//
LOCUS       DAZFRJ010000195         7470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000195 DAZFRJ010000000
VERSION     DAZFRJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.197"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(139..843)
                     /locus_tag="ACHKUN_003962"
     CDS             complement(139..843)
                     /locus_tag="ACHKUN_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6662125.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(980..1432)
                     /gene="moaE"
                     /locus_tag="ACHKUN_003963"
     CDS             complement(980..1432)
                     /gene="moaE"
                     /locus_tag="ACHKUN_003963"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO6662126.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1434..1679)
                     /gene="moaD"
                     /locus_tag="ACHKUN_003964"
     CDS             complement(1434..1679)
                     /gene="moaD"
                     /locus_tag="ACHKUN_003964"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO6662127.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1672..2157)
                     /gene="moaC"
                     /locus_tag="ACHKUN_003965"
     CDS             complement(1672..2157)
                     /gene="moaC"
                     /locus_tag="ACHKUN_003965"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO6662128.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVTALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2160..2672)
                     /gene="moaB"
                     /locus_tag="ACHKUN_003966"
     CDS             complement(2160..2672)
                     /gene="moaB"
                     /locus_tag="ACHKUN_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO6662129.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2694..3683)
                     /gene="moaA"
                     /locus_tag="ACHKUN_003967"
     CDS             complement(2694..3683)
                     /gene="moaA"
                     /locus_tag="ACHKUN_003967"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO6662130.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3671..3815)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4080..4988
                     /gene="yvcK"
                     /locus_tag="ACHKUN_003968"
     CDS             4080..4988
                     /gene="yvcK"
                     /locus_tag="ACHKUN_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO6662131.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5180..7201)
                     /gene="uvrB"
                     /locus_tag="ACHKUN_003969"
     CDS             complement(5180..7201)
                     /gene="uvrB"
                     /locus_tag="ACHKUN_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO6662132.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1797 a         1980 c         1876 g         1817 t
ORIGIN      
        1 aatacttttg tatttatgga tacggaaaac gcttccgctc gccataaggc gtattttata
       61 cttggtactt gttaagtgtc gtttttagta agaaaaaaaa tcccgacaca tggccgggat
      121 attgatggtg aaaaagaatt aacggcggtt gccaaaaatt cgcagcaaca tcaggaacag
      181 gttgatgaag tccagataca aggttaacgc gccaagaatg gaatatttgc gcaggttcga
      241 cgtgtcgcgg gtatcaatct gctcacccat atttttcaat ttctgagtgt catacgccgt
      301 caatccgaca aagacaatca cgccgatgta ggtaactgcc cacatcaatg cttcgctttt
      361 cagccagaag ttgaccagcg atgccagcac aatgccgatt aacgccataa acagcatatt
      421 gccaaagcca cttaaatcgc gcttcgtggt gtaaccgtac aggctcattg cgccgaacat
      481 cccggcagta acgacgaaag tactggcgat agaagcagcg gtatagacaa tgaatatact
      541 ggaaagcgta agacccgtca gcgccgaata aagcataaag agcatcgtcg ttacacctgc
      601 gctcagcttt tgaatcatcg ctgataacac aataaccaat gctaattgcg cgatgatcag
      661 accgattaaa aagacacggt tagtaaacaa cagctccatc acggccgcgg aattagccgc
      721 ataccaggca acaaatgcgg tcagcaacaa gccaacggtc atccagccat agacttgcgc
      781 catataagtt tgcaagccag cccggggttg tacgattgaa tcagaacgtg ggaatctgtc
      841 catgacgatc tcctgaagat ataaggaata tcttaaggat actgcaaaat gatgaggctg
      901 tgcatcgacg cagcgtaaac gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc
      961 ttatccggct aacaaaaaac taccagcgtt ttgccgcctg ctgatcgctc tcccgagctt
     1021 caacccagcg gtcgccttcc ggcgtggctt cgcgcttcca gaacggtgcg cgggttttga
     1081 gataatccat aataaactgc ccggcttcaa acgcactgct gcgatgcgca ctggtgacac
     1141 cgacaaaaac gatttcatcg cccggccata attccccgat gcggtgaatc acagtgacgc
     1201 gccccagcgg ccagcggtta cgcgcttcat caacaatttc tgccagtgct ttttcagtca
     1261 tccccggata gtgttcgagg gttaatgctt tgacgctgtc gcccaggtta tggttgcgca
     1321 ccttaccagt aaaggtgact accgcaccgt cttcgtcacg ctccgccagc cacgggtact
     1381 cttctcctac gctgaacggc tgcggaccaa caacaatttt ggtttctgcc atcttaacct
     1441 ccggttaccg gcgggaagaa agctacttcg tcgccgtcag tcagcggatg gtcaaaactc
     1501 accagcgtct ggttgacggc agccagtaat ttgccatctt ccagcgccag cgcccagcga
     1561 tcgctctgcg cagccatgtg ctggcgtaac gcttcaacag ttgggaaatc cgcagccact
     1621 tcggttgcat ctgttcccac caactcgcgc acctgggcga aaaaaagaac tttaatcatc
     1681 cgcttccacc ttaaagtcac ccgacttgcc gccgctcttc gccagcaaac gtaccggacc
     1741 aatcaccata tctttttgca ccgctttgca catgtcataa atggtcagcg ccgtcacgga
     1801 ggccgcggtt aatgcttcca tttcgacacc ggttttcccg gtcaggcggc ataaggtttc
     1861 tatacgcacc cgattgtgct ccggctcggc ctgtaaattg acttcaactt tgctgagcat
     1921 cagcggatga cagagcggga tcagatccca ggtgcgtttt gccgcctgaa taccggcaat
     1981 acgcgcagtg gcaaatacgt cgcctttgtg gtggcgacca tcaataatca tcgccagcgt
     2041 ctcgctgcgc atggtgacaa aggcttccgc ccgcgcttca cgcacggttt ccgctttggc
     2101 ggagacatcc accatgtgcg cttcgccagc ggcgttgata tgggtcagtt gcgacatact
     2161 tatttcttca aatgtggatg gaaattacac ggacgcgtac gggcatccag ctgcggcgcg
     2221 atgatatttt cccatgcggt acggcacgct ttggtcgaac ccggcatggc gaaaatcagc
     2281 gttttgttgg cgacgcccgc taccgcacga gattgcaacg tggaagtgcc aatctcttca
     2341 aacgacaaca tacggaacac ttcaccaaaa ccttcaattt cacggtcgaa caacggcagc
     2401 aatgcttcgg gagcctgatc accttccgtc aggccagtac cacccgtaat caataccact
     2461 tgtacatcgt cgctggcgat ccacgcagat acctgagcgc gaatagcgta gcggttttct
     2521 ttcacaatgg ctttatcgac aacgtgatgg cccgcttctt gcgccgaatc gcgcagatag
     2581 tgaccggagg tatcgtcttc ttcaccgcga cgattagaaa ccgtaagaat agcaatacgg
     2641 gtcgggataa attcagtgct tacctgactc atctgatctc tccttttgac gttttagccg
     2701 ccaatgtacg ataagttttg cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt
     2761 ttcttctccc gcagcgccgc tgaaatacgc gcttccagcg cctgttgctg ggtatcgtct
     2821 tccagcagat cgcgcaggtt aacgccgcct tcaccaaaca ggcagagatg gagtttacca
     2881 atggaggaaa cgcgcaggcg gttgcaagtg gcgcagaagt ctttttcata cggcatgata
     2941 aggccaatct ctccggcgta atctggatgg caaaagactt gcgcgggacc gtcgctgcgt
     3001 tgacgtaatt ggtggatcca gccgcgacgc agtagctcgt cacgcagaac ctgaccagag
     3061 atgtgatgct tacggaagag ctcgctgccc tcgcccgttt ccatcagttc gatgaaacgc
     3121 agctggatag ggcgatgctg gatccagttc agaaaggtgt cgagctggtg atgattaaca
     3181 tcacgcatca gcacggtatt gactttgacc ttctcaaaac cggcctcaaa tgcagcatca
     3241 atccccgcca tgacctggtt gaatttatcc tgcccggtaa tagcgtgaaa ctggcgggcg
     3301 tccagactgt cgacactgac gttaatgcca gtaagtcccg catcgcgcca gttcgccaca
     3361 tcgcgttcca gacggtaacc attggtggtg accgcaatct ggcggatagc gtcgttttcc
     3421 cgcacagcgg cgatgatatc ggtaaagtcg cggcgtaaag acggctcacc gcctgtcagg
     3481 cgcacttttt cggtgcccag actggcgaag gcgcgcgtaa cccggcgaat ttcatcgacg
     3541 gtaagaaagc ctttattggt gacgccgctc ggtttgtagc catccggcag gcagtaggtg
     3601 caacgaaagt tacacacatc ggtaatcgac aggcgcaagt agtaaaactt acgcgcaaat
     3661 gcatcagtca gttgtgaagc catgtacacc tttccagata cgggaggcga agtcatttct
     3721 tccttcgccc tggtggcaat ctttacgagc attgtcacgg ccaaagcacc gtatcagttg
     3781 acccaggtgc agaggctaga gtgtttagtg gttatgccga tactagcgtg taaatgagat
     3841 ttttaccatc cacactttcg ctatgtaatc atgtatatag cgtcatgatc gtgctatttc
     3901 gctacagaat aagggaaaat ggcggtttta ctttgatata catcatcagg gaggcatagc
     3961 gtgcatcgtc ctttagaggt agaagaaaat agtttgtatc ctcaatattg gcaggttaat
     4021 tgctgtttcc ccgcaatttg cgctactgta gcgcggataa tctgactcca ggaaactata
     4081 tgcgcaatcg tacgctggct gatcttgatc gtgtcgttgc tctcggcgga gggcatggac
     4141 tgggacgcgt tctctcatca ctttcgtctt tgggttctcg tttaacgggt atcgtcacca
     4201 ccaccgataa tggtggctcg acggggcgta ttcgccgttc agaaggcggc attgcctggg
     4261 gcgatatgcg caactgcctc aaccagctga taacggaacc gagcgtcgcc tccgcgatgt
     4321 ttgaataccg ttttggtggc aatggcgaac tttccggtca taatctcgga aacttgatgt
     4381 taaaggcgct ggatcacctt agcgtgcggc ctctggaagc catcaattta attcgtaatc
     4441 tgctgaaagt ggatacgcat ttgattccaa tgtcagagca tcctgttgat ctgatggcga
     4501 ttgacgatca ggggcatgaa gtttacggcg aggtcaatat cgaccagtta actacgccga
     4561 ttcaagagtt attgttaacg cctaatgtac ccgcaacgcg tgaggcggtt cacgctatca
     4621 atgaagcgga tctcatcatt attgggcctg gcagttttta taccagcctg atgccaattc
     4681 tgctgctgaa ggaaatcgcc caggcattac gccgcacgcc agcgccgatg gtttatatcg
     4741 gcaatctggg gcgtgagttg agtttacctg cggctaattt gaagctggaa agcaagctgg
     4801 caattatgga gcagtatgtt ggtaaaaagg tcattgatgc ggtcatcgtc gggccaaaag
     4861 tggatgtctc ggcggtgaaa gagcggattg tgatccagga ggtactggag gccagcgata
     4921 ttccgtatcg tcatgaccgc cagttgttac ataacgcgct ggaaaaggcg ttacaggctt
     4981 taggttaacg gaaatccgaa ggaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg
     5041 tttatgccgg atgcggcgtg aacgccttat ccggcctaca aaccgcgcaa attcaatata
     5101 ttgcggagaa aatgtaggcc tgataagcgt agcgcatcag gctgttttcc gtttgtcatc
     5161 agtcttcttc gctatcctgt tacgatgccg cgataaacag ctcacgcagc tgatgcaact
     5221 ggtcacgaat ttgcgccgct tcttcgaact ccagattctg cgcgtgttgc atcatcaacc
     5281 cttccagctc atggattttc tgctgcaacg ctttaggcga catatccatc ggcacattat
     5341 ccggctcaac aatcggtcgc gattttcctc tgcccttcgc tttggtttta gcaatgttct
     5401 gccccaacgc caggatatcg accactttct tgttcaagcc ttgcggcgta atgccgtgtt
     5461 cctcgttgta cttctgctgt ttctcgcggc gacgttcggt ttcgccaatc gctttcgcca
     5521 ttgatggggt gatcttatcg ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac
     5581 gaccaatggt ctggatcaac gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga
     5641 ggatcgccac cagcgaaact tccggcatat ccagaccttc acgcagtaag ttgatcccta
     5701 ccaatacgtc gaactcaccc agacgcaagt cgcggataat ctccatacgc tcgacggtgt
     5761 cgatatctga gtgaagataa cgcacgcgct caccgtgttc ttcgagatat tcggtaagat
     5821 cttccgccat tcgcttggtc agtgtggtga ccagtacgcg ttcgttaatt gccgctcgct
     5881 gacgaatctc cgaaagaaga tcatcaacct gtgtcgccac cggccgcact tcgataatcg
     5941 ggtcaagcaa tccggttgga cgcaccacct gatccaccac atcgccgccg gatttttcca
     6001 gctcgtaatt acccggcgtc gccgaaacat agatggtttg cggcgctaat gcttcgaact
     6061 cttcaaactt aagcggacgg ttatccagcg ctgatggcag gcggaagccg tactccacca
     6121 gtgtctcttt acgcgcccgg tcaccgcgat acatgccgcc aatttgtgga atggtgacgt
     6181 gagattcatc gaccaccagc agcccatcag cagggaggta atcaaacagc gtcggcggtg
     6241 gctcacccgg tccacgaccg gagaggaagc gcgagtagtt ttcaatcccc gaacagtagc
     6301 ccagctcgtt catcatctcc agatcaaact gggtacgctg ggtcagccgc tgctcttcca
     6361 gcagtttgtt gttttccaac agcactttgc gtctggcggc cagctcttct ttgatctcct
     6421 ccatcgcctg tacgatgcgc tcgcgcggtg tgacgtagtg cgttttcggg tagatggtaa
     6481 aacgtggaat agtggaaaca atctgcccgg tcagcgggtc aaataacgac aatcgttcca
     6541 cttcctcgtc aaacagttcc acgcgaagtg caatgtcatc cgattctgcc gggaagatat
     6601 ctatcacctc gccacgaacg cggaaagtac cacgctggaa tgcttgatca ttacgagcgt
     6661 attgcagctc cgccagtcgg cgcagaatcg cgcgctgatc gataatcata ccgaccgtga
     6721 gatggagcat catcttgaga tataaatcag gatcgcccag accataaatc gcggaaacag
     6781 acgccaccac aaccacatca cgccgctcca gcatcgcttt ggtggcggac aaacgcatct
     6841 gctcaatatg ttcgttaacc gaggcatctt tctcaatgaa agtgtcggaa ctcggtacat
     6901 aggcttccgg ctgatagtag tcgtagtagg aaacgaaata ttccaccgcg ttttccggga
     6961 agaactcttt catttcgcca tacagctggg ccgccagcgt tttgttgggc gcaagtacca
     7021 tggttgggcg ctgaaggtca gcaatgacat tggcaatggt gaatgttttc ccggagccag
     7081 tcacgccaag taacgtctgg tgcgccaggc catcttccag cccctcttcg agacgtcgaa
     7141 tcgcctctgg ctgatcgcca gaaggtttaa aagcggaatt cagtttgaac ggtttactca
     7201 tgagtcgcta cctgaaggag ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat
     7261 tatgccaaca aattatactg gataaaaaaa cagttcatca ccataatatt tctgttacag
     7321 cgtaaactcc gctgtcaatc atgagcaaaa tttactctgt ggcgagataa aactccggcc
     7381 ttaccgggtt atccccaaag caacggcttt tttaacattt gtcaagatga gtgatgacag
     7441 ttttatggca agagatgcct gttcagtgac
//
LOCUS       DAZFRJ010000196         7452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000196 DAZFRJ010000000
VERSION     DAZFRJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.198"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..373
                     /gene="ecpR"
                     /locus_tag="ACHKUN_003970"
                     /gene_synonym="matA"
     CDS             <1..373
                     /gene="ecpR"
                     /locus_tag="ACHKUN_003970"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO6662133.1"
                     /translation="FINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYA
                     GLSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKT
                     VYTHRRNAEAKLYSKIYKLVQ"
     gene            447..1034
                     /gene="ecpA"
                     /locus_tag="ACHKUN_003971"
                     /gene_synonym="matB"
     CDS             447..1034
                     /gene="ecpA"
                     /locus_tag="ACHKUN_003971"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO6662134.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1092..1760
                     /gene="ecpB"
                     /locus_tag="ACHKUN_003972"
                     /gene_synonym="yagY"
     CDS             1092..1760
                     /gene="ecpB"
                     /locus_tag="ACHKUN_003972"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO6662135.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1786..4311
                     /gene="ecpC"
                     /locus_tag="ACHKUN_003973"
                     /gene_synonym="yagX"
     CDS             1786..4311
                     /gene="ecpC"
                     /locus_tag="ACHKUN_003973"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO6662136.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4301..5944
                     /gene="ecpD"
                     /locus_tag="ACHKUN_003974"
     CDS             4301..5944
                     /gene="ecpD"
                     /locus_tag="ACHKUN_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO6662137.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5913..6623
                     /gene="ecpE"
                     /locus_tag="ACHKUN_003975"
     CDS             5913..6623
                     /gene="ecpE"
                     /locus_tag="ACHKUN_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO6662138.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6936..7265
                     /locus_tag="ACHKUN_003976"
     CDS             6936..7265
                     /locus_tag="ACHKUN_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6662139.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7260..>7452)
                     /locus_tag="ACHKUN_003977"
     CDS             complement(7260..>7452)
                     /locus_tag="ACHKUN_003977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662140.1"
                     /translation="FYILIQIVSEYIALLQDDVDPKNATEERHCCWQIEEVSGFVTPE
                     KRSPAIIAGGAVQLIIVCL"
BASE COUNT         1913 a         1935 c         1950 g         1654 t
ORIGIN      
        1 atttataaac cgtgataatg aatggttatc cgcggtaaag gggaaacagg tcgtattgat
       61 tgcggccaga aagtcagaag ccttagcaaa ttattggtat tacaacagca atattagggg
      121 cgtggtatac gcaggactga gtcgtgatat tagaaaagaa ctggcctatg tgattaatgg
      181 caggttcctg agaaaagata ttaagaaaga taaaatcacg gaccgggaaa tggaaattat
      241 ccgcatgacg gcccagggaa tgcaacctaa atcgattgcc agaattgaaa attgtagtgt
      301 gaagacagtg tatacccatc ggcgtaatgc tgaggccaag ctgtactcaa aaatatataa
      361 gttggttcag taaactccag gctagttagt tttaaaaatg actcactggg acatcacgtc
      421 ctcaattcaa ctcgggaaga aatgcaatga aaaaaaaggt tctggcaata gctctggtaa
      481 cggtgtttac cggtataggt gtggcgcagg ctgctgacgt aacagctcag gctgtagcga
      541 cctggtcagc aacagccaaa aaagacacca ccagtaagct ggttgtgacg ccactcggta
      601 gcctagcgtt ccagtatgcc gaaggcatta aaggttttaa ctcacagaaa ggtctatttg
      661 acgtggctat cgagggtgac tcaacggcta ccgcctttaa actgacctca cgtcttatca
      721 ccaacacatt aacccagttg gatacctcag gttccacact gaatgtgggc gtggattata
      781 acggcgcggc agtcgaaaaa actggcgata ccgtgatgat cgataccgcc aacggcgtac
      841 tgggcggcaa ccttagcccg ctggcaaacg gttacaatgc cagcaatcgt accaccgcac
      901 aggatggttt cactttctcc atcatcagcg gcaccaccaa tggtaccacc gcagtaaccg
      961 attacagcac tctaccggaa ggcatctgga gcggcgacgt tagcgtacag ttcgacgcga
     1021 cctggaccag ttaatctctc tgatgtacca gcaggggtag cccccctgct ttattcctgg
     1081 acggaattgt tatgaaaaag caccttctgc ctctcgctct gctgttttcc ggaatatctc
     1141 cggcccaggc gctggatgtc ggcgatatat catcgtttat gaacagtgac agcagcacgc
     1201 tgagcaaaac gatcaaaaac agtaccgaca gtggtcgcct tatcaatatc cgtctcgaac
     1261 ggctctcttc accgcttgac gacgggcagg ttatctcaat ggacaagccg gatgagttgc
     1321 tactcactcc cgccagcttg ctgctacccg cccaagccag cgaagtgatc cgcttcttct
     1381 ataagggacc cgcagatgaa aaagagcgct actaccgcat tgtctggttt gatcaggccc
     1441 tcagtgatgc gcagcgcgat aatgccaacc gcagcgctgt ggccactgct tccgcccgca
     1501 tcggcaccat tctggtcgtc gcccctcgtc aggcgaacta ccactttcag tacgccaacg
     1561 gctccctgac aaatacagga aatgcgacgc tgcggatcct cgcttacggc ccctgcctga
     1621 aagccgccaa tggtaaggag tgtaaagaga attactacct gatgccaggc aagtcgcgtc
     1681 gttttacccg cgtggacacg gcggataaca aaggacgggt tgcactttgg cagggtgata
     1741 agttcattcc cgtgaaatag atagctgtgc agatggataa cgacaatgcc tttacgacgg
     1801 ttctccccag gactgaaagc ccagtttgcc ttcggcatgg tctttttgtt cgttcaaccc
     1861 gatgccagcg ctgctgacat aagtgcgcag caaataggtg gggtgattat tccgcaggcc
     1921 ttcagtcagg cgcttcagga cggcatgagc gtcccgctct atattcatct cgccggtagc
     1981 cagggtcgcc aggacgatca gcgaatcggc agcgctttta tctggctgga tgatggacag
     2041 ctacgcatcc ggaaaataca gctggaagag agtgaagata acgccagtgt cagcgaacaa
     2101 actcgacagc agctgatggc tctggcgaac gccccgttca atgaggccct taccatcccc
     2161 ctgactgaca acgcgcagct ggatctcagc ttgcgccaac tgctgctgca gctggtggtc
     2221 aagcgcgaag cgctgggcac cgtactacgc tcacgtagcg aagacatcgg gcagtccagt
     2281 gttaacaccc tcagcagtaa tctgagctat aacttgggcg tctataacaa ccagttgcgt
     2341 aacggcggga gcaacacatc cagctatctg tcgctgaata acgttactgc gctgcgcgaa
     2401 catcatgtgg tgctcgacgg ctcgctgtac gggatcggta gcggtcaaca ggacagtgaa
     2461 ttatataaag cgatgtatga acgcgatttt gccggtcacc gatttgccgg tggaatgctc
     2521 gacacctgga acttgcagtc cttagggccg atgaccgcca tttcagcagg gaagatttac
     2581 ggcctttcct ggggaaacca ggccagctcc accatcttcg acagcagcca gtcagccacg
     2641 ccagtgatcg cctttttacc ggcggcgggc gaagtacatc tcacccgtga tgggcggcta
     2701 ctaagcgttc agaacttcac tatgggcaat catgaagtgg atacccgggg tctaccgtac
     2761 gggatttacg atgtggaagt tgaggtgatc gttaacggtc gcgtgatcag caaacgcccc
     2821 cagcgggtca ataagctgtt tagccggggg cgcggcgtcg gtgcaccact ggcgtggcag
     2881 gtatggggcg gtagctttca tatggatcgc tggtcggaaa acgggaaaaa gacgcgacca
     2941 gctaaagaga gttggctagc aggtgcctcg acctccggct cattgagtac gcttagctgg
     3001 gcggcaacgg gatatggata cgataatcag gcggtgggtg aaacccgtct gacgctgccg
     3061 cttggggggg cgatcaacgt taacctgcaa aatatgctgg ccagtgacag ctcatggagc
     3121 agcatcggca gcatcagcgc cactctaccg ggaggcttta gttcgctgtg ggttaaccag
     3181 gaaaaaaccc gcattggcaa tcaattgcga cgtagcgatg ccgacaaccg tgcaatcggc
     3241 ggcacactca acctgaactc actgtggtcg aagctgggca cattcagcat cagctacaat
     3301 gatgaccgcc gttacaacag ccattattac acggcagatt actatcaaaa tgtctacagc
     3361 ggtacctttg gttcgcttgg cctgcgggcc ggtattcagc gctataacaa cggcgacagc
     3421 aacgccaata cagggaaata tatcgctctc gatctctcgc taccactggg caactggttt
     3481 agcgcaggga tgactcatca aaacggctac accatggcaa acctgtcagc acgcaaacag
     3541 tttgatgaag gaaccattcg cactgttggt gccaatctgt cacgtgccat ctccggcgat
     3601 accggtgatg acaaaaccct cagcggtggg gcgtatgcac agttcgacgc tcgttacgcc
     3661 agcggaacgc tgaacgtcaa tagcgcggcg gacggctaca tcaataccaa cttgaccgcc
     3721 aatggcagcg tcggctggca gggtaaaaac atcgctgcca gcgggcggac tgatggcaac
     3781 gctggggtga tattcaacac cgggctggag gacgacggtc agatcagcgc caaaatcaac
     3841 gggcggattt tcccgcttaa cggcaagcgt aactatctcc cgctctctcc ctatggaaga
     3901 tatgaggtgg agttacagaa cagcaaaaac tcactcgaca gctacgatat cgtcagcggc
     3961 cgcaaaagcc atctgactct ctatccaggc aatgtcgctg tcattgagcc agaggtgaag
     4021 cagatggtta ccgtctccgg tcgtatccgt gcggaagacg gcacactgct ggctaacgca
     4081 cggattaaca accatatcgg ccgaacccga accgatgaaa acggcgagtt tgtcatggac
     4141 gtggataaga aatatcccac tatcgatttt cgctacagtg gcaataaaac ctgcgaagtg
     4201 gcactggaac tcaaccaggc gcgcggtgcc gtctgggtcg gtgatgtggt ctgcagcggc
     4261 ctctcatcgt gggcggaggt gacgcagaca ggagaagaga atgagagtta acctactaat
     4321 aacgatgata attttcgcgc taatctggcc agtaactgag ctcagagcgg cagtgagcaa
     4381 aacaacctgg gcggatgcac cggcacgcga gtttgtgttt gtcgaaaaca actcagacga
     4441 caactttttc gtcactcctg gcggggcgct ggatccgcgc ctgaccggtg ccaaccgctg
     4501 gaccggttta aaatacactg gttcaggaac catctatcag caaagcctcg gctacattga
     4561 taacggttac aacaccggcc tttataccaa ctggaagttt gatatgtggc tggaaaattc
     4621 accagtttca tctcctttaa ctggcttgcg ctgcatcaac tggtacgctg ggtgtaatat
     4681 gaccaccagt cttatcctgc cgcaaaccac cgacgccagt ggattttatg gcgcgacggt
     4741 gaccagcggc ggcgcgaagt ggatgcacgg catgttgtca gacgcgtttt accagtatct
     4801 gcaacaaatg cccgtcggca gcagctttac aatgaccatc aatgcctgcc agacctctgt
     4861 gaactatgac gccagcagcg gcgcacgctg taaggatcag gcctccggca actggtatgt
     4921 tcgcaacgtc acccatacga aagcagcaaa tctgcggtta ataaataccc actcgctggc
     4981 ggaggtattt atcaacagcg acggagtacc gactctgggc gaagggaacg ccgactgccg
     5041 gacacagacc atcggcagcc tttcaggatt aagttgtaag atggtcaact ataccctgca
     5101 aacaaacgga ctcagcaaca cctcaatcca tatattcccg gcgatcgcca actcgtcgtt
     5161 agcctcggcc gtcggggcgt acgatatgca gttcagtctg aatggcagtt catggaaacc
     5221 ggtgagcaat accgcctatt actacacctt caacgagatg aagagcgcag actcgatcta
     5281 tgttttcttc tcgagcaact tctttaagca gatggtgaac ctcgggatca gcgatatcaa
     5341 caccaaagat ctattcaact ttcgctttca gaacaccaca tcaccggagt ctggctggta
     5401 tgaattttct acctccaaca cgctgattat caaaccccgt gatttcagca tcagtattat
     5461 ctccgatgaa tatactcaga caccgtcgcg ggagggatat gttggcagcg gcgagtcggc
     5521 actcgatttc ggctatatcg taaccaccag cggtaaaaca gctgccgacg aagtgctgat
     5581 caaggtgacc ggacccgcgc aggtgattgg cgggcgctcc tattgtgtct tcagctccga
     5641 tgacggtaag gcgaaagtac cgttcccggc gacactttcc tttattaccc gcaacggagc
     5701 tacaaaaacc tacgatgccg ggtgcgatga tagctggcgg gatatgaccg atgcgctgtg
     5761 gttgaccaca ccgtggactg atatctctgg cgaagtgggg cagatggata agaccacagt
     5821 caaattttcg attccaatgg ataacgccat ttcgctgcgt acggtagatg ataacggctg
     5881 gtttggcgaa gtcagcgctt caggagagat tcatgttcag gcgacgtggc gtaacattaa
     5941 ctaaggccct gctgacagcg gtctgtatgc tggcggcacc tttgacacag gcgatttcgg
     6001 tcggcaatct gacattttcg ctgccgtccg agactgactt tgtcagcaaa cgtgtagtga
     6061 ataacaacaa aagcgcacgg atataccgta ttgccatcag tgctattgat agcccgggca
     6121 gcagtgaatt gcgcacccga ccggtggatg gtgaactgct ttttgccccc cgccaactgg
     6181 cgttgcaggc tggtgagagc gagtatttta aattttacta tcatggtcca cgggataacc
     6241 gcgagcgcta ctaccgggtc tcatttcgcg aagtccccac tcgtaaccag acaagacgta
     6301 gcccaaccgg cggcgaggtc agcacggagc cggtggtggt gatggatacc attctggtag
     6361 tacgaccacg tcaggttcag tttaaatggt ccttcgacaa ggtgacagga acggtaagta
     6421 acactggcaa cacatggttt aagctactga ttaagccagg atgtgattcg accgaagagg
     6481 aaggcgatgc ctggtatcta cgtccgggag acgtggttca tcagcctgag ttacgtcagc
     6541 cggggaatca ttatctggtc tataacgaca aattcattaa gattagcgat tcctgtccgg
     6601 ctaagccccc ttcggcggac taagacttat ccatcggtcg agggaagaat tccacctcag
     6661 agctccaaaa atcgtttaaa tgatgtggaa gcgatcgtca atgtcacctg taacgatggg
     6721 ctttctcata tgcacaccat agtgttcagg agacacggaa aggtatcagg cagcctgcta
     6781 atgagtggtt taatcacgaa ttagtacgta aaatcggtaa cggctggaaa tcattcaata
     6841 ctcacactat cgaaagttcc ccagccaacc gcggtacgtt cttacatacg atgtaccgct
     6901 gttctcttta cgatttttag ctgtactggt gaattatgag caatctgaat ccatgcatga
     6961 cgtgtggtgc ctgttgtgca tttttccgcg tctcttttta ctgggccgaa gccgacgatg
     7021 ctggcggtac tattcccgcc aggctcactg aacaaatatc cccttttcac cgatgcatga
     7081 gcggtaccaa tcagaaaaac ccccgatgta ttgcccttgc aggaaccccg ggcaaaaatg
     7141 cctgctgcac gatatataaa aatcgatcgt ccacatgcag agaatttgcc atgtctggtg
     7201 agaacggagt cgtcaatgag gcttgcaatc gtgcaagggc taaatacggg ctgacaccgt
     7261 tataaacata caataattaa ttgcactgcc ccgccagcga taatagcggg gcttcgtttt
     7321 tcaggggtaa caaaacccga tacttcttct atttgccagc aacaatgcct ctcttctgta
     7381 gcgttcttcg gatctacatc atcctgaagt agcgcgatat actcactgac tatctgaatc
     7441 agaatataaa aa
//
LOCUS       DAZFRJ010000197         7428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000197 DAZFRJ010000000
VERSION     DAZFRJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.199"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..4786)
                     /locus_tag="ACHKUN_003978"
     CDS             complement(<1..4786)
                     /locus_tag="ACHKUN_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO6662141.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAETGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGK
                     VREALDVHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKST
                     RTQIKKTIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVIL"
     gene            complement(4806..5267)
                     /locus_tag="ACHKUN_003979"
     CDS             complement(4806..5267)
                     /locus_tag="ACHKUN_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO6662142.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5295..7196)
                     /locus_tag="ACHKUN_003980"
     CDS             complement(5295..7196)
                     /locus_tag="ACHKUN_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6662143.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQRQVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVRF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1372 a         2255 c         2003 g         1798 t
ORIGIN      
        1 ccaatataac tttcccacct ttgctttcta tatattctct tactttaggg cttactccat
       61 atttaaacca aaactgagcc tctttcccct gttcttcagc cagctcaatt gtcttcttaa
      121 tttgtgtccg agtagactta cttaagaagt tttttggatt atctatagag ctataagtcc
      181 tcttgacctg cgctatgatc ttgtcagtaa caacatcata ttcacgcccg gcaatttctt
      241 gagatatacc accaagcttt cttcgtacaa gatcctcata atgaacatct aatgcttctc
      301 ttacttttcc ttgtctaaaa agatctcttg ctttatccga aaggcttatt ttttctgcgg
      361 gaacagaagt ttttgccagc cccagcggat caatataccc cagtggatta ggcacatacc
      421 ggtaagtctg ctcacccccc tgcaacccca gcggatcact caccaggtac attccggttt
      481 ccggctcgta gtaccggaac cggttatagc acagccccga ttccgcatcc tgccactgtc
      541 ccgcatacag caggccgggg tcggcttccg ggtcccactc cccgcgcggg tccgggtagc
      601 cggtgtctgc gggcaggctg agtgccagcc cccacaggct ggtctgtccc ggccgccaga
      661 cggttttacc ttcactgtta aagagcatca gcgggcggcc cgtcaggtcg ctcaccgcat
      721 gattcgtccg cgtcacccac tgcggggcca ccgacggatg cgcctgccgt acccggctga
      781 actgctggct tatcagctca aagccgttaa acaccaggtg ccgtacgctg tacagtttat
      841 catcgcggta ttcccggatt tccgcaatac tgtcgccgtc ccacaggtac gtgaagcgga
      901 ttttcttccg gtcgcagcgt ttctctgttc gtctgccgct ggcgtcgtgg cggtattccc
      961 actgctcacc ctgtgcgctg caatacccgg tcagctggtc ccggctgtcc caccggaacc
     1021 gctctgtttc cgggcggtag ccgtcacggt gtctggtgcg gctgaccatc cggcctgcgg
     1081 cgtcatagtc atactgcgtg tttccggcct gtttcagccg gtggcctccg gcatactggt
     1141 cagtctcttc acttatccgg tgcctgcccg cagactgcgc cttcaggtag ccacaactgt
     1201 catagccata ggactcatcg ccttcgccat agcccagccc ggtgcctgag gctgtcaccg
     1261 ccgtgacctg gccgttccgg tccagccggt actcccgccg ggtttcccgt ccgtaatctt
     1321 cccggctggt gacagccgac acattgcccg cgctgtcgta acggtactcc cgtaccagtg
     1381 aggggagcag cccgccccat gtggtttcct ccggaaaaaa tccggcgcgc tgactgacag
     1441 gctggccggc tgcattccag ccctgttcac aggcaaaacc ccggttactg tgccggagca
     1501 cttcacgtcc cgcctcatca cggctgaacg tcagcgtctc atcccccggc agaatgacct
     1561 ctgtcagttc tccggcggcg ttgtagcggt acgtggtttt cagcagggat tcatcggggt
     1621 gtctgtcgcg ttcatcttcc ggtggcagga tacagcggat aacggtgtgt tcatcggggt
     1681 agtggtattc aatggcccgt cggccctgag tggtgacatt cacccgcccg cgttcgtcat
     1741 agccggtgcg ctgtgatgtg ccgtccgggc tgaagatacc cgtcagcctg ccggatttgt
     1801 catacgcata ctgcgttttg ccttccggcg tgatgacttc tgccaggcgg cctgcggcgt
     1861 cccggctgta gaggatggtt tcctccaggc cgttgaccct ggcggtacac aggccatcag
     1921 cgtcatagcg gtaacgccag accacgccgc cccagtcacg ctcttccgtc acccggccac
     1981 agttgtccag aatataacgg tatgactcgc cctgcgggtt aatgacttct gtgagctgca
     2041 gcgtgtcttt atcgtagcgg tactgccagc ggtggccttc accgtccgtt cgcgccaccg
     2101 gcaaatcgaa cgggccgtat tccattgtcc agacaacgcc gttaccgtcc gtgtacgcca
     2161 gcaggttgcc gtggcggtca tagcggaatc cttctgcgcc ggtttctgcc cgctcaaggc
     2221 cgctcagttt atggtgctgc tgccactgta accgcactga ctgaccgccg gggccgcgca
     2281 tcttcaccgg caggtcgtga ccgctgtatt cggtgtggtg ttcacggccc atcacgtcgg
     2341 tgagtgtttc cggccggttg tggtggtcgt actctatgcc cagacgccgt tcatcctggc
     2401 ggaatatccc cgacagcagc ccctgagcgg tgtatgccag ccgggtctct tcgccctgtg
     2461 gcgacaccag gcttaccggc aggccgcgct catcacactg ctgcgtccat gtctgcccgg
     2521 cagggtcggt gatgttgctg agcaggccgc gctcatcata gcggtacagc cactccgcgc
     2581 cgtctgccgc cgtgtaacgg cttaactgac cgagtgcggc gttgtattca aacttctcca
     2641 cccgcccggc aggggagtgg cgggccagca ggtgatggaa ttcatcccat tcatacgtgg
     2701 tggtactgcc gtccggcgct tcttcccgca gaatattgcc gtcaggatca tagtaataac
     2761 ggtgagtgcc cttgccgtcc gtcacggtgg tgcagccgtc accatagtcc agcgtggcgt
     2821 tgtaatagcc ctccgccccg gtgacattca cgcaccggcc ctgtgcatcg taggtgaaac
     2881 ggctccacgt ctggtcgtta tcggaccagc ggatgatccg gtccgcagcg tcgtactcat
     2941 aaaacaggtg gtagtccagc cgcgcatccg cttctgtcag ccgcccccgc gcatcctgtt
     3001 cgtaggtcgc cagcgtctgt atgccgttat ccgtgcggtg aatggctttc aggtagccgc
     3061 tggcgtggta cgtcagttta agccggatcc cgtcggtgtg actgacaccc gtgatccggt
     3121 actggctgtc gcgctcaaac gtgatgcggt tgctggcggt gtcgctcatc ccgcccagca
     3181 tatacatccg gtcgtcgggg ctgtgtgcca ggcggtcatc gctggtatcg tcagtcaccg
     3241 ggtggcgcag acgcattccc ggaaaggcgt cataaaagaa gcggctgctc agtgcatccc
     3301 tgtcgaacag ctccaggtaa tcgtcgcggc ggcgcagaat gtactgggcg tgatacgggt
     3361 taaccgccgt atcactgtca ccgtcgaagg cgaagttcag cgtggctccc tggctgataa
     3421 tatctacccg atttccctgt tcacgcaccc aggcgtattc gctccaggag tcgctccagc
     3481 ccacgccggt caggccatgc agcggcagta cagaactgtg gaaacgggtg aaggagaggg
     3541 ggagggtctg gcccagggtg aagtcggtac gttcgtcgca gtatgcgccg gttaccggat
     3601 cgacaggatc tgttacccat ctgatgactt tattggcaat actttttaac gacttacctg
     3661 ttttccccag caacgcgccg agctttgcgc ccagcagctt cccgcttttc cccagcaggt
     3721 ttcccagttt gccgagtctc gccagattct tcagttttcc cagcagtccc gcaggcggca
     3781 gaagatagct ggctatcgtt attcccccca ggatcattct ctgccacggc gggaactccg
     3841 gctggatatc cagataggtc acctgttcac cgccaataaa gacgtccggc gaacctccca
     3901 cgatcgccgc actgcactcc agtttgtcgc ccttgcgcgc ggcaggctga ttgttgataa
     3961 agacgttgct actgccctgg gctatcctcg gtggtttgtt ctccttgtcg cagagcgccg
     4021 tgtcctcacc ggcttccggg aaatccacat cttttttgat ttcgctacgc ccgcccacca
     4081 tggtggcaaa ctccattaat accggaccgg cgggaaacag aagcgatgcg ccggcgctga
     4141 cgcgatccat aaaaccggca ttgctttccg tcagggtgat aacagcgttc ccccaggcct
     4201 tcgccagtgg tactgctttt tccgctgccg attcaagcag gtcagtggca atatcacccc
     4261 atgagggttc cccctgtggg atttcaggta ttgctccagg tgggggagga gggggaaggg
     4321 tgacggcggc ccttgcagcg ggctttccgt taatgtttac attttcagaa ccggtattga
     4381 tggcgccgtc cgtgtcacaa aagctgttta tccatgattc aagctggttc tgtttctcct
     4441 ccacataatc actcgcctta tagctggcat acatcagcgc aggcaccaga accagcgcat
     4501 aagggccggt ggcgcaggcg gccataatca ggcaaccttc tgcgatggtc actatcgccc
     4561 ccagcacggc ccctgccagt gcccccagaa aacttttatg ctgaatttca tcgcccagcc
     4621 tggcggcagg tgggccggac ttctttgctg ccagcatctg tgcaatggct ttgtcgttgg
     4681 ctgcccgttt catctggctg gcgaccatcg cctgctgtga cagcattcgc attgccagcg
     4741 taccgcctgc ggtcgctccc tgtggcccgc ctggtccttc actcatgtgt tatccctcgt
     4801 ttatgtcagc gcggctggtc cttttggatg tcattctccg gtggcaacgg ggtgaagctc
     4861 tttatcacct ccagcagcat cgtcttttga ctgttcttca gggataagga ggtgctgaaa
     4921 gtgaaggtca gcagcgtatc tccacgccgc tggaatacgg ctatctggct aatccgcccc
     4981 tgattcccct catcttccgg accgttccag gaataatcca gcagccaggc cggggcctct
     5041 gccagagtga tttcccggca ggcatgttcg gtgtacccat caagtttgcc ctggtacagc
     5101 gccagcgttt cctggtacag ctctggtgtc agggttccct gtggaacggc ttcgcgggta
     5161 atgaccaggt tgatgccact gttatctgtg gacaccagga cctgcattga ctgatcctgc
     5221 cattgagacg gcagttgaat gtggccttca ttaaaagtga actgcataaa atactccctg
     5281 ataatctgag tgaatcagtt gatgaaaaca gaaccgccca cgatggtgat gccatccttc
     5341 gacagcctga ttaccgtcga ttccccgacc tgtagctgaa tgccatccgg cgtcagcgca
     5401 attttgcttt cctccacagc cagcaaaatt tccttaatcg ccgtataagc ctgtataccg
     5461 tcgccttcgc caccattgtt tacagcgtca acatgcaccg aaccggcaat cgtgcctctg
     5521 tcctgaaaga catcctcaga taaagaccct ttcagtactc cggtgatctt gtggcctttt
     5581 tccaccgtaa gaaggcggtt accttcctgg atcgtgaccg tctggtcatt ttttacggtt
     5641 tcgcggtgat cgtgttttac ggtggtggta cggtcgttga gcacttcggt atccatgttc
     5701 ttctgcgcgt ggatgtagac ctgttctctc cctgtcgcat catcaaactt cagctcatta
     5761 aatccgctgc ccatgtacgt tttggagcgg atggtcatct gcgtctttgt tcccggcagg
     5821 ctgccggggg tgcggttttc ctggtggtag gtgcgcccca taatgatcgg ctggtccgga
     5881 tcgccgttga ggaagtctac aatcacctcc tgacccacac gcggtatcgc caggtggcca
     5941 aaaccggtgc ctgcccacgc ctgtgccaca cggatccagc acgaactgtc ttggtctgcc
     6001 gggttataac ggtcccagtt gaacctcacc cgcacgcgac catgttcgtc gcagaagatt
     6061 tcctcgcctg ccggtcctgt cacgacagcg ctctgcgggc cgtcgaccag cggtttcagc
     6121 aacggctgtg gtcgccatgt tctgtctgcc gggatcaccg caaaatggtt ctccagcgcc
     6181 gtccccgctc cctgccgtcc tggcaccgcc tgtggctgtt cgccgtgcag ttcacttgcc
     6241 accacctgcc attcccggtt taggttcgcc tgcggatgcc ccgtcagcac aattcgccgt
     6301 cccggccata tctccggcga gcggctcatt ccccgcgcgg tttctgcgtt gtttcgccag
     6361 ccgtccatct gccagcgggc aaagttctgc ccgtgggcgc tcttgaaacg tccggggtag
     6421 tcatacactt catactgcgt gcgctggtag tcctggtgct ggccttcctg ttcaaaacgt
     6481 ccggcccagc cggggcgttt aaaggtgtag tctttggtca ccacggaaga agggcggatt
     6541 tgtgcgctgt agcggaactg gctgatgcag agggtgctca cctcggtacg ggtgttcggg
     6601 ttccatggga tttcaaaaga ttcgggcaga tggcggactg tgtcgcacag caccaggctc
     6661 tggtcggtac ttttgtaagc atgctcctca taaaagaaga tgccttcctc cgccgccatc
     6721 cggcacagaa aatcgtaatc agtctcaccg tactggacac aaaactcacg ggaaggatgc
     6781 ggctcgctga acagcggact ccattcggtc accccgtttt cctgcaacat cgtgccgagg
     6841 atgcttttga tgtcctcgtt ctggaagata cggaagttct ggcgcagacc ggcacgccac
     6901 agcggcgggt gcaccttcat gctgtacagc atctggtttt tgtcgttctc cccgagttca
     6961 aaccaggtca ccacgccttt cacctggcgc tgtaattcat cgccctgcca tatcgtcagg
     7021 taggccattt tgtccagcac ctgcgcaaat tcaagggaga gaaactgctg gctgaccagg
     7081 gagagatcga gggaaaagag cgaagagagt gactggttca gatgaaagga aaccaccgca
     7141 aaagcgtccg gcggcaggcc gtccacttcc agtgtgaaac gtaatccggt tgacatagca
     7201 acctccggta aaatgaataa tgatccgtac cgtacaactg cggataatgt gataagcggg
     7261 acgacttcag acgttattag ggaaggaaca gaaaatgcta tggttatgtg agaattttgt
     7321 ttaaatatta ctttacagaa acgggtgtct gttcagtcaa aaaaataatc acaagctcaa
     7381 cctcttgttt cttggcgtta ttcaggacag gtacgcatat cacaacga
//
LOCUS       DAZFRJ010000198         7414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000198 DAZFRJ010000000
VERSION     DAZFRJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.200"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..729
                     /gene="yiaT"
                     /locus_tag="ACHKUN_003981"
     CDS             <1..729
                     /gene="yiaT"
                     /locus_tag="ACHKUN_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO6662144.1"
                     /translation="RNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENTKAIP
                     LISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDKRDST
                     AMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGVLYYD
                     ENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYSELPE
                     EITNSPMIDRNESFTFITGVSWRF"
     gene            complement(676..939)
                     /locus_tag="ACHKUN_003982"
                     /pseudo
     CDS             complement(676..939)
                     /locus_tag="ACHKUN_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            943..1044
                     /locus_tag="ACHKUN_003983"
                     /pseudo
     CDS             943..1044
                     /locus_tag="ACHKUN_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1076..1771)
                     /gene="araD"
                     /locus_tag="ACHKUN_003984"
     CDS             complement(1076..1771)
                     /gene="araD"
                     /locus_tag="ACHKUN_003984"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6662145.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1765..2625)
                     /gene="sgbU"
                     /locus_tag="ACHKUN_003985"
     CDS             complement(1765..2625)
                     /gene="sgbU"
                     /locus_tag="ACHKUN_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO6662146.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2618..3280)
                     /gene="ulaD"
                     /locus_tag="ACHKUN_003986"
     CDS             complement(2618..3280)
                     /gene="ulaD"
                     /locus_tag="ACHKUN_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6662147.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3277..4773)
                     /gene="lyxK"
                     /locus_tag="ACHKUN_003987"
     CDS             complement(3277..4773)
                     /gene="lyxK"
                     /locus_tag="ACHKUN_003987"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO6662148.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4777..5763)
                     /gene="yiaO"
                     /locus_tag="ACHKUN_003988"
     CDS             complement(4777..5763)
                     /gene="yiaO"
                     /locus_tag="ACHKUN_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO6662149.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(5776..7053)
                     /gene="yiaN"
                     /locus_tag="ACHKUN_003989"
     CDS             complement(5776..7053)
                     /gene="yiaN"
                     /locus_tag="ACHKUN_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO6662150.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(7056..7412)
                     /locus_tag="ACHKUN_003990"
     CDS             complement(7056..7412)
                     /locus_tag="ACHKUN_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFO6662151.1"
                     /translation="MSRYLFVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALV
                     THSLILFICGALAWGATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLY
                     QLITRSNSLTSPPQGA"
BASE COUNT         1742 a         2000 c         1997 g         1675 t
ORIGIN      
        1 cgcaatattg tggcgttatt tgcgttgcct tttatggcaa gcgcaactgc ttctgaatta
       61 tccattggtg ctggtgcagc ttataatgaa tcgccttatc gcggttataa tgaaaataca
      121 aaggcaattc cgctgattag ttatgaaggt gattcttttt atgttcgtca gaccacatta
      181 ggttttattc tgtcgcaaag tgaaaaaaat gaacttagcc tgaccgcatc ctggatgccg
      241 ctggaatttg accctgccga taatgacgat tatgccatgc aacagcttga taagcgtgat
      301 agtacggcta tggcgggggt tgcctggtat caccacgagc gttggggaac cgtgaaagcc
      361 tctgcggctg cggacgttct ggataacagc aacggctggg tgggggagct atcggtattc
      421 cacaaaatgc agataggtcg cctgtcgctg acacctgcgc tgggcgttct ctattatgac
      481 gagaatttca gtgactatta ctatggcatt tcagagagtg agtcccgtcg tagcggtctg
      541 gcaagttatt ccgcgcagga tgcctgggtg ccctatgtca gcctgacggc aaaatacccg
      601 ataggagagc acgtcgtatt gatggcgagc gcaggataca gcgagctgcc ggaagagatt
      661 accaacagcc cgatgattga tcgtaatgag agttttacct ttatcaccgg cgtgagctgg
      721 cgtttttaat tcaccggtgg atgtcggtgc ggctggtgct ccgtcgtaat gccacaaagg
      781 ttgttgctct ggattgctct gttcataccg ggaacctggc gtctgaatca ggatcttatg
      841 gctgaacaat cccttgcctt caaatgtcgt aaatggcgca tcggcgggca gcaacaggtt
      901 atgcagtgac tcgacgttgt cggcctgctc catgcaatac ggtttaatga cgttggctat
      961 ttccggcaga tttttcgcaa gcacaccggt ttaacgccag cagcgtggaa gcggcgttac
     1021 agcaaggaac atatcaattc gtagtgccgg ggcgatgaag ccccggcgtg agggattact
     1081 gcccgtaata ggcattggcc ccatgcttac gcaggtagtg cttatccagc agttcgtttt
     1141 gcatcgcagg gagctgcggc gcaagctggc gcgagaatag gcccatatag gcgcattctt
     1201 cgagtactac ggcgttatgc acggcatcgg cggcgttttt accccatgcg aacgggccgt
     1261 gagaatgcac cagcaccgcc gggatttgtg ccggactcct gccacgttct tcgaaggttt
     1321 caatgatcac ttcgccggtc tgatattcat attcgccgtt aatctcctct gcggtcatct
     1381 gtcgcgtgca ggggatggca ccatagaaat aatcagcgtg cgtggtgccc caggcgggga
     1441 gatccagccc ggcctgcgac cagatggtgg cgtggcgtga gtgggtatgc acaatgccac
     1501 cgatttcggc gtagcgacgg taaagcgcca gatgcgttgg cgtgtcggac gagggctttt
     1561 tgctaccttc caccaccttg ccgctggcta tctcaaccac caccatatcg tcggcggtca
     1621 tcacgtcata ctcgacgccg gaaggtttga ttaccatcca ttgccgcgtt tcgtctaccg
     1681 cgctgacatt gccccaggtg aacgtcacca gatggtgagc gggaagcgcc agattcgccg
     1741 ccagcacgtc ggctttcagt tgctctaaca tataaatcca gcctcctgca tacgcgcttc
     1801 aatccaacgc cgcgcctgaa taatctccag caccggctct ttggcttttt cggtccacat
     1861 ctcaatcagg aaagaaccgc gatagttcag tttatgcagc gttttgaaga tgccaacgaa
     1921 atcgacgcag ccttcgccaa acggcacgtc gcggaactgt ccgggacttt gctcggtaac
     1981 tggctgggtg tctttcaggt ggatcgcggc gatacggtca atgcccagtt tcagttcggc
     2041 gggaacatca ttgccccagg cgctgaggtt gccgacgtcc gggtagacgg tgaaccacgg
     2101 cgaggcgagc atctcgtccc actttttcca tttactgatg gagttcataa acgcggtgtc
     2161 cataatctcc accgctagca tgacttgtga tgccgccgcc tgttcgaccg cccacgccag
     2221 cccttcagca aaacgttgcc gggtgccttc atcgtggtct tcgtaataga cgtcgtagcc
     2281 cgccagctgg atggtgcgta tgccgagatc gcgtgccaga cgaatcgctt tgctcatgat
     2341 ctcccgtgct cgctggcgta ccgcgtcgtc acggctacca aaaggaaagc gacgatgtgc
     2401 ggacaggcac atcgacggga tcccaacacc cgtttcgatc atcgcggcaa ccagcgaagt
     2461 cctttgtgcg gtgctccagt caagacgtga aagccgttcg tcggtttcat ccaccgacat
     2521 ttcgacaaaa tcaaaaccgc agctttttgc cagcaccagc cgctccggcc aggagagatc
     2581 tttcgccagc gccttttcat aaatccctaa cggatgatta cgcacgcttg cctccccaga
     2641 tagcgtcgat ttgcgcatgg aaatcgccag ccacctgcgc cggatttgct gagcctgcca
     2701 gtgctcgccc ggcaataaac gctttcacgc gaatatcttt aaacagcggc aggtcagcag
     2761 gggtaatccc gccggtaatg gaaagctcaa ggccgatatc ggaaagcgcc ttcatgcgtg
     2821 ccagatcggc ttcgccccac tgttgcccgc tggcctgtgc atcacgaccg cgatgataaa
     2881 tcgcctgccg cacgccaata cgatgccagt cgcgggcgtc gtccagcgtc cagttaccga
     2941 acagctcgat ctgaatttca ccaccgcagc gttgcgccat cgcgtggcct ttttccaccg
     3001 tcgcgagcgg tgcggcgcag atgatggtca tccagttagc gcctgcgcca aatgcctgtt
     3061 gcgcgagcgt ttcaccagcg tcggcgacct tccagtcggc aacgatgatt ttgtcagggc
     3121 actgttcgcg caaggctttc accgcgccaa gcccttcgtt taaacagaga atggtgcccg
     3181 cttcgacaat atcgacgctg tcttttaact gcgtcacgtc gcgctgtgcg gcttcaagtg
     3241 atgagtggtc gagtgccagt tgcagaagtg gtcggctcat aatatgtgct ccttgatgcg
     3301 ggcgtgaaag ccctgaagtg cggcaatgag atgctgatag cgttggtatt tttgttggta
     3361 aagctgatgg acagtcatat ccggcagcag ggtgcgcacc gggtgctgta aatcacgctg
     3421 ggcttcgctg aaatcgcgat aaaccccggt gccgacgcgg gcggcaagtg ctgcaccaaa
     3481 gcagccggtt tcttccacct gcggcagctc aatacgcaga ccgctgacgt ccgccagcat
     3541 ttgcatccag acatcggaat gcgccgggcc gccagtgacg cgcagggtgt gcacatcagt
     3601 aaaacgttcg cgcatccggt tgagatgggt catatggctg aacaccaccc cttcataaat
     3661 ggcctgcaac aggtgtgcgc gggtgtgaat ggcctgcatc ccgtagaaac cgctggtcat
     3721 ctcgagtccg gcgttgctgc cgtacaggaa cggcaggaaa aagagatcgc caccggcctt
     3781 cggcaagctg gcaacggcct ggttgatctc atcaaacgag atttctcccc actgtgcggt
     3841 aaaccattcg aggttgccgg aagaggtggg gctggcttcg tgaacgataa attcaccatc
     3901 gttaacgtag cgaccataga catacggatg cgcttcaccg tcacgtaaac cgcgggttat
     3961 gccgctggtc accgcccagg tgcccatcac cgcattaagg gtaaattcgt cttcgatccc
     4021 ggcgcagagt gcggtggaaa ccacatcaaa caggccgccg acaacgggcg tacccgcttt
     4081 cagaccggtc agtacggctg tctgagcggt gatctccccg cagatttcgg cagatccgac
     4141 aacaggcggc agggtgtgat tgatttcagc aatccccagc cagtcggtga ggcacgggtc
     4201 atattcccca agactcatgt tgtagagatt ggactcggaa atattgctct cttcacagcc
     4261 tttgacgccg gttaaacacc agcgcaggta gtcgtgcgtc atcatcacgc agccaatttg
     4321 cgcgtagcgt tccggttcgt gctctttaag ccaacgtaac agcgataccg gatgcccggt
     4381 ccacaaggtt tgtcgggtca gcgggtagag tttttccggg atgccatctt cctgccagcg
     4441 acgaacgatt tccatcgccc ggcgatccga ggataaaatg gcgttaccga gcggcttgtt
     4501 gtttttatcc agcaaaaaca agccttttcc ttgtgcggag ataccgatac cgacaatttg
     4561 ttccccgcta acgccggaat gggtaagcag ttcgcgaatg acagccatgc agcattgcca
     4621 cagttctgcc atatcgcgct ctgcccaacc tggctgcggg cttaatgcgc acagcggcag
     4681 gcgctgcacg cctgcctccc ggccttcgcg gtcatacagc ccggctttca gccagctacc
     4741 gccacaatct aaccccagcc agtattgcgt catggtttat tgcacctcat ccacggcttt
     4801 cagtagatca gcaccctgcg gaacatcttt tacaaacatg tcgcggacct gagtacccag
     4861 tgcgtcgcta aaggctttgc ggtcgagatc ggtgatgact tccacgcccg cttctttcat
     4921 gccgtcgatg attttttgct gatcttcggc aaccagtttg cgctggtagt tacccgcttc
     4981 ctgcgcagat gaaattagcg cttgctggaa ctccgggctt aagccatcaa acttcgcttt
     5041 gttgatcacc accagaagcg gggaataggc gtggtgcgtc agagaaaggt acttctgcac
     5101 ttcgaaaaat tttgctgacc agacgacgtt gatcgggtgt tcctgagcgt cgatagtgcg
     5161 ggtttccagc ccggtataga cttcggcaaa cggcatcggg atcgggttag cgccaaagac
     5221 tttgaatgcg gcgatattca tcgggctatt gttggtgcga attttcagcc ctttcaggtc
     5281 ggcgggggtt ttaaccggtg cgcgcgagtt ggtgacatcg cgccagccgt tttcccagta
     5341 ggccagtact tttagtcctt taccttcaag tgaggctttc agatcatcac cgactttgcc
     5401 gtcgagcgtt ttatgcgcgt gagcggtatc gcggaacagg aaagggacat caagcaagtt
     5461 catcactggt gataatccgg caaagttatt cgagccggac atttccatat cgatggtgcc
     5521 gccacgtacg ccgctgatca tcgcctgcgc gttaccgagg gtgctatccg gaaacaattt
     5581 taatttcagc tcgcctttgg ttctctcctg caataaatca ttgaattttt tcgccgcaat
     5641 atgttgcgag tcggtttgtg aggtttcata accgaaacgt aaagattgcg ccgccagaga
     5701 agatgtgctg aatgcagcca ggccagcaat gaataatgcg taggttacag agcgtaattt
     5761 cataatattt tccttttaat taatccattt caaagggagg atgatgagat cgggaataaa
     5821 aacaaatacg actaataatg aatataaaac caggacgtaa gggaaaacgc ctctgactgc
     5881 atcatcgaat ttgagttttg ccacccctga aataacgtta agtacattac cgataggcgg
     5941 tgtaattaag ccgattgagc agttaatgat aaacatgaca ccgaaataaa tcggatcgat
     6001 tcctgcctct ttaactaaag gcattaatac cggggtaaga attaataccg tcggcgttaa
     6061 atccatgacc atgccgacaa tcaggatagc caccataatg acgataaaca gcagacgcgg
     6121 tgaatcgacc aacggttgca gtaaatcaga aaccatcatc ggcagttcag caatggtaat
     6181 cagccaggcg gaaacttgtg ccgaggccac cagaaacata accactgagg tggttttcgc
     6241 cgcgccaatg agtacatgcc aaagtgtggc gaaggtcatt tcacggtaaa taactgtggc
     6301 gacaaacagc gcatagaaag cagcaacggc cccggcttcg gtaggggtaa acagaccgga
     6361 gcgaaacccg ccaataatga tgactggaag aaacagcgcc cagataccgg agacaaaaga
     6421 gtgccagatt tcctgcatcg ttgctttttg ctggcggggt aaattaaggc gactggcctg
     6481 ccaccaccag gtaagcatca gcgttgcgcc catcatcatg ccaggggcaa tgcccgccat
     6541 aaacagctta ctgatggata atccgctgga aacgccgaag ataataaacg gaatggaagg
     6601 gggaataatt ggcgcgataa tgccgccaga ggcaatcagc cccgccgccc ggttgaccgg
     6661 gtagttggcg ctgcgcatca tcggcaccag tagagcggca acggcggcgg tatccgcaac
     6721 ggcagaacca gaaaggctcg ccataatcat agctgccagc acgccgacgt agcccagccc
     6781 gcctggttta tgccccacca gtttcatcgg caggtcaaca atgcgttttg acaagccgcc
     6841 cgcattcatg atttcacccg ccagcacaaa gaacggaatc gccagcaggg agaagctatc
     6901 ggctccgttc accagcgttt gtgccatgat ctggacatca aacatgtcca gccagaacat
     6961 taacgccgcc ccgcacaaca acagtgccca ggcaataggc aaaccgatag caataccacc
     7021 caacagacag cccagaaaaa tcagcacagc catgattaag ctccttgcgg tggcgacgtt
     7081 agagaattgc tacgcgtgat gagttgatat aaatgacgca gttcaaagaa tgcgataacg
     7141 aggctggtgg gcagacaagc ggcatacatc aggccgatgg gtaaaccgag gatcggtgaa
     7201 taatcgctcc agtcctgaat tgtttttagc gttgcgcccc aggccagtgc gccacaaata
     7261 aataagatta aggaatgggt aaccagagcg actcgtcgtt gccatgcggg ggagagtttc
     7321 tccaccagaa aggtgacctg aacgtgggcg ttatccataa aagccacaat cgcgccaata
     7381 aatgttaacc agacaaataa ataacgtgac aatt
//
LOCUS       DAZFRJ010000199         7397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000199 DAZFRJ010000000
VERSION     DAZFRJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.201"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..862)
                     /locus_tag="ACHKUN_003991"
                     /pseudo
     CDS             complement(39..862)
                     /locus_tag="ACHKUN_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(916..2634)
                     /gene="proS"
                     /locus_tag="ACHKUN_003992"
     CDS             complement(916..2634)
                     /gene="proS"
                     /locus_tag="ACHKUN_003992"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO6662152.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2746..3453)
                     /gene="tsaA"
                     /locus_tag="ACHKUN_003993"
     CDS             complement(2746..3453)
                     /gene="tsaA"
                     /locus_tag="ACHKUN_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO6662153.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3450..3854)
                     /gene="rcsF"
                     /locus_tag="ACHKUN_003994"
     CDS             complement(3450..3854)
                     /gene="rcsF"
                     /locus_tag="ACHKUN_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO6662154.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3972..4787)
                     /gene="metQ"
                     /locus_tag="ACHKUN_003995"
     CDS             complement(3972..4787)
                     /gene="metQ"
                     /locus_tag="ACHKUN_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO6662155.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4827..5480)
                     /locus_tag="ACHKUN_003996"
     CDS             complement(4827..5480)
                     /locus_tag="ACHKUN_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO6662156.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5473..6504)
                     /gene="metN"
                     /locus_tag="ACHKUN_003997"
     CDS             complement(5473..6504)
                     /gene="metN"
                     /locus_tag="ACHKUN_003997"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO6662157.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6692..7267
                     /gene="gmhB"
                     /locus_tag="ACHKUN_003998"
     CDS             6692..7267
                     /gene="gmhB"
                     /locus_tag="ACHKUN_003998"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO6662158.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1810 a         1922 c         1880 g         1785 t
ORIGIN      
        1 acgccgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag cccaacaaag
       61 cgacccgcct tgatataaat cccaggcttc aggtgcccaa catactgtaa ctgcgtttct
      121 ggcttaaacg cagagacatc accaatacgc atatgcatca gatcggcagg gctaatccag
      181 cccgattgcg ccagtgtaag tggatgcccg gctttggcaa aggcatccgt gacgaattcc
      241 gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc tttcgccagg
      301 ccgctgacgc actttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag
      361 tcaccataaa gggaataaat tcgacaatgc cgcgatagtt ataaccgcta tctttgattt
      421 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
      481 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
      541 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
      601 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
      661 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
      721 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
      781 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga aacaggcggc
      841 agtacgcctt tggtttatcc attttataca atccatgtaa aaaaagggcc ctgaaattca
      901 ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga tgtcaccagt
      961 cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt cgttgtcgag
     1021 gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat cagcaaacat
     1081 cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg cacgcagttc
     1141 gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag
     1201 aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgcctcgtt cgtcgtagtt
     1261 ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac ccatcgtcag
     1321 gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg agtacttggt
     1381 acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg taccctggcc
     1441 atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg gggtagcgac
     1501 atcgcgatcc cagttgatgc cgaagtagtg tttaccatcg atgttagcac cagcagcgaa
     1561 atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt ttaccggacc
     1621 cagtgaaccc ggaccggctt taaccacggc acgaatttct tcttcggtcg cgaaagtcag
     1681 cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg
     1741 caccagcagc gcaaccagcg ggaagctact gccttcaacc gctttaacca gcagagtctt
     1801 aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt
     1861 cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt tcggcgcgat
     1921 agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc
     1981 ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga tagaaccggt
     2041 gtcggcttgt acggcgcgga aatccagccc catgcggctg aagattttgc tgtaggccgc
     2101 atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag agtaagcatc
     2161 tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa
     2221 cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa gctcgttacg
     2281 aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac gctcgccacg
     2341 gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct cttgccacaa
     2401 atcagctggc tgaaccaccg gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc
     2461 acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc aggtatataa
     2521 cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc tgatcacctc
     2581 ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac gcatgttgtt
     2641 acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa aaaagtgatt
     2701 tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag
     2761 cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca
     2821 gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgtg ggtcctgcgc
     2881 cagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg
     2941 cttttcgacc tctgcggtaa aactcaccgc catctctgcc gctggcgcac tttgcgcata
     3001 gctggcactg gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac
     3061 cggcgtacca tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca
     3121 aacaacctct ttcagctcta ccagcgacat accaattggg ttagggcgga aagtagagcg
     3181 tgttgcgaaa acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg
     3241 ccagccgcct tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaacgcttc
     3301 caggccgcga acggcgtcgg cctggttgta gggagcaatg agatgcagtt cgccgttggc
     3361 gctttttacc agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat
     3421 aacgcctatt tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg
     3481 atacatacag cctgacgata gcagcctggc gtaccgctgg tgacttcgca gctatgcagt
     3541 aataccgcat tggccttcat tttcgaggcg ttgatttgca tccgcttacg cgcggttgga
     3601 atgctcggcg gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga
     3661 tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg
     3721 cgcggcgctt tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt
     3781 tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc
     3841 ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag
     3901 cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa
     3961 cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg
     4021 taaacttcat cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta
     4081 tcttcacgcg ttacgatcag gtttacgtac ggggagtctt tatcttcgac aaagataccg
     4141 tctttcgctg gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg
     4201 atttgcgcgt cgtccagaga tcgaggcagc tgcggtgctt ccagctcaac aattttcaga
     4261 ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt
     4321 ttgatcaagc ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt
     4381 ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa
     4441 ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc
     4501 agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc
     4561 aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat
     4621 ttgtctttcg caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg
     4681 ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat
     4741 ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc
     4801 ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg
     4861 cctgcgaact gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc
     4921 gtcgcgttat agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg
     4981 actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc
     5041 accagacccg gcaatgcttc cggtaacagc actttacgga cgatctgcat cggcgtggca
     5101 cccattgcac gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc
     5161 atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caatcgctgc ctgcaaacca
     5221 atcgatgtac cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac
     5281 gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca
     5341 ataatttgcc ccgggcgcgt aacataaagc aggacgccga ccggcagacc aatcacaaag
     5401 ccaaaaaatc cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcaac
     5461 cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca
     5521 ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa
     5581 cttaacgcca ccggcgtaat ccatctgcgc gctaataatg ttgttgttga cgttgaaacg
     5641 acgcgcggtt tcagaaagca gcggggcatc gaccgattga ccggtaaact ccaggcgcaa
     5701 catcggcacg cagtcagtaa atggctccgc ttgcagacgt tcctggtaat cttccgggat
     5761 atccagatgc agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa
     5821 cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc
     5881 acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg
     5941 gcggttaatg tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct
     6001 ggtggcttca tcacacagca atactttggg attgctggct aatgcacggg caatcgccac
     6061 acgttgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc
     6121 aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag
     6181 ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg
     6241 gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcag
     6301 ttcctggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg
     6361 tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg
     6421 cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt
     6481 ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtggca
     6541 tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt
     6601 ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag
     6661 cgtcccatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct
     6721 tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga
     6781 atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt
     6841 ggtagtaacc aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac
     6901 gctgaccgag tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta
     6961 ttattgcccg catcatccgc agggtagtgt tgaagagttt cgccaggtct gcgattgccg
     7021 caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc
     7081 ttcttatatg gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg
     7141 aacaaaagtg ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga
     7201 ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc
     7261 acaatgatta aaagatgagc ggttgaaata aaaatgcatt tttccgcttg tcttcctgag
     7321 ccgactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag
     7381 ccggttcggt tgaagag
//
LOCUS       DAZFRJ010000200         7249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000200 DAZFRJ010000000
VERSION     DAZFRJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.202"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..114)
                     /locus_tag="ACHKUN_003999"
     CDS             complement(<1..114)
                     /locus_tag="ACHKUN_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662159.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHKUN_004000"
     CDS             complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHKUN_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO6662160.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHKUN_004001"
     CDS             complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHKUN_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6662161.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHKUN_004002"
     CDS             complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHKUN_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO6662162.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHKUN_004003"
     CDS             complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHKUN_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO6662163.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1695 a         1957 c         1941 g         1656 t
ORIGIN      
        1 gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga
       61 gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct tcatgtaaaa
      121 ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata gaatatgcct
      181 aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc
      241 atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg ttattcattg
      301 cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt gccagccgat
      361 aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata gcgctctgtt
      421 cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag cgcatcagca
      481 actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt gccacaggcg
      541 cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt ggttccagtg
      601 tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg tcgtctgttt
      661 gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat gccggaaggg
      721 ataacgtcag gagattatcc tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc
      781 ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga
      841 cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca
      901 caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca
      961 caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg
     1021 ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac
     1081 gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa
     1141 tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc
     1201 agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc
     1261 cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg
     1321 ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc
     1381 cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt agctgtcgta
     1441 gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg cggttgaatg
     1501 ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt
     1561 gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg tcagatgatg
     1621 tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc tgcgctttgg
     1681 cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat
     1741 cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcactc atcggtaagc
     1801 gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct ttagcgacca
     1861 gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagtccttcc agccagattg
     1921 ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt cgttgataac
     1981 ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg cactgagcct
     2041 ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag gtggcgatta
     2101 aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc ttaagactgc
     2161 gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt tctaataatt
     2221 ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca gctcgcccgg
     2281 cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat cgcggtcata
     2341 gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc cgctctggac
     2401 acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa aatcgctcac
     2461 gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact ccacggcact
     2521 ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac
     2581 gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac gaatgccggg
     2641 taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca ccatcgatcc
     2701 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
     2761 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
     2821 gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc ggcgacggcg
     2881 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
     2941 aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca tcagatgttt
     3001 ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca gcacgtcgag
     3061 attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcaat
     3121 cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg tgagatttga
     3181 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
     3241 ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
     3301 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
     3361 cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt
     3421 gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc gccgaaacgg
     3481 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
     3541 gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac
     3601 ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag gccagttgat
     3661 ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg
     3721 ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc atcagtgcgc
     3781 catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt cgctccagta
     3841 aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt agatcgcgca
     3901 ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt tcatctggtc
     3961 tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgta
     4021 atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca tatagcactt
     4081 ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat gtgagttgct
     4141 ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg
     4201 tgatactgcc gattccaccc gcgtccagca tattggcttc taatttatgg cgtgcggctt
     4261 cctggagtgt tgcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc
     4321 gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc
     4381 gtacaatccc tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc
     4441 cattggggtt aaagcgattt aaggtaagct ccgccggaag agagattgcg gtacgttcca
     4501 gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga
     4561 atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa
     4621 taacgccgcg atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg
     4681 ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac tgtgccagcg
     4741 ccatcgcatg cagatgagcg gcagccatat ccgcggtcga agcaggcaac ttgtgctggc
     4801 gcaaacgggt aaggatagtt tttagcagtt gttcaccggc ctgctgtaag ccccactgcc
     4861 agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa
     4921 ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc agcgagggca
     4981 attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt
     5041 gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc catgcaatcc
     5101 agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca
     5161 gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt tcatcttcga
     5221 acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agtaaggcct
     5281 ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca
     5341 aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg
     5401 gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact
     5461 ggcaataact gtaaatagct accggaagct ggtgggataa aaacagttcg tctacccggt
     5521 cattaaaatc agccgggcct ttaatcaaca cgtatcgtgg ccgctggctt tcgattaacg
     5581 atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc
     5641 tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct
     5701 tctttatgtg tggcgactcg ttgttcgaag taacggcgca gacgagcgcg atcttcttcg
     5761 ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc acctgcgcga
     5821 ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc ttcagcggtg
     5881 gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg
     5941 cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta
     6001 tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgagc gaaatccata
     6061 atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg
     6121 ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc atagagttga
     6181 ctggcttcac cagtgagttc cggcaccgtc ataactctgt cagaaagcat acctaacaga
     6241 caatcttgca cttccagcgg tgtacgcgtt atctcttcga aacggacgat tttgccgtcg
     6301 cgcattccct ggtaaagcgg ggcagggacc agcgcttccg ttgatgggcc atgattgata
     6361 agcagagcgt agttccagcc gtacttgatt tgatcttcag tggtagatgc gccgccctgg
     6421 atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag gagagatttt
     6481 gcggtgccag gttcccccac caacatcaaa ccgcgtccgg tggcaagagt gactaacata
     6541 cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc gctatcgcca
     6601 aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca aggtgcgtta
     6661 tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg cggacgctgc
     6721 agatgattat tctgcggtga catatattca tccctgaaat gtattaatga aataaacgat
     6781 attttacgcg caataaatgt ttggcgcatt agttgtttga gcggaaaata tctggaaagc
     6841 agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta ctattttcag
     6901 gcctaacacc acagcctgtg caacatcgtg ttccaacgaa cgaattataa ccacggctgg
     6961 accccgttgc aattaagata atgagattta taatcctgac tgttgggacg ttgcgccgga
     7021 tcgaacctat ttcaagtcct ggctaccgga cggggggtgc tgcaggtggt gccctgatcc
     7081 ggagtgcaac atcgtggtcc aacatacgta aaccagtatc gacaggcagt gtaagggtaa
     7141 ggtgctggtg ttgcgctcct taataaggag agcggatgga ctcgctgaca caaaagttaa
     7201 ccgtgctcat tgctgtactg gagctattag tggctctgtt acggttgat
//
LOCUS       DAZFRJ010000201         7234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000201 DAZFRJ010000000
VERSION     DAZFRJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.203"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..3297
                     /gene="evgS"
                     /locus_tag="ACHKUN_004004"
     CDS             <1..3297
                     /gene="evgS"
                     /locus_tag="ACHKUN_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO6662164.1"
                     /translation="LREYADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLII
                     TFPALVTTLHDSMRPLTSPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASV
                     SAGQNDYFIGSNIITSSMISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRF
                     VDALTNEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSM
                     TDENGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDR
                     ENNVSFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQ
                     VDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRG
                     EPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGS
                     SLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEH
                     YFTADYYKNAMLPLENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCIN
                     HWHTLCNLPASDNAVYICGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEI
                     RTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQL
                     QPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSN
                     ALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQ
                     TGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITL
                     PEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMP
                     NMDGFELTRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLS
                     QLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDN
                     RTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQ
                     EIAVFCQKND"
     gene            complement(3353..4498)
                     /gene="yfdE"
                     /locus_tag="ACHKUN_004005"
     CDS             complement(3353..4498)
                     /gene="yfdE"
                     /locus_tag="ACHKUN_004005"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO6662165.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4572..5516)
                     /gene="yfdV"
                     /locus_tag="ACHKUN_004006"
     CDS             complement(4572..5516)
                     /gene="yfdV"
                     /locus_tag="ACHKUN_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO6662166.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5586..>7234)
                     /gene="oxc"
                     /locus_tag="ACHKUN_004007"
     CDS             complement(5586..>7234)
                     /gene="oxc"
                     /locus_tag="ACHKUN_004007"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO6662167.1"
                     /translation="ALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYA
                     AAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDY
                     EELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATME
                     KDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIILGKGAAYSQADEQLREF
                     IESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGKKGW
                     AADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFTTPLVWRDILNI
                     HKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLVNEGANTLDNARNIIDM
                     YKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFGFSGMEIETICRYNLP
                     VTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRH
                     ALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
BASE COUNT         2153 a         1669 c         1484 g         1928 t
ORIGIN      
        1 ctccgggaat acgccgatca tcaaaaagca atggacgcgc ttgcagaagg tgaagtcgat
       61 atagtgttat cacatttagt tacttcgccg cctcttaata gtgacattgc tgcaaccaaa
      121 ccattgataa ttacctttcc ggcgctggta accacccttc acgactcaat gcgaccgctt
      181 acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt accccccaga cgaggtaatt
      241 catcaatcgt ttccaaaagc aacaattatc tcttttacaa atttatatca ggcattagca
      301 tccgtatcag ccggacagaa tgattacttt attggtagta acatcattac cagcagtatg
      361 atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc gccgcgtcaa
      421 tataattttt tcttgaccag aaaagaatcc gtcattctta atgaagtact caatagattt
      481 gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct tgatacagga
      541 aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca gtggattaag
      601 caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc tatgacggat
      661 gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac cttgcaaaca
      721 ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac acagcttaac
      781 cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga aaataatgtt
      841 tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa agcacctgac
      901 agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta tgagctgcat
      961 tcgcaattaa aagagatgta tccggaggtt gaatggatac aggtcgataa tgccagcgct
     1021 gcatttcaca aggttaagga aggtgaactt gatgctctgg tcgcgacaca gttaaattcg
     1081 cgttacatga tcgatcatta ctatcctaat gaactttatc attttcttat tcctggcgtt
     1141 ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa ggatattatt
     1201 aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga aaaatggatt
     1261 aaaatgccca atgtgaccat tgacacatgg gacctatata gcgagcaatt ttatattgtt
     1321 acgacattat ccgttttatt agttggcagt agccttttat ggggattcta cctgttacgc
     1381 tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc attccgaaaa
     1441 gcactctcgg attccttacc gaatccaact tatgttgtaa actggcaagg taatgtcatt
     1501 agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa tgcaatgtta
     1561 ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca tgaagtcaca
     1621 gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga taatggcatc
     1681 gagaaaagat gcattaatca ctggcataca ttatgcaatc ttcctgcaag tgacaatgca
     1741 gtatatattt gtggttggca agatattact gaaacgcgtg atctaattaa tgcactcgag
     1801 gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa gtcagtttct ggcaacgatg
     1861 agtcacgaaa taagaacacc aataagctct attatgggct tcctggaact tctgtcgggt
     1921 tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc caccggacaa
     1981 tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat
     2041 caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg tcactctttc
     2101 ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt tcctgatcat
     2161 tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt actgagcaat
     2221 gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt tcacattgat
     2281 gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt atcgcaggaa
     2341 gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca gcaaacagga
     2401 tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg cgatttgtca
     2461 ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt agaaattagc
     2521 cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc tgaaaagttg
     2581 agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat
     2641 ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca caaagtcagt
     2701 atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag
     2761 ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct tacagccaac
     2821 gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg tttgttcaaa
     2881 ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt
     2941 gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc gaacgatcta
     3001 caactgatgc aggagattct catgactttc cagcatgaaa cgcataaaga tctacccgct
     3061 gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc
     3121 cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca tcagttagaa
     3181 ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa ctctgtaaaa
     3241 gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa tgactaaata
     3301 gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac atttatgatg
     3361 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
     3421 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cccccggctt
     3481 caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc acacttaata
     3541 acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca gctgcctgcg
     3601 tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
     3661 tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag
     3721 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta ttgaaaacat
     3781 caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc cctgtcgcga
     3841 tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
     3901 catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt ccgctgaata
     3961 aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc ggagcatcag
     4021 gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg taggcaggag
     4081 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg aggcgcgggt
     4141 tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct gggcgaaaat
     4201 tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta tcgtgatcat
     4261 tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa tagagtgact
     4321 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc ggctcaactt
     4381 taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca ttaaggacat
     4441 gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta tttgtcattt
     4501 gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg taaaatcaat
     4561 aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc cgtgacgaca
     4621 aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac attaaaccgg
     4681 ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac catcatctgc
     4741 agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag tggcatcaga
     4801 atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt atgtgcagcc
     4861 agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat cagattaaag
     4921 gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt tgccagaaca
     4981 ggtgcccata ctactggctc ctttgccgca gaaattaatg cgctcagatt actattcttc
     5041 ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat agtaattgcg
     5101 ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc accataaata
     5161 ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacccgcaat taatgcacat
     5221 acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccaaacca ggagaaaaag
     5281 aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc aaaaatcatt
     5341 tccctgtttg cccgggtaat agaaacaaat agagccgcag gaagagcgta gtttaatacc
     5401 agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc gctaaaataa
     5461 cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt tagcataata
     5521 gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg agacgatgtc
     5581 agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc actttcagtt
     5641 cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg aataccggtg
     5701 gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc acgaaacgca
     5761 tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggtgatgg tgcaccagcg
     5821 ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat aacgatcgtc
     5881 accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc aaaagcacta
     5941 tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat ggcatagccc
     6001 ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg tttatacata
     6061 tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa ataaatatcc
     6121 tggttctcgc gcaatacgtc gcgcacagca cttaatgcat taaagtaatt taatggttga
     6181 gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg gatatttaaa
     6241 atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc cagcatacct
     6301 tgcatactgg atgcaatatc accaacgact ggcacagcaa tggggcggtt gctgtcaatt
     6361 tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg
     6421 tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc atttgccagg
     6481 gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat ccctttcgcc
     6541 atagacattg gcaggaatgg aatctgagta ctttcaataa actcacgaag ctgttcatca
     6601 gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc ttttgctaaa
     6661 agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga cggattttca
     6721 actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag gacatttgct
     6781 ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat agcgcgtgcc
     6841 aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc atacggtttt
     6901 gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg
     6961 tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt ggccaatgcg
     7021 gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg tttttgggta
     7081 agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa accaatataa
     7141 cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc tacaacacca
     7201 taaatagtgt caatattatt ctgttttaat gctt
//
LOCUS       DAZFRJ010000202         7193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000202 DAZFRJ010000000
VERSION     DAZFRJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.204"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(324..1390)
                     /locus_tag="ACHKUN_004008"
                     /pseudo
     CDS             complement(324..1390)
                     /locus_tag="ACHKUN_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1393..1755)
                     /locus_tag="ACHKUN_004009"
     CDS             complement(1393..1755)
                     /locus_tag="ACHKUN_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6662168.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2292..4205
                     /gene="mtlA"
                     /locus_tag="ACHKUN_004010"
     CDS             2292..4205
                     /gene="mtlA"
                     /locus_tag="ACHKUN_004010"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO6662169.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGEYIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4435..5583
                     /gene="mtlD"
                     /locus_tag="ACHKUN_004011"
     CDS             4435..5583
                     /gene="mtlD"
                     /locus_tag="ACHKUN_004011"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6662170.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5583..6170
                     /gene="mtlR"
                     /locus_tag="ACHKUN_004012"
     CDS             5583..6170
                     /gene="mtlR"
                     /locus_tag="ACHKUN_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO6662171.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6182..6391)
                     /gene="yibT"
                     /locus_tag="ACHKUN_004013"
     CDS             complement(6182..6391)
                     /gene="yibT"
                     /locus_tag="ACHKUN_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO6662172.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6676..7038
                     /locus_tag="ACHKUN_004014"
     CDS             6676..7038
                     /locus_tag="ACHKUN_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO6662173.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1828 a         1800 c         1824 g         1741 t
ORIGIN      
        1 atctatagat taaatatttt ttaaatgtga ctgtcatcct tcagataata aagccatcat
       61 cgcaatagat ggaaaagcac tcctgctctt ttttgattag agccgtcgca ggaggaacaa
      121 ctcatatcat cattgcgatc tcgacgatgt atagtctcat cctgcggcag aacaagacgg
      181 ataaaaaatc caatgagagc acactcatcc ataacttatt aacataatga atattaaaga
      241 aaaatcatcg cagctaatga agtaatatgc aaaaataatt agtccttaca aggagcaaag
      301 ttccataatc tcccccctcc ccctcaatga tccaaataaa gataatgcat ccagctggtc
      361 attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga gtaaaccgcc
      421 acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttagggtc cagttcaatg
      481 gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac cccctgcgcc
      541 tgataagaac cgcctggcac gacaggtaaa atactggtca gtttgccgtg gaacacctgc
      601 ccaggtagcg cgttaaacac cacttctgca tcatcaccag gtttcagacg taacagcgag
      661 ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaaaac catcaccgga
      721 cgcaacggca aggcagctgc gtatgtacct gggcggatca gtacctgagt aatgtagcca
      781 ttgctcggcg cgcgaatgac agtctgctca agattatatt ttgcttcagt aagttgcgct
      841 cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg gctctggatc
      901 tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcgaggaa attttgccgc
      961 gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc tttcagataa
     1021 cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc
     1081 gcttctgtga gctgcgcgcg cagagtcttt atattatgcg tcgccgtcat caggtcagcc
     1141 tgaagcctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag cacctcgccc
     1201 ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt cacctgtggc
     1261 gtgatgggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc
     1321 atcaacaaaa tcaaaccact caccagaaac acgccagcgc cacgtaagtt aaaacaatca
     1381 atagatccat aattagatct ccgggaacgt ggaatagtca gtctttttct cggcggcgag
     1441 ttgatgctcc agccgggaaa ttcggtcaga aagtgcggcg atttccgggt cagtcgcttt
     1501 ctcctgcgac gcaacatgtg actgcatacc ccagccacgc tccggttgat agagcgtcgc
     1561 ccagatccac agaaacggcc agataacatg cagagtaaac aggctcaccc agcccgccgt
     1621 atgaatagcg tcggcatggg gatggttgcg ctttttggca atcagataag ggatgtcatg
     1681 tatcgcgatg attccataaa aaatcaccag aaagacgaag atcagcactc ccaacgcgaa
     1741 atagtttaga aacatatctg cctcggattc acgtttatca gtgttgtttt tgggctggca
     1801 gccagaaggg agtcaggctg atattttgac aataatccgg gttcgcgatt ctcgccataa
     1861 caccaaagaa taatttttag aggtgatgag ttgcttagtt acataacgat tgtatgacga
     1921 aggcataaca tgctgtagat cacatcaggt gaacgccgta agaaaatatc ttgtgattca
     1981 gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga cactactcac atttaaatgc
     2041 catttttagc gaaaatcgcc gccttgttgc ttttttacac aagcgttttg tgatgaacgt
     2101 cacgtcaatt acctctctac cccctatatt tatgtgattg atatcacaca aaaggccgtc
     2161 gactggacag ttaaccgatt cagtgccaga tttcgcagta tctacaaggt ccggctacct
     2221 ctgccgccac attaacaaaa aacctcgggc ttccagcctg cgcgacagca aacataagaa
     2281 ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca aagctttggt cgtttcctca
     2341 gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc accgcgttat
     2401 ttattccaac agggtggtta ccgaacgaga cgctggcgaa gctggtcggg ccgatgatca
     2461 cttatctcct gccgctgctg atcggttata ccggtggtaa gctggtaggc ggcgaacgtg
     2521 gcggcgtagt cggtgccatc accaccatgg gcgttatcgt cggcgcagac atgccgatgt
     2581 tcctcggttc tatgattgca ggtccgctgg gcggctggtg cattaagcac ttcgaccgct
     2641 gggtagacgg taagatcaaa tccggttttg agatgctggt gaataacttc tccgcaggca
     2701 tcatcgggat gatcctcgct attctggcat tcctcggcat tggcccgatt gttgaagccc
     2761 tgtccaaaat gctggctgcg ggcgttaact tcatggttgt ccatgacatg ctgccgctgg
     2821 cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa caatgccatt aaccacggta
     2881 tcttctcgcc gctgggtatt cagcagtccc atgaactggg taaatcaatc ttcttcctga
     2941 ttgaagctaa cccaggtcca ggtatgggcg tgctgctggc gtacatgttc tttggtcgtg
     3001 gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca cttcctgggt ggtatccacg
     3061 aaatctactt cccgtatgtg ctgatgaatc cgcgtctgat cctcgcagtc atcctcggcg
     3121 gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg tctggtttct ccggcatctc
     3181 cgggttctat ccttgctgta ctggcgatga caccaaaagg tgcttatttc gctaacatcg
     3241 cgggtgtgtg tgcggcgatg gctgtctcct tcgttgtctc tactattttg ctgaaaacca
     3301 gcaaagtgaa agaagaagat gatattgaag cagcaactcg tcgtatgcag gacatgaaag
     3361 ctgagtctaa aggcacatct ccgctgtctg cgggcgatgt gactaacgac ctgagccacg
     3421 tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc aagtgcgatg ggcgcaggcg
     3481 tgctgcgtaa gaaaattcag gatgcaggtc tgtcacagat ttcggtcact aacagcgcga
     3541 tcaacaacct gccgccagat gtggacctcg tcatcactca ccgtgacctg accgaacgcg
     3601 ctatgcgcca ggttccgcag gcgcagcata tttcgctgac caacttcctc gacagcggcc
     3661 tgtacaccag cctaaccgaa cgtctcgttg ctgcccagcg ccatactgaa aacgaagtga
     3721 aagtgaaaga cagtctgaaa gacagctttg acgattccag tgctaacctg ttcaaactgg
     3781 gcgcggagaa catcttcctc ggtcgcaaag cagcaaccaa agaagaagcg attcgttttg
     3841 ctggcgagca gctggtgaaa ggcggttacg ttgagccgga atacgttcag gcgatgctgg
     3901 atcgtgaaaa actgaccccg acttatctgg gtgagtatat cgcggtgcca cacggtacgg
     3961 ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt ctgccagtac ccggaaggcg
     4021 tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt gattggtatt gcagcccgta
     4081 acaacgagca cattcaggtt atcaccagcc tgaccaatgc actggatgat gagtccgtca
     4141 tcgagcgtct ggcacacacc accagcgtgg atgaagtgct ggaactgctg gcaggtcgta
     4201 agtaatccaa tcccaccctc tccacatgga gaaggtgggg ttaattgcct gatgcgctac
     4261 gcttatcagg cctacaggat gcatcacaat ttgttgaatt tgcacgttct tgtaggccgg
     4321 ataaggcgct tacgccgcat ccggcgctgc ccctctcctc acggagaggg tttgggtgag
     4381 ggaaaagcct caccccagcc ctctcgggta aaaacattga tgaaggttaa tactatgaaa
     4441 gcattacatt ttggcgcagg taatatcggt cgtggcttta tcggtaaact gctggcagac
     4501 gcgggtatcc aactgacgtt tgccgatgtc aatcaggtgg tacttgatgc cctgaatgcc
     4561 cgtcatagct atcaggttca tgtggttggt gaaaccgagc aggtagatac cgtttccggc
     4621 gtcaatgctg tcagcagcat tggtgatgat gtcgttgatc tgattgctca ggtcgattta
     4681 gtcactaccg ccgttggccc ggttgtgctg gaacgtattg ctccggctat cgccaaaggg
     4741 ctggtgaaac gcaaagaaca aggtaatgaa tccccgctga acatcatcgc ctgtgaaaac
     4801 atggtacgcg gtaccacgca gctgaaaggc catgtgatga acgccctgcc ggaagacgcc
     4861 aaagcgtggg tagaagaaca cgttggcttt gtcgattccg ccgttgaccg catcgtaccg
     4921 ccttcggctt cggcaactaa cgatccgctg gaagtgacgg tagaaacttt cagcgaatgg
     4981 attgtcgata aaacgcagtt caaaggcgca ctgccgaaca tcccaggcat ggagttaacc
     5041 gacaacctga tggcatttgt cgaacgtaaa ctcttcaccc tgaacacggg tcatgctata
     5101 accgcgtacc tcggaaaact ggccggtcat cagaccattc gtgacgctat tctcgacgag
     5161 aaaatccgcg cggtggtaaa aggtgcgatg gaagaaagtg gtgcggtact gatcaagcgc
     5221 tacggctttg acgcagacaa acatgcggcg tacatccaga aaatcctcgg tcgttttgag
     5281 aacccgtatc tgaaagatga tgtagagcgc gtaggccgtc agccgctgcg taaactgagt
     5341 gctggcgacc gtctgatcaa gccactgctc ggtacgctgg aatacagcct tccgcacaag
     5401 aatctgattc aggggattgc tggtgcaatg cacttccgca gtgaagatga tccgcaggct
     5461 caggaactgg cagcactgat cgctgacaaa ggtccgcagg cggcgctggc acagatttcc
     5521 ggtcttgatg ccaacagcga ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa
     5581 taatggtgga ccaggcgcag gacaccctgc gcccgaataa cagattgtca gatatgcagg
     5641 caacaatgga acaaacccag gcctttgaaa accgtgtgct tgagcgtctg aatgctggca
     5701 aaaccgtgcg aagctttctg atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc
     5761 tggtgcttca ggtattccgc aaagacgatt acgcggtgaa gtatgctgta gaaccgttac
     5821 tcgacggcga cggtccgctg ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg
     5881 gcgtcattaa ccgccaggaa tacgaagatg cggagctgct gatggcattg cgtgaagaac
     5941 taaatcacga cggcaacgag tacgccttta ccgacgacga gatccttgga ccctttggtg
     6001 aactgcactg cgtggcggcg ttaccaccgc cgccacagtt tgaaccagca gactccagtt
     6061 tgtatgcaat gcaaattcag cgctatcaac aggctgtgcg atcaacaatg gtcctttcac
     6121 tgactgagct gatttccaga atcagcttaa aaaaagcctt tcaaaagtaa gcaacgtctg
     6181 cttactgccc ctctacctgc ttcggccgat aaagccgacg ataatactcc agacgttgta
     6241 gatataacgg cacgctttca tcgggtattc cggacggaat cgcgttacgg ggaggaagtt
     6301 ttttcagata ctcccggaac gcctggcttg atgccatgaa atctacggct ttatcgataa
     6361 gaagcggaac gttttcacct aatttgccca tgatgatatc tccgtattac cccgcgccgg
     6421 gaatgcgcgg ccgccaattt tagtttaggt tcccggtaaa gatgacatta aggaaaacgt
     6481 gctgaatcct caataatcat ccgctgctgt ttattatctt ttttctatag atttcttaat
     6541 taatcaacga attgcgtagt ttttgagcat gttaatgatt acgttgcaga actattacaa
     6601 tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta ggggctataa atttatcttt
     6661 atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct cagcgatcgc
     6721 ctggacgcca tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc agacaaatat
     6781 gccgagctgg aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct gcgtgaggtt
     6841 catagccaaa aactgagtaa agaagcacag aagctgatga agatgccgtt tcagcgcgca
     6901 attaccaaaa aagagcaggc tgatatgggc aagctgaaga aaagtgttcg cggactggtg
     6961 gttgtgcacc caatgaccgc gctgggccgt gaaatgggcc tgcaggagat gactggattc
     7021 tctaagactg cgttttaagc gcctcagacg actggttcga tcatatcaac ccgcatctgg
     7081 cttgccttag ctggtcaatg cggggactct cctgtctact cgcctaatta ccaaacaatt
     7141 cactcccttt acgtcgattt accgctggtt gatattctga aaatgcattt tta
//
LOCUS       DAZFRJ010000203         7157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000203 DAZFRJ010000000
VERSION     DAZFRJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.205"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(261..1454)
                     /gene="tyrB"
                     /locus_tag="ACHKUN_004015"
     CDS             complement(261..1454)
                     /gene="tyrB"
                     /locus_tag="ACHKUN_004015"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO6662174.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1689..2786)
                     /gene="alr"
                     /locus_tag="ACHKUN_004016"
     CDS             complement(1689..2786)
                     /gene="alr"
                     /locus_tag="ACHKUN_004016"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO6662175.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(2839..4254)
                     /gene="dnaB"
                     /locus_tag="ACHKUN_004017"
     CDS             complement(2839..4254)
                     /gene="dnaB"
                     /locus_tag="ACHKUN_004017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6662176.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4337..5320
                     /locus_tag="ACHKUN_004018"
     CDS             4337..5320
                     /locus_tag="ACHKUN_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO6662177.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5486..5728)
                     /gene="pspG"
                     /locus_tag="ACHKUN_004019"
     CDS             complement(5486..5728)
                     /gene="pspG"
                     /locus_tag="ACHKUN_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO6662178.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5862..6899)
                     /gene="dusA"
                     /locus_tag="ACHKUN_004020"
     CDS             complement(5862..6899)
                     /gene="dusA"
                     /locus_tag="ACHKUN_004020"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO6662179.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1816 a         1894 c         1764 g         1683 t
ORIGIN      
        1 gaaatattct atcgtaacaa acccgatact tattattaaa tttaataatg gttagatgtg
       61 cagaaattat tactccaccc aaaaaaacag gataaggaat tccagataat tgggataagc
      121 ttaacaataa aagaactaac aacgtttatt atctctcacg tagaacgatg gcatcaaaaa
      181 aatcatctat aaagaaagag tgaaagcgac gacagtttaa tttcactgca ggctgggtag
      241 ctccagcctg ctttcctgca ttacatcacc gcagcaaacg cctttgccac acgttgtaca
      301 tttgccgtat ttaacccggc gacacacatg cgaccgctgg cgatgagata gacaccaaat
      361 tcttcacgta gtcggtcaac ctgagcggca cttaaaccgg tataactgaa catgccgcgc
      421 tgattaagca gataatcgaa attgcgttct ggcatctctg tgcttaatac cttcaccagt
      481 tcctgacgca ttgccagaat gcgagtacgc atctcttcta cttccgccag ccagctggct
      541 ttcaatgcct cgtcattcag cactgcagcc accacctgcg caccaaaatt cggcgggctg
      601 gagtagttgc ggcgaactgt tgctttcaat tgccccagta cgcggccagc ggcttcggca
      661 tcttcacaca gaacagaaag tccgccgacg cgctcgccgt aaagggagaa aattttcgag
      721 aacgaattgc tcaccagagc gggtaatcca gcgctggcaa tggcgcgaat agcgtaggca
      781 tcctcttcca taccggcacc aaatccttga taggcaatat cgaggaatgg aataagctcg
      841 cgggatttga gaatttcaat caccgcatcc cactgatcat tagtgagatc ggcacccgtt
      901 gggttgtggc aacatggatg cagcaacaca atactgcggg caggtaatgt tttcagcgtc
      961 gccaacaggt cattaaagcg cacgccgtta gtcgcttcgt cataccaggg gtaagtactc
     1021 acttcgaatc cagccccggc gaatattgct acgtggtttt cccaggtagg atcgctgacc
     1081 cagacgcctg attccgggaa gtagcgtttc aggaaatccg cgcccacttt caatgcccct
     1141 gagccgccaa gggtttgaat ggttgctacg cgctgttgtt gcagtactgg atggtccgca
     1201 ccaaacagca gcggcgcaat ggcatggcga tagctgttaa gcccttccat cggtaaataa
     1261 agcgaagcgc catgaggctg cgcattcagg cgcgcttccg cctccgccac ggcttgcagt
     1321 tgtggaataa ttccgtcttc gttgtagtac agaccgatac ttaaattcac tttgtcgctg
     1381 cgagggtctt ctttaaaacg ctccataagc gtaagaatcg ggtcgccagc gtaggcgtca
     1441 actttttgaa acacgcgatg gttctccagg tttacgggca ggtggttaaa acacaataaa
     1501 ccggaagaag gcgaagatcg agtggatgtt caggagcgaa cggcaattag caacagagtg
     1561 agactcatga caaacgtaca tccgccagag gcacgacctt tataagagca tgaaagaaca
     1621 tcaacttatt gaatttttag gattttattg gccggataag gcattcacgc cgcatccggc
     1681 acagacaatc aaatattaca gaacgataaa tccacgtatt tcatcgcgac ccttgaagtc
     1741 aggcgcgtaa taagttcgta agcgcttact ttcgtcattt cagcgatacg ttctacgggc
     1801 aaaccttcgc cccataaaat gaccggatcc ccggctttgt cctgcgcttg tggacctaag
     1861 tctacgcaga tcatatccat cgccacgcgc ccgacaatcg gtacttcgcg accgttcacc
     1921 agcactggcg taccggacgg cgcggcgcgc ggataaccat cgccatagcc catcgcgact
     1981 acgccaagac gggtatcacg ttcgcttacc caggttccac cataaccaac aggttctccg
     2041 gctttatgct cacgcacggc aatcaggctg gaggttagtg acatcactgg ctgacagcca
     2101 aaatcggcac cggtggagcg atcttccagc ggcgagacgc cataaagaat gatgcccggg
     2161 cgcacccagt caaaatgcga ctgtggccac agcagaatgc cacccgatgc ggcaatggaa
     2221 cgttgaccag gtttgccttc gcaaaaggta ttaaagatag cgagttgttt ctcggttgcg
     2281 ccacattttg gttcatccgc gcgcgcaaaa tggctgacga tattcaccgg ctgacgaacg
     2341 tttttgcact gggtcaggcg atgataaaac gcctcagcct gttccggcct tacgcccaga
     2401 cggtgcatac cggtatcgag tttcatccag acggtaaccg gctcgtccag gctagcctct
     2461 tccagcgcag ccagctgttc ttcgttatgc acggcggtat gaaaatgttg cgcagaaatc
     2521 gtcggcagat ctctggcatc aaaaaagcct tcgagtaaca gtacaggttt ggtgattccc
     2581 cccgcacgca gtcgcagagc ttcttcgaga cgggctacgc caaaggcgtc agcatcgggg
     2641 agcgttcgcg cggtctcaag aagaccgtga ccataagcgt tcgctttcac caccgcaacc
     2701 attttactgg caggggccag ttcacgaaga cgttgcaggt tgtgtcgcag agcgcggcgg
     2761 ttaatcacaa cagttgccgc ttgcatttgt gttccttgat aagtgtttgc tttaattacc
     2821 taattcataa aataattatt attcgtcgtc gtactgcggc cccgcatagt tgtcgaagcg
     2881 cgaccattga ccgttaaagg tcaggcgtac cgtcccgatt gggccgttac gttgtttacc
     2941 gataataatt tccgcgatgc cttttaaatc actgttttcg tgatacacct catcacgata
     3001 gataaacatg atcaagtccg catcctgctc gatagagcca gattcacgca ggtcggagtt
     3061 gaccgggcgt ttgtcggcac gttgttccag agaacggttc aactgggaca gcgccaccac
     3121 cggcacgttc agttctttcg ccagtgcttt cagcgagcgg gagatttctg caatttccag
     3181 cgtacggtta tcggaaagcg ccggtacgcg catcagttgc aggtagtcga tcatgataag
     3241 cccgatgccg ccgtgttcac gggcaatacg gcgtgcgcgg gaacgcactt ccgttggcgt
     3301 caggccggag gagtcatcga tatagatatt gcgtttttcg agcaaaatac ccatggtgcc
     3361 ggaaatgcgc gcccagtctt catcatcgag ctgcccggta cggattttag tctggtcaac
     3421 gcgcgacagc gacgccagag aacgcatcat aatctgttct gaaggcatct ccagcgagaa
     3481 gataagtact ggtttatcct gcaacatcgc cgcgttttcg acgaggttca tcgcaaatgt
     3541 tgttttaccc atcgacggac gcgcggcgac gatgatcaaa tccgacggct gcaagccagc
     3601 ggtttttttg ttgagatcgt cataaccggt gtttactccg gtaacgccat cgtgtggctg
     3661 ctgaaacaac tgctcaatac gcgccacggt tgcgtcgagc acatcggcga tgttcttcgg
     3721 cccttcgtct ttgtttgcac gactttcggc aattttaaag acgcgggatt cagcaaggtc
     3781 cagcagatct tcgctggtac gcccctgcgg atcaaaaccg gcttcagcaa tctcattcgc
     3841 aaccgagatc atctcacgga caacggcacg ttcacgcacg atgtcagcat aagcactgat
     3901 gttcgccgca cttggcgtat tttttgacag ctctgccaga taagcaaaac caccgacgct
     3961 atcgagttgc ccctggcgtt ccagcgattc cgcaagggta atcagatcga taggactacc
     4021 gctttcctgc aaacgcgcca tttcagtaaa gatatggcgg tgtgggcggg tgtaaaaatc
     4081 gtctgccacc acacgctcgg ctacatcgtc ccagcgttcg ttatccagca ttaaaccgcc
     4141 caacaccgac tgctccgctt cgatcgagtg cggaggcact ttcagcccgg caacttgtgg
     4201 atcgcgttcg cggggttcag cctgctgttt gttgaagggt ttatttcctg ccatagtgaa
     4261 tggagttacc gagataaaga atgggtcgaa actttaccat atgaagcagc ccctgacgat
     4321 acgttctgga ggacacatgg caacacgaat tgaatttcac aagcacggtg gcccggaagt
     4381 acttcaagcc gtagagttca ctcctgccga tccggcagag aatgaaatcc aggtcgaaaa
     4441 taaagccatc ggcatcaatt ttatcgacac gtatatccgc agcggccttt acccgccgcc
     4501 atcactaccc agcggattag gtaccgaagc ggcaggcatc gtgagtaaag tcggcagtgg
     4561 tgtaaagcat attaaggcag gcgatcgtgt tgtctatgcg cagtcggcgt taggcgctta
     4621 cagctctgtg cataacatta atgcggataa agcggcgatt ctgcctgcgg caatttcttt
     4681 tgagcaagct gcggcatcct tcctgaaagg cttaacggtt tattatctgc tgcgtaaaac
     4741 ctatgaaatt aaacccgatg aacagttcct gttccacgca gcggctggcg gcgttggctt
     4801 gattgcctgc cagtgggcaa aagccctggg cgcgaaactt atcggcaccg taggaaccgc
     4861 gcaaaaagcg cagagcgcgc taaaagcggg cgcgtggcag gtaattaact atcgtgaaga
     4921 gaatctggtc gagcggttaa aagagatcac cggcggtaag aaagtgcgcg tggtgtacga
     4981 ttccgtgggc agagacacct gggaacggtc gctggattgc ctgcaacgcc gcggcttaat
     5041 ggtcagtttt ggcaactcat caggtgcggt taccggtgtg aacttaggca ttctcaatca
     5101 aaaaggctcg ttgtatgtga cacgcccttc cctgcaaggc tatatcacca cgcgggagga
     5161 attaaccgag gccagtaatg aactgttctc tttgattgcc agcggtgtga ttaaggtcga
     5221 tgtcgccgag cagcagaaat atccgctgaa ggatgcgcag cgtgcgcatg agattctgga
     5281 aagccgggcg acgcaaggtt ccagcctgct gattccataa aagaaatagg gcttccacct
     5341 gggaagccct ttctttttat agttcggctg tatgtagggt acagcacgat gaatctgtta
     5401 gaggcgcaat agtgacagat ttgattatca attcctattt tgttctaagg ataaaacctt
     5461 aggttgtgat catccgcaca atcccttagt aacgccagcg gtcataacgc tgatatttag
     5521 gcacttttgg tgctttaatc gccttaataa cccacaccac cgcaatcgcc agtagtaacc
     5581 acggcagcaa cttaatcatc aatgccagca taccgccgag gaacataatg gccgtcgcca
     5641 caaccagcgc ggcgataatg cccagcaacg aaacgccggt gaccatcagc atgacaaaaa
     5701 agccaatcac aaaaagtagt tccagcatga tgctctccca aatatgaaat ctcttgctgg
     5761 cattacaaga atcatgccaa aaataatcta ttgatttaac agcaaaacgc cccgcgacgg
     5821 tgcgcagggc gtggtgaatt tgactacttt ttggtgaaaa gttaacgctt atccgccacc
     5881 agtttgagcg cgtgttccag cacattaatg tctgcacccg ctttatgggc attttcactt
     5941 aaataacgcc gccactgccg cgcgccagga ataccctgga acaagcccaa catatgccgg
     6001 gtaatatggc cgagatacgt cccctggctg agttcacgct caatgtacgg atacatggcg
     6061 cgcactaccg ccaccggatc ggcatcggta tccgaggaac caaagatctc ccggtctacc
     6121 gccgccagaa tacccggatt ctgatacgcc tcgcgcccga ccatcacgcc atccatatgt
     6181 tgcaggtgtg ctttggcctc ttccagcgac ttgataccac cgttaatcga cattgtcaga
     6241 tgcggaaagt cacgcttcag ttgatacaca cgcggataat cgagcggcgg gatttcacgg
     6301 ttttctttcg gacttaaccc cgaaagccag gctttacgtg cgtggatgat aaacatctca
     6361 cactcgcctt tgccggaaac ggtgttgatg aaatcgcaga gaaattcata gctgtcctgg
     6421 tcatcgatgc caatacgcgt tttcaccgtc actggaatcg acaccacatc gcgcatcgct
     6481 ttcacgcagt cggcaaccag ctgcgtatta cccatcagac acgcaccaaa catgccgttc
     6541 tgcacccggt cagacgggca gccgacattc aggttgatct catcatatcc acgcgcttct
     6601 gccagcttcg cacactgtgc cagcgccgcc ggatcgctac cgccgagttg caacgctacc
     6661 ggatgttctt cttcactgta cgccaggtaa tcacctttac cgtgaataat cgcccctgtg
     6721 gtcaccattt cggtatacag caacgtattg cgggaaagca gacgcaagaa atagcggcaa
     6781 tgtctgtccg tccagtcgag cattggtgca acgctaaacc gaccactcca gtgaacgtca
     6841 gttttttcag gcattgcgct ggtttggttg attttttgca tttcagaatt accgtgcatt
     6901 ttcaaatgta gagattattt tatcgatata tcatggggtt atgttattca gcatcactat
     6961 tcaggaggct caatagcggg gtactatacc ataacaacag gaagcgcctg tctcattgca
     7021 aaagaaaatt gagatcctct caaggcatga agctctcacg aagtgatgga aataatctta
     7081 ttagccgtta actttgttaa ggccaatgat aaacaatcca ggttcgacga taaataaaaa
     7141 atcacacatt aaactct
//
LOCUS       DAZFRJ010000204         7098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000204 DAZFRJ010000000
VERSION     DAZFRJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.206"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..919
                     /locus_tag="ACHKUN_004021"
     CDS             <1..919
                     /locus_tag="ACHKUN_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6662180.1"
                     /translation="LFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAES
                     GDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYDGEQVRV
                     DYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDF
                     LATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRV
                     TFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCAT
                     VRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(975..1517)
                     /locus_tag="ACHKUN_004022"
     CDS             complement(975..1517)
                     /locus_tag="ACHKUN_004022"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6662181.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1501..1812)
                     /locus_tag="ACHKUN_004023"
     CDS             complement(1501..1812)
                     /locus_tag="ACHKUN_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO6662182.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1997..2887)
                     /gene="cyoE"
                     /locus_tag="ACHKUN_004024"
     CDS             complement(1997..2887)
                     /gene="cyoE"
                     /locus_tag="ACHKUN_004024"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO6662183.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2899..3228)
                     /locus_tag="ACHKUN_004025"
     CDS             complement(2899..3228)
                     /locus_tag="ACHKUN_004025"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO6662184.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3228..3842)
                     /locus_tag="ACHKUN_004026"
     CDS             complement(3228..3842)
                     /locus_tag="ACHKUN_004026"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO6662185.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3832..5823)
                     /gene="cyoB"
                     /locus_tag="ACHKUN_004027"
     CDS             complement(3832..5823)
                     /gene="cyoB"
                     /locus_tag="ACHKUN_004027"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO6662186.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5845..6792)
                     /gene="cyoA"
                     /locus_tag="ACHKUN_004028"
     CDS             complement(5845..6792)
                     /gene="cyoA"
                     /locus_tag="ACHKUN_004028"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO6662187.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1935 a         1766 c         1767 g         1630 t
ORIGIN      
        1 attattttct gtaatgctga tagtttcacc aagcgtggtg gccagcgaga aagcgcatga
       61 attgtatgac agcatatacg gtggtaaacc tgctcccgat gttataaata cactgcataa
      121 aatggctgaa tcgggagata tcgacgcgca gagcctgtta ggctgggaat attatcagcc
      181 tcgttatgat accaaacctg atgttcagga agcgattaaa tggttcgagt tagtggctaa
      241 acaaggtgac agagaagctc cgttagcgct ggggggtatc tactacgacg gtgagcaggt
      301 gcgggtggat tatgccaaag cgtatgcact atttaatcag gccgcgcagc acggtgtgaa
      361 tttagcaagg tccaggttgg gcattatgta cgccaatggt cagtatgttg aggtagattg
      421 taagaaagcg aaggaatatc ttgataaagg tgtccacatt tatggtggcc cagaagactt
      481 tctggctact tgtcgaaaag acatgattga cagaaaaacg gttgacgata cgttacccgt
      541 gattacggtt acccgttctg ggatgcgaga taatttttta gataaaggtt tttcctgcat
      601 ggatagcctc ttcgccacca ccaataaatt aggcgaagtg gccaatcttc gcgtgacatt
      661 cagcattcgt cgcccatccg gtaaggagat taaccaaacg gttggcttcg cgcctttcgg
      721 tttaaaccgg ctgaatatta gctttactga ttacctattt ggttcattta ccagtaattc
      781 gtctcttatt ctttataaac cggaatttga gcgaaaatct tgtgcaactg taaggaccac
      841 aatcgttgcc gcaacggcaa ccattaacgg caaggatgtg gagttgctga aagcgggggc
      901 aattgaacaa aagtggtaac gctgctcagc ccccggctga ggcaaaactt gccctggcgg
      961 gtggcgataa taatctaagc cgcgagaata tgctgaatcg ccaaaaacat aggtacagca
     1021 ccaaaagcat ttggtgtcgc tgaaagctga acaaagccga gacgagcata aaaattaatg
     1081 gcggcagggt cagcatcaag ataaacccct ttaattggta atgcctggtg aattattttt
     1141 acatgttcaa aaaaatcaca tagtaggtcc tgaccaaagc cccgcttttg atacttccgt
     1201 gctaccccca acatgactaa tctgacaaca ccaatttctg aaggttgtga accctgaagg
     1261 acgccagaaa cgcgttgttt ttccagcgaa tatgccgtaa aagtacagat gccgatcagt
     1321 tcaccacttt gtctgtcaat aagtgcttta gccgcacagt cgctattacg cacacttttc
     1381 tttagcgatg cgcgtacaaa tttatcgata acagggttac cgcaatcaaa ctgtttttgc
     1441 ccaggataag taatatcaga ctgataatta caggagagga ctatgggcag agtaatctct
     1501 tcatgcttgt cgaccatcgc tgtttttcct tttcattaac gccttgaggg cgagcgtcgg
     1561 ttgagcaggt tcagcgatga gttggttcat cagttcccag ctttgattcg aaagctcacg
     1621 gcgacgttgg ttatcaagca cactttcggc gcgttccata gcagcgctca aaataaaagc
     1681 gcttaaatct accccaacgg cttgcgcagc ttcacgaagt ttgtttttgg cgtcgggact
     1741 agttttgagt tcgacccgcg catttttcag cgttttatcg acatcgggta gagtggctgt
     1801 ttttccaggc attatgacct ctttgattaa ctgaatgaaa tcgtagggag tcgatctcat
     1861 acataatacg gctatcaacc gtatgattaa tttatgttca tctgctcact ctgtcaagac
     1921 atacggttat tagccgtatc tctgatctca gacaataaaa aaggtgccgt agcacctttt
     1981 taatagagag gttttgttac cacacagcag ccagcagcgt atgcgagtcc ggtaccataa
     2041 aatcaacgga catcatcacc gagagggcag tgatggcgat gatagagaag ccgaacagct
     2101 tgcgcgccca gattctgtca tcagcaactt tataaccgcg cagagccata cctaaccacc
     2161 agacgctaac cgccgcggcg accaccagat atttatatcc agcgtaaccg ccaagagaga
     2221 gcatcagcgt ggcaacggca aaggcgatga tatacagcgt gatgtgattc ttcgccaccg
     2281 aaatgccttt taccactggc aataccggaa tgtttgccgc ctggtaatcc ttaaagcgga
     2341 aaatggcgat ggcataggag tgaggcatct gccacaggct gaagatagcc agcaggatcg
     2401 ctgcgccgct atcgaactcg ccggttactg cacagtagcc aatcaccggc ggcgcagcgc
     2461 cggagagcga accaatcaat gtgccgtaga ctgagtggcg tttcatgtac aggctataga
     2521 cgccgacata aaccacaaag cccatcaccc ccagccagca ggccagcgga ttcgcgccaa
     2581 accacagcag cataaagcca gcaataccca gcaaggtggc gtacaccagc gagacagcag
     2641 gagagatcag gcctttaacc agcacccgat tcttcgtcct ttccatcttt ctgtcgatat
     2701 ccctgtcgat gtagttgtta aacacacaac ccgacgccac aaccagtgac accccaacca
     2761 gcgtgtagat aaacagggga taatcaatgc tgccctttga ggccagcagg aatcccccaa
     2821 tcaccgagat caggttgcca aagatgatgc ctggtttcgt tacttgcagg tattgcttaa
     2881 acatcataac cgccgctctt agtgcatcat catgttgtag ttgaggttcc acataatcca
     2941 gatggagcct acaaccagga tagcgatgat tagcacggtg aagacaaacg ccgtcatgtt
     3001 ccagccttca tctgatttgg tattcatgtg caggaagcac accagatgca ccagaacctg
     3061 taccactgcc attgccagga ttgttcccag aattacggcc ggagaggcag cccctgtcat
     3121 caccatccag aacggaatca ccgtcagaat gatcgacagg ataaagcctg tcatgtaggt
     3181 ttttacgctg ccatgggacg cgccgctgtg atcggtagaa tgactcatta catcgccccc
     3241 atcagataaa caacagtgaa cacacagatc caaaccacat ccaggaagtg ccagaacagg
     3301 ctcaggcaca tgatgcgggt acggttagtg ctggtcaggc cgcgacgagc gatttgtacc
     3361 atcagcaccg ccatccagat aagaccggaa gtgacgtgca gaccgtgcgt gccgaccagc
     3421 gcaaagaacg ctgacaggaa gccgctgcga tccggaccca tgccgttaac aatcaggtga
     3481 tggaattcgt agatttccat cccgataaat ccggcaccaa acaaccaggt caacgccagc
     3541 caggagataa cctggctttt gttgtttttg tacatggcga tagccgccat gccgtaggtg
     3601 atggagctga acaacagcaa gaaagtttca accagaacga acggcagttc gaaaatgtcc
     3661 ttacctgtcg ggccgcctgc ggtgccgttc accagaacgg cataggtagc aaacaagatt
     3721 gagaacagaa tgcagtcgct catcaggtag atccaaaatc cgaagatttt ggttccgcct
     3781 gcatcgtggt gcccgtgttc gtgcgcgtgg gcagtcgcgt gcgtcaaagt atcagttgcc
     3841 atttttcagc cctgccttag taatctcatc gaaatgctgg ttttccagtt tttcgatttc
     3901 tgccaccggc acgtagtaat ccacgtcctc gtcgaagctt ttcacgatcc aggtgatgat
     3961 catgcctgcg aagccaacaa tcgccagcca ccagatatgc cagatcatgg cgaaaccgaa
     4021 gatggtggag aaagctgcaa tgacgatacc tgcaccgctg tttttcggca tatgaatttc
     4081 ttcatagtgg tcaggctttt tatacgcttc gcctttctct ttcatttccc agaatgcatc
     4141 acgctcgtga acgtgcggca caacggcaaa gttatagaac ggaggcgggg aagaggttgc
     4201 ccactccagc gtacggccac cccacgggtc gccagtcagg tcacggttct ggtcgcggtc
     4261 gcgaatagaa acgtacatct gaataacgag gcagagaata cccagcgcaa tcagtactgc
     4321 accgctagct gcaatcatca gcatggtgtg gaactgcggg tcaatctgct ggctcaaacg
     4381 acgggtcatg cccatgaagc ccagcgcata cagcggcata aaggcaacga agaagccgat
     4441 gatccagaac cagaacgcgc gtttacccca ggtttcattc agtttgaaac cgaaggcttt
     4501 aggccaccag taggtcatcc ctgcgaagca gccgaagacc acgccgccga tgatcacgtt
     4561 atggaagtgc gcaatcagga acaggctgtt atgcagaacg aagtccgcgc ccggtactgc
     4621 cagcagcacg ccagtcatcc cgcccaccga gaaagtgacg ataaaaccga tggtccacag
     4681 catcgcagaa tggaacacga tgcggccctg atacatggtg aacagccagt tgaagatctt
     4741 caccccggtc gggatggcga taatcattgt ggtgatacca aagaaggcgt ttacgttcgc
     4801 gcccgcaccc atcgtaaaga agtggtgcag ccaaacgatg aacgacagca cggtgataca
     4861 gacggttgcc cataccagcg aggtataacc aaacagacgt ttacgcgaga aggttgccgc
     4921 aatttcggag aacacaccga aaacaggcag gatcaggatg taaacttccg ggtggcccca
     4981 ggcccaaatc aggttgatgt acatcatcat gttgccaccc atatcgttgg taaagaaatg
     5041 ggtgcccaga tagcgatcca gggtcaacaa cgcgacggta accgtcagaa ttgggaagga
     5101 agcaataatc agtacgttcg cgcacagtga tgcccaggta aatactggca tcttgaacat
     5161 ggtcatgccc ggtgcgcgca tcttcagaat ggtaacgaag aagttgatac cggtaagcgt
     5221 cgtacctata ccggatagct ggagactcca tatccagtaa tcgacaccga ctcccggact
     5281 gtactctatt cccgatagcg gtggataggc cagccagccg gtctgcgcaa attcgcccac
     5341 gccgagagaa acgttaacca gaatcacacc aacaacagta aaccagaagc ttaagttgtt
     5401 gaggaacggg aacgcaacgt cacgcgcgcc gatctgcagc ggaaccacca ggttcatcag
     5461 accgataacg aaaggcatcg ccacgaagaa gatcataatc acgccgtgcg cggtgaagat
     5521 ctgatcgtag tggtgaggtg gcaggaagcc cgcttcgccc gccgaggcaa gagcctgctg
     5581 gctacgcatc ataatggcgt cagcaaaacc acgcagcaac atcacaatcg ccacgatgat
     5641 atacatgata ccgaggcgtt tatggtcgac ggaggtcagc cactctttcc acaggtaggt
     5701 ccacttaccg aagtaagtga tcaggccaac gagcgccaga cctcccaaaa taatgccagc
     5761 gatcgtaacc atgacgatag gttcatggaa cgggactgca tcaagtgata attttccgaa
     5821 catctttatt cttcctcaac ccctttaatg ggcggattcc gcgtggctca tgtccatgcc
     5881 ttccatacct tcgtgtgcac tgtgctcacc ttctggctgg gtcatgtcca tgctcttacc
     5941 gtgagccata aacttgttaa tcacatcggc aaacaagtct ggtttcacgt tggagaaata
     6001 ttccacctgg ttgtattcgc taggcgcggc cagtttttcg aacgcagcca tgtcagacat
     6061 ggtgttcggc gactgtttcg cttttgcgac ccattggtcg aattcggcgc gatccggtgt
     6121 tgcaatagct ttgaacttca tgcctgagaa gcccggcccg ctgtagctgg cggagatacc
     6181 gtcataagta ccgggttcgt tggcgatcag atgcaggcga gtctgcatac cggccatggc
     6241 ataaatctgg ctacccagac gcggaatgaa gaaggagttc atcacggagt tggaggtcac
     6301 tttgaagtac accggagtgt tcgccgggaa agcgatttca ttcacggtag caatgccctg
     6361 ttccgggtag atgaagaacc atttccagtc catggaaacc acttcgatgg taatgggctt
     6421 ctcgtcgtgt gccagcggct tgctaggctc aagagcgtga gtggttttcc aggtcagtac
     6481 cgcaaggaag atgatgatta agataggtac cgtccagacc acagcttcca ctttattgga
     6541 gtgtgaccag ttcgggctgt acttagcatc tttattgctc gcacgatact tccaggcgaa
     6601 accaacagcc atcaagattg cgggaataac gacaatcaac atcaggccaa atgccgtcag
     6661 tatcagtgaa cgttgctcca gaccaatctg tcctttggga tctaacagcg cagaattaca
     6721 gccactgagc aatacagtgc ctgcaaataa tgacaaccat cccaaacttt tattgtattt
     6781 cctgagtctc atttaacgac ctcaattcca cgggatctgg tggcgtttaa agtgtgtggg
     6841 cattttacgg gaaggttaca ttactgtaaa catgattaaa ttcctgttac ttggatttgg
     6901 ctgtcaggtc acatatgttg caaaacatat caagttgatt ttaagaagct gttgcaaaag
     6961 ggcgttataa cgaaagggga gtgcagaagg agtttggacg ggcaattggt ataaccaatc
     7021 tggaataaaa caattgttta acaaataatt atcattatgg tgacaattgg gtgggggaaa
     7081 ataaagactc attagctg
//
LOCUS       DAZFRJ010000205         7097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000205 DAZFRJ010000000
VERSION     DAZFRJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.207"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..74
                     /gene="yobI"
                     /locus_tag="ACHKUN_004029"
     CDS             9..74
                     /gene="yobI"
                     /locus_tag="ACHKUN_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO6662188.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            213..824
                     /gene="ruvA"
                     /locus_tag="ACHKUN_004030"
     CDS             213..824
                     /gene="ruvA"
                     /locus_tag="ACHKUN_004030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO6662189.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            833..1843
                     /gene="ruvB"
                     /locus_tag="ACHKUN_004031"
     CDS             833..1843
                     /gene="ruvB"
                     /locus_tag="ACHKUN_004031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO6662190.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1990..2775)
                     /gene="znuB"
                     /locus_tag="ACHKUN_004032"
     CDS             complement(1990..2775)
                     /gene="znuB"
                     /locus_tag="ACHKUN_004032"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO6662191.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2772..3527)
                     /gene="znuC"
                     /locus_tag="ACHKUN_004033"
     CDS             complement(2772..3527)
                     /gene="znuC"
                     /locus_tag="ACHKUN_004033"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO6662192.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3606..4538
                     /gene="znuA"
                     /locus_tag="ACHKUN_004034"
     CDS             3606..4538
                     /gene="znuA"
                     /locus_tag="ACHKUN_004034"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO6662193.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4554..5876
                     /gene="mepM"
                     /locus_tag="ACHKUN_004035"
     CDS             4554..5876
                     /gene="mepM"
                     /locus_tag="ACHKUN_004035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO6662194.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPLDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5996..6967
                     /gene="lpxM"
                     /locus_tag="ACHKUN_004036"
                     /gene_synonym="msbB"
     CDS             5996..6967
                     /gene="lpxM"
                     /locus_tag="ACHKUN_004036"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6662195.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         1850 a         1735 c         1839 g         1673 t
ORIGIN      
        1 gagtcgcaat gtatattttt attacgcatt tcttcactga atatgtaata ttcaaatatt
       61 tgcttccaat ataacctgta gaataaatta tactgcgcca tttttcagat cgttgggacg
      121 cctcgcaagt tttcttcatc cttcgctgga tatctatcca gcattttttt atcatacagc
      181 attatctttg attcattacg caggagcgtc atgtgatagg cagactcaga ggcatcatca
      241 ttgaaaaaca acccccgctg gtgttaattg aagtgggcgg cgtaggctat gaagtgcata
      301 tgccgatgac ctgtttttat gaactccctg aagcgggtca ggaagcgatc gttttcaccc
      361 actttgtggt gcgtgaagac gcgcaactgc tgtacggttt taacaataaa caagagcgca
      421 cattgttcaa agagttgatc aaaaccaacg gcgtcggccc gaagttggcg ctggcgatcc
      481 tctccggaat gtcagcgcag cagttcgtta atgccgttga gcgtgaagaa gtgggggcac
      541 tggtgaaact gccgggtatt ggcaaaaaaa ccgccgaacg cttgattgtt gaaatgaaag
      601 accgatttaa aggtttgcat ggcgatctct ttacgccagc cgccgacctg gtactcacgt
      661 cacctgccag cccgacgacc gacgatgctg aacaggaagc ggttgccgcg ctggtggcgc
      721 tgggctataa accacaagaa gcaagccgca tggtgagcaa aatcgctcgc cctgacgcca
      781 gcagtgaaac attaattcgc gaagccctac gcgccgcgtt atgaggtaaa ggatgattga
      841 agcagaccgt ctgatttctg ccggtaccac tttgccggaa gatgtggcag atcgcgccat
      901 tcgccctaaa ttactggaag agtatgttgg tcagccgcag gttcgttcac agatggagat
      961 tttcatcaaa gcagcgaaac tgcgcggcga tgccctcgat catctgttga ttttcggtcc
     1021 tccggggttg ggtaaaacta cgcttgccaa cattgtcgcc aatgaaatgg gcgttaattt
     1081 acgcacgact tctggtccgg tgctggaaaa ggcgggcgat ctggcagcga tgctcactaa
     1141 ccttgaaccg catgacgtgt tgtttattga tgagatccac cgtctttcgc cagtggtgga
     1201 agaggtgttg tatccggcaa tggaagacta ccaactggat atcatgattg gtgaaggtcc
     1261 ggcggcacgc tccattaaaa ttgatttgcc gccgtttacc ctgattggtg caaccacgcg
     1321 cgcaggttcg ctgacatcac cgttgcgcga tcgttttggt attgtgcagc gtctggagtt
     1381 ttatcaggtg ccggatctgc aatatatcgt cagtcgcagc gcacgcttta tggggcttga
     1441 gatgagtgat gacggcgcgc tggaagttgc tcgtcgagct cgcggtacgc cgcgcattgc
     1501 caaccgtctg ctgcgtcgag tgcgtgattt cgccgaagtg aagcacgatg gcaccatctc
     1561 ggcagatatc gctgctcagg cgttggatat gttgaatgtc gatgctgaag gtttcgatta
     1621 tatggaccgc aagttattgc tggcggtaat cgataagttc tttggtgggc cggtaggtct
     1681 ggataacctt gcggcagcca ttggcgaaga acgtgaaacc attgaggatg tgctggaacc
     1741 ttatttgatt cagcaaggct ttttgcagcg tacaccgcgt gggcgtatgg cgacgacgcg
     1801 ggcgtggaat cactttggca taacgccgcc agaaatgccg taagtcggat tggtcgctcg
     1861 cgccgcatcc gacactggca gtgtgtctga tgcgacactg ttcgcgtctt atcaggccag
     1921 ccagagatag cgaaccgtag gtcggataag gcgctcgcgc cgcatccgac aaatgtgttc
     1981 agcgatagat tagctggcct gctttttcat catactgaga ataaataaca gtgccgcaca
     2041 tagcaccacc gacggacccg ccggcgtatc gtaaaacgcg gaaaaggtta aaccgccagt
     2101 tactgccacc atccccacca aaacagcgac accagccatc tgttccggcg tacgggcaaa
     2161 gcgacgcgca gtagctgcag gaataatcag cagtgaagta ataatcaacg cgccaacaaa
     2221 tttcatcgct acaccaatcg tcaatgccgt caccagcatc aacaacaatt tcacgcgctg
     2281 taatttcaca ccatcaacaa acgccagatc cgggctaatc gtcatcgaca gcaaattgcg
     2341 ccattgccag aacaaaatag ccaccacgat gaccacgcca atcgcaatag cgatgagatc
     2401 ttctggcgtc actgccagca aatcaccgaa caggtaagcc atcaaatcaa cacgaatgtt
     2461 agacatcaga ctaacgacca ccaggcccag cgacagggca ctgtgcgcca taatccctaa
     2521 taacgtgtca atcgccagct gtggacgctt ctccagccat accagaccgc ccgccaggag
     2581 cagcgtaacg gcaattaccg catagaatgg attcacgtcc agcaacaaac caaacgcgac
     2641 gccaagtaat gaggcatgag ccagcgtatc accgaaataa gacatacgac gccagactac
     2701 aaacgaaccc agcggacccg cggcacaggc gagcatgatc ccggctaacc aaccgggaaa
     2761 taataattca atcatgagcg atcatttccc cgacgcaaaa caattcgtcc ctgtaaatcg
     2821 tgacgatgat tatgatgatg gcgatagata cccagttgtt cagcaccacg aggaccaaac
     2881 atagaaataa actccggatg tagggaaaca acttccggtg tgccggaaca acaaatgtgg
     2941 tgattcagac acagcacttc atcggttttt gccattacca gatgcagatc gtgagagacc
     3001 attaaaacgc cacaatccag ttcgcgacgc aactggtcaa taaggtcata taacgccacc
     3061 tggccattta catccacgcc ttgagtgggt tcatccagca ctaataattg cggtcgattt
     3121 aacaatgctc gcgctaacag tacacgctgt gtttcgccac cagagagctt ttgcatcggt
     3181 gcgttaatca gatgcccggc ctggacacgt ttcagtgcag gcaaaatatc ttctttatgt
     3241 gtgccagggc gtaagcgtaa aaaacggttt acggtcagtg gcaacgtggt gtcgagatac
     3301 agcttctgcg gtacatagcc gatgcgtagt tttccgttgc gcttgataac cccttcatcg
     3361 ggtgttacca gcccgagcac tacccgtacc agtgtcgact tacctgcgcc atttggccca
     3421 agtaaagtca aaatttttcc aggtttaagt tccagcgaca catcagagag gacgcggcgt
     3481 tggccaaaag aaaccgagac attttccagg gaaaccagac ttgtcatgtt aattttagtc
     3541 ttgcagtagt catgaaatgt tataatatca cacttctcat attcattacg atgattggtc
     3601 gcattatgtt acataaaaaa acgcttcttt tcgcagcatt atccgccgct ctctggggag
     3661 gtgcaacaca ggccgcagat gccgccgttg tcgcttcgct taaacccgtt gggttcatcg
     3721 cttctgccat tgctgatggt gtaacagaaa cggaggtttt actgcctgac ggcgcttcag
     3781 aacatgatta ttcactgcgt ccatcggatg taaaacgctt acagaacgcg gacttagtcg
     3841 tttgggttgg cccggagatg gaagcgttta tgcaaaaacc ggtaagcaaa ttaccaggag
     3901 cgaagcaggt aacgattgcg cagcttgaag atgtgaaacc gctactgatg aaaagtattc
     3961 acggcgatga tgatgatcac gaccacgcgg aaaaaagtga cgaagatcac catcacggcg
     4021 atttcaacat gcatctttgg ctttccccag agatagcgcg ggctacagcg gttgcaatcc
     4081 atggaaaatt agtggaactt atgccgcaaa gtcgagccaa acttgacgcc aacctgaagg
     4141 attttgaggc acaattagcc tcaaccgaaa cgcaggttgg taacgagctc gcgccgctca
     4201 aggggaaagg ttatttcgtt tttcacgatg cttacggcta tttcgaaaaa cagtttggac
     4261 tgacaccgct tggtcatttt accgttaacc ctgagattca acctggcgcg cagcgtttac
     4321 atgaaataag aacacagttg gttgagcaaa aagcaacctg cgtttttgct gagccacagt
     4381 tcaggccagc ggtcgttgag agcgtcgcac gagggacatc cgttcgtatg ggaacgttgg
     4441 atcccctggg gacgaatatc aaactgggta aaacaagcta ttcagaattc ctgagtcaat
     4501 tagccaacca gtatgcgagc tgcctgaaag gagattaatg aggaagtgat tacgtgcaac
     4561 agatagcccg ctctgtcgcc ctggcgttta ataatttacc gcgaccacac cgcgttatgt
     4621 tggggtcgct caccgttctt actctggccg tcgctgtctg gcggccttat gtttatcacc
     4681 gtgatgccac gccaattgtc aaaaccattg agctggaaca gaacgaaatt cgttcgctct
     4741 tacctgaagc cagtgagccg attgatcaag ctacacaaga agatgaagcc attcccctgg
     4801 acgaactgga tgacaaaatc gccggtgaag cgggcgtgca tgaatatgtt gtttccactg
     4861 gcgatacgct aagcagcatt ctcaatcagt atggtattga tatgggtgat atcacccaac
     4921 tggctgcggc cgacaaagaa ttgcgtaacc tgaaaatcgg tcaacaactc tcctggacat
     4981 taaccgcgga cggcgaactg cagcgcctca cctgggaagt gtctcgtcgt gaaacccgaa
     5041 cctatgaccg tactgccgct aacggtttta aaatgaccag cgaaatgcag caaggagagt
     5101 gggttaacaa tctgctgaaa ggtaccgtcg gggggagctt tgttgccagc gccagaaacg
     5161 ccggtttaac cagcgccgaa gtgagcgcag tgattaaagc catgcagtgg caaatggatt
     5221 tccgcaaact gaaaaaaggc gatgaatttg cggtgttaat gtctcgagaa atgcttgatg
     5281 gtaaacgtga gcaaagccag ctgctgggcg tacgtttgcg ttcagaaggt aaagattatt
     5341 acgctatccg cgctgaagat ggcaaattct acgatcgtaa cggtactggt ctggcgaaag
     5401 gattcttgcg attcccgacg gcgaaacagt tccgtatctc gtctaacttt aacccgcgtc
     5461 gaactaatcc ggtgacaggt cgcgttgcac cacacagagg tgttgatttc gccatgccac
     5521 aaggtacgcc agtgctttca gtgggtgacg gtgaagtggt ggttgccaaa cgcagtggcg
     5581 cagcaggtta ttatgtggct attcgtcatg gtcgcagcta caccacgcgt tatatgcact
     5641 tgcgcaagat tctggtgaaa ccgggacaga aggtgaaacg tggcgaccgt atcgcgcttt
     5701 ccggtaatac cggacgttca actgggccgc atctgcacta tgaagtatgg ataaaccagc
     5761 aggccgtaaa cccgctgacg gcaaaactgc cgcgtactga agggctgacc ggctccgatc
     5821 gtcgcgaatt cctggcgcag gccaaagaga ttgtgccgca gctacggttt gattaattaa
     5881 catccattcg cagccggtac gcagtcagta ccggcttttt ttatttggtg cggggcaagt
     5941 tgcgccgcta cactatcacc agattgattt ttgccttatc cgaaactgga aaagcatgga
     6001 aacgaaaaaa aataatagcg aatacattcc tgagtttgat aaatcctttc gccacccgcg
     6061 ctactgggga gcatggctgg gtgtagcagc gatggcgggt atcgctttaa cgccgccaaa
     6121 gttccgtgat cccattctgg cacggctggg acgttttgcc ggacgactgg gaaaaagctc
     6181 acgccgtcgt gcgttaatca atctgtcgct ctgctttcca gaacgtagtg aagctgaacg
     6241 cgaagcgatt gttgatgaga tgtttgccac cgcgccgcaa gcgatggcaa tgatggctga
     6301 gttggcaata cgcggaccgg agaaaattca gccgcgcgtt gactggcaag ggctggagat
     6361 catcgaagag atgcggcgta ataacgagaa agttatcttt ctggtgccgc acggttgggc
     6421 cgtcgatatt cctgccatgc tgatggcctc gcaagggcag aaaatggcag cgatgttcca
     6481 taatcagggc aacccggttt ttgattatgt ctggaacacg gtgcgtcgtc gctttggcgg
     6541 tcgtctgcat gcgagaaatg acggtattaa accattcatc cagtcggtac gtcaggggta
     6601 ctggggatat tatttacccg atcaggatca tggcccagag cacagcgaat ttgtggattt
     6661 ctttgccacc tataaagcga cgttgcccgc gattggtcgt ttgatgaaag tgtgccgtgc
     6721 gcgcgttgta ccgctgtttc cgatttatga tggcaagacg catcgtctga cgattcaggt
     6781 gcgcccaccg atggatgatc tgttagaggc ggatgatcat acgattgcgc ggcggatgaa
     6841 tgaagaagtc gagatttttg ttggtccgcg accagaacaa tacacctgga tactaaaatt
     6901 gctgaaaact cgcaaaccgg gcgaaatcca gccgtataag cgcaaagatc tttatcccat
     6961 caaataaaaa acagcctctc aaattattga gaggctgtac ttgataaaaa atctcaatga
     7021 gtgttctcac cattttgagt ttttagatta gtacgctatt gagttaatcg tagcgtgata
     7081 atttcaaaag gtttgaa
//
LOCUS       DAZFRJ010000206         7087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000206 DAZFRJ010000000
VERSION     DAZFRJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.208"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..819
                     /gene="gadX"
                     /locus_tag="ACHKUN_004037"
     CDS             <1..819
                     /gene="gadX"
                     /locus_tag="ACHKUN_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO6662196.1"
                     /translation="SLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDK
                     CVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNK
                     NLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLA
                     RIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHS
                     VSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1187..1915
                     /gene="gadW"
                     /locus_tag="ACHKUN_004038"
     CDS             1187..1915
                     /gene="gadW"
                     /locus_tag="ACHKUN_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO6662197.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2278..5391)
                     /gene="mdtF"
                     /locus_tag="ACHKUN_004039"
     CDS             complement(2278..5391)
                     /gene="mdtF"
                     /locus_tag="ACHKUN_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO6662198.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5416..6525)
                     /gene="mdtE"
                     /locus_tag="ACHKUN_004040"
     CDS             complement(5416..6525)
                     /gene="mdtE"
                     /locus_tag="ACHKUN_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO6662199.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6633..6911
                     /locus_tag="ACHKUN_004041"
     CDS             6633..6911
                     /locus_tag="ACHKUN_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662200.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6912..>7087)
                     /gene="gadE"
                     /locus_tag="ACHKUN_004042"
     CDS             complement(6912..>7087)
                     /gene="gadE"
                     /locus_tag="ACHKUN_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO6662201.1"
                     /translation="NQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRR
                     MSDIVTLGITSYF"
BASE COUNT         1794 a         1697 c         1745 g         1851 t
ORIGIN      
        1 tcattacatg ggaattgtct aattgcgtac gcaagacata aatatattct caccatggtt
       61 aatggtgaat atcgctattt taatggcggt gacctggttt ttgcggatgc aagccaaatt
      121 cgagtagata agtgtgttga aaattttgta ttagtatcaa gggatacgct ttcattattt
      181 ctgccgatgc tcaaggagga ggcattaaat cttcatgcac ataaaaaaat ttcttcatta
      241 ctcgttcatc actgtagcag agatattcct gtttttcagg aagttgcgca actatcgcag
      301 aataagaatc ttcgctatgc agaaatgcta cgtaaaagag cattaatctt tgcgttgtta
      361 tctgtttttc ttgaggatga gcactttata ccgctgcttt tgaacgtttt gcaaccgaac
      421 atgcgaacac gagtttgtac ggttatcaat aataatatcg cccatgagtg gacactagcc
      481 cgaatcgcca gcgagctgtt gatgagtcca agcctgttaa agaaaaaatt gcgcgaagaa
      541 gagacatcat attcacagtt gcttactgag tgtagaatgc aacgtgcttt gcaacttatt
      601 gttatacatg gcttttcaat taagcgagtc gcagtgtcct gtggatatca cagcgtgtcg
      661 tatttcattt acgtctttcg aaattattat gggatgacgc ccacagagta tcaggagcga
      721 tcggcgcagg gattgccgaa ccgtgactcg gcggcaagta ttgttgcgca aggaaatttt
      781 tacggcactg accgttctgc ggaaggaata agattataga attttactca gacataaaaa
      841 aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta ataaaccgtt
      901 ataacactcc ctgttggcac gggaaacttt gtgctctcag taagttaaat ataactttta
      961 ctggaaataa gatcagccat ttttttataa acataagcta tacgctgtgc gaaaatatat
     1021 tcgtgctgca tttacttatt atcaattaac tgttatgcaa aactactttg tggataaatt
     1081 ttggtcctac caaatctggc agtttttgcg ctaagaaaca gtctggcaac atttcattag
     1141 tatactgaaa ttgaaataat cgcagtatga aatataaggg ataatcatga ctcatgtctg
     1201 ctcggtgatc ctcattcgtc gttcattcga tatttatcat gaacagcata aaatatcgct
     1261 gcataacgag agtatcgtgc tgctggagaa aaatttggca gacgattttg cgttttgttc
     1321 accggatacg cgacgactgg atatcgatga gctgacagtt tgccattact tacaaaatat
     1381 tcgtcagcta ccacgcaatt tagggttaca tagcaaagac cgtctattaa ttaaccagtc
     1441 accccccatg ccgctggtga cggcgatttt tgatagcttc aatgaatccg gggtaaattc
     1501 accgatactg agcaatatgc tctacctttc ctgtttatcg atgttttctc ataagaaaga
     1561 actgatcccc ttacttttca atagcatcag cactgtttca ggaaaagttg aacgccttat
     1621 tagctttgat atcgccaaac gttggtatct gcgcgatatc gcggaaagaa tgtataccag
     1681 cgagagtctc atcaaaaaaa agttgcagga tgaaaatacc tgtttcagta aaatattact
     1741 cgcttccagg atgtcgatgg ccagacgatt actcgagtta cgtcaaattc ctctgcatac
     1801 tattgcagaa aaatgtggct atagcagtac gtcgtacttt ataaacacat ttcgacaata
     1861 ttatggtgta acgccacatc agtttgcgca acattcgcca ggtacctttt cctgacatat
     1921 tttgcatttg aatattggtc aggatctcac acctgcttca tgtgaaactc ttccctgatg
     1981 atttctgccg ggctaccggc tagttctctt tcgcagtttg ccaggcggta taggtatttt
     2041 caggaggaaa agactcggca tgtttgggat tattaagctg acaattcata cgattacggg
     2101 aatgtgggtc agtattgtgc tgttcaaatt gatgacgaat ggctggagtg gtttctattt
     2161 tcagtgctgt gtactaagcc tggtattcct gacggttagc tggttgttga gtggtgaatg
     2221 gttagcagga aagagtaagg ctgaaccttc acgttcaacc ttactctcat ttacacgtta
     2281 cgctttttta aagcgggcaa agagatgttc cactacaacg aaaaagaccg gaacgaagta
     2341 aattgccagc actgttgcgg caaacatccc gcccatcacg ccggtaccta ccgcgttttg
     2401 cgcgccagaa ccggcaccat gactgataac cagcggcagc acgccgagaa taaaggccag
     2461 agaggtcatc aggattgggc gtaaacgcat ccgcgccgct tcgatgattg cctctatcgg
     2521 cgttttccct tctttctgca tcatctcaac ggcaaattcg acaatcagga tggcgttctt
     2581 ggcggaaagc ccgatggtgg tcagcaaacc aacctggaag tagacgtcat tacttaagcc
     2641 gcgcagatcg gtggccagta atgcgccaac gacgcctaac ggaacaacca acatcaccga
     2701 gaacggaatt gaccagctct catagagtgc ggcgagggcg aggaacacca cgaccagtga
     2761 aatggcatac agtgcaggag cctggtttga ggataacgct tcctgatacg acagtccggt
     2821 ccatgagtag ccgacgcctg ccggaagttt agcgaccagg tctgccataa atttcatagc
     2881 gtcaccggta cttttcccgg ccgccgcttc acctaaaatc tccattgacg ggatgccgtt
     2941 gtagcgttcc aatcgcggtg aaccataggt ccattcggta gacgagtagg cagaaagcgg
     3001 tgccatcgtg ccagaggcgt tgcgtacata ccattggttg atgttatccg gcaacatacg
     3061 gaacggcgtg cctgcctgga catacacttt tttcacccgc ccctggttga ggaagtcgtt
     3121 cacgtagctg ctgccgaagg cggtggaaat tgtctggttg atatcagaca gcgccacacc
     3181 cattgcctca gctttcgcgg cgttgacgtt cactttgaac atcggcgtat cttccaggcc
     3241 gttcgggcgt accccggtga cctggtcagg cgattgcgct gccagtgata acagctcgtt
     3301 tcgtgcctgg gttagttttt cgtgccccag gttaccgttg tccagcagtt ccatatcaaa
     3361 acctgacgcg gtacccagtt cagccaccgc gggtaagttg aacgggaaga cgacggcttt
     3421 attgatactg cttaacgcaa tcattgcccg ctgaatgatc gcggtaaccg agttttcctc
     3481 accgacacgt tcagaccacg gcttgagact gataaacgcc aggccgttgt tttgcccctg
     3541 gccgctgaag ccaaagccgc caacggtaaa caccgactga acattatctt tctcttttgt
     3601 aagataataa tccgtcacct gttgcagcac tttcgtggtg ttaaccatgg tggcaccgga
     3661 aggtaactgc gcggtggtca taaatacccc ctgatcctct tctggtaaga aagaggtcgg
     3721 tgtacgcagg aacagcaccg ccatcccggc gcaaatcagc aggtagacca ccatgtagcg
     3781 accggtacaa cgcaacagcg agcgggtgct atcggtatag tgctgagttg atttttcaaa
     3841 cagcgtgttg aagcgtgcga acagggcgtt aggtttgtga ccgccttccg gcgcggcttt
     3901 cagaatggtg gcgcacaggg caggggtcag gctcattgcc acaaatactg aaagcagcat
     3961 ggaggagatc agcgtgatgg agaactggcg gtagatctcc ccggttgcgc cgctcataaa
     4021 ggccatcggc ataaacactg cggaaagaac aacagcaata ccgaccagcg cacgttggat
     4081 ctgccccatc gatttatgcg tcgcttcctt cggtggtagc ttatcttccg caatgacacg
     4141 ctcgacgttc tccaccacca cgatggcgtc atccaccagt aaccctatcg ccagcaccat
     4201 cccgaacata gttaacgtgt tgatggtgaa accgaccgcc gacaagatcg caaacgtccc
     4261 gagaataacc accggtacgg caatcgtcgg gatgattgtg gcacggaaat tctgcaaaaa
     4321 cagatacatg accaggaaga cgaggatgat agcctcaacc agtgttttaa aaacttcctg
     4381 aatagaaatt tcgataaacg gcgtggtgtc gtaaggataa accgtcttca gacttgcggg
     4441 gaaataggct gataagcggt tcagctcctc tttgactgcc cgcgaggtat ccagggcgtt
     4501 tgctccggta gccagtttga tggcgatccc ggcagcaggt ttgccgttat agcgcgccac
     4561 ggtggaataa tcttccgctc caagttcgac gcgggcgaca tcacgcagca gcacttgcga
     4621 accatcttgc tgaactttca acaggatttt gccaaattct tccggcgttt gcagacgcgt
     4681 ctgcacaatg atcgaggcgt ttagttgctg gtctgccgcc tgtggcatgc cacccagttg
     4741 accaccggaa atctggttgt tttgcacctt aatctgggaa ataacatcgg aaggtaccag
     4801 attgtattta ttgagttttt gcgggtccag ccagatacgc atggcgtact cggaaccaaa
     4861 gagctgtacg ctaccaacgc ccgcggtacg gcttagcggg tctttgatat tagacgctac
     4921 atagtccgcg atatcgtact ggttgaggct gccgttatca gaaataaacg ccgctaccat
     4981 cagaatatta ctgctcgact tatcaacgct aatcccctgc tgctgcaccg cttcaggtaa
     5041 tgaaggcata gcgagttgca gtttattttg cacctgaacc tgtgcgatat caggagatgt
     5101 cccagtctcg aaggtcagag tgatagaggc attacccgcc gcatcactgg ttgaagacat
     5161 gtacatcagg ccatcaagcc cattcatatt ttgctcaatc acctgagtga ccgagtcttc
     5221 taccgtttgc gcatcggcac ctggataggt agcgctgacg gtaatggtcg gtggcgcaat
     5281 ctgcggatac tgcgcaaccg gtaagttcat gatcgccaga ccacctgcaa gcatcataat
     5341 aatggcaagt acccaggcaa aaaccgggcg atcaataaaa tagttagcca tgaaagtccc
     5401 cttaagcctg caacgttatt gtttcgattc ggtgctggcg ttttcctggc tggaggaaat
     5461 tgctcgtgct ttgatacccg gacgaatgcg ttgcaaaccg gaaacgatca cccgatcgcc
     5521 agcctgcaag ccagaggtga caacccactg gtcgccgatg gctttgctgg cttcaatttc
     5581 gcgtagctgc acaacatcgt ctttatccag aatgagcgcc gttgctttac cctgggcgtt
     5641 gtgggtgacg ccttcctgcg gcaccagtaa tacattctgg cggctacctt catccactaa
     5701 tgccgtgacg tacatgccag gcagcaagtc accatttggg ttggggaaaa tcgcccgtaa
     5761 cgtcacggag cccgtggttt catccaccgt cgggtcggag aatttcagcg tgccggtctg
     5821 gctgtagcgt ttaccatttt ccagattgag ctgtactggc gtactgccct gaacctgttt
     5881 gatttgccca ctggcgacct cttctttcat gcgtaagaaa tcttgcaccg actgcgtgag
     5941 atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct gattagcggt
     6001 aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg gcgaggtgac
     6061 attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc gctttggcga cggtgacatt
     6121 ggcttctgct tcattcaact gggtgcgcgc ggtatcgtaa tcctgacggc taacgtagtt
     6181 ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg cggtagagag
     6241 cgctttcgcc agcgagcctt tggcggagtt tagctcggcc tgtaaaggtg caggatcaat
     6301 ctgatacagc gaatcgccct ggttcacttt atcgccttcg ataaagttgc gtttaatgat
     6361 aataccgccc acctggggac gtatctcggc aacttcataa ggaacggttc taccgggcaa
     6421 ttcgctcaac acattgaccg aaccggggga gagtgtgacg acaccgacct caggcgtcat
     6481 ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc cgcagaataa
     6541 caacggtatt aacagctttc ttcttctgtt cattttagtc cctgaaaatt cttgagaaat
     6601 aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc agcggcatag
     6661 tggggttgtc agccagtaag gtgggataca ggcacagtga tcgacatggt gaggtcaacg
     6721 acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag cattcgggca acgaagggtt
     6781 ctactggtgg atacacatac caggggaata aagtcgtttt agcctctcct ttagtaatca
     6841 ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa tttcagttgc
     6901 ttatgtcctg actaaaaata agatgtgata cccagggtga cgatgtcgct catacgtctt
     6961 agtttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg cgaaatattg
     7021 agcgtcgacg tgatattgct ttcattttta tttttcagaa tcagttgcat aattttgaac
     7081 tgattat
//
LOCUS       DAZFRJ010000207         7038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000207 DAZFRJ010000000
VERSION     DAZFRJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.209"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            243..1013
                     /gene="yjjP"
                     /locus_tag="ACHKUN_004043"
     CDS             243..1013
                     /gene="yjjP"
                     /locus_tag="ACHKUN_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO6662202.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1004..1477
                     /gene="yjjB"
                     /locus_tag="ACHKUN_004044"
     CDS             1004..1477
                     /gene="yjjB"
                     /locus_tag="ACHKUN_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO6662203.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1584..2123
                     /gene="dnaT"
                     /locus_tag="ACHKUN_004045"
     CDS             1584..2123
                     /gene="dnaT"
                     /locus_tag="ACHKUN_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO6662204.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2126..2863
                     /gene="dnaC"
                     /locus_tag="ACHKUN_004046"
     CDS             2126..2863
                     /gene="dnaC"
                     /locus_tag="ACHKUN_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO6662205.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2912..3406
                     /gene="yjjA"
                     /locus_tag="ACHKUN_004047"
     CDS             2912..3406
                     /gene="yjjA"
                     /locus_tag="ACHKUN_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO6662206.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3660..5951
                     /gene="opgB"
                     /locus_tag="ACHKUN_004048"
     CDS             3660..5951
                     /gene="opgB"
                     /locus_tag="ACHKUN_004048"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO6662207.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6090..>7038)
                     /gene="lgoD"
                     /locus_tag="ACHKUN_004049"
     CDS             complement(6090..>7038)
                     /gene="lgoD"
                     /locus_tag="ACHKUN_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO6662208.1"
                     /translation="GDNEALIKIKTVGICGTDIHAWAGNQPFFSYPRVLGHEICGEII
                     ESGKNFHNFNIGQQVAVIPYISCQQCPACKSGRTNCCEKISVIGVHQDGGFSEYLSVP
                     VANILPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAAIAKAD
                     GAQVVVADTSPARREHVATRLELPVLDPSAEDFDAQLRAQFGGSLAQKVIDATGNQHA
                     MNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDFAKVGRLMAE
                     GKITADMMLTHRYPFATLAETYERDVINNRELIKGVITF"
BASE COUNT         1730 a         1851 c         1766 g         1691 t
ORIGIN      
        1 cggttatcta ttatcctgat tcgtaaaggc caaacaataa attcattatg atgataatat
       61 tcatgtgata gtttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttctcccg
      121 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca
      181 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      241 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
      301 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      361 cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacca
      421 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      481 atgtggtgac tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca
      541 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
      601 ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg
      661 gtgccgtcat caccttcttt gccagtacga ccgcgatgta tatccgccag ctgctggcac
      721 aacgtcatct tcatccacag atcaactttt gccttaccgc tttcgccgcc accaccattt
      781 ccggattgct tttgcaactc cccactttca gcgatacacc caccattgcg atggccgcca
      841 gcgttctgct gctggtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag
      901 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgacg ctggctacct
      961 gcgtcggcgt ggtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
     1021 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
     1081 ggtgttcaac gttcccgttc gggcgttacg ctggtgtgcg ctgcttggcg cgattggtca
     1141 tggttcacga atgatcttaa tgaccagcgg gctgaatatt gagtggtcaa cttttatggc
     1201 ttctatgctg gtcggcacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
     1261 agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat
     1321 gatttcggcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga ttaccctgtt
     1381 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
     1441 tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg
     1501 atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca
     1561 tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta
     1621 ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg
     1681 ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac
     1741 tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg gacgatcaac
     1801 tctatcagga accgcaagcc gctcccgttg ctgtccccat ggggaaattc gccatgtatc
     1861 cggactggca acccgatgcc gactttatcc gcctggcggc gctatggggc gtggcgctaa
     1921 gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta
     1981 aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg
     2041 ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagcgaacct gacagccaaa
     2101 ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca
     2161 aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg
     2221 gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa
     2281 aatgcagcgc acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga
     2341 gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt
     2401 cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa
     2461 aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat
     2521 catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcggtaccag
     2581 cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg
     2641 cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc
     2701 ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt
     2761 gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa
     2821 ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt ttctggcggt
     2881 atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac atcttctgtg
     2941 ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga
     3001 tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg
     3061 ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg aaccaggcct taagcgcaga
     3121 taacatgaac aacgccgcag gcattctgca atactgcgcg aagcaaaagc tggcgtcggt
     3181 aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga
     3241 acaaaaagaa gacaccaact atctggatgg cattcagggt ttgctgaaaa ccaaagatgg
     3301 tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg tgaaaaccaa
     3361 agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaacgccg
     3421 cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg
     3481 gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta taaactaatt
     3541 cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat
     3601 tgtgcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccgt
     3661 tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag
     3721 cgggacgtaa cacctggtgg tttgcagcca cattaacggt gctggggcta tttgtcgttt
     3781 taaatatcac cctgtttgcc agcgactatt ttactggcgc tggtattaac gacgcggttc
     3841 tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc ctgccgggta
     3901 tcggcattgt gctggggctg acagcggtgt tcggtgcgct gggctggatc ctgcgccgtc
     3961 gtcgccatca tccgcaccat tttggttaca gcctgctggc gctcttactg gcgctgggtt
     4021 cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg
     4081 atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga
     4141 aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgaag
     4201 ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc
     4261 acacgcagca gctgccggga acagattaca cgattgcggg catggtggct tctcagtgcg
     4321 gcattccgct ttttgcccca tttgaaggta acgcctccgc ttctgtttcc agcttcttcc
     4381 cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc
     4441 agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat ggcttcgacc
     4501 acttatacgg ctcagaagag ctgaaaagcg tggtagccga cccgcactat cgcaacgact
     4561 ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa gagctttccc
     4621 gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt
     4681 ttatctcgcg tacctgtaac cgcaaaaaat atgattttga cggtaagccg aaccagtcat
     4741 tcagcgcggt aagttgcagc caggagaata tcgcggcgtt tatcaacaaa atcaaagcgt
     4801 caccgtggtt taaagatact gtcatcgtcg tctcttctga ccatttagcg atgaacaaca
     4861 cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc attcgtggcg
     4921 acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac ggcgcgacgg
     4981 tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc
     5041 agtcaatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccggata
     5101 tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa
     5161 acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca
     5221 aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc cagctggccg
     5281 atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctacaagatg gcacaactct
     5341 gggctccgga actggcactt tccaccgact ggtgtgtctc gcaggggcag cttggcggtc
     5401 agcaaattgt tcagcatgtt gacaaaacaa catggcaggg caaaacggca tttaaagata
     5461 cggtcatcga catggcgcgt tacaaaggca atgtcgatac gctgaagatt gttgataacg
     5521 atattcgcta caaagccgac agtttcatct tcaacgtcgc cggtgcgcca gaagaggtga
     5581 aacagtttag tgggatttcc cgtccagagt cgtggggccg ctggtccaac gcgcagctgg
     5641 gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta
     5701 ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag
     5761 aacaaaccct tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa
     5821 ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata
     5881 tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gttgtggaac
     5941 gtgaagggta attcctgact gatgtgtagg tcgcataagg tgcgacctac acaccttctc
     6001 cggcctacaa aacatcattg agaaacagaa aaaaacagcg cccaacactt ccccggacgc
     6061 tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccttt aattaactca
     6121 cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg atagcgatgg
     6181 gttaacatca tgtcagcagt gatttttcct tccgccatca gtcgaccgac tttagcaaaa
     6241 tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt cttttttatg gaattccgga
     6301 tcggagaact gcaactcacc tttaaacagg ccgacaaata ccaccgtgcc gccgtgacga
     6361 atcagattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6421 gccagcgaac caccaaactg cgcccgcaac tgcgcgtcaa aatcttcggc tgacgggtcc
     6481 agcacgggca actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6541 accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc
     6601 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6661 ctaatagcga aaggttcaat caatgcggcc gcctgcgggt caataccgtc tgcgggcaaa
     6721 atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6781 actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca ttgctgacaa
     6841 gaaatatacg gaattaccgc aacttgctgt ccgatattaa aattgtgaaa gttttttcct
     6901 gattcaataa tctccccaca tatttcatgg cctaaaacgc gtggatagct aaaaaatggt
     6961 tgattgcccg cccaggcatg aatatctgta ccgcaaatcc cgacggtttt aattttaatt
     7021 aatgcttcat tatcgcct
//
LOCUS       DAZFRJ010000208         7029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000208 DAZFRJ010000000
VERSION     DAZFRJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.210"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..1405
                     /gene="adeQ"
                     /locus_tag="ACHKUN_004050"
                     /gene_synonym="yicO"
     CDS             71..1405
                     /gene="adeQ"
                     /locus_tag="ACHKUN_004050"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO6662209.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1458..1910
                     /gene="yicN"
                     /locus_tag="ACHKUN_004051"
     CDS             1458..1910
                     /gene="yicN"
                     /locus_tag="ACHKUN_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO6662210.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2121..3311
                     /gene="nepI"
                     /locus_tag="ACHKUN_004052"
     CDS             2121..3311
                     /gene="nepI"
                     /locus_tag="ACHKUN_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO6662211.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3352..3645)
                     /gene="yicS"
                     /locus_tag="ACHKUN_004053"
     CDS             complement(3352..3645)
                     /gene="yicS"
                     /locus_tag="ACHKUN_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO6662212.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            3867..4685
                     /gene="nlpA"
                     /locus_tag="ACHKUN_004054"
     CDS             3867..4685
                     /gene="nlpA"
                     /locus_tag="ACHKUN_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO6662213.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4689..5612)
                     /gene="yicL"
                     /locus_tag="ACHKUN_004055"
     CDS             complement(4689..5612)
                     /gene="yicL"
                     /locus_tag="ACHKUN_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO6662214.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5723..6907)
                     /gene="setC"
                     /locus_tag="ACHKUN_004056"
     CDS             complement(5723..6907)
                     /gene="setC"
                     /locus_tag="ACHKUN_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO6662215.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1758 a         1587 c         1775 g         1909 t
ORIGIN      
        1 tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat
       61 ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag ggacgctttc
      121 gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat
      181 gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc
      241 acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat
      301 tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg ggctgaacgc
      361 cttctttgcc ttcgtggtcg tgggggcgat gggcatctcc tggcagaccg ggatgggcgc
      421 aatattctgg ggcgcagttg gactattttt gctcacgctg tttcgtatcc ggtactggat
      481 gatctccaac attcccttaa gtttacgtat tggtatcacc agcggaattg gattatttat
      541 cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca cgctggtgat
      601 gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac
      661 cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt tctattgtgg tgacgtcttg
      721 ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat
      781 tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat
      841 cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac
      901 tgataaagcg ggcttaatag atagtaacgg taaattcccc aatatgaata aggcgctgta
      961 tgttgatagc gtcagttcgg tggcgggggc gtttatcggc acctcgtctg ttactgccta
     1021 tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctgactg cggttgtggt
     1081 tggcgttatg ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta
     1141 cgcaaccgcc ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt
     1201 taactgggat gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt
     1261 tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt
     1321 atgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc
     1381 actgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg
     1441 attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg
     1501 ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agcgatcggc
     1561 tgaacatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagctggtg
     1621 acgaattttt acgttatttg catcgtccgg atgagtcgca cctgcaaaac gccgcgcagg
     1681 tgttgctcat ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc
     1741 atcggatttt acaaaaagct cgcctcgccg cgccgattac cgacgctcag gtcaggctgg
     1801 cgctaggttt tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc
     1861 gctataacgc gttctttcag cctgccgagg gcgttcactg gttgcattga aaccgtttcg
     1921 tcttatcagc ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc
     1981 cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag
     2041 ttgcaaaaac gttaaatgcg tcacacattt caatgttaaa gtggtcggct tttcccctga
     2101 aacatgccac gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca
     2161 tcacccgacc gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta
     2221 tcgttgagtt tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg
     2281 aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt
     2341 ttattaccca gacaattcag gctactgacc gccgctacgt tgttattttg tttgccgttt
     2401 tgctgacgct ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg
     2461 gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca
     2521 tgcgtctggt gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt
     2581 ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc
     2641 gcaatgtctt taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat
     2701 cattgccttc actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac
     2761 aacgtccggg tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg
     2821 ctttcttcac atatattcgc ccggtgtata tgaacctggc gggattcagc gtggatggct
     2881 taacgctggt gctgttgagt tttggcattg ccagctttat cggtacgtcg ctttcgtcat
     2941 tcattcttaa acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga
     3001 gtgcgttggt gctgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta
     3061 tctgggggct aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc
     3121 tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata
     3181 cctgtggcgc ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga
     3241 tgttgtccgg cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga
     3301 agaaatcctg atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc
     3361 cgggcattct accttaccca acgcttccca ataattgctc tgattattac gttccatcgc
     3421 cacgatggcc tcgtgtatat gttgtttatt gttttcatct gacaacatta acttttccca
     3481 cgcttcatct gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt
     3541 ggttttctct tttgcggact gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg
     3601 catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta
     3661 caaatgtgta tcgaatgaag cataagtgta gtttgctttc atcgctctca tcatgctgta
     3721 cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt
     3781 tttattctta tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag
     3841 gtaatcatta ataaaaggat aaaaaaatga aactgacaac acatcatcta cggacagggg
     3901 ccgcattatt gctggccgga attctgctgg caggttgcga ccagagtagc agcgatgcaa
     3961 aacacattaa agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa
     4021 aggtggcaaa agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc
     4081 taccgaacga tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag catcgaccct
     4141 ttcttgaaca agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg
     4201 tcttccctat ggcgggttat tccaaaaaaa tcaaaaccgt ggcgcaaata aaagaaggcg
     4261 cgacagtggc gatccccaat gacccgacca accttggtcg cgcactttta ctgttgcaaa
     4321 aagagaagct aattactctg aaagagggaa aagggttatt acctaccgcg ctggatataa
     4381 ccgataatcc tcgtcatttg cagattatgg aactcgaggg ggcgcagctc ccgcgcgtac
     4441 tggatgatcc caaagttgat gtagcgatta tcagcaccac ttacattcag cagacagggc
     4501 tttctccggt gcacgacagc gtatttattg aagataagaa ttcgccgtat gtgaatattt
     4561 tggtggcacg ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc
     4621 aatcacccga agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct
     4681 ggtaattctc actcatgccg cgccggacga tttatttttc tggcgcggcg acgggaatcc
     4741 attgaaatca aaattaccga tgacagaatt agcagcgttc ccagccagtc cggtaatgtg
     4801 aacgtgatcc ccagcaacag caaagagagt agcgcgctac ttaatggttc tgcacagctc
     4861 aaaatgctgg cttttggacc gccaattaat tgtgctcctt tcaggtacag actaaatgtc
     4921 agggacgtac caatgacgat caaataaaaa aacgccagaa tcaaactgcc gttaacgaca
     4981 aagtttgttc cttgtctggc ataaaaaggc aacagaatca gaccgccaat cagcatactc
     5041 cagccgacga ctggtaacgt gccatagcgg gcaattagtg ttgagggata ggtggtatag
     5101 aatgcagcag caaatgccga ggcaatgccc cagaacaacg cggcaggaga gatcgataat
     5161 gacgtcggat taccgtgtgt caccaataaa aaagtcccga ccagcgatgt caaaatagcg
     5221 cagaaaacca gaatgcccgg gcgcgattta cgcaccagtg agaaccaggc gacgataatc
     5281 gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt tcgatttttc gatggttagc
     5341 aaaaaagtga gctgtacagt tagcgcgcca accacggaaa aaatcagcag gctaatggca
     5401 tctttatgat tgttaataat agaaaagatt ttatcgccat gaacaaatga cagcgtcagt
     5461 agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa actgcgacga catctggctt
     5521 tgctccatga tgtattgcgc acagacccct gaacttcccc acaacacggc ggcaatcaga
     5581 acgttcagca tcccctttct ggtggaaccc atttttccct ctgcatactg ttttgtcgtt
     5641 tatgtatgca ggcatgatag caaaaatggc gaggatggct ctgtttgcgc cacgccgatt
     5701 tttcttagcc cagactggtg agctaaatat ctttaataaa cagcaggcaa atcatcgcaa
     5761 tacccaacat ccctatcgcc agccagaaca acgcatggta gctccagatt tcaaccataa
     5821 ttccgtcaac agagccggcg ataatccagc cgacgcgtga agtatttgca tataacgttg
     5881 tcgcagagcc tattttttca ggcatcaagt cctgaaaata aagcatgccg ataccacaga
     5941 gaataccaag gaagatggca ttaagaattt gcaattccag ctcaaccgcc ggagtcgtcg
     6001 ccatgagtac gctggcgtaa aaacacattc cactcacgat agcaatgagc attaatagtc
     6061 gcttgccaat acgtttcatg taatagcctg cgattaacat catcggaatt tccagaccgg
     6121 cagcgataca aatcatttct ccagccagtt tatcggttag atgcagttca tcaataataa
     6181 atagcggcat atttatcatg tagagattat tcgccgccca catcattgaa cagaccacaa
     6241 aaagtagccg cgtatcccgc ttcctgtggg tggagggtaa aatttctacg ggttgggtaa
     6301 cgacaggaat atttctttgt attgatggca aaaacaacca gactatcagt ccgcaaacaa
     6361 caaatgcgat ggcagcggtg agatacatca ctttaaaact aaatcccatt gccagttcat
     6421 aagcgagcgg tggcccgata acccaggcaa gcgagatctg agcacgtaaa aatgtactga
     6481 acatgaccgt ctcacggcct gttctgtcgg cgtgttcacg ggcgagggcg aacatttgcg
     6541 ggtttgcggt ggaagcaaaa ctactcagaa gtacgcccgt tgagaggaga atgaagtagt
     6601 tgcgattcca cgcaaaaagc gtgcaggcca gcactccaaa taagcaacat agcagaatca
     6661 gtaatttacg gtcgccttgt ttatcggagt gccttgccag aaattgactg accagaattc
     6721 ccataatagc gctaccggtg aagaaaaaat ctaccattat aggacgggct ttcagttcat
     6781 ctccgaggaa tatacttagg gtaggagtct gaagagcgcc cgcaataccc gtcagaaagg
     6841 cgacaagtaa aaatgccgcg gcagtgagat caagtatttt tgatggagtg gtagccgttt
     6901 tttgcattct tctgaatcac cgggcgacaa aatataactg agttacaaat aagtaccagt
     6961 cgaatgagta ttgtattaag aatttttgag tatcgctgat acaaccagaa agcaatgcta
     7021 gcgcaatat
//
LOCUS       DAZFRJ010000209         6995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000209 DAZFRJ010000000
VERSION     DAZFRJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.211"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            281..1408
                     /locus_tag="ACHKUN_004057"
     CDS             281..1408
                     /locus_tag="ACHKUN_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO6662216.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            1414..2205
                     /locus_tag="ACHKUN_004058"
     CDS             1414..2205
                     /locus_tag="ACHKUN_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO6662217.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(2241..3166)
                     /locus_tag="ACHKUN_004059"
                     /pseudo
     CDS             complement(2241..3166)
                     /locus_tag="ACHKUN_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(3311..4660)
                     /gene="lysC"
                     /locus_tag="ACHKUN_004060"
     CDS             complement(3311..4660)
                     /gene="lysC"
                     /locus_tag="ACHKUN_004060"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO6662218.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(4752..4948)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5185..6834
                     /gene="pgi"
                     /locus_tag="ACHKUN_004061"
     CDS             5185..6834
                     /gene="pgi"
                     /locus_tag="ACHKUN_004061"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO6662219.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1777 a         1681 c         1821 g         1716 t
ORIGIN      
        1 actgaattac aaagggggtc aaaccccgtc ggcacaccag gaacggccag gctgttttcc
       61 gggcgtgttc cgtcagcctt tgacgccaat tatgggttcg attcccacat tcattcctga
      121 aatgaatact ggttttaact tattaaataa aaagtgaggg attgcgcagt cagcgatccc
      181 cgataacgtc gccgataaag atgccgggta acaggcaaaa gggaattccc ctgtcgcttc
      241 gtgtctgctt ccgcgacggc tgttaagcaa ggaatagata atgattaaaa aaataagcgt
      301 aagaaaagat cagctggcat tactctccag aaacggcgat tactacaaag tgctacatgc
      361 aggcgagcat cttttaccct ggttaaatac gccagaagtc ttgctgatta cgctcgatgg
      421 cagcgaagtt cccgacgtgc tggcggatta tttgcgccgc tttcagcctg attgggtgga
      481 gaaatattgc ctggtggcag acttgtcgga gattgaagcc ggggcgttgt atatggacgg
      541 tattttgcag gagatcctgc cgccatcaac gcgccgtttg tactggcgtg tagaggatga
      601 tttgacactg gtgcggatga atacgcagca agtccaggta caaacggagg tgatgaatgc
      661 ggtattgcaa ccacggcgca aaggggcagt taaaggccgc gatgcaatac taacggtgca
      721 ggttccggcc tggcatgtag gcgtgctgaa aatcgatggt gaaacgcagg cgctgttacc
      781 gccggggctg accgcatact ggaaaattaa ccatctggtt gacgctgaag tggttgacac
      841 ccgcttgcag gtactggaag tcagcgggca agaaatctta acgaaagata aggtcaatct
      901 gcggattaac ctcgccgcta actggcgcta cagcgacgtg ttattggcat ttagccagtt
      961 aacgaagccg attgatcatt tgtatcggga attacaattt gccctgcgtg aagcggtagg
     1021 tacgcgtacg ttggatgaat tgctggaaga taaacaggtc attgatgacg ttgtcagcga
     1081 acaggtgaaa tcgcgcatgt tgccatttgg tatggagatt gcctcgttag gggtgaaaga
     1141 tatcgtcctg cctggcgata tgaaaaatat ccttgcgcag ctggttgagg cggagaaatc
     1201 cgcgcaggcc aatgtgattc gccgacgtga agaaacggca gcgacgcgtt cgttactcaa
     1261 taccgctaaa gtcatggaaa acaacccggt agcgttacgc ttaaaagaac tggaaacgct
     1321 ggagagagtg gctgaacgga tcgataacat ctctgtattt ggcggtctgg atcaggttct
     1381 gcatgggttg gttaatatca aagggtgagt aacatgccat taaatggagt cagtgctgcg
     1441 atgcgagaaa gggtatcgca gcagttaaaa gaaattgaac ggcggtatga tgtgaaagta
     1501 ctgtacgcct gtgaatcagg gagccgcggc tggggatttg cctcgcccga cagcgactac
     1561 gatgtacggt ttttgtatgt tcacccgctg gaatggtatc tgcgagtaga gtcaccgcgc
     1621 gatgttattg agctgcccat cgacgacgag ttagatgttt ccggttggga gtggcgtaag
     1681 gcgctgggat tattgaaagg cgcgaacccg acgctgatag aatggctgga ttctccggtg
     1741 gtttatcaac aagatgaaga aacgataacg gcgtttaagg caatggtccc aatgtggttt
     1801 tctccgctgc gtgcgcgctg gcattactat tcgatggcgc agaaaaactt ccccggatat
     1861 ttgcagggcg acgaagttcg actgaaaaaa tacttttacg tgctgcgtcc gctgctggcc
     1921 gtgcgctggg tcgaagcggg taaaggcgta ccgccaatgc gctttgctga gttactggcg
     1981 ggaagcgaac ttgatgcgcc gttacgtgca gaaatagacg aattactgga acgtaaacag
     2041 cgcgcaggag aagcggaata tggtccgcgc cgtccattgt tacatgcgtt tattcgtgcg
     2101 gaactggcgc gtggggaaat tccaccgctg ttaccggata gcagagaagg ggacgttaag
     2161 gaactggata gcttaatgta ccagacggta atgcgccggg cataaagccc ggcgttattt
     2221 cacgccatca gctcttatta ttattgtgta ttcccgctga tttcattctg tataagcgtt
     2281 gcaatatcgt cattcgttgc cgcttgtgaa ctaaatatca tcttctcacg cgaatacacc
     2341 gcaaacttat ctgacaggtt cgaagcaaac accttgcggg cattttctgc cagcaaaccg
     2401 gtgacgatat catggtggtt tttatccact tttcgggcaa tatttcccgc ctctttgtcc
     2461 ttactatacc gttcattggt gctttcccag cttaatgccg ctgaggttgt ttttatcatg
     2521 acttctgttt ggtagcctgc atctttgagt tttttaatgt ttgtaacggc gtcgcggcat
     2581 tacgaaatgt tccttcgaca acaatgttga gtttactgac tatcgctctc tcaattgcac
     2641 gttcgaccgt ttcgccggaa aatttcgctg tatgggtaac aaaatcatcc ccataatttt
     2701 ggtagatata attaaaatca gggtgtagaa atcgaaaatc atcaccgtta ataatcatta
     2761 tattatcttt tgtctctctt ttggctaatt caatcagaga tgatttacct gctccgggtt
     2821 gcccacctaa aacaaaacca atgggatgct gttgcggtgt gacttcatga tttgtcagac
     2881 gctgccaaat agaatcaaaa gatgctttat tttctgaatg cgtagtattg ttgaattcat
     2941 gctgatattg agttcttacc tgctgaagga gaatcttacc ttgtgtaaaa tcaacaagtt
     3001 cagcgggggt agaataaaga taatcgcgat agctgactaa tcgcttgaga gcatcgcctg
     3061 aatcctgatg cgacgaaact gagcaattat caatgatatt tgataaaaac gcgatgcact
     3121 gactggctcc gtttaaaagt gcatcttttt ccattaaagt agccattaac actcctgtta
     3181 aatgatattt ttcggtgatt ataatactgt tctacaggag tgattttgct tttcagatat
     3241 tatttaatct atgttttcag tgaggatggc gtataacaat cggtgagcgt tatacgccag
     3301 tagggaaata ttactcaaac aaattaaaat gcagcttctg caccacctgc tcggcatctt
     3361 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
     3421 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
     3481 tgccaatcaa cgcgaccagc gccagacctt cttctacttc cacccggcac agtgcggaaa
     3541 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
     3601 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
     3661 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
     3721 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
     3781 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
     3841 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
     3901 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
     3961 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
     4021 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
     4081 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
     4141 gacgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctatatct ggctctgcac
     4201 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     4261 cgcgttcgcg caggatctca acaaacagca gggtcgacat cagctcgccg tggctgacca
     4321 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     4381 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     4441 tggcaaactg gatgttgcgg atagcgtcga gtttttcgaa tcgctcgcca ggttccagtc
     4501 cttcagctaa agcgaccagc agattagtga taccagcaga agccgagagg acaactaaac
     4561 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     4621 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     4681 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     4741 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     4801 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     4861 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     4921 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     4981 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     5041 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacgctaa
     5101 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     5161 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     5221 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     5281 aaagacggcg atcgtttttc taagttctcc gcaaccttcg gcgatcagat gctggtggat
     5341 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaagatct ggcgaaagag
     5401 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     5461 aaccgcgctg tgctgcacgt agcgctgcgt aaccgtagca ataccccgat tttggttgat
     5521 ggcaaagacg taatgccgga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     5581 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     5641 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     5701 aaaaatcacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat cgctgaagtg
     5761 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     5821 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     5881 gaaaaacacg ttgcgaaaca ctttgcggcg ctctcaacca atgccaaagc cgttggcgag
     5941 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     6001 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     6061 ctgctttctg gcgcacacgc gatggacaag catttctcca ccacgcctgc cgagaaaaac
     6121 ctgcctgtac tgctggcgct ggttggcatc tggtacaaca atttctttgg tgcggaaact
     6181 gaggcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     6241 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     6301 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     6361 caccagggaa ccaaaatggt accgtgcgat ttcatcgctc cggctatcac ccataacccg
     6421 ctctctgatc atcaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     6481 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     6541 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     6601 ctgctgcgtg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     6661 atctttactc agggcgtgat tctgaacatc ttcaccttcg accagtgggg cgtggagctg
     6721 ggtaaacagc tggcgaaccg tattctgcca gagctgaaag atggcaaaga aatcagcagc
     6781 cacgatagtt cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatcg
     6841 tcgatatgta ggccggataa ggcgttcacg ccgcatccgg caaccgatgc ctgatgcgac
     6901 gcggtcgcgt cttatcaggc ctacaggtcg atgccgatat gtacatcgta ttcggcaatt
     6961 aatacatagc acgattgatt aaataacctt aataa
//
LOCUS       DAZFRJ010000210         6991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000210 DAZFRJ010000000
VERSION     DAZFRJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.212"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(6..1115)
                     /locus_tag="ACHKUN_004062"
     CDS             complement(6..1115)
                     /locus_tag="ACHKUN_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO6662220.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETA"
     gene            1273..2457
                     /locus_tag="ACHKUN_004063"
     CDS             1273..2457
                     /locus_tag="ACHKUN_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6662221.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HDQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            2457..2969
                     /locus_tag="ACHKUN_004064"
     CDS             2457..2969
                     /locus_tag="ACHKUN_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6662222.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            3025..3399
                     /locus_tag="ACHKUN_004065"
     CDS             3025..3399
                     /locus_tag="ACHKUN_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662223.1"
                     /translation="MKDIDTETRNNTVADDVTAGEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            3408..3563
                     /locus_tag="ACHKUN_004066"
     CDS             3408..3563
                     /locus_tag="ACHKUN_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6662224.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3550..6357
                     /locus_tag="ACHKUN_004067"
     CDS             3550..6357
                     /locus_tag="ACHKUN_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6662225.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGILKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAVGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            6370..6858
                     /locus_tag="ACHKUN_004068"
     CDS             6370..6858
                     /locus_tag="ACHKUN_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662226.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
BASE COUNT         1567 a         1756 c         2055 g         1613 t
ORIGIN      
        1 gacattcacg ctgtttctgc tatccattcc gggatttttg cctcaagctc aagctgcgtg
       61 gtaaagccgc tgttatcaat ggtgtgctcg gcttttgcaa taatccagtc ctgattatca
      121 atctcgcttt taaatcctgt taccgtgcca tgcatttcgg ggtagagttc tgcacgtcca
      181 cgcgccagcg tgatggaaaa tgatgcggct ccgcgttgta gctgctgcca ctttgccgcc
      241 gctgcgcgtc ttgctgcctg ctcgttctga taagtcttgc gtaacacaaa cacattgcct
      301 tccgcgcctt ccatataatc accttcacgg ctgctgcttt tctccttttt gggttttggc
      361 ggtttgcggc gtttcacgct gacttttttc tttttcccgt aattaagatc aagccagtaa
      421 gcgcgtaccc ccgtatacgc ctcgcggtca gcaatgcgga actgatggcg atcgccgctg
      481 ctgcgtgtga tggcgaacga tggcaacggc tggccctgtg cgttcacgcc accacctggc
      541 atgatgaata acagattgcc gctttttacc gtggtgattg cgcccagcat ttccgccatg
      601 cgcgtaagga aggacatatc gctttcttcg gtctggtcgg cgtggtcgat ttcgatatcc
      661 atcagcattt cgctgatttg cggtttcaga ccataccgat gagcgatggc ggataccacg
      721 cgctcaacgg tcacatcatg ccaggacacc tcacgtttaa cgttaaattc atcccgaaaa
      781 tctgcgcttc tggctgaaac agtcagcctg tccggcggtc cttcgtgagc gatttcatca
      841 acaatgtaag tgcctttttc tgtcagcggt tctcctttcc agccaatgag aaccgtcagg
      901 cgcgcgcccc gtggcggtag ctgcaactga ccatcagcat catccagcgt gatggtgagc
      961 tggtccgcct caaatccccg gttgtcggtc agtgacaggc tcatcaggcg ctctgccacg
     1021 cctgacagcg ttttaccctc cgcgagaata tcaaaatccg gcattttcac ggggtctgtg
     1081 ccctgactga gcaattgcat ggtggtgtcg gtcatctgtt ccctccctgt gcggcatggt
     1141 cgcatgtgcg tgcggagggg gttactgctt tttgttgtcg ccgtggtggg agaacggcgc
     1201 aggggtgaga ttacgcgcgt ggtgggtgat gattgttgcc gaatcattta acggatacaa
     1261 ggggctgaag ctatgagtga aactcgtttt catggtgccc gtgttacgga aaataccgac
     1321 ctggtaacag cgattaacga tgttgattcc agcgttatcg gtatcgtggc aacggcggat
     1381 gatgcggacg cgaagctgtt cccgctgaac aagcccacac tgctgacccg cgttaatgac
     1441 gtgctgggaa aatgcggaac aacggggacg ctttatcgtg cgcttaaggc catcgcagac
     1501 caggtgagca caaaggtgat cgtcgttcgc gtggctgaac acaaagaaga agacggaaag
     1561 acgcaggatc aactggttat cggtggttct gaatctgacg gcagctatac ggggatgtat
     1621 gcgctgcttg ttgcagagca ggatgaaagc atcggatacc gtccgcgtat tctggccgcg
     1681 ccggagctgg acacggaggc ggtaacaaaa tccctgtgcg tgattgcagg taaactgcgc
     1741 gcgtttgtgt atgcctcatg tcacggctgt aacacgatgg ctgaggcgat tacctaccgc
     1801 cagaaattca acgaacgtga ggtgatgctc ttatggccgg acttcatcgc ctacaacccg
     1861 aaaagtggca aaaacgaaac gttccccgcg cctgcctatg cgtgcggcct tcgtgcgtac
     1921 attgaccatg atcaggggtg gcacaaatca ctgtccaacg ttccggttaa aaatgtgctg
     1981 gggatgtcga ggcatgtgtt ctggtcgttg caggccgaag acagcgatgc caacagcctc
     2041 aacaacaaag aaatcacgac cattattcgt cgcaacgggt tccgcttctg gggcaaccgc
     2101 acaccggaaa cgaacgccta catctttgag gtgtataccc gaaccgcaca ggtgctggct
     2161 gattcaattg cggaagcgca gtttgaaact atcgacagtc cactgacgcc tgcgaacgtg
     2221 aaggatgtta tcagtgccat cagggcaaaa ctggattcgc tggttactgc cgggaaactg
     2281 attggcgcgg agtgctggta tgacgtggtg gataacggca ccacgaattt acgtcaggga
     2341 cgtgtgcgta ttcgctacaa atatacgccc gttccgccac tggaagacat ggagctttac
     2401 cagacgttta ctgatgaatt ctttggtccc gcatttgcgg tgctgggagg tgcctgatgg
     2461 ctgtaccaaa acatcttcgc ttttttacgt tgtttgtgga tggtgaaaac gaagtgggca
     2521 aagtgacatc cgtcacgccg cccaaactga cgcgcaaaac cgacagctat cgtggtggtg
     2581 gcatgatggg ggcggtaagt attgatctcg gtctggacga ctccgcgctt gatgcgagtt
     2641 ttgtcatggg gggagctgtt cgtgcgctgt tccttaaata tggtggcacg attgacggca
     2701 cgctgctgcg ttttgcgggt gaatactaca ccgatgcaga aagcgatctg tatgaaatcg
     2761 agatgcgcgg gcgtgtgacg gaaattgata tgggggaagc caaacagggc gaagccacat
     2821 cacacactta cgctgtcaaa aacacctact acaagctgag cgttaacgat cgcccgctgt
     2881 gggaaatcga cctgttgaac cacatctacc ggaaggacgg caaggacatt gtgcctgacc
     2941 gtatccgttc cgcgcttggg cttggctgat aagtaatatg caggcggcgc agtgcgtcgc
     3001 ctctgactga aaaggagaca actgatgaaa gacatcgata ctgaaacccg gaataacacc
     3061 gtggcggatg atgtgacggc aggtgaggat atggctgtcg aacgtggcgt aaaactgacc
     3121 cgaccaattg agcgtggtgg cgaaaaaatc acgtatgtgg agatcaccgg ggctattgag
     3181 caggctggat ccctgcgtgg tctgtcgctg tctgatgtgc tgaatctgaa agcggatacc
     3241 atgtttacgc tgttgcctcg cgtgacctcg ccacgactgg atgaagtgat gattaaaaaa
     3301 atgtcgtcac gcgattttat tcagttgtgc gctgtggctg taaattttat gagcgagcca
     3361 gactctggcg cgaagagcgt gcaggagacg gcagcgtaat caccttggtg tgctttgagc
     3421 acatcgaaga tctggtggcg gatattgccg ccatttttaa ctggtcgccc gccgaaatct
     3481 tcatgatgac gcccggcgaa gtggttagct ggcgcgagcg ggcggcactt cgcagcggga
     3541 atgcagataa tgaagactct tgatatccgg gtcgctttca gcgccgttga caggctgacc
     3601 cggcctgccg aaaacgcccg ccgcctgatg gggcagtttg gtgactccat ccagcgaacg
     3661 cagggggcga tcaaaaatct cgagcgtcag gcgcgttcat ttgagcgcgc ccgtgacgct
     3721 gtcagtaaag cggatgctgg catattgaaa gcacgacgcc agcttaacgc tcttaatcag
     3781 ttacaacgca cgggtacagt gctcagcgaa aaacaacaaa agctgatgca gcagttaagc
     3841 acccggcttg aacgcctgaa tgaatcgcgc acacgggaaa ttcagaaaat gcgggaactt
     3901 ggcggagagc tgaaacgcca cggcatttcc ctgacaggca gcgataacac catccagcag
     3961 gccatcagac gcaccgaaca atacaacaac cagcttgaac gcgaacggca ggcgcttgcg
     4021 cgtgtaacgc gggcgcgtga gcggtattcg cgcgcgcagg aaaccgcggg aaaactgaaa
     4081 acaggtggtg cgctggcaat tggtgcggca gcggcgggcg gctatgctgc cgggcgtttt
     4141 ttgcagcccg cgatcgggtt cggcaaagag atgtcccgcg ttcaggcgct gacgcgaatc
     4201 gaccagaaca gcccgcagtt taaggcgctg cgtgagcagg cgttaaaact tggctctgaa
     4261 acgcagttca ccgcaggcga tgccgccagt gggcaggcat ttcttgcaat ggcaggcttc
     4321 acaccgcagg ccattcaggc tgcgcttccc ggagtgctga gcatggcaac ggctggcggt
     4381 atggatctcg gcgagacggc agatattggc tcaaatatcc tgacgcagtt cggcctttct
     4441 gctgaccaga tggaccgggt cggtgacaca ctcaccgcag cgtttacccg taccaacact
     4501 gaccttcgcg cactgggcga aaccatgaaa tatgcaggtc cggtggcggg taagctggga
     4561 atatcgctgg agcaggccgc agcgatggct ggtgtgctgg cgaatatggg tatcagaggg
     4621 agtgatgccg ggacggcaat gcgtgccagc ctggctcgtc tggcatcacc gccaaaggcg
     4681 gcggcagaag ctctgaaaga gctgggcgtg tccgtctcgg atgccggggg taaaatgcgc
     4741 ccgatggagg atgtgctggc cgacctttat aaagccaccc gcaaatacgg ggaagttgac
     4801 cgggtatcgt tctttaagga cattgccgga gaagaggctt tcacatcgtt tatggcgctc
     4861 gttgatgcag tgggtgacgg ttccttaccc aaactgagaa aagaacttga aggcgcgcgc
     4921 ggtgaggctg aacgcacagc aaaggttatg gccaacaacc ttgacggcga tctgaaatca
     4981 ctcggcagtg catgggaagg gttgcgcatc cgcattgcag atctgattga cggtccgctg
     5041 cgttctgtca cgcagtggct cacgcgggtg gtatcaaggg tgacggcgct ggcgcaggcc
     5101 catccggcac tgacgcgcca gctactgata gcaggcggtg cgttgctggc aatgactgca
     5161 acggttggct cgttgtcgct ggctattggt gtgcttgctg gtccgctggc aaaactgcgt
     5221 cttggttttt ctctcctgac cggatcaatg aatgctgtca gggtcctgcc agcactatgg
     5281 ggaatggtga cgggttccgt ttctttactg ggaggcgcta tcggggcgct gttcagtccg
     5341 gttggtctta tcgtggctgc gcttgccgga gctgccgtcc ttatctggaa atactgggat
     5401 cccatcaggg cattttttgc cggggtgttc agcgggatta tggaaaggct gaacccgttg
     5461 cgtgaaacct ttgaacggtt tggccctgtt tttgacgcaa tcggaagcgg gatcagccag
     5521 gtgtttaact ggtttaaatc gctgctgtca ccgatggagt ccagcaagga aacgctggat
     5581 aaatgtacca gtgctggcga gatattcggt aacgttcttg gcggtgcgtt acaacttgtt
     5641 ctgacacctg caaaaatgct actggatacg ctggcgtgga tacttgaaaa acttggcgtc
     5701 cttccggatg aagcggaaag ggcgcgcaag aaaatcgaag acgcacagcg tgcggccatt
     5761 cttcaggata aggttgcctt acttcagggg gacctggcga aaatcaatcc gccgaagcct
     5821 gtggaaaatg gcaatggcac cggaggtgat aaacccaaag acaacaaacc gctcacagac
     5881 agcaataccg ggacgctacg cagactcagc aaaattgctg ataacacagg taagctggtt
     5941 gatgagacga aaaaacgcat tggccctggc gatattgtct ttaagaacct gccccgcgca
     6001 cttgctgttc gtggggagtg gcaggagcgg aagattgcgc aggtcagtaa gcctgccccc
     6061 gcaattaata tcacacccgt agtcccggct ccgctgcctc cggcgctggt ccctgttgtt
     6121 gcggccagct cccgcccggt ggcggaggcc atacgatcgc cagtggcatc agttcctgca
     6181 gcttcccgta atcgggagcc tgctgtctcc agatttggcg gtgaaattca tgttcatctg
     6241 cataacgttg ttacgcagaa tccccgcgaa ctggcgaaac tggtcggtga aatggtcagg
     6301 gcagaaatgg aacggcgcgc ccgtgccggg cgtggcagtt tttacgataa agattgagga
     6361 gtcatggcca tgatgatgat ctatggcatg tttgtttttg agctgcgcac gctgccgcat
     6421 cagcagttac agcaaaacaa aagctggcgg catgtgaaaa atgaacgcgt taaccgttca
     6481 gcaagctggc agtatatcgg tgcaggtgat gatcgcatcg ttctttctgg tgtgctttat
     6541 cctgaaatta caggtggcga agtgtcgctg tctctgctga ccacgcaggc gtatacagga
     6601 cggccctggc ctctgattga tggcgtcggg cagatttacg gcatgtatgt cctgaccgaa
     6661 acgaatacga cccgctccga gtttgatcgc tacggtaagg cgaaaaagat agaattttca
     6721 ctgactcttg aacgctgtga tgaggatttg cgggagcgcc tgcaatcctc atcgttcagc
     6781 gatatgttgt ccggcttcaa agataaggtg acatcatccc ttaatagcgc gaccagctca
     6841 gttaaggggc tgctctgatt taacgtatgt cgccaatttc ctgatgaagg tgactggcga
     6901 cttgctgttg tatgtccttc tcagaaaatt gtttttgaat aacaaacaac aggattttat
     6961 aatctcttaa ccttataata tgtgtggtct g
//
LOCUS       DAZFRJ010000211         6991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000211 DAZFRJ010000000
VERSION     DAZFRJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.213"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(16..2799)
                     /locus_tag="ACHKUN_004069"
     CDS             complement(16..2799)
                     /locus_tag="ACHKUN_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6662227.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2856..5228)
                     /locus_tag="ACHKUN_004070"
     CDS             complement(2856..5228)
                     /locus_tag="ACHKUN_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO6662228.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5266..6951)
                     /locus_tag="ACHKUN_004071"
     CDS             complement(5266..6951)
                     /locus_tag="ACHKUN_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO6662229.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
BASE COUNT         1806 a         1608 c         1568 g         2009 t
ORIGIN      
        1 ttgacggggc tgttattatt ttggcaataa tactccggta taagtattta ccggatgaga
       61 aagatattgt ttaacggcag tgttaacatt ctctaccgtc atttgtttca acaattgctc
      121 ctgctcagtc catgctgcag gatcgtcata ttgaataaga ctatttacaa tagtgttcgc
      181 taattgttga acgctacgct gttggatatc gaggctgcgc tgaacgtttt gctggtattc
      241 attcagttct tgctcactga tccctttagc cagacgctta accatcactt cattcgctaa
      301 cgttaacagt tcatcatgtc gttctggttg acaagtaaaa gccagcaaat gactgatatc
      361 tttggcctga ggatcaaccg agaggcgaga agaaacgctg tatgctccag atgcctgttc
      421 acgaatatta acacgtagat cttttgccag tgcgacgtta aaagcatcga gcgccatacg
      481 cgtcgccaga ttaacaggtg tccgggaatc ataacgcttc cactgtgaaa cctgtgccac
      541 aggttcattt tgttctttta cagtaaccga tgcgttgtcc gtcgcgcgag ttaatggttt
      601 acctgcggct aatggcgaat cagagtgttt gattgatcct aagtaacgcg taattaacgc
      661 cacgagtttg tcttctgaga cattaccgac aatgacaaac gtgatatccg ctggagatga
      721 aaacaactgg cgatcggcag ccagcgcatc tgcggcagta aactgtacaa tctgattttc
      781 ttgcagtaat ttcgtgcggt catcagcata gcgcgtctca tacatctgct gggcgaattt
      841 ctccgctgga cgctggtcga gcgttttcaa cgcctgaatt tgagcatttt gtagcgatgc
      901 ccaaatattg tcgttaatcg tgctgtgggt gattcgctgg ttaatcaact gaaaaccagg
      961 ttcagggtta ttagtccgcg cgctaacgga gagcaacgta ttcatgccac tgactttact
     1021 gctcatggtt accgaatttt ccgcactcca gcgtttcagg ctggaagagg agagctcacc
     1081 aacgccgctt ccgcttactg ctttatttgc cagcgcgata agtgattttt gctgcgcagg
     1141 gaaacttaaa tcgcctttat tcgatacggc agtaatttgt agcttttgct cttcacccgc
     1201 ggattttgcc agaataaccc tggcaccatt ggaaagtgtt aatgatgtca gattctcagc
     1261 cagcgtttct ttgctgctaa tttccgcctg tgggtcagcg tctactgtta acgataaatt
     1321 tctgcctggg aagatgtaag ccgccagctt tttgttggcg tactcttttt ccagcgccag
     1381 gatagccgca ggagacaatg cttttttggc ggcaagctca ttgtttacca tttgctccca
     1441 aaatgcgtcc tggttctttc ttaactgctg ccatttttcc gccagacttt gcacggtaat
     1501 ttgctgccac agacgtttcg aaagttgata tgtctcttcc ggcgacaaga acggcgtatt
     1561 atttaatgag ctggatgcca ggcgactggt cagcatacgt aaatcacgtt cagcttgctg
     1621 atcaaccgca tttttcagcc aggtgaggcg ggtagattta acatcatcga gttcttcagc
     1681 agaaaaacca tgctgatcaa tagttgccaa ctctgccatt aatgcattcg cagcatcctg
     1741 catattatcg tctcgtgcat ttacacggaa aaacagcgac tgataatcgg gtgcaatttt
     1801 aacgctgcgc gcagtgccgc cagaaatagt cttcaactcg cccgactgta tgcgttcctg
     1861 cagacgttga ttgaacagct gaactaacat gctccattca gcttgttcga taaagctttg
     1921 ctcatcgtgc acttgtacca ttggcaggcg ataatagagt gcgatgccgt tcacccggtt
     1981 ttctttatca ttaatgatat taaagcgcag gtggttttcg gcttttgtcg gccagacgcg
     2041 attttcagct gctttgttag ccggaagctt acttaaatta tcctttatca gcgccagcgc
     2101 ttctttactg tcgatatcgc caaccacgat aaaggtcata ttatttggtt gataccagcg
     2161 ttgataaaat tggcgcaatt gtgccggtgt gaccgtggcg acagtatcca tcaggccgat
     2221 aggttcacgg tctaaattac gggtattagc cagcaggaaa gggcggcgcg cctgagaggt
     2281 gcgccatttc gcatcctgat gggcacgcca ttcctcagta attacgccac gttcagcgtc
     2341 tacttcgagt ttttcaaagg ttgcggcatt actccattca ctgaagattg ccatcacttg
     2401 ttgcagattt tgtttctgcg tagtcggcaa actcacctga tacaccgttt cgtcatagct
     2461 ggtataggca ttaacatcgc gaccaaaacg caggcccatt gactcaaatg tttcgatgac
     2521 tttattaccc ggccatgttt ttgtgccgtt aaacatcata tgttctacaa aatgagccac
     2581 gccgcgctca ttgtcttctt cctgcaatga accggtatga atttgcagcc ataaatttac
     2641 ctgatctttt ggatgagcat gcggataaat catatatcgc aagccattgc ccagttgccc
     2701 ggtaattaac ttttcatcct gcggtaaggc ggcggcaatc agccgcccgg ggagcaacag
     2761 agttgccact aacgtcagta agaaacagag gtttctcata attatctcca tgcgaaaacc
     2821 gggcgaattt acccggttaa gtaaaatccg aactattaaa atttcatgct gacatcaagc
     2881 cagaaagtac ggccactggc gtaggtcgcc acaccggtat tcgttgtatc aacagcggtt
     2941 ttgctatcca atacattgag aatatcggca ctgatcgtca ggttttgttg tttcagaaat
     3001 tgtggcgtcc aggacaagcg ggtgtcccag gtcaggctgc tatccagttt ttcgtcaaca
     3061 tactgcttgt aatctgaata ttcgctgatg tattgcgcat ttgtcttacc caggataatg
     3121 cgagctttac gcgcttcttg ccaggccagc gtattcgccc acactaaccc gctcggttga
     3181 tgcgtgaaat ccatgtttaa ggagatcttt aatgggttat taaaatctgc cactggaacg
     3241 ctatcgtaag aaaccagatt accgttataa accacctggt tatcaccggt attgctctcc
     3301 tcataaccat tgtttaacga caagttgccc ttgctcttga tatagctaaa gacaatttgc
     3361 gggttaatat ctacctggct gatatgcagg ggttcggcca gttcaaaact gaggttgaac
     3421 gaatgcgttt ttgttttgcc gtcgttgtta tattcagtaa tggtggtttt agtcgcgctg
     3481 tcggtacgac tgcttttgct gatttgatca tgcgcttcac ggtaaacata gtttgcgcgc
     3541 gcaataacgt tcttaccgat tttctgctgc aatcccattg ccagttcatc gttataaggc
     3601 gttttcaaat cctgataacg cgtcagggtt ttattgcctg ataccgattc cgtccagcta
     3661 ttgcggatat cacgtaatcc catatcaaga atattcccgc catagtaacg gttataacct
     3721 gctgtaatca ttgaggtttg atcagcaaaa atatcccatt ccgtcataaa gcgcggggag
     3781 atattgtggt ttgacagata gttgtcatag tcataacgca caccgggcat taacgacaca
     3841 ttccgccagc taatgtgatc cgccatatac agcgtgtagt tgtcaattcc caggctgcct
     3901 ttacctttat ggtaaatggt atggttagtt ttctttcctg cagcattaat cacataggat
     3961 tcagactggt tatggcgttc agtccatgca tcggaataga tgtattccgc gccgaagtag
     4021 ggttgatgcg aaacatcacc cacggcgaat ttttgccagt ccaggcgtgt tttgaaggtg
     4081 taattatcta cagcctggga aatgtgtcct aatccgccac gggtacaacg ccctgtaata
     4141 tcaccatatg tacatgaaag ttcggtgtac cagatgtcat gatcgtgacg ggtataatca
     4201 ctaatatgat cccaaccaac ggtggtacgc agtttggccc atgcgagctg cgtatccata
     4261 tcccatgcca gaccgtagga tttattaccc atttcgcgat cagactgcgg gaaggtgctg
     4321 gtattataat cacggctgga gccggtatat tttaaggtta aatcgtgggt aaagcggtcg
     4381 ctggcaaacc aggtaaattt gctcaatgca gtatcgataa cgtttttata ctgtgcccga
     4441 ccggcgacaa tgccgtcatt cgaaacataa tccgcgcggg tgatatcaga ctggcggcgc
     4501 gataaaccgg cggtaacgcc gaagttatca gcgagttcct gattaaacga caaggtataa
     4561 aagttctttt taaaatctgg ggagtaataa gtacttcctg aagagccttg attaaatgcg
     4621 ctcttgttat tctcatcgat atgcgatgtt aaccagtccg aacgcgtagt gcgataaccc
     4681 aatttcacct tgctatcatc agcgttgaag cgtttgatct ttgcatcaat taccccgcca
     4741 ttgaagcgac caaattcaac cggcacaaca ctgtcataaa gcgtcacatt gtccagtaag
     4801 ctgacatcaa gataataccc ctgtgacatc ccgctaatat tggttgcact actggcatcg
     4861 gactcattcg ctgggttcaa gttattagtg gcactaatac cgtcaatcaa ataggcattc
     4921 tgatagggcg acgcaccgtg aatagagatt ttctccgggc gaatatctcc ctggttcaac
     4981 gaggtacttt gcgttgaatc catgcgtacc gcagggttgg ttctcagcag atcgctgata
     5041 ttgccattcc cggtcggtaa acgctcgatg ctttcactgg tgtaatgcgt gttgccgttt
     5101 acgggtacag ggacaggagc atagaccgtc atctcttcaa aaacatgcat gtacatgttt
     5161 ttgtcatcga cggcctgcgc cacatcagcg ccacataaaa tgacgccagg aataagaact
     5221 cgcttcattt atttatatct ccgtacagca gggcttttat tgtttttata aaaccgcgct
     5281 aatgtcacaa atatcatctg ccaggttcca gacaccggat tgatgtgtaa ccataatgac
     5341 gccgcttgtg ggtagttttt cacgcactaa acgcagtaag cggatagcct cttgttcctc
     5401 aagatgagag gtagtttcgt caagaaatat ccatttcgga cgtcgtaaaa ttaatcgtgc
     5461 cagggcgata cgttgttttt cgccgctgga aagaatatct ccccagcgat cgtggtcatg
     5521 aatacgcgca gccaatttcc ccagaccaac ctgatgcagt acttcgctca acgatttatc
     5581 gtctacgggc aggggaagtg ctttacaaat aatctctttc agtaagccgg ttttgattaa
     5641 cggtgtttgt gacacatacc aactgtcagc aggagaagaa atatcacctt taaaccacgg
     5701 ccagcagtga gataatgttt taagcagtgt ggtttttccc gcaccagagt aacctttcag
     5761 taatagccat ttgcctggcg aaacatgaaa gttcaggttc tctaatatga tcttattatc
     5821 aggagtacga atactcgcgt cagccacttg taccgcatgt tggcaatttt taggtttatt
     5881 cgtagggcgc tgttcggtga gttgatggaa ctcatacaaa cgatcgataa ccgcagccag
     5941 ttcagcaagt tcgtcatatt tataaataaa ccagcttaaa ttgttcgata ccagcataaa
     6001 tgcctggcgc gatttcatca gtccgcccag attgatctgc ccgctaataa actgcggcaa
     6061 taacagaaag tagggaagaa cgctaagcga gcgcgaataa atattctgcc agtaatcaag
     6121 ccaccgctga cgattcatta aacgatgcca gttctcttta atggtatgaa aattgtcgct
     6181 caactcctgg cgttgtagag actcagcatt gcttagcgca atcagttctg cctgcttatt
     6241 atgctgcaca agatttgttc gaaatgtcgc ttcgctacgt tgtttttcca cattaagcgg
     6301 acgaatacgt ttaccaacct tatgggtaaa taaagttcca ccgatcacaa tgagcacaac
     6361 ggtatagacc atatacccct ggatattcca ttctgttcca ccaacagtga atgagagcgt
     6421 acccgcgctt tgccaaagaa taacggtaaa ggtgatcagc atgctaagtg actggatgaa
     6481 gccaaatgaa aggctgagcg ttttgctgat cagtaagaga atgtcttcag cgatacgttg
     6541 gtcagggtta tctgtatttt tatgctcgcc gtagatctgc gtgaagtagt agtttttatc
     6601 tgcaaaccac cgattaaggt agtaatctgt taaccattcg cgccagcgaa tagtcagtag
     6661 tttaattaac catgttttgt ttacggatat taatacaaag atccccagca gggcagggaa
     6721 ccaaaggaca agctgccaaa gcttatcggt ttctttctgg cttagcgcat tgaaaaaatc
     6781 attattccag tcgtttaacc agacctgaat tttaaccacg ccaaggatca tggcgaggat
     6841 aataatgatt aacaacactg aagttttatt attcttacgc agccaaaagg gcttcagcag
     6901 acagagatac tttgctatca acatacaaaa cgtaatggga atggttatca ttagcgaaaa
     6961 ttgattgtgc catccaatga ttatctatgg c
//
LOCUS       DAZFRJ010000212         6990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000212 DAZFRJ010000000
VERSION     DAZFRJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.214"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            436..1425
                     /gene="araF"
                     /locus_tag="ACHKUN_004072"
     CDS             436..1425
                     /gene="araF"
                     /locus_tag="ACHKUN_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO6662230.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1495..3009
                     /gene="araG"
                     /locus_tag="ACHKUN_004073"
     CDS             1495..3009
                     /gene="araG"
                     /locus_tag="ACHKUN_004073"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO6662231.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3024..4010
                     /gene="araH"
                     /locus_tag="ACHKUN_004074"
     CDS             3024..4010
                     /gene="araH"
                     /locus_tag="ACHKUN_004074"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO6662232.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4177..4977
                     /gene="otsB"
                     /locus_tag="ACHKUN_004075"
     CDS             4177..4977
                     /gene="otsB"
                     /locus_tag="ACHKUN_004075"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO6662233.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4952..6376
                     /gene="otsA"
                     /locus_tag="ACHKUN_004076"
     CDS             4952..6376
                     /gene="otsA"
                     /locus_tag="ACHKUN_004076"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO6662234.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6383..6811)
                     /gene="uspC"
                     /locus_tag="ACHKUN_004077"
     CDS             complement(6383..6811)
                     /gene="uspC"
                     /locus_tag="ACHKUN_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO6662235.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1775 a         1632 c         1814 g         1769 t
ORIGIN      
        1 gagtacagtg ataattaatg gcgaaatgaa tgacgtgcat tttccacatc tttgagttgc
       61 ggttattaca ccatttcaaa aactcaacgc caggtaatgc ggcctattga ctggttaaaa
      121 agaagacatc ccgcatgggt accaaagaca acaaggattt ccaggctaat cttatggatt
      181 aatctgctgt gcattcgaca atttgtctga caaattggct ttcccttatg tcttttcccg
      241 ctaaatttat gcacgttctc actgtaattc tgcgatgtga tattgctctc ctatggagaa
      301 ttaatttctc gctaaaacta tgtcaacaca gtcacttatc ttttagttaa aaggtaatgc
      361 tttgttttcc gattaattta acgaatgtca ttcgtttttg ccctacacaa aacgacacta
      421 aagctggaga gaaccatgca caaatttact aaagccctgg cagccattgg tctggcagcc
      481 gttatgtcac aatccgctat ggcggagaac ctgaagctcg gttttctggt gaagcaaccg
      541 gaagagccgt ggttccagac cgaatggaag tttgccgata aagccgggaa ggatttaggg
      601 tttgaggtta ttaagattgc cgtgccggat ggcgaaaaaa cattgaacgc gatcgacagc
      661 ctggctgcca gtggcgcaaa aggtttcgtt atttgtactc cggaccccaa actcggctct
      721 gccatcgtcg cgaaagcgcg tggctacgat atgaaagtca ttgccgtgga tgaccagttt
      781 gttaacgcca aaggtaagcc aatggatacc gttccgctgg tgatgatggc ggcgactaaa
      841 attggcgaac gtcagggcca ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc
      901 aaagaaagcg cggtgatggc gattaccgcc aacgaactgg ataccgcccg ccgccgtact
      961 acgggatcta tggatgcgct gaaagcggcc ggattcccgg aaaaacaaat ttatcaggta
     1021 cctaccaaat ctaacgacat cccgggggca tttgacgctg ccaactcaat gctggttcaa
     1081 catccggaag ttaaacattg gctgatcgtc ggtatgaacg acagcaccgt gctgggcggc
     1141 gtacgcgcga cggaaggtca gggctttaaa gcggccgata tcatcggcat tggcattaac
     1201 ggtgtggatg cggtgagcga actgtctaaa gcacaggcaa ccggcttcta cggttccctg
     1261 ctgccaagcc cggacgtaca tggctataaa tccagcgaaa tgctttacaa ctgggtagca
     1321 aaagacgttg aaccgccaaa atttaccgaa gttaccgacg tggtactgat cacgcgtgac
     1381 aactttaaag aagaactgga gaaaaaaggt ttaggcggta agtaatttgc cggaaaaatt
     1441 cccctctgca tgatgcagag ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa
     1501 cagtctaccc cgtatctctc atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg
     1561 ctgacggata ttagttttga ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat
     1621 ggcgcaggaa aatcaactct cttaaaaatc ctcagcggca actatgcgcc aaccacgggt
     1681 tctgtagtga ttaatgggca ggaaatgtcc ttttccgaca cgaccgcagc acttaacgcg
     1741 ggtgtggcga ttatttacca ggaactacat ctcgtgccgg aaatgaccgt cgcggaaaac
     1801 atctatctcg gccagctgcc gcataaaggc ggcattgtga atcgctcatt gctgaattat
     1861 gaggcgggtt tacaacttaa acatcttggt atggatattg acccggacac gccgctgaaa
     1921 tatctctcca ttggtcagtg gcagatggtt gaaatcgcca aggcgctggc gcgtaacgct
     1981 aaaattatcg cctttgatga gccaaccagc tccctttctg cccgcgaaat cgacaatctt
     2041 ttccgcgtta ttcgtgaact gcgaaaagag gggcgggtga tcttatacgt ttctcaccgt
     2101 atggaagaaa tatttgccct cagcgatgcc atcactgtct ttaaagatgg acgttatgtc
     2161 aaaaccttta ccgatatgca gcaggttgac cacgacgcgc tggtgcaggc gatggtcggg
     2221 cgcgacattg gcgatatcta cggctggcaa cctcgtagtt atggcgagga gcgcctgcgt
     2281 cttgatgctg tgaaagcacc aggcgtgcgt acgccaataa gtctggcggt tcgcagtggt
     2341 gaaattgttg ggctgtttgg tctggtaggg gcggggcgta gcgaattaat gaaaggcttg
     2401 tttggcggga cgcaaatcac cgccggtcag gtttatatcg accaacagcc gatcgatatt
     2461 cgtaaaccga gccacgccat tgccgcaggc atgatgctct gcccggaaga tcgcaaagcg
     2521 gaaggcatta ttcccgtgca ctccgttcgc gacaatatca acatcagtgc cagacgtaaa
     2581 catgtgctcg gcggttgtgt aatcaacaac ggttgggaag aaaacaatgc cgatcaccac
     2641 attcgttcgc tcaacatcaa aacgccgggc gctgagcaac tgatcatgaa tctctcaggc
     2701 ggaaatcagc aaaaagccat tctgggccgc tggttatcgg aagagatgaa ggtcattttg
     2761 ctggatgaac ctacgcgcgg cattgatgtt ggtgctaagc atgaaattta caacgtgatt
     2821 tatgcgctgg cggcgcaggg tgtggcggtg ctgtttgcct ccagcgactt acctgaagtc
     2881 ctcggcgttg ccgaccggat tgtggtgatg cgggaaggtg aaatcgccgg tgaattgtta
     2941 cacgagcagg cagatgagcg tcaggcactg agccttgcga tgcctaaagt cagccaggca
     3001 gttgcctgag taaggagagt atgatgtctt ctgtttctac atcggggtct ggcgcaccta
     3061 agtcgtcatt cagcttcggg cgtatctggg atcagtacgg catgctggtg gtgtttgcgg
     3121 tgctctttat cgcctgtgcc atttttgtcc caaattttgc caccttcatt aatatgaaag
     3181 ggttgggcct ggcaatttcc atgtcgggga tggtggcgtg tggcatgttg ttctgccttg
     3241 cttccggtga ctttgacctt tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg
     3301 cggtggttat caacctgact gaaagcctgt ggattggcgt ggcagcgggg ttgctgcttg
     3361 gcattctctg tggcctggtc aatggctttg ttatcgccaa actgaaaata aatgctctga
     3421 tcacaacact ggcaacgatg cagattgttc gaggtctggc gtacatcatt tcagacggta
     3481 aagcggtcgg tatcgaagat gaaagcttct ttgcccttgg ttacgctaac tggttcggtc
     3541 tgcctgcgcc aatctggctc accgtcgcgt gtctgattat ctttggtttg ttgctgaata
     3601 aaaccacctt tggtcgtaac accctggcga ttggcgggaa cgaagaggct gcgcgtctgg
     3661 cgggtgtacc ggttgttcgc accaaaatta ttatctttgt tctctctggc ctggtatctg
     3721 cgatagccgg aattattctg gcttcacgta tgactagtgg gcagccaatg acgtcgattg
     3781 gttatgagct tattgttatc tccgcctgcg ttttaggtgg cgtttctctg aaaggtggca
     3841 tcggaaaaat ctcatatgtg gtggcgggta tcttaatttt aggcaccgtg gaaaacgcca
     3901 tgaacctgct taatatttct cctttcgcgc agtacgtggt tcgcggctta atcctgctgg
     3961 cagcggtgat cttcgaccgt tacaagcaaa aagcgaaacg cactgtctga tgcttttttc
     4021 cacaacaatt taacgttttt tcccaccaca gccagccgcc acaacggttg gctgttcttc
     4081 attgcaaatg gcgacccccg tcacactgtc tatacttaca tgtctgtaaa gcgcgttctg
     4141 cgcaaaacaa taagaaaaga gaaggaggag agccgggtga cagaaccgtt aaccgaaacc
     4201 cctgaactat ccgcgaaata tgcctggttt tttgatcttg atggaacgct ggcggaaatc
     4261 aaaccgcatc ccgatcaggt cgtcgtgcct gacaatattc tgcaaggact acagctactg
     4321 gcaaccgcaa gtgatggtgc attggcattg atatcagggc gctcaatggt ggagcttgac
     4381 gcactggcaa aaccttatcg cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac
     4441 atcaatggta aaacacatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg
     4501 caactgcata cagtcatcgc tcagtatccc ggcgcggagc tggaggcgaa agggatggct
     4561 tttgcgctgc attatcgtca ggctccgcag catgaagacg cattaatgac attagcgcaa
     4621 cgtattactc agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc
     4681 aaaccgagag gtaccagtaa aggtgaggca attgcagctt ttatgcagga agctcccttt
     4741 atcgggcgaa cgcccgtatt tctgggcgat gatttaaccg atgaatctgg cttcgcagtc
     4801 gttaaccgac tgggcggaat gtcagtaaaa attggcacag gtgcaactca ggcatcatgg
     4861 cgactggcgg gtgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa
     4921 caaaaaagag aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg
     4981 gattgcacca ccagacgagc acgccgccag tgccggtggc cttgccgttg gcatactggg
     5041 ggcactgaaa gccgcaggcg gactgtggtt tggctggagt ggtgaaacag ggaatgagga
     5101 tcagccgcta aaaaaggtga aaaaaggtaa cattacgtgg gcctctttta acctcagcga
     5161 acaggacctt gacgaatact acaaccaatt ctccaatgcc gttctctggc ccgcttttca
     5221 ttatcggctc gatctggtgc aatttcagcg tcctgcctgg gacggctatc tacgcgtaaa
     5281 tgcgttgctg gcagataaat tactgccgct gttgcaagac gatgacatta tctggatcca
     5341 cgattatcac ctgttgccat ttgcgcatga attacgcaaa cggggagtga ataatcgcat
     5401 tggtttcttt ctgcatattc ctttcccgac accggaaatc ttcaacgcgc tgccgacata
     5461 tgacaccttg cttgaacagc tttgtgatta tgatttgctg ggtttccaga cagaaaacga
     5521 tcgtctggcg ttcctggatt gtctttctaa cctgacccgc gtcacgacac gtagcgcaaa
     5581 aagccataca gcctggggca aagcatttcg aacagaagtc tacccgatcg gcattgaacc
     5641 gaaagaaata gccaaacagg ctgccgggcc actgccgcca aaactggcgc aacttaaagc
     5701 ggaactgaaa aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt
     5761 gccagagcgt tttctcgcct atgaagcgtt gctggaaaaa tatccgcagc atcatggtaa
     5821 aattcgttat acccagattg caccaacgtc gcgtggtgat gtgcaagcct atcaggatat
     5881 tcgtcatcag ctcgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg
     5941 gacgccgctt tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg
     6001 ctactctgac gtgggcttag tgacgccact gcgtgacggg atgaacctgg tagcaaaaga
     6061 gtatgttgct gctcaggacc cagccaaccc gggcgttctt gttctttcgc aatttgcggg
     6121 agcggcaaac gagttaacgt cggcgttaat tgttaacccc tacgatcgtg acgaagttgc
     6181 agctgcgctg gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga
     6241 aatgctggac gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga
     6301 cctaaagcag atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt
     6361 tccaaagctt gcgtaggagc ggttaatctc ccgtaagtgg aactaacagc acatcgacct
     6421 cacttgaggc aataactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt
     6481 gattaccgca aatcaccaaa tcgaaatgat gtttgtgaca tacttccaga atatgttcgc
     6541 ttaactcacc gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact
     6601 tatcaagaaa gctttgcgtt tcttcttgca tcacgctgcg taaatcttca agcatcggcg
     6661 cggctaattg attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct
     6721 ttactggcct ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg
     6781 caacagcgac aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg
     6841 tgacattaaa gctttactcc taatggagaa acgacgcaat cccaactcgg tcaaacaggt
     6901 aaatattagt ctgattatta gtaaagacaa ttaatcaatt actcccggtg atcatataat
     6961 ttcaaatgta attataagaa aatagtgtac
//
LOCUS       DAZFRJ010000213         6965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000213 DAZFRJ010000000
VERSION     DAZFRJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.215"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..1226
                     /gene="citC"
                     /locus_tag="ACHKUN_004078"
     CDS             168..1226
                     /gene="citC"
                     /locus_tag="ACHKUN_004078"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO6662236.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1241..1537
                     /gene="citD"
                     /locus_tag="ACHKUN_004079"
     CDS             1241..1537
                     /gene="citD"
                     /locus_tag="ACHKUN_004079"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO6662237.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1534..2442
                     /gene="citE"
                     /locus_tag="ACHKUN_004080"
     CDS             1534..2442
                     /gene="citE"
                     /locus_tag="ACHKUN_004080"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO6662238.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2453..3985
                     /gene="citF"
                     /locus_tag="ACHKUN_004081"
     CDS             2453..3985
                     /gene="citF"
                     /locus_tag="ACHKUN_004081"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO6662239.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLATFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3989..4540
                     /gene="citX"
                     /locus_tag="ACHKUN_004082"
     CDS             3989..4540
                     /gene="citX"
                     /locus_tag="ACHKUN_004082"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO6662240.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4515..5393
                     /gene="citG"
                     /locus_tag="ACHKUN_004083"
     CDS             4515..5393
                     /gene="citG"
                     /locus_tag="ACHKUN_004083"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO6662241.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5444..6907
                     /gene="citT"
                     /locus_tag="ACHKUN_004084"
     CDS             5444..6907
                     /gene="citT"
                     /locus_tag="ACHKUN_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO6662242.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
BASE COUNT         1559 a         1905 c         1847 g         1654 t
ORIGIN      
        1 aaatttcacg ccgaaatact ccttaggatg tatagcgaaa ggagaaaaag atatacctcg
       61 atcacccccc tttctcccaa gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca
      121 ttcgttgcaa atcgataaca acatgcacct tcaggatact atttattatg ttcggcaatg
      181 atattttcac ccgcgtaaaa cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc
      241 tgcatgaaaa tgatttgagc gttgacacca cagtcgaagt atttattacc gtaacccgcg
      301 atgaaaagct tatcgcgtgc ggtggaattg ccggaaatat tattaaatgc gttgctatca
      361 gtgaatccgt tcgcggtgaa ggactggcgc tgacattagc cactgaactg ataaacctcg
      421 cctatgagcg gcacagcacg catctgttta tttataccaa aaccgaatac gaggcgctgt
      481 tccgccagtg cggtttttcc acgctgacca gcgtacccgg cgtgatggtg ctgatggaaa
      541 acagcgccac gcggctgaaa cgctatgccg aatcgctgaa aaaatttcgt catccaggga
      601 acaagattgg ctgcattgtg atgaacgcca atcccttcac gaatggtcac cgttatctga
      661 ttcaacaagc tgcggcacag tgcgactggt tgcatctgtt tttagtcaaa gaagattctt
      721 cacgcttccc ctatgaagac cggttggatc tggtgttaaa aggcaccgcc gatattccac
      781 gcctgactgt gcatcgcggc tccgaataca tcatctcccg cgctacgttc ccttgctact
      841 tcattaaaga acagagcgtc attaaccatt gttacaccga aattgatctg aagattttcc
      901 gtcagtacct cgctcccgcg ctgggtgtaa ctcaccgctt tgtcggtact gaaccctttt
      961 gtcgcgttac cgcccagtac aaccaggata tgcgctactg gctggaaacg ccgactatct
     1021 ccgcaccgcc catcgaactg gttgaaattg agcggctgcg ttaccaggag atgccgatat
     1081 ccgcttcccg ggtacgtcaa ctgctggcga aaaacgatct cacggctatc gcgccgctgg
     1141 tccctgcagt cacgctgcat tatttgcaga acctgcttga gcactcccgc caggacgcgg
     1201 cagctcgtca aaagaccccc gcatgagaaa caggtgaaaa atgaaaataa accagcccgc
     1261 cgttgcaggc acccttgagt ctggggatgt gatgatacgc atcgccccac tcgatacgca
     1321 ggatatcgac ctgcaaatca atagcagcgt tgagaaacag tttggcgatg caattcgcac
     1381 caccattctg gacgttctcg cccgctacaa cgtgcgcggc gtacagctga atgtcgatga
     1441 caaaggcgca ctggactgca ttttacgtgc acgactggaa gccctgctgg cacgcgccag
     1501 cggtatcccg gctctgccat gggaggattg ccaatgattt ccgcttcgct gcaacaacgt
     1561 aaaactcgca cccgccgcag catgttgttt gtgcctggtg ccaatgccgc gatggtcagc
     1621 aactccttca tctacccggc tgatgccctg atgtttgacc tcgaagactc cgtggcatta
     1681 cgtgaaaaag acaccgcccg ccgcatggtt tatcacgcgc tgcaacatcc gctgtatcgc
     1741 gatattgaaa ccattgtgcg tgtcaacgcg ctggattccg aatggggtgt taacgacctg
     1801 gaagccgtcg ttcgcggtgg tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag
     1861 gatgttctgg atattgaaaa agagatcctg cgtatcgaaa aagcctgtgg tcgtgaaccc
     1921 ggcagcaccg gcctgctggc ggcgattgaa tctccgctgg gcattacccg cgcagtggaa
     1981 atcgctcacg cttccgagcg tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc
     2041 aacctgcgta cagaacgctc cccggaagga actgaactgc tgttcgcacg ctgttccatt
     2101 ttgcaggccg cgcgctctgc gggtattcag gcgttcgata ccgtctattc cgacgctaac
     2161 aacgaagccg gattcctgca agaagccgcc cacatcaaac agctgggctt tgacggcaaa
     2221 tcgctgatca acccgcgtca gattgatctg ttgcacaacc tctacgcacc aacccagaaa
     2281 gaagtggatc acgcccgccg cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc
     2341 ggcgtggttt ccctgaacgg caagatggtg gacggtccgg ttatcgatcg cgcccgtctg
     2401 gtgctctctc gtgcagaact ttccggcatc cgcgaagaat aaggcaatca aaatgacgca
     2461 gaaaattgaa caatctcaac gacaagaacg ggtagcggcc tggaatcgtc gcgctgaatg
     2521 cgatcttgcc actttccaga actcgccaaa gcaaacctac caggctgaaa aagcgcgcga
     2581 tcgcaaactg tgcgccaacc tggaagaagc gattcgtcgc tctggtttac aggacggcat
     2641 gacggtttcc ttccatcacg ctttccgtgg cggtgacctg accgtcaata tggtgatgga
     2701 cgtcatcgcg aagatgggct ttaaaaacct gaccctggcg tccagctccc tgagtgattg
     2761 ccatgcgccg ttggtagaac acattcgcca gggcgtggtt acccgcattt atacctccgg
     2821 cctgcgcggt ccactggcgg aagagatctc ccgtggtctg ctggcagaac cggtgcaaat
     2881 ccactctcac ggcggtcgcg tgcatctggt acagagcggc gaactgaata tcgacgtggc
     2941 tttcctcggc gtcccgtcct gcgatgaatt cggtaatgcc aacggctaca ccggtaaagc
     3001 ctgctgcggc tccctcggct atgcaatggt tgatgccgac aacgcaaagc aggtcgtgat
     3061 gcttaccgaa gaactgctgc cttatccgca taatccggca agcattgagc aagatcaggt
     3121 tgatttgatc gtcaaagttg accgcgttgg cgatgctgca aaaatcggcg ctggcgcgac
     3181 ccgtatgacc actaacccgc gcgaactgct tattgcccgt agcgctgcgg atgtgattgt
     3241 caactcaggc tacttcaaag aaggtttctc catgcaaacc ggcaccggcg gcgcatcgct
     3301 ggcggtaacc cgtttcctgg aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc
     3361 ccttggcggt attaccgcga cgatggttga cctgcacgaa aaaggtctga tccgcaaact
     3421 gctggatgtg cagagctttg acagccatgc tgcgcaatcg ctggcccgta accccaatca
     3481 catcgaaatc agcgccaacc agtacgctaa ctggggttcg aaaggcgcat cggttgatcg
     3541 tctcgacgtg gtggtactga gcgcgctgga aattgacacc cagttcaacg ttaacgtgct
     3601 gaccggctct gacggcgtac tgcgtggtgc ttccggtggt cactgcgata ccgcgattgc
     3661 ctctgcgctt tccatcatcg tcgcgccgct ggtacgcggt cgtattccga ctctggtgga
     3721 taacgtgctg acctgcatca ccccgggctc cagtgtcgat attctggtca cagaccacgg
     3781 tatcgcagtt aacccggcac gtccggaact ggcagaacgt ctgcaggaag cgggcattaa
     3841 agtggtttcc attgagtggc tgcgcgaacg tgcgcgtctg ctgaccggtg aaccacagcc
     3901 gattgaattc accgaccgcg tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga
     3961 tgttgtgcat caggtgaagg aataagccat gcacctgctt cctgaactcg ccagccacca
     4021 tgcggtgtca attcccgagc tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgt
     4081 ctggctcaag cgccatcctg ttccactggt ctcctttacc gtggttgcgc ctgggccgat
     4141 taaagacagc gaggtcacac gccgaatttt taatcatggc gtgacagcct tgcgtgcctt
     4201 agccgcaaaa cagggctggc aaattcagga gcaggctgca ctggtttccg ccagcgggcc
     4261 ggagggcatg ttgagcattg ccgccccggc tcgcgacctc aagctcgcca ccattgagct
     4321 tgaacatagt catcctctcg ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga
     4381 aattctctcc cgccgcgact attcactgcc gcctcgccgc tgcctgttgt gcgagcaaag
     4441 cgcagccgtc tgcgcgcgtg gaaaaaccca tcaactgacc gatttactca accgcatgga
     4501 ggcactgctg aacgatgtcg atgcctgcaa cgtcaactaa aaccacaaag cttgcgacgt
     4561 cattaatcga tgagtacgcc ctgctgggct ggcgcgccat gctgactgaa gtcaatctgt
     4621 caccgaaacc aggcctcgtg gatcgcatta actgcggtgc gcacaaagat atggcgctgg
     4681 aagatttcca ccgcagcgcg ctggcgattc agggctggtt accccgtttc attgaatttg
     4741 gtgcctgtag tgcagaaatg gcaccggaag cggtactcca cggattacgc ccaattggta
     4801 tggcttgcga aggtgatatg ttccgcgcca ctgcgggcgt aaacacgcat aaaggcagca
     4861 ttttttcttt agggctgcta tgtgcggcaa ttggccgttt gcttcagctc aaccagccgg
     4921 taacgccaac aaccgtttgt tctacggcgg caagtttctg ccgtggtctg accgatcgcg
     4981 aactgcgtac caataattca caactgacgg caggtcaacg gttgtaccag cagcttggcc
     5041 ttaccggcgc acgcggtgaa gccgaagcgg gttatccact ggtgatcaac cacgccttgc
     5101 cgcattacct cactctgctg gatcaggggt tagatcctga actggcattg ctcgatacct
     5161 tgctcctact gatggcgatc aacggcgata ccaacgttgc ttcgcgcggt ggcgaggggg
     5221 gcctgcgctg gctacagcgc gaggcgcaaa cattattgca aaaagggggc attcgaaccc
     5281 ccgccgatct cgattatctc cggcagttcg acagggagtg tatcgaacga aatctcagtc
     5341 caggcggcag tgccgacctg ctgatcctta cctggttttt agcacagatt taattattta
     5401 agcacttgat aaatttgaaa atattaattt tcggagaacc cgtatgtctt tagcaaaaga
     5461 taatatatgg aaactattgg ccccactggt ggtgatgggt gtcatgtttc ttatccctgt
     5521 ccccgacggt atgccaccac aggcatggca ttacttcgct gtgtttgtgg caatgattgt
     5581 cggcatgatc ctcgagccaa ttccggcaac agcgatcagt tttattgcgg ttactatttg
     5641 cgttattggc agtaattacc tgctctttga tgccaaagaa ttagctgacc cagcgtttaa
     5701 tgcgcaaaaa caggcgctga aatggggtct ggctggtttt tccagcacca cggtatggct
     5761 ggtatttggc gcatttattt ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat
     5821 tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg acgttgggtt atgcgattgt
     5881 cattatcgac attctgctgg caccgtttac accgtccaac accgcgcgta ccgggggtac
     5941 ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa tcattcccca acgatccgtc
     6001 cgcgcgtcgt attggcggct atttgatgtg gatgatggtc attagtacca gtctgagttc
     6061 gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt ctggagttcg tcagcaaaat
     6121 tgccggtatc cagattagct ggttgcagtg gttcctctgc ttcctgccgg ttggggttat
     6181 cttgcttatc attgcgccgt ggctttccta cgtgctgtac aaaccggaaa tcacacacag
     6241 tgaagaagtg gcaacctggg cgggtgatga actgaaaacc atgggtgcgc tgacacgcag
     6301 agagtggaca ctgattggcc ttgtattgct cagcttaggt ttgtgggttt ttggcagcga
     6361 agtcattaat gctactgcag ttggtctgct ggcagtttcg ctaatgctgg ccctgcacgt
     6421 tgtgccttgg aaagatatta cccgctataa cagcgcatgg aacacgctgg tcaacctagc
     6481 aactctggtt gtgatggcta acggcctgac tcgttctggc tttattgact ggttcgccgg
     6541 taccatgagt acgcacctgg aaggattctc accaaacgca acggtgattg tactggttct
     6601 ggtgttctac tttgcacact acctgtttgc cagcctgtct gcgcacaccg caaccatgct
     6661 gccggttatt ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac aactgtgtat
     6721 cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg ccgtatgcaa ccggtcctgg
     6781 ggtgattatt tacggctgtg gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat
     6841 cttcggggtg atttacatct ctatgttgct gttggttggc tggccgattc tcgccatgtg
     6901 gaactaatac gttcgccaaa cgctgaataa atcatcacgc ccgccaggta agcccacctg
     6961 gcggg
//
LOCUS       DAZFRJ010000214         6955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000214 DAZFRJ010000000
VERSION     DAZFRJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.216"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..757
                     /locus_tag="ACHKUN_004085"
     CDS             71..757
                     /locus_tag="ACHKUN_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO6662243.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(848..984)
                     /locus_tag="ACHKUN_004086"
     ncRNA           complement(848..984)
                     /ncRNA_class="other"
                     /locus_tag="ACHKUN_004086"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1028..1164)
                     /locus_tag="ACHKUN_004087"
     ncRNA           complement(1028..1164)
                     /ncRNA_class="other"
                     /locus_tag="ACHKUN_004087"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1209..1296)
                     /locus_tag="ACHKUN_004088"
     tRNA            complement(1209..1296)
                     /locus_tag="ACHKUN_004088"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1260..1262),aa:Ser,seq:gga)
     gene            1530..2468
                     /gene="ghrA"
                     /locus_tag="ACHKUN_004089"
     CDS             1530..2468
                     /gene="ghrA"
                     /locus_tag="ACHKUN_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO6662244.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGADVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2523..3260
                     /gene="ycdX"
                     /locus_tag="ACHKUN_004090"
     CDS             2523..3260
                     /gene="ycdX"
                     /locus_tag="ACHKUN_004090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO6662245.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3284..3838
                     /gene="ycdY"
                     /locus_tag="ACHKUN_004091"
     CDS             3284..3838
                     /gene="ycdY"
                     /locus_tag="ACHKUN_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO6662246.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3940..4431
                     /gene="ycdZ"
                     /locus_tag="ACHKUN_004092"
     CDS             3940..4431
                     /gene="ycdZ"
                     /locus_tag="ACHKUN_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6662247.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGSFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4495..5328)
                     /gene="csgG"
                     /locus_tag="ACHKUN_004093"
     CDS             complement(4495..5328)
                     /gene="csgG"
                     /locus_tag="ACHKUN_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO6662248.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5355..5771)
                     /gene="csgF"
                     /locus_tag="ACHKUN_004094"
     CDS             complement(5355..5771)
                     /gene="csgF"
                     /locus_tag="ACHKUN_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO6662249.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5796..6185)
                     /gene="csgE"
                     /locus_tag="ACHKUN_004095"
     CDS             complement(5796..6185)
                     /gene="csgE"
                     /locus_tag="ACHKUN_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO6662250.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6190..6840)
                     /gene="csgD"
                     /locus_tag="ACHKUN_004096"
     CDS             complement(6190..6840)
                     /gene="csgD"
                     /locus_tag="ACHKUN_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO6662251.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1736 a         1610 c         1725 g         1884 t
ORIGIN      
        1 gcatgtaaca cgctgcagcg agtaacattg gtaatgaaat ataatcaaca tggataggtg
       61 aaaattaatt atgagcttta tggcttttgt aacttgtttg acaattgcat ttatcgccgg
      121 ttttgctgtt gccagaaaat tgacactccg aaaagttgcc tcatcggtaa tcatcgctaa
      181 atcgcaaccc gtcccttcat ctgcaacttc ccctgccgct aaggcgtcga caaggagcta
      241 tataccgcag aaaacctatc gtgactctcg ttggcaagag cgagatgagt tatttcaggg
      301 aatggttgcc gaaggtattt tattggaaag aaaagagtgg ttaatcacgc gttgtgaaaa
      361 aatgtatttc gataatctgg aaaaatggtt tggacattat tgtcatatcc attgccaggt
      421 ctctctgggg cgcttgatca actttccaga acaacctgag tttagcgaag aagaaagaaa
      481 gcgtttcttt gcaatttata atggcatggc aatggattat gttctggttt caaagaagaa
      541 caataaaata gtctgtgtta ttgagctgga tgacccgagt catgaaaggg cggatcgcat
      601 tattcgtgac cgttggttgg atacaattat gactcttgca cgggtgcctt ttgtacgagc
      661 ttctgtcact aaattggagg aaattcctcc ggtctgggag actcgtgaca aaattcgccc
      721 attagtcgcg gcgacaaaga gtgaaccggt gcattaacta agcatcccgg tcggggcttc
      781 tcatcccccc ggtgtgtgca atatacgaaa aaaagcccgt accttcgtac gagctcttct
      841 ttaaagatgg cggtgagggg gggattgact cgcttcgctc gccctgcggg cagcccgctc
      901 actgcgttca cggtctgtcc aactggctgt cgccagttgt cgaaccccgg tcggggcttc
      961 tcatcccccc ggtgtgtgca atatacgaaa aaaagcccgt actttcgtac gagctcttct
     1021 ttaaatatgg cggtgagggg gggattgact cgcttcgctc gccctgcggg cagcccgctc
     1081 actgcgttca cggtctgtcc aactggctgt tgccagttgt cgaaccccgg tcggggcttc
     1141 tcatcccccc ggtgtgtgaa atatacgaaa aaaaagcccg tactttcgta cgagctcttc
     1201 tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta tacacacttt
     1261 ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgtcgttcct gtcttgctgg
     1321 aacgggcgct aatttaggga aatcatgacc tgaggtcaac aaactttttg aaaaaatcgc
     1381 gcgtttattc aaacttcaat caatgtgtgg ttttaataag cgaaatctgc tttttttgcc
     1441 accgaccacg gatttgttat gctggtggcc tttgtagatc ataacgataa gtgcgaataa
     1501 atttcgcaca acgcttttcg ggagtcagta tggatatcat cttttatcac ccaacgttcg
     1561 atacccaatg gtggattgag gcactgcgca aagctattcc tcaggcaaga gtcagagcgt
     1621 ggaaaagcgg agataatgac tctgctgatt atgctttagt ctggcatcct cctgttgaaa
     1681 tgctggcagg gcgcgatctt aaagcggtgt tcgcactcgg ggccgatgtt gattctattt
     1741 tgagcaagct acaggcacac cctgaaatgc tgaacccttc tgttccactt tttcgcctgg
     1801 aagataccgg tatgggcgag caaatgcagg aatatgctgt cagtcaggtg ctgcattggt
     1861 ttcgacgttt tgacgattat cgcatccagc aaaatagttc gcattggcaa ccgctgcctg
     1921 aatatcatcg ggaagatttt accatcggca ttttgggcgc aggcgtactg ggcagtaaag
     1981 ttgctcagag tctgcaaacc tggcgctttc cgctgcgttg ctggagtcga acccgtaaat
     2041 cgtggcctgg cgtgcaaagc tttgccggac gggaagaact gtctgcattt ctgagccaat
     2101 gtcgggtatt gattaatttg ttaccgaata cccctgaaac cgtcggcatt attaatcaac
     2161 aattactcga aaaattaccg gatggcgcgt atctcctcaa cctggcgcgt ggtgttcatg
     2221 ttgtggaaga tgacctgctc gcggcgctgg atagcggcaa agttaaaggc gcaatgttgg
     2281 atgtttttaa tcgtgaaccc ttaccgcctg aaagtccgct ctggcaacat ccacgcgtga
     2341 cgataacacc acatgtcgcc gcgattaccc gtcccgctga agctgtggag tacatttctc
     2401 gcaccattgc ccagctcgaa aaaggggaga gggtctgcgg gcaagtcgac cgcgcacgcg
     2461 gctactaata aagcatcagg attcctgcta tccttggcgg gaattgaata caggagagag
     2521 ttatgtatcc cgtcgacctt catatgcata ccgttgccag cacacatgca tatagcacat
     2581 tgagtgatta cattgcccag gccaaacaaa agggcattaa actttttgcg atcaccgatc
     2641 atggcccgga tatggaagat gctccgcatc actggcactt cattaacatg cgtatctggc
     2701 cgcgagtggt tgatggggta gggatcctgc gcggcatcga agctaacatt aaaaatgttg
     2761 atggtgaaat tgattgcagc ggtaaaatgt ttgactcgct ggatctgatt attgccggtt
     2821 ttcatgagcc ggtttttgca ccacatgaca aagcgaccaa tacacaagcg atgatcgcca
     2881 ctatcgccag cggcaatgtg catataataa gccatcccgg aaatcccaaa tatgaaatag
     2941 atgtgaaagc cgttgctgaa gcagccgcga aacatcaggt ggcgctggaa atcaataatt
     3001 cttcattttt acactcacgt aagggcagtg aagacaactg tcgtgcggta gccgcagcgg
     3061 tacgtgatgc tggaggttgg gtggcattag gctcggattc tcacaccgcg tttaccatgg
     3121 gggaatttga agagtgtctt aaaatcctcg acgcggtaga ttttccgcca gagcgcattt
     3181 tgaatgtttc gccgcgccgc ttactgaact tccttgaatc tcgcggtatg gcaccgattg
     3241 cggaatttgc agacctttaa ttactagtaa tggaaatata ttaatgaacg agttttctat
     3301 cctctgtcgt gtgctgggtt cgctctatta ccgccaaccg caagatcctt tactggtgcc
     3361 gctgtttacc ctgattcgtg aggggaaact ggctgcgaac tggccactgg agcaggatga
     3421 gttactgacg cgtttacaga aaagctgtga tatggcgcaa gtctctgccg attacaatgc
     3481 gttgtttatc ggcgatgaat gtgccgtgcc gccatatcgt agcgcatggg ttgaggacgc
     3541 aacggaagcg gaagtgcgcg cttttctttc cgaacgaggg atgccactgg cggatacgcc
     3601 agccgatcac atcggcacat tgctgcttgc ggcttcctgg ctggaagatc agtcaacgga
     3661 agatgagagc gaagcactgg aaacactgtt cagtgagtat ctgttaccct ggtgtggtgc
     3721 gttcctcggc aaagtggagg cccatgcaac cacgcctttc tggcgcacca tggcaccgct
     3781 aacccgtgat gccattagtg caatgtggga cgagctggaa gaagattctg aagagtaaat
     3841 gtgatcatcg tcactcataa ctgcaacaga taatttgttg ttgcataaaa tgtgtgctcg
     3901 atctcattca tggccgcgtt ttctgctatc atgcgcggca tgaacatact tctctctatt
     3961 gcaatcacaa caggcattct ctccggtatc tggggatggg tggctgtttc cctgggctta
     4021 ctaagctggg cgggcttcct gggctgtacg gcctactttg cctgcccgca aggtgggctg
     4081 aaagggctgg cgatctccgc tgcaaccctg cttagtggcg tggtgtgggc gatggtcatt
     4141 atttacggta gtgcactggc accacatctg gaaattctcg gttatgtcat aaccggtatt
     4201 gtcgcgtttc tgatgtgtat tcaggccaaa cagctgctgc tttcatttgt tccggggtcg
     4261 tttataggcg catgcgcgac gtttgccggg cagggtgact ggaaactggt gttaccttcg
     4321 ctggcgcttg ggctggtatt tggttacgca atgaaaaaca gcggcctgtg gctggcggcg
     4381 cgtagtgcaa agacggctca ccgtgagcag gaaatcaaaa ataaagcgtg aggggcactc
     4441 acgctttcgc ttaaacagta aaatgccgga tgataattcc ggctttttta tctgtcagga
     4501 ttccggtgga accgacatat ggcggtattt caccagaatg tcgttctgcc gttctgcttt
     4561 gttctgcaaa tcccacagac cacggtcgat accatcatta atcaggaaaa tgacccctgt
     4621 ttcgatagcc gacatcagac acagcataac gggttcgttt gaggtgtaac ccacttcccc
     4681 ttccagcaag cgctggtagt caataaagcg gaaaaccccg gcctgaactt cataggaaag
     4741 tatcgtctta ctggtgttca ccgaggagag gatctcaccg gtactcacat tgacgacgcg
     4801 caggttcacg gcaatctgat cgagctggta ttgcgtgtct gcaccgatcc caaaatatct
     4861 tgccccaaca ccgcccgatt taacgttgct ttcataaccg ataatcgaac cttcaaccat
     4921 aatatttgcc gccgttaaag attgcagcgg gattcggtta ttaatggcaa ccgtgccgtt
     4981 ttcttgtgcc gcacgaataa tcttgcgctc gttaagcagg ttttgtaagc cctggcgctc
     5041 cagcggtata aaccagcgag aatctttcaa tgccgtgacc agcattgccg tggcgctttg
     5101 cggaacagca gtggagaagt tacttgccgg gtagggttta aattgcccgg tttcgtcctg
     5161 aatgttgtat accgaaacaa agatcttacc cgtcggcgct ggcagatggg tcaaatcttt
     5221 gtagctctga gctcgaggca ttaatgtcgg tctggcggct tctttaggcg gggcggttaa
     5281 gcatccgctc agtaacatga cggcaaccaa aagaaataag cgctgcatga ttattttcct
     5341 tatgaagctg gggcttaaaa atcggttgag ttattttgta aacccgaaac ctggatggtc
     5401 gaggtttgtc cggttttacg atctgtcacg ttcaactgca attgaccatc gcggttagca
     5461 atatcaacaa tataatcgtt ggtcaccatg cggcccggtt taccggtatt aatattcgac
     5521 agtagcccac ctaaaatttg tgactggatg gcctgagtaa agttatctaa cgctgagggt
     5581 gtttcaatac caaagtcatc gttatagctc ggatctttat aagagttttg ggcctgagcg
     5641 ctatttaata aaaaagcgcc attatttggg ttaccaccaa agtttggatt acggaactgg
     5701 aaagtcatgg ttccagccca acttaatggc gaaataagca tgagtagaac tactgcatgt
     5761 ttgacacgca ttgcagcctc cgaacaattt tttatttaga attcatcatg cgccaaatcg
     5821 cccgtactta ataacgcctg atttatctgg cgacgattta gtgcttcttc agtttgaatc
     5881 agtgcaaaga cgacagtttt ctcgaagtct cttttcaacg gaaataaaaa agtctggaaa
     5941 ataacgtcct gattgaccgt tatagtgatc cagcttcccc atcgtgcact gggcctttca
     6001 ttaatcgtta agttacccgt atagtcactt tcccatttat cactaaaggc tcggtaaaaa
     6061 tcatggccaa tagatgaaac agtatggtca gttaacaatc ccgggacttc tacctcaacg
     6121 gcgtgaagat tccctgcggc gaacagaaat tctgccgcca caatccagcg taaataacgt
     6181 ttcatggctt tatcgcctga ggttatcgtt tgcccaggaa accgcttgtg tccggttttt
     6241 tacggctatc ttcttgaaaa gattataaag atgcgtttta accgtatttt cgctgatgaa
     6301 caacgaacga gcgatctcgt tattagacgc gccgatacgc agcttattca ggatctcttt
     6361 ttcccgatga gtaaggaggg ctgattccgt gctgttataa cgatagttac cagaatgtgt
     6421 aatcaggtaa ctggcaagct tttgcgtaaa gtagcattcg ccgcgcagga cgccttgcaa
     6481 cccattgaca acacgttctt gatcctccat ggcataaaaa acgccgttga tatgaggcca
     6541 gttttcaatg tcgcggtacg ggtaatcttc aggcgtattt agcaacaata ttttgatatt
     6601 gttgtttttc ctgctcaaag tatcctgcca ataatggata agctttttat ctgcttccat
     6661 catatccaga agaataatag agcctgaaga tatatcgtcc agagaacgtt gaatattatg
     6721 taattttcct gtaattgcca gcgattgttt aaggtgctgc aagagagctg tcgcttgcaa
     6781 agacggttta gtgatcaaca ataatgtatg accatgaata ctatggactt cattaaacat
     6841 gatgaaaccc cgcttttttt attgatcgca cacctgacag ctgcctctaa aatagaagca
     6901 ccagaagtac tgacagatgt tgcactgctg tgtgtagtaa taaatcagcc ctaaa
//
LOCUS       DAZFRJ010000215         6852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000215 DAZFRJ010000000
VERSION     DAZFRJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.217"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..789
                     /gene="paa"
                     /locus_tag="ACHKUN_004097"
     CDS             31..789
                     /gene="paa"
                     /locus_tag="ACHKUN_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO6662252.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1125..1280
                     /gene="hokD"
                     /locus_tag="ACHKUN_004098"
     CDS             1125..1280
                     /gene="hokD"
                     /locus_tag="ACHKUN_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6662253.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1501..1758
                     /locus_tag="ACHKUN_004099"
     CDS             1501..1758
                     /locus_tag="ACHKUN_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662254.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1828..2106
                     /locus_tag="ACHKUN_004100"
     CDS             1828..2106
                     /locus_tag="ACHKUN_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662255.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2108..3163
                     /locus_tag="ACHKUN_004101"
     CDS             2108..3163
                     /locus_tag="ACHKUN_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6662256.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3164..3529
                     /locus_tag="ACHKUN_004102"
     CDS             3164..3529
                     /locus_tag="ACHKUN_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6662257.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3526..4215
                     /locus_tag="ACHKUN_004103"
     CDS             3526..4215
                     /locus_tag="ACHKUN_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6662258.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4411..4486
                     /locus_tag="ACHKUN_004104"
     tRNA            4411..4486
                     /locus_tag="ACHKUN_004104"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4444..4446,aa:Ile,seq:cat)
     gene            4577..4650
                     /locus_tag="ACHKUN_004105"
     tRNA            4577..4650
                     /locus_tag="ACHKUN_004105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4611..4613,aa:Arg,seq:tct)
     gene            4830..5258
                     /locus_tag="ACHKUN_004106"
                     /pseudo
     CDS             4830..5258
                     /locus_tag="ACHKUN_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            5255..5422
                     /locus_tag="ACHKUN_004107"
     CDS             5255..5422
                     /locus_tag="ACHKUN_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6662259.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(5617..5760)
                     /locus_tag="ACHKUN_004108"
                     /pseudo
     CDS             complement(5617..5760)
                     /locus_tag="ACHKUN_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5737..>6852
                     /locus_tag="ACHKUN_004109"
     CDS             5737..>6852
                     /locus_tag="ACHKUN_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6662260.1"
                     /translation="MSIKHYDVIRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYGTQYNTIYGAYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDR"
BASE COUNT         1747 a         1581 c         1888 g         1636 t
ORIGIN      
        1 tttcatgata accaataaaa ggagtttttt atgaggaaca taatggcagg ttttttaata
       61 ttcctgtctt ctgctgctta tgctgatatc aatctgtatg gtcctggtgg cccgcataca
      121 gccttgcttg atgcagccaa actttatgcc gaaaaaacag gtattatagt gaacgttcat
      181 tacggcccac agaacaaatg gaatgaagat gccaaaaaaa atgcagatat cttgtttggc
      241 gcatcagaac aatctgctct ggctatcatt cgggaccata aagacagctt cagtgaaaaa
      301 gatattcagc ctctttatct gcgaaaaagt attttactgg taaagaaagg taatcctaaa
      361 aatatccgga gtattgacga cctgaccaga cctgggattg gcgtaattgt taatgatggt
      421 ggtggtacca gtaatacatc aggcactggc gtctgggaag atattgccgg acgtaaaggg
      481 aatatagaaa ctgtcgccgc aatccgaaaa aatattattt tatatgcgcc caatagcgga
      541 actgcacgta aggctcttga gaatcagcct ggagcagatg tctggataac ctgggctgac
      601 tgggcagcca gtaatccaga aattggtgat gtcgtggaaa tagcgccaga ctacgtgata
      661 tggcgtgata tgaacattac agtacgtcag gatgcaaatg atgaaacccg tcgatttgca
      721 gaatggctac aaaccgatga agcggcgcct gcattcaaaa aatatggctg gaccaggaaa
      781 ggcacttgac atcctcgtcc ttcaggacgt ggattctttt tccggatgcc gcgccagcgg
      841 catgtagggg cagctcacaa aacggaaaaa attgtacgct aagcctcgcc aggtgaactg
      901 aattcattcc gatatgggaa ttcccatatc gggcgaaaac ggtttgctgt aacggcagag
      961 ttaagtagga ttgctgcggg tgcttgaggc tatctgcctc gggcatgaac accaacggca
     1021 gatagataaa agccccaccc gactataaat cgaagtgagg cccctatatg ctcgtcacat
     1081 atagattgcc tcttacggac cgaaaggtca aggagaagca ggctatgaag cagcaaaagg
     1141 cgatgttaat cgccctgatc gtcatctgtt taaccgtcat agtgacggca ctggtaacga
     1201 ggaaagacct ctgcgaggta cgaatccgaa ccggccagac ggaggtcgct gtcttcacag
     1261 cttacgaacc tgaggagtaa gagaccaggc gggggagaaa tccctcgcca cctctgatgt
     1321 gtcaggcatc ctcaacgcac ccgcacttaa cccgcttcgg cgggtttttg tttttatttt
     1381 caacgcgttt gaagttccgg acggcgccgg aatagaatca aaaatactta agtagcgcgc
     1441 agggagaaga gggatggacc ccgaacaggg gagtgctatt tatctggaag gattctgttg
     1501 atgagaatcg aagaattacg tgaaattttt agtgaagatg gcctctatac tgtgcgcgtt
     1561 gagaagggcg ctattgtcag ccactgccgt attaaatgtt tacagtctca acaaaggaag
     1621 agtggagctg cgttaattca ttttgtggat gggcttgtga cggatggttt tattttgcgt
     1681 gcaaatgaat ttgtcacatc gttgccgtct ctgaaagaag ctgggattaa ggctggtttt
     1741 tctgcttttg aagattagtg aattcatcta caattcagcg cagggctgaa cccctgttga
     1801 gtaacactgt gccaccggag aaagccgatg gcgcaaaatt ccagactaca caattctgat
     1861 aattcagccg tctttgccag caggcacggg cggcgttctc atgcattcaa atctgactgg
     1921 ttccggcacg ccccatgcac tgaagaacag gccgaatggc tgattcagaa ctaccgcaga
     1981 cgtgggtatg agtttaggaa agccctcagc ctcgattatc gtcactggat aatctacgtc
     2041 aggctgccgt actccgagcg cccaccgcgt ccgtcccgca cattccagca acgcatctgg
     2101 aggtaacgtg cgggtattac ttcgacctgt tccggtaccg gaacttgggt tggtggtcct
     2161 taaaccaggc cgtgaatcca tgcaggtatt ccataaccct cgagtgcttg tggagccgga
     2221 accgaaaagc atgcgcggtc tgccgtccgg catcgtccct gccgttcgcc agccgctggc
     2281 ggaggataaa tcattactgc cgtttttcag caatgagcgt gtgattcgtg ctgttggtgg
     2341 cgctggtgca ctgtctgact ggctgttgcg tcatgtcaaa tcctgccagt ggcctcatgg
     2401 tgactatcat cacagtgaaa ccgttattca ccgttatggt accggcgcaa tggtgttgtg
     2461 ctggcactgc gacaaccagc tgcgtgacca gacatccgaa tcactcgagc aacttgctca
     2521 tcaaaacctg tcagcatgga tgattgacgt catcggtcac gcaataagcg gtacgcagga
     2581 gcgtgaatta tctctggctg aattatcctg gtgggcggtc cgcaatcagg tggcggacgc
     2641 gctaccggaa gcggtattac gtcgttcgct ggggttgcgt gcggaaaaaa tccgctcaat
     2701 gtaccgtgaa agcgacatcg taccgggaga gcagaccgcc accatcatac tgaagcagcg
     2761 cacaaaaaat cttgcgccgc tgcctcacgc ccaccagcaa aacccgccac aggaaaagac
     2821 ggtggtcagc attgccgttg atccggagtc accggctcag tatctccagc gccagaaacc
     2881 acaacgggaa gagatgcctg tatacacgcg ctgggtaaaa acgcagaaat gcatgacgtg
     2941 cggtaatcag gcagatgatc cgcatcacat cattggtcat ggactgggag ggatgggaac
     3001 aaaggctgat gatttgtttg ttattccgct gtgccgtaaa tgccatagcg aactacacgc
     3061 cggggtaaaa gattttgaag aaaaacacgg cagccagctg ttgttgctga ttcgtttttt
     3121 aatgcacgcg agaaattcgg gtgtcctgaa gtggaaagca tgaatgactg aacggataga
     3181 atttgttttg ccttacccgc cgacggtgaa tacctactgg cgacgtcatg gcaatacgta
     3241 tttcatctcg gaagccggaa agcgttatcg ccgtgatgtg gcgctaattg ttcgccagca
     3301 gcggctgaaa ttaaacctgt ccggaaggct ggtgataaag attattgcag agccaccgga
     3361 taagcgccgt cgtgacctgg acaatatcct gaaagcacca ctggatgtgc tgacgcatgc
     3421 cggacttctc atagacgacg agcagtttga tgaaatcaat attgtgcgcg gtcagctcgt
     3481 tcctggtggg cggctgggga taaaaatcac agaactggag tgcgcatgaa taaccactat
     3541 ttacagtttg tgcgtgagca gctcattatc gccaccgctg atttgagtgg ggcaacaaaa
     3601 ggtcagcttg aagcctggca ggagaatgcc atgttcgata cagggcgtta caggcgaaaa
     3661 aaaatccggt accgcgatga agtgactgga aaaatgataa cgcgggataa tccaccaatc
     3721 ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc tggtcagtcc ggttgagttt
     3781 tcgacatcat cgtggcggcg ggctgttctg tctcttgaag aacatcataa agcctggttg
     3841 ttgtggtgtt acagcgggag tatttgttgg gaatatcaga tcgcgataac acagtgggcg
     3901 tggaatgaat ttaatactca atccggtacc agaaaaattg ctgggaaaac gcaggaacgc
     3961 ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag cagaactttt tggtggggaa
     4021 ggttatgaat atcaggagct ggcattactg gcgggagtga caactaaaaa ctggtccaaa
     4081 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
     4141 ttattatttg taatgagaac acgttcaaaa caaaaggcgg cattttcaaa gcaaagtgtt
     4201 gcaaaagtag attgaaaggc atatatttca tgcaaatctg atattttgcc gattttgtac
     4261 gtgatggcaa aagcaaacaa aacccgccca caagcgggtt ttgttgtgcc acttatctcg
     4321 gatagacatg gtgaatgcgc tggtggagga gataagggtg atttttgtat gcttgcaaca
     4381 ttgatttcgt aacgttatta tcctgcgccc ggccctttag ctcagtggtg agagcgagcg
     4441 actcataatc gccaggtcgc tggttcaaat ccagcaaggg ccaccaaccg ccactagcca
     4501 tcaggaaaga gcgtcaaccc tttaagttga gtgtgcgagg ttcgagtccc cggtggtggt
     4561 ccattatcgg tattctgcgt tgttagctca gcaggacaga gcaattgcct tctaagcaat
     4621 cggtcactgg ttcgactcca gtacaacgcg ctacacttat tttcccggct cgcttttgcg
     4681 ggcctttttt gtatctgcgc cacgcccggc gcataccaac cacagagcct ttcggggggg
     4741 gggagcttac ggagtggtca gtgtgacttt ctctgtgggc agatcgctcc cgggcgttgg
     4801 ctcacccacc caaaggaacg tcacgatgtt tggtattttt ggtaaaaaaa gcccgcagag
     4861 cggcaacgga aattaaaaag tttgaaaaac gcgatctggc acaggtggtg attaacgccg
     4921 cctacctggt ggcctatgca gatggtgaat gtgaggcttc ctagaaagcg aagatcgaac
     4981 aggtactgcg taatcagcct gcgctgtacg cgtttacgtc agaaattaat gcgattagcg
     5041 caaccattat cggtcagctg gatacgaact ttaaaactgg tcgtcgtgcg gcgttacgtg
     5101 agatcgagga tgtgaaacac gatacgcgtg aagcggaaga tgtgctggat gtggcggtgg
     5161 ccattgcgga ggcagacggc gaaattgagc cggaagagcg caaggtgctg gaagagattg
     5221 ccggtgttct gggtcttcgt ctggagaatt acctgtgacg gtaaaactgc gcctgactgt
     5281 ggctgcactc ctgctgtttc tggtggtgat ggtggatttc accagcagaa tcatgtcggt
     5341 gctggcggat ggggtgctgg tctgcggcat tatggtattg ctgtggccgg tgataaaaag
     5401 aaacagcctg cataatgctt gatttttttg tttgctgttt attaaaaaca cttctgcatg
     5461 gtgaatcccc ctgtgcggtg gggcaatcag caagaaggaa tatggggtaa tcgcggattc
     5521 aggtgctgat actgaattca ccgggaggca cccggcacca tgctttgcca caaaagtgtt
     5581 atttctgttt ttctcaaact atcatcgtta tccctttatt ttcggctgcg catggcgcgg
     5641 cctttttttt acgaccagcc actggcagat ggtcatcctg tgatttgatt ccgcttccgg
     5701 ctttttaact ctgttcctct acacgggaga aattcgatgt cgattaaaca ttacgatgtt
     5761 atcagggcgg cgtcgccgtc agaccttgcg gaaaagctga cacacaaact gaaagagggc
     5821 tggcagccat acggcggacc ggttgccatt acgccgtaca cactgatgca ggcggtggct
     5881 attgaaggag agccacaggt cggcccttca tctgagccgg attggtacta cgtcatcgta
     5941 ctggccgggc agtccaatgc catggcttac ggtgaagggc ttccgctgcc ggattcatac
     6001 gatgctccgg atccgcgcat taaacagctg gcgcgccgca gtacagttac gccgggtggg
     6061 gctgcctgca gatataacga tattattccg gccgaccact gcctgcatga tgtgcaggat
     6121 atgagtacgc tgaatcatcc gaaggcagac ctgagcaaag ggcagtacgg ctgtgtcggc
     6181 caggggttac atattgccaa aaaactgctc ccgtatatcc cgaataacgc ggggatcctg
     6241 ctggtaccat gctgtcgtgg tggttcggca tttacccagg gcgcggaggg gacattcagt
     6301 gcggacacgg gggccagcca ggattcggcg cgctggggtg tgggtaaacc gttatatcag
     6361 gacctgattg cgcgcaccaa agctgcatta cagaagaacc cgaaaaatgt gttgctggcg
     6421 gtgtgctgga tgcagggaga gtttgacatg agcgccgcca cccacgcaca gcaacctgcg
     6481 ctgtttacag ccatgctgac acagtttcgt gctgacctct ccgtgtttaa cgcgcagtgc
     6541 catggtggca gtgctgcaga tgtgccgtgg atttgtggtg acacgacgta ttactggaaa
     6601 aatacctacg gcacccagta caacaccatt tacggggcgt acaaaaacag ggagagtgag
     6661 ggcgtttatt ttgtgccctt catgacagac ggtaacggcg tcaataccgc cactaacgcg
     6721 ccggcagaag atccggatat tccggcatca ggatattacg gtgcggcatc gagaacgaat
     6781 ggaaaccagg tatcatcaaa ccgcccgaca catttcagtt catgggcgcg caggagcatt
     6841 attccggatc gt
//
LOCUS       DAZFRJ010000216         6810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000216 DAZFRJ010000000
VERSION     DAZFRJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.218"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(465..680)
                     /locus_tag="ACHKUN_004110"
     CDS             complement(465..680)
                     /locus_tag="ACHKUN_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662261.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            complement(767..2143)
                     /locus_tag="ACHKUN_004111"
     CDS             complement(767..2143)
                     /locus_tag="ACHKUN_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6662262.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            complement(2140..2961)
                     /locus_tag="ACHKUN_004112"
     CDS             complement(2140..2961)
                     /locus_tag="ACHKUN_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6662263.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            complement(2948..3109)
                     /locus_tag="ACHKUN_004113"
     CDS             complement(2948..3109)
                     /locus_tag="ACHKUN_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662264.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            complement(3145..3423)
                     /locus_tag="ACHKUN_004114"
     CDS             complement(3145..3423)
                     /locus_tag="ACHKUN_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO6662265.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            complement(3533..3757)
                     /locus_tag="ACHKUN_004115"
     CDS             complement(3533..3757)
                     /locus_tag="ACHKUN_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6662266.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3869..4576
                     /locus_tag="ACHKUN_004116"
     CDS             3869..4576
                     /locus_tag="ACHKUN_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6662267.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            4617..4913
                     /locus_tag="ACHKUN_004117"
     CDS             4617..4913
                     /locus_tag="ACHKUN_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662268.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHIID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
     gene            5347..5619
                     /locus_tag="ACHKUN_004118"
     CDS             5347..5619
                     /locus_tag="ACHKUN_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662269.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            5622..5984
                     /locus_tag="ACHKUN_004119"
     CDS             5622..5984
                     /locus_tag="ACHKUN_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO6662270.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(6015..6509)
                     /locus_tag="ACHKUN_004120"
     CDS             complement(6015..6509)
                     /locus_tag="ACHKUN_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662271.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
BASE COUNT         1868 a         1623 c         1484 g         1835 t
ORIGIN      
        1 tcactctcca tcgatgattt tttgggttac caataatatt tgatagtcgc cataattatc
       61 ggtagcaacg cgcagacaac tgaaaaccgt aaaacaaaac ctactcccaa tatgcgatat
      121 ccattattcc agagaacaaa actcatcatc agaaggaatc catgaaaaat cgcgacaaga
      181 aataaactac aaataaatgc atttaccatc ggtacttacc cctcgctctt aatccaataa
      241 aaaagggttt gcttcactga acactccttt attttttatg cctgtaaccc cattcttcca
      301 gcaaccttgc ggcgtaccac ccaagaaaca aaggaaagaa cattacaatg agatattccc
      361 cgccacggtc aatgttcgaa attgaccaga ttacgatgta accagtgcag gacaggaata
      421 ttacaaaccc caaaaagcta cttcttcgac tcatgctcac tccttcactt taaatccaga
      481 ctccggatta ttctgttgcg ctgaaactca ttgttgagtt tgaacaaccg tcgaaggaca
      541 cggtcacgcg gatagcgtcg tgcagcaggt gaatgctcat acaactcatc aatcggcaaa
      601 ctggacgatg aacgataccg ataccaacgc accaactctt cacgaaaatt agccctgaca
      661 agctcagcta tcgtactcat ttcttaaagc ctccaattcc ctctccccca aataaaaagg
      721 cctgcgatta ccagcaggcc tgttattagc tcagtgatgt agatggtcat acgtcagccc
      781 cttgtgcata tcgtctgcca cgcgaagcag gtgcatttga tgctgtgcaa atctgtctgg
      841 cttcatcctg gtcacatgca acaaagtgtc cgttacagaa ccgctggtaa accgtaccaa
      901 gtgggccaaa ccggtttttc gtcacaatga tttcagcaaa tggtgcggcg ctactgttct
      961 cgtcatatac cgcttcccga tagagcatga tgattgagtc tgcgtcctgc tcaatgcttc
     1021 ctgaatcacg caaatctgcg tttgtcgggc gtttgtttgg tcgcttctca acatcgcgcg
     1081 aaagctgact tagggagata acaggcgttt tcaggtcttt cgccatcgcc ttcaggcttc
     1141 ctgagatgtg agcaattgcg aggtcgttgc ggtctgcttt cggcttctca atcaggccaa
     1201 gataatccgc catgatgagt gacaggtttg gattttcctg tttgttccgc tctgcgattg
     1261 agcgaatttc ttctaccgat aaccgcgagg catcgactac ccatacatcc aaatctgcaa
     1321 gctgactcat gccgttagca acacgcgccc agccttcgtc atccatcgat gcaggatttc
     1381 gcagtacgct aaccgacatc ctcccggcgt tggcaatgct ccgctctgca atctgcaatg
     1441 cgctcatttc catcgagaaa atcaacactc cgcgccggac gtcagaacca ggaataacgc
     1501 ggcttgcaac accttcggca atcttcagcg ccagttcggt tttccccata ccaggacgag
     1561 cggcgataat caccaggtct tccgcgttca tccctccggt gatggcgtca agttcttcga
     1621 ttccggtctt cagggtatct gactcttctc cgttcctcag acgcctgtca agcgtgtcag
     1681 tgtagtcagt gatgatttcc cctaaccgta caggtttaac ctcgtcacgg ggctttctga
     1741 tggctgagag acgctttaca agctcgtcca tcgcctgact cgatgtatcg atggttccgc
     1801 tctgaattgg ttcacgcatt tcgtccatga tttccagcac cagacggcgg tgatagttat
     1861 ccgcgaccat tccggcatat cccttcaggt ttgcggcact cgggcagttt ttgctggtca
     1921 tcaggattga cgtgaaatgc tcctctccgc acgcctcggc aaccatcagc gcgtcgatta
     1981 ggtttctgtt tctcgcctgc ttacggataa cttcgaaggc tttccggtag agcggaattg
     2041 aaaacgcttc cggctccagc gttgccagaa cgtcgctggc ggttggtgtt aatccaccaa
     2101 tcagcaagcc accgataacg ctcgcttcga tatcctgtct catgcaatcc ccctgtctgc
     2161 aaacttccct tcccgaactc ccgttaacga gtcttccctc agcaggtaat caaaatctgc
     2221 cgtccagccc gtgtcgttgt ctccgaagta aaacggcttg gcctgatgca caaacgccct
     2281 gacatacgct ctgaaaccgt ccacgtttgg cgttttcagt tgcgggatga ttttcttcag
     2341 gcggcgtttt cgtttctcgt tgaccgcaac agcgtgtgga agtctgtcac cgacttcggt
     2401 gttgtaggcg ttcaggaagg attcgtagtc gattcgttct gccttgcgac gttcaggttt
     2461 aacctgccca tcgccgcccc cgttaggggg taagggggta tttgtattta ttgtcttttg
     2521 tatattgtct tttgtgttta gctgacttgg cttataccca ttagccgact cggctaatgt
     2581 tttattagct gttttagcta atgttaagct gtcctggcta atccactgag aaaccacctt
     2641 gttcactccg attttcacgc catcagcaat gaggaattta cgctcaataa gctggcgctt
     2701 ggcagcgcaa acatgagtgt gatgaatacc tgtcatggct gctatctgcg tgtttgtgag
     2761 tcgatccatc ggcttattga atccgtatgt cttgcgcatg atagcgagca tcaccttcat
     2821 ctgccggacg gttaaatcag ccatcagcag actgtcgata atctcgttag caacgcgcat
     2881 gaaaccatct tcggtatctg ccacgcgatg ctccacgacc tccagttgag gcctgtaatc
     2941 agctaactta acgacgccca tgtttcactc ctgctttggc tagtctgtaa acaccaacaa
     3001 ggcgctctgc gaacgccctg ttatttgctg cggctaccac taatccctca ggtgaatcag
     3061 ggtgtcgaat ctcttctttt tcctggtatt tcttacgacg ttttgtcata attactcctg
     3121 tggattgata cagtctttct acaatcaggc ctcaaaactg ttgccgcagt cttgaggctt
     3181 ttcttttgtc agcaccatgg ctactttctt tgccagctcc gctaattcct cgtcttcaac
     3241 accccactcc agcacagcca gaagcatggc catctttggg ataaagctgt ctttccatcg
     3301 cgaaatttgc gattcattga tccctaatgc atcagcaacc tttcgctgac cacgtacagc
     3361 aattcgattc aggatgttgc ttgtaattgc attcgctttc ttgcgagtac ttgtaagttc
     3421 catatgtaag tatttcctta acaaataaga agttatgcgc atcaacttat gcgcgttgta
     3481 ttcccgcatt tcggcgggaa tgaggaccat gactgttaaa gagcggtgtt acttatgctg
     3541 cctgattcgg ttttggaaac aggtgtggca aatcggggcg aatttcgtaa gccttgatct
     3601 gccctccagt ggcgttaacg atggcggtaa ctttctctgg agagaccaac ccgcctttca
     3661 gccatttgtg tactgctggc tgcgttacac cacacttgtc ggcaaggcgc ttttggctac
     3721 cgacaatttt caaggctcgt tgaattacta aattcatgag catacctctt gtggtcatta
     3781 cttataacca aagataactc aagttataaa aaacagcaat aacctttgtt attttacttt
     3841 ggataaccgt agttatagat ttgtgggtat gaaaacattc gcagaaagac taaatgcagc
     3901 catgagctca gcaggggtat cacaatcaca gcttgctgac atggttggaa tatctcagcc
     3961 agccatacag aagatgtcgt ccggtaaaac aaacggatct cgcaagatgg ttgaattagc
     4021 caatgcttta aaagtgcgcc ctgaatggct tagttctggt attggtgaaa tgagggatgg
     4081 tgcacatgaa gaaccatcca atgtccgtga ttcatcttta aaagctgtgg tatgggaaga
     4141 cattaaaaga aacgatgacg agtttgttgc gttgcctctt cttaacgttt cgctttcagc
     4201 tggaagcggt agctgcgagc tagaggaatc atcggagttc tctttggttt tcagaaagca
     4261 ctatctgaaa aagatgggag tatctgaaag atcagccaag ctagttaggg ttgtagggca
     4321 aagcatggaa ccaacgcttc acgatggcga tgttgttggt gttaacacgc aagataccac
     4381 aatcagagat ggtaaaacct acgctatttg ccagtctgat ttgttacgag taaaaacatt
     4441 aatcgccacc cctacatcgg tgataatcag atcaataaat cgcgaagagt acccggatga
     4501 agtaatggat agagatgaat ttcatgaaac cgtaaggatt attggcagag tattctggtc
     4561 gtctcatagt tggtaaccga taatcagaag aagacttacg gaagtgcgga gggataatgg
     4621 aatttctgat agtttttgtt gttgttttgg tcatcattct ttttgttttg ctaagcatta
     4681 gtaaaaagct atctcaaatg attgaacata gctctaatcg tgcaaaagaa gaagagcata
     4741 taattgatat aaaagagatt ctctctgata taaaaatcac attagatgaa ataaaataca
     4801 caacagatct aattgaacag tataaaatac caaccccaaa cgagagaaaa gcaatagatc
     4861 aatatcgtat tgacttagaa atcgacgaaa tgctaagcaa aagaaaagac taaaaacacc
     4921 cggcctcagc gccgggtttt cttttcctgc cgttcaccac ccaatcaacc atcctcatca
     4981 tagacaagca tcaagccaaa ggtaacacct tcaccccgca cgcaagccct caaacaccaa
     5041 tcaatcagca acatttacaa aaataaaata cctttgttat ccatcactta taacctattt
     5101 accataaaat ataaattagg ttattgacca cacctataac ctaagttatc tttaagccat
     5161 cagcaggacg ctggaagcca aacggaacag attggcaggc tctttaacat tgatgggatt
     5221 gtcccgccga aatgcgggaa ccaaagagta gttggctttg gggtgacgtg aagtgcagct
     5281 gcaagacggc aaccggaaga taagcacgcg gcgcgtcacc gccaaagtca atcatcggag
     5341 gtaaacatga cagtagtcat tacatatctg gctgacgata acgccagaaa tcgccgcaga
     5401 gcacgcagac aggctcaacg tgaacaggca atgcaagaac agcgattggc acgaaaaatt
     5461 gcgctaaagc tctctggttg cgtcagagca gataaagcag catcactcgg aagccttcgc
     5521 tgcaagaagg cagatgaatg cagtggaagt atttgcctgc caaacgtagc catttacgcg
     5581 gcaggctacc ggaaatcaaa acaactgacg gcgaggtaat tatgggtcag gaagaaaaat
     5641 atgagcttaa aaagctcatt ggagaagacg ccatagaaga aattgcagca ttaacaacag
     5701 ctataaagaa tattaggtat gcgctaaata cgcttatctc ctcatgcgac aaaaatagca
     5761 gggaattttt gatacttggc gcagctctag gaatagttga tgcggcaacg cttcacctaa
     5821 ttactcatga cgatattctt attgagccgt atgaaacatt actgcttgtc aggcaaaaaa
     5881 tggctgatgc cgcagcaaat ggagaccttc aactttacat cgacttaagg aaagtattaa
     5941 ggcgaatggt caaaactgaa ggagatatcc ccctgacaaa gtaagggggt gaagaggcca
     6001 tttcaggaat aaaattaata atttgtcagc ggcggtacat atacaggctt agggtctggc
     6061 gctctataca tccggtattc attcttacag cgaggacatg aatgcacaaa gaacccatta
     6121 gattttatag taaccgtgct tggttgaagt atcgatattt tatgttcgcc gaagcaatgt
     6181 gcacaaaggt tatgctgctg aatagaagga tcggaatgac tcttaggtcg atacacgaca
     6241 gaaccacctt cagttgtatg aagttcataa ttctcagctt gcacaacaaa catcttgatt
     6301 tttctatttt catctcgaag cttcattgcg atctccttct cggcctgata aaggccagag
     6361 agctccgcat taagcatctg taagtcagtt attctttggt gtagatcacc aatagcttta
     6421 gcgataacag catcgtctct ggtttctttt attgtcttca gcagatcata agcctgtttc
     6481 gcggcagtta taccagataa cacatccata taaattactc tcttactgta ggggtaagag
     6541 gattttacta tttttctcgc tgtaggggta cacgagaacc accgagcctg atgtggttaa
     6601 aagacaggca caatctttac taccgcaagc cacgcagtga aatgggtgtg acttgtgttg
     6661 gtcgccagaa aatgaaatta gacagcaaac cacttatttg aggtgagata tgacaaaatc
     6721 atggagcgta ccttttcctg aatcagaaac taaacatgat ggaatgcctg ttttctggag
     6781 attccaggcg acagttgaag aagatggaat
//
LOCUS       DAZFRJ010000217         6755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000217 DAZFRJ010000000
VERSION     DAZFRJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.219"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(148..633)
                     /gene="sixA"
                     /locus_tag="ACHKUN_004121"
     CDS             complement(148..633)
                     /gene="sixA"
                     /locus_tag="ACHKUN_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO6662272.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(836..2980)
                     /gene="fadJ"
                     /locus_tag="ACHKUN_004122"
     CDS             complement(836..2980)
                     /gene="fadJ"
                     /locus_tag="ACHKUN_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO6662273.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2980..4290)
                     /gene="fadI"
                     /locus_tag="ACHKUN_004123"
     CDS             complement(2980..4290)
                     /gene="fadI"
                     /locus_tag="ACHKUN_004123"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO6662274.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4470..4754)
                     /locus_tag="ACHKUN_004124"
     CDS             complement(4470..4754)
                     /locus_tag="ACHKUN_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO6662275.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5126..6466
                     /gene="fadL"
                     /locus_tag="ACHKUN_004125"
     CDS             5126..6466
                     /gene="fadL"
                     /locus_tag="ACHKUN_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO6662276.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1675 a         1818 c         1611 g         1651 t
ORIGIN      
        1 cagaagaata atttccgaat gaaagtagaa agtgttttat ttatagatgg ggagagtctt
       61 cgatagtagt gaggttgaca gatatccgct gcctgatgag caattatgcc caatgaatct
      121 acctcattgg gcataatttg gaactcatca gatagctttt gccatcttca gattacacgg
      181 gctcatctgc cagttgaatg tgccgtttcc gctttcatca agcgttacgc tggcaatggc
      241 cgaggtggta aacataggcg gcgtttcgcc cggacataac tcggcaacca gatacccgac
      301 taacggtaag tgagagatca ccagtaccga agcaacacct tcattggtca acgcctgcaa
      361 ataggcactg accagaccaa catcgccgca gggcgttaac tccggcagaa cttctgcact
      421 ggaaggcagg ttcagacaat cccctacttc ttccagtgtt tgctcggctc gcaggaacgg
      481 gctcaccaga acgcgttcga tttccacttt ttgacctttc agccagttcg ccatcaggcg
      541 agattcgtca caaccattag tggtcagagg acgaacggaa tcactggcgg catcgagggc
      601 tgcgtcgccg tgacgcatga taaaaacttg catattgcac cgcttttgtt aaccagattc
      661 gcccgttttc tttcatctga aaccagagaa agaaaacggt ggccggcatt gtgccttatc
      721 cattcaccga atgaaacgct gttttttacc tcaatggcgt aagtatagtc aatatgcgtt
      781 tatattttga ccaacactcc gccattcagc gcggattcat atagctttga ccttcttatt
      841 gcaggtcagt tgcagttgtt ttccaaaaac tttccccacg cgcgcccatc tcgaccaaac
      901 gctcgcaagg ggtaaaacgg gaaccatact gcgtggcaag tcgttgcatt attgcaacca
      961 cttcgcccgc gccgagagaa tcgatatagc ggaacggtcc accgagaaat ggcggaaaac
     1021 caatgccaaa taccgcacca atatccccgt cacgcacgct acggataacc tgctcatcaa
     1081 cacaacgtac tgcttcattc agcatcaaca tcacacaccg ttcagcaacc tgcggtgcgg
     1141 agagtcgccc ctgcccttgt gcgccaatga gcgggtaaat ggcgggatcg acctgttttt
     1201 tgcttttacg ccctttctga ccataaagat agaaaccccg accatttttt ctgcctttgc
     1261 gatcgtcgtt caaaattgaa gaaacaacat ttgcaggcgc gctaaaacgt tctccataag
     1321 cggcttccag tacaggaata attttagtcc cggtgtcgat tcctacctca tccaaaagtt
     1381 ggattggacc taccggaaaa ccaaatttca ctagcgcggc atcaatgtgc tctacccgtt
     1441 caccttcggt cagcatgcgg atagcttcat taatgtaagg cgctaagatg cgattgacgt
     1501 aaaacccggc tttgtcacgc acgacaatgg gcgttttgcc ctgttttttc gccagtttta
     1561 ccgtagtggc gatggtttgc gccgatgtac ccgcatgagg aataatctct accagcggca
     1621 ttttttccac cggactgaag aaatgcaggc caataacttg ctcaggtcgc gtggcgtgag
     1681 ctgcgatatc accaatcggt aaagatgacg tattcgaagc aaaaatggta tgagcggcgc
     1741 aattttgctc cacttccgcc accatctgtt gttttaattc gagattttca aacaccgctt
     1801 caataatcag atcgcgatgg gcaaagccgc gatagtccgt cgttccggag attaatgcca
     1861 gctgtttgtc acgttcgctg gctttgagat gacgacggcg aactttgccc tccagctgat
     1921 cccaactgta cttcagcgca tgatttatgc cctgcgggtt gatatcttta attctgaccg
     1981 gaagccccgc tttacaagca gtgacataag caataccgcc gcccatcaag ccaccaccta
     2041 aaattcccac gctgtttaat ggcgcaggcg gtgcatcact accgggatct ttcttcactt
     2101 ccgtactggc aaaaaagata ctacgcaaag cttgcgattg tggcgtcatc gccagttcgc
     2161 caaacgcccg agcttcggcg tcataaccgc tgctggtgcc ctgcgctaat cccgtttcaa
     2221 caacctccag gatgcgttct gtcgccggat aattgccttg agttttgtgt tctgttttct
     2281 tgccgaccat tttgaacagc agcgcacgac ctaacggccc cgccagaata cgctcgcgta
     2341 caggtagagg gcgggaagat gggcgatcct tcttcgccag ctcaacagcg acttccagca
     2401 aaatggagtg cggaacaacg tcatccacca gccccagctt taatgcctgt ttcgcccgaa
     2461 gttgttttcc ggtgaggatc atctctaatg ctgtgctgac gcctatcaga cgcggtaaac
     2521 gctgggtgcc gcctgaaccg ggtaacaatc caagttgtac ttcaggcaaa ccgagcaccg
     2581 ttttaggatc gtcagtacaa acgcgaccgt ggcacgccag cgccaactcc agcccaccac
     2641 ccaggcaagc gccatgaata gccgcgataa ccggaatggg caaagcatga atctccgcca
     2701 tcaactgttg gccctgccgc gccagtgctt ccgcttcttg cgccgttttg cagttgccga
     2761 tcatgttgat gtctgcgcca gcaatgaagt tgtccggttt agcggagaca aacaccacgc
     2821 ctcgcaactc tttgttttca cggagttgct taataatggc gcgcacctgc gaggcaaact
     2881 ccgccttcag ggtattcatt ttctcacccg gtacgtcgat ggtgataacg gcaatgttgt
     2941 ccagacgaac attaagggta aacgctgatg tcatttccat tattccgcct ccagaaccat
     3001 tgccgcgcca agcccaccgg cagcacaggc ggtaactaaa ccaaatccac cgccgcgacg
     3061 gcgaagttca tgcaatgtct gggtaatcat ccgtgcgccg gtcgccgcga agggatgtcc
     3121 gtaagcaatc gaaccgccaa gcacgttaaa tttgctatcg tccacttcgc cagtggcatg
     3181 tgcacgcccc agtacttcac gagcaaaacg ttcactaccc agcaactgaa tattcgccag
     3241 tgtctgagct gcaaaggctt cgtgcatatc gatcaatgtc agatcgctca ttgtcaaacc
     3301 ggcacgttcc agcgccagcg gtgttgacca ggccggaccg agcaacatgt cctgccagac
     3361 atcaatcgca gtaaatgcgt agctgcgcag ataccccagc ggcaccagcc ctaattcttt
     3421 cgcccgggat tcagtcatca ggatcaccgc tgccgcgcca tcggtcagcg gcgtactgtt
     3481 tgccgccgtt accgttccgt gtttgcgatc aaacgccggg cgcagctttg cgtaatcggc
     3541 aagcgaggaa ttaccgcgaa tattgttgtc ttcgacaagc ggttgtttat aaggagggat
     3601 aaaggcagtc atcacctctt ctttgagttt tccgtctgac catgcctgag cggcacgctg
     3661 atgcgaacgg tgcgctaatg catcttgctg ttctcgggtg atgccgtagg ttttcgccat
     3721 ttgttcggcg gtgtcgccca tccgcaagcc agtagaatat tctgctaccg caggcggtac
     3781 gggcattaag tcgcgcaaac gcaggcgaga gaagagtttc agtcgctggc tcatggttcg
     3841 agctttgttg acatcaacca gcacgcgcgc cagtttttta ctgacgccaa ttggcaatac
     3901 ggaagaggaa tctgccccac cggcaatccc cgctcggata gttcccgcca tcaggctttc
     3961 tgcgacgttt gcaaccgcct ggaaactggt agcgcaagcg cggctgacgc tgtaagcatc
     4021 ggtatgcaca ttcattccgg taccgagaac aatttcacgc gcaatgttgg gggcttcagg
     4081 catttgtacg acctgaccaa agaccagctg ttcaatcact tcagcgggga tctcgctgcg
     4141 tgccagcagt tcgcctacca ccatcttccc taaatcaacc gcgggaatgc catgaaaagc
     4201 cgtcgcctgg cgggcaaaag gcgtacgtaa accgctaaca atggcgatac gatcgccctg
     4261 gcgggtaacc agcggtaaaa cctgacccat aacactcccc tgtaaaaaaa taaataaagt
     4321 ggtctgacct gatcatagtc ttaaccattt ttttacattt agccaagcgg agaaaaagga
     4381 aagtgggagc tatgacacag tgaagacagg agaaagaaaa gaaaacgccc ctgccagcaa
     4441 ctgacagggg cgtacgtacc cgagaggaat taacgcagac ccagctggaa gatcagcatt
     4501 tcagcttcgc aggcaaaagt gaaatcgata tccaggcgca caccgtcaga ctcttcagtg
     4561 aaagtcgggg tgattttaca cggttcggat tccacgctac gggctttttc agtcagcgcc
     4621 gccagcgttt gttctgcttc tgcgcggctt gcaaacacgc ggctgtaaga cgcggtgcag
     4681 tcggagttgt ccataatggt gccaacatcc atacagcagc aaaccggggt ttcatcagca
     4741 ctacatttac tcatcgttga tttcctctgt atgtgcaccc aaggtgccag ataaacgttg
     4801 tggatatttt acgcttccgg aaagtgctgc tccagttgtt aattctgcaa aatcggataa
     4861 gtgaccgaaa tcacacttaa aaatgatcta aaacaaaatt cacccgaatc cgcgagtgcg
     4921 caacctccaa attttgccag ctggatcgcg tttcttagat catatttgaa aaaagatgga
     4981 aacatacttg caacattcca gctggtccga cctatactct cgccactggt ctgatttcta
     5041 agatgtacct cagaccctac acttcgcgct cctgttacag cacgtaacat agtttgtata
     5101 aaaataaatc attgaggtta tggtcatgag ccagaaaacc ctgtttacaa agtctgctct
     5161 cgcagtcgca gtggcactta tctccaccca ggcctggtcg gcaggctttc agttaaacga
     5221 attttcttcc tctggcctgg gccgggctta ttcaggggaa ggcgcaattg ccgatgatgc
     5281 aggtaacgtt agccgtaacc ccgcattgat taccatgttt gaccgcccga cattttctgc
     5341 gggtgcggtt tatattgacc cggatgtgaa tatcagcgga acgtctccgt ctggtcgtag
     5401 cctgaaagcc gataacatcg cgcctacggc atgggttccg aacatgcact ttgttgcacc
     5461 gattaacgac caatttggtt ggggcgcttc tattacctct aactatggtc tggcaacaga
     5521 gtttaatgat acttatgcag gcggctctgt cgggggtaca actgaccttg aaaccatgaa
     5581 cctgaactta agcggtgcgt atcgcttaaa taatgcatgg agctttggtc ttggtttcaa
     5641 cgccgtctac gctcgcgcga aaattgaacg tttcgcaggc gatctggggc agttggttgc
     5701 tggtcagatt atgcaatctc ctgccgggca aactcctcaa gggcaagcat tggcagctac
     5761 cgccaacggt atcgacagta ataccaaaat cgctcatctg aacggtaacc agtggggctt
     5821 tggctggaac gccggaatcc tgtatgaact ggataaaaat aaccgctatg cactgaccta
     5881 ccgttctgaa gtgaaaattg acttcaaagg taactacagc agcgatctta atcgtgcgtt
     5941 taataactac ggtttgccaa ttcctaccgc gacaggtggc gcaacgcaat cgggttatct
     6001 gacgctgaac ctgcctgaaa tgtgggaagt gtcaggttat aaccgtgttg atccacagtg
     6061 ggcgattcac tatagcttgg cttacaccag ctggagtcag ttccagcagc tgaaagcgac
     6121 ctcaaccagt ggcgacacgc tgttccagaa acatgaaggc tttaaagatg cttaccgcat
     6181 cgcgttgggt accacttatt actacgatga taactggacc ttccgtaccg gtatcgcctt
     6241 tgatgacagc ccagttccgg ctcagaatcg ttctatctcc attccggacc aggaccgttt
     6301 ctggctgagt gcaggtacga cttacgcatt taataaagat gcttcagtcg acgttggtgt
     6361 ttcttatatg cacggtcaga gcgtgaaaat taacgaaggc ccataccagt tcgagtctga
     6421 aggtaaagcc tggctgttcg gtactaactt taactacgcg ttctgataac gcgttcgcct
     6481 ggataaagtc acctgcatag caggtgactt taactccccc acttcaccga aagtagtgtc
     6541 ctcattgctt acctcctgag ttttgcaaac agcctgatgg cagcttgccc tttcaaatca
     6601 ataagcggta tccacagaca aatctgaagg gaaaggcata ttttcaggcg ttctgctcgt
     6661 ccttctcaaa gagtttactt ttctgaattt ccaggatact cacccccctg gctattgtgc
     6721 gctcatacac tcaaattaaa gataggttct aaata
//
LOCUS       DAZFRJ010000218         6735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000218 DAZFRJ010000000
VERSION     DAZFRJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.220"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKUN_004126"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKUN_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO6662277.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKUN_004127"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKUN_004127"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6662278.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKUN_004128"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKUN_004128"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO6662279.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKUN_004129"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKUN_004129"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6662280.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKUN_004130"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKUN_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO6662281.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1469 a         1829 c         1828 g         1609 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaat
//
LOCUS       DAZFRJ010000219         6713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000219 DAZFRJ010000000
VERSION     DAZFRJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.221"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            232..474
                     /gene="yjbE"
                     /locus_tag="ACHKUN_004131"
     CDS             232..474
                     /gene="yjbE"
                     /locus_tag="ACHKUN_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO6662282.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            588..1226
                     /locus_tag="ACHKUN_004132"
     CDS             588..1226
                     /locus_tag="ACHKUN_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO6662283.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1223..1960
                     /locus_tag="ACHKUN_004133"
     CDS             1223..1960
                     /locus_tag="ACHKUN_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6662284.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1960..4056
                     /locus_tag="ACHKUN_004134"
     CDS             1960..4056
                     /locus_tag="ACHKUN_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6662285.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4103..4381)
                     /locus_tag="ACHKUN_004135"
     CDS             complement(4103..4381)
                     /locus_tag="ACHKUN_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662286.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4382..4525
                     /locus_tag="ACHKUN_004136"
     CDS             4382..4525
                     /locus_tag="ACHKUN_004136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662287.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4651..5061
                     /gene="psiE"
                     /locus_tag="ACHKUN_004137"
     CDS             4651..5061
                     /gene="psiE"
                     /locus_tag="ACHKUN_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO6662288.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5105..6580)
                     /gene="xylE"
                     /locus_tag="ACHKUN_004138"
     CDS             complement(5105..6580)
                     /gene="xylE"
                     /locus_tag="ACHKUN_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO6662289.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1672 a         1689 c         1785 g         1567 t
ORIGIN      
        1 tttatttcgg aaaatacgca gattaattgc ttttgttttt tattttaagt ttatgatttt
       61 tattgttatt taaatataag ttgaaactta tatttgatat tcattccaat tatcctaaaa
      121 cgccatcgct aattccccgc gccgtaattc gcatgcttta gttgtgtata ctcgatcccg
      181 cccgaaatgt ttttgggtaa atctccattc attcaatgaa gggaaattgt tatgaaaaaa
      241 gttctgtatg gcatttttgc catatctgcg cttgcggcga cttctgcgtg ggctgcacct
      301 gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg tttcggcggg gagttcctcc
      361 gctaccagcg tcagcaccgt aagctcggcg gtgggtgtcg cgctcgcggc aaccggtggc
      421 ggtgatggtt ctaataccgg gaccaccaca accacgacca ccagtaccca gtaataaagt
      481 atgtatcccc aaaataattt gagtcattgc atctgtggct tgaagtatga agggatttac
      541 cataaccaca ctccggtgtg gttattctgc ccctctggag aagagtcgtg aagcgacctg
      601 cactcattct tatctgcctg ctattacagg cctgttcagc cacgactaaa gagctgggca
      661 attcactgtg ggacagtctg ttcggcacgc caggcgtaca gctgacggac gatgatattc
      721 aaaatatgcc ctacgccagc cagtacatgc agcttaatgg cgggccgcaa ttgtttgtgg
      781 tgctggcctt cgctgaagac ggacaacaaa aatgggtcac tcaggatcaa gccactcttg
      841 tcacacaaca tggtcgtctg gtgaagactt tgcttggcgg cgacaacctg attgaagtga
      901 ataaccttgc cgccgacccg ctgattaaac ccgcacaaat tgttgatggt gcaacatgga
      961 cgcgcacgat gggctggacc gagtaccagc aggtacgcta cgccaccgca cgctcagtct
     1021 tcaaatggga tggcaccgat accgtcaaag tcggcagcga tgaaaccctg gttcgcgtgc
     1081 tggacgaaga agtctccacc gaccaggcgc gctggcataa ccgctactgg atcgacagcg
     1141 aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga ttatttcccg gtgaaaacca
     1201 ctctcatcaa ggcggcaaaa caatgattaa acaaactttt gtcgcgttgc ttttgagtgt
     1261 gggagcgtca tcggtcttcg cggcaggaac cgtcaaggtg ttcagcaatg gcagcagtga
     1321 ggccaaaaca ctaacaggcg cagagcattt aatcgatctg gtaggccaac cgcggctggc
     1381 aaacagctgg tggcccggtg cggtgattag cgaagagctg gcaacggcgg cagcattgcg
     1441 tcagcagcag gcgttgctga cacggctggc agaacagggc gcagattcca gcaccgacga
     1501 tgccgccgcc attaacgcct tacgccagca aatccaggca ttgaaagtga caggcaggca
     1561 aaaaatcaat cttgatcccg atatcgtccg cgttgccgaa cgcggtaacc cgccgttgca
     1621 gggcaactac acgctgtggg tcggaccacc gccgtccacg gtcacgttgt tcgggcttat
     1681 cagccatcct ggcaatcagc cattcactcc aggtcgcgac gtggcgagct atctctccgg
     1741 gcagaacctg cttagcggtg cggatcgcag ctacgcgtgg gtcgtttacc cggacggacg
     1801 cacgcaaaaa gcgccggtgg cttactggaa caagcgtcac gtagagccga tgcccggcag
     1861 cattatttat gttggcctcg cggactccgt ctggagtgag acgcctgatg cccttaacgc
     1921 cgacattctt cagactctga cgcagcggat accccaataa tgaaaaaaag acatctgctt
     1981 agcttactgg cgctgggcat tagcatggct tgctacggcg aaacatatcc tgcgcccatt
     2041 ggcccgtcgc agtcggattt cggtggcgta ggattattac aaactcccac cgcgcgtatg
     2101 gcacgggaag gggagttgag tctgaactat cgcgataacg atcagtaccg ttattactca
     2161 gcttcagtgc aactcttccc gtggctggaa acaacgctgc gctacaccga cgtgcgcacc
     2221 cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga taaagccttc
     2281 gatctcaaac tgcgtttgtg ggaagagagt tattggctgc cgcaagtggc ggttggtgcg
     2341 cgggatatcg gcggtacggg gctgtttgat gcggaatatc ttgttgccag caaagcctgg
     2401 gggccgttcg attttacgct cggcctgggc tggggatatc tgggcaccag cggtaatgtg
     2461 aaaaatccgc tctgttcagc cagtgataaa tattgctatc gcgataacag ctacaaacag
     2521 gcgggatcta tcgacggtag ccagatgttc cacggtcctg cctcactgtt tggcggcgtg
     2581 gaataccaga cgccctggca accgctgcgc ctgaaactgg agtatgaagg caataattat
     2641 cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg tgcgatttat
     2701 cgcgttaccg attgggccga cgttaacctt agctatgaac gtggcaacac ctttatgttt
     2761 ggcgttacgt tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga taacgcccgc
     2821 ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc attcggtggt ggcgaatcag
     2881 ttaacgttgc tgaaatacaa tgccggactt gccgatccgc agatccaggc gaaaggcgat
     2941 acgctgtatg ttaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg gatcatccgt
     3001 gcgaatcgga tcgtgatgaa cgatctgccg gatgggatca aaacgatccg cattacagaa
     3061 aatcgcctta acatgccgca ggtgacgacg gaaaccgatg tcgccagctt gaaaaatcat
     3121 ctcgccgggg agccgctggg ccatgaaacg aaggtggcgc aaaaacgcgt cgagccagtg
     3181 gttccgaagt ccaccgagca gggatggtat atcgacaaat cacgctttga tttccatatc
     3241 gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact tttacatgta tcagctgggc
     3301 gtgatgggaa cggcagattt gtggctgacg gaccatctgc tgaccaccgg cagcctgttt
     3361 gcaaatcttg ccaacaacta cgacaagttt aactacacca atccgccgca ggactcgcac
     3421 ttaccgcgcg ttcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta tgtgaataac
     3481 ctgcaagcca actacttcca gcatctgggc aacggcttct acggtcaggt ctatggaggt
     3541 tatctcgaaa ccatgtttgg cggtgccggg gcagaagtgt tgtatcgtcc gctggacagc
     3601 aactgggcgt ttggtctgga tgccaactac gttaaacagc gcgactggcg tagtgcaaaa
     3661 gatatgatga aattcaccga ctacagcgtg aaaaccggac atctgaccgc ctactggacg
     3721 ccatctttcg cccaggacgt gttggtgaaa gcgagcgtcg ggcagtatct ggcaggggat
     3781 aaaggcggca cgctggagat cgccaaacgc tttgatagcg gcgtggtggt tggcggctat
     3841 gccacgatca cgaatgtttc gaaagaggag tacggcgaag gggacttcac caaaggcgtg
     3901 tatgtctctg tgccgttgga tctcttctcg tctggcccga cacgcagccg tgcggcgatt
     3961 ggctggacgc cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt ccagttgtat
     4021 gacatgacca gcgaccgtag cgtcaatttc cgctaagtca tgggaaaggt gccagttttc
     4081 gcaatcactg gcaccctcat tcttaatggc ctgttgagcc atcaggaacg cagttcaccc
     4141 ccggtttgcg atctggtgtg taaaggcgaa aacattctgt cttcctgtcc gtcgatatcg
     4201 gttgcagatc tggttgcctg agcgactggg ccatcattgg gctggagaca ttgctttgcg
     4261 gtaggtcggc tttatcagca gtaccgagcg gaccagcata agcaggaaga acagagactg
     4321 ataacatcaa agcagcaaaa taaggcttca tttttaccac ctttatcagg ttacgtttca
     4381 tttgttccag aggaacattg tcgatttttc gcgcattgct ggtggctggg aatcacctga
     4441 atgggtgatt tttgaattac cggctttggt gcggtttgtc ttgccggatg cgccgccagg
     4501 cgcggcttat ccggcctacg ggtaggtata tccggctttg gtggaggcgc gctccaaatc
     4561 caggttgaac aaaacataca caaaaaatat agatctccgt cacatttttg cgttatacag
     4621 gaagctcgcc actgtgaagg aggtactgct atgacgtcac tctctcgtcc gcgcgtggag
     4681 tttatctcca ccattttgca gaccgtactc aatcttggcc tgttgtgcct cggcctgatt
     4741 ttggttgtct tcctcggcaa agaaacggtg catctggctg atgtgctgtt cgcgccagaa
     4801 caaaccagca aatatgagct ggtagaaggg ctggtggttt actttctcta tttcgaattt
     4861 atcgcgctga ttgtgaagta ctttcagtcc ggttttcact tcccgctacg ttactttgtc
     4921 tatattggga tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc gccactggat
     4981 gtattgatct actcggcagc gatcctgtta ctggtgatca ccctgtggct gtgtaattcg
     5041 aaacggctga agcgggagta aaaagacagc acgccgaact ggcgcggcgt gctggacagg
     5101 aagattacag tgtagcagtt tgttgtgttt tcttcgtttc cggttcccag agggcttcca
     5161 gctcctcaag ggttttacct ttggtttccg ggacaaattt ccacataaat agtgctgcca
     5221 gaacgcccat acaaccgtaa atccagtagg agaaaccgtt gtggaaatgg gtcaccagcc
     5281 aggagttttt gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc agccactggg
     5341 ccgccaccgc gattgccagc gctttaccac gaatagcatt cgggaagatt tccgacagca
     5401 gtacccagca taccggaccc caggacatgg caaaggcagc aacatagaac agcatcgaca
     5461 gtagcgccac aatacccggt gcctgagtgt aaaacgcggt accgaggcta aacataccga
     5521 ttgccattcc gagcgcgccg ataatttgca gtggcttacg accaaattta tccaccgtca
     5581 taattgccag aacggtgaag gtgaggttga taactccgac aataatggtc tgcaacagcg
     5641 cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac aacaccacat
     5701 tgatgccgac aaattgctgg aagatggaga gcataacgcc gataacaatc acgcccacgc
     5761 cgaacatcag cagacgacca ccggttttgc gtccatgatc cagggagtgt ttaatttcct
     5821 gcaccgcctg ggctgccaaa gcgttgccca taattttgcg caggatgcct tccgcttgtt
     5881 cttgcttacc gcgtgacatc agccagcgag ggctttccgg cacggtataa agcagcatta
     5941 agaacagcag cgcagggata cattccgaag caaacatata acgccagccg tcagtattca
     6001 gccaggtggc atcgccggaa cgggcaataa aatagtttac gcagtaaact aaaagttggc
     6061 cgaaaataat cgcaaactgg ttaaaagaga ccagcttccc acgaatatgt gcaggagcca
     6121 gttccgcaat atacattggt gagagcattg aggctaaacc aacaccaata ccgccaataa
     6181 tgcgataaat aacaaattcc gggacataac ctgccagata aacaggcact gtgttgtccg
     6241 ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa ataaaaaaca
     6301 ggacagcagc aatcttaagt gaatcacgac gaccgaagcg gttactgcaa taaccaccga
     6361 gggcaccgcc gatgatgcaa ccaatcagag cgctggctac gcaaaaccct aacagggagt
     6421 tggcagcgga ttcacttaag ttttgtggag caacaaagac ggtatggagt gactcaacag
     6481 taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta gcgactaagg
     6541 taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc cttagaccat
     6601 tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc aattatcatt
     6661 ttttacaatg cgtttacgta atgtcttttc tgtgatctta attgtgataa tta
//
LOCUS       DAZFRJ010000220         6696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000220 DAZFRJ010000000
VERSION     DAZFRJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.222"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..245
                     /locus_tag="ACHKUN_004139"
     CDS             <1..245
                     /locus_tag="ACHKUN_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662290.1"
                     /translation="KKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTEWPVDESD
                     DVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            258..701
                     /locus_tag="ACHKUN_004140"
     CDS             258..701
                     /locus_tag="ACHKUN_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662291.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            1252..1458
                     /locus_tag="ACHKUN_004141"
     CDS             1252..1458
                     /locus_tag="ACHKUN_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO6662292.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            1534..1830
                     /locus_tag="ACHKUN_004142"
     CDS             1534..1830
                     /locus_tag="ACHKUN_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6662293.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1836..2621
                     /gene="bet"
                     /locus_tag="ACHKUN_004143"
     CDS             1836..2621
                     /gene="bet"
                     /locus_tag="ACHKUN_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO6662294.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2618..3298
                     /locus_tag="ACHKUN_004144"
     CDS             2618..3298
                     /locus_tag="ACHKUN_004144"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6662295.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3295..3477
                     /locus_tag="ACHKUN_004145"
     CDS             3295..3477
                     /locus_tag="ACHKUN_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO6662296.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            3450..3641
                     /locus_tag="ACHKUN_004146"
     CDS             3450..3641
                     /locus_tag="ACHKUN_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO6662297.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            3718..3933
                     /locus_tag="ACHKUN_004147"
     CDS             3718..3933
                     /locus_tag="ACHKUN_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6662298.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            4032..4250
                     /locus_tag="ACHKUN_004148"
     CDS             4032..4250
                     /locus_tag="ACHKUN_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6662299.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            4298..4576
                     /locus_tag="ACHKUN_004149"
     CDS             4298..4576
                     /locus_tag="ACHKUN_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6662300.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            4548..4613
                     /locus_tag="ACHKUN_004150"
                     /pseudo
     CDS             4548..4613
                     /locus_tag="ACHKUN_004150"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4775..5938
                     /gene="intD"
                     /locus_tag="ACHKUN_004151"
     CDS             4775..5938
                     /gene="intD"
                     /locus_tag="ACHKUN_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO6662301.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(5954..6030)
                     /locus_tag="ACHKUN_004152"
     tRNA            complement(5954..6030)
                     /locus_tag="ACHKUN_004152"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5994..5996),aa:Arg,seq:tct)
     gene            6273..>6696
                     /gene="fimZ"
                     /locus_tag="ACHKUN_004153"
     CDS             6273..>6696
                     /gene="fimZ"
                     /locus_tag="ACHKUN_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO6662302.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCS"
BASE COUNT         1967 a         1554 c         1602 g         1573 t
ORIGIN      
        1 ttaaaaaatc aaataactgt gtcgatgcat acgccgacgc gtttggctac tcttcgtttg
       61 gtgacggctc tcagcgccta ggttcaattt ctttttttca taatgtaact gaatggcctg
      121 ttgatgaaag tgacgatgtt gagaacataa agtacaacat tgcaaccatt agaatgcccg
      181 aagaattgat gcttaaattg gctgacttca ttcgcgacca gcatgacaaa gctaaaacaa
      241 actaatcgta attaattatg aaacacgatt attacgaagc tccaaaccta aatgataaaa
      301 ttgcaaaggc ggcagaatca ttttgtttgc actccagtgc gtcttcccag ctagcaggta
      361 tatctggaaa aagatgggac atgctacaat caagtggtag cagtgggtct gggagtgata
      421 acatgcctga aaaagttgca aaactggagt ccgatgttgc tcatatcaaa cgagacgttg
      481 atgaacttaa aacagacgtt aaagctatcg acaaaaatat gataactatt cttgcacgtc
      541 tggactctat aaaagaatcc ttaacaaaca agccatcaag tgatgctgtc gatagaaaga
      601 tttcagacgc aaagcttgcg gtattgcttg gtgttccagc aatcatcgct gcaggaacag
      661 gactttataa gctatcaatg tactttttct ttagtgctta agaaaatgct cgcacccttg
      721 cattaggtaa agcgattttt cattccccat taccctcgct cttaaccaat catctaaacc
      781 cggccaccgt gccgggtttt cttttgccct cccctcatca tacaccgttc aaaaaaacca
      841 ccacgacctc gcttcagtta tcgctatgcg atgcaagtca caaaataaat ccatcttaaa
      901 tacaaccagt tatatctaaa acaaccaaca aaacaacttt tgttgttgac gataaaacaa
      961 ctatagtttt aaatgagttc atcgcaacaa cacaacgata cggcaatcac ctgattcacc
     1021 gttgcgatga ccgcttagat ccgcagcttg aatttcagca ggcttcgggg agtgcgaggg
     1081 atgaaacgga cgcgtgaacg tcggtgtgac cagctgaaat caactcaaca tttcatacct
     1141 tagtcgcttc aacgaggcgg cttagttatg acaaccggcg gccatccacc gcctgaatac
     1201 gcgcagaagt ctttatatgt tcagcagccc agcttacggg caggagtttt tatggttcat
     1261 caacattacg gaacgcagac cgttaatcgc ggtgcggtca tgccaggaat gctggtcaaa
     1321 cgcaaagatg gtacctggac tgcatcagct aatttacgcg gacgacttta tctgcatcgc
     1381 ggcattgagc gcacttatac ccgtgacttg ctcgtggaag tttttctcga cggacgcggc
     1441 aacgctctga atcactaatc ccctttcctg ttttccgaat cagcctggca ttccgcgggc
     1501 gattttttca cagccatttt caggagttca gccatgaacg cttattacat tcaggatcgt
     1561 cttgaggctc agagctgggc gcgtcactac cagcagatcg cccgtgaaga gaaagaggca
     1621 gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc
     1681 gatcatttgc aacgccacgg ggccagcaaa aaagccatta cccgtgcgtt tgatgacgat
     1741 gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac cattgctcac
     1801 caccaggttg atattgattc agaggtataa aacggatgag tacagcactc gcaacgctgg
     1861 cagggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa ctgatcacca
     1921 ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc gcattgttga
     1981 tcgtcgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc ttccctgata
     2041 agcagaacgg cattgttccg gtggtgggcg ttgatggctg gtcccgcatc atcaatgaaa
     2101 accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca tgccggattt
     2161 accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa tgccgccgcg
     2221 aaccattcaa aacccgcgaa ggcagagaaa tcacggggcc gtggcagtcg catcccaaac
     2281 ggatgttacg gcataaagcc atgattcagt gtgcccgtct ggccttcgga tttgctggta
     2341 tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact gcagaacgtc
     2401 agccagaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt aacactctgc
     2461 tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc cagatatttc
     2521 gccgcgacat tcgcgcatcg tcagaactga cacaggccga agcagtgaaa gctcttggat
     2581 tcctgaaaca gaaagccact gagcagaagg tggcagcatg ataccggaca ttatcctgca
     2641 gcgtaccggg atcgacgtga gagctgtcga acagggggat gatgcatggc acaaattacg
     2701 gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaagcccc gctcaggaaa
     2761 gaagtggcct gacatgaaaa tgtcctactt ccacaccctg ctggctgagg tttgcaccgg
     2821 tgtggctccg gaagttaatg ctaaggcgct ggcctgggga aaacagtacg agaacgacgc
     2881 cagaaccctg tttgaattca cttccggcgt gaatattact gaatccccga tcatctatcg
     2941 cgacgaaagt atgcgcaccg cctgctctcc cgatggttta tgcagtgacg gcaacggcct
     3001 tgaactgaaa tgcccgttta cctcccggga tttcatgaaa ttccggctcg gtggtttcga
     3061 ggcaataaaa tcggcttaca tggcccaggt gcagtacagc atgtgggtga cgcgaaaaga
     3121 tgcctggtac tttgccaact atgacccgcg catgaagcgt gaaggcctgc attatgtcgt
     3181 gattgagcgg aatgaaaagt acatggcgag ttttgacgag atggtgccgg agttcatcga
     3241 aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat ggcgatgacg
     3301 catcctcacg ataatatccg ggtaggcgcg atcactttcg tctactccgt tacaaagcga
     3361 ggctgggtat ttcccggcct ttctgttatc agaaatccac tgaaagcaca gcggctggct
     3421 gagaagataa ataataaacg ggaggcggta tgcacaaagc atctcctgtt gagttaagaa
     3481 cgagtattga gatggcacat agccttgctc aaattggagt caggtttgtg ccaataccag
     3541 tagaaacaga cgaagaattt catacgttag ccacatccct ttcacaaaag ctggaaatga
     3601 tggtggcgaa agcagaagca gatgagagag accaggtatg acaaccactg aatgcatttt
     3661 tctggcagcg ggcttcatat tctgtgtgct tatgcttgcc gacatggggc ttgttcaatg
     3721 acacctcagc aagaaaacgc ccttcgcagc attgcccgtc aggctaattc tgaaatcaaa
     3781 aaagccagac agcattttcc ggataaaaac gtcgatgaca tttgccgtag cgtactaaag
     3841 aagcaccgcg aaacggtaac gctgatggga ttcacaccga ctcatttaag cctggcgatc
     3901 ggcatgttaa acggcgtctt taaggaacgg tgaacatgaa aagcaaaatc atcagggagc
     3961 tacaggctcc ttttttattg ttcgcattca tcctcaagcg tattaaccaa caattcaggg
     4021 attaatggaa gatggcagac atcattgatt cagcatcaga aatcgaagaa ttacagcgca
     4081 atacagcaat aaaaatgcgt cgtcagaact accagactgt atccgcaact cattgttgtg
     4141 agtgtggcga tccgatagat gagcgaagac gcctggctgt tcagggttgt cggacttgtg
     4201 caagttgcca ggaggagatc gaacttaaga acaaacaatg gggactgtga tggcctcaaa
     4261 gcagcaaatt tcaacatcgt ccaactgagg tgtaaaaatg ttcagaatca tttttcctaa
     4321 cacctggtac gtcgaccacc acggcactcc ctgcaaaatc ctgcgttcta cccacaacaa
     4381 agttcactac atccgaaaag gcagaacatg tatcgccagc atgttccgct ttaatcatga
     4441 ctttgaacct gtgaataaag ctgatgcaga tcggatagca gaagagatcg aaacggcaga
     4501 acacattaag aagttacgtg acatgcgttc aaaaagcaga ggtaaccatg gaatcataca
     4561 gcctcacact cgatgaggcc tgtcagtttc ttaagatatc cagaccaacc gccaccaact
     4621 ggatacgaac aggccgccta caggcaacac gtaaagatcc aaccaagcca aaatctcctt
     4681 acctcacaac acggcaagcc tgcattgcgg cgcttcagtc tccgctgcat actgtccagg
     4741 tgagcgcggg tgatggcata acagaggaaa gaaaatgtca ctcttccgca gaaatgaaat
     4801 atggtatgcc tcgtattcgc tcccgggcgg gaaacgaatt aaggaatctc ttggcacaaa
     4861 ggacaagcgg caagctcagg agttgcacga caagcgaaaa gcagaactct ggcgagtaga
     4921 aaagctaggg gatttacctg atgtcacttt tgaagaggcc tgcctaagat ggcttgagga
     4981 aaaagctgat aaaaaatctc tcgattcaga taaaagccgg attgagttct ggcttgaaca
     5041 ttttgagggt ataaggctta aagatatctc ggaggcaaag atttactctg ctgtaagcag
     5101 aatgcataac agaaagacga aagaaatatg gaaacagaaa gttcaggccg ccatcaggaa
     5161 aggtaaagaa ccgcctgttt atgaaccaaa gccagtatca actcagacaa aggcaaagca
     5221 tcttgccatg ataaaggcca ttctccgtgc tgcagaacgc gactggaagt ggctggaaaa
     5281 agcgcctgtc atcaagatac cagcggtcag aaacaagcga gtcagatggc tggaaaagga
     5341 ggaagcaaaa cgccttattg atgagtgccc cgaaccactg aaatctgtcg tcaagtttgc
     5401 gctggcaact ggtctgagaa agtcgaacat cataaatctg gaatggcaac aaatcgacat
     5461 gcagcgacga gttgcctggg tgaatccaga agagagcaaa tcaaaccgcg ccattggtgt
     5521 ggcgctgaac gataccgcct gtaaagtgtt gcgtgatcaa ataggcaagc atcacaaatg
     5581 ggtgtttgta cataccaagg cggctaagcg agcagatgga acatcaacgc ctgcggtcag
     5641 gaagatgcgc atcgacagca agacatcatg gctatcagct tgtcgtcgtg caggaattga
     5701 agatttccgt ttccatgacc tcagacacac ctgggcaagc tggctgattc agtcaggcgt
     5761 cccattatca gtgcttcagg aaatgggcgg atgggagtcc atagaaatgg ttcgtaggta
     5821 tgctcacctt gcgcctaatc atttgacaga gcatgcgagg aaaatagacg acatttttgg
     5881 tgataatgtc ccaaatatgt cccactctgg aattatggag gatataaaga aggcgtaact
     5941 gattgaattg taatggcgcg ccctgcagga ttcgaacctg cggcccacga cttagaaggt
     6001 cgttgctcta tccaactgag ctaagggcgc gttgataccg caatgcggtg taatcgcgtg
     6061 aattatacgg tcaacccttg ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa
     6121 cgaccgcgtc tgattttctg atttatttcg ctatagcggc aaacaaacac acaccgctgc
     6181 gcgtctgaat caagaaaacc cgtattttca tgtatcaaag tacaatttac cgccctaacg
     6241 gaaaattgtc cgctcctatg agactggtaa ctatgaaacc aacgtcggtg atcattatgg
     6301 atactcatcc tatcatcaga atgtctattg aagttctgtt gcaaaaaaac agtgaattgc
     6361 agattgtcct gaaaacggat gattaccgca taaccatcga ttatctccga acccgtcctg
     6421 ttgatttaat cattatggat atagacttgc ccggaacaga cggttttacc ttcctgaaaa
     6481 ggatcaaaca aatccagagc acagtgaaag tgttattttt atcatcgaaa tcagaatgct
     6541 tttatgctgg cagagcgata caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc
     6601 agaatgatat ttttcatgcc gttcagatga tcctctccgg atacacgttt tttcccagcg
     6661 aaacgcttaa ctatataaaa agcaataaat gtagta
//
LOCUS       DAZFRJ010000221         6684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000221 DAZFRJ010000000
VERSION     DAZFRJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.223"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            679..813
                     /locus_tag="ACHKUN_004154"
     CDS             679..813
                     /locus_tag="ACHKUN_004154"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662303.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            1414..2235
                     /locus_tag="ACHKUN_004155"
     CDS             1414..2235
                     /locus_tag="ACHKUN_004155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6662304.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            2235..3341
                     /locus_tag="ACHKUN_004156"
     CDS             2235..3341
                     /locus_tag="ACHKUN_004156"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6662305.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            3435..5156
                     /gene="cptA"
                     /locus_tag="ACHKUN_004157"
     CDS             3435..5156
                     /gene="cptA"
                     /locus_tag="ACHKUN_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6662306.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            5230..6228
                     /gene="lpxM"
                     /locus_tag="ACHKUN_004158"
                     /gene_synonym="msbB"
     CDS             5230..6228
                     /gene="lpxM"
                     /locus_tag="ACHKUN_004158"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6662307.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            6281..6469
                     /locus_tag="ACHKUN_004159"
                     /pseudo
     CDS             6281..6469
                     /locus_tag="ACHKUN_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6532..>6684)
                     /locus_tag="ACHKUN_004160"
     CDS             complement(6532..>6684)
                     /locus_tag="ACHKUN_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO6662308.1"
                     /translation="GALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWR
                     IALPAE"
BASE COUNT         1736 a         1443 c         1650 g         1855 t
ORIGIN      
        1 atacccctaa ttcaactaat tggaggcatt accatgcatt agtgcaagcg ccttgtggat
       61 tcttttgcta atggcggaaa catattgtac aatattgcat ttatccctca ataaataaat
      121 gagttatcag attgcaaaaa tctgttatca aactaattca aataaaaaga gattgatacg
      181 accaacgtac acaaaaattt tcgatattcg aactaaataa cagctcctaa taccgctaca
      241 ctttagctat ccagtctgcg cctcagaaat ggacgatgcc gttgaaggac tggtgtatgg
      301 caatgagccg ctttattatc gaattcggtg accgcttgga cggtcacttc ggagaaaagg
      361 catttacaca gcatcctaaa caggttcagc ataggtgata taccgccttt tcattttctt
      421 ctccattatt tataagggag gtgtattatt atgcaatgtt attggtttta tccattgttt
      481 tttagtttat gaatgttcgc aaaataaatg aacaataatt ttatgtattt aaaataaatt
      541 aattttactt ctgtaatggt gtcatgatta gcagcttttt aaactaatat cgactttcaa
      601 taaataagga caggtaatgg acaacaaaaa tatctgaacc cgggtgagat tcataagttg
      661 cacagtgctg tcttcaaagt gccacacccg gaacgtaatc actgcctgct ccttatgggg
      721 tatcttcatg gtgtacaagc atcagagtta ttaggtataa aactgtctga tattgatttg
      781 caggccggta acctgaatat ccggcggctc taaacggttt gtcaactcag cccctcggct
      841 gaaacaggag gtacagtcca ttcgtcgctg gctgaaactt cgggatgaat gggtagaaga
      901 gggatatgaa tggttattta acctctatgg caggtctgct acaggtatta ctgttgttct
      961 ggggggcact ggtgtggtta atatctacga tgcaagagat tctcacgatg gcccggtcca
     1021 tcagtgcatg cggcgcgata ttgtgcgagt atttttcagg gggagtggta tccggatgca
     1081 gaaaattccg ctactcgact ggttttatgc aataaatcag gatgataatc ttctgatttt
     1141 ttactgaggc ggatatctct cgctgtgttt actgaaattg cggtattctg ttttttctga
     1201 aaaacagaaa atctgattaa aaagagcata tgcgttaagg gaaatttaag ttctggttga
     1261 ttaactgata aaaatattaa aaaatccctg ttgtcggtaa tctccggacc attccgggat
     1321 tacacaataa tatcaccatg tgtgatatct atggatcctc actgttgttt acgcgaggat
     1381 attttcagaa tgcttaacag acgataattt tttatgttga atgcaaggca tcttcccgta
     1441 ctgatgtatc atcatgtcag tcgttgtccg gggcttgtga cactctcacc tgaaacattt
     1501 tgtgaacaga tgaagtggct cgcagataat aactggcgga cggtaacatc tgctgagctt
     1561 gaatattttt atcagggggg aacgcttcca cgaaagagtg tcatgctcac attcgatgat
     1621 ggttatcttg ataactggct cagagcatgg ccggtcctga aggaatataa ccttcatgca
     1681 catatttttc tcattacggg gctgattggt aagggaaacg tacgtagcag gcaggagcag
     1741 gaatattctc accgcgactg tgaacggttg attgccgata accgttcaga tgaggtcatg
     1801 cttcgctggt ctgaagtccg ggaaatgcgt gattccggac tggttgaatt tcatgtgcac
     1861 acccattcac ataaacgctg ggacaggttg tctgtatcac gtgctgaaca gtgccggctc
     1921 atgaaagaag atattctggt gggaaaacaa tgtctgacgg aaaagctggg gttctgtagt
     1981 tcccatctgt gctggcctga aggatattat aatcgggatt atattaatct cgccggtaag
     2041 cttggtttct cttatttata tacaaccgaa agaagaatga actgtcctga aaatggttca
     2101 ttacgcatag ggcgtatcag caccaaagag cgggaacatt ccggctggct gaaacgacgc
     2161 cttttttatt atacaacgcc acttttttct tcagtactgg ctcttcataa ggggccaaga
     2221 ttaccagata attaatgatg aaaattttat tcactgaatc ttcgtcagat attgggggcc
     2281 aggagctgca ggcactggcc cagatgactg ctcttcaaaa gcagggacat tccgttttac
     2341 ttgcctgcag ggaaaaaagt aaaattgctc cggaagccag aaaaagaggg catgatgtca
     2401 cttttattcc tttcaggaac agccttcacc ttccttccat cctcagactt cgacggatta
     2461 tcggggaatt taaacctgat ctggtcatct gccatagtgg ccatgacagt aatatagcag
     2521 gactgagccg tctgatatgc tgtcaccgct tcagtattgt cagacaaaaa acctacatca
     2581 cccgtaagac ccgaacgttt tccctgaatt atctgtgtga tttcattgtt gtgcctagct
     2641 cagccatgat ggcgcatctg atggcggaag gtgtcaggac accggtaacg gttattccac
     2701 cgggtttcga ctggcccgca ttgcataatg aagccatgcg accgttaccc ctgcatatcc
     2761 acgcctgggc tgcatcagca gacaatgttc cgcttatcgt tcaggtgggg atgctacgtc
     2821 ccgaaaaagg acatgaattt atgctgcgtg tattgtatca gctgaagatg gaagggaaat
     2881 catttcgctg gctcgtggtg ggcgccgggc gtgaagaata cgaagcacgt ctgcgtcagc
     2941 agacagaaca cctgggaatg agcggagatg tgttaatggc aggtgccctt ttcccagcgt
     3001 taccggtcta ccggattgcc agtgtggtgg tcatgccttc ggaaaatgaa gcgtttggta
     3061 tggtactggc tgaagcgtca gtctctggcg tgcccgttat cgccagtgag acgggcggga
     3121 tccctgatgt cattcagaag aatgtgacag ggacgctgtt acccgtgggg gatgtgtctg
     3181 catggaccgg cgcactgcgc gattttttat cccgaccgga acgcttccgg atgatggcag
     3241 ccagcgccag agaagatatt gagtaccggt ttgatattaa cagaacggca cagatcattg
     3301 tgtctctggc tagtcaggct aaggggaaat gtaacagata acggcggatc ctcttttcag
     3361 cccgaatttt gttcagcggt tttccggcaa gtaacggagt aatttatcac attgtttaaa
     3421 tacggggacg gtcaatgcat ttaaataccg gacagaacag gcctactttc agctggagtg
     3481 cgctgggatg ggcaattttt tattttggct ttttttccac tctcctgcag gtcatcatct
     3541 tcagcagtgg gtacagcgga acgaatggaa tacgggactc actgttattc agttgtctgt
     3601 ggttgatccc ggtgtttctc tatcctgatc ggataaaaat aattgcagct gttgtcggtt
     3661 tcattctctg gggcacgtcg ctggcagcac tttgttatta ttttctctat ggtcatgaat
     3721 tctctcaaag tgttcttttc gttatgtttg aaacgaatgc cagagaggct ggtgaatatt
     3781 tcagccagta tttcagcctt aaactgttgc ttatctcgct ggtatatact gcggtgtccg
     3841 tttttctgtg gacacgtctg cgtcctgtat atattccttt gccatggcgg agaattgtct
     3901 ctttcctgct gctttatgct ctgcttctgc atccggttgt tctgaaatcg ttaatcagac
     3961 aggagccgct gaatgatact ctaggcaaac tggcatcccg aatggagcct gctgccccct
     4021 ggcagtttgt atccagttat tatcagtacc atcagcaact gaatgcactg acaaccttcc
     4081 tgaatgaaaa tagcgcactg ccaccactgg gtaatctcag ggatgaatca ggggagagac
     4141 cacgcacact ggtcctggtg attggtgagt cgacacagcg cgaacgcatg agcttatacg
     4201 ggtatctacg tgaaacgacg ccggagctgg atgcactgcg taaaaccgat ccgggtctta
     4261 ctgtgtttaa taatgtggtg gcatcgcgtc cgtacaccat tgaagcattg caacaggccc
     4321 ttactttcgc caacgaaaag aatcctgatc tgtatctgac gcagccgtcg ctgatgaaca
     4381 tgatgaagca ggcaggctat aaaacattct ggattaccaa ccagcagaca atcacagccc
     4441 gtaacaccat gctcactgta ttttcgcgcc agacggacag gcagtactac atgaatcagc
     4501 agcgaacaca aagtgcgcgt gaatatgaca ctaacgtgct gaagccgttc cgggaagtgc
     4561 tgaatgaccc tgcaccaaag aaactgatca tcgtgcatct gctgggtacg cacattaagt
     4621 ataaataccg ttatccggaa ggtcaggggc ggtttgatgg cattacaggg catattccca
     4681 ctggattaaa tgcgaaagag ctggaagtgt ataacgatta cgataatgcc aatctgttta
     4741 acgatcatgt ggtggccagt ctgataaagg acttcagagc aactgcgccg gacggctttc
     4801 tgctttattt ttcagatcat ggtgaagaag tatatgatac tccgccatat aaaacgcagg
     4861 gacggaatga agataacccc acacgtccca tgtacactgt tccgttcctg ctgtggacct
     4921 cggaaaagtg gcatgctgca catccgcgag atttttcgca gtatgttgac cgcaaataca
     4981 gtctggctga actgatccac acctggtcag atttggcggg actgacatac gatggttacg
     5041 atccgacccg ttctctggtg aatccgcaat tcagggaaac tacccgctgg attggaaatc
     5101 cgtataaaaa gaatgggctc actgatttcg acactcttcc gtatggtgag ccatagtatt
     5161 ttcttatctt cggttccgtt ctgaaggcag gaggatgtgt tttcctctgc tgtccggtgg
     5221 actccaggga tggtaccacc ttctgctgta ctctgttatc ataatgagat aagcaggcag
     5281 ataccggtaa atatgaaaaa tatcagaact gagtttattc cccgctttaa ccttacatta
     5341 tgcttccccc gttactggat gacgtggaca ggtatcggta ttatctgtgt gtttgcaatg
     5401 gtcccccccg cactgagaga tccattgttg ggaaaattgg gcatgctggt tgggcgtctg
     5461 gggaaaagcg cacgtcaacg ggccttgatt aatttatcgc tttgttttcc ggagtacagt
     5521 gataaagaaa aggagaatat cgttgatgcc atgtttgcaa cagcctccat ggccgttgtg
     5581 cttatggctg aattagcact gtccgggcct gataaaattt cgcatcgtat acgctggaat
     5641 ggtctggaga ttgtggagaa gatggcacag aacaatgaaa aggtcatttt tcttgtgcca
     5701 catgcctggg gagtggatat tcccgcaatg ctgatagctg cgtcgggcag aaaaatggca
     5761 gcgatgtttc ataaccagag aaatccggtg gtggattatg tctggaacag tgttcgtcgt
     5821 cgtttcggag gaaaactgca cgcacgtaat gacggtattg cctcctttgt ccgctcagtc
     5881 cggcagggat actggggata ttatcttcct gatcaggatc atggcccgga attcagtgag
     5941 tttgcagatt tttttgcgac gtataaagca actttaccgg ttatcggacg gctcagcagg
     6001 atcagtggtg cacggataat accgcttttc cctgtttatg acggaaagac ccatcatctg
     6061 actattcatg tcagcccgcc acttgctata cgacagaaga gtgatgctca tattgcgcga
     6121 cagataaacg aagtggttga aaatttcgtt cgtccacatc ctgagcagta tacgtggatc
     6181 ctcaagttgc tgaaaacgcg taaagaaggg gaagaggatc cttactgagg caggtttttc
     6241 aggataatga gcttacccag caatagtgga cacacgctaa gtgagtaaac tctcagtcag
     6301 aggtggctca tatgacaaaa aaacagtacc aaccagtaaa aaaccccgta aatagcattc
     6361 gcctgaattt cgcagtgaag ccctgaagct tgctgaacgc atcggtgttg ctgtcgcagc
     6421 ccgtgtgctc agtctgtatg aatcacagct tgtaagcggc tcgtcagaac tgtattgata
     6481 tttactgagc ggtcgtaagc gtacagcgag ggccgtattg acggggatgt gttattcagc
     6541 tggcagtgct atgcgccacg gaagcagttc gctgacccgg ttgaccggcc agtctgctat
     6601 gacgccaggc acatggcgaa ggtagctttc tggatccacg tcattcagtt tgcacgtccc
     6661 gatcaggctg tacagtagcg ctcc
//
LOCUS       DAZFRJ010000222         6648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000222 DAZFRJ010000000
VERSION     DAZFRJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.224"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..340)
                     /locus_tag="ACHKUN_004161"
     CDS             complement(38..340)
                     /locus_tag="ACHKUN_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6662309.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            438..809
                     /locus_tag="ACHKUN_004162"
     CDS             438..809
                     /locus_tag="ACHKUN_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662310.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            830..1021
                     /locus_tag="ACHKUN_004163"
     CDS             830..1021
                     /locus_tag="ACHKUN_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO6662311.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            1023..1301
                     /locus_tag="ACHKUN_004164"
     CDS             1023..1301
                     /locus_tag="ACHKUN_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6662312.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            1588..1740
                     /locus_tag="ACHKUN_004165"
     CDS             1588..1740
                     /locus_tag="ACHKUN_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6662313.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
     gene            2161..2382
                     /gene="kilR"
                     /locus_tag="ACHKUN_004166"
     CDS             2161..2382
                     /gene="kilR"
                     /locus_tag="ACHKUN_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO6662314.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            2382..2552
                     /gene="ydaE"
                     /locus_tag="ACHKUN_004167"
     CDS             2382..2552
                     /gene="ydaE"
                     /locus_tag="ACHKUN_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO6662315.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            2627..2902
                     /gene="racC"
                     /locus_tag="ACHKUN_004168"
     CDS             2627..2902
                     /gene="racC"
                     /locus_tag="ACHKUN_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HFO6662316.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            3004..5604
                     /gene="recE"
                     /locus_tag="ACHKUN_004169"
     CDS             3004..5604
                     /gene="recE"
                     /locus_tag="ACHKUN_004169"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO6662317.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDYEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYLETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            5597..6406
                     /gene="recT"
                     /locus_tag="ACHKUN_004170"
     CDS             5597..6406
                     /gene="recT"
                     /locus_tag="ACHKUN_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO6662318.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            6462..6611
                     /gene="ralR"
                     /locus_tag="ACHKUN_004171"
     CDS             6462..6611
                     /gene="ralR"
                     /locus_tag="ACHKUN_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO6662319.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
BASE COUNT         2016 a         1504 c         1571 g         1557 t
ORIGIN      
        1 acattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatcaact ccatcagata
       61 agcatgcact acaatcacct tcagcatgaa ctacatgtgt ttgccccaaa cgaatgccgc
      121 tcgcatactc aggccaaata agctcccaat catgaggtcg tagctccgcc ctacttactt
      181 ggccttccgt cgcagattcg atcataaggg cgcgggttgg agatatagct gttcgtccag
      241 acgccatttg cgataagtaa gatggcgata caccaagtct ggccgcgaat ttcttagcat
      301 caccaaccct taatgattta ataaactctt ttaatgtcat accttcctcg gtttagtgtt
      361 tttttgcaag tttagtgttt aataaaccat taagtcaagt atttgcttgt ttagtgatta
      421 ctaaagataa ttaccacatg caaaaaaaag aaattcgccg tttacgtctc aaggagtggt
      481 ttaaagataa aactctgcca cccaaagaga agagctacct atctcaacta atgagtggga
      541 gagcctcgtt tggagaaaag gctgccagaa gaatagagca aacatacggg atgccggaag
      601 ggtatctgga tgcggaatac gcagaacaac cgggggtttc tccaccacat gcagggttaa
      661 cgtctaatca actggaatta ttgcagattt tttcagcctt ccctgaggat gagcaacgcc
      721 agataatcag cgagttaaag cagaaaaaag aatcaatgga agatctcata gcgagatgga
      781 ttgcggcgca aaaatgccgc cgcgcctgag ttataaaacc ggaggaaaca tgaatagagc
      841 cctttcacca atggtttctg aatttgaaac cattgaacaa gaaaacagtt acaacgaatg
      901 gctgcgtgcg aaagtagcaa cgagccttgc agatccgcgc ccagcaattc cccatgacga
      961 agttgagcgc agaatggcag aacgctttgc taagatgcgc aaggaacgga gcaagcagta
     1021 aaatgttacc cgtgttatgg cttgaaagcg cagataccga cctagatgat ataactagtt
     1081 atattgctcg tttcgacata gatgcggctg aacgcttatg gcagcgatta aggggttgtg
     1141 tgctgccgtt atccgaacat ccgtatttat acccaccaag cgacagagta cctggcttgc
     1201 gtgagattgt agcccaccct aactatataa ttctataccg cgtaacaaca tcaagcgttg
     1261 aagtagtaaa cgtgatccac gcaagacgcc agtttcccta actttcacta ccaatagaaa
     1321 cataacaacc gcaacgactt tatcaaaagc gttgtgtttg ttatgtcccg cggtttagtt
     1381 tttacttgac ttaagtttaa tgtttattaa actaaaaata ccaccccgcc ccacagaacg
     1441 cagggaaata cttcgagtta cccggcagtg gtcaggggtt aagtagccag cccgaggcgt
     1501 atgaacatga cggcagggtt caactttaat aactatgcag caggtttttg ttccgctacc
     1561 ccggcgttaa ggggaaatga ggtcagcatg gatactatcg atcttggcaa caacgcatct
     1621 ctggtgtacg gcgtgtttcc aaaccaggac ggcacgttca ccgcaatgac gtataccaaa
     1681 agcaaaacgt ttaaaaccga aaatggtgcc cgtcgctggc ttgccaaaaa taccagctaa
     1741 accatttatt ggattaattc aatattcttg ctgtaggggt atagccgagg ccaccaaagc
     1801 ccggaggtgg tgaaataaaa ccgggcacaa cacgaaggcg catttccggt atccataaag
     1861 agtcggtctt gtctgttaaa tttaaatggt gggagtgcgc ctccggttgt gaataacaac
     1921 actgctgtgt gtagtcttgg cggcatcagt tttttcttga agttcgactg atgtccgccc
     1981 tttttaaagt gaattttgtg atgcggtgaa tgcggctaag cgcacgcggc acagttaaaa
     2041 gtcatgttag tccttattgg tttgggtggg aaagccgact gtaattgtta actggttgca
     2101 gtcacctgga ggcaccaggc accgcatcaa caaagttcat ttgtaaaaat ggagataatt
     2161 atgattgcac atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
     2221 tatgttcttc atgaaggccg gacatatatt gcctcggcaa acaatattaa aaagcgaaaa
     2281 ctatatattc gtaacctgac cacaaaaaca tgcattactg accgcatgat taaagtcttc
     2341 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt gatgactaag aaaataaaat
     2401 gtgcttacca cctttgcaaa aaagacgttg aagaaagcaa agctattgaa agaatgcttc
     2461 acttcatgca cgggatttta tcaaaagacg aaccgagaaa atattgcagt gaagcttgtg
     2521 ccgaaaaaga ccagatggca catgaacttt aattaattga ctattcgaaa ctgaatttat
     2581 gccagaaatg gcaggtattc gctcaacctt aattaaggag aaaaacatga ttaccaatta
     2641 tgaagccact gttgtaacta ccgatgacat tgttcacgag gtgaatctgg aaggaaagcg
     2701 cattggctac gtaattaaaa cagaaaataa agaaacccca ttcactgtgg ttgatatcga
     2761 tggtccatca ggcaacgtaa aaacacttga tgaaggtgtc aaaaaaatgt gcctggtgca
     2821 tatcggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc taattgcaat
     2881 gaaattaaaa ggtgaaatct gaaagaaata gcctgcgtat ggcgcaggct atgaacagtg
     2941 tgtatccggc aagatcattc actgaacaaa acgaatttta atctgagttg aggttaaaaa
     3001 acaatgagca caaaaccact cttcctgtta cggaaagcga aaaaatcatc cggtgaacct
     3061 gacgtcgtcc tgtgggcaag caacgatttt gaatcgacct gtgccactct ggactacctg
     3121 atcgttaagt caggtaaaaa actgagcagc tattttaaag ctgttgccac gaattttcct
     3181 gtcgttaatg acctgcccgc tgaaggtgag atcgatttta cctggagtga acgctatcaa
     3241 ctcagcaaag actccatgac atgggaacta aaaccgggag cagcaccaga caacgctcac
     3301 tatcaaggca ataccaacgt caacggcgaa gacatgactg agattgagga gaatatgcta
     3361 ctcccaattt ctggccagga actgcccatt cgttggcttg ctcaacacgg cagcgaaaaa
     3421 ccggtaacgc acgtttcacg cgacggactc caggcattac acattgctcg ggctgaagaa
     3481 ctaccggctg ttactgccct ggctgtttcc cacaaaacca gcctgctcga cccgctggaa
     3541 attcgcgaac tccacaaact ggttcgtgac actgacaaag ttttccctaa tcctggtaat
     3601 tcaaacctgg gactgataac tgcttttttc gaagcatacc tgaacgctga ctacaccgat
     3661 cgaggactgc tgacaaaaga gtggatgaag ggtaatcgtg tttcacacat cactcgcacg
     3721 gcttccggtg ctaatgctgg cggcggaaac ctcaccgatc gcggcgaagg tttcgtacac
     3781 gatctgacgt cactggcgcg ggacgtagcc actggcgtac tggcccgttc aatggatctg
     3841 gacatctata accttcatcc ggcacacgct aaacgcattg aggaaattat cgctgaaaat
     3901 aaaccgccct tttctgtttt ccgcgacaaa ttcatcacca tgcctggcgg gctggattat
     3961 tcccgcgcca tcgtggttgc gtccgtaaaa gaagcaccaa ttgggatcga ggtcatcccc
     4021 gcgcacgtca ctgaatatct gaacaaagta ctgactgaaa ccgatcatgc caaccctgat
     4081 ccggaaatcg tggatattgc ctgcggtcgc tcctctgccc cgatgccgca gcgagtaaca
     4141 gaagaaggaa aacaggatta tgaagaaaaa ccgcaaccat ctggaacaac ggcagttgaa
     4201 cagggagagg ctgaaacaat ggaaccggac gcaactgaac atcatcagga cacgcagccg
     4261 ctggatgctc agtcacaggt aaattctgtt gatgcgaaat atcaggaact gcgggcagaa
     4321 ctccatgaag cccggaaaaa cattccatca aaaaatcctg tcgatgccga taaattgctt
     4381 gctgcatcac gtggtgaatt tgttgacgga attagcgacc cgaacgatcc gaaatgggta
     4441 aaggggatcc agactcgcga ttgtgtgtac cagaaccagc cagaaacgga aaaaaccagc
     4501 ccggatatga atcaacctga gccagtagtg caacaggaac cggaaatagc ctgcaatgcc
     4561 tgcggccaga ctggcgggga taactgccct gactgtggtg cggtgatggg cgacgcaaca
     4621 tacctggaaa cattcgatga agagagtcag gttgaagcta aggaaaatga tccggaggaa
     4681 atggaaggcg ctgaacatcc gcacaatgag aatgctggca gcgatccgca tcgcgattgc
     4741 agtgatgaaa ctggcgaagt cgcagatccc gtaatcgtag aagacataga gccaggtatt
     4801 tattacggaa tttcgaatga gaattaccac gcgggtcccg gtgtcagtaa gtctcaactc
     4861 gatgacattg ctgatactcc ggcactgtat ttgtggcgta aaaatgcccc cgtggacacc
     4921 acaaagacaa aaacgctcga tttaggaacc gctttccact gccgggtact tgagccggaa
     4981 gaattcagta accgctttat cgtagcacct gaatttaacc gccggacaaa ctccggaaaa
     5041 gaagaagaga aagcgtttct gagggaatgc gcaagcacag gaaaaacggt tatcactgcc
     5101 gaagaaggcc ggaaaattga actcatgtat cagagcgtta tggctttgcc gctggggcaa
     5161 tggcttgttg aaagcgccgg acacgctgaa tcatcaattt actgggaaga tcctgaaaca
     5221 gcaattttgt gtcggtgccg tccggacaaa attatccctg aatttcactg gatcatggac
     5281 gtgaaaacta cggcggatat tcaacgattc aaaaccgctt attacgacta ccgctatcac
     5341 gttcaggatg cattctacag tgacggttat gaagcacagt ttggagtgca gccaactttc
     5401 gtttttctgg ttgccagcac aactattgaa tgcggacgtt atccggttga aattttcatg
     5461 atgggcgaag aagcaaaact ggcaggtcag ctggaatatc accgcaatct gcgaaccctg
     5521 gctgactgcc tcaataccga tgaatggcca gctattaaga cgttatcact gccccgctgg
     5581 gctaaggaat atgcaaatga ctaagcaacc accaatcgca aaagccgatc tgcaaaaaac
     5641 tcagggaaac cgtgcaccag cagcaattaa aaataacgac gtgattagtt ttattaacca
     5701 gccatcaatg aaagagcaac tggcagcagc tcttccacgc catatgacgg ctgaacgtat
     5761 gatccgtatc gccaccacag aaattcgtaa agttccggcg ttaggaaact gtgacactat
     5821 gagttttgtc agtgcaatcg tacagtgttc acagctcgga cttgagcccg gtagcgccct
     5881 cggtcatgca tatttactgc cttttggtaa taaaaacgaa aagagcggta aaaaaaacgt
     5941 tcagctaatc attggctatc gcggcatgat tgatctggct cgccgttcag gtcaaatcgc
     6001 cagcctgtca gcccgtgttg tccgtgaagg tgacgagttt aatttcgaat ttggccttga
     6061 tgaaaagtta atacaccgcc caggagaaaa cgaagatgcc cctgttaccc acgtctatgc
     6121 tgtcgcaaga ctgaaagacg gaggtactca gtttgaagtt atgacgcgca aacagattga
     6181 gctggtgcgc agccagagta aagctggtaa taacgggccg tgggtaactc actgggaaga
     6241 aatggcaaag aaaacggcta ttcgtcgcct gttcaaatat ctgcccgtat caattgagat
     6301 ccagcgtgca gtatcaatgg atgaaaagga accactgaca atcgatcctg cagattcctc
     6361 tgtattaacc ggggaataca gtgtaatcga taattcagag gaataattca gcctggcggt
     6421 gtaatgcacc gccaacttga aatattttta tgagaaaaat tatgagatat gacaatgtta
     6481 aaccatgtcc attttgtggt tgtccatcag taacggtgaa agccatttca ggatattacc
     6541 gagcgaagtg taacggatgc gaatcccgaa ccggttatgg tggaagtgaa aaagaagcac
     6601 tcgaacgatg aaataaacga accactggaa ataataatgg aggtgttc
//
LOCUS       DAZFRJ010000223         6562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000223 DAZFRJ010000000
VERSION     DAZFRJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.225"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..5733
                     /gene="efa1"
                     /locus_tag="ACHKUN_004172"
     CDS             <1..5733
                     /gene="efa1"
                     /locus_tag="ACHKUN_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO6662320.1"
                     /translation="LNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQ
                     HLTGLPTIADATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFN
                     DNIISSAPVVSSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLL
                     VTQEGRCMGLALLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRT
                     YSLIEMLQYQGNKYITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLE
                     DIYRLTDPNFGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESD
                     MVWNKLLPENDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRIT
                     SPPTSEQIPSLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRG
                     TQDDLVSSQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSG
                     RFNLQIRDGETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYAR
                     LLQQGHEDSTLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGM
                     RSVATRTGGTMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQ
                     IAFDSISLGLTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFL
                     TDGSKHIVVASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLG
                     DWQIREKVGYANSISPYSSLAHGYANSKWPQTIPKIPSGEYDTIILGYGHQYQANTEI
                     EYLSNWIVWREAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTE
                     QAISLKDYKFILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLAFDLS
                     KQTQSVMLKTTDGEVPVMTITQKGINTLVGTTAGENWLTGNDKDNTFHTSSGGGTVIS
                     GGGNNRYIIPRDLKTPLTLTLSNSSVSHEILLPETTLTELKPVAFELSLIYWAGNNIK
                     VQLEDETQLNRFAGNFRGHTSDGITLEAVSQENGIQLGVSSCDVQRWQAVYPEENNRP
                     DAILDRLHDMGWSLAPEVRFRGGETSASYDPLNRQLVYRLQERYSEFRLTGSRHYTTA
                     VTGTPGSRYIITGPDTAQISPAQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILT
                     IATIQYSLQLTISGIDESLPGTTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTP
                     TVNRLENLMALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEG
                     EPTVPVNISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQ
                     EHIIVKPDNEAPSLILSEFRRFTISSDKKFSLKLMCHQGMVRIDRRSLSVRLFYLREQ
                     PGIGSLRLTFRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGV
                     LNLADIVAEYARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGEPFPT
                     FHPWYTDDLSGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRH
                     EWEKYNLSILSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFIN
                     GEQMLSYDSHSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPE
                     LLRHTPGYIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYY
                     RIDGMAGADIADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQS
                     KTPNGDNLFN"
     gene            5905..6255
                     /locus_tag="ACHKUN_004173"
                     /pseudo
     CDS             5905..6255
                     /locus_tag="ACHKUN_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1927 a         1508 c         1515 g         1612 t
ORIGIN      
        1 ctaaattcac agaatcttcg tcagcttctg gaaaactctg tttcttcatg ctttttgact
       61 gagcaaagta ttaataaatt acttagtgag gcagaaaaaa ggccttatca gtctttaaca
      121 gaaatacatc agcatctcac aggattacca actattgccg atgcaaccct ttcattactt
      181 tctgttggat tacctggtac gggaaaacta ttgcgcaggg agcaggacta tgggcgtcca
      241 ccagttacag caattcagga ttccacattt gtactccctt ataatttcaa aggtattggt
      301 tttaacgata acattatatc ctctgcacct gtagtctcct cattacattt tatcgctgaa
      361 catgcgaaat atactttgtt gtcatggcct gagttttatc gtcatcatgc acagcgatgg
      421 ttcgaaatgg ctaaaggata tggaagccag aatattgatt ttcaccctca gtctctattg
      481 gtaacccaag aaggacgctg tatgggatta gccttacttt atttacagac tgaagatact
      541 gctcattata gcattctcca ggaaaaccta atgactgtga gtgcacttca tcagaccagt
      601 aatcgcgata agttgccact gtccaaagat gataattcct taatgacaag aacttatagt
      661 ctgattgaaa tgctacagta tcagggaaac aaatatatta ccaacgaatc gctactacat
      721 aagaccgcat ggaaccaaga aagaataact ttattattca atgaaaaagg agttaagcga
      781 gccctgataa gcacgcctaa tcatactctg gttctgcaac aactggagga tatttaccgg
      841 ctcactgatc caaattttgg gcatgcagat ttcctttcac ctatagatgc tctgaagttt
      901 attgaggcta tgatacaatt aactccaaca cttcaggaat attatggcct attaaacaaa
      961 gacattaata aacatataca agtacattat gccgaatcag atatggtctg gaataagctt
     1021 ctgccagaaa atgatgctgg actgagcacc agaattcagc acaccaccac cgaccgtctg
     1081 gcgaatctgg ctgaaccagt cgctgttgca ggtatctccc tgccagtaaa aacactttat
     1141 gatatcggag ccacccttga cggtcggcgc atcacctctc ctccaacatc ggagcaaatc
     1201 ccttctctgc gtctcaacgg tgatgttctg aatgattatc tgtcccgcac agttctgact
     1261 ccagaacagg ctgataacat aagaaaaata ctgcacactc agggaatacg cagcggtacc
     1321 cgtcccatag atccggagat gattcgtggg acgcaggatg acctagtttc gtcacagact
     1381 cgtctgcaaa ggcaagcaac acgggttaaa cagcaactcg ccggcgtact tgatactctg
     1441 caacagcact tccagaacat tccacgttca tccggtcgtc atctttctgt agagaatatt
     1501 gagctggctg atatcggaag tgggcgtttc aaccttcaaa ttcgagatgg agaaacattg
     1561 cataccactt ctgtggaagt accggaagtt gtgtcccgtt ttcagaaact ttctaccatg
     1621 ctttcagccc tgcctgccag tggaatcatg gatttcgacc tcggcatgag tgtggtcgga
     1681 gtcgtccagt atgcccgcct gctacagcag gggcacgaag acagtaccct agccaagata
     1741 aatctagcta tggatatcaa gcaactttcc gaagcaactc tcggcagcat gattcagatt
     1801 gccgggaata agtttctcaa tacagaagga atccaggggt tcagactgga aagcgccgtc
     1861 gctgaaggta tgcgctcagt agcaacccgt accggaggca caatggggaa agccctttct
     1921 gccagtgccc gtgttcttga actgcctgta ctggaaacag ttctggggac atggaacctg
     1981 tacaacagcg tcattcagct ccagcaagct acatcttatt ctgagacaat ggctgcccgg
     2041 gtacagattg cgtttgattc catttctctg ggattaactg ccgcttcggt agctttcccg
     2101 ccactaatta ttgccactgg ccccattgcg gctattggta tgggagcttc cagtattgca
     2161 cgtaatgtgg cacggaaaga agaacggcat acacaatggc tggaatataa aaaattcctg
     2221 actgatggca gtaaacacat tgttgtggcc tctccggaaa gaggtctgct ggatttctcc
     2281 ggaaacaaag tttttggaaa aatggtgctg gatctgcgtc agtctcctcc tctcttgcat
     2341 ggagaaagct cttttaacgc tgaccgcaaa atcggtcatc gtccggatct gggggactgg
     2401 caaattcgtg agaaggtggg gtatgccaac agtatcagtc cctactcttc cctggcgcac
     2461 ggttacgcca acagtaaatg gccacaaaca ataccgaaaa ttccctcggg agaatatgac
     2521 accataattc tgggctacgg tcaccagtat caggccaata cggaaataga atatctgtct
     2581 aactggattg tatggcggga agccgtacca gacagtactt cccgccacaa acgtcctcct
     2641 ctggaggttc ttaatagtca gtgtactgtg atagctggag agcgtaaaac cacagtactt
     2701 cccctgagag tgctcagcga tctgacaccg gaatgcacag aacaggctat atcgttaaaa
     2761 gattataaat tcatactgag agggggaagc ggtgggctgg ctgttcaggt cggtggcgcg
     2821 ggatattatg atattgatgc aaatcctgtg gcaaaagaaa atacgctctc ttttcgcggg
     2881 ctaccggaag agtttccgct cgcctttgat ttatcaaaac aaacacagtc ggtcatgctg
     2941 aaaacaacgg acggtgaggt gccggtaatg accatcaccc agaagggaat aaacaccctg
     3001 gtcggtacaa cggccggcga aaactggcta acgggtaacg ataaggacaa taccttccat
     3061 acgagttctg gtggcggtac tgtcatctct ggtggaggga ataaccgcta tattatcccc
     3121 cgcgatttaa aaactccgct gacactgacg ctgtctaata gctcggtctc tcacgagatc
     3181 cttctgccag aaacaactct gacagaatta aaacctgtcg cctttgagct gagtctgatt
     3241 tactgggccg ggaacaatat aaaggttcag ctggaggatg agacacaact gaaccgtttt
     3301 gccggaaact tcagggggca taccagtgat ggcattactc tggaggcggt ttcccaggaa
     3361 aatggtattc aactgggggt ttcatcatgt gatgttcaac gctggcaggc tgtttatccg
     3421 gaagaaaaca acagaccaga cgccatactg gacaggctgc atgatatggg atggagcctg
     3481 gcccccgagg tcaggttccg gggaggagaa acatctgcca gctatgaccc cctgaaccgt
     3541 cagctcgttt accggcttca ggagcgttac tcggaattca ggctgaccgg cagtcgccat
     3601 tacaccacgg ctgtaaccgg aacccccgga agccggtaca ttattacggg gccggataca
     3661 gcacagatat caccggcaca aatcatactg gccggtgata atgaccatcc ggaaacgatt
     3721 gatttgcttg aggccagtcc tgttctggtt gaagggaaaa aagacaaaaa cagcgtgata
     3781 ttaacgattg ctacgattca gtattccctt caactgacaa tatccgggat cgacgaatcg
     3841 ctgcccggga caacccgtgt ggcaattcag cctcaggata cccgtttact gggtgacgta
     3901 ctccggatct taccagataa tggtaactgg gtggggattt tccggagtgg tcatacacca
     3961 acggtaaacc ggctggaaaa tttgatggca ctgaatcagg taatgacgtt cctgccccgg
     4021 gtatccggaa gtgcagagca ggtattatgc ctcgaaaacc taggtggcgt aaggaaaaaa
     4081 gtggaggggg agttactgtc agggaagctg aaaggtgcgt ggaaagccga aggtgaacct
     4141 actgttccgg taaatatctc agatctaagt atcccaccct attcacgtct gtatctgatt
     4201 tttgaaggga aaaataatgt gttgctacgc agtaaagtac atgcagctcc gttgaaaata
     4261 acatccgcag gagagatgca gttatctgaa aggcagtggc aacagcagga acatattatt
     4321 gtcaagcccg acaacgaagc cccctcatta atactcagtg aatttcgtcg tttcactatt
     4381 tcatcggata aaaaattttc tttaaaactg atgtgccatc aggggatggt ccgcatcgac
     4441 cgcagatcat tatcggtcag attgttctat ctgcgtgaac agccaggtat cggcagttta
     4501 cgtctgacgt tcagagattt tttcacagaa gtgatggata caacggacag ggaaattctg
     4561 gagaaagagc tgagaccaat tctgatagga gatacacacc gctttatcaa cgctgcatac
     4621 aaaaatcatc tgaatatcca gttaggagat ggcgttctga atctggcaga cattgttgcg
     4681 gaatacgccc gtattcaaaa ggaagaaaca tcaaaaatat tgtatcaata tcaaggtgcc
     4741 atgaaaaaaa aaacagatgg accatctgtg gtagaagatg ccattatgac cactactgtc
     4801 acaacagatt caggtgaacc attccctacc ttccacccgt ggtatacaga tgatttatca
     4861 gggcgttata agagcgtacc tatggcaaga aaagcagata ctttgtatca cctgacacca
     4921 aaaggtgatc tacagataat atatcaggta gctacaaaaa tggtgaatca ggcgatgatt
     4981 gtatccctgc caaactaccg acacgagtgg gaaaaatata atttaagcat cttatccgaa
     5041 atccctcaga acaataatac tgttgtacat tcaatcctca gggttaatgg ccccacaatg
     5101 caggtgcgca caattgacta cagaggaacg gatgaaaaca atcccatagt atctttttca
     5161 gatacaacct tcatcaatgg tgaacagatg ttgagttatg actcgcattc atcagggcga
     5221 gtctattcca gagaagaata tatgatgtgg gaattgcagc aacgggtatc agaagcttcc
     5281 agtgcccgga cacaggatta ctggctgatg gatgcagcgg taagaaacgg agaatggaag
     5341 atcacaccag aattattacg tcacacaccg ggatatatcc ggagtacggt atcgaaatgg
     5401 tccagaggat ggctgaaaac cggcacaata ctccagactc cagaagacag aaatacggat
     5461 gtatacctga ctaccataca gaacaatgta tttagtcgtc aggggggcgg ctaccaagtg
     5521 tattatcgga ttgatggaat ggctggtgcg gatatagcgg ataatgcacc aggggaaacc
     5581 cgctgcaccc tcaggcccgg aacatgtttt gaagtgacaa gtgtggatga aaggcattat
     5641 gagtggaata tcatttatgt cacgctgaaa acctgtggct ggagccgaaa tggccaaagc
     5701 aaaacgccga atggtgacaa cctttttaac taattaaaat ctctgttaaa gatgtataac
     5761 ccggcatcta tgtgctcacc agcatataga tgcaatgtga ttcccctgat ggtcgtatca
     5821 gagatggtat gcgagcctgt acatagattt gtgtaattgc ctgattttga tatgttcaat
     5881 ccaacatcaa aagcaggtta atttatggac gaaaaacagt tgcaggctct ggctaacgaa
     5941 caggccaaaa atctcaaaac ccctgaagat ctcagccagt tcgatcgcct gctgaagaaa
     6001 atcagcgttg aggcagctct caacgctgaa atgtcccacc atctgggcta cgataaaaac
     6061 cagcctaaac cgggtgccaa ctcccgcaat ggctattcca caaagaccgt tatcaccggt
     6121 gatggccccc tggaactacg cactccgcgc gatcgtgatg gctcttttga accgtaactt
     6181 gtgaagaaaa accagacccg gatcaccggg atggataacc agatcctgtc gttgtacgcc
     6241 aaaggcctga caaccgtaag cgtacagcct gaaccgtctg gtcagaatct gacgaattag
     6301 agtaagctgg acatccccag atatattatg gctgccagcg tcatatcggc ataaatagag
     6361 atatccctta acgctaacca gaaaaaaata cctataagag cctgtacata gagttgtgtc
     6421 gttgcctgat gttgatatgt caaatccaac atcaaatagc ggtaaattta tggataaaaa
     6481 attacaggct ctggttaacg aaacaccccc ctcttgctct attcccctct ttcatcattt
     6541 attacaatat ttatttcaat aa
//
LOCUS       DAZFRJ010000224         6490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000224 DAZFRJ010000000
VERSION     DAZFRJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.226"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            131..694
                     /gene="fimA"
                     /locus_tag="ACHKUN_004174"
     CDS             131..694
                     /gene="fimA"
                     /locus_tag="ACHKUN_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6662321.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            1055..1765
                     /locus_tag="ACHKUN_004175"
     CDS             1055..1765
                     /locus_tag="ACHKUN_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6662322.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1807..4458
                     /locus_tag="ACHKUN_004176"
     CDS             1807..4458
                     /locus_tag="ACHKUN_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6662323.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            4472..5002
                     /locus_tag="ACHKUN_004177"
     CDS             4472..5002
                     /locus_tag="ACHKUN_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6662324.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            5015..5518
                     /locus_tag="ACHKUN_004178"
     CDS             5015..5518
                     /locus_tag="ACHKUN_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6662325.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5577..>6490
                     /locus_tag="ACHKUN_004179"
     CDS             5577..>6490
                     /locus_tag="ACHKUN_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6662326.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1869 a         1387 c         1444 g         1790 t
ORIGIN      
        1 taataaccct ggaaactata ttctcagggc aacaaccaat caagaatgat tcttgttttt
       61 aagcaataac agaatgactt aattgttaac cctctttatt cagctactta aatataaatt
      121 ttggagaatt atgaaactca aacatgttgg tatgattgtc gtttctgtgt tggcgatgtc
      181 gtctgctgcg gtaagcgcag ccgagggtga tgaatcagta acgaccactg ttaatggcgg
      241 tgttattcat tttaaaggtg aagtggtaaa tgccgcttgt gcaattgatt cagaatcaat
      301 gaaccaaacg gttgagctgg gtcaggttcg ttcttctcgc ttggctaaag cgggtgacct
      361 cagctccgcc gttggcttca atatcaagct gaatgattgt gataccaatg tttccagtaa
      421 tgcagctgtt gcattcctgg gtactactgt caccagtaat gacgatacgt tagcgctgca
      481 gagttcagcg gcaggctctg cccaaaatgt cggtattcaa attttggacc gtacgggtga
      541 ggtattaata cttgatgggg ccacttttag tgctaaaacc gacttgattg atggcacgaa
      601 tatactgcca ttccaggctc gttatattgc tctcgggcag tccgtagctg gtactgcaaa
      661 cgctgatgcg accttcaaag ttcaatatct ataattcaat cttaaggtgg ctatacgtca
      721 tctgaaattg aaacagggtg tgtgtggcat ggctacgcac acccgagcaa tttaccaata
      781 ttcatctcat ttttaatgag aaatggtttg tatttggagc gctatgaaaa atcacataat
      841 atctaaacta tcaataaaca acagtagtta aacttactgt tatttaaaat atttactttg
      901 tagcaaggtt ttcctattaa aagagaacca acatttatta caaacgcatt aagtctggaa
      961 acccggcgac tcgccaatgt attagcaaca ttaatcgatt gtctgatgta acggttttcc
     1021 cgcttatcct cttaacacat ggagaatgca tcagatgcaa actacgcgta ccccctactc
     1081 tatttccttt atggcgacag tgttactttt attgctcttt gcttgccata gcaccgttgc
     1141 caacgcagct gtcgcactgg gtgcgacgcg ggtaatttat ccggcaaacc agaaacaggt
     1201 tttattgcct gtcacgaata atgatcctgc cagtgtctat ctcattcagt catggataga
     1261 aaatgcaggt gaccaaaaag acacccagtt tgtcattacc ccaccgcttt tttccatgca
     1321 aggtaaaaaa gagaatacgc tgcggataat caacgctact aatcatcagt taccaggcga
     1381 ccgtgaaagc ctgttctggg tgaatgttaa agctattcct gctatggaaa aagaccagaa
     1441 gaatgagaat acattacagc tggcaattat tagccgcata aaaatgtttt atcgccccac
     1501 gaacctggca atggcacctg aagaagcgcc tgcaatgctt cggtttcgtc gcagcggtag
     1561 caaactcacg cttattaatc caacgcccta tttcattacc gtaacaaata tgaaagcagg
     1621 aaatagcaat ctcccaaaca ccatggtgcc gccaaaaggt gaagtatcgg tggatatcac
     1681 gcatgctgca accggggata tcagttttca gacgatcaat gattacggtg cattaactcc
     1741 gcgtattaag gcaaccatgc agtaagccag tgacctaatt aataataaac tcaatcggaa
     1801 ttaataatga cagcctttcg tgccgcattt aaggcatatc gtatgcatca ggtgttaata
     1861 atgcctcgct ttgcacggct taccattgcc cttggcttag caactgcggt atttcccgtt
     1921 gacgcagagt attattttaa tccacggttt ttaagcaatg atcttgctga atctgtcgat
     1981 ttatccgcct ttactaaggg acgcgaggca ccacctggta catatcgggt cgacatttat
     2041 ttgaacgacg aatttatgac tagtcgggat attactttta ttgcagatga taataacgca
     2101 gaactcatcc catgcctgag tacagatctt cttgtcagtc ttggtataaa aaaaatagcg
     2161 ttattagaca ataaagaaca ttcggcagaa aaacatgtgc cggacaacag cgcatgcaca
     2221 ccacttcagg atcgtttggc cgacgcatca actgagtttg atgttggtca gcaacatttg
     2281 tcactctccg ttccgcaaat ttatgttggc agaatggctc gcggctatgt ttctcctgat
     2341 ctgtgggaag aaggaataaa tgctgggctt ctaaactata gttttaatgg taattctatt
     2401 aataatcgta gtaaccataa tgcaggaaaa tccaactatg catatttgaa tttacagagt
     2461 ggcatcaaca ttggtagttg gcgactgcgc gataactcaa cgtggagtta taacagtggg
     2521 agtagcaatt catctgacag caataaatgg cagcatatca atacgtcggc tgaacgtgac
     2581 attattccct tacgctcacg gttaacggta ggtgatagtt ataccgatgg cgatattttt
     2641 gatagtgtga actttcgtgg cctcaaaata aattcaacag aagcgatgtt gcccgatagc
     2701 caacatggtt tcgctccggt gattcatggt attgcccgtg gaaccgcaca agtgagtgta
     2761 aaacagaacg gatacgatgt ttatcagact actgttccac ccggcccttt tactattgat
     2821 gatatcaact ctgcggccaa tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc
     2881 agtattcaga cattatatgt tccttattcg tctgttccgg ttctccaacg tgctggatat
     2941 acgcgttatg cgcttgccat gggggaatat cgtagtggaa ataacctgca aagctctccc
     3001 aagttcatac aaggtagctt gatgcatggt ctggaaggaa actggacacc ttatggcgga
     3061 atgcaaattg cagaagatta tcaggccttc aaccttggta ttgggaaaga tttaggactt
     3121 tttggtgcct tttctttcga tatcacgcag gccaatacga cacttgcaga tggcacccgt
     3181 cacagtgggc aatcggttaa atccgtctac agcaaatcct tctaccagac aggaaccaat
     3241 atccaggtcg caggatatcg ctattctacg caaggttttt ataacttatc cgacagtgcc
     3301 tacagtcgaa tgagtggtta caccgtcaag cctcctaccg gagacaccaa tgagcagaca
     3361 caatttattg attattttaa tctgttctac agtaagcgtg gtcaggaaca aataagcatc
     3421 tctcagcagc ttggaaatta cggtacgaca tttttcagtg ccagtcgcca aagttactgg
     3481 aacacgtcac gcagcgacca gcaaatatca tttggattaa atgtgccgtt tggtgatatt
     3541 acgacttcgc tgaattacag ctattccaat aatatatggc aaaacgatcg ggatcattta
     3601 ctcgctttta cgcttaatgt tcccttcagt cattggatgc gtacagacag tcagtcggca
     3661 tttcgtaatt caaacgccag ttacagtatg tcaaacgatt tgaaaggcgg catgaccaat
     3721 ctatcggggg tttatggcac tctgctgccg gataataacc tgaattatag cgttcaggtc
     3781 ggtaacaccc acggaggtaa tacatcgtct ggcaccagtg gttacagttc tcttaattat
     3841 cgtggagctt atggtaatac taatgtcggt tacagtcgga gtggtgacag cagccagatt
     3901 tattacggaa tgagtggtgg gattattgct catgctgatg gcatcacctt tggacagccg
     3961 ctgggcgaca caatggttct ggttaaggct cctggtgctg ataatgtcaa aatagagaac
     4021 cagaccggaa ttcataccga ctggcgtggc tatgccatat taccatttgc gacagaatat
     4081 agagaaaacc gtgttgctct taacgcgaat tcccttgcag ataatgttga actggatgaa
     4141 accgtgatca ctgtcatccc aactcacggt gctattgcca gagcaacatt taatgcacaa
     4201 atcggcggga aagtattaat gacgttgaag tacggtaata agagtgttcc attcggtgca
     4261 attgtcacac acggagagaa taaaaatggc agcattgtcg cagaaaacgg tcaggtttat
     4321 ctgactggac ttccacagtc agggaaatta caggtttcat ggggcaaaga taaaaactca
     4381 aactgtattg tcgagtacaa gcttcctgaa gtttctcctg gtaccttact gaaccagcag
     4441 acagcaatct gtcgctaagg aacaatagat tatgaaatac aataacatta ttttcctcgg
     4501 tttatgtctg gggttaacca cctattctgc tttatccgca gatagcgtta ttaaaattag
     4561 cgggcgcgtc ctcgattatg gctgcacagt ctcatcggat tcgcttaatt ttaccgtaga
     4621 tctccaaaaa aacagtgcca gacaatttcc aacgaccggt agcacaagtc cagccgtccc
     4681 ttttcagatt acgttaagtg aatgcagcaa agggacaacg ggggttcggg ttgcatttaa
     4741 cggtattgag gatgcagaaa ataatacttt gttgaaactg gatgaaggaa gcaatacggc
     4801 ctccggtttg ggtatagaaa tattggacgc aaatatgcgt ccggtgaaac tgaatgacct
     4861 tcatgccggg atgcagtgga tcccactggt accagaacag aacaatattt tgccttactc
     4921 cgctcgtctg aagtcaactc agaagtccgt caatccggga ctggtgaggg cttcggcaac
     4981 ctttaccctt gaatttcaat aatcagagtc agatatgaaa agacttcaca agaggttcct
     5041 gttagctacg ttttgcgcgt tattcacagc aactctccag gccgccgatg tcactatcac
     5101 tgttaatggt cgggtagtcg ctaaaccctg cactattcaa accaaagaag ctaacgttaa
     5161 tctcggggat ctttatacgc gcaatctgca acaacctggt tctgcatctg gctggcacaa
     5221 tattactttg tcattaaccg attgtccggt tgaaacaagt gcagtgacgg caatcgtgac
     5281 aggttcaact gacaatacgg gttattacaa aaatgaaggt actgccgaaa atattcagat
     5341 agagctgagg gatgaccagg atgctgcgtt aaaaaatggc gatagcaaaa cggttattgt
     5401 tgatgagatc actcgtaatg cacagtttcc acttaaggca agagctatca cggtgaatgg
     5461 aaacgcaagc cagggaacga tcgaggcgct aatcaatgtg atctacacct ggcaataacg
     5521 tcgattcgat tttcttagcc ttctttttaa atcaattttt aaccttagga tatatcatgg
     5581 gtaaaacaat cagtataaag gtcctgttcg gtatttatct tctattaatg gcaggcaaag
     5641 tttttgcctt cagttgtaat gtcgatggcg ggtcaagtat tggagcaggt acaacctctg
     5701 tttatgttaa tctcgaccct gtaatacagc cgggccagaa tctggttgta gacttgtctc
     5761 agcatataag ttgctggaat gattacggcg gctggtacga cactgatcat ataaacctgg
     5821 tacaaggctc agctttcgca ggctccctac agtcatataa aggatcgctg tactggaata
     5881 atgtgaccta ccccttcccg ttgacgacaa ataccaacgt attggatatt ggtgataaga
     5941 caccgatgcc attaccgtta aaactctata ttaccccagt tggggcggcc ggtggagttg
     6001 tcataaaagc gggggaagtc attgcccgta tacatatgta taaaattgca acattaggaa
     6061 gcggaaatcc gcgtaatttt acgtggaata ttatttcaaa taacaatgtt gttatgccaa
     6121 caggcggctg caccgttgat agtcgtaatg tcaccgtgga tctcccggac tttcctggaa
     6181 gcgcagaaat accccttggc gtatattgct caagcgaaca aaagctctca ttttatcttt
     6241 cgggtgccac cactgacagt agccgacaag tgtttgctaa cacagcacct gatgcaacaa
     6301 aagcatcggg agtgggtgtc actctaatgc gtaatggaaa aatactggct acgggagaaa
     6361 atgtctccct ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg
     6421 gacaaacagg aaacaaggtt tcagccggaa ccgtccagtc agtcattggt gtcactttta
     6481 tctatgaata
//
LOCUS       DAZFRJ010000225         6474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000225 DAZFRJ010000000
VERSION     DAZFRJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.227"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(219..1889)
                     /gene="betA"
                     /locus_tag="ACHKUN_004180"
     CDS             complement(219..1889)
                     /gene="betA"
                     /locus_tag="ACHKUN_004180"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO6662327.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1903..3375)
                     /gene="betB"
                     /locus_tag="ACHKUN_004181"
     CDS             complement(1903..3375)
                     /gene="betB"
                     /locus_tag="ACHKUN_004181"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO6662328.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3389..3976)
                     /gene="betI"
                     /locus_tag="ACHKUN_004182"
     CDS             complement(3389..3976)
                     /gene="betI"
                     /locus_tag="ACHKUN_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO6662329.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4105..6138
                     /gene="betT"
                     /locus_tag="ACHKUN_004183"
     CDS             4105..6138
                     /gene="betT"
                     /locus_tag="ACHKUN_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO6662330.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1338 a         1754 c         1764 g         1618 t
ORIGIN      
        1 acgcataata tatccagaaa ataaaatctg tcgcagataa ggttgtatta atagtctgta
       61 tcaggaatgt tcgggttaaa tatcagcaaa aagccagcat catggatact ggatatgaag
      121 catgagagtt acctcggtgt ttatataagg attcggtccc cctctctgga acggtaactc
      181 tcaatctgat cggttcctgc gttagttcac atcacgactc attttttcgc tctcaccggc
      241 atcccatttg ccacaaaata tcccgccgtg ctcctcggca gcgcttcctg tccacgaatc
      301 atatccgcta ttttctcgcc aatcataatt gtcgtggcgt tcaaattccc ggtgataatc
      361 tgcggcataa tcgacgcatc caccacacgc aggccttcta acccgtgtac gcggccttcg
      421 ccgtcaacca cggacatctc gtcgtaaccc attttgcagg taccgcacgg atggaaggcg
      481 gtttcggcgt ggttacgcac gaactcatcg agctgttcat ccgtctgaca ttccgtgccg
      541 gggctgattt cgcggccacg atactgatcc agcgcgggtt gatgcatgat ctcgcgggtg
      601 atgcgaattg cgtcgcggaa ctcctgccag tcctgctcgt gcgacatgta gttaaacaga
      661 atcgccggat gctggtgcgg gtcgcgggat ttaatccgca catgcccacg gcttggcgag
      721 cgcattgagc cgacgtggca ctggaaaccg tgctctttca ctgcattcga gccgttatag
      781 ttaatcgcta ctggcaggaa atggtactga atattcggcc acgcaaattc ctcacggctg
      841 cgaataaatc cacctgcttc aaagtggttg ctggcaccaa cgccagtgcc gccaaacagc
      901 cactccgcac cgattttcgg ctggttccac cactgcaggg cagggtagag ggaaaccggt
      961 tctttgcact catattgcag atacatctcc agatgatcct gaagattttc gccgacgccg
     1021 ggtaattcat gcaccagcgg aatatcaaac tccgccagca gttcagcgtt gccgacgccg
     1081 gagcgttgca ggatctgcgg tgaggcaatc gcgcctgcac ataacagcac ttctttgttg
     1141 gccgttgcgc gggttgggat ggtgctgtcg ccttccagcc attcgacgcc caccgcgcgt
     1201 ttgccgtcaa aaatgatgtg atcggtcata gcgtgaatac gaatggtcag gttagggcgc
     1261 gatttggcct gatcgagata accgcgcgcg gtactggcgc gacggccctg cggcgtgacg
     1321 gtgcgatcca tcggaccaaa accttcctgc tgataaccgt tgagatcgtc cgtgcgcggg
     1381 tagcccgcct gcacgcccgc ttcaatcatc gcttcaaaca gcggattgac gccgggtttg
     1441 gaggtagtga cgctcaccgg gccatcgcca ccgtggtagt cgttctcgcc cacatcgcga
     1501 gtctcggcct tgcggtagta gggcaggcag tcgagataac tccagttctc cagaccgggt
     1561 tcttgcgccc agttatcgag atccagcgca ttgccacgga tgtagcacat gccgttgatc
     1621 agcgacgatc cacccaggcc tttaccgcgt ccgcactcca tgcggcggtt attcataaac
     1681 ggttcaggct ctgtttcata ggcccagttg tagcgtttgc cctgtagtgg gaacgccagc
     1741 gctgcgggca tctgggtgcg gaagtcaaag cgatagtccg ggccgcccgc ttcaagcagc
     1801 agcacggagg tattcggatc ttcagtcaga cgggtagcga gaacgttgcc ggctgagccg
     1861 gcaccaataa tgatgtagtc aaattgcaaa taaacctcct ggttagaata tggactggaa
     1921 tttagccatc tcaacctgga tggacttcac ctgggtgtaa ctctggagcg tcatcacgcc
     1981 gttctcgcga ccaatgccgg agtgtttgta gccgccaacg ggcatctctg ccggggattc
     2041 gccccaggtg ttgatccagc aaatacccgc ttccagctga tgaatgacgc gatgcgcgcg
     2101 gttcaggtcc gccgtcacga tgcccgccgc caggccgtag tcggtatcat tggcgcggcg
     2161 aatcacttcg tcctcagatt cataagtgag aagcgacatc accggaccga agatctcttc
     2221 gcgcacgatg gtcatttcgt cgcggcaatc ggtgaacacg gtcggtgcaa cccatgcgcc
     2281 gttatcgaag ccatcgcctt tcagtacatc gccgccgcac agtacgcgcg cgccttcctc
     2341 tttgcctttg gcgatatagc gcagcacgtt atcgcgatgc gggaagctga ccagcgggct
     2401 gaagttagtt tgcggatcga aaacgtcgcc cgcgcgaatg cgctcaacgc gcgccagaat
     2461 tttctgctca aatgcggctt tgcatttcgc cggaacgaag acgcgggtgc cgttggtaca
     2521 cacctgaccg gagctgaaga agttcgccat catggcgata tcggcggcga gatcgagatc
     2581 cgcatcgtcg aaaacgatca gcggtgattt accgcccagt tccatggtca cttctttcag
     2641 ggaagaggcc gccgagttag ccatcacttt tttgccgctg gcgacaccgc cggtaaatga
     2701 cactttggca atgcccggat gctcggtcag atattgcccg gtctccgcgc ccccccccgg
     2761 caacacgtta aatacgccgt ccggcaggcc cgcttcgctg taaatttcag ccagctttaa
     2821 cgcggtaagc ggggtgactt cgctcggttt gaaaatcatt gcgttgcctg ccgccagcgc
     2881 cggggcggat ttccacaggg caatctggat cgggtagttc catgcgccaa tccccgccac
     2941 cacacccagc ggttcgcggc gggtataaac aaatgacgtc tcacgcaacg ggatctggct
     3001 gccttccagc gccgggatca gcccggcgta gtactccagc acgtccgcac cggtaacgat
     3061 atcgacggtt gaggtttccg aatatgcttt tccggtatcg agggtttcca gttttgcgag
     3121 ttcgtcattg cgttcacgca gaatatcaac ggcccgacgc agaatacgcg agcgctccat
     3181 agcggtcatc gccgcccaga ttttttgccc ctgttgggca ctcttcacgg cgcgatcgac
     3241 atcctcacgc ccggcggcct gcacggtcgc cagcacgtta ccgttggccg ggttaatggt
     3301 ctcgaaggtg cgaccgctgg tggcggaggt ataaccacca tgtatataaa gctgctgttc
     3361 tgccattcgg gacatcacgt ctcctcggtt aatcggtggg taaatgctga gtaataaagt
     3421 gacgggtcag cgagtgtgcg agaggtttat ccagcggttt gccgctcagt gccgcgcgca
     3481 gccataatcc gtcaatcagc gcggcaaggc cgtagcccgc ttcctgtgcc tgttggcgcg
     3541 gtaactcgcg gcgaaattcg ctcaccagat tcgacaataa gcggcggcta ctgacctgct
     3601 gtaaacgata gagcatcggc tgatgcatac tgctggccca gaacgccagc caggctttca
     3661 tcgccgcact gctcacctgc gtttcatcga agtttccgcc aacaatcgcc tgtaatcgct
     3721 gctctgcact gccctgcgga agtgcatgta atcgattcaa aaccgcgtca cgcagctgac
     3781 tggtgatatc gcgcatggtt gcttccagca gaccattttt gtccctgaaa tagtggctga
     3841 tgatccccgt agaaacgcct gcacggcggg cgatctgcgc gatcgttgca tcgtgcatgc
     3901 ccacttcatt tattgcttcc agtgtggcgt cgatcagttg tctgcgccgg atcgactgca
     3961 tccccaattt gggcattttc gccactccat tcatcagcgt tgtttatcta ttaaagcggt
     4021 tattgattgg acgttcaata taaaatgtgt cttaattgtt acgaatttga ttttaaatgg
     4081 taacaataac agtggggata ctggatgaca gacctttcac acagcaggga aaaggacaaa
     4141 atcaatccgg tggtgtttta cacctccgcc ggactgattt tgttgttttc cctgacaacg
     4201 atcctgtttc gcgacttctc ggccctgtgg attggccgca cgctggactg ggtttctaaa
     4261 accttcggtt ggtactatct actggcggca acgctctata ttgtctttgt ggtctgtatc
     4321 gcttgttcgc gttttggttc ggtgaagctc gggccagaac aatccaaacc ggaattcagc
     4381 ctgctgagtt gggcggcgat gctgtttgct gccgggatcg gtatcgacct gatgttcttc
     4441 tccgtagccg aaccggtaac gcagtatatg cagccgccgg aaggcgcggg acagacgatt
     4501 gaggccgcgc gtcaggcgat ggtctggacg ctgtttcact acggcttaac cggctggtcg
     4561 atgtatgcgc tgatgggcat ggcgctcgga tactttagct atcgttataa tttgccgctc
     4621 accatccgct cggcgctgta cccgatcttc ggtaaacgga ttaacgggcc gatagggcac
     4681 tcagtggata ttgcagcggt gatcggcacc atcttcggta ttgccactac gctcggtatc
     4741 ggtgtggtgc agcttaacta tggcttgagc gtactgtttg atattcccga ttcgatggcg
     4801 gcaaaagcgg cactgatcgc cttgtcggtg ataatcgcca cgatctctgt cacctccggt
     4861 gtcgataagg ggattcgtgt gttatcggaa cttaacgttg cgctggcgct gggattgatc
     4921 ctgttcgtat tgtttatggg cgacacctcg ttcctgctta atgcgctggt gctgaatgtt
     4981 ggcgactatg tgaatcgctt tatgggcatg acgctcaaca gttttgcctt cgaccgtcca
     5041 gttgagtgga tgaataactg gacgctcttc ttctgggcat ggtgggtggc atggtcgccg
     5101 tttgtcggct tgttcctggc gcgtatctcg cgtgggcgta ccattcgcca gttcgtgctg
     5161 ggcacgttga ttattccgtt taccttcacg ctgttatggc tctcggtgtt cggcaatagc
     5221 gcgctgtatg aaatcatcca cggcggcgcg gcatttgccg aggaagcgat ggtccatccg
     5281 gagcgcggct tctacagcct gctggcgcag tatccggcgt ttacctttag cgcctccgtc
     5341 gccaccatta ctggcctgct gttttatgtg acctcggcgg actccggtgc gctggtgctg
     5401 gggaatttca cctcgcagct taaagatatc aacagcgacg cccccggctg gctgcgcgtc
     5461 ttctggtcgg tggcgattgg cctgctgacg ctcggcatgc tgatgaccaa cgggatatcc
     5521 gcgctgcaaa acaccacggt gattatgggg ctgccgttca gctttgtgat cttcttcgtg
     5581 atggcggggt tgtataaatc tctgaaggta gaagattacc gccgtgaaag tgccaaccgc
     5641 gataccgcac cgcgaccgct ggggcttcag gatcgcctga gctggaaaaa acgtctctcg
     5701 cgcctgatga attatccggg cacgcgttac actaaacaga tgatggagac ggtctgttac
     5761 ccggcaatgg aagaagtggc gcaggagctg cggttgcgcg gcgcatacgt ggagctaaaa
     5821 agcctgccgc cggaagaggg acaacagttg gggcatctgg atttgttggt gcatatgggc
     5881 gaagaacaaa actttgtcta tcagatttgg ccgcagcaat attcggtgcc gggctttacc
     5941 taccgcgcac gtagcggtaa atcgacctac taccggctgg aaaccttcct gttagaaggc
     6001 agccagggca acgacctgat ggactacagc aaagagcagg tgatcaccga tattcttgac
     6061 cagtacgagc ggcaccttaa ctttattcat ctccatcgtg aagcgccggg ccatagcgtg
     6121 atgttcccgg acgcgtgatt gtttttgcat aaaaccgcgc catgtctgca tatggcgcgg
     6181 gaatcattga ctcgctgtaa aaaattatgc atatattata tatgattgtc atgtcatatc
     6241 ctgcaattcc tcttgtatca cttcatgatt ttatatttta ttaattaacg attttatttt
     6301 gtattagctg aatggattca gtcctatagt gttatatatt tattaataag acgattctta
     6361 tattttgata cctaataaga gttaccgctt ttatcggctg tatattaaat gtattcctct
     6421 tgcttttgtt tatattttga gttgatttga gttcctggat acatataatg cgca
//
LOCUS       DAZFRJ010000226         6462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000226 DAZFRJ010000000
VERSION     DAZFRJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.228"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1972
                     /gene="acnA"
                     /locus_tag="ACHKUN_004184"
     CDS             <1..1972
                     /gene="acnA"
                     /locus_tag="ACHKUN_004184"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO6662331.1"
                     /translation="AMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHG
                     VVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQV
                     ELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAA
                     SNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKK
                     AVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPL
                     PDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLA
                     AEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTR
                     SDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKP
                     DSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLP
                     DSDVVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERI
                     HRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQE
                     VVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2036..2626)
                     /gene="ribA"
                     /locus_tag="ACHKUN_004185"
     CDS             complement(2036..2626)
                     /gene="ribA"
                     /locus_tag="ACHKUN_004185"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO6662332.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2796..3560
                     /gene="pgpB"
                     /locus_tag="ACHKUN_004186"
     CDS             2796..3560
                     /gene="pgpB"
                     /locus_tag="ACHKUN_004186"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO6662333.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3709..4017
                     /gene="lapA"
                     /locus_tag="ACHKUN_004187"
     CDS             3709..4017
                     /gene="lapA"
                     /locus_tag="ACHKUN_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO6662334.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4024..5193
                     /gene="lapB"
                     /locus_tag="ACHKUN_004188"
     CDS             4024..5193
                     /gene="lapB"
                     /locus_tag="ACHKUN_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO6662335.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5385..6122
                     /gene="pyrF"
                     /locus_tag="ACHKUN_004189"
     CDS             5385..6122
                     /gene="pyrF"
                     /locus_tag="ACHKUN_004189"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO6662336.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6122..6448
                     /gene="yciH"
                     /locus_tag="ACHKUN_004190"
     CDS             6122..6448
                     /gene="yciH"
                     /locus_tag="ACHKUN_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO6662337.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         1571 a         1520 c         1762 g         1609 t
ORIGIN      
        1 cgcaatgtta ggccagccgg tttccatgct tatcccggac gtagtgggct ttaaacttac
       61 cggtaaatta cgtgaaggta ttaccgccac agacctggtt ctcactgtta cccaaatgct
      121 gcgcaaacat ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc tggattcact
      181 accgctggcg gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg
      241 cttcttccca atcgatgctg taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga
      301 tcaggttgag ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta acccgggcga
      361 tgaaccgatt tttaccagta cgttagaact ggatatgaat gacgttgaag cgagcctggc
      421 agggccaaaa cgcccacagg atcgcgttgc actgcccgat gtaccaaaag catttgccgc
      481 cagtaacgaa ctggaagtga atgccacgca taaagatcgc cagccggtcg attatgttat
      541 gaacggacat cagtatcagt tacctgatgg cgctgtggtc attgctgcga taacctcgtg
      601 caccaacacc tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt
      661 aactctgggc ctcaagcggc aaccgtgggt caaagcgtcg ctggcaccgg gttcgaaagt
      721 cgtttctgat tatctggcaa aagcgaaact gacaccgtat ctcgacgaac tggggtttaa
      781 ccttgtggga tacggttgta ccacctgtat tggtaactct gggccgctgc ccgatcctat
      841 cgaaacggca atcaaaaaag gcgatttaac cgtcggtgcg gtgctgtccg gcaaccgtaa
      901 ctttgaaggc cgtatccatc cgctggttaa aactaactgg ctggcctcgc cgccgctggt
      961 ggttgcctat gcgctggcgg gaaatatgaa tatcaacctg gctgctgagc ctattggtca
     1021 tgatcgtaaa ggcgacccgg tttatctgaa agatatctgg ccatcggcac aagaaattgc
     1081 ccgtgcggta gaacaagtct ccacagaaat gttccgcaaa gagtacgcag aagtttttga
     1141 aggcacagca gagtggaagg aaattaacgt cacacgatcc gatacctacg gttggcagga
     1201 ggactcaacc tatattcgct tatcgccttt ctttgatgaa atgcaggcaa caccagcacc
     1261 agtggaagat attcacggtg cgcggatcct cgcaatgctg ggggattcag tcaccactga
     1321 ccatatctct ccggcgggca gtattaagcc cgacagccca gcgggtcgat atctacaagg
     1381 tcggggtgtt gagcgaaaag actttaactc ctacggttcg cggcgtggta accatgaagt
     1441 gatgatgcgc ggcaccttcg ccaatattcg catccgtaat gaaatggtgc ctggcgttga
     1501 aggggggatg acgcggcatt tacctgacag cgacgtagtc tctatttatg atgctgcgat
     1561 gcgctataag aaggaacaaa cgccgctggc ggtgattgcc gggaaagagt atggatcagg
     1621 ctcaagccgt gactgggcgg caaaaggtcc tcgtctgctt ggaattcgtg tggtgattgc
     1681 cgaatcgttt gaacgaattc accgttcgaa tttaattggc atgggcatcc tgccgctgga
     1741 atttccgcaa ggcgtaacgc gtaaaacgtt aggactaacc ggggaagaga agattgatat
     1801 tggcgatctg caaaatctac aacccggtgc gacggttccg gtgacgctta cgcgcgcgga
     1861 tggtagccag gaggtcgtac cctgccgttg tcgtatcgac accgcgacgg agttgaccta
     1921 ctaccagaac gacggcattt tgcattatgt cattcgtaat atgttgaagt aacaagtatt
     1981 tgcttgccgg ttattttgct tccggcaagc aaattaatta cacaatgcaa gagggttatt
     2041 tgttcagcaa atggcccatt ttctcggctt tggtatcgag ataatgttcg ttattggggt
     2101 tacgacctac aatcaatggt acgcgttcaa caatattaat ccctgcttcg gtcagaattt
     2161 cgactttttt cgggttattg gttaacaagc ggacttcatt gacgccaagg agtttgaaca
     2221 tatcagcgca aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg tggttagcct
     2281 ctacggtatc gtaaccttga tcctgcagtg cgtaagcgcg gattttattc agcagaccaa
     2341 tgttacgacc ttcctgacgg tgatacagca aaataccacg gccttcctcg gcaatttgcg
     2401 tcaatgccgc ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg
     2461 tcagacattc ggaatggacg cgcgcaagta ccggggtatg cccggaaata tcgccataga
     2521 ctagcgcgac atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc
     2581 cccatggggt tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa ttctccagat
     2641 aatgctggtt ctgtattggc ttattttgcc ataacgagaa ggggttcacc taatcacgac
     2701 gcgtcgatcg ttcacggaat ggcacaaatc tgtcaatttt tcctggaact ggcgttttca
     2761 gttatgattg tgggacttat caaaaaggag aggccatgcg ttcgattgcc agacgtaccg
     2821 cagtgggagc tgcactattg cttgtcatgc cagtagccgt atggatttct ggctggcgtt
     2881 ggcaacctgg agaacaaagt tggctactaa aagcggcttt ttgggttact gaaactgtca
     2941 cccagccctg gggcgtcatt acacatttga ttttattcgg ctggtttctc tggtgtctgc
     3001 gttttcgcat taaggctgcc tttgtattat ttgccattct ggcggccgca atccttgtgg
     3061 gacaaggcgt taaatcctgg atcaaagaca aagtccagga accacgacct tttgttatct
     3121 ggctggaaaa aacacatcat attccggttg atgagttcta cactttaaag cgagcagaac
     3181 gcggaaatct agtgaaagaa cagttggctg aagagaaaaa tatcccacaa tatttgcgtt
     3241 cacattggca gaaagagacg gggtttgcct ttccttccgg tcacacgatg tttgctgcca
     3301 gttgggcact gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta
     3361 tcttgctggt ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg atgcattggc
     3421 cacgcgatct ggtagtagct acgttgattt cgtgggcgct ggtggcggtg gcaacgtggc
     3481 ttgcgcaacg aatttgtggg ccattaacac cacctgcgga agaaaatcgc gaaatagcgc
     3541 aacgagaaca agaaagttaa cgctggttga ttttccgaat ttagccctta aatcatcaac
     3601 aatgcgcgtg gatgccattt cgcagacggc gcgaaaatgg tactttaaag ggctattgcg
     3661 gtaagttgac cataatttat tcgctctaac cacataacgg gaagtaatgt gaaatattta
     3721 ctcattttct tactggtgtt agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac
     3781 gatcaacagg tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat ttccacattg
     3841 ctggcggtat tgtttgctgc ggggtttgct atcggttggt tgatttgtgg cctgttctgg
     3901 ctgcgagttc gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag
     3961 ctttcacccg cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt
     4021 tctatgctgg agttgttgtt tctgcttttg cctgtagccg cggcctatgg ctggtatatg
     4081 ggccgcagaa gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc gcgtgattac
     4141 gtagcggggg ttaacttcct gcttagtaat caacaggata aagcggtaga cctgtttctc
     4201 gatatgctta aagaggatac aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc
     4261 cgttcgcgtg gcgaagttga tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc
     4321 tcgctgacct atgaacagcg tctgttggcg attcaacaac tagggcgtga ttacatggcc
     4381 gccgggttat acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga aactgacttc
     4441 cgcattggcg ctctgcaaca gttgctacaa atctaccagg ctaccagtga gtggcagaaa
     4501 gcaattgatg ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt
     4561 gcccatttct actgtgagtt agccctgcag catatggcca gcgacgatct cgatcgtgcc
     4621 atgaccttgc taaaaaaagg ggcggcggca gataaaaaca gcgcccgcgt atccatcatg
     4681 atgggacgcg tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc
     4741 gtgatatccc aggacagaga actggtcagc gaaacgctgg aaatgttgca aacctgctat
     4801 cagcagttgg gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt ggaagagaac
     4861 accggtgccg atgctgaatt gatgcttgct gatatcatcg aagcgcgcga cggtagtgag
     4921 gccgcacagg tctatattac gcgccagctt cagcgtcatc cgaccatgcg tgtgttccat
     4981 aagttaatgg attaccactt aaatgaagcg gaagaagggc gtgccaaaga gagcctgatg
     5041 gtgctgcgtg acatggttgg cgaaaaggta cgtagtaagc ctcgttatcg ctgccagaaa
     5101 tgtggtttta ccgcatacac cctctactgg cattgtccgt cttgtcgggc ctggtcaacc
     5161 attaaaccga ttcgcggtct tgatggcctg taatttttaa aaaatcctac tttagttaca
     5221 acatactagt tattgatgtt ccattgtgct ccggcaacga cggcgcagaa aaagcctgcc
     5281 aggggaaaaa tcgcaactgt taatttttta tttccacggg tagaatgctc gccgtttacc
     5341 tgtttcgcgc cacttccggt gcccatcatc aagaaggtct ggtcatgacg ttaactgctt
     5401 catcttcttc ccgcgctgtt acgaattctc ctgtggttgt tgcccttgat tatcataatc
     5461 gtgatgacgc gctgtccttt gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg
     5521 gcaaagagat gtttacattg tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt
     5581 ttgatatctt tcttgacctg aaattccacg atattcctaa cactgctgcg cacgctgtcg
     5641 ctgctgcagc tgacttaggc gtgtggatgg tgaatgttca tgcctctggt ggggcgcgta
     5701 tgatgaccgc agcgcgtgag gcactggttc cgtttggcaa agatgcaccg cttttgattg
     5761 ctgtgacagt gttgaccagc atggaagcca gcgacctggt cgatcttggc atgacactgt
     5821 cacctgcaga ttatgcagaa cgtctggcgg cactgacgca aaaatgtggc cttgatggtg
     5881 tggtgtgttc tgctcaggaa gctgtgcgct ttaaacaggt attcggtcag gagttcaaac
     5941 tggttacgcc gggcattcgt ccgcagggga gtgaagctgg tgaccagcgc cgcattatga
     6001 cgccagaaca ggcgttgtcg gctggtgttg attatatggt gattggtcgc ccggtaacgc
     6061 aatcggtaga tccagcgcag acgctgaaag cgatcaacgc ctctttacag cggagtgcat
     6121 gatgagtgat tccaacagcc gtctggtcta ctcaacggaa accggacgta ttgatgagcc
     6181 caaagcggcc cctgtacgcc ctaaaggcga cggtgtggtg cgtattcagc gtcagaccag
     6241 tggacgtaaa ggtaagggcg tttgcctgat taccggtgtc gatctcgatg atgccgaact
     6301 gacaaaactt gcagcggaac tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg
     6361 agttattgaa atccagggcg ataagcgtga tttattaaaa tcactacttg aagcgaaagg
     6421 gatgaaggta aaactcgcag gcggttaaca taaaaagcca cg
//
LOCUS       DAZFRJ010000227         6369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000227 DAZFRJ010000000
VERSION     DAZFRJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.229"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..279
                     /gene="nleC"
                     /locus_tag="ACHKUN_004191"
     CDS             <1..279
                     /gene="nleC"
                     /locus_tag="ACHKUN_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO6662338.1"
                     /translation="RLRNLNALRQAAMRHEKNERAFFERLGTISDRYEASPDFTEYSA
                     VSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVDRHN"
     gene            complement(400..483)
                     /locus_tag="ACHKUN_004192"
                     /pseudo
     CDS             complement(400..483)
                     /locus_tag="ACHKUN_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            568..654
                     /locus_tag="ACHKUN_004193"
                     /pseudo
     CDS             568..654
                     /locus_tag="ACHKUN_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(651..899)
                     /locus_tag="ACHKUN_004194"
     CDS             complement(651..899)
                     /locus_tag="ACHKUN_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6662339.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1125..1220)
                     /locus_tag="ACHKUN_004195"
                     /pseudo
     CDS             complement(1125..1220)
                     /locus_tag="ACHKUN_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1217..1783)
                     /gene="yecD"
                     /locus_tag="ACHKUN_004196"
     CDS             complement(1217..1783)
                     /gene="yecD"
                     /locus_tag="ACHKUN_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6662340.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2093..3865
                     /gene="aspS"
                     /locus_tag="ACHKUN_004197"
     CDS             2093..3865
                     /gene="aspS"
                     /locus_tag="ACHKUN_004197"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO6662341.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            3983..4435
                     /gene="nudB"
                     /locus_tag="ACHKUN_004198"
     CDS             3983..4435
                     /gene="nudB"
                     /locus_tag="ACHKUN_004198"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO6662342.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4464..5204
                     /gene="yebC"
                     /locus_tag="ACHKUN_004199"
     CDS             4464..5204
                     /gene="yebC"
                     /locus_tag="ACHKUN_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6662343.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5239..5760
                     /gene="ruvC"
                     /locus_tag="ACHKUN_004200"
     CDS             5239..5760
                     /gene="ruvC"
                     /locus_tag="ACHKUN_004200"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO6662344.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(5762..6364)
                     /gene="yebB"
                     /locus_tag="ACHKUN_004201"
     CDS             complement(5762..6364)
                     /gene="yebB"
                     /locus_tag="ACHKUN_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO6662345.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1612 a         1519 c         1694 g         1544 t
ORIGIN      
        1 agactacgta acctgaatgc ccttcgacag gctgccatga ggcatgaaaa gaatgagagg
       61 gctttcttcg aaagactggg tacgatcagt gaccgatatg aggcgagtcc tgatttcaca
      121 gagtattccg ctgtgtctaa cataggatac ggatttatcc agcaacatga ttttccaggg
      181 gtggctatcg acgagaattt acaggatgta aatcagatcc aactgtatca tggtgctcct
      241 tatatcttta catttgggga tgtggacaga cacaattagc gctgattcgt ctttacagtt
      301 acatagggtt actactcata gatattaacg gagttgatga tgggcaatcg tgcaacattg
      361 tattgtatgt aaaggcagag ccccgaattt tactgttgta agtgggcctg gcgggaatgg
      421 tatcgccagc atcattggct tgggtccagt aaaaagagac gcagaaagat gcacaatagg
      481 cactacgcgt catgcatgga ttaggtcgct catcatttac acagtactgc taaaaaatta
      541 ttaaggaaag cgcggcactt cgtatgtaag aacgtgtcgc ggttggctgg tgaactttcg
      601 atagtgcgag tattgaatga tttccagccg ttaccgattt tacgtgttta ttagtgaaca
      661 aaccactcgt cagcagactc ccaggtatct ttcagagtct cctgaacaaa tgtttttgca
      721 gaatctttat ctgcggtgcg tgtaacagaa agcccatcgt tgctggtggc ttttacgatc
      781 acctctacat cgtcataacg tttactgatg cgtcgggtta attcttcctt taacgcatcc
      841 acagcaccgt ttggcatttt gctgattttt tctttagcaa tacagatctc aacacgcata
      901 acaccctcct gtaactgtgg ttatatacag gtgttatttt tatctgtatg gataaccagt
      961 gtcaattcct gatattgttt atggggcatc aatggggcat gtatgggaca ctttttatcg
     1021 gcgaaattcg tcgaagttcg tcgacatggt aaacgaatca tctatccaac ccttgaaaaa
     1081 cggcgctcct ggacgatctt cgtcgatttt taaaaatgtt gcgtcatgca gttaaagtgg
     1141 cgggcatact cttcaaggct ggtgatcccc aaccgcaccc atttaggatg cgaccattgc
     1201 ggtagaccga tgtaaatcat aacgcgtgaa ggatctcttc cacgctacgc acacgggcga
     1261 tgcgtgggta gatatggttg atgctgttat tgtgctgctc ggcgctagcg gcactacagg
     1321 cgtcttcggc aatcaccaga ttaaagccaa gttcccaggc attgcgggcg gtggattcaa
     1381 caccgatatt ggtcgagatc ccacataaca ctattgtatc gatacctcgg cggcgcagtt
     1441 gcaactccag atccgtaccg taaaacgcac cccactgacg tttgatgatt tcgatatcac
     1501 tgtcagttgc acctaatgca gctggatgtt gccaccagtt ttcgggcaac actttagcgg
     1561 gggagggggc atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaacgc
     1621 gcaccagaaa cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat
     1681 taactacctc atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat
     1741 cgatcaccac cagcgcagtg gttttagcat taagttcaag cataatgact cctgaaaaaa
     1801 caaaatcgtg cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa
     1861 tttttatgag ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc
     1921 gatataaagt ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt
     1981 tccagtataa tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg
     2041 gtcacctgcg cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac
     2101 agaatattgt ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg
     2161 ggtcaaccgt cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg
     2221 tatcgtgcag gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact
     2281 gcgtaatgag ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat
     2341 taaccgcgat atggcgaccg gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa
     2401 ccgcgcagat gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa
     2461 ataccgctac ctcgacctgc gtcgtccgga aatggctcag cgcctgaaaa cccgcgctaa
     2521 aatcaccagc ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc
     2581 gatgctgacc aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca
     2641 caaaggtaaa ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat
     2701 gtccggtttt gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc
     2761 tgatcgtcag cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca
     2821 agtgcgtgaa gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga
     2881 tctgggcgat ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa
     2941 accggatctg cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga
     3001 gtttgcagta tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt
     3061 tccgggtggc gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat
     3121 ctacggcgcg aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg
     3181 tatcaacagc ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg
     3241 tactgccgcg caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc
     3301 cgacgcgatg ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag
     3361 caaatgggca ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg
     3421 cctgacggca atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa
     3481 agctgcaccg gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt
     3541 gggcggtggt tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct
     3601 gggtatcaac gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata
     3661 cggtactccg ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac
     3721 tggcaccgac aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct
     3781 gatgactgaa gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt
     3841 tgtgaagaag gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat
     3901 caaggcggca actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga
     3961 aggcagtcaa cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt
     4021 agtggtcatc tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc
     4081 cgatttctgg cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc
     4141 catgcgcgaa gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat
     4201 tgactgtcag cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc
     4261 gggcgtgacg cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat
     4321 cgttttcact gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac
     4381 taagtcctgg agcaaccggc aggcgattga acagtttgtc attaacgctg cctgaacagg
     4441 cagggctatc tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc
     4501 gtaaagctgc gcaggatgca aagcgcggta aaatcttcac taaaatcatt cgtgagctgg
     4561 taaccgcggc taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcagcaa
     4621 ttgataaagc actgtctaac aacatgaccc gcgacacact gaaccgcgca attgcacgtg
     4681 gtgtgggcgg tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg
     4741 gcggcacggc aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag
     4801 tgcgtcatgc attcagcaaa tgtggcggta acctcggtac tgatggttcc gtagcctatc
     4861 tgttcagcaa aaaaggcgtg atctccttcg agaaaggcga tgaagacacc atcatggaag
     4921 cagctttgga agcaggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct
     4981 acaccgcatg ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag
     5041 cagacagcgc ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg
     5101 caccgaaact gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt
     5161 accataacgg tgaaatctct gatgaggttg cagcgactct ctgatgaggc ctgctaaaca
     5221 gcaaaacgga gacgcgtgat ggctattatt ctcggcattg atccgggttc gcgcgtgacc
     5281 ggctatggcg tcatccgcca ggtaggcagg caactgtcct acctgggtag tggatgcata
     5341 cgcaccaaag tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa
     5401 atcatcaccc agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac
     5461 gctgactcag ccctgaaact gggccaggcg cgcggcgtgg cgattgtggc ggcggtgaat
     5521 caggaattgc cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatt
     5581 ggtagcgccg agaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct
     5641 aatccacaag cggatgccgc tgatgcgtta gctatcgcga ttacccattg tcacgtcagc
     5701 cagaatgcaa tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa
     5761 gttataccgc ctcggtcagt tcaggctgag gcgtttcaac tccctccgcg tgaatcaaca
     5821 ccaaacccgg atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag
     5881 aacctaagaa ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt
     5941 gtccaaatgt ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga
     6001 caaattttga acagaactgg cgggaagatt cgtatttaaa acccgtgtgg taaagtttgc
     6061 gtagccggga gggaacctgt tcaacaattc gttgtttctg ttgttctgtc agcccggcgt
     6121 ctaatcgctt tatagcatag cgttgattag cagagcgttt aataaaacgg gatagcgtag
     6181 tgatggttga gaggggaaca cggctttctg caaccagaaa gtcttcaccg ttatgaccga
     6241 taatgatccc gacgtgatta ctccagcagt ttgatgcagc tgatatttga ccaaataagg
     6301 cagcacctat acatgtaaag acgatatcac caatttcata ttcagcaggg taattaatat
     6361 tcacgttgt
//
LOCUS       DAZFRJ010000228         6362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000228 DAZFRJ010000000
VERSION     DAZFRJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.230"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..357
                     /gene="ydjO"
                     /locus_tag="ACHKUN_004202"
                     /pseudo
     CDS             1..357
                     /gene="ydjO"
                     /locus_tag="ACHKUN_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(361..1752)
                     /gene="tcyP"
                     /locus_tag="ACHKUN_004203"
     CDS             complement(361..1752)
                     /gene="tcyP"
                     /locus_tag="ACHKUN_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO6662346.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1885..2475)
                     /gene="ydjM"
                     /locus_tag="ACHKUN_004204"
     CDS             complement(1885..2475)
                     /gene="ydjM"
                     /locus_tag="ACHKUN_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6662347.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2638..3306)
                     /gene="hxpB"
                     /locus_tag="ACHKUN_004205"
     CDS             complement(2638..3306)
                     /gene="hxpB"
                     /locus_tag="ACHKUN_004205"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO6662348.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3453..3989
                     /gene="yniB"
                     /locus_tag="ACHKUN_004206"
     CDS             3453..3989
                     /gene="yniB"
                     /locus_tag="ACHKUN_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO6662349.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4030..4890)
                     /gene="yniA"
                     /locus_tag="ACHKUN_004207"
     CDS             complement(4030..4890)
                     /gene="yniA"
                     /locus_tag="ACHKUN_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO6662350.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4996..5286)
                     /gene="ghoS"
                     /locus_tag="ACHKUN_004208"
     CDS             complement(4996..5286)
                     /gene="ghoS"
                     /locus_tag="ACHKUN_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6662351.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5387..6316)
                     /gene="pfkB"
                     /locus_tag="ACHKUN_004209"
     CDS             complement(5387..6316)
                     /gene="pfkB"
                     /locus_tag="ACHKUN_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO6662352.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1634 a         1594 c         1565 g         1569 t
ORIGIN      
        1 ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa gtttccttcg
       61 ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc gggaatggga
      121 agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa acgtctcggg
      181 tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg tattgataag
      241 ttggacattt ttttcgctga cgatctgttt aaatatgagt cagtatgcat cgccgcgatg
      301 gaacatttaa aaagtaaagc aacggcccct atacaaaacg gaccgttgcc agcataagaa
      361 ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat cggtttgctt
      421 cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta gcgccgtacg
      481 gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta ctggcaggcc
      541 catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc
      601 tgcggaacta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt
      661 aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc
      721 gttctgacca atggttgcac cgaaagaggc ggcgaaactg gcgatggatt caggaacgcc
      781 cagacgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt
      841 aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac tcacgccatt
      901 aatgcccagc agaatgccat gcactgcaaa cataatcagc agaccgaggt aggacgcgac
      961 aacgaaactt cccagtttga tgatgtcttg caggttagaa cctgcaacca ctttggtcat
     1021 tagtgccaga acgccgtaag gggtcaactg catgaccagg cgaaccagtt tcatcaccca
     1081 gctttgcagg gtatcgatag cggttaagac gcgttcacct ttcggcgcat catccttcag
     1141 cagtttcagc gcagctacac cgaggaatgc ggcaaaaatt accacgctga taattgacgt
     1201 cggattggct ccggtcagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg
     1261 cggaacgctc aggtcagaga ctttaccaac atagttactt tcgatggcgt tcagacgtgc
     1321 agtttctgca cctccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag
     1381 cacaccgacc agcgccgcaa tcagtgtggt aaacaaaagc gtaccgatgg tcagaaaact
     1441 gattttgcct aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa
     1501 gactaacggc ataacgatca tttgcagcag ttgaacatag ccgttaccaa cgatgttaaa
     1561 ccactgtaca gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag
     1621 gccaaaaacc acacccatca ccagacccac cagcactttt ttcgccagac tccactgttt
     1681 gtggcgggtc tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa
     1741 tggaaagttc atccccgttc tcctgatctt attatttcta tcactgcgta gcagtcatag
     1801 tgttggcgca agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat
     1861 tccagaaagg tgatttttgc ctttttagta ttcaacctga tgcttaataa gccgatgaaa
     1921 ctggatctgc aaggtattga tcatttgcga tgaccaacga accgcgctgt tttcgggtaa
     1981 tgaatggggc atccataccg accagacaaa taatgccatg cagataaaac gttccagttg
     2041 gttgcccttt tggggaacca ggataggcaa gcggaaacgc cagcgacatg gccagagcag
     2101 gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg cttaaataac ccagcaccat
     2161 tccttgtagc gcatcagccg gaatgaacca gctttcagga accttaaggt aaaaggttgc
     2221 cagcagcgca aataccgcca gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc
     2281 gatcggtttt gatatccact ttaatcgctg cccaagaaac gactttgggt gatcgatgtc
     2341 cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag
     2401 cacgggcgtc agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg
     2461 accttccgcc gtcatgataa aactcactaa actgtcgata cgtacagtat aggattttta
     2521 tacagtgcac ggaagtggtg acggtgtaac tctttctctt aaattgacga ttaatccgtc
     2581 agttgaatgc aaattcagat aaagaaccag gggcagcgag atcactgccc ctggtcatta
     2641 accgagaagg tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac
     2701 aaaacgtgga tcattttgcg cttctggcgc aggaacgaca atggaacgca tgcgggctgc
     2761 tttagaggcg atcatgccat ttactgaatc ttccagcgct acgcaggtca gtgggtcaac
     2821 gcccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt aaggcagttt
     2881 ttcagccgag gcgagggcat cgaaactgtc gcgtaagtca aacatggtca acactttttc
     2941 cagcatatgt agtggtgacg cggaggccag tcccaccaat aaaccttgtt ctttgcataa
     3001 cgcaacggct tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc
     3061 acgggcaata acccgttcta ctacttcctg acggcttggc ccattccacg gttgccgggc
     3121 gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg
     3181 acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat cccataaagg
     3241 ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt
     3301 tgacattaca acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt
     3361 aagcgcctat catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa
     3421 gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg
     3481 ctgttttgaa aaggattttg ggttgggtga tttttatacc tgcattgatc tctacattga
     3541 tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca
     3601 tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc
     3661 tgttctggta taactccccg acgcccaatt ttaatggtgg cgtgaacgtt atgttctggg
     3721 tgattttcat cctgattttt gtcggactgg cgttgcagga ttccggtgcc cggatgagtc
     3781 gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag
     3841 gggaagaggg gctaacgcgt gaacaaattg agtcccgtat tgttgttccg caccatacta
     3901 ttttcctgca gtttttctca ctatatatcc tgccggttat ttgtattgct gcaggttatg
     3961 tgttcttttc tctgcttggg tttatttaat caaaaggccg cacggagcgg ccattatcct
     4021 caacccatat catgctgcta ataatctatc caatgactgc tgagcaataa ccaaatgctg
     4081 accgccaaat aatcttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc
     4141 aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg
     4201 ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggcagg
     4261 gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg
     4321 caacagagaa ggctgcggtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc
     4381 gtcgatattg ccgaaagcga tccctttctc tgctgccagt tccaactgcc agccaatccg
     4441 ttgttcagca aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga
     4501 gagcgagtta tcaaaatcga ggccaaattg tggttggtca ctccattgat gtaaacgcgc
     4561 aatttgctga ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc
     4621 catcaccaga aaactgtagt cacggtcagc gcctactgcc caaaccttag gcacggtgac
     4681 ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag
     4741 ttccctttca tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc
     4801 atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc
     4861 gctcaaaaga cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca
     4921 taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta
     4981 ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaggtcg tgatggtgat
     5041 ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc
     5101 ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc
     5161 atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat
     5221 ttcagtcaat gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact
     5281 ggccatagtt cacccctttg ttatcacatg acttttgagc atagtcggag aaacgcgttg
     5341 ccgacaggtt ggtggtgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt
     5401 aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg
     5461 ctgcactccc cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca
     5521 gtttcagtgt catcgcgccg accatgctgt caccagcgcc aacggtactc tggcttttca
     5581 ccggtggtgg caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac
     5641 caagggaaac gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc
     5701 ggacatcgtc tggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag
     5761 gcttaaccaa ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg ccagaactgt
     5821 cgatgatgca gcggatccct tggttttgcg cagcggaaat cagttgggtt aatttttcca
     5881 gcttcacacc tggcggcagg cttccgctta tgaccaggat ggccccggat tcaatttcca
     5941 gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa
     6001 aacgatactg ctcaccgctt gcttccacat gtacgtgtaa attctgccgg gtccagtctt
     6061 tggcttctac agtagcgacg gggacatttt catccgccaa cagagaaacc aggtgttcgc
     6121 cggtcgcgcc acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc
     6181 gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc
     6241 cttcgggata aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg
     6301 tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcc
     6361 ga
//
LOCUS       DAZFRJ010000229         6117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000229 DAZFRJ010000000
VERSION     DAZFRJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.231"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..834
                     /gene="exuR"
                     /locus_tag="ACHKUN_004210"
     CDS             58..834
                     /gene="exuR"
                     /locus_tag="ACHKUN_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO6662353.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1074..1157
                     /gene="yqiM"
                     /locus_tag="ACHKUN_004211"
     CDS             1074..1157
                     /gene="yqiM"
                     /locus_tag="ACHKUN_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO6662354.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1179..1841
                     /gene="yqjA"
                     /locus_tag="ACHKUN_004212"
     CDS             1179..1841
                     /gene="yqjA"
                     /locus_tag="ACHKUN_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO6662355.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1845..2228
                     /gene="mzrA"
                     /locus_tag="ACHKUN_004213"
     CDS             1845..2228
                     /gene="mzrA"
                     /locus_tag="ACHKUN_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO6662356.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2375..2743
                     /gene="yqjC"
                     /locus_tag="ACHKUN_004214"
     CDS             2375..2743
                     /gene="yqjC"
                     /locus_tag="ACHKUN_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO6662357.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2781..3086
                     /gene="yqjD"
                     /locus_tag="ACHKUN_004215"
     CDS             2781..3086
                     /gene="yqjD"
                     /locus_tag="ACHKUN_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO6662358.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3089..3493
                     /gene="yqjE"
                     /locus_tag="ACHKUN_004216"
     CDS             3089..3493
                     /gene="yqjE"
                     /locus_tag="ACHKUN_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6662359.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAIIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3483..3782
                     /gene="yqjK"
                     /locus_tag="ACHKUN_004217"
     CDS             3483..3782
                     /gene="yqjK"
                     /locus_tag="ACHKUN_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO6662360.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3968..4360
                     /gene="yqjF"
                     /locus_tag="ACHKUN_004218"
     CDS             3968..4360
                     /gene="yqjF"
                     /locus_tag="ACHKUN_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6662361.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4430..5416
                     /gene="yqjG"
                     /locus_tag="ACHKUN_004219"
     CDS             4430..5416
                     /gene="yqjG"
                     /locus_tag="ACHKUN_004219"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO6662362.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5709..6074
                     /gene="yhaH"
                     /locus_tag="ACHKUN_004220"
     CDS             5709..6074
                     /gene="yhaH"
                     /locus_tag="ACHKUN_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6662363.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1527 a         1516 c         1543 g         1531 t
ORIGIN      
        1 tacccatgaa taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg
       61 gaaatcactg aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatc
      121 gaacagggcg tatatctggt aggtgataaa ctgcctgcag aacgctttat tgccgatgaa
      181 aagaacgtca gccgtacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat
      241 gtcgaagtgc gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag
      301 gcggctgaca ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc
      361 cagctcatcg aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc
      421 atgaaactga tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag
      481 tgggatttac agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc
      541 atcgttgaaa aaatgtggac ccaacgtagc cataacccgt actggaaaaa actgcacgaa
      601 cacattgatt cccgtaccgt cgataactgg tgtgatgacc acgatcaaat cctcaaggcg
      661 ctgattcgca aagatcctca tgctgctaag ctggcaatgt ggcagcacct ggaaaacacc
      721 aagatcatgt tattcaacga aaccagcgac gacttcgagt tcaatgccga ccgctatctg
      781 ttcgccgaaa acccggtagt gcatctcgat acggccacca gcggcagtaa atgaaatacc
      841 tgtccgacag gcgctgcgac gcgcctgttt gtctcgtcgg taagcgaaaa gtataaagtg
      901 tcagcctgtg taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg
      961 acatgtaatt ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt
     1021 tatgtttttg aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga
     1081 tgtatcaaac ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa
     1141 aaccagcgtg gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc
     1201 tgcaagccct gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt
     1261 tgtattttgt cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt
     1321 taccgggcga cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt
     1381 acccgcaaac gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata
     1441 ttcaggggcg atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg
     1501 cgcattatca tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa
     1561 ttggtcgctt tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc
     1621 tgaataacgc gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc
     1681 tgacaactct gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc
     1741 tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc
     1801 tggtcgtgtt atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca
     1861 tgtcgcttcg ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc
     1921 tggcctggtc cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag
     1981 gcacaacgat gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt
     2041 tcaaaagtat cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga
     2101 gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc
     2161 aggacaataa cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct
     2221 tcggataact tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt
     2281 ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt
     2341 gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt
     2401 ttctctcttt gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca
     2461 aaatatcctt aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg
     2521 tctgaataaa gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga
     2581 tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc
     2641 cgaggcgaag caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga
     2701 agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac
     2761 ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt
     2821 tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag
     2881 aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc
     2941 tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg
     3001 agtatgtgcg tgaaaatccg tggaccggcg tgggcattgg cgctgcaatc ggtgtagtgc
     3061 tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg
     3121 taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac
     3181 acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt
     3241 actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct
     3301 aattatttgg gcggttgatc cgcaatatcg cctgaatgcg ataattgcca ccaccgtggt
     3361 gttgctgcta ctggcactaa ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac
     3421 cttgctgcgc catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc
     3481 ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc
     3541 aacaacggct ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg
     3601 atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga
     3661 tggcgatctg gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg
     3721 gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt
     3781 aacttccctc tggccggagc aattccggcc ttatccctca aatttcttga agatttttga
     3841 cagttttcct tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa
     3901 cgacgctaat accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga
     3961 gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat
     4021 tctgtttatt accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat
     4081 ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg attctgcttg agttcggtgg
     4141 tggtctggca atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt
     4201 tacgctgctg acggcatttt tatttcacag caactttgct gaaggcgtca actcgctgat
     4261 gttcatgaaa aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg
     4321 cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga
     4381 taaaaaacga ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact
     4441 gattgacggc gtctggcatg acacctggta cgataccaaa tctaccggcg gtaaatttca
     4501 acgttcagct tccgcatttc gtaactggct cactgccgat ggcgcacctg gccccactgg
     4561 cacaggcggt tttatcgcag agaaagatcg ttatcatctc tatgtttcac tcgcctgccc
     4621 gtgggcgcac cgcacgctga tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt
     4681 ttccgtagtg aacccgctga tgctggaaaa cggctggacc tttgatgaca gttttccggg
     4741 agcaaccgga gatacgctct atcaacatga atttctgtat cagctttatc tccacgccga
     4801 tccacactac agcggacgag ttactgttcc cgtgctgtgg gacaaaaaga accacaccat
     4861 cgtcagcaac gaatcagcag aaatcatccg catgttcaat accgcgtttg atgcgctggg
     4921 cgcaaaagcg ggtgattact acccaccagc cctgcaaacg aaaattgacg aacttaacgg
     4981 ctggatttat gacaccgtta acaacggcgt gtataaagct ggttttgcca ccagccagca
     5041 agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt
     5101 aggccagcat cgttacctga ccggcaacca gctaaccgaa gccgatattc gcctgtggac
     5161 cacgctggtc cgttttgatc cagtgtatgt gacccacttc aagtgtgata agcaccgcat
     5221 cagcgattac cttaatctgt atggcttcct gcgtgatatc taccagatgc cgggaattgc
     5281 cgaaacagtc aatttcgatc acatccgtaa tcattacttc cgcagccata agaccatcaa
     5341 ccctacgggg attatttcaa ttggtccatg gcaggatctc gatgaaccgc atggacgaga
     5401 tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct ctgataatta gctaagtgca
     5461 tgttgttcat gtcggatgcg gcgtgaacgc cttatcaaac ctacaaaaca tttatattca
     5521 atatgttgca atgaccgcgc aggccagata agcgtagcgc atctggcgat tttgcgcttg
     5581 tcatcagtct tagcgcgcct tcacagcact ttattaattc cttgaacgaa tatttactgc
     5641 catttcattc acgtctattc ttaatttgct gctttaaaaa caagtgattg agcaaaattg
     5701 aggcaaaaat ggactggtat ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg
     5761 cgcggcgcaa agagtactgg atgtttattc tggtcaacat catctttacg ttcgtgctgg
     5821 ggctgctgga taaaatgtta ggctggcaac gcgctggtgg cgaaggcatc ctgacgacaa
     5881 tctacggtat tctggtgttt ttaccgtggt gggcagttca gttccgccgc ctgcacgaca
     5941 ccgaccgctc ggcgtggtgg gcactgttgt tcttaatccc gtttatcggc tggcttatta
     6001 tcatcgtctt taactgccag gcgggtacgc caggcgaaaa ccgctttgga ccagatccga
     6061 aactggaacc ttaaaacaaa tctgctcgcc ggatgtctga ctcatccggc ttttatc
//
LOCUS       DAZFRJ010000230         6069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000230 DAZFRJ010000000
VERSION     DAZFRJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.232"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..505)
                     /locus_tag="ACHKUN_004221"
                     /pseudo
     CDS             complement(1..505)
                     /locus_tag="ACHKUN_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(510..674)
                     /locus_tag="ACHKUN_004222"
     CDS             complement(510..674)
                     /locus_tag="ACHKUN_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662364.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(671..1404)
                     /locus_tag="ACHKUN_004223"
                     /pseudo
     CDS             complement(671..1404)
                     /locus_tag="ACHKUN_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1438..1899)
                     /locus_tag="ACHKUN_004224"
     CDS             complement(1438..1899)
                     /locus_tag="ACHKUN_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6662365.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1892..2932)
                     /locus_tag="ACHKUN_004225"
     CDS             complement(1892..2932)
                     /locus_tag="ACHKUN_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO6662366.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2904..3455)
                     /locus_tag="ACHKUN_004226"
     CDS             complement(2904..3455)
                     /locus_tag="ACHKUN_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6662367.1"
                     /translation="MKITTEQVCEALDTCVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3439..3666)
                     /locus_tag="ACHKUN_004227"
     CDS             complement(3439..3666)
                     /locus_tag="ACHKUN_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6662368.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3743..4150
                     /locus_tag="ACHKUN_004228"
     CDS             3743..4150
                     /locus_tag="ACHKUN_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662369.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4415..4714
                     /locus_tag="ACHKUN_004229"
     CDS             4415..4714
                     /locus_tag="ACHKUN_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662370.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4787..5005
                     /gene="ydfC"
                     /locus_tag="ACHKUN_004230"
     CDS             4787..5005
                     /gene="ydfC"
                     /locus_tag="ACHKUN_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6662371.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5028..5435
                     /locus_tag="ACHKUN_004231"
     CDS             5028..5435
                     /locus_tag="ACHKUN_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662372.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5413..5646)
                     /locus_tag="ACHKUN_004232"
     CDS             complement(5413..5646)
                     /locus_tag="ACHKUN_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662373.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1493 a         1582 c         1348 g         1646 t
ORIGIN      
        1 catcctgttt ggaatagcca gcctttaatg gataactatt tctgacaatg caatgagtat
       61 aatcaagtcc atcttccact gagaattaga ggcggcatgc tttttccggc tcttgccgga
      121 tatccgtaat tgtccataat ctgcagattt ataccttctg catgacctgt cagcgaaaat
      181 ttgtccggtg tttctacggg aatgacatca aaagttacac gcactcgcgt taccgtgtag
      241 acctactttc ctgcacttgc aagatcacag tggtgtaacc gtaacaggaa tttattctct
      301 ggaccggcag taaatccctg agtggcgtgg ttcccatatc aatttcccgc caggcagcct
      361 ccattgccag cgtacaggct ggagccatga cctgcccttt aaatctggcc cgaccatccc
      421 accggacgtg ttcttctccc ctgaacttag gtacagtcat ctccagtggc acaaaagtgt
      481 cagcgccata atttttgacc gttatcgcgc tacggatatt ttgttgactg gtgaaaatca
      541 ccccgcagaa tcaggcttat tcccttaacc tgggctttca tcctgaccgc cgcctcacta
      601 cgaccaatca gactgccgat gcattttacc ttcattgtta gtatcatgat ttcaggcctg
      661 caccatccgc tcattgcccg gacttccgac aaatcccggc aaccatatcc cggtgcttgt
      721 tcagctcccg cagcgcggcg cagactcgct cccacttctg gacatgattc ttcgccctac
      781 gcagttcgcg atttgccata tgcagcgatg gcaaaatcaa atcatcttct cgcgttgcag
      841 taaacgatgg cagcgactgc acaatgtccg ccacagtttc tgttttaata tcttcctgtg
      901 ttgcatcttt ctgtaccggt aacgaacacc ggctggctga ggaaaggctt taccatcggt
      961 ttccgctacc gattcaactt tcggctctgc tggtaaatta tcacctggta tgcagtaacg
     1021 aattttaccg ttctggttta cgcgaatcag acgacctttg ctgattgcca ttgccagcgt
     1081 tgaagcaact ttgcgggatg ttgtaccgaa cagcgtagcc agttcatccg ccgtttgtgg
     1141 tccgcgttgt tcaatcgtcg cggttaaatc gcactctgag attttcgctt ctgttgccgt
     1201 ggtggtttct tctggcgctg gctgttcctg ctgaacgttg ttatcagcca cacgccaggt
     1261 gtatacgctt ttatcaacga agccagcctt tttcagttcc cacagctcgt tcagtacttc
     1321 ttcacgactg atatcaagtc gcgcagccag ctctaccgac gtggcttttc ccatcgcttt
     1381 cagtgcgtca aaaacagtct ccataaattt cctcccggta aaaaatcact tctcaactca
     1441 aacaaaacca gccgctttcc ggcgttcata ttcctgtttc agcaactcaa ttggcgttgg
     1501 ccccgacggg cgtttggggg ccgccagttg tcgccggact ggcggaacgc tcaggccgtt
     1561 actaacatgc tttgcccatt tcgtcagttg ccgttctgca agccgtttta attccccttc
     1621 ggtcatctgg cgctcaatcc cctttgaacg catctcgagg caaatgtgat acagcacagg
     1681 ctgagaccac gggtacttat cacttccgtc gtatcgccag gactcattgc gccagcggcg
     1741 gtactcctcc atcacagcat ccaccgtcag accgaatgga ttggctccgc ttttcgaaat
     1801 cagtgccaca aactcagcca ggtccggagg ccatgtttca cccgcccggc agcggtccat
     1861 gcactggcgg cagacctgcc ggatttgctg ctcagtcatc gcgccaatct gtgcaatcca
     1921 gagcttcgaa ggtgcggccc cgttcttctg gatccagcgg ttcgaataaa ccacccccat
     1981 gagttcccac agcttccagg ccgtttccgt cgctgataaa tccgttttca cgttcccact
     2041 gctcacgtgc tgcccgaatt tcctgaactg cccgtgatgc ggtgccacct ggtgctgctg
     2101 catggtttac ccctttgctg actggtttaa cctgcgccct gacgtgattt acgtgacggg
     2161 cgaatttctg ctcccactga atctgcgtaa acactttccc ctccgctgcc cagtagtccc
     2221 ggaaggcggc aagttcagca ggtgtaaatt ctgtctccgg caaagccatc ccccacaacg
     2281 cagcccgtcg tcgaaaatcc cgtgacggat accagctatc gctcatcggg aattttccga
     2341 tgggttcgct caggccatac aggaatacag ggggggctgc ctgtaacgac aaaacttcct
     2401 gctcactggt cggagcactc tcgcgtgcgt tatgtgtggg gtttagatct ttgggttcct
     2461 ttgggttccg tgatccgttt ttgggtgtct ttgatggaaa atttgggtgt ctttggttat
     2521 tttccatgca gctaagagtt ctgtttttgg gtctgttttg tgctgaaaca taaccatttt
     2581 cggtactgtt tttattaaca gcaccaattt tacccacctt taaagactcc cgtttttggg
     2641 tgtattcagg ctcggcaaca ctttcttcta caccgataag tcggtacacc acaatttgct
     2701 ttgttctgcc ttttctctca ccggtatcaa caattaaccc aatctccatc aggtgtcgta
     2761 agctgtcctg cacagtcttt ttgttcagtt ccgttacttc tgccagtgca gatacagacg
     2821 ggtatgcaca caaatcggca ccgcacatat cagcaagcca ggtcaatact gacttactgg
     2881 atgaactgcc ggttttcacc tttttagccc atcgtagtgc atcgatactc atacgaaccc
     2941 ctggcagaca tttgtttatc tgcaaagtaa tattggtatt gctgacgata cgcgtgcttg
     3001 aaagcaatag ctttttctat aagctcgtca gtctcacgtt ccacaacaac tggatccgca
     3061 aaaagcagcc cggactccac cacatcgcca tattctttgt ttaacccggc gatcatgtac
     3121 gtaatgcttt ttccgtcagt aatttcacaa tacaatctga aatcgctgat ccggatagcc
     3181 tccataattg ccggaatcag cgccgtgaat ttgtcacgct tatctctggt gtcgatagct
     3241 ttccagcgtt cgaatatctt cacccggtta acgcccagcg cccgttgatc aacctcgcca
     3301 tcattaaacg tgacgcgttg aacatcgatg ttcgggcgtt ctttcagagc ccagaatgct
     3361 tccgtgatta atatcgtcgc ctgctcctgt gtcattcctg gtcggcatac acaggtatcc
     3421 agagcctcac aaacctgttc agtggtgatt ttcattgttc aaccgccccg cccgctttgc
     3481 cttacgatat tcgtcataaa ctttggggtc gtactgaagt tccccgccgg atgcctcttg
     3541 caggcgcatc gcgcgacctt caggaaccag ttccccccat gcagcaacgc ttgccagcct
     3601 aactcctgcg acattggcaa gctttgtttt gctgccaaaa aacgctatag catcaatttt
     3661 caacatatcg aaccccttag attttcctaa ggaaactaga tcgtagagaa acctaagtca
     3721 agaaaaatta gaattaccta atatgaaaaa cgaaaccttc ggtgctcgcc tcttatacag
     3781 gcgaaaaaaa ttaaaactgt ctcaggccgc attaggtaag ctggtcaaag tggctcacgt
     3841 aacaatttct caatgggaaa gagatgaaac acagccagcg gggaagagat tattcgcact
     3901 gagccaggcg cttcagtgct cgccgacttg gcttcttttt ggagatgaag ataagcaacc
     3961 aggcgaaccg atcccagata atcagccagt caatctgaca gaagatcaaa aagagttgct
     4021 tcaactgttc gacgcactgc ctgagtcaga gcaaaaggct ctgttgtcag agatgcgtgc
     4081 tcgagttgag aatttcaaca aactttttga agaactactc aaagctcgca aaagaagcgc
     4141 aaataaataa cccccctttt tttcgcaact ctctgtaata aaaagcacaa actttcaaat
     4201 acttgtgttt tttacatcaa gaagcttagg tttttctaca caaaaagctt gaccataact
     4261 cttaggctat tctaaattct actcatcaag acaccgcacg gtgttctcag caaacagttc
     4321 cgctacccgg cgttaagggg aagcagagga tttctcagtg ggcgaagtca aacatcagaa
     4381 tggaaggcgt ccagggatca gcaaagaaac agcgatggcg ctttatattg atatcagcgc
     4441 cattgccgga caggtaagag ttatcagagc ggtaactaag cggtatgcgc ctttacttca
     4501 gaaagtctct ggtgagtgca ccgaagatat tgtcaacgat ttcgtcatcg aactgcgagg
     4561 actcatcttc agttacaagg tgaccacaat ttttgcagat ggctcccgcg aaactgtcag
     4621 agccctgcgg cttaaaggat gtgtcaaaga cttagccacc acattctggg caagaaaact
     4681 tgattgtatt cataaccaat ttcctctcga gtaacagacc cctcagagga taccacctcg
     4741 cctgacgtgg ttaaaaagca ggcaacgcta accacaagga gccgacatgc agaaacgaga
     4801 ccccgtcatc atcgcgccag actataccga tgatgaactt tatgagtgga tgcaccagaa
     4861 aattaaggcg gtgcaggacc tgaaatgggc caatgaagcc agggctaagc aggctgaaaa
     4921 tctgtccgct ctggagcagg atatcaccaa tctggaaaaa gcagcggcat taagcattgc
     4981 cagaatgatt acatacccac gttaatagct aaccaacgag gctaataatg gaatttaaag
     5041 atttaccaac gccattacag gaaatggcat cgaatatagt tcgttcacaa ctggctactc
     5101 ttgaccttag taccgcagaa aaagaaacca tcgataatat ggttcgtaat gtgcgcaatg
     5161 ctttttctgg gctatatggt tctgataatc aaaagcagga aagcgatgtt aataaacggg
     5221 taatttctgt ttgcgtgaat ggccatgttc tttcatcaat caaaacagaa acggcgacag
     5281 tcttcgattg cctttgcatt gtacagagcc ttgttgatgc cctgtttcgt tcagtgaatt
     5341 tagaaaatga tgcaaatctg cgagggcgca taatagcaca tccatatgca catactttag
     5401 gctctgtgga tatcaaagat cccacaaatc tttaatgaaa tagttaacgc gaattgtact
     5461 tgctctttcg gttgctttca gaatacgcgt tgaaactgct ggcggtaatt tggtattcca
     5521 tttattaaaa tcatgcccgg gaaagtactc ttcgaaaata cttttaactg cagactcgcc
     5581 tattgaaatg ctgcttacca tgcgattttg ataaaggcat ttagcaataa gagttgattt
     5641 taacattcac cctcctgagg gttggtaatt aaggagttct ccacgggtga agtggagtgc
     5701 gtgcgccgga cacgggtgaa catccggcac tgacagttta ctgaaaggat atttctctga
     5761 aaagtcagac cataacgcga aagcgcacgg cgaggtagct ggttcataga tagcctgtcg
     5821 ttaaattttc gtcgaccgtg cgcttccggt tgtggcaatc cgcgaaatgg cgcggcggta
     5881 agtatggagg ggttattcct tccccgttga ggacaccggg ttgtcaggtt gaccatacgc
     5941 ttaagtgaca accccgctgc aacgccctct gttatcaatt ttctggtgac gtttggcggt
     6001 atcagtttta ctccgtgact gctctgccgc cctttttaaa gtgaattttg tgatgtggtg
     6061 aatgcggct
//
LOCUS       DAZFRJ010000231         6041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000231 DAZFRJ010000000
VERSION     DAZFRJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.233"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(465..602)
                     /locus_tag="ACHKUN_004233"
                     /pseudo
     CDS             complement(465..602)
                     /locus_tag="ACHKUN_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(604..1161)
                     /locus_tag="ACHKUN_004234"
     CDS             complement(604..1161)
                     /locus_tag="ACHKUN_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6662374.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            complement(1158..1715)
                     /locus_tag="ACHKUN_004235"
     CDS             complement(1158..1715)
                     /locus_tag="ACHKUN_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662375.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            complement(1712..1876)
                     /locus_tag="ACHKUN_004236"
     CDS             complement(1712..1876)
                     /locus_tag="ACHKUN_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO6662376.1"
                     /translation="MRGLAYNPGILPAEMIIRQRAKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(1887..2180)
                     /locus_tag="ACHKUN_004237"
     CDS             complement(1887..2180)
                     /locus_tag="ACHKUN_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO6662377.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(2204..2587)
                     /locus_tag="ACHKUN_004238"
     CDS             complement(2204..2587)
                     /locus_tag="ACHKUN_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662378.1"
                     /translation="MAHSITVRLNKPAREFQTGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNGPQQQSSNQQNTPVYDDSIPF"
     gene            complement(2587..3192)
                     /locus_tag="ACHKUN_004239"
     CDS             complement(2587..3192)
                     /locus_tag="ACHKUN_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO6662379.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(3294..3452)
                     /locus_tag="ACHKUN_004240"
     CDS             complement(3294..3452)
                     /locus_tag="ACHKUN_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662380.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            complement(3449..3601)
                     /gene="kil"
                     /locus_tag="ACHKUN_004241"
     CDS             complement(3449..3601)
                     /gene="kil"
                     /locus_tag="ACHKUN_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO6662381.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            complement(3586..3717)
                     /locus_tag="ACHKUN_004242"
     CDS             complement(3586..3717)
                     /locus_tag="ACHKUN_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO6662382.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(3742..4710)
                     /locus_tag="ACHKUN_004243"
                     /pseudo
     CDS             complement(3742..4710)
                     /locus_tag="ACHKUN_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            4966..5505
                     /locus_tag="ACHKUN_004244"
     CDS             4966..5505
                     /locus_tag="ACHKUN_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO6662383.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            complement(5529..5801)
                     /locus_tag="ACHKUN_004245"
     CDS             complement(5529..5801)
                     /locus_tag="ACHKUN_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO6662384.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(5779..5998)
                     /locus_tag="ACHKUN_004246"
                     /pseudo
     CDS             complement(5779..5998)
                     /locus_tag="ACHKUN_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1346 a         1471 c         1296 g         1928 t
ORIGIN      
        1 ttaggcgcag ctgagacagc aacattccag aactcagaaa acatattgta tgcaccagat
       61 agattggaag tagcataccc tccaagctca cttctaatct gaacggctct catcatttcc
      121 ggggttaact ccttcggcac cataacccaa ccatccggag ttaccggaga gttgccaccg
      181 ggaatatttt ccggaatatt ttgttgtgcg ttttgtggtt gttctgattt accctgaagc
      241 atggcggcgc ggcaggcgtt ccagccagct gttcgcccaa gcgcgtaaac ttcagatgga
      301 tcaagataat caatgtcatg cccgtcctca tcgtcgttct caggtaatgc agcaggtact
      361 accggtactg gcggagcggc gtagacctca ataattccat tatcaatagg ccattctcca
      421 tccttgaggt agtcacttgt gccgtcaact tgctgttctg caatgtggaa tgcacctatt
      481 ggttttgctt ccagcgatgc cagagcaatc cgtgccagtt ccatttgttc gccactagta
      541 agtccgttat caagcgggga tttaatgaac aattcgatac gttctttagt gatagtgctc
      601 atatcactct cctttgatgc gaatgccagc tgcgcttgct gattcttcat atgcgcgttt
      661 agcagcgtta aggattgctg ccagtggcgt atagccgcca tccattcgga ttgtgttgtg
      721 gatgccagcc attgtgtcgc gcaatttgct gtggctagca gacagttctg ctatgcactt
      781 ctctgcggca tcggctcgat ctttttcacg ctggaacatt ttgtgagtgc ggtctatagc
      841 ttcgattcgt gccgctgatg tcttctgcac ggcttcaagc tcaacacgca gcttcccaac
      901 cgtaagagca atatcctcgt tcttctggtc gcggcgtttg atgtattgct ggtttctttc
      961 ccgttcatcc agtagtgcca gcacggtttc tggcccggtc agaaatttga aggcgttgag
     1021 cgcatcaata tccacaccgt aatccttaag ttcctgttca gttaacaaat catcatcaac
     1081 tggcaacatt aacaggcgtt ccattgccgg aattgcacgc tctgccgcct cacgcagtgc
     1141 ctgatagtca atcttgctca ttggatgact cctttacgaa gctgttcggc gatatcttcg
     1201 agaacgccat cagagaatga acggtcaaaa tcgccttccg gcgcattggc cataaactca
     1261 gtagaggtaa gaatcatccg ggcaatatca gcggcgttct tcgcagtatc atcaataaaa
     1321 ccagcttccc aggcagccag cattctgttc gccacaaagt aagcgccctc cttgcgtgct
     1381 tcagtcttca cttcagccag aaaagcatcg gtggctgggg tttcagtaat atcatcttcc
     1441 cattcgctaa actcctcacg acaaaagtca ttaaattcct tctcagattg cttaagcgag
     1501 gaattttcag cagccatctt cgcgcattta gcctcaaggt tatcaatcgt gattccagca
     1561 gaacgacact cccgcaacgc cgtttctagt tttgattcaa gttcaccgaa cttacggaca
     1621 agatattcat cgtttgtttc gtcaaccttt aaatcacttg ggatgcattt acctttcaga
     1681 aaaccatcca tctcaattag tgtcatttgt ttcatttctt cccactccgc cacatcgcat
     1741 tcagatattt gttgtcatta acagaaccga aactctttct cttaagcaat tcctctctcg
     1801 atggcattgg ctttgcgcgt tggcgaataa tcatttctgc cggaagaatg ccgggattgt
     1861 atgcaagtcc tctcatggta aattcctcag tcattactga tagcgccata gcgtgagcgg
     1921 taattacgca ggcgcgggtc aatttcaggg aagtgggtat atgtggcttt gcggaatggt
     1981 cggattgatg tctggtaaat tcgctcgcgt tcttctttct ctgcaagcca tatacaatgg
     2041 cgaaattcct tttcctcttt cgtttcctgc ggtagcgaca ttatccgatc gtagtttttt
     2101 ctgaatttat ccagcacctc cgatacggaa ttgccggaac agcggcgcgc gtcatccgca
     2161 ccatacagag gcgctggcat aattaaatcc ttatttttct aaatcagaat gggatggaat
     2221 cgtcgtatac aggagtgttc tgctggttac tactttgctg ctgcgggcca tttcctgaag
     2281 ctgcaaatcc aatctttgca ttcagtaatt caagagtgat tgattgacca ttttgcccct
     2341 gataaacatc aaccctgatg ttttctccgg taatttccac aatgccacct tcaacaagaa
     2401 cactacggta gtaatccgct tgcgctcccg gcttggcaaa tacaacggcg ctgtagtttg
     2461 tccattcttt cttttttgtc tggcgatcgt aatactgaac gccagcacgg atgttgaatc
     2521 cgatattttc cccggtctga aactctcttg cgggcttgtt tagtcttaca gtaatcgaat
     2581 gtgccattaa gcagcagctc cttctaattc gtctcgtctg atgttgtaaa cgtcctgcgc
     2641 tttgtgctgc tccggtgtgc cttcgagcat cttccacgct ttggcgaacg cctgtttaag
     2701 ctcttctacg gtgtttttct gcattgctgc gtcagtgaat gcttttagaa cctgttcagg
     2761 tgtaggtgat ggttttgatt gctttgctgc tgcgttctgc tgatgtttat gctcgtcggt
     2821 atctgcatct ttcgcatcat caatgccgaa taaaccattg aggcaatact tgcgtgcata
     2881 agagcttgta gctcccgtaa cttgtgcaga atccattcct ttcttgcttt cttcctctcg
     2941 tgcaagagcg gttgccgtat gactgttttc gccatcggta atagttgccg tggctttcac
     3001 ataataccga tcaccaatca acacaacttc atcgctgatt gataaaaaca ggccattcag
     3061 taacggctta acgccttcaa gaatatcttc gcagcttctg tatttatatt tgccgaatga
     3121 gttgtactga ttctttggcg cgttcagatt ctcctgaata gctgccagtc ttgcgtaaaa
     3181 ttctttgctc atatgattgt tctcagaatg gacacggccc aaggaaataa cgctgattta
     3241 atacttcgac tcgggacaaa ttaaggcata cccgcattcc ttcgcggtca ccattatggc
     3301 gataccagag agctttctgc gtgtacatgc gcctctgtaa cttgctctcc ttcactgtgg
     3361 ttgcaagtga catgaatatc tccttcgtta ccgattaatt ctttcatctg acgaatgaat
     3421 tcttcgtctg accagttatc tgtaaaactc atttcctgcg ataacacgga aggttgatag
     3481 ctgatttcat cgctttattt gcttcaagcc acatttttga atcaccaata aatcgggcta
     3541 ttactgcctt gttctgtgca gcacgaagca tctggtgatt gatggctatt tcattgcgca
     3601 taataagacc tcaactcttt tccatccgtc acgtaattta cgggtgattc gttcaagtaa
     3661 agattcattt aattggaagg cacccatgcg agcgcctccc gcgattgcgt aaatcatggg
     3721 tggttcctta tgttggtttt attagtaggt tatttttgtt gcgaataatt cgccttttac
     3781 gatggctgtt atgatatttt tagcaacatc ttctgatgcg ccaaccttga taaggtcagc
     3841 aagtattttg ttatttactt ctttccggtg agctttatcc tttgctctac gctcttcttc
     3901 gtccttgatt cttttttctt ctgctattct ggcttgctct tttgcttcag cctcgcgcag
     3961 gattcgttct gcctcctcct gtgctttacg gcgttctgct tcaattgccg catgcttttc
     4021 tctttcagct cgttctgctg cctcttttgc ttcgcgctgt gctcgttgct cggcttcaat
     4081 gcgttcacgc tctgcacgtt cctctgcggc cttagcttct gcttctcgcc ttgctgctgc
     4141 ttcaatttcg gcttttgcct ttgcttcggc ttcagctctg gctttctctt cagcttctct
     4201 ttttaagcgt tcttcatgct ctcgcttttc ctgctccgct ttgagtcttg cctcttctct
     4261 ttggcggtca aattcgcgat ccatcaaaat cgctatttca tggtcagact caatttgctt
     4321 tgcgagagct tcagctgctg ccttagcttc ttcttcggct ttaatccgcg cctgttcttc
     4381 ctcataatca gtaagaggct ggcgcgcctt ggctttcagt tcatcaaggc gatcgcgcac
     4441 tgtcttgcgg ttagcatcaa ttagctttgg aatttccttc agttcagcaa caaggtcttt
     4501 gccaagacca tcgagatatg ttttcgtctg cgcaacttta tacgccagat aagcgatctc
     4561 ctttctgccc tttgccgttg tgatatcagg cacaaaggac ataacttcac gttcaacctt
     4621 ttgaagaatt tcttcaatct ggtcggcaga ctaaattaca gtcattgcat ttgctttttc
     4681 aataacaact aaatctgtta cttcactcat atatcctccg tcaaaaaaat tgccctcaca
     4741 ctggagggca aagaagattt ccaataatca gaacaagtcg gctcctgttt agttacgagc
     4801 gacattgctc cgtgtattca ctcgttggaa tgaatacaca gtgcagtgtt tattcgtatg
     4861 cctgtctttt aaccacatca ggctcggtgg ttctcgtgta cccctacagc gagaaatcgg
     4921 ataaactcta ttcaccccta cagagagaat gatggagatt caccgatgag taactggtgg
     4981 caggaactat tacgtttctt cctgcgtggt cttacgctac aacagttaat tcatatgctt
     5041 attattttaa ttgccttgat aataatcacc ccagcatcaa ttaaagagtg ggtagatata
     5101 aggaaccccg aaatacttcc agatcactgg atgtattacg caatgctttt gtgtatcagt
     5161 tatgttctga acagggggat ggagtttata tttctggcat cttcagacag atataaaaaa
     5221 tatctcagta agagagatga ggctaaagta attgtggaga ctgagcgcct attcaattct
     5281 ctgagtattc aggaaaaaga ggttttagca tttgctgtta tggcaaataa caaaatcgta
     5341 cttaagcacg gcgatccggt cgctttatct cttatgagaa aaggccttct ccatcgctca
     5401 ggtgtgactt acagcgcgtc aggtaaagag aaatttgtta tacctgacgt ctggttccat
     5461 gagtgttata tgcgctttgc tggtaaagct gatgagctaa tttagttcct ctacggcggg
     5521 ggggatcgtc acctcgctgt cagttgtttt gatttccggt agcctgccgc gtaaatggct
     5581 acgtttggaa gacaagttga accttcatat tttctggtca acgttgtcag agttataact
     5641 tctgctctca ttgctggttt gcgcttgcat tgcaagacca ctcgtgaagg ggttggcctg
     5701 tgtagcttgt cggagctaat cgcctcctga ctttgcaggt ttgcgcgacg agctctacgg
     5761 cgagaagctg cggtgccttt aaattctgtt tttctggaca tagattcctc ccgaataaac
     5821 tttggcgatg caatctcgaa gctcctcctg agacggttac ttcggcattg catcccacag
     5881 cttatgtggt tgggtgatct ggcttttcag ccacgtagtc gagtgttcga cgttgtttaa
     5941 agagcctgcc agtctgctcc gtttggctta cagcgtcctg ctgatgagtt gactttatcg
     6001 aaatgataaa ctgatggcaa tagcaaaatg ataaattgtt t
//
LOCUS       DAZFRJ010000232         6010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000232 DAZFRJ010000000
VERSION     DAZFRJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.234"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..394
                     /locus_tag="ACHKUN_004247"
     CDS             38..394
                     /locus_tag="ACHKUN_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO6662385.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            398..1525
                     /gene="dapE"
                     /locus_tag="ACHKUN_004248"
     CDS             398..1525
                     /gene="dapE"
                     /locus_tag="ACHKUN_004248"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO6662386.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            1553..1753
                     /locus_tag="ACHKUN_004249"
     CDS             1553..1753
                     /locus_tag="ACHKUN_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO6662387.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1863..2561)
                     /gene="ypfH"
                     /locus_tag="ACHKUN_004250"
     CDS             complement(1863..2561)
                     /gene="ypfH"
                     /locus_tag="ACHKUN_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6662388.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(2635..4650)
                     /gene="tmcA"
                     /locus_tag="ACHKUN_004251"
     CDS             complement(2635..4650)
                     /gene="tmcA"
                     /locus_tag="ACHKUN_004251"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO6662389.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            complement(4665..5528)
                     /locus_tag="ACHKUN_004252"
     CDS             complement(4665..5528)
                     /locus_tag="ACHKUN_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO6662390.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5675..>6010)
                     /gene="vapC"
                     /locus_tag="ACHKUN_004253"
     CDS             complement(5675..>6010)
                     /gene="vapC"
                     /locus_tag="ACHKUN_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HFO6662391.1"
                     /translation="VRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVS
                     RLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERV
                     AGIRLEDWS"
BASE COUNT         1459 a         1651 c         1513 g         1387 t
ORIGIN      
        1 tgagtattta ttctcataaa tcgaaaaagg attcattatg gttacacttt acggcatcaa
       61 aaattgtgac accattaaaa aggctcgccg ttggctggaa gccaataaca tcgactatcg
      121 ttttcatgat taccgcgtcg atgggctgga cagcgaatta ttgaacggtt ttatcaacga
      181 attaggctgg gaagcgttac tcaacacccg tggtacaacc tggcgtaaac tggacgaaac
      241 cacccgcaat aaaatcaccg atgcggcctc tgcggcggca ttaatgactg aaatgcctgc
      301 aattatcaaa cgtccattgc tctgcgcgcc tggtaagcct atgctgctgg gtttcagtga
      361 ttccagttat cagcaatttt tccatgaggt gtagtctatg tcgtgcccgg ttattgagct
      421 gacacaacag cttattcgcc gcccttccct gagtcctgat gacgcaggat gccaggcttt
      481 gttgattgaa cgtttgcagg cgatcggttt taccgttgaa cgcatggact ttgccgatac
      541 gcagaatttt tgggcatggc gtgggcaagg tgaaacgtta gcctttgccg ggcataccga
      601 cgtggtgccg cctggcgacg ccgatcgttg gatcaatccg ccgtttgaac ccaccattcg
      661 tgatggcatg ttattcgggc gcggtgcggc agatatgaaa ggctcgctgg cggcgatggt
      721 ggtagcggca gaacgctttg tcgcacaaca tcccaaccat acggggcgac tggcatttct
      781 gatcacctct gatgaagaag ccagtgcaca caacggtacg gtaaaagtcg tcgaagcgtt
      841 aatggcacgt aatgagcgtc tcgattactg cctggtcggc gaaccgtcga gtatcgaagt
      901 ggtaggtgat gtggtgaaaa atggtcgtcg cggatcgtta acctgcaacc tcaccattca
      961 tggcgttcag gggcatgttg cctacccgca tctggctgac aatccggtac atcgcgcagc
     1021 acctttcctt aatgaattag tggctattga gtgggatcag ggcaatgaat tcttcccggc
     1081 gaccagtatg cagattgcca atattcaggc gggaacgggc agtaacaacg ttattccggg
     1141 tgaactgttt gtgcagttta acttccgctt cagcaccgaa ctgactgatg agatgatcaa
     1201 agcacaggtg cttgccctgc ttgaaaaaca tcaactgcgt tatacggtgg actggtggct
     1261 ttccgggcag ccatttttga ccgcgcgcgg taaactggtg gatgcggtcg ttaacgcggt
     1321 tgagcactat aatgaaatta aaccgcagct actgaccaca ggcggaacgt ccgacgggcg
     1381 ctttattgct cgcatggggg cgcaggtggt ggaactcggg ccggtcaatg ccactattca
     1441 taaaattaat gaatgtgtga acgctgccaa cctgcagcta cttgcccgta tgtatcaacg
     1501 tatcatggaa cagctcgtcg cctgatgagt ggttctgcaa gaggaaataa gcatggactg
     1561 gctggctaaa tattggtgga ttctggtgat tgtctttttg gtaggcgtcc tgctgaacgt
     1621 gattaaagat ctcaagcgcg tcgaccataa gaaatttctc gccaacaagc cggagcttcc
     1681 cccgcatcgt gatttcaacg ataagtggga cgatgacgac gactggccga aaaaggatca
     1741 accgaagaag taagctgagt gctcaaccgt aggccggata aggcgttaac gccgcatccg
     1801 gcaatggtga acgatgcctg atgcgacgct cacgcgtctt atcaggccaa gtaatctgtc
     1861 ttttacatca tttcaatcac atcatcatcg ccaggcttac cgccacttag cgcttcgtcg
     1921 aaataatgtt tcggaatggt ataacgcaag tgatccaggg caaattgcat actgcgattg
     1981 tcgatcgcat ggcctaaatc ctccacgata tccagcgtca cgtcaccacc ggcactgatt
     2041 aacgcttcct gcgccgcaac tgcatgcgcc agatcgataa ccggatcttc accaccatga
     2101 atcaagtgaa ttgtcgtggc tgtagacgcg gtctccggca ggctcgcata gcgtccgtta
     2161 aaggcaatga cgcgcgaagc aaggcccggt tcggctttaa tgctctccag cgccataatc
     2221 gcgccttgtg agaaaccaat tagcgccgtg gcgttagccc ctactccgct ctgtttctgc
     2281 cagtagcgta ccgtttcaat aaacgtcggc atgatggcat ccacacgcgc ctggcgatta
     2341 tcttccgtaa taccctgcac tgaaaaccac tgacgccccg ccggattacc gctcggctcc
     2401 gcgccaccga cactcaccac cagcgcatca ggaaacagcg gtgcaaacca gctacctatt
     2461 tcccccattg ccaccgggtt atccccgaca ccgtggaaaa gcagtaacaa ctgttgcgca
     2521 ggtttatccg ggctttgaac aacaaaatgg tcatgtttca tggcgaactc cttaactgat
     2581 gtcattaatt ttacgccgct gactcacaat gaccatggga gaaaattgaa ggagtcagtg
     2641 aaaaaattgc cattgcgtta tgcgatcgcg cagacgctca gtgcgaacat cattaagtgc
     2701 gaacagcgcc tgcgcggcct cttcccgctg acggaccagt aacatcttgc ggcctgaaag
     2761 tttaagtgtg gtacataact gcgcgtcgct gacgtttttc tgtaaacgcc cacgcagcgc
     2821 cggtaatgcc agttcactgg tttgtagcag acgcaataag caacctaacg atgttaatag
     2881 cggacgatga gcgaaagcaa aaccggccag ttcaagccag tcgtcgtcag aaaggacggc
     2941 atcgtttagt ggatcaacag gaagcgtttc gccattccac tttgcgagag cttgcgcatc
     3001 gcgacgtaaa cggtaatgct cacgttcagc cagttgttta cccgcattac tcatcggtaa
     3061 cagcgccatc gccgtatagc aaccgctgct ggcttcacga tgattaccca tccgcaccag
     3121 cacaaaaccg cagcggtgcc agaaacgcca taactccccg gtgtaaccaa aactcaccga
     3181 aagatagtcg aggtcacgcg tatattgcaa agcaccagca ataagttgct gccctgtgcc
     3241 ttcccgctga cgcgccggat gaactgctat ccggctgacc cgccgtccac gcaatgtcgc
     3301 tgccagtgga ttgctaccgt gcgccgccag cgactgggcc accagattac cccgcgggcg
     3361 acgaaaacct gcccataccg cctgactgag ttgttgagat aatcccccct catccaccag
     3421 ccacagcgct ccggcaatct cgttttcgcc agccgcctgt aaaaaatgtt gccctggtgc
     3481 atccatcatc cggcgtaaat ccagcggcga agtccggtag tgcgcaccag acaataactg
     3541 ataaaccttt aacggcgttt ctggctcgct tcgccataac gtttgttcaa atgcggaaat
     3601 gacgatattg ccttgtggtg tatgggtgaa gttttcatcg tcaaaaacca gtgcctcact
     3661 aaccattttt tccagcgggc atccctgtgc ccagcggatc ggctgttgca gttcaaaacg
     3721 gtgtaaatgc ggaaagcgag cgcaaaattt cagcaaaaaa ccacgtccgg tgccttcgta
     3781 gccctgcacc gtagtggtta acaacgttcg aggaaaacgc gataccagtt gatgcaacaa
     3841 cggcgcaggt atggctgcgg cttcgtcgac caccagccag tcggcttgct catcgctggc
     3901 taacaaggca tccggtgcaa taaagcgaaa cttctcgccc gcaaattgtg ccagtacatc
     3961 cgttgccgct tttgcgggcg cggtgacaat cgcactgccc gcaatacgag aaatgagttg
     4021 ccctgccaac gccgacttac cgcgcccacg cgcagccgtt accgctgcca cgcccggagg
     4081 catggtcagt agctgctgta agagttgctg ttgttctggt tgtggtgcgc cggtcgcggg
     4141 gtgccagtca gtacggggag taaaatgcgc caacgagaac ggctggtttt gccgccagag
     4201 gatagcgtcg ttatccgccg taagtacgcg tttgagatgc tggacaaaat gcggcgtcgc
     4261 aatagggtca gggcaatcac tccagcgcag cgagtcggca tcaggttggt tttcccactc
     4321 ttcccataca gggagtaaca aaaccagcca gcttcccgct ttcaacgttc cgctaagtgc
     4381 tgcaaaggca gcggcatcaa agccgtggcg ggcgtcgaat accgcatgcc ggaactcgcg
     4441 cccaagtaaa gtttgtagcg ccgagggtga acagtggttt tcagcatctg gctgcggcga
     4501 aatccacagc cagtcgccag gtaaggcatc acgcaactta agcgcatgat caaaacacca
     4561 accctcttcc ccgctcaaca ccagcaagcg gcggatccct tcacgtttca tttgcgctgt
     4621 taatgtgtga agcgcagtca gttcagccat ccctgccccg gaagttaaat gcttttacca
     4681 aaagtattgc attgtgccgg atcgccgctg tcgaaaccac gtttaaacca gctgtagcgt
     4741 tgctgagaag tgccatgtgt gaaactatct ggtactactc gcccctgact ttgctgttgt
     4801 aaacggtcat cgccgatggc ctgcgccgcg ttcagcgcct cttccagatc gccggtttcc
     4861 agaacgcctt gctgctgcat actatgcccc cagacaccgg caaaacagtc ggcctggagt
     4921 tccatacgca cagataagcg gttcacttcc gcctgcgtcg cgttttgttg cagttgacga
     4981 actttcggct cgatgcctaa cagtttctgc acatgatgac cgacttcatg ggcgataacg
     5041 tacccctggg caaaatcgcc atccgcgcca agtttgtctt tcatgtcatc atagaaggag
     5101 agatcgatat aaactgtgcc atccgccggg caatagaacg gccccataat ggactgacca
     5161 gccccgcagc cggtgcgcgt cattccacgg tacatgacca gcttcggttg ctgataggtc
     5221 ttacccatct tctcgaactg ttgtccccag gtgtcttccg tggttgccag aatcaccgag
     5281 gtgaattttg cagcttcgtc ttcattaggg ctaatggacc gcgttgattg ttgttgggaa
     5341 accggctgtc cagtcattaa cccggttaaa tcaacaccat agtagcctgc aaccagcacg
     5401 actaacagta aaatcaaccc gcctttaccg cttggcaggc gaaaaccggg accgcccata
     5461 gacggaccac cggagctgtt gcgcctgtct tcaacattgt cactttcacg tcgcccttgc
     5521 caacgcatag atacctcaac aatatattca ttatagaaat gatcgtaggt ggttcagagg
     5581 aagattacca caggaaagcg ggatgaaatg gcggaggaat taaggcttca tgccacgtta
     5641 tataatttac gaatgttaga agtaaaagag aatgttagct ccagtcttct agtctgattc
     5701 ccgccactcg ctcaaactct cgggtattgt tggtaaccac aatcaaaccg cgactgcgag
     5761 catgtcccgc tatcatctgg tcaaaaggtc caacaggcct tccctgccgg gcaagctcgg
     5821 ccctgatttg cccggtatgc gtcgcagcag cactatcata atcaagcact accaaacgag
     5881 atacgaaacc ttcaataacc gccaaattgc gttctggcat ctgacttttt tctgccccat
     5941 agataagctc catcaatgtt acagaactta tacacatccg gccagaattc aggttaaacc
     6001 gctctctgac
//
LOCUS       DAZFRJ010000233         5967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000233 DAZFRJ010000000
VERSION     DAZFRJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.235"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..1179
                     /gene="ompC"
                     /locus_tag="ACHKUN_004254"
     CDS             88..1179
                     /gene="ompC"
                     /locus_tag="ACHKUN_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6662392.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1291..2346
                     /gene="apbE"
                     /locus_tag="ACHKUN_004255"
     CDS             1291..2346
                     /gene="apbE"
                     /locus_tag="ACHKUN_004255"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO6662393.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2420..3484
                     /gene="ada"
                     /locus_tag="ACHKUN_004256"
     CDS             2420..3484
                     /gene="ada"
                     /locus_tag="ACHKUN_004256"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO6662394.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3484..4134
                     /gene="alkB"
                     /locus_tag="ACHKUN_004257"
     CDS             3484..4134
                     /gene="alkB"
                     /locus_tag="ACHKUN_004257"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO6662395.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4210..5853
                     /gene="yojI"
                     /locus_tag="ACHKUN_004258"
     CDS             4210..5853
                     /gene="yojI"
                     /locus_tag="ACHKUN_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO6662396.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GGASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1421 a         1515 c         1619 g         1412 t
ORIGIN      
        1 atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa aaaagcaaat
       61 aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct cctggtccca
      121 gctctgctgg tagcaggcgc agcaaacgct gctgaagttt acaacaaaga cggcaacaaa
      181 ttagatctgt acggtaaagt agacggcctg cactatttct ctgacaacaa agatgtagat
      241 ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa ctcaggttac tgaccagctg
      301 accggttacg gccagtggga atatcagatc cagggcaaca gcgctgaaaa cgaaaacaac
      361 tcctggaccc gtgtggcatt cgcaggtctg aaattccagg atgtaggttc tttcgactac
      421 ggtcgtaact acggcgttgt ttacgacgta acttcctgga ccgacgtact gccagaattc
      481 ggtggcgaca cttacggttc tgacaacttc atgcagcagc gtggtaacgg tttcgcgacc
      541 taccgtaaca ctgacttctt cggtctggtt gacggcctga actttgctgt tcagtaccag
      601 ggcaaaaacg gtagcgtaag cggcgaaggc atgactaaca acggtcgtga agcactgcgt
      661 cagaacggcg acggcgttgg cggatctatc acttatgatt acgaaggctt cggtatcggt
      721 gctgcagttt ccagctccaa acgtactgat gatcagaaca gcccgctgta catcggtaac
      781 ggcgaccgtg ctgaaaccta cactggtggt ctgaaatacg acgctaacaa catctacctg
      841 gctgctcagt acacccagac ctacaacgca actcgcgtag gttccctggg ttgggcgaac
      901 aaagcacaga acttcgaagc tgttgctcag taccagttcg actttggtct gcgtccgtcc
      961 ctggcttacc tgcagtctaa aggtaaaaac ctgggtcgtg gctacgacga cgaagatatc
     1021 ctgaaatatg ttgatgttgg tgcgacctac tacttcaaca aaaacatgtc cacctatgtt
     1081 gactacaaaa tcaacctgct ggacgacaac cagttcactc gtgacgctgg catcaacact
     1141 gataacatcg tagctctggg tctggtttac cagttctaat ctcgattgat atcgaacaaa
     1201 gggcctgcgg gccctttttt cattgttttc agcgtacaaa cacagttttt tggtgtactc
     1261 ttgcgaccgt tcgcatgagg ataatcacgt atggaaataa gctttacccg cgtggcactg
     1321 ctggctgccg cgctcttctt tgttggttgc gatcaaaaac cacaaaccgc caaaacccac
     1381 gctactgaag ttaccgttct tgaaggcaaa actatgggta ccttctggcg tgccagcatc
     1441 ccgggcattg acgccaaacg cagtgccgaa cttaaagaaa agattcagac ccagctggac
     1501 gccgacgatc agctgctttc gacctataaa aaagattccg cgctgatgcg ctttaacgat
     1561 tcgcaaagtt tatcgccgtg gccggtaagt gaagcgatgg ccgatatcgt caccacctcg
     1621 ctgcgcattg gcgcaaggac cgatggtgcg atggatataa ccgtcgggcc gctggtgaat
     1681 ctgtggggct ttggcccgga acaacagccg gttcaaattc cgagccagga acagatcgat
     1741 gcgatgaaag ccaaaaccgg cttacagcac ctgacggtca ttaatcagtc gcatcagcaa
     1801 tatctgcaaa aagacctacc ggatttatat gtcgatctct ccaccgtcgg tgaaggttat
     1861 gcggcggatc acctggcacg cttgatggag caggaaggga tttcccgcta tctggtgtcg
     1921 gtgggcggcg cgctgaacag ccgtggtatg aacggtgaag gcctgccgtg gcgggtagcg
     1981 attcaaaaac caaccgataa agaaaacgcg gttcaggctg tggtggatat caacggtcat
     2041 ggtattagca cctctggcag ttaccgtaac tattacgaac tggacggcaa acgtctttct
     2101 catgttatcg atccgcaaac cgggcgtccc atcgaacaca atctggtatc cgtgacggtg
     2161 attgctccga cggcgctgga agccgatgcc tgggatactg gcttgatggt actcgggccg
     2221 gagaaagcca aagaggttgt tcgccgggaa gggctggcgg tttatatgat caccaaagaa
     2281 ggcgatagct ttaaaacctg gatgtcacca cagtttaaaa gcttccttgt cagcgaaaaa
     2341 aattaaagcg caagattgct ggtttttgcg tgatggtgac cgggcagact aaaggctatc
     2401 cttaaccagg gagctgatta tgaaaaacgc cacatgctta actgacgatc aacgctggca
     2461 atctgtctta gcccgcgacc cgaatgccga cagcgaattc gttttcgccg tgcgcaccac
     2521 aggcatcttt tgccgtccgt cttgccgcgc cagacatgcc ttgcgggaaa atgtctcctt
     2581 ctacgcaaat gcctgcgagg cactcgccgc cggatttcgc ccctgcaaac gctgccaacc
     2641 tgataaagcc aatgcccagc aacatcggtt ggataaaatc acccacgcgt gtcgactgct
     2701 ggaacaggaa acgcctgtaa cgctggaagc cttagccgac caggtggcga tgagtccatt
     2761 tcatctacat cggttgttta aagcgactac cggaatgacg cctaaagcct ggcaacaggc
     2821 ctggcgcgct cgccgtttgc gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat
     2881 tcttaacgcc ggattccccg acagcagcag ttactatcgc aaagctgacg aaacgctggg
     2941 catgacggct aaacaattcc gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc
     3001 tgattgtgag ctgggtcgtt gcctggtggc agaaagcgag cgggggattt gcgcgatatt
     3061 gctgggcgat gatgacgcga cactaatcag cgagttgcag cagatgtttc ccgctgccga
     3121 caacgcgcct gccgatctga tgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa
     3181 tcaacgcgat acgccgctga cgttaccgct ggacattcgc ggcactgctt ttcagcaaca
     3241 agtctggcag gcactgcgca cgataccttg cggtgaaacc gtcagttatc agcaactggc
     3301 taacgccatc ggcaaaccga aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa
     3361 gctggctatc ataataccct gtcatcgggt ggtccgtggt gatggcacac tttccggtta
     3421 ccgctggggc gtgtcgcgta aagcgcaact gctgcgccgc gaagctgaaa atgaggagag
     3481 gtaatgttgg atctgtttgc cgatgctgaa ccgtggcaag agccactggc ggctggagcg
     3541 gtaattttgc accgttttgc ttttaacgct gcggagcaac tgatccgcga tattaatgac
     3601 gttgccagcc agtcgccgtt tcgccagatg gtcacccccg ggggatatac catgtcggtg
     3661 gcgatgacca actgtgggca tctgggctgg acgacccatc ggcaaggtta tctttattcg
     3721 cccattgatc cgcaaacaaa taaaccgtgg cccgccatgc cacaaagctt tcatgattta
     3781 tgtcaacgtg cggctacggc ggcgggctat ccggatttcc agccagatgc ttgtcttatc
     3841 aaccgctacg ctcctggcgc gaaactgtcg ctgcatcagg ataaagacga accggatctg
     3901 cgcgcgccga ttgtttctgt ttctcttggc ttacccgcga tttttcaatt tggtggcctg
     3961 aaacgtaatg atccgctcaa acgtttgttg ttggaacatg gcgatgtggt ggtatggggc
     4021 ggtgaatcgc ggctgtttta tcacggcatt caaccgctga aagcggggtt tcatccactc
     4081 accggcgact gccgctacaa cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa
     4141 gaattattat tgctgtgcgc gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg
     4201 gttttctgca tggaacttct tgtacttgtc tggcggcagt atcgctggcc atttatcagt
     4261 gtgatggcgc taagcctcgc cagtgcggca ttaggcattg gcttaattgc ttttatcaat
     4321 cagcgcctta tcgaaacggc ggataccagt ctgctggtat tgccggagtt tctgggatta
     4381 ttgctgctgt tgatggcagt cactctcgga tcgcaactgg cgctcaccac tttggggcat
     4441 cacttcgttt accgactgcg tagtgaattt atcaagcgga ttctggatac caacgtcgag
     4501 cgcattgaac aactcggtgg cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat
     4561 atcaccattg cttttgtgcg tcttccggaa ctggtgcagg ggatcattct cactatcggt
     4621 tccgcggcgt atctgtggat gctgtcgggc aaaatgttgc tggtaacggc tatctggatg
     4681 gcgatcacca tctggggtgg ttttgtactg gtggcgcggg tgtacaaaca tatggcgacc
     4741 ctgcgtgaaa ccgaagacag gctgtatacg gattttcaaa ctgtactcga agggcgcaaa
     4801 gagctgactc tgaaccggga acgcgccgag tatgtgttta acaacctcta cattcctgat
     4861 gcgcaagagt atcgccacca tattatccgc gcagacacct tccatcttag cgccgtgaac
     4921 tggtcaaaca tcatgatgtt gggcgcaatc ggcctggtgt tctggatggc gaacagtctc
     4981 ggttgggctg ataccaacgt tgccgcgacc tattcgttga cgcttttatt cctgcgtacg
     5041 ccgctgcttt cggcggttgg cgcattgccg acgctgctga cggcgcaggt ggcgtttaac
     5101 aagctgaaca aattcgcgct cgcgcctttc aaagcagagt ttccgcgccc gcaggcgttt
     5161 cccaactggc aaacgctgga gctgcgtaac gtgacgtttg cttatcagga taacgcgttt
     5221 tccgttggtc cgattaatct caccatcaaa cgtggcgagc tgctgtttct gattggcggc
     5281 aacggtagcg gaaaatcgac gctggcgatg ttgttgacgg gcttgtatca gccacaaagc
     5341 ggcgaaatct tgctggatgg caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa
     5401 ctgttttcgg cagtgtttac cgatgtctgg ctgtttgatc aactgctggg gccggagggt
     5461 aaacccgcta acccgcaact ggttgagaag tggctggcgc agctgaaaat ggctcataag
     5521 cttgagttaa gcaacgggcg tattgttaac ctgaagttat caaaagggca gaaaaaacgc
     5581 gtggcgctgt tgctggcgct ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg
     5641 gcggatcagg atccacactt ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag
     5701 gagatgggtc aaactatttt cgccatcagt catgatgatc attactttat ccacgccgac
     5761 cgcctgctgg aaatgcgcaa cgggcaactt agcgagctga cgggcgaaga gcgcgatgcc
     5821 gcttcgcgtg atgccgttgc ccggacggca taatattacg acgctgacct ggcggactgc
     5881 tgccgtcagg tcaatatgct aaaaatccct tcatttctaa taaccctata attaatcaac
     5941 gaaattataa tgtttctaaa attagaa
//
LOCUS       DAZFRJ010000234         5958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000234 DAZFRJ010000000
VERSION     DAZFRJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.236"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..329
                     /locus_tag="ACHKUN_004259"
     CDS             81..329
                     /locus_tag="ACHKUN_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6662397.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHKUN_004260"
     CDS             307..1437
                     /locus_tag="ACHKUN_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6662398.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKUN_004261"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKUN_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO6662399.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHKUN_004262"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHKUN_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKUN_004263"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKUN_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO6662400.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKUN_004264"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKUN_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO6662401.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKUN_004265"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKUN_004265"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO6662402.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKUN_004266"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKUN_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO6662403.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKUN_004267"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKUN_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO6662404.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5871..>5958
                     /locus_tag="ACHKUN_004268"
     CDS             5871..>5958
                     /locus_tag="ACHKUN_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662405.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFL"
BASE COUNT         1521 a         1400 c         1431 g         1606 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gaacatattc aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt
     5761 actgccgata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
     5821 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
     5881 gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg
     5941 cgctggccgt ctttttaa
//
LOCUS       DAZFRJ010000235         5906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000235 DAZFRJ010000000
VERSION     DAZFRJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.237"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            76..1227
                     /gene="yiaY"
                     /locus_tag="ACHKUN_004269"
     CDS             76..1227
                     /gene="yiaY"
                     /locus_tag="ACHKUN_004269"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO6662406.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1392..2930
                     /gene="aldB"
                     /locus_tag="ACHKUN_004270"
     CDS             1392..2930
                     /gene="aldB"
                     /locus_tag="ACHKUN_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO6662407.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3149..3352)
                     /locus_tag="ACHKUN_004271"
     CDS             complement(3149..3352)
                     /locus_tag="ACHKUN_004271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662408.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            3475..3798
                     /locus_tag="ACHKUN_004272"
     CDS             3475..3798
                     /locus_tag="ACHKUN_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6662409.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3804..4940
                     /locus_tag="ACHKUN_004273"
     CDS             3804..4940
                     /locus_tag="ACHKUN_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6662410.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4937..>5906)
                     /locus_tag="ACHKUN_004274"
     CDS             complement(4937..>5906)
                     /locus_tag="ACHKUN_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6662411.1"
                     /translation="KLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFES
                     KVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPT
                     GIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESL
                     VASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKE
                     VRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWWLD
                     LVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1457 a         1412 c         1543 g         1494 t
ORIGIN      
        1 ataataatga tcatgttttc gaatgaaaat cctcagtaag ctgcccaccc ttttttacac
       61 tttcaggagt gtgttatggc atcttcaact ttctttattc cttctgtgaa tgtcatcggc
      121 gctgattcat tgactgatgc aatgaatatg atggcagatt atggatttac ccgtacctta
      181 attgtcactg acagtatgtt aacgaaatta ggtatggcgg gtgatgtgca aaaagcactg
      241 gaagaacgca atatttttag cgttatttat gatggcaccc aacctaaccc caccacggaa
      301 aacgtcgccg caggtttgaa attacttaaa gaaaataatt gcgatagcgt gatctcctta
      361 ggcggtggtt ctccacacga ctgcgcaaaa ggtattgcgc tggtggcagc caatggcggc
      421 gatattcgcg attacgaagg cgttgaccgc tctgcaaaac cgcagctgcc gatgatcgcc
      481 atcaatacca cggcgggtac ggcctctgaa atgacccgtt tctgcatcat cactgacgaa
      541 gcgcgtcata tcaaaatggc gattgttgat aaacatgtca ctccgctgct ttctgtcaat
      601 gactcctctc tgatgattgg tatgccgaag tcactgaccg ccgcaacggg tatggatgcc
      661 ttaacgcacg ctatcgaagc gtatgtttct attgccgcca cgccgatcac tgacgcttgt
      721 gcactgaaag ccgtgaccat gattgccgaa aacctgccgt tagccgttga agatggcagt
      781 aatgcgaaag cgcgtgaagc aatggcttat gcccagttcc tcgccggtat ggcgttcaat
      841 aatgcttctc tgggttatgt tcatgcgatg gcgcaccagc tgggcggttt ctacaacctg
      901 ccacacggtg tatgtaacgc cgttttgctg ccgcatgttc aggtattcaa cagcaaagtc
      961 gccgccgcac gtctgcgtga ctgtgccgct gcaatgggcg tgaacgtgac aggtaaaaac
     1021 gatgcggaag gtgctgaagc ctgcattaac gccatccgtg aactggcgaa gaaagtggat
     1081 atcccggcag gcctacgcga cctgaacgtg aaagaagaag atttcgcggt tctggcgact
     1141 aatgccctga aagatgcctg tggttttact aacccgatcc aggcaactca cgaagaaatt
     1201 gtggcgattt atcgcgcagc gatgtaatca tcatttccac aacggctggc aaattgtcag
     1261 ccgctttttc aactatctct gtaacccttg cccgtaaatt cgtgatagct gtcgtaaagc
     1321 tgttaccgac tggcgaagat ttcgccagtc acgtctaccc ttgttatacc tcacaccgca
     1381 aggagacgat catgaccaat aatccccctt cagcacagat taagcccggc gagtatggtt
     1441 tccccctcaa gttaaaagcc cgctatgaca actttattgg cggcgaatgg gtagcccctg
     1501 ccgacggcga gtattaccag aatctgacgc cggtgaccgg gcagctgctg tgcgaagtgg
     1561 cgtcttcggg caaacgagac atcgatctgg cgctggatgc tgcgcacaaa gtgaaagata
     1621 aatgggcgca cacctcggtg caggatcgcg cggcgattct gtttaagatt gccgatcgaa
     1681 tggaacaaaa ccttgagctg ttagcgacgg ctgaaacctg ggataacggc aaacccattc
     1741 gcgaaaccag tgctgccgac gtgccgctgg cgattgacca ttttcgctat ttcgcctcgt
     1801 gtattcgggc gcaggaaggt gggatcagtg aagttgatag cgaaaccgtg gcctatcatt
     1861 tccatgaacc gttaggcgtg gtggggcaga ttatcccgtg gaacttcccg ctgctgatgg
     1921 cgagctggaa aatggctccc gcgctggcgg cgggcaactg tgtggtgctg aaacccgcac
     1981 gtcttacccc gctttctgta ctgctgctaa tggaaatcgt cggtgattta ctgccgccgg
     2041 gcgtggtgaa cgtggtcaac ggcgcaggtg gggaaattgg cgaatatctg gcgacctcga
     2101 aacgcatcgc caaagtggcg tttaccggct caacggaagt gggccaacaa attatgcaat
     2161 acgccacgca aaacattatt ccggtgacgc tggagctggg cggcaaatcg ccaaatatct
     2221 tctttgctga tgtgatggat gaagaagatg cctttttcga taaagcgctg gaaggctttg
     2281 cactgtttgc ctttaaccag ggtgaagttt gcacctgtcc gagtcgtgcc ttagtgcagg
     2341 agtcaatcta cgaacgcttt atggaacgcg ccatccgccg tgtcgaaagc attcgcagcg
     2401 gtaacccact cgacagcgtg acgcaaatgg gcgcgcaggt ttctcacggg caactggaaa
     2461 ccatcctcaa ctacattgat atcggtaaaa aagagggcgc agacgtgctc accggcgggc
     2521 ggcgcaagct gctggaaggt gaactgaaag acggctacta cctcgaaccg acgattctgt
     2581 ttggtcagaa caatatgcgg gtgttccagg aggagatttt tggcccggtg ctggcggtga
     2641 ccaccttcaa aacgatggaa gaagcgctgg agctggcgaa cgatacgcaa tatggcctgg
     2701 gcgcgggcgt ctggagccgc aacggtaatc tggcctataa gatggggcgc ggcatacagg
     2761 ctgggcgcgt gtggaccaac tgctatcacg cttacccggc acatgctgcg tttggtggct
     2821 acaaacaatc aggtatcggt cgcgaaaccc acaagatgat gctggagcat taccagcaaa
     2881 ccaagtgcct gctggtgagc tactcggata aaccgttggg gctgttctga tataagaagc
     2941 tggtcgcatt gggtattcat tgcctgatgc gacgcttacg cgtcttatca tgcctacggg
     3001 aacctgaccg taggccggat aaggcgtaca cgccgcatcc ggcataaaca aagcgcacgt
     3061 tgttacaatt tacgttttaa acctacgtgc gcaataaccc agactacgcc aacagaagtg
     3121 cgtttctggg gcgtgttttg ccaggagttt actctcgcgt ttcgatggtg agcgggcaaa
     3181 ccaggccgta aatcaccatt ggcgttccga ggccaatcag caataaacgg tagataattg
     3241 aaggtttaat acgctttatc tgtactgtgg atgttttact ggagttattc attagcttgg
     3301 tcaatagttc cattatttca atatgataaa cctcgacctg ttgaaaagcc acgcagcaca
     3361 cttcagaaag tatataaaaa acaatatgcc gttatataaa taaggaaatt gcactgcatc
     3421 atgggatttc ataatatgcg ttaattgttc ttttttatat tctctgaggg aaatatgttt
     3481 ctggattatt ttgcactggg agtgcttatt tttgtatttc tggtgatatt ctatgggatt
     3541 attattttac atgatattcc ctacctgatt gctaaaaaac gtaatcatcc tcatgccgac
     3601 gccattcatg ttgctggttg ggtgagtctt tttacgctgc atgttatctg gccatttttg
     3661 tggatttggg ccacgcttta ccgcccggag cggggatggg gaatgcaaaa ccatgactcc
     3721 tccgttgtgc aactacaaca gcgtattgcc gggctggaga aacagctcgc cgacatcaaa
     3781 tcctcttctg ccgagtaata cttatggatt tactgattat tttgacctat gtggcttttg
     3841 catgggcaat gtttaagatc ttcaaaattc ctgtaaataa atggaccatt cccacagcgg
     3901 ccctgggtgg catatttatt gtcagtggtt taattctgtt aatgaactat aaccatccgt
     3961 ataccttcaa agcgcaaaaa gcggttattt ctattcctgt tgtcccacag gtgacaggcg
     4021 tggtgatcga agtgacggat aagaaaaata cgctgattaa aaaaggtgag gtgctatttc
     4081 gactggaccc gacgcgttat caggcgcggg tggatcggct gatggcggat atcgttaccg
     4141 cagaacataa acagcgggcg ttgggcgcag agttagatga gatggcggcg aatactcagc
     4201 aggcaaaggc cacgcgggat aaattcgcta aagagtataa gcgttacgca cgtggcagcc
     4261 aggcgaaagt aaacccgttt tcagaacgcg atatcgatgt ggcgcggcaa aattatctgg
     4321 cgcaggaagc ctccgtgaag tcatcggcgg cggaacaaaa acagatccag agccagctgg
     4381 atagcctggt gttgggtgaa cattctcaaa tcgccagcct gaaagcacag ctcgcggaag
     4441 caaaatataa ccttgagcag acgatagtgc gtgcgccgag cgatggttat gtgacccagg
     4501 tactgattcg tccgggtacc tatgccgcgt cgctgccgct acgtccggtg atggtgttta
     4561 tacccgatca gaaacgacaa atcgtggcgc agttccgtca gaactccttg ctgcgactgg
     4621 ctcctggcga cgatgcggaa gtggtgttta atgctctgcc aggtaaggta ttcagcggta
     4681 agctggcagc cattagtcca gccgttcccg gcggagctta tcagtcgacc ggcaccttac
     4741 agacgttaaa cacagcgccg ggttcagatg gcgttatcgc gaccattgaa ctggatgagc
     4801 acactgattt gagcgcttta ccagacggta tttacgccca ggtggcggtt tactctgatc
     4861 atttcagcca tgtctcggtg atgcgcaaag tgctattacg catgaccagc tgggtgcatt
     4921 acctttatct cgatcattaa tcttcgcttt ccgccgcttg ttcaggccgc agttgctgcg
     4981 actgagcaag ctggtggcga atttcacgca gcatttcatc cagcacatac tggacccgcc
     5041 agggctggta ttcccgttta cggaagatag ccaccagatc cagccaccat tcatagggtt
     5101 gctgaaagca gggcaccagc gtgccgtctt gcaggtcgct ctgcacactt ttgtctggcg
     5161 caaacacaat ccccaggtga tttcgtgcca gctccagcgc agattgcgtg ttgtcgcaaa
     5221 cgtaatttcc cttcacgcga taatcccgca cctctttact cccggcgacg ttgaagcgcc
     5281 agatattcgc gtcgtcgatc atcatcgagt cgatcaaaat acaggagtga tgttcgagtt
     5341 cgtcaggacg gctaatggga tgtttctcaa gatagcgctg gctggcataa gcggttacgg
     5401 catattgggt aataaaactg gcaaccagcg attcatcttt tggtggagcg tagctgatta
     5461 aaacatcaca atcatcagga aattcgacgc cttcataaaa ggcattacgc tcaagattgc
     5521 aggtttttag cgataaggtg atatcaccga tatctttaat tttatcgatt acatgtttgg
     5581 ataaataggt aattatgccg gttggggcgt agatggtgac ccgaccacgt ttctcatgct
     5641 tataatctgc aataaaatta ttaagttgct cgtttctgtc cagcatgtcg ttgatgtacg
     5701 gtaacagcgc ggtgccaaaa ggtgtgagca tcagctgccg ggtggttcgg tcaaaaactt
     5761 ttaaacccac ttttgattca aaatcagcaa gatatttgct gacgttggcc tgtgcgatgc
     5821 caagtacagt cgcggcatgg ctgatatttt cactggcagc gattaccgag ataattttta
     5881 actcccggta cttaagttgt aatttt
//
LOCUS       DAZFRJ010000236         5867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000236 DAZFRJ010000000
VERSION     DAZFRJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.238"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(118..594)
                     /locus_tag="ACHKUN_004275"
     CDS             complement(118..594)
                     /locus_tag="ACHKUN_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO6662412.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(653..1942)
                     /gene="bioA"
                     /locus_tag="ACHKUN_004276"
     CDS             complement(653..1942)
                     /gene="bioA"
                     /locus_tag="ACHKUN_004276"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO6662413.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWATIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2029..3069
                     /gene="bioB"
                     /locus_tag="ACHKUN_004277"
     CDS             2029..3069
                     /gene="bioB"
                     /locus_tag="ACHKUN_004277"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO6662414.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3066..4220
                     /gene="bioF"
                     /locus_tag="ACHKUN_004278"
     CDS             3066..4220
                     /gene="bioF"
                     /locus_tag="ACHKUN_004278"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO6662415.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4207..4962
                     /gene="bioC"
                     /locus_tag="ACHKUN_004279"
     CDS             4207..4962
                     /gene="bioC"
                     /locus_tag="ACHKUN_004279"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO6662416.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            4955..5632
                     /gene="bioD"
                     /locus_tag="ACHKUN_004280"
     CDS             4955..5632
                     /gene="bioD"
                     /locus_tag="ACHKUN_004280"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6662417.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1377 a         1557 c         1657 g         1276 t
ORIGIN      
        1 gtttcgcgtt cagctttttt atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa
       61 gttgttttta atacctttaa gtgataccag atggcattgc gccatctggc agagtgatta
      121 actaaacatc gcagtaatcg aggcgcttgc cagagagtgg aaatgaacgt taaacccgac
      181 catcgcgccg ctggcacctt catcgacatc aatacgttct atatccagcg cgtgaacggt
      241 aaaaatgtag cgatgagttt cgcctttcgg cggtgctgcg ccatcgtacc cggttttacc
      301 aaagtcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag agccaaaccc
      361 ttgcggtaat acgcgggtat cagcgggtaa gttaacaact acccagtgcc accagccgga
      421 gccggttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac
      481 atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc cgttaaagac
      541 atgacgatgc ggcaatttat cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc
      601 tcctttcttg tttgcagaaa gtgtagccag aaaccctcac gcggacttct cgttattggc
      661 aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt
      721 gcgggagaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc cggatccaga
      781 caccctgttc gacaaagaat ttttgcagcg ccgccatatt caccggatga gtggtttcga
      841 ctacgccgat tgcccccagc acgcgcacat cggcaaccat ttcggcatca cgggcggggg
      901 caagttgctc gcgcagctgt acttcaatag ccgccacctg ttgctgccag tcgccagatt
      961 cgagaatcgc caggctggcg tttgctgccg cgcaggccag cggattgccc ataaaagttg
     1021 gcccatgcat aaagcaaccg gcttcgccgt tactgatggt ttccgcaacc tcgcgcgtgg
     1081 tgagcgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgagg cacaaaatgt
     1141 ccggcgcgat ttctgcatgt tcacaggcaa acagtttgcc ggtacgacca aatccggtgg
     1201 cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg attcgtttta
     1261 accattccgg atggtacatg cgcatcccgc ctgcgccctg gacaatcggt tcaatgatca
     1321 ccgccgcgat ttcatgacga tgcgccgcca tcaggcgggc aaagccaacc atatcacgtt
     1381 catcccattc accatccatg cggctttgcg gggcgggagc aaacaagttt tctggcagat
     1441 agcctttcca caggctgtgc attgagttgt ccggatcgca caccgacatc gcgccaaagg
     1501 tatcgccatg ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg cctttggcct
     1561 gccagtactg caacgccatt ttcatcgcca cttccaccgc tacggagccg gagtccgcga
     1621 gaaaaacgca ttccagcggt tgcggcgtca ttgccaccag tttgcggcat agctcgatgg
     1681 cgggcgcatg ggtgataccg ccaaacatca catgcgacat agcatcaatt tgcgacttca
     1741 tcgccgcatt aagctgcggg tgattatagc cgtggatcgt cgcccaccag gacgacatac
     1801 cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca accttcggcg ctcgcgaccg
     1861 gataaaccgg cagaggggag gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa
     1921 aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga tttaggttta
     1981 caattctaca ccgaattaac aacaaaaaac acgttttgga gaagccccat ggctcaccgc
     2041 ccacgctgga cattgtcgca agtcacagaa ttatttgaaa aaccgttgct ggatctgctg
     2101 tttgaagcgc agcaggtgca tcgacagcat ttcgatcctc gtcaggtgca ggtcagcact
     2161 ttgctgtcga ttaagaccgg agcttgtccg gaagattgca aatactgccc gcaaagctcg
     2221 cgctacaaaa ccgggctgga agccgagcgg ttgatggaag ttgaacaggt gctggagtcg
     2281 gcgcgcaaag cgaaagctgc gggatcgacg cgtttctgta tgggcgcggc gtggaagaat
     2341 ccccacgaac gcgatatgcc gtacctggaa caaatggtgc agggggtaaa agcgatgggg
     2401 ctggaggcgt gtatgacgct gggcacgttg agtgaatctc aggcgcagcg ccttgcgaat
     2461 gccgggctgg attactacaa ccacaacctc gacacctcgc cggagtttta cggcaatatc
     2521 atcaccaccc gcacttatca ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg
     2581 atcaaagtct gttctggcgg cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga
     2641 ttattgctgc aactggcaaa cctgccgacg ccgccggaaa gcgtaccaat caacatgctg
     2701 gtgaaggtga aaggcacgcc gctggcggat aacgatgatg tcgatgcctt tgattttatt
     2761 cgcaccattg cggtcgcgcg gatcatgatg ccgacctctt acgtgcgcct ttctgccgga
     2821 cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta tggcaggcgc aaactcgatt
     2881 ttctacggtt gcaaactgct gaccacgccg aatccggaag aagataaaga cctgcaactg
     2941 ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc tggcagggga taacgaacaa
     3001 cagcaacgtc ttgaacaggc gctgatgacc ccggacaccg acgaatatta caacgcggca
     3061 gcattatgag ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg
     3121 ccctgcgtcg ccgttatcca gtggcgcaag gagccggacg ctggctggtg gcggatgatc
     3181 gccagtatct gaacttttcc agtaacgatt atttaggttt aagccatcat ccgcaaatta
     3241 tccgtgcctg gcagcagggt gcggagcaat ttggcgtcgg tagcggcggt tccggtcacg
     3301 tcagcggtta tagcgttgcg catcaggcgc tggaagaaga actggccgag tggctgggct
     3361 attcgcgggc actgctattt atctctggtt ttgccgctaa tcaggcggtc attgccgcga
     3421 tgatggcaaa agaggaccgc attgtcgctg accggcttag ccatgcctca ttgctggagg
     3481 ctgccagttt aagcccgtcg cagcttcgcc gttttgttca taacgatgtc actcatctgg
     3541 cgcgactgct tgcttccccc tgtccggggc agcaactggt ggtgacagaa ggcgtgttca
     3601 gcatggacgg cgatattgcg ccactggcgg aaatccagca ggtaacgcaa cagcacaatg
     3661 gctggttgat ggtcgatgat gcccacggca cgggcgttat cggggagcag gggcgcggca
     3721 gctgctggct gcaaaaggta aaaccagaat tgctggtggt gacttttggc aaaggatttg
     3781 gcgtcagtgg ggctgcggtg ctttgctcca gtacggtggc ggattatctg ctgcaattcg
     3841 cccgccacct tatctacagc accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt
     3901 cactggcggt cattcgccgt gatgaggggg atgcacggcg cgaaaaactg gtgtcactca
     3961 ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac gctggctgat tcatgcagcg
     4021 ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt acaactggca gaaaaactgc
     4081 gccagcaagg ctgctgggtc acggcgattc gcccgccaac cgtacccgct ggtactgcgc
     4141 gactgcgctt aacgctaacc gcagcgcatg aaatgcagga tatcgaccgt ctgctggagg
     4201 tgctgcatgg caacggttaa taaacaagcc attgcagcgg catttggtcg ggcagccgca
     4261 ctctatgagc aacacgcaga tctacagcgc cagagtgctg acgtcttact ggcaatgctc
     4321 ccacagcgta aatacaccca cgtactggac gcgggttgtg gacctggctg gatgagccgc
     4381 cactggcggg aacgtcacgc gcaggtgacg gccttagatc tctcgccgcc aatgcttgtt
     4441 caggcacgcc agaaggatgc cgcagaccat tatctggcgg gagatatcga atccctgccg
     4501 ttagcgactg cgacgttcga tcttgcatgg agcaatctcg cagtgcagtg gtgcggtaat
     4561 ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt ggtggcgttt
     4621 accacgctgg tgcagggatc gttacccgaa ctgcatcagg cgtggcaggc ggtggacgag
     4681 cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg aacagtcgct gaacggcgtg
     4741 cattatcaac atcatattca gcccatcaca ctatggtttg atgatgcgct cagtgccatg
     4801 cgttcgctga aaggcatcgg tgctacgcat cttcatgaag ggcgcgaccc gcgaatatta
     4861 acgcgttcgc aattgcagcg attgcaactg gcctggccgc aacaacaggg gcgatatcct
     4921 ctgacgtatc atcttttttt gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg
     4981 aacggatacc gaagtgggga aaaccgtcgc cagttgtgca cttttacaag ccgcgaaggc
     5041 agtaggttat cggacggcag gttataaacc ggtcgcctct ggcagcgaaa agaccccgga
     5101 aggcttacgt aatagtgacg cgctggcgtt acagcacaac agcagcctgc agctggatta
     5161 cgcaacggta aatccttaca ccttcgcaga acccacctcg ccgcacatca tcagcgcgca
     5221 agagggcagg ccgatagaat cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca
     5281 ggctgactgg gtgttagtgg aaggtgctgg cggctggttt acgccgcttt ctgacacttt
     5341 cacttttgca gattgggtaa cacaggaaca actgccggtg atactggtag ttggtgtgaa
     5401 actcggctgt attaatcacg cgatgttgac tgcgcaggca atacaacacg ccggactgac
     5461 tctggcgggt tgggtggcga acgatgttac gcctccggga aaacgtcacg ctgaatatat
     5521 gaccacgctc acccgcatga ttcccgcgcc gctgctggga gagatcccct ggcttgcaga
     5581 aaatccagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt agctatcctg
     5641 tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta accaattaaa
     5701 ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg tgggaattgc
     5761 ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg ataaccatgt
     5821 gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttg
//
LOCUS       DAZFRJ010000237         5645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000237 DAZFRJ010000000
VERSION     DAZFRJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.239"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..520
                     /gene="fimA"
                     /locus_tag="ACHKUN_004281"
     CDS             <1..520
                     /gene="fimA"
                     /locus_tag="ACHKUN_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6662418.1"
                     /translation="ALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVNTDSADQVV
                     TLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAINDNLLAIASS
                     TNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGTGTSASAGQ
                     ANADATFIMRYE"
     gene            740..1432
                     /gene="fimC"
                     /locus_tag="ACHKUN_004282"
     CDS             740..1432
                     /gene="fimC"
                     /locus_tag="ACHKUN_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6662419.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1463..4072
                     /locus_tag="ACHKUN_004283"
     CDS             1463..4072
                     /locus_tag="ACHKUN_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO6662420.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4114..5091
                     /gene="fimH"
                     /locus_tag="ACHKUN_004284"
     CDS             4114..5091
                     /gene="fimH"
                     /locus_tag="ACHKUN_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6662421.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            5102..5617
                     /gene="sfmF"
                     /locus_tag="ACHKUN_004285"
     CDS             5102..5617
                     /gene="sfmF"
                     /locus_tag="ACHKUN_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO6662422.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
BASE COUNT         1478 a         1231 c         1356 g         1580 t
ORIGIN      
        1 tgcgctggct gccgcactat tcgccgctac gggtagttat gctgccgttg tagatggcgg
       61 tacaattcac tttgaaggcg aactggtgaa tgctgcctgt tcagtgaata ctgactcggc
      121 agaccaggtt gtcacactcg gtcaatatcg taccgatatt ttcaatgctg ttggtaatac
      181 ctctgcatta attccattca ccattcagtt gaacgactgc gatcctgttg ttgccgctaa
      241 tgctgccgtt gcattttctg gtcaggctga tgcaatcaat gataatttat tggccattgc
      301 atccagtacc aatacaacaa cagcaacggg tgtcggtatt gaaatacttg ataatacatc
      361 cgcaattctc aaacctgatg ggaatagctt ctcaaccaac cagaacttga tccccgggac
      421 caacgttctt catttttctg cacgttataa aggcaccggt acaagtgcat cagcagggca
      481 agcaaatgct gacgcgactt ttattatgag atacgaataa tcaaaaccac gttgttttga
      541 attatatatc acgtcttata acaaagtaat gtaccggttg tctgaagcgg tatggtggca
      601 atgtaaatcg aaatcatgtt cactttgtat catgccgctt tattaaatga aaagggaatg
      661 atgtgttgta agaaaccaaa gcaatcattt ctttatattt cttatttttg ccgtcaggaa
      721 tacacaaggc gtattaacta tgatgactaa aataaagtta ttgatgctca ttatatttta
      781 tttaatcatt tcggccagcg cccatgctgc cggagggatc gcattaggtg ccacgcgtat
      841 tatttatccc gctgatgcta aacagactgc ggtatggatt agaaatagcc ataccaatga
      901 gcgctttctg gtcaattcgt ggattgaaaa cagcagcggt gtaaaagaaa agtcattcat
      961 cattacaccg ccactgtttg ttagtgaacc caaaagcgaa aataccttgc gtattattta
     1021 caccggtcca ccgctggcag cagatcgtga gtctctgttc tggatgaatg ttaagacgat
     1081 cccttcggta gataaaaatg cattgaacgg caggaatgtt ttgcaactgg cgattttatc
     1141 gcgcatgaaa ttatttctcc gtctaattca attacaagaa ttacccgcag aagcaccgga
     1201 cacactcaag ttttcgcgat ccggtaacta tatcaatgtt cataatccct caccttttta
     1261 tgtcactctg gttaacttac aagtgggcag ccaaaagttg gggaatgcta tggctgcacc
     1321 cagagttaat tcacaaattc ccttaccctc aggagtgcag ggaaagctga aatttcagac
     1381 cgttaatgat tatggttcag taaccccggt cagagaagtg aacttaaact aaccgaatca
     1441 tctgacaata tcagagctaa ttatgaaaat acccactact acggatattc cgcagaggta
     1501 tacctggtgt ctggccggaa tttgttattc atctcttgcc attttaccct cctttttaag
     1561 ctatgcggaa agttatttca acccggcatt tttattagag aatggcacat ccgttgctga
     1621 tttatcgcgc tttgagagag gtaatcatca acctgcgggc gtgtatcggg tagatctctg
     1681 gcgtaatgat gagttcattg gttcacagga tatcgtattt gaatcgacaa cagaaaatac
     1741 aggtgataaa tcaggtgggt taatgccctg ttttaaccag gtactccttg aacgaattag
     1801 ccttaatagc agtgcatttc ccgagttagc ccagcagcaa aacaataaat gcatcaattt
     1861 actgaaagct gtacctgatg ccacaattaa ctttgatttt gcagcgatgc gcctgaacat
     1921 cactattcct cagatagcgt tgttgagtag cgctcacggt tacattccgc ctgaagagtg
     1981 ggatgaaggt attcctgctt tactcctgaa ttataatttc accggtaaca gaggtaatgg
     2041 taacgatagc tattttttta gtgagctcag cgggattaat attggcccgt ggcgtttacg
     2101 caacaatggt tcctggaact attttcgcgg aaatggatat cattcagaac agtggaataa
     2161 tattggcacc tgggtacagc gcgccattat tccgctgaaa agtgaactgg taatgggaga
     2221 cggcaataca ggaagtgata ttttcgatgg tgttggattt cgtggtatac ggctttactc
     2281 ttccgataat atgtatcctg atagccagca agggtttgcc ccaacagtac gtgggattgc
     2341 ccgtacggcg gcccagctaa cgattcggca aaatggtttt attatctatc aaagctatgt
     2401 ttcccccggc gcttttgaaa ttacagattt gcacccgaca tcttcaaatg gcgatctgga
     2461 cgtcaccatc gacgagcgcg atggtaatca gcagaattac acaattccgt attcaacagt
     2521 gccaatttta caacgcgaag ggcgtttcaa atttgacctg acggcgggcg attttcgtag
     2581 cggtaatagt cagcaatcat cgcctttctt ttttcagggt acggcactcg gcggtttacc
     2641 acaggaattt actgcctacg gcgggacgca attatctgcc aattacaccg cctttttatt
     2701 agggctgggg cgcaacctcg ggaactgggg cacagtgtcg ctggatgtga cccatgcgcg
     2761 cagtcagtta gccgacgaca gtcgtcatga gggggattcc attcgcttcc tctatgcgaa
     2821 atcgatgaac accttcggca ccaattttca gttaatgggt taccgctatt cgacacaagg
     2881 tttttatacc cttgatgatg ttgcgtatcg tcgaatggag gggtacgaat atgattacga
     2941 ttacgacggt gagcatcgcg atgaaccgat aatcgtgaat taccacaatt tacgctttag
     3001 ccgtaaagac cgtttgcagt taaatatttc acaatcactt aatgactttg gctcgcttta
     3061 tatctctggt acccatcaaa aatactggaa tacttcggat tcagatacct ggtatcaggt
     3121 ggggtatacc agcagctggg ttggcatcag ttattcactc tcattttcgt ggaatgaatc
     3181 tgtagggatc cccgataacg aacgtattgt cggacttaat gtttcagtgc ctttcaatgt
     3241 tctgaccaaa cgtcgctaca cccgggaaaa tgcgctcgac cgcgcttatg cctcctttaa
     3301 cgccaaccgt aacagcaacg ggcaaaatag ctggctggca ggtgtaggtg ggaccttact
     3361 ggaaggccac aacctgagtt atcacgtaag ccagggtgat acctcgaata atgggtatac
     3421 gggcagcgcc acggcaaact ggcaggccgc ttacggtacg ctgggggtcg ggtataacta
     3481 cgaccgcgat caacatgacg ttaactggca gctgtctggc ggtgtggtcg ggcatgaaaa
     3541 tggcataacg ctgagccagc ctttagggga taccaatgtt ttgattaaag cgcctggcgc
     3601 aggcggtgta cgcattgaaa atcaaactgg cattttaacc gactggcgcg gctatgcggt
     3661 gatgccgtat gccacggttt atcggtataa ccgtatcgcg cttgatacca atacgatggg
     3721 gaattccatc gatgttgaaa aaaatattag cagcgttgtg ccgacgcaag gcgcgttggt
     3781 tcgtgccaat tttgataccc gcataggcgt gcgggcgctc attaccgtta cccagggcag
     3841 aaaaccggtg ccgtttggat cactggtacg ggaaaacagt accggaataa ccagtatggt
     3901 gggtgatgac gggcaagttt atttaagtgg tgcgccattg tctggtgaat tactggttca
     3961 gtggggagac ggcgcgaact cacgctgcat tgcgcactat gtattgccga agcaaagctt
     4021 acagcaagcc gtcactgtta tttcggcagt ttgcacacat cctggctcat aaaggaaatt
     4081 atcaataaga taatctgcag attattattg gcgatggcat gtttgtgtct ggcaaacata
     4141 tcctgggcta ctgtttgtgc aaatagtact ggcgtagcag aagatgaaca ctatgatctc
     4201 tcaaatatct ttaatagcac caataaccag ccagggcaga ttgttgtttt accggaaaaa
     4261 tccggctggg taggtgtctc agcaatttgt ccacccggta cgctggtgaa ttatacatac
     4321 cgtagttatg tcaccaactt tattgttcag gaaactatcg ataattataa atatatgcaa
     4381 ttacatgatt atctattagg tgcgatgagt ctggttgata gtgtgatgga tattcagttc
     4441 cccccgcaaa attatattcg gatgggaaca gatcctaacg tttcgcaaaa ccttccattc
     4501 ggggtgatgg attctcgttt aatatttcgt ttaaaggtta ttcgtccctt tattaacatg
     4561 gtggagatcc ccagacaggt gatgtttacc gtgtatgtga catcaacgcc ttacgatccg
     4621 ttggttacac ctgtttatac cattagtttt ggtggccggg ttgaagtgcc gcaaaactgc
     4681 gaattaaatg ccgggcagat tgttgaattt gattttggtg atatcggcgc atcgttattt
     4741 agtgcggcag ggccggggaa tcgaccagct ggcgtcatgc cgcaaaccaa gagcattgcg
     4801 gtcaaatgta cgaatgttgc tgcgcaggct tatttaacaa tgcgtctgga agccagtgcc
     4861 gtttctggtc aggcgatggt gtcggacaat caggatttag gttttattgt cgccgatcag
     4921 aacgatacgc cgattacgcc taacgatctc aatagcgtta ttcctttccg tctggatgca
     4981 gctgcggcag ccaatgtcac acttcgcgcc tggcctatca gtattaccgg tcaaaaaccg
     5041 accgaggggc cgtttagcgc gctggggtat ttacgcgtcg attatcaatg aggtacggag
     5101 aatgagaaga gtactcttta gctgtttctg cgggttactg tggagttcca ttggatgggc
     5161 agctgaccct ttaggaacga ttaatatcaa tttgcacggt aacgttgttg atttctcctg
     5221 taccgtaaac acagcggata ttgataagac ggtagattta ggcagatggc ctacgacaca
     5281 attactgaac gctggcgata ccacggcact cgtccctttt agcctgcggc tggagggatg
     5341 tcctccgggt tcagttgcga ttttatttac gggaacgccg gcatccgata ccaacctgct
     5401 ggctctggat gatcccgcaa tggcacaaac cgtcgccatc gaattacgta atagcgatcg
     5461 ctcccggctc gcactggggg aggcgagccc gactgaggaa gtagatgcaa atggcaatgt
     5521 cacactaaac ttttttgcca attatcgagc gttagccagc ggtgttcggc ctggtgtggc
     5581 gaaagcggat gcgatattta tgatcaatta taattaatat tatattaatt cgtataattt
     5641 ggcgt
//
LOCUS       DAZFRJ010000238         5603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000238 DAZFRJ010000000
VERSION     DAZFRJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.240"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..296
                     /locus_tag="ACHKUN_004286"
     CDS             <1..296
                     /locus_tag="ACHKUN_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase NikB"
                     /protein_id="HFO6662423.1"
                     /translation="LIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNG
                     HFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM"
     gene            complement(517..2817)
                     /gene="trbC"
                     /locus_tag="ACHKUN_004287"
     CDS             complement(517..2817)
                     /gene="trbC"
                     /locus_tag="ACHKUN_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO6662424.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKVILNTLLPLEILLPVAE
                     NNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(2798..3922)
                     /locus_tag="ACHKUN_004288"
     CDS             complement(2798..3922)
                     /locus_tag="ACHKUN_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO6662425.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKY
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPKSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            complement(3919..5244)
                     /gene="trbA"
                     /locus_tag="ACHKUN_004289"
     CDS             complement(3919..5244)
                     /gene="trbA"
                     /locus_tag="ACHKUN_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO6662426.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFALLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     IGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEVFVAQNNLIRNMRLDVAATRQCFMAQLGQPLTTWKDMAPHEKALFAVFGLQ
                     FFLGDRKSAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEDAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            5547..>5603
                     /gene="dqlB"
                     /locus_tag="ACHKUN_004290"
     CDS             5547..>5603
                     /gene="dqlB"
                     /locus_tag="ACHKUN_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO6662427.1"
                     /translation="MINLLIIVLRAVVAVANAV"
BASE COUNT         1204 a         1374 c         1665 g         1360 t
ORIGIN      
        1 atttaattgc gccagttctg ttcagccgca acggaaagct tggttttgag ccggaaggtt
       61 cataccagca gtttaatgat gtgtttgcag aaatggtggc ctggcacaat gcagcgggaa
      121 taaccggaaa cggacacttc accattactc gtccggacgt ggatttgcac cgtcaacgca
      181 gtgagcagta ttaccgggaa tatatcagac agcagacccg tttatcggag agccatgatg
      241 ataattatac gttacggcag gagaaaacat gggagccacc atcacctgga atgtaagccc
      301 ggataagtga tggtgcaaag attcagccca aaaacggcag gaaccttcgt caataagggc
      361 gggagtagtc actgaattga ttttataccc ggttaactct gaattaaggg ccgtgtctgc
      421 ggcccttatc cagtgcgccg taaaaccccg gacttagtga acagggtttt acagtctgat
      481 tgacagatat ggtcaccaga aaatatttgt attccttcag tatttttcgt cagttgttgg
      541 ttgtgctcca catgatttct ggggatgatc attatttatt gtctgcgatt tattattttc
      601 agcgaccggt aacagtatct ccagggggag cagggtattc agaatcacct tcactgaagg
      661 tatatcttca ggaccggagt gttcctcttc cggacgattc agtgtgatat aacatacccg
      721 gcgttcttcc ctggttgtgt tattgatagt gtgcacagcc tttttccaca gttcgtttgc
      781 ccgggattcg atatctgtat catattgcgg aagcagatcc agtgatgtct gcgctgttgc
      841 caactggcgc tggaatgtat caatgatacg gtaaggctct gtacggtttt tacgacgttt
      901 acgggatggt tttgctgtca gtacaccgat gatgctgctg acatgctccg gtgtggggag
      961 cctgcgctgc actgtcctgg ggacgagacg gcggagacgc tccagccggg gggcttcaac
     1021 ggaaatatag cggttgatga ccaccggatc actgtcgcaa cttttctcgc tgtccggaat
     1081 atacacagag gctgacggca ccacatcccc ctggaaaacg gtaaagaact caccggcatt
     1141 cagtctgatc aaatcctgca cgtcgatttt gctttccatc tggatggtga tctggttacc
     1201 gtccacccag gactcaccca tcacaccatc gtggcgctgc aggctgccca ttctggcccg
     1261 tgcctcttca ccggcggcat ttttcaccgt tcttgccgtt ttgtcgtcac tgacaatgcg
     1321 tccggcgaat ttggtgccgg cgttctgcat cagtgtggcg gtgttggtgg cggaggtctg
     1381 cccttcaatt cgctcctgat cctgcgccgt cattatcagg gaaaactcca gtgaacgtac
     1441 ctgggtggcc tccacagcaa tgcggtctgt gtagtaggca ccgacttcgt caaggataca
     1501 gatatagggg aagctgcctt tgtatttttt gacttccagg gtctgtgcat ctgtgccttc
     1561 cagccggtag cccaggtcac gggcgaggag catgctctgc tgggtgacga acatacgacc
     1621 gagtgccgac gtggtatgag ccgatgtgtc cagtgcaggg atcatcacaa tcagaatgcg
     1681 gtcgctgtga atactgtcgc gaatatcgat gtcgccggca tctgccgcaa agacgtcccc
     1741 gaaggtttcg gcaaaggttg tgaacgtttc cgagaactga ccggagagat aagcatgctg
     1801 tttacggggc tcctcagtcc aggctgacgg cgttctcacc agtgacatat cgaatcccgg
     1861 gacatcctgc aggtagtttc gcagtggtgc gatagcttct tccggccact ggtcatcaag
     1921 gccacggcaa tagagttttg ccatcccttc cagcgtcata tgttcccgta acatctgcag
     1981 ggacatggtt ttaccttccc ggacgcacca gaattttgtg ccaaagacca gggccttgtt
     2041 catggctatc gcccgtgact gccattcacc gccctgaacg ttctggggca gcatggactg
     2101 catggtttcg gcggtgaagg cttcggtgct gaatgcgaac gggttccagg tgtttgactg
     2161 tgggcgggat ttttcgccgc tctgaatgat ttcactgcgg gattttccgc cgttcatgaa
     2221 gttgatgacc tcgatatcat cctcccggcc aaaacgtcgg gagagatacc agattgtcct
     2281 cgtggtatcg ttctgtgcct ttccgtcaac aaaggtaaag cctcttcccc agcagagcgg
     2341 gttgagcagc caggcaaagg tggtttctgt tttaccgcca ccggtggtgg caaaaaacat
     2401 gacatgccgg gtgaggtcat ccatgctcag ccagagctca cgaccgatat cattgatacg
     2461 gcggtaaccg acataaaaaa tcccccgtgc gggggatgtt tcatctgctt catactgaaa
     2521 cagggtgggc cagaagctga agaggcttcg cctgaccttt ctgtcctggg aggggtcatc
     2581 aaggctgagc gtcatgggca tacgcagggg gcatcgccag cgttgcagcg tgaatatcac
     2641 ggaggtgacc agggtgaaca gcagacagac aggaagggcc agtggccata tcagggccgt
     2701 aatgatactg ccggccagac acagaccaat gatgttcagg ttatgcagtg cattccacag
     2761 cgggtttccc caggccgtgc ggtgtatgag gtcaggatta acgtggtgct ggctcattgt
     2821 gatactcctc gctatctgtt gctttcagga agaggcgaac ggaagtggcg tctttcagga
     2881 tgccggacgg caggtgccag ccggtatctg ataacaccca gggggttccg ccaaaaccga
     2941 actggcggaa agcctggtta ttgacatcga tggcagcacg tgcggcggtc agacactgtc
     3001 catcctgtgc ggccggtgca tcgccggtgg gggccatacc actgtcagcg gaatacagct
     3061 gatgccatcc ggtcgcccgt ttttgtgagt ccggttcaca cagcagcgga gcagtccgct
     3121 ttgtactgga ctccccgcca atcacggaga ccggataaat caccacgctg aattcccggg
     3181 ccagttgctt aagcgcgggt tccagcagac ggcagttgat gcatgcgggg tcaaggaagg
     3241 cgtacagcgt gcgttcatga ccagtggaca ggttaatggt gaacatcccc cggtctgcgt
     3301 tgcgtttaag tactgtggcc agattacggc gggcctcagc ggcctgaacg tgttgttctt
     3361 ccgtcactgc cgtcggggcg ggggcagaag aaggttcctg ctgtgtggga gccgggcggg
     3421 ccgggatgct gttaacatga tgttccgtat gtgggcgtgg tgtctctgtg tcggttactg
     3481 gttcagctct ccattcctga gatttagggt aagccagcgt ggaagggggc tctgccttca
     3541 tccagtggta acctgcaact gtcgccggca gagcaattcc ggcgataaag ccacacatca
     3601 gtgtccgcca gaagccatat ttccgggagg gatgttttcc ttttccggca tattcggcat
     3661 ccaggcaggc atcaatcgct gagagcattc tgccgatgtg atgtccggga cgttctgcat
     3721 caacggtatc aacaagcacc gtttcggtta tttcgcgaaa aggggaactg aaggacaggt
     3781 gcatcagcct gttattacgg ttaatcagtg cgcccagttc tgttaccggc aggatggcaa
     3841 tcacctcccg attaccgtca gggtacaggg cggtcaggga caggtgttta ttattcagtg
     3901 ttgcagtgta gtggatggtc attgttcttt ttccttgtcg gcgggatgct gtttttcggt
     3961 ggtttcggtg gtttcggtgg tttcggtggt ttcggtggtt tcggtggttt cggtggtttc
     4021 ggtggtttca ccagtgtcag tgtcttcgtc gttatcatgg cggcggggta tcacgtcatc
     4081 cgtgagtgac cagcgggtcc tgatctgacg tttacgatcg cgatctggct gaggttcgtc
     4141 ccatatcagg cccagcccca gcatgtcccg tcgaagccct tcaatggcct cttccacaca
     4201 gggttccggg caggggaggc cgagacggct ggcttcattt tctgcccggg caacggccac
     4261 cacgccggcc ccttcgatga agccttttgt ggtgtttgcc cggtgaaggg catagaagag
     4321 cgtccggtca acgcccttga gccacagcca gttggggggc gtcaggcgca aatcatgtgc
     4381 gtaaagccag acaagaccgc tgcgaacgta acggtgctgc ctgagccact gttgtgcgtc
     4441 ttctgttttt atgacccgga taaaggcgtt tctggccagt gaatacactg gcgtgctgaa
     4501 acgcccctgg tcccgcttgc ttttgagtct gcaggagagg ttgaggttat tcatcaggga
     4561 aacagccgat ttccggtccc caaggaagaa ctgcaggcca aagacggcaa agagtgcttt
     4621 ttcatgtgga gccatgtctt tccaggtggt cagaggctgg cccagttgcg ccataaagca
     4681 ctggcgcgtg gccgcaacat cgagccgcat gttgcggata aggttgttct gtgccacaaa
     4741 gacttccggg gttagagcga cacggcgctc gggacgtttt tgtccgtgga agagtctgtt
     4801 gctgtctccg tcagccagaa cgggggcgat agccggtgac agtgatgaaa acagatgtgg
     4861 cagcgtgtgg atattcagtg gtctgcgggt gaagcggggc tgggccggat gctgaaacca
     4921 catccacaga aagccgatcg tcatgggaag cagaatcagc cagaggatcc ccacggtctg
     4981 tgacatgaca tccagccact ggctgaaagt gatcgttgca gcgttgttgg cggtgttggc
     5041 cagcaggtta taacgggggg cggcaaaatg gtgaaaaggc ccccagtcag tcatccccca
     5101 cattgtgtgc aggatcaggc aggtggcaaa gacgatgtcg ggtaaaaaga gccagatgag
     5161 aagcgcaaag agcagacata tggtgccaac gatataccat atgttcatcg ggtcttctgt
     5221 tgtgggggag tgttggtaag acacgaggat tcctccatcg ataaatagat aaatttcggg
     5281 atgaggctga gtggaattaa tgcgaacaat cgccggtaat gaccgggcag ggctgaattc
     5341 agcagggcat tttcacagga acagcagaag gtggtgagag acaggcacaa aaaagccgac
     5401 tggtgaggcc ggcttttttg cgatcccagg gagagtgggt ggctctggcc cggaggaagt
     5461 ctcttttcag ctaacaatga aagagctgtt gtgacaggca atgtaagagt aaacaacagt
     5521 gatttcctcc attgatggag aaacggatga ttaacctgtt aatcatcgtt ctcagggcgg
     5581 ttgttgctgt ggctaacgca gtg
//
LOCUS       DAZFRJ010000239         5573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000239 DAZFRJ010000000
VERSION     DAZFRJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.241"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(274..882)
                     /locus_tag="ACHKUN_004291"
     CDS             complement(274..882)
                     /locus_tag="ACHKUN_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO6662428.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1308..1376)
                     /locus_tag="ACHKUN_004292"
                     /pseudo
     CDS             complement(1308..1376)
                     /locus_tag="ACHKUN_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1635..1982
                     /locus_tag="ACHKUN_004293"
     CDS             1635..1982
                     /locus_tag="ACHKUN_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662429.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2187..2888)
                     /locus_tag="ACHKUN_004294"
     CDS             complement(2187..2888)
                     /locus_tag="ACHKUN_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6662430.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2885..3904)
                     /locus_tag="ACHKUN_004295"
     CDS             complement(2885..3904)
                     /locus_tag="ACHKUN_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6662431.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3901..4440)
                     /locus_tag="ACHKUN_004296"
     CDS             complement(3901..4440)
                     /locus_tag="ACHKUN_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO6662432.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(4510..4740)
                     /locus_tag="ACHKUN_004297"
     CDS             complement(4510..4740)
                     /locus_tag="ACHKUN_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6662433.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4845..5534
                     /locus_tag="ACHKUN_004298"
     CDS             4845..5534
                     /locus_tag="ACHKUN_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6662434.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
BASE COUNT         1416 a         1320 c         1268 g         1569 t
ORIGIN      
        1 ttttatgatt atacccttat caggtatgat gtaggaactg aaaacaatat tggatatcat
       61 tttcggtatt tctctgcaaa ttccagccca caggatctcg gtgtacaatc tgtcgctgat
      121 cgcgcaaatt tagggtcaat cacttcattt tttgcatcaa accctcacta acatcgaacg
      181 attatagtac aagtagtgtt cttgacttct cattaaaccg tcagcattca attaattaca
      241 aaatactagc ggcatttctc tcccgcaact ttattacagc cgttcagaac gtaaatgcgt
      301 caatactcga ctccacttat catcctgcca cggccggaat tttacatgtg ccgtttctct
      361 ggcaaggatt gcctgtgcct tattgagtat ccggggatat tcttgttcga ttgaagtgaa
      421 gcgaccggct tcgcggtgtt ctgctacctg aagaagcgga gtgacgtttt ggcaagcggt
      481 taacattgtg tcacttgccc gccataacca agcaagtgtg caaagttcgt tatcagtgaa
      541 ttgttttgtg attggggatt gttgaatttc tcgatcgaga atatccagca cccagcggcg
      601 gaattctttg gccacaggag tgcgggcgaa catggcgatc agatgggcgc cacggaggga
      661 gaaaatgcga gtcttcttac gataatttcc tgaggtcgtt gattcgacga cctgagacat
      721 tccgtcagta aactcatcaa tatttttgtt gtagatgtcg gttactgctt tggtgctggc
      781 atattttaat gctgccgcca attctttgct ggtaaaccag atctggttgt tgtgatttat
      841 aggagtgaat tttacatcgc ggaatgcgag ctgagtgacc atagcgataa tctcgttttg
      901 aatttgagct atcaccaccg acgacgccaa tcgactggtg gtggactgtg caaggttggc
      961 gtaaccggtc aaaacgaaat ccggcgcttc cgaagaagcc ctcacacagc ccaccataat
     1021 ttttgtgtag acgtgcactg cgcataaaaa aaccgcgaac gcggttatgc gtcgttttga
     1081 tgtccgggac gccaatcccg tgtgccgatt ttgcggcaac gcacagaata tagcgccgga
     1141 taaataatgt cgtcaaccct tgcatatggg gggcgcgatg atcactacta ccaccataat
     1201 cgctccaact gttgcaacta ctttaggcta agacatcacg atttccatat cacctttagc
     1261 cgccagacaa acagagactc cctgttcttg gctgccaaaa acaccgaatg caaaaaatcg
     1321 caggtgtatt tctgcatctg tggagggaga agagagtttg gattgtatgt gcgcataaac
     1381 gtccccgttt agcgcagaag tcaccgtagt tgttcatatt ctgatgacat gataatttcc
     1441 tattgaatag aaaaaattaa cacgactcat tcttatgact ggaaaaagca atcatgctaa
     1501 taatcattgt tgttccagtt ccaaatgcag cgaaataatc accaatattt tttctaccgg
     1561 tttcgacttt gatgctatat tctgctaaca cctaactaac actacgtttc acacagcaat
     1621 tacatggaaa taacatgact atcaaagcga ctgacataga gattcttcat cgttatgctg
     1681 aaggggtaat ggaacgttct aaccatcatg caaaaaatgt tggtgcagcc gctcttacat
     1741 tgctaggagg tgttatctgg aaagccctgc ccggatcaat cgagataaga acgtataacg
     1801 gaagtcttgc aaatatggtg tggtggcaaa gcgaaagaac attaaaaaat tatgcaatct
     1861 catacaacca taattcatgt gaaattgaaa tgagagatga atcagtcaaa ggtgctgtat
     1921 tatttagcat ttccaacgaa accactccag aaaaaatatt atcacagctt tctgagcttt
     1981 aattaaaggc ggtgctaaca ccgcccatac cattactcca catcttacgc aacttgataa
     2041 ttatgctcta ttaaataccc acaatatttc atcaatgcat gggtcagcta caaaatgttt
     2101 accgtggtaa ttcctgtttt cgcaacatct caaaagaaca ataaacactg tatttcttca
     2161 agtaacgaag aatctttctt tgcccgtcat acacttgctc ctttcagccc aaacttagct
     2221 ttgatttctg cgatcttcgc cagagcctgt gcacgattta gaggtctacc gcccataaca
     2281 ggaagttgtt ttactggttc aggtatcgtc tcaccacggt taattcgcgc tgtcatacag
     2341 gtcagttcat cggcagcctt gcgccgtaat tccgcgtcag tcagcgcatt ggcccgcatg
     2401 ttctggtaca ggttggtaac caaccagtaa tgcgcgttcg atttccacgg ataagactct
     2461 gcatccggat acaggccacg cttccggcaa tactcgtaaa ccatatcaac cagctcgctg
     2521 acgtttggca gcccggcgtt aacggatgct tcttcccggc accatgcaac aaactgcccg
     2581 ggtgatggca gaaatggtct attctgccga cgggcaacac gcattccggc gctcacctgt
     2641 tccatcgtgg tgatcccgtt ttcccggaaa gccagaaccc actggcggcg gatttcgttc
     2701 agttcgttct ggtcacgatt agccaggctc gccgggaaag ttgccagcaa ctggctgaat
     2761 acaccgttga ttatctgcgc tacctgctgt acctgcggct tttcgtcgta ctgttccggc
     2821 atgttgttgg cgatccgacg catctgctca cggtcaaagt taatcatctg tgcggcgatg
     2881 tttttcatag atccaccccg taaatccagt ctgtgtttgt caggtcgagt tttggtttgc
     2941 cgactgtcac gccagcctgt tgcttgttcc ggttgatttc gagctgggtc cacttgtcgc
     3001 ggagtttggc cggactcagc acgttaccgg accagaagtt gtcctggcag gcccagcgga
     3061 acagtacaca catgtcgcgg tggttacgtc catcacgttc acgcatcaga cggatatcgt
     3121 tagcccaccc tgcaaaattc ggttttctgg ctgatggcgc gatggtcttc accatgtcaa
     3181 acatccactc tgcggcggtc aggtcttctg ctgtccccca cttgctgccg ctctgaatcg
     3241 cagcatccgc tttcaccaca ggaaggtcgt tttctggcag gtcagaggat tcgccagaat
     3301 tctcggacga ataaggtttt atattgtctt ttgttagttt gtcttttgtg tttacctgat
     3361 tcgggtaagt gcctttacct gatttgggta aacttttctt acctgattca ggtaaattta
     3421 cctctttcag gtaaacttta tttttcttac ctgattcggg taatgttgac cattcactga
     3481 ccacattatt aatgccgata ttccgcccgc tctgaataaa aatcccacgc tttaccagaa
     3541 cactttttgc agcagaacac ttgtgcggca atatcccggt caactcggaa agttgctcgt
     3601 tgctcaccca gtccagtttt ttattaaagc catatgtttt gcgcatgaca gccaggaaga
     3661 ccagaagctg gtgctgtgtt aatccggcca gcattacagc ttccagcaac tcatttgcaa
     3721 tgcgcgtata accatcatcg agatctgcca cgcgcggctc cttttgtgcc gcatccggca
     3781 ctggaaaatt gaatatctca gcagtgtttg ccataattcc tcccgcaatg agtgtgttac
     3841 gatttgcacc tgaaagtcgg ttctgttcca gcagaccggc tttcgccatt tctgaacctg
     3901 tcatatcgcc cccagcatgg tagtaaccat cgccatcaat ggaccagcca gctctgggtc
     3961 cacacgaaac atcgacacaa taccttcact aatttccttc agtttctggt ggcgtggtgc
     4021 gttgagaatg acagcctgtt ttgcctcact gagttccttt tccatttcag ccaacctagc
     4081 catgaagcta tcctgctcaa ccaggtaacc gcgatattcc agcggtagta ccgccagaat
     4141 tgccggggtc agttcacgca cgttatttcg gtatttttca gaatcgaatt tgttatcgag
     4201 gaagcggaac agcttctggc gtgtacggct gacatcatca gggaaatcga tggtgccgcc
     4261 gccctgctcc cgatactcat tcacaatgag tgtggcaacg acatcctgat tatctacagc
     4321 cgaccaggcg cggacggcat cacggatttt ttcgtggcct ggcacctgtt ttgtttgaga
     4381 acgatttatc accgcagtcg ggctaaatcc gctagtctgt tggtatggaa gtggttgcat
     4441 aattgactcc tttagtttga attgactgtt aagttgattg cttattgtta aagagcgtga
     4501 aatggaaatt taagctgcgt tcttttcggt gtgtggaaac aacttcggaa gatccgggcg
     4561 aatctggtat gccttcacta ctccaccagt agccgtaaca atgctgccga catgttcagg
     4621 ggataccttt gctttgttgt gaagccactt atagacggcc tgctgtgaaa cttcgcaggc
     4681 agcgcccagt ttcttttgtg aaccaacgat attgatcgct gttttgatag ctgggttcat
     4741 aacaacctcc gtggttaatt tgaatcaaga ttaaaactat ggttgttttt agtcaacaac
     4801 cattttcgtt tgatggaata aaaccttggt tgtacatttg gactatgaaa acaacactct
     4861 cagaaagact taaagaagcc agattagcgc gaggccttac acaaaaggcg cttggggatt
     4921 tggtcggggt tagccaggct gctattcaga aaatcgaaac agggaaagct aatcaaacaa
     4981 ctaaaatcgt ggagatcgcg aacgctttgg gtgtgcgcgc agaatggtta tcttctggcg
     5041 ttggaaatat gtcagacagt acagtgcaac caatacaatc aactgtcagc cattccaaat
     5101 acttcaagat tgacgttctt gatatagaag tcagtgctgg gccgggagtc atcaaccgtg
     5161 agtttgtaga agttctacgc tcggttgagt actcgtttga cgatgctcgt cacatgttcg
     5221 atggtaggaa ggcagaaaat atccgcatca ttaacgtgcg tggtgacagc atgtcaggaa
     5281 cgatcgaacc aggtgatctg ctgttcgttg atatcacggt taaatctttc gacggtgatg
     5341 gtatctatgc atttctgtac gacgacaccg cccatgtaaa gcgcctgcaa atgatgaagg
     5401 ataaactgct ggttatctct gataacaaga gctactcacc gtgggaccct atcgagaaag
     5461 acgagatgaa ccgggtattc atattcggta aggttattgg gagcatgccg cagacgtaca
     5521 gaaaacacgg ataatcagcc gcgtgttgat gaggcttttg ggtaatacgc tga
//
LOCUS       DAZFRJ010000240         5558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000240 DAZFRJ010000000
VERSION     DAZFRJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.242"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            126..1484
                     /gene="mltD"
                     /locus_tag="ACHKUN_004299"
     CDS             126..1484
                     /gene="mltD"
                     /locus_tag="ACHKUN_004299"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO6662435.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1532..2302)
                     /locus_tag="ACHKUN_004300"
     CDS             complement(1532..2302)
                     /locus_tag="ACHKUN_004300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6662436.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2380..3180)
                     /locus_tag="ACHKUN_004301"
     CDS             complement(2380..3180)
                     /locus_tag="ACHKUN_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6662437.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3421..4335
                     /gene="yafC"
                     /locus_tag="ACHKUN_004302"
     CDS             3421..4335
                     /gene="yafC"
                     /locus_tag="ACHKUN_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO6662438.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHKUN_004303"
     CDS             complement(4332..5135)
                     /gene="dkgB"
                     /locus_tag="ACHKUN_004303"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO6662439.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5298..5374)
                     /locus_tag="ACHKUN_004304"
     tRNA            complement(5298..5374)
                     /locus_tag="ACHKUN_004304"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5338..5340),aa:Asp,seq:gtc)
BASE COUNT         1451 a         1344 c         1372 g         1391 t
ORIGIN      
        1 caacaacctg aagagcgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac
       61 tttcttgtca tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca
      121 cacacatgaa ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagta
      181 ccggcaacgt tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa
      241 agtttacaag tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact
      301 tgtgggcttt cattggcgac gagctaaaga tgggaattcc ggaaaatgac cggattcgcg
      361 aacagaaaca gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag
      421 agccgtatat gtactggata gcagggcaag ttaaaaaacg taacatgcct atggaactgg
      481 tactactacc catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg
      541 caggcatctg gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacccgca
      601 attatgacgc gcgtcgcgat gttgttgctt caacaactgc cgcgctgaat atgatgcagc
      661 gtctgaacaa gatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg
      721 aaggtcgagt catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct
      781 ggtcgttacc gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg
      841 atattctcaa aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg
      901 ctctggcgcg tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca gatatggcgg
      961 ggatttccgt cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg
     1021 caagtggtcc gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc
     1081 tggcttcagg cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta
     1141 acagccgtgt ttacaccgta cgctctggcg acacgctttc aagtatcgct tcacgtctcg
     1201 gcgtaagcac caaagatttg cagcagtgga acaaactgcg cggatctaaa ctgaagccag
     1261 gccaaagttt gacgattggc gcaggcagta gcgcacagcg actggcaaac aacagcgata
     1321 gcattacgta tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg
     1381 tgaacatcaa agatgtgatg cgctggaaca gcgacactgc gaatctgcaa ccaggcgata
     1441 agctgacgtt gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataataaa
     1501 aaggcaccga ttcccccggt gcctttttta tttatgccgc tttatgtgct tctaccatga
     1561 tgatatcact ggtgaaagag ccgtcattct gcaaggcaaa atacgttctc acctctgtcg
     1621 atgcgctctg ttggtaaatg cgaatagcgt ctattaacgc ttctggcgta cgcattctca
     1681 cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca actatcagat
     1741 tggcttcatt gattaacgtc aaccactcac cgctggcgta gtttcgtacg tgagaggtat
     1801 cgcgtaatgc ttctaccgtc tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta
     1861 cgtccatcac aatcagccta ccgccaggtt tcaatatccg gttcacttca cgcagtgctg
     1921 caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca aatgcgttgt
     1981 cggcaaatgg cagactttcg gcataaccct ggcgggtggt gatatttttc agttgccgga
     2041 cttcggcagc ttgtgccacg acatctagca tgtgggcaga taagtcatac gccaccaccg
     2101 tgctcacgtt ttgcgcagcg acaaagctgg catgccctgc tccgcagccc atatcaagta
     2161 cacttgcacc aggataatca gccagacgca ccgccaggcg ctgtaaatct cgtccggatg
     2221 catgtacggt actggtcaga tattcgctgg cttgagagct aaactgcttt tctacatggt
     2281 catggtggga ttgtattgtc attgcgctgt ccttttgtta tttgagtata aaagggcagc
     2341 gccaaacgtt ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg
     2401 actgaattca acgagtagcg gattgtgatc ggaagcgcgc gtaaccagta cagaagcttc
     2461 gctgacgttc agaccacggt agaaaacaaa atcgagcggg cgaccaaacg cccgacggcg
     2521 ctgatcatcg gtaaaacgca cctggcgcag cgacatttcc cgcgcaaagc gatataacgc
     2581 gttcatcctt ctacggctcc aggcattgaa atctcccgcc ataatgaccg ggccgctgtg
     2641 gtgagctatc tgatcaccaa taggaagtaa ctgcttacta tagacatcca cgcccagact
     2701 gaagttgacg gcgtgtatat taaccaccat caacaggcgg gtgtcaggta atggatagac
     2761 cgtcaccagt gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca
     2821 atacactgga tgcgccgccg aaagggtcat tacaccggaa ggatgttgtg gcagcacgaa
     2881 agcgggcacc tgatcggcgg caagatagtt agcggtcgca aactgtacta actctggcgt
     2941 tgtctgcgct tcctgcaata acaccagatg tgcatctttg ccgtagttct ttaataccga
     3001 caaccattca gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc
     3061 agtacttaac ggttctccag gtggtaatgc ctggccgatg ctcgcaaaag accccggcgg
     3121 taagatcctt tccgcaggtt gtccggcaac atagcgcatg gcataggtgt tttttcgcac
     3181 ttttgacttt gaaacctcta ttacgtcaac ccttccgcac tcgggagggt tctactgtta
     3241 tagggatttt agctcactct ttcaacgagc aattgtatat tgttatgtaa gcaagtgctt
     3301 acacaggtca gaattttgct cattattgag aacattaaat cggatgacat atcacgcatc
     3361 ctgatggttt aatgacaaat aaattcaaaa tatctcttct attttgcaac aggagcaaaa
     3421 atgaaagcca cgtcggaaga actcgccatt tttgtttcgg tcgtagaaag cggcagcttt
     3481 agccgggcag cggaacaatt agggcaagca aactcagcgg taagccgggc agtgaaaaag
     3541 ctggagatga aacttggcgt tagcctgctt aatcggacca cgcgacaact tagcctgacg
     3601 gaagaaggcc agcgttattt tcgtcgcgta cagtcaattt tgcaggagat ggcagcggca
     3661 gaatcagaaa ttatggagac gcgtaataca ccgcgtggac tgttacgcat cgatgccgca
     3721 actccagtgg tgctgcactt tctgatgccg ttaattaagc ctttccgtga acgctatccg
     3781 gaagttactt tatcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtg
     3841 gatgtcgcga tacgcgctgg tacgttaacg gattccagct tacgtgccag gccgttattt
     3901 aacagttatc gaaaaattat cgcctccccc gattatattt cccgctacgg gaagccagaa
     3961 acgatcgacg atttaaagca acatgtttgc ctgggattca ctgaacccgc ttccctcaat
     4021 acctggccga tagcctgtag tgatggacaa ttacatgagg tgaagtacgg tttgtcatcc
     4081 aatagtgggg aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc
     4141 gactacatga tcgacaaaga aatcgttcgc ggagaattgg tggagttaat ggcagataaa
     4201 gtgttgccag tggaaatgcc attcagtgca gtctattaca gcgaccgtgc ggtaagtacg
     4261 cgcatccggg cttttatcga tttccttagc gagcatgtaa aaacagctcc cggaggagct
     4321 gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg
     4381 caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta
     4441 agattacttt ccaggttttc acgtttagta gaagaaggaa ttactgagta accttccccc
     4501 atagcccacg ccagaatcac ttgtgccgga gtcgcattgt gtttagctgc gatacgagca
     4561 ataacctcat ctttcaaggc tttcccataa gccagcgtca tataggaagt aatatggatg
     4621 ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga gagttcaatc
     4681 tggttagtag cgatgttttc agcgccaata acagcaatcg ccttttccat caatgggatc
     4741 gtgaagttgg aaataccgat ctcacgcgtc agcccttgtt ttttggcttc cagcagcgcc
     4801 tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat tagcgtcaga
     4861 tcaacataat cggtacgcaa tttttgcagg ctctctttca gacttgggat caatttgtct
     4921 ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg tggcacgcca
     4981 ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt
     5041 gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc gtctttcaga
     5101 cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt
     5161 cgttaactga ggtgattatg ggcttcagac tgttgaggaa aaagagcgta aaatgcagag
     5221 gatttttgcg attctggcaa taatagatat ccagacgaaa aaaaacctcg agcgttaact
     5281 cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac
     5341 aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt
     5401 ttatgaccgg cttactgctt aatttgatgc ctggcagttt atgaatcact tcgtgattca
     5461 cccttcgggc cgttgcttcg caacgttcaa aagctttcgc ttttgtccta ctctcttatc
     5521 gagtagtgaa ctgcctcaaa gatcagtgcc attcttcg
//
LOCUS       DAZFRJ010000241         5478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000241 DAZFRJ010000000
VERSION     DAZFRJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.243"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..984)
                     /gene="phoE"
                     /locus_tag="ACHKUN_004305"
     CDS             complement(<1..984)
                     /gene="phoE"
                     /locus_tag="ACHKUN_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6662440.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQ"
     gene            1272..2375
                     /gene="proB"
                     /locus_tag="ACHKUN_004306"
     CDS             1272..2375
                     /gene="proB"
                     /locus_tag="ACHKUN_004306"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO6662441.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            2387..3640
                     /gene="proA"
                     /locus_tag="ACHKUN_004307"
     CDS             2387..3640
                     /gene="proA"
                     /locus_tag="ACHKUN_004307"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO6662442.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            3755..3830
                     /locus_tag="ACHKUN_004308"
     tRNA            3755..3830
                     /locus_tag="ACHKUN_004308"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3788..3790,aa:Thr,seq:cgt)
     gene            3994..5202
                     /locus_tag="ACHKUN_004309"
     CDS             3994..5202
                     /locus_tag="ACHKUN_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6662443.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDDAKKKLAEGIDPSLAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKPLVLLNVLRRIESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSTQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGRVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADYINANHNS
                     CISLLVNATK"
BASE COUNT         1382 a         1298 c         1422 g         1376 t
ORIGIN      
        1 ttggttgatt ttataatcaa caaacgctga catatttttg ttgaaataat atgtagcccc
       61 gacatcgata taattgacca gatcttcatc accgatacct tcaatatctt tccctttcga
      121 taagacataa cccagcgatg gacgcagacc aaagtcaaac tggtattgag cgaccgcttc
      181 aaagttctgt gtcttattgg caaagccgcc agttattggc gtcattttgc gtgtttcaga
      241 ataaaaagtt gccagataaa aattattggc atcgtatttc agacctgtag cccatgcttc
      301 tgcacgcttg cctgtgccac ggctttgcag gttctgctcg ttggtgcgat ctgagttggt
      361 ataggcccca ctaatggcga aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa
      421 gccatcgccg ttttgctttt taacgtcgcg gttttcgttt ttcccttgat attgcagggt
      481 taagttcagg ccatcgataa cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct
      541 ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa tcgccaccaa attccgggaa
      601 catatcggtc caggcttcca cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga
      661 acccaaatct ttatatttca acccggcaaa agcgagacgc gttttttgct gtgcggtatc
      721 actctccgct ttatttccgg caaactccgc ttcccaccga ccataaccag tcagttgatc
      781 gttaatttgt gtttcacctt taaaaccaaa acggatataa ctctggtcgc catctttact
      841 ggcgttatca ctcatataat gcatggcttt aactttgcca tagacatcca gtttattacc
      901 gtctttatta tatatttctg cagcctgtac agatgcagat gccacaatgc ccatcaccac
      961 taatgccaga gtgctctttt tcattttcat tcctgatttt aattaacgcg cgaatattca
     1021 gcgggagagc cccgttgaaa acaggaaagt ttttaacctg agattgttaa agatatatta
     1081 cagattaata atattcttaa aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca
     1141 actgccgcta ggcttgctga tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt
     1201 ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat catttttcct aaaattgaat
     1261 ggcagagaat catgagtgac agccagacgc tggtggtaaa actcggcacc agtgtgctaa
     1321 caggcggatc gcgccgtctg aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc
     1381 agttacatgc cgccgggcat cggattgtta ttgtgacgtc gggcgcgatc gccgccggac
     1441 gtgagcacct gggttacccg gaactgccag cgactatcgc ctcgaaacaa ctgctggcag
     1501 cggtagggca gagtcgactg attcaactgt gggaacagct gttttcgatt tatggcattc
     1561 acgtcgggca aatgctgctg acccgtgctg atatggaaga ccgtgaacgc ttcctgaacg
     1621 cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt tccggtaatc aatgagaacg
     1681 atgctgtcgc tacggcagag attaaagtcg gcgataacga taacctttct gcgctggcgg
     1741 cgattctggc gggtgccgat aaactgttgc tgctgaccga tcaaaaaggt ttgtataccg
     1801 ctgacccgcg cagcaatccg caggcagaac tgattaaaga tgtttacggc attgatgacg
     1861 cactgcgcgc gattgccggt gacagcgttt caggcctcgg aactggcggc atgagtacca
     1921 aattgcaggc tgctgacgtg gcttgccgtg cgggtatcga caccattatt gccgcgggca
     1981 gcaagccggg cgttattggt gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg
     2041 cccaggcgac tccgcttgaa aaccgtaaac gctggatttt cggtgcgccg ccggcgggtg
     2101 aaatcacggt agatgaaggg gcaactgccg ccattcttga acgcggcagc tccctgttgc
     2161 cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg tgaagtcatc cgcatttgca
     2221 acctcgaagg tcgcgatatc gcccacggcg tcagtcgtta caacagcgat gcattacgcc
     2281 gtattgccgg gcaccactcg caagaaattg atgcaatact gggatatgaa tacggcccgg
     2341 ttgccgttca ccgtgatgac atgattaccc gttaaggagc aggctgatgc tggaacaaat
     2401 gggcattgcc gcgaagcaag cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa
     2461 tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa agcgaaatca tcctcaacgc
     2521 taacgcccag gatgttgctg acgcgcgtgc caatggcctt ggcgaagcga tgcttgaccg
     2581 tctggcactg acgcccgcac ggctgaaagg cattgccgat gatgtgcgcc aggtgtgtaa
     2641 cctcgccgat ccggtggggc aggtaatcga tggcagcgta ctggacagcg gcctgcgtct
     2701 tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt tatgaagcgc gcccgaacgt
     2761 gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat gcggtgatcc tgcgcggtgg
     2821 caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg attcaggacg ccctgaaatc
     2881 ctgcggctta ccggcgggtg ccgtgcaggc gattgataat cctgaccgtg cgctggtcag
     2941 tgaaatgctg cgtatggata aatacatcga catgctgatc ccgcgtggtg gcgctggttt
     3001 gcataaactg tgccgtgaac agtcgacaat cccggtgatc acaggtggta taggcgtatg
     3061 ccatatttac gttgatgaaa gtgtagagat cgctgaagca ttaaaagtga tcgtcaacgc
     3121 gaaaactcag cgtccgagca catgtaatac ggttgaaacg ttgctggtga ataaaaacat
     3181 cgccgatagc ttcctgcccg cattaagcaa acaaatggcg gaaagcggcg tgacattaca
     3241 cgcagatgca gctgcactgg cgcagttgca ggcaggccct gcgaaggtgg ttgctgttaa
     3301 agccgaagag tatgacgatg agtttctgtc attagatttg aacgtcaaaa tcgtcagcga
     3361 tcttgacgat gccatcgccc atattcgtga acacggcaca caacactccg atgcgatcct
     3421 gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa gtggattcgt ccgctgttta
     3481 cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt ggtctgggtg cggaagtggc
     3541 ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg ctggaagcac tgaccactta
     3601 caagtggatc ggcattggtg attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa
     3661 agtagccatt tgattcacaa ggccattgac gcatcgcccg gttagtttta accttgtcca
     3721 ccgtgattca cgttcgtgaa catgtccttt cagggccgat atagctcagt tggtagagca
     3781 gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt atcggcacca tttcaaactc
     3841 ttcccaagtc taccgaaatc aactaaaagc tcgtatactg cggtttctgg cccgtatctt
     3901 atctcctgtt atcaactaga ctcaaccgaa atcaagtcgc agttgggggc ataagtgggg
     3961 gcattttgtg ttcggtctag ggagatgccc ccaatgaaac ttaatgcgcg acaggtagac
     4021 gcagctaaac ccagagagaa agcctataag ctggcagatg gtgctggttt gtatcttgag
     4081 gttgttccct ctggttccag atactggcgg atgaaatatc gcttcaatgg aaaagagaag
     4141 cgtatggcct ttggtgtcta tccggcagtg tcccttgcac aagcgagggc actgcgtgac
     4201 gacgccaaga aaaagcttgc cgaaggtatc gatccatcgc ttgccaagaa agaagaaaag
     4261 ctggttcgag atgtgcagct aaataatacg tttcaggctg tggcacttga atggcatgga
     4321 acgaaggtga gccgatggtc agaaggttat gcctcggaca ttatcgaagc ctttaataaa
     4381 gatatttttc cctatattgg ccaacaaccg gtgaatgaaa tcaaaccgct ggttctgctt
     4441 aatgtgctgc gtcgaattga aagccgtggc gcgacagaga aggccaagaa ggttcgccag
     4501 cgttgcagcg aagtctttcg ttacgccatc gtaaccggtc gtgcggaata caatcctgca
     4561 gcggatctta ccagcgcgat gtcagggcat gaatcgaagc attatccctt ccttactgtt
     4621 gaggagttac cagacttttt taaagctctc gcaggctaca caggaagccc gttagttgtt
     4681 cttgcggcac gtctgctgat cctcacggga gttcgcactg gcgaactccg aggtgctttc
     4741 tggagtgagt ttgatcttga aaaagcggtg tgggagatac ctgccgagcg tatgaagatg
     4801 aaacggcctc accttgtccc cctctctacc caagcgctgg aaatcgtaca gcaactcaaa
     4861 gtgatgtcag ggcaatatcc actggtgttc ccgggacgta atgatccccg caagacgatg
     4921 agtgaagcga gtattaatca ggtttttaag cggattggat atacggggag ggtaacgggg
     4981 catggtttcc gtcacacgat gagtacgatt ttgcatgagg aaggtttcaa tacagcgtgg
     5041 attgaaaccc agctggctca cgttgataaa aatgcgattc gtgggacgta taaccatgcg
     5101 ttgtatttgg aaggacgtag ggagatgatg cagtggtatg cggactatat aaacgctaat
     5161 cataattcat gtatttcttt gctggtgaac gctacaaagt agatttgacg tgatgatagg
     5221 attgattagg aaaatgacct aaaaccgtat attttgatac ttaagtttaa acattagccc
     5281 tatatccgat cgccctttgt tacctacact tctatctttt ttaggttcta gggctgaaag
     5341 atttgaagat aagtttataa atatcaatag attacatgat tgtgttggtt cttgggtagc
     5401 cgtaacgcct agggcggtag cgtacttaag atgagtataa aaaatgaaca gtaactatat
     5461 gccattcaca caacgaga
//
LOCUS       DAZFRJ010000242         5473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000242 DAZFRJ010000000
VERSION     DAZFRJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.244"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            154..489
                     /gene="eutS"
                     /locus_tag="ACHKUN_004310"
     CDS             154..489
                     /gene="eutS"
                     /locus_tag="ACHKUN_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO6662444.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            502..981
                     /gene="eutP"
                     /locus_tag="ACHKUN_004311"
     CDS             502..981
                     /gene="eutP"
                     /locus_tag="ACHKUN_004311"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO6662445.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            956..1657
                     /gene="eutQ"
                     /locus_tag="ACHKUN_004312"
     CDS             956..1657
                     /gene="eutQ"
                     /locus_tag="ACHKUN_004312"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO6662446.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1654..2457
                     /gene="eutT"
                     /locus_tag="ACHKUN_004313"
     CDS             1654..2457
                     /gene="eutT"
                     /locus_tag="ACHKUN_004313"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO6662447.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2454..3470
                     /gene="pta"
                     /locus_tag="ACHKUN_004314"
     CDS             2454..3470
                     /gene="pta"
                     /locus_tag="ACHKUN_004314"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6662448.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3509..3802
                     /gene="eutM"
                     /locus_tag="ACHKUN_004315"
     CDS             3509..3802
                     /gene="eutM"
                     /locus_tag="ACHKUN_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO6662449.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3909..4196
                     /gene="eutN"
                     /locus_tag="ACHKUN_004316"
     CDS             3909..4196
                     /gene="eutN"
                     /locus_tag="ACHKUN_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO6662450.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4208..>5473
                     /locus_tag="ACHKUN_004317"
     CDS             4208..>5473
                     /locus_tag="ACHKUN_004317"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO6662451.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPC"
BASE COUNT         1281 a         1442 c         1613 g         1137 t
ORIGIN      
        1 tcatgcgttg catatgaaag tttatgcacc acagcgaata tctctccatt cttagtgatc
       61 tacctcacct tttaaacgcg cttgccgaat tttgttattt actctgacga aaaattgtca
      121 cgatacacga aagtttttca caggcggcga ctcatggata aagaacgcat cattcaggaa
      181 tttgtgcccg gcaaacaggt cacactggcg catctcatcg cgcatcccgg tgaggaactg
      241 gcgaagaaaa tcggcgttcc ggatgcgggc gcaattggca ttatgacgct aactcccggc
      301 gaaacggcga tgatcgccgg tgatttagcg ctgaaagccg ctgatgtgca tatcggtttt
      361 ctcgaccgct tcagcggtgc attggtgatt tatggctcgg tgggcgcagt agaagaagcg
      421 ttatcacaaa ccgtcagcgg tttaggtcgc ttattaaatt atacgctttg tgaaatgacc
      481 aaaagttaat acgaggcatt catgaaacga attgcgtttg tgggtaccgt cggggcagga
      541 aaaacaacgt tatttaatgc gttgcagggg aattataccc tcgccagaaa aacacaggcc
      601 gtggaattta atgataaggg cgatattgat acgccgggtg aatattttag ccatccccgc
      661 tggtatcacg ccttaattac cacgctacag gatgtagaca tgctgatcta tgtccatggc
      721 gcaaatgacc cggaaagtcg gttgcctgcc gggttgctgg atattggcgt cagcaaacgg
      781 caaatcgccg tcatcagtaa aacggacatg ccagatgctg acgtcgccgc aacgcgaaag
      841 ttattgcttg agacgggttt cgaagagccg attttcgagc taaacagcca cgacccgcaa
      901 agcgtgcagc aactggtgga ttatctggca tctctcacca aacaggagga agcaggtgaa
      961 aaaactcatc acagcgaatg atattcgtga agcacacgcg cgcggcgaac aggcaatgtc
     1021 cgtggttctg cgcgccagca ttattacccc ggaagcccgt gaagtggcgg atctactggg
     1081 ttttaccatc accgaatgtg atgaatcgat cccggtaacg gcgtccgtgc ccgccagcgt
     1141 gccagccgac aaaaccgaaa gccagcgcat ccgcgaaacc atcattgccc agctgccgga
     1201 aggccagttt accgaaagcc tggtcgcaca gctgatggaa aaagtgatga aggaaaaaca
     1261 gtcgctggag cagggcgcaa tgcagccgag cttcaaatcg gtgaccggca aaggcggcat
     1321 caaagtgatt gatggcagca gcgtcaagtt tggtcgtttc gacggcgcag agccgcattg
     1381 cgtgggcttg actgatcttg tgaccggaga cgacggcagc agcatggccg ctggttttat
     1441 gcagtgggag aacgcattct tcccgtggac cctgaactac gacgaaatcg acatggtgct
     1501 ggaaggggag ctgcatgttc gccacgaagg cgaaaccatg atcgccaaag cgggtgacgt
     1561 gatgtttatc ccgaaaggct ccagtatcga atttggtacg acatccagcg tgaaattcct
     1621 gtatgtcgcc tggccggcta actggcaatc cctatgaaag atttcatcac cgaagcatgg
     1681 ctaagagcga accatacgct cagcgaaggc gcagagatcc atctccctgc ggacagtcgc
     1741 ctgacgccct ctgcccggga gttactggaa agccgccatc tgcgcatcaa gtttattgac
     1801 gagcagggcc gcctgtttgt tgacgatgaa cagcagcagc cgcagcccgt tcatgggctg
     1861 accagtagcg atgaacatcc gcaggcgtgc tgcgaactgt gccgccagcc ggtggcaaaa
     1921 aagccggata cgctgactca cctgtcggcg gaaaaaatgg tcgccaaaag cgatccgcgt
     1981 ctgggttttc gcgcggttct cgacagcacc attgcgctgg cagtgtggct gcaaattgaa
     2041 ctggcggaac cgtggcagcc gtggctggcg gatatccgtt cgcgtcttgg caacattatg
     2101 cgcgccgatg cgctgggtga accgctgggc tgccaggcga ttgtcgggct ttctgacgaa
     2161 gatctgcatc gactttctca ccagccgctg cgctatctcg accacgatca tctggtgccg
     2221 gaagccagcc acgggcgcga tgcggcgttg ctcaatctgc tacgtaccaa agtgcgggaa
     2281 accgaaaccg tagcggcgca ggtgtttatc acccgcagct ttgaagtatt acgcccggac
     2341 attttgcagg cgctgaaccg cctttccagc acggtctacg tgatgatgat cctgagcgta
     2401 acgaagcagc cgctaacggt gaagcagatc caacaacgac tgggggaaac gcaatgatta
     2461 ttgaacgttg tcgtgaactg gcgttgcgag cgcccgccag agtggttttt ccggatgcgt
     2521 tagaccaacg tgtgctgaaa gccgcgcaat atttacatca acaaggtctg gcaacgccca
     2581 ttctggtcgc caatccgttt gaacttcgtc agtttgcgct cagtcacggc gtggcgatgg
     2641 acgggctaca ggtgattgat ccgcatggca acctcgcaat gcgggaagaa tttgctcatc
     2701 gctggctggc ccgcgcgggc gaaaaaacgc cgccggatgc gctggaaaaa cttaccgacc
     2761 ctctgatgtt cgccgcagca atggtcagcg ccggtaaagc ggatgtctgt atcgcgggca
     2821 acctctcttc cacggcaaat gtgctgcgtg ccggattacg cattatcggc ttgcagccag
     2881 gctgtaaaac gctctcttcc attttcctga tgttgccaca gtacagcggc ccggcgttgg
     2941 gctttgccga ttgcagcgtg gtgccacagc cgacggcggc gcagctggcg gatatcgcgc
     3001 ttgccagtgc cgaaacctgg cgcgccatca ccggagaaga gccgcgcgtg gcgatgctgt
     3061 cgttttccag caatggtagc gcccgtcacc cctgtgttgc taacgtccag caggcgacag
     3121 aaatcgtccg tgagcgcgca ccaaagctgg tggtggatgg cgagttacag tttgacgccg
     3181 ccttcgtgcc ggaagtggcg gcgcaaaaag cgcctgccag cccgctacag ggcaaggcca
     3241 atgtgatggt ttttccgtcg ctggaagccg gaaatattgg ttacaaaatc gcacaacgac
     3301 tcggcggata tcgtgccgtc gggccactga tacaaggact tgccgcgccg atgcacgatc
     3361 tctctcgtgg ttgtagcgtg caggaaatta tcgagctggc gctggtggca gctgtgccgc
     3421 gtcagacaga agtgaaccgc gaaagcagtt tacaaacact ggttgaatga acggtcccgt
     3481 tctggaccct gataaagagg aaagcacaat ggaagcatta ggaatgatcg aaacccgggg
     3541 cctggttgca ctgattgagg cttctgacgc gatggtgaaa gctgcgcgcg tcaagctggt
     3601 tggtgttaag cagattggcg gcggcctgtg tactgccatg gttcgtggtg atgttgcagc
     3661 ctgtaaagcc gcgaccgatg caggtgccgc agcggcacag cgtatcggcg agctggtatc
     3721 tgtacacgtt atcccgcgtc cgcacggcga cctggaagaa gtcttcccga tcggcctgaa
     3781 aggcgatagc agcaacctgt aatcaccctg tgtgacgtcg attcgggcgt cacgctctct
     3841 cctttttcat tttaccttct gcggagggta gggggagctt ttacctgaaa tacgccacgg
     3901 aggcgggtat gaaactggca gtcgtcactg gacaaattgt ttgtaccgta cgccatcacg
     3961 gactggcgca tgacaaattg ctgatggtgg aaatgattga tccacaaggt aatcctgacg
     4021 ggcaatgcgc cgtcgccatc gacaatattg gcgcgggaac cggggagtgg gtgttactgg
     4081 tgagtggcag ttccgcccgc caggcgcata aaagcgaaac gtcaccggtc gatctgtgcg
     4141 tgattggcat tgtcgatgag gtggtgtctg gcggtcaggt aattttccac aaataaggca
     4201 gaacatcatg aatcaacagg atattgaaca ggtggtgaaa gcggtactgc tgaaaatgca
     4261 aagcagtgac acgccgtccg ccgccgttca tgagatgggc gttttcgcgt ccctggatga
     4321 cgccgttgcg gcagccaaag tcgcccagca agggttaaaa agcgtggcaa tgcgccagtt
     4381 agccattgct gccattcgtg aagcaggcga aaaacacgcc agagatttag cggaacttgc
     4441 cgtcagtgaa accggcatgg ggcgcgttga agataaattt gcaaaaaacg tcgctcaggc
     4501 gcgcggcaca ccaggcgttg agtgcctctc tccgcaagtg ctgactggcg acaacggcct
     4561 gaccctaatt gaaaacgcac cctggggcgt ggtggcttcg gtgacgcctt ccactaaccc
     4621 ggcggcaacc gtaattaaca acgccatcag cctgattgcc gcgggcaaca gcgtcatttt
     4681 tgccccgcat ccggcggcga aaaaagtctc ccagcgggcg attacgctgc tcaaccaggc
     4741 gattgttgcc gcaggtgggc cggaaaactt actggttact gtggcaaatc cggatatcga
     4801 aaccgcgcaa cgcttgttca agtttccggg tatcggcctg ctggtggtaa ccggcggcga
     4861 agcggtagta gaagcggcgc gtaaacacac caataaacgt ctgattgccg caggcgctgg
     4921 caacccgccg gtagtggtgg atgaaaccgc cgacctcgcc cgtgccgctc agtccatcgt
     4981 caaaggcgct tctttcgata acaacatcat ttgtgccgac gaaaaggtac tgattgttgt
     5041 tgatagcgta gccgatgaac tgatgcgtct gatggaaggc cagcacgcgg tgaaactgac
     5101 cgcagaacag gcgcagcagc tgcaaccggt gttgctgaaa aatatcgacg agcgcggaaa
     5161 aggcaccgtc agccgtgact gggttggtcg cgatgcggcg aaaatcgcgg cggcaatcgg
     5221 cctgaacgtg ccgcaagaaa cgcgtttgct gtttgtggaa accactgcag aacatccgtt
     5281 tgccgtgacc gaactgatga tgccggtgct gcccgtcgtg cgcgtcgcca acgtggcgga
     5341 tgccattgcg ctggcggtga aactggaggg cggttgccac cacacggcgg caatgcactc
     5401 gcgcaatatc gaaaacatga accagatggc gaacgccatt gataccagca ttttcgttaa
     5461 gaacggaccg tgc
//
LOCUS       DAZFRJ010000243         5403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000243 DAZFRJ010000000
VERSION     DAZFRJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.245"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..407
                     /locus_tag="ACHKUN_004318"
     CDS             39..407
                     /locus_tag="ACHKUN_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO6662452.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            480..644
                     /locus_tag="ACHKUN_004319"
     CDS             480..644
                     /locus_tag="ACHKUN_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO6662453.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            613..777
                     /gene="kil"
                     /locus_tag="ACHKUN_004320"
     CDS             613..777
                     /gene="kil"
                     /locus_tag="ACHKUN_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO6662454.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            832..1128
                     /locus_tag="ACHKUN_004321"
     CDS             832..1128
                     /locus_tag="ACHKUN_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6662455.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1134..1919
                     /gene="bet"
                     /locus_tag="ACHKUN_004322"
     CDS             1134..1919
                     /gene="bet"
                     /locus_tag="ACHKUN_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO6662456.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1916..2593
                     /locus_tag="ACHKUN_004323"
     CDS             1916..2593
                     /locus_tag="ACHKUN_004323"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6662457.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2593..2775
                     /locus_tag="ACHKUN_004324"
     CDS             2593..2775
                     /locus_tag="ACHKUN_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO6662458.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2748..2939
                     /locus_tag="ACHKUN_004325"
     CDS             2748..2939
                     /locus_tag="ACHKUN_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO6662459.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            3016..3231
                     /locus_tag="ACHKUN_004326"
     CDS             3016..3231
                     /locus_tag="ACHKUN_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6662460.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3330..3551
                     /locus_tag="ACHKUN_004327"
     CDS             3330..3551
                     /locus_tag="ACHKUN_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6662461.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            3548..3853
                     /locus_tag="ACHKUN_004328"
                     /pseudo
     CDS             3548..3853
                     /locus_tag="ACHKUN_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            4150..4500
                     /locus_tag="ACHKUN_004329"
     CDS             4150..4500
                     /locus_tag="ACHKUN_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6662462.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            4575..4817
                     /locus_tag="ACHKUN_004330"
     CDS             4575..4817
                     /locus_tag="ACHKUN_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6662463.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            4936..5280
                     /locus_tag="ACHKUN_004331"
     CDS             4936..5280
                     /locus_tag="ACHKUN_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662464.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1558 a         1219 c         1366 g         1260 t
ORIGIN      
        1 aatgtgaggg cgattttttt ctgtgaggat atgaacagat gtcaaacatc aaaaaataca
       61 tcattgatta cgactggaaa acatcaatag aaattgaaat cgaccatgac ttaatgacag
      121 aggaaaaact tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac
      181 acggctctgt attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa
      241 tttcaagcga cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg
      301 gtcaggaagg atggcctcca atggatggta gcgaaggaat aagaattacc gatatcgata
      361 catcaggaat atttgatcca gatgatatga ctatcaaagc cgcctgagcg cggcgttacc
      421 gcataccaat tacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca
      481 tgcaatatgc cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac
      541 gaattacccg taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag
      601 gagtcccgaa aaatggatca aacaattatg gctatccaga ctaaattcac tatcgccact
      661 tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta
      721 atacagaaac tgagatcaag caaaagcatt cactaccccc ctttcctgtt ttcctaatca
      781 gcctggcatt tcgcgggcga tattttcaca gccattttca ggagttcagc catgaacgct
      841 tattacattc aggatcgtct tgaggctcag agctgggcgc gtcactacca gcagatcgcc
      901 cgtgaagaga aagaggcaga actggcagac gacatggaaa aaggcctgcc ccagcacctg
      961 tttgaatcgc tatgcatcga tcatttgcaa cgccacgggg ccagcaaaaa agccattacc
     1021 cgtgcgtttg atgacgatgt tgagtttcag gaacgcatgg cagaacacat ccggtacatg
     1081 gttgaaacca ttgctcacca ccaggttgat attgattcag aggtataaaa cggatgagta
     1141 cagcactcgc aacgctggct gggaagctgg ctgaacgtgt cggcatggat tctgtcgacc
     1201 cacaggaact gatcaccact cttcgccaga cggcatttaa aggtgatgcc agcgatgcgc
     1261 agttcatcgc attgctgatc gtcgccaacc agtacggcct taatccgtgg acgaaagaaa
     1321 tttacgcctt ccctgataag cagaacggca tcgttccggt ggtgggcgtt gatggctggt
     1381 cccgcatcat caacgaaaac cagcagtttg atggcatgga ctttgagcag gacaatgaat
     1441 cctgcacatg ccggatttac cgcaaggacc gtaatcatcc gatctgcgtt accgagtgga
     1501 tggatgaatg ccgccgcgaa ccattcaaaa cccgcgaagg cagagaaatc acggggccgt
     1561 ggcagtcgca tcccaaacgg atgttacgtc ataaagccat gattcagtgt gcccgtctcg
     1621 ccttcggatt tgctggtatc tatgacaagg atgaagccga gcgcattgtc gaaaataccg
     1681 catacactgc agaacgtcag ccggaacgcg acatcactcc ggttaacgat gaaaccatgc
     1741 aggaaattaa cactctgctg atcgccctgg ataaaacatg ggatgacgac ttattgccgc
     1801 tctgttccca gatatttcgc cgcgacattc gtgcatcgtc agaactgaca caggccgaag
     1861 cagtaaaagc tcttggattc ctgaaacaaa aagccactga acagaaggtg gcagcatgac
     1921 accggacatt atcctgcagc gtactgggat cgacgtgaga gctgtcgaac agggagatga
     1981 tgcgtggcac aaattacggc tcggcgtcat cacagcttca gaaattcaca acgtaatagc
     2041 aaaaccccga tcaggaaaga agtggcctga catgaaaatg tcctacttcc acaccctgct
     2101 ggctgaggtt tgcaccggtg tggctccgga agttaacgct aaggctctgg cctggggaaa
     2161 acagtacgag aacgacgcca gaaccctctt tgagttcact tccggcgtga atgttactga
     2221 atccccgatc atctatcgcg acgaaagtat gcgcaccgcc tgctctcccg atggtttatg
     2281 cagtgacggc aacggccttg agctgaaatg cccgtttacc tcccgggatt tcatgaagtt
     2341 ccggctcggt ggtttcgagg ccataaaatc ggcttacatg gcccaggtgc agtacagcat
     2401 gtgggtgaca cgaaaagatg cctggtactt tgccaactat gacccgcgca tgaagcgtga
     2461 aggcctgcat tatgtcgtga ttgagcggaa tgaaaagtac atggcgagtt ttgacgagat
     2521 ggtgccggag ttcatcgaaa aaatggacga ggcactggct gaaattggtt ttgtatatgg
     2581 ggagcaatgg taatgaagca tcctcacgat aatatccggg taggcacgat cactttcgtc
     2641 tactccgtta caaagcgagg ctgggtattt cccggccttt ctgttatccg aaatccactg
     2701 aaagcacagc ggctggctga agagataaat aataaacgag gggctgtatg cacaaagcat
     2761 ctcctgttga attaagaacg agtatcggga tggcacatag cctcgctcaa attggagtca
     2821 ggtttgtgcc aataccagta gaaacagacg aagaatttca tacgttagcc acatcccttt
     2881 cacaaaagct ggaaatgatg gcggcgaaag cagaagcaaa cgagagagac ccggcatgac
     2941 aacaacagaa tgcatttttc tggcagcagg cttcatattc tgtgtgctta tgcttgccga
     3001 catgggactt gttcaatgac acctcagcag gaaaacgccc ttcgcagcat tgcccgtcag
     3061 gctaattctg aaatcaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt
     3121 tgccgtagcg tactgaagaa gcaccgcgaa acggtaacgc taatgggatt cacaccgact
     3181 catttaagtc tggcgatcgg catgttaaac ggcgtcttta aggagcgatg aacatgaaaa
     3241 gcaaaatcat cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta
     3301 ttaaccaaca attcagggat taatggaaga tggcagacat cattgattca gcatcagaaa
     3361 ttgaagaatt acagcgcaac acagcaataa aaatgcaccg cctgaaccac caggctatat
     3421 ctgccactca ttgttgtgag tgtggcgatc cgatagatga actaagacgt ctggccgttc
     3481 agggttgtcg gacttgtgca agttgccaag aggatctgga gcttatcagt aaacagagag
     3541 gttcgaagtg agcgaaatta actctcaggc actgcgtgaa gcggcagagc aggcaatgca
     3601 tgacgactgg ggatttgatg cggacctttt ccatgagctg gtaacaccat cgattgtgct
     3661 ggcactgctg gatgaacggg aaagaaacca gcaatacatc aaacgccgcg accaggagaa
     3721 cgaggaaatt gcgctaacgg tagggaagct gcgtgttgag cttgaggaag caaaatcaaa
     3781 actcaacgag cagcgtgagt attacgaagg tgttatctcg gatgggagta aacgtattgc
     3841 tgaactggag aaaagcgaag agcaactcat taacgagcgt gaccatgctg agtctgcttt
     3901 agctgatatg tacttcgcaa caaccgggga taggcctgag tggagtaact gtttcagttt
     3961 ttcagatgct gtcgatgccg tagttgacag aattgctgat ttagaagcca aacagccatc
     4021 gccagtagta ccggaagaaa aaccaatgcc taaccctctt agcatgtaca cagttgatgc
     4081 tgttgcagct attgcagagg tgagaggctg gaacgcctgc cgtgcagcca tgcttaaggg
     4141 agataaatca tgattaatcg aaccaaactg gagcacatcc tcgagtatgc caggcagcag
     4201 aggcgctttg gccagctttg taaaattctg ccaggagata tggttgaaat cgtggagatt
     4261 gccatgcgta agactggcaa ctctccggta agtccgggtg gttggataag ctgtagtgag
     4321 cgaatgcccg ctcaagatga ttggatttta atttattcaa agcacggtga gtatatggca
     4381 ggacaggtac aagaggaata cgtggagttg agcgacggca ctttatcgtg gttagggaac
     4441 gccttgtact ggatgctgct accagaaccg ccgcagggag tgaatgatga attggcctga
     4501 agcattcacc gctgtaggag ttgcaatcgc ggtggcattt attctgtatt cgcttttccg
     4561 ctggggataa aggaatgttc gctctgattc aacgtggtca gatatacacc gatagcgccg
     4621 gctacccgat aaaaattctt cgctgcataa acaacactgt gttgtacaga agaatggatg
     4681 ggcgaacaca gtcggtaaaa ataaacgatt ttaatgaact gtttgaacgg atcgatcacc
     4741 aggaataccg acaaattctg gctgaaacag agcaggagaa ccatctgaaa aaattacgcg
     4801 ccatgcaaag gagataaacc ggtaaaggtg ttcgcgataa aggtgaatat cggcaatgaa
     4861 taacaatcct cgcactcgcg gggatttctt ttatctgaac tcgctacggc gggttttgtt
     4921 ttatggagac aagaaatgtc agatttggct atgaaggttt tgaaatggca atcgactggc
     4981 gatgttggca tcagtagcgc aactcttgcc tcaatcgcat gtggactgaa aaagaatatc
     5041 tatggtcatc acttcggcgc tccacatgac gcagcagact tccggcgatg cgttgcactt
     5101 gttgagcaga ttccagaaat cagagattca ttcgacaagg ttgcaaagcg cgttccggca
     5161 ttcaaaggca tcctcaacga atgggattcc ctcgtcgctc tgttgaagtc tgaaatgaag
     5221 atacacggaa acaaagcacc agagacttac agaagaatta gcgagctacg caaggactaa
     5281 ccacagcctc acactcaatg aggcctgttc atttctcaag atatccagac ctaccattgc
     5341 cgcatcaatg cggcttttct tgcgtgtaat tgcggagact ttgcgatgta cttgacactt
     5401 cag
//
LOCUS       DAZFRJ010000244         5391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000244 DAZFRJ010000000
VERSION     DAZFRJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.246"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..518
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUN_004332"
     CDS             <1..518
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUN_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO6662465.1"
                     /translation="TISRDTPVDYSQPMADVYIRGDITAPQSCEINNLQPVYFDFKEI
                     PAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNS
                     NVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPASLTGARPSPGQFT
                     ATATITVEIVR"
     gene            766..1806
                     /gene="pstS"
                     /locus_tag="ACHKUN_004333"
     CDS             766..1806
                     /gene="pstS"
                     /locus_tag="ACHKUN_004333"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO6662466.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1893..2852
                     /gene="pstC"
                     /locus_tag="ACHKUN_004334"
     CDS             1893..2852
                     /gene="pstC"
                     /locus_tag="ACHKUN_004334"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO6662467.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2852..3742
                     /gene="pstA"
                     /locus_tag="ACHKUN_004335"
     CDS             2852..3742
                     /gene="pstA"
                     /locus_tag="ACHKUN_004335"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO6662468.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3833..4606
                     /gene="pstB"
                     /locus_tag="ACHKUN_004336"
     CDS             3833..4606
                     /gene="pstB"
                     /locus_tag="ACHKUN_004336"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO6662469.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4621..5346
                     /gene="phoU"
                     /locus_tag="ACHKUN_004337"
     CDS             4621..5346
                     /gene="phoU"
                     /locus_tag="ACHKUN_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO6662470.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1301 a         1318 c         1442 g         1330 t
ORIGIN      
        1 gaacaattag ccgtgacacc ccggtggatt actcacagcc gatggctgat gtttatattc
       61 ggggcgatat tacagctccg caaagttgcg aaataaataa tttacagcca gtttattttg
      121 attttaaaga aataccagct gcagattttt catctgttgt tggaagtgcg gtaacaacac
      181 ataaaattac taaaacggtc actattgagt gtgaaaacct ggggatactg aataccgatg
      241 atatcagtac gtctttttat gctaccgaac ccaatacgga caattcaatg gtcgtaactt
      301 caaactctaa cgtggggata aaaatttacg acaaaaataa taaggaaatc aaagtgaacg
      361 gtggtgagtt gccaacagac atgggtaaat caacagtgta tggtgaaaaa tcaggtagtg
      421 taactttttc agctgctcct gcgagcctta ctggagctcg cccatccccg gggcaattta
      481 ctgcaacagc gacgataact gttgagattg tgcgctaata ggtctgacaa cgattgttca
      541 tcacatcgtt gtcatctcac ccatttcttt atcactttta aatgcatcat gacaaaaaga
      601 tgacatgtgg gattttcatc ttcacattta aaatcagtaa gttatttggt aattcctttc
      661 tgtcataaaa atgtcatatt ccttacatat aactgtcacc tgtttgtcct attttgcttc
      721 tcgtagccaa caaacaatgc tttatgaatc ctcccaggag acattatgaa agttatgcgt
      781 accaccgtcg caactgttgt cgccgcgacc ttatcgatga gcgctttctc tgtgtttgca
      841 gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc caaatgggct
      901 gacacttacc agaaagaaac cggtaataaa gttaactacc agggtatcgg ttcttccggt
      961 ggcgtaaaac agattatcgc taataccgtt gattttggtg cctctgacgc gccgctgtct
     1021 gacgaaaaac tggctcagga aggtctgttc cagttcccga ccgtgattgg cggcgtggtg
     1081 ctggcggtta acattccagg gctgaagtct ggcgaactgg tgctggatgg taaaaccctc
     1141 ggcgacatct acctgggcaa aatcaagaag tgggatgatg aagccatcgc caaactgaat
     1201 ccgggtctga aactgccttc acaaaacatt gctgtagtac gccgcgcaga tggctccggg
     1261 acttccttcg tcttcaccag ctacctggcg aaagtgaacg aagagtggaa aaacaacgtt
     1321 ggtactggct ctaccgtaaa atggccgatc ggtctgggcg gtaaaggtaa cgacggtatc
     1381 gccgcgttcg ttcagcgtct gccgggtgca attggttatg ttgaatatgc ttacgcgaag
     1441 cagaacaacc tggcgtacac caaactgatc tccgctgatg gtaaaccggt tagtccgacc
     1501 gaagaaaact tcgctaatgc agcaaaaggt gcagactgga gcaaaacctt cgctcaggat
     1561 ctgaccaacc agaaaggcga agatgcatgg cctattacct ctaccacgtt cattctgatc
     1621 cacaaagatc agaagaaacc agaacaaggc acagaagtgc tgaaattctt cgactgggcg
     1681 tacaaaaccg gggctaaaca ggcgaacgac ctggattacg ccagcctgcc ggatagtgta
     1741 gttgaacagg ttcgcgctgc gtggaagacc aatattaaag acagtagcgg taagccgctg
     1801 tactaataaa actccaggcc gggtacggtg ttttacgccg catccggcat tacaaaatga
     1861 ctttgtaaac gcgtttaact gaagagtaac ttatggctgc aaccaagcct gcttttaacc
     1921 caccgggtaa aaagggcgac ataattttca gcgtgctggt aaaactggcg gcgctgattg
     1981 tgctattgat gttgggtggc attattgtct ctctgatcat ctcctcctgg ccgagcattc
     2041 agaaatttgg tctggctttc ctatggacca aagagtggga tgcaccgaac gatatctacg
     2101 gggcgctggt gccgatctac ggtacgttgg tgacttcgtt tatcgcgctg ctgatcgccg
     2161 tcccggtgag tttcggtatc gccctgttcc tgactgagct tgcgcctggc tggctgaaac
     2221 gcccgctggg tatcgccatt gagctgctgg cagccattcc aagtatcgtt tacggcatgt
     2281 ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt tcaggagccg gtcggcaata
     2341 tcatgtcgaa tatcccgatt gttggcgcgc tgttctctgg ccccgcattt ggtatcggta
     2401 tcctcgcggc aggcgtgatc ctcgccatca tgattattcc gtacattgcg gcggtaatgc
     2461 gtgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc ggcctacggt attggctgca
     2521 ccacctggga agttatctgg cgtatcgttc ttccgttcac caaaaatggt gttatcggcg
     2581 gcatcatgct ggggctgggc cgcgcgctcg gtgaaaccat ggcggtgacc tttatcatcg
     2641 gtaacaccta ccagctcgac agcgcctcgc tgtatatgcc gggcaacagt atcacctctg
     2701 cgctggcgaa cgaatttgcg gaagcggaat ccggtctgca cgttgccgca ctgatggaac
     2761 tgggcctgat cctgtttgtg attaccttca tcgtcctcgc cgcatcgaag tttatgatta
     2821 tgcgcctggc taagaatgag ggggcacgct aatggctatg gttgaaatgc aaaccactgc
     2881 ggcgctggct gaatctcgcc gcaaaatgca ggcgcgtcgc cgcctcaaaa accgtattgc
     2941 gctgacgctc tcgatggcga cgatggcctt cggcctgttc tggctgatct ggattttaat
     3001 gtccaccatc actcgcggta tcgacggtat gtcgctggcg ctgttcactg aaatgacgcc
     3061 gccgcccaat acggaaggtg gtggtctggc gaacgccctg gcgggtagcg ggctgttaat
     3121 tttgtgggcc acggtattcg gtacgccgct gggcattatg gcggggattt atctggcgga
     3181 atatggtcgt aaatcctggc tggcagaagt gattcgcttc attaacgaca ttctgctctc
     3241 cgcgccgtcg attgtggttg gtctgtttgt ctacaccatc gtggtggcac agatggagca
     3301 cttctccggc tgggcgggcg taattgcgct ggcgctgttg caggtgccga ttgttatccg
     3361 caccaccgag aacatgctga aactggtgcc ggacagcctg cgtgaagcgg cttatgcgct
     3421 gggtacaccg aagtggaaga tgatctccgc gattacgctg aaagcgtcgg tgtccgggat
     3481 catgaccggt atcttgctgg cgattgcccg tattgctggt gaaaccgcgc cgctgctgtt
     3541 taccgcgctc tccaaccagt tctggagcac ggacatgatg cagccgatcg ccaacctgcc
     3601 ggtgacgatc tttaagtttg cgatgagtcc gtttgcggaa tggcagcaac tggcctgggc
     3661 cggggtattg atcattaccc tgtgcgtact gctgctgaac attctggcgc gcgttgtttt
     3721 tgcgaagaat aaacacggtt aacactttgc cggatgcggc gtgaacgcct gatccggcct
     3781 acggtaagcc tgattagcga agtgcatcag gcacgatgag gaaaagattg caatgagtat
     3841 ggttgaaact gccccgagta aaattcaggt tcgtaatttg aacttctact acggcaaatt
     3901 ccatgccctg aaaaacatca acctggatat cgctaaaaac caggtaacgg cgtttatcgg
     3961 gccgtccggc tgcggtaaat cgacgctgct gcgtaccttc aacaaaatgt ttgaactgta
     4021 cccggagcag cgtgcggaag gtgaaattct gcttgatggc gacaacatcc tgaccaactc
     4081 tcaggatatc gccctgctgc gtgcgaaagt gggcatggtg ttccagaaac cgacgccgtt
     4141 tccgatgtcc atctacgaca acatcgcttt tggcgttcgt ctgtttgaga agctctcccg
     4201 tgccgacatg gacgagcgcg tgcagtgggc attgaccaaa gccgcattgt ggaacgaaac
     4261 caaagataaa ttgcaccaga gcggttactc tctctctggt ggtcagcaac agcgtctgtg
     4321 tattgcgcgt ggtatcgcca ttcgcccgga agtgctgctg ctcgacgaac cgtgttcggc
     4381 gctcgaccct atctctaccg ggcgtattga agagctgatc accgaactga agcaggatta
     4441 caccgtggtg atcgtcaccc acaacatgca gcaggctgcg cgttgttccg accacacggc
     4501 gtttatgtac ctgggcgaat tgattgagtt cagcaacacg gacgatctgt tcaccaagcc
     4561 agcgaagaaa caaacagaag actacatcac cggtcgttac ggttgattca ggagtgcgtt
     4621 atggacagtc tcaatcttaa taaacatatt tccggccagt tcaacgccga actggaaagt
     4681 atccgcacgc aggtgatgac catgggcggc atggtggagc agcagctttc tgatgcaatc
     4741 accgcgatgc ataaccagga cagcgatctg gcgaagcgcg tgatcgaagg cgacaagaac
     4801 gtcaacatga tggaagtggc gatcgatgaa gcctgcgtgc gcattatcgc caaacgtcag
     4861 ccgacggcga gcgacctgcg actggttatg gtgatcagta aaaccattgc cgagctggag
     4921 cgtattggcg acgtggcgga caaaatctgc cgtactgcgc tggagaaatt ctcccagcag
     4981 catcagccgt tgctggtaag tctggagtcg ctgggccgtc ataccatcca gatgctgcac
     5041 gacgtgctgg acgcgttcgc gcggatggac attgacgaag cggtacgtat ttatcgtgaa
     5101 gataaaaaag tcgatcagga atacgaaggt attgttcgtc aactgatgac ctacatgatg
     5161 gaagattcgc gtaccattcc gagcgtactt actgcgctgt tctgcgcgcg ttctatcgaa
     5221 cgtattggcg accgctgcca gaatatttgt gagtttatct tctactacgt gaaggggcag
     5281 gatttccgtc atgtaggcgg tgatgagctg gataaactgc tggcgggaaa agatagcgac
     5341 aaataattca ccagacaaat cccaataact taattattgg gatttgttat a
//
LOCUS       DAZFRJ010000245         5355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000245 DAZFRJ010000000
VERSION     DAZFRJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.247"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..357
                     /locus_tag="ACHKUN_004338"
     CDS             <1..357
                     /locus_tag="ACHKUN_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662471.1"
                     /translation="MKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGF
                     TAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(326..2062)
                     /gene="aceK"
                     /locus_tag="ACHKUN_004339"
     CDS             complement(326..2062)
                     /gene="aceK"
                     /locus_tag="ACHKUN_004339"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO6662472.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2343..3647)
                     /gene="aceA"
                     /locus_tag="ACHKUN_004340"
     CDS             complement(2343..3647)
                     /gene="aceA"
                     /locus_tag="ACHKUN_004340"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO6662473.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3677..5278)
                     /gene="aceB"
                     /locus_tag="ACHKUN_004341"
     CDS             complement(3677..5278)
                     /gene="aceB"
                     /locus_tag="ACHKUN_004341"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO6662474.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1246 a         1441 c         1368 g         1300 t
ORIGIN      
        1 atgaagcccc tcctcctggc aaataattct aatgaatatc ccggtttgtt ttcagcgata
       61 cagcataaac aacaaaatgt tgtagagacg gtttatcttg ctttatctaa ccatgcacgc
      121 ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc cccacaaaaa
      181 tactctgcct ttgagttggc ttttgaattg ggtcaccggg ttattgctga attaatcctt
      241 aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg atacattgcg
      301 gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt acgctaaatc
      361 tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg cggttttgta
      421 gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac agcggaccaa
      481 tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg cccggcgaga
      541 cgctgtacca cggttcgctg gcaagttcgt cttccggata gcgcggcggc gggatgtcgc
      601 ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc acacgcccgt
      661 gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta gcagcggcaa
      721 gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga ccttccactt
      781 gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg cgaattacaa
      841 tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaatg
      901 ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc
      961 ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca
     1021 tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca
     1081 gcgtaaatac caacatcacc attccacgaa tacccggcgc ttcaatgaac tgctcattac
     1141 agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc
     1201 cgatagccat atacaattca gcagtggttt tacccggcag aatttcccgt agccactcga
     1261 ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag ccaaaaacaa
     1321 tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg tcgtccgtct
     1381 ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg cccaccagcc
     1441 aagcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt tccgcgaggt
     1501 tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca cggcttttat
     1561 tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga gattcccagc
     1621 cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg
     1681 gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac
     1741 agtacacgga gttaaaaaag ctctccgcaa tctcgaagtg cgggtaatcc ggcaacagcc
     1801 gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg ccgttagtaa
     1861 tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt
     1921 tcatcgcctg ctggacagca tgccagtcgg cctgttcgaa acgctgctgc gccccggaag
     1981 tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca
     2041 gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt gccggatgcg
     2101 gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa
     2161 gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct tatccggcct
     2221 acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag gcatcgtgca
     2281 ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac ggttgttgtt
     2341 gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga cgtgccgccc
     2401 tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg gtgagatacg
     2461 aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc aacgtagtgc
     2521 ttcataccct cgccctgggc ataggcgttt gccaggtcaa acatgttgaa ccacatgctg
     2581 tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag ctgctgctgg
     2641 aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg cgagcagtta
     2701 taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg acgcgccagt
     2761 tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc atacgccagg
     2821 ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc actggtacgc
     2881 tcgccagtaa taaattcact gtcatacggg tcgcaatcgg aggtaatcag atccgccgca
     2941 tcggcatcgg tacgagcaac cagcagggtt ggaacgcccg tcacgtcagc tgcaagacgc
     3001 gccgcgacca gtttctgaat agcttcctga gttggcacca aaactttgcc gcccatgtga
     3061 ccgcatttct tcactgacgc cagctgatct tcgaagtgaa ctgccgctgc accggcttca
     3121 atcatcgctt tcatcagttc aaaggcattc aggacaccgc caaaaccggc ctccgcatcg
     3181 gcaacgatcg gcaggaagta atcgacatag cgcggatcgc ccggctcaat gcctgcggac
     3241 cattggatct gatcggcacg acggaaggtg ttgttgatcc gctccaccac agctggcacc
     3301 gagtttgccg gatagagcga ctgatccgga tacatgctgg ccgccaggtt agcgtccgcc
     3361 gctacctgcc atcccgacag atagactgct tcaatacccg ctttcgcctg ttgcagcgcc
     3421 tgaccgccag tcagtgcacc gaggctgttg atgtagcctt ttttcgactc gccatgcagc
     3481 agacgccaca ttttcgccgc gcccagttgc gccagggtgc attcaggatt gactgaaccg
     3541 cgtaatttca ccacatcttc cgcgctgtat gggcgagtaa tgccttccca acgcggttga
     3601 gtccactctt tctgtaattc ttcaatttgt tgtgtacggg ttttcatgtg cagatgctcc
     3661 atagttatgt ggtggtttac gctaacaggc ggtagcctgg cagggtcagg aaatcaatta
     3721 actcatcgga agtggtgatc tgttccatca agcgtgcggc atcgtcaaaa cgcccctggg
     3781 agaaacgttc ttcgcccagt tcgctggcaa tgactttcat ctcttcaccc agcatctggc
     3841 ggaacaaggc tttggtcacc ggtttgccat tgctcaacgt tttttgatga tggatccact
     3901 gccagattga ggtacgggaa atttcagccg tcgccgcatc ttccatcagg ccataaatcg
     3961 gcacgcagcc gttgccggag atccatgctt cgatgtactg cactgccacg cgaatgttgg
     4021 cgcgcatacc ttcttcggtg cgttcaccat cacaaggtgc cagcagctga tcggcagtaa
     4081 tcggcgcgtc ttgttcgcgc atcacttcaa gctgattttt acgggagccg agaatgtcgt
     4141 tgaataccgc cattgccgta tccgcaaggc ctgggtgagc aatccatgtg ccatcgtgac
     4201 cgttattggc ttccagcgct ttatccgctt ttactttgtt gagcacctgg ttattgcgct
     4261 cttcatcttt gctcggaata aacgctgcca tgccgcccat cgcaaaagca ccgcgtttat
     4321 ggcaggtttt aatcaacagg cgtgagtaag cattcaggaa tggtttatcc atcgtcactg
     4381 cctgtctgtc tggcaggacg cgatcgggat agtttttcaa cgttttgata tagctgaaga
     4441 tgtaatccca acgaccgcag ttcagaccaa caatatggtc acgcagcgcg tgaaggattt
     4501 catccatctg gaacacggcg ggcagcgttt caatcagcaa cgtcgccttg atggtgccgc
     4561 gcggcagatt aaagcgatct tctgcatagc tgaagacttc gctccaccag gccgcttcct
     4621 gccaggactg ggttttcggc agatagaaat agggaccgct gccctttgcc aacagtgcct
     4681 gatagttgtg gaagaaatag agcgcaaaat caaacaggct accggggatt gcctcaccac
     4741 gccaggtgac atgtttttcc ggcaagtgca gaccgcgtac ccgacaaatc aaaaccgctg
     4801 gattgggctt gagctgataa attttgcctg cttcattggt atagctgatg gtgccgttaa
     4861 ccgcatcgcg caggttaatt tgcccgtcga tcactttgtt ccagtctggt gccagtgaat
     4921 cttcgaaatc ggccataaag actttcacat tggcgttcag tgcgttgatc accatcttgc
     4981 gctcaaccgg gccagttatc tcgactcgac gatcttgtaa gtccgcggga ataccacgaa
     5041 ttttccagtc cgtatcgcga atggaagctg tttccgaaat aaaatcaggc aaagttccgt
     5101 tatcaatatc ttgctgctgc tgaatgcgcg ctgccagaag tttattgcgt tgtggcgtaa
     5161 aatgcgtcac cagctcagtc agaaattcta ccgcttcggc agtaagaatt tgcttctcct
     5221 gctcgccata cggccttatg aaagccagtt catcggttgt tgttgcctgt tcagtcatcg
     5281 tgcagctcct cgtcatggac ccgaaaactt ccccactgtg aacgaaggat cgttgtgcgc
     5341 ttttcgttca gcaca
//
LOCUS       DAZFRJ010000246         5322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000246 DAZFRJ010000000
VERSION     DAZFRJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.248"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHKUN_004342"
     CDS             complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHKUN_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO6662475.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHKUN_004343"
     CDS             complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHKUN_004343"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO6662476.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHKUN_004344"
     CDS             complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHKUN_004344"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO6662477.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHKUN_004345"
     CDS             complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHKUN_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO6662478.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2344..2739)
                     /locus_tag="ACHKUN_004346"
     CDS             complement(2344..2739)
                     /locus_tag="ACHKUN_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6662479.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHKUN_004347"
     CDS             complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHKUN_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6662480.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3654..4247
                     /locus_tag="ACHKUN_004348"
     CDS             3654..4247
                     /locus_tag="ACHKUN_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6662481.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            4392..4844
                     /gene="tabA"
                     /locus_tag="ACHKUN_004349"
     CDS             4392..4844
                     /gene="tabA"
                     /locus_tag="ACHKUN_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO6662482.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4967..>5322
                     /locus_tag="ACHKUN_004350"
     CDS             4967..>5322
                     /locus_tag="ACHKUN_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662483.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVI"
BASE COUNT         1344 a         1251 c         1330 g         1397 t
ORIGIN      
        1 ttttgcgttt gtcatcagat ttaatccggg catgatagcc cggatttcca tcaagattag
       61 cgacgaacag cgatcgcttc gatctcaatt tttacgtctt tcggcagacg ggcaacttca
      121 acgcaagaac gtgccgggaa ggtggcattg tgttcggtga agaaggcttc gtaggtagca
      181 ttaacggttg cgaagtcgtt cagatctttc acaaacacgg tggttttaac gatgtcgccc
      241 actttcaggc ctgcggcttc gacgatcgct tttacgttat ccagcgactg acgtgcctgt
      301 gcagcgacgt ctgccggtac ttcgcccgtt ttcggattta ccgggatctg gccggaggtg
      361 atgatcatat tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt
      421 tccgtcgcga tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc
      481 attataggga gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg
      541 gaaaactctt tttcacagta tttgcatttg agcgcgatgt cattggcgcg ttttcgcacg
      601 gcaaagctgg aagaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc
      661 acattgtcga tgcgctccgg cagactgggg cgcgatttac ccaccacttc atagttgtcg
      721 atacggttaa ccgtggcttg cggcgcatac aatgccagtt gatctacctg atcttcactc
      781 aaaaaggtat tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaggttc
      841 agaccaatgg tgatgcgctg atccgtttcg gtcagcttga acagactcaa cagcttaaaa
      901 ccgatctggg cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat
      961 ttattatcgt gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt
     1021 gccagtaacg cctggcgagc gaaaatcccg ttgcctgcct gctggaagta ccaggcgtgt
     1081 ggcgttttat caacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc
     1141 atattggctt tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg
     1201 gcgtactcgg acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact
     1261 tccgccatca cttcttcaat agagctgtgc agactccatg cgatcccttt ttcatcgagc
     1321 atatccagaa tgtattgcgg cattgccagc gcgtccggcg cgatgaagta aaaacggttg
     1381 ccatcgaact tcgctaacgc ctgagtcagg gagtgaacgg tgcggccata tttcaggtca
     1441 ccaaccattg cgacgtggag attgtccaga cgcccctggg tttcctgaat agtgaataag
     1501 tccagcaagg tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca
     1561 ttgccggaaa actcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc
     1621 gcatcgacgt aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ctttttaccc
     1681 agtgatgtat tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatagat
     1741 gtttcgaaag agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg
     1801 tgcttcaaca gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga
     1861 ttaaggtcat cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta
     1921 gccatctttt atctcctgac gcctgggcaa aaaaaagccc ctcgattgag gggctgggaa
     1981 tgggtgatca acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca
     2041 aaatgtcgaa cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata
     2101 gccagaagcg gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat
     2161 taaaagtcaa aatgtatgca tttttatttg ctttgattct ggaggagaac tgtcgttcgg
     2221 tatcaataat tgcagacatt tcctgctttt ttctttttca ctttcacgca atcagatatg
     2281 cattttattc cattcatccg acttataggg cggagtgtga aaacgaacgg ctaacactat
     2341 tgcttacagc tcagggatgc gcgctatcac tttaatttca aaatcaaagc ctgccagcca
     2401 ggtaacgccc accgccgtcc agtttggata aggaggggcg ctaaatattt catttttgac
     2461 cgtcatgatg tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat
     2521 atcgtcaaaa gtgcatcccg cagcagccag ggtcgcatgc aaattatcaa atgccagtct
     2581 gacttgttgc tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga
     2641 aacaaacagc aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga
     2701 atgtcggcca gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac
     2761 gacatgaaca gaagatttct ctttaccggg agccgctttt agcggacgac gtgagtaaac
     2821 aaaacccaga catcatggat aatggctggg cttaattgag cgtagtcggt tatgcgccaa
     2881 acgcgccatc aatggtatgc atcgcgccgg taacaaaact ggcttctggc cctgctaacc
     2941 atgcgaccat accagcgacc tcttccggtt gcccatgtct tttgatagcc atcaaactat
     3001 gcaacatatc gcgcattggc ccgttggcgg gattagcgtc ggtatcaatt ggccctggct
     3061 ggacgacgtt aatggtgatc ccacgcggtc caaaatcacg ggccagcccg cgcgccatgc
     3121 cttgcagggc agatttgctg gcggcataag cagccatgcc tgcaacaggc atacgatcgc
     3181 cattcacgga gccgatgatt aagatgcgcc cgccttcggg catctgccgg gcggcttcaa
     3241 cagaggcatg ataaggagca tgaatattga ttttgaaaag gcgatcaata tcgtcggcat
     3301 ttaattccag ggcatcgcca aagacgccaa tacctgcatt taccaccagg atatccaatg
     3361 cgccgctctt acgaacgaca tcaatgacag cgtctctgtc agcactatct gtaaatactg
     3421 ctgtcgctcc agtctcttgt gccagatgtt cagcggcatc tttcgacccc gcataggtga
     3481 atcgtacatt ggccccatca gtgacgaaac gacgtacgat agcggcaccg attccacgac
     3541 tgccaccgag gatgagaact gtcttacctg taaaagcgcc cataaggact ccttgattta
     3601 ttatgtaaca tgcattacaa aactgtttta actttctgtc aacaagtttt gtaatggtca
     3661 ctaaaaaaca atctcgcgtt ccaggtcgtc ccagacgttt cgctcctgag caggcagtct
     3721 ctgcggcaaa agtgcttttt caccaaaaag gtttcgatgc tgtcagtgtt gctgaagtta
     3781 ctgattatct tggtattaac cccccgagcc tctacgcagc ttttggcagt aaagttgggt
     3841 tatttagccg tgtactcaat gaatatgtcg gtacggaagc tattccgctt gccgatattc
     3901 ttcgtgatga tcgtccggta ggcgagtgcc tggttgaggt attaaaagaa gcggcgcgca
     3961 gatatagcca aaacggcggc tgcgctggct gtatggttct tgaaggtatt catagtcatg
     4021 atccacaagc gcgtgatatt gccgttcaat attatcacgc cgcagaaacg accatttatg
     4081 actatatcgc caggcggcat ccacaaagcg cacaatgtgt gactgatttt atgagtaccg
     4141 tgatgtcagg gctttctgcg aaggcacggg aggggcactc catcgaacaa ctctgtgcaa
     4201 cagctgcact ggcgggggaa gcgataaaaa ctattctcaa ggagtgatgc tggccttgac
     4261 ccgaaaggcg ggaacgcgcc tgccgataag ttgtgataag acaataattc acgcgttaag
     4321 gctagcggaa ttgatcatct tttcgtataa cggtggaaat gaaacattgt tttaattaag
     4381 gagtggaaaa gatgatcatc ggaaatattc ataatcttca gccgtggcta ccccaggagt
     4441 tacgccaggc gattgagcat atcaaagcgc acgttacggc agaaacgcca aagggtaagc
     4501 acgatatcga aggcaatcgt ctgttttatc ttatctcgga agatatgacc gagccgtacg
     4561 aagctcgccg tgcggagtat catgcccgct atctcgacat tcagattgtg ttaaaaggtc
     4621 aggaaggcat gaccttcagc acgcaacctg caggcacgcc ggataccgac tggttagctg
     4681 ataaagacat cgcatttttg ccggaaggcg ttgatgagaa aacagttatc cttaatgaag
     4741 gtgattttgt tgtgttttat ccgggggaag tgcataaacc gctgtgcgca gtgggcgcgc
     4801 cagcccaggt tcgcaaagca gtagtgaaga tgctgatggc gtgatgactt ttcgccgtaa
     4861 ataactccag gtttacggcg agtttgtgaa aagagcgttt tttgatattt ttttgtgagt
     4921 agaatttgta atgcttagac gttcttatta actcaaggag tccgtcatgg caaaaatatc
     4981 agatttgaat tgttatcaac acatggcatc agccgacaat gttaaaccaa aaaatgaagt
     5041 cttaaatacc tggtgtgtgg gaatgaatga ttttgctcgt aatgccgagg ggcaggatag
     5101 cagaaggaat attctatctc ctggagcatt tttggagttt ttggcaaaaa tatttaccct
     5161 gggttatgtg gattttagca aacgctccaa cgaagcgggt agaaatatga tggctcatat
     5221 tgagtcctca tcttatatca aaaataatga tggcagtgaa ataatgaatt ttgttatgaa
     5281 taatcctgaa ggtgagcgtg cggattcatc taaggtgatt at
//
LOCUS       DAZFRJ010000247         5260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000247 DAZFRJ010000000
VERSION     DAZFRJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.249"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..245
                     /locus_tag="ACHKUN_004351"
     CDS             27..245
                     /locus_tag="ACHKUN_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6662484.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            223..1293
                     /locus_tag="ACHKUN_004352"
     CDS             223..1293
                     /locus_tag="ACHKUN_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6662485.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTH
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            1428..2711
                     /gene="ybhC"
                     /locus_tag="ACHKUN_004353"
     CDS             1428..2711
                     /gene="ybhC"
                     /locus_tag="ACHKUN_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO6662486.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(2945..5206)
                     /gene="ybhJ"
                     /locus_tag="ACHKUN_004354"
     CDS             complement(2945..5206)
                     /gene="ybhJ"
                     /locus_tag="ACHKUN_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO6662487.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
BASE COUNT         1367 a         1326 c         1308 g         1259 t
ORIGIN      
        1 ttgcgtgtaa ttgcggagac tttgcgatgt acttgacact tcaggagtgg aatgcacgcc
       61 agcgacgccc aagaagcctt gaaacagttc gtcgatgggt gcgcgaatgc aggatattcc
      121 ctcctccggt taaggatgga agagagtatc tgttccacga atcagcggta aaggttgact
      181 taaatcgacc agtaacaggt agccttttga agaggatcag aaatgggaag aaggcgaagt
      241 catgagcgcc gggatttacc ccctaatctt tatataagaa acaatggata ttactgctac
      301 agggacccaa ggacgggtaa agagtttgga ttaggccgag acaggaggat agcaatcact
      361 gaagcaatac aggccaacat tgagttattt tcaggacaca aacacaagcc tctgacagcg
      421 agaatcaaca gtgataattc tgttacgtta cattcatggc ttgatcgcta cgaaaaaatc
      481 ctcgccagca gaggaatcaa gcagaagact ctcataaatt acatgagcaa aattaaagca
      541 ataaggaggg ggctgcctga tgctccactt gaagacatca ccacaaaaga aattgcggca
      601 atgctcagtg gatacataga cgagggcaag gcggcgtcag ccaagttaat cagatcaaca
      661 ctgagcgatg cattccgaga ggcaatagct gaaggccata taacaacaaa cccggtcgct
      721 gccactcgcg cagcaaaatc agaggtaagg agatcaagac ttacggctga cgaatacctg
      781 aaaatttatc aagcagcaga atcatcacca tgttggctaa gacttgcaat ggagctggct
      841 gttgttaccg ggcagcgagt tggtgattta tgcgaaatga agtggtctga tatcgtagat
      901 ggatatcttt atgtcgagca aagcaaaaca ggcgtaaaaa ttgccatccc tacaacattg
      961 catgttgatg ctctcgggat atcaatgaag gaaacacttg ataaatgcaa agagattctt
     1021 ggcggagaaa ccataattgc atctactcat cgtgaaccgc tttcatccgg cacagtatca
     1081 aggtatttta tgcgcgcacg aaaagcatca ggtctttcct tcgaagggga tccgccgacc
     1141 tttcacgagt tgcgcagttt gtctgcaaga ctctatgaga agcagataag cgataagttt
     1201 gctcaacatc ttctcgggca taagtcggac accatggcat cacagtatcg tgatgacaga
     1261 ggcagggagt gggacaaaat tgaaatcaaa taatgatttt attttgactg atagtgacct
     1321 gttcgttgca acaaattgat aagcaatgct tttttataat gccaacttag tataaaaaag
     1381 caggcttcaa cggattcatt tttctatttc atagcccgga gcaacctgtg aacacatttt
     1441 cagtttcccg tctggcgctg gcattggctt ttggcgtgac gctgaccgcc tgtagctcaa
     1501 ccccgcccga tcaacgtcct tctgatcaaa ccgcgcctgg tacctcttct cgcccgattc
     1561 tgtcggcaaa agaagcgcag aatttcgatg ctcaacacta ttttgcatcc ctgacaccag
     1621 gtgcagcagc gtggaatcct tccccgatta ccctgcctgc gcaacctgac tttgttgtcg
     1681 gcccggcggg cactcaaggt gtaacgcata ccacgattca ggcggcggta gatgcggcaa
     1741 ttatcaagcg taccaacaag cgccagtata ttgccgtgat gcctggtgag tatcagggaa
     1801 cggtatatgt ccctgccgct ccgggtggaa ttactctgta cggtacaggt gaaaaaccga
     1861 ttgatgtgaa gattgggctt tcccttgatg gtggcatgag ccctgccgac tggcgtcacg
     1921 acgtcaaccc gcgcggcaaa tatatgccag gtaaaccagc gtggtatatg tacgatagct
     1981 gccagagcaa acgcagcgac agtatcggtg ttctctgctc tgcggtcttc tggtcacaaa
     2041 acaatggcct gcaactgcaa aatctgacca tcgaaaacac gctgggcgat agcgtagatg
     2101 caggtaacca tccggcggtg gcactgcgta ctgatggtga ccaggtacag attaacaacg
     2161 ttaacattct cggtcgtcag aacaccttct ttgtcaccaa cagcggtgtg cagaaccgtc
     2221 tggaaacgaa tcgtcagccg cgtacgctgg tgaccaacag ctacattgaa ggggatgtgg
     2281 atatcgtttc tggtcgcggc gcagtggtgt tcgataacac cgaattccgc gtggtgaact
     2341 cacgtactca gcaagaagcg tatgtgtttg caccggctac gctgtccaac atttactacg
     2401 gtttcctcgc cgtaaacagc cgtttcaatg ctttcggtga tggtgtggcg caactgggcc
     2461 gctcgctgga tgttgatgcc aataccaacg gtcaggtggt gatccgtgat agcgccatca
     2521 acgaaggttt taacacggct aaaccgtggg ccgatgcggt gatctctaat cgtccgtttg
     2581 cgggtaatac cggcagcgta gatgataacg acgaaataca gcgcaatctg aatgacacta
     2641 actacaaccg catgtgggaa tacaataacc gcggcgtggg tagtaaagtg gttgcagagg
     2701 cgaagaagta agagcaatta actatttgcc ggatgcggcg taaacgcctt atccggccta
     2761 cggttcgatg cgatttgtag gtcggataag atgcgcaagc atcgcatccg acaataagtg
     2821 ccggatgctg cgaaaatgcc ttatctggcc tacagattcg atgcgattcg taggtcggat
     2881 aagatgcgca agcatcgcat ccgacaataa gtgccgaatg cgacctacat tcacatggcg
     2941 ctttttacat ctgacggttt ttattgaagt taatcaaact acccgccttg ataatctcgc
     3001 gctcttcagc agtcagactt tccatataga gcgtaatttc cgttaccggc gcatcttcat
     3061 ggatcacata acctttaaac gtcgtacccg gattatccag cgccgcttta atgccaggga
     3121 tgtaaatgta atcccccact tcaaaggttg gcacttccgc catctgcagc ggtaacatcc
     3181 cccagttgat gacgttagaa cggtagcgtt tagtcgcgta ctcttcggca atattcgcca
     3241 gaccgccaat cacacgctgg cagctcgccg cctgttcacg cgcagaacca tcgcctggtt
     3301 tcaccgcata taccatgctg ccaatttcag tttgcagcgg atcaatatgc tcctgaccag
     3361 caatctgctt aatgcgcgca aacacctctg tcagctcgct gacattcccc gccagacgct
     3421 gattttccag ctcagcagtc gctttacttc tgccaacata accgggatcg cggcgtgaca
     3481 gggtaaactc cgccagacca atcggatttg aacgataaga agaggtttca ccggaaggaa
     3541 tcagttcgtc ggtggtggtc acttcgtcga ggatcttcga gcacactttc aggacgatat
     3601 tgtcagtcag cgcacccaat tccggccagt ctttaatgtt cggtccgtaa atcagcggtt
     3661 gctgagttgc ccctttcaca aagccctgat aaacacggtt tttatacggc gttacatcga
     3721 aggcgtactc cggcacgttg tcccagcaat caagttcgct ggcagaggtt aaatagccac
     3781 cgtttgccgc agtcgcagcg atagaacgag cgtccatcaa cgccaccgct gacatctgcc
     3841 cattagctgg cttagagcct tcgcggttcg ggaagttacg cgtggtgtgg cgaatactca
     3901 aaccgttgtt gattggcgta tcgcccgcgc caaagcatgg gccgcagaac gcggttctga
     3961 tgattgcgcc tgcgccaatc aaatctgcta ccacaccttt tttggcgaga tccataaaca
     4021 ccggctgtga tgacgggtaa actgccagcg agaaggtgtc attgccacag gattgaccgc
     4081 gcagtgcatt cgccgccgcg atgacgtttt cgtagttacc gccagaacag cccgcgataa
     4141 tcccctgctg cactttcagg cgaccatttt ccactttatc cagcagcgag agtttggctt
     4201 taccgtgcgc cacgcgttcg gactcaattt caatctcacg cagaatgtcg gtcaggttct
     4261 ggttcagtgt gtcgatttca tacacgttgc tcgggtggaa cggcagcgca atcattggtt
     4321 tgatggcgct taaatcaacg ctgatgcagc catcgtagta cgccatcggt tgagggttaa
     4381 gctggcagta atcctggccg cgaccgtgca gcgccagcca gttatgcact tcttcatcag
     4441 tttgccagac ggagcttaaa caggtggttt ccgtggtcat cacgtcaacg ctgttacgga
     4501 aatcggtaga gagcgcgcta acgcccggtc caacgaactc catgactttg tttttgacgt
     4561 aaccgttttt gaacaccgcg ccaatgatag ccagcgccac atcctgtggc cccacatacg
     4621 gcgcaggttt tccggtcaga tgcaccgcaa ccacgcccgg atagtcgata tcccaggtgt
     4681 cattaagcag ctgttttacc aactcaccgc cgccctcacc gactgccatt gtccctaatg
     4741 caccgtaacg ggtgtggctg tctgacccga ggatcatttt gccgccgcct gccatcatct
     4801 cacgcatata ttgatggatg accgcaatat gcggaggcac aaaaataccg ccataacgct
     4861 gggccgccga taaaccaaaa acatggtcat caccgttaat agtgccgccg acggcgcaga
     4921 gtgagttatg gcagttggtc agcacatacg gcagcgggaa acgttccata ccggacgctt
     4981 tagcggtctg tacaatacca acaaaggtaa tatcgtgaga ggctaatgaa tcaaacttaa
     5041 ttttaagttt atccatattt ccggacgtat tatgcgaaga gagaatagac caggcaatag
     5101 tgcctttttt ggcttcttct tttttaattt cgccggtgaa atgttcttcg gcaattattt
     5161 cgttattact ggcgagaaac acgccttttt cagataactt gatcatcaaa tgctccagat
     5221 atctgcgcac cgtgaagtgc gcagaaagat aataaatgga
//
LOCUS       DAZFRJ010000248         5208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000248 DAZFRJ010000000
VERSION     DAZFRJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.250"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..430
                     /gene="hpaR"
                     /locus_tag="ACHKUN_004355"
     CDS             2..430
                     /gene="hpaR"
                     /locus_tag="ACHKUN_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO6662488.1"
                     /translation="MALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESPSMDFHD
                     LAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQIEE
                     AYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            549..2213
                     /gene="tsr"
                     /locus_tag="ACHKUN_004356"
     CDS             549..2213
                     /gene="tsr"
                     /locus_tag="ACHKUN_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO6662489.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(2256..3527)
                     /locus_tag="ACHKUN_004357"
     CDS             complement(2256..3527)
                     /locus_tag="ACHKUN_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6662490.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(3524..3997)
                     /locus_tag="ACHKUN_004358"
     CDS             complement(3524..3997)
                     /locus_tag="ACHKUN_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6662491.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            complement(4061..5032)
                     /locus_tag="ACHKUN_004359"
     CDS             complement(4061..5032)
                     /locus_tag="ACHKUN_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO6662492.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1362 a         1252 c         1341 g         1253 t
ORIGIN      
        1 cattgcgttg ctccaggcgc gagaagcggc gatgagttat tttcgcccaa tcgttaaacg
       61 gcataatttg actgagcaac agtggcgcat cgtgcgtatt ctggcggaaa gcccatcaat
      121 ggattttcac gatctggcgt atcgcgcctg cattttgcgc ccaagcctga ccgggatcct
      181 cacgcggatg gaacgcgacg gtttagtgtt gcgattaaag ccgatcaatg atcagcggaa
      241 gctctatatc tcgctgacca aagaggggca ggcgctgtat aaccgcgctc agacacagat
      301 tgaagaagcc tatcggcaga ttgaagctca gtttactgcc gagaaaatgc aacagttaac
      361 ccatctgtta gaggaattta ttgctctggg taattcccga caagaagaca tcccgggcga
      421 taatgaataa ctttttagta tctgataacc agcgcctggt ttttccatgg atggcgggtt
      481 acaccttttc ataaagtttt tgctttccag gccgaaaatc ttgcatcggt ccacaggaaa
      541 gagaaaccat gttaaaacgt atcaaaattg tgaccagctt actgctggtt ttggccgttt
      601 ttggcctttt acaactgaca tcaggcggtc tgttctttaa tgccttaaag aatgacaaag
      661 aaaatttcac tgttttacaa accattcgcc agcagcaatc cacgctgaat ggcagctggg
      721 tcgcgttgtt gcagacgcgt aacaccctca accgcgcggg tatccgctac atgatggatc
      781 agaataatat tggtagcggt tcaaccgttg ctgagctgat gcagagtgcc agtatttcgc
      841 tgaaacaggc ggaaaaaaac tgggcggatt acgaagcgtt gccgcgtgac ccgcgtcaga
      901 gcaccgccgc agcggcagag atcaaacgta attacgatat ttatcacaat gcgctggcgg
      961 agctgatcca actattaggt gcaggcaaaa tcaacgagtt ctttgatcag ccgacccagg
     1021 gatatcagga cggtttcgag aagcagtatg tggcttacat ggagcaaaac gatcggctct
     1081 atgatatcgc cgtcagcgat aacaatgcct cctacagcca ggcgatgtgg attctggtgg
     1141 gcgtgatgat cgtcgtactg gcggtcatct tcgccgtctg gttcggtatt aaagcctcgc
     1201 tggtagcgcc aatgaatcgc ctgattgaca gcattcgtca tattgcaggc ggcgatctgg
     1261 tgaaaccgat tgaggtggat ggctctaatg agatggggca actggcagag agtttgcgcc
     1321 atatgcaggg agagctgatg cgtaccgtcg gtgatgtgcg caacggggcc aatgccatct
     1381 atagcggtgc cagcgaaatc gctaccggca ataacgatct ctcttcgcgc accgagcaac
     1441 aggccgcttc gctggaagag acggcagcca gcatggagca actgaccgca acggtgaaac
     1501 agaacgccga gaatgcgcgc caggccagcc atctggcatt aagtgcttct gaaacggcgc
     1561 aacgcggcgg caaagtggta gataacgtgg tgcagactat gcgcgatatc tccaccagtt
     1621 cgcagaaaat cgccgatatt atcagcgtaa ttgacggcat tgccttccag accaatattc
     1681 tggctttgaa cgcggcggtt gaggctgcgc gtgcgggtga gcaaggacgc ggttttgcgg
     1741 tggtcgcggg agaagtgcgt aatctggccc agcgtagcgc tcaggcggct cgtgaaatta
     1801 aaagcctgat tgaagactcg gtgggaaaag tggatgttgg ctctacgctg gtcgaaagcg
     1861 ccggggaaac aatggcggag attgtcagtg ctgtgacccg cgtgacggac attatgggcg
     1921 aaattgcttc tgcttctgat gagcagagcc gtggtatcga tcaggttggc ttagcggttg
     1981 ctgagatgga ccgggtaact caacagaacg ccgcgctggt ggaagaatct gccgccgccg
     2041 ccgccgcgct ggaagagcag gccagtcgcc tgaccgaagc tgtggcagtg ttccggattc
     2101 agcaacagca gcaacagcag cgtgaaacat cggctgtggt aaaaaccgtg acgccagctg
     2161 cgccgcgtaa aatggccgtg gcagatagcg gggagaactg ggaaacgttt taacgcaatg
     2221 acgccggatg agtatttcat ccggcgtttt tattattaaa ccaggaacag tgaaatcgcc
     2281 gggatatatg tcaccagcgt tagcattaat agtagagccg ccagtagagg cagggattgt
     2341 ttaaagaaat ttcccaccga aacttgcgtg atgttacata ccagcagcat cacggtacct
     2401 accggtggcg tgaatgcgcc aatcgacagg ttgaagatca tgatgatgcc aaaatgcacc
     2461 ggatcgatgc caaagtgttc gaccaccggt tttaatagtg gtaccaggat cagcaaaatg
     2521 gcgtttcctt caagaaacat ccccagcgtc agcagcaata tgttgataat taacaggaac
     2581 atatatttat tttcggtcag cgaagtcaca aaattcgcca ctgcgacacc agcatgttcc
     2641 attgagaaaa tccatcccaa cgcggcgctg gccatgatta ccagcatcac cgatgcggtt
     2701 gcgcgtgccg tttcgcccaa tgctttgacg acatgagaaa tccgcatttc acgatagata
     2761 aaaaagccaa tcgccagcac gattagcacc gctgctgcac ctgcttctgt tggggtgaaa
     2821 atgttagcgc gaataccacc caccacagca aaaatgagga acagcgcagg ccaggcctgt
     2881 cccagcgaga acaccacctc tttgcccgta ggccagcttt cacgcgaggg tttagcgtta
     2941 gttttgcggg cgaccaggta gacggtaaac atcagaacga ctgcgcatag caaaccaggt
     3001 accagcccag cgataaacat ttttccgata gagacgtcgg caaccagacc gtaaataatc
     3061 agggctattc ccggcggtat gatcggggtg actaacgagc caccagcggt aatggcggcg
     3121 gcaaacgcgc ggttgtagcc tttacgctcc atctccggca ccatcatgcg cgacagcatc
     3181 gccgcgtcag caaggttaga ggcgctgacg ccgcccatca tggtgctgac gagaatgttt
     3241 gccacgccaa gtccgccgcg cattttcccc accagaacgt tggcaatgtg caggatgcgt
     3301 tcggcaatgc cggtgtagct cattagtgtg ccgagcaaga taaaaaacgg aattgccagc
     3361 aatgatgggc tttgcgttgg cgcgatcagt ctttgcacgg caatttcaat cggtacgctg
     3421 gagaagaata aaaagtaagc gatgatgcca ataaacatcg ccagatagac ccgaatgttg
     3481 agaaacaaac agacgatcat tagcggaata atcaataacc aactcattgc attttgtctc
     3541 cgctatcaga ttgttttata tcgtgtatta accagtagag ggtataaata gccgttgcca
     3601 gtgcaccgac agggaccgcc agatcgatcc agaaccagga aaccttcaga agttgtgtca
     3661 ctttaaactg cgccatttcg gctagtcggt agcccagcca ggccatatac agcagcagtg
     3721 cgatggagag aacggcgacc agtactgcaa taacacgcct gagcttgcct ggaagcatct
     3781 ccatcaggaa gggaatggtc aggtgcgccc tgtcacgttc cgccaccact ccacctaaca
     3841 tcaccaccca aatcatcagc attcccgaca tttcttctgt ccattgaagc ggttggccga
     3901 tgaaataacg cataaatacc gctccaattg tcatcacaac cagaacgaat agcgcaaagg
     3961 cagcactaaa ttctgccact ttgccaaaaa gacgcatgaa acctcctgtt agtgcgtgtc
     4021 ctgcctaaga gagatgaggc aggacgctga gcattaatgc ttattgcagt tgctgttgaa
     4081 tggtttcgta cagacctggt gtccattccg ggaattgaga gtaaacttcg cgtgctttct
     4141 tctggaatgg tccagtatcg ggatagatca cttcgacgcc cgctgcctgc atggttttca
     4201 acatttcggc atcacgttca agagtgagtt tctggctata gacacccgct tcatagccgg
     4261 tctggtggat catctccagt tgttctggtg aaagggtgct aaagaaggct tcacctccta
     4321 tccatactga ggtatttgtc aggtagttaa ccattgtcag atatcttgcc tgctcataca
     4381 gtttttgccc ttgcagcact gaaattgggt tttctacgcc gtcaataacg ccctgggtta
     4441 atgcgggata aacttcgcct aatggcatgg gagttggtgt tgcccccatc gcctgaatgg
     4501 cttttatttg catgacgtta ttaggaacac gaatttttaa gcctgcgaga tcgtcaacag
     4561 tgcgaatggg ttttttcgaa ataatctgtc gagtgccata gagataatta ttcattacca
     4621 catgaattcc ctttttcttc agactttcat ttttttgttt aaaccagtcg ctttcatata
     4681 ttttgaacag tttttgcgga tcgtcagtca gatacggtcc aaacaaaata cccaaatcgg
     4741 gttcataatc ggcaagaaag gccacatcac tcaaggttat gacattcatg cccatcatgg
     4801 cttgctcggt gacatcctgt tttgagccca attgggaact tggatagagc gcaagtgtta
     4861 tttcgccatt acttttttta tttaataggt ctgcccagta acgcatgacc acatccaggg
     4921 gttctccagg gttattttca taggcaactt ttatcgaaat aggttttgcc tggattattg
     4981 acggtaaaaa aagggtactg acagtaatta gcatcgctat tattttgctc atctttactt
     5041 actccatgct aaggaaataa aactccaaca ttgttgtcgg gagtgaaacc ttatgaaaaa
     5101 gcagtcatta ctgaaaagta gtcagcgaat agcggaatgg ctgcaaatgt gtcataaatt
     5161 cgtgcaccgg gtaatacttt gctgctttta atcggttttt aatgcatt
//
LOCUS       DAZFRJ010000249         5184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000249 DAZFRJ010000000
VERSION     DAZFRJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.251"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..227)
                     /locus_tag="ACHKUN_004360"
     CDS             complement(<1..227)
                     /locus_tag="ACHKUN_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO6662493.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANT"
     gene            complement(368..1009)
                     /locus_tag="ACHKUN_004361"
     CDS             complement(368..1009)
                     /locus_tag="ACHKUN_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO6662494.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            1709..2581
                     /gene="repA"
                     /locus_tag="ACHKUN_004362"
     CDS             1709..2581
                     /gene="repA"
                     /locus_tag="ACHKUN_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO6662495.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     SSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            3485..3769
                     /locus_tag="ACHKUN_004363"
     CDS             3485..3769
                     /locus_tag="ACHKUN_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO6662496.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDLMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            3769..4044
                     /locus_tag="ACHKUN_004364"
     CDS             3769..4044
                     /locus_tag="ACHKUN_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6662497.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(4567..5169)
                     /locus_tag="ACHKUN_004365"
     CDS             complement(4567..5169)
                     /locus_tag="ACHKUN_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6662498.1"
                     /translation="MGKHQEERKIPVIVVKKRRTFSEPSLSEKTDIIAPVFTEQTTES
                     VSAGINSSAVEKHIPEASARKKKKKKHRFPRPSHWTREYTHECVEKIKILFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPEAELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSESEAQSAGRRLATLEQKWLRMQAQQENISGQ"
BASE COUNT         1308 a         1276 c         1352 g         1248 t
ORIGIN      
        1 gtgtttgcta ccggtatgtc tgaattggtg gccagcagtt ttcgcagggt agtgaactct
       61 tcagccattc ctctgaactc gcgcatctga cgggcagtgt caagccagtt cagcaggtaa
      121 cgccggcgac gccgttcgtt gtttatgttg tccggggcgg tggagagtgc ttcgagcata
      181 ttctccagta acagaatgac agggttttcc tgggatagaa tggtcataaa ggcgtcctga
      241 aaaaatgccc gacagccgct ctccgtacac cctcaggtgt caccggtgcc gggcattgtg
      301 gaaacagaag ggaaaaaaat cccggccata aaggccggga aaagtcgtca aattaagaca
      361 gcaattttca tgtcaacgct ccgtcatcag ataccaggtg caggagcagg gcgatcctcg
      421 actccggcag aggctgtgca tcaagccgga ggaggtactg gtaacgcgct tttgtcagcc
      481 agagggtgga ggcagcatcc agctcctccc tggctgccgg attgagcgca ggatccgggt
      541 acactttcat caaagactga acgatatcct ccctgacggt tttgatttca ccggccattt
      601 tcacgaagtc cagaaaggca ctgtgacggc gaaggtgagt gaagctgtgg cgttcggggt
      661 ctgctttcag gaaaaagtaa ccgggaaaca cggcgtgtat atgtcgacgg taaccacaga
      721 ctttatccgt gcggcggacc tttttcagta tcaggggtgt ccagggaagg acgtgttgtt
      781 cggaaagcca ggaaaagaga tgttcgcggt ttttgccggc agggatgtac tgagccagat
      841 accagttgcg ttgatgtaat tcactgatat tcaaaatccg gaacgctccg cgaaaatcga
      901 tccccgcaga ggggcttcga tgttgttgtg aacggcgaat acataaagca tgtgtcggaa
      961 acagcggctg accctcgttc agcccggcct gcagttctga caggcacatg atttacgcac
     1021 tcttgtaagc cttgtggacg gtttcgaggc gggcgaaaac cgacaactcc tgcaaacagg
     1081 attaatggct ggcaaatata ttacataagg atgtatctgt ggcaagagtg aagataagca
     1141 gttaaataga tcgttatatt tgataaagca gcatattatg ctgatttgtg tgcggtgtct
     1201 ggaggattag atcgtttaaa aattttaggc gatctcttct actaaaaatg atgaaaaata
     1261 cagcataaaa gatcgtttgt ggaattgtct atttttttga catagttcaa aaaaacgacg
     1321 ttttttaggg ggttaatgag aggcctggta aatcagagaa atcgtgtcgg cttttcaaag
     1381 cggtggaaaa ggggtatatt gcggatcgtt atgcagtggc ttttgggatc gtcaggatcc
     1441 gggaagtcag aaaacggcag gatgcgccat aaggcattca ggatgtatgg cagaaacgac
     1501 ggcggttggc cggggccgga aggctgaaaa agtttcagaa gaccataaag gaaaaccccc
     1561 actatctttc gtcgaacttt ggcgggctca agaaagatag tgggggcctc acgaatacgg
     1621 gataagtata tatgaaaccg tgtcagagat tcaacccttt gcagtgcatt aacgcacggc
     1681 aatctgttcc cgccataagc gacagcttgt ggcggccctg aaccagacct catttactga
     1741 aagcacatac cgtcaggtaa agaacccgaa tccggtgttc acaccccgtg aagatgccgg
     1801 aacgctgaag ttctgcgaaa aactgatgga aaaggcggtg ggcttcacct cccgttttga
     1861 ttttgccgcg catgtggcgt atgcccgttc gcgcggcctg cgccggcgta tgccgccggt
     1921 gctgcgccgc cgggcgattg atgcgctgct gcaggggctg tgtttccact atgatccgct
     1981 ggccaaccgc gtccagtgct ccatcaccac gctggccatt gagtgcggac tggcgacgga
     2041 gtctgaggcc gggaaactct ccatcacccg tgccacccgt gccctgaagt tcctgtctga
     2101 gctggggctg ataacctacc agacggaata cgacccgaca attggctgta acatcccgac
     2161 cgacatcacg ttcacgccgg cactgttttc ctcactcgat atatcagaag aggcggtggc
     2221 ttccgcacgc cgcagccgcg tggaatggga aaacaagctg cgcaaaaagc aggggctgga
     2281 tgcgctgggt atggatgaac tgatagcgag agcctggcgt tttgtgcgcg agcgtttccg
     2341 tagttatcag gcagagctta agtcccacgg catgaaacgt gcccgtgcgc gccgcgatgc
     2401 aggcaggacc cgtcaggaca ttgtcaccct ggtgaaacgg cagctgacac gtgaaatcgc
     2461 ggaagggcgt ttccgcggaa gcctggaggc cgtgaagcgc gaaatcgacc gtcgcgtgaa
     2521 ggaacgcatg ataatgtcgc ggaacaacaa ctacacacgg ctggccacgg cctcaccctg
     2581 agtttgacct catctgaata atccggcctg cgccggaggt atccgcacgc ctgaagcccg
     2641 caagcgcaca aaaaagcagc cccacacaca aaaaacagcc acgtcatcca ccttcaggtg
     2701 catccggttc cccctgtttt tcacgcaaaa cacgtctcac agacagggaa ttcttcttgt
     2761 ccacatttaa ctgcaaggga tttctccgta aggttacaac cgctcattcc ataaagcgcc
     2821 agccgctggc gttacaggct gcaatgtttc ttttaaaccc cagtactgta tttcctttaa
     2881 actacttaat tacttccatt taaaaagaaa acctattcag tacctgtcct gtggacaagc
     2941 aaatatgcac ctcccacgct aacgcgcggg cccccaacct caccgttctg aaaccacagc
     3001 aaaaagacat caggaataaa aacaccacac aaacgcagca gcgtgccacc cctcataact
     3061 gaaaagcgag gccgcccccg cccgaagggc gggaacaaca tcgcttttaa ttatgaatgt
     3121 tgtaactaag actcattatt gctgtcagtc ttctggctgg atgttctcag tacacgctcg
     3181 taagcggccc tgacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaccctcgt
     3241 cgggaccact ccgaccgcgc acaaaaactc tctcatggct gaaagcgggt atggcttagc
     3301 agggctgggg atgggtaagg tgaaatctat caatccgtac cggcttacgc cgggcttcgg
     3361 cggttttaca cctgtgctac atgtaaccac cgtatgccgc cttacatgcg ctgacgcgca
     3421 ttatttgcct tgttccgtct gaaagtaatc actatgatta aatatgatta acaactaatc
     3481 ggatatgcaa atgaaaaaca atactgcaca agcaacaaaa gtaattaccg cgcatgtgcc
     3541 attacctatg gctgataaag tcgacctgat ggcagcaaga ctggaacgct cccggggctg
     3601 ggttatcaaa caggcgcttt ctgcatggct tgcccaggag gaggagcgta atcgcctgac
     3661 gctggaagcc ctggatgatg tgacatccgg acaggttatc gaccatcagg ctgtacaggc
     3721 ctgggcggac agcctcagta ctgacaatcc gttaccggtg ccacgctgat ggaactgaag
     3781 tggaccagta aggcgctttc tgatttggcg cggttatatg attttctggt gctggccagt
     3841 aaacctgcgg ccgccagaac ggtgcaatcc ctgacacagg caccggtcat tctgttaact
     3901 catccacgta tgggagaaca gttgtttcag tttgaaccca gggaggtcag acggattttt
     3961 gctggcgagt acgaaatccg ttacgaactt aatggccaga ctatttatgt attgcgcctg
     4021 tggcacacac gagaaaacag gtagattgtg tgtggggaca acttaacagg gggccgcttg
     4081 gaattcggaa aataaaccac gttgagacta ttttttagtc aaataacttt caggaatcgc
     4141 cagaatggcg ggacaacagc ggttttggtg tccgaaatcg tacaattctg tatatctacg
     4201 caaggaaatg tacggaagta agccaaatgg ttatcggacg gtcggaacat ttcgtacagc
     4261 ctttacgttc agtgagttag ctggcttgga tataaccact cgttcaactc aattctctta
     4321 gcgtgggaaa atttccgaat tccaagcgga cccctaagta cgtaattatt gcctgatatg
     4381 tatataatca ggagaaccac tgaaatccag aatgccagtt ccaatacagt atatacaggc
     4441 ttcaggcgtc tgcatgcctg ctcatgttta ttcaggataa atacgatttt atctgcatct
     4501 gatttactca taatcattct cttcccattt ttctgatacg taatgtccag ctgttatcag
     4561 gagtgtttat tgcccagaaa tattctcctg ctgtgcctgc atcctgagcc acttttgttc
     4621 taatgtcgcc agtctgcgac cggcactctg tgcttcagat tctgagacct gccctttggg
     4681 gtgaccatcc agaccataac gtgggacgcc ggcgacagct gtccgttgca gatatacacg
     4741 gcgtgaggtg atgcaggaca ctgcacagag ccattcgtcc atggtcagtt ctgcctccgg
     4801 gtgttctgcc agaaatgccg aaatatcatt gtttatgcca attttcaggg gaataaatcc
     4861 accgccttca gcacgaagat gaggaaacag aatctttatt ttttccacgc actcgtgagt
     4921 atactctcgg gtccagtggg agggacgtgg gaaacggtgt tttttctttt tcttcttcct
     4981 ggctgacgct tcagggatat gtttttcgac ggcagatgaa tttattccgg cggatactga
     5041 ttctgtcgtc tgttcagtaa atacaggtgc gataatatct gttttttcgg ataaggaagg
     5101 ttccgaaaaa gttcgacgtt ttttcaccac aatgactggt attttccttt cttcctgatg
     5161 ttttcccata aatacctcgg ctgt
//
LOCUS       DAZFRJ010000250         5183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000250 DAZFRJ010000000
VERSION     DAZFRJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.252"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..811)
                     /gene="dsbG"
                     /locus_tag="ACHKUN_004366"
     CDS             complement(65..811)
                     /gene="dsbG"
                     /locus_tag="ACHKUN_004366"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO6662499.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1183..1746
                     /gene="ahpC"
                     /locus_tag="ACHKUN_004367"
     CDS             1183..1746
                     /gene="ahpC"
                     /locus_tag="ACHKUN_004367"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO6662500.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1917..3482
                     /gene="ahpF"
                     /locus_tag="ACHKUN_004368"
     CDS             1917..3482
                     /gene="ahpF"
                     /locus_tag="ACHKUN_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO6662501.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3603..4031)
                     /gene="uspG"
                     /locus_tag="ACHKUN_004369"
     CDS             complement(3603..4031)
                     /gene="uspG"
                     /locus_tag="ACHKUN_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO6662502.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4252..>5183
                     /locus_tag="ACHKUN_004370"
     CDS             4252..>5183
                     /locus_tag="ACHKUN_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO6662503.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYA"
BASE COUNT         1316 a         1258 c         1310 g         1299 t
ORIGIN      
        1 aattcatgct tttttaggat ggttgaaaag cagggatgta tttcaaccat ccatgaatta
       61 tcttttattt attccccata atgatattaa gcgttttctg atcgggcaac cccacggcct
      121 gttgcagcgt attttcctta ctcatgtaat agatcgctgg tgtgacattt gcccccagat
      181 cgtccatgag tttctcgttg gcgcttaaca ctttcatttg ttctgtactt acgtttgcag
      241 gcacgtttag cttaagcttg ccaccagagg attcatattc ttgccaggtt tttgcgggat
      301 ctttggaggc aagaattgcc gctgctgtcg ccgggctttc tggcttgata accccaacca
      361 acaatgttct taattgcact ttgccagaat ctacccacgg gcgcgcctgc tgccagaact
      421 gtttacaata tgggcagaac ggatcggcga acacgtagac aatgaccggc gcatcttttt
      481 taccgtcgag gagccagtgg gattgttcca tccgttgcca catttcgcgt ccggctggtg
      541 cgtaaatttc tttttcgata agtgtgttac tcaggttttc acctttctcg ttgtacatgt
      601 aaccagagat agcgtgctta ccatctggag tcaggtagat ggtgacgccc atatcctgat
      661 actttccgag ataacctttc attcctccgg gggcatcgaa tgttttgatg attgtaatgc
      721 cctgtttttc aatcgctttt actggagcag gaagttcctc tgcgaaggcg attgcaggaa
      781 gcagagccag taaaagtatc ttttttaaca ttaatttgtc cttttcagtc agtgcaaaag
      841 tcgagtaaaa ggcataacct atcactgtca taggtaagag cttagatcag gtgattgccc
      901 tttgtttatg agggtgttgt aatccatgtc gttgttgcat ttgtaagggc aacacctcag
      961 cctgcaggca ggcactgaag ataccaaagg gtagttcaga ttacacggtc acctggaaag
     1021 ggggccattt tactttttat cgccgctggc ggtgcaaagt tcacaaagtt gtcttacgaa
     1081 ggttgtaagg taaaacttat cgatttgata atggaaacgc attagccgaa tcggcaaaaa
     1141 ttggttacct tacatctcat cgaaaacacg gaggaagtat agatgtcctt gattaacacc
     1201 aaaattaaac cttttaaaaa ccaggcattc aaaaacggcg aattcatcga aatcaccgaa
     1261 aaagataccg aaggccgctg gagcgtcttc ttcttctacc cggctgactt tactttcgta
     1321 tgcccgaccg aactgggtga cgttgctgac cactacgaag aactgcagaa actgggcgta
     1381 gacgtatacg cagtatctac cgatactcac ttcacccaca aagcatggca cagcagctct
     1441 gaaaccatcg ctaaaatcaa atatgcgatg atcggcgacc cgactggcgc cctgacccgt
     1501 aacttcgaca acatgcgtga agatgaaggt ctggctgacc gtgcgacctt cgttgttgac
     1561 ccgcagggta tcatccaggc aatcgaagtt accgctgaag gcattggccg tgacgcgtct
     1621 gacctgctgc gtaaaatcaa agcagcacag tacgtagctt ctcacccagg tgaagtttgc
     1681 ccggctaaat ggaaagaagg tgaagcaact ctggctccgt ctctggacct ggttggtaaa
     1741 atctaaattt ccttcgtctt tcgcgctacc aactccggcg cgaaatactt cggaaattca
     1801 cctaattctt cgggtgctgc ggcacccgat tttcttcccc gcaccatgat gcaagctgca
     1861 tccaggtagc cgcagaggcc gcttgcatga tgatgtttaa agcccaggag ataaacatgc
     1921 tcgacacaaa tatgaaaact caactcaagg cttaccttga gaaattgacc aagcctgttg
     1981 agttaattgc cacgctggat gacagcgcta aatcggcaga aatcaaggaa ctgttggctg
     2041 aaatcgcaga actgtcagac aaagtcacct ttaaagaaga taacagcttg ccggtgcgta
     2101 agccgtcttt cctgatcacc aacccaggtt ccaaccaggg gccacgtttt gcaggctccc
     2161 cgctgggcca cgagttcacc tcgctggtac tggcgttgct atggaccggt ggtcatccgt
     2221 cgaaagaagc gcagtctctg ctggagcaga ttcgccatat tgacggtgat tttgaattcg
     2281 aaacctatta ctcgctctct tgccataact gcccggacgt ggtgcaggcg ctgaacctga
     2341 tgagcgtact gaacccgcgc atcaagcaca ctgcaattga cggcggcacc ttccagaacg
     2401 aaatcaccga tcgcaacgtg atgggcgttc cggcagtgtt cgtaaacggg aaagagtttg
     2461 gtcagggccg catgacgttg actgaaatcg ttgccaaaat tgatacgggc gcggaaaaac
     2521 gtgcggcaga agagctgaac aagcgtgatg cttatgacgt attaatcgtt ggttccggcc
     2581 cggcgggtgc agctgcagca atttactccg cacgtaaagg catccgtacc ggtctgatgg
     2641 gcgaacgttt tggtggtcag atcctcgata ccgttgatat cgaaaactac atttctgtac
     2701 cgaagactga agggcagaag ctggcaggcg cactgaaagt tcacgttgat gaatacgacg
     2761 ttgatgtgat cgacagccag agcgccagca aactgatccc agcagcagtt gaaggtggtc
     2821 tgcatcagat tgaaacagct tctggcgcgg tactgaaagc acgcagcatt atcgtggcga
     2881 ccggtgcaaa atggcgcaac atgaacgttc ctggcgaaga tcagtatcgc accaaaggcg
     2941 tgacctactg cccgcactgc gacggcccgc tgtttaaagg caaacgcgta gcggttatcg
     3001 gcggcggtaa ctccggcgtg gaagcggcaa ttgacctggc gggtatcgtt gagcacgtaa
     3061 cgctgctcga atttgcgcca gaaatgaaag ccgaccaggt tctgcaggac aaactgcgca
     3121 gcctgaaaaa cgtcgacatt attctgaatg cgcaaaccac ggaagtgaaa ggcgacggca
     3181 gcaaagtcgt tggtctggaa tatcgagatc gtgtcagcgg cgatattcac aacatcgaac
     3241 tggccggtat tttcgtccag attggtctgc tgccgaacac caactggctg gaaggcgcag
     3301 tcgaacgtaa ccgcatgggt gagattatca ttgatgcgaa atgcgaaacc aacgtcaaag
     3361 gcgtgttcgc agcgggtgac tgtacgacgg ttccgtacaa gcagattatc atcgccacag
     3421 gcgaaggtgc caaagcctct ctgagtgctt ttgactacct gattcgcacc aaaactgcat
     3481 aagaagaagt aagattcacc tgcaattgct tagccgccgg ggtcaaacct ggcggctttt
     3541 ttatggcatt aaaaagccct gccgggatgg ctccgggcag ggcgggatat ttattctggc
     3601 aattaacgca caaccagcac cggcagattg gcatggcgga tcacgctcga ggcgttagaa
     3661 cctaacagat gggtcgaaat cgatgggttg cgagaaccaa taactacaac atcagccccc
     3721 agttcttctg ccaactcatt gacttcatcc cgcacgctac caaaacggac atgttgttta
     3781 atgcgggaag gatcgatggt gaagtggctg accatcgttt gcagacgttc ttctgcttca
     3841 tgttgcagat gctcttcaaa acgacgcaca tcagcggcaa aacggtgcag gctcaggctg
     3901 gctgacccgg gtagtacgtg aagtagatga ataactccgt catcctgggc gaggaattca
     3961 gcgtggcgaa cagctttgtc gctcaattcc atttcaaata catcaactgg cataatgatt
     4021 gtcttataca taaccctttc tccctgttaa tcatgaacaa atcattcgcc atgattataa
     4081 tatttatccc tgatatttgt ctggttcttt ttccttacga acggtttctg tgattaatat
     4141 attctcactg aacaccagga attctcccaa aacctgcggt accgcccgtt ttcccgctgt
     4201 gatagctacc cttaaagact gactcttttt tgaactgtct ctggaggttg catgaaagca
     4261 ttgacttatc acggcccaca tcacgttcag gtagaaaatg ttcccgatcc gggcattgaa
     4321 caggcagatg atattattct gcgtattacg gcaacggcga tctgtggctc tgacctccat
     4381 ctttatcgag gcaaaatacc ccaggttaaa catggcgata tttttggtca tgaatttatg
     4441 ggggaagtcg ttgaaaccgg aaaggacgta aaaaatttgc aaaaaggcga ccgggtggta
     4501 attccgtttg tcattgcttg tggcgactgt tttttctgtc gaatgcagca atatgccgcc
     4561 tgcgaaaata ccaatgcggg taaaggcgct gcgctcaata aaaaacagat accagctccc
     4621 gcggcattgt ttagttatag tcacctgtat ggtggcgttc ctggtggaca ggcggaatat
     4681 gtccgcgtcc ctaaagggaa tgtggggccg tttaaagtac cgcctttgct ttcagatgat
     4741 aaagcccttt tcctttctga tattctgcca acggcatggc aggcagcaaa aaatgcgcag
     4801 atccaacaag gttcaagcgt tgcagtctat ggtgccggtc cagtgggatt gttgacaatc
     4861 gcctgtgcac ggttgctcgg tgcggaacag atttttgttg ttgatcatca tccctaccgc
     4921 ttgcgtttcg ccgctgaccg ctacggcgcg atcccgatta atttcgatga agatagcgat
     4981 ccggcacagt caattattga acaaacggca ggtcaccggg gcgtggatgc agtaatagac
     5041 gccgtcggtt ttgaagcgaa aggcagcacc acggaaacgg tgctgactaa cctgaaactg
     5101 gagggcagca gcggtaaagc gttgcgccag tgtattgcgg cggtcaggcg tggcggcatt
     5161 gttagcgtac cgggcgtcta cgc
//
LOCUS       DAZFRJ010000251         5123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000251 DAZFRJ010000000
VERSION     DAZFRJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.253"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..834
                     /locus_tag="ACHKUN_004371"
     CDS             <1..834
                     /locus_tag="ACHKUN_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6662504.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDLTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHKUN_004372"
     CDS             876..1271
                     /locus_tag="ACHKUN_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6662505.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1283..1657
                     /locus_tag="ACHKUN_004373"
     CDS             1283..1657
                     /locus_tag="ACHKUN_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6662506.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1648..2226
                     /locus_tag="ACHKUN_004374"
     CDS             1648..2226
                     /locus_tag="ACHKUN_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662507.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHKUN_004375"
     CDS             2223..2618
                     /locus_tag="ACHKUN_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6662508.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHKUN_004376"
     CDS             2626..3366
                     /locus_tag="ACHKUN_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662509.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATDMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHKUN_004377"
     CDS             3382..3804
                     /locus_tag="ACHKUN_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6662510.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHKUN_004378"
     CDS             3786..4220
                     /locus_tag="ACHKUN_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6662511.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..>5123
                     /locus_tag="ACHKUN_004379"
     CDS             4213..>5123
                     /locus_tag="ACHKUN_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO6662512.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT         1134 a         1312 c         1641 g         1036 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacctgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggttgtat
     1081 cagcgcagcc ggataccgtg actgatacgg ctgatctggt cacggttgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt gtgctgccgg
     1201 gaacggcgtt ccgtgtctct gccggtgtgg cagctgaaat gacagagcgc ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc
     1321 ctgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgcggcgaca cgctgaccat cggtgaggaa aacttctgga tagaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac cgcctgccgt
     1621 taaccgtcgc cgctggaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc cattaaccgc
     1741 gttgcttcat ccgcgatatc gcagtcggtg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc
     1921 ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     2401 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gagttgatca
     2521 ccagtatggt ggccagtggc tatgactacc gacgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgcg
     2821 gactggactg cgaccgggca ggggcagaaa tcagccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cgacatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     4501 ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta
     4801 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggactgcgcg ggc
//
LOCUS       DAZFRJ010000252         5006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000252 DAZFRJ010000000
VERSION     DAZFRJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.254"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(158..233)
                     /locus_tag="ACHKUN_004380"
     tRNA            complement(158..233)
                     /locus_tag="ACHKUN_004380"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ile,seq:cat)
     gene            complement(283..1332)
                     /locus_tag="ACHKUN_004381"
     CDS             complement(283..1332)
                     /locus_tag="ACHKUN_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6662513.1"
                     /translation="MINTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWEGDDDYLKWLDQCLAQFWRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSELNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(1483..1680)
                     /locus_tag="ACHKUN_004382"
     CDS             complement(1483..1680)
                     /locus_tag="ACHKUN_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6662514.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(1912..2454)
                     /locus_tag="ACHKUN_004383"
     CDS             complement(1912..2454)
                     /locus_tag="ACHKUN_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO6662515.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(2463..2828)
                     /locus_tag="ACHKUN_004384"
     CDS             complement(2463..2828)
                     /locus_tag="ACHKUN_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6662516.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            complement(2829..3884)
                     /locus_tag="ACHKUN_004385"
     CDS             complement(2829..3884)
                     /locus_tag="ACHKUN_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6662517.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(3886..4158)
                     /locus_tag="ACHKUN_004386"
     CDS             complement(3886..4158)
                     /locus_tag="ACHKUN_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662518.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            4105..4284
                     /locus_tag="ACHKUN_004387"
     CDS             4105..4284
                     /locus_tag="ACHKUN_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662519.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            complement(4326..4481)
                     /gene="hokD"
                     /locus_tag="ACHKUN_004388"
     CDS             complement(4326..4481)
                     /gene="hokD"
                     /locus_tag="ACHKUN_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6662520.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT         1189 a         1347 c         1142 g         1328 t
ORIGIN      
        1 ttcgaaccag tgaccgattg cttagaaggc aattgctctg tccggctgag ctaacaacgc
       61 ataatgcaga taatggattg ccatcgggga cccgggcccc acacagccag tttcgaaagc
      121 tgacgctctc tgccgatgag ctaatggcgg tatgtgatgg tggcccttgc tggatttgaa
      181 ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg gccggtagca
      241 gaataataat ggtgcgtaat taattctgca atcccatccg tttcaaacga ttaaatcctg
      301 aacttccctg actgtctgtt caaaacgtcc tgtctccagc tcaacaccaa tcgcacaacg
      361 ccccagtgcc atcgccgctt ttaccgttga acctgaaccc ataaaaaaat ctgcaaccag
      421 gtctcccgga cgactgctcg cgttgattat ctgctgcagc atttctgccg gtttttcgca
      481 cggatgtttc cctggatagt actgcaccgg tttatgcgtc cagacatcgg tgtacggaac
      541 ctgcgccgtc acaccgaaat accgccgcaa atttttatat tcactcagca gttccgtata
      601 ctgccggttc agctcactgt atgtgctgac cagctggtgg tgtggctttt ccagttcccc
      661 gcgctgatgt ttttctgccg caacacgcgc aaacaacgcc tgcaatttgt tgtaattacc
      721 ctcgttcggt aactgccact gactggtacc aaaccagtgc gaagccatgt ttttctttcc
      781 ggtggcttcc gctatctgtt ttgacgttat tcccagtgat ttacgcgcat cacgaaagta
      841 agaaatcagc ggggccatga cgtgctgttt tagctcgcgc ccctgtgcca cataaccatc
      901 atctttcggg cgatacggtc cctgataatg ttctgcaaac agaatgcgct ctgttgccgg
      961 aaaatacgcc cgcagacttt ccttattgca cccgttccag cgtccggacg gcttcgccca
     1021 gataatatgg ttcagcacat taaagcgctc acgcatcatg atttcggtgt cagatgccag
     1081 gcgatgacca aagaacaggt aaagacttcc ggcaggcttc agtacccgcc agaactgcgc
     1141 cagacactgg tccagccatt tcaggtaatc atcgtcgccc tcccactggt tatcccagcc
     1201 ctcgggcttc actttaaagt atggcgggtc tgtgactatc agatcgacag agttttccgg
     1261 taaggtctgg ataaattcca ggcaatcagc gttgattaac tcacaactgg atatttttac
     1321 agtattaatc atagatcaat aagcacttct ctgataggct cataccgctt ttgcgcaaag
     1381 cagatgggcc tgaggtttgc ttgtgacccc aacgcatgag cagatggctg gcaggtgccg
     1441 ctaacaccca ccagccgccc attaccacaa attaaaaagc cttcactgcg gaaggcgtct
     1501 gtaacaaccg aactgataat ctgccagacc cgccataaca agctgagtca gtattaactg
     1561 gcagcgttcg cgtgaaaggt aagtattctg cgcaatttcc ccgacggtcg ccggttcggt
     1621 gacgcttaat tcattaaaca ccactctggc ggtttcggtc atatcctgct gttttagcat
     1681 gcttttttcc cttttccggt taacgtgaca taccaatacc tcttgtcgaa aaagccagca
     1741 agctgaaaga ccagtattca caactaccag cgcgtttaat gttctgtgcc gtttttcaga
     1801 cataaaaaaa cccgcataaa gcgggttctt tcaggtgtcc atgtctgcta ttcgcctcgc
     1861 ggtatagctt tgcgaagcgt agctggattg aaacagttta tgcgtaaaaa atcaagacat
     1921 tttttgagca aacgattctc gcatagggat gtatagcgca tattcagcaa cagccaacca
     1981 attagcaatt cgcttttcgc atgtactaaa acaccattcc gggtgcacct cattcaacaa
     2041 ttcagccatt ttgcgtttac tcatcccccg cccttcgtac ctttgccgca ggatatcaat
     2101 caatccagga taacgtgcaa gcgctttact tatcacccca tcaatgcgta acgcctctgc
     2161 atcagtacag tgagacaacc agctcttctg tctgccagcg atcatctctc gcaagaatgc
     2221 ttccagctct ggtttatcaa tccctgactc cctgattcta cgcagggctt cattgattgc
     2281 ggtttttgtc agttttttgg atgccagcaa ctgattgaac atatttcctg gtttgccacc
     2341 acctatatac gaccaacgcc cccacatccg taatttcccc tggatccaga cggcttccag
     2401 cgtttttaga cgtaaatgct cgccgctttt gcctgtaatt tccgggtata tcatatttac
     2461 gatcactcac tctcaatttt gtaaatcttc acgcccagcc gtcccccagg aacgcgctga
     2521 ccgcgcacaa tattgatttc atcaaactgc tcgtcgtcta tgagaagtcc ggcatgcgtc
     2581 agcgcatcca gtggtgcttt caggatattg tccaggtcac gacgacgttt atccggtggc
     2641 tctgcaataa tctttatcgc cagccttccg gacaggttta atttcagccg ctgctggcga
     2701 acaataagcg ccacatcacg gcgataacgc tcaccggctt ttgatacaaa atatgtgctg
     2761 ccacgacgtc gccagtaagt gttcaccgtt ggcgggtaag gcaaaacaaa ttctatgcgt
     2821 tcagtcattc atgctttcca cttcaggaca cccgaatttc tcgcgtgcat taaaaaacga
     2881 atcagcaaca acagctggct gccgtgtttt tcttcaaaat cttttacccc ggcgtgtagt
     2941 tcgctatggc atttacggca cagcggaata acaaacaaat catcagcctt tgttcccatc
     3001 cctcccagtc catgaccaat gatgtgatgc ggatcatctg cctgattacc acacgtcatg
     3061 catttctgcg tttttaccca acgcgtgtat acaggcatct cttcccgtcg tggtttctgg
     3121 cgctggagat actgagccgg tgactccgga tcaacggcaa tgcttaccac cgtcttttcc
     3181 tgtggcgggt tttgctggtg ggcgtgaggc agtagcgcaa tattttttgt gcgctgcttc
     3241 agcatgctgg tggcggtctg ctctcccggc acgatgtcgc tctcgcggta tactgagcga
     3301 attttttccg cgcgtaatcc cagtgaacga cgtaacaccg tctccggtag tgcgtccgct
     3361 acgttattta tggttgccca ccaggataat tcagccagcg ataattcccg ctcctgcgtg
     3421 ccattcattg cgtggcggat gacatcaatc atccatgctg acaggttttg gtgagcaagc
     3481 tgcccgagtg attcggaagt ctggttacgc agctggttgt cgcagtgcca gcacaacacc
     3541 atcgcgccgg taccgtaacg atgtatgacg gtttcactgt gatggtagtc accatgaggc
     3601 cactggcagg atttaatgtg gcgtaacagc cagtcagaca atgcaccagc accaccagca
     3661 gcacgaatca cccgctcatc gctgaaaaat ggcagtaatg atttatcctc cgccagcggc
     3721 tggcgaacag caggaacgac tccggacggc agaccgcgca tgcttttcgg ttccggctcc
     3781 accagaactc gagggttatg aaatacctgc atggattcac ggcccggttt tagcaccacc
     3841 agcccaagtt ccggtaccgg aacaggtcga agtaataccc gcacgttacc tccagatgcg
     3901 ttgctggaat gtgcgggacg gacgcggtgg gcgttcggaa taagggagcc tgacatagat
     3961 tatccagtga cgataatcga ggctgagggc tttcttaatc tcgtatccgc gtctgcggta
     4021 gttatgaatt agccattcgg cctgttcttc agtacatggt gggtgttggt accagtcggt
     4081 tttaaatgcg tgtgaacgcc gcccatgccg gatggcaagg tcggtatcag aattgtgaaa
     4141 tttggttttg tgcaccatct gttttctctg ctggcgcagc aggtgtcagg tgttcaggct
     4201 gacgtgcgaa ttgtaaacca gaatgccagg aaaaaacaaa acccgccgaa gcgggttaag
     4261 tgcgggtgcg ttgaggatgc ctgactcatc agaggtggcg agggatttct ccctcgcctg
     4321 gtctcttact cctcaggttc gtaagctgtg aagacagcga cctccgtctg gccggttcgg
     4381 attcgtacct cgcagaggtc tttcctcgtt accagtgccg tcactatgac ggttaaacag
     4441 atgacgatca gggcgattaa catcgccttt tgctgcttca tagcctgctt ctccttgcct
     4501 ttcggcacgt aagaggctaa cctacatgtg caaagcatga aattggcctc agattaatgt
     4561 taagcgtctt gccggacgcg taatgttaac tggggctttt ctctatctgc cttttggtgt
     4621 tcatgcctga ggcagatagc ctcaagcacc cgcagcaatt ctaactatcc aatagatcaa
     4681 tgccaacttc ttttcctgca acatctctca tcagaagtga cccaaattca tccaatcagc
     4741 acaaaagaaa catttaaagc gtgaatgcta ccgaacgttc atttcaatta catcgttttg
     4801 atttttaaca gttttcacct caacaaatca aatggcatta gcattcatca tggaagaaag
     4861 actgataaaa atgatcattt tcaatgactt atgatcacac cgggtattgc caagtcattc
     4921 cttttacgcc aggataccca caagtgagta gtcgaggaga gtttgccaat ttttgcgata
     4981 taaatttcaa tgtccgaaga accatt
//
LOCUS       DAZFRJ010000253         4978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000253 DAZFRJ010000000
VERSION     DAZFRJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.255"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            175..528
                     /locus_tag="ACHKUN_004389"
                     /pseudo
     CDS             175..528
                     /locus_tag="ACHKUN_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(789..971)
                     /locus_tag="ACHKUN_004390"
     CDS             complement(789..971)
                     /locus_tag="ACHKUN_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662521.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICFLQKVMIIDKTKYK"
     gene            910..3000
                     /gene="iha"
                     /locus_tag="ACHKUN_004391"
     CDS             910..3000
                     /gene="iha"
                     /locus_tag="ACHKUN_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO6662522.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRNVLKRDKWGLAGQLRE
                     LKESNLILNSLLLTPLGESHLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANITGFMTDFSNKIVSY
                     SINDNTNSYINSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3254..3611)
                     /locus_tag="ACHKUN_004392"
                     /pseudo
     CDS             complement(3254..3611)
                     /locus_tag="ACHKUN_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            3831..4595
                     /locus_tag="ACHKUN_004393"
                     /pseudo
     CDS             3831..4595
                     /locus_tag="ACHKUN_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1294 a         1184 c         1269 g         1231 t
ORIGIN      
        1 tagcattcgg cgtataagtt gacagagaag gagtatctca gcaggagctt aaagtgtggt
       61 tttaggcaga atttgattga tcctccattt ggggcatgtg actaatcgat atagcttcct
      121 gaacggaaaa agtgttttcc cttccaatat aaactattcg acaataatat aaatcaatta
      181 cagaataaaa ctctgtataa acatgcaaaa atggtaaagg cgttcgacta tatcctgaat
      241 cactggaatg cgctgaacga gttctgtcgt gacggctggg tggaaataga caacaacatc
      301 ggtgaaaacg cgttacgatc ggtggcggtt ggaagaaaaa attatctctt cttcggctca
      361 gacaaggggg gagaaagcgc ggcgatcatc tacagtctgc tggtcacctg caaactgaac
      421 gaagtggagc cggaggactg gttgcgcgaa gtgatcgtga agctcaatga ctggccgtcg
      481 aaccgtgtgc atgaactgct gccctggaac tgctcaactg taaaataatc cttacgctac
      541 gtacttctcg gggcgcttac gaatttttca cgtgccacag aattttgaac gctttctctt
      601 acaatatttc aatgtttcta tcagtattcg ccggaagaag tcatcgacca aatcatccca
      661 gtcgtctcgc accactgacc attcatggtt gacatgtggt ggaaatccgc ttctacagta
      721 accatttttt attcgcaaaa ccgaacacgc catacggata gctgttaact ggcatgccga
      781 ggcagtcgtt atttatattt agttttgtca ataatcatta ctttttgcaa aaagcaaata
      841 taaattattc tcattattgt ttgtatttgt gtattgtctt gccggttaac atgatcggag
      901 attagtaata tgcgaataac cactctggct tccgtagtca ttccctgtct cggattttca
      961 gccagcagca tagctgctgc agaggatgtg atgattgtct cggcatccgg ctatgagaaa
     1021 aagctgacta acgcagccgc cagtgtttct gtgattagcc aagaggaatt gcagtccagc
     1081 cagtaccacg atctggcgga ggctctgaga tcagtagagg gtgtggatgt tgaaagtggt
     1141 acgggtaaaa ccggtgggct ggaaatcagc atccgaggaa tgccagccag ttacacgctg
     1201 atactgattg atggtgttcg tcagggcgga agcagtgacg tgactcccaa cggtttttct
     1261 gccatgaata ccgggttcat gccccctctg gccgccattg agcgtattga ggttatcagg
     1321 gggccgatgt ccacactgta tggctctgat gcgatgggcg gtgtggtgaa tatcattacc
     1381 agaaagaatg cagacaaatg gctctcttcc gtcaatgcag ggctgaatct gcaggaaagc
     1441 aacaaatggg gtaacagcag ccagtttaat ttctggagca gtggtcccct tgtggatgat
     1501 tctgtcagcc tgcaggtacg cggtagcaca caacagcgtc agggctcatc ggtcacatca
     1561 ctgagcgata cagcagccac gcgtattcct tatcccacgg agtcacagaa ttataatctt
     1621 ggtgcacgtc ttgactggaa ggcgtcggag caggatgtgc tctggtttga tatggatacc
     1681 acccggcagc gttatgataa ccgggatggg caactgggga gtctgacggg gggatatgac
     1741 cggaccctgc gctatgagcg aaacaaaatt tcagctggct atgatcatac tttcaccttc
     1801 ggaacatgga aatcgtatct gaactggaac gagacggaaa ataaaggtcg tgagcttgta
     1861 cgcaatgtac tgaaacgcga caaatggggc cttgccggtc aactgcggga gcttaaggaa
     1921 tctaacctta tcctgaattc attgctgctt acccctctgg gagaatctca tctggttact
     1981 gtggggggcg agtatcagag ttcgtccatg aaagatgggg ttgtccttgc cagcacaggt
     2041 gaaactttcc ggcagaaaag ctggtcggta tttgctgagg acgagtggca tctcacggat
     2101 gcacttgcgc tgactgcggg cagccgctat gaacatcatg agcaattcgg ggggcacttc
     2161 agtccgcgtg catatctggt ctgggatgtg gcagatgcct ggacgctgaa aggcggtgtg
     2221 accacgggat ataaggcacc cagaatgggg cagctccata aagggattag tggtgtgtcc
     2281 gggcagggaa aaacaaatct acttggtaac cccgacctga agccggaaga gagcgtcagt
     2341 tatgaggctg gggtgtatta cgataacccc gccggtctga acgccaatat cacaggtttt
     2401 atgactgact tctctaacaa gattgtctct tattccataa atgataacac caatagctat
     2461 ataaacagcg gaaaggcccg gttgcacggt gtggaatttg ccggcacatt gccgctatgg
     2521 tcagaggatg tcacgctgtc gctgaattac acctggaccc gaagtgaaca acgtgatggt
     2581 gataataaag gtgcaccgct gagttatacc ccagaacaca tggtgaatgc gaaactgaac
     2641 tggcagatca ccgaagaggt ggcatcatgg ctgggtgccc gttatcgcgg gaaaacacca
     2701 cgtttcaccc agaattattc gtcgctgagc gctgtacaga agaaagtata tgatgagaaa
     2761 ggagaatacc tgaaagcctg gacggtggtg gatgcaggtc tgtcgtggaa ggtgacggat
     2821 gccctgacgc tgaatgcggc agtgaataac ctgctcaaca aggattacag tgacgtgagt
     2881 ctgtacagtg ccggtaagag tacgctttat gccggtgatt acttccagac gggatcatca
     2941 acaacgggat atgtgatacc tgagcgaaat tactggatgt cgctgaacta tcagttctga
     3001 taacaacaaa acgctatcac tgacggtagc atacgttgcc actgcaactc ctggcggaac
     3061 ggtggcaatg ttttaggtta agtgcgtttc cggtccgcta atgagatttc gttaccaaca
     3121 actaatatca tcaccggaaa tgcacggatt attttttaac tcatcattta catacttgtc
     3181 cagaaattta tactcggcta gctctgaatt aagggccgcg tccgcatctc cattaccgtc
     3241 ttatatcact ggcgctgaca attgtctcac atatagctgc agtatcggct ccatccccgt
     3301 ggctacgcgc tgaaattcta gtgaaccgct ttcgtggact tgagtgaacc aactgtttag
     3361 ctgtgattta tttggggggc agaatacggt agaactcgag tgccagttgc tgcgctattt
     3421 tctgttccgg ttctgtttca caaatcggga cgcataattt tcttccccct gattattttc
     3481 cagggcacca ccccgcagct tcccagggac agtgaaggaa gtcgggcggc aatagcaaac
     3541 ggcgggatcc aacctttacg ccatttggca acagtatctc tgatgatagt ttcagtgcct
     3601 gtaactcccc gttactttat tcgtaccctt gtaatggggt gttagccagc cccggcatga
     3661 ttaattcccc cagtcgtcca tgattcgggg ggatgtcacc gggtctggtg gggcgctggt
     3721 aaccgctgat aggggcctgg tcagacatca tgtcgggaca gtctgtaacg tggatgccgg
     3781 tacctgctcc ccaaggttat ctggttaacc cgtataaacg gggagacaca atgcccgaat
     3841 ccagcgatta cgaatcaatc ctggtcttta ttggcgttga tgtaggtaaa gacacccatt
     3901 acgccgttgc cgttaatcgt tcaggtaaac gtttgttcga cagagtatta cccaacgacg
     3961 aaaacaaact cagagcgctg atatctgacc tgaaacagca tggacagata ctgctggtcg
     4021 ttgaccagcc tgctaccatc agggcgttac ccgtcgccgt tgcccgctct gaaggcgtcc
     4081 ttgtcggata ccttcctgga ctggcaatgc gacgcatcgc cgatttgcac tccggtgaag
     4141 ccaaaaccga tgcccgtgat gcggccatca ttgccgaagc tgcacgtact ctgcctcatg
     4201 ctcttcgctc tctaaaactg gccgacgaac aaatcgccga actctccatg ctttgcggct
     4261 tcgatgatga tcttgccgca cagacaacac aggccagcaa ccgcatccgc ggcttgttga
     4321 cccagataca tccggctctg gagcgcgttc tcggcccgcg actggatcat cctgcagtac
     4381 ttgatcttct ccagcgatat ccctcacccg aaaaactcgc tttactgggt gagaaaaaac
     4441 tggcaaccca gctctgcaaa ctcgcgcccc gtctgggaaa acgccttgct gcagatatcc
     4501 cccaggcact gacacagcaa accgtcatcg tccccggtac gaatgctgct gtcgttgttc
     4561 tcccacgcct ggcactccag ctcatcacgc tgcgttagcc aagagacgaa gtgactggct
     4621 tcaactataa gtttgttaat accagtgacg ctgatgtcgt tacaggcgat agcacaggac
     4681 gcaagacgat ggtaacggta atagagaggc agatgaacca tccactggaa gattgcggac
     4741 ctctcttaaa attagtacat tacggaccgc cacgctggaa agacagcata tggaaaggct
     4801 ccctgtcaaa cattttgttt aacccggacc ctgaccatct aaccttggca agtggaagat
     4861 tagcgcctct ttgaaaaagg atcaaacaca acacagcaga aaacgattat agtgaatatt
     4921 gaaactatgt gcttatcaaa aaatctgtat gaaatgtttt aatacatctc atataaaa
//
LOCUS       DAZFRJ010000254         4932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000254 DAZFRJ010000000
VERSION     DAZFRJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.256"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..882)
                     /locus_tag="ACHKUN_004394"
     CDS             complement(<1..882)
                     /locus_tag="ACHKUN_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO6662523.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVID"
     gene            complement(894..2108)
                     /gene="rspA"
                     /locus_tag="ACHKUN_004395"
     CDS             complement(894..2108)
                     /gene="rspA"
                     /locus_tag="ACHKUN_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO6662524.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2314..2640)
                     /gene="ynfA"
                     /locus_tag="ACHKUN_004396"
     CDS             complement(2314..2640)
                     /gene="ynfA"
                     /locus_tag="ACHKUN_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO6662525.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2775..3116
                     /gene="ynfB"
                     /locus_tag="ACHKUN_004397"
     CDS             2775..3116
                     /gene="ynfB"
                     /locus_tag="ACHKUN_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO6662526.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3151..3711
                     /gene="speG"
                     /locus_tag="ACHKUN_004398"
     CDS             3151..3711
                     /gene="speG"
                     /locus_tag="ACHKUN_004398"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO6662527.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3714..4424)
                     /gene="ynfC"
                     /locus_tag="ACHKUN_004399"
     CDS             complement(3714..4424)
                     /gene="ynfC"
                     /locus_tag="ACHKUN_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO6662528.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4532..4837
                     /gene="ynfD"
                     /locus_tag="ACHKUN_004400"
     CDS             4532..4837
                     /gene="ynfD"
                     /locus_tag="ACHKUN_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO6662529.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1339 a         1267 c         1085 g         1241 t
ORIGIN      
        1 gtcgataaca accggaaatt tatttgcatt taagcgtgaa gagaaaatag agagttcttt
       61 tccggtaatt ccttgctgaa tcacttcaga cggttcactg gagaagccca tcaatacaat
      121 acgtgccgct ggagaagcca gcgttacggc ctctttcagg atagaaggat gacaagccgc
      181 atcgataatt aatgtcggct tgatgccttt ttcagcgaaa atctcgccaa gcggtgtctg
      241 gctgttatta atcgcccagt ctgccccgct ctctttcgct ttttccagtc gttcatcaat
      301 gcgatcggca acaatcacat ttttaacgtt atagacgcct tttaatacct gaacgatcgt
      361 caggccgatt ggaccggcac cataaaccag aacggtatca ttttcagtcg gttgaccatg
      421 accggtaacg ttagccgcaa tggtaaaagg ttcgatcatc accgcatatt gatcggccac
      481 tgcttcagga attttccacg cattttttgc cggaaccacg gcatattcac tgaaaccacc
      541 gtcagcgtgc acacctaata cagcaagtgt cgtacaaacg ttcggcttac ctatagagca
      601 cggatagcaa tgcccacagc tgaccaccgg atcgacagca acgcgctcac cgactctggc
      661 gctttccaca ccttcaccca ccgcatcaat gacgccaaag aattcatgac caatgacgcg
      721 cggatatttc gcaaaaggat tatgcccacg gtaaatatgg ctatctgaac cacaaattcc
      781 ggcaagtttc acttttactc gtacttcacc cgctgacggg gtgggtattt cacgttcgat
      841 aatcgacagt tgattcggtt tttcaattaa aatgcttttc attaccttac tccttaccag
      901 ttccacagcg tgccatcttc cagacgtgcg actggcagat aagcaggttc atagggatat
      961 ttcgccgcca gcttttcatc gaattcgatg ccaagacccg gtttgtctcc cggatgcata
     1021 tagccgttat cgaaagtcca gttgtgcggg aagacttcga gcatttgttc ggaataaccc
     1081 atgtactcct ggacaccgaa attggggacc cacagatcaa agtgcagcgc cgcagccatg
     1141 cagactggtg acaaatcgga aggaccgtgt gagccagtac gtacctgata cagcgaagca
     1201 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
     1261 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
     1321 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
     1381 gcaggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
     1441 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
     1501 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
     1561 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
     1621 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
     1681 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
     1741 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
     1801 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
     1861 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
     1921 atacggtgcg catcgcgacc aataagctgc ggacaaaggt gatcctgcaa ataagaggcc
     1981 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
     2041 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctttacg
     2101 atcttcatgt tcgattcctt gcatcgcttg tcgtgatgca tgaaatctac gcaactgagc
     2161 tactaccata caagcataaa gatcgaaaaa agccggagtg atcacaaaaa aaggcgtata
     2221 tttgcgctgt gaatggttga caaaagatga aatagaatac cttttgtcag ctgacacttc
     2281 ctcttatctt attgataaaa tggatttatg ttcctacgtg cgcccccagc ccgcaacaat
     2341 gatcaacatg ccgcaaagcg caatcaacgc acccgtccag tcataaagag tcagtttcac
     2401 gccatccaca acgcgcagcc acatcaacgc cgtgcagaca taaacgccac cataagccgc
     2461 gtaaacacgc ccactcgccg ctggatgcaa cgttaacaac cagacaaaca gcgccagtga
     2521 aatccccgcc ggaagcaaca gccagatact ggcgtttcgt tttaaccaca accagggcag
     2581 aaagcatcca ataatttcac acagcgcagt agcaaaaaat agtaacgttg ttttaatcat
     2641 ctttgtctct tattgacatc atgtatagtt atagggcgac ataatatcat caatataaac
     2701 accctcctgg tacgttttgc gtccgcagtg gatggtgtag aatcaccttt aatcattcat
     2761 acagggaatg aattatgaaa atcactctca gcaaacgaat cggcctgctc gctattctgc
     2821 tgccttgcgc actggcattg agcacaactg ttcatgccga aactaacaaa ctggtgattg
     2881 agtctggcga cagtgcacaa agccgccagc acgccgctat ggaaaaagag caatggaatg
     2941 acacgcgcaa tctgcgccag aaagtgaata aacgcactga aaaagagtgg gataaagccg
     3001 acgccgcttt tgataaccgc gataaatgtg agcaaagcgc caacatcaat gcctactggg
     3061 agcccaatac tttgcgctgc ctggaccgtc gaactggccg cgttattacc ccctaacctg
     3121 ttattgattt aaggaatgta aggacacgtt atgccaagcg cccacagtgt taagctacgc
     3181 ccgctggagc gtgaagattt acgctatgaa catcaactcg acaacaacgc cagtgtgatg
     3241 cgttactggt ttgaggaacc ctacgaagcc tttgttgaac tctctgatct gtatgataag
     3301 catattcacg atcagagcga acggcgcttt gtggtggaat gtgacggcga aaaagccggt
     3361 ctggtggagc tggtggaaat taaccatgtt catcgccgcg cagaatttca gataattatc
     3421 tccccggagt atcaggggaa aggtctggca acccgtgccg ccaaattagc aatggactat
     3481 ggctttaccg ttctcaatct ctataagctg tatctgatcg ttgataaaga gaatgaaaaa
     3541 gcgattcaca tttaccgcaa gcttggcttt tcggttgaag gtgaattgat gcacgagttc
     3601 tttattaatg gtcaatatcg taatgccatt cgcatgtgta tattccagca tcagtatctg
     3661 gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata gcattaataa
     3721 taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg cgggttttac tccttcatca
     3781 acaatgatta gctgacagtc caccggatta gcgtgactgt catattcaca gctctgtttt
     3841 acattaccaa cccgttgatt attcagtaaa gtaaccgctg tgtaatctaa ttttttgatc
     3901 ggatccgttg atggcgtggc gctgacagat aatgttttgt cgttactttt cgtggtttta
     3961 cccagcggat aaccctgatc atcatagcga tattccatct gcatttgttt gctgctggct
     4021 ttaatcacga agccattatc atcggtttcc caactcaccc cggcagaagg taattctgct
     4081 agctggcatt ttccctgtaa acgtactctc ttctccagcg tctcggcatc acggtaataa
     4141 ttggcgtcca gtaccagagc gaccacggta ttattttcca gatccagtaa ttcgagtgaa
     4201 tcaaaacagc cttcttccga caaagtccca gaaacacgtt tcgtcacttc accttgctca
     4261 tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa atcgaattca
     4321 ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg gtcacatcct
     4381 gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt caccagagag
     4441 tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa tgctatgctt
     4501 aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct gtgccgcgct
     4561 tctgctcgcc ctcgcctcac ccgtggtact cgccgcgccg ggatcctgtg agcgcataca
     4621 gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct tcacgttaag
     4681 tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcggcc attgcgctaa
     4741 tgatacgcat aaaattctct atacccgcac caccagcggt aacgtctctg ctcccgcgca
     4801 gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact gaacgccgga
     4861 tacgacacat ccggcgattt gaacatacaa cataatccca ccttattact catacccttc
     4921 tattgatatg ga
//
LOCUS       DAZFRJ010000255         4926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000255 DAZFRJ010000000
VERSION     DAZFRJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.257"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHKUN_004401"
     CDS             complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHKUN_004401"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO6662530.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHKUN_004402"
                     /gene_synonym="chbF"
     CDS             complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHKUN_004402"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6662531.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHKUN_004403"
     CDS             complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHKUN_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO6662532.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHKUN_004404"
     CDS             complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHKUN_004404"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO6662533.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3595..4926)
                     /gene="chbC"
                     /locus_tag="ACHKUN_004405"
                     /pseudo
     CDS             complement(3595..4926)
                     /gene="chbC"
                     /locus_tag="ACHKUN_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT         1340 a         1301 c         1052 g         1233 t
ORIGIN      
        1 agcgcatcag gcagttttgc gtttgtcagc agtctcaagc ggcgcagtta cgccgccttt
       61 gtaggaatta atcgccggat gcaaggttca cgccgcatct ggcaaacatc ctcacttaca
      121 catcccgata actccccaac cgataaccac gctgagcgat agcacctttc aacgacgctg
      181 atgtcaacac atccagctcc gttaagcgtg ggaaacagta agcactctga cggatagtat
      241 tatcgataaa cgccggatga cacatcacct ccagcgaacg atcaccccga tggcttgcgt
      301 catccagcac ttgcaggaac agtgcctcgc taatctcttc accataaaac gcgctactga
      361 atccctgaga actgcgcaga ttagccggta aatcaccagc gttagatagc ggctgacggt
      421 cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc aacaatcggg aaaatctgcg
      481 ggaacatatg cacatgatga tggctatcaa gatgcgtagg tttgcgtcca aatagctcaa
      541 tgaaacgcaa atactgactg gcaagctcct gagtaatttc ttccagcggt aaagcatctt
      601 cttctgccaa ctgccagatc cattttccca gcacaccatc gcgggttaac cccggcatag
      661 ctgtcagggg cttgcccata gtaaggacaa agtgcatccc tatggccaga ctcggttcat
      721 cacgactcaa ctgcaccgca tggtcaatag cctgcccatt caccagcgcc gtcgttgatg
      781 tgacaatccc attgcgacag gcctcgataa tgccgtagtt ctgacctttg cttaagccaa
      841 aatcatcggc attaacaatc agtaagcgtt ccataatcag cctcggttaa tgtgcttttt
      901 taagctctgc gatgcagtcg gcaaaatttg gcagccattt ctcgtgcgcc agaatcatct
      961 cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa cggactaagg tttagcgcca
     1021 gtaacacatc gttaaattct ccgctaagtg cggcgttgct ggcagcaatc tcgaagcctt
     1081 taatggtgtg aatcagcccc atcactttat catcgaaatg cgtaatgcgc ggatgtggcg
     1141 tcgcgccatc gcgccccagc ttacaggtca tttctaccgc ccagtctgcc ggaatattat
     1201 caatatgccc atgatgcggg atattaacgt aatgttcagc ttgcttgtcg ttgtagatag
     1261 cgttgatcac ttcgcacgct gcatcagagt aataagcccc accgcgctgt tccagttctt
     1321 tcggcttaac tttcaactcc ggatttttat acagctcaaa aagttgtttc tcgactttct
     1381 gtactacctg tgctcgtgcg ccgcctttgt agtattcgcc catttcaata gccagcatct
     1441 ctttctgctt gaagtaatac agcagataag aacatggcag cagatttaac gaacgaatta
     1501 agccctcact aaatggcaga tcgaaaatat tttttacaga ggacgctttt aactgccctg
     1561 acgccacacc atcaagcaat tcggcaaagc gcgacttgcc atttaccagc acatccttaa
     1621 tgaacaccat atggttgagg ccgaacagat cgatagataa atcatcgctg tctttcagca
     1681 tcagaacatc gcgaataaac atcttcatgc cgatcggaat attacacacg ccgataaagc
     1741 gtttaaatcc ggtatgacga taaacggctt cagtgaccat tcccgccggg ttagtgaagt
     1801 taatcaccca tgcattcgga caaagttctt cgacatcttt tacgatgtca aaaatcaccg
     1861 gaatggtacg cagaccttta aacagaccgc ccgcgccgtt ggtttcctga ccaagataac
     1921 catgacttaa tggaatacgt tcatccagtt cgcgcgccgg taattggcct acgcgcagtt
     1981 gggtagtaac gaaatcagca tctttcaatg cttcgcggcg atccagcgtt ttataaagct
     2041 tcatcgggac gccagcgtta tcaatcatcc gttggcagag ttcaaaaatg atatccagtt
     2101 tctctttacc atcttcgaca tccaccagcc ataattcgct gaccggcaat tcgtgataac
     2161 gcttaataaa tccttccagt aactccgggg tatagctgct cccgccacca atagtgacga
     2221 cttttaattt ctggctcata atttctccct tcagtacaga atactgatat ctggcatatc
     2281 tgcccccccg gacataaata atccagcaac aggacagata tgtgaattgt caggtataac
     2341 gacttactga ttaaattcag tcaatttctt acgatagctc ttaggcgtaa aggacgttaa
     2401 tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa acgcaatatc
     2461 cgtcacggaa tagttggtca tttccagttg ttttttggca aaattaatac ggatctcatt
     2521 aataatctgc attggtgttt tgccataata tcgttgagtg gctcgcgtca aatattcctg
     2581 tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact gctctttatc
     2641 atgcatcttt tctaccgtac ttttcagcca ctgcggtaca tcatcaatca cctgttcttc
     2701 gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa actcttccag
     2761 ccctgtttcg cggaaattca atgaagaaat cactgtttcc acataggtga gaaaagcgtt
     2821 attggtccgg tatacctgcg aagcgacaaa gcaataaggc aacaacggca ggtaatgctg
     2881 ctcaaaaaag cgtttactga tcccaacgtt caatatacgc gtggcaccaa actcataaaa
     2941 actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca gtaacacgcg
     3001 cttaccgtta atctcctgga aataacgccc ggttaatacc agagtaaatt cataatagtc
     3061 gtgctgatgc agcccgctga tactctcagt tttgttatag ataaacacat ggaagttttt
     3121 gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg
     3181 catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca gtaatcagtt
     3241 cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc agactcactt
     3301 tcatcttaac ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat
     3361 tcaatgccat ccgtgactga tccatcatgg cttttgctgc agcaaaatcg ccctgcttcg
     3421 cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc ggagttgatg ataagcccca
     3481 tcaccacttc ttccagctct tcagcttctg tttgcgtatc aggaatatta tcgagatcca
     3541 tcatacatcg ttcctctctc ttcttaccgg caagattacc cgtaccggca tcgattaaaa
     3601 tttcagggcg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
     3661 gttagccacc acaacaaagg gcagataaat taaggttgcg atgccaaggt tgaagagtgc
     3721 gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     3781 ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg ctgcgagggt
     3841 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     3901 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     3961 ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca ggatcaggcc
     4021 taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     4081 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     4141 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     4201 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     4261 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     4321 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     4381 aattaatgcc gagaaggaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     4441 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc
     4501 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     4561 atatgggggt gacggtcata aatgctgcaa cggataacaa ccccgccgcc agcgcatcga
     4621 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc
     4681 ctaatgttcc gttatatacg ttgccgccaa tacctttcag gccattaagt gtttcaatgg
     4741 ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa
     4801 aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa
     4861 agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga
     4921 gtacct
//
LOCUS       DAZFRJ010000256         4837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000256 DAZFRJ010000000
VERSION     DAZFRJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.258"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(245..621)
                     /gene="rnpB"
                     /locus_tag="ACHKUN_004406"
     ncRNA           complement(245..621)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKUN_004406"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(654..1770)
                     /gene="garK"
                     /locus_tag="ACHKUN_004407"
                     /pseudo
     CDS             complement(654..1770)
                     /gene="garK"
                     /locus_tag="ACHKUN_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(1867..2757)
                     /gene="garR"
                     /locus_tag="ACHKUN_004408"
     CDS             complement(1867..2757)
                     /gene="garR"
                     /locus_tag="ACHKUN_004408"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6662534.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2787..3557)
                     /gene="garL"
                     /locus_tag="ACHKUN_004409"
     CDS             complement(2787..3557)
                     /gene="garL"
                     /locus_tag="ACHKUN_004409"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO6662535.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3573..4835)
                     /gene="garP"
                     /locus_tag="ACHKUN_004410"
     CDS             complement(3573..4835)
                     /gene="garP"
                     /locus_tag="ACHKUN_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO6662536.1"
                     /translation="MVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLL
                     MQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSE
                     APSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVM
                     GVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHY
                     IKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGF
                     AGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAF
                     FGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAAL
                     VFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1150 a         1399 c         1175 g         1113 t
ORIGIN      
        1 catctctgca atatattgaa tttgcgtgct tttgtaggca ggataaggcg ttcacgccgc
       61 atccggcatg aataaagcgc actcaacaat ctgaaacccg ccggaacggt ttattacgta
      121 catcaggtga aactgaccga taagccgctt tcttttgggt atagcgtcgt ggacagtcat
      181 tcatctttct gcccctccaa aagcaaaaac ccgccggttt acgctggcgg gttttcgtta
      241 aatcaggtga aactgaccga taagccgggt tctgtcgtgg acagtcattc atctaggcca
      301 gcaatcgctc actggctcaa gcagcctacc cgggttcagt acgggccgta ccttatgaac
      361 ccctatttgg ccttgctccg ggtggagttt accgtgccac ggactgttac cagccgcgcg
      421 gtgcgctctt accgcaccct ttcaccctta cctgatcccg cttgcgcggg ccatcggcgg
      481 tttgctctct gttgcactgg tcgtgggttt cccccccagg cgttacctgg caccctgccc
      541 tatggagccc ggactttcct cccctccgcc cgtctccccc gaagaggacg acgacgaagc
      601 ggcgactgtc tggtcagctt cggcgcgcag tatagagggt ttgcgcgccc ttgtcacccc
      661 gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac gtgaagcacg gcagatattg
      721 tcataagccc cgcggaatgc ttcgtccaac gtaccaatgc tggtcagtac gctgaagaca
      781 gcatcaatgc catgctgatg tacaacgcca acatcatcgg tcaggctacc cgcaatgcca
      841 atcaccggtt tatggtactt cttcgccacg tttgcgacgc caatcggtac cttgccgtga
      901 atactctggc tgtcaatacg cccttcaccg gtgatcacca gcgtacaatc gtgaatatgc
      961 tcctccagat tcagcgccgt agtgacgatt tcaataccac ttttcagttc cgcaccaaga
     1021 aacgccatta gcgccgggga catctttcac atcaacatgc agcgcttttt taatgacctc
     1081 ggcatagtga gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg
     1141 tgggccaaag atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc
     1201 gacgcgaatg acgcaatctt ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag
     1261 agtattaaga ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc
     1321 ccccagcgcc tgtaccatgc ctgcgccgcc atcatttgta gcgctaccgc caatgccgat
     1381 aataatgttt gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga
     1441 agtggtcacg agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc
     1501 catttcaata aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc
     1561 gcccagcggc cctgtaaccc aggcgtgacg ttcagaaccc tgggtggctg caatcatcgc
     1621 ttccaccgtt ccttcgccac cgtcggcaac cggaatagaa acgtactgtg catcaggaaa
     1681 aatttcccga aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt
     1741 ataagagtct ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag
     1801 ggcgtttctc ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg
     1861 acgtcattaa cgagtaactt cgactttcgc cagtttttcg tagtagcacg ccagggcgct
     1921 atgatccgcc gttcctaaac catctgctcg cagtgcctgc atcatctcca taaccgcagc
     1981 tgtgagcggc agttgcgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt
     2041 aatatgcaga tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt
     2101 ggcatccagc acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg
     2161 gttaacgccc gctttggttg ccagcgttag cgcttctgac atcgcggcaa tattcagcgc
     2221 cacaatgacc tgatttgcca gtttggtgac gttacctgca ccgatttccc cggtatgcac
     2281 cacggaaccc gccatcgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc
     2341 gcccaccatc actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc
     2401 atccagcata tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg
     2461 tgcgatagaa ctcatatcga tcaataccgt acctggcttc gcgccttcaa taatgccatt
     2521 ctcacccagc gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc
     2581 gcactgttca gcgatcgctt tagccgtaga cgctgtttct gcacctgcag caatcacgtc
     2641 agcaatagct tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt
     2701 tttactcatt ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc
     2761 aatcatctct cttgttgcgg tggtggttat tttttaaagg tatcagccag tttctgagtg
     2821 gcagagcgga agacgccaag atcgctgccg acagccacaa acgtcgcgcc ccattccaga
     2881 taacgacgcg catcggcttc gaccggcgcg agaataccgc tgggtttgcc gtgcgcactg
     2941 gcacggttaa aaatgtgctg aattgctttt tgtacatccg ggtgcgaggc attgccgaga
     3001 tggcctaatg ccgcagccag atcgctgggg ccgacgaaga taccgtctac accttcggta
     3061 gcggcaatgg catcgacgtt atctacgccc tgctgacttt ctatctggac cagaatggtg
     3121 atgttcttgt tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga
     3181 gaaacggaga cgccgcgaat gccttccggt gggtaacggg ttgatgccac cgcctgctct
     3241 gcttcctctt ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga
     3301 cgcttaataa ttaccggctc gttggtcggc actcgcacta ctggcgcgct ggcgctgcct
     3361 ttcagggcca ttaactgcgg aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc
     3421 agcaccagcc agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt
     3481 gctgaccagc aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg
     3541 aaaacatcgt tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg
     3601 tcgccaacta cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc
     3661 agtgctgcat tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca
     3721 atggaggcaa cattgccaaa gacgttaaag acgccgccac agaggccaac aatctctttc
     3781 ggcgcggtgt cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc
     3841 agcgccatca gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg
     3901 gaagccagca acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg
     3961 cgtttgatca gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt
     4021 gccggaatcg aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga
     4081 taaatcggga accaggtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa
     4141 aatacgccca acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgggaccg
     4201 cttgctgccg cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag
     4261 ataaacttca gctcttccgc agacatacgt gggtgatctg tcgggttatg aatcaatttg
     4321 atccacagcg ccgtcagcac aaaaccaatc acccccataa cggtaaagac gtgctcccag
     4381 ccccaggcga aagtcagcca gccaagcaga ggcgaaaaga gcgccagcga gaaatattgc
     4441 gccgagttaa agatggcgga ggcagtacca cgttctttcg tcgggaacca ggcggcgaca
     4501 atacgggcgt tcgccgggaa tgatggcgct tccgagaagc cgagcataaa gcgcataaag
     4561 aacatggaga tccctgccca ggccagcggg aacatatcaa caaagccttg caggaaagtg
     4621 aatagcgacc agaaaaagag gctataggta taaacttttt tcgagccaaa cttatcaagc
     4681 agccagccgc cggggatttg catcagcaag taggcccagc caaaagcaga aaagatgtaa
     4741 cccatcgaaa ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc
     4801 gttgcacgat cggcgtagtt aacggcggta acaataa
//
LOCUS       DAZFRJ010000257         4805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000257 DAZFRJ010000000
VERSION     DAZFRJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.259"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..851
                     /locus_tag="ACHKUN_004411"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHKUN_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHKUN_004412"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHKUN_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHKUN_004413"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHKUN_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3168..3578)
                     /locus_tag="ACHKUN_004414"
     CDS             complement(3168..3578)
                     /locus_tag="ACHKUN_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO6662537.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            3764..4732
                     /locus_tag="ACHKUN_004415"
     CDS             3764..4732
                     /locus_tag="ACHKUN_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO6662538.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT         1183 a         1260 c         1164 g         1198 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgcag ccccccctcc ctgctgtata ccccagtacc acaacgcggg
     3241 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     3301 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     3361 gggtcaagcc aggtgcatcg cagcaggatt ttcgcagcaa cagtcatctg gtcatgttca
     3421 aaccacggcg gttgtccgtc atttccccgc ttaagtgtta acggacaacc acaggacaca
     3481 cagcacaaaa cgtcctgtcg gttgtgcttc gcggcttctt tcgccgtaac aaaatggcct
     3541 ttgctgtcat tcgccatata gcagttcaga attcgcatat ctcctccttg tatcttgaca
     3601 attggttaaa ataactgtga ttagatttgg caaggtgtat gcagcccgat ccctcaaggg
     3661 gttcatagcc cgtagctcag cgaatggcgc atacactgag ccactcatct gaaacccgaa
     3721 cagttgtcag tacgccataa cgcatttgca aggatgggta atcatgagtc agccaggttc
     3781 actttgtgtg gatatcgatg tttcgaaagc gatactggat attgccgcca gcagcgctat
     3841 tgaacaattt tctgtcggca atgacagtga tggctttgac gccatcattg ctgagttgag
     3901 aatgtacgcc attgcgctgg ttttaatgaa agccactgga ggacttgaaa ccgccatagt
     3961 ctgtgcgctt caggcagaag gttttgagct tgccgtggtt aaccccagac aagctcgtga
     4021 ctttgcccgc gccatgggat acctcgcgaa aactgatagt attgatgcca gagtgctttc
     4081 ccagatggct gaggttctca accggcatcc ggagcgggaa cgctttatca gagcactgcc
     4141 ggatactgaa tgtcaggttc tcactgcaat ggtagttcgt aggcggcagt tgattacgat
     4201 gctggttgcc gagagaaacc ggttgcaccc tgctcatcca ttgaacagga aaagcattaa
     4261 tatcatcatc aaagcactgg aagacgagct tgcgcgacta gatgctgaca tgaacagcca
     4321 tatccatagc cacttcaaag agcttgctaa gcgactgagc agtattaaag gtgttggcac
     4381 catgacggct gctgcgctgc tggcggaagt acccgaacag ggtaacctct ccaggcgtga
     4441 aatcagtgtg cttgtcggta tggttccagt aaaccgggac tcgggcacga tgagagggcg
     4501 acgaaccatc tttggcggca gggctggtgt gcgaacagcc ctgtacatgg cagcgcttgt
     4561 cgcaacccgc tttaacccgg taataaaggt gttttacatg cgactgcttg ccgcaggaaa
     4621 acccaaaaaa gtggcgctgg ttacctgtat acgcaagctg ctgactatcc tgaacgcaat
     4681 gctcagaaag aacgaagaat ggaatgaatc gtaccatcac gttgctccat aatttttacg
     4741 ttcaagacag ttgctgagct gtccggggaa gactgaaacc tccggtctgg cagtgtgtgc
     4801 agtac
//
LOCUS       DAZFRJ010000258         4788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000258 DAZFRJ010000000
VERSION     DAZFRJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.260"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            210..1109
                     /gene="yegS"
                     /locus_tag="ACHKUN_004416"
     CDS             210..1109
                     /gene="yegS"
                     /locus_tag="ACHKUN_004416"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO6662539.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1191..1964)
                     /locus_tag="ACHKUN_004417"
     CDS             complement(1191..1964)
                     /locus_tag="ACHKUN_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6662540.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2070..3110)
                     /gene="gatD"
                     /locus_tag="ACHKUN_004418"
                     /pseudo
     CDS             complement(2070..3110)
                     /gene="gatD"
                     /locus_tag="ACHKUN_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(3158..4513)
                     /locus_tag="ACHKUN_004419"
     CDS             complement(3158..4513)
                     /locus_tag="ACHKUN_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO6662541.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4517..4786)
                     /gene="gatB"
                     /locus_tag="ACHKUN_004420"
     CDS             complement(4517..4786)
                     /gene="gatB"
                     /locus_tag="ACHKUN_004420"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO6662542.1"
                     /translation="MVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEIETYMD
                     GVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
BASE COUNT         1304 a         1252 c         1064 g         1168 t
ORIGIN      
        1 ggttgaatat taggcatatg ctcttttttg aaatttatcg gtggcagccg ttagtattcg
       61 ctgtccccat tgcaagctcc tggtggtaac cactgaatcc tccatacttg aactgacttt
      121 ttatcctccg actttcatcc tgttctgact ccaccttttg ttttctgctc tacactatct
      181 acagaccaat cataaaggca catacgatca tggcagaatt tcccgccagc ttactgattc
      241 ttaatggcaa aagtactgac aatctaccct tgcgcgaagc aattatgctg ttgcgtgagg
      301 aaggaatgac gatccatgtg cgggtcacct gggagaaagg cgatgccgca cgatatgtag
      361 aggaggcccg gaagttgggc gtcgcaacgg tgattgccgg tggtggcgat ggcaccatta
      421 atgaagtttc tacggcgttg attcagtgtg agggggatga catacccgcg ctgggaattt
      481 tgccattagg aaccgccaat gattttgcca ccagtgtagg gattcctgag gcactggata
      541 aggcgctgaa actggcaatt gccggtaacg ccattgcgat agatatggcg caggtcaaca
      601 aacaaacctg ttttattaat atggcgacag gcggatttgg gacgcgtatt accacagaaa
      661 cgccggaaaa attaaaagcc gcgctgggtg gcgtctctta catcattcat ggcttaatgc
      721 gcatggatac tctgcaaccg gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag
      781 gtgacgccct ggtcattggt attggtaacg ggcgtcaggc cggtggcggt cagcaattgt
      841 gtccgaacgc gttaattaac gatggcttgc tgcaactgcg catttttacc ggcgatgaaa
      901 ttcttccggc tctcgtatca accttaaaat ctgacgaaga taacccgaat attatcgaag
      961 gcgcttcgtc gtggtttgat atacaagccc cacacgaaat cacttttaat cttgatggcg
     1021 aaccgttgag cggacaaaac tttcatattg aaatacttcc ggcggcgttg cgttgtcgat
     1081 taccaccaga ttgtccatta ttgcgttaat caaactattc tctcttgaac cagggagaat
     1141 ccgaaatgcc ggatgcggtg taaatacctt atccggcata cagaacaata ctacaccgta
     1201 attaatttta cccgtgtctt ctgcaatgcc agtttatcca cctctgatac agcatcatcg
     1261 gtaataataa tgtcaatttt ttcgattggc agtacctgat taaaaccgcg acggttaaat
     1321 ttcgatgaat cgagaacggc aatcacttta ttagcggcgg tgaccatcgc accgctaatc
     1381 gaataacctt cattaaaggt cgtaatacca ttaaccgcat caataccatc agcaccgaca
     1441 aacattaaat cggcattaat atcctgcagg gaacgttcag caatagatcc atgcatagat
     1501 cgcgttttat ggcggaccgt gccaccgcag acgaccagag taatgtcttt attttcggaa
     1561 agggcaaacg cagccgggag actgttggta atcaccgtga tatttttggc ggtcattaat
     1621 ccttcggcga taagcattgt ggtacttccg ctgtcgagaa taacggtcat cccttcatgg
     1681 atcattttga ctgccgcctg cgctattctg tttttcggcg cgctggcgag ctgaaatcgc
     1741 tctttaaacc cgacttcctg ggtctcggtt tcactattac cggacattat tttcgccgca
     1801 ccgccatgaa agcgcgtaac cacgcctttt tgttcgagaa agcgcaaatc ggcacggatt
     1861 gtcgcttccg aggcagcaaa tactcccgcc agatcctgaa caagcacggt tccctgttca
     1921 ttcactaatt ggatgatctt attccttcgc tcgaatgaat tcatcgtcat ttacccatat
     1981 tcaattgtgg ctagtgtaaa cgaaggggga ccattggtga atgatctgtt ttgcacaata
     2041 ccgggccagc atcacgctgg cccgcggttt cagggaatga gcaacacttt gcccggcata
     2101 gcattacgag cgatgtcacg caccgcctgg gcgaagcttt caaagcttcc acggtgagcg
     2161 attaatggct ccaggcttaa cttacgttct gtcagcaacc ggctcgccgt ttcccactcc
     2221 tgccccggcc aagggctgga gtagttcatc caactgccga taaccgtcag ctctttacgc
     2281 aatattttgc caaacgttgc cgatgttaaa tgcagatcct gatgcaacgt gcccaccagc
     2341 gccagttggg catgaggacc ggcaatctct accgccagtt cgacagtttg cggtacgcca
     2401 gccgtctcga ggataagctg attaaagcgc agttagcgta aaacgctctg catttgcggc
     2461 gcgctcattt cgctactgtt aaatgtttgc atcgcaccga aagattttgc cagtgccagt
     2521 ttttctgaac taatgtcgat cgccgtcaca ctctttgctc ccagcgcgac agcgcactga
     2581 atggccagca ggccaatggt tccggcacca ataataataa cgtttttatt ctcacaacct
     2641 tgcgctaaat gaaaagcatg caggccaacg gtaatcggtt caataaaagc cccatcctca
     2701 ataggcatat cagtaggtag agcaaagaca tttttccgct taacgacaat atattcggca
     2761 aatccaccat aacgccgtga gccaataaaa tcatattttg cgcactggga ataaaaccct
     2821 ttcaaacact ctggacaagt aaaacagggt aataacggca cacaggcaac cgcatcgcca
     2881 ggatgtaaat catcaacacc ggatcccacc gcatcaatat agccgctaaa ttcatggcct
     2941 aacgttattg gataataatg tgcaccattt ttaaatatcc tgggtaaatc ggaaccacat
     3001 aagcccgagc tggcaatttt tacccgcacc tcatcctgat gtttaatttc aggaatgacg
     3061 ctttctgcaa cgcgcacgat accatcagta tcattcacca ctgatttcat aaaaactcct
     3121 gattgttaag ggggataacc tccccctcag gtaaaaatta ttctgcgaga acgactttct
     3181 cttgtttaat aaagccacgc gctctacgcc aggtcatgaa aataccggta agataaattg
     3241 cgccgataat aatgaatccg ggaatatttt gcggggagaa aacctgaatc agtaaccagg
     3301 taatgggaga accgccctga tccattgaag ccaccatacc cccggctttt aacgccccag
     3361 cattagccgc cagttgggtg tgcaaaccaa tagtttgcgt cgcgatccac agagtgatgc
     3421 tcatgatgat gacacccgag attaaggtgc ggaacagatt tccacgatgc acggcgactg
     3481 ccatcgccac aaagaagccg atggtggcaa gatcgccaaa cggcagcacc tgattacccg
     3541 gaacacaaac agcaattaaa atggtgagcg ggataaaaat caggcttgcc gataccaccg
     3601 ccgtatgccc cagcagtaat gcgggatcca ggccaatcag gaactcctga ccgccgaact
     3661 tcgcctgtaa acgactacgg gcctgcttag cgatgggcgt taaaccatcc atgatgggtt
     3721 taatcacccg tggcattagc agcatcactg ccgccgtttt taccgccagc tgcaatacac
     3781 ctttgacatc gtaacccgcg aggatgccga taatcagccc catcacaaaa cccacggtga
     3841 caggctcgcc aaatggacca aatttgcgct gaatatcgtc ggcgctaaat ttaattcggt
     3901 taacgcctgg gattttctcg atgatagcat cgaccagcac cgcaatcggc cccatatacg
     3961 ccgacgtacc gtgcggaata gcaatacctt ccagctcaaa gaaatttcgg gtatcgcggg
     4021 caaaccagtc gccgagctta taaacaaacg ccgcgtgaat taccacacct gccatcccta
     4081 tcatccatga accggttgcc aggtgcagca acgcgccggt gaaggtcata tgccagatat
     4141 tccagatatc aacatttacc acccgcgtca tacgggtcag taacatcgcc acgttaacca
     4201 atatcgcaat cggaatcgcc accagcgcaa tttgcgacgc ccaggtcatt ggtgaagagc
     4261 ccggccagcc gacatcgacc acatgcagat tcaggtcgaa attttccgcc atcgctttcg
     4321 ccgccggacc aatggaatcc agcattaagc caatcacaag gccaatgcca acaaagccaa
     4381 tcccgatatg cagacccgct ttaaagcaat cgcctgcctt catgcctaat attttagaaa
     4441 aaataatgat gacaatcgga agcatcaccg tagggccgag gtcgagaata taacgcatga
     4501 cttctgaaaa cataggtcac ccctgtaaga tagtcagaat tttattttgt aatgcttcga
     4561 taccgacacc agaaataaaa ggcatgccgt gaactaacgg aatatcgcca aaactacgat
     4621 ccactttggc agtggtgcat atcaaatgca caccatccat ataggtttct atttcattaa
     4681 cccgacactg gattaattca acaggaatat tatgattctg acacaactct ttaatttctt
     4741 ccgccgccat cgtagaggtc gcaaccgcgc ctccgcaagc gacaataa
//
LOCUS       DAZFRJ010000259         4782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000259 DAZFRJ010000000
VERSION     DAZFRJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.261"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            404..4306
                     /gene="espP"
                     /locus_tag="ACHKUN_004421"
     CDS             404..4306
                     /gene="espP"
                     /locus_tag="ACHKUN_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO6662543.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVIARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4554..>4782)
                     /locus_tag="ACHKUN_004422"
     CDS             complement(4554..>4782)
                     /locus_tag="ACHKUN_004422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662544.1"
                     /translation="AQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLFF
                     VDKESGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1470 a         1070 c         1052 g         1190 t
ORIGIN      
        1 ataaatattg aatacattta ctgagcatgc aaaagaaaaa gacaaactaa tcccggcctt
       61 tcacagaatt tcctctatca gaggctgagt tgacaacctg ctcacccagg aaatagtaac
      121 acccgaaaac atgaagagac tctgataaac aatctcgata catattcctc atctttcaca
      181 gcatctcact tctttaacag gaattattat tatctacgcc tcctcctttc atgtatccct
      241 catgaatgat taagataaac atactgtgtt tgttatccgt atgataacaa acacataaag
      301 gagtaaacat ttatctggat gatgattaca atgttcacga ctgtcagaga cacacctctg
      361 atacattaga aaaaccaatg tttcccttaa aaatggagct tatatgaata aaatatactc
      421 tcttaaatac agccatatta caggagggtt aatcgctgtt tctgaattat ccggcagagt
      481 atcatcaaga gcaactggta agaaaaaaca caaacgcata cttgcattat gttttttagg
      541 cttattacaa tcctcatatt cttttgcgtc acagatggat atttcaaatt tctacatccg
      601 tgactatatg gattttgcac agaacaaggg catatttcag gctggcgcaa caaatattga
      661 aatagtgaag aaagatggct ccaccctgaa actaccggaa gtaccatttc ctgacttctc
      721 accggttgca aacaaagggt caaccacatc tattggtggt gcatacagta tcacagccac
      781 acacaatacg aaaaaccacc actcagttgc gacgcaaaac tgggggaaca gcacgtacaa
      841 acaaactgac tggaatactt cacatcctga ttttgcagta tcccgacttg acaagtttgt
      901 tgttgagacc cgaggtgcga ctgaaggcgc agatatttcg ttatcaaaac agcaggcact
      961 tgaacgttac ggggttaatt ataaaggaga aaagaaactt atcgcattca gagccggctc
     1021 tggtgtggta tccgttaaaa aaaatggacg cataactcca tttaatgagg tttcttataa
     1081 gccagaaatg ttaaatggct ctttcgttca cattgatgac tggagtggat ggctgatatt
     1141 aaccaacaac cagtttgatg agtttaataa cattgcctct cagggtgaca gcggttcagc
     1201 actgttcgtc tatgataacc aaaagaaaaa gtgggttgtc gctggaactg tctgggggat
     1261 ttataattac gccaatggca aaaaccacgc agcatacagt aaatggaacc agacaaccat
     1321 tgacaacctg aagaacaagt attcttacaa cgtggatatg tcaggagctc aggttgcaac
     1381 cattgaaaat ggaaaactga caggcactgg ctcagacacc accgatataa aaaataagga
     1441 cttaatattt actggcggtg gagatatcct cctgaaatcc tcttttgata atggtgctgg
     1501 cggtcttgtc tttaatgata aaaagaccta tcgagtaaac ggggatgatt tcacctttaa
     1561 aggtgccggt gttgatacaa gaaacggcag caccgttgag tggaatatcc ggtatgataa
     1621 taaagacaac cttcacaaaa ttggtgatgg cacattagat gtccgaaaaa cccagaacac
     1681 caacctgaaa acaggtgagg gtcttgtcat tcttggagct gaaaaaacat tcaataatat
     1741 ctacataacc agtggtgatg gaactgtccg actgaatgca gaaaatgcac tgtctggcgg
     1801 tgaatacaac ggtattttct ttgcgaaaaa tggcggaact cttgacctga acggatataa
     1861 tcagtctttc aataaaattg ctgcaactga ttcaggtgct gtaataacca atacgtcaac
     1921 caaaaaatcc attttatccc tgaataatac tgctgactat atctatcacg gcaacataaa
     1981 cgggaatctg gacgtacttc agcatcatga gacgaaaaaa gagaaccgtc gtcttattct
     2041 tgatgggggc gtggacacaa caaatgatat aagcctgcgt aatacacaac tgtccatgca
     2101 gggacatgcc actgaacatg ccatttatcg ggatggagct ttctcttgtt cactaccagc
     2161 tcctatgcgc tttttgtgtg gcagtgatta tgttgcagga atgcaaaata cagaagctga
     2221 tgctgtaaaa caaaacggaa atgcctataa aaccaacaat gctgtctctg atttatcgca
     2281 gccagactgg gaaaccggaa cattcagatt tggaacgcta catcttgaaa attccgattt
     2341 ttctgttggt cgtaatgcaa atgtaatcgg ggacattcag gccagtaaat caaacattac
     2401 tattggtgac actacagcat atattgattt gcatgctggt aaaaatatta ccggtgatgg
     2461 ttttggcttc cgccagaata ttgtgcgtgg aaactcacaa ggagaaacgc tgtttacagg
     2521 agggatcaca gcagaagaca gcactatcgt tattaaagat aaagcaaaag cattattttc
     2581 aaattatgta tacctgctga acacaaaagc aaccatagag aacggtgctg atgtgacaac
     2641 tcaaagtggt atgttctcca cgagcgatat cagcatctct ggtaatctgt ccatgacagg
     2701 caatcccgac aaagacaata aattcgagcc ctcaatatat ctgaatgatg cttcttatct
     2761 actgactgac gactccgcca gactcgttgc caaaaataaa gcatctgtgg tgggagatat
     2821 acactccact aaaagtgcat ccatcatgtt tggtcatgat gaaagcgacc tctcgcagtt
     2881 gtctgacaga acctcaaaag ggcttgcact tggtctttta ggtggctttg atgtctcata
     2941 tcgcggttca gtcaatgccc cgtcagcatc tgccactatg aacaatacct ggtggcaact
     3001 aaccggagat tctgcgctga aaacactgaa aagtacaaac agcatggtct atttcactga
     3061 cagcgcaaac aataagaaat tccatacgct gacggtcgat gagctggcaa ccagcaacag
     3121 cgcctatgcg atgcgtacaa acctttctga atcagacaaa ctggaggtca aaaaacacct
     3181 gtctggtgag aacaatattt tactcgttga tttccttcag aaaccaacgc ctgaaaaaca
     3241 actgaatatt gaactggtaa gcgcgccaaa agacaccaat gaaaatgtct ttaaagccag
     3301 caaacaaacc attggtttca gtgatgtaac gccggtcatt acaaccaggg aaaccgatga
     3361 caaaataaca tggtcactga caggctataa cacggtagca aacaaggaag caacccggaa
     3421 tgccgccgcc ctgttctctg ttgactataa agcgtttctg aacgaggtca acaacctgaa
     3481 caaacgtatg ggtgacctgc gtgatatcaa cggcgaagcc ggtgcatggg cacgcatcat
     3541 gagcggtacc ggctctgcca gtggtggttt cagtgacaac tacacgcacg ttcaggtcgg
     3601 ggtcgacaaa aaacacgagc tggacggact ggatttgttt accggtttca ctgtcacaca
     3661 cactgacagc agtgcctccg ccgatgtttt cagtggtaaa acgaagtctg tgggggctgg
     3721 cctgtatgct tccgccatgt ttgattccgg tgcctatatc gacctgattg gcaagtatgt
     3781 tcaccatgat aatgagtaca ctgcaacctt tgccggactc ggaacccgtg attacagcac
     3841 gcattcatgg tatgccggtg cagaagcggg ctaccgctat catgtcactg aggatgcctg
     3901 gattgagcca caggctgagc tggtttacgg ttctgtatcc ggtaaacagt ttgcatggaa
     3961 ggaccaggga atgcatctgt ccatgaagga caaggactac aatccgctga ttggccgaac
     4021 gggtgtggat gtgggtaaat ccttctctgg taaggactgg aaagtgatag cccgtgccgg
     4081 tctgggctac cagttcgacc tgctggctaa cggcgaaacc gtattgcggg atgcatctgg
     4141 tgaaaaacgc atcaaaggtg aaaaggacag ccgtatgctg atgtccgttg gcctgaatgc
     4201 agaaatcagg gataacgtcc gctttggact ggagtttgag aaatccgcct ttggtaagta
     4261 caacgttgat aatgcagtca acgctaattt ccgttactcg ttctgatact ccgtattcaa
     4321 aagggcctgc gggcccttta cgctcagtgc ctgccgccct gatatgtctg agagacattt
     4381 ccgggatgtc agtcgactgg tttacttaac tgcgatgact aaagctggca gtaatgccgg
     4441 cttttttacg gccacatacg tcttcctctc atccggaact gtgatacaaa tcctgcatac
     4501 aggattgacc gccggttcct gcaaatggca cattttcagt aagatagcga aggttacatt
     4561 cccccgtcat gtgtggcaca agctgtcacc gggaaagaaa cggaaagcac atgaaaaaca
     4621 tcaaaactca caccggactc cttatccaca aagaacaaac gcggcgcgtt cgtcttcatg
     4681 aaacacccac tgcctggtgc cacacgcacc gggaatgcta cagcaaaacg accggtcgcc
     4741 gctgcggcag ccctgacagt ctcagcaggc tgattctgag ca
//
LOCUS       DAZFRJ010000260         4777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000260 DAZFRJ010000000
VERSION     DAZFRJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.262"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHKUN_004423"
     CDS             complement(<1..596)
                     /locus_tag="ACHKUN_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6662545.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHKUN_004424"
     tRNA            complement(711..786)
                     /locus_tag="ACHKUN_004424"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHKUN_004425"
     tRNA            complement(793..867)
                     /locus_tag="ACHKUN_004425"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="ACHKUN_004426"
     tRNA            complement(984..1068)
                     /locus_tag="ACHKUN_004426"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="ACHKUN_004427"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHKUN_004427"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKUN_004428"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="ACHKUN_004428"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO6662546.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKUN_004429"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHKUN_004429"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO6662547.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKUN_004430"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHKUN_004430"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO6662548.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1210 a         1218 c         1086 g         1263 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagca caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgttta
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4681 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4741 aacgaaaggc ccagtctttc gactgagcct ttcgttt
//
LOCUS       DAZFRJ010000261         4747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000261 DAZFRJ010000000
VERSION     DAZFRJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.263"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            51..1013
                     /gene="tauA"
                     /locus_tag="ACHKUN_004431"
     CDS             51..1013
                     /gene="tauA"
                     /locus_tag="ACHKUN_004431"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6662549.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1026..1793
                     /gene="tauB"
                     /locus_tag="ACHKUN_004432"
     CDS             1026..1793
                     /gene="tauB"
                     /locus_tag="ACHKUN_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO6662550.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1790..2617
                     /gene="tauC"
                     /locus_tag="ACHKUN_004433"
     CDS             1790..2617
                     /gene="tauC"
                     /locus_tag="ACHKUN_004433"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO6662551.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2614..3465
                     /gene="tauD"
                     /locus_tag="ACHKUN_004434"
     CDS             2614..3465
                     /gene="tauD"
                     /locus_tag="ACHKUN_004434"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO6662552.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3571..4545)
                     /gene="hemB"
                     /locus_tag="ACHKUN_004435"
     CDS             complement(3571..4545)
                     /gene="hemB"
                     /locus_tag="ACHKUN_004435"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO6662553.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1054 a         1226 c         1385 g         1082 t
ORIGIN      
        1 tttttattcc tgaatactgc tcacataaca agacagggga gcagacaatc atggcaattt
       61 catcgcgtaa cacacttctt gccgcactgg cattcatcgc ttttcaggca caggcggtga
      121 acgtcaccgt tgcgtatcaa acctcagccg aaccggcgaa agtggctcag gccgacaaca
      181 cctttgctaa agaaagcgga gcaaccgtgg actggcgtaa gtttgacagc ggagccagca
      241 tcgtgcgggc gctggcttct ggcgatgtgc aaatcggcaa cctcggttcc agcccgttag
      301 cggttgcagc cagccaacag gtgccgattg aagttttctt gctggcgtca aaactgggta
      361 actccgaagc gctggtggta aagaaaacta tcagcaaacc ggaagatctg attggcaaac
      421 gcatcgccgt accgtttatc tctaccaccc actacagcct gctggcggcg ctgaaacact
      481 ggggcattaa acccgggcaa gtggagattg tgaacctgca gccgcccgcg attatcgctg
      541 cctggcagcg gggagatatt gatggtgctt atgtctgggc accggcggtt aacgccctgg
      601 aaaaagacgg caaggtgctg accgattctg aacaggtcgg gcagtggggt gcgccaacgc
      661 tggacgtctg ggtggtgcgc aaagattttg ccgagaaaca tcctgaggtc gtgaaagcgt
      721 tcgctaaaag cgccatcgat gctcagcaac cgtacattgc taacccagac gcgtggctga
      781 aacagccgga aaacatcagc aaactggcgc gtttaagcgg cgtgccggaa ggtgacgttc
      841 cggggctggt gaaggggaat acctatctga cgccgcagca acaaacggca gaactgaccg
      901 gaccggtgaa caaagcgatc atcgacaccg cgcagttttt gaaagagcag ggcaaggtcc
      961 cggctgtagc gaatgattac agccagtacg ttacctcgcg cttcgtgcaa taaaaggagg
     1021 cgcagatgct gcaaatctct catctttacg ccgattatgg cggcaaaccg gcactggaag
     1081 atatcaacct gacgctggaa agcggcgagc tactggtggt gctggggccg tccggctgcg
     1141 gtaaaaccac cctgctgaat ctgattgccg gttttgtgcc ttatcagcat ggcagcattc
     1201 aactggcggg taagcgtatt gagggaccgg gagcagagcg cggggtggtt tttcagaatg
     1261 aagggctgct accgtggcgc aatgtacagg acaacgtggc gttcggcctg caattggcag
     1321 gtatagagaa aatgcagcga ctggaaatcg cgcaccagat gctgaaaaaa gtggggctgg
     1381 aaggcgcaga aaaacgctat atctggcagc tttccggtgg tcaacgtcag cgggtgggga
     1441 ttgcccgcgc gctggcggcg aatccccagc tgttattact cgacgagccg tttggtgcgc
     1501 tggacgcctt cacccgcgac cagatgcaaa ccctgctgct gaaactctgg caggagacgg
     1561 gcaagcaggt gctgttgatt acccacgata tagaagaagc ggtgtttatg gcgactgaac
     1621 tggttctgct ttcacccggc cctggccgcg tgctggagcg gctgccgctc aactttgctc
     1681 gccgctttgt tgcgggagag tcgagccgca gcatcaagtc cgatccacaa ttcatcgcca
     1741 tgcgcgaata tgttttaagc cgcgtatttg agcaacggga ggcgttctca tgagtgtgct
     1801 cattaatgaa aaactgcatt cgcggcggct gaaatggcgc tggccgctct cgcgtcaggt
     1861 gaccttaagc attggcacgt tagcggtttt actcactgta tggtggacgg tggcggcgct
     1921 gcaactgatt agcccgctgt ttttgccgcc gccgcaacaa gtactggcaa aactgctcac
     1981 cattgccgga ccgcagggat ttatggacgc cacgctgtgg cagcatctgg cagccagcct
     2041 gacgcgcatt gtgctggcgc tactcgcggc ggtggtgatc ggtattccgg tcgggatcgc
     2101 catggggcta agcccgacgg tgcgcggcat tctggatccg gtaatcgaac tttatcgtcc
     2161 ggtgccgccg ctggcttatt tgccgctgat ggtgatctgg tttggtattg gtgaaacctc
     2221 gaagatctta ctgatctatt tagcgatttt tgcaccggtg gcgatgtcgg cgctggcggg
     2281 ggtgaaaagc gcgcagcagg ttcgcattcg tgccgcccag tcgctgggtg ccagccgtgc
     2341 gcaggtgctg tggtttgtca ttttgcccgg cgcgctgccg gaaatcctca ccggattacg
     2401 cattggtctg ggtgtgggct ggtctacgct ggtagcggcg gagctgattg ccgcgacgcg
     2461 cggtttagga tttatggttc agtcagcggg tgaatttctc gcgactgacg tggtgctggc
     2521 ggggatcgcg gtgattgcga ttatcgcctt tcttttagaa ctgggtttgc gcgcgttaca
     2581 gcgccgtctg acgccctggc atggagaagt acaatgagtg aacgtctgag cattaccccg
     2641 ctggggccgt atatcggcgc acaaatttcg ggtgccgacc tgacgcgccc gttaagcgat
     2701 aatcagtttg aacagcttta ccatgcggtg ctgcgccatc aggtggtgtt tctgcgcgat
     2761 caaactattg cgccgcagca gcaacgcgcg ctggcccagc gttttggcga actgcatatt
     2821 caccctgttt accctcatgc cgaaggggtt gacgagatca tcgtgctgga tacccataac
     2881 gataatccgc cggataacga caactggcat accgatgtga catttattga aacgccaccc
     2941 gcaggggcga ttctggcagc taaagagtta ccttcgaccg gcggtgatac gctctggacc
     3001 agcggtattg cggcctatga ggcgctctct gttcccttcc gccagctgct gagcgggctg
     3061 cgtgcggagc atgatttccg taaatcgttc ccggaataca aataccgcaa aaccgaggag
     3121 gaacatcaac gctggcgcga ggcggtcgcg aaaaatcctc cgttgctaca tccggtggtg
     3181 cgaacgcatc cggtgagcgg taaacaggcg ctgtttgtga atgaaggctt tactacgcga
     3241 attgtcgatg tcagcgagaa agagagcgaa gccttgttag gctttttatt cgcccatatc
     3301 actaaaccgg agtttcaggt gcgttggcgc tggcaaccga atgatattgc gatttgggat
     3361 aaccgcgtga ctcagcacta tgccaatgcc gattacctgc cacagcggcg gattatgcat
     3421 cgggcgacga tccttgggga taaaccgttt tatcgggcgg ggtgatacga gtgcggacgg
     3481 ttccctcgcc cccttgggga gagggttagg gtgagggggc gttcaccgta ctttcaacag
     3541 gttaactccc cctttctgag aggaaaaaaa ttaacgcaga atcttcttct cagccaaatc
     3601 cagcgcaaag tagctgaaaa tcagatccgc acccgcacgc ttaatcgaac ctaagctttc
     3661 gagcacgact ttctcttcat ctatagcacc cgccagcgcg gcgaacttaa tcatcgcgta
     3721 ctcaccgctc acctgatacg cgccaatcgg caattcagta cgttcacgca gctcacgcac
     3781 gatgtcgagg tatgctccag caggtttaac catcaggcag tctgcgccct gggcttcatc
     3841 cagcagtgat tcacgaatcg cctcgcgacg gttcattggg ttcatctgat agcttttgcg
     3901 gtcgcctttt aatgcgcttc cggcagcttc acggaacgga ccataaaagg aagaggcgaa
     3961 cttggtcgaa tacgacataa tcgccgtatc tttaaagcct gcagcgtcca gcgcctggcg
     4021 aatagcctgt acctggccat ccatcgcggc ggaaggggcg atgaagtctg cgcctgcagc
     4081 agctgcaacc acggcttgct tgcctaaatt ttccagagtt gcgtcgttgt cgacgccatg
     4141 ctcgcacagc acaccgcagt gaccgtgaga agtgtattca cagaaacaag tgtctgacat
     4201 gacgatcatt tctggcacag tctgcttgca gatgcgcgac atacgcgcca ccagtccatc
     4261 ttcccgccag gcgtcgctgc cggtttcatc ggtatggtga gagatgccaa aagtcattac
     4321 ggaacggata ccggcgttgg cgatgcgttc aatttcgcgt gccagatgtt tctctggaat
     4381 gcgcatcacg cctggcatgg cttcaacggc tttgtagtcg tcaatttctt cttcaacaaa
     4441 gatcggcaac accaggtcgt taaggctaag tgttgtctct tcaaacatag cgcgcagcgc
     4501 aggagatttg cgcaggcgac gagggcgttg gattaagtct gtcatggtct gcctgatgtt
     4561 tgtggaatta aggtgcacag tatacctgaa gcggggcagg agtgttttac gaaagttgtc
     4621 gtgatgttga caagaagaga atgaaagaga gaccaggaca tctttgtaat agaaatagca
     4681 ataatttcat attcactgaa aatattttta atctttattt atagctcttg gttattattt
     4741 tttggcg
//
LOCUS       DAZFRJ010000262         4728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000262 DAZFRJ010000000
VERSION     DAZFRJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.264"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..896
                     /locus_tag="ACHKUN_004436"
     CDS             <1..896
                     /locus_tag="ACHKUN_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO6662554.1"
                     /translation="QIFTTLRNIDLNLLTIFEAVYVHKGIVNAAKVLNLTPSAISQSI
                     QKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIA
                     TTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQ
                     HHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFSGLRQRVQEMFPDR
                     QINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHY
                     NKFSLRDPILHGVIDVIRNAF"
     gene            1105..2070
                     /gene="lipA"
                     /locus_tag="ACHKUN_004437"
     CDS             1105..2070
                     /gene="lipA"
                     /locus_tag="ACHKUN_004437"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO6662555.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2171..2374)
                     /gene="tatE"
                     /locus_tag="ACHKUN_004438"
     CDS             complement(2171..2374)
                     /gene="tatE"
                     /locus_tag="ACHKUN_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO6662556.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2503..3291)
                     /locus_tag="ACHKUN_004439"
     CDS             complement(2503..3291)
                     /locus_tag="ACHKUN_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO6662557.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3384..3767
                     /gene="crcB"
                     /locus_tag="ACHKUN_004440"
     CDS             3384..3767
                     /gene="crcB"
                     /locus_tag="ACHKUN_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO6662558.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3822..4031)
                     /gene="cspE"
                     /locus_tag="ACHKUN_004441"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3822..4031)
                     /gene="cspE"
                     /locus_tag="ACHKUN_004441"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6662559.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4206..>4728)
                     /gene="pagP"
                     /locus_tag="ACHKUN_004442"
     CDS             complement(4206..>4728)
                     /gene="pagP"
                     /locus_tag="ACHKUN_004442"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO6662560.1"
                     /translation="FIQLISVGKVFANADEWMTTFRENIAQTWQQPEHYDLYIPAITW
                     HARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWE
                     STWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYN
                     NGNVYFAWMRFQF"
BASE COUNT         1236 a         1207 c         1070 g         1215 t
ORIGIN      
        1 cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt tttgaagctg
       61 tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc ccctcggcaa
      121 tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt attcgcaaag
      181 gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc agtcagggcc
      241 ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa cgaacgataa
      301 ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat cgggcgatta
      361 aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg gaaaaccaac
      421 tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc
      481 aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcgaggga aatccactac
      541 tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt ctgttaccgg
      601 aagagcaaaa tttcagcggt ctgcggcaga gagttcaaga aatgtttccg gaccggcaaa
      661 tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac agtgacatgt
      721 tagcgattat tcccagccgt ttttataacc tgtttagccg ctgctggccg ctggaaaaac
      781 tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac aacaaattca
      841 gcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca ttttgaaaaa
      901 gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca acaacaattt
      961 tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata ctgccagtcg
     1021 ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct ttccttcgta
     1081 attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg cggtgttaaa
     1141 taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa
     1201 gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc tacacgtatc
     1261 cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc
     1321 tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat cctcggcgct
     1381 atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt agctcctgat
     1441 gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg ttatgtggtt
     1501 atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccagcactt tgcggattgc
     1561 attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt gccggatttc
     1621 cgtggtcgta tggatcgtgc tctggatatt ctgactgcaa cgccaccaga tgtgttcaac
     1681 cataacctgg aaaacgtacc gcgtatttac cgtcaggtac ggcctggtgc agattacaac
     1741 tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc gaccaagtct
     1801 ggtctgatgg tgggactggg tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg
     1861 cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag ccgccatcac
     1921 ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc cgaagcgctg
     1981 gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat
     2041 ttgcaggcga aagggatgga agttaagtaa tcctgtaata attacttaca tctgtctgat
     2101 aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca agatattgag ggagcgtcct
     2161 gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt
     2221 ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa
     2281 ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa
     2341 ctactagcag tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc
     2401 aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata
     2461 gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata attgcggcgg
     2521 cgcaaagcga cggttgttta agacgggcag ttgcgcgcgc acctgacgca cacgttcggg
     2581 cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa
     2641 gggatctata atccggcttt gaccgatatt tttgttcccg cactcccccg ccgccaccat
     2701 ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccaatgat gctctttgag
     2761 cggcccgcga acccaggcgg caggaagtac caggatttca gctccctgta atgcctgtgc
     2821 cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccctc
     2881 cacctcgagt aacggagcga tttcattacc agcatcaaca cggcgtgatt cctgaatggc
     2941 aaatgcatca tagagatgca atttggcata acgggcgacg atgtttcctg cctgtagcgc
     3001 caccagcata ttccatgcgc gccccggcgt tgaaggaaca tgaatcgtca gaattgtcgt
     3061 catcatgtta cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt ccagcagctg
     3121 tgctgattta accgatagat ctgcatcatg atcatcgcgc gccagcaatg cttccggcag
     3181 gacgaacagc gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac aaatctcagc
     3241 gttcttttcc cacacagatg taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc
     3301 ctgtcagagt cgtaaaattc gccaacgacg attattcggc gttacactta tcactcatac
     3361 aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt attggcggtg
     3421 gtacgggaag cgtggcgaga tggctgttaa gtatgcgatt taacccgctg catcaggcga
     3481 ttccgttggg gacgctggca gcaaacctga ttggggcatt catcatagga atgggattcg
     3541 cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc accaccggat
     3601 tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt ttgttacaag
     3661 agggccgctt tggctgggcg ttactgaacg ttttcgtcaa ccttctgggg tcttttgcca
     3721 tgacagcact ggctttctgg ctgttttcgg cctcaaccgc acactaaaga ccaaaaaaaa
     3781 acccgctgat taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt
     3841 gcagcagaag ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca
     3901 agagttttaa aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg
     3961 tcttccggag taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta
     4021 atcttagaca tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttacc
     4081 agtacaccaa ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg
     4141 cgttattttt gatgttactt attttagcta ttgattttaa agaagttact aaaacttcat
     4201 ttgtctcaaa actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc
     4261 ggaatgtagg tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga
     4321 accgggagag ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga
     4381 cctaaatgaa aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca
     4441 atcggttccc atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt
     4501 cctttttcat cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga
     4561 tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa
     4621 tcataatgtt caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatccac
     4681 tcatctgcgt tagcaaaaac tttaccaacg ctgattaact gaataaaa
//
LOCUS       DAZFRJ010000263         4721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000263 DAZFRJ010000000
VERSION     DAZFRJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.265"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..172)
                     /locus_tag="ACHKUN_004443"
     CDS             complement(<1..172)
                     /locus_tag="ACHKUN_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662561.1"
                     /translation="MLKQRDMTETARAVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQF"
     gene            complement(385..1074)
                     /locus_tag="ACHKUN_004444"
     CDS             complement(385..1074)
                     /locus_tag="ACHKUN_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6662562.1"
                     /translation="MNNQYLQFVREQLMIATADLSGATKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFATSSWRRTLLELEEHQK
                     AWLLWCYGGNICWEHQIAITQWVWNEFKTQSGTRKIAVKTLERVKKLIWLAAQDVRGW
                     VTGCEVYQRQELARLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKA
                     TFSRRDIAKVN"
     gene            complement(1071..1430)
                     /locus_tag="ACHKUN_004445"
     CDS             complement(1071..1430)
                     /locus_tag="ACHKUN_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6662563.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLEIKITEVGCA"
     gene            complement(1443..2492)
                     /locus_tag="ACHKUN_004446"
     CDS             complement(1443..2492)
                     /locus_tag="ACHKUN_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6662564.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWLHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLREQTSDSLDQLAQQNLAAWMIDIIRHAMNGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPSHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(2494..2772)
                     /locus_tag="ACHKUN_004447"
     CDS             complement(2494..2772)
                     /locus_tag="ACHKUN_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662565.1"
                     /translation="MAHDTKLHNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGFEVKKALSLDYRHWIISVRPPYSERPPRPSRTFQQRIWR"
     gene            complement(2940..3095)
                     /gene="hokD"
                     /locus_tag="ACHKUN_004448"
     CDS             complement(2940..3095)
                     /gene="hokD"
                     /locus_tag="ACHKUN_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6662566.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(3342..3446)
                     /locus_tag="ACHKUN_004449"
     CDS             complement(3342..3446)
                     /locus_tag="ACHKUN_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662567.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(3562..4275)
                     /locus_tag="ACHKUN_004450"
     CDS             complement(3562..4275)
                     /locus_tag="ACHKUN_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6662568.1"
                     /translation="MHRVCLEANRYLDKYDAMAKEVNKLLGRIAPPAPVFNGEYGDAY
                     QGAREDLSIWKRRALEADEHVRRLEQINDHMVKEAQGESRMGEPVIREPAPVVPEEAT
                     PENVEMLSGYVSTYKLTDSERDIAAEIWNACRAAMLQAGNFRENKGSSTNNFREISET
                     STNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGEFFDVVRNGDEFFETQ
                     SRNVTDWMPLPEPPQEVRQ"
     gene            complement(4476..4688)
                     /locus_tag="ACHKUN_004451"
     CDS             complement(4476..4688)
                     /locus_tag="ACHKUN_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662569.1"
                     /translation="MSNAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPE
                     GVDPNYCWWDVDPVDAANEAMSYHND"
BASE COUNT         1098 a         1276 c         1070 g         1277 t
ORIGIN      
        1 caaactgata gtctgccaga cccgccataa caagctgggt cagtattagc tggcagcgtt
       61 cgcgtgaaag gtacgtattc tgtgcaatct ccccgactgt cgccggttcg gtggcactta
      121 attcattaaa aactgctctg gcggtttctg tcatatcccg ctgtttcagc atgtcttttt
      181 ccctttttcg gttaacgtga cacaccaata actcttgtcg aaaaagccag caagctgaaa
      241 gaccggtatt caccgccgcc agcgcgttta ctgtactgac gcgattttcg gacataaaaa
      301 aaccgcctgg cggcagtttt tttcttattt tgtcatcgcg tacaaaatag gcaaaatatc
      361 agatttatac gaaacatacg cgatttaatt gacttttgca atatctcgtc gtgaaaaggt
      421 cgctttttgt tgcgctctta ttttcacgga gcaaatcaag gattctctat cgaggcgctt
      481 aaaaatatcg cacatctcac gccagtagtt cgcataatta tggctccagt tatcaggctt
      541 aactccacac agtctggcaa gctcctgtct ctggtagacc tcacacccgg taacccatcc
      601 tctgacatcc tgtgccgcca gccagatcaa cttcttcaca cgctccagcg ttttcactgc
      661 aatttttctg gtaccagact gagttttaaa ttcattccac acccactgcg ttatcgcgat
      721 ctgatgctcc cagcaaatgt ttccgccata acaccacaac aaccacgcct tctgatgttc
      781 ttccagttcc agaagggtac gccgccacga tgatgttgca aactcaacag gactgaccag
      841 ggcaattgat gagcctttcg ccagtgattg tttacccggg attgggggat tatcccgcgt
      901 gatcattttt ccggttacct catcgcggta acgaattttt ttgcgtctgt aacgccctgt
      961 atcgaacagg gcattttcct gccaggcttc cagttgacct tttgtcgccc cactgagatc
     1021 cgcagtggca atcatgagtt gctcacgaac aaactgtaaa tactggttat tcatgcacac
     1081 cccacctctg taatttttat ctccagccgt ccaccagata ctggcttgcc acgtacaata
     1141 ttgatttcat caaactgctc atcgtccatt aacaaccccg cgtgcgtcag cgcatccagc
     1201 ggtgctttca gaatattgtc caggtcacgg cggcgcttat ccggtggctc tgcaataatt
     1261 tttattgcca gccgtccgga caggcttaat ttcagtcgct gctggcgaac aataagcgcc
     1321 actgcccggc gataacgctc cccggctttt gatacaaaat atgtgctgcc acggcgtcgc
     1381 cagtaagtgt tcaccgtcgg cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc
     1441 ttttacccaa gcacgccagt tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc
     1501 tgagaaccat gcttttcttc gaacgccagc ggatccgcat gaagctcgtt gtgatgctcc
     1561 cgacacagcg gtagcgtgaa aatatcgtga gattttgtcc ccattccgcc ctgaccatga
     1621 ccaatcaggt gatggggatc gtcggctggc ttaccacaac acgcacacgg ctgtgtcttt
     1681 acccagcgtg tgtatttctc atttacccag cggcgacgtt taggtcgttt catgaaggat
     1741 tccggagact ccggatcaac ggcaatgctg accaccgtct tttcctgtgg tgggttctgt
     1801 tgctggtggg tgtgagacgg tagcgcaata ttttttgtgc gctgcttcag tatgctggtg
     1861 gcggtctgtt ctcccggtat gatgtcgctt tcacggtata cggagcggat tttttccgca
     1921 cgcaacccca gcgaacgacg taataccgct tccggtagcg cgtccgccac ctgattgcgg
     1981 accgcccacc aggataattc agccagagat aattcacgct cctgtgcgcc attcattgcg
     2041 tgacggatga tgtcaatcat ccaggcggcc agattctgtt gagcaagttg atccagtgaa
     2101 tcagatgtct gctcccgcag ctggttgtcg cagtgccagc acaacaccat cgcgccggta
     2161 ccgtaacggt gaatgacggt ttcgctgtga tgataatcac cgtgtagcca ctggcaggat
     2221 ttcacgtgac gtaataacca gtcagacagt gcaccagcgc cacccgcagc acggatcacc
     2281 cgctcatcgc tgaaaaatgg cagtagtgat ttatcttccg ccagcggctg gtgaacggca
     2341 ggtacaaccc cggacggcag agctcgcatg ctttttggtt ccggctccac cagtattctg
     2401 ccgttatgga atgctgacat tgattcacgg cctggcttaa cgataaccag accgagttcc
     2461 ggtaccagaa caggtcgaag taatacccgc acattacctc cagatccgtt gctggaatgt
     2521 gcgggacgga cgcggtggcc gttcggagta agggggcctg acggagatta tccagtgacg
     2581 gtagtcgagg ctaagggctt ttttaacctc gaatccgcgc ctgcggtaac actgaatgag
     2641 ccattcggcc tgttcttcag tgcatggggg atgctggtac cagtcagatt tgaatgcgtg
     2701 aaaacaccgt ccgcgcctgc tggcaaagac ggcagaatca tcagaattgt gtaatttggt
     2761 atcgtgcgcc atcggctgtc tctgctggcg cagcaggtgc cagttgttca ggctggcgtg
     2821 cgaattgtaa accagaatgc caggaaaaaa caaaacccgc cgaaacgggt taagtgcggg
     2881 tgcgttgagg atgcctgaca catcagaggt ggcgagggat ttctcccccg cctggtctct
     2941 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggattcgt
     3001 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
     3061 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gcctttcggc
     3121 acgtaagagg ctaacctacg tttgtgaagc atagattggg cctcagatta atgttaaacg
     3181 tcttgcagga cgcgtaatgt taactggggc ttttctctat ctgccttttg gtgttcatgc
     3241 ctgagacaga tagcctcaag cacccgcagc aattctactt aactctcgct ttaccgcaaa
     3301 ccgtttttac ccgatatggg aattcccata tcgtaatgaa ttcagttccc tagtcgatcc
     3361 atcaaaaaca caaccaggca gtaaacgccc acaacagcaa taacagccag cgcaccttcc
     3421 attgccagtg aaatatcatc cgacatattc cctcctttgg tgtgaatccc ggcgaacgtt
     3481 tttaccccca ccgacaaata acatatacta aaaaatcaat agctatagca acgcctgtaa
     3541 ttgcaaaggc ttcaggccag atcattggcg cacctcctgc ggcggttctg gtagcggcat
     3601 ccagtcggtt acattgcggc tctgtgtttc gaaaaattca tcaccattac ggactacatc
     3661 aaaaaactca ccgtctcgat attgcgcata aagaacgaat gcgccatcac ataaaataat
     3721 tacgtgctga ccatcatccg gcattcgctc actacagctt atccaaccat ccggagttac
     3781 cggatagttg gttgacgttt ccgagatttc ccgaaaatta ttggttgacg aacccttatt
     3841 ttcccgaaag tttccagcct gaagcatggc ggcgcggcag gcgttccata tttcggcagc
     3901 aatatcgcgc tcgctatcgg ttaatttgta cgttgaaaca tagccagaga gcatttctac
     3961 gttttccgga gttgcttctt caggcactac cggtgctggc tcacgtatta caggctcgcc
     4021 catgcgtgat tctccctgcg cctctttcac catgtggtca ttgatttgct ccagtcgccg
     4081 aacgtgctca tcagcttcaa gcgctcgccg tttccagatg gacaggtctt cacgagcgcc
     4141 ctgatatgcg tcaccgtatt cgccgttaaa tactggcgct ggcggggcga tgcgtccaag
     4201 caacttattt acctctttcg ccatcgcgtc atatttatct aaatagcgat tagcttctaa
     4261 gcagactcgg tgcatctgat ctgagttaac tcgtttaact ggatctgctt ccagctatgc
     4321 cagtgcaatt ttgaataact cgccctatac ccgtgccatc cctgaattgg ggtagcattt
     4381 cgcaatcgct atttttaatt tggcttcttc gattaatttc tctttggtta attcagtcac
     4441 ttttcattac cgccctttcg ggcggcctcc tgacattaat cgttgtgata actcatagct
     4501 tcatttgcag catcaactgg atcaacatcc caccagcaat aatttgggtc gacaccttca
     4561 ggtgtccacg gctctaattc attttttgcc gcattctcgt cgccagtaat tttaaaaatc
     4621 tgctctgaga actttttcac ccactcgtta tatttttcag cgttaataac tttctgtgcg
     4681 tttgacattg atatacctcc ggttaaggat taaattttta a
//
LOCUS       DAZFRJ010000264         4720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000264 DAZFRJ010000000
VERSION     DAZFRJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.266"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..145)
                     /locus_tag="ACHKUN_004452"
     CDS             complement(<1..145)
                     /locus_tag="ACHKUN_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6662570.1"
                     /translation="MFSANSSATPALRGNEVSMDTIDLGNSESLVCGVFPNQDGTFTA
                     MTYT"
     gene            complement(284..691)
                     /locus_tag="ACHKUN_004453"
     CDS             complement(284..691)
                     /locus_tag="ACHKUN_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662571.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            769..996
                     /locus_tag="ACHKUN_004454"
     CDS             769..996
                     /locus_tag="ACHKUN_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6662572.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            980..1501
                     /locus_tag="ACHKUN_004455"
     CDS             980..1501
                     /locus_tag="ACHKUN_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6662573.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            1482..2447
                     /locus_tag="ACHKUN_004456"
     CDS             1482..2447
                     /locus_tag="ACHKUN_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662574.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            2488..2907
                     /locus_tag="ACHKUN_004457"
     CDS             2488..2907
                     /locus_tag="ACHKUN_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6662575.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            2962..4059
                     /locus_tag="ACHKUN_004458"
                     /pseudo
     CDS             2962..4059
                     /locus_tag="ACHKUN_004458"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            4132..4281
                     /gene="ydfW"
                     /locus_tag="ACHKUN_004459"
     CDS             4132..4281
                     /gene="ydfW"
                     /locus_tag="ACHKUN_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO6662576.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
BASE COUNT         1313 a          988 c         1226 g         1193 t
ORIGIN      
        1 tggtatacgt catcgcggtg aacgtgccgt cctggttggg gaacacgcca cataccagag
       61 attcgctgtt gccaagatcg atagtatcca tgctgacctc atttcccctt aacgccgggg
      121 tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tggaggtaat ttagttttct
      181 cattattttt cgtcaaggtt ttttgatgag aaaactcaag cgttggcgca agataaagcc
      241 aatacattga aatgtaaggc tttaaaattt tgtgaaggga tgattattga tgcttgttgc
      301 gtttgcgagc ctctaatagc tcggtgaaca ggcgattaaa attctcaacg cgggcgcgga
      361 gttcgctgag ttgtgcttgt tgctctgatt taggaagtgc gcgatacaaa cgcagcatct
      421 ctaactcatc ttccgataag tctaaggcac tatcgagtga aacaggggga gctggtgttt
      481 tgtcctcgtc gccaaacagt atccaagttg gtgaacattg caatacctcg gcgaggcgat
      541 gcaaattttg cccgcgcggg gctgtatggt cactttccca taatgaaatt gatgagccag
      601 atacgccagc agctttgctt aaaccgtttt gacttaagcc tacctgctta cgtctttctc
      661 taatgcgttg acctaaagtt ttctcgttca tatttagata tcttaataac ccttgacttg
      721 agattccttg agtgattagc attgagaaaa ctcaatattg gaggtgcgat gtttaaatca
      781 gacgtaatta atttttacgg tacgaaagcc aaagtagcga aagctgctgg cgttgaccca
      841 tctgctgttt ctcaatggca agagctggtt cctgaaggtc gcgcgatgcg tctacaggag
      901 gcatctggcg gcgagcttct gtatgatccc aaggtttatg acgaatatcg taagacgaag
      961 cgggcggggc ggttgaacaa tgaaaatcac tcctgaacag gctcgtgagg ctctggatgc
     1021 ctggatatgt cgaccaggaa tgacacagga gcaggcgacg atattaatca ctgaagcatt
     1081 ctgggctttg aaagagcgcc cgaacatcga tgttcagcgt gtcacagatg aaggtggcgc
     1141 ggttgatcag cgagcacttg gcgttaatcg agtgaagata ttcgaacgct ggaaggctat
     1201 cgacaccagg gataagcgtg aaaagttcac ggcgctagtg cctgcaatta tggaggctat
     1261 tcggattaat gatttcaggt tgtatcgtga aattagtgac ggaaaaagca tcacgtacat
     1321 gatcgccggg ttaaacaaag aatatggcga tgtggtggag tccggactgc tttttgctga
     1381 tcctgccgta gtggatcgtg aaactgacga acttatagaa aaagcaattg ctttcaaact
     1441 tgcgtatcga cagcaatacc aacaaaaagc tggatggaat tatgagcctt ctttttgctg
     1501 aacgcccact ggttataaac acacagcttg cgatgaagat tggcttaaat gaagccattg
     1561 tgttgcagca gttgcattac tggttgagag ataccaattc cggcatggaa tgtgatggtg
     1621 ttcgctggat ttacaacaca acggaacaat ggctggaaca gttcccattc tggtcagagt
     1681 caacgttaaa gcgcgcgttt gcaagtctga aaacgctggg gcttttgcgt tgtgaaaagc
     1741 tcaataaatc aaagcgcgat atgaccaatt tctacacgat caactacggg agcgagcttt
     1801 tagatggtgg caaattgagc gaatccatcg gtttaaaatg cgccgctcca tcaggtcaaa
     1861 atgacacgat ggaagaggtc aaaatgaaac gctccattgg ttcaaaacga cccaatgtca
     1921 tcgggtcaaa atggcctgat gatcttacag agaatacaac agagattact acagagaata
     1981 aaaacacttt tcgtccggaa gcttcgcaac cggaccagca gaccgctgaa caggattttt
     2041 taacccggaa ccccgacgcg gttgtgttta gtgcgaaaaa acgccagtgg ggtagcaggg
     2101 aggatttggc gtgtgcgcag tggatttggg ggcggatcgt gaacctttac gaacaggctg
     2161 ccagcgacga tggagagatc atgcgaccaa aagagcctaa ctggacagcc tgggccaatg
     2221 acgtgcgcac aatgcggatg ctggatggca gaagccacag acaaatttgc gaaatgtttg
     2281 gtcgggtaca gcgagatcaa ttctgggtaa aaaacatcat gagcccgtca aaactccgcg
     2341 aaaaatggga cgaactggtc atccgcctgg gacgttcacc tgtacagcgt tgtgtgaatc
     2401 atatttctga accggataca gaaattccgc ctggtttcag gggataagtg ttgattttag
     2461 ggtataaagt aatgttcagg aggacttgtg gcaaaagttt ttacacaaga agagcgggaa
     2521 aaaattaaag ggcaggtggt ggaactcgta cgccagagtg ggcgcgagac gttacgacaa
     2581 ctggaagcta aaactggggc aacaagatat ctgatgagcg ttctggccag agagctggtt
     2641 gccagtggcg atgtatacag ctctggttac gggttattcc cgtctgaaca ggcgcgtaag
     2701 gactggcaaa acgcccgcaa aaaactctcg agggcaaagg tgaagaaacc tgctgtggtt
     2761 gatccggacc ttatctggtc attacctgac ggagaaatac gtcgctacga cagtcgtcta
     2821 aacataatct gtcgcgagtg ccggaaggca aggagaagca ggctattgtt aacaccaagc
     2881 tgtaatgtcc cctttgaacc attctaaaat gtccccagac aattctctgg gggatttttc
     2941 atgatcaaag agactgttac gatgagtcat aaggaactcc accgacttca gattattcag
     3001 gagtcaatta atcgacatat tactcaggaa caagctgcgg cacgcattgg catttctatt
     3061 cggcaggtta aacgtctggt gcaacggtat agaaatgaag ggccttctgg tctggtttcc
     3121 caccgacgtg gaaagcgtcc taataattcc ttttctactg aattcagagc aacagtaatt
     3181 tcactcctca aaggccgtta cgctgatttt ggacctacgt ttgcgtgcga aaaattgcgc
     3241 gagatacacg gtttatcttt atccgttgaa actctcagaa agtggatgat agaagagggg
     3301 ttatggcgtg aacgccgtcg taaaattgcc cgtatatatc aacgccgcat gcgacgacca
     3361 tcttacggtg aactgatcca gattgatggc tcacctcatg actggtttga aaatcgaggc
     3421 cccagatgta cactgatcgt tttcattgat gatgccacca gtgcgttgat ggcgttgcgt
     3481 tttgtgcctg ctgaaacaac ccgggcttac atggaaaccc tccggggtta ccttaatgat
     3541 catggcgtac cgctcgctct ctactctgat agacacagta tattcagggt aaataaccca
     3601 gagcgggaag gtgagctgac ccagttcact cgtgcgataa agacactggg catcgagcca
     3661 atccatgcca acagcccgca ggcaaaaggg cgggtagagc gcgccaatca gacactacag
     3721 gacaggctgg tcaaagaaat gcggcttcag aatatcagtg atattgaaac agcaaatgca
     3781 tggttgccga cctttattga agcctataac aaccggttcg ctacgtcgcc tcgtactact
     3841 gataatgctc atcttgatgt gcaccattct gaagaggaac tgggttatat cttcagccta
     3901 caggcgaagc gcgttctgtc taaaaatctc actttccagt acaaaagcag tgcgtttcag
     3961 gtacgcagtg agggccgggg atatcgactt aggcattcgg ttgttactgt atgtgagaac
     4021 tttgacggtg aaattaacgt tctgtatgac gggaaagcgc tgggctggga aaagtatgtt
     4081 gatggcccgg agcctatacc actggatgat gaaaagagtg tccatgaacg agtggataat
     4141 gcccgtattg atttacgctc aaaatactat gttaaatcta aagctgacca tccctggctt
     4201 acgcgccgaa cgcaaagtca tcagcaagtt aagcccccga agttacctaa aaagaagcct
     4261 gatcccgata aaatagattg aaaccaagat cgattcggtt gagtgcatat ccattcatag
     4321 ggtagattct taagtcgcgt ttctgatgtt cattttcggg tggtttgtta cttgttttac
     4381 cggggatatg ccagaaacgc gctgagtcag tctgggcggt gcgcgtaatg aggcgttatg
     4441 gtaaatagcc tatgctaatg tccgctaaga gcaagaagcg gaagttggca gttttgtgga
     4501 ctgtccccac aaaagtgact acagaaatag ttgcaattca taattgatca tgggttgtca
     4561 gttaaactcg tggcgattta aatagactaa ttgggagtgc gtccattact tatatcttgt
     4621 aatgttaact atcagaaatg atacaaagat aatatgtctt taaagaaaag gctgatggcg
     4681 aaaagtggcc cgatgagggc cacaatacgg ctgtcactta
//
LOCUS       DAZFRJ010000265         4638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000265 DAZFRJ010000000
VERSION     DAZFRJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.267"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1050
                     /gene="tsuA"
                     /locus_tag="ACHKUN_004460"
     CDS             <1..1050
                     /gene="tsuA"
                     /locus_tag="ACHKUN_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO6662577.1"
                     /translation="MILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLI
                     AISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEG
                     LIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVLLVI
                     TLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEAT
                     GRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADA
                     QTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVY
                     VRPQRKARLATAAAN"
     gene            1064..1291
                     /gene="tsuB"
                     /locus_tag="ACHKUN_004461"
     CDS             1064..1291
                     /gene="tsuB"
                     /locus_tag="ACHKUN_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO6662578.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1334..2761)
                     /gene="sbcB"
                     /locus_tag="ACHKUN_004462"
     CDS             complement(1334..2761)
                     /gene="sbcB"
                     /locus_tag="ACHKUN_004462"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO6662579.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            2970..4136
                     /gene="dacD"
                     /locus_tag="ACHKUN_004463"
     CDS             2970..4136
                     /gene="dacD"
                     /locus_tag="ACHKUN_004463"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO6662580.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4254..>4638
                     /gene="sbmC"
                     /locus_tag="ACHKUN_004464"
     CDS             4254..>4638
                     /gene="sbmC"
                     /locus_tag="ACHKUN_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO6662581.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPK"
BASE COUNT         1123 a         1050 c         1225 g         1240 t
ORIGIN      
        1 atgatattaa gcgggctaat ttgtggtgct ctgctgggat ttgtcatgca gcgtgggcgt
       61 ttttgcctga caggtggttt tcgcgatatg tatatcgcga aaaataatcg catgttttac
      121 gccttgctga ttgcaatttc ggtacaaagc gtgggggttt ttgcgttaat tcaggcgggc
      181 ctactgactt acgaagccgg ggcgttcccg tggcttggta ctgttatagg tgggtatctt
      241 ttcgggctgg gaattgttct ggcgggtgga tgtgccaccg ggacctggta tcgcgcgggt
      301 gagggattga tcggcagttg gatcgcgctt ttcacttata tggtgatgag tgcggtgatg
      361 cgttctccac acgccagtgg tttaaatcaa accttgcagc actacactac tgaacataac
      421 tctattgctg atacttttaa tttgtctgtg tggccgttgg ttgccgtttt gctggtgata
      481 acgctctggg tggtgatgaa agagttgaag aagccaaaac tcaaagtcgc gaccttaccg
      541 ccgcgccgaa ccgggatcgc tcatattctg tttgaaaaac gctggcatcc ctttgtcact
      601 gctgtactca tcggtttgat tgcgctttta gcttggcccc tgagtgaagc aaccggacgc
      661 atgtttgggt taggaatcac ttccccaacg gccaatattc tgcaatttct ggtcgcgggt
      721 gacgtgaaat acattaactg gggcgttttc ctggtgttag ggatcttcgt ggggtcattt
      781 attgcagcca aagcgagccg tgagttccgc gttcgcgctg ctgatgcaca aacaacatta
      841 cgtagcgggt taggtggtgt actgatgggc ttcggtgcca gcattgcagg tggttgctct
      901 atcggtaatg gactggtcat gactgcaatg atgacctggc agggctggat tggccttgta
      961 tttatgattc tcggagtctg gactgcgtcc tggcttgtgt atgttcgacc gcagcgtaag
     1021 gcacgactgg caactgctgc ggcaaattaa gtaaggtgta aagatggcga ttaaaaagct
     1081 ggatgtggtg acgcaggtct gtccgtttcc actcatagaa gcaaaagcag cactggcaga
     1141 aatggccagc ggcgatgagt tggtaattga gtttgattgc acccaggcaa ctgaagcaat
     1201 cccacagtgg gcggcagaag aaggacatgc catcaccgat tatcagcaga ttggcgatgc
     1261 agcgtggagc atcaccgttc aaaaagcctg actcaaaatt gtcctccgcc gtaccagcgg
     1321 cggaggcttc acattagaca atctcttccg cgtactgcca aagcgctttt aacagcgcca
     1381 ctttttcttt atcgtcggca tactgctgcg ccagcatctg aatctcttct gcatagcctt
     1441 gtaaaaactc tggcgtgaag acctggcggc ggtgctccag ccagcgttgc tgctcggcat
     1501 aatccagcgt ccccgggaag ttgcgcgccc gataattgaa caacagcttt tcaattcgtt
     1561 tatcaacaaa agtgatatcc agtgccggta aattacgcgg ctcggtttcc agcacaattt
     1621 tcattgctgc acgatcggcg tcactgaaaa agccgttata aagctgtgca tccacattat
     1681 ctgaaggtgt aaacggttcg gcttccacga atatcgctac cactttttca cgcacttgcg
     1741 gattttcacg cagaattttt aggttatcga ggcaatgctg acgattaatt cccagtcggt
     1801 cggcatcttc cgggcgtagc gtattcgcct gggccagtac cggacattta ttgatatgta
     1861 ccagcttaac cggaacggcg gcgttatcgc caagatcggc ttttgcggta tataaacgct
     1921 cgcgcaatgt gtcgctatcc agctccagta atggcgaaat gtctcctgcc aaatccacca
     1981 taattacggc attgcgattt tcaggatgcc acgccagcgg tgccacccag cttgtattgc
     2041 cgcgccatgc accaaacatt ccggaaacgt gaaccagggg tttcatctga ggaacatcaa
     2101 tcaacgccat tagtttgtgt ttattacgat gggtaaagag ataatcaaac aggcgtggct
     2161 gacgcgtttt taccagtttt gccatcgcaa tagtggcgta cacatcagcc atcgcatcgt
     2221 gggcgttgct atgttcaata ccattcgctt tggttaaatg ctcaaggcga aagctcggta
     2281 gaccgtcatc attttcaggc caatttattc cttccgggcg cagggcataa caggcacgca
     2341 taacatccag taaatcccag cgcgagttat catgctgcca gctccaggcg taaggatcgt
     2401 agaaattacg ataaaaaacg ttgcgtgtga cttcgtcgtc gaaacgcaca ttgttgtagc
     2461 ccagaataca ggtcttcggt acggtaaaaa gcgagtgaat acgggcggca aacgcggctt
     2521 cgttttctcc tttcgctcgt gtctcctgcg gggtaatacc ggtaattaat actgctccag
     2581 gctggggtaa atagtcatcc gcgggcttgc agtaaaagac ttcgggttcg ccgatgacat
     2641 tgaattcgtc atcggtgcga atggctgcga actgtgcagg gcgatctaac gcggggtgcg
     2701 tgccaaaggt ttcgtaatcg tgaaacaaaa aggtagattg ttgcttaccg tcattcatca
     2761 ttaggttaaa tccgttattt ctgctgtctg ccagagtatc aaatatcacc gtgctaatca
     2821 gctttagcgc gacaatttga cagcgagtga caacagatca tgtcagataa aaatgagagg
     2881 gtagtcacat tttcttgcac tttattccag ccagttcata agtatttccg taaaaagaac
     2941 agctatttga aactcctgag ggtttgctgt tgaaacgccg tcttattatt gctgcttctt
     3001 tgttcatttt taacttatcg tctggttttg cggcggaaaa cattcctttt tcacctcagc
     3061 ctccagagat tcatgccggc tcctgggtac tgatggatta caccaccggt cagatcctca
     3121 ccgcgggtaa tgagcatcaa cagcgcaatc ccgccagcct gacaaagctg atgacgggct
     3181 atgtcgtgga tcgcactatc gatagtcatc gcattacgcc agacgatatt gtcaccgtgg
     3241 ggcgcgatgc gtgggcgaaa gataatccag tgtttgtcgg ttcttcactg atgtttttga
     3301 aagagggcga tcgcgtatcg gtacgtgatt taagccgtgg tttaattgtg gattccggaa
     3361 atgacgcttg tgttgcactg gctgactata ttgccggtgg gcaacggcag tttgttgaaa
     3421 tgatgaacaa ctatgccgag aagctgcatc tcaaggatac gcattttgaa acagtgcatg
     3481 gtctggatgc accaggtcag catagctcgg cttatgattt agctgtgctt tctcgcgcta
     3541 tcatccacga cgagcccgag ttttatcata tgtacagtga gaaaagtctc acctggaacg
     3601 gtatcaccca gcaaaaccgt aacgggttat tgtgggataa aaccatgaat gttgacggcc
     3661 tgaaaacggg tcatacttct ggtgccgggt ttaatctcat tgcttcggct gtcgatgggc
     3721 agcgtcgtct cattgcagtg gtaatggggg ctgacagtgc aaaaggtcgt gaggaagagg
     3781 caagaaaatt actgcgttgg gggcaacaaa actttactac ggtgcaaatt ttgcaccgtg
     3841 ggaaaaaggt cggtacggaa cgcatctggt atggcgataa agaaaatatc gacctgggaa
     3901 cggaacaaga gttctggatg gtgctaccga aagccgaaat tccacatatc aaagccaaat
     3961 atacccttga tggtaaagag ctcaccgcgc caattagcgc ccatcagcgg gtaggggaaa
     4021 ttgaacttta cgaccgtgat aaacaggtgg cgcactggcc gctggttacc ctggaatctg
     4081 tcggggaagg cagcatgttt tctcgcctga gtgattattt ccaccataag gcctgacctt
     4141 tcttttgcag cagactggca ggagtgcgag tctgctcgca taatcaacac tcattccttg
     4201 tggttttaat atcgcaatta tactgtatat aaaaacagtg ttaatgggac gtcatgaact
     4261 acgagattaa gcaggaagat aaacgtaccg ttgcaggttt ccatctcgtt ggcccgtggg
     4321 aacagacggt aaagaaaggc tttgagcagt tgatgatgtg ggtagatagc aaaaatattg
     4381 tgccgaagga gtgggttgct gtcttttacg acaatccaga tgaaacaccc gccgaaaaat
     4441 tacgctgcga catcgtcgtg acggtgccga ataactttac gctccccgaa aacagtgagg
     4501 gcgtcattct gacagaaatt tcaggtggtc agtatgcggt tgcggtggct cgtgtagtcg
     4561 gtgatgattt tgctaaaccc tggtatcagt tctttaatag cctcttgcag gacagtgctt
     4621 atgaaatgtt accaaagc
//
LOCUS       DAZFRJ010000266         4593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000266 DAZFRJ010000000
VERSION     DAZFRJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.268"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..2565
                     /gene="hsdR"
                     /locus_tag="ACHKUN_004465"
     CDS             133..2565
                     /gene="hsdR"
                     /locus_tag="ACHKUN_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO6662582.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2632..4101
                     /locus_tag="ACHKUN_004466"
     CDS             2632..4101
                     /locus_tag="ACHKUN_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6662583.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4101..>4593
                     /locus_tag="ACHKUN_004467"
     CDS             4101..>4593
                     /locus_tag="ACHKUN_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6662584.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLAD"
BASE COUNT         1334 a         1114 c         1133 g         1012 t
ORIGIN      
        1 ttatcctact attgatgaaa ctcaagaata cctttaaaac agtgacttaa cgctgataat
       61 actctttgaa cagatgaatt catctatttt tctgtgtaga atttgcgcat cgcatttaga
      121 tgagaacacc ccatggcgga actgaaccta agtaacctga cggaagcaga catcattacc
      181 aaatgcgtta tgccagccat tctcaatgca ggctgggaca acacaacgca aatcagacag
      241 gaggtcaaac tccgggacgg taaagtcatc gtacgtggta aagttgcggc acgcagaacg
      301 gtaaaatctg ctgacatcgt gctgtatcac aaaccaggca ttcccttagc cgtgattgaa
      361 gcaaaagcca acaaacatga aattggcaaa gggatgcaac agggcattga atatgcgcgc
      421 ctgctggacg ttccttttgt ttttgccacc aacggggatg gctttatctt ccgcgatgcc
      481 accgcagccg aaggtgaatg ccttgaaaag caaataacgc tggatgactt cccctccccc
      541 gctgaactct ggcaaaaatt ctgcctctgg aaaggataca cacaagctca gcttccggtg
      601 attactcagg actattacga cgatggcagc ggtaaatcgc cacgttatta ccagcttcag
      661 gcaatcaaca aaaccattga agccgtctcc aacgggcaaa accgcgttct gctggtcatg
      721 gcgaccggaa cagggaaaac ctataccgca ttccagatca tctggcgcct gtggaaatca
      781 aaaaataaaa aacgcatttt gttccttgcc gatcgcaaca ttctggtcga ccaaaccaaa
      841 aataatgatt tccagccatt tggtacggca atgaccaaag tcagcggacg caccattgat
      901 cccgcttatg aaattcacct cgcgctctat caggctataa ctggcccgga ggaagaccaa
      961 aaagcgttta aacaagtcgc accagatttc ttcgatctga tcgtgatcga cgaatgccat
     1021 cgcggcagcg catccgaaga cagcgcctgg cgagagatcc ttgattactt cagttccgcc
     1081 acccaaattg gcttaaccgc cacgccaaaa gagacgcatg aagtctccag cacggattac
     1141 ttcggcgatc cggtttacgt ctactcgcta aaagaaggga tcgaagacgg cttcctcgcc
     1201 ccttataaag ttgtccgtgt tgatattgat gttgatctgc aaggctggcg cccaaccaaa
     1261 gggcaaactg acttaaacgg cgaagtgatc gacgatcgta tctataacca gaaagatttc
     1321 gatcgcacga tggtaatcga cgaacggaca gaactggttg ccagaaccat taccgactac
     1381 ctcaagcgca ccaacccgat ggataaaacc atcgtcttct gtaacgacat cgatcatgca
     1441 gaacgtatgc gccgcgccct ggtgaatctc aacccggagc aggtgaaaaa gaacgacaaa
     1501 tacgtcatga aaatcaccgg cgatgatgaa attggcaaag ctcagttgga taacttcatc
     1561 aacccgaaaa aagcgtaccc ggttatcgcg accacttcag agctgatgac cactggtgtg
     1621 gatgctaaaa cctgcaaact ggtagtactg gaccagaaca tccagtcgat gaccaaattc
     1681 aagcaaatta tcggtcgtgg tacacgcatc gacgaacgtt acggcaaact ctggtttacc
     1741 atcctcgact ttaaaaaagc caccgaactg tttgccgatg agcgtttcga tggcattccc
     1801 gaaaaagtca tggataccac accagaggat atcgccgatc cagaatctga ttttgaagag
     1861 aaactcgaag aaatcagcga acatgacgac gaacaggtaa caggcgttga tgaaccgcct
     1921 gcgccaccat accaggttac cgataccgat gatgtcggcc cacttccgga agaagacgag
     1981 aagaaaatcc gcaagtttca cgtcaacggt gtagcggtgg gcgttattgc gcagcgtgtt
     2041 cagtattacg atgccgacgg taaactggtt accgaatctt ttaaagatta cacccgcaaa
     2101 acactgctca aagaatatgc ctcgctggat gactttaccc gcaagtggca ggacgccgat
     2161 cgcaaagaag cgatcattca cgagctggag caacagggga tcatctggga agtactggca
     2221 gaagaagtcg gtaaagatct cgacccgttc gacatgcttt gccacgtagt gtatggtcag
     2281 ccgccgttaa cccgcaaaga gcgcgccgag aacgtgcgca agcggaacta cttcacaaaa
     2341 tactctgaag cagcgcaagc cgtgctcgat aatctgctgg ataaatacgc cgatgcgggc
     2401 gtacaggaga tcgaaagtat tcaggtgctg aaacttaaac cattcgacag catgggcact
     2461 ttaccggaga ttattaaaac cggatttggc gaccgtaacg ggtataatca ggcgctcagc
     2521 gagctggaaa acgaaatcta ccaattaccg ccccgctctg cttaacaact ctcttttgtg
     2581 ccttctgcaa ccaacatgtt gcagaaggcc ttgtttttca tggaatacaa catgtctatc
     2641 agttcagtaa tcaaatcatt acaagatatt atgcgcaaag atgccggtgt ggatggcgat
     2701 gcgcagcgtc tcggtcagct ctcctggctg ctgtttttga aaatcttcga tgcccaggaa
     2761 gaggtgctgg aactggagca ggataactat caatatccga tcccacagcg ttatttatgg
     2821 cgcagttggg ccgcaaacgc tcagggcatt accggtgatt ccctgctgga attcgttaat
     2881 gatgatctgt tcccggcgtt gaaaaacctc actgcgccta tcgataaaaa cccacgcggc
     2941 tacgtggtga agcaggcgtt cagcgatgcc tataactata tgaaaaacgg tacgctactg
     3001 cgccaggtga tcaacaagct gaacgaaatt gactttacca gcgccagcga acgccatctg
     3061 tttggtgata tttacgaaca gatccttaaa gatctgcaat ctgcgggcaa tgctggcgaa
     3121 ttctatacgc cacgcgccgt cactcgcttt atggtggatc gcgttgatcc gaaactcggc
     3181 gaatccatta tggacccggc ctgcggtacg ggcggttttc ttgcctgcgc atttgatcat
     3241 gtaaagaaca aatacgtgaa gagcgtcgcc gatcatcaga cgctgcaaca acagatccac
     3301 ggtgttgaga aaaaacagct tccgcacctg ctggcgacca ccaatatgct gctgcacggc
     3361 attgaagtac cggtacaaat tcgtcacgac aacaccctga acaaaccgct ttcctcctgg
     3421 gatgaacaac tggatgtcat tgttaccaac ccgccgtttg gtggcacgga agaagacggt
     3481 attgagaaga actttccggc agagatgaaa acccgcgaaa cggcggatct gttcctgcaa
     3541 ctgattgtgg aagtactggc gaaaaacggt cgcgcggcgg tggtattgcc ggatggcaca
     3601 ctatttggcg aaggcgttaa aaccaaaatc aaaaaactgc ttaccgaaga gtgcaacctg
     3661 cataccatcg tgcgtttacc gaatggggtg tttaacccct ataccggcat taaaaccaac
     3721 ctgttgttct ttaccaaagg tcagccaacc aaagagattt ggttctatga gcatccgtat
     3781 ccggcgggcg taaaaaacta cagcaaaacc aagccgatga agtttgaaga gtttcaggcg
     3841 gagatcgact ggtggggtaa cgaggccgat ggttttgcca gccgcatcga gaatgagcaa
     3901 gcgtggaaag tcagcattga tgacgtgatt gcgcgtaact tcaatctgga tattaaaaac
     3961 ccacatcagg cggaaaccat tagccatgat ccggacgaac tgttagcgca gtatgcaaaa
     4021 cagcaggcgg agatccagac gctgcgtaat caactgcgcg atattcttgg cgctgcgctg
     4081 tctggcaagg aggttaacta atgagtgtgg aaaagctgat cgttgatcat atggaaacct
     4141 ggacctcggc gttgcaaacc cgttccaccg ccgggcgcgg cagctccggt aagattgatt
     4201 tgtatggtat taagaaattg cgtgagctga ttctggaact ggcggtgcga ggtaaactgg
     4261 tgccgcagga tccgaacgat gaaccggcgt cggagctgct gaagcgtatt gcggcggaaa
     4321 aagcagagct ggtgaagcaa ggtaaaatta aaaagcaaaa accgctgccg gaaattagcg
     4381 aggaagagaa gccgtttgaa ttgccggagg ggtgggaatg gactcgatta attaatcttg
     4441 gtatatgggc tttagggtct ggttttccaa atgtggtaca gggaagcact gataaagaaa
     4501 ttttgatgtg caaagttagc gacatgaatc ttgaaggtaa cgagaaattc attttttcga
     4561 caaaaaatac aatttcaaaa gacctagctg atg
//
LOCUS       DAZFRJ010000267         4516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000267 DAZFRJ010000000
VERSION     DAZFRJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.269"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(89..286)
                     /locus_tag="ACHKUN_004468"
     CDS             complement(89..286)
                     /locus_tag="ACHKUN_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6662585.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(528..1058)
                     /locus_tag="ACHKUN_004469"
     CDS             complement(528..1058)
                     /locus_tag="ACHKUN_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO6662586.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(1067..1426)
                     /locus_tag="ACHKUN_004470"
     CDS             complement(1067..1426)
                     /locus_tag="ACHKUN_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6662587.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(1439..2485)
                     /locus_tag="ACHKUN_004471"
     CDS             complement(1439..2485)
                     /locus_tag="ACHKUN_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6662588.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(2487..2759)
                     /locus_tag="ACHKUN_004472"
     CDS             complement(2487..2759)
                     /locus_tag="ACHKUN_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662589.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            2895..3152
                     /locus_tag="ACHKUN_004473"
     CDS             2895..3152
                     /locus_tag="ACHKUN_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6662590.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            3158..3457
                     /locus_tag="ACHKUN_004474"
     CDS             3158..3457
                     /locus_tag="ACHKUN_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6662591.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(3662..4006)
                     /locus_tag="ACHKUN_004475"
     CDS             complement(3662..4006)
                     /locus_tag="ACHKUN_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662592.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(4003..4368)
                     /locus_tag="ACHKUN_004476"
     CDS             complement(4003..4368)
                     /locus_tag="ACHKUN_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO6662593.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(4370..>4516)
                     /locus_tag="ACHKUN_004477"
     CDS             complement(4370..>4516)
                     /locus_tag="ACHKUN_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO6662594.1"
                     /translation="IVLMIPIPPLLLVWFIVKIIEPVIELYNDVVWASFSTLHNKINP
                     YKES"
BASE COUNT         1095 a         1228 c         1007 g         1186 t
ORIGIN      
        1 aaaaggtaaa ttatcatcaa cacataagca aaggactgac aggtgtcgcc cccccaccag
       61 ccgcccattc accacaaata aaaagccttc aggactgaag gcgtctgtaa caaccgcact
      121 gatagtctgc cagacccgcc ataacaagct gggtcagtat taactggcag cgttcgcgtg
      181 aaaggtaagt attctgcgca acctccccga ctgtcgccgg ttcggtaacg cttaattcat
      241 taaacaccac tctggcggtt tcggtcatat cctgctgttt cagcatgtct ttttcccttt
      301 ttcggttaac gtgacacacc aataactctt gtcgaaaaag ccagcaagct gaaagaacgg
      361 tattaataac caccagcgaa tttattgcgc tgctgtatat tacggacaca aaaaaaccac
      421 cttccggtgg cttccttgtg cgaaaaaact tgcatttcgc ctcgcgatac agctttgcga
      481 agcttacagg aattcaagct gtttctgcgt aaaaaagcaa gcttttttta tcgaaatgaa
      541 tcgtgcatag gtacataaag catgtgttca gccacggcta accaacctgc aatacgtttc
      601 tcacatgtgc tgaaacacca ttccgggtga gtacgattta aacattctgc catttttctc
      661 ttactcattc cccgtccttc gtacctttgc cggagaatat tgattagccc gggatattcc
      721 ccaagcacct cactgataac gcgatcaata atcaacgcct ctgtgtctgt acaatgtgac
      781 aaccagctct tctgcttccc tctggtcata tcccgaaaaa atgcctcaag ttccggtttt
      841 tccagcccgg atttcttcat gctgcgtaaa acctcattaa ctgctgtttt cgtcagcttt
      901 ttcgaaacca gtaaccggtt aaacatattt ccggatttac ccccaccgat atacgaccac
      961 cgcccccaca tccgtaattt cccctggatc cagactgctt ccagcgtgtt caggcgtaaa
     1021 tgttcgccgc ttttgcctgt aatttccgga tatatcatat ttacgctcac tcactctcaa
     1081 ttttgtaaat cttcacaccc agccgtccac cagatactgg ctgaccgcgc acaatattga
     1141 tttcatcaaa ctgctcgtca tcaatgagca cttccgcatg cgtcagcgca tccagcggtg
     1201 ctttcagaat gttgtccagg tcacggcggc gcttatccgg tggctctgca atcaccttta
     1261 tcgccagcct tccggacagg cttaatttca gccgctgctg gcgaacaata agcgccacag
     1321 cccggcgata acgctttccc tcctccgaga taaaatatgt gctgccacgg cgtcgccagt
     1381 aagtgttcac cgtcggcggg taaggtaaaa ccaaatctat gagcatcagt cacctctttt
     1441 acccaagcac gccagttgca aaggcgtgat caagaaaacg aaaaattaaa tcaacctgag
     1501 aaccatgctt ttcttcgaac gccagcggat ccgcatgaag ctcgttgtga tgctcccgac
     1561 acagcggtag cgtgaaaata tcgtgagatt ttgtccccat tcccccctga ccatgaccaa
     1621 tcaggtgatg cggatcgtca gcaggcttac cacaacacgc acacggctgt gtcttcaccc
     1681 agtgcgtgta tttctcgtta acccagcggc gacgtttagg tcgtttcatg aaagattccg
     1741 gagactcagg atcaacggca atgctgacca ccgtcttttc ctgtggcggt ttttgctggt
     1801 gagcgtgagg cagcggcgca agattttttg tgcgctgctt cagtatgctg gtggcggtct
     1861 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     1921 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     1981 accaggataa ttcagccaga gataattccc gttcctgtgc accacttatt gcgtgacgta
     2041 tgacgtcaat catccaggct gtcagatttt gttgagcaag ctgcccgagt gattcggatg
     2101 tctggtcgcg cagctggttg tcgcagtgcc agcacaacac cattgcgccg gtaccataac
     2161 ggtgaatgac ggtttcgctg tgatgataat cgccgtgtgg ccactggcag gatttaatat
     2221 ggcgcaacag ccagtcagac aatgcaccag caccaccagc agcacgaatc acccgtgcgt
     2281 tactgaaaaa cggcagcaat gttttgtctt ccactagcgg ctggcgaacg gcaggaacaa
     2341 ccccggacgg cagattacgc atgcttttcg gttccggctc caccagtacc cgggtattgt
     2401 ggaataccgg catggattca cgacccggct taacgatcac cagcccgagt tccggtacca
     2461 gaacaggtcg aagtaatacc cgcacgttac ctccagatgc gttgctggaa tgtgcgggac
     2521 ggacgcggcg ggcgttcgga ataaaggagc ctgacgtaga ttatccagtg acggtagtcg
     2581 aggctaaggg ctttcttaaa ctcataccca cgtctgcggt agttatgaat cagccattcg
     2641 gcctgttctt cagtgcaggg atcgtgctga taccagtcat atttgaatgt gtgagaacac
     2701 cgcccttgcc tgctggcagg ggcgatatca gaattgtgat gtttggtatt gtgcgccatc
     2761 ggttttctct gctggcgcag caggtgccag ttgttcaagc tggcgtgcgg caatattgtc
     2821 tctgatttct gttgtcgtca acaggcaacg tgctatcatc gaatggtgtt ctatcctact
     2881 ccgtgaggtt taccatgcgt acaacccaac aattcagcat tacattaact aacgaaatgg
     2941 ctgacatggt gcgcgcccgt gtggcttccg gtgcctatgc ttcagaaagc gaggtcattc
     3001 gtgaagggct tcgcgcactg aatgagcgcg ataaagcaat cgaagcgtgg ttaacgcatt
     3061 cagccgcccc ctctcttgat tctatccgcg aaaatccaaa caacggacgc tccatttcac
     3121 aggttcgcgc cgcgattcga tccgggaagt aatctgcatg acatatgaag tcatcattac
     3181 tcctgaggcc gaacaacaaa taatcaacct gcacaggtat ataacggaga aagcagggaa
     3241 cgtcattgct gacaattatg ccaatgcgct tcttgattat cttgatgggt tttctacatt
     3301 cccgcatcgg ggcaataaac gcgatgatat tcgccagggg atgcgggtaa ctcatttccg
     3361 ccacagaacg attattgctt ttgccgttga tagcagaaaa gtctttattg tcggtatcta
     3421 tcatggtggg caaagttatg aaaccgattt cttataaact tttacccaca tcattccggt
     3481 gttagaatta accgtccgcc ccctctctta ctggcggatt cgtaggctat ataaatcaaa
     3541 gatcccggct catgtttatg tcgggatctt ttttcggcga tttatcccca gcggcaaatc
     3601 gaatacacca ccagcgccac cgccatcgca attcctaccg ttgtgaatgc ttcaggccag
     3661 gtcatcgtaa aatatcctcc acgcttatca gtccgttccg ctccagataa ctcatcgcct
     3721 tatccggtaa tttgcagtct ggcttcgctt tcctcagttg ccaggttaac tgctttacca
     3781 gcatggttaa ctcatcgacc agacgctgat atcccactgg tttgtattca tgcaatttac
     3841 cggctggctc tgctgccagc gataccagtg cgatttccag aacagcaata tccatcttat
     3901 atgtgcggat gatgtcatgg tcgattgtgc ccggtatgca cagtctctgt gtttcaatag
     3961 tctcctctgc gtgagctatt aactgctctc tggtaaaagt cgtcatgccg tagccccttc
     4021 ttgatatttt tcaaaccaga acacaactgg gtcagatttc atttcaacca atcccatacg
     4081 aaccagcgct ttgcctttcc cggacgcaag gaattcacga cgaccatcac tgataattcg
     4141 ccgataatct tccagactac tgcaatgctt gtgcagattg catgggtggc atgccggaac
     4201 catgttggat atatcgtcac gttcctggtg aagcatattt ccatcaaaac gaatgaccgg
     4261 ttttacatgg tctgcatgcc acttttcgcc aagttcgcag ccgcaataag cacagcgacc
     4321 gccgaacttc atgcgcagct ctgcacgttg ttttttcgtc agtgccatat cagctttcct
     4381 tatacggatt aattttattg tgcagtgtgc tgaacgacgc ccacaccacg tcgttataca
     4441 attcaataac tggctcaatt attttcacga taaaccagac cagtaatagc gggggtatcg
     4501 gtatcatcaa cacgat
//
LOCUS       DAZFRJ010000268         4495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000268 DAZFRJ010000000
VERSION     DAZFRJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.270"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..119)
                     /locus_tag="ACHKUN_004478"
     CDS             complement(<1..119)
                     /locus_tag="ACHKUN_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6662595.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFP"
     gene            complement(234..545)
                     /locus_tag="ACHKUN_004479"
     CDS             complement(234..545)
                     /locus_tag="ACHKUN_004479"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6662596.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            744..1442
                     /locus_tag="ACHKUN_004480"
     CDS             744..1442
                     /locus_tag="ACHKUN_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6662597.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1487..2386)
                     /gene="eamA"
                     /locus_tag="ACHKUN_004481"
     CDS             complement(1487..2386)
                     /gene="eamA"
                     /locus_tag="ACHKUN_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO6662598.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2581..3768
                     /gene="ydeE"
                     /locus_tag="ACHKUN_004482"
     CDS             2581..3768
                     /gene="ydeE"
                     /locus_tag="ACHKUN_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO6662599.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3895..3990
                     /gene="mgtS"
                     /locus_tag="ACHKUN_004483"
     CDS             3895..3990
                     /gene="mgtS"
                     /locus_tag="ACHKUN_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO6662600.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3990..4094
                     /gene="mgtT"
                     /locus_tag="ACHKUN_004484"
     CDS             3990..4094
                     /gene="mgtT"
                     /locus_tag="ACHKUN_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO6662601.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(4209..>4495)
                     /gene="dgcZ"
                     /locus_tag="ACHKUN_004485"
     CDS             complement(4209..>4495)
                     /gene="dgcZ"
                     /locus_tag="ACHKUN_004485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO6662602.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         1219 a         1062 c          946 g         1268 t
ORIGIN      
        1 ggaaacgtaa aaacgaatcc atctctcacc gctgcaacat gtttttgtga gtttaacttg
       61 atagcaactg gaacaagaaa acgttccatt ccacgttcga atgaggccat taatcccatt
      121 tttttatacc tgttgttttg gatatttata catcccaata aagcatgtat gccatatcca
      181 ttttatgatg tgtgaaaagt tagttcaggt aatagtttaa taatattaga ttcttactct
      241 tgcagtagtt tcatcgcaaa ttcaagaact gcctgtccgt tcatggtgcc gtagtctttc
      301 atctcgataa cggctacagg cattttcccc tgtgcaacgg cttcaatctc tggtttctga
      361 aagcgaactt gcgggccaag taaaacaacg tcaacttcgt tatttttaat tttcccttta
      421 gcctctgcaa tcgccaatgc ggaaatatta acttcaagtg aaatagcgtt agcatgatca
      481 atcatacgtt taaccagcat actggttgac atgcccgcag cgcaaactaa caatattttt
      541 ttcatatttt accttactgg tatataacag attacattgt ttaaatatca tatcccgcag
      601 tagctatacg tcctggtaca ttgatcacag taatacagtg ataaagttgt tcaacatcag
      661 ggtgattatt aaacgactcg gccatacgat aaaccaatca ggactcaact ccttgttttc
      721 aatggctctt aaaaggtgca aaagtgattt ttcaaaagat tgcccgctta cttaaatccg
      781 aaatcaatgg caattcatgg catgttggtg atttgttgcc gtcagaagcg gaactcgctg
      841 ttcgttataa tgtttcgcgt aataccctgc gtaaggcatt atccttgctg gaaggcgaag
      901 gaattattca cagaaagcat ggttcaggaa gatacattca aaaaaagaat tttgttgcac
      961 acattgatca catgaacagt ttcagtgaaa ttgcgcataa aagcggcaaa gaggcaggga
     1021 gtcagattat gaaatttgaa gtgcaggatg cttcacccac tattgcgact gagctgaatt
     1081 tagtgactgg tgagcaggtt tattacataa agagactgcg attcattgag gataatgcag
     1141 cacaattaga agaaacgtgg atgtcagtag cacgttttcc tgatttaacc gtgtcacata
     1201 tgcaaaaatc caaattttca tatattgaga atgaatgcgg gatcaaaatc attggcacct
     1261 ttgaaacttt ctcccctact tttcctaccc cagaaatcgc cagtatttta cggatcagcc
     1321 cacgggatcc catacttaaa attcagaccc aggctgtgga cagtaactct attccgctgg
     1381 attattcgtt actttacagc aatattttcg agttccaggt aaagtacttt ttcccgcgat
     1441 aaaagcagac gcacagagtg ctgacatcac attctgtgcg tatggtttag cctctaacct
     1501 ttaccgcttt acgccaccgc aagccaaata cattgatata cagcccggtc ataatgagca
     1561 ccgcacctaa aaattgcaga cccgttaagc gttcatccaa caatagtgcc gcacttgcca
     1621 gtccgactac gggcaccagt aacgataacg gtgcaacccg ccaggtttca tagcgtccca
     1681 gtaacgtccc ccagatccca taaccaacaa ttgtcgccac aaacgccaga tacatcagcg
     1741 acaagatggt ggtcatatca atagtaacca gactgtgaat catggatgcg gaaccatcga
     1801 gaatcagcga ggcaacaaag aagggaatga ttgggattaa agcgctccag attaccagcg
     1861 acatcaccgc cggacgcgtt gagtgcgaca tgatcttttt attgaagatg ttgccacacg
     1921 cccaactaaa tgctgcagcc agggtcaaca taaagccgag catcgccaca tgctgaccgt
     1981 tcagactatc ttcgattaac accagtacgc caaaaatcgc taaggcgatc cccgccaatt
     2041 gtttgccatg cagtcgctcc ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct
     2101 gtgcctgtaa caccagcgaa gccagtccag caggcatacc aaagttaatg gcgcagaaca
     2161 gaaaggcaaa ctgcgcaaaa ctgatggtta atccataccc caagagcaaa ttcagtggta
     2221 ctttcggtcg tgcaacaaaa aagatggccg gaaaagcgac cagcataaag cgcaaaccgg
     2281 ccagcatcag cggtggcatg ttatgaagcc ccactttgat gaccacaaaa tttagccccc
     2341 atacgaccac taccagtagc gccaacaccc catcttttcg cgacattcta ccgcctctga
     2401 atttcatctt ttgtaagcaa tcaacttagc tgaatttact tttctttaac agttgattcg
     2461 ttagtcgccg gttacgacgg cattaatgcg caaataagtc gctatacttc ggatttttgc
     2521 catgctattt ctttacatct ctaaaacaaa acataacgaa acgcactgcc ggacagacaa
     2581 atgaacttat ccctacgacg ctctaccagc gcccttcttg cctcgtcgtt gttattaacc
     2641 atcggacgcg gcgctacgct gccatttatg accatttact tgagtcgcca gtacagcctg
     2701 agtgtcgatc taatcggtta tgcgatgaca attgcgctca ctattggcgt cgtttttagc
     2761 ctcggttttg gtatcctggc ggataagttc gacaagaaac gctatatgtt actggcaatt
     2821 accgccttcg ccagcggttt tattgccatt cctttagtga ataacgtgac gctggtggtg
     2881 ctcttttttg cccttattaa ctgcgcctat tccgtttttg ctaccgtgtt gaaagcctgg
     2941 tttgccgaca atctttcgtc caccagcaaa acgaaaatct tctcaatcaa ctacaccatg
     3001 ctaaacattg gctggaccat cggtccgccg ctcggcacgc tgttggtgat gcagagcatc
     3061 aatctgccct tttggctggc agctatctgt tccgcgtttc ccatgctttt cattcaaatt
     3121 tgggtaaagc gcagcgagaa aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa
     3181 gttttattac aagataaagc actgttgtgg tttacctgct ctggttttct ggcttccttt
     3241 gttagcggcg catttgcttc atgcatttca caatatgtga tggtgattgc tgatggagat
     3301 tttgccgaaa aggtggtcgc ggttgttctt ccggttaatg ctgccatggt agttacgttg
     3361 caatattccg tgggccgccg ccttaacccg gttaacatcc gcgcgctgat gacagcaggc
     3421 accctctgtt tcgtcatcgg tctggttggt tttatttttt ccggcaacag cctgctattg
     3481 tggggtatgt cagctgcggt atttactgtc ggtgaaatca tttatgcgcc gggcgagtat
     3541 atgttgattg accatattgc gccgccagga atgaaagcca gctatttttc tgcccagtct
     3601 ttaggctggc ttggtgctgc aattaaccca ttagtgagtg gcgtagtgct aaccagcctg
     3661 ccgccttcct cgctgtttat catcttagcg ttggtgatca ttgctgcgtg ggtgctgatg
     3721 ttaaaaggga ttcgtgcaaa accgtggggg cagcccgcgc tttgttgatt taagtcgaac
     3781 acaataaaaa tttaattcag ccttcgttta ggttacctct gctaatatct ttctcattga
     3841 gatgaaaatt aaggtaagcg aggaaacaca ccacaccata aacggaggca aataatgctg
     3901 ggtaatatga atgtttttat ggccgtactg ggaataattt tattttctgg ttttctggcc
     3961 gcgtatttca gccacaaatg ggatgactaa tgaacggaga taatccctca cctaaccggc
     4021 cccttgttac agttgtgtac aaggggcctg atttttatga cggcgaaaaa aaaccgccag
     4081 taaaccggcg gtgaatgctt gcatggatag atttgtgttt tgcttttacg ctaacaggca
     4141 ttttcctgca ctgataacgg atcgttgaca cagtagcatc agttttctca atgaatgtta
     4201 aacggagctt aaactcggtt aatcacattt tgttcgtcaa taaacatgca gcgatttctt
     4261 ccggtttgct taccctcata cattgcccgg tctgctcttc caatgaccac atccagaggc
     4321 tcttcaggaa atgcgcgact cacacctgct gtcacggtaa tgttgatatg cccttcagaa
     4381 tgtgtgatgg catggttatc gactaactgg caaattctga cacctgcacg acatgcttct
     4441 tcatcattag ccgctttgac aataatgata aattcttcgc ccccgtagcg ataaa
//
LOCUS       DAZFRJ010000269         4489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000269 DAZFRJ010000000
VERSION     DAZFRJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.271"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..293)
                     /gene="chbB"
                     /locus_tag="ACHKUN_004486"
     CDS             complement(<1..293)
                     /gene="chbB"
                     /locus_tag="ACHKUN_004486"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO6662603.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVA"
     gene            complement(592..930)
                     /gene="osmE"
                     /locus_tag="ACHKUN_004487"
     CDS             complement(592..930)
                     /gene="osmE"
                     /locus_tag="ACHKUN_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO6662604.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1132..1959
                     /gene="nadE"
                     /locus_tag="ACHKUN_004488"
     CDS             1132..1959
                     /gene="nadE"
                     /locus_tag="ACHKUN_004488"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO6662605.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2189..3076
                     /gene="cho"
                     /locus_tag="ACHKUN_004489"
     CDS             2189..3076
                     /gene="cho"
                     /locus_tag="ACHKUN_004489"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO6662606.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3036..3611)
                     /gene="ves"
                     /locus_tag="ACHKUN_004490"
     CDS             complement(3036..3611)
                     /gene="ves"
                     /locus_tag="ACHKUN_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO6662607.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3814..4299)
                     /gene="spy"
                     /locus_tag="ACHKUN_004491"
     CDS             complement(3814..4299)
                     /gene="spy"
                     /locus_tag="ACHKUN_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO6662608.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1120 a         1089 c         1087 g         1193 t
ORIGIN      
        1 gcaaccgcag ccttaagcac gcctaaacca tcgactttgc cataaagcag cgagtcaatt
       61 acttcaaccg gtttgttggg taacaaacgc tggatttcgg gcaacatata agcaatctgc
      121 ggccctaata acacgacatc ggcattctga cctttttcac cagccagtgt ttccggaaat
      181 gcttcaataa tgaccggaac ttcatatttt tctgcctgtg cgcgcatttt tgataccagt
      241 aaagaggtag acatgcccgc agaacaaaac agataaatgt gtttcttttc cataaaactg
      301 ccctcatcga cgattatctg tcagccagac actccgcaag ccttaacctg cttccatgct
      361 ctgggtaact tgcgaaacca acatgacttt ttttgtggct gaaaggagta tacggtatag
      421 acctgagcaa tagtatttct ttgaccttat aaattgtttc tccttgacct gtggttatga
      481 ccctcttcac atttcgggca aataattcgc gaagataatt aagaactcag gccaaaaaaa
      541 accggcgcaa tggccggttt ccgttgttac tcaagctttc agacgaattg attacttcgc
      601 agcctgtgga tcagtgtcgt attcagcaca ggtctgataa ccggagttga tgacatgtcc
      661 ggtatcatct aacgcgacaa agtaggtttc tgctttacca tcacgttgac ccaggatgta
      721 ggtctggcaa gtaccgcgag catggatcat gctcacttca gacgaaggtt tacccgcaat
      781 ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct ttcaccacag gctgtacaaa
      841 ctggtctttg gtacgatcat aagccgtaca acccgccagc atggttaata ccgccgctgc
      901 actcagaatt cctgccatat tcttgttcat attccgtcct cttgtttatc agcgtgttag
      961 ataagcctgg aatacattgg gcgctttttc aagcccgtga acgaaacggc tccgctttca
     1021 gaggattcct gtatgacgtt ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta
     1081 gcaacgggtt agctttaagg aagttttgtc ttttctgtct ggaggggttc aatgacattg
     1141 caacaacaaa taataaaggc gctgggcgca aaaccgcaga ttaatgctga agaggaaatt
     1201 cgtcgtagtg tcgattttct gaaaagctac ctgcaaactt atccgttcat taaatcactg
     1261 gtgctcggga tcagcggcgg tcaggactcc acgcttgccg gaaagctgtg ccagatggcg
     1321 attaatgagc tgcgccagga aactggcaac gaatcactgc aatttattgc cgtacgcctg
     1381 ccctatggtg ttcaggctga cgaacaagat tgccaggatg ccattgcctt tattcaaccg
     1441 gatcgcgtat taaccgttaa tatcaagggc gcggtattgg ctagcgagca ggcattgcgg
     1501 gaagcaggca ttgaactgag cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg
     1561 aaagcacaat atagcattgc gggtatgacc agcggtgtcg tggtgggcac cgatcatgca
     1621 gcagaagcca ttaccggatt cttcactaaa tatggtgacg gcggtacgga tattaatccg
     1681 ctgtatcgtc tcaacaaacg ccagggtaaa cagttgctga cggcattagg ttgcccggaa
     1741 cacctttata agaaagcccc aacggccgat ctggaagatg atcgcccttc tctgccagat
     1801 gaagtggcac tcggcgtgac ctatgacaat atcgacgact atctggaagg gaaaaacgta
     1861 cctgaacagg tcgccagaac aatagagaac tggtatctga aaaccgaaca taaacgccgt
     1921 ccgccaatta ccgttttcga tgatttctgg aaaaagtaat aatttgcaca taatggtttg
     1981 tgcgaaaaag agtaatttgt tcacgccgga tgcggcgtga acgccttatt cgacctacaa
     2041 aactatgcaa attcaatata ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag
     2101 acggtgtaac ctttgtcatc gacccgcctc ttttttaatc gcttcccgcc tgttacactg
     2161 gatagataac cagcattcgg agtcaacagt ggtacggcgt ttaacttctc cgcggctcga
     2221 atttgaagct gcggcaattt atgaatatcc cgaacattta cgttcattcc ttaatgactt
     2281 acccacccga ccaggggtgt atctgtttca tggcgaaagt gacaccatgc cgctctatat
     2341 cggcaaaagc gttaatatcc gcagtcgggt gctttctcat ttacgcaccc cggatgaagc
     2401 cgccatgcta cggcaatccc ggcgaattag ctggatctgc accgcaggcg aaatcggcgc
     2461 tctgctcctt gaagcgcgat taatcaaaga acaacagccg ctgtttaata aacggttgcg
     2521 ccgcaatcgc cagctctgtg ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc
     2581 caaagaggtg gatttttcac gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc
     2641 agctttgcaa gcattgcaga gcatcgctga tgaacaaaaa ctttgttatg gcctgctggg
     2701 actggaaccg ttaagtcgcg gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg
     2761 agcatgctgc ggtaaagaga gccatgagga acatgcgcta cgcttgcgcc agtctctgga
     2821 gcgtttgcgg gtggtgtgtt ggccttggca aggggcggtg gcgctgaaag aacagcaccc
     2881 ggaaatgact caatatcata ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct
     2941 ggaagacgcg acaacgttaa ttcggacacc cgccgggttt gatcacgacg gttataaaat
     3001 tctttgtaag ccgctgcttt ccggtaacta tgaaattact gaacttgatc cggcgaatga
     3061 ccagcgagcc agttgatttc actgaacaac agtttacctt gcggttgtaa aagccgcagg
     3121 gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag tgagcagttt gtcgcccagt
     3181 tgccacgccc cgttaatcac aaacactacc ccgccgcgtg agccaaaggt agtaaacgta
     3241 cgttcggcaa ttctgacttt cgccttacag acatccaggc gcgtcataat attgaaatcc
     3301 atcgacatct gccctgccgt cagtttcgct tttaccacct ggtccgccgc aaaggcaaaa
     3361 ggctgtaacg gttttaaggt atggttaaag tggtctgcgc tttcaaggaa catctcgccg
     3421 ccttccagca acgtcactat cctttccata ccgggaaaca aagaaaactc cccattcgcc
     3481 gcgatggatg caatgctggc acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa
     3541 atttcgcgcg tttcaccggc agcatttcgc cacagattca ccgacatttt acgcatatca
     3601 aagtattcca tactcgctcc cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg
     3661 atggttatcg gcaactcggg tgatttacgt tagtggtgat caggcttttt ttacaactgt
     3721 ttgagaagag aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca
     3781 agaccggcgg tcttaagttt tttggctgaa agattattca gcagttgcag gcattttacc
     3841 ttttgccgct ggacgttctg tcagacgctt ctcaaaatta gcattaaatt gctttttctg
     3901 ttccggcgtc aggatgttgt aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc
     3961 tttgcgctgt tcttccattt ttgcgatctg cgcttcagct tttgctttat cgaaggtatc
     4021 gctggcaacg atgtcatgca ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc
     4081 acgctggcct ttcatgattt cgcggatctg ctgtttctgc gcgtcggtca ggttcaggtc
     4141 tttgaacatc atgtcctgat gcggaccgaa cttgcctttg tggtgcataa tcggcttcgc
     4201 gtcagccggt gctgcggtag tggtgtctgc ggcatgggcc aggttagccg cgccaagagc
     4261 cagggtagag gcaacaaaca gtgcagttaa tttacgcata ttctatatcc ttcctttcag
     4321 ttatttatta cggctttctt aagtagcgtg ccgtgttgac gagattaact ttactgactt
     4381 tagcgtcaat taatcagagc aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc
     4441 aattatggag aaaaaaagaa aatttgatgg agagtgatga gagaatatt
//
LOCUS       DAZFRJ010000270         4481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000270 DAZFRJ010000000
VERSION     DAZFRJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.272"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            174..1331
                     /gene="acrE"
                     /locus_tag="ACHKUN_004492"
     CDS             174..1331
                     /gene="acrE"
                     /locus_tag="ACHKUN_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO6662609.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1343..4447
                     /gene="acrF"
                     /locus_tag="ACHKUN_004493"
     CDS             1343..4447
                     /gene="acrF"
                     /locus_tag="ACHKUN_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO6662610.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1079 a         1090 c         1189 g         1123 t
ORIGIN      
        1 tagaatatta atcttttttt gtgtttatgt gccttgagac gccagtattc ataactattc
       61 cttacatcga cgaatgataa tttgtaggat agcaaactgt atttttcttt ctgcgagtta
      121 acgcgttacc tttctgggta aataacgcgc ttttggtttt ttgaggaata gtaatgacga
      181 aacatgccag gtttttcctc ctgccctcct ttattctgat ctccgcggct ttaatcgccg
      241 gttgtaacga taagggagaa gagaaagctc acgtcggtga accgcaggtt accgttcata
      301 ttgtgaaaac ggccccgtta gaagttaaga ctgaattacc aggccgcacc aatgcttacc
      361 gtatagccga agttcgccca caggttagcg ggatcgtact gaatcgtaat ttcactgaag
      421 gcagcgatgt acaagcaggc cagtccctgt accagatcga tcccgcgacc tatcaggcaa
      481 attatgacag cgcgaaaggc gaactggcga aaagtgaagc cgccgccgcc atcgcgcatt
      541 tgacggtaaa acgttacgtt ccgctcgtgg gtacgaaata catcagccag caggagtacg
      601 accaggccat tgctgatgct cgccaggccg atgccgccgt gattgccgca aaagccacag
      661 tcgaaagcgc tcgcatcaat ctcgcttata ccaaagtgac tgcgccaatt agcggacgta
      721 tcggcaaatc gaccgtgacc gaaggcgctc ttgtcactaa tgggcaaacg actgaactgg
      781 cgactgtcca gcagctcgat cctatctacg ttgatgtgac ccaatccagc aacgatttta
      841 tgaggctgaa gcaatccgta gagcaaggaa atttgcataa ggaaaacgcc accagcaacg
      901 tagagttggt catggaaaac ggtcaaacct atcccctgaa aggtacgctg caattctctg
      961 atgtgaccgt tgatgaaagc accggctcca taaccctacg tgctgtcttc cctaacccgc
     1021 aacatacgct tttgccgggt atgtttgtgc gtgcacggat tgatgaaggc gtccaacctg
     1081 acgccattct tatcccgcaa caaggcgtta gccgcacacc gcgtggtgat gcaaccgtgc
     1141 tgattgttaa cgataaaagt caggtggaag cgcgccctgt cgttgccagt caggcgattg
     1201 gcgataaatg gttgattagc gaaggtctga aatctggcga tcaagtcatt gtcagcggcc
     1261 tgcaaaaagc gcgtccggga gagcaggtta aagccactac cgataccccc gcagatactg
     1321 catcgaagta aggtaatctg acatggcaaa cttttttatt cgacgaccga tatttgcatg
     1381 ggtgctggcc attattctga tgatggcggg cgcactggcg atcctacaat tgcccgtcgc
     1441 tcagtatcca accattgcac cgcctgcggt ttctgtttcg gcaaactatc caggtgctga
     1501 tgcgcagacc gtgcaggata cggtgacgca ggttatcgaa cagaatatga atggtatcga
     1561 taacctgatg tatatgtcct ccaccagcga ttccgccggt agcgtgacaa ttacccttac
     1621 cttccagtcc gggaccgatc ctgatatcgc gcaagtgcag gtgcagaaca aactccagct
     1681 cgccacgccg ttgctgccgc aggaggttca gcagcagggg atcagtgttg aaaagtccag
     1741 tagcagctat ttgatggtgg cgggctttgt ctctgataac ccagacacca cacaggacga
     1801 tatctcggac tatgtggcct ctaacgttaa agatacgctt agccgtctga atggcgtcgg
     1861 tgacgtacag cttttcggcg cacagtatgc gatgcgtatc tggctggacg ccgatctgct
     1921 aaacaaatat aaactgacac cggttgatgt gattaaccag ttgaaggtac agaacgatca
     1981 gatcgctgcc ggacagttgg gcggaacgcc agcgttacca gggcaacagt tgaatgcctc
     2041 gattattgct caaacgcggt taaaaaatcc ggaagaattc ggcaaagtga ccctgcgcgt
     2101 aaacagtgac ggctcggtgg tacgcctgaa agatgtcgca cgggttgaac ttggcggtga
     2161 aaactataac gttatcgctc gtatcaacgg aaaaccggcg gcgggcctgg ggattaagct
     2221 ggcaaccggc gcgaatgctc tcgataccgc gaaagccatt aaggcaaaac tggcggaatt
     2281 acagccattc ttcccgcagg gaatgaaggt tctctaccct tatgacacca cgccattcgt
     2341 ccagctttct attcacgaag tggtaaaaac gctgttcgaa gccattatgc tggtgttcct
     2401 ggtgatgtat ctgttcttgc agaatatgcg agcaacgctg atccccacca ttgcggtacc
     2461 cgtggtgttg ttagggacgt ttgccatcct cgccgctttt ggttactcca tcaacacact
     2521 aacgatgttc gggatggtgc ttgccatcgg gctgctcgtc gatgatgcga tagtggtggt
     2581 ggagaacgtc gagcgcgtga tgatggagga taagctcccg ccaaaagaag cgacggaaaa
     2641 atcaatgtcg caaatccagg gcgcactggt gggtatcgcg atggtgctgt cagcggtatt
     2701 tattccgatg gcattcttcg gcggttctac tggggcaatt tatcgccagt tctctatcac
     2761 catcgtttcg gcaatggcgc tttctgttct ggtggcattg attcttaccc ctgcgttatg
     2821 tgcaacgctg cttaaacccg tctctgctga gcatcacgaa aataagggcg gtttcttcgg
     2881 ctggtttaat actaccttcg atcatagcgt taaccactac accaacagcg tcggcaaaat
     2941 cctcggttcc acaggacgat atttactgat ctatgcgctg attgttgcag gaatggtggt
     3001 gttgttttta cgtcttccgt cttccttctt acctgaagag gatcagggtg tctttctgac
     3061 catgattcag ttacccgctg gcgcgacgca agagcggacg caaaaagtgt tggatcaagt
     3121 gacggattac tatctgaaga acgagaaagc gaacgttgaa agtgtcttta cggttaacgg
     3181 ctttagcttc agcggccagg cacaaaacgc cggtatggcc ttcgtcagtc tgaaaccgtg
     3241 ggaagagcgt aatggtgatg aaaacagtgc ggaagcggta atccatcgtg ccaaaatgga
     3301 atttggcaag atccgcgacg gttttgtcat tccattcaat atgccagcca ttgttgaact
     3361 gggcacggca acgggtttcg actttgagtt aattgatcag gctgggctgg gtcacgatgc
     3421 cctaacccag gcccgtaacc agttgcttgg tatggcggcg caacatcctg ccagcttagt
     3481 cagcgtgcgt cctaatggcc tggaagacac cgcgcagttt aaactggaag ttgaccagga
     3541 aaaggcgcag gcattaggtg tttcactttc tgacatcaat cagaccattt caacggcgtt
     3601 gggtgggact tacgttaacg acttcatcga ccgtggtcgc gtgaaaaagg tgtatgttca
     3661 ggcggatgcc aaattccgta tgctgccaga agatgtcgat aaactttatg tccgcagcgc
     3721 caacggcgaa atggtgccat tctcggcctt taccacttca cattgggtgt atggctctcc
     3781 gcgactggaa cgctacaacg gtctgccgtc aatggagatt cagggggaag ccgcgccagg
     3841 aaccagttcc ggcgatgcca tggcgttgat ggaaaacctt gcgtcaaaat tacctgctgg
     3901 cattggttat gactggacgg gtatgtcgta tcaggaacgc ttatcgggaa accaggctcc
     3961 cgctctggta gcaatttcct ttgtggttgt tttcctgtgc cttgctgcac tctatgaaag
     4021 ctggtcaatt cctgtctcgg ttatgttggt cgtgccgtta gggattgtcg gcgtgctgct
     4081 ggcggcgaca ctctttaatc aaaaaaatga cgtctacttt atggtgggct tgctaacgac
     4141 aattggcttg tcggccaaaa acgctatttt gattgttgag ttcgctaaag atctcatgga
     4201 gaaagagggt aaaggtgttg ttgaagcgac actaatggca gtacgtatgc gtctgcgtcc
     4261 tatcctgatg acctctctcg cctttattct cggcgtatta ccgctagcta tcagtaacgg
     4321 tgccggcagt ggcgcgcaga acgcagtggg tatcggggta atgggcggaa tggtctctgc
     4381 aacgttgctg gcaatcttct tcgtaccggt gttctttgtg gtgatccgcc gttgctttaa
     4441 aggataaatc agaaacataa aggcactttc gggtgccttt a
//
LOCUS       DAZFRJ010000271         4474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000271 DAZFRJ010000000
VERSION     DAZFRJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.273"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..462
                     /locus_tag="ACHKUN_004494"
     CDS             <1..462
                     /locus_tag="ACHKUN_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6662611.1"
                     /translation="AINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEI
                     AKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEA
                     TDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            618..2153
                     /gene="gadC"
                     /locus_tag="ACHKUN_004495"
     CDS             618..2153
                     /gene="gadC"
                     /locus_tag="ACHKUN_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO6662612.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2284..3603
                     /locus_tag="ACHKUN_004496"
     CDS             2284..3603
                     /locus_tag="ACHKUN_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO6662613.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3980..>4474
                     /gene="dosC"
                     /locus_tag="ACHKUN_004497"
     CDS             3980..>4474
                     /gene="dosC"
                     /locus_tag="ACHKUN_004497"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO6662614.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKE"
BASE COUNT         1114 a         1093 c         1085 g         1182 t
ORIGIN      
        1 gccatcaact tctcccgccc ggcgggtcag gtaattgcac agtactatga attcctgcgc
       61 ctcggtcgtg aaggctatac caaagtacag aacgcctctt accaggttgc cgcttatctg
      121 gcggatgaaa tcgccaaact ggggccgtat gagttcatct gtacgggtcg cccggacgaa
      181 ggcatcccgg cggtttgctt caaactgaaa gatggtgaag atccgggata caccctgtac
      241 gacctctctg aacgtctgcg tctgcgcggc tggcaggttc cggccttcac tctcggcggt
      301 gaagccaccg acatcgtggt gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt
      361 gctgaactgt tgctggaaga ctacaaagcc tccctgaaat atctcagcga tcacccgaaa
      421 ctgcagggta ttgcccagca gaacagcttt aaacatacct gataacgttt aacggtaacg
      481 gtgtcccgaa acgaacccgt ttcgggacaa tttccaaagt ctgttcactg gcattagcaa
      541 cggaaaatat tgttctgaat acgcttcaga acaaaacagg tgcggttccg acaggaatac
      601 cgttttaggg ggataatatg gctacatcag tacagacagg taaagctaag cagctcacat
      661 tactcggatt ctttgccata acggcatcga tggtaatggc tgtttatgaa taccctacct
      721 tcgcaacatc gggcttttca ttagtcttct tcctgctatt aggcgggatt ttatggttta
      781 ttcccgtggg actttgtgct gcggaaatgg ccaccgtcga cggctgggaa gaaggtggtg
      841 tcttcgcctg ggtatcaaat actctggggc cgagatgggg atttgcagcg atctcatttg
      901 gctatctgca aatcgccatt ggttttattc cgatgctcta tttcgtgtta ggggcactct
      961 cctacatcct gaaatggcca gcgctaaatg aagaccccat taccaaaact attgcagcac
     1021 tcatcattct ttgggcgctg gcattaacgc agtttggtgg cacgaaatac acggcgcgaa
     1081 ttgctaaagt tggcttcttc gccggtatcc tgttacctgc atttattttg atcgcattag
     1141 cggctattta tctgcactcc ggtgcccccg ttgctatcga aatggattcg aagaccttct
     1201 tccctgactt ctctaaagtg ggcaccctgg ttgtatttgt tgccttcatt ttgagttata
     1261 tgggcgtaga agcttccgca acccacgtca atgaaatgag taacccaggg cgcgactatc
     1321 cactggctat gttactgctg atggtggcgg caatctgctt aagctctgtt ggtggtttgt
     1381 ctattgcgat ggtcattccg ggtaatgaaa tcaacctctc cgcaggggta atgcaaacct
     1441 ttaccgttct gatgtcccat gtggcaccgg aaattgagtg gacggttcgc gtgatctccg
     1501 cactgctgtt gctgggtgtt ctggcggaaa tcgcctcctg gattgttggt ccttctcgcg
     1561 ggatgtatgt cacagcgcag aaaaacctgc tgccagcggc attcgctaaa atgaacaaaa
     1621 atggcgtacc ggtaacgctg gtcatttcgc agctggtgat tacttctatc gcgttgatca
     1681 tcctcaccaa taccggtggc ggtaacaaca tgtccttcct gatcgctctg gcgctgacgg
     1741 tggtgattta tctgtgtgct tatttcatgc tgtttattgg ctacattgtg ttggttctta
     1801 aacatcctga cttaaaacgc acatttaata tccctggtgg taaaggggtg aaactggtcg
     1861 tggcaattgt cggtctgctg acttcaatta tggcgtttat tgtttccttc ctgccgccgg
     1921 ataacatcca gggtgattct accgatatgt atgttgaatt actggttgtt agtttcctgg
     1981 tggtacttgc cctgcccttt attctctatg ctgttcatga tcgtaaaggc aaagcgaata
     2041 ccggcgtcac tctggagcca atcaacagtc agaacgcacc aaaaggtcac ttcttcctgc
     2101 acccgcgtgc acgttcacca cactatattg tgatgaatga caagaaacac taaccatgtc
     2161 tgaatggtta taagacaagg gagcgataag tcatcgctcc ctttttcgtg cttgctgtcg
     2221 tattgactta accgggataa gtacgagaat gagcgcacat ctgtttaccg gaaaccagca
     2281 catatggata tctgctcccg aaacaagaaa ttaacgatta gaagaccagc gatactagtt
     2341 gcactggcac ttttactgtg tagttgtaaa agcacgcctc cagagtccat ggtgacacca
     2401 ccagcaggtt caaagccacc agccacgacg caacaatcgt cacaaccgat gcgtggcatc
     2461 tggctggcca cggtttctcg gctcgactgg ccaccggttt cctcggttaa cattagtaac
     2521 cccaccagcc gggcccgtgt acaacaacag gcgatgatcg acaaactgga tcatctgcaa
     2581 cgtctcggca taaacacggt ctttttccag gtcaagccgg acggtaccgc cctgtggcca
     2641 tcgaaaattt tgccgtggtc cgatcttatg accggtaaga ttggtgaaaa tccgggttac
     2701 gatccgctgc aatttatgct cgacgaagcc cacaagcgtg ggatgaaagt acacgcctgg
     2761 tttaacccct atcgcgtatc ggttaatacg aagcccggta ctatcaggga actgaatagc
     2821 actctgcctc aacaaccggc gagcgtctat gtgcaacacc gcgactggat cagaacgtct
     2881 ggcgatcgct ttgtcctcga cccgggcatc cctgaggttc aggactggat cacatcaata
     2941 gtcgcagaag tggtttcccg ctatcccgta gatggcgtgc agtttgacga ctatttctat
     3001 acggagtcac cgggttcacg gctaaatgat aacgaaacgt accgtaaata cggaggcgca
     3061 tttgcgtcaa aagcagactg gcggcgcaac aatactcagc agttaattgc aaaggtatcg
     3121 cacaccatta aaagcattaa gccgggagtc gaatttggtg ttagcccggc aggcgtgtgg
     3181 cgtaaccgat cacacgatcc gctcggttcc gatacccgag gcgcggcagc ctatgacgaa
     3241 tcctacgctg acacccgtcg atgggtggaa caaggattgc tggattacat tgctccccaa
     3301 atttactggc cgttctcacg gagtgccgcg cgttatgacg tgttggcaaa atggtgggcg
     3361 gatgtcgtta aaccgaccag gacccgcctg tatatcggta tcgccttcta taaagtgggt
     3421 gaaccttcaa agatagagcc agactggatg attaacggcg gcgtacagga actgaaaaag
     3481 cagctcgatc ttaacgatgc ggtaccagaa attagcggca ccatcttgtt ccgtgaggac
     3541 tatctgaata aaccacagac tcaacaagcg gtcagctatc tgcaaagtcg ttggggcagt
     3601 taaactccca ttcttacttt gtaacaagcc ggtgctttac ccaccggctt gttaccctct
     3661 tgaactatcc cactactaat catgcttact aaagtcaaat taaccacgca attaaccaca
     3721 cttagttaaa cagccataaa tcccctaata aaaatgtatg aatatgaaaa aacatacgat
     3781 attcgctgtc accgaaaaat aattacgatg ggctatactc ttctgaagaa cagtagcgta
     3841 gattaaaact gtcctaagtc gcttatttct tttcagtata tcttcatatt tcaggagaat
     3901 atggtgcagt tagtcgatct cgcacgttgc gtttcgtttt gattccgtct gatttcccag
     3961 ccttataagg gtgtgaatta tggagatgta ttttaaaaga atgaaagatg agtggaccgg
     4021 acttgtcgaa caggcagatc ctctcattcg tgctaaagcc gcagaaattg ccgttgcgca
     4081 tgctcattat ctgagtatcg agttttatcg aattgtccgc atcgacccgc atgccgaaga
     4141 attcttgagt aatgaacaag ttgagcggca gttgaaaagt gcgatggaac gctggattat
     4201 taacgtgctt tctgcccagg ttgacgatgt cgaaaggcta atacaaatcc agcataccgt
     4261 cgcggaagtg catgcccgca taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt
     4321 gctgaaaaaa atcctctatc cggtaatctt ctcttcggat tattctgccg cagaaaaact
     4381 tcaggtctac catttctcga ttaacagtat tgatatcgct atggaagtga tgacccgcgc
     4441 gtttaccttt agtgacagta gtgcctcaaa ggaa
//
LOCUS       DAZFRJ010000272         4438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000272 DAZFRJ010000000
VERSION     DAZFRJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.274"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..631
                     /gene="lpfA-O113"
                     /locus_tag="ACHKUN_004498"
     CDS             59..631
                     /gene="lpfA-O113"
                     /locus_tag="ACHKUN_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO6662615.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            679..1410
                     /gene="lpfB-O113"
                     /locus_tag="ACHKUN_004499"
     CDS             679..1410
                     /gene="lpfB-O113"
                     /locus_tag="ACHKUN_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO6662616.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1435..3957
                     /gene="lpfC-O113"
                     /locus_tag="ACHKUN_004500"
     CDS             1435..3957
                     /gene="lpfC-O113"
                     /locus_tag="ACHKUN_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO6662617.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3968..>4438
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUN_004501"
     CDS             3968..>4438
                     /gene="lpfD-O113"
                     /locus_tag="ACHKUN_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO6662618.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSG"
BASE COUNT         1218 a          940 c         1067 g         1213 t
ORIGIN      
        1 acttattaat ataagtgatg attcatggta aaggatatat tatatcaatg gagtttttat
       61 gaagcgtaat attataggcg gtgcattcac tctggcatct ctaatgctgg ccgggcatgc
      121 actggcagaa gatggtgttg ttcacttcgt cggtgaaatt gtcgacacta cttgtgaagt
      181 tacctccgat acagccgatc aaattgtccc actgggtaaa gtcagtaaaa atgcattttc
      241 aggtgtaggt agtctggcgt cgccacagaa gttcagtatt aagcttgaaa attgcccggc
      301 aacgtacact caagcagccg ttcgttttga tggtacagaa gcgcctggcg gcgacggcga
      361 cctgaaagtg ggtacgccgc ttacagcagg caaccctggt gattttaccg gtacaggaca
      421 agcgattgcg gcaaccggcg ttggtattcg tatttttaac cagtccgata attcgcaggt
      481 taaactttat aacgactctg cttataccgc tatcgatgct gaaggcaagg ctgaaatgaa
      541 gtttattgca cgctatgtgg caaccaatgc gaccgtaacg gctggtacgg cgaacgctga
      601 ttcacaattt actgtcgaat ataagaaata atttatcaag ggccttttta ttcagttggc
      661 tttggccagg gagtcgttat gtccagaaaa agttgtttaa tcaggctctt taaaacaaca
      721 gtttcatgta agttatttac cgcgattata ctcagcgttt ttttaggcca accagcatta
      781 acttatgcag gcgttgtcat tggtggtacg cgggtggttt acttaagtaa taatgctgat
      841 aaatctattt ctgtatttag caaagaagaa aaaatacctt atctcatcca ggcttgggta
      901 gatccattta ataaagagga taaaagcaaa gcgccgttta ccgttattcc gccagtatca
      961 cgattagaac cttcgcaaga aaaaatatta cgcattattc acaccaaagg tgtgtcattg
     1021 cctgatgatc gcgagtcagt cttctggctg aatattaaaa atattccgcc atcagcgagc
     1081 aacaaagcga cgaactcgct ggaaatagca gtaaaaacgc gtatcaagct gttctggcga
     1141 ccagcaaata tccgccttat tcccgaagat gctgcgccga aagtgaaatg gcgccgggaa
     1201 gggcgaaatc tgatagcaga gaatcccaac ccaattcata tcagtgtgat ggatgtcatc
     1261 gttgatggcc atgatgtgcc cctcaatatg atccgtcctt ttgaaacgct cacgttacct
     1321 ttacctgcta attctgcggg aggccaaatg acctggcgat ttattaatga ttacggcgca
     1381 gtcagtgacc cgataaaaat gacgctgtaa gcaagggata aggataggcg tattatgatg
     1441 acgaccagaa tagtggttgg cctcacggca gggacgtgtc tgattttctc gcaaaacctg
     1501 atggccgagg tcagtgtatt caatccggcg cttctggaaa tcgaccatca atccggagtc
     1561 gatattcgcc agtttaatcg ggcaaacctg atgcccccag gtgtttatag cgttgatatt
     1621 tttatcaacg gtaaaatgtt tgaacgtcag gatgtgacat ttgttcagga taatccagat
     1681 gctgatctgc acgcttgctt tattgccatt aaaaaaacac tgtcctcctt tggcataaaa
     1741 gttgatgcgc tcaaatcgtt caatgatgtg gatgagacgg tttgcctcga tcctgctcca
     1801 cgtattgaag gctcatcctg gcagtttgac agtgataaat tgcagctgaa tatatccatt
     1861 ccccaaatct acatggacgc gatggcttat gattacatca gccccacgcg ttgggatgag
     1921 gggattaacg cgctcaccat caactacgat ttttctggtt cacatacact acgttcagat
     1981 tatggttcac aagagacaga taccagttat ctcaatctgc gcaatggact gaatattgga
     2041 ccgtggcggc tacgtaatta tagtacttta aacaccagcg atggccgtgc ggaatacaac
     2101 tccattagta cctggataca gcgcgatatt gccgcgttaa gaagccagat tatgattggt
     2161 gatacgtgga cggcgagcga tattttcgac agtacgcaaa ttcgcggcgc gcgtttgtat
     2221 actgataacg atatgctacc cgccagccag aatggctttg ctcctgtggt tcgtgggatt
     2281 gcaaagtcca acgccaccgt catcattcgg cagaatggct acgtgattta tcagtctgcc
     2341 gttccacaag gtgcttttga gatcaccgat ctcaacaccg ccagtacagg tggcgatttg
     2401 gacgtaacca tcaaagaaga agacggtagc gaacaacgat tcacccaacc ttatgcttca
     2461 ttggcgattc ttaaacgtga aggtcagaca gatgttgatg tcagcgtggg tgaattgcgc
     2521 gatgaagacg gatttacacc ggacgtcctt caggcgcaaa tacttcatgg tttttcccac
     2581 gggatcactt tatatggagg tatgcaggct gctgaaaatt atggttctgc agctctgggt
     2641 gtcggtaaag atcttggcgc tttgggcgca atttctttcg atgtgacaca tgctcgtgcg
     2701 aattttagcc atgatgatac agaaacgggt cagtcatatc gctttctcta ttcaaaacga
     2761 tttgacgaca cagacactag cttgcgcctg gttggctatc gttactccac cgagggctac
     2821 tataccctca atgagtgggc atcgcggcgc aacagccctg aagacttttg ggaaacaggt
     2881 aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga ttatggcaat
     2941 ttatacctga cattaagccg gcaacaatac tggcataccg atgatgtcga acgattaatg
     3001 caatttggct acagcagtag ctggaagcgt ctctcgtgga acgtctcctg gagttattcc
     3061 aatactgcca gacaggggac ggggaacaac catgccagtg ataacaccag tgagcagatc
     3121 tacatgctct ctttatctgt tcctttatcg ggctggtggg gtaatagtta cgccacctat
     3181 tctgtttcgc aaaacgataa ttccggtagc tcacatcaac tcggactcag cggtacggcg
     3241 ctggaacgaa ataacctttc atggaattta atgcagtcct ataacagtca tgatgatgag
     3301 gttggcggta atatgtccct gacctatgat ggctcttatg gcacggtgaa cggcagctat
     3361 aactacagcc aaaattccca gaggctgaat tatggtatca gagggggaat tctggcacac
     3421 agcgaagggg taacgttaag tcaggagtta ggtgaaacta ttgctcttgt taaagcacct
     3481 ggggccgccg ggttagaaat agataatatg cgcggtgctg cgacggactg gcgcggctat
     3541 acggtcaaga cacagctaaa cccttatgat gaaaatcggg tagcaatcag cgataactat
     3601 ttctcgaagt cgaatataga acttgataat accgtcgtta cgatggtttc cacgcgtggt
     3661 gcagtggtta aagcggagtt tgtgactcat gtgggttatc gcgttctctt cagagtgtta
     3721 aatgcaaatg gtaaaccggt accttttgga gccattgctg cgatacaaga tgcaagtttg
     3781 gcagattcag gaattgtcgg tgaccgtggc gaactttatc tttctggtct accagaaaaa
     3841 ggacaggtta cgttatcctg gggagaaaac gcctcaacaa aatgcatctt caattattca
     3901 ctttcgacac cagaaagtga gagcggatta attgaacagg gtgtgacatg tcattaaaag
     3961 gatttttatg aacaagtata tcaaacagtg gtgctttgct gtgtttatgc tctcgttaag
     4021 tagtgtagca cttgcagctc ctaaaggtat ctgtacccct gataatggcg tatttcacag
     4081 tacgcttgat ttctcgggat atcttattac cgcgaacgaa aataaggttg gaacgacatt
     4141 taacaccact gtaactaatg gatcctcata tcctggacgc tgtcattgtg atactggtaa
     4201 cgtaggagaa ttcccctata tctattatac gtcaaaaata aaccaggcat taacttatgc
     4261 tggggttcac tctaatatta attattatga tcttaatccg aatcttgatg ttgggattgc
     4321 tatagatatt cttggggttg ggtatgtgaa tgcacctttt gaatatcatg ctaacaaccc
     4381 gtctggtaat acaaagtaca attgcaaccg cattgaacct ttaagtatat ccagtggg
//
LOCUS       DAZFRJ010000273         4404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000273 DAZFRJ010000000
VERSION     DAZFRJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.275"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(92..742)
                     /gene="zinT"
                     /locus_tag="ACHKUN_004502"
     CDS             complement(92..742)
                     /gene="zinT"
                     /locus_tag="ACHKUN_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO6662619.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(999..1634)
                     /gene="msrQ"
                     /locus_tag="ACHKUN_004503"
     CDS             complement(999..1634)
                     /gene="msrQ"
                     /locus_tag="ACHKUN_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO6662620.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(1635..2639)
                     /gene="msrP"
                     /locus_tag="ACHKUN_004504"
     CDS             complement(1635..2639)
                     /gene="msrP"
                     /locus_tag="ACHKUN_004504"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO6662621.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(2748..3161)
                     /gene="hiuH"
                     /locus_tag="ACHKUN_004505"
     CDS             complement(2748..3161)
                     /gene="hiuH"
                     /locus_tag="ACHKUN_004505"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO6662622.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3294..3965
                     /gene="hprR"
                     /locus_tag="ACHKUN_004506"
     CDS             3294..3965
                     /gene="hprR"
                     /locus_tag="ACHKUN_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO6662623.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3965..>4404
                     /gene="hprS"
                     /locus_tag="ACHKUN_004507"
     CDS             3965..>4404
                     /gene="hprS"
                     /locus_tag="ACHKUN_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6662624.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLA"
BASE COUNT         1215 a          940 c         1011 g         1238 t
ORIGIN      
        1 tctgagtttg tcttacttat ttgtcttggc aaattattgt tgttactacc aggcactgtt
       61 ctgttaaata actgaagtca gcgcaaaaaa atcaatgaga catcatctcc tcgaccactt
      121 cttcgctact caactgatag ggataatacg ttggccagtt ttccatttca ttcaataaag
      181 actgttggga atcattaccc ataaaaatgt gaaaatgaga tgattttcgt ggtgcaatta
      241 tatgatcgct aaattgtata tatttagggg ctttactttc aggatcttta cattcgaata
      301 agtaacgaac gcctttcttg cctgatttat aggtgagtat tttgtatcca tcgtaatcat
      361 atttacagga tgttgtttca ttatttctat ggaattcgac aatgccgtcc tcaatgccaa
      421 tcatctcgat gtctgtcaca taacctttgc gataataatc tttaatttca gcaaatgttt
      481 tggttttatc tgcatccgct ttcttctgaa agatggggtc aagtttgcca ctttgcagta
      541 aaggataaac ggattgccag actccatccc agtcactgag cgttcggttt tgtacattgg
      601 catcatcaaa aacaccatta gcagcttttt gttcgacctc tgttaagggt ttgccgtgtg
      661 agtgatgacc atgcgaaaag gcaggagcgc taacaataaa gacacctaaa gcaacagcca
      721 gtttatgaag acgaatcgcc aaaacagttt cctccagagt taatgtatat gatgtgtaat
      781 gttatattgt aacatataac aaaatgcaac aactcttagg agacaagaga atagttctct
      841 caggtgtgct tatctatctt attgaaataa aataaattat gtcgatgatt tgaggttgat
      901 gacaaacgaa acataacttg atgtgatctg cgtaggccgg atagggcgct cgcgccgcat
      961 ccggcaggga taacgcgtac tttgtcggca atatgaagtt atacagataa tttgttgtga
     1021 acctgcttgc gtaaccggtt aaaaagggaa agcaacttct tataccgtaa ggctaaaagc
     1081 agtaccgcca gcccagcgta gatgagtggc tgcggtgaaa taatcttcac tgaccacaaa
     1141 taatgtattg gggccaggat cgcgacaaga tagacgaagt tatgcaactg ttgccagtgt
     1201 ttaccgagtt ttcgctgcat cgcctgggtt gaagtgaacg ctaacgcaag caaaattacc
     1261 cagctgataa tacctaacgt taaataaggc cgggtaatta actccttacc taataacgcc
     1321 agattattca cgcccagctc cagcaatgcg taactggtta aatgcagtgt cgcccaggca
     1381 aagcaccata agcctaacag gcggcgagtg cgtatcaata acggctgttt tgcgtagcgt
     1441 gccagaggtg tgattaacaa ggtcgccagc aaaaatttca gtgcagtgcg accagtaaaa
     1501 tgctgaatat ctttcaccgg atcggcaccc aatccaccgt gattaatcgc ccagaccagc
     1561 cagagaaacg gcaacaatcc ggcaagatgc aggcaaactt tcagccatgt cacctgtttt
     1621 gctgtcagac gcatttagaa attctcccgc aaatccaggc cacgatacag cgatgccacc
     1681 tgctcggcgt aaccattaaa cagtagcgtt ggctggcgct gtacatcgag gatgccgcct
     1741 gaaccaataa atcgttcggt agcctgtgac cagcgcgggt gatcaacatg cggattaacg
     1801 ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc aggtggttgg cggacgctcg
     1861 cgggtcagct taatactgac tatcgattta atccctttaa agccatattt ccacggcaca
     1921 atcagtcgca ccggcgcgcc attttgtggc ggtaacgcct tgccataaac acccacggtc
     1981 atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc cttcgacata aggatatttc
     2041 agcccgccac cgataaagcg gtcctgctgg cctggcattt gttccggtgc ataaattgtt
     2101 tcgaaagcga catacttcgc attgctggtg ggttcggcaa gcgccagcaa tttgtgcagc
     2161 ggaaaaccaa tccacggcac caccatcgac catgcttcca cgcagcgcat acgataaata
     2221 cgctcttcca gcggaaagcg acgggttaaa tcatcgtgat cgagggtcaa tggttttgcc
     2281 acttcgccgc tgattttcag tgtccatgga tcggttttca ggctaccagc attagcggcg
     2341 ggatcggctt tatccagccc gaattcatag aagttgttat aaccggagac tttatctgct
     2401 ggcgtcagtg gcaggtcatt ttgccaggcg gcaggcttgc tgaactccaa cggttttccg
     2461 gcgggggcgg gtgggcgatc attccctttg aaccagctaa gcagatcggc atgcgcagcg
     2521 tgaggcaaag aaagtgcagc cgcgctgatg cccagtgctt ttaacacctg ccgacgcgtc
     2581 ataaagaata ccgactcggc cgtaacatct gattctttta aaaattgatt ctttttcata
     2641 agccatcaca cttttttgtc tttagccagc aagagccgtt tactgttaat ctgtcaggaa
     2701 gtgtcctccc taacagtagc agagatctgc ttttacatat ttatgcatta actgccacga
     2761 taggttgaat acccatattg gctaagtaat aaaggcacat gataatgctc gttcacttta
     2821 ttaatatgaa attcaacggg gatctcaggg aagaaacttt caagattttg tttcttgaaa
     2881 tagtccccgg ttttaaatac gacacggtaa tcgcccgtag ttgcagtttg ctcgggccac
     2941 agtgccttaa ttcgtccatc cttatctgtt ttggcggtat taagttgtaa ccagccgttg
     3001 tccgctttct tttcaagagt gactgtcacg tcggcagcgg gttttcctgt ttgctggttc
     3061 aaaatgtgca cactaagaat gttttgttgt gcagcataga ccaaagaagg taatgaaaat
     3121 gcagccgtta ctacggagag tactaaataa cgctttaaca tgtttatatc cttgtcatgt
     3181 gaatgagttt tgaggatagc gaaaaagcgt ccacggtaac cttacagcag cattacaatt
     3241 ttgtaatgaa ccaggctgtt tctataacat atgatttatg gcatattatt ttcatgaaga
     3301 ttttacttat tgaagataat caaaggaccc aggagtgggt aacgcagggg ctttccgaag
     3361 cgggttatgt cattgatgcc gtttctgatg gcagagatgg gctttatctt gcgcttaagg
     3421 atgattatgc attgatcatt ctggatatta tgcttccggg tatggatggc tggcagatct
     3481 tacaaacgtt aagaacagca aagcaaactc ctgttatttg ccttactgca agagattctg
     3541 tcgatgacag agtcagaggg ctggacagtg gggcaaatga ttatctggta aaaccttttt
     3601 cattttctga gttgctggca agggttcggg cacaattaag gcaacatcac gctttgaatt
     3661 caacattaga aatcagtggc ttaagaatgg actctgttag tcaaagtgtg agcagggaca
     3721 atatcagtat tacactgacg cgcaaggagt ttcagttact ttggctactg gcctccagag
     3781 ctggcgagat tatccccaga acggttattg cgagtgaaat ttggggaatc aactttgata
     3841 gtgataccaa tacggtggac gtcgccattc gcaggctccg cgcaaaagtt gatgatcctt
     3901 ttcctgaaaa gctaatcgcc acaatccggg ggatgggcta ttcattcgta gcggtaaaaa
     3961 aataatgaaa agattatcta taaccgtccg tttaaccttg ctttttatat tgctgctatc
     4021 tgttgctggc gctggaattg tatggactct ctataatggc ctggcaagtg agttgaaatg
     4081 gcgcgatgat acaacactca ttaaccggac agcgcagatc aagcagttgt taattgatgg
     4141 ggtaaatcca gatacgttac ctgtgtactt taaccggatg atggatgtta gtcaggatat
     4201 cttgatcatt catggtgata gcatcaacaa aattgttaac cggacgaatg tcagtgatga
     4261 catgttaaat aacatacctg ctactgagac aatcagcgca gctggcattt acagaagcat
     4321 tattaatgat acagagatag atgctttacg aattaatatt gatgaagttt cgccatcatt
     4381 aacggttact gtggctaaat tggc
//
LOCUS       DAZFRJ010000274         4371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000274 DAZFRJ010000000
VERSION     DAZFRJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.276"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..539
                     /locus_tag="ACHKUN_004508"
     CDS             <1..539
                     /locus_tag="ACHKUN_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6662625.1"
                     /translation="KSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDED
                     LTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTD
                     LLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSK
                     DATPGNVYAQADYKIAYE"
     gene            599..3103
                     /locus_tag="ACHKUN_004509"
     CDS             599..3103
                     /locus_tag="ACHKUN_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6662626.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3119..3868
                     /locus_tag="ACHKUN_004510"
     CDS             3119..3868
                     /locus_tag="ACHKUN_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6662627.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3870..4371
                     /locus_tag="ACHKUN_004511"
                     /pseudo
     CDS             3870..4371
                     /locus_tag="ACHKUN_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1362 a          910 c          891 g         1208 t
ORIGIN      
        1 aaaagtcact tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg
       61 aaggtcacgg cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac
      121 caggtgacga agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg
      181 accagaaatc tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg
      241 gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc
      301 agactgacct gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg
      361 tacgtattct ggatagccag agcggcgaag tgactttagg tactccggta gtaattacat
      421 tcaacaatac taacagctat caggaactga acttcaaagc tcgtatggaa tccccaagca
      481 aagatgctac cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat
      541 cacattaggc cgggttcgcc cggccacatc attaacggat taatgataag tggaacagat
      601 gtataaaaaa ttaaaattaa ccacaataag cgaattgatt aaaaatattt attgttcatt
      661 atccgttctc atcattggtt gtgcgtcagc ttatgccgtt gaattcaaca aagatttaat
      721 cgaagccgaa gatcgtgaaa acgttaacct ttcccaattt gaaactgatg gccaattacc
      781 cgtaggtaaa tactcattaa acgctcttat taataataaa aggacgccaa tccatctgga
      841 cctgcaatgg gtattaattg ataaccaaac tgcagtttgt ctgactccag agcaattaac
      901 attattaggc tttactgatg agattattga agaggctcag caaaacctga tcgatggttg
      961 ttaccctatc gaaaaagaaa aacaaattac aacttatctc gataaaggga aaatgcaatt
     1021 atccatatct gcacctcagg catggttaaa atacaaagat gcaaactgga cgcctcctga
     1081 actttgggat catggtattg ctggggcatt tcttgactac aatttatatg cctctcatta
     1141 tgcaccacat cagggcgata attcacaaaa tataagttcc tatgggcagg ctggggttaa
     1201 tcttggggcc tggcgcctgc gtactgatta ccagtacgat cagtcattta acaatggcaa
     1261 aagccaggcg aacaacctgg attttccgcg tatttatttg tttcgcccaa tcccagcaat
     1321 taatgcaaaa ctaactatag gtcaatacga tactgaatcc tctattttcg actctttcca
     1381 tttttctggc gtttcgttga aaagcgatga gaatatgttg ccgcctgatc tacgtggtta
     1441 cgcaccgcaa atcacgggtg ttgcacaaac gaatgcaaag gtcactgtct cacagaacaa
     1501 ccgtattatt tatcaagaaa atgttcctcc tggcccattt gctattacta atttattcaa
     1561 tacattacag gggcaactgg acgtcaaggt tgaagaagag gacgggcaag tcacgcaatg
     1621 gcaagttgca tctaatagta ttccttatct gacgcgtaaa gggcagattc gctacaccac
     1681 tgctatgggt aaaccgacca gcgttggtgg tgattcctta caacaaccct tcttctggac
     1741 tggtgaattc tcatggggtt ggctgaacaa tgtatccctg tatggtggtt cagttttaac
     1801 aaaccgtgat tatcaatctc tggctgccgg cgttggcttt aaccttaact cattgggttc
     1861 attatctttt gatgtcacac gatctgatgc tcagttgcat aatcaggata aagaaacggg
     1921 ttatagctac cgcgctaact attcaaaacg ttttgaatct accggtagcc agctcacttt
     1981 cgctggttac cgtttctctg ataaaaactt tgtgacaatg aatgaatata tcaatgacac
     2041 taaccattac acgaattatc agaatgaaaa agagagttat attgtcacgt ttaaccagta
     2101 tcttgaatca ttaaggttaa atacatacgt aagtttggct cgtaatactt actgggacgc
     2161 cagcagtaat gtgaattatt cattatcact tagccgcgat tttgatatcg ggccattaaa
     2221 aaacgtctcc acttctctaa catttagccg aataaactgg gaagaagaca accaggatca
     2281 actgtaccta aatatttcga ttccctgggg aaccagtaga acattgagct atggtatgca
     2341 acgaaatcag gataacaaga tttcgcatac tgcttcgtgg tatgactctt ccgatcgaaa
     2401 taattcctgg agcgtttctg cttcaggcga caatgatgaa ttcaaagata tgaaagcgtc
     2461 actacgcgcc agttatcagc ataataccga gaacggtcgt ctctatctct ccgggacatc
     2521 acagcgagac agttattatt ctctgaatgc cagttggaat ggttcattca cggcgactcg
     2581 tcacggagcc gctttccatg actatagtgg tagtgctgac tcgcgtttta tgatcgacgc
     2641 agatggtgca gaagatattc cgttgaacaa taaacgcgcg gtaactaatc ggtatggcat
     2701 cggagttatt ccatcagtca gcagttacat aacaacatca ttaagcgttg atacccgaaa
     2761 tcttccagaa aatgtggata tcgaaaactc ggttatcacc accaccttaa ccgaaggtgc
     2821 tattggctac gccaaacttg atacccgcaa gggctatcaa atcatggggg ttattcgcct
     2881 ggcagatggt agtcacccac cactggggat tagcgtaaaa gataaaacca gccataaaga
     2941 attaggactg gttgctgatg gcggctttgt atacctcaac ggcattcagg atgacagcaa
     3001 acttacttta cgctggggtg ataaatcctg ttttattcaa ccacccaata gcagcaactt
     3061 aaccaccgga acggttattt taccgtgtat tagccaaaat taattcagga tgaaactaat
     3121 gcgctactta aacacaaaga atctcattgc agcaggagta ttactcgcct gcatgagcag
     3181 cattgcctgg ggtgccatca tcccggaccg tacccgtatc ataatgaatg agtcagataa
     3241 aggggaagca ttaaaactta ctaatcaaag caaaaatcta ccctatctgg cgcaaacatg
     3301 gatagaagat acgaaaggca ataaatcacg tgactttatt gtcacggttc cccctatggt
     3361 acgtttaaat cccagtgagc aaattcaaat aagaatgata agtcaggaaa aaattgctca
     3421 actgcctaat gacagagaaa cgctcttcta ttttaacgtg cgagaaatac cgccaaaaac
     3481 agataaaaaa aatgtgatgc aagtcactat gcagcacgca ttgaaactat tctggcgacc
     3541 aaaagctatt gaattagaag atgatggggt aatgtcttac gaaaaagttg aaatcatccg
     3601 tagaaatgat ggttcgatcc gctttaacaa taagatgcct tatcacgtta cgctgggtta
     3661 tatcggaaca aacggaatca caatgttgcc acaaacgcaa agtttaatgg taacccctta
     3721 tagccatgca gatacgcagt ttaaaaatgt tccgtcagct tttcaggttg gttatatcaa
     3781 tgattttggt ggattaagtt tttatgaaat aaactgcccg acggttaata actcttgtaa
     3841 tgtttccgta gccaaacgag ataaataata tgcgctactt attaattgta attatattta
     3901 ttataggttt tagtgcatta cctgtatggg caatggactg ctatgctgaa catgaagggg
     3961 gaaataccgt agtcattggt tacgtaccaa gaatttctat ccccagtaat ggtaaaaaag
     4021 gtgataagat ctggcaaagc agtgagtatt ttatgaatgt cttctgtaat aatgcattac
     4081 ctgccccatc acctggagaa gaatacccat cagcctggac taatataatg atgtttttac
     4141 caggcggtca ggacttttat aatcagaact cctatatttt tggcgtaaca tacaatggag
     4201 ttgattatga ttcgactgcc cctaatgcta tcgcagcacc agaatgcatt gatataaaag
     4261 gagcgggtac ctttaataat cactataaaa aaccccgctg tctgtagtgg aggtcctgag
     4321 cctcaattat cagtaacctt tccagctcgc gtacaactct atattaaatt a
//
LOCUS       DAZFRJ010000275         4335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000275 DAZFRJ010000000
VERSION     DAZFRJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.277"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            334..594
                     /locus_tag="ACHKUN_004512"
     CDS             334..594
                     /locus_tag="ACHKUN_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6662628.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            591..1139
                     /locus_tag="ACHKUN_004513"
     CDS             591..1139
                     /locus_tag="ACHKUN_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6662629.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1139..1363
                     /locus_tag="ACHKUN_004514"
     CDS             1139..1363
                     /locus_tag="ACHKUN_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662630.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1360..1683
                     /locus_tag="ACHKUN_004515"
     CDS             1360..1683
                     /locus_tag="ACHKUN_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO6662631.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1698..4031
                     /locus_tag="ACHKUN_004516"
     CDS             1698..4031
                     /locus_tag="ACHKUN_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO6662632.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESSRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
BASE COUNT         1040 a         1019 c         1131 g         1145 t
ORIGIN      
        1 ttttggccgt tttggctctt ttattaatca ggtgttgtct tagtgcttat gatgtaacgc
       61 cctgaatgat atgggctggt tcagatttga gctgtgatcc tgcctagtgt aatagacttt
      121 tttattattg atgctcctta ttatattcca taaaggaatt gtttgttttt gttcgtttca
      181 tacggttttt agctgtttta ttttttgaga aatcaacatt tcacacaaaa taaataaagc
      241 atgcgcgagc cgcttatata aacataataa ggagctacct gaatccgcat ggaattgccc
      301 ggactgttgt aaacatatta gagaggtagc cagatgaata gtatttccgt ttccaccccg
      361 gcaccagctg ctccggtgct cccggttccg gctcagcagg agcgcttcat acgattacct
      421 gaagtgatgc acctttgcgg gctatcccgt tcaaccatct acgaccttat cagccgcgat
      481 gccttcccgc agcagatatc cctgggtgga aaaaatgtgg cctgggtaca gtcggaggtc
      541 agtgcatgga tggcggcgcg aattgctgcc cgcgagcaag gaggccatgc gtgaaacttg
      601 ctttgcagtc tcatcttctt ttttctggct tgcgtcctga tatcttttcc aggtatagtt
      661 ttcccgctgc cgcaaaatcg gctgccgggc gtaggaaccc gagttactta ttggcgacac
      721 cagacgcgcc gtgcgtcttt ttttacgtcg ttgctcaggc acacccattt ttcgggctgc
      781 ggtgcttata ccttagcccc tttcagataa tggtggtccg ggcggggcag ccttcgggct
      841 ggccggtttc caataaggcc ggtattccta cccccgttcg ggtcaccacc catgagcgta
      901 ggaactccgg tggtggcatt taccgctact tattggaggt tgcccttatg gctacgatcc
      961 tcgccccgtc acatcctcaa tttatatttg tttttgccgc cgttcgtcgt gcagaacgta
     1021 aaccacgtat cagtatgctg cgtatcatcg ccagcaatga gcatcatgca cgtctttcac
     1081 tggtccatga gtatgtgctt tgtcttgctg ctcgcctgcc tgtccgggag gtgatctaat
     1141 gaatcacgcc gcaatctctt atgacgatat tctccgcctg aagcatcttc gtaacgtggg
     1201 agagtttgtc actggaatgg ctgttcttca ggattgctac gaaaaacctg ccagtgctca
     1261 atgtgaacaa ctggtttccc tgatttatct gatgacagag cagcttgatg gcgtggtaca
     1321 gcgttgccag gatgacctca tgaacatgga ggtggtgcaa tgaaatcacg cactctttcc
     1381 ctttcacttc gggtagcgtt gtatcgccgc gcggtagcct gcgcctggtt gagtgcctgc
     1441 tatcagcaaa accgccatct gcagctcact ctggatgaaa ttgaaaccgc tattgcccgt
     1501 gagcttgaag gattttatct gcgacagcac gggcaaacaa aaggcatgga aatcgcctgc
     1561 gccctgcttt cagacctgat ggaatacggc cctctaatgt cctgtcccgc actctcccag
     1621 ctcggaatag ccgtaatgga tgagttatgc gtccgtcaca tcaaaaagcc ggttttacac
     1681 tgagggagaa ctgcgtcatg tcaggaatga aagtcagtca ggccgtgaag gctgcccgcg
     1741 gtcactgggc tcagattctg ccagcgctgg gcgtgaatat attgaaaaat cgccatcagc
     1801 cttgcccggt ctgtggcggt aaagatcgtt ttcgcttcga cgatcaggaa ggacgcggaa
     1861 catggttctg caatcagtgc ggagccgggg atggtttagc gcttgtcacc agggcactga
     1921 atgtggatat cagtgaagca gctgacagga tacatggact gacacatgat ctgtctgtag
     1981 ttaattccga ggtcaggacg ttaaccgccg ataccgatag cgggaaagat gcagcggcag
     2041 cacttgccgc gcgactgctg caagcctcgc gtgaatctgc cggaaacact tatcttacac
     2101 ataaaggttt cccggagcat atttgccatg agctgacttc agctcataag gccggtggta
     2161 tgatgttccg ccccggtgat ttgatcgtcc cactctataa cgctgacggg gagctggtga
     2221 atattcagct catcagtggc aatggtagca agtgttttct taaaggaggt caggttaagg
     2281 aggcctatca tctgatagaa gggggcggga gttctgtaag aaggatttgg attgcggaag
     2341 gttacgccac ggcgcttacc attcattatt tgacaggaga agccgtcatg gtggcatttt
     2401 cgtcggtcaa ctttctttct ctgagcagcg ttgcccataa taagtatccg ggatatcagt
     2461 taattattgc agcggaccgc gatctgaatg gttcaggcca gaacagggct gaaactgctg
     2521 caaaagcgag tcagtgtgac attgtgctgc caccggtttt tggtgactgg aatgatgcgc
     2581 ttacccaata cggagaggaa tccacccgga aggcaattct tgaggctttg aagccacata
     2641 acgccagtcc tttcgacaca atgagtgaag cagaattcac agcgatgagt gtcagcgaaa
     2701 aagctcagag ggttcgggag cattacaggg atgcactggc ggttgatccg aacgggcagc
     2761 ttttatcccg ctacgagtct ggagcgtgga aagttatttc tcagtcagat tttgcccgag
     2821 atgtcgcagc ccttttccag cgcctcgggg caccgttttc ctcaggaaaa attgcctcac
     2881 tggtggaaac attaaagtta attgttccgc agcagcaaaa tccgtcacgt catctgattg
     2941 gttttcgtaa tggcgttctc gatacgcgaa cagggctttt tagtccgcac tgtaaagaga
     3001 actggctgcg taccctgtgt gaagtcgatt tcacgccacc ggtaaagggg gaaacgcttg
     3061 aaactcatgc cccggcattc tggcgttggc tggaccgggc cgccggacac aagccagcaa
     3121 aacgcgacat tattcttgca gcgttattta tggtgctggc gaaccgctat gactggcagc
     3181 tctttctgga agtgactggc cctggtggaa gtggcaaaag tattctggct gaaattgcga
     3241 ccatgcttgc tggagaggat aacgccacct ctgcgaccat tgaaacgctg gagtcctcgc
     3301 gagaacgtgc ggctctgata ggtttttctc ttattcgcct gcctgatcag gagaagtgga
     3361 gcggcgacgg cgccgggctt aaggctataa ctggtggcga tgcagtatct gtggacccca
     3421 aatatcagaa tgcttattca acacatattc cggcggttat cttggccgta aataataatc
     3481 ctatgcgctt cactgatcgc agtggtggtg tgtcccgcag gagagttatt ctgcattttc
     3541 ctgagcagat agcaccggaa gaacgcgatc cgcagttgaa agataaaatt gcacgagaac
     3601 tggctgtgat tgttcgtcag ctcatgcagc gtttcagcga tccgatgagc gccagaacat
     3661 tgcttcagtc gcagcagaat tctgatgagg ctctcaccat caagcgtgat gctgatccag
     3721 catttgattt ttgtggctac cttgaggcat tacctgacac caacggtatg tttatgggga
     3781 acgccaatat tgtcccacgc cagccacgta cataccttta ccatgcctac ctcgtataca
     3841 tggaggctaa tggctataaa aacaccctga gtcttacgat gtttggcaaa gggttgccag
     3901 tcatgctgaa ggagtacggg ctacattatg agaaacggcg aactaatcag ggaatgcaaa
     3961 ctaacctcac tttgaaagag gagagtaatg cagactggct gcccaagtgt gatgagccaa
     4021 tattaaaata acgattaact aacccggctg atgccgggct tcttattgga aacgcacaaa
     4081 tgtagagata gctgttcact cttcacctcg ctgttaacac gtaagcaatt gatatataag
     4141 ttaaaaatgg cagggtgaat agtgtgcatg gttttgcgaa aaaaaagtat tttttgtaga
     4201 gtcaagtccc ggtttggatc cgtttaacgg aacctaacta gattgactat ttctctgatg
     4261 atttttggat aaataatcaa caaaaatcaa aatgtgaaca gagaatggtg ggggcacaaa
     4321 aaggggcata attga
//
LOCUS       DAZFRJ010000276         4331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000276 DAZFRJ010000000
VERSION     DAZFRJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.278"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            261..2369
                     /locus_tag="ACHKUN_004517"
     CDS             261..2369
                     /locus_tag="ACHKUN_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6662633.1"
                     /translation="MSTGLRFTLEVDGLPPNAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKPPDEKQDTPDMREY"
     gene            2437..>4331
                     /locus_tag="ACHKUN_004518"
     CDS             2437..>4331
                     /locus_tag="ACHKUN_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO6662634.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAV"
BASE COUNT          984 a         1175 c         1355 g          817 t
ORIGIN      
        1 tcaggttttt tattactcgc tgtaatgtac gagcctgtcg tgaataacgt cgagaaccca
       61 tccgttgcgt ttgtgattat tttgttgact aaacagacac ccgtttctct gaagtaaaat
      121 cccagactaa atcatcacat aaccatgaca tttttctgat attccccggt aacgccagat
      181 gtcgactcgc ttatctcatc cgcccgacat gttgtacatg caatatgtgc ggattattat
      241 tcattttcac ggaggttgct atgtcaaccg gattacgttt cacactggaa gtggacggcc
      301 tgccgccgaa tgcttttgcg gtggtttcct ttcatctgaa ccagtcactc tcttcgctct
      361 tttccctcga tctctccctg gtcagccagc agtttctctc ccttgaattt gcgcaggtgc
      421 tggacaaaat ggcctacctg acgatatggc agggcgatga agtacagcgc cgggtgaaag
      481 gcgtggtgac ctggtttgaa ctcggggaga acgacaaaaa ccagatgctg tacagcatga
      541 aggtgcaccc gccgctgtgg cgtgccggtc tgcgccagaa cttccgtatc ttccagaacg
      601 aggacatcaa aagcatcctc ggcacgatgt tgcaggaaaa cggggtgacc gaatggagtc
      661 cgctgttcag cgagccgcat ccttcccgtg agttttgtgt ccagtacggt gagactgatt
      721 acgatttcct gtgccggatg gcggcggagg aaggcatctt cttttatgag gagcatgctt
      781 acaaaagtac cgaccagagc ctggtgctgt gcgacacagt ccgccatctg cccgaatctt
      841 ttgaaatccc atggaacccg aacacccgta ccgaggtgag caccctctgc atcagccagt
      901 tccgctacag cgcacaaatc cgcccttctt ccgtggtgac caaagactac acctttaaac
      961 gccccggctg ggccggacgt tttgatcagg aaggccagca ccaggattac cagcgcacac
     1021 agtatgaagt gtatgactac cccggacgtt tcaagggggc ccacgggcag aactttgccc
     1081 gctggcagat ggacggctgg cgaaacaatg cagaaaccgc gcggggaatg agtcgctcgc
     1141 cggagatatg gccgggacga cgaattgtgc tgacggggca tccgcaggcg aacctgaacc
     1201 gggaatggca ggtggtggca agtgaactgc acggcgaaca gccacaggcg gtgccaggac
     1261 ggcagggagc ggggacggcg ctggagaacc attttgcggt gatcccggca gacagaacat
     1321 ggcgaccaca gccgttgctg aaaccgctgg tcgacggccc gcagagcgct gtcgtgacag
     1381 gaccggcagg cgaggaaatc ttctgcgacg aacatggtcg cgtgcgggtg aagttcaact
     1441 gggaccgtta taacccggca gaccaagaca gttcgtgctg gatccgtgtg gcacaggcgt
     1501 gggcaggcac cggttttggc cacctggcga taccgcgtgt gggtcaggag gtgattgtgg
     1561 acttcctcaa cggcgacccg gaccagccga ttattatggg gcgtacctac caccatgaaa
     1621 accgcacacc cggcagcctg ccggggacga agacgcagat gacgatccgc tcgaaaacct
     1681 ataagggcag cgggtttaat gaactgaagt ttgacgatgc gacagggaaa gaacaggtct
     1741 acatccacgc gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga
     1801 taaacaacca tgctgagacc attggcaaca atcagatgat tgcggttacc aacaatcaga
     1861 tacagacggt gggcgttaac cagatagaga cggtgggcag taaccagatc attaacgtgg
     1921 gttctgttca ggtggaaacg attggacttg ttcgtgcgct gaccgtgggc gtggcgtacc
     1981 agacgacggt aggtggcatt atgaacactt cggtggctct gatgcagtcc tcgcagatcg
     2041 gtttgcataa atcgttgagg gtcgggctgg gttatgacgt caaagtcgga aataacgtta
     2101 ccttcaccgt tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt tactccgccg
     2161 gtgagcatct ggagctctgc tgtggtaagg caaggctggt gctgacgaag gacggacaaa
     2221 tttttctcaa cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac
     2281 tgttgattaa ctggaactgt gcagcctcga aatccccacc gaagccccct gatgaaaagc
     2341 aggatacgcc ggatatgaga gagtactgat cccgtacctg ttcggaattc agtaacaaac
     2401 cagcattaaa aataaacaga agaaggaatt tgaatcatga gcggaaaacc agcggcgcgt
     2461 cagggagata tgactcagta tggcggtccc attgtccagg gttcggcagg tgtaagaatt
     2521 ggcgcgccaa ctggcgtggc gtgctcggtg tgtccgggcg ggatgacctc gggcaacccg
     2581 gtgaacccgc tgctcggtgc aaaagtacag cccggcgaga cggaccttgc gctgcccggc
     2641 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggacgaagac gcctgcaccg
     2701 gtgggcgttt tcggccccgg ctggaaagcg ccttctgata tccgcttaca gatacgcgat
     2761 gatgcactga tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg
     2821 ggggaggcgg tgtacagccg cagtgagtca atgtggctgg tgcgtggcgg taaggcagcg
     2881 cagccggacg gccacacgct ggcacggctg tgggcgtcgc tgccaccgga tatccggtta
     2941 agcccgcatc tttacctggc gaccaacagc gcacaggggc cgtggtggat actggggtgg
     3001 tctgagcggg tgccgggtgc tgaggactta ctgccagcgc cgctgccgcc gtaccgggtg
     3061 cttaccggga tggcggaccg cttcgggcgg acgctggcgt accggcgtga ggccgccggt
     3121 gacctggccg gggaaatcac cggcgtgacg gacggtgccg ggcgggagtt ccgtctggta
     3181 ctgaccacac aggcacagcg ggcggaagag gcccgtaaac cgcacaccgc ttcgttatct
     3241 tcccctgaca gtccccgccc tctatcagct ccttccttcc ccgacacact gcccggtacg
     3301 gaatacggcg ctgacagcgg tatccgtctt tcggcggtgt ggctgatgca cgacccggaa
     3361 tacccggaaa atttacctgc cgcgccactg gtgtgctacg actggacgcc acgcggtgag
     3421 ctggcggcgg tgtatgaccg cagcggtacg cagatgcgcc actttactta cgatgataaa
     3481 taccggggcc ggatggtggg gcaccgttat gcaggcaggc cggagatgcg ctaccgctac
     3541 gacgatgcgg ggcgggtggt ggagcaactt aacccggcag gcctgagtta ccgctaccag
     3601 tatgagcagg accgcatcac cgtcacggac agcctgaacc ggcgtgaggt gctgcataca
     3661 gaaggcgggg ccgggctgaa gcgggtggtg aaaaaagaac tggcggacgg cagcgtcacg
     3721 cacagcgggt atgacgcggc aggaaggctc acggcgcaga cggacgcggc gggacggcgg
     3781 acagagtacg gtctgaatgt ggtgtccggc gatatcacgg acatcaccac gccggacggg
     3841 cgggagacga aattttacta taacgacggg aaccagctga cggcggtggt gtccccggac
     3901 gggctggaga gccgccggga atatgatgaa ccgggcaggc tggtatcgga gacatcgcgc
     3961 agcggggaga cagtacgcta ccgctacgat gacgcgcaca gtgagttacc ggcgacgaca
     4021 acggatgcga cgggcagcac ccggcagatg acgtggagcc gctacgggca gttgctggcg
     4081 ttcactgact gctcgggcta ccagacccgc tatgaatacg accgcttcgg ccagatgacg
     4141 gcggttcacc gcgaggaagg tatcagcctt taccgccact atgacaaccg tggccggtta
     4201 acctcggtga aagacgcaca gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac
     4261 ctgactgccg ttatcacccc ggacggcaac cggagcgaga cacagtacga tgcgtgggga
     4321 aaggcggtca g
//
LOCUS       DAZFRJ010000277         4286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000277 DAZFRJ010000000
VERSION     DAZFRJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.279"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..299
                     /locus_tag="ACHKUN_004519"
     CDS             3..299
                     /locus_tag="ACHKUN_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004905726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO6662635.1"
                     /translation="MISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQYR
                     TASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            302..661
                     /locus_tag="ACHKUN_004520"
     CDS             302..661
                     /locus_tag="ACHKUN_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662636.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(708..1100)
                     /locus_tag="ACHKUN_004521"
     CDS             complement(708..1100)
                     /locus_tag="ACHKUN_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662637.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1227..1487
                     /locus_tag="ACHKUN_004522"
     CDS             1227..1487
                     /locus_tag="ACHKUN_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6662638.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1484..1921
                     /locus_tag="ACHKUN_004523"
     CDS             1484..1921
                     /locus_tag="ACHKUN_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6662639.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2008..3018
                     /locus_tag="ACHKUN_004524"
     CDS             2008..3018
                     /locus_tag="ACHKUN_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO6662640.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3011..3472
                     /locus_tag="ACHKUN_004525"
     CDS             3011..3472
                     /locus_tag="ACHKUN_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6662641.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            3506..4231
                     /locus_tag="ACHKUN_004526"
     CDS             3506..4231
                     /locus_tag="ACHKUN_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6662642.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
BASE COUNT         1195 a          927 c         1167 g          997 t
ORIGIN      
        1 agattatctc agttaaaaca ctcagggatt tttgggcgga gaatcctgat gcagaacaac
       61 cgctaagggc atgggtggat gaggcggcaa aagctgactg gaaaagccca gcagacatta
      121 aggcacaata ccgaacggcc agtatattga aaaaccggcg cgtggtattc aacataaaag
      181 gtaatcatta ccgtttaatt gttgcaattg cgtatcagcg aggatgggta tttgttaaat
      241 tcatcggaaa ccacaaggaa tacgatgcta ttgatgctga taccatcgaa ctggagtaag
      301 catgaacatc aaacctattc gtacagagca agattatgaa gccgcgttgc gtgctgttga
      361 acccatgttc gacaatgagc ccgaaattga cacgcctgag ggggattttt ttgaggtgat
      421 gtgtttgcta atagaggaat atgaaaaaaa acattatccc attgagccac catccccaat
      481 tgaagctata agatttcgca tggaacagca ggggctgact gtgaaagatt tggaacccgc
      541 aattgggaaa aaaaatcggg tttatgaggt gttgaatggt accagaagcc ttacgttacc
      601 aatgattcgc cgtcttcata atcaatttgg tatccccctg gaaagcctgg ttggattata
      661 aaatctgcta gtcatttgcc tgatgctcgt tccagaaaag gaatgcatca ggcagttttg
      721 tttttctgcc gcagtaactc ttcaagtttc cgtttataga aatcgcgttt ttgctccata
      781 tcacgaatga tctgctctgc gtcgctttga ggtaactcat ctaaaagcga tatgattttt
      841 cgttgttgtt ctgtaagttg cggttggttg tcattactgg atacagccat tttatcgccg
      901 agagtttctt cttccataaa gaaccaatgg acgggatgtt gtgagagctc tgctaatttt
      961 tccagtttat ccattcttgg catcacgcct ttcaaccaac cttgcacgga ttggggtttt
     1021 acaccaagac gtcttcccag ctctgactgg tttatattca attcctgcaa cacctgctga
     1081 aggcgtttta caaagatcat caccacccct cgtaaactag ttccgcgatc ctacagaaaa
     1141 aattgataag tggcattaca aatagaagtt gaaatttaaa tttaaatttg taattatcgg
     1201 tgtcatcgta aagttcggag ggaaacatgc aaaaaagtac tcaagtgaaa atcctgtcaa
     1261 taatgagcca atcagaatta gggcgtcgtc ttggtaaaac accgcaaacc ataagtgggt
     1321 ggtttaaaaa acgagtgcca gcggaggagg ttataccagc atgtgaggcg cttgactggg
     1381 gagtaactcc gcatgaattg cgccctgata aataccctaa tccaaccgat ggtttacctg
     1441 ttgagtatca ggctaacgca caagcagcgg cgggagttga ttcatgaaaa tcaagcatga
     1501 gcacatccgc atggcgatga atgcgtggct gctttatccg agggtagggc gcaaaaaaat
     1561 cgctgatgat atagcgacag catattttga gcttgaaatg acttatccac caatgcatga
     1621 cacctctacg acagagggta ttggattgaa catacaaaat atttttcgct ggcttgaaaa
     1681 ggatacgcct gatgctgttg aaaaaattca ggcactaatt ccagctatcc tgactgttct
     1741 tccgcgtgaa ctgcgttatc acctcagtat ttttgacact gttgagcgcc gtgcattact
     1801 ggcggcccag gaagcgttga gcacggcaat tgatgcgcat gatgatgcag tccaggcagt
     1861 ttaccggaaa gcgtatttca gcgacggcgg gtcatccggc gagtctgttg tggtgcattg
     1921 atatttatgc cagacccctg ctgattctgt tgattgggga atcacagaat atcaccagag
     1981 gatggttcgt cacaagatga ggcaattatg gccgcattac catacatgca actgtacata
     2041 gctgattacc tggctgacac catgcatttg tccgcagagg agcacggtgc gtatttgttg
     2101 ctgatgttca attactggca aacagggaaa ccaataccta aaaacaggct ggcaaaaatt
     2161 tcccgtctga ctaacgagcg atgggctgat gtggaaccat ccttgcggga gtttttttgc
     2221 gataacagcg atgaatgggt gcatcttcgg attgaggaag atctggcatc agtcagggaa
     2281 aaattgacca aaaaatcagc cgccggaaaa gcatctgttc aggccagaag aagcagaaag
     2341 gaagcatatg ttcaaacaaa acaagagaga gatttaacag gtgttcaaac aaatgttggt
     2401 gttgtgtttg aacatgacgc aaacacaaaa gcaactaata aagatacaga tctaaaagaa
     2461 ttaaacccca cacataacgt gcgtgaacgc gagagtattc cgaccagtga gtcgcatggt
     2521 gcgccgttgc agacagccga acctgaatac ctggacggcc tgagcgaacc gatcgggaaa
     2581 ttttcgatga ctactgtctg gcagccgtcg ccggattttc gacaacgggc agcagtgtgg
     2641 ggtatggctc tgcctgagcc ggaatttaca cctgctgagc ttgccgcatt ccgggattac
     2701 tggatggcgg aggggaaggt tttcacgcag gttcagtggg agcagaaatt tgcccgccac
     2761 gtgcagcacg tcagggcaca ggtaaaacca gtcagcaagg gggtaagcca tgcagcatca
     2821 ggtggcacgg catcacgggc agttcaggaa atccgggcag cacgcgaaca gtgggaacgt
     2881 gaaaacggat ttatcagcaa cggaaacggc ctggaagctg tgggagctta tgggggaggt
     2941 gtattcgaac cgctggactc agaagaacgg ggccgcacct tcgaagctct ggattgccca
     3001 gattggtgcg atgactgaac agcaaatccg tctggtctgc cgtcagtgca tggaccgctg
     3061 ccgggcgggt gaaacgtggc ccccggacct ggctgagttt gttgcgctga tttcggagag
     3121 tggggcaaat ccatttggtc ttacggtgga tgccgtgatg gaagagtacc ggcgctggcg
     3181 caatgaatcc tggcgatacg acgggagtga taaatacccg tggccacagc ctgtgctgta
     3241 ccacatctgc ctcgaaatgc gtaccagagg gattgagcgc cagatgacgc agggtgagtt
     3301 aaaactactt gcagaacggc aactgacgaa atgggcaaag catgttggta acgggatgag
     3361 tgttccgcca gtgcgacgac aactggaagg ggcgaaacac ccgcaagggc caacgccaat
     3421 tgagcggctg aaacaggaat acgaacgccg gaaggcagct ggttttattt gaatctgaga
     3481 aacgattttg tcggaggaaa ttttaatgga aaccgtattt gacgcactga aagcactgaa
     3541 aagagcctct tcacaggtag tggcggcccg ccttggaatc agccgtgaag atgcggtcaa
     3601 cgaactgtgg aaactgaagc gccgcggtga agcggataac aaggggtcga tgtggtggct
     3661 gattcaggct ggtgaaagtg aaccggtgtc accggtaccg aaagtgacag cgcaaatgct
     3721 gactgaggcg attgaacaac atggcccaca aacggcggat gagctggcac tgatgttcgg
     3781 gattacctcc cgccgggcga attcatcgct ggccatggta atcagcaaag ggcgtctgat
     3841 tcgcgtgaat cagggcggta aatttcgtta ctgcataccg ggcgctgatt taccggcaga
     3901 gccggaagct gcatccgtag cggaaaccga tggtaaagcc tttcctcagc cagcaggtgt
     3961 tgcgttacca gttcgggaag cagaaacaca ggaagaaata aaaacggaaa gtgtggcggt
     4021 cacagtgcag tcacagccgt cgttcaccag aaaacatccg gatggtctga ttttaccatc
     4081 gctgcatgtg gctaaccgcg agctgcgccg ggcaaaaggt caggttcaga agtgggagcg
     4141 agtctgcgcc gcgctgcggg agctgaacaa gcaccgggat attgttcgac agattactga
     4201 ttcttcccgc cgtgttgtat cggaaaagtg attgccggag gcgcttatgg caaaagaatt
     4261 tacaccagaa gagcgggaag aagtga
//
LOCUS       DAZFRJ010000278         4284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000278 DAZFRJ010000000
VERSION     DAZFRJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.280"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            381..989
                     /locus_tag="ACHKUN_004527"
     CDS             381..989
                     /locus_tag="ACHKUN_004527"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO6662643.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1087..2478
                     /gene="selA"
                     /locus_tag="ACHKUN_004528"
     CDS             1087..2478
                     /gene="selA"
                     /locus_tag="ACHKUN_004528"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO6662644.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2475..>4284
                     /gene="selB"
                     /locus_tag="ACHKUN_004529"
     CDS             2475..>4284
                     /gene="selB"
                     /locus_tag="ACHKUN_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO6662645.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
BASE COUNT          955 a         1099 c         1260 g          970 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt
      181 tgtctgcttc caggcaaaac cctacagatt tataagaaag ccttaagtga gatatccatc
      241 agatagaaac aattaggaaa atatttatat caccatgata aatatgtgat ttatttggaa
      301 gtaacaaacc atcaacattt tctgcccttt ttcccatcac caggccatac tgatattttt
      361 cctcgcacaa aggactcggc atgaaactcg tcggtagcta caccagcccg tttgtacgca
      421 aactttctat tctgttgtta gaaaagggca taactttcga atttattaat gaactgccct
      481 ataacgcgga caacggcgtg gcgcaattta acccgttagg aaaagtgccg gtgctggtga
      541 ccgaagaggg cgaatgttgg tttgattcgc cgatcatcgc tgaatatatt gaattaatga
      601 atgtcgctcc ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg cgcaaaattg
      661 aggcactggc ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa caggcgcgtc
      721 cagcggcgca gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc aaccgcagtc
      781 tggatgtgct ggaaggatat ctggtcgatg gcacactcaa aaccgatacg gtcaatctgg
      841 cgactatcgc cattgcctgt gccgtcggat atctcaattt ccgccgcgtt gcgccaggct
      901 ggtgtgtcga tcgcccacac ttagtcaaac tggtcgaaaa cctgtttagc cgcgaaagtt
      961 ttgctcgcac cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac ccatctcacg
     1021 ttacaatccg tggttatgtt aaacgccctt ctccgtgtga gagggccttg atcagccagg
     1081 tttcctatga caaccgaaac gcgttccctc tatagtcaac ttccggctat tgatcgctta
     1141 ttgcgcgata gctccttcct ttctttgcgt gatacttatg gtcacacccg cgtggtggaa
     1201 ttgttgcgtc agatgctcga cgaagcgcga gaagtgattc gtggcagcca gacgctgcct
     1261 gcgtggtgtg aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga agcgcagagc
     1321 gcgctgcgtc cggtgatcaa cctgacggga accgtgctgc ataccaacct tgggcgagct
     1381 ttacaggcgg aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc agtgaccctc
     1441 gagtacgatc tggacgacgc tggacgcgga catcgcgatc gtgcgctggc gcagttgctg
     1501 tgccgcatta cgggggcgga agatgcctgt atcgttaata acaatgcggc ggcggtgtta
     1561 ttgatgttgg cggccactgc cagcgggaaa gaggtggtgg tttctcgcgg cgaactggtg
     1621 gagattggcg gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg caccctacac
     1681 gaagtaggga ccaccaaccg cacgcacgcg aatgattatt gtcaggcggt gaatgaaaat
     1741 accgcactgt tgatgaaagt acataccagt aactacagca ttcaggggtt caccaaagcg
     1801 atagatgaag cggaactggt ggcgctcggc aaagagctgg atgttcccgt agtgactgat
     1861 ttaggcagtg gctcgctggt cgatcttagc cagtacggtt tgccgaaaga gccaatgccg
     1921 caggagttga ttgcggcggg cgtcagtctg gtgagtttct ccggcgacaa gttgttaggc
     1981 gggccgcagg caggaattat tgttggtaaa aaagagatga tcgcccgcct gcaaagccac
     2041 ccgctgaagc gtgcattacg cgcggataaa atgaccctcg cggcgctgga agccacgttg
     2101 cgtctttatt tacaccctga agctctgagt gaaaaattac cgaccctgcg cctgcttacc
     2161 cgcagcgcag aggtcattca aatccaggca caacgtttac aggcccccct tgccgcacat
     2221 tacggcgcgg agtttgcggt acaggttatg ccatgtcttt cgcagattgg cagtggttcg
     2281 ctgccggttg atcgcctgcc gagcgcggca ttaacgttta caccccatga tggacgcggt
     2341 agccaccttg agtcattagc cgcccgctgg cgtgaattgc cagtgccggt gattggtcgt
     2401 atttatgacg gacgattgtg gctggattta cgctgccttg aagatgagca acggtttttg
     2461 gagatgttgt tgaaatgatt attgcgactg ccggacacgt tgaccacggc aaaacaacct
     2521 tattgcaggc gattactggc gtaaatgctg accgtctgcc ggaagaaaaa aagcgcggca
     2581 tgaccatcga tctcggctat gcctactggc cgcagccgga tggtcgcgtg cctggtttta
     2641 tcgacgttcc cggtcatgaa aaatttcttt ccaacatgct ggcgggcgtt ggtggtatcg
     2701 atcacgcgct gttggtggtg gcgtgcgatg acggtgtgat ggcacagacc cgtgagcatc
     2761 tggcgatttt gcagctgacc ggtaacccga tgctgacagt ggtgctgacc aaagccgatc
     2821 gcgtggacga agcgcgtgtt gatgaggttg aacgtcaggt aaaggaggtg ctgcgggaat
     2881 acggttttgc tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcga ggaattgatg
     2941 ccctgcgcga gcatctgctt cagttaccag aacgtgaaca cgccagccaa catagtttcc
     3001 gcctcgcgat cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc accggtacgg
     3061 cgttaagcgg ggaagtgaag gtaggcgatt cactatggct aactggtgta aataaaccga
     3121 tgcgtgtacg tgcgctgcat gcacaaaacc agccaacaga aaccgcccat gccgggcagc
     3181 gtatcgcgct taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt ggcgactggc
     3241 tgcttgccga tgcgccgcca gagccgttca cacgggtgat tgtcgagctt caaacccata
     3301 cgccgctgac ccagtggcag ccgctgcata ttcaccacgc cgccagccac gttacgggac
     3361 gcgtttcgct gctggaagat aaccttgccg agctggtgtt cgacacgccg ctgtggctgg
     3421 cggataacga tcgcttagtg ctgcgcgata tctcggcccg caacacgctg gcgggggcgc
     3481 gtgtggtgat gcttaacccg ccgcgtcgcg gtaaacgtaa gccggaatat ctgcaatggc
     3541 tggcgtctct tgcacgggcg cagagcgatg ccgatgcgtt atctgttcat ctggaacgcg
     3601 gcgcggttaa ccttgcggat ttcgcctggg cgcgccagct caacggcgaa gggatgcgcg
     3661 aattgctgca acagcctggt tatattcagg ctggttatag cttgttgaat gcgccggttg
     3721 ccgcccgctg gcagcggaaa attctcgaca cattagcgac ttatcatgag caacatcgcg
     3781 atgaacctgg tcctgggcgc gaacgtctgc gacgtatggc gttgccaatg gaagatgaag
     3841 cgctggtact gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc agtcatcacg
     3901 gttggctgca tctgccggat cacaaagcgg gcttcagcga agagcagcag gccatctggc
     3961 aaaaagcaga gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg gcaaaagaga
     4021 cgggtaccga cgagcaggca atgcgcctga cgctacgcca ggcggcgcag caaggaataa
     4081 ttaccgcgat cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa tttgccaata
     4141 tgatccgcga tctcgatcag gagtgtggtt caacctgcgc ggcggatttc cgcgatcgct
     4201 taggcgtagg ccgaaagctg gcaattcaga ttctggaata ttttgaccgc attggcttta
     4261 cgcgtcgtcg tggaaatgat catt
//
LOCUS       DAZFRJ010000279         4269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000279 DAZFRJ010000000
VERSION     DAZFRJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.281"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..515
                     /locus_tag="ACHKUN_004530"
     CDS             <1..515
                     /locus_tag="ACHKUN_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662646.1"
                     /translation="MIDPDPSVLKPDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVV
                     FSAKKRQWGSQEDLVCAQWIWGRIVSLYEQAASYDGEITRPKEPNWTAWANDVRTMRM
                     LDGRTHRQICEMFGRLQRDSFWVKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEP
                     DTEIPPGFRG"
     gene            522..1263
                     /locus_tag="ACHKUN_004531"
                     /pseudo
     CDS             522..1263
                     /locus_tag="ACHKUN_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1289..2050
                     /locus_tag="ACHKUN_004532"
     CDS             1289..2050
                     /locus_tag="ACHKUN_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6662647.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            2083..2364
                     /locus_tag="ACHKUN_004533"
     CDS             2083..2364
                     /locus_tag="ACHKUN_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6662648.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2361..2588
                     /locus_tag="ACHKUN_004534"
     CDS             2361..2588
                     /locus_tag="ACHKUN_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662649.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2581..2892
                     /locus_tag="ACHKUN_004535"
     CDS             2581..2892
                     /locus_tag="ACHKUN_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662650.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            3198..3776
                     /locus_tag="ACHKUN_004536"
     CDS             3198..3776
                     /locus_tag="ACHKUN_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFO6662651.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(3736..>4269)
                     /locus_tag="ACHKUN_004537"
     CDS             complement(3736..>4269)
                     /locus_tag="ACHKUN_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO6662652.1"
                     /translation="NNLAHLNEYRKVILSGGSFPTDLSDISVGLYSQPRIEWILWQSL
                     MNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDNDFWVFRGRVANRFGYE
                     QYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSGNYKFGSPEVWRRIGQ
                     NHHITKVVEQLATLYGL"
BASE COUNT         1187 a          883 c         1186 g         1013 t
ORIGIN      
        1 cgatgattga ccccgatccg tcagtattaa aacctgatcc gtcagataaa agatcttctt
       61 gtccggacgc ttcgcaaccg gacccgcaga cggctgaaca ggatttttta acccgacacc
      121 ctgacgcggt tgtgttcagt gcgaaaaaac gccagtgggg aagtcaggaa gatttggtgt
      181 gcgcacagtg gatctgggga cgaatcgtga gtctttacga gcaggcggcc agctatgatg
      241 gcgagatcac tagaccgaaa gaacccaact ggacagcatg ggccaatgac gttcgcacaa
      301 tgcggatgct ggatggcaga actcacagac aaatttgtga aatgtttggg cgtctccagc
      361 gggattcgtt ctgggtaaaa aacatcatga gtccggcaaa actccgggaa aaatgggatg
      421 aactggttat ccgcctgggg cgttcgcccg cgcagcgttg cgtgaatcac atttctgaac
      481 cggacactga aataccgccg ggattcaggg ggtgacgtgt catgaaaaac attgcggcag
      541 ttggggttct tgaacgtatt cgcagacttg caccacaggg gggcggttcc accgtaccgg
      601 acggtggagg agtggcggga atggcaactt gctgaaggac gaaaacgcag cgaggagatt
      661 aaccgcctga atcatcaggt gcgggttgaa aaaatcctga accgtgcggg catccagccg
      721 cttcacagga agtgctcatt cgggaactac cgggtgcaga acgacggtca gcgccatgct
      781 ctgagccagg cgaaatccat tgccgatgaa ttgatgaccg gatgtacaaa cttcgtgttc
      841 agcggtaaac ctggtaccgg taaaaatcac ctggcagcag cgattggcaa tcggctgatg
      901 gcgaagggga gaagcgtgat tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgac
      961 ggctacgaca acggccagtc cggggaaaaa tttttacagg agctttgtgg agttgacctt
     1021 ctggtccttg atgaaattgg catgcagcgg gatacgcgca acgagcaggt cacgctgaac
     1081 cagatagtcg accgcagaac ggcttcgatg cgtagtgtcg gaatgctgac gaacctgaat
     1141 cacgcagcga tgagcacact cctcggagat cgggtgatgg accgtatgac catgaatggt
     1201 ggtcgttggg tgaattttaa ctgggagagc tggcggtcaa acgttggacg tcagggtatg
     1261 tgagaatttt tgacgaggta aattttcgat ggaaactgta ttgcatgcac tgaaagcgat
     1321 gggaaaagcc aattctgttg aactggcggc gcggcttgat atcagccgtg aagaagttct
     1381 taacgaactg tgggaactca aaaaaaatgg cgttgttgat aaaacgggtc acacctggtt
     1441 tctggctgtc gaaggtgaag ccggggtaac cgaagggcag gcactacaac ctgaagcgcc
     1501 ggatgtggta accgaagagg tcgctccaaa agttaccgca gacatgatgg ttgagtttat
     1561 cggtcaggat ggtgctaaaa cgtgtgagga actggcgggt aagttcggcg tcagtactcg
     1621 caaggttgct tccacgctgg cggtggtaac cgcaacgggg cggctggcac gcgttaatca
     1681 gaacggtaaa tttcgttact gcatgtcggg gggtaattta ccagcagatc cgaaagccgc
     1741 gccggtaacg aaaaatgatg gtaaggcctt tcctcagcca gcaggtgctg cgttaccagt
     1801 ccgggaagcc gcaacacagg aagaaataaa aacggaaagt gtggcggtca cagtgcagtc
     1861 acagccgtcg ttcaccagaa agcatccgga tggtctgatt ttaccatcgc tgcatgtggc
     1921 taaccgcgag ctgcgccggg caaaaggtca ggttcagaag tgggagcgag tctgcgccgc
     1981 gctgcgggag ctgaacaagt gccgggatat tctccgggat attaccgcca ccagagaaca
     2041 gcagcggtga gtgggtggaa gacgtggtgc cgggcggaaa tcatgatact ccggcagtgt
     2101 gcgggaacga tgaaggtaaa aagcgttggc gcacttatcg gacgaactga agcggcagtg
     2161 agaacgaagg cacgggagct gggcatcagc atgatgttac gtggtgattt tcacccgtcg
     2221 gcaaaatatt ctcagcgtga tattgagctg gcgcggcaac tgcatcagag aggcatgcaa
     2281 agaagggaaa ttgccagaaa attaggcatg ccgctgcgca tagtgaataa ctacgtttat
     2341 ttcgacagga gggtgtctgc gtgaaaatcc tgtatcagga ttacggcccg gtggggcagg
     2401 tggttatcag cagtactgta atggagtttc ggaagcataa ccgtgtggtg gatgctgtgc
     2461 tgttaacctg tccggggata tcggcgagtc gtgcaggtgt gtttattatg aagacgaaat
     2521 tatatggcag taaggcgtgg ataaagaagg cgtatcgtgt agcgttgcag gaggttaaca
     2581 gtgagtgaaa ttaaagaaat gccggtagtt cgtgacggat atggctactg gacacatcct
     2641 gaatatgaaa aattctgtga tggtcgggaa tatatttcaa cggaagagtt taacgcctgg
     2701 atggaggaaa ataatcttca atacgtcctc tgcttcagag atgaaggatg tgctgacctt
     2761 gatgcgtgtg atgctgatat ttctgcatgg gaaccggaac gaccagaggg caatggatgg
     2821 tttattggtt caatacatga caccgaagat ggcccggttt gtgtatggct gagaaataag
     2881 gccgaagcat aaaggctata aaccgactaa caactaaata ctgaagattt aaatcagaaa
     2941 cgatttttat taaaccctta accggaggga ttctgcaccc tcagaacatc aggaggccgt
     3001 ctgaaagggc ggaacagata atgcttacgt tgaaacattt tatcgacata ccaacatggt
     3061 tagccgtcat tgcttttgtt aaaatacaca tccacttttc tgtgcaatgt ttaaccactg
     3121 gtcatatcaa atggcattca tgcgaaccat gatatagaat catggcttga gagagtcgat
     3181 gaaagcgcaa ctatggtatg agagacattg atgtaagaaa ggctgtgcat gccaagattc
     3241 tgagagatca tcataaagat cctgacaccc taatcattga tgagtttacg atgaatctag
     3301 gggctagcag agctgatata gcagtgatca atgggcttat acatggttat gagttgaaga
     3361 gcaagagtga taacttgctc agattaccag cgcaggtgca acattactca tcagtgatgg
     3421 ataaagtaac tttggttgtc tctgattgcc atctttatga tgctttaagc atagttccat
     3481 catggtgggg gataaagcaa gttacgcaag gtgcacggca aggtatccat ttaaaaacaa
     3541 ttcgaactag caagttgaat ccacaagtgg acaaactttc cttaacaatg cttctttgga
     3601 aagatgaatt gctttcccta ttaagtgatg taggggagct acagaatttg aaaaataaac
     3661 ctaaacgcgt cttatggtca aaactcgcca atagtatgga tgttggcgag cttcgtgaag
     3721 ctgttcgagt taaacttaaa gcccgtaaag agtggcgagt tgctcaacaa ccttagttat
     3781 gtgatggttt tgcccaatcc tacgccatac ctctgggcta ccaaatttat agttaccaga
     3841 gggattggct ttgtaggctt gatactcgtt tgcataatat tctatgtctc tatctcccgc
     3901 acagaatgta ggccctgaat attctcgatg agcaagaata tcctcactat gtttaccata
     3961 ttgttcataa ccaaagcgat tagctactct tcctcgaaat acccaaaagt cattatctcc
     4021 agagtatctg acgctggcag atacgctagg gaatcgcgtc gaaagcctat taaagtcggg
     4081 gtgctgtact ccataatcac tataaatcac atttctggca agttcttttc tattcattaa
     4141 actctgccat aaaatccact cgattcgagg ttgagaatat agaccaacag aaatatcact
     4201 gagatctgta ggaaatgaac ccccagaaag aatcactttt ctgtattcat tgaggtgcgc
     4261 cagattgtt
//
LOCUS       DAZFRJ010000280         4196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000280 DAZFRJ010000000
VERSION     DAZFRJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.282"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..944
                     /locus_tag="ACHKUN_004538"
     CDS             <1..944
                     /locus_tag="ACHKUN_004538"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFO6662653.1"
                     /translation="KKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRKRTVICLDI
                     TNFYPSISKKDVCSIFKNLMRFSPEVSLCLTELVTLNNKVPQGGCCSSYIANLLFFNS
                     EYNLYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYRLSINESKT
                     TIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERHTKEYHDLW
                     NRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNKFTPPLTDE
                     QIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
     gene            949..1152
                     /locus_tag="ACHKUN_004539"
     CDS             949..1152
                     /locus_tag="ACHKUN_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="HFO6662654.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICK
                     GILVRFKLEKTDKGFKAVAIQKV"
     gene            complement(1989..2183)
                     /locus_tag="ACHKUN_004540"
     CDS             complement(1989..2183)
                     /locus_tag="ACHKUN_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO6662655.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            2468..2791
                     /gene="mobA"
                     /locus_tag="ACHKUN_004541"
     CDS             2468..2791
                     /gene="mobA"
                     /locus_tag="ACHKUN_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO6662656.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            3041..3427
                     /locus_tag="ACHKUN_004542"
     CDS             3041..3427
                     /locus_tag="ACHKUN_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662657.1"
                     /translation="MNISIKNNDLKTIARFDGKTLRDAFSRKIATFDGKILRDASLKK
                     IATFDGKVLRDASLKKVATFDGKVLRDASLKKVATFDGKVLRDASLKKVATFDGQNLR
                     DASSKIILSADAEVGIVVMAFVAELI"
     gene            complement(3976..>4196)
                     /locus_tag="ACHKUN_004543"
     CDS             complement(3976..>4196)
                     /locus_tag="ACHKUN_004543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662658.1"
                     /translation="IPFKDLLGSKICLSFLFFPVSTLNCLYDIDTDLTIQSNLFLSMP
                     NIADKESKDATGVPLNLGLFMMFELKVD"
BASE COUNT         1157 a          785 c         1000 g         1254 t
ORIGIN      
        1 aaaaaaaaat aaataaagaa atttttgaga aaattgatta ccctcattat ttgcatgggg
       61 cgttaagtgg tcgcgattat atatctaatg ccgctgttca tactaggaaa agaacagtta
      121 tatgtcttga tatcaccaat ttttatccca gcattagtaa aaaagatgtt tgttctattt
      181 ttaaaaattt aatgcgtttt tctcctgagg tttctttgtg cttaacggaa cttgtgacgt
      241 tgaataataa ggttcctcag ggtgggtgct gttctagtta tattgcaaat cttttatttt
      301 ttaatagtga gtataatctc tataatagat tgaagtctat ggggctttca tattcaaggc
      361 ttttagatga tattactata tcctctgata aggatctttc atcagaggaa aaaacaaaag
      421 taataaaact cgttcacgga atggtgaatc agtacagact ttcaataaat gaaagtaaga
      481 caacgataga gcactcaaaa gattcgtctt ctaaacttag tgtcactggg ttgtgggtaa
      541 agcatggcgt tcctaagtta acaaaagaaa atagacgtta tatcaggtac ttagtttata
      601 tatgtaaaaa acaaggtgct tatgaacgtc atacgaaaga gtaccatgat ttatggaata
      661 gatgctctgg caaggttgct caaatgtcga ggcttgggca tgtccaggcg gttgaattga
      721 gggcgattct ttctgagata atgccagttt atgatgatta caagatatct aaattaaagt
      781 tgatggctaa gcattatcta aataaattta cgccgccttt aactgatgag cagataagga
      841 aaatagatag gatgctctat gattttgata tagtagggcg gactaataag aatcttgcaa
      901 aactatatag acgtaagctt gtggcattac ttccagatag gtgattatat ggaaaatgga
      961 tttgttaatt tttatgacca tgttaaaggg tatggcttta taaggcgtga gcgtggacgt
     1021 gatgttttct ttcggtatga tgattttttg tttttagggc atgatgtcga tatatgtaaa
     1081 ggtattttag ttaggtttaa attagaaaaa acagataaag ggtttaaagc tgtagcgatt
     1141 caaaaggtat aacatcgtgg atgtgtcagc ttgtttttat gatatgagaa cttgagatcc
     1201 tttctatctc gtcgtaatct cttgctgtgt aaacgaaaaa accgccttgc agggcggttt
     1261 ttcgaaggtt ctcagagcta ccaactcttt gagccgaggt aactggcttg gaggagcgca
     1321 gtcaccaaaa cttgttcttt cagtttagcc ttaacaggcg catgacttca aggctaactc
     1381 ctctaattcg gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt ctcggtttgg
     1441 attcaagtcg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca
     1501 tacagtccag cttggagcga acgacctaca ccgggccgag ataccaacag cgtgagctat
     1561 gagaaagcgc cacgtttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1681 ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt gtgatgctcg tcaggggggc
     1741 ggagcctatg gaaaaacgcc tcctgctcgc ccttcttctg ggcctttgtc ttttgctcac
     1801 atgttctttc cggctttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1861 gctgacaccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     1921 gaagagcgcc ttatgtgaca ttttctcctt acgctcctct atgccgttct gcatcctgtc
     1981 ctgatgcgtt atatcccggt gcgattttcc gcttcaaagc gtgtctgtat gctgttctga
     2041 agttcttccg cgagttcgtg cagcttctca cacatggctg cctgttcgtc agcatcaagt
     2101 gcgtccagtt tttcgagcag cgtcaggctc tggcttttta tgaatcctgc catgttgagt
     2161 acggtttgct gctgcttgtt catctttctg ttttctccgt tctttctgtc atctgctttg
     2221 tgtgattata tcgcgtacca cttttcgact gttttaccgt cggtattctg ctgtctgact
     2281 ctttgacggg catttctgtc agacaacact gtcactgcca aaaaactgcc gtgcctttgt
     2341 cggtaattcg agcttgctga caggacagga tgtgtaattg ttataccgcg catacatgca
     2401 cgctattaca attaccctgg tcagggcttt gccccgacac cccatgtcag atacggagcc
     2461 atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg cgggctatca gtcgccctgt
     2521 tcgtctgacg gcagaagaag accaggaaat cagaaaaagg gctgctgaat gcggcaagac
     2581 cgtttccggt tttttacggg cggcagctct cggtaagaaa gttaactcac tgactgatga
     2641 tcgggtactg aaagaagtta tgagactggg ggcgttacag aaaaaactct ttatcgacgg
     2701 caagcgtgtc ggggacaggg agtatgcgga ggtactgatc gctattacgg agtatcaccg
     2761 tgccctgtta tccaggctta tggcagatta gcttcccggg gagaaaactg tcgaaaacag
     2821 acggtatgaa caccgtaagc tcccaaagtg atcaccattc gctttcatgc atagctatgc
     2881 agtgagctga aaacgatcct gacgcatcct tcctgttttt tcggggctaa acctctcttt
     2941 ttgcggtatt tgcgtcagaa ttgcgttcag cgcgtttcag cggtgcgtac aattaaggga
     3001 ttatggtaaa tacaattggt taaattacta ttggtgataa atgaatattt caataaaaaa
     3061 caatgactta aaaactattg ctcggtttga cggtaaaacc ttaagagatg ccttttcgag
     3121 aaaaatagcg acttttgatg gcaagatttt aagggatgct agcttaaaaa aaatagcgac
     3181 ttttgatggc aaggttttaa gggatgcctc tttaaaaaaa gtggcgactt ttgatggcaa
     3241 ggttttaagg gatgcctctt taaaaaaagt ggcgactttt gatggcaagg ttttaaggga
     3301 tgcctcttta aaaaaagtag caacctttga cggtcagaat ttaagagatg ctagttcaaa
     3361 aattatcctg agtgccgatg ctgaggttgg gattgttgtt atggcctttg tagctgagtt
     3421 gatttgaatc tcatgctgca taccttttag cctatcgctg gcaggataag gcgctttctt
     3481 gttggtgaca gaaagataaa aaactttata ataatcagcg aagcgggcga accacgcctg
     3541 gctctcgctc tttaacaaat ggttacgtgt atgttgcgca aggaaaccga tgccttgttt
     3601 ttaaggcgat ctcgcatgag atcatgaccg gcctggccgc taattaatgg tgagaacgcc
     3661 cctgagcgtg tatcagcact aaaagaatag cgacgattgt ctggcaatgt gagttaaaag
     3721 tcccaaagag ccaatagagt ttttccttgc tttcctactg gtctggcaaa atttcataac
     3781 caataggagt tgcatcatgt ttattatcaa catcaacatt aacgtaacca agagctcttc
     3841 atcttgttct ggagacggtc agatgaagaa aaaaagagag gttggctctc tggaggcact
     3901 gaaaaaaaca cagtttttgt tttctatgtg cttgattatg tttaaagtaa tagattacgt
     3961 atcgaaactt tttaactaat cgacttttaa ctcaaacatc atgaataaac ccaggtttaa
     4021 tggaactcct gttgcatctt tggattcttt atctgcgatg ttaggcatag aaagaaaaag
     4081 attggactgg atagtcaagt ctgtatcgat gtcgtacaag cagtttaagg ttgaaacagg
     4141 gaagaataaa aaagaaaggc aaatttttga acccaagaga tctttgaaag gaattc
//
LOCUS       DAZFRJ010000281         4094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000281 DAZFRJ010000000
VERSION     DAZFRJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.283"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..645
                     /gene="ydeO"
                     /locus_tag="ACHKUN_004544"
     CDS             <1..645
                     /gene="ydeO"
                     /locus_tag="ACHKUN_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO6662659.1"
                     /translation="HFEPYERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASN
                     VPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRN
                     IVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGS
                     VNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            1047..2729
                     /gene="ydeN"
                     /locus_tag="ACHKUN_004545"
     CDS             1047..2729
                     /gene="ydeN"
                     /locus_tag="ACHKUN_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6662660.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2781..3938
                     /gene="ydeM"
                     /locus_tag="ACHKUN_004546"
     CDS             2781..3938
                     /gene="ydeM"
                     /locus_tag="ACHKUN_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6662661.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3904..4026)
                     /locus_tag="ACHKUN_004547"
     CDS             complement(3904..4026)
                     /locus_tag="ACHKUN_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662662.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
BASE COUNT         1250 a          860 c          812 g         1172 t
ORIGIN      
        1 cattttgaac cttatgagcg gcattttaaa gagatcggat ttactgaaaa taccattaaa
       61 aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta
      121 cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa
      181 gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc
      241 gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc cggaaaagtg
      301 agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc
      361 ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca
      421 cagattctat tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca
      481 gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat ttatgcgttc
      541 cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaagtcac
      601 acgggtatga atacgggcaa cacgatgagt gctttagcta tttgattatt tgctaacgag
      661 tagtcaacca cgcacgctac gtaagaatta tatggggcag ccattccctg ccccgcgttg
      721 tttttaggcg atatatttat tgaaataaat aagtgacatc catcacatat ttatgcactt
      781 gcataacctg ttgcatgatt atttatgatc tcaattcggc attttgtcag taaaatgcaa
      841 taatttatta aatatcaata aattagttgt ttatcggcga gaaattactt aatagaacag
      901 aaagtaatgt caacgcttta tggactgtta tttccctttt tttagctaaa tctgctatct
      961 ctttatgtga ctaacttcac ttacatccac ttatttctct tcgtaaaatt acttcggaat
     1021 taagtacaat aagaagagga acatttatga agtctgcatt aaagaaaagt gtcgtaagta
     1081 cctcgatatc tttgatactg gcatctggta tggctgcatt tgctgctcat gcggcagatg
     1141 atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc agactttacg ccgactgaat
     1201 acagtaccaa aggaaagcca aatattatcg tactaaccat ggatgatctt ggttatggac
     1261 aacttccttt tgataaggga tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg
     1321 atacctacaa aatagggata gataaagcca ttgaagctgc acaaaaatca acgccgacgc
     1381 tcctttcatt aatggatgaa ggcgtacggt ttactaacgg ctatgtggca cacggtgttt
     1441 ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt
     1501 ccaataccga tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc
     1561 agaatcatgg ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg
     1621 tgccggtacc ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg
     1681 cggaagaatg gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag
     1741 gaacggcata ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag
     1801 gttatatcag cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac
     1861 ttgaccagcc ttttatgctt tacctggctt ataatgctcc gcacctgcca aatgataatc
     1921 ctgcaccgga tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact
     1981 acgcttccgt ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa
     2041 acggacagta tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg
     2101 gtcctctgcc gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta
     2161 ctcacacccc aatgtttatg tggtggaaag gaaaacttca acccggtaat tatgacaagc
     2221 tgatttccgc aatggatttc tacccgacag ctcttgatgc agccgatatc agcattccaa
     2281 aagaccttaa gctggatggc gtttccttgc tgccctggtt gcaagataag aaacaaggcg
     2341 agccacataa aaatctgacc tggataacct cttattctca ctggtttgac gaggaaaata
     2401 ttccattctg ggataattac cacaaatttg ttcgccatca gtcagacgat tacccgcata
     2461 accccaacac tgaggactta agccaattct cttatacggt gagaaataac gattattcgc
     2521 ttgtctatac agtagaaaac aatcagttag gtctctacaa actgacggat ctacagcaaa
     2581 aagataacct tgccgccgcc aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag
     2641 agtttatcga cagcagccag ccaccgctta gcgaagtaaa tcaggagaag tttaacaata
     2701 tcaagaaagc actaagcgaa gcgaaataac taaaccttca tgcggcggaa ttttccgccg
     2761 ccttattgag cgagatagcg atgcacgtta cagccaagcc ctccagtttt caatgtaatc
     2821 tcaaatgtga ttactgtttt taccttgaaa aagagtcgca gtttactcat gaaaaatgga
     2881 tggatgacag cactctgaaa gagttcatca aacaatatat cgcagcgtct ggcaatcagg
     2941 tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt ttccgtaaag
     3001 ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga
     3061 atggcatttt attgaataat gaatggtgtg cttttctcaa agaacatgag tttctggttg
     3121 gtatctcgat cgatggcccc caggagctac atgaccgtta cagacgcagt aattcaggta
     3181 acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat caagttgagt
     3241 ttaatacgtt aaccgtcatt aataacgtta atgtccatta ccctcttgag gtttatcatt
     3301 ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctcgaa accgggacgc
     3361 cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat ttttctgtgc
     3421 ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg
     3481 tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag ccgttttttg gggaatgggc
     3541 ataccagttg tattttccag gagtcctgca aggataatct ggttgttgaa agtaatggag
     3601 acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac attaataaat
     3661 ctgaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg atttcagcga
     3721 aatgtcagca atgtgcatat aaacctatct gcaatggcgg ttgtcctaag catcgtatta
     3781 ctaaagtaaa caatgagact gtttcttatt tttgcgaagg ttataaaaac cttttttcaa
     3841 ccatggtacc ttatatgaac gccatggtgg agttagctaa gaacagagta ccgctttatc
     3901 acattatgga tgttgcaaaa caaatggaga ataattaata acacgccctt ttagctccat
     3961 tcttagcctc cttcttatga ccttaacaag gcacccgaca taaattcctg tcgggtgttt
     4021 cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag
     4081 caataccctt gatt
//
LOCUS       DAZFRJ010000282         4082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000282 DAZFRJ010000000
VERSION     DAZFRJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.284"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..67)
                     /locus_tag="ACHKUN_004548"
     CDS             complement(<1..67)
                     /locus_tag="ACHKUN_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662663.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHKUN_004549"
     CDS             complement(64..639)
                     /locus_tag="ACHKUN_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662664.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHKUN_004550"
     CDS             complement(654..1007)
                     /locus_tag="ACHKUN_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6662665.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKUN_004551"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKUN_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6662666.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..2310)
                     /locus_tag="ACHKUN_004552"
     CDS             complement(1426..2310)
                     /locus_tag="ACHKUN_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6662667.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVASSRYPKHWLTVGDPAREFTMTQS
                     APLMVLPDPDEFVVVQVK"
     gene            complement(2368..2715)
                     /locus_tag="ACHKUN_004553"
     CDS             complement(2368..2715)
                     /locus_tag="ACHKUN_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6662668.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(2752..>4082)
                     /locus_tag="ACHKUN_004554"
     CDS             complement(2752..>4082)
                     /locus_tag="ACHKUN_004554"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6662669.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          862 a         1289 c          982 g          949 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcactgc
     1621 cgtttcgagt tgtgaatttg agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga cggtaccgtc cataatggca atattcacaa gaccgctggc
     1741 ctggtcgcag tagaggtcaa gatcatgtgt aggatcgaac gtgtcacgat cctgttttga
     1801 ccactcctta ccactaccct gagtgatgtt attcttcgtc gacctgccaa aatcgacctc
     1861 aattttctcg aactggtctc cttccatcgt gtatttgcca tacagcacgg cattcaccgc
     1921 ctgcatttct tccacccgga caatggcgtg ctcttcctgt ttgaggttat cagtgatgat
     1981 acgcagacga cggtaggccg ggtcgttcag ctgagccgga tcttcaccgg gaagacgctc
     2041 aaccgcctgc tggtaattaa attcgtgttt cggcttgacg tagcccggac gtaacacgcg
     2101 ggtttcacca ccacgatgac gcagcacttt tccttcaaca accggggaaa cataggccgc
     2161 caccggcgtt tttccggtaa ttttgtccag catcacctct tcggtatgga aattcaccgt
     2221 acggcggaaa aacagctcca gaaacagcgc acgaaatttc actttttgtt cggtataacc
     2281 gagtaactgg cgggtcgtaa acaatcccat aaatcagttc ctttcattca gaaatcagtc
     2341 aggccaccgc ggtggcctga taacgtgtta cggcagcgcc gcgtgactca gggcactgcc
     2401 ggcaaaggcg ttggcctttt tgtgttcatc cacactttca ggccagcgga ttgcctccgt
     2461 cgcaaaggtc cccgacttgt aataggtcag cgccgtctct gtgccttcaa gcggcagtac
     2521 cagtatgcca accgcactac cggctttctg tccgtcccag accaccagtt tcccggtggc
     2581 ctcatccagc atcaggggcg tcagtgccgg tgttgccgag gaaatcccgc tgctgcctgt
     2641 ggcggtatga gccggatcat taccggcaaa aatacgtact tccacacgct gttcagtgat
     2701 agttttcgtc accatattgt aaaaacctcc tgttgatggt cagcactggc ttcatggcat
     2761 ggccatgagc attttcacgt ccgcatcacc gtctgctgac gtctgtggca cgccaccctg
     2821 taccgctgcc ggtgaatggt tcgccatgat gcgttcaaac atggcggttg tggatgcaga
     2881 gaccggttct gccttacctg atcccgcagc cagcacagcc cgggcgctct ccacagtcat
     2941 tcccgggcag gcagccagct gttcagcctg cgcctcagcc ccttttgcct catccagtgc
     3001 catgatctga tcacggagtg agggtccggc atccgcctgc ggtgaagcag ccaggatcgg
     3061 gcgggctttt tccaccgtca tctccggcat cgccgccagc gttgccgcca gttgttcacg
     3121 acctttcgct tcttcacacg ccataatgcg atcggcttca ctctgcgcgg atgccaccgg
     3181 ctgctgtggt gccgccgcgg ccagaatcgc ccgggcctgt tcaacgctca tgccctgttg
     3241 tcctgccagc atcgtggcaa gctgttcacg tcctttcgct tcctggcatg tcaggatccc
     3301 catcactcgc tggttctcct gcgcggcggc ttccgttgca gttaattgcg gcatagtgcc
     3361 tcctctgaca ttactgttca gcgccgtggc catcacactg atggcatccg acgcattgat
     3421 taattcatcc gccagcccgg cctcaatgcc ggactgacct tcaaaaacgg cggcctctgt
     3481 tcccgtgaca gcatcaacag acagaccggt atacatcgct actttttcag caaacatccg
     3541 gcgcgccgca tcaatgcgct gttgcatgtc ctggcgaacc tctgccggca acgcttcaaa
     3601 ctgattgcca tccaccttgt gcgcccctga gtaaatcagc gtgatatcaa caccggcctg
     3661 cgccagatga ccggcatagc tgacatggct catcatcacg ccaatggagc cgatacggga
     3721 tgtctgggta accagccgtc gggagcaggc cgacgccagc agcatggctg cagagcaggc
     3781 cgtgtcattg cacagtgccc agaccggctt ctgctgacgg aggcggtaaa tcatgtcagc
     3841 gcagtcaaac gcgccggcgg cctgcccgcc cggactgtca atgtccagca gtacgccccg
     3901 cacctggcta tccgccattg cctgctgaag acaggcgaca atgccgtcat agcctgtcat
     3961 tccggaaaat ggccgcatac cccccagccg gtgcaccagc gtgccggtca ccggcagtac
     4021 agcaataccg ttcaccaccc ggtaaacacg ggccggtcgt ttacctccgg ccatgtactc
     4081 gt
//
LOCUS       DAZFRJ010000283         4059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000283 DAZFRJ010000000
VERSION     DAZFRJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.285"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..1029)
                     /locus_tag="ACHKUN_004555"
     CDS             complement(34..1029)
                     /locus_tag="ACHKUN_004555"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO6662670.1"
                     /translation="MSYSEIVKKYENISKSTNYSINILDKLEDDLQKIFSEKNLEFTM
                     VTTGSFGRLEASEESDIDLFIFCKKESEKHYILYKKEEIEACIKKYIHKKAGDTGTFG
                     VDAVDVFDNILKNIGGGDDTNKSLTRRMLFLLEGKAIYNKNIFNSYKKKLITKYLNSS
                     SEGKLDKYLLNDVIRYYRTITTDFQHKVDADGKSWGIRNIKLRYSRKLLYFAGVMAIA
                     HASETQQDITKRTQEISNSLDIPPLERIYNISHGKAKPELIEDIFCQYEFFLSCISDP
                     EKRASLNDIKDKSDRLNSPLYNQLSEKSTEFTDNLYKLLNNAFEKNNQIHTSLIF"
     gene            complement(1058..2086)
                     /locus_tag="ACHKUN_004556"
     CDS             complement(1058..2086)
                     /locus_tag="ACHKUN_004556"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO6662671.1"
                     /translation="MGDFFKRNDIDEEIEKESIGYWIQDLQKSSKLLMIIKTAAFNRL
                     YGISFLGAIDYSDQNELTKKERNRAIHSLYVAGIANYVATKRNYNSDLKEHLVAAALL
                     HDIGHMPLSHSAEPFIKEKFGYGHHELGNNLISGTCWVDSGLSKALNKNFDVSFIKGL
                     LNFELKTDGHDIFSNKINADTIDGIVRCIEYKGKNKANHLNRISIAKAAFLDNIDMTE
                     NKRLQILDNFWNTKHFVYQNFINTKHSVISDKISQIFFMENKKIAYKNLLSDERRWRE
                     QYKLLFKWLSNLKSNSIPHCLKNYEMIYTTRNYKVLKTEKDIKKRYTNNKNKAKISED
                     SLLKYREE"
     gene            complement(3076..3270)
                     /locus_tag="ACHKUN_004557"
     CDS             complement(3076..3270)
                     /locus_tag="ACHKUN_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO6662672.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQVRFESESETGT"
     gene            complement(3879..>4059)
                     /locus_tag="ACHKUN_004558"
     CDS             complement(3879..>4059)
                     /locus_tag="ACHKUN_004558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662673.1"
                     /translation="HLNPQHKPIYHNISLYAPQKRAERDSDAGTAKRDVLPSENRKNA
                     SGSFSAHCIAMHENE"
BASE COUNT          981 a          834 c          833 g         1411 t
ORIGIN      
        1 aaaaaaaaag gccgcttgaa agcggccttt aagttaaaaa attaatgagg tatgtatttg
       61 attgttcttt tcaaacgcat tattaagcaa tttatataaa ttatctgtaa attctgtgct
      121 tttttcgctc agttgattgt aaagagggga gtttaatcta tctgatttat cttttatgtc
      181 gtttaaacta gctcttttct ctgggtcact gatgcagctt aagaagaact catattgaca
      241 gaagatgtct tctatcagtt caggttttgc tttgccgtgg ctgatattat atattctctc
      301 taaaggtggt atatctaaac tattagatat ttcttgtgtt cgttttgtta tgtcttgttg
      361 tgtttcactg gcatgggcga tagccattac gcctgcaaaa taaagcagtt tacgggaata
      421 gcgtagtttt atatttcgga taccccaaga cttgccatcg gcatcaactt tatgctgaaa
      481 gtcagtagtg atagtcctgt aatatcttat aacatcgtta agcaaatatt tgtcgagctt
      541 tccttcgctt gaggagttca aatatttggt aattaatttt ttcttgtaac tattgaaaat
      601 gtttttgtta tatattgctt tcccttctag tagaaacaac atccttctgg ttagagattt
      661 gtttgtatca tccccaccac caatgttttt taaaatatta tcaaatacat ctaccgcatc
      721 aacgccaaac gtacctgtat caccggcttt tttatgaatg tattttttta tgcaagcctc
      781 tatttcttct tttttatata gaatgtagtg tttttccgat tcctttttac agaaaataaa
      841 gagatctata tcagattctt ctgacgcttc tagtctaccg aaagatcccg tggtgaccat
      901 ggtgaactct aaattttttt ctgaaaatat cttttgtaga tcatcctcta gtttgtcaag
      961 aatgttaatg ctataatttg ttgattttga aatgttttcg tattttttga ctatttcact
     1021 atagctcatt ttttttatcc ctacactctt actggtatca ttcttctctg tattttaaaa
     1081 gactgtcttc acttatcttt gctttatttt tgttgtttgt atatcttttt ttaatgtctt
     1141 tctctgtctt taacacttta taatttcttg ttgtgtatat catctcgtaa ttttttaggc
     1201 aatgaggtat tgagtttgat tttaggtttg ataaccattt aaacagtaat ttatattgct
     1261 ctctccagcg tctttcgtct gagagtaagt ttttgtatgc tatcttttta ttttccatga
     1321 aaaaaatctg acttatcttg tctgatatga cgctatgttt cgtattgata aaattttgat
     1381 atacaaaatg ctttgtattc caaaaattat ctaatatctg cagcctttta ttctctgtca
     1441 tatctatgtt atctaagaat gcagcttttg caatagaaat tctatttaaa tggtttgctt
     1501 tattctttcc tttgtactct atgcacctca ctatgccatc gatagtatcg gcatttattt
     1561 tatttgagaa gatgtcgtgt ccatcggttt ttagttcgaa gtttaacagt ccttttataa
     1621 agctaacgtc aaagtttttg tttagagcct tgcttaaacc gctgtctacc cagcatgtgc
     1681 cagatattaa attatttccg agctcatgat ggccataacc aaatttttct tttataaacg
     1741 gctcagcgct atgcgataac ggcatatgtc ctatgtcatg cagtaaagct gcggctacta
     1801 aatgctcttt gagatccgaa ttgtaattcc ttttagttgc tacatagttt gctatccctg
     1861 cgacatataa agagtgaatt gctctgtttc tttctttttt tgtgagttcg ttttgatcgc
     1921 tataatcgat agcacctaag aatgatattc cgtataagcg attaaatgcg gcagttttta
     1981 ttatcattaa taatttactg gacttttgta agtcttgaat ccagtatcca atgctttctt
     2041 tttcaatctc ctcatcaatg tcatttcttt tgaagaaatc acccataata tctgctctca
     2101 aatcattcac gttaaattga ctctaacact taatatgaca acttgctcac acaatccagt
     2161 tgacgattcg tgtcatgtca agatactgtt tacctcgttg aaaatgaaaa aaccgccttg
     2221 gcgggcggtt tttcaaaggt tctctagagt tggcgctctt ttgaaccgag gtaactggct
     2281 gatagaggag cgcagtcacc aaaacttgtt ctttcagttt agccttaacc ggcgcataac
     2341 atcaagacta actcctctaa atcagttacc aatggctgct gccaatggtg cttttgcatg
     2401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     2521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcggc gcggtctttt gctctggctt
     2761 tgcctttttg ctcacatgtt ctttccggcc ttatcccctg attctgtgga taactgtgtt
     2821 accgtttttg tgtgagtcag taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca
     2881 gtgagcgagg aagcggaaaa gcgcctgaat gtgcattttc tccttacgca tctgtgcggc
     2941 atttcacacc cggcgtgggg cacttttcat acaatctgca ctgatgccgc atggttacgc
     3001 cagtatacac tccgctatcg ctacgtgact gggtcatgtc tgcgccccga cacccgccag
     3061 ggcgcgtcag cttcgttatg ttcctgtttc actttcgctc tcaaagcgaa cctggatgct
     3121 gttctggagt tcttccgcga gttcgtgcag tcgttcgcac atggccgcct gttcgtcggc
     3181 atccagagca tccagttttt caagcagtgt caggctctgg cttttgataa atcgcgccat
     3241 attcagcgca gtttgttgct gtttgttcac cttccgttgt tctccgtcag taccgggtgt
     3301 cgggtggacc ctgaccaggt ggtaattgta agacgttgcg ggcgacggta ttacaattgc
     3361 acatcctgtc ctgtttcttt aggacgtatc atggcacagc atatcaactg ataaataatc
     3421 cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg cgtcgagtgt ttcttaatca
     3481 ataattccgt tgatactccg gaaaaacgcg agtctgttct catgcctgaa atgcccacac
     3541 cccacgcaaa aacaagtttt tgctgatttt cctttataaa tagagagtta tgacaaatta
     3601 gtttctcttg ctctctttgc gatatttaaa aaagcggtgt cggcgcggtg ttgtagctgc
     3661 actgacaccg ctttgtaggg gtggtactga ctatttttat aaaaaacatc attttatatc
     3721 aggggtgctg ctggcggcgc ggtgtgtttt tttacaggac gcccctgggg gcgctgctag
     3781 gggtgtctgt tcagatatgt agcaatgagt cagggattca cgaactgaaa atgtccgaaa
     3841 atgtgtctca tgggcaccct atgcgcccaa accgttcgtc attcattttc atgcatagct
     3901 atgcagtggg ctgaaaacga tcctgacgca tttttccggt tttcactggg taaaacatct
     3961 ctttttgcag tgcctgcgtc agaatcgcgt tcagcgcgtt tctgcggtgc gtacaaactg
     4021 atgttatggt aaattggctt gtgttgagga tttaaatgc
//
LOCUS       DAZFRJ010000284         4056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000284 DAZFRJ010000000
VERSION     DAZFRJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.286"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1816
                     /locus_tag="ACHKUN_004559"
     CDS             <1..1816
                     /locus_tag="ACHKUN_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6662674.1"
                     /translation="YYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVG
                     YSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKK
                     HRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLG
                     DKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKE
                     TPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSG
                     EEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGE
                     RPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIM
                     GRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRVI
                     PIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHF
                     PNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISIS
                     RWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1813..2019
                     /locus_tag="ACHKUN_004560"
     CDS             1813..2019
                     /locus_tag="ACHKUN_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO6662675.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2016..3617
                     /locus_tag="ACHKUN_004561"
     CDS             2016..3617
                     /locus_tag="ACHKUN_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6662676.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3598..>4056
                     /locus_tag="ACHKUN_004562"
     CDS             3598..>4056
                     /locus_tag="ACHKUN_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6662677.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDI"
BASE COUNT          924 a         1051 c         1253 g          828 t
ORIGIN      
        1 ttactatctc ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca
       61 gcgggccatc atgaatgcga tgggcagcga ctacatccgc gaggtgaatg tggtgaagtc
      121 tgcccgtgtt ggttattcca aaatgctgtt gggtgtttat gcctacttca tagagcataa
      181 gcagcgcaac acccttatct ggttgccgac ggatggcgat gccgagaact ttatgaaaac
      241 ccacgtcgag ccgaccatcc gtgatattcc atcgctgctg gcgctggccc cgtggtatgg
      301 caaaaagcac cgggataaca cgctcaccat gaagcgtttc accaatgggc gtggtttctg
      361 gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag tcagtggatg tggcgggtta
      421 tgatgaactt gctgcctttg atgatgatat tgaacaggaa ggctctccga cgttcctggg
      481 tgacaagcgt attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt
      541 gagaggcacc tgtcagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca
      601 tgttgcctgc ccgcactgcg gggaggagca gtaccttaaa tttggcgata aagagacgcc
      661 gtttggcctc aaatggacgc cggatgatcc ctccagcgtg ttttatctct gcgaacataa
      721 tgcctgcgtc atccgccagc aggagctgga cttcactgat gcccgttata tctgcgaaaa
      781 gaccgggatc tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga
      841 gccgccggac agtgtgacct ttcacatctg gacggcgtac agcccgttca ccacctgggt
      901 gcagattgtc aaagactgga tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt
      961 gaacaccacg ctcggtgaga cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt
     1021 gatggcagag cggaaagagc attattcagc gcccgttcct gaccgtgtgg cttacctgac
     1081 cgccggtatc gactcccagc tggaccgcta cgaaatgcgc gtatggggat gggggccggg
     1141 tgaggaaagc tggctgattg accggcagat tattatgggc cgccacgacg acgagcagac
     1201 gctgctgcgt gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat
     1261 gtcggtatcc cgtatctgct gggatattgg cgggattgac ccgaccattg tgtatgaacg
     1321 ctcgaaaaag catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggaaa
     1381 gccggtggcc agcatgccac gtaagcgaaa caaaaacggg gtttacctta ccgaaattgg
     1441 tacggatacc gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga
     1501 accgcttccc ggtgccgttc acttcccgaa taacccggat atttttgatc tgaccgaagc
     1561 gcagcagctg acagctgaag agcaggtcga aaaatgggtg gatggcagga aaaaaatact
     1621 gtgggacagc aaaaaacgac gcaatgaggc tctcgactgc ttcgtttatg cgctggcggc
     1681 gctgcgcatc agtatttccc gctggcagct ggatctcagt gcgctgctgg cgagcctgca
     1741 ggaagaggat ggtgcagcaa ccaacaagaa aacactggca gattacgccc gtgccttatc
     1801 cggagaggat gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc actgcatgac
     1861 ctgatgacag gtaaacgggt ggcaacagta cagaaagacg gacgaagggt ggagtttacg
     1921 gccacttccg tgtctgacct gaaaaaatat attgcagagc tggaagtgca gaccggcatg
     1981 acacagcgac gcaggggacc tgcaggattt tatgtatgaa aacgcccacc attcccaccc
     2041 ttctggggcc ggacggcatg acatcgctgc gcgaatatgc tggttatcac ggcggtggca
     2101 gcggatttgg tgggcagttg cgggcgtgga acccaccgag tgaaagtgtg gatgcagccc
     2161 tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga tctggtacgc aataacggct
     2221 atgccgccaa cgccatccag ctgcatcagg atcatatcgt cgggtctttt ttccggctca
     2281 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     2341 gcgaggttga agcggcatgg aaagaatttg ccgaggatga ctgctgctgc attgacgttg
     2401 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     2461 gagaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt
     2521 tccggatggt cagcccgaag cgcatcagca atccgaacaa taccggcgac agccggaact
     2581 gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg
     2641 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     2701 ggcgcgcctc gttcattcac gtctttgaac ccgtggagga cgggcagacc cgcggtgcaa
     2761 atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc
     2821 tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat tgaaagtgag ctggatacgc
     2881 agtcagcgat ggattttatt cttggcgcga acagtaatga gcagcgggac aagctggcgg
     2941 gctggattgg tgaaattgcc gcgtattacg ccgccgcgcc ggtccggctg ggaggcgcaa
     3001 aagtgccgca cctgatgccg ggtgactctc tgaacctgca gacggctcag gacacggata
     3061 acggctactc cgtgtttgag cagtcactgc tgcggtatat cgctgccgga ctgggtgtct
     3121 cgtatgagca gctttcccgg aattacgccc agatgagcta ctccacggca cgggccagtg
     3181 cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga
     3241 gccagatgtt tctgtgctgg ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt
     3301 caaaagcgcg tttcagcttt caggaagccc gcagcgcctg ggggaactgt gactggatag
     3361 gctccggtcg tatggccatc gatggtctga aagaagtaca ggaagcggtg atgctgatag
     3421 aagccggact gagtacctac gagaaagagt gcgcaaaacg cggtgacgac tatcaggaaa
     3481 tttttgccca gcaggtccgt gaaacgatgg agcgccgcgc agccggtctt aaaccgcccg
     3541 cctgggcggc tgcggcattt gaatccggac tgcgacaatc aacagaggag gagaagagtg
     3601 acagcagagc tgcgtaatct cccgcatatt gccagcatgg cttttaatga gccgctgatg
     3661 cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg caggccagct tgggatcagc
     3721 cgcctgacgg atgcggtgtc cggcgacagc ctgactgccc aggaggcact cgcgacgctg
     3781 gcattatccg gtgatgatga cggaccacga caggcccgca gttatcaggt catgaacggc
     3841 atcgccgtgc tgccggtgtc cggcacgctg gtcagccgga cgcgggcgct gcagccgtac
     3901 tcggggatga ccggttacaa cggcattatc gcccgtctgc aacaggctgc cagcgatccg
     3961 atggtggacg gcattctgct cgatatggac acgcccggcg ggatggtggc gggggcattt
     4021 gactgcgctg acatcatcgc ccgtgtgcgt gacata
//
LOCUS       DAZFRJ010000285         4033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000285 DAZFRJ010000000
VERSION     DAZFRJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.287"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..219
                     /locus_tag="ACHKUN_004563"
     CDS             <1..219
                     /locus_tag="ACHKUN_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662678.1"
                     /translation="NLEGKRIGYVIKTENKETPFTVVDIDGPSGNVKTLDEGVTKMSL
                     VHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            318..2969
                     /gene="recE"
                     /locus_tag="ACHKUN_004564"
     CDS             318..2969
                     /gene="recE"
                     /locus_tag="ACHKUN_004564"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO6662679.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            2962..3771
                     /gene="recT"
                     /locus_tag="ACHKUN_004565"
     CDS             2962..3771
                     /gene="recT"
                     /locus_tag="ACHKUN_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO6662680.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            3828..4022
                     /gene="ralR"
                     /locus_tag="ACHKUN_004566"
     CDS             3828..4022
                     /gene="ralR"
                     /locus_tag="ACHKUN_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO6662681.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
BASE COUNT         1223 a          944 c          994 g          872 t
ORIGIN      
        1 aatctggaag gaaagcgtat tggctacgtg attaaaacag aaaataaaga aaccccattc
       61 actgtggttg atattgacgg tccatcaggc aacgtaaaaa cacttgatga aggtgtcaca
      121 aaaatgagtc tggttcacat cggaaagaat ctgcccgcag aaaaaaaagc cggatttctg
      181 gcaactctga ttgcaatgaa attaaaaggt gaaatctgaa aaaagaaagc ctgcacaacg
      241 tgcaggcctg agtgaagaac ctgggacatt tattcatcac tcgcagtaat tttaatctga
      301 gttgaggtta aaaaacaatg agcacaaaac cactcttcct tttacggaaa gcgaaaaaat
      361 catccggtga acctgacgtc gtcctgtggg caagcgacga ttttgaatca acctgtgcca
      421 ctctggacta cctgatcgtt aagtcaggta aaaaactgag caactatttt aaagctgttg
      481 ccacaaattt tcctgtcgtt aatgaccttc cccctgaagg tgagatcgat tttacctgga
      541 gtgaacgcta tcaactcagc aaagactcca tgacctggga actaaaaccg ggagcagcgc
      601 cagacgacgt tcaccatcag gaggatgcac cggaaaccga agaaccgacg ggaggccagg
      661 aagaaaacgc gcaggcagac gcccacgagg attgccagga ttgcgaagtc tctgtagcca
      721 ctttgcggtt cacacagcgt cttctgcaca tttttacgta tgcagccggg gatcgaaaat
      781 acctgcatca tgccacccgt gaacaacgca aacacattac tgctcttgag atggatcagg
      841 aaaacagcta tgtccagaat ctgctgttgg ccatacgcag catggcagaa ccgacaactc
      901 tggataatgc cgccctgctc cgcctgactg atgcaattaa ggcagtgttc tctatcacga
      961 aaaaacatca gccctatgaa tttaagaatt tcatttcagc ctggctggat accgaacaca
     1021 ttgatcgcgg tcttctgaca aaagaatggc gaaaagggaa tcgtgtttca cgcatcactc
     1081 gcacggcttc cggtgctaat gctggcggcg ggaacctcac cgatcgcggc gaaggtttcg
     1141 tccacgatct gacatcactg gcgcgcgatg tagccactgg cgtactggcc cgttcaatgg
     1201 acgtggacat ctataacctt catccggcac acgctaaacg cattgaggaa attatcgctg
     1261 aaaataaacc acccttttct gttttccgcg acaaattcat caccatgcct ggcgggctgg
     1321 attattcccg cgccatcgtg gttgcgtccg tgaaagaagc accaattggg atcgaggtca
     1381 tccccgcaca cgtcactgaa tatctgaaca aagtactgac tgaaaccgat catgccaacc
     1441 ctgatccgga aatcgtggat attgcctgcg gtcgctcctc ggccccgatg ccgcaacgag
     1501 taacagaaga aggaaaacag gacgatgaag aaaaaccaca accatctggc gcaatggcag
     1561 atgaacaggc aacggctgaa acagtggaac cgaatgcaac tgaacatcat cagaacacgc
     1621 agccgctgga tgctcagtca caggtaaatt ctgttgatgc gaaatatcag gaactgcggg
     1681 cagaactcca tgaagcccgg aaaaacattc catcaaaaaa tcctgtcgat gccgataaat
     1741 tgcttgctgc atcacgtggt gaatttgttg acggaattag cgacccgaac gatccgaaat
     1801 gggttaaggg gatccagact cgcgattctg tgtaccagaa ccagccagaa acggaaaaaa
     1861 tcagcccgga tgcgaaacaa cctgagccag tagtgcaaca ggaaccggaa atagtctgca
     1921 atgcctgcgg tcagactggc ggggataact gccctgactg tggtgcggtg atgggcgacg
     1981 caacatacca ggaaacattc ggtgaagaga atcaggttga agctaaggaa aaagatccgg
     2041 aggaaatgga aggcgctgaa catccgcaca atgagaatgc tggcagcgat ccgcatcgcg
     2101 attgcagtga tgaaactggc gaagtcgcag atcccgtaat cgtagaagac atagagccag
     2161 gtatttatta cggaatttcg aatgagaatt accacgcggg tcccggcgtc agtaagtctc
     2221 agctcgatga cattgctgat actccggcac tgtatttgtg gcgtaaaaat gcccccgtgg
     2281 acactacaaa gacaaaaacg ctcgatttag gaaccgcttt ccactgccgg gtacttgaac
     2341 cggaagaatt cagtaaccgc tttatcgtag cacctgaatt taaccgccgt acaaacgccg
     2401 gaaaagaaga agagaaagcg tttctgatgg aatgcgcaag cacaggaaaa acggttatca
     2461 ctgcggaaga aggccggaaa attgaactca tgtatcaacg cgttatggct ttgccgctgg
     2521 ggcaatggct tgttgaaagc gccggacacg ctgaatcatc aatttactgg gaagatccgg
     2581 aaacaggaat tttgtgtcgg tgccgtccgg acaaaattat tcctgaattt cactggatca
     2641 tggacgtgaa aaccacagcg gatattcaac gattcaaaac ggcttattac gactaccgct
     2701 atcacgttca ggatgcattc tacagtgacg gttatgaagc acagtttggc gtgctgccaa
     2761 ctttcgtttt tctggttgcc agcacaactg ttgaatgcgg acgttacccg gttgagattt
     2821 tcatgatggg cgaagaagca aaactggcag gccagcagga atatcaccgc aatctgagga
     2881 ccctggctga ctgcctgaat accgatgaat ggccagctat taaaacgtta tcactgcccc
     2941 gctgggctaa ggagtatgca aatgactaag caaccaccta tcgcaaaagc cgatctgcaa
     3001 aaaacccagg gaaaccgtgc accagcagca gtaaaaaata acgacgtgat cagctttatt
     3061 aatcagccat caatgaaaga gcaactggca gcagctctcc cacgccatat gacggctgaa
     3121 cgaatgatac gtatcgccac cacagaaatt cgtaaggttc cggcgctagg aaactgtgac
     3181 accatgagtt ttgtcagtgc gatcgttcag tgttcacagc tcggccttga gccaggtagc
     3241 gccctcggcc acgcatattt actgcctttt ggtaataaaa acgaaaagag cggtaaaaag
     3301 aacgttcagc taatcattgg ttatcgcggc atgattgatc tggctcgccg ttctggtcaa
     3361 atcgccagcc tgtcagcccg tgttgtccgt gaaggtgacg agtttagttt tgaatttggc
     3421 cttgatgaaa agttaataca ccgcccggga gaaaacgaag atgcaccagt gacccacgtc
     3481 tatgctgtcg caagactgaa agacggaggg actcagtttg aagttatgac gcgcaaacag
     3541 attgaactgg tgcgcagcca gagtaaggct ggtaataacg ggccatgggt aactcactgg
     3601 gaagaaatgg caaaaaaaac ggctattcgt cgcctgttta aatacctgcc tgtctcaatt
     3661 gaaatccagc gtgcagtatc aatggatgaa aaggaaccac tgacaatcga tccggcagac
     3721 tcctctgtat taaccgggga atacagtgta atcgataatt cagaagaata attcagcctg
     3781 gcggtgtaat gcaccgccaa cgtgagacag tttttatgac aaaaattatg agatatgacg
     3841 atgttaaacc atgtccgttt tgtggttgtc catctgttac agtgaaagca atttcaggat
     3901 attaccgggc aaaatgcaac ggatgcgaat cccgaactgg ctatggtgga agtgaaaaag
     3961 aagcactcga aagatggaat aaacgaacta ctggaaataa taatggaggt gttcatgtat
     4021 aaaataactg cca
//
LOCUS       DAZFRJ010000286         3913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000286 DAZFRJ010000000
VERSION     DAZFRJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.288"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..207
                     /locus_tag="ACHKUN_004567"
     CDS             <1..207
                     /locus_tag="ACHKUN_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6662682.1"
                     /translation="LYLKPYGVLDSKGFGVFLAMICATFIISIISYNFIEKPSVSIGH
                     AIARRMQKKSDVSSIKTESVREAP"
     gene            complement(452..1609)
                     /gene="intS"
                     /locus_tag="ACHKUN_004568"
     CDS             complement(452..1609)
                     /gene="intS"
                     /locus_tag="ACHKUN_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO6662683.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1771..1845)
                     /locus_tag="ACHKUN_004569"
     tRNA            complement(1771..1845)
                     /locus_tag="ACHKUN_004569"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1810..1812),aa:Arg,seq:cct)
     gene            complement(1921..2853)
                     /gene="yfdC"
                     /locus_tag="ACHKUN_004570"
     CDS             complement(1921..2853)
                     /gene="yfdC"
                     /locus_tag="ACHKUN_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO6662684.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3147..3902
                     /gene="mlaA"
                     /locus_tag="ACHKUN_004571"
     CDS             3147..3902
                     /gene="mlaA"
                     /locus_tag="ACHKUN_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO6662685.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          951 a          936 c          928 g         1098 t
ORIGIN      
        1 ttatatctca aaccgtacgg ggtattggat agtaaaggat ttggtgtttt tctagctatg
       61 atttgcgcta cttttattat atcaatcata tcttataact ttattgaaaa accatcagta
      121 tccatagggc atgccatcgc tcgtagaatg caaaaaaaat cagatgtatc ttcaattaaa
      181 actgaatccg ttagagaagc accttgataa atagagcaat agccagatag cacctaaccg
      241 atttcgtact catagggttg tcactttacc aaactgagag gaaatacact gataacagtt
      301 ggttatgaag tactgggtga ggtggatgtg aatggtattt tgatgcattt cattaacctg
      361 tacgcctaag aaagcaaccc tgaaatctaa gacacacaag ctttgcactg gattgcaagg
      421 ctttgtgcac ttcgatagtg gttaaggtgg ttcactccac cttttcatca agccagtccg
      481 cccaccattg catcatttct ctgcgcttat cgagatactg agcatggttg taaataccgc
      541 gcacagatcc gccgttggca tgtgccagtt gcacttcaat agcgtcagca ggccattcgt
      601 gctcgttcat aatcgtgctg aattcatgcc tgaatccgtg accgctttcc agaccctcat
      661 agccgatttg tttgatcaca agtagcaccg cgttctcgca gattggcttc ttcttatcgt
      721 tgcgcccggc aaaaacaaat tctgatactg gtttggtgat ggagcttaac gtagtgagaa
      781 gttcaaccac ctggtccgac atcgggacaa catgaatttt gcgtcccttc atcacattgg
      841 cgtcaatggt gataatcctg ttttcaaaat cgacgttctt ccattgcatg gaacgaagct
      901 cttttgttct tagggctgtg tagcgtaaaa ccttggtggc aatgagcgat acgatacttc
      961 ctgaaaatgt tgccagtgct ttattgaatg ccgggatctg gtctgcagga agaaacggga
     1021 agttcttctt gcggtatccc ctcatggcgt cagcaaggtc aggtgccggg ttatatttag
     1081 ccctgccggt gacaatagcg taacggaaaa cctcgccgca tcttctgcgg gctttatttg
     1141 ctcgctccat tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct
     1201 caatatcctg aatttcaagg ccgccgatga taggcaaaat gtcgtcatca aacatttttg
     1261 caagttcagt cgcataccct actgaccata cttgcttctt gtgctcgtac cattccttgt
     1321 aaatcgcact aaaggaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc
     1381 caaccgagat gtctttcctc gcagtccatg ccttatccct tgcctcctgc aaagtcatta
     1441 gcggatattt tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc
     1501 ataccttttt cccggataca ggaacataaa ggtacaggcc attaccatcg agaaggcggt
     1561 atggtttttc tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc
     1621 cggagggtaa aattatttta tccacttttt acccgtcatg gggtgcggct gtcaacgatc
     1681 tgacgcgaac catgacgaac tgtgaatcta cggaaggctt gatattcagg ggattttgcg
     1741 gactggtacg gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa
     1801 ttagccctta ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta
     1861 tacccgctaa tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat
     1921 ttatgctggg tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc
     1981 ttctgcttta cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa
     2041 gataaaggtg ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa
     2101 atcgctccag tgcagcgtgc cgttaaacac cagatagagg atttcaacag aaccgacaac
     2161 gatgtgggtg gtgtcaccca gtgcaataag ccaggtcatc aaaataatca ccacaatctt
     2221 tgccgcaccc gctgcaggaa acatccaaac catagtggcg atcagccagc cggaaatgat
     2281 cgcgttggca aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa
     2341 tgcatcgcga gtttcttcat tgaagatagg catatattca aatgcccatg cagcaatacc
     2401 tgtcccgaga atattaccca gcagcacgac gccccataat cgcataagta agccgacgtt
     2461 gctcattgtc ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa
     2521 ttgctggcgg gccataatga cgataataaa accaaaggta taaccgagat tctccagtaa
     2581 gaagctgcct ggcactcctt ccagttcgac atgaaatatc ccttttgcca gtagcgaagc
     2641 gcccatcgac agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttccag
     2701 ctctttttca ccatcctggc ggatatgctc atgaattgcc atcgcccggg aggggagtcg
     2761 gtcttcatct atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc
     2821 gtcgctgtgt tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat
     2881 agctaaagcg tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa
     2941 aaggctgcgt ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta
     3001 ccaggctatg atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca
     3061 atcgcccaca cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata
     3121 agccataaaa aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa
     3181 ctacgcttct ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt
     3241 tagaagggtt caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg
     3301 ttcgaccggt cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aatggtttga
     3361 gcaactttac tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg
     3421 acccttatca ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg
     3481 gcggctttat tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc
     3541 gcttcggtag tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt
     3601 tctacggtag cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg
     3661 ttctttcctg gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa
     3721 cccgcgctca gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta
     3781 tggtgcgcga agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac
     3841 cgcaggaaaa tccgaacgca caagcgattc aggatgattt aaaagatatt gattctgaat
     3901 aagaaacaaa taa
//
LOCUS       DAZFRJ010000287         3866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000287 DAZFRJ010000000
VERSION     DAZFRJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.289"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..227)
                     /locus_tag="ACHKUN_004572"
     CDS             complement(<1..227)
                     /locus_tag="ACHKUN_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO6662686.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANT"
     gene            complement(368..1009)
                     /locus_tag="ACHKUN_004573"
     CDS             complement(368..1009)
                     /locus_tag="ACHKUN_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO6662687.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            1704..2735
                     /gene="repA"
                     /locus_tag="ACHKUN_004574"
     CDS             1704..2735
                     /gene="repA"
                     /locus_tag="ACHKUN_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO6662688.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1047 a          952 c         1005 g          862 t
ORIGIN      
        1 gtatttgcta ccggtatgtc cgtattggtg gccagcagtt ttcgcagagt cgtaaactct
       61 tctgccattc ctctgaatgc gctcatctga cgcgcagatt caagccagtt cagcaggtaa
      121 cgccggcgac gccgttcgtt gttgatactg tccggggcgg tggagagtgc ttcgagcata
      181 ttctccagta acagaatgac agggttttcc agggatagaa tggtcataaa ggcgtcctga
      241 aaaaatgccc gacagccgct ctccgtacac cgtaaggtgt caccggtgcc gggcattgtg
      301 gaaacagaag gaaaaaaaat cccggccata aaggccggga aaagtcgtca aattaagaca
      361 gcaattttca tgtcaacgct ccgtcatcag ataccaggtg caggagcagg gcgatcctcg
      421 actccggcag aggcggtgca tcaagccgga ggaggtactg gtaacgcgct tttgtcagcc
      481 agagggtgga ggcagcatcc agctcctccc tggctgccgg attgagcgca ggatccgggt
      541 acactttcat caaagactga acgatatcct ccctgacggt tttgatttca ccggccattt
      601 tcacgaagtt cagaaaggca ctgtgacggc gaaggtgagt gaagctgtgg cgttcggggt
      661 ctgctttcag gaaaaagtaa ccgggaaaca cggcgtgtat atgtcgacgg taaccgcaga
      721 ctttatccgt gcggcggact tttttcagta tcaggggtgt ccagggaagg acgtgttgtt
      781 cggaaagcca ggaaaagaga tgttcgcggt ttttgccggc agggatgtac tgagccagat
      841 accagttgcg ttgatgtaat tctctgatat tcaaaatccg gaacgctccg cgaaaatcga
      901 tccccgcaga ggggcttcga tgttgttgtg aacggcgaat acataaagca tgtgtcggaa
      961 acagcggctg atcctcgttc agcccggcct gcagttctga caggcacatg atttacgcac
     1021 tcttgtaagc cttgtggacg gtttcgaggc cggcgaaaac cgacaaaacc tgcaaacagg
     1081 attaatggct ggcaaacata ttacataagg atatatctgt ggcaagagcg aagataagta
     1141 gttaaataga tcgttatatt tgataaagca gcatattacg ctgggttgtg tgaggtgttt
     1201 ggatgattag atcgtaatta caagatcgat tcatccaaaa aacatgaaaa acacagcata
     1261 aaagatcgtt tgtggaattg tctatttttt gacacagttc aaaaaaacga cgttttttag
     1321 gtggttaatg agaggcctgg taaatcagag aaatcgtgtt ggcttttcaa agcggtggaa
     1381 aaggggtata ttgcggatcg ttatgcagtg gcttttggga tcgtcaggat ccgggaagtc
     1441 agaaaatggc aggatgcgcc ataaggcatt caggacgtat ggcagaaacg acggcagttt
     1501 gccggtgccg gaaggctgaa aaaagtttca gaaggccata aaaggaaaac ccccataatc
     1561 ttctcatcga acttggcgga acgttagaag attatggggg cctcacagaa tacgggataa
     1621 gtatatatga aaccgtgtca gagattcaac ccttcgcagt gtattaacgc acggcattct
     1681 gtttccgcca taagcgacag cttgtggcag gtctgaagaa tactccatat aacgcagtac
     1741 actggagtca gttagcaccc gaagagcaga tccgtttctg ggaagactat gaagcgggaa
     1801 gggcgacctc tttcctggtt gaaccggaaa ggaagcgcac caggcgccgt cgtggcgaac
     1861 actccactaa acccaaatgc gaaaatccgt cctggtatcg tcctgcgcgc tataaggagc
     1921 tgagcgggca gctcgggcac gcctacaacc gtctggtgaa aaaggccccg gtgaccggcg
     1981 agcagagcct gcgtatgcgt atgtcgcggc atccttttta cgtgcagaaa cggacgttcg
     2041 tcggtcgcaa atatgctttc cggccggaaa aacaacgcct cctcgatgcc atctggccgg
     2101 ttctggtcag cttcagtgat gccggcacac acactgtggg gatgagtgtc actcgcctgg
     2161 ctaaagaaat cagtccgaaa gacagtaagg gaaatgttat tccggaactg gaagtgacgg
     2221 tctcccgtct ttcccgtctg ctggccgaac aggtgcgttt tggtgtgctg ggtgtttcag
     2281 aggaaacgat gtgggaccgt gaaacccgcc agcgtctgcc gcgctatgtc tggataacac
     2341 cggctggctg gcagatgctg ggcgtcgaca tggtaaaact tcacgaacag cagcagaaac
     2401 gactgcgtga aagtgaaatc cgccagcagc tcattcggga aggtgttctg cgtgaggatg
     2461 aagatatctc cgtacatgcg gccagaaaac gctggtatct gcagcgcagt caggatgcgc
     2521 tgaaacaccg tcgtgcgaaa gcagcagcca gtaagcgcgc cagacgcctg aagaaactgc
     2581 ctgccgacca gcagattcat gagatggcag agtatctcag gaagcgtctg ccgccggatg
     2641 aagcctattt ttgttccgat gaccatctga agcgactggc catcagggag ttgcgtcagc
     2701 ttgaactgac gctggctgcc ccgccaccgc actagacagc accattccct cagcactgaa
     2761 tcatcgccag cccctccggg gctttcggcg caggtatcgc tcagcccaaa atccgcagta
     2821 atcaccttaa atcccctcag aggggcatat ttgctcataa aaccacgcat cagtcatcag
     2881 aacgtggcca cgttgtttca gttatccaca taaatccgca aacaaagaac tttaagaagc
     2941 tgcaaacctg aaacagcaaa cctgcaatat agtcttaacc ccattattta atcccctgcg
     3001 ttgcttcgcc gcagcgaaag tctttatctc tgaaaccact gtgaacaaac acaaagaggc
     3061 cttcgcttgc agcggccaag gccgcgccgc tcagaatcta aaagcacctc ccacgctgac
     3121 gcgcgggccc cgaacctcac cgttctggaa ccacaacaaa aaaacatcag gaataaaaac
     3181 accacacaaa cgcagcaccg tgcccacccc tcataactga aaagcgccgc cgccccggcc
     3241 caaagggccg gaacagagtc gcttttaatt atgaatgttg taactaaaca tcatcatcgc
     3301 tttaagaccc catttgtcta gcagggcgat ggcccactgc gtgaaccatc accctgcaca
     3361 cagggtattt tatgtgcaat tgtcaaccac tgaatcggaa ccccagaggg gcccccacga
     3421 ttcagaagtt gacaatttaa gcacagggac tacatgagaa aaatcgtggt tcgtctgtca
     3481 tacacgcgcg taagcggcac tgatagccct cttacgcgga aatacgccca taagcgctaa
     3541 cttaagggtt gaaccgacgc cacggtgtct cgttaagact tcaattgcgt tttgtaggca
     3601 gtcagggata ctgtcccatg aatggccacc tgtaagctcc agataaccat ttttgttatt
     3661 ctccacaacg aattccgcat accggaaatc agcgctactt ctggggagga atggccttaa
     3721 gagtgataaa gattatatgc ttttcataga gagactcatc tatgccggta acactgaaaa
     3781 atgtattgtg ttccaagaag tttttttgag tgaacatatt ttcgctcaca acaatcgaat
     3841 tattcaaagc cacaatttta aaaaat
//
LOCUS       DAZFRJ010000288         3847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000288 DAZFRJ010000000
VERSION     DAZFRJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.290"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..85)
                     /locus_tag="ACHKUN_004575"
     CDS             complement(<1..85)
                     /locus_tag="ACHKUN_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFO6662689.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMA"
     gene            complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHKUN_004576"
     CDS             complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHKUN_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6662690.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHKUN_004577"
     CDS             1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHKUN_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO6662691.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHKUN_004578"
     CDS             complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHKUN_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO6662692.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
BASE COUNT         1080 a         1023 c          939 g          805 t
ORIGIN      
        1 gagccataaa ccgctcacgg ttataggttt tttgatcgct taagaaagta ccatccatgt
       61 ctaccgcaat taatttaatg ctcatcaact attctccatc gccgtctgcg ttttggtatc
      121 gggtttggcg acggctttcg cgacaatagc cgcgagaata accagcgcca gtacaaccag
      181 cattgcacta cgtaatccat aatgttcgcc gagatagccc agcagcggcg gcccgacgag
      241 gaaagccaga taaccggtcg ttgctaccac actgacgcgg gttggtgcat cggggccggt
      301 atcgctggcg gcagaaatgg tcagcgggaa gcccagcgag gcacccagtc cccagagtac
      361 aacagacacc ccagcgaccc aggcgctatc gacaaaaata atcagcccaa tacccaacgc
      421 tcccattagt gcactggccc gaaccacggc aacgcgactg taacggtcga tgaaccaacc
      481 gccggtaaag cgtccaacag tcatccccag ggtaaaaccg gcataaatca gcgagccgga
      541 agtagggcta aaaccgtgac cgtcaaccat taataagggt aaccagtcgt tggcagaacc
      601 ttcggcaaag gccatcgcca gcaccacaac accaatcagc agcaactgga tatcgcgata
      661 aaaaggtacg cctttttcac catgctgggt gccatcggca gcatttttgc ccgtaccgtc
      721 agggattgcc tgaatggcga tataaatagg cgcgattcct accagcgccg ccaataaaat
      781 gtgcaccgtt gccggaacgc caaaggccgt cagtgccatc ccgacaccag cgcctgccag
      841 cgtgcccagg ctataaaaac cgtgcatcat cggcaaaacc gttttattca tttctcgctc
      901 aacggcggca ccttcgacgt ttatcgccac ttccgcagaa ccaaaacttg ccccaaagac
      961 gccgaggcca accgcgaaga gcaggggtga tgtcagccag agtgccagac ttaatatcat
     1021 catcccgatc aatgcgcagg acatcgtgac caggatgaca ttacgtgtcc caaagcgttt
     1081 cactaaccac gccgagcaga gaataccgct catcgaaccg atcgacagac caaagagaac
     1141 accgcccatt tcagcgatcg agacagagag aatatcgcgg atagcaggcg tacgggttgc
     1201 ccaggacgcc attaacaggc ccggcaaaaa gaagaacata aacagcgccc aggttcggcg
     1261 tttcaatgca ttacgtgaag aatttacggt catagatcac gtcaaaataa gaagagggaa
     1321 gacaacatta gcaaggttgt gtacatttgt acacaattgc agacagagga aatgacatgc
     1381 gtcgcgctaa cgatccgcaa cggcgagaaa aaattatcca ggccacgctg gaggcggtga
     1441 aactttacgg aatacatgct gttacgcacc gcaaaattgc tacccttgcc ggggtaccgt
     1501 tgggatcgat gacctactat ttttcaggaa ttgatgagtt gttgctggag gcgttcagca
     1561 gttttactga gattatgtcc cggcaatatc aggcattttt tagcgatgtt agtgatgctc
     1621 aaggcgcatg ccaggctatc accgatatga tctacagctc acaggttgca acgccggata
     1681 acatggagct gatgtaccag ctctacgcgc tggctagccg aaaaccgcta ttaaaaacgg
     1741 taatgcaaaa ctggatgcag cgcagtcagc aaacgctcga acaatggttt gaacccggaa
     1801 ccgcccgcgc gcttgatgcg tttattgagg ggatgacgct gcattttgtc accgaccgta
     1861 agccgctatc gcgcgaggag attttgagga tggttgagag ggttgcaggg tagtagataa
     1921 attttagata acaaaaaacc catcaacctt gaaccgaaat ggcggggttg atgggctcca
     1981 caaaatgggg acatcaaaga aaagcagtgg cactaattaa gactgatgcc ctgcggaaaa
     2041 gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac ttcagtttta tcctaatcct
     2101 ggccatacca tgacgatgat tgtccctgcc agcgtcagca ggacgttggc gattgcgtag
     2161 gtgcccgcat agcccagcgc agggatgtta ctgcgagctg tatcactgat gatctccatt
     2221 gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca acagtgcgcg gttcattcgc
     2281 aatacataag caccgaacaa gaaacagata accacgggca caagactgac aattaatccg
     2341 gcaatcaaca tctgaccgcc aatcgcgccc aggccgttat taataccgct accggcgctc
     2401 agaccaacgc ctgccataaa caccatcaag ccgaactctt tcaccatgct taatgcacct
     2461 tgcggaatgt aaccgaaggt cgggtggtta gcacgcataa agcccagcat aattccggcg
     2521 aataacaacc cggcagcgtt ccccatgccg aaactgaatg tgctgaactg gaaggtgatc
     2581 atcccgatca tcagcccaat aacaaagaag gcgcaaaatg ccagcaggtc agtgacctgg
     2641 ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt ttacgcgacg ggcatcaccg
     2701 ctgacttgta aaacgtcacc tttgttaagc acgacgttgt catctatcgg catctcaatc
     2761 tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg tcaacttcag ttgtgcgaga
     2821 cgtttaccta cagcgttatg gtttttaacg accacttctt cagtgacgat acgcatgtcg
     2881 agaaggtcac gatcgaaaac ttctttaccg ttacggaagc tgggatcgag tcgggcatgg
     2941 gcgtcgggat agcctaccaa cgctatttca tcgcccattt gtagcacggc atcaccgtct
     3001 ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt agcagccggt ttgtcgataa
     3061 atacccagtt cacgcagatt tttgccgtcg gtccaggcca ccagctccgg gccgacgcga
     3121 taggcgcgga tcaccggtaa ataaacctta cggttggcat cagtgtccag gccacgttcg
     3181 cgggcgattt gctgggcgct ggtctgtaag tcctgatgct gcaatttcgg caagtaacgc
     3241 gcaccaacaa tcaaactcac cagaccgatt aaataggtta aggcataccc gaggctcaga
     3301 ttatccagtg ccagtgagag ctgcctgctt tccatgccgg aatgacgcag tgtatcgcca
     3361 gcaccgacca gaaccggtgt cgacgtcata gagcctgcta acataccggc cgtcaggcca
     3421 atatcccagc caaacagctt acctaaccct aaggcgatca ccagcgcact gccaaccatc
     3481 accagtgcta acattaggta atttttccca tcgcgaaaaa aaatggaaaa aaagttcggt
     3541 ccggcttcga ccccgacgca gaaaataaac agcataaagc caagattaag cgcatcggtg
     3601 ttaatgctga aatgttgttg gcctaataac agcgatacga ctaaaacgcc aatggaatta
     3661 cccagttgga tcgaaccaag tcgtaacttt ccgagacata gcccaagcgc gaggaccaca
     3721 aataataaca gaatgtaatt cccatttaac aattcggcga cgtttatatt cacggaggct
     3781 aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa tttacgataa tgttttttac
     3841 cagaatt
//
LOCUS       DAZFRJ010000289         3845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000289 DAZFRJ010000000
VERSION     DAZFRJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.291"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..186
                     /locus_tag="ACHKUN_004579"
     CDS             <1..186
                     /locus_tag="ACHKUN_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO6662693.1"
                     /translation="EDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQRD
                     NTLLMIGKVIDYKEDVI"
     gene            244..822
                     /gene="espA"
                     /locus_tag="ACHKUN_004580"
     CDS             244..822
                     /gene="espA"
                     /locus_tag="ACHKUN_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO6662694.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            835..1977
                     /locus_tag="ACHKUN_004581"
     CDS             835..1977
                     /locus_tag="ACHKUN_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO6662695.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            1998..2942
                     /gene="espB"
                     /locus_tag="ACHKUN_004582"
     CDS             1998..2942
                     /gene="espB"
                     /locus_tag="ACHKUN_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO6662696.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            2949..3356
                     /gene="cesD2"
                     /locus_tag="ACHKUN_004583"
     CDS             2949..3356
                     /gene="cesD2"
                     /locus_tag="ACHKUN_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO6662697.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3393..3614
                     /gene="escF"
                     /locus_tag="ACHKUN_004584"
     CDS             3393..3614
                     /gene="escF"
                     /locus_tag="ACHKUN_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO6662698.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            3620..>3845
                     /locus_tag="ACHKUN_004585"
     CDS             3620..>3845
                     /locus_tag="ACHKUN_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO6662699.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQ"
BASE COUNT         1143 a          737 c          900 g         1065 t
ORIGIN      
        1 gaagactggc tcctggaaag cccttctcgg gtatcgattg tcgaagataa acatgtttat
       61 tatttccacc tattgaaaga cttttttgca tcattacctg acgcttgttt tattgatagg
      121 gaacagagag ataatacatt attaatgatt ggtaaagtaa ttgattataa ggaggatgtt
      181 atttgatatt ggttttttaa tcgtttttgg tcttgctaag aaagattatt aagaggtata
      241 tacatggata catcaactgc aacatcagtt gctagtgcga acgcgagtac ttcgacatcg
      301 acagtctatg acttaggcag tatgtcgaaa gacgaagtag ttcagctatt taataaagtc
      361 ggtgtttttc aggctgcgct tctcatgttt gcctatatgt atcaggcaca aagcgatctg
      421 tcgattgcaa agtttgctga tatgaatgag gcatctaagg agtcaaccac agcccaaaaa
      481 atggctaatc ttgtggatgc taaaattgct gatgttcaga gtagttctga caagaataag
      541 aaagccaaac ttcctcaaga agtgattgac tatataaatg atcctcgcaa tgacattaca
      601 gtaagtggta ttagcgatct aaatgctgaa ttaggcgctg gtgatttgca aacggtgaag
      661 gccgctattt cggccaaatc gaataacttg accacggtag tgaataatag ccagcttgaa
      721 atacagcaaa tgtcaaatac gttaaaccta ttaacgagtg cacgttctga tattcagtca
      781 ctgcaataca gaactatttc agcaatatcc cttggtaaat aaccggagat aactatgctt
      841 aatgtaaata gcgatatcca gtctatgagg tctggggcca gcgctgctac ggctacttca
      901 ggtattaatc agcctgaagt gacttccgcg ctggatttac aactggttaa atccacggcc
      961 ccatcagcaa gttggacaga aagtaccgct ttaccaacgc caccggcagg ccactcatta
     1021 gtgacgccct ctgttgctga ggatgtcctg agtaaactgt ttggtggtat tagtggtgaa
     1081 gttaggagtc gaacagaggg aaccgaacca cagcgcagca ctcagaatgc aagctctggc
     1141 tatccttatc tttctcaggt gaataacgtt gaccctcagg ccatgatgat gatggtcacc
     1201 ctgttatccc tggatgcttc agcgcagaaa gttgcgagta tgaaaaactc caacgagatc
     1261 tacacggatg ggcaaaataa ggcgctggat aataaaacgc tggagtttaa aaagcagctc
     1321 gaagaacaac agaaagccga agagaaagca caaaaaagta aaattcttgg tcaggtcttt
     1381 ggttggttgg gcgtcgcggt aactgctatt gcagctattt ttaacccggc actctgggct
     1441 gttgttgccg ttagcgcaac agcaatggca ctgcaaactg cagttgatgt aatgggcgat
     1501 aaggccccgc aggcgttaaa gacagcagcg caagtctttg gcggagtatc tattgctgca
     1561 agtattctga cagccggcat tggcggggtg tcttcactta tgtctaaagt tggcgatgtc
     1621 gctaacaaag ttggttcaaa catcgtgaaa acagttacta aactggccga tgtatttgtt
     1681 gagaacgttg tttcaaaagt tgcggcaacg gcaaatggat ttacgacctc tgcacgttct
     1741 attgggacaa ctgtgctgaa taatgattct gcgcaataca gcatattggc tcagttatct
     1801 gcttacgcgg tacaaaactt aactcgacag agtgaaaacc tgggtgagag tgcgaaggtt
     1861 gagctggata aagctgcagc cgagttgcga aaccaggcaa gctatttaca aaatgcttct
     1921 cagttaatat ccgactcagc acgagtaaat agccgtattg ttagtggtcg agtttaatta
     1981 ttaaagagaa tttaattatg aatactattg attatactaa tcaagtaatg acggttaatt
     2041 ctgtttcgga gaatactacc ggctctaatg caattaccgc atctgctatt aattcatctt
     2101 tgcttaccga tggtaaggtc gatgtttcta aactgatgct ggaaattcaa aaactcctgg
     2161 gcaagatggt gcgtatattg caggattacc aacagcaaca gttgtcgcag agctatcaga
     2221 tccaactggc cgtttttgag agccagaata aagccattga tgaaaaaaag gccgctgcaa
     2281 cagccgctct ggttggtggg gctatttcat cagtattggg gatcttaggc tcttttgcag
     2341 caattaacag tgctacgaaa ggcgcgagtg atattgctca aaaaaccgcc tctacatctt
     2401 ctaaggctat tgatgcggct tctgatactg cgactaaaac gttgactaag gcaacggaaa
     2461 gcgttgctga tgctgttgaa gatgcatcca gcgtgatgca gcaagcgatg actacagcaa
     2521 cgagagcggc cagccgtaca tccgacgttg ctgatgacat tgccgattct gctcagagag
     2581 cttctcagct ggctgaaaac gctgcagatg ccgctcagaa ggcaagtcgg gcaagccgct
     2641 ttatggctgc agtagataag attactggct ctacaccatt tattgccgtt accagtcttg
     2701 ccgaaggcac gaagacattg ccaacaacgg tatctgaatc agtcaaatct aaccatgaga
     2761 ttagcgaaca gcgttataag tctgtggaga acttccagca gggtaatttg gatctgtata
     2821 agcaagaagt tcgcagagcg caggatgata tcgctagccg tctgcgtgat atgacaacag
     2881 ccgctcgcga tctcactgat cttcagaatc gtatgggtca atcggttcgc ttagctgggt
     2941 aattgatcat ggtcgatacg tttaatgatg aagtgcttaa tcactatctt gaacaaaaag
     3001 ggtacacaat acagaaggag tttctttgtg gcagtgcctt ttttatcgga tggcggattg
     3061 agacctcttt tttctcatta gcgtacagac ttgatgaaca agaactgatt ttgtgctctt
     3121 ttgaagcacg taaccaaaca gggcttaacg gccctgtttt atcactgact cgcttgctcg
     3181 aagagttgta ccaccatttt tcgggtatta agaaaatcag tgcgatgaaa tctaagattg
     3241 gttcagattc agaacgtcaa aagcgcgaag agttgtttaa ttacttcatc agaaagggcg
     3301 ctgtgcagca agaaacagaa gacggaattt ggttcgtaat gaatgtaaat agttagaaat
     3361 ttttatttct attaactatt gaggaaaatt taatgaattt atctgaaatt actcaacaaa
     3421 tgggtgaagt aggtaaaacg ctgagtgatt ctgtgccaga gttacttaat agcaccgatt
     3481 tggttaatga ccctgaaaaa atgttagagc tgcagttcgc ggttcagcaa tattctgctt
     3541 atgttaacgt agaaagtgga atgctgaaaa cgataaaaga tttggtctca accatttcca
     3601 accgtagctt ttaattttta tggttaatga tgtttctgcc aacaagatac tggtgtgggc
     3661 ggctgtagct gcggcaaatc ataagcttcc caaatatgca gaatctatcc tgaatgtatt
     3721 gccgcaaatt atacccgata aaaaagatat cgcacattta gaatttatta ttttatatgg
     3781 attaaataga aaaaatgatg cggtaaaggc tctggaggac tttatggatg atgagacaag
     3841 ccagt
//
LOCUS       DAZFRJ010000290         3837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000290 DAZFRJ010000000
VERSION     DAZFRJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.292"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..952)
                     /locus_tag="ACHKUN_004586"
     CDS             complement(<1..952)
                     /locus_tag="ACHKUN_004586"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO6662700.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYK"
     gene            complement(971..2008)
                     /locus_tag="ACHKUN_004587"
     CDS             complement(971..2008)
                     /locus_tag="ACHKUN_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO6662701.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            2281..3513
                     /locus_tag="ACHKUN_004588"
     CDS             2281..3513
                     /locus_tag="ACHKUN_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6662702.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            3516..>3837
                     /locus_tag="ACHKUN_004589"
     CDS             3516..>3837
                     /locus_tag="ACHKUN_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO6662703.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDA"
BASE COUNT         1137 a          749 c          725 g         1226 t
ORIGIN      
        1 gtttatatac accatgctgt ttagttagtt tattaaggac agcgagattt ttgttgtatt
       61 ctcgcgtatt tttggcaggg taaccaaaaa ggtcactctt ttcaggacaa gaatggctta
      121 cacctgattt agccttaatt accgaataac taccaattga tatgtgaccg gcgattccga
      181 cttgaccatg tgtaataaca tggtcgccta aaactacgtg tcctgaaaaa ccgcattgtg
      241 aaacaatcag gcatttatta ccgattatgc agtcatgtcc aatctgcact tgattatcaa
      301 tacgagtgcc ttggcctata atcgtatctc caagtgtccc tcggtcaatg gtgttgttac
      361 aaccgatttc aacatcatca cctatgatta ctctaccgat gctatcaact cggacatatg
      421 aatcacgttc atccgacata tattcaaaac tataattacc gatggagtta ttagaatcta
      481 tgataacgtc attgccaata atagtacctt ctttaatcac tgtattcgca tgaatggcaa
      541 cattatcacc tatggtcaca cagttcataa ttttaacacc aggcataaaa tggcagttca
      601 tacctatctg gcagtgttta ccaatgtata catcaggtat cgttgaagtg ttttcttgct
      661 caaacaaacg atacttatgt accttaaaat atcttaaaac tttgttaaga ttattcacgt
      721 tcaggtaatc gactacaatc tttgccttag cattagaact aatgatctct tccggtccga
      781 taattatatc cgcaccagtc gttggtatat gcaacaaatc tccaggtgtc ataacaatcg
      841 agagtccacc aactaaagga ctatggattg gccgaatggt atcaaccctg agtgatggat
      901 caccatataa agttccttgc atcaacgttt taatatctag tgcgctgaac ataggtttcc
      961 ctctgtttaa tcaaaagcga tagaaagcat gaacataaat attatcctga ttgagcacat
     1021 tatatttttc ttcatctctt ggatcataaa agtttttgtg ctctttttcc caggcatgtt
     1081 tatattcaat cttggctatc cagtcgtcac ctattttttg ttcataacct agagtggctt
     1141 ttcgaattgt tttgaaatag gttctgtttg cattgtattg caaactatct gcgttttctg
     1201 ttttcccgtg tccgaagcgg gtgcgtagat acagattacc tgcgttttca aaagcatatt
     1261 tgataattgg ctcgtagtaa gtttcggtat aaatactacg atcatttatt ttaccgtgat
     1321 taaattcatc gttatgcttt ttctggtaga aaaattcaat accaaactcc catgcaccaa
     1381 aacgtttata gggctgaaat agtatacttt gaccttcttc ttcgcgtacg ccccaggagc
     1441 tcttatcatt tcgactgtat aagtattcaa cgtttgaata aaaacctgcg ttccagtcag
     1501 agttaaagta cgtcaacgta gggcgaaaac tgttgtcaac agttacagta tgcaagcctg
     1561 taacataagg gctatctata tttccgtcgg tatacatcag gtcgtatacc agactgaagt
     1621 cgaaaccgtt accaatagtg aacgttttgt ttagtgatat atagttttgc agacttgttt
     1681 cattttcgtg atagcctttt tcttcttgcc ggcgatcgat agacttaagg ccatagattg
     1741 ctgcaaattg ccaattaggt tggctgaaat aggcctggaa aatctcgtgt gttttagtat
     1801 attcctttac tgttttacct aaatagttat cagtgctttt ggtctcatat tcaaatgtag
     1861 taccaacatg tccattaaag cccgcttgtt cttctgacca gtcagtgtcg gaggtaaagc
     1921 cgcgatcgtt tgccgcaaag gctcctgttg agcctaaaag gactgttgca attgaagccg
     1981 ataagacatt cttttttatt aaaaccataa atgagttcct tccaaaatat caatttaaga
     2041 tatgatttct gttattccat atagtcgtac ctcatgtact cctaaacact ctgcggcaat
     2101 aaaatatttt tttcctcaac aaattactgc aaatgagata tgcatcacta tttgcagtta
     2161 ttcatcatta aaatcttatt tgacagttga ttggccttga tttgtgttga taatggcgtg
     2221 aatgcttatg taggcgtaca gcatgtacta cttaaagata ttattcacga ggtaaatata
     2281 atgagacttt caaatatcgt cacagttata attcttgcaa tttccagtac gctaacaccc
     2341 caggcgatgg cagaaaaatt gattcctgaa actgatgctg agttattaat ctggtctgat
     2401 gccacaagcg cggaatatat gaaatatgca gcaacggaat tcaataaaga ttttggctac
     2461 aaggtcaagt ttacatttcg caatatagcg ccaatggatg cagcatcaag aattatgcag
     2521 gatgggggta cgactcgtgt agctgatgta gctgaaattg aacatgatac cctggggcgg
     2581 ttagtcgttg ctggcggggt tatggaaaac atggtctcag ctgagcggat taaaaaaaca
     2641 tttattccag gcgcagtatc ggcagctaca tataataaca tcagctatgg ttttcctgta
     2701 agtttcgcaa cgctggcgct tttctataat aaggatttgt taaacaccgc accaaaaaca
     2761 ttcgaagaaa tcaatacttt cagtgaaaag tttaataatt catccgagca taaatatgct
     2821 ctgctatggg atgtacaaaa ttattatgtt tcacgtatgt ttattacctt gtatggtgcc
     2881 aacgaattcg gtaaaaccgg taacgatcct aaagctctag gcatcgcttc atctgaagcg
     2941 aagaaagggc tagagacaat gaaacgctta aagaaagcga atccttctaa tcctcttgat
     3001 atgggtaatc cacaagttct aagaggtctg tttaatgaag gaaaagttgc tgctgtaatc
     3061 gacggacctt ggtccataca aggttacatt gacagcggaa tcaattttgg cgtgacacgc
     3121 atcccaacat tagatggtca tcagcctcgc acattttcaa cagtacgact ggccgttgta
     3181 agctcattta ccgaatatcc tcatgccgct gaattattcg ctgactacct gacaacggat
     3241 aagatgttga tgaagcgtta tgaaatgacg aatctcatcc caccaatcga ttcacttatg
     3301 aacaaaatta gccagacagg tagtgaagct ataaaagcaa ttattgctca agctaattat
     3361 tctgacgcaa tgccatcaat accggaaatg tcttatctat ggtctccaat gactaatgct
     3421 attttggcta cctgggttga gaataaaaca ccggatgaag ttttaaatca tgcacaaaca
     3481 attattgaag aacaactttc gcttcaggag taattgtgaa tacacaacaa ctgaaaatga
     3541 agagtgcacc tgtgctacct atttcctgtt tgattatggg cgggactcag ttatcacgtc
     3601 attattatgt gaaaggcggg atattctttg caatacaagt atgctttttg ctttatcttt
     3661 ccgatattgt gcatacatta ataggtttgt tcacattagg tgatgtcgcg caaattcgaa
     3721 aagggttgac tgttattcag ggtgacaatt ctatatttat gcttgtagag ggcgtgatag
     3781 cgaccattat tgttggctta ttcgccacta tttacatcct taatattaaa gatgcgc
//
LOCUS       DAZFRJ010000291         3822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000291 DAZFRJ010000000
VERSION     DAZFRJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.293"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..490
                     /gene="ykgH"
                     /locus_tag="ACHKUN_004590"
     CDS             <1..490
                     /gene="ykgH"
                     /locus_tag="ACHKUN_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO6662704.1"
                     /translation="PGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIKL
                     EWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIYL
                     TRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQN
                     SG"
     gene            complement(736..1431)
                     /locus_tag="ACHKUN_004591"
     CDS             complement(736..1431)
                     /locus_tag="ACHKUN_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6662705.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1424..2851)
                     /locus_tag="ACHKUN_004592"
     CDS             complement(1424..2851)
                     /locus_tag="ACHKUN_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6662706.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2862..3581)
                     /locus_tag="ACHKUN_004593"
     CDS             complement(2862..3581)
                     /locus_tag="ACHKUN_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6662707.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          992 a         1023 c          806 g         1001 t
ORIGIN      
        1 accaggcata cttgttaact gtgatagcaa taataacttt gcctgtcaga ccacaatgac
       61 tgaagatgtt atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag
      121 agaaataaag ctggaatggt caggagataa gagagattta ccaactgctg aagcggaaat
      181 atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca
      241 agttctcgat gagatacaaa caccttcagc aattaacagt gagctttata caaaaatgat
      301 atacctgacc aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc
      361 accagaaata aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag
      421 cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa
      481 ctctgggtag taataaacag cattctgata gagagtgcct gtataaagct gaagtgacaa
      541 tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt ctttttcatt gtaaatatcc
      601 tggcattact aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt
      661 aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca
      721 gatctcatat ctctctcagc aatcctcaat aatcagatac accgctttca ccggaccatg
      781 aaccccgacg actttgataa gctcaatatc cgccgttgaa ctagggccgc taatgatgtt
      841 aatgcaggac ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg
      901 cgctacacgc ggcaagatag tgcttttacg caggataaaa agagaagatt ccgggagcag
      961 gctcaatgaa cgcccgcgcc cggcggcgga aaaaagaacc acgcctcccg attcggttaa
     1021 accatattca gcatacacga cacccacttt agcctgctcg gcctgcgaga tattctcggc
     1081 acctttcgcc ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cactaatccc
     1141 caattcctcc agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca
     1201 cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat
     1261 aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gttgggtaag
     1321 ccgctcgtta gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg
     1381 gcgacccagt gcctgagcaa cgttattcaa aaattcgcct cgattatcca ttctttttct
     1441 cctgcgcctg atgtttctta aaccaactac ggaaactctc tccgtcagct tcaggaagat
     1501 cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag tggtgttttg ccgccattga
     1561 taaaccagct tgccgcatga gcgccggcca tcatcccgac tttccacaat cctggatgac
     1621 tattggcata agcgaacatt tttatcgccc gttgctctgc ttttgcggtg ataccttttt
     1681 cagccatcac ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca
     1741 cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc
     1801 cgccaagtag cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat
     1861 gaccgccaat atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc
     1921 gcagcacatc ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca
     1981 gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt
     2041 tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta
     2101 cctcggcaaa cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca
     2161 gcgtggtaca cattcgcgca ttaccttcat tggtcaccag gcataccgaa cctgtctctg
     2221 ccaccgcgaa attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt
     2281 tttgccggat gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt
     2341 cgtgtagcac tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag
     2401 atggcggatc ttgatccagc tggagaatgt attcacccag atcggtttca atcacctgaa
     2461 tgccagcatc ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt
     2521 tcaccacctt ccgggcattt ttgcgttggg caacctgtaa aatgtagcga gtggcgtctt
     2581 ctttagtttt tgcaaaatag acgtgaccgc cgttttgcgt cactttttct gagagctggt
     2641 acagataagc gtcgagatta cttagaacat gatcacgtat ctgggcggcc cgatcgcgcc
     2701 actcctccca gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct
     2761 gcgcgtttgc caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct
     2821 taaaatctgt attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact
     2881 tcagcaatat gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc
     2941 agcaggcaac tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca
     3001 accttttctt tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca
     3061 aatccgcagc aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc
     3121 agcagcgtaa gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga
     3181 tacacagctc tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata
     3241 aaagaggtga gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcaggt
     3301 tcatccgcca gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa
     3361 ataatgggat cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct
     3421 tctttgatat aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa
     3481 tttacgcgac agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc
     3541 agggcgtcac caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt
     3601 catgcccatt tatgcccgat gtttcccccc caccggggga tcaatcatca ataaatatgg
     3661 cgttcttttt taattggcta gcaacatacc acccctcatt tgtatgacaa atgataatct
     3721 ttcatccaaa cttcaaatgc aataactaat tttatttctt tcggcatcgc tcacatttta
     3781 tggggaaatc gacgttcatt tttggcataa gaaatccatt ta
//
LOCUS       DAZFRJ010000292         3821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000292 DAZFRJ010000000
VERSION     DAZFRJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.294"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..65)
                     /locus_tag="ACHKUN_004594"
     CDS             complement(<1..65)
                     /locus_tag="ACHKUN_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662708.1"
                     /translation="MAISAGRLTQMISVLNPVLTR"
     gene            complement(76..402)
                     /locus_tag="ACHKUN_004595"
     CDS             complement(76..402)
                     /locus_tag="ACHKUN_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6662709.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(399..2821)
                     /locus_tag="ACHKUN_004596"
                     /pseudo
     CDS             complement(399..2821)
                     /locus_tag="ACHKUN_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(2767..2988)
                     /locus_tag="ACHKUN_004597"
     CDS             complement(2767..2988)
                     /locus_tag="ACHKUN_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662710.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(3033..>3821)
                     /locus_tag="ACHKUN_004598"
     CDS             complement(3033..>3821)
                     /locus_tag="ACHKUN_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6662711.1"
                     /translation="FNIRVHAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIA
                     VLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTA
                     STGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGG
                     SFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSP
                     VELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          785 a         1254 c          937 g          845 t
ORIGIN      
        1 ttacgggtta acaccgggtt cagaacactt atcatctgtg tcagacgacc tgcagatatt
       61 gccattcctc cctcctcata acaccgtcgg acaacgcaaa tcgtaaatca gcacggacac
      121 agaaaacggc agttccccct gcacgaggtc ttcccgctca gcaagatccg gattccggta
      181 cagcatcccg gtcagtcgca tggcagcccc cttcatccgg gttaatgcct cgcccgggat
      241 cagctcaccg tcctcacgaa tcactttatc ccggctgccc tgaatgtagg ccagcagcac
      301 ggcggtagcc tgacgaacct tgtccatcag catgtcatca tccgcgtcat ggtcaacacg
      361 cagatgtgcc ttgatctctt ccagtgtcag taatgccgtc attttccgcc tcctgcatcc
      421 cgtccacgtt ttgcagccag ggtccagcct gatgaatgag cttctccggg tttatcaccg
      481 gtcatactgt tgcagtgcca cagcgagccc ccccacgtca ccgtatcgcc ggggtggtag
      541 gtttcaccgg ctctgaacac accgcggtag agcatcaccg gcagggaaaa tgttttttcc
      601 gtacactggc cactgctctg ccggatcacc acagagaaca accgctcatc cgtcatgctg
      661 acgtcgatat ccgccacccc gtcaaccagg cattcccatc cccgcatccc gtgcgttttt
      721 tcatacgccc gccagagtcc acccaggtgt gtggcatacg tgccccgggg aaaggatttt
      781 tgatcgtcaa tagcggggag cacttccagt gccgtggcat cacgcccgtc ctgcggagcc
      841 ggaagggcat tcaccgcctc cagaaccgcc tgcttcagta cttccggatc gtaatcacga
      901 ccatcacgcg gagcagggat atggcttacg gcctctttca ccatctgctc aagcatcgga
      961 cgcacatcat cgggggtgat acttttgccg tccgccggta ccggaatatt cgcaaccgca
     1021 tcattcaccg cctgcttcag tacttccgga tcataatcac gaccgtcacg cggagcaggg
     1081 atatggctta cagcctcttt caccatctgc tcaagcatcg gacgcacatc atcgggggtg
     1141 atacttttgc cgtccgccgg taccggaata ttcgcaaccg catcattcac cgcctgctgc
     1201 agtacatccg gatcataatc acgaccatca cgcggtaccg gaatggtccc cacagcgtca
     1261 tccaccatcg cctgcagaac cggatgtacc tcatccaccg tcacatgctt ctgtaatacc
     1321 gccgacaggg aagccagttt ctcttcaaac gcttgtgcct gcgcggccat cttcccctca
     1381 aatgtgcgct gtaaatccgc cagcaccgtg gagaattctt cgcccagtgc acgaataatg
     1441 gacagttccc gttccgtcat tttctcagta tccccctgaa catcgctttc actgcatcat
     1501 gctctgtttc actgattgcc ttattaccgt cagatgcgcc gtcaggcagt tgtgctgaaa
     1561 ctgttttccc ggccgacgcg aacgggtcct cacgggcatc acgacgggac agcgcctcca
     1621 gactgtagtt ctgctgctga agatacagtg catcaccacc ggcaaggggc ggcaggttct
     1681 cacgtttacg ggcctcattg ggcgtgagaa gcgtattttt cactgattca cccagtgttt
     1741 tcatgcgccg ttcgctgtcc attctcagca gcgtggtgac gtcaaactcc gtgctctcgt
     1801 tttcccccgt ttccagcgcc tcatccagta acagctcaat ggactcaatc agcgtctgca
     1861 gacactggga ataatactgc tgctccagcg cctccacgtt gtcactggaa ggcgggtggc
     1921 caacgccaat cttgtaggcc gggacacgga acaccgaaca gacaatttca gccgtcattt
     1981 tcagttgttc caccgtctgc gcatccaccg gtgaaaacgt cgtggggctg tattttgccc
     2041 cgttgctcag tatggccgtc ttcccggcat tttcgcccgt atatccgctg tcccagttcc
     2101 ccttcagttt tttcgcgttt tcttccgtaa tactgccggg gacctcaatc acgcctgatg
     2161 gtcgcccgcc atttctgaaa aaatacgtcg aatttgcctg aatatgatgc ccctgcatgg
     2221 cggccagtcc tgcggcatac accggcggca gccccacaag cggatgaaaa aaacagttaa
     2281 accggtcgtg gatcacctcc cgggcaggca ccgtcaccga ctctgtgatc ccacagttcc
     2341 ggtccggcgt gatgcggtag aacacgtcgc cgtcatccgc caccagaggt tcaacccggt
     2401 tccagtccag aatacgcagt tctttgatct gcccccgggg gttgcggatt ttcagcacca
     2461 ccgtattgcc gtgacgcaat ttggaattca gccacagttc aaaaaactgg atgcgattct
     2521 gctgcgcatt aggacgacga cagagacggg cagtatctcc ctgccgcttt tcccggcgta
     2581 ttccctgtac atcagtctgc atcaggcgaa gccgcatttt ggcaatatcc tgggatatca
     2641 gcgaaatgca tgaaaacacc gcatgaaagg agaggacact ttccggatcg gctttcacac
     2701 cctgctgcca ggcaccggca aaaggctcag ccaccgcctg aaacagggac cgccagccca
     2761 cttctcttac gtcacgtcct gatttctggt tttttcgggt tcgccgcaaa aggttccaca
     2821 ttcgccatgc tccgcatcac gtttcttttt ctgacctgcc ggacgtcgca ctgtgatgta
     2881 ctccgccttc cccaggcgaa ccagcacctc cgcacacggc tgtgccacat cacggatatc
     2941 cccggcccgg gcatcatgcg tgccctgcag atatcggatc tttgccataa cctgttacgg
     3001 gaggcgcacg cctcccgtcc tcctcatcag actcagccgc cggacgcact gccgtagttc
     3061 actccggtga tcaccgccac cgccgcggta cggcgacgac gccagttgat ccagcgctcc
     3121 gcacggatgg ccacgctgcc tgtctggaac atggaaacca gctccaccgg ggacggcgtg
     3181 gtactgtcgc cggtcggctc agactgcatt tccagtgatg cctcgcggga catatccact
     3241 gccacgccgc cgtcatccgc cagataaata tccggggcat tcaccagcac cagctggtca
     3301 cccacgtact gggagacaat caccggcagc ccctggaagg agccacccag cagggtcatg
     3361 tccgggtatt ccttctgacc cagcgcattt ttacgcatgg acagtgccag ggcattggtg
     3421 ctggacatca gccagaccgc accggtgggc tgcaggtttg ctgccacaaa ctgtccaaac
     3481 gccgcctctg catccgcatc cgggttaccg gttgatgccg tgcccttcac atcatgggtg
     3541 atggacgccg gggagacatc tgccactgcg gcttttttcg ggtccacaaa gtctgtatcc
     3601 agacgcgcca ccaccgcttc cgccagcgca ttacggacca gtgcatcagc agccggactg
     3661 gaaaaacgga tcaattcttc cgtcagtacc gcaatggccg acactttcgc atgactgaag
     3721 gtgatggatt caaaatcaaa cttcgtcagg ggttttgcct taccctcacc cacccagccg
     3781 gcagcaccgc cggacacctg ggcgtgcaca cggatattga a
//
LOCUS       DAZFRJ010000293         3808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000293 DAZFRJ010000000
VERSION     DAZFRJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.295"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..1674
                     /gene="dpiB"
                     /locus_tag="ACHKUN_004599"
     CDS             16..1674
                     /gene="dpiB"
                     /locus_tag="ACHKUN_004599"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO6662712.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1643..2323
                     /gene="dpiA"
                     /locus_tag="ACHKUN_004600"
                     /gene_synonym="citB"
     CDS             1643..2323
                     /gene="dpiA"
                     /locus_tag="ACHKUN_004600"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO6662713.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2364..3749)
                     /gene="dcuC"
                     /locus_tag="ACHKUN_004601"
     CDS             complement(2364..3749)
                     /gene="dcuC"
                     /locus_tag="ACHKUN_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6662714.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1025 a          916 c          996 g          871 t
ORIGIN      
        1 cccgcaagga aaacaatgtt gcagcttaac gagaataaac agtttgcatt tttccaaaga
       61 ctggcatttc cgctgcgtat ctttttgctg attctggtgt tctcaatatt tgtcattgca
      121 gccctggcgc aatattttac ggccagtttt gaggactatt taacgcttca tgtacgcgac
      181 atggcaatga atcaggcgaa aattattgcc tccaatgaca gtgtcatctc tgcggtgaaa
      241 acgcgtgact acaaacggct ggcgaccatc gctaacaaat tacaaagaga taccgatttt
      301 gattatgtgg tgattgggga ccggcactcg atccgccttt accatcctaa tccggagaaa
      361 attggttatc ctatgcagtt caccaaacag ggcgcgctgg agaaagggga gagctacttc
      421 attaccggga aagggtcaat ggggatggcg atgcgcgcca aaacgccaat ctttgatgac
      481 gatggaaaag tcatcggcgt ggtgtcgatt ggctacctgg tgagtaaaat cgatagctgg
      541 cgggctgagt ttttattacc gatggcaggc gtgtttgtcg tgctgttagg gattctgatg
      601 ttgctgtcgt ggttcctggc agcgcatatc cgtcggcaaa tgatgggcat ggagccaaag
      661 caaatcgcac gcgtggtccg tcagcaagag gcgctgttta gttcggttta tgaagggctg
      721 attgcggtgg atccgcatgg ttacattacc gccatcaatc gtaacgcaag aaagatgctg
      781 gggctgagct ctcccggacg gcaatggttg ggtaaaccca ttgctgaagt ggtcaggccc
      841 gccgatttct ttaccgaaca gattgatgaa aaacgtcagg atgtggtggc taactttaac
      901 ggtctgagcg ttattgccaa tcgggaagct attcgttcag gtgatgattt gctgggggcc
      961 attatcagct ttcgtagtaa agacgaaatt tccaccctca atgcgcaact gacgcaaata
     1021 aaacaatacg tcgagagcct tcgtacattg cgacacgagc atctcaattg gatgtcgacg
     1081 ctcaatggtc tgttgcagat gaaagagtat gatcgcgtgc tggcgatggt gcagggggag
     1141 tctcaggccc agcaacagct tattgacagc ctgcgtgagg cgtttgccga tcgccaggtg
     1201 gcggggctgc tttttggtaa agtgcagcgc gcccgggaac tggggctaaa aatgatcatt
     1261 gtccccggta gccagctttc gcaactgccg ccaggactgg atagcaccga gtttgcagcc
     1321 attgtgggca atttacttga taacgccttc gaagccagcc tgcgtagcga tgaaggaaac
     1381 aagatcgttg aattattcct cagcgatgaa ggcgatgatg tggtgattga agtcgccgat
     1441 cagggctgcg gcgttccaga gtctctacga gacaaaatat ttgagcaggg ggtcagtacg
     1501 cgtgctgacg agcccggtga acatggcatt gggttgtact tgattgccag ctacgtaacg
     1561 cgctgcggtg gtgttatcac tctcgaagat aatgatccct gcggtacctt gttttcaatc
     1621 tatattccga aagtgaaacc taatgacagc tccattaacc ctattgatcg ttgaggacga
     1681 aacgccgctg gcagagatgc atgcggaata tattcgtcac attcccggat tcagtcagat
     1741 attactggcg ggaaacctgg cgcaggcccg aatgatgatc gagcgtttta agccggggct
     1801 aatcttgctc gataactatc ttcctgacgg cagagggatt aatttattgc atgaactggt
     1861 gcaggcgcat tatcccggcg acgtggtgtt taccactgca gccagcgata tggaaacggt
     1921 gtctgaagca gtacgttgtg gtgtatttga ttatctcatt aagcccattg cctatgaacg
     1981 gctggggcaa acgctaaccc gtttccgcca gcgtaaacat atgctggaaa gtattgatag
     2041 cgccagccag aagcaaattg atgagatgtt taatgcctat gctcgcggtg aacctaaaga
     2101 cgaactgccg accggcattg atcccttgac gctaaacgcg gtgcgaaaac tgtttaaaga
     2161 gcctggtgtg caacatacgg cagaaacggt ggcgcaggca ctgaccatca gtcgcaccac
     2221 tgccaggcgt tatcttgaat attgcgccag ccgccatctg attattgctg aaattgttca
     2281 cggcaaagtt ggcagaccgc aacgcatata ccacagtggg tgacatgata aagccggagg
     2341 aaacttgcct ccggcattac tatttacttg cctgtgaccg ctgctgccgt tcctggcacc
     2401 atcagctctg tagcaacgat aacaatcacc aaaccaacaa gcaccggtac cgaggtgcgt
     2461 tttacgactt caaacggcga gatcttcgcc atcccggcaa ccgcaacgac tacgccagaa
     2521 acgggcgaaa gggtacggcc caggtttgac gcctgcagca tcgggatagt caaatacgcc
     2581 gggttaatgc cggaagagtg cgccagtttc gggatcatct caacaaacgc ataaaacggc
     2641 gcattgcctg aaccggtcgt gactgccgcc agcatggtca gaatcaccaa taccagcatc
     2701 aggatgatac tcgccgaacc aaacgaggta gcgatagaaa tcagactttg aataaagccg
     2761 atggtgctaa gcccctgagc gaatacccca gcggcaacca gcagcatcac cacgttagca
     2821 aacgcatctg ccattccgcg ataagccact tccagaccag agaaaacttt ctgggtatta
     2881 aagctgcgga ggaactccag aatggaggca atcagcatac aaatcaccag aatagtgatg
     2941 atgtgtaatt gcggacccca tttaccgtca aaaatcagta caccgatgat cggcgtgaac
     3001 ggcaaaatgg cataaaacgc aggagcagtg gtggtgattt cactgacatc taacatttca
     3061 tgagagatgt gctctttttt atccagataa cgttgccaga agaagtgggc gatcgccatg
     3121 ccgataattg cagcaattga gataggcagc gtcgttttga aggcgaagtc aatcagcgac
     3181 atttcggaag cttgcgccgc cagcaccaca tcccctgaag tcggtgcgag aataatcgcc
     3241 gccggggagg cacaaatggc agcagctgcg ccacgactga taccaacgtt taccatcacc
     3301 ggaaataggg ttgccatcag caaaacaccc agaccggttg cggaagagac ggccagagac
     3361 atcagacagg cgacaaaata ggcggcaatc atcagcaggt aaggggagtt aatatactgc
     3421 aatggttttg acgccagctt gaccaccata tcattcgcgc cgatatgggt catgtaagcg
     3481 gcaaatccac acagcatcat aatcatcatg ccgaggtcgc cgccgcggct cattagtaat
     3541 attttaacgt attcaacgat atccgtggcg ctgtagcctg ttgaagcctg gctggacggt
     3601 aacactttgt gccccataat ggcactgata atcagcaata acaggccacc gacaaataac
     3661 acaccagtgg cggaataccc tttaatgatg tagcgagcta cacccacaat aaccacaacc
     3721 ccaataagga gttcaatgaa tgtcagcata atttttcctg tctccaggcc ccaaagtaaa
     3781 taataaaaaa ttcctaaagc ttaaggaa
//
LOCUS       DAZFRJ010000294         3742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000294 DAZFRJ010000000
VERSION     DAZFRJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.296"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..730
                     /locus_tag="ACHKUN_004602"
     CDS             2..730
                     /locus_tag="ACHKUN_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO6662715.1"
                     /translation="MLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHALSGNILL
                     LVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICP
                     VQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIAST
                     SQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDEDFSLKNS
                     RDGNPRKWRFDATNSELCAERHEC"
     gene            657..2779
                     /locus_tag="ACHKUN_004603"
                     /pseudo
     CDS             657..2779
                     /locus_tag="ACHKUN_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2949..>3742)
                     /locus_tag="ACHKUN_004604"
     CDS             complement(2949..>3742)
                     /locus_tag="ACHKUN_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO6662716.1"
                     /translation="MQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQ
                     LKAKTNFQVVGLTMKCYSPLCKNASRMDQNLRTPVPMTWAFMMREAGFPAVRSIYELP
                     LSDDVLDEVREEMRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYG
                     PKGEDKARALVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVV
                     AIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1090 a          864 c          853 g          935 t
ORIGIN      
        1 attgcttata actcattata tgtcaacatg ggtcactata acggaagcag ttgagatcac
       61 taccaaagcc ataaaacaaa aaattactcc tagcgatatt tatcgtcatg ccttgagtgg
      121 caacatccta ctattggtct atttccagtc tcctgtgata cttaagaaga tacaaacttt
      181 taatggaaaa ataaaattca ggcagtttgt gggagaccta cttgataaac tatgcatgct
      241 tgacagggac ggttttattt atggtcaaaa tttaagactc tgtacagaag ccagatatat
      301 ttgtcctgtg caacaaatta tagatacccc cctgttacgc aaactcaacc agtttcgtac
      361 gtttgttcgt aatgtccgcc cgggtgatga actggatgtc caggcacaag ttagtgaaaa
      421 aaatttaacc ccgccgccgg gtaacagcag tggcaacctt gagcaacaga tagccagtac
      481 ttcacagcta attggatctc tgctcgccga agatatgaac agcgagcaag cggcaaacat
      541 agcacgtgga tgggcctctt ctcagacttc aggggtaatg acagactggt taagccgctt
      601 cggtaccgca agaatcacgc tgggcgtgga tgaagatttc agcctgaaga actcccgtga
      661 cggcaaccct cgaaaatggc gattcgatgc aacaaacagt gaactatgtg ccgaacgtca
      721 cgaatgctga aatcacgctg gcagccttga aggatccggt gattgccgac aataacgatc
      781 tcacgacact aacagcaccg tcgctgatac agagggcaat gcgatagcca acactgaggt
      841 aacatttact ctaccggaag atgtgaaggc gaacttcacg ctgagcgatg gcgacaaagc
      901 ggttactgat gccgatggca aagcgaaagt cacgctgaaa ggtacaaaag caggcgctca
      961 taccgtgaca gcatcgatgg ttggcggtaa gagtgagcag ttagtggtga actttactgc
     1021 ggatacgctc actgcgcagg ttaatcttaa cgttaccgag gacaatttca tcgccaataa
     1081 cattggaatg accaggctac aagcaatagt gactgatgga aacggcaacc cagtagaagg
     1141 tataaaagtt aatttccgtg gaacctccgt cacgctaagc agcaccagcg ttgaaacgga
     1201 tgatcaggtt tttgctgaaa ttcttgtgac aagcaccgag gtcggactaa aaacagtttc
     1261 agcctctctg gcagataaac ctactgaagt catctcgcga ttactgaatg ccaaggtaga
     1321 tgttaattct gcgacgatta ccagtcagga gatacctgaa ggtcaggtca tggtcgcaca
     1381 agacatagca gttaaagctc acgttaacga ccagtttggc aacccggtga cgcatcaagc
     1441 cgcgacattc agtgcagcgc catcctcgca aatgatcatc agccagaaca cggtctctac
     1501 taatacgcag ggagtagccg aggtcaccat gacgtctgaa agaaacggct cgtatactgt
     1561 aaaagcatcc ctagcgaatg gagcttcact tgagaaacaa ctggaggcta ttgatgaaaa
     1621 actgacactc acgtccagtc cgcttatcgg tgtcaatgct cctaaaggcg ctactctgac
     1681 ggcaacgcta acctctgcaa atggcactcc agtggagggt caggtcatca actttagcgt
     1741 aacgctagaa ggtgcgacgt taagtggcgg aaaagtgaga accaactctt caggtcaggc
     1801 tccagtcgtt ctgaccagca ataaagtcgg tacatatacg gtgactgcat cgttccataa
     1861 cggcgtcaca atacagacac agacaaccgt gaaggttact ggcaacccaa gcaccgcaca
     1921 tgttgctagc tttatcgctg atccgtcgac tatcgccgcc accaacagtg atttaagtac
     1981 cttaaaggca acggttgagg atggcagtgg taacctgatc gaaggtctca ctgtgtactt
     2041 cgccttaaaa agcggctcca ccacattaac gtcattaaca gcggtgaccg atcaaaacgg
     2101 aatcgcgaca acaaacgtga gatgagcgat aacagggagc gtcactgtaa gcgcagtcat
     2161 aagcgcaggt ggaatgcaaa cagtagatat atcgctagtg gcaggcccag cagacgcctc
     2221 gcagtccatc cttaagaaca accagtcatc actgaaaggg gactttaccg atagtgctga
     2281 gctacatctt gttctgcacg atatatcagg caatccgatc aaagtttctg aagggatgga
     2341 atttgtgcaa tcaggtacaa acgtgcccta tatgaaaatt agcgcaattg attacagtca
     2401 aaatatcaat ggtgattaca aagctactgt tacaggagac ggagagggta tcgcaacgct
     2461 gatccccgta ttgaatggtg ttcatcaggc gggcctgagt accacgatag aattcattag
     2521 cgcggaaacc agaccaatga cgggtacagt gtcagtgaat agtgcaaacc taccgacagc
     2581 ttcattccct tcgcagggat ttaccggggc gtattatcag ttgaataatg acaactttgc
     2641 accaggaaaa acggcggctg attattcgtt ttcaagctcg gcctcctggg taggcgttga
     2701 tgctactggt aaagtgacat tcaaaaatga cggtgatagc aatacagtaa taatcacagc
     2761 gcccccaaga tccggtggtg ctatttacca aacagttcct cctgaatcgc gttcggtgtg
     2821 actatgatca ttttcggtga gtattgcttt gatccaaagg ctggttttct gtgatgattg
     2881 accagcctca gagtattgat gacaccaccg ttcataacag atcggcatta ctatgcataa
     2941 gtactgcctc aaattctcgc caaaacactt tttaatgcct ttgcgaattc atccagactt
     3001 atattgtcaa ttttttgccc aacgacgact gactccgaaa catcggccat tgcgggccat
     3061 cgcgttttgt aatcagcata aattgcaaca acaggggtat tataggcact tgccatatgt
     3121 aatattgagg tgtcaggagt tattgcaata taagcatctt ttatgattgc tgctgaacgt
     3181 ttaatcgagg gtgataagga taaacggtag acattattat aacaatccac taatgccctg
     3241 gctttatctt cccctttggg tccataaacg atcactattg gaatatctgt ttcactttga
     3301 atttttgcta ttagattttc tgcaatcgat aatgaaaatg tacgttcctg cgagctaccg
     3361 tctaaattga gcgcaatgta agatcctaac gatcgcattt cctcgcgtac ctcatcgagt
     3421 acatcatcgc ttaatggcaa ttcatatatt gacctgactg ccggaaaacc cgcctcacgc
     3481 atcataaatg cccatgtcat gggtacagga gtccggagat tctgatccat ccgggacgcg
     3541 ttcttacaca aaggggaata gcacttcatg gttaaaccga cgacctggaa attcgttttt
     3601 gctttcaact gactgataaa tatcatcgtt ttcaggttct ttttacgcat cgcctctata
     3661 caaagatccg gcgtaccgta ttgcagtgtt atattcttcg ctaaatcttt tatttctttt
     3721 aatgttgcgt gatcctgcat ag
//
LOCUS       DAZFRJ010000295         3718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000295 DAZFRJ010000000
VERSION     DAZFRJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.297"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            4..600
                     /locus_tag="ACHKUN_004605"
     CDS             4..600
                     /locus_tag="ACHKUN_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662717.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            1062..1562
                     /locus_tag="ACHKUN_004606"
     CDS             1062..1562
                     /locus_tag="ACHKUN_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO6662718.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(1778..2004)
                     /locus_tag="ACHKUN_004607"
                     /pseudo
     CDS             complement(1778..2004)
                     /locus_tag="ACHKUN_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2294..2728
                     /locus_tag="ACHKUN_004608"
     CDS             2294..2728
                     /locus_tag="ACHKUN_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO6662719.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKSLALALRRCPK"
     gene            2821..3087
                     /locus_tag="ACHKUN_004609"
     CDS             2821..3087
                     /locus_tag="ACHKUN_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662720.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSERKRVRIPAMKVSEWGHLLRALELERVCH"
     gene            3265..3654
                     /locus_tag="ACHKUN_004610"
     CDS             3265..3654
                     /locus_tag="ACHKUN_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO6662721.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVSTLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
BASE COUNT          740 a         1014 c         1121 g          843 t
ORIGIN      
        1 aatatgatga aatcagacga aaaataccag gttcccgcgt ggatgcgacc tctgttgccg
       61 ttgctctgca acaccggggg gaacgatccg gaagaactgc tgaatgatac agaaaccact
      121 gccagtgcga atgttgtccg ttatgtactg atagttgctg tgcggtcgca ggttgatctg
      181 ctgcagcttc tgtacaggaa agggctgttg cgcacagaga taccaggtgg cttttcaccg
      241 gaagaagcgc aggcactcct ggataatctg gtgcgcagcc atatcagcaa ggcgctgtcg
      301 ggcgagcgaa tggcagcccg tgacagaaat gccgatctgg cctggattgg acagcaactg
      361 gtcgatgccg cctggtttgt ccgtgccaca ctggaagcgc atgggatgag cgtcggaaat
      421 gagagtcccc ctgctccgcc ggagacaatg ccggacatac agacacggga actggtgatg
      481 ttgatcaagc gactggcatc atcgctgaaa gcggtgaaac cggacagttg tgtggtgcgt
      541 gaagcgcagg actggctgcg cgacagaaaa cttgtggata tcacagatat tctccggtga
      601 accaggcatg tatattttac gcagggacat gccggtcatg atcgtttcgt tccaggggtg
      661 gtctggagat catgcggtgt gctccctgct ctcgccggaa taaggacgtc gccgccggct
      721 ccgcttttac ccggccatgc ggggcatggc cttgtgggtt ttcagttatg tggccgccgt
      781 gtcgtgtgcg ggctgtgccg tgcctccatc gtagccgggc ttctgcgggc gggatgcaag
      841 gtaagatgaa ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc
      901 gttcacccgc tcagatttcg cggtctcacc ctccaactcc cgtcagccgt gcgcgtcggg
      961 ctgcggcttt ccttgcgccc ttgcatccct gcaggcccgg caaagatgga ggcacggcac
     1021 cgcccggtgt cgcagaacat gtgactatgg aggattcggg aatgtctgtt gttgcacctg
     1081 ctgtatacgt tggaacctgg cacaaataca actgtggaag catcgccgga cgctggtttg
     1141 acctgaccac gtttgatgat gagcgcgact ttttcgccgc ctgccgtgct cttcaccagg
     1201 atgaagccga tcctgaactg atgtttcagg attatgaggg attcccgggg aatatggcct
     1261 ctgaatgtca tatcaactgg gcctgggttg aaggcttccg ccgggcacgg gatgaaggct
     1321 gcgaagaggc ttatcgtctc tgggtggatg ataccggtga gacggatttt gacagcttcc
     1381 gcgatgcctg gtggggcgag gctgacagtg aggaggcttt tgcggttgag ttcgccagtg
     1441 ataccggcct gctggctgac gtgccggaga cggtggcgct ctattttgac tatgaggcgt
     1501 atgcgcggga tttattcctg gactccttca cctttattga cggtcatgtg ttccgtcggt
     1561 gatttactcc cccgctccgg cgggggtatg gcctgccagg ccgccggaaa accctggcag
     1621 gcttacgcct gcctgaaatg caggagggcc gcgcctgcgg cgctgccctt cctgcccgtc
     1681 atggtgtgag gcatttttct gttatgtcgt ttctgttgtt ctgatttccg gaggcgggga
     1741 caccttcggc tcccggctgc ctggcggcat ccggtcgtca ggcccgactc cgacaggcag
     1801 ctgctggcgc acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgccgctac
     1861 ggtccgatgt ctgaaaaacc aggcttgcag cgaccgttcc ggtcgcgccg cctctgtcgc
     1921 gccgtgacct gcgctccctg cacacctgac ggtgtgtaag gaaactgcgc ctgatggcgc
     1981 agttgtctgc ccttccgccg ccacccctgc ggcctgtttc ccgctgtgga ctttccgggg
     2041 gctgaagcgg ccacaacacg tgtcagccgt ccagggggga gatgtaccag ccacaaaact
     2101 ttttgccgca agcgtcaaaa acttttgcgt ctgcccccgg acaactgccc cgtgccgtgg
     2161 ctgtcgctgc cccatcggaa aggtccactg cggggagaaa ccccgaagaa gtggacggca
     2221 gaaggggctt tcgcccctcc gggggagcaa ccggtcggcc cggcgacctg attcagcaga
     2281 aggagacgtt ttcatgacag tcagcagcac gatttccgtt ttttgccgcg acggggtgtt
     2341 ccgtaccgtt tactgccacc tgcacggtga gccgacctgg aacggtcgca tcctgcatac
     2401 ccactatgcc accggtcagc tggccgaagc cctggttgaa cacggagata tccgttgcct
     2461 cggtccccgt tgcgacaaac ccgccgggca tacgcttcag aacccggtgg acggtgtgac
     2521 ggcttattac ggacgtgaca gtggtttccg gatggacagt gaagcgcgtg agtaccgctc
     2581 tttcagggag gctattgcca ctgaaagcac agaagaggtg cgcttccatt atgtgttcat
     2641 cgacggctac tggaaggtga tgtaccgcac gccggaaggc tggaagatga agtcgctcgc
     2701 gctggcactg cgccgctgtc cgaaatgaaa aaaggcaggg cgataaaccc tgcctctctc
     2761 cgccgttact tcccagcccg gagtaccggc atctcaatca ctatctatgg agattatgct
     2821 atgagaccat caattatctt cgcaaccgcc gagtatgtaa agcgtctgcg tgaagagtgt
     2881 ctgcgggaga ataaacccct gcaccgccat acccgcttca gacgtcagga gctggcacag
     2941 gatgagatta acccggacgt cctggcgatg agcggacata ttgccagacg ctgcagtgag
     3001 cggaagcggg tgcgtatccc cgccatgaaa gtcagcgagt gggggcacct gctccgcgcg
     3061 cttgaactgg agcgggtctg tcactgatta cccccctgcc gtctgcctgt gcaggtggca
     3121 ggatgtgttc aggtacttta ccaggaggaa ccgtgagcaa gatgaaaaac atcacgacgt
     3181 ctgtgttgca tggtgtctcc acaaaaaaca gaaaagggaa acagattctc tttcattcag
     3241 ggcccgggag ggctggagat aacaatgaag tcttgttttg atactgacaa agcttttcat
     3301 atgtcttttg atgaagtcac tttcaatgct gacagcgttc gctcaaaact ggaactatta
     3361 tccgaagaac taatgcaccg ttgtggtctt gtttatgagc tatatcagcg acgtttgagt
     3421 gctgctattg ataattttgt gtctacattg ccgacagaac aacatctgct ggtcctggaa
     3481 ctggcccgtg aggagtttga ttacctttcc gctgaagaga ttgcagaaga aatacgccgt
     3541 gacgcagaac gtggctattg ttgtcacggc tttgatcgca attgctgtcc tcttggttgt
     3601 ggggatttgg acgatgattg ctgcgatgct gctgatttga tggaggaacc gtgagtaaga
     3661 agaaaaacac cacaacgccc acgccgcatg atgccgcgtt ccggtcgttc ctggcgaa
//
LOCUS       DAZFRJ010000296         3629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000296 DAZFRJ010000000
VERSION     DAZFRJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.298"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(92..874)
                     /locus_tag="ACHKUN_004611"
     CDS             complement(92..874)
                     /locus_tag="ACHKUN_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6662722.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(876..1289)
                     /locus_tag="ACHKUN_004612"
     CDS             complement(876..1289)
                     /locus_tag="ACHKUN_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662723.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            1849..2079
                     /gene="vapB"
                     /locus_tag="ACHKUN_004613"
     CDS             1849..2079
                     /gene="vapB"
                     /locus_tag="ACHKUN_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO6662724.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2076..2492
                     /locus_tag="ACHKUN_004614"
     CDS             2076..2492
                     /locus_tag="ACHKUN_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO6662725.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(2654..3342)
                     /locus_tag="ACHKUN_004615"
                     /pseudo
     CDS             complement(2654..3342)
                     /locus_tag="ACHKUN_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          891 a          941 c          948 g          849 t
ORIGIN      
        1 tggctttaac aattatgaaa taatacaaga gatcacatat tgcagtggtc tcttttttat
       61 agaagaaatc acaattcata attgtgactt attatgcatg tttgtttttc agcattgtga
      121 ccgcatcgga ctcaggcatc gaaaactgca cccggtgccg ggcagcgaca tccagcgcaa
      181 acaccttcgt gtagacctcc gttgagctga tggacttatg ccccatcaga ctctgcagga
      241 ccttcagcgg tataccggca tacagcatat gcatcgcata ggaatggcgg aacgtatgtg
      301 gcgtgaccgg gacagagaac gtcacaccat cagcggcagc ggcggcaacc gcctccccaa
      361 tccaggtcct gaccgttctg tccgtcactt cccagatccg cgctttctct gtcctgcctg
      421 ttcgtttatt acgccgttcc atggggattt tcaatgtggc caccatcgtc tgcagctggc
      481 tgacgtacca ggagtcggag agcggaacca gccggtgagt ctgctgcccg gcgggcgttc
      541 ttcctgccgt cctggcggct ttttcggtcc gttgtttcag ggtcgcaagc tgcacaaacg
      601 gatacggagg cgtgagcgaa aaatcccccc gcgtcagcgc cagtgcttca ttaatgcgcg
      661 caccggtgtt ccacagtgtg gccagcagca tcttgcggcg cagatccggg acgtaatgga
      721 gcagggcgct cacttcaggg gccagcaggt atttgggcag ttcatcatga accatcgaca
      781 tctggcggag tgccagcgct gccggataat cgatggcaac aggaagctga gcggcatccc
      841 gggcatggtg gatcggtgcc ggcaggtgct gcatatcagt gttcactcca cggggaaacg
      901 tcgggcaggt tttccacaac ccggtacttt cccggataac ggagctggtt accgccacgc
      961 gtccagacgg tgacaacaaa accccctccc tggttgtttt cggggagcag gcgatcgcac
     1021 tgctcactca gcatgtggat cacgaacggc gcatcgctgc cgtcttcaaa tatcagttcc
     1081 agcgcatcgc ggccatgcag cgttccctta ctgatgatga cgtactcagc cccccgcatc
     1141 tccctgagta aatgttttgc cgcatccggg acaagcagcc tggctgcgcc ggcattccag
     1201 ctgaggtaga catatccggc ctgcgcctgt acagactgcc agtaatccgt tctggcgaca
     1261 gcctgacctt cattctcaat aaaaaacatg ggtgactcct tttttctttt ccgggcaggg
     1321 acggtcatgc gataacgtca tccatcatca ctgctcttac cgtcacgcca tgtgataaca
     1381 tcatgccgtt atcatcatca ccatgataac aagcatccat ataaccacgc tgttaaatta
     1441 ccggaggacc atttccgaca gatccgattt ttctgttatt gacgggtttt cggaagtgta
     1501 tcacgctgag gaaataatca tatcgctgtt ttccgcccct gcgcgacgca tcacaggtca
     1561 gggagatgac acaacatata gtgtcatcag cagatgaatc cggcaccaca tgttgtgatg
     1621 acgggaggtg gcgttgtgtg gggggagagg aagatcctga acaacctttt ttcggatctc
     1681 cgataaaaaa gaaagaaata gagatatgct aaacatatgc ctggcatcta catatgctac
     1741 cgctatacat atgccacaca tctgcctata ctaaacatat gcacacaccc ggtgctttga
     1801 atattccgga tatgtttcac atatccatat gctcgggaga gaaagaacat gagaaccgta
     1861 tcgatattta aaaatggcaa caaccgcgcc atccgtctgc ctcgtgatct ggattttgag
     1921 ggggtgagcg agctcgagat cgtccgggaa ggggacagca tcatcctgcg ccccgtccgg
     1981 ccaacctggg gatcattcct agagtacgaa aaagccgatc cagattttat ggcggagcgc
     2041 gaggatgttg tcagcgacga agggcgattt aacctgtgaa caagacttat atgcttgata
     2101 cctgtatctg ctcgttcatc atgcgtgaac agccagaagc cgtcctgaag cgtctggagc
     2161 aggcggtgct acgcggccac cgcatagtgg tctcggccat cacctactct gaaatgcgct
     2221 tcggggctac cggcccaaag gcctcaccac gtcacgtcca actggtcgat gaattctgcg
     2281 cccgcctcga tgccatcctg ccctgggatc gcgccgcagt agacgccacc acgaagatta
     2341 aagtggcact gcggctggcc gggacgccga tcggcccgaa cgacacggcg attgccgggc
     2401 acgccatcgc cgccggggcg atactggtaa cgaataatac aagagaattt gagcgggtgc
     2461 ctgacctggt gctggaagac tgggtgaagt aagacactct tttcaaagtc aggagataag
     2521 ggcaccaaga aacagcccgt atacgggctg ttttaacaga tatctacagt aagcggtcct
     2581 aaactgtagg gtatcttcga agatcagact atcagggagc taaagcctat cttccggcgg
     2641 caaaaacagt ggctcaagcg atacgggcaa ccgagctctg gtttgctgcc ggtggcaggg
     2701 cgatgagctt gtcacattcc ataatcacgt actccgggat gagatcgaat ctggacggat
     2761 ctagttgaca gatgaactca ttttcaatgt ggtacgtttc gttaacgaat tttcgtatta
     2821 ccggatggtc ctgatccagt tccagtaaac ggaatacctg cagtttttca tagccgttac
     2881 gacagcgacg gtacaggttt tttatttcat ctgggctgct gacgagagcc tgaagtcgcg
     2941 ggtaatcaaa actgtccaca tactcccgga tatcccgcaa ggcctgctga attttctcag
     3001 gagccatttt ggggtagccc gagcccgcag cggcaggctc gcgataatcc agcggggcga
     3061 cgcgtcgatg gaatagattg gatagaagct gataagcatc accacgttcg tcaacgattt
     3121 caaagtagcg acgaagatag atgagcttaa tgatctcctc acagtctgct gacgcagtga
     3181 tggatttgca gatcagaatg tcatgatatc gccgtcgtta acaggtaatt cttctatgac
     3241 tccagcagac aggcgcaggc aggaggcggt cacctgatta ctgaacaacc ttctgactga
     3301 cttcagcgtg tcaatcacag gctccacgtc atgcgtcagc atagtgctga tgctgacgga
     3361 tgttacaaaa aatgcagcgt atggcgttga gatagaaagt ctggtgctgg agataaatac
     3421 acctacatca tgataaaaaa gggccagcga ctgacctgaa gaagacactg gctaaaaggc
     3481 tttgcatgtt tgtagatact tatttttcac agacagcaat gatgcctgtc aatatattat
     3541 taatatgctg attgtttcag ttacagatgc tttattaagg gaaaatgtca cgatcggtgc
     3601 aaaagtggtt tgaaccgccc cggttttcc
//
LOCUS       DAZFRJ010000297         3607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000297 DAZFRJ010000000
VERSION     DAZFRJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.299"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            424..990
                     /locus_tag="ACHKUN_004616"
     CDS             424..990
                     /locus_tag="ACHKUN_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6662726.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            965..1567
                     /locus_tag="ACHKUN_004617"
     CDS             965..1567
                     /locus_tag="ACHKUN_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO6662727.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            1564..2229
                     /locus_tag="ACHKUN_004618"
     CDS             1564..2229
                     /locus_tag="ACHKUN_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO6662728.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            2226..2849
                     /locus_tag="ACHKUN_004619"
     CDS             2226..2849
                     /locus_tag="ACHKUN_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6662729.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            3102..>3607
                     /locus_tag="ACHKUN_004620"
     CDS             3102..>3607
                     /locus_tag="ACHKUN_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662730.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIV"
BASE COUNT         1061 a          685 c          870 g          991 t
ORIGIN      
        1 gtctaactat agttatcacc aaaaacttgc ctcgatttta gatttttcca gtatttgtag
       61 atattgcact gaacaccgaa tacgtagcag agggtgtcta cacgataacg ttctatgagc
      121 taccatgttg tcgaaaaatt gtttagtgag tatgacatca ggaatgtggt ggtctgtttt
      181 aatatttcta tttctatttc tatttctatt tctatttcta tttctgtttg ttgtggtttt
      241 ttctttagtt catgtatttc gatttgtttc tgaattatcg ggggtagtgt cttccctttt
      301 ccctgaggct tatcatgcag ttattttcgc catcttggta ttgtggaaat accgacatcc
      361 atagctttgg tgagctctgt tcaggtggcc aaattcagaa aaccattacg gaggaagaag
      421 gcgatggcta aaccagcgcg aagacgatgt aaccgtaaaa gagaagattt aactgttaaa
      481 aggatatttg agttactaag tttcgataaa tctaccgggg tatttagatg gaaagttccc
      541 actcagggaa ggatagcatt aaatagtgtt gctggaactt ttgattccaa cggttattca
      601 atgatcatga tagatgggcg tagatataaa actcacgtct tagtttttta cataactcat
      661 aatcgttggc ctgctggtca aattgaccac gttaatggaa ttaggaccga caataggcca
      721 gaaaatttaa gagaatgcct gccaatagaa aattcaagaa atataaggat ccgaaagaat
      781 agcaaatcag gttgcagagg ggttacttgg cacaaacgac agaaaaaatg gaatgttagg
      841 ctaggattcc atggcaagag taaacacttc ggatgctttg atgatctgga gttagcggta
      901 ctagttgctg aagaagcccg agataagtat tacggtgatt tttccggcaa cgaaaggagc
      961 acttatgcga atctatcgaa ggaaatgtaa atgttgcaat gaatggttta taccaaaata
     1021 tcaaaatcaa tattggtgta atgagatttg tggaaccaag atagcactcg aacgacgaag
     1081 taaagaacgc gaaaaagcgg aaaaagcagc agagaagaaa ctacgacgag aggagcagaa
     1141 acagaaagat aaactgaaga ttcgaaaact cgccttaaag ccccgcagtt actggattaa
     1201 acaagcccaa caagccgtaa acgccttcat cagagaaaga gaccgcgact taccatgtat
     1261 ctcgtgcgga acgctcacgt ctgctcagtg ggatgccgga cattaccgga caactgctgc
     1321 ggcacctcaa ctccgatttg atgaacgcaa tattcacaag caatgcgtgg tgtgcaacca
     1381 gcacaaaagc ggaaatctcg ttccgtatcg cgtcgaactg attagccgca tcgggcagga
     1441 agcagtagag gaaatcgaat caaaccataa ccgctatcgc tggactgtcg aagagtgcag
     1501 ggccatcaag gcggagtatc aacagaaact taaaaaactg cgaaacagca gaagtgaggt
     1561 tgcatgaata tctacgaaag aattgatggc agcaaatacc gaaatatttg ggtagttggc
     1621 gatctgcacg gatgctacac gaacctgatg aaaaaactgg agacgatagg attcgacacc
     1681 aaaaaagacc tgcttatctc ggtgggcgat ttggttgatc gcggtacaga gaacgtagaa
     1741 tgcctggaat taatcacatt cccctggttc agagctgtac gtggaaacca tgagcaaatg
     1801 atgattgatg gcttatcaga gcgtggaaac gtcaatcact ggctgcttaa tggcggtggc
     1861 tggttcttta atctcgatta cgacaaagaa attctggcta aagctcttgc ccataaagca
     1921 gatgaacttc cgttaatcat cgaactggtg agtaaaggaa aaaaatatgt catctgccac
     1981 gccgattatc cttgtgataa atacgagttt ggaaagccag ttgatcatca gcaggtaatc
     2041 tggaaccgcg aacgaatcag caactcacaa gacgggatcg tgaaagaaat caaaggcgcg
     2101 gacacgttca tctttggtca tacgccagca gtgaaaccac tcaaatttgc caaccagatg
     2161 tatatcgata ctggcgcagt gttctgcgga aacctcacat tgattcaggt acagggagaa
     2221 ggcgcatgag actcgaaagc gtagctaaat ttcattcgcc aaaaagcccg atgatgagcg
     2281 attcaccacg ggctacggct tctgactctc tttctggtac tgatgtgatg gctgctatgg
     2341 ggatggcgca atcacaagcc ggattcggaa tggctgcatt ctgcggtaag catgaactca
     2401 gccagaacga caaacaaaag gctatcaact atctgatgca atttgcacac aaggtatcgg
     2461 ggaaataccg tggtgtggca aagctcgaag gaaatactaa ggcaaaggta ctgcaagtgc
     2521 tcgcaacatt cgcttatgcg gattattgcc gtagtgccgc tacgccgggc gcaagatgca
     2581 gagattgcca cggtacaggc cgtgcggttg atatagccaa aacagagcag tgggggatag
     2641 ttgctgagaa agagtgcgga agatgtaaag gcgtcggtta ttcaagaatg ccagcaagcg
     2701 ccgcatatcg cgctgtgacg atgctaatcc caaaccttac ccaacccacc tggtcacgca
     2761 ctgttaagcc gctgtatgac gctctggttg tgcaatgcca caaggaagag tcaatcgcag
     2821 acaacatttt gaatgcgatc acacgttagc gccatgattg ccacggatgg caacatatta
     2881 acggcataat attgactttt tgaataactt tggggaaact tgacaccaat aatgggcgtt
     2941 ttttacatgt cattgatgag tctcaataac ctgccgccga gtagttttta tgctctgaat
     3001 tgtatttgtg tagtaaacat gctgactgca atgtaataga gtttttttag cctgtaacct
     3061 cttgacggca ttgaattgct tttgttatga gttgtaagcc aatgttatca tcttgtattg
     3121 gggtggttat gaaggatggt gcgctgctca ggagttcttc actttttatt gcctacatgg
     3181 gatgccttgg atgggggagt gcttatttct atggatgggg tacttctttt tactacggct
     3241 tcccatggtg gattgtaggt gcaggtgttg atgatgttgc cagaagttta ttttttgcag
     3301 ttatcgtcat tgctatattt cttatcggtt ggggtattgg tgttgtattc tttttcgcag
     3361 tgaaaagaaa acattctatg caagagctaa atgtatttcg cctttatttt gctgtggaat
     3421 tattgtttgt gccggcaatt attgagtttt ctatattgag acagaagatt caggtacctc
     3481 ttttgctact gtcagcagcg attgcgctgg cggttacaat ttcgataaga tcttatgggc
     3541 gatttttatc ggtatcatgc ttctatgata agccatttat aaaaaaacat ttttttgaga
     3601 ttgtgat
//
LOCUS       DAZFRJ010000298         3597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000298 DAZFRJ010000000
VERSION     DAZFRJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.300"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..1009
                     /locus_tag="ACHKUN_004621"
     CDS             155..1009
                     /locus_tag="ACHKUN_004621"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO6662731.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1045..1935
                     /locus_tag="ACHKUN_004622"
     CDS             1045..1935
                     /locus_tag="ACHKUN_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6662732.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            1996..3495
                     /locus_tag="ACHKUN_004623"
     CDS             1996..3495
                     /locus_tag="ACHKUN_004623"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6662733.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            3496..>3597
                     /locus_tag="ACHKUN_004624"
     CDS             3496..>3597
                     /locus_tag="ACHKUN_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6662734.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGM"
BASE COUNT         1020 a          724 c          885 g          968 t
ORIGIN      
        1 aaacaaatta agttattttt tgtaagttat tttgatgata ttcgacatgc atcacgcttt
       61 ttgccgcttt tgctgtgcta gtttgctttc aaaatgaaag tgaatattct ctttcttcat
      121 tttgtaaggc gaacgacaaa taacaggagc tgcgatgtac atcatttcaa gcaaaaacat
      181 gctgaaaaaa gcccagtcag agggatatgc cgtccccgct ttcaacattc ataaccttga
      241 aactttacag gtagttgttg agagcgctgc acaacttcgc tctcccgtta tgctggcggg
      301 aacacccggt acttatcgtt atggtggcgt gggaagttta atttcgttag tacaatcgct
      361 ggctcgcgag tacaacctgc cgttagtcct gcatctcgac catcatgaag agagtgatga
      421 catttttaac aaggttcgcg cagggatccg ttctgtcatg attgatggct cccatttgcc
      481 atttgaagac aacattgcgc gagtgcaaga agttaccacc ttttgccatc gctttgatgt
      541 tagcgttgag gccgagctag ggcgtcttgg cggccaggag gacgatctgc tggtggatga
      601 aaaagattcc gcttatacct caccagccgc agctgcagag tttgttcaac gtacccaaat
      661 tgactccctg gccgtagcaa tcggcacagc acatggttta tatgcgcatg aaccacgact
      721 ggattttgtg cggctggaac agattcgtca gcgagtggag attccactgg tgttgcatgg
      781 tgcttcgggc ttatcagaac aggacgttca gcactgtatt cgccgcggta tttgtaaagt
      841 gaatgtcgcg acagaattga aaattgcgtt tgccgatgcg ttaaaaggat atttctacaa
      901 caatcctgcg gcaaatgatc cacgccacta tatgcagccc gcaaaggccg ccatgaaaga
      961 agtcgtaact cgtattattg gtgtctgtgg tagtgaagga aaaatttaag acgttgtacc
     1021 ctacaaaaac aatgaggatt gtttatgaaa aatattaaat tacttggttc agctatgttg
     1081 gtgagcctgg cattgttctc gcaaaattcg ttggcaaaag agtataaaat tggtgcgtca
     1141 ttattaacac agcaacatcc tttctatatt gatcttgcga atgccatgaa agaagaggca
     1201 aaaaaagaca atgtcaattt aagtgtctct attgccaatc aggatttaaa caaacaactt
     1261 tctgatgttg aggattttat tactaaaaaa gtggatgcca ttattatctc tccggtcgat
     1321 tcgaaaggcg tccaggcggc gataattaaa gccgataagg ccggtattcc cgttatcacc
     1381 gtcgacgtcg ctgcggaagg cgttccggtc gtatcgcatg ttgcgaccga taactatgcg
     1441 ggaggcgttg aagcaggcaa gttaatgggc aaacttttga atggtaaagg tactgtagcc
     1501 attatttctt atccggcact acaatcggtg gttgcgcgcg ttgatggttt taagaaaggg
     1561 ttaagcgata caccggatat taaaattgtt gctgaacagc ctggtattac gcgggctgaa
     1621 gcgttaacga ctgcgcaaaa cattatgcag gcaaatgcca acctgaatgg tctgtttggc
     1681 tttggtgatg atgcggcgct ggcagcggtt atcgcagcaa aatctgcgca taacgacaac
     1741 atcaaaatca ttggttttga cggcatgaaa gaagcgcgtg atgccgttga cagtgaaaaa
     1801 acgtttgcgg ccgtgattcg gcaatatccc gatcaaatgg gtgctaaagc cattgatgcc
     1861 gctgttgatc acctcaatgg taaaccggta gaaaaaatga tcccggtaca acccggcgtt
     1921 tatacaggta aatagtaaac atcttcacct cacaacaaaa cgttattggc gttttgttgt
     1981 gacaccgagg tcattatgac ggatgttatt cttgatgttt cgcatattgc gaagacattt
     2041 ggtcatgttc aagcattaaa agatattacc ctctctttac gtaaagggcg ggtgcatact
     2101 ttattaggcg agaatggtgc cggtaaatcg acattaatga aaatactggc tggtgtttat
     2161 ccgccaaccc agggaacgat aacactacgt ggtgaaacta tcaccattaa taatccgcag
     2221 cattcacgcc agttaggaat tgcgattatt tttcaggaat tgagcctgag taataatatg
     2281 accgtggcgg aaaatattta tgccaataat gaaccgcgac gttttggcat tattaacgat
     2341 aaaaaaatgc tcgcagattg ccagaatctc ctcgcggaac tgggcatacc actagaccca
     2401 ctagaaatgg tcggcaatat gtcgatggca cataggcaac tggtagaaat tgctaaagcc
     2461 ttaagctatg cagcggatgt cgtgattatg gatgaaccga catcttcgct aagtgataat
     2521 gaagcggaga ttctgtttaa cattattgaa aaattaaagc agcgaggctg tgcggttatt
     2581 tatatctctc atcgcatgga tgaaattatg cggatttcag atgatatttc ggtgatccgc
     2641 gacggtgagt atatcgccac gcatgaaaag aaaaacagtg atatccagca tttgattgcc
     2701 caaatggttg gtcgggaaat gaaaaatatt tggccagcac gtcttgggga aataccggat
     2761 gaaaatgttc ctgcaaaact ggaagtaaaa aatctttccc atccttcgct ttttaaagag
     2821 atcagtttcg ctgtgcgtcc gggggaggtc ctcggatttt ttggcctggt gggggccggg
     2881 cgttccgatg tgatgaaggc gctctttggc ctcgtttcgt atcaggggac tgtgttgatt
     2941 gatggtaagg aagtcaggat cgctaatcct aaacaggcta ttgatcatgg cattgctttt
     3001 gtgaccgaaa accgcaagga agaagggtta gtgctgatgc acgacgtcaa tatgaacacg
     3061 caccatgtgg cgtttcaata taacgcttcg cggatgggac tcatcaatca ccgacaagaa
     3121 gaggcgaaga cgctgcaatc aattgccaga atgaacacta aagtgagttc tgtacatcag
     3181 gcagtgggag cgctgagcgg cggtaatcaa cagaaaatcg tgttatcgaa gtggctggag
     3241 aaaactccgc gtattttatt gctcgatgag ccaacgcgcg gagtggatgt cggcgcaaaa
     3301 tttgaaatct ataacgtgat acgtcaactg gctgcggcag ggacagcaat tattctggtg
     3361 tcctctgaac tgccagaagt catggcactt agcgatcgtc tggtggtaat gcgtaataaa
     3421 acgattgccg atatttattc atgtgaaaat ttaacgcaga cacaggtaat gaccgctgca
     3481 acaggagtac gttaaatgac agagaaaact cttgccagtc cgaaaccttc ggaaattccc
     3541 attgcaaata attttaaaaa agtcttcagg caatatggcg gtatcctcag tggaatg
//
LOCUS       DAZFRJ010000299         3547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000299 DAZFRJ010000000
VERSION     DAZFRJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.301"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..110
                     /locus_tag="ACHKUN_004625"
     rRNA            <1..110
                     /locus_tag="ACHKUN_004625"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            205..320
                     /gene="rrf"
                     /locus_tag="ACHKUN_004626"
     rRNA            205..320
                     /gene="rrf"
                     /locus_tag="ACHKUN_004626"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(424..951)
                     /gene="mobB"
                     /locus_tag="ACHKUN_004627"
     CDS             complement(424..951)
                     /gene="mobB"
                     /locus_tag="ACHKUN_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO6662735.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(933..1517)
                     /gene="mobA"
                     /locus_tag="ACHKUN_004628"
     CDS             complement(933..1517)
                     /gene="mobA"
                     /locus_tag="ACHKUN_004628"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO6662736.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1587..1856
                     /locus_tag="ACHKUN_004629"
     CDS             1587..1856
                     /locus_tag="ACHKUN_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO6662737.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1933..2919
                     /gene="srkA"
                     /locus_tag="ACHKUN_004630"
     CDS             1933..2919
                     /gene="srkA"
                     /locus_tag="ACHKUN_004630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO6662738.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2936..>3547
                     /gene="dsbA"
                     /locus_tag="ACHKUN_004631"
     CDS             2936..>3547
                     /gene="dsbA"
                     /locus_tag="ACHKUN_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO6662739.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYL"
BASE COUNT          897 a          850 c          886 g          914 t
ORIGIN      
        1 ctttaagggt cctgaaggaa cgttgaagac gacgacgttg ataggccggg tgtgtaagcg
       61 cagcgatgcg ttgagctaac cggtactaat gaaccgtgag gcttaacctt acaacgccga
      121 agatgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
      181 agcggtctga taaaacagaa tttgcctggc ggccttagcg cggtggtccc acctgacccc
      241 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
      301 agagtaggga actgccaggc atcaaattaa gcagaaggcc atccgtcagg atggcctttt
      361 tgcattggcg cagaaaaaaa tgccggatgc gacgctggcg cgtcttatcc agcctactct
      421 tgcttatccg ttctgctttt gcatccactc caccacaaaa tcagccagcc cctcaacatc
      481 attgatatcc agtaatgcga catcaagatt aagcggcaca tcactggcta cagcaataac
      541 atgcctgtct atcactaatt cttcaggtcg atgtccggct ccatcgcgaa acagcacaat
      601 ctttgcgatc tcttcatgct taaacccttc gaccagaatc aaatccagct ttgaggtatc
      661 cattcgactt gcgagaaact gtagatccag ctcttcttcg tctggtgttt ccgtcatcaa
      721 ggcccatcgt tgctggctgg caacgatggt ttgtgccgcg ccagccttgc gcagctcata
      781 gctatctttg cctggcttat caacatccat atcatggtgc gtatgcttaa tcagccctgg
      841 acggatccct ctggcgcata atgccgggat cagttgtttc aaaagcgtag tttttccggt
      901 accactccac gcggcaaagg cgagtaacgg tatcatcgtt tttcctgcca tcgggcaagc
      961 tcctctggcg tattcacgtt aataaatgca tctttattat cgctgaaatc aaccgcatga
     1021 ccgccagcca gacgcataaa tgccattacc cggcgttctc ctgcttgcag atattccagt
     1081 aataaaggct caattgcgcg gtttaccaga gcaatagtcg ggtgatcgcg ttcaccgtcg
     1141 tggacccaca cgacaggcgc atctttgcgc tgatgattaa gccgggctgc taaatcctcg
     1201 ggaatataag gagtatcgca cggacaaaac aaaaaccact cgcctgcttc ctgctgcatt
     1261 actgaaagca ttcctgccag agggcctggg taatccgcca gtgaatcttc aatcactttc
     1321 agaccgcttg cctgatagat ttcctgatgg cgattggcat taaccacgac gtgagagagc
     1381 tgcgtcataa gcgcgtcagc gacatgttgc cataatggtt tgccgtttaa ttcaagcaat
     1441 cctttatcta cgccgcccat tcgtctggct ttaccgcctg ccagcacaac gcctgttatc
     1501 gtcgtcatca gattcaccga tatcgcctct tttattgtgg gattgaccct gctaacgtgt
     1561 ctgtctcaag agtaaggagc attactatga aatgtaaacg tctgaatgaa gttattgaac
     1621 tcctccagcc agcctggcaa aaagagccag accttaacct gttgcaattt ttgcagaaac
     1681 tggcgaaaga gtcaggtttt gacggcgaac tggcggattt gacggatgac attctgatct
     1741 atcacctgaa aatgcgtgat tccgcgaaag atgcggtgat cccgggtttg cagaaagatt
     1801 atgaagaaga cttcaaaacg gcgctgttac gcgctcgcgg cgtaattaaa gagtaaaagc
     1861 ttgtaagcgg tgccaccaaa atcatcgtga aatgatatcc ttcatcattc gtaatgtttt
     1921 ccggatgatg ggatgaacaa cagcgctttt actttccaga cactacaccc ggataccatc
     1981 atggatgctc tatttgagca agggatccgg gtggattccg gtcttacccc gcttaacagc
     2041 tatgaaaacc gtgtctatca atttcaggac gaagatcgtc gacgttttgt cgtcaaattt
     2101 tatcgccctg aacgttggac agccgatcaa atcctcgaag aacatcaatt tgcgttgcag
     2161 ttggtaaatg atgaagttcc ggtcgcagca cctgtggcct ttaacggtca gactttattg
     2221 aatcatcagg gattttattt cgctgttttt ccaagcgtcg gtggtcgcca gttcgaagct
     2281 gataatatcg atcagatgga agcggttggg cgttatttag ggcgtatgca ccagacgggg
     2341 cgcaaacagc tttttatcca tcgcccgacc atcggtctga atgaatatct cattgagcca
     2401 cgcaagctgt ttgaggacgc tacactgata ccttccgggt tgaaagcggc attcctgaaa
     2461 gcgacagatg agctgatcgc cgccgttaca gcgcactggc gggaagattt caccgttctg
     2521 cggctacatg gagactgcca cgccgggaat attctctggc gcgatggtcc aatgtttgtt
     2581 gatctggatg atgcacgtaa tggtccagct attcaggatt tgtggatgtt gctcaatggc
     2641 gataaagccg agcagcggat gcaactggaa actattattg aagcttatga agaatttagc
     2701 gagttcgaca ccgctgaaat cggactgatt gaacctttac gcgccatgcg tttggtttat
     2761 tatcttgcct ggctaatgcg gcgttgggct gatcccgcgt tcccgaaaaa tttcccgtgg
     2821 ttaaccgggg aagattactg gctgcgacaa acggcgactt ttatagaaca ggcaaaagtt
     2881 ctacaagaac cccctttaca attaacacct atgtattaat cggagagagt agatcatgaa
     2941 aaagatttgg ctggcgctgg ctggtttagt tttagcgttt agcgcatcgg cggcgcagta
     3001 tgaagatggt aaacagtaca ctaccctgga aaaaccggta gctggcgcgc cgcaagtgct
     3061 ggagtttttc tctttcttct gtccgcactg ctatcagttt gaagaagttc tgcatatttc
     3121 tgataatgtg aagaaaaaac tgccggaagg cgtgaagatg actaaatacc acgtcaactt
     3181 catgggtggt gacctgggca aagatctgac tcaggcatgg gctgtggcga tggcgctggg
     3241 cgtggaagac aaagtcactg ttccgctgtt tgaaggcgta cagaaaaccc agaccattcg
     3301 ttcagcatct gatatccgcg atgtatttat caacgcaggt attaaaggtg aagagtacga
     3361 cgcggcgtgg aacagcttcg tggtcaaatc tctggtcgct cagcaggaaa aagctgcagc
     3421 tgacgtgcaa ttgcgtggcg ttccggcgat gtttgttaac ggtaaatatc agctgaatcc
     3481 gcagggtatg gataccagca atatggatgt ttttgttcag cagtatgctg atacagtgaa
     3541 atatctg
//
LOCUS       DAZFRJ010000300         3508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000300 DAZFRJ010000000
VERSION     DAZFRJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.302"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..356
                     /locus_tag="ACHKUN_004632"
     CDS             <1..356
                     /locus_tag="ACHKUN_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6662740.1"
                     /translation="VLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKK
                     LLVNRSGNGLVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWY
                     LTIIDDHGNYISDKI"
     gene            372..2852
                     /gene="yehB"
                     /locus_tag="ACHKUN_004633"
     CDS             372..2852
                     /gene="yehB"
                     /locus_tag="ACHKUN_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6662741.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            2866..>3508
                     /locus_tag="ACHKUN_004634"
     CDS             2866..>3508
                     /locus_tag="ACHKUN_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO6662742.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLP"
BASE COUNT         1000 a          654 c          877 g          977 t
ORIGIN      
        1 atgttctgga tattccacca aatagtccag agcaagaagg caaaaatgca ctgaagtttg
       61 cgatgcaaaa cagaattaag ttgttttacc ggccagcggg tattgctccg gtaaataaag
      121 cgacctttaa aaagttactg gtaaatcgca gtggcaatgg attggtgata aaaaatgact
      181 cagctaattg ggtgacgatt tcggatgtca aagctaataa tgttaaagtc aattatgaaa
      241 ctattatgat tgccccctta gaaagtcaga gtgttaatgt caaaagtaat aatgcaaata
      301 actggtatct gaccattatc gatgaccacg gcaactatat tagtgacaaa atttaattgc
      361 aggagctgcc tatgttgaga atgaccccac ttgcatcagc aatcgtagcg ttattgctcg
      421 gcattgaagc ttatgcagct gaagaaacct ttgataccca ttttatgata ggtggaatga
      481 aagaccagca ggttgcaaat attcgtcttg atgataatca acccttaccg gggcagtatg
      541 acatcgatat ttatgtcaat aagcaatggc gcgggaaata tgagattatt gttaaagaca
      601 acccgcaaga aacatgttta tcaatagaag ttatcaagcg gttaggcatt aatagcgata
      661 acttcgccag cggtaagcaa tgtttaacat ttgagcaact tgttcagggt gggagctata
      721 cctgggatat cggggttttt cgtctcgatt tcagtgtccc gcaggcctgg gtggaagaac
      781 tggaaagtgg ctatgttcca ccggaaaact gggagcgggg tattaatgcg ttttatacct
      841 cttattatgt gagtcagtat tacagtgact ataaagcgtc gggtaatagt aagagtacat
      901 atgtacgttt taacagcggg ttaaatttac tggggtggca actgcattct gatgccagtt
      961 tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa taccctgtat ctggaacgtg
     1021 gatttgccca acttctcggc acgcttcgcg tgggtgatat gtacacatca agcgatattt
     1081 ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga tatgcagatg ttgcctaact
     1141 cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca gagtaatgcg ctggtaacta
     1201 ttgaacagaa cggttttgtg gtttatcaga aagaggttcc tcctggcccg ttcgcgatta
     1261 cagatttgca gttggccggt ggtggagcag atcttgatgt cagcgtgaaa gaggcggacg
     1321 gctcggtaac cacctatctg gtgccttatg cagcagtgcc aaatatgctg caacccggcg
     1381 tatcgaaata tgattttgcg gcgggtcgta gccatattga aggggcgagc aaacaaagtg
     1441 attttgtcca ggcgggttat cagtatggtt ttaataattt attgacgctg tatggtggct
     1501 cgatggtcgc gaataattat tacgcgttta ctttggggac tggctggaat acacgcattg
     1561 gtgccatttc cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc gatgtgtttg
     1621 acgggcaaag ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg
     1681 gtctggcggc ctggcgttat tcatcgcgtg attaccggac atttaatgat cacgtttggg
     1741 caaacaataa agataattat cgccgtgatg aaaacgatgt ctatgacatt gccgattatt
     1801 accagaacga ttttggccgt aaaaatagct tctctgccaa tatgagccag tcattgccag
     1861 aaggctgggg gtctgtgtcc ttaagtacgt tatggcgaga ttactggggg cgtagcggca
     1921 gcagtaagga ttatcagttg agttattcca ataactggcg gcggataagc tatatcctcg
     1981 cggcaagcca ggcgtatgac gagaatcatc atgaagagaa acgttttaat atttttatat
     2041 cgattccctt tgattggggt gatgacgtta cgacgcctcg tcggcaaata tatatgtcta
     2101 actcaacgac gtttgatgat caggggtttg cctcaaataa tacgggatta tcaggaaccg
     2161 ttggaagccg ggatcagttt aactatgggg tcaacctgag ttatcagaat cagggaaatg
     2221 aaacgacagc tggggcgaat ttgacctgga acgcgccggt tgcgacagtg aatggcagtt
     2281 atagtcagtc gagtacttat cgacaggctg gagccagtgt ttcagggggc attgtcgcct
     2341 ggtcgggggg cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc
     2401 caggaattaa agatgcttat gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag
     2461 tggtggtata cgacggaatg acaccttatc gggaaaatca cctgatgctg gatgtgtcgc
     2521 aaagcgatag cgaagcagaa ttacgtggca accggaaaat tgccgcctct tatcgcggcg
     2581 cggttgtact ggttaatttt gataccgatc agcgcaagcc ctggtttata aaagcgttaa
     2641 gagcggatgg gcaaccatta atgtttggtt atgaagtcaa tgatatccat ggtcataata
     2701 ttggcgttgt cggtcaggga agccagttat ttattcgcac caatgaagta ccgccatcgg
     2761 ttaatgtagc aattgataag caacaaggac tttcatgcac aatcaccttc ggtaaagaga
     2821 ttgatgaaag tagaaactat atttgccagt aatagtcagg tggttatgaa tattcgtata
     2881 atgttattta tagtaatgat gatggttatg cctgtgagct atgcggcatg ttatagtgag
     2941 ttatctgttc agcacaattt ggttgttcag ggggattttg cacttactca aacacaaatg
     3001 gcgacatatg agcataattt taatgattcg tcatgcgtga gtacaaatac tatcacccct
     3061 atgagcccgt cggatattat tgttggactt tataacgata ccattaaatt aaatttacat
     3121 tttgaatgga ccaataaaaa caacatcacg ttatcaaata atcagaccag tttcaccagt
     3181 ggttattcgg taacggtgac acctgcggcc agtaatgcaa aagtgaatgt ttctgcgggg
     3241 ggcggcggtt cagtgatgat taatggagtt gcgacattat ccagtgcttc atcatcgaca
     3301 cgcgggagtg ccgcagtaca atttctactg tgtttattag gtggcaagtc atgggatgca
     3361 tgtgtaaata gctacagaaa tgcattggca caaaatgcag gtgtctattc ctttaatctg
     3421 acattgtcat acaacccgat aaccacaacc tgcaaaccgg acgatttatt aattacttta
     3481 gacagtattc ccgtttcaca attaccag
//
LOCUS       DAZFRJ010000301         3482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000301 DAZFRJ010000000
VERSION     DAZFRJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.303"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            132..353
                     /locus_tag="ACHKUN_004635"
     CDS             132..353
                     /locus_tag="ACHKUN_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662743.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            784..1809
                     /locus_tag="ACHKUN_004636"
     CDS             784..1809
                     /locus_tag="ACHKUN_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6662744.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1877..2266
                     /locus_tag="ACHKUN_004637"
                     /pseudo
     CDS             1877..2266
                     /locus_tag="ACHKUN_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            2321..>3482
                     /locus_tag="ACHKUN_004638"
     CDS             join(2321..2609,2609..>3482)
                     /locus_tag="ACHKUN_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO6662745.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFCEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPA"
     misc_feature    2602..2718
                     /locus_tag="ACHKUN_004638"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          845 a          809 c          929 g          899 t
ORIGIN      
        1 tttttacaat tcacataatt tgtcattatt cattccgcaa tatttataac ctccggtgat
       61 aaaatggcat tcagctcgtt aataagagag ttaacttatt aagcgttagc gtttattact
      121 gaggtaacac catgaaaaga ttaattcctg tggcattgct caccgcattg ctggcgggct
      181 gtgctcacga ttcgccctgc gttccggttt atgacgatca gggtcgtctg gttcacacca
      241 atacctgtat gaaaggcacg actcaggata actgggaaac ggctggggct attgccggcg
      301 gggcagcggc tgttgctggc ctgacgatgg ggatcatcgc tctgtcgaaa taactgaaca
      361 gggcgcggag tttcgcgctc tgtttaacga tacagtatct ttacataaga taaaatattg
      421 ctatattctg ctggctaatt ctctgctgat ttagcatgtt atatcgcgtt atttcgctgc
      481 tttcgaaata acgcatcaat ttaactttca cacttcccac cagaaatata ctcacgctaa
      541 caatcattat tctgcttagt aaaacacgcg taaatatttt gcgccaaaat gtggcgcatg
      601 tttcattttc gcaccattgc ggggcgctgt ttttattatt tcctgtctac actcagctta
      661 ttgcgacgcg aaatcgggcg caaaagctgg cactactttt gcttataaga ggatggccac
      721 agacaggtaa aagacgtttc ccaaacgtcc tataacgata attttcgcct cacaggaagc
      781 attatgaaaa agatgatgat agccacactg gctgccgcca gcgtgctgct tgccgttgca
      841 aatcaggcgc atgctggagc gacgcttgat gccgttcaga aaaaaggttt tgtgcaatgc
      901 gggatcagtg atggattacc tgggttctct tatgccgatg cagacggtaa gttttcaggt
      961 attgatgttg atgtttgtcg tggtgttgcc gctgctgtat ttggtgacga cacgaaagtg
     1021 aaatataccc cactcacagc aaaagaacgc ttcacggctt tacagtcagg ggaagtggat
     1081 ttgctctccc gtaatacgac ctggacttca tctcgcgacg ccgggatggg aatggcattt
     1141 actggcgtca cttattacga cggcattggc ttcctgacgc acgataaagc ggggctaaaa
     1201 agcgcgaaag aactggatgg cgctaccgtc tgtattcagg cgggtactga taccgaactc
     1261 aacgttgccg actatttcaa ggcaaacaat atgaagtaca caccggtgac tttcgatcgc
     1321 tctgacgaat cagcgaaggc actggaatct ggtcgctgcg atacgctggc ctcggatcag
     1381 tcgcaactgt atgccctgcg catcaaatta agcaacccag ctgaatggat tgtcttaccg
     1441 gaggttatct ctaaagaacc gcttgggccg gtagttcgtc gtggcgatga tgaatggttc
     1501 tcgattgtac gctggacgct tttcgccatg ctgaatgctg aagagatggg catcaattcc
     1561 cagaacgtcg atgaaaaagc ggctaatcca gcaacgcctg atatggcaca tctgctgggt
     1621 aaagaaggcg attacggcaa ggatctgaag ttggataata aatgggccta taacatcatc
     1681 aaacaggtgg gtaactactc ggaaattttc gagcgtaatg taggttcaga aagcccgctg
     1741 aaaattaaac gtgggcaaaa taatctctgg aataacggcg gtattcagta cgcgccgccc
     1801 gtgcgttaag tcggtgtgag gtaacgagcg tcgctacggc ggcgctctct tcagcgttat
     1861 gctttccgag gtttctatgt ctcatcgccg ctcaaccgtt aaaggctcac tctcctttgc
     1921 caaccctacg gttcgtgcct ggttattcca gatccttgcc gttgttgctg ttgtcggcat
     1981 tgttggttgg ttatttcaca acactgtaac gaatctcagt aatcgtggca ttacttcagg
     2041 ttttgccttt ctggatcgcg gcgctggctt cggtattgtc cagcatttga tcgattacca
     2101 gcagggcgac acctacggac gcgtttttat tgtcggctta ctcaatacgc tactggtttc
     2161 tgcattgtgt attgtgttcg cttctgtttt gggcttcttt atcggtctgg cgagactttc
     2221 ggataactgg ctgctacgaa agctttccac aatttatatt gagatctgaa ccgccccgga
     2281 aatcctggag actaaactcc ctgagaaaga ggtaaacagg atgactaaaa atactcgttt
     2341 ttcccccgaa gtccgtcagc gggcgattcg tatggttctg gaaagtcagg atgaatatga
     2401 ctcacagtgg gcggcaattt gttccattgc cccaaagatt ggctgtacgc cggagaccct
     2461 gcgtgtctgg gttcgccagc atgagcggga taccgggggc ggtgatggtg ggctcaccag
     2521 cgctgaacgt cagcgtctga aagagctgga acgtgaaaat cgtgaactgc gccgcagtaa
     2581 cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc gcctctggaa
     2641 aaaatgatgc cactgctgga taagctgcgt gagcagtacg gggtcggacc ggtatgcagc
     2701 gaactgcata ttgccccgtc aacgtattac cattgtcagc aacagcgaca tcatccggat
     2761 aaacgcagtg cccgtgcgca gcacgacgac tggctgaaga gagagataca gcgcgtatac
     2821 gatgaaaatc atcaggtgta cggtgtgcgt aaagtctggc gtcagttgtt acgggaagga
     2881 atcagggtgg ccagatgtac agtggcacgt ctcatggcgg ttatgggact tgccggtgtt
     2941 ctccggggta aaaaggtccg tacgaccatc agccggaaag ccgttgccgc aggcgaccgc
     3001 gtaaaccgtc agttcgtggc agaacgacct gaccagctgt gggtggctga ttttacttac
     3061 gtcagcacat ggcagggctt cgtctatgtg gcgtttatca ttgatgtgtt tgccggatac
     3121 atcgtggggt ggcgggtctc atcgtctatg gaaacgacat tcgtgctgga tgcgctggag
     3181 caggcgttgt gggcccgtcg tccgtctggc accatccatc acagcgataa aggctctcag
     3241 tatgtgtcac tggcctatac ggagcgacta aaagaagccg gattactggc atcaacaggg
     3301 agtacaggcg actcgtatga caacgcgatg gctgagagca tcaatggtct ttacaaagcg
     3361 gaggtaatac accgtaagag ctggaaaaac cgtgcagaag tggaactggc cacactaacg
     3421 tgggtggact ggtataacaa tcgacgattg ctgggaaggc tgggccatac tcctccggca
     3481 ga
//
LOCUS       DAZFRJ010000302         3447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000302 DAZFRJ010000000
VERSION     DAZFRJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.304"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(330..1007)
                     /locus_tag="ACHKUN_004639"
     CDS             complement(330..1007)
                     /locus_tag="ACHKUN_004639"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO6662746.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1205..2824
                     /locus_tag="ACHKUN_004640"
     CDS             1205..2824
                     /locus_tag="ACHKUN_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO6662747.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            2821..>3447
                     /locus_tag="ACHKUN_004641"
     CDS             2821..>3447
                     /locus_tag="ACHKUN_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO6662748.1"
                     /translation="MKISTQVAEEPLSVVDTDDIFLLKQRLAEQEALIHALQEKLSNR
                     EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLHKESDTLTGRVYDPAVQR
                     PLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTTEQLELMRSAFRVIR
                     TVREKHACTQCDAIVQAPAPSRPIERGIARPGLLARVLTSKYAEHTPLY"
BASE COUNT          811 a          926 c          880 g          830 t
ORIGIN      
        1 cgccattctg agctcaatag tatccatgcc actgagctta caggtcttta tcgggcgata
       61 gtgctttatt cttcggggct ttgtggatga tttttcgaaa tgttcatgat agttaggtaa
      121 agctaccgca tatcatttgc aaaatgacag atagatatac ttcaatttga atcttaacaa
      181 ttagtatggg aatgctgtca atcctctgta tgcgtccacc acatattgaa atttttgttc
      241 tctaatatat tccacgggcg tcaagagtgt gaggcctgtg ctgttataca gcaccagacg
      301 ttgtccccca tctttgacct gtctcttgat cagatctcct gatcaagaga cttcatcacc
      361 aggtaaccct cagccatatc ctgatgtctg aaccagccat cccacataac tacccaaccg
      421 gggctgcttg tgcgtttgct gtcatgccat cgccccagtt tagccagttt caaacaggcc
      481 catttcaggg tcggtgtttg cgctggaagc tgctttcctt ccagcttaac ccacagtagt
      541 ttccactctg tcggcgtcag tattttctca cagctgtcat tttgtgtttc ttcactgata
      601 cttccctgcc gcaggcccag cacccgcacc gcgataacca ccatgcgctc aaggttatcc
      661 cgggtctgca ttcgcagcga ttccacacat gtaccaccac ttttccacgc tttgtggtat
      721 tcctctatca gccagcgttg ctcgtaatgg ctgacgatac gtcgcgcatc ggcggcactc
      781 gccacttttt ctgatgtcag cggataccag caggctccgt cctctcctgc tccccgcaac
      841 agacatacgt gagcgagagc gcctggccgc tgttgtcggg attttttatg ctgacttcgc
      901 tgtaactgat gagcatccgg gcctggcggg ctgcccgccc gcctttttgc atcacattca
      961 gcgtgtggct tcccgcggct gactttataa tgcaggtaat gccagatatc cgcttcccgg
     1021 tcacagacag tgattacctg tttctgtatc tcccccagcc gttcggccat gcgctccgaa
     1081 gcctgctgcc agcggtaact ttctttttct gtcacgaacg gtgcaatagt ggttcttccg
     1141 caacttgggt ggaagaaaat ggatgaaact ttctggtgaa caaagatcta agctgtttta
     1201 agtcatgggc aactcaatgc actctcttaa gacacttcta cagttacctt gcggatggcg
     1261 atgcagtcga caaattatta gctctgacgg tataaccctc catctccacg gaaaacgcaa
     1321 aaaagcacaa tgtcctgaat gctttaagcg tagcgactct gttcatagtt gtcgtcggcg
     1381 ccggatacag catctaccct gctcaggaca tacgctatgg cttgtatttt ccgtccgcca
     1441 ctggtactgc cgtaacccgg cttgttcacg taaaattttt gccgggtcgc ttgctccctt
     1501 cgccggttca caccaacagt cttcacaggc gttacaaaat ttacaacgtc aactgggatt
     1561 aatcgccgga ggtgaggccg ggaaacgggc agcaacggca gtggggctcc gttgcagtgc
     1621 tgatactctc cttcgcaggg ttatcaatac cccggagacg aaacagtcag gcgcgcctca
     1681 tgtcggtatt gatgagtggg cgtggcatcg gggccaccgt tacggtacgt taatcgtcaa
     1741 tcttgatact caccgtcccc tcgtcctgct tcccggccgt gatcagcgta cgctggcgac
     1801 ctggttcaga aaatatccgg aaatacaaat tgtctcgcgt gatcgcagtg gagtctatgc
     1861 gacagcagca cgtgaaggtg cacctcaggc cagacaggtg gccgatcgat ggcatctgct
     1921 aaaaaatatt ggcgatgcgc ttgaacgaat gatgtacaga catatacctc tgatacgtct
     1981 tgttgccagt gagttgtcac caaagaaatc acctgatcca gaaccatctg tgcctgcagc
     2041 atcgctccgt cgtccggaac gcctaaaaca acaaacccgc aaaaaacggc atcagcgttg
     2101 gacagaggtt atggccctgc ataacaagga atgtagtttc agggaaatat cccgtattac
     2161 aggcctgtcg cgtgtgacag tcagtcgctg ggtgcgttca ggaacattcc ctgaaatgtc
     2221 aacccgacct ccaaagcgag ggcttctgga cccatggagg gagtggttaa aagagcaacg
     2281 agaaagtagt aattataacg ccagccggat atggcgggaa atggtggccc ggggatttac
     2341 aggcagtgaa accatcgtca gggatgcggt tgccaaatgg cgtaaaggct ggaacccacc
     2401 ggttactact gccgtcagac ttccttcagt gtcccgggta agccgctggt tgatgccctg
     2461 gagaataacc agggatgaag aaaattatgc ttcccgattt attagtctga tgtgtgaaaa
     2521 agaacctgag ctgaaaatag cgcagcaact ggcactcgag ttctaccgta ttctgaaaac
     2581 ccaaaataaa tcacagctaa gcagttggtt caatcgagtc cacgaaagcg gttcagcaga
     2641 atttcggcgc gtggcggcgg ggatggaagc tgatgctgca gctatatgtg aggcaatcag
     2701 tagtcgctgg agtaatggtg ttgtcgaagg tcatgtaaat cgcctgaaga tgctgaaacg
     2761 ccagatgtat ggtcgagcag gatttgaact actcaggcag agggtaatga gtccactggc
     2821 atgaaaatct ccacccaggt tgcggaagag ccactttccg tcgttgacac tgacgacatc
     2881 ttcctgctga aacagcgcct ggccgaacag gaagcgctga tccacgccct gcaggaaaag
     2941 ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc agctggataa actccgccgg
     3001 atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat ggaagccgat
     3061 ctgaaccggc ttcataaaga gagcgatacg ctgactggta gggtgtatga cccggcagta
     3121 cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc ctgaatcact accccgtgac
     3181 gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc actgagctat
     3241 ctgggcgagg ataccaccga acagctggag ttgatgcgta gcgcctttcg ggttatccgg
     3301 acggtacggg aaaaacatgc ctgtactcag tgcgatgcca tcgtgcaggc acctgcacct
     3361 tcgcggccca tcgagcgggg tatcgccaga ccggggctgc tggcccgtgt gctgacctcg
     3421 aagtatgcag agcacacccc gctgtat
//
LOCUS       DAZFRJ010000303         3356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000303 DAZFRJ010000000
VERSION     DAZFRJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.305"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..1163
                     /gene="ompC"
                     /locus_tag="ACHKUN_004642"
     CDS             81..1163
                     /gene="ompC"
                     /locus_tag="ACHKUN_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6662749.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYTRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1352..1735)
                     /gene="quuD"
                     /locus_tag="ACHKUN_004643"
     CDS             complement(1352..1735)
                     /gene="quuD"
                     /locus_tag="ACHKUN_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO6662750.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1821..1961)
                     /locus_tag="ACHKUN_004644"
     CDS             complement(1821..1961)
                     /locus_tag="ACHKUN_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6662751.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1958..2320)
                     /gene="rusA"
                     /locus_tag="ACHKUN_004645"
     CDS             complement(1958..2320)
                     /gene="rusA"
                     /locus_tag="ACHKUN_004645"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO6662752.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2317..2607)
                     /locus_tag="ACHKUN_004646"
     CDS             complement(2317..2607)
                     /locus_tag="ACHKUN_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6662753.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2600..2770)
                     /locus_tag="ACHKUN_004647"
     CDS             complement(2600..2770)
                     /locus_tag="ACHKUN_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6662754.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2770..3225)
                     /gene="ybcN"
                     /locus_tag="ACHKUN_004648"
     CDS             complement(2770..3225)
                     /gene="ybcN"
                     /locus_tag="ACHKUN_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO6662755.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3222..3323)
                     /locus_tag="ACHKUN_004649"
     CDS             complement(3222..3323)
                     /locus_tag="ACHKUN_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662756.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
BASE COUNT          888 a          804 c          747 g          917 t
ORIGIN      
        1 aaataatcac attgattaga tgaatattta tcgcgcagtg acatcatttt ttaataatag
       61 ttcaaaaaaa agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat
      121 cagtactgat ggcgatgtct gctcaggcag ctgaaattta taataaagac agtaacaagc
      181 tggatctgta cgggaaagtt aatgctaagc actacttctc ctctaatgat gcagatgatg
      241 gtgatactac ttatacccgt cttggcttca aaggtgaaac ccaaatcaac gatcaactga
      301 ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg cgctgaatct caaggttcct
      361 ccaaagataa aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg
      421 attatggccg taactacggt gtagcatacg atattggtgc atggaccgac gttctgccag
      481 aattcggtgg cgatacctgg acccaaacag atgtattcat gactggtcgc accacaggtg
      541 ttgcaactta tcgtaacaat gacttctttg gtctggttga tggtctgaac tttgctgctc
      601 agtatcaggg taaaaatgac cgtactgacg taactcaagc taatggtgat ggtttcggtt
      661 tctccactac ttatgagtat gaaggtttcg gcgtaggtgc aacctatgct aaatctgatc
      721 gcactaataa tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg
      781 aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct actacctatt
      841 ctgaaaccca aaacatgact gtttttggta aaaaccatat tgccaacaaa gcacaaaact
      901 tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttatctgc
      961 aatctaaagg gaaagacttg ggtgcttggg gtgatcagga cctggttgaa tatattgatg
     1021 taggtgcaac ctattacttc aacaaaaata tgtccacttt tgttgactac aaaatcaacc
     1081 tgattgataa gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg
     1141 taggtatggt ttaccagttc taatttgatt actaaaacat atgttgtggg aggctttgcc
     1201 tccccaacat ataagtggct ccctcaagcc acttccttta gaagcacaac cttgcttcta
     1261 actatataaa ccttctgtta tatattaccc tttatttttg ggggcgtttc aacgccccat
     1321 tcttaataat ttttagtaaa caattggcat attaattaga gttattaaca acgatatcca
     1381 tctctaaccg gatatctaat gccattaaca tcccttcaat tatgccctca gccttctgta
     1441 accttttccc gatataacca tcagagcagc aatgcttacc tgccagtgac atgaatgtca
     1501 taccgactac ataataatct actaataaat cgtgcaaatc gctgttgttc tttttcagac
     1561 gggccatgca cccgcaaatg atcatcgcgt catcgtcaca acattgcggg cgagatttta
     1621 cttttgaagt aattaatccc ttaaaaccgg cggcaatgga cgaccaggtc acatcttcat
     1681 gattattagc cgcccacgct ccccaacgct caagaaccat ctgaatatca cgcatcaact
     1741 tactccacaa aaatcagacc agaacgccaa ttacaagcaa aaatcaacga aacagtatta
     1801 gttgattgtt atctctgact tcatactcct gctcctgtca gggttttggc gtaattcttc
     1861 agtattcggt aatcggtcaa aacagaaccg gggaaacgat ataagcgcag acgcccccag
     1921 cggtggcgaa gacgttctgc catataaaac tcaaacatca ttcattcccc atttcggtga
     1981 tggtcagttc cagcctccca cctttggtaa caggcatctt cacaacgcgg taatcaacga
     2041 cctgagcatc atccagccag aaacctgctt tagtgagtgc gtcaaaagcg gctttttgca
     2101 gattatccag gtcacggcga cggcgatccg gcatgtggca ctcaatgcgg attttcacag
     2161 gcatagccaa gccgatatcc agcattgcgt ttttaatgat tcgggcgacg ttatcgcggt
     2221 atgcctgccc ctctgcgctg acgtgcgtgc gcccgcgatt atggcggtaa tagcgattat
     2281 tgctcggagg ccagggtaat gtgatgctgt aggtattcac gccttgatta ccccctcttt
     2341 cagccagata acctgcgttc tcgccatacc ttccagcgcg cattcttttg cgtactcagc
     2401 atcgacaaaa tgcgtgcggc ggtcgatttc gtcgtgacag gcagaacagg caatggtggc
     2461 aataaggtct ggcggtttaa taccggtgcc acacagtcca gccagccgga tatgtgccag
     2521 tacagacgtt tcagggttgc cattgcatac gccagggatt cttacctggc attcgcgacc
     2581 acgcgctgct tttctcaaat cagccatgat tcctccttgc tgccagtcgc aaccattttt
     2641 tatcaaccag gctggcggta tatccgagca gtgttggtat ttcggatggt tttagctcag
     2701 gcttacgctt acgacgattt ggtactctgt agatgtgtcc gttcatgaca cgaataagcg
     2761 gtgtagccat tacgcctcct gcttgtcgcg cagcagctgg aactcgcagc tctgcggaat
     2821 agtcaggtgg cagccaatat tcatcgccca ggcttcaacc ttacacagga agacatacat
     2881 ctctccggta tcaagatcgg aggtatggcg taacgactgg atagtagtga tttcgccggt
     2941 tacgacatca accaggtcct tggtttcata accgaggtat gtgtgtttga gagcatcttt
     3001 tacccatgct gcggtagcga acgatttccc cctgcggata aggtattcac tgatttcgct
     3061 gtaccacatg tggctaagtg cattctggga aagactgcgt ctctcgcgcc acggtttaag
     3121 caccatgcga aagcacttgc cgtcttccaa ataaggctgg agctgctgac cgatagcggt
     3181 gaagttaccg cgatgcagtt tgatgccgtc ttgtgggagg ttcacgcttc acctccgcag
     3241 aggtcaaacg ctggataaag aaaatcgcag gtgcatttct gcatctgtga cgggagaaga
     3301 tagtttagat tgtatgtgcg cataaacgtc cccgtttagc gcagaagtca ccggag
//
LOCUS       DAZFRJ010000304         3311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000304 DAZFRJ010000000
VERSION     DAZFRJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.306"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            186..1244
                     /gene="rfaQ"
                     /locus_tag="ACHKUN_004650"
     CDS             186..1244
                     /gene="rfaQ"
                     /locus_tag="ACHKUN_004650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO6662757.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1241..2365
                     /locus_tag="ACHKUN_004651"
     CDS             1241..2365
                     /locus_tag="ACHKUN_004651"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6662758.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            2358..3155
                     /gene="rfaP"
                     /locus_tag="ACHKUN_004652"
     CDS             2358..3155
                     /gene="rfaP"
                     /locus_tag="ACHKUN_004652"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO6662759.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          872 a          720 c          812 g          907 t
ORIGIN      
        1 atttctttat tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa
       61 gaagtgatac actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc
      121 ctggggcggt agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt
      181 tacttgtgga taagccattt cgaagaattt tgctcattaa gatgcgtttt catggggata
      241 tgttattaac tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg
      301 atgtgctgct ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc
      361 tctacggcat aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac ttttttcatc
      421 tcatcaaggt attacgtgcc aataagtatg accttatcgt caatctcacc gatcaatgga
      481 tggttgctat actggttcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc
      541 atcggcagtc tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa
      601 atgtggtgga aagtaactta tccgtgctga ccccattggg agttgattcg ttggtgaagc
      661 agacaaccat gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcacg
      721 ctggtgttgg acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct
      781 gggacaacgc caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagtcg
      841 ttctgacgtc cggcccagat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat
      901 gccagacgcc accagtaacg gcgctggctg gaaaggtgac cttcccggaa cttggtgcgt
      961 taatcgatca tgcgcagctg tttattggcg ttgattccgc accggcgcat attgccgctg
     1021 cagttaatac gccgctgata tcgctgtttg gtgcgacaga tcatattttc tggcgtccct
     1081 ggtcaaataa catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc
     1141 agcgtgaccg aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgctgctg
     1201 tcgataaatt actgccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt
     1261 atataaatat tttccctttg gcggtttgca gcgtgatttt atgcgtattg ctcagacagt
     1321 cgccgcccga ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg aatgccctga
     1381 tgtatttgaa ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga
     1441 gtattttgcc tgggtgcaaa aacatttacg cgaacatccc gtcgataaag tcgttggatt
     1501 caacaaaatg ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt
     1561 agcgcaggaa aaaggctttt tctatcgcct gacgtcacgt tatcgccatt atgccgcctt
     1621 tgagcgggca accttcgaac agggtaagcc gacacagctg ctgatgctga cagataagca
     1681 aatcgccgat ttccagaaac attatcagac tgaagcggag cgttttcata ttctgccacc
     1741 ggggatttat cctgatcgta aatatagcca gcagccagcc aatagccgtg aaatcttccg
     1801 taagaagaat ggaataaccg aacaacaata tttattgttg caggtcggtt cagacttcac
     1861 gcgtaaaggt gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca
     1921 caacacattg ctatatgttg ttgggcagga taaaccgcga aaatttgagg cgctggcaga
     1981 aaaacgcggc gtgtgcagta atgttcactt tttctcgggg cgcaatgatg tctcggaatt
     2041 aatggcggcg gcggatttat tactgcatcc tgcctaccag gaagcggcgg gaattgtgct
     2101 gctggaagcg attactgcag gattaccggt actaacaaca gccgtttgtg gctatgcgcc
     2161 ttatattgtc gaggctaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt
     2221 gaatgagatt ttacgcaaag cgttaacgca atcttcattg cgccaggcct gggcggaaaa
     2281 tgcgcgatat tatgctgata cacaagattt atacagtctg ccagagaaag cggcggatat
     2341 cataacgggt ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggca
     2401 aagatccttt tgaggaagtt aaaaccttgc agggtgaggt atttcgtgaa ctggaaactc
     2461 gccgtactct gcgctttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca
     2521 cgaccctgaa agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag
     2581 accgcgaatg gaatgcgatt catcgactgc gggatgtcgg cgttgatact atgcatgggg
     2641 tggcatttgg cgaaaaaggc atgaatccgc tgaccagaac ttcatttatt attaccgaag
     2701 atctgacacc aaccataagt ctggaagatt actgtgctga ctgggcgact aaccctccag
     2761 atgttcgcgt aaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg
     2821 ctgcgggcat taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt
     2881 ccggtaagga agaggagtta aaaatttcgg taattgacct gcaccgggcg cagcttcgca
     2941 cgcgcgttcc acgtcgttgg cgggataaag atcttattgg gctttatttt tcttcgatga
     3001 atatcggcct gactcagcgg gatatctggc ggtttatgaa agtgtatttt gccgccccgc
     3061 ttaaagacat tctcaagcag gaacaaggac tgctgtcgca agcagaagca aaagccacaa
     3121 aaatcaggga aagaacgatt cgaaaatcgt tgtaacccgt aacttatttt tgccaaaatt
     3181 ttggatacag aataaatatg tctcaactca atgatagtga catcatcctt tttgagtata
     3241 attttcatta tcaaaatata agatctaaaa atactcttga tatagcattt ggtattgaca
     3301 gaaattttct t
//
LOCUS       DAZFRJ010000305         3305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000305 DAZFRJ010000000
VERSION     DAZFRJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.307"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            338..1654
                     /gene="shiA"
                     /locus_tag="ACHKUN_004653"
     CDS             338..1654
                     /gene="shiA"
                     /locus_tag="ACHKUN_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO6662760.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1756..3210
                     /gene="amn"
                     /locus_tag="ACHKUN_004654"
     CDS             1756..3210
                     /gene="amn"
                     /locus_tag="ACHKUN_004654"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO6662761.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          760 a          811 c          797 g          937 t
ORIGIN      
        1 ctcaacaatg atactaaaaa cataactaac ctcgcctccc tactggttat aatgcaatgc
       61 agtctatcag aatcatcagg gtgcaatttt gtgcatatgc ggacttttat gtttcatatc
      121 tcgaacctgt gggccctctg cttaatcctt aaacaacacc agcaactcct gcgcttttat
      181 cttccatcga atttttcatg ttgccgctaa tcagccataa aatcatttgc agatgcgctc
      241 tgtcgaggta gtctcataag gttcgtttat agatcgacgg caatgtgagt taccttttcc
      301 atactaatta taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac
      361 tcgtcccgat gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt taggcagttt
      421 cgctggtgcc gtcgtcgact ggtatgattt tttactctat ggcattaccg ccgcactggt
      481 gtttaatcgc gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg ccgcatttgc
      541 cacctttggt gtcggcttcc ttttccgacc gctcggcggt gtcattttcg gtcactttgg
      601 tgaccggctg ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg gcatcgcgac
      661 agccttgatt ggtattcttc cttcattctc gaccattggg tggtgggcac ctattttgct
      721 ggtgacactg cgtgccattc agggatttgc cgtcggcggc gaatggggag gcgcagcgtt
      781 gctttccgtt gaaagtgcgc cgaaaaataa aaaagccttt tacagtagcg gtgtacaagt
      841 tggctacgga gtaggtttac tgctttcaac cggactggtt tcattgatca gtatgatgac
      901 gactgacgta cagtttttaa gctggggctg gcgcattcct ttcctgttta gcatcgtact
      961 ggttctggga gcattgtggg tgcgcaatgg catggaggag tccgcggaat ttgaacaaca
     1021 gcaacataat caagcggccg cgaaaaaacg catcccggtt atcgaagctc tgttacgaca
     1081 tcccggtgct ttcctgaaga ttattgcgct acgactgtgc gagttgctga cgatgtacat
     1141 cgttaccgcc tttgcactta attattcaac ccagaatatg gggttaccgc gcgaactttt
     1201 ccttaatatt ggtttgctgg taggtggatt aagctgcctg acaattccct gttttgccag
     1261 gcttgccgat cgttttggtc gccgcagggt ttatatcaca ggtgcgttaa tcggaatgtt
     1321 gagcgcattt cctttcttta tggcgcttga agcacaatct attttctgga tagttttctt
     1381 ctccataatg ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt
     1441 taccgaaatg tttggtgcca gttatcgcta tagtggcgct ggagtcggtt atcaggttgc
     1501 cagtgtggtt ggtggtggat ttacaccttt tattgccgct gcactcatca cttactttgc
     1561 cgggaactgg catagcgtcg ccatttattt gctggctgga tgcctgattt ccgcaataac
     1621 cgctttgttg atgaaagaca atcaacgcgc ttgatagcct ggcgaagatc atccgatctt
     1681 cgccttacac ttttgtttca cattcctgtg acatactatc ggatgtgcgg taattgtatg
     1741 gaacaggaga cacacatgaa taataagggc tccggtctga ccccagctca ggcactggat
     1801 aaactcgacg cgctgtatga gcaatctgta gttgctttac gcaacgccat tggcaactat
     1861 attacaagtg gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca
     1921 tcactgaccg taacctggga cggtagcaca accaatcccc ctaaaacgcg cgcatttggt
     1981 cgttttactc acgcaggcag ctacaccacc acgattactc gccctactct ctttcgttcg
     2041 tatctcaatg aacaacttac gttgctgtat caggattatg gtgcgcatat ctcggtgcaa
     2101 ccctcgcagc atgaaatccc ttatccttat gtcattgatg gctctgaatt gacacttgat
     2161 cgctcaatga gcgctgggtt aactcgctat ttcccgacaa cagaactggc gcaaattggc
     2221 gatgaaactg cagacggcat ttatcatcca actgaattct ccccgctatc gcattttgat
     2281 gcgcgccgcg tcgatttttc cctcgcgcgg ttgcgccatt ataccggtac gccagttgaa
     2341 cattttcagc cgttcgtctt gtttaccaac tacacacgtt atgtggatga attcgttcgt
     2401 tggggatgca gccagatcct cgatcctgat agtccctaca ttgccctttc ttgtgctggc
     2461 gggaactgga tcaccgccga aaccgaagcg ccagaagaag ccatttccga ccttgcatgg
     2521 aaaaaacacc agatgcccgc gtggcattta attaccgccg atggtcaggg tattactctg
     2581 gtgaatattg gcgtgggacc gtcaaatgct aaaaccatct gcgatcatct ggcagtacta
     2641 cgcccggatg tctggttgat gattggtcac tgtggtggct tacgtgaaag ccaggccatt
     2701 ggcgattatg tacttgcaca tgcttattta cgcgatgatc acgttcttga tgcggttctg
     2761 ccgcccgata ttcctattcc aagcattgct gaagtgcaac gtgcgcttta tgacgctacc
     2821 aagttagtca gcggcaggcc cggtgaggaa gtcaaacagc ggctgcgtac aggtactgtg
     2881 gtaaccacag atgacaggaa ctgggaatta cgttactcag cttctgcact tcgttttaac
     2941 ttaagccggg ccgtggcaat tgatatggaa agtgcaacca ttgccgcgca aggatatcgt
     3001 ttccgcgtgc catacgggac actactgtgt gtttcagata aaccgttgca tggcgagatt
     3061 aaacttcccg gccaggctaa ccgtttttat gaaggcgcta tttccgaaca cctgcaaatt
     3121 ggcattcggg caatcgattt gctgcgcgca gaaggcgacc gactgcattc acgtaaatta
     3181 cgaaccttta atgagccgcc gttccgataa taagttctga accttcttat caaaaataga
     3241 tgtgaaggat taagtaagac atctggcaaa tagcccgcaa taaacgcggg ctgtttgctt
     3301 ccttg
//
LOCUS       DAZFRJ010000306         3300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000306 DAZFRJ010000000
VERSION     DAZFRJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.308"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            232..1164
                     /gene="rfaD"
                     /locus_tag="ACHKUN_004655"
     CDS             232..1164
                     /gene="rfaD"
                     /locus_tag="ACHKUN_004655"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO6662762.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHKUN_004656"
     CDS             1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHKUN_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO6662763.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHKUN_004657"
     CDS             2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHKUN_004657"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO6662764.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3213..>3300
                     /locus_tag="ACHKUN_004658"
     CDS             3213..>3300
                     /locus_tag="ACHKUN_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662765.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITT"
BASE COUNT          838 a          808 c          863 g          791 t
ORIGIN      
        1 cattcaaatg cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac
       61 atgcaaaacc aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt
      121 agcatccttg cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca
      181 gacattcgtg tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc
      241 gttaccggcg gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc
      301 atcaccgata ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg
      361 gatctgaata tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc
      421 gaagagttcg gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag
      481 tgggacggca agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac
      541 tgcctggagc gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc
      601 acctccgact ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca
      661 aaattcctgt ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt
      721 ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc
      781 gtcgctttcc atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt
      841 agcgagaact ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg
      901 ttcctggaaa atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc
      961 caggctgtag ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg
     1021 ttcccggata agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg
     1081 cgcgcggcgg gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg
     1141 gcctggctga atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct
     1201 tgggttggcg acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc
     1261 caggcgataa tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg
     1321 gaagttaacg aagctatccc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc
     1381 cgcaaactgg gtcatagcct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac
     1441 tccttcaaat ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc
     1501 ggcgagatgc gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg
     1561 ctaatggtgg aacgctatat agcgctggcc tatgacaaag gcattatgcg cacagcacaa
     1621 gatctgccgc agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat
     1681 acctgtaatc aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg
     1741 gagtttggtc cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg
     1801 attgatgaag gttatcaggt ggttctgttt ggctcggcga aagatcatga agcaggcaat
     1861 gagattcttg ccgctttaaa taccgagcag caggcatggt gtcggaacct ggcgggggaa
     1921 acacagcttg atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat
     1981 tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtcca
     2041 agtagcccgg acttcacacc gccgctatcc cataaagcgc gcgtgatccg tctgattacc
     2101 ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac
     2161 attactcccc agcgcgtact ggaagaactc aacgcgctat tgttacaaga ggaagcctga
     2221 cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc
     2281 gcactcactg atgcccagca ggcaatccca gggattaagt ttgactgggt ggtggaagaa
     2341 gggttcgcac agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata
     2401 cgtcgctggc gtaaagcctg gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt
     2461 gaagcgctac aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc
     2521 gcggcactgg tgacacgtct ggcgcatggc gtaaagcatg gattggactg gcaaaccgct
     2581 cgcgaacctt tagccagcct gttttacaat tgtaagcatc atattgcaaa acagcagcac
     2641 gccgtagaac gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc
     2701 cagggcgatt atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa
     2761 tatgccgtat ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg
     2821 cgagaattga ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg
     2881 ccgcatgagg aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg
     2941 ccgaagatga gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg
     3001 gtggatacgg ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat
     3061 ggaccaaccg atccgggatt aattggtggg tatgggaaga atcaagtaga gtgtcgttct
     3121 acaagtatgt ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa
     3181 ataataacca ataagttgac atcggagata agatgacctc aacattattt ttctctctcg
     3241 agaaaaaaaa ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct
//
LOCUS       DAZFRJ010000307         3280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000307 DAZFRJ010000000
VERSION     DAZFRJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.309"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(40..759)
                     /gene="dcuR"
                     /locus_tag="ACHKUN_004659"
     CDS             complement(40..759)
                     /gene="dcuR"
                     /locus_tag="ACHKUN_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO6662766.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(756..2387)
                     /locus_tag="ACHKUN_004660"
     CDS             complement(756..2387)
                     /locus_tag="ACHKUN_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6662767.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2568..2798
                     /gene="yjdI"
                     /locus_tag="ACHKUN_004661"
     CDS             2568..2798
                     /gene="yjdI"
                     /locus_tag="ACHKUN_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO6662768.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2810..3082
                     /locus_tag="ACHKUN_004662"
     CDS             2810..3082
                     /locus_tag="ACHKUN_004662"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6662769.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          834 a          767 c          824 g          855 t
ORIGIN      
        1 tacggtaata attattactt acagataacc agcgatccgt tattggcaat attgtttcag
       61 tagtgagtag tgttctgcct gaatacggta acggtaaacc ggacgccccg tgacgccata
      121 atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt tacggcagga
      181 aacacgcgag atgttaacct cgttagccag ctcgtcggtt gaaaactcat agtcctgatg
      241 cgcgtcaatc cactggcaca gcgtgcgtaa agtctgcggc gttaagcctt ttggcaagcg
      301 acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct cggcctggtc
      361 ataatactga tgtttttcca gcgccatttt cttttgccgc cagccggtga gcgcctcttc
      421 aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat gcagcgaatc
      481 tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt tgcaacgcgc
      541 gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga tatcgagcaa
      601 tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct ccagcgtcga
      661 ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat tcagctccgc
      721 gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct ctccccgtcc
      781 cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac ggcgatgctg
      841 ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg ctcgcttcct
      901 tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat tcccggtcca
      961 tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac gctaatttcg
     1021 ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc caatgtggta
     1081 atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gctggctttc actgtttaaa
     1141 atcagcgtat ggcctaaatc ggtcgcgcgg ttaatcttgc tgattaaaaa accagcgata
     1201 accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt attggctgtt
     1261 ttgagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc gagaatcaca
     1321 tgcaatttat tcataaattc gtgggatcgt tcacgaagtg catcagcata gttgaccaga
     1381 ccgtcgagtc gctgcatcag tttacgtact tcagttttgt ccctgaaggt tgaaatggca
     1441 ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag ccggtcttta
     1501 atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga gacatctacc
     1561 acctgtgacc atgagtggct gagcgtcgac agtttctcat cgtcctgcga cttacggtaa
     1621 ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc gtccacggca
     1681 acgacgcctt ctttgataga ctgcaacatg gcctggcgct gctcaaacag cgtggagatt
     1741 tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat gcaggtgcca
     1801 atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg actgtcattg
     1861 atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat ttgtttatga
     1921 ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc gcgattgata
     1981 gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa tggctgacca
     2041 atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac gacaataaac
     2101 agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggattccgct ctcctgcggt
     2161 tttttctgca agccctgacg gatttccggc gagtcggcga gggtacgcgc cactgccagt
     2221 gccttgttgg ccagcccgtc tcgcgtcata tcactgattt gcgagaagta aatcagatgc
     2281 accaccaata gcaccgagaa cagtaccgcg ctgaccatta agatcactgt ggtactcaat
     2341 ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag cttccttgtg
     2401 tgacaaattt cttaagcatt atctctgatg aggcgggtaa ttcaaaggga gtaagaatga
     2461 ttggctatat aggggaagag actctggcaa cggaaactgc cagtgctgta tgaagattcc
     2521 agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg gatcaggcgc
     2581 tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat ttcaacactg
     2641 cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt aatctcaaac
     2701 gaaagccgtg gatcatgccg gatgaagtcg acgtcgtcac tgtggttaaa gtgattgata
     2761 cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa tggaaatacg
     2821 cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg ctgaaattgt
     2881 ctttgtgccg accggagaga atttagcgat tatcgaacat accgatgtcg atgaaagcct
     2941 gaaagggcag gggattggta aacagctggt tgcgaaagtc gtggaaaaaa tgcgtcggga
     3001 aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata aaacgcggga
     3061 gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt catgctctcc
     3121 gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat gggcagagag
     3181 cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata tctcctatat
     3241 gagaatcatc aatcggggtt aataattttt gcgtccccag
//
LOCUS       DAZFRJ010000308         3241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000308 DAZFRJ010000000
VERSION     DAZFRJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.310"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..926
                     /gene="mepH"
                     /locus_tag="ACHKUN_004663"
     CDS             99..926
                     /gene="mepH"
                     /locus_tag="ACHKUN_004663"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO6662770.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1054..1635
                     /gene="sodB"
                     /locus_tag="ACHKUN_004664"
     CDS             1054..1635
                     /gene="sodB"
                     /locus_tag="ACHKUN_004664"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO6662771.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1781..2950)
                     /gene="ydhP"
                     /locus_tag="ACHKUN_004665"
     CDS             complement(1781..2950)
                     /gene="ydhP"
                     /locus_tag="ACHKUN_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6662772.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3116..3205)
                     /gene="cydH"
                     /locus_tag="ACHKUN_004666"
                     /gene_synonym="ynhF"
     CDS             complement(3116..3205)
                     /gene="cydH"
                     /locus_tag="ACHKUN_004666"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO6662773.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
BASE COUNT          942 a          857 c          729 g          713 t
ORIGIN      
        1 aaaagctatt aactttctct tcttctatgc attagaatca tcaagttttg taaatcagac
       61 gcaggcatga tagacctgcc ttaacagagg gacgctcagt ggcgcggata aaccgtattt
      121 cgatcacgct ctgtgcttta ctttttacca ccctgccttt aacgcctatg gcccatgctt
      181 caaagcaagc cagggagagt tctgctacca ctcatatcac caaaaaagca gataaaaaga
      241 aaagcacggc aaccaccaaa aagacccaaa aagccagcca gaaaacagcg aaaaaagccg
      301 ccagtaaaag tacgaccaaa agcaaaaccg cttcttccgt taaaaaatct tccattaccg
      361 cttctaaaaa cgccaaaact cgcagcaaac acgccgtcaa taaaacggcc tcagccagct
      421 ttaccgaaaa gtgtaccaaa cgtaagggct ataaatcgca ttgtgtgaaa gtcaaaaatg
      481 ccgcgtcagg aactcttgcc gacgcgcaca aagcgaaggt gcaaaaagct acgaaagtgg
      541 caatgaataa actgatgcag caaattggta agccatatcg ttggggtggc agctcaccgc
      601 gtaccggttt tgattgcagc ggcctggttt attacgctta taaagatttg gtgaaaattc
      661 gtattccgcg tacggcgaat gaaatgtatc acctgcgtga tgcagcgcca atcgaacgta
      721 gtgaactgaa aaacggcgac ctggtctttt tccgtactca gggacgcggc acagccgatc
      781 atgtcggcgt gtatgtcggc aacggcaaat ttattcagtc accgcgcaca ggtcaggaaa
      841 ttcaaatcac ttctctcagt gaagactact ggcagcgcca ctatgttggc gctcgtcggg
      901 taatgacccc aaaaacactt cgctaaaact ttaccctgtt gttacggcaa cagggtaagt
      961 tcatcttttg tctcaccttt taatttgcta ccctatccat acgcacaata aggctattgt
     1021 acgtatgcaa attaataata aaggagagta gcaatgtcat tcgaattacc tgcactacca
     1081 tatgctaaag atgctctggc accgcacatt tctgcggaaa ctatcgagta tcactacggc
     1141 aagcatcatc agacttatgt cactaacctg aacaacctga ttaaaggtac cgcgtttgaa
     1201 ggtaaatcac tggaagagat tattcgcagc tctgaaggtg gcgtattcaa caacgcagct
     1261 caggtctgga accatacttt ctactggaac tgcctggcac cgaacgccgg tggcgaaccg
     1321 actggaaaag tcgctgaagc tatcgccgca tcttttggta gctttgccga tttcaaagcg
     1381 cagtttactg atgcagcgat caaaaacttt ggttctggct ggacctggct ggtgaaaaac
     1441 agcgatggca aactggctat cgtttcaacc tctaacgcgg gtactccgct gaccaccgat
     1501 gcgactccgc tgctgaccgt tgatgtctgg gaacacgctt attacatcga ctatcgcaat
     1561 gcacgtcctg gctatctgga gcacttctgg gcgctggtga actgggaatt cgtagcgaaa
     1621 aatctcgctg cataataact gatggcaaat gcagcattgc ctgatgcgct tcgcttacca
     1681 tgccaacgcg gatgaggcgt taacagcgca ttcggcaaga taaatcgcac gttgtcagca
     1741 actgtaacgc agaaggttat ccttctgcgt ttttgtttaa ttagctgtta gcaacgcaaa
     1801 ctgtttcagg ttgttttctg gctgacataa acaccagcaa taatgccagt cccgcgacaa
     1861 tcgctcccat caccggcaca aagctgtatc ccagcccagc ggaaattacc gcaccaccag
     1921 cagctgctcc cagcgcattt ccaagattaa aggcaccaat attgactgat gaagacagac
     1981 ctggcgcttc actggcgaca cgcatcacgc gcatctgtaa cggcggtacg accgcaaagg
     2041 ttgctgcgcc ccacaccacc atgctaatag ctgcgccgaa ctcattgcgg gccaggaacg
     2101 ggattgccag cataatcacc atcaacaaca acaaaaagcc tttcaacgtg ccgttaactg
     2161 aacgatctgc cagtttgccg ccgagatagt taccgataga gaatccgaca ccaatcagca
     2221 ccagcattgc cgtgacgaac actggtgttg cgtgggtaat actttgtagt accggagaga
     2281 tataggtgta gagagtaaac attgcaccag ctcccagtac cgtcgtcagc aatgcagaca
     2341 gcacctgcgg acgcattaat accgccagct cttttttcac ttcaggtcgt gcccctgcac
     2401 cacctttagg taatgagaag aacagactta ccattgaaat cactcccagc cccgccgttg
     2461 ccagaaatga catccgccag ccgatggttt cacccaacca ggtcgccgcc ggcacgccac
     2521 cgatatttgc cagggttaac cccataaaca tagtggcaac tgcgctggcc tgtttatgtt
     2581 ttggcaccac gcttgcggcc acgactgaac ccaaaccaaa aaatgctccg tgattcaggc
     2641 tggtcaaaat gcgtgaaagc atcagggtca tataatccgg cgcgatggcg gaaagtacgt
     2701 tgccgagcgt gaaaattgcc atcaggaaaa tcaacgcact gcggcgggca cgatgagaaa
     2761 gtagaagcgt catcagcggc gcgccaacca ttacgccaac tgcataggca ctgattaaca
     2821 ttccggcagc gggaatcgag acatccacac cgcgcgcaat gacgggcaac aaacccattg
     2881 gagagaactc cgttgtcccg ataccaaacg cgccaatcgc cagcgccagc aacggatagt
     2941 taattttcat gccttatctc cacctcttcg cgtcattacg cgatattcat taaagtggcg
     3001 aaagcatgac agcaatcaca aaaaaatgaa aataacaaaa agagaaaaca cttttgccat
     3061 tttgctaaca aacaggaagg agatgcgagg gagaacgcgc tccctcgaga ggaaatcagt
     3121 gcagcgcggc agtcaaaccc acggccacga tcaaaccgag gacgataatc gttgttacca
     3181 gtgaaaattt aaggtcggtg ctcatcaagt tttctccttt tttattacca cacaaatagt
     3241 g
//
LOCUS       DAZFRJ010000309         3216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000309 DAZFRJ010000000
VERSION     DAZFRJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.311"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(133..399)
                     /locus_tag="ACHKUN_004667"
     CDS             complement(133..399)
                     /locus_tag="ACHKUN_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662774.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENIPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            complement(492..926)
                     /locus_tag="ACHKUN_004668"
     CDS             complement(492..926)
                     /locus_tag="ACHKUN_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO6662775.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(1654..2154)
                     /locus_tag="ACHKUN_004669"
     CDS             complement(1654..2154)
                     /locus_tag="ACHKUN_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO6662776.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(2616..3213)
                     /locus_tag="ACHKUN_004670"
                     /pseudo
     CDS             complement(2616..3213)
                     /locus_tag="ACHKUN_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          690 a          980 c          922 g          624 t
ORIGIN      
        1 ttcgccagga acgaccagaa cgcggcatca tgcggcgtgg gcgttgtggt gtttttcttc
       61 ttactcacgg ttcctcctgg tgagatgtct gaacgtatcc tgccgcctgc acaggcagac
      121 ggcaagggtt aatcagtggc aggcccgctc aatttcaagc gcgcggagca ggtggcccca
      181 ttcgctgact ttcatggcgg ggatacgtac ccgcttctgc tcactgcagc gtctggcgat
      241 atgaccgctc atcgccagga cgtccgggtt aatctcatcc tgtgccagct cctgacgtct
      301 gacgcgggta tggcggtgca ggggtatatt ctcccgcagg cactcttcac gcagacgctt
      361 tacatactcg gcggttgcga agataatgga tggtctcatg gcataatctc catagatagt
      421 gattgagatg ccggacttcc gtacggggaa gcgccggcgg agaaaggcag ggtttatcgc
      481 cctgcctttt ttcatttcgg acagcggcgc agtgccagcg cgagcgcttt catcttccag
      541 ccttccggcg tgcggtacat caccttccag tagccgtcga tgaacacata atggaagcgc
      601 acctcttcag tgctttcagt ggcaatagcc tccatgaaag aacggtactc acgcgcctca
      661 ctgtccatcc ggaaaccact gtcacgtccg taataagccg tcacaccgtc caccgggttc
      721 tgaagcgtat gcccggcgga tttgtcgcaa cggggaccga ggcaacggat atctccgtgt
      781 tcaaccaggg cttcggccag ctgaccggtg gcatagtggg tatgcaggat gcgaccgttc
      841 caggtcggct ccccgtgcag gtggcagtaa acggtacgga acaccccgtc gcggcaaaaa
      901 acggaaatcg tgctgctgac tgtcatgaaa acgtctcctt ctgctgaatc aggtcgccgg
      961 gccgaccggt tgctcccccg gaggggcgaa agccccttct gccgtccact tcttcggggt
     1021 ttctccccgc agtggacctt tccgatgggg cagcgactgc cacggcacgg ggcagttgtc
     1081 cgggggcaga cgcaaaagtt tttgacgcct gcggcaaaaa gttttgtggc tggtacatct
     1141 tcccctggac ggctgccccg tgttgtggcc gcttcagccc ccggaaagtc cacagcggga
     1201 aacaggccgc aggggtggcg gcagaagggt tgactgcgcc atcaggcgca gtggccttac
     1261 acaccgtcag gtgtgcaggg agcgccgttt ccggcgcgac aggggcggcg cgaccggaac
     1321 ggtcgctgca ggcctggttt ttcagacatc ggaccgtagc ggcagcccga aaccgcgcca
     1381 gcgggttcgg cggagactgg cgggtgcgcc agcagctgcc tgtcggagtc gggccgacga
     1441 ccggatgccg ccaggcagcc gggagccgaa gggtgtcccc gcctccgggc atcagaacaa
     1501 cagaaacgac aaaacagaaa aatgcctcac accatgacgg gcaggaaggg cagcgccgca
     1561 ggcgcggccc tcctgtattt caggcaggcg taagcctgcc tgggttttcc ggcggcccgg
     1621 caggccatac ccccgccgga gcgggggagt aaatcaccga cggaacacat gaccgtcaat
     1681 aaaggtgaag gagtccagga ataaatcccg cgcatacgcc tcatagtcaa aatagagcgc
     1741 caccgtctcc ggcacgtcag ccagcaggcc ggtatcactg acgaactcaa ccgcaaaagc
     1801 ctcctcactg tcagcctcgc cccaccaggc atcgcggaag gtgtcaaaat ccgtctcacc
     1861 ggtatcatcc acccagagac gataagcctc ttcgcagcct tcatcccgtg cctggcggaa
     1921 gccttcaacc caggcccagt tgatatggca ttcggaggcc atattcaccg ggaacccctc
     1981 ataatcctga aacatcagtt caggatcggc ttcatcctgg tgaagagcac ggcaggcggc
     2041 gaaaaagtcg cgttcatcat caaacgtggt caggtcaaac cagcggccag cgatgcttcc
     2101 acagttgtat ttatgccagg ttccaacgta tacagcaggt gcaacaacag acattcccga
     2161 atcctccata gtcacatgtt ctgcgacacc gggcggtgcc gtgcctccat ctttgccggg
     2221 cctgcaggga tgcaagggcg caagggaagc cgcagcccga cgcgcacggc tgacgggagt
     2281 tggagggtga gaccgcgaaa tctgagcggg tgaacgaccg gaaaatcacg gcagacggga
     2341 aggagggacc gggtgaccgc agcggttcat cttaccttgc atcccgcccg cagaagcccg
     2401 gctacgatgg aggcacggca cagcccgcac acgacgctga ggccacataa ctgaaaaccc
     2461 acaaggccat gccccgcatg gccgggtaaa agcggatccg ccggcgactt ccttattccg
     2521 gcgagtgcag aggacacgcc gcatgacctc cagaccaccc ctgatatgaa acgatcatga
     2581 tcggcgtgtc cctgcgaaaa atatacatgc ctggttcacc ggagaatatc tgtgatatcc
     2641 acaagttttc tgtcgcacag ccagtcctgc gccgcacgca ccacgctact gtccggtttc
     2701 accgctttca gagaagatgc cagtcgtttg atcagcatca ccagttcccg tgtctgtatg
     2761 tccggcattg tctctggaag tgcagaggga ctctcatttc cgacgctcgt accatgcgct
     2821 tccagtgtgg cacggacaaa ccaggcggca tccaccagtt gctggcgaat ccaggccagc
     2881 tcggcatatc tgtcacgggc tgccattcgc tcacccgaca gcgccttgct gatatggctg
     2941 cgcaccagat tatccaggag ttcctgcgct tcttccggtg aaaagccacc tggtatctct
     3001 gtgcgcaaca gtcctttcct gtacagtagc tgcagcagat ccaatctgcg accgcactgc
     3061 aactatcagt acataacgga caatattcgc actggcggtg gtttctgtat cattcagaag
     3121 ttcctccgga tcattaccac cggtattgca gagcaacggc aacagaggtc gcatccacac
     3181 gggaacctga tattgttcgt ctgatttcat catatt
//
LOCUS       DAZFRJ010000310         3211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000310 DAZFRJ010000000
VERSION     DAZFRJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.312"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..1636
                     /gene="ydiF"
                     /locus_tag="ACHKUN_004671"
                     /pseudo
     CDS             42..1636
                     /gene="ydiF"
                     /locus_tag="ACHKUN_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            1650..2801
                     /gene="ydiO"
                     /locus_tag="ACHKUN_004672"
     CDS             1650..2801
                     /gene="ydiO"
                     /locus_tag="ACHKUN_004672"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO6662777.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(2844..>3211)
                     /locus_tag="ACHKUN_004673"
     CDS             complement(2844..>3211)
                     /locus_tag="ACHKUN_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6662778.1"
                     /translation="KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPI
                     NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKN
                     CFAEQEILSEFPQPVSLVG"
BASE COUNT          871 a          725 c          814 g          801 t
ORIGIN      
        1 atctggcgta gggaaaatca catctgaatc aggaattaac aatgaaacct gtaaaaccac
       61 ctcgtattaa tggacgagtg ccggtcctgt cggcacagga agcggtgaat tatattcccg
      121 acgaagcaac actttgtgtg ttaggcgctg gcggcggtat tctggaagcc accacgttaa
      181 ttacagctct tgctgataaa tataaacaga ctcaaacgcc acgtaattta tcgattatta
      241 gtccgacagg gcttggcgat cgcgccgacc gtggtattag tcctctggcg caagaaggtc
      301 tggtgaaatg ggcattatgt ggtcactggg gacaatcgcc gcgtatttct gaactcgcag
      361 aacaaaataa aattattgct tataactatc cacaaggtgt acttacacaa accttacgcg
      421 ccgccgcagc ccaccagcct ggtattatta gtgatattgg catcgggaca tttgtcgatc
      481 cacgccagca aggcggcaaa ctgaatgaag tcacgaaaga agacctgatt aaactggtcg
      541 agtttgataa caaagaatat ctctattaca aagcgattgc gccagatatc gccttcattc
      601 gcgctaccac ctgcgacagt gaaggctacg ccacttttga agatgaggtg atgtatctcg
      661 acgcattggt tattgcccag gcggtgcaca ataacggcgg tattgtgatg atgcaggtgc
      721 agaaaatggt taagaaagcc acgctgcatc ctaaatctgt ccgtattccg ggttatctgg
      781 tggatattgt ggtggtcgat ccggatcaaa gccaactgta tggcggtgcg ccggttaacc
      841 gttttatttc tggtgacttc acccttgatg acagcacaaa cttagcctgc ccctaaacca
      901 acgtaaatta gttgcgcggc gtgcattatt cgaaatgcgt aaaggcgcgg tggggaatgt
      961 cggcgtcggt attgctgacg gtattggcct ggtcgcccgg gaagaaggtt gtgctgatga
     1021 ctttattctg acggtagaaa caggtccgat tggcggaatt acttcacagg gtatcgcctt
     1081 tggcgcgaac gtgaataccc gcgccattct ggatatgacg tcccagtttg atttttatca
     1141 cggtggtggt ctggatgttt gttatttgag ttttgctgaa gtcgaccagc acggtaacgt
     1201 cggcgtgcat aaattcaatg gcaaaatcat gggtaccggt ggatttattg atatcagtgc
     1261 cacctcgaag aaaattattt tctgcggcac gttaactgcg ggcagtttaa aaacagaaat
     1321 tgccgatggc aaattgcata tcgtccagga aggacgggta aataaattta ttcgggaact
     1381 accggaaatt acttttagcg gaaaaatcgc gctcgaacgg ggactggatg ttcgttacat
     1441 caccgaacgt gctgtattca cgctgaaaga agacggcctg catttaatcg aaatcgcccc
     1501 tggcgtcgat ttacaaaaag atattctcga caaaatggac ttcaccccag tgatttcgcc
     1561 agaactcaaa ctgatggacg aaagattatt tatcgatgcg gcgatgggtt ttgtcctgcc
     1621 tgaagcggct cattaacagg agtataatga tggatttttc tttaactgaa gaacaagaac
     1681 tgctgctggc cagtattcgc gaactgatta cgactaactt tccggaagag tatttccgca
     1741 cctgcgatca aaacgggaca tatccgcgtg agtttatgcg ggcgctggcg gataacggta
     1801 tttccatgct tggcgtgccg gaagaatttg gtggtatccc tgcggattac gtcacccaaa
     1861 tgctggcgct gatggaagtg tcaaaatgcg gtgctccggc atttttaatt accaacggtc
     1921 aatgtattca cagtatgcgc cgtttcggtt ctgcagagca gctacgtaaa acggcagaga
     1981 gcaccctgga aacgggtgat cccgcctatg ccctggcgtt gacggaacca ggtgctggct
     2041 cagataacaa cagtgccact accacttaca cgcgtaaaaa cggcaaggtc tacatcaacg
     2101 gacaaaaaac ctttattacc ggtgcgaaag agtacccgta tatgctggtg ctggcgcgcg
     2161 atccgcaacc gaaagatccc aaaaaagcct tcaccctgtg gtgggtcgac tccagtaagc
     2221 ccggcattaa gattaacccg ctgcataaaa tcggctggca tatgctcagc acctgcgaag
     2281 tctatctcga caatgtggaa gttgaagaga gcgacatggt gggcgaagaa ggaatgggtt
     2341 tcctcaatgt gatgtacaac tttgagatgg agcgcctgat caacgccgcg cgcagcaccg
     2401 gctttgccga atgcgccttt gaagatgccg cccgctatgc caaccaacgt atcgcttttg
     2461 gtaagcccat tggtcataac cagatgatcc aggaaaaact ggcgctgatg gcgattaaga
     2521 tcgacaacat gcgcaacatg gtgctgaaag tggcatggca agccgatcag catcagtcac
     2581 tgcgcaccag cgcggcgctg gcaaaactgt attgcgcacg taccgcaatg gaagtcattg
     2641 atgatgcgat tcaaatcatg ggcggtctgg gctataccga tgaagcgcgc gtctcccgct
     2701 tctggcgtga tgtccgttgt gaacgtatcg gcggcggtac agacgaaatt atgatttacg
     2761 tagcaggtcg gcagatcctg aaagactatc agaacaaata atctgcaggc ggcgcagctt
     2821 cttaacaaac tgcgccgcca gatttatcca acaagactta ccggttgagg aaattcagat
     2881 aggatttctt gttccgcaaa acagttttta aattgtctac ggtaatcgct gggcgaacaa
     2941 ccgacatggc gcaaaaacag tttggcaaag tgatcgacat tttcataacc cacacgccag
     3001 gagatctctg cctgtgataa ttcagtatta gtgagtgacc attttgcttc cgtcatacgc
     3061 cgttgaataa catagttaat aggggaaata cgatactctt tggtaaattc atggcaaata
     3121 taactgacgc tggcacgaaa ttttttcgat aactgttcta aagtgatttt ttcgcgataa
     3181 ttattattca gatagaaaag aatatctttt a
//
LOCUS       DAZFRJ010000311         3169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000311 DAZFRJ010000000
VERSION     DAZFRJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.313"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(58..819)
                     /gene="kduD"
                     /locus_tag="ACHKUN_004674"
     CDS             complement(58..819)
                     /gene="kduD"
                     /locus_tag="ACHKUN_004674"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO6662779.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(849..1685)
                     /gene="kduI"
                     /locus_tag="ACHKUN_004675"
     CDS             complement(849..1685)
                     /gene="kduI"
                     /locus_tag="ACHKUN_004675"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO6662780.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1972..3153)
                     /gene="yqeF"
                     /locus_tag="ACHKUN_004676"
     CDS             complement(1972..3153)
                     /gene="yqeF"
                     /locus_tag="ACHKUN_004676"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO6662781.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT          833 a          881 c          713 g          742 t
ORIGIN      
        1 tctacaggta taaaaaaccc tgccatgcgg cagggtcata aaagtaagaa gaatgaatta
       61 acgcgccagc caaccgccat ccacggcaat ggtataacca ttcacataat ctgaagcgct
      121 ggaggcaagg aacactatcg gccccatcag gtcactcggc agtccccaac gaccagctgg
      181 aatgcggtcg agaatttccg cgctacgttg ttcatctgcc cgtagttgtt gagtattgtt
      241 ggtcgccatg taacccggag ctatcgcatt aacattaatg ttgtgtttag cccattcgtt
      301 cgccatcaat cgcgtcacac ccatcacgcc gctttttgat gcggtataag aaggcacacg
      361 gatcccgccc tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg
      421 cgcgataaag tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt
      481 catgacatcg tcccagtcct tttcgctgaa ctcgagagca tcttcgcggc gaatcaatcc
      541 ggcgttattc accaggatat caatatgacc aaactccgct accgcgcgat ccagcagtcc
      601 aggaatgcca tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc
      661 cgtgacctgc tcgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc
      721 cgcttgcgcc agccccaacg ccatcccctg gcccagccca gtatcacaac cagtgacgac
      781 cgcaacttta ccttcgagag aaaatgcact taaaatcata acaatacctt attcgtttat
      841 gcccacaact agcgcaaatc tttaacggcc acatggtcca tatcatcaaa gacctggttt
      901 tcaccgacca tgccccagat aaaggtataa gctttggttc cgacgccgga atgaatcgac
      961 cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc
     1021 tgccccatca tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc
     1081 atccggcgct cgtgagtgtg acacggcatg gtgttccaca agttacctgg agccagctcc
     1141 gtcagcccca tactcaattg gcaggtttcc agcacatccg ggacaaaata tttgttaatt
     1201 gtgcgacggt tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg
     1261 acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa ctttgccgga
     1321 gtggcggtat caatactggc aaagacaact tcttttgcac ctttacccac atacagggcg
     1381 tcgcggtgac cgatttcata gcattggcca tcgacagtaa tcgtaccggc accgccaata
     1441 ttgataacac ctaactcgcg acgttcaagg aaatagctta cgccgagttg tttaccaact
     1501 tccccgccaa cggaaaccgt tttagttatc ggcataatgc cgccaacaat aattcggtca
     1561 atgtggctgt aaaccatggt gtactcatcg gcgacaaata cctcttcaac caaaaattca
     1621 ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac tgtggatgct ctgtcttacg
     1681 tccacatcaa cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg
     1741 tttcgttttc tctataaagg gatgatatgc ccatgggcta gggttttcaa ttaaaaataa
     1801 aacaatgttt catttttaag ttaagaatta aaaaaagtgc agtgtcgatc acgaatatgt
     1861 catgagaatg gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca gccatcatta
     1921 ttcaacagat taatgacggc agatatttat cggtaaggag gaaatgcaga gctactcgtc
     1981 acgttcaatg gtcaatgcca cgccctggcc cccgccgata caaagcgttg ccagcccttt
     2041 gcgggcatta cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc
     2101 gcctatcggg tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca
     2161 ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag
     2221 atcgacatca gccaactgcc agcctacacg ctccaggcaa cggcgggtcg catacaccgg
     2281 cgcaattccc atcaatgccg gatctacacc aacgctggca aatgcgcgga tgcgggccag
     2341 cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc
     2401 atcgtttatg gatgatgcat tccccgcggt cacagaaccg agactatcaa atgaaggatt
     2461 taaacgggct aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac
     2521 caacgtctga ccgttacttt ggatcattac cgggacgatc tcatctttaa atcgtccggc
     2581 gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaactgacg
     2641 gctgatgcca tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa
     2701 ggcatcccac aacccatcat gcaccagact gtcaaccaac tggctattgc caagctgtgc
     2761 accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc
     2821 gatgacaata tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag
     2881 cccagaaccg caaacgtcat taatagtgat tgcagaaacg ctattaggaa gaccaccttt
     2941 aatagccgat tgccttgccg gattctgccc tgcccctgca gtcaacacct gaccaagaat
     3001 tacttcatcc accgcatatg caggaacgcc ggtacgttct attaacgctt tcacgaccag
     3061 actaccaagt tccacggcgg aatgacccgc taacgcaccg cgaaagcagc cgataggtgt
     3121 ccgtaacgcc ccgacaatca caacgtcttt catcacaacc tcgcaccaa
//
LOCUS       DAZFRJ010000312         3115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000312 DAZFRJ010000000
VERSION     DAZFRJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.314"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            124..1029
                     /locus_tag="ACHKUN_004677"
     CDS             124..1029
                     /locus_tag="ACHKUN_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6662782.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(1200..2852)
                     /locus_tag="ACHKUN_004678"
     CDS             complement(1200..2852)
                     /locus_tag="ACHKUN_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6662783.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT          911 a          756 c          658 g          790 t
ORIGIN      
        1 ttattttatg actttcagat atcgtcgaat ttataagagc acctgaattt acccataaaa
       61 tatgcggatg atcacgcata aaaatgtttt aaatcggttt ttaatgccgg actggaggga
      121 agaatgagtc gttcgcagaa tttacgtcat aacgtcataa atcaggtgat tgaagatatg
      181 gcgaggggca atattccttc tccgctgcct tcacaaagtg ggctggcaga aatgtataac
      241 atcagtcgca ctacggtacg ccacatactc cagcatttaa gcgcatgtgg cgtgctcacc
      301 ctggtgggga aaaattatgt tatcgcccgc aaacccgaac agaacgatgg atttgattgc
      361 atcacggcct cgctgaaaga gcaaaaccgt cttttcgagc aggcattttt caccatgatt
      421 aacctgcgcc agttacgcgc gggagaaagc ttttccgaac ttcagctggc gcgagcggca
      481 ggcgtcagtc cggtagtagt acgagaatat cttttgaaat tcggtcgtta taatcttatt
      541 caaagcgaaa agcgcggcca gtggagcatg aagcagttcg atcaatccta cgccgagcag
      601 ctgtttgagc tacgcgaaat gctcgaaaca cattccctgc aacactttct caaccttccc
      661 gatgacgatc cccgctggtt gcaggcgaaa accatgctcg aacgtcacag gacattgcgc
      721 gacagcattg gcagcaactt tcggatgttt tctcaattag accgtgattt tcatgccatg
      781 ctgttatccg ctgctgacaa catctttttt aatcaatcac ttgaaattat ctcggtgatc
      841 tttcattttc actatcaatg ggatgaaagc gatctcaagc agcgcaatat cattgctgtt
      901 gatgagcata tgaccattct cagcgccctg atttgccgta gcgatttaga tgcaataacg
      961 gcattgcgaa accatctgga taccgccaaa caatcaatga ttcgttccat caggcaacat
     1021 caccgataaa ttaagtacag attaaaaaca ttgattcttg gtttatatca ctatgttgta
     1081 ttaatgcagt attaatccag gcgattgttc tgcccataag gaaatacata gggaataaac
     1141 gatgctggaa gtattactct cagccatcat ttaactccct attactggta gcgataaaat
     1201 tatcggctat ccatgattaa cgtttccagc acatctaatt cactgactat ttttgccagt
     1261 gtttcatcac tgacttcttg ccgtgcacgc atctggtaaa tagccgcacg cctggcattt
     1321 aacgctgcca gattaaatgt tctttccagc ttcgcgcgct gcatatgtaa agaattatca
     1381 ttttccgaca tatcatcttc gctacgccgc atataaccga taactctggc ccctgtttcc
     1441 ataatcaact cgttgtcgag gttttctgta ctggtgcgta gcaaacgttg ctcagtcttt
     1501 tgcacactga gtatggccat tttggccata aacagatcgg cagcttcctc ttctttttgc
     1561 cggttatcgc tgaaaatgcc cacatttttc cccagtagta acggcagaat aataatgccg
     1621 caaaccagcg aaaagaggat cacacctgtg gcgagaaata ccagtaagta acgcccggga
     1681 aaaggtgtgc catccggcag caataacggg atagaaagca cccccgcaag cgtgatggct
     1741 ccgcgcaccc ccgcaaaggt cgccagcagc atatgacgcg ttttatatcc agcaaacagc
     1801 atcggatgtt tgccgggtcg tttacggcta tacagacgca tcagccaaag ccagcccagg
     1861 cgcacaacca ttaacgccag gtagatccaa atgacatcaa ggagcagcga tccggtgtct
     1921 accagtggat cgttttctgc acggatcagt gattccgaaa taatgcccgg cagttgtaag
     1981 cctaaaatca gaaacaccat gccgttaaag acatattcaa gcatcgacca gctactttta
     2041 gctttaattc tcaggcggat aggcgcgtgg tggactacgc caatgcgact ggttgttaac
     2101 cccgcggcga cagcggcaag aatgccagat aagccaaaat gttccgcaac cagataaaca
     2161 ataaacggca acagaagcat caatacaatc tgaatggaag gttcatcgtg agcatatcga
     2221 ctgatcaaca gcagcccttt gccatataac caggtaacag caatccccac ggcaataccg
     2281 cccaacgcaa cgacaaaaaa cgcaatggtt gcgccatgaa cgctgaattc catcgttccc
     2341 atcgtcactg caacagcaaa tttcaacgcc accaggccgg aagcatcatt catcaacgct
     2401 tcgccttcga gcacttcctg gatgtttttg ctgatacgcc ctttcccgac aatccccagc
     2461 aacgccacgg catcggtagg cgacaacacg gcggctaatg cgaaagcagc gatgagcggc
     2521 acattcggta agagtaaatg aatcacatag ccgacaccaa aaatagtgat tagcaccaac
     2581 acaagcgcca ggctgataat ttcccgactg tgcttgatca attctttagg ttgtattttt
     2641 gagctttcag caaacaacaa ggggggtata aacaataaga gaaaaagttc aggattgaat
     2701 tccacatgca gaccaaatat gggctgggcc agtacagcgc cagcggcaat ctggatcaaa
     2761 ggaagtggta tctgaacagg agacagacgc gtaagcacgc tggttaatga tactaccaac
     2821 gtaagaatta aaattgttaa aagtatttcc atataataaa caggcttcct cattcataaa
     2881 taatgggtat tattaattta acttcataaa aagcgccctg gttagcactt aacgttaatc
     2941 attaatcaca gcgttcactc acatgctcag gaggaaacca gtgagatgag ttttccagtt
     3001 gataacaaat tcaccatgtg aatatttact acctttaacc gttgtaacga atcatccgtt
     3061 actctttgat ctccttgccg aacaagttat ttagtgaatg atggctttct tttaa
//
LOCUS       DAZFRJ010000313         3084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000313 DAZFRJ010000000
VERSION     DAZFRJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.315"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..369
                     /locus_tag="ACHKUN_004679"
     CDS             <1..369
                     /locus_tag="ACHKUN_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6662784.1"
                     /translation="RFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLAT
                     PTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGC
                     EMRGMVANFGGFLSINKLSQ"
     gene            374..1117
                     /locus_tag="ACHKUN_004680"
     CDS             374..1117
                     /locus_tag="ACHKUN_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6662785.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDACHLAGITLPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHVSAFTGIYNDLAAASACM"
     gene            1114..1695
                     /locus_tag="ACHKUN_004681"
     CDS             1114..1695
                     /locus_tag="ACHKUN_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6662786.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            1931..>3084
                     /locus_tag="ACHKUN_004682"
     CDS             1931..>3084
                     /locus_tag="ACHKUN_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662787.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQYLASVILENLPERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQRVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTSSDVVVDDNGVG"
BASE COUNT          674 a          780 c         1007 g          623 t
ORIGIN      
        1 cgttttctgg atgcggtgaa ttttgtggcg ggcaatccgg aagcggaccc ggagcaggag
       61 ctgagcgacc gctgggtggt ggagcagatg tcagagctga cggccatgac agcctcgttt
      121 gtgctggcaa caccgacgga gacggacggg gcgctgtttc ctggtcgcat catgctggcg
      181 aacacctgta tgtgggatta ccggggagat gaatgcgggt ataacggtcc tgcggtggcg
      241 gatgagttcg acaaccccac cacggatatc cgtaaggaca gatgcagcaa gtgcatgcgc
      301 gggtgtgaga tgcgcggcat ggtggctaat tttggcggtt tcctttccat taataaactt
      361 tcgcagtaaa tccatgacag agacagaatc agcgattctg gcgcatgccc ggcggtgtgc
      421 gcctgcggag tcttgcggct tcgtgataag cacgccggag ggtgaccggt accagccgtg
      481 cgtgaatatc tctgcagagc ctgaggcata ttttcgtatt gccccggaag actggctgcg
      541 ggcagagatg cagggggaga ttgtggcact ggtccacagc catcccggtg gcctgccctg
      601 gctgagcgag gccgaccggc ggctgcagat aaaaagtgcc ctgccctggt ggctggtctg
      661 ccggggggac attcataaat tccgctgtgt gccgcacctg accgggcgtc gttttgaaca
      721 cggggtgacg gactgttaca cgctgttcag ggatgcctgc catctggcgg ggataacgct
      781 gccggatttt caccgcgagg atgagtggtg gcgcaatggt cagaacctgt atctggacaa
      841 tatggaggcc actggttttt accgggtgcc cctgtcctcg gcacaggcag gcgatatcct
      901 gctgtgctgc tttggcgcat cggtacccaa ccatgctgcc atttactgcg gcaacggtga
      961 actgcttcac catctgcctg aacaactgag taaacgggag aggtattccg aaaaatggca
     1021 acgacgaacg cattctgtct ggcgtcaccg ccactggcac gtatctgcct tcacggggat
     1081 ttacaacgat ttggccgccg cctcagcctg tatgtgaaca cggcagcgga agccatccgt
     1141 gccctgtcga tgcagatgcc gggattccgc cgtcagatga acgaaggctg gtaccagata
     1201 cgtattcgcg gtgaggacac ggcaccggag gcggtgtacg cccgtcttca cgaacagctg
     1261 ggtgagggaa cggtcatcca cattgtgccg cgactggccg gggccggaaa gggtggactg
     1321 cagattgtgc tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcaatg
     1381 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
     1441 ctgggggcca gcatgatact gggtggtgtg gctcagatgc tggccccgaa ggctaaagta
     1501 ccggagtaca aaagcacgga taacggcaga cagaacacgt acttttcctc gctggataac
     1561 atgattgcac aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggctcacgg
     1621 cgaatctccc aggacatcag tacccgtgat gaaggcggtg acgggaaggt ggtggttatc
     1681 gggcggcagg cataaaagcg aaaaaatccc gcagtgaccg aaggctgcgg gaacagaaaa
     1741 tgaagattaa ccacagggag ttttgttttt attggcccga aaaaactgta acgcccggga
     1801 atgatatctg ccacgggggc gtacagaaaa tgtgaagata ttcagaattt ttattcagtc
     1861 atgatacagg catcctccgg gatgcctgtt gtttttgtgc gtaacagtta tcacagtaaa
     1921 gggtgagaca atgggcaaag gtggcggcaa ggcgcacacg ccggttgagg caaaggacaa
     1981 tcttaagtcc acgcagatga tgagcgtgat tgatgcgatt ggtgaagggc cgattgaagg
     2041 tccggtgaag gggctgcaga gtatcctggt gaacaaaacc ccgctgacgg acacggacgg
     2101 taatcctgtg atacatggtg tgacagcggt ctggcgcgcc ggggagcagg agcagacacc
     2161 acctgaaggc tttgagtcct ccggggcgga aaccgcactg ggcgtggaag tgacgaaagc
     2221 aaagccggtg acgcgcacca ttacgtccgc gaacattgac cgcctgcggg tcaccttcgg
     2281 ggtgcagtca ctgttggaga ccacctcaaa gggcgaccgt aatccctctt ctgtccgact
     2341 gctgattcag ttgcagcgta acggtaactg ggtgacggaa aaggatgtca ccattaacgg
     2401 caagaccacc tcacagtacc tggcgtcggt gattctggag aatctgcctg agcggccctt
     2461 taacatccgg atggtccggg agacggcgga cagcaccacg gaccagctgc agaacagaac
     2521 gctgtggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggccat
     2581 tgtggggctg caggtggatg cggagcagtt cggcggtcag cagatgacgg tgaactacca
     2641 tatccgcggt cgcatcatcc aggtgccgtc aaactacgac ccggaaaaac gcacttacag
     2701 cggtatctgg gacggcagtc tgaaaccggc atacagcaac aacccggcct ggtgtctgtg
     2761 ggacatgctg acccacccgc gctacgggat ggggaaacgc ctgggggccg cggacgtgga
     2821 caaatgggcg ctgtatgcca tcgggcagta ctgtgaccag cgtgttccgg acggcttcgg
     2881 agggacagag ccgcggatga cgtttaatgc gtacctgtca cagcagcgta aggcgtggga
     2941 cgttctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg gccagacgct
     3001 gacgttcgtt caggaccgtc cgtcggatgt ggtgtggccg tacaccagca gcgatgtggt
     3061 ggtggatgat aacggcgtgg ggtt
//
LOCUS       DAZFRJ010000314         3042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000314 DAZFRJ010000000
VERSION     DAZFRJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.316"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(208..810)
                     /locus_tag="ACHKUN_004683"
     CDS             complement(208..810)
                     /locus_tag="ACHKUN_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662788.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(897..1103)
                     /locus_tag="ACHKUN_004684"
     CDS             complement(897..1103)
                     /locus_tag="ACHKUN_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6662789.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1855..2004
                     /locus_tag="ACHKUN_004685"
     CDS             1855..2004
                     /locus_tag="ACHKUN_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO6662790.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2816..>3042
                     /locus_tag="ACHKUN_004686"
     CDS             2816..>3042
                     /locus_tag="ACHKUN_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6662791.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          784 a          730 c          816 g          712 t
ORIGIN      
        1 tgcgtggtgg tgatgaatca acaggcatat aactgatacc cgccggtcag aagttccagc
       61 ctgcgacttt cctgtctgcg tgcccgcagt ccggcgttca ggtgcagcgt gtgacagaac
      121 gcacggagag cgaaacggca ggcagacagg ccgttcagtc agccctggca ccctgcccgg
      181 ataccagctt ccctgaaaca actggcatca ggtgcgctta tcgtcaaccg ttgtgcggta
      241 tgccaggagc acgtactgat ccatcagatc cgtcagtgct gcgctctgct tctgacggtt
      301 gatgatccat tcctccagct ctttttctga tttccgtttc agattctccg tccagtcgtc
      361 aaggcagttt ccggtcatta aatcaagcca gcaaagccag accagtttga ggcgcaactc
      421 cgtgccacca aactgacgga agaggctgat cagtttattt gccagcgaac gctgcctcag
      481 acgttcacat tcagacagga gaatgccttc cagcatcgga tgatgaacca gcagatgttc
      541 actggtgcat aaattcacta tcagatcatc atccaggggc ggcgtggtcc gtttgtccgg
      601 accataaatc agccggtgca gactctgggg tacgatggca atctccggtt tttcaccatt
      661 gaatgacacc cacccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      721 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcag
      781 ctcgtaacgg gtctgatact gtggtttcat tttcatattc ctcacggggg ataacaggca
      841 gcagaacgcc gccggcaggc cggagactgc gtctgctgca gatgaacggt taagaattac
      901 ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcgctttt cagccatcgg
      961 gagctgcggc ccattttgat gggcgcagga aagtcaccat ccttgatgag tttgtaaaac
     1021 cacttatcgg tcaggccggt cagttgagtg ataaatgcca tgtcgaccat ctggtcatcc
     1081 atcagtgaaa ctggggtgat catgagaatg ttcctccgga tgttgttaaa ccagccggtg
     1141 tggtcatgaa aacaacgcac caccttcacc ggcataacaa ccggggagaa acgtaaaaaa
     1201 taatcgggaa tgtgaccgcc aggagataaa cggggaggtt acggtggctg agccgtaccg
     1261 gtatgccggg tggttgttac gaaacgatgc ttccgcacgc gcgcgcgata aagaagggat
     1321 gattttcgtg aggaagaggg gaaaactggc gttctgataa ttgcagagtt atttacgggg
     1381 cggttacgat ggggcagacc gtgtccggtg ggcaggcgac aggtatcacc gatgggataa
     1441 gactgtctga ttggtattat aaatatataa gagagatcaa acacatgaga tcaaatacat
     1501 gagttcatat atgtgaaata aacacggtga gtgtgatcag tgggcatgat gagaacgcag
     1561 tagcaacaga ctgcgctatc cctgatagct ggcgtataac ctgcgtatat aaaacgtacc
     1621 agcaacgcac gtgaacagag tgcattaagc atccgtcgtt gacaccgacc tggtgagtga
     1681 cagtctgaga gaccggttaa gtggtggtac gggagtaatg agtttgggga gggtaccaga
     1741 atgtcagtct ggtacggtat gctgacagta tcacgttaat tgcagcataa agtgatgaga
     1801 ggctaatgga agttaaaacc agagcagttc cgtcgttgtt catcccggga gacactggaa
     1861 aaagtgattt cccacacgcg ttataagctt actccagcgg agatggaagc cttcaactct
     1921 gcggtcgatc accgactggc agaactgaca atgaacaagc tttacgatcg tgttcccgct
     1981 tcagtctgga aatatgtcat ctgagcgttc ggtaatcaca actgcatcgc gctaggtgtt
     2041 atccgctttg tgattcttat tggctcttct caggttatta aaagctgaat atgttcctgt
     2101 tgtcgggaac caggataata ccgggagcgt taagcggtat agcgacaaca aagaattcga
     2161 aatcaaaagg gaatggtgcc ggaggcgtgg gcggtaatag gctgattttc aggtattacc
     2221 ggcttacaac gtgtggttga tattgagggg ctgtgagttt cgcgtatttg tcagaaaccc
     2281 agatgagttt tcctgtatgg gacgaaataa ggcagcggca atacctggaa aatttaatat
     2341 ggtatgaagg agaacctgtc ggttacgctt aatatacacg gtacactgtc agctcagggg
     2401 atacatgaac cgttatctca ccagctacac agtatacaca cactgacgct gacagtagtc
     2461 agcaggacca caataatcag cgccagcagt atggtaacga tacggtctgt attccggtag
     2521 tagccgaagg ggtcgacaga accacaatac gggcaacaca ctgtattatt agtaatcgcg
     2581 tttccacagt ccctgcactt cttttgcgta tcaccataac tccctcctca cagccttacc
     2641 cgtaaccggt tttttacatt aaaaaacaac gctgaaaatt caaccgtcag tccggagacg
     2701 accgttcggg ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca cttcagggac
     2761 tctgcgcatt ttttacgacg tggctattcc gtatgaacca tacggagatt taaccatgac
     2821 ctacaaatac aaccccttct ggcagcaacg tattcgtgag acggtgcggc acgcactgga
     2881 tgttcatccc cgtctgacgg cattgcgggt tgatctgcgt ctcccggatg taccggcagc
     2941 aacggacgca gctgtgatat cccgcttcat caatgccctg aaagcccgaa tcgacgctta
     3001 ccagaaacgt aagcatcggg aaggtaaacg cgtgcatccc ac
//
LOCUS       DAZFRJ010000315         3034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000315 DAZFRJ010000000
VERSION     DAZFRJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.317"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..180
                     /locus_tag="ACHKUN_004687"
     CDS             <1..180
                     /locus_tag="ACHKUN_004687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662792.1"
                     /translation="QSIQDFSPTSVQLFPFFINVKKRLCFGIKRKTQEDMSVTVLLSP
                     HPLPWIVNAYHPYLS"
     gene            155..1366
                     /gene="bluF"
                     /locus_tag="ACHKUN_004688"
     CDS             155..1366
                     /gene="bluF"
                     /locus_tag="ACHKUN_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO6662793.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1571..2302
                     /gene="bluR"
                     /locus_tag="ACHKUN_004689"
     CDS             1571..2302
                     /gene="bluR"
                     /locus_tag="ACHKUN_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO6662794.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2523..2927
                     /locus_tag="ACHKUN_004690"
     CDS             2523..2927
                     /locus_tag="ACHKUN_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO6662795.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          895 a          637 c          648 g          854 t
ORIGIN      
        1 caatctatac aagatttttc gccaactagt gttcaacttt ttccattttt tatcaatgta
       61 aagaaacggt tatgttttgg tattaaaaga aagacacaag aagatatgtc tgttaccgtc
      121 ttactctcgc ctcacccatt accctggatt gttaatgctt accaccctta tttatcgtag
      181 ccatatacgt gacgacgaac ctgtcaaaaa aatcgaagaa atggtttcga tagcaaatcg
      241 caggaacatg cagtctgacg taacagggat cttactgttt aatggttctc attttttcca
      301 gcttctggaa ggtccggaag aacaggttaa aatgatatat cgggctatat gccaggatcc
      361 acggcactat aatattgttg agctgctgtg cgattacgcg cctgctcgcc gttttggcaa
      421 agcgggaatg gaattatttg atttgcgcct gcacgagcga gatgacgttt tacaggccgt
      481 attcgacaaa ggcacatcaa aatttcagct aacttatgat gacagagcgc tacaattttt
      541 tcgtactttt gtccttgcaa ccgaacaatc aacctatttc gagatccctg ccgaagactc
      601 ctggcttttt atcgctgacg gatctgataa agaacttgat tcctgtgccc tttcaccaac
      661 tataaacgac cactttgcct ttcatcctat tgtcgatccc ttatcgcggc ggataatcgc
      721 ttttgaagcc attgtgcaaa aaaatgaaga tagcccatca gccatagcgg ttgggcagcg
      781 taaagacggg gaaatctaca cagcggatct caaaagtaag gcgcttgcat tcacgatggc
      841 acacgcactt gagctcggtg ataaaatgat ttcaatcaat ctattaccta tgaccctggt
      901 taacgaacct gacgcagtct cttttttact taatgaaata aaggccaatg ctctggtgcc
      961 tgaacaaatc atcgttgaat ttactgaaag tgaagtcata tctcggtttg atgagtttgc
     1021 cgaagcgatt aaatcgctaa aggctgctgg tatcagtgta gcaattgatc attttggcgc
     1081 aggttttgct ggtttgttac tcctgtcacg cttccagcct gacagaatta aaatcagtca
     1141 ggaattgatt accaatgttc ataaaagtgg gccacggcag gcaattattc aggcgatcat
     1201 aaaatgctgt acatcacttg aaattcaagt cagtgctatg ggcgtggcaa caccagaaga
     1261 gtggatgtgg cttgaatccg caggaattga gatgtttcag ggagatctgt ttgctaaagc
     1321 taaattgaat ggtatccctt ccatagcgtg gccagagaaa aaataatttt cagcacattc
     1381 tttcacatga tttcagtaaa tcatcgtgaa aatgaatacg ttacgtttaa atttaatcca
     1441 cattaaaaat aatgtttcat ccaatttagc tgaaatctta tttatacttt gtacaatttt
     1501 agcgtatatt gtacaaagta ttattgggtc gtgtacaggc gacggagatt tgtgaccgca
     1561 aggaggaatt gtggcttatt acagcattgg tgatgttgct gaacgttgcg ggattaatcc
     1621 tgtcactctc cgggcctggc aacgccgcta cggtttgtta aaaccacagc gcagtgaagg
     1681 cggacaccga ctctttgatg aagaagacat acaacgcatc gaagagatca agcgttggat
     1741 aagtaatggc gtccctgtag gcaaagttaa agcattactg gaaaccacca gccaggatac
     1801 ggaagatgac tggagccgcc tgcaagaaga gatgatgtca attcttcgca tggctaatcc
     1861 tgccaaacta cgcgcgagaa ttatttcact gggtcgagag tacccagtag atcaattgat
     1921 taatcatgtt taccttcctg ttcgccagcg tctcgtgctt gatcacaaca cctcccgcat
     1981 tatgagcagt atgtttgacg gcgcattaat tgaatacgca gcaacctcgc ttttcgaaat
     2041 gcgccgtaag cccggtaaag aagccattct gatggcgtgg aatgttgaag agagagcacg
     2101 attgtggctg gaagcatggc gtttatcatt gtcaggatgg cacatttctg ttcttgctga
     2161 tcccattgaa tcgccgcgcc cggaactgtt cccgacgcaa acattgattg tctggacagg
     2221 catggcacca acgagaaggc aaaacgaact tttacaacat tggggtgagc aaggttataa
     2281 agtcatcttt catgccccct aatttcacct ttatcccgcc ccttagacag aatgttataa
     2341 tctgaaccca caacagcgtt tgtaccggag ctggtccgtt gcaaaatgct caccgggaga
     2401 attcagatat tccctctatc acactctgta catatcacag ccaaaggaca gaccagattg
     2461 aagtggtaaa accgatcgtg cacactacgc agtctcacaa ctaacaagag gttattatcc
     2521 tgatggatca ggtcgttgtt tttcaaaaga tgtttgagca agtgagaaaa gagcaaaact
     2581 tctcctggtt ttattcagaa ttaaaacatc accgtattgc acattacatt tattatctgg
     2641 ctacggataa catcagaatt attactcacg atgacacggt tttgttatta agaggaacca
     2701 ggaacctgtt aaaagttagt acgaccaaga accctgctaa aataaaagag gccgcattgc
     2761 ttcatatttg cggaaaatct acatttcggg aatactgttc aacactagca ggcgcgggcg
     2821 ttttccggtg ggttactgat gttaatcata acaaacgcag ttactacgcc attgataata
     2881 cgcttttata tattgaagat gtagaaaata ataaaccatt aatctagcta aagttggatg
     2941 cttaagaaat gcttcataat tcagtaaggc attagcataa tgggaataaa agtacagaga
     3001 ctatccctat ggatgataaa tactgtcctt ttat
//
LOCUS       DAZFRJ010000316         3029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000316 DAZFRJ010000000
VERSION     DAZFRJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.318"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(28..1887)
                     /gene="gss"
                     /locus_tag="ACHKUN_004691"
     CDS             complement(28..1887)
                     /gene="gss"
                     /locus_tag="ACHKUN_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO6662796.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2092..2958
                     /gene="yghU"
                     /locus_tag="ACHKUN_004692"
     CDS             2092..2958
                     /gene="yghU"
                     /locus_tag="ACHKUN_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO6662797.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          712 a          815 c          768 g          734 t
ORIGIN      
        1 atcagtacgg ggttgctaca acatgtatta ctttttcacc acaattaacg gttcaatatc
       61 actctctttt ttgatgacca gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc
      121 gccaacggtg aaggtacata cctgaatgta tttaccgtcc actttcggca aacaccacag
      181 ttgctgatag atgtttttct gctcggcaaa tttaccgctg gttttgtcca gcacctcttc
      241 atgatggctg accaggttga tattgctacc acagcgaccg gcaatcggtt tcactgcgta
      301 acctgttttc accaattcat cattaacagt gaaatcggta tccagcaggt aacgatggtg
      361 cgggaacagc gaccagagga tcggcagaat cgctttgttg ccggggatca ccgtccacag
      421 cggctcaaag actagcactt ccgggcgcag caatacgtcg ataagacgca cttcgttttg
      481 cggatgaccg gtacggattg gcaccgcagc aaactcacgg tcgctaactt cacgaatctg
      541 atcaaacgcg gtttcccacg cccaggtttt ccacacgcag ttaaccagcc gtccttcccc
      601 atcaatcagt tgcccggcag catcccagcc cagttcatcc agcccgcgca agatacgcgt
      661 ttcaaagccc tcctggtgca gcgcctgctc cataaactgc gcgtgatagt tttcctcgat
      721 atctttgtcc tgcatgatat ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc
      781 cagttcgtta atcagccctt ccgccggatt gaagccgttg cctttatagc cctgctccgc
      841 ccaacgttcg aggatcaagc ccgcttcggt atgacatgag gcagagtcgg cgttgtattc
      901 gtaaaccttc aggccacgct catccatgca gaaatccata cgaccagtga tcatatggtg
      961 acggcgacgc tgccaggaga gacgcaaacg tggccagagg attttcggga tgtcgaacag
     1021 cgccagcagg ttgtcatctt tcagcacctt gtcggttgcg tgaagataca tcaggtgcag
     1081 ctcgttggtg gctttaatta gctcctgctc ggcactctcg gtaatggtgt agtaatgata
     1141 aggatcctga ttgatcacct gaccgttggc ctgcacatag gcgttttgca gcggatcttt
     1201 ttcatccagc catttaccgt caaactggcc tttgttttcc aggcgcgctc cgctgatttt
     1261 cagcagctcg cctgcaattt ccggctgcgg taagctgtat tcagtatctt ccgtctggat
     1321 catccagccc agaatggtgg tgtcatcaaa agtgtctttc agggtatagc agccgttttc
     1381 gaccaccatc tccagctcac gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac
     1441 gttctgttcc gcaatacgga ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc
     1501 agtgtcttta aattcaccgc ctttatccca gataagaagc gcacccgcga ccggcgcacg
     1561 cggcgagccg ttaggaaatg cctgcaatgg caggatgttg tcattaacca cttctcgcag
     1621 gaagcgcagc gagaaaatct cccacgccat acccacgtca gtaaagacca caccgtaatt
     1681 cagaaagaga aaacggcgag caaattcaac gcattgccac ttgtggccca tatattcgtc
     1741 gtcgatatag ctacggaata cggcgtcatc ttcgtattcc tgcggatcga gagaactgta
     1801 atctgaagag tagattgcta ccccacctgg ggcgtagccc aataatgtcc cgaacggggc
     1861 atcctggctg gtcgttcctt tgctcatcac tttaccttta acaatacaac ctaagcagtt
     1921 ggcgtaattg ttgtgctctg attacctgct cagcgacatt aaccggacag aggtcagact
     1981 aatcatacac ctcatcgcag cggctggcgc aaagggttaa aaaattcaca ttctgtacag
     2041 caagtgacct gctacactgc ttcaacacta ccactcagaa ggcaactcac tatgacagac
     2101 aatacttatc agcccgcgaa agtctggacg tgggataaat ccgctggcgg cgcgttcgcc
     2161 aatatcaatc gcccggtttc tggtccgacg catgaaaaaa cgctgcccgt tggcaaacac
     2221 ccattgcaac tttattcgct gggaacgccg aacggtcaga aagtaacgat tatgcttgag
     2281 gagctgctgg cgctgggcgt tactggtgca gagtacgacg cctggctgat tcgtattggc
     2341 gatggcgatc aattctccag cggctttgtc gaagtgaacc caaactcgaa gatcccggcg
     2401 ctgcgcgatc atacgcataa tccgccgatc cgcgtgtttg aatctggttc gatcctgctt
     2461 tatctggcgg agaaatttgg ctacttcctg ccgcaggatt tggcaaagcg tactgaaacg
     2521 atgaactggc tgttctggtt acagggcgcg gcaccgttcc tcggcggtgg ttttggtcac
     2581 ttttaccatt acgcgccgat gaaaattgag tacgccatca accgctttac catggaagcc
     2641 aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc ataagtttat tgcgggcgat
     2701 gagtacacca ttgcggatat ggcgatttgg ccgtggtttg gcaatgtggt gttaggcggc
     2761 gtgtatgatg ccgcagagtt tcttgatgcg ggcagttata agcatgtaca acgctgggcg
     2821 aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta ttgttaaccg caccaacgga
     2881 ccgctgaatg agcagttgca tgagcgccat gacgccagtg atttcgagac gaatacggaa
     2941 gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa gccatccagg ccggataagg
     3001 cgttcacgcc gcatccggca atcgtgcac
//
LOCUS       DAZFRJ010000317         3021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000317 DAZFRJ010000000
VERSION     DAZFRJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.319"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1002)
                     /locus_tag="ACHKUN_004693"
     CDS             complement(<1..1002)
                     /locus_tag="ACHKUN_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6662798.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     "
     gene            complement(1004..1282)
                     /locus_tag="ACHKUN_004694"
     CDS             complement(1004..1282)
                     /locus_tag="ACHKUN_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662799.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            complement(1349..1600)
                     /gene="rem"
                     /locus_tag="ACHKUN_004695"
     CDS             complement(1349..1600)
                     /gene="rem"
                     /locus_tag="ACHKUN_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO6662800.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            complement(1817..1972)
                     /gene="hokD"
                     /locus_tag="ACHKUN_004696"
     CDS             complement(1817..1972)
                     /gene="hokD"
                     /locus_tag="ACHKUN_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6662801.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(2044..2331)
                     /gene="relE"
                     /locus_tag="ACHKUN_004697"
     CDS             complement(2044..2331)
                     /gene="relE"
                     /locus_tag="ACHKUN_004697"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO6662802.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(2331..2570)
                     /gene="relB"
                     /locus_tag="ACHKUN_004698"
     CDS             complement(2331..2570)
                     /gene="relB"
                     /locus_tag="ACHKUN_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO6662803.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            2595..2900
                     /gene="ydfV"
                     /locus_tag="ACHKUN_004699"
     CDS             2595..2900
                     /gene="ydfV"
                     /locus_tag="ACHKUN_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO6662804.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
BASE COUNT          743 a          797 c          703 g          778 t
ORIGIN      
        1 cagctccagt tgggagccat acttatcttc aaatgccact gtatccgtat gcagctcgtt
       61 gtgatgcttt ctgcacaaag gcaacacaaa gagatcatgt gcttttgttc ccattccgcc
      121 ctgcccgtga ccaatcagat gatgcggatc gtcggctggc ataccgcagc aagcacacgg
      181 ctgtgtctta acccaacgtg tgtatttctc cttaacccag cggcgacgtt taggcagctt
      241 catgaaagat tccggagact ctggatcaac ggtgatgctt accaccgtct tttcctgtgg
      301 tgatttttgt tgctggtggg cgtaaggcaa cggtgcaaga ttttttgtgc gttgtttcaa
      361 tatgctggtg gcggtctgct ctcccggtac gatgtcgctt tcgcggtaca ccgagcagat
      421 tttttccgct ggtaatccca gcgaacgacg cgatacagcc tcaggtagtg catccaccac
      481 ctgattgcag accgcccacc aggataattc agccaaagat aattcccgct cctgcgtacc
      541 gcttattgcg tgacggatga cgtcaatcac ccatgctgtc agattttgtt gagcaagcag
      601 ctccagtgat tccgatgtct ggtcacgcag ttggttgtcg cagtgccagc acaacaccat
      661 tgcgccggta ccataacggt gaatgactgt ttcagtgtga tggtaatcac cattaggcca
      721 ctggcaggat gtaacatgac gtaatagcca gtcggacaat gcgccaacgc cgccagcagc
      781 acgaatcacc cgttcgttac taaaaaacgg cagcaatgtt ttgtcttccg ccagcggctg
      841 gcgaacggca ggaacgactc cggatggcag attacgcatg ctttttggtt ccggttccac
      901 cagcactcga ggattatgaa atatctgtat ggattcacgg cccggcttaa ggaccaccag
      961 cccaagctca ggcaccagaa caggtctaag taatacccgc acgttacctc cagatccgtt
     1021 gctggaaagt gcgggacgca cgtggtgggc gttcggaata aggcagcctg acagagatta
     1081 tccagtgccg atagtcgaga ctgagagctt tcttaacctc gaacccgcgc ctgcggtaag
     1141 aatgaatcag ccattcggcc tgttctgcag tgcatggagg gtgctggaac cattcagact
     1201 tgaatgcgtg agaataccgc ccgtgcgtgc aggcaagaac gggcgaatta tcagaattgt
     1261 aatattttgc gttgcgtgcc atcggttttc tccggtggca cggtgttact cagcgggagt
     1321 tcagccccgc gcaagattgt agatgagttt attctcctga aaaagcagaa aagccagctt
     1381 ttattccgat ctctttcaat gcctgtaatg aagtgacaaa ctcaccttcg cgcaagataa
     1441 atccgtccgt gacccgagca tccacaaaat taattaacgc agccccattc tttcgcaaac
     1501 acataatgcg gtaatgacta acaagatttc cattttcaac gcacacagca tagaggccat
     1561 cttcacaaaa aattttacgc agttcttcga tgttcatcat cagaatcctt ccggataatt
     1621 agctctcccc tttaagggac catccctctt atccctgcgc gctacttaag tatttttgat
     1681 tctattccgg caccgtccag aacttcaaac gcgttgaaaa taaaaacaaa aacccgccga
     1741 agcgggttaa gtgcgggtgc gttgaggatg cctgccacat cagaggtggc gagggatttc
     1801 tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg acctccgtct
     1861 ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc gtcactatga
     1921 cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc atagcctgct
     1981 tctcctgtca acgcaaagca gaagtgtcac cttcggtgcg aaacagagat gtcatgcttt
     2041 ggttcagaga atgcgtttga ccgcctcgct atatacttcc gagcgttctc ttttcccaac
     2101 agaaatcacg aaaacgacaa ctttctcgtc tataacctgg tatacaaggc gatagcctga
     2161 agaccggagc ttaatcttgt aacaatcagg cataccacgg agcttgtttg cttcaatccg
     2221 gggtgactca agtacttcaa ccagcttctt tttcaactgt tcacgtaccg tcgagcccag
     2281 ctttcgccat tcctttagtg cccgctcgtc aaaatccaga aaatacgcca tcagagttca
     2341 tccagcgtca cacgtactgg cttaggatta cgaagccgtt ctttcactat ctccacaagt
     2401 tcagcatctt catcactcag gagtgtctgt ttgaacggca agcgttcatt gtcagcgata
     2461 tactcgagca tgagacgaag cgcttcagaa ggagttacac ccattttttc aagcgcggcg
     2521 taagaacgcg ctttaagttc atcgtcaata cgcaggttaa tgctacccat gtcttacacc
     2581 tcttgtaatt acaaatgtca ttacaagtat cgcactacaa catgcttagg gcaagtcacg
     2641 aaggaagtca gaaagtagtc gtaagaacgg tgatcactgt ccgctttgtg ccaggagcag
     2701 ccattgctaa gtccatcctg tattgtgcag gtcagctcgt ttttaaagag tccggccatc
     2761 atcttactgg tacagacacc atatactttg tgacggtcag gctacatatg cacaactcaa
     2821 cttattcatc tattttttgc tttagcatgt cagtgttgct ttctcgtcgg cgggtgagcg
     2881 gtgacctgac ctgtcgataa aggaacgtag cacgttttat gcaacacccg catgcggcag
     2941 aaaattattg ccgaacgttt acccctgtca acaagcttta ctttctgagg cgcgccagcc
     3001 cgcgaggaaa acaatctgaa c
//
LOCUS       DAZFRJ010000318         3006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000318 DAZFRJ010000000
VERSION     DAZFRJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.320"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="ACHKUN_004700"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="ACHKUN_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO6662805.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VEESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="ACHKUN_004701"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="ACHKUN_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO6662806.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="ACHKUN_004702"
     CDS             902..1549
                     /locus_tag="ACHKUN_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO6662807.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(1591..2121)
                     /locus_tag="ACHKUN_004703"
     CDS             complement(1591..2121)
                     /locus_tag="ACHKUN_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6662808.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHKUN_004704"
     CDS             complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHKUN_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO6662809.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          870 a          593 c          666 g          877 t
ORIGIN      
        1 tataacctca gtgtcaaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga ggaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac ggatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatag caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tcagttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acattcgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggt actttcccca gacagcgttt aatattaaag ggggagaaaa caatgacatg
     1921 gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat tattcattaa
     1981 tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt cttcgttctg
     2041 tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga acatcgtatc
     2101 cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcagtt
     2161 tgccaaaggt aattaacagg acagggctta taataataat agttagctta ctgtatatta
     2221 attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg gtggtgttat
     2281 ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc accccagccc
     2341 ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt attaaccact
     2401 tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt atacgggact
     2461 ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac aaagtgatta
     2521 cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc ctgatagttg
     2581 gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc taacaacgta
     2641 tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg ttgaccaaca
     2701 tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg ttggttattg
     2761 cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag gctgaggatg
     2821 agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa agccataata
     2881 gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt aacgtaaacc
     2941 tgtctgatgt ttatatccct tttgtttagt tcaattccat tgattttggg gcgtggcatg
     3001 agtatc
//
LOCUS       DAZFRJ010000319         2972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000319 DAZFRJ010000000
VERSION     DAZFRJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.321"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..81
                     /locus_tag="ACHKUN_004705"
     tRNA            6..81
                     /locus_tag="ACHKUN_004705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHKUN_004706"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHKUN_004706"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(300..1058)
                     /gene="yhdZ"
                     /locus_tag="ACHKUN_004707"
     CDS             complement(300..1058)
                     /gene="yhdZ"
                     /locus_tag="ACHKUN_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO6662810.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1066..2169)
                     /gene="yhdY"
                     /locus_tag="ACHKUN_004708"
     CDS             complement(1066..2169)
                     /gene="yhdY"
                     /locus_tag="ACHKUN_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6662811.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2179..>2972)
                     /locus_tag="ACHKUN_004709"
     CDS             complement(2179..>2972)
                     /locus_tag="ACHKUN_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6662812.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          808 a          789 c          723 g          652 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcaa aaaggccatc cgaaaggatg gcctttttgc ttttcgaact aacattcaat
      301 taatggatta cctgcgataa aaatgccctc gtacgctctg atttaggatg cgtaaaaaat
      361 tcatcaggtg cagcttgctc cactatttcc ccacgatcca taaaaatcac ccggtcagcg
      421 acggttcgcg caaaccccat ctcatgcgtt acacacaaca ttgtcatacc cgattgcgcc
      481 agcccaatca tcgtatccag cacctctttc accatctccg gatcgagcgc cgacgttggc
      541 tcatcaaaca acataatttt cggcttcata cacagcgaac gcgcaatggc aacgcgttgc
      601 tgctgaccac cagaaatttg tccgggaaac ttatgcgcat gttcggcaat tctcacccgc
      661 tcaaggtaat gcatcgccag agcttcagcc tctttctttg gcatcttgcg tacccaaatc
      721 ggtgccaggg tacagttctg tagaacggtc agatgaggga agagattgaa atgctgaaag
      781 accattccca cttcctgccg gacgcgctca atattgcgga tatcttcatt aagttcgatg
      841 ccatctacca cgatccgtcc ctgttgatgt tcttccagat gattaataca acgaatggtt
      901 gtcgatttac cggaacctga agggccacac agaacgatcc gttctcccgg ttgcacggtt
      961 aaatttatat ttttcaaaac atggaattgt ccataccatt tattgacgtt ttccagcgta
     1021 atcatcgcgt tagcaggttg cagtaaaatt tggctcatag tgtcctcaat gcggtgtacg
     1081 cccggtgtta aaacgttttt ccagatgctg gctatagcgc gacatgctga aacagaagat
     1141 ccagtagatc agcgcggcga aaacataccc ttccgtcgac atacccaacc aggtgggatc
     1201 aacggttgcc tgctgaacgc tactgaaaag atcgaacaac ccgataatga tcaccaggct
     1261 ggtatctttg aagagtgcga tgatggtatt taccagccca ggaattacca gcttcaacgc
     1321 ctgtggcaga ataaccagcc cctgagtttt ccagtaaccc aacgccagcg actctgccgc
     1381 ttcatactgc cctttaggca gtgcctgtaa tccgcctcgc acgacttccg caacatatgc
     1441 tgactgaaac aggatcacgc caaccagcgc gcggatcagt ttgtcgatac tggtgccttc
     1501 tgccataaac aacggcagca tgaccgaaga cataaacaat acggtaatca gcggtacgcc
     1561 gcgccagaac tcgataaaaa tgaccgataa gatacgcaca atcggcatat gggagcggcg
     1621 acctaacgcc agtaagatcc cccacggtag cgcccccgca atcccaacgg atgcaataat
     1681 taaagttagc gtcagcccgc cccattgccg ggtttcaacc cgctccagac caaaaaaacc
     1741 gccatacatc agccaccaga caatcagtgg gtaaatcacc gcccagaccg caatatagcg
     1801 accgcgatgc gggagtattt tccagaacat tggtgcgatt gacacaaggc caatcagtaa
     1861 tgccaggtta attcgccagc gttggtcgtg tgggtaaagt ccatacataa actgaccaaa
     1921 tcgttcgtgg atgaagaccc aacaggcacc ggcttttgtg cagtcggcac gcgttgagcc
     1981 aacccaatta gcctgcaaaa atgcccagtt cagcaacggt gggatcagtt cccacatcaa
     2041 ccatatgcag ccaatagtca gcaggctatt gctccagctg gagaacagat tttttcgcac
     2101 ccacaccatc gcgcggcttg agttatggct cgctgggcgc gagggctgag acagcaatac
     2161 ttttgtcatc atgactcctt agcgttcaac gatcgcgatg cggcggttat agatattcat
     2221 cagcagcgag atagtcaggc tgataatcag atagaccgac atggtcatgg ctatcgtctc
     2281 gatggcttgc cccgtctggt tcagcacggt gccagcaaac agcgaaacca tatcgggata
     2341 gccaatagcg gcggcaagag aggagttttt gacgatgttg agatactggc tggttaacgg
     2401 cggaataatc actcgcagtg cctggggaat aatgacctgg cgtagcgtaa ccgggttggg
     2461 taatcccagt gaccgagccg cttcatgttg accataaggc actgcctgga tcccagcgcg
     2521 gataatctcg gcgataaatg cagatgtata aaccgaaagt gccagcgtta aggctgccag
     2581 ttcaggaatt aaaaccatcc cgccgcggaa gttaaagcct cgtagggctg ggacatccca
     2641 gtgtagtgct gcgccaaaaa gccattgcgc cagtaaaggc aaaccaatga tcaacactgc
     2701 ggcgatcggc caggtgcggc gcagttgtcc ggtctttatc tggtatgttt tattaaagcg
     2761 gaatagccca acagaaagga ttatagccat aacaacagcc aggataaacg caataaatcc
     2821 gtctcccagc tgcggtgacg gaatataaag cccacgattg ctcaaaaagg ccagatcaaa
     2881 cgcgctcaca gcctggcgtg gtccgggcaa attgcgcaac acggcaaagt accagaagaa
     2941 gatttgcagc agcgggggaa tattacggaa ga
//
LOCUS       DAZFRJ010000320         2969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000320 DAZFRJ010000000
VERSION     DAZFRJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.322"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(106..182)
                     /locus_tag="ACHKUN_004710"
     tRNA            complement(106..182)
                     /locus_tag="ACHKUN_004710"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Asp,seq:gtc)
     gene            complement(315..1046)
                     /gene="dnaQ"
                     /locus_tag="ACHKUN_004711"
     CDS             complement(315..1046)
                     /gene="dnaQ"
                     /locus_tag="ACHKUN_004711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO6662813.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1111..1578
                     /gene="rnhA"
                     /locus_tag="ACHKUN_004712"
     CDS             1111..1578
                     /gene="rnhA"
                     /locus_tag="ACHKUN_004712"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO6662814.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1575..2297)
                     /locus_tag="ACHKUN_004713"
     CDS             complement(1575..2297)
                     /locus_tag="ACHKUN_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6662815.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2331..>2969
                     /gene="gloB"
                     /locus_tag="ACHKUN_004714"
     CDS             2331..>2969
                     /gene="gloB"
                     /locus_tag="ACHKUN_004714"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO6662816.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQI"
BASE COUNT          810 a          717 c          694 g          748 t
ORIGIN      
        1 agcataacaa aaccatagac cgaagaaata gcgggattta aatgcaaaaa aaaccgcaaa
       61 attaaaaatc ttgcggttct ctgaactcat tttcatgagt gaatttggcg gaacggacgg
      121 gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
      181 ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc cttcgtcaac
      241 ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagccgct atttttagcg
      301 ccttttacag gtatttatgc ccgccagagg cagcttccgc ccttcttctc caccagatca
      361 agacgggctt catgagctgc aagctcttca tctgtcgcaa aaacaacgcg taacttactt
      421 gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgctgtgt ctctccttcc
      481 atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc
      541 tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc gtagcgggca
      601 cataacgcat cgaggctgtt gcgcttacca ggaaacattt tcctcgccac cgcaaggcta
      661 tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg
      721 tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc gccgcggata
      781 tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa aaattcatcg
      841 gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca
      901 tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc
      961 ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg
     1021 atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt cgttttacag
     1081 ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat
     1141 ggttcgtgtc tgggtaatcc tggacctggg ggttacggcg ctattttacg ctatcgcgga
     1201 cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat ggagttgatg
     1261 gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac
     1321 agccagtatg tccgccaagg tatcacccag tggatccata actggaaaaa acgtggctgg
     1381 aaaacagcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgca
     1441 ttggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac
     1501 gaacgctgtg atgaactggc ccgtgctgcg gcaatgaatc ccacactgga agatacaggc
     1561 tatcaagtcg aagtttaagc ctgtggttta cgatattgcc gggtggctcc aaccgcctgg
     1621 cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg aatagtccgt
     1681 ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgtgcatt caatagtttt
     1741 ccaccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgcagcac ttcaaaattc
     1801 aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctgtt atagggcgag
     1861 gtttttcgca ataccggcac aagtttgcgt aatcccatta aactaatggg attgaagcca
     1921 ctaatcacca gccagccatc atcaatcaat acccgatcgg tttcacgcaa taaacgatgc
     1981 ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca
     2041 aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc agaaacattc
     2101 acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta
     2161 agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc cagcgccttg
     2221 cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac agtttgaggg
     2281 actcttgccg gtttcatcac aaccttccgt ttcacactaa gaggtaatct atgaatctta
     2341 acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct
     2401 gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc gccaataact
     2461 ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc ggcgtaaaag
     2521 aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca caagataagg
     2581 gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat gaatttagtg
     2641 taattgccac accgggtcac actttaggac atatctgtta cttcagtaaa ccttatctat
     2701 tttgcggcga cacgctgttt tctggtgggt gtggtagatt gtttgaaggg acagcatcac
     2761 aaatgtatca atcacttaat aagttaagtg cgttacctga cgatacattg gtatgttgtg
     2821 ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg cacgatttgt
     2881 ccataaatga ttattatcgt aaagttaagg agttacgggc aaaaaatcaa ataacactac
     2941 ccgtaattct gaaaaatgag cggcaaatt
//
LOCUS       DAZFRJ010000321         2934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000321 DAZFRJ010000000
VERSION     DAZFRJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.323"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(125..2386)
                     /gene="katE"
                     /locus_tag="ACHKUN_004715"
     CDS             complement(125..2386)
                     /gene="katE"
                     /locus_tag="ACHKUN_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO6662817.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2569..2832
                     /gene="cedA"
                     /locus_tag="ACHKUN_004716"
     CDS             2569..2832
                     /gene="cedA"
                     /locus_tag="ACHKUN_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO6662818.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT          705 a          720 c          761 g          748 t
ORIGIN      
        1 aataaactgc aatacattga atttatacga ctttgtaggc cggataaggc gttcacgccg
       61 catccggcat aaacaaagcg cactatgtaa atcatttgag gcggcgcaat tgcgccgcct
      121 cccatcaggc aggaattttg tcaatcttag gaatgcgtga ccacacgcgg tgtgctgcca
      181 tcagcgttag cagttcatcc ataaaactac cgtcagcgct gtcagcttcc acaatccctt
      241 cttcaccctg gtcagcgacc ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg
      301 caatcggttt aaggtgtttg taggcttcca tcaggtagta gttggcatcg ccgttgtcag
      361 cgatatccgc gatattgccg caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac
      421 cggcaaaggt agcggctata ggcaacaccg taccgtcatc cgcagtcact tcacccattc
      481 gggagtagag cagtttggca tgaacgcctt tggccttcag ggccttgaga atggccagaa
      541 ggtctgccga tctcacttca tcattaagta aaatcgctac cacgcgacct ttcacatcac
      601 cgtcaggaat ggcgtacaaa cttaaggatg gatccttttt cagaccgttg acgtccggag
      661 gtggggtgat attcagctgg tcgtcagtca gttcgatacc gagatttttc gccaccgcct
      721 gggccagagt gagatcaata tgcgccagct ggtcaacaac gcgctcacga atatacggac
      781 gaacgacttt gcttaactca aaactgaaac catcgacaat atggcgctgc tcgaatggcg
      841 tctgacttag ccagaacaga cgcggatggg aataatattc gccaaacgat ggactgcgct
      901 cacgaacttt atttccttcc acgcgttcct gatatgattc aaaaccgccg cgtttcggtc
      961 ccggcggtgt ttcgcgcggc cagttatcgt tgatcgagtt cggttcgtaa ttcgccgggt
     1021 tagtgtcgat ccccatacga tgcatgccat cacgctggaa gttgtggtaa gggcaggtcg
     1081 gacggttaat cggaatctca tggaaattcg gcccaccaag acgactgatt tgtgtatcgg
     1141 tataggagaa caaacgtccc tgcaacagcg gatcgttggt gaagtccaga ccaggaacaa
     1201 tatgacctgg atggaatgct gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat
     1261 tgagcaccat tttgccgaca cgctgaacgg gcaccagttc ttccgggatc agtttggttg
     1321 gatcgagaag atcgaagtcg aacttgaatt catcttcttc aggaatcaac tggaagccca
     1381 gttcgtattc cggaaaatcg cctgcttcaa tggcttccca caactcgcgg cggtggaagt
     1441 ccgggtcacg tccggtgagt ttttgtgctt catcccaaac gagtgaggct ttacctgcca
     1501 gtggtttcca gtggaaacgt acaaacgttg ccttcccttc ggcattaatc aggcggaagg
     1561 tgtgaatacc gaagccttcc atggtgcggt aactgcgggg gatgccgcga tccgacatcg
     1621 cccacatcac gttgtgcaga gtttcaggtt gcagagaaac ataatcccag aaagtatcgt
     1681 gggcactttg cccttgtgga attgcccagt gcggttctgg ttttaccgca tgaacaaaat
     1741 cggggaattt atgcgcatcc tggataaaga agattggcgt gttattgcca acgaggtcaa
     1801 aaataccctc ttcagtatag aacttggtgg caaaaccacg gatatcacgc acggtatcgg
     1861 cagagccagc gccaccctga acggtagaga acctaacaaa tactggggtg attttgttcg
     1921 gatctgagag gaaatccgct ttggtgatat cgcttaagct tttatatggc tggaaataac
     1981 cgtgagctgc tgatccgcgt gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt
     2041 gggtgatttt ctcgcgcaga ataaaatctt ccagcagcgt tggaccacgg ttaccggcac
     2101 gcagtgagtt ttgatcatcg gcgatgcgca cgccctgatt agtggtcagc gcataatttt
     2161 cactgccttt gcgtacgtct tccagagaat taagtttttc gttacgtgta tcaggggctt
     2221 tcaggctccc aggggcggta ggttgtgcac ctggcggtgt tggttcagcc gctggacgat
     2281 gagatccgtc ctcaggtgcc agtgagtcca tccccggttt cgcttcgctg gaatcgtgta
     2341 gtggtgactg gtgctgatgt gggttctttt cgttatgttg cgacattgaa ctcgtctcct
     2401 taatttatta ctgaaagggc cgctgttttg gtcaaaaaac cctctaacta tagaccatca
     2461 gccagatccc gcttcggaga ctattctgga aacggtgtta tgaaatacac gcaaagtggg
     2521 acgtacaggg gctaaatcgg ctaaactaca gtttttctga tttttaatat gcgtttagtg
     2581 aagccagtaa tgaagaaacc gctccgccag caaaaccgcc agattattag ctatgtccca
     2641 cgcacggaac ccgcgccgcc agaacatgcg ataaagatgg attcttttcg tgatgtctgg
     2701 atgctgcgtg gcaaatatgt tgcgtttgta ctgatgggag agtcatttct gcgctcaccg
     2761 gcgtttactg tgcctgaatc cgcccagcgt tgggcaaatc agatccgcca ggaaggggaa
     2821 gtgactgagt aaagattacg tacctcccgg ttttgccggg agttacatga cctgaaattc
     2881 tgtatttcat tgctacattc attcaaacca ccagtaacaa aaagctgcct ttga
//
LOCUS       DAZFRJ010000322         2907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000322 DAZFRJ010000000
VERSION     DAZFRJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.324"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..100
                     /locus_tag="ACHKUN_004717"
     CDS             <1..100
                     /locus_tag="ACHKUN_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO6662819.1"
                     /translation="GKIIEPVIELYNDVVWASFNTLHNKINPYKEN"
     gene            102..365
                     /locus_tag="ACHKUN_004718"
     CDS             102..365
                     /locus_tag="ACHKUN_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662820.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            376..501
                     /locus_tag="ACHKUN_004719"
                     /pseudo
     CDS             376..501
                     /locus_tag="ACHKUN_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1057..1245
                     /locus_tag="ACHKUN_004720"
     CDS             1057..1245
                     /locus_tag="ACHKUN_004720"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6662821.1"
                     /translation="MPDTKTAVLVARDFGRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WIPSHWMPLPEPPQEVRQ"
     gene            1361..1465
                     /locus_tag="ACHKUN_004721"
     CDS             1361..1465
                     /locus_tag="ACHKUN_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662822.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            1712..1867
                     /gene="hokD"
                     /locus_tag="ACHKUN_004722"
     CDS             1712..1867
                     /gene="hokD"
                     /locus_tag="ACHKUN_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6662823.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2035..2313
                     /locus_tag="ACHKUN_004723"
     CDS             2035..2313
                     /locus_tag="ACHKUN_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662824.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2315..>2907
                     /locus_tag="ACHKUN_004724"
     CDS             2315..>2907
                     /locus_tag="ACHKUN_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662825.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEHLAQQNLAAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV"
BASE COUNT          759 a          688 c          776 g          684 t
ORIGIN      
        1 cggaaaaata attgagccag ttattgaatt gtataacgac gtggtatggg cgtcattcaa
       61 cacactgcac aataaaatta atccgtataa ggaaaactga tatggcaact ttgacaaaaa
      121 aagaacgggc atggttgaac gaattacagg aagttcttga tcgctgtcca tcaccgaaaa
      181 aaattggctt ttacaccatt ggcgataaaa gcatttacct gtatgaccta cgccgcatgg
      241 atgaaatcat ggaggctctt gataatcgtt cgtcgatgga ttggtgtgtt gctgttcatg
      301 atatgaatgc agggtttgat gaaaagattt tgttcccctc atcagttgaa agcactgcgg
      361 gttaaggagt aacacatgac cactattacc aaagaacgta ttgaattgtt cattaaaaat
      421 ccgcttgaaa acgggcttac ccgtggtgaa caaatggaac tggcacggat tgcgctggca
      481 tcgctggaag cagagccggt ggcaaagatt atagctcatt acccattagg ggttgacgta
      541 ggcaaacaaa aatttgtaca ggccattaga gagcttcctg actttggcgg atatctattt
      601 gccgcccctc cagcgccgat agtgccggaa gaaatgtatt ggcaggatgc gccagttgaa
      661 ggcagcagca aagcggctgc atacgctaca ggctggaacg attgccgcga agccatgttt
      721 cagtccggaa actttcggga aaataaagat tcgtcaacca ataattttcg gaaaatcccg
      781 gaagcgtcaa ccagctctcc ggtaactccg gctcttctgc ctggtggttt caccattgag
      841 gaggcgaagg aattacatga agacctggca cgcagccaca taagcaaggc cttaagtggc
      901 gaaaagatga aaaagaaaga tcgcgatgct gatttgcgct ggattcatgg cgttatagtt
      961 caggcagcgt ggtttgtaaa agcatcactg gagcagaatg cactatcggg caactatccg
     1021 gtaactccgg atagttggat aagctgtagt gagcgaatgc cggataccaa aacagccgtt
     1081 cttgttgcca gggattttgg caggaaaggt gactggcgaa tgaaatgggc gacttacatc
     1141 ccggggcatc ctgacgctaa tgatgggtgg ataatacctg gtgcgtcgtg gataccatca
     1201 cactggatgc ctctaccaga accgccgcag gaggtgcgcc aatgaactgg cctgaagcat
     1261 ttgcaattac aggcgttgct atggctatcg cttttttagt atatgttatt tgtcggtgag
     1321 ggtaaaaacg ttcgccggga ttaacaccaa aggagggaat atgtcggatg atatatcact
     1381 ggcaatggaa ggtgcgctgg ctgttgttgc tgttgtgggc gtttactgcc tggttgtgtt
     1441 tttgatggat cgactaggga actgaattca ttacgatatg ggaattccca tatcgggtaa
     1501 aaacggtttg cgggaaaagg agagttaagt agaattgctg cgggtgcttg aggctatctg
     1561 tctcaggcat gaacaccaaa aggcagatag agaaaagccc cagttaacat tacgcgtcct
     1621 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     1681 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     1741 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     1801 cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga
     1861 ggagtaagag acccggcggg ggagaaatcc ctcgccaccg ctgatgtgtc aggcatcctc
     1921 aacgcacccg cacttaaccc gcttcggcgg gttttgtttt ttctggtcgt tctggtttac
     1981 aatccatccg tcagcctgaa caactggcac ctgctgcgcc agcagagaaa acagatggcg
     2041 cacgatacca aattttacaa ttcggataac tctgccgccc ctgccagcag gcacgggcgg
     2101 cgttcccgca ctttcaaatc tgactggttc cagcaccccc catgcactga agaacaggcc
     2161 gagtggctaa ttcagtgcta ccgcagacac ggatacgaga ttaagaaagc cctcagcctc
     2221 gattatcgtc actggataat ctccgtcagg cttccttact ccgaacgacc accgcgtccg
     2281 tcccgcacat tccagcaacg catctggagg taacgtgcgg gtattacttc gacctgttct
     2341 ggtaccggaa ctcgggctgg tgatcgttaa gccgggccgt gaatccatgc cggtattcca
     2401 caatacccgg gtactggtgg agccggaacc gaaaagcatg cgtaatctgc cgtccggggt
     2461 cgttcctgcc gttcgccagc cgctggcgga ggataaatca ttactgccat ttttcagcga
     2521 cgaacgagtg attcgtgctg ctggtggcgc tggcgcattg tctgactggt tactgcgcca
     2581 tgttaaatcc tgccagtggc cacacggtga ttatcaccac agtgaaaccg tcattcaccg
     2641 ttatggtacc ggcgcaatgg tgttgtgctg gcactgcgac aaccagctgc gcgaccagac
     2701 ctccgaatca ctggaacatc tggctcaaca aaacctggca gcctggatga ttgacgtcat
     2761 acgccatgca atgaatggct cgcaggagcg tgaattatct ctggctgaat tatcctggtg
     2821 ggcagtctgc aatcaggtgg tggacgcatt acctgaggca gtatcgcgtc gctctctggg
     2881 attaccggcg gaaaaaatcc gctccgt
//
LOCUS       DAZFRJ010000323         2868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000323 DAZFRJ010000000
VERSION     DAZFRJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.325"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(500..874)
                     /locus_tag="ACHKUN_004725"
     CDS             complement(500..874)
                     /locus_tag="ACHKUN_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662826.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(897..1115)
                     /gene="ydfC"
                     /locus_tag="ACHKUN_004726"
     CDS             complement(897..1115)
                     /gene="ydfC"
                     /locus_tag="ACHKUN_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6662827.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(1145..1273)
                     /gene="ydfB"
                     /locus_tag="ACHKUN_004727"
     CDS             complement(1145..1273)
                     /gene="ydfB"
                     /locus_tag="ACHKUN_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO6662828.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(1275..1430)
                     /locus_tag="ACHKUN_004728"
     CDS             complement(1275..1430)
                     /locus_tag="ACHKUN_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6662829.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1703..2419)
                     /locus_tag="ACHKUN_004729"
     CDS             complement(1703..2419)
                     /locus_tag="ACHKUN_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6662830.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            2469..2684
                     /locus_tag="ACHKUN_004730"
     CDS             2469..2684
                     /locus_tag="ACHKUN_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6662831.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            2681..>2868
                     /locus_tag="ACHKUN_004731"
     CDS             2681..>2868
                     /locus_tag="ACHKUN_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6662832.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWL"
BASE COUNT          734 a          652 c          640 g          842 t
ORIGIN      
        1 gtgcctggtg cctccaggtg acgttaacca gttaacaatt aacgccggat aaagagaatc
       61 cacccataac actgtttttg gttttaactg ttccgcgtgc gctcagccgc attcaccaca
      121 tcacaaaatt cactttaaaa agggcggcag agcagtcacg gagtaaaact gataccgcca
      181 aaagtcacca gaaaattgat aacagagggc gttgcagcgg agttgtcact taagcgtatg
      241 gtcaacctga caacccggtg tcctcaacgg gggaaggaat aaccccgcca tacttaccgc
      301 cgcgccattt cgcggattgc cacaaccgga agcgcacgat cgaattaaat ttaacgacga
      361 cctatacaga gagactaact tctccgggcg ctttcgtgtt atgccctgac ttttcaggga
      421 tatatccttt tcagtaaact gtcagtgccg gattcttatc cgtgtccggc gcacgcactc
      481 tacctcacct gtgaataaat taatgattaa ttgatatttt gttgtttgat tcaactttcc
      541 catcggatgt gtgatgcttt aaatcacagg aattaatact gcttgctgta aaatgatttt
      601 caaggggagc tattcgaatc cctttctttt tcattaacaa gccaaatcct ttattaatga
      661 tgtccattaa ttccagaaag tatttttcat gtaaatcctg gttatcagag agctgcttct
      721 cttcgtacag cccgataaag gctcggcgta cgttaccaga tatattgtcg atggtttctt
      781 tttctacggt actcaggtca agagtcgcca gttgagagcg aaccacattc gctgccattt
      841 cctggaatgg cattggtaaa tctttaaatt ccattattag cctcgttggt tagctattaa
      901 cgcgggtatg taatcattct ggcaatgctt aatgccgctg ctttttccag cctggtgata
      961 tcctgctcca gagcggacag attttcagcc tgcttagccc tggcttcatt ggcccatttc
     1021 aggtcctgcg cagccttaat tttctggtgc atccactcat aaagttcatc atcggtatag
     1081 tctggcgcga tgatgacggg ttctcgtttc tgcatactga ttcctcgcgg tgctgtttcg
     1141 cttatcagcc gttagatttt gccgaactgg aaagcgcctg tttaaactca ctgaagctga
     1201 gggcttcttc gccttcggca aggccttcga agtattcttc gtaagccttt tccatgattg
     1261 tgttgaaatc catatcactc acctgagttt ctttccagcc agcgacgggc accattttcg
     1321 gttttaaacg ttttgcttct ggtatacgtc atcgcggtga acgtaccgtc ctggttgggg
     1381 aacacgccac ataccagaga ttcgctgttg ccaagatcga tagtatccat gctgacctca
     1441 tttcccctta acgccggggt agcggaacaa aaacctgctg catagttatt aaagttgaac
     1501 cctgccgtca tgttcttacg cctcgggctg gctacttaac ccctgaccac tgccgggtaa
     1561 ctcgaagtat ttccctgcgt tctgtggggc ggggtgggtt ggtatgttgt taaggtaaca
     1621 agagttacct ttcgagtcaa tacaatgttg caaaaggtac atttgagggc ataaaaaacc
     1681 cgcaatgaat gcgggttctg actcagtcta agtattgatg tatttgtgaa actttacctt
     1741 taatggtgta accaccattc agttcgatgg gtttgtaaag cggattcagt gacaacagat
     1801 agatgtttgg tccgtcaatc gcaacttttt ttagtgttac gtttggcgtt ccttccaatt
     1861 ggattaagat tatttttccc accagttctc taatgttact tgagcatggt gtgatcagca
     1921 cggtagatcc gtcggggatg gttgggaggc cgttagagtt tgtcatcgca tctccctcaa
     1981 catgcaataa aaaagagttt tcagcggttt ttgtcatgac atcaacccag ttcttaatac
     2041 caggaatctt ggttactgga caactcatat cccaataacc agcctgttcc cacgttaaaa
     2101 cgggcaaccg ggcgatgttg tcactaatgt aagggtactg attcagacgc agatcatcgg
     2161 ttttatcgtg accgtccttt ccataaagaa tccattcagg agatttggaa agcaattttg
     2221 acagtagata caaattctca ccgtcaggtt ttgaagagcc attttcccat tttgttacgg
     2281 atacacgaga tatgccgatt gctttcgcaa cctgctgttg ggttaatcca acgtcttttc
     2341 gacgattccg aatacgttcg ctgatagtgt ttttcatgta accaatgtta ctaccaagtg
     2401 atgttgctat ggttgacatt gttatgtaac tattgttacc cttctgctcg aaataacagg
     2461 agagttttat gttcaaagat gatgttctgc gctatttcaa aaaaaagcga ctagtagctg
     2521 aggctcttgg aatttcacat gtggctgttg tgcggtggaa agcagttatt cccaaacttc
     2581 gcgcaatgga actggatgaa attactaacg gtgaattgaa atacaaccca gaactttaca
     2641 agaagcagga tagcacctcg aacgaaggaa agaatgattc atgaaaatca agcatgaaca
     2701 catccgcatg gcgatgaatg tttgggcgca tccggacggc gaaaaagtac cggctgcgaa
     2761 aattaccaaa gcgtatttcg agctgggaat gacgttcccg gaactgtatg acgacagcca
     2821 tccggaagcc ctggcccgta atacccagaa aattttccgt tggctgga
//
LOCUS       DAZFRJ010000324         2860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000324 DAZFRJ010000000
VERSION     DAZFRJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.326"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            261..986
                     /locus_tag="ACHKUN_004732"
     CDS             261..986
                     /locus_tag="ACHKUN_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662833.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            944..1621
                     /gene="bglJ"
                     /locus_tag="ACHKUN_004733"
     CDS             944..1621
                     /gene="bglJ"
                     /locus_tag="ACHKUN_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO6662834.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1659..2447)
                     /gene="fhuF"
                     /locus_tag="ACHKUN_004734"
     CDS             complement(1659..2447)
                     /gene="fhuF"
                     /locus_tag="ACHKUN_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO6662835.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2588..2824
                     /locus_tag="ACHKUN_004735"
     CDS             2588..2824
                     /locus_tag="ACHKUN_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO6662836.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
BASE COUNT          759 a          617 c          711 g          773 t
ORIGIN      
        1 tgatttctgt ttttcattaa gtattcctaa caacttaatg tagatatttt aaatgtctcc
       61 aggctatttc accaggttat catttgatgc taaccttctg acacatgcag tggagttgtt
      121 gtgcagcagg agtatgctga tatgaaagtg aatgctaagg ataatttatt cgcttaatct
      181 attaatttgc tggaaacatt taaggaatga atatcctgaa taaaccatca taatttttag
      241 acagtggatg tggagggaat atgttgccag gatgctgcaa aaatggaatt gttatcagta
      301 aaatacctgt tatgcaagca gggttaaaag aggtcatgag gactcacttc cctgaatatg
      361 aaataatatc cagcgcctct gcggaggacc ttaccttatt acaattacgc cgttccggat
      421 tagtcattgc tgatttagcc ggtgaaagtg aagatccacg ttctgtttgt gaacattatt
      481 attctttaat ctcacaatat cgggaaattc actgggtttt catggtgtca cgctcctggt
      541 attcccaggc agtagaactg ctcatgtgcc ctacggcgac gttattgtct gatgttgaac
      601 ccattgagaa tctggtcaag accgtacgtt ccggcaatac gcacgcagag cgtatcagcg
      661 ccatgctgac ctctccggca atgactgaaa ctcatgattt tagctatcgc tccgtcattc
      721 tcactctttc agagcgcaag gtactgcggc tattaggtaa aggatggggc atcaaccaga
      781 tagcttcatt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac agtgcgatgc
      841 gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc gcgcagggtg
      901 caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac acagccgaat
      961 taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agtagtatcg gtattgagag
     1021 tttattcaga aagtttgcgg gtaaccctta taagctccat acctatacca gtcaggagtc
     1081 atttcaggat gccatgacgc ggatctcgtt tgcggcggtc attttttctt tttctgccat
     1141 gagaagtgag cgcagagagg gattatcttg cctgactgaa ctggcgatta agcttccgcg
     1201 tacccggcgt ttagttattg cggatgatga tattgaagct cggctgattg gttcattgtc
     1261 gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt ttcatcagga
     1321 actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca atcagtggta
     1381 cattaaccaa agccggacgc taagcccgac ggagagagaa atattgcgct ttatgtcgcg
     1441 tggttactca atgacacaaa ttgccgagca gcttaaacgt aatatcaaaa cgatccgtgc
     1501 acataaattt aatgtgatgt cgaaactggg cgtcagttct gatgcagggt tgttggaggc
     1561 cgcagatatt ctgttatgta tgcggcattg cgaagcaagt aatgtgttgc atccctatta
     1621 atccgcatga tgccgggttt acttccccgg cagtgctttc atttcagcgt acaatcgcca
     1681 cattgctgca catccggtaa gcgataacgc tggcagcaag tgcggcgcac cagcaggccg
     1741 tcgcgcagta ccacggtacg ccagagtgga ttatcttcac cggtagtaag cgttttctca
     1801 aaaaagaggg catggcgcag cgattcaacc gtcgcctcgc cgagcagctg tttcatctca
     1861 gtcagatacc agttgatcaa ataaccggta ttactccaga taagtttgcc gttgatctct
     1921 cccgtcgctt ctagtgcctg cacaaccgga accagcgcct ggctgattaa cgtttccatt
     1981 cgctgctgcg gcgaatgtgg tgttgcgttt ttatcttcac acacatcgac ccagaaacag
     2041 gcggcacggc cggtttcgtg aaactcaacg tggaaatgtt ccggcgacac atctaacgct
     2101 ttttcctgcg tcagcagcgc cagcattaat ggtggcacca tcaggccgat ataccattgc
     2161 gcccatagtg agatcagcgg cttgttctcg cggatcatcg ttggttggtt gcgatagata
     2221 tgatcggaat agaccgccag tagagaactt agcgcattcg gtgatgacca ttgcgccagc
     2281 gtcatggcgc gaagcggggc gggttcatcc agacggataa actccagcaa atgttcacga
     2341 tgttcggcga tcgtcgcccg tacggcttgt gcaagcccag catcctgcgg ctggagatgc
     2401 gttcgccaga tgacatcttc atagagcggt gcggaacgat aggccataat cgggatagta
     2461 atctaaatga taatgattgc taatcctagc gataggttta cccgatagca agggatttct
     2521 ctggcttgca aatgataaaa attatcatat gatattggtt atcattatca atgaaagaga
     2581 tgaaaccatg ttgcaacgta cgctgggcag tggctgggga gtgttgctgc cgggattgct
     2641 gattgcaggg ctgatgtatg cggatttatc gccggatcaa tggcggattg tcattctgat
     2701 gggattgata ttgacgccgg tgatgctgta tcacaaacag ttacggcatt acgttttgct
     2761 accatcgtgc ctggcgctta ttgctggcat catgctgatg ctaatgaatt tgaatcaggg
     2821 atgaaaaatc aaggaagaaa caagaaagga agtaaagata
//
LOCUS       DAZFRJ010000325         2843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000325 DAZFRJ010000000
VERSION     DAZFRJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.327"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKUN_004736"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKUN_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO6662837.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..2833
                     /gene="safA"
                     /locus_tag="ACHKUN_004737"
     CDS             2636..2833
                     /gene="safA"
                     /locus_tag="ACHKUN_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO6662838.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          757 a          665 c          687 g          734 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     2821 tttgcaagac tgaaaataaa cag
//
LOCUS       DAZFRJ010000326         2831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000326 DAZFRJ010000000
VERSION     DAZFRJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.328"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(158..1636)
                     /locus_tag="ACHKUN_004738"
     CDS             complement(158..1636)
                     /locus_tag="ACHKUN_004738"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO6662839.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     gene            complement(1663..>2831)
                     /locus_tag="ACHKUN_004739"
     CDS             complement(1663..>2831)
                     /locus_tag="ACHKUN_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO6662840.1"
                     /translation="KFMGFSNTEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSA
                     MIITGLLGLLMATYPPLWVMLCIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGW
                     MEGLRGVGVMSLAVFTMWVFSRFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNN
                     LRSANNEEKQSFQLSDILAVLRISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSL
                     VAASYMGIVINKIFRALCGPLGGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSN
                     PQSVAMGIGLILLLGFTCYASRGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVY
                     PIIGHWQDTLPAAEAYRNMWLMGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          738 a          749 c          644 g          700 t
ORIGIN      
        1 cgaactagtt aaagttttta ttatgttccc tgcatcagcg gggataaacc ggacttgtta
       61 tcatttttta agttgtcgaa cttatgttcc ccgcgtcagc ggggataacc atttaggtat
      121 gttcaggccg ggcagttttc cgcccggcct acctcattca cgcattcgac cagctctgga
      181 tcgaatccac attccccaat tccccatgca tcacttcata cgattttttc aggatcacat
      241 cagtatgttt ggttaggtct ttgaaaatgc ctttattcat cgcgtcgtag cgtttggcat
      301 tgctctcaat cggtataaag atatctttca cgcgaaccat cttatcaaca gccgttgcgt
      361 aatccgggta tagccccaga cctaccgctg tattaatcgc tgctcccaga cttgcacaac
      421 cgttaatggc gttacggcgt gccggaaggt tgaacacgtc ggcaaaaatt tgcataaaca
      481 gatcgctgtt cgaaccgccg ccagtaatga tcacatgctt cgcaaagtga ttcatttcat
      541 tacacatatt gtcgtaattg ttcttcagcg ttagcgccac gctttccaat atcgaacgat
      601 atatccatgc gtaatccatg ctggaatcaa agccgatcat aatcccgcgt ttgtacggtt
      661 cccacggatt ggtcagccag tccagcaccg tcatcagccc attacagcca ggtggcacac
      721 tagaggcttt cttgttgagt aaatcttccg gtgaaagatc ctgcgccctg gcatcctgaa
      781 ttaacgactc gcctaacatg tcacgcagcc agctcaccgt ccacatacct ttgcgaatac
      841 cgtaaccttc atacagcaat gtttgcggaa tagaagacat aatcggccag tacgccaccg
      901 gatctttcgg cagtgctttg ccgttcatca tcaatgcgat gtaagtgcca agagaaatta
      961 ccgcagtttc atcatccagt aatccggccc ccagagcttc taccggttta tcactggtgg
     1021 tacaaacaac cggcagtcct gccgggaaat gtgtcgccag tgcggcttgt ggtgtgatat
     1081 gtccgaggac ggtgccaggc atttgcacat caaacagcat atgacggggg atattaaact
     1141 tatccattac cgcagcatct tcgctccatg cccagctctt ataatccacc ggccactgac
     1201 caaaatagtt ggcgatattg tctttaaact cgccggttaa gcgatgcgtc agataacccg
     1261 aaaaagaggt gacatacgcc acgtcaggat tcgtatgttc gtaagggcgt gtaacgcgtg
     1321 catcctgcca gctaatcaac ggcgcagcag gcgtgccatc ggctttcaat aacgcacggc
     1381 agcaacggat ggagcccagg ccgatgccaa caatatcttc tttattcccg gcaaactggc
     1441 tcatcaaatc gtgacctgca aaacataatg atgcccataa atcgtcgtca ggatgttctg
     1501 cagtatcggc ataaggcgtg tgcatcggct gtaataagcc tttaccttcg caaaccacac
     1561 taccttccag atcgtacatc accacttttg tgctctgact tccgccatca atccctatga
     1621 tgtatttttt cgacatgatt tttctcctgt aattttcctg gcttacttgc tgctagccat
     1681 tgcgggggcg ctatcagcag tacgaatttt ttggaacagc aaaaaggtaa agacaatcac
     1741 catggcaagc gccgccattc ccatcagcca catattgcgg taggcttctg ctgcgggcag
     1801 agtgtcttgc cagtggccga taatcgggta aacgaagaca tccggcagga atccaatcac
     1861 cgaacaaata cctaccgtgg tgcccataat gtaagacggt gttctcactt cgccagggca
     1921 ggcccagtac agcccgcgtg aggcgtaaca ggtgaatccc agcagtaaaa tcaggccaat
     1981 ccccatcgcg accgattgcg ggttagagtt cgtgacgagc agggcagtta acgccagcag
     2041 gccgagtacg gaaaggattt ggatcacgcg ggtaggggat ttcactttac tgtaggtggt
     2101 gataatgccg ccaagtgggc cgcacagcgc gcggaagatt ttgttgatca caatccccat
     2161 gtagctcgct gccaccagcg acatgccgta catttcggtc agatagttgg tggagtaact
     2221 cagaatggcg tagattgtga agacaccaaa aatcaccatg ctgcaatacc aggtggtgct
     2281 gatacgcaaa acggcaagga tgtcgctaag ctggaatgac tgtttttctt cgttattggc
     2341 actgcgcagg ttgttgttat cgctaacaaa aaaccagcac agaatcccca acaagatgta
     2401 aaccacactg tagatgataa tgactgtttt caggctggcg ctgtcatccg gtgcaaagcg
     2461 agaaaagacc cacatggtaa acaccgccag cgacattaca ccgacgccgc gcagcccttc
     2521 catccagccc ataattttcc cttgctcact atgatcgccc agcaacgagg cggctttaat
     2581 cgacaccgac cacagcatta aaatcgtcgt tatcgcaaag gcgacctgaa tacagagcat
     2641 tacccacagc ggtggatacg ttgccattaa cagacccagt aatccggtaa tgatcatcgc
     2701 ggaagtaatc attttgcggt gtgaaaattt atcggcaata acgccgctgg gggcataaag
     2761 aataatagcc gcaataccaa aggtactcat tattaaacct atctcggtat tgctgaatcc
     2821 cataaatttc g
//
LOCUS       DAZFRJ010000327         2775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000327 DAZFRJ010000000
VERSION     DAZFRJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.329"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            202..1368
                     /gene="nhaA"
                     /locus_tag="ACHKUN_004740"
     CDS             202..1368
                     /gene="nhaA"
                     /locus_tag="ACHKUN_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO6662841.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1434..2333
                     /gene="nhaR"
                     /locus_tag="ACHKUN_004741"
     CDS             1434..2333
                     /gene="nhaR"
                     /locus_tag="ACHKUN_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO6662842.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2372..>2775)
                     /locus_tag="ACHKUN_004742"
     CDS             complement(2372..>2775)
                     /locus_tag="ACHKUN_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO6662843.1"
                     /translation="SIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYY
                     FEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSN
                     QIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          650 a          614 c          705 g          806 t
ORIGIN      
        1 caccttaagc tatgattatc tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa
       61 actcatctca ccgctgatgg cgcaaattct tcaatggatc gtaaaaaacg agttattcct
      121 acactataat ctgattttaa cgatgatttg tgcggggtaa aatagtaaaa acgatctatt
      181 cacctgaaag agaaataaaa agtgaaacat ctgcatcgat tctttagcag tgatgcctcg
      241 ggaggcatta ttcttatcat tgccgctatc ctggcgatga ttatggccaa tagcggcgca
      301 accagtggat ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc
      361 gaaatcaaca aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg
      421 ttagtcggtc tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag
      481 gccgcatttc cagttatcgc cgctattggt gggatgattg ttccggcatt actctatctg
      541 gcctttaact atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac
      601 attgcctttg cacttggtgt gctggcgctg ttgggaagtc gtgttccgtt agcgctgaag
      661 atctttttga tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg
      721 ttctacacta atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta
      781 ctcgcggtat tgaatctgtg tggtgtacgc cgcacgggcg tctatattct ggttggcgta
      841 gtgttgtgga ctgcggtgtt gaaatcgggg gttcacgcaa ccctggcggg ggtaattgtc
      901 ggcttcttta ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat
      961 gtgttacacc cgtgggtggc gtatctgatt ttgccgctgt ttgcatttgc taatgctggc
     1021 gtttcactgc aaggtgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc
     1081 gctggcttgc tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt
     1141 ttgaaactgg cgcatctgcc tgagggaacg acttatcagc aaattatggc ggtggggatc
     1201 ctgtgcggta tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta
     1261 gatccagaac tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg
     1321 gtaattggat acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta
     1381 ccggggagcc ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc
     1441 atatcaatta caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg
     1501 gcgcagcgga ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg
     1561 aagagcgcct gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc
     1621 tgggagaact ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg
     1681 atattgtgaa ctatcgcaaa gaatccaatt tattgtttga cgttggtgtg gctgatgcac
     1741 tttccaaacg cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc
     1801 atcttcgctg cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac
     1861 tggatatgat catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg
     1921 tgagaattgg cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt
     1981 tcccggcttg tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc
     2041 gcaaattgct taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg
     2101 atgatgccgc gttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa
     2161 cgctttatgc atatgacttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga
     2221 atgtgatgga agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac
     2281 agcgaatctg caatacggat tattctgcgc tttttagtcc agcggcgcgt taatcggcac
     2341 ctccccccaa agttaaggtg ggggagatag attagttgta cataaccacg attttgactc
     2401 ggctcattat ttgcccgctt gagacattat ttccatatgt acgcgggcga ataaatagag
     2461 gaatctgatt acttccttca tggggatgct gaaaagagta gtaattgctt ttaccatact
     2521 ggatttcatt cgtaccatca ttggagagtt gaaagcctat ttgacccgca ccgtcctcac
     2581 tgttttctaa ctcatttttt aatatgctgg ttcctgactc ctgtatcgtc actggttcaa
     2641 agtaatattc aacatcactg ggttcaaaaa ctaattcacc ttgctcgccc tggatgcagt
     2701 caaatcttat atgatgaaac tgataatttt cattattgat acgagacagt acaaatcgtc
     2761 cagtgtcgat ggatt
//
LOCUS       DAZFRJ010000328         2772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000328 DAZFRJ010000000
VERSION     DAZFRJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.330"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..>2772
                     /gene="eae"
                     /locus_tag="ACHKUN_004743"
     CDS             2..>2772
                     /gene="eae"
                     /locus_tag="ACHKUN_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFO6662844.1"
                     /translation="MTHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENYF
                     KLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMMK
                     AGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDDK
                     ALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQS
                     GNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNVF
                     IDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRFN
                     GYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYRH
                     GTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKKQ
                     DILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQD
                     YQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTADK
                     TSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTLK
                     SATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTVR
                     VMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVGT
                     EVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSSN
                     TKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDAE
                     NKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSSTY
                     DLVTKNQLI"
BASE COUNT          807 a          542 c          659 g          764 t
ORIGIN      
        1 gattactcat ggtttttatg cccggacccg gcacaagcat aagctaaaaa aaacatttat
       61 tatgcttagt gctggtttag gattgttttt ttatgttaac cagaattcat ttgcaaatgg
      121 tgaaaattat tttaaattga gttcagattc aaaactgtta actcaaaatg ccgctcagga
      181 tcgccttttt tatacgttaa aaacaggtga aactgttgcc aatatttcta aatcacaggg
      241 tatcagttta tcggtaattt ggtcactgaa taaacattta tacagttccg aaagcgaaat
      301 gatgaaggct ggacctggtc agcagatcat tttgccactc aaaaaactgt ctgttgaata
      361 tagtgcctta cctgtcttag gttcggcacc tgttgttgct gcaggtggtg tcgctggtca
      421 tacgaataaa atgactaaaa tgtccccgga cgcgactaaa agcaacacga ccgatgacaa
      481 ggctctaaat tatgcggcac aacaggccgc gagccttggt agccagctcc agtcgcgctc
      541 actgaacggc gattacgcga aagataccgc tcttggtatg gccagcagcc aggcttcgtc
      601 acagttgcag gcctggttac aacattatgg aacggcagag gttaatctgc agagtggtaa
      661 taactttgac ggtagttcac tggacttctt attaccgttc tatgattccg aaaacatgct
      721 ggcatttggt caggtcggtg cgcgttacat tgactcccgc tttacggcaa atttaggtgc
      781 tggccagcgt tttttccttc ctgaaaatat gttgggctat aacgtcttca ttgatcagga
      841 tttttctggt gataataccc gtttaggtat tggtggcgaa tactggcgag actatttcaa
      901 aagtagcgtt aacggctatt tccgcatgag cggctggcat gagtcataca ataagaaaga
      961 ctatgatgag cgcccggcaa atggttttga tatccgcttt aatggctatt taccatcata
     1021 tccggcatta ggcgccaaac tgatgtacga acagtattat ggtgataatg ttgctttgtt
     1081 taattccgat aagttgcagt cgaatcctgg cgcggcgacc gttggtgtaa actacactcc
     1141 gattcctctg gtgacgatgg ggatcgatta ccgtcatggt acgggtaatg aaaatgatct
     1201 cctttactca atgcagttcc gttatcagtt tgataaaccg tggtctcagc aaatcgagcc
     1261 acagtatgtt aacgagttaa gaacattatc gggcagccgt tacgatctgg ttcagcgtaa
     1321 taacaatatt attctggagt acaaaaagca ggatattctt tctctgaata ttccgcatga
     1381 tattaatggt actgaacaca gtacgcagaa gattcaattg atcgttaaga gcaaatacgg
     1441 tctggatcgt atcgtctggg atgatagcgc attacgcagt cagggcggtc agattcagca
     1501 tggcggaagc caaagcgcac aagactacca ggctattttg cctgcttatg tgcaaggcgg
     1561 cagcaatatt tataaagtga ccgctcgcgc ctatgaccga aatggtaata gttctaataa
     1621 tgtacagctc actattaccg ttttaccgaa tgggcaggtt gtggaccagg ttggggtaac
     1681 ggactttacg gctgataaaa catcggctaa agcggatggc atagaagcta ttacctatac
     1741 cgcgacggtt aaaaagaatg gtgtagctca ggctaatgtc cctgtaacat ttagtattgt
     1801 atccgggact gcaactcttg gggcaaatag tgccagaacg gatggtaacg gtaaggcgac
     1861 cgtaacgctg aagtcggcta cgccaggaca ggtcgtcgtg tctgctaaaa ccgcggagat
     1921 gacttcgcca cttaatgcca gcgcggttat atttgttgat caaaccaagg ccagtattac
     1981 tgagattaag gctgataaaa caacagcgaa ggcagatggt tctgatgcga ttacctatac
     2041 tgtcagagtg atgaaggagg gggcacccgt agtagatcag aaagtgacct tttctaagga
     2101 ttttgggacc ctgaataaga ctgaagcaac aaccgatcag aatggttatg ctactgtaaa
     2161 attatcatcc aatactcctg gcaaggccat tgttagtgca aaagtgagtg gagtaggtac
     2221 agaagttaag gctactaccg ttgagttttt tgccccgttg agtattgatg gtgataaagt
     2281 gaccgtaatt ggtactggta tcacgggggc tctgccaaag aactggttac agtatggtca
     2341 ggttaagcta caggcaacag ggggcaatgg aaaatacaca tggaaatcca gtaatactaa
     2401 aattgcttct gttgataact cgggagtgat aaccttaaat gaaaaaggga gtgccacaat
     2461 tactgtagta tctggtgata atcagagtgc gacatacaca attaatgcac cgggtagtat
     2521 tgtaattgct gtggataaaa atactcgagt tacgtatttt gatgccgaaa acaaatgtaa
     2581 gacaaatagc gcaaatttag cacagtcaaa agaactattg gccaatatct attcaacatg
     2641 gggtgctgca aataaatatc cttactattc tggttctaaa tcattgactg cttggattaa
     2701 acaatcctct tctgaacagt catcaggtgt atcaagcaca tatgatttgg ttacgaagaa
     2761 ccagttgatc aa
//
LOCUS       DAZFRJ010000329         2766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000329 DAZFRJ010000000
VERSION     DAZFRJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.331"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..149
                     /locus_tag="ACHKUN_004744"
     CDS             <1..149
                     /locus_tag="ACHKUN_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO6662845.1"
                     /translation="FVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIF
                     LSRK"
     gene            424..1620
                     /gene="kbl"
                     /locus_tag="ACHKUN_004745"
     CDS             424..1620
                     /gene="kbl"
                     /locus_tag="ACHKUN_004745"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO6662846.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1630..2655
                     /gene="tdh"
                     /locus_tag="ACHKUN_004746"
     CDS             1630..2655
                     /gene="tdh"
                     /locus_tag="ACHKUN_004746"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO6662847.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          639 a          638 c          767 g          722 t
ORIGIN      
        1 tttttgtcat gtgctattac aaacgccccg atctcttcaa cctaaactat ctggggagag
       61 gaaaatggtt cgatttgttt cgctgcttca ggagtaacac tttaggggca aaaatgcagg
      121 cgctgagaat tttcctttca agaaaatagg tttccctcca ttagtgggta gcgttttgat
      181 accaactgag ataggaaaaa tgatgctttg gcatcatttt ttctttttat atcaatgcag
      241 taaaatcaat catcattatg cccagccaaa actgtacagt ttttctacta tctgtaggtg
      301 ttgagagaca tcaagttaca cgttatttat cctgaatttt gcagaagtgt taacgcgtca
      361 tctcgtcgcg acctataagt ttgggtaata tgtgctggaa tttgccctgt ctggagaatc
      421 gcaatgcgtg gagaatttta tcagcagtta accaacgatc tggaaaccgc acgggcggaa
      481 gggttgttta aagaagagcg cattattacg tctgcgcagc aagcagatat cactgtggct
      541 gatggaagcc acgtcattaa cttttgtgcc aacaactatc tcgggctggc gaatcatcct
      601 gatctgattg cggcggcaaa ggcgggaatg gattctcacg gtttcggcat ggcttcggtg
      661 cgttttattt gcggcactca ggacagccat aaagagcttg aacaaaaact ggcggccttc
      721 ctggggatgg aagatgcgat tctctactct tcctgctttg atgctaacgg tggcctgttt
      781 gaaacgctgc tgggcgcgga agacgccatt atctccgacg cactgaacca cgcgtctatt
      841 attgatggtg tgcgtctgtg taaagctaaa cgctatcgct atgccaacaa cgatatgcag
      901 gagcttgaag ctcgtctgaa agaagcgcgt gaagccggtg cgcgtcatgt gctgattgcc
      961 accgatggtg tattctcgat ggacggcgtg attgccaatc tgaagggcgt ttgcgatctg
     1021 gcagataaat acgatgccct ggtgatggta gacgactccc acgcggtcgg ttttgtcggt
     1081 gaaaatggtc gtggttccca tgaatactgc gatgtgatgg gccgggtcga tattatcacc
     1141 ggtacgcttg gtaaagcgct gggcggggct tctggtggtt ataccgcggc gcgcaaagag
     1201 gtggttgagt ggctgcgcca gcgttctcgt ccgtacctgt tctccaactc gctggcaccg
     1261 gccattgttg ccgcgtccat caaagtactg gagatggtcg aagcgggcag cgaactgcgt
     1321 gaccgtctgt gggcgaacgc gcgccagttc cgtgagcaaa tgtcggcggc gggctttacc
     1381 ctggcgggag ccgatcatgc cattattccg gtcatgcttg gcgatgcagt agtggcgcag
     1441 aaatttgccc gtgagctgca aaaagagggc atttacgtca ccggtttctt ctatccggtc
     1501 gttccgaaag gtcaggcgcg tattcgtacc cagatgtctg cggcgcatac ccctgagcaa
     1561 attacgcgtg cagtagaagc attcacgcgt attggtaaac aactgggcgt tatcgcctga
     1621 ggatgtgaga tgaaagcgtt atccaaactg aaagcggaag agggcatctg gatgaccgac
     1681 gttcctgtac cggaactcgg gcataacgat ctgctgatta aaatccgtaa aacagccatc
     1741 tgcgggactg acgttcacat ctataactgg gatgagtggt cgcaaaaaac catcccggtg
     1801 ccgatggtcg taggccatga atatgtcggt gaagtggtag gtattggtca ggaagtgaaa
     1861 ggcttcaaaa tcggcgatcg cgtttctggc gaaggccata tcacctgcgg tcactgtcgc
     1921 aactgtcgtg gcggtcgtac ccatctgtgc cgcaacacga tcggcgtcgg cgttaaccgt
     1981 ccgggctgct tcgccgaata tctggtgatc ccggcgttca acgccttcaa aatcccagac
     2041 aatatctccg acgacctggc ttccattttt gatcccttcg gtaacgccgt gcataccgcg
     2101 ctgtcgttcg atctggtggg cgaagatgtg ctggtttctg gtgcaggtcc gataggtatt
     2161 atggctgcgg cggtggcgaa acacgttggt gcacgcaatg tggtgatcac cgatgttaac
     2221 gaataccgcc ttgagctggc gcgcaaaatg ggtatcaccc gtgcggttaa cgtcgcgaaa
     2281 gaaaacctta atgatgtgat ggctgaactg ggcatgaccg aaggctttga tgtcggtctg
     2341 gaaatgtccg gcgcgccgcc agcgtttcgt accatgctcg ataccatgaa ccacggcggt
     2401 cgtattgcga tgctgggtat tccaccgtcc gatatgtcta tcgactggac caaagtaatc
     2461 tttaaaggct tgttcattaa aggtatttac ggtcgtgaga tgtttgaaac ctggtacaag
     2521 atggcggcgc tgattcagtc aggtctggat ctctcgccga tcattaccca tcgtttctct
     2581 atcgatgatt tccagaaggg ctttgacgct atgcgttcgg gccagtccgg gaaagtaatt
     2641 ctgagttggg attaacacga acaagggctg gtattccagc ccttttgcct gaggataatc
     2701 cgttaaatat gtaaaatcct gtcagtgtaa taaagatatc gtaattgcgc tgatctctta
     2761 tatagc
//
LOCUS       DAZFRJ010000330         2760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000330 DAZFRJ010000000
VERSION     DAZFRJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.332"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..344
                     /locus_tag="ACHKUN_004747"
     CDS             99..344
                     /locus_tag="ACHKUN_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662848.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            475..669
                     /locus_tag="ACHKUN_004748"
     CDS             475..669
                     /locus_tag="ACHKUN_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662849.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            673..834
                     /locus_tag="ACHKUN_004749"
     CDS             673..834
                     /locus_tag="ACHKUN_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO6662850.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            962..1450
                     /locus_tag="ACHKUN_004750"
     CDS             962..1450
                     /locus_tag="ACHKUN_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6662851.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            1462..1623
                     /locus_tag="ACHKUN_004751"
     CDS             1462..1623
                     /locus_tag="ACHKUN_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662852.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            1613..2536
                     /locus_tag="ACHKUN_004752"
     CDS             1613..2536
                     /locus_tag="ACHKUN_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6662853.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
BASE COUNT          906 a          595 c          671 g          588 t
ORIGIN      
        1 catattgaaa aacataacgt tagaatcaca aaaaaaagag gtggggagac ggtgggtaat
       61 ttcaaaaagt ggggagcaaa agtggggagt agtggggaat gaacagaaaa accagagata
      121 agacagcgcc aaaatataaa gcgttagaca tgacagagct ttccttaaag gtggcaatca
      181 gaacgataga ccgccacgcg ggggaaggat acgcgaagga gcatcccgaa ctgataagcg
      241 cattcatgac cacagcggcg gcaaactttg ccacgctgac agaacgggag attgccgaag
      301 cggaacaggt aacaaccatc aacgttaaaa gcgctggaga ataaccgaag aagtgagcac
      361 atagccggag caatccggct ttttttcgat ctcatatatc ttcaaagatg gttaattaat
      421 gataatctat cgaaaatact ggttatgcaa tgataagtat caggagaaaa caaagtggaa
      481 gagataaagc aaagcgctac cgtgatgatt gatcatgaaa tccttaacga gcttaaaaag
      541 ttgcagttcg atattgtgcg ccggatgaat gaatcaggta tctacggcag ggcggcaaca
      601 ccaacaatag ggcatcttgc gcgcgtactg ttacgcgaac gcctgggcat tgcaacaagt
      661 gaggcgtaaa caatggctat cacagaacgc gaggcaagca tcattaaagc cattggcgag
      721 gaaacaagag acaccatagc gccattgatg gaaaagatcg cagagcttga gacacgaata
      781 aacaagctga gtgatgaagt tgaaggcctg atctcgttac tcggtgagca atgacagggg
      841 gggagtgttc cggccggaaa gtcgatcgcc ctggacaccg cccccagtct cattcataga
      901 aaattttgct tttcaaaaaa gttacacgaa aagttacacc ccctcaaaac gaggtttttg
      961 catggcatta acgaccaaaa aaaagcggtt tgctgatgcg ttatcaaagg gcacaaatcc
     1021 aacggaagcc gcgatacatg cgggatacag cgaaaagact gcaagggtta aaggctggca
     1081 gctatccaat gatccggagg tgaaaaagta tcttaaggct gtaacttctg atgtaacttt
     1141 tcaggttaca aaaccgaaaa aagttacaaa gaaagttacc gaaaaagtta caccggaacg
     1201 ggcatcaatt aacaccattg agatgatgga taacggccta cccgacccca tcaaagcaat
     1261 gggcaggata ctgattgaca acatggacac agatccaaaa ctggctttag atgccgcgtt
     1321 taagctggcc cagttcaccc accgcaagat cggcagcatt gggaaaaaag aggttaaaaa
     1381 actcaatgcc gctaatgtct cctcaaatcg gttccccatc cctaagccac caactggctt
     1441 aaaacactga ggtagcgaac tatgagcgaa tacatcacag accctattga tgtactcacg
     1501 gaaatcatga acgacaagca gcagccaacc gaaatacggt tacaggctgc cgctgttctg
     1561 atgccatatt ttcacgaaac cctaacccca tgttaccagg aggaagaaga taatgatttc
     1621 taaaaacatg aaaggcgtgt cggcagttcg cgccgccagc gtgcttacat cgtgtaaatt
     1681 cagccatgca gacgcaattg aaatgacgaa aaaaaatgat ccggcagtat cgaaatttct
     1741 tgaaaaatcc attgttgccg gaatgactac ggataatatg ccgtcgctgg tggcagagcc
     1801 gatagtaaga gagcttggcg agtatatttt ttcgcagtct gtaccaggca agctaatcag
     1861 cagcgcgctg aatcttccgt ttggcgcaaa attgcagtca atatcaggca agggtagtaa
     1921 atggcttaaa gaagccgaag aaatccctgt caaagaggga gaagtaaacg aaagtgcgct
     1981 aaaactgtgc aaacttggcg ctattgtgat gctgaataac gagctaatca aatttagcac
     2041 ccctggtacc aattcagcga tcagagactt gatcacatcg gaatcggtga aagaaataga
     2101 taaaaaattt ctttccagtg accaggaggt ggcgggcgta tcccctgagg gcgtgcttaa
     2161 tggtgctgat gaggccgaaa acttcataga gcttttcaaa aagcacatag cgaacggtaa
     2221 cacgcttgcc acgtcgtcgc taatcttgcc cgtagaaaac gccttacagc ttactgatgc
     2281 tgagttctgg cagatagagc ttttgagaat accggtaatt gtgagcgaac aggcggatcg
     2341 catgatgctg atagacgctc aaaagctgat tattaacgtt gaagccacgg ttataacccc
     2401 aactgatgag gcggtaataa aaaccgaatc agggacggtt agccttttcc agaatgacgc
     2461 tacagcgttc agggcaatca cctattgcgg atggcaaaaa ctcgataagg ccgtcaccgt
     2521 tctggcgaaa agctgatttt tccggcatca atcccgatat ggtgatcccc atatcgggat
     2581 aattacgaaa ttttcgcagt ttaattaatg gtcagattaa tcgctaaccc gctgatattt
     2641 tcggaaacct caatttgagg aagtcggaag cgtcaccctt caaatcctga atggtcataa
     2701 tgaccacgca gaccatcaag gcaaccataa cgaacatcgt tacggttgga gcttttacca
//
LOCUS       DAZFRJ010000331         2725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000331 DAZFRJ010000000
VERSION     DAZFRJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.333"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>2725)
                     /locus_tag="ACHKUN_004753"
     CDS             complement(<1..>2725)
                     /locus_tag="ACHKUN_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6662854.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFR
                     QLALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYICSVNTVGK
                     SAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVS
                     NEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIE
                     NTSDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTV
                     GKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          536 a          853 c          706 g          630 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggcttaat attgatactg gcagctatcc agtacagcgc cgtgccaaga tagcgggctg
      961 tggtttcaac ctgcctgata tccgcaatcc gcttttccga gaaccagaat tcaaactgta
     1021 ccgtcggatc ataaaccgca agatgcggcg tggcggttat ctgaaaatag cccggcgtca
     1081 gctcaatccg cgacggcgct gccggtgcgg caatccggaa cgataccgac gccggatcgc
     1141 cctgctgtcc ccacgcattt accgcccgga ctgtcagcgt gtaacgcccc agcgccagtt
     1201 gcctgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     1261 catccgctgc cacggtcagg cgaagaagga agctaacgcc cttcaccacc ttcggcgtgt
     1321 cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggca gtcaggtgct
     1381 gtaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca aagtgcgccc
     1441 cgttatccac gatggcttct ttttccggta catgctgcac ggcagtgatg gcatacgtgc
     1501 cgtcatcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc gtcggcaact
     1561 tcagccccca tacgctgtat tcagcaacgc cgtcaggaac acggctcact ttcaccttca
     1621 cgccgtcggt gacggactga acctccacgc tgaccggatt gccacttccg tcaaccaggc
     1681 ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg
     1741 tctggctgtt caccgccagc acgcgcccgc cggtgctgat accggcatag tcatcatcgc
     1801 agatttcaat gacatcgccc ggcacatggc gaagcccttc tgcgcccacg ctgaagtcca
     1861 cggtctgcgt ttccagcagt tctgttttaa tcagccacag cccggcgcgg tgtgcctgcc
     1921 cccggctggt acagccaaaa gcatccatct tcgtgacgtt acgaccgtaa cgggcaatgg
     1981 cctgcgtatc ctccacaagc tctgtcgccg tctcccagcc gttgttcggg tcaatccagt
     2041 tcacctcaac ggcattatgg cggtccttca gggcgctgaa gctgtagcgg aacggcgcgc
     2101 catcatccgg catcaccaca ttactgcggt tataggtcca caccttatcc gacggtcggt
     2161 cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc gccgagcaga
     2221 aatcactgag cacatcccac gccttgcgct gtgtggtcag gtacgcatta caggtgatgc
     2281 gcagctccgt gccgccaaag ccgtccggca ctgactggtc gcagtactgg ccgatgacat
     2341 acagcgccca tttgtccaca tccgccgcac caagacgttt ccccatgccg tagcgcgggt
     2401 gggtcagcat atcccacaga caccaggcca tgttgttgct gtatgctggt tttaacgttc
     2461 cgtcccagat accgctgtat tgtcgcgtct gcgggttata gttcgacggc acctgcagaa
     2521 tgcgcccgcg aagatgataa ttacgactca cctgctggct gccgaactgc tccgagtcca
     2581 cctgtacgcc gaccagtgcc gtgttcgggt agcactgttt cacatcgatg atttcggtgt
     2641 atgacgacca gagcgttttg ttctgcagct ggtctgtggt gctgtccggc gtcatcctgc
     2701 gcatccggat attaaacggg cgcgg
//
LOCUS       DAZFRJ010000332         2717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000332 DAZFRJ010000000
VERSION     DAZFRJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.334"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..248
                     /locus_tag="ACHKUN_004754"
     CDS             <1..248
                     /locus_tag="ACHKUN_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO6662855.1"
                     /translation="MLQDKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIE
                     AAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            276..1234
                     /locus_tag="ACHKUN_004755"
                     /pseudo
     CDS             276..1234
                     /locus_tag="ACHKUN_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1246..1707
                     /gene="yibG"
                     /locus_tag="ACHKUN_004756"
     CDS             1246..1707
                     /gene="yibG"
                     /locus_tag="ACHKUN_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO6662856.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSA
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2342..>2717
                     /locus_tag="ACHKUN_004757"
     CDS             2342..>2717
                     /locus_tag="ACHKUN_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO6662857.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVN"
BASE COUNT          863 a          489 c          616 g          749 t
ORIGIN      
        1 agatgcttca ggataaaaat gaagaagtcc gtattgaagc aataatcgga ctttcctaca
       61 gaaaagataa aagggtttta tctgttttat gcgatgagtt aaaaaaaaat actgtttatg
      121 atgatatcat tgaagctgcg ggtgaattag gtgataaaac gctacttcct gttttagata
      181 ctatgttgta caagtttgat gacaatgaaa ttataacttc cgctattgat aagctgaagc
      241 gttcatagcg tgagttgcct atgcacagtg ggggattccc gccggcacgg tgcagatgct
      301 cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt gaggaaagcc gccgctggct
      361 ggcatcgtgc ggcctgaccg tggagcagat gaaaaaccag atggacccgg tgtacacgcc
      421 ggcgcgaaaa atccacctgt accactgcga ccatcgcggc ctgccgctgg cgctcatcag
      481 cacggaaggg gcaacagcgt ggtgcgcaga atatgatgaa tggggcaacc tgctgagtga
      541 tgagaacccg catcatctgc agcagctcat tcgcctgccg ggtcagcagt atgatgagga
      601 gtccggcctg tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac
      661 tcaggatccg attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt
      721 cataaatgta gatccgcaag gtttggttga tataaattta taccccgaaa gtgatcttat
      781 ccattctgta gctgatgaga ttaatatccc aggcgttttc acaatcgggg ggcatggtac
      841 ccccacatct attgaatccg caacgcgcag tatcatgaca gctaaagatc tagcatatct
      901 aattaaattt gatgggaatt ataaagatgg gatgacagtt tggttatttt cttgtaatac
      961 aggtaaagga caaaattcat ttgctagcca attagctaaa gagttacata caaatgtaat
     1021 aggacctgac acgctatgga cgtggtgggg gcgaggaact aatggtaagt taaaaatgga
     1081 tacagtgcta acagcaccaa cgaaccttaa ttcaaataag gatctaatgg ctataacaac
     1141 aaaagacctt ggtaattgga taacatatgg gccatctggg caccccattt ctaatatgca
     1201 aggtacgcca gaaaaaccca gtgatataag ataggttgta gatgtatgaa agcatgcttg
     1261 ttactatttt tttatttttc tcttattggt caattgcatg gtgctgatgt gaaaataaaa
     1321 gaaaacgaaa gtgtgatggg atctacagca atgacctatg atttaagtga agaaaagctg
     1381 atgaaactga agtataaatc acaacatggt gattcagagg catcatttcg actatatcaa
     1441 tattactgct ttactaaaaa taatattgat aaacaattgc gattcttgga aagatcagca
     1501 tctcagggga atgttacagc gcagtttaat tatggggtct ttttatcagc tacaaatcca
     1561 acattatcag aatattataa tttgaataga gctatttatt ggatggaatt cgctgtaaat
     1621 aacggtaata ttgatgcaaa gagcaaactt caagagctca aaaagctaaa gcgaatggat
     1681 agaaggaaga ataaggagaa tccgtaaaag caatagttca tcagactgcc gcagcagtag
     1741 tatgatgatg aatcagggct gtactataac tattatcggt aacattaacc gcagcagggg
     1801 cggtataaca ctcaggctcc gattgggctg gaaggtaggt ggaattttta tcagtatccg
     1861 ttgaacccga tttctggaat cgatcccctt gggattagcc acctgtttat acagtataac
     1921 tttaggtatg ctttcgtgtg tttcagatat accatgtgat gataattttt atgatgtgct
     1981 tagtattcca gtcgcttctg gtaataatat gcaatgtaaa aataatcccc gttgtacaca
     2041 tttataaata gatggccaat acctcaaggc ctatggagtt agaatgttaa tgacccggga
     2101 gcaagcaata gaaaacctga cggcatacga ttagtaccgt cagttaatac aggaacttat
     2161 aatcgcaatg gattttcaat acatagttgt ttaaacgctt ttggtccgtc tttaggacct
     2221 cgattctgtt ccgagggttg tataactggt ttatctaatg acatgcaaaa attaaatgag
     2281 ttaatatttt cagagccaga tagcacatta acagttacgg actaaaaaat atggcaaggt
     2341 tattaacaat tgtatttttt tctctatttc tgcgagtgca gttactgcaa gacaatttca
     2401 gagagagtta gatttggcag acaaagcaat attatgggca ctaatatcag ccagtactaa
     2461 agagggacga aaagcgtgtt cactaagcta ttttgcctgt aaggccgcag aggctgaact
     2521 cggactggca tatatggctg cgaatgataa caaggaattt ctcacatcct tatctaatat
     2581 aatgaggtac aaaatagatg ctggactttc cgaatcctat acatgctatt tattaagtaa
     2641 gggaaagatc ataagaccat atctgaaaaa tctaaatcca ctccaacttg ccgctgattg
     2701 cattgaaaca gtaaata
//
LOCUS       DAZFRJ010000333         2713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000333 DAZFRJ010000000
VERSION     DAZFRJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.335"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(253..1107)
                     /gene="rclR"
                     /locus_tag="ACHKUN_004758"
     CDS             complement(253..1107)
                     /gene="rclR"
                     /locus_tag="ACHKUN_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO6662858.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1333..2658
                     /gene="rclA"
                     /locus_tag="ACHKUN_004759"
     CDS             1333..2658
                     /gene="rclA"
                     /locus_tag="ACHKUN_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO6662859.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          784 a          594 c          629 g          706 t
ORIGIN      
        1 acttattcag gggttattga gatatacact gatggtgtac aaaaaaatca ccgacaataa
       61 tcacgtatca agtaatagtc ggggttttat ttaattgaag caatttagtt tgcattttta
      121 tttaaaaact gagctagagt ccatcagaaa aaaatatata aagcccaaac caccgttgga
      181 aatagaaata gttgaaaggc tcacaactcc cggaatgcgg attatctttg ccctgatttc
      241 tggcggtttt attcagggtg caagctgtct gaccctttcc cgatattctc ccggagtaca
      301 accaaactcg cggacaaacg ccttgtgaaa agatgattca ctggcatagc ctactgactc
      361 agcgatcacc acaacaggga gcatttcccg ggaaaacatc tgggccgcta tttgtagacg
      421 caactttgtt aatacagcca gcggcgtggt tccggaaaca tcacggaaaa gctgggcaaa
      481 acttgcccgg gacatgtgag cgatgctggc cagcgattcg acggtccagg cgtgtcctgg
      541 catttccagc atttgctgta ttaccgcacc aagacgtgga tgcagaagca aactgagaat
      601 gtttttctca gtattaacct gtgcaatcca ctcacgcacc gcgagggtaa agaatgtagc
      661 gcagatctgg ctacacagtg catccactcc cggcatcgcc gatctggatt cctgttgtaa
      721 aaacgggatc gcctccctca gccagttata ttccacgctg tggttaaccg gtgctaaaaa
      781 cagcgtttcc ggcaaagacg ttaaaaaata acgcgccgaa tgttgcaacc gaagagtgcc
      841 gcagacaata caggtcgatt cattatcgac atgactcagg cgatgagctg aattttgtgg
      901 tagcaggacc acatttcccg ggcgtaatgt aaaaatctct cccgtcggca tttccagctt
      961 cgccgctcct tgcgttaacg catgccaacg aataaccgat aattccccgg caccatgtgg
     1021 aagctgccag tcacttccta acacgcaatt cttatcgatc gttccttgtg gagcgttaag
     1081 catcaacaaa cggctaaggg catccatatc agactcctga gtggtttttg attacaaaca
     1141 ccaacaatct tagtaaataa ctaatctaat accatcaaat ggttaatcat gatttttgtt
     1201 ttgtgattct gattaagaaa aagaaaatac cagacgataa gacaaaatat caagcgttgt
     1261 gaagaaatgg tatttgctct tttgcgtcta tagtcatgat gtcaaatgaa cgcgtttcga
     1321 caggaaatca tcatgaataa atatcaggca gtgattattg gttttggcaa ggctggaaaa
     1381 acattagccg tcacgctggc aaaagcaggt tggcgagtgg ctctcatcga acaatcaaat
     1441 gcaatgtatg gcgggacctg tattaatatc ggctgcatcc caaccaaaac attggttcat
     1501 gacgcacagc agcacacaga ttttgtccgt gccatacagc gtaaaaatga agtggttaat
     1561 tttttacgta ataagaattt tcataatctt gcggatatgc ccaatatcga cgtgatcgac
     1621 ggccaggcgg agtttatcaa taatcatagc ctgcgtgttc atcggcctga ggcaaatctg
     1681 gaaattcatg gcgagaaaat ttttattaat accggtgcac aaaccgtggt tccgccaatt
     1741 cctggaatta ccaccacgcc aggagtatat gacagcaccg gattacttaa tctaaaagaa
     1801 ttgcctgggc atttaggtat tttgggcggc ggatatattg gcgttgagtt cgcctctatg
     1861 ttcgctaatt ttggcagcaa ggtaaccatt ttagaagcag cttcgctgtt tttgcctcgg
     1921 gaagatcggg atattgctga taatatcgcg acgattttac gcgatcaggg cgtcgatatt
     1981 atcctcaatg cccatgtgga gcgaatcagt caccatgaaa atcaagtgca agtgcatagc
     2041 gagcacgccc aactggcggt ggatgcactg ttaatagctt ccggtcgtca accggctacc
     2101 gcttcgttac atccagaaaa tgccggtatc gcagtaaacg agcgcggggc aattgtcgtt
     2161 gacaagcaat tacataccac cgcagacaat atttgggcga tgggagatgt taccggcggg
     2221 ctgcaattta cttatatttc actggatgat taccgcattg tgcgtgatga gttgctggga
     2281 gaaggcagac gtagtactga tgatcggaaa aatgtgcctt attccgtatt catgacaccg
     2341 ccactgtcca gggttggtat gacagaagaa caggccagag agagtggtgc tgatattcag
     2401 gtggtgacat tgcctgtagc tgcaattccg cgtgccagag tgatgaatga tactcgtggg
     2461 gtattaaaag cgattgttga taataaaacc caacgtatat taggggcatc actgctgtgt
     2521 gttgactccc acgagatgat caatatagtg aaaatggtga tggatgccgg gctgccttat
     2581 agcatattac gcgatcagat atttactcat ccgtcgatga gcgaatcact caatgatcta
     2641 ttttcattag tcaaataaac tcaaaatcag acgccagaac aaatattctg gcgtctcaga
     2701 gaaaagaatc tta
//
LOCUS       DAZFRJ010000334         2703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000334 DAZFRJ010000000
VERSION     DAZFRJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.336"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..569
                     /locus_tag="ACHKUN_004760"
     CDS             <1..569
                     /locus_tag="ACHKUN_004760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6662860.1"
                     /translation="YFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYL
                     KLQSLFARVAEEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALG
                     RRATGVELETERFEQTVREVQDLASQNG"
     gene            610..685
                     /locus_tag="ACHKUN_004761"
     tRNA            610..685
                     /locus_tag="ACHKUN_004761"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:643..645,aa:Ile,seq:cat)
     gene            695..771
                     /locus_tag="ACHKUN_004762"
     tRNA            695..771
                     /locus_tag="ACHKUN_004762"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:729..731,aa:Arg,seq:tcg)
     gene            785..861
                     /locus_tag="ACHKUN_004763"
     tRNA            785..861
                     /locus_tag="ACHKUN_004763"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:819..821,aa:Arg,seq:tct)
     gene            952..1911
                     /locus_tag="ACHKUN_004764"
     CDS             952..1911
                     /locus_tag="ACHKUN_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO6662861.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1923..2192
                     /locus_tag="ACHKUN_004765"
     CDS             1923..2192
                     /locus_tag="ACHKUN_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO6662862.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(2490..>2703)
                     /locus_tag="ACHKUN_004766"
     CDS             complement(2490..>2703)
                     /locus_tag="ACHKUN_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871291.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6662863.1"
                     /translation="PPGCIFTPTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQEC
                     PANTGNITDKCFNHRCILEICPYGRK"
BASE COUNT          690 a          579 c          718 g          716 t
ORIGIN      
        1 cttactttcg tgatgcgcgt gctgccctgg ggataacggc aaaacagata gtggatgcca
       61 caggaaagaa aaacatggtg tcgcactggt tcagtgccag tcagtggcag ctaccgaacg
      121 aaagcgatta tctgaaatta cagtcgctgt ttgcccgggt ggcagaagag aagcatcagc
      181 gcggtgaact ggaaaagccc caccaccagc tgctggagac gtatacttca ctgaaccggc
      241 agtatgcgga actgcagagt gaatataagc atctgcggcg gtattttggc gtgacggcgc
      301 aggtgccgta cacggatgtg tggacgcata aaccggtgca gtactatccc gggaaacatc
      361 cgtgcgaaaa accggcagaa atgctgcagc agataatcag cgcgagcagt cgtccgggtg
      421 acctggttgc agattttttc atggggtcgg gttcgacagt caaagctgca ctggcgctgg
      481 ggcgtcgtgc aactggcgtt gagctggaga ctgaacgttt tgagcagacg gtcagggaag
      541 ttcaggattt agccagtcag aacggatgat attgcaggat tagttacgta ccgttattat
      601 cctgcgcccg gccctttagc tcagtggtga gagcgagcga ctcataatcg ccaggtcgct
      661 ggttcaaatc cagcaagggc caccatatca cataccgcca ttagctcatc gggacagagc
      721 gccagccttc gaagctggct gcgcggggtt cgagtcctcg atggcggtcc attatcggta
      781 ttcagcgttg ttagctcagc cggacagagc aattgccttc taagcaatcg gtcactggtt
      841 cgaatccagt acaacgcgcc atatttattt accaggctcg cttttgcggg ccttttttat
      901 atctgcgccg ggtctggtgc tgattacttc agccaaaagg aacacctgta tatgaagtgt
      961 atattattta aatgggtact gtgcctgtta ctgggttttt cttcggtatc ctattcccgg
     1021 gaatttacga tagacttttc gactcaacaa agttatgtct cttcgttaaa tagtatacgg
     1081 acagagatat cgacccctct tgaacatata tctcagggga ccacatcggt gtctgttatt
     1141 aaccacaccc cgccgggcag ttattttgct gtggatatac gagggcttga tgtctatcag
     1201 gcgcgttttg accatcttcg tctgattatt gagcaaaata atttatatgt ggccgggttc
     1261 gttaatacgg caacaaatac tttctaccgt ttttcagatt ttacacatat atcagtgccc
     1321 gatgtgacaa cggtttccat gacaacggac agcagttata ccactctgca acgtgtcgca
     1381 gcgctggaac gttccggaat gcaaatcagt cgtcactcac tggtttcatc atatctggcg
     1441 ttaatggagt tcagtggtaa tacaatgacc agagatgcat ccagagcagt tctgcgtttt
     1501 gtcactgtca cggcagaagc cttacgcttc aggcagatac agagagaatt tcgtcaggca
     1561 ctgtctgaaa ctgctcctgt gtatacgatg acgccgggag acgtggacct cactctgaac
     1621 tgggggcgaa tcagcaatgt gcttccggag tatcggggag aggatggtgt cagagtgggg
     1681 agaatatcct ttaataatat atcagcgata ctgggtactg tggccgttat actgaattgc
     1741 catcatcagg gggcgcgttc tgttcgcgcc gtgaatgaag agagtcaacc agaatgtcag
     1801 ataactggcg acaggcccgt tataaaaata aacaatacat tatgggaaag taatacagct
     1861 gcagcgtttc tgaacagaaa gtcacagttt ttatatacaa cgggtaaata aaggagttaa
     1921 gtatgaagaa gatgtttatg gcggttttat ttgcattagt ttctgttaat gcaatggcgg
     1981 cggattgtgc taaaggtaaa attgagtttt ccaagtataa tgaggatgac acatttacag
     2041 tgaaggttga cgggaaagaa tactggacca gtcgctggaa tctgcaaccg ttactgcaaa
     2101 gtgctcagtt gacaggaatg actgtcacaa tcaaatccag tacctgtgaa tcaggctccg
     2161 gatttgctga agtgcagttt aataatgact gaggcataac ctgattcgtg gtatgtgggt
     2221 aacaagtgta atctgtgtca caattcagtc agttgacagt tgcctgtcag actgagcatt
     2281 tgttaaaaaa atttcgcatg gtgaatcccc ctgtgcggag gggcgactgg tgtataaggt
     2341 gtcatctctg acgacaacct ggatcgagat acgcgggttc agggacaccg gactgaactc
     2401 accgggaggc acccggcacc atgttcatgg tgatacagaa atgcggcttc agcccctctc
     2461 cggaggggct ttttatctga atgattctgt tattttcgcc cgtatgggca tatttccaga
     2521 atgcagcgat gattaaaaca cttatctgtg atatttcctg tgtttgcagg gcattcctgg
     2581 ctgcttttaa ttaaattcca gacgttttta ttaaatgggg tcacgttgta aatggtgacg
     2641 gggagctctt tattattgag tattacgcct gtccgggttg gtgtaaaaat acagcccgga
     2701 ggg
//
LOCUS       DAZFRJ010000335         2701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000335 DAZFRJ010000000
VERSION     DAZFRJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.337"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..101)
                     /locus_tag="ACHKUN_004767"
     CDS             complement(<1..101)
                     /locus_tag="ACHKUN_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFO6662864.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALDLKGS"
     gene            536..1042
                     /locus_tag="ACHKUN_004768"
     CDS             536..1042
                     /locus_tag="ACHKUN_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6662865.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTGFCIF
                     ALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLD
                     EYIRATTE"
     gene            1014..>2701
                     /locus_tag="ACHKUN_004769"
     CDS             1014..>2701
                     /locus_tag="ACHKUN_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6662866.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPSPADEATSYAIRFPDDPEIFSQTEAQQLVA"
BASE COUNT          672 a          580 c          736 g          713 t
ORIGIN      
        1 gaacctttca ggtcaagagc gatgttaatt tgttcaatta tctggtttgg aaatcggatg
       61 ttgcgggttg ttgttctgcg ggttctgttc tttgatgaca taatgttgcc ccgtattcag
      121 tgttgctgat ttgtattatc tgaagttgct tttacgctaa tttgatgcag atcaattaat
      181 acgatacctg cgtaataatt gattatttct cgtggtttga tggcgtacac acatgtcgtg
      241 ataaacctca tgtagatgat aattattatc attttcgtgg gtcctttccg gcgatccgac
      301 aggttacggg gcggcgacct cgcgggtttt cgctatttat gagatttttt gagggggagt
      361 tgttgtttaa ttgtttggta tatctaattg ataagtaagg tgaaaataaa ataaatacaa
      421 caaccttacg atgtgttttg atgtcgtcaa tgcgaaaaat gtcaatgata tcaaatggtt
      481 ttgtaaaaac acatggttgt tgtatcgctt tttatcgatg gcttatggag aggagatggc
      541 ctttttattg aataaaagtg atatggcctc ctccatcggt atatctgttc aggcatttga
      601 taaatggggc gttcctcctg ttgagcgtcg ggggagggaa gttttttatg acgttaaaac
      661 tgtactggag atagatcgcg agcggcgaca acacaatcag agaatacctg atgacgaggg
      721 cgatctggag gaaaggctgc ttcgggccag agctgaactg acagaagaac aggccgtagc
      781 tcaaaaactt aaaaatcagg taaccgaagg taagcttatt gacaccggat tctgtatttt
      841 tgccctcagt aagctggcaa tggcgttatc cagtacgctt gattccatcc ctttatccat
      901 gcagcgacag tttcctgatt taacaccgcg ccatcttgac catctgaaaa cccttattgc
      961 taagggggca aatcagtgtg cgcgggcagg ggataaatta ccggatttac ttgatgaata
     1021 tatcagagca acaactgaat aatatgatga gcgctgtcac aactgcatta cagcccctga
     1081 taagggcatt gccggtgacg ccagttgaat gggctgatca aaattattat ctgcctaaag
     1141 aatcttcata tggtgagggc gaatggaaaa cgctgccatt ccagatcgcc atcatgaact
     1201 gcatggggaa tgatcagatc cgcactgtta atctgattaa atctgcccgt gttggctata
     1261 caaagatgtt gctgggggtg gtcgggtatt ttattgagca taaatcccga aacagtctgc
     1321 tttttcagcc cacggattct gccgctgaag attttatgaa gtctcacgtg gaggcgacga
     1381 ttcggaacgt gccatgcctg aaagaccttt ccccatggct gggtcgtaaa catcgtgaca
     1441 atactctcac gctgaaacgc ttttcatcgg gcgtcggttt ctggtgcctg ggcggcgctg
     1501 ccgccaaaaa ctaccgtgaa aaatccgtgg acgtggtctg ctatgacgaa ctttcctcgt
     1561 tcgagccgga tgtcgaaaaa gagggctcgc caaccctgct gggggataag cgtattgagg
     1621 ggtcggtgtg gccaaaatcc attcgcggct cgacgcctaa aatcaaaggc acctgccaga
     1681 tcgaaaaagc cgctaacgag tcggcgcatt ttatgcgttt ttatgtgccc tgcccgcact
     1741 gtggggagga gcagtatctg aaatttggcg atgagtccac gccttttggg cttaaatggg
     1801 agaaggacag ccctgaaagt gttttctacc tctgtgaaca tcatggctgc gtgatccatc
     1861 agtctgaact ggaccagagc aacgggcggt ggatctgtga aaacacgggc atgtggaccc
     1921 gtgacggtct gacgtttttc agcgcccggg gtgatgaaat tccgccgccg cgctccatca
     1981 cgttccatat ctggacggcg tacagtccgt tcaccacctg ggtacagatt gtctatgact
     2041 ggctggatgc actgaaagat cccaacggcc tgaaaacctt tgtgaacacc acgctgggcg
     2101 agacctggga agaggccgtg ggcgaaaaac tcgatcacca ggtactgatg gataaggtgg
     2161 tgcgttacac ggcggcggtg cctgcccggg tggtttatct gacggcgggc attgactcgc
     2221 agcgaaaccg ttttgagatg tatgtctggg gatgggctcc gggagaggaa gcctttctgg
     2281 tggataaaat catcattatg gggcgtcctg atgaggaaga gacgctgtta cgtgtggatg
     2341 cggcgatcaa caaaaaatac cgccatgcgg atggcaccga aatgactatt tcccgtgtct
     2401 gctgggacac cggggggatc gatggtgaaa ttgtttatca gagatcaaaa aaacacggtg
     2461 ttttccgggt gctgccggta aaaggcgcat ctgtctatgg caagccggtg atcaccatgc
     2521 caaaaacccg caatcagcgg ggcgtgtatc tgtgtgaagt gggaacggac accgcaaaag
     2581 aaattctcta tgcccgtatg aaagccgatc cctcgcctgc ggatgaagcc acgtcgtatg
     2641 ccatccgttt tcctgatgat ccggagattt tttcgcagac agaggcgcag caactggtgg
     2701 c
//
LOCUS       DAZFRJ010000336         2697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000336 DAZFRJ010000000
VERSION     DAZFRJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.338"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            446..661
                     /locus_tag="ACHKUN_004770"
     CDS             446..661
                     /locus_tag="ACHKUN_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6662867.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            661..1158
                     /gene="rrrD"
                     /locus_tag="ACHKUN_004771"
     CDS             661..1158
                     /gene="rrrD"
                     /locus_tag="ACHKUN_004771"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO6662868.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            1155..1592
                     /locus_tag="ACHKUN_004772"
     CDS             1155..1592
                     /locus_tag="ACHKUN_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6662869.1"
                     /translation="MSRVTVIISALVICIIACQSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTSDAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            1742..2359
                     /locus_tag="ACHKUN_004773"
     CDS             1742..2359
                     /locus_tag="ACHKUN_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6662870.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(2547..>2697)
                     /locus_tag="ACHKUN_004774"
     CDS             complement(2547..>2697)
                     /locus_tag="ACHKUN_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6662871.1"
                     /translation="RFPNQMIEQINIALEQKGSGNFSAWVIEACRRRLTSEKRAYTSI
                     QSDDG"
BASE COUNT          730 a          595 c          693 g          679 t
ORIGIN      
        1 cataataaaa aaagagccag cgactgacct gaaaaagaag acgctggcta aaaggcctta
       61 tatgtttgta gagacttatt tttcacagac agcaatgatg cctgtcaata tattatcaat
      121 atgcggattg tttcagttac agatgcctta ttaaggaaaa aaacagccag cactgacttt
      181 cggtggagag gtgctggctc aaaaggatag atgtacttca catgttgctt ctatatggca
      241 gtacattttc tgacagacag tgacggatgt tgtcaagata ttgtgtcatt tataacctga
      301 atcaggggag gccggaatgt tatctggcat ttttagcaga gcctgaatgc cataatcacg
      361 gctcccggcg ttggccgtca gtgggtgaca ctggcggctt ttttgttttt ctttactttc
      421 attttctgtc ggcggtgacg gagacataca tcagatggaa aaaatcacaa caggtgtgtc
      481 atacaccacg tcagcggtgg ggacgggata ctggttactg cagctgctgg acaaagtctc
      541 tccgtcccag tgggtggcaa taggtgtgct gggaagtctg ctgtttggcc tgctgacgta
      601 tctgacaaat ctttatttca agattaaaga agataagcgt aaggctgcga gaggtgaata
      661 atgtcgccgt cattacgcaa ggctgttgca gctgctattg gtggtggggc tgttgccata
      721 gcgtctgtgc tcatcactgg tccaggtggt aacgatggtc tggaaggtgt cagctacata
      781 ccatacaaag atatcgttgg cgtatggact gtatgtcacg gacacaccgg aaaagacatc
      841 atgcccggta aaacgtatac cgaagcagaa tgcaaagccc tcctgaataa agaccttgcc
      901 acggtcgcca gacaaattaa cccgtacatc aacgtcgata taccggaaac aacgcgcggc
      961 gctctttact cgttcgttta caacgtgggc gctggcaatt tcagaacatc gacgcttctt
     1021 cgcaaaataa accagggcga tatcaaaggc gcatgtgatc agctacggcg ctggacatac
     1081 gctggcggta agcaatggaa agggctgatg actcgccgcg agattgagcg tgaagtctgt
     1141 ttgtgggggc aacaatgagc agggtaaccg ttattatctc cgctctggtt atctgcatta
     1201 tcgcctgcca gtcatgggct gttaatcatt accgtgataa cgccatcgcc tacaaagagc
     1261 agcgcgataa agccacatcc atcatcgctg atatgcagaa gcggcaacgt gatgtagcag
     1321 aacttgacgc cagatacaca aaggagcttg ctgatgctaa tgcgactatc gaaagtctcc
     1381 gtgctgatgt ttctgctggg cgtaagcgcc tgcaagtctc cgccacctgt gcaaagtcaa
     1441 cgaccggagc cagcagcatg ggcgatggag aaagcccagg acttacatca gatgctgaac
     1501 tcaattatta ccgtctccga ggtggaatcg acaagataac cgcgcaggtt aactacctgc
     1561 aggaatacat caggacgcag tgcttaaaat aattttaatt tcactgaaat ttaacaagtg
     1621 actttcagga aaatgcctcg cagatgcggg gcatttttgt accggtattt caccgcgcac
     1681 cgcagcgcac aataaacacc gaacctgacc ctttggaatg ggcctttgag gataccagtt
     1741 agtgctggcg agcctcggtg ggctggtttc ctgtgcggca aaggttcatt tcaaagaagc
     1801 aggcaacgcc atgaatgaat taattgcgaa tcatgacttc gactttcgcc agttagttac
     1861 cgcagcagaa ggtcaaccgg taactgacac cttccagatt gccagggcat ttggtaaacg
     1921 ccatcagcat gtgattaggg ctattaaatg tttgagatgt tctgaggaat tctcgacaac
     1981 ccatttttgg gccgtcgaga aaatcaatga cttaggtatt tttgacaaga aacagattta
     2041 ctaccgcatg gactttagtg gcttcgttat gctggttatg ggatttaacg gggcaaaagc
     2101 cgatgctgtt aaagaagcct atatcaatgc gtttaactgg atgtcagcag aactccgtaa
     2161 gtacagcgaa agttatgaag cagaacgtaa cgccgtaatg ctggagtaca tgaaagagaa
     2221 ggatgtcgcc agcatgtcag gccgtctgct caatcgctgg gggagaacga aaaaacctca
     2281 attgcttgca aagctggaac gtctggagag acagggacag tttttattac cgggattcga
     2341 taaaggtatt caagcctgac acattatgcg ctgtatcgtc gccgtattcc cgcattaacc
     2401 atgaccgtag cccgacgggg aattccttct gcgtgagtgt gcgggaataa tcaaaaacga
     2461 tgcacaccgg gttttactgt gctgacagac gcagggttac cctcatagtc gcttttccgg
     2521 tgcgatggtg gaagaaaccg ggatgttcat ccatcatcac tttggattga tgtatatgct
     2581 ctcttttctg acgttagtct ccgacggcag gcttcaatga cccaggctga gaaattccca
     2641 gacccttttt gctcaagagc gatgttaatt tgttcaatca tttggttagg aaagcgg
//
LOCUS       DAZFRJ010000337         2675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000337 DAZFRJ010000000
VERSION     DAZFRJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.339"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKUN_004775"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKUN_004775"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO6662872.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKUN_004776"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKUN_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO6662873.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          664 a          660 c          646 g          705 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgcaggcat ctttc
//
LOCUS       DAZFRJ010000338         2669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000338 DAZFRJ010000000
VERSION     DAZFRJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.340"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>2669)
                     /gene="ehxA"
                     /locus_tag="ACHKUN_004777"
     CDS             complement(<1..>2669)
                     /gene="ehxA"
                     /locus_tag="ACHKUN_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO6662874.1"
                     /translation="LLIPDNYEAQGVGINELVKAADELGIEIHRTERDDTAIANQFFG
                     AAEKVVGLTERGVAIFAPQLDKLLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNF
                     TGIALSGMALDELLRKQREGEDISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLN
                     QLGSFLSSKPRLSSVGGKLQNLPDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTK
                     AAAGIELTTQVLGNVGKAVSQYILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAA
                     ADKFERAKQLESYSERFKKLNYEGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSA
                     ASSASLIGAPISMLVSALTGTISGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFE
                     NGYDARHAAFLEDSLSLLADFSRQHAVERAVAITQQHWDEKIGELAGITRNADRSQSG
                     KAYINYLENGGLLEAQPKEFTQQVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRE
                     RKQSGKYEYMTSLIVNGKDTWSVKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELG
                     DKDDVVYSGAGSSEVFAGEGHDTVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVK
                     VLQEVVKEQEVSVGKRTEKIQYRDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGG
                     KFNDIFHGADGNDYIEGNYGNDRLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNN
                     YLTGGSGNDELQAHGAYNILSGGTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVY
                     RQNYGHHTIADEGGKGDRLHLSDISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNG
                     ITFKNWFAKDASGADNHLVEVITDKDGREIKVD"
BASE COUNT          743 a          664 c          409 g          853 t
ORIGIN      
        1 tatcaacttt tatctctcga ccatctttat ctgttataac ctcaacaaga tgattatctg
       61 ctcctgaggc atctttcgca aaccagtttt taaatgttat cccattgaca tcatttgact
      121 cattaaatga aagtacacca ttaatggctt tattcatgat aagatcattt ccaactctct
      181 taaatgcgat atcatcaaag ctaatatcag ataagtgcaa acgatctcct ttacctcctt
      241 catctgcaat tgtatgatga ccatagtttt gcctataaac ataaatatca ttaccaaaac
      301 caccattcag atagtcatta ccttcccctc catccagaag atcaatacca ccaccaccat
      361 aaagtttatc atcaccagta ccacctgaca gaatattata agctccgtgt gcctgaagct
      421 catcattacc gcttcctcct gtcagataat tattaccatc ccctccactc aatttatcgt
      481 ttccactacc accaaataac tggtcgtctc cctgtcctcc ggatatataa tcatccccat
      541 catcgccgta tagtcgatca ttaccataat taccttcgat ataatcgttc ccatctgcgc
      601 catggaatat atcattaaac ttaccgcctt tgaattcatc atcatgtttt ccgccaatga
      661 gctcttcaac agaatgaaga ttatctatta catcataagg aattccaccg gttctgaatt
      721 caaaatcacg atattgtatt ttctctgttc gcttccctac tgacacctcc tgttccttaa
      781 cgacttcctg caataccttc acgtcaccat acatattttt tgaaactata tactcaccag
      841 gttttgatgc tcctgttgca tcaattgtta gtttaccaac atccgtctta ttataagata
      901 cggtatcatg accttcacca gcaaatactt ctgatgagcc tgctccagaa taaaccacat
      961 cgtctttatc tccgagttca gaaatcattc tcgcctgata gtgtttgtta ttcttttcaa
     1021 caaactgaat caattttgaa taatcatata cacctttatg attttttatg ccttttacag
     1081 accatgtatc cttaccattt acaataagag atgtcatata ttcatattta ccactctgtt
     1141 ttctttctct aacttcttct cctggggtaa atgttggtgt tataaatgtc aaaacacttg
     1201 atacattacc tgttgaaagg tctatggtcc ctttttgagg atcaaacact tgttgtgtaa
     1261 actctttcgg ttgagcctct aaaagccctc cattttccag ataattaata tatgccttac
     1321 cactctgact gcgatcagca ttacgggtta tgcctgcaag ttcaccgatc ttctcatccc
     1381 aatgttgctg ggttattgcg actgctcttt ctactgcatg ctgacgagaa aaatcagcaa
     1441 gcaaagacag agagtcttct aaaaacgcag catgtcttgc gtcatagcca ttctcaaaat
     1501 aatttttgcc atgctccttt tcccattcat tgatccgagc agcgaatttc tctgcaacgt
     1561 gctcaaacat agcctgtttt gatgcttcca gaatgccaga tatcgtaccg gttaatgcac
     1621 tcaccagcat gcttatcggg gcccctatga gggatgcact ggaggctgca ctaactcccg
     1681 cagatacaga actcaggaca gtatttattg ttgtcagggc tgcatctata gctccggttt
     1741 ctttatgaaa ggctgcgagt aaagcatccc cttcataatt caatttttta aatcgttcag
     1801 aatatgattc aagctgctta gctcgctcaa atttatctgc agcagccagg aaagaaagag
     1861 gactgatagc cagcataaca gccgatgtga tcagacccgc acttgcagct gttgtcgata
     1921 acccctgtgc cattctctga gccagaatat attgcgaaac agctttacca acatttccaa
     1981 gaacctgagt tgtcagttcg atacccgctg cagcttttgt tcctgtatgt gcgtcactgt
     2041 ttcccagaat aaagcttgct gatacagcag aaagaattcc ggagacaaca tccagcccat
     2101 cccccaggga gcccaggtct ggtaaatttt gtaatttccc accaacagaa cttaagcgag
     2161 gtttactgga taaaaatgag ccaagctggt taagctgctc agaaaatgaa tcaacggtac
     2221 tgtttatact tgatactgta tctacaagct gattaataag ttcaatacta cttttggcaa
     2281 tatcattctg acttatatcc tctccttccc gttgttttct cagcaattca tcaagagcca
     2341 tgcctgataa agcaatcccc gtaaaattct gtagtgctga gagaactgtt ccggcttttc
     2401 ccagattatt acctacattt tcagcggttc ttcctatttt actcccaact ttctgatact
     2461 tctgcagaag tttgtcaagt tgtggtgcga atattgcaac accacgttca gttaatccta
     2521 caactttttc tgctgcacca aaaaactggt ttgcaatcgc tgtatcatct cgttcagtac
     2581 ggtgtatttc tattccaagc tcatcagcag ctttgaccaa ctcattaata cccacgccct
     2641 gagcttcata attatcaggt attaataat
//
LOCUS       DAZFRJ010000339         2649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000339 DAZFRJ010000000
VERSION     DAZFRJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.341"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..460
                     /locus_tag="ACHKUN_004778"
     CDS             125..460
                     /locus_tag="ACHKUN_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO6662875.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(546..1394)
                     /locus_tag="ACHKUN_004779"
     CDS             complement(546..1394)
                     /locus_tag="ACHKUN_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6662876.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCCLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            1744..1939
                     /locus_tag="ACHKUN_004780"
                     /pseudo
     CDS             1744..1939
                     /locus_tag="ACHKUN_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1973..2224
                     /locus_tag="ACHKUN_004781"
     CDS             1973..2224
                     /locus_tag="ACHKUN_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6662877.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(2255..2500)
                     /locus_tag="ACHKUN_004782"
     CDS             complement(2255..2500)
                     /locus_tag="ACHKUN_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662878.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(2500..>2649)
                     /locus_tag="ACHKUN_004783"
     CDS             complement(2500..>2649)
                     /locus_tag="ACHKUN_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6662879.1"
                     /translation="ERLEQKGRQEGALEKALAIACQLQKMGMTPEQIKQATGLSEAEL
                     KNITH"
BASE COUNT          669 a          679 c          657 g          644 t
ORIGIN      
        1 ttatagcctg ttatttttgt gcatcatagt cttatgcaga cggcagaggc ggggcgcagc
       61 tctgccagga ggcagttttt gatgataaaa tcagacttca acatgaaacg gagaaaaagt
      121 gccaatggct tctaaaaaat tttactcaga tgatgacatc cagctggcaa aagctgcttt
      181 atcagaattg ccggacctca ccgctcaacg aaaaacattg cgtgattttc ttgatgccat
      241 tcgtgatgac atcattgtcc tggtaaggac aaagggttat acactggcag atgttcgtga
      301 cacgctcaaa aatgcagggt atgaagtagg agagaaggca cttcgtgaca ttattcggga
      361 agcagaatcg aaaaaaccat cccgccgttc ttcctctaaa acagcaccta aaaagacaga
      421 ttcaggcagg aaagatggca tcgatatgaa caacaattga tgtgacaatg tcacatcgta
      481 ttctgagatt atgatgatta cagaaaaaaa agccccggca taaataccag ggcttttctg
      541 tttaatcaga ctcgtggcgg caatacacca ggataatgcc gtgcaataat atcattcagt
      601 ttttcaacaa ggtcaggacg cttaaagaca atgtgtacgg tccctttctg gtagccacgc
      661 accgtaaaat attccccttc aaacaactca ccgatattgt cgccgttccc gagaaattca
      721 ccatactgcg ccccgatgct gttcctgaag tcaggaacgt tgcgtccctc cagcagataa
      781 aacggacgta ccaggtcatc cagtgcctcc cgtccgctgc agtccagcgt cacacgcccg
      841 ttactccagc ggtacagcag gttttcaata atgattctct tgcccagaca gcagggatta
      901 ttggttctgt aatcccatga caatttccgc cagatatcaa tcagttcctg ttcaaatgtc
      961 tgcatcttac tggcattcag atggcggaat gttgccagta cattgtccag ggtgatttca
     1021 ggacaggtgt cgctcatcag ctggctgttc cactcatcac gctggcatga actcatgaag
     1081 gtgtacatgc ccgtataagt catcaggcga tcccagattt tccggtcggc aagacgggtg
     1141 atattctttt ttgcttcttc cggtttatcc atgcatcgaa cggagtcagt cacgcagtct
     1201 tcaaacccgt agagaaaaat gtcaccggaa gcatcgagca gaatcctgcg cgctgtcgcc
     1261 aggcattcca tcgcctgcat gaacagtgca ataccttcgt tacgctgcgc gatgatgcgg
     1321 tcgatagcca tagacggaat aatatctgtc gtgccggcag tattcaggtc agcagtgggt
     1381 aaagtctgat tcatcatatt gtctcctcat cgtcacggct gccgggtcag cctggtcatt
     1441 tcccggagga gcgaagctcc ttctgccgtc cacttcacag ggggttcttc ccgcagtgga
     1501 cctttccgat gggggacgct caccacgtca cggggcagtt gtccgggggc agacgcaaaa
     1561 gtttttgacg cctgcggcaa aaagttttgt ggctggtaca tctctcccct ggacgactga
     1621 aacgggatgt ggtcgtgtat gtacccccgg aaagtccgtc tgcggagaac gggccgcagt
     1681 gcggcggcag acaggcagag acagctgcgc catcaggcgc agtggcctta cacaccgtca
     1741 ggtgtgcagg gagcgccgca tccggcgcga ctgaagaaaa acgacagcac aacaatggca
     1801 tgtggtggtc actggcgcac cactcccgca caggtgaaaa cagagaaaac agcacgacac
     1861 ctacaactaa aaagtgaaat ttcttcactt tttcactctt atttatcgcg ctgacctggt
     1921 gattatgatg ataaaataaa gtgaagtttt ttcacttttt caaaggtgtt ttatgaccgg
     1981 ctgggaactg cgaatctggc gaaaaagcat gctctggtca cgcgaaaaag cggccagaga
     2041 atttggcgtg acacagagaa catggcacgc ctgggaaaat gcggaacagg tggatgtcac
     2101 cgtctggagg accacccagg cactttcagt ccgtgatctg ctgcctcaca tgcaggggat
     2161 gcgcaaggca gatatcatca ggcgtctgga aaatgaactg ggggaaacgg cagggaatgt
     2221 gtgagcgtga ttcacagcct ggcagaatgc caggctactg ctcatcagga aacagctttt
     2281 tcagttcttc accggaaagt ccggttgcct cctgaatccg ttccggcgtc cagcccatat
     2341 cccgcagccg gcaggctgct tcccgcgcca cttcaagcgc acctttctta aaaccttcgt
     2401 cgaaaccttt ctgtataaga cgttctgcaa tcgtcatgct gtttgctctg ctcctgctga
     2461 caaaaaaacc cggcattttg ccgggctttg tggttctcat cagtgagtga tgttcttcag
     2521 ttcagcttcg gaaagtccgg tagcctgctt aatctgctcc ggcgtcatcc ccattttctg
     2581 aagctggcac gcaatagcca gggctttttc aagcgctcct tcctggcgac ctttttgctc
     2641 aagacgttc
//
LOCUS       DAZFRJ010000340         2647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000340 DAZFRJ010000000
VERSION     DAZFRJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.342"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..190
                     /locus_tag="ACHKUN_004784"
     CDS             <1..190
                     /locus_tag="ACHKUN_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO6662880.1"
                     /translation="RKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTFEPVIELYTDV
                     TWESFSALHNKINPYKEN"
     gene            192..749
                     /locus_tag="ACHKUN_004785"
     CDS             192..749
                     /locus_tag="ACHKUN_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6662881.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1040..1195
                     /locus_tag="ACHKUN_004786"
     CDS             1040..1195
                     /locus_tag="ACHKUN_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6662882.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1315..1659
                     /locus_tag="ACHKUN_004787"
     CDS             1315..1659
                     /locus_tag="ACHKUN_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO6662883.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1781..2053
                     /locus_tag="ACHKUN_004788"
     CDS             1781..2053
                     /locus_tag="ACHKUN_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662884.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2055..>2647
                     /locus_tag="ACHKUN_004789"
     CDS             2055..>2647
                     /locus_tag="ACHKUN_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6662885.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRS"
BASE COUNT          679 a          605 c          727 g          636 t
ORIGIN      
        1 acgaaagagc agaacaacag agtttctgtt tttcattctg tttatagtgt tgatgatacc
       61 gatatccccg ctattactgg tctggataat tggaaggaca tttgaaccag ttattgagct
      121 atataccgat gtgacatggg aatcattcag cgcactgcac aataaaatta atccgtataa
      181 ggaaaactga tatgagcact attaccagag aacgcgcgga gattaaatca tacatcacag
      241 gcttcctgag cgactcggcg cacgataaca agtcttcaga cagcctgctg gctaatgtgt
      301 ttcgtatcgc gctggcatca ctggaagcag agccgatagc aatggtagtg cctgatgaaa
      361 tggatttgct tacctgccat ctcgacggtg taactaaaac atatgctgat ggctggaacg
      421 cctgccgcgt cgccatgctt caggccggaa actttcggga aaataagaat tcgtcaacca
      481 acaattttcg ggaaatctcg gaaacgtcaa ccagatctcc gataactctg gatggctgga
      541 taagctgtac tgagcgaatg cctgaaaaga gccagaacgt gcttatttcg atgaatatcg
      601 atagcgaggc tgggccatta atatattccg cacgctatct cggaggcacg ttccggcgcg
      661 gaggtatagc agttagtccg ggtaatgatc ttaggcaagc aacccactgg atgtcgctac
      721 cagaaccgcc gcaggaggtg aatcaatgac ctggcctgaa gcattcacaa cggtaggaat
      781 tgcgatggcg gtggcgctgg tggtgtattc gatttgccgc tggggataaa aacggtttgc
      841 gggaaaagga tagttaagta gaattgcagc gggtgcttga ggctatctgc ctcgggcatg
      901 aacaccaacg gcagatagag aaaagcccca gttaacatta cgcgtcctgc aagacgctta
      961 acattaatct gaggctcaat ccatgctgaa cacatgtagg ttagcctctt acgtgccgaa
     1021 aggcaaggag aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat
     1081 ctgtttaacc gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtgcgaat
     1141 ccgaaccggt cagacggagg tcgctgtctt cgtagactac gaatctgaga agtaagagac
     1201 caggcggggg agtaatctcc cgccacctct gatgtgtcag gcatcctcaa cgcacccgcg
     1261 ctttaccata ctgaaaatgc tgtttgaatg ttcatctctg aaagaggact atgaatgaaa
     1321 aaggtattga ttgcagcact tatttccggt gtgtcttttg gcgcttttgc acagcagggt
     1381 ggtttccagg ggccagaagc agagcgttca acagtagcgc aggcaaaaga actgaaggat
     1441 gatgcatggg ttatccttga agggagcatc gttaaaaaag tgggtgatga acgttatgag
     1501 tttcgtgaca atagcgggac aattgtcacg gatattgatg acagcgtatg ggccgggcag
     1561 aatgtttctc cgaaagacaa agtaagaatt gagggtgaaa ttgataaaga cctgagcagt
     1621 gttgaagttg atgtaaaggc actgaaatta ttaaagtaac cgcccctgct tgttaagccc
     1681 gtcttactga cgggttttct gtttgtacat tccggcgtat tgccttacaa ttcgcgcagt
     1741 cagcctgaac aactgacacc tgctgtcacc ggagaatccg atgacacaac acataaaatc
     1801 ccacaattct gaagccgacc cggaaattaa gcaggggagg cgttttcgtg cgcctcagta
     1861 tggctggttt cactatctgt tctgtacgat cgatgaggca gatatgcttc aagaggcgta
     1921 tctgcgtcgc ggtgtccgtg tggagcggag tctgaacgct gatcgtctga cctggaccgt
     1981 ttctgtatat cttcctgttc gtgcacatct gccacggaca catgcctgct accgtcagcg
     2041 cgtctggagg taatgtgcgg gtattacttc gacctgttct ggttccggaa ctcgggctgg
     2101 tggtccttaa gcccggtcgt gaatcattgc cagtttttca tcgcggcagg gtgctggtgg
     2161 agccggaacc gaaaaacatg cgggcgctgc catctggagc ggttcctgct gttcgccagc
     2221 cgctggcgga agataaatca ctgctgccat ttttcagcga tgagcgggtg attcgtgcag
     2281 ctggcggcgc tggtgcactg tctgactggt tattacgtca cgtgaaatcc tgccagtggc
     2341 cacacggcga ttatcatcac agcgaaaccg ttattcacag ttacggtgct ggcgcaatgg
     2401 tgttgtgctg gcactgcgac aaccagctgc gcgaccagac ctccgaatca cttgagcaac
     2461 ttactcaaca aaatctgaca gcctggatga ttgacgtcat acgccatgta atgaatggca
     2521 cgcaggagcg ggaattatcg ctggctgaat tatcctggtg ggcggtccgc aatcaggtgg
     2581 cggacgcgct accggaagcg gtattacgtc gttcgctggg gttgcgtgcg gaaaaaatcc
     2641 gctcaat
//
LOCUS       DAZFRJ010000341         2645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000341 DAZFRJ010000000
VERSION     DAZFRJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.343"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            126..461
                     /locus_tag="ACHKUN_004790"
     CDS             126..461
                     /locus_tag="ACHKUN_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO6662886.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGS
                     NDGIDMNNN"
     gene            complement(546..1394)
                     /locus_tag="ACHKUN_004791"
     CDS             complement(546..1394)
                     /locus_tag="ACHKUN_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6662887.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALLPRV"
     gene            1973..2224
                     /locus_tag="ACHKUN_004792"
     CDS             1973..2224
                     /locus_tag="ACHKUN_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6662888.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(2255..2413)
                     /locus_tag="ACHKUN_004793"
     CDS             complement(2255..2413)
                     /locus_tag="ACHKUN_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662889.1"
                     /translation="MTIAERLIQKGALEVAREIACRLWNMGWPPERIQEATGLSGEEL
                     KKLFPDEQ"
     gene            complement(2472..>2645)
                     /locus_tag="ACHKUN_004794"
     CDS             complement(2472..>2645)
                     /locus_tag="ACHKUN_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6662890.1"
                     /translation="ERLEQKGRQEGRMEGRMEGALEKALAIACQLQKMGMTPEQIKQA
                     TGLSDDELKKITH"
BASE COUNT          670 a          675 c          657 g          643 t
ORIGIN      
        1 attatagcct gttatttttg tgcatcatag tcttatgcag acggcagagg cagggcgcag
       61 ccctgccagg agtcagtttt tgatgataaa atcaggcttc aacatgaaac ggagagaaag
      121 tgccaatggc ttctaaaaaa ttttactcag atgatgacat ccagctggca aaagctgctt
      181 tatcggaatt gccggacctc accgctcaac ggaaaacatt gcgtgatttt cttgatgcca
      241 ttcgtgatga cattattgtc ctggtaagga caaagggtta tacactggca gatgttcgtg
      301 acacgctcaa aaatgcaggg tatgaagtag gagagaaggc acttcgtgac attattcggg
      361 aagcagaatc gaaaaaacca tcccgccgtt cttcctctaa aacagcacct aaaaagacag
      421 attcaggcag taatgatggc atcgatatga ataacaattg atgtgacaat gtcacatcgt
      481 gttctgagat tatgatgatt acagaaaaaa agccccggca taaataccag ggcttttctg
      541 tttaatcaga ctcgtggcag caatgcacca ggataatgcc gtgcaataat atcattcagt
      601 ttttcaacaa ggtcaggacg cttaaagaca atgtgtacgg ttcctttctg gtagccacgc
      661 accgtaaaat attccccttc taacagctta ccgacattgt ctccgttccc gagaaattca
      721 ccatactgcg caccgatgct gctccggaag tcaggaacgt tgcgcccctc cagcagataa
      781 aacggacgta ccaggtcatc cagtgcctcc cgtccgctgc agtccagcgt cacacgcccg
      841 ttactccagc ggtacagcag gttttcaata atgattttct ttcccagacg gcagggatta
      901 ttggttctgt aatcccatga caatttccgg tagacatcaa tcagtccatg ttcaaatgtc
      961 tgcatcttgc tggcattcag atggcgaaaa gttgccagta cattgtccag ggtgatttca
     1021 ggacaggtgt cgctcatcag ctggctgttc cactcatcac gctgacatga actcatgaag
     1081 gtgtacatcc ccgtatccgt catcaggcgg tcccagattt tacggtcggc aagacgggtg
     1141 atattctttt tcgcttcttc cggtttatcc atgcaccgaa cggagtcggt cacgcagtct
     1201 tcaaaaccgt aaagaaaaat atcacctgac gcatcaagca gaatcctgcg cgctgtcgcc
     1261 aggcattcca tcgcctgcat gaacagtgca ataccttcgt tacgctgcgc gataatgcgg
     1321 tcgatggcca cagacggaat aatatctgtc gtgccggcag tattcaggtc agcagtgggt
     1381 aaagtctgat tcatcacatt gtctcctcat cgtcacgact gccgggtcag ccaggtcatt
     1441 tcccggagga gcgaagctcc ttctgccgtc cgggtcttcg gggatttccc cgcagtggac
     1501 ctttccgacg ggggacgctc accacgtcac ggggcagttg tccgagggca gacgcaaaag
     1561 tttttgacgc ctgcggcaaa aagttttgtg gctggtacat ctctcccctg gacgactgaa
     1621 acgggatgtg gtcgtgtatg cacccccgga aagtccgtct gcggagaacg agccacagtg
     1681 cggcggcagg caggcagaga gaactgcgta atcaggcgca gtggccttac acaccgtaag
     1741 gtgtgcaggg agcgccacat caggcgcgac cggagaaaac cgacggcaca acaatggcat
     1801 gtggtggtca ctgacacacc actcccacac aggtgaaaac agagaaaaca gcacgacatc
     1861 tgcaactaaa aaagtgaaat ttcttcattt tttcacactt atttatcgcg ctgacctggt
     1921 gattatgatg ataaaataaa gtgaagtttt ttcatttttt caaaggtgtt ttatgaccgg
     1981 ctgggaactg cgaatctggc gaaaaagcat gctctggtca cgcgaaaaag cggccagaga
     2041 atttggcgtg acgcagagaa catggcacgc ctgggaaaat gcggaacagg tggacgtcac
     2101 cgtctggagg accacccagg cactttcagt ccgtgacctg ctgcctcaca tgcaggggat
     2161 gcgtaaggca gatatcatca ggcgactgga aaatgaactg ggggaaacgg cagggaatgt
     2221 gtaagcgtga ttcacagcct ggcagaatgc caggctactg ctcatcagga aacagttttt
     2281 tcagttcttc accggaaagt ccggttgcct cctgaatccg ttccggcggc cagcccatat
     2341 tccaaagccg gcaggctatt tcccgcgcca cttcaagtgc gcctttttga ataagacgtt
     2401 ctgcaatcgt catgttgctc tgctcctgct ggcaaaaaag cccgggattt tgccgggctt
     2461 tgcggttctc atcagtgagt gattttcttc agttcgtcat cggaaagtcc ggtagcctgc
     2521 ttaatctgct ccggcgtcat ccccattttc tgaagctggc acgcaatagc cagggctttt
     2581 tcaagcgctc cttccatgcg tccttccatc ctgccttcct ggcgaccttt ttgctcaaga
     2641 cgttc
//
LOCUS       DAZFRJ010000342         2631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000342 DAZFRJ010000000
VERSION     DAZFRJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.344"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            66..1118
                     /locus_tag="ACHKUN_004795"
     CDS             66..1118
                     /locus_tag="ACHKUN_004795"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO6662891.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1194..2627
                     /locus_tag="ACHKUN_004796"
     CDS             1194..2627
                     /locus_tag="ACHKUN_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO6662892.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT          621 a          584 c          682 g          744 t
ORIGIN      
        1 gagatgcttt gtccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca
       61 ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttcc
      121 tgttagcgga acatttaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta
      181 tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatcca ctgaccagta
      241 aagtcgccat tattagccgt tccagcgatc cgcgtgctga tgttgattat ctgtttgctc
      301 aagttatcgt ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat atgctttctg
      361 gcgttggggc attcgccatt gaaaatggtt tgattgcagc gacatcaccg gtcacccgtg
      421 tgcgtatccg caacgtcaat acgggtacgt tcatcgaagc tgatgtgcaa acgccaaatg
      481 gtgttgtcga gtacgagggt agtgccagaa ttgacggcgt accgggtact gccgcaccgg
      541 ttgcgctcac tttcctgaat gccgctggaa ccaaaaccgg aaaagttttc ccgactgata
      601 atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccagtcg
      661 tcattattcc ggctgaatat ctgggaaaaa caggttatga attaccggcg gaactggatg
      721 ccgacaaagc attattagcc cgcattgaat ctatccgtct acaagcgggt aaagcaatgg
      781 gcttaggtga tgtcagtaat atggttatcc ctaaacctgt gcttatttct ccagcgcaga
      841 aaggcggggc aattaatgtg cgttatttta tgccgcattc ttgccatcgc gcgctggcga
      901 taaccggtgc tattgctatt tccagtagtt gtgcattgga aggcaccgtc acccgacaaa
      961 tcgtcccttc tgtaggatac ggcaatatca atattgaaca ccccagtggt gcgctcgacg
     1021 ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt attcggacga
     1081 ccagaaaaat attttccggt gaagtttatc ttccctgaaa aaattcgttg tcaagataag
     1141 gataatcaat aaaggacttc tgtatgagtc atacagaaag aacaggattt taaatgaata
     1201 agaaatcgtt atggaagcta attctgatat tagcgatccc atgtattatt ggttttatgc
     1261 cagctccggc aggattaagc gaactggcgt gggtgctttt tggtatttac ctggcggcca
     1321 ttgtggggct ggttatcaag cctttcccgg aacctgtcgt actgttaatt gccgttgctg
     1381 cctccatggt ggtggtcggt aacttatccg acggtgcgtt taagaccacc gccgtattaa
     1441 gcggttactc ttcaggtact acctggctgg tgttctcggc gtttacctta agcgccgcat
     1501 ttgtgaccac cggtttaggt aaacgtattg cctatctgct gattggtaaa atcggtaaca
     1561 ccacgctggg tctgggttac gttacggtat tcctcgatct ggtactggct ccggcaacac
     1621 cgtctaacac cgcgcgtgcg ggcggtattg tgttaccgat catcaacagc gtggcggtgg
     1681 ctttggggtc cgaaccggaa aaaagtccgc gtcgtgtcgg acattacctg atgatgtcca
     1741 tttacatggt caccaaaacc accagctata tgttctttac cgcaatggcg gggaacattc
     1801 tggcgctgaa aatgatcaac gacattctgc acctgcaaat tagctggggt ggatgggcac
     1861 tggccgccgg attgccgggc atcattatgc tgctggtcac cccactggtg atttatacca
     1921 tgtatccgcc agaaattaag aaggtggata ataaaaccat cgctaaagcg ggccttgccg
     1981 aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg tgtctttgtg ctggcgctac
     2041 tgggctggat tttcagtaag tctctggggg ttgatgaatc cactgtggca atcgttgtta
     2101 tggcgaccat gctgctgctg ggtatcgtta cctgggaaga cgtggttaaa aataaaggcg
     2161 gctggaatac cttaatctgg tacggcggta ttatcggatt aagctcctta ttatcgaaag
     2221 ttaaattctt cgaatggtta gctgaagtct ttaaaaataa cctggcattt gatggtcacg
     2281 gtaacgttgc tttcttcgtt attattttcc tcagcattat cgtgcgttat ttcttcgctt
     2341 ccggtagtgc ctatatcgtt gctatgttac cggtatttgc catgctggcg aacgtctccg
     2401 gcgcaccgtt aatgttaacc gcgctggcac tgttgttctc caactcctat ggcggcatgg
     2461 ttactcacta tggcggcgcg gcaggtccgg tcatctttgg cgtgggttat aacgatatta
     2521 aatcctggtg gttggtcggt gcggtactga cgatattaac cttcctggtg catatcaccc
     2581 tcggcgtgtg gtggtggaat atgctgatcg gctggaacat gctgtaaata t
//
LOCUS       DAZFRJ010000343         2629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000343 DAZFRJ010000000
VERSION     DAZFRJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.345"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1788)
                     /locus_tag="ACHKUN_004797"
     CDS             complement(<1..1788)
                     /locus_tag="ACHKUN_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6662893.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQQAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPEVLQ"
     gene            complement(1734..1955)
                     /locus_tag="ACHKUN_004798"
     CDS             complement(1734..1955)
                     /locus_tag="ACHKUN_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662894.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(2000..>2629)
                     /locus_tag="ACHKUN_004799"
     CDS             complement(2000..>2629)
                     /locus_tag="ACHKUN_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6662895.1"
                     /translation="FNIRVHAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIA
                     VLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTA
                     STGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGG
                     SFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVAVITGVNYGSASGG"
BASE COUNT          541 a          858 c          644 g          586 t
ORIGIN      
        1 ctgcagtact tccggatcgt agtcacgacc atcacgcgga acagggatat ggcttacagc
       61 ctccttcacc atctgttcaa gcatcggacg cacatcatcc ggggtgagac ttttaccgtc
      121 cgccggctgc ggaatatttg cgaccgcatc attcaccgcc tgctgcagta catccggatc
      181 ataatcacga ccatcacgcg gtaccggaat ggcccccaca gcgtcatcca ccatcgcctg
      241 cagaaccgga cgcacctcat ccaccgtcac atgcttctgt aataccgccg acagggaagt
      301 cagtttctct tcaaacgctt gtgcctgcga ggccatcttc ccctcaaatg tgcgctgtaa
      361 atccgccagc actgtggaga attcttctcc cagtgcacga ataatggaca gttcccgttc
      421 cgtcattttc tcagtatccc ccctgaacat cgctttcacc gcatcatgct ctgtttcact
      481 gattgcctta ttaccgtcag atgcgccgtc aggcagctgt gatgaaactg ttttcccggc
      541 agacgcgaac ggatcctcac gggcatcacg acgggacagc gcctccagac tgtagttctg
      601 ctgctgaaga tacagtgcat caccgccggc caggggcggc aggttctccc gtttacgggc
      661 ctcattgggc gtgagaagcg tatttttcac cgcatccccc agcgttttca tgcgccgctc
      721 actgtccatt ctcagcagcg tggtgacatc aaattctgta ctctcgtttt cccccgtttc
      781 cagcgcctca tccagtaaca gttcaatgga ctcaatcagc gtctgcaggc actgggaata
      841 atactgctgc tccagcgcct ccacgttgtc actggaaggc ggttgtccca cgccaatctt
      901 gtaggccggg acacggaaca ccgaacagac aatttcagcg gtcatcttca gttgttccac
      961 cgtctgcgca tccacaggtg aaaacgtcgt ggggttgtat tttgccccgt tgctcagaat
     1021 ggccgttttc cccgcatttt cgcctgtata cccgctgtcc cagttgctct tcagtttttt
     1081 cgcattttct tccgtaatac tgccggggat ctcaatcacc ccggacggcc tgccgccatt
     1141 tctgaaaaaa gacgtcgaat tttcctgaat atgatgcccc tgcgtggccg ccagcccggc
     1201 gacatacacc ggcggcaatc ctataagcgg atgaaaaaaa cagttaaacc ggtcgtggat
     1261 cacttcccgg gcaggcaccg tcaccgcctc cgtgatcccg cagttccggt ccggcgtgat
     1321 gcggtagaac acctcgccgt catccgccac cagaggttca acccggctcc agtccagaat
     1381 acgcagttct ttgatctgcc cccgggcatt acggattttc agcaccaccg tattgccatg
     1441 acgcagtttg gcgttcagcc acagttcaaa aaactggatg cggttctgct gggcgttggg
     1501 acgacgacag aggcgggcaa tatccccccg gcgcgtttcc ctgcgtatcc catgcgcatc
     1561 cgtctgcata agacgcagcc gcattttggc gatatcctgg gatatcagcg aaatacatgc
     1621 aaacaccgca tgaaaggaga ggacggcttc aggatcggct ttcacgccct gctgccaggc
     1681 gccggaaaag ggctcagcca ccgcctgaaa caggctggtc cagcccgcct ctcttacgtc
     1741 acgtcctgat ttctggtttt ttcgggttcg ccgtaaaagg ttccacattc gccatgctcc
     1801 gcatcacgtt tctttttctg acctgccgga cgtcgcaccg tgatgtactc cgcctttccc
     1861 aggcgaacca gcacctccgc acacggctgt gccacatcac ggatatcccc ggcccgggca
     1921 tcatgcgtgc cctgcagata tcggatcttt gccataacct gttacgggag gcgcacgcct
     1981 cccgtcctcc ttatcagact cagccgccgg acgcactgcc gtagttcact ccggtgatca
     2041 ccgccaccgc cacaccgccg tcatcagcca gataaatatc cggggcattc accagcacca
     2101 gctggtcacc cacgtactgg gagacaatca ccggcagccc ctggaaggag ccacccagca
     2161 gggtcatgtc cgggtattcc ttctgaccca gcgcattttt acgcatggac agtgccaggg
     2221 cattggtgct ggacatcagc cagaccgcac cggtgggctg caggtttgct gccacaaact
     2281 gtccaaacgc agcctctgca tccgcatccg ggttaccggt tgatgccgtg cccttcacat
     2341 catgggtgat ggacgccggg gagacatctg ccactgcggc ttttttcggg tccacaaagt
     2401 ctgtatccag acgcgccacc accgcttccg ccagcgcatt acggaccagt gcatcagcag
     2461 ccggactgga aaaacggatc aattcttccg tcagtaccgc aatggccgac accttcgcat
     2521 gactgaaggt gatggattca aaatcaaact tcgtcagggg ttttgcctta ccctcaccca
     2581 cccagccggc agcaccgccg gacacctggg cgtgcacacg gatattgaa
//
LOCUS       DAZFRJ010000344         2585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000344 DAZFRJ010000000
VERSION     DAZFRJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.346"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..850
                     /gene="gatY"
                     /locus_tag="ACHKUN_004800"
     CDS             2..850
                     /gene="gatY"
                     /locus_tag="ACHKUN_004800"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO6662896.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
     gene            879..2141
                     /gene="gatZ"
                     /locus_tag="ACHKUN_004801"
     CDS             879..2141
                     /gene="gatZ"
                     /locus_tag="ACHKUN_004801"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO6662897.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2151..>2585
                     /gene="gatA"
                     /locus_tag="ACHKUN_004802"
     CDS             2151..>2585
                     /gene="gatA"
                     /locus_tag="ACHKUN_004802"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO6662898.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYV"
BASE COUNT          667 a          596 c          677 g          645 t
ORIGIN      
        1 cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg ccaacctgca
      121 tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta cagaaaatct
      181 gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc tttttatacc aaccccgctc aggcgcgtga
      481 atttgccgag gcaaccggaa ttgattccct ggcggtcgcc atcggcacgg ctcatgggat
      541 gtatgccagc gcaccggcgc ttgatttttc tcgactggag aacattcgcc agtgggtgaa
      601 cttaccgctg gtgctgcatg gcgcgtcagg gttatcgact aaggatattc agcaaaccat
      661 caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgaccg agcaccctga agcgaccgat ccccgggatt atttgcagtc
      781 ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgcgaggg
      841 cagggcataa cgcacctgcc atttaataag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc ggcggcgatc
     1141 atctggggcc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg
     1201 agctggtaaa ggaatatgtt cgtgccggtt tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cctgcgcggg ggatcccata ccgttagcac cagaaacggt tgcggaacga gctgctgtgc
     1321 tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg agctatgtca
     1381 ttggcaccga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacacatca
     1441 cccatgttga agatgccgcc aatactttac gtacgcatca aaaggccttt attgcccgtg
     1501 ggctgacaga ggcgttaaca cgcgtgattg ccatcgtggt gcagccgggc gtggaatttg
     1561 atcacagcaa tattatccat tatcagccgc aggaagcgca gccgctggcg caatggatag
     1621 aaagcacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg acggcttatt
     1681 gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta acctttgctt
     1741 tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc cctgaaaatc
     1801 gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa tactggaaaa
     1861 aatattatcg tacgggtttt aacgattcat tactggatat tcgttacagc ctgtcggatc
     1921 gtattcgtta ttactggccg catagtcgga ttaaaaatag cgtcgaaacg atgatggtga
     1981 atcttgaagg cgtggacatc ccactgggca tgattagtca gtatcttccc aaacaatttg
     2041 aacgcattca gtccggggaa ttatcagcaa taccgcatca gctgattatg gataaaattt
     2101 atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat atgactaacc
     2161 tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc catatcggta
     2221 atgagatgct cgcgaaaggt gtggtttatg atacctggcc acaggcatta attgccagag
     2281 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgctata ccgcattgtg
     2341 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
     2401 ttcagcaagc ggatgatgat aacgacgtgg aggtgtcgtt ggttattgcg ttgattgtgg
     2461 aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
     2521 atatcgtcga gacactaatt actcttcctg aaactcagtt aaaggaatac ttcacaaagt
     2581 atgtt
//
LOCUS       DAZFRJ010000345         2583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000345 DAZFRJ010000000
VERSION     DAZFRJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.347"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..320
                     /locus_tag="ACHKUN_004803"
     CDS             <1..320
                     /locus_tag="ACHKUN_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO6662899.1"
                     /translation="RGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRTEQGANG
                     AIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPG
                     FRG"
     gene            327..1073
                     /locus_tag="ACHKUN_004804"
     CDS             327..1073
                     /locus_tag="ACHKUN_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6662900.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEAQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIEK"
     gene            1096..1857
                     /locus_tag="ACHKUN_004805"
     CDS             1096..1857
                     /locus_tag="ACHKUN_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6662901.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATVVKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIVDSSSRIVSEK"
     gene            1873..2295
                     /locus_tag="ACHKUN_004806"
     CDS             1873..2295
                     /locus_tag="ACHKUN_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6662902.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGDVYNSGYGLFPSEQARKDWKNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDRRQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            2292..>2583
                     /locus_tag="ACHKUN_004807"
     CDS             2292..>2583
                     /locus_tag="ACHKUN_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6662903.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT          694 a          563 c          770 g          556 t
ORIGIN      
        1 atcgaggtgt caaaaagttt gaccctctgg atattgcttt gccgaactgg atttctgtct
       61 cgctttggcg tgagtgggtt gaatttcgcc aggcattgcg aaaaccgatt cgaacggagc
      121 agggcgctaa cggggcgata cgggagctgg aaaaattccg ccagcagggt ttttcacctg
      181 agcaggtgat tcgacacagc atcgccaatg aataccaggg cttgttcgcg ccgaaaggtg
      241 ttcgacctga gacgttactc cgacaggtta acaccgtctc gttaccggat agtgcgatcc
      301 cgccaggctt cagggggtaa ctgaccatga aaaatattgc gacaggcgac gttcttgaac
      361 gtatccgcag actggccccg tcacatgtaa ccgcgccatt caagacggta gcggagtggc
      421 gcgagtggca actttccgaa ggccagaaac gttgtgagga gatcaaccgt cagaatcgtc
      481 agttgcgggt ggaaaaaatt ctgaatcgct ctggcatcca gccattgcac cgcaaatgct
      541 cgttttcgaa ttaccaggtg cagaacgaag cgcagcgata cgcgttgagt caggcgaaat
      601 ccatcgctga tgaactgatg accgggtgta caaattttgc gttcagcgga aaacctggta
      661 ccgggaagaa ccacttagcg gcagctatcg ggaatcgcct gctgaaagac ggtcagacag
      721 tgattgtggt taccgtggct gatgttatga gtgccctgca cgccagctat gacgatgggc
      781 agtcaggcga aaaatttttg cgggaactat gcgaagtgga tctgctggtt cttgatgaaa
      841 ttggcattca gcgcgagacg aaaaacgagc aggtggtgct gcaccagatt gttgatcgcc
      901 ggacagcgtc gatgcgcagc gtggggatgc tgacaaacct gaactatgag gccatgaaaa
      961 cattgctcgg cgagcggatt atggatcgca tgaccatgaa cggcgggcga tgggtgaatt
     1021 ttaactggga gagctggcgt ccgaatgtcg tccagccagg aattgagaag taatttttac
     1081 cgggaggaaa tttttatgga gactgttttt gacgcactga aagcaatggg aaaagccaca
     1141 tccatagaac ttgctgcgcg acttgatatc agtcgtgaag aagtgctgaa cgaactatgg
     1201 gaactgaaaa aagctggttt cgttgataaa agcgcgtaca cctggcgtgt ggctgataac
     1261 aacgttcagc aggaacagcc aatgcaggca gaactgccgg aagaaaccac cacggcaact
     1321 gtagtgaaaa tctcagagtg cgatttaacc gcgacgattg aacaacgcgg accacaaacg
     1381 gcggatgaac tggctacgct gttcggtaca acatcccgca aagttgcttc aacgctggca
     1441 atggcaatca gcaaaggacg cctgattcgc gtaaaccaga acggtaaatt tcgttactgc
     1501 atgccgggcg ataatttacc agcagagccg aaagctgcat cggtagcgga aactgatggt
     1561 aaagcctttc ctcagccagc cggtgttgcg ttaccagtac aggaagcggc aacacaggaa
     1621 gatattaaaa cagaaactgt ggcggacatt gtgcagtcgc tgccatcgtt tactgaaacg
     1681 caagcggatg acctagtttt accatcgctg catatggcaa accgcgaact gcgtcgggcg
     1741 aaaaatcatg tccagaagtg ggagcgagtc tgcgccgcgc tgcgggagct gaacaagcac
     1801 cgggatattg tccgacagat tgtcgattcc tccagtcgta ttgtgtcgga aaagtgattg
     1861 ccggaggcgc ttatggcaaa agtttttaca caggaagagc gtgaaaaaat taaggagcag
     1921 gttgttgagt tggtacgcca gagtgggcgc gagacgttaa ggcaactgga ggccaagaca
     1981 ggtgcgacaa gatatctgat gagcattctt gccagagagt tggttgccag tggcgatgta
     2041 tacaactctg gctatgggtt attcccgtct gaacaggctc gtaaggattg gaaaaacgcc
     2101 cgcaaaaaac tatccagggc aaaggtgaaa aaaacggttg tggttgatcc tgaccttatc
     2161 tggtcattac cagacggaga aatacgccgc tatgacaggc gtcagaacat aatttgtcgt
     2221 gagagcagga ggagcgaagt tatgcagcgt gtgctggcgt tctatcgggg aaactttcag
     2281 gaggtgatgg agtgagggtg agagtttata ttgccggtcc aatgacggga tatgaaaatt
     2341 tcaaccgtga ggcatttcac aaggtggaag aggaactgaa acgggaaggg cataccgtct
     2401 taaacccggc agtacttccg gacgggctga cacagccgca ctacatggat atttgcatgg
     2461 caatgattcg ttgtgtggat gcgatttaca tgctgaatgg ctggcagcgg tcagcgggcg
     2521 ctaaggcaga gctggcactg gcggagaaac tggggcatgc ggtgatttat caggaggtgg
     2581 ctc
//
LOCUS       DAZFRJ010000346         2547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000346 DAZFRJ010000000
VERSION     DAZFRJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.348"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            237..602
                     /locus_tag="ACHKUN_004808"
     CDS             237..602
                     /locus_tag="ACHKUN_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO6662904.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(703..1032)
                     /gene="tmaR"
                     /locus_tag="ACHKUN_004809"
     CDS             complement(703..1032)
                     /gene="tmaR"
                     /locus_tag="ACHKUN_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO6662905.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1204..2262)
                     /gene="yeeA"
                     /locus_tag="ACHKUN_004810"
     CDS             complement(1204..2262)
                     /gene="yeeA"
                     /locus_tag="ACHKUN_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6662906.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(2460..>2547)
                     /locus_tag="ACHKUN_004811"
     CDS             complement(2460..>2547)
                     /locus_tag="ACHKUN_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase inhibitor"
                     /protein_id="HFO6662907.1"
                     /translation="CFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT          707 a          614 c          589 g          637 t
ORIGIN      
        1 aacctactga ttgctaagga taaacaagta ttaaaggcca gactgataag accgcgacta
       61 agtgaacctg tatctgacat ggcatagctt cgctaatgtg tcatcatgat taggactaaa
      121 tctgtgaatt cctttaggaa gcacggacac aggccaatct gagtccatat gtatgattgg
      181 aatcttacac agatgacttt ttgacacggg gacttggcaa acaggggcga ccatgtatgt
      241 cagatatgac aatctcactt ccgaaagaag tcaacgggca cgttatctgt tcaaccagaa
      301 tccgacacat tctggctata cgaaagagca tacttatgca aatctatatg cttttccggc
      361 aacaagcagg aatctcagcg ctgaggcagg aattagaggt aaaaacgccc taccgggcca
      421 tgaatcaccc ggtcattgga gtagtaacca aagcagattt agccagcatg gaacacatat
      481 ctttggtgaa atgttggtta cgggaagctg gagcgcacaa cgtgttagta accagtgcgg
      541 ttaataacaa tggagttaca gaactttttg ccttgctgca tacagaagaa ggctgttgct
      601 aatttcgata ggttctcaga tgagaatgat gacggtgata cacagataag aatcaatgag
      661 caatgagggt tgccgggcaa ccctcattga ataaaacagg aattacttcg cttcgccgtt
      721 tttcatttcg cccatagctt tcagcttttt ggagatatcg cggcgttctt tggagagctc
      781 ggcatttttg atgatgtaat cgtcaacgcg atcttcatag tcacctttca tgctggcgat
      841 gatgccctgg attgcttcaa cgctcatccc gggcttgatg taatcgctca ggttgtccag
      901 caacaggacg cgcttctggt tgtcacggat ctttttctca acgtcctgaa tttcacgttg
      961 cagtttgttc ttacgacgga acagacgaac aaattccagt acgtcctgga atgaaggctt
     1021 ggtagtttcc atttttatac ccctgataat gtgagagtcg gattcgttta agcaaccgct
     1081 attcgttagg gccaacatta ctcatagctg ccgcagatga caatgctttt atcccttttc
     1141 actatcatac cctttatcct tgctgaatcg aagcagcagc aagatgattc tgaagttcag
     1201 gaattatttg cgcaaggccc ggcaaatcag actcgatagc aactcaagct gatgtgccgt
     1261 ttccatgttc agccacacat aaccatagat tggtgtttcc acaaccttca ggtcatggtg
     1321 gttattcagc aactgacgca gctcttccac agcatcgatc aatttttccg tattggcaaa
     1381 aaccggctgc ggattacctt cgtacagcgc atgaacaagg ctcagcagta tttgctgcat
     1441 catgtgctgg gtatcacgaa gtttttgcgc gttcaataac acgaaatggc tggggcgcgt
     1501 ggcccagtat gcattgattt gcaactccag catacaaacc agattgcggt taatggtctg
     1561 gataccttca tatatcgatt ttggaatacg agtttctttg ctggcgggcg caatcagccc
     1621 acgcattttc acggcatcgg tcaggagttt ttgtagatgg ctttccagac gtgggcgttc
     1681 gagtaagttc ggtgagaatg cagattgata aacccgatta tactcggtca gacttttcgc
     1741 cagttgaatg cgccagtgga tgaacgcccg ttgtggccag ataccggtaa acaacattgc
     1801 cagtaaagag ccgaggatca catcgccgct tcgccataac gccgtatcaa tttcacctgt
     1861 cggggaaccc acaacaattg ccagcgtcac cccaatcaat aatccttgat acggtttctt
     1921 gcccagcgcc agccaaccgc aaagaaacat agccgccgcg caccagacta acatcagcgg
     1981 taacgaaatt aactccagtt gcagagcgat aagacctaaa atcgaaccca acaccgtacc
     2041 gccaatacgt tcgaaggcgc gagggacaac gttaccccag aacgagattg gccccataat
     2101 caccaccatg gtgaccagcg gccaggtgct ttccgggata gtaaacaggc ggataatgag
     2161 aaaagtgagc aggaatgcca gcgcgacccg agtaccatgc acaatgcggt aatggcggta
     2221 tacccggatt tcaaacgggc ttaatgactt atcggcacgc acgcagaaac tccgtaatga
     2281 agattaaaag tgctcataac tcatctgtcg gccagaaggc ccgcctctga attatccgac
     2341 ggacaaaccg ggaacagtgt aatttcccgg caggagcgaa aaaattgaaa ggcgcatctt
     2401 actcttttcg ctttcaaaaa aagagtggtc atcgcgttaa cacaccgccc tgagatgaat
     2461 tagtgatgtt ttggctgcac cgcaacatac atttcgatat cccagtaccc atcttccgcg
     2521 ccattgttca aataaacctc gaagcag
//
LOCUS       DAZFRJ010000347         2480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000347 DAZFRJ010000000
VERSION     DAZFRJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.349"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..83
                     /locus_tag="ACHKUN_004812"
     CDS             <1..83
                     /locus_tag="ACHKUN_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HFO6662908.1"
                     /translation="QLIIAVVAVGKRERSDVYNLASERMR"
     gene            88..496
                     /locus_tag="ACHKUN_004813"
                     /pseudo
     CDS             88..496
                     /locus_tag="ACHKUN_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1143..2120
                     /locus_tag="ACHKUN_004814"
     CDS             1143..2120
                     /locus_tag="ACHKUN_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO6662909.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            2304..>2480
                     /locus_tag="ACHKUN_004815"
     CDS             2304..>2480
                     /locus_tag="ACHKUN_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6662910.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          677 a          578 c          599 g          626 t
ORIGIN      
        1 aacaattaat tatcgctgtt gtagctgtgg gtaaacgtga gcgtagtgac gtttataatc
       61 ttgccagtga aagaatgaga taattaacgg cggattatcg gttcagccct ctcattaacc
      121 gctgatgctg agttggtgtg tatggctttg cgtaatgcac tggaaacacg accgcgtgat
      181 ggtcgcctgc tgtttcattc agatcaaggt gttcagtata aaagcaataa atacagaaag
      241 ttactctggc gttatggggt aatgcaaagt atgagtcgca gagggaattg tctggataat
      301 tcgccgatgg aaaaagtctt tcgcagcctt aaaagtgaat ggcttccgaa aggtggttat
      361 ggtgattgcc atgcagtacg cgaataaatc agtggataaa tgcttattac aacgtttatc
      421 gacctcatac aaacaatggt ggtttatcgc cttgccgcat gaagaaaaat ggaaacaggt
      481 tattccggtg tcctgatttt atgatccact acacattcct gacgaacgcc actgcataaa
      541 tcacattgca catcctcaga gagtacgcag tataggaggg gtatatatac attatcggta
      601 tatcaggcga cttctgtctg tatgttgtcg gtgctcgccg cagaccggtg accgggcgaa
      661 gcccgcgaac cggcgaggaa gcgcaatgta tctgtagtta cagtgagcag atacgccctg
      721 aagcttcctc ataaaacagt tgtgtaaact gttttcccgg aatgatttat ccacatatcc
      781 ggcggcccga tccagtaaac agatccataa atgatcaaga aatgatccat taaagatccc
      841 catatcgctg caaaccttgt cactcatgct ccgggaccac gatcacataa gctgtggcat
      901 gtttctgata aactgtagca ggatcatgat aagctgtatt cagtaatgta tatactgtag
      961 taagttaatg acataaatta tggtcagtac gccaggctca gctgttaaat acagactgca
     1021 ggtttttctt cagtcagttg acggggttct gacactcaat tttctgttta ttcttttact
     1081 gtccacaggc aggaggcttt ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg
     1141 aggtggataa gtcgtccggt gagctggtga cactgacacc aaacaataac aacaccgtac
     1201 aacctgtggc gctgatgcgt ctgggcgttt ttgtaccgac ccttaaatca ctgaagaaca
     1261 gtaaaaaaaa tacactgtca cgtactgatg ccacggaaga gctgacacgt ctttccctgg
     1321 cccgtgctga gggatttgat aaggttgaga tcaccggccc ccgcctggat atggataacg
     1381 atttcaagac ctgggtggga atcattcatt cctttgcccg ccataacgta attggtgaca
     1441 aagttgaact gccttttgtc gagtttgcaa aactgtgtgg tataccttca agccagtcat
     1501 cccgcaggct gcgtgagcgc atcagccctt ccctgaaacg cattgccggt accgtgatct
     1561 ccttttcccg caccgatgag aagcacaccc gggaatatat cacccatctg gtacagtcag
     1621 cctactacga tactgaacgg gatattgttc agttacaggc tgatccccgc ctttttgaac
     1681 tgtaccagtt tgaccggaaa gtgctgctgc agctcaaggc catcaatgcc ctgaagcgac
     1741 gggagtccgc ccaggcactt tacaccttta tagagagcct gccccgggat ccggcaccga
     1801 tatcgctggc gcggctgcgt gcccgcctca atctgaagtc tcctgtattt tcccagaacc
     1861 agacggtcag acgggcaatg gagcagttgc gcgagattgg atatcttgat tacacggaga
     1921 tccagcgggg gcggacaaaa ttcttctgta ttcactaccg gcgtccccgg ttaaaagcgc
     1981 cgaatgatga gagtaaggaa aatccgttgc caccttcacc tgcggaaaaa gtcagtccgg
     2041 agatggcgga gaaacttgcc ctgcttgaaa aactgggcat cacgctggat gacctggaaa
     2101 aactcttcaa atcccgctga acataaactg tagtcagtga agagtgctcc tttactgact
     2161 acagcttata ttatcaggtg cagtgagtgg tctgctcact gcagtttata tgtaagcgta
     2221 cagcctgaac cgtctggtca gaatctgacg aattagacaa agtggtgtcc accaaataag
     2281 tagtgggaac caaagtgtca gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct
     2341 gccctaatta tcctcccgaa tttaaacagc agctcgttgc tgcctcctgt gaacccggga
     2401 tatccatctc aaaacttgct cttgaaaatg gcattaacgc caatctgttg ttcaaatggc
     2461 gacaacaatg gcgcgaggga
//
LOCUS       DAZFRJ010000348         2458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000348 DAZFRJ010000000
VERSION     DAZFRJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.350"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..153
                     /gene="ymgC"
                     /locus_tag="ACHKUN_004816"
     CDS             <1..153
                     /gene="ymgC"
                     /locus_tag="ACHKUN_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO6662911.1"
                     /translation="HIIDQALRLRLKDETSVILSDLYLQILQYIEMHKTTLTDIIIND
                     RESVLS"
     gene            485..2008
                     /gene="pdeG"
                     /locus_tag="ACHKUN_004817"
     CDS             485..2008
                     /gene="pdeG"
                     /locus_tag="ACHKUN_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6662912.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2140..2358
                     /gene="ymgF"
                     /locus_tag="ACHKUN_004818"
     CDS             2140..2358
                     /gene="ymgF"
                     /locus_tag="ACHKUN_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO6662913.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          660 a          518 c          533 g          747 t
ORIGIN      
        1 catattattg atcaggcttt acgcttgcgt ttaaaagatg aaaccagtgt gattctttct
       61 gatctctatc tgcaaatatt gcagtacatt gaaatgcata agactacact aacggatatc
      121 attattaatg acagggaatc cgtgctctct tagaacttga gtaaggtttt agaataaaca
      181 tcagctgtat caccatgctg atgcaaagtg agcatttcag gcgttatgct ttcttattat
      241 gtccgcaata tcaggtgtca agaatggaga gttctcgctc tccattcttg acgcctgata
      301 tcccgcctaa cttattttgt actgcctaca ttatgctcaa taattaatgg gttagatcaa
      361 gatggagaaa attttatctt cggcgtggat atttcttatg ctctctacca tgctctctgt
      421 agatgccagc gcgagcgaat gaatggcatt ttcatgtatt aaaccctttg gccgggacaa
      481 gcaaatgcgc aatacactca tacccatcct cgttgcgata tgcttgttta ttaccggtgt
      541 cgctattttg aacatccagc tctggtattc cgcaaaagca gagtacctgg cgggagcgag
      601 atatgccgcc aacaatatca atcatatact tgaagaagcg tcacaagcga ctcaaacagc
      661 ggttaacatt gccgggaagg aatgcaacct cgaggagcaa tatcagcttg gcactgaagc
      721 agctctgaaa cctcacctgc gcacaatcat cattctcaaa cagggaatag tctggtgtac
      781 atccctgcct gggaatcggg tcctgttgtc tcgtattcct gttttcccgg acagtaattt
      841 actgttggct ccagcaatcg acaccgttaa tagattacct atcctgctct atcagaacca
      901 atttgcagat acgcgcattt tggttacgat aagcgatcag catattcgtg gggcacttaa
      961 tgtacccttg aaaggggtaa ggtatgtatt acgcgtggcg gatgacatta ttggaccaac
     1021 gggtgatgtg atgacgctta atggacatta tccctatacc gagaaggttc actccacaaa
     1081 atatcatttc actattatct ttaacccgcc gccactcttt agcttctaca gacttatcga
     1141 taaaggcttt gggattttaa tatttattct gttaatcgcc tgcgccgctg ccttcctgct
     1201 tgatagatat ttcaataaaa gcgcaacgcc tgaagagatc ctgcgacggg caataaataa
     1261 tggggagatc gtcccttttt accaacctgt ggtaaatggt cgggaaggga cattgcgggg
     1321 agttgaggtg ttagcccgct ggaaacaacc tcacggtgga tatatatcac ccgcggcatt
     1381 tattccactt gctgaaaaat ccggattaat cgttccgctt acgcaaagcc tgatgaatca
     1441 ggttgccaga cagatgaacg ctatcgcgag taaattgccg gaaggttttc atattggaat
     1501 taattttagc gcttcgcata ttatttcgcc gacgtttgtc gacgagtgtt taaatttccg
     1561 tgacagtttt acccgccgcg atttaaacct tgttctggaa gtcaccgagc gtgagccatt
     1621 gaatgttgat gaaagtctgg ttcagcggtt gaacattctg catgaaaatg gttttgtcat
     1681 cgcgctggat gatttcggta ctggctactc agggctttct tatcttcatg acctgcatat
     1741 tgattatatc aaaattgatc acagtttcgt tggccgcgta aacgcagacc cagaatcaac
     1801 ccgaattctg gattgtgtat tggatctggc gcgtaaactt tcgatcagta tcgtcgctga
     1861 aggtgtcgaa acgaatgaac aacttgacta tctgaaccaa aataatatca catttcagca
     1921 gggttattat ttctataaac ctgttacata catcgacctg gtcaaaatta tcctttctaa
     1981 accgaaggtg aaggttgtgg ttgagtgaaa attgatcagt aaggccatag tgcggtgtaa
     2041 ttatagacag ctaattagct cgttgcctct tgttactatt gttcattatt ttgtttgcta
     2101 taattgtttg aaagttttga caggattgcc attagtagca tgaacaatag taataatctg
     2161 gattatttca ctctctatat catcttttcc attgcattta tgctgatcac cctcctggtc
     2221 atccttattg caaaacccag taccgggctg ggagaagtgc ttgtgacgat aaatttgctt
     2281 aatgcccttg tttggctggc gatcaatctg gttaatcgat taagggaaag actcgtcagc
     2341 cacagggatc agcaataatc tttcagtttc tcactgtcag tatgcggctg aatgggttgc
     2401 tggcagtgaa tgcctggatc attgaagtaa aggaattatt gcgcaaatag ttgtcaac
//
LOCUS       DAZFRJ010000349         2453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000349 DAZFRJ010000000
VERSION     DAZFRJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.351"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(16..312)
                     /locus_tag="ACHKUN_004819"
     CDS             complement(16..312)
                     /locus_tag="ACHKUN_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO6662914.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            complement(451..684)
                     /locus_tag="ACHKUN_004820"
     CDS             complement(451..684)
                     /locus_tag="ACHKUN_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6662915.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            798..1502
                     /locus_tag="ACHKUN_004821"
     CDS             798..1502
                     /locus_tag="ACHKUN_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6662916.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1771..2133
                     /locus_tag="ACHKUN_004822"
     CDS             1771..2133
                     /locus_tag="ACHKUN_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662917.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            2149..2277
                     /locus_tag="ACHKUN_004823"
     CDS             2149..2277
                     /locus_tag="ACHKUN_004823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662918.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
BASE COUNT          714 a          506 c          541 g          692 t
ORIGIN      
        1 gatccagtaa ttccctcaga attgcatatc aatttgctta gagtccccgg cggccaccgg
       61 ggattttttc tttgtgattt catcaagcgc atacttaaaa gccctgctaa tcggactgat
      121 gtctgatgcc attccgaaag cacacaagac cgaagcaata aatctccagt ccgttctgct
      181 tatcttcgat tcatgacagc caatcatctt tgccagaccg cgctgggtaa gcgttgacag
      241 gttgataagt aaatctgttt ctgcgcgatc aacatcacgc tgtgatagtt tgctgtaact
      301 tgtttgttcc atttcttaag atttccaata gtgaatagct agttgaaagg tatgcgtgga
      361 aacgcatatg gccttagttg gtcagatatc ttggaactcg cttttcagcg acgtaggacg
      421 aatgtccgtt gttacaaaga gcggggttac ttatgctgct gatgctctac gcgatacgaa
      481 caccagattt tcctttttca caggtttata acccgtgaaa ttacgagtgg cttcttcgat
      541 tgcattcgct ttatctgggg aagctcttcg aaacccatat acaatctgat caagatagcc
      601 aactgaagta ttcgctaatg cggcgagtcg cttccattcc tcactagaag cctcttttcg
      661 ccagcgtagt agttcattac tcattagtgc ctccgtttat cacacagagc gactttacca
      721 ttttgataaa taaaacgcaa tgaagattta tcaaaatgca tatttatcca tttgctaaat
      781 agagggagaa tcgtgggatg gaaaacaaag atattcgcaa atcgaatctg gcgtttttgc
      841 tagatgagca taaaaaaatc gcgggtaaca ctaatgcaag ctttgccgat aagcttggag
      901 ttagcccttc tcaactcacg caagtctccg gtgaaaaaag cactcgaaac ataggggata
      961 aactagcaag aaaattcgaa gccgcacttg ggttacctaa tgggtggctt gatttggtac
     1021 atgatgtaac accaattgca tcatgctcag attctttaac ttttgtcggt caggtaagaa
     1081 aagggttagt gcgcgtggtt ggtgaggcaa ttcttggtgt tgatggtgcc atcgagatga
     1141 ccgaagagcg cgatgggtgg ctcaatattt atagcgatga tccagatgcc tttggtcttc
     1201 gtgtgaaagg agacagcatg tggcccagaa taaaatcagg agaatatgta ctcattgagc
     1261 ctaacaccaa agtattcccg ggggatgagg tgtttgtcag aactgttgaa ggacacaaca
     1321 tgatcaaagt tcttggctat gacagagatg gagaatacca atttacaagc atcaaccagg
     1381 accacaggcc aataacgttg ccttatcatc aagtagcaaa ggtggagtat gtggctggta
     1441 ttctgaagca atctcgccat ctggatgaca tcgaggcaag ggagtggctg aaaagttcgt
     1501 gacttcatcg tcacatagct ggtaaccagt ggcctgaaga gacgtttggg tgatgtgata
     1561 gagatcatta ttcaattcga ttgagaaaaa tcatcataag gtttatatcc atttattaga
     1621 ttttatgtgt ataaatagtt aaaaatgcac gatgtagaga tatttgtgct taacatcaat
     1681 taatcattgc taaatcgtgc ttattgttca gaaatcgccc aagtcatatt gaaaatctgt
     1741 tggcgaacac ctatataagg agtatagtta gtgacccaaa acgtattgcc aatcaacaag
     1801 agcttgcatg accgagctgt tgatgaattt aatcgtctgc atggaacaat gattggcgag
     1861 attagtgcaa tgctgaaaac agctaaagta gcgccattgg tggatcttcg taagaaagat
     1921 cctacctttt cgaacgttgt cgcagaatta cggacattca gagacgtatg taatgctctg
     1981 ctcccatact ttcgtgtaga taagaccagc gaaatcgctg tcatcgataa gttacttata
     2041 ctggctaatg acctggctca agccatcgat gctgacgatc ctgacgcatt gtgcgcagca
     2101 atagcagccc ttgatgttga gccttacatt taaacgaggg gaaaaaaaat gactaaagcg
     2161 tttgactata ctgccgttag taagcttttg gctgaaatgc gcggatgtgt agagcgcgtt
     2221 caaaatctac gccgcgactt tgaagctcac atcaatcatt cacaaaaagc ggcttgatac
     2281 ttaagttaga tgacagaacc cggcctcagc gccgggtttt ctttgcctca cgatccccac
     2341 acctaataac accttaacca attgtattta ttgaaaaatt gatagataca actttctaaa
     2401 ccacgaaatt ctgatccccc tcaagtctct tcattccttc tgataaattc ccg
//
LOCUS       DAZFRJ010000350         2432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000350 DAZFRJ010000000
VERSION     DAZFRJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.352"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1358
                     /gene="espK"
                     /locus_tag="ACHKUN_004824"
     CDS             <1..1358
                     /gene="espK"
                     /locus_tag="ACHKUN_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO6662919.1"
                     /translation="PTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYIKD
                     CDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKNLH
                     IELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSLSI
                     NRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIVAE
                     GVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVNSI
                     KLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNHNN
                     YNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEGYG
                     QSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTYKY
                     S"
     gene            complement(1735..>2432)
                     /locus_tag="ACHKUN_004825"
     CDS             complement(1735..>2432)
                     /locus_tag="ACHKUN_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO6662920.1"
                     /translation="TKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGVI
                     RYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIK
                     EGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRI
                     LPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTF
                     GMKENISMVNASK"
BASE COUNT          806 a          528 c          435 g          663 t
ORIGIN      
        1 ttcctacatc gcaattacga ccgtccggga cattctactc caattccgca gaaacatcag
       61 caggcatcaa aagcgaaatc acaccaactc agatagaaga agcgcgggcc attggtcgtt
      121 tatatatcaa agattgtgat attgagtatc tgccacagtt accaaacgaa ataacatcag
      181 ttacaatcga aaattgcaat aacctgacca cccttacagg attaccggtt aatacacaaa
      241 acctgtccgt cattaactgt gaaaaattac aaatcacaga cataccatcg acagtaaaaa
      301 atctacatat tgaattaact gattcaccat ttatacacat catatctgaa ggcatcgaat
      361 gcctgacggt ttgccactgc catatatctg gagtgccaga gagtgtccgt catcttgaga
      421 tcagaggtta cgccacagac ggcataaaaa atgttccaaa cgggttatta tctctcagca
      481 tcaatcgcta caccccagaa aatcaggcca gaattgacag cctgatatca ccgtcactac
      541 agacgctatc tctgactgga tgtagcaata ttatactgcc ggagaaactc ccggagagtc
      601 tgagatctgt aaccattcat gtggagcaga aaactacatg gaacatcgta gctgaagggg
      661 ttcctgatgg actggatctt gatttacaaa atgtactact ctctccgaat gtaattaaag
      721 aaaaaaacat cacttttcag ggcaacgcac tggatgctgc cttgcatttt cgtccgggag
      781 acattgtcta tgggctatcc tctcccagag aaaaacttgt caacagcatc aaactggtta
      841 acgacttttc caaaaaagat atcgtaattc agaatacatt aacaaacgca gtatgggacg
      901 ccagaacccc ccgtaaatat aagcaagact cgcttatcaa aagagcatta aatgaacatg
      961 aaagaggaac aaaatttaaa cagtgcttaa aaaatcacaa taattataat gtcatcatgg
     1021 ccgacctttc catatacaat cgagacaagt tatgggcaaa aacaagcaag gcaggcctag
     1081 agtttcaaac attaattcgc aacagaacgg ttatgttttg tgcggatggg cttgtcaatt
     1141 cactcaaact catagctaac aagtcagagg gctatggtca gagtattacc gccagcgaat
     1201 tacgatggat ttatcgtaat aaagacaaca gccaaataat gaaaaacata aaattttatc
     1261 tgcatggcaa agagatacca gcagaaagaa tattagatac accagagtgg aaagactacc
     1321 gtccaaaata ctccggttcc acatataaat attcttaatg atagcaaaaa atatattttc
     1381 gatataatca atgttatgat taaagagtat ttcatcaggg caggtaaaaa cagagtaaat
     1441 cagcaaaaga agctgatctt cagcgatact gacactaact gacggtttaa gcggtcgtat
     1501 gaagcagcag ctttccgacg gactgccatg cggatcgttt accttttggg ctattccgcc
     1561 cgtcatcaag cggctcacga gtactgagtt tatcaggatg atattgctga caatggtaat
     1621 tcgttgaccg atgtatactt cactatatat cggtcaacac cgtactgccg tgcaacatct
     1681 caaagatgta gcatcgtttt taccagagga ggcaggacat agattgtaaa aaaactattt
     1741 cgatgcattt accattgaga tattttcctt cataccaaaa gttctatcaa ttatcgcaag
     1801 tggttcaaca ggtgcggtct tatctataac ccaaccactc tctgtaacac ttcctcgtgt
     1861 gatatatttt gcctttactt taacaactat tagataatgc tcacgactaa accgatcagc
     1921 aacgctaaga tccgatgtgt attcaggcaa tattcttcca atacgtacct gctgttgtgc
     1981 catatattca tctggtgctt ttacatttgg atttgaatca ggaagccctc cagcagtttt
     2041 atattcaacg atattttcag cttgaatacc ggttgtgccg cgacacaata tgatgtctga
     2101 atcggacagt tctatgtgcc cctccttaat tttcgaataa atttgtttaa tactatgttc
     2161 tggagttggt actatttcag aagataaagc aggcaacgtg tcttcaagca ccggagaatc
     2221 atcgtatata tccaacaaat cctcatcata cccaatatct ccttctctta actgagaata
     2281 cggtttatct atgtatctaa taactccccc accatgaaga ccttctgatt tataaaatgt
     2341 accatcagcg tcacgaaaga aataaacatt aacctgtttg ttatttatag aatacctacc
     2401 tgaatataat tccgaatttg gctccttagt ta
//
LOCUS       DAZFRJ010000351         2423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000351 DAZFRJ010000000
VERSION     DAZFRJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.353"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..238)
                     /locus_tag="ACHKUN_004826"
     CDS             complement(<1..238)
                     /locus_tag="ACHKUN_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662921.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNR"
     gene            complement(247..339)
                     /locus_tag="ACHKUN_004827"
                     /pseudo
     CDS             complement(247..339)
                     /locus_tag="ACHKUN_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(372..710)
                     /locus_tag="ACHKUN_004828"
     CDS             complement(372..710)
                     /locus_tag="ACHKUN_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6662922.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(713..1018)
                     /locus_tag="ACHKUN_004829"
     CDS             complement(713..1018)
                     /locus_tag="ACHKUN_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO6662923.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1333..1983)
                     /locus_tag="ACHKUN_004830"
     CDS             complement(1333..1983)
                     /locus_tag="ACHKUN_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO6662924.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            2064..2249
                     /locus_tag="ACHKUN_004831"
     CDS             2064..2249
                     /locus_tag="ACHKUN_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6662925.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2359..>2423
                     /locus_tag="ACHKUN_004832"
     CDS             2359..>2423
                     /locus_tag="ACHKUN_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662926.1"
                     /translation="MAQASYSKLTQREIDRAETDLL"
BASE COUNT          621 a          551 c          478 g          773 t
ORIGIN      
        1 atctatttct cctttgtttc cattcctgta accactgatt tggtgctggt ttaaaattaa
       61 caatccaatg cgcaggaacc aaccatgcat aatgctctgt ctgatgaaaa gctatatatt
      121 gaagtgcgaa tatttttatc ccatcttctt caactgtcgc ctggaatctc cagaaaacag
      181 gcattccatc atgttcagtt tctgattcag gaaaaggtac gctccatgat tttgtcatat
      241 ctcacctcaa ataagtggtt tgctgcctaa tttcattttc tggcgaccaa cacaagtcac
      301 acccatttca ctgcgtggct tgctgtaata aattaggtta gttcagccaa taaaaaaccc
      361 accgaagtgg gctatgacca ttttttattt ggatttcgtt ggtgagcgtg attaacaact
      421 ctgtgcatta catcctcata tttttcatct tcaatttttt cgacatcgcg aggaaatggt
      481 gttgctaatg ctttgtcaac tttgtccatt gggtcttcat taatcttata ttcaggaccg
      541 tcatctatag cattaaatcc aggtgttaca ccgtttttta atgcatatgc tatcctcttt
      601 tcccatctcg ctattctcct cctgtctcga gatgtaagac ctctatcaga tacttttctg
      661 ttttgtccgc ggtcaggatt aacataaata gtctttttca ccataagcat actcaataag
      721 caccgtacgg tagtttactg tacaatttta ttttttggac tgcatgtatt ttgtttccta
      781 atgggtttga atccttgtaa taaatacttc tattttttcg aacaacttct tctttcttct
      841 tgcagcaaag gcttcctagt gatgctgctt tgtctgctct gacgcaacca gagagcttta
      901 gcgcaatttt tcgcgccagt gcttcattac tgcgtcgctc ggcaataagt tctgctctgc
      961 gagctttgta gcggcttttt gccgtacctt tggattcttt ccagacaatg gttaccatga
     1021 tggtctcctt taagtggctt tggcgcatga cgcgtcgagg tgcttatctt ctcgatcgct
     1081 gtcttgcagc tgcaattcgc gccatcccca aaaccactca agttctggtc tcaacggtta
     1141 ggttgagagt tcgtcgatgt taaagagcct gccaatctgt tccgtttggc ttccagcctc
     1201 ctgctgacgg cttaaaatta agacctctta attttttggt caagtgcatt tttgaagaaa
     1261 acttaatttt atgggcgcga atttagtttg tctttgattt ttaacgggaa ataaaaaagg
     1321 ggcgaaagcc ccttaaggaa ggtttgctag cttggcatca acgacaacgc caatgatttt
     1381 acagttccca ttgatttcaa tcattgggta ttgtggattg agtggtttca ggaattttct
     1441 accggcatca ataactaact ttttgaatgt cgcctcgttt tctccttcaa gtttggcgac
     1501 taccagcttt ccattacgtg gttcgacttc tgggtcgacg agaataatca tcccttcagg
     1561 aatactcagt cctgccgggg cagtcattga atcaccttta acgtcgagcc aaaaagagtc
     1621 ttcagaacaa tctaccgttg tgtcgtacca gttatctatt gcacgcctat gatatggctc
     1681 tacagcttcc atccaacatc ctgcgcttac ccaactaatt agaggatacg aacctcttgg
     1741 atcatgcctg ctgtgatagg caatgtttga aagactatcc tctcctttca acaggtaatc
     1801 aggggagcac tgcaaagcct tggctaaggc caataggttt tcgccattgg gctcagtttc
     1861 agatcgctcc cattgggaaa tagcaacatt agacacgcca accatcttgc caagggcagc
     1921 ctgcctaatc ttgagttctt ttctgcgagc gcgaatacgc tcacccatca gttgtgtatt
     1981 catagttaag acatcttaaa taaacttgac ttaagattcc tttggtggat aatttaagtg
     2041 ttctttaatt tcggagcgag tctatgtaca aaaaagatgt tattgaccac ttcggaaccc
     2101 agcgtgctgt tgctaaagca ctaggcatta gcgatgcagc agtctctcag tggaaagaag
     2161 ttatcccaga gaaagacgcc tatcgattgg aaatcgttac agctggcgcc ctgaagtatc
     2221 aagaaagtgc ttaccgccaa gcggcataag taaattgctc tttaacagtt ctggcctttc
     2281 acctctaacc gggtgagcaa acatcagcgg caaatccact gggtgtgccg ctatatatca
     2341 atataggaaa attaacaaat ggcacaagca agttacagca agctaacaca gcgagaaatt
     2401 gatcgcgcag aaacagattt act
//
LOCUS       DAZFRJ010000352         2421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000352 DAZFRJ010000000
VERSION     DAZFRJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.354"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..472)
                     /locus_tag="ACHKUN_004833"
     CDS             complement(<1..472)
                     /locus_tag="ACHKUN_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6662927.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTS"
     gene            complement(543..>2421)
                     /locus_tag="ACHKUN_004834"
     CDS             complement(543..>2421)
                     /locus_tag="ACHKUN_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6662928.1"
                     /translation="PDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTF
                     AITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWD
                     TPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQ
                     QGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQ
                     VETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLD
                     FFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQ
                     QIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAM
                     INPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADI
                     SGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNG
                     TVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIAS
                     RETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISI
                     R"
BASE COUNT          515 a          707 c          593 g          606 t
ORIGIN      
        1 gagacgtgtt gctgtggcga ccgtcatcac ttccggtcag cacgtcgtgc gttttcccct
       61 tgttgtcagt tacgcggaga taatccccgg agaaagtcga cacacggctg taagccacgc
      121 ccgccatcgc atacgcgctg aaccattcat tcacgcgcac agacggcccc gccattacgc
      181 tgaaccagcg gttacgaacg gaatcttcat gccagcgggt atcgctgtaa tgggtcagct
      241 ggcgattctt gtctcctgca tagctgaacg acgtcaccat ccccagtgtg tccgtaaact
      301 cataacggta tttcacgtta atcccgttca gttcatcgct gccaggaacg ttcgtcgaga
      361 catgaagata ccccgcgctc agcgtggact gatgttcaga cgcccatgca ggcgcaccgg
      421 atacggccag acagatggct gcggacaaaa tggcggcata aagtttacgc ataattacct
      481 ctcgcttttc tgcaataaaa aaggcgtcat tcctgacgcc ctttattggg gttataaata
      541 tttcaacgaa tactgatgcc ggaagcagct tttttggtca caatcaccgt acagtcggtg
      601 atattgcctg ccccctgatt gcctttctgg aaaatcttaa actccagagt gacgctacca
      661 ccaccactag gcatatcaat aactgcactg taactaccgg gaatggcccc tttagtttct
      721 ctggatgcga ttaatacgcc gtttttgcga acttcaaaac cataccccgt gtatcgcgtg
      781 cctcctgggt tatttccgct ccctggatcg tcatacgcta taccgttaaa aataatgggc
      841 ggaataataa tctggcggtc aaagttatga tcatcgctga tggtgactgt aaccgtaccg
      901 tttggtgttt ccgtgttacc ccacgtaccg acttttttcg ggaaggcttt tgatacagct
      961 ttaacgaaat cccctctgac ctgggtcgcc tccagcatgc ccttaatcgt acagttctgg
     1021 ttaatcgtga cattgttgag cgctcctgag ttcgcattca cactgccact gatatccgca
     1081 tttttcgccg tcagtcgccc gtctggtgtc agggaaaatg ccggaggatt accgccgctg
     1141 gtaatggtgg gagccgtcag atatttcagg aacacttcat tcatgaatat ctgatcgccc
     1201 tgaccaacaa acatcggctt tgtgttgcca ttcgcaggat taatcatcgc aatcctgtct
     1261 gctgccagca gcacctgact ctgcatgccg tcgggggtgt tctcaatacc ggcaccgata
     1321 cccgcaatat aaaggcgtcc gtcctgcatc tgctgcagct tcactgccca catgctgttc
     1381 aggttattat ttgtatcaac ctgaaccttc tgtatctgct ggatcgctgc actctggtct
     1441 tccagtttct tattgacggt ctgtgttatt tcattgctga catccgttat ggacgtcctg
     1501 atttcagtca ggtcaggcgc aagctgaccg ttatcaatct gcgtccacag ctcctgagcc
     1561 agatgggttt tccctatctc gcctttgaaa aaatccagat agcctgatgc atcatcactc
     1621 ggctgaccga cagcctccac aaatgccgat ttgccaacgg tgttcacact gcggatataa
     1681 aagtaataat catggcccgg tttgatattg atactggcag ctatccagta cagcgccgtg
     1741 ccaagatagc gggctgtggt ttcaacctgc ctgatatcag caatccgctt ttccgagaac
     1801 cagaactcaa actgtaccgt cggatcataa accgcaagat gcggcgtggc ggttatctga
     1861 aaatagcccg gtgtcagctc aatccgagac ggcgctgccg gtgcggcaat ccggaacgat
     1921 accgatgccg gatcgccctg ctgtccccac gcatttaccg cccggactgt caacctgtag
     1981 ttccccggcg ccagttgcgt gaagcggtat gtggtttctg tcgtcctggc cgtgctgacc
     2041 agccgctcac tgccgtcatc cgctgccacg gtcaggcgaa gcaggaagct cacgcccttc
     2101 accaccttcg gcgtgtccca gcgcgccagc acctgatatt ccccgctgtc tgcggtgact
     2161 tcggcggtca ggtgctgcac cgctggcggc gtgacaccat tcaccgtgcc gctctggtcg
     2221 ccgtcaaagt gcgccccgtt atccacgatg gcttcttttt ccggcacgtg ctgcaccgcc
     2281 gtgatggcaa aggtgccgtc cgtgttttcc cggatggaga cacagcggaa caggcgacga
     2341 cgcagtgacg gcagggagag tccccataca ccgtatgtct ccacaccatc aggcagggtg
     2401 ctgacctgta tccggtccgg c
//
LOCUS       DAZFRJ010000353         2414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000353 DAZFRJ010000000
VERSION     DAZFRJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.355"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..561
                     /gene="eco"
                     /locus_tag="ACHKUN_004835"
     CDS             52..561
                     /gene="eco"
                     /locus_tag="ACHKUN_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO6662929.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(710..2356)
                     /gene="mqo"
                     /locus_tag="ACHKUN_004836"
     CDS             complement(710..2356)
                     /gene="mqo"
                     /locus_tag="ACHKUN_004836"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO6662930.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          564 a          666 c          606 g          578 t
ORIGIN      
        1 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa attgaagaag
       61 gaaagcaaaa aaatgaagac cattctacct gcagtattgt ttgccgcttt cgctaccact
      121 tccgcctggg cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct
      181 gaaaaaggga tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg
      241 aaagtagaac tgttaatcgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc
      301 gggaagctgg aaagcaaaac gctggaaggc tggggctatg attattatgt ctttgataaa
      361 gttagctcgc cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt
      421 gtcaccgcgt atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg
      481 gtgtatacgc cagacaatgt agatgtgaag taccgcgtct ggaaggcgga agagaaaatt
      541 gacaacgcgg tagttcgcta aaatgctgtg aagtgcggca ccccgtaggc cgaataaggc
      601 ggtcacgccg catccgacat ctaacgcccg agccggtgcc tgatgcgacg ctggcgcgtc
      661 ttatcagcat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
      721 tatccgccac ttctttttga acgggttgcg gtttcaactg cggttttggc gtactatctg
      781 ctgcctgcgg cttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
      841 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
      901 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agatccaaca
      961 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgcggca atggtccctt
     1021 gctggtcact gaccacttca gtacccagac gcagtacgcc acctttctcg gcatcacgct
     1081 tgataatctg cacgcgctgc cccgcttgcc acaaacgcca gtcctctttt tttgcctgcg
     1141 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
     1201 gatatttcac cagatcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
     1261 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
     1321 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacgcgggta tcgatatgcg
     1381 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aaccttcgcc agatggtgat
     1441 taaccacgtc cgggttttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
     1501 ctttcgcttc cggaatcccc gattcctgta atagcttcag cgccgcaccg cccgcgccga
     1561 taaagacaaa tttggcgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
     1621 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
     1681 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctcg ccatagttca
     1741 catcagtgcc aatttctgtg cgtgttgccg ctactttctg ttgtggatcg cgcccttcca
     1801 tcactaacgg tgcccactct ttgatctgcg cgtgatcttc agagtaacgc ataccgcgaa
     1861 acagcgagct ttgttgcaac gcggcgtaac gggcgcgcag gaaattaacg ttatcctcgc
     1921 cccagacaaa gctcatatgc ggaacggtat tgataaatga acgcggagta cgcagcacgc
     1981 cgcgctcaac ctggtgcgcc cagaactggc gggaaatctg aaatgcttcg ttaatggcga
     2041 ctgctttttc aatactgatg ctgccatcgg cgttttgcgg ggtgtagttc agttccatca
     2101 gtgcagaatg cccggttccg gcgttattcc agccgttcga actctcctgc gcgacaccct
     2161 ccaggcgctc caccatggtc atcgaccatt caggctccag ctcgcgtaaa taggtcccca
     2221 acgtggcgct cataatgccg ccgccaatca acagtacatc agtttcctgc tcctcggacg
     2281 ctttcgcttt tgccgccatc gaaacggcat taagccccac ggccatcgag aagagcatgg
     2341 cagtcacttt tttcatcttg ttaatgcctt acttttagtc gctttattgc aggtgagatt
     2401 tgcgcggcat caac
//
LOCUS       DAZFRJ010000354         2411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000354 DAZFRJ010000000
VERSION     DAZFRJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.356"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            258..401
                     /locus_tag="ACHKUN_004837"
     CDS             258..401
                     /locus_tag="ACHKUN_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO6662931.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            414..623
                     /gene="cspE"
                     /locus_tag="ACHKUN_004838"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             414..623
                     /gene="cspE"
                     /locus_tag="ACHKUN_004838"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6662932.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            789..1598
                     /gene="rlmA"
                     /locus_tag="ACHKUN_004839"
     CDS             789..1598
                     /gene="rlmA"
                     /locus_tag="ACHKUN_004839"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO6662933.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="ACHKUN_004840"
     CDS             complement(1595..2161)
                     /gene="mntP"
                     /locus_tag="ACHKUN_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO6662934.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          632 a          585 c          592 g          602 t
ORIGIN      
        1 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
       61 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
      121 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
      181 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
      241 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
      301 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
      361 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
      421 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
      481 ctgatggcag caaagatgtt ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
      541 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
      601 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
      661 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
      721 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
      781 gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
      841 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
      901 tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atacaagcac
      961 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
     1021 tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga tattggctgt ggtgaagggt
     1081 attacacaca cgcatttgcc gatgtgttgc ccgaaatcac cacgtttggc ctggatgtct
     1141 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt
     1201 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
     1261 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
     1321 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
     1381 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgat gagttgtgtt
     1441 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttgcct
     1501 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
     1561 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
     1621 tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt ttcccaataa
     1681 ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag gttgcacaac
     1741 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
     1801 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac
     1861 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
     1921 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
     1981 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
     2041 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
     2101 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
     2161 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
     2221 catgaacaac aagccgcgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
     2281 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
     2341 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
     2401 catattttga g
//
LOCUS       DAZFRJ010000355         2402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000355 DAZFRJ010000000
VERSION     DAZFRJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.357"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..241)
                     /gene="escO"
                     /locus_tag="ACHKUN_004841"
     CDS             complement(<1..241)
                     /gene="escO"
                     /locus_tag="ACHKUN_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO6662935.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQK"
     gene            complement(301..1641)
                     /gene="escN"
                     /locus_tag="ACHKUN_004842"
     CDS             complement(301..1641)
                     /gene="escN"
                     /locus_tag="ACHKUN_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO6662936.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1625..>2402)
                     /gene="escV"
                     /locus_tag="ACHKUN_004843"
     CDS             complement(1625..>2402)
                     /gene="escV"
                     /locus_tag="ACHKUN_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO6662937.1"
                     /translation="TVYFEAGHAEISFEFVQEKLSANSIVYKTNEANQQLAHVSGMDV
                     YAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLSKIVD
                     ILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVRGTLL
                     NVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPTGNGVILTALDI
                     RRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT          678 a          578 c          452 g          694 t
ORIGIN      
        1 gtttttgtga atcgagcaat gaacgctttt ctttagaaag tgaggatttt ttctcatata
       61 atcgctgctt ttgctcatca aatgcattgt ttttaatctg gtatttatac aacgacactt
      121 tatccagatt caccagcgat gcacaatacg cctgcttaga ggcaatcgac tctttaatgg
      181 cttgctcgca gtcatcgagt ttaccatcca cctcttttat ctgagagtta atcttagcca
      241 ttgattctct caatcgattc gctctgcttt tacgaataga taaaattctg tccaacatat
      301 tcaggcaacc actttgaata ggctttcaat cgttttttcg taactactga tatctttggt
      361 gctttgttgt ataaagctct gaattgcttt cctgtttttt attgccttat ccgcttcagg
      421 atcttgcccc atcgtgtact caccaatacg aataagcagt tcaatattct tataggttgc
      481 aataagcttt ttgcattcag ccgctgcacg taaatgttcc gatttaacaa cgttatgcat
      541 aacacgactg gcgcttaaac caatatcaat tgcagggaaa tggttttcct ctgcaagttc
      601 tcgggtaagc acaatatgtc catcaagaat agagcgaact tcatcaccga tgggatcatt
      661 cacattatcg ctttctaaca gcaccgtata aatcgcagtt attgaacctt ttggcgcagg
      721 tcccgcacgc tctaataatt tgggtaacga ggaaaaaaca ctcggaggga atccccctct
      781 tacatcaggc tctcccgatg caagcccaac atcacgcgcg gctcgggcat aacgagttac
      841 ggaatccatc attaataata catttttgcc ctgatcccgg aagaactctg caatggttgt
      901 cgccgtaaat gcggccttca ttctttccag cgcgggacgg tctgacgttg tgacgaccag
      961 tacacattta gaaagcgtgg attgaggtaa aagcgcgagg aattcattta cttcgcgacc
     1021 acgctcgccg ataagggcaa ggacaataat atccgcggat gcgccattac aaatcattcc
     1081 caaaagcgta cttttaccaa cgccggaccc cgcgaaaata ccgatgcgtt gcccaatacc
     1141 gcaagtaagt aaaccatcta ttgccctgac gccaagcgta aatggctgat caataacctg
     1201 ccttaataaa ggatcggggg gttcagcata caaactgcgt tcaaacggaa gataaggtgc
     1261 agtaatattg ctccccattg gtctgcctat accatcaacc agtcgcccaa ggagttcgtc
     1321 cccaacacga atcttgaact cttccccctg ataacttagc cactggccac aatacatgcc
     1381 ggaaatatgc tcaaaaggca gcaggaaaac ctcatcttca tcaatagcga tcacctcagc
     1441 taaacgttga ctgggttcta tcttgtaaaa ggcaccaata cgcgcttttg gtaaacgcgc
     1501 cttgataatc gtcccgccga tattggtaat cttgccttca tatctggtag acgaatttaa
     1561 tgataatgtc ggcacagtgc tattgagcac tttctggata cgtgggtatc tttccaatac
     1621 agaatcatgc tctaaaatca tttaccgttc ctaatacttt aagttcgata ttattcccaa
     1681 cttcctgaaa agagagcacg gggactgacg ggaacgaacc ttcaatcatt ttcttcacat
     1741 agcgcctgat atctaaagcg gtcagaatga cgccattccc cgttggattc acaatatttt
     1801 ttaagaagcc aataatctgc tcacttcgct ccggtgagat attcaaatac gaacctgatg
     1861 acgtttgtct gatcgactct cgtaactcat tttcaatatc agagccaata agccagacat
     1921 tcagaagtgt accgcgaacg ctatagcgac ctaaaatatg ccgacggagg gcgatacgaa
     1981 cgtattcgca caagataacc acatcttttt cttttgtgga ccaaaaaata agcgtttcaa
     2041 aaatagttcg tagatctcta attgagatat tttcctctac gagacgctga agaatatcca
     2101 caatcttgct cagaccaagc tgacgctgaa gctctttcac aagttcgtta tatttcctct
     2161 ccatgatgtc catcagataa cgcgtttctt gtacgccgat gaactctttg gcattagaca
     2221 atacaagttt ctttagcaaa aacgttatct tgtcatttgt ttttgcataa acatccatac
     2281 ctgagacgtg agctagttgt tggttagcct cattcgtttt atacacgata gagttagccg
     2341 aaagcttttc ctggacaaat tcaaatgata tctctgcatg cccagcctca aaataaacag
     2401 ta
//
LOCUS       DAZFRJ010000356         2381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000356 DAZFRJ010000000
VERSION     DAZFRJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.358"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(410..541)
                     /locus_tag="ACHKUN_004844"
                     /pseudo
     CDS             complement(410..541)
                     /locus_tag="ACHKUN_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            823..2181
                     /locus_tag="ACHKUN_004845"
     CDS             823..2181
                     /locus_tag="ACHKUN_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO6662938.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          626 a          570 c          606 g          579 t
ORIGIN      
        1 ttctttttgt cagatgttaa tcacaccaag aaagatgttg ttcagagcgc acttaatatt
       61 atcattgaca ttacatcagg tgatatttga gattttacat aaaagataat ctctcaagga
      121 gtcttatctg aaaacgaaat atatagtccc tctcgttttg taatatcgat tggctttgtt
      181 gaatcggtta cgtaccccgg agccggtcat attttcaggt aatacacacg ctttcagaca
      241 tttcggcttt cgtcattttg gtatcatggg catagcttct gctacctgac catcataatc
      301 acgcagcttc agtgaagcag tgaccacctg tttaacccgg tgtatcgtta tgatcttatc
      361 cagcaattga accgccccgg gtttcctgga gagtgtttta tctgtgaact caggctgcca
      421 gatcatcgtt tccgatggaa gcataataag ctttttctgc ttctgccggt ggaatatggc
      481 cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt gtggtcagct
      541 catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa tatagggcgt
      601 ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt gatcacgaag
      661 aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt gttttatatt
      721 tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac tcgtttcttc
      781 tttatggtgt agtgtgatct tcactttatg gatgctaaca atatgaaaag aaaaattatt
      841 cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc tacggctgag
      901 cgctatgtcg tcagttttcc agaaggcacg cacgtgaact atgccggcgc atttgccagt
      961 gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac cggtaaacag
     1021 ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga ttcaccgaaa
     1081 gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct gctgatgacg
     1141 attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca tgacgataaa
     1201 ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa tgaagtggcc
     1261 ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac ggaagggatc
     1321 actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt cctgattaac
     1381 atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga aggggaaaaa
     1441 gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg cggctttgaa
     1501 ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac gctggatatc
     1561 gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga tccggcaagc
     1621 ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa aaacagcgac
     1681 gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat cgaacaggga
     1741 agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa taaggcgagc
     1801 gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga gaaaaccctg
     1861 tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg ctccttgggc
     1921 tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct ggcggtggcg
     1981 aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg caagacattc
     2041 aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca ggttgaaacg
     2101 accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg gattgtgacg
     2161 ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct tagtttttca
     2221 aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa agcgtcacca
     2281 cgtgaaaaca tgcctgcacg gtttacacgt catctcatgg tggcagccgg ttaactttcc
     2341 tgctacacca gtggaaaaag gccataagca gtccgaacaa a
//
LOCUS       DAZFRJ010000357         2375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000357 DAZFRJ010000000
VERSION     DAZFRJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.359"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1827
                     /locus_tag="ACHKUN_004846"
     CDS             <1..1827
                     /locus_tag="ACHKUN_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6662939.1"
                     /translation="DGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPE
                     KEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFS
                     LRLTSGSGEDSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRIN
                     APAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGS
                     QWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLELFREKIGKTHLA
                     EALWAEIDNSQLKDEMAEMQTTITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDD
                     LAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLF
                     VAQGNQLFMNDVFLKRLFAVSITSSGNPPAFSLTPDGRLTAKNADISGSVNANSGTLN
                     NVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMI
                     PPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDVQGVFSSVIDMPAG
                     QGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            1894..>2375
                     /locus_tag="ACHKUN_004847"
     CDS             1894..>2375
                     /locus_tag="ACHKUN_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6662940.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     G"
BASE COUNT          592 a          598 c          697 g          488 t
ORIGIN      
        1 gatggcgtgg agacatacgg tgtatgggga ctctccctgc cgtcactgcg tcgtcgcctg
       61 ttccgctgtg tctccatccg ggaaaacacg gacggcacct ttgccatcac ggcggtgcag
      121 cacgtaccgg aaaaagaagc catcgtggat aacggggcca gctttgagcc gcagtcaggc
      181 accctgaaca gcgttattcc accggcagtg cagcacctga cggtggaggt gagcgcggct
      241 gacggtcagt atctggcaca ggcgaaatgg gacacgccgc gggtggtgaa gggtgtgcgc
      301 ttcagtctgc gcctgaccag cggaagcgga gaagacagcc gtctggtgac caccgccatc
      361 actgcggata cagagcaccg tttcagtggt ctgccgcccg gggaatacac cctgacagtc
      421 agggcaatta acagttatgg ccagcagggc gaaccggcca ccaccacctt ccggattaac
      481 gcgccagcaa aacccgccac cattgaactg acgccggggt attttcagat aacggcggtc
      541 ccgcgtcttg cggtgtatga cccgacggta cagtttgagt tctggttttc ggaggcaaaa
      601 atcgcagaca catctcaggt ggaaacctct gcccgttatc tggggaccgg cagtcagtgg
      661 agtgtatccg gcccgcacat taagcccggg aaggatttct ggttttacgt gcgcagcgtc
      721 aacctggtgg ggaaatctgc gtttgtggaa gtcagcgggc agcccagcaa tgatggtgaa
      781 gggtatctgg aacttttccg ggaaaagata ggaaaaacgc atctggcaga ggcgctgtgg
      841 gcagagattg acaacagtca gctgaaggac gagatggcgg aaatgcagac caccatcaca
      901 gaaacccgca atgaaatcac acagacggtc agtaaaacgc tggaagacca gagcgccacc
      961 attcagcaga tacagcgcgt gcagaaggac acaaatgatg acctggctgc gctgtacatg
     1021 ctgaaggttc aaaaaacgaa agacggcatt ccctatgtgg ccgggattgg tgcagggatt
     1081 gaggatactg atggccagcc actgagcaac atactgctgc tggctgaccg tatcgcgatg
     1141 ataaatccgg agagcggcaa cagcacgccg ttatttgtgg cgcaggggaa tcagctgttc
     1201 atgaacgacg tgttcctgaa acgactgttt gcggtgagca tcacgtcatc cggcaatcct
     1261 ccggcatttt ccctgacgcc ggacgggcga ctgacggcga aaaatgcgga tatcagtggc
     1321 agtgtgaatg cgaactcagg gacgctcaac aacgtcacga ttaatgagaa ctgtcagatt
     1381 aaggggaaac tgtcagccaa ccagattgaa ggcgatattg tcaaaacggt cagcaagtct
     1441 ttcccccgca cgagcactta tgccagtggc accatcacgg taagaatcag tgatgatcag
     1501 aagtttgacc ggcaggtcat gataccgcca gtgttattcc gcggtggtaa gcatgagaat
     1561 ttcaacagta ataaccaaca gtcatactgg tattcaacct gccggttaag agtgacccgc
     1621 aatggtcagg agatttttaa tcagtccacg acggatgttc agggcgtatt ttcctcagtt
     1681 atagatatgc ctgccggaca ggggacgctg acactgacat tcaccgtatc ttcatcagga
     1741 gcgaataact ggacaccaac aaccagtatc agcgatctgc tggttgtggt gatgaaaaaa
     1801 tccacagcag gtatcagtat cagctgaatt ttataaccca gaacgggcgt cagaaatgac
     1861 gcctttttta ttgcagaaaa gcgagaggta attatgcgta aactttatgc cgccattttg
     1921 tccgcagcca tttgtctggc cgtatccggt gcgcctgcat gggcgtctga gcagcaggcc
     1981 acgctgagcg cggggtatct tcatgcccgg acgaacgctc ccggtagcga taatcttaac
     2041 gggattaacg tgaaataccg ttatgagttt acggatacgc tggggatggt gacgtcattc
     2101 agctatgcag gagacaggaa tcgccagctt acccgttaca gcgatacccg ctggcatgaa
     2161 gattccgttc gtaaccgctg gttcagcgta atggcggggc cgtctgtgcg cgtgaatgaa
     2221 tggttcagcg cgtatgcgat ggcgggtgtg gcttacagcc gtgtgtcgac tttctccggg
     2281 gattatctcc gcgtaactga caacaagggg aaaacgcacg acgtgctgac cggaagtgat
     2341 gacggtcgcc acagcaacac gtctctggcg tgggg
//
LOCUS       DAZFRJ010000358         2362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000358 DAZFRJ010000000
VERSION     DAZFRJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.360"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..507
                     /locus_tag="ACHKUN_004848"
     CDS             <1..507
                     /locus_tag="ACHKUN_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662941.1"
                     /translation="SSKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLI
                     PGYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQ
                     RIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLES
                     GGNWMDNL"
     gene            582..1571
                     /locus_tag="ACHKUN_004849"
     CDS             582..1571
                     /locus_tag="ACHKUN_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662942.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          586 a          589 c          568 g          619 t
ORIGIN      
        1 tctagcaaag ctagtgcgtt attcaataac caagactgga aactgtcagt caccgacatc
       61 aatctctacg agaatactgt gagtctggac ggtcagttct atccgctgtc cctcgctatc
      121 aagaccctga ttccgggcta cctgtcaggc ctgccgtcta ccagccgcga gtctatggag
      181 ttattggaag ccctggctga agctggcgtg accattggta acttcttcag taatgacctg
      241 atgaccgcct atggtcgccg ccagcagaac aagcgagctg aggctgagcg tatcgctaaa
      301 gagcagcgca ttcaggccga gcgcatgcgt gaagagaaca tgactgatgc agagtggcag
      361 aaagagctac agcgccgtga acaggtcaaa gctgaacgcc ggacctacgg tgagaacctt
      421 cgtagtgcta cccattccgc tggtcgctcc cgtgccgcta tcgtcgctga ccttgagtca
      481 ggcggtaact ggatggacaa cctgtaagac agacgcacac aagtggtcct gacctgatgt
      541 cgggacccac gtatctaaac taatttagga gattgattat tatgactatg actaaagaca
      601 gctatcagtt cggcattgag ccagtacgta tcaccgacac agacaacatt caggttaacg
      661 aaggtctgcc aaccaatgct gacccacagg tctatgctct ggcactggct aaagctgtca
      721 agaccatgct gaatggtgtg ctgaagtccg ctcaggataa cattccattc ccgattgaga
      781 ttctgccaac tcgcaatagc ttaccgaccc caatcattgc tcacactctg gctgaccgct
      841 ctgtggtcgt tcctgtccgt ggtggtaagc gacctgaagt cgtgaccgct ccgtctggta
      901 ctgaaatcac cgttgagccg attgagcaag ctatcctcgt gtcccaccag accaagctat
      961 gggacgctaa gtcttccact ggtttcactc aggggacgct ccagcaggac gctctgaaca
     1021 tctgcgataa cgtggtccgt accatcaata gtcgcatggt cgatgtactg gaatcctcca
     1081 agctgttgaa gactgtagag cttccggtcc tgactggtag cctgacagct aaggcagaca
     1141 ctatcatgga cgcgctgtac gagaacaccg agccttcttt cggttctgag gttgctgatt
     1201 acggcatcat cgcccatgag tcacacctca aggcactgtc acgtcttgct gctaagcagg
     1261 gctttggtgg tgaagacgct atcgtcgata tgttggggac tgacatcgcc tattacaacg
     1321 gtgaagaccg tggcgtcttc atgatggcta agcgttttac ggctctgagc ttcggttgct
     1381 tccgtcacga tggggaccac attactgtgg tcctttcacg tgatggtgac tcccagtctc
     1441 acgaccttga gattctcggc aaggtgtttg ttgtagctga agctgctacg accatcaaga
     1501 tggggactgg ctcggctact gctgtcctgc ctgtagtcaa acgcctgagc ttcactaaag
     1561 aagctaacta acaataacga ctattggtca gacttccgaa ctggatgtct ggcctttatt
     1621 ttgtactagt aatctagttc gtgaatacag aagggaggag gaccaaatgg acctacacaa
     1681 agtctatctc gtggtcttcc tgctaaagct gggactggtg gtctggctat gggctattag
     1741 acgatgagtt ttgactgaaa aatgtgagac ctcatctaat ataaagagcg agccaaaagt
     1801 ccccatagcc cgaccctgag tcagaccaaa agggccgggt agtcagaccg tgagttctat
     1861 tcctttagag ggaatattac gtgcttcaag tcttgaccgt ctatcgctaa atgtgtgact
     1921 atcactacaa ccaactggat tcatcagtct ttaagaaaca agaggttagc tggttgtctt
     1981 cgatttgtcg taggctccct tcgcccgctc caatttatct tcacctgatc aatctgccat
     2041 ttttgctgta tctgattttc tatattatct taattcaatc gcttcagact tttcgcaatc
     2101 gccgtaatac ttccccattt tgttgtaaat aaaaagcgtt tacgcaatgc aatccttttg
     2161 taacaaccca ggtaaaacac cttattttca agttgttaaa taagtggtta tctacgcttt
     2221 taacaaacga ttgatggaat attttatgaa agggaaatct gcattaactc ttctgctcgc
     2281 tggtatattt tcgtgcggca catgccaggc gacaggggca gaagtgacga gtgaatctgt
     2341 atttaacatt cttaattcca cg
//
LOCUS       DAZFRJ010000359         2322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000359 DAZFRJ010000000
VERSION     DAZFRJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.361"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            479..706
                     /gene="ykgL"
                     /locus_tag="ACHKUN_004850"
     CDS             479..706
                     /gene="ykgL"
                     /locus_tag="ACHKUN_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO6662943.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(741..881)
                     /gene="ykgO"
                     /locus_tag="ACHKUN_004851"
     CDS             complement(741..881)
                     /gene="ykgO"
                     /locus_tag="ACHKUN_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO6662944.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(881..1144)
                     /gene="ykgM"
                     /locus_tag="ACHKUN_004852"
     CDS             complement(881..1144)
                     /gene="ykgM"
                     /locus_tag="ACHKUN_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO6662945.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1508..1609)
                     /gene="ykgR"
                     /locus_tag="ACHKUN_004853"
     CDS             complement(1508..1609)
                     /gene="ykgR"
                     /locus_tag="ACHKUN_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO6662946.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2083..2172)
                     /locus_tag="ACHKUN_004854"
                     /pseudo
     CDS             complement(2083..2172)
                     /locus_tag="ACHKUN_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          677 a          437 c          518 g          690 t
ORIGIN      
        1 tatattggtc aggaactttg ccctgaagtg ttaggaatag tcttaatgcg ctgtgtgatg
       61 gactagtaaa aaaacaagtt ttaagggtaa gggatagaat ataaaactat aatgagctgt
      121 cattgaagat gatttgccaa tcagtaagaa attttaatgg attaacatgc cgcacaaaat
      181 ggttataggt gttcattaca ataagtgatg gaaaattgaa cgggcgtatg tacgtcatct
      241 gttcggtaag cccccaagag ccagattaag tgaagttaaa cgaaggctgc cagctgtcac
      301 aggaacgatg gcctctttgt cagggcactg agtgcagtta tctgtgcgcg ttactctaac
      361 tggcactcga acgtcggcta cgggttaccg gtggatcaga ctgttcctgt ggcgtaaacg
      421 tttttatgtt agtaccaaca gatttaccac atttatcatc ttaacagttt gagtgaaaat
      481 gcaggctgcg ccgcaaaggg aggagactga gtggcgtgtt cagtctaaaa gagggttgat
      541 gccagcatac aggggcgaag ctggtcaaca agtcaacatt aagataatgg aatattcgga
      601 gagaaacgtc aggcagttgg cctcaaatga acaggaagaa tatatcccca ggaaaattaa
      661 tgttggtgtt ataaacacac caacattaat ccgatctgat tattaatcag acaaatatcg
      721 ttaattcgtc gaattttgaa tcaacgtttt ttcttacgac cctgaacggc cttaaaacgt
      781 ggattagatt tacaaatcac atacaaccgt ccttttcgct tcacaatctg acagtctgga
      841 tggcgttctt ttgcggtacg cagagagtta agaactttca tcacgccccc tttttcgtgc
      901 taacaaaacg accaaaacgt tgggtgaatc gtgcaacatt tccttctgat gtcactgttc
      961 tcagcttccc tgtatagaac gggtgcgatt tagaagagac atcaattgtc acgtacggat
     1021 acgttacgcc atccagctca atctcacggt ctgttttgat agtcgagccg attttaaagt
     1081 actcatcaac actggtgtcg tggaacacca cagtacgata ctcaggatgg atattgggct
     1141 tcatcatttt tacctgttat gttataacat aaccgtaaag atacatgctc atccggatca
     1201 tttcaacacc caatccctgt gcccatttgt ggtatgtgga atttccccaa cggggaagtc
     1261 tggcattgtt agcagccgtt ggcgacgctc ccataaacgc ctggatgagt gattgatgat
     1321 ggttttgctg agagtatgga tttttcgaga atggaggtta tctccacttt actggccgat
     1381 gatttatcgc agtatgattg attacctgac agcgtgtatt cctctcccac ggatataata
     1441 attacttaca aaaagggaga ggatgcatat tttaaatatc actgaagtga acagtttatt
     1501 gccgttatta atagaaatgg agaaataaat aggcgtattc tacaattgcg acaaaaacaa
     1561 cgatattaat cagtttatga ctgatttgtt gtactttatt ctctttcatt ggtacttcct
     1621 cgctttaaaa aagagtgcac ttcgtaagtg cccttatata aataacgagt ttggtcaacc
     1681 aattttttga catgtatcac aaatttgaat agatgtatta catcagctat cttttattgc
     1741 accaacgtca ttgatatatg tcgactgaag tcagttccgg gaatgagtct gatctcaaga
     1801 ctggcccagt ccgggcgttg attggcgctg aggagcatac ctcatttcat cataatgtcg
     1861 tatctcctgg ggtgttatac aagatatcgt tgttggtaac ctgggaaagg aattgagttc
     1921 tattaaaccg tcaactatgc cggatacata ctggattaca ctgcaggcac gccttatgag
     1981 agaacgtgcc gcagtgacgg gttaattatc tgaaagaatt tgtgaggctg tatcggttac
     2041 tcattgattt gatagtttta ctctcgggag aataatagat atttaatcca ttaacggaaa
     2101 ccagccagtt cctttcgatg cctgaatttg atcccatagt ttatccggaa tggttaattc
     2161 atgaacaagt tgtgttatcg ttcatgagaa gcataacgta aagggaaaag ctcgattaga
     2221 cggcagaatt tgtcaggggt tatgaacgaa attcataaat ctgtttgagt gttgcgatgg
     2281 gtagtgcaag ttcgatatct ccgcaattta cagtccgatg aa
//
LOCUS       DAZFRJ010000360         2321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000360 DAZFRJ010000000
VERSION     DAZFRJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.362"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(541..726)
                     /locus_tag="ACHKUN_004855"
     CDS             complement(541..726)
                     /locus_tag="ACHKUN_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6662947.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
     gene            complement(954..1103)
                     /locus_tag="ACHKUN_004856"
     CDS             complement(954..1103)
                     /locus_tag="ACHKUN_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662948.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            complement(1103..1672)
                     /locus_tag="ACHKUN_004857"
     CDS             complement(1103..1672)
                     /locus_tag="ACHKUN_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO6662949.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            complement(1947..>2321)
                     /locus_tag="ACHKUN_004858"
     CDS             complement(1947..>2321)
                     /locus_tag="ACHKUN_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6662950.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
BASE COUNT          626 a          615 c          454 g          626 t
ORIGIN      
        1 gtgatcatgt agcataagta acctccgttt atctacagac tgccatcctg aatttaccgt
       61 ccttaataag acaacaataa aaaactaatt atataaaaac aataaaaata atatacaaaa
      121 aaactaaaca acaaagaaaa gcaaatcata tctggcattt taattgaaag agcaattacc
      181 gaacaaaaga cgctgactat atactcaaaa ccaaacaaca attctgccaa tcaggtatca
      241 tggcaacaca cggaattacc atgtttttgc cttctctgcc catacaatac gggcatatac
      301 ttcatactct attgtaatat ttctatccat gcgccccact ccatttacct gtaaataata
      361 ttcaaaatat ttatcacaga aatcgttttt ggccatgaac tgagcacact ataaagtccg
      421 gaactgactc tttgttaaat taccttaacg ttaccagtaa caccttcgta acaaaacatc
      481 acggtataca ctgggtacgg atatattcct gtgctccttc cagttgcttc tgcattgcca
      541 tcagccgttc tctgaggatg aaataatccc gttcagcggc ttctgccagt cggggaccgg
      601 ttgcattatc cacgccggag gtgatggggg ctttacgcaa ggagcctgga cagttggcgt
      661 tgatgcgcag gcgcttacga ccagcggcaa catcagcacg cagagtttca ttttcagctc
      721 tcgcatcggc taattccctc gagtatctgg catcaagtgc agcaacatca cgctggcgct
      781 gctgcatatc agtaatggtt gcatttgcca gctccagctc actgactttt ttatcgcgct
      841 gctctttgta ggttatggcg ttatcgcggt aatgattcag ccccagacta agtgcaccac
      901 aggctaccag caggacaata atcaccacac acagaacacg gttcatctct ctttcacccc
      961 accagtcccg ataacgtcag gactcgccaa gcggtggaaa agaaaatggc aaccagaatg
     1021 actaaaaatg aaatgccgac gattacacag aggatcttcg ccagcgttat gagtttatcc
     1081 gatatcatta gccaccactc catcaatccg cctttgttat tttccctttg tctgtatcag
     1141 ccaggacaaa atcaatcagc agattcgctt cgtttatcaa tgtgcggatt tttgatacat
     1201 gcgcggattt aacctgtttc cactcattca gcccggtagc aaacacactg gcaatgtttt
     1261 tatcccgttt catgtcagcg caagcctgat tgagttcttc catcacactc attcgacggg
     1321 gattaacgac aaaacccttc gtccagtatt catagagaac atcgtcacac tcttcctgat
     1381 actggattac cttgccgcgg atttcgggtt ttactttgtt gggattgatg gtttgtaacc
     1441 agcctgcaag ttttcgaagc ggcagggaca ccatattgcg tcgtttccca tcctcagcaa
     1501 ccataacgat ttccgttata gttgacgcaa aacgctgtct taacttagcc aactgtgatt
     1561 gccaggccag ccccatcccc gcaacgacag gtttcatggg aacgtatggt tcgccgttat
     1621 ggttaaccac ataaagagaa tcgccgtgaa acggcacggt catcatattc atcggttatt
     1681 tccttttagt gatgaaccct gcgcacagga ataaccagcc caaagagggt taaccagacc
     1741 actgccggtt atccaccagg gctcatcctg aaaggttctt tggtttattt acgcttgtgc
     1801 gaagcgcaga aatgacaaag gcaccattac ggtgcctctt catgaaacaa tcttgttgac
     1861 tttattcact tacattttgc cagttcgcag gatttcgtgt tgtctacccg cgttggccaa
     1921 cgtcattttt cagcaaaata ttctgcttac ctgtcgatac cccagcatgc cagcgcactc
     1981 tcctggtcac gtcttgatac ctgaccgtaa cagttgtttg aacggatacg gcagtctctg
     2041 ccaccgtcct taatccacca gcgaatcgcc tcacacgctc ccctgcgatc gcctgcatta
     2101 agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata ggggcaaaat
     2161 gacgcgatac ctgctttttg tggctcggtc agtggaactt ttatattacg ctccacccat
     2221 gccagcgcct tatcccgttc gatagcgtta acccggtcgc atttttcctt cgacagcttc
     2281 atgccaggaa tcacaggctt accatccacc atgatggcgc c
//
LOCUS       DAZFRJ010000361         2285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000361 DAZFRJ010000000
VERSION     DAZFRJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.363"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..678
                     /locus_tag="ACHKUN_004859"
     CDS             <1..678
                     /locus_tag="ACHKUN_004859"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6662951.1"
                     /translation="PPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVG
                     RAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRG
                     LIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLN
                     VDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAIMDF
                     MTARGYH"
     gene            complement(727..2226)
                     /gene="pitB"
                     /locus_tag="ACHKUN_004860"
     CDS             complement(727..2226)
                     /gene="pitB"
                     /locus_tag="ACHKUN_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO6662952.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          610 a          597 c          559 g          519 t
ORIGIN      
        1 cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
       61 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaagcc
      121 ggaaatgtcg gtcgtgcagt gacaaaatta ccgttgatgg gaaaatcctt acataaaaca
      181 attgaacgaa atcaggtgaa aacagcaaaa aaattgccag gaccagttcc ggcgctggta
      241 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
      301 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
      361 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
      421 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
      481 gttgaccttg aagttgcctg cgcgcgtaag cctgaccaca aaagggagtc gctggcaaga
      541 aagatagcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
      601 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattatgga ttttatgacc
      661 gcgcgtggtt atcactagcc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
      721 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaatcgccgc
      781 cggtaaagta aatacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
      841 gccgtccacc accatcgtcc ctgcaactgc agacgagagg acgtgtgttg tggagacggg
      901 catcccaata taactggcaa gaccgataga cactgccgcc gtcatttgtg ccgccatgcc
      961 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
     1021 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
     1081 agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc
     1141 tttactgacg cctggcagtt tcgccagctt cgcggaggta tctgagatgc acagcataat
     1201 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
     1261 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
     1321 tgttacttgc gcgccatcag tcgatgccgc aggcaatgga ggttccatcg caatcaactt
     1381 ctgcggcagt tcaggatgct gttgcagata gtgttcgaag ttggagacgg catcgcgagt
     1441 acgggtaatt tcatagccgg acgcgttcat attgacgacg aagccagcag gggcaatccc
     1501 caccagtacc agcatcacca ggccgatccc tttctgcccg tcgttcgcgc cgtgcgaaaa
     1561 cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
     1621 gccttttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
     1681 gctccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg
     1741 ggaaacaatc agcgaggaga aaattttggt cacttcacgc aggtttaacg catccatcac
     1801 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcgc caatcaaggt
     1861 gtgcgaactg gaggccggta aaccgaagaa ccacgtcccc aggttccata taatcgccgc
     1921 cagcagcatg gaaaagacca tcgccagacc gtgggttgac cccatattca gcaacaaatc
     1981 ggttggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
     2041 aaagttaaaa aatgccgcca tcaccacagc aagttgtggt tgcatggcac gagtataaat
     2101 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
     2161 aaatgccaga gcaagcaata acaaaagccc tgtgtatata tcaaggccaa caaataaatt
     2221 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
     2281 acggc
//
LOCUS       DAZFRJ010000362         2284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000362 DAZFRJ010000000
VERSION     DAZFRJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.364"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1822)
                     /locus_tag="ACHKUN_004861"
     CDS             complement(<1..1822)
                     /locus_tag="ACHKUN_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6662953.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNELNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKVQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQ"
     gene            complement(1870..2079)
                     /locus_tag="ACHKUN_004862"
     CDS             complement(1870..2079)
                     /locus_tag="ACHKUN_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6662954.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(2175..>2284)
                     /locus_tag="ACHKUN_004863"
     CDS             complement(2175..>2284)
                     /locus_tag="ACHKUN_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6662955.1"
                     /translation="PDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          446 a          768 c          559 g          511 t
ORIGIN      
        1 gctgctgaag cagttcattc cccagcccca gcagacggtt gaattccgta tgctgaccgt
       61 tcataaccag catggactgg tacaccttat tctgctctgc cgcctgctga cgaattaacg
      121 ccacacgacg gtcttccagc ccggcaagca catcctgaat ggactgcact ttttcctgca
      181 tctgtgccag acgggactgc tcaacggcaa gctgctctgt tgcctgagaa agcccttccg
      241 tcacggtctt caccgatgtc agatggttta tcatgaatcc gtcaccggtt gtccagccag
      301 ggttagccag aacatactga tatccagcga ttttttcctg cagggatttc acccggctgg
      361 cctgttcatc aatcagccgg ttctgctctg ccagcgccgc ccgtgttcgt ccttcattat
      421 ctgaggcttc aggcaaagac attgacggcg ttttatgcgc gatttcatct atcgtcagtg
      481 catactggcg cgctgactcc ctggcctgct cctgattctg gtacagcgta taccatgctg
      541 ctgcccccag catcaccagt ccgggtacgc caccaaccag tcccaacgca ccagtcatca
      601 gacgtgagcc caccgccgtt gtactgttca gctcattctg ggctgcggtt ctggcagcaa
      661 tatttctgtt caggcgttcc tgtgtggccg ccagacgggc ttctgcagca atctgcatct
      721 ccgtcccgcg ggctgccgcc acagcctgct gtgcacggta cacggctgcc cttgcccgcg
      781 ccgtggcaat ctgcgtcccc ctgagctgtg cttccgccag tgccacttca ttacgtgctg
      841 ccgtcacaag tcctgccgtg gcagacaccg ctccggaggc catattgcca aagtaccggg
      901 caaccccgac ggcaaccagc gcccccgcgg ctgttgccac attatcaata ttaccggcaa
      961 caccgttcag cacgccggag agcgtttttg tcaccccgct ggcttcattc gcgccaccca
     1021 cccaggccat aaaggcgttt tccacctttg tgatcccgtc agagaccgtt tccggcatgg
     1081 cggcatattc atcacgcaat acccccagct ggctgattaa cgcaggaacg actttatccg
     1141 ccgtcagttt gccgtcgtcc gccatcgcct taaggtcttt acgggccacg cccatacccg
     1201 cagccagtgc acgtacgatc cggtctccgc tttcattgac cgaattaaat tcctcaccgc
     1261 gtaacacacc ctgtgccagc gcctggctga actgggtgat caccgagccc gcctctgccg
     1321 tactggcacc ggagattttc agccccgtgg aaatggcctc cgtcaccttc agcacatcat
     1381 cagcactgta accatattca cgcattgagg ctgccgagcg ggcaaacagg gccgcattat
     1441 ctgaaaatgc cgtgcccgtc cgctgactga tatccatcag cactttctgt gatgacgaaa
     1501 attcatcgga tgactgcgac gcctgtttca gtcgggcatt cacggaactc cattcatcgg
     1561 ccagagaaat caggtgtccg gtggcaaagg cacctgcaaa tgcccccgcc gttccggcag
     1621 ctgaagcgcg gatttccgtc aactggctgt tcagctcagc cagggcgcgt cgctgctccc
     1681 gggcgactgc ggcagcctga cgcccgccat tctgcagggt ccggtaatat tcactgccca
     1741 tgcgggaagc ccgctggatc tccgactgga atgactgcga atttgccgaa attttgataa
     1801 tcagttcacg taacgtcgcc attcaccttt ctccgggcgt aaaaaaaccg cctcagcggt
     1861 tctcatcatt catgactgtg ctgcgaagct cagcgcgtct tccagcgccg caaacggatc
     1921 cacctccggc ttatcctcat cctcgcccca gcagagcatg gcgtccttca gtgcaacatt
     1981 catcccctgt gccccaaaaa ccgctttcac gatctgtgca ttacggatat ccccgcgctc
     2041 atcacccagc ggggataccc tgtcgaactc catccacatc atcgcctcgc tcacactcag
     2101 gctgtgccgc agttcggata aggtgcgccc cagacggagc gcaagtcgca tcagaaagcg
     2161 aatttccggg cgggctactt ttttctggcc gactctgcat cagcgatcag ttccagtgcc
     2221 tgacgcagca accgggcatg taccggacca tagacggcca gcacctgctc acggtcgtcc
     2281 ggag
//
LOCUS       DAZFRJ010000363         2270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000363 DAZFRJ010000000
VERSION     DAZFRJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.366"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..346
                     /locus_tag="ACHKUN_004864"
     CDS             74..346
                     /locus_tag="ACHKUN_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6662956.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            423..638
                     /locus_tag="ACHKUN_004865"
     CDS             423..638
                     /locus_tag="ACHKUN_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6662957.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            643..987
                     /locus_tag="ACHKUN_004866"
     CDS             643..987
                     /locus_tag="ACHKUN_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO6662958.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1038..1571
                     /locus_tag="ACHKUN_004867"
     CDS             1038..1571
                     /locus_tag="ACHKUN_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6662959.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1731..1868
                     /locus_tag="ACHKUN_004868"
     CDS             1731..1868
                     /locus_tag="ACHKUN_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662960.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            1870..2208
                     /locus_tag="ACHKUN_004869"
                     /pseudo
     CDS             1870..2208
                     /locus_tag="ACHKUN_004869"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2180..>2270
                     /locus_tag="ACHKUN_004870"
     CDS             2180..>2270
                     /locus_tag="ACHKUN_004870"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6662961.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          585 a          513 c          656 g          516 t
ORIGIN      
        1 gccggaagca aacccgaaga gccgctcatt tagcggcaac tttcttaatc acatctttcg
       61 acgagaaaat cccatgtcag aaattacatc cctggtcact gctgaagcag tgaaggaagt
      121 cctgcgctct gaagaagtcc ggagcgcact gaaacaaaaa cttcgccata acctggaagc
      181 gcgtcttgat gcagaggttg atgccattct ggatgagctg cttggtgtac aggcagagcc
      241 accgactgaa gcgggagata ccaccgcaga gagcggtgaa gttcagcctg aatcaccggt
      301 cgccgatgcg actgaacctc aacccgaatc ggtcatgatg ctgtaacggg gagtcagggc
      361 catcagtagt taactggtgg cttttttatt gttgtcagct tccggataac gggagacggg
      421 gtatgtacca gatggaaaaa atcacaacgg gtgtgtcata caccacgtca gcggtgggga
      481 cgggatactg gttactgcag ctgctggaca aagtctctcc gtcccagtgg gtggcaatcg
      541 gtgtgctggg gagtctgctg tttggcctgc tgacgtatct tactaacctg tatttcaaaa
      601 tcagagagga ccgtcgtaag gcggcgcggg gaaagtaaag cgatgaagaa aaaatacgaa
      661 ctggttgtta aagggataaa taattacccg gataagatta ctgttactgt ggcactggaa
      721 attggtgggt atccgtcact gttgttgcca gatgtggcga ttagtcttga ccgtactgaa
      781 ggtgccacgc tggagtttta cgaagctgag gcgaaaaagc aggcgaagca gtttttcatg
      841 gatgttgctg ccgggttatg tgaaggggat ggtccgttgc cggaaaagcg gcccatcatt
      901 ttagaggcgc aggatgtgtt gataacctac agaggaaaac taccgggaat aattactggt
      961 tctctgaaga ctccaccgct ggcctgaaga cttaatatat ccagggattt gaaatcgata
     1021 aaccctgata aatatccatg aacgcaaaaa tcagatacgg cctgtcggct gccgttctgg
     1081 cgctgattgg tgcaggggcg tctgcgcctg aaatcctcga ccagtttctt gacgaaaaag
     1141 aaggtaacca caccacggca taccgtgatg gtgcggggat ctggaccatc tgccgcggtg
     1201 ccatcctggt ggatggtaaa cctgtcgtcc cgggcatgaa gttgtcgaag gaaaaatgcg
     1261 accaggttaa cgccattgaa cgtgataagg cgctggcatg ggtggagaaa aacatcagag
     1321 tgccactgac cgaaccccag aaagcgggga ttgcgtcatt ctgtccgtac aacattggcc
     1381 ccggtaagtg tttcccgtcg acgttttaca gacggattaa tgccggtgac cgcaggggag
     1441 catgcgaggc gattcgctgg tggattaagg acggtggcag agactgccgt attcgctcaa
     1501 acaactgcta cggtcaggtc tcacggcgtg accaggagag cgcgctggcg tgctggggaa
     1561 ttgacagata agcagaatat tttgctgaaa aatgcgattt gctcacgcgg acggataaca
     1621 cgaaatcctg cgaactgaca aaaactaagt gaataaaagt aaaaaccccg tttgttggct
     1681 gcaagcgggg ttttgtgttt cctgactccg gaaaagtcaa aggagaaagt gtgtttgatt
     1741 ttagcaaact gattcgggag attcgaatga tggctgaaaa attatccacc tggaagttca
     1801 tccttatctg gctggtgttt gtgattatgg cttccggtta tttcattggt cagatacgct
     1861 ggtggtgaaa tgaaccgcgt actgtgcgtg gtcatcattg ccctgctggt ggcctgtggt
     1921 gcgcttagtc tggggctgaa tcattaccgt gataacgcca taacctacaa agagcagcgc
     1981 gataaaaaag tcagtgagct ggagctggca aatgcaacca ttactgatat gcagcagcgc
     2041 cagcgtgatg ttgctgcact tgatgccaga tactcgaggg aattagccga tgcgagagct
     2101 gaaaatgaaa ctctgcgcgc tgatgttgcc gctggtcgta agcgcctgcg gatcaacgcc
     2161 acctgccccg gtaccgtgcg tgaagccacc ggcacctccg gcgtggataa tgcaaccggc
     2221 ccccgactgg cagacaccgc tgaacgggat tatttcatcc tcagagaacg
//
LOCUS       DAZFRJ010000364         2247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000364 DAZFRJ010000000
VERSION     DAZFRJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.367"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..74
                     /gene="dicB"
                     /locus_tag="ACHKUN_004871"
                     /gene_synonym="ftsT"
     CDS             <1..74
                     /gene="dicB"
                     /locus_tag="ACHKUN_004871"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6662962.1"
                     /translation="LLNKICIVSMLARLRLMPKGCAQ"
     gene            71..262
                     /gene="ydfD"
                     /locus_tag="ACHKUN_004872"
     CDS             71..262
                     /gene="ydfD"
                     /locus_tag="ACHKUN_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO6662963.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            356..>2247
                     /locus_tag="ACHKUN_004873"
     CDS             356..>2247
                     /locus_tag="ACHKUN_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6662964.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGH"
BASE COUNT          686 a          513 c          559 g          489 t
ORIGIN      
        1 agctattaaa taaaatatgc attgtttcaa tgctggctcg tttacgtctg atgccaaaag
       61 gatgtgcaca atgaattcag catttgcgct tgttctgaca gtttttcttg tttccggagt
      121 gccagttgat attgcagtca gtgttcacag gacaatgcag gagtgtatga ctgcagcaac
      181 cgaacagaaa attcccggta actgttaccc ggtcgataaa gttattcacc aggataatat
      241 cgaaatcccg gcaggtcttt aaaacagttc cgtaataaat atccggtttc attcttatat
      301 gccagcaatg gcagggattt gttcatcctt aaatctgtca tgaggttaaa acaaaatgag
      361 taaagtcttt atttgcgccg ctattcctga cgaactggca acaagggaag aaggctctgt
      421 ggctgtagcc acagccattg aagctggcga cgaacgccgt gctcgagcaa aatttcactg
      481 gcagttcctg gaacattatc cggctgctca ggactgcgct tataaattta ttgtctgcga
      541 ggataaacct ggcatacccc gccctgccct cgattcatgg gatgctgaat atatgcagga
      601 aaaccgctgg gatgaggagt ctgcttcttt tgtcccggtt gagactgaat ccgatccgat
      661 gaacgtcact tttgacaagc tggcccctga agtacagaac gctgtcatgg ttaagttcga
      721 cacatgtgaa aacatcaccg ttgatatggt tattagcgca caggaattgt tgcaggaaga
      781 catggcaaca ttcgacggac atatcgttga agcgttgatg aaaatgccag aagttaacgc
      841 catgtatccg gagcttaagt tgcacgccat tgggtgggtt aagcataaat gtattcctgg
      901 tgctaaatgg cccgaaattc aggcagagat gcgcatctgg aaaaaacgtc gcgaaggtga
      961 acgcaaggaa accggaaaat acacgtctgt tgttgatctc gcccgcgcca gagccaatca
     1021 acagtacact gaaaattcaa caggaaaaat cagcccggtc attgctgcca ttcatcgcga
     1081 atacaagcag acatggaaaa cactggatga cgaactggcc tacgctctct ggcctggtga
     1141 tgtggatgcc ggaaacattg acggcagcat ccatcgctgg gcaaaaaaag aagttatcga
     1201 caacgaccgc gaagactgga agcgtatctc ggcatcaatg cgcaaacagc ctgatgccct
     1261 tcgctacgac cgccaaacta tttttggcct tgtccgtgag cgtccgatcg acattcacaa
     1321 agatcccgta gcactgaaca aatatatctg cgaatacctg acgacaaagg gcgtgtttga
     1381 gaatgaagaa acagacctgg gcactgttga tgttctccag tcatcagaaa cacaaactga
     1441 tgcagtggaa actgaggtat ctgatatccc aaaaaatgaa accgcgccgg aagctgaacc
     1501 atctgtagag cgtgaggggc cgttctattt cctcttcgca gataaggacg gagaaaaata
     1561 cggtcgcgca aacaaacttt ctggtctgga taaggcactg gctgctggcg ccactgaaat
     1621 cacaaaagaa gaatattttg cccgaaaaaa tggcacatac acgggcttac cgcaaaatgt
     1681 agataccgct gaagattcag aacaaccaga gccgataaaa gttaccgctg acgaagtaaa
     1741 caaaattatg caggcagcca atatcagcca gcctgacgcc gataagttgc ttgctgcatc
     1801 acgtggtgaa tttgttgaag ggattagtga cccgaatgat ccgaaatggg ttaaggggat
     1861 ccagacccgc gattctgtga accagaacca gcatgaatcg gaacggaact accaaaaagc
     1921 ggaacaaaac agtccaaatg cgttacaaaa cgagccagaa acgaaacagc ctgaaccagt
     1981 ggcgcaacag gaagtggaaa aagcctgcac cgcctgcggt cagaccggcg gcggcaactg
     2041 ccctgattgt ggcgcggtga tgggcgacgc aacataccaa gaaacattcg atgaagagta
     2101 tcaggttgaa gttcaggaag atgatccgga ggaaatggaa ggcgctgaac atccacacaa
     2161 ggagaacact ggcggcaatc agcatcacaa tagcgataat gaaactggcg agacggcaga
     2221 tcacccaatt aaggtgaacg gtcatca
//
LOCUS       DAZFRJ010000365         2246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000365 DAZFRJ010000000
VERSION     DAZFRJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.368"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            444..659
                     /locus_tag="ACHKUN_004874"
     CDS             444..659
                     /locus_tag="ACHKUN_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6662965.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            664..1008
                     /locus_tag="ACHKUN_004875"
     CDS             664..1008
                     /locus_tag="ACHKUN_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO6662966.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1059..1592
                     /locus_tag="ACHKUN_004876"
     CDS             1059..1592
                     /locus_tag="ACHKUN_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6662967.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGTTRVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDLKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1748..1930
                     /locus_tag="ACHKUN_004877"
     CDS             1748..1930
                     /locus_tag="ACHKUN_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662968.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1943..2074
                     /locus_tag="ACHKUN_004878"
     CDS             1943..2074
                     /locus_tag="ACHKUN_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662969.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            2082..>2246
                     /locus_tag="ACHKUN_004879"
     CDS             2082..>2246
                     /locus_tag="ACHKUN_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6662970.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQ"
BASE COUNT          590 a          452 c          632 g          572 t
ORIGIN      
        1 cataataaaa aaagagccag cgactgacct gaaaaagaag acgctggcta aaaggcctta
       61 tatgtttgta gagacttatt tttcacagac agcaatgatg cctgtcaata tattatcaat
      121 atgcggattg tttcagttac agatgcttta ttaaggaaaa aaacagccag cactgacttt
      181 cggtggggag gtgctggctc aaaaggatag ttggatttca catgatactt atgcctggcg
      241 gtatattttc tgacagacag tgacggatgt tgtcaagata ttgtgtcatt tataacctga
      301 accagggggg ccggaatgtt atctggcatt tttagcagag cctgaatgcc ataatcacgg
      361 ctcccggagt tggccgtcag tgagtgacac tggcggcttt ttgttttcct ttactttcat
      421 tttctgtcgg cggtgacgga gacatacatc agatggaaaa aatcacaaca ggtgtgtcat
      481 acaccacgtc agcggtaggg acgggatact ggttactgca actgctggac aaagtctctc
      541 cgtcccagtg ggtggcaata ggtgtactgg ggagtctgct gtttggcctg ctgacgtatc
      601 tgactaacct gtatttcaaa atcagagagg accggcgtaa ggcggcgcgg ggagagtaaa
      661 gcgatgaaga aaaaatacga actggttgtt aaagggataa ataattaccc ggataagatt
      721 actgttactg tggcactgga aattggtggg tatccgtcac tgttgttgcc agatgtggcg
      781 attagtcttg accgtactga aggtgccacg ctggagtttt acgaagctga ggcgaaaaag
      841 caggcgaagc agtttttcat ggatgttgct gccgggttat gtgaagggga tggtccgttg
      901 ccggaaaagc gccccgtaat tttagaggcg caggatgtgt tgataaccta cagaggaaaa
      961 ctaccgggaa taattactgg ttctctgaag actccaccgc tggcctgaag acttaacata
     1021 tccagggatt tgaaatcgat aaaccctgat aaatatccat gaacgcaaaa atcagatacg
     1081 gcctgtcggc tgccgttctg gcgctgattg gtgcaggggc gtctgcgcct gaaatcctcg
     1141 accagtttct tgacgaaaaa gaaggtaacc acaccacggc ataccgtgat ggtgcgggga
     1201 tctggaccat ctgccgcggt accacccgag tggatggtaa acctgtcgtc ccgggcatga
     1261 agttgtcgaa ggaaaaatgc gaccaggtta acgccattga acgtgataag gcgctggagt
     1321 gggtggagcg caatattaaa gtaccgctga ccgaacccca gaaggcgggt atcgcgtcat
     1381 tttgtcctta taacattggc cccggtaagt gtttcccgtc tacgttttac agacggatta
     1441 atgcaggtga tctaaaaggt gcctgcgaag cgattcgctg gtggattaag gacggtggca
     1501 gagactgccg tattcgctca aacaactgct acggtcaggt ttcacggcgt gaccaggaga
     1561 gcgcgctggc gtgctgggga attgacagat aagcagaata ttttgctgaa aaatgcggtt
     1621 tgctcacact ggcggataac acgaaatcct gcgaactggc aaaaactaag tgaataaaag
     1681 taaaaccccg tttgttggcc gcaaacgggg ttttgtgttt ctgaccttga gtaaggcaag
     1741 ggagaacatg ggaaagtata aacgaattct gttgagattg actatgaaaa acggccttga
     1801 actgaaagcg cctgtaactg atgacgtcag cagagcgctg gcttttgcta ttaagtgggt
     1861 ggcggtcggt attgctgtgt ctccgatgct gtatgggctg gcaaaactgg tcattgcgtt
     1921 gaaatcgtga agaggattaa gcatgtcaga caagctcata acgctggcga agatcctctg
     1981 tgtaatcgtc ggcatttcat tttcactaat gctggttgct atttgctttt ccactgcctg
     2041 gcgcgtctta actttgtcat ggctggtggg gtgaggggga tatgaaccgt gttctgtgtg
     2101 tggtgattat tgtcctgctg gtagcctgtg gtgtgcttag tctggggctg aatcattacc
     2161 gcgataacgc cattacctac aaagcgcaac gcgataaaaa agccagtgag ctgaagctgg
     2221 cgaacgtgac aattactgat atgcag
//
LOCUS       DAZFRJ010000366         2237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000366 DAZFRJ010000000
VERSION     DAZFRJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.369"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2237
                     /gene="agn43"
                     /locus_tag="ACHKUN_004880"
                     /gene_synonym="flu"
     CDS             <1..>2237
                     /gene="agn43"
                     /locus_tag="ACHKUN_004880"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO6662971.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIA"
BASE COUNT          547 a          604 c          693 g          393 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgc
//
LOCUS       DAZFRJ010000367         2219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000367 DAZFRJ010000000
VERSION     DAZFRJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.370"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..253
                     /locus_tag="ACHKUN_004881"
     CDS             <1..253
                     /locus_tag="ACHKUN_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6662972.1"
                     /translation="RHIWLRDAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWT
                     LCTECHRQKSVSETASGMPDPTLPEVSGGHGRADDIIGL"
     gene            545..1108
                     /locus_tag="ACHKUN_004882"
     CDS             545..1108
                     /locus_tag="ACHKUN_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6662973.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1105..>2219
                     /locus_tag="ACHKUN_004883"
     CDS             1105..>2219
                     /locus_tag="ACHKUN_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6662974.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVTGR"
BASE COUNT          501 a          556 c          699 g          463 t
ORIGIN      
        1 gcgtcatatc tggctcaggg atgccgggca gtgctgtctc tgtggtcgtg tggttgacct
       61 ctgtgacagt gaactcgatc accgaattgc acttcagttc ggtggtggta atgaggagac
      121 gaatctctgg acgctctgta ccgaatgcca tcgccagaag tcagtcagtg aaacggcgag
      181 tggtatgccg gacccgacgc tgccggaggt gtccggaggt catggcaggg cagacgatat
      241 catcggactg tgacccgccc cggggggggg gatcatccgg cgaaaaaaac gatcgccccg
      301 gacaccgccc cccgtctcac gcagagaaaa aattcccgtt tcaggccagt taacatgtta
      361 actggctgtc cgggcatttt tgcggttttt atctttatta ttcagtttgt tgcgtggaaa
      421 aaatgttaac aggctttttc agcaaatgtt aaccaggcag cagttaacat ttgcggcatg
      481 agacgccggg aaaaatgggc tgaaccatac ccggctgagt gcgttatgga cccgggagga
      541 ggctgtgctg acaacgcaaa aacgaaaatt tgcgctggcg ctcatgtccg ggaaaaacaa
      601 aacagcgtca gccattgccg ctggttattc ggcgaagacc gccagggtta aaggctcgca
      661 gctggcaaaa gatccggagg tgcttgcgtt tatagcccgt aaacaatgcg agacggtgga
      721 ggtggatgag gttcctgttt accggcagaa aaaatcagag ccggaggata aaccccgtcg
      781 ccgtgaggcg gctgcaatac cacagccgga cgaaacaaat ccggagatgc caccgcccgc
      841 ggtgatgtct cctggtattg aatatatgga ggacggtctt cccgatccgg tgaaagcgat
      901 ggggcgtctt ctggtggaga acattaatac cgaccccagg ctggcgctgg atgcggctta
      961 taagctggcg cagttcacgc accacaaaaa aggggatgcc ggtaaaaaat cggcaaaagg
     1021 tgacgcggcg aaaaaagcgg ctaaccgttt tgcggtgcca ccaccacccc gcctggtggt
     1081 gaataatgat aatgagggca acggatgata cctgtgtgga gcacggcctg cccggactgg
     1141 gcagagcgcc tgaaaaaggg gctgtcgatt attccggctc cgatttatcc ggaccaggct
     1201 gcacatgcac tggcgatttt taaacaactg cgaattgtgg atgcaccggg tagcccgaca
     1261 ttcggggagt cctgtgcacc gtgggtgttt gacctggtgg cggccctgtt tggctcctac
     1321 gatgcgcaga ccggtgttcg ccatatcaag gaagtgttta tccttatccc caagaaaaac
     1381 agtaagtcca cgctggctgc ggggatcatg atgacggcgc tgttactgaa ctggcggcag
     1441 gcggcgggct acaccattct ggccccgacc gtggaggtgg cggctaacgc cttcaaccct
     1501 gccagggata tggtacgacg ggacgatgat ctggatgacc tctgtcaggt gcagacacat
     1561 atccggacca tcacccatcg ggtgacggac accaccctga aggtggtggc agccgatccg
     1621 aatacggtgt ccggtatcaa gtccgtgggg acactgattg atgaactgtg gctgtttggc
     1681 aagcagtaca aggcggaaga catgctacgt gaagccatcg gcgggctggc ctcccgtccg
     1741 gaagggtttg tggtgtatac gaccacccag tcgaatgaac cgcccgccgg ggtgttcaga
     1801 cagaaactgc agtatgcccg ggatgtccgt gacggcaaaa ttcatgatcc gcactttctg
     1861 ccggtgattt ttgagcatcc tcctgaaatg gtggaaagcg gggctcacct gctgatggaa
     1921 aacctcgcca tggttaaccc gaatctcggt tattcggtgg atgaggcctt tctgtaccgg
     1981 gagtaccgta aagcccggga ggctggtgag gaagcatttc gtggcttcat gtcaaaacac
     2041 gccaatgtgg aaatcggtct tgccctgcgt tctgaccgct gggcgggcgc ggatttctgg
     2101 gagcagcagg gcaggcgcgt cagcctggac gatatcctgc agcgcgctga tgtggtgacg
     2161 gtggggattg acggcggggg cctggatgat ctgctgggaa tgtacgtgac tggccgtga
//
LOCUS       DAZFRJ010000368         2210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000368 DAZFRJ010000000
VERSION     DAZFRJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.371"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..563)
                     /gene="yjjX"
                     /locus_tag="ACHKUN_004884"
     CDS             complement(42..563)
                     /gene="yjjX"
                     /locus_tag="ACHKUN_004884"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO6662975.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            615..1262
                     /gene="gpmB"
                     /locus_tag="ACHKUN_004885"
     CDS             615..1262
                     /gene="gpmB"
                     /locus_tag="ACHKUN_004885"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO6662976.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(1259..2128)
                     /gene="robA"
                     /locus_tag="ACHKUN_004886"
     CDS             complement(1259..2128)
                     /gene="robA"
                     /locus_tag="ACHKUN_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO6662977.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
BASE COUNT          534 a          593 c          595 g          488 t
ORIGIN      
        1 gtgcaccgaa tgccggatgc ggcgtgaacg ccttatccgt cctacaatgc ctgatacacg
       61 gcattatgaa acggactcag cgccaggatc accgcctggt gatagacgct ggcgcgggtg
      121 agtttcccgg cggtaaacac gccgatcgcc ccttccttac gaccaatctc atcaataccg
      181 gtataacgcg acatcacggg gccgagcgcc tcgccttcac gcactttttc cagaatcacc
      241 gcaggcaacg gcaaagtagc cgaacgcgcc tcgccgcgct ggctggcgtt ttcaatcacc
      301 acccaactga aagtgctgtc accatcgatg ccagcttcaa tcgccaccca aaaatcagcc
      361 tctggaagta aacggcgggc atttgctacc cgatttcgtg cgccagcgcg cgtttcctca
      421 ctgccaaagg gttgttccgg tacaccgctc tcgacggcaa cggatgcaat atggcaggat
      481 ccttcgccga agatctcgtg aaatgcctgc agaatggcct gaattttagc gggattggtg
      541 gtcgcacaga caacttggtg cataatcagc attactcaga aaattaacgt tacagcagta
      601 tacggaaaaa aagcatgtta caggtatacc tagtccgcca cggtgaaacg cagtggaacg
      661 ccgagcgacg tattcagggc cagtctgaca gcccgctgac cgccaaaggt gagcaacagg
      721 cgatgcaggt ggcaacccgt gccaaagagc ttggcattac gcatatcatc agtagcgatt
      781 taggacgcac ccggcgtacg gcggaaatca tcgcccaggc ctgcggctgc gacatcatct
      841 ttgattctcg cctgcgtgaa ttaaacatgg gtgtgctgga aaaaagacat atcgattctc
      901 tgaccgaaga agaagagaac tggcgtcggc agctggtcaa tggcaccgtt gacgggcgta
      961 ttcctgaagg cgagtcaatg caggagctca gcgatcgcgt caatgcggca ctggaatctt
     1021 gccgggactt accgcaggga agccgaccgc tgctggtgag tcatggtatt gcactgggat
     1081 gcctggtgag tacgattctc ggattaccag catgggcaga gcgccgctta cgtctgcgta
     1141 actgttctat ttcgcgcgtg gattatcagg aaagcctgtg gctggcgtct ggctgggttg
     1201 ttgaaaccgc aggggacatc tcgcatctgg acgcccctgc attagatgag ttgcagcgtt
     1261 aacgacggat cggaatcagc agttcacagc gcagattaat tgggcgatct cctgctttgg
     1321 catcttctgc cgggtagtat cgctcaatat cctgaccttt acggcgcgtc aggttaagca
     1381 ttggcatgca tgttccatat accgtcagga taaactcctg cacgccggtt cccagacctt
     1441 cataggtaaa catcacatat tcgccgccct gcagcatcac cggatgcccc gtcagtacat
     1501 agccatctgc ctgatcctgg gctaacgcgg tggtatagaa tacttcttgt tcgtcgtctt
     1561 tatcctgact cggacgcgtt tcattcaggc cgtagagcac cggcggaatg gtcggcgcgt
     1621 tgccgagaaa atcgtgccag aactgataac gcatttcatg gcggaaatca gagatttgct
     1681 ccagcgaaca ggagtagctc tgggtaacac caatcagcgg cgtatcttcc agggtgacaa
     1741 atttgtgctc tggcatagtg aattcaccca gacgtagcgg cgggcgaata ccaaaggcgc
     1801 tccattcagg agaacggcgg taaagtgcag gagtctgggc aaactgcttc ttgaatgcgc
     1861 gggtaaatgt ctgttgagag tcgaagcggt attgcagcgc gatgtccaga atcggacgcg
     1921 cagtcaggcg tagtgcgacc gccgatttcg acaaacgacg agcacgaata tacgcgccaa
     1981 tagcatggcc agtgacatct ttaaacattc tctgtaagtg ccacttggaa taacctgctt
     2041 tcgccgctac attgtcgagc gacaggggct gatccagatg accttccagc cagattaaaa
     2101 ggtcgcgaat aatgccggcc tgatccataa aatatcctca tcctttcaac aacgagcacc
     2161 tgacatcagg taattggata atagcatttt ttgctgtttt agcattcagt
//
LOCUS       DAZFRJ010000369         2199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000369 DAZFRJ010000000
VERSION     DAZFRJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.372"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..250)
                     /locus_tag="ACHKUN_004887"
     CDS             complement(<1..250)
                     /locus_tag="ACHKUN_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HFO6662978.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKL"
     gene            complement(367..1205)
                     /gene="aadA1"
                     /locus_tag="ACHKUN_004888"
                     /pseudo
     CDS             complement(367..1205)
                     /gene="aadA1"
                     /locus_tag="ACHKUN_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            1354..>2199
                     /gene="intI1"
                     /locus_tag="ACHKUN_004889"
     CDS             1354..>2199
                     /gene="intI1"
                     /locus_tag="ACHKUN_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO6662979.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHS"
BASE COUNT          493 a          612 c          589 g          505 t
ORIGIN      
        1 caagcttttg cccatgaagc aaccaggcaa tggctgtaat tatgacgacg ccgagtcccg
       61 accagactgc ataagcaaca ccgacaggga tggatttcag aaccagagaa agaaaataaa
      121 atgcgatgcc ataaccgatt atgacaacgg cggaaggggc aagcttagta aagccctcgc
      181 tagattttaa tgcggatgtt gcgattactt cgccaactat tgcgataaca agaaaaagcc
      241 agcctttcat gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat
      301 aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg
      361 cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg
      421 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
      481 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
      541 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
      601 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
      661 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
      721 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
      781 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
      841 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
      901 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
      961 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     1021 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     1081 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     1141 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     1201 ctcatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     1261 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     1321 gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     1381 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     1441 agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt
     1501 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     1561 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     1621 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     1681 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     1741 gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag
     1801 ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat cgtgcgggag
     1861 ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc cagcctgcgc
     1921 gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg ccgcagcggc
     1981 gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc ctggccgtgg
     2041 ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt cgtgcgtcgc
     2101 catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga acaagcaggc
     2161 atcacgaagc ccgccacacc gcacaccctc cgccactcg
//
LOCUS       DAZFRJ010000370         2194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000370 DAZFRJ010000000
VERSION     DAZFRJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.373"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            341..1093
                     /locus_tag="ACHKUN_004890"
     CDS             341..1093
                     /locus_tag="ACHKUN_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO6662980.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            1090..1644
                     /locus_tag="ACHKUN_004891"
     CDS             1090..1644
                     /locus_tag="ACHKUN_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO6662981.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1646..1918
                     /locus_tag="ACHKUN_004892"
     CDS             1646..1918
                     /locus_tag="ACHKUN_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6662982.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          647 a          517 c          528 g          502 t
ORIGIN      
        1 gtgaggagaa aagatccagt aataaactga aataaatatc gcgacttata tgaaaaagaa
       61 atatatgttt aattttatct ctgtgaacag tcatgaacag tagatgatca ctttaattaa
      121 aacactacac cacttaacca catgtattta ctatattttt attgaagtga acagtagtga
      181 atagtatgta ttaaaggaag cgtgtagatc agacatgaca cctttctctg gctagccaga
      241 acaaggtcat tgttcagtca ctgacacaat cctcctcggt agtgcgcttg tatggacccc
      301 ggcacaattg acacaacaac aaaacactac cggagcagtc atgactactg ttaaccagat
      361 ccctgatgca aacattacac cctccctccc accgaaaatt cgcgaagcgg tggataaagt
      421 taaggcagca aaagctgtct ggcaggaaga acgacgaaaa caaactgaag ctgctgcaat
      481 gattgaaact atccgtaaac gtcaggaaga tacaaaaacg gaaacgcagg cgcttaatga
      541 tgaatggcga aacctgtttc gtgagaatca gggaaatatg acgccacgca tgaaaaaact
      601 gcgggcagaa atcgccctcg gacgcgaaac actggatgag ttcgaagatt tgctggcagc
      661 tcaggcggca gaaaatgaat tcctgccctg gaaaactgcg gatgctgcaa accactacat
      721 cagcgaacat aatcgcctga ttgaaactca tgcagtgtgg ctctggaatg agtttatgaa
      781 ggaacacggt cagaaactta ttcagatcct tggactgctg aaaatgactc tgggccgaag
      841 cgcttctgct gttatcggtg tagttcatac cgtaaacgac cccgaaagtg tgctgaagca
      901 atttatcagc gagcaactca ccactccggc actgtcctgt aacgtatctt caaccgatga
      961 tattaccctg ccggggatca gcatttatgc ggacgataaa gccatccagg atgccagaca
     1021 atcccccagc cctgcagcgc gttcacggat gcttaaacag cgtgacatgg ttaaaggggg
     1081 cgagaagaaa tgaatgccgg aaccattact caggaagcac tcaacgatta ccgctcggcg
     1141 ataaaaagct ggctgacact acgtaatacg caaagtacct accagcttcg tctggcggct
     1201 ctgctggata ctgaagaaaa accggcagca tatgccagcc agcttgagga tcttcgtgag
     1261 cgtcttgcag tcctcgaatg gcagattaac tgtgctgcac gggatggtct ttatgctcat
     1321 cagattgtgc tggagagttg tgttacaagc gcaacggaaa acttcatgag cgagcatggt
     1381 gatgcactca ctgacgctct ggctcctttt ctttgcgctc catacgggct tgaggcggca
     1441 atgaaaatat tacgaaccgc tgtagcccga caaacggaaa tgcgtactcc ggtaatttca
     1501 gcagcatata agagcattat cgacgaaacc ggattaacgg tggatgcatc aatgcgcgct
     1561 gatgcttcag ccagtttcac cccggcacaa cataaagttt tcctggcccg ccttaaccga
     1621 cttaatgaaa aaggagtgtg ctgatatggc cctgaagtgt cctgaatgtg gcgcggtcgc
     1681 tcacgccaga accagcgcct atgaagctcc gtcggttaaa cgctcatggt atcagtgcca
     1741 gaatctggaa tgttcctgca catttaccgc actggaaagc gtggatacga taattatgaa
     1801 gcctcggtgc aatgaacagg aatcagacaa agcaaaaata ccggaaaaac aacagcaaac
     1861 tctcaatcgc tatggctccg cgtcaaagct gtcaaaccgt cagcaaattc ctgtctgatt
     1921 agcaaaatac gaccacgaag tcccggtcca gtaccgggat tttttacgcc tttttcctgg
     1981 ctggcctgag agcgtatgag tgcatatcta tggcgcatga aaacgcatga gtctcatgcg
     2041 ccatttttga cgcgaaagcc cttgtgtggt ggcttctgag acgatttacg gggtgcatga
     2101 aaaccagtct gttaagcgaa gcgggcaggc gggcggggca ttgcgcgcag gaatgtgtat
     2161 accgttttag taatgatcgc tgacatctca cttt
//
LOCUS       DAZFRJ010000371         2180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000371 DAZFRJ010000000
VERSION     DAZFRJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.374"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHKUN_004893"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHKUN_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO6662983.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKUN_004894"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKUN_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6662984.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..>2180
                     /locus_tag="ACHKUN_004895"
     CDS             1077..>2180
                     /locus_tag="ACHKUN_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO6662985.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRI"
BASE COUNT          512 a          616 c          594 g          458 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccggacagc ccggctgtgg gtctacgtcc
     1921 gtgatgaccg taacgccggt tcacagatgc ccccggcggt ctggttcgcg tacagtccgg
     1981 accggaaagg tatccatcca caaaatcacc tggccggtta cagcggtgtg cttcaggccg
     2041 atgcttacgg tggttaccgg gcgttatacg aatccggcag aataacggaa gccgcgtgta
     2101 tggctcatgc ccggagaaaa atccacgatg tgcatgcaag agcgcccacc tacatcacca
     2161 cggaagccct gcagcgtatc
//
LOCUS       DAZFRJ010000372         2175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000372 DAZFRJ010000000
VERSION     DAZFRJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.375"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..802)
                     /locus_tag="ACHKUN_004896"
     CDS             complement(113..802)
                     /locus_tag="ACHKUN_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6662986.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHKUN_004897"
     CDS             complement(817..939)
                     /locus_tag="ACHKUN_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6662987.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            1278..>2175
                     /locus_tag="ACHKUN_004898"
     CDS             1278..>2175
                     /locus_tag="ACHKUN_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO6662988.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVT"
BASE COUNT          687 a          478 c          400 g          610 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagttc
     1021 ttatattgct atatagaata gccattacta atgcttttaa atttaataaa ataaaaatta
     1081 taaaaaacat aaaacaccac gcaaacacac ttaataaaaa caccgttaca ttaaaagata
     1141 ataaaaaccg caataaaaaa cgaataaatc aattgtctca cgcaattata aaacatcata
     1201 ttgattacgc accttgtatt acaaactcat gtatgtaaaa tacgcgcacc attcaaaaaa
     1261 aaggaagaca ataacatatg aaaaaaagtg tcatcgctgg cgtctttatt gctctgtcat
     1321 ttaccacgtg ttcagctatc gcgaacagcc ttgcattatc attagcaaat gatgatgcag
     1381 ggaagtttca accaatactt aatgatattt atggcaataa acatgaaaac agagatgatt
     1441 actcacaagg cttatttctg ggatatagcc acgatatctc agactcgagc caattatctc
     1501 tccatattgc gcaagatatt tactctccat caggcagtaa taaaagacac aacacagctg
     1561 taactggaga cagagctttt agtgcataca ctcacactgg tattgaatgg aactcccttg
     1621 cgaatgactg gattcgctat cgattaggta ctgacatagg tgttgttggc cccgacgcag
     1681 gcggtcagaa agtacaaaat aaagctcatg agattattgg ggcagaaaaa tatcatgcat
     1741 gggatgatca aatagagaat cgctacggtt atactgtaaa agggatgcta tccatgacac
     1801 caagtatgga tattttaggt gctaatgttg gattataccc tgaagtttct gctgttactg
     1861 gaaacttatt tcaatatgta gcatatggcg caaccattgc cattggtaat gataaaacct
     1921 tcaattcgga taatggcttt ggtctgctgg ctccccgtgg tttaatgcat atgtccgata
     1981 caagcggatt caaatacaag atttttgcag gtatggaaag acgagatgtc aatcgcaact
     2041 atactctcga aggaaaaaca atacagacga aacaaacaac agtatcgcta aacaaaactg
     2101 ttgatgaata tcaagttggc gcaacaattg ggtatgcacc tgtagccttc acactagcat
     2161 ttaataaagt aacat
//
LOCUS       DAZFRJ010000373         2164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000373 DAZFRJ010000000
VERSION     DAZFRJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.376"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHKUN_004899"
     CDS             <1..108
                     /locus_tag="ACHKUN_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6662989.1"
                     /translation="SKESCLQARDEQKISGECLPVKKVSLYLNNETPAG"
     gene            201..>2164
                     /locus_tag="ACHKUN_004900"
     CDS             201..>2164
                     /locus_tag="ACHKUN_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO6662990.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSVQEKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVAREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLARDTDKVFPNPGNSDLGLITA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTFDTLD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEIVLKRESPFKEWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTAARHTPEKDILEEVNRELA
                     AKQETEEEENDEEKPQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKY
                     QKLRAELHEARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQN
                     QPETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVM
                     GDATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITV
                     NGHRIITSTSRTCDHLMIDLETMGKNPDAP"
BASE COUNT          671 a          539 c          527 g          427 t
ORIGIN      
        1 agtaaagaat cctgcctcca ggcaagagac gagcaaaaaa tttctggtga atgcctcccg
       61 gtaaaaaaag tatcgctgta cctgaataac gaaacaccgg ctggataacc ctccagccat
      121 attaacacca taccaacgga ttaaaaatgc cagcaatggc agggatttgt tcacccttaa
      181 atctgtaatg aggtttatca atgagcactg ataaagaaga atttgcgcta tattgcgaag
      241 caaaaaatga caaagtcaga aaacgtctgg gaattaaagg tggtttttac tggactacag
      301 caaaaaaatt atctgttgcc atctcccgct gcattaccgc aatggatgac aacgattatg
      361 atgaagacga ctttaaaaaa cccgttcgcg tccatttacc cgttgtgaat gaccttccac
      421 ctgaaggcgt gtttgatacc gaattctgca accgatacga aaaaggcggg gaagatggca
      481 ttacaatggt atttatcgcg ccctctccct ccgtgcagga gaaaccagcc agtactgaca
      541 ataccaacgt caacggcgaa gacatgacgg agattgagga gaatatgctc ctgccggttt
      601 ctggtcagga gctgcccatt cgctggcttg cgcaacacgg cagcgaaaaa ccagtaacgc
      661 acgttgcacg ggaagaactt caggcattac atatagcacg ggcggaagaa ctgccggctg
      721 ttactgccct ggccatttcc cacaacacaa agctgctcga cccgctggag attcgcgacc
      781 ttcataaact ggcacgcgac actgacaaag ttttccctaa tcccggtaat tcagacctgg
      841 gactgataac tgcttttttc gaggcatacc tggacgctga ctacactgat cggggtctgc
      901 tgacaaaaga gtggatgaaa ggaaatcgtg tttcgcgtat cacccgtacg gcttccggtg
      961 caaatgccgg tggcgggaac aaaaccgatc gcaatccgaa tttagtacac accttcgata
     1021 cgctggatgt ggagattgca gcagccacac ttccgatgga ttttaatatt tatgaaattc
     1081 cgggcagcgt ttatcgtcgc gcaaaagaaa tcgtcctgaa aagagaaagt ccgttcaaag
     1141 aatggtccgc agcacttcgc gcaaccccgg gtattctgga ctattcccgc gccgctattt
     1201 ttgcacttat ccggagcgct caccctgagt tttatcacta cccgggacgc cttcaggggt
     1261 atatcaacgc ctacttaacg gagactgatc acgagaaccc cagcaaggaa actctcactg
     1321 ctgcacgaca tacaccggaa aaagatatcc tggaagaagt taaccgcgaa ctggctgcta
     1381 agcaggaaac agaagaagaa gaaaatgatg aagaaaaacc gcaaccatct tgcgcaatgg
     1441 cagatgaaca ggcaacggct gaaacagtgg aaccggatgc aactgaacat catcaggaca
     1501 cgcagccgct ggatgctcag tcacaggtaa attctgttga tgcgaaatat cagaaactgc
     1561 gggcagaact ccatgaagcc cggaaaaaca ttccgcccca aaatcctgtc gatgcagaca
     1621 aattactggc tgcctctcgc ggagaatttg ttgaagggat tagcgacccg aatgatccga
     1681 aatggattaa ggggatccag acccgcgatt ctgtgtacca gaatcagcca gaaacggaac
     1741 agaacgacca gaaagcggaa cagaacagcc caaatacgca acaaaacgag ccagaaacga
     1801 aacaacctga accagtagtg caacaggaac cggaaaagat ctgcaccgcc tgcggtcaga
     1861 ggagtggcgg caactgccct gattgtggcg cggtgatggg cgacgcaaca taccaggaaa
     1921 cattcgatga caagaacctg gttgaagttc aggaagacga ttcggagaaa atggaaggcg
     1981 ctgaacatcc acacaaggag aatgctggca gcgctcagga ccacgccagc gatagtgaaa
     2041 ctggcgagac ggcagatccc ttaattacgg tgaacggtca tcgcattatc acatccacca
     2101 gcaggacgtg tgaccatcta atgatcgacc ttgaaaccat gggaaaaaat cctgatgccc
     2161 cgat
//
LOCUS       DAZFRJ010000374         2132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000374 DAZFRJ010000000
VERSION     DAZFRJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.377"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..467
                     /locus_tag="ACHKUN_004901"
     CDS             <1..467
                     /locus_tag="ACHKUN_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662991.1"
                     /translation="AVYARLHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFF
                     TAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGRQN
                     TYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKMVVIGRQA"
     gene            706..>2132
                     /locus_tag="ACHKUN_004902"
     CDS             706..>2132
                     /locus_tag="ACHKUN_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6662992.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPG"
BASE COUNT          493 a          551 c          684 g          404 t
ORIGIN      
        1 aggcggtgta cgcccgtctt cacgaacctc tgggtgaggg ggcggtcatc catattgtgc
       61 cgcgactggc cggagccgga aagggcggac tgcagattgt gctgggggcg gcagccatcg
      121 tggggtcgtt cttcacggcc ggggcatcaa tggcgttatg gggttcagcc ctggcagccg
      181 gtggtttttc tgccaccacg atgctgtttt cactgggggc cagcatgata ctgggtggtg
      241 tggcacagat gcttgccccg aagccaaaaa caccggaata cagggcaacg gataacggca
      301 gacagaacac gtacttttcc tcgctggata acatgattgc ccaggggaac ccgatgccgg
      361 tgccttacgg tgaaatgctg gttggctccc ggcgaatctc ccaggacatc agtacccgtg
      421 atgaaggcgg tgacgggaag atggtggtta tcgggcggca ggcataaaag cgaaaaaatc
      481 ccgcagtgct cacggacagg aactgcggga gcgttacgaa gattgagtgt aaggaattat
      541 tcttatgtca cgacaaaaaa cattaacgca gagaaattat tagcgccaca gtcagtttgt
      601 gaaaatgtga agatattcag aatttttatt cagtcatgat acaggcatcc tccgggatgc
      661 ctgttgtttt tgtgcgtaac agttatcaca gtaaagggtg agacaatggg caaaggtggc
      721 ggcaaggcgc acacgccggt agaggcaaag gacaatctta agtccacgca gatgatgagc
      781 gtgattgatg ccattggtga agggccgatt gaaggcccgg tgaaggggct gcagagtatc
      841 ctggtgaaca aaaccccgct gacggacacg gacggcaatc ctgtgataca cggtgtgacc
      901 gcggtctggc gcgccgggga gcaggagcag acaccaccgg aaggctttga gtcctccgga
      961 gcggaaaccg gactgggcgt ggaagtgacg aaggcaaagc cggtaacgcg caccattaca
     1021 tccgcgaaca ttgaccgcct gcgggtcacc ttcggggtgc agtcactgtt ggagaccacc
     1081 tcaaagggcg accgtaatcc ctcttctgtc cgactgctga ttcagttgca gcgtaacggt
     1141 aactgggtga cggaaaagga tgtcaccatt aacggcaaga ccacctcgca gtttctggcg
     1201 tcggtgattc tggataatct gcctccccgc ccctttaaca tccggatggt cagggagacg
     1261 gcggacagca cctcggacca gctgcagaac agaacgctgt ggtcgtcata caccgaaatc
     1321 atcgatgtga aacagtgcta cccgaacacg gcgattgtgg ggctgcaggt ggatgcggag
     1381 cagttcggcg gtcagcagat gacggtgaac taccatatcc gcggtcgcat catccaggtg
     1441 ccgtcaaact acgacccgga aaaacgcact tacagcggta tctgggacgg cagcctgaaa
     1501 ccggcataca gcaacaaccc ggcctggtgc ctgtgggaca tgctgactca cccgcgctac
     1561 ggcatgggaa aacgtctggg ggcggcagac gtggacaaat gggcgctgta tgccattgcg
     1621 cagtactgcg accagacggt cccggatggc ttcgggggca cagagccgcg gatgaccttt
     1681 aatgcatacc tgtcacaaca gcgtaaggcg tgggacgttc tcagtgattt ctgctcggcg
     1741 atgcgctgta tgccggtatg gaacggccag acgctgacgt tcgttcagga ccgcccgtcg
     1801 gatgtggtgt ggccgtacac caacagcgat gtggtggtgg atgataacgg cgtggggttc
     1861 cgctacagct tcagtgccct gaaggaccgg cacacggcgg tggaggtgaa ttacaccgac
     1921 ccgcagaacg gctggcagac ctccacggaa ctggtggaag acccggaagc catactgcgc
     1981 tacgggcgca acctgctgaa gatggatgcg ttcggttgca ccagtcgcgg tcaggcccac
     2041 cgtgccgggc tgtgggtgat aaagaccgga ctgctggaaa cgcagacggt ggatttcacg
     2101 ctcgggtcac aggggctgcg tcacacaccc gg
//
LOCUS       DAZFRJ010000375         2123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000375 DAZFRJ010000000
VERSION     DAZFRJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.378"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..521
                     /locus_tag="ACHKUN_004903"
     CDS             <1..521
                     /locus_tag="ACHKUN_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6662993.1"
                     /translation="MIDPDPSVLKPDPSDKRSSCPDVSLPDEKQASPVERFLEKHPDA
                     HTWNVPKRQWGTREDLTCAQWIWGRVVALYEQAASDDGEVSRPREPNWTTWANDVRMM
                     RMLDGRSHRQICEMFGRVQRDSFWVKNIMSPAKLREKWDELVIRLGRSPAQRCVNHIS
                     EPDTEIPPGFRG"
     gene            528..1268
                     /locus_tag="ACHKUN_004904"
     CDS             528..1268
                     /locus_tag="ACHKUN_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6662994.1"
                     /translation="MKNITSGGVLARVSRFVPQDAIPPYRTVAEWREWQLAEGRKRSE
                     EVNRLNHQTRVEKIINRSGIQPLHRKCTFGNYRVQNDGQRHALSQAKSIAAELEGGCT
                     NFVFSGRPGTGKNHLAAAIGNHLLAKGRSVIVITVADVMLALHGSYDNKNSGEKFLQG
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASLLSVGMLTNLNHAAMNTLLGERV
                     MDRMSMNGGRWVTFNWESWRPNVSQHRN"
     gene            1298..2068
                     /locus_tag="ACHKUN_004905"
     CDS             1298..2068
                     /locus_tag="ACHKUN_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6662995.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCIPGADLPAEPEAASVTETDSKA
                     FPQPTGVALPVREAETQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
BASE COUNT          546 a          468 c          656 g          453 t
ORIGIN      
        1 cgatgattga ccccgatccg tcagtattaa aacctgatcc gtcagataaa agatcttctt
       61 gtccggacgt ttcactgccg gacgaaaaac aagcatcacc ggtcgagcga tttctggaga
      121 aacacccgga tgcgcatacc tggaatgtac caaagcgaca gtggggaacc cgggaggatt
      181 tgacgtgtgc acagtggatc tggggacggg ttgttgcgtt gtatgaacag gccgccagtg
      241 atgatgggga ggtatcacgc cccagagagc ctaactggac gacctgggcg aatgatgtgc
      301 gcatgatgcg tatgctggat ggacgtagtc accgacagat ttgtgaaatg tttgggcgtg
      361 ttcaacggga ttcgttctgg gtaaaaaaca tcatgagtcc ggcaaaactc cgggaaaaat
      421 gggatgaact ggttatccgc ctggggcgtt cgcccgcgca gcgttgcgtg aatcatattt
      481 ctgaaccgga taccgaaatt ccgccgggtt tcagggggta gcgcaccatg aaaaacatta
      541 cgtcaggtgg tgttctggca agagtcagca gatttgtgcc gcaggatgca atccctccgt
      601 accgtacggt ggcggagtgg cgggaatggc agcttgctga agggcgtaag cgaagcgagg
      661 aggttaatcg tctgaatcat cagacgcggg ttgaaaaaat cattaaccgc tccggtatcc
      721 agccgcttca ccggaagtgt acgttcggta actaccgggt gcagaacgat ggtcagcgcc
      781 atgccctgag tcaggcgaaa tccattgcgg cagagctgga aggcggctgt acgaattttg
      841 ttttcagtgg caggcctggc acaggaaaaa accacctggc ggcggctatt ggcaaccacc
      901 ttctggcgaa aggtcgcagt gtgattgtga taacagtggc ggatgtgatg ctggcgttac
      961 atggcagcta cgacaacaaa aactcgggcg aaaaattttt gcagggattg tgtggcgttg
     1021 acctgctggt actggatgaa attggcatgc aacgggatac gcgtaatgag caggtcacgc
     1081 tgaatcagat tgttgaccgc agaacggcat cgttactcag tgtggggatg ctgacaaatc
     1141 ttaaccatgc agcgatgaat acacttctcg gcgagcgggt gatggaccgc atgtccatga
     1201 acggtggtcg ctgggtgacg tttaactggg agagctggcg tccgaacgtc agccagcaca
     1261 ggaactgaga agtaattttt atccggagga agttttaatg gaaaccgtat tgcatgcact
     1321 gaaagcgatg ggtaaagcca attctgttga actggcggcg cggcttgata tcagccgtga
     1381 agaagttctc aacgaactgt gggaactcaa aaaaaatggc gttgttgata aaacgggtca
     1441 cacctggttt ctggctggcg aaggtgaatc ccgggtaacc gaagagcggc cagtaaaatc
     1501 tgaagcacag gatatgctga ccggggaggt cgaacaaaaa gttaccgcag acatgatgat
     1561 tgagtttatc ggtcaggatg gtgctaaaac gtgtgaggaa ctggcgggta agttcggcgt
     1621 cagtactcgc aaggttgcct ccacgctggc ggtggtaacc gcaacggggc ggctggcacg
     1681 cgttaatcag aacggtaaat ttcgttactg cataccgggc gctgatttac cggcagagcc
     1741 ggaagctgca tcggtaacgg aaaccgatag taaagccttt cctcagccaa caggtgttgc
     1801 gttaccagtc cgggaagcgg aaacacagga agaaataaaa acggaaagtg tggcggtcac
     1861 agtgcagtca cagccgtcgt tcaccagaaa gcatccggat ggtctgattt taccatcgct
     1921 gcatgtggct aaccgcgagc tgcgccgggc aaaaggtcag gttcagaagt gggagcgtgt
     1981 ctgcgccgcg ctgcgggagc tgaacaagca ccgggatatt gttcgacaga ttactgattc
     2041 ttcccgccgt gttgtatcgg aaaagtgatt gccggaggcg cttatggcaa aagtatttac
     2101 accagaagat cgggaagaag tga
//
LOCUS       DAZFRJ010000376         2090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000376 DAZFRJ010000000
VERSION     DAZFRJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.379"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..188)
                     /locus_tag="ACHKUN_004906"
     CDS             complement(<1..188)
                     /locus_tag="ACHKUN_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6662996.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWV"
     gene            complement(172..453)
                     /locus_tag="ACHKUN_004907"
     CDS             complement(172..453)
                     /locus_tag="ACHKUN_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO6662997.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            554..973
                     /locus_tag="ACHKUN_004908"
     CDS             554..973
                     /locus_tag="ACHKUN_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6662998.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADKLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            1239..1391
                     /locus_tag="ACHKUN_004909"
     CDS             1239..1391
                     /locus_tag="ACHKUN_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6662999.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LARNTD"
     gene            1403..2041
                     /locus_tag="ACHKUN_004910"
     CDS             1403..2041
                     /locus_tag="ACHKUN_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663000.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLDKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
BASE COUNT          656 a          450 c          461 g          523 t
ORIGIN      
        1 tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg gctgtcgtca
       61 tacagttctg ggaacgtcat acccaactca aaataagcct gggttattcc agctgctgga
      121 actttttcgc catcaggacg cgcccaggca ttcatcgcca tgcggatgtg ttcatgcttg
      181 attttcatga atcaagctcc tagaaagtgg ttgtgttaac gttttggtat cttccagctc
      241 gggccaaata ttcatccaat caaaaggcct tagttgctga cgtgtaactt caccattact
      301 ggctcgctca ataaggacac ataacgatgc ccctaacact tgacctttac tcaatgcctt
      361 tcttagataa ccgatgctgg taccacactc gcatgcaaac atacgctgtt catctgacga
      421 aagagaattg agaaatattc ttaattcttc catagctact ccttagtaaa cacagcaaag
      481 aatacccaca ggtaaacaaa agtcaatacc cacaggttgt ttaccttgcg gtaatcgcat
      541 ctattattta cctatggaca aatatgaatt tagacgacag caactcatca aaattcgtga
      601 tgagaaatgc gatggtaaag cggttaacgt ggccagaaag atcgggcgcg agccttctta
      661 tgtatcaaga atgttgtacc cagaggggaa aaagggaaaa aaacggatcg ctgatgatat
      721 ggtggagatt atcgaagagt cctttgggtt accccgggga tggatggatg gtatcgtttc
      781 atcatcaacg aacacagcct ccagttatga aacaagggtt ctaacgccac gacaacgtat
      841 ttttttagat ctcttagacg aactgccaga aagtgaagcg gataaattat taaaaactct
      901 tgaagagaaa aaacagtatt acaatatgat ctacgaagaa atccgtaaaa agaaagcaca
      961 aaacgcatca tagctcacca aacaactagt caccagttaa gacaccgcaa aaatttaccc
     1021 atgggtattt actttttaaa tacctatggg tatccttctt ttcataccaa cccaccccgc
     1081 cccacagaat gcagggcaat acttcgagtt accaggcagt ggtcaggggt taagtagcca
     1141 gcccgaggcg taagaacatg acggcagggt tcaactttaa taactatgca gcaggttttt
     1201 gttccgctac cccggcgtta aggggaaatg aggtcagcat ggatactatc gatcttggca
     1261 acaacgaatc tctggtatgt ggcgtgtttc ccaatcagga cggcacgttt accgcgatga
     1321 cgtataccag aagcaaaacg tttaaaactg aagctggcgc gcgtcgctgg ttagccagaa
     1381 acactgactg atgaggttga cgatggaatt taaagattta cctccttcaa tccaggagat
     1441 tgcagcacac acacttcgtc atcgtctgaa cgaacttgaa ttggaatcgg taacgaaaaa
     1501 agacactgat aatatggctc gtaatgtgcg cgatgcgttt accggattgt atttctgtgc
     1561 gtctataaat aaacacgact cagagagtgt ggcaaataaa attgcagaaa cgacagcgca
     1621 aaacatcaat acgaaaccaa cggaagaaga aattgatcag tttgctcatg atgctggttt
     1681 aaaaaacaag aaagaaaaat cgccatatgc ggggaacatg tttgtttatg acaatctcat
     1741 cagaattcgt ggcgaaattc cggcggaata cctggcaaga gtccatcagg cattgcttaa
     1801 aaatttggaa acagaattat ttgatggcaa cactaacggt ttcttcatgg tatcaggcct
     1861 tgataaagac tgggatgcag aaaaacgctg gaatgttgct acatggttat tcagtaacag
     1921 agccgctgca ctggaagctt cggcatgtat ttgcggcctg ttcttaacag accacaaata
     1981 taatctggat gtgtacagtt atatttacgc tgaacacggt ccactctgga ttgactggta
     2041 attataagga aacaccagca gggccgcggc gaccaacaga acgattaaaa
//
LOCUS       DAZFRJ010000377         2081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000377 DAZFRJ010000000
VERSION     DAZFRJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.380"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..276
                     /locus_tag="ACHKUN_004911"
     CDS             <1..276
                     /locus_tag="ACHKUN_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6663001.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            328..711
                     /gene="gpFI"
                     /locus_tag="ACHKUN_004912"
     CDS             328..711
                     /gene="gpFI"
                     /locus_tag="ACHKUN_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6663002.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            704..1057
                     /locus_tag="ACHKUN_004913"
     CDS             704..1057
                     /locus_tag="ACHKUN_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6663003.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1072..1605
                     /locus_tag="ACHKUN_004914"
     CDS             1072..1605
                     /locus_tag="ACHKUN_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663004.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1602..1997
                     /locus_tag="ACHKUN_004915"
     CDS             1602..1997
                     /locus_tag="ACHKUN_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6663005.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2005..>2081
                     /locus_tag="ACHKUN_004916"
     CDS             2005..>2081
                     /locus_tag="ACHKUN_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663006.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDA"
BASE COUNT          461 a          483 c          666 g          471 t
ORIGIN      
        1 gtctggcgtc tgctgaatgg ttttaagctg ttccgcgaaa aactggatac ccgtcgcggc
       61 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc tttcaaaggg
      121 tattacggcg atctggccat tgtggtggcc tcttcccgtt atccgaaaca ctggctgacg
      181 gtaggggatc ccgcccgtga atttaccatg acgcagtccg cgccgctgat ggtgttgccg
      241 gacccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcggggg cgaaatgccc
      301 ccgtgtcttt tttcacaggg ggctgatatg gcaacaaaag aagaaaatca gaaacatctt
      361 cgtcaactgg ctggcctgct ggggcgcgag gcggatatgt cggggagtgc tgcggatatt
      421 gcgcaacgtg tgtctgagtg ggaagaggag cttgctgctt cccgggaggg cattatgcct
      481 ggtgatgaga gcgggcctga gcaaaatcac acagacgatg gtgagcagtt gcacaacact
      541 gatgctacgg atgatgttaa agcggtccgt gtgcggaaat gcctgcatgt gatggggtat
      601 tgcccggaga caggccgtcc cgttgaactg acgtaccggg gcatgcgtgt tctggtgcca
      661 tcaccactgg cgacagccat gatacagcac ggaacggctg agcatgcgtg attttcagaa
      721 tgcctttgat gctgcccttg ccggggtgga cagcacgatt gttgaagtga tggggctctg
      781 tgcgcagttc acctcggggg cacagtgtgg cagcgaagtt cagggggttt ttgacgatcc
      841 ggagtcgctg gggtttgccg gtagcggggt ccgtattgaa ggaagctgcc cgtcattatt
      901 tgtacggacg gatacggttc gtgctgtgcg gcgtggtgac acgctgacca ttaatggtga
      961 gacattctgg gtggatcgtg tttctccgga tgacgggggc agttgttatc tctggctcaa
     1021 ccgtgggcaa ccaccggcag ttaaccggcg acgataaacg cagggtgaat tatggcgata
     1081 aaagggcttg atcaggcgat tgaaaatctg agccgggttc gtaaaaacgc cattccggcg
     1141 gcttcagcaa tggccattaa ccgcgtggcc acaacggcga ttaatcagtc ttcgtcacag
     1201 gttgcccggg agaccagggt gagccggaaa ctggtaaagg aacggtccag actgaaacgg
     1261 gccacggtca gaaatccgaa tgccagaatt atcgttaacc gcggtgatct cccggtgatt
     1321 aagctgggga tcaggatgcc ggggcgtcgt ccggacagca tactcaaagc cggtcagcat
     1381 cggtatcagc gggcatttat tcagcgatta aaaaatggtc gctggcatgt catgcagcgt
     1441 gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggccccactg
     1501 aaacaggcgt ttgatgagaa tgttgaccgt atccggcgtg aacgcctgcc cggagaactg
     1561 gcatatgcgc tgaaacaaca actgaggatt gcgataaaac gatgaaacat actgatatcc
     1621 gtgctgcagt gctggatgca ctggagctgc atgaacacgg ggcgacgctg tttgatggtc
     1681 gccccgttgt ttttgacgaa gaggattttc ccgcggtcgc ggtttatctg acggatgcag
     1741 agtataccgg tgaagagctg gatgcggata cctggcgggc cacactgcat attgaagttt
     1801 tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc cggatttatc
     1861 cggcgatgtc cgcgatccct gcactggcag ggatgattac cacgatggtt cagcagggct
     1921 atgactatcg tcgtgatgac gatatggcgt tatggagctc tgcagatttg acttattcca
     1981 ttacatacga gatgtgagga cgatatgcca acaccaaatc ccctggcgcc ggtaaaaggt
     2041 gccggtacca ctctgtgggt ttacaccggc aagggtgatg c
//
LOCUS       DAZFRJ010000378         2043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000378 DAZFRJ010000000
VERSION     DAZFRJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.381"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..512)
                     /locus_tag="ACHKUN_004917"
                     /pseudo
     CDS             complement(1..512)
                     /locus_tag="ACHKUN_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(472..591)
                     /locus_tag="ACHKUN_004918"
                     /pseudo
     CDS             complement(472..591)
                     /locus_tag="ACHKUN_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(604..>2043)
                     /locus_tag="ACHKUN_004919"
     CDS             complement(604..>2043)
                     /locus_tag="ACHKUN_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663007.1"
                     /translation="YRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWG
                     TFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTP
                     DQIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALG
                     LYLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNT
                     SSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERIT
                     LQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYG
                     IKRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYK
                     ESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPS
                     PIIPLNWSNGSMTLMLKEMSNLINDEGIK"
BASE COUNT          526 a          452 c          369 g          696 t
ORIGIN      
        1 caattacaaa atgaatggac caatataatg attttttgtc taacactgga gcgtttccgt
       61 attggcagac tgtgacttta tctaaccaaa gattcaattc ccctgttcat attcttatta
      121 gtggttgagc agggctcaat actccatcaa atactttacc tacgtgcaaa cgattatatt
      181 ttagttcagg atgctttaga tttaacgggg aactggataa actgagccat tcccctatta
      241 acctttccgt ttctggcatt aaagccatcc cagcgagctc agatgaaatt aagggaaata
      301 tttctccaag caatacccat ccagaccagc tgccactctc ttctgctatc ggtaaccatt
      361 tagcactaaa ttcgggcatt aataatgcaa catcttcgcc attttctttc caattttgaa
      421 aagtggtttc aaacgtatga gtgtttcgat ttactttcct cagaccgaac attaactttc
      481 atccttctga tcgttttcga atttgctttc attttcttgc tcctcataag gctgatgtcg
      541 ttgtccttgt cgttcatctt gctcagtcaa tacccaacgt tgtgctgtaa ctcattatga
      601 aaactattta attccctcat cattgataag gttggacatc tctttcaaca tgagagtcat
      661 tgaaccattc gaccaattta gcggaatgat agggcttggc gcttcatgca aagttcccga
      721 cttgtggata tagttatagc tttcgggaag tgccatttca ggtacactcc tgccatttgc
      781 accaatcata ttctcgcctg ttatttgggc caatgatcta ttcataaatt gcactgagtc
      841 attcaaatac tcttcctttc ttgattcttt ataaacaatt gctgctgatt ttgcatacca
      901 tgagtcaaaa aaccattctg cttctgttga tggaatgaag ctcttttctc ttttcgcatg
      961 tgaattttga tcggtatcgg ttttaatatc attgaaccaa aaatttgccg actgataatt
     1021 atctttctcg tatcttttta taccatacgg acctgctagt tttttaacta tttttagaac
     1081 ctgctctttt cttctggtgt ttatctttgc aagatttgca ggataaataa cattaagtaa
     1141 agccgcatcg gcttctctgt aatgtttatc tttttccaga taacctgggg attctccccc
     1201 taaatcaagc tgtaacgtta ttctttcata acctttatct atgaggtggt tcaacctcgt
     1261 tgttgaaaga ggttcatcca gttcattggc tttagcttcg cgtagtaaat cagagacaaa
     1321 aacagagtct ttcttcgaga gtaagttcga caaacgttcc agtccagagg tcaccagtgc
     1381 aacggaagat gtgtttaatc gggcatcttc ttcccatgct ccggaatctt ccataacata
     1441 gaaattggct ttatccagat aagcaatcag cagcgcaacc gattttaatc ggtcaccttt
     1501 ttgccaatct tcagcgttaa ttgtatcagt gtcgattgct tgtataagga gatcaagata
     1561 aagacctaac gcatcatttt gtttatggtt ccacagctgc ggcttgcctt cttcctgcac
     1621 atctgccatc acgggggagt tgctgtcaaa tcggatatgc acaacattca tttgcccggg
     1681 aactccatcc agtcgtttgg ggttgctaat gacgtcctgc atccgcttaa tctggtcggg
     1741 cgtagacata taatcccaca gggtgagcag aacctttttt gcggctacac tatttccctg
     1801 atctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
     1861 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
     1921 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
     1981 gtctttggta tagtggctct ttatttcatt ttgtaatttg gctgagtgaa tcaaaagacg
     2041 ata
//
LOCUS       DAZFRJ010000379         2025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000379 DAZFRJ010000000
VERSION     DAZFRJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.382"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1336)
                     /locus_tag="ACHKUN_004920"
     CDS             complement(<1..1336)
                     /locus_tag="ACHKUN_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO6663008.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDD"
     gene            complement(1333..>2025)
                     /locus_tag="ACHKUN_004921"
     CDS             complement(1333..>2025)
                     /locus_tag="ACHKUN_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6663009.1"
                     /translation="QKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDA
                     RDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAE
                     CCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAGY
                     TFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLAYYS
                     ACPETLKDMSDL"
BASE COUNT          551 a          494 c          464 g          516 t
ORIGIN      
        1 catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa
       61 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatctt
      121 ctgcatcaag gcggctcaca atcggcatta atgattcttc ggttggttgg ttcgcctgca
      181 ctaattgttt ggcaactaaa tataatggcc ataagggatg ttctgtatca cgcgtgagtg
      241 agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagagcag
      301 agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg
      361 ggatataaag tagcggtttt ccctgatcgt taaccagttc acgagcttca atcatcatca
      421 ccactaacgg gtaataatcg acccgatgct gacggcacca ttgcaatatt aaagcatccg
      481 cctgggtgga gggatactcg cgccagtagt cacaaagttc tgccagcaag ctttcgcctt
      541 ctccacaata gacgcgttgt gcgtattcat aataacgcgc atcttcatta tcagattcag
      601 cttcccgcca cgaccgggcg taatccagga gttcgggaat tcccaggcga cctgcgctat
      661 accagcggag taaatttctg tgcagttttg catcatcggg gataatcact gcgcgatgtt
      721 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg aacatttggt
      781 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc tggaagttaa
      841 aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
      901 cgacgttttc ctcttgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
      961 ataaatgggc agtgttcatg gcgattgtgc acagcgacca gcgtaattca tcaatctctt
     1021 ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
     1081 cgctatcaga ggcaagtaac gcacgaaagg cgagcagaat ttcatgttct gctacctcat
     1141 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccgtagc gcctcctcat
     1201 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
     1261 gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctatt tcgagaatct
     1321 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg cagaatagta
     1381 agccaatggt tgccaggaca aggtagggta atacgcatca tcactggcaa ataattgata
     1441 aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcgc gtaataactg
     1501 cagatgatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
     1561 ttgccgccag ttacgataat aacgtagagc ccgtagatga atgcgcgact ggatccatag
     1621 actttcttct tcgctcaacc agttattaag tacgcccatt cggcttaaaa aacccatccg
     1681 gacataatcc caggctttaa ttcctccctc accacagcat tcggcggtgc tggcgacaaa
     1741 ttgcgcataa gcctgacctt gctcatttag ttcagcaaga tagtcacgcc attcacatgg
     1801 cgaataacga aaccagcggt gataaaaccc tgccaaccga gcggcatgac cgttatcgat
     1861 cattcggtga attgtgcgca gcaggtcatc acgggcatca attccccagg aggaagaaag
     1921 gtcaaaaaag catgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc
     1981 attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttg
//
LOCUS       DAZFRJ010000380         2004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000380 DAZFRJ010000000
VERSION     DAZFRJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.383"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(348..782)
                     /gene="uspF"
                     /locus_tag="ACHKUN_004922"
     CDS             complement(348..782)
                     /gene="uspF"
                     /locus_tag="ACHKUN_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO6663010.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(923..>2004)
                     /gene="ompN"
                     /locus_tag="ACHKUN_004923"
     CDS             complement(923..>2004)
                     /gene="ompN"
                     /locus_tag="ACHKUN_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO6663011.1"
                     /translation="AHAAEVYNKDGNKLDLYGKVDGLHYFSDNSAKDGDQSYARLGFK
                     GETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGLKFADYGSFDYGRNYGVMY
                     DIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFFGLVNGLNFAVQYQGNNEG
                     ASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAYTSSDRTNDQVNHTAAGGD
                     KADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVANKTQNFEVTAQYQFDFGLR
                     PAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATYYFNKNMSTYVDYKINLLD
                     EDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          546 a          442 c          459 g          557 t
ORIGIN      
        1 gggcaaaaat ggggcaaaaa aagtgtagat tattgtgctt tattgtagtt gttaatcctg
       61 cctaacatat tgaaaaacat aaaagttatt gttcgtcaga tagttgcggt ttttactctg
      121 acttgttcag gttgtattgt tctttcttac taatttcttg attttgcgac atttaaaagc
      181 gactcaattc gttatatggc atcagaagag tatgcgtcat gccggaacgc ccagcataag
      241 aaatctgata taaaaactgt ggcgtgtatg gtacggatta gaggggaaaa tgtcagcaca
      301 tttgcgaaat gaatcaaaaa gcccgcagca atgtgcgggc gttagtgtca gcgcacaacc
      361 agcacggagc actctgcgtg acgcactaca gctgcggcgt tagaaccgag cagataagtg
      421 gtgatatccg gtcgatggga agcaatgatg atcatatgag cggggatctt cttcgccaat
      481 tccagaatgc ggtcttttgg cgagccttcc tcaacatgga catgcactct gtcggttggc
      541 agtttaaatt ttttaatgat ctcttccagt tgcgatttgg cttccgcttt caggtcatcc
      601 attgccggta attctgcgga atacgctaaa cccagagagg catagtaggg cagtgaaggt
      661 attaccgtca ggaaatgaac ctctgcatca tcaatctttg cctctgcctc aacgtggcta
      721 atcacgcgtt gagttaattc tgaatcggaa atatcgatag ggacaagaat cgttctgttc
      781 ataaaacctc ctgttttagt atccgcataa agtgtaacgc cagatgacac tttttgtgta
      841 atgacggagt tcacattttt aatttagatc aaaggaggaa gaataagcag aaaaagcccg
      901 ccataacagc gggcaggagg atttagaact gataaaccag acctaaagcg acaatatcat
      961 cggtagagat gccgttggca gcgtagaaac tgtcatcttc atccaacagg ttgattttat
     1021 agtcaacata agtggacatg tttttattga aatagtaagt cgcgccaaca tcggcgtatt
     1081 taaccagatc tttatcatca acacctgccg ggttgtctgc accaccggca gcgtgcaggt
     1141 cacggccttt agacatcagg aaagagactg ccggacgcag accaaaatca aactggtact
     1201 gtgcagtgac ttcaaagttc tgggttttgt ttgcaacagc ataatcgctg tcgccaaacg
     1261 gggtcatatt acgcgtttct gaatacatgg ttgccaggta aatattgtta gcatcgtatt
     1321 ttagcccagc agtccacgcg tctgctttat caccacccgc cgcagtatgg ttaacctggt
     1381 cattggtgcg gtcagaagag gtgtatgccg caccagcgct aaagcccatg cctaaatcat
     1441 atgttgtgga aagaccccag ccgtcaccgt tttcatggcg aacatcacgt ccgttgttgg
     1501 tgccttcctg accattactg gcaccttcgt tgttaccttg atactgcacc gcgaagttca
     1561 gaccatttac cagaccgaag aaatcagtat tacgataagt cgcgacgcca ttggctcgac
     1621 cagtcataaa gttgtctgca ttggtataag agtcaccgcc aaattcaggc agcatatcgg
     1681 tccagccttc gatgtcgtac attacgccat aattacgtcc gtaatcgaaa gaaccgtaat
     1741 ctgcaaattt cagtccggca aatgccagac gggtccatga ctggtttttt gaagattcag
     1801 tgttgtttgc ctgaatattg tattcccatt gaccgtagcc agtaagttga tcgttaattt
     1861 gggtttcgcc tttaaaaccc agacgcgcat agctctggtc gccatctttc gctgaattat
     1921 cagaaaaata atgcaggcca tcaactttgc catacagatc taatttgttg ccgtctttat
     1981 tataaacttc ggctgcatgt gcag
//
LOCUS       DAZFRJ010000381         1998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000381 DAZFRJ010000000
VERSION     DAZFRJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.384"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..304
                     /locus_tag="ACHKUN_004924"
     CDS             <1..304
                     /locus_tag="ACHKUN_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663012.1"
                     /translation="FIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEE
                     IVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
     gene            complement(308..397)
                     /gene="yldA"
                     /locus_tag="ACHKUN_004925"
     CDS             complement(308..397)
                     /gene="yldA"
                     /locus_tag="ACHKUN_004925"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO6663013.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(535..945)
                     /gene="rnk"
                     /locus_tag="ACHKUN_004926"
     CDS             complement(535..945)
                     /gene="rnk"
                     /locus_tag="ACHKUN_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO6663014.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(1175..1981)
                     /gene="rna"
                     /locus_tag="ACHKUN_004927"
     CDS             complement(1175..1981)
                     /gene="rna"
                     /locus_tag="ACHKUN_004927"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO6663015.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
BASE COUNT          502 a          462 c          532 g          502 t
ORIGIN      
        1 atttattcac ggtttcctgt ttggcgacgc ctttgataaa gggttgacgt ttaaaatggg
       61 acagacccac gttcacgcat ggctgggaga attattaccg ttaattgaga aaggattact
      121 gaaaccagaa gaaattgtta cccactatat gccgtttgaa gaggccgccc ggggatatga
      181 gattttcgaa aaacgtgaag aggagtgccg taaggtgatt ctggtacccg gtgcacaaag
      241 cgcagaggcg gcgcagaagg cggtttcagg tctggtgaat gcgatgccgg ggggaacaat
      301 atgatcgtca ggagtggttt tcgaggtaaa ggacagccat gacgataatc gccgccataa
      361 tcagaaatcc tatcaggatg taaaatgctt ctgccatggt tattcccaca aacgaaacgg
      421 aataattttg cagcaaaagt gaacagtgag aaccaggaaa aattgctgat tttgcgtaaa
      481 gaggatgcga gtgcatcctc tgggcaaagc gagttatcgc ttgtgcagat gggattaaag
      541 caggtagtcg ccagcagctt ctggctggta ttcgagttcc agcacttcaa ggtgggttgc
      601 aacgccgccc ggaagttccc agtgaatgga atcgccaacg cgcagcccca gcagtgcggc
      661 acctaccgga gccataacgg aaagctgagt attgctatcg gtcatttttg ccggatacac
      721 cagcgtgcgc acacgcactt cgccatcgct aagattgcgg aatttaaccc ggctgttcat
      781 tgtcaccacg tcgtgtggca tctcttctgg cgaacacatt tgggcgcgat ccaactctgc
      841 gtttaacgcg tcggcgattg gcaaaccagc ataggcgggt tgctccagca gaatatcgat
      901 gcgttcggca tccaggtcgt taatgatgat agttggtctg gacattttta ctccatgtcg
      961 tcggtactgc gagtgtcgca gataaacata cccaaaagaa aaccctcacc gtcaggcggc
     1021 gagggtttaa ctcacatgat gatactgact gttgctcact ctttgaagtg atttgcgtca
     1081 cattcaggga attcctcaat gcagcgcatt atgtataatt cttaatcgtc acggtttatg
     1141 gaagacgaat tagcgtgttt tgtaaatcag atgattaata acccacttta tcaatcacaa
     1201 aggttttgcc acagttgcct gggtgtggtt gtggcaagaa tgagtttgca gaaagcggag
     1261 cgttgattgc gtcagctttg atcgagatct gaatttcagt caaatacgca gggttaccct
     1321 ggcacgttag cttaactgct ttcacgttct cttttcccca gcttttggca aaggcggcgt
     1381 caaagtcacg gcggctcact gttttaccgt aattatccgc aaggaatttt ccggcttcgc
     1441 tctctttaat ttcttgattc aggcgtacca tcgtaccgaa gtatgcgtcc ggatcaaaac
     1501 caaagcaggc accgtgtttg gcatattcgt agcgttccag gcaggaacgt ccgccagctc
     1561 ctggcatgac ttcacttagt ttagcggccg tttccagtga taatccggtt tccggcgatg
     1621 aacacatccg gctggcgtgc gcttctggta gattcgggat tgggcgagta gcgcaaccga
     1681 agcgcatcca gcggcgttca tcaacaccac gggcagcaac cgatttaggc aatcctggcc
     1741 acagaccatg tacggtcaga aaatcagctt tgttggtcgt ttcggtttgc aggcgacatt
     1801 catctcgttc gttacgattt cgatcgtgtt gactctggca aaatccggtt tgccaggaga
     1861 gggccaggac atagcgatca aaatcgccat actgttttgc ctgcaacgct aaggcgttgg
     1921 cagaagagaa gggaagcaga gaaaccgcga gcaacgcggc gttacgccag aatgctttca
     1981 taatgggtgt ggaactca
//
LOCUS       DAZFRJ010000382         1989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000382 DAZFRJ010000000
VERSION     DAZFRJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.385"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1830)
                     /locus_tag="ACHKUN_004928"
     CDS             complement(<1..1830)
                     /locus_tag="ACHKUN_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6663016.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVLWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGVTALLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAAN
                     QYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGT
                     FGAFDNEWHTLAFRFAGNNSLQVTPVIDGQVGTPFTLTQSPVSA"
     gene            1807..1950
                     /locus_tag="ACHKUN_004929"
                     /pseudo
     CDS             1807..1950
                     /locus_tag="ACHKUN_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          430 a          612 c          485 g          462 t
ORIGIN      
        1 ggcactgacc ggtgactgcg tcagcgtgaa cggtgtgcca acctgaccat caataaccgg
       61 cgtcacctga aggctgttat tcccggcaaa gcggaaagcc agcgtatgcc attcgttatc
      121 aaatgcgcca aaggttccca gtttcaggtt gtttgtcgcc actttcgcat tgtggtacat
      181 cacattcagg tcttttgcat ctgtctggat gtagaacgct gccagcaggt tattcccccc
      241 gtctccggtc agggcaacgc cctgtggcag tgaagatacc ggccagtaaa acgccataac
      301 atactggttc gcagccagcg ctcccgaaac cttaaagcgg cagcgaatct gccccccttt
      361 ctgtaacaga gccgcaccgt tgcccgcggc gtactccagc acccagctgc ttttaccggc
      421 ttccttggtc agcttcactg ccttacctcc ggttccctcc gcatcgctga ccacttctgc
      481 cctgccgcca ctggctgacc atccctgtac tttcaggctt ccctctgact cgctggcaag
      541 gtaagagagc agtgctgtga cgcctgtggc ttctgcaccg gaaggcgatg acgggcgcac
      601 ctctgatact gtcgatgatg cccccgcgtt tagcgccact cttcccgcat ggcgcaaaat
      661 cgccgttgcc agacggtcgg aaataatccc gcggcgagcc catgaactga aatggctcgc
      721 cctgtcctgt gacgtccagg tggctgagct gtcacgccat ttcgaaccgt aatatccgat
      781 acccggaatg tccgggtctt cttccggttt gttcgtcggc acattcaccc cgttctcatc
      841 cgtcatgaac ggtacgaaat ggatattctt ttccgttttg tttttgtagc tgccgtacac
      901 cgtctggtac gaggattcgt tcttctgctt ccagaaatac gtcgtatctc cacatatcca
      961 gggaacaccg ccagcagagc caccgacgca ctgacctgcc atatccgcca ggtctgcacg
     1021 gaatttatca accagcgcac caaactgtgc tgcgtgattt accggcgtac cgccaaaatc
     1081 aaattccccc tgcatccaca acacggcaaa cagcacattt ttcgggttct tcttcagtgc
     1141 tgcttttgtt cgaccgataa ggtccttata cagcggcttg tccacacccc agcgggttga
     1201 attctccgag gcaccactcg cgtcactgta tgtgccatcg gctccggtgg tgaacgctga
     1261 accaccacga cagcacggaa ccagcagaat gcccgcattc gccggtataa acggcagcaa
     1321 ttttttggcg atatgcagcc cttgccccac ggttccgtac tgcccctttg acaggtccgc
     1381 tttcggatgg ttaaggcggc tcatgtcctg cacatcatgc agacaatggt ccgccggaat
     1441 gatgtcgtta tatttgcatg ctgcaccgcc cggtgtcacc gtactgcggc gcgccagctg
     1501 cttaatacgc gggtccggac ggtcatatgt ctccggcagc ggaaggcctt caccatatgc
     1561 catgctgttt gactgccccg ccagaaccac aacaaagtaa tactccgtgt ctctggtggc
     1621 gctgattact gtgccttctc catccgacgg cttcaccaac acaggtgtgg tgacatcacc
     1681 ttccgccgca atggcctgca tcagagtata aggcgtgatg gccaccggac taccaaacgg
     1741 ctgccagccc tctttcagtt tgtgtgtcag cttttccgca aggtctgacg gcgacgccgc
     1801 cctgacaaca tcataatgtt taatcgacat cgaatttctc ccgtgtacag gaacagagtt
     1861 aaaaagccgg aaccggaatc aaattacagg atggccatct gccagtggct ggtcgtaaaa
     1921 aaaaggccac gccatgcgca gccggaaata aagggataac gatgatagtt tgagaaaaac
     1981 agaaacaac
//
LOCUS       DAZFRJ010000383         1973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000383 DAZFRJ010000000
VERSION     DAZFRJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.386"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..1739
                     /gene="yfbK"
                     /locus_tag="ACHKUN_004930"
     CDS             3..1739
                     /gene="yfbK"
                     /locus_tag="ACHKUN_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO6663017.1"
                     /translation="MMLLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAAQQAAIK
                     EAEQRSVADKATADTKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIAN
                     PGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIV
                     NYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPAS
                     NLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSH
                     KAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPK
                     SIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSE
                     MRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHIT
                     LLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGP
                     TINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRL
                     IELADGVTDISQ"
     gene            complement(1810..>1973)
                     /locus_tag="ACHKUN_004931"
     CDS             complement(1810..>1973)
                     /locus_tag="ACHKUN_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO6663018.1"
                     /translation="SAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIKHSQPK
                     NRASGKRVS"
BASE COUNT          571 a          430 c          486 g          486 t
ORIGIN      
        1 taatcatgtt gcttatgagt agtttgattt tgtcaggatg cgggccggaa tctgagaata
       61 aggaaagtca gcaacaacag cccagtaccc ccacagacca gcaagtgctt gccgcgcaac
      121 aagctgcaat aaaagaggcc gagcaaagat ccgtcgccga taaagccact gcagacacga
      181 aagcaaaagc cttagcccag caagaagcgc aacaatattc agacaaacag gctttgcagg
      241 ggcgcttaca ggcggctcca aaatatcaac atgcagccag agaaaaagca gcgtcccaaa
      301 tcgcaaatcc aggaaccgct cgctacaagc agttcgatga taatccggtt aagcaggtag
      361 cgcaaaaccc gttggcgacg tttagccttg acgtcgacac cggcagttat gcgaatgtac
      421 gccgttttct caatcatggg ctgttacctc cgccagacgc tgtgcgggtg gaggagatag
      481 tcaattattt cccgtatgac tgggatatca aagacaaaca atctattccg gccactaagc
      541 caataccttt cgctatgcgc tacgaactgg ctcctgcgcc gtggaatgaa cagctaacat
      601 tgctgaaaat tgatatcctg gcgaaagatc ataaaagtga agagttacca gcttctaatc
      661 tggtctttct tatcgacact tctggttcaa tgatttctga tgaacgtttg ccacttatcc
      721 agtcttcgtt gaaattattg gtcaaagaac ttcgtgagca ggataacatt gccatcgtga
      781 cctacgctgg cgactcccgt attgcgttgc cttctatctc cggtagtcac aaggcggaaa
      841 ttaatgccgc aattgattcg ctggatgccg aaggcagtac caatggcggt gccgggctgg
      901 aactggctta tcagcaggcg gcgaaggggt ttattaaggg cggcatcaat cgcattttat
      961 tagccactga cggtgacttt aacgttggca ttgacgatcc aaaatcgatt gaatcaatgg
     1021 tcaaaaaaca gcgggagtct ggtgttactc tgtcgacgtt tggcgtgggg aatagcaatt
     1081 acaacgaggc aatgatggtg cgaattgccg atgttggtaa cggcaactac agctacattg
     1141 ataccctctc tgaagcgcag aaagtattga atagtgaaat gcggcagacg ttgattaccg
     1201 tagcaaaaga tgtcaaagcg caaattgagt ttaaccccgc gtgggtaacg gaataccgtc
     1261 agattggtta tgaaaagcgc caacttcggg cggaagattt taataacgac aacgttgatg
     1321 cgggggatat tggcgcaggc aaacatataa cgttgttatt cgaattaacg ctgaacgggc
     1381 aaaaagcatc aattgataag ttacgctatg ccccggataa caaatcagtg aaatcggaca
     1441 aaacgaaaga actggcctgg ttaaagattc gctggaaata cccgcaggga aaagaaagtc
     1501 agttagttga attcccgctg gggccaacaa taaacgcccc ctctgaagat atgcgttttt
     1561 gcgcagcagt agctgcatat gggcaaaagt tacgcggttc tgaatacctg aacaatacct
     1621 cctggcagca gatcaaacag tgggctcagc aggcaaaagg ggaagatcca cagggttaca
     1681 gggcggaatt tattcgcctg attgaactgg cggatggtgt gactgacatc agtcagtgat
     1741 gactgtttag caaactatgt tcgaccagtc agcatatttg ctgactggtc gaattgatta
     1801 acaatgatgt taactcactc ttttgccgga tgctctattt ttgggttgtg agtgttttat
     1861 ttctaattgt gtataagaag cattgattcg tgcatcgcca tatttatcta aataacaatt
     1921 tctgtttacc agtcgatgtt gaaaatcaat ttcgtactgc tcttctgctg aat
//
LOCUS       DAZFRJ010000384         1962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000384 DAZFRJ010000000
VERSION     DAZFRJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.387"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..111)
                     /locus_tag="ACHKUN_004932"
     CDS             complement(<1..111)
                     /locus_tag="ACHKUN_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6663019.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNT"
     gene            complement(263..862)
                     /locus_tag="ACHKUN_004933"
     CDS             complement(263..862)
                     /locus_tag="ACHKUN_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6663020.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDDPKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYDGSGSGDWRSDAFIVGIGYRF"
     gene            complement(930..>1962)
                     /locus_tag="ACHKUN_004934"
     CDS             complement(930..>1962)
                     /locus_tag="ACHKUN_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6663021.1"
                     /translation="FFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQ
                     TVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAG
                     MQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFS
                     LTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFP
                     RDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKN
                     GAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMK
                     KATAGITIS"
BASE COUNT          460 a          555 c          445 g          502 t
ORIGIN      
        1 cgtgttcacc accaccgtgc tgctggttcg tctggccttc agcacaatgg tgcagttctg
       61 tactggtttt cctgtgccat ctttaagcac gccagaaatt tttactgtca tacttttcca
      121 ccaataaaaa aagcccgcag cagtgacgcc acgggcttca ggacagtgta actttacgtt
      181 tcctcaaacg cagttcaccc cataaggtgg atgaacctgc gtatcataac aatatttaca
      241 gaagataaat cggcgtctgt tgtcagaaac ggtatccgat accaacaata aatgcatccg
      301 atcgccagtc gccactaccg gaaccgtcat aagcaaggtc aatggtcacg gattcggtcg
      361 ggttaaactg cacgccagcc ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac
      421 ttccggtcag cacatcgtgc gttttcccct tgttgtcagt tacgcggaga taatccccgg
      481 agaaagtcga cacacggctg taagccacac ccgccatcgc atacgcgctg aaccattcat
      541 tcacgcgtac agacggcccc gccatcacgc tgaaccagcg gttacgcacg gaatcttcat
      601 gccagcgggt atcgctgtag cgcgtttttg ggtcatcctc agcattggca taactgaagg
      661 acgtaatcag ccccagcgcg tccataaact cataacggta tttcacgtta atcccgttca
      721 gatcatcact accgggaacg ttcgtcgagg catggagata ccccgcgctc agcgtggact
      781 gatgttctgc tgcactcgct ggcgtagcag cggcgacctg ccagactact gcggacaaaa
      841 taacagcaca taatttacgc ataattacct ctcgcttttc tgcaataaaa aaggcgccat
      901 ttctggcgcc cgtatctggg ttataaaatt cagctaatcg tgatgcctgc agtggctttc
      961 ttcatcacca caaccagcaa atcgctgata cttgctgtgg gataccagtt atttaccagc
     1021 catgctgaca ccgaaaactc cagtgtcatg tgaccgtgac cggcaggcat atcaataaca
     1081 ccactgtaaa tcagcgtatt atccagcgcg gtacggttat aaatttcagc accgtttttc
     1141 cgcactatca gacggcatga ggagtaaata tcagtatgct ctttctcatg tttagcgccg
     1201 ctgaatgcca ccgccggaat aacaatctgc cggtcaaacg gctgatcgtc ataaaccctg
     1261 acggtaatgg ttcctgatgg ccaccgctcc ggtgcacggg agtcccgggg gaaagctttg
     1321 cccactgttt taacgagatc gccttcaatc tggttcgcgg acaattttcc cagaacccga
     1381 cagttctcgt taatcgtgac gttgttgagc gtcccggagt tcgcattcac gttaccgctg
     1441 atatcggcat ttttcgccgt cagccgcccg tccggtgtca gggaaaatgc cggaggatta
     1501 ccgccgctgg taatggtggg agccgtcaga tatttcagga acacttcatt cataaatatc
     1561 tgatcgccct gaccaacaaa catcggcttt gtgttgccat tcgcaggatt aatcatcgca
     1621 atcctgtctg ccgccagcag cacctgactc tgcattcctg ctggcgtatt ctcaataccg
     1681 gcaccgatac ccgcaatata aaggcgtccg tcctgcatct gctgcagttt cacggcccac
     1741 atgctgttca ggttattatt tgtatcaacc tgaactttct gtatctgctg gattgccgca
     1801 ctctgatttt ccagtttttt attgacggtc tgcgtgattt cattgctgac attcgtaatg
     1861 gacgtcctga tttcagccag gtccggcgca agctgaccgt tatcaatctg cgtccacagc
     1921 tcctgggcca gatgtgtttt cccgatttct cctttgaaaa aa
//
LOCUS       DAZFRJ010000385         1953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000385 DAZFRJ010000000
VERSION     DAZFRJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.388"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            108..1460
                     /locus_tag="ACHKUN_004935"
                     /pseudo
     CDS             108..1460
                     /locus_tag="ACHKUN_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1473..>1953
                     /locus_tag="ACHKUN_004936"
     CDS             1473..>1953
                     /locus_tag="ACHKUN_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO6663022.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     "
BASE COUNT          638 a          378 c          362 g          575 t
ORIGIN      
        1 accttgctct tcttataaag aagggaacaa ttaagaatat taaatcttca aactttcatc
       61 aacttcacat caagaaatta aaaaatttaa acattaatct aaataataac aacctgatca
      121 cataatcggg tacgatttta tccgataact ataatgctat aactctggga aatggttgga
      181 atacaccgct gggtgccatc tctttttacg ctgcccgatc gagtagtaag ttaaataatg
      241 ataccagaca cgaaggtacg agctatcagg tcgcctataa taaatatcta gtacaaacag
      301 ccactcgatt tagcgtggcc gcctggcgtt atgcttcgca ggattacagg acattcagcg
      361 accatcttta cgaaaatgat aaacactatc atcagagtga ttatgatgat ttttatgata
      421 ttggcagaaa aaatagcctt tctgctaata ttatgcaacc tttatccaat aatctgggaa
      481 atgtatcatt aagtgcgctt tggcggaatt actgggggcg aagtggaaat gctaaagatt
      541 accaattcag ttactccaat aactggcaac acattagtta tactttctct gccagccaat
      601 cttatgatga aaataataaa gaagaggagc gttttaatct gtttatctcc attcctttct
      661 actgggggga tgatattgcc aaaacacgtc accaaattaa cttatcgaat tcgacctcat
      721 tttccaaaga tggctattcc tccaacaata ctggaattac tggcatagcc ggtgaacatg
      781 atcagttaaa ttatggtata tatgttaatc agcaacaaca aaataatgat acctcgcttg
      841 gtacgaattt aagctggaga actcccatcg ccataataga tggcagctat agtcattcta
      901 aaaacgcctg gcaaagtggt ggaagtatta gtagtggatt agttgtctgg cccggtggta
      961 ttaatatcac taaccagtta tccgatacat ttgcaattct ggatgcgcct gggttagaag
     1021 gtgcacatat taatggacaa aaatacaatc gaacaaacag caaaggccag gttgtttacg
     1081 acctgataat acctcatcgt gaaaaccatc tggtacttga tatagcaaac agtgaaagtg
     1141 aaacagaatt gcagggcaat cgtcaaatta ttgcacctta ccgtggagca gtttcttatg
     1201 tgcagtttac aactgaccaa cgtaagccct ggtatataca ggcactgcgt cccgatggtt
     1261 cgccattaac ctttggctat gacgtactgg atctccagga aaataatatt ggagtcgttg
     1321 gccagggtag tcgccttttt attcgcgtag atgaaattcc cactggcata aaagttgctc
     1381 tcaatgatga acagaactta ttctgtacta ttacttttca acacgttatc gatgaaaaca
     1441 aaacatatat atgccagtaa aaggagttta ctatgaagtg gcttttatta atcaccttga
     1501 gtctatattc ttttattgtg caaagtgctc cctgtgcatt gaccaatgtc ggtgaacaac
     1561 gtggaacata tatactcaag ccactgagta tgaaaggtaa tctaaccgcc gaaaaaattg
     1621 aaaacaccgg aaatatcatt gagtgtacag acttgcaaga gacaaccaac gccattaaag
     1681 ttcatttttt aacacagaac gctttaaggt ttaaagtcgg aagtcgtcat tatattatta
     1741 atttcccctt tgaatctgac ggtaaaaaaa tagagcttcc agggaagtca tcttataatt
     1801 tagaagaaat gctatctgcg ataaatttca ccattccata ttctatcgca tctgtatcag
     1861 atagttctaa caccgcagac atttttcctg gaactccttt tcctttgttg ttcgaaattg
     1921 aagtgagttc atgtacaggg aataatacta att
//
LOCUS       DAZFRJ010000386         1945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000386 DAZFRJ010000000
VERSION     DAZFRJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.389"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..153
                     /locus_tag="ACHKUN_004937"
     CDS             <1..153
                     /locus_tag="ACHKUN_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HFO6663023.1"
                     /translation="EDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANTLR
                     RDLMGR"
     gene            165..>1945
                     /locus_tag="ACHKUN_004938"
     CDS             165..>1945
                     /locus_tag="ACHKUN_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO6663024.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEP"
BASE COUNT          503 a          402 c          563 g          477 t
ORIGIN      
        1 gaagattttt tgaaagagct gatgcgtctt ggtcgcctgc agaaacatct gtttgtggaa
       61 ggaaaacgca cgggagataa agagtatgca gaggttctcg ttgctattac agagcttgct
      121 aatactctgc gaagagattt aatgggacgc tgagataatc gctgatgaat gccatcattc
      181 cgaaaaaaag acgcgacgga aagtcttctt ttgaagacct gattgcgtac acctcagtgc
      241 gggatgatgt gccggaagat gaactcaggc cagacacgga aatgaaagct gagattccgc
      301 accggaaccg cttcagacgt cttgttgatt atgcaacacg tctgcgtaat gaaaaatttg
      361 tttccctgat tgatgtgatg aaagacggca gtcagtgggt gaatttctat ggtgtgacct
      421 gctttcataa ctgtttgtct cttgaatctg cggctgaaga aatgcagaaa gcagcagacc
      481 ttgctcattt ttccaaagat gatacagatc ctgtttttca ctatatactc tcctggcctg
      541 cgcacgaaag tccgcgttct gagcagcttt ttgactgtgt gcgtcacacc ctgaaatccc
      601 ttgagctgtc aaaacatcag tatgtggcag ctgttcatac agacacggat aacctgcatg
      661 tacatgttgc agtgaatcgt gtccaccctg aaaccggcta tatcaattgc cttccgtgga
      721 gccaggaaaa actcagtcgt gcatgccgtg aacttgaaat taagcacgga ttcgcgccgg
      781 ataatggctg ctttgtgcat gcaccgggca atcgtattgt gcgcaaaaca gcgctggtac
      841 gtgagcgccg taatgcctgg cgacgcggga aaaagcagac gttcagggaa tatattgttc
      901 agatgtcgat tgccgggctg cgtgaagagc ccgcacagga ctggttatct ttgcataaac
      961 ggctggccag tgatggtctg tatatcacga tgcaggaagg ggaactggtg gtgaaggatg
     1021 gctgggacag agcgcgggaa ggtgttgcac tcagttcgtt cggaccgtta tggacagctg
     1081 aaaaactggg gcggaaactg ggggaatatc agcctgtacc gacagatatt ttcagtcagg
     1141 tggggacacc cggtcgttat gatccggaag ccataaacgt tgatatccgg ccggaaaaag
     1201 tggcagaaac ggagagtctg aaacagtacg cctgccgtca ttttgccgaa cgcttaccgg
     1261 aaatggcgag aaatggtgag ctggaaagtt gcctggatgt gcacagaact ctggcaacgg
     1321 ctggattatg gatggggatc cagcacgggc atctggtact gcatgatgga tttgataaac
     1381 aacagacacc agtacgtgca gacagtgtgt ggccgttaat gacgctggat tatatgcagg
     1441 atctggatgg tggatggcag cctgtaccga aggatatttt tactcaggtt atacccggag
     1501 aacggttccg gggacgtaat cttggtactc aggctgtcag tgattacgaa tggtatcgta
     1561 tgcggatggg gacaggtcca cagggagcaa taaagcgcga actgttttct gataaggaaa
     1621 gtctgtgggg atacggcctt attcagtgca ggagcctgat tgaagagatg atagctgacg
     1681 gggatttcag ctggcaggca tgtcatgaga tgtttgcgcg taaagggctt atgctgcaga
     1741 aacagcatca tggtcttgtg attgtggatg cgtttaatca tgagctgaca ccggtgaagg
     1801 ccagcagtat ccatcctgat ttgacgttat cccgtgccga gcctcaggcc gggccatttg
     1861 aaattgcggc tgcagatatt ttcgaacggg tgaagcctga atgtcgttac aatccggaac
     1921 ttgctgccag tgatgaagtg gaacc
//
LOCUS       DAZFRJ010000387         1945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000387 DAZFRJ010000000
VERSION     DAZFRJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.390"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..153
                     /locus_tag="ACHKUN_004939"
     CDS             <1..153
                     /locus_tag="ACHKUN_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HFO6663025.1"
                     /translation="EDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANTLR
                     RDLMGR"
     gene            165..>1945
                     /locus_tag="ACHKUN_004940"
     CDS             165..>1945
                     /locus_tag="ACHKUN_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO6663026.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIDQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDKAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVVPGERFRG
                     HSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEP"
BASE COUNT          501 a          410 c          558 g          476 t
ORIGIN      
        1 gaagattttt tgaaagagct gatgcgtctt ggtcgcctgc agaagcatct gtttgtggaa
       61 ggaaaacgca cgggagataa agagtatgca gaggttctcg ttgctattac agaacttgct
      121 aatactctgc gaagagattt aatgggacgc tgagataatc gctgatgaat gccatcattc
      181 cgaaaaaaag acgtgacgga aagtcttctt ttgaagacct gattgcgtac acctcagtgc
      241 gggatgatgt gccggaagat gaactcaggc ctgacacgga aatgaaagct gagattccgc
      301 accggaaccg cttcagacgt cttgttgatt atgcaacacg tctgcgtaat gaaaaatttg
      361 tttccctgat tgatgtgatg aaagacggca gtcagtgggt gaatttctat ggtgtgacct
      421 gctttcataa ctgtttgtct cttgaatctg cggctgaaga aatgcagaaa gcagcagagc
      481 ttgctcattt ttccaaagat gatacagacc ctgtttttca ctatattctc tcctggcctg
      541 cgcacgaaag tccgcgttct gagcagcttt ttgactgtgt gcgtcacacc ctgaaatccc
      601 ttgagctgtc aaaacatcag tatgtggcag ccgttcatac agacacggat aacctgcatg
      661 tacatgttgc agtgaatcgt gtccaccctg aaaccggcta tatcaattgc cttccgtgga
      721 gccaggaaaa actcagtcgt gcatgccgtg aacttgaact taagcacgga ttcgcgccgg
      781 ataatggttg ctttgtacat gcaccgggca atcgtattgt gcgcaaaaca gcgctggtac
      841 gtgagcgccg taatgcctgg cgacgcggga aaaagcagac gttcagggaa tatattgatc
      901 agatgtcgat tgccgggctg cgtgaagagc cagcacagga ctggttatct ttgcataaac
      961 ggctggccag tgatggtctg tatatcacga tgcaggaagg ggaactggtg gtgaaggatg
     1021 gctgggacaa agcgcgggaa ggtattgcac tcagttcgtt cggaccgtca tggacagctg
     1081 aaaaactggg gcggaaactg ggggaatatc agcctgtacc gacagatatt ttcagtcagg
     1141 tggggacacc cggtcgttat gatccggaag ccataaacgt tgatatccgg ccggaaaaag
     1201 tggcagaaac ggagagtctg aaacagtacg cctgccgtca ttttgccgaa cgcttaccgg
     1261 caatggcgcg caatggtgaa ctgaaaagct gcctggctgt acaccgtact ctggcagagg
     1321 cgggattatg gatggggatt caacacgggc atctggtact gcatgatgga tttgataaac
     1381 aacagacacc agtacgtgca gacagtgtgt ggccgttaat gacgctggat tacatgcagg
     1441 atctggatgg tggatggcag cctgtaccga aggatatttt tactcaggtc gttcccggag
     1501 aacgattccg gggacacagt attggctctc agcctgtcag tgattacgaa tggtatcgca
     1561 tgaggatggg cacaggtccg cagggggcta taaagcgtga actgttttct gataaggaaa
     1621 gtctgtgggg atacggcctt attcagtgca ggagcctgat tgaagagatg atagctgacg
     1681 gggatttcag ctggcaggca tgtcatgaga tgtttgcacg taaagggctt atgctgcaga
     1741 aacagcatca tggtcttgtg attgtggatg cgtttaatca tgagctgaca ccggtgaagg
     1801 ccagcagtat ccatcctgat ttgacgttat cccgtgccga gcctcaggcc gggccatttg
     1861 aaattgcggc tgcagatatt ttcgaacggg tgaagcctga atgtcgttac aatccggaac
     1921 ttgctgccag tgatgaagtg gaacc
//
LOCUS       DAZFRJ010000388         1930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000388 DAZFRJ010000000
VERSION     DAZFRJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.391"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1144
                     /gene="escC"
                     /locus_tag="ACHKUN_004941"
     CDS             <1..1144
                     /gene="escC"
                     /locus_tag="ACHKUN_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO6663027.1"
                     /translation="KNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQSKAKN
                     KDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVERNGNL
                     FDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQIEISVSIIDVDANDLQQ
                     LGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRVNALQQNSKAKILSQPS
                     IITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDNSNSLTGKRRE
                     RVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLSAGESLLLGGFIQDKES
                     SSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKSASSE"
     gene            1158..1613
                     /gene="cesD"
                     /locus_tag="ACHKUN_004942"
     CDS             1158..1613
                     /gene="cesD"
                     /locus_tag="ACHKUN_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO6663028.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
BASE COUNT          637 a          370 c          380 g          543 t
ORIGIN      
        1 taaaaactcc tgtaacgtga gaaaaatcac aacatttaac tcgattgaag ttcgtggcgt
       61 tcctgaatgc ataaaataca ttacatcttt atctgaaagt cttgataaag aagcacagtc
      121 taaagcaaaa aataaagatg tcgttagagt atttaaatta aactatgcat cagcgacaga
      181 tataacgtat aaataccgcg accaaaatgt tgtcgtgcct ggcgtcgtct ccatcctgaa
      241 gacgatggca agtaatggct cacttcccag cacaggcaaa ggtgctgtag aaagaaacgg
      301 caacctgttt gataacagtg taaagatttc cgcagatcca cgtcttaatg cagttgttgt
      361 aaaagatcgt gaaataacta tggacatata tcggcaatta atttctgagc ttgatattga
      421 gcaaaggcaa attgagattt ctgtttcaat tattgatgtt gatgcaaatg atcttcagca
      481 actgggagta aactggtccg gtacattaaa tgcagggcaa ggttctattg cctttaacag
      541 ctcaacggcg caagctaatc tcagttcttc tgtgataagc aacgccaata atttcatgat
      601 cagagtgaat gcgttacaac agaactcgaa agcaaaaata ctctctcagc catcaattat
      661 aaccttaaat aatatgcagg cgattcttga taaaaacgta acgttttata caaaagtttc
      721 cggagaaaag gtggcatctc tggaaagtat taccagcgga acattactga gagtaacacc
      781 acgtatactt gatgacaact cgaatagtct taccggaaaa cgtcgggaac gagttcgttt
      841 gttacttgat attcaggatg gcaatcaatc gactaaccaa agtaatgctc aggatgccag
      901 cagcatctta cctgaagtac aaaattcaga aatgacaaca gaagcgacat taagcgctgg
      961 agaaagtctg ctgcttggcg gatttatcca ggataaagag agttcaagta aagatggtat
     1021 accgttgtta agtgatattc ctgttattgg taacttattc tccagtacaa caaaacaaaa
     1081 acacagcgtt gttcgactct ttttgattaa agcgaccccg ataaaatcag catctagcga
     1141 ataataaatg aggctttatg agcaggaaat ttagctctct acaggatatc tatgatttct
     1201 accaggatgg tggtacgtta gcatcactga caaatttgac gcaacaagat ctcaatgatc
     1261 ttcattctta tgcctataca gcatatcaat ctggtgatgt tataaccgca agaaatctat
     1321 ttcatctgct aacatatctg gagcactgga attatgacta caccctatct ctggggttgt
     1381 gtcatcagcg tttatcaaat catgaagatg cacaactgtg tttcgctcgc tgtgcaactt
     1441 tagttatgca agatcccagg gcatcttatt attctgggat tagctactta ttggtcggaa
     1501 atcggaaaat ggccaaaaaa gcctttaagg cttgtttaat gtggtgtaat gaacaagaaa
     1561 aatacgctac atataaagaa aatattaaaa aattgttagg taatacagat taaaaactac
     1621 catctgccat accaatcccc aggctgtctg gggattttct atcggaaata ataaagctgc
     1681 tttggtgaag tgcgcccaac gttgagcacc gaccgctggc agatttatct acaggagtgg
     1741 gttactccat taaccaggat tttatgcgcc tgaaccatct tctgctgtac tcaatgtcat
     1801 ttccgcttat agaataagca gtatatatct taacttagca tcagaaatct gactgtttca
     1861 atcatctata gcccagagtg tatttgtcag gaaatgactt tcaaactttg gcagagaaaa
     1921 aggcttatcc
//
LOCUS       DAZFRJ010000389         1927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000389 DAZFRJ010000000
VERSION     DAZFRJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.392"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..447
                     /locus_tag="ACHKUN_004943"
     CDS             <1..447
                     /locus_tag="ACHKUN_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFO6663029.1"
                     /translation="NIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVIDWYNPQKNA
                     FGIMMGTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVS
                     ASVGSLLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(460..681)
                     /locus_tag="ACHKUN_004944"
     CDS             complement(460..681)
                     /locus_tag="ACHKUN_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663030.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(765..1145)
                     /locus_tag="ACHKUN_004945"
     CDS             complement(765..1145)
                     /locus_tag="ACHKUN_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663031.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1346..1921)
                     /locus_tag="ACHKUN_004946"
     CDS             complement(1346..1921)
                     /locus_tag="ACHKUN_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO6663032.1"
                     /translation="MPLTSDIGSRSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TNGRFSSSGGNDGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQESIQDKISQCK
                     FPVSSGNFQCPPESIQCPITLERPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSVHP
                     LTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          620 a          421 c          338 g          548 t
ORIGIN      
        1 aatataaaac gggatttcgt tgctgtaaga atccaaagta atcagtttac tgatttaaaa
       61 aacaagacaa ttcagggtca taaagacact gtcgcaaaag tcatagactg gtataaccca
      121 caaaaaaacg catttggtat catgatgggc acaccaagac gttctgctga tatagcaaag
      181 gaagaatcca gaaacgctct aaatttcatg ataatggaaa aggatacatt taatgaaaag
      241 atacttaaca gcaatgctaa tttacaaaaa aaatacggca caacagaaaa tagtagttgg
      301 gtatcggctt ctgttggttc tcttctggac aaaggagcca aagtatatcc agatatatca
      361 tgctctctga gactgggaga accattcatt attacacttc cagaaacagt caggctggat
      421 gttaatatcc acccactcaa aaaatgatcc tgaacgtttt tatgcaggca aatgcgtcca
      481 catcaaggct tcaaccttgt gctctccccc tgtcacagga taaatttgcc tgtaaaaatc
      541 aaactctccc tgctcattac caataccaat cagttgaatg cagttgccat ctacaataaa
      601 tttttccgct gacgtaacca aaataaaggc ttccggatgt tcattaggaa aggtgttagc
      661 aagagtacac tgaatatcca tctccaacaa aatctcctga ttattagcgt aaactcatcg
      721 acgaaatcgt tactatcccc tgggcacata tgtaccattg aatactactg ttctccagat
      781 tctgataatc catggcgaac aataagaaca ctacagccgc tttgctgatt agttagccat
      841 aacggtgaac tttgctggat agtttgagaa atatcatttc gccggaggct tatacactga
      901 ttactcaagc ttacaactgc aggtgcatca aagcgctccg gactatcagt atcacgcata
      961 gctgtaaaca gaatataatc actaccagcc atatcatatt ccgtttcttt tagtcttaac
     1021 aagccatcct ctcttgtaat attaactgta attaaagatg tctcatcatg atgggcatag
     1081 cgctgtgtat taagaattaa atgctcttcc agaaatttta cagggttact acgtatttca
     1141 ttcattatac accgaagctc tgaacgatgc ggcaccagaa agtgcccggt attttgtgaa
     1201 ataatactac ttaccttgat tgttttctct ccagctcatg aaaaataact cgcgtattat
     1261 attgttttac caaccacagg ccccgaatgc ctgtggtgtt tttatataaa ttatcggaag
     1321 ttaatattgt ttcatttgaa acatcttaac tatcttttat aataaagctt ccccttgcag
     1381 ggtcatattt acactcttct ggtttaacaa tcattgatgg ggttattgga gctcgtgtca
     1441 gagggtgaac tgaaccgtca ttaaccacgc gagaaagtgc atcaacatca aataaagtgc
     1501 atactgctga actatctgaa tttttgacaa acactccttc ttcgggtctc tctagtgtaa
     1561 ttggacactg aatagactct ggcgggcact ggaaatttcc tgaactaaca ggaaacttac
     1621 attgagatat tttgtcctga atactctctt gcgattgtgc ttcacctgag tccgaaagca
     1681 tacttagcat tctctcacta agggctcgag gaccattatt aaatcccaaa atcaataact
     1741 cagaaagcaa tccgtcatta ccgccactgg atgaaaagcg cccattagta gcatcataca
     1801 caatactgac agtttcacca cctactgtaa tatctccgcg cccattggct gcaattcggg
     1861 cacgaaaaac ctccagccca agattaaatg aacgtgatcc aatatctgag gttaatggca
     1921 taacaag
//
LOCUS       DAZFRJ010000390         1902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000390 DAZFRJ010000000
VERSION     DAZFRJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.393"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..553
                     /gene="espM1"
                     /locus_tag="ACHKUN_004947"
     CDS             <1..553
                     /gene="espM1"
                     /locus_tag="ACHKUN_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO6663033.1"
                     /translation="GISYHKDNSFRGTIRGKNDEVVKCSMGERSIRFNVNKFSGCILE
                     TVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEERKMVFSLVSKEFE
                     LTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYIANVLADKFYQKHI
                     APGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(772..1828)
                     /locus_tag="ACHKUN_004948"
                     /pseudo
     CDS             complement(772..1828)
                     /locus_tag="ACHKUN_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          508 a          394 c          464 g          536 t
ORIGIN      
        1 tggtatcagt tatcataaag ataattcttt ccggggcacc atccgtggga agaatgatga
       61 ggtcgtgaag tgttcaatgg gagagcgctc tatccgcttt aatgttaaca aatttagcgg
      121 ctgtatactg gagacggtaa gcaggcagag tactaaagat attcatggat gggtaagtga
      181 tgaacggaca gtatatccgt caagggttat caaccaagag attgataatt gctgccttca
      241 aaaaaatgca aaaatttctt ctgaagagag gaaaatggtc ttttctcttg taagtaagga
      301 gtttgaacta actcttgatg ttaaagcggc acaaagttct attaatcata taataatagg
      361 aaatgcttct tttggcaaaa aaatggatgc tctttgcgat ggtatgagcc gagctgtaaa
      421 aaacagtaca acagattaca tagcaaacgt gcttgcagac aagttttatc agaaacatat
      481 cgccccgggt gttgatattg taaaactacg aaatgaaatc ccaggttata tgagtcgtgt
      541 tatacagggg taaggttgga ggaggctatg cgccagtatc attggttttc atcctgtaaa
      601 aagatatgag taaaatatac aacaggcgtt acactatatg tatgtatcca aataactcat
      661 agttcacctg tacagttata aatcgtaaag aaaattgcag cgcgtcgtac gcagaaacgt
      721 gctgtgattg actatttata gctttgtcac aacgacaaga ttcagtagtg tttacacggg
      781 cgggagaaat gacggaataa ctaaatagct gcgcctaata tcgcacattt ttgccagccc
      841 atgtttgcct tcggggaatg gactgacgga ttccataaaa tggcgaactt tttcaatagt
      901 tgccacattg agcggcactg atgattacgc gttattgtgg cgtgaagtat ctgccatagc
      961 tattcaaaat gattcaccca tggagattaa accggctgat aaatgactct gcacttcggt
     1021 ttatccttca gccagcgctg ggtttcccgg cctttgtgga taatgtagtt gtcctcgagc
     1081 agcgtgatgg ttttcgtccg acagtatgta gctttaagcc gcttcagcag tctgatgaac
     1141 agcgccgaac ttttgctgtt ggcgcccaca cagctgactt aacctgtccc gctgtgcagc
     1201 gctacgtcca gagaatattt ttcattttgt tccggcgtga cccctcgttt ttgcttcccg
     1261 agcagtttcc agtccgcacc gattttggga ttaagatgga taaatacttc atcttcataa
     1321 aagaccggat gctctgtgct gcattcgtcc agtgcgttat ggattgttgc catcttttta
     1381 tctttaggcg gggcacggat acgtagagtt ggcgcagccc ttcgccacac aagccccaca
     1441 gacgacaacc agcggcgaat ggttccagca tgtaacaggc agccggttat ctcattgatt
     1501 tttattgtca gcagttctgt actccagcgt gaataattgt agccaaagtc gcgagtgaat
     1561 attttacctg ttcacgtaat agtgtgtaga tatgcgcaaa cggccagcgg cgggctcgct
     1621 cggcaggtaa tgatttcagt ccctcaacac ccgactgtgt gaactagtta atccagcgcc
     1681 caacagagga acgggcgcag catagtgtgc gggcaacgtc gctgacacgg tcgccccggt
     1741 gcagcatcag catggctgtc agccttttat ggatggcttt ctgtattagg cgtcgttcgt
     1801 cacgggaaat tggtggtatg atcgacattg ctcagtccgg ttggtgattt gtattgattt
     1861 ggcgattgat cagatcgcac aatcgggttg agttccctct aa
//
LOCUS       DAZFRJ010000391         1860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000391 DAZFRJ010000000
VERSION     DAZFRJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.394"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..464)
                     /locus_tag="ACHKUN_004949"
                     /pseudo
     CDS             complement(1..464)
                     /locus_tag="ACHKUN_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(473..1240)
                     /gene="sctT"
                     /locus_tag="ACHKUN_004950"
     CDS             complement(473..1240)
                     /gene="sctT"
                     /locus_tag="ACHKUN_004950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO6663034.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1242..1502)
                     /locus_tag="ACHKUN_004951"
     CDS             complement(1242..1502)
                     /locus_tag="ACHKUN_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6663035.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1512..>1860)
                     /locus_tag="ACHKUN_004952"
     CDS             complement(1512..>1860)
                     /locus_tag="ACHKUN_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6663036.1"
                     /translation="SEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEI
                     KSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSK
                     GLILQYFDLSINP"
BASE COUNT          651 a          379 c          309 g          521 t
ORIGIN      
        1 tgattactcc aaaatacttt gatcgccaat gcaaaaaaaa caatataaag aattgcttta
       61 acaaattctt ttacggtttt taagccaaat atacgcttta agccatttac tggattcaat
      121 gaatcaaact taaatttaac agcttcagtt gctaattgca ttttactttg aaaccaggag
      181 acaagtattg ttgtgaacat gcaaaccagc aaaaaaggaa tgttcgtttt caaccaacca
      241 acccccatag ctttaaaata gtcccaaata tctggtttag cgttatgatc aaggatgtat
      301 tcaagaatcg acataatgtg atgcacatca aaaacatatc cgagatataa agtacccaca
      361 agcattatta cagagatggt taagtctcta ctttatagaa attgcccttt tttagaagca
      421 tcctggagtt tcttttgtgt tggcttttca gttttattgg ccatgaaaca ctctatttct
      481 caatgtatag ttgcaattgt tctgggaaaa aggataacgg cattactcgt tcagccagta
      541 ttggggcaaa ataaataatt aatattaaaa atgccagcgc acttttgaca gtcagtgaaa
      601 tggcaaaagc atttagttgt gaagcataac gtgccagcaa tcccaggaca gcctcaccgc
      661 ccagcataac ggcaatcacg gggcttgcgt aaacgatagt atgagtcatt atgttattaa
      721 tatatgagaa taaaggctct aatttaggaa aattaaaatt gcctgaaggc cataaattat
      781 aactttgcca aagagtttcc agaataaagt tcattccacc tttagtaaga taaaccgcag
      841 cagagaaaag gttaaataat cttgctaatt ccgaggtatc gacacctgta gccggatcca
      901 aagtactact taatgttgca ccgcgttggt tatcgataaa gctacctata gcaaggaaaa
      961 tccaaaacgg ggatgctaat aagcatgcaa taaacagccc tatgataatt tctctggcag
     1021 cgaacaaagc aatatcgatt tcatttaagt tcaataattc atagtggtaa tgctgaacta
     1081 atgcacatga taccactatt attattgggt gacgaactac tgttggtata ttcccactgg
     1141 cgaaaaatgg taacaaatag aaagttggtg ccaatcggca aaaaccgagc gctgtagcag
     1201 ccagcagact atgcaactga tagagtatcg cctcccccat gctatcctct cgcgaatgct
     1261 aaattcagca tttcgatacc aaatgaatat aatttttcac cataccaccc gctcattaaa
     1321 aaaaagcaaa tacttacgca caataatttc acgccaaatg gcaacgtctg ttcttgcaac
     1381 tgcgttaccg tttgaaaaag ccctaccaat aaaccaacaa atgtcgcaac tgcaataggt
     1441 cctgcagaca taactaaaat cagatacagc gccctattcc ccgcaaaaac aatatcgtcc
     1501 atattttttt ctcagggatt aatggatagg tcaaaatatt gtaaaatgag cccttttgat
     1561 aacattgtcc aaccgtccat cgcgacaaac aaaattaatt ttatcggtgt agatatggtt
     1621 accggactca tcatcatcat acctaacgtt aacagaacgc tggatataac cagatccact
     1681 acgacaaagg gtaaatagat ataaaaacct ataataaatg cactttttat ctcactcaat
     1741 gcatatgcgg gcagtaacga gaaaattgaa atattatcat catcgattat ctcttcattg
     1801 tcttcagaac tattcacctt ctgaatcttt tcaaaaaagc taaccaactc aggttcagaa
//
LOCUS       DAZFRJ010000392         1855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000392 DAZFRJ010000000
VERSION     DAZFRJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.395"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            11..712
                     /locus_tag="ACHKUN_004953"
     CDS             11..712
                     /locus_tag="ACHKUN_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6663037.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            709..909
                     /locus_tag="ACHKUN_004954"
     CDS             709..909
                     /locus_tag="ACHKUN_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663038.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            1108..1289
                     /locus_tag="ACHKUN_004955"
                     /pseudo
     CDS             1108..1289
                     /locus_tag="ACHKUN_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1295..>1855
                     /locus_tag="ACHKUN_004956"
     CDS             1295..>1855
                     /locus_tag="ACHKUN_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663039.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMP"
BASE COUNT          595 a          391 c          408 g          461 t
ORIGIN      
        1 tggtgatgat atggaagcct tacaaacaac accaaaaaca tgtagcctca atacttacaa
       61 caaggtttta tgtgatgatt tagatctcga ttcttttgca ttaaccatcg caaacctgct
      121 cagtgctgtt cgcaccttca acctcctaga tgatacgcga ttaaaagagg ttgggtttga
      181 tgtgctagaa tttactcatg aatatgcttt agcgatcgca tcgacaaaac aacaacattc
      241 tatccgatca ggcaacaaga tagcctgcat acgcaccaaa cgtgaagcct gcggcttgac
      301 gactaccgaa ctagccagac tactcgatct tgacgaagag attattctgc aatgggagag
      361 tggagagttc gagccaacca tcagcatgct tatccccctg gcaaacgtcc tgggatgcga
      421 tccgctttct ctactaagtg aaaaaaacag cgagtcagct attcgtgtaa atacgactga
      481 agtccatgcg gaaagtattg gcgcacgcat caaaagcgcc cgtacaaagc tgggattaac
      541 cgaatctgat cttgcccgca tgattcatac ctacagtgac cccataaacg actgggaatg
      601 cggcatctgt gaagttcctg ctgatcatat agtaccactg gccagtgcgc ttaattgtga
      661 cctgatgtgg ttgttaacgg gaaaatcaga agcaaaggag cagcaacaat gactggcaat
      721 atccatgata agtatgaagg cttatgcctg gcaccggatt cctttgcaaa caatatccat
      781 aatttattat gcgcagttat cgtattacaa atgtcagaca acgacgcaat aaaaagaaca
      841 ggtgatgaag ttcttgaatt tgcacgttgc tatgctgaag cagcagctga aaaagaacta
      901 tccagttaaa tagaacaagt catctccaga tactatatta cggcttaatc gccggggatt
      961 atcacacact taatccacag gaggttttat tatgactttt ataaaacata aggcatcaca
     1021 caaaacagcc tgccttattg cacaacacgg gaaaaatcac atgcatattg cctgcttgtt
     1081 tctgcgtaaa gcatacggga gataataatg catcagaaaa cagcagaaca cgaacaaacc
     1141 agagtattgc tgaccatcaa aaacgggaaa gtaatattaa ttcgtcatgt tcatgacgat
     1201 gaatttgtag aaatctttca acattcctgt ttattgcaga aaaggcagga tatgacgtta
     1261 ttgcaccagc agatgaagat gaggaataaa tttcatgcaa tacgctgaat tccaggctga
     1321 agcaacagcc aatggtatac aaacaggcag tatgactatt gattatcacg acgccatacg
     1381 ccgtctggat gctggagaat tcgatactcc taatgtgcga ggtttacgta tccttcagtg
     1441 tctcgcgcaa gctgacgaag caggattact gggaaaactt ccggttgaga tgaaggttgc
     1501 ccagtggcga tggttgtatg tgacgacatt catcaacgaa gaagaagaca agaacggcac
     1561 aattgatgtc ccgaatgaac acggaacaac agatcgcgcc gtaatatata acgggaagca
     1621 tggggttatg acgatatatc ccggccccat tcggtttgcc ttacagcagt atattgaatg
     1681 gaatttaatt caaaaatacg gcgaggcaga aggaatgggt agagcgctgt ttctttatca
     1741 gaaaatgctc attacatacc cagataaagg tttcattgtt tcagacatgg ggcgagaagg
     1801 gcttgaactc cttctggatg aaatgattaa cgacctgaat actcatggta tgcca
//
LOCUS       DAZFRJ010000393         1853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000393 DAZFRJ010000000
VERSION     DAZFRJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.396"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            201..395
                     /locus_tag="ACHKUN_004957"
     CDS             201..395
                     /locus_tag="ACHKUN_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663040.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            403..1815
                     /gene="pykF"
                     /locus_tag="ACHKUN_004958"
     CDS             403..1815
                     /gene="pykF"
                     /locus_tag="ACHKUN_004958"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO6663041.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          500 a          457 c          434 g          462 t
ORIGIN      
        1 cctcacacta acaactttgg caccagacgt tgcgcaaaca gcgaagtttt tgcgtaacct
       61 tttccctgga acgttaaatc tttgataaca atttattgtc taacaagttg tatatttttt
      121 gaaacgctgt ttttgttttc cttttggatt aatttcagcg tataatgcgc gccaattgac
      181 tcttgaatgg tttcagcact ttggactgta gaactcaacg actcaaaaac aggcactcac
      241 gttgggctga gacacaagca cacattcctc tgcacgcttt ttcgatgtca cctatcctta
      301 gagcgaggca ccaccacttt cgtaataccg gattcgcttt ccggcagtgc gcccagaaag
      361 caagtttctc ccatccttct caacttaaag actaagactg tcatgaaaaa gaccaaaatt
      421 gtttgcacca tcggaccgaa aaccgaatct gaagagatgt tagctaaaat gctggacgct
      481 ggcatgaacg ttatgcgtct gaacttctct catggtgact atgcagaaca cggtcagcgc
      541 attcagaatc tgcgcaacgt gatgagcaaa actggtaaaa ccgccgctat cctgcttgat
      601 accaaaggtc cggaaatccg caccatgaaa ctggaaggcg gtaacgacgt ttctctgaaa
      661 gctggtcaga cctttacttt caccactgat aaatctgtta tcggcaacag cgaaatggtt
      721 gcggtaacgt atgaaggttt cactactgac ctgtcagttg gcaacaccgt actggttgac
      781 gatggtctga tcggtatgga agttaccgcc attgaaggta acaaagttat ctgtaaagtg
      841 ctgaacaacg gtgacctggg cgaaaacaaa ggtgtgaacc tgcctggcgt ttccattgct
      901 ctgccagcac tggctgaaaa agacaaacag gacctgatct ttggttgcga acaaggcgta
      961 gactttgttg ctgcttcctt tattcgtaag cgttctgacg ttatcgaaat ccgtgagcac
     1021 ctgaaagcgc acggcggcga aaacatccac atcatctcca aaatcgaaaa ccaggaaggc
     1081 ctcaacaact tcgacgaaat cctcgaagcc tctgacggca tcatggttgc gcgtggcgac
     1141 ctgggtgtag aaatcccggt agaagaagtt atcttcgccc agaagatgat gatcgaaaaa
     1201 tgtatccgtg cacgtaaagt cgttatcact gcgacccaga tgctggattc catgatcaaa
     1261 aacccacgcc cgactcgcgc agaagccggt gacgttgcaa acgccatcct cgacggtact
     1321 gatgcagtga tgctgtctgg tgaatccgca aaaggtaaat acccgctgga agcggtttct
     1381 atcatggcga ccatttgcga acgtaccgac cgcgtgatga acagccgtct cgagttcaac
     1441 aatgacaacc gtaaactgcg cattaccgaa gcggtatgcc gtggtgccgt tgaaactgct
     1501 gaaaaactgg atgctccgct gatcgtggtt gctactcagg gcggtaaatc tgctcgcgca
     1561 gtacgtaaat acttcccgga tgccaccatc ctggcactga ccaccaacga aaaaacggct
     1621 catcagttgg tactgagcaa aggcgttgtg ccgcagctgg ttaaagagat cacttctact
     1681 gatgatttct accgtctggg taaagaactg gctctgcaga gcggtctggc acacaaaggt
     1741 gacgttgtag ttatggtttc tggtgcactg gtaccgagcg gcactactaa caccgcatct
     1801 gttcacgtcc tgtaatattg cttttgtgaa ttaatttgta tatcgaagcg ccc
//
LOCUS       DAZFRJ010000394         1847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000394 DAZFRJ010000000
VERSION     DAZFRJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.397"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            140..643
                     /gene="ftnB"
                     /locus_tag="ACHKUN_004959"
     CDS             140..643
                     /gene="ftnB"
                     /locus_tag="ACHKUN_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO6663042.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHKUN_004960"
     CDS             complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHKUN_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO6663043.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHKUN_004961"
     CDS             complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHKUN_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO6663044.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1437..1760
                     /gene="yecR"
                     /locus_tag="ACHKUN_004962"
     CDS             1437..1760
                     /gene="yecR"
                     /locus_tag="ACHKUN_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO6663045.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          481 a          432 c          408 g          526 t
ORIGIN      
        1 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
       61 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      121 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      181 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      241 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      301 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      361 tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      421 cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga
      481 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gtctgttgct
      541 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
      601 cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat
      661 gcattgcgcc gatgaaggtt ttgagaaacc gcagcctcat ctgtttgaag cagcggtttt
      721 tttaatggga ttcaccctgt ggggtaaact tgagttcaat aagcgcgatg gctttttgga
      781 ttgcctgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttca
      841 gctgactgga cattttttca cgaacttcaa cgggcgcgat tacatcgaga acatccagaa
      901 tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca
      961 gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca
     1021 catcttcagc gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccggagcagg
     1081 ttttttatta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat
     1141 tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac
     1201 aggtgttatt ctctttgcgc ttataatatg acaaccatca caaaaatcaa tctttatgtg
     1261 atacaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaaa aacagccccc
     1321 ctatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt ttccggccat
     1381 tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc tggaccatga
     1441 gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccagg
     1501 cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat gaccaggctt
     1561 ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc
     1621 agcaggaagg ctatacccac gcggtcccct ttggtcagcc agtaggcaac tgtagccttt
     1681 ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg
     1741 ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg attaaagaaa
     1801 tagcggtaat gcgttttatt cccattcgta tttttaataa ttgaagt
//
LOCUS       DAZFRJ010000395         1823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000395 DAZFRJ010000000
VERSION     DAZFRJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.398"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..853
                     /gene="rcnA"
                     /locus_tag="ACHKUN_004963"
     CDS             29..853
                     /gene="rcnA"
                     /locus_tag="ACHKUN_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO6663046.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1072..1410
                     /gene="rcnB"
                     /locus_tag="ACHKUN_004964"
     CDS             1072..1410
                     /gene="rcnB"
                     /locus_tag="ACHKUN_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO6663047.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1492..>1823)
                     /locus_tag="ACHKUN_004965"
     CDS             complement(1492..>1823)
                     /locus_tag="ACHKUN_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6663048.1"
                     /translation="LLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESN
                     GSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATA
                     LINVKYD"
BASE COUNT          452 a          425 c          419 g          527 t
ORIGIN      
        1 tgccgaatta atactaagaa ttattatcat gaccgaattt acaactcttc ttcagcaagg
       61 aaacgcctgg ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gcctggaacc
      121 agggcactca aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca
      181 ggcggtgatg ctcggactgg cagcaactat ttcgcatacc gcagtggtct ggttgattgc
      241 ctttggcggg atggtggtca gcaagcgctt tactgctcaa tcagcagaac cgtggctcca
      301 gttgatttcc gcagtgatca ttattagcac cgcgttctgg atgttctggc gtacctggca
      361 cggcgagcgc aactggctgg agaatatgca cgagcatgat catgagcatc atcatcacga
      421 tcacgaagat caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga
      481 tgctcatgca cgagcccatg ctaatgacat taaacgacgc tttgatggta gagaggtcac
      541 caactggcaa attttgttat ttggcttaac cggtggcctt atcccctgcc cggcagcaat
      601 taccgtgctg ttgatatgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt
      661 cagtttcagc attggcctgg cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag
      721 cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgcccccta
      781 tttttccagt ctgttgattg gcttagtcgg tgtgtatatg ggcgtacatg gcttcatggg
      841 cataatgcga taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt
      901 aaatcagcta tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat
      961 ttacactttt tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta
     1021 tggcaggaaa gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt
     1081 aaaaataaga tgttgctggg tgcgcttttg ctggttacca gtgccgcctg ggccgcacca
     1141 gccaccgcgg gttcgaccaa tacctcggga atttctaagt atgagttaag cagtttcatt
     1201 gctgacttta agcatttcaa accaggcgat accgtcccag agatgtaccg caccgatgag
     1261 tacaacatta agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg
     1321 acctatatgg gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc
     1381 tacgacggtg agatttttta tcatcgctaa aaaaagcccc ctcatcatga gggggaaatg
     1441 cagacacctt gttatttttt attattagcc acttgctcgt cttgcttgtt attagtcgta
     1501 tttcacgttg attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta
     1561 gacgtagtaa ctggcagtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc
     1621 cgcaatttct tttaaatttg tgtatcgttt actgctgtta gtgatattta aaagtgtgac
     1681 tgacgaacca ttactttcaa gaataaatcc tacgccatta tcttccgcac ctttcagtat
     1741 tgtgttgctg ttggttaaca agtcagaact tgataaatac acctgcattt tccgtgatgt
     1801 ttgattttgt tgacctaata aat
//
LOCUS       DAZFRJ010000396         1818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000396 DAZFRJ010000000
VERSION     DAZFRJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.399"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..314)
                     /locus_tag="ACHKUN_004966"
     CDS             complement(<1..314)
                     /locus_tag="ACHKUN_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663049.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSS"
     gene            complement(366..575)
                     /locus_tag="ACHKUN_004967"
     CDS             complement(366..575)
                     /locus_tag="ACHKUN_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6663050.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(671..1045)
                     /locus_tag="ACHKUN_004968"
     CDS             complement(671..1045)
                     /locus_tag="ACHKUN_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6663051.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(1051..1767)
                     /locus_tag="ACHKUN_004969"
     CDS             complement(1051..1767)
                     /locus_tag="ACHKUN_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6663052.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
BASE COUNT          399 a          573 c          434 g          412 t
ORIGIN      
        1 gatgactgcg acgcctgttt cagacgggca ttcacggaac tccactcatc agccagtgaa
       61 atcaggtgtc cggtggcaaa ggcaccggca aatgcgccag ccattccgac agcagaaccg
      121 cgaatttccg tcaactggct gttcagttct gccagggcac gtcgctgctc ccgggctgcc
      181 gcagcggcct gacgcccgcc attctgcagg gtccggtaat attcactgcc catacgggac
      241 gcccgctgga tctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca
      301 cgtaacgtcg ccattcacct ttctccgggc aaaaaaaacc tgccacagca ggttttcatc
      361 attatttatg acattgctgc aaggctcagc gcgtcttcca gcgccgcaaa cggatccacc
      421 tccggcttat cctcatcctc gccccagcag agcatggcgt ccttcagtgc aacattcatc
      481 ccctgtgccc cgaaaaccgc tttcacgatc tgtgcattac gaatatcccc gcgctcatca
      541 cccagcgggg ataccctgtc gaactccatc cacatcatcg cctcgctcgc actcaggctg
      601 tgccgcagtt cggataaggt gcgccccaga cggagcgcaa gtcgcatcag aaagcgaatt
      661 tccgggcggg ctactttttt ctggccgact ctgcatcagc gatcagttcc agtgcctgac
      721 gcagcaaccg ggcatgtacc ggaccataga cggccagcac ctgctcacgg tcgtccggag
      781 tgaacacccg ttgcaggtca gtatcacaca ggacatcgca gaacagcgtc acatccgctt
      841 ccaggttacg gcgggttttc gccaccaccg acagggtatc gtcatcctct ccatcaccat
      901 tgagcacttc ctgccacaga taccaggcct ctgccgaagg ctcccgcagc accacgctga
      961 catttctcca ttccggcacc ttcaccgttt tatgacggaa ccccgacagt ctggccagcg
     1021 ccagtgtttt cagatctttt gccataagcc ttatccgccc gcaccattaa ccgttactgt
     1081 acacgcatca gaggtaatgc tctgcggctg ttctgcagaa tccgttacct cgcaggtata
     1141 agccccctta tcacctgact gcgtattggc tttactgaaa gtgtcagtag tctgtccctc
     1201 taccggctga ccatccttct tccaggcgtg tttataaggc ggcgttcccc cgttgacact
     1261 gactgacatt gtcagcagcg caccggtatt cacggtaagt gtcttctcca gatttttcac
     1321 aaacgccagc ggtaccacat aggacaccgg tttacccttc aggcgaagtg aaaacgttgc
     1381 agccaccacg ccgttggtac cggatgacca ggtgtgctga cgcacttccg ccaggaactt
     1441 aaagccctta ccggacggaa actgcacctt aaacgcatac accgtgtcat tgtcataggc
     1501 atcacgcagg gcgttctggg cctgattcag ataaaaatta cccgacatgg aaatctcgga
     1561 cgacgccccc agaccgttga tgttctcctg ctctgtggag cagagcgtgg tcacatcaat
     1621 atcctgtttc tgaccggcgg tgaactggac ttccttgatg gtgcagtcca ggcgcagata
     1681 ttccgcctta tccatagttt cagcagtcgc cggggcagat gaaatcatca cctgcgtcag
     1741 ctgtgagcgt tcatacaaag cagacattct gcctcctgat aataaaaaac ccgcacgcgg
     1801 cggggtatgg gttttgta
//
LOCUS       DAZFRJ010000397         1807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000397 DAZFRJ010000000
VERSION     DAZFRJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.400"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..325
                     /gene="bssS"
                     /locus_tag="ACHKUN_004970"
     CDS             71..325
                     /gene="bssS"
                     /locus_tag="ACHKUN_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO6663053.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     KVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            440..1558
                     /gene="solA"
                     /locus_tag="ACHKUN_004971"
     CDS             440..1558
                     /gene="solA"
                     /locus_tag="ACHKUN_004971"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO6663054.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1606..1719
                     /locus_tag="ACHKUN_004972"
     CDS             1606..1719
                     /locus_tag="ACHKUN_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO6663055.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          463 a          435 c          437 g          472 t
ORIGIN      
        1 aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta
       61 gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga
      121 catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa
      181 taagtccgag ctggaaggga ctaaagttgg tcagacgctc tggttaacca ctgatgttgc
      241 cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc
      301 ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc
      361 ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca
      421 tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg
      481 cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca
      541 tatgccaccg catcaacacg gcagccacca tggcgatacg cgattaattc gccatgctta
      601 tggtgaaggc gaaaagtatg tcccgctggt cctccgcgcg caaacgctgt gggatgaact
      661 ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc
      721 ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt
      781 tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa
      841 ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac
      901 ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgt
      961 aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt atcaggcgaa
     1021 aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca
     1081 gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa
     1141 attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga
     1201 aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga
     1261 acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa
     1321 tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc
     1381 tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg
     1441 cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc
     1501 gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc
     1561 atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg
     1621 cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg
     1681 ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact
     1741 gacaaatatt taatctccct ttcatttggc gtgaaaaatt gaatgagatt attcctgctt
     1801 ctacagc
//
LOCUS       DAZFRJ010000398         1798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000398 DAZFRJ010000000
VERSION     DAZFRJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.401"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHKUN_004973"
     CDS             complement(<1..194)
                     /locus_tag="ACHKUN_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFO6663056.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVA"
     gene            complement(200..898)
                     /locus_tag="ACHKUN_004974"
     CDS             complement(200..898)
                     /locus_tag="ACHKUN_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6663057.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(898..1227)
                     /locus_tag="ACHKUN_004975"
     CDS             complement(898..1227)
                     /locus_tag="ACHKUN_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663058.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(1224..>1798)
                     /locus_tag="ACHKUN_004976"
     CDS             complement(1224..>1798)
                     /locus_tag="ACHKUN_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663059.1"
                     /translation="QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSA
                     IGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSR
                     IGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDGTNGQIGPAAL
                     KAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          374 a          598 c          440 g          386 t
ORIGIN      
        1 gccacaatct ccccctgcat ttctgcctgc agccagtctt ccggcgacat acggaaatac
       61 gcctccggct caccggagat attcacgcag gggaaatatc tttccccctc cggcgtgctt
      121 accacgaagc cgcacgactc cgctggcgca catcgccggg cgtgcgccag aatcgctgat
      181 tctgtctgtg tcatgggatt tactgcgaaa gtttgttaat ggaaaggaag ccgccaaagt
      241 tgccgacgtt attgcggaac ttacaaccgc tcaggcattt gctgcattta tccttcgtga
      301 tatcggacgt tggctggtca tattcatccg cgacagccgg accgctataa ccgcactcgt
      361 caccgcgata ggtccaggtg caggtgttgg ccagcatgat acgtcccgga aaaacagcgc
      421 catccgtttc cgtcggcgtg gacagtacaa aggaggcact caccgcgctc agttcgctgc
      481 actgctcgat gcgccagcgg ctgatcacct cctgctccgg atcggcgtca ctgtttccgt
      541 tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc accgttccgc
      601 cgaccagact ctgcagatct tccgccatcc cggtgaccat accgtacagg ttagaaaccg
      661 tcagcgtggg gcgcgtactg gtgcctttgc cattcagttc aaaaccgctc ccctgaatgg
      721 gatacggctg atactgtcgc ccctgccagg tgaccggctc acctttttcg ttctgctcat
      781 tacagaaaaa ataacgttca ccaccgacct ctgtcagatc gatttcccag agcaccacgc
      841 tggccgactg ctccgcacgg gtgcattcat tcagtgtttc ctgccggata tcctgcatca
      901 gttcaccacc tgtttaaact ctgcgctgaa ctcaacacgc aacatactga cctgcgacga
      961 ccattttgcg caggtcacct ttatctgcct gtaaccataa ggcggcgtcc acagaaaggc
     1021 cttccagccc ccgtgctcag ccagaaacga ctccagcgcc gtggcctcct cacgggagac
     1081 agacagcgtc acgctgtacg ttttcaggtc agcgttcagc ccggcaggca cacgctggga
     1141 atagccatca ccaaagcgca cctccctgac ggaaggggcc gatgtcacat ccataccggg
     1201 tttcactttc cagcggaagg ttttcatcgt ccacctccgg agaacaggcc accatcacgc
     1261 atctgtgtct gaatttcatc acgggcaccc ttgcgggcca tgtcatacac cgccttcaga
     1321 gcagccggac ctatctgccc gttcgtgccg tcgttgttaa tcaccacatg gttattctgc
     1381 tcaaacgtcc cggacgcctg cgaccggctg tctgccatgc tgcccggtgt accgacataa
     1441 ccgccggtgg catagccgcg catcagccgg taaagattcc ccacgccaat ccggctggtt
     1501 gcctccttcg tgaagacaaa ctcaccacgg tgaacaatcc ccgctggctc atatttgccg
     1561 ccggttcccg taaatcctcc ggttgcaaaa tggaatttcg ccgcagcggc ctgaatggct
     1621 gtaccgcctg acgcggatgc gccgccacca acagccccgc caatggcgct gccgatactc
     1681 ccgacaatcc ccaccattgc ctgcttaagc agaatttctg tcatcatgga cagcacggaa
     1741 cgggtgaagc tgcgccagtt ctgctcactg ccggtcagca tcgccgccat attctgtg
//
LOCUS       DAZFRJ010000399         1798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000399 DAZFRJ010000000
VERSION     DAZFRJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.402"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHKUN_004977"
     CDS             complement(<1..194)
                     /locus_tag="ACHKUN_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFO6663060.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVA"
     gene            complement(200..898)
                     /locus_tag="ACHKUN_004978"
     CDS             complement(200..898)
                     /locus_tag="ACHKUN_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6663061.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFHNNV
                     GNFGGFLSINKLSQ"
     gene            complement(898..1227)
                     /locus_tag="ACHKUN_004979"
     CDS             complement(898..1227)
                     /locus_tag="ACHKUN_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663062.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(1224..>1798)
                     /locus_tag="ACHKUN_004980"
     CDS             complement(1224..>1798)
                     /locus_tag="ACHKUN_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663063.1"
                     /translation="QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSA
                     IGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSR
                     IGVGNLYRLMRGYATGGYVGTPGSLADSRSQASGTFEQNNHVVINNDGTNGQIGPAAL
                     KAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          375 a          594 c          436 g          393 t
ORIGIN      
        1 gccacaatct ccccctgcat ttctgcctgc agccagtctt ccggcgacat acggaaatac
       61 gcctccggct caccggagat attcacgcag gggaaatatc tttccccctc cggcgtgctt
      121 accacgaagc cgcacgactc cgctggcgca catcgccggg cgtgcgccag aatcgctgat
      181 tctgtctgtg tcatgggatt tactgcgaaa gtttgttaat ggaaaggaag ccgccaaagt
      241 tgccgacgtt attgtggaac ttacaaccgc tcaggcattt gctgcattta tccttcgtga
      301 tatcggacgt tggctggtca tattcatccg cgacagccgg accgctataa ccgcactcgt
      361 caccgcgata ggtccaggtg caggtgttgg ccagcatgat acgtcccgga aaaacagcgc
      421 catccgtttc cgtcggcgtg gacagtacaa aggaggcact caccgcgctc agttcgctgc
      481 actgctcaat gcgccagcgg ctgatcacct cctgctccgg atcggcgtaa ctgtttccgt
      541 tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgcctgacc accgttccgc
      601 cgaccagact ctgcatatct tccaccatac cggtgaccat gccgtgcagg ttagaaaccg
      661 tcagtgtcgg acgggcagca ctgcccttgc cgttcagttc aaatcccgtc ccctgaatgg
      721 gatacgcctg atactgtcgc ccctgccagg tgaccggctc acctttttcg ttctgctcat
      781 tacagaaaaa ataacgttct ccaccgacct ctgtcaggtc gatttcccag agcaccacgc
      841 tggccgactg ctccgcacgg gtgcattcat tcagtgtttc ctgccggata tcctgcatca
      901 gttcaccacc tgttcaaact ctgcgctgaa ctcaacacgc agcatactga cccgcgacga
      961 ccattttgcg caggtcacct ttatctgccg ccactcataa ggcggcgtcc acagaaagga
     1021 tttccagccc ccgtgctctt ccagaaacga ctccagtacc gtggcctcct cacgggggac
     1081 agaaagcgtc acgctgtacg ttttcaggtt ggcattcagc ccggcaggcg ctcgctggga
     1141 atagccatca ccaaagcgca cctttcttac agaaggggcc gaagccacat ccataccggg
     1201 tttcactttc cagcggaagg ttttcatcgt ccacctccgg agaacaggcc accatcacgc
     1261 atctgtgtct gaatttcatc acgggcaccc ttgcgggcca tgtcatacac cgccttcaga
     1321 gcagccggac ctatctgccc gttcgtgccg tcgttgttaa tcaccacatg gttattctgc
     1381 tcaaacgtcc cggacgcctg cgaccggctg tcagccagac tgcccggtgt accgacataa
     1441 ccaccggtgg catagccgcg catcagccgg tagagattcc ccacgccaat ccggctggtt
     1501 gcctccttcg tgaagacaaa ttcaccacgg tgaacaatcc ccgctggctc atatttgccg
     1561 ccggttcccg taaatcctcc ggttgcaaaa tggaatttcg ccgcagcggc ctgaatggct
     1621 gtaccgcctg acgcggatgc gccgccacca acagccccgc caatggcgct gccgatactc
     1681 ccgacaatcc ccaccattgc ctgcttaagc agaatttctg tcatcatgga cagcacggaa
     1741 cgggtgaagc tgcgccagtt ctgctcactg ccggtcagca tcgctgccat attctgtg
//
LOCUS       DAZFRJ010000400         1775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000400 DAZFRJ010000000
VERSION     DAZFRJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.403"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(310..582)
                     /locus_tag="ACHKUN_004981"
     CDS             complement(310..582)
                     /locus_tag="ACHKUN_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663064.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            742..1275
                     /locus_tag="ACHKUN_004982"
     CDS             742..1275
                     /locus_tag="ACHKUN_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6663065.1"
                     /translation="MHEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1496..1609
                     /locus_tag="ACHKUN_004983"
     CDS             1496..1609
                     /locus_tag="ACHKUN_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663066.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1611..>1775
                     /locus_tag="ACHKUN_004984"
     CDS             1611..>1775
                     /locus_tag="ACHKUN_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6663067.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDHAIIYKEQRDKKASEL
                     ELANATITDMQ"
BASE COUNT          474 a          344 c          479 g          478 t
ORIGIN      
        1 aacctgtatt tcaaaatcag agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg
       61 aataaacaat acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag
      121 gtaagaaaag gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc
      181 gctttctctt tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac
      241 agctgtgcaa gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg
      301 attttatggc tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca
      361 tgcgtaacga gaatcccata agcagggagg acttaattct tcattaaccc atgcgttgat
      421 attatgtttc agccgttgaa gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt
      481 tttatgtgag atcttaccgt aagggtctgc aagaatgctg cttgttgctt cgttattatc
      541 tgccatcaga agaagtaact ctgatttaac gttttctgtc attagttgta aaaatcttct
      601 gcgcaaactt tctttactgt tcatttatat ggcttcattt gttgtaatct gctgcgtctc
      661 aagggatatg tttatgagag cgaccatgag tgttggatta tatacctaac atatcaaggg
      721 attagaaatc gataaatccc catgcacgaa aaaataaaat acggcctgtc ggctgccgtt
      781 ctggcgctga ttggtgcagg tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa
      841 aaggaaggta accacaccac ggcataccgt gatggtgcgg ggatctggac catctgccgt
      901 ggtgccattc tggtggatgg taagcctgtt attcctggca tgaagctgtc aaaggaaaaa
      961 tgcgaccggg ttaatgccat cgaacgtgac aaggcgctgg catgggtgga gaaaaacatc
     1021 cgggtgccgc tgaccgaacc ccagaaagcg gggatcgcgt cattctgtcc gtacaacatt
     1081 ggccccggta agtgcttccc gtcgacgttt tataaacgaa ttaatgcagg cgatcgaaaa
     1141 ggtgcctgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
     1201 tcaaataatt gctacggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
     1261 gacatcgaca gatagcagaa tattttcctg aaaaatgacg ttggccaacg cgggtggata
     1321 acacgaaatc ctgaaaactg gtaaaaccta agtgaataaa agtaaaaacc ccgtttgttg
     1381 gcagcaagcg gggttttgtg ttttctgacc ttgagcaagg caagggagaa attatgggta
     1441 gggaggtact ttccctgtga ggaagtataa aagattcttt ctgaggttgt ccattatgaa
     1501 aggcattgaa gtggagacgc cagccagtct ggatttaaca agagcggcag cttttgccat
     1561 tcgtattgtg gccattgctg ttctggtctg ggcaatccgt tggtggtgat atgaaccgtg
     1621 ttctgtgcgt ggttatcatt gtcctgctgg tggcctgtgg tgcgcttagt ctggggctga
     1681 atcattaccg tgatcacgcc atcatctaca aagagcagcg cgataaaaaa gccagtgagc
     1741 tggagctggc gaacgcgaca attactgata tgcag
//
LOCUS       DAZFRJ010000401         1767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000401 DAZFRJ010000000
VERSION     DAZFRJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.404"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..577)
                     /gene="nleH1-2"
                     /locus_tag="ACHKUN_004985"
     CDS             complement(<1..577)
                     /gene="nleH1-2"
                     /locus_tag="ACHKUN_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO6663068.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYG"
     gene            complement(684..755)
                     /locus_tag="ACHKUN_004986"
                     /pseudo
     CDS             complement(684..755)
                     /locus_tag="ACHKUN_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            749..1743
                     /locus_tag="ACHKUN_004987"
                     /pseudo
     CDS             749..1743
                     /locus_tag="ACHKUN_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          532 a          424 c          380 g          431 t
ORIGIN      
        1 aaccataata ctgattgaaa caacgaactt cgcttgtcac ctcttcatgg ctttgagata
       61 tagtaaacat cttcaacact tttgttgtat cttccatatc ttcatacacg acagcattac
      121 cacctttacc aataacatta cctggcacgg gtgatttatt gttgctaacc ctcggcaact
      181 ctgattgagt ataatccgct ggaggtaact ccggctgcgc ataatccact ggaggtaaat
      241 caggtctgtt cgaatgaaca gagcctcttt ctatagtcgt gctcactggc gatgtattca
      301 gcatagcctc aatttttctg ctaatctctc ccttaggcca tcccaacctg tgcaacagat
      361 tagtaaaaca accactatga ctttctcttg taacgcaaaa cttattatca gtgaccacaa
      421 cacgatatgt tctgttgcca accgttactt gcgtcccgct atcagagtga acagcagcat
      481 cccttacaga ggataaaaca cgattatcag gcgaagtcag gtttctggtt aaagaattcc
      541 atgaacatcc caaatttata gaagagggcg ataacataca tttcaacctt caaaataaac
      601 ctatctaatt attcctaaca gacatccccc atacatgaca acaaaaaccg gagccggact
      661 ccggtttttg tgaagctatc gggttacttc atttcgccaa tattttccca cttcccgtca
      721 gcacgcagga tttgcagcgg tcttactaca cactgacccg aggacgaaaa aagagttcgg
      781 gctgggaaga gacagaagag tagcaatttc agaagcattc acaccaacat tgagcttttc
      841 agcgaaagtg ggcacgaatc attgctggac agggttaaag gcactgactc aatcactctt
      901 catacgtggc ttgatcgata tgaaacaatc ctcagctaga gggggagcaa gccgaaaaca
      961 ttactcgact acgccagcaa aatcagggca attcgaagaa aattgccgga caaaccgctc
     1021 actgacatat caacgaaaga ggtggcagca atgctaaaca cctacgtcgc agaaggtaaa
     1081 gcggtttccg caagagtaat caggtcaacc cttgttgacg tttttcgagg ggcaatagcc
     1141 gaggggcatg tggcaacgaa tccagtaaca acaacccgtg cagcaaagtc agaagtaagg
     1201 cgctcaaggc tgacagctaa tgagtatgtc gctatttacc atgctgctga gcacctcccc
     1261 atctggctga ggctgtcaat ggatttagct gtcgttacag ggcagagagt gggcgatttg
     1321 tgcagaatga agtggtcaga cataaacgat ggtcatcttc acattggaca gagtaaaaca
     1381 ggagccaaaa ttgccattcc gctggctcta accattgacg cactcgacat ctcactggtt
     1441 gatacactac agaaatgcag ggaggccagc agcagtgaaa caataatcgc atcaacctat
     1501 cacgaaccac tttctccagc cacagtatca cggtatttaa caaaggcgcg aaatgcatcc
     1561 gggatctcgt ttgatggaga cccaccgaca tttcatgaac tacgtagtct gtccgcgagg
     1621 ctatatcgga accagattgg ctacaagttt gcacaacgtc ttcttggaca taaatctgat
     1681 tcaatggcgg cgcattatag ggacagtcgc gggcgggagt gggacaaaat tgaaatcgga
     1741 taatgatttt attttgacca ataatga
//
LOCUS       DAZFRJ010000402         1744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000402 DAZFRJ010000000
VERSION     DAZFRJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.405"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..382
                     /locus_tag="ACHKUN_004988"
     CDS             38..382
                     /locus_tag="ACHKUN_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO6663069.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            433..966
                     /locus_tag="ACHKUN_004989"
     CDS             433..966
                     /locus_tag="ACHKUN_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6663070.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1237..>1744
                     /locus_tag="ACHKUN_004990"
     CDS             1237..>1744
                     /locus_tag="ACHKUN_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO6663071.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEA"
BASE COUNT          483 a          373 c          480 g          408 t
ORIGIN      
        1 gaggaccgcc gtaagacggc gcggggagac taaagcgatg aagaaaaaat acgaactggt
       61 tgttaaagag ataaataatt acccggataa gattgctgtt actgtggcac ttgaaattgg
      121 cgggcatccg tcgttgttgt tgccacatgt ggcgattagt cttgaccgta ctgaaggtgc
      181 cacgctggag ttttacgaag ctgaggcgaa aaagcaggcg aagcaatttt tcatggatgt
      241 tgctgccggg ttatgtgaag gggatggtcc gttaccggaa aagcgtcccg taattttaga
      301 ggcgcaggat gtgttgataa cctacagagg aaaactaccg ggaataatta cgggttctct
      361 gaagactcca ccgctggcct gaagacttaa catatccagg gatttgaaat cgataaaccc
      421 tgataaatat ccatgaacgc aaaaatcaga tacggcctgt cggctgccgt tctggcgctg
      481 attgccgcag gtgcgcctgc gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt
      541 aaccacacca cagcataccg tgatggtgcg ggtatctgga ccatctgccg tggagccacc
      601 cgggtggatg gtaagcctgt tattcctggc atgaagctgt cgaaggaaaa atgcgaccgg
      661 gttaacgcca ttgagcgtga taaggcgctg gcatgggtgg agaaaaacat cagagtgcca
      721 ctgaccgaac cccagaaagc ggggattgcg tcattctgtc cgtacaacat tggccccggt
      781 aagtgtttcc cgtcgacgtt ttacagacgg attaatgcag gagatcgaaa aggtgcctgc
      841 gaagctattc gctggtggat taaggacggt ggcagggact gccgtattcg ctcaaataac
      901 tgttacggtc aggtatcccg tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac
      961 agataagcag aatattttgc tgaaaaatga ggtttgctta catggatgga taacacgaaa
     1021 tcctgcaaat tggcaaaatg taagtgaata aagtcaaaac agttgtttaa cactcaggca
     1081 ccgtaatgat gcctttgtca tttctgcgca tctcacgcgc atctcacaac acagaacctt
     1141 tcaggatgac ccttgaggat accggtttgg ctgtcggtgc ctttctgtgg gctggattcc
     1201 tgtgagacaa ggttcatcac taaaaggaaa taaccgatga atatgatggc cgtgccgttt
     1261 cacggcaact ctctttatgt agttaaccat aatggcgaac catacgttcc catgaaacct
     1321 gtcgttgcgg ggatggggct ggcctggcaa tcacagttgg ctaagttaag acagcgtttt
     1381 gcgtcaacta taacggaaat cgttatggtt gctgaggatg ggaaacaacg caatatggtg
     1441 tccatgccac ttcgaaaact tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa
     1501 cccgaaatcc gcgataaggt catccggtat caggaagagt gcgacgatgt tctttacgag
     1561 tactggacga agggttttgt cgttaatccc cgtaaaatga gcgtgatgga agaactcaac
     1621 caggcttgtg ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg
     1681 aatgagtgga aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa
     1741 gcga
//
LOCUS       DAZFRJ010000403         1717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000403 DAZFRJ010000000
VERSION     DAZFRJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.406"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..409)
                     /locus_tag="ACHKUN_004991"
     CDS             complement(<1..409)
                     /locus_tag="ACHKUN_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFO6663072.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQ"
     gene            complement(422..844)
                     /locus_tag="ACHKUN_004992"
     CDS             complement(422..844)
                     /locus_tag="ACHKUN_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6663073.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKTQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(841..1095)
                     /locus_tag="ACHKUN_004993"
     CDS             complement(841..1095)
                     /locus_tag="ACHKUN_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6663074.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADVILTIGRNKTTTKKLMGVDS"
     gene            1175..1594
                     /locus_tag="ACHKUN_004994"
     CDS             1175..1594
                     /locus_tag="ACHKUN_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6663075.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
BASE COUNT          477 a          401 c          355 g          484 t
ORIGIN      
        1 cttgttttgt ttgaacatct gcttcctttc tgcttcttct ggcctgaaca gatgcttttc
       61 ctgcggctga ttttttggtt aatttttccc tgactgatgc cagatcttcc tcaatccgaa
      121 gatgcaccca ttcctcgccg ttatcgcaaa aaaactcctg caaggatggt tcaacatcag
      181 cccatcgctc gttagtcaga cgggcaattt ttgccagcct gtttttaggt attggctttc
      241 ctgtttgcca gtaattgaac atcagcaaca aatacgcacc atgctcctct gctgacaaat
      301 gcatggtgtc agccaggtaa tcagctatgt acagttgcat gtatggtaat gcggccataa
      361 ttgccccgta tgatgctgcc cggtggctta gaataagcac aaacagcatg gaaacttttg
      421 cttaatgaac aatgacagaa tcgtcggaag acccgccgcc gctgaaatgc gctttccggt
      481 aaacggcttg gactgcatca tcatgcgcat caattgccgt acttaacgct tcctgcgccg
      541 ccagtaatgc acggcgttcc agggtatcga agatgcagag tcggtgacgc agctcgcgcg
      601 gaaggattgc cagaattgct gggatcagct tctgaatttt ttctctttgc gttttcgttt
      661 cacctttcaa ccaacggtga tagatattct gctgattatt ccagtccttg cctggtacca
      721 ggggcaattc gccgcccccc tggcgcagat attcttcagt aatggcattg gctacccatg
      781 cctgcccttt ttcggctgcc agggctaaca acactgactc gatgtgctca tgcttgattt
      841 tcatgaatca actcccatca gctttttcgt agtagtttta tttctgccaa tagttaaaat
      901 tacatcagca gaaaataatc cgtttgatgc atgagcgatt ttttcagcgt aatttgtttc
      961 gccggtatat tctgtgcgag gcaattttcc gttatccatc catttgtaga ttgctctttg
     1021 gctgacacca caaacgtcgg ccacaacaga aacgcgaaca gttttgatta catcttcaag
     1081 tgttttctgg ttcatatcat cctcacaatg tgaactttga gtacatgcta taacagaact
     1141 gacagtacat tcaagagcga atatcattga acttatggtt catgaagata aagcgcgtaa
     1201 agagttcgcc agtaggcttg cgctagcctg tgaaaacgct ggttatgaac aacatggaag
     1261 gcaggcagaa attgcccgtc gaatgaaatt aacaccaaaa gcggttagca aatggtttaa
     1321 tggcgaaaca attcctcgcc gagagaaatt aagggaatta gcaacactca ttggaacaac
     1381 accaacctat cttttgggag aggatacaga agaaagtgga caggtacgtt tctatcagga
     1441 gttaaatcca agacaaaaaa tcatcattga ccttctggac gagctccctg acagtgagac
     1501 agatgaactt ttaaaaactc ttgaggagaa aaaacagaag tacaatgcaa tttacgaaga
     1561 gctagcacga aagaaaaaac aaaaagcctc ttaaaccagc ataaatccgg tagcgtcccc
     1621 ctccgggttt gtgcttcact ttatcccatc tcattttttt acacacaaaa tgtacttaaa
     1681 gtactttaca tcaatgaaca caaagtacat tatatac
//
LOCUS       DAZFRJ010000404         1702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000404 DAZFRJ010000000
VERSION     DAZFRJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.407"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            215..394
                     /gene="yciG"
                     /locus_tag="ACHKUN_004995"
     CDS             215..394
                     /gene="yciG"
                     /locus_tag="ACHKUN_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6663076.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            480..980
                     /gene="yciF"
                     /locus_tag="ACHKUN_004996"
     CDS             480..980
                     /gene="yciF"
                     /locus_tag="ACHKUN_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6663077.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            1026..1532
                     /gene="yciE"
                     /locus_tag="ACHKUN_004997"
     CDS             1026..1532
                     /gene="yciE"
                     /locus_tag="ACHKUN_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6663078.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1592..>1702)
                     /locus_tag="ACHKUN_004998"
     CDS             complement(1592..>1702)
                     /locus_tag="ACHKUN_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO6663079.1"
                     /translation="YMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          559 a          346 c          361 g          436 t
ORIGIN      
        1 ccgcaacata acaccagtaa aatcaataat tttctctaag tcacttattc ctcaggtaat
       61 tgttaatata tccagaatgt tcctcaaaat atattttccc tctatcttct cgttgcgctt
      121 aatttgacta attctcatta gcgactaatt ttaatgagtg tcgacacaca acactcatat
      181 taatgaaaca atgcaacgca acgggagaaa taacatggcc gaacatcgtg gtggttcagg
      241 aaatttcgcc gaagaccgtg agaaggcatc cgacgcaggc cgtaaaggcg gtcagcatag
      301 cggcggtaat tttaaaaatg atccgcaacg cgcatctgaa gcgggtaaaa aaggcggtca
      361 acaaagcggt ggtaataaat caggcaaatc ctgattcacc gttaattatt aatatcagct
      421 gataaagata attgcatctg cgggccgtat ggctcgcagt gatttcactg gagaaaaata
      481 tgaatatgaa gaccattgaa gatgtattta ttcacctgct ttcagatacc tacagcgcag
      541 aaaaacaatt aacccgggca ctggcaaaac tcgcaagagc aacatcaaat gaaaaattaa
      601 gtcaggcttt tcatgcgcac ctcgaggaaa ctcatggaca gattgaacgt attgatcaag
      661 ttgtggagtc cgaatcgaac ctgaaaatta agcgcatgaa atgtgtggca atggaaggtc
      721 ttattgaaga agctaatgag gtcatcgaaa gtaccgagaa aaacgaagtg cgtgatgccg
      781 cactgattgc cgcagcgcag aaagtcgagc attatgagat tgccagttac gggacattag
      841 cgacgctggc tgaacaatta ggttaccgta aagcagcgaa gcttctgaaa gaaaccctgg
      901 aagaagaaaa ggccaccgac atcaaactga ctgatctggc ccttaataac gtaaataaga
      961 aagccgaaaa taaagcctga aatatgaatt ttaactttta gtcattttat aaagaggaca
     1021 ttttcatgaa tcgtattgaa cattatcatg actggttacg tgacgcccac gcaatggaaa
     1081 agcaagccga atctatgctt gaatccatgg ccagccgtat agataattat cctgaactac
     1141 gcgctcgtat tgaacaacat cttagtgaaa ccaaaaacca gattgttcaa ctggaaacta
     1201 ttcttgatcg taatgacatt tcacgttcag tcattaaaga ttccatgagt aaaatggctg
     1261 cgcttgggca gtcaatcggt ggtatattcc cttctgatga aatagttaaa ggctctatta
     1321 gcggatatgt cttcgagcaa tttgaaatcg cctgttacac ctcactatta gcagcagcaa
     1381 aaaatgccgg tgatacagtc tcaattccga tcatcgaagc gattttaaat gaggaaaagc
     1441 aaatggccga ctggctgatt cagcatattc cgcaaacaac tgagaaattt ttaattcgtt
     1501 ctgaaactga tggcgtagaa gcgaagaaat aatacccttt ataccatgtc cttattgacc
     1561 ccgtatatta cggggtcgtt tttgtgcgga attaaaaacg atatcctgct gagaacataa
     1621 acacccacgg atcgaggcgt acgctatcgt gttgctgtgc accgcctagc ttataattgg
     1681 cggtggtatc gatatccatg ta
//
LOCUS       DAZFRJ010000405         1696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000405 DAZFRJ010000000
VERSION     DAZFRJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.408"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..728
                     /gene="hchA"
                     /locus_tag="ACHKUN_004999"
     CDS             <1..728
                     /gene="hchA"
                     /locus_tag="ACHKUN_004999"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO6663080.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
     gene            complement(836..>1696)
                     /gene="hprS"
                     /locus_tag="ACHKUN_005000"
     CDS             complement(836..>1696)
                     /gene="hprS"
                     /locus_tag="ACHKUN_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6663081.1"
                     /translation="VAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSA
                     LPRELKPLGQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSI
                     AEYQKTIAGNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYL
                     SDEKEICFKVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNI
                     DIASPGTKINESEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHN
                     VFRITLPQRN"
BASE COUNT          463 a          393 c          363 g          477 t
ORIGIN      
        1 caaaaccgta tcgcggtaaa cataaaattc tggtgatcgc cgcggacgaa cgttatttgc
       61 cgaccgataa cggaaaactg ttctcgaccg gtaaccatcc gattgaaacg ttgctgccgt
      121 tgtatcatct ccatgctgca ggtttcgaat tcgaagtggc gaccatttcc ggtctgatga
      181 ccaagtttga atactgggct atgccgcaca aagatgaaaa agtaatgcca ttctttgagc
      241 agcataaatc gttgttccgc aatccgaaaa aaatcgcgga tgttgtcgtc agcctcaacg
      301 cagacagcga atatgcagca atctttgttc ctggtggtca tggcgcactt attggtttac
      361 cagaaagcca ggacgtggct gctgctttgc agtgggcaat caaaaatgac cgttttgtta
      421 tctccctttg ccacggcccg gcggcttttc tggcgcttcg ccacggcgat aacccactga
      481 atggttattc catttgcgca ttcccagacg ccgcagacaa acaaacgcca gagataggct
      541 atatgccggg tcatctcacc tggtacttcg gcgaagagct gaagaaaatg ggcatgaaca
      601 ttattaatga cgacatcacc gggcgagtac ataaggaccg taaagttctc accggcgaca
      661 ctcctttcgc agcgaatgcg ttgggtaaac tggcggcgca ggaaatgctg gcagcttatg
      721 cgggttaatc cctgttacgt atgaatgtaa gcgcataatc aatcgcagcc acgttaatac
      781 tcacgacgtg gctgcgtttt ttcatggttg atttatggcg tactgaagcc ctatgttaat
      841 ttctttgcgg taacgtaatt cggaacacat tatgcttatt aaaatagtga tacgtagcac
      901 ttcccccatg caattcggca atcgctttga ctaaagaaag accaagtccc tgaccttcgg
      961 aatgacgcga attatctccc cgccaaaatc tacggaagag tttttcagac tcattaattt
     1021 tcgttccagg actggcgata tcaatattaa gatagccgtt ggtatcaaga aaactggtta
     1081 tatgaatgcg cgatttttct ggtgaatatc taatggcatt aacaataaga ttcgataaca
     1141 ttcgttgcag taaaatttta tccgcaaaga tttgctgatt gcactcgacc ttaaagcaaa
     1201 tctctttctc gtctgaaagg tattcaagat aatccaacaa attttcgact tccttattga
     1261 gaaaaagtga gtccagtttc accaaaacat tgtttttatc tgcccgggca agaaacagta
     1321 tgttctctgt taaccgcgaa atattttcca gctcttcaat gttaccggca attgtttttt
     1381 gatattcagc gatacttctg gtttgactga gcgtaacctg attctgaccc agtaatgcat
     1441 taatgggcgt tctaagttca tgagcgagat cgtcagcaaa ctgacttaga cgctcaaaat
     1501 ctttgactaa agcatgatgc attttattca acgcctgccc aagaggtttt agttctctcg
     1561 gtaatgcgct aacctcaaca ggctcccggc tatcgttata attcagcgct tccgttacac
     1621 cactcaactt tttgatctct cgtaatcccg ttctgattaa cagcggactt aatactgagc
     1681 aaagtacaat ggcgac
//
LOCUS       DAZFRJ010000406         1693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000406 DAZFRJ010000000
VERSION     DAZFRJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.409"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1230
                     /locus_tag="ACHKUN_005001"
     CDS             <1..1230
                     /locus_tag="ACHKUN_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6663082.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..>1693
                     /gene="ybfC"
                     /locus_tag="ACHKUN_005002"
     CDS             1227..>1693
                     /gene="ybfC"
                     /locus_tag="ACHKUN_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO6663083.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDE"
BASE COUNT          488 a          382 c          451 g          372 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccgcc tgaccacaat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca cgccactcat cagggttgaa accgccaccg gtgagcaggc gaaaacgcag
      241 cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1681 ttaaagatga gaa
//
LOCUS       DAZFRJ010000407         1674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000407 DAZFRJ010000000
VERSION     DAZFRJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.410"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..159
                     /locus_tag="ACHKUN_005003"
     CDS             52..159
                     /locus_tag="ACHKUN_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663084.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            365..1648
                     /gene="gltA"
                     /locus_tag="ACHKUN_005004"
     CDS             365..1648
                     /gene="gltA"
                     /locus_tag="ACHKUN_005004"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO6663085.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          437 a          404 c          394 g          439 t
ORIGIN      
        1 ttgacaatca ttcaacaaag ttgttacaaa cattaccagg aaaagcatat aatgcgtaaa
       61 agttatgaag tcggtatttc acctaagatt aacttatgta acagtgtgga agtattgacc
      121 aattcattcg ggacagttat tagtggtaga caagtttaat aattcggatt gctaagtact
      181 tgattcgcca tttattcgtc atcaatggat cctttacctg caagcgccca gagctctgta
      241 cccaggtttt cccctctttc acagagcggc gagccaaata acaaacgggt aaagccaggt
      301 tgatgtgcga aggcaaattt aagttccggc agtcttacgt aataaggcgc taaggagacc
      361 ttaaatggct gatacaaaag caaaactcac cctcaacggg gacacagctg ttgaactgga
      421 tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc cgtactctcg gttcaaaagg
      481 tgtgttcacc tttgacccag gcttcacttc aaccgcatcc tgcgaatcta aaattacttt
      541 tattgatggt gatgaaggta ttttgctgca ccgcggtttc ccgatcgatc agctggcgac
      601 cgattctaac tacctggaag tttgttacat cctgctgaat ggtgaaaaac cgactcagga
      661 acagtatgac gaatttaaaa ctacggtgac ccgtcatacc atgatccacg agcagattac
      721 ccgtctgttc cacgctttcc gtcgcgactc acatccaatg gcagtcatgt gtggtattac
      781 cggcgcgctg gcggcgttct atcacgactc gctggatgtt aacaatcctc gtcatcgtga
      841 aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg gccgcgatgt gttacaagta
      901 ttccattggt cagccatttg tttatccgcg caacgatctc tcctatgccg gtaacttcct
      961 gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt aatccgattc tggaacgtgc
     1021 tatggaccgt attctgatcc tgcacgctga ccatgaacag aacgcctcta cctccaccgt
     1081 gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt atcgcagcag gtattgcttc
     1141 actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg ctgaaaatgc tggaagaaat
     1201 tagctccgtt aaacacattc cggaatttgt tcgtcgtgcg aaagataaaa atgattcttt
     1261 ccgcctgatg ggcttcggtc accgcgtgta caaaaattac gacccgcgcg ccaccgtaat
     1321 gcgtgaaacc tgccatgaag ttctgaaaga gctgggcacc aaagatgacc tgctggaagt
     1381 ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac tttatcgaga agaaactgta
     1441 cccgaacgtc gatttctact ctggtatcat cctgaaagcg atgggtattc cgtcttccat
     1501 gttcaccgtc attttcgcaa tggcacgtac cgttggctgg atcgcccact ggagcgaaat
     1561 gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg tatacaggat atgaaaaacg
     1621 cgactttaaa agcgatatca agcgttaatg gttgattgct aagttgtaaa tatt
//
LOCUS       DAZFRJ010000408         1671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000408 DAZFRJ010000000
VERSION     DAZFRJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.411"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..1223
                     /locus_tag="ACHKUN_005005"
     CDS             54..1223
                     /locus_tag="ACHKUN_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6663086.1"
                     /translation="MFQKNKIRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISNGRDRRLTSGEERRISRYFRDKNPQLYVIFHLALETAMRQGEI
                     LSLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLR
                     AYHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISH
                     ASVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            1265..1555
                     /locus_tag="ACHKUN_005006"
     CDS             1265..1555
                     /locus_tag="ACHKUN_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663087.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT          461 a          446 c          352 g          412 t
ORIGIN      
        1 gcatttctgt atccagcctc ctaccttccc cgtttttatc gtggaaggca gatatgtttc
       61 aaaaaaacaa aattcgcaaa atgtccctca gccgggcact ggacaaatac ctgaagactg
      121 tttccgtcca taaaaaaggt catctgcagg agttttatcg cgtcaatgtg attaaacgtc
      181 accctatggc cgatcgctat atggatgaaa tcactaccat tgatatcgcc ggttaccggg
      241 atcagcgttt agctcaaatt aacccacgaa cagggcgtca aatcaccgga aataccgtcc
      301 gtcttgaact tgccctttta tcatcattat tcaatatcgc ccgagtggaa tggggaacat
      361 gccgtatgaa tcctgttgaa ctggtccgaa aaccgaaaat cagtaatggc cgcgaccgcc
      421 gactgacttc tggtgaagaa cgccgcattt cccgctattt cagggacaaa aatccgcagc
      481 tctacgttat cttccatctg gctctggaaa cagcaatgcg ccagggagaa attctctcat
      541 tacgctggga acacctcgat ttgcagcacg gcgtggcgca tcttcctgaa acgaaaaatg
      601 gctcaccacg cgacgtacca ctctcccgga aagcacgtaa ctacctgcaa attctgccac
      661 aacagataaa cggcaatgtg ttcagttaca cctcgtccgg tttcaaaagc gcctggcgta
      721 ccgctcttct ggatctgaaa atagaaaatc tgcattttca cgatctccgc catgaggcca
      781 tcagccgtct gttcgagctc ggcacactga acgttatgga gattgcagcc atttccggtc
      841 accgctccct gaacatgttg aaacggtaca cacatctccg ggcatatcac ctggtcagta
      901 aactcgatgc aaggcgtaaa cagaccagca aaatttcccc ttattttgtt ccctaccctg
      961 caactgtcag aaacagaaac ggagtgttca ttgtgacact gcacgatttc gaccttgaaa
     1021 gccgtgctga aacccgggaa ctggcaatat ctcacgccag cgtattgttg ctgcgcgcac
     1081 tggcccaggc tgctcagcgg ggtgaacgcg tccccacacc aggcgagcta cccgcaaaca
     1141 ttgatgaacg ggtcatgatt tgtccgttaa caaacagcca gttcatgaca aaagcgaaca
     1201 gtattttcat atcctcctcc tgaacaaata atttttgttc aaaattaacg ctatgagacc
     1261 ctttatgaaa cacttatcct cccgtgcagc cactgcggat aataatcgta tcgttacaat
     1321 tgaagacgtt tacacaatcg cattctcgca accaattcac accgaatcag cgttggggca
     1381 aaggtggatg ttgatccata ctggtgaata tgaaagtgcc agcgatgcaa aacgcttcct
     1441 tgatacgctc gtcaccgggc actgtgcttg catggtaaca ccatcacctg ttctggatca
     1501 tgacacctgg ctgcgtatcc gcctgctgat gggcgagata agggatgcca aataaacatt
     1561 catggcccaa cgcataaaaa taacgccggg gactctggca gtggtaccat tctttttaca
     1621 tagagccaat atataaacaa cttttctatg ataatatctt tatcatgtta t
//
LOCUS       DAZFRJ010000409         1660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000409 DAZFRJ010000000
VERSION     DAZFRJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.412"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1412)
                     /locus_tag="ACHKUN_005007"
     CDS             complement(<1..1412)
                     /locus_tag="ACHKUN_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6663088.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEA"
     gene            1389..1531
                     /locus_tag="ACHKUN_005008"
                     /pseudo
     CDS             1389..1531
                     /locus_tag="ACHKUN_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1577..>1660
                     /locus_tag="ACHKUN_005009"
     CDS             1577..>1660
                     /locus_tag="ACHKUN_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663089.1"
                     /translation="MAKHGAGCLPVNSVSAPESAIIPYTWLL"
BASE COUNT          355 a          505 c          408 g          392 t
ORIGIN      
        1 gcctccttgg tcagcttcac tgccttacct ccggttccct ccgcatcgct gaccacttct
       61 gccctgccgc cactggctga ccatccctgt actttcaggc ttccctctga ctcgctggca
      121 aggtaagaga gcagtgttgt gacgcctgtg gcttctgcac cggaaggcga tgaagggcgc
      181 acctctgata ctgtcgatga tgcccccgcg tttagcgcca ctcttcccgc atggcgcaaa
      241 atcgccgttg ccagacggtc ggaaataatc ccacggcgtg cccaggcgct gaaatggctc
      301 gccctgtcct gtgacgtcca ggtggctgag ctgtcacgcc atttcgaacc gtaatatccg
      361 atacccggaa tgtccgggtc ttcttccggt ttgttcgtcg gcacattcac cccgttctca
      421 tcggtcatga acggtacgaa atggatattc ttttccgttt tgtttttata gctgccgtac
      481 accgtctggt acgtggattc gttcttctgc ttccagaaat acgtcgtatc tccacatatc
      541 cagggaacac cgccagcaga gccaccgacg cactgacctg ccatatccgc caggtctgca
      601 cggaatttat caaccagcgc accaaactgt gctgcgtgat ttgccggcgt accgccaaaa
      661 tcaaattccc cctgcatcca caccacggca aacagcacat ttttcggatt cttcttcagt
      721 gctgcttttg ttcgaccgat aaggtcctta tacagcggct tgtccacacc ccagcgggtt
      781 gaattctccg aggcaccact cgcgtcactg tatgtgccat cggctccggt ggtgaacgct
      841 gaaccaccac gacagcacgg aaccagcaga atgcccgcat tcgccggtat aaacggcagc
      901 aattttttgg cgatatgcag cccttgcccc acggttccgt actgtccctt tgacaggtcc
      961 gctttcggat ggttaaggcg gctcatgtcc tgcacatcat gcagacaatg gtccgccgga
     1021 atgatgtcgt tatatttaca ggcgacaccg cccggtgtca ccgtactgcg acgcgccagc
     1081 tgcttaatgc gcgggtccgg acggtcatat gtctccggca gcggaaggcc ttcaccatat
     1141 gccatgctgt ttgactgccc cgccagaacc acaacaaagt aatactccgg gtctctggtg
     1201 gcgctgatta ctgtgccttc tccatccgac ggcttcacca acacaggtgt ggtgacatca
     1261 ccttccgccg caatggcctg catcagagta taaggcgtga tggccaccgg gctgccaaat
     1321 ggctgccacc cctccttcag tttttgtgtc agcttttccg caaggtctga cggcgacgcc
     1381 gccctgacaa catcataatg tttaatcgac atcgaatttc tcccgtgtag aggaacagag
     1441 ttaaaaagcc ggaagcggaa tcaaatcaca ggatggccat ctgccagtgg ctggtcgtaa
     1501 aaaaaggcca cgccatgcgc agccggaaat aaagggataa cgatgatagt ttgagaaaaa
     1561 cagaaacaac acttttgtgg caaagcatgg tgccgggtgc ctcccggtga attcagtatc
     1621 agcacctgaa tccgcgatta tcccatatac ctggttgctg
//
LOCUS       DAZFRJ010000410         1656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000410 DAZFRJ010000000
VERSION     DAZFRJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.413"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..474)
                     /locus_tag="ACHKUN_005010"
     CDS             complement(31..474)
                     /locus_tag="ACHKUN_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6663090.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            631..1560
                     /gene="metA"
                     /locus_tag="ACHKUN_005011"
     CDS             631..1560
                     /gene="metA"
                     /locus_tag="ACHKUN_005011"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO6663091.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          401 a          464 c          369 g          422 t
ORIGIN      
        1 tccagcctgg gcaggcacgg caggggcggc ttattccccc acatacgcca gattcagcaa
       61 cggatacggt ttccccaaat cgtccacctc agagcgtccc gtaaccttaa aacccacctt
      121 cttatagaac ccaaccgcct gctcattttg ctcattaacg ttggttgtca gttccggtgc
      181 catcgagagc gcatgctcca ccagcatccg acccacgccg cagccgcgca catcaggatc
      241 gataaacagc gcatccatat gctgcccact taacaacata aacccaaccg gctgatcccg
      301 ttcattaacc gcgacccaca acggtgcttc cggcagaaag gaacgaacca gctcttccag
      361 ctcggcccga tactctgctg ataaaaaatc gtgggtggca tcgacagatc gacaccaaat
      421 cgcaacgagt tcctcccctt cctcatgctg tgagcggcga atactaataa ccattttctc
      481 tccttttcgt cattcttata ttctaacgta gccttttcct tgaaactttc tcaccttcaa
      541 catgcaggct cgacattggc aaattttctg gttatcttca gctatctgga tgtctaaacg
      601 tataagcgta tgtagtgagg taatcaggtt atgcctattc gtgtgccgga cgagctaccc
      661 gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga caacttctcg tgcgtctggt
      721 caggaaattc gtccacttaa ggttctaatc cttaacctga tgccgaagaa gattgaaact
      781 gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc aggtcgatat tcagctgttg
      841 cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc atctgaacaa cttctactgt
      901 aactttgaag atattcagga acagaacttt gacggtctga ttgtaaccgg tgcgccgctg
      961 ggcctggtgg agtttaatga tgtcgcttac tggccgcaga tcaaacaggt gctggagtgg
     1021 tcgaaagatc acgtcacctc gacgctgttt gtctgctggg cggtacaggc cgcgctcaat
     1081 atcctctacg gcattcctaa gcaaactcgc accgacaaac tctctggcgt ttacgagcat
     1141 catattctcc atcctcatgc gcttctgacg cgtggctttg atgattcatt cctggcacca
     1201 cattcgcgct atgctgactt tccggcagcg ttgattcgtg attacaccga tctggaaatt
     1261 ctggcagaga cggaagaagg ggatgcatat ctgtttgcca gcaaagataa gcgcattgcc
     1321 tttgtgacgg gccatcccga atatgatgcg caaacgctgg cgcaggaata tttccgcgat
     1381 gtggaagccg gactaggccc ggaagtaccg tataactatt tcccgcacaa tgatccgcaa
     1441 aatacaccgc gagcgagctg gcgtagtcac ggtaatttgc tgtttaccaa ctggctcaac
     1501 tattacgtct atcagatcac gccatacgat ctacggcaca tgaatccaac gctggattaa
     1561 tcttctgtga tagtcgatcg ttaagcgatt cagcacctta cctcaggcac cttcgggtgc
     1621 cttttttatt tccgaaacgc acctcaggag gtgaat
//
LOCUS       DAZFRJ010000411         1655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000411 DAZFRJ010000000
VERSION     DAZFRJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.414"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..249
                     /locus_tag="ACHKUN_005012"
     CDS             <1..249
                     /locus_tag="ACHKUN_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6663092.1"
                     /translation="TRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCR
                     EHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            262..636
                     /locus_tag="ACHKUN_005013"
     CDS             262..636
                     /locus_tag="ACHKUN_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6663093.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            633..1454
                     /locus_tag="ACHKUN_005014"
     CDS             633..1454
                     /locus_tag="ACHKUN_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6663094.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          423 a          338 c          473 g          421 t
ORIGIN      
        1 acacgctggg taaagacaca gccgtgtgcg tgttgtggta agccagcgga cgatcctcat
       61 catctgattg gtcatggtca gggtggaatg ggaacaaaat cccacgatat tttcacgcta
      121 ccgctgtgtc gggagcatca caacgagctt catgcggatc cgctggcgtt cgaagaaaag
      181 catggttccc aggttgattt aatttttcgt tttcttgatc acgcctttgc aaccggcgtg
      241 ctcgggtaaa agaggttact gatgcgtata gagtttgttt tgctttaccc gccgacggtg
      301 aacacctact ggcgacgtcg tggcagcaca tattttgtat caaaagccgg tgagcgttat
      361 cgccgggctg tggcgcttat tgttcgccag cagcggctga aattaagcct gtccggaagg
      421 ctggcgataa agattattgc cgagccaccg gataagcgcc gccgtgacct ggacaatatt
      481 ctgaaagcgc cgctggatgc gctgacgcat gcgggagtgt taatggacga tgagcagttt
      541 gatgaaatca atatcgttcg tggtcagcca gtatctggtg gacgtctggg ggtgaagatt
      601 taccccataa tgcttgaagg gctggtcaaa aaatgaaact ggaagattta ccgaaatact
      661 actccccaaa atcccccggc ctgactgatg catcggcctc aacgtcgaaa gatacgctga
      721 gtatcactga tgtgatggcc gcgcagggca tgacacagaa ttgggctgag atggggtttt
      781 ctgcgttcct tgggaaaatg ggcattagta tgaatgacag agagcgggca acagaattgc
      841 tgacagaata tgcactcagt cggtgtgatc gcgtggcggc gttaagaaaa ctcccggcag
      901 aaataaaacc ggcagtgatg cgtattatgg cttcgtatgc gtttgaagat tatgcccgta
      961 gcgcggcgag caaaaaacag tgcccctgtt gtcacggaaa aaaatttatt gaaagcgagg
     1021 tttttacaaa caagatccag tatccggatg gtaagccgcc agtgtgggca aagtgcacaa
     1081 aaggcgtgta tccgtcttac tgggaggaat ggaaaaaagt cagggaggtg gtaaaagttg
     1141 cctgtccgga gtgtggaggg aagggggagg tttccaccgc ctgtaaagat tgtcgtgggc
     1201 gcggtgttgc cattcatcgt gaagagtcgg taaaacgtgg tatgcctgtt atcagagact
     1261 gccagcgttg tggtggtcgt ggctatgaaa gattaccttc aacggaggca tttaatgcca
     1321 tatgtaatgt aaccgatgcc atatctcttg atacatggaa aaaaacagtt aaacgttttt
     1381 acgatacgct ggtggtgcag tttgatattg aagaagcatg ggcagaacaa caactgaaaa
     1441 aggtgaccag atagctttgt tgatttttcc cgaatctgtg gtaaatttgc cctaacgatg
     1501 ggcgttttat gcctgacgtt agaagatttt ttacacccgt cgccaggcgg gtttttttat
     1561 gactgaaatc acgccagtac agtaaacgcg ctggtggttg tgaataccgg tctttcagct
     1621 tgctggcttt ttcgacaaga gttattggtg tgtca
//
LOCUS       DAZFRJ010000412         1653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000412 DAZFRJ010000000
VERSION     DAZFRJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.415"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..65
                     /locus_tag="ACHKUN_005015"
     CDS             <1..65
                     /locus_tag="ACHKUN_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO6663095.1"
                     /translation="CCAELMSDPNSSMYEEEDDE"
     gene            58..780
                     /locus_tag="ACHKUN_005016"
     CDS             58..780
                     /locus_tag="ACHKUN_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO6663096.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            780..1070
                     /locus_tag="ACHKUN_005017"
     CDS             780..1070
                     /locus_tag="ACHKUN_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6663097.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            1067..1429
                     /locus_tag="ACHKUN_005018"
     CDS             1067..1429
                     /locus_tag="ACHKUN_005018"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6663098.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            1426..1614
                     /locus_tag="ACHKUN_005019"
     CDS             1426..1614
                     /locus_tag="ACHKUN_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO6663099.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
BASE COUNT          500 a          382 c          424 g          347 t
ORIGIN      
        1 actgctgcgc agaactgatg agcgatccga atagctcaat gtacgaggaa gaagacgatg
       61 aatgagttaa taaatggcaa tgccatcaaa atgacaagca ttgaaatagc tgagttggtg
      121 ggtaagcgtc atgacaatgt gaaacgtacc atcgaaacgc tggctaaaaa tggtgttatc
      181 cggcttcctc aaattgaggt ttccgaaaga atcaataact tagggttcaa tgttcagtac
      241 gagcattacg tcttcgaggg cgaacaaggt aagcgagaca gtattgtcgt tgttgcccag
      301 ttgtcgccag agttcaccgc tcgtcttgtt gaccgttggc gagagcttga agaaactgcg
      361 gttaatatcc ccaaaacgtt accggaagcg ttgcgccttg ctgctgatct tgctgagcag
      421 aaaatgcaac tggaaaacca gctcgcaatt gccgcaccta aagttgagtt tgccgatcgc
      481 gttggcgagg ccagcggaat tttgattgga aactttgcaa aggttgttgg tattggtcca
      541 aacaaactgt ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg ttcccggcgc
      601 aatgtgccaa tgcaggaata tatggatcgc ggctatttca cagtgaaaga aacagcggtc
      661 aacacaaatc acggaataca gatatcgttc accacaaaaa tcaccgggcg tggtcaacag
      721 tggctgacca gaaagctgct cgataacgga atgctgaaag taacagggga ggctgcttaa
      781 tggctaacct acgcaaagaa tctcgcggca gagaatgcca ggtacgtatt tacggcatat
      841 gcaatggcaa tcctgaaact acagttctgg cacattaccg gatggctgga atttgcggaa
      901 cgggaatgaa gcctgacgac ctgatcggcg catgggcttg tagcgcgtgt cacgatgaaa
      961 tcgaccgacg cacccataac ctcgacaaca aagacgccag actttaccac ctcgaaggcg
     1021 tgatcaggac gcaggcgata ctgctgaagg aggggaagat taagtcatga acgaatatca
     1081 gtttgtgctt ccatacccgc cgtcggtgaa tacctactgg cgaagacggg gaagccaata
     1141 ctacatcagc gataaaggcc agaaataccg aaaagacgtt cagcaaatca tccaccaact
     1201 caagttagat attttcacca aatcacgact ccgcatcaaa gtaatcgcag acgttccaga
     1261 ctcccgccgc cgcgacctag ataacatcct gaagggttta ctcgactccc ttatccatgc
     1321 cggatttgcg gaagacgacg agcaattcga tgacattcgc gtaattcgtg gtgtgaaagt
     1381 accaggcgga cggcttggaa taaaaatcac cgaactggag aacgcatgaa cgccacaatt
     1441 caaacgatac cagagcttct tatccagaca cgaggcaatc agaccgaagt ggcgaggatg
     1501 ctttcctgcg caagaggaac agtgctcaag tacaaccgag acagcaaagg cgagcgtcat
     1561 gtaatagtta acggcgtcct gatggtcaaa cagggcaaga ggggaagacg atgagactcg
     1621 aaagcgtagc taaatttcat tcgccaaaaa gcc
//
LOCUS       DAZFRJ010000413         1648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000413 DAZFRJ010000000
VERSION     DAZFRJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.416"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1443)
                     /gene="ehxB"
                     /locus_tag="ACHKUN_005020"
                     /gene_synonym="hlyB"
     CDS             complement(<1..1443)
                     /gene="ehxB"
                     /locus_tag="ACHKUN_005020"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO6663100.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDG"
     gene            complement(1493..>1648)
                     /locus_tag="ACHKUN_005021"
     CDS             complement(1493..>1648)
                     /locus_tag="ACHKUN_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663101.1"
                     /translation="NMVNITSVANDINKIISSVSGFDSGDERLASLYNLSLHQNNTHS
                     TTLTTTV"
BASE COUNT          546 a          345 c          275 g          482 t
ORIGIN      
        1 accgtcagaa gaataccgaa atctgacatt tttaaattcg atatccccct gaatttccgg
       61 cagtatattt ctgcctgact ttttctctac cggtgtattt aatacatcac caaggcgctc
      121 aacagaaata ccaacctgct gaaaatcctg ccaaagatga gccagtctga taacaggagc
      181 aatgacctga cctgcaagca tattaaacgc gattaactgc ccgatactta tctctcccga
      241 tatgacaaga tgtgctccca tccatagact aattaccatt acacttttct gtatcagctg
      301 tatcccctgc tgccctgtca ttgcaaccag atttaccctg aaactggaag caacataccc
      361 ggccagttgt ttatcccatg tagcaatcat ctgtggtgat actgccatgg atttaattgt
      421 atttattgct gttaccgttt cgacaagaaa agcttgattt tctgcattcc tgagaaactt
      481 atcatcaaga cgtcgacgta ataagggtga tataaacaat gaccatataa cataacaagg
      541 tagtgacaat aatataacca gtgttaattg agggctgtaa taccacatga cacaaaaaaa
      601 tattacagaa aaaaatagat caagaactga tgtcaacgct tgtccggtta aaaaattacg
      661 aatttgctcc agttccctta ctctggcaac ggtctctccg accctgcgat tttcaaaata
      721 tgaaacaggc aatgcaagca aatgtctgaa taacttagcg ctcagttcga cgtcaatacg
      781 acttgtagta tgagagaaaa tataagttct ggctccggtt aatatcactt caaaaagtat
      841 cactataata aatgctattg tgataatatt taacgttgaa aacccccggt gaactaaaac
      901 cttatccatt acaacctgaa aaaaaagagg tgttattaac gcaagaaact gaataaaagc
      961 agaaacagtt aacacctcaa gtaaaatcct cctgtatttc acaacagagg ggataaacca
     1021 agaaaaatca aatttagcta actctccgac tactgttgct cttgacgtaa ccagaatgac
     1081 ttttccctga tacagttttt caaactcatc ccgactaaaa gttagtgact gatgttgttc
     1141 tggatcataa acaagatagc gtgatgaatc tttagtaaca cgagacaata tataatgctt
     1201 accgtcatcc cgccatatca acgcaggtaa agaaattatt gacaatctgg aaaaatgttt
     1261 ttctacatat tttgctttta agccaatgga tttcgctgcc agtaaccatt cagtcacccc
     1321 aaaatcttgt gtgtgggtat tatactgatg ccttatagtt tcagcattga cagttatatt
     1381 atgatattgt gcaagcaata tcagtgcata cagactatta tgagaactac atttactcat
     1441 catataacga tgaccattcc tcctggaatg gccatcacct cctcttttag tctcagacag
     1501 ttgtcgttaa agttgttgag tgtgtgttgt tttgatgtaa ggataaatta tataaagatg
     1561 ctaatcgttc atcacctgaa tcgaaccctg aaactgaaga aataatctta ttaatatcat
     1621 tggcaacact ggtgatatta accatgtt
//
LOCUS       DAZFRJ010000414         1644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000414 DAZFRJ010000000
VERSION     DAZFRJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.417"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..393
                     /gene="nleG7"
                     /locus_tag="ACHKUN_005022"
     CDS             <1..393
                     /gene="nleG7"
                     /locus_tag="ACHKUN_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO6663102.1"
                     /translation="AQISQDFMLQLSSNIGWKKGAENALKNKIHSHSFVVNPDEFSCD
                     TQFLKCPITLCVPEKGVFVKNALNSNICTLYDKSAFMNLTREHLPHPLSREKIVKEMI
                     IERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            complement(618..1493)
                     /locus_tag="ACHKUN_005023"
     CDS             complement(618..1493)
                     /locus_tag="ACHKUN_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO6663103.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT          483 a          330 c          308 g          523 t
ORIGIN      
        1 gcacaaatat ctcaggattt tatgcttcaa ttatcttcaa atataggatg gaaaaaaggg
       61 gccgaaaacg cccttaaaaa taaaatccat tctcattcat ttgttgtaaa tcctgatgaa
      121 ttctcttgcg acacacaatt tcttaagtgc ccaataacat tatgcgttcc agaaaaaggg
      181 gtttttgtca agaacgcact gaactccaac atatgcactc tttatgataa gtctgcgttc
      241 atgaatctca caagagaaca tctaccccac cctctcagca gggaaaagat agtaaaagaa
      301 atgattattg aaaggaatat gtgttatttt gacaccataa gtcagcattt cataattatg
      361 gatgcagacc aacagaaaca gcattgtaaa taaaatgtaa taattacata ctattagtga
      421 ttctcatgca tcgtaagcgg ctcgccagaa ccgtattgat atttactgag cgctcagatc
      481 aactttccat ggcaacagat cgcgtacccg gtttgccggc cagtcctgga tatgctcccg
      541 ccgacaatat taatggccag gaaatgttta aaacaatgat tctgtttcct aaccatcaag
      601 tgtatatgct ggctcaatta ctcaatatct atgggttgag ttctacccaa agtggctctg
      661 tagtcgcttc ttcttacagt atgttcaggt gttgcaggca cagttgtgga gcgtagatgc
      721 gttgttggtt taccatgctc tggtacataa aaaacgccag tgccatggta acctgatata
      781 gaaacgttaa ttccgcgacg ttcaaactcg gcatagacgt gttctgctaa agacctcttt
      841 tgaccaaata atgccctggc caaccagccg ttattcatat ttgcggattt ttctatttca
      901 tcaggagaga agtctttgtt ttttattatg ttggctgagt tacaggatgt taatctgata
      961 tcatctattt catgaagatt atgttgaaca atgcggtcga caatatctga cggtgataaa
     1021 aattcatctc cgcacttaag aagaggtaga ccagcagaac catgcccaga cagataaatt
     1081 ttgttgtatt ttcctggtgt taaatcatct ggcaggatgg ttttcatctc tgtcgctgta
     1141 atacttatag ctgcagcagc aacaacatcg ctattacttg attgtagatg gcttttattt
     1201 gctccaggat atgtaaactc cattttcctt ttatcaaaat cttgttttgt agcatcattc
     1261 aggaataata aatcatacgg ttttttcccc gttgtgactc ttgaaacatt ttggccaaga
     1321 atattaagag tatagtcatt aacggatttc attcctaaaa aaacaagtaa tccttctttc
     1381 tcttgcgctt tttcgattgt tctccctgct aaagttattg ggatggattg agattgtttt
     1441 gtcggaactg atacgctggc gctaataggt agtagggttc ttatgctaaa catacaacct
     1501 ctttcttaat tttcgaacct gtttaggatt ctgtgtaaat tcaaaataaa cctgtctgaa
     1561 cattcctaac aaacatccac cggacatgac aacaaaaacc ggagccggac tccggttttt
     1621 gtgaagctgt cggctatttc attc
//
LOCUS       DAZFRJ010000415         1643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000415 DAZFRJ010000000
VERSION     DAZFRJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.418"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..1637
                     /locus_tag="ACHKUN_005024"
     CDS             99..1637
                     /locus_tag="ACHKUN_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO6663104.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT          371 a          472 c          497 g          303 t
ORIGIN      
        1 tcgactggcg gcagcctaaa agactgctta cgtccctgac tatgttgtaa gcctctttat
       61 cctggtcgac gctgaatgag cctggtaata tacccggtat gagcagctca cttcctgacg
      121 atatcaatgc actgaaacgt ctccttgccg aacaggaggc gctgaaccgt gccctgctgg
      181 aaaagctgaa cgagcgtgaa cgcgaaatag accatctgca ggcacagctg gataagctgc
      241 gccggatgaa cttcggcagc cgctccgaaa aagtctcccg tcgtatcgca cagatggaag
      301 ctgacctgaa ggcacttcag aaagaaagtg atacccttac cggtcgggtt gacgacccgg
      361 ccgtgcagcg cccgctgcgt caaacccgca cccgcaaacc gttccccgaa tcactccccc
      421 gcgatgaaaa acggctgctg ccggcagcat catgctgccc ggaatgtgga ggctcactga
      481 gctatctggg tgaggatgcc gccgaacagc tggagttgat gcgcagcgcc ttccgggtta
      541 tccggactgt acgtgaaaag catgcctgta ctcagtgcga tgccatcgtg caggcccccg
      601 cgccttcacg gcccatcgag cggggtatcg caggaccggg gctgctggcc cgcgtgctga
      661 tctcaaagta tgcagagcac accccgctgt accgccagtc tgaaatgtac ggccgccagg
      721 gcgtggagct gagtcgttca ctgctgtcgg gctgggtgga tgcatgctgc cggctactgt
      781 caccgctgga agaagcgctt caggactatg tgctgactga cggtaagctc catgctgatg
      841 acacgcctgt cccggtgctg ttgccaggca ataagaaaac gaagaccggg cggttatgga
      901 cctacgttcg tgacgaccgt aacgccgggt caacgctggc gccggcggtg ttgttcgctt
      961 acagcccgga cagaaaaggc atccatccgc agacccatct tgcggggttc agtggtgtac
     1021 tgcaggcgga tgcatacgcc gggttcaacg agctgtaccg ggatggccgg ataacggaag
     1081 ccgcctgttg ggctcacgcc cgccgtaaaa tccacgatgt gcacgttcgc accccgtcag
     1141 ccctgacgga ggaagcgctg aaacggatcg gcgaactgta cgccatcgag gcagagataa
     1201 ggggaatgac ggcggagcag cgccttgccg aacgtcagtt gaaaacgaaa ccgctgctga
     1261 aatccctgga aagctggctg cgtgaaaaga tgaaaaccct gtcgcgacac tcagaactgg
     1321 cgaaagcgtt cgcatacgcc ctgaaccagt ggccggcgct gacgtactat gcagatgatg
     1381 gctgggctga ggcggacaat aacatcgctg aaaatgcgtt gcggatggtc agtctgggcc
     1441 gcaaaaacta cctgttcttc ggttcggatc atggaggaga gcggggagcg ctgctgtaca
     1501 gcctgatcgg gacgtgcaaa ctgaacggag tggagccaga aagctacctc cgctatgtcc
     1561 ttgacgtcat agccgactgg ccgataaacc gggtcggcga actgctcccc tggcgcgtag
     1621 cactgccgac tgaataacac atc
//
LOCUS       DAZFRJ010000416         1642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000416 DAZFRJ010000000
VERSION     DAZFRJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.419"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            315..1292
                     /locus_tag="ACHKUN_005025"
                     /pseudo
     CDS             315..1292
                     /locus_tag="ACHKUN_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          475 a          379 c          384 g          404 t
ORIGIN      
        1 ataagaataa taatatttga tcacaactga agatgctttt tacaatgaag aattattgtt
       61 aaaaaacgct ttgttgaatc aaccgaactg ttgctgagtt gcaggatcac atcacgtatt
      121 ttcctgaaaa cgcaggctgc ttagtagcaa agcagaagtt caaaatcacc aactaaatag
      181 ctgcgccgaa tagtagatca atgaaaggga actcagcccg gattgtgcga tctgatcaat
      241 cgccaaaaat ccccccctaa ccggactgag cgatgccgat catagcacca attccccgcg
      301 acgaatgacg cctgatgcaa aaagcaatct ataaaactca cgataaaaat tatgcccgca
      361 ggctgaccgc catgctgatg ctgcaccgga gcgctcgcgt cagtgacgtt gccagaacgc
      421 tctgctgcgc ccgttcctcc gtcaggcgct ggattaactg gttcacgctg tcgagtgttg
      481 aaggtctgaa atcattaccc gcaggacgtt cccgccgctg gccgtttgag catatctgca
      541 cattgtttcg tgaactgata aaacactctc ccggatattt tggctatcag cgttcacgct
      601 ggagtacaga actgctggca ataaaaatca acgagataac cgagtgccag ttacatgccg
      661 gaaccgttcg ccgctggttg ccattagtcg ggcttgtgtg gcgcagggct gcgccaaccc
      721 tgcgtacccg taacccgcat aaagcagcaa ttcacaaagc actggacgaa tgtagtgcgg
      781 agcatccggt cttttatgaa gatgaagtgt atatctatct taatcccaaa accggtgcgg
      841 actggcaact tcgcagacag caaaaacatg tggtaacacc ggggcagaat gaaaaatact
      901 atctggctgg tgctttacat agtggaacgg gcaaagtcag ctatgtgggt ggcaacagca
      961 aaagctcagc cttatttatc agtctgttga aacatctgaa attgacatac cgacgggata
     1021 aacctatcac gctcatagtg gacaactaca tcatccataa aagtcgggaa acacagcgct
     1081 ggctgaaaga aaatccgaag ttcagagtga tttatcagcc agtttactcg ccgtgggtga
     1141 atcatgttga gcggttatag caggcgcttc acgatacgat aacgcgcaat catcagtgcc
     1201 gttcaatgtg gtaactgctt aaaaaggttc attattttat ggaaaccgtg agcccattcc
     1261 ccggtggtaa atatgggctg ttaaaagtgt agcggtattc ggcgcagcta tttaggatgg
     1321 tcaggtcgca gaagttctgt gccactacag tgcgtgtact aaacaaaatg acgagggcac
     1381 gaatgtcata atacgtgcgt attacctgca gtagctacca actgaggggg cttatcccaa
     1441 acctgattta ttcaacaaag ctggactgga cttatcctta ctctgaatta ttttccagac
     1501 atatgcatca tgtactggct cgcttggttc aggaaataca ggagcaaatc tacagctatt
     1561 agatttattc ctatttgtat gcaaagtcgc attgcataat gcggagctat gttacagata
     1621 cgcctgacgt gcgtagtaaa at
//
LOCUS       DAZFRJ010000417         1636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000417 DAZFRJ010000000
VERSION     DAZFRJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.420"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..202
                     /locus_tag="ACHKUN_005026"
     CDS             <1..202
                     /locus_tag="ACHKUN_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663105.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
     gene            210..656
                     /locus_tag="ACHKUN_005027"
     CDS             210..656
                     /locus_tag="ACHKUN_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO6663106.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            653..1180
                     /locus_tag="ACHKUN_005028"
     CDS             653..1180
                     /locus_tag="ACHKUN_005028"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO6663107.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1177..1359
                     /locus_tag="ACHKUN_005029"
     CDS             1177..1359
                     /locus_tag="ACHKUN_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6663108.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
BASE COUNT          440 a          344 c          451 g          401 t
ORIGIN      
        1 tgagttgatt ttttctgcat tgaggattct cggtgctatg tggatggtgg cgacgttcat
       61 tgtggttgcc agcagttttg tccggctggt aggcgaaggt aaagacctgg ttggtgtgct
      121 tttcggtagc attttcctgt gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaggtgacaa tgaagcaaat atacatgctt cgcaacgaag
      241 caatcagaaa caacgccata gacgcaatac tctcacttcc gatcgacgac aagtcacctc
      301 acgaagtcca cattaaagaa ccaaagcgga gcaatcctca aaaccgcctt atgtgggcgt
      361 tattgcagga cgtatcacgt caggtgcttt ggcatggaca gagacttgcg ccggaggact
      421 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa cgaagtgtgc
      481 ctggtatcga tggtggcgtt gtcatgcttg gcgtgcgtac cagcaaaatg cgaaaggcca
      541 gcatgactga gcttatcgaa atcatgtttt ggttcggctc agagcgcaac gtgcggtgga
      601 gtgatgactc ccggcgagag tatgaatggt cacaacgaaa aggtagggct gcatgactat
      661 caaatcaaat acaccagcac acgacaagga ctgctggcaa acgccgcttt ggctttttga
      721 tgcgctggat attgagtttg gattctggct ggattcagct gcgagcgaca aaaatgctct
      781 gtgcgctcac tggctaactg aggccgacga cgcactcaat tctgagtgga taagccacgg
      841 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
      901 gcagtgcata caacagcgac agacggtagt gatgcttgtg ccagaggata tgtctgtcgg
      961 atggttcagc aaggctctgg agagtgttga cgaagttcgc attatcactg atggacggat
     1021 taattttatc gaaccatcga cagggctgga gaagaaggga aacagtaaag gctccatgct
     1081 gctgatttgg cgaccgttca tcagtcctcg acggatgttt actaccgtat ccaaagcggc
     1141 attgatggcg atcgggcagg gcgtcaggag ggcggcatga ggcgacagcg acgaagtttc
     1201 accgacatca tctgcgaaaa ctgcaaatac cttccaacga aacgctacag aaataaacgc
     1261 aagccaatcc caaaagaatc tgacgtaaaa accttcaatt acacggctca cctgtgggat
     1321 atccggtggc taagacatcg tgcgaggaaa acaaggtaat tgactaaaat cgaagttacg
     1381 aacaagaaag cgtcgagcgg gcttcagtgt acactgagtg gattctatct aggcttagtg
     1441 catacagaag attgctggta aaggacatgc caggcaaaac gatgaggact gatatttatg
     1501 aaaacatctg attttttact gttcttgcat gcggtacagg aggggctttg accgggcatt
     1561 ttatcgtgaa tattttcact tggtatttct ttggttttag agattacttc actcgatggg
     1621 ttttaaatag ttttcg
//
LOCUS       DAZFRJ010000418         1623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000418 DAZFRJ010000000
VERSION     DAZFRJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.421"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            148..1377
                     /locus_tag="ACHKUN_005030"
     CDS             148..1377
                     /locus_tag="ACHKUN_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6663109.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          413 a          357 c          334 g          519 t
ORIGIN      
        1 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagaggggt
       61 agatagggcg ttaatctccc atacttaacc tggtttatgg taaattgccc tccattttgt
      121 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
      181 tggagtttcg cgctctacgg cacagccgtt ggtgcaggca cgctcttcct tcctattcag
      241 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
      301 tattggccac ataaagcctt atgccagttc atcctctcat cgaaaacatc agcaggtgaa
      361 gggataacgg gcgcagtaac acactactat ggcaagaaga ttggtaatct gattaccacg
      421 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
      481 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
      541 ctgggtgttg tattaatcct gaatctcatt tttctgatgg gacgccatgc aaccattcgg
      601 gtaatgggtt ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
      661 gtcggtagtt ggcaacctga tctattaacg acccaggtag agttcaatca gaataccctt
      721 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
      781 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
      841 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
      901 tgcctgcttt ctattccacc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
      961 ttatcggcgc tttctatgct gccgaacgcc ccagcatggc tgtcaatttc cgggattatt
     1021 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
     1081 gccacggagg tcgtcaaaac aacactacag caggttggtg taaagaaaag tcgtgcattt
     1141 aaccgcgcac tatcaattat gttggtatcg ctgatcacct tcattgtttg ttgcattaac
     1201 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
     1261 atcatgccta cgttgtcaac gtatctgatc ccggcgctta aaccctggcg ttccatcgga
     1321 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctgatat
     1381 ccattgtgaa tggttcaaaa ttgctacaat tttgaaccat tccagttttc ataatgttca
     1441 ttcgttatta ttccgcacta tcacttccct catcatatcc tagtattatc agttagttaa
     1501 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
     1561 caagatatct tccggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
     1621 tag
//
LOCUS       DAZFRJ010000419         1617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000419 DAZFRJ010000000
VERSION     DAZFRJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.422"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1607
                     /gene="tir"
                     /locus_tag="ACHKUN_005031"
     CDS             <1..1607
                     /gene="tir"
                     /locus_tag="ACHKUN_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO6663110.1"
                     /translation="NLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMGSRLL
                     FTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNSAIGS
                     SLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRGGAGH
                     AMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLRADPK
                     LWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLTKEAF
                     QNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQKYDE
                     QHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVVDNQP
                     TNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRNDSLA
                     RISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGLRLGM
                     GGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          443 a          371 c          428 g          375 t
ORIGIN      
        1 gtaatcttgg ccacaatccc aatgtgagag ctttaattcc acctgcaccg ccattacctt
       61 cacaaaccga cggtgcagga ggtgcccgta atcagctcat taactcaaat ggcccgatgg
      121 ggtctcgttt gctatttacg cctataagga attctgttgc tgatgctgct gattctcgtg
      181 ccagtgatat tcccggactt cctacaaatc cactgcgctt tgctgcgtcc gaggtatctt
      241 tgcatggtgc gcttgaagtt cttcatgata aaggggggct tgatactctt aactctgcta
      301 ttggatcttc gttattccgt gttgaaactc gggatgatgg cagccatgtt gctatcgggc
      361 aaaaaaatgg cctcgagacc actgttgttt taagtgagca agagttttct agcttacagt
      421 cccttgatcc tgaaggtaaa aacaaatttg tatttactgg aggccgcggt ggcgcagggc
      481 atgctatggt cacggttgct tcagatatcg ccgaagcccg tcagaggata atagataaat
      541 tagaaccaaa ggatacaaag gagacgaagg agccagggga tccaaatagt ggcgagggaa
      601 aaatcattga aattcatacc tcaacctcaa cttctagcct ccgtgcagat cctaaacttt
      661 ggttgtcatt ggggactatt gctgcaggtc tgatagggat ggctgcgacg gggattgcac
      721 aggctgttgc gttgactcca gagccggatg acccaatcac taccgaccct gatgctgcag
      781 caaacacagc tgaagcagcg gcaaaagatc agttaacgaa agaagcattc cagaacccag
      841 ataaccagaa agttaatatc gatgagaacg gaaatgcaat tccgtccggg gaactaaaag
      901 atgatgttgt tgcgcaaata gcagaacaag ctaaagcggc gggtgaacag gccagacagg
      961 aagctattga aagtaattct caggcgcagc aaaaatatga tgaacagcat gctaaacgcg
     1021 aacaggaaat gtctctttca tcgggggttg gctacggtat tagtggtgcg ctgattcttg
     1081 gcgggggaat tggtgccggt gttactgctg ctcttcatcg gaaaaaccaa ccggcagaac
     1141 aaacaatcac tacacgtacg gtagtcgata atcagcctac gaataacgca tctgcgcagg
     1201 gcaatactga cacaagtggg ccagaagagt ccccggcgag cagacgtaat tcgaatgcca
     1261 gcctcgcatc gaacgggtct gacacctcca gcacgggcac ggtagagaat ccgtatgctg
     1321 acgttggaat gcccagaaat gattcactgg ctcgcatttc agaggaacct atttatgatg
     1381 aggtcgctgc agatcctaat tatagcgtca ttcaacattt ttcagggaac agcccagtta
     1441 ccggaaggtt agtgggaacc ccagggcaag gtatccaaag tacttatgcg cttctggcaa
     1501 gcagcggcgg attgcgttta ggtatgggag gattaacggg ggggggcgag agcgcagtaa
     1561 gtactgccaa tgccgcacca acgccgggac ccgcacgttt cgtttaaata tatccgt
//
LOCUS       DAZFRJ010000420         1604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000420 DAZFRJ010000000
VERSION     DAZFRJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.423"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1604)
                     /locus_tag="ACHKUN_005032"
     CDS             complement(<1..>1604)
                     /locus_tag="ACHKUN_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6663111.1"
                     /translation="PGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPETGT
                     STVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVATYGVWGLSLPSLRRRLFRCVSI
                     RENTDGTFAITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTAEVTADSGEY
                     QVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQLAPGNYRLTVR
                     AVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEK
                     RITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASD
                     DAEGYLDFFKGKITESHLGKELLEKVDLTEDNTSRLDEFSKEWKDANDKWNAMWGVKI
                     EQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFM
                     NDVFLKRLTAPTITSGGSPPAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCR
                     VLGKLSANQIEGDLVKTVGKAFPRDSR"
BASE COUNT          314 a          490 c          427 g          373 t
ORIGIN      
        1 cgggaatccc gggggaaagc tttgcccact gttttaacga gatcgccttc aatctggttc
       61 gcggacagtt ttcccagaac ccgacagttc tcattaatcg tgacgttgtt gagcgtcccg
      121 gagttcgcat tcacactacc actgatatcc gcatttttag cagtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg actgccacca ctggtaatgg tgggggccgt caggcgcttc
      241 aggaacacgt cgttcatgaa tatctggttg ccctgcgcca caaacatcgg agtttcattc
      301 ccgtttgccg ggtcaataaa cgcgatacgg ttagcggcaa ccagaaactg gctcagcttg
      361 ccttcctccg tgtcctccat gctgaggcca atacccgcga cataatgttt gccgtctttg
      421 gtctgctcaa ttttgacgcc ccacatggca ttccatttat cgttagcgtc cttccactct
      481 ttcgaaaact catccagtct gctggtgtta tcctccgtca gatcgacttt ttccagcagc
      541 tccttgccga gatgagattc ggttatcttg cctttgaaaa aatccagata accttccgca
      601 tcatcgctcg cccgaccgac ggcctccacg aatgccgatt tgccaacagt gttcacactg
      661 cggatataaa agtaataatc atggcccggt ttgatattga tactggcggc tatccagtac
      721 agccccgtgc caaggtagcg ggctgtggtt tcaacctgcc tgatatcggt aatccgcttt
      781 tccgagaacc agaactcaaa ctgtaccgtc gggtcataaa cggcaagatg cggcgtggcg
      841 gttatctgaa aatagcccgg cgtcagctca atcctcgacg gtgctgccgg tgcggcaatc
      901 cggaacgata ccgacgccgg atcaccctgc tgcccccacg catttaccgc ccggactgtc
      961 agcctgtagt tccccggcgc cagttgcctg aagcggtatg tggtttccgt cgtccgggcc
     1021 gtgctgacca gccgctcact gccgtcatcc gctgttacgg tcagacggag caggaagctc
     1081 acgcccttca ccaccttcgg cgtgtcccag cgcgccagca cctgatattc cccgctgtct
     1141 gcggtgactt cggcagtcag gtgctgcact gccggcggga tgacgctgtt cagtgagccg
     1201 gacatcggct caaagcgggc cccgttatcc acaatggctt ctttttccgg cacatgctgc
     1261 accgccgtga tggcaaaggt gccgtccgtg ttttcccgga tggaaacaca gcggaacagg
     1321 cgacgacgca gtgacggcag ggagagtccc cacacaccgt atgtcgccac gccatccggc
     1381 agggtactga cctgtatccg gtccggcgcg gggtgtgcgg tgatgtccac actcaccggc
     1441 ttaccgctgc cgttaatcag gttcaccgtc gatgtacctg tctccggcag tgtcacctca
     1501 cggtccagcg tcagggtgcg gctggcggca tcgatggaca ggatacgtcc gccggtcagg
     1561 gtcccggcat agtcgttatc acagatttca ataatgtcac cggg
//
LOCUS       DAZFRJ010000421         1585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000421 DAZFRJ010000000
VERSION     DAZFRJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.424"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..848)
                     /locus_tag="ACHKUN_005033"
                     /pseudo
     CDS             complement(1..848)
                     /locus_tag="ACHKUN_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            825..1066
                     /locus_tag="ACHKUN_005034"
                     /pseudo
     CDS             825..1066
                     /locus_tag="ACHKUN_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1147..1305)
                     /locus_tag="ACHKUN_005035"
     CDS             complement(1147..1305)
                     /locus_tag="ACHKUN_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6663112.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1391..>1585)
                     /locus_tag="ACHKUN_005036"
     CDS             complement(1391..>1585)
                     /locus_tag="ACHKUN_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663113.1"
                     /translation="GYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLSTSFNAGSKRF
                     VIYQSAQDKNLQVDIVRTRI"
BASE COUNT          442 a          430 c          344 g          369 t
ORIGIN      
        1 ccacggcaca tctgcagcac tgccaccatg gcactgcgcg ttaaacacgg agaggtcagc
       61 acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc tgtacgtggg tggcggcgct
      121 catgtcaaac tcaccctgca tccagcagac ggccagcaga acgtttttgg gatttttctg
      181 caatgccgct tttgtgcggg aaatcagatc ctgatataac ggcttgccca ccccccagcg
      241 tgccgaatcc tgactggccc ccgtggactc gctgaatgtc ccctccgcgc cctgggtaaa
      301 tgccgaacca ccacgacagc atggtaccag caggatcccc gcgttattcg ggatatacgg
      361 gagcagtttt ttggcaatat gtaaaccctg gccgacacag ccgtactgcc ctttgctcag
      421 gtcagccctc ggatgattca gcgtactcat atcctgcaca tcatgcagac agtggtcagc
      481 cggaataata tcgttatatc tgcaggcagc cccgcccggc gtcactgtac tgcggcgcgc
      541 cagctgttta atgcgcggat ccggagcatc gtatgaatcc ggcagcggaa gcccttcacc
      601 gtaagccctt caccgtaagc catggcattg gattgcccgg ccagtacgat gacgtagtac
      661 caatccggct cagatgaagg gccgacctgt ggctctcctt caatagccac cgcctgcatc
      721 agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg
      781 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc gtaatgttta
      841 atcgacatcg aatttctccc gtgtagagga acagagttaa aaagccggaa gcggaatcaa
      901 atcacaggat gaccatctgc cagtggcagg tcataaaaaa aaggctgcgc aatgcgcagc
      961 cagaactcac aaggaaaatg ataaaaggaa taacactagt gatgtacgca tggcgcctcc
     1021 cgctaagttc tgcaatgatc aaacagaact cgctacgtgc ccttaaaact cgatcattta
     1081 gcccctccaa ggaggattca ccatgcggtt gattttttaa taaacagtaa acaaaaaagt
     1141 caagaattat tcattctgtt ctttcatcat cggccacagc aataccacaa tgccgcagac
     1201 cagagcgcca tcagtcagta ccaacattat cctgctggtg aaatccatca tcaccatcac
     1261 taaaagcagg atcacaacag caagcagaca cagtttataa aacaatgttc agaaaacgca
     1321 ttcagcatgc ctaaggttct attcctacga atagccaact tgcaacttaa aatattattt
     1381 atgcagccaa ttaaattctg gtccttacaa tatcaacctg aagattctta tcttgtgctg
     1441 attgataaat gacaaacctt ttactacctg cattgaaaga agtagacaaa accagacaat
     1501 tatcataacg agcaagaaca taataccaac catcattata attaatcatt tcatattctt
     1561 tcttaaactg tggtttgtaa tatcc
//
LOCUS       DAZFRJ010000422         1584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000422 DAZFRJ010000000
VERSION     DAZFRJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.425"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..641
                     /locus_tag="ACHKUN_005037"
                     /pseudo
     CDS             1..641
                     /locus_tag="ACHKUN_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    270..386
                     /locus_tag="ACHKUN_005037"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1164..>1584
                     /gene="ehxC"
                     /locus_tag="ACHKUN_005038"
                     /gene_synonym="hlyC"
     CDS             1164..>1584
                     /gene="ehxC"
                     /locus_tag="ACHKUN_005038"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO6663114.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKK"
BASE COUNT          431 a          285 c          396 g          472 t
ORIGIN      
        1 acacgttttt cccccgaggt ccgtcaacgg gcagttcgta tggttctgga aagtcagggc
       61 gaatatgact cacaatgggc ggcaatttgt tccattgccc caaagattgg ctgtacacca
      121 gagactctgc gtgtgtgggt tcgtcagcat gagcgggata ccgggagtgg tgatggtgga
      181 ctcaccaccg ctgaacgtca gcgtctgaaa gagctggaac gtgaaaatcg tgaactgcgc
      241 cgcagtaacg atatccttcg ccaggcttcc gcttattttg cgaaggcgga gttcgaccgc
      301 ctctggaaaa aataatgcct ctgctggata agctgcgtga gcagtacggg gtcggaccgg
      361 tatgcagtga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc
      421 atcctgataa acgcagtgcc cgtgcgcagc gcgatgactg gctgaagaga gagatacagc
      481 gcgtatacga tgaaaatcat caggtatacg gtgtgcgtaa agtctggcgc cagttgttac
      541 gcgaaggtat cagggtggcc agatgtacag tggcgcgcct catggcggtt atgggacttg
      601 ccggtgttct ccggggtaaa aaggtccgta ctaccgtcag ccgatttacg ggccgtaagt
      661 aacgaagttg gatgcaaatg tcagatcgtg tgcgcctgtt atggctcggc tggtaagaga
      721 gccttatagg cgcatttgaa aaaactccag ctataccgga agatatttat tattaacagg
      781 agaggtttgc ttttctgtgt aggtttagat tttgtgtttg cagggtaaac aaaactgatt
      841 aactcgtcat ttcggtaact gaaatagttt gctgtattct gtttacaatc tctttaataa
      901 ggcatttgtc acgtggctat tcatatgaaa atcatacgtt atcttacaaa tcaatcatct
      961 gagtgttata atataactta gctgtgatat gtgtaagaat gtttaggcaa tatattatta
     1021 tcgctaatgt tgttttggtt gggagagatt ctccttgcgg tagctttttg tttttagatg
     1081 cttcttgctt aaaaagatat aatttctgtt cttttatata ggattcttta catttactga
     1141 gttttcttat ttatttaaac attatgaaat ctaatgcttt tgacgtcatg gggaaggttg
     1201 catggctatg ggcctgttct cctctgcata aaaaatggcc attatctgtt tttgctatta
     1261 atgtcatacc tgctattcag acaaatcaat ttgctctctt aataaaggat gagcttcctg
     1321 tagctttttg tagctgggcc agtttagatc tggaatgtga ggtaaaatat ataaatgatg
     1381 taacatcttt gtatgcaaaa gactggatgt cgggagaaag aaagtggttt attgactgga
     1441 tagctccatt tggacacaac atggagttat ataaatacat gaggaaaaag tatccttatg
     1501 agttattcag agcaataagg ttagatgaga gcagtaaaac aggaaaaata gctgaatttc
     1561 atggaggggg gatagataaa aaac
//
LOCUS       DAZFRJ010000423         1574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000423 DAZFRJ010000000
VERSION     DAZFRJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.426"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..354)
                     /locus_tag="ACHKUN_005039"
     CDS             complement(<1..354)
                     /locus_tag="ACHKUN_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663115.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEV"
     gene            complement(645..1220)
                     /locus_tag="ACHKUN_005040"
                     /pseudo
     CDS             complement(645..1220)
                     /locus_tag="ACHKUN_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(1333..1572)
                     /locus_tag="ACHKUN_005041"
     CDS             complement(1333..1572)
                     /locus_tag="ACHKUN_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6663116.1"
                     /translation="MCGCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVIT
                     QVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          436 a          440 c          267 g          431 t
ORIGIN      
        1 cacctcacca ggacagtgaa gtttttcaat atcaacagta aaggtacatt ccgatatttt
       61 ggcctgcaaa gagttctctt gtcgctgcgc caccattcca ggaagcacgc ttaacaccct
      121 gtttctgagc gcatcaactc catcattaaa accaatattc aataattctg cataaaccag
      181 tcccagccct tcactttctg ctaaaaaacg gcctgtatct ggcatataag aaactctaac
      241 tctctcccct cccaggagaa atcctccaat ctgcccttcc attacctgct gacgcatttc
      301 cgccatctca ttcgcgaatg aagtcgatga tatatatgat actaatccag gcattaccat
      361 attccttcat acagataaat atttcctctg ggcttcataa caaacatctc cctgacatga
      421 caacaaaaac cggagccgga ctccggtttt tgtgaatccg tcggctattt catcccgcca
      481 atatttcccc gtcagcacgc cagatttgca gcggcctcac cactcactgt atatgctttt
      541 tagctgcatc cagtacacca attaccacat ccttataccc tgaacatctg tagcatttca
      601 tataagcaac tggcagttaa catatagctt gggtaatatt tatttcactt atcctttatg
      661 acaaagtttc ctttggtttg gtcataaata cattgttctt gacttacaat cattgatgac
      721 gttattggtt ctcgtgttag cgggtgcttc ccaccgtcgc gaaccaaatg agaaaaagaa
      781 accgaatcaa ataaggtaca aaccaaagaa ccttctgaat ttttaacaaa cacaccttct
      841 tctggtttat ccagtataat tgggcatcga accgcctcgg atggacactg aaggctctcc
      901 gtattaacag aaaacttaca ttgtgatatt ttcccctcaa tatcaccatg tgattgtgtg
      961 ttacctgaac ctgaaaccat tcttaacatt atatccctga gcgcttcagg accaccatta
     1021 agccctgtaa gtaataatcc agaaagcaaa ctgttattgc ccccactcgc cagaaagcga
     1081 ccatccgttt cactatatgt gatacggacc gtctacccgc ctactgtaat ttctccaccg
     1141 ccactggcgg ccatttgagc acgaagaacc tgcattccgg tggaaaatga attggatgca
     1201 atagctgatg ttaacggcat gacaaaactc cctatttaaa tattcattcc aaacacaggg
     1261 agtcataaca aacagccccc atacatgaca acaaaaaccg gagccggact ccggttttgt
     1321 gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag cacgcagaat
     1381 ttgcagcggt cttaccacac actgtatctg ctttttatcc gcatccagta tcaccacctg
     1441 cgtgattacc ctgtcctgct ccgggataat accattctca tcggactcca ggatgtctgc
     1501 cggccccagt cgcagttgtg ctgtaagcga ctgcacgtgt tcacggccat catgctttcc
     1561 gcaaccacac agac
//
LOCUS       DAZFRJ010000424         1571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000424 DAZFRJ010000000
VERSION     DAZFRJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.427"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..315
                     /locus_tag="ACHKUN_005042"
     CDS             64..315
                     /locus_tag="ACHKUN_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6663117.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            335..>1571
                     /locus_tag="ACHKUN_005043"
     CDS             335..>1571
                     /locus_tag="ACHKUN_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO6663118.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLIKPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVTLL"
BASE COUNT          509 a          320 c          378 g          364 t
ORIGIN      
        1 gtcgccggtt gtgactggta ttctgcaacc ggcgctcgtc tgatgtaaga gataaagaaa
       61 gcgatgagcg aagtaatcat gatcgtctct cccggcaaat gggtatccga agagcagtta
      121 attgcgctga aaggaataaa aaaaggtacg ttaaaaaagg cccgggaaaa atcgtttatg
      181 gaaggaaggg aatataagca tgtcgctcat gacggtatgc catgggataa cagtccatgc
      241 ttttacaacc tggaagaaat tgatcgctgg attgagcgcc aggcatctgc aagaccaaga
      301 cgtcatcttg cttgactaaa agcaacacga accaatgaga gaagctgaaa tgaaatatcc
      361 gacaggcgtg gaaaaccatg gagggaaatt acgtatctgg tttgtttata aaggtgtaag
      421 agtccgggaa aatcttgggg ttcctgacac agcaaaaaac aggcgcattg caggtgagct
      481 acgcgcctct gtttgttacg caataaaaac tggcgctttc gactatgcaa aacagtttcc
      541 cgcctcacac aatctggaaa aatttggtga ggcccgacaa gatttaacca taaaagaact
      601 ggctgaaaaa tttctggcac tgaaagaaac tgaagtcgca aaaacgtcac tcaacacgta
      661 ccgtgccgtc atcaaaaata ttctgagcat aatcggtgaa aaaaatcttg catcatcgat
      721 taataaagaa aaattgctgg cggtacgtaa agagttactg actggatacc agatccccaa
      781 aagtaactat attgttacac aaccagggag atcggctgtt accgtaaata attacatgac
      841 aaatctttac gccgtgttcc agtttggtgt tgataacggt tatctggcag acaatccatt
      901 taaggggatc tcaccattaa aggagtcgag aaccatcccg gatccacttt cgcgggagga
      961 gtttatccgc cttattgacg cgtgcagaaa tcagcaagcc aaaaatttat ggtgtgtttc
     1021 cgtttatact gggattcgcc ctggtgaact ctgtgcgctt ggatgggaag atatagatct
     1081 gaaaaatgga acaatgataa tcagaagaaa tctggcaaaa gaccgtttta cagtaccaaa
     1141 aacacaggcg ggaaccaatc gtgtgattca ccttattaag cccgcaatcg acgctctccg
     1201 gagtcagatg gcactaacga gactgagcaa agagcatatc atcgatgttc acctcagaga
     1261 attcggcaga acagagaaac aaaaatgtac ctttgttttt caacctgaag tgtcagcgaa
     1321 agtaaaaaat tatggtgacc attttaccgt tgactcaata aggcagatgt gggacgcagc
     1381 ggtaaagcgt gccggaatcc gccatcgcaa atcgtatcag tcgagacaca cttatgcctg
     1441 ctggtcgtta acagcaggag ctaacccggc attcattgca aaccagatgg gccatgcaga
     1501 tgcgcaaatg gtgtttcagg tgtacgggaa atggatgtca gaaaataata atgcgcaggt
     1561 aacgctgtta a
//
LOCUS       DAZFRJ010000425         1559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000425 DAZFRJ010000000
VERSION     DAZFRJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.428"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(167..1354)
                     /locus_tag="ACHKUN_005044"
     CDS             complement(167..1354)
                     /locus_tag="ACHKUN_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO6663119.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1354..>1559)
                     /locus_tag="ACHKUN_005045"
     CDS             complement(1354..>1559)
                     /locus_tag="ACHKUN_005045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663120.1"
                     /translation="LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGI
                     SAGGMYCPECGTVHWPDGVPPPF"
BASE COUNT          363 a          479 c          364 g          353 t
ORIGIN      
        1 aaaaagatta aaaaaacacc caaaaacgga tgtttattca acactgcctg cgtgccatga
       61 gtgcaccgcc gtcaattaaa tggatcttat cgattgatac tgcatatcac tgatggtttt
      121 ttaccccatt cccctgaaaa tggacgcaac ttctccgatg cggctctcag ccgcacaccc
      181 gctgttgcgc cagtggctca tgcatcagga ccagctctgt cagacggtag ccccgcttca
      241 gccccgtaaa acgcatctga cctccgcaca ggatgcactt cagcgggtca accttcagta
      301 accgctgata catccctctc cacctgattt gcatcgccgt ctttctcacc gtctccgtta
      361 tgacgtacac aacatcttcc agtaaccggc gcttcaccgg actcaggaaa ccgtagtacc
      421 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccata aactcatcac
      481 cactcatcac caggtattct tcccgttttg tccggtgact gttgtaacgc agaccaattt
      541 catcctgacc agcatagtgc tccagacggc tcatcggcac cggcggtttt ttcaggtaag
      601 agccgaagta caccgccaca tgtgtggcgt tatccatcac ccgggatacg ttgacattcc
      661 agccccgccg gtaatgactg tccaggaagc gattccagtc ccgtttactg cttccttctg
      721 ctgccagcgc atccggtatc accaggtccg gatatttccg tgacagtaac cgcgttatcc
      781 ggtaacgcca catactcatc accttacggg cgtaaaaatg aaggtttttc caggtgtggt
      841 ctgatgtcac gccgccggtc gttgtcgaca ggtgaatgtg cggatgccac tcctggtcac
      901 gtccccatgt gtggatcacc gtgaatatcc ccggcaccac atctgcctgg cggcagattt
      961 cctgtatcac atccgcagca atgcggctca tctctgccag taacctccgg ttgtggaaca
     1021 ccagggacca gtactggcag ggaagtgtga acacaatatg ctgccacgga cagtcgggaa
     1081 ccagactcag cagatactgt atccactgtg cgccagcctt cactccgcag tgcgggcagg
     1141 agcgactttt acaccggaag cagacctttt ttatgtggct gcagtccgga gatgaacagc
     1201 accactgtgt gtaccccatc agcgtggtcc cgcaggccat gatttttgtc acagactcaa
     1261 tgactaccgg acgcactgaa ccttccggtt gtttctccag ccagttaagc cagcggtttc
     1321 cctgctgaaa aatgtcggca aaacggggaa gcatcagaag ggcgggggga ctccgtccgg
     1381 ccagtgaacc gtgccacact ccgggcagta catgccgccg gcgctgatac cggcaagaat
     1441 ggtcgcaaac tcccgctccg tgcagcgggc tatttcagga tacccttcgt catcaacacg
     1501 tacaaaccag aagaccagct ttttgtttcc ggcatccaca aagaagggaa tattcaggt
//
LOCUS       DAZFRJ010000426         1551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000426 DAZFRJ010000000
VERSION     DAZFRJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.429"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..158
                     /locus_tag="ACHKUN_005046"
     CDS             <1..158
                     /locus_tag="ACHKUN_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663121.1"
                     /translation="TVIIIAHRLSTVKNANRIIVMDNGFISEDGTHKELISKKDSLYA
                     YLYQLQA"
     gene            162..>1551
                     /gene="ehxD"
                     /locus_tag="ACHKUN_005047"
     CDS             162..>1551
                     /gene="ehxD"
                     /locus_tag="ACHKUN_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO6663122.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISY"
BASE COUNT          538 a          234 c          331 g          448 t
ORIGIN      
        1 gaaccgtaat tattattgca cacaggttgt caactgtaaa aaatgcaaac agaataattg
       61 ttatggataa tggctttatt tctgaagatg gcacacataa agagcttatc tccaaaaaag
      121 acagtttata tgcatattta tatcagttgc aggcataacg tatgcgcttt tacatgaaag
      181 gtttatggga tttggtttgc cgttataaga cggttttttc tgatgtctgg aaaattcgtc
      241 acacacttga tgctcctgtg agagaaaaag atgagtatgc atttcttcct gctcaccttg
      301 aactcattga aacaccagta tccagacgtt ctcattttgt tgtatggagt attttattat
      361 ttgtaattat atctcttctc ttatctgttc ttgggaaagt tgaagtggtt tcagtagcaa
      421 atggtaagtt tactcatagt ggaagaagca aagaaataaa accgatagag aatgcgattg
      481 tagaaaaaat aatggtaaaa gatggctctt ttgtaaaaaa aaatgatcca ttagttgaat
      541 tgacggtgcc tggtgttgaa tctgatattt taaaatcaga agcatctttg ttgtatgaaa
      601 aaacagaaca atatcgctac gcaattcttt ctgaatcaat acaacggaat gagcttcctg
      661 aaataaggat aacagatttt cctggcggag aagataacgc gggaggtgaa cattttcaga
      721 gggttagctc attaataaaa gagcaattca tgacatggca gaacagaaaa aatcaaaagc
      781 agctcacatt aaataaaaaa atagttgaac gggatgcagc gcttgctcgt gttagcctgt
      841 atgagcatca ggtatcacag gaaggaagaa aactcaatga ttttaagtat ttgttgaata
      901 aaaaagctgt ttctcaacat tcagttatgg agcaggaaaa tagctatatt caggcaaaaa
      961 atgaacatgc agtctggctt gcacaggttt ctcaacttga aaaagaaata gaacttgtgc
     1021 gggaagaact ggcactggag acgaatatct tcagaagtga aattatcgag aagcacagaa
     1081 aatcaacaga taacattgtg ttgctggagc atgagcttga aaaaaacagg cagagaaaag
     1141 catcatcttt tattaaagct cctgtgagtg gtactgttca ggagttaaat atacatacag
     1201 aaggtggtgt tgtcacaaca gcagaaacgc tgatgattat tgtccctgat aatgatattc
     1261 tcgaagtaac agcatctgta cttaacaagg atatcggttt tatacaacct ggacaggagg
     1321 tcgttattaa agtagatgca tatccatata cacgtcatgg ttaccttaca gggaaagtaa
     1381 aaaatataac tgcagattct gtttctgttc cggatacagg gcttgtattt aacgtgatta
     1441 tatctgttga tcggaatgat atacagggag aaagaaaaaa aatccctgtt acagctggaa
     1501 tgactgttat ggcagaaata aaaaccgggg tgcgtagtgt tatcagctat c
//
LOCUS       DAZFRJ010000427         1551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000427 DAZFRJ010000000
VERSION     DAZFRJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.430"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1551
                     /locus_tag="ACHKUN_005048"
     CDS             <1..>1551
                     /locus_tag="ACHKUN_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6663123.1"
                     /translation="VYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTA
                     VIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD
                     RLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRTVKGETAERWQY
                     DERGWLTDISHISEGHRVAVHYGYDSKGRLASEHLTVHHPQTEALLWQHETRHAYNAQ
                     GLAKRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTA
                     YTPAGQLQR"
BASE COUNT          381 a          456 c          470 g          244 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta
      661 tgtaacccgc tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg
      721 cctgagccag taccacgcat acgacagccg tggacagtta actgcggtga aagacacgca
      781 gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg tcattgcccc
      841 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatct gtaccacgca
      901 gggcgggctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag
      961 tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac
     1021 cggctttgac ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag
     1081 cgaggatgag gggctggtca cccactggta ctatgacgaa gcagaccgcc tcactcaccg
     1141 caccgtgaag ggtgaaaccg cagagcggtg gcagtatgac gaacgcggct ggctgacaga
     1201 catcagccat atcagcgaag ggcaccgggt ggcggtgcac tatggttatg acagtaaagg
     1261 ccgcctcgcc agtgaacacc tgacggtgca tcatccgcag acggaagcac tgctctggca
     1321 gcatgagacc agacatgcgt acaacgcgca gggactggcg aaacgctgta taccggacag
     1381 cctgcccgcc gtggaatggc tgacctacgg cagcggttac ctggcaggca tgaaactcgg
     1441 cgacacaccg ctggtggagt acacccgcga ccgcctgcac cgggaaacgc tgcgccgctt
     1501 cggccgttat gaactcacca ccgcttatac ccctgccggg cagttacagc g
//
LOCUS       DAZFRJ010000428         1545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000428 DAZFRJ010000000
VERSION     DAZFRJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.431"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..357)
                     /locus_tag="ACHKUN_005049"
     CDS             complement(<1..357)
                     /locus_tag="ACHKUN_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6663124.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            485..1345
                     /locus_tag="ACHKUN_005050"
     CDS             485..1345
                     /locus_tag="ACHKUN_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6663125.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          420 a          304 c          405 g          416 t
ORIGIN      
        1 cggaaccaga cggacttcat cttttggcgg gcgtccgcgt cgcgcgcgca ctttttcgga
       61 caggatccgc acaaacggtc ttacaccatc cagatcaaat gattccggtg tcagcatccg
      121 ggcttcgcca atacgcattc cggtattcca gagggtggcg aacagcatat ggtgacgctg
      181 atccggcata tagaaaagaa gggcactcac ttccggggcc agcaggtagg ccggtgtggc
      241 ccctgtggaa gtggccattc ttctcaggga gagcgctgtt gcaaaatcca cccccggcgc
      301 aatgggtaac agggagacgc gttctggtga attctgcagg ggaatgacat tgttcatgag
      361 ttatttttca cgtagcacac gcactgttta tggatactga ttgcgctggg cattctggta
      421 ttgtgactgg tatgatgctg gcgctgattc tgatgattaa taaacaacat aacacaggat
      481 atttatgaaa attacggatc acaaactttc tgaaggtatt gctctgacat ttcgtgttcc
      541 tgaaggaaat atgaaacatc ctttgattat cttatgccat ggattttgcg gtatccggaa
      601 tgttctgctc ccttgctttg cgaatgcttt cacagaagca gggttcgcaa cgattacttt
      661 tgattatcgt ggattcggtg agagtgaagg agaacggggc cgtctggttc cagcaatgca
      721 gatcgaagat attatttcag ttatcaattg ggcagaaaag caggcatgta ttgataatca
      781 gcgtatcggg ttatggggaa catcgctggg tggttgtcat gtgttcagtg cagctgcgca
      841 agatcaaaga gtgaaatgta ttgtcagcca gctggctttt gctgatggtg atgctctgat
      901 taccggagag atgaatgaat tggagagagc ttcttttctg tccacactga ataaaatggc
      961 agagaaaaag aaaaacacag ggaaggagat gtttgtgggg gttacacggg tactaaatga
     1021 tgatgagtcg aaggtattct tcgagaaagt caaagctcag catcccgaaa tggatataaa
     1081 aatacctttc cttactgtca tggaaacgtt gcaatataaa ccggccgaga gcgcagccag
     1141 agtccagtgt ccagtattgg ttgttattgc tggccaggat agtgttaatc caccggaaca
     1201 gggaagagca ttatatgatg ctgttgcttc tggtactaaa gaattatatg aggaagctga
     1261 cgcatgccat tatgatattt atgagggcac attttttgag cgggtagcag ctgtgcaaac
     1321 acaatggttt aaaaaatata tttaatagtg agcctgtgtt taccattctg tgtaaatact
     1381 ttttcttaaa agtgaccgtc aaggcggtca ctaaacttta taataaagag gcatcgttca
     1441 tttccgggag gcagactgga ttgccagccc cccacttttt accaagctgc aggtatgggg
     1501 gacacggaca ctggtgttca cagaaaaaaa taaccatctc tgaaa
//
LOCUS       DAZFRJ010000429         1543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000429 DAZFRJ010000000
VERSION     DAZFRJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.432"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..720
                     /locus_tag="ACHKUN_005051"
     CDS             <1..720
                     /locus_tag="ACHKUN_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6663126.1"
                     /translation="GHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVA
                     LGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGK
                     DNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQ
                     PSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYA
                     HSVSGSSAEGYNGQATLNVTF"
     gene            841..>1543
                     /locus_tag="ACHKUN_005052"
     CDS             841..>1543
                     /locus_tag="ACHKUN_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFO6663127.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLT"
BASE COUNT          332 a          424 c          427 g          360 t
ORIGIN      
        1 ggccattctt ccgttgatgt taaggatgat gacggctccc gtgccggcac ggtccgggat
       61 gatgccggca gcctgggcgg atacctgaat ctgatacaca acgcctccgg actgtgggct
      121 gacattgtgg ccctgggaac ccgccacagc atgaaagcgt caacggacaa taacgacttc
      181 cgcgcccggg gttggggctg gctgggttca ctggaaaccg gtctgccctt cagtatcact
      241 gacaacctga tgctggagcc acaactgcag tatacctggc agggactttc cctggatgac
      301 ggtaaggaca acgccggtta tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc
      361 ggtttccgtc tgggcagcca caacgatatg acctttggcg aaggcacctc atcccgtgcc
      421 cccctgcgtg acagtgcaaa acacagtgtg agtgaattac cggtgaactg gtgggtacag
      481 ccttctgtta tccgcacctt cagctcccgg ggagatatgc gtgtggggac ttccactgca
      541 ggcagcggga tgacgttctc tccctcacag aatggcacat cactggacct gcaggccgga
      601 ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccggtta tgcccacagc
      661 gtcagcggca gcagcgctga agggtataac ggtcaggcca cactgaatgt gaccttctga
      721 cagaaccatc gcctctctgt ggtcccggtc atcatgaccg ggacccggac cggcgcaacg
      781 gatcttcaac gccacattcg ctggcattaa caataacatg atattcatca cggagtgact
      841 atgttacaga tagtcggcgc gctgatcctg ctgatcgcag gatttgccat tcttcgcctt
      901 ttgttcagag cattaatcag cacggcttct gcgctggcag ggctcatatt gctgtgtctg
      961 ttcggcccgg ccttactggc tggctatatc accgaacgca taacccggtt gttccatatt
     1021 cgctggctgg caggcgtatt tctgacgatt gccggaatga tcatcagctt catgtgggga
     1081 cttgatggta aacatatcgc gctggaggct cacacctttg actctgtgaa atttattctg
     1141 accaccgctc tcgccggtgg tctgctggct gttcccctgc agatcaaaaa cattcagcag
     1201 aacgggatca caccagaaga tatcagcaag gaaattaacg ggtattactg ctgtttttat
     1261 actgcctttt tccttatggc gtgttctgca tgcgcaccat tgatcgcgtt acagtacgat
     1321 atttcaccgt cactgatgtg gtggggcggg ttgttgtact ggctggctgc attagtgacg
     1381 ctgctatggg cggccagcca gatccaggcg ctgaaaaaac tgacctgtgc catcagccag
     1441 acactggaag aacaaccggt gctcaacagt aaatcgtggc tgaccagttt gcaaaacgat
     1501 tacagccttc ctgactcact gacggagcgc atctggctga cgc
//
LOCUS       DAZFRJ010000430         1534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000430 DAZFRJ010000000
VERSION     DAZFRJ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.433"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1431
                     /locus_tag="ACHKUN_005053"
     CDS             <1..1431
                     /locus_tag="ACHKUN_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6663128.1"
                     /translation="HPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRT
                     DDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMS
                     LSRKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLA
                     EKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQV
                     EPEYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRL
                     PGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYN
                     LYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATAC
                     GHAGIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTE
                     SVGIGAGVDASVMTLACRTWQECWVN"
BASE COUNT          385 a          341 c          476 g          332 t
ORIGIN      
        1 cacccggaca gtaccctcac ggcgtggccg gataaccgca tcgcagagga tgcgcactat
       61 gtttaccact atgatgaata cggcagactg acggagaaga cggaccttat cccggcgggt
      121 gttatccgga cggacgacga gcggacccac cactaccact acgacagcca gcaccgtctg
      181 gtgttctaca cgcggataca gcatggcgag ccactggtcg agagccgcta cctctacgac
      241 ccgctgggac ggcgaatggc gaaacgggta tggcggcggg agcgtgacct gacggggtgg
      301 atgtcgctgt cgcgtaaacc ggagatgacg tggtacgggt gggacggcga ccggctgacg
      361 acggtacaga ccgacaccac gcgtatccag acggtatatc agccggggag cttcgcgccg
      421 ctcatccgca tcgaaacaga caacggcgag cgggagaaag cgcagcgccg cagcctggcg
      481 gagaagctgc agcaggaagg gagcgaggac gggcacggcg tggtatttcc ggctgaactg
      541 gtgcggctgc tggacaggct ggaggaagaa atccgggcag accgcgtgag cagtgaaagc
      601 cgggcgtggc ttgcgcagtg cgggctgacg gtggagcaac tggccagaca ggtggagccg
      661 gaatacacac cggcgcgaaa agttcatttt taccactgcg accaccgggg cctgccgctg
      721 gcgctcatca gcgaagacgg caatacggcg tggcgcgggg agtatgatga atggggcaac
      781 cagcttaatg aagagaaccc gtattacctg caccagccat accgtctgcc ggggcagcag
      841 catgatgagg aatcagggct gtactataac cggaaccggt actatgaccc gctacagggg
      901 aggtatatta cacaagaccc cattgggctg gcggggggat ggaacctgta taattaccca
      961 ctgaatccga taataaggat ggatcctttg ggtttgtata atttatatca attattatat
     1021 gatgtttggc atgatgattc atatggaaca tcatcaattg atattactgg cagtggagat
     1081 ctaatatcat taggtggtca tgcaggactt ggcgttgcgt ttgctaaaaa gaaaggtgaa
     1141 atgttatctg atatttgtat ttatgctaca gcatgcggac atgcaggaat tggtggtggg
     1201 ataaatgcgg ctatcacata ttctgagacc aagtctttac ctgcatcggg agtcagcaat
     1261 tcagtaggtg taacggttgg cggcggagtt ggggggcatt ttgcgtatac ttatgtagtg
     1321 gatgttgata atccagaatc atcgacagaa tctgttggta tcggtgcagg tgttgacgct
     1381 tcagttatga ctctggcttg tagaacgtgg caagaatgct gggtcaatta acaggaggaa
     1441 atatgaaaaa atatatattt ttagttttat ttttattgtg tggttgttct ccagtggaca
     1501 tagagccagc tagacaagaa gttaatatct ttcg
//
LOCUS       DAZFRJ010000431         1527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000431 DAZFRJ010000000
VERSION     DAZFRJ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.434"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..527
                     /locus_tag="ACHKUN_005054"
     CDS             39..527
                     /locus_tag="ACHKUN_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO6663129.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            610..>1527
                     /locus_tag="ACHKUN_005055"
     CDS             610..>1527
                     /locus_tag="ACHKUN_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6663130.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMN"
BASE COUNT          466 a          282 c          336 g          443 t
ORIGIN      
        1 agcttaacta agctatacta cttttattga gagtttaaat gaaaaataaa ttgcttattg
       61 cggctgtgtt gactggtctt ctttcaggat gcgttgatgg caattcaccc tctggcagtc
      121 aggaaggtgt cgtgaaagcg gatcctcgct attcagaatg taacctccct gatgtaaatg
      181 aaagagggcc aattgctgcg ggtctttaca tttccggaac cctcccggaa ggctattggt
      241 taatgaagcc agagcgtaaa atgcattata agggagatgg tatttatcag cttatttatt
      301 ctgaaaaagc aggtgcggta acaatgcaat ttgcgactat gaactggaac ccgcagttca
      361 ctgtaacagg tcttgaattg ccattaaatc aggctaaaac actcaagaaa ggaggcatgg
      421 ataaaaatac aaaagtcgtt attcctgccg atggaaagta tgtttggacg attcaaattg
      481 gtcctgataa aaaggcaata gccgccatgg tagcaccatg taaatagtta tttttagcac
      541 tccactattc aacattcgtt gaatagtgga gtatcagtat tccaaaattc ttgttaatga
      601 gagcagatta tggccgcggt aattctacgc aaagttgaaa aaatttatga caatggattc
      661 aaagctgttc acggcattga tctcgatata aacgatggtg aattcatggt ttttgttgga
      721 ccatctgggt gtgccaaatc aactacatta cgcatgattg caggtcttga agacgtatca
      781 ggtggtgaaa tttacattag cgaccgcctt gttaataccc ttccgcctaa agatcgcagc
      841 attgcaatgg tgttccagaa ctatgcgctg tatccccata agactgtatt tgacaatatg
      901 gcttttggtt taaaaatgca aaaaaggcca aaagaagaga ttaagaaaat tgtcgaagaa
      961 gttgcagaaa aacttgaaat tagcgattta ctttaccgaa aaccgaaaga aatgtcgggt
     1021 ggtcagcgtc agcgtgtcgc ggtaggccgt gcaattgtcc gtaagccaga tgttttctta
     1081 tttgatgagc cactatctaa ccttgatgca aaacttcgtg tttcaatgcg agtcaaaatt
     1141 gctcagttgc atcagtctct caaggatgag ggtcatccag caaccatgat ttatgttaca
     1201 cacgatcaaa ttgaagcatt gaccctcggt gatcgtattt gtgtgcttaa tcaggggcga
     1261 ataatgcagg tcgacacacc agttaatctg tataaccacc ctgctaacag attcgttgct
     1321 ggctttattg gttccccgtc aatgaatttg atagatgcgg ttgtacgtga ggaagacggt
     1381 aaactctata ttgacattgc caaaaatatt cggttgtaca tacctgagaa taaacaatca
     1441 gtactcaaag cgtatataga taaacccgtt tgttttggta ttcgtccaga acatatctct
     1501 ttgagtttga attgcaaaga aatgaat
//
LOCUS       DAZFRJ010000432         1523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000432 DAZFRJ010000000
VERSION     DAZFRJ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.435"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..345
                     /locus_tag="ACHKUN_005056"
     CDS             <1..345
                     /locus_tag="ACHKUN_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6663131.1"
                     /translation="TEMPDFHREDDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILL
                     CCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTG
                     IYNDLAAASACM"
     gene            342..923
                     /locus_tag="ACHKUN_005057"
     CDS             342..923
                     /locus_tag="ACHKUN_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6663132.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            1266..>1523
                     /locus_tag="ACHKUN_005058"
     CDS             1266..>1523
                     /locus_tag="ACHKUN_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663133.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGA"
BASE COUNT          355 a          371 c          468 g          329 t
ORIGIN      
        1 actgaaatgc cggattttca tcgcgaggat gactggtggc gcaacggtca gaacctttac
       61 ctggacaata tggcggtcac cggcttttac cgggtgcccc tgtcctctgc acaggcgggc
      121 gatattctgc tgtgctgctt tggtgcttcg gtaccgaacc atgccgccat ttactgcggc
      181 aacggtgagc tgcttcacca tctgcctgaa caactgagta aacgggagag gtattccgaa
      241 aaatggcaac gacgaacgca ttctgtctgg cgtcaccgcc actggcacgc atctgccttc
      301 acggggattt acaacgattt ggccgccgcc tcagcctgta tgtgaacacg gcagcggaag
      361 ccatccgggc gctgtcgtta caggtgccgg gctttcgccg tcagatgaac gaaggctggt
      421 accagatacg tattgccggt tatgacacgg caccggaggc ggtgtacgcc cgtcttcacg
      481 aacagctggg tgagggaacg gtcatccata ttgtgccgcg actggccggg gccggaaagg
      541 gtggactgca gattgtgctg ggggcggcag ccatcgtggg ctctttcttc actgccgggg
      601 catcaatggc gttatggggt tcagccctgg cagccggtgg tttttctgcc accacaatgc
      661 tgttttcact tggagccagc atgattctgg gcggtgtggc ccagatgctg gccccgaagg
      721 caaaaacacc ggattaccgc gcaacggata acggcagaca gaacacgtac ttttcctcgc
      781 tggataacat gattgcccag gggaacccga tgccggtgcc ttacggggaa atgctggttg
      841 gctcccgccg tatatcccag gacatcagca cccgtgatga aggcgggggc ggaacggtcg
      901 tggttgtcgg gcgacaggga taaaacataa aaaaatcccg cagtgatcgc ggagctgcgg
      961 ggacagacaa atgaagatca atgttaagga gttgtttttg ttactcgggc aaaaaaacac
     1021 taacgcagcg aaattataag cgccacagtc agtgtgtgaa aatgtgaaga tattcagaat
     1081 ttttatgcca ttaccggttt taaccaacag gattatcggt gggcatgaaa gaaaaccccg
     1141 gtatctgctg ataccggggt ttctctttag catggcagaa atgtgtttca tgcttttcgg
     1201 gcgaaggata tccgacttct gtacggaatg gcaagtggcg gttaatttat tcaggggaag
     1261 gctgtatggg aaaaggtggc ggtaaggcac acacgcctcg tgaggcgaag gataatctca
     1321 aatccacgca gatgatgagt gtgattgatg cgattggtga gggaccgata gaaggtccgg
     1381 tgaagggact gcagagtatt ctggtgaaca aaaccccact gacggacacg gacggcaatc
     1441 ccgtgataca cggtgtgacc gcggtctggc gtgccgggga gcaggagcag acaccaccgg
     1501 aaggctttga gtcctccgga gct
//
LOCUS       DAZFRJ010000433         1488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000433 DAZFRJ010000000
VERSION     DAZFRJ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.436"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..199)
                     /locus_tag="ACHKUN_005059"
     CDS             complement(<1..199)
                     /locus_tag="ACHKUN_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO6663134.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDAD"
     gene            complement(207..602)
                     /locus_tag="ACHKUN_005060"
     CDS             complement(207..602)
                     /locus_tag="ACHKUN_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6663135.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(599..1177)
                     /locus_tag="ACHKUN_005061"
     CDS             complement(599..1177)
                     /locus_tag="ACHKUN_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663136.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1189..>1488)
                     /locus_tag="ACHKUN_005062"
     CDS             complement(1189..>1488)
                     /locus_tag="ACHKUN_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6663137.1"
                     /translation="IRGYMGTSATMTSGEQSGAVIRGVFDDPENISYAGQGVRVEGSS
                     PSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCHLWLGRGVPPAVNRRR"
BASE COUNT          318 a          470 c          355 g          345 t
ORIGIN      
        1 agtccgcgtc ttcatcatcc aggtagttat catcgtaggg ttctgccgtc atctcgcccg
       61 gcgtcagatc cttcacctta gccagtcgct gccagtcatc gtctgacaac gggtttgcat
      121 aagcatcacc cttgccgttg taaacccaca gagtggtacc ggcacctttt accggctcaa
      181 ggggatttgg tgttgccata tcgtcctcac atctcgtatg taatggaata agtcagatct
      241 gcagaactcc ataacgccat atcgtcatca cgacgatact catagccctg cgtaaccatc
      301 gtggtaatca gtcctgccag tgccgggata tcgctcatca ccggataaat ccgggactcc
      361 atccacgcat ccagctctga atccggcacc tgagcaggca ggaaaacttc aatatgcagc
      421 tccgcctgcc aggtatcgct gtccagctct tcgcccgtgt attcagcgcc ggtgagataa
      481 acggcaactg ccggaaaatc cgcctcatca aaaacagcgg ggcgaccatc aaaaagcgtc
      541 gccccggtgt catgcttctc cagtgcatcc agtacggctg cacggagttc agtatgtttc
      601 atcgctttat taccattctc agttgatgct gcagcgcata gcccagctct ttcggaagac
      661 gttcacgccg tatccgttca atattctgtt taaacgccgt ggtaagcggc accgccatcg
      721 ggattttcac cacatcaatg gggtaacggt ttttcccggc cacacgctgc atgacatgcc
      781 accgcccatt tttcagttgc tgaataaacg cgccgggaat acgacggtta cccaccacaa
      841 gcacgctgcc gccacctttc agggatgaac gctgcccctt tttacgacgc ctgcgtcggg
      901 acaggacaac ccgcgcattc cccagcttga ttacgggcaa atccccccgg ttaaccctga
      961 ttctggcctg cggattttta accgtggccc ttttcagcct ggccctttcc tttaccagtt
     1021 tccggcgtac ctttgtctca cgggcaacct gtgacgcaga ctgcgatatc gcggatgaag
     1081 caacgcggtt aatggccatt gctgacgcac cgggcaccgc cgttttgctg atacggctga
     1141 ggttttcaac ggcctgctca agacctttta tggccataca tccccctttc agcggcgacg
     1201 gttaacggca ggcggcacgc cacgcccaag ccagagatga cagcttccgc catcatccgg
     1261 cgaaatccgg tctatccaga agttttcctc accgatggtc agcgtgtctc cacgccgcag
     1321 ctgccgcaca tcatcagtcc ggacaaacag ggacgggctg gagccttcaa cgcgcacgcc
     1381 ctgtccggca tagctgatat tttcagggtc atcaaaaaca ccacgtatta ctgcgccgga
     1441 ctgctcaccg gatgtcatgg tggctgacgt tcccatgtac ccgcgtat
//
LOCUS       DAZFRJ010000434         1483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000434 DAZFRJ010000000
VERSION     DAZFRJ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.437"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHKUN_005063"
     CDS             complement(<1..153)
                     /locus_tag="ACHKUN_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6663138.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>1483)
                     /locus_tag="ACHKUN_005064"
     CDS             complement(143..>1483)
                     /locus_tag="ACHKUN_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6663139.1"
                     /translation="ISYRPNWRWLGMRETAAKSFVDEVEAAWSEYAEGMSGEIDVEGK
                     RTFTEFIREGVGVHAFNGEIFVQPVWDTESTQLFRTRFKAVSPKRVDTPGHGMGNRFL
                     RAGVEVDRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPAMLHIFEPVEDGQTRG
                     ANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAGAPQGQKDN
                     PLINILDKFSTWYDTNNVTLGGVKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIA
                     AGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIASRLATQMFSCWLEEALL
                     RGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELA
                     LMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          336 a          471 c          350 g          326 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcactttcaa
      901 tcgtcgctgc atacatggct ttcacaatgg ccgactgaag ctgtgttgcc tgcagggaat
      961 ccagcatctt cagccgttcc atgacgctgt aaaactgatt agccccacgg gtctgcccgt
     1021 cctccaccgg ctcgaaaata tgcagcatgg ccggacgccc ggtgggtagt tcacgcggga
     1081 tccgttccca tcgtccacta ccagagaacg gaaaatcatc ctcacagata tggtacgcaa
     1141 cggcacggcc atatcgatcg acctccaccc cggcccgcag aaaacggttc cccataccgt
     1201 gtcctggcgt gtccacccgt ttcggactca cggctttaaa acgcgtacga aacagttgcg
     1261 tgctctccgt atcccagacc ggctgcacaa agatttcgcc gttaaacgca tgaacgccca
     1321 caccttcacg gataaattcc gtaaacgtgc gtttcccttc cacgtcgatc tcgccagaca
     1381 tcccttcggc gtattccgac caggccgcct ccacctcatc gacaaaactt tttgctgcag
     1441 tctcccgcat ccccagccag cgccagttcg gacggtagct gat
//
LOCUS       DAZFRJ010000435         1480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000435 DAZFRJ010000000
VERSION     DAZFRJ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.438"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1480
                     /gene="espL1"
                     /locus_tag="ACHKUN_005065"
                     /pseudo
     CDS             1..1480
                     /gene="espL1"
                     /locus_tag="ACHKUN_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT          455 a          316 c          283 g          426 t
ORIGIN      
        1 cttattactg gaaaccacgc ctattaaagc taaccatttg cgaagcatac tggattatat
       61 tgaacaggaa cagttaactg ctgaagatcg taaccattgt atgaaactgt ctaaaaaaat
      121 ccatagagaa aaaactatac acccaacagt aaatctcaat ggtagtgcat gttttttgca
      181 atctccttct gacgctattt tttgtcgcca tctgtcattg caatacgccc ttgattcatt
      241 gagaaatgga aaaggcaaag tcaacctgat taaacattac tcctccgttg aatccataca
      301 gcagcatgtc cccttagtcc gggacgcgga gttcagagca ttacttcgcc atcctcctgc
      361 agggagtcgc gttatcgcga gtaaggattt tggcttcgct ttagattttt tcttttgtcg
      421 aatgatggca aacaatatca gtcatatgtc cgcgatttta tatatagaca atcatacttt
      481 gtcagtaagg ctacgaataa agcagtcagt gtatggacaa ttaaactatg ttgtgtccgt
      541 ttacgacccg aacgatacca acgttgccgt cagagacacc cacaggacag cacggggctt
      601 tctttcgctt gataagttca tcagttcagg tcccgatgct cagacctggg ctgataggta
      661 tgttcgcaac tgtgcaattg ctattctgcc cctattacct gtgggagttc caggggctat
      721 tttcgcgggt attgcatcac gaatgccatt tgcccctata catccatcgg caatgttgtt
      781 aataatggct acaggccaga ctcaacagct tattacatta ttcaaacagt tgcccatact
      841 ccctgaaaaa gaaatcattg aaataataac tgcgcagaat agcgttggta cacctgcttt
      901 atttttggct atgatgaacg gacatactga caacgtgaaa atatttatgc aagaaattca
      961 gtcactggta gataatcaca tcattcatga agataacctg gttaaattac tgcaaactaa
     1021 aagtgctaac gaaacacctg gactctatat ctccatgttg tatggattcg atgaaataat
     1081 cgatatcttt ctgaatgcat taaccactcc aatagcacaa gaacttttaa acaaaaaaat
     1141 ggtgatggat attttagcca tgaaaacacg tgatggtgaa ccagggttat ttgctgcaat
     1201 ggaaaataat caccctttgt gtgtcacacg gttcctctct aaagtttatg ggatcgccgt
     1261 taaatataaa ctcagcaaaa tcaacatcat ggacttatta aaaggcgcga cagcacaggg
     1321 aacccctgca ttatacatcg ccatgagcaa gggtaatgaa gacgtcgtgt tatcttatat
     1381 atcgacgctg ggtgcttttg caaaaaaaac attcttttag tcaacatcag ttatttacac
     1441 tattggccgc taaaaatcat gacaacatgt cagctgttca
//
LOCUS       DAZFRJ010000436         1479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000436 DAZFRJ010000000
VERSION     DAZFRJ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.439"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            858..1460
                     /gene="fimB"
                     /locus_tag="ACHKUN_005066"
     CDS             858..1460
                     /gene="fimB"
                     /locus_tag="ACHKUN_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO6663140.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          445 a          263 c          326 g          445 t
ORIGIN      
        1 taagtaattt atttagagta taaacaagga aagatttgca agataactca gtgggcgtgg
       61 tgaaaataag tgtttacccg ccatcaggct gagcataatt ctcatcatga aatatgtttc
      121 ctggtttgtg gcttgtagct ggtcactcct gaagtctctc tggagaggct tgttgatgtt
      181 ggtgttttca ggatgatgtt tcacttagct tgtttgccga atagcccggc gaacagctgt
      241 gattgaggaa ggtttaagtc gcagtgacca aagccatatt taccaacgga tgcagatgaa
      301 aaaatcatct cctgctttcc cccatatctc caggataaaa aggaatgtaa caatctcatg
      361 aactaaactg acgaaccagc caggaataat cgctagggac ctaagaatta gcctgataat
      421 agctactaag aaattactgc gttccatgaa atagccattt tgtggcaaat ggagttgtct
      481 aataatgtca tatgtgagac ggatagttga acgaatatta aattttgctg aattttttat
      541 gttgatttta cttgttacag aacatatcac atgatatata gataagatta gttgcattaa
      601 tgatgagggt tattattaga ttcgtatccg attgataaat atataaaggt acatagcatg
      661 caagagcata gcgtttgtat ggcaacgtta ttataagtaa ctgttgctac tccatttaag
      721 ttcactcaga agaactggtc cacttacgtt agttattaag caaacgttcg cttttataaa
      781 cataatcagg ataaaaatgt tggattattg ctaacccagc acagctagtg cgcgtctgta
      841 attataaggg aaaaacgatg aagaataagg ctgataacaa aaaaaggaac ttcctgaccc
      901 atagtgaaat cgaatcactc cttaaagcag caaataccgg gcctcatgca gcacgtaatt
      961 attgtctgac tttgctttgt tttattcatg gtttccgggc gagtgaaatt tgtcgattga
     1021 ggatttcgga tattgatctt aaggcaaagt gtatatatat ccatcgatta aaaaaaggct
     1081 tttcaacaac gcacccgcta ttgaacaaag aagttcaggc tttaaaaaac tggttgagta
     1141 tccgtacttc gtacccgcat gctgagagcg aatgggtatt tttatcgcgt aagggaaatc
     1201 cgctttctcg gcaacagttt taccatatta tctcgacttc cggtggtaat gccgggttgt
     1261 cactggagat tcatccgcac atgttacgcc attcgtgtgg ttttgctttg gcgaatatgg
     1321 gaatagatac gcgacttatc caggattatc ttgggcatcg caatattcgt catactgtct
     1381 ggtataccgc cagcaatgca gggcgttttt acggcatctg ggatagagcc agaggacgac
     1441 agcgtcacgc tgttttatag agatagttga gataccagg
//
LOCUS       DAZFRJ010000437         1473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000437 DAZFRJ010000000
VERSION     DAZFRJ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.440"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..>1473
                     /locus_tag="ACHKUN_005067"
     CDS             62..>1473
                     /locus_tag="ACHKUN_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO6663141.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSSSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEA"
BASE COUNT          350 a          352 c          466 g          305 t
ORIGIN      
        1 tttttatgtg ttacatgatt gtgtattccg gataattact ttgctggcgg caggagaggt
       61 tatgacattt aaacactacg atgtggtcag ggcggcatcg ccgtcagacc ttgctgaacg
      121 actgacacaa aaactgaagg agggctggca gccatttggc agtccggtgg ccatcacgcc
      181 ttataccctg atgcaggcca ttgcggcgga aggtgatgtc accacaccag tggcggtgac
      241 cggtaatgag ggtaaggcgg tggctgtcag tgccaccagc gacccggagt attactttgt
      301 tgtggttctg gcagggcagt caaatggcat gtcgtatggt gaaggtcttc cgctgccgga
      361 gacatatgac cgtccggacc cgcgtattaa gcagctggcg cgccgcagta cggtgacacc
      421 gggcggtgca gcatgcaaat ataacgacat cattccggcg gaccattgtc tgcatgatgt
      481 gcaggacatg agccgtctta accatccgaa agcggacctg tcaaaagggc agtacggtac
      541 cgtggggcag gggctgcata tcgccaaaaa actgctgccg tttataccgg cgaatgcggg
      601 cattctgctg gttccgtgct gtcgtggtgg ttcagcgttc accaccggag ctgatggcac
      661 atacagtgac gcgagtggtg cctcggagaa ttcaacccgc tggggtgtgg acaagccgct
      721 gtataaggac cttatcggtc gaacaaaagc agcactgaag aagaacccga aaaatgtgct
      781 gtttgccgtg gtgtggatgc agggggaatt tgattttgac ggcacgcccg gaaatcacgc
      841 agcacagttt ggtgcgctgg ttgataaatt ccgtgcagac ctgacggata tggcaggtca
      901 gtgcgttggt ggctctgctg gcggtgttcc ctggatctgt ggagatacga cgtatttctg
      961 gaagcagaag aacgaatcct cgtaccagac ggtgtacggc agctacaaaa acaaaacgga
     1021 aaagaatatc catttcgtac cgttcatgac cgatgagaac ggggtgaatg tgccgacgaa
     1081 caaaccggaa gaagacccgg acattccggg tatcgggtat tacggttcga aatggcgtga
     1141 cagctcagcc acctggacgt cacaggacag ggcgagccat ttcagttcat gggctcgccg
     1201 cgggattatt tccgaccgtc tggcaacggc gattttgcgc catgcgggaa gagtggcgct
     1261 aaacgcgggg gcatcatcga cagtatcaga ggtgcgcccg tcatcgtctt ccggtgcaga
     1321 agccacaggc gtcacaacac tgctctctta ccttgccagc gagtcagagg gaagcctgaa
     1381 agtacaggga tggtcagcca gtggcggcag ggcagaagtg gtcagcgatg cggagggaac
     1441 cggaggtaag gcagtgaagc tgaccaagga agc
//
LOCUS       DAZFRJ010000438         1466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000438 DAZFRJ010000000
VERSION     DAZFRJ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.441"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..383
                     /locus_tag="ACHKUN_005068"
     CDS             <1..383
                     /locus_tag="ACHKUN_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6663142.1"
                     /translation="LKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWV
                     EIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVL
                     GVIADWPVNRVSELLPWRIALPAE"
     gene            418..1374
                     /locus_tag="ACHKUN_005069"
                     /pseudo
     CDS             418..1374
                     /locus_tag="ACHKUN_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          388 a          314 c          375 g          389 t
ORIGIN      
        1 tgttgaaatc cctggaaagc tggttgcgtg aaaagatgaa gaccctgtcg cgacactcag
       61 agttggcgaa ggcgttcgcg tacgcactta accagtggcc ggcactgacg tactatgcga
      121 acgatggctg ggtggaaatc gacaacaaca tcgctgaaaa tgccctgcgg gcggtcagtc
      181 tgggtcgtaa aaacttcctg ttcttcggct ctgatcatgg tggtgagcgg ggagcgctac
      241 tgtacagcct gatcgggacg tgcaaactga atgacgtgga tccagaaagc taccttcgcc
      301 atgtgcttgg cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc
      361 gcatagcact gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac
      421 cgttcagtgg gtattatgtc agatgaagcc ggtattatat ttgatggata taccctgtct
      481 gagttgcctt ttattaacaa aatgtgggat ggctctgtat tgtctgtgga cagaaagaat
      541 gaacccgaac agatgattga aaatgccaga atgacgttat ccctcatggt gcaacccggt
      601 ctttttgacc gatatatgga gcgaaaaggt tctgttgcca gggacagtgg ttttcttgcc
      661 cgttgtctta tcagtaaacc tgcaaccacg caggggaaac gctttattaa cggagctgtg
      721 attccgggag gatcattaac ggcatttcat gaacgattaa tggaactggc cagaggcagt
      781 attgaaaaat ccagtgagga tgaacggtat tgtctgcatt tttcgcctga agcgcaaaaa
      841 atatttattg agcactataa tgttctggag caggatttat ccccttccgg acccttaagc
      901 ccgtttcggg gacatgtttc aaaaaaaaca gaaaacattg caagaattgc agcgttattt
      961 cagtatttca gctatggtga agggaaaatc tctgccgata tcatgacgtc tgctgtcgta
     1021 ataagttcgt ggtacacaga tgaatataaa aaattatttg cgttacctga tgaatctgaa
     1081 ctgcagcaga aggacgccga ggagctgttc gactggctca ttgaggagtg ccgcggagaa
     1141 tgcccacccc gtgtcaggaa aaattatatt ctccagtgtg gcccgggccg gttcagaaac
     1201 aggaaaaaac tgaacgcatt gctgaacatt cttgaatctc agttcaggct ttctgtcgtg
     1261 ccggaaggaa aaaccatgta tgttctgctt ccccagatag ccagtttaaa gttgtctgat
     1321 gtctctggta tttttacctc gggttatcac tacaataagc tgagagcgaa gtaaccggtg
     1381 agcagttttt ctccattatg cttcagactt agatgattga ttattatggg gatttcatca
     1441 ccgatattca gatgtatgaa tatcgg
//
LOCUS       DAZFRJ010000439         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000439 DAZFRJ010000000
VERSION     DAZFRJ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.442"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHKUN_005070"
     CDS             complement(<1..270)
                     /locus_tag="ACHKUN_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663143.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHKUN_005071"
     CDS             complement(365..571)
                     /locus_tag="ACHKUN_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6663144.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHKUN_005072"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHKUN_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          379 a          340 c          413 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tagtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agca
//
LOCUS       DAZFRJ010000440         1460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000440 DAZFRJ010000000
VERSION     DAZFRJ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.443"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..339
                     /locus_tag="ACHKUN_005073"
     CDS             58..339
                     /locus_tag="ACHKUN_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6663145.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            305..1381
                     /locus_tag="ACHKUN_005074"
     CDS             305..1381
                     /locus_tag="ACHKUN_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6663146.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSLHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT          505 a          291 c          325 g          339 t
ORIGIN      
        1 ccacacccgc cagtattctg tatatttact ggcggtcata tcgtaagagg tatggcaatg
       61 aatcttgtga cactcaaaac gtggggaaaa ctcagatatc cggataaccc accatcaata
      121 tcaacgctga gacgatgggc aaggaatgga aacatttatc ctgcacctga actacacggg
      181 aggagttaca gggtggttcc ggaagctttc tatatcaacc caaataaggt tgataccgat
      241 ataacacacc atcagcctaa tgggcgacaa gggagagaca gtccgttact ggagaagtta
      301 aaacatgcag cggaaaaaat acgatcccaa tttacctaaa aacttaacat atcgaaggag
      361 ggacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac taggtaaaat
      421 ttcaagaaga gatgcagtag cgcaggcaat tgaagcaaat cattatatat acaaaaacta
      481 ctctcctgct gccttaattg aaaaactaaa agggttcgac tcatttacta tggcagactg
      541 gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata cttataaaat
      601 tcgggtaaat caactggaga caataaaaga aaaattagga ggggttttac tgacagaaat
      661 aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga aaaacacgat
      721 ggcaggatca atgcgttctg tgttatctga tatgttccgc gaggccattg ttgaaggacg
      781 tatatctcaa aatccagtaa cgccaacaag agcaccgaaa atagtagtta caagagaacg
      841 gctgaaacta aagacataca actgcatcag ggaggcagca gatcaacttc cgacatggtt
      901 cccattagct atggacttag cccttgtaac aggacaacgt cgcgaagaca taacaaatat
      961 gcggttcagt gagatttatg atgatcgtct ccacatcagg caaattaaga caggaatgat
     1021 gattgctatc cccctgtcac tcagccttcc tgtcgctggt ttacgacttg gtacagtagt
     1081 tgaacagtgc cgcatggtaa gtagggggga ttatctaatc agtgccggga ttagaaaaaa
     1141 cagccctgac ggcagccttc acccggacgg cctgacaaag aaatttgtcg cagccagaaa
     1201 attaacaggt atccagttca gtgaaaaccc accaactttt cacgagatca gaagcctggc
     1261 tggacgattg tacaaagaaa catgtgggga agaatttgct caacgtctac ttggccacac
     1321 atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact tactgctctg
     1381 attttaacgt aaatggattg ttaaatgtgt tttggttgtg atataaccaa aaaagaccgg
     1441 aatacagaaa ttcgagtaaa
//
LOCUS       DAZFRJ010000441         1431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000441 DAZFRJ010000000
VERSION     DAZFRJ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.444"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHKUN_005075"
     CDS             complement(<1..677)
                     /locus_tag="ACHKUN_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6663147.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKUN_005076"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHKUN_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1174..1431)
                     /locus_tag="ACHKUN_005077"
                     /pseudo
     CDS             complement(1174..1431)
                     /locus_tag="ACHKUN_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          368 a          348 c          322 g          393 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagtgga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      241 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
      661 gcctgtagtc gttccacagg tcaaattata ttttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
      781 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattagct atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cagataaacc aggccaagcg tatgctggtt
     1201 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     1261 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     1321 cgcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     1381 ttcactatgc acaattttta aaaaccaagg gttagcaata taataacccc a
//
LOCUS       DAZFRJ010000442         1407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000442 DAZFRJ010000000
VERSION     DAZFRJ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.445"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..551
                     /locus_tag="ACHKUN_005078"
                     /pseudo
     CDS             3..551
                     /locus_tag="ACHKUN_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            643..1133
                     /locus_tag="ACHKUN_005079"
                     /pseudo
     CDS             643..1133
                     /locus_tag="ACHKUN_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1133..1282
                     /locus_tag="ACHKUN_005080"
     CDS             1133..1282
                     /locus_tag="ACHKUN_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6663148.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
BASE COUNT          429 a          310 c          321 g          347 t
ORIGIN      
        1 ctataagcgc cagcgtatca attccggcag aacaatctca atccatctcc acaattcctg
       61 gagggagaaa aatagagaca gtgccagaga aagaaggatt gcttgtgttt ttaggaacaa
      121 aatccgtagg tgaatatgct cttaatattc ttggtcaaaa tgtttcaaga gtcacaacag
      181 ggaaaaaacc ttatgatata ttattcctgc acgaagctac aaaacaagat tttgataaaa
      241 agaaaacaga gtttaccttt cctggtgcaa acagaagtta tctacaatca agtaacaccg
      301 atgttgctgc cgcagcagct ataagtattg cggcaacaga gatgaaaact atcctaccaa
      361 aggatttaac accagaaaaa tacaataaaa tttatctgcc aggggatggt tctgctggcc
      421 tacctcttct taagtgtgga gatgaatttt tatcaccaac tgatatcgtc aatcgtctgg
      481 tagaacataa tcttcatgaa gtagaagaca tcagattaac atcctgtcac tcagccaaca
      541 taacaaaaaa ctagactggc cccctgaatc tccagacaac cagtatcact taaataagtg
      601 atagtcttaa tactagtttt tagactagtc attggagaac agatgattga tgtcttaggg
      661 cctgagaaac gcagacggcg taccacacag gaaaagatcg caattgttca gcagagcttt
      721 gaaccgggga tgacggtctc cctcgttgcc cggcaacatg gtgtagcagc cagccagtta
      781 tttctctggc gtaagcaata ccaggaagga agtcttactg ctgtcgccgc cggagaacag
      841 gttgttcctg cctctgaact tgctgccgcc atgaagcaga ttaaagaact ccagcgcctg
      901 ctcggcaaga aaacgatgga aaatgaactc ctcaaagaag ccgttgaata tggacgggca
      961 aaaaagtgga tagcgcacgc gcccttattg cccggggatg gggagtaagc ttagtcagcc
     1021 gttgtctccg ggtgtcgcgt gcgcagttgt acgtcattct cagacgaacc gatgactgga
     1081 tggatggccg ccgcagtcgt cacactgatg atacggatgt gcttctccgt atatcctcat
     1141 tgcgtggctg caggacaata tcgactgcga atcgggaatt atctttgaca acgatgagga
     1201 taaaacggat tcggcagcac tgctgccctg tattgaacag gccagggaag ccaggtactt
     1261 tccttctgtg ctctcagcct gatcccgagt gtgattccct ttcgatatca aaagggactg
     1321 cgggtataat tgagggtgca tttttaatca aaccaaacaa attgcattaa tatcaatatg
     1381 ataaaggaaa ttttcgattc ccgagtc
//
LOCUS       DAZFRJ010000443         1404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000443 DAZFRJ010000000
VERSION     DAZFRJ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.446"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..836)
                     /locus_tag="ACHKUN_005081"
     CDS             complement(<1..836)
                     /locus_tag="ACHKUN_005081"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6663149.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VAS"
     gene            complement(900..>1404)
                     /locus_tag="ACHKUN_005082"
     CDS             complement(900..>1404)
                     /locus_tag="ACHKUN_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO6663150.1"
                     /translation="HETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRI
                     HEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVL
                     VHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKK
                     EYAVFFI"
BASE COUNT          284 a          456 c          358 g          306 t
ORIGIN      
        1 gaagccaccg cggccacatt cccgttaccg gcctgtttca ccggctgcgc cgtggcggcc
       61 ttaccggctt ccagttcacg caggcgcttc aggtgcgcat ccacctgacg gatttccgct
      121 gcggtgttgt cgtaatgctc ttcctcctcc acatccagcg tgcgcccttc ctctgcggct
      181 ttggtcatga cctcctcaag ggaggctgcc agcgctgcac gcttgttttc aaaactttta
      241 atctgttcgc caatattcat tatggtcttt tccttatgaa aaacggttgt tgactgtgcc
      301 gcagcgccgg cagaagatgc gattttcacc accggtttcc ggttgccgga cgcggcagaa
      361 aacgggcggt cgtaagattt aatggtccgg atggtgcatt ccgcattcgc gggcacggtg
      421 acggcagaca cctccatcag ttcccagcgc agaaaatgca gtccgcctcc gtccagaaag
      481 gtgtattcat ggggacggaa gcccacggac agccccctga ccagcccggt cttaatggcc
      541 gcccagacct catccagccg ggcagccagt tgcgacggca tatccggtac gggcttcacc
      601 agtgttgccg tgatttccag cccttcgctg acccggcgca ccgtacactg ccccaccggg
      661 cgggaatggt catgctgcca gagaaacggt atcgcactgc caaactccgc gccctccggc
      721 tccaggatgt caccatcccg atccggagaa ggcgttgacg caatcccggt gatcacccgt
      781 tcatcctcac tgaaggattt caccgtcagc agggaacagg cccgtttaag agtcacatca
      841 gcctcctgaa aataaaaaaa ccgccggagc ggttcgtgat ggttacagtg tgaacagggt
      901 tatatgaaaa aaaccgcata ttctttcttt ttcggttccg ggttaaggga catcagggag
      961 accgcattga acagcgccat cagcgggtca atttttcccc gtccactggc ctgtttggtg
     1021 ataagaatgg cgttaccttt aggctccacc cgggcattgc cgacacacca ggccatcagg
     1081 ggctggtcac catgcaccag caccccttca gccagtttgc gctcggtggt tttaatggcc
     1141 ccgcccagtt tccagccctg gcttatcccc accacaattc cgtcggggat cccggcttcc
     1201 gccagtgaat ccagaatctg ccccacccct gacgggtcaa taccgatatg gtccagtaac
     1261 tcagcctcat gaatgcgacg cacatattcc gccacttccg ccgtgtcatc cccgacacgc
     1321 cggacaatgg tcatatctcc acaggcaaca agatcctgaa accgggacgc ctcgctcttc
     1381 cgtcggacca ccgcggtttc atgc
//
LOCUS       DAZFRJ010000444         1398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000444 DAZFRJ010000000
VERSION     DAZFRJ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.447"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..278)
                     /locus_tag="ACHKUN_005083"
     CDS             complement(<1..278)
                     /locus_tag="ACHKUN_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO6663151.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
     gene            complement(448..524)
                     /locus_tag="ACHKUN_005084"
     tRNA            complement(448..524)
                     /locus_tag="ACHKUN_005084"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(488..490),aa:Arg,seq:tct)
     gene            complement(538..614)
                     /locus_tag="ACHKUN_005085"
     tRNA            complement(538..614)
                     /locus_tag="ACHKUN_005085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(578..580),aa:Arg,seq:tcg)
     gene            complement(624..699)
                     /locus_tag="ACHKUN_005086"
     tRNA            complement(624..699)
                     /locus_tag="ACHKUN_005086"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(664..666),aa:Ile,seq:cat)
     gene            complement(728..1396)
                     /locus_tag="ACHKUN_005087"
     CDS             complement(728..1396)
                     /locus_tag="ACHKUN_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6663152.1"
                     /translation="MFLEKDTLSLKPGSVVLATKCIRELFLMHYGKVKIVDISESVVS
                     QYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEATDMFLMMACLSLFE
                     TDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKLKNEGGM
                     FSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQ
                     GLDY"
BASE COUNT          377 a          360 c          315 g          346 t
ORIGIN      
        1 atctcgcgta acgcggcacg acgaccaatt ttaaaattgg tatccagctg accgataata
       61 gttgcgctaa tcgcattaat ttccgaggta aacgcagaca acgcaggctg attacgtaag
      121 acctgttcga tcttcgcttt ctcggatgcc tcgcattcac catctgcata ggccaccagg
      181 tatgcagcgt taatcaccgc ctgtgccaga tcgcgtttct caaacttttt aatttccgct
      241 gccgctctgc gggttttctt tttgaagatt ccaaacatcg tgacgttcct ttgggtgggt
      301 gagccaacgc ccgggagcga tctgcccaca gagaaagtca cactgaccac tccgtaagct
      361 cacccccgaa aggctctgtg gttgatatgc gccgggcgtg gcgcagatac aaaaaaggcc
      421 cgccgaagcg agcctggaaa ataagtgtgg cgcgttgtag tggagtctaa ccactgaccg
      481 attgcttaga aggcaattgc tctgtccgac tgagctaaca acgcatgatg ctgataatgg
      541 accgccatcg gggacttgac ccccgcgcag ccagcttcga aggctggcgc tctgtcccga
      601 tgagctaata gcggtatgta atatggtggc ccttgctgga tttgaaccag cgacctggcg
      661 attatgagtc gctcgctctc accactgagc taaagggccg agccaaaaaa taataatcag
      721 atgaaaatca ataatcaagc ccttgcctgg atacatatct gtctggcggg aagccataat
      781 agcggtgaaa tacagaaata aagtaggacc tgcttgaata accgcatttt tctgctatag
      841 cctgtccata tccatgccgg gaacataaca tatttacagc aacacgcatc cgctcttcca
      901 gcaacaagcg actgaacatg cccccttcat ttttcagttt tgtctttaac aaactctcac
      961 tcatatgcaa ctgtagagca atcgcaccaa gcgtccagct tgctgatata tctgtctgaa
     1021 ttatcgccct gactttggca cttatgctgg ataaacatcc acttaaaaac aatgacatcc
     1081 gttcatctgt ttcaaacaga gacaggcagg ccatcataag aaacatatcc gtggcctctc
     1141 cggaaaatcc ctggctggta attaaagccg cagccaacgc aggattgttg ggttccagca
     1201 acaggtaaag cggaatgtca gtcagacgag tcctcgtcag cttatgctga ctttccagat
     1261 attgacttac gacggattcg cttatatcga caattttaac tttgccataa tgcataagga
     1321 aaagctccct gatgcatttg gtggccagaa cgactgagcc tggcttaagt gacaacgtat
     1381 ccttttcaag aaaaatat
//
LOCUS       DAZFRJ010000445         1378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000445 DAZFRJ010000000
VERSION     DAZFRJ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.448"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1378
                     /locus_tag="ACHKUN_005088"
     CDS             <1..>1378
                     /locus_tag="ACHKUN_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6663153.1"
                     /translation="FKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKPSPDLLCG
                     YLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPGINPYINF
                     DWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKINACIHNT
                     GAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYNVRSIVLD
                     GVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQFHELLSK
                     LHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNEIETALSA
                     PIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSDSPDFCEL
                     WPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTINGDGHTL
                     ALTGVNLCV"
BASE COUNT          427 a          306 c          286 g          359 t
ORIGIN      
        1 tttcaaagtt gttttaacgt tacttctacc agtaaccgta tctgcccagc agatacagtg
       61 gcaatcctgt atggccagtc aattcaacca ctggtttggt gaggaaaaac cgtctcctga
      121 cttactatgt ggttatttgt ctgttccatt aaaatataca gacacaggcg gagatgcttc
      181 ttatgaaaaa aaatcacaag tcaaactagc gttgacaaaa ttgccggcaa aaagcaagca
      241 taaaggaagt atcctgataa taagtggtgg tcccgggtta ccaggcataa atccttatat
      301 taactttgac tggccagtca caaatcttcg tgagtcatgg gatattattg gatttgatcc
      361 tcgaggcgtc ggacagtcca ctccgacaat aaactgccgg caatcagata cagagactca
      421 ggaaaacata accgaaaagc aacaagtatt aaataaaatt aatgcctgta tccataatac
      481 cggagccgaa gtcattcgcc atatcggctc taacgaggct gtatacgata ttgatcgtat
      541 taggcaagcc ttgggggata aacaactgac agccgtggcg tattcgtatg gaactcaaat
      601 tgcagcctta tatgcagaac gttttcccta caacgtaaga tctatcgttc ttgatggagt
      661 cgtcgatatc gatgacctgg aggacaactt cacatggcaa ctcaaacagg cacagagtta
      721 tcaggaaacg tttgatcgct ttgcatcctg gtgtgcgcgt acaaaaagtt gcccgctttc
      781 ttcagacaga gataaggcaa taactcagtt ccatgagcta ttatcaaaat tacatcacaa
      841 acctttatta gacagtaaga gagaaaatat atcttcagat gaactcatat cattaacaac
      901 agaccttctg ctatggcgtt catcatggcc aacccttgca actgccatac gccagttctc
      961 tcaggggatt gtcagtaatg aaattgaaac tgcgctcagt gctccgatag cctcagaaga
     1021 gtcaagcgat gcttcggggg taatcctctg tgtagatcag ggggatgagc aattaacacc
     1081 agaagagcga aaatcccgaa aagacgctct tgcgaatgcc ttcccggcta ttaactttga
     1141 caatggacgt tccgattcac ctgatttttg tgaattatgg ccaatacata gcgacctgaa
     1201 caaaactcgc ctgaaaaata ctgttctgcc ctctggttta ctgtttatag cacacaaata
     1261 cgacccaaca acgccctgga ttaatgcccg taagatggca gagaaatttt ccagcccgtt
     1321 actaacaata aatggtgatg ggcatacatt agctctcacc ggagttaatt tatgtgta
//
LOCUS       DAZFRJ010000446         1374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000446 DAZFRJ010000000
VERSION     DAZFRJ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.449"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..507
                     /locus_tag="ACHKUN_005089"
     CDS             <1..507
                     /locus_tag="ACHKUN_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663154.1"
                     /translation="AITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRN
                     NSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNG
                     VEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKK
                     STAGISIS"
     gene            576..1199
                     /locus_tag="ACHKUN_005090"
     CDS             576..1199
                     /locus_tag="ACHKUN_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6663155.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSASGDWRTDGFIVGVGYKF"
     gene            1264..>1374
                     /locus_tag="ACHKUN_005091"
     CDS             1264..>1374
                     /locus_tag="ACHKUN_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6663156.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNT"
BASE COUNT          334 a          318 c          405 g          317 t
ORIGIN      
        1 gcaattaccg cgaataccgg cacgctcaat aatgtcacca ttaacgagaa ctgtgtcatc
       61 agagggaaac tgtctgcaaa ccagattgaa ggcgacctgg tgaagacggt ggggaaagcc
      121 tttccccgga ataacagtta tgccagcggg acggtaaccg tcacagttta cgatgaccag
      181 ggattcgacc ggcagattat cattcccccg gtgctgtttc gcgggacgaa acaccagaat
      241 ttcaacagcc cgaatcagca gtcgtactgg tattccacct gtaagctgca ggtgctgaag
      301 aacggggttg agattttcca tgaaccggca acggatgtca gccgggtgtt ctcatcggtg
      361 atagatatgc cggcagggcg gggtcatgtc accctgacgt ttaatgtgtc gtcggccggt
      421 gcgaacaact ggacgccgac aacgtacatc agtgatttac tggttgtggt catgaaaaaa
      481 tccacggcag ggatcagtat cagctgacgg tttattaacc cggacgggca cctcaggagg
      541 tgcctttttt attgactgaa aacaaagagg taatcatgcg gcatttatac gcaacgatat
      601 tattgtttac taccctgctg gcaggaattg cctttcctgc acaggctgaa agcggacacg
      661 gtgcattttc cgtgggatat gctcaggttc acccgggcgg cgtaccggca ttgtccggta
      721 ccggtgcgcg tgcaggtgat ttaaaaggga ttaatgtgaa ataccgttat gagttcacgg
      781 atcacctggg cggcattgtc gcgctgagtt atgcatcggt gaagaaaagt gacacgatga
      841 agacgggtga aaataccttc cattatgaaa gcctgcgcgg tcgttatgtc agtctgatgg
      901 ccggccctgt ctggcagctc agtgagcggg tcagtctcta tggcatggcc gggatggcgt
      961 acacccgctg gtctgacagt gttcaggatt accggcgtga tgaagtgaaa ccggggtatg
     1021 tgaaggagac caccaccgcc agtgatggcc atactgcgcg tcatctgtcg ccggcctgga
     1081 atgccgggat tcagttcagt cccgtagaga cggtggttat tgaccttgct tatgaaggtt
     1141 ccgccagtgg cgactggcgc actgacggtt tcatcgtggg tgtcggttat aagttctgat
     1201 tagccaggta acacagtgtt atgacagccc gccggttcag gcgggctttt ttgtggggtg
     1261 aatatggcag taaagatttc aggtgtactg aaagacggca caggaaaacc ggtacagaac
     1321 tgcacaatcc agctgaaagc aaaacgtaac agtaccacgg tggtggtgaa cacg
//
LOCUS       DAZFRJ010000447         1367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000447 DAZFRJ010000000
VERSION     DAZFRJ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.450"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            145..306
                     /locus_tag="ACHKUN_005092"
                     /pseudo
     CDS             145..306
                     /locus_tag="ACHKUN_005092"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            290..355
                     /locus_tag="ACHKUN_005093"
     CDS             290..355
                     /locus_tag="ACHKUN_005093"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO6663157.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            471..728
                     /locus_tag="ACHKUN_005094"
     CDS             471..728
                     /locus_tag="ACHKUN_005094"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6663158.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(775..1344)
                     /gene="nleF"
                     /locus_tag="ACHKUN_005095"
     CDS             complement(775..1344)
                     /gene="nleF"
                     /locus_tag="ACHKUN_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO6663159.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          393 a          323 c          284 g          367 t
ORIGIN      
        1 tgagtcaact atttagatga tattagtgac ctgtttcaga gcattagcgc aaggcgattc
       61 tttgtcttct tgcactaatt ttttatcata aaaatgttcc tagcactggg catcaatatc
      121 gcaggtcaga aagagctcct ggggatgcgg ctggccgaaa atgaaggggc gaatttctgg
      181 ttcaatgtgc tgactgaact gaaaaaccgc ggtctgaacg atatcctcat cgcctgtgtg
      241 tatggcctga aagaattccc ggaggcccgc atccagttat gcatcgtgca tatggtgcgc
      301 aacagcatgc gcttcgtgtc atggaaggaa tacaaagccg tcactcgcga cctgaaagcg
      361 attagcctcc cacagaagag gcaggccagc aggcactgga agcgtttgct gcggcctggg
      421 actgccgcta tccgcagata agccggtgct agctgtcaaa ctggactaac ttggcgacgt
      481 ttttcgctta tccggcagat atccgcaaag tgatctacac aacgaacgcc atcgagtcgc
      541 tgaacagtgt gatccggcat gccatcagga aacgcaaggt gttcccgacg gacgagtcag
      601 taaaaaaagt ggtgtggctg gcaatccagg ccgcgtcaca gaaatggaca atgccgttaa
      661 gggactggcg aatggcaatg agccgcttta ttatcgagtt tggtgaccgt cttgacggtc
      721 acttttaaga aaaggcattt acacagaatc ctaaacaggc tcacaggcct ctaatcatcc
      781 acattgtaaa gatcctttgt tgtaagtaag atctggtacc ctaataatat caaaaggatt
      841 aaaatcatca ctgcatcctt ccacgaggca tttcattgct cgtagatcac catatagctc
      901 tgaagagtga tgcggacata gcagattata aactttacat ttcgaatctt ctttgccttg
      961 tctactttcc tcaattttta ctttcaatga atgcagaaaa aaaagatccc tgatataact
     1021 atccctgtcc tctatcgtca ttcccgccct cagcgctccg acaacctcat cagtattttc
     1081 atttctcacc tcaaatacac tatttctctt atccatcagc ccacttacaa aagcacttag
     1141 ctcttgaaaa atttcctcaa gctcattatt atttctggca catgaatata tagagaccat
     1201 aacatcaagt ttatcttgta attcaggaga aagaaagtga ttatgattaa gctcacttac
     1261 tgattccgga gtcgaagggt tacctcttcc tgatacatac atccatgaat aaagatttgc
     1321 tgaagaacca cttgttggta acatatcaaa acccccttaa caaataa
//
LOCUS       DAZFRJ010000448         1366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000448 DAZFRJ010000000
VERSION     DAZFRJ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.451"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1366
                     /locus_tag="ACHKUN_005096"
     CDS             <1..>1366
                     /locus_tag="ACHKUN_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO6663160.1"
                     /translation="SSLRALVAKLQRMLFGRSSEKSREKIEKKIARAETRITELQNRL
                     GEAQLQLTSMAGETAPKTSDSPVRKALPATLPRDRQVISPAETECPVCSGKLKPLGES
                     ISEQLDIINTAFRVIETVRPKLACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKF
                     AEHLPLYRQSEIYARQGVELHRNTMGRWVDIMGEQLRPLYDELKHYVLMAGKVHADDT
                     PVNVLEPGQGKTRTGRLWVYVRDDRNAGSTMPAAVWFSYSPDRKGIHPQQHLADYRGI
                     LQADAYAGYNALYESGQVTEAACMAHARRKIHDVHVRHPTTVTGEALRRIGELYAIEA
                     EIRGSPAEERLAVRKARTVPLMQSLYEWLQGQMSTLSRHSDTAKAFTYLLKQWDALNE
                     YCRNGWVEIDNNLCENALRVVALGRRNYMFFGSDGGGESAAVMYSLIGSCKLNGIEPE
                     TWLRH"
BASE COUNT          338 a          379 c          403 g          246 t
ORIGIN      
        1 cagctcactc agagcgctgg tcgctaaact gcagagaatg ttgttcggtc gcagcagcga
       61 gaaaagccgc gagaagatag aaaagaagat cgcacgggca gaaacgcgta taaccgagct
      121 ccagaacagg cttggtgagg cgcagttgca actcacctca atggccggag agacagcgcc
      181 gaaaacatca gactctcccg tccgcaaagc acttccggca acacttcccc gtgacaggca
      241 ggttatctcc ccggcagaaa ccgaatgccc cgtctgcagc ggcaaactga aaccgctggg
      301 agaaagcatc tctgaacaac tggatatcat caacaccgcg ttcagggtaa tcgaaacggt
      361 tcgcccaaaa ctggcctgca gccggtgcga ctgtatagtt caggctccgc agccaccaaa
      421 acccatcgag cgcagttacg ccagtccggc tctgctggcc cgcataatca tggctaagtt
      481 cgccgagcat ctgccgctgt accgtcagtc ggaaatctat gcccgccagg gcgtggagct
      541 gcaccgcaat acgatggggc gctgggttga catcatggga gagcagcttc gcccgctgta
      601 tgatgaactg aagcactatg tgctgatggc gggtaaagtg catgccgatg acacgccggt
      661 aaatgtactg gagccgggtc agggtaaaac ccgtaccgga cggctgtggg tctatgttcg
      721 tgacgatcgc aacgccggtt cgaccatgcc ggcagcggtg tggttctcat actctcccga
      781 ccgcaaaggc atccacccac agcaacatct ggcggactac agaggtatcc tgcaggccga
      841 tgcatatgcg ggttacaatg ctctttacga aagcggtcag gtaaccgaag cggcttgtat
      901 ggcacatgcc cgacgcaaga tccacgatgt acatgtccgc catccaacga cagtaacggg
      961 agaagcgctc cgtcgtatcg gggaactgta cgctatcgag gctgagatcc gcggcagtcc
     1021 ggcagaagag cgactggcgg tcagaaaagc cagaacggta ccgctaatgc agtcgttgta
     1081 tgagtggctc caggggcaga tgagcacgct gtcgcgccac tcggatacag cgaaagcgtt
     1141 cacctatctg ctgaagcaat gggacgctct gaacgaatac tgccgcaatg gctgggtgga
     1201 gatcgacaat aacctgtgtg aaaacgccct ccgggtagtt gcactggggc ggcgtaacta
     1261 catgttcttc ggctctgatg gtggaggcga gagtgcggca gtgatgtaca gcctgatcgg
     1321 tagctgcaaa cttaacggaa tcgagccgga aacgtggcta cgccac
//
LOCUS       DAZFRJ010000449         1359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000449 DAZFRJ010000000
VERSION     DAZFRJ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.452"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..791)
                     /locus_tag="ACHKUN_005097"
     CDS             complement(<1..791)
                     /locus_tag="ACHKUN_005097"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO6663161.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATA"
     gene            complement(855..>1359)
                     /locus_tag="ACHKUN_005098"
     CDS             complement(855..>1359)
                     /locus_tag="ACHKUN_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO6663162.1"
                     /translation="HETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRI
                     HEAELLDHIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVL
                     IHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKK
                     AYEVFFI"
BASE COUNT          283 a          436 c          338 g          302 t
ORIGIN      
        1 tgtgccgtgg cggccttact ggtttccagt tcacgcagac gcttcaggtg cgcatccacc
       61 tgacggattt ccgctgcggt gttgtcgtag tgctcttcct cttccacatc cagtgtgcgg
      121 ccttcctctg cggctttgtt catgatctcc tcaagggagg ctgccagcgc cgcacgcttg
      181 ttttcaaaac ttttaatctg ttcaccagta ttcattgctg acttttcctt atgaaaagag
      241 gttattgact gtgccgaagc gccggcagaa gatgcgattt tcaccaccgg tttccggttg
      301 ccggacgcgg cagaaaacgg gcggtcgtaa gatttaatgg tccggatggt gcattccgca
      361 ttcgcgggca cggtgacggc agacacctcc atcagctccc agcgcagaaa atgcagtccg
      421 cctccgtcca gataagtgta ttcatggggc cggaagccca cagaaagccc cctgaccagc
      481 ccggtcttaa tggcagccca ggcctcatcc agccgggctg ccatctggga gggcatcccc
      541 ggctccggct tcaccagcat tgccgtgatt tccagccctt ccctgacccg acgcaccgta
      601 cactggccta cagggcggga atggtcatgc tgccagagaa acgggatcgt actgccaaac
      661 tccgccccct ccggctccag gatgtcacca tcccgatccg gagaaggcgt tgacgcaatc
      721 ccggtgatca cccgttcatc ctcactgaag gatttcaccg tcagcaggga acaggcccgt
      781 ttaagagtca catcagcctc ctgaaaataa aaaaaccgcc gcagcggttc atgatggtta
      841 cagggtgagc agggttatat gaaaaaaacc tcatacgctt tctttttcgg ttccggattc
      901 agggacatca gggacaccgc attgaagagc gccatcagcg ggtcaatttt tccccgtccg
      961 ctggcctgtt tggtgataag aatggcgtta cctttaggct ccacccgggc attaccgaca
     1021 caccaggcca tcaggggctg atcaccgtga atcagcaccc cttcagccag tttgcgctcg
     1081 gtggttttga tggccccgcc cagcttccag ccctggctta tccccaccac actctcatcg
     1141 gggatcccgg cttccgccag tgaatccaga atctgcccca cacctgacgg gtcaataccg
     1201 atatgatcca gtaactcagc ctcatgaata cgacgcacat actccgccac ttccgccgtg
     1261 tcatccccga cccgacggac aatcgtcatg tctccacagg ccacaaaatc ctgaaaccgg
     1321 gatgcctcac tcttccgtct gaccaccgcg gtttcatgc
//
LOCUS       DAZFRJ010000450         1329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000450 DAZFRJ010000000
VERSION     DAZFRJ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.453"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(482..1114)
                     /locus_tag="ACHKUN_005099"
     CDS             complement(482..1114)
                     /locus_tag="ACHKUN_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO6663163.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          359 a          273 c          270 g          427 t
ORIGIN      
        1 taatttagcg tttatttaag gcgtaatatc ttctataaat aacccgcaaa atatcgtcgt
       61 agctgcactc gatattttta aaatcctgta tcaagtgctg aaaatcattt catcgccact
      121 tccaattcct ttctgcatgg taagtagtga ggacgaaaag aagaatatga taaatattga
      181 ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc
      241 gccggtgatt tacaagtaag ggtatgatta cgctttttaa agcgtaccat aaaacataac
      301 ataataaatg atttgtgatg acacgggatt attgggattt actttgagga agataaaatg
      361 cgtgtttata gaaccgcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt
      421 cataaaaaat tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc
      481 attagttcac aaacagtgct tcgctgtcga cgatgattcc atcttccgca cctttaagcg
      541 ccaggtttgc ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat
      601 tacgagtctg tttaagcggc atcagatatt ggttttccat tacgccaaca ccggccaggc
      661 gaagttcttc cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta
      721 tcgcggcttc tttttcggga gttgctacat aactctcaga aattccgaca gatgcattaa
      781 tgacaatata ttggtcatca ttgagattgg tctttttacc tggcttagcg aactttgccc
      841 attcggtact gtcatctgac agcaaagcgg aagcttcatt ggtcagttct tttgcctttt
      901 ctggatcgcc atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac
      961 cttgttcaga gatacgctct gctgctgccg ttttctgggt ttgctgaact tgctgagttt
     1021 gctgttgtgc cgctaccggt gcattatcag cagcccatac agttgaagat gccagaattg
     1081 ctgtgaccat cgtagccata attaaacgtt tcatacttac ctcattcttt aacagtttta
     1141 gaaaacgccg ccatcatatt gggtgagcat gaaatgagaa atcagagagc aacggtcatt
     1201 ttgaaagggt tatcttacag ttgtagttat tatcgcaaga agtattttag tcttacaatt
     1261 gtaggaatct gcttcctgtc gcagagaact attcttatgt accacaaaga gatgtcattt
     1321 attatatcg
//
LOCUS       DAZFRJ010000451         1323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000451 DAZFRJ010000000
VERSION     DAZFRJ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.454"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..>1323
                     /gene="nleA"
                     /locus_tag="ACHKUN_005100"
     CDS             40..>1323
                     /gene="nleA"
                     /locus_tag="ACHKUN_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO6663164.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKGPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSN"
BASE COUNT          414 a          281 c          289 g          339 t
ORIGIN      
        1 atattattaa tggatataaa catgcaataa ggatttatca tgaacattca accgatcgta
       61 acatccggaa tcaccacaca aaacaatcga catcatcacg cagaacaaac gtcccctaca
      121 caaataccgc aatccgaatt acctaatgga tgcgaaacgg gatttgttgt tcatatccca
      181 gaggatatgc agcgacatgc accggaatgc ggtgaaacaa cagctctact gagcttgata
      241 aaagatgaag gtctgctctc tgggctggat aaatatcttg cacctcatct tgaagaaggc
      301 tctgcaggaa aaaaagcatt ggatatgttt ggtttattca atgtctctca gatggcatta
      361 gaaataccca gcaccgttcc gggtatctct ggtaaatatg gtgtccagct aaacattgta
      421 aaaccagata ttcatcctac atcaggtaat tattttttac agatattccc tctgcatgat
      481 gaaataggtt ttaattttaa agaccttcct ggcccgttaa aaaatgcatt aagcaacagt
      541 aatatatcaa ccactgcagt gtcgactatt gcatcgactg gaacatcagc cactacttcg
      601 acggtaacca ccgagccaaa aggcccaata ccatggtttg gattaacagc tcaagtggtt
      661 cgtaatcatg gtgtagaact tcctatagtc aaaactgaaa atggatggaa gcttgttgga
      721 gaaacaccac ttactcctga tgggccgaaa gcaaattaca cggaggagtg ggttatcaga
      781 ccgggagaag cagattttaa atatggtgca tctccattac aggcaactct agggctggag
      841 tttggcgcac atttcaagtg ggatttagat aaccctaata ctaaatatgc cgttcttacc
      901 aatgctgccg caaatgcgct tggtgcttta gggggatttg cagtatccag atttgctagt
      961 acagatccaa tgttaagtcc tcatatcggt gcaatggttg ggcaagcagc agggcatgcc
     1021 atacagtata atacccctgg attaaagcca gacactattt tatggtgggc tggtgcgaca
     1081 ctgggggctg ccgatttaaa caaggccgag tttgaagtag ctagattcac tgactatcct
     1141 cgtatatggt ggcacgcaag agaaggagct attttcccca ataaagcaga tattgaacat
     1201 gccacaggtg ctgatatacg cgcaatggaa gaaggtatcc ctgttggaca gcggcatcca
     1261 aatccagagg atgtggtaat cgatatcgaa agcaatggct taccacatca taatccatca
     1321 aat
//
LOCUS       DAZFRJ010000452         1311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000452 DAZFRJ010000000
VERSION     DAZFRJ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.455"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            299..592
                     /gene="iss"
                     /locus_tag="ACHKUN_005101"
     CDS             299..592
                     /gene="iss"
                     /locus_tag="ACHKUN_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO6663165.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHKUN_005102"
     CDS             complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHKUN_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO6663166.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1082..>1311)
                     /gene="rrrD"
                     /locus_tag="ACHKUN_005103"
     CDS             complement(1082..>1311)
                     /gene="rrrD"
                     /locus_tag="ACHKUN_005103"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO6663167.1"
                     /translation="EVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQ
                     LRRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          333 a          317 c          303 g          358 t
ORIGIN      
        1 tcagcacagt aaaacccggt gtgcatcgtt tttgattatt cccgcacact cacgcagaag
       61 gaattccccg tcgggctacg gtcatggtta atgcgggaat acggcgacga tacagcgcag
      121 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
      181 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      241 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      301 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      361 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      421 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      481 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      541 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      601 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      661 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      721 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      781 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      841 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      901 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      961 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
     1021 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
     1081 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1141 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1201 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1261 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgacttcg a
//
LOCUS       DAZFRJ010000453         1298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000453 DAZFRJ010000000
VERSION     DAZFRJ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.456"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..735
                     /locus_tag="ACHKUN_005104"
     CDS             1..735
                     /locus_tag="ACHKUN_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663168.1"
                     /translation="MAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKDLTPGEM
                     TAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAY
                     KIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKV
                     TPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTGVKAGKV
                     SIPVISGNGQFATVAEVTVTEAGAAG"
     gene            749..1180
                     /locus_tag="ACHKUN_005105"
     CDS             749..1180
                     /locus_tag="ACHKUN_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6663169.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            1207..>1298
                     /locus_tag="ACHKUN_005106"
     CDS             1207..>1298
                     /locus_tag="ACHKUN_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6663170.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYF"
BASE COUNT          323 a          296 c          402 g          277 t
ORIGIN      
        1 ctggcgccgg taaaaggtgc cggtaccact ctgtgggttt acaccggcaa gggtgatgct
       61 tatgcaaacc cgttgtcaga tgatgactgg cagcgactgg ctaaggtgaa ggatctgacg
      121 ccgggcgaga tgacggcaga atcctacgat gataactacc tggatgatga agacgcggac
      181 tggagcgcga ccgggcaggg acagaaatct gcaggtgata ccagttttac actggcctgg
      241 aaaccgggag aagaaggtca gaaagggctt ataggctggt ttgaaagcgg ggatgtgcgg
      301 gcctataaaa tccgttttcc gaatggcacg gtggatgtgt ttcgtggctg gatcagcagt
      361 atcggtaagg cagtgacggc gaaagaagtg atcacccgaa cagtgaaagt gaccaacgtg
      421 ggcaaacctt ccgtggcgga agaacgcagc gaaattacgc cggccactgc aattaaggtg
      481 acaccgacat ccggtaccgt ggaaaaagga aaaacaacca ccctgactgt ttcttttgag
      541 ccggaaagtg caaccgacaa gacgttcaga gcggtttccg ccgatccgtc aacgggaacc
      601 attgctgtga aagatatggt gatcactgtg acgggggtta aggctggaaa agtgagtatc
      661 cccgtgattt ccggtaatgg tcagtttgcc acggtagctg aagtcaccgt tactgaagcg
      721 ggcgctgcag ggtaaacgga ggtcatacat gtttctgaaa acagaacaat ttgaatataa
      781 cggtgtgtct gtcacgcttt ccgaattgtc tgcgctgcag cgtattgagc atcttgccct
      841 cctgaaacgg cgtgcagaac aggcagaatc cagcggcaac ctgcaggtaa gcgtggaaga
      901 tctcgtcaga accggcgcgt ttctggtggc gatgtccctg tggcataacc atccgcagaa
      961 aacggggtca ccgtcaatga atgaggccgt gatgcagatt gagcaggaag tcctgaccac
     1021 ctggcctgct gatgccattg cccgggcgga agatgtggtg ttgcgtctgt ccgggatgag
     1081 cgggcctgtt catgtggata cggatattac cgaagtggcg aaaaataacg cgctgactga
     1141 tgatgatttt tctgcgggaa agtcttcgac ggcgagctga attttgccct cagactggcg
     1201 cgtgagatgg ggaggcctga ctggcgcgcc atgcttgccg ggatgacatc caccgaatat
     1261 gccgactggc gacattttta ccgcatgcat tattttca
//
LOCUS       DAZFRJ010000454         1287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000454 DAZFRJ010000000
VERSION     DAZFRJ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.457"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            137..238
                     /locus_tag="ACHKUN_005107"
                     /pseudo
     CDS             137..238
                     /locus_tag="ACHKUN_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(648..883)
                     /locus_tag="ACHKUN_005108"
                     /pseudo
     CDS             complement(648..883)
                     /locus_tag="ACHKUN_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1054..>1287)
                     /locus_tag="ACHKUN_005109"
     CDS             complement(1054..>1287)
                     /locus_tag="ACHKUN_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO6663171.1"
                     /translation="CPITLAIPQRGVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLS
                     REPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT          383 a          302 c          230 g          372 t
ORIGIN      
        1 attgattttg ttgaactctt tttttgtttt gcaggagtta ctcctcttac cttattgtcc
       61 actcgatgac caacacattc acaacccaaa ccagaatatc ctatctatga agcatgtatc
      121 cgcacgcaga ttaattatgg tttacattgt tctaccacaa tagtcgcatc ctaaatgggt
      181 gcggttgagg atcaccatcc ttgaagagga tgcccgccac tttaactgca tgacagggct
      241 atttaaacaa tcactaaaaa tcgcccaaga gcattctgtt tctataatgt attccaagag
      301 attaaaaaat aatatcatac gttattcggc agagttcgta tgtactacgg cgtcccaacc
      361 atgtcccaaa acaaaaattt ctgccctata cacgttccaa aaaattcaac agcaaagaga
      421 gtctgctgac gagttgttga ttcactgatt aatacgtaaa attgataact gctggaaatc
      481 atacaatact cgtactttcg aaagttcgct aaccaggtgc tacacgcgag gtgttgcagt
      541 tttctttacg atttatcatt caatcctaat ccatgcatga catgtggtgc ctgtggggtg
      601 atcctaccta cgtaatgtgg acacagccct aatcaaggtt ctgtttttca aaacagttcc
      661 gtgctgagac agccacaagt actgtgtcgg caccaccgat tgtaatcaca ctagatctaa
      721 ttaaacacag ccgtctgcat tatttcccga ctcataaaat attagccgta aatgcattcg
      781 acttttagtg agtggaagaa actttccgcc ctagcattat cgtaacagcg cccctttgca
      841 ctcatcctgt catgcaggta tgccgcttca gaagcgcctg ataatgcgtt gaaatcagga
      901 cggtggctac ttgctattca gttcacgata caaaatgggc aatatccctt aaggaccagc
      961 gaatagcaat gagtcacttt attatcaagt ttgatgaccg ctttgatgct aacttttaat
     1021 aagagtctct tcacacaaaa tcgtgtaccg agtctacact gaataacaat cactcctcac
     1081 cgtaaagtga ccgatactgc aatcaaactc acactgctct ctggccacaa gcattgataa
     1141 tgttattggc tctctgctta gtgggtgatg catcccatca ttaattatac gagaaagagc
     1201 catttcatca tataaactac atactctgga accttcagca tttcttaaaa aaacacctcg
     1261 ttgaggaatt gccagcgtaa tcgggca
//
LOCUS       DAZFRJ010000455         1278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000455 DAZFRJ010000000
VERSION     DAZFRJ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.458"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(80..424)
                     /locus_tag="ACHKUN_005110"
     CDS             complement(80..424)
                     /locus_tag="ACHKUN_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663172.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(453..620)
                     /locus_tag="ACHKUN_005111"
     CDS             complement(453..620)
                     /locus_tag="ACHKUN_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663173.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(693..977)
                     /locus_tag="ACHKUN_005112"
     CDS             complement(693..977)
                     /locus_tag="ACHKUN_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO6663174.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(970..1113)
                     /locus_tag="ACHKUN_005113"
     CDS             complement(970..1113)
                     /locus_tag="ACHKUN_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663175.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(1123..1272)
                     /locus_tag="ACHKUN_005114"
                     /pseudo
     CDS             complement(1123..1272)
                     /locus_tag="ACHKUN_005114"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
BASE COUNT          314 a          305 c          267 g          392 t
ORIGIN      
        1 aaaagccgca ttgatgcggc gatggtaggt ctggatatca ttgagccatg aacaggcctc
       61 atcgagtgtg aggctgtggt tagtcctttc gtaactcgct gattctcctg taagtctctg
      121 gtgctttgtt tccgtgtatc ttcatttcag acttcaacag agcaacgagg gaatcccatt
      181 cgttgaggat gcctttgaat gccggaacgc gctttgcaac cttgtcgaat gaatctctga
      241 tttctggaat cttctcaaca agtgcaacgc atcgtcggaa gtctgctgcg tcatggggag
      301 cgccgaagtg atgaccatag atattctttt tcaggccaca tgcgattgag gcaagagttg
      361 cgctactgat gccaacatcg ccagtcgatt gccatttcaa aaccttcata gacaaatctg
      421 acatttcttg tctccaataa aaaaaacctc catcaggcgg cttggtgttc tttcagttct
      481 tcaattcgaa tattggttac gtctgcatgt gctatctgcg cccatatcat ccagtggtca
      541 tagcagtcat tgatgttctc tgcttcgata actctgttga atggttctcc attccattca
      601 cctgtgactc ggaagtgcat ttatcatctc cataaaacaa aatccgccgt agcgagttca
      661 gataaaagaa atccccgcga gtgcgaggat tgttattcat tgccgatatt cacctttatc
      721 gcgaacacct ttaccggttt atcaccgaag tgcggatgtg tgattgtctt gatttcatat
      781 ccgtcatacg gaacatcaat tctgcggctg gaatcgtcgc gcttcggata tccctttgtg
      841 ataatcaggc ggtcatactc ccggaacata attcgcttat tccaatagtc attacacagg
      901 cgatactctt ccgttttctc tcctcgaatc atggcatcga agtattcacc tttgacggca
      961 agttgtaggt tagccacggt taacctcctg cggagtagcc tttacaagca ctggtgcaaa
     1021 tccatcttca ttaaggttat gaatatagac ttctgttctc ctgcgctctt caacgtttaa
     1081 tattgtttct ggatcataaa cccatacttt cattcgacta ttccatgaag aaatcgcttc
     1141 agatctagtt cgtttttctg gtccttgggc accacatttg catgacacat agcgcatttt
     1201 ccctctgata ctgaatgagt atccgatgtt aagcacagtg gaaccacaga atgggcagcg
     1261 atatagattc ataaatca
//
LOCUS       DAZFRJ010000456         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000456 DAZFRJ010000000
VERSION     DAZFRJ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.459"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..186
                     /locus_tag="ACHKUN_005115"
                     /pseudo
     CDS             1..186
                     /locus_tag="ACHKUN_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            188..457
                     /locus_tag="ACHKUN_005116"
     CDS             188..457
                     /locus_tag="ACHKUN_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6663176.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWENIGGMK"
     gene            563..>1272
                     /locus_tag="ACHKUN_005117"
     CDS             563..>1272
                     /locus_tag="ACHKUN_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFO6663177.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGIL"
BASE COUNT          360 a          226 c          350 g          336 t
ORIGIN      
        1 cagcaatggc tggttcccgg atacagatgg cgcactcatc accggactga cctttcttga
       61 ccccaaagat gccacacggg ttcagggttt tttcagcatt tgcaggtcag gtttggtgac
      121 gggccgtggc aggatgtcaa ggggctggat gaagtgggca gtgatacagg cagaacagga
      181 gaatgacatg aatatactaa aaaaacttat gcagcgtctg tgtggttgcg gaaagcatga
      241 tggccgtgaa cacgtgcagt cgcttacagc acaactgcga ctggggccgg cagacatcct
      301 ggagtcagat gagaatggca ttattccgga gcaggacagg gtaatcacac aggtggtgat
      361 actggataca gataaaaagc tgatacagtg tgtggtaaga ccgctgcaaa tcctgcgtgc
      421 tgacgggacg tgggaaaata ttggcgggat gaagtaaccc gacagcttca caaaaccgga
      481 gtccggctcc ggtttttgtt gtcatgtcat ggtgatgttt gttaagaaag taaagattga
      541 ttcattttga aggttgaaat gtatgctatc accatcttct gtaaatttgg ggtgttcatg
      601 gaattcttta accagaaacc tgacttctcc tgacagtcgt attttatcct ctgtaaggga
      661 tgcagctgct agctcggata atggggcgca agtaaaggtg ggcaacagaa catatcgtgt
      721 tgttgtcact gataataagt tttgcgttac aagagaaagt catagtggtt gctttactaa
      781 tctgttgcac aggctgggat ggcctaaggg agagattagc aggaaaattg aggtgatgct
      841 gaattcatcg ccagtgaata gggctatgga aagaggtgct gttcattcga atagacctga
      901 tttacctcct gttgattatg caccgccaga gttaccgagt gtggactata acagcttgcc
      961 tgtgcctggt aatgttattg gcaaaggggg taacgctgta gtatatgaag atgctgagga
     1021 tgcaacaaaa gtcctgaaga tgtttactac atctcaaagc aatgaagagg taacaaacga
     1081 agttcgttgc ttcaaccaat attatggtgc cgggagtgca gaaaaaatat atggcgataa
     1141 tggcgatatt attggtatta gaatggataa aataaatgga gaatcgcttt taaatatttc
     1201 gtccttgccg gcacaggctg agcatgccat ttatgatatg tttgacaggc ttgagcaaaa
     1261 aggaattctt tt
//
LOCUS       DAZFRJ010000457         1266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000457 DAZFRJ010000000
VERSION     DAZFRJ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.460"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1245
                     /gene="frc"
                     /locus_tag="ACHKUN_005118"
     CDS             <1..1245
                     /gene="frc"
                     /locus_tag="ACHKUN_005118"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO6663178.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT          333 a          279 c          319 g          335 t
ORIGIN      
        1 actccacttc aaggaattaa agttctcgat ttcaccggtg tgcaatctgg cccatcttgt
       61 actcaaatgc tggcctggtt tggcgctgac gttattaaaa ttgaacgtcc cggcgttggt
      121 gacgtaacgc gtcaccagct gcgagatatt cctgatatcg atgcgcttta cttcaccatg
      181 cttaacagta acaaacgttc tattgagtta aataccaaaa cagcggaagg caaagaggta
      241 atggaaaagc tgatccgcga agctgatatc ttagtcgaga actttcatcc aggggccatt
      301 gatcacatgg gcttcacctg ggagcatatt caagaaatca atccacgtct gatttttggt
      361 tcgatcaaag ggtttgatga gtgttcgcct tatgtgaatg taaaagccta tgaaaacgtt
      421 gctcaggcag cgggtggcgc ggcatccact acgggttttt gggatggtcc gccgctggta
      481 agcgctgcag cgttgggtga cagcaacacc ggaatgcatt tgctgatcgg tttacttgct
      541 gctttgctgc atcgcgaaaa aacggggcgt gggcaacgag tcaccatgtc aatgcaggat
      601 gccgtattga acctttgccg cgtgaaatta cgtgaccagc agcgtctcga taaattgggt
      661 tatctggaag aatacccgca gtatccgaat ggtacatttg gtgatgcagt tccccgcggt
      721 ggtaatgcag gtggtggcgg tcagcctggc tggatcctga aatgtaaagg ctgggaaacc
      781 gatcctaacg cctatattta tttcactatt caggagcaaa actgggaaaa cacctgtaaa
      841 gccatcggca aaccagaatg gattaccgat ccggcataca gtacagccca tgcacgacag
      901 ccacatattt tcgatatttt tgctgaaatc gaaaaataca ctgtcactat tgataaacat
      961 gaagcggtgg cctatttgac tcagtttgat attccttgtg caccggtttt aagtatgaaa
     1021 gaaatttcac ttgatccctc tttgcgccaa agtggcagtg ttgttgaagt ggaacaaccg
     1081 ttgcgtggaa aatatctgac cgttggttgt ccaatgaaat tctctgcctt tacgccggat
     1141 attaaagctg cgccgctatt aggtgaacat accgctgctg tattgcagga gctgggttat
     1201 agcgacgatg aaattgctgc aatgaagcaa aaccacgcca tctgatatta acgggggctt
     1261 ctggct
//
LOCUS       DAZFRJ010000458         1261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000458 DAZFRJ010000000
VERSION     DAZFRJ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.461"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..397
                     /gene="ydeI"
                     /locus_tag="ACHKUN_005119"
     CDS             5..397
                     /gene="ydeI"
                     /locus_tag="ACHKUN_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO6663179.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            652..>1261
                     /gene="dgcZ"
                     /locus_tag="ACHKUN_005120"
     CDS             652..>1261
                     /gene="dgcZ"
                     /locus_tag="ACHKUN_005120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO6663180.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT          341 a          247 c          300 g          373 t
ORIGIN      
        1 tagtatgaaa tttcaggcca ttgtattagc aagttttctt gtcatgcctt atgcgttggc
       61 agatgaccag ggcggtttaa aacaggatgc agcgccaccg ccgcctcatg caatagaaga
      121 tggctatcgc ggaaccgatg atgcaaaaaa aatgaccgtt gatttcgcaa aaaacatgca
      181 cgatggcgct tcggtttcac tacgcggtaa tttgatttct cacaaaggag aggaccgtta
      241 cgtttttcgc gataagagcg gtgaaattaa tgtcgttatt cctgcggccg tctttgatgg
      301 acgagaagtt cagccggacc agatgatcaa cattagcggc agtctggata agaaaagcgc
      361 gccggccgtc gttcgggtca ctcatttgca gaaataaaaa taaattagcc tgatggcctg
      421 aactactggg ccattagggt attgggttat gctatttgtc atccttttat ttacgataat
      481 gcagaggtga tttacagaat taaacacttt tttatccgct gattttgttt taaatcacaa
      541 tgttatctca gagatgactc ataacttcgg atttctatga aaatattgac gtaattttgc
      601 ttttctctcg ttagaatagc gcgcacaagg aactgtgaaa aaggagtggc aatgatcaag
      661 aaaacaacgg aaattgatgc catcttgtta aatctcaata aggctatcga tgcccactac
      721 cagtggctgg tgagtatgtt tcacagcgtg gtcgcgagag atgccagtaa gccagaaata
      781 acggataacc attcttatgg actgtgccag tttggtcggt ggattgatca tctggggcca
      841 ctcgataacg atgaattacc ttacgttcgg ctaatggatt ctgcccatca acatatgcat
      901 aactgtggtc gggaattaat gctggctatt gttgaaaatc actggcagga cgcgcatttc
      961 gactcctttc aggaggggtt gctttctttt actgcggcat taaccgatta caaaatttat
     1021 ttgctgacga tccgtagcaa tatggatgtt ttgacgggat tgccggggcg tcgggttctt
     1081 gatgaatcct ttgatcatca gttacgcaac actgagcctc tgaatcttta tttaatgttg
     1141 ttggatattg accgatttaa attggttaat gatacctacg ggcatttaat cggcgatgta
     1201 gtattacgca ccctggcaac ttacttagcc agttggacgc gtgattacga aacggtttat
     1261 c
//
LOCUS       DAZFRJ010000459         1240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000459 DAZFRJ010000000
VERSION     DAZFRJ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.462"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..867
                     /locus_tag="ACHKUN_005121"
     CDS             109..867
                     /locus_tag="ACHKUN_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO6663181.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(920..1027)
                     /gene="yniD"
                     /locus_tag="ACHKUN_005122"
     CDS             complement(920..1027)
                     /gene="yniD"
                     /locus_tag="ACHKUN_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO6663182.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          308 a          286 c          295 g          351 t
ORIGIN      
        1 cgcgcctttt tataattcgt tacctttcca gggacgctaa tctttgatcc ttaagaaagt
       61 ttccgggacc gcgatggtct ctttttttgt tgtacggact cttgataaat gaagcttttg
      121 aagacagttc ctgccatcgt tatgttggcg gggggcatgt ttgcgtcact gaatgcagct
      181 gccgatgatt ccgtttttac tgtcatggac gatcctgcca gcgctaaaaa acctttcgaa
      241 ggtaatctga acgcaggata tcttgcacaa tcaggcaata cgaaaagctc ctcacttacg
      301 gctgacacca ccatgacctg gtatggacaa accactgcct ggtcgctgtg ggggaacgcc
      361 agtaatactt cttctaatga tgagcgttct tcagaaaaat atgcggcggg cgggcgtagc
      421 cgttttaatt taactgatta tgactattta tttggtcagg caagctggct gacagaccgt
      481 tataacggct atcgagagcg cgatgtgttg accgcgggtt atggtcatca gtttttgaat
      541 ggcccggtac acagtttccg ctttgaattc ggtccaggtg tgcgttacga caaatatacc
      601 gacaatgcca gtgaaaccca gccgctgggc tatgcctccg gggcttatgc atggcagttg
      661 actgacaacg ctaaatttac gcagggtgtt tcggtctttg gcgcggaaga cacaacgctg
      721 aactccgaaa gcgcattaaa tgttgctatt aatgaacatt ttggactgaa ggtggcttac
      781 aacgtcacat ggaactctga acctccagag tctgcaccgg aacataccga ccgtcggaca
      841 accttatcgc tgggctacag catgtaaaat tatctgccgg accgtgttat gcggtctggc
      901 tgtcttcgtt tgtcactgct cagccccaaa tcattgctgc ccaacgtagc aacaacatag
      961 cgccacagat tgccagtagc accaccacca tcttccagtg ttttttatca aagcgtttag
     1021 tcggcatacc agtgtcctgc atatctcttg tgcgtgataa cggagtgtaa caaaagggac
     1081 gggcatcaag ggattttttg ttattacagc cgttgggtgg cgttgtacac ctggtcactg
     1141 tttctgcgtt ttatgaaaaa aataaaatta tgtatcgata tatatcgtaa atagctggtt
     1201 ataaatacgt ctttatttca tcagcactaa aactcaggct
//
LOCUS       DAZFRJ010000460         1235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000460 DAZFRJ010000000
VERSION     DAZFRJ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.463"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1235
                     /gene="rhsB"
                     /locus_tag="ACHKUN_005123"
     CDS             <1..>1235
                     /gene="rhsB"
                     /locus_tag="ACHKUN_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO6663183.1"
                     /translation="EPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYG
                     WDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDG
                     GSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIH
                     LYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEI"
BASE COUNT          346 a          286 c          350 g          253 t
ORIGIN      
        1 agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg
       61 ggtatggcga cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccggaagt
      121 gacctggtac ggctgggacg gcgaccggct gaccacgata cagaacgaca gaacccgcat
      181 ccagacgatt tatcagccgg ggagcttcac gccactcatc agagttgaaa ccgccaccgg
      241 tgagctggcg aaaacgcagc gccgcagcct ggcggatacc cttcagcagt ccggcggcga
      301 agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag
      361 tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct
      421 gactgtggag cagatgcaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca
      481 cctgtaccac tgcgaccatc gcggcctgcc gctggccctt atcagtaagg aaggggcaac
      541 agaatggtgc gcagaatacg atgagtgggg caacctgctg aatgaagaga acccgcatca
      601 gctgcagcag cttatccgcc tgccggggca gcagtatgat gaggagtccg gcctgtatta
      661 caaccgccac cgctattatg acccgctgca ggggcgatat atcactcagg atccgattgg
      721 actgaagggg ggatggaacc tgtatggata tcaattgaat ccgatatcag acatcgaccc
      781 cctgggttta tctatgtggg aggatgcaaa atcgggggca tgtactaatg gtctttgcgg
      841 cacactatcc gctatgatag gtccagataa atttgattct atagatagca ccgcatatga
      901 cgccttaaat aaaataaata gccaatctat ttgcgaagat aaagagttcg ctggtttaat
      961 atgtaaggat aatagtggca gatatttctc aacagcacct aaccgaggag aaagaaaagg
     1021 atcatatcca ttcaatagcc cttgccctaa tggtactgag aaagtatcag cttatcatac
     1081 tcatggtgca gatagtcatg gagaatattg ggacgaaata ttttcaggta aagatgagaa
     1141 aatagttaaa agtaaagata acaatatcaa gtcattttat ttaggtacgc ccagtggtaa
     1201 ttttaaagca atagataacc acgggaagga aataa
//
LOCUS       DAZFRJ010000461         1225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000461 DAZFRJ010000000
VERSION     DAZFRJ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.464"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..605
                     /locus_tag="ACHKUN_005124"
                     /pseudo
     CDS             1..605
                     /locus_tag="ACHKUN_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(616..687)
                     /locus_tag="ACHKUN_005125"
                     /pseudo
     CDS             complement(616..687)
                     /locus_tag="ACHKUN_005125"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            750..896
                     /locus_tag="ACHKUN_005126"
                     /pseudo
     CDS             750..896
                     /locus_tag="ACHKUN_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            923..1027
                     /locus_tag="ACHKUN_005127"
                     /pseudo
     CDS             923..1027
                     /locus_tag="ACHKUN_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          353 a          270 c          291 g          311 t
ORIGIN      
        1 tgctctcctt gccgtaaaac atggcaacta cagttcactt acaccgcctc tcagcccggt
       61 aagcaccaga aaatcattga tatggccatg aatggcgtcg gatgtcgcgc cagtgcacgc
      121 attatgggcg ttggcctcaa cacggtttta cgtcacttaa aaaaatcagg ccgcagtcgg
      181 taacctcgcg catacaaccg ggcagtgatg tgattgtctg cgctgaaatg gacgaacagt
      241 ggggctacgt cggtgctaaa tcacgtcagc gctggctgtt ttacgcgtat gacaggatac
      301 ggaggacggt tgtggcgcac gtcttcggtg aacgcactct ggccacactg gagcgtcttc
      361 tgagcctgct gtcggccttt gaggtcgtgg tatagatgac ggatggctgg ccgctgtatg
      421 aatcacgcct gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgagc
      481 gacataatct gaatctgaga caacatctgg caaggctggg acggaagtta ctgtcgttct
      541 caaaatcggt ggagttgcat gacaaggtca tcgggcatta tctgaacata aaacactatc
      601 agtaagttgg agtcattacc tcctcccgaa ctcaaagcta tcatacagat aattactggt
      661 taaccctata tagggcattt taaagtgttg tgatcttacc cagcaatagt ggacacgcgg
      721 ctaagtgagt aaactctcag tcagaggtga ttcacaagac aaaacagttt caaccagtaa
      781 aaaaacgtaa acaccattcg catgaatttc gtagttaaga tctgaagctt gcagaatgca
      841 tcggtgttac tgccggagtt cgtgaactta gtctgcatga atcaccacag ctttacatct
      901 gtcgcagtaa acagcaaaat cagcaaactt acttaattat atcgagtgcg attacaatcg
      961 ctggcgatgg tacagttggt gtggcggtct cagtccggaa caatttgaaa atcagaatct
     1021 cacttaaaac ggcatccatt ttacaaaaga tggccgtctg tgtatttgta atgaatacct
     1081 tctacatgtt tagagtgcat aatccacgac aaggcgatat cctaaaaatt agctagcagg
     1141 caactttgat ggcatagata atgtttttag taaaaaagag ttgactgaat ataatacagc
     1201 ctcttcctac ataagaagga atgtg
//
LOCUS       DAZFRJ010000462         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000462 DAZFRJ010000000
VERSION     DAZFRJ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.465"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1222)
                     /locus_tag="ACHKUN_005128"
     CDS             complement(<1..>1222)
                     /locus_tag="ACHKUN_005128"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO6663184.1"
                     /translation="HSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGDPYNWAEGG
                     LDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFILFGKEGEK
                     FLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVWYQIFPDRF
                     CNCRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFCPIFTSASN
                     HKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSLIWLDVVRNG
                     ANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEECREYLLSI
                     VRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEGTPWLRGDQ
                     FDSLMNYPLTYGIID"
BASE COUNT          375 a          289 c          208 g          350 t
ORIGIN      
        1 atcaataatc ccatatgtta agggataatt cattaatgaa tcaaactgat caccacgcaa
       61 ccagggggtg ccctcatgcc atatctcacc caaaatataa cattcaggtt tgatatcttt
      121 aatcaccttt ctgaaatctc gccagaaatg atgatccact tcattcgcaa catccagacg
      181 ccaaccatca atatcaaaat tttgcgtcca atagcgaact atactaagta agtattcacg
      241 gcactcttca ttctcagtat ttagcttggg catttcaatt acattgccaa aggtttcata
      301 gttaaagttt ttaaaatccc attcactttt tggagtatcg ggataaacag gaaatttatg
      361 aatccagaac caatcggcgt aacgtgaatt ggcaccattg cgtacaacgt caagccatat
      421 tagagagtca gcgcctaaat gattgaatac tgcatccaac atgattttta ttccacgctt
      481 atgcgcttct tcaatcaaag tatggaaagc aatattccca cctaaatgtg gatcaacact
      541 aaaatgatcg atggtgtcat atttatggtt ggaagctgag gtaaaaatag gacaaaaata
      601 aatcccatta atacccagat cttccagata atcgagttta tcaataaccc cccacagatc
      661 cccacccata aagttgaatg aagtcggtgc actgccccac ggttctaccc cttctgggga
      721 tatttcaggt ctgcagttac agaaacggtc tggaaaaatt tggtaccata ccgtattttt
      781 tacccaggag ggggtattta gaacgtcaat tttattaaga taagggaagc aaaaaaagtt
      841 atttaagcgg cttaactctc gttcttcgca gtccggtgaa ctgatatcaa cacacctttt
      901 ctcaccaaaa agaaacttct ctccctcttt cccaaaaagt ataaaaccgt aacggcaacg
      961 gcgtttttga ggtttgaaca ctgcaaacca gtgatcatga aattcggtga cagcttcttg
     1021 ttccatctcg atctcgttgc cgccaatcca gccgaacgct tctgtcccgg ccatgttacc
     1081 gccgtcaaga ccaccttcag cccaattata tggatcgcct atccacagaa taactttatc
     1141 aacttctcct cttaatgtgc gtaaccgtac atgcaaagtt tcgttatcat aggcatagct
     1201 atcttcactt tttgctgagt gt
//
LOCUS       DAZFRJ010000463         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000463 DAZFRJ010000000
VERSION     DAZFRJ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.466"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            244..1188
                     /gene="leuO"
                     /locus_tag="ACHKUN_005129"
     CDS             244..1188
                     /gene="leuO"
                     /locus_tag="ACHKUN_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO6663185.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          328 a          249 c          282 g          363 t
ORIGIN      
        1 attcgcggta gtcatgatta gattgttttc gcaacaaaaa cattatggat tattatgctg
       61 tggtaaatga ctcattccac ggcaatggat tctgttttta tcagaacccg tatctttatg
      121 ttttccgaat tttactcatt ttgctttttc ttattttata tgcatgataa atcatattct
      181 tcaggattat ttctctgcat tccaataagg gaaagggagt taagtgtgac agtggagtta
      241 agtatgccag aggtacaaac agatcattca gagacggcgg agttaagcaa gccacagcta
      301 cgcatggtcg atctcaactt attaaccgtt ttcgatgccg tgatgcagga gcaaaacatt
      361 acccgtgccg ctcatgttct gggtatgtcg caacctgcgg tcagtaacgc tgttgcacgc
      421 ctgaaggtga tgtttaatga cgagcttttt gttcgttatg gccgtggtat tcaaccgact
      481 gctcgcgcat ttcaactttt tggctcagtt cgtcaggcat tgcaactagt acaaaatgaa
      541 ttgcctggtt caggttttga acccgcgagc agtgaacgtg tatttcatct ttgtgtttgc
      601 agcccgttag acagcattct gacctcgcag atttataatc acattgagca gattgcgcca
      661 aatatacatg ttatgttcaa gtcttcatta aatcagaaca ctgaacatca gctgcgttat
      721 caggaaacgg agtttgtgat tagttatgaa gacttccatc gtcctgaatt taccagcgtg
      781 ccattattta aagatgaaat ggtgctggta gccagcaaaa atcatccaac aattaagggc
      841 ccgttactga aacatgatgt ttataacgaa caacatgcgg cggtttcgct cgatcgtttc
      901 gcgtcattta gtcaaccttg gtatgacacg gtagataagc aagccagtat cgcgtatcag
      961 ggcatggcaa tgatgagcgt acttagcgtg gtgtcgcaaa cgcatttggt cgctattgcg
     1021 ccgcgttggc tggctgaaga gttcgctgaa tccttagaat tacaggtatt accgctgccg
     1081 ttaaaacaaa atagcagaac ctgttatctc tcctggcatg aagctgccgg gcgcgataaa
     1141 ggccatcagt ggatggaaga gcaattagtc tcaatttgca aacgctaact gaatgcagaa
     1201 taggtcaggc atgaatgtct gg
//
LOCUS       DAZFRJ010000464         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000464 DAZFRJ010000000
VERSION     DAZFRJ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.467"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            46..147
                     /gene="ydfB"
                     /locus_tag="ACHKUN_005130"
                     /pseudo
     CDS             46..147
                     /gene="ydfB"
                     /locus_tag="ACHKUN_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            1108..>1220
                     /gene="dicB"
                     /locus_tag="ACHKUN_005131"
                     /gene_synonym="ftsT"
     CDS             1108..>1220
                     /gene="dicB"
                     /locus_tag="ACHKUN_005131"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6663186.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQER"
BASE COUNT          377 a          269 c          277 g          297 t
ORIGIN      
        1 aaaatggtgc ccgtcgctgg ctggaaagaa actcaggtga gtgatatgga tttcaacaca
       61 atcatggaaa aggcttacga agaatacttc gaaggccttg ccgaaggcga agaagctctc
      121 agcttcagtg agtttaaaca ggcactcaga ataagaatgt gctctcacaa tgacgcggag
      181 cacaaatatg agaagcaaaa tcagaccgca gaaaattttg ttctggaacc cggagaaacg
      241 cttttcaaaa ttcccgttac gtgccccatt tgcggtttta catcagaaga acttgacgac
      301 tcctgtaaca atcaggaaac aaccaagtat gtcgaagatg ataccgagtg cgcacgaaga
      361 acgattatat ccacgagtcc aaactccagg accaataaat ctcactatga gagggtgatt
      421 aatccactcc cccaaaccaa taaaaaagat gccggaggac aaaaaaccca atggaacaac
      481 gggatatctg tcaaaaaaag acgttccatt aaagacaaac aacgcagcgc caacaactgt
      541 aagcgcttta taccagtaat caattttcat gttcttaagc ggatttattg gtggttgcga
      601 cattgcttaa tgaatcctta aaactgtggt gattttaagg ataccacctc gcctgacgtg
      661 gttaaaagca ggcacacaac acgaaagcgc acggcgaagt tcgtctcact gtacggtgtc
      721 gttaaattta attcgaccgt gcgcttccgg ttgtggcaac ccgcaaaatg gcgcggcggt
      781 aagtatggcg gggttattcc ttccccgttg aggacaccgg gttgtcaggt tgaccatacg
      841 cttaagtgac aaccccgctg caacgccctc tgttatcaat tttctggtga cgtttggcgg
      901 tatcagtttt actccgtgac tgctctgccg ccctttttaa agtgaatttt gtgatgcggt
      961 gaatacggct aagcgcacgc ggaacagtta aaaccaaaaa cagtgttatg ggtggattct
     1021 ctgtatccga cgttaattgt taactggtta acgtcacctg gaggcaccag gcactgcatc
     1081 acaaaattca ttgttgagga cgcgataatg aaaacgttat taccaaacgt taatacgtct
     1141 gaaggttgtt ttgaaattgg tgtcactatc agtaaccctg tatttactga agatgccatt
     1201 aacaagagaa aacaagaacg
//
LOCUS       DAZFRJ010000465         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000465 DAZFRJ010000000
VERSION     DAZFRJ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.468"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..1165
                     /locus_tag="ACHKUN_005132"
     CDS             20..1165
                     /locus_tag="ACHKUN_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO6663187.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          398 a          256 c          229 g          324 t
ORIGIN      
        1 tgagacaggg gaatctctca tgataaacgg acttaataat aattccgcat ctttagtttt
       61 agatgctgca attagaatta attctgattt taaaaagcag tggaatgata tgtcatgcgc
      121 tgaaaaatta ctaaaagttc ttagttttgg tttatggaat ccaacgtata ctcgtagtga
      181 aaggcaaaca tttcaagagt tgttaaccgt tttagaacct gtatctcccg cgcctaatga
      241 attgggcaga atatatgcta attttgcaga tggctcatcc ttaagaattt ccgtcactaa
      301 cagcgaactt gttgaagccg agattcgcac accagataat gaaaagattc ttatgctcct
      361 ggagtcaaac gaacaaaata ggttattaca atctttaccc atcaatctcc acatgccata
      421 cattcaggtt catcgtgcct tatctaagat ggacctgact gatcataagt caatgcacaa
      481 tctacttagt tttacgtcaa aactatcagc aaccttgatt cctcataata ctcaaacaga
      541 tccgctttcc gggcctacac cattcagctc tatgtttatg gatacatttc gaggactcgg
      601 caatgcaaag ctttcactca atggtgttga tatacctgta gatgcacaaa aattgcttcg
      661 cgatgcacta ggacttaaag acacacattc atcacttgcc cggaatgtta taaataatgg
      721 tatttctcgt catcatgcaa agcaaatagc aagggaaagc agcggcagcg ataaacagaa
      781 agctgaagtt gtggagtttt tatgccaccc cgaagcggca acagccatat gctcggcttt
      841 ctatcaatct ttcaatgtgc cagccttaat gttgacacat acaaggatct ctcaagccag
      901 agaatacaat gtggaaagat cgttagatgt acctaacgcg tgcattaaca taagtatctc
      961 tcaatcacca gatggaagca ttcacgttgc cagccatacc gggattctga taatggcgcc
     1021 agaagaccgc cccaacgagt taggaatgtt gacgaacagg acctcctatg aagtgccaca
     1081 aggtgtgaaa tgtgaaatcg atgagatggt aagaacgcta caaccaaggt atggcgcatc
     1141 tgaaacatac ctgaaaaaca tttaaaaata aaaaaatgga caccacacac tttatttatc
     1201 aaaacaa
//
LOCUS       DAZFRJ010000466         1158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000466 DAZFRJ010000000
VERSION     DAZFRJ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.469"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..252
                     /locus_tag="ACHKUN_005133"
     CDS             <1..252
                     /locus_tag="ACHKUN_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6663188.1"
                     /translation="IWYQSDAFLPPESIFTLTHTGIMLNNKVLPVTIYNIVPFNKTFW
                     NLIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQNP"
     gene            323..439
                     /locus_tag="ACHKUN_005134"
                     /pseudo
     CDS             323..439
                     /locus_tag="ACHKUN_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(533..664)
                     /locus_tag="ACHKUN_005135"
     CDS             complement(533..664)
                     /locus_tag="ACHKUN_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6663189.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT          383 a          297 c          188 g          290 t
ORIGIN      
        1 atctggtatc aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca
       61 ggtatcatgc tcaataacaa agtgctacct gtaaccattt acaacatagt accattcaat
      121 aaaacattct ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta
      181 ttgaatgaat gctttaataa ccgttgcact ctgcaaatat gtccttatgg gctaaaacaa
      241 caaaatccat aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag
      301 aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc
      361 agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca
      421 cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca
      481 accgcctgaa ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt
      541 ctggcgtttt ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac
      601 cgtcagccag cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa
      661 tcacttttta ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg
      721 aagttcatcc ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa
      781 ttctgaatta gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat
      841 aataacgtct cttcccaaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa
      901 ggcctttgct cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga
      961 cgcctcatca atattcactc tacccacacc atatgaatga taaatattta atactgaaaa
     1021 aaccattaga ccgtataaca aacactcaat caatacttaa cagaactttt atttttgaca
     1081 aacatataat attttcaaca atatactgag ccaggtatat tccagtataa ggctctgccg
     1141 gaagaaatct ggaagaat
//
LOCUS       DAZFRJ010000467         1152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000467 DAZFRJ010000000
VERSION     DAZFRJ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.470"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..205
                     /locus_tag="ACHKUN_005136"
     CDS             59..205
                     /locus_tag="ACHKUN_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663190.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            433..639
                     /locus_tag="ACHKUN_005137"
     CDS             433..639
                     /locus_tag="ACHKUN_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6663191.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            704..928
                     /locus_tag="ACHKUN_005138"
     CDS             704..928
                     /locus_tag="ACHKUN_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663192.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          277 a          263 c          323 g          289 t
ORIGIN      
        1 tgctgattga ttttgtcctg gctgatacag gcaaagggaa aataacaaag gcggattgat
       61 ggggtggcta atgatatcag ataaactcat aacgctggtg aagagcctct gtgtacttgt
      121 cggcatttca tttttagtca tgctggttgc cattttcttt tccaccgcct ggcgagtcct
      181 gacgttatcg ggactggtgg ggtgaaagag agatgaaccg tgttctgtgt gtggtgatta
      241 ttgtcatggc ggttggctgt ggtgcgctgt ggctggcaac aaaccattac cgtgacaacg
      301 cgctcaccta caaagcgcag cgcgataaaa aagccagaga gctggaacag gcgaatgcca
      361 ccattactga catgcaggtg cgccagcgtg atgttgctgc gctcgatgca aaatactcga
      421 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc
      481 gtaagcgcct gcggatcaac gccacctgcc ccggtaccgt gcgtgaagcc accggcacct
      541 ccggcgtggg caatgatgcc gccgtcgaac tctctccggt tgctggacga aacgttctcg
      601 gtatcagaga cggaatcatc agcgaccagg cagcattgag aatgcttcag gaatatatcc
      661 gcactcagtg tattaactag tatttttgtt attcggagaa tgcatgaaga aattattggt
      721 aaccgtaaag ccttttcagg gaacaattcc gttccgtatt ttgcagcgtg gtcgtgttct
      781 tgttgaaggt tcgttcagtg gtaaatgtac gcaattacac tccaggacct ttcaggtgaa
      841 tgccacgaat gaagagctaa ccgttgagtg tacgatgaat gccgctaaat gccgcatggt
      901 atccgctgca ttacagccag tgtgttgagc gaccttatta ttcatgcgcg gtattgtcgc
      961 cgtattcctg cattaacaga gaccgcagcc cgaccgggag aatcctctgc gcgagtgtgc
     1021 ggggataatc aaaaacgata cacaccgggg tttaccgcgt taacggagcg cggcgttgtc
     1081 ccctcatggt cgctggtccg gtgcgatggt ggaagaagcc ggatgtttat cactattaat
     1141 tgataacaca ga
//
LOCUS       DAZFRJ010000468         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000468 DAZFRJ010000000
VERSION     DAZFRJ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.471"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1147
                     /locus_tag="ACHKUN_005139"
     CDS             <1..>1147
                     /locus_tag="ACHKUN_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO6663193.1"
                     /translation="KAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPLS
                     LLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAVL
                     AALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIRQAAENLA
                     RLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNF
                     LSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWT
                     SDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVG
                     ALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRA"
BASE COUNT          265 a          271 c          381 g          230 t
ORIGIN      
        1 ataaggctgc catgcgcaca ctgcccgcac agttcacgga tattgtcact cagcttgccg
       61 gtggtcagaa tcccttcctt atcatgctgc agcagggggg gcagatcagc gattcattcg
      121 gtggaccgct cagcctgctt accctgctga aggaggaact tctcgggatc agggatgcct
      181 ctgaatcatc agaggagtcg ctgtcagata cggcaaatgc actggctgaa aatgcccgga
      241 atgccggtga gctgggacga tttatgtcgg tggcccgtgt ggcggcaggt ggcggggttg
      301 ccgtactggc cgcgcttgct gccgccgcct ggcaggcaga gcaggctgac cgggccttat
      361 tgcgttcact gatcctgacc ggaggggcgg ctgccaccac aacggcagaa ttgtggaaaa
      421 tggccggggt gatcagcgat gaagccggtg gtggtatcag acaggcggca gaaaatctgg
      481 cccgtctggc agaaagcggg aaatataccg ccgggcagct acggatcatg ggggaaacct
      541 ctcagagatg gctgcagacg gtgggggacg atgccgggaa ggtggaaaaa gcctttgaag
      601 ggattgcagc agatccggtg aaggcgctgg cctccctgaa tcagcagtat aacttcctga
      661 gcgtttccca gttacgccat attgatgagc ttgagcgcac gaaaggtaaa caggctgcgg
      721 tgacggaggc gatgtccctg tttgcggatg tcatgaatgc acgtctggag caacttgata
      781 aagcggccac gccggtggaa aaaatctggg acgatgttaa aacctggact tctgacgcat
      841 gggcatggat aggtgatcat acactggggg cactcagtct gatcactgac gtggtggccg
      901 gaaccgttga acaagtgaag ctgctgcttg tgcaggggga tctggcgctg gctgaattta
      961 ttcagtcagc ctgggaaacg acaaagaatg tgcccggcgt tggtgcgttg tttggtgaac
     1021 tggcagaaga gaaccgcgta tttattgaga aaacaaaacg cgatgaactg gcgctgagaa
     1081 aatccattgc ggaacgggat gcgcgtatac gccaggggga aatggggtac atcaaccgct
     1141 cgcgtgc
//
LOCUS       DAZFRJ010000469         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000469 DAZFRJ010000000
VERSION     DAZFRJ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.473"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..779
                     /locus_tag="ACHKUN_005140"
     CDS             <1..779
                     /locus_tag="ACHKUN_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6663194.1"
                     /translation="TRHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          247 a          308 c          303 g          269 t
ORIGIN      
        1 tcacccgtca cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac
       61 tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc
      121 gggaagtacg gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc
      181 ccggggtggg cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc
      241 tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg
      301 atgagtattt ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga
      361 cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc
      421 ctgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg
      481 tacatccggt tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg
      541 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg
      601 agctgcgcac ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc
      661 ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg
      721 ctgttcgtga cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa
      781 cctgtcgtga ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt
      841 actgtctctc ttgtccgtgc aatagctcaa taatagaata aaacgatcaa tatctatttt
      901 atcgatcgtt tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat
      961 acgtacagaa agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga
     1021 cctccggcac tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta
     1081 ttcaccctca atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZFRJ010000470         1126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000470 DAZFRJ010000000
VERSION     DAZFRJ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.474"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..202
                     /locus_tag="ACHKUN_005141"
                     /pseudo
     CDS             1..202
                     /locus_tag="ACHKUN_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            331..576
                     /locus_tag="ACHKUN_005142"
     CDS             331..576
                     /locus_tag="ACHKUN_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663195.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            548..958
                     /locus_tag="ACHKUN_005143"
     CDS             548..958
                     /locus_tag="ACHKUN_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO6663196.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            955..>1126
                     /locus_tag="ACHKUN_005144"
     CDS             955..>1126
                     /locus_tag="ACHKUN_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6663197.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRAR"
BASE COUNT          338 a          228 c          293 g          267 t
ORIGIN      
        1 tgagtttatg ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt
       61 tattgttctg catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt
      121 gtttggcgct gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag
      241 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
      301 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      361 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      421 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      481 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      541 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      601 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      661 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      721 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      781 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      841 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      901 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      961 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
     1021 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
     1081 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgagga
//
LOCUS       DAZFRJ010000471         1121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000471 DAZFRJ010000000
VERSION     DAZFRJ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.475"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..123
                     /locus_tag="ACHKUN_005145"
     CDS             <1..123
                     /locus_tag="ACHKUN_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO6663198.1"
                     /translation="GKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQE"
     gene            137..377
                     /locus_tag="ACHKUN_005146"
                     /pseudo
     CDS             137..377
                     /locus_tag="ACHKUN_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            397..728
                     /gene="sctI"
                     /locus_tag="ACHKUN_005147"
                     /pseudo
     CDS             397..728
                     /gene="sctI"
                     /locus_tag="ACHKUN_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            725..>1121
                     /gene="sctJ"
                     /locus_tag="ACHKUN_005148"
     CDS             725..>1121
                     /gene="sctJ"
                     /locus_tag="ACHKUN_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFO6663199.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDV"
BASE COUNT          370 a          193 c          235 g          323 t
ORIGIN      
        1 ggtaaacaat atgtaagatt taatgtgaat ttgaaaaatc aggacacaaa taatagttca
       61 tttagctacg gagataaccg attcgagaag tctcaaggta gcaactggac atttcaggaa
      121 taattgagga ggacttatgg cagattggaa tggttatatt atggatatca gcaatcaatt
      181 tgatcagggc gttgatgatc tgaaccagca agttgaaaaa gcgttggagg atttagccaa
      241 ccaatccctc cgacacgaaa tttcttgccg aatatcaaag tgcattagct gagtatacat
      301 tatatcgaaa tgcgcaatct aacgttgtaa aagcgtataa agatcttgat tcagcaatca
      361 tacaaaactt ccgctaaact agataaggat tttattatgt tagtaagcaa tatgccacct
      421 attgatagag ctgagcaatc aactgctcat gaaatacagc aagcgaaagt gattgattta
      481 aatgaccggg ttctcaatct tgacaacccg gatgataaaa tgatctccgc ttttgccaac
      541 tatgctgtac aaactgaaaa ttggcagcaa aatgcgttac aagcattaag gtctgataaa
      601 gaggggttaa ctcctgaaaa attattagtc ttgcaggatc atgtgcttaa ttataatgtc
      661 gaggtttcat tggtggaaca ttagcaagaa agattgtcgc tgcagttgag acgttgaccc
      721 gctcatgaaa tatatatcat tattattgtt tatcctgctg ttgtgcggct gcaagcagca
      781 ggaattattg aaccatcttg atcaacagca agctaatgat gtccttgctg ttttacaaag
      841 acataatatt aatgctgaaa agaaggatca aggcaaaaca ggattttcaa tatttgtcga
      901 accaacggat tttgcttctg cggtggattg gctaaaaatc tacaatctac cgggtaagcc
      961 tgatattcaa atatcgcaga tgttccctgc agatgcactc gtttcatctc cacgagcgga
     1021 aaaagcaagg ctatattctg ctattgagca gcggttagag caatctctca aaattatgga
     1081 tggcattatt tcttcgagag tacatgttag ttacgatgtt g
//
LOCUS       DAZFRJ010000472         1121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000472 DAZFRJ010000000
VERSION     DAZFRJ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.476"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..637
                     /locus_tag="ACHKUN_005149"
     CDS             62..637
                     /locus_tag="ACHKUN_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO6663200.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLPHP
                     LTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            710..>1121
                     /locus_tag="ACHKUN_005150"
     CDS             710..>1121
                     /locus_tag="ACHKUN_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFO6663201.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTSNKILMEKINSCLFRPDSNHFSCPESFL"
BASE COUNT          322 a          184 c          262 g          353 t
ORIGIN      
        1 tgggggatgt ttgttacagc tatttaagtc tggagttcaa attaaaatag ggagctttgt
       61 tatgccatta acctcagata ttagatcaca ttcatttaat cttggggtgg aggttgttcg
      121 tgcccgaatt gtagccaatg ggcgcggaga tattacagtc ggtggtgaaa ctgtcagtat
      181 tgtgtatgat tctactaatg ggcgcttttc atccagtggc ggtaatggcg gattgctttc
      241 tgagttattg cttttgggat ttaatagtgg tcctcgagcc cttggtgaga gaatgctaag
      301 tatgctttcg gactcaggtg aagcacaatc gcaagagagt attcagaaca aaatatctca
      361 atgtaagttt tctgtttgtc cagagagact tcagtgcccg cttgaggcta ttcaatgtcc
      421 aattacactg gagcagcctg aaaaaggtat ttttgtgaag aattcagatg gttcagatgt
      481 atgtacttta tttgatgccg ctgcattttc tcgtttgact ggtgaaggct taccccaccc
      541 actgacccgg gaaccaataa cggcatcaat aattgtaaaa catgaagaat gcatttatga
      601 cgataccaga ggaaacttcg ttataaaggg taattgaaat gaacattacc ctttatttta
      661 tttaatgaaa catcctgcaa actgatatga attactgaat gaggttttta tgcctgttac
      721 caccttaagt atcccaagta tatctcaatt atctcctgca ggagtacagt ctttgcagga
      781 tgctgccaga cttgaaagtg gaataagaat atccattggt agtggccaat attctgttca
      841 ctatgtccag ctactggatg gattttcagt tgaaccggtg agaggaggct tactggatag
      901 gctattgggg cgtgagcatc gaatggagag aagggctgtg gctctggaaa ggcaattaaa
      961 tggaggtgtc gattttttaa gtagtgttaa taactatttt cagagtgtca tggcagaaca
     1021 cagagaaaat aaaacaagta ataaaatatt aatggaaaaa ataaattctt gtttatttag
     1081 acctgattct aatcactttt cttgcccgga gtcatttttg a
//
LOCUS       DAZFRJ010000473         1121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000473 DAZFRJ010000000
VERSION     DAZFRJ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.477"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..703)
                     /locus_tag="ACHKUN_005151"
                     /pseudo
     CDS             complement(1..703)
                     /locus_tag="ACHKUN_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          292 a          271 c          195 g          363 t
ORIGIN      
        1 tttcttctct gccgtgactc ttttcactca ttgcgtaact gtcatgctct ggattattta
       61 attctttcag cggaaatttt tcctcaaagg ctttatttag ccgcccctgg tttcctttta
      121 ccgcgaataa ataatcacct ccctgttttt gtatcttttc tgcaatatct ttctggcagc
      181 ccatcgcatc agttgtgatg atttttcctt taatatccat catgttaaga agttcaggaa
      241 tggctgtaat ctcattggat ttctcatccg tcttgatctg tccgaggacc agactatgca
      301 ttgttgagaa cgcactaatg acatgaatcg ctcccctgcg acgactcttg tcataagagt
      361 gccggagcgt ttttccatca attgcaatga cgtctttatc atctgaagaa tggcagtcac
      421 gcatccagtt aataaagcac tcgtgaaatt ttgcaggact gatacaggat acaactctgg
      481 caatgatatc gtgaacagga ataccatttt caaaatcacc atatcacttc aaaacatcgg
      541 gatgtgtttc cccaaaatcc tctatatctt cccagccttc tgcaccagaa ataacggcac
      601 aaatagtcaa cagtagaata tccgataatt tatgttccac tttccaggct tgtctgtaat
      661 cggggataat agaaatatgt tccatcaatt ttttaagttc cattttgttc tccttaatta
      721 tgtaaggagt atttgatcat gtataagcaa taaaaaacag cttcaggtaa tgaggaatat
      781 ctcaattttt aaacataaaa tgcgaattat ttagtacaaa aagtgaactt ttgatgatct
      841 ttccctaatc tcacatagga tcagatctcg tctgatcgtt ccccctgcgc tcactaccat
      901 ggctctttac cacagcagcc ggggttgttt gggaccgact tccgacagca gatcccggtg
      961 ggcctgccac catctcttca caagcttatg cttattacat gcaaaacgtc ccgttttgca
     1021 tctttgtgcc tacggcatcc ctgtgatctt tccctgaatc tgcccctgag aacctgaatc
     1081 aaacgtctga ctcgcagaac catctgtttc tcgcgctctg t
//
LOCUS       DAZFRJ010000474         1120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000474 DAZFRJ010000000
VERSION     DAZFRJ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.478"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            630..992
                     /gene="yfdP"
                     /locus_tag="ACHKUN_005152"
     CDS             630..992
                     /gene="yfdP"
                     /locus_tag="ACHKUN_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO6663202.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            1058..>1120
                     /locus_tag="ACHKUN_005153"
     CDS             1058..>1120
                     /locus_tag="ACHKUN_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663203.1"
                     /translation="MSQNLDATAINQIHALISAQG"
BASE COUNT          308 a          268 c          287 g          257 t
ORIGIN      
        1 cttgacaatg tacccttgag atacataatg tatctaaaag aaacatgccg caggcaagat
       61 taaacaaaat ttggttgtaa cacggcgtat ggcacatgcg tcgttagcgg tctggggacg
      121 ttaaagggga caatccactc cttgctcggg caaaaaaacc aggtagccgg aatgtgcaag
      181 tcaatgatga tgctgataag acgcctaacc agcgcggcga tccggtttga cgcctgggaa
      241 gagaccaggg tgcaacgatg agggcattta tggaaccgcg acaaagtgtg gtgccgtaac
      301 ttgctaagtg ctctcagcgt tgtggtaatc cgcgaaatgg cgcggcggta agtatggcgg
      361 ggttactctt tccccgttga ggacaccgga ttgtcaggtt gaccatacgc ctgagtgaca
      421 accccaccac aacagccact gctttggcgg taccagtttg tacacttgct tccggctggt
      481 accgctcttt ttacaaaaca gagaagagca tcaccggacg acgggctcat aacccaatcc
      541 agccgggcgg ctgccaccgc aggtgttctt ctctgttttg tggagaaacc aaccgacctt
      601 gcagggtcga tatgatgagg agcagcaaaa tggctagcga acgcagtact gatgtgcagg
      661 catttatcgg ggagctggac ggcggcgtat ttgaaaccaa aatcggcgca gttctcagtg
      721 aagtcgcttc cggtgtgatg aacacgaaaa ccaaaggtaa ggtctcgctc aacctggaaa
      781 tcgaaccgtt tgatgagaac cgtgtgaaaa tcaaacacaa actctcatat gttcgcccga
      841 ctaaccgcgg gaaaatttcc gaagaagaca ccaccgaaac gccgatgtat gtcaatcgcg
      901 gtggtcgact gactattctg caggaagacc agggacaatt actgactctt gccggtgaac
      961 ctgacggaaa actccgcgca gcaggtcatt aatatcgttc ttaattaact gattatttat
     1021 ctcatcactg aatatcttta tatagtgagg acttattatg tctcagaact tagacgcaac
     1081 cgcaattaat caaatccatg cccttatttc tgctcagggt
//
LOCUS       DAZFRJ010000475         1110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000475 DAZFRJ010000000
VERSION     DAZFRJ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.479"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1110
                     /gene="escV"
                     /locus_tag="ACHKUN_005154"
     CDS             <1..>1110
                     /gene="escV"
                     /locus_tag="ACHKUN_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO6663204.1"
                     /translation="ESYHDLILALFFFMAVMMMIIPLPTVVVDIIIAINISTALLLLM
                     LSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIYSFGNFVVGGNI
                     VVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGDMRAGVIDPLEA
                     KVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGMWQFDMPFSEAL
                     SLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIVQQVSVNSRPFL
                     ISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKDAMGADLSNSQN
                     ISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEE"
BASE COUNT          265 a          203 c          261 g          381 t
ORIGIN      
        1 agagtcatat cacgatctta ttctggctct cttcttcttt atggctgtaa tgatgatgat
       61 tattccattg ccaacagttg tggtggatat cattatcgcg attaatatat cgacagcgtt
      121 gcttttatta atgctctcaa tctatataaa aaatcctctt gagctaactt cttttcctac
      181 aatcctgttg attacgacgt tgatgcgcct gtcgctcagt gttagtacaa ccagacttat
      241 tctgctgcat cacgatgcgg gtgacattat ttactccttc ggtaactttg ttgttggcgg
      301 taatattgtt gttggcctgg ttatttttac cattatcacc attgtacaat ttatggttat
      361 tacaaaaggg gctgagcgtg ttgctgaagt gagtgctcgt ttttcccttg atggtatgcc
      421 aggaaaacaa atgagtattg atggtgatat gcgcgcaggc gttattgatc cgcttgaggc
      481 aaaagttctt cgctcccgag tgcaaaagga aagccagttt tatggttcaa tggacggggc
      541 gatgaagttt gtaaaagggg acgctattgc cggtatcatc attgttttag ttaacctgtt
      601 tggtggcgtg ctcattggaa tgtggcaatt tgacatgcca tttagtgaag cacttagcct
      661 gttttctgta ttgtctgtcg gtgatgcttt ggttgcccag atccctgcgc ttattatttc
      721 cgtcactgcg ggggttgttg ttactcgtgt tcccggtgaa agcgaaaaag aagaaaacct
      781 tgcaggtgat attgttcaac aggtttctgt taatagtcgc ccttttttaa tcagtgccgc
      841 actcatgatc gtaatggcga ttattcctgg ctttcctaca ttggtcttct tgtttctggc
      901 ggtttgcctg ttgggtatcg cctggaaatt acaaaagaaa agaacgtttg gaaccggaaa
      961 taataaggac gctatgggag ctgatttgtc taatagtcaa aatatctcac cgggtgccga
     1021 gccgttgatt ttaaacttaa gtagtaatat ttatagctca gatattacac agcagattga
     1081 ggttatgcgt tggaatttct ttgaggaaag
//
LOCUS       DAZFRJ010000476         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000476 DAZFRJ010000000
VERSION     DAZFRJ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.480"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1109
                     /gene="rhsB"
                     /locus_tag="ACHKUN_005155"
     CDS             <1..>1109
                     /gene="rhsB"
                     /locus_tag="ACHKUN_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO6663205.1"
                     /translation="VYDRSNTQVRSFTYDDKYRGRMVAHRHTSRPEIRYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     IRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYAQLLSF
                     TDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIAVKDTQGHETRYEYNIAG
                     DLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRY
                     DVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGL"
BASE COUNT          281 a          324 c          332 g          172 t
ORIGIN      
        1 agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgagccg accggaaatc cgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagc tacacgtatc agtatgagaa
      181 agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      481 aatacgccgg gaatatgatg aattgggccg tctgattcag gaaactgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacgct cagttgctga gcttcaccga
      661 ctgttccggt tatgtaaccc gttatgacca tgaccgcttc gggcagatga cggcggtgca
      721 ccgcgaggaa ggcctgagcc agtaccacgc atacgacagc cgtggacagt taattgccgt
      781 gaaagacacg cagggccatg aaacgcggta tgaatacaac atcgccggtg acctgaccgc
      841 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaggccgt
      901 ccgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      961 ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
     1021 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
     1081 acttatccgc agcgaggatg aggggctgg
//
LOCUS       DAZFRJ010000477         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000477 DAZFRJ010000000
VERSION     DAZFRJ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.481"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1107
                     /gene="espL4"
                     /locus_tag="ACHKUN_005156"
     CDS             <1..>1107
                     /gene="espL4"
                     /locus_tag="ACHKUN_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO6663206.1"
                     /translation="FSANINNTAQTNEHQTLSELFYKELEDKFSGKELATPLLKSFSE
                     NCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPIFEEYLMEIL
                     PQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQN
                     VDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKM
                     ESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESKED
                     IKHYSLMDFMNVDYSLLKWSNDHIINQSVAIIPALPKEQLLMLKGSVDEITPPLSPAT
                     MNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTA"
BASE COUNT          393 a          217 c          196 g          301 t
ORIGIN      
        1 ctttctctgc aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat
       61 tgttttataa agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa
      121 aaagcttctc agagaactgt agacaaaatg gtcgtcatat ctttagcaac aaggattttg
      181 tcattaaatt ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata
      241 aaaacgaaaa cacgacactt actcaaacca ttgccccaat atttgaagaa tacctaatgg
      301 aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata
      361 gaaaagaaaa agaattccca agaattaaac ttaatggtca atgttatttt ccggggcgac
      421 cccaaaaccg tatagtatgc cgacacattg ctgcacaata tattaatgat atttatcaga
      481 atgttgatta caaaccccat caagatgatt actcttcagc tgaaaaattt ctcactcact
      541 tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct gaggggcgtt
      601 gtgttgctgc ctgtggtgat ttcgggctag ttatgaaggc atatttcgac aagatggaat
      661 caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct ttgacggtcc
      721 ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg gtttatgatc
      781 ctaatgtcac taacgataaa ataagaatta tgagcgaaag caaagaggat attaaacact
      841 attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca aatgatcata
      901 ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta ttgatgttaa
      961 aaggatctgt ggatgaaata acccctccat tatcaccagc aacgatgaat ttgctaatgg
     1021 caattggtca gaatcaccaa cttacgcaac tgatgattca gctccagaaa atgccagaac
     1081 tacatagaac agaaatgttg actgcct
//
LOCUS       DAZFRJ010000478         1106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000478 DAZFRJ010000000
VERSION     DAZFRJ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.482"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1106)
                     /locus_tag="ACHKUN_005157"
     CDS             complement(<1..>1106)
                     /locus_tag="ACHKUN_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFO6663207.1"
                     /translation="ELDKLVPHLITEHKIYGRDRITPRTVSAEDGVIKKQNNPDDNMM
                     QYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYIGIYDSSLK
                     FNCKAENGEFWAQATALRFVLGCYESTRNESLLTSLTQAAQCIMAGYPKETSHPFVVE
                     QGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQYKNVIDPG
                     YLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIGDEWITGRT
                     ANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHPILHAIMYC
                     KTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAV"
BASE COUNT          327 a          231 c          206 g          342 t
ORIGIN      
        1 agacagcagc ctcctcatga gtaggggagt atttatatct ggtattaaac tgattactgt
       61 ccggatgttt cctttcattt gcttcataac aattatcagt cttacagtac attatcgcgt
      121 gaagtatggg atgctgcatg ccaaaactag cattatagaa tagccactca gcatcatcgg
      181 ccagaccaat attagtggtc agttttatta acataacata agtgctaaat agttcatgaa
      241 cagaacaata ttcatagcct gtataagtgg cattagctgt gcgtcctgta atccactcat
      301 caccaatggg agcgccagat ggtgtaagat aatatttcaa ctgggataaa aaaatatcta
      361 atgcagtttg atactgcggc cgttggctcg ctgcgatggc taatgcccta atatgctcat
      421 acgtatgtac gccatgacct ttaagaaggt aaccagggtc tatgacattt ttatattgca
      481 gatcttgctc cgacacgcat cctgcggaat aatcttcata cagccacaag caatatgaga
      541 gatattcaat gttattggtt atttcataaa ggcggtagaa aacatctgtt attgttaatc
      601 cgtggctatg tccagcaaaa ccctgttcca caacgaaagg atgtgacgtt tctttagggt
      661 agccagccat aatgcattgt gcggcttggg taagagaagt taataaactc tcattacggg
      721 tggactcata acatccaagc acaaaacgca aggcagtagc ttgtgcccag aactctccat
      781 tttctgcttt acagttaaat tttaatgatg aatcataaat tccaatatag ccatcatcat
      841 cttgcgtatc gataagttcc aaacataaat cgctcgcttt ttcctgccat tctttattat
      901 ctaacaagta ggcactgcgg caatatccat ccttccaatt cgattgagtt tcactatccc
      961 accaataata ttgcatcatg ttatcatcgg gattattctg ttttttaata acgccatctt
     1021 cagcagatac agtgcgtgga gttatacgat ctcttccata tattttatgc tccgtaataa
     1081 gatggggaac taatttatcc agttct
//
LOCUS       DAZFRJ010000479         1103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000479 DAZFRJ010000000
VERSION     DAZFRJ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.483"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..227)
                     /locus_tag="ACHKUN_005158"
     CDS             complement(<1..227)
                     /locus_tag="ACHKUN_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663208.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRET"
     gene            complement(205..923)
                     /locus_tag="ACHKUN_005159"
                     /pseudo
     CDS             complement(205..923)
                     /locus_tag="ACHKUN_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(920..>1103)
                     /locus_tag="ACHKUN_005160"
     CDS             complement(920..>1103)
                     /locus_tag="ACHKUN_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6663209.1"
                     /translation="PAVLPDGLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELA
                     LAEKLGHAVIYQEVAQ"
BASE COUNT          247 a          299 c          235 g          322 t
ORIGIN      
        1 ctggtttctc ttttcgccac agagagcatc cagtcaaatg gctccacaac tgcaccgcag
       61 attttacagc ggacctgacg ctctttttcg tcaacccgga cagaggcgtg atggcaatat
      121 ggtctttccg atggctcata gagaaaatta acctgattac gtgggtcatc ctcttttacc
      181 ggaaataaaa cgatattgct taactcatct tctggtttta tttccatgct cctctccttt
      241 gatgcgaatg ccagcggcgc ggattgcagc gatgacttca gaaactttgt atgccattac
      301 cgtttggtaa tcatcgtgaa aatctgttcg atgaagcatg ctgctacgtt ctgggagcga
      361 tatttcccgt gcttccagtt ctgcaatgcg attctctgcg gattccaacg ccgcaaccaa
      421 ttcgtctaca gttccggcag cttgcagtgc gtaatcggta atagccatct catgatcaat
      481 ttcagtaccg ttctcattcg ttgaggtgat agcaaaataa tcagagtcga tttcgttatc
      541 agctaagtgg cgtagcgtat cggcaacaag ccggccgttt tcgattagca gcttcccttc
      601 cgtaagcgca atctcctcgt tctcctggtc gcgtgatttg atgtattgct ggtttctttc
      661 ccgttcatcc agtagtgcca gcgcaacatt tggattaaag gcagcaataa attcagcgtt
      721 tgcgtaagcc tgagcatctg tttcaaccag gcagttaacg tgacattctg caattacgcc
      781 accgggttct cctttccatt tttgacaaaa aaaactcctg ttatattccc atgctgattg
      841 cccgatgtat gccctacgat gtagcatcct ttagttgctt tctctgctgc ttcacgaagc
      901 gcctcatagt taacctctct cattgagcca cctcctgata aatcaccgca tgtcccagtt
      961 tctccgccag tgccagctct gccttagcgc ccgctgaccg ctgccagcca ttcagcatgt
     1021 aaatcgcatc cacgcaacga atcattgcca tgcaaatatc catgtagtgc ggctgtgtca
     1081 gcccgtccgg aagtactgcc ggg
//
LOCUS       DAZFRJ010000480         1101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000480 DAZFRJ010000000
VERSION     DAZFRJ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.484"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(377..595)
                     /gene="ydfC"
                     /locus_tag="ACHKUN_005161"
     CDS             complement(377..595)
                     /gene="ydfC"
                     /locus_tag="ACHKUN_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO6663210.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(667..966)
                     /locus_tag="ACHKUN_005162"
     CDS             complement(667..966)
                     /locus_tag="ACHKUN_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663211.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
BASE COUNT          251 a          290 c          258 g          302 t
ORIGIN      
        1 ggagtaaaac tgataccgcc aaacgtcacc agaaaattga taacagaggg cgttgcagcg
       61 gggttgtcac ttaagcgtat ggtcaacctg acaacccggt gtcctcaact ggggaaggaa
      121 taaccccgcc atacttaccg ccgcgccatt tcgcggattg ccacaaccgg aagcgcacgg
      181 tcgacgaaaa tttaacgaca ggctatctat gaaccagcta cctcgccgtg cgctttcgcg
      241 ttatggtctg acttttcagg gaaatatcct ttcagtaaac tgtcagtgcc ggatgctcac
      301 ccgtgtccgg cgcacgcact ccacctcacc cgtggagaac tccttaatta ccaaccttag
      361 cttcgttggt tagctattaa cgcgggtatg taatcattct ggcaatgctt aatgccgctg
      421 ctttttccag attggtgata tcctgctcca gagcggacag attttcagcc tgcttagccc
      481 tggcttcatt ggcccatttc aggtcctgcg ctgccttaat tttctggtgc atccactcat
      541 aaagttcatc atcggtatag tctggcgcga tgatgacggg ttctcgtttc tgcatgtcgg
      601 ctccttgtgg ttagcgctgc ctgcttttaa ccacgtcagg cgaggtggta tcctctgagg
      661 ggtctgttac tcgagaggaa attggttatg aatacaatca agttttcttg cccagaatgt
      721 ggtggcgaag tctttgacac atcctttaaa ccgcatggct ctgacagttt cgcgggagcc
      781 atctgcaaaa attgtggtca ccttgtaact gaagatgagt cctcgcagtt tgatgacgaa
      841 atcgttgaca atatcttcgg tgcactcacc agagactttc tgaagtaaag gcgcataccg
      901 cttagttacc gctctgataa ttcttacctg tccggcaatg gcgctgatat caatataaag
      961 cgccatcgct gtttctttgc tgatccctgg atgccttcca ttctgatgtt tgacttcgcc
     1021 cactgagaaa tcctctgttt ccccttaacg ccggggtagc ggaacaaaaa acctgctgca
     1081 tagttaaacg taatccccgc c
//
LOCUS       DAZFRJ010000481         1098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000481 DAZFRJ010000000
VERSION     DAZFRJ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.485"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..219)
                     /locus_tag="ACHKUN_005163"
     CDS             complement(<1..219)
                     /locus_tag="ACHKUN_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663212.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYA"
     gene            complement(242..1081)
                     /locus_tag="ACHKUN_005164"
     CDS             complement(242..1081)
                     /locus_tag="ACHKUN_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO6663213.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
BASE COUNT          366 a          236 c          194 g          302 t
ORIGIN      
        1 agcataagaa taaaatgtag gctcatgagt aataatgata ttggcacctt caaaaacagc
       61 ctttcttaaa acatcaattg aggcaatcca gcaggtccca attttattaa taagtgtcgc
      121 ctcagcacca aaaataattt tatcaacagt tggttgcgaa acggcaacca ttgaatttaa
      181 aaaacaatta agatctgcgg ctgttttaat atgatgcatt caacatcctt caccggtttc
      241 atcagcaata aaataatttc aacatttctg gaaaacgttt tgcccatgca gactcagagt
      301 gggtatcacc tccccataga tgataatcta tagttacatt gcgttgttta cggagactca
      361 cgcataattt ttccgcgcct gaaatataga cttcaggaaa gtcttcgcgc ataccactgg
      421 tttccagcgt accgacatcg agatatacag taagatctgc aaagatattg ttttcctgaa
      481 taaaattcag cattgcgctg tcattgaacc atagtgccgg cgaaaaaacg ccaacttttg
      541 aaaaaacgtc tagacgaaga attgcggcaa acaaactgat gtacgcaccc atagaactac
      601 ctgctatcgc tgtattatta cgatctggga gagttcgaaa attgctgtca atatagttct
      661 tgagcgtatt cacaataaaa tcaacataca acgcaccttt tcctccacta taatccacat
      721 cagggtccca gccaggtaag ggaaaagatg tgtttagcgg ccatggagaa tactcatcca
      781 tacggctgaa tttaccatga gacgatgcat tatcaatccc cacaatgata acaccgtcgt
      841 attgggaatt tgggaaaaaa gcatctaccg cctcaggtat atcccataca tgtggaccaa
      901 aagatacaga tttatcaaat aaattttgcc catcgtgcat atacaataca gggtaactga
      961 catcagattc ataataggac gcaggtagta atattcttat ttcgcgacta cttgccaatt
     1021 gtggcatcgg aaaggattgc aacacatgat atcgattccc atagttctca atatcgttca
     1081 taatttaaat taacctca
//
LOCUS       DAZFRJ010000482         1081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000482 DAZFRJ010000000
VERSION     DAZFRJ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.486"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..415
                     /locus_tag="ACHKUN_005165"
     CDS             <1..415
                     /locus_tag="ACHKUN_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO6663214.1"
                     /translation="LNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRK
                     CMIWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLN
                     NSEDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            437..>1081
                     /locus_tag="ACHKUN_005166"
     CDS             437..>1081
                     /locus_tag="ACHKUN_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO6663215.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILF"
BASE COUNT          340 a          174 c          221 g          346 t
ORIGIN      
        1 tttgaatgat aaacgaaaag ttaaactcgc ctatttattt atgttaactc aagcgggctc
       61 tccttgtatc tactacggta gcgaaatagg aattgatggt tttaaatcaa tgacgcttga
      121 gaataataga aaatgtatga tatgggatga aaacaaacaa gatcttgaat tacggcaatt
      181 tatacgttgg ttaatacgtc tacgtaaaaa acatccacaa tggtgtgtgg catcaataca
      241 atggaaagat gttgaacatc ctacggtgat cgcttatcaa cgcgataaca tcactttctt
      301 cttaaataat agtgaagata ctgccaactt tatttatgac ggaagaagta tggagatctc
      361 cggtttcagt tatgaaattg aaggtttacc tgctgctgat ctttatgatt tttaaatttc
      421 aattaggatg tttgttatga taattgatgc acacactcat attggtgact ttgtgaaaat
      481 ccgtatgcca gaagatgttt ttctggcatc tctggacaag tataatattg attttgcatt
      541 atgttcttgt ggatctgcag tagaagttga ccatgaccag aacccgattc cggatgaaga
      601 tcaagttaca cagcatgata ataatgaacg tatgctcaga cttgttcgtc aacattcaaa
      661 gcgaattggc gcgtttatgt ggattaagcc tcgtctggaa agttgtgatc aagattttga
      721 agatatgatt gccagcaatc gagatatcat ttatggcatc aaagttcacc catatcattc
      781 aaaaatggct ttcaattcag ataaagttca ggaatatata cgccttgctc aaaaatatga
      841 tctcccggta gttagccata cttctaatga ttatgaatcc agcccacagt tggtttatga
      901 gatggcactt aaatatccaa atgtgaattt tgttatgtgt catatggggc tcgcgacaga
      961 taatcaggaa gcgatcgaat tgattgcaaa acttcctaat ctctatggcg atactgcgtg
     1021 ggttagggcg gatatggttt accagtcgat aaaaatctgt ggtagtgata agattttatt
     1081 t
//
LOCUS       DAZFRJ010000483         1074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000483 DAZFRJ010000000
VERSION     DAZFRJ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.487"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHKUN_005167"
     CDS             <1..375
                     /locus_tag="ACHKUN_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO6663216.1"
                     /translation="GATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            535..672
                     /locus_tag="ACHKUN_005168"
     CDS             535..672
                     /locus_tag="ACHKUN_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663217.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            674..1012
                     /locus_tag="ACHKUN_005169"
                     /pseudo
     CDS             674..1012
                     /locus_tag="ACHKUN_005169"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            984..>1074
                     /locus_tag="ACHKUN_005170"
     CDS             984..>1074
                     /locus_tag="ACHKUN_005170"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6663218.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSSEN"
BASE COUNT          279 a          244 c          308 g          243 t
ORIGIN      
        1 ggagccaccc gggtggatgg taagcctgtt attcctggca tgaagctgtc gaaggggaaa
       61 tgcgaccagg ttaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc
      121 aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt cattctgtcc ttacaacatt
      181 ggcccaggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg agatcgaaaa
      241 ggtgcctgcg aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc
      301 tcaaataact gttatggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
      361 ggaattgaca gataagcaga atattttgct gaaaaatgcg gtttgctcac acggacggat
      421 aacacaaaat cctgcgaact gacaaaaact aagtgaataa aagtaaaaac cccatttgtt
      481 ggctgcaagc ggggttttgt gtttcctgac tccggaaaag tcaaaggaga aagtgtgttt
      541 gattttagca aactgattcg ggagattcga gtgatggctg aaaaattatc aacctggaag
      601 tacatcctta tctggctggt gtttgtgatt atggcctccg gttatttcat cggtcagata
      661 cgctggtggt gaaatgaacc gcgtactgtg cgtggtcatc attgccctgc tggtggcctg
      721 tggtgcgctt agtctggggc tgaatcatta ccgcgataac gccatcacct acaaagcgca
      781 gcgcgataaa aaagccagag agctggagct ggcaaacgca actattactg atatgcaggt
      841 gcgccagcgc gatgttgctg cgctcgatgc aaaatactcg agggaattag ccgatgcgag
      901 agctgaaaat gaaactctgc gcgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa
      961 cgccacctgc cccggtaccg tgcgtgaagc caccggcacc tccggcgtgg ataatgcaac
     1021 cggcccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag aacg
//
LOCUS       DAZFRJ010000484         1068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000484 DAZFRJ010000000
VERSION     DAZFRJ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.488"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..930
                     /locus_tag="ACHKUN_005171"
     CDS             61..930
                     /locus_tag="ACHKUN_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO6663219.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT          324 a          233 c          243 g          268 t
ORIGIN      
        1 ttatctggct tataaattaa aattaattat ttaattgaga aatggttagg gagaacctac
       61 atggccataa gcatcaaggg tgttaatacc ggtgttattc gcaagagtaa taatttcatt
      121 gcactggcgc tgaaaattaa agaacctcgc aataaagaat cgttgttctt tatgtctgtc
      181 atggaactgc gcgacctgct gattgcgctg gaaagtcgta tgcatcaaaa gcataaactg
      241 gatgctgctg cacgccttca gtatgagcaa gctcgcgata aagtcattaa gaaaatggca
      301 gaaaacatcc cagaaattct ggttgatgag cttaaaaatg ccgatattaa tcgtcgggtt
      361 aacacgctgg aacttaccga taatcaaggc gaaaatctta ccttcgtatt aacgctacat
      421 gacggtagca aatgtgagtt agtcgtcaat gagttgcaga ttgaaatgct ggcgcgagca
      481 atcattcatg caattaataa tgcggaaatg cgtgaactgg cattacgtat tacctcactg
      541 ttagatttcc tgccgttata tgacgttgac tgccaggaaa atggcaatct ggaatatgac
      601 acctattccc aaccggaatg gaaacataac ctgttcgacc attatctggc ggtgctttac
      661 cgctttaaag atgaaagtgg caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa
      721 gcaacgccgg gtaaagaaat agaggcgatc actcgccgaa tgcttgattt cagccaacga
      781 ctgaaaaaac tcgcgggtgt gccatgtcag gtctatgttc gcaccgtagc ggcgaataac
      841 gcccaaccgc taacccagga tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc
      901 accagcaaaa ctgcaccaca ggcgaaataa tctcatcttt gctaatgcga ggctggagcc
      961 atccagcctg aaatgactga taacgtgatc gttggtcatg ccggatgcgg cgtaaacgcc
     1021 ttatccggcc tacaaattct caagcattca atcagttgca atgcctgc
//
LOCUS       DAZFRJ010000485         1067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000485 DAZFRJ010000000
VERSION     DAZFRJ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.489"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(159..698)
                     /locus_tag="ACHKUN_005172"
                     /pseudo
     CDS             complement(159..698)
                     /locus_tag="ACHKUN_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          302 a          255 c          218 g          292 t
ORIGIN      
        1 gctgtttaaa ttcgggagga taattagggc agccttttcg cctgccggga gtcacatttt
       61 tctgcatatc tgatactttg gttcccacta cttatttggt ggacaccact ttgtctaatt
      121 cgtcagattc tgaccagacg gttcaggctg tacgcttaca ctgaacgcca tttaccacaa
      181 agctgattct taattgccgc agtcgtggca tcattaaaaa tgaatttata cctcacaggt
      241 tcttccgcac tgtttttctg aagtacttcc agttgtctga gggtttctga ctcatccgcg
      301 cctttgcgga gttctttatt cagttttgac aacaatgctt ttttctgagc ctttaaaagc
      361 tcttccttgt ttttccagac agccagttca cttttgtatc tggaatataa ctgcatctcc
      421 tgctggtaca ggggcttcat taataatttg tccactgcgg tttttcgttc tccggactcg
      481 gcaagcgtca ggaaaaaaag agagaccggc ccccgcagtg tattcagtct gcagacatca
      541 atcagattct ggcagacaag tgaaacagcg cccagggcag acgctgccac aagcccaagg
      601 ggagcctgcg taatttgctg agcttcaata acggcatcac gaatgagtgg aggcagcgca
      661 ctcaggggaa actcatcaca aggctgaccg aatgacatag taacctcact acacacagat
      721 ataaaagcac tgcaattctg gctattaata gcaaaaatag cagtcaaatc aatatgaggt
      781 aaaaattttt gttgatttaa tggttaaata ataaccacag tgtgttttgc tgctgaagaa
      841 agacaaaatg aatctgtgtg tcccctcctt cccttcagag cacatccact attcaaattt
      901 tacacaacca cgtacgaaca aaaagcatca tcccgggtat tgcagatacc ggtttcctgc
      961 cgggaagaat cataaacaga acaccgggaa taacgccttc aggaatcacc ccccctcctg
     1021 aagaagaata aaataaagaa gactacagtt cctgcactga atgctga
//
LOCUS       DAZFRJ010000486         1066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000486 DAZFRJ010000000
VERSION     DAZFRJ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.490"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(83..430)
                     /gene="tnpB"
                     /locus_tag="ACHKUN_005173"
     CDS             complement(83..430)
                     /gene="tnpB"
                     /locus_tag="ACHKUN_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6663220.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(427..>1066)
                     /gene="tnpA"
                     /locus_tag="ACHKUN_005174"
     CDS             complement(427..>1066)
                     /gene="tnpA"
                     /locus_tag="ACHKUN_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO6663221.1"
                     /translation="RSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFIASGI
                     AWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKVRLAKQALQP
                     GAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNP
                     FWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKGGTR"
BASE COUNT          228 a          282 c          283 g          273 t
ORIGIN      
        1 agcctgctgt gcgatcagca gggccttcag ttgctcgatg tcgtcgggga gtgtgttgtt
       61 cattcccctg ttttatcacg ggttatatcc ggatgccagg ccgttctgtc cgtttgggat
      121 gttgccacgc gatcccctcc agtagcatgg ataactgagc tggcgtcagg tgcactttcc
      181 cttcccgggt taccggccag acgaagcggc cccgttccag gcgtttggcg aacaggcata
      241 acccgtcacg atcggcccac agtattttca ccattttgcc actgcggccc cggaagacga
      301 agatatgccc ggagaacggg tcatctttca gcgtgttctg caccttcgaa gccaggccgt
      361 tgaagccaca acgcatatct gtgatgccag cgatgatcca gattctggta ccggttggca
      421 gcgttatcat cgggtacctc ctttcatttc gcggattagc gcccgtaaca tttccggagt
      481 gagagggtca aacagtttta ccacacctga tttaagatgc agctcgcacc gtgggacgtt
      541 tccgggatca ctctcagggc actcatcagg cttgttacgc cagaagggat ttgtaactgg
      601 tctggtcggc tctggcgtat cagtcagagc caccgggaca ggcatgcatt cctgtatgtc
      661 atcatcgctc agtaagccgt cctcgtactg gcttttccat ttaaacagca ggttatcatt
      721 gataccgtgc tctctggcga tccgggcaac aacagcaccg ggctgtaatg cctgcttagc
      781 cagacggacc ttaaattcac ggctgtagct ggctcgccgt tcttttcgcc atgtgccttc
      841 gctgatttga ggctctgtta attccttctt tctgttggca taaaggatgg catcaagctg
      901 agctaatgaa actgaatcgg gcaatggcca tgcgataccg gatgcaataa atcgctgaaa
      961 aagcgtatgt attgtggaat gactgagacc tagacgctga gcgatggccc ggatggtcag
     1021 tttatcttca aatcttaaac gcagagcatc aggcaaataa gaacgg
//
LOCUS       DAZFRJ010000487         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000487 DAZFRJ010000000
VERSION     DAZFRJ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.491"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            294..890
                     /gene="fimE"
                     /locus_tag="ACHKUN_005175"
     CDS             294..890
                     /gene="fimE"
                     /locus_tag="ACHKUN_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO6663222.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          314 a          191 c          214 g          340 t
ORIGIN      
        1 tcattcataa aattacacat cacttaaatt ctcctgtttc cgcacttttt tctttatttt
       61 ttaagcaact ggaagttaat ccactgcaat ctattgttat attgaatcaa atcaatgaaa
      121 atagatgtta tcacatcagt gatattttat ttttgtatga tatttaatgt aattgactga
      181 tagccacatc acttcgtgtg tggttatctt tttatctatt gggctaattt tgaccgattg
      241 aggtttccta taggtattca ttcaaatata tctcagtcag gagtactact attgtgagta
      301 aacgtcgtta tcttaccggt aaagaagttc aggccatgat gcaggcggtt tgttacgggg
      361 caacgggagc cagagattat tgtcttattc tgttggcata tcggcatggg atgcgtatta
      421 gtgaactgct tgatctgcat tatcaggatc ttgaccttaa tgaaggtcga ataaatattc
      481 gccgactgaa gaacggattt tctaccgttc acccgttacg ttttgatgag cgtgaagccg
      541 tggaacgctg gacccaggaa cgtgctaact ggaaaggcgc tgaccggacc gacgctatat
      601 ttatttctcg ccgtgggagt cggctttctc gccagcaggc ctatcgcatt attcgcgatg
      661 ccggtattga agctggaacc gtaacgcaga ctcatcctca tatgttaagg catgcttgcg
      721 gttatgaact ggcggagcgt ggtgcagata ctcgtttaat tcaggattat ctcgggcatc
      781 gaaatattcg ccatactgta cgttataccg ccagtaatgc tgctcgtttt gccggattat
      841 gggaaagaaa taatctcata aacgaaaaat taaaaagaga agaggtttga tttaactcat
      901 tgataataaa gttaaaaaac aaataaatac aagacaattg gggccaaact gtccatatca
      961 taaataagtt acgtattttt tctcaagcat aaaaatatta aaaaacgaca aaaagcatct
     1021 aactgtttga tatgtaaatt atttctcttg taaattaat
//
LOCUS       DAZFRJ010000488         1053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000488 DAZFRJ010000000
VERSION     DAZFRJ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.492"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(467..1039)
                     /gene="hdeD"
                     /locus_tag="ACHKUN_005176"
     CDS             complement(467..1039)
                     /gene="hdeD"
                     /locus_tag="ACHKUN_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO6663223.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          349 a          243 c          210 g          251 t
ORIGIN      
        1 gtctccttgt ttcgacttaa gctggcaatt ggattgccag ctttctttta acgcattgaa
       61 attacctgag taaataacgc attcatgtct tcaggttttt atatagcgtc tggagacacg
      121 gatatttatg caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta
      181 accgcccata gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa
      241 acattaaacc tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc
      301 atggagtgaa aaaaattaga tgaaattcag taggttgaaa tgatcactag caggtaatta
      361 tttcaatgat agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga
      421 tcatagtcac atcaagtgac tatgatcctg gtgacaaccg ggataattat tgctgcttaa
      481 cgaacaaact ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca
      541 gtgtggaaac cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga
      601 cgatatccag cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat
      661 accaactcat caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa
      721 tggcaaaaat gcccagctcc ggtgcgcgga tgaagaaata gccgatcaat aaataggcga
      781 ctgcgacgag gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc
      841 cgacaataag cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat
      901 cgccagagac gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa
      961 taaactggat tgctctgcga tgctttttca gcatctccag atcaaacttc aaaattgttg
     1021 ccttatctat atataacata gaaccaccct ata
//
LOCUS       DAZFRJ010000489         1052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000489 DAZFRJ010000000
VERSION     DAZFRJ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.493"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHKUN_005177"
     CDS             <1..108
                     /locus_tag="ACHKUN_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6663224.1"
                     /translation="SKESCLQARDEQKISGECLPLKKVSLYLNNETPAG"
     gene            204..>1052
                     /locus_tag="ACHKUN_005178"
     CDS             204..>1052
                     /locus_tag="ACHKUN_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6663225.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RVNQQHTENSTGKISLVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDN"
BASE COUNT          315 a          244 c          258 g          235 t
ORIGIN      
        1 agtaaagaat cctgcctcca ggcaagagac gagcaaaaaa tttctggtga atgcctcccg
       61 ctaaaaaaag tatcgctgta cctgaataac gaaacaccgg ctggataatc ctccagccat
      121 attaacacca taccaacgga ttaaaaatgc cagcaatggc agggattcgt tcaccctgaa
      181 atctgtaatg aggttaaaac aaaatgagta aggtctttat ttgcgctgct attcctgatg
      241 aacaggccat aaaagaagat agcgctgttg cggtggccac tgccattgaa gctggtgatg
      301 agcgtcgcgc acgcgcaaaa tttcattggc aatttctgga gcaattccct gcagctcagg
      361 actgcgctta taaatttatt gtctgtgagg ataaacccgg cataccccgc cctgccctcg
      421 attcctggga tgctgaatat atgcaggaaa accgctggga tgaggcgtct gcttcctttg
      481 tcccggttga gactgaatca gatccgatga acgtcacttt tgacaagctg gcccctgaag
      541 tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt gatatggtta
      601 ttagcgcaca ggaattgttg caggaagaca tggcaacatt cgacggacat atcgttgaag
      661 cgttgatgaa aatgccagaa gttaacgcca tgtatccgga gcttaagctg catgccatcg
      721 ggtgggttaa gcataaatgt aagcctggtg ccaaatggcc cgaaattcag gcagagatgc
      781 gcatctggaa aaaacgtcgc gaaggtgaac gcaaggaaac cggaaaatac acgtctgttg
      841 ttgatctcgc ccgcgccaga gtcaatcaac agcacactga aaattcaaca ggaaaaatca
      901 gcctggtcat tgctgccatt catcgcgaat acaagcagac atggaaaaca ctggatgacg
      961 aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac ggcagcatcc
     1021 atcgctgggc aaaaaatgaa gttatcgaca ac
//
LOCUS       DAZFRJ010000490         1039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000490 DAZFRJ010000000
VERSION     DAZFRJ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.494"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..636
                     /locus_tag="ACHKUN_005179"
     CDS             <1..636
                     /locus_tag="ACHKUN_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO6663226.1"
                     /translation="LQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIHVP
                     RDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGL
                     WRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRG
                     VFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDAGG"
     gene            639..965
                     /locus_tag="ACHKUN_005180"
     CDS             639..965
                     /locus_tag="ACHKUN_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6663227.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            975..>1039
                     /locus_tag="ACHKUN_005181"
     CDS             975..>1039
                     /locus_tag="ACHKUN_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663228.1"
                     /translation="MAISAGRLTQMISVLNPVLTR"
BASE COUNT          228 a          250 c          348 g          213 t
ORIGIN      
        1 ctgcagaagg cggtgaatga tgcggtcgca aatattccgc agccggcaga cggtaaaagt
       61 ctcaccccgg atgatgtgcg tccgatgctt gaacagatgg tgaaggaggc ggtaagccat
      121 attcatgttc cgcgtgatgg tcgtgactac gatccggatg ttctgcagaa ggcggttctg
      181 gatgcggtga gtgccctgcc ggctccgcag gacgggcgtg atgccacggc tctggaaata
      241 ctccccgcca ttgacgatca aaaatccttt ccccggggca cgtatgccac acaccagggc
      301 ggactctggc gggcgtatga aaaaacgcac gggatgcggg gatgggaatg cctggttgac
      361 ggggtggcgg atattgacgt cagcatgacg ggtgagcggt tgttctctgt ggtggtccgg
      421 cagagcagtg gccagcgtac ggaaaaaaca ttttccctgc cggtgatgct ctaccgcggt
      481 gtgttcagag ccggtgaaac ctaccacccc ggcgatacgg tgacgtgggg gggctcgctg
      541 tggcactgca acagtatgac cgaagataaa cccggagaag ctcattcatc agcctggacc
      601 ctggctgcaa aacgtgggcg ggatgcagga ggctgaaaat gacggcatta ctgacactgg
      661 aagagatcaa ggcacatctg cgtgtcgacc atgacgcgga tgatgacatg ctgatggaca
      721 aggttcgtca ggctaccgcc gtgctgctgg cctacattca gggcagccgg gataaagtga
      781 tccgtgagga cggtgaactg atcccgggcg aggcattaac ccggatgaag ggggctgcca
      841 tgcgactgac cgggatgctg taccggaatc cggatcttgc ggagcgggaa gaactgcttc
      901 agggggagct gccgttttct gtttccgtgc tgatttacga tttgcgttgt ccgacggtgt
      961 tatgaggagg gggaatggca atatctgcag gtcgtctgac acagatgata agtgttctga
     1021 acccggtgtt aacccgtaa
//
LOCUS       DAZFRJ010000491         1035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000491 DAZFRJ010000000
VERSION     DAZFRJ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.495"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1011)
                     /locus_tag="ACHKUN_005182"
     CDS             complement(<1..1011)
                     /locus_tag="ACHKUN_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6663229.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDS"
BASE COUNT          211 a          261 c          240 g          323 t
ORIGIN      
        1 tgaatctgat ttatgcccga gaagacgttg agcaaactta tcgccaatct gattccggta
       61 tagcctcgcc gacaggctgc gcagttcatg gaatgttggc gggtctccat caaatgagag
      121 tccagatgca tttctcgcct ttgtaaaata cttagatact gttttcgggg aaagcggttc
      181 gtgatgcgtt gatgcaatta ttgtttcact gccgctggcc tccctgcatt tctgtagtgt
      241 atcagccaat gagatattga gcgcgtcaat cgttagcgtc agcggaatgg cgagcttagc
      301 ccctgtttta ccctgctcaa tgtgaagatg gttgtcgttt atgtctgacc atttcattct
      361 gcataaatcg cctactctct gccctgtaac gacagccaaa tccattgcca gcctcagcca
      421 gattgggagc ggttcggctg catggtaaat cgcgacatat tcattagctg tcagtcttga
      481 acgccttact tctgattttg ctgtgcgggt tgctgttacc ggattcgtag ccacatgccc
      541 ctcggctatt gcctcacgaa aaacgtcaac aagggttgac ctgattaatt ttgcggaagc
      601 cgctttacct tctgctacgt aggtgtttag cattgctgcc acctctttcg ttgatatgtc
      661 agcgagcggt ttgtccggca attttcttcg gattgccctg attttgctgg cgtagtcgag
      721 tagagttttc ggcctgatac ccctctcgct gaggattgtt tcatatcggt caagccacgc
      781 atgaagagtg attgcgtcag cgcctttaat tctgtctatc agtgactcac gcctgttccc
      841 ggatagcaac tcaatattgg cctgtattgc ttcagtgatt gctatcctcc tgtctcggcc
      901 taatccgaac tctttacccg tccttgggtc cctgtagcag taatatccat tgtttcttat
      961 ataaaggtta gggggtaaat cccggcgctc atgacttcgc cttcttccca tttctgatcc
     1021 tcttcaaaag gctac
//
LOCUS       DAZFRJ010000492         1031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000492 DAZFRJ010000000
VERSION     DAZFRJ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.496"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..937)
                     /locus_tag="ACHKUN_005183"
     CDS             complement(<1..937)
                     /locus_tag="ACHKUN_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6663230.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEVARHAEEAKKNAGEAETSARNAGISASQAEEGAANADTSAGEASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNAARDATTA
                     TEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGD
                     TGPAGATGERGPRGDTGPAGPQGPKGDRGERGETGL"
BASE COUNT          155 a          364 c          248 g          264 t
ORIGIN      
        1 tcagaccggt ctctccccgc tctcccctgt cgcctttcgg cccctgcggg cctgccggac
       61 cagtatctcc tctcggcccc ctttcaccgg ttgcccctgc cgggccggtg tcgccacgct
      121 ctcctttatc tcccttcggc ccctgaggac ccgcgggacc cggttccccc tttggcccgg
      181 gaggccccac cacggtgggg attcggttta cggcctcttc cgccgctatc ctgctttgtt
      241 ccgctgactg tgcgctttct gctgactccc gggctttttc tgttgcggtc gttgcatccc
      301 tggctgcatt accggctgca ctttctgccg tctttcttga caattcagct tctgctgcac
      361 tttgtgatga ctcactggct ttttgagcgg ccgcagaggc cgaggacgag gacgcttcct
      421 ctgactgctt tgcagcggct gcactttctg ccgcctgccg ggctgactcc gatgcctccc
      481 ctgctgaagt gtcagcattt gcagcgccct cttctgcctg actggctgat atgccggcat
      541 tcctcgctga cgtctccgcc tctccggcat tcttcttcgc ctcctctgcg tgacgcgcca
      601 cctcttccac catcagttca aaacggcgca gtgcctccgg ccggacgtca tcctccgaca
      661 tggcaccgag aaaatcattc agcgtccccg gttgagaatc ttcatacacg gtgatggtcc
      721 cggcatgtga cggcgggaag ccctccacca acagaatgac gctgtactga ccgtactcaa
      781 cgtccatgct gtaacgaccg gcttcatccg gattttcaga ggccaccgta ttcaccacca
      841 ccgtgctgct ggtccgtctg gctttcagtt gaatggtgca gttctctacc ggttttcctg
      901 tgccgtcttt cagtacacct gaaatcttta ctgccatatt caccccacaa aaaagcccgc
      961 ctgaaccggc gggctgtcat aacactgtgt tacctggcta atcagaactt ataaccgaca
     1021 cccacgatga a
//
LOCUS       DAZFRJ010000493         1030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000493 DAZFRJ010000000
VERSION     DAZFRJ010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.497"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..569
                     /locus_tag="ACHKUN_005184"
     CDS             <1..569
                     /locus_tag="ACHKUN_005184"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6663231.1"
                     /translation="YFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYL
                     KLQALFARVAEEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALG
                     RRATGVELETERFEQTVREVQDLVSQNG"
     gene            615..685
                     /locus_tag="ACHKUN_005185"
     tRNA            615..685
                     /locus_tag="ACHKUN_005185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:643..645,aa:Met,seq:cat)
BASE COUNT          259 a          237 c          299 g          235 t
ORIGIN      
        1 cttactttcg tgatgcgcgt gctgccctgg ggataacggc aaaacagata gtggatgcca
       61 caggaaagaa aaacatggtg tcgcactggt ttagtgccag tcagtggcag ctaccgaacg
      121 aaagcgatta tctgaaatta caggcgctgt ttgcccgggt ggcagaagag aagcatcagc
      181 gcggtgaact ggaaaagccc caccaccagc tgctggagac gtatacttca ctgaaccggc
      241 agtatgcgga actgcagagt gaatataagc atctgcggcg gtattttggc gtgacggcgc
      301 aggtgccgta cacggatgtg tggacgcata aaccggtgca gtactatccc gggaaacatc
      361 cgtgcgaaaa accggcagaa atgctgcagc agataatcag tgcgagcagt cgtccgggtg
      421 acctggttgc agatttcttc atggggtcgg gttcgacagt caaagccgcg atggcgctgg
      481 ggcgtcgtgc aactggcgtt gagctggaga ctgaacgttt tgagcagacg gtcagggaag
      541 tacaggattt agtcagtcag aacggatgat attgcaggat tagttacgta ccgttattat
      601 cctgcgccag gccctttagc tcagtggtga gagcgagcga cgcataatcg ccaggtcgct
      661 ggttcgaatc cagtacaacg cgccacacca cacttatctg ccctgactct cttttgcggg
      721 ctttttatta caggaaagac accggacagt gaaatgttaa atgcctcaca attcaggtag
      781 ttgactgttg cctgacatgc tgagcgtttg ttaaaaaaat cctgcatgat gaatccccct
      841 gggcggcggg gcataatgac agatgtttgg ttgcgtattg tataggcaag ttgcggattc
      901 tgtctggtca ttgcagaatt caccgggagg cacccggcat catgctgtat acagagatta
      961 ggcatatatc caggctcctc atcgcaggag cctttttaca tgcaaaaaac gcccgagtag
     1021 gttcgggcaa
//
LOCUS       DAZFRJ010000494         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000494 DAZFRJ010000000
VERSION     DAZFRJ010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.498"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1026
                     /locus_tag="ACHKUN_005186"
     CDS             <1..>1026
                     /locus_tag="ACHKUN_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6663232.1"
                     /translation="NQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPF
                     YFLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTT
                     AQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRD
                     SVNQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPD
                     CGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETAD
                     HSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT
                     IDLETAGG"
BASE COUNT          344 a          248 c          261 g          173 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg ggcaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggatggc gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggaaa aagcactagc cttgggagct acggaaatca caaaagagga
      241 atacttcgca cgtaaaaacg gcacgtactc aggttcacaa caaaatactg gtgcatctga
      301 cacgaccgca caaccagagc cggtaaaagt taccgctgac gaagtaaaca aaattatgca
      361 ggcagccaat atcagccagc ctgacgccga taagttgctt gctgcctctc gcggagaatt
      421 tgttgcaggg attagcgacc cgaatgatcc gaaatgggta aaggggattg aaacccgcga
      481 ttctgtaaac cagaaccagc aagaatcgga acagaacgac cagaaagcgg aacaaaacag
      541 cccaaatacg caacaaaacg agccagaaac gaaacaacct gagccagtag cgcaacagga
      601 accggaaaaa gtctgcaccg cctgcggtca gaccggcggc ggcaactgcc ctgattgtgg
      661 cgcggtgatg ggcgacgcaa cataccagga aacattcgat gaagagtatc aggttgaagt
      721 tcaggaagat gatccggagg aaatggaagg cgctgaacat ccacacaagg agaacactga
      781 cggcaatcag catcacgata gcgataatga aactggcgag acggcagatc actcaattaa
      841 ggtgaacggt catcaagaaa tcacatccac cagcaggacg tgtgaccatc taatgatcga
      901 ccttgaaacc atgggaaaaa atcctgatgc cccgatcatc tcaataggtg caatattttt
      961 cgatccgcaa accggagata tggggccgga atttagtaag actatcgatc tggaaactgc
     1021 tggcgg
//
LOCUS       DAZFRJ010000495         1013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000495 DAZFRJ010000000
VERSION     DAZFRJ010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.499"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1013
                     /locus_tag="ACHKUN_005187"
     CDS             <1..>1013
                     /locus_tag="ACHKUN_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6663233.1"
                     /translation="IPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGS
                     GEDSRLVTTAITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPAT
                     IELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGP
                     HIKPGKDFWFYVRSVNLVGKSAFVEASGQASNDAEGYLDFFRGEIGKTHLAQGLWELI
                     DNSQLADEMAEMKTSITETRNEITQTVNKTLEDQSATIQQIQRVQKDTNDDLAALYML
                     KVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQL
                     FMN"
BASE COUNT          260 a          256 c          312 g          185 t
ORIGIN      
        1 attccaccgg cagtgcagca cctcacggtg gaggtgagcg cggctgacgg ccagtatctg
       61 gcacaggcga aatgggacac gccgcgggtg gtgaaggggg tgcgcttcag tctgcgactg
      121 accagcggaa gcggagaaga cagccgtctg gtgaccaccg ccatcactgc ggatacagag
      181 catcgttcca gtggtctgcc gctcggggaa tacaccctga cagtcagggc aattaacagt
      241 tatggccagc agggcgaacc ggccaccacc accttccgga ttaacgcgcc agcaaaaccc
      301 gccaccattg aactgacgcc ggggtatttt cagataacgg cggtcccgcg tcttgcggtg
      361 tatgacccga cggtacagtt tgagttctgg ttttcggaga caaaaatcgc agacacatct
      421 caggtggaaa cctctgcccg ttatctgggg accggcagtc agtggagtgt atccggcccg
      481 cacattaagc ccgggaagga tttctggttt tacgtgcgca gcgtcaatct ggtgggcaag
      541 tctgcgtttg tggaagccag cgggcaggcc agcaatgatg cagaagggta tctggacttt
      601 ttcagaggag aaataggaaa aacgcatctg gctcaggggt tgtgggaact gatagataac
      661 agccagcttg cagatgagat ggcggagatg aagaccagca tcacggaaac ccgcaatgaa
      721 atcacacaga cggtcaacaa aacgctggag gaccagagcg ccaccataca gcagatacag
      781 cgcgtgcaga aggacacaaa tgatgacctg gctgcgctgt acatgctgaa ggttcaaaaa
      841 acgaaagacg gcattcccta tgtggccggg attggtgcag ggattgagga tactgatggc
      901 cagccactga gcaacatact gctgctggct gaccgtatcg cgatgataaa tccggagaac
      961 ggcaacagca cgccgttatt tgtggcgcag gggaatcagc tgttcatgaa cga
//
LOCUS       DAZFRJ010000496         1000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000496 DAZFRJ010000000
VERSION     DAZFRJ010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.500"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..764
                     /gene="evgA"
                     /locus_tag="ACHKUN_005188"
     CDS             150..764
                     /gene="evgA"
                     /locus_tag="ACHKUN_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO6663234.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            769..>1000
                     /locus_tag="ACHKUN_005189"
     CDS             769..>1000
                     /locus_tag="ACHKUN_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFO6663235.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQ"
BASE COUNT          312 a          190 c          208 g          290 t
ORIGIN      
        1 atttatgccg actatttata tggtatactt gtcgaattat cttaaaggaa gctcagattt
       61 tcttattttt attgagaaaa tgagatgacg ccttatgtct gtattactac agggagaagg
      121 gagatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat gaccatcctc
      181 ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc ttagcggagt
      241 tgactgaagg gggaagcgcc gttcagcggg tggaaacact taagcctgat atcgtcatca
      301 ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg aggaagcgcc
      361 aatatagcgg aattattatt attgtctccg ctaaaaatga ccatttttac gggaaacatt
      421 gtgctgatgc tggcgctaat ggattcgtga gtaaaaaaga aggcatgaac aatatcattg
      481 cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc aaccggtttg
      541 ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa gaaattagtg
      601 tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa atgttcatca
      661 gcaacaaaac tgtcagcact tataaaagtc gcctgatgga aaaattagaa tgtaaatcac
      721 tgatggatct ttacacattc gcgcaacgta acaaaatcgg ctaatcacat gaagttttta
      781 ccctattttt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc agacgaagat
      841 tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc agtacgtctg
      901 agcaacaagg aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc agtacacaag
      961 tcccaaacgg ctaccttgtt gcataccgat tcgcagcaac
//
LOCUS       DAZFRJ010000497          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000497 DAZFRJ010000000
VERSION     DAZFRJ010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.501"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            156..788
                     /gene="yqeH"
                     /locus_tag="ACHKUN_005190"
     CDS             156..788
                     /gene="yqeH"
                     /locus_tag="ACHKUN_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6663236.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
BASE COUNT          335 a          167 c          183 g          307 t
ORIGIN      
        1 taacgagaaa tgcattttaa aatgtcttaa tcagaattat agcaaataca gattaatcgc
       61 aatttttccc agcaaaatta caaaatcatg gtgtattgac atcatcaatc accaatgaaa
      121 ttatgcaatt atatacggat agggaggttc ttaacatggg gctttgtagt cgttataaaa
      181 gtcttacatg caatagttgc tcaatgcatt gccaaataat gccagaagag tcaccgcgtt
      241 tgcagtactg cgcaaactcg tgtttttgta tgtggcccga agaaagttca tattttaatc
      301 gtggtgtggt agaaggtatc ttaacaaaaa accacaatgc gagattaagc ggatatattt
      361 ttgtcgattt ttcagtaagt tttttacgtc tattccttga aaaagactgg attgactatc
      421 ttgcaagtac tgacatgggg attgttttag tcagcgaccg caatatgcaa tcgttagcta
      481 attactggcg aaaacacaac tcagcaatat ctgctgtcat atataatgat gatggtcttg
      541 atgttgccaa tgaaaagatc agacaactat ttattggtcg ttatttatca tttaccagag
      601 gtaacacatt aactcagatg gaattcacca taatggggta tatggtttct ggttataatc
      661 catatcagat tgctgaagtt ctggatatgg atatccgtag catctacgcg tacaagcaac
      721 gaatcgaaaa gagaatgggt ggtaaaataa acgaattatt tattcgttca cattcggtcc
      781 aatattgata ctcaacgacc agccagaatc atactctgtt tatacgggca tttattgaaa
      841 tgtccgtatt ttataaatag gccgtcccct caagagtaaa caccatagta tttaaaacca
      901 tcacacgaaa aattcagaag cattacgaaa tatgaattta caaacaatag tggcataaat
      961 gttaaccatg ttaatttacg taaagtttta cg
//
LOCUS       DAZFRJ010000498          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000498 DAZFRJ010000000
VERSION     DAZFRJ010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.502"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(111..>992)
                     /locus_tag="ACHKUN_005191"
     CDS             complement(111..>992)
                     /locus_tag="ACHKUN_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6663237.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
BASE COUNT          243 a          240 c          249 g          260 t
ORIGIN      
        1 tatttagagg taacgggtga ttgcggtgct atcagccgga ataaaaaaga aatttattgg
       61 agcaggttgc tcttccccat gggaagagca tgtgtgtgaa tagccttctg tcaccttctc
      121 agcggattgt taacatttgc gtattcaaac ggactgatcc ccttctcccg gttcgcatca
      181 agaaaatcgg cccaccactg tagcatcagt cgacgttctt ccagatgttc tgctttatgg
      241 atatacgcag cacgtacacc attacgctcc tgatggctca tctgacgttc cacagcatcg
      301 cgggaccaca aacctgattc aatcaatgca ctgcacgcca tcgcccggaa tccatggcca
      361 cagacatcct gggttgtgtc ataccccatc accctcaggg ctttatttac agtattttca
      421 ctgattggtt tacgcgggtc atgtgctccc gtaaagatca gaccattttc accagcccat
      481 gtctgtagtt ctgtcagtat ggctacagcc tgtttgctga gtggcacaag atgtggtgtt
      541 cgcatcttgg agccccggtg tgagaatttt acgcctggca gaggttcgcg ttcggctgga
      601 atagtccaca tggcattgtc aatatcaatt tcagaccagc gggcatatct gagttcactg
      661 gaacgaataa aaaccagtaa attcagcata acggcaagcc gggtgacagg ctggccttta
      721 taaccgtcaa tacgcgacag aagttcaggc aggcgtgaaa gattcagagc ggggcgatgc
      781 tggcgtttta ccgtggtcaa tgcaccagcc atatccagag ccgggttata tctgatgatg
      841 ccagactgta cggcgtaacg cattacagca gatattcttt gctgcagacg actggcgatt
      901 tcataaattt gttttgcttc tgcagcacga acagggataa gcaggtccgg ggtattgagt
      961 gttgtgatat cccggttgcc gataaagggg aa
//
LOCUS       DAZFRJ010000499          990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000499 DAZFRJ010000000
VERSION     DAZFRJ010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.503"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..634
                     /locus_tag="ACHKUN_005192"
     CDS             <1..634
                     /locus_tag="ACHKUN_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO6663238.1"
                     /translation="HDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVY
                     ERSKKHGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTP
                     EGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCF
                     VYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            631..837
                     /locus_tag="ACHKUN_005193"
     CDS             631..837
                     /locus_tag="ACHKUN_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO6663239.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            834..>990
                     /locus_tag="ACHKUN_005194"
     CDS             834..>990
                     /locus_tag="ACHKUN_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6663240.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPN"
BASE COUNT          258 a          249 c          297 g          186 t
ORIGIN      
        1 ccacgacgat gaacagacgc tgctgcgtgt ggatgaggcc atcaataaaa cctatacccg
       61 ccggaatggt gcagaaatgt cggtatcccg tatctgctgg gatactggcg ggattgaccc
      121 gaccattgtg tatgaacgct cgaaaaaaca tgggctgttc cgggtgatcc ccattaaagg
      181 ggcatccgtc tacggaaagc cggtggccag catgccacgt aagcgaaaca aaaacggggt
      241 ttaccttacc gaaatcggta cggataccgc gaaagagcag atttataacc gcttcacact
      301 gacgccggaa ggggatgaac cgcttcccgg tgccgttcac ttcccgaata acccggatat
      361 ttttgatctt accgaagcgc agcaactgac tgctgaagag caggtcgaaa aatgggtgga
      421 tggcaggaaa aaaatactgt gggacagcaa aaagcgacgc aatgaggcgc tcgactgctt
      481 cgtttatgcg ctggcggcgc tgcgcatcag tatttcccgc tggcagctgg atctcagtgc
      541 actgctggcg agcctgcagg aagaggatgg tgcagcaacc aacaagaaaa cactggcaga
      601 atacgcccgt gccttatccg gagaggatga atgacgcgac aggaagaact tgccgctgcc
      661 cgtgcggcac tgcatgacct gatgacagga aaacgggtgg caacggtaca gaaagacgga
      721 cggcgagtgg agtttacgac cacttccgtg tctgacctga aaaaatacat tgctgagctg
      781 gaagtgcaga ccggcatgac acagcgacgc aggggaccag caggatttta tgtatgaaaa
      841 tgtccaccat tcccaccctt ctggggccgg acggcatgac atcgctgcgt gaatatgccg
      901 gttatcacgg cggtggcagc ggatttggtg ggcagttgcg ggcgtggaac ccaccgggtg
      961 aaagtgtgga tgcagccctg ctgcccaact
//
LOCUS       DAZFRJ010000500          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000500 DAZFRJ010000000
VERSION     DAZFRJ010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.504"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..360
                     /locus_tag="ACHKUN_005195"
     CDS             <1..360
                     /locus_tag="ACHKUN_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6663241.1"
                     /translation="YHLAGIDMPDFEREDDWWRNGQNLYLDNMEATGFYRISLPSAQP
                     GDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHA
                     SAFTGIYNDLAAASACM"
     gene            357..938
                     /locus_tag="ACHKUN_005196"
     CDS             357..938
                     /locus_tag="ACHKUN_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6663242.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQA"
BASE COUNT          211 a          260 c          312 g          199 t
ORIGIN      
        1 taccatctgg cgggaattga tatgccggat tttgagcgtg aggatgactg gtggcgcaac
       61 ggtcagaacc tgtacctgga caatatggag gcgactggtt tttacaggat ttccctgcct
      121 tccgcacagc ctggcgatat tctgctgtgc tgctttggtg cttcggtacc gaaccatgcc
      181 gccatttact gcggcaacgg tgagctgctt caccatctgc ctgaacaact gagtaaacgg
      241 gagaggtatt ccgaaaaatg gcaacgacga acgcattcag cctggcgtca ccgccactgg
      301 cacgcatctg ccttcacggg gatttacaac gatttggccg ccgcctcagc ctgtatgtga
      361 acacggcagc ggaagccatc cgggcgctgt cgttacaggt gcccggattc cgccgtcaga
      421 tgaacgaagg ctggtaccag atacgtattg ccggtgatga cacggcaccg gaggcggtgt
      481 acgcccgtct tcacgaacag ctgggtgagg gaacggtcat ccacattgtg ccgcgactgg
      541 ccggggccgg aaagggtgga ctgcagattg tgctgggggc ggcagccatc gtgggctctt
      601 tcttcacggc cggagcatcg atggcagcct ggggtgcagc gctgagtgcc ggcggttttt
      661 ctgccaccac gatgctgttt tcactgggtg ccagcatgat actgggtggt gtggcacaga
      721 tgctggcccc gaagccaaaa acaccggaat acagggcaac ggataacggt aaacagaaca
      781 cgtacttttc gtcgctggac aacatgattg cccagggtaa cccgatgccg gtgccttacg
      841 gggaaatgct ggttggctcc cgccgtatat cccaggacat cagtacccgt gatgaaggcg
      901 ggggcggaaa ggtcgtggtt atcgggcggc aggcataaaa gcgaaaaaat cccgcagtgc
      961 tcacggacag gaactgcggg ag
//
LOCUS       DAZFRJ010000501          981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000501 DAZFRJ010000000
VERSION     DAZFRJ010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.505"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>981
                     /locus_tag="ACHKUN_005197"
     CDS             <1..>981
                     /locus_tag="ACHKUN_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663243.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     MLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYL"
BASE COUNT          234 a          267 c          311 g          169 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacacctcgt gaggcgaagg acaatctcaa atccacgcag
       61 atgatgagcg tgattgacgc cattggtgaa gggccgattg aaggtccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccacctga aggctttgag
      241 tcctccgggg cggaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacat ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactggtg
      361 cagaccacgt caaagggtga ccgaaacccg acatccgtcc gactgctgat tcagttacag
      421 cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 tttctggcgt cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc
      541 cgggagacag cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg ccagcagatg acggtgaact accatatccg tggtcgcatc
      721 attcaggtac cgtcaaacta tgacccggaa aaacgcacgt acagcggtat ctgggacggc
      781 agcctgaaac cggcatacag caacaacccg gcctggtgcc tgtgggacat gctgactcac
      841 ccgcgctacg gcatgggaaa acgtctgggg gcggcagacg tggacaaatg ggcgctgtat
      901 gccattgcgc agtactgcga ccagacggtc ccggatggtt tcgggggcac agagccgcgg
      961 atgaccttta atgcgtacct g
//
LOCUS       DAZFRJ010000502          977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000502 DAZFRJ010000000
VERSION     DAZFRJ010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.506"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>977
                     /locus_tag="ACHKUN_005198"
     CDS             <1..>977
                     /locus_tag="ACHKUN_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6663244.1"
                     /translation="GSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKN
                     SKSTLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQ
                     THIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGL
                     ASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESG
                     ANLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSD
                     RWAGADFWEEQGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVTGRDR"
BASE COUNT          200 a          248 c          320 g          209 t
ORIGIN      
        1 ggtagcccga cattcgggga gtcctgtgca ccgtgggtgt ttgacctggt ggcggccctg
       61 tttggctcct acgatgcgca gaccggtgtt cgccatatca aggaagtgtt tatccttatc
      121 cccaagaaaa actcgaagtc cacgctggct gcggggatca tgatgacggc gctgttactg
      181 aactggcggc aggcggcggg ctacaccatt ctggccccga ccgtggaggt ggcggctaac
      241 gccttcaacc ctgccaggga tatggtacga cgggacgatg atctggatga cctctgtcag
      301 gtgcagaccc atatccggac catcacccat cgggtgacgg acaccaccct gaaggtggtg
      361 gcagccgatc cgaatacggt gtccggtatc aagtccgtgg ggacactgat tgatgaactg
      421 tggctgtttg gcaagcagta caaggcggaa gacatgctac gtgaagccat cggcgggctt
      481 gcctcccgtc cggaagggtt tgtggtgtat acgaccaccc agtcgaatga accgcccgcc
      541 ggggtgttca gacagaaact gcagtatgcc cgggatgtcc gtgacggcaa aattcatgat
      601 ccgcactttc tgccggtgat atttgaacac cctcctgaaa tggtggaaag cggggctaac
      661 ctgctgatgg aaaacctcgc catggtcaat ccgaatctcg gctattcagt ggatgaggcc
      721 tttctgtacc gggagtaccg taaagcccgg gaagccggtg aagagacatt ccgggggttc
      781 atgtcaaaac acgccaatgt ggaaattggt cttgccctgc gctctgaccg ctgggcgggg
      841 gctgatttct gggaagagca gggccgttgt atcagcctgg acgatatcct gcgtcgtgct
      901 gatgtggtga cggtggggat tgacggcgga gggctggatg atctgctggg gatgtatgtg
      961 actgggcgtg accggga
//
LOCUS       DAZFRJ010000503          976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000503 DAZFRJ010000000
VERSION     DAZFRJ010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.507"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..954
                     /locus_tag="ACHKUN_005199"
                     /pseudo
     CDS             1..954
                     /locus_tag="ACHKUN_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          286 a          227 c          254 g          209 t
ORIGIN      
        1 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
       61 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      121 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      181 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      241 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      301 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      361 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      421 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      481 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      541 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      601 aaagctctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      661 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtttggtctg
      721 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      781 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      841 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
      901 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
      961 aacaaatggt agtgtc
//
LOCUS       DAZFRJ010000504          971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000504 DAZFRJ010000000
VERSION     DAZFRJ010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.508"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHKUN_005200"
     CDS             <1..198
                     /locus_tag="ACHKUN_005200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663245.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>971
                     /locus_tag="ACHKUN_005201"
     CDS             234..>971
                     /locus_tag="ACHKUN_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663246.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAII"
BASE COUNT          313 a          160 c          205 g          293 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg agagatttag
      781 gtaatgtagg atataacgaa agccgctatg cattcaattt caaagttgat aataatgagg
      841 attataagtc tcttattagt gccacgcttg gatatccgac cagaataaat gtaaaagatg
      901 ttcgcggttg ggatactcga tttgatatta attttcagat ggaagaactg acagctgaac
      961 aagcaataat c
//
LOCUS       DAZFRJ010000505          969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000505 DAZFRJ010000000
VERSION     DAZFRJ010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.509"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..280)
                     /locus_tag="ACHKUN_005202"
     CDS             complement(<1..280)
                     /locus_tag="ACHKUN_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6663247.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMP"
     gene            complement(361..903)
                     /locus_tag="ACHKUN_005203"
     CDS             complement(361..903)
                     /locus_tag="ACHKUN_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6663248.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKEIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          284 a          208 c          177 g          300 t
ORIGIN      
        1 tcggcataat tttgattttt acttgctgcc cggaatttgc ccctattttt gccactggtg
       61 gcgttaacat gaaaggaacc tgaatttttt ctggtggtaa cgacgtatcg ccatcatcaa
      121 tccacgcctg cagcagcgaa gaacggtttc cctggttcat caactgcacc ataacttctt
      181 tattttctgc cgggtaaata atgcgcgtcc cgtagataac aattccggca tgtgcaggaa
      241 ggaccataga aaaaatgagt aaagataata atcctttcat cttaatgcct cttaaattaa
      301 aaagccgcca tggggatagc ggccatgttt attacctgga gaaacttaca attttattta
      361 ttaatcctgc gccacacgca taaccagaga agataacaca tcacctgatt gcactgagtt
      421 atctgtcagt ttcaggtatt ttgctttaaa cggaatagca tattgacctt ccgctgtttt
      481 ataactctca atgttaaaag gtgcagaaac atccaggaca tcgctgttat cacctaaata
      541 gagatagtaa cccacggtat cgttgctact ggctatctcc ttatcgttac cggttatttt
      601 atcgttggta atcaacgaga gttttacacc agcaacttct ttcgggcagc taatttttaa
      661 catgctgctg gttacaccga ctgcatcatc ataggtagaa tatttagctt taccttcaaa
      721 gacagtggtt ggtacagtcg gcatattaat acttactgaa tcaccacccg cttcgaattt
      781 acacggagat tctgcaatat tccccttaat agttaatgtt ccggtatcaa cgtctgcagc
      841 aaatactcca gcgctcataa aaaaagaaga aaagacagcg gcagcaataa ttgaacgttt
      901 cataatatgt tccttaataa aatttatttt tggttgttaa gagtctgtat ctcttggaaa
      961 caaatttct
//
LOCUS       DAZFRJ010000506          967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000506 DAZFRJ010000000
VERSION     DAZFRJ010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.510"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(144..815)
                     /gene="queE"
                     /locus_tag="ACHKUN_005204"
     CDS             complement(144..815)
                     /gene="queE"
                     /locus_tag="ACHKUN_005204"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO6663249.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          264 a          231 c          215 g          257 t
ORIGIN      
        1 aattaatgcc acagaatttg tagagaatgc taatgattag ccctggatgg gtaaaatgga
       61 ggagttttca gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat
      121 ttataacgct tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac
      181 aaacgccaat tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg
      241 ctaatcggct gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc cagtagttca
      301 tccagtgctt caatatcacg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc
      361 agtgcctgtg acaacacgtc atagccgccg cgcatgttca gctttggcga tacggtaacc
      421 caggtattcg gtgtgcagcg tacttcatga gttccactgg tttcgatctg gcagctaaaa
      481 ccgttctttt cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc
      541 gtaatcacca catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca
      601 ctgctcgccg ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag
      661 acttcccgat cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc
      721 gggcatccct gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc
      781 agggtctgga acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt
      841 gttaattatt gcagatcctg ccacaacaat catgtcttat taacattctg ttacaggcag
      901 atttaaagaa aaatgcgaaa catatcgtta ataattaaag ggagtaacgt attatgtcag
      961 aagaaaa
//
LOCUS       DAZFRJ010000507          965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000507 DAZFRJ010000000
VERSION     DAZFRJ010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.511"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..301
                     /gene="yddL"
                     /locus_tag="ACHKUN_005205"
                     /pseudo
     CDS             26..301
                     /gene="yddL"
                     /locus_tag="ACHKUN_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            340..>965
                     /locus_tag="ACHKUN_005206"
     CDS             340..>965
                     /locus_tag="ACHKUN_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO6663250.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTD"
BASE COUNT          343 a          186 c          162 g          274 t
ORIGIN      
        1 acgataatta aaaggattct gttaaatgaa attaaaaata gttgcggtgg ttgtaactgg
       61 tttgttagct gcgaacgtag cacacgctgc cgaagtctat aacaaggatg gtaataaact
      121 cgacctttat ggcaaggtta ccgctctacg ttattttact gatgataagc gtgacgatgg
      181 tgataaaact tatgcccgtc tcggctttaa aggagaaacg caaatcaatg atcaaatgat
      241 tggttttggt cactgggaat atgattttaa aggctataac gatgaagcca acggctcgcg
      301 cggcaagaat ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta
      361 cacaatcatc ccaggatgaa aacaatcact ttaaacgaca accatattgc acatttaaac
      421 gccaaaaaca ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc
      481 aatgacattg atcaactaat atcatcaaaa catctttggc atgtattagt taacgtcatc
      541 aacaatgatc cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac
      601 actaatgaag tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg
      661 caaaacttca cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac
      721 tacgatagac tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct
      781 caaggcagaa atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt
      841 gatatagata ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt
      901 gtgcaattag aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact
      961 gatat
//
LOCUS       DAZFRJ010000508          960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000508 DAZFRJ010000000
VERSION     DAZFRJ010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.512"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..565
                     /locus_tag="ACHKUN_005207"
     CDS             92..565
                     /locus_tag="ACHKUN_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663251.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
BASE COUNT          270 a          199 c          211 g          280 t
ORIGIN      
        1 ctgtccacaa ctaccaatag ctttacataa tgtgtgcatc taacttcaat aagttacttc
       61 attaccaata atgtctaaaa aggaaacgcg catggatatt gatacgctgg gtggccagga
      121 acttcgggat aagatttttg ctgggctcaa aatatacgag ggtaaaccct tcatcgaacg
      181 gttcggtctg tttatgggca aggcacagtt gctagagttt ggcctaaaaa aaattcttgt
      241 ttcttttccg ggatacaacc ttgatgaaga aaaattagag aggctgacat taggccagac
      301 ccgcgttgag cttaaaaagc tggggctgcg cacggactat aatgcttacc tgaagtcatt
      361 caaagatcag cgcaatacga tggctcacga gttccttgct aattttgcgg tcacacagca
      421 gcttttagac ggagctgcac ttattcgcac atttgagcgt gagttagacc atgcctgtta
      481 cgcggtggag cagttgatca tccttttcga cttcatcaac ggtgccggtg acgtaaccgc
      541 ctggcttgag cctactgccc cctgatactt cgtatgcttt tatctctagg ctggacagaa
      601 tagtaattct gtccaggtat gcagaatggc tgtctatata tcttcattag ttcacagtag
      661 gttaccggcg gctttgagcg aacagcggaa cttcgccctt taataaatga taataaattt
      721 tcattatcac tttagttgaa aatggtgtcg aagatgaaga gaaatgtcat attagcctac
      781 ggagtacttg tgaaataaat tcatagcatt ccctaacatc taaacttagg gctcactgaa
      841 cgatggtgaa ccctatgcca ttgggttaat aactattgat aattttgtga agtgcagggt
      901 taattgtcga tgaaatatca aaatcggaat ttctaatcga cccagtgctc catccctctt
//
LOCUS       DAZFRJ010000509          957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000509 DAZFRJ010000000
VERSION     DAZFRJ010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.513"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHKUN_005208"
     CDS             <1..882
                     /locus_tag="ACHKUN_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6663252.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          228 c          219 g          243 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaa
//
LOCUS       DAZFRJ010000510          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000510 DAZFRJ010000000
VERSION     DAZFRJ010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.514"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..794)
                     /locus_tag="ACHKUN_005209"
     CDS             complement(<1..794)
                     /locus_tag="ACHKUN_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO6663253.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQK"
     gene            complement(797..>955)
                     /locus_tag="ACHKUN_005210"
     CDS             complement(797..>955)
                     /locus_tag="ACHKUN_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO6663254.1"
                     /translation="TDIFITWIYKLTLNFQQYHIASVISIIIFIFLSVIAIWQFRRMK
                     SFKDDVGM"
BASE COUNT          350 a          184 c          167 g          254 t
ORIGIN      
        1 tgcttctgtg tgaatacaaa taacaatgtg ataggtactg caactaaaag cgccccagca
       61 gcaaaaagcg tataattctc tgctgaatta gaagcaatcc aactaaaaat tccaatcgcc
      121 agggtcattt tatcctcact acgaagcacc aacgttggca agatgaagtc catccatggt
      181 ccagtgaaca aaactaatcc aacgaaaacg agaatcggct tagccaatgg caatattatc
      241 tgaataaaaa tagtcagatg ccctgctcca tctattttag cggcctcatc caaagaagtt
      301 ggaatagcat caaaataacc ttttaccaac cacgtcatga agggcaatga accggttaca
      361 taaaccagaa gcaacccagt atatgtgtct atcaaattgg cctttgatag caggatataa
      421 attgcagtca ttgataaaaa tgcaggaaac atttgcaata caagaatact cattaaagta
      481 ttacgctttc cacgaaatcg ataccttgaa aaaacgtagg cggtcatcgt cacaacgaac
      541 agtgaaatta acatgttagc ggtagcaaga atgaaagtgt tcaaatacca ctgcatatat
      601 ggcgtatccg tgaacaacgt tatgtagtgg tcaaatgtaa actcgatgcc actaaatgtt
      661 gaactaaata gattattccc ttttttaaaa gatgccatta tcgtccatat cactggccca
      721 agcacaagaa tggagttgca gattaaaaaa agataaacta agagcgttga tattttacgt
      781 aaaaatttat ccattgtcac atccctacat catctttaaa tgatttcatc cgtctgaatt
      841 gccatattgc tataaccgat aagaagataa atattatgat ggagattaca gaagctatat
      901 gatattgctg gaaattcaat gtgagtttat aaatccaggt aataaaaata tcggt
//
LOCUS       DAZFRJ010000511          951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000511 DAZFRJ010000000
VERSION     DAZFRJ010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.515"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..943
                     /gene="hlyE"
                     /locus_tag="ACHKUN_005211"
     CDS             32..943
                     /gene="hlyE"
                     /locus_tag="ACHKUN_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO6663255.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT          325 a          179 c          207 g          240 t
ORIGIN      
        1 acaatttata tatttaaaga ggcgaatgat tatgactgaa atcgttgcag ataaaacggt
       61 agaagtagtt aaaaacgcaa tcgaaaccgc agatggagca ttagatcttt ataataaata
      121 tctcgatcag gtcatcccct ggcagacctt tgatgaaacc ataaaagagt taagtcgctt
      181 taaacaggag tattcacagg cagcctccgt tttagtcggc gatattaaaa ccttacttat
      241 ggatagccag gataagtatt ttgaagcaac ccaaacagtg tatgaatggt gtggtgttgc
      301 gacgcaattg ctcgcagcgt atattttgct atttgatgag tacaatgaga agaaagcatc
      361 cgcccagaaa gacattctca ttaaggtact ggatgacggc atcacgaagc tgaatgaagc
      421 gcaaaaatcc ctgctggtaa gctcacaaag tttcaacaac gcttccggga aactgctggc
      481 gttagatagc cagttaacca atgatttttc agaaaaaagc agctatttcc agtcacaggt
      541 agataaaatc aggaaggaag cgtatgccgg tgccgcagcc ggtgtcgtcg ccggtccatt
      601 tggattaatc atttcctatt ctattgctgc gggcgtagtt gaagggaaac tgattccaga
      661 attgaagaac aagttaaaat ctgtgcagaa tttctttacc accctgtcta acacggttaa
      721 acaagcgaat aaagatatcg atgccgccaa attgaaatta accaccgaaa tagccgccat
      781 cggggagata aaaacggaaa ctgaaacaac cagattctat gttgattatg atgatttaat
      841 gctttctttg ctaaaagaag cggcaaaaaa aatgattaac acctgtaatg agtatcagaa
      901 aagacacggt aaaaagacac tctttgaggt acctgaagtc tgataagcga t
//
LOCUS       DAZFRJ010000512          948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000512 DAZFRJ010000000
VERSION     DAZFRJ010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.516"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..948
                     /locus_tag="ACHKUN_005212"
                     /pseudo
     CDS             1..948
                     /locus_tag="ACHKUN_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          339 a          177 c          178 g          254 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
      301 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
      361 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
      421 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
      481 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
      541 atttaaccat caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag
      601 caggccttta gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta
      661 tgatgggtcc acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca
      721 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
      781 tgagggtgta tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
      841 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
      901 tacctggcct aacaaattgc aacctggaat atacaaaaac atggggag
//
LOCUS       DAZFRJ010000513          944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000513 DAZFRJ010000000
VERSION     DAZFRJ010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.517"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHKUN_005213"
     CDS             complement(<1..245)
                     /locus_tag="ACHKUN_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6663256.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..586)
                     /locus_tag="ACHKUN_005214"
     CDS             complement(245..586)
                     /locus_tag="ACHKUN_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663257.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(579..>944)
                     /locus_tag="ACHKUN_005215"
     CDS             complement(579..>944)
                     /locus_tag="ACHKUN_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663258.1"
                     /translation="VANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEA
                     IMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLID
                     QRVQAVMLSMRRDGGMLSG"
BASE COUNT          195 a          339 c          209 g          201 t
ORIGIN      
        1 gacaccgtca gcgacgggcg ggcactgctg cccttcccgt tcatctcaaa gccactgccg
       61 tcaatcgggt atgcctggta cttccgcccc tgccaggtca ccggctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcacca ccctgggcct tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca ggctttcccc gtgaatatgc
      241 tgcatcagtt caccacctgc tcaatcgtac agctgaaatc actgtaccgg acgttatccg
      301 tgacgctcca ctcccggcac accaccctca ccgtccggtt atgtttcggc ggtcgccaca
      361 aaaaggcacg gtaaccacca tgccaggata aaaattcatc cagccagcgc cgggttgact
      421 catccgtcac ccggaacacc gcctgaaacg tcttcagttg aggattcagt cctgtggggc
      481 gacgctgttc ataaccgtca ccaaaccgta ccctcgccac cgacggtttc tcactcacct
      541 gcatcccttc acgcgggacc agatgcagcg tttttatctc agccactcag cattcctccg
      601 tcacgtcgca tggacaacat caccgcctgc acccgctggt caatcagctg cacaagactg
      661 cctgccgcct ccggccctat ctggccatta gtcccgtcat tctgaatggc gatatggtag
      721 accggggaat acaccaggcc cgcactaccg ttcatactgc ccaccgctcg cacacccagc
      781 gagccatccg ccgcccgggt caggggcata atggcttcag gaccggcctc ccccatcagt
      841 cccgcccctt ttgcaaaggc aaagtacgtg ggcgtatcca caatactgtt gctgtacgca
      901 ctcaggtttg ccgaggtata cacgccgcct tttgcattgg ccac
//
LOCUS       DAZFRJ010000514          937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000514 DAZFRJ010000000
VERSION     DAZFRJ010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.518"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..351
                     /locus_tag="ACHKUN_005216"
     CDS             <1..351
                     /locus_tag="ACHKUN_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6663259.1"
                     /translation="IVGSFFTAGATMALWGSALAAGGFSATTMLFSLGASMILGGVAQ
                     MLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRD
                     EGGGGKVVVIGRQG"
     gene            597..>937
                     /locus_tag="ACHKUN_005217"
     CDS             597..>937
                     /locus_tag="ACHKUN_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663260.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRV"
BASE COUNT          231 a          218 c          307 g          181 t
ORIGIN      
        1 atcgtgggct ctttcttcac cgccggcgca acgatggcgt tatggggttc agccctggca
       61 gccggcggtt tttctgccac cacgatgctg ttttcactgg gggccagcat gatactgggc
      121 ggtgtggccc agatgctggc cccgaaggca aagacgccgg agtacaaaag tacggataac
      181 ggtaaacaga acacgtattt ttcgtcactg gacaacatga ttgcccaggg gaacccgatg
      241 ccggtgcctt acggtgaaat gctggttggc tcccggcgaa tctcccagga catcagcacc
      301 cgtgatgaag gtggtggcgg gaaggtggtg gttatcgggc ggcaggggta aagcataaaa
      361 aaatcccgca gtgtatggag gctgcgggaa cagaaaatga agattaacca cagggggttt
      421 tgtttttatt ggcccgaaaa aactgtaacg cccgggaatg atatctgcca cggggcgtac
      481 agaaaatgtg aagaaattca gaaattttat tccgtcatga cacaggcacc ctccggggtg
      541 cctgtcgttt ttggggcata aacagattca gacatcagac aggagagggg gacagagtgg
      601 gtaaaggtgg cggcagggcg cacacgccgg tagaggcaaa ggacaatctt aagtccacgc
      661 agatgatgag cgtgattgac gccattggtg aagggccgat tgaaggtccg gtgaaggggc
      721 tgcagagtat tctggtgaac aaaaccccgc tgacggacac ggacggtaat cctgtgatac
      781 atggtgtgac agcggtctgg cgcgccgggg agcaggagca gacaccgccg gaaggttttg
      841 agtcatccgg ctctgaaacc gcactgggcg tggaagtgac gaaggcaaag ccggtgacgc
      901 gcaccattac gtccgcgaac attgaccgcc tgcgggt
//
LOCUS       DAZFRJ010000515          935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000515 DAZFRJ010000000
VERSION     DAZFRJ010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.519"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..224
                     /locus_tag="ACHKUN_005218"
     CDS             <1..224
                     /locus_tag="ACHKUN_005218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663261.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHKUN_005219"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHKUN_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            709..>935
                     /locus_tag="ACHKUN_005220"
     CDS             709..>935
                     /locus_tag="ACHKUN_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6663262.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          247 a          249 c          234 g          205 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
      841 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
      901 cgtaagcatc gggaaggtaa acgcgtgcat cccac
//
LOCUS       DAZFRJ010000516          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000516 DAZFRJ010000000
VERSION     DAZFRJ010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.520"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..582
                     /locus_tag="ACHKUN_005221"
     CDS             <1..582
                     /locus_tag="ACHKUN_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6663263.1"
                     /translation="VNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
BASE COUNT          223 a          216 c          312 g          181 t
ORIGIN      
        1 gtgaacacgg cagcggaagc catccgggcg ctgtcgttac aggtgccggg attccgccgt
       61 cagatgaacg aaggctggta ccagatacgt attcgcggtg aggacacggc accggaggcg
      121 gtgtacgccc gtcttcacga acctctgggt gagggggcgg tcatccatat tgtgccgcga
      181 ctggccggag ccggaaaggg cggactgcag attgtgctgg gggcggcagc catcgtgggc
      241 tctttcttca cggccggagc atcgatggca gcctggggtg cagcgctgag tgccggcggt
      301 ttttctgcca ccacgatgct gttttcactg ggtgccagca tgatactggg tggtgtggca
      361 cagatgctgg ccccgaagcc aaaaacaccg gaatacaggg caacggataa cggtaaacag
      421 aacacgtact tttcgtcgct ggataacatg attgcccagg ggaacccgat gccggtgcct
      481 tacggtgaaa tgctggttgg ctcacggcga atctcccagg acatcagtac ccgtgatgaa
      541 ggcggtgacg ggaaggtggt ggttatcggg cggcaggggt aaagcataaa aaaatcccgc
      601 agtgtatgga ggctgcggga acagaaaatg aagattaacc acagggagtt ttgtttttat
      661 tggcccgaaa aaactgtaac gcccgggaat gatatctgcc acgggggcgt acagaaaatg
      721 tgaagaaatt cagaaatttt attccgtcat gacacaggca ccctccgggg tgcctgtcgt
      781 ttttggggca taaacagatt cagacatcag acaggagagg gggacagagt gggtaaaggt
      841 ggcggcaagg cgcacacgcc ggttgaggca aaggacaatc ttaagtccac gcagatgatg
      901 agcgtgattg atgccatcgg tgagggaccg gt
//
LOCUS       DAZFRJ010000517          915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000517 DAZFRJ010000000
VERSION     DAZFRJ010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.521"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..251
                     /locus_tag="ACHKUN_005222"
     CDS             <1..251
                     /locus_tag="ACHKUN_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shufflon protein C"
                     /protein_id="HFO6663264.1"
                     /translation="WRSTEIKFTTQTYNIGKNIRNFRLGVHAYCAWTYLNGSPFGGFQ
                     QVYSDKNNVWYVNNYAWGNYESGGTISVTCLNIPGAGI"
     gene            complement(539..>915)
                     /locus_tag="ACHKUN_005223"
     CDS             complement(539..>915)
                     /locus_tag="ACHKUN_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663265.1"
                     /translation="WAGFESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAY
                     PGCKLPDLRGVFIRGWDNGRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYW
                     AQGSTTEMRPKNIAFNYIVKAS"
BASE COUNT          236 a          204 c          240 g          235 t
ORIGIN      
        1 tctggcggtc aactgaaatt aaattcacaa cgcagaccta taacatcgga aaaaatattc
       61 gtaatttcag acttggtgtc catgcatatt gtgcatggac gtatcttaat ggttctcctt
      121 tcggtggttt tcagcaggtt tattccgata aaaataatgt ctggtatgtc aataattatg
      181 cgtggggtaa ttatgagtca ggtggaacga tatctgttac gtgtctcaat atacctgggg
      241 ccggtattta attaagcgtc aggcagtagc cagcgtgcca atccggtgtc tggaagaata
      301 atatcagcga tgcactggtt tttgattatt cggcctgtta tgacgcagaa gaagactcca
      361 gaaaaccggt ttatgcgaac tggggtagtg gctcaaacgg ctttgccgtc tgtgacggca
      421 aagcaggcta tggtgcggca ggattcagag cgtacagagg taaaccgggg tattcgggaa
      481 gcatggtctg ggtcaaaata cgttgctgta agttaaaagt taatacgcct taaatacatc
      541 agcttgcttt gacgatgtag ttaaaggcaa tgtttttggg gcgcatttcc gttgtgcttc
      601 cctgggccca gtaaggcata ccgctgtgat gccctttcag ccatccccca cgctcatgat
      661 agacaccgga ttgatcggcc tgataactca ggagatgtct gttccgatct ccatccagcc
      721 cacgtccgtt gtcccagcca cggataaaaa caccgcgcaa atccggcagc ttacacccgg
      781 gataagcacg ggcaagctga ggataccggg aacatgaaaa tgactgcccg ttcatcacca
      841 ggtagccttg tggaggggtg gctgatggcc acggtatagg gcttccgaca gggtaggatt
      901 caaaacctgc ccaca
//
LOCUS       DAZFRJ010000518          912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000518 DAZFRJ010000000
VERSION     DAZFRJ010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.522"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..488
                     /locus_tag="ACHKUN_005224"
     CDS             <1..488
                     /locus_tag="ACHKUN_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO6663266.1"
                     /translation="GAGAIEVDKNLTFRIRGLNNIHVLDCFVNVALEPADGVVDFGKI
                     NSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKI
                     FHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITY
                     N"
     gene            complement(531..731)
                     /gene="glgS"
                     /locus_tag="ACHKUN_005225"
     CDS             complement(531..731)
                     /gene="glgS"
                     /locus_tag="ACHKUN_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO6663267.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          272 a          199 c          174 g          267 t
ORIGIN      
        1 gtggcgcagg agctatagaa gtagacaaaa atttgacctt caggattcgt ggattaaata
       61 atattcatgt ccttgactgc tttgttaatg tcgcactgga accagctgat ggcgttgttg
      121 actttggtaa aataaattcc cgaacaatta aaaataccag cgttagtgag acgtttagtg
      181 tagtcatgac aaaagatcct ggtgcggcct gtactgagca attcaatatt ttagggagct
      241 ttttcactac ggatattttg agtgattata gccatctgga tatgggtaat ggactgctat
      301 tgaagatatt tcataaagat ggaacagcaa cggaatttaa ccgcttctca caatttgctt
      361 ctttttcatc gtctagtgca ccttcggtca ccgcaccatt cagggcagaa cttagtgcga
      421 atccggcaga gactgttgtt gagggaccgt ttagtaaaga cgtcatcctc aaaatcacct
      481 ataactagca tctaatacaa acactaaaac gggccatcag gcccgttata tcagtgctct
      541 aactccagct ctcttgcctg catcatctgt tgacaatacc aggcataacg tgcgcaaaac
      601 caggttctat gttcttcatc catgttacca gtaacggcaa gaatatcgac cgggcgacct
      661 tctgcgtgca ttctggcaaa actacgcgcc aggaaatcga aattatttaa agaattaaga
      721 ctatgatcca taagcacatc ctccggttat cgcatacctt ttaaggccat atgctttttc
      781 tgatttcagt attccccaat cagtgtagtg cctttaaaca taacgtgcgt aaatatattg
      841 tccccgatca cactttttag tgaaaaataa aaaaccatat taaaaaactt tctcttccag
      901 aacttctcta ac
//
LOCUS       DAZFRJ010000519          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000519 DAZFRJ010000000
VERSION     DAZFRJ010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.523"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..629
                     /locus_tag="ACHKUN_005226"
     CDS             <1..629
                     /locus_tag="ACHKUN_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO6663268.1"
                     /translation="TPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELMVSIRP
                     MQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEMLENMD
                     LLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMNSNVCM
                     LYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            660..782
                     /locus_tag="ACHKUN_005227"
                     /pseudo
     CDS             660..782
                     /locus_tag="ACHKUN_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
BASE COUNT          286 a          144 c          208 g          270 t
ORIGIN      
        1 taacgcctta tattttacct ggggttagtt ttttgtctga cattcctcaa gaaaccttgt
       61 ctgagatacg taatcagact attcgtggag aagctcaaat aagactgggt gagttgatgg
      121 tgtcaatacg acctatgcag gtaaatggat attttatggg aagtcttaac caggatggtt
      181 tatcgaatga taatatccag attggccttc aatatataga acatattgaa cgtacactta
      241 atcatggtag tttgacaagc cgtgaagtta cagtactgcg tgaaattgag atgctcgaaa
      301 atatggattt gctttctaac taccagttag aggagttgtt agataaaatt gaagtatgtg
      361 catttaatgt ggagcatgca caattgcaag tgccagagag cttacgaaca tgccctgtta
      421 cattatgtga accagaagat ggggtattca tgaggaattc aatgaattca aatgtttgta
      481 tgttgtatga taaaatggca ttaatacatc ttgttaaaac aagggcggct catcctttga
      541 gcagggaatc aatcgcagtt tcaatgattg taggaagaga taattgtgct tttgaccctg
      601 acagaggtaa cttcgtttta aaaaattaag atagcaatgg cgggttaact tttcgtaaaa
      661 tgaaattaat tctcggtacg gcatgtaaaa cgcggcacct cgtatgcaag aacgtactgc
      721 ggttggttgg tgaactttcg atagtgctag tattggatga tttccagccg ttaccgattt
      781 tatataactt cagtggaatt attcaacaca caggcaaaac ggaataagct gagaaaaaaa
      841 gcgtgctttc ccaatctggg gtaaatcacg tctgaatgac acctgaaaac aggtggtggc
      901 ctcaagaa
//
LOCUS       DAZFRJ010000520          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000520 DAZFRJ010000000
VERSION     DAZFRJ010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.524"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            548..>907
                     /locus_tag="ACHKUN_005228"
     CDS             548..>907
                     /locus_tag="ACHKUN_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFO6663269.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNS"
BASE COUNT          254 a          192 c          193 g          268 t
ORIGIN      
        1 cggccttcgc accaagttac taaattcctt acaatacagt tagttagacg attaaatctg
       61 tctcttttgc ctgtaagaaa tgctctgtta tgtcccttga aaaagccgct gtggttgata
      121 gtggtaattg ggatgttaac tcatagcggc gtgtcgttcc aagcgtcaaa cacgtactga
      181 ctcaacttga aagctatgac gtagccaccg tctctaaaca atcccaaaac ttttagtgca
      241 gcgcatttta gcccgatgcc cccattctgt cgccgtcgcg agacgccgca ggggataaac
      301 aggtttatgg tggtcacggt acttgcagac catatgcaag ctctgccagc ggttcggcct
      361 cgctcccctt gggtacatgg tcatcctgat aagcctcact aactgacata accctgatga
      421 agtcgcaaga ggctccgctt gttgtcttac caccaacggg gctttatgga ttctattgct
      481 taatatatga ttgtatcaaa cttagactgc ataatttaaa cgaacataac aatcaggcag
      541 gggaagaatg ataaagccat tttctgtgag gtatggtcat gttgacgtta gagagcatgt
      601 ccagctgaat gatcttaatt ctgatacacg catggcattg tggaattgct tatatctttt
      661 cctttggact aacaatcgtc aaactgctac agcaacaaaa tgtgcgcagt ctgtgtggat
      721 atattatcta aatcaacctg cggataatat tcctcgttat gaaagtggat acaaaagtga
      781 taagacgttg ctgactgcta tccgagatta tatatatggg gaagcatggt atttggtata
      841 tgatttaatt gagttcatca ttgagagaac taattcatat attaatctat cgaagcatct
      901 caattcc
//
LOCUS       DAZFRJ010000521          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000521 DAZFRJ010000000
VERSION     DAZFRJ010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.525"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..890
                     /locus_tag="ACHKUN_005229"
     CDS             <1..890
                     /locus_tag="ACHKUN_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663270.1"
                     /translation="HDEIGINFKDLPGPLKNALSNSNIPTTVSTAASTIASATTSTVT
                     TASKDPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRP
                     GEADFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGAVGGFAVSRFT
                     GTDPMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGTTLGLADLNKAETGEGRFT
                     DYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGSP
                     HHNPSNYVDTFDIIQETRV"
BASE COUNT          278 a          194 c          205 g          228 t
ORIGIN      
        1 tgcatgatga aataggtatt aattttaaag accttcctgg tccattaaaa aatgcattaa
       61 gcaacagcaa tataccaacc actgtatcga ctgctgcatc cactattgca tcagccacta
      121 cttcgacggt aaccaccgcg tcaaaagacc caataccatg gtttggatta acagctcaag
      181 tagttcgtaa tcatggtgtg gaacttccta tagtcaaaac tgaaaatgga tggaagcttg
      241 ttggagaaac tcctcttact cctgatggcc ccaaagcaaa ttatactgaa gagtgggtga
      301 tcagaccggg agaagcagat tttaaatatg gtgcatctcc actacaggca actctagggc
      361 tggagtttgg cgcacatttc aagtgggatt tagataaccc taatactaaa tatgccgttc
      421 ttaccaatgc tgccgcaaat gcgcttggtg ctgtaggggg atttgcagta tccagattta
      481 ctggtacaga tccaatgtta agtcctcata tcggtgcaat ggttgggcaa gcagcggggc
      541 atgccataca gtataatacc cccggattaa agccagacac tattttatgg tgggcaggta
      601 cgactctggg gctggcagat ttaaacaaag ccgagacagg tgaaggcaga ttcactgact
      661 atcctcgtat atggtggcac gcaagagaag gtgccatctt ccctaataaa gaagatattg
      721 cccgtgcaac aggggctgat atacgagcta tggaagaagg tatacccgtt ggacagcgac
      781 atccaaatcc agaggatgtg gtcatcgata tcgaaagcaa tggctcacca catcataacc
      841 catcaaatta tgttgatacc tttgatataa tccaagaaac aagagtctaa gctttaactt
      901 gttta
//
LOCUS       DAZFRJ010000522          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000522 DAZFRJ010000000
VERSION     DAZFRJ010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.526"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHKUN_005230"
     CDS             <1..198
                     /locus_tag="ACHKUN_005230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663271.1"
                     /translation="KTKRKNKMADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAW
                     RDDYEAGKNPVEAYCDEWGDE"
     gene            195..692
                     /locus_tag="ACHKUN_005231"
                     /pseudo
     CDS             195..692
                     /locus_tag="ACHKUN_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
BASE COUNT          273 a          160 c          254 g          213 t
ORIGIN      
        1 aagacgaaga ggaagaacaa aatggctgat tttggttcaa ctaaatacag cgtcagtttt
       61 gaagaatggc atgaactgtt aatggaatat gcagagttac gtggtggcag tgctgctgat
      121 gctgaagcat ggcgtgatga ttatgaagca ggaaaaaatc cggtcgaagc atattgtgat
      181 gagtggggcg atgaatgagc gagattgact atcaggcgct gcgtgaagca gcagagaaag
      241 caactaaagg atgctacatc gtagggcata catcgggcaa tcagcatggg aatataacag
      301 gagtttttgt ttgtcaaaaa tggaaaggag agcccggtgg cgtaattgca gaatgtcatg
      361 ttaactgcct ggttgaaaca gatgttcagg cttatgcaaa cgctgaattt attgctgctt
      421 ttaatccaaa tgttgcgctg gcactactgg atgaacggga agcccaaaac aaacgaattg
      481 cagagctgaa agctaatctt gtggcgctgg cggcggagaa tgcggtaata aagtctgcaa
      541 ttccagaacc acgggatatt gaggacgaca atgacaatat ggatgacgta tctcttgctg
      601 aagatttcgg gttcaatcat gcaatagaac ggatgaggag acggattcct gaaactccgg
      661 ccaccgacgc tttcctggct gaagtacggg cggggttgtt taacgagctt tgcgcggcgt
      721 ttgtcaggta cgaaaaagtt gcaggactgg atgatagcga tacagtaacg cttaaggaag
      781 cgacagaagc attgctggat tgtgcggaac agcttatcgc gcctgaataa ttaaatttag
      841 ttaaaaattt aatccttaac cggagggatt tctgcaccct caaaacatca ggaggccgcc
//
LOCUS       DAZFRJ010000523          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000523 DAZFRJ010000000
VERSION     DAZFRJ010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.527"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..152)
                     /gene="hha"
                     /locus_tag="ACHKUN_005232"
     CDS             complement(<1..152)
                     /gene="hha"
                     /locus_tag="ACHKUN_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6663272.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRL"
     gene            complement(178..552)
                     /gene="tomB"
                     /locus_tag="ACHKUN_005233"
     CDS             complement(178..552)
                     /gene="tomB"
                     /locus_tag="ACHKUN_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO6663273.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          264 a          183 c          176 g          272 t
ORIGIN      
        1 aggcggtgat ctgcggctga gtaaaatacc gccagttcat tatctgataa ttcgtattta
       61 tttttctcga taacacgctc cagcgtgtca attgtctggc aacgacgtaa acgcattaaa
      121 taatcggttt tcgttaaagg tttttcggac atacttctac ccatggttgt aataattcta
      181 acaagataaa ctcgcaggat tctctttcgt cgcattgaca aaacaacgga atagtcgatt
      241 acctgacttc cgccatttct gaagatcctg catattaata ccataactgc tgaacaacat
      301 aaaggtgtca tccagatatt cgtcgatctg ctcaatgagc ttattgtctt cattatactt
      361 aattttgtaa ttaagtgcga aggtcgcaat atgctcaatc agttcattca actggaggtt
      421 gatcgccgag gttgggtcgt taacccagcc atgattgctt tcttcaaggt ttgcaagaca
      481 gtcatgatac agggtttcac agagaaactt aagctgtgcg atatcatgtc ttttgggtga
      541 gtattcatcc ataacgcgtc cccttcttag tggttgaact aacggacacc tttcgggatg
      601 gaaaaaactt actgacctgg acttgccctc tcgtttgtta gcttaactat aagccactct
      661 ttgcaggttt tcatcgcatt attacgaaaa attacaatta ggaaggaatc gggacaggac
      721 tcactttttc atattagcac taacccgcag caggtaaaag cagtttgctc gaactgttca
      781 taacatttca ggattatcgc cagatgaaat tggtgacact aataccagga ttgctctgaa
      841 tatgacgtaa taaccgagga atgaataaag aattaccgca aataattaag aatag
//
LOCUS       DAZFRJ010000524          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000524 DAZFRJ010000000
VERSION     DAZFRJ010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.529"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..330)
                     /locus_tag="ACHKUN_005234"
     CDS             complement(<1..330)
                     /locus_tag="ACHKUN_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS regulator Mpc"
                     /protein_id="HFO6663274.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTF
                     QQHVQIIE"
     gene            514..816
                     /locus_tag="ACHKUN_005235"
     CDS             514..816
                     /locus_tag="ACHKUN_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO6663275.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMIPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
BASE COUNT          318 a          148 c          163 g          257 t
ORIGIN      
        1 ttcaataatc tgcacatgct gctgaaaagt attcattaga gaaaacacat caatattggt
       61 taacaaacaa cttaagcaca atttgttatc gtagacaaaa atatgttcaa tcactccctg
      121 cgtccgttca ggggcaaggg cattgaaaaa agaaaaaaac gcattttcgt tatactctaa
      181 ggcaaacgta ataaacacat tactatcaat tatttcaata aaaaaagaaa aaccattaaa
      241 atcaatttcc acacgattat caaacaaata aaaatcattt cctaataatc ttaaaaactc
      301 ttctacgttt cttttaatta aaagattcat atgcaggctc tgaagtaagt atatgtgtgg
      361 atattattgc ttgaaaaagc gtattggata atatatatag tatatgtact ttatgtacat
      421 tgcaactatt taaataatat aatagttgcc catgggataa tttggttatt gataaccaga
      481 caaatgtcta aattagacaa tggagaaaag ttaatggatg cagcaaattt aagtccttct
      541 ggcggagtat taccactggc agccacaatc aatggaaatg tttcggtgga tgaaaataca
      601 ggagtaatga taccagaaaa tggggtaagt cgtaatatca gaattgcagc cggaattgca
      661 ctcgctacta cagctctggc tgctttaggt actggtatcg caatggcatg cacaacagat
      721 gcaagctccc aagaattctt aggtttaggt attgctaccg gtgtattagg tggtgttact
      781 gcgcttggcg gcggattagc gatgaaatat gcctaatcat cgcatcgcat aaataaaata
      841 gagaggtcat ggatgcatta tgccattacc tctctcatga aaagag
//
LOCUS       DAZFRJ010000525          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000525 DAZFRJ010000000
VERSION     DAZFRJ010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.530"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..184)
                     /locus_tag="ACHKUN_005236"
     CDS             complement(<1..184)
                     /locus_tag="ACHKUN_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6663276.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHKUN_005237"
     CDS             complement(184..399)
                     /locus_tag="ACHKUN_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6663277.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..>869
                     /locus_tag="ACHKUN_005238"
     CDS             542..>869
                     /locus_tag="ACHKUN_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663278.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNN"
BASE COUNT          244 a          232 c          170 g          223 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataaca
//
LOCUS       DAZFRJ010000526          863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000526 DAZFRJ010000000
VERSION     DAZFRJ010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.531"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>863)
                     /locus_tag="ACHKUN_005239"
     CDS             complement(<1..>863)
                     /locus_tag="ACHKUN_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6663279.1"
                     /translation="TRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNT
                     VGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSIT
                     DVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGA
                     GIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITS
                     GGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIV
                     KTVGKAFPRDSR"
BASE COUNT          185 a          239 c          196 g          243 t
ORIGIN      
        1 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
       61 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
      121 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
      241 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
      301 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
      361 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
      421 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
      481 ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg
      541 atttcattac tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga
      601 ccgttatcaa tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg
      661 aaaaaatcca gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc
      721 gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata
      781 ttgatactgg cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc
      841 tgcctgatat cggtaatccg cgt
//
LOCUS       DAZFRJ010000527          862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000527 DAZFRJ010000000
VERSION     DAZFRJ010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.532"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..670
                     /locus_tag="ACHKUN_005240"
     CDS             <1..670
                     /locus_tag="ACHKUN_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663280.1"
                     /translation="QRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITE
                     MLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAV
                     ANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVR
                     AVGSMNGSAGLVYSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGG
                     MLSG"
     gene            663..>862
                     /locus_tag="ACHKUN_005241"
     CDS             663..>862
                     /locus_tag="ACHKUN_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663281.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLS"
BASE COUNT          195 a          195 c          304 g          168 t
ORIGIN      
        1 gcagcgtgcg gactggcaga aaggcttgca ggaggggctc agtaactggg tggacaatgc
       61 atccgattac gcttcacagg cagcacagct tgcgacggag ggtatctcag ggatggtgaa
      121 taacatcacg gagatgctga acggaaacaa agtggaatgg cgcaactggg cctcatccgt
      181 actgcaggaa atctcaaaag ttcttatgaa tgccgcgatt gtcaacggaa ttaagacggc
      241 ggcaaacggt atgtccggtg cgggaggatt tattggcagc attggtgact ggctgggcgg
      301 tgcggtggcc aatgcaaaag gcggcgtgta tacctcggca aacctgagtg cgtacagcaa
      361 cagcattgtg gacacgccca cgtactttgc gtttgcaaaa ggggccgggc tgatggggga
      421 agccggacct gaagccatta tgcccctgac ccgggcggcg gatggctcgc tgggcgtacg
      481 cgcggtgggc agtatgaacg gcagtgccgg tctggtgtat tccccggtct accacatcgc
      541 cattcagaat gacggggcta acggacagat aggggcggag gcggcaggca gtcttgtgca
      601 gctgattgac cagcgggtgc aggcggtgat gctgtccatg cgacgtgacg gaggaatgct
      661 gagtggctga gataaaaacg ctgcatctgg tcccgcgtga agggatgcag gtgagtgaga
      721 aaccgtcggt ggtgagggtt cggtttggtg acggttatga acagcgccga ccgacgggac
      781 ttaatgccag actgaagacg tttcaggcgg tgttccgggt gacggatgaa ccaacccggc
      841 gctggctgga tgaattttta tc
//
LOCUS       DAZFRJ010000528          860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000528 DAZFRJ010000000
VERSION     DAZFRJ010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.533"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>860
                     /locus_tag="ACHKUN_005242"
     CDS             <1..>860
                     /locus_tag="ACHKUN_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663282.1"
                     /translation="ATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIFS
                     HEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKDTGIIDLSNNQVLGNVHLD
                     MRENPPILHGEKSYNSGKNIGSHPDMTDREIMESGAYNFACTKLSDAGEPDIFGWGDK
                     DVCNSMELSESQLANGYSNRQWPSQSPVIPEGIYNTVYLGYGETLRANTEVTLSSTGY
                     FYEIARAYTDDELSEPLLTVCNQRSSVIGGKEPLTIIIPALEHGMLGSNLHMIERFKN
                     YNFSISGGKG"
BASE COUNT          299 a          138 c          171 g          252 t
ORIGIN      
        1 gagccaccac acaaattgaa tatatatcaa cagcgataga tgtatctttt tcatctataa
       61 atactgcttt atcaataggc gcaatcgcat atccaccttt ggcaatagct atagttccga
      121 taactatctt ttctcatgaa gtaaaaaact atgctgttta tgtgaatcaa attaatgagc
      181 gacataagtt atggcttgaa gctgaaaaat atctggacaa tggaagcgca aaggttctaa
      241 gcattaacaa agatacaggc ataatcgatt tatcaaataa tcaggtgtta ggtaatgttc
      301 atttagatat gagagaaaac cctccgatat tacatggtga aaaatcatat aatagcggga
      361 agaatattgg aagtcatcca gatatgactg acagagaaat tatggagtct ggagcataca
      421 attttgcatg tacaaaactc tcagatgcag gtgaacccga tattttcggt tggggagata
      481 aggatgtatg taattcaatg gaactgtctg aatctcaatt agctaatggg tattctaacc
      541 gccaatggcc ttcacaaagt ccagttattc ctgaagggat ttataataca gtatatcttg
      601 ggtatggaga aacattgcga gctaatacag aagttacatt gagtagcact ggttactttt
      661 acgagattgc aagggcatat actgatgatg aactatctga gccattatta acagtatgta
      721 accaacgtag tagtgtaatt ggtggaaaag aaccattaac aataatcatt ccagctcttg
      781 aacatggtat gcttggctct aatctacata tgattgaacg ttttaaaaac tacaattttt
      841 caatatcagg agggaagggc
//
LOCUS       DAZFRJ010000529          857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000529 DAZFRJ010000000
VERSION     DAZFRJ010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.534"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>857
                     /gene="rfaL"
                     /locus_tag="ACHKUN_005243"
     CDS             <1..>857
                     /gene="rfaL"
                     /locus_tag="ACHKUN_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO6663283.1"
                     /translation="FLFGSVAILTIAALLSLLQSPDAGASMKEIFKAIIENTLLCTIA
                     IPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFADYRHRSISDS
                     MVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVIGLIWILMFK
                     QWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENP
                     VIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCA
                     ILKECIKNS"
BASE COUNT          250 a          144 c          166 g          297 t
ORIGIN      
        1 catttctttt tggtagtgtt gccatattaa ctattgctgc attgctgtca cttcttcaat
       61 cccctgatgc aggtgctagc atgaaggaaa ttttcaaagc tattattgag aatactttac
      121 tatgcacaat agcaataccg atcatattga gagacgagaa aagagaagat gtcgaaaaaa
      181 tcgttttctt ctcatttatt agtgcgttgg gcttacgctg tttttctgaa ttgattacct
      241 attataagga ctatcaacaa gggataatgc cattcgcaga ttatagacac cgtagcattt
      301 ctgactcgat ggtcttttta ttccctgcat tgttaaatct ctggcttatc aaatcagcaa
      361 aataccgcat ttcttttgtg gttctaagcg ttatttttat ttttctgata ttaggaactt
      421 tatccagagg ggcctggctt tccgtgttag tcattggatt aatatggatt ctgatgttta
      481 aacaatggaa gttactatta gtaggagtaa tggttgccat cattgcattg tcggttattt
      541 tcacacataa ggagatgacc gcaaagctaa cgtataaact tcaacaaact aatagttctt
      601 atcgctatgc aaatggtact caaggcagcg cactcgatct aatattagaa aatcctgtta
      661 ttggttatgg ttacggtaac gttgcatata aagatgtcta taataaacgt gtcattgatt
      721 atccagaatg gacctttaga caatcaatag ggccacataa ttttgcgcta ttcatctggt
      781 ttggcactgg tttattaggg ctggtaagtc ttatgatgct atactgtgca atattgaaag
      841 agtgtataaa aaatagc
//
LOCUS       DAZFRJ010000530          853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000530 DAZFRJ010000000
VERSION     DAZFRJ010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.535"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(393..>853)
                     /locus_tag="ACHKUN_005244"
     CDS             complement(393..>853)
                     /locus_tag="ACHKUN_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6663284.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          206 a          225 c          228 g          194 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ctataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgt
//
LOCUS       DAZFRJ010000531          852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000531 DAZFRJ010000000
VERSION     DAZFRJ010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.536"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..66
                     /locus_tag="ACHKUN_005245"
     CDS             <1..66
                     /locus_tag="ACHKUN_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6663285.1"
                     /translation="AQRCVNHISEPDTEIPPGFRG"
     gene            107..529
                     /locus_tag="ACHKUN_005246"
     CDS             107..529
                     /locus_tag="ACHKUN_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6663286.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            526..780
                     /locus_tag="ACHKUN_005247"
     CDS             526..780
                     /locus_tag="ACHKUN_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663287.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            773..>852
                     /locus_tag="ACHKUN_005248"
     CDS             773..>852
                     /locus_tag="ACHKUN_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663288.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFC"
BASE COUNT          244 a          159 c          240 g          209 t
ORIGIN      
        1 gcgcagcgtt gcgtgaatca catttctgaa ccggacactg aaattccgcc gggcttcagg
       61 gggtaagtgt taatttctgg tcatgaggta attttcagga gggcttgtgg caaaagtttt
      121 tacacaagaa gagcgggaaa aaattaaagg gcaggttgtt gaactcgtac gccagagtgg
      181 gcgcgagacg ttacgacaac tggaaactaa aactggggca acaagatatc tgatgagcgt
      241 tctggccaga gagctggttg ccagtggcga tgtatacaac tctggttacg ggttattccc
      301 gtctgaacag gcgcgtaagg actggcaaaa tgcccgtaaa aagctctcaa gggcaaagcc
      361 gaagaaacca tctgcggttg atccggacct tatctggtca ttaccagatg gcgaaatacg
      421 ccgctacgac aggcgtctga acataatctg tctcgagtgc cggaagagcg aagttatgca
      481 gcgcgtactg gcgttttatc aggggaattt tgaggaggtg gtgcggtgag tgaatcaaaa
      541 tgccaggtta atggcaacaa gatagaacca tgtgcagcac tggcaaagtc ccttgagcat
      601 gatgctgaat acacgatgcg aaaaggtctg ctgatataca aaatctggaa tgagagttta
      661 actcgcggtc ctgattttgt gatgttgcgt tccggtgaat tttctaaatt accagttcgg
      721 gtttcatttt gtccgttctg tggtgaaagt ctgaaaacgt gggagaacag aaatgaatga
      781 aattaaagaa ataccagtag tacgtgatga atatggctgc tggacgcatc ctgaatatga
      841 aaaattctgt ga
//
LOCUS       DAZFRJ010000532          852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000532 DAZFRJ010000000
VERSION     DAZFRJ010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.537"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(51..571)
                     /locus_tag="ACHKUN_005249"
                     /pseudo
     CDS             complement(51..571)
                     /locus_tag="ACHKUN_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            615..695
                     /locus_tag="ACHKUN_005250"
                     /pseudo
     CDS             615..695
                     /locus_tag="ACHKUN_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          237 a          165 c          191 g          259 t
ORIGIN      
        1 tacggtaaaa tgattcagga aaaccggttc agcatagcct aggctgaact tttgaggata
       61 tcaacgaacg gaatttttga acatttatga gtagatcgtt gttgaagggc ttcaatgagc
      121 atgtcaagtt catcaactgc tggtctacga ttacgacggt ttgatttacc aatcaaagca
      181 agtttaagta gatatggacg agcacttttc gccgggtttg atgtgtaatt aattccgtat
      241 ataggtttgg ctgcatccag aacactgcca agataactaa catcgtgact gactgttgct
      301 ggacctgcac cagcgttgtt tctcagcctg caatgttcaa ttacgtcatt ttctgtcagt
      361 tcagatagtt tgatcgcgga gatgtcacta tccataagca gttctagcac atatctttta
      421 gtacggtctg ctttacctcc ggcatttggt catttaaata tttgtgtagt aagtcacgga
      481 ctgtaagtcc gtcaacagca tttgatgatg aaatgccata tagatctaat accatcactt
      541 tctgtgtgcc ccatgtttta gcatgagcat gaacgcggcg gctatctcac gggtgatcat
      601 ccctttggca tactgaaaag tatcgggtgt tactgaaccg tgttgataca tcaactgcac
      661 tggatattga gtggccgata ataccgacgt tataacctat aaatgccaat caggctgaat
      721 gttgaaagga tagaatgaac agcccgacac acaaaaaacg gagtccggct ccggtttttg
      781 tgttgccata taaggcagat gtttgttaca gctatttaag tctggagttc aaattaaaat
      841 agggagtttt gt
//
LOCUS       DAZFRJ010000533          848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000533 DAZFRJ010000000
VERSION     DAZFRJ010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.538"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..847)
                     /locus_tag="ACHKUN_005251"
     CDS             complement(<1..847)
                     /locus_tag="ACHKUN_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663289.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENESAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYP"
BASE COUNT          242 a          125 c          173 g          308 t
ORIGIN      
        1 taggatattg atagaatatt gttggtgcat gaaataaagt tgctgatagt aaatcaccaa
       61 gtatgcgtac ctgggttata tctagtgata atacattact taagaatgct tctgtattta
      121 ttgttggaga aggtgatgta tataaagatg gttgttgaga ctgtgaaggt gatttgttat
      181 agtcgccatt aaaaaataat gcctgtatga gcaagtgaat gctgattccg cctccggctc
      241 tgaacgcata tggaagtatc tgttcgattt tattgtgtat ggccatatag gctttataaa
      301 aacatcctga ctgattatat acctgatgat attgatcgtt cagaactctc ataagatgga
      361 gaacagtttc tgcactctca ttctcttttg gtataatttc atgaatgatg ctgtccagtt
      421 gatatttttg cttgcatgtt aggatttttg taattctttc atcgatttca atgcatattg
      481 aattattttt atcggataat aacaaatcat tttcctgtgt atttgtttgt atctcttgtg
      541 ggatattaat taccacatcc gtacatgttc tatccacctc atttgtcttt atgttgtcaa
      601 gaaaatcatt taacgtcagt tgagaaccca attcattaat gtagtccaat gatgacgtag
      661 aagagctatt atcaagaaaa tcgttcagag tgagctcgga gtcgtttctt gttatcggat
      721 atacatttgt ggaagcggct aatttaatac tccggttgct ggaggtagaa gctgttggtt
      781 cagaggttga cgaacactgc atgtcaatgc atacataacc tttatttgaa gttgaatttg
      841 gaatcaag
//
LOCUS       DAZFRJ010000534          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000534 DAZFRJ010000000
VERSION     DAZFRJ010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.539"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>845
                     /locus_tag="ACHKUN_005252"
     CDS             <1..>845
                     /locus_tag="ACHKUN_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663290.1"
                     /translation="GQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALT
                     KTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKP
                     KSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKTLTEMQKQE
                     LLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQI
                     RDSFRQKDADLQSQYQTGDISEDFYRQALSQNAQYLSERLKEQEAFYAESDVQRADWQ
                     KGLQEG"
BASE COUNT          232 a          187 c          283 g          143 t
ORIGIN      
        1 ggtcagcata cggaattcaa ccgtctgctg gggctgggta atgaactgct tcagcagcgg
       61 cagggactgg tgaatgtgcc gttacggctg ccacaggcca ctctggatga taaacagcag
      121 agtgccctga caaaaacaga gcgtgagctg gccctgtcca gactgaaagg ggaagaaaaa
      181 gagcgtgtcc ggctggggta tgcggcggat gacctcggtt ttgtgggtga tccgtatcag
      241 gaggcgagac agcgttatat cagtaatgcc ctggaagcct ggcgcaataa cgaggcgaat
      301 aaacccaaat cccggggtgg aaaatcagag acggaaaaag cggaagacag tttttcccgg
      361 ctgctgaagc agcagaaaga gcaactggca ctggtggggc agaatacaga gctggcgaag
      421 ctgaaatacc agactgcgca gggcgaactg aaaaccctga cggagatgca gaagcaggag
      481 ctgctgcgta atgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg
      541 gaagagacac tgaagaatga gaatgcggct gcgcgtgcgt cgaatgatgc tgaactgctg
      601 gggtacgggc agggggaacg agccagagaa cgcatgcggg agttgcagca gatccgcgac
      661 agcttccgcc agaaggatgc ggaccttcag tctcagtatc agaccgggga tatcagtgag
      721 gatttttaca gacaggctct gtcgcagaat gcacagtatc tgagtgaacg tctgaaagag
      781 caggaagcct tttatgccga atcggatgtg cagcgtgcgg actggcagaa agggctgcag
      841 gaggg
//
LOCUS       DAZFRJ010000535          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000535 DAZFRJ010000000
VERSION     DAZFRJ010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.540"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..425)
                     /locus_tag="ACHKUN_005253"
     CDS             complement(<1..425)
                     /locus_tag="ACHKUN_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO6663291.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQG"
     gene            complement(406..>843)
                     /locus_tag="ACHKUN_005254"
     CDS             complement(406..>843)
                     /locus_tag="ACHKUN_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6663292.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          192 a          279 c          212 g          160 t
ORIGIN      
        1 ccctggtacc aggcgtacgc cagcgcacct gtcgccaccg ccagcgaggt gaccccgacc
       61 atcggcaggg tgatcgcacc ggcaagcccc ctgagcatgg ggatcatccc gccgaaggag
      121 tccttcacct gaccgccctg ttgcagcagg atcagccagg gattctgacc accggcaagc
      181 tgcgtggcga tatccgtaaa ctgtgcgggc agggtgcgca tggccgcttt atactgtccg
      241 acggaaatcc cggctttttg cgcagccagc gcctgacggc tcaggccctg ttcaacagca
      301 gtggcggttt ttctggcgtc agtatccagt cctgaaaaat gacgccttac ccggctcatc
      361 tgctcatcga aacggaccgc atccagactc aggtcaataa taagatcacc aaccggctgg
      421 gacatatctc acacctcccg gaatccccgc tgaagccatc attaatgcgg catcatccac
      481 catgacatcc gccacatccg cagacgataa aatatcgccc cctccgtccc caccgaaccg
      541 gacgcctccg gcaagtcctg ccgctttctg catcagcatt ttgtcctcat ccagcctctc
      601 cacctgctct tcctcatgcc gggggacaag cagactgaaa tcagagggat gcatatccgg
      661 atcgcaaaaa aacaggctga gtacagcgta catcagcccg gaaaaatgca tatccagttg
      721 ggtatcctga aaataatgcg tgcggtaaaa atgtcgccag tcggcatatt cggtggatgt
      781 catcccggca agcatggcgc gccagtcagg cctccccatc tctcgcgcca gtctgagggc
      841 aaa
//
LOCUS       DAZFRJ010000536          837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000536 DAZFRJ010000000
VERSION     DAZFRJ010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.541"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..>837
                     /gene="cif"
                     /locus_tag="ACHKUN_005255"
     CDS             16..>837
                     /gene="cif"
                     /locus_tag="ACHKUN_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO6663293.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIEKTL"
BASE COUNT          255 a          160 c          183 g          239 t
ORIGIN      
        1 gtgaatgaat gagatatgaa agacattacc cttccccccc cgacgtccgc gtcctgtctg
       61 acaggggcca tatctgtaaa tactgaagct gtattatctc ccatgcaaca cacttcagcc
      121 ttacatgtaa gagattttgc ttccctgtgc tcacagaacc tcaaagctaa tgtattgcta
      181 aatagtgatg accacgaagt acctatacat cagaaaaatc ctgctgcaat aatgcaaaat
      241 atcgactcta acatcaaaca gatggcaaca gactggggga tgtcgattga ggaggttgag
      301 gttattatag ggcgagagaa aggcattgtg gaaccctcct gcggagttac cgctaatgct
      361 attatgaaac tatttctgga caaggatggc ttcagttact gctttgaaaa tgaacagaca
      421 ctatcgctcg agcagcttca ggagcgcctg tcctgtatgc ctgaatgtaa gagctttgta
      481 ttacgtgtta atgatggtgc gcttggtcat gcttacattg tcgatattcc caaaggagaa
      541 aactcttgtc gtcctgcatt cttgtatcag tcagatttag gagagggcgt caccagaaag
      601 ttaagatttg aggactggat gacgcataaa gcattgactc cgattttgct ggatgatatt
      661 tgtaattact tctcctgcat gtctcaaaat aagacagatt tggagcagat tgcaacgtta
      721 tttgatattg atggaaatgt taaaatgtta cgaaaggaaa atattcaata tcaaaagcat
      781 gacaatttta gtttccagtt gtttgagtat gacaccgata atattgaaaa aacattg
//
LOCUS       DAZFRJ010000537          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000537 DAZFRJ010000000
VERSION     DAZFRJ010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.542"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>836
                     /locus_tag="ACHKUN_005256"
     CDS             <1..>836
                     /locus_tag="ACHKUN_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO6663294.1"
                     /translation="LLSVIGTGRLYITPEGHACHAYFREVSGNGIRFTLTCSGYEGRF
                     WISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDDVQFTMLNKD
                     VYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHSNTIFFDGYIAPGWTDYPV
                     TELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNLFIADVQAADLLCTISNEA
                     VMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCRLIRNNTTIAYGSIVKINE
                     DF"
BASE COUNT          253 a          132 c          184 g          267 t
ORIGIN      
        1 ttgttgtcag tcataggaac cggtcgcctc tatattacgc ctgaagggca tgcttgccat
       61 gcatattttc gcgaagtcag cggtaatggt atccgcttca cgttaacatg ctcaggctat
      121 gaggggcgtt tttggatatc agaagaacag ttcattcagt ggtgccagga gttattcccg
      181 tatagcgaaa gccggctgat accggaagat atgataaaac tcatgatctt atgggtaatg
      241 cagactgctt taccagaagg tgatgtgtct gttgatgatg ttcaatttac gatgctaaat
      301 aaagatgttt atccggtaat agaaaataat aatggagaaa acagacttaa tgtaattata
      361 ctcgaaataa cggtacagag tttacaatat ctaattaatg aaaattggca atttgttcca
      421 cattctaata ccattttctt tgatggatat attgctcctg ggtggactga ttacccggtt
      481 acagagctga ctgttggtga tagtttaagg ctctatcatg ttgatgacag caaagaaaga
      541 gagtgttggc ttgttattaa taacccttta gcaaccgtaa aactttgtga taataattta
      601 tttattgcgg atgtccaggc tgcagatttg ttatgtacta tcagcaatga ggctgtaatg
      661 gatcgcattt attgtgttat tggaacaatt catgttgata tacatatgtt gcgcaatgcc
      721 aaaaaagatg atattatcga ttcaactgga tatcatcttt ttggtgggtg ccggttaata
      781 cgcaataata cgactatagc atacggaagc attgtaaaaa tcaacgaaga ttttta
//
LOCUS       DAZFRJ010000538          835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000538 DAZFRJ010000000
VERSION     DAZFRJ010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.543"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>835
                     /locus_tag="ACHKUN_005257"
     CDS             <1..>835
                     /locus_tag="ACHKUN_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFO6663295.1"
                     /translation="HSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPK
                     TTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQ
                     IQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVKDQTLELT
                     LLRSQHKPGEVVKTDNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVNQAYIMNH
                     PLQIVPVKPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESYGLEISAMF
                     S"
BASE COUNT          248 a          179 c          160 g          248 t
ORIGIN      
        1 tgcacagtga gatccgtgca taccacgaag atgcaggttt tttcgcggcc tgggattttg
       61 cttcaaatta tcgtgatggg gaatcatatg ttttactcgc tgaaaaaatg acaactgtga
      121 tcagcgggcc taaaactacg atgacgctga tttatcacta caattcctct tatcttcgct
      181 tcgcatttac cctgacacag gacagtccac gcgttgatgt ccaaactttt attgattggc
      241 acgaacctaa tgtttctctc aaagtaaaat tcccggtatc agtacaaaca tctcttgcac
      301 aatgtcaaat tcagttcggt gtaattgaca gaccaacgca tagcgatgat tcgttcgctt
      361 ttgccaaaga tgaaatacca gcacatcact gggtcgacct ttctgatcaa gaaactggca
      421 ttgctttaat agcaaaaaac aaatatggct atcgtgtcaa agaccagacg ctagagttaa
      481 ctttgttgcg ttcgcagcac aaacctgggg aagttgttaa gacagataat tatgataatt
      541 tcctgattaa taattttgca gatattggta aacaaaaatt cacattctcc ttatttcctc
      601 atcatggaga ttaccgtgtg ggtggtgtcg ttaaccaggc ctatataatg aatcatccct
      661 tacagatcgt ccctgtcaaa ccacacccgg gtgaactacc cccttcacta tcattttttg
      721 ctatcgatac taacagcgtt atcatcgaaa caataaaatt agctgaagac ggcgacggta
      781 taatcgtaag attatatgag agttatggat tagaaatttc agccatgttt tcgtg
//
LOCUS       DAZFRJ010000539          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000539 DAZFRJ010000000
VERSION     DAZFRJ010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.544"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..746
                     /locus_tag="ACHKUN_005258"
     CDS             <1..746
                     /locus_tag="ACHKUN_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO6663296.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZFRJ010000540          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000540 DAZFRJ010000000
VERSION     DAZFRJ010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.545"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>824
                     /locus_tag="ACHKUN_005259"
     CDS             <1..>824
                     /locus_tag="ACHKUN_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HFO6663297.1"
                     /translation="IEICDNDYAGTMTGGRVLSIDAASRTLTLDREVTLPETGAATVN
                     LINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENT
                     DGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQA
                     KWDTPRVVKGVRFSLRLTSGSGQDSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSY
                     GQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIA"
BASE COUNT          170 a          246 c          260 g          148 t
ORIGIN      
        1 attgaaatct gtgataacga ctacgccggt accatgaccg gcggacgtgt cctgtccatc
       61 gatgccgcca gccgcaccct gacactggac cgtgaggtga ccctgccgga gacaggtgcc
      121 gccacggtga acctgattaa cggcagcggt aagccggtga gcgtggccat cactgcacac
      181 cccgcgccgg accggataca ggtcagcacc ctgcctgatg gtgtggagac atacggtgta
      241 tgggggctct ccctgccgtc actgcgtcgt cgcctgttcc gctgtgtttc catccgggaa
      301 aacacggacg gtacctttgc catcacggca gtgcagcatg tgccggagaa agaagccatc
      361 gtggataacg gggccagctt tgagccgcag tcaggcaccc tgaacagcgt catccctccg
      421 gcagtgcagc acctgacggt ggaggtgagc gcagctgacg gccagtatct ggcgcaggcg
      481 aaatgggaca cgccgcgggt ggtgaagggc gtgcgcttca gtctgcgcct gaccagtgga
      541 agcggtcagg acagccgtct ggtgaccacc gccatcaccg cggatacaga gcaccgtttc
      601 agcggtctgc cgccggggga atacaccctg acggtcaggg cgattaacag ttatggccag
      661 cagggggaac cggccaccac cacgttcagg attaatgcac ctgcggtacc cgccacgatt
      721 gagctgacac cgggctattt tcagataaca gcggtcccgc gtcttgcggt gtatgacccg
      781 acggtacagt ttgagttctg gttttcggag acaaaaatcg caga
//
LOCUS       DAZFRJ010000541          821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000541 DAZFRJ010000000
VERSION     DAZFRJ010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.546"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..651
                     /locus_tag="ACHKUN_005260"
     CDS             61..651
                     /locus_tag="ACHKUN_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6663298.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(749..>821)
                     /locus_tag="ACHKUN_005261"
     CDS             complement(749..>821)
                     /locus_tag="ACHKUN_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6663299.1"
                     /translation="KYRVLLNRVDTSTAQDIEWPALP"
BASE COUNT          255 a          175 c          179 g          212 t
ORIGIN      
        1 tcaaaattat tttctgagat gcattatgat atgaacacca atttcgtata gagtctcact
       61 atgtctcgaa tttttgctta ctgtcggata tcaacgctgg atcagaccat cgaaaatcaa
      121 cgccgggaaa tcgaaagtgc aggttttaaa atcaaacctc agcaaataat cgaagaacac
      181 attagcggct cagcagcaac cagtgagcgt cctggtttta accggttgct tgctcgcctg
      241 aaatgtggtg atcaattgat tgtgacaaaa ctggatcgcc ttggttgtaa tgcaatggat
      301 atcaggaaaa cagtggaaca actgaccgaa acaggtatca gagtgcattg cttagcattg
      361 gggggcattg acctgaccag tccaacagga aaaatgatga tgcacgtaat ttcagcagtc
      421 gctgaatttg aacgagacct tttacttgaa cgcactcatt ccgggatagt aagagcccgc
      481 ggcgcaggga aacgttttgg tcgaccacct gtgttaaatg aagaacagaa acaggtggta
      541 ttcgaacgaa ttaagtcagg tgtaagtata agtgccattg cccgggaatt caaaacctcg
      601 cggcaaacca ttttaagagc caaagcaaaa cttcagacac ctgacatata aaaaataatc
      661 tcggtgtgag atgctttacg tcttccaagc ccccttcctt gccgtaaatg gaaagataca
      721 tctaattata gaatttatat gttttaccct acggcagtgc tggccattca atatcctgtg
      781 cagttgacgt atcaacacgg ttcagcaaca cccgatactt t
//
LOCUS       DAZFRJ010000542          816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000542 DAZFRJ010000000
VERSION     DAZFRJ010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.547"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..659
                     /locus_tag="ACHKUN_005262"
     CDS             150..659
                     /locus_tag="ACHKUN_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6663300.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            631..>816
                     /locus_tag="ACHKUN_005263"
     CDS             631..>816
                     /locus_tag="ACHKUN_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663301.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMN"
BASE COUNT          244 a          165 c          203 g          204 t
ORIGIN      
        1 aaccatgtcc ggtttctctt caagttaact atatgaaaaa tataaaaaca ggtcttctgt
       61 gaaccggaca tgcacaaaaa atagatatgt aaaccggaca tgaccggttt tgttgtgatt
      121 gtgaggtgag agtttttgcg aggtgaggag tggctacgca gactgaagtt gccaggcatt
      181 taagtctgac cgatcgccag cttcgcagat tgcagaaatt gccgggtgcc ccgatatcaa
      241 ataagcgagg gcaactggat ctggatgcct ggcgcgattt ttacatatcg tatctgagga
      301 gaagtaaaaa cgatgtgcct gatggcgata gcgaagacga ctatgaggaa aaattgctta
      361 ttgccagatg ggaactgaca gcagaacagg ctgttacaca gcagttaaaa aatgaggtgt
      421 caaaaggaaa acttattgac accgggttct gtatttttgc cctcagtaag ctggcaatgg
      481 cgttatccag tacgcttgat tccatccctt tatccatgca acgacagttt cctgatttaa
      541 caccgcgcca tcttgaccat ctgaaaaccc ttattgcgaa gggggcaaat cagtgtgcgc
      601 gggcggggga taaattaccg gatttactcg atgaatatat cagagcaaca actgaataat
      661 atgatgagtg ctgtcacaac agcattacag cccctgataa gggcattgcc ggtgacgcca
      721 gttgaatggg ctgatcaaaa ttattatctg cctaaagaat cttcatatgg tgagggagaa
      781 tggaaaacgc tgccattcca gatcgccatc atgaac
//
LOCUS       DAZFRJ010000543          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000543 DAZFRJ010000000
VERSION     DAZFRJ010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.549"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..>806
                     /locus_tag="ACHKUN_005264"
     CDS             71..>806
                     /locus_tag="ACHKUN_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6663302.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLG"
BASE COUNT          266 a          170 c          147 g          223 t
ORIGIN      
        1 aaaaatatat acgcaaaaaa cattgattaa gtgaatatac catggaagaa aaatataacc
       61 ggagtagtgt atggccaatc tctacgactt gaaaaagttc gacttaaacc ttcttgtcat
      121 atttgagtgt atttaccagc atttaagcat tagcaaagct gctgaatcac tatatatcac
      181 cccatcagcc gtgagccagt cgttacaacg cttacgtgcg caatttaatg accctttatt
      241 cattcgctca ggaaaaggta tcgcgccgac gacaacaggg ttgaatctgc atcaccatct
      301 tgagaaaaac ctcaaaggcc ttgaacaaac aattaatata gtcaataagt ccgaactaaa
      361 gaaaaacttt attatttacg gccctcagct catttcttgc tctaataata gtatgctgat
      421 ccgctgccta cggcaagatg cctctgtcga gattgagtgc catgatatcc tcatgtcagc
      481 agagaatgct gaagaactgt tggttcaccg caaagccgat ctggtgatca cccaaatgcc
      541 agtgatcagc cgttccgtca tttgcatgcc attgcataca attcgcaata ctctaatttg
      601 tagtaataag catccgcgaa ttactgacaa tagcacttat gaacaaatta tggcagaaga
      661 gttcacccaa ttaatatcca aatcagcagg agttgatgat attcaaatgg aaatagacga
      721 gaagtttatg aatcgcaaaa tttcatttcg tggttcctct ttgctgacaa tcattaatag
      781 cattgcagtt acagatttac tgggaa
//
LOCUS       DAZFRJ010000544          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000544 DAZFRJ010000000
VERSION     DAZFRJ010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.550"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..215
                     /gene="etgA"
                     /locus_tag="ACHKUN_005265"
     CDS             <1..215
                     /gene="etgA"
                     /locus_tag="ACHKUN_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO6663303.1"
                     /translation="DLLNDACINISVAGYILASNIKSRGNTWDAVGAYNAGYFNTPNA
                     VELRRQYAMKIYKTYTKLKNNEQIID"
     gene            complement(212..>802)
                     /gene="escU"
                     /locus_tag="ACHKUN_005266"
     CDS             complement(212..>802)
                     /gene="escU"
                     /locus_tag="ACHKUN_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO6663304.1"
                     /translation="TLIFYFMGHSYANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFG
                     YLLFSVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLAN
                     NIKKSTVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDI
                     TLARSLYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
BASE COUNT          264 a          148 c          138 g          252 t
ORIGIN      
        1 atgatttatt gaatgatgcc tgtataaaca tatctgttgc tggttatatc ttagcctcta
       61 acataaaatc cagggggaat acatgggatg cagttggtgc gtataatgca ggctacttta
      121 acacaccaaa tgcagtagaa ctcagaagac aatatgcaat gaaaatatat aaaacataca
      181 ctaaattaaa aaacaatgaa caaattatcg attaataatc aagatctatt gctatacgaa
      241 tcaattgtgc tacaggttca aaaaagtctt ctgttatgta ttgtccttta tgtatgttct
      301 tataaagact tcttgccaaa gtaatatctt caatcactgg aatatcatag agttcagcca
      361 gtttaatgat ctgtagagct ttggcatctt ttcctgtttc aataactaaa ggcaatggag
      421 tctccccgag tttatagtat aggcaaatcg caatgtgagt cgggtttttg acgatcacgg
      481 ttgatttttt gatgttatta gccagacttc cactttgtat ctcagaatga aggcgacggc
      541 gctccccttt aatttcaggg ttaccatcag tatccttggc ttctcgtttc acctcatctt
      601 tactcatttt cattttcttc agtccctcat gcttctggaa ccagaaatca aatactgaaa
      661 agagtagata tccgaataac acgcctttcc ataaaagaaa aacaaaaaag gcaacaacga
      721 caagagtttg atatgcgttc agtcttgtga aatttgcaaa ctcatttgca tatgaatgcc
      781 ccataaagta aaaaatgaga gt
//
LOCUS       DAZFRJ010000545          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000545 DAZFRJ010000000
VERSION     DAZFRJ010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.551"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(2..271)
                     /locus_tag="ACHKUN_005267"
     CDS             complement(2..271)
                     /locus_tag="ACHKUN_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6663305.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENVGGMK"
     gene            complement(273..>800)
                     /locus_tag="ACHKUN_005268"
     CDS             complement(273..>800)
                     /locus_tag="ACHKUN_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6663306.1"
                     /translation="GPQGPKGDKGERGDTGPVGATGERGPTGDAGPAGPQGPKGDRGE
                     RGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIR
                     FRLGPASIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQDV
                     KGLDEVGSDTGRTGE"
BASE COUNT          129 a          294 c          179 g          198 t
ORIGIN      
        1 gctatttcat cccgccaaca ttttcccacg tcccgtcagc acgcaggatt tgcagcggtc
       61 ttaccacgca ctgtatctgc tttttatccg catccagtat caccacctgc gtgattaccc
      121 tgtcctgctc cgggataatg ccattctcat ctgactccag gatgtctgcc ggccccagtc
      181 gcagctgtgc tgtaagtaac tccccgtgtt cacagtcatc atgctttccg caaccacaca
      241 gacgctccat aagctttctc aaaatattca tgtcattctc ctgttctgcc tgtatcactg
      301 cccacttcat ccagtccctt aacatcctgc cacggcccgt cgccaaacct gacctgcaaa
      361 tgctgaaaaa aaccctgaac ccgtgtggca tctttggggt caagaaaggt cagtccggtg
      421 atgagtgcgc catctgtatc cgggaaccag ccatggctgt ttgtctcaat aatgctcgcc
      481 ggccccagac gaaaacggat ttgtgtctcc cccgggtcgc ccttcggtcc ctgaggtccg
      541 gttgccccca ccgggccagc cgcacctgtt tctcctttcg gtccctgtgg gccagccggg
      601 cctgccgcac cggtatctcc ctttggaccc tgtggacctg catttcccgt caggccggtc
      661 tctccccgct ctcccctgtc gcctttcggt ccctgtggtc ctgccggacc agcatcacct
      721 gtcggccccc gttcgccggt tgccccgaca gggccggtgt caccacgctc tcccttatcc
      781 cccttcggcc cctgagggcc
//
LOCUS       DAZFRJ010000546          792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000546 DAZFRJ010000000
VERSION     DAZFRJ010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.552"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..>792
                     /locus_tag="ACHKUN_005269"
     CDS             105..>792
                     /locus_tag="ACHKUN_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6663307.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          264 a          133 c          171 g          224 t
ORIGIN      
        1 atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa
       61 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
      121 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      181 tcggtgtaga aatctatgct gctgccaaac aaggtgaacc cgatccagaa ttaaacacat
      241 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      301 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtacaggga cgttatgaag
      361 gctttggtcc taatggctca atgattattg ctgagacatt gacttcaaat gttaaccgta
      421 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      481 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggaaca gaccctgacc
      541 atatttttga aattttgctt gaagctgaag ttgatgttcg tgatgtaact gaagaagaag
      601 gtaatattgt tatttatact gaacctactg accttcataa aggaatcgcg gcgctaaaag
      661 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
      721 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
      781 acgatgtaca aa
//
LOCUS       DAZFRJ010000547          789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000547 DAZFRJ010000000
VERSION     DAZFRJ010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.553"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..>789
                     /locus_tag="ACHKUN_005270"
     CDS             35..>789
                     /locus_tag="ACHKUN_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6663308.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPI
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPVIAAIHREYKQTWKTLD"
BASE COUNT          232 a          185 c          199 g          173 t
ORIGIN      
        1 gttcaccctg aaatctgtaa tgaggtaaaa caaaatgagt aaagtcttta tttgcgccgc
       61 cattccggac gaacaggcaa taaaggaaga aggtgcagtc gctgtagcca ctgccattga
      121 agccggtgat gaacgtcgcg cccgcgcaaa atttcactgg caattcctgg aacattatcc
      181 ggctgctcag gactgcgctt ataaatttct tgtttgcgag gataaacccg gtattccccg
      241 ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg atgaggagtc
      301 tgcttccttt gtcccggttg agactgaatc agatccgata aacgtcactt ttgacaagct
      361 ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt
      421 tgatatggtg attagcgcgc aggaactgtt gcaggaagac atggcaacat tcgacggaca
      481 tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtatccgg agcttaagct
      541 gcatgccatc gggtgggtta agcataaatg taagccaggt gccaaatggc ccgaaattca
      601 ggcagagatg cgcatctgga aaaaacgtcg cgaaggtgaa cgcaaggaaa ccggaaaata
      661 cacgtctgtt gttgatctcg cccgcgccag agccaatcaa cagcacactg aaaattcaac
      721 aggaaaaatc agcccggtca ttgctgccat tcatcgcgaa tacaagcaga catggaaaac
      781 actggatga
//
LOCUS       DAZFRJ010000548          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000548 DAZFRJ010000000
VERSION     DAZFRJ010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.554"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..>788
                     /gene="nleL"
                     /locus_tag="ACHKUN_005271"
     CDS             72..>788
                     /gene="nleL"
                     /locus_tag="ACHKUN_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO6663309.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLS"
BASE COUNT          233 a          135 c          168 g          252 t
ORIGIN      
        1 acagaaagtt gtcccggcag ttgtgttttc taattttact ttagtggact aagtaaaaag
       61 gagtgagata aatgctgccc actacaaata tctctgtaaa ttctggagta atatcttttg
      121 aaagtcctgt agattcacca tctaacgagg atgttgaagt tgccctcgaa aagtggtgcg
      181 ctgagggaga atttagcgaa aatcgtcatg aggttgcatc aaaaatactt gatgttataa
      241 gtactaatgg agagacttta tcaatcagtg agccaataac aacattacca gacttgcttc
      301 caggttctct gaaagaactg gttttgaatg gatgtacaga gcttaaatca ataaactgct
      361 taccccccaa cttatcttca ttaagtatgg ttggatgctc atcattagag gttataaatt
      421 gcagcatacc tgaaaatgtc attaatttat ctttatgcca ttgtagttct ttgaaacata
      481 tagaaggttc ctttcctgag gcactcagaa attccgtata tttaaatggc tgtaattcat
      541 taaatgaatc gcaatgtcaa ttccttgcat atgatgtcag tcaaggccgt gcctgcctga
      601 gcaaagctga gcttactgct gacttaattt ggttgtcggc taaccgaacg ggtgaagagt
      661 ctgctgaaga attgaattac tctggatgtg acttgtcagg tctaagtctt gtagggctga
      721 atttatcatc agtaaatttt tctggagcag tgcttgatga tacagatctc aggatgagtg
      781 atttgtct
//
LOCUS       DAZFRJ010000549          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000549 DAZFRJ010000000
VERSION     DAZFRJ010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.555"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..104)
                     /locus_tag="ACHKUN_005272"
     CDS             complement(<1..104)
                     /locus_tag="ACHKUN_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663310.1"
                     /translation="MTQTESVILAHTRRCVPAESCGFVVRTPEGERYLP"
     gene            complement(110..>785)
                     /locus_tag="ACHKUN_005273"
     CDS             complement(110..>785)
                     /locus_tag="ACHKUN_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6663311.1"
                     /translation="LNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNEKGEPVTWQ
                     GREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYA
                     RFLDAVNFVAGNPEADPEQELRDRWVVEQMSSLTAMTASFVLATPTETDGALFPGRIM
                     LANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLS
                     INKLSQ"
BASE COUNT          164 a          280 c          184 g          157 t
ORIGIN      
        1 ggcagatacc gctccccctc cggcgttctc accacgaagc cgcacgactc cgcaggcaca
       61 caccgccgtg tgtgtgccag aatcactgat tctgtctgtg tcattggatt tactgcgaaa
      121 gtttgttaat ggaaaggaaa ccgccaaaat tagccaccat gccgcgcatc tcacacccgc
      181 gcatgcactt gctgcatctg tccttacgga tatccgtagt ggggttgtcg aactcatccg
      241 ccaccgcagg gccgttatac ccgcattcat ctccccggta atcccacata caggtgttcg
      301 ccagcatgat gcgaccggga aacagcgccc cgtccgtctc ggtcggtgtg gccagcacaa
      361 acgaggccgt catggccgtc agcgatgaca tctgctccac cacccagcgg tctctcagct
      421 cctgctccgg gtccgcttcc ggattgcctg ccacaaagtt caccgcatcc agaaaacggg
      481 catacacccg gcggcggacc accgtggcac ccaccaggct ctgcaaatcc tccgccatcc
      541 cggtgacaag accgaacaga ttggacaccg tcagcgacgg gcgggcactg ctgccctttc
      601 cgttcatctc aaagccactc ccctcaatcg ggtacgcctg atattcacgc ccctgccagg
      661 tcaccggctc ccctttttca ttcagctcat tgcagaaaaa ataccgctca ccgccctgca
      721 ccgtcaggtc gatttcccag agtaccaccc gcggtgactg ctctgactta accgactcgt
      781 taaga
//
LOCUS       DAZFRJ010000550          783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000550 DAZFRJ010000000
VERSION     DAZFRJ010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.556"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHKUN_005274"
     CDS             <1..321
                     /locus_tag="ACHKUN_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO6663312.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..>783
                     /locus_tag="ACHKUN_005275"
     CDS             487..>783
                     /locus_tag="ACHKUN_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6663313.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHC"
BASE COUNT          173 a          215 c          225 g          170 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgc
//
LOCUS       DAZFRJ010000551          782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000551 DAZFRJ010000000
VERSION     DAZFRJ010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.557"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(36..740)
                     /locus_tag="ACHKUN_005276"
     CDS             complement(36..740)
                     /locus_tag="ACHKUN_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO6663314.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          179 a          209 c          202 g          192 t
ORIGIN      
        1 agcgatgagg cagccttttg tcttattcaa aggccttaca tttcaaaaac tctgcttacc
       61 aggcgcattt cgcccagggg atcaccataa taaaatgctg aggcctggcc tttgcgtagt
      121 gcacgcatca cctcaatacc tttgatggtg gcgtaagccg tcttcatgga tttaaatccc
      181 agcgtggcgc cgattatccg tttcagtttg ccatgatcgc attcaatcac gttgttccgg
      241 tacttaatct gtcggtgttc aacgtcagac gggcaccggc cttcgcgttt gagcagagca
      301 agcgcgcgac cataggcggg cgctttatcc gtgttgatga atcgcgggat ctgccacttc
      361 ttcacgttgt tgaggatttt acccagaaac cggtatgcag ctttgctgtt acgacgggag
      421 gagagataaa aatcgacagt gcggccccgg ctgtcgacgg cccggtacag atacgcccag
      481 cggccattga ccttcacgta ggtttcatcc atgtgccacg ggcaaagatc ggaagggtta
      541 cgccagtacc agcgcagccg tttttccatt tcaggcgcat aacgctgaac ccagcggtaa
      601 atcgtggagt gatcgacatt cactccgcgt tcagccagca tctcctgcag ctcacggtaa
      661 ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc
      721 cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac
      781 ca
//
LOCUS       DAZFRJ010000552          782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000552 DAZFRJ010000000
VERSION     DAZFRJ010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.558"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..275
                     /locus_tag="ACHKUN_005277"
     CDS             6..275
                     /locus_tag="ACHKUN_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO6663315.1"
                     /translation="MNILKKIMQRLCGCGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            388..782
                     /locus_tag="ACHKUN_005278"
                     /pseudo
     CDS             388..782
                     /locus_tag="ACHKUN_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          221 a          139 c          213 g          209 t
ORIGIN      
        1 atgacatgaa catattaaaa aaaattatgc agcgtctgtg cggttgcgga aagcatgatg
       61 accgtgaaga cggggagtta cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtcagatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgtg tggtaagacc gctgcaaatc ctgcgtgctg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaacccga cagcttcaca aaaccggagt
      301 ccggctccgg tttttgttgt catgtaaggc agatgtttgt taaagctatt taagtctgga
      361 gtttaaatta aaatagggag ttttataatg ccgttaaatt cggagattag atcaagctca
      421 tgtttaatgg attgaatgtc cttcgagctc aagtagcatc tagcggtcga ggggagttta
      481 cattaggtaa tgagactgtc agcattgtat ttaatgaaac cgatgggcgt tttctatcca
      541 gcggcagtag tgggggattg cttactgagt tattccttta tgggtttaat aacggccctg
      601 aagctcttcg cgataggatg ctcagtatgc tttcggactc aggtgaagca caatcgcaag
      661 agagtattca ggacaaaata tctcaattta agtttcctgt tagttcagga aatttccagt
      721 gcccgccaga gtctattcag tgtccaatta cactagagag acccgaagaa ggagtgtttg
      781 tc
//
LOCUS       DAZFRJ010000553          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000553 DAZFRJ010000000
VERSION     DAZFRJ010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.559"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>779
                     /locus_tag="ACHKUN_005279"
     CDS             <1..>779
                     /locus_tag="ACHKUN_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO6663316.1"
                     /translation="DADKNIIKKILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLW
                     PDGWNIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSL
                     PDTDKIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVL
                     GEEFINERKLSDKAYIGVYKTTGTGKYDWLNPESIGVNDVTPADESTWCIGKGRCVDD
                     FLFKDVSKLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQK"
BASE COUNT          281 a          133 c          158 g          207 t
ORIGIN      
        1 tgatgcagat aaaaacatta tcaagaagat attaacagaa atatctaaaa tgccagaaga
       61 tagcattttt actaagatca atacaaggat tcctcgagac acaatgccca tccttcgtcg
      121 ttatcaccta tggcctgatg gatggaatat tcgtgggctc aatggattca tgctatcaca
      181 taaaggtagt gaagtgattg atgctgttat cgcaggccag aatcaggctt acagggaact
      241 aagaagaata agagataata ttcatagtga aatatacttc aaacaaactg atgaattgtc
      301 ctcacttcca gatacagaca aaattggagg gattctggta aaaaaatacc tttcaggaag
      361 tcttttttca aaattcagac aagatactat tatcccggag gcattgagca ccctccagat
      421 atcaggtcct gacttgattc aaagaaagat gttgcaattt ttcaggagta gaggggtgtt
      481 aggtgaagag ttcattaatg aaagaaaact gagtgataaa gcttatattg gtgtctacaa
      541 aacaactggc acagggaaat atgactggtt aaaccctgaa tcgatcggcg ttaatgatgt
      601 cacgcccgca gatgaaagta cctggtgtat aggaaaaggc cggtgtgttg atgacttcct
      661 gttcaaagat gtttcaaaac taaaaacaga aaatcttcca gaattattct taacaaaaat
      721 agatactgat acgtttttct ctcagtggtc aaccaaaacc aagaaagatc tgcaaaaaa
//
LOCUS       DAZFRJ010000554          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000554 DAZFRJ010000000
VERSION     DAZFRJ010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.560"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            18..>779
                     /locus_tag="ACHKUN_005280"
     CDS             18..>779
                     /locus_tag="ACHKUN_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6663317.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHE"
BASE COUNT          261 a          154 c          151 g          213 t
ORIGIN      
        1 ttagtgttta ttgacgtatg tactggatta ttaacgataa tatcgagttc tggcctgagc
       61 accgaaaatt aatatcggta cataacgccg atcttaacgt cgttctgaca acgccagcca
      121 gtcgatgttt atcacttcta cttgaagctt ttcctgatgt ggttgcacaa caagattttt
      181 tcaccagagt ctgggaagaa gaaggtatgc gtgtgcctac taacacgtta tatcagaaca
      241 tatccattat cagacgcgga tttcgcgctg ttggtgatac tacccactcg ctaattgcaa
      301 ccgtgccgag aagaggattc aagatccata atgacattaa catacaaaat catgtaataa
      361 actcgtcaac agacgcacat acacacaatg ccccacctgc cataaaagtt aatgcgggat
      421 acaaagagag cattggtggc gcaaagaatt tcaataacaa aatcctcaaa catataaaaa
      481 gtcatctaat tatgttgagc gcatttgtca taggcgcata ttctgcatat tggctatgga
      541 ataataatca accgaagcca tttttcaagg attacaagac tgttgcggaa attaatggct
      601 gccattttaa tgtaacagaa gatacaattg acgggttgaa agagttcgat aaatataaaa
      661 cacggatact ggattcgggg attaactgta aaaaacatcc gtggttatac ttccctcttg
      721 caaaatcctc accggggatg attgttatgg catgcaataa aaattataac caacatgaa
//
LOCUS       DAZFRJ010000555          777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000555 DAZFRJ010000000
VERSION     DAZFRJ010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.561"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>777
                     /gene="yjiC"
                     /locus_tag="ACHKUN_005281"
     CDS             <1..>777
                     /gene="yjiC"
                     /locus_tag="ACHKUN_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6663318.1"
                     /translation="LQSQIITDNHFLHHPKVDSELTRKYERARLDTENIYLLPLARGN
                     NHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKITSALDEIY
                     GGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYAGTTIGYIS
                     TLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDPIIGIEA
                     YPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFEL"
BASE COUNT          253 a          161 c          155 g          208 t
ORIGIN      
        1 ccttcagagc caaattatta cagataatca cttccttcat catccaaaag tcgacagtga
       61 gttaacacgt aaatatgaac gtgcacgcct ggatacggaa aatatctatc ttctgcctct
      121 tgctcgtggc aataaccata attacgatgg aaaaagcgtt gtcgaaatta gaaaacttga
      181 tattagtaaa gagtcctggc catttaatta tgtgacagaa gcatgcagag aatccgatgg
      241 tattacaaca acaggacgta tgctctatcg aaacttgaaa atcacctcag ctttagacga
      301 aatatatggt ggcatatgta aaaaggcgca tgctgctaca gaattagcag aaggtttacg
      361 gctaaattta tttatgaaga gccctttcga tcctgttgag aactatacag ttcatgaaat
      421 aacattaggc cctggttgca atgttccagg ttacgccgga accacgatag gatatatttc
      481 aacccttcca gtttctcagg caaaaagatg gaccaatgag cagccaagaa ttgatatcta
      541 tattgatcag ataataactg tctcaggggt agcaaattct tccggattcg cccttgccgc
      601 attacttaat gctaatattg agatgggaaa tgacccaatc attggtatag aagcctatcc
      661 tggtaccgcg gagatccatg caaaaatggg atataaagtt atccccggtg atgaagatgc
      721 gccactcaaa cgcatgactc tgcaaccgtc atctcttcca gaactgtttg aattaaa
//
LOCUS       DAZFRJ010000556          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000556 DAZFRJ010000000
VERSION     DAZFRJ010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.562"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     repeat_region   94..671
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=98..122
                     /rpt_unit_seq="tccccgcgccagcggggataaaccg"
BASE COUNT          174 a          214 c          204 g          174 t
ORIGIN      
        1 aacataatgg atgtgttgtt tgtgtgatac tataaagttg gtagattgtg actggcttaa
       61 aaaatcatta attaataata ggttatgttt agagtgttcc ccgcgccagc ggggataaac
      121 cgatctcctc atcctcccag ctaccgacag tagtgtgttc cccgcgccag cggggataaa
      181 ccgcaagatc tgccgcgagt ggtttcaccc ggcaggtgtt ccccgcgcca gcggggataa
      241 accggatatt tgggcgtaat accgcccgga tagtgtgtgt tccccgcgcc agcggggata
      301 aaccgtggta cggaacatgc tgtctatatt tcgaccagtg ttccccgcgc cagcggggat
      361 aaaccgccag tggagccgcc gcgaatttac acacgattgt gttccccgcg ccagcgggga
      421 taaaccgtaa ggccgtcgcc ggatcagcct ggctatgccg agctccccgc gccagcgggg
      481 ataaaccgtt cttgcgggtg ttgcaaatat tcttcacgta gagctccccg cgccagcggg
      541 gataaaccgg agcctgacga gactactgag gccgttctgt cgagttcccc gcgccagcgg
      601 ggataaaccg gacgccgccg ccgcgaagcc gtttccgatg ttgagctccc cgcgccagcg
      661 gggataaacc accatataac ccgttatctc tttctcaagt ttttatatta gcagtacttg
      721 taataagcaa catatccacg taacacctca tgttcaaaat agttct
//
LOCUS       DAZFRJ010000557          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000557 DAZFRJ010000000
VERSION     DAZFRJ010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.563"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>766
                     /locus_tag="ACHKUN_005282"
     CDS             <1..>766
                     /locus_tag="ACHKUN_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663319.1"
                     /translation="RFTLCLTSTLLTMSPLLMLNGAVKTGSIVRTIGRGVSYVDIPLA
                     LAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLSLMSSLSGAIMRSL
                     EKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNPDILNTQAIALCVD
                     LQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQGERAILNTSQSET
                     CKHKIDEIYEKRFSETELNTLPNEMKNFLIFLNKTHE"
BASE COUNT          292 a          151 c          121 g          202 t
ORIGIN      
        1 cgatttactc tatgtttaac aagtacatta ctaaccatgt caccactgtt aatgcttaat
       61 ggagcagtta aaacaggtag catagtaaga actataggta ggggagtgag ttatgttgat
      121 ataccattgg ccttagctat attaggtgac tcgtggtata aagcttataa acatggttct
      181 tctgataacc caaattctgc tcagagattt atatcgcaag aagcggcctt taaaaccacc
      241 cagcgggtat taacacaagg attaagtctg atgtcctctt tatcgggagc aatcatgcgc
      301 tctcttgaga aaggcacacc accacaaatg atgtctttat tcatcgtaaa catactaaat
      361 ctattatttc atcaaaatcc atatgaaggg gcatcagcaa gtgctaatgc tttgaaaaga
      421 tcaacttact cccataatcc ggacatacta aatactcagg caatagctct ttgtgttgac
      481 ctccagcata caaaaaatat aaccatgcca cttttcaaaa caaaagacag gatatcttac
      541 gcattcaatg gacaaagaac atccccagaa gaccaaaaac aaatactgaa agaagttata
      601 aactcctgta cccaaggaga aagagccatt ttaaatacat cacaatcaga aacatgcaaa
      661 cataaaatcg atgagattta tgaaaaaaga ttctcagaaa cagaactaaa tacattacca
      721 aacgaaatga aaaatttctt gatattttta aacaaaactc atgaaa
//
LOCUS       DAZFRJ010000558          765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000558 DAZFRJ010000000
VERSION     DAZFRJ010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.564"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(261..>765)
                     /locus_tag="ACHKUN_005283"
     CDS             complement(261..>765)
                     /locus_tag="ACHKUN_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO6663320.1"
                     /translation="LSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKARTQQR
                     HHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSFLSGG
                     VTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNAMISN
                     SFSNSVV"
BASE COUNT          247 a          153 c          134 g          231 t
ORIGIN      
        1 ggttatttat taaagaatga agtaattagt ttattgtcgt aagatgtttt atcctgataa
       61 attatgagct ggaaggaatt catccattca gctcaatccc ctcctcatca cagaatctct
      121 gacttctgcc agccaaactc actctgaata ttcatcccat attttcctaa aaacaatgta
      181 ttatcgacat aaaacaaaca gaaaaactcc agaatcacaa ttattctgga gttaaaccag
      241 gaggaaagct caaatgataa tcacactacg gagttagaga aagagtttga tatcatagca
      301 ttactataat caaggttttc agtagactgt acgcgacgta acaaagaaga taatcctctt
      361 tccgttaaga ccccctccag tggagttcga ggaaaccagg ttggagaaag tggagtgata
      421 tgcctctcat ttagtttact ttccaaagcc ttagtcacac cgccagaaag aaagctaaat
      481 aatcttgaga gacacccact ttcctgagtc atcttttgta ctttgtacac ataattatct
      541 gttttgtaat ctttaataaa acgaacagaa tattctttgc cccgaaagga gacaacggag
      601 taatccgcca tttctgaata tactcgatgg tggcgttgtt gtgttcgggc tttaagctga
      661 atttcatcta cgatatcttc attcaataca tgaagagttc tgctaaactt gccccatgac
      721 atatggtgac ttgtcagcga agtattgagt attattcctg ataac
//
LOCUS       DAZFRJ010000559          764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000559 DAZFRJ010000000
VERSION     DAZFRJ010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.565"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..666
                     /locus_tag="ACHKUN_005284"
     CDS             <1..666
                     /locus_tag="ACHKUN_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO6663321.1"
                     /translation="LISKEGTTEWCAEYDEWGNQLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLRGRYITQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPI
                     PWPKSPAQQQADDNAAKALTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADT
                     GITDRVNDIINDRFWSDGKKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSND
                     KKR"
     gene            687..>764
                     /locus_tag="ACHKUN_005285"
     CDS             687..>764
                     /locus_tag="ACHKUN_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO6663322.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDD"
BASE COUNT          258 a          149 c          184 g          173 t
ORIGIN      
        1 cttatcagca aggaagggac aacagaatgg tgcgcagaat acgatgaatg gggtaaccag
       61 ctgaatgaag agaacccgca ccagctgcag cagctcatcc gcctgccggg gcagcagtat
      121 gatgaggagt ccggcctgta ttataaccgc caccgctatt atgacccgct gcgggggagg
      181 tatatcactc aggatccgat tgggctgaag gggggatgga atttttatca gtatccgttg
      241 aatccagtta cgaatacaga tcctctgggg ttagaagttt ttcctagacc attccccttg
      301 ccaattccat ggcccaaaag ccctgcacag cagcaagcag atgataatgc tgcaaaagca
      361 ttgacaaaat ggtggaacga tacagcatca caaagaatat ttgactctct aatattgaat
      421 aatccgggac tagcattaga tataacaatg atagcttctc gtggaaatgt tgcagacaca
      481 gggataactg atcgtgtcaa tgacataata aatgacagat tctggagtga tgggaaaaaa
      541 accgacagat gtgacgtact tcaggaacta attgattgtg gtgatattag tgctaaagat
      601 gcaaaaagca cacagaaagc ctggaattgt cgtcactcca gacagtcaaa cgataaaaaa
      661 agatagccct tgtggaggtt cctgcaatgt caaatacata ccagaaaaga aaggcaagta
      721 aagagtatgg tttatataat caatgtaaga aactaaatga tgat
//
LOCUS       DAZFRJ010000560          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000560 DAZFRJ010000000
VERSION     DAZFRJ010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.566"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..495
                     /locus_tag="ACHKUN_005286"
     CDS             61..495
                     /locus_tag="ACHKUN_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO6663323.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          169 a          168 c          235 g          184 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagt
//
LOCUS       DAZFRJ010000561          755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000561 DAZFRJ010000000
VERSION     DAZFRJ010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.567"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>755
                     /locus_tag="ACHKUN_005287"
     CDS             <1..>755
                     /locus_tag="ACHKUN_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6663324.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAR"
BASE COUNT          177 a          204 c          231 g          143 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaagccgaa gcatgaggtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcatgcag aacatgcgtg acgaagagct ggccattgcc
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagcctttg atccggttga ggtggatatg ggccgcagtg cggcaaacaa catcacacag
      301 tccggtggca cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggcgtg gtgaatatca tcgtgtttga tccgaaagga
      421 tgggcgctgt tccgttcctt caaagccgtc agggataagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcaaag cggtgtctta taagggaatg
      541 tatggcgatg tggccatcgt cgtgtattcc ggacagtacg tggaaaacgg cgtcaaaaag
      601 aacttcctgc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgtacc
      661 tatggctgta ttcaggatgc ggacgcacag cgcgaaggca ttaacgcctc tgcccgttac
      721 ccgaaaaact gggtgactac cggcgatccg gcgcg
//
LOCUS       DAZFRJ010000562          744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000562 DAZFRJ010000000
VERSION     DAZFRJ010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.568"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(358..>744)
                     /locus_tag="ACHKUN_005288"
     CDS             complement(358..>744)
                     /locus_tag="ACHKUN_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO6663325.1"
                     /translation="LDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCKYSS
                     SDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTCTAV
                     LTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          272 a          169 c           91 g          212 t
ORIGIN      
        1 attatcacat tcaaaaaaaa tactgacaac aaagatactc ttcagacaaa gttgtcatat
       61 gtaaattttc ccttaaaacg ttgctcaaaa aaaatataaa aatcatatgc cctaatcaac
      121 atcctcaagc actacgatat atgcagtatc ctattcatat attaccacca tatcagacaa
      181 atcgaatcac aacgagttct atcgtactct ttcatcttcg acactgtaaa ttttaagaac
      241 attcggctca tgccactaaa ccaagacata ataacaacgg agcaataaat gttcccactg
      301 aacatgtgca aacctaaaaa gacagtgtta caataatatt catccatggt ctctaaatca
      361 acgccacgca acaggataat acaatgaaaa tatttcagga aaagattctt ttgcatgctc
      421 cgttagcata tcagtcagca cagcggtaca agtaaaaaca ttatttcttc cttgtagcct
      481 gtctaaaata tcattgtaat aataaactga gccaaatacc tgaggatctg ttttataagc
      541 ttgttcaatc aatccatttg catatctccg tagaatctca ggagaatcat actctgtacc
      601 aaacatatca ctcgatgagt atttacagaa aaccccccct aaacaaaata atatttcagc
      661 ctgttttctt agtggcatat ctttcaatac atggctcccc ataatcatat ctacatgctg
      721 agcgttcagt tcccaaccat caag
//
LOCUS       DAZFRJ010000563          742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000563 DAZFRJ010000000
VERSION     DAZFRJ010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.569"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..645
                     /gene="sodC"
                     /locus_tag="ACHKUN_005289"
     CDS             118..645
                     /gene="sodC"
                     /locus_tag="ACHKUN_005289"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6663326.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          195 a          194 c          181 g          172 t
ORIGIN      
        1 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
       61 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      121 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      181 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      241 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      301 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      361 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      421 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      481 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      541 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      601 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      661 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      721 catcctccct ctctgagtta at
//
LOCUS       DAZFRJ010000564          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000564 DAZFRJ010000000
VERSION     DAZFRJ010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.570"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..429
                     /locus_tag="ACHKUN_005290"
     CDS             <1..429
                     /locus_tag="ACHKUN_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO6663327.1"
                     /translation="AAQNYQSTLAWYQATPDSPNAERDCDAALAAFKRHIRHREADII
                     AGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYDLRYGHPINADE
                     RHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            407..>741
                     /locus_tag="ACHKUN_005291"
     CDS             407..>741
                     /locus_tag="ACHKUN_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663328.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRA"
BASE COUNT          216 a          153 c          206 g          166 t
ORIGIN      
        1 gcagcacaaa actatcagtc gacgctggcg tggtatcagg ctaccccgga cagcccaaat
       61 gctgaacggg attgtgatgc ggctcttgct gcgtttaagc gtcatatccg tcatcgggaa
      121 gcggatatta tcgctggttt gctggatgga ctggaagaag caaaatcaca actcaacgag
      181 cagcgtgagt attacgaagg cgttatctct gatgggagca agcgtattgc tgaactggat
      241 gcgcgggaag ttcaattacc gactcgctac gaccttcgat atggacaccc gataaatgca
      301 gatgagcgac atgtcatgat acctaaagaa aatggcagtt ggctttacct gattgaccta
      361 gaacacgcat tacgcgtcgc tggcattcgc atcaaaggag aggagcatgg aaataaaacc
      421 agaggatgag ttaagcaata tcgttttatt tccggtaaaa gaggatgacc ctcgtaatca
      481 ggttaatttt ctttatgagc catcggaaag accatattgt catcacgcct ctgttcgggt
      541 tgacgaaaaa gagcgccagg tccgctgtaa aatctgcggt gcggttgtgg agccgtttga
      601 ctggatgctc tctgtggcga aaagagaaac cagactggca gatgatgtaa ggctattgcg
      661 ccaggaggaa caggaaaggc ggaaaaatat agaaaagtta attcagattg aacgtaacgc
      721 gaaagcgcgg atacgcaggg c
//
LOCUS       DAZFRJ010000565          740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000565 DAZFRJ010000000
VERSION     DAZFRJ010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.571"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>740
                     /locus_tag="ACHKUN_005292"
     CDS             <1..>740
                     /locus_tag="ACHKUN_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6663329.1"
                     /translation="NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREE
                     FIRLIDACRNQQAKNLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTV
                     PKTQAGTNRVIHLIKPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPE
                     VSARVKNYGDHFTVDSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIAN
                     QMGHADAQMVFQVYGKWMSENNNAQVAL"
BASE COUNT          229 a          149 c          177 g          185 t
ORIGIN      
        1 taattacatg acaaatctta acgccgtgtt ccagtttggt gttgataacg gttacctggc
       61 agataatccg tttaagggga tctcgccatt aaaggaatca agaaccattc cggatcctct
      121 ttcgcgggag gaatttatcc gtcttatcga tgcgtgcaga aatcagcaag caaaaaattt
      181 atggtgtgtt tctgtttata ctggcgttcg ccctggtgag ctgtgtgcac ttggatggga
      241 ggacatagat ctgaaaaatg gaacaatgat gatcaggaga aatttagcaa aagaccgttt
      301 cacggtacca aaaacacagg cgggaaccaa tcgggtcatt catcttatta agccagcaat
      361 cgacgctctc cggagtcaga tgacattaac gagactgagc aaagagcata tcattgatgt
      421 tcacctcaga gagtatggca gaacagaaaa acaaaaatgc acctttgttt ttcaacctga
      481 agtgtcagcg agagtaaaaa attatggtga ccattttacc gttgactcaa taaggcagat
      541 gtgggacgca gcgataaaac gtgccggact ccgccatcga aaatcatatc agtcgagaca
      601 tacttatgcc tgctggtcgc tgacagctgg tgctaacccg gcatttatag caaaccagat
      661 gggccatgca gatgcgcaaa tggtatttca ggtatacgga aaatggatgt ctgaaaacaa
      721 taatgcacag gtagctttgt
//
LOCUS       DAZFRJ010000566          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000566 DAZFRJ010000000
VERSION     DAZFRJ010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.572"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>732
                     /locus_tag="ACHKUN_005293"
     CDS             <1..>732
                     /locus_tag="ACHKUN_005293"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6663330.1"
                     /translation="FPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFYLIL
                     NTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQSLT
                     SLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQLAV
                     MGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEW
                     CKFGLTSEFINGDRGSNYPNKNEYV"
BASE COUNT          242 a          154 c          160 g          176 t
ORIGIN      
        1 ttttccctaa aattggcgga gcaattgcaa ctaataaacg aagaatttta gttcaggata
       61 cagctattga taacaactgt cttggaataa aaccatgtga tgcaatatcg ggggagtggt
      121 tctatctaat ccttaatact ctcgatatgt cgaagtatca atcaggaacc tcaatacccg
      181 cgataaatca aagtgtcata ggtagtatac caattgcgct tccttcttta aaaatgcaag
      241 agaagattgt ttcttatgtc ataaccctaa tgtccctttg cgaccaactg gaacagcaat
      301 ccctgaccag tctggacgca catcagcaac tggttgaaac cctgttggga acacttacag
      361 acagccaaaa cgccgaggaa ctggctgaaa actgggcgcg tattagcgag catttcgaca
      421 ccctatttac caccgaagcc agcgtggatg cgttaaaaca gaccattctg caactggccg
      481 taatgggcaa acttgtgccg caggatccga atgacgaacc agcctctgaa ctgctcaaac
      541 gaattgcgca ggaaaaagct caactggtga aagaaggaaa aataaaaaaa caaaaaccgt
      601 tgccgccaat tagcgatgag gaaaaaccgt ttgaacttcc ggaagggtgg gagtggtgta
      661 aatttggttt aacatcagag tttataaatg gcgatcgtgg aagtaattac cccaataaaa
      721 atgagtatgt tt
//
LOCUS       DAZFRJ010000567          730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000567 DAZFRJ010000000
VERSION     DAZFRJ010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.573"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..730
                     /locus_tag="ACHKUN_005294"
                     /pseudo
     CDS             3..730
                     /locus_tag="ACHKUN_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          193 a          202 c          197 g          138 t
ORIGIN      
        1 acatgacaaa aacagtatca accagtaaaa aaccccgtaa acagcattcg cctgaatttc
       61 gcagtgaagc cctgaagctt gctgaacgca tcggtgttac tgccgcagcc cgtgaactca
      121 gcctgtatga atcacagctc tacaactggc gcagtaaaca gcaaaatcag cagacgtctt
      181 ctgaacgtga actggagatg tctaccgaga ttgcacgtct caaacgccag ctggcagaac
      241 gggatgaaga gctggctatc ctccaaaagg ccgcgacata cttcgcgaag cgcctgaaat
      301 gaagtatgtc tttattgaaa aacatcaggc tgagttcagc atcaaagcaa tgtgccgcgt
      361 gctccgggtg gcccgcagcg gctggtatac gtggtgtcag cggcggacaa ggataagcac
      421 gcgtcagcag ttccgccaac actgcgacag cgttgtcctc gcggctttta cccggtcaaa
      481 acagcgttac ggtgccccac gcctgacgga tgaactgcgt gctcagggtt acccctttaa
      541 cgtaaaaacc gtggcggcaa gcctgcgccg tcagggactg agggcaaagg cctcccggaa
      601 gttcagcccg gtcagctacc gcgcacacgg cctgcctgtg tcagaaaatc tgttggagca
      661 ggatttttac gccagtggcc cgaaccagaa gtgggcagga gacatcacgt acttacgtac
      721 agatgaaggc
//
LOCUS       DAZFRJ010000568          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000568 DAZFRJ010000000
VERSION     DAZFRJ010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.574"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..183
                     /locus_tag="ACHKUN_005295"
     CDS             <1..183
                     /locus_tag="ACHKUN_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO6663331.1"
                     /translation="MRNSRGAEICSLYDKDALVQLVETGGAHPLSREPITESMIMRKD
                     ECHFDSKKEAFVASDA"
     gene            complement(311..>715)
                     /gene="espW"
                     /locus_tag="ACHKUN_005296"
     CDS             complement(311..>715)
                     /gene="espW"
                     /locus_tag="ACHKUN_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO6663332.1"
                     /translation="ELGEYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRLYS
                     DEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLR
                     KSGAEEKDILACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          203 a          109 c          146 g          257 t
ORIGIN      
        1 atgagaaact cacgaggtgc tgagatatgc tctctatatg ataaggacgc gttagtgcaa
       61 cttgttgaaa ctggtggagc tcatcctctg agtcgagaac ctataacaga atcaatgatc
      121 atgagaaaag acgaatgtca ctttgattca aaaaaagaag cctttgttgc aagtgatgct
      181 taattttttc tgttggtgtg tttttatatt aatagtttat tataatagtg ccatgtaagg
      241 atatattgcc tgaacaatta ttcaggcaat attttccttg ctttatatga aatatataat
      301 atttggatcc ttaatttcta accaaggggt cccatgtttt tatgttatga tgcagcccat
      361 aatttcgggg gctacatgca agaatatctt tttcttcggc gcctgatttg cgtaaaaacg
      421 tggctgcgcc agaagaacaa ttaccttgtg tttctgtgaa tggcggtaac attttgtaag
      481 tcggtatgtt gttgagcatt gtttttatat tgtcagctgg aattgatttt tcttcatgta
      541 gtggtgtcgg gataagtgtt ccatttctct gaagtacttc atctgaatat aatcgatgta
      601 gcaacaaact ctgttttgtc tctattaatg agattgagtt gatattgggt aagtaacgaa
      661 ttgataactg actgagtttt attacatttc ctgaataaag atactcacct aactc
//
LOCUS       DAZFRJ010000569          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000569 DAZFRJ010000000
VERSION     DAZFRJ010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.575"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..640
                     /locus_tag="ACHKUN_005297"
     CDS             <1..640
                     /locus_tag="ACHKUN_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663333.1"
                     /translation="KDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAP
                     ETIMQEEMRKYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAA
                     KGHQKAIIVFQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEW
                     ATWSPVNYEYKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          224 a          149 c          159 g          177 t
ORIGIN      
        1 caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata ttcaagctgc
       61 gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg ttcaatccgg
      121 caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata ctgtttccac
      181 attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa
      241 tgagaacgag gatgttgcca gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt
      301 ggctgcaaaa gggcatcaga aagcgattat tgttttccag gatacgttgc agacaggaaa
      361 atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa aactcaccga
      421 cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa caggtgagtg
      481 ggctacctgg tcgccggtga attatgaata taaagtgctt ccaccattgc ccgacaagaa
      541 tgaagccagt acgactgata tgacagagca gcaaatcatg caacttaagc aaaagaccta
      601 taaagcgatg gtaaaagatt tggttaaccg ctatcaataa taaattatat ctgccgccag
      661 gaatttctgg cggcaataat acaaaattct ttgcataaga aatttttct
//
LOCUS       DAZFRJ010000570          708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000570 DAZFRJ010000000
VERSION     DAZFRJ010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.576"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..410)
                     /locus_tag="ACHKUN_005298"
     CDS             complement(<1..410)
                     /locus_tag="ACHKUN_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6663334.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(437..>708)
                     /locus_tag="ACHKUN_005299"
     CDS             complement(437..>708)
                     /locus_tag="ACHKUN_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6663335.1"
                     /translation="YLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPAD
                     ALNQATNRVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
BASE COUNT          155 a          222 c          170 g          161 t
ORIGIN      
        1 ccgctggctg ggacatatct cacacctccg gaaatccccg ctgaagccat cattaatgca
       61 acatcatcct cgctgacatc caccacatcc gcagaaggtg aaatatcgcg ccctccgtcc
      121 cctccgaacc ggacgcctcc ggcaactcct gtcgctttct gcatcagcat ttcttcctcg
      181 tccggcatct ccgtctgctc ttcctcacac gctggagcaa gcagactgaa atccgccgga
      241 tgcatatccg gatcgccaaa aaacaggctg agtacggcgt acatcagccc ggaaaaatga
      301 gcgtccagtt gggtatcctg aaaataatgc gtgcagtaaa aacgtcgcca gtcggcatat
      361 tcggtggatg tcatcccggc aagcatggcg cgccagtcgg gtctccccat ctctcgcgcc
      421 agtttcagga caaagttcag ctcgccttcg aatgcttttt tgatgttacc ggctcagtcg
      481 cttctgcttt cccggtttgt tcaggatcgg catcgtgccg gttatccagc atacctgaaa
      541 gataaagcac ccggttcgtt gcctgattca gtgcatcagc aggccatccc agcatcactt
      601 cacggcggat ctgctgcatc tctgtctccg gagaggccag agtgcctttg agggaatggg
      661 aatgccatag cgacatcgcc acaagcaggg atgccgtttc cagatatc
//
LOCUS       DAZFRJ010000571          705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000571 DAZFRJ010000000
VERSION     DAZFRJ010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.577"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..610
                     /locus_tag="ACHKUN_005300"
     CDS             <1..610
                     /locus_tag="ACHKUN_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO6663336.1"
                     /translation="FSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQS
                     NFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVIG
                     KETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFNT
                     QTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          226 a          137 c          167 g          175 t
ORIGIN      
        1 gtttagtcca atgacaatgg taggtagatc gttagctcag gcggctacac aaactcttag
       61 acctgcggtc accagagctg cgacacaagc gggggtggga gccagtggca tgaagttcgt
      121 gcccatgcag tcgaacttta tgattaatca tggcaaactg actaaccagc tacttcaggc
      181 cgtggccaaa caaacccgta gcggtgatac tcagcaatgg ttcaaacagg agcaaattac
      241 ttatatatcc agaacagtaa acagagctat tgatgactat tgcatgagta ataattcggt
      301 aattggtaag gaaaccaaaa gccacatttt caaggctgtt gaaagcgcga taaagcagcc
      361 tctcgatatg aattgtgcac aaacgtcgat tggccatttc ttacagtcca acaaatactt
      421 taataaaaaa gttgatgagc agtgtggcaa aggggtggat ccaattacac gctttaacac
      481 ccagaccagg atgatagaac aagtctcaca agaaatcttt gaacagaaat ttggtggttt
      541 gaaagttagt gatatcaaag ctctgactca gagcacgatt gcacagcatg tacaggatac
      601 tcggttgtag gaataacctg ttatatatgc gtgttgggca taagttaagc ttcctggtgt
      661 atagcgtagt ctttgctgat gtaagaaaag gctatatacg gaaac
//
LOCUS       DAZFRJ010000572          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000572 DAZFRJ010000000
VERSION     DAZFRJ010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.578"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            95..>702
                     /gene="nleG"
                     /locus_tag="ACHKUN_005301"
     CDS             95..>702
                     /gene="nleG"
                     /locus_tag="ACHKUN_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO6663337.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQY"
BASE COUNT          214 a          146 c          136 g          206 t
ORIGIN      
        1 aatttaattt tttgtttgaa tcctatcaca tatttagtat cttgactcct gtcaccaata
       61 tggttcaata acatagacta aagagggaga aaatatgcct gtcatattaa acttttcgag
      121 tgaaagagta ttgtctgaaa gtgaactgga ggctttacgt catgtcggaa gagttagtca
      181 gagtgaacag cttgttgtga gaggccgcac tatgagactt catcatattt catttatgga
      241 cagttttagc gtggaaccgg tttccggcgg actgttggat cgccttagtg caagagggca
      301 tcgccttctc gcagaaaatc tggagataca gcttaacaga ggccatactt ttttgcaggc
      361 attccgttta tatatggaac agagccgtgc aaccccatgt acgcgccaaa acgtcagtag
      421 tgcaatacaa aacaaaatta actctcatgc atttactgtt agtcatcaag acttttcctg
      481 tcatgaacaa cacctgaatt gcccgataac attatgcatt cctgaaactg gtgtttttgt
      541 cagaaatgca aaaaactcag aaatatgttc attatatgac cacaatgcac tcactgaact
      601 tatccgacgc aacgctccac accctctcag ccgtgaaccc tttgttccgg aaatgattgt
      661 cagcaaagat gaatgccatt ttaatctaat agaacaatat tt
//
LOCUS       DAZFRJ010000573          701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000573 DAZFRJ010000000
VERSION     DAZFRJ010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.579"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>701
                     /locus_tag="ACHKUN_005302"
     CDS             <1..>701
                     /locus_tag="ACHKUN_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6663338.1"
                     /translation="LISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHS
                     MGGTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTK
                     EAAGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLE
                     TCKMKCSGNNIGRFI"
BASE COUNT          209 a          131 c          190 g          171 t
ORIGIN      
        1 ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat atgatgaatg gggcaacctg
       61 ctgaatgaag agaacccgca tcagctgcag cagcttatcc ggctgccggg gcagcagtat
      121 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcgg
      181 tatatcactc aggatccgat tgggctgaag gggggatgga atttttatca gtatccgttg
      241 aatccgatct caaatataga tccattagga ttagaaacac taaaatgcat taagccactg
      301 cattcaatgg gcggaactgg tgaaagaagc ggtccagata tatgggggaa tccgttctat
      361 catcaatatc tttgtgtccc agatggtaaa ggggactata cttgtggtgg ccaagaccaa
      421 cggggagaat caaaaggaga tggtctatgg gggccaggta aagcaagtaa tgatacaaaa
      481 gaagctgctg gccgttgtga cctcgttgaa accgataata gttgtgtgga gaactgttta
      541 aaagggaagt ttaaagaggt aaggccgcgt tattctgtat tgcctgatat attcacacct
      601 ataaatttag ggctatttaa aaactgccaa gactggtcta atgattcttt agaaacatgt
      661 aagatgaagt gctccggaaa taacattgga cgttttatta g
//
LOCUS       DAZFRJ010000574          699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000574 DAZFRJ010000000
VERSION     DAZFRJ010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.580"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(312..>699)
                     /locus_tag="ACHKUN_005303"
     CDS             complement(312..>699)
                     /locus_tag="ACHKUN_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6663339.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          144 a          199 c          182 g          174 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctacctg ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZFRJ010000575          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000575 DAZFRJ010000000
VERSION     DAZFRJ010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.581"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(15..665)
                     /locus_tag="ACHKUN_005304"
     CDS             complement(15..665)
                     /locus_tag="ACHKUN_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO6663340.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
BASE COUNT          192 a          136 c          140 g          223 t
ORIGIN      
        1 ttgtgttttt atttttaaat actgttttgt tgaagggggt atatgggtaa tgcgtaaaaa
       61 cattgttttg ttatattaaa atgacacttg tctttgctta taatcatttc tggagcaaag
      121 gggtcacggc tgagggggtg gagagcgtta cgcaggataa gttcagtcaa tgcttcctga
      181 tcatataatg cacatatttc tgaatttcga gcatttctga caaaaacacc tgtctcagga
      241 atatgtagcg ttatcggaca attaagatgc tcctcagtac aggaaaagtc ttgtaaacta
      301 acgggaaatg cattcgaatt tattcgatct agtagtgttt ttatgacgct ttcctgcaca
      361 agcggggcag cttgcctctg ctccagatat aagctgtatg cctgcagaaa agtgttacct
      421 ccgttaagtt gtcttgccag gctgtctgcg agatgatgag actctcttgc cccaagatgg
      481 tcccaaagtc caccaggaat aggctctaca ctaaaaccat ccataaactg gatataatga
      541 agcctcatat tacggcctcc tatagtaata gtgtcattct gattgcttct tgcaatatgt
      601 cgtaaagcct ccagctcatt ttccggcaat acacttccat tagaaaaatt taatatgact
      661 ggcatattat tatccttttc aacccatttt g
//
LOCUS       DAZFRJ010000576          688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000576 DAZFRJ010000000
VERSION     DAZFRJ010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.582"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..93)
                     /locus_tag="ACHKUN_005305"
     CDS             complement(<1..93)
                     /locus_tag="ACHKUN_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663341.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNS"
     gene            complement(77..>688)
                     /locus_tag="ACHKUN_005306"
     CDS             complement(77..>688)
                     /locus_tag="ACHKUN_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6663342.1"
                     /translation="QQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLN
                     PIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNG
                     AADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACG
                     DFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          197 a          171 c          116 g          204 t
ORIGIN      
        1 agaatttgaa ttgttatcaa acccaaggta aacaacaaat aaacaaaggg taattaaaaa
       61 aatgacaagt ttgcctctaa taatttgaag catctgaact cccttgcttt gcttttattg
      121 gattatctgc ttttatacca tctaacccga agagttgccc agtgttatat ttttcaatac
      181 atgcatcgct acatttggca gaaaaatcgc cacaagcaat accgacagtg ttatttttaa
      241 gatccatgat tctttcttta gctggctgac cttgtctatc ccctgccgcc tcatgatttt
      301 tacctatcac gtcagctatt gttgatccaa atgtcttagt cattcgacac atcaagtaac
      361 aatgtctcat tgcatcggcg gccccattat gagctccgcc aacaatagca tccatatcat
      421 aaccgaactg gtaagcactg ttagtaatgc tgtttgcagc aatcaatcca ggtacaccag
      481 atacggcata aattagtcca cctgatgtca tattctcaat cgcatctaac cccattgggt
      541 ctatcacttg tatgggattc aacggatact gatacatatt ccatccacct ctcaacccaa
      601 tcgggtccgg ggtgatatac cgcccctgca acggatcgta gtaccggtga cggttatagt
      661 acagccccga ctccttatca tactgctg
//
LOCUS       DAZFRJ010000577          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000577 DAZFRJ010000000
VERSION     DAZFRJ010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.583"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..466)
                     /locus_tag="ACHKUN_005307"
     CDS             complement(<1..466)
                     /locus_tag="ACHKUN_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO6663343.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRTLAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAG"
BASE COUNT          206 a          167 c          118 g          195 t
ORIGIN      
        1 ctccagcatt tctcataatc acaccatgct cagggatgca taatgtaatc gggcatgtta
       61 atgcctcttc agtcgccctc agagatgacg agttaacatt aaatgagcat ttttctattt
      121 gctctaacac caactctgtt tgcattacag aaggacgagg tggagaagca tacgggctgg
      181 gtatacttgc taaaatctgg cggccggata gccctccatt aaggtcgcgt tcaagacgat
      241 taacattatt caatgcagtt cgtaaagctc tctctgataa tccttcaggg cttacacttc
      301 ccataaagaa gcctaaaaag acagaacgta ttgtaacaat agtgctcccc atctgaacct
      361 gagctcctcc gttaaaagcc agtgttcgta tgccagaaag agtatcctgc ggtatttccc
      421 ccagtccccc actcgctgtt agatatggtg ttaaatttat tggcataata aaacccccat
      481 taacttcatt aacattttac ttgatttcct gttgaagcat cttacttatc acaatgcttt
      541 cacacaccat tatcagtctt gcaaaagata agactccatt atcaagccaa aataaattta
      601 acttcattaa caaacatcac catgacatga caacaaaaaa ccggagccag actccggttt
      661 tttgaagctg tcgggttact tcatcc
//
LOCUS       DAZFRJ010000578          685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000578 DAZFRJ010000000
VERSION     DAZFRJ010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.584"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..147)
                     /locus_tag="ACHKUN_005308"
                     /pseudo
     CDS             complement(64..147)
                     /locus_tag="ACHKUN_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            232..303
                     /locus_tag="ACHKUN_005309"
                     /pseudo
     CDS             232..303
                     /locus_tag="ACHKUN_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(314..556)
                     /locus_tag="ACHKUN_005310"
     CDS             complement(314..556)
                     /locus_tag="ACHKUN_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6663344.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
BASE COUNT          201 a          131 c          171 g          182 t
ORIGIN      
        1 atgatgggca atcgtgcaac attgtattgt atgtaaaggc agagctccga attttactgt
       61 tgtaagtggg cctggcggga atggtatcgc cagcatcatt ggcttgggtc cagtaaaaag
      121 agacgcagaa agatgcacaa taggcactac gcgtcatgca tggattaggt cgctcatcat
      181 ttacacagta ctgctaaaaa attattaagg aaagcgcggc acttcgtatg taagagcgtg
      241 tcgcggttgg ctggtgaact ttcgatagtg cgagtattga atgatttcca gccgttaccg
      301 atttacgtgt ttattagcga acaaaccact cgtcagcaga ctcccaggta tctttcagcg
      361 tctcctgaac aaaagttttt gcagaatctt tatcggcggt gcgagtaact gaaaggccat
      421 cgttgctggt ggatttgatg atcacttcta catcgtcata acgtttactg acacgtcgca
      481 gcatttcttg ttgcagggca ggaacagaac cttttggcat tttgttgatt ttggctgcgg
      541 ggtatctgtg gggcatatat gggacataaa aaggcctcaa ataaacagca aaatcgagat
      601 tagggatttt tagaaaaaat gcaactatct gaaaaaacgt agaaaacgcc aaggaaccca
      661 caggatgtga aaaaacacct gtgaa
//
LOCUS       DAZFRJ010000579          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000579 DAZFRJ010000000
VERSION     DAZFRJ010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.585"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>683
                     /locus_tag="ACHKUN_005311"
     CDS             <1..>683
                     /locus_tag="ACHKUN_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO6663345.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMA
                     DDGQLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTK
                     TLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIAT"
BASE COUNT          139 a          155 c          238 g          151 t
ORIGIN      
        1 cagcggacag gtaccgcttt ttcggataat gcggccctgt ttgcccgttc ggctgcctcg
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggct gaggcgggtt cggtgatcac ccagttcagc
      181 caggcgctgg cacagggtgt gttgcgcggt gaggaattta attcggtcaa tgaaagtggt
      241 gaccggatca ttcgtgcact ggctgcaggc atgggcgtgg cccgtaaaga tctgaaggcg
      301 atggcggacg atggtcaact gacggcggat aaagtcgttc ctgcgttaat cagccagctg
      361 ggggtattgc gtgatgaata tgccgccatg ccagaaacgg tttccggtag tatcacgaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggagtgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcgtgctgaa cggtgttgcc ggacagattg ataatgtggc aacagctgtg
      541 ggtgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cac
//
LOCUS       DAZFRJ010000580          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000580 DAZFRJ010000000
VERSION     DAZFRJ010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.586"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>683
                     /locus_tag="ACHKUN_005312"
     CDS             <1..>683
                     /locus_tag="ACHKUN_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO6663346.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMA
                     DDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASGVTK
                     TLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAVSATAGLVTAARNEVALAE
                     AQFRGTQIAT"
BASE COUNT          149 a          155 c          239 g          140 t
ORIGIN      
        1 cagcggacgg gcacggcatt ttcagataat gcggccctgt ttgcccgctc ggcagcctca
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttcgaca
      121 gggctgaaaa tctccggtgc cagtacggca gaggcgggct cggtgatcac ccagttcagt
      181 caggcgctgg cgcagggtgt attgcgtggt gaggagttta attcggtcaa tgaaagtggt
      241 gaccggatcg tacgtgcact ggctgcgggt atgggcgtgg cccgtaaaga tctgaaggca
      301 atggcggatg acggaaaact gacagcggat aaagtggtcc ccgcgttaat cagccagctg
      361 gggatattac gtgatgaata tgcggccatg ccggaaacgg tttccagtag tatcacaaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggtgcgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcatgctgaa cggtgttgcc ggacagattg ataatgtggc aacagccgtg
      541 ggcgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcggtg
      601 tctgccacgg caggacttgt gacggcagca cgtaatgaag tggcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cac
//
LOCUS       DAZFRJ010000581          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000581 DAZFRJ010000000
VERSION     DAZFRJ010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.587"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..75)
                     /gene="ariR"
                     /locus_tag="ACHKUN_005313"
                     /gene_synonym="ymgB"
     CDS             complement(<1..75)
                     /gene="ariR"
                     /locus_tag="ACHKUN_005313"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO6663347.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGN"
     gene            complement(104..376)
                     /gene="ymgA"
                     /locus_tag="ACHKUN_005314"
     CDS             complement(104..376)
                     /gene="ymgA"
                     /locus_tag="ACHKUN_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO6663348.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(419..655)
                     /gene="ycgZ"
                     /locus_tag="ACHKUN_005315"
     CDS             complement(419..655)
                     /gene="ycgZ"
                     /locus_tag="ACHKUN_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO6663349.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          186 a          147 c          112 g          230 t
ORIGIN      
        1 attacccgaa ctgcgaaagt aacttgctaa cgctttatca gttattgcat tatgaattgt
       61 agtatcttca agcatggtaa cctctcatct tacttatgaa attttaatgt attctgttta
      121 ttttcttacc attgaacata cttttattgt cagctgtcgc tgttcttcgg tttggccctc
      181 ctgaatcact attgctaaaa tcacgaatta tgctgtcaat taaatcgcgt cggaaagcat
      241 cttcctcagc cgatttcatt acgagtaatt gcttaataag agtctgcaat gaaaaattta
      301 tcttcatgcg cagtatctgg atcaccgcct ggccagtaat ttcatatttt atacgttcat
      361 tatcagatgt cttcataacg acttcatagc taacctgtta aataatcagg ctgttgcatt
      421 attcaaaaag caacccaatt agtgcattat aatgtttctg ttcctcttcc ccagtcgcgt
      481 gttccagtcg gcaaagaagt ttggcacaaa gcgactttcg actcagatta cgtccatctt
      541 tcaaaatctc agtcacaatt tccccgagag tttcctgctg agtaggcaag ttagcttttg
      601 caaagtaacg agtgattgct cccgcagaat ctaaagtcac tgaattttga tgcatgctac
      661 gcctctgtta aaaat
//
LOCUS       DAZFRJ010000582          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000582 DAZFRJ010000000
VERSION     DAZFRJ010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.588"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..602)
                     /locus_tag="ACHKUN_005316"
     CDS             complement(<1..602)
                     /locus_tag="ACHKUN_005316"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6663350.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAA"
     gene            complement(583..>675)
                     /locus_tag="ACHKUN_005317"
     CDS             complement(583..>675)
                     /locus_tag="ACHKUN_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663351.1"
                     /translation="GLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          131 a          220 c          197 g          127 t
ORIGIN      
        1 gcagcaccgt aattactgtg agccatcatg acgccgatgg agcctgtccg ggcggtctgc
       61 gtgaccagac gccgggaggc ggcgctggca agcagctgac ctgcactgca gttcatgtcg
      121 ttggccagcg cccataccgg ttttatgtca cgcacacggg cgatgatgtc agcacagtca
      181 aatgctcccg ccaccattcc gcccggtgtg tccatatcga gcagaatgcc gtccaccatc
      241 gggtcgctgg cagcctgttg cagacgggcg ataatgccgt tgtaaccggt catccccgaa
      301 tacggctgca gcgcccgcgt ccggctgacc agcgtaccgg acaccggcag cacggcgatg
      361 ccgttcatga cctgataact ccgggcctgt cgtggtccgt catcatcacc ggataacgcc
      421 agcgccgcgg gtgcctctcc ggcagtcagg ctgtcgccgg atactgcatc cgtcaggcgg
      481 ctgatcccaa gctggcctgc aagcgcacaa aagaaaaccc gcgcataggc gggttcaagc
      541 atcagcggct cattaaaagc catgctggca atatgcggga gattacgcag ctctgctgtc
      601 actcttctcc tcctctgttg attgtcgcag cccggattca aatgccgcag ccgcccaggc
      661 gggcggttta agacc
//
LOCUS       DAZFRJ010000583          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000583 DAZFRJ010000000
VERSION     DAZFRJ010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.589"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..602)
                     /locus_tag="ACHKUN_005318"
     CDS             complement(<1..602)
                     /locus_tag="ACHKUN_005318"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6663352.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPTALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAA"
     gene            complement(583..>675)
                     /locus_tag="ACHKUN_005319"
     CDS             complement(583..>675)
                     /locus_tag="ACHKUN_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663353.1"
                     /translation="GLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          131 a          221 c          196 g          127 t
ORIGIN      
        1 gcagcaccgt aattactgtg agccatcatg acgccgatgg agcctgtccg ggcggtctgc
       61 gtgaccagac gccgggaggc ggcgctggca agcagctgac ctgcactgca gttcatgtcg
      121 ttggccagcg cccataccgg ttttatgtct cgcacacggg cgatgatgtc agcacagtca
      181 aatgctcccg ccaccatccc gcccggtgtg tccatatcca gcagaatgcc gtccaccatc
      241 ggatcgctgg cagcctgttg cagacgggcg ataatgccgt tgtaaccggt cattcccgaa
      301 tacggctgca gcgcccgcgt ccggctgacc agcgtaccgg acaccggcag cacggcgatg
      361 ccgttcatga cctgataact gcgggcctgt cgtggtccgt catcatcacc ggataacgcc
      421 agcgccgtgg gtgcctctcc ggcagtcagg ctgtcgccgg acaccgcatc cgtcaggcgg
      481 ctgatcccaa gctggcctgc aagcgcacaa aagaaaaccc gcgcataggc gggttcaagc
      541 atcagcggct cattaaaagc catgctggca atatgcggga gattacgcag ctctgctgtc
      601 actcttctcc tcctctgttg attgtcgcag cccggattca aatgccgcag ccgcccaggc
      661 gggcggttta agacc
//
LOCUS       DAZFRJ010000584          672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000584 DAZFRJ010000000
VERSION     DAZFRJ010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.590"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..629)
                     /locus_tag="ACHKUN_005320"
     CDS             complement(<1..629)
                     /locus_tag="ACHKUN_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6663354.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
BASE COUNT          215 a          132 c          152 g          173 t
ORIGIN      
        1 tacaatattt gtggccaggg attgttttcc gccaaatatc gcgttgcaga aagtcggcaa
       61 ataattaaca ctttgtctcc tttagtgaaa aattttcctt cttcaggctc cagtagtaaa
      121 taatgtattt ggccaaattg atcggtgagt tttccttcgc agggattacc tgaagtggct
      181 tgatgaccgg taattaatgc catgctacca atatattctt cttctgtaat agctgaacta
      241 tggtcgcgtg gaatccaggg cgcaactatt ttaccggtat aatgcaccgc aataatcgta
      301 aacagtaaac tgacaggaac cacgaacagg ttcgacagtg gtgattgcca gaccatgatg
      361 caggcatgct gcaacaggat gccaataaga ccaaaaaaac cagccagcaa gcagagaacg
      421 acgagagctg gcaatcttcc gatattgaga taatgaagtg cctggctaat atgacctgtg
      481 gttatggaat cataatggtc aagatgtgca tcaagagcac cggagagcat gtgaccgcag
      541 ataagcgcta aaatttctaa aaggccgatc aacagtacaa aggaaatagc gaaaagataa
      601 ggggtattat agtcggcgaa taaaatcata tgtcactcca atgtccattt agtgacgcaa
      661 tatttacttt gg
//
LOCUS       DAZFRJ010000585          670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000585 DAZFRJ010000000
VERSION     DAZFRJ010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.591"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            272..487
                     /gene="essD"
                     /locus_tag="ACHKUN_005321"
     CDS             272..487
                     /gene="essD"
                     /locus_tag="ACHKUN_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO6663355.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            487..>670
                     /gene="rrrD"
                     /locus_tag="ACHKUN_005322"
     CDS             487..>670
                     /gene="rrrD"
                     /locus_tag="ACHKUN_005322"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO6663356.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          166 a          138 c          165 g          201 t
ORIGIN      
        1 tgtagtggtg ttttcaatac tcggtagcat tctcgcaaat atcatttagt ggtttacgta
       61 cgtaaaaaat tggttatgct gttaagagtg gttacttcgt cacacagctt aaacccgccg
      121 tcgagcgggt ttttccattt tttgagtctc gatattagct gataacccaa tacctgagtt
      181 attcactgac tccgagtctg ttacgtttct gcttttttgc gatacgttgt attccctcaa
      241 tttacacccg ctttgtctgc gaggtggggt tatgaaatcc atggataagt taacaacggg
      301 tgtcgcctat ggcacctcag caggtagtgc cggttactgg tttttacagc tgctcgataa
      361 agtcacgccc tcacagtggg cagcaatagg tgtgctgggt agcctggtat ttggcctgct
      421 gacgtacctg acaaaccttt atttcaagat taaagaagat aagcgcaagg ctgcgagagg
      481 tgaataatgc ctccatcatt acgaaaagcc gttgctgctg ctattggtgg cggagcaatt
      541 gctatagcat cagtgttaat cactggccca agtggtaacg atggtctgga aggtgtcagc
      601 tacataccat acaaagatat tgttggtgta tggactgtat gtcacgggca tacaggaaaa
      661 gacatcatgc
//
LOCUS       DAZFRJ010000586          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000586 DAZFRJ010000000
VERSION     DAZFRJ010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.592"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUN_005323"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKUN_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO6663357.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHKUN_005324"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHKUN_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO6663358.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
BASE COUNT          164 a          154 c          163 g          186 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcg
//
LOCUS       DAZFRJ010000587          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000587 DAZFRJ010000000
VERSION     DAZFRJ010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.593"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>663
                     /locus_tag="ACHKUN_005325"
     CDS             <1..>663
                     /locus_tag="ACHKUN_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6663359.1"
                     /translation="FIVLTPAFLASIAFIVLPIVITVLVSFTNYAAPNHIPPKNLVDW
                     VGIKNFIMLFKFKIWSDTFLGVALWTFIWAICATIFTFSFGFILALALAKKNIRFAKV
                     WRLIFILPYAIPTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDFLSVPLNAKITVIAV
                     CVWVGAPYFMLLIAGALTNISRDLYEASEVDGATKWQQFKEITLPMVLHQVAPTLVMT
                     FA"
BASE COUNT          175 a          120 c          133 g          235 t
ORIGIN      
        1 catttatagt cctaacgccc gcatttttag cgagtatagc attcattgtt ttacctattg
       61 taattacagt tcttgtatcg tttacaaact atgctgcgcc aaatcacatc ccaccaaaga
      121 acctggttga ttgggttggg attaaaaatt tcattatgtt gtttaaattt aaaatatggt
      181 ctgatacgtt tctaggtgtc gctctctgga catttatttg ggcaatttgc gcgacgattt
      241 ttaccttcag ttttggcttt attctggctt tagcactggc taaaaaaaat atacgtttcg
      301 caaaagtctg gcgattgata tttatactgc catatgccat acccacattt gtaacgttac
      361 ttattttccg tcttctttta aatggaattg gtccggttaa ctcgtttctg aacagtatcg
      421 gaatgaattc agttgatttt ctgtcggttc cattaaatgc aaaaataaca gtaattgccg
      481 tttgtgtttg ggtaggggca ccgtatttta tgctattgat cgcgggtgca ttaacaaaca
      541 tatcccgaga tttgtatgag gctagtgagg tcgatggcgc aactaaatgg caacagttta
      601 aggaaattac attgccaatg gtgttacatc aggttgcgcc aactctggta atgacatttg
      661 cat
//
LOCUS       DAZFRJ010000588          662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000588 DAZFRJ010000000
VERSION     DAZFRJ010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.594"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..>662
                     /locus_tag="ACHKUN_005326"
     CDS             43..>662
                     /locus_tag="ACHKUN_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6663360.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          173 a          162 c          169 g          158 t
ORIGIN      
        1 cctacttttt tattgccttc aaataaattt aaggagttcg aaatggacca gaagctgtta
       61 acggatttcc gctcagaact actcgattca cgttttggcg caaaggccat ttctactatc
      121 gcggagtcaa aacgatttcc gctgcacgaa atgcgcgatg atgtcgcatt ccagattatc
      181 aatgatgaat tatatcttga tggcaacgct cgtcagaacc tggccacttt ctgccagacc
      241 tgggacgacg aaaacgtcca taaattgatg gatttgtcga tcaataaaaa ctggatcgac
      301 aaagaagaat atccgcaatc cgcagccatc gacctgcgtt gcgtaaatat ggttgccgat
      361 ctgtggcatg cgcctgcgcc gaaaaatggt caggccgttg gcaccaacac cattggttct
      421 tccgaggcct gtatgctcgg cgggatggcg atgaaatggc gttggcgcaa gcgtatggaa
      481 gctgcaggca aaccaacgga taaaccaaac ctggtgtgcg gtccggtaca aatctgctgg
      541 cataaattcg cccgctactg ggatgtggag ctgcgtgaga tccctatgcg ccccggtcag
      601 ttgtttatgg acccgaaacg catgattgaa gcctgtgacg aaaacaccat cggcgtggtg
      661 cc
//
LOCUS       DAZFRJ010000589          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000589 DAZFRJ010000000
VERSION     DAZFRJ010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.595"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..227)
                     /locus_tag="ACHKUN_005327"
     CDS             complement(<1..227)
                     /locus_tag="ACHKUN_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663361.1"
                     /translation="MLKSFCLNFDGMYLSIVSFLSEKFNLKRVGKMELESKEDYEETT
                     HHNVASSVVTATHDNLDGREFFELCSNPALV"
     gene            complement(205..>660)
                     /locus_tag="ACHKUN_005328"
     CDS             complement(205..>660)
                     /locus_tag="ACHKUN_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6663362.1"
                     /translation="ERVFNEGSRGFSPSQIIQKILRKNAKHPVRRNISSLFNAEQLAR
                     LKLTLPPCNNCKTPRLTDNQKFCHHCGNQLIDESAYNQCMSIPLSKVPHLTSWQMQKL
                     SGLEKVKTIGDVLSLQDPGSELRKIHRIGPKIANKIMDKVLLHVEEFLS"
BASE COUNT          195 a          125 c          110 g          230 t
ORIGIN      
        1 aaaactagag ctggattact acatagttca aaaaactctc ttccatctaa gttatcatgg
       61 gttgcagtga caactgagga cgccacatta tgatgagtgg tctcttcata atcctctttt
      121 gattctagtt ccattttccc gactcttttt aaattaaact tttcagataa gaaagacacg
      181 attgacaagt acatgccatc aaaattaaga caaaaactct tcaacatgta ataacacttt
      241 gtccataatt ttattggcaa ttttgggtcc tattctgtga atttttctta actcactccc
      301 agggtcttgt aaagacaata catctcctat agtttttact ttttccagcc cagatagttt
      361 ttgcatttgc caagatgtta gatgtggtac ttttgacagt ggaatagaca tgcactgatt
      421 ataggccgac tcatcaatca attgattccc acaatgatgg caaaattttt gattgtcagt
      481 caatcgcgga gtcttgcagt tattgcatgg aggtaaagtt agtttaagtc tcgcaagttg
      541 ctcagcattg aataatgatg aaatgtttct tctgacagga tgcttagcat tctttcttag
      601 tattttttga ataatctgag aaggagaaaa acctctactt ccttcgttaa acactctttc
//
LOCUS       DAZFRJ010000590          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000590 DAZFRJ010000000
VERSION     DAZFRJ010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.596"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..78)
                     /locus_tag="ACHKUN_005329"
     CDS             complement(<1..78)
                     /locus_tag="ACHKUN_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663363.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYI"
     gene            160..>653
                     /locus_tag="ACHKUN_005330"
     CDS             160..>653
                     /locus_tag="ACHKUN_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFO6663364.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSAILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKMFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLYL"
BASE COUNT          229 a           94 c          156 g          174 t
ORIGIN      
        1 aatataaaat acatacagaa agacatgaga gcaagggacg gggcagggca agatcatgaa
       61 aattgtgatg taaatcacga ttttcatctt ggctttaacg cctacaggtg atcaaaattt
      121 aacctttgga ataactaaaa agataaaaaa ggacgccagg tgagtattca aagtttgctt
      181 gattatattt cagtgatccc tgatatacga caacaaggaa aggttaaaca taaattatct
      241 gctattttgt ttctcaccgt atgtgcagta attgcaggtg ccgatgagtg gcaggaaatt
      301 gaagattttg gacatgaaag acttgaatgg ctaaagaaat atggtgattt tgataatggc
      361 attccggtcg atgacaccat tgcacgcgtt gtgagtaaca ttgacagttt ggcctttgaa
      421 aagatgttta ttgaatggat gcaggagtgc catgaaatca ctgatggtga aattatagca
      481 atagatggaa agaccataag aggctccttt gataagggaa aaagaaaagg agcaatccat
      541 atggtgagtg cattctcgaa cgaaaatggt gttgtactgg ggcaggtgaa aacggaagcc
      601 aaaagtaatg agattacagc cattccagag ttgcttaacc tactgtattt aaa
//
LOCUS       DAZFRJ010000591          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000591 DAZFRJ010000000
VERSION     DAZFRJ010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.597"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(25..339)
                     /locus_tag="ACHKUN_005331"
     CDS             complement(25..339)
                     /locus_tag="ACHKUN_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6663365.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          184 a          170 c           93 g          200 t
ORIGIN      
        1 ggcaataact ctctcagata ttttttagca tttttttgac cgcgcgtttc cggacgtatt
       61 ctgttctcct gtccctttat atcgtcggaa tacccgccgc tcttcaaatc ccattcccaa
      121 ctcagaatgt agtctgttga ccgcttgttt tatttcggtc aggttcaccg gtgaaaccgg
      181 agtccggcgc gccttacgca aacactctgc tcgtttctgt gccgccactt ttcttttctg
      241 gtcatcactt agctgtacca tcacttttgc ccatcgttca gctgctctcc ggtacagtcc
      301 ttttttctcc agacattctg ccacgtgatc atgtagcata agtgacctcc gattatctac
      361 agactgccat cctgaattta ccttccctta atgaaataac aataaaaaac aaaccacgca
      421 aaaacaataa aacaacacac aaaaaaaact aaataataaa caaaaataat caccttattt
      481 tattattttt tgagggagca attactgaac aaaaaacgct gactatatac tcaaaaccaa
      541 acaactattc tgccaatcag gtatcatggc aacacacgga attaccgtgt ttttgccttc
      601 tctgcccata caatacgggc atatacttca ttctctattg taatatt
//
LOCUS       DAZFRJ010000592          646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000592 DAZFRJ010000000
VERSION     DAZFRJ010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.598"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>646
                     /locus_tag="ACHKUN_005332"
     CDS             <1..>646
                     /locus_tag="ACHKUN_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6663366.1"
                     /translation="KRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRN
                     SRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTV
                     FGPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTT
                     TVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQ"
BASE COUNT          186 a          159 c          173 g          128 t
ORIGIN      
        1 gaagcgggct gcttttcccc gattcaaaaa gcggggacag aatgatgcat tccgctaccc
       61 gcagggcgta aagctcgatc aggaaaacag ccgtattttt ctgccgaaac tgggctggat
      121 gcgctaccgg aatagccgtc aggtcacggg tgttgtgaaa aatgtcacgg tcagccagtc
      181 ctgcggtaag tggtacatca gtattcagac agaaagtgaa gtatcaactc cggttcaccc
      241 ttcagcatca atgatcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg
      301 cacagtcttt gggcctgtaa acagttttca gaaaaaccag aagacgctgg cgagacttca
      361 gcgacagtta agccgcaagg tcaaattcag caataactgg cagaagcaga aacgcaaaat
      421 acagcgactg cattcccgta tcgcaaatat ccgcagggac taccttcata aagtcacaac
      481 gaccgtcagc aaaaaccacg caatgatagt cattgaggat ttgaaggtca gcaacatgtc
      541 aaagtcagca gcgggtacgg tcagccagcc ggggcgcaat gtccgggcaa aatcaggttt
      601 aaaccgttcg atactggatc agggctggta tgaaatgcgc cgccag
//
LOCUS       DAZFRJ010000593          645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000593 DAZFRJ010000000
VERSION     DAZFRJ010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.599"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..192)
                     /locus_tag="ACHKUN_005333"
     CDS             complement(<1..192)
                     /locus_tag="ACHKUN_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6663367.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSG"
     gene            complement(192..461)
                     /gene="escS"
                     /locus_tag="ACHKUN_005334"
     CDS             complement(192..461)
                     /gene="escS"
                     /locus_tag="ACHKUN_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO6663368.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(461..>645)
                     /gene="escR"
                     /locus_tag="ACHKUN_005335"
     CDS             complement(461..>645)
                     /gene="escR"
                     /locus_tag="ACHKUN_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO6663369.1"
                     /translation="KIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILLFI
                     LVGGWQKLFEFLLVVN"
BASE COUNT          237 a          121 c          112 g          175 t
ORIGIN      
        1 tccggatggt aatttttccg aaaatgtata gttctctact ataattggaa gtgtaaacgc
       61 aatcataatt gagttgcgta taaagttact gagcaaaaca cctgttgaaa atattggtag
      121 aataataaac atccccagcg gcctgagtat acaaaaaaat gatgatacta tgaccgtcat
      181 tatttcattc attagccatt caccttcgga atcatttcaa atattatcga cgagaagttg
      241 atgattgttg ttcccatcca gtgataagtc agggcaagcg tagcaaacac tgctattatt
      301 tttagcaaaa aaggcaatgt ttgatcctgt aactgcgtta tagcctgaac caggctaata
      361 ataatgccga caacagaggc cgctatgact gtaggcagtg agaggataaa tattatccaa
      421 agcgtttgca cacataattg aacaaaatat ccagtttcca tcaattcacc accaacagaa
      481 attcgaataa tttttgccag cctccaacaa gaatgaataa caagatctta aaaggaatcg
      541 aaattgttac tggcgatacc atcatcatac ccaaggccaa taagatattg gaaatgatca
      601 aatctatcgc aataaaaggt aaatagagca aaaaacctat cttga
//
LOCUS       DAZFRJ010000594          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000594 DAZFRJ010000000
VERSION     DAZFRJ010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.600"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..>641
                     /gene="yadN"
                     /locus_tag="ACHKUN_005336"
     CDS             61..>641
                     /gene="yadN"
                     /locus_tag="ACHKUN_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6663370.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITY"
BASE COUNT          182 a          137 c          138 g          184 t
ORIGIN      
        1 catgaatagt cattcttcaa accagtagtc tgactaatgg aattttattc aaggattttt
       61 atgtctaaaa aattaggttt tgccctgtct ggtcttatgc tggcaatggt tgcaggtact
      121 gcttctgctg atatggatgg tggtcagtta aatatcagtg gtctggttgt tgataacacc
      181 tgtgaaaccc gcgttgacgg cggtaataaa gatggtctga tcctgctgca aactgcaact
      241 gttggtgaaa tcgatgctgg cgtgctgaat gacaccgttg gcgctaaagc caaacctttc
      301 agcatcaccg ttgactgcag caaagcaaat ccgaatccag gtagtacagc taaaatgacc
      361 ttcggttctg ttttctttgg taatagcaaa ggaacgctga acaacgacat gtctattaac
      421 aacccgtctg acggcgtgaa tatcgctctg cacaatattg atggttccac tatcaaacag
      481 gttcaaatta acaaccctgg cgatgtgtat actaaagccc tggatgcaac gacaaaatct
      541 gctgtttatg attttaaagc gtcttacgtt cgtgccgtcg cagaccaaac agcaactgct
      601 ggttatgtaa aaactaacac tgcatacacc attacttatc a
//
LOCUS       DAZFRJ010000595          635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000595 DAZFRJ010000000
VERSION     DAZFRJ010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.601"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>635)
                     /locus_tag="ACHKUN_005337"
     CDS             complement(<1..>635)
                     /locus_tag="ACHKUN_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663371.1"
                     /translation="QRESEEQRLRDVYGDNPDALAKATSALKNTWSAEEQLRGSWMAG
                     LKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRGFTRSVLSMLTE
                     IFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATGGFTGTGGKYEPA
                     GIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSSAQMRRTEGI"
BASE COUNT          128 a          230 c          151 g          126 t
ORIGIN      
        1 taatgccttc cgtccgccgc atctgcgccg aacttccggc accgcccaca taaccacctt
       61 ccgcatagcc ccgcatcagg cggtacaggt tgccgacgcc aatccggctg gttgcttcct
      121 tcgtgaagac aaactccccg cgatggacaa taccggcagg ttcgtatttg ccaccggttc
      181 ccgtaaatcc tccggtcgcg aaatggaagt tcgccgccgc agcctgaatg gccgtccccg
      241 tggaggcaga cgcaccacca ccgaaagcac cgccaatggc gctgccgata ctcccgacta
      301 tccccaccat cgcctgcttc agaaaaatct ctgtcagcat ggagagcaca gaacgggtga
      361 aaccacgcca gttctgttcg ctgccggtca gcatcgctgc catattctgt gcaataccgt
      421 caaaggtctg cgtggccacg cttttaacct gcgaaaaact gtccgtcgca ctttctgccc
      481 actcgcccca gccggacttc agaccggcca tccagcttcc acgaagctgc tcctccgcag
      541 accaggtgtt cttcagtgca gatgtggcct tcgccagcgc atccggatta tcaccgtaca
      601 cgtcacgaag gcgctgctct tccgactccc gctgc
//
LOCUS       DAZFRJ010000596          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000596 DAZFRJ010000000
VERSION     DAZFRJ010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.602"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>633
                     /locus_tag="ACHKUN_005338"
     CDS             <1..>633
                     /locus_tag="ACHKUN_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO6663372.1"
                     /translation="ALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWYYDESD
                     RITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDKGRLTGERQTVENPE
                     TGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGDTPLLEYTRDR
                     MHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRDYGWNDNG"
BASE COUNT          151 a          184 c          206 g           92 t
ORIGIN      
        1 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
       61 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      121 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      181 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      241 gtccactacg gctacgacga taagggccgc ctgaccggcg aacggcagac ggtggagaac
      301 ccggagacgg gggaactgct gtggcagcat gagacaacac acgcctacaa cgaacagggg
      361 ctggcaaacc gcgtcacgcc ggacagcctg ccgccggtgg agtggctgac ctacggcagc
      421 ggttatctgg cgggcatgaa gcttggcgac acgccgctgc tggagtacac gcgcgaccgc
      481 atgcaccggg agacggtgcg cagctttggc agcatggcag gcagtaatgc cgcatataaa
      541 ctgaccagca catacacccc cgcaggccag ttacagagcc agcacctgaa cagcctggtg
      601 tatgaccgtg actacgggtg gaatgacaac ggc
//
LOCUS       DAZFRJ010000597          630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000597 DAZFRJ010000000
VERSION     DAZFRJ010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.603"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>630
                     /locus_tag="ACHKUN_005339"
     CDS             <1..>630
                     /locus_tag="ACHKUN_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6663373.1"
                     /translation="KRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAK
                     LELVRMNIIKQQGGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKL
                     GDCYPSKQGDTKDTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAE
                     DLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFR"
BASE COUNT          211 a          142 c          150 g          127 t
ORIGIN      
        1 aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca
       61 atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc
      121 aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt
      181 ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggagg ttcccctaaa
      241 atgagggaca tccctcaaaa cgagggaaaa tcccctaaaa cgagggataa aacatccctc
      301 aaattagggg attgctatcc ctcaaaacag ggggacacaa aagacactat tacaaaagaa
      361 aaaagaaaag attattcgtc cgagaattct ggcgaatcct ctgaccagcc agaaaacgat
      421 ctttctgtgg ttaaaccgga tgctgcaatt cagagcggca gcaagtgggg aacagcagaa
      481 gacctgaccg ccgcagagtg gatgtttgac atggtgaaga ccatcgcacc atcagccaga
      541 aaaccgaatt ttgcagggtg ggctaacgat atccgcctga tgcgtgaacg tgacggacgt
      601 aaccaccgcg atatgtgtgt gcttttccgc
//
LOCUS       DAZFRJ010000598          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000598 DAZFRJ010000000
VERSION     DAZFRJ010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.604"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>619
                     /locus_tag="ACHKUN_005340"
     rRNA            <1..>619
                     /locus_tag="ACHKUN_005340"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          142 a          138 c          201 g          138 t
ORIGIN      
        1 atggagccga ccttgaaata ccacccttta atgtttgatg ttctaacgtt gacccgtaat
       61 ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg cggtctcctc ctaaagagta
      121 acggaggagc acgaaggttg gctaatcctg gtcggacatc aggaggttag tgcaatggca
      181 taagccagct tgactgcgag cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat
      241 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
      301 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
      361 tcacatcctg gggctgaagt aggtcccaag ggtatggctg ttcgccattt aaagtggtac
      421 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgctggag
      481 aactgagggg ggctgctcct agtacgagag gaccggagtg gacgcatcac tggtgttcgg
      541 gttgtcatgc caatggcact gcccggtagc taaatgcgga agagataagt gctgaaagca
      601 tctaagcacg aaacttgcc
//
LOCUS       DAZFRJ010000599          617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000599 DAZFRJ010000000
VERSION     DAZFRJ010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.605"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..234
                     /locus_tag="ACHKUN_005341"
     CDS             <1..234
                     /locus_tag="ACHKUN_005341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663374.1"
                     /translation="HLVNRFVFFKISPIRQIAKWNNYSTKTFRYIAITCSPDIRSRYT
                     STPYSPVLTGSGGGIKRNKEQMAEQATMFVPPA"
BASE COUNT          193 a          147 c          124 g          153 t
ORIGIN      
        1 catttagtta atcgttttgt tttctttaag attagtccta ttagacaaat agcaaaatgg
       61 aataactaca gcactaagac gttccgctat atagctatta cctgttcccc tgacataagg
      121 agcagatata cctcaacccc ctattcccca gtgctgaccg ggagcggcgg cggtataaaa
      181 aggaacaagg aacaaatggc agaacaagca accatgtttg tcccccccgc ataaccgtac
      241 cattcgcatt aagaaatatt cccatatcta tcccgcaaaa gaggacccgt catacatcaa
      301 gtccgtttta ctgggcatca gattatcgcc gtaattagct cagtgaaagc cgggtaaacg
      361 gttagcaagc tcagtggctc aaaagtgcat agcgctaaga tgggcctggt tacacctgcc
      421 ctttgctgca gatcgtgttg atcggtgtga tgccgccaca cgtttaagtc aatctggaaa
      481 actcacaatg cgctcttcta taaagaggac tctttgataa agtaacccga aaagggaaag
      541 ctaactgata tcaatgtacc accttagtaa tcatccctta tatacagaca aaaagaagtt
      601 gcctgaaagc caggtat
//
LOCUS       DAZFRJ010000600          613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000600 DAZFRJ010000000
VERSION     DAZFRJ010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.606"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>613)
                     /locus_tag="ACHKUN_005342"
     CDS             complement(<1..>613)
                     /locus_tag="ACHKUN_005342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663375.1"
                     /translation="NMDMIPSYESMYLTLRGIPTDSIGGEDIYFIHDPQGGWYPNRGN
                     HLYAIDALCVNIHDALANSIFDGLENYHKFLYMAPEFLSTAGLNSESVVSKETFVLFI
                     DKFKGHTDVNKGLYLFDCCKIVSSIQECSKEVLQLQGEFYYTLNFEPLFFPNIEEEDG
                     IRYVTSPVVTKLFALLGFIYIRMYSLLDYITKLAIEIENLKTQ"
BASE COUNT          198 a          117 c          100 g          198 t
ORIGIN      
        1 aactgtgttt ttaaattttc aatttctata gctagttttg ttatgtagtc gagcaaactg
       61 tacatgcgga tataaataaa tccaagtaat gcaaagagct tagtcacgac tggagatgta
      121 acatatctta taccgtcctc ctcttcaatg ttagggaaaa acaagggttc aaaattcaac
      181 gtgtaataaa actccccttg tagttgcaac acttccttgc tgcattcctg tatgctactt
      241 acaattttac agcaatcaaa aaggtaaagg cctttattta cgtcagtatg ccctttgaat
      301 ttatcaataa ataaaacaaa tgtttctttt gaaactacgg attcagagtt caaacctgct
      361 gttgataaaa actcaggggc catataaagg aacttgtggt agttttctaa tccatcaaaa
      421 atgctattag ctagagcatc atgaatatta acgcataatg catcgatcgc ataaagatga
      481 ttccctctgt tcgggtacca tccaccttga ggatcatgta taaagtaaat gtcctcacct
      541 ccaattgagt cagttggtat ccctcttaaa gtcaaataca tgctttcata tgaaggtatc
      601 atatccatgt ttt
//
LOCUS       DAZFRJ010000601          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000601 DAZFRJ010000000
VERSION     DAZFRJ010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.607"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(5..>611)
                     /locus_tag="ACHKUN_005343"
     CDS             complement(5..>611)
                     /locus_tag="ACHKUN_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663376.1"
                     /translation="SAATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQA
                     MVGIVGRIGSAIGGAFGGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRG
                     EFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQND
                     GINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          127 a          225 c          134 g          125 t
ORIGIN      
        1 gttttcatcg cccgctccct gataacatac cgccatcacg caactgcagc cggagctcat
       61 cctgcgcccc cttgcgggcc atgtcataca ccgccttcat cagctgcggc cccgcctgtc
      121 cgttgatacc gtcgttctga atcaccacgt gattgttctg attaaaatta ataccttccg
      181 cccgccgcat ctgcgccgga cttccggcac cacccacata accaccttcc gcatagccgc
      241 gcatcagacg gtaaagattc cccacaccta tccggctggt tgcctctttc gtgaaaacaa
      301 actccccgcg gtgaactatc cccgcaggct catatttgcc gcccgtcccc gtaaatcctc
      361 cggtcgcgaa atggaagttc gccgccgcag cctcaatggc cgtccccgag gaagcagatg
      421 caccaccacc gaaagcaccg ccaatggcgc tgccgatacg cccgacaatg cccaccatgg
      481 cctgtttaag caggatttct gtcatcatgg acagcaccga acgggtgaat ccccgccagt
      541 ctgcctctgc accggtcagc atcgccgcca tattctgtgc aataccgtca aaggtctgcg
      601 tggcagcact t
//
LOCUS       DAZFRJ010000602          605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000602 DAZFRJ010000000
VERSION     DAZFRJ010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.608"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..166
                     /gene="xisR"
                     /locus_tag="ACHKUN_005344"
     CDS             <1..166
                     /gene="xisR"
                     /locus_tag="ACHKUN_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6663377.1"
                     /translation="LKKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQ
                     ASARPRRHLT"
     gene            186..>605
                     /locus_tag="ACHKUN_005345"
     CDS             186..>605
                     /locus_tag="ACHKUN_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO6663378.1"
                     /translation="MREVEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRR
                     VAGELRSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLEVRKE"
BASE COUNT          211 a          116 c          137 g          141 t
ORIGIN      
        1 gttaaaaaag gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca
       61 tgacggtatg ccatgggata acagtccatg cttttataac ctggaagaaa ttgatcgctg
      121 gattgagcgc caggcatcag cgagaccaag acgtcatctt acttgactaa aagccacact
      181 aactaatgag agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat
      241 tacgtatctg gtttgtttat aaaggcgtaa gagtcaggga aaatctgggg gttcctgaca
      301 catcaaaaaa caggcgcgtt gcaggtgaac tacgctcctc tgtttgttac gcaataaaaa
      361 ctggtgtttt cgactatgca aaacagtttc cctcctcacg caatctggaa aaatttggtg
      421 aggcccgaca agatttaacc ataaaagaac tggctgaaaa atttttggca ctgaaagaaa
      481 ctgaagtcgc caaaacatca ctcaacacat accgtgccgt catcaaaaat atcctgagca
      541 taatcggtga aaaaaatctt gcctcatcga ttaataaaga aaaattactg gaggttcgta
      601 aagag
//
LOCUS       DAZFRJ010000603          603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000603 DAZFRJ010000000
VERSION     DAZFRJ010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.609"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>603)
                     /locus_tag="ACHKUN_005346"
     CDS             complement(<1..>603)
                     /locus_tag="ACHKUN_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6663379.1"
                     /translation="FIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKAR"
BASE COUNT          124 a          196 c          157 g          126 t
ORIGIN      
        1 agcgcgcttt tgaaggtaac gtcaccacgc ggcgaacgat ggcctcttcc agccagcaca
       61 gaaacatctg gctcgcctga cgggatgcga cgaattttcg ccgccccata aagtacgccc
      121 acgactcgtt cgcactggcc cgtgccgtgg agtagctcat ctgggcgtaa ttccgggaaa
      181 gctgctcata cgagacaccc agcccggcag cgatataccg cagcagtgac tgctcaaaca
      241 cggagtagcc gttatccgtg tcctgagccg tctgcaggtt cagtgagtcc cccggcatca
      301 ggtgcggcac ttttgcgcct cccagacgga ccggtgctgc ggagtaatac gcggcaattt
      361 caccaatcca gccggtcagc ctttcccgct gctcctgact gttcgcgccc agaataaaat
      421 ccatcgctga ctgcgtatcc agctcactct caatggtggc ggcatacatc gccttcacaa
      481 tggcgctctg cagctgcgtg ttctgcagcg tgtcgagcat cttcatctgc tccatcacgc
      541 tgtaaaacac atttgcaccg cgggtctgcc cgtcctccac gggttcaaag acgtgaatga
      601 acg
//
LOCUS       DAZFRJ010000604          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000604 DAZFRJ010000000
VERSION     DAZFRJ010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.610"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>602
                     /locus_tag="ACHKUN_005347"
     CDS             <1..>602
                     /locus_tag="ACHKUN_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663380.1"
                     /translation="GQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAE
                     LRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYK
                     RQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRL
                     RDVYGDNPAALAKATSALKNTWSAEEQLRGSWMAGLKSGWG"
BASE COUNT          159 a          148 c          209 g           86 t
ORIGIN      
        1 gggcagcagg aagccgtcag ccgtctggct gaagagctga caggtaaaaa gcatacatca
       61 ccgaaaacgc gctctgccgg ggagagggaa gaggagcagg caagagaggc tctgcttgcc
      121 cttgaagctg agctcaggac gctggaaaaa cacagcggtg cgaatgagaa aatcagccgg
      181 cagcgccgtg atttatggaa ggcggaaagt cagtatgcgg tcctgaaaga ggctgccacg
      241 aaacgacagt tatctgagca ggaaaaatcc ctgctggcgc ataaagacga gacgctggag
      301 tacaaacgcc agctggctga gctgggcgac aaggttgaat accagaaacg cctgaatgag
      361 ctggcacagc aggcggtgcg gtttgaagag caacagagcg cgaagcaggc cgccatcagc
      421 gcaaaagccc gcggtctcac tgaccgtcag gcgcagcggg agtctgaagc gcagcgtctt
      481 cgggacgtgt acggtgataa tccggctgcg ctggcgaagg ccacatcggc actgaagaac
      541 acctggtctg cggaggagca gcttcgtgga agctggatgg ccgggctgaa gtccggctgg
      601 gg
//
LOCUS       DAZFRJ010000605          602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000605 DAZFRJ010000000
VERSION     DAZFRJ010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.611"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>602
                     /locus_tag="ACHKUN_005348"
     CDS             <1..>602
                     /locus_tag="ACHKUN_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663381.1"
                     /translation="GQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAE
                     LRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSWQEKSLLAHEKETLEYK
                     RQLADLGDKVEHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRL
                     RDVYGDNPQALARVTGALKQTWADEDMLRGDWLAGLKSGWG"
BASE COUNT          155 a          150 c          212 g           85 t
ORIGIN      
        1 gggcagcagg aagccgtcag ccgtctggct gaagagctga caggtaaaaa gcatacatca
       61 ccgaaaacgc gctctgccgg ggagagggaa gaggagcagg caagagaggc tctgcttgcc
      121 cttgaagctg agctcaggac gctggaaaaa cacagcggtg cgaatgagaa aatcagtcgg
      181 cagcgccgtg atttatggaa ggcggaaagt cagtatgcgg tcctgaaaga ggctgccacg
      241 aaacggcagt tatcctggca ggaaaaatcc ctgctggccc atgagaaaga gacgctggag
      301 tacaaacgcc agctggctga cctgggcgac aaggttgaac accagaaacg cctgaatgag
      361 ctggcacagc aggcggtgcg gtttgaagag cagcagagcg cgaagcaggc cgccatcagc
      421 gcaaaagccc gcggtctcac tgaccgtcag gcgcagcggg agtctgaagc gcagcgtctt
      481 cgggacgtgt acggtgataa tccgcaggcg ctggcccggg tcactggggc actgaaacag
      541 acatgggcgg atgaagacat gctgcgcggt gactggctgg ccgggctgaa gtccggctgg
      601 gg
//
LOCUS       DAZFRJ010000606          600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000606 DAZFRJ010000000
VERSION     DAZFRJ010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.612"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>600
                     /locus_tag="ACHKUN_005349"
     CDS             <1..>600
                     /locus_tag="ACHKUN_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase NikB"
                     /protein_id="HFO6663382.1"
                     /translation="IRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAA
                     CRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSY
                     RQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNV
                     AKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHHTDR"
BASE COUNT          147 a          127 c          186 g          140 t
ORIGIN      
        1 attcgtcgtg agcgccgtga agcccgtgca gctgcgcggg aagatttgcg tgcgcgttat
       61 cttgcatgga aggagcactg gcgtaaaccc gacttacgtt acggagaacg gttacgggaa
      121 attcatgctg cctgccgtcg gcgcaaggcg tatatccgtg tgcagttccg ggatccacaa
      181 ttgcgtaagc tgcattacca tattgcggaa gtgcagcgta tgcaggcgct gatcaggctg
      241 aaggagagtg tgaaggaaga gcggttgtca ctgattgcgg agggtaagtg gtatcctttg
      301 tcctaccggc agtgggtgga acaacaggcg gctcagggag acagggctgc agtatcgcag
      361 ttacgtggct gggattatcg tgatcgtcgc tcgcgtaata aggataaaag acgcacaact
      421 aatgttgatc gttgtgtcgt actctgcgaa cccggaggaa cgccactttt taataatgtt
      481 gcaaaacttg aagctcgtct tcagaaaaat ggaagtgttc atttcaggga tacgcggaca
      541 ggaaaaaatg tctgtacgga ttacggagat cgggttgtgt tctaccatca cacggaccga
//
LOCUS       DAZFRJ010000607          599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000607 DAZFRJ010000000
VERSION     DAZFRJ010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.613"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..220
                     /locus_tag="ACHKUN_005350"
     CDS             <1..220
                     /locus_tag="ACHKUN_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
                     /protein_id="HFO6663383.1"
                     /translation="AQEHLLLQCSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQI
                     LLPGEIHIKESGKREYKGRDSGLFMQPL"
     gene            complement(228..581)
                     /gene="cmi"
                     /locus_tag="ACHKUN_005351"
     CDS             complement(228..581)
                     /gene="cmi"
                     /locus_tag="ACHKUN_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO6663384.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
BASE COUNT          175 a          134 c          103 g          187 t
ORIGIN      
        1 agcacaggaa catcttcttt tacaatgttc atatgatgat aaatacgatt ttaacgccag
       61 cactcaccgt ggcatcatcg gagagtcgct cacaaggctc ggggcgatgt tttctggtaa
      121 agagtaccag atactgcttc ctggtgaaat tcacattaaa gaaagtggta agcgagaata
      181 taagggccgg gattccggcc tttttatgca gcctttataa cagtgtatta gtcattcgca
      241 tattcaacat atccctcctc acaatggtat atgccataat acattctctt accctccggg
      301 ccttcagctt taaatgggac aagaagtgtt ttttcatctt caacatctgt taaacttctc
      361 tcaatctttt cccatgtaat tttcgggatt tctgtaccaa gaagttgaac atcgttgtcc
      421 cagcgtttca ttctttgcag gtaatcattt tttacaaacg ttctggcctg ttcatcgctg
      481 acctgatagc atgccggacc tttatcttca gaccagaaaa aaacagcagc aagagcaata
      541 atcgttaaaa taaaaataaa tttcatgcta attactttca taaaatctcc acaacttct
//
LOCUS       DAZFRJ010000608          594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000608 DAZFRJ010000000
VERSION     DAZFRJ010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.614"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..83)
                     /locus_tag="ACHKUN_005352"
     CDS             complement(<1..83)
                     /locus_tag="ACHKUN_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6663385.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRR"
     gene            125..310
                     /locus_tag="ACHKUN_005353"
     CDS             125..310
                     /locus_tag="ACHKUN_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6663386.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(440..580)
                     /locus_tag="ACHKUN_005354"
     CDS             complement(440..580)
                     /locus_tag="ACHKUN_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663387.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
BASE COUNT          199 a          142 c          114 g          139 t
ORIGIN      
        1 cgacgctgtt cacccttcag aatattcacc cgtcgggtat tcagtgtttt gattctgctc
       61 tggagtgtac gaagctcagc catgtaaaat ccccgtcata tggcaatcag taaaggaaat
      121 aaatatgtca tcgaaaaacc ggacccgcag aaccacaacc cgcaatatcc gtttccccaa
      181 tcacatgatt gaacagatca atatcgccct tgaacataaa ggttccggta acttttcagc
      241 gtgggttatt gaagcctgca gaagaaggct gtcaacagag cgttcgggta tgaattacat
      301 aattaagtaa catggtgttc acagaacaca cagttaccgg acacatcagt tttccattcg
      361 tcccccggca gtacaggctt cccgtctgac gggatagcct gaaaaaacac agaaaattat
      421 ttgttataat taatataact tactcaaaaa aaagcgacga gaaaatcagc atcaacgagc
      481 aataagcgcc aatacgtgat aacaaatggc agccatattt atctgcagta taagcaatgg
      541 acaggataac cacaccagaa accgtcagca taaaatccat ttgaacttcc ccgg
//
LOCUS       DAZFRJ010000609          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000609 DAZFRJ010000000
VERSION     DAZFRJ010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.615"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..>593
                     /gene="espF"
                     /locus_tag="ACHKUN_005355"
     CDS             9..>593
                     /gene="espF"
                     /locus_tag="ACHKUN_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO6663388.1"
                     /translation="MLNGISQAVSTLGRHITSAVSRVSSAGLSGFSVSPQAVRLNPIR
                     AQSPFSPGTSNINARTTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKD
                     HLAAYEKSKSLDTSSLKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLAAYEKSKSL
                     DTSGLKPSRPAPPPPTSGQASEASRPLPPIAQALK"
BASE COUNT          135 a          174 c          139 g          145 t
ORIGIN      
        1 catgaattat gcttaatgga attagtcaag ctgtttctac actaggacgg catattacta
       61 gtgcggtaag ccgggtaagc tctgcggggt tatctggctt ttcagtgtcc cctcaggctg
      121 ttcgtctgaa tccgattagg gctcaatctc ctttttctcc cggtacgtcg aatatcaatg
      181 cgagaacgac ttttaatgtg agtagcccgg cgacttcatt tactccctct cgtccggccc
      241 caccgccgcc gacaagtgga caggcttccg gggcatcccg ccctttaccg cccattgctc
      301 aggcattaaa agatcattta gctgcctatg aaaagtcgaa aagtcttgat acttcaagcc
      361 ttaagccaag ccgcccggca ccgccgccgc cgacaagcgg gttggtatct gggtcatccc
      421 ggtctttacc gcccattgct caggcgctaa aagatcattt ggctgcctat gaaaaatcga
      481 aaagtcttga tacttcaggt cttaagccaa gccgcccggc accgccgcca ccgacaagtg
      541 gacaggcatc cgaggcatcc cggccattac cgcctattgc tcaggcatta aaa
//
LOCUS       DAZFRJ010000610          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000610 DAZFRJ010000000
VERSION     DAZFRJ010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.616"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..207
                     /locus_tag="ACHKUN_005356"
     CDS             <1..207
                     /locus_tag="ACHKUN_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6663389.1"
                     /translation="QALLPAIEKAMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDF
                     VASAVVLYDQMNRGGPAGNAVVMH"
     gene            230..>587
                     /locus_tag="ACHKUN_005357"
     CDS             230..>587
                     /locus_tag="ACHKUN_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6663390.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPL"
BASE COUNT          144 a          124 c          189 g          130 t
ORIGIN      
        1 caggctctgt taccggcgat cgaaaaggcg atgccgcctt tgctggtggc ccgtatgcgc
       61 agccacagtt ctgaatatta ccgtgagatc gtcgaacgga gggatcggct ggtgaaggat
      121 gtcgatgatt ttgttgcgtc agcggttgtt ttgtatgacc agatgaatcg cggcggcccg
      181 gcagggaatg ctgtggtgat gcactaaaag cacggtgttc gggggtttta tgagcagcaa
      241 gcttcatggt cttgtctggg aagggtgcgc cttcaccggc atgatcttat ccagggtggc
      301 ggttatggcc cgtcttgcag actacagcaa tgacgagggc gtgtcatggc ctgccattga
      361 aactatccgg cgtcagatcg gtgcaagaag tgaatccaca gtgaaatcgg ctattgcaga
      421 actggcgaaa gagggctggc tgacgaagga agagcgtaag gtcggtgggc gtaatgtaag
      481 caatatctat cggcttaatg tggaaaaact cgaagcagct gcggcggcgg cgcgtgagtc
      541 atataaaccg aaaagaaaaa ttagcccggc aaaaaatgac ccgttaa
//
LOCUS       DAZFRJ010000611          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000611 DAZFRJ010000000
VERSION     DAZFRJ010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.617"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..523
                     /locus_tag="ACHKUN_005358"
     CDS             <1..523
                     /locus_tag="ACHKUN_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO6663391.1"
                     /translation="ADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSAAVIKWYAE
                     RDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNARDSAEVVE
                     TAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDEL
                     IPGLLSEYIEQSG"
BASE COUNT          144 a          141 c          177 g          119 t
ORIGIN      
        1 ggctgacatt ttcggtgcga gtatccgtac cattcagaac tggcaggaac agggaatgcc
       61 cgttctgcga ggcggtggca agggtaatga ggtgctttat gactctgccg ccgtcataaa
      121 atggtatgcc gaaagggatg ctgaaattga gaacgaaaag ctgcgccggg aagttgaaga
      181 actgcggcag gccagcgaga cagatctcca gccagggact attgagtacg aacgccatcg
      241 acttacgcgt gcgcaggccg acgcacagga gctgaaaaat gccagagact ccgctgaagt
      301 ggtggaaacc gcattctgta ctttcgtgct gtcgcggatc gcaggtgaaa ttgccagtat
      361 tctcgacggg atccccctgt cggtgcagcg gcgttttccg gaactggaaa accgacatgt
      421 tgatttcctg aaacgggata tcatcaaagc catgaacaaa gcagccgcgc tggatgaact
      481 gataccgggg ttgctgagtg aatatatcga acagtcaggt taacaggctg cggcattttg
      541 tccgcgccgg gcttcgctca ctgttcaggc cggagccaca g
//
LOCUS       DAZFRJ010000612          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000612 DAZFRJ010000000
VERSION     DAZFRJ010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.618"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>580)
                     /locus_tag="ACHKUN_005359"
     CDS             complement(<1..>580)
                     /locus_tag="ACHKUN_005359"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6663392.1"
                     /translation="QPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHK
                     NLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVV
                     IPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDIL
                     KTLANPELTAVAKQGQDFVFEHYSWDGVTQRFE"
BASE COUNT          161 a          134 c          102 g          183 t
ORIGIN      
        1 ttcgaatctt tgagttacgc catcccaact gtagtgttca aacacaaagt cctgaccttg
       61 ctttgcaact gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga
      121 gtctgcagtc ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt
      181 catcccccca cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac
      241 catgcaaaat ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata
      301 acaatgcatc ttttcaggcg gtatgcttcc taacataata caacgatcct tcaaacgttt
      361 tgcaatttcc ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata
      421 atcaccaatc acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg
      481 catcaacagc aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc
      541 ttcaggggag atattaagtt cgcttctagt gatcggctga
//
LOCUS       DAZFRJ010000613          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000613 DAZFRJ010000000
VERSION     DAZFRJ010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.619"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHKUN_005360"
     CDS             <1..108
                     /locus_tag="ACHKUN_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663393.1"
                     /translation="EDAIKMRKYEPYLLNDNSILSRIALLELGIIGEQQ"
     gene            105..293
                     /locus_tag="ACHKUN_005361"
     CDS             105..293
                     /locus_tag="ACHKUN_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6663394.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            389..>578
                     /locus_tag="ACHKUN_005362"
     CDS             389..>578
                     /locus_tag="ACHKUN_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6663395.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCED"
BASE COUNT          178 a          130 c          126 g          144 t
ORIGIN      
        1 gaagatgcca ttaagatgag aaaatatgaa ccttatcttc tcaatgataa ttccatactt
       61 tccagaattg cccttcttga acttggtatt atcggagaac agcagtgact tcagcatttg
      121 cactggtgat gaccgttttt cttataacgg gtgagccaca aaatgtgatt accggaattt
      181 atgacagtaa gtcatcctgc attcaggtaa gggacgaaca aaaaatcccc ggtgaatgcc
      241 tcccgttaaa aaaagtatcg ctgaacctga ataacgaaat accggctgga taacccgcca
      301 gccatattaa cgccatacca acggattaaa aatgccagca atggcaggga ttcgttcacc
      361 ctgaaatctg taatgaggtt aaaacaaaat gagtaaagtc tttatttgcg ccgccattcc
      421 ggacgaacag gcaataaagg aagaaggtgc cgtcgctgta gccactgcca ttgaagccgg
      481 tgatgaacgt cgcgcccgcg caaaatttca ctggcaattc ctggaacatt atccggctgc
      541 tcaggactgc gcttataaat ttcttgtctg cgaggata
//
LOCUS       DAZFRJ010000614          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000614 DAZFRJ010000000
VERSION     DAZFRJ010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.620"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..576)
                     /locus_tag="ACHKUN_005363"
     CDS             complement(<1..576)
                     /locus_tag="ACHKUN_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO6663396.1"
                     /translation="MSNKIGQRVYTVVSEMSIDAWITKEPQPFVYRRSGAYQKLTIGS
                     DWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGV
                     PPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLY
                     YDVEVLFDWINDGQKFESIHPKGIIPEKIITK"
BASE COUNT          173 a          132 c          110 g          163 t
ORIGIN      
        1 tttggtgata attttttctg ggataatacc tttaggatgt atggattcaa acttctgacc
       61 atcattaatc cagtcgaaaa gcacttcaac atcataataa agggatttca gtagttgatt
      121 acatgtcgcg atatgaacat cagtaataat ccctccatta gtgacatagc caaataaatc
      181 gttgcacgca gcatcaatcc aaaactcgac ctcgtcattt tcactaacga gagaaaggtc
      241 aaccacccat ttacctggtt tatctggcgg tacgccataa gatgatgctt tattcgtgat
      301 gccgcagatg ggtgaaccat tcttatccac aataagacct tcgccaccga tatcaacgag
      361 agcgacgagt ttgtgtctaa atttatctgc tggacgttta ccataaactc gcatccaggc
      421 acagtcaaac agttgacccc agtctgagcc aatggtgagt ttctggtaag cgccgctacg
      481 gcgataaaca aaaggctgcg gttcttttgt tatccatgca tcaatcgaca tttcagagac
      541 aacagtataa acacgctgac ctattttatt cgacagtt
//
LOCUS       DAZFRJ010000615          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000615 DAZFRJ010000000
VERSION     DAZFRJ010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.621"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..>577
                     /gene="yibB"
                     /locus_tag="ACHKUN_005364"
     CDS             3..>577
                     /gene="yibB"
                     /locus_tag="ACHKUN_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO6663397.1"
                     /translation="MGRGDWTANKGFREKLARSVDVYFSYFERLAALENEMIIFTSPD
                     LKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNPEYWSP
                     EYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHL
                     FTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIV"
BASE COUNT          186 a           92 c          122 g          177 t
ORIGIN      
        1 acattggtag aggggattgg actgctaata aagggttccg tgaaaaacta gctcgttcag
       61 ttgatgttta ttttagttac tttgagcgtc tggccgcact tgaaaatgag atgattattt
      121 tcacttctcc cgatttgaaa ccaagggttg aagcgattcg caacggaaaa ccaacaacgg
      181 ttatcgttat cgatataaaa aagaaattta gatatatcag aagtcgtatc gaaaaaattc
      241 aaaaagatga gtcgtttaca aatagacttg agcctcgaca attaaaaaac ccagagtact
      301 ggtcaccaga gtatgttttg gtatgtaacc ttaaggccta ttttgtaaat aaagctatca
      361 acatgggctt agtgaaaacg cctcttgttg cgtggataga ttttggctat tgccgtaagc
      421 caaatgtaac tcgaggattg aaaatttggg atttcccatt tgatgaaagt aagatgcatc
      481 ttttcactat aaaaaaaggg cttactgtca catctcaaca acaggttttt gatttcatga
      541 ttggcaatca tgtatatatt attgggggcg caattgt
//
LOCUS       DAZFRJ010000616          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000616 DAZFRJ010000000
VERSION     DAZFRJ010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.622"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(81..326)
                     /locus_tag="ACHKUN_005365"
     CDS             complement(81..326)
                     /locus_tag="ACHKUN_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6663398.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            complement(367..546)
                     /locus_tag="ACHKUN_005366"
     CDS             complement(367..546)
                     /locus_tag="ACHKUN_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6663399.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
BASE COUNT          127 a          173 c          133 g          139 t
ORIGIN      
        1 cataccccgc ctcccgcaat ccggaagctc acaacaataa aaaagaccac cggcacacac
       61 cgatggtccc tgacgcatgc ttacatcatc atgtcgctgt ccggtgtggg gtcaccgcta
      121 tctgaagcac tcccctcacc cgcgatgcct tccggctccg gagctgccgg tgcgcccagc
      181 agttcatcca gaatggcatc cacttctgca tcaagacgcg cttccaggtt atggcgaagt
      241 ttctgtttca gtgcgctccg gacttcttca gagcgcagga cttccttcac tgcttcagca
      301 gtgaccaggg atgtaatttc tgacatggga ttttctcgtc gaaaggtgtg attaagaaag
      361 ttgccgctaa atgagcggct cttcgggttt gcttccggct gactgaccgg cgctgatttt
      421 ctcagcggcc cttttgtcaa tctgcctgcg ccagcaatcg cgcactgccc tgtacccacc
      481 cgaaaggaga tacagcacac agaccaccgt acagaagtac agcattaact ggttcagaaa
      541 tgtcataatt tctttccgtt attgttgaca at
//
LOCUS       DAZFRJ010000617          570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000617 DAZFRJ010000000
VERSION     DAZFRJ010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.623"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..502
                     /locus_tag="ACHKUN_005367"
     CDS             <1..502
                     /locus_tag="ACHKUN_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6663400.1"
                     /translation="CISHPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAATELSAIHGH
                     STSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDT
                     RKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLL
                     AGLPAH"
BASE COUNT          184 a          130 c          123 g          133 t
ORIGIN      
        1 ctgtattagc caccccaaga aaacaacgtt gcgtgataaa gccatggtga attatgcctt
       61 tgattattta agctcaccgg gcagtctacc gtttaccacg gcggccacgg agctttccgc
      121 gattcatggt cactcaacgt cgcaatatcg ccttggagaa ttttatcttc atggtagcga
      181 cggtaaacca ctggattata cacaggcgag atactggtat gagcaatcag cagaacagga
      241 aaatccgcgc gcacaaagca aactggggtg gatctacctc aaaggtctgg gggtcaaacc
      301 cgacacccgt aaagcaattc tctggtataa ggaagcagct gaacaagggt atgctcatgc
      361 tcaatatact ttaggtttga tctacagaaa tggctcaggt attaatgtta accattatga
      421 atctcaaaaa tggttaaaac tggccgccaa acaacattac aaaaatgcgg aaagattact
      481 tgccgggctt cccgcacatt aacgtcgact gtcaaccgaa cagatggagt attttcccta
      541 tcaaaccagc aaagataaaa atatagaaaa
//
LOCUS       DAZFRJ010000618          562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000618 DAZFRJ010000000
VERSION     DAZFRJ010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.624"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>562
                     /locus_tag="ACHKUN_005368"
     CDS             <1..>562
                     /locus_tag="ACHKUN_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6663401.1"
                     /translation="GIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPMNVNFDKL
                     SLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEINAMYPER
                     KLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASARVNQQNT
                     ENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT          170 a          135 c          138 g          119 t
ORIGIN      
        1 ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag gaaaaccgct
       61 gggatgagga atccgcttcc tttattccgg tcgaaccaga atccgatccg atgaacgtca
      121 attttgacaa gctgtccctt gaagtacaga acgcggtcct ggttaagttc ggtacatgtg
      181 aaaacatcac cgttgatatg gcgattgacg cgcaggaatt actgcaggaa gacgttgcta
      241 cctttgacgg gcatatcgtt gaagcactga tgaaaacgcc tgaaattaac gctatgtatc
      301 cggaacgcaa actgttcgct atcggatggg ttaaacacaa atgtaatccg ggtgccaaat
      361 ggcccgaaat tcaggctgaa ttacgtaact ggaaaaaacg gcaggacgca gagcgcaaag
      421 agactggaaa atacacgtct gttgttgatc tcgccagcgc cagagtcaat caacagaaca
      481 ctgaaaactc agcaggaaaa atcaacccag tcactgccgc catttgtcgc gaatacaagc
      541 agacatggaa aacgctggat ga
//
LOCUS       DAZFRJ010000619          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000619 DAZFRJ010000000
VERSION     DAZFRJ010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.625"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            390..470
                     /gene="shoB"
                     /locus_tag="ACHKUN_005369"
     CDS             390..470
                     /gene="shoB"
                     /locus_tag="ACHKUN_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO6663402.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT          164 a          115 c          116 g          163 t
ORIGIN      
        1 ccccgaactg gtaaggaacg ggggttttac tttttaactt taattttgtg cagcatgcac
       61 cctcaacaac agcgattatg gatcagtgta cttatctttt acaagcaact tgcaatcttt
      121 agcataaaaa ctcgagcctt tacgaagaaa gcaatattga tggaaagatt aacgtgaccg
      181 ccaattcgta agtacattaa aattggcttc gttattgaag attttgctgt gctttacacc
      241 atgccacaga attcccccat tgaaacgagt ggtgtcgtca aagctctggt gtggagtgca
      301 gcatgcaccc tcaataactc gcacgttcag ttttggggag atgtaagggc taatctgaac
      361 ggctgcattc cttgtttaag gaaaaacgaa tgactgattg ccgatacctg attaaacggg
      421 tcatcaaaat catcattgct gttttacagc tgatccttct gttcttataa cacaaggaaa
      481 cgtacttaag gtgcgtccgg tgaaccaatc ggacgcgcct ttaataacta taaataagtg
      541 tcttggcaga tactatat
//
LOCUS       DAZFRJ010000620          557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000620 DAZFRJ010000000
VERSION     DAZFRJ010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.626"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(6..140)
                     /locus_tag="ACHKUN_005370"
     CDS             complement(6..140)
                     /locus_tag="ACHKUN_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663403.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(216..527)
                     /locus_tag="ACHKUN_005371"
     CDS             complement(216..527)
                     /locus_tag="ACHKUN_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO6663404.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
BASE COUNT          137 a          141 c          121 g          158 t
ORIGIN      
        1 aatggctatt tcattgcgca taacgcctcc agttgtttct ttgctgctct aattaattgt
       61 ttaactcggc gtgataattc agattcgtgc gggtagaaag cggacatgac gccgctaccc
      121 gcgagctgaa agtgcatcat gggtaactcc ttatatttga ttgcataacg aaaacgcctc
      181 gagtgaagcg ttattggtat gcggtaaagc cgcgcttagg cggctgatgt ttcttctttc
      241 aggctttcga gatatttacg tgggtcgtcg taacattggc attcgctgta ccaatccacc
      301 cagcgatcag taagccccac ctctgataaa tcttcatcgg taaggctctc atcccacatc
      361 tcaaggccgt tagcattgca gtaatcaggc ttgatgttgt tgtcatactg aaaggcgtca
      421 taatcagcca gtgcgcccat caggcgaaca ccctcttcaa cacttgccac ttctgcaatg
      481 aacggtttca taggtacttg cgggatatgc cagacacgta atttcatatt tcctccgtca
      541 aaaaaatcgc cctcaca
//
LOCUS       DAZFRJ010000621          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000621 DAZFRJ010000000
VERSION     DAZFRJ010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.627"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..460
                     /locus_tag="ACHKUN_005372"
     CDS             <1..460
                     /locus_tag="ACHKUN_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6663405.1"
                     /translation="LRDAYDNDALYAFKVLFPSGKGFKFLAEVRQHTWSSGTNGVVAA
                     TFSLRMKGKPVSFVVPLAFVKNLDKTLTVNTGALLTMSVSANGGTPPYKYAWKKDGQP
                     VDGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSVECTVTVSAAAG"
     gene            427..>554
                     /locus_tag="ACHKUN_005373"
     CDS             427..>554
                     /locus_tag="ACHKUN_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6663406.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
BASE COUNT          134 a          125 c          178 g          117 t
ORIGIN      
        1 cctgcgtgat gcctatgaca atgacgcgtt gtatgcgttt aaggtgctgt ttccgtccgg
       61 taagggcttt aagttcctgg cggaagtgcg tcagcacacc tggtcatccg gtaccaacgg
      121 cgtggtggca gcaacgtttt cactgcgtat gaaaggcaaa ccggtgtcct ttgtggtacc
      181 gctggcgttt gtgaaaaatc tggataaaac acttaccgtg aatacaggtg cgctgctgac
      241 aatgtcagtc agtgccaacg ggggaacgcc gccgtataaa tacgcctgga agaaggatgg
      301 tcagccggtt gacgggcaga cgacagacac cttcagtaag ccaggtgcgc agtccgctga
      361 tgcggggaaa tatacctgcg tggtgaccga ttcggcagag aaagcacaga gtgtgacgtc
      421 tgttgaatgc accgtgacag tgagcgcagc cgccggataa ggggatgggt catcatgaaa
      481 aaggatctga aaacgctggc gctggccaga ctgtcagggt ttcgtcataa aacggtgaag
      541 gtgccggaat gggg
//
LOCUS       DAZFRJ010000622          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000622 DAZFRJ010000000
VERSION     DAZFRJ010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.628"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>550
                     /locus_tag="ACHKUN_005374"
     CDS             <1..>550
                     /locus_tag="ACHKUN_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6663407.1"
                     /translation="MQIAAEARLAATQERLNRNIAARSAAQNALNSTTAVGSRLMSGA
                     LGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSMSLPEASDNE
                     GRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMINHLTSVKTVT
                     EGLAQATEQLAVEQSRLAQMQEK"
BASE COUNT          141 a          137 c          178 g           94 t
ORIGIN      
        1 atgcagattg ctgcagaggc ccgtctggcg gccacacagg aacgcctgaa cagaaatatt
       61 gctgccagaa gcgccgccca gaatgcgctg aacagtacaa cggcggtggg ctcacgtctg
      121 atgagcggtg cgctggggct ggttggtggc gtacccggac tggtgatgct gggggctgca
      181 gcatggtaca cgctgtacca gaatcaggag caggccaggg agtctgcgcg ccagtatgca
      241 ctgacgatag atgaaatcgc gcataaaacg ccgtcaatgt ctttgcctga agcctcagat
      301 aatgaaggac gaacacgggc ggcgctgaca gagcagaacc ggctgattga tgaacaggcc
      361 agtcgggtga aatccctgca ggaaaaaatc gcaggatatc agtatgttct ggcgaacccg
      421 ggctggacga ccggtgacgg attcatgata aaccatctga cctcggtgaa gaccgtaacg
      481 gaagggcttg ctcaggcaac agagcagctt gccgttgagc agtcccgtct ggcacagatg
      541 caggaaaaag
//
LOCUS       DAZFRJ010000623          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000623 DAZFRJ010000000
VERSION     DAZFRJ010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.629"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>549)
                     /locus_tag="ACHKUN_005375"
     CDS             complement(<1..>549)
                     /locus_tag="ACHKUN_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663408.1"
                     /translation="QYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEH
                     RFSGLPLGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLA
                     VYDPTVQFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNT
                     VGKSAFVEAVGRASDDAEGYLDF"
BASE COUNT          117 a          168 c          149 g          115 t
ORIGIN      
        1 aaaatccagg taaccttccg catcatcgct cgcccgaccg acagcctcca cgaatgccga
       61 tttgccaaca gtattcacac tgcggatata aaaataataa tcatggcccg gtttgatatt
      121 gatactggca gctatccagt acagcgccga gccaagatag cgggctgcgg tttcaacctg
      181 cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg tcgggtcata
      241 aacggcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct caatccgcga
      301 cggtgctgcc ggtgcggcaa tccggaaggt ggtggtggca ggttcaccct gctggccata
      361 gctgtttatc gcccgcaccg tcagggtgta ttccccgagc ggcaggccgc tgaaacggtg
      421 ctccgtgtct gcggtgatgg cggtggtcac cagtctggca tccgttccct taccactggt
      481 caggcgcaga ctgaagcgca cgcccttcac cacccgcggc gtgtcccatt tagcctgcgc
      541 cagatactg
//
LOCUS       DAZFRJ010000624          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000624 DAZFRJ010000000
VERSION     DAZFRJ010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.630"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..>549
                     /locus_tag="ACHKUN_005376"
     CDS             90..>549
                     /locus_tag="ACHKUN_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663409.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIK"
BASE COUNT          203 a           96 c          100 g          150 t
ORIGIN      
        1 tatcacatta gcaccttggt taagggtgtt aataataaga ttttctcact tattaaaaca
       61 taaataattt tttaagcgga aatgtaatta tgagactgcc agagaaagtt ctttttcctc
      121 ctgtcactag tggcctgtca gggcaggaaa aacaaaaaaa accgaagagc attaccggat
      181 ttcaggaaaa ttatcaacgc aatatcaggc caatcaaaac agcatcagaa gcccgactac
      241 gcttctttga taaaatggtt tcgaaagaaa actctctgga agatgttgtt tctttaggtg
      301 aaatgattca gaaggaaatt tatgggcatg aacaaagaac attttcacca gttcatcata
      361 caggtaactg gaaatcatca ttgttacaca acgcgctcct tggtctggca aatgtttata
      421 atggcttacg ggaaacagaa taccctaaca ctttcaacag agatggtata aaaagtacta
      481 actcttttag agataactta ttgacaaaaa caagaactcc cagagataat tttgaggaag
      541 gaataaaac
//
LOCUS       DAZFRJ010000625          548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000625 DAZFRJ010000000
VERSION     DAZFRJ010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.631"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(235..454)
                     /locus_tag="ACHKUN_005377"
                     /pseudo
     CDS             complement(235..454)
                     /locus_tag="ACHKUN_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          151 a           98 c          129 g          170 t
ORIGIN      
        1 gtgtaattca cagacatctt ttcagttttt tctgttaatt ttctaattga tggactgtat
       61 acagggataa aaggagaggg tattgtctgg acatttctgt tagcataatt ggaaatgtgc
      121 gtgaaaaaaa gcataacaga catcatgtgt tatgtatgta ttcagagtgc ccataattca
      181 ccagtgcagg gataaattgt aaaaaaatcg cagtacgtcg tatgcagaac ggtgcggcgt
      241 acgcagttcc agtggaccgt cgcctgtgat aacggtcttt gtggaataac cgttgcggga
      301 gttagctcct gatctggact gatttttctc attcccaaga tggtgtgtca tctctgcatt
      361 gagagcggct tcaacgctga gctttttcag caaccgatca aactgactga ggtcttctga
      421 gggggctgag gtttttggcc agttcgttag caatacacca gaaaaattct aaacgagcct
      481 gtacatagat ttgtgtaatt gcctgatttt gatatgttca acccaacatc aaatgaaggt
      541 taatttat
//
LOCUS       DAZFRJ010000626          547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000626 DAZFRJ010000000
VERSION     DAZFRJ010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.632"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..520
                     /locus_tag="ACHKUN_005378"
     CDS             <1..520
                     /locus_tag="ACHKUN_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663410.1"
                     /translation="DTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRG
                     WVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPVTAIKVTPTSGTVAKGKTTT
                     LTVSFEPESATDKTFRAVSADPSTGTIAVKDMAITVTGVKAGKVSIPVISGNGQFATV
                     AEVTVTEAGAAG"
BASE COUNT          145 a          116 c          173 g          113 t
ORIGIN      
        1 agataccagt tttacgctgg cctggaaacc gggagaagaa ggtcagaaag ggcttatagg
       61 ctggtttgaa agcggggatg tgcgggccta taaaatccgt ttcccgaacg gcacggtgga
      121 tgtgttccgt ggctgggtca gcagtatcgg taaggccgta acggcgaagg aagtgatcac
      181 ccgtacggtg aaagtgacca acgtgggtaa accttctgtg gcggaagaac gcagcgaaat
      241 tacgccggtc actgcgatta aggtgacgcc gacatctggt acggtggcaa aagggaaaac
      301 aacaaccctg acggtttctt ttgagccgga aagtgcaacc gacaaaacgt tcagagcggt
      361 ttccgccgat ccgtcaacgg gaaccattgc tgtgaaagat atggcgatca ctgtgacggg
      421 ggttaaggct ggaaaagtga gtatccccgt gatttccggt aatggtcagt ttgccacggt
      481 agctgaagtc accgttactg aagcgggcgc tgcagggtaa acggaggtaa tacatgtttc
      541 tgaaaac
//
LOCUS       DAZFRJ010000627          539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000627 DAZFRJ010000000
VERSION     DAZFRJ010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.633"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(125..334)
                     /locus_tag="ACHKUN_005379"
     CDS             complement(125..334)
                     /locus_tag="ACHKUN_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6663411.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(430..>539)
                     /locus_tag="ACHKUN_005380"
     CDS             complement(430..>539)
                     /locus_tag="ACHKUN_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6663412.1"
                     /translation="PDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          119 a          178 c          125 g          117 t
ORIGIN      
        1 gcacgctgga gctccgactg gaatgactgt gaatttgccg aaattttgat aatcagttca
       61 cgtaacgtcg ccattcacct ttctccgggc aaaaaaacct gccacagcag gttttcatca
      121 ttatttatga cattgctgca aagctcagcg cgtcttccag cgccgcaaac ggatctgact
      181 ctactttatc ctcatcctcg ccccagcaga gcatggcgtc ctccagtgaa acattcatcc
      241 cctgcgcccc gaaaaccgct ttcacgatct gcgcattacg gatatccccg cgctcatcgc
      301 ccagcgggga caccctgtcg aactccatcc acatcatcgc ctcgctcgca ctcagactgt
      361 ggcccagttc ggataaggtg cgccccagac ggagcgcaag tcgcatcaga aagcgaattt
      421 ccgggcgggc tacttttttc tggccgactc tgcatcagcg atcagttcca gtgcctgacg
      481 cagcaaccgg gcatgtaccg gaccatagac ggccagcacc tgctcacggt cgtccggag
//
LOCUS       DAZFRJ010000628          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000628 DAZFRJ010000000
VERSION     DAZFRJ010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.634"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>538
                     /gene="escD"
                     /locus_tag="ACHKUN_005381"
     CDS             <1..>538
                     /gene="escD"
                     /locus_tag="ACHKUN_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO6663413.1"
                     /translation="AINDILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLL
                     LNTPGVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKI
                     LAIINAMNKNSNVKILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSI
                     LKGGYVIDAIDPKDGINIS"
BASE COUNT          194 a           97 c          107 g          140 t
ORIGIN      
        1 cgcgattaat gatattttaa cggaatacgg ttataaggat atcatcatta caaaaggtaa
       61 taagcctgga ttcttcctgt tatcgggata cattccccct tcaccaaaat ggagcgaagt
      121 tgaaaatctg ttactgaata cgccaggggt tgctggatgg gaaatacata acaacagtaa
      181 caataaaatc aatgaactgg catcagagtt caaaaagaat aaattaataa actatgtcaa
      241 tattttcaaa aagaatgatg tcatcattgt tgcgggggag gtttctcaac agaacgagag
      301 taagatcctt gccattataa atgcgatgaa caaaaatagt aacgtcaaga tactatttca
      361 gaacattcag ccgtacattt cagcagatat tttccccgga aaaatactca gaatcagcgg
      421 cacgatgaag aatccaacaa ttgctcttga taacggtaca agcctgggaa ttggtagcat
      481 tctgaaagga ggctatgtca tagacgcgat tgatcctaaa gatggtatca acatctcc
//
LOCUS       DAZFRJ010000629          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000629 DAZFRJ010000000
VERSION     DAZFRJ010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.635"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..>532)
                     /locus_tag="ACHKUN_005382"
     CDS             complement(116..>532)
                     /locus_tag="ACHKUN_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO6663414.1"
                     /translation="GNNAALYDLYRFRSALSACRSLGWQIGTCAWNDQLLSETLSRAQ
                     TGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHPASLESSEVEEIFRDEGFILAHGREC
                     SLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNHLLH"
BASE COUNT          127 a          132 c          143 g          130 t
ORIGIN      
        1 ctttatgaca accggagagg acggataata aaaaatggga catccacatc agcaaaccgc
       61 gatttgcgaa aaaaagtcag cggagagtgg ctgccgttgt ggctgtcccg gtatttcagt
      121 gtaacaggtg attgctgtga tctatgcccc attcggcttt cggtaaacct gaatgcccca
      181 catgaagaat gcgtgacagc atgtttctgc cgctgccgtt caggctgcat tcgcgtccgt
      241 gcgcgagtat aaaaccttca tcccggaata tctcttccac ttctgaagat tcgagggatg
      301 ccggatgaat taatgaaaat gccaccggtc tgagcattct gcccgtggtg gaaaaggtgt
      361 ctctggtact gttcagctgc aggatatgac gtttacctgt ttgcgcacgg gaaagtgtct
      421 cactcagcag ctggtcgttc catgcgcacg tgccaatctg ccatcccaga cttctgcacg
      481 cggaaagagc cgagcggaag cggtacaggt cgtataatgc tgcgttattg cc
//
LOCUS       DAZFRJ010000630          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000630 DAZFRJ010000000
VERSION     DAZFRJ010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.636"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            129..317
                     /locus_tag="ACHKUN_005383"
     CDS             129..317
                     /locus_tag="ACHKUN_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6663415.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            375..>526
                     /locus_tag="ACHKUN_005384"
     CDS             375..>526
                     /locus_tag="ACHKUN_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663416.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCV"
BASE COUNT          156 a          133 c          121 g          116 t
ORIGIN      
        1 gattattcct ctttctgtaa accatacggc ctcaacattt gccaactctg aatcatcctg
       61 acacaatcag acacatccca acgcaataga acacgaaata actctttaag aaacgaaagg
      121 gggcaatagt gttaagcact gatcggttta tacgtgaaaa agaatgcgaa aagctaacag
      181 gccttagccg ctcatgccgc taccgcctgg aaaaggccgg acaattccca tcacgtcgta
      241 aacttggcgg tcgttccgtt ggctggtctt tatccgaggt tctggcctgg aaggatagct
      301 gcaaggcagt tcattaatca cgctggcggc acgcagccgc cacacatcaa tcatctgaac
      361 acagagccat aaccatgaag attgaatata cgccagaacg tgggcgggga ttcgttcgcc
      421 ctggtgaaac tgaaaaacaa caaaattggg gtttttcagg tataaaaaaa gcggccccga
      481 aatggagccg cctttctgaa cagataaccc gctgcgccgt ttgtgt
//
LOCUS       DAZFRJ010000631          516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000631 DAZFRJ010000000
VERSION     DAZFRJ010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.637"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>516)
                     /gene="sctD"
                     /locus_tag="ACHKUN_005385"
     CDS             complement(<1..>516)
                     /gene="sctD"
                     /locus_tag="ACHKUN_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HFO6663417.1"
                     /translation="NRELQLPMGNLTIGTEDNDIVYFPLEQGLNQFLLDIREEGVFLL
                     SPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLPLSDVPERFSAKNRRK
                     KRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQLKENKLHAITLVWHGK
                     NVALYGRCESTT"
BASE COUNT          146 a          117 c          116 g          137 t
ORIGIN      
        1 agtggttgat tcgcaacgcc cataaagcgc gacatttttc ccatgccata ccagagtgat
       61 tgcatgaagt ttgttttctt ttagctgctg gtatacatca gctctggtaa acgctggggg
      121 ttcagctttt ggggaaagca aaacgtaact gccgatggcg ccagacaacg caatcgcagc
      181 accaatcacg ctagccagga taagacgctt tttacgtcga tttttggcag agaacctttc
      241 tggcacatca gacaatggca aggtgtggtc aatatcacca atgatgaagc aacatcccgc
      301 tatatcgatg acctttccga caggtaatga ctgatctgct tcataggggg tgggctgtcc
      361 atcaatccaa aattcaaccg gagataaaag gaaaacacct tcctcacgta tatcaagtaa
      421 aaactgattg aggccttgtt caaggggaaa ataaacaata tcgttatctt ctgtgcctat
      481 cgttaggttt cccatcggaa gttgtaattc ccgatt
//
LOCUS       DAZFRJ010000632          514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000632 DAZFRJ010000000
VERSION     DAZFRJ010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.638"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          202 a          113 c           70 g          129 t
ORIGIN      
        1 ataaataaca aaaaccattt aatactgata catcaataaa atacttcttc cctgctgaca
       61 cagacaaaat gcaccatttt ttcttcaaat acttctgtat aatcgccagc caatgatgag
      121 aatggattaa aataacactt cttcgacaaa cacaaaaaca gccttcattt tacatcatca
      181 tccagtttca gttaaccccg gacgagcgac agtgtttcag tacaggaatt aaaagccagc
      241 ttttgctcag taaaaacaca ctcaactaat aatatctcag cagatattat cctgaccagg
      301 acacctcaca ttactgaatt ctcttccgac agtatcactg cacaaataaa aaagaacggg
      361 ataaatattc agaccggaag aatggtgtca caaaagcatt taatcattac gcatgaccag
      421 gaagggaaac acttaaccac atggtatcaa cagatatcat tacgtgtgca ctcttataaa
      481 aaacacacag aagaagtaag ataattataa acgt
//
LOCUS       DAZFRJ010000633          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000633 DAZFRJ010000000
VERSION     DAZFRJ010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.639"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHKUN_005386"
     CDS             complement(<1..176)
                     /locus_tag="ACHKUN_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6663418.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYE"
     gene            complement(244..>513)
                     /locus_tag="ACHKUN_005387"
     CDS             complement(244..>513)
                     /locus_tag="ACHKUN_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663419.1"
                     /translation="GAKHEREHTDIYSSCRLIVRKNGAEIYNCTALDNTLIYSGVIDM
                     PAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          134 a          134 c          113 g          132 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatcccg ttaagattat cgctaccggg agcgttcgtc
       61 cgggcatgaa gataccccgc gctcagcgtg gcctgctgct cagacgccca tgcaggcgca
      121 ccggatacgg tcagacaaat ggctgcggac aaaatggcgg cataaagttt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaaggc gtcatttctg acgcccgttc tgggttataa
      241 aattcagctg atactgatac ctgctgtgga ttttttcatc accacaacca gcagatcgct
      301 gatacttgct gtgggatacc agttatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacag ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagta
      481 aatatcagta tgctctctct catgtttagc gcc
//
LOCUS       DAZFRJ010000634          510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000634 DAZFRJ010000000
VERSION     DAZFRJ010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.640"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..>510
                     /locus_tag="ACHKUN_005388"
     CDS             92..>510
                     /locus_tag="ACHKUN_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6663420.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRK"
BASE COUNT          166 a          106 c          107 g          131 t
ORIGIN      
        1 agaaaaatca tcttctcagg gacgtataag actgtgcttt atgcaaagca ctatcaggaa
       61 acatttatcc cttgctaagg taaactcaac tatgagtatt attatcaaca actggcggat
      121 ggacccgtcg cttaatgcct taatacattg cgaaacgggt gaaacacgtc gtctcggcga
      181 ataccatttt attttgctgg aaaaattagc aaaaaatgcc gatgtggttt tatcgcgatc
      241 ttatttatgt gccgaagtat ggaagaaccg cattgtcggc ggaaacagcc tgccgacggc
      301 gatccatgcg ttgcgcgttg ctatcgatga tgatggcaaa caacagaaca ttattaaaac
      361 aattccgaag aaaggttact tatgcaataa agagtatgtg tctttgccgg actcatcgcc
      421 tgcaaaaaaa ctgattatta ccgaccagat acaggaaaca gtaccagaag agatatcttc
      481 tacaacaccg cctgtgcctg ttagaaaaaa
//
LOCUS       DAZFRJ010000635          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000635 DAZFRJ010000000
VERSION     DAZFRJ010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.641"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..>508
                     /locus_tag="ACHKUN_005389"
     CDS             12..>508
                     /locus_tag="ACHKUN_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO6663421.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLAS"
BASE COUNT          129 a          129 c          162 g           88 t
ORIGIN      
        1 gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaagata
       61 acctgaaatc cacgcagctg ctgagtgtga ttgatgccat cagcgaaggg ccgattgaag
      121 gtccggtgga tggattaaaa agcgtgctgc tgaacagtac gccggtgctg gacagtgagg
      181 ggaataccaa catctccggc gtcacggtgg tgttccgggc aggtgagcag gagcagacac
      241 cgccggaagg ttttgaatcc tccggctccg agacggtgct gggtacggaa gtgaaatatg
      301 acacgccgat cacccgcacc atcacgtcgg caaacatcga ccgtctgcgc tttaccttcg
      361 gtgtgcaggc actggtggaa accacctcaa agggggaccg gaatccgtcg gaagtccgcc
      421 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
      481 gcaaaaccac ctcgcagtat ctggcctc
//
LOCUS       DAZFRJ010000636          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000636 DAZFRJ010000000
VERSION     DAZFRJ010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.642"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>497
                     /locus_tag="ACHKUN_005390"
     CDS             <1..>497
                     /locus_tag="ACHKUN_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO6663422.1"
                     /translation="LKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVL
                     LNDENNELSDDTNFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHL
                     EDALNENIKSDPSWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRAL
                     GHDKE"
BASE COUNT          181 a          104 c           79 g          133 t
ORIGIN      
        1 accttaaacc aactggtccg gataattcag atgttctcct gttcaaaaag ctattctccc
       61 cttcgaaatt aatgacaatt atcggtgatg aaattcctct tatatctgaa aaacagtcgc
      121 tttcaaaggt gcttttaaat gatgagaata atgaactgag tgatgataca aacttctggg
      181 ataaaaatcg tcaattaacc acagatgaaa tagcttgcta tcttcagaag atcgccgcta
      241 atgcaaaaaa tactcaagtc aattatccta ctggtctcta cgtcccctac tccaccagaa
      301 ctcacctgga agacgctctc aatgaaaata ttaagagcga tccatcatgg ccgaatgaag
      361 tccagttatt ccacataaat actggcggac actggatatt agtttcgcta cagaaaatag
      421 taaataaaaa aaataataaa ctacaaataa aatgcgtcat attcaactca ttgcgtgcac
      481 taggccatga taaagaa
//
LOCUS       DAZFRJ010000637          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000637 DAZFRJ010000000
VERSION     DAZFRJ010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.643"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>497
                     /locus_tag="ACHKUN_005391"
     CDS             <1..>497
                     /locus_tag="ACHKUN_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6663423.1"
                     /translation="AKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNVRV
                     ISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFV
                     AMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKLEV
                     NAAQK"
BASE COUNT          149 a           97 c          102 g          149 t
ORIGIN      
        1 gcgaaaatta atctcttatc acggcttacg ccgcttttat tcgtgttcgc tccttttttg
       61 gcacagtcaa atatgactgt ttatcctatg gctgtttcca ttaatagtca gggtgaaggc
      121 aacgttcgcg taatatctaa aagtaatgaa gttcagtaca tcaaggcgac agtattccgt
      181 atcgataatc catcgacgcc tcaggaaaat gaagttgaga ttaaatccgg tgatgcaaat
      241 catctggttg ttatgccacc taaattcgct ttaccagccg gtagtagcaa aaccgtacgt
      301 tttgttgcga tggaaccaga gcaaaaagag aaaaattatc gcgttaaatt tgaagcggtt
      361 cccagtattg atgacgttgc cacagataaa aaagatctct ctatgcagtt aacagttaac
      421 ttaatttggg ggattgttgt tagtgttcca cctcagcaac ctattgctaa gttagaagta
      481 aatgctgccc aaaaatt
//
LOCUS       DAZFRJ010000638          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000638 DAZFRJ010000000
VERSION     DAZFRJ010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.644"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>494
                     /locus_tag="ACHKUN_005392"
     CDS             <1..>494
                     /locus_tag="ACHKUN_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6663424.1"
                     /translation="GEAAAQGWSIKDGGIQLSDGVFKITKQSNKTWSLTHPVDDAITL
                     LTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDG
                     RVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSL
                     TLGL"
BASE COUNT          117 a          121 c          152 g          104 t
ORIGIN      
        1 ccggagaggc tgctgcgcag ggctggagca ttaaggatgg cggaattcag ttgtcagatg
       61 gtgtatttaa gatcaccaag cagagcaata aaacctggtc cctgacgcat ccggtggatg
      121 acgcaattac cctgctgaca cagggcggca gactgacctg taagttccgc ctgtcaggcg
      181 cactgaccaa caatcagttc gggctgggga tttatctgta tacggatgct cccgttcctg
      241 atggtgtggc gatgacgggt accggtaatc cgttcctgat gtcgtacttc actcagacca
      301 ctgacggcag agtgaatctg atgcatcaca ggaaagccgg aaacacgaag ctgggggagt
      361 tcggcgatta cggtaacgac tggcagacgc tggagctggt gttcaccgcc ggcagtgcca
      421 cggttactcc gaaactgaat ggagtggctg gcccggcatt ccaggttata aaagacagtc
      481 tgacactggg actg
//
LOCUS       DAZFRJ010000639          493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000639 DAZFRJ010000000
VERSION     DAZFRJ010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.645"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            373..>493
                     /locus_tag="ACHKUN_005393"
     CDS             373..>493
                     /locus_tag="ACHKUN_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663425.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAF"
BASE COUNT          150 a          123 c          112 g          108 t
ORIGIN      
        1 ggagtaaaac tgataccgcc aaacgtcacc agaaaattga taacagaggg cgttgcagcg
       61 gggttgtcac ttaagcgtat ggtcaacctg acaacccggt gtcctcaatg gggaaggaat
      121 aaccccgcca tacttaccgc cgcgccattt cgcggagtgc cacaaccgga agcgcacgtt
      181 cgaagaaatc taacgacaag ccttctaagg gaaagagctt cgccgtacgc tttcgcgtta
      241 tgccctgact tttcagggaa atatcctttc agtaaactgt cagtaccgga ttcttatccg
      301 tgtccggcgc acgaccacac gtgacagcgt gttggtctcc atttttaacc cagaacctca
      361 atggaggata aaatgccaaa caaaaaaaga aatccgctta ttgaaaaaca gattgaatgc
      421 ctggtaaatc aactcaggca atcagggtta ttaaaaactc attcagagtt gaggctcaca
      481 gaatcagcat tcg
//
LOCUS       DAZFRJ010000640          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000640 DAZFRJ010000000
VERSION     DAZFRJ010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.646"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..491
                     /locus_tag="ACHKUN_005394"
                     /pseudo
     CDS             1..491
                     /locus_tag="ACHKUN_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          156 a           77 c          130 g          128 t
ORIGIN      
        1 aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga cccgataggg
       61 ctggaggggg ggatggagtc tgtatgcgta cccgctgaat ccggtgaatg gtattgatcc
      121 attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac taaaccatca
      181 aagagcatgg gatatattat ctgatactta tgaagatatg aagagattaa atttaggtgg
      241 gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg
      301 tgttagccga tcggcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa
      361 tctgttcggt atgtacggca gaaaagtaaa gctatcccat tctgaaatga ttgaagataa
      421 taaaaaagac ttggctgtaa atgaccatgg gttgacatgt ccatcaacaa cagattgctc
      481 agatagatgt a
//
LOCUS       DAZFRJ010000641          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000641 DAZFRJ010000000
VERSION     DAZFRJ010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.647"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>485
                     /locus_tag="ACHKUN_005395"
     rRNA            <1..>485
                     /locus_tag="ACHKUN_005395"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZFRJ010000642          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000642 DAZFRJ010000000
VERSION     DAZFRJ010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.648"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHKUN_005396"
     rRNA            <1..>476
                     /locus_tag="ACHKUN_005396"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFRJ010000643          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000643 DAZFRJ010000000
VERSION     DAZFRJ010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.649"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>475)
                     /locus_tag="ACHKUN_005397"
     CDS             complement(<1..>475)
                     /locus_tag="ACHKUN_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6663426.1"
                     /translation="TYEIWFYVVFFFSLCISHKYRTAIASFLLIIICVLGQLYFHGDV
                     FLNPIAQPPSGDGIIRNLVFVSNPIIYDFILGMIIAEAYIRLPSRLINKMPVVQFLNI
                     TLAIALLFLCLGLRNGPGIVAWGAYSFMIIAPIVLISKCRDVYRNKILTYVGEIS"
BASE COUNT          184 a           87 c           76 g          128 t
ORIGIN      
        1 taggatattt caccaacata cgttagaatt ttatttctat atacatccct acacttagaa
       61 ataaggacta ttggggctat aatcataaat gaataagccc cccatgctac aattccaggt
      121 ccattcctta accctagaca taaaaaaagc aaggcaatcg ctaatgtaat attaagaaat
      181 tgaacaacag gcattttatt tataagtcta gaaggtagcc taatgtaggc ctctgcaata
      241 atcatcccca aaataaagtc atatattatt ggatttgata caaataccag attacgaata
      301 attccatcac cagatggcgg ttgcgcgata ggatttagaa aaacatctcc gtgaaagtac
      361 agttgaccaa gaacgcaaat aatgatgaga agaaatgatg cgatcgcagt tctatattta
      421 tggctgatgc ataatgaaaa aaagaaaacc acatagaacc aaatttcata cgtta
//
LOCUS       DAZFRJ010000644          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000644 DAZFRJ010000000
VERSION     DAZFRJ010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.650"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>472)
                     /gene="nleC"
                     /locus_tag="ACHKUN_005398"
     CDS             complement(<1..>472)
                     /gene="nleC"
                     /locus_tag="ACHKUN_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO6663427.1"
                     /translation="RLIDQHTVDMIGNTVLDALSRSQTFRDAVSYGIHNEEVHIGCIK
                     YRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEAGENENEEPYVSFSVAP
                     DTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEILARRVAQELGWSVP"
BASE COUNT          120 a          117 c           96 g          139 t
ORIGIN      
        1 ggaacactcc atcccagctc ttgagcgaca cgacgtgcga gaatctcggt gggtcctagc
       61 tctatattac tatctccaga tggatcgctg gccccagtaa catgatgaat aatctcgtga
      121 atcagtcctt cctgccacga tggcatctca taagaatcag tatctggcgc aacactaaaa
      181 ctgacataag gctcttcgtt ctcgttttct cctgcctcgc aaatggggag aattggttct
      241 tgcccgacat cgtatccata taattcgtta caggttagtg attgaatatc atcaattttg
      301 atagcagatt ctccgttaag ctcgtattcg tttctgtatt taatgcaacc aatgtgtacc
      361 tcctcattat gaatgccata gcttacggca tcacgaaatg tttgtgatcg gctcaacgca
      421 tcaagtacag tgttgccaat catatcgacc gtatgctgat cgatgagcct gc
//
LOCUS       DAZFRJ010000645          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000645 DAZFRJ010000000
VERSION     DAZFRJ010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.651"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            69..293
                     /locus_tag="ACHKUN_005399"
     CDS             69..293
                     /locus_tag="ACHKUN_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663428.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
BASE COUNT          122 a          104 c          127 g          117 t
ORIGIN      
        1 gtggaagggg cacaggacta tatccgcact cagtgtacta agcaggcttt ttattatccg
       61 gaggatgtat gaagaaatta caggtaacgg taaaaccttt tcagggaaca attccgttcc
      121 gtgttttgca acatggccgt gttctgcttg aagaggtgtt cagaggtaaa tgcactgaat
      181 gttattcacg aacatatgaa gtgaatgcca cgcatgaaga attcaccgtt gagtgtgtga
      241 tgaatactga taaatgccga atggtatccg ctgaattaca gccagtgtgt tgagcgacct
      301 tattatccat gcgcggtatt gtcgccgtat tcctgcatta acagagaccg cagcccgaca
      361 gggagactcc tctgcgagag tgtgcgggga taatcaaaaa cgatacacac cggggtttac
      421 cgcgtaaacg gagcgcggcg ttctcccctc atggtcgccc gtccggtgcg
//
LOCUS       DAZFRJ010000646          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000646 DAZFRJ010000000
VERSION     DAZFRJ010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.652"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..470
                     /locus_tag="ACHKUN_005400"
                     /pseudo
     CDS             2..470
                     /locus_tag="ACHKUN_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          183 a           99 c           69 g          119 t
ORIGIN      
        1 tatttataac tgcacaacaa ataccgataa tgctaaaacg ccagacacac cgaagtggcc
       61 ttgcgataat tgggaagaac aataacccat taattccaca ttctctggag aaggatatat
      121 ctctgatcaa tatgatttag cgcaacacca acttcaacaa attaatgcgt gccacacgaa
      181 cactacatat acaaatgcag actactcaaa agttgtggct caacttgtaa gtcttattac
      241 taacattgaa acgataagtt cgacacaact tacgcaacag acacaatcaa tacttaatca
      301 gattaataac attagatatg aaaaaaacaa aagtgctgaa tgtcgaatca ttgttgtcgc
      361 taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg gaatacccat
      421 aacattcagt gtccaaacca tgttttcaga tactaatttt attgctgaac
//
LOCUS       DAZFRJ010000647          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000647 DAZFRJ010000000
VERSION     DAZFRJ010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.653"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..285)
                     /locus_tag="ACHKUN_005401"
     CDS             complement(<1..285)
                     /locus_tag="ACHKUN_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663429.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHD"
BASE COUNT          144 a           89 c           96 g          140 t
ORIGIN      
        1 gtcatgattc aggtaatatg tagagatagt cccgttaaca gtgtcaccat taaaggtggt
       61 atcgaaatca ccgtttgtgt tgttatagta gacaaagcca ctataattca catagttacc
      121 atctacagta cccggtgcat ccatattgct ccagtcaaga gcggtagttg gattgtcgta
      181 ataagtccag gtgtcataag tagttgcatt agcctgagag gtgatagcca gagtgcaagc
      241 caatgctaat tgtgatacta caagtttctt tttccaggag tgcatttgtc atccctcctc
      301 agggacgtaa taagttgatc catcaattat caatgcatag aaaacatatt atgtttcgcg
      361 ctaatgatta tgctgcgtta cagacgaatg gttcaattat tctttcccct aagtccgaat
      421 gtggacatat ataacctatg gagaaaaaca cgcatcaaat tgataaaag
//
LOCUS       DAZFRJ010000648          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000648 DAZFRJ010000000
VERSION     DAZFRJ010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.654"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..>467
                     /locus_tag="ACHKUN_005402"
     CDS             106..>467
                     /locus_tag="ACHKUN_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO6663430.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT          133 a          117 c          105 g          112 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZFRJ010000649          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000649 DAZFRJ010000000
VERSION     DAZFRJ010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.655"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..>459
                     /gene="gadE"
                     /locus_tag="ACHKUN_005403"
     CDS             155..>459
                     /gene="gadE"
                     /locus_tag="ACHKUN_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO6663431.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     "
BASE COUNT          166 a           69 c           86 g          138 t
ORIGIN      
        1 taaatctgaa gtattgatta gtgttaatag acgtattagt tcacgaaggg caaagttctt
       61 ataggcgttt actatattga acaacgattc ggacaaggat gtaaataatg aaaaggatga
      121 catgttcgaa acgataacgg ctaaggagca agttatgatt tttctcatga cgaaagattc
      181 ttttctttta cagggctttt ggcagttgaa agataatcac gaaatgataa aaatcaattc
      241 cctgtcagag atcaaaaaag taggcaataa acccttcaag gttatcattg atacctatca
      301 caatcatatc cttgatgaag aagcgattaa atttctggag aaattagatg ccgagagaat
      361 tattgttttg gcaccttatc acatcagtaa actaaaagct aaagcgccta tttattttgt
      421 tagccgcaaa gaaagtatca aaaatcttct tgagattac
//
LOCUS       DAZFRJ010000650          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000650 DAZFRJ010000000
VERSION     DAZFRJ010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.656"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..139)
                     /locus_tag="ACHKUN_005404"
     CDS             complement(<1..139)
                     /locus_tag="ACHKUN_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663432.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DV"
     gene            157..348
                     /gene="yfgG"
                     /locus_tag="ACHKUN_005405"
     CDS             157..348
                     /gene="yfgG"
                     /locus_tag="ACHKUN_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO6663433.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          124 a          115 c           78 g          142 t
ORIGIN      
        1 ttacatcatc attgtaaata attaaattaa cttccataac attaaaatat gtatccactg
       61 acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac
      121 cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga
      181 aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc
      241 ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa
      301 gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct
      361 ccactttcac agaacctaac ggcacttcgc tgtcggatgc ttttgctctt tggaattatc
      421 aaagcggcag atattctttc atcttaaatt ttacgtctt
//
LOCUS       DAZFRJ010000651          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000651 DAZFRJ010000000
VERSION     DAZFRJ010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.657"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>457)
                     /locus_tag="ACHKUN_005406"
     CDS             complement(<1..>457)
                     /locus_tag="ACHKUN_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6663434.1"
                     /translation="IADKEFLINLIVRLEQGLSPSLEQENIVQLISVSSVISSLSSLC
                     DWLERKRVIILLDDAAISLTPEYLYEFFDIVRSLKTSKIALKASVYPGTTEYGPRFHV
                     AHDAETIDAWISVQHPNYSSVMDAIAQARFPGYDGIPDDLKELFKFSAFG"
BASE COUNT          150 a           95 c           80 g          132 t
ORIGIN      
        1 accaaatgct gagaatttaa ataactcttt caagtcatcc ggaatgccat catacccagg
       61 aaaccttgcc tgcgctatag catccatcac tgaggaataa ttagggtgct gtaccgaaat
      121 ccaagcatca attgtttcag catcatgagc aacatgaaac cttgggccat attcggttgt
      181 tccaggataa accgatgcct ttaaagctat ttttgaggtt tttagactac gtacaatatc
      241 aaaaaactcg taaagatatt caggagttaa gcttattgct gcatcgtcta ataaaataat
      301 gactctttta cgttcaagcc aatcacatag tgaactcaac gaggatatga cagaagaaac
      361 actgatcaat tgaacaatgt tttcttgttc taatgatggc gaaagcccct gctcaagcct
      421 tacgattaaa ttaattagga actctttatc cgctatg
//
LOCUS       DAZFRJ010000652          454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000652 DAZFRJ010000000
VERSION     DAZFRJ010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.658"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..411
                     /locus_tag="ACHKUN_005407"
     CDS             <1..411
                     /locus_tag="ACHKUN_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO6663435.1"
                     /translation="NKKNRGFEGLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQ
                     VTIPEELNDIHVKDISGLDFNNESLMILSDESRKLLKFNLTEMSFVEMMDLTKGNHSL
                     TSDLLQPEGIVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          143 a           89 c           97 g          125 t
ORIGIN      
        1 aataaaaaaa atagagggtt tgaaggactg gcatggaaag cagaaggccg gatgctgttt
       61 gtcgctaaag aacgacgccc tacaggcatg tttgcctatc agctttctcc agatctgctg
      121 agtgcaaaac aagtaacgat tccagaggaa ctaaatgata tacatgttaa agatatatcc
      181 ggcctggact ttaataatga aagtctgatg attctgtccg atgaatcgcg taagttactc
      241 aagtttaacc tgacagaaat gtctttcgtt gagatgatgg acctgacaaa aggaaatcat
      301 tctctgacca gtgatttgct ccaacctgaa ggcattgtaa ctctgtctga tgaaagcatt
      361 tatgtcgcta gtgaacctga tatcctcgct aaatttgtcc ctaataaata agtctgtcac
      421 tagccttagt attgagcgac caaatctggc aatc
//
LOCUS       DAZFRJ010000653          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000653 DAZFRJ010000000
VERSION     DAZFRJ010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.659"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>445
                     /locus_tag="ACHKUN_005408"
     CDS             <1..>445
                     /locus_tag="ACHKUN_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6663436.1"
                     /translation="QNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLD
                     YVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNE
                     KIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYK"
BASE COUNT          165 a           82 c           79 g          119 t
ORIGIN      
        1 caaaataaaa gtgatataca gacgctaaaa acagaatgtt attgccttct agcggaatgc
       61 catatgtctt tggcacttca tggaaaaagt gaacttgaac ttgctgctca aaaagcatta
      121 gagcttttag attatgtatc agacataacc actgtcgatg gaaaaatttt agctattatg
      181 ggactgataa ctggtctgtc tggacaagca aaagtatctc atatcttatt tgaacaggct
      241 aagatacacc caactgatat agcctctctc tactactata gggcactagt ccactttcat
      301 aatgaaaaaa ttgaagaggc aaggatttgt atagacaaat cactacaact cgaacccaga
      361 agacgaaaag cagttgtgat aaaagaatgt gtagatatgt atgtgcctaa cccgctcaaa
      421 aacaacatta aactctacta taaag
//
LOCUS       DAZFRJ010000654          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000654 DAZFRJ010000000
VERSION     DAZFRJ010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.660"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..94)
                     /locus_tag="ACHKUN_005409"
     CDS             complement(<1..94)
                     /locus_tag="ACHKUN_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663437.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEE"
BASE COUNT          127 a          100 c           93 g          117 t
ORIGIN      
        1 cctcctcagc gaggttgcct gaataccctg tcgtgcccat ttttccatcg gacgcagttg
       61 tctgctctct cgaactgctg gcaataaaaa tcaaccagac agcttgatgt ccgttacatg
      121 caggactaat cacgattggt taccgtttac aaaacttgcg taacattgga ggagaacgaa
      181 taatattctc tagctggtac gcgttccata gcgaaacggg caaggtccaa cagcaagagc
      241 tgaaactcag ggggatttat cagtcgattt attcgccatg gatgaatcac gctgtacggt
      301 tatggtagct cttagcgaca aggcataatc atcagtacac caatgtaaca accgttgaaa
      361 aagactcatt attttatgaa ccagccatcc catttccaga gtggtaaata tgatttgtta
      421 aagtgtaaca gtactct
//
LOCUS       DAZFRJ010000655          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000655 DAZFRJ010000000
VERSION     DAZFRJ010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.661"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..117)
                     /locus_tag="ACHKUN_005410"
     CDS             complement(<1..117)
                     /locus_tag="ACHKUN_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663438.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAE"
     gene            complement(373..>437)
                     /locus_tag="ACHKUN_005411"
     CDS             complement(373..>437)
                     /locus_tag="ACHKUN_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO6663439.1"
                     /translation="INVNFFSSDRTSFCECNRFP"
BASE COUNT          125 a           91 c           87 g          134 t
ORIGIN      
        1 ttctgcaaac tttcttttta ccgtccaata atcaatattt ctattgtgat tgttaaccac
       61 tgcgtgcatt ggcgggattg aagggtgaaa aatatgtgca attgggaagc ctttcattga
      121 tgcctgcctt gtcgtcatga caaacaataa tagatgctta ttttcctcgc agggttcttt
      181 cgcactatca aaaaacgctc atttacccgg cgttacagta aacgaagcaa cattctgtgg
      241 cctacgacga tcaaaagctc tgtgaaaatt tcccttgttg tctttgtctg accttatcgc
      301 ggcgaccagc aattattcgt tttgtgatcc tcaacacaaa atgctattcg tcgcctatga
      361 ggggagaaga atttatggaa aacgattgca ttcacaaaat gacgtcctgt cagaagagaa
      421 aaagttgacg ttaatca
//
LOCUS       DAZFRJ010000656          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000656 DAZFRJ010000000
VERSION     DAZFRJ010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.662"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..413
                     /gene="ler"
                     /locus_tag="ACHKUN_005412"
     CDS             42..413
                     /gene="ler"
                     /locus_tag="ACHKUN_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO6663440.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
BASE COUNT          157 a           73 c           94 g          104 t
ORIGIN      
        1 ctaatagctt aatatattaa agcatgcgga gattatttat tatgaatatg gaaactaatt
       61 cgcacacaac aagcccatac attcagctta ttgagcaaat tgaagtgtta caacaggaag
      121 caaagcgact gcgagagcag gaaattcaaa gtgtaattga gtcgattcaa aagcagatta
      181 cttattacaa tataacccta caagagctgg gatatactaa tgtgcctgat gatggccttg
      241 ctcgccggaa ctcatcgaaa ggagtttatt atcgcaatga agaagggcag acctggtcgg
      301 gagttggccg acagccacgc tggcttaaag aagcactgtt gaatggaatg aagaaagaag
      361 attttcttgt gaaggacacc gaagacgaaa taataccgct gaaaaatatt taacatgaaa
      421 tcattaaa
//
LOCUS       DAZFRJ010000657          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000657 DAZFRJ010000000
VERSION     DAZFRJ010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.663"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>426
                     /gene="nleB"
                     /locus_tag="ACHKUN_005413"
     CDS             <1..>426
                     /gene="nleB"
                     /locus_tag="ACHKUN_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO6663441.1"
                     /translation="LLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAG
                     TLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENG
                     AIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRH"
BASE COUNT          134 a           63 c          101 g          128 t
ORIGIN      
        1 atctgttagg cttattaaga gaagagttaa aaaatatccc agaaggtaag gactctttga
       61 ttgagtcata tgcagaaaaa agagaacata cttggtttga ttttttcagg aatttggcca
      121 tgttgaaggc tggaactttg tttacagaga cgggaaaaac tggatgccat aacatatcgc
      181 cctgtagcgg atgtatatat cttgatgccg acatgattat taccgataaa ttaggagtcc
      241 tgtatgctcc tgatggtatc gctgtgcatg tagattgtaa tgatgagata aaaagtcttg
      301 aaaatggtgc gatagttgtc aatcgtagta atcatccagc attacttgca ggcctcgata
      361 ttatgaagag taaagttgac gctcatccat attatgatgg tctaggaaag ggtatcaagc
      421 ggcatt
//
LOCUS       DAZFRJ010000658          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000658 DAZFRJ010000000
VERSION     DAZFRJ010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.664"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..415
                     /locus_tag="ACHKUN_005414"
     CDS             <1..415
                     /locus_tag="ACHKUN_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO6663442.1"
                     /translation="LAARKARAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWD
                     ALNVYCSNGWVEIDNNIAENALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNN
                     VEPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSSQ"
BASE COUNT          115 a          100 c          125 g           84 t
ORIGIN      
        1 tctggcggca agaaaagcca gagccgcgcc actgatgcag tcactgtatg actggataca
       61 gcaacagatg aaaacactgt cgcgtcactc agatacggca aaagcgttcg catacctgct
      121 gaaacagtgg gatgcactga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa
      181 catcgcagag aacgccttac ggggagtggc cgtaggccgg aaaaactgga tgttcgcggg
      241 ttccgacagc ggtggtgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct
      301 gaacaatgtg gagccagaaa agtggctgcg ttacgtcatt gaacatatcc aggactggcc
      361 ggcaaaccgg gtacgcgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc
      421 aata
//
LOCUS       DAZFRJ010000659          423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000659 DAZFRJ010000000
VERSION     DAZFRJ010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.665"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>423
                     /locus_tag="ACHKUN_005415"
     CDS             <1..>423
                     /locus_tag="ACHKUN_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663443.1"
                     /translation="LEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQ
                     NTELAKLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAAR
                     ASNEAELLGYGQGERARERMRELQQIRDSFRQKDADLQS"
BASE COUNT          129 a          100 c          135 g           59 t
ORIGIN      
        1 ctggaagcct ggcgtaataa cgaggcgaac aaacctaaat cccggggtgg aaaatcagag
       61 acggaaaaag cggaagacag tttttcccgg ctactgaagc agcagaaaga gcaactggca
      121 ctggcgggtc agaatacaga gctggcgaag ctgaagtacc agactgcgca gggcgaactg
      181 aaaaccctga cggagatgca gaagcaggaa ctgctgcgta acgcggccct gattgaccag
      241 caaaaaatcc gggaacagtt gcgatcccgg gaagagaccc tgaagaatga taatgtggct
      301 gcgcgtgcat caaatgaagc cgaactgctg ggatacgggc agggagaacg agccagggaa
      361 cgcatgcggg agttgcagca gatccgcgac agcttccgcc agaaggatgc ggaccttcag
      421 tct
//
LOCUS       DAZFRJ010000660          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000660 DAZFRJ010000000
VERSION     DAZFRJ010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.666"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>420
                     /locus_tag="ACHKUN_005416"
     CDS             <1..>420
                     /locus_tag="ACHKUN_005416"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6663444.1"
                     /translation="IKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHS
                     NYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYT
                     GLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITV"
BASE COUNT           92 a          117 c          130 g           81 t
ORIGIN      
        1 cataaaaccg gtatgggcgc ttgccaacga catgaactgc agtgcaggtc agttgcttgc
       61 cagtgccgcc tcccggcgtc tggtcacgca gaccgcccgg acaggctcca tcggcgtcat
      121 gatggctcac agtaattacg gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat
      181 ttacagcggc agccataagg tggatggcaa cccctacagc catcttccgg atgacgtccg
      241 ggagacactg cagtaccgga tggacgcaac ccgccagatg tttgcgcaga aggtgtcggc
      301 atataccggc ctgtccgtgc aggctgtgct ggataccgag gctgcagtgt acagcggtca
      361 ggaggccatt gatgccggac tggctgatga acttgttaac agcaccgatg cgatcaccgt
//
LOCUS       DAZFRJ010000661          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000661 DAZFRJ010000000
VERSION     DAZFRJ010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.667"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..137)
                     /locus_tag="ACHKUN_005417"
     CDS             complement(<1..137)
                     /locus_tag="ACHKUN_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6663445.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SG"
BASE COUNT          123 a           79 c          106 g          112 t
ORIGIN      
        1 ccgcttttac ccataatgcc aatcacgggt tcgtgactgg tgagtttgcg cagatgttcc
       61 aggatgtgac gggaaagtga gtaaggcagg gaggagagcg gtttttcaat tgcctcaatg
      121 gcatcagacg gattcataca tgtattcccg gtgaaaaaag acaaaacccc gcgcaccgga
      181 aaggtggcgg gggattcaca acgataatat ctgtctgaaa ttatttggaa tgcaggtata
      241 tgtggctgat ttgtatcagc taatgagtac aggtctgaat ctaatctgac agtccgctct
      301 gtgccagaag cgggcgttag gttgagtaaa gaaaagccca taaaattccc tgcgtttgat
      361 aaacccggat atgaaaaatt attactttca gagtatgatt atcttgctta aattaaccta
//
LOCUS       DAZFRJ010000662          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000662 DAZFRJ010000000
VERSION     DAZFRJ010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.668"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..155
                     /gene="kil"
                     /locus_tag="ACHKUN_005418"
     CDS             <1..155
                     /gene="kil"
                     /locus_tag="ACHKUN_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6663446.1"
                     /translation="HEGRTYIASANNIKKRKLYIRNLTTKTCISDCMIKVFLGRDGLP
                     VKAESW"
     gene            155..325
                     /locus_tag="ACHKUN_005419"
     CDS             155..325
                     /locus_tag="ACHKUN_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO6663447.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
BASE COUNT          156 a           74 c           82 g          105 t
ORIGIN      
        1 ttcatgaagg ccggacatat atcgcctcgg caaacaatat taaaaagcga aaactttata
       61 ttcgtaacct gactacaaaa acatgcattt ctgactgcat gattaaagtc ttcctcggtc
      121 gtgatggttt acctgtaaag gcggagtcat ggtgatgact aagaaaataa aatgtgctta
      181 ccacctttgc aataaaggaa ttgaagaaag caaaagcatt aaaagaccac ttcatttcat
      241 gcgtggagtt atcccaacga cggaaatgaa aaaatattgt agtgaaaatt gtgccgaaaa
      301 agaccagatg gcacacgaac tttaattaac tgactatgcg aaactgaatt tatgccagca
      361 atggcaggga ttcgctcaac cttaattaag gagaaaaaat gattaccaat tatgaag
//
LOCUS       DAZFRJ010000663          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000663 DAZFRJ010000000
VERSION     DAZFRJ010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.669"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..206
                     /locus_tag="ACHKUN_005420"
     CDS             <1..206
                     /locus_tag="ACHKUN_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFO6663448.1"
                     /translation="LVLLIVNNKMLNLPIRKLFPTRIASSYGMNFEFMPELKWGFGYP
                     GAIIFMILAGLTPYLYFKRKNWL"
     gene            complement(231..>414)
                     /locus_tag="ACHKUN_005421"
     CDS             complement(231..>414)
                     /locus_tag="ACHKUN_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663449.1"
                     /translation="CKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTT
                     IMLSAIPSAKMSLSAH"
BASE COUNT          117 a           85 c           91 g          121 t
ORIGIN      
        1 gattggtctt gctcattgtt aataacaaaa tgttgaattt accgattagg aaactttttc
       61 ctacgcggat tgcctccagc tatggcatga actttgagtt tatgccagaa ctgaagtggg
      121 gcttcggcta ccctggcgcg attatcttta tgatcctcgc gggcctgaca ccgtatctgt
      181 actttaagcg taagaactgg ctgtaactat acgatgtcgc tgtgagtaag ttagtgtgct
      241 gacagcgaca ttttagccga gggaatcgca gataacataa ttgttgtgaa taatatccat
      301 gccacgttga gttggacgct taatgactca catctcaccc cttcaattgc tgcagagata
      361 atagaaaaaa gtgtcaagcc caatgaaatc aatacaaagt agattgcctt acat
//
LOCUS       DAZFRJ010000664          411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000664 DAZFRJ010000000
VERSION     DAZFRJ010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.670"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..191)
                     /locus_tag="ACHKUN_005422"
                     /pseudo
     CDS             complement(77..191)
                     /locus_tag="ACHKUN_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          112 a           84 c           97 g          118 t
ORIGIN      
        1 taccatcaaa attcatcatc tttgccaaag aaaaatgttc agaaaataat ccatggaaaa
       61 ttgtccggag cgcttactat tttaatggat tgttagtctt tgcatgagca agcggactga
      121 tacatttctc tttgttctca ttcagaaaat ctcatcagtc ggcgttcaga ccggtgttgt
      181 gctttatgga tgcgttacgc tcctgatgac gtcatttgac gttcaacaac atcacggggc
      241 cgcacgacat ttcacgtcag ttagtgctat cgctcaggaa caaattttcc cgaattggga
      301 tatgcccgca aattgctggt gatgtgggga gaatctggtt gagttcggga gaattgattt
      361 ggaggcagaa cgcttaaatt gtggcgtcca gaaacgaaaa acggacctcc g
//
LOCUS       DAZFRJ010000665          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000665 DAZFRJ010000000
VERSION     DAZFRJ010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.671"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>410
                     /locus_tag="ACHKUN_005423"
     CDS             <1..>410
                     /locus_tag="ACHKUN_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFO6663450.1"
                     /translation="NGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSK
                     ITPVTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTIT
                     VNGVATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
BASE COUNT          108 a           96 c          131 g           75 t
ORIGIN      
        1 aatggcacgg tggatgtgtt ccgtggctgg gtcagcagta tcggtaaggc cgtgacggcg
       61 aaagaagtga tcacccgcac ggtgaaagtg accaacgtgg gcaaaccttc tgtagcggaa
      121 gaacgcagca aaattacgcc ggtcactgcg attaaggtga cgccgacatc cggtacggtg
      181 gcaaaaggga aaacaaccac cctgacggtt tcttttgagc cggaaagtgc aaccgacaag
      241 acgttcagag cggtttccgc cgatccgtcg aaagccacca ttagtgtgaa agatatgaca
      301 attacggtaa acggcgtggc gacaggtaag gtgcagatcc ctgtggtgag cggaaatggt
      361 cagttcgccg cagtggctga agtcaccgtt actgaagcgg gcgctgcagg
//
LOCUS       DAZFRJ010000666          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000666 DAZFRJ010000000
VERSION     DAZFRJ010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.672"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..324
                     /locus_tag="ACHKUN_005424"
     CDS             <1..324
                     /locus_tag="ACHKUN_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663451.1"
                     /translation="MVSIRLPFISIYWLNREATNYWYTCARAAFNDPDWFVKNHHAVR
                     QAKRKANMTYMKAYQKAWKEHRDRYQQDMEKLESENMELRRKLGEAKRDIDAYKRLFN
                     GESHA"
     gene            284..>406
                     /locus_tag="ACHKUN_005425"
     CDS             284..>406
                     /locus_tag="ACHKUN_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO6663452.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHV"
BASE COUNT          140 a           86 c           94 g           86 t
ORIGIN      
        1 atggtgtcta tccggttgcc atttatcagt atttattggc taaacaggga ggcaacaaat
       61 tattggtata catgcgccag agcagcattt aacgaccctg actggtttgt gaaaaaccac
      121 cacgcagttc gtcaggcaaa gagaaaggcc aatatgacat acatgaaggc gtatcaaaaa
      181 gcatggaaag aacaccgcga tcgataccaa caagacatgg aaaagcttga atcagaaaac
      241 atggaattaa gacgaaagct cggtgaagca aaacgagaca ttgatgctta caagcgactt
      301 tttaatggtg aaagccatgc ttagcccatc ccaatccctt caataccaga aagaaagcgt
      361 cgagcgagct ttaacgtgcg ctaactgcgg tcagaagctg catgtg
//
LOCUS       DAZFRJ010000667          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000667 DAZFRJ010000000
VERSION     DAZFRJ010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.673"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>401)
                     /locus_tag="ACHKUN_005426"
     CDS             complement(<1..>401)
                     /locus_tag="ACHKUN_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6663453.1"
                     /translation="GSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKE
                     TPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSG
                     EEIEPPDSVTFHIWTAYSPFTTWVQIVKDWM"
BASE COUNT           95 a          110 c          109 g           87 t
ORIGIN      
        1 ttcatccagt ctttgacaat ctgcacccag gtggtgaacg ggctgtacgc cgtccagatg
       61 tgaaaggtca cactgtcagg cggttcaatc tcttcaccgg atgacgaaaa ccagagaatg
      121 ccatcacggg tccagatccc ggtcttttcg cagatataac gggcatcagt aaagtccagc
      181 tcctgctggc ggatgacgca ggcattatgc tcgcagagat aaaacacgct ggaggggtca
      241 tccggcgtcc atttgaggcc aaacggcgtc tctttgtcgc caaatttaag atactgctcc
      301 tccccgcagt gcgggcaggc aacatgaaaa cgcataaaat gcggggattc actggctgca
      361 cgctcaatct ggcaggtgcc tctcactttg ggcgtggagc c
//
LOCUS       DAZFRJ010000668          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000668 DAZFRJ010000000
VERSION     DAZFRJ010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.674"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          125 a           66 c           89 g          120 t
ORIGIN      
        1 tttattctta gaatggattg taaaagatgg ttatttagag gaaataattt tgtctggttc
       61 ataaattcat cttctttgtt gagaagatta actgcctagg tgaaattatc ctctatgacc
      121 gggacgctgc aggccacctg gcagaagtca tcacgcagga agacaagtcg ctgtatccct
      181 ttgaaaaatc cggcaggcta atgggcatct tcagcccagg cggcacatca cagcgaattt
      241 gaaggcaaat gcgaaaatta gtgatcttga agttcataca gtattggtaa aaacaggcga
      301 agcttttcca atgtgtaaga attttcaaat ttcaatattt ggcgctgttg ttacaagcgg
      361 gtgattatat gtgtgaatta attaatgaag catcaaaata
//
LOCUS       DAZFRJ010000669          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000669 DAZFRJ010000000
VERSION     DAZFRJ010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.675"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>399
                     /locus_tag="ACHKUN_005427"
     rRNA            <1..>399
                     /locus_tag="ACHKUN_005427"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          108 a           94 c          122 g           75 t
ORIGIN      
        1 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
       61 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      121 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      181 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      241 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      301 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      361 cggcctgcgc ggaagatgta acggggctaa accatgcac
//
LOCUS       DAZFRJ010000670          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000670 DAZFRJ010000000
VERSION     DAZFRJ010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.676"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>396
                     /locus_tag="ACHKUN_005428"
     CDS             <1..>396
                     /locus_tag="ACHKUN_005428"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO6663454.1"
                     /translation="ALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKACIAKSFLQ
                     HEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWSEQFRYDN
                     NISKAMAEQLFDAVGDQNSGVLKWVKAHW"
BASE COUNT          123 a           64 c           84 g          125 t
ORIGIN      
        1 aagcattatc tgacactagg cttaatgagg ccaaatgctt gcttgatcat ggcttttttc
       61 atggtgcata ttatctttgt gggtatgcag ttgaatgtgc attgaaggcc tgtattgcca
      121 agtcattttt acaacatgag tttccaaaca aaaaagtcgt aaatgattca tatactcatg
      181 atttgtcgca acttctcaaa attgccaact tacatcaaat tttgattgct gacgcaaaaa
      241 atgatgtttc gttggagatt aactggtcgg tcgttaaaga ctggagcgag caatttagat
      301 acgacaataa cataagtaaa gctatggccg aacaattgtt tgatgctgta ggtgaccaaa
      361 attctggagt tttgaaatgg gtaaaagcac actggt
//
LOCUS       DAZFRJ010000671          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000671 DAZFRJ010000000
VERSION     DAZFRJ010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.677"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..352
                     /locus_tag="ACHKUN_005429"
     CDS             <1..352
                     /locus_tag="ACHKUN_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6663455.1"
                     /translation="IVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQ
                     MLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRD
                     EGGDGKVVVIGRQA"
BASE COUNT           85 a           98 c          130 g           83 t
ORIGIN      
        1 catcgtgggg tcgttcttca cggccggggc atcaatggcg ttatggggtt cagccctggc
       61 agccggtggt ttttctgcca ccacgatgct gttttctctg ggggccagca tgattctggg
      121 tggtgtggct cagatgctgg ccccgaaggc taaagtaccg gagtacaaaa gcacggataa
      181 cggcagacag aacacgtact tttcctcgct ggataacatg attgcccagg ggaacccgat
      241 gccggtgcct tacggtgaaa tgctggttgg ctcccgccgt atatcccagg acatcagtac
      301 ccgtgatgaa ggcggtgacg ggaaggtggt ggttatcggg cggcaggcat aaaagcgaaa
      361 aaatcccgca gtgctcacgg acaggaactg cgggag
//
LOCUS       DAZFRJ010000672          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000672 DAZFRJ010000000
VERSION     DAZFRJ010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.678"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..189)
                     /locus_tag="ACHKUN_005430"
     CDS             complement(<1..189)
                     /locus_tag="ACHKUN_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO6663456.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKIL"
BASE COUNT          137 a           53 c           74 g          128 t
ORIGIN      
        1 tagtatttta tcaatttttg aagattcatt attgaataaa taacaataaa aaaacaggac
       61 ctcagattga ggtgcacact ccagtatcca ttcatgacca tcgtgatcat ggaaagatag
      121 ggcgttagtt tgttcttcaa gagttattcc gaacgtatgt gcaagatctg tgcgaaattg
      181 ttcattcata ttgtagtcct gtcattcttt caatgagggt atgcttaaac gtaattaact
      241 cgtgagctct ggcacaaata ataagacgaa aatataagat gcaagtaata gtgttgcata
      301 tttatgatag catgtgataa atgtagtgca actacttgta gatagtgccg gaggaaaatt
      361 taaatataaa acaattattg tttttaatga aa
//
LOCUS       DAZFRJ010000673          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000673 DAZFRJ010000000
VERSION     DAZFRJ010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.679"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..>391
                     /locus_tag="ACHKUN_005431"
     CDS             9..>391
                     /locus_tag="ACHKUN_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO6663457.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTYPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPG"
BASE COUNT          130 a           57 c           81 g          123 t
ORIGIN      
        1 tggtattcat ggaaaataat gataaattct tatcacaaga cttattggaa tcttatgcca
       61 ttcgtttgtt gagcggacca ttgaatggat gcgagtatga aatacttaat gggcgtctac
      121 ttgttatcat tggtaatgat gtttcgttag gaagaagtga tgctttttct gagttaccag
      181 aaaacacaat agtcgttcct tatggcgagc ttacaggtag ttttgagata ataattacta
      241 cctaccctga tctggtagta acattcagag aattaacagc acaagaacct gaagatagaa
      301 cgttaacatt gaatcagcaa atagaagttt taggtctcaa atttgctgta aaagaaaaaa
      361 atgaagtttg gcagtattca ttgccaggca t
//
LOCUS       DAZFRJ010000674          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000674 DAZFRJ010000000
VERSION     DAZFRJ010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.680"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>389)
                     /locus_tag="ACHKUN_005432"
     CDS             complement(<1..>389)
                     /locus_tag="ACHKUN_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663458.1"
                     /translation="FSWKALGWALIYFWYFSTLLQIIICLTGYSGTNGLRDSLLFSSL
                     WLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLFVMFESNTSEAS
                     EYFSQYFSLKILFISTIYTAIAILLWTR"
BASE COUNT          143 a           76 c           76 g           94 t
ORIGIN      
        1 cgagtccata gcagaatagc aatagctgta tatattgtcg atataaatag tatttttaaa
       61 ctaaaatact gactaaaata ttcgctggct tcacttgtat tactttcaaa catgacgaac
      121 agtacactct gagagaattc ctgcccgtag attagataat agcaaagggc agcaagagat
      181 gtgccccata aaaccacgcc gattaatgct gctgtaacct ttattctctc tgggaaaaga
      241 aaaacaggga ttaaccagag agagctgaaa agtagcgagt cacgaagtcc attggtgcca
      301 ctatatccag tcagacaaat gataatctga agtaaagtgg aaaaatacca gaaataaatg
      361 agagcccatc ccagggcttt ccagctaaa
//
LOCUS       DAZFRJ010000675          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000675 DAZFRJ010000000
VERSION     DAZFRJ010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.681"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..>382
                     /locus_tag="ACHKUN_005433"
     CDS             13..>382
                     /locus_tag="ACHKUN_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepL/TyeA/HrpJ family type III secretion system
                     gatekeeper"
                     /protein_id="HFO6663459.1"
                     /translation="MANGIEFNQSTASVFNSNSLDFELESQQLTQKNSSNSYSPLLNL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKE
                     LSLKNSGLLEQHDPNLAMFGN"
BASE COUNT          132 a           60 c           77 g          113 t
ORIGIN      
        1 cgtgagtttc caatggctaa tggtattgaa tttaatcaaa gcaccgcatc tgtttttaat
       61 tctaattcat tagattttga attggaatct caacaattaa cgcaaaaaaa ttcttctaat
      121 agttattctc cattattaaa tttacaaaat gaattagcga tgattactag ttcatcgctt
      181 tctgaaacga ttgaagggct aagcctggga tatcgcaaag gcagtgcaag gaaagaagag
      241 gaaggtacga caacggagaa gctgcttaat gatatgcaag agctgcttac tctgaccgat
      301 agcgataaat taaaagaact atcattaaag aacagtgggc tgctggaaca gcatgatcct
      361 aatttggcta tgtttggtaa ta
//
LOCUS       DAZFRJ010000676          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000676 DAZFRJ010000000
VERSION     DAZFRJ010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.682"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>381)
                     /locus_tag="ACHKUN_005434"
     CDS             complement(<1..>381)
                     /locus_tag="ACHKUN_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6663460.1"
                     /translation="LSEADRRLQVQSDLPWWLVCRGEIHKFRCVPHLTGRRFEHGVTD
                     CYTLFRDAYHLAEIEMPDFVREDDWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVLLC
                     CFGSSVPNHAAIYCGDGELLHHIP"
BASE COUNT           98 a          134 c           89 g           60 t
ORIGIN      
        1 caggaatatg gtgcagcagc tcgccgtcac cacagtaaat agcggcgtga ttcggcaccg
       61 atgaaccaaa acagcacagc agcacatcgc ccggctgcgc cgctgacaac ggcacctgat
      121 acagccctgt ggcctccaga ttatccagat agagattctg accgttacgc caccagtcat
      181 cctcacgcac aaaatccggc atctcaatct ccgccagatg ataagcatcc cggaacagtg
      241 tgtaacagtc cgtcacaccg tgctcaaagc gccgcccggt gagatgcggc acacagcgga
      301 acttatgaat ctcaccccgg cagaccagcc accacggcaa atcactctgc acctgcagcc
      361 gccggtcggc ctcactcagc c
//
LOCUS       DAZFRJ010000677          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000677 DAZFRJ010000000
VERSION     DAZFRJ010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.683"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..295)
                     /locus_tag="ACHKUN_005435"
     CDS             complement(<1..295)
                     /locus_tag="ACHKUN_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO6663461.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELA"
BASE COUNT          133 a           55 c           71 g          118 t
ORIGIN      
        1 ttgctagttc tttcatttct gcaaataaaa tatcagtgca ttcacgttgt gatatttttg
       61 ttccggaact tgaggcacta aaagcaacat taatagaacc tgggccaggg aaatggtatt
      121 taataagtct gtaaggatca acttttgcag agatatcgct taggccgagt gagagaaagt
      181 cttgttctac catatcacca tattgcatat catcagcaga aaaatcatcg aataaactaa
      241 tagtttcgaa aagtgtacag ggaatattaa ttggcggaag taatatgtta ttcatttgca
      301 tttgccttgg ttatatttaa gctcaatatc aattcgagaa caaatgctag ggtaaaaaaa
      361 tatttaaaaa agaagta
//
LOCUS       DAZFRJ010000678          373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000678 DAZFRJ010000000
VERSION     DAZFRJ010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.684"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHKUN_005436"
     CDS             <1..198
                     /locus_tag="ACHKUN_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO6663462.1"
                     /translation="LWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEET
                     TRPTLKELAAKLSGGVNGYSR"
     gene            182..>373
                     /locus_tag="ACHKUN_005437"
     CDS             182..>373
                     /locus_tag="ACHKUN_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO6663463.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDDLN
                     RAINDAESVLGTTSRRRRPL"
BASE COUNT           89 a           91 c          120 g           73 t
ORIGIN      
        1 ctgtgggata acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta tgcctacgcg
       61 gcattacgtg tgtccgtgca acgctggcag cttgatctgg ctgtactggc aaaatcccgg
      121 gaagaagaga cgacccggcc aaccctgaaa gaactggcag cgaagctgtc cggaggagtg
      181 aatggttaca gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt tattaaccgg
      241 taaacgggtg gtgtctgtcc agaaagatgg acgaagaatt gaatatacgg cggcctctct
      301 ggatgatctt aaccgggcga tcaatgatgc ggagtcagtg ctggggacaa caagccgccg
      361 ccgtcgtccg ctg
//
LOCUS       DAZFRJ010000679          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000679 DAZFRJ010000000
VERSION     DAZFRJ010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.685"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..183
                     /locus_tag="ACHKUN_005438"
                     /pseudo
     CDS             1..183
                     /locus_tag="ACHKUN_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
BASE COUNT          108 a           90 c           73 g           98 t
ORIGIN      
        1 ctacagcaag aaatgccgtg acgtgccagt aaacgttatg acgatgtaga agtgatcatc
       61 aaatccacca gcaacgatgg cctttcagtt actctcaccg ccgataaaga ttctgcaaaa
      121 acttttgttc aggagacgct gaaagatacc tgggagtctg ctgacgagtg gtttgttcgc
      181 taattaacga gtaaaatcag taacggctgg aaatcattca atactcacac tatctaaaag
      241 ttcaccagcc aacagcggta agttctttca tacgacgtgc cgcggatcct attattctag
      301 tatgaaatcc ctaaccgatt tcaaattctc cagactgctc agattctctt ttatcccacg
      361 ctggcgctg
//
LOCUS       DAZFRJ010000680          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000680 DAZFRJ010000000
VERSION     DAZFRJ010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.686"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..324
                     /locus_tag="ACHKUN_005439"
     CDS             <1..324
                     /locus_tag="ACHKUN_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO6663464.1"
                     /translation="VYINENNESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGY
                     LDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFG
                     VKSEK"
BASE COUNT          128 a           50 c           84 g          103 t
ORIGIN      
        1 gtgtatatta atgaaaataa tgagagctac ctgaaagctc agtttgaaaa attatctcaa
       61 cgtgatatga agaagtacct gggagagatg aagcgtctgg ctgatggggg agaaatgaat
      121 tttgatggct atctggataa gatgtcacat ctggtgaatg aaggaacgct cgatcctgat
      181 attttaagca aaatgcgaga tgctgcacca caattaatta gcttcgcgaa gtcgtttgac
      241 ccaacctcaa aggaagagat taaaatactt acagacactt ctaaattaat ttatgatttg
      301 ttcggggtta aatcggagaa ataatatgtg aagttcttcg atagtatgga aggcattata
      361 taaaa
//
LOCUS       DAZFRJ010000681          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000681 DAZFRJ010000000
VERSION     DAZFRJ010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.687"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>362
                     /locus_tag="ACHKUN_005440"
     CDS             <1..>362
                     /locus_tag="ACHKUN_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO6663465.1"
                     /translation="KIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKL"
BASE COUNT          109 a           70 c           80 g          103 t
ORIGIN      
        1 taaaatcgtt gtttatcatc aggaacaagt aaatggaccg cttgtcgacg agtcagggcg
       61 ggttattttt gccgatttta acccatcgaa acgaccgtgg tcggtggctt cagatgactc
      121 taacaacagc tggaatccgg catacaattg ctttgatcgt ccgggtaaaa aatgtatctc
      181 ttttacgcta cgtatcaacg gcaaagatca cgatttgtta gcggtagata aaattcatgt
      241 cgatttaaac tggcggtatc tgaacgagta tcttgatcaa atcagcgcta atgatgaagt
      301 tctatttttg aaacaaggtc atgagatcat tgccaagaat caactcgctc gtgaaaaact
      361 ga
//
LOCUS       DAZFRJ010000682          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000682 DAZFRJ010000000
VERSION     DAZFRJ010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.688"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..>360
                     /locus_tag="ACHKUN_005441"
     CDS             169..>360
                     /locus_tag="ACHKUN_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6663466.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           90 a           89 c           89 g           92 t
ORIGIN      
        1 aaaatcggcc agttttctct gcctgcagtc cgcatgccgt atcgggcctt gggttctaac
       61 ctgttgcgta gatttatgca gcggactgcc tttctcccaa agtgataaac cggacagtat
      121 catggaccgg ttttcccggt aatccgtatt tgcaaggttg gtttcactat ggaacatgaa
      181 cttcattata tcggtatcga caccgctaaa gagaaactgg atgtcgatgt gttgcgtcct
      241 gatggtcgtc atcgcaccaa aaaattcgct aacaccacta aagggcacga tgagctggtg
      301 agctggctga aaggtcacaa gattgaccat gcgcatatct gcatcgaagc gaccggcacc
//
LOCUS       DAZFRJ010000683          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000683 DAZFRJ010000000
VERSION     DAZFRJ010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.689"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..197
                     /locus_tag="ACHKUN_005442"
     tRNA            122..197
                     /locus_tag="ACHKUN_005442"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT          102 a           82 c           83 g           93 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaacga aagttgttcg tgagtctctc aaattttcgc aactctgaag
//
LOCUS       DAZFRJ010000684          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000684 DAZFRJ010000000
VERSION     DAZFRJ010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.690"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>359
                     /locus_tag="ACHKUN_005443"
     CDS             <1..>359
                     /locus_tag="ACHKUN_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663467.1"
                     /translation="GHQLTNAMTVASVVKPYDFSNLGAINSIDKSVSDIPALHTIVEQ
                     AKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSKGKCLGLSLLYLDT
                     GGYGSRYQKLRHNIETA"
BASE COUNT          121 a           59 c           68 g          111 t
ORIGIN      
        1 tggacatcag ttaacaaatg ctatgacagt tgcttcagtg gttaagccat atgactttag
       61 taatcttggt gccataaata gtatagataa gtccgtttct gatatacctg cattgcatac
      121 aattgttgaa caagcgaagt ataatttact ttcatggaat gatttttata atactcatgc
      181 gtcaatatgg gatacaatag caaggcagca taaaagtaca aatatcgaat ttcacccaca
      241 aagtcttctt tttgatagag attcgaaagg aaagtgcctt ggtttgtctc tcctgtatct
      301 tgatacagga ggttatggaa gcagatatca aaaactgaga cacaatattg aaactgcca
//
LOCUS       DAZFRJ010000685          358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000685 DAZFRJ010000000
VERSION     DAZFRJ010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.691"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>358
                     /locus_tag="ACHKUN_005444"
     rRNA            <1..>358
                     /locus_tag="ACHKUN_005444"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          109 a           81 c          112 g           56 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag
      121 tatgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgt
//
LOCUS       DAZFRJ010000686          357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000686 DAZFRJ010000000
VERSION     DAZFRJ010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.692"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..357
                     /locus_tag="ACHKUN_005445"
                     /pseudo
     CDS             15..357
                     /locus_tag="ACHKUN_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          129 a           77 c           61 g           90 t
ORIGIN      
        1 aataaggatt tattatgaac attcaaccga ccatacaatc tggaatcacc tcacaaaaca
       61 atcaacatca tcaaacagaa caaataccct ctacacaaat accgcaatcc gaattacctc
      121 taggatgcca agctggattt gttgttaata ttccagatga tatacagcaa catgcaccgg
      181 aatgcggtga aacaacagct ctactgagct tgataaaaga taaaggtctg ctctcagggc
      241 tagacgaata tatagctcct caccttgaag aaggatccat aggaaaaaaa cattggatat
      301 gtttggttta ttcaatgtta cccaaatggc attagagata cctagttccg tttcagg
//
LOCUS       DAZFRJ010000687          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000687 DAZFRJ010000000
VERSION     DAZFRJ010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.693"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..311
                     /locus_tag="ACHKUN_005446"
                     /pseudo
     CDS             1..311
                     /locus_tag="ACHKUN_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          101 a           74 c           74 g          102 t
ORIGIN      
        1 gctgtaccgt tatttcatta ccatgctcga cgaacacagc aactactctc tggccgtact
       61 atcgcttaaa agtgatatca tcaggcattt cttctgctgt gtagcccagt tgttccttgt
      121 tggtatcaac cagatcatca ctgatgatgg gcaaagagtt ctcgggaaaa ttcgacaaaa
      181 cgctacagga agccaccgtc gggttcgtat ggacttatct ctatacgccg aaaataaatg
      241 ccataagtga gcattttaac cgaacactga gggaacaatt tattgagttt aatgaagaat
      301 tgctctttga atagctgaat atgttcaatc tgaaactggc tgagtatctg g
//
LOCUS       DAZFRJ010000688          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000688 DAZFRJ010000000
VERSION     DAZFRJ010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.694"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHKUN_005447"
     CDS             complement(<1..245)
                     /locus_tag="ACHKUN_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6663468.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..>349)
                     /locus_tag="ACHKUN_005448"
     CDS             complement(245..>349)
                     /locus_tag="ACHKUN_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6663469.1"
                     /translation="PYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT           76 a          122 c           71 g           80 t
ORIGIN      
        1 gacaccgtca gcgacggtct ggcactgctg cccttcccgt tcatctcaaa accgctgccg
       61 tcaatcgggt atacctgata ttgccgcccc tgccaggtga ccgcctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcaccg ccctgcaccg tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca gactttcttc gtgaatatcc
      241 tgcatcagtt caccacctgc ttaaactcgg cactgaattc aacccgcagc atgcgaaccc
      301 gtgatgacca ggcggcacag gtcaccttta tctgccgcca ggcataagg
//
LOCUS       DAZFRJ010000689          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000689 DAZFRJ010000000
VERSION     DAZFRJ010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.695"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(4..294)
                     /locus_tag="ACHKUN_005449"
     CDS             complement(4..294)
                     /locus_tag="ACHKUN_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO6663470.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
BASE COUNT           69 a          106 c           68 g          103 t
ORIGIN      
        1 tggtcatctt ttaactccat ataccgccaa tacccgtttc atcgcggcac tctggcgaca
       61 ctccttaaaa attaggttcg tgctcatctt tccttcccgt tcttccttgg tagcaaaccg
      121 gtaatacacc gttcgccaga ccttaccttc gataaccaga agacctgccc gtgccatttt
      181 agccgcggcc tgatttatgc tggttactgt tgcgcctgtt agcgcggcaa cgtccggcgc
      241 acagaagcta ttatgcgtcc ccaggtaatg aataattgcc tctttgcccg tcatacactt
      301 gctcctttca gtccgaactt agctttgagt tctgcgatct tcgcca
//
LOCUS       DAZFRJ010000690          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000690 DAZFRJ010000000
VERSION     DAZFRJ010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.696"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          119 a           69 c           60 g           92 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga
//
LOCUS       DAZFRJ010000691          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000691 DAZFRJ010000000
VERSION     DAZFRJ010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.697"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>337
                     /locus_tag="ACHKUN_005450"
     CDS             <1..>337
                     /locus_tag="ACHKUN_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO6663471.1"
                     /translation="LWVADFTYVSTWQGFVCVAFIIDVFAGCIVGWRVSSSMETTFVL
                     DALEQALWARRPSGTVHHSDKGSQYVSLAYTERLKEAKLLASTGSTGDSYDNAMAESI
                     NGLYKAEVI"
BASE COUNT           79 a           72 c          104 g           82 t
ORIGIN      
        1 agctgtgggt ggctgatttt acttacgtca gcacatggca gggcttcgtc tgtgtggcgt
       61 tcatcattga tgtgtttgcc ggatgtatcg tggggtggcg agtctcatcg tctatggaaa
      121 cgacattcgt gctggatgca ctggagcagg cgttgtgggc ccgtcggccg tccggcacag
      181 tccatcacag tgataaaggt tctcagtatg tatcactggc ctatacggag cgactaaaag
      241 aagcaaaact gctggcatca acagggagta caggtgactc gtatgacaac gcgatggcgg
      301 agagcatcaa tggtctttac aaagcggagg taataca
//
LOCUS       DAZFRJ010000692          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000692 DAZFRJ010000000
VERSION     DAZFRJ010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.698"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(7..>336)
                     /locus_tag="ACHKUN_005451"
     CDS             complement(7..>336)
                     /locus_tag="ACHKUN_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6663472.1"
                     /translation="SFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPK
                     ARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGG
                     QVVVIGR"
BASE COUNT           77 a          109 c           84 g           66 t
ORIGIN      
        1 tttgcatcag cgaccaatca ccacaacctg accaccgtcc ccttcgtctg ccgtgctgat
       61 ctcctgagaa accacgcgag accccacgcg catttccccg tacagaacag gcagaacatt
      121 gccctgggca accatgttat ccagtgagga gaaataggtg ttctgcttac cgttatccgt
      181 tgtctgtgta cggggagttc tggctttcgg tgccagcatc tgcgccacac caccgagcac
      241 catactggca ccgagagaaa acaggatgcc ggtcatacca ccggccccaa tggctgcccc
      301 ccatgctgca agggtggctc cggcggtaaa gaatga
//
LOCUS       DAZFRJ010000693          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000693 DAZFRJ010000000
VERSION     DAZFRJ010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.699"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>329
                     /locus_tag="ACHKUN_005452"
     CDS             <1..>329
                     /locus_tag="ACHKUN_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6663473.1"
                     /translation="LSALQRIEHLALLKRRAEQAEASGNLQVSVEDLVRTGAFLVAMS
                     LWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAEDVVLRLSGMSGAVHVDTDIT
                     EVAKNNAL"
BASE COUNT           75 a           83 c          106 g           65 t
ORIGIN      
        1 ctgtctgcgc tgcagcgtat cgagcatctt gccctgctga aacgacgggc agaacaggct
       61 gaagccagcg gtaacctgca ggtgagcgtg gaagaccttg tcagaaccgg cgcgtttctg
      121 gtggcgatgt ccctgtggca taaccatcca cagaaaacgg agtcaccatc aatgaatgag
      181 gctgtgatgc agatcgaaca ggaggtgctc accacctggc ctgctgatgc cattgcccgg
      241 gcggaagacg tggtgttgcg tctgtccggg atgagcgggg ctgttcatgt ggatacggat
      301 atcaccgaag tggcgaaaaa taacgcgct
//
LOCUS       DAZFRJ010000694          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000694 DAZFRJ010000000
VERSION     DAZFRJ010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.700"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          109 a           72 c           48 g           99 t
ORIGIN      
        1 agaaactaaa aaaacgtcag ggcgttgata ataatgaata ccaactttct gcccatttat
       61 agtaatatct ttcgttatca ggttttgtct ggcagcttca cgtaatattt caatactctg
      121 caaagcaaca tttgatgaaa tgctggatgt tatcaatggc atatgtcatt taccccaaca
      181 agactaaatt ttagaaaaaa caactgccgg gacaactttc gccccctgta accccaaata
      241 agatgtcaga tttatcggca ttaatttatc tccctgttga attagcacct ctatcatcta
      301 cttaacaaat atcccacgga cattgcaa
//
LOCUS       DAZFRJ010000695          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000695 DAZFRJ010000000
VERSION     DAZFRJ010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.701"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>327
                     /locus_tag="ACHKUN_005453"
     CDS             <1..>327
                     /locus_tag="ACHKUN_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO6663474.1"
                     /translation="FPEDTYDSRARARSLEYIEQNYNEKEICPYAFMVYGVGDGGAGP
                     GEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIISGELYFEAHQGCFTSE
                     SATKAH"
BASE COUNT           86 a           76 c           73 g           92 t
ORIGIN      
        1 actttcctga agatacgtat gatagccgag cccgcgctcg ctctttagaa tatattgaac
       61 aaaactataa tgaaaaagaa atctgtccct atgcgtttat ggtgtatggt gtcggtgatg
      121 gcggcgctgg ccccggtgag gaacatatag aacgattaac acgcatacgc aatattgatg
      181 gcttacctca tgttgatttc agccgtgttg ataagttctt cactcacgcc gacgcctttc
      241 gcgaatcctt gcccattatt tcgggtgaac tttacttcga ggctcatcaa gggtgcttta
      301 catcagagtc agcaactaaa gcccata
//
LOCUS       DAZFRJ010000696          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000696 DAZFRJ010000000
VERSION     DAZFRJ010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.702"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..167)
                     /gene="tnpB"
                     /locus_tag="ACHKUN_005454"
     CDS             complement(<1..167)
                     /gene="tnpB"
                     /locus_tag="ACHKUN_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO6663475.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKL"
BASE COUNT           72 a           83 c           90 g           81 t
ORIGIN      
        1 agctttacct gactgccatt acgcccacgg aagataaaaa cgtgacctga catcggatcg
       61 tctttcagcg tcgtctgcac ctttgccgcc aggccgttga agccgtttct catatcggtg
      121 atgccagcga ccagccagat ctttgtccct gatggtaatg ggatcaacgt ttgagctccc
      181 gtatcagcag gttcaggata ttttcgctga tggcaccatt cagacggagt gatccgtgcc
      241 ggaacgtcac ttcacagctg atattgagtg attcaggtgt cgcagcagct acaggttgtg
      301 accgggggga ggttgtaggg acaaca
//
LOCUS       DAZFRJ010000697          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000697 DAZFRJ010000000
VERSION     DAZFRJ010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.703"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>322)
                     /locus_tag="ACHKUN_005455"
     CDS             complement(<1..>322)
                     /locus_tag="ACHKUN_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6663476.1"
                     /translation="TEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPATVGSWLS
                     RWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRGAPVQVRA
                     LEQWM"
BASE COUNT           78 a          106 c           73 g           65 t
ORIGIN      
        1 tcatccactg ctcaagtgca cgtacctgta ccggtgcacc acgaccggcc tcaccggctt
       61 caaaaatcag ccgccagagt ggttcctcct gccagaaaaa ccggtcaaat gatgacagtg
      121 atggaaaacg cccgcaccac ccccagaaaa tcgtttccag atcatgctcc tcaacaacac
      181 cagaccagcg ggacaaccag cttccgaccg tggcgggagt atgaaacgca aggtcaatct
      241 gcgcctgagc aaaacatgtc tggtattcca gttcccgtcg ttctgcctca cgttcccgct
      301 ggcggctgta tttctgctct gt
//
LOCUS       DAZFRJ010000698          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000698 DAZFRJ010000000
VERSION     DAZFRJ010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.705"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>314
                     /locus_tag="ACHKUN_005456"
     CDS             <1..>314
                     /locus_tag="ACHKUN_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO6663477.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZFRJ010000699          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000699 DAZFRJ010000000
VERSION     DAZFRJ010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.706"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>314
                     /locus_tag="ACHKUN_005457"
     CDS             <1..>314
                     /locus_tag="ACHKUN_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6663478.1"
                     /translation="RLSGRETFRQLADKTGVSKTAIRRLSGALAASGDVWLSGCGVFP
                     SEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQNIICRECRQSEVMQRVLAFYRG
                     NF"
BASE COUNT           73 a           71 c           96 g           74 t
ORIGIN      
        1 gcgcctgagc ggacgagaaa cttttcgaca actggcagat aaaacgggtg tcagtaagac
       61 cgctattcgt cgtttatctg gtgcgcttgc ggccagtggt gatgtctggc tctctggttg
      121 cggggtattt ccatcagagc aggcgtatcg cgtatggcgt aagacaccgg agaaggctgc
      181 tgacccgaca ctgattcgaa agttacctga cggagaaata cgtcgttaca acagacggca
      241 gaacataatt tgtcgtgagt gccgccagag cgaagttatg cagcgtgtgc tggcgttcta
      301 tcggggaaac tttc
//
LOCUS       DAZFRJ010000700          313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000700 DAZFRJ010000000
VERSION     DAZFRJ010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.707"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..229
                     /gene="fumD"
                     /locus_tag="ACHKUN_005458"
     CDS             20..229
                     /gene="fumD"
                     /locus_tag="ACHKUN_005458"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO6663479.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          101 a           56 c           82 g           74 t
ORIGIN      
        1 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
       61 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
      121 tattgttatc gcaggcgtat tacgtacagc attagccaat aaacgcattc agcgcagtga
      181 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
      241 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
      301 ataacaactg ctg
//
LOCUS       DAZFRJ010000701          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000701 DAZFRJ010000000
VERSION     DAZFRJ010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.708"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           96 a           74 c           57 g           85 t
ORIGIN      
        1 agcatttgta gcttttaaca tctcaatgta agagatggca agattatggg ggcgcggtat
       61 tatcaacctc cccccctaaa aacctctttc cgatgccact ctacaacccc ctaaaaaacc
      121 ggtggcgatt cacattccac catgcattcc gattaacgcc gcatagccag ttgaactttg
      181 ctactctgtg agaggtagta ccttctatcc agtgcgaatt taattaatgg aataaatgat
      241 tatgagtgaa agtgatacaa tcccaaagaa gtctacaagt cagattaaca aagcggtatt
      301 ctttacatct gc
//
LOCUS       DAZFRJ010000702          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000702 DAZFRJ010000000
VERSION     DAZFRJ010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.709"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>312)
                     /locus_tag="ACHKUN_005459"
     CDS             complement(<1..>312)
                     /locus_tag="ACHKUN_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6663480.1"
                     /translation="PQVLELAIGWRGALSESIKGVKFCIWFSVAWRTIEFIISSERDL
                     VNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLN
                     EI"
BASE COUNT          107 a           79 c           51 g           75 t
ORIGIN      
        1 gatttcattt aaaacaactg tacacactat ccctgtcagt aaaattgctc ctccgacaag
       61 aattactgga aagccaaagc taacacaggc aaaagaagcc aggcttccaa tagcggttgc
      121 aacaccaccg gctataactg ctttggccac atccattgaa aaatcgccaa gaaaattaac
      181 cagatctcgc tctgaactta taataaattc tatggttctc caggctactg aaaaccagat
      241 acagaactta actcctttta ttgattcaga taaagctcct cgccagccaa tagcgagttc
      301 cagaacttga gg
//
LOCUS       DAZFRJ010000703          300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000703 DAZFRJ010000000
VERSION     DAZFRJ010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.710"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..>300
                     /locus_tag="ACHKUN_005460"
     CDS             2..>300
                     /locus_tag="ACHKUN_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFO6663481.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHD"
BASE COUNT           98 a           62 c           44 g           96 t
ORIGIN      
        1 tatgcaagtc ttttccagtg atgtctactt cactgtaggc actaatgcat tattagcatc
       61 ccaaaaagaa tattacagcg atttagttgc gttggttgat cttggacatt cgttcgttgt
      121 tattgatgag catcaacatc gaaatctcaa accaaataca gaacctgtaa acatcctttt
      181 gagcaacaat ttcatcagga tcaataagaa tattacatta tctgatttaa cgcatttcct
      241 ggtttcaaat cttcatacac aaaatgtgta ttccacacaa gaaccactta cccatgatga
//
LOCUS       DAZFRJ010000704          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000704 DAZFRJ010000000
VERSION     DAZFRJ010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.711"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           89 a           49 c           63 g           92 t
ORIGIN      
        1 cgcaagtgtt cgtttggctt cacatggcat cttcttctta gaaaaatatc gacatatttt
       61 gtgacacgaa ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata
      121 acaggtataa aggtgcacca agatagtcaa tgagacaggg catctcgcaa tctatggcaa
      181 acatcacttc agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat
      241 gaaaatgaga tcaacagaca ctctatttta tgactctggg taaaatggat tga
//
LOCUS       DAZFRJ010000705          291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000705 DAZFRJ010000000
VERSION     DAZFRJ010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.713"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>291)
                     /locus_tag="ACHKUN_005461"
     CDS             complement(<1..>291)
                     /locus_tag="ACHKUN_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6663482.1"
                     /translation="QVYPYVVPLLDGRPSVALPWVVFSLISSVSADVMGGQAESSVSV
                     QIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENRCYRATLEFQV"
BASE COUNT           65 a          101 c           75 g           50 t
ORIGIN      
        1 tgacctgaaa ctccagggtt gcacggtaac agcggttttc cggaatatag tcctgcattt
       61 cactgacgga tcccggggcc agcagcatta tggcttcacg ggcgtcctga cgtatctgac
      121 gcgcctgcgt cacagtcccg gcataaacgt ctatctgcac cgacactgag gactccgcct
      181 gcccgcccat cacgtccgcc gacaccgatg aaatcaggct gaaaaccacc cacggaagcg
      241 ccaccgacgg cctgccatcc agcaggggga ccacatacgg gtacacctgc c
//
LOCUS       DAZFRJ010000706          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000706 DAZFRJ010000000
VERSION     DAZFRJ010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.714"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..>288
                     /locus_tag="ACHKUN_005462"
     CDS             2..>288
                     /locus_tag="ACHKUN_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO6663483.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEE"
BASE COUNT          115 a           56 c           69 g           48 t
ORIGIN      
        1 tatgtctgtc acaattcagg gaaatacctc aaccgttatt tcaaacaact ccgccccgga
       61 aggaacatca gaaatagcca aaatcacaag acaaattcag gtgctgactg aaaagcttgg
      121 gaaaatctca tcggaagagg ggatgacgac acagcagaaa aaagaaatgg ctgcattggt
      181 acagaagcaa attgaaagcc tcagggctca actggagcag ttgttaaggc agcaggcaga
      241 gaaaaagaat aaagacgcga cagttcagcc tgataaaaaa gaagagaa
//
LOCUS       DAZFRJ010000707          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000707 DAZFRJ010000000
VERSION     DAZFRJ010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.715"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>287
                     /locus_tag="ACHKUN_005463"
     rRNA            <1..>287
                     /locus_tag="ACHKUN_005463"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           67 c           80 g           53 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgc
//
LOCUS       DAZFRJ010000708          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000708 DAZFRJ010000000
VERSION     DAZFRJ010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.716"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>287)
                     /locus_tag="ACHKUN_005464"
     CDS             complement(<1..>287)
                     /locus_tag="ACHKUN_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6663484.1"
                     /translation="FVLLTGIAELIALLCGHSLSSAMDTPDLPEGLTGEALDWLNIGR
                     IPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAPLCLLLTCPLVH"
BASE COUNT           88 a           67 c           87 g           45 t
ORIGIN      
        1 ggtgtacgag aggacatgtc agcagcaggc aaagtggtgc aaggagaatg ttcggggcag
       61 gagcctgcag cagatgaata ataagaccct gcaggagaat gccggatata ccaaaaaaac
      121 ctgccagcat acagaggacg atcagcaggg ggatccgtcc gatgttcagc cagtccagcg
      181 cctctcctgt cagcccctcc ggtaaatcag gtgtatccat agctgaagag agggagtgtc
      241 cgcagagcag ggcgataagt tcagcgatac cagtaagcag aacaaaa
//
LOCUS       DAZFRJ010000709          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000709 DAZFRJ010000000
VERSION     DAZFRJ010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.717"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>284
                     /locus_tag="ACHKUN_005465"
     CDS             <1..>284
                     /locus_tag="ACHKUN_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFO6663485.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAA"
BASE COUNT           81 a           67 c          101 g           35 t
ORIGIN      
        1 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
       61 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
      121 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
      181 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
      241 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agcc
//
LOCUS       DAZFRJ010000710          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000710 DAZFRJ010000000
VERSION     DAZFRJ010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.718"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>281)
                     /locus_tag="ACHKUN_005466"
     CDS             complement(<1..>281)
                     /locus_tag="ACHKUN_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HFO6663486.1"
                     /translation="YLDSLDERGLILSLAAFSEDALGKMLLTFMLDNKASKELIEGFN
                     APLGTFSSRIKACFSLGLITEGQYKDLELLRKIRNKFSHSWENISIEDQ"
BASE COUNT           81 a           55 c           52 g           93 t
ORIGIN      
        1 tcctgatcct caattgaaat gttttcccag ctgtgagaga atttatttct tatttttcta
       61 agaagttcaa gatctttgta ttgaccctct gttattagtc ccaaggaaaa acaggctttt
      121 attcgtgaag aaaatgttcc aaggggggca ttaaaccctt cgattaattc tttagacgct
      181 ttgttatcga gcatgaacgt caaaagcatt ttacctaagg cgtcttcaga gaaagccgct
      241 agagacaata ttaggcctct ttcatctagg ctgtccaaat a
//
LOCUS       DAZFRJ010000711          274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000711 DAZFRJ010000000
VERSION     DAZFRJ010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.719"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            65..>274
                     /locus_tag="ACHKUN_005467"
     CDS             65..>274
                     /locus_tag="ACHKUN_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6663487.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYAL"
BASE COUNT           95 a           66 c           56 g           57 t
ORIGIN      
        1 ggtaacgtac gaagcacagc catgtaaaat cctcgccata tagcttgtca ccagaggaaa
       61 gaaaatgtca tcgaaaaacc ggccccgcag aacaacaacc cgcaacatcc gatttccaaa
      121 ccagatgatt gaacaaatta acatcgctct tgaccagaaa ggttcagaaa atttttctgc
      181 gtgggtcatt gaatcttgcc gccgggagct ggcagcagac ataaaatatg cccgtcagtt
      241 gactataaaa aagaatgata cacagtatgc tctg
//
LOCUS       DAZFRJ010000712          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000712 DAZFRJ010000000
VERSION     DAZFRJ010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.720"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..95
                     /locus_tag="ACHKUN_005468"
     tRNA            19..95
                     /locus_tag="ACHKUN_005468"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHKUN_005469"
     tRNA            138..213
                     /locus_tag="ACHKUN_005469"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           71 a           62 c           73 g           67 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aag
//
LOCUS       DAZFRJ010000713          272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000713 DAZFRJ010000000
VERSION     DAZFRJ010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.721"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>272
                     /gene="gpFI"
                     /locus_tag="ACHKUN_005470"
     CDS             <1..>272
                     /gene="gpFI"
                     /locus_tag="ACHKUN_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6663488.1"
                     /translation="ELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDTDDAA
                     GQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDAL"
BASE COUNT           65 a           68 c           89 g           50 t
ORIGIN      
        1 gaactgattg cccgtctccg ctcgctgggt gaacaactga accgtgatgt cagcctgacg
       61 gggacgaaag aagaactggc gctccgtgtg gcagagctgg aagaggagct tgatgacacg
      121 gatgacgcag ccggtcagga cacgtctgtc agcccggaaa atgcgctgac cggacatgaa
      181 aatgaggtgg tatcagcaca gacggatacc gtgactgata cggctgctct ggtcacggtt
      241 gtggcactgg tgacgctgca taccgatgca ct
//
LOCUS       DAZFRJ010000714          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000714 DAZFRJ010000000
VERSION     DAZFRJ010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.723"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          100 a           65 c           25 g           80 t
ORIGIN      
        1 caacctaaaa aacaacccca tacatataaa ttattttata ggtaaaatag attatatatt
       61 ctcatagcta ccataaacta tactagcctg aactatattt attctgctgc aatcaatgca
      121 tacataacac aaatatcact caggtacact actcaaacca cgctgggatt tttcctcaag
      181 ttataatcat caccccgaaa atcattcggc atttactcat taaatagtca ccccataggc
      241 ctgtacatgt ttactcagaa atatacatcc
//
LOCUS       DAZFRJ010000715          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000715 DAZFRJ010000000
VERSION     DAZFRJ010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.denovo.724"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>267
                     /gene="tccP2"
                     /locus_tag="ACHKUN_005471"
     CDS             <1..>267
                     /gene="tccP2"
                     /locus_tag="ACHKUN_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096071309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO6663489.1"
                     /translation="NSFFSSIPHSISSVTRNSSFTASQHKSTPNTVKTSSPLSPSNSP
                     ASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
BASE COUNT           84 a           75 c           37 g           71 t
ORIGIN      
        1 taattctttt ttttccagca ttcctcacag tatatcaagt gttacgcgca acagtagctt
       61 cactgcctca cagcacaaaa gcacacctaa cacggtaaaa accagctcac ctctttctcc
      121 cagcaacagt cctgccagtg caacaactat ttttaaggta aaaaattctt atacggaatc
      181 aggacttcaa cgttccacat cttatactca gtcaagtata gaaaaaaacg ctttacatcg
      241 gcctctacct gatgtggcca aacgcct
//
LOCUS       DAZFRJ010000716        15312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.guided.106, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000716 DAZFRJ010000000
VERSION     DAZFRJ010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.guided.106"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            381..1295
                     /gene="lafA"
                     /locus_tag="ACHKUN_005472"
     CDS             381..1295
                     /gene="lafA"
                     /locus_tag="ACHKUN_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO6663490.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            1502..2818
                     /gene="fliD"
                     /locus_tag="ACHKUN_005473"
     CDS             1502..2818
                     /gene="fliD"
                     /locus_tag="ACHKUN_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6663491.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            2841..3233
                     /gene="fliS"
                     /locus_tag="ACHKUN_005474"
     CDS             2841..3233
                     /gene="fliS"
                     /locus_tag="ACHKUN_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6663492.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            3238..3549
                     /locus_tag="ACHKUN_005475"
     CDS             3238..3549
                     /locus_tag="ACHKUN_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO6663493.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            3546..4607
                     /locus_tag="ACHKUN_005476"
     CDS             3546..4607
                     /locus_tag="ACHKUN_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6663494.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            4615..5082
                     /locus_tag="ACHKUN_005477"
     CDS             4615..5082
                     /locus_tag="ACHKUN_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO6663495.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            5102..5818
                     /locus_tag="ACHKUN_005478"
     CDS             5102..5818
                     /locus_tag="ACHKUN_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO6663496.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            5831..6694
                     /gene="motA"
                     /locus_tag="ACHKUN_005479"
     CDS             5831..6694
                     /gene="motA"
                     /locus_tag="ACHKUN_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6663497.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            6697..7620
                     /gene="lafU"
                     /locus_tag="ACHKUN_005480"
     CDS             6697..7620
                     /gene="lafU"
                     /locus_tag="ACHKUN_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6663498.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            7691..8746
                     /gene="dinB"
                     /locus_tag="ACHKUN_005481"
     CDS             7691..8746
                     /gene="dinB"
                     /locus_tag="ACHKUN_005481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6663499.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            8743..9195
                     /locus_tag="ACHKUN_005482"
     CDS             8743..9195
                     /locus_tag="ACHKUN_005482"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6663500.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            9441..10580
                     /locus_tag="ACHKUN_005483"
     CDS             9441..10580
                     /locus_tag="ACHKUN_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6663501.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            10577..11191
                     /gene="prfH"
                     /locus_tag="ACHKUN_005484"
     CDS             10577..11191
                     /gene="prfH"
                     /locus_tag="ACHKUN_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6663502.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(11248..12705)
                     /gene="pepD"
                     /locus_tag="ACHKUN_005485"
     CDS             complement(11248..12705)
                     /gene="pepD"
                     /locus_tag="ACHKUN_005485"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6663503.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            12966..13424
                     /gene="gpt"
                     /locus_tag="ACHKUN_005486"
     CDS             12966..13424
                     /gene="gpt"
                     /locus_tag="ACHKUN_005486"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6663504.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            13516..14760
                     /gene="frsA"
                     /locus_tag="ACHKUN_005487"
     CDS             13516..14760
                     /gene="frsA"
                     /locus_tag="ACHKUN_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6663505.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            14818..15219
                     /gene="crl"
                     /locus_tag="ACHKUN_005488"
     CDS             14818..15219
                     /gene="crl"
                     /locus_tag="ACHKUN_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6663506.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         3856 a         3801 c         4136 g         3519 t
ORIGIN      
        1 attttatggt aataggatta ttcttaatca ctatatggca tggaaatgta acgaaatgac
       61 aggactggta caatagccaa atgtgtacta tttgcaacgc aatgtaaatt ttggcatcag
      121 tacattgaga agaaaacgcg ttaattgatg gtaatcttaa ctatatcaag cgtttgataa
      181 agacatattt tgatacctgg ccatgtggca gatattgcta ataatctttt tatctgaatt
      241 taaactctgc tttttttagc taaaagtggc atcagataaa tcgcagtgaa atttttttaa
      301 cggcggtttt aagtttgctc cttaggtgtc gtttttacag ggtatcgaaa cgaaaacgat
      361 taatcccaag gagcagtatc atgttatcta ttaacaccaa taacgcctca atggccgccg
      421 ttaatgcaat cagcaaaagc agctcttctt tgtctacatc tatggaacgc ctggcgacag
      481 gtaaccggat taactcttct gccgatgacg ccgcaggcaa acaaattgct aaccgtctga
      541 cagcacagtc cagcggtatg ggcgtggcgc tgagcaacat caatgacgca accgcgatgc
      601 tgcaaaccgc cgatagtatg ttcgatgaaa tgtctgatgt gctgggtcgt atgaaagacc
      661 tgtcaactca ggcagcgaac ggcacctaca gcgacgacga tctgcaggcg atgcaggacg
      721 aatacgacga actgggtcag cagatgtctg acatgctgca aaacaccact tacggcggca
      781 ccaacctgtt cggtgtttcc ggtaccagca acaccggtac tgacggtctg ttccagagcg
      841 cggtaacttt ccaggtgggc gcggaatctt ccgacaccat gaccgtgaac atctccagcc
      901 aactgaacac gctggtgacc gatctttctg ctatcagcaa ctcctttagc gcggatcagg
      961 cagacaccac gggtactgca ggtgtttctg gcggtactga gctgaccgcg tctggctcgg
     1021 ctaaccagat gatcaccagc atctctaccg cgatggatga cgtttcgcag atccagtcca
     1081 agctgggtgc gtctatcaac cgtctgaacg acaccgccaa caacctgacc agtatgcagg
     1141 acaacaccga agtcgctatc ggtaacatta tggataccga ctacgcgacg gaagcgtcca
     1201 atatgaccaa acagcaggtg ctgatgcaga ctggcatcac catgctgaaa cagtccaaca
     1261 gcatgtccag catggtttcc agcctgctgc agtaatggcg gacttgccgg atgcggcgta
     1321 aacgccttat tcggactacg aacaacaccg cctgcgggcg gtgttgtgct ttctgcggca
     1381 ggcttttttc cgccagaccg gaaacgccat ttccgcctgt tttttaatct attgattatt
     1441 cacataaaaa atattggcac gccttttgcc aataacctga catcgttatc cggagtcatc
     1501 aatgatcaac ccaagaacca ttgcgcaaga aatcgcgtat gcggatgtcg ccactcaggc
     1561 ggcaaacttg caggagaagc aaacagagct ggatgctgaa agcagcggcc tggattcgct
     1621 cagttcagcg ttgagcgatt ttcagagcgc cgttgacgcg ctgaacagcg ataccgacgg
     1681 cccggtgacg tttgccgcca ccagcaataa tgactcggcg agcgtttccg ccaattccca
     1741 ggcgcaggcg ggaagctact catttttcgt tgagcaactg gcacaggggc agcaaaccac
     1801 gttcagtatg ggcgacgacg ccttttccgc caccggcact tttgaactga cgatgggcga
     1861 cagcaccatg gatatcgatc tgtcggcggc agatcagaat ggtgacggcg acggttttat
     1921 cgacgccagt gaactggtga atgcgattaa cgactccgat gataatccgg gcgtgtcggc
     1981 ggcgctggta aaaaccgacg gcaccacaac gattatgctc acctcggata gcaccggggc
     2041 gcagagcgcg ttttcggtga gcgtaacggg gcatgatgcc agcaacgata gcaccagcgc
     2101 gcccgttgcc acggatgttt cctccgctca ggacgcgatt attcatcttg gcagcgccac
     2161 ggggcctgag atcaccaaca gcagcaatac gtttgatgat gtgattcccg gtgtcaccat
     2221 gactttcacc gaagtcagcg attctgacag cgatctcact acctttaaca tcagcgaaga
     2281 ttccagcgcc agccaggaga aagtacagac ctttgtcgat gcctataaca ccttgattga
     2341 tacggttgat tcgctaacca cccacggtga tgacagtacc agcgccgggg tatttgcagg
     2401 cgacgccgga ctcagttcac tggcgaacca gctcgatgac atcgcccatg ccagctacaa
     2461 cggcgtgtcg attgttgact atggcatcac cctcgattct cacggccact tacagattga
     2521 ctccgaccag ttcaacgacg aaatggcgaa aaatcctgac ggtctgacct ctattttcgt
     2581 tggcgacaac agcatggtgg cgcagatgga cgaccttatt aacacctaca cggactccag
     2641 caacggcatc atcaccctgc gccagcagaa cattgacgat cagatgagca aaattcagga
     2701 cgaaggcgat cagcttactg atacctataa cgccaactac gaccgttatc tcgaggagta
     2761 caccaacacg ctggtcgagg tgtacaccat gaaagccagc atggcggcat tcgcgtaact
     2821 cagaaggaat atccctaaag atgtaccaag tacaggacgg ctattcgcaa tataaagaga
     2881 tcgatctcgc ggcgcgtact gcggcggcat cgcctctgga actggtgctg gtgctgtttt
     2941 ctggcctgat ggacgaactg gaacgcgcca aaagccatat tgaagggcgg cgtttcgaga
     3001 agaaagccca gagcatcaat aaatgcatcg acattctcaa tgcgctcacc agttcgctgg
     3061 agtttgaaac tggcggcgag ctggtggtca acctttcgcg actctatgac cactgcgttt
     3121 accgattgta tgaggccagc ggcgaactgt cggcagagaa aatcgatgaa gtgatgctga
     3181 tcttaagcaa cctgcgcgaa ggctgggaag ggctttcggg caagctgggt taagacgatg
     3241 gaaaaacaac gcaggaaact tttcgcactg gtcgaggcga tgaatgaagc cctcgacaag
     3301 caacgctggc gacgccttcc ggcactgcat cagcagttga tacgccagtt tcacgattac
     3361 gcggcggcgg aaaccgatgc ggcgcagttg gatgcagtga aagcgcgact gtatgacgcg
     3421 tttaacgccc tgattaaacg gcgcgagcag cgcgcagaag tgttgaaagc gcgtatggaa
     3481 cagcatcagc ggcatcagga aggcgtactg gcctactcaa ttgttaattt actttcggag
     3541 aagtcatgaa tccgacattg cttgccacgc tcggaacgct tgcggaaacc gcttcgttga
     3601 aggcggatat tttaccgccg gtgagcggtg aaaacgcgcc cgcgtttacc ctgccgaaaa
     3661 tggcggtcgc ggcggtggcg gagcgcgttc atagcgcaaa aaccagtcag caacaggcga
     3721 cgcgcccgca ggagaacgat ccggtggcga tgcaggcgct aatggcgctg ttacttccgc
     3781 aacctgccgc gccgcatcag gacacgccgc agccgcgaaa cgtcgcaaca tcgccggtta
     3841 tccagcaatt gacgaaagcg gtggtgcaaa acgcgccgca acgccagacg caacagcagg
     3901 aactcacgcc gttgccgccg cagttgcagg aactgatcag tcagttgccg caggcgaagc
     3961 ccgaacagca ggccagactg gcgacttacg ctagtgaaga tttacatgcc attacgccga
     4021 cgcagccgcg cgtctcaaca cagccagctc gcccgaaatc tgaactaacc cgtgtgaccg
     4081 cgcgcccgca ggtcgagcgt aaaacggaaa aagtgccgga cagtgaaccg gttattgcgc
     4141 gtgcggtgtt gcaggttaag acgccggagc tgctcagcga tcatcaggag attgtcgcca
     4201 aaccagccac gctttcgatg gacgaactgg gcgaaaaact gacgacgctt ttgaaagatc
     4261 agatccactt tcagctcaac aaacaacagc agatctccac catccgtctc gatccaccgt
     4321 cgcttggcaa gctcgagatc gccgtacaac tcgacaacgg caaactgatg gtgcacatcg
     4381 gcgcgaacca aagtgaagtt tgccgcgcgt tacagcagtt tagcgacgat ctccgccagc
     4441 atctgacggc gcaaaatttt atggaggtga acgtacaggt ttcctccgaa gggcagtcgc
     4501 agcaacaaca acagtcgggc catcagcagg aagaggtgag tgccgcctta cagcttgatg
     4561 atgcgcctca atttcaacag aacgaatccg ttttgatcaa agtgtaaagg caatatgaag
     4621 aaaatcgtgg tggcgggcgt ggtcagcgcc gttctggcgt tggtggtggg ggccggagct
     4681 ggatggggtg tctggcatca ttatgccgga aaaggaaaac cgactgccgc cgcgcagacg
     4741 gaaacggtgg aaaccctgga tgaaagcaaa agcgtgtttg tgacgttgcc ggaaaccatt
     4801 gtcacgctgc atgacaacaa cggcgcggac cattatttgt cggcggaatt agtgatggtg
     4861 gtcgccagtg acaaagaagc ggaaaaaata aaacatcagg agccgctgta tcaaagtatt
     4921 gcggttgaat gcctgacgga aatgaaattt gaagatttgc gcggcatgaa aatatctgcc
     4981 atccgcaaac tcatttctga cgcgttaaag aaagatcttc agcgtcgcaa aatgagcgca
     5041 ccgtataagg atttgctggt gaaaaaagtg gtcttccagt aatcaccagg cggatataaa
     5101 catgctacag gatgtattcg agtcagaaga atttgctgcc gcgccggtat taaccccgca
     5161 gcaggagagc cactacttgc aggcctattt accgttggtg cgtaaagtgg tgcggcaact
     5221 tgccccgcag tgtaattgca ttattgatcg tcaggatatg gaacaaacgg cgttaatggg
     5281 gctgcttaat gccatccgcc gctacgggct gccggacgaa ggttttgcgg gctatgccgt
     5341 gcaccgtatt cgcggcgcga tcctggatga gctgcgcgct ctcgactggc gaccgcgcca
     5401 gctgcgacag aaatatcatc agatgaaaga tctgattcgc gaaacgcgca aaaaactggg
     5461 gcacgaaccg gagtggtcag aactggccgt ggaagggatc tcacatgaag attatctcga
     5521 atatcagcaa ctggaagggg cggaaacgct cgccagcctg gacgaactgc tgaacggcga
     5581 agggccaggc attccgcttg ttggccggga gctggaagat cagtttgtca ctcagcaaat
     5641 gctgcaacag gcgctttcac aattaagcga aaaagagcaa ctgatcctct ccatgtatta
     5701 ccagcatgag atgaatctca aagagattgc gctggtatta gggctaaccg aggcccgaat
     5761 ttgtcaactg aataaacaga tcgcgcagaa aattcgtgat tttgtctatc cgaattaaag
     5821 tgaagaaatc atgcaaaaaa tattaggttt actggtggtg ctcggctgcg tatttggcgg
     5881 ttattttgaa gcgggcgggc aattagtggc tatctggcaa ccggcggaga tgatcattat
     5941 tttgggtgcc ggtttcgggg cgatgattat tggtaacccg aaacatgtgc tgaaagagat
     6001 tgcccatcaa attaaaggtg tgatcagtaa aaaacagctc gggccggagt ttcagcgtca
     6061 gctattaatg tgcctgtatg agttgctcga aatggtgcaa aacggtggtc tgcgcatgct
     6121 ggatcaacat attgagcaac cggaagagag caccattttc cagaaatacc cgctggtgtt
     6181 aacccagaag cggctggtga cctttattgc cgataacttt cgcctgatgg cgatgggtaa
     6241 aatcgacgcc catgaactgg aaggcattct cgatcaggaa ctggacactg cggaagagtc
     6301 gctgctcacg ccgtcgcgct cgctgcaacg taccgccgaa gcgatgccgg gatttggtat
     6361 ttgtgcagca gtgctgggga tcatcatcac catgcagtcg attgacggct ctattgccct
     6421 gatcggcctg aaagtggcgg cagcgctggt ggggacgttt ttaggcgtct ttatctgcta
     6481 ctgcctgatg gacccgctcg ccaacgccat ggaacaacag gcgcgggcag aacattcttt
     6541 gctggaatgc gtgcgcactg tgctggtggc gcaggcaggc ggcaaaccga cgctgctggc
     6601 ggtggatgcc gggcgtaagc tgctgcatct ggcgtccaaa ccgaccttcg ccaacctcga
     6661 cgcgtgggtg aatgcgatgc tggagcaaga ataagcatga gaaagacggg caaccgccgc
     6721 gagcgtgggg caaaaaccac catcgtccgg cggcagatca aaaagaatca tgccgggcat
     6781 catggcgggg cgtggaaggt ggcgttcgcc gactttaccc tggcgatgat ggcgctgttt
     6841 atgacgctgt ggattgtcaa cagcgtcagt aaatccgagc gtgaaagcat tatcgccgcg
     6901 ctgcacgggc agtcgatttt taacggtggc gggctgtcgc cgttaaataa aatcagcccg
     6961 tctcatccgc caaaacctgc caccgtggcg gtgccggaag agacggagaa aaaagcgcgt
     7021 gatgtgaatg agaaaacggc gctgctgaag aagaaatccg ccaccgagct tggcgagctg
     7081 gcaaccagta tcaacaccat tgcccgtgat gcgcatatgg aagcgaatct ggagatggag
     7141 attgttcccc agggattacg cgtactgatt aaagacgacc agaatcgcaa tatgttcgaa
     7201 cgcggcagcg cgcagattat gccgttcttt aaaacgctgc tggtggagct cgcgccagtt
     7261 ttcgactcgc tcgataacaa aattattatt accgggcata ccgatgcgat ggcttacaaa
     7321 aacaatatct acaacaactg gaacctttcg ggtgaccgcg cgctttcggc tcgtcgggtg
     7381 ctggaagagg ccggaatgcc ggaagataaa gtgatgcagg taagcgcaat ggcggaccag
     7441 atgctgctgg attccaaaaa tccgcaaagc gcgggcaacc ggcgcattga gattatggtg
     7501 ctgaccaaaa gtgcgtccga tacgctgtat caatactttg gtcagcatgg ggataaagtg
     7561 gtgcagccgc tggtgcaaaa gctggataag cagcaggtgc tttcgcagcg aacgcgttaa
     7621 atgctgaatc tttacgcatt tctcaaaccc tgaaatcact gtatacttta ccagtgttga
     7681 gaggtgagta atgcgtaaaa tcattcatgt ggatatggac tgctttttcg cagcggtgga
     7741 gatgcgcgac aatcccgccc tgcgcgatat ccctattgct attggcggca gccgcgaacg
     7801 tcggggggtg atcagcaccg ccaattatcc cgcgcgtaaa tttggcgtac gtagcgctat
     7861 gccgacaggg atggcgctca aattatgccc gcatctcacc ttgcttccgg ggcgctttga
     7921 cgcctacaaa gaagcctcaa atcatatccg tgaaatcttc tcgcgctaca cctcgcgtat
     7981 tgaaccgttg tcactggatg aggcttatct cgacgtcacc gatagcgtcc attgccacgg
     8041 ttctgcgacc ctcatcgccc aggaaatccg ccagacgatt ttcagcgagc tgcaactgac
     8101 agcgtctgcg ggcgtggcac cagtaaagtt tctcgccaaa atcgcctccg acatgaataa
     8161 acccaacggt cagtttgtga ttacgccggc agaagttccg gcatttttac aaaccttacc
     8221 gctggcaaaa atccccggcg tcggcaaagt ctcggcggca aaactggaag cgatggggct
     8281 gcggacctgc ggtgatgtac aaaagtgtga tctggtgatg ctgcttaaac gctttggcaa
     8341 atttggccgc attttgtggg agcgtagtca ggggattgac gagcgcgacg ttaacagcga
     8401 acggttacga aaatccgtcg gcgtggaacg cacgatggcg gaagatattc atcactggtc
     8461 tgaatgtgaa gcgattatcg agcggctgta tccggaactt gaacgccgtc tggcaaaggt
     8521 aaaacctgat ttactgatcg cccgtcaggg agtgaaatta aagtttgatg attttcagca
     8581 aaccactcag gagcacgtct ggccgcggct gaataaagct gacttaatcg ccaccgcgcg
     8641 taaaacctgg gatgaacgcc gcggcgggcg cggtgtgcga ctggtggggc tgcatgtgac
     8701 gttgcttgac ccgcaaatgg aaagacaact ggtgctggga ttatgaataa catacaaata
     8761 agagactatc agcctggcga ttttcagcaa ctatgcgcta ttttcattag agcggttacg
     8821 atgactgcca gtcagcatta ttcaccacaa caaattgccg cctgggcgca gattgacgaa
     8881 tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagtcat taatgcaaaa
     8941 ctggtcggct ttattacctg cgttgaacac tatatcgata tgttatttgt tgaccctgaa
     9001 tacacccgcc gtggggttgc cagcgctttg ttaaaacctt tgattaagtc tgaatccgaa
     9061 cttacggtgg acgcaagcat aaccgcaaaa cccttttttg aacgttatgg ttttcagaca
     9121 gttaagcagc aacgcgttga atgtcgggga gagtggttta ttaattttta tatgcgttat
     9181 aaacagcaac attaaatcca gcttgcaatg aaaataacgc aagcctagta tgtgcgcgtt
     9241 ttcttattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca atgactcttt
     9301 gcagaccttt ccaggattaa ttcttttttt cttgccctgg attcgtctgc catttcctga
     9361 ttttttaatt gttactctca ataattcaag tcgcaacaca tgcaacttga agtatgacga
     9421 gtctatttac ggaatggtat atgggcaatt atattcgtcc cttatccgat gcggtattta
     9481 ccatcgcatc tgatgacctg tggatcgaga gtttagcgat ccaacaatta cacaccacgg
     9541 caaatttacc caacatgcag cgcgtagtgg ggatgccaga tttacatccc ggacgcggct
     9601 atccgattgg cgcagcgttc ttctctgttg gtcgttttta tccggcactg gtcggcaatg
     9661 atatcggctg cggtatggcg ctatggcaaa cagatattct cgctcgcaaa tacaacgccg
     9721 ataagtttga aaagcgatta tcagatctgg atgacgttgc tgaagaaagc tggctggagg
     9781 aaaacctgcc gtcagcattt gcacagcatc cgtggcgcag ctcgcttggc tctatcggtg
     9841 gcggcaatca tttcgctgaa ctgcaacaag ttgatcaaat catcaacgct gaactgtttg
     9901 cactggcagg tctggatgca caacatctgc aactgttggt tcatagcggc tcgcgggggg
     9961 tggggcagtc tattttacag cggcatattg cctcgttctc gcatcatggt ttgcctcaag
    10021 gcagtgacga cgcgctacgg tatattgcgg aacatgacga tgcgctggcg tttgcgtgta
    10081 ttaatcgcca gatgatcgct ttgcgcataa tgcaacaagt taaggccacg ggaagtccgg
    10141 ttctggatgt ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa cagggctggt
    10201 tgcatcgtaa aggtgccacg ccggatgaca acggtctggt gattattccc ggttcacgtg
    10261 gtgattactc ctggattgta cagcctgttg cgaacgaaaa aacgctccac tcgctggcgc
    10321 atggggcagg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca gcgaaataca
    10381 cggcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc gataaacaac
    10441 tcatctttga agaagcgcca caagcctata aatcggctga aagcgtggtg caatgtctgg
    10501 tgcaggccgg tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca
    10561 gtggagggaa aaaaggatga tcttgctaca actctcctca gcgcaggggc cggaagaatg
    10621 ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc gacaagacgt
    10681 cgcggtaacg gtgctggaaa cagaaacggg ccactactct gacacgctgc gttcggcgct
    10741 gatttctctg gatggcgata acgcatgggc attaagcgaa agctggtgtg gcactattca
    10801 gtggatttgt ccgagtccgt atcggcctca tcatgggcgc aaaaactggt ttctgggcat
    10861 tgggcgtttt accgctgatg agcaggaaca atcggatgca atccgttatg agacgttgcg
    10921 ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca
    10981 tttggcatcc ggtattagcg tgaaggttca gtcagagcgt agtcagcatg ctaacaagcg
    11041 gctggcacga ttgctgattg cctggaagct ggagcagcag caacaggaaa atagcgcggt
    11101 gctgaaatcg ctgcggcgaa tgttccatca ccagattgaa cgtggcaacc cgagacggac
    11161 atttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta
    11221 aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt
    11281 cagcagcgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg
    11341 accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag
    11401 accacattcc aggcccgcgt ggataatctg gatgttcggt gttttgttga acagacgctg
    11461 ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata
    11521 tgcgcctttc gcttcggttt tcgcgccagc tagtttaccc agcgaatcca gcatgctcac
    11581 cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt ctacgttatt
    11641 gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc
    11701 ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga
    11761 tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt
    11821 ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag
    11881 gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt
    11941 acgcagtgtg ccgccgttga aatcgataag gcgcagatcc agttcttccg catgacccgc
    12001 caggaagcgc accagtagtt tgttggcatt acccaggcca acgtggattt caccgccgga
    12061 gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc
    12121 ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat
    12181 ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg
    12241 taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg
    12301 gccgtgaacc acgttttcgt cagccagaac cgccagcgca gacgccatac caatgccgtt
    12361 atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat
    12421 aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg
    12481 ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag
    12541 gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat
    12601 gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa
    12661 aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt
    12721 cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa
    12781 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
    12841 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
    12901 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
    12961 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
    13021 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
    13081 gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
    13141 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
    13201 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgctgttg
    13261 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
    13321 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt
    13381 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
    13441 cactgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt
    13501 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
    13561 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
    13621 cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
    13681 tggatctggc gcggtattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg
    13741 agcgatgctg aacgtaccga cgatgattta tacgatactg tgattggcta ccgtggtggc
    13801 aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtgcggaa
    13861 gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc
    13921 gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc
    13981 gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta
    14041 cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg
    14101 acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa
    14161 cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccatc ggtgggcttt
    14221 tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg
    14281 ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc
    14341 gctaacgttg ccgtgcgtct ggcatatctt gaatcgccgc gtctgaaagc ggttgcctgt
    14401 cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa
    14461 atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga tgacgcgtta
    14521 cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg tcgctgccca
    14581 acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc
    14641 ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat
    14701 cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa
    14761 aaatttgcta aattttgcca atttggtaaa acagttgcat cacaacagga gatagcaatg
    14821 acgttaccga gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg
    14881 tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc
    14941 gtcaacgtga aaccggcacc ggaagtgcgt gagttctggg gctggtggat ggagcttgaa
    15001 gcgcaggaat cccgttttac ctacagttac cagtttggtc tgttcgataa agcaggcgac
    15061 tggaagagtg ttccggtaaa agacactgaa gtggttgaac gactggagca caccctgcgt
    15121 gagtttcacg agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg
    15181 gatgattttc gtgacgagcc ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc
    15241 acatccggca ataatcatta aaactgatag gtcatgccaa ccgcgacaat atcatcatta
    15301 ttaatattca at
//
LOCUS       DAZFRJ010000717        11188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.guided.147, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000717 DAZFRJ010000000
VERSION     DAZFRJ010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.guided.147"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..288)
                     /gene="yjeN"
                     /locus_tag="ACHKUN_005489"
     CDS             complement(<1..288)
                     /gene="yjeN"
                     /locus_tag="ACHKUN_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO6663507.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYS"
     gene            complement(340..1842)
                     /gene="yjeM"
                     /locus_tag="ACHKUN_005490"
     CDS             complement(340..1842)
                     /gene="yjeM"
                     /locus_tag="ACHKUN_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6663508.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(2061..3038)
                     /gene="epmA"
                     /locus_tag="ACHKUN_005491"
     CDS             complement(2061..3038)
                     /gene="epmA"
                     /locus_tag="ACHKUN_005491"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6663509.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            3363..5171
                     /gene="frdA"
                     /locus_tag="ACHKUN_005492"
     CDS             3363..5171
                     /gene="frdA"
                     /locus_tag="ACHKUN_005492"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6663510.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            5164..5898
                     /gene="frdB"
                     /locus_tag="ACHKUN_005493"
     CDS             5164..5898
                     /gene="frdB"
                     /locus_tag="ACHKUN_005493"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6663511.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            5909..6304
                     /gene="frdC"
                     /locus_tag="ACHKUN_005494"
     CDS             5909..6304
                     /gene="frdC"
                     /locus_tag="ACHKUN_005494"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6663512.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            6315..6674
                     /gene="frdD"
                     /locus_tag="ACHKUN_005495"
     CDS             6315..6674
                     /gene="frdD"
                     /locus_tag="ACHKUN_005495"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6663513.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            6737..7870
                     /locus_tag="ACHKUN_005496"
     CDS             6737..7870
                     /locus_tag="ACHKUN_005496"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6663514.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            7959..8492
                     /gene="blc"
                     /locus_tag="ACHKUN_005497"
     CDS             7959..8492
                     /gene="blc"
                     /locus_tag="ACHKUN_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6663515.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(8489..8806)
                     /gene="sugE"
                     /locus_tag="ACHKUN_005498"
     CDS             complement(8489..8806)
                     /gene="sugE"
                     /locus_tag="ACHKUN_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6663516.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(8988..9134)
                     /gene="ecnB"
                     /locus_tag="ACHKUN_005499"
     CDS             complement(8988..9134)
                     /gene="ecnB"
                     /locus_tag="ACHKUN_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6663517.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(9245..9370)
                     /gene="ecnA"
                     /locus_tag="ACHKUN_005500"
     CDS             complement(9245..9370)
                     /gene="ecnA"
                     /locus_tag="ACHKUN_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6663518.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(9422..9988)
                     /gene="efp"
                     /locus_tag="ACHKUN_005501"
     CDS             complement(9422..9988)
                     /gene="efp"
                     /locus_tag="ACHKUN_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6663519.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            10030..11058
                     /gene="epmB"
                     /locus_tag="ACHKUN_005502"
     CDS             10030..11058
                     /gene="epmB"
                     /locus_tag="ACHKUN_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6663520.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2894 a         2912 c         2814 g         2568 t
ORIGIN      
        1 gctatagcgc atacgaaaca aatcgccgta tcccactaat tccccgcgaa aaaccagaca
       61 tcggataaga taagcgcaat aaatatccgg caggtttccc caccgtttac catcgcgatc
      121 tttgagggtg ttaatggtgc tacccaccac catcgccact ttacgggtgt ggcaggcatc
      181 gacaaaagag agaatcgtct gctctatttc cgctacatcc ccggcagaaa attgcagcgc
      241 cctgatttca tcttcggtta ttgccgggtc ccggctggtg tcatccataa catttcctgt
      301 ctttacgccc tatcgtcagg tagacgatag ggcatcgggt tattccaccg cccactgcgg
      361 attttttgcc actctgctgc aatagttctg gtaaatcgcc atcgctaaca gcgagaagaa
      421 gacagggccg ccaatcatcc acaatgtgct gtcccagtct ccggcttcaa ccacaggttg
      481 aatgatggtg aagacgttcg caaatgtcac caccagtacg acaaccactg ttgcaatcat
      541 tgccgacata tgcgttttga aaatcacaaa tggtctgtcg agatcctgac gtgctttaaa
      601 aaatgggaaa gccagcgcga ggaacaggta aggaagcgtc atagacacgt tcgccatcag
      661 cgtcagcttg ttaaagaacg ccgatgcggt accgccacca aacgaaacca gcaggatgaa
      721 gacagtaacc aacccgcact gcatccacat ggcgatagac ggcatcccca tcgcattcag
      781 gcgcgtcatc ggttccggcc acaacgcttt cggcgtcccc tggatgatgg ctttcagcgg
      841 tgaatagcaa agcgtaaaga acgcaccggt ataagcgagg aacatcgaaa gtccggtaat
      901 acgcgcaaac catacgccca gcgacaatga cgcttcaggt gacaaatgca gtgcgttacc
      961 cagcgtcacc ccgaggctct tcatcagcac ataggtaata ttgccaaggt taacggaacc
     1021 attacttaat acctgctgcc agtttgtgct gacgccccat aaaaatattg ccagcgaata
     1081 accgattgaa ataacaatag cggcaaaaac aatacctttg gcaaagttct tttctggatt
     1141 ttccgtttta tcgaccagac caccaaccgc ttcaattccg ccataggcaa aaatagcaaa
     1201 taccacaaac gatagcattg ccagaccgga ctgataacct gggttcggtg atgcaaggaa
     1261 attaatatcc tgcgcgaaat gcccaccatt taataacaaa atagtaatgc ttactaacag
     1321 caatactaaa ttcagacaca ttactgcaat accgcccacc gcagtaatgc gggcaatttt
     1381 attaatcccc tttgaagcaa cgacggtgac cagaatcatc catgccactg ccagcagacc
     1441 aaccacctgc gtaggctcca gtccagcaat acgccagtgc tgggtcatgt cgctaccata
     1501 gaggaatgtt gagaacggta cccaaacttt cgcggaggta ctcaccatcc agatgatata
     1561 agaggaaaac cacataaacg taccaatgaa ggcaaaacgt ggtccgacac tattattcat
     1621 ccaggaatag ataccgcctt cttctttgcg ataagcagct cccatttcag ccatcattaa
     1681 ggcgaatgga ataaagaata acaatgcaga aaatatataa aagggaatcg cactataacc
     1741 cattaagtaa taagccgatg ggctattggc aaatccaaat acggaagtaa agatcatcaa
     1801 tatgagtcct atcagactca tcttttttat cgtgtgaggc atgaaaccat ccttcatttc
     1861 agcgcgtgta taacacgtgc ccactgaact gcactgcgca acattgagca ggcagcgtaa
     1921 aaaccaatac agaattgata cctggatgat atcaaatatc cagtcttaaa ttgtggcctt
     1981 taacggaaaa atgaaaacat atgctacaga atggcgctta tcacgccatt cttcgctgtt
     2041 aattcagtaa tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt
     2101 gtctccgcgc ccagcgccaa catcaccaga cgatcaacac ctaatgccac gccggaacag
     2161 tcaggcatac cgactttcaa ggcttcaatc agattctggt caatggggtg ctgcggcaaa
     2221 ccgcgcgccg cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc
     2281 gtcaattcat ggaaaccatt cgccagctca atacctttat aataaacctc aaagcgttca
     2341 gcgacccgat gatcttcggt actgatttgc gccagtgatg cctggctggc tggaaagtgg
     2401 tacacaaagg tcggtttttc tttgccaata tttggctcta cgccaaaggt aaacagcaat
     2461 tgtagcagcg tgtcgcggtc ttcttcggta tcagcaacat tgctcaaatc cagtttcgct
     2521 gcgacttccc gcagttgcgt tttgtcggca gagagcgggt caatttccag ataacgcaag
     2581 aaagcttgtt gataagaaag gctttctgct gccgggcagt ccagcacctg ttgtaagaga
     2641 tcgtccacct cgttcatcaa ccggtacata tcatagtgcg gtcgatacca ctccagcata
     2701 gtgaactcag ggttgtgata acgccccatc tcttcattac ggaagctgcg gcacagctgg
     2761 aataccggcc cacaaccggc aaccagcagg cgtttcatat ggtattccgg gctggtcatt
     2821 aaccagagat tcatcccctg cgaatgcccg gggccaacga aacgtgtctc aaacgggacc
     2881 aaatgaatat cggttaccgt cgcctggctc atacaaggcg tctccacctc cagcactcca
     2941 cgatcggcaa agaaacgacg gatctccgcc ataatcgccg cgcgttttaa taagttagga
     3001 atggatgcgc tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc agtttttgac
     3061 aagggcacga agtctactcg caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca
     3121 gcggtgggca gtgactaaaa aaagcacgat ctgatggttt agtaattaaa ttaatcatct
     3181 tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt
     3241 atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt cgcagaccgt
     3301 aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc tggaggaatg
     3361 tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg
     3421 ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat
     3481 acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc gcgcaggatc
     3541 atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg ttgtgtgagc
     3601 aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa ctggaactgt
     3661 gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca
     3721 tgaaaatcga gcgcacctgg ttcgccgccg ataagaccgg cttccatatg ctgcacacgc
     3781 tgttccagac ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg
     3841 atattctggt tgatgatggt catgttcgcg gcgtggtagc aatgaacatg atggaaggca
     3901 cgctggtgca gatccgtgct aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt
     3961 atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc
     4021 acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt ctgccaggtt
     4081 ccggtatcct gatgaccgaa ggctgccgcg gtgaaggcgg tattctggtc aacaaaaatg
     4141 gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc gagccgaaaa
     4201 acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg cacgaatggc
     4261 gtaaaggcaa caccatctcc acgccgcgtg gcgatgtggt ttatctcgac ctgcgtcacc
     4321 tcggcgagaa aaaactgcat gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg
     4381 ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac tacaccatgg
     4441 gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg
     4501 aatgttcctc tgttggtctg cacggtgcaa accgtctggg ttctaactcc ctggcggaac
     4561 tggtggtctt cggccgtctg gccggtgaac aagcgacaga gcgtgcagca actgccggta
     4621 atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc
     4681 tggttaacca ggatggcggc gaaaactggg cgaagatccg cgacgaaatg ggcctggcaa
     4741 tggaagaagg ttgcggtatc taccgtacgc cggaactgat gcagaaaacc atcgacaagc
     4801 tggcagagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca
     4861 acaccgacct gctctacacc attgaactgg gccacggtct gaacgttgct gaatgtatgg
     4921 cgcactccgc aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt
     4981 gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc gatgctgatg
     5041 gcacgactcg cctggagtac agcgacgtga agattactac gctgccgcca gctaaacgcg
     5101 tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag aaggagaagg
     5161 cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat
     5221 accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat
     5281 gcgctgggct acatcaaaga caacctggca ccggacctga gttaccgctg gtcctgccgt
     5341 atggcgattt gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa
     5401 accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt
     5461 gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg
     5521 tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag
     5581 atggcaaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc
     5641 ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt
     5701 tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag
     5761 aacggcgtat ggagctgtac tttcgtgggc tattgctccg aagtctgccc gaaacacgtc
     5821 gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg
     5881 accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc
     5941 aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg
     6001 cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa
     6061 aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg ttatcgtgat
     6121 cattaacctg atcactctgg cggcagctct gctgcacacc aaaacctggt ttgaactggc
     6181 accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa
     6241 aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg ccctgtactg
     6301 gtaaggagcc tgagatgatt aatccaaatc caaagcgttc tgacgaaccg gtattctggg
     6361 gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg
     6421 tgggtattct gctgccactg gggctgtttc cgggtgatgc gctgagctac gagcgcgttc
     6481 tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc
     6541 tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg
     6601 cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggta
     6661 tcgttacaat ctaacgtatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt
     6721 atggaaacca gaccttatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg
     6781 ctccacattt gctgcccctc aacaaatcaa cgatattgtg catcgcacaa ttaccccgct
     6841 tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta
     6901 ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt
     6961 gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg acgctattgc
     7021 tcgaggggaa atcaagttaa gcgatcccac aacaaaatac tggcctgaac ttaccgctaa
     7081 acagtggaat gggatcacac tattacatct cgcaacctac actgctggcg gcctgccatt
     7141 gcaggtgccg gatgaggtga aatcctcaag cgacttgctg cgcttctatc aaaactggca
     7201 gcctgcatgg gcgccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg
     7261 cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa cacgtgtctt
     7321 ccagccactc aaactcaacc atacgtggat taatgtaccg cccgcagaag aaaagaatta
     7381 cgcctgggga tatcgcgaag gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga
     7441 aacttatggt gtgaagtcga ccattgaaga tatggcctgc tgggtacgaa gcaatatgaa
     7501 tccccgtgat atcaacgaca aaacacttca gcaagggata caactggcac aatctcgcta
     7561 ctggcaaacc ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtaaa
     7621 tcctgacagc atcattaacg gcagtggcaa taaaattgca ctggcagcac accccgtaaa
     7681 agcgattacg cccccaactc ctgcagtacg cgcatcatgg gtacataaaa caggggcaac
     7741 cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tagtgatgct
     7801 ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctggcaga ttctcaacgc
     7861 tctacagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg
     7921 tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg
     7981 ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg
     8041 taacagtagt gaataatttc gacgccaaac gttatctcgg tacctggtat gagattgccc
     8101 gttttgatca ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg
     8161 atgacggcgg cctgaatgtc attaataaag gctataaccc tgacagagga atgtggcagc
     8221 agagtgaagg gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtgtcat
     8281 tctttggtcc tttctatggc ggttataacg ttattgcact cgatcgggaa taccgccatg
     8341 cgctggtttg cggcccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt
     8401 ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta
     8461 agtttatttg ggtacagcag cccggtagtt agtgagtgct gagtttcaga ccaataatcc
     8521 ccaatacgat taacgccaga ctcgccaggc gcatcgggtt agcggactca ccgagcagca
     8581 caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc
     8641 ctactggtaa cgatttcatc gcccaggcaa gtagcgccat actgacaatc atcgccgtca
     8701 cggtaataac actcggcgtc aaacgactaa agccgtgggt atatttcagg ccaacggccc
     8761 ataccacttc cagcagacca gcaataacta agataatcca ggacatatca ggctccggag
     8821 aattggggcc gtccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt
     8881 gaacggatat tttgccaggt ggcaggggaa tttcaactat taccttagtg actatcgcag
     8941 aaaaaacaaa cggcacgaca tacctgtcgt accgttattg cttattgcta ttgctgcgct
     9001 ttcgttgctg cgccagaaat cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc
     9061 gtggtgttgc aggcagttaa tactgttgaa agcaccagaa cagaaaagat cgctgcaatt
     9121 gtcttcttca ccataacgtc ttccttttag gtaatgttat ttatgtttgc ctatagcaaa
     9181 ttaataatag acaacatcgc caaaaatgac ggaaatctga tgatttttcg gaagagaaga
     9241 aaaattagct ggcagcgcga gagatggagt tgccgagatg tttgatgtct tcgccgaaac
     9301 cgcgagcggt gttacagccc gtgagcagcg tgctggcaag caaaacaaga acgataaggc
     9361 gtttcatcat ctctgacctg aataagtgat ggtgcagcct gcaggccgca ccacaaccgc
     9421 attacttcac gcgagagacg tattcaccag agcgggtatc cactttgatg acttcgccga
     9481 tttgtacgaa cagcggaacc ttaaccacag cgccagtaga cagggtagcc ggtttgccgc
     9541 cagtacctgc ggtatcacct ttcaggcccg gatcggtatc aacgatttcc agttcaacga
     9601 agttcggcgg agtaacggag atcggctgac cattccacag agttacgata cactctgcct
     9661 gatccagcag ccatttagcg ttgtcgccaa ttgcttttgc atcagcagac agctgctcga
     9721 aagtttcgtt gttcatgaag tgccagaact caccgtcgtt gtacaggtaa gtcaggttca
     9781 tatcgacaac atcagcgcct tcagcggaat cagtagattt gaaggttttt tctacgcgag
     9841 taccggtcag cagacgacgc agtttaacgc gagcaaatgc ctggccttta cccggtttta
     9901 cgaattcact cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta agaccagcac
     9961 gaaaatcgtt gctatagtac gttgccataa ggccctctga aatttgttaa ttggtagcta
    10021 agccacaaaa tggcgcatat tgtaacccta aataccccat ccagagaaga ttggttaacg
    10081 caacttgccg atgttgtgac cgatcctgat gaacttctgc gtcttttgaa tatagacgcg
    10141 gacgaaaaac tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc
    10201 tcatttatcg atcgcatgga gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt
    10261 acctcgcaag atgagtttgt cgtcgcgtcc ggattctcca ccgacccgct ggaagaacag
    10321 cacagcgtag tgcctggttt gttgcataaa taccacaacc gggcgctttt gctggtcaaa
    10381 ggcggctgcg cggtaaattg ccgctattgc ttccgtcgcc acttccccta tgccgaaaat
    10441 cagggcaaca agcgtaactg gcagactgct cttgagtatg ttgctgcgca tccggaactg
    10501 gacgagatga ttttctccgg cggcgatccg ctgatggcga aagatcacga gctggactgg
    10561 ttgctcacac aactggaagc catcccgcat atcaaacgtc tgcggattca cagccgtctg
    10621 ccgattgtga tcccggcacg tatcaccgag gcgctggttg aacgctttgc ccgttctacg
    10681 ctgcaaatct tgctggtgaa tcacatcaat catgccaatg aggtagatga aacgttccgt
    10741 caggcgatgg ctaaattgcg ccgtgtcggt gtcaccctgc ttaaccagag cgttctgtta
    10801 cgtggtgtga acgataacgc acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc
    10861 ggcgtaatgc cgtattacct gcatgtgctc gataaagtac agggcgcggc gcattttatg
    10921 gtgagtgatg acgaagcacg gcagattatg cgtgagttgc tgacactggt gtcgggttat
    10981 ctggtgccga aactggcgcg agaaatcggc ggcgaaccca gcaaaacgcc gctggatctc
    11041 cagctacgcc agcagtaact gattaaacag ggaagttaaa cattagcctg atgcgctacg
    11101 cttatcaggc ctacattgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga
    11161 taaggcgttt acgccgcatc cggcaaga
//
LOCUS       DAZFRJ010000718         2281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.guided.365, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000718 DAZFRJ010000000
VERSION     DAZFRJ010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.guided.365"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..887)
                     /gene="intI1"
                     /locus_tag="ACHKUN_005503"
                     /pseudo
     CDS             complement(39..887)
                     /gene="intI1"
                     /locus_tag="ACHKUN_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            1036..1874
                     /gene="aadA1"
                     /locus_tag="ACHKUN_005504"
                     /pseudo
     CDS             1036..1874
                     /gene="aadA1"
                     /locus_tag="ACHKUN_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            1991..>2281
                     /locus_tag="ACHKUN_005505"
     CDS             1991..>2281
                     /locus_tag="ACHKUN_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFO6663521.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAGTVAKLAMRQPF"
BASE COUNT          527 a          607 c          632 g          515 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc acgagtggcg gagggtgtgc
       61 ggtgtggcgg gcttcgtgat gcctgcttgt tctacggcac gtttgaaggc gcgctgaaag
      121 gtctggtcat acatgtgatg gcgacgcacg acaccgctcc gtggatcggt cgaatgcgtg
      181 tgctgcgcaa aaacccagaa ccacggccag gaatgcccgg cgcgcggata cttccgctca
      241 agggcgtcgg gaagcgcaac gccgctgcgg ccctcggcct ggtccttcag ccaccatgcc
      301 cgtgcacgcg acagctgctc gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc
      361 cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc
      421 ttgacccgca gttgcaaacc ctcactgatc cgcatgcccg ttccatacag aagctgggcg
      481 aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc
      541 accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc
      601 gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag
      661 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc
      721 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
      781 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
      841 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
      901 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      961 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     1021 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     1081 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     1141 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     1201 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     1261 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     1321 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     1381 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     1441 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     1501 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     1561 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1621 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1681 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1741 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     1801 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc
     1861 gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact
     1921 atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta cacaaattgg
     1981 gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg aagtaatcgc
     2041 aacatccgca ttaaaatcta gcgagggctt tactaagctt gccccttccg ccgttgtcat
     2101 aatcggttat ggcatcgcat tttattttct ttctctggtt ctgaaatcca tccctgtcgg
     2161 tgttgcttat gcagtctggt cgggactcgg cgtcgtcata attacagcca ttgcctggtt
     2221 gcttcatggg caaaagcttg atgcgggcac tgttgcaaag ttagcgatga ggcagccttt
     2281 t
//
LOCUS       DAZFRJ010000719         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.guided.472, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000719 DAZFRJ010000000
VERSION     DAZFRJ010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.guided.472"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..1086
                     /locus_tag="ACHKUN_005506"
     CDS             127..1086
                     /locus_tag="ACHKUN_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO6663522.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
BASE COUNT          298 a          233 c          271 g          325 t
ORIGIN      
        1 aatcggtcac tggttcgaat ccagtacaac gcgccatatt tatttaccag gctcgctttt
       61 gcgggccttt tttatatctg cgccgggtct ggtgctgatt acttcagcca aaaggaacac
      121 ctgtatatga agtgtatatt atttaaatgg gtactgtgcc tgttactggg tttttcttcg
      181 gtatcctatt cccgggaatt tacgatagac ttttcgactc aacaaagtta tgtctcttcg
      241 ttaaatagta tacggacaga gatatcgacc cctcttgaac atatatctca ggggaccaca
      301 tcggtgtctg ttattaacca caccccgccg ggcagttatt ttgctgtgga tatacgaggg
      361 cttgatgtct atcaggcgcg ttttgaccat cttcgtctga ttattgagca aaataattta
      421 tatgtggccg ggttcgttaa tacggcaaca aatactttct accgtttttc agattttaca
      481 catatatcag tgcccgatgt gacaacggtt tccatgacaa cggacagcag ttataccact
      541 ctgcaacgtg tcgcagcgct ggaacgttcc ggaatgcaaa tcagtcgtca ctcactggtt
      601 tcatcatatc tggcgttaat ggagttcagt ggtaatacaa tgaccagaga tgcatccaga
      661 gcagttctgc gttttgtcac tgtcacggca gaagccttac gcttcaggca gatacagaga
      721 gaatttcgtc aggcactgtc tgaaactgct cctgtgtata cgatgacgcc gggagacgtg
      781 gacctcactc tgaactgggg gcgaatcagc aatgtgcttc cggagtatcg gggagaggat
      841 ggtgtcagag tggggagaat atcctttaat aatatatcag cgatactggg tactgtggcc
      901 gttatactga attgccatca tcagggggcg cgttctgttc gcgccgtgaa tgaagagagt
      961 caaccagaat gtcagataac tggcgacagg cccgttataa aaataaacaa tacattatgg
     1021 gaaagtaata cagctgcagc gtttctgaac agaaagtcac agtttttata tacaacgggt
     1081 aaataaagga gttaagtatg aagaagatgt ttatggcggt tttattt
//
LOCUS       DAZFRJ010000720          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2306M38100
            SAMN43283748-rid23079043.guided.548, whole genome shotgun sequence.
ACCESSION   DAZFRJ010000720 DAZFRJ010000000
VERSION     DAZFRJ010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283748
            Sequence Read Archive: SRR30325472
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351734.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:26:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,189
            CDSs (with protein)               :: 5,189
            Genes (RNA)                       :: 101
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 96 of 218
            Pseudo Genes (incomplete)         :: 117 of 218
            Pseudo Genes (internal stop)      :: 57 of 218
            Pseudo Genes (multiple problems)  :: 44 of 218
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283748-rid23079043.guided.548"
                     /serotype="-:H11"
                     /isolate="2306M38100"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-06"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..272
                     /locus_tag="ACHKUN_005507"
     CDS             3..272
                     /locus_tag="ACHKUN_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO6663523.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(570..>809)
                     /locus_tag="ACHKUN_005508"
     CDS             complement(570..>809)
                     /locus_tag="ACHKUN_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6663524.1"
                     /translation="LWYQSDIFLPPGCIFTPTRTGVILNNKELPVTIYNVTPFNKNVW
                     NLIKSSQECPANTGNITDKCFNHRCILEICPYGRK"
BASE COUNT          213 a          150 c          212 g          234 t
ORIGIN      
        1 gtatgaagaa gatgtttatg gcggttttat ttgcattagt ttctgttaat gcaatggcgg
       61 cggattgtgc taaaggtaaa attgagtttt ccaagtataa tgaggatgac acatttacag
      121 tgaaggttga cgggaaagaa tactggacca gtcgctggaa tctgcaaccg ttactgcaaa
      181 gtgctcagtt gacaggaatg actgtcacaa tcaaatccag tacctgtgaa tcaggctccg
      241 gatttgctga agtgcagttt aataatgact gaggcataac ctgattcgtg gtatgtgggt
      301 aacaagtgta atctgtgtca caattcagtc agttgacagt tgcctgtcag actgagcatt
      361 tgttaaaaaa atttcgcatg gtgaatcccc ctgtgcggag gggcgactgg tgtataaggt
      421 gtcatctctg acgacaacct ggatcgagat acgcgggttc agggacaccg gactgaactc
      481 accgggaggc acccggcacc atgttcatgg tgatacagaa atgcggcttc agcccctctc
      541 cggaggggct ttttatctga atgattctgt tattttcgcc cgtatgggca tatttccaga
      601 atgcagcgat gattaaaaca cttatctgtg atatttcctg tgtttgcagg gcattcctgg
      661 ctgcttttaa ttaaattcca gacgttttta ttaaatgggg tcacgttgta aatggtgacg
      721 gggagctctt tattattgag tattacgcct gtccgggttg gtgtaaaaat acagcccgga
      781 gggaggaaga tatcagactg ataccataa
//
